LOCUS       DBFIFS010000001        93713 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.001, whole genome shotgun sequence.
ACCESSION   DBFIFS010000001 DBFIFS010000000
VERSION     DBFIFS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.001"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(329..1483)
                     /gene="metK"
                     /locus_tag="N7A07_000001"
     CDS             complement(329..1483)
                     /gene="metK"
                     /locus_tag="N7A07_000001"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU2474761.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            1539..1789
                     /gene="yqgD"
                     /locus_tag="N7A07_000002"
                     /pseudo
     CDS             1539..1789
                     /gene="yqgD"
                     /locus_tag="N7A07_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
     gene            1900..2043
                     /gene="yqgG"
                     /locus_tag="N7A07_000003"
     CDS             1900..2043
                     /gene="yqgG"
                     /locus_tag="N7A07_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU2474762.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            2137..2268
                     /gene="yqgB"
                     /locus_tag="N7A07_000004"
     CDS             2137..2268
                     /gene="yqgB"
                     /locus_tag="N7A07_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU2474763.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2277..4253
                     /gene="speA"
                     /locus_tag="N7A07_000005"
     CDS             2277..4253
                     /gene="speA"
                     /locus_tag="N7A07_000005"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU2474764.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            4399..5130
                     /locus_tag="N7A07_000006"
     CDS             4399..5130
                     /locus_tag="N7A07_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2474765.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            5266..6186
                     /gene="speB"
                     /locus_tag="N7A07_000007"
     CDS             5266..6186
                     /gene="speB"
                     /locus_tag="N7A07_000007"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU2474766.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6392..7150)
                     /gene="loiP"
                     /locus_tag="N7A07_000008"
                     /gene_synonym="yggG"
     CDS             complement(6392..7150)
                     /gene="loiP"
                     /locus_tag="N7A07_000008"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU2474767.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            7428..9419
                     /gene="tkt"
                     /locus_tag="N7A07_000009"
     CDS             7428..9419
                     /gene="tkt"
                     /locus_tag="N7A07_000009"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU2474768.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            9733..10176
                     /gene="cmtB"
                     /locus_tag="N7A07_000010"
     CDS             9733..10176
                     /gene="cmtB"
                     /locus_tag="N7A07_000010"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU2474769.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            10204..11592
                     /gene="cmtA"
                     /locus_tag="N7A07_000011"
     CDS             10204..11592
                     /gene="cmtA"
                     /locus_tag="N7A07_000011"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU2474770.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            11607..12884
                     /gene="yggP"
                     /locus_tag="N7A07_000012"
     CDS             11607..12884
                     /gene="yggP"
                     /locus_tag="N7A07_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU2474771.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            12881..13846
                     /gene="glpX"
                     /locus_tag="N7A07_000013"
     CDS             12881..13846
                     /gene="glpX"
                     /locus_tag="N7A07_000013"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU2474772.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            13868..14377
                     /gene="fumE"
                     /locus_tag="N7A07_000014"
     CDS             13868..14377
                     /gene="fumE"
                     /locus_tag="N7A07_000014"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU2474773.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            14374..15087
                     /locus_tag="N7A07_000015"
     CDS             14374..15087
                     /locus_tag="N7A07_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU2474774.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(15059..15736)
                     /locus_tag="N7A07_000016"
     CDS             complement(15059..15736)
                     /locus_tag="N7A07_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2474775.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(15730..16407)
                     /locus_tag="N7A07_000017"
     CDS             complement(15730..16407)
                     /locus_tag="N7A07_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2474776.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(16395..17102)
                     /locus_tag="N7A07_000018"
     CDS             complement(16395..17102)
                     /locus_tag="N7A07_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU2474777.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(17103..17681)
                     /locus_tag="N7A07_000019"
     CDS             complement(17103..17681)
                     /locus_tag="N7A07_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU2474778.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(17704..18135)
                     /locus_tag="N7A07_000020"
     CDS             complement(17704..18135)
                     /locus_tag="N7A07_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU2474779.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            18507..19526
                     /gene="epd"
                     /locus_tag="N7A07_000021"
     CDS             18507..19526
                     /gene="epd"
                     /locus_tag="N7A07_000021"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU2474780.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            19576..20739
                     /gene="pgk"
                     /locus_tag="N7A07_000022"
     CDS             19576..20739
                     /gene="pgk"
                     /locus_tag="N7A07_000022"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU2474781.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            20954..22033
                     /gene="fbaA"
                     /locus_tag="N7A07_000023"
     CDS             20954..22033
                     /gene="fbaA"
                     /locus_tag="N7A07_000023"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU2474782.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(22097..22195)
                     /locus_tag="N7A07_000024"
                     /pseudo
     CDS             complement(22097..22195)
                     /locus_tag="N7A07_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22391..23251
                     /gene="mscS"
                     /locus_tag="N7A07_000025"
     CDS             22391..23251
                     /gene="mscS"
                     /locus_tag="N7A07_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU2474783.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            23390..24025
                     /gene="argO"
                     /locus_tag="N7A07_000026"
     CDS             23390..24025
                     /gene="argO"
                     /locus_tag="N7A07_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU2474784.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            24118..24858
                     /gene="yggE"
                     /locus_tag="N7A07_000027"
     CDS             24118..24858
                     /gene="yggE"
                     /locus_tag="N7A07_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU2474785.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            25025..25921
                     /gene="ygfI"
                     /locus_tag="N7A07_000028"
                     /pseudo
     CDS             25025..25921
                     /gene="ygfI"
                     /locus_tag="N7A07_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(25918..27396)
                     /gene="scpC"
                     /locus_tag="N7A07_000029"
     CDS             complement(25918..27396)
                     /gene="scpC"
                     /locus_tag="N7A07_000029"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU2474786.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(27420..28205)
                     /gene="scpB"
                     /locus_tag="N7A07_000030"
     CDS             complement(27420..28205)
                     /gene="scpB"
                     /locus_tag="N7A07_000030"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU2474787.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(28216..29211)
                     /gene="meaB"
                     /locus_tag="N7A07_000031"
     CDS             complement(28216..29211)
                     /gene="meaB"
                     /locus_tag="N7A07_000031"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU2474788.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(29204..31348)
                     /gene="scpA"
                     /locus_tag="N7A07_000032"
     CDS             complement(29204..31348)
                     /gene="scpA"
                     /locus_tag="N7A07_000032"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU2474789.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(31552..32445)
                     /gene="argP"
                     /locus_tag="N7A07_000033"
     CDS             complement(31552..32445)
                     /gene="argP"
                     /locus_tag="N7A07_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU2474790.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            32828..33487
                     /gene="rpiA"
                     /locus_tag="N7A07_000034"
     CDS             32828..33487
                     /gene="rpiA"
                     /locus_tag="N7A07_000034"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU2474791.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            33742..34974
                     /gene="serA"
                     /locus_tag="N7A07_000035"
     CDS             33742..34974
                     /gene="serA"
                     /locus_tag="N7A07_000035"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU2474792.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            35203..35262
                     /gene="ibsC"
                     /locus_tag="N7A07_000036"
     CDS             35203..35262
                     /gene="ibsC"
                     /locus_tag="N7A07_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU2474793.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            35596..35655
                     /locus_tag="N7A07_000037"
     CDS             35596..35655
                     /locus_tag="N7A07_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2474794.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(35756..36358)
                     /gene="fau"
                     /locus_tag="N7A07_000038"
     CDS             complement(35756..36358)
                     /gene="fau"
                     /locus_tag="N7A07_000038"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU2474795.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(36379..36562)
                     /gene="ssrS"
                     /locus_tag="N7A07_000039"
     ncRNA           complement(36379..36562)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="N7A07_000039"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(36604..36933)
                     /gene="zapA"
                     /locus_tag="N7A07_000040"
     CDS             complement(36604..36933)
                     /gene="zapA"
                     /locus_tag="N7A07_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2474796.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            37101..37679
                     /gene="ygfB"
                     /locus_tag="N7A07_000041"
     CDS             37101..37679
                     /gene="ygfB"
                     /locus_tag="N7A07_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU2474797.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            37705..39030
                     /gene="pepP"
                     /locus_tag="N7A07_000042"
     CDS             37705..39030
                     /gene="pepP"
                     /locus_tag="N7A07_000042"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU2474798.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            39027..40205
                     /gene="ubiH"
                     /locus_tag="N7A07_000043"
                     /gene_synonym="visB"
     CDS             39027..40205
                     /gene="ubiH"
                     /locus_tag="N7A07_000043"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU2474799.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            40229..41431
                     /gene="ubiI"
                     /locus_tag="N7A07_000044"
                     /gene_synonym="visC"
     CDS             40229..41431
                     /gene="ubiI"
                     /locus_tag="N7A07_000044"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU2474800.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(41505..41588)
                     /locus_tag="N7A07_000045"
     CDS             complement(41505..41588)
                     /locus_tag="N7A07_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2474801.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            41879..42973
                     /gene="gcvT"
                     /locus_tag="N7A07_000046"
     CDS             41879..42973
                     /gene="gcvT"
                     /locus_tag="N7A07_000046"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU2474802.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            42997..43386
                     /gene="gcvH"
                     /locus_tag="N7A07_000047"
     CDS             42997..43386
                     /gene="gcvH"
                     /locus_tag="N7A07_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU2474803.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            43504..46377
                     /gene="gcvP"
                     /locus_tag="N7A07_000048"
     CDS             43504..46377
                     /gene="gcvP"
                     /locus_tag="N7A07_000048"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU2474804.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(46543..47976)
                     /gene="bglA"
                     /locus_tag="N7A07_000049"
     CDS             complement(46543..47976)
                     /gene="bglA"
                     /locus_tag="N7A07_000049"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU2474805.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            48021..48332
                     /gene="yqfB"
                     /locus_tag="N7A07_000050"
     CDS             48021..48332
                     /gene="yqfB"
                     /locus_tag="N7A07_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU2474806.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            48496..49155
                     /gene="yqfA"
                     /locus_tag="N7A07_000051"
     CDS             48496..49155
                     /gene="yqfA"
                     /locus_tag="N7A07_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU2474807.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(49232..50212)
                     /gene="ygfZ"
                     /locus_tag="N7A07_000052"
     CDS             complement(49232..50212)
                     /gene="ygfZ"
                     /locus_tag="N7A07_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU2474808.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            50455..50721
                     /gene="sdhE"
                     /locus_tag="N7A07_000053"
     CDS             50455..50721
                     /gene="sdhE"
                     /locus_tag="N7A07_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU2474809.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            50702..51109
                     /gene="ygfX"
                     /locus_tag="N7A07_000054"
     CDS             50702..51109
                     /gene="ygfX"
                     /locus_tag="N7A07_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU2474810.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(51149..51670)
                     /gene="fldB"
                     /locus_tag="N7A07_000055"
     CDS             complement(51149..51670)
                     /gene="fldB"
                     /locus_tag="N7A07_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU2474811.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            51782..52678
                     /gene="xerD"
                     /locus_tag="N7A07_000056"
     CDS             51782..52678
                     /gene="xerD"
                     /locus_tag="N7A07_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU2474812.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            52703..53413
                     /gene="dsbC"
                     /locus_tag="N7A07_000057"
     CDS             52703..53413
                     /gene="dsbC"
                     /locus_tag="N7A07_000057"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU2474813.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            53419..55152
                     /gene="recJ"
                     /locus_tag="N7A07_000058"
     CDS             53419..55152
                     /gene="recJ"
                     /locus_tag="N7A07_000058"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU2474814.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            55243..56341
                     /gene="prfB"
                     /locus_tag="N7A07_000059"
     CDS             join(55243..55317,55319..56341)
                     /gene="prfB"
                     /locus_tag="N7A07_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU2474815.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            56351..57868
                     /gene="lysS"
                     /locus_tag="N7A07_000060"
     CDS             56351..57868
                     /gene="lysS"
                     /locus_tag="N7A07_000060"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU2474816.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(57911..58459)
                     /gene="idi"
                     /locus_tag="N7A07_000061"
     CDS             complement(57911..58459)
                     /gene="idi"
                     /locus_tag="N7A07_000061"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU2474817.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            58514..58585
                     /locus_tag="N7A07_000062"
     CDS             58514..58585
                     /locus_tag="N7A07_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2474818.1"
                     /translation="MINQASVYRQPPVLSGCRQVKTI"
     gene            complement(58582..58707)
                     /gene="yqfG"
                     /locus_tag="N7A07_000063"
     CDS             complement(58582..58707)
                     /gene="yqfG"
                     /locus_tag="N7A07_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU2474819.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(58709..60157)
                     /gene="uacT"
                     /locus_tag="N7A07_000064"
     CDS             complement(58709..60157)
                     /gene="uacT"
                     /locus_tag="N7A07_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU2474820.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            60593..62512
                     /gene="ygfT"
                     /locus_tag="N7A07_000065"
     CDS             60593..62512
                     /gene="ygfT"
                     /locus_tag="N7A07_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU2474821.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            62512..63000
                     /gene="ygfS"
                     /locus_tag="N7A07_000066"
                     /pseudo
     CDS             62512..63000
                     /gene="ygfS"
                     /locus_tag="N7A07_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(63036..64403)
                     /gene="ghxQ"
                     /locus_tag="N7A07_000067"
                     /gene_synonym="ygfQ"
     CDS             complement(63036..64403)
                     /gene="ghxQ"
                     /locus_tag="N7A07_000067"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU2474822.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(64439..65755)
                     /gene="guaD"
                     /locus_tag="N7A07_000068"
     CDS             complement(64439..65755)
                     /gene="guaD"
                     /locus_tag="N7A07_000068"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU2474823.1"
                     /translation="MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(65773..67173)
                     /gene="xanQ"
                     /locus_tag="N7A07_000069"
     CDS             complement(65773..67173)
                     /gene="xanQ"
                     /locus_tag="N7A07_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU2474824.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(67338..70208)
                     /gene="xdhD"
                     /locus_tag="N7A07_000070"
     CDS             complement(67338..70208)
                     /gene="xdhD"
                     /locus_tag="N7A07_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU2474825.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(70205..70984)
                     /gene="ygfM"
                     /locus_tag="N7A07_000071"
     CDS             complement(70205..70984)
                     /gene="ygfM"
                     /locus_tag="N7A07_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU2474826.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(71034..72362)
                     /gene="ssnA"
                     /locus_tag="N7A07_000072"
     CDS             complement(71034..72362)
                     /gene="ssnA"
                     /locus_tag="N7A07_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU2474827.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(72365..75463)
                     /gene="ygfK"
                     /locus_tag="N7A07_000073"
     CDS             complement(72365..75463)
                     /gene="ygfK"
                     /locus_tag="N7A07_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU2474828.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(75785..76363)
                     /gene="mocA"
                     /locus_tag="N7A07_000074"
     CDS             complement(75785..76363)
                     /gene="mocA"
                     /locus_tag="N7A07_000074"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU2474829.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            76466..77236
                     /gene="yqeC"
                     /locus_tag="N7A07_000075"
     CDS             76466..77236
                     /gene="yqeC"
                     /locus_tag="N7A07_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU2474830.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            77284..78909
                     /gene="yqeB"
                     /locus_tag="N7A07_000076"
     CDS             77284..78909
                     /gene="yqeB"
                     /locus_tag="N7A07_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU2474831.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(78950..79882)
                     /gene="arcC"
                     /locus_tag="N7A07_000077"
     CDS             complement(78950..79882)
                     /gene="arcC"
                     /locus_tag="N7A07_000077"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU2474832.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(79930..81315)
                     /gene="hyuA"
                     /locus_tag="N7A07_000078"
     CDS             complement(79930..81315)
                     /gene="hyuA"
                     /locus_tag="N7A07_000078"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU2474833.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(81368..82579)
                     /gene="ygeY"
                     /locus_tag="N7A07_000079"
     CDS             complement(81368..82579)
                     /gene="ygeY"
                     /locus_tag="N7A07_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU2474834.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(82637..83833)
                     /gene="dpaL"
                     /locus_tag="N7A07_000080"
     CDS             complement(82637..83833)
                     /gene="dpaL"
                     /locus_tag="N7A07_000080"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU2474835.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(83891..85078)
                     /gene="ygeW"
                     /locus_tag="N7A07_000081"
     CDS             complement(83891..85078)
                     /gene="ygeW"
                     /locus_tag="N7A07_000081"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU2474836.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            85557..87335
                     /gene="ygeV"
                     /locus_tag="N7A07_000082"
     CDS             85557..87335
                     /gene="ygeV"
                     /locus_tag="N7A07_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU2474837.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(87375..87854)
                     /gene="xdhC"
                     /locus_tag="N7A07_000083"
     CDS             complement(87375..87854)
                     /gene="xdhC"
                     /locus_tag="N7A07_000083"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU2474838.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(87851..88729)
                     /gene="xdhB"
                     /locus_tag="N7A07_000084"
     CDS             complement(87851..88729)
                     /gene="xdhB"
                     /locus_tag="N7A07_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU2474839.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(88740..91037)
                     /gene="xdhA"
                     /locus_tag="N7A07_000085"
     CDS             complement(88740..91037)
                     /gene="xdhA"
                     /locus_tag="N7A07_000085"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU2474840.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            91453..92208
                     /gene="actS"
                     /locus_tag="N7A07_000086"
     CDS             91453..92208
                     /gene="actS"
                     /locus_tag="N7A07_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU2474841.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            92287..92360
                     /locus_tag="N7A07_000087"
     tRNA            92287..92360
                     /locus_tag="N7A07_000087"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92319..92321,aa:Gly,seq:ccc)
     gene            92594..93713
                     /locus_tag="N7A07_000088"
                     /pseudo
     CDS             92594..93713
                     /locus_tag="N7A07_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
BASE COUNT        22693 a        23276 c        24723 g        23021 t
ORIGIN      
        1 cttagttatg atttaagtgc atgaaaataa ttgagttata tcatcataga ttaaacgctg
       61 ttatctgcaa ttaagacttt actgaaaaga aatgtaacaa ctgtgaaaac cgaataataa
      121 tatggaaata ttacaaatct ttgtctgttg tggttggtgt aatcggtttc agttgtgatg
      181 ataatttgcg gtgaaacgtg atcaggaaga atgatgttat tgtgcaggcg gtttttttgc
      241 cggatgcggc gtgaacgcct atccaccgcg gtgaggtaaa agaaaaaggc cagcgcgagg
      301 ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg cgcagcagct
      361 gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg tatgctgcgg
      421 tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat gggcgcaggt
      481 cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca gtaccgaaag
      541 tttctaccat gatggaagtc ggttcagcca cgccgattgc gtaggaaacc tgaatttcac
      601 aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct gcgtaggctg
      661 cggaacggtc cacttttgat ggatctttac cagagaatgc accgccaccg tgacgcgcca
      721 tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca cccatcgggc
      781 caccgataac aaaacgaccg gtcgggttga tgaagaattt ggtggcagaa gtcagccatt
      841 cagcgggcag aatcggcttg atgatctctt ccattaccgc ttcttgcagc gatttctggt
      901 cgatctcttc agagtgctga gtggaaagca cgacagcatc gataccaacg attttgccgt
      961 cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc agagtgccgt
     1021 ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata ggtgctggca
     1081 tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca cccgcgccct
     1141 gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt ttgccgatag
     1201 cgctcagaac tgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca tagccaattt
     1261 cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg gtgatttcgc
     1321 cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt gctttcggat
     1381 cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt ttgtcaggat
     1441 gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat atcacctaaa
     1501 gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat cctaccacga
     1561 ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg attaacatct
     1621 ggatggctat tttaggtcaa ttcttcaccc gatttccact ttttttgaat cgtgtctcat
     1681 tctgttaaaa acgtggctgg aaatttttcc tgacaatgcc ggcattctgc gtatttatct
     1741 tttgcaattt tctgccattg tggggtataa aacgcggcgc gcggcttaaa taaaaagcac
     1801 acgacgtttc tttcgtgttg ccacttccag ccgggttgaa atcagagttt tggcttgtgg
     1861 gttcgtctta acaggcggcc gtggaggtga tacgaaataa tgaaccgttg tctgctgctt
     1921 aacctgtctc accgttctgg tgaagattcg ttccccgcac tctgcatctc tgctttgcat
     1981 acctgccgat gttataccca tctcggcgct tctcaggatt caagagctgg ttacagttac
     2041 tgaggactga acaagggcgc tcttgtaaaa acaagagttt tctcgtggtt tcgccgaact
     2101 gtcacactta cgttcggtta tgtgcttaat aatgttatga aaaagaaacc ggttgcgcag
     2161 ttggagcgtc agcattcact gctggaaaat ccatgtgctt atgggttgtt atcgcagttc
     2221 caggctgcga tagtcgttaa ctgttttaca cttaataaaa taatttgagg ttcgctatgt
     2281 ctgacgacat gtctatgggt ttgccttcgt cagcgggcga acacggtgta ctacgctcca
     2341 tgcaggaggt tgcaatgagc tcccaggaag ccagcaagat gctgcgtact tacaatattg
     2401 cctggtgggg caataactac tatgacgtta acgagctggg ccacatcagc gtgtgcccgg
     2461 acccggacgt cccggaagct cgcgtcgatc tcgcgcagtt agtgaaaact cgtgaagcac
     2521 agggtcagcg tctgcctgca ctgttctgtt tcccacagat cctgcagcac cgtttgcgtt
     2581 ccattaacgc cgcgttcaaa cgtgcgcggg aatcctacgg ctataacggc gattacttcc
     2641 ttgtttatcc gatcaaagtt aaccagcacc gtcgcgtgat tgagtccctg attcattcgg
     2701 gcgaaccgct gggtctggaa gccggttcca aagccgagtt gatggcagtg ctggcacatg
     2761 ctggcatgac ccgtagcgtc atcgtctgca atggttataa agaccgcgaa tatatccgcc
     2821 tggcattaat tggcgagaag atggggcaca aggtctatct ggtcattgag aagatgtcag
     2881 aaatcgccat tgtgctggat gaagcagaac gtctgaatgt cgttcctcgt ctgggcgtgc
     2941 gtgcacgtct ggcttcgcag ggctccggta aatggcagtc ctccggcggg gaaaaatcga
     3001 agttcggcct ggcggcgact caggtactgc aactggttga aaccctacgt gaagccgggc
     3061 gtctcgacag cctgcaacta ctgcacttcc acctcggttc gcagatggcg aatattcgcg
     3121 atatcgcgac aggcgttcgt gaatccgcgc gtttctatgt tgagctgcac aagctgggcg
     3181 tcaatattca gtgcttcgac gtcggcggcg gtctgggcgt ggattatgaa ggtactcgtt
     3241 cgcagtccga ctgttcggtg aactacggcc tcaatgaata tgccaacaac atcatctggg
     3301 cgattggtga tgcgtgtgaa gaaaacggtc tgccgcatcc gacggtaatc accgaatcgg
     3361 gtcgtgcagt gactgcgcat cacaccgtgc tggtgtctaa tatcatcggc gtggaacgta
     3421 acgaatacac ggtgccgacc gcgcctgcag aagatgcgcc gcgcgcgctg caaagcatgt
     3481 gggaaacctg gcaggagatg cacgaaccgg gaactcgccg ttctctgcgt gaatggttac
     3541 acgacagtca gatggatctg cacgacattc atatcggcta ctcttccggc acctttagcc
     3601 tgcaagaacg tgcatgggct gagcagcttt atttgagcat gtgccatgaa gtgcaaaagc
     3661 agctggatcc gcaaaaccgt gctcatcgtc cgattatcga cgagctgcag gaacgtatgg
     3721 cggacaaaat gtacgtcaac ttctcgctgt tccagtcgat gccggatgcg tgggggatcg
     3781 accagttgtt cccggttctg ccgctggaag ggctggatca agtgccggaa cgccgcgctg
     3841 tgctgctgga tattacctgt gactctgacg gtgctatcga ccactatatc gatggtgatg
     3901 gtattgccac gacaatgcct atgccggagt acgatccaga gaatccgccg atgctcggtt
     3961 tctttatggt cggcgcatat caggagatcc tcggcaacat gcacaacctg ttcggtgata
     4021 ccgaagcggt tgacgtgttc gtcttccctg acggtagtgt agaagtggaa ctgtctgacg
     4081 aaggcgatac cgtggcggac atgctgcaat atgtacagct cgatccgaaa acgctgttaa
     4141 ctcagttccg tgatcaagtg aagaaaaccg atcttgatgc tgaactgcaa caacagttcc
     4201 ttgaagagtt cgaggcaggt ttgtacggtt atacctatct tgaagatgaa taattaagat
     4261 tattctgctg gcgggtcgat ttatttacgt aaatcggtcc gctttctttt tatatattct
     4321 taaaaataat ttagctgctt ttcttattaa ataatctccc tacaatcgct gcattaatta
     4381 aatgcagagg gaacggttat gaaaaaatgg aaagtacgca gcgcgttggt ggcattaatc
     4441 gtgctgctgg ccggttgttc cagtaatgcg cagtataata gcagtgcatc gggcaatgtc
     4501 ggcaccgcct ggggtgggga tgtgcactca acggttcagg gcgtatctgc tgagcgagcc
     4561 tggcgcgatc cggcggagat gatcgttatt agttattcga ctaatgttcc ttccggctac
     4621 gatcgcgttt atagcattcg tattaacgaa ctggaatatg ctattcgcga tggtaatttt
     4681 aattctctac ctattacgcg tgtttatgat tcatctaata atgaaccacg ctatattgtc
     4741 catgccagag tcggaatgaa ttatcaatta tatgtccgta attacagtcg taacaccaac
     4801 tacgagatcg ttgctacggt tgatggcctg gatgtgctga acggcaagca aggttcgctg
     4861 aataacaacg gctatattgt gaatgccgga gattcgttag cgattaaagg attccgcaaa
     4921 gataaacata ccgaagcggc ttttcagttc gccaacgttg ctgattccta cgcagccaat
     4981 tcggcgcaag gcgatgtgcg taataccggt gtaatcggct ttgccgcctt tgaattacaa
     5041 ggccaggcgc aaaacgcgct gccaccgtgc tctggacaag cctttccggc agataacaat
     5101 ggctatgcgc cgccaccgtg tcggaaatag caacctcgct tgatgcctcg cccatttaag
     5161 gcgataatta gcacaaactt gtgtttcgca aatccaaccc ttcctcgtcg ggcctaacga
     5221 cgcggaaggg tttttttata tcgactttgt aataggagtc catccatgag caccttaggt
     5281 catcaatacg ataactcact ggtttccaac gcctttggtt ttttgcgcct gccgatgaac
     5341 ttccagccgt atgacagcga tgctgactgg gtgattactg gtgtgccgtt cgatatggcc
     5401 acttctggtc gtgcgggggg acgccacggt ccggcagcta tccgtcaggt ttcgacgaat
     5461 ctggcctggg aacacaaccg cttcccgtgg aatttcgaca tgcgtgagcg tctgaacgtc
     5521 gtggactgtg gtgatctggt atatgctttc ggcgatgccc gtgagatgag cgagaagctg
     5581 caggcgcacg ccgagaagct gctggctgcc ggtaagcgta tgctctcctt cggtggtgac
     5641 cactttgtta cgctgccgct gctgcgtgct catgcgaagc atttcggcaa aatggcgctg
     5701 gtacactttg acgcccacac cgatacctac gcgaacggtt gtgaatttga ccacggcacc
     5761 atgttctata ccgcgccgaa agaaggtctg atcgacccga atcattccgt gcagattggt
     5821 attcgtaccg aatttgataa agacaacggc tttaccgtgc tggacgcctg ccaggtgaac
     5881 gatcgcagcg tggatgacgt tatcgcccag gtgaaacaaa ttgtgggtga tatgccggtt
     5941 tacctgacct ttgatatcga ctgcctggat cctgcatttg cgccgggaac cggtacgccg
     6001 gtaattggcg gtctgacctc cgatcgcgct attaaactgg tacgcggcct gaaagatctc
     6061 aacatcgttg ggatggacgt agtggaagtg gctccggcat acgatcagtc ggaaatcacc
     6121 gctctggcag cggcaacgct ggcgctggaa atgctgtata ttcaggcggc gaaaaagggc
     6181 gagtaagcac cagatgcgat gcgcacgggt aaaacgtgcc attaatgtcg gatgcggcgt
     6241 gaacgcctta tccgacctac gttcggcacc cgtaggccgg ataagatgcg ccagcatcgc
     6301 atccggcaat gcgcacaagg taacaaatgt gccattcatg tcagatgcgg cgtgaacgcc
     6361 ttatctgacc tacgttcgac accaccaggc tttacttaat cccatccgcg ctcatgcgat
     6421 cgcgaatatg ctgggcgcgt tcggcggatg caggatggtc gtcaaacatt gagctttggc
     6481 gaccttcttc cagttttgcc agtttttcaa agctggtggc aagacctgcc gggctgatgc
     6541 cgcgttggcg cagaagatcg taagaataat catcggcttc tgcttcctgg cgctgggaga
     6601 attgcgaatt aactaatttc tcgcccagat caccgagttg tgattgagat aaactgccga
     6661 caatcccgcc cgccgaggcc gcagctactc gtacggcatt tgtaccaagt gccacctgca
     6721 ttcctttttt cacatggcct aatgccacgt gccccatttc gtgaccgatc accgcttcga
     6781 cttcgttatc cgtcatcata tccatcagcc cgctatagac acggatacag ccgttagcca
     6841 ttgcaaaggc gttcacatcc ttcgccatat acactttgta atttaccggc tgaccgttga
     6901 tattgttgcc tagcgcattg gcaatagttg tcagacgttt agcgtattcg ctattggctg
     6961 gcgcaatcgt cgccttgctg tccatctcct gacatgcctg atcgctcagg gttttcacct
     7021 gcgcatcact caaactgtaa gcctgaaaag cttccgctcc tgatgagagc agtccgttgg
     7081 agtccatatt ctggcaaccg gtcagtaccg ttgccacgct cattgctacc aataaggcgc
     7141 gaattttcat gttttttctt ccgcacaagg tcaggattat tctaaaaatg cgccgtttgc
     7201 aggtgaaccg acgctcagtc tcagtataag gaaaagcgca gcggacgggc gagtagattg
     7261 cgcaacatgc gagcatgatc cagagatttc tgaagcggca aaaggatgtt ccatgtacat
     7321 gacgcgcggc ttgcggtaaa ttgttggcaa attttccggc gtagcccaaa acgcgctgtc
     7381 gtcaagtcgt taagggcgtg cccttcatca tccgatctgg agtcaaaatg tcctcacgta
     7441 aagagcttgc caatgctatt cgtgcgctga gcatggacgc agtacagaaa gccaaatccg
     7501 gtcacccggg tgcccctatg ggtatggctg acattgccga agtcctgtgg cgtgatttcc
     7561 tgaaacataa cccgcagaat ccgtcctggg ctgaccgtga ccgcttcgtg ctgtccaacg
     7621 gccacggctc catgctgatc tacagcctgc tgcacctcac cggttacgat ctgccgatgg
     7681 aagaactgaa aaacttccgt cagctgcact ctaaaactcc gggccacccg gaagtgggtt
     7741 acaccgctgg tgtggaaacc accaccggtc cgctggggca gggtattgcc aacgcagtag
     7801 gtatggcgat tgcagaaaaa acgctggcgg cgcagtttaa ccgtccgggc cacgacattg
     7861 tcgaccacta cacctacgcc ttcatgggcg acggctgcat gatggaaggc atctcccacg
     7921 aagtttgctc tctggcgggt acgctgaagc tgggtaaact gattgcgttc tacgatgaca
     7981 acggtatttc tatcgatggt cacgttgaag gctggttcac cgacgacacc gcaatgcgtt
     8041 tcgaagctta cggctggcac gttattcgcg acatcgacgg tcatgacgca gcatccatca
     8101 aacgcgcagt agaagaagcg cgcgcagtga ctgacaaacc gtccctgctg atgtgcaaaa
     8161 ccatcatcgg tttcggttcc ccgaacaaag ccggtactca cgactcccac ggtgcgccgc
     8221 tgggtgatgc agaaatcgct ctgacccgcg aacagctggg ctggaaatac gcaccgttcg
     8281 aaatcccgtc tgaaatctat gcgcagtggg atgcgaaaga agcaggccag gcgaaagaat
     8341 ctgcatggaa tgagaagttt gcggcttacg cgaaagctta tccgcaggaa gcggctgaat
     8401 ttacccgccg tatgaaaggc gaaatgccgt ctgacttcga cgccaaagcg aaagagttta
     8461 tcgctaaact gcaggctaat ccggcgaaaa tcgccagccg taaagcgtct cagaatgcta
     8521 tcgaagcgtt cggcccgctg ttgccggaat tcctcggcgg ctccgctgac ctggcaccgt
     8581 ctaacctgac cctgtggtct ggttctaaag caatcaacga agatgctgcg ggtaactaca
     8641 tccactacgg tgttcgcgag ttcggtatga ccgcgattgc taacggtatc tccctgcacg
     8701 gtggcttcct gccgtacacc tccaccttcc tgatgtttgt cgaatacgca cgtaacgccg
     8761 tacgtatggc tgcgctgatg aaacagcgtc aggttatggt ttacacccac gactccatcg
     8821 gtctgggcga agatggcccg actcaccagc cggttgagca ggttgcttct ctgcgcgtga
     8881 ccccgaacat gtctacatgg cgtccgtgtg accaggttga atccgcggtc gcgtggaaat
     8941 acggtgttga gcgtcaggac ggtccgaccg cgcttatcct ctcccgtcag aacctggcgc
     9001 agcaggaacg tactgaagag caactggcaa acatcgcgcg cggtggttat gtgctgaaag
     9061 actgtgctgg tcagccggaa ctgatcttca tcgcgaccgg ttcggaagtt gaactggctg
     9121 ttgccgccta cgaaaaactg actgccgaag gcgtgaaggc gcgcgtggtt tccatgccgt
     9181 ctaccgatgc attcgacaag caggatgcgg cttaccgtga atccgtactg ccgaaagcgg
     9241 ttactgcacg cgttgcggta gaagcgggta ttgctgacta ctggtacaag tatgttggcc
     9301 tgaatggcgc tatcatcggt atgaccacct tcggtgagtc agctccggca gagttgctgt
     9361 tcgaagagtt cggcttcacc gtcgacaacg tcgttgcgaa agcaaaagaa ctgctgtaac
     9421 tagcatttcg ggtaaaaagg tcgcttcggc gacctttttt attaccttga tatgtccgtt
     9481 tgcggacaag caatagataa agcgtgttgt agatcgcaaa tatttatatg caataaatat
     9541 caattatgta atatgcatca cgatatgcgt gttgacattt gttgttatat ctataactca
     9601 atgttatata agaaattaat aattcactgt tttcaaaaca ccggtttccc tgctcaattg
     9661 ctttcattaa accgcacgag tattttattt ttaccgccgg acggtaccct aaaaataaaa
     9721 taaggataat ttatgcggct tagtgactat tttccagaat catcaatctc agttatacat
     9781 tcagcaaaag attggcagga agctatcgat ttctcgatgg tatcattgct ggataaaaac
     9841 tatatcagcg agaattacat tcaggcaatt aaagattcca ccattaacaa tggcccttat
     9901 tatattctcg caccaggcgt ggcaatgcct catgcgcgac cggaatgtgg ggcgcttaaa
     9961 accgggatgt ctttgacatt acttgaacaa ggtgtttatt ttccggggaa tgacgaacca
    10021 attaaattac tcatcggact ctctgctgcc gatgccgatt cgcacattgg cgctattcag
    10081 gcgttaagtg aattactgtg cgaagaagaa atactcgaac aactcttaac agcatcatca
    10141 gaaaaacaat tagcggacat tatcagccgc ggataattac tctctcctct acaggataat
    10201 attatggaaa acaagtctgc tcgtgcaaag gtccaggctt ttgggggctt tttgactgca
    10261 atggtcatcc ccaatattgg tgcttttatt gcctggggtt ttattactgc gttatttatt
    10321 cccaccggtt ggctgcctaa cgaacatttc gccaaaattg tcggcccgat gattacctat
    10381 ttattgcccg tgatgattgg ttctaccggt ggtcatctgg tcggcggtaa acgcggcgcg
    10441 gtcatgggcg gaataggtac tattggtgtg atcgtcggcg cagagatccc gatgttcctt
    10501 ggctcaatga ttatggggcc gctcggtggt ctggtcataa aatatgttga taaggcactg
    10561 gaaaaacgca tacctgccgg ttttgagatg gttatcaata acttctcatt aggtatcgcg
    10621 gggatgctcc tttgcctgct gggttttgaa gtgatcggcc cggcggtgtt aattgccaat
    10681 actttcgtca aagagtgtat tgaggcgctg gtacatgcgg gttatctgcc tctgctgtca
    10741 gtcatcaatg aaccggcgaa agtgcttttc cttaataatg cgatcgatca gggcgtctat
    10801 tatccgctgg gaatgcaaca ggcttcggtt aacggtaaat ccatcttctt tatggtggcc
    10861 tctaacccag gtccgggcct ggggctgctg ctggcgttta ccttgtttgg taaagggatg
    10921 agtaaacgtt ctgcgcccgg ggcgatgatt attcacttcc tcggtgggat ccacgaactg
    10981 tatttcccat atgtgctgat gaagccgctg actattattg ccatgattgc gggcggtatg
    11041 tctggcacct ggatgtttaa cttactggac ggtggtctgg tggctggccc aagtccgggg
    11101 tctatctttg cttacctggc gctgacgccg aaaggttcgt tcctggcgac aattgccggt
    11161 gttacggtag gtaccctggt gtcctttgct atcacttcgc tgatactgaa gatggaaaaa
    11221 acggtggaaa cggagagcga agatgagttt gctcagtcag ccaatgcggt taaggcgatg
    11281 aaacaagagg gtgcattctc gttaagcagg gttaagcgta tcgcctttgt ttgcgatgcg
    11341 gggatgggct ccagtgcgat gggcgcgacc accttccgta aacgcctgga aaaagcgggg
    11401 ctggcaattg aagtaaaaca ttacgccata gaaaacgtgc ctgcggatgc ggatatcgtc
    11461 gttactcatg ccagtctgga agggcgcgtg aaacgtgtga cggataaacc actgatattg
    11521 attaataact atattggcga tccaaaactc gacactttat ttaatcaatt aaccgccgaa
    11581 cataaacact gattacagag gtaaaaatga aaaccaaagt tgctgctatt tatggcaagc
    11641 gggatgtccg tctgcgcgaa tttgaactgc cagaaattac cgataatgaa ttactggtga
    11701 gtgtgatttc tgacagcgtc tgtttatcca cctggaaagc ggcgttactc ggtagtgaac
    11761 ataaacgcgt acccgacgat ttagaaaatc atccggtcat taccgggcat gaatgtgccg
    11821 gggttattgt cgaagtgggt aaaaatctca ctggcaaata taaaaaaggc cagcgttttg
    11881 tattgcaacc ggcgatgggg ttaccaagcg gatattcagc gggctacagc tacgaatatt
    11941 ttggcggcaa tgccacttat atgattattc ccgaaatcgc cattaatttg ggctgcgtat
    12001 taccgtatca cggctcttat tttgctgcgg cgtcgctggc agagcctatg tgctgcatta
    12061 ttggcgctta tcatgccaat tatcacacca cgcaatatgt ttatgagcat cgcatgggcg
    12121 tcaaacctgg cggcaatatt gcactgctgg cgtgtgcagg tccgatgggc attggcgcta
    12181 tcgattacgc cattaacggc ggcatacaac cgtcgcgggt ggtggtggtc gatatcgacg
    12241 acaaacgtct ggcgcaggta cagaagctgc tgccggtgga actggcggcc agtaaaggca
    12301 ttgagctggt gtatgtgaat accaaaggga tgagcgatcc tgtccagatg ctgcgggcgc
    12361 tgacaggaga tgccgggttc gatgacattt ttgtttatgc ggcggtgtct gctgtcgttg
    12421 agatggctga tgaattactg gcggaagatg gctgtctgaa cttctttgcc gggccgacgg
    12481 ataaaaactt caaagtgccg tttaatttct acaacgttca ttacaacagc acgcacgtcg
    12541 tcggtacatc cggcggttca acggacgaca tgaaagaggc gattgccctt agcgccacag
    12601 ggcagttaca gccctcgttt atggtgaccc atatcggtgg cctggatgcg gtgccagaaa
    12661 ccgtgctcaa tctgccggat atccctggcg gtaaaaaact catttataac ggcgtgacca
    12721 tgccgctcac tgccattgcc gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag
    12781 agttggcgcg gctggttgag gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc
    12841 tgctggcaca atttggcgtt gatatcgggg aggccgcgca atgatgtccc tggcgtggcc
    12901 attatttcgc gttacggaac aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga
    12961 caaaaataaa attgatggcc tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc
    13021 ttttcgtgga cgagtggtta tcggcgaagg agaaattgac catgcgccga tgctgtggat
    13081 tggcgaagag gtgggcaaag gcgatgggcc agaagtcgat atcgcggttg atcccattga
    13141 aggcacgcgg atggtggcga tggggcagag caatgcgctg gcggtcatgg ctttcgcccc
    13201 tcgagatagc ctgttacatg cgcccgatat gtatatgaaa aagctggttg ttaatcgact
    13261 ggctgccggg gcgattgatc tgtctttgcc tctggcggac aacttgcgtg acgtggcgcg
    13321 ggcgttaggt aaaccgctgg acaagctgcg catggttacg ctggataaac cgcgtctgag
    13381 tgccgcgatt gaagaagcaa cacagctggg cgtgaaggtt tttgccctgc cggatggcga
    13441 tgtcgccgcc agcgtgctga cctgctggca ggataatcct tacgatgtga tgtacaccat
    13501 cggcggtgcg ccggaaggcg tgatttctgc ctgtgccgtt aaagcattag gcgggaatat
    13561 gcaggccgag ctgattgact tttgtcaggc caaaggggat tacacggaaa atcggcagat
    13621 tgcagagcag gagcgcaagc gttgcaaggc aatgggtgtt gacgttaacc gtgtgtactc
    13681 gctcgatgta ctggtaaggg ggaacgatat cctctttagc gctacgggcg tcacgggcgg
    13741 cgagttggtg aacggtatcc aacagacggc gaatggagtg cggacgcaga cattactgat
    13801 cggcggcgcg gaccaaacgt gtaatataat agactccctg tattgatggt gatctatcgg
    13861 aagaccaatg gcgacgctgt cagaagatga tgtgcttgag caactggatg cacaggacaa
    13921 tttattttca tttatgaaaa ctgcgcattc tattttgctc caggggatac gccagtttct
    13981 gccgtcgctg tttgtcgata acgatgaaga gagcgtcgaa tatgcagtga agccgttact
    14041 cgcccaaagc ggcccgcttg acgatattga tgttgcactg cgtttgattt atgcgctggg
    14101 gaaaatggat aaatggctgt acgccgatat cacgcatttt tcccagtact ggcattacct
    14161 gaaagaacag gatgaaacgc tcggatttgc cgatgacatg acttgggatt ttatcagcaa
    14221 cgtcaatagc ataacctgca atgcgacgct ctatgacgcg ttgaaagcga tgaagttcgc
    14281 cgatttcgcc gtctggtcag aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct
    14341 ggcagtaacg accaccttaa aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg
    14401 ctgaacgtgc aagcgcagta ccatgatgaa gagatagaac gcgtgcataa accgttgctg
    14461 cgtatgctgg cggccttaca gacggtaaat ccgcagcgcc gaaccgtggt ttttctttgc
    14521 gctccgccgg ggacgggtaa atctacccta accaccttct gggcatatct cgcgcaacaa
    14581 gacccggagc tgcccgctat tcagacgctc ccgatggatg gttttcacca ttacaatagc
    14641 tggctggatg cgcatcaatt gcgccccttc aaaggcgcac cagagacatt tgacgttgcg
    14701 aaactggcgg aaaatctgcg ccaggtcgtg gaaggggatt gtacgtggcc gcagtacgat
    14761 cgacaaaagc atgatcctgt tgaagatgcg ttgcacgtta ccgcaccaat aatcatcgtc
    14821 gaaggaaact ggctattgct ggatgacgat aactggcgcc agctagcgca attttgtgat
    14881 ttctcgatct ttatcaatgc gccagctact gcgttgcggg aacgactggt ggggcgcaag
    14941 ctggcgggag ggttatcgct ggcagacgca gaagcttttt atgaccgtac cgacgggccg
    15001 aatgttcgcc gggtgctgga agagagtctg cctgcaaatt tgaccttaat gatgaccgct
    15061 acgggtgaat atcgtctggt ggattaatgt tcctgattag accatcttcc agtcgcacca
    15121 cacgagagaa atgacgccgg gtaaatgcgg cgtcatgact gattgctacg acgctcgtgc
    15181 ctcgctggcg gcacttctcc agccagcttt caaacacgct caaccaattt tcgtcaaaat
    15241 cccgactcgg ctcgtccagc agcaatagtg gcggagagag tgcttccagg caggcgacgg
    15301 cgaccatccg tcgctgcgca ctatgcagat ccaacgggtg cgcgctggca gtgtctgcta
    15361 actgacaaca ttgcagggca gcatgggtac gttgagtgat ttcatccgcc gggcattttt
    15421 gcagtttcaa accaaatgcg atctcgtcag cgacggtgct gtgaaaaagt tggttttccg
    15481 cttcctgaaa cagtacgcca atctttgccg cgcgctggcg gtttttcagg tttttcatcg
    15541 cctgttgttg cagcataacc gtaccggctg tgggcgtaag aagacctgcc attactcgta
    15601 acagcgtcga tttccctgcg ccattgtcgc cggtcaacgc caaccattca ccttgtttga
    15661 gttgcagaga aatgtcgcaa agacaatccg ttgccgcgcc aggccagcga taagatatct
    15721 gatttaacgt taacatagtg gctggagcgc tccgttttgc aattgccacg cttgctggca
    15781 gtagttcagg aaaggtgtgc gatgacgttc aaaaagaata atcagcgaac cacgttcaaa
    15841 cgcccagtgt tgtaagcgct gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa
    15901 ggcttcatca agaattaata gctttggctg catcgcgatg gcgcaggcga tgaccacgcg
    15961 ctgtgtctct ccaccggaga gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt
    16021 cagagtcagg gcggcatcaa tccgcgccat aatctctgct tcagcaagac acagattttc
    16081 tggcccaaag gcaacttctt cttccacgct aaaggtacag ccggagagct gcaaataggg
    16141 cgattgctgg acgagctgga tggtggcgga ttgttcatta agcgggagat gcccaatggg
    16201 cgtaccgagg agcgtccccg tgcctgttat ctctcctggc agaaagtccg gataccagcc
    16261 cgccatcagc tgtgccagcg tacttttgcc actgccgttg tcaccaaaga tggcgaccat
    16321 ccccggtttg acatagtgaa agtcataaca gaacggagga tgcgttgagt gtgtcgggca
    16381 ataacggaac tgttctaacg taaccatatc cacgctccga attctgtcag catcagcaag
    16441 atcatggtgt aacgcgctac gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt
    16501 cgtcggttgt ggatccgaaa tgcccgcata tccagcgccg caccacggac agcaagatcg
    16561 tttagcgcat tgtgtgttag cggaatagtt aacgcgggca tcgcacgtaa acgctgatac
    16621 cagccctcat ccagcggcac gccgcgggca cgctgcgctt catgaataat cgctaattgc
    16681 cgttttaact gctcaacgac caataacggc ccggcaaaca gataagcgac gcccggcggc
    16741 aggcgagaag caaacagagc gcggataaaa cattgcacgg ggacgtactg catccacaac
    16801 tgtgaggttg aaacaatcgc cagaatccgc aaccagagcg taatagcatg cgtccaacgc
    16861 tccggcgaac gcggcgtgcc gcttagccat tctgttagcc agccaccgtg taccagccac
    16921 agcccggctc ccagcgagaa catcagccag acgacatatt ttgcccgccg ccgcgtggcc
    16981 tttagcgcaa taaggcaaaa aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg
    17041 ggcaggataa gcgtggtgca ggccgccaat gcccatagtg ttaatgaggt aaatggatgc
    17101 atttagcgca cggcagccat tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc
    17161 agtaaaagcc agacaatcac cgctgtaaga gttttatcga ccaggttcgc gcctatcacc
    17221 gtaatcgcca ccgactccac caggttttgc cccatcgagt gcatccaggc aacgaacaaa
    17281 tcggctccgc taccggtgac accgccgaac agcgcagtac gcagcggtac agcgacgacc
    17341 gttaccgcaa gcgtaataat cacgccgctg acaacgactt ttggcagggt gcgaaaccaa
    17401 cccgctcgcg ccagccaacc ggcgaccagg ccgatgacca tcgcgacggg ggcaaaagcg
    17461 gcagcgatcg ggtcggtcag taatccccag agtaaattgg tcagtaagcc ggtcagcatc
    17521 ccgatcaccg gaccaagcag taccgcgcta ataagtgtgc cgatggagtc gagaaatata
    17581 ggtaatttga ccatgctggc cagttgcccg ccgatcatat tgatggcgat agatataacg
    17641 atcaacacca gtgcctgact ggaaaagtgg ctacgcgcca taagaaaacc tctctgaaag
    17701 tagttattta acgggggaaa tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg
    17761 gctaaacgcc agttcctcaa ggctaccgat caccagttca agcgtggtcc gcacggtaca
    17821 gccgggcatc atatgaccgc caagcatcgt gccgtgtgga tcggaaacgc agagatgaag
    17881 atgctcacca ctctgttcca acgtgccgtt tagtgagatc acttcgaatg taccgttcag
    17941 gagcgtggtg ccttcttgcc cggcatagcg caaggcgatg tcagtcaggc taccggtaca
    18001 gcctgcaatc caggcggcat gaagttgctg ctgctgtgca aaagcgtgta gctgggaaaa
    18061 aacttcctgc ccggggagga ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta
    18121 gggcaatgtc atcattggag tactccgaaa ctcggacgta ttacggataa tatcgacgct
    18181 gcatcagtca tattctgata taagtcaggg tttgtgacgg cacaacgaaa agattttgct
    18241 acgcttattc gtgattctgc aaagtggaat aaattccaca ggggcggggc tgaaaacgtg
    18301 acgtaagtca gataattgtt ttctttgact ggacaatcat tccttttatt ccacgtttcg
    18361 cttatcctag ctgaagcgtt tcagtcgatt aaatgttcga caattaacca atcagtcgca
    18421 gtttgcgaca ggtaaggttt ccccggacga tttgctggat tactctgtct ggcaaatttg
    18481 ctcagggaaa accttgcagg agatctatga ccgtacgcgt agcgataaat ggcttcggtc
    18541 gcatcgggcg taatgtggtt cgtgctttgt atgaatccgg acgccgggcg gaaattaccg
    18601 tggtggcaat caacgaactg gcggatgctg cgggcatggc gcatttgttg aaatacgaca
    18661 ccagccatgg ccgttttgca tgggaagtac gacaggaacg cgatcaactt tttgttggtg
    18721 atgacgccat ccgcgtattg catgaacgtt cactgcaatc gctcccctgg cgtgaacttg
    18781 gcgttgatgt agtcctcgac tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc
    18841 acattgccgc cggggctaaa aaagtgctct tttcacatcc tggcagtaac gatctcgacg
    18901 cgaccgttgt ttacggcgtc aatcaggatc aacttcgtgc ggagcaccgc atcgtttcta
    18961 acgcttcctg taccacgaat tgcataattc ccgtcatcaa attgttagat gatgcgtacg
    19021 gtattgagtc cggcactgtg accacaattc actccgccat gcacgatcaa caggttattg
    19081 atgcatacca tcctgacctg cgtcgcaccc gggcagccag ccagtcgatc attccggtcg
    19141 atactaaact ggccgccggt atcacacgat tttttccgca atttaacgat cgctttgaag
    19201 cgattgcggt acgtgtgcca accataaatg tgacggcaat cgatttaagc gtgacggtga
    19261 agaaacctgt aaaagccaat gaagtcaacc tgttgctgca aaaagcagca caaggtgcat
    19321 ttcatggtat agttgactat acggaattgc cgttggtctc tgtagatttt aaccacgatc
    19381 cgcacagtgc cattgtcgat ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa
    19441 cgttggtctg gtgcgataac gaatggggct ttgctaaccg aatgctcgac acgacgttag
    19501 ctatggctac tgttgctttc aggtaagacg caagcagcgt ctgcaaaact tttagaatca
    19561 acgagaggat tcaccatgtc tgtaattaag atgaccgatc tggatcttgc tgggaaacgt
    19621 gtatttatcc gtgcggatct gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg
    19681 cgtatccgtg cttctctgcc gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg
    19741 gtaacttccc acctgggtcg tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg
    19801 ccggttgtta actacctgaa agacaaactg tctaacccgg ttcgtctggt taaagattac
    19861 ctcgacggcg ttgacgttgc tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac
    19921 aaaggcgaga agaaagacga cgaaatcctg tccaaaaaat acgctgcact gtgtgacgtg
    19981 ttcgtaatgg acgcattcgg tactgctcac cgcgcgcagg cttctactca cggtatcggt
    20041 aaattcgctg acgttgcgtg cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt
    20101 aaggcactga aagaacctgc tcgcccgatg gtggctatcg ttggtggttc taaagtatct
    20161 accaaactga ccgttctgga ctccctgtct aaaatcgctg accagctgat tgttggtggt
    20221 ggtatcgcta acacctttat cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa
    20281 gctgacctgg ttgacgaagc aaaacgtctg ctgaccacct gcaacatccc ggttccgtct
    20341 gatgttcgcg tagcaaccga gttctctgaa accgctccgg ctaccctgaa atctgttaac
    20401 gacgtgaaag ctgacgagca gatcctggat atcggtgatg cttccgctca ggaactggct
    20461 gaaatcctga agaatgcgaa aaccattctg tggaacggtc cggttggcgt gttcgaattc
    20521 ccgaacttcc gcaaaggtac tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc
    20581 tccatcgctg gcggcggcga cactctggca gcaatcgacc tgttcggcat tgctgacaaa
    20641 atctcctaca tctccactgg cggcggcgca ttcctcgaat tcgtggaagg taaagtactg
    20701 cctgcagtag cgatgctcga agagcgcgct aagaagtaaa aaatcacagg gcagggaaac
    20761 ctgcccttgt ttcagcgcgc ttttagagca cgcatttttt catactctaa ataattcgag
    20821 ttgcaggaag gcgacaagag agtgaatccc caggagctta cataagtaag tgactggggt
    20881 gagcgaacgc agacgcagca catgcaactt gaagtatgac gagtataagg cccgacgata
    20941 caggacaaga gacatgtcta agatttttga tttcgtaaaa cctggcgtaa tcactggtga
    21001 tgacgtacag aaagttttcc aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa
    21061 ctgcgtcggt actgactcca tcaacgccgt actggaaact gctgctaaag ttaaagcgcc
    21121 ggttatcgtt cagttctcca acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc
    21181 tgacgttccg cagggtgctg ctatcctggg cgcgatctct ggtgcgcatc acgttcacca
    21241 gatggctgaa cattatggtg ttccggttat cctgcacact gaccactgcg cgaagaaact
    21301 gctgccgtgg atcgacggtc tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa
    21361 gccgctgttc tcttctcaca tgatcgacct gtctgaagaa tctctgcaag agaacatcga
    21421 aatctgctct aaatacctgg agcgcatgtc caaaatcggc atgactctgg aaatcgaact
    21481 gggttgcacc ggtggtgaag aagacggcgt ggacaacagc cacatggacg cttctgcact
    21541 gtacacccag ccggaagacg ttgattacgc atacaccgaa ctgagcaaaa tcagcccgcg
    21601 tttcaccatc gcagcgtcct tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt
    21661 tctgactccg accatcctgc gtgattctca ggaatatgtt tctaagaaac acaacctgcc
    21721 gcacaacagc ctgaacttcg tattccacgg tggttccggt tctactgctc aggaaatcaa
    21781 agactccgta agctacggcg tagtgaaaat gaacatcgat accgataccc aatgggcaac
    21841 ctgggaaggc gttctgaact actacaaagc gaacgaagct tatctgcagg gtcagctggg
    21901 taacccgaaa ggcgaagatc agccgaacaa gaaatactac gatccgcgcg tatggctgcg
    21961 tgccggtcag acttcgatga tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat
    22021 cgacgttctg taagtcattc ctttctgctt atctcaaggc ccgctctgcg ggtctttttt
    22081 tcgccaaaag caaaattgcc tgatgcgcta tgcttatcag gcctacacgt atcctgcaat
    22141 atattgaatt tgcaggtttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgac
    22201 aaagcgcaca ttgatatctg tctgagcccc gctctgcggg gatttttttg ccaaaagcaa
    22261 ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct
    22321 tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa
    22381 ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct
    22441 ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat
    22501 catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat
    22561 ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggtat
    22621 tatcgccttt acgctaatcg cagccctggg acgcgtgggt gtacaaacag cgtcagtcat
    22681 tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactttctaa
    22741 cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga
    22801 cctcggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac
    22861 tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa atattattaa
    22921 cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt atgattccga
    22981 tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc gcattttgaa
    23041 agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta atttcgtggt
    23101 ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat
    23161 taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt
    23221 taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg
    23281 gcctctctct tattagttat tctgattgcc aattaatatt atcaatttcc gctaataaca
    23341 atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg tgttttctta
    23401 ttactttcaa ggtcttgctc ttggggcagc tatgattctg ccgctcggcc cacaaaatgc
    23461 ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt atgattgcct tactttgcgc
    23521 tatcagcgat ttggtcctga tttgcgcggg gatttttggt ggtagcgcgt tattgatgca
    23581 gtcgccgtgg ttgctggcgc tggtcacctg gggcggcgta gtcttcttgc tgtggtatgg
    23641 ttttggcgct tttaaaacag cgatgagtag taatattgag ttagctagtg ccgaagtcct
    23701 gaagcaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca
    23761 tgtttaccta gatacttttg ttgtactggg cagccttggc gggcagcttg acgtcgaacc
    23821 aaaacgttgg tttgcgctcg ggacaattag cgcctctttc ctgtggttct ttggtctggc
    23881 tattctcgca gcctggctgg caccgcgcct gcgcacggca aaatcacagc gcattatcaa
    23941 tctggtagtg ggatgtgtca tgtggtttat agccttgcag ctggcgagag acggtattgc
    24001 tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta
    24061 agcttgcctc cagagatcct gaattttggg caagtcaatt aacacggaga gactaacgtg
    24121 aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct
    24181 aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagtgt ggatgcggtg
    24241 ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctacagcc
    24301 aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgaact caatcagatc
    24361 gcgaaaaaag atatcagctc agcgaattta cgcacccagc cagattatga ttatcaggat
    24421 ggtaaaagta tcctgaaagg ctaccgcgct gtgagaacgg tggaagtcac gctccgtcag
    24481 ttagacaaac tgaattcctt gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt
    24541 tcagtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg
    24601 attgataacg cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg
    24661 ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg
    24721 atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt acgagcaggc cgctattcag
    24781 tttgatgatc aggtcgatgt ggtcttccag ttagaacctg tggatcaaca acccgctaaa
    24841 acacctgcag cacaataata cttgtagccc gacgtattcg tcgggctaat gcctttaccc
    24901 gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc
    24961 tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga tgcggggagg
    25021 ggggatggac atttttattt cgaaaaagat gcgcaatttc attttattag cgcaaaccaa
    25081 taatattgcc cgggcggcag aaaaaatcca tatgacggct tcgccatttg gtaaaagcat
    25141 tgccgcgctg gaagaacaaa ttggctatac gctatttacc cgcaaagata ataatatcag
    25201 cctcaataaa gccggtcagg aactgtatca aaaactgttc ccggtttatc aacgactttc
    25261 tgcgattgat aatgaaatcc ataactcagg gcgtcgttca cgagaaattg tgattggcat
    25321 agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag ataagtatga
    25381 aggcgtgacc gcccagccag ttgagttcag cgaaaacggc gttatcgata atctttttga
    25441 ccgtcagtta gattttatta tctccccaca gcatgtatct gcacgagttc aggaactgga
    25501 aaacctgact atcagtgaat taccaccgct gcgattgaac tttctcgttt ctcgccgtta
    25561 tgaagagcgt caagagcaag agctgcttca ggagctgccc tagctacaaa tgcgctttca
    25621 aaaccgggct aacttcgaag caatgattga tgcgaatatg cgaccttgtg ggatcaatcc
    25681 caccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg agcgcggaca
    25741 ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa cgctgaaata
    25801 ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat ctttattcaa tcagaaatca
    25861 tcgttatacc gccacgatgc ttcagcatat tgctgaagat cgtgacggga caaaccatta
    25921 acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc
    25981 gtggtgaata tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat
    26041 aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag
    26101 atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgttgt gatcaacatg
    26161 gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa
    26221 gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc
    26281 gaccccggct acgtgcgttg agttggcatg cccgtaaata tcaaactcca ggccgacgtt
    26341 cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg
    26401 caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc
    26461 cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac
    26521 caccgattcc tgtagcactt ccgaatacat cataaacgga ggaatttccg ggttttcccc
    26581 cagacgcgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc
    26641 cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat
    26701 ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc
    26761 cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat
    26821 tgcatggaag atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatccgc
    26881 cagttctgca acgcgcggat cgtgatagtg attgagttca atgatcactt tcttcgctcg
    26941 cagcagccag gtcggcgcat taccgatccc gctggttaac cagactcgac catctggtgc
    27001 cagtgccgat gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat
    27061 ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt
    27121 acgtaaaccg gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag
    27181 tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt
    27241 tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gtgggtaggg ctttcggcga
    27301 acccgctggg gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc
    27361 ttcatcggcg gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat
    27421 taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa
    27481 tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc
    27541 atggtgtgtg cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc
    27601 gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc
    27661 acaacatggt tgaggatgcc gacagccagc gcgcgctggg cggtgatggg cgaagcggta
    27721 aaaatcagct ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc
    27781 aggttatacg ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg
    27841 ataatcagat cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc
    27901 atcgaaataa tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca
    27961 tcataggaga gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag
    28021 aagactttgg atccactcgg cgcgcgcaaa atgatacagc gaatttccgg ccggttgaga
    28081 tcgcttaacg cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgg
    28141 ccatagttaa actcaatgac cgccactttg ttgatagtga caacgttaac atactgataa
    28201 gacataaaaa ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag
    28261 gccggtgcgc ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg
    28321 atcgaaatct tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag
    28381 ccattccacc gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt
    28441 tttgaagtcg atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca
    28501 agtcagaacc cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc
    28561 gtacatatgc cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac
    28621 gatcagatca gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc
    28681 ggcaatttgc aacgagataa aacagtccac catgcgggcg acttctgttt ccgactgccc
    28741 gacgccaacc gtttcgacaa tcactacgtc ataacccgct gcttcgcata acagcattaa
    28801 ttcccgcgct cgctgactgg caccgcccag atgaccggag gatggtaccg ggcgaataaa
    28861 cgccgcttcg gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc
    28921 agtgaccggg ctgctgggat cgaccgcaat aaccgcgacc tttaatccct ctcgaatcaa
    28981 caacatgcca aaggcctcaa gaaaggtact tttccccgcg ccgggggtgc cggtaacgcc
    29041 cagtcgcagg gtgttaccgc agtacggcat aatggcatca agcagctgcg tacttagtgc
    29101 ctgatgacgc gggtgacggc tttccaccag cgtcatggcc tgggcgagtg tggcatgctc
    29161 accctgacgt aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc
    29221 ttatcagatt cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg
    29281 ccgccacgcc gcgctcttgc aggaaggcgt aatcctgcgg tggaatgacg ccaccggcga
    29341 ctacgcagat atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg
    29401 ttttatgacc ggcagccagt gaggatgcgc ccactacgtg aacgtcattt tctactgcca
    29461 ggcgggcgat ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat
    29521 cggaataggc gctggcgatc actttcgcgc cgcgatcgtg tccatccagg cccattttgg
    29581 cgatcagaat gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa
    29641 tggcatcgaa ctcggaggcc gatttctcag attgatgata gctttgcgca atcacgccgg
    29701 taacacactg gcttggcacc agataacggt cgaaagcggc ttccagcgca tcggaaattt
    29761 cacccagggt ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat
    29821 gctgtgcggc gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg
    29881 cgcgaatgcg ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa
    29941 gtacatcggt ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct
    30001 tgccctggtc gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca
    30061 gacctgcttc gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc
    30121 tggcttgttt gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat
    30181 cgacggtgcg gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc
    30241 gtgctgagaa atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact
    30301 gagtaccgcc cagcgtcgca gccagcgctt caatggtggt gcggataacg ttgttatacg
    30361 gatcctgttc agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt
    30421 tcgggtcctg tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca
    30481 acatggcgac gttcataaac agatccatgc cgatgccgaa gaagaacgac aggcgaggag
    30541 cgaagtcatc aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcag
    30601 cgagcgtaaa tgctacctgc tgcacgcagt tggcacccgc ttcacccatg tggtaaccgc
    30661 tgatactgat ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga
    30721 taatgcgcat tgacggtttt ggcgggtaaa tataggtgtt gcggcagagg tactctttga
    30781 gaatatcgtt ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttccgcgg
    30841 cgatgatata aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca
    30901 ttttatccag cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga
    30961 cgcccgtttt gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg
    31021 tggcaaggtc aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa
    31081 aagcgttgga ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg
    31141 cggtatacat agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg
    31201 tcacctccag attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg
    31261 cggtttgctg aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa
    31321 gctgttgcca ctcctgctcg ttagacatta tttgctccgt aaaacgcaat caaaatgagg
    31381 gtatttgcgc ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc
    31441 agacgtcgtt ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa
    31501 aaagaagtga ggataacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg
    31561 acgaaggact ttgtgaccat aatcgagcaa cgcatcagtg actttacgca tcatgcggct
    31621 ttcaggagca aagcggtgcc agtagagcat ccggcgttga aatagccctg gcgttaagtc
    31681 aatcagttca ccgctggcta gttctttctc gatttgcagg tgcgggatca tacagcaggt
    31741 ggtgccctgg cgagcaagtt gcacgaacgc ttctgaagaa ttaacgatat ggcagggcac
    31801 gctgcctgga ggcagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg
    31861 gtcaaacgcg accactggcg ctttcagtaa tgccgaacgc gttacgccgt tagggaaata
    31921 tttttcggca aagggttttg agctgacgaa cagatagtcg agcgcaccaa gtttatcgac
    31981 aagacaactc ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcgacg
    32041 cagacgttcc tgagtgcggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc
    32101 agccaataca ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cattgaccgc
    32161 cagtgaaagc agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag
    32221 caactccacc tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg
    32281 tacggtacgc accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga
    32341 gacggctgat tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat
    32401 caccgcatcc agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc
    32461 ctggtaaatg ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgcag
    32521 ccgtcggcgg gtaacatttg tatcaatggt taaacgcgca gcttaaaaga tcagggattt
    32581 gcgctattgc atttcgctta gcaacttcgc gccctggtta tgttgaatgg cgtggcgtta
    32641 ttgcctctgt tcgcaccgtg gagcggcata cccagaccgt tgtatgcttt atttgcgggg
    32701 cgaaagggga tgcccgccat tgcgcgatat tttatggatg agttaaccac gcggcttgcc
    32761 aacggggtct gaatcgcttt ttttgtatat aatgcgtgtg aaatttcata ccacaggcga
    32821 aacgatcatg acgcaggatg aattgaaaaa agcagtagga tgggcggcac ttcagtatgt
    32881 tcagcccggc accattgttg gtgtaggtac aggttccacc gccgcacact ttattgacgc
    32941 gctcggtaca atgaaaggcc agattgaagg ggccgtttcc agttcagatg cttccactga
    33001 aaaactgaaa agcctcggca ttcacgtttt tgatctcaac gaagtcgaca gccttggcat
    33061 ctacgttgat ggcgcagatg aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc
    33121 ggcgctgacc cgtgaaaaaa tcattgcttc ggttgcagaa aaatttatct gtattgcaga
    33181 cgcttccaag caggttgata ttctgggtaa attcccgctg ccagtagaag ttatcccgat
    33241 ggcacgtagt gcagtggcgc gccagctggt gaaactgggc ggtcgtccgg aataccgtca
    33301 gggcgtagtg accgataacg gcaacgtgat cctcgacgtc cacggcatgg aaatccttga
    33361 cccgatagcg atggaaaacg ccataaatgc gattcctggc gtggtgactg ttggcttgtt
    33421 tgctaaccgt ggcgcggacg ttgcgctgat tggcacacct gacggtgtca aaaccattgt
    33481 gaaatgatct gacgggggaa cctcccccgt taaaaaaatt ctcttcatta aatttggtga
    33541 catgtgtcac gtttttacct ggatattgtc gattgctcta aataaatcct ctaaaccagc
    33601 atattcaacc aagaaatacc tttgcgtgat atttcctcaa catcgcgacg caaacgttca
    33661 tattgccgca atattatttt ttgatatgtt gaaaggcgga tgcaaatccg cacacaacat
    33721 ttcaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt
    33781 ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc
    33841 aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat
    33901 gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca
    33961 gaaaagctgg tcgctattgg ctgtttctgt ataggaacaa accaggttga tctggatgcg
    34021 gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg
    34081 gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa
    34141 gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag
    34201 ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg
    34261 ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag
    34321 gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag
    34381 aatccgtcca ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa gcccggctcg
    34441 ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg tgatgcgctg
    34501 gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat
    34561 agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac gccacacatt
    34621 ggcggttcga ctcaggaagc gcaggagaat atcggcctgg aagttgcggg taaattgatc
    34681 aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt ctcgctgcca
    34741 ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg
    34801 ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct acaaacttcc
    34861 gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc cgaaaaagcg
    34921 ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta ctaattcccc
    34981 ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc ttctccacac
    35041 cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga
    35101 tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg gggctgtaac
    35161 ggtgaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg acttgtcatc
    35221 atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt ctcaggggag
    35281 gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg atgtcaatca
    35341 gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc atcgcccacg
    35401 tttactgccg taattaatcc cttattcctg caatgtatgt ctgccttaca tgacgtttta
    35461 cgagtgcgct cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga
    35521 gtttacgatg gcagggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    35581 tgaaggagaa gggttatgat gcgacttgtc atcatactga ttgtactgtt actgataagt
    35641 ttgccagctt attaacagtc agtctcaggg gaggagtcat cctcccttac ccttactcac
    35701 taaattaggt taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc
    35761 actcccagac tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg
    35821 ggagtttttc caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct
    35881 gccagttttg taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact
    35941 catcaaaggc gaccagcggt gtgatcagca cgtctaatcg ggaaaggggg agcacgtcac
    36001 gcacatccag ttttggctca tggattttca acctgttcat caccagttcg ctttgcggat
    36061 ggtaattcag gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc
    36121 gcttaccggc gcgccagagt tgttctataa gcggctgggt gtcgagttca ccatcaaaag
    36181 agaggaatac agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag
    36241 cggcttgttg acccatttcc tgctgttgtt ccggcgttaa cgcacgacga cgttgccgaa
    36301 tcattttgcg gatttcttgt cgggataatg tcagtgggag ttctggtagt tgcgtcatgg
    36361 ctggtgccag ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc
    36421 ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacggac cgagcatgct
    36481 caccaaccgc ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg
    36541 cttgcgaaca tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa
    36601 gtgtcattca aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg
    36661 ttctatggtc tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt
    36721 cttcgctttt tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg
    36781 ttcagtattt gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc
    36841 cgctgcctga ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc
    36901 aaaaatttgg atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct
    36961 gccttcgtct ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa
    37021 gagacaagcc cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct
    37081 ccctttgacg acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga
    37141 accagtatct gaaccaacaa gggacgggtc tgacccctgc tgagatgcat ggtttaatca
    37201 gtgggatgat atgtggcggt aacgatgaca gctcatggct accgctactt cacgacctga
    37261 cgaacgaagg catggctttc ggtcatgagc tggcacaggc attgcgtaaa atgcactctg
    37321 ccaccagcga tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg
    37381 atgatgtcag cgttttcgat cgggctgatg cattggcagg ttgggtcaat cacttcctgc
    37441 ttggtcttgg tgttacgcaa ccgaagctgg ataaagtgac cggcgaaacc ggtgaagcta
    37501 tcgacgatct gcgtaacatt gcgcaactgg gttacgacga agacgaagat caggaagagc
    37561 ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg
    37621 acacctttac tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa
    37681 aacaaaaaac gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc
    37741 cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca
    37801 gaagtaacac gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac
    37861 ttcaccggct ttaacgaacc ggaagcggtg ttggtgctga ttaaaagcga tgacactcat
    37921 aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt
    37981 cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc
    38041 gaaattaatc agcaacttta tcaactactt aatggtctgg atgtggttta ccatgctcag
    38101 ggcgaatatg catatgctga tgaaatcgtg aacagtgcgc tggaaaaact gcgtaaaggt
    38161 tcgcggcaaa atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa
    38221 atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc
    38281 gccatggcac atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg
    38341 gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc gctatccgtc ctataacacc
    38401 attgtaggca gcggtgaaaa cggctgcatt ttacactaca ccgaaaacga gtgtgaaatg
    38461 cgcgacggcg acctggtgtt gattgacgca ggctgcgaat acaaaggtta cgcaggcgat
    38521 atcacccgta ccttcccggt caacggcaaa ttcacccagg cccagcgcga aatctacgac
    38581 attgtgctgg agtctcttga aaccagcctg cgcctgtatc gtccgggaac ctccattctg
    38641 gaagtcaccg gtgaagtggt gcgcattatg gttagcggcc tggtaaaact cggcatcctg
    38701 aaaggtgata ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc
    38761 cttagccact ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg
    38821 cgcattctgg aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat
    38881 gcggatgtgc cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt
    38941 accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa
    39001 gcgttgatgg ctgctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg
    39061 gcgcgacgct ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga
    39121 ttgaagcgac tgcgccagag tcacatgctc atccgggctt tgatggacga gcgatagcgc
    39181 tggcggcggg tacctgtcag caactggcgc gcatcggcgt ttggcaatct ctggcgcatt
    39241 gcgcaactgc catcaccacc gtgcatgtca gcgatcgagg tcacgctgga tttgtcaccc
    39301 tcgccgcaga agattaccaa ctggcggcgc tgggacaggt tgtcgaattg cacaatgtcg
    39361 ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt aacgctgcat tgccctgatc
    39421 gcgtggctaa cgttgcccgt actcagagtc acgttgaagt gacgctggag ggtggcgaga
    39481 cgctgacggg ccgcgtgctg gtagccgctg atggcaccca ttcagcgtta gccactgtgt
    39541 gcggcgttga ctggcagcag gagccttacg aacaactggc tgtgattgcc aacgttgcga
    39601 cttccgttgc gcatgaaggg cgcgcttttg aacgctttac gcaacatggc ccgctggcga
    39661 tgttgccgat gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg
    39721 aagaggtgct gtcgtggagc gacgagaagt tttgccgtga actccagtcg gcctttggct
    39781 ggcgacttgg gaaaattacc cacgctggta aacgcagtgc ttatccgctg gcgttaaccc
    39841 acgccgccag atctattacc catcgtaccg tgctggtggg caatgcggcg caaaccctgc
    39901 acccgattgc cgggcaaggg ttcaacctcg gtatgcgaga tgtcatgagt cttgcggaaa
    39961 ccctgactca ggcgcaggag cgcggagaag acatgggtga ttacggcgta ttgtgtcgtt
    40021 atcagcagcg tcgacagagc gatcgcgaag caaccattgg cgtcacggac agccttgtac
    40081 atctttttgc caaccgttgg acgccactgg ttgtcgggcg caacatcggg ctgatgacga
    40141 tggaattatt caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc
    40201 gttgaaggcg gttattaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg
    40261 cggcatggtg gggctggcgg ttgcctgcgg cttacagggt agcggcttac gcgttgccgt
    40321 actggagcag cgcgtaccgg aacctctggc ggcggatgca ccaccacaac tgcgcgtttc
    40381 ggctatcaat gccgccagcg aaaaattact cacccgtctt ggcgtctggc aggacattct
    40441 ctctcgccga gccagctgtt atcacggtat ggaagtgtgg gacaaagaca gttttggtca
    40501 catctcgttt gacgatcaaa gcatgggcta tagccatctt gggcacatcg ttgaaaactc
    40561 agtgattcac tacgcgctgt ggaacaaagc gcagcagtcg tcagatatca ctcttttggc
    40621 ccccgcagaa ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgctgaaaga
    40681 tggcagtatg ttaacggcgc gtctggtgat tggcgcggac ggcgctaatt cctggttgcg
    40741 caacaaagcc gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac
    40801 catacgcacg gaagaaccgc atgatgcggt ggcgcggcag gttttccatg gcgaaggcat
    40861 tctggccttt ttaccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc
    40921 agaggaagcg cagcggatgc agcaggcaag tgaagacgaa tttaatcgcg cgttaaatat
    40981 cgcttttgat aatcgcctgg gcttatgcaa ggttgagagc gcgcgtcagg tgttcccact
    41041 gacggggcgt tatgcgcgcc agtttgccgc gcaccgtctg gcgctggtgg gcgacgccgc
    41101 gcataccatt cacccgctgg cggggcaggg ggtaaatctt ggctttatgg atgctgcaga
    41161 gctgattgct gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta
    41221 tctgcgtcgc tatgagcgta gccgcaagca cagtgcggcg ctgatgctgg ctggtatgca
    41281 gggattccgc gatctgtttt ctggtaccaa tccggtgaaa aaactgctgc gtgatattgg
    41341 tctgaaactg gccgacacgc ttcctggcgt taaaccacaa cttatccgcc aggcaatggg
    41401 attaaacgat ttgcctgaat ggctgcgtta aaaatttctc ttctgctgtt tatttgatac
    41461 ccatcacact ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact
    41521 aatttcacct ccgttttcgc attatatttt ctaatgtcac gaatttttga tttagtgttt
    41581 tttgacattt tttcagctct taatattgcc ttattcaaat taactttctc atcacatcat
    41641 ctttatatag aaacttgtgt attttttggt tttttattct gttgcgattt tggcgttttt
    41701 taaccataag ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt
    41761 aagttcaggc aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt
    41821 cgtggtgata atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat
    41881 ggcacaacag actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt
    41941 ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgaggtacg
    42001 taccgatgcc ggaatgtttg atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg
    42061 cacccgggag tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg
    42121 caaagccctt tactcgggga tgttgaatgc ctctggcggt gtgatagatg acctcatcgt
    42181 ctactacttt actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga
    42241 cctctcctgg attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga
    42301 cctttccatg atcgccgtac aagggccgaa tgcgcaggca aaagctgcca cactgtttaa
    42361 tgatgctcag cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggcgggcga
    42421 tctgtttatt gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa
    42481 tgaaaaagcg gccgatttct ggcgtgcgct ggtggaaggg ggcgttaagc catgcggtct
    42541 gggcgcgcgt gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga
    42601 cgaaaccatc tctccgttag ccgccaacat gggctggact atcgcctggg aaccggcaga
    42661 tcgtgacttc atcggtcgtg aagccctgga agtgcagcgt gagcatggca cagaaaaact
    42721 ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgttttac
    42781 cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc gggactttct ccccgacgct
    42841 gggttacagc attgcgctgg cacgcgtgcc gaaaggtatt ggcgaaacgg cgattgtgca
    42901 aattcgcaac cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa
    42961 agccgtcgcg tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg
    43021 aaatacagca aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt
    43081 attaccgaac atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg
    43141 ggcgcaacgg ttagcgcggg cgatgactgc gcggttgccg aatcagtaaa agcggcgtca
    43201 gacatttatg cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgactcc
    43261 ccggaactgg tgaacagcga accgtatgca ggtggctgga tctttaaaat caaagccagc
    43321 gatgaaagcg aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagac
    43381 gagtaacggc tttattcctc ttctgcggga gaggatcagg gtgaggaaat ttacacctca
    43441 ccctcactct cttcgcaagg agagaggttc acaattcact gcacgtttca ggaaccatcg
    43501 ctcatgacac agacgttaag ccagcttgaa aatagcggcg cttttattga acgccatatc
    43561 ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg ttggcgcaca atcgttaaac
    43621 gcgctgaccg gccagattgt accgaaagat attcagcttg cgaccccgcc gcaggttggc
    43681 gcaccggcga ccgaatacgc cgcgctggcg gaactcaagg ctattgccag tcgcaataaa
    43741 cgcttcacgt cttacatcgg catgggttac accgccgtgc agttaccgcc ggttatcctg
    43801 cgtaacatgc tggaaaatcc gggctggtat accgcgtata ctccgtatca acctgaagtc
    43861 tcgcagggcc gccttgaggc actgctcaat ttccagcagg taacgctgga tttaaccggg
    43921 ctggatatgg cctctgcttc tcttcttgac gaagccaccg ctgccgccga agcaatggcg
    43981 atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccgatgtg
    44041 catccgcaaa cgctggatgt ggtccgtact cgtgccgaaa cctttggttt tgaagtgatt
    44101 gtcgatgatg cgcaaaaagt gctcgaccat caggacatct tcggcgtgct gttacagcag
    44161 gttggcacta ccggtgaaat tcacgactac actgcgctta tcagcgaact gaaatcacgc
    44221 aaaattgtgg tcagcgttgc cgctgatatt atggcgctgg tgctgttaac tgcgccgggt
    44281 aaacagggcg cggatattgt ttttggttcg gcgcaacgct ttggcgtacc gatgggttac
    44341 ggtggcccac acgcggcatt ctttgcggcg aaagacgaat acaaacgctc aatgccaggc
    44401 cgtattatcg gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag
    44461 actcgcgagc aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttctcaggtg
    44521 ctgctggcaa acatcgccag cctgtatgcc gtttatcacg gcccgattgg cctgaaacgt
    44581 atcgctaacc gcattcaccg tctgaccgat atcctggcgg caggcctgca acaaaaaggt
    44641 ctgaagctgc gccatgcgca ctatttcgac actctgtgtg tggaagtggc cgacaaggcg
    44701 ggcgtactgg cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcg
    44761 gttgggatca cccttgatga aaccaccacg cgcgaaaacg tgatgcagct tttcagcgta
    44821 ctactggggg ataaccacgg gctggacatc gacacgctgg acaaagacgt ggctcacgac
    44881 agccgctcta tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt
    44941 aatcgctacc acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat
    45001 ctggcgctga atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc
    45061 gccgagatga tcccaatcac ctggccggaa tttgccgaac tgcacccgtt ctgcccgccg
    45121 gagcaggccg aaggttatca gcagatgatc gcgcagctgg ctgactggct ggtgaaactg
    45181 accggttacg acgccgtttg tatgcagccg aactctggcg cacagggcga atacgcgggc
    45241 ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg
    45301 atcccggctt ctgcgcacgg cactaacccc gcttctgcac atatggcagg aatgcaggtg
    45361 gtggttgtgg cgtgtgataa aaacggaaat atcgatctga ctgatctgcg cgcgaaagcc
    45421 gaacaggcag gtgataatct ctcctgcatc atggtgacct atccttccac ccacggtgtg
    45481 tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac
    45541 cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgca
    45601 gacgtttcac accttaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg
    45661 ggtatgggac cgatcggcgt gaaagcgcat ctggctccgt ttgtaccggg tcatagcgtg
    45721 gtgcaaatcg aaggcatgtt aacccgtcag ggcgcggttt ctgcggcacc gttcggtagc
    45781 gcctctatcc tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa
    45841 aaagcaagcc aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc
    45901 ttcccggtgc tgtataccgg tcgtgacggt cgcgtggcgc acgaatgtat tctcgatatt
    45961 cgtccgttga aagaagaaac cggcatcagc gagctggata ttgccaagcg cctgatcgac
    46021 tacggtttcc acgcgccgac gatgtcgttc ccggtggcag gtacgctgat ggttgaaccg
    46081 actgaatctg aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc
    46141 gcagaaattg accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac
    46201 gcgccgcaca ttcagagtga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt
    46261 gcggtattcc cggcaggcgt ggcagacaaa tactggccga cagtgaaacg tttggatgat
    46321 gtttacggcg accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc
    46381 actgattcga ctatcttcta aaggcgcttc ggcgcctttt tagattgtta ataaagtgcg
    46441 ctttgtgtgt ttcggctgag gcgtaatata aaatattgcg agctcaatat attaaaagag
    46501 tgccaggcct gatgagcgta gcgcatcagg cacatcggct aattacagat tctcgccgtt
    46561 gctggcaatc acttctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc
    46621 agtaccgtcg tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt
    46681 agtgaacgac acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacgccatc
    46741 gtaagttact gcttttttca tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc
    46801 gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat
    46861 aaacagcggc ttctggtaac gctcatacag ttcgcaaagt gcgtaacgca gacccaccgg
    46921 atcaatctgc cagccccagt cagatgcttt aacatacggg tttggtacgc tgccttcaaa
    46981 accagagatc gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct
    47041 gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg agatcgctat cttccatttt
    47101 gatgttaaat ccgcgacgct cccactcgtt caacacatac gacgggtaat agccgcgtag
    47161 ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc
    47221 cgggttacat gagtagggat agagcggcac catcgccagc atacagccga ctttcatctc
    47281 caggttaatg cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact ggtgatgcag
    47341 cacctgatac atcgtctctt ccgggttttc atgctcggta tacaccacgc cggagcagca
    47401 gtaaccgaac agcggtgcac gccagttacg ctggttgtta atttcgttga aggtcatcca
    47461 gtatttgact ttgtgcttat agcgttcaaa taccacttcc gcgaaacgta caaagaaatc
    47521 aaccacttta cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa
    47581 gtgggagagg gtgatcaccg gttcgatgtt gtatttcagc agttcatcga acagagagtc
    47641 gtagaacttc agcccttctt cgtttggctg agcttcatcg ccttttggaa aaatgcgcgt
    47701 ccaggcaatg gatgtacgaa aacatttgaa gcccatttcg gcaaatagct tgatgtcttc
    47761 cttatagtga ccataaaaat caacggcttc atggtttgga tagtatttgc ctggcaagac
    47821 ttctttggtt atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct
    47881 cggccctttt ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa ctgcgccgcc
    47941 ccataagaaa tctttcggta aggtgagttt tttcactatc ataggctcca gatgagtcaa
    48001 ttcttacagg aaatagtacc atgcagccaa acgacatcac tttttttcaa cgtttccagg
    48061 atgacattct ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca
    48121 aaacgggtga tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg
    48181 aagtcaccgc aacctcaacc gtaacgctgg atacgctgac ggaaaaacat gcagagcagg
    48241 aaaatatgac cctgactgaa ctgaaaaagg tcattgccga catctatccc ggccagacac
    48301 agttttatgt gattgaattt aaatgccttt aaattgaact cacgcctgtt agttgaaatt
    48361 tcgcatttaa tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa
    48421 caggtgttca ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt
    48481 tctccggagt aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag
    48541 gaaattgcca acagcgtcag tcacggcatt gggttggtgt ttggtatcgt tgggctggtg
    48601 ttgctactgg ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagctacagc
    48661 ctctatggcg gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct
    48721 catcaacggg caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt
    48781 gccggaacct acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg
    48841 atgattgtta tctggagcct ggcattgctg gggattctgt tcaaactgac catcgcgcac
    48901 cgattcaaaa ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta
    48961 atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc
    49021 gtggtttatt cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc
    49081 atctggcacg gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat
    49141 attgggcagg cgtaatgggt atgcctttgt aggcctgata agacgcgcca gcgtcgcatc
    49201 aggcgataaa tgcctgatgc gacacagaga tttactcttc gagcgaatac ggcagtggct
    49261 cgatacacaa tgtattcgca tcgtcgcgca cgcggaagat gctgtccggt tccatatcgt
    49321 tattcatcac tacctgcacc acgacctgac catcttccag ttttaccgca gccagcaccg
    49381 taccagtacg acgccagttc tcgcccattt tcagctctaa gtcttcacca gcttccggca
    49441 gtcggctggc actacctgtc agcaaccaga gcgcgcgttt gttggcacca cggaattttg
    49501 ctcgcgccac catctcttgt ccggtatagc agcctttctt aaagctgata ccgcccagcg
    49561 cctggaggtt ggtcgcctgt gggataaact gcccgctgtt cgcggcatca atcaccggga
    49621 aacccgcttc aatgtttaat gccagccact gttggctatt gttcagttcc gcttcaccgc
    49681 gcagtttatc ggtcagcata ttggcagtag cttcatcggt tacgatcagg aaacgttctg
    49741 ccgggtgttc aaaccatagc aaagtggtcg cgccttcttt aactacctgt ttttctttcg
    49801 aaggcagttc actaaagata tttgccagcg cggcgcgcgc ctgaaaaccg gcaacaccaa
    49861 gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga gaacaccgca tattttttca
    49921 gttcagccag ctgcggttca cgcacgctgc gccgttcaat ccatgcaaag ccatcgccgt
    49981 cgcggaacag acgtaaattg ctccacattt tacctttggc gtcgcaatgg gcggcgagca
    50041 ggtgctgatc atctgtcatc tggctgacat ctgctgtcac ctggccctgc aaatattttt
    50101 cgctgtccgc gccagtaatg gtggcaagcg cccagtcatc aagcgtcatc agcgtcagtg
    50161 gcaaacgggc agaagccgta ggctgacggg gaggaaaagg tgtaaaagcc ataatagagt
    50221 cctgagttgc ttaacggcgt attaataact aatggtaaaa gagctatgag gcaatgcaag
    50281 tgctttcact acgcgatctg tgccttctgc gaggcgactt cccaaaaaat gaagccctta
    50341 aattgttgtg aattaatttc gcgatcgcga ttgtgtttac tgatattccc gtaaaagaag
    50401 tcgaaaaaca cgttacaata gccgggtact ggattttcgt gagacaggaa gaagatggac
    50461 attaacaaca aagcccgcat tcattgggca tgccgccgtg gtatgcgcga actcgatatt
    50521 tcaatcatgc cgtttttcga acatgagtat gacagcttaa gcgatgacga aaaacgcatc
    50581 tttattcgtc tgctggaatg tgacgatccc gatctgttta actggctgat gaatcacggt
    50641 aaaccggccg atgcagaact ggaaatgatg gtccgactca tccagacacg gaaccgggaa
    50701 cgtggtcctg tggcaatctg atttgcgcgt ctcctggcgc gcacagtggc tttccttgct
    50761 gattcatggg ctggttgccg ctgttatttt actcatgccc tggccgctca gttacacccc
    50821 gttatggatg gtgttacttt cgctggtggt gtttgattgc gttcgcagcc agcggcgtat
    50881 taatgctcgc cagggggaaa ttcgcttgtt gatggacggg cgtttgcgtt ggcaagggca
    50941 ggagtggagc atcgtcaaag cgccgtggat gattaagagc ggcatgatgc tgcgtttacg
    51001 ttctgatagc ggtaaacggc aacatttatg gctggcagcc gacagcatgg acgaagccga
    51061 atggcgggat ttacggcgga ttttgttgca acaagagacg caaagataaa gatgacggca
    51121 aagcatatgc gctaagccgt cagtgatatc aggcgtaatg ctctgccatt tcgttgagga
    51181 tttgctcgca ccagctctga atacgctcgt cgctaaggtc atactggtta gtttcgtcca
    51241 gcgccagccc cacgaacagt tgcccgtcag caatcaccgg tttcgggctg gtaaattcat
    51301 acccttccgt tggccagtag ccgacgaact tcacgccttt ggtcgagagt ttgtcatgca
    51361 gcataccgag cgcatcgagg aaccactcgc cgtatcccag ttgatcgcca agcccataca
    51421 gcgcaacaat tttaccttcg aggttcaggt cgtcgagctg atcccagacg gcttcccagt
    51481 cttcctggat ttcgccaaaa tcccaggtcg ggatacccag aatgagcaca tcgtactgct
    51541 ccattaattt cggggagtcg tccttgaggt tatgtaaggt caccagttct gggccgataa
    51601 tatcgcggat tttttctgcc gccatttcgg tgtaacaggt gctggaaccg taaaaaagac
    51661 ccatattcat aacgtaagct tctcgattct gctgtggctt ttgcggctag tgtatcagaa
    51721 tcgctttagt cacggcacaa taaggcataa tggatcaaat gagtgaccat aaggggcgca
    51781 agtgaaacag gatctggcac gcatcgagca gtttcttgat gctctgtggc tggaaaaaaa
    51841 tctggctgaa aatacgttga acgcttaccg tcgcgatctg tcaatgatgg tggagtggtt
    51901 gcatcaccgc gggttgacgc tggcgacggc gcaaagtgac gatttacagg cattactggc
    51961 agaacggctg gagggcgggt ataaagccac cagctcagcg cgtttgctga gtgcagtgcg
    52021 ccgattgttc cagtatcttt atcgcgaaaa gtttcgtgaa gatgatccca gtgcgcatct
    52081 cgcttcaccg aaattgcccc agcgtttgcc aaaagattta agcgaagcgc aggtcgaacg
    52141 tttattacag gcaccattaa ttgatcagcc actggagcta cgcgataaag ctatgcttga
    52201 agtgttgtat gctaccgggc tgcgtgtctc tgaactggtc gggctgacaa tgagtgatat
    52261 cagcctgcgt cagggcgtag tgcgggtcat tggtaaaggc aacaaagagc gcctggtgcc
    52321 gttaggtgaa gaggcggttt actggctgga aacctatctg gaacatgggc gtccgtggct
    52381 actgaatggt gtgtcaattg acgtgttgtt tcccagccag cgtgcgcagc agatgacgcg
    52441 acagaccttc tggcaccgca ttaaacatta tgccgtgctg gcgggtatcg acagcgaaaa
    52501 gctgtcaccg catgtgttgc gtcacgcttt tgccactcac ttattaaatc atggtgcgga
    52561 tttacgcgtg gtgcagatgc tactgggcca cagcgatctc tccaccacgc aaatttatac
    52621 gcatgtcgct accgagcgtc tgcggcaact tcatcaacag catcacccgc gggcgtgatg
    52681 tctgaaaaga acgggaagat ttatgaagaa aggttttatg ttgtttactt tgttagcggc
    52741 gttttcaggc ttcgctcagg ctgatgacgc ggcaattcaa caaacattag ccaaaatggg
    52801 catcaaaagc agcgatattc agcccgcgcc cgtagctggc atgaagactg tgctgactaa
    52861 cagcggtgtg ttgtacatca ccgatgatgg taaacatatc attcaggggc caatgtatga
    52921 cgttagcggc acggctccgg tcaatgtcac caataagatg ctgttaaagc agttgaatgc
    52981 gcttgaaaaa gagatgatcg tttataaagc accgcaggaa aaacacgtca tcaccgtgtt
    53041 tactgatatt acctgtggtt actgccacaa actgcatgaa caaatggcag actataacgc
    53101 gctggggatc accgtgcgtt atcttgcttt cccacgccag gggctggaca gcgatgctga
    53161 gaaagaaatg aaagctatct ggtgtgcgaa agataaaaac aaagcgtttg atgatgtgat
    53221 ggcaggtaaa agcgtcgcac cagccagctg cgacgtggat attgccgacc attacgcact
    53281 tggcgtccag cttggcgtta gcggtactcc ggcagttgtg ctgagcaatg gcacacttgt
    53341 tccgggttac cagccgccga aagagatgaa agaattcctc gacgaacacc aaaaaatgac
    53401 cagcggtaaa taattcgcgt gaaacaacag atacaacttc gtcgccgtga agtcgatgaa
    53461 acggcagact tgcccgcgga attgcctccc ttgctgcgcc gtttatacgc cagccgggga
    53521 gtacgcagtg cgcaagaact ggaacgcagt gttaaaggta tgctgccctg gcagcaactg
    53581 agcggcgtcg aaaaggccgt tgagatcctt tacaacgctt ttcgcgaagg aacgcggatt
    53641 attgtggtcg gtgatttcga cgccgacggt gccaccagca cggctctaag cgtgctggcg
    53701 atgcgctcgc ttggttgcag caatatcgac tacctggtac caaaccgttt cgaagacggt
    53761 tacggcttaa gcccggaagt ggtcgatcag gcccatgccc gtggcgcgca gttaattgtc
    53821 acggtggata acggtatttc ctcccatgcg ggagttgagc acgctcgctc gttgggcatc
    53881 ccggttattg ttaccgatca ccatttgccg ggcgacacat tacccgcagc ggaagcgatc
    53941 attaacccta acttgcgcga ctgtaatttc ccgtcgaaat cactggcagg cgtgggtgtg
    54001 gcgttttatc tgatgctggc gctgcgcacc tttttgcgcg atcagggctg gtttgatgag
    54061 cgcggcatcg caattcctaa cctggcagaa ctgctggatc tggtcgcgct ggggacagtg
    54121 gcggacgtcg tgccgctgga cgctaataat cgcattctga cctggcaggg gatgagtcgc
    54181 atccgtgccg gaaagtgccg tccagggatt aaagcgctgc tggaagtggc aaaccgtgat
    54241 gcacaaaaac tcgccgccag cgatttaggt tttgcgctgg ggccacgtct caatgctgcc
    54301 gggcgactgg acgatatgtc cgtcggtgtg gcgctgttgc tgtgcgacaa catcggcgaa
    54361 gcgcgcgtgc tggcaaatga actcgatgcg ctaaatcaga cgcgaaaaga gatcgaacaa
    54421 ggaatgcaga ttgaagcctt gaccctgtgc gagaaactgg agcgaagtcg cgacacgcta
    54481 cccggcgggc tggcaatgta tcaccccgaa tggcatcagg gcgttgtcgg tattctggct
    54541 tcgcgcatca aagagcgttt tcaccgtccg gttatcgcct ttgcgccagc aggtgatggt
    54601 acgctgaaag gttccggtcg ctccattcag gggctgcata tgcgtgatgc gctggagcga
    54661 ttagacacac tctaccccgg tatgatgctg aagtttggcg gtcatgcgat ggcggcgggt
    54721 ttgtcgctgg aagaggataa attcgaactc tttcaacaac ggtttggcga gttggttacc
    54781 gagtggctgg acccttcgct attgcaaggc gaagtggtgt cagacggccc gttaagcccg
    54841 gccgaaatga ccatggaagt ggcgcagctg ctgcgcgatg ctggcccgtg ggggcagatg
    54901 ttcccggagc cgctgtttga tggtcatttc cgtctgctgc aacagcggct ggtgggcgaa
    54961 cgtcatttga aagtcatggt cgaaccggtc ggcggcggtc cgctgctgga tggtattgct
    55021 tttaatgtcg ataccgccct ctggccggat aacggcgtgc gcgaagtgca actggcttac
    55081 aagctcgata tcaacgagtt tcgcggcaac cgcagcctgc aaattatcat cgacaatatc
    55141 tggccaattt agcgtcatct tctctataaa aaagagcgtg gattgggtac aatcccgctc
    55201 ttatcaccgc attttgacta gctcaataaa agaaatcaga ccatgtttga aattaatccg
    55261 gtaaataatc gcattcagga cctcacggaa cgctccgacg ttcttagggg gtatctttga
    55321 ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc gagctggaac agccggatgt
    55381 ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag cgttcctccc tcgaagccgt
    55441 tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc tgctggaact
    55501 ggctgtagaa gctgacgacg aagaaacctt taacgaagcc gttgctgaac tcgacgccct
    55561 ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat atgacagcgc
    55621 cgactgctac ctcgatattc aggcggggtc tggcggtacg gaagcacagg actgggcgag
    55681 catgcttgag cgtatgtatt tgcgctgggc agaatcgcgt ggtttcaaaa ctgaaatcat
    55741 cgaagagtcg gaaggtgaag tggcgggtat taaatccgtg acgatcaaaa tctccggcga
    55801 ttacgcttac ggctggctgc gtacagaaac tggcgttcac cgcctggtgc gtaagagccc
    55861 gtttgactcc ggcggtcgtc gccacacgtc gttcagctcc gcgtttgttt atccggaagt
    55921 tgatgatgat attgatatcg aaatcaaccc ggcggatctg cgcattgacg tttatcgcgc
    55981 gtccggcgcg ggcggtcagc acgttaaccg taccgaatct gcggtgcgta ttacccacat
    56041 cccgaccggg atcgtgaccc agtgccagaa cgaccgttcc cagcacaaga acaaagacca
    56101 ggccatgaag cagatgaaag cgaagcttta tgaactggag atgcagaaga aaaatgccga
    56161 gaaacaggcg atggaagata acaaatccga catcggctgg ggcagccaga ttcgttctta
    56221 tgtccttgat gactcccgca ttaaagatct gcgcaccggg gttgaaaccc gcaatacgca
    56281 ggccgtgctg gacggcagcc tggatcaatt tatcgaagca agtttgaaag cagggttatg
    56341 aggaaccaac atgtctgaac aacacgcaca gggcgctgac gcggtagtcg atcttaacaa
    56401 tgaactgaaa acgcgtcgtg agaagctggc gaacctgcgt gagcagggaa ttgccttccc
    56461 gaacgatttc cgtcgcgatc atacctctga ccaattgcac gcagaattcg acggtaaaga
    56521 gaacgaagaa cttgaagcgc tgaacatcga agtcgccgtt gctggccgca tgatgacccg
    56581 ccgtattatg ggtaaagcct cttttgttac cctgcaggac gttggcggtc gtattcagct
    56641 gtacgttgcc cgtgacgatc ttccggaagg cgtttacaac gagcagttca aaaaatggga
    56701 cctcggcgac atcctcggcg cgaaaggtaa actgttcaaa accaaaaccg gcgaactgtc
    56761 tatccactgt accgagttgc gtctgctgac caaagcactg cgtccgctgc cggataaatt
    56821 ccacggcttg caggatcagg aagcgcgcta tcgtcagcgt tatctcgatc tcatctccaa
    56881 cgatgaatcc cgcaacacct ttaaagtgcg ctcgcagatc ctctctggta ttcgccagtt
    56941 catggtgaac cgcggcttta tggaagttga aacgccgatg atgcaggtga tccctggcgg
    57001 tgccgctgcg cgtccgttta tcacccacca taacgcgctg gatctcgaca tgtacctgcg
    57061 tatcgcgccg gaactgtacc tcaagcgtct ggtggttggt ggcttcgagc gtgtattcga
    57121 aatcaaccgt aacttccgta acgaaggtat ttccgtacgt cataacccag agttcaccat
    57181 gatggaactc tatatggctt acgcagatta caaagatctg atcgagctga ccgaatcgct
    57241 gttccgtact ctggcacagg atattctcgg taagacggaa gtgacctacg gcgacgtgac
    57301 gctggatttc ggtaaaccgt tcgaaaaact gaccatgcgt gaagcgatta agaaatatcg
    57361 cccggaaacc gacatggcgg atctggacaa cttcgactct gcaaaagcga ttgttgaatc
    57421 tatcggtatc cacgttgaga agagctgggg tctgggccgt atcgttaccg agatcttcga
    57481 agaagtggca gaagcacatc tgattcagcc gaccttcatt actgaatatc cggcagaagt
    57541 ttctccgctg gcgcgtcgta acgacgttaa cccggaaatc acagaccgct ttgagttctt
    57601 cattggtggt cgtgaaatcg gtaacggctt tagcgagctg aatgatgcgg aagatcaggc
    57661 gcaacgcttc ctggatcagg ttgccgcgaa agatgcaggt gacgacgaag cgatgttcta
    57721 cgacgaagat tacgtcaccg cactggaaca tggcttaccg ccgacagcag gtctggggat
    57781 tggtatcgac cgtatggtaa tgctgttcac caacagccat accatccgcg acgttattct
    57841 gttcccggcg atgcgtccgg tgaaataagc attacgttat gcgcataacc ccggcagatg
    57901 tcggggtttt ttaattgtgc tgcgcgaaag cagacaacaa ttttcttgct tcactattgg
    57961 ctgcctgcat caccatccac ggactgaacg cccacggcgt ggcatcaata ccgtgtaata
    58021 catctgctaa atcacaccat tgataatcca tcacttcatc atcgttgatc tgtaacgcac
    58081 tggtggtgcg tgcggcaaat accggacaca cttcattttc cacaatgcca ttcggatcgg
    58141 tggcgcgata gcgaaagtca ggatagatag attcaggagg cgtaatttcc acgccaagct
    58201 cataacggca acggcggatc accgcgtctt cgttgctttc tcccagttgt gggtgcccac
    58261 aaaccgagtt agtccacacg ccaggccatg cttttttgct cagtgcgcgg cgggtaacta
    58321 ataattgtcc tttggcatta aacagccaac tggagaacgc gagatgtaag cgggtgtctg
    58381 ccgtgtgtgc ggcatacttt tccagcgtac ccgtgggaac tccctgtgca ttcaataaaa
    58441 tgacgtgttc cgtttgcata atttctcaca tgtaattctg atcactcatc attatagata
    58501 attgaagagt gaaatgatta accaagcgag cgtttatcga caaccgcccg ttttgagcgg
    58561 atgccgacag gtaaaaacca tttaaaaaag tagcatcata tatttgaagc gactttcaat
    58621 ggccattgca acacagtcag aaatgacagg aatagagaga gtaaaaagca acagcagact
    58681 gaatatagcg cgcattaaaa aattcatatt attctccatg ctcatttttc ttcagcttgc
    58741 gcatcagaag ccacatacgt actgtcctga cttttaaatc tttatcagag acggattcct
    58801 tcaccaggtc agcatgatgc tgatagccat taaagaagac gttcagcaca acggcgctaa
    58861 gggttgcgag cataatgccg ctatgtagca gcggttgcag tacggccggt aacttagaaa
    58921 agaaatcgtg agagagcgtt ggagtcatgc caacgccgag actgatcgcc acaatataga
    58981 ggttgtaacg gttggtggtg tagttacagc gcgacagaat tcgaatccct gtcgccagta
    59041 ccatgccgaa catcaccaga ccagcgccgc ccagcacaaa ttgcggaatg gaggctacca
    59101 gcaccgccat ttttggcacc atgccaaaca ggattaatat aattcccgaa gaaatacaca
    59161 cccagcggct atgaacgcgc gtcacgctaa ccaggccaac gttttgagaa aatgacgtgt
    59221 gggggaagct gttaaacgtg ccaccaatca ttgtccctac gccatcgaca cgcagcccgc
    59281 gaataatatc gtgcgaagag agtttacgac cgactatttc acccagtgcc aggaacatcc
    59341 ccatcgactc gataaacacg atgattaaca cggcagtcat ggtcagaatg gaaacgggat
    59401 cgaaaatcgg cataccaaac gacatcgggg tgacaatcgc aaaccacgag gcatcatgta
    59461 gcccggataa attgacttca ttcatcatcc acgaaagtaa aaagccaaat acaatcccca
    59521 gtaatacggc gacgttggac ataaatcctt tcgcatagcg agtaatgagc aagataaaaa
    59581 ttaagacggc aaaggagata cctaaataaa cgggattacc atattgcgga ttccctttac
    59641 ctccggcggc ccagtcaata cccacctgaa tgatgctaag cccgatagaa gtaataacca
    59701 caccggtaac cagtggcggg aataaaggca tcaagcgacc gataagttgc gctaataatg
    59761 tggtgataaa acccgcggcg atagtggcac caaatatacc cagcaggccg atatccgggt
    59821 tcatcccaat ggctatcatt ggtgttacag cagcaaaggt caccgacata atcaccggca
    59881 ggcggatccc cataaagcgg ccgataccga tacattgcaa taatgtgacg atcccgcagc
    59941 aaaagagatc cgagctaatg agcatcgcaa tagcttcttt tgagaggccc agtcggtcac
    60001 caatcataag aggaacagcg actgcacctg cgtacatgac aaggacgtgt tgcagaccga
    60061 gtatgatcag ctttcctggt gataatatgc ggtcaacctc atcagttggg cggtcttgcc
    60121 cagaagatga gggaagttgg gaatctatgg cgctcatcag tgtttctcct ttcgcccgcc
    60181 agagtaaaaa gcaaccgttt acgttaatga aactgtctaa acacatgctc gtggagtgcc
    60241 agtttttgcg aggaagggga gttagagaca ggaattgcgg aggcaatcac ataatcgtgc
    60301 ttaaactacg aaaatgacaa gagaacctga caatggaatt atctgtaatt ttctcatatt
    60361 gatatttcat taatcattct ggcatgtcgc actctcgcat ttaatcgttt ttatctggat
    60421 agcgctattt ggggcggcaa tgttgaaata atgaaagcgg catagattga ggggcaataa
    60481 tttgaactca gtcgcaaaat tataattaaa acggtcatgc tacctaatat atgcccgttt
    60541 attgcatcgc gaaatttgag aagacataac tcattaaatg aaagggatgc aaatgaataa
    60601 gtttatcgct gctgaagccg cggaatgtat aggctgtcat gcttgtgaaa ttgcctgtgc
    60661 ggtggcacac aatcaagaaa actggccgct gagtcacagt gactttcgac cacgtatcca
    60721 cgttgtaggg aaaggcctgg ctgcgaatcc ggtggcctgc catcactgca acaatgcccc
    60781 ttgcgttacg gcttgtccgg ttaatgctct gactttccag tccgatagcg tacaactgga
    60841 cgagcaaaaa tgtattggtt gtaaaagatg cgcaatcgct tgcccctttg gcgtcgttga
    60901 gatggtcgat acgattgcgc agaaatgcga cctttgtaac cagcgcagtt ccggcacgca
    60961 agcctgtatt gaagtttgcc caacacaggc gttacgactg atggacgata aagggttaca
    61021 gcagataaag gtggcccgcc agcgcaaaac ggcagcagga aaagcgtcat cagacgctca
    61081 gccatctcgc agtgcagcgt tgctccccgt taactcgcgt aaaggcgcag ataaaatttc
    61141 agcaagtgaa cggaaaaacc actttggcga aatctattgc gggctggatc cacaacaagc
    61201 gacttatgag agtgaccgct gtgtttattg tgccgaaaaa gctaactgca actggcattg
    61261 cccgctgcat aacgctattc cggattacat ccgtctggta caggagggaa agattattga
    61321 agcggcagaa ctttgccacc agaccagttc cttaccagaa atctgcggca gggtatgtcc
    61381 acaggaccgt ctttgtgaag gcgcatgtac tttgaaagat cactctggcg cagtctctat
    61441 cggtaatctg gaacgctaca tcaccgatac cgcgctggcg atgggctggc gtcccgatgt
    61501 cagcaaagtt gttccccgta tcgagaaagt ggcggtgatt ggcgctgggc ctgcagggtt
    61561 agggtgtgct gatattctgg cgcgcgcagg agttcaggtc gatgtctttg atcgccatcc
    61621 agaaattggc ggtatgctga cttttggcat tcctcctttc aaactcgata aaacggtatt
    61681 aagccagcgg cgagagatat tcaccgcaat gggaatcgac tttcatctta actgtgaaat
    61741 tggccgcgat attaccttta gcgatttaac ttctgaatat gatgcagttt tcatcggcgt
    61801 ggggacttac gggatgatgc gagcagatct gccgcatgaa gatgcgcccg gtgtcattca
    61861 ggctctaccg ttcctgactg cccatacccg ccagctcatg ggattgccgg agtctgaaga
    61921 gtatccgctg acggacgtgg aaggtaagcg agtcgtggta ttgggcggtg gcgatacgac
    61981 aatggattgt ttgcggactt ccattcgcct caatgctgcc agcgtgacct gcgcgtatcg
    62041 tcgtgatgaa gtcagtatgc cgggctcgcg caaagaggtg gtcaatgcgc gcgaggaagg
    62101 tgtcgagttt caattcaatg ttcagccgca atatatcgct tgtgacgaag atgggcgttt
    62161 aactgcggtg ggcctgattc gtaccgccat gggtgagccg gggccggatg gtcgccgtcg
    62221 tcctcgtccg gttgcgggtt cagagtttga attgcccgcc gatgttctca ttatggcctt
    62281 tggtttccag gcgcatgcca tgccgtggtt gcagggcagc ggaattaaac tcgataaatg
    62341 gggcctgatt caaaccggtg acgtcgggta tttacctacc cagacgcatc tgaaaaaagt
    62401 ctttgctggt ggtgatgcag ttcatggcgc ggatctggtt gtcactgcaa tggccgcagg
    62461 aaggcaggcg gcgcgcgata tgttaactct gtttgatacg aaggcatcgt gatgaaatcg
    62521 ttaattatcg ttaatccggc tgactgtatt ggctgccgta cttgtgaagt ggcctgtgtg
    62581 gtcgctcacc cttcataaca ggagttgaat gccgatatct ttttaccccg gctgaaggta
    62641 cagcgactgg actgcatcag tgcgccggtg atgtgccatc agtgtgaaaa cgccccttgt
    62701 gttggcgctt gccccgtggg ggcgctgacg atgggcgagc aggtggtgca gaccaattct
    62761 gcccgttgta ttggctgtca gagttgcgtc agtgcgtgcc cgtttggaat gatcaccatt
    62821 cagtcgttgc cgggggatac ccggcaacaa atcgtgaaat gcgatctctg tgaacagcgg
    62881 gaagaggggc ctgcctgcgt tgaatcttgc ccaacacagg cgctgcagtt gcttactgaa
    62941 agagaactca ggcgagtccg ccagcagcgt attgttgcca gcggtgagaa tcctctgtga
    63001 aagaaatctc cgcgctgaaa ggcgcggaga atctattaga ttgcccaacc acccgcgtaa
    63061 aatgcgacca gtgcggcagt aatgatcacc gtaccaatat tcagcttttg ccattcgcgt
    63121 gcaaagacgc ggcctacgac cagtgtcaca aagcccagca taataccggt aacgatatta
    63181 caagtcagaa cgatgaacac ggcacacacc aggccagcca tagcgtcaat aaaatcattg
    63241 aaatccagct tcgagacgtt acttaacatc agcaaaccta cgtacatcag agcgggtgca
    63301 gtggcgtaac cagggatcaa aaatgacagc ggtgataaga acagaatcag caggaataac
    63361 gcccccacta cggttgcggt taaccctgtt ttacccccgg cggcggttcc tgccgctgat
    63421 tcgatataaa ccgctgcggg cgctgcgccc accaggccgg agaatattga acttactgag
    63481 tcactggtca gggctttgcc gccgttgatg atctggttgt ctttatccaa caaattcgcc
    63541 tgaccggcga cggcacggat ggtgccagta gcgtcgaaca ctgcggtcat caccaatgcc
    63601 agtacactcg gaagtacagt tggctggagt gcgcccataa tatcgaggct gaaaatcaga
    63661 gacttaccat cttcgccagt caggcttggc atcgccacca gaccgtggta tttcaccgct
    63721 gggtcaaaga ttaagccgat gatcgaaatt gcaataatca ccaacaagat accgccgggt
    63781 acgcgacact tctccagacc gaagatgacc gccagcccca gcaagctcat catcaccggg
    63841 aaggaggtaa acgcaccgag cgccactggc aagccttcaa tcgggttttt gataaccata
    63901 cccacaccgt tagcagcaat cagcagcaga aacagcccga taccgatacc tgtaccgtga
    63961 gcgataccca tcggcaaatt acgtaagatc caggtacgca caccggttac ggaaatggcg
    64021 gtgaagatga cgcccatcag aaataccgcg cccagtgcga caggaacgct aatttgttgc
    64081 ccgagtacca gactgaatgc ggtaaacgcc gtcaaggaaa tcgcgcaacc aatcgccatt
    64141 ggcaaattag cccataaccc catcagcaac gagccgaagc ccgcgaccag acaggtggca
    64201 acaaacacag ctgcgggagg aaaacctgct ttgcccagca ttcccggaac gacgataacg
    64261 gaataaacca tggccagaaa ggtcgttaag ccagccagta cttcctgacg aacggtactg
    64321 ccacgagcgg taattttaaa ataattatcc agcgcgccct gtggctttgg tgcgtccggt
    64381 gtttgtagga tgtctccaga cataatgatg tcctcacgag gatgtaaaag ttttattatt
    64441 agttgcgttc gtacaccaga cgaccatcaa cgtaggtgcg gtagatcgaa cggtcatcgc
    64501 ccaacgtcat catcacgaac aatttgtcga ctaaagaaac agagttgtca tagcgcagct
    64561 gctgtagcgg agtggcggtg ggttccatca ccacgaaatc agcctctttg ccaggtaaaa
    64621 agttgccaat caaatcgtca aggcccagag atttcgctcc gccgagcgtg gccaggtaaa
    64681 acgcttcata tgccgagagg cgatagcctt gtaattgcaa cactttgtag gcttcgttca
    64741 gcgtttgcag catgttgaaa gtggttccgg caccgatatc cgttcccatg cccactttaa
    64801 ctttcttctg ccatgctttt ttcaagttga ataacccgct gccgaggtaa aggttggagg
    64861 tcggacagaa agcaatgctg gatttggttt cgctgagacg atcccactct ttttctttga
    64921 gatggacgca gtgagcaaag acacagtttt taccggtcag gccgtactga tggtaaacat
    64981 ccagataacc atcatggtca ggataaagcg atttcaccca ggcaatttca tctttgtttt
    65041 cacagagatg ggtatgtacc cacgtatccg gatattcctc tttcaggcgt tgcgccatcg
    65101 ccatctgttc aggagaagaa gtaggggcga agcgtggcgt aatcgcatat agcagacgac
    65161 catttttgtg ccagcgttca atcagttctt tgctttggtg atagctgctt tcggcagtgt
    65221 ctagcagata atccggtgcg ttgcggtcca tcatcacctt accggcaatc atacgcatat
    65281 tgatatgact ggcggcttca aacagcgcat caacggattg cggatgaaca gtgccaaaca
    65341 ccagcgccgt ggtggttccg ttacgtaaaa gctgcttgat gaagaacgcc gacatttcgc
    65401 gggcgtactc taaatcctca taacgacgtt cagtagggaa ggtgtgttta ttcaaccact
    65461 ccagcaattg ttccccatag gcccccacca tttcactttg cggataatgg atatgtgtat
    65521 cgacaaagcc cggtactatc agtttgccgc gatagtcgcg cacgcgaata gtgtcaggaa
    65581 tttgatgctt tccgtcttcc cattcgccaa accattccac ttttccctgt ttaatgagta
    65641 ataaaccatc ctcaataaac cgcagcgcag aggcaatctc ttccgggtta tcgaccgtac
    65701 gggcgacatc aataaaactg cctcgtaccg cttttaacgt gtgttctcct gacatcattg
    65761 actcctttac tgttaatcca tctcttccgc tgaggtaata ccaggcagaa cgttttcctg
    65821 tcggtagcca ccagggagga taatatttaa taaaatcgca gttaacccgc cagcacaaat
    65881 agggttttca actaatacat aaatagaggc tggcaatatt ttaaaaattt cgggatcgta
    65941 ggagacgcca agtcctaaac ctaaagaagt ggcgacaata agtgtttctc ggcgctttaa
    66001 accgttggtg atgatgatgc gaatcccagc gatggcaatc atggaaaaca tcaacgtcat
    66061 tgcgcctccc agaactgccg agggaatggt cgtgaagaag ccgccaatca tcggaaacaa
    66121 accgaggata accagcatta ccgcgatggt tcgcccgaca taacgtgaag cgacgccagt
    66181 catctgaata accccattat tttgcgcaaa cgtggttaag ggtaatgacc cgacagcgga
    66241 ggcgataaca gaaaccagac cgtctgccag cacgccgcct ttcagtcggg actgatactc
    66301 ttctccctga atgggtcggc gggaaaccat tgccgtggcg gtgatatcgc cgacagcttc
    66361 cagcacgcta agcagataaa tcgtgccaac caccaggaac tgatggaagc taaaactaaa
    66421 gccgtatttg aacggatgtg ggatggtgat taacggcaaa ttgcgcatac tgctgaaatc
    66481 caccatgccc aggcataacg acgcgatata accgacgcat agcccaatgg cgatccctcc
    66541 catgcgtagc aacggactgc gacagcagtt aaagccgatc accacaatca aaaccaataa
    66601 accaacgccg agatgttcgt aattgccgaa ggtaccgctg cttttggcag caaatcctcc
    66661 accaaaatcg ataatgccga ctttaatcag gcttaagccg atcatcagta ccacaatacc
    66721 gctgacagta ggcgtaataa cccgacgtaa atagggcaag ataaatgaag atccgacaac
    66781 cagaaatgcg ccaacgaagg agacgccgag gagcgacgac atgattaact cttcgtgaaa
    66841 accgtcgctt ttcatgctgc tgcccagcgc aatcatgacc gtaacaaatg aaaaattgac
    66901 tgactgaatt gagagtaggc cagaaccgac gatgccatag cggtttactt gtaaccaggt
    66961 accaatacca gaggcgatca tcgccattga aacaagatag gcagttgttt cagcggaaag
    67021 ctgtaaggcc gcccccacga ttaacgcggg ggttaccatc ggaacgaaaa ttgccaacag
    67081 atgggtaatg gcaccaacga gagcctgatg aaagggaggg cgatcctcca gttcaaatat
    67141 aaggtcagaa cctgcatggt ttatatcaga catcccttca ctccttttaa agtaaatttg
    67201 atcatctgaa ttagtaaaat aaattcccac gaatttgggg cgcaggacgc cttgagaacg
    67261 gcttatctca ttttcgcagt cactatttag tcatcgtcca aacagagaaa caggccgccg
    67321 aagcggccat ttattattta tatcttttcc agcgcggtga gtattttctc cggtgtgaaa
    67381 tgccattcgc gtaaccagat gccgcatgca tcgtgaattg cggtagcaat cgccggagct
    67441 gcgccattta caccgatttc cgagatcgat ttcgccccga acgggccgac tttatcgtcg
    67501 ctcggcacca gcactgcgcg gaaatcacgc ggaatgtcac caattttcgg tgcgccgtag
    67561 ctgcgtaaat cacgcgttaa cgggtgacct tcggcgtcat aaatgatctc ttcgctcata
    67621 ctgtggccga tagctcgcag ggtagcaccg tagatttgtc ccaacgctaa ctctggattg
    67681 accggtgtac cgcagtccag caaggcgtag aatttatcca ggcggatttc acccgtacgc
    67741 gtgttgacgg caacttcagc gaagtttgcg ccatacggga aggcgaaatc aggcgtgata
    67801 taactgccag ttcccaccag tgaaccaaag ccggtgccgg tttcgccttt atgggcaata
    67861 tccccgaaac taacttcgcc tttcttgccg cgcacgacgc ccggcgttgc tagttgaaca
    67921 tctgccactg gctcacccaa catttgcgcg ccgtggaaca gaattttctc ccgcagattt
    67981 tccgctgcca aacgcgccgc gttacccgag aagcaagtac cggacgaggc atatgcgcct
    68041 ttatcaaaca acgcatgatc ggtatcaccg gagataacat gcacgtcctg cggtgggcag
    68101 tgcagcactt ctgctgccag tttcgtcacc acggtatcca gaccagtacc aatatccgca
    68161 ccgccagaat gaacgataaa ggtaccgtct gattccagtt tgatcatgca gttagcctga
    68221 tcgatatccg ggatccctga tttctgcatg ataatggcga caccacgacc gatgtgccag
    68281 tcaccatttt gtggttttgg tgaagaccat tggatcatct cgcgcccctg acgcaggatc
    68341 tcttccagtg cgcagctggc ggcggaagga acggaggtcg gcgctttacc ttcaccgatt
    68401 gcaccgagaa ttttcagctc ttgcccttcg tgtacccggt tacgttcgat aatttccagt
    68461 tggtcgatct gtaactgttc agccagttcc gctaatgcca tggtgatagc gaagttacct
    68521 ttcggtgcgc cataaccctg ataagcacca tttgggcaaa tgttgctgta gtaggtggtg
    68581 acctggaaat cgacgttatc gcacggatat aacggcagcg acagcgccgg tccgttacac
    68641 ggtacggtga gtgagtggtt gccgtaaggg ccagtgttgg cgcggaaatc catcttcact
    68701 gccgtcaggc gaccatcttt tttcgctccc agtttgacgg tgactttcgc gacgtgacga
    68761 gaggtgttag caataaactc ttcttcacgg gtgtagcgga acagtaccgg acgcccggtc
    68821 acgcaggttg cccaggcgca cacttcttcc agcaggatgt cctgtttgga accaaaaccg
    68881 ccgccaactc gctctttaat gacatgaact ttatgctgtt tcatgtccac gaggcgcgcg
    68941 acctggcggc gtaagtgcca tggtacctgg gtggaggcgt ggataaccag acgatcgccg
    69001 tccatccggg taaagcagat atgtgtttca gtcgggcact gctgcgcctg cgttgagtta
    69061 taggttcgct caatgatcac atcggcatcg gcaaagcctt tgtccatatc gccaatatga
    69121 ccatgaatac tagcggcgat atttttgcga gggcgagaac cgatcgggaa gttgatgatc
    69181 atatgctcgc cgcgctgggc tgcatggctg ttatcgtctt ccagagtatc tggcgcacca
    69241 gcaacataca ccaccggttc atcgtgcacg acaggcgcat cttccgccat tgcttcgtcg
    69301 atcgacatta ccggcttaag cacttcatat tcaacctcga tgagtttcaa tgcttcgagc
    69361 gcaatttctt cactttcagc gaccaccgca gcaacgcgat cgccgacgtg acgcattttt
    69421 ttgccgaaca tacggcggtc aagcggtgac ggttccggtg cgctctgacc ccccggggta
    69481 tagtagatat cagggcaatt caggtgagta ataacgtgaa cgacgcccgg taaggcttca
    69541 gctttgctga catccagatg agtaatcagt gcgtgagcgt gtgggctacg taacatttta
    69601 atgacgcagg cgtcagccgt tacgcggtct tcaacatagc agggtttcgc ctgcaccatt
    69661 ttcgcggcat cagttttagg ataatgcttg ccaatgactt caaggtcgtc acggaaagtc
    69721 ggagcgatat caatggtggc ctgcggatta tttttacgtg caaccgccag ttcaatgacc
    69781 tgatagtatt gctgccagcc agcatcgcgg ctgaacaaac cagaaagcgc gtcgtcgatc
    69841 tcttcgcggg taggtgcggc gatgcgatcg agaagatcgg tgataatcag agctgcagct
    69901 ggatcgttat aaccagactg caccacgcca acatcaacca ttgcctgttg aaccagactt
    69961 aactcgttcc atttgcccag agattctgcg gtacgaatat ctgccttctc taactgtgcg
    70021 gcaataagca aggacgcgtt aacgatatta ccgttaaaga tgattgcgtc agaaccggca
    70081 aacccgaaac catcatcact gttgcgtaca gagtgcattc ccatgttaaa caacagcttt
    70141 tgcacgtttt cgcctggatt aacggttagc tcctgaggcg cgccatttaa agtaaagtgg
    70201 atgatcatac ggcttcctcc ccagcctgtt ggcagtcggc atacaggtcg gctaccagaa
    70261 ctcccgtgat atagcgttta taggccacgc tgccccgcaa atcttcctgc gggaaaatgg
    70321 cgttggcgac agcctgttca agtgcattgc cttccagatt ttgtttttcg acatcatgca
    70381 gacgcagtgc tttactggcg acgccatcca gcgcaattcg cataccgtcg tggtctgtca
    70441 ttgcaacggc tgccgtcacg acggttaaac ctgcctgaga acggctaatt ttgcgggtcg
    70501 cacaggtgcg atacggatct ttaatgataa tttcggttag caggcgatcg catgggcagg
    70561 ccaggtagtc ctcgattgac agcgtttcgc cgttgccaaa aaccagttca gcatccagtg
    70621 ccagcaggac gggaagcagc actgactctt cctgccgggc ggcgatttca ccaccaatgg
    70681 tcgactgatt acgaacatgg cgtgagtaaa caaaaccgag ggcttcacgc agtgctgcag
    70741 gaataaatcg cgcatcacgc agtggctgca agcgagacat tgcgccaatc cgcagtgcac
    70801 cgttatccca gtcaacccag tccagttcca gatcctgtaa ggaaatggca atctttttat
    70861 cggtacgggt tggtgtagca ttgagtttgc tgcccccggc gaaccagacg gcttcatcct
    70921 ggtagcggcg cttcagttcc agcgcctgtt cgacagagtc gggcctgaaa aattgttcaa
    70981 tcatcttctt tccttattta accctcttcc tgcgggaaga gggcatctgt catttatgcc
    71041 agcgcatcca tccgacgcca catactggca gcggcttttc tggcttgtgc ataaatggaa
    71101 tcgcaatcga agttaaactg acggtcttca tagaccatca caccattgac catcacgctg
    71161 tgaacgctgc ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccagcagc
    71221 ggcgtcggcg agttgtaatc gcaaatggtt aaatcagctt tgtaaccggc ttccagaagc
    71281 ccaaatttcg cgccaaagtt gcggctcatc agttcgttac cgttagtcag ggctttggca
    71341 aaactgtcag gccacagcgg accacccgca tcgcgatgtt taaagaaggc aaatttcatc
    71401 tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta
    71461 aggtgatggt tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaac
    71521 gcatcgcgct gattgagtag ggcgatgtca tctttcgaca agtacagccc atgagcgacc
    71581 agcgttttgc tatcgatgag atcgaattgc gccagtcgtg ccagcaggtc tttgccgtac
    71641 cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag cgtgaatatg caaaccacgg
    71701 cctgtggcgt tcacggcttc acgtagcatc tccagaccgg catctggcac ggtaaacggc
    71761 gcgtgagcac cgatatgtgc ttccaccaga tacggctcgg ttgctgcttt cttcgcctca
    71821 tcaatctgac gggcgaagcg gatgttttct tctacacctt cctgcaactc tttgatgccg
    71881 ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg
    71941 tcgcgcaatg tggagagcga cccgccgata tacgccggag aggcatggtg atcgataacc
    72001 gatgtacatc cgctcttaat cgcttccagg gaacaaatca gcccgctgta atagagcgac
    72061 tcttcatcaa gggcgcgatc gagccgccac cagagatttt tcagcgttga gatgaaatct
    72121 gggctggggg cgatgtttgc cataattccg cgggaaagcc ccgagtaaaa atggttgtgc
    72181 gagcagacaa ttcccggcat cacaatccga ccatgcatct ctttgtagct ggcatcgggg
    72241 tagcgttgcg tcagggcatc gccgatagcg acaatcacat cattttcgat agcgatatca
    72301 acgccttcct gcactttcgc cgggtgtaac tgcactgcgg tgacattctt cagaatcaac
    72361 atgattacac ctccacgcgg cccagcagat aatgatgatg ctggtggaca tggctgatga
    72421 tgcggcacat atcgttcagc tccggtggta cgttgttgaa ctgaccttcg ctgtcgatgt
    72481 ttaacaccca gctttggtta ttcagtcgta ctcgtacccg gcaatcttcc acaaggaagc
    72541 ctgggttgct gctgttatca aagtcttgcg ccaggctgaa aacggtgatt ttgtctttgt
    72601 atggtttacc gttccacgga cagaactgag cgcagttgcc gcattcgtta cagtaagcgt
    72661 cgaggtgcag cgtctggaat ctattctgga agcctgggac cgcaatggag acgttggcgc
    72721 ggttcgggca gacatccaca cacttgctgc aaacgtagtt acattcaagg cagcgagcag
    72781 cttcctgcgc gacaaacgca tcacgatcgt cactgttcac cagcgtgata gagatatcgc
    72841 ctttacgttg atagatttcc gccggattga cgttgttcca gtatttatcg ttctggtggg
    72901 aacggatatt ttcccgacta aggatggcat cggtcgcccg acgcgcggtt ccgacagcag
    72961 cgacaatgga ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgag
    73021 tttcgccatt atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccatcgcat
    73081 tcagggcttc agtatcctgc tgttcaccaa tggcggtgat caggctgtct acatgcagtg
    73141 ttactgtttc gttggtttca accggacgac gacgaccttt ctcatcgggt tcgccaagcg
    73201 acataacgcg caaggttaag gtgccatcag catcgaaacg ttccggatta ttcaggaaac
    73261 ggaactccac gccgtcgtgc aacgcttctt catactcttc gcgccatgcg ggcatctctt
    73321 gcagtgaacg acggtaaacg accgttgctt tttctacgcc tggaacgcgt aacgccgcac
    73381 gagcgcagtc cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga
    73441 gcgctgtacc cttgttgtat tcacgcagga aggggagtga tttcaagaca ttttggttgt
    73501 cgcccgccag tttcacaccg ctatttttat ctgtgccggt ggcaatcaga acatagtgga
    73561 agtcctgatt ttttaactgc tcaacggtta aatcgggtga gcagccatac tcaaatttca
    73621 cgccgtgagc ggcaacaaaa tcgatatcgt gctgaattaa ctcagccgga attcggaact
    73681 gaggaatgat atttttcacc acgccgcccg cattggcttc gcgttcaaac agcgtaaccg
    73741 gatggcccgc tctggcaagg aagtaacctg ctgccagacc cgccggacct gcaccaatca
    73801 cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat
    73861 cccaaccttt ttccagcgcg acttttttca gttcgcggat attcagcgca ctgtcgtaat
    73921 ccaggcgggt acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca
    73981 gagcgttgcg ttggtagatg agttccagcg cgtcggcata gcggtgttcg ccaagcagac
    74041 ggatgtattc cggaatatct tgcttgatag cgcaggcagt aacacagggg gcaacgtagc
    74101 agtcggtcag cggcaggtct tctgccactt caatacgctc ttctggcttc cagtgtttct
    74161 gggtgtattc catggttaac gcatctgctg ccagtctgtt cagtcgttcg acgtcaacat
    74221 ggtcaagtcc ccaggcgtcg gagccttcca gctcgcgcat gcaggcactt aagcgcaaat
    74281 agccgccagg tttcagcagg tcggttgcca tagtaatagg gcgaatacct gtatcaaaaa
    74341 tatcgcggat agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa
    74401 aggcgcgaga gagaactgct gcaacattga tggagagcgg gaacagcgca cggcctgaca
    74461 tatacatctc ttcaccaggc agtgcgcctt tattgttgat ggtgccgaga gtgttagtca
    74521 gttttacgcc aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt
    74581 ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc ttcttttaag cctatgtaac
    74641 cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta ccccagtaag gtcgggttaa
    74701 gtttcacaaa ggtgttgagc cctttttctt ccagcatgta gcggcaaatg gcttcgattt
    74761 catgcggagg acagccgtgc atggtggaga gggtgacgcc atgcaccata ctggtgggga
    74821 tgcgagcggg taaggcttgc aagctttcgc gtttttcctg caatccgtgg cgggctaaaa
    74881 atgcgtcatc ctggagtaat ttattcagcg tatcgcgata ttgcgcgaat ttcggatggt
    74941 cagatgcgtc catcatattg tcgatgaact gttgcattgg cggttgctta ataccttcga
    75001 ggttgtaacc gacgctcata ttaaagatga acgatttacc ggaatcagaa ggttggaaca
    75061 tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca
    75121 gggtaaattc ggtagaccat tcggtgttaa agcactcgtc ttcggcatca atacagggct
    75181 tttccagctc caggcggtca agaatttgga cggtttttag ttcgatgaaa cgtccgccag
    75241 tcagccagga agtgacaata ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga
    75301 cgggagtggc acaggtttca ccgaagacgc taacagtttt accttttaca ggtgagtaaa
    75361 actgttgctc gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg
    75421 tcaaaagttc ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt
    75481 atacagatat cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa
    75541 caatcgcacc actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa
    75601 acttaagggc tttttgtgaa tgaactcaca ggtgctaaat gagaattaaa atcactcctg
    75661 aaaattatta ttgccatccc atatatcact ttttctcttt tctcaaaatc agtgattcct
    75721 tatcattatg ataaagagtg ctggagggat taaatatttg aacgcaatcg tttaactacg
    75781 ttttttaaat tttagtatac cttttttgcg ctgtaataaa gtcgtccgga gtatcaatat
    75841 ctaaaattat ttctgcattt tctacttcga cggaataatg agctcccata aggagtgcct
    75901 gacgcatatt ggtgatattg gggcgcttga ttgcctgcat caggcatggt tttgacacta
    75961 aaatcggatg gccggggatg ccattatgga gtggcagtat tgcgccatca tttcgtaacg
    76021 accagatttt tctgaaaata tcgatggtga gggttggcat gtcaccgtgg gtgagaaaac
    76081 aatgttctgt ttgcactgcg ggtactgcgg cctttactga tgttagtaaa ccctgcgcat
    76141 aatctgggtt gtggataata gtgatattgc tctggttcgc ataacgttcg tgcagttcat
    76201 taccacgata gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac
    76261 ttgtatcaag aattgtcccc tgttgccagg gtaacatcat tttccattgc cccattcttg
    76321 atgataatcc ggcagcggta attatacagt cgatggctga catagtggtt ccattattta
    76381 taggggctaa taacgcctga taattgcgtt acttcctgat ggattaagtt ggggataatt
    76441 tatcccagag aggtcataaa gactcgtgaa aagtatagtt gacccatcgg cattattcat
    76501 tgatttaggt gcgcagaaac gtcccactgt gatttctgtt gttggtgctg gggggaaaac
    76561 cagcttgctt ttttggctgg cagaactgct ccaggctagc ggcaggcgtg tgttaatcac
    76621 gacgacaaca catatgttta tgccaacgtc tcactggccc gtggttttct gtcgtgatcc
    76681 cgccatgctt cctcatgcgt ctcttacatc ccccatttca ttttgttttc acagctggaa
    76741 agcgaacctg ggaaaagtgc agggatttac gccagaagcg attgatgcac tggtgcaacg
    76801 accagagtgt gacgtaattc tcattgaagc agatggctcg cgtgcaatgc cgttaaaagc
    76861 gcctgatgag cacgaacctt gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg
    76921 aggacatatt ttgggcgcga aagtgagcac ggaaaatgtc catcgctggt cgcagtttgc
    76981 tgatattacc gggttaacac ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt
    77041 tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat
    77101 taaccgtttt tctcaatgtg agaatgcgat tgcgcaaagc gagctccttc aaccgctgca
    77161 acaacacgac gtagaggcaa tctggctggg cgatatacaa gaacatcctg caatcgcgcg
    77221 cagatttgtg aattagcggc ccgggtattc cgggttactg aaaggatatc cattgaggtt
    77281 ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc aaaattgtcc gtttgctatg
    77341 gcgcaaatta ttgatagccg aggctcgact ccccgccatt ctgcacaaat gttagtgcgc
    77401 gccgatggct ctatcgtcgg tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa
    77461 gagtcgcttc aggcattgca ggaacgtaag ccgcgattat tccatggacg tatggctcgt
    77521 aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcga tgtcagtgtt tatcagcgtc
    77581 catggtatgc gtccacgtct ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc
    77641 cagagtgcgg cgctattagg atttgatatt gccgttgccg atatttatcg cgaaagcctc
    77701 aatcctgaac tattccctcc atcaaccacg cttctccatg ctgagtcgtt tggtgcggca
    77761 gtggaagcac tggatattcg ccctgataat tttgtcctga ttgccacgaa taatcaggat
    77821 cgtgaagccc tcgacaaact cattgaacag cccattgcat ggttggggtt gctggcaagt
    77881 cgtcgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat
    77941 attgcccgtt tacatgcgcc cgttggttac aacataggtg cggaaacgcc gcaggagatc
    78001 gccatcagcg tgctggcaga aatattacag gtgaaaaata acgcgccggg tgggctgatg
    78061 atgaaacctt ctcatccttc cggacaccag ctggtggtga ttcgcggtgc gggagatatc
    78121 gccagtggtg tggcgctacg tctgtatcat gcgggtttta aagtgatcat gctggaagtg
    78181 gaaaaaccga cagtgattcg ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa
    78241 atgacggtcg aaggcgtcac tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt
    78301 accgaacgcg gattcatccc tgtgatggta gatcccgcct gttcattgct tgatgaactg
    78361 aaaccgcttt gcgtggtgga cgctattctg gcgaaacaga atttgggaac acgggcagat
    78421 atggcaccag taacaatcgc gcttgggccg ggctttgctg cagggaagga ttgtcatgcg
    78481 gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga tttactgtgg ttgtgcgcag
    78541 gagaataccg gtgttcctgg caatattatg gggcatacca cccgacgggt gatccgtgct
    78601 cctgctgcag gcattatgcg atccaacgtg aaattaggcg atctggtgaa agagggcgat
    78661 gtgattgcct ggattggtga gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc
    78721 ttgttgaacg acggcctggc agtggttggt ggttttaaaa ttggtgatat cgatcctcgt
    78781 ggtgaaaccg ctgatttcac cagcgtttct gataaagccc gggcgattgg cggcggcgta
    78841 cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa
    78901 gtggcttaaa tgaaaatggc cggatggtat tacccacccg gccttaaaat tactgacaaa
    78961 tcaccgttcc ggttttgcct tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg
    79021 cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc ttccacttta ggcagcatag
    79081 aacctttcgc aaaatgacct tcttcgataa agcgttcagc atcacttaat gacaggcgat
    79141 cgagccactg ttcattctct ttaccgaagt taatggccac tttttctacc gcagtcagaa
    79201 tgatcagcat atcggcatca atcatttctg ctaaacgggc gctggcccag tctttatcga
    79261 taaccgcgct ggcaccacgc agatggttac cttcacgaat aacaggaata ccaccaccgc
    79321 caacggtgat caccacctgg cctgcttcta ccagcgcttt taccgtctct ttttcaatga
    79381 tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc gcgaccagca tcttctttca
    79441 gagtgtaacc ctgttttgtc agttgctctg cttcttgctc ggtaaagaac gaaccgatcg
    79501 gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg
    79561 ttactacagg tttattaatg ccccgagaaa gcagttcttc tcttaaggcg ttttgtagat
    79621 cgtaaccaat ataaccctgg cttaaggcaa cacagacaga catcggcagc attggcgaat
    79681 gcgcttcagt ttttgcggca gcttcaaagg cctggttaat catgcccacc tgcggcccgt
    79741 taccatgagt gacgataact tcatgtccct gggcaattaa atcaacaatt gcctgagaag
    79801 taatttttac cgctttcatt tgtccggcca ggtcatcgcc cagcgcattc ccgcccaggg
    79861 cgagaacaat tttcttactc atttttcctc actcatcatt cttgattgtg tatagatccc
    79921 tggcagtggt tagagcacgg gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc
    79981 ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca ccacgggata aggttctgac
    80041 aatcgcgccc tgacaggtaa aaccctccca tggcgagtag tcggcgttgt cgtggagatg
    80101 gcgatgctga atttgttggc tctgacgtgg gtcgataatc accacgtcgc catcggaacc
    80161 gggcgctaat aatccttttt gcggccacag gccaaacagc ctggcgggca ttgcgctggt
    80221 taattcaaca aagcgttccg gtgttatacg tcccgtcatc acgccactgg aaaacagtaa
    80281 ctgcatgcgg ttttccacac cgggtaagcc atttgggcag cgactgaaat cgcctttaga
    80341 aatttgcagg cgttgagcca tcgagaaggt gcagtgatcg gtcgccacca cgtcaatcgc
    80401 accatcgctg atgccacacc acagtttgtc ctgctcgcgt acgttacgca gcggtgggct
    80461 aagaatgaac ttcatgccgt cttctgtatc gtaactgcgt tcgtccaaca ggagatattg
    80521 tgggcaggtt tcaacccaga ctggctggtg attcgcacgg gcaagacgca gataatccag
    80581 acctaagccg ttagacaggt gcacgatata gagcggggcg ttaccggcaa tttgtgccag
    80641 gttaatcatg cgggcgatgg cttccgcttc gcattccaga gggcgactca aggcatgata
    80701 gcgcggcgcg gttaaccctg cggcgataaa ttccgcccgc ttgctggcga tagccgcatc
    80761 attttccggg tgcacggtgg tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa
    80821 aacctcgtca tcgttgagtt tgtattgata ggttaaatag agtttaaaac tgctcagccc
    80881 ttcctcgacc atcatcggga tttcgtcgag gatggcgtga ttaatgtgct ggatcacacc
    80941 gtgaaagctg taatcgatga ccgctttatg ggcggcataa ccacgataaa cctccagttg
    81001 atggcgtaac cgacagccgt ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc
    81061 gccacacgca gctgcgcggg taccggtaaa aaaatcatca caactgcgcg cgatgccgac
    81121 atcaatattg aaatgcgtat ggacatccac gccgccaggg aaaacgtaac agccagtggc
    81181 atcaatttct tcatacggga gctgcggcga aatattgttg cccaactggc gaacaatccc
    81241 gctttcaatc agcaaatcct gtttggcttg tccatctgcg ttaacgacag tgccgttttt
    81301 gatcaatacg cgcatagcaa actccaaaaa ccctccggcg aaccggaggg gaataagtga
    81361 agagtaatta ttcggttgcc agccagctta acgggattgc agcgtacatc gcggcacagg
    81421 tcaccaggtg agatttccag gttttttcgt taggtgcatg cgcttcaggt tctttacccg
    81481 ggccaaagcc gatcaccgga atgccgtgac ggcccatgat agatacgccg ttagttgaga
    81541 aggtccactt atcaacaacc ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg
    81601 ctttaacggt gaagtgatct tcttccactt tccaggtcgg gaagtagcat tcggttgggt
    81661 aaaccaggcc agtccaggac ggacggtcgt agttgtacat agaaacaacc gcgttagctt
    81721 tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg
    81781 tcagacggcg gtcaatagag acggcgcagc tatctgctac agcgcaacgg cttggggatg
    81841 tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag gaattcgtca taacccagac
    81901 gttgggagag ttcttgtaat tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac
    81961 cgcgttctgg cgcagaaccg tggcagctaa caccctgaac atcaatacga atttccatac
    82021 gaccgcgctg accacggtat acctggcagt cggttggttc ggtactgacc acaaattccg
    82081 ggcgaatgcc ggattgttca ataatgtact gccagcacag accgtcgcag tcttcttcct
    82141 gcacagtacc ggtaaccagc agggtatatt catcttccag accgaggtct ttaatgattt
    82201 taccggcata aaccatagat gccatgccgc cttcctggtc ggaagtaccg cgaccaccga
    82261 tcagctcatc agtttccatg ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa
    82321 tgccgacggt atcgatatga gcgtccattg ccaccagacg cgggccgtgg ccgatataac
    82381 cgagaacgtt acccatcggg tcgatttcaa ctttatcgaa gccgactttt tccatctctt
    82441 ctttaatacg atgtactacc cgtttctcgt cgcagctttc actgggaata gcaaccatgt
    82501 cgcgcaggaa gcgagtcata tccgcctggt aatcttttgc tttttcaaga atcagtttga
    82561 atggaatatt cttagccatt atttttctcc agatttttta ctccccgttt tgcagggagc
    82621 agttccaata gggtgattaa ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt
    82681 agtgcttcac gtcggtgtcg ccttcagtgc tgataaccag cactacggca tctttgttca
    82741 gcgccagttt ttccatcagg ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc
    82801 ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg gtcgttgccg tacggattac
    82861 ccagcacgcg catacctaat gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc
    82921 agttacgtag gatttcccag cccagcgggt taggttcgcc acaggccagg cctgccatga
    82981 tggtggccat atcaccgcca acgttgacga tgtcgccttt gacgccggag cgataaatac
    83041 agtcagcttt gtcaggttca acaataatgc tgtgcagatt ttgcgggcta tagacgtcga
    83101 ccagataacc cagcacacca ccggccattg ctccgacacc ggcttgcagc agaacgtgcg
    83161 tcggggttac gcccatttca cgcatttgct cgacggcttc atctgccagg gttgcgtagc
    83221 cttgcatgat ccaggttggg attttggtgt aaccttccca tgccgtgtcc tgtaccactt
    83281 cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat
    83341 ctgtgacgat gcactcggca ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac
    83401 ctttcggcat gtaaatcacc gcattctgtc cgagttgctg cgctgcccac gccacaccgc
    83461 gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt ttcgccgatg gcatttttca
    83521 ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt ttcacacaat aattgagcga
    83581 tagcgtacgc accgccgagc attttgaagg cgttcagacc gaatcgtttt gattcgtctt
    83641 tgacaagaat tttcttcacc ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg
    83701 ttgggcgata accagctatt ttctggtgga actggcgcgc cagtttggcc tggctttgcg
    83761 aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc ggcgatatca atcttcaatg
    83821 agaaaacgga catatcctat ccttttggcc tctggaaata gagggccgac cagctgatcg
    83881 gcccgagggg ttatttcacg cgttcttgcg cttctttcag cagttgctcg agcagtgcac
    83941 ctggtttggc gtatttacgg gacaggatca tcgcggcgat gatataaggc ttccagctgg
    84001 cttctttgta ggtagcgata cggtattttt cgaatacgcc ttcagtcact tccccttctt
    84061 tgcaggatac gccgctgata tcagctggca ggcagtgcat gtacagggct tcgccatcac
    84121 gggtcagttc catcatctct tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca
    84181 gacactgttt ttccagtgct tttaagcctt cgtgatcgtt agcgcgcagc aattcggtac
    84241 gctgttccat cactttgtaa ggtgcccatg acttcggata aacgatgtct gcatctttga
    84301 acgcttcttc catgctggtg acctgacgga agctaccgcc agatgcttta gcattgtttt
    84361 tcgcgacttc tactacatcc gggatcaggt cgtagccttc cggatgggcc agggtgacat
    84421 ccataccgaa gcgagtcatc agaccgatga tgccttgtgg tacagagagc ggtttgccat
    84481 agcttggaga gtaggcccag gtcatggcga tttttttacc tttcaggttt tccagtgaac
    84541 caaagtgttc acgtaaccat gccaggtcag ccattgactg agtcgggtgg tcaatatcgc
    84601 attgcaggtt cactaaagcc ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa
    84661 gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa
    84721 taccaatagc gtcggcgcag aaggagatca tattggcggt ttcacgcacg gtttcgccgt
    84781 gagcgatttg tgatttgcct tcatcgagat cttgctgtgc aaggccgagc aggttaagcg
    84841 cggaagcata agagaagcgg gtacgagtgg agttgtcgcg gaatacggaa atacctaatc
    84901 cactattaaa gactttggtt gagatgtttt cagcacgcag tgcttttaat gctgcggcaa
    84961 cgtccagtac ttgtttcagt tcatctggag tctgttccca cgttaacaaa aaatctttct
    85021 cgtgaaggtg agaggtcagc gaattgatat ccttaatcag ctcattaaca gttttcatca
    85081 taaagtcccg gtagtggaat gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt
    85141 gattggtgtt ttcacagcta tacctgggac taacaataag tgtgccagaa ttatccacgg
    85201 ggctgcaaaa gactaatttg acggataatg tgtggggttg atcacaaatt gatgccagct
    85261 tccggttgtt tgtaaaatac gaaaacgttt ggcttttttt atcgcgttat ttatttgctg
    85321 ctaagtatca gaaataagat aaagggacag gctatccctc gtcagtttga taaactcacc
    85381 gccataattc tcatattgat atagaaatgt gatatctctt ccataatttc attagaaagt
    85441 gataaaaatc ttatgttttt atcaaagcca atttcgcctg agttaatatg atcatgatct
    85501 gtgaaccatc aacgtcttca atgatcgact tttaagtaag tctggaaggt aaagctatgg
    85561 agcttgctac tacgcagtca gtattgatgc aaattcaacc gacaattcag cgttttgcca
    85621 gaatgcttgc cagcgttttg cagcttgagg ttgagatcgt tgatgaaaac ttgtatcgcg
    85681 tcgccggaac gggcgcgtat gggaagtttc ttggtcgcca gttgagcggc aactcacgcc
    85741 tgctctgcca cgtcctggaa acgaaaactg aaaaagttgt gacacagtct cgcttcgatc
    85801 ccctttgcga aggttgcgat agtaaagaaa attgccgcga aaaagcattt ctgggtacgc
    85861 ctgtcatttt acaggatcgt tgtgttgggg tgataagttt gattgccgtt acccacgagc
    85921 aacaagagca tatcagtgat aatttacgcg aattttctga ttatgttcgc catatatcca
    85981 ccatttttgt ttcgaaactt ctggaggatc aggggccagg agataacatc agtaaaatat
    86041 tcgcgaccat gatcgataat atggatcagg gcgtattagt tgttgatgat gaaagtcggg
    86101 ttcagtttgt taatcagact gccttaaaaa cacttggtgt tgtgcaaaat aatattattg
    86161 ggaaacctat ccgtttcaga ccattaacat ttgagagtaa ttttacccat ggacatatgc
    86221 agcatattgt ttcgtgggac gataaaagtg aattaatcat tggtcaattg cataacattc
    86281 agggccgaca attattttta atggcgtttc accaatcgca taccagtttt tctgtagcaa
    86341 atgcacctga tgaaccgcat attgaacaat tggttggcga gtgccgtgtt atgcggcaat
    86401 taaaacgact cattagccgt attgcaccca gcccatccag cgttatggtg gttggtgaaa
    86461 gcggcacggg taaagaagtt gtcgcccgtg caatccataa gttgagcgga agacggaata
    86521 aaccctttat tgctatcaac tgtgccgcga ttccggagca gcttctggag agcgaactgt
    86581 tcggttatgt taaaggcgca tttactggcg cttctgccaa cggtaaaaca gggttgattc
    86641 aggcggcgaa tacgggcacg ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc
    86701 aggctaaatt actgcgcgct attgaggcac gtgaaattct gccgattggt gccagtagcc
    86761 caatacaagt cgacattcgc atcatttctg caactaacca gaatttggcc cagttcattg
    86821 ccgaaggtaa attccgcgaa gatctcttct accgacttaa tgttatcccg ataactctgc
    86881 caccgctgcg tgaacgtcag gaagatattg aactactggt gcattacttt ttacatctgc
    86941 atacccgtcg tctgggatcg gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc
    87001 gtaagcatcg ttggcccgga aacctgcgcg agttaagcaa tttgatggaa tatctggtta
    87061 acgttgttcc ttcaggtgaa gttatcgaca gcacgctatt gccgccaaat ctgctgaata
    87121 atggcacaac ggagcaaagt gatgtaacag aggttactga ggcacacctg tcactcgatg
    87181 atgcgggcgg cacggcgctg gaggagatgg aaaagcaaat gatccgcgag gcgctttcac
    87241 gtcataacag caagaagcaa gttgctgatg aactgggcat cggcattgct acgctctatc
    87301 gcaagattaa gaaatatgag ttgttaaaca cataacatca gtgaatccgg cctgaattca
    87361 ggccggatat ccttttactt cgttttctcg caatccagaa ctgtatttac aatcatctga
    87421 taccccgtgc agcgacaaag atttcccgcc agtccgcgac gaatttccgt aatggttaat
    87481 ggcttctcgc gtggtttcgc cagcattgcc gtggtagcca taatcaggcc aggcgtacaa
    87541 aacccgcact gcactgcgcc ggatttcgca taagcctgct gaacatgaga aagttttccg
    87601 cctttcgctt caccttccag cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat
    87661 aagcaactgt ctattgctgt gccgtcgacc agcaccgtac aggcaccaca ttcacccacg
    87721 cagcaccctt gtttgacact tagcagccct tgttcgcgga gtaattccga gagcggcgtg
    87781 cctggtacgg catgaagctg aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt
    87841 tcgctgtgat tcattgcaat tttccccccg ccgcggcgac ggcttcgcta atcacttttt
    87901 tggtcatcgt ctggatgaga tgcagacgaa actctttact ggcccgccat gaagaacgcg
    87961 gggcgacatc ttgcaggaca gattcgctga tagcttccag cgtttgcagg tttaatggcg
    88021 cattttgtgc agtctgttcg gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa
    88081 atgccaggcg taattcgctg aaattgccgt tatccagtcg gcaatgtgcg gcacagccaa
    88141 tcgttgaaat atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccacgtgtt
    88201 ctttcggctg gggcggaaaa tgaaaggcga caaggatttc gtcatgctca agagacactt
    88261 tgcccggccc ggtgtgaaag ccattaatcg ggacgaaacg aacaccgcgt ggggagtgga
    88321 tctccagttt cgcgtcataa atgagcgttg gcgtggcaga atctgcgctg gtggcaccgt
    88381 tgcaaatatt tccaccgtag gtagcgacgt tacggatctg cggtccagca atggacgagg
    88441 ccgcagcaca taacgccggg agatgacgtt gagttataga atcttctatt agctgggtaa
    88501 atgtcgttgc agagccgata cgtagcgagc catcttccgc cagcgtaatt ccccgcagct
    88561 ccgccagatt atgaatatca acaatatggc gataacggtc attgtgatgg tggagctgaa
    88621 tcagtacgtc agtgccaccg gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga
    88681 tggcatcggc aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct
    88741 caaatcaatc ctgccagatg gaactcttca tataaccgtt ttggcgtcag cggcagtgta
    88801 ttgattgcaa caccggtagc catcttcacc gcgttacgaa tagcagcggc aacaggaatt
    88861 attggtggct cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg
    88921 aacgcgcttt ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg
    88981 gggttacgga ccacgccgct tttcgcatcg atgatcatct cttcaaatag cgcccagcca
    89041 atgcccattc ccattccgcc gtgtacctga ccttctgcca gtagcggatt aagaatatgg
    89101 cccgaatcat gaacgttgag gatgcggttg atggtgactt tgcacagcgc aatatcgacc
    89161 gtcagatcaa caaaggtaca gccgaacgcc ggtgggttag tggtggtttt gatggagctt
    89221 tcagcagaga gctgcccgcc gcgttcaggg tggtagaaag cgtccatcgc caaatctttt
    89281 aacgacatta acggctcttc cggtcgttca accagcacga tatggccttt tatcagggtc
    89341 agattcatcg ctgactgatg tagcatgact gcggcgtgag cgatgatttt ctcttttaat
    89401 aacagtgccg cactgcgcag cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg
    89461 ccgggatcga acggcgtaac gtcggtatct tgtgttgaaa taacgcgaac gtcgctgacc
    89521 ggaaccccca cggtttctgc caccatttgc gagaagacgg tgtcggcacc ctgaccgatt
    89581 tccgtcgcgc cgctttgcac gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct
    89641 gctatttcta cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg
    89701 ccgcggcgta aattgccttg ctggttctgg cactctgcac ggcgtttttc ccattcaaag
    89761 attttccggc ctttttcaag acactccggc aaccctgcgc tgtaaatacg tttgcccgtg
    89821 agcggattag catctccttc gcgtgaggcg ttgcgtaaac gaatttcaac aggatcaata
    89881 cctaacgctg tcgcggcgtc atcaagcata gactcaacgg caaatacgac ttgtggcgcg
    89941 ccataaccac gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa
    90001 gcgtaggcac aacgaggata aaggtaagcg actttatttc cccctgcaga agcgatggag
    90061 tgcccgtgag atacataagc gccggtgtta gacagaacat ccagactata acctttcaat
    90121 gttccgtcgc ggttcacgcc catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt
    90181 gcgaggaaac actcttcacg gctaagggaa actttcaccg gaatgccgcc aagcttgctg
    90241 gtcaggaatg ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg
    90301 acaaatggtt tgatgactcg tacgcatgac cagggaatat ccagcgcctg accaaccacg
    90361 cggcgaacaa tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc
    90421 atccacgcca gcgatgttac gctttccatg tgacaatgtt gaataacggg ggtctgatag
    90481 tgcccctgta cctggtagtc ggcggcatcg attgtttgtt ggacattgcc cgtcgacatc
    90541 gtgctttgtt tcagtaaatt gccgccgtta tggattggtg cagcgtcctc tgccagcgcc
    90601 gcttctggcg tggtgataac gggtaattct tcccactcaa tgctgaccaa ttgcgccgct
    90661 ttttctgccg tgagttcatc gcgggccacg acgatggcaa cggcgtcacc atgatgacga
    90721 acatggcgag ttagcagtgc gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccat
    90781 gcatgcccag ctgtagcgaa tgggatatca ggcacatctt cccaggtaaa aatcgccagt
    90841 acgcctggta aacttctggc ttgttcatca ttaatactta cggcataacc atgtgcgata
    90901 gggctacgta catatttcgc ataacacatg cccgccataa cataatcgtc agtatatcgt
    90961 gcccgcccgg tgaccttagc aatggcatca acgcgcatgc atgattcacc cgtagcggtt
    91021 gcttcccgcg cttccataga aatcccctcg atttgaacat ttggttctta tggcaaagat
    91081 ttagtctaag caggcaagat ttacgccaga aacggcgata agcgttaagg gaggaaggaa
    91141 gtgtgatgat gttaaaaaaa cacagatatg atacgaaaca ggaccctgaa taggggtagt
    91201 gatacgtatc aatatgagtg gcgataaaat caatgtgata aattaggatt attacgcatc
    91261 aggatgggaa atagggtaaa aaagtcagga acttcgatac ctctacagtg aatattttgc
    91321 tgatgatact tccctttgcc aaatgaaatt aacctctttc tctattacat cgtttttcca
    91381 gaagcaagag attgggtatc aagaggctgg ctgctatgat atggcgtctt gttttttaag
    91441 cgaggaaaga ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg
    91501 ttatcggttg gactgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg
    91561 tattccggct ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc
    91621 acgggaacca gcgtaaaaga gctggcgcga ctgaacggca tttccccccc ttacaccatt
    91681 gaagttggtc agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca
    91741 accgccaaat caacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatct
    91801 tcctggccgc cagtagggca acgttgttgg ttatggccaa cgacagggaa agttatcatg
    91861 ccgtattcga cagcagatgg cggcaataaa gggattgata tctcagctcc acggggtaca
    91921 cctatttacg ccgcgggtgc aggaaaggtg gtgtatgtgg gcaaccagct gcgtggctac
    91981 ggtaatctca tcatgattaa acacagtgaa gattacatta cggcttacgc ccataatgac
    92041 acgatgctgg taaataatgg gcaaagcgtg aaggctgggc aaaaaatcgc caccatgggg
    92101 agcacggatg cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt
    92161 gatccgctac gttacttgcc gcctcagggc agcaagccaa aatgctgatg gcgaattaat
    92221 cagcagtcag cagcgcggca cttgctaaga agagcgtaag gtttataatg ccttacgcat
    92281 ctcgaagcgg gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc
    92341 gattcccttc gcccgctcca atttatcttc acctcatcaa tctgccattt ttgctgtatc
    92401 tgattttcta tattatctta attcaatcgc ttcagacttt tcgcaatctc tgtaacactc
    92461 ccccattttt ttgtaaataa aaagcgttta cgcaatgcaa tccttttgta acaacccagg
    92521 taaaatacct tattttcaag ttgttaaata agtggttatc tacgctttta acaaacgatt
    92581 gatggaatat tttatgaaag ggaaatctgc attaactctt ctgctcgctg gtatattttc
    92641 gtgcggcaca tgccaggcga caggggcaga agtgacgagt gaatctgtat ttaacattct
    92701 taattccacg ggggcagcaa cagataaaag ttatttatcg ttaaatccag ataagtatcc
    92761 taattatcgt cttctgattc actcagccaa attacaaaat gaaataaaga gccactatac
    92821 caaagacgaa attcaaggat tgctaacatt aacagaaaac acacgaaagt taacgttgac
    92881 ggagaaaccc tggggaacgt ttattcttgc ttctactttc gaagatgaca aaacagcggc
    92941 agaaacccac tatgatgctg tctggctgag ggatagttta tggggctata tggcattagt
    93001 atcagaccag ggaaatagtg tagccgcaaa aaaggttctg ctcaccctgt gggattatat
    93061 gtctacgccc gaccagatta agcggatgca ggacatcatt agcaacccaa aacgactgga
    93121 tggtattccc gggcaaatga atgctgtgca tatttgacag caactccccc gtgatggcag
    93181 atgtgcagga agaaggcaag ccgcagctgt ggaaccataa acaaaatgat gcgttaggtc
    93241 tttatcttga tctccttata caagcaatca atactggtac tattaacgct gaagattggc
    93301 aaaaaggtga ccgactaaaa tcggttgcgc tgctgattgc ttatctggat aaagccaatt
    93361 tctatgttat ggaagattcc ggagcgtggg aggaagatgc ccgattaaac acatcttccg
    93421 ttgcactggt gacctctgga ctggaacgtt tgtcaaacct actctcgaag aaagactctg
    93481 tttttgtctc tgatttacta cgcgaagcta aagtcaatga actggatgaa actctttcaa
    93541 caacgaggtt gaaccacctc atagataaag gttatgaaag aataacgtta cagcttgatt
    93601 tagggggaga atccccaggt tatctggaaa aagataaaca ttacagagaa gccgatgcgg
    93661 ctttacttaa tgttatttat cctgcaaatc tttcaaagat aaacaccaga aga
//
LOCUS       DBFIFS010000002        81711 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.002, whole genome shotgun sequence.
ACCESSION   DBFIFS010000002 DBFIFS010000000
VERSION     DBFIFS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.002"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            42..209
                     /gene="yhfL"
                     /locus_tag="N7A07_000089"
     CDS             42..209
                     /gene="yhfL"
                     /locus_tag="N7A07_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU2474842.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            505..1842
                     /gene="frlA"
                     /locus_tag="N7A07_000090"
     CDS             505..1842
                     /gene="frlA"
                     /locus_tag="N7A07_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU2474843.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1863..2885
                     /gene="frlB"
                     /locus_tag="N7A07_000091"
     CDS             1863..2885
                     /gene="frlB"
                     /locus_tag="N7A07_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU2474844.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2936..3763
                     /gene="frlC"
                     /locus_tag="N7A07_000092"
     CDS             2936..3763
                     /gene="frlC"
                     /locus_tag="N7A07_000092"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU2474845.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3763..4548
                     /gene="frlD"
                     /locus_tag="N7A07_000093"
                     /pseudo
     CDS             3763..4548
                     /gene="frlD"
                     /locus_tag="N7A07_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4648..5379
                     /gene="frlR"
                     /locus_tag="N7A07_000094"
                     /gene_synonym="yhfR"
     CDS             4648..5379
                     /gene="frlR"
                     /locus_tag="N7A07_000094"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU2474846.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5510..6514)
                     /gene="trpS"
                     /locus_tag="N7A07_000095"
     CDS             complement(5510..6514)
                     /gene="trpS"
                     /locus_tag="N7A07_000095"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU2474847.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6507..7265)
                     /gene="gph"
                     /locus_tag="N7A07_000096"
     CDS             complement(6507..7265)
                     /gene="gph"
                     /locus_tag="N7A07_000096"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU2474848.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7258..7935)
                     /gene="rpe"
                     /locus_tag="N7A07_000097"
     CDS             complement(7258..7935)
                     /gene="rpe"
                     /locus_tag="N7A07_000097"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU2474849.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7953..8789)
                     /gene="dam"
                     /locus_tag="N7A07_000098"
     CDS             complement(7953..8789)
                     /gene="dam"
                     /locus_tag="N7A07_000098"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU2474850.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8896..10182)
                     /gene="damX"
                     /locus_tag="N7A07_000099"
     CDS             complement(8896..10182)
                     /gene="damX"
                     /locus_tag="N7A07_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU2474851.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10274..11362)
                     /gene="aroB"
                     /locus_tag="N7A07_000100"
     CDS             complement(10274..11362)
                     /gene="aroB"
                     /locus_tag="N7A07_000100"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU2474852.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11419..11940)
                     /gene="aroK"
                     /locus_tag="N7A07_000101"
     CDS             complement(11419..11940)
                     /gene="aroK"
                     /locus_tag="N7A07_000101"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU2474853.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12341..13579)
                     /gene="hofQ"
                     /locus_tag="N7A07_000102"
     CDS             complement(12341..13579)
                     /gene="hofQ"
                     /locus_tag="N7A07_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU2474854.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13491..13895)
                     /gene="hofP"
                     /locus_tag="N7A07_000103"
     CDS             complement(13491..13895)
                     /gene="hofP"
                     /locus_tag="N7A07_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU2474855.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13885..14325)
                     /gene="hofO"
                     /locus_tag="N7A07_000104"
     CDS             complement(13885..14325)
                     /gene="hofO"
                     /locus_tag="N7A07_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU2474856.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14309..14848)
                     /gene="hofN"
                     /locus_tag="N7A07_000105"
     CDS             complement(14309..14848)
                     /gene="hofN"
                     /locus_tag="N7A07_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU2474857.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14832..15626)
                     /gene="hofM"
                     /locus_tag="N7A07_000106"
     CDS             complement(14832..15626)
                     /gene="hofM"
                     /locus_tag="N7A07_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU2474858.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEPWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15746..18298
                     /gene="mrcA"
                     /locus_tag="N7A07_000107"
     CDS             15746..18298
                     /gene="mrcA"
                     /locus_tag="N7A07_000107"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU2474859.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18463..19023)
                     /gene="nudE"
                     /locus_tag="N7A07_000108"
     CDS             complement(18463..19023)
                     /gene="nudE"
                     /locus_tag="N7A07_000108"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU2474860.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19343..21478
                     /gene="igaA"
                     /locus_tag="N7A07_000109"
     CDS             19343..21478
                     /gene="igaA"
                     /locus_tag="N7A07_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU2474861.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21543..22211
                     /gene="yrfG"
                     /locus_tag="N7A07_000110"
     CDS             21543..22211
                     /gene="yrfG"
                     /locus_tag="N7A07_000110"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU2474862.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22222..22623
                     /gene="hslR"
                     /locus_tag="N7A07_000111"
     CDS             22222..22623
                     /gene="hslR"
                     /locus_tag="N7A07_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU2474863.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22648..23526
                     /gene="hslO"
                     /locus_tag="N7A07_000112"
     CDS             22648..23526
                     /gene="hslO"
                     /locus_tag="N7A07_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU2474864.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23589..25313)
                     /gene="yhgE"
                     /locus_tag="N7A07_000113"
     CDS             complement(23589..25313)
                     /gene="yhgE"
                     /locus_tag="N7A07_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU2474865.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25692..27314
                     /gene="pckA"
                     /locus_tag="N7A07_000114"
     CDS             25692..27314
                     /gene="pckA"
                     /locus_tag="N7A07_000114"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU2474866.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27390..28742)
                     /gene="envZ"
                     /locus_tag="N7A07_000115"
     CDS             complement(27390..28742)
                     /gene="envZ"
                     /locus_tag="N7A07_000115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU2474867.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28739..29458)
                     /gene="ompR"
                     /locus_tag="N7A07_000116"
     CDS             complement(28739..29458)
                     /gene="ompR"
                     /locus_tag="N7A07_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU2474868.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29686..30162
                     /gene="greB"
                     /locus_tag="N7A07_000117"
     CDS             29686..30162
                     /gene="greB"
                     /locus_tag="N7A07_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU2474869.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30259..32580
                     /gene="yhgF"
                     /locus_tag="N7A07_000118"
     CDS             30259..32580
                     /gene="yhgF"
                     /locus_tag="N7A07_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU2474870.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIITDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            33018..33245
                     /gene="feoA"
                     /locus_tag="N7A07_000119"
     CDS             33018..33245
                     /gene="feoA"
                     /locus_tag="N7A07_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU2474871.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33262..35583
                     /gene="feoB"
                     /locus_tag="N7A07_000120"
     CDS             33262..35583
                     /gene="feoB"
                     /locus_tag="N7A07_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU2474872.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35583..35819
                     /gene="feoC"
                     /locus_tag="N7A07_000121"
     CDS             35583..35819
                     /gene="feoC"
                     /locus_tag="N7A07_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU2474873.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            36022..36900
                     /gene="rpnA"
                     /locus_tag="N7A07_000122"
     CDS             36022..36900
                     /gene="rpnA"
                     /locus_tag="N7A07_000122"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU2474874.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYPDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36927..37697)
                     /gene="bioH"
                     /locus_tag="N7A07_000123"
     CDS             complement(36927..37697)
                     /gene="bioH"
                     /locus_tag="N7A07_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU2474875.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37735..38418
                     /gene="gntX"
                     /locus_tag="N7A07_000124"
     CDS             37735..38418
                     /gene="gntX"
                     /locus_tag="N7A07_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU2474876.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38477..39052
                     /gene="nfuA"
                     /locus_tag="N7A07_000125"
     CDS             38477..39052
                     /gene="nfuA"
                     /locus_tag="N7A07_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU2474877.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39413..40729
                     /gene="gntT"
                     /locus_tag="N7A07_000126"
     CDS             39413..40729
                     /gene="gntT"
                     /locus_tag="N7A07_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU2474878.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40774..42858)
                     /gene="malQ"
                     /locus_tag="N7A07_000127"
     CDS             complement(40774..42858)
                     /gene="malQ"
                     /locus_tag="N7A07_000127"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU2474879.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42868..45261)
                     /gene="malP"
                     /locus_tag="N7A07_000128"
     CDS             complement(42868..45261)
                     /gene="malP"
                     /locus_tag="N7A07_000128"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU2474880.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45873..48578
                     /gene="malT"
                     /locus_tag="N7A07_000129"
     CDS             45873..48578
                     /gene="malT"
                     /locus_tag="N7A07_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU2474881.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48645..48923
                     /locus_tag="N7A07_000130"
     CDS             48645..48923
                     /locus_tag="N7A07_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU2474882.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            48914..49399
                     /locus_tag="N7A07_000131"
     CDS             48914..49399
                     /locus_tag="N7A07_000131"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2474883.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49449..50477)
                     /gene="rtcA"
                     /locus_tag="N7A07_000132"
     CDS             complement(49449..50477)
                     /gene="rtcA"
                     /locus_tag="N7A07_000132"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU2474884.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50481..51707)
                     /gene="rtcB"
                     /locus_tag="N7A07_000133"
     CDS             complement(50481..51707)
                     /gene="rtcB"
                     /locus_tag="N7A07_000133"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU2474885.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            51895..53493
                     /gene="rtcR"
                     /locus_tag="N7A07_000134"
     CDS             51895..53493
                     /gene="rtcR"
                     /locus_tag="N7A07_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU2474886.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(53475..54233)
                     /locus_tag="N7A07_000135"
     CDS             complement(53475..54233)
                     /locus_tag="N7A07_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU2474887.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(54250..55080)
                     /gene="glpG"
                     /locus_tag="N7A07_000136"
     CDS             complement(54250..55080)
                     /gene="glpG"
                     /locus_tag="N7A07_000136"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU2474888.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(55125..55451)
                     /gene="glpE"
                     /locus_tag="N7A07_000137"
     CDS             complement(55125..55451)
                     /gene="glpE"
                     /locus_tag="N7A07_000137"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU2474889.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55641..57146
                     /gene="glpD"
                     /locus_tag="N7A07_000138"
     CDS             55641..57146
                     /gene="glpD"
                     /locus_tag="N7A07_000138"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU2474890.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(57361..57963)
                     /locus_tag="N7A07_000139"
     CDS             complement(57361..57963)
                     /locus_tag="N7A07_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2474891.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(57963..58955)
                     /locus_tag="N7A07_000140"
     CDS             complement(57963..58955)
                     /locus_tag="N7A07_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2474892.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(58981..60489)
                     /locus_tag="N7A07_000141"
     CDS             complement(58981..60489)
                     /locus_tag="N7A07_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2474893.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(60614..63061)
                     /gene="glgP"
                     /locus_tag="N7A07_000142"
     CDS             complement(60614..63061)
                     /gene="glgP"
                     /locus_tag="N7A07_000142"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU2474894.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(63080..64513)
                     /gene="glgA"
                     /locus_tag="N7A07_000143"
     CDS             complement(63080..64513)
                     /gene="glgA"
                     /locus_tag="N7A07_000143"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU2474895.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(64513..65808)
                     /gene="glgC"
                     /locus_tag="N7A07_000144"
     CDS             complement(64513..65808)
                     /gene="glgC"
                     /locus_tag="N7A07_000144"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU2474896.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65826..67799)
                     /gene="glgX"
                     /locus_tag="N7A07_000145"
     CDS             complement(65826..67799)
                     /gene="glgX"
                     /locus_tag="N7A07_000145"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU2474897.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67796..69982)
                     /gene="glgB"
                     /locus_tag="N7A07_000146"
     CDS             complement(67796..69982)
                     /gene="glgB"
                     /locus_tag="N7A07_000146"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU2474898.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(70255..71358)
                     /gene="asd"
                     /locus_tag="N7A07_000147"
     CDS             complement(70255..71358)
                     /gene="asd"
                     /locus_tag="N7A07_000147"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU2474899.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            71550..72143
                     /gene="yhgN"
                     /locus_tag="N7A07_000148"
     CDS             71550..72143
                     /gene="yhgN"
                     /locus_tag="N7A07_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU2474900.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(72189..73484)
                     /locus_tag="N7A07_000149"
     CDS             complement(72189..73484)
                     /locus_tag="N7A07_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU2474901.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(73391..75515)
                     /locus_tag="N7A07_000150"
                     /pseudo
     CDS             complement(73391..75515)
                     /locus_tag="N7A07_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(75655..76995)
                     /gene="gntU"
                     /locus_tag="N7A07_000151"
     CDS             complement(75655..76995)
                     /gene="gntU"
                     /locus_tag="N7A07_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU2474902.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76999..77526)
                     /gene="gntK"
                     /locus_tag="N7A07_000152"
     CDS             complement(76999..77526)
                     /gene="gntK"
                     /locus_tag="N7A07_000152"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU2474903.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(77665..78660)
                     /gene="gntR"
                     /locus_tag="N7A07_000153"
     CDS             complement(77665..78660)
                     /gene="gntR"
                     /locus_tag="N7A07_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU2474904.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(78884..79579)
                     /gene="yhhW"
                     /locus_tag="N7A07_000154"
     CDS             complement(78884..79579)
                     /gene="yhhW"
                     /locus_tag="N7A07_000154"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU2474905.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(79702..80739)
                     /gene="yhhX"
                     /locus_tag="N7A07_000155"
     CDS             complement(79702..80739)
                     /gene="yhhX"
                     /locus_tag="N7A07_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU2474906.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            81073..81561
                     /gene="yhhY"
                     /locus_tag="N7A07_000156"
     CDS             81073..81561
                     /gene="yhhY"
                     /locus_tag="N7A07_000156"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2474907.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT        19732 a        22186 c        20475 g        19318 t
ORIGIN      
        1 tcaatgaata ttaatcaata tataatacat agggaatgta aatgaacaaa tttattaaag
       61 ttgcactggt aggtgcagta ctggctacgt taactgcatg tactggtcat attgaaaacc
      121 gtgataagaa ctgctcttac gactacctgc tgcacccagc aatttctatt tctaaaatca
      181 ttggcggttg cggtcctact gcacagtaag tctctgacag aaatcagcta acgctgattt
      241 aattgcctga cgccctcggg attacccgag ggcgtatttg tttctatatc cttcttgctc
      301 cccacccgta aaaatatttt cactttaatt tcaattggat aaccacattc atcagccaca
      361 gcgcgatcct gtgcgaaatt atgtgatctt cctccacatt acataacatc atatgttgtt
      421 atattcatca tgcattgtca tgtcaccttt taaatgactg caaactctcc cctacaacgt
      481 gttggacaag aaaaaggtat cgctatggga agccaggaac tccaacgcaa gctcggattt
      541 tgggccgttc ttgcaatcgc cgtcgggaca accgtcggct ccggtatttt tgtatctgtg
      601 ggtgaagtgg caaaagcggc gggcacgccg tggctgacgg tgctcgcatt tgtcatcggc
      661 gggttaattg tgatcccgca aatgtgcgta tatgcggaac tatccaccgc ttatccggaa
      721 aatggcgcag attatgttta tctgaaaaat gccggaagcc gaccgctggc tttcctctcc
      781 ggctgggcca gcttctgggc caacgatgcg ccgtcattgt cgattatggc gctggcgatt
      841 gtcagcaatc ttggcttttt aacgcctatc gatccgttgc tcggtaaatt tatcgccgcc
      901 ggattaatta tcgcctttat gctgctgcac ctgcgctccg ttgaaggcgg cgcagcgttt
      961 cagacgctaa ttaccatcgc caaaattatc ccgttcacta tcgtcattgg tcttgggatc
     1021 ttctggttta aagcggagaa ttttgccgcc cctgctacca ctgcgattgg cgcaacgggc
     1081 agctttatgg cgctgctggc ggggatctct gccaccagtt ggtcgtatac cggcatggcc
     1141 tctatctgtt atatgaccgg cgaaattaaa aaccccggca aaaccatgcc acgagcgctg
     1201 attggttcct gtctgctggt tctggtgctc tacaccctgc tggcgctggt gatttccggc
     1261 ctgatgccct tcgacaaact cgccaattct gaaacgccga tttccgacgc cctgacctgg
     1321 atccccgcac tcggcagcac cgctgggatc tttgttgcca tcacggcgat gatcgtcatt
     1381 cttggttcgc tttccagctg cgtgatgtac cagccgcggc tggaatacgc gatggcgaaa
     1441 gacaacctgt tcttcaaatg cttcggccat gtgcatccga aatacaacac gccggatgtc
     1501 tccatcattt tgcaaggggc gctggggatc ttcttcatct tcgtttccga tctcaccagc
     1561 ctgctgggct atttcaccct ggtaatgtgt ttcaaaaata ccctcacctt cggctccatc
     1621 atctggtgtc gtaaacgcga cgattacaaa ccgctgtggc gtactccggc tttcgggctg
     1681 atgacccccc tcgccattgc gtcaagcctc attctggtcg cctcaacctt tgtctgggca
     1741 ccgattcccg gccttatctg cgccgtcatc gttattgcca ctggtctgcc tgcttacgcc
     1801 ttctgggcga agcgtagccg ccagctcaac gctttgtcgt aatgttatct ggagaaaata
     1861 aaatgttgga tattgataaa agcactgtgg actttctggt caccgaaaat atggttcagg
     1921 aagtggaaaa agttctcagc catgacgttc cgctggtgca caccatcgtg gaagagatgg
     1981 tgaagcgcga catcgatcgt atttatttcg ttgcctgcgg atcgccactc aacgcggcgc
     2041 aaacggcgaa acatctggca gatcgctttt ccgatcttca ggtttacgcc atttccggct
     2101 gggagttctg cgataacacc ccatatcgcc tcgacgctcg ttgcgcggtg attggcgttt
     2161 ctgactacgg taaaaccgaa gaggtaatca aagcgctgga gctgggccgg gcctgcggcg
     2221 cactcactgc ggcgttcacc aaacgcgcgg atagcccgat tacctcggcg gcggaattta
     2281 gcattgatta tcaggccgac tgtatctggg aaattcatct gctgctctgc tacagcgtgg
     2341 tgctggagat gatcacccgc ctcgcgccgc acgcggaaat cggcaagatc aaaaacgatc
     2401 tcaaacagct gccgaatgcg ctcggttatc tggtacgcac ttgggaagaa aaaggccgcc
     2461 agcttggtga actggccagc cagtggccga tgatttacac ggttgctgca ggcccgctgc
     2521 gcccgctggg ttacaaagaa ggcatcgtga cgctgatgga atttacctgg acgcacggct
     2581 gtgtgattga gagcggtgag ttccgtcacg gcccgctgga gattgtcgaa ccgggcgttc
     2641 cgttcctgtt cctgctcggc aatgatgaaa gtcgccacac caccgaacgc gccattaact
     2701 ttgttaaaca gcgtactgac aacgtgatcg tcatcgatta cgccgcaatt tcgcaaggac
     2761 tgcacccgtg gctggcaccg ttcctgatgt tcgtgccaat ggagtggctc tgctactacc
     2821 tgtctattta caaagatcac aacccggatg aacgccgcta ttacggtggt ctggtggaat
     2881 attaatcctc tctcccggcc cggcaacggg ccggatttac gcaaggagtt acccgatgaa
     2941 aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacatgcat tccgtgatgc
     3001 aagcgagctg ggttacgatg gcatcgaaat ttggggcggc cgcccgcacg cgttcgcgcc
     3061 ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt atcagatgcc
     3121 gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc tgggcgatga
     3181 acatatgcgt cgtgaaagcc tggacatgat caagctggcg atggatatgg caaaagagat
     3241 gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc cgcctaacgt
     3301 tatctggggg cggctggcag agaacttaag cgaactgtgt gagtacgcgg aaaacatcgg
     3361 catggatctg atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt gtaatgctaa
     3421 tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca tggtcgacat
     3481 ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac tgggcgataa
     3541 attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt acattcccgg
     3601 cgaaggcaaa atgccgctgc gggaactgat gcgcgatatt attgatcggg gctatgaggg
     3661 atactgcaca gtggaactgg taaccatgta tatgaacgag cccagacttt acgcccgcca
     3721 ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag tgatgaaaac cctggcgaca
     3781 atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc tggcggtaat
     3841 gcggtgaacg tggcggtgta ctgcactcgc tacggtataa agccgggatg cattacctgg
     3901 gttggtgacg atgactacgg cacaaagctt aagcaggatc tcgccagcat gggcgtcgat
     3961 atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga actgcacgac
     4021 aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc cctgagtgaa
     4081 gaggattacg cctagctggc gcagtatgac attgtgcacg cggcaatctg gggacatgcg
     4141 gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga cgtctccgac
     4201 aagtgggaca gcccgctctg gcagacactg gtgccgcatc tcgattttgc ctttgcctcc
     4261 gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc ccgtggcgca
     4321 ggcgtggtga ttgtcacgtt gggtgaaaac ggcagtattg cctgggatgg cgtgcagttc
     4381 tggcgtcagg ctcctgaaca ggtgacggtt atcgacacca tgggtgccgg agattcgttc
     4441 attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc gatggcgcag
     4501 ggaacggcgt gcgcggcaca aactattcag taccacggtg cctggtaggc ataacgttgg
     4561 cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta taatccgtgt
     4621 aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc tcatcaactc
     4681 ctctacgcta ccgtccgcca gcggctgctg gatgatatcg cgcagggggt ttaccaggcc
     4741 gggcaacaga tccctaccga aaatgagctt tgtacacaat ataacgtcag ccgcattacc
     4801 attcgcaaag ccatcagcga cttagtggca gacggcgtac tgatccgctg gcagggcaaa
     4861 ggcacctttg tacaaagcca gaaggtcgaa aacgccctgc tgaccgtcag tggttttacc
     4921 gattttggcg tctcacaagg caaggcgacg aaagagaaag tgatcgagca agaacggatc
     4981 agcgccgcgc cgttttgcga aaagctcaat atccccggta acagcgaagt gttccatctt
     5041 tgccgggtga tgtatctcga taaagagccg ctatttattg atagttcgtg gatcccgctg
     5101 tcgcgttatc ctgactttga tgagatttac gtcgaaggaa gctccaccta tcagttattt
     5161 caggagcgtt ttgacacgcg agtggtcagc gacaaaaaga ccatcgatat ctttgccgcc
     5221 acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt tcgcatcagc
     5281 aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt ctgccgcgcc
     5341 aaccgcatca ccttaacaat tgataataaa agacattaac cgtaggccgg ataagatgca
     5401 ccagcatcgc atccggcgat gccggcgcac tgaattttac atcccgcacg ttcccctcac
     5461 cctaaccctc tccccaaagg ggcgagggga ccgtccgagc gttttgatat tacggcttcg
     5521 ccacaaaacc aatcgcttcg tacaccgctt ttagcgtacg ggaagcgtgc gcgctggctt
     5581 tttctgcgcc gtctttcatc acctgttgca ggaaggcttc atcgttgcgg aaacggtggt
     5641 aacgttcctg caattcagtc agcataccgg aaacggcatc agccacttcg cctttcagat
     5701 gaccatacat cttgccttcg aactgttttt ccagttccgg gatgctctgg cccgttaccg
     5761 cagaaaggat atccagcagg ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta
     5821 ccggcggctc gtcggagtcg gtgaccgcgc gtttgatttt cttcactacc gatttcggat
     5881 cttccagcag gccaataacg ttattgcggt tatcgtcaga cttggacatc ttcttggtcg
     5941 gctccagcag cgacattacg cgcgcgccgg atttcggaat aaacggctcc ggcaccttaa
     6001 agatatcgcc gtacagcgcg ttgaaacgct gggcgatatc acggctcagt tccaggtgct
     6061 gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg tccgctgcca
     6121 tcagcaccgg atagtcaaac agaccagcgt tgatgttctc ggcataacgc gcagatttat
     6181 ctttaaactg cgtcatgcgg ctcagttcgc cgaagtaggt atagcagttc agtgcccagc
     6241 ctaactgcgc atgttccggt acgtgggact gaacaaaaat ggtgcttttc tcaggatcga
     6301 taccacaagc cagatacaag gccagcgtat ccagcgtcgc tttacgcagc ttctgtgcat
     6361 cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg tagtcatcct
     6421 gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatagtcaat tcacctgagg
     6481 gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt ttcgctatgc
     6541 ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc aggctggctg
     6601 agatcgatag cctcgccgta gttatatccg taggttaagc caactgatgg gcaacctgcc
     6661 gcttttgctg cctgaatatc attgcgtgag tcgccaacaa acagcatctg ttgtggagca
     6721 attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt gttttgcaca
     6781 tcatcaccac caatcaccac gctgaagtat ttggcgatat ctaaggcttc gagcagcggc
     6841 gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg gcaggccttt agcctgcaac
     6901 gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca
     6961 tagtaacgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg aatgtcgtca
     7021 tcaacgggcg gtttacccat tgttttacgc agagtcgcac gttcctgacg cgcccaggtc
     7081 aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac gcgttcttca
     7141 cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc aagaccggga
     7201 gcactgtcca ccagcgtacc gtcgagatca aaagcgacgc cgcgaatatc ttcaaactta
     7261 ctcatgactt acctttgcca gttcactgcg catttcatca atgacttttt tgtagtctgg
     7321 ctggtcgaag attgccgaac cggcaacgaa catatccgcg cccgccgcgg cgatttcgcc
     7381 aatgttgttc accttcacgc caccgtccac ttccagacga atgtcaaagc cagactcgtc
     7441 gatacgacga cgtacttcgc gcagtttatc cagtgtttga ggaatgaaag attgaccgcc
     7501 gaaaccaggg ttgacggaca tcagcaggat cacatccagc ttatccatca cgtaatccag
     7561 atagctcaga ggtgtcgccg ggttaaatac cagacccgct ttacagccat tttctttaat
     7621 cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa tgatgctggc
     7681 accagcggca gcgaaatcag gcacaatgcg atcgaccggt ttcaccatca ggtgtacgtc
     7741 gataggggcg gtaatgccgt agttacgcaa ggatttcagc accattggcc caatcgtcag
     7801 attgggaacg tagtggttat ccatgacgtc aaaatgcacg acatcagcgc cagctgccag
     7861 ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg agggggcaat
     7921 caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga aacgactcct
     7981 ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt gctgcttata
     8041 ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg cgttaacatc
     8101 gtatcgtgat tggagatcag cacaggaata tggcgctcaa ccagaccttc ggcgatctcc
     8161 gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg
     8221 gtcgcagaca gcggtgcata aggtggatcg caatagacga cagatgcatc atctgcgcgc
     8281 gccatgctat cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag
     8341 tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg cacattaaac
     8401 tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa atataaaaac
     8461 agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa ctgatagtaa
     8521 acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac gtactcatca
     8581 gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc ggcaaggata
     8641 taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg ctcaaccaga
     8701 cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt gccccctgcc
     8761 cacttcaaaa aagcgcgatt tttcttcatg ctgactaact aattacacct tctccggctg
     8821 tggagaaagc tccgacagca tccagcgctt caggcaactc ccgcaacgat aaaccattgc
     8881 gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg
     8941 gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct cttctttcga
     9001 agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg tttcatagac
     9061 aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt agttagagga
     9121 actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac ccacattacc
     9181 tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac
     9241 tggagccgta gtcgccgtct cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt
     9301 gctcgccaca ggagcagctg gttcagtacg cttcggcgtc tgtgctaccg gcttcggttc
     9361 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gagctggacg
     9421 cgtagtggcc ggacgttcag cagtttgcgt tttcgccgtg tcacgcgatg cattgccatt
     9481 gcgtactggc gcgacagttg ctggttcagt cggcaatgta gaattgaccg ccacattgtt
     9541 caactgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
     9601 acgttgttga ccatccgttg ccgccggggt ttgcccttga gtcggcgtag aagagatcgg
     9661 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
     9721 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
     9781 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
     9841 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
     9901 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
     9961 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    10021 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    10081 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    10141 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    10201 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    10261 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    10321 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    10381 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    10441 gcggcccatt gaccggtaac ccagcccgcg tgagcagagt tataatacgc tgcgtttcgg
    10501 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    10561 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    10621 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    10681 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    10741 acaacgcatc cagattctct tccagccagt taaaaaacgt accgtcaaga ataatgccgt
    10801 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    10861 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    10921 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    10981 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    11041 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    11101 acgccgtaaa gacggtatcg agtacagcca ggcttttata ctgctcgccg tcaggaagga
    11161 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    11221 gaggagccag ggtttcgttg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    11281 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    11341 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    11401 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    11461 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    11521 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    11581 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atcgtcgttt caagataaac
    11641 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    11701 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    11761 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    11821 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    11881 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    11941 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagctccg
    12001 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    12061 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    12121 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    12181 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaaggcagg cagcgaagag
    12241 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    12301 aacacgttca gcgtaaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    12361 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    12421 attgcccgaa ccagggaatg tcgccaagca acggcacgct atcctgaccc gatttatttt
    12481 tacgggtaaa aatgccgccc agcgccaacg tttctccgct tttgacctcg acctgcgttt
    12541 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    12601 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    12661 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    12721 tggaaacctg atatggaatt tcgctcccct gtttaatgct ggcaggctga agatgtgagg
    12781 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    12841 caagatccag caaacgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    12901 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    12961 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    13021 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    13081 gggcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    13141 tcatactccc tttggcactc agtagcttct ccccagcttt cgccagctct ccggcgtcgg
    13201 cgtattgcag agtaatattg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    13261 ccgcttcctg acgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    13321 tgccctcctg gcgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    13381 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac accaccaggt
    13441 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    13501 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    13561 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    13621 cagtttgtcc cggtacccag cgttagtacg tctagcgtca gctgtaaaat tgtccagccg
    13681 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    13741 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    13801 atccggcata gatcttccgg cggtttaaaa gggtcacgca taccggttaa aaggcacaat
    13861 gcaatacctg ccaacaacca gcgtttaacc ctcatgcggc gtctccagtt gtagggtgaa
    13921 caaaagatca tcaccttcca cgcttaatga aaaacggctc acgctgacgt tgcgctctgc
    13981 cagccgtgta aatgccgacg gcactgcttc ccacagcgtt ttcaacgcca actcgcctcc
    14041 ctgcgcggat ggatgccaga aaaccagttg tgcgccgggc aactgaaaat ccagtggcga
    14101 aaagggcagc gttttttctt cgctgaaggg agtggtgtca accaggcgat acagtgtggc
    14161 ccaatgctga tgatgacttg cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt
    14221 cgatgacaga aaaatgagcg taactaacat cagcaaccag aatgcccagc aaaactggcg
    14281 gaggcggggt gatgtggcga accaccagtc aaagaacatg ttcatcgcta accttccttg
    14341 ttaactgata ctcaaattgc cagcgtccct gcgcatcctg ctgcgtggcc ccccgctgat
    14401 tgagatgaaa agatgcatcc tggcggagtg acgtttctag tgcgtttaac gcggtaatgc
    14461 ttgttgtaag ccccttgatc tccagcgttc cctgctgcca gcttatcgtt gtcagccagg
    14521 cgtgctcggg taaaagcgcc gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt
    14581 gtcgctggcg ctgcctttgc aaacgctgct cacgaatttg ttggcgctcc agcaaacgtg
    14641 gtttggttat ctgtaagctg cgggcgagtt gttgttcccc ctgaagcaaa acggcgtcta
    14701 cgcgagcttc ggcgctgctt gtcagacgca gtattagcgt tatcccgacg gccagcagca
    14761 gaggcgcaac gaacatcagc aaccagaaac gaagaaaagc ggtccggcgt tgctgtcgcc
    14821 agggcaaaaa attaattggc gggttcatca gtacgccttc ccaagcgcca gcccagcgcg
    14881 atggcaaagt ctccacctgg cggcggtaac ggcggctgac gaacagaaac ggcctcccaa
    14941 ggatcaaatc cgccttcgcc acatatcgcg acgctttcgg gatcaacgga taacgctgcc
    15001 gccagctcct tcgcgctagt catccccacc gccagtttgc gcccccagcg atagcgcgtc
    15061 gcccacagcc acggttcgtt atcacgccag gccagacatt gctgatgaga aggtaaaaaa
    15121 ggcaggaatc gctgtaatgc actggcatcc ggggtgatcg cactaacatg aacacgcaac
    15181 ctttctgcca aagtaagcag cgttgccagc tctttgcttt gcgcggcagt cacgttatag
    15241 gcgggactga gtgagtcttc gctgtaatcg aagcgcaggg agtccggatc catatccagc
    15301 tcgcgagcca tcgtcccgga cagccaggcc gtttgctccc gctcaccgag ggacatcgac
    15361 gggcgcggaa atgaccgctg taatgtgcga ctggcgggaa acgccaacat gatgtgatgg
    15421 cgctgcggca gttcgcgact ccacggtaac aacgttttag ccagccgctg cgcatcaaca
    15481 atccgcccat ctttgataat gtcgtgcgcc agcggcaacc gccaccagcg ttgcaaaaag
    15541 cattcttttg cgccccgcac aatagcaacc gctaccgctt cttgctgttg taaatgcaaa
    15601 ccaatttgcc agatcttaaa tgccattgtg atgatctcct tatcacccgt cactctgacg
    15661 ggtatatcaa tgcgtctggc ttgcctttat actaccgcgc gtttgtttat aaactgccca
    15721 aatgaaacta aatgggaaat ttccagtgaa gttcgtaaag tattttttga tccttgcagt
    15781 ctgttgcatt ctgctgggag caggctcgat ttatggccta taccgctaca tcgagccaca
    15841 actgccggat gtggcgacat taaaagatgt tcgcctgcaa attccgatgc agatttacag
    15901 cgccgatggc gagctgattg ctcaatacgg tgagaaacgt cgtattccgg ttacgttgga
    15961 tcaaatcccg ccggagatgg tgaaagcctt tatcgcgacg gaagacagcc gcttctacga
    16021 gcatcacggc gttgacccgg tggggatctt ccgtgcggca agtgtggcgc tgttctccgg
    16081 ccacgcgtca caaggggcaa gtaccattac ccagcagctg gcgagaaact tcttcctcag
    16141 tccagaacgc acgctgatgc gtaagattaa ggaagtcttc ctcgcgattc gcattgaaca
    16201 gttgctgacg aaagacgaga tcctcgagct ttatctgaac aagatttacc ttggttaccg
    16261 cgcctatggt gtcggtgctg cggcacaagt ctatttcgga aaaacggtcg accaactgac
    16321 gctgaacgaa atggcggtga tagccgggct gccgaaagcg ccttccacct tcaacccgct
    16381 ctactcgatg gatcgtgccg tcgcgcggcg taacgtcgtg ctatcgcgga tgctggatga
    16441 aggatatatc acccagcaac agttcgatca gacacgcact gaggcgatta acgctaacta
    16501 tcacgcgccg gagattgctt tttctgcgcc gtacctgagc gaaatggtgc gccaggagat
    16561 gtataaccgt tatggcgaaa gtgcctatga agacggttat cgcatttaca ccaccatcac
    16621 ccgcaaagtg cagcaggccg cgcagcaggc ggtacgtaat aacgtgctgg actacgacat
    16681 gcgccacggc tatcgcggcc cggcaaatgt gctgtggaaa gtgggcgagt cggcgtggga
    16741 taacaacaag attaccgata cgctgaaggc gctgccgacc tatggtccgc tgctgcctgc
    16801 cgcagtcacc agcgccaatc ctcaggaagc gacggcaatg ctggcggacg ggtcgaccgt
    16861 cgcattgagt atggaaggcg ttcgctgggc gcgtccttac cgttcggata ctcagcaggg
    16921 accgacgccg cgtaaagtga ccgatgttct gcaaacgggt cagcaaatct gggttcgtca
    16981 ggttggcgat gcatggtggc tggcacaagt gccggaagtg aactcggcac tggtgtcgat
    17041 caatccgcaa aatggtgccg ttatggcgct ggtcggtggc tttgatttca atcagagcaa
    17101 gtttaaccgc gccacccagg cactgcgtca ggtgggttcc aacatcaaac cgttcctcta
    17161 caccgcggcg atggataaag gtctgacgct ggcaagtatg ttgaacgatg tgccaatttc
    17221 tcgctgggac gcaggtgccg gttctgactg gcagccgaaa aactcaccac cgcagtatgc
    17281 cggtccaatt cgcttacgtc aggggctggg tcagtcgaaa aacgtggtga tggtacgcgc
    17341 aatgcgggcg atgggcgtcg actacgctgc agaatatctg caacgcttcg gcttcccggc
    17401 gcaaaacatt gtccacaccg aatcgctggc gctgggttca gcgtccttca ccccaatgca
    17461 ggtggcgcgc ggctacgcgg tcatggcgaa cggcggcttc ctggtggacc cgtggtttat
    17521 cagcaaaatt gaaaacgatc agggcggcgt gattttcgaa gcgaaaccga aagtagcctg
    17581 cccggaatgc gatattccgg tgatttacgg tgatacgcag aaatcgaacg tgctggaaaa
    17641 taacgatgtt gaagatgtcg ctatctcccg cgagcagcag aatgtttctg taccaatgcc
    17701 gcagctggag caggcaaatc aggcgttagt ggcgaagact ggcgcgcagg agtacgcacc
    17761 gcacgtcatc aacactccgc tggcattcct gattaagagt gctttgaaca ccaatatctt
    17821 tggtgagcca ggctggcagg gtaccggctg gcgtgcaggt cgtgatttgc agcgtcgcga
    17881 tatcggcggg aaaactggta ccactaacag ttcgaaagat gcgtggttct cgggttacgg
    17941 accgggcgtt gtgacctcgg tctggattgg ctttgatgat caccgtcgta atctcggtca
    18001 tacaacggct tccggagcga ttaaagatca gatttcaggt tacgaaggcg gtgccaagag
    18061 tgcccagcct gcatgggacg cttatatgaa agccgttctt gaaggtgtgc cggagcagcc
    18121 gctgacgccg ccgccgggta ttgtgacggt gaatatcgat cgcagcaccg ggcagttagc
    18181 taatggtggc aacagccgcg aagagtattt catcgaaggt acgcagccga cacaacaggc
    18241 ggtgcacgag gtgggaacga ccattatcga taatggcgag gcacaggaat tgttctgatt
    18301 aaaaaggcgc ttcggcgcct tttcagtttg ctgacaaagt gcacttgttt atgccggata
    18361 cgggtgaacg cgttatccgg ctaacaaaat cgtgaaaact caatatattg caggaacccc
    18421 ataagcctga taagcattgt gcatcaggca aacttcacgc atttacactc gcccctgccc
    18481 tttcaaccat tcgcgcacga ggaacagcgc gctgacatta cgcgcttcgt tgaagtcagg
    18541 gtcttccagc aaatccatca tatgcgccag cggccagcgc acctgtggta gcggctctgg
    18601 ctcatcgcct tccagcgatt ccgggtagag atcttgcgct accacgatat tcattttgct
    18661 ggaaaagtaa gacggtgcca tgctgagctt cttcaaaaaa gtcagatcgt tcgcaccaaa
    18721 tccaacctct tcttttagct cgcggttagc ggcttcgaag acgctttcac ccggatcaat
    18781 taatcctttc gaaaaaccta attcgtagga ttcagttccc actgcatatt cgcggatcag
    18841 gatcaggtga tcgtccacaa tcggcacaat catcactgct tcccggttgg ttggacgcat
    18901 tcgttcataa acacgccgca cgccattgct gaactctaga tccacgctct cgacggtaaa
    18961 cagtcgggaa cgggctacag tttcaacatt cagaatggtg ggtttttgta atgatttgct
    19021 catcgtggga tctatgctgt gaaatcagcg gttattgtgc gatatcggac acgctttcgg
    19081 caatgtgaat tgcatgttat ttacatttat gtaacttaat aaataatcgt cctcaaatca
    19141 aattaaaagt caataggttg aaataactcc aggaatttgc tgatattccg ccttcagagg
    19201 gtttgctatg atcagcggtt actgtgatgt gcttaatgat gctcaagtta aactccacgc
    19261 ttgccgatag ccaaccgcag aatcatgtat tgtgtccggt gcgactgacc acgcctgaca
    19321 gactaagtaa gatggggaaa gcatgagcac cattgtgatt tttttagctg ctttgctggc
    19381 ctgctcacta cttgcgggat ggctgataaa agtgcgatcc agacggcgtc agctgccctg
    19441 gaccaacgcc ttcgcggatg cgcaaacgcg taaactcaca cctgaagaac gtagcgccgt
    19501 tgaaaattat cttgagagcc tgacgcaggt attacaggtg cctggcccaa cgggagccag
    19561 cgcggcaccg atctctctgg cgctgaatgc cgaaagcaac aacgtcatga tgctgacaca
    19621 cgctatcacg cgttacggca tctctaccga cgatccgaat aaatggcgtt actacctcga
    19681 ttcggtagaa gtccacctgc cccctttctg ggagcagtac atcaacgatg agaataccgt
    19741 tgaactgatt cataccgatt cgctgccgct ggttatttca ctcaacggtc atacgctgca
    19801 ggagtacatg caggaaactc gcggctatgc cttgcaacct gttccgtcca cgcaggcgtc
    19861 gattcgcggg gaagagagtg agcaaatcga gctactcaat attcgcaaag aaacgcatga
    19921 agaatatgcg ctgagtcgtc cgcgcgggct gcgtgaagcg ttgctgatcg tcgcctcctt
    19981 cctgatgttc tttttctgcc tgattacccc ggatgtattt gttccgtggc tggcaggcgg
    20041 cgcgttactg ctgctgggcg caggtctgtg ggggctattc gcccccccgg caaaatcctc
    20101 cctgcgggaa attcattgtc tgcgcggtac accccgtcgt tggggattgt ttggcgaaaa
    20161 cgatcaggaa cagatcaaca atatttcact cggtattatc gacctggtct atcccgcaca
    20221 ctggcagcca tacattgctc aggatctcgg tcaacaaacc gatattgata tctatctcga
    20281 ccgccaccta gtgcgtcagg ggcgatatct ttcgctgcat gatgaagtaa aaaactttcc
    20341 gttacagcac tggctgcgca gtacgattat cgctgcgggc tcgctgctgg tgctgtttat
    20401 gctgttattc tggatcccgc tggatatgcc gctgaaattc actctctcat ggatgaaagg
    20461 tgcgcagacc attgaagcca ccagcgtaaa acaactggct gacgctggcg tgcgggtagg
    20521 cgatacattg cgtattagcg gtacgggaat gtgtaatatt cgcacttccg gaacctggag
    20581 cgcgaaaacc aattcacctt ttttaccgtt tgactgctcg cagatcatct ggaacgacgc
    20641 ccgctcatta ccattaccag aatctgaact ggtcaacaaa gcgacggcat tgactgaagc
    20701 agttaatcgc cagctgcacc ctaaaccgga agatgaatct cgcgtcagcg cctcattacg
    20761 ttcagcaatt caaaaatccg gcatggtatt gcttgatgat tttggcgaca ttgtactgaa
    20821 gacagcggat ctgtgttctg ccaaagatga ctgtgtgcga ctgaaaaatg cgctggtcaa
    20881 tctcggcaac agtaaagact gggacgcgct ggtaaaacgc gccaacgccg ggaagctcga
    20941 tggcgtgaat gtgttattac gcccggtgag tgcggaatcg ctggataacc tggtggcaac
    21001 ctccaccgcg ccgtttatca cgcatgaaac ggcgcgagcg gcacaatcac taaacagtcc
    21061 ggcccccggc ggattcctga ttgtcagcga cgaaggcagc gattttgttg atcagccctg
    21121 gccttcggca tcactttacg actacccgcc gcaagaacag tggaacgctt tccagaaact
    21181 ggcacaaatg ctgatgcata cgccgtttaa cgccgagggt atcgtcacaa aaatcttcac
    21241 tgacgccaat ggtacgcagc atataggcct tcatccgatc ccggatcgtt ccggcctgtg
    21301 gcgctatctc agcaccacgc tgctgctact gacgatgctg ggtagcgcca tttataatgg
    21361 cgtacaggcc tggcggcgtt accagcgtca tcgcactcgc atgatgaaga ttcaggccta
    21421 ttatgaaagc tgcctgaacc cgcaactgat caccccttca gaaagcctta tcgaataaca
    21481 cgtttgcgcg gcaggttatg ctaccctgtc gcgcaaattg cttcactctg gagattttcc
    21541 tcatgcatat caacattgcc tggcaggacg tagataccgt tctgctggat atggacggca
    21601 cgttgctcga cctcgccttc gataactatt tctggcaaaa gctggtgcct gaaacatggg
    21661 gcgcgaaaaa cggggttacg ccacaggaag cgatggaata tatgcgccag caatatcacg
    21721 acgtacagca tacgctaaac tggtactgtc ttgattactg gagtgagcaa ctgggtctgg
    21781 atatctgtgc gatgaccacc gagatgggac cgcgtgccgt actgcgtgaa gataccattc
    21841 cgtttcttga ggcactgaaa gccagcggta agcagcgaat tttgctcacc aatgcgcatc
    21901 cgcacaacct ggcggtaaaa cttgagcata ccggtctgga cgcacacctt gatttattac
    21961 tttccaccca cacatttggt tatccgaaag aggatcagcg gttatggcat gcggtggccg
    22021 aagctacggg tctgaaagct gaaagaacgc tgtttattga tgacagcgaa gcgattctcg
    22081 atgctgccgc gcaatttggt attcgttact gcctcggcgt gactaatcct gactcaggga
    22141 ttgccgagaa acagtatcaa cgccatccgt cactgaatga ctaccgccgc ctgatcccct
    22201 cgctaatgtg aaggagacgc catgaaagaa aaacctgctg ttgaggttcg actggataaa
    22261 tggctatggg ctgcccgttt ttataaaacc cgcgcgctgg cccgtgaaat gattgaaggc
    22321 ggtaaggtgc attacaacgg gcagcgcagc aagccgagca aaatcgtcga gctgaatgcc
    22381 acgctcactc tgcgccaggg aaatgacgaa cgcacggtga ttgtaaaggc gattactgaa
    22441 cagcgtcgcc ccgccagcga ggcagccttg ctgtatgaag agactgcgga aagtgtagag
    22501 aaacgcgaaa aaatggcgct ggcacgtaaa cttaatgcct taaccatgcc gcacccggac
    22561 cgacgcccgg acaaaaaaga gcgccgcgac ctgttacgat ttaaacacgg cgacagtgaa
    22621 taactgtcac ctgcaagaga gatgattatg ccgcaacatg accaattaca tcgctatctg
    22681 tttgaaaact ttgccgtgcg cggcgaactg gtaaccgttt cggaaaccct gcaacagatc
    22741 cttgagaatc acgattatcc gcagcccgtt aaaaacgtgc tggcagaact gctggttgcg
    22801 accagcctgt taaccgctac gctgaagttt gatggtgata tcaccgtaca gctgcaaggc
    22861 gacggtccga tgaatctggc ggttattaac ggtaacaaca accagcagat gcgcggtgtg
    22921 gcgcgcgtgc agggcgaaat tccagaaaat gccgacctga aaacgctggt cggcaatggt
    22981 tacgtggtga tcaccattac cccgagcgaa ggtgaacgct atcagggcgt ggtgggtctg
    23041 gaaggtgata ccctggcggc ctgcctggaa gattacttta tgcgttctga acagctgccg
    23101 acgcgcctgt ttattcgcac cggcgacgta gacggcaaac cggctgcagg cggtatgttg
    23161 ttgcaggtaa tgcctgcgca aaatgcccag caggacgact ttgaccacct ggcgacgcta
    23221 accgaaacca tcaaaaccga agaactgctg accttaccgg caaacgaagt gttgtggcgt
    23281 ttgtatcacg aagaagaggt gacggtttac gatccgcagg atgtggagtt caaatgcacc
    23341 tgctcgcgtg aacgttgcgc cgatgcgctg aaaacgctac ctgatgaaga agtcgatagc
    23401 atcctggcgg aagatggcga aattgacatg cattgtgatt actgcggtaa ccactatctg
    23461 ttcaatgcga tggatattgc tgaaatccgc aacaacgcgt ctccggcaga tccgcaagtt
    23521 cattaatgag tatgtccggc agagagagct ctgccggatt ttacgactaa tacacctgat
    23581 gcgctttgtc actcattcag attcacatcc agcctttcac catccactac aagatcgcgc
    23641 cacgctttag gcttcgttcc cagttttcca tccttcgcag ctgtgagtaa tttttctttc
    23701 gttatatcac gcggaagtaa actcatcgta agctcttgcc attctttctt gtcaggatca
    23761 aagccataga caatgtatct ttcatcatca aaggcaaaga ggatccgctc tgccctgcca
    23821 tcactattca gatcttgctc gaccagtacg caggcgtctt tttcaataca ggtcatcacg
    23881 ttatagcgat cctggattaa ggccgaccag aatgtcgcat ccggtttacc agaaccaggg
    23941 gcaattaaca cattttcggc taatactttt tccgatatct gttgctgaag atgttgctct
    24001 ccatctaacg ccatcagtag atcccgcgcg cgtttcgggt ctttcatata ctcggcatcg
    24061 cttttcagcg actcaagcgc cgcacgtcca tagcgaccgc tgtgttcgag catgtagata
    24121 gttacctggt ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacacta
    24181 atgcgcatac tgtccagcac cggcgaatta agaagcacca gtattaccaa caccaataac
    24241 gaaaccgcaa ggttcacttt gccctgaaga acaaggggat tttgcccttt acgccagacg
    24301 atactgacaa aataccccag cgaccatacc agtaacacca gcaccgccag cgcgccctgc
    24361 aagcggtcga cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgatg
    24421 aacacataca gcggagcaac cagcagagcg gttttaatca ggcaacgcaa cggccccgtc
    24481 cagggaagtg acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag
    24541 gccagcgtta acagcaaccc ggcggcggag atgtggcgag aaatcgcact caggcctgta
    24601 aacggcaggg tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg
    24661 gcgattaacg tgaacaactt ttgaatagag tcgattaaac gtgactgtgt tcgcgcgagg
    24721 atcaccgcca gcgcggtaac cagacctaac gtgagataaa taaaccagtc ggttgcaaaa
    24781 aagagcgttt tgaaaaacgt gatgccaaca agtttaaaca actcactcca cagtaaaagc
    24841 accagccacg tcagaccatt ggcgagaaaa atcaccagta atataagtac gttatgccat
    24901 actgactggt aaaaatagcg atagcggcta ctgtcattgc ggatgcgaag gctttgttgt
    24961 atccacggta gcaacaacat tgccatcagc agcagatagc aaccaaaatc ccaaagcgcc
    25021 ttttcagctc tccagggggt catgccatca gtttgccact tcagccagcc gctcatcccc
    25081 agcgtggcaa taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc
    25141 acggagaaga gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa
    25201 ctgtgatttt ttccggaaag ccaggcgatc agcaggtagc aaaccagacc ctgaagtaat
    25261 ccggtagcaa tcatccccca gcgtgttgcg ggtgaaagtt caacgttatc catgttggtt
    25321 atccagaatc aaaaggtggg ttaattatcg catccgggca gtagtatttt gcttttttca
    25381 gaaaataatc aaaaaaagtt agcgtggtga atcgatactt taccggttga atttgcatca
    25441 atttcattca ggaatgcgat tccactcaca atattcccgc catataaacc aagatttaac
    25501 cttttgagaa cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt
    25561 gacaggaatc acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag
    25621 ccttgtcgcg gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa
    25681 ggagcagtga aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta
    25741 tcagtgacgt acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc
    25801 tcgatccgag cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg
    25861 tcgataccgg gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata
    25921 ccactcgcga tactttctgg tgggcagaca aaggcaaagg taagaacgat aacaaacctc
    25981 tctctccgga aacctggcaa catctgaaag gcctggtgac caaacagctt tccggcaaac
    26041 gtctgttcgt tgtcgacgcc ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt
    26101 tcatcaccga agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg
    26161 atgaagagtt ggcaggtttc aaaccagact ttatcgttat gaacggcgcg aagtgcacta
    26221 acccgcagtg gaaagaacag ggtctgaatt ccgaaaactt cgtggctttt aacctgaccg
    26281 agcgcatgca gctgattggc ggcacctggt atggcggcga aatgaagaaa gggatgttct
    26341 cgatgatgaa ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg
    26401 ttagcgagaa aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc
    26461 tttccaccga cccgaaacgt cgcctgattg gcgatgacga acacggctgg gacgatgacg
    26521 gcgtgtttaa cttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg
    26581 aacctgaaat ctacaacgct atccgtcgcg atgcgctgct ggaaaacgtc accgtgcgtg
    26641 aagatggcac tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc
    26701 cgatctatca catcgataac atcgttaagc cggtttcaaa agcgggtcac gcaacgaaag
    26761 ttatcttcct gaccgcagac gcctttggtg tgctgccacc ggtttctcgt ctgactgccg
    26821 atcaaaccca gtaccacttc ctctctggct tcaccgccaa actggccggt actgagcgtg
    26881 gcatcaccga accgacgcca accttctccg cttgcttcgg cgcggcattc ctgtcgctgc
    26941 acccgactca gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt
    27001 atctggttaa cactggctgg aacggcactg gcaaacgtat ctcgattaaa gatacccgcg
    27061 ccattatcga cgccatcctc aacggttcgc tggataatgc agaaaccttc actctgccga
    27121 tgtttaacct ggcgatccca accgaactgc cgggcgtaga cacgaagatt ctcgatccgc
    27181 gtaacaccta cgcttccccg gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt
    27241 ttatcgacaa ctttgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg
    27301 gtccgaaact gtaatgattt gaagctggag aatatctatc cagtatctta tagaaagcaa
    27361 aacgggaggc accttcgcct cccgtttatt tacccttctt ttgtcgtgct ctgcgcccgc
    27421 gttaccggca caggcagcca ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc
    27481 tccagcatcc cgttatggtt atccacgata cgctgcacaa tcgccagccc taatcccgtg
    27541 ccgctaatgg tgcgtgcact gtcgccgcga acaaacggct ggaaaaggtg cttacgttgt
    27601 tccggcgcaa ttcccggacc gtcatcttcc acctggaacc aggcgcgatt cggctccgtc
    27661 ccgctgctga ctttgatcca gccattgccg taacgggcgg cgttgaccac catattcgcc
    27721 accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg
    27781 gtttcaattt cccgctcata gccactttcg gcagcaataa cctcaccgag tactgcgttg
    27841 agatccgcca tttccatcgg catctcctgt ccggtgcgca ggtagtcgat aaactgctca
    27901 atgatggcat tgcactcctc gatatcttta ttgatcgatt ctgccaggta gccatcctgc
    27961 tcgctcatca tctcggtcgc caggcgaata cgcgtcagcg gtgtacgcaa gtcgtgactt
    28021 acccccgcca tcagcagcgt gcggtcatcc gccagttgct taacaccagc cgccatatgg
    28081 ttaaaggcgc gggtaacgga acgcacctcg gacgcgccat actcacgcag cggcggcgga
    28141 ataatccctt taccaacctg caaggctgcg tgttcgagat cgaccaacgg tcgattctgg
    28201 atacgaataa acagccacgc cccgcctatc gccaatagca taatcgccaa cgtatagcgg
    28261 aacagcggag agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg
    28321 ggcgacagcc aggttttcag ccagacgaca ggcgaacttt tgttgacctc aacgcgcact
    28381 tccgtcgggc cgcccagttg ctgtgccatc tgatggctta agaattcata gtgttgcgcc
    28441 caacgcagac ctgcctcttc ggcagcctcg ttggagtaga gagagatccc cagctcacgg
    28501 tagatctccc gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt
    28561 ttgtcggtca tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc
    28621 ggcaaaatcg cgaagttcag caccaccaga taagtcgtca ccaggctggc gaacagcaag
    28681 gtgacgatga gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc
    28741 atgctttaga gccgtccggt acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac
    28801 gcggatgcgc tggatcttct tccaccatgc ggcgcagacg agaaatctgc acgtcgatgg
    28861 agcgttccat tgcggaatat tcacgaccac gggcaaggtt catcagctta tcgcgggaga
    28921 gcggctcacg cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg
    28981 gcatcggctc gtcttcgcgg aacatttcgc gcgtaccgag gttaagtttg aacttaccga
    29041 aagcaattac cgcctcttcc tgtgacggtg cgcctggtag ctcgttcgcc tgacgacgca
    29101 gcaccgcacg gatacgggcc agcagttcac gcgggttaaa cggttttgga atgtaatcgt
    29161 cagcgccaat ctccaggcca acgatacggt ccacttcttc ccctttcgcc gtcaccataa
    29221 tgatcggcat cgggttgctc tgactacgaa gacgtcggca aatcgacaag ccatcttcac
    29281 caggtaacat taaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca
    29341 tctgttctgc attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca
    29401 gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat cttgtagttc tcttgcattg
    29461 tttgtactcc caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt
    29521 cgtcaccagc taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag
    29581 cagcttaagt atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt
    29641 atacccaatg tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg
    29701 gttacccggg aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa
    29761 cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat
    29821 gctgactatc agtataataa aaagcgcctg cgtgaaatcg accgtcgcgt gcgctatctc
    29881 actaaatgca tggaaaatct caaaatcgtc gattactccc cgcagcagga aggcaaagtc
    29941 ttttttggcg cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt
    30001 gtcggctacg atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc
    30061 cgcgcattgc tgaaaaaaga agtcggcgat ctggctgtgg tgaatactcc tgccggggaa
    30121 gcgagctggt atgttaatgc tatcgagtac gtgaaaccgt aaggaagagt cttaacctcc
    30181 tgccgatggc tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg
    30241 aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt
    30301 caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg
    30361 ccttttattg cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt
    30421 aatctggaaa cgcgtctggg atacctgcgc gagctggaag agagacgtca ggcgatcctc
    30481 aagtccattt ccgagcaagg caaactcacc gatgatctgg cgaatgccat caacgccacc
    30541 ctaagcaaaa ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc
    30601 gggcaaatcg ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg
    30661 tcacacacgc cagaagtcgc cgctgcgcaa tatattgatg ccgataaagg cgtggcagat
    30721 accaaagccg cgctggacgg cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc
    30781 gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ttctacggtg
    30841 gtgagcggta aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg
    30901 ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta
    30961 cttcagcttt cgctgaatgc cgatccacag ttcgatgagc cgcccaaaga gagctattgc
    31021 gagcaaatca tcacggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg
    31081 cgcaaaggcg tggtgagctg gacgtggcgc atcaaggtgc tgatgcatct ggaaaccgaa
    31141 ctgatgggca ccgtgcgcga acgtgcggaa aatgaagcaa tcaacgtctt tgcccgtaac
    31201 ctgcacgatc tgctgatggc tgcccctgcc ggactgcgtg caacgatggg cctcgatccg
    31261 ggtctgcgta ccggggtaaa agtggcggtg gtcgatgcca ctggcaaact ggtagcgacc
    31321 gataccattt acccgcacac cggacaagcc gcaaaagcag cgatgactgt tgctgcgctg
    31381 tgtgaaaagc ataacgttga actggtggcg atcggcaacg gtacagcttc ccgcgaaacc
    31441 gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc
    31501 gtcagcgaag ctggcgcgtc ggtttactcg gcttccgagc tggcagcgca ggagttcccg
    31561 gatctcgacg tttcgctgcg tggcgcggtt tctatcgccc gccgtttgca ggatccgctg
    31621 gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc
    31681 agccagacgc aactggcccg caaactggat gcagtagtag aagactgcgt aaacgccgtt
    31741 ggcgtcgatc tcaacaccgc ttctgttccg ctattaactc gcgtggcggg cctgacgcga
    31801 atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gccagttcca gaaccgtcag
    31861 caactgctga aagtcagccg tctggggccg aaagccttcg agcagtgcgc gggcttcctg
    31921 cgcattaacc acggtgacaa cccgctggac gcctctaccg ttcacccgga agcctatccg
    31981 gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc
    32041 agcgaactgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgaca
    32101 gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa
    32161 accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc
    32221 gaaggcgctg tcaccaacgt caccaacttt ggcgcgtttg tcgatatcgg cgtgcatcag
    32281 gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg
    32341 gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt
    32401 atcgccctga ctatgcgtct ggacgagcag cctggcgaaa ccaactctcg tcgcggcggc
    32461 ggtaacgatc gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag
    32521 cctgccggta acagcgcgat gatggacgcg ctggcggcag caatgggcaa aaaacgttaa
    32581 acgcccggac tggcccctaa acctgcgcga taaggcgttc acgccgcatc tggcaacgaa
    32641 cgccttgcct gacataaaag tgccggagaa tatctccggc atatttattc cacagccaaa
    32701 ctcacaatat atttcgttaa tatttatcac ttcattaaca actgaaacct taattaaaca
    32761 ttagccagtc ctggtaattc gctattcgaa ttatattttc gctgcgatat aaccttgagc
    32821 cacatcaaca ttgagtcaga ttattattca aaccaacatt cgcacacatt ttaagtattg
    32881 ctgatagaaa ccattctcat tatcattgta ctattgatta tttaatctct ccttcgttgg
    32941 caaatcatct ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga
    33001 gaaacaagta ggcacctatg caatacactc cagatactgc gtggaaaatc actggctttt
    33061 cccgtgaaat cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct
    33121 cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc
    33181 gtgtgagcct ggtattacgc aaaaaagatc tggccttatt agaagtggaa gcggtttcct
    33241 gttaatacgg tgataacaac aatgaaaaaa ttaaccatag gcttaattgg taatccaaat
    33301 tctggcaaga caaccttatt taaccagctc actggcgcac gtcagcgtgt aggtaactgg
    33361 gctggcgtta ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg
    33421 ctggtggacc tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat
    33481 gagcaaatcg cctgtcacta cattttgagt ggcgacgccg acctgctgat taacgtggtg
    33541 gatgcgtcta accttgagcg taacctgtac ctgacgctac aactgctgga actcggcatt
    33601 ccctgcatcg tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa
    33661 attgatgctc tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc aacccgtggt
    33721 cgcggtattg aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa
    33781 ctggtgcatt acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct
    33841 tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc
    33901 tacagccgcg cctacgccgg tgaagcgtcg cagcatcttg atgccgccct cgcccgtctg
    33961 cgtaatgaga tggacgatcc ggcgctgcac attgccgatg cgcgttacca gtgcattgct
    34021 gccatctgtg atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg
    34081 gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac
    34141 ctgatgttcc tgctggctat caacatcggc ggggcgttac agccgctgtt tgacgtcggc
    34201 tccgtggctc tatttgtgca tggtattcaa tggattggct acacactcca cttcccggac
    34261 tggctgacta tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg
    34321 gtgccgcaga tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg
    34381 gcgcgtgcgg cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc
    34441 tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg tgcacgtacg
    34501 cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg caccgtttat gtcctgcggc
    34561 gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgcgctg
    34621 gcggtcttct cgctgtatat gctcggtatc gtgatggcgg tgctgactgg cctgatgctc
    34681 aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat
    34741 gtaccacacg ttaaaagcct gattatccag acctggcagc gtctgaaagg cttcgttctg
    34801 cgtgctggta aagtgattat catcgtcagc attttcctga gcgctttcaa cagcttctcg
    34861 ttgagcggga aaatcgtcga taacatcaac gactcggcgc tggcttccgt cagccgggtg
    34921 atcaccccgg tcttcaagcc gattggcgtg catgaagata actggcaggc aacagttggc
    34981 ctgtttacgg gtgcaatggc gaaagaagtg gtggtgggta cgctcaacac cctctacacc
    35041 gcagaaaata ttcaggacga agagttcaat ccggcggaat ttaacctcgg tgaagagttg
    35101 ttcagcgcgg tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg
    35161 atgaacccca ttgaagctag caaaggcgac ggcgaaatgg gtaccggggc gatgggcgtg
    35221 atggatcaga aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg
    35281 tatgtaccat gtatctcggt gatgggagcc atcgcccgtg aatcaagccg tggctggatg
    35341 ggcttctcca tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcaa
    35401 gtcgccagct acagtcagca cccaacttac agcctggtgt gcattctggc ggttatcctg
    35461 tttaacatcg tggttatcgg tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg
    35521 ctggcaaccc gcaagtcggt aagtagttgc tgcgcagcca gcaccaccgg tgattgccat
    35581 taatggcttc acttattcag gtgcgtgatt tgctggcgtt acggggccgt atggaagcgg
    35641 cccagataag ccagacattg aacactccac agccaatgat taacgccatg ctgcaacaac
    35701 tggaaagtat gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctccggca
    35761 gttgtaaaag ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac
    35821 catactccat cgcctgtttt gaaaagcccg gtatgcgttt gcatccgggc ttttttgcgt
    35881 gcggctttcc ataaaaatgc aattcttgca gcgcgacgta agttcctctg aaagcgtctc
    35941 gcagggatga aaactggcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca
    36001 aacaacaggt gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg
    36061 ttcaaactct ttttacgcca accggagacg gcacgcgatt ttcttgcgtt tcatttacca
    36121 gcacccattc acgcgctctg tgatatgaaa accctcaagc tggagtcgag cagctttatt
    36181 gatgacgatc tgcgtgaaag ctatcccgat gtgctgtggt cggtgaaaac agaacaagga
    36241 ccaggataca tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt
    36301 cgcatgatgc gttacgctat tgccgcaatg caaaatcacc ttgatgccgg atacaaaacg
    36361 ttgccgatgg tggtgccatt gttgttttac cacggtattg aaagccccta tccctattcg
    36421 ctgtgttggc tggattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca
    36481 tttccgctga ttgatatcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgcatg
    36541 acgctgctgg agttaattca aaaacatatt cgtcaacgcg acctgatggg gctggtagag
    36601 caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa agggctgttt
    36661 aattacatac tgcaaaccgg cgatgcggta cgttttaacg attttatcga cggcgttgcc
    36721 gaacgatcac cgaaacacaa ggagagttta atgactattg cggaaagatt gcggcaggag
    36781 ggggaacaat ccaaagccct gcatatagcc aaaataatgc ttgaatccgg agtccctctt
    36841 gcagacatca tgcgctttac cgggctgtca gaagaagagt tggctgcggc gagtcagtaa
    36901 agttctgtct cgccattcaa aagtgcctac accctctgct tcaacgccac cagcacgtga
    36961 cgaaactcga ccggatgcga aataaacggc gcatgagccg ctttggcgaa gatgtatgac
    37021 tcgctgtgtg gccagagttt atccagcatc ggcaccactt tgcgcggcac cagaccatcg
    37081 agatagccat acagtcgcaa aaacggcatg ggtacgtttt gcagcggcaa gcggagatca
    37141 accgttttga ggatttccag cccaccattg agcacgtcaa cctccggcat cggtaacgcc
    37201 agaacggttt tcttcaacgc ccgcgcatcc tgacgcgccg tttcagtacc catggtttgt
    37261 aacgcaagga accgttccac cgtacgctga aaatcatcac tgagttgctg ctgaaatccc
    37321 gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca aggtgacgac
    37381 gccacggtga ccagcgcctg aacacgctcg ggatgggtta acgcaatctg gcttgccacc
    37441 agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgtcg cagcacggct
    37501 tcggccatct ccgcaagtga cagcgcacca aatccccggc tacgcccgaa gccgggcagg
    37561 tcaacaagat gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg ccacacttca
    37621 gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc tttggtctgc
    37681 caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga acatatgcta
    37741 acagtaccgg gattatgctg gctatgccga atgccactgg cattaggtca ttgggggatt
    37801 tgttcggtct gctcacgcgc cgcccgtaca gataaaacgt tatgcccaca atgtggatta
    37861 cccgccacac actcccatct tccctgcggt cgctgcctgc aaaaaccgcc gccctggcaa
    37921 agactggtca cggttgccga ctatgcgccg ccgttaagtc tgctcatcca ccagcttaag
    37981 ttttcccggc gcagcgaaat cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta
    38041 cacgctcgtc gcaccaccgg gttgcaattg ccggatcgta tcatcagcgt tccgttatgg
    38101 cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca gccgttatca
    38161 cgctggttgc actgccaatg ggatagcgaa gccatcacac gtacacgggc cactgcgacc
    38221 cagcattttc ttagcgcccg gctacgcagg cgcaacctga aaaatgcctt tcgccttgaa
    38281 ttgcctgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac cggaagtacc
    38341 gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc
    38401 ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta aaatagtcaa
    38461 ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact ttgccaaact
    38521 gctggcaaat caggaagaag ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc
    38581 taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca ccgacaccgc
    38641 cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat acctggaaga
    38701 tgcagagatc gattttgtta ccgaccagtt gggttctcag ctaacactga aagcgcctaa
    38761 cgccaaaatg cgtaaagtgg cagacgatgc accactgatg gagcgcgtgg agtatatgct
    38821 gcagtcgcag atcaacccac agcttgctgg tcacggtggc cgcgtttcac tgatggaaat
    38881 caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt gttccatggt
    38941 cgatgtgacg ctgaaagaag ggatcgagaa gcagctacta aacgaattcc cggagctgaa
    39001 aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact aagttatccc
    39061 ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac gttgaacatg
    39121 tgccagacgt tggcgcagca ttgctttggc aaatccctcc cttcgtttcc ccgcgataat
    39181 atgaccaacc tctcataatt taaatttacc ccgctctggt gattctcaaa cgccagatgt
    39241 tacccgtatc attcacgtgg gtaccaaaca tactcctgac atctgactac aataattagt
    39301 tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga taattagagc
    39361 ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa tcatgccatt
    39421 agtcattgtt gctatcggtg taatcttgtt attactcctg atgatccgct tcaaaatgaa
    39481 cggcttcatc gctctcgtcc tcgtggcgct tgcggttgga ttaatgcaag ggatgccgct
    39541 ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta gccttgccct
    39601 gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg gcgcacaacg
    39661 tatcgccacc acgctgattg ccaaatttgg taaaaaacac atccagtggg cggtggtact
    39721 gaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggctttgtgc tgatgctgcc
    39781 gctggtgttt accatcgcgg cttctgcgaa tattccactg ctgtatgttg gtgtaccaat
    39841 ggcagctgca ctgtctgtga cccacggctt cctgccaccg catccgggtc cgactgcgat
    39901 tgccaccatt ttcaatgccg atatgggtaa aaccctgctg tacggtacta ttctggcaat
    39961 cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc
    40021 aattccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg
    40081 cgtcagcgtc tggacctctc tggtgccggt agtactgatg gcgatgcgtg cgattgccga
    40141 aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg gtgacccggt
    40201 aatggctacg ctgattgccg tgctgattgc gatgttcacc tttggtctga accgtggtcg
    40261 ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg cgatgatgct
    40321 gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa
    40381 atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg cctggtcgat
    40441 tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg
    40501 tatcgcggca ccgctgattg caacgacggg tgttagcccg gagctgatgg ttattgcggt
    40561 gggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc tgttcaaaga
    40621 gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg aaaccattat
    40681 ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat aacactgact
    40741 gccggatgca tcgcatccgg catcaaccgc actctacttc ttcttcgctg cagctctgcg
    40801 ccgtctgtcc aaatccttca gcaacttgtt cacgccatca tcggcaaaca tcgtctcaag
    40861 cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag gaatattcac
    40921 cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac tgttactgtc
    40981 ggcaatgtag cgctgcaaac cacgattaag cgtcggcgtc atcgacatca acgatgcctt
    41041 atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca gcccttgctt
    41101 cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat acagccccag
    41161 ggttttgccc agcgttagat ccccgctctc ccagtagccg cgtagcgtag gcaggtcatg
    41221 tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga acgtcttctc
    41281 gtggtcgttt tcgaaataga gcactttgta ggagtacaca ccgctgctgc gcagcttacc
    41341 gacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc gatgacgttt
    41401 actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat acgcgccctg
    41461 atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca catggtcaat
    41521 tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg gttcataagc
    41581 acgcgcggtg atgatatgcg ggtccattgg cggcaatccc cagttctgcc ccaacgggcc
    41641 gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa tacagttcac ggtcgcacca
    41701 ggtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc caatcggcat
    41761 ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcct gccactgcaa
    41821 ccagagataa aaatcaacgt catcgcgatg ttcttcgcag aactgacgca cttctggtga
    41881 atccacgttc tgatacatct ctggccatgc aggccagccc cagcgcattt cgtcctcttt
    41941 cacttgctgg gcatgtagcg catcaaaggc tgcctgccag aacaggctgt cgccttgatc
    42001 tgcaacaaac tggcgaaacg cggccatctg ctcatcatca cgttgcgcga aacctttcca
    42061 cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcga cccagtcggc
    42121 atcccgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc
    42181 ttcgctaaga tggaaatctt cgacggcgtt aacgtcgata taaatcacat tcagccagcg
    42241 gcgagaagac gggctatacg ggctggcgct ctccggattt gccggataga gcgcatgaat
    42301 cgggttcaga ccaatgaacg atccgccacg ttttgccaca tccactagca tcgctttgag
    42361 atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa gctggacgca
    42421 ggcaccccac agcttttgtt tattcagcaa cgcctgcggt tcgtaacagc gtttcggggc
    42481 gacaatcacc ctgcaatgcc cgcgctggtc ttcctgggta agcgtcagcg tgtgataacc
    42541 ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttatatggc ctttgtactg
    42601 cgttccttct tcggtggtca gcagccagct atattcgccg ctgccctcca ccaccatcgg
    42661 catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg ccactttcgt
    42721 ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg
    42781 cggtttaccg tgggcattga tgtaatgagg gctaatcccc gccgccagcg cggcattatc
    42841 cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac gagcctgata
    42901 atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga ggatcgccgc
    42961 gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg cctctacata
    43021 ggctgcgaaa tccgccatca ccagatacgg atcgccgccc tgtttgccga tactgtgcag
    43081 catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca gctctttcaa
    43141 tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc ctttggccag
    43201 aattgccttc acttgttcca cggtatgacc aaaaataaag atattttctt caccgacttt
    43261 ctcggcgatt tcaacgttcg ccccatccag cgtaccgaca gtaagcgcac cattgagcgc
    43321 cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt gttcggagat
    43381 atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatccggca ggaacaccac
    43441 cttcaactta tcgccaacca gcggatcgtt gttgatcacg tcagccactt tgttgatcgc
    43501 aaagataata tttttcgcca ggtagtagcc cggtgccgct ttcgcgccga agaggaagac
    43561 gcgcggtacg cgatcagcct gtgggttttc acgaatttct ttgtacaacg ccagaatatg
    43621 cagcagattc aggtgctggc gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat
    43681 cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca gacggacttt
    43741 attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag cgaatttttc
    43801 cagattgatc agctgatcga gatcgttagc ccactctttt ttcagtgatt tatccaacag
    43861 agccgccagt gccgggttgc actgtttgat ccagcgacgt ggggtaatac cgttggtgac
    43921 gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt tcaccaccag
    43981 atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac acaggttcgc
    44041 catatgcact tgtttgtcgt gcaccaccgc cagtttagcc cacacttttt catcgcccgg
    44101 ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa tctgcatgtg
    44161 gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt ctggcatcag
    44221 ggtatggttg gtgtaagcga aagttttgct ggtaatggcc caggcgtcat cccagctcat
    44281 ctggtgctca tcgatcagca cgcgcaacag ttctggaatc gcgatagttg ggtgggtatc
    44341 gttcagctga ataacttcgt aatccgccag ttcgtgcagt ttacgccccg ccagatgatg
    44401 gcgacgcaaa atatccgcta ccgaacaggc acactggaag tactgctgca tcaagcgcag
    44461 ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt tttccgcatt
    44521 gatgccctgc tgttcggcac gcaggaaatc accgtcgtta aatttagtca gatcaaacgg
    44581 atgcgcgtgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac gatagccgac
    44641 aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt
    44701 cgtcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc ggaaccacgg
    44761 gtagttactg cgatgccagt catccggcgc ttcaacctgt ttgccatcca caaaagactg
    44821 gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac cgacagttgc
    44881 cattgaatcg aggaagcacg ccgccagacg tcccagacca ccgttaccca gcgcagggtc
    44941 gatctcttct tccagcaggt cagtcagatt gatgtcataa gccttcaacg aatcctgtac
    45001 atcctgatac cagccgagat tcaacaggtt gttgcccgtc agacgaccaa tcaaaaactc
    45061 cattgagatg tagttaacat gtcgctgatt cgccaccggc ttggcgaatg gctgagcacg
    45121 cagcatttcg gccagtgctt cactcactgc cagccaccac tggcgaggag tcatttcagc
    45181 cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa attgcttatc
    45241 gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac acaacgttat
    45301 cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta ggcagggagg
    45361 agttgcgggg atgagcaagg aaatgtgatc tcgaccactt aaagctagtg caaaccacag
    45421 gattagcagc aaatcaatgc catacagcgc agaaaatctg tatctaagtg caaaaaatgg
    45481 ccgttgcgta ttttcaaaaa gcgaaaggta actctataaa ttaagtaaag gagtgaaaca
    45541 gtttcacaag taaaatatcc agtgtgctcc atctcattct taatagattt attaagatca
    45601 tctttttaga tagcactttc atcaggaatg aagaagaaac ccttgcttaa atgaatctga
    45661 tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat
    45721 aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc
    45781 attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata
    45841 tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ttaagtcgtc
    45901 cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga
    45961 acaacttccg gctggcgctg atcacaagtc ctgcgggcta cggtaagacc acgctcattt
    46021 cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggacgaa ggtgataacc
    46081 agcaagagcg tttcgccagc tatctcattg ccgccgtgca acaggcaacc aacggtcact
    46141 gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg
    46201 cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact
    46261 atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac
    46321 cagaaaatct caccctggtg gtgttgtcac gtaaccttcc gcaactgggc attgccaatc
    46381 tgcgtgttcg tgatcaactg ctggaaattg gcagtcagca actggcattt acccatcagg
    46441 aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca gaaagcagtc
    46501 ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc ctctccgccc
    46561 ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc aatgccagcc
    46621 atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca acgcgccatt
    46681 ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc actcatcacc cgtgtgaccg
    46741 gcgaagaaaa cgggcaaatg cgcctcgaag agattgagcg tcaggggctg tttttacagc
    46801 ggatggatga taccggcgag tggttctgct atcacccgct gtttggtaac ttcctgcgcc
    46861 agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa
    46921 gctggatggc ccagggattc cccagcgaag cgattcatca tgcgctggcg gcaggcgatg
    46981 cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac catagcgaac
    47041 tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc
    47101 agttggtgtt attgcaggcg tggctgatgc aaagccaaca tcgctacggc gaagttaaca
    47161 ccctgctggc ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcacgcag
    47221 aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatccggat gaagcggaac
    47281 ggctggcaaa actggcactg gaagagctac cgccgggctg gttctatagc cgcattgtgg
    47341 caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc
    47401 taatgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac gccttatgga
    47461 gtttaattca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa
    47521 cgcaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagttg ccgatgcatg
    47581 agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg
    47641 aagcgtcggc gcgtagcggg attgaagtct tatcgtctta tcagccacag caacagcttc
    47701 agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta
    47761 gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct
    47821 ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg
    47881 ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc
    47941 aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg
    48001 tcctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta
    48061 acctgttgct gctaaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg
    48121 tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg
    48181 aaggcgaagc gatggcgcaa caactgcgcc agctgattca actcaatacg ctgccggaac
    48241 tggaacagca tcgcgcacag cgtattctac gagaaatcaa tcaacatcat cggcataaat
    48301 tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac
    48361 tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg atttactccg
    48421 gttacagcaa cgaacaaata gcgggtgaac tggaagtggc ggcaaccacc atcaagacgc
    48481 atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg
    48541 cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttgtatagcg tttttatgta
    48601 cactctttat actgtatata aaatcgcact acaggctcat gaatatgaaa ccggaaagca
    48661 aagaagcgcc aatcaatatc agagcgaaag cctcacagcg tgacctgatt gatatggcag
    48721 cgaatctggt cgcaaaatcg cgcactgatt ttatgctgga tgccgcatgc cgtgaagcgc
    48781 aagacatctt attagatcaa cgactattca tccttgatga cgagcaatac gatgcgtttc
    48841 tcgctgccct ggacgccccc atcactgctg agcgtcaggc caaaattaac gcgttgatga
    48901 atcgtaaatc gccatgggaa taacggctcc tactccccta acgtctgaac ataatctggc
    48961 tgacttttgc tgttccgatc acgggatgaa tgaatggcta aaaaagaaag cgttaaaaaa
    49021 tcacagctcc ggtctgtctc gtgtttacgt tatctgtatt gccaataccc gccaggtaat
    49081 cggctattac tgcctttcta caggcagtat tcaacgtaat ttggcccctg gagccatgcg
    49141 acgtaatgct cccgaatcgt tgcctgttgt cgtattaggc agactggcaa ttgatcaagc
    49201 ctgggcaggt aaagggttgg gcgtcgcatt gttgaaagat gcagtctatc gcacaatgtc
    49261 catagctcaa caggtagggg ttcgtgcgct cattgttcat gccttagacg attcagtacg
    49321 caatttctat ctgaagtatg cctttgtacc ttcaccattc cagtcactta ccttactgta
    49381 tcccattact ctggagtaat ttacctctac cggatagtca cactgatgag tctgatccgg
    49441 gagggatttc aatcagtgag tttggttatc atcacccgcg ttacgccatc tgtttcagcc
    49501 agagtaaacc gcaccggcaa gaaacgctcc accacggcga tattggtcag cagatggcat
    49561 gagggatggg cgaccgtaaa ttgtcccgcg cccgccagcg ccattggcaa cactagctgg
    49621 tcagcaagat attcccccac tgccgccggg cttgccaggt agcgtttcac ctctttcacc
    49681 aactgtgccg caaccacctc ggcactgacg cgcttttcac cgacgacaaa aaagcgttcg
    49741 gtgatatttt cactttcgac ttcaagcgag acggtattac ccggcccctg gtcgcgcggc
    49801 aggttatgaa tattctgctc atgcagggaa aaacttgccg ccagcgtagc gatttcacgc
    49861 tcagcgacat gccgcggcac gccagctaat aacacctctc cacgcatctg cacaatgttc
    49921 ccgcgctcgc caagttgcaa ggtgttaaac gatgccaccg gcgagacttc cgttgccacc
    49981 acgccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg atgaattcct
    50041 attttcgcca gcagcggctc caacacccgg cggataaaat ccgcaggcgg ggccgacggg
    50101 ttatcggtac cgccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc
    50161 agcaccgttt gcagcaccag cgtacaactt cctgcgctac cgatagcaaa gcgataatcg
    50221 ccgccacgca cggtgccggg ccggaagacc agacgctgcg accccagctc cgcgccttcc
    50281 accgtcgccc cgcaaatttc cgtcgccgcc tttaccgccg tcagatgctg gcgcaacagt
    50341 cccggttttg cccgcccggc acgaatgccg gtgatggtaa atggctggcc ggttatcatc
    50401 gacagactca gcgccgagcg catgatctgc ccgccgcctt cgccctgtgc gccatccagc
    50461 gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgtag ggtgtagata
    50521 acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata cgccatcggg
    50581 atttcgtcga tcacttcggc atctttacgg cattccacat gcacggtggc acgaatttga
    50641 tcttccacgc tgaacatttt tttcgcttta gtacggctca taacccgccc ggcaccgtgg
    50701 ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct tttcgctccc
    50761 atcgaccctg gaataattcc atattgacca gcacgcgcag acaccgcgcc tttacgcgtc
    50821 acgtagatct cttcaccaaa gtgctgttct ttttgcacat agttgtggtg acagttgatc
    50881 tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct ctgcaacgcc
    50941 gttactacgt tttccatcat cacatcgcgg ttaaggctgg caaaaagctg cgcccaggcc
    51001 acggctttca ggtaatcatc aaagtattcc gtaccttcca taaagtacgc cagatcacgc
    51061 gacggcaatg tttcaagcgt ttcctgcatc tctttttgcg ccagatcgat aaagtaagtc
    51121 ccgatggcat taccaattcc gcgtgaaccg gagtgcagca taatccacac ctggtccgac
    51181 tcatcaaggc agatttcaat aaagtggtta cccgttccca gcgttcccag gtgtttatag
    51241 ttgttagtgt taaggaaacg cggatatttt tgcgttaacc actgataacc ggcctccagc
    51301 tcagcccatt tcgcatcgac gttaacaggc gggttttccc atgcgccttt atcacgttta
    51361 caacggccag tggtacgccc gtgcggcacc gcagtttcaa tcgcctgacg cagctccgcc
    51421 aggttttccg gcaggtcagc cgccgttaaa gcggtacgta gtgcgttcat tccacagcca
    51481 atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac gctaccaatg
    51541 gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa aataaacggc
    51601 atcttcgccg tattaataag ttgctgacgc gcatcggctt ctaccggcac gcctttggtc
    51661 cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt gtttttctct
    51721 tttcgttgtt tgcggtcctg ataattgcaa ccgtcgtgcc agaaaattaa ataagcagat
    51781 taatttttta attcattgtt ttaaaaagat tatatcttta cgtccgtaaa cggagatttc
    51841 ccgcaaagcc aatttaccga taatgaaata tcgtctttta taaggatatc taagatgcgt
    51901 aaaacagtgg cttttggctt tgtcggaaca gtgctggatt atgccgggcg cggcagtcag
    51961 cgctggtcga aatggcgtcc gtcactctgt atatgccagc aagaatcgtt agtcatcgat
    52021 cgactggaat tattgcatga cgcccgctcg cgctcgctat ttgaaacgct taaacgcgat
    52081 atcgccagcg tttcgccaga aaccgaagtg gtgagcgttg agattgaact gcataacccg
    52141 tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta cgagtttcag
    52201 ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc gcagatttgc
    52261 tggtttctgc tggctgaagc gcgttacctg cccgcccggt tgatacaatc ttcacctccg
    52321 cgcaaaaaag aacagccgcg cggcgcaggt gaagtgacga ttatcgatct cgatttaagc
    52381 ctttataacg ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct tgattttctt
    52441 aagtccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca gattgaaaaa
    52501 gtagcgatta aatcccgcgc accgatcctg ctaaacggcc caaccggtgc gggcaagtca
    52561 tttctggcgc gacgtatatt tgaattaaaa caggcgcggc atcagtttag cggcgcgttt
    52621 gtggaagtga actgcgcaac cctgcgcggc gataccgcca tgtcgacgct gtttggtcat
    52681 gtgaaaggcg cgtttaccgg ggcacgggaa tctcgtgaag gattattacg cagcgccaac
    52741 ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca ggcaatgctg
    52801 ctgaaagcca ttgaagaaaa aaccttttat ccgtttggca gcgatcgcca ggtgagcagt
    52861 gattttcagc ttatcgccgg aacggtgcgt gatttgcgcc agctggttgc cgatggcaaa
    52921 ttccgcgaag atctatacgc gcggatcaat ctctggacct tcaccctgcc ggggctacgc
    52981 cagcgtcagg aagatattga acctaacctg gattatgaag tggagcgcca cgcttcactc
    53041 accggcgaca gcgtgcgttt taacaccgaa gcgcggcgcg cctggctggc ctttgcgaca
    53101 tctccccagg caacatggcg cggtaacttt cgcgagcttt ctgccagcgt cacgcgaatg
    53161 gcaacctttg cctctagcgg acgcattact ctggacgtgg ttgaagatga gataaaccgt
    53221 ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct gggcgcagag
    53281 gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc aatctgccgc
    53341 caggcaaagt cgctttccgc agccggacgc cagctttttg acgtttcgcg ccagggcaaa
    53401 gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg
    53461 gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag cacctgcatc
    53521 acgcttactg gcggcggggt gtcggtgtag acggcatcta ccatgctgat gctacccata
    53581 ttgaccatcg cgttacggcc aaatttcgag tgatcgacaa ccagcataac gtggcgcgag
    53641 ttctcaataa tggcgcgttt ggtgcgaact tcgtggtaat cgaactccag cagcgaaccg
    53701 tcgctatcga tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa
    53761 tcgagcgtcg cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc accggcgaga
    53821 ataatacgga aatcctcttt taccatcagc gtgttagcaa cgttaagatt gttggtgaca
    53881 atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt ggtgccgata
    53941 tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc gatgcgctct
    54001 ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga actggaaggc
    54061 agcgccgcac cgccatgatg gcgcaggatc aggttttgct ccgccagctc attgaggtcg
    54121 cggcgaatag tctgcgggct gacggagaag tgctccacca gctcctcggt actgacataa
    54181 ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt catttctaaa
    54241 tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc
    54301 cacggctaac ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca aatcaaacca
    54361 tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt aaatgccact
    54421 ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca ccacgccaga
    54481 aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta acagggcgct
    54541 gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg caccgccgag
    54601 ataccaccac cagagcaggt taaagaggat atgcatcagc gagaagtgca ttaacgcgtg
    54661 ggtgaagtaa cgccagaact caaatttcag tgctggatcg aatggccaag ccagccataa
    54721 catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc aggcgatcat
    54781 catcacccag gtcaccggac ctgcgcgttc acgcaaggcg gcaaagaaag gataacggcg
    54841 ataatgcagg ccactgccgg tatggcctgc ctgccagctc gccgccagat aacgcggatc
    54901 tgccgggttt tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct gggactcatc
    54961 cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc
    55021 catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa tcatcaacat
    55081 cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc gccgtacgcc
    55141 acctctgcgg gaaactgacg ttgccaggct tcaaagccgc cgtcaatgct atagaccaca
    55201 tcgtagccct gttgcagcag atactgcgcc gcgcctttgc tgctattgcc gtgataacac
    55261 atcaccatca ccggagtgtc aaagtcgtta ttacgcataa aagcgcccag cgtgtcgttg
    55321 gttaaatgga aagcctgcac cgcatgtccc attgcgaaac tctgtggatc gcgaatatcg
    55381 accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt aatacattcg
    55441 aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta
    55501 cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa
    55561 tgttacctaa agcgcgattc tttgctaata tgttcgataa cgaacattta tgaactttaa
    55621 cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt ggcggcatca
    55681 atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg atgctggagg
    55741 cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac ggtggcctgc
    55801 gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc
    55861 tgctgaaaat ggccccgcat atcgccttcc cgatgcgttt tcgcctgcca catcgtccgc
    55921 atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat ctgggtaaac
    55981 gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg ttaaaaccgg
    56041 aaattaagcg cggattcgaa tattccgact gttgggtaga cgatgcccgt ctggtgctcg
    56101 ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcgtact cgtgctactt
    56161 ctgctcgtcg cgaaaacggc ctgtggattg tggaagcgga agatatcgat accggcaaaa
    56221 aatatagctg gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg aaacagttct
    56281 tcgacgacgg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa ggcagccata
    56341 ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac gaagataaac
    56401 gtattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc accgatgtgg
    56461 aatacaaagg cgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat tacctgctga
    56521 aagtgtataa cgcgcacttt aaaaaacagc ttagccgtga cgatatcgtc tggacctact
    56581 ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt acccgtgatt
    56641 acacccttga tattcatgat gaaaatggca aagcaccgct gctgtcggta ttcggcggta
    56701 agctgaccac ctaccgaaaa ctggcggaac atgcgctgga aaaactaacg ccgtattatc
    56761 agggtattgg cccggcatgg acgaaagaga gcgtgctacc gggtggcgcc atcgagggcg
    56821 accgcgacga ttatgccgct cgcttacgcc gccgttatcc gttcctgacc gaatcgctgg
    56881 cacgtcatta cgcgcgtact tatggcagca acagcgagct gctgctcggc aatgcgggag
    56941 cggtaagcga tcttggggaa gatttcggtc atgagttcta cgaaacggag ctgaaatatc
    57001 tggtcgacca cgagtgggtc cgccgcgccg acgatgccct gtggcgtcgc acaaaacaag
    57061 gcatgtggct gaatgcggat caacaatctc gtgtgagtca gtggctggtg gaatatacgc
    57121 agcagaagtt gtcgctggcg tcgtaaatta acgtaaggtg atcaggtcag atttcaatct
    57181 ggcctgagac tgatgacaaa cacaaaattg cctgatgcgc tacgcttatc aggcctacgt
    57241 ggtttatgca atatgttgaa tttgcatgat tttgtaggcc tgataaggcg ttcacgccgc
    57301 atcaggcatg aacaaagcgc cacgttgtca acaatctgac ctactttttg attcagggaa
    57361 ttaccgcaaa gcccacgcca tcatacccaa cgtaacaaaa cttaagcacg ttccccactc
    57421 actccacttc cgtatctggc ttctattttt gctacgccac tggcaataat atcccaacgc
    57481 aaaaccaagc tgtgcactta tcggaccgac aaagtcgatc cactgttgag accaaaatgg
    57541 cgaaaaattc agtgggagcc agacaactgt cagaaagaaa ctgcgcatat tttccagcaa
    57601 agcaatttgc gctggcgcga cataaaaaat gatattccac aggagcaacc agaaataaac
    57661 tggtgcaaat accagccaac aatgcagtct gcttttcaat tcaggtggcg ttctgcgcgc
    57721 aaattgccaa ccaacgccca ccattgcaca gaagaaaagc gcatattcag ccgataacca
    57781 tagttgtaat tgcagaaggg aaatacgcct gtgcatgagc gggattgaga acagtatttc
    57841 tcccagaaaa ctcagcgtga ttacacaggc agccagcgca accaccgtcg gccacaccaa
    57901 caacggtaca ataacaaagc cgagaaataa cggataaata taaagtctga ttatattcat
    57961 cattattgtc ctgatgattc gggggatgct tcatacagcg gcacataccc cacatcctct
    58021 accaactgtt gcccctgctg acttataaac cagtcaacaa atttctgcgt ttccggcgtg
    58081 ggattttcac gcgtcaccat ataggcatcc acaatgtatg gataagtgcc gttgcggata
    58141 ttttcctcgg taggcgcaac gccatttata gccaaaagtt taatctgctt gcgcattttg
    58201 agcatattgt cattcatacg agtgacgtaa tagtggaagg tgtacccaat ggaagatggc
    58261 gtgttttgat agtcggcaac ttcagtaatt aaaccgccca taagatcaat cacagtggat
    58321 tttttagctg gcagcattgg ggtgtcttgc ataaccaacc ccttcataat cgtttggctt
    58381 ccagaatctt cagggcgctg ccagactttt atatgttcgt cactaccacc tactttattc
    58441 cagcggctag ttttgccgct aaaaatatct ttaacctggt gctcagagag agaatttacc
    58501 gggttattaa tatcaacgat aaagacaaag gcttcgcggg cgaaagcggt atatttcagt
    58561 ttaacacccg cttcttctgc acgttttttc tgtccttccg acggttgtgc aacaaaaata
    58621 accgtcgcgg aacctttaat cagactatcg taggcttcag ctgttcgggt ttgctgcaaa
    58681 ccatacgcct gccaggccag cgaggttcga tccttatctt ttggctcggg tactaattta
    58741 taatacgcgg aggcatacag cgggtataag gctgtagcgc cgtacactct cggccatctt
    58801 gaccaatcat cgctgtaacg taatgcaggt tgcccccgca gagacgtcag tccatggctc
    58861 tcttcttccc tgacgctgtt atagtaccct tcccggtcga cacatcccgc cagtaacgcc
    58921 gtgcctaata gtaaaacacc ggtgaattta ttcatctcgc tgattccttt ctctcgagcg
    58981 ttattccatt gttagataca gcggcacata tcccacatct tctaccagac tctgcccctg
    59041 cggcgttaaa aaccattcga ccagtttttg tgtttctgac gttgtatttt ctctcgtcac
    59101 cataaatgca tcgacgatgt acgcatattt gccgttgcga atgttttccg ccgtcggtgt
    59161 aataccgtta atcgctagca atcttatatt tttatcagca ttcatttgcg tcgcgtaata
    59221 gcggaaggta tagcctattg cgttgtttgt attacggtat tcggcaacga ctttaatcat
    59281 tccctccatc acgcttgcca cttccgtttc ctgcggcgag atcatgcgga cttttttcat
    59341 gacctgtgat tgcatcactg tctggctgcc agagtcttcc gggcgctgcc aggtctggat
    59401 ctcctgatcg ttaccgccaa cagtacgcca attggtaatt gcaccactga aaatgtcacg
    59461 cacctgttgt tcagtcaggg aattaaccgg attatccgca ttgacgatga aaacaaatgc
    59521 ttcacgggca aatggcgtgt atagcaaagt gacgcccgat tcctcagcgc gttttttctg
    59581 cccgccggag ggttgcgcca caaaaataat atcggcatca cctttaacaa tccggttata
    59641 tgcatcgggg gtacgagagt tctccagata ttcccaaacg tgaaaatcct ctggtattac
    59701 acttaatgca taaaatgcag aagcataaat tgggtacgca gccgtggcgc catcgattcg
    59761 cggccagttt tgcctaaact gaatttgcgg tttgccacgc agcggcgtca gtcgattatt
    59821 gagtttatcg ggtcgccagg ccctgatatc aatattttcg cgtacaccga caccaggatt
    59881 ttgctgctta tataaatggg cctgataccc ggcgacaata ccaagtaaga gaagtagaaa
    59941 caggagtaag ttacggcaac gtagtccgga cgtgccggat aaaggatatc tggtcggcca
    60001 ggcgtatccc agcgaaaaac tgatatgcgc cccaacggga ataatgacca tccaccagta
    60061 ctcaccaccc actaatgaga ctaaaaaaac gcccaaccag ggaagcataa ggtttgccaa
    60121 ccaaccagct cccatctggc gaaattgata atctgccaga tgtagcgtga ttatccagcc
    60181 aaccagggtt aatagcgcgg gtaagagaaa aaagcattgc cgccagacgg ctatttctct
    60241 gttttctggc gcgctatcac cggcccacac gcgaccagag aaaaatatta ttatcgcccc
    60301 cagcgtcagc ataaagacgt tgagtagaag ataaattgtt ggcgtgataa ggatattaca
    60361 aacttcaata ataccggtaa gcccgacacc cagcatggca acaaccgctg ccaaaaattg
    60421 cgccagtatg gggatgccga aaaaagtcat tatcagcgag gtcaaaatcc attttctatt
    60481 tggcatcatt ctgtccattc ttcctgaatg acggaattat actccccgag aaccctcgcc
    60541 ccctctggac acttttccga aataaaggcg aaaaaaacgg gaccttgtgg ccccgttctg
    60601 ttttttagcg aacttacaat ctcaccggat cgatatgcca gatatgatca gcgtactctt
    60661 tgatagtacg gtcagaagag aagtagccca tattggcaat gttcagcatc gcttttgcgg
    60721 tccactcttc ctgaagctcg tagagttcat cgactttatc ctgacaatcg acatagctgc
    60781 gataatccgc cagtacctgg tagtgatcgc cgaagttgat cagcgaatca accagatcgc
    60841 gatagcgacc cggatcttcc ggactgaata caccgctgcc gatttgcgtc agcacctgat
    60901 gcagctcctc atctttctcg tagtattcac gcggtttgta gccctgacga cgcagttctt
    60961 ccacttcttc cgctgtgtta ccaaaaataa agatattgtc agcaccgaca tgatccagca
    61021 tctcgacatt cgcaccgtcc aacgtaccga tagtcagcgc accgttaagc gcaaacttca
    61081 tgttactagt gccggaagct tccgtccctg ccagcgaaat ctgttcagac agatctgccg
    61141 ccggaatgat caactgcgcc aggctaacgc tgtagttcgg gatgaacacg actttcagct
    61201 tatcgccaat ctgcggatcg ttgttgatca ctttcgctac gtcattgatc aaatggataa
    61261 tatgcttcgc catgtaatag gccgaagccg ccttaccgcc aaaaatattc acgcgcggta
    61321 cccacttcgc atccaggtcg gccttgatgc ggttatagcg ggtaatcaca tgcaacacat
    61381 tcatcaattg acgtttgtat tcgtgaatac gtttgatttg cacatcgaac aacgcctttg
    61441 gattcaccac cacattcagc tgctgggcga tatactccgc cagacgcttt ttattctcca
    61501 gcttcgcctg atgcacagcg tgattaacca ttgggaaatc acagtgttgt tgcagctcat
    61561 taagcaggct aaggtcggtg cgccagttac ggcccaggtg ttcgtccagc acggctgaaa
    61621 gcgatgggtt cgctaccgcc agccagcgac gcggcgtcac accgttggtg acgttggtga
    61681 aacgacccgg gaagattttc gcaaagtcgg caaacaacga ttgcaccatc agattagagt
    61741 gcagttccga tacaccgtta actttgtggc tcacaacaac cgccagccag gccatacgca
    61801 cacgacgacc gttggattca tcaatgatcg acgcccgtcc cagcagatcg gtatcgttcg
    61861 gatactgttc ctgcaaggtt ttcaggaaat agtcgttgat ttcaaagatg atctgcaggt
    61921 gacgcggcag aattttaccc agcatatcaa ccggccaggt ttccagcgcc tcgctcatca
    61981 gcgtgtggtt agtgtaggag aagacctgac aacacacctc aaacgcgtcg tcccagctaa
    62041 attggtgctc atcgatcagc agacgcatca tctcaggaat cgacagtacc ggatgggtat
    62101 cattgagatg aatcgcgatt ttatccgcca ggttatcgta ggttttatgc aactgataat
    62161 ggcggcttaa aatgtcctga atggtcgagg aaaccaggaa gtattcctga cgcaggcgca
    62221 gctcacgccc ggagtaggtg gagtcatccg gatacagtac gcgagatacg ttctcggagt
    62281 ggtttttatc ttccactgcc gcgaagtagt caccctggtt gaatttaccg aggttaattt
    62341 cgctactggc ttgcgcactc cacaaacgca gcgtgttggt cgcgtcggtg tcgtaaccag
    62401 ggattatctg atcgtaagcg actcccagaa tctcttcggt ttcaatccag cgcgtttttt
    62461 taccttcctg ctgaatgcga ccaccaaaac ggactttata gcgcgtgttg tggcgtttga
    62521 attcccacgg gttaccatat tccagccagt agtcaggcga ctctttctgg ctaccgttaa
    62581 cgatgttctg cttgaacata ccgtagtcat agcggatgcc gtaaccgcgc cccggcaacc
    62641 ctaacgtcgc cagagaatcg aggaagcaag ccgccagacg tcccaggcca ccgttaccga
    62701 ggcccgggtc attttcttca tcaatcagct cttcgagatt taaccccatc gcttccagtg
    62761 cgccctgtac atcttcgtaa attcctagcg acaacatggc gttggagagc gtacggccaa
    62821 tcaaaaactc catcgacagg tagtaaacct gacgagtttc ttgcgacaac tgggcacggt
    62881 ttgaacgtaa ccagcgctcc acgagacgat cgcgcacagc aaataacgtt gcgttcagcc
    62941 attcatgttt attggcgacg accgggtcct ttccaatcgt aaacatcagc ttgtaagcga
    63001 tagagtgctt aagagcttct acgctaagcg tgggcgatga atatgtaaac ggagcattca
    63061 tataggcgtt tcctgaaaac tatttcaagc gatagtaaag ctcacggtac gacttcgccg
    63121 cgacctgcca gctaaaatcc attgccatag cctgacgttg cacaaaccgc cacagagaag
    63181 gacgggacca cagtacaaaa gcacgtcgaa tagcccgtaa cagcgaccag gcattactat
    63241 cttcaaagac aaacccactg gcgacgccat ctgcaaggtt ctcaagagaa cagtcagaaa
    63301 ccgtatcagc aagcccaccg gtgcgccgca ctaacggcag cgtaccgtac ttcaatccat
    63361 aaagttgcgt taagccgcac ggttcaaaac ggctgggcac cagaatgacg tccgcaccgc
    63421 ccataatgcg atgcgaaaat gcttcgtgat agccaatctg aacgcccacc tggccggggt
    63481 attccgctgc cgccgcaagg aaaccttcct gcagcaccgg atcgcccgcg ccgagtagcg
    63541 ccagctgccc gccctgctcc agaagacccg gtaaggcctc cagcaccagg tcgaggcctt
    63601 tctggctggt cagacggctc accactgcaa aaagcggcac tttatcgtca accttaagcc
    63661 ccattgcgat ttgtaactgg cgcttatttt ccgctttatc ttccaacgta tcgcgggtgt
    63721 aacgcgaggc caatagtaag tccgtctctg gactccagat tttctcgtcc acgccgttca
    63781 gtacgccgga aagacgccct tcacgatgac gctgttgcaa caaaccttcc ataccgtagg
    63841 caaactgcgg ttcggtgatc tcgcgagcgt aggttggact gaccgccgta atgtgatcgg
    63901 catagtacag accggccttc aggaaagaga tttgtccgtt gaattccagc ccatgaatat
    63961 taaagaatga ccatggcaat tggatgtcat tcatgtgatg tgcataaaac atgccttgat
    64021 aggccaggtt gtgcacagta aacaccgact tcgccggacg cccgcgcgcc gccagatacg
    64081 caggcgcaag gcctgcatgc cagtcgtgcg catgcaccac atcaggacgc cagaatgggt
    64141 caagcccgct ggccatttct gccccaaccc accccagcag cgcaaaacgc aatacgttgt
    64201 cggtataggc aaataagttg gtatcgtgat acgggcttcc cggacgatca tagagatgcg
    64261 gcgcgtcaat caggtatatg ccaaccccgt tgtaatgacc gaacaacagc gtgatatgcc
    64321 cggcgaaggt atcacgacgg gatactacct gcgcatcggt cacgccacgg cgaatatcgg
    64381 gaaatgcagg caacagtacg cgagcgtcaa cgccgtctgc gatttgtgct gcgggtaatg
    64441 ccccaataac atcagccaga ccgccggttt taagcagcgg gaacatctct gaacatacat
    64501 gtaaaacctg cattatcgct cctgtttatg ccctaacttc cgtagcattt cgcgcgttac
    64561 cagcacgatg ccttcttctg aacgatagaa acgacgtgca tcttcctctg cgttttcacc
    64621 aatcaccatg ccttccggaa taacacaagc acgatcgatg acgcagcggc gcagacggca
    64681 cgagcgacct acccatactt ccggtaacaa tacggcggaa tcaatgttgc agaatgaatt
    64741 cacgcgaacg cgcgagaaca gaacggactg caccaccacc gaaccggaga tcacacaacc
    64801 gccggaaacc agtgagttaa gggtcatccc gtggctaccg gagcgatcct gcacgaattt
    64861 cgctggcggt aatgattcat tgtaggtgcg aattggccaa ttgcgatcgt acatatccag
    64921 ttccggcacc acagaggcca gatcgaggtt cgctttccag taagcttcca gcgtacccac
    64981 atcgcgccag tacggctcgg catccggatc ggattgtacg caagagagcg ggaacgggtg
    65041 cgcataggcc agaccggctt cggtgatctt gggaatcaaa tctttgccaa agtcgtggct
    65101 ggagttctca tcgcgatcgt cttcttccag cagttcatac agatagtcgg cgtcaaagac
    65161 gtagataccc atactcgcca gagatttgct cggatcgttc ggcattgacg gcgggttagc
    65221 aggtttttca acgaattcga taattttatc gttctcatca accgccataa cgccaaatgc
    65281 ggaggcttct tcaatcggta ctggcataca agcaacggtg caacgtgcgc ctttttcgac
    65341 gtgatcgata agcatacgcg agtagtcttg cttgtagata tggtcgcccg ccaggatcac
    65401 cacgtattcc gctttataac ggcgaataat atcgaggttt tgggtgaccg catctgcggt
    65461 gccgcgatac cagttttccc ctttcattct ctgctgtgct ggcagcagat cgacaaactc
    65521 gttcatttct tcattgaaga atgaccagcc gcgctgaatg tgctgcacca gagtgtggga
    65581 ctggtactgg gtgatcacgc ccatacgacg gatcccggag ttgatgcagt tagacagcgc
    65641 aaagtcgata atgcggaact taccgccgaa gtgtacggcc ggttttgctc gcttattggt
    65701 taaatccttc aggcgggtac cacgtcctcc cgccagtatc agggcaacag atttcaatgg
    65761 cagctggcgc gccaacatta agtgatcgtt cttctctaaa ctaaccatga ctaactcctt
    65821 ttttatcatc tctggaacac acacaatccg tgtgcaggtc cctgccagac agccgtaatc
    65881 actgggttat cctctccagc gaatggggga atggcgtgcc actccccagc aggtaaaaca
    65941 atctctgtta cctcaagcgt ggcgttaatt gcgatcaaaa agcgatccga gagcagaatt
    66001 tgcagctgtt tcggcccgtt ttgccactca tccgtgctta aaggttgagc atatcgattt
    66061 agccaacgga cattgccgtc gccttcttcc caccagcgat tctccaccaa agcgggaata
    66121 cgcttgcgca gatggattaa cgcggcggta aatgcggtta aaccactgct tgcctgcgac
    66181 cagtccaacc aggttaattg gttatcctga cagtaggcat tgttattgcc atgctggctg
    66241 tgaccatgtt cgtcaccggc cagtaacatc ggcgtaccct gggagagcaa caacgttgtt
    66301 aacagggcgt gaatgctgtc gcgccgccgt tcaaccaggt caagggtacc gcctacccct
    66361 tctttaccat gattgttact gtaattgttg ttggtcccgt cgcgattttc ttctccgttt
    66421 gcttcattgt gtttatggtt gaaacaaacg cagtcgcgaa gcgtaaaacc gtcatgcgcg
    66481 gtgacgagat taatcgcggc actcggcaga cgaccattac gtttaaaaac atcgctggag
    66541 gcagcaaaac gcccggcaaa cgcccccaga ggcaaatcat aatgtagcca gaaacgacgg
    66601 gcagcatcgc ggaaatgatc gttccactcg gcaaacagcg gcgggaaatt tcccacctga
    66661 taaccaccag gagcgatatc ccacggttca gcaattaact tcacctgcga gagcaccggg
    66721 cagttctgga tagcggtaaa caacggcgca tcctgacgga actctggcgt acggcccatg
    66781 actgccgcca gatcaaagcg gaaaccatcg acgtggcagg tttctaccca ataacgcagg
    66841 caggcgctgg cataatccac caccgccggg tgactcaaat tgagcgtgtt gccacaaccg
    66901 gtccagttgt gataatcgcc gtcttctctt atccaataat agctacggtt gtcgatccca
    66961 cgcagcgaga ataacgggcc gtcgaggtcc agttccgcac tatggttgag cacgatatca
    67021 agaatgactt cgatacccgc tttatgcagt gctttgattg catcgcgaaa ctcatgcagc
    67081 gccgtttctg gcgagcaggc atacgccgga tgcagcgcaa acatcgccac cgggttgtaa
    67141 ccccagtagt tacttagccc catgcgttgc agacgtggtt cactggcaaa ctgcgccact
    67201 ggcagcagtt ccagcgcggt aatgcccaat tgtttcaaat agttgatcat caccggatgc
    67261 ccgagggctt tataagtgcc acggatctcg accgggatct ccgggtgcag gtacgttaat
    67321 cctttaacat gggcttcata aatgatggtg ctgccccacg gcgtgcgcgg cggggcatca
    67381 tcttcccagt catagtgatc aaccaccact acgcatttcg gcgcaatggc ggcgttgtcg
    67441 cgatagtcag gttcattatg accggcgtgc agcagcgggt tatctttaaa ctccccgtca
    67501 atttgccgcg cgcaaggatc aatcaacaac ttcgccgggt taaagcgatg cccctcggcg
    67561 ggttgccagg ggccatgaac gcgataacca taacgcaaac ccgggcgcgc atccggcaga
    67621 taaccgtgcc aaatgtcgcc actgtgccct ggcaagtcat agcgatgttc ctggccattg
    67681 gcgtcaaaga cacacagttc tacccgctcg gcatgagcgg agaaaagtgt gaagttgacg
    67741 ccctgaccgt cgtaatgcgc gccgagggga gcgggtttgc caatggcgag ttgtgtcatt
    67801 ctgcctcccg aaccagccag atagtggcca gcggtggtag cgtcaggctt agtgaatgct
    67861 gacgaccgtg gctggcaatc tcatcgctgt gtaccgtgcc gccattacct gcattactgc
    67921 cgtgatagtg catggaatcg gtattgagga tctcacgcca tttgcctggc tgattaatgc
    67981 cgaagcgata atcatgacgc ggcaccggcg taaagttact ggcaacgatg atttcgttac
    68041 cctctttatc gcgacgcaca aagatcagca ccgagcgttc tttgtcatcc accaccagcc
    68101 attcaaagcc gtacgggtca aaatccagtt catgcatcgc tttatggtgg cggtaggtga
    68161 ggttcagatc gcgcaccaga cgctggacac cgtggtgcca gttatcgccg ccttccaaca
    68221 gatgccagtc gaggctggcg tcatggttcc actcgcggcc ctgggcaaat tcgttcccca
    68281 tgaacagcag tttcttgccc ggaaatgccc acatccagcc atagtaagcg cgcaggttag
    68341 cgaatttctg ccatgcgtcg cccggcatgc ggtcgagaat cgatttttta ccgtggacca
    68401 cttcatcatg cgacaacggc aggacgaagt tttcagtgta gttgtagagc atcccgaagg
    68461 tcagtttatc gtgatgatac tgacgataaa tcgggtcgag cttcatgtag tccagggtgt
    68521 catgcatcca gccgaggttc cacttgtacc agaagcccag accgcccata tcctgcggac
    68581 gagaaacgcc agggaaatcg gtagactcct cagccattgt caccgcaccg gaaacctgct
    68641 caccaagaat acggttggta ttacgcaaga attcaatcgc ttcaagattc tcgcgcccgc
    68701 caaattcgtt cgggatccac tccccctctt tacggctgta gtcacgataa atcatcgacg
    68761 ccaccgcatc gacgcgcagc gcatcaatac caaaacgttc aatccagtaa agcgcgttac
    68821 cgacgaggaa gttactgact tcacggcgac catagttgta aatcagcgtg ttccagtcct
    68881 gatgatagcc ttcgcgcgga tcgctgtgtt catacaagtt cgtaccatca aattcggcaa
    68941 gcgcaaagtc atcagtcggg aagtggcctg gcacccagtc gagaatcacg ttcagaccag
    69001 ctgcgtgtgc agcatcaatg aaataacgga agtcgtcgcg agtaccaaaa cggcgggttg
    69061 gtgcatacag gccggttggc tgataacccc aactgccatc gaagggatgc tcgttaaggg
    69121 gcagtagttc gaggtgggta aagcccatcc atttagcata aggcaccagt tgatcggcca
    69181 gctcgcggta gctcaaccag aaattgttgt cggtgtggcg acgccaggag cccaggtgaa
    69241 cttcataaat agagattggc gcatcaaact gattcgcttt tttgcgctct tcagtctgta
    69301 caaccttttc cggcagcccg caaataagag acgcggtttc cgggcgcatt tgcgcttcga
    69361 aggcataagg gtcggacttc agacgcaagt tgccattggc atcaatcatc tcgtatttat
    69421 agagctgacc gttatgcgcc ccagggataa acagttccca gatgccgctc tctttacgca
    69481 ggcgcatcgg gtgacggcga ccgtcccagt agttgaattg cccaaccacc gagacccgac
    69541 gggcgtttgg agcccagaca gagaaacgcg tacctgtgac gccatccata gtatctgcat
    69601 gcgcgcctaa agtttcatac gggcgcaggt gagtaccttc agataatagc caggcatcca
    69661 tttcctggat tagcggacca aaacggtaag gatcatcaat caggttttgc tgcccatgcc
    69721 agacaacagc caactgatag cggaaaaaat tcttacgtcg cggaatgacg ccactaaaga
    69781 atccccgtga gtcgagacac tccagttttg cgagtttgcg cccggttttc ggttcaatca
    69841 cccacacatc ggtagcgtcg ggtaaaaggg cacggacttc cagtcccgcg gtggttttat
    69901 gcattcccag tacggaaaaa ggatccgcaa aatggcctgc aattagcgcg ttaatcacgt
    69961 ctctatcgat acgatcggac atgcttgtct tcctgtttta ttgtgtcacc ccatccaatt
    70021 ggatttttga cttctggttg tgacattttt ttgacctgaa cggcgcagca ctctgtgcat
    70081 cctctctgcg tcgtcctcac ttcaggtaag gctgtgaata ctcatgtatt cagccaccct
    70141 taaagaatag ccaatgctct atttaactcc cggtaaatca tgaaacatct gcgcttactc
    70201 ctgtattacg cactaacagg ggcggcatcg cgccccagat ttaatgaata aagattacgc
    70261 cagttgacga agcatccgac gcagcggctc cgcggccccc cacagcagct ggtcgcccac
    70321 ggtaaaggct gacaggaact ctggtcccat attcagctta cgcaggcggc ctaccggcgt
    70381 ggtcagcgtg ccggtaacgg cagctggggt tagctcacgc atagtgattt cccgatcgtt
    70441 cggaacgact ttcgcccacg gattgtgcgc agccagcagt tcttccacgg tcggaatcga
    70501 cacatctttt ttcaatttaa tagtgaatgc ctggctgtgg cagcgcaatg ccccgacacg
    70561 cacacataaa ccatctaccg gaattacgga agatgtgttg aggatcttgt tggtttccgc
    70621 ctgccctttc cactcttcgc ggctctgacc gttatcgagc tgtttgtcga tccacggaat
    70681 caggctaccc gccagcggca cgccaaagtt atccaccggc agctcaccgc tacgggttaa
    70741 ggttgtgact ttgcgttcga tatcgagaat agcagaggac ggggtcgcga gttcatctgc
    70801 tacatggcca tacagatggc ccatctgggt taataactca cgcatatgcc gcgcaccacc
    70861 gccggaagcg gcctggtagg ttgcaacgga cacccaatca acaagatcat tggcgaataa
    70921 accacccaac gacatcaaca tcaggcttac ggtacagtta ccgccaacaa aagtcctgat
    70981 gccattattt aatccgtcgg taatgacgtc ctgattgacg gggtcaagaa tgatgatggc
    71041 gtcatctttc atgcgcagag acgatgctgc gtcaatccag taaccctgcc atccgctttc
    71101 acgaagcttt ggatagattt cgttggtata atcgccgccc tgacaggtca caatgatatc
    71161 gagggccttt agcgcctcca gatcaaaggc atcctgaagt gtgccagtgg ttccgccaaa
    71221 agacggcgca gcctgaccaa gctgagaagt agaaaagaag acagggcgaa tggcgtcgaa
    71281 gtcgcgctct tcaaccatgc gttgcatgag aacggagccg accataccgc gccagccgat
    71341 aaaaccaaca tttttcataa gcgttttttc ctgcaaagat gtgtgctgta taaatgtgcc
    71401 ggtctcctct tggcacatct ttcaccatac aaaaagcagc caaagtcgca agtgaaatta
    71461 atcaatgata gcgaagccat cagtaatgcg acttatcctg ctttgttagc acgcagaaag
    71521 tccgcggcaa ttatcaggga atttgagtta tgaatgaaat catttctgca gcagttttat
    71581 tgatcctgat tatggatccg ctcggaaacc tacctatttt catgtccgta ctgaaacata
    71641 ctgaaccgaa aagacggcgg gcaatcatgg tgcgagagtt gcttattgct ctcctggtga
    71701 tgctggtgtt cctgtttgcg ggtgagaaaa ttctggcatt tctcagcctg cgagcagaaa
    71761 ccgtctccat ttctggcggc atcattctgt ttctaattgc cattaagatg attttcccca
    71821 gcgcttcagg aaatagcagc gggcttccgg caggtgaaga gccatttatc gtgccgctgg
    71881 caattccgct ggtcgccggg ccaactattc tcgccacgct gatgttgttg tctcatcagt
    71941 acccgaatca gatgggacat ctggtgattg ctctgctgct ggcctggggc ggcacctttg
    72001 tcatcctgct acagtcttcg ctatttttac gtctgctggg cgagaaaggg gtgaacgcac
    72061 ttgaacgcct gatgggattg attctggtga tgatggcaac ccagatgttc ctcgacggca
    72121 ttcgaatgtg gatgaagggg taaggttcaa acaggtgctg cacacaacca gacgcggcac
    72181 ctgttttatt acctgaactg ataatgacct tcgcccaatc ggataaccct tccctcttct
    72241 tctgcgcggt tcagccacac actaatttgt tcgatggtta aaccagtaca agacgccagc
    72301 ctttcaatac ttatcgattc ctttgcaagg atcgccagtt ccgccacaaa gagttgataa
    72361 aaatcgacag gcgtatacgc gggcattact gcgtaatttg cgtcatcttc atgcagcgaa
    72421 aacagttctg gttgagcgtt acggagtgct ctgtcataca tcagcccagg aacgtctggc
    72481 agtctgatcg tcacattctc agcctgttca gcactccatg acgccccatt ttctactaat
    72541 cgtgaattgg ctgattgcat atcctgattt ggtttaacct gcacaggcag ccactgatgt
    72601 tttaatgcct ccatcgcccc ggttatcgtt ccaccggtca ttcccgcacg tacaaccatt
    72661 gcgctttcag caaggcaata aatatattta tttcgcgcca tcgcattgcc gacggtgaaa
    72721 cgtacctctg ggtaaaacgg cgaaatcaac accaggttgc ctgctataag cccttcacgc
    72781 catttcacta acgtactcgt ttttaacaag ctatcagcta atacgccaac ggcagttccc
    72841 ccggcctcca gtgccgacgc cattgcacat tcatcaatac ctcgcgcacc accagagata
    72901 acgcaaatcc cctgttgggc cagtttcgcg gccagttgtt gggtatagcg caaatcgtca
    72961 gtcggagcat ctcgcgagcc aacaatcgcc ataccttccg cttgcagtaa tgctttattc
    73021 ccgcagccaa ataaaacggg aggtgcatcc gttcgcaatc ggtttttcaa gcgaacggga
    73081 taatcagcat ctccgcgggt taagatccat aaaccggcgc gttgccactt atctaccgcc
    73141 aacgccagac tatgaccacg cgccagcaag ccaagaatac gctcctgaga aatacgcgga
    73201 tcgctccagc ctgtaagttt ctcttgcggt tgcggcacca gaagctcggc gggactgata
    73261 cgttggtgct tcagccataa tgcaaacttc ccccattcac tattactaag aggtttatac
    73321 ttactcgccg ccgcgcgaga aaaatcgctg gttagcagga gagttgcttg tgcattggct
    73381 gaaagattca tcaatttttt accgaggtag aagcaagggc aagaggataa accgtcgggc
    73441 aacctgcctg gcgtaacagt gctgttagaa ctgtcagcgt ccatgcagaa tccacgatat
    73501 cgtcaaccag caacgccgga ccaggcatca aaggaggggt aatcacaaac gccccgtcga
    73561 gattttgaca ctggtggaaa cggttttgct gcattttctg cggtggattg tccacgactt
    73621 tttcaatggc atcaataaaa ggtaagccaa gtttcgccgc cagtcgccgg gcaaaatcag
    73681 gaaccaggtc gaggtgcttt caaaatcagg aaccaggtcg aggtgcttta atgaaggaac
    73741 gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca tttccgcaca
    73801 tgcctccacc agttcatcac tgaatcgccc cgtttttttc tcctgtgcta cctgctttcc
    73861 ccacccggaa tatccccaac gagaaaggat tcgccccgta gatccttgta aactcgcagg
    73921 caagttccct ttaaatccgt attgcgtaaa agccccagct gccacctgtt tattgagatt
    73981 taatgacagg tcagcgtgtt taataaataa gtttgccgca tggagtaagc cgctatcaat
    74041 gtcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaat gttcggcacc
    74101 tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg tgatatagct
    74161 ttcaacgtct gccagctcac tttttctctg attcatcaaa tggttaattc gttcatggtc
    74221 aggagaaaaa ctgacaattg ttctgcgcca taatttctcg gtatacacca caggcgatgg
    74281 attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga gattcgtccg
    74341 ttgttcaatg cctcgtaagg taagaccgtc attcacgcta agtacgttga gtatttcatg
    74401 aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag cacggtcttc
    74461 actgccgcaa agcagtatgc caacagcaga atcgataccg cgaccggcac gccccacctg
    74521 ttagtagtaa ccaactatcg aacctggcat ctggtaatga ataacaaacc ccaaatccgg
    74581 tttgtcaaag cccattccca gcgccgttgt cgcaaccagc actttaattt tatttgccag
    74641 caacgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt cacaagtcac
    74701 gccgctgtag taagcaaatg cactgatacc atttttcctt aaccactcgg cgacaagttc
    74761 ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag ggataaccgt
    74821 tgctaaccat gccagacggg atgagtgttc acctaagact aaggcatcaa gcgccaaact
    74881 ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac ggatatcctc
    74941 aacgacacgg ttattcgctg tcgcggttgt acccagaaca ggggtattcg caggtagttg
    75001 cgcaaaatat ctaatatacg tcggtaatcc gggcgaaaat cgtggcccca atcagagatg
    75061 cagtgcgcct cgtcgaccac aagcaagcca atatgatcgg ctataggata taaaaccgtt
    75121 tcgataaagt cctggtttgc caaacgttcc ggggaaacca gcaagcaatc gacctcacct
    75181 tgcagcaact tatcgctaat gcgctgccat tcttctctgt ttgtagaatt caacgtttca
    75241 gccgtaatac ctaaccgttc agcggcggca acctggttac gcatcagggc aagcaaagga
    75301 gagatgataa ttgtcgggcc agcgccgcga tcgcggaaga ttttactggc gatgaagtac
    75361 acagcacttt tcccccagcc agtgcgttgg acaaccagca gttttcgccg ttggttcacc
    75421 accgcgtcta tggcttccca ttgcccttcc ctgaaagtag cggtggtatt tcctaacatc
    75481 cgctgtaaaa gcaattcagc tgcgtgcttt tccatcttta tctcctgcga tctttaggca
    75541 gtgattagaa cggattattt gcaggtaaat ttgcgagaga gatcaaaaag atgaagagag
    75601 gagaaaagag aaatgaatta ctgcgagcaa gctcgcagta atttgtcgca gtaattaact
    75661 caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga tggtttccat
    75721 catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca
    75781 gaaaccagcg tcgttaacgt ggctgacaac aatcgaacca ccggcgatac aaatcgacag
    75841 cgccgccatt tgcgcaccgg agtagttcag ttgttcaata accggcatca ccagtcccac
    75901 cgccgttaaa caggcaacgg ttgcagaacc ctgaatgatg cgcactgcag cggccagcac
    75961 gaagcaggtg atggcaatcg gcaggcccat gccggttaac gcttcgccca gtgccggacc
    76021 tacgccagag tcaaccagca cctgtttgaa cacgccgccc gcaccaatca ccagcagaat
    76081 gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc
    76141 ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg cggtaaacgg
    76201 atgaccaata aactcgaacc attcgtaagc ggtagagcct tccggcacaa aacgcgcggc
    76261 aatggttttc agccctacca gcaccagcgg cagcaggatc agcgacaggc tgaatccgaa
    76321 ggatggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcagctc
    76381 aacgtaacgg ctgatgaagt taccccacag cggcccggca ataatcattc ccggaattgc
    76441 cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg atgccagcag
    76501 cattggcgct ggccctggca ccaggaacgc cgccgcggcc gccacgcctg cgaataatgg
    76561 gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat
    76621 cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc ccgcaaggcc
    76681 gatggcataa tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg caatctgatc
    76741 gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc
    76801 caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg
    76861 cataccggaa aaaaggccag cccccatgga caccaccatt aaagccagga aagcgtgcat
    76921 acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag
    76981 cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac
    77041 accttccagc ggttgatcga tatccaccac cagtacatcg gtttcgtccg cacccggctc
    77101 ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc
    77161 tttcaggcgg ctttcaatca catcaaaatc gcctttcagg tagatgaaag agagattcgg
    77221 attaccttca cgcagcaagt cacgatagtg ttttttcaat gcagaacaga cgatcagcga
    77281 cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca accacggttt
    77341 gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc gccgtggatg
    77401 gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac ttgcgaccgc
    77461 agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt
    77521 gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt
    77581 aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt
    77641 gtgagctact tcaaatttgt gggcttaaat agatccgccc ggtgacaagg tgaaacctaa
    77701 atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc
    77761 aatactgccc atccgctcac gcggcgtcag cacgctcgca agtcgtggct ccatcacctg
    77821 accaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaactt ttaaccccag
    77881 acgctgacat tcaaacgccg cgccgaccgc cagatcatca ttagtacaga acacgccatc
    77941 cagctgcgga tattcccgcc gtgcctggcg aatcagttca ataccggaag agtaagaaga
    78001 agattgctca accatcacgc tatatggcac caggcctgca tccagcatcg cctgttcgta
    78061 tcccttctgt ttgatgatag tacgttcgtc gagacgtgcg ccgagatagg caatgtggcg
    78121 atgcccgcga gcaataatgg ctgtggtcat ctggcgtgcc gcttcaaagt tatcaaaacc
    78181 gacggcgata tcgagacacg gcgactggct gtccatcaac tccaccaccg ggatccccgc
    78241 cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga tcagtccgtc
    78301 gatattccat gagagcattg attcgagacg ttcttgctcc atttccggtt tataaccgta
    78361 gtgggccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac
    78421 ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtggc
    78481 gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa gagccgcggc
    78541 aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt
    78601 cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat
    78661 cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt
    78721 cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca
    78781 ttgtgctaac gacgctgcaa aaccccggaa gcgtacatca gcacgcgacc ggagcgagcg
    78841 aggaagcctg caaaaaggca ggcttcaaag atgccgttga gctttaaacc ggcggcagat
    78901 cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc tcatcccaga
    78961 ttgccagacc atcgctgatc gaggctttca cgccgttaat ggtgacattg cctttcacca
    79021 cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca
    79081 acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatca cgcgcatctg
    79141 gcgaaagcac cagttgtttg ccctgcactg catcgaagcg acgctgttca taacgcggcg
    79201 taatgccgtt ttcttccggc atgatccaga tctgatacag gtgcagacgc tcggtgctgc
    79261 ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg aactcgcccg
    79321 ccggaacctg ctctttattg cccatgctgt cctggtgctc aacagtacct tccagcacgt
    79381 aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct gcttcaatca
    79441 cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg taatagttgg
    79501 caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg
    79561 ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg
    79621 acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa
    79681 ctattcgagg ggccatcaaa cttacttcgc gagagtcact gtggagggag aggcttgctc
    79741 gaatccacgt tcaagtattt ccagattcgt aagaacttca gattccttga cgtaatttgg
    79801 cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc
    79861 cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac
    79921 cgaatcatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca ggctggtttc
    79981 ctgctggtcg ataccatatt taataaacga acctttctta ccgtgaacga taaatttcgg
    80041 ataatcgatt ttcaccagat ggctggtttt gacgatggct tttagatcgc cataaaacag
    80101 ttgcgcttcg aaagtatcgt caggattggc tttattgcgc aggctgcgga tgtcataagc
    80161 gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc
    80221 atagaacgcg ccatcctgcg gcagcccagg tttggtttct gccaccgggc ggtaatagtc
    80281 aaaatggctt tccacttcga caatctctcc cagcttgccg ctttcaatcg cttttttcgc
    80341 cgtcaggaag caggaatcaa agcgacgatt ctgatatggc gtgacggtca gccctttgct
    80401 tttcgccagt gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac
    80461 cagcacattt ttcccggctt ccatcgcgcg cttcgcgtac tcgaagtggc tgtccgcatg
    80521 ggtgcagaca acaaccagct taacatcggg atcgtttagc acttcgttga gatcgctggt
    80581 gaaatggata tgagagtaaa tgggagcttg ttcttccggc tttgcatggc ggcgaaaaat
    80641 atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt
    80701 gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg
    80761 gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac
    80821 aagcacttcc cgaggataaa ttgagaacga aagatcaaaa aaaaagccag cacccggctg
    80881 gctaagtaat actggaagca atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc
    80941 ttcctgatcg cgagacaata ataatcattc tcattcgcac ttgtccaaca ctttttgcaa
    81001 aaaaatgcat ttgactcgca ttcgaaagtc aatgatgttg aaagggacat ttaccccaaa
    81061 gaggacaaag gaatgagtga gatagtgata cgccatgctg aaacacggga ttacgaggcc
    81121 atcaggcaga ttcacgccca gccggaggtg tattacaaca cactacaggt gcctcatcct
    81181 tccgatcata tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc
    81241 tgtattgatg gagacgtcgt gggccatctc accattgacg tgcaacagcg cccacgccgc
    81301 agtcatgttg ccgattttgg tatctgtgtc gacgcccgct ggaagaatcg cggcgtcgcc
    81361 agcgccctga tgcgagagat gattgaaatg tgcgacaact ggttgcgggt agatcgtatt
    81421 gagctaacgg tgttcgtcga taacgcgcca gcaattaagg tctataaaaa attcggcttt
    81481 gagattgaag ggactggcaa gaagtacgca ttacgtaatg gtgaatatgt tgatgcgtat
    81541 tttatggcgc gggtgaagta aagaatattc tcaaattgcc tgttaccaat gtgaataatt
    81601 agttttgttc atctaatttg agtttttcct tatattacca ccatcatttt gaaacaattt
    81661 gtaacatcaa gagacctttg aatcattact cgttaatatt tcttattaaa g
//
LOCUS       DBFIFS010000003        72354 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.003, whole genome shotgun sequence.
ACCESSION   DBFIFS010000003 DBFIFS010000000
VERSION     DBFIFS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.003"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..577
                     /locus_tag="N7A07_000157"
     CDS             <1..577
                     /locus_tag="N7A07_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2474908.1"
                     /translation="AALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTGMHSVTTD
                     GVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSASYANTSGT
                     LNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLDAEGKGQY
                     VFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            664..1389
                     /locus_tag="N7A07_000158"
     CDS             664..1389
                     /locus_tag="N7A07_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU2474909.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1431..4031
                     /locus_tag="N7A07_000159"
     CDS             1431..4031
                     /locus_tag="N7A07_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HGU2474910.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4045..4593
                     /locus_tag="N7A07_000160"
     CDS             4045..4593
                     /locus_tag="N7A07_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2474911.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4608..5213
                     /locus_tag="N7A07_000161"
     CDS             4608..5213
                     /locus_tag="N7A07_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2474912.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5234..5824
                     /locus_tag="N7A07_000162"
     CDS             5234..5824
                     /locus_tag="N7A07_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU2474913.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5836..6945
                     /locus_tag="N7A07_000163"
     CDS             5836..6945
                     /locus_tag="N7A07_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2474914.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7080..7874
                     /gene="panB"
                     /locus_tag="N7A07_000164"
     CDS             7080..7874
                     /gene="panB"
                     /locus_tag="N7A07_000164"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU2474915.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7886..8737
                     /gene="panC"
                     /locus_tag="N7A07_000165"
     CDS             7886..8737
                     /gene="panC"
                     /locus_tag="N7A07_000165"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU2474916.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9036..9932)
                     /gene="rpnC"
                     /locus_tag="N7A07_000166"
     CDS             complement(9036..9932)
                     /gene="rpnC"
                     /locus_tag="N7A07_000166"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU2474917.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10206..10586
                     /gene="panD"
                     /locus_tag="N7A07_000167"
     CDS             10206..10586
                     /gene="panD"
                     /locus_tag="N7A07_000167"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU2474918.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10590..11819)
                     /gene="yadE"
                     /locus_tag="N7A07_000168"
     CDS             complement(10590..11819)
                     /gene="yadE"
                     /locus_tag="N7A07_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU2474919.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11883..12323)
                     /gene="yadI"
                     /locus_tag="N7A07_000169"
     CDS             complement(11883..12323)
                     /gene="yadI"
                     /locus_tag="N7A07_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU2474920.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12428..13198)
                     /gene="yadH"
                     /locus_tag="N7A07_000170"
     CDS             complement(12428..13198)
                     /gene="yadH"
                     /locus_tag="N7A07_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2474921.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13195..14121)
                     /gene="yadG"
                     /locus_tag="N7A07_000171"
     CDS             complement(13195..14121)
                     /gene="yadG"
                     /locus_tag="N7A07_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2474922.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14230..14892
                     /gene="can"
                     /locus_tag="N7A07_000172"
     CDS             14230..14892
                     /gene="can"
                     /locus_tag="N7A07_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU2474923.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14933..15469)
                     /gene="hpt"
                     /locus_tag="N7A07_000173"
     CDS             complement(14933..15469)
                     /gene="hpt"
                     /locus_tag="N7A07_000173"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU2474924.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15675..18065
                     /gene="gcd"
                     /locus_tag="N7A07_000174"
     CDS             15675..18065
                     /gene="gcd"
                     /locus_tag="N7A07_000174"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU2474925.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18267..19817)
                     /gene="cueO"
                     /locus_tag="N7A07_000175"
     CDS             complement(18267..19817)
                     /gene="cueO"
                     /locus_tag="N7A07_000175"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU2474926.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19983..20330
                     /gene="yacC"
                     /locus_tag="N7A07_000176"
     CDS             19983..20330
                     /gene="yacC"
                     /locus_tag="N7A07_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU2474927.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20436..21302
                     /gene="speE"
                     /locus_tag="N7A07_000177"
     CDS             20436..21302
                     /gene="speE"
                     /locus_tag="N7A07_000177"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU2474928.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21318..22112
                     /gene="speD"
                     /locus_tag="N7A07_000178"
     CDS             21318..22112
                     /gene="speD"
                     /locus_tag="N7A07_000178"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU2474929.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22150..22512)
                     /gene="yacL"
                     /locus_tag="N7A07_000179"
     CDS             complement(22150..22512)
                     /gene="yacL"
                     /locus_tag="N7A07_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU2474930.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22688..25285)
                     /gene="acnB"
                     /locus_tag="N7A07_000180"
     CDS             complement(22688..25285)
                     /gene="acnB"
                     /locus_tag="N7A07_000180"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU2474931.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25640..27493
                     /gene="yacH"
                     /locus_tag="N7A07_000181"
     CDS             25640..27493
                     /gene="yacH"
                     /locus_tag="N7A07_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU2474932.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKAQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27564..28988)
                     /gene="lpdA"
                     /locus_tag="N7A07_000182"
     CDS             complement(27564..28988)
                     /gene="lpdA"
                     /locus_tag="N7A07_000182"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU2474933.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29196..31088)
                     /gene="aceF"
                     /locus_tag="N7A07_000183"
     CDS             complement(29196..31088)
                     /gene="aceF"
                     /locus_tag="N7A07_000183"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU2474934.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31103..33766)
                     /gene="aceE"
                     /locus_tag="N7A07_000184"
     CDS             complement(31103..33766)
                     /gene="aceE"
                     /locus_tag="N7A07_000184"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU2474935.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33927..34691)
                     /gene="pdhR"
                     /locus_tag="N7A07_000185"
     CDS             complement(33927..34691)
                     /gene="pdhR"
                     /locus_tag="N7A07_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU2474936.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35232..36605
                     /gene="aroP"
                     /locus_tag="N7A07_000186"
     CDS             35232..36605
                     /gene="aroP"
                     /locus_tag="N7A07_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU2474937.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36648..37502)
                     /gene="ampE"
                     /locus_tag="N7A07_000187"
     CDS             complement(36648..37502)
                     /gene="ampE"
                     /locus_tag="N7A07_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU2474938.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37499..38050)
                     /gene="ampD"
                     /locus_tag="N7A07_000188"
     CDS             complement(37499..38050)
                     /gene="ampD"
                     /locus_tag="N7A07_000188"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU2474939.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            38138..39031
                     /gene="nadC"
                     /locus_tag="N7A07_000189"
     CDS             38138..39031
                     /gene="nadC"
                     /locus_tag="N7A07_000189"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU2474940.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39234..39674
                     /gene="ppdD"
                     /locus_tag="N7A07_000190"
     CDS             39234..39674
                     /gene="ppdD"
                     /locus_tag="N7A07_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU2474941.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            39684..41069
                     /gene="gspE"
                     /locus_tag="N7A07_000191"
     CDS             39684..41069
                     /gene="gspE"
                     /locus_tag="N7A07_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU2474942.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41059..42261
                     /gene="hofC"
                     /locus_tag="N7A07_000192"
     CDS             41059..42261
                     /gene="hofC"
                     /locus_tag="N7A07_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU2474943.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42296..43339)
                     /gene="guaC"
                     /locus_tag="N7A07_000193"
     CDS             complement(42296..43339)
                     /gene="guaC"
                     /locus_tag="N7A07_000193"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU2474944.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43495..43539)
                     /locus_tag="N7A07_000194"
     CDS             complement(43495..43539)
                     /locus_tag="N7A07_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2474945.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43564..44184
                     /gene="coaE"
                     /locus_tag="N7A07_000195"
     CDS             43564..44184
                     /gene="coaE"
                     /locus_tag="N7A07_000195"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU2474946.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44184..44927
                     /gene="zapD"
                     /locus_tag="N7A07_000196"
     CDS             44184..44927
                     /gene="zapD"
                     /locus_tag="N7A07_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU2474947.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            44937..45134
                     /gene="yacG"
                     /locus_tag="N7A07_000197"
     CDS             44937..45134
                     /gene="yacG"
                     /locus_tag="N7A07_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU2474948.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45225..45623)
                     /gene="mutT"
                     /locus_tag="N7A07_000198"
     CDS             complement(45225..45623)
                     /gene="mutT"
                     /locus_tag="N7A07_000198"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU2474949.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(45683..48388)
                     /gene="secA"
                     /locus_tag="N7A07_000199"
     CDS             complement(45683..48388)
                     /gene="secA"
                     /locus_tag="N7A07_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU2474950.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48450..48962)
                     /gene="secM"
                     /locus_tag="N7A07_000200"
     CDS             complement(48450..48962)
                     /gene="secM"
                     /locus_tag="N7A07_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU2474951.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(49193..50110)
                     /gene="lpxC"
                     /locus_tag="N7A07_000201"
     CDS             complement(49193..50110)
                     /gene="lpxC"
                     /locus_tag="N7A07_000201"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU2474952.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50211..51362)
                     /gene="ftsZ"
                     /locus_tag="N7A07_000202"
     CDS             complement(50211..51362)
                     /gene="ftsZ"
                     /locus_tag="N7A07_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU2474953.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51423..52685)
                     /gene="ftsA"
                     /locus_tag="N7A07_000203"
     CDS             complement(51423..52685)
                     /gene="ftsA"
                     /locus_tag="N7A07_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU2474954.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52682..53512)
                     /gene="ftsQ"
                     /locus_tag="N7A07_000204"
     CDS             complement(52682..53512)
                     /gene="ftsQ"
                     /locus_tag="N7A07_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU2474955.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53514..54434)
                     /gene="ddlB"
                     /locus_tag="N7A07_000205"
     CDS             complement(53514..54434)
                     /gene="ddlB"
                     /locus_tag="N7A07_000205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU2474956.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54427..55902)
                     /gene="murC"
                     /locus_tag="N7A07_000206"
     CDS             complement(54427..55902)
                     /gene="murC"
                     /locus_tag="N7A07_000206"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU2474957.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(55956..57023)
                     /gene="murG"
                     /locus_tag="N7A07_000207"
     CDS             complement(55956..57023)
                     /gene="murG"
                     /locus_tag="N7A07_000207"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU2474958.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57020..58264)
                     /gene="ftsW"
                     /locus_tag="N7A07_000208"
     CDS             complement(57020..58264)
                     /gene="ftsW"
                     /locus_tag="N7A07_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU2474959.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58264..59580)
                     /gene="murD"
                     /locus_tag="N7A07_000209"
     CDS             complement(58264..59580)
                     /gene="murD"
                     /locus_tag="N7A07_000209"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU2474960.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59583..60665)
                     /gene="mraY"
                     /locus_tag="N7A07_000210"
     CDS             complement(59583..60665)
                     /gene="mraY"
                     /locus_tag="N7A07_000210"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU2474961.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60659..62017)
                     /gene="murF"
                     /locus_tag="N7A07_000211"
     CDS             complement(60659..62017)
                     /gene="murF"
                     /locus_tag="N7A07_000211"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU2474962.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62014..63501)
                     /gene="murE"
                     /locus_tag="N7A07_000212"
     CDS             complement(62014..63501)
                     /gene="murE"
                     /locus_tag="N7A07_000212"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU2474963.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63488..65254)
                     /gene="ftsI"
                     /locus_tag="N7A07_000213"
     CDS             complement(63488..65254)
                     /gene="ftsI"
                     /locus_tag="N7A07_000213"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU2474964.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65270..65635)
                     /gene="ftsL"
                     /locus_tag="N7A07_000214"
     CDS             complement(65270..65635)
                     /gene="ftsL"
                     /locus_tag="N7A07_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU2474965.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65632..66573)
                     /gene="rsmH"
                     /locus_tag="N7A07_000215"
     CDS             complement(65632..66573)
                     /gene="rsmH"
                     /locus_tag="N7A07_000215"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU2474966.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66575..67033)
                     /gene="mraZ"
                     /locus_tag="N7A07_000216"
     CDS             complement(66575..67033)
                     /gene="mraZ"
                     /locus_tag="N7A07_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU2474967.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67635..68639)
                     /gene="cra"
                     /locus_tag="N7A07_000217"
     CDS             complement(67635..68639)
                     /gene="cra"
                     /locus_tag="N7A07_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU2474968.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68721..68807)
                     /locus_tag="N7A07_000218"
     CDS             complement(68721..68807)
                     /locus_tag="N7A07_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU2474969.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(68819..69310)
                     /gene="ilvN"
                     /locus_tag="N7A07_000219"
     CDS             complement(68819..69310)
                     /gene="ilvN"
                     /locus_tag="N7A07_000219"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU2474970.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69313..71066)
                     /gene="ilvI"
                     /locus_tag="N7A07_000220"
                     /pseudo
     CDS             complement(69313..71066)
                     /gene="ilvI"
                     /locus_tag="N7A07_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
     gene            complement(71384..72328)
                     /gene="leuO"
                     /locus_tag="N7A07_000221"
     CDS             complement(71384..72328)
                     /gene="leuO"
                     /locus_tag="N7A07_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU2474971.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17665 a        19654 c        17700 g        17335 t
ORIGIN      
        1 agccgctctg gttatggctt ctggttccgc cctggctgta gatggtggtc atatcgactt
       61 taacggtatg gtacagtccg gtacctgtaa agtgggtgtg gtagatactg gtatgcatag
      121 cgttaccact gatggcgtgg ttaccctgga tactgcgaat gttactgata cttttgctga
      181 agttagcgca actgctgtcg gtttactgcc gaaagagttc atgatttctg ttgagtgtga
      241 tccaggtgct ccgaagaatg ctgagttaac tatgggttct gcaagttacg cgaacaccag
      301 cggtaccctg aataacaata tgaacatcac tgttaacggt attgcaccgg ctcagaacgt
      361 aaacattgca gttcataaca tgaaaaacaa agctggcgct gctgaaatta agcaggtcca
      421 tatgaacaac tcttctgaag ttcaggaact gacattagac gcagaaggta aaggccagta
      481 cgtatttaac gcatcttacg ttaaagcacc gaacagcccg gctgtaactg ctggtcatgt
      541 aaccactaac gcgctgtaca ccgttgctta taagtaattg atggtgtgtt gagcataagg
      601 tttgccagca ggcaaacctt attttcgcga atttaatctt tctacagaca gcaaggagtg
      661 cttatgtttc ccttggttaa aaaaacggtt agtgcattat ttgtgagtac tctgttagcc
      721 agtgcaccag cattcgctga cattattatt tccggaacac gtatcattta taacgctgat
      781 aaaaaagatg ttaacgttcg tctggaaaat aaagggaatc gccccttact gattcagaac
      841 tggttggata cgggcgatga caatgccgat cccagccaga tcaaagtgcc gtttaccgcg
      901 accccaccag tttcacgcgt ggagccaaaa cgcggtcaaa ccgtaaaagt gatgtatacc
      961 ggtgcaacag ccctgccgaa agaccgtgaa agcgtctact ggtttaacgt gctggaagtg
     1021 ccgcctaagc cgaaagatgc tgaggctgac aaaaacttgt tacagctggc tttccgcaca
     1081 cgcatcaaac tcttttatcg cccgagtggg ttacagggag aaccggctga agcaccggcg
     1141 aaaatcacct ggaaattaaa caacgctcag ttacaggcaa ataacccaac gccatattac
     1201 gtgtccttta atgaggtcaa gctggaaagc ggcggcaaaa cctacaacgt caattccagc
     1261 atggtcacgc cgttcagcca ggcaagcttt ggcgtaacca gtctgccagg aagcgtgtca
     1321 tccggaaaag tggtctttaa agcgatcaac gactttggcg gcaacattga tggttccgca
     1381 acattctaaa aaataaacgg attcttttta acatcgccag gaatgcaacc ttgtaccagt
     1441 ttactcatca aaaaagccgt atcccgaaaa aaacgctact tgcggcctgt tgtgccctgt
     1501 tttatagcag caacggtgct gcggcggaca ccgtggaata tgacagttcc tttttaatgg
     1561 gaactggcgc atcaacgatt gatgttaaac gttatgctca aggcaacccg acaccgccgg
     1621 gtctctataa tgtccgcgta tttgtaaacg gtcaggcgac ttccagctta gaaattccgt
     1681 ttgtggatat tggcgaaaac agtgcggcgg cctgtcttac ccataaaaac ctggcgcaac
     1741 ttcacattaa gcaacctgaa cagcctgtca ctttactcgc cagagaaggt gaagaagagg
     1801 attgtctgga tctggcaaag tcatacgaaa aggcggatgt gtgctttgac ggtagtgacc
     1861 agtttctcga tctgacgatc cctcaggcct atgttctgaa aagctatggc ggctacgttg
     1921 acccttcttt atgggaatcg ggaattaacg ctgccacact ggcatatacc ctgaacgcgt
     1981 atcacacaag ttcagataac gacaatagtg acagcgtcta tggcgcgttc aactcaggta
     2041 tcaatttagg agcctggcac tttcgtgcgc gcggtaacta taactggaca acagataacg
     2101 gcagcgattt cgatttccag gatcgttact tacagcgtga cattccggca atccgttccc
     2161 agataattat gggtgatgcc tataccaccg gtgaaacgtt tgactctgtc aacgtccgtg
     2221 gtgttcgcct gtacagcgac agccgtatgc tgccttcggc gctggccagt tacgctccga
     2281 ccatccgcgg tgtagcaaac tccaacgcca aagtcaccgt gacgcaaagc ggatataaaa
     2341 tttatgaaac caccgttccg cccggtgaat ttgttataga cgacattagc ccttccggct
     2401 ttggtagcga actggtcgtg accattgaag aagcggatgg ttccaaacgc acctttacgc
     2461 aacccttctc gtcggttgta caaatgcaac gtcctggtgt gggccgttgg gatttcagcg
     2521 cgggtaaagt cattgatgac agtctgcgat ccgaacccaa tatggggcaa gcctcttatt
     2581 actatggtct gaataacctc ttcacgggtt ataccggcat tcagttcacc gataataact
     2641 atcttgccgg gctgttaggt gtgggtatca acaccagcat cggcgccttt gcggtagacg
     2701 ttacccattc ccgtgctgaa attccggatg ataaaaccta ccaggggcaa agttatcgcg
     2761 tgacctggaa caaacttttc caggataccg ggacatcatt taacctcgcg gcgtaccgct
     2821 attccaccca ggattacctg ggcctgcatg atgcgttagt cctcattgac gacgccaagc
     2881 atttgtctgc cgatgaagac aaaaacacca tgcagacgta ctcacgtatg aaaaaccagt
     2941 ttaccgtcag cattaaccag ccattgaata tcgcctatga agattacggt tcgctgttta
     3001 tttccggtag ctggacgtat tactgggcgg cgaacaatag ccgcactgaa tataatgttg
     3061 gttacagtaa aagcgtttcg tggggcagtt tcagcgtcaa cctacaacgt agctggaatg
     3121 aagacggcga gaaagatgac gcgatgtacg tcagcgttag cgtacctatt gagaatattt
     3181 taggtggcaa acgtaagtct tctggtttcc gcaatttaaa tactcagctc aataccgatt
     3241 tcgatggttc acatcagttg aatgttaaca gttccggtaa cactgaaaac aatctggtga
     3301 actacagtgt caacgcaggt tatagcctcg ataaaaacgc cggcgattta gcctctgttg
     3361 gtggttatct caactatgaa tctgggttag gcggtatttc cgcttcggcc tcggccactt
     3421 ctgataacag ccaacagtac tccatctcaa ccgatggcgg ctttgtatta cacagtggtg
     3481 gtttaacgtt cactaacaac agtttcagca gtaacgacac gctggtgtta atcaacgccc
     3541 taggtgctaa aggcgcacga atcaataaca gtaataacga aatcgatcgc tggggatatg
     3601 ccgtgacgtc ctctgtcagc ccatatcgtg aaaaccgggt aggtctgaac attgaaacac
     3661 tggaaaacga tgttgaactg aaaagtacca gcgccaccac cgtaccacgt agcggctccg
     3721 ttgttttgac ccgtttcgaa actgacgagg ggcgttctgc cgtgctgaat attactgccg
     3781 ccaatggcaa atccattccg tttgctgcgg aggtttacca gggtgaggtg atgatcggca
     3841 gcatgggcca gggtggtcag gcatttgtac gcggtattaa cgacagcggg gaattaatcg
     3901 tgcgctggta tgaaaacaac caaaccattg actgtaagtt gcactaccag ttcccggcgc
     3961 agccacaaac gcagggaagc accaacacct tattacttaa caatcttacc tgtcaggtag
     4021 caaatcacta atatggaaaa ttctatgaaa aggatactac taacatccgc gttaataggc
     4081 ctgggtttac cggcggttgg ctctgcaaca gatcttaatg tcgattttac ggccaccgta
     4141 ttagccacca cctgcacgat aactatagtg gaagatggtg gtccagcagt gaccggtagt
     4201 aacgatgaat acagcctgac aattcctgat gttggtctgg ataaagtcgc taccgctgcg
     4261 cccgaagctc aggccaactt taaattaaag gcctccgatt gcagcaatgg ttactcaaag
     4321 atttttacca cacttacagg aacaacggtt tccggtaagt taattgttaa tgaagcaacc
     4381 tctggcgctg ctggggtggg gatggggatt aagcgtagag ataccgcaga ctcgactttc
     4441 ttcacaccca ataatactga caaatttgaa tggagcgccg atgagaaagc cagcggtgtg
     4501 cctctgaccg tagcactgcg cgaaacaacg gcgggagcag gacgcaccgg tgcgtttcag
     4561 gcaaaagcca cctttaactt cacctacgaa taacagggac agccaccatg aaattaaaag
     4621 tcatcgctac actgattgct actgttgccg tgggtgtaag ctttaacagc aattttgctt
     4681 ctgcgagtac aacgtccgct tctttaaccg taaacagtaa cctgactatg ggtacctgca
     4741 gtgctcagat aatggataat agtaataaag tgatcaatga agtggtcttt ggcaatgttt
     4801 atatttctga actcggtgca aaaagcaaag tgcaacagtt taaaattcgc tttagcaatt
     4861 gctctggcct tccccaaaac agcgcccaaa tagtgctggc acctaatggt atatcctgtg
     4921 ctggttctca atcgtcatcg gcgggttttt ctaacaagtt tactgacgct agcgcagcaa
     4981 ccagaacggc tgtggaagta tggactacag atacaccgga aagcaatggc agtacgcaat
     5041 tccattgtgc tcaaaagata ccagtgcctg tgacgcttcc cgccgacacc acaactcagc
     5101 cttacgatta cccgttaagt gcacggatga ccgttgcgga aggtagattg gtaaccgatg
     5161 taagaccggg taatttccgc tctcccacga ctttcacgat cacttatcag taatagcgga
     5221 taaggatatt agaatgctat gcaggcatca taaaatcgta cactttttag ggctggcaac
     5281 agcccttatc acgccttttg cctattccgg ccaggatgtg gatctcaccg caaagatcgt
     5341 gcccagcacc tgtcaggttg aagtcagtaa taatggcgtt gtcgatctcg gcacagtgac
     5401 gctggactat tttgccgaca atgtaacccc gacaacggat tatgcgggtg gcaaaacgtt
     5461 taatgtgaat gtggtctctt gcgataacat tcaaacgaca cagtcgcaaa tgaaattaga
     5521 ttttcagccg caggcgggat cgcttgcaca ggcgaataat caaattttta gtaatgaata
     5581 tgaacagcag gctaccgggg cgaaaaacgt cggcattgtg attttttcgg cccaaccgaa
     5641 tcagcagaca tttaacgtgc gaggaacgga tggttcctca acagcaattt attcggtagc
     5701 acctggcaat gctgttccct caacctggac cttttattcc cgtatgcagc gggtgaataa
     5761 tgcccttcct cctgaatcgg gaatggtgag aagccaggtt attgttaacg tatcttacga
     5821 ataataagga taattatgca aaggaaaggc aataaactgt tgattcagtt atgcagtgtg
     5881 atactgctat tttttaccac atcctggtat gcattggcga atgaatgtta tatagagaga
     5941 aatgctgaag gggattatca catgaagata agctctactc agcttagtct ggcgtcacaa
     6001 atggtcgagg ttccgacaga aatagccgaa gctacatggg atgtaaatat tcaactaaga
     6061 ggcgatgcca tagggtgtaa atctcttggg gatagtaagg cagttcactt tcttaataca
     6121 gctgacccaa gtttaatatc cacgtacacc acaacgaatg gcgcagcgtt attaaaaaca
     6181 actgttccag gcattgtgta ttctgtcgag ttattatgcc ttagttgtgg tgccgcagat
     6241 gaacttgatt tatggctacc tgcacaaagt ggcgcagata acttcatacc aagcacccag
     6301 acgaaatggg cctatgagta cagtgatcaa agttggtatt tacgttttcg cttattcata
     6361 actcctgaat ttaaacccaa gaatggtgtt tccagcggaa caacgatagc aggaaagatt
     6421 gcgtcatggt atataggtac caatgaccag ccgtggatca acttttacat tgacaatgac
     6481 tctttaaagt ttttcgtcga tgaaccgacc tgtgcaacag ttgccctggc acaagatcag
     6541 ggcaacgtca gtggcaatca ggtaacgctt gggaacagct atgtttcgga agtgaaaaat
     6601 gggcttacgc gggaaatccc tttttctatc cgtgctgaat actgttatgc cagtaaaatt
     6661 acggttaagt tgaaagcggc aaataaaccc agcgatgcca cactggtggg taaaacgact
     6721 ggctcggctt caggcgtggc tgtaaaagta aattcaactt atgacaatag caaagtattg
     6781 ttaaaagcag atggtagcaa cacggttgac tacaacttcg ccgcctggtc aaacaacctg
     6841 ctgtttttac cttttacggc gcagctggta ccggatggta gcggtaatgc tgtcggtgtt
     6901 ggaacatttt caggtaacgc gaccttctcc tttacctacg aataagtaaa acgtcattcc
     6961 ctcctcatcg caccagagtt ctgctctggt gcgaacattt ccccacacta ataaccattg
     7021 cctccctgtt ttcggctgtt agaatgcgat caatctgact ttagccatca ggatacgtta
     7081 tgaaaccgac caccattgct tcattgcaga aatgtaagca ggaaaaaaaa cgcttcgcca
     7141 ccatcaccgc ctacgactac agcttcgcaa aactgtttgc cgaggaaggg ctgaacgtga
     7201 tgctggtggg cgactcgctg gggatgaccg ttcaggggca tgaatctacc cttcccgtta
     7261 ccgttgaaga tatcgcctac cacactaccg cagtacgtcg tggcgcgcca aactgcctgc
     7321 tgttggctga cctgccgttt atggcgtatg ccacgccgga acaagccttt gaaaacgccg
     7381 caacggttat gcgtgccggt gctaacatgg tcaaaattga aggcggtgag tggctggtcg
     7441 aaaccgtaaa aatgctgacc gaacgtgccg ttcctgtatg tggtcactta ggtttaaccc
     7501 cacagtcagt gaatattttc ggtggctaca aagttcaggg gcgcggcgat gaagcgagcg
     7561 atcgactgct tagcgatgca ttagccttag aagccgctgg tgcacagttg ctggtgctgg
     7621 aatgcgtgcc ggttgaactg gcaaaacgta ttaccgaagc gctggcgatc ccggttatcg
     7681 gtattggcgc aggcaacgtt accgatgggc agatcctcgt gatgcacgat gcgttcggca
     7741 ttaccggcgg tcatatccct aaatttgcca aaaatttcct cgctgaaacg ggcgacatcc
     7801 gcgctgcggt gcggcagtat atggctgaag tggagtccgg cgtttatccg ggcgaagaac
     7861 acagtttcca ttaaggagtg acgttgtgtt aattatcgaa accctgccac tgctgcgcca
     7921 gcaaattcgc cgcctgcgta tggaaggcaa gcgcgtggcg ctggtaccta ccatgggcaa
     7981 cctgcatgat ggccacatga agctggtcga cgaagccaaa gcccgcgccg atgtggtcgt
     8041 cgtcagtatt ttcgttaacc cgatgcagtt cgaccgcccg gaggacctgg ctcggtaccc
     8101 acgcaccttg caggaagact gcgaaaagct gaacaagcgc aaagtggatc tggttttcgc
     8161 gccctcggta aaagagatct acccgaacgg tactgaaacc catacttacg ttgacgttcc
     8221 agggctttcg accatgctgg aaggcgccag ccggccgggg cattttcgcg gcgtgtcgac
     8281 tatcgtcagc aagctgttta acctggtcca gccggacatc gcctgcttcg gtgaaaagga
     8341 ctttcagcaa ctggcgctga tccgcaaaat ggttgctgat atgggctttg atattgagat
     8401 tgtcggtgtg ccaattatgc gcgccaaaga cggtctggca ctgagttccc gtaacggtta
     8461 tctgacggca gaacaacgca aaattgcgcc cggtctgtac aaagttttaa gttcgattgc
     8521 cgacaaattg caggctggcg aacgggatct cgatgaaatt attgctattg ccgggcaaga
     8581 actgaatgaa aaaggcttcc gcgccgatga tattcagatt cgcgatgccg acacattgct
     8641 ggaagtctct gaaaccagca aacgggcagt aattctggta gccgcctggc ttggcgatgc
     8701 tcgcctgatc gacaacaaaa tggtcgagct ggcgtaatac ttatactcta aataattcaa
     8761 gttgcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg aatttgccca
     8821 gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc aggagcttac
     8881 ataagtaagt gactggggtt agcgaacgca gacgcagcac atgcaatttg aagtatgacg
     8941 agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg tcatggcgat
     9001 ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct atcaatctca
     9061 gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt actcagaaaa
     9121 cgctgggcga attcttgcct ctcctcctgt cttccctgct gaatcccctt ctcaatccct
     9181 ttctcttcaa accactgtgc cagcgtcatc atagacttcc ctcccgtttc cctgtctctc
     9241 agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat atagttttgc
     9301 atggcaacta actgactttc gctagtgtac ccttcgtcaa tcagcgtgac cagttgctcc
     9361 agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag aatcgcaatc
     9421 cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag cgggaaagga
     9481 ctgttataga cgcgtcgcgc cagctccggc gagtaaaaca tatcaaacca gcacattgag
     9541 agcggataag gtgtggcctc gccctgataa aagagaatcg gcactaccag cggtagtttg
     9601 tcgtgatccg cctccagatg ccgatgcatg gcggctatag aataacgcat catgcgaaag
     9661 gccattttct tatccggctt gctttggtgt tcaatcacaa catgcagata accgggattg
     9721 ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttaaggct ctcttcaatg
     9781 aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttcgcgtaa ttccactggc
     9841 aaatgtatct ccagaaagtc gcgagccgtc tccgcatgca ttaaaaattg tttaaatacc
     9901 gcgtcatgcg gtgtggtact cggtgcatcc atcttccctg tcttccgtgt tgtcttgcgt
     9961 gaaggcagta tccagtcaag gcgtgctatc gtcagccacc tctttattgt ttaccgagca
    10021 gcgttcagag aaatatcaat tcgttacagg cgatacatgg cacgcttcgg cgcggtacgc
    10081 aaactgctga tgcaaatgcc gtcagatgag tagacactaa acaaaaatcg ggcaatactg
    10141 cgtgagaatt tcctcaaagc aggcaccgcc tgcttcgtta acgacagggt agaaaggtag
    10201 aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg actcatgcgg
    10261 acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca gccggtattc
    10321 tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc tccacttatg
    10381 ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg gcccactgcg
    10441 ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat gaagaagctc
    10501 gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga
    10561 aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact caccagccgc
    10621 gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa caacggatta
    10681 tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt
    10741 gctgccttca cggctttgtc attaaagccg ccaaacggat acgaaagata ccagacatgc
    10801 ggattaaatt gcgccagagc gcgacgtgaa cgtgcaaaat caaacagaat attgtgctca
    10861 ctacggctca gtaatatggg tcggcgataa ccatctaccc gatgcaaaaa atgggtatgt
    10921 gactggaaat caaatacatc gcgaatttcg ttaagctcag aaacgctcat aaattgcagc
    10981 gattttggtt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat aaacgccgtc
    11041 gccttcatgc catattgttt caacacagga tacgcatagc ggctcactga cttgaggcca
    11101 tcatcaaagg taatcaccac cgctcgcgca ggaagattga tcttattctt cacgtaacct
    11161 tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc catctggtta
    11221 ttgaaagcgc gtaccgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcgtcg
    11281 cgcagaatat ggtgataggt tagcaccgga aggccattat cgggttgggc atccagcgcg
    11341 ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt taacctgtct
    11401 ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccaaa tggcgcactg
    11461 cccacactcg gcgcgttata gaccggcgta tctttccagg taactaagtt ctgattactc
    11521 agcggcttgt tgaggtcacc caaaccgtct tcaacttttt gtcgcccctg aaccggctca
    11581 agatgacctt tatcgataaa ccctttatca aagccaaaat taaatgcgta ataacttgcg
    11641 gcagtgggct ccacggcaat gatttgtccg gcccgaatat tccccacggt caccatcttg
    11701 tcaccaattt gcgcccagac cgcagcattt tcgatggttt gcatataacg ggcaggtaac
    11761 gcggcactga cacttgcggt aaacagcatc agcaggagaa taacagcttg tttgtacatg
    11821 aaaaaatcac cgagggtgaa acaacgcttt tattgtagca aaagcgccat cagaatcgcg
    11881 ggttaatact catacaaatt atgtttgagg acgaaaggcg gatttttttg ttgctggtgc
    11941 caaatactac tcacctccgg cgcacccagt tcgacaatac gctcgcgaaa ctctgaactg
    12001 gtcatggttt cacggcaagc catcatctgt tcaattaacg gtaacgtgac accagaaatc
    12061 acttcgcaac gggagtgttt gtggcttaat aatgaagcca cgcggtacgg tggcgctccg
    12121 gctatatcgg ttaagaagat gacaccctca ccagagtccg cttcatgcag agcatcgcac
    12181 atcatgcggc tgagcatatt agaacttaat ccgcgccaga aattcacggc ccggcactga
    12241 agaagcgccc catgtttttt ctccagcaca tccagtatct cttgcgcccg gtcatcgtga
    12301 caggtaatta cccaacctaa catcgccttc ctccttagcc gaaaatcagt ctaccctgac
    12361 cattgttgat aaagggtgat agccgtcaaa actcctctcc cccaaatcca gaggagagga
    12421 aatagcctta gctacgcaaa ccacgtccac gttggatcag cgaccaacag atcaaataaa
    12481 acgccacaat aaagaccacc agtacgccaa aggtagtgac cagcggaaca tcattgatac
    12541 cgaggaagcc gtagcggaaa ccactgatca tataaacgat tgggttcagg tgcgacagcc
    12601 cttgccagaa cggcggcaac aaagtcagtg agtaaaagac cccacccaaa tacgtgagtg
    12661 gcgttaacac aaaggttggc accaggctga tgtcatcgaa cgttttggca aacacaccgt
    12721 tcagcaaacc cgcaagggag aacaacaccg ccgtgagcac cagggttaag gcaacgaata
    12781 cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat tgccgtcacc agaatgccaa
    12841 caaacagacc acgcgccacg ccaccgccga catatccggc aataatgacg tgagtcggaa
    12901 ccggcgctac cagcagctct tcaatattac gctggaactt ggcaccaaaa aatgatgacg
    12961 cgacgttggc gtaggcgttg gtgatcaccg acatcatgat cagccccggt acgatgaact
    13021 gcatgtagct gaagccatgc atatcgccaa tacgcgaacc aatcaggtta ccgaaaataa
    13081 taaagtaaag ggtcatggtg atgactggcg gcaccagcgt ctgcacccag atacgcataa
    13141 agcgatggat ctctttcgcc cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc
    13201 gcgatctcct tgtttttcat taaccagtga aacaaacagc tcttccagac ggttagcttt
    13261 gttacgcata cttaatacct gaatgccctg ctcacttaac tgcgtaaata cgctgttgat
    13321 cccctgctca cgcagcactt caacttccag cgtcgcggta tcgaccagtc gatactgata
    13381 gccatcgagc ttcggtaacg gacttttcgg tgcgagatcg agaataaagg tttccgattt
    13441 cagcttcgcc agcagcgcct tcatcgaggt attttccacc agctcaccgt gttgaataat
    13501 gccgatattg cggcacagca tttctgcttc ttccaggtag tgtgtggtga gaatgatggt
    13561 ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc
    13621 cacgcctgca gtcggctcgt cgagaatcag tagtttaggt tcatgcatta acgcacgggc
    13681 aatcattaaa cggcgcttca tcccgccaga taacatgcgc gcacgttcgt tgcgttttcc
    13741 ccataaatcg agctgcttaa gatatttttc gctgcggatg tacgcctctt tgcgctccac
    13801 accgtagtac cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt
    13861 aaattcctgc ggcaccagtc ccaactgacg tttagcgttc acgacatctt tctcgagatc
    13921 gtaaccaaat acgctgaccc gcccggaggt tttatttacc agagagctga taataccgat
    13981 agtggtcgat ttcccggccc cgttcggccc gagaagcgca taaaaatcac ccgcttcgac
    14041 ctgcaaatct attccacgaa gcgcctgaac gccgcctgga tagttttttt taagctgttg
    14101 aagttccagt gcaatggtca taaattttta cttaccttac gttcttacac tttatctgtg
    14161 gtttaaatcg tcccggagtt gccctatatt agccaaacgt aattatttgg ttacaggtcg
    14221 ttaacctcca tgaaagacat agatacactc atcagcaaca atgcactatg gtcaaaaatg
    14281 ctggtggaag aggatcccgg gttttttgag aaactggcac aagcgcaaaa accgcgcttt
    14341 ctatggattg gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg
    14401 ggcgaactct ttgttcaccg taatgttgct aacctggtca ttcacaccga cctgaactgc
    14461 ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg aacacattat tatctgtggc
    14521 cactacggtt gcggcggcgt acaagccgca gttgaaaacc cggaactggg gcttatcaac
    14581 aactggctgc tgcatatccg cgatatctgg ttcaaacata gctcattgct cgacgaaatg
    14641 ccgcaagagc gccgtctgga taccttgtgt gaactgaacg tcatggaaca ggtgtataac
    14701 ctgggccact ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt taccattcac
    14761 ggctgggctt atggcattca cgacggcttg ctgcgtgatc tggatgttac tgccaccaac
    14821 cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca acctcaagct gaaacacgcc
    14881 aaccacaaat aaaaatgcca tgccggatgc aacacatccg gcaacttcac acttactcgt
    14941 ccagcagaat cactttgccg atatacggca gatgacggta acgctgtgcg taatcaatgc
    15001 cgtaacccac cacaaactca tccgggatcg agaaaccgat aaattctacc gggacgttca
    15061 cttcacgacg ggacggttta tccagcagcg tacaaatcgc caacgacttc ggttcgcgca
    15121 ggcttaagat ctcacgcact ttcgacagtg tattccccga gtcgatgata tcttcaacaa
    15181 tcagcacatc cttgccacgg atatcttcat ccagatcttt gaggattttc acatcacggg
    15241 tggtggacat gccgctaccg tagctggagg cggtcataaa gtcgacttca tgagatacct
    15301 gaacttcacg gcacaggtcc gccataaaca taaatgagcc acgcagcaga cccaccagca
    15361 ccatatcgct gccgctgtct ttgtaacgct cagtaatctg acgacccagt tcggcgatac
    15421 gcgctttaat ctccgcttcg gggatcatta cttctacagt atgtttcata tctctaacca
    15481 tatgatttaa aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag
    15541 acacgcagta taccagcaaa acgattcatt cgcggaatct gttaataaag ctcaaattgt
    15601 gatgacgatc acacatgtta aaacctataa ttaattgcta ctaaaatatt aatgaattga
    15661 aatggtgtct ctttatggca attaacaata caggctcgcg acgattactc gtcacgctaa
    15721 cagccctttt tgcagcgctt tgcgggctgt atctactcat tggcggaggc tggctggtcg
    15781 cgattggcgg ctcctggtac taccctatcg ctggccttgt gatgctcggc gtcgcctgga
    15841 tgctgtggcg cagtaaacgt gccgcgcttt ggctatacgc cgccctgctg ctcggcacca
    15901 tgatttgggg cgtctgggaa gttggtttcg acttctgggc gctgactccg cgcagcgata
    15961 ttctggtctt cttcggcatc tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc
    16021 ctgccagcgg cgcagttgcc gcactggtgg tggcactgct gattagcggt agtatcctga
    16081 cctgggccgg atttaacgat ccgcaggaaa tcaacggcac cttaagcgcc gatgccacac
    16141 ctgcggaagc tatctcaccc gttgccgatc aggactggcc tgcctatggt cgcaatcagg
    16201 aaggtcaacg cttttcacct ctgaaacaaa ttaacgccga taacgtccat aatctgaaag
    16261 aagcctgggt gttccgtact ggcgatgtga agcagccgaa cgatccgggc gaaatcacca
    16321 atgaagtgac gccgattaaa gtgggcgaca ccctttacct gtgtaccgct caccagcgcc
    16381 tgtttgcgct cgatgccgcc agcggcaaag agaaatggca ttacgatcct gagctgaaaa
    16441 ctaacgagtc tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag
    16501 aaacggcttc gccggaagtg atggcggatt gcccgcgtcg tatcattctt ccggtcaatg
    16561 atggtcgcct gattgcgatt aacgctgaaa acggcaagct gtgcgaaacc ttcgccaata
    16621 aaggcgtgct caatctgcaa agcaatatgc cagacaccaa accgggtctg tatgaaccga
    16681 cttcgccacc gattatcacc gataaaacca tcgtgatggc aggttcagtt accgataact
    16741 tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc
    16801 tgtgggcttt tgatccgggc gcgaaagatc cgaacgcaat cccgtctgac gaacacacct
    16861 ttacctttaa ctcgccaaac tcgtgggcac cagcggccta tgacgcgaag ctggatctgg
    16921 tgtatctgcc gatgggcgtg accacgccgg atatctgggg cggtaaccgc acaccggaac
    16981 aggaacgtta tgccagctcg attctggcgc tgaatgccac aaccgggaaa ttggcgtgga
    17041 gctaccagac cgttcaccac gacctgtggg atatggatct tccggcacag ccgacgctgg
    17101 cggacatcac cgttaatggt cagaaagtgc cagttattta cgctccggcg aaaaccggca
    17161 acatttttgt gctcgatcgt cgtaatggcg aactggtggt tccggcaccg gaaaaaccgg
    17221 ttccccaagg tgctgccaaa ggcgattacg ttacccctac tcaaccattc tcggaactga
    17281 gcttccgtcc gacgaaagat ttgagcggtg cggatatgtg gggagccacc atgtttgacc
    17341 aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga aggcattttc acgccgccat
    17401 ctgaacaggg cacgctggtc ttcccgggta acctgggaat gtttgaatgg ggcgggattt
    17461 ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat ggcactgccg tttgtttcga
    17521 aactgatccc acgcggtccg ggtaacccga tggagcagcc gaaagatgcc aaaggcacgg
    17581 gtacggaatc cggcattcag ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt
    17641 tcctctcacc atttggtctg ccatgtaaac agccagcatg gggttatatc tcggcgctgg
    17701 atctgaaaac taatgaagtg gtgtggaaga aacgtattgg tacgccgcag gacagtatgc
    17761 cgttcccgat gcctgtaccg gtgccgttca atatgggtat gccgatgctg ggcgggccaa
    17821 tctccacggc gggtaacgtg ctgtttatcg ccgctacggc agataactac ctgcgcgctt
    17881 acaacatgag caacggtgaa aaactgtggc agggtcgttt accagcgggt ggtcaggcga
    17941 cgccaatgac ctatgaagtg aatggtaagc agtatgtggt gatctccgca ggcggtcacg
    18001 gttcatttgg tacgaagatg ggtgactata ttgtggctta tgcgctgccg gatgatgtga
    18061 agtaagactt gctcagattg ctgacaacgt gcgcgttgtt catgccggat gcggcgtgaa
    18121 cgccttatcc agcctacaaa attgtgcaaa atcaatggat tgcacaggta acgtaggcct
    18181 gataagcgta gcgcatcagg cagatttgcg tttgtcatca gtttaatgcc cggagagatc
    18241 cgggcatatt cccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    18301 tcatgctcca gcagatggca gtgcgccata taagcacgtt ctttcggtgc gtcgtgatta
    18361 aacttcacca gcacttcact gacattgcct tctaccttaa cggtatcttt ccagcccgcg
    18421 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcgt gccgtgaata
    18481 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtactgccct
    18541 ttcgccgccg caaacatcgg cttattcata tcaaacgcct gaccgttgat tttattggca
    18601 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    18661 cccatcatct ggctgtgatc cattccggcc atcgcctgat cgccatattt ctccattagc
    18721 atctgcatcc ccatcatatc gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc
    18781 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtctggcaa agcaccggaa
    18841 gcactaatag caatcggctg aatccgcatt accggatgag gcttatcaaa cggcgcaatc
    18901 gccatcccca tctggctgac cggcagcgtc accaggtcaa agggtttgtt gtcgttaacc
    18961 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gctcgttcac cttcaccggt
    19021 tcaggtagca gaccaccgtc gctggcaatc acataaagcg ggcgattgtc gctggtggcg
    19081 aaattgagcg agcgggcatt acagccattg agcaaacgca ggcgcagcca accacgcggg
    19141 gcagcgtgtt gcgggtagat tgcaccgttg gtcagcaacg tatcgccaaa ccagcccacg
    19201 gcggcggtca tcacatccag ttgataatca atctgcccgt cggcgctaaa tttcttatcc
    19261 tgaacgatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    19321 tcgtcatctt caatcaccac cagaccagcc agccccatcg ccacctgtcg cccggtcttg
    19381 ccatgttgat gtggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc
    19441 gagcgcttgc cacctggcgg aataattccc tgtgggccgc cgtcgacttc acccggtact
    19501 tccagcccgt gccagtgcaa cgtcgtctct tccgtcagtt ggttatagat atcaaccgtt
    19561 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    19621 gtagttgcag ttttctcgcc aaaggtagac tgacctgcgc caatagttaa ctgaatgcga
    19681 ttacgggcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag
    19741 actgcacggc tccacagtgg caaggctgaa gccacaccca gtgcgacgga atatttcaag
    19801 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    19861 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    19921 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    19981 caatgaagac gtttttcaga acagtgttat tcggcagcct gatggccgtt tgcgcaaaca
    20041 gttacgcgct cagtgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    20101 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    20161 tctttttcgc ccagcaaaac cagtgggatc tcagtaatta cgacaccttc gacatgaaag
    20221 ctctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaagt
    20281 gcaaggccct ggctcgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    20341 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    20401 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    20461 aaacgctaca cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa
    20521 agaccgatca ccaggatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    20581 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    20641 atgttccgct actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    20701 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    20761 tcgatgcggg tgtcgtgtcg ttctgccgtc agtatctacc caaccataac gccggtagct
    20821 acgacgatcc gcgctttaag ctggtgatcg acgatggcgt caatttcgtt aatcaaacca
    20881 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagcc
    20941 ttttcacttc ggcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg
    21001 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc catcgcaaac
    21061 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    21121 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    21181 ttattcaggc gcgttttctc gcctctggcc tgaaatgccg ttattacaat ccggcagtcc
    21241 atacggcagc ttttgcctta ccccagtatc tgcaagacgc actggcttca cagccgtcct
    21301 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    21361 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    21421 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    21481 cctgttccat tatcggggct aatattctta acatcgcccg ccaggattac gaaccacagg
    21541 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    21601 cagaacaccc cggcccactg ccagaaacgg tcgttgccca tctcgataaa agtcatattt
    21661 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    21721 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    21781 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttacc cgcgacatta
    21841 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    21901 atatgaaggc gctgtatgac atggtggatg taaacgtcta tcaggaaaat atcttccata
    21961 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag
    22021 atttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    22081 tttattacgg gcgcaatatg ccagctgttt aacggctctg gcggagctcc caggctccgc
    22141 cagatttatt tacttctgct gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc
    22201 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatcccct cttccatttc
    22261 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcgccgt ccatccatag
    22321 cgtatactca tgccctgccc gctgccagga acgttcgcta cctttcagtg cgtgcgctgc
    22381 ttgttccact tcatcaagca aggccaggtt ttctttcacc tcttcattaa accagtgccc
    22441 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa
    22501 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgca ccgaaatcgc attgcgccat
    22561 tgatgtaatt atcgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    22621 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    22681 tgacttttta aaccgcagtc tggaaaatca ccccatcggc tttctcggtg tactgagaaa
    22741 gctggttgaa gttcagataa cggtaagtat caacggctgt tttatctacc tgcgccacgt
    22801 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gcagccagtt
    22861 ccgcagaagc caggaagaca ttcgcgccag tacccagacg gttcgggaag ttacgggtag
    22921 aggtggaaac caccgttgca ccgtccgcca cacgcgcctg gttacccata cacagggaac
    22981 agccagggat ctcgatacgc gcaccactct taccgaagac gctgtagtag ccttcttcgg
    23041 tcaactgtgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    23101 ctttgtgcgc atccagcagt ttacctgcgg cacggaagtg accgatgtta gtcatgcaag
    23161 aaccgataaa cacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcgt
    23221 ccgggtcgtt cggagcacag aggattggct ctttaatatc cgccagatcg atgtcgatca
    23281 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    23341 ccatgccctg aatacgacgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    23401 acttcagcag gacgatgttg gagttcaggt attcgatgat tggttctttg ttcagcttga
    23461 tggtacaacc ggcagcagaa cgctcggcgg acgcatcggt cagctcaaag gcctgctcaa
    23521 ctttcagatc cggcagacct tcaatttcta ggatgcggcc agagaagatg tttttcttgc
    23581 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    23641 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    23701 gcatatccag cggcattacg cctgttgcgg ctgcaaatgc caccagacca gaacccgccg
    23761 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    23821 gcagcagcat acggttcagc caggagtgga ttacgccgtc acccggacgc agcgacacac
    23881 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    23941 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    24001 ccaggtcttt cagttcatca cgggtcatcg ggcctgtggt gtcctgagag cctacagaag
    24061 tcattttcgg ttcacagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    24121 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    24181 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgaccga
    24241 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    24301 cgaaggtcgc cagcagttcg ccggtttcgt ggttacgtac ttcacctttg tacgggtaaa
    24361 cgtcaattac gtcgcccatg ttcaggttag agacatccac ttcgattggc agcgcacccg
    24421 catcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    24481 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    24541 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    24601 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    24661 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcaa tgggatatcc gggcgtgacc
    24721 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttgaagacgg
    24781 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tcagcccagg
    24841 actgcataac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    24901 agttatcgaa catcagtagt gtgtgagaaa gtgctttggc agcgatcggt gccagtttgg
    24961 catcatccag cgcatcgatc agcggatgaa tgttgtaacc accctgcata gtacccagca
    25021 gttcgatggc tttttccgga gtcagcagtg gggatttggc ttcgcctttg gcgacagcag
    25081 ccaggaagcc tgctttaaca taggcggctt catcgacgcc aggaggaaca cggttggtta
    25141 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    25201 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    25261 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    25321 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    25381 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    25441 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    25501 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    25561 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    25621 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    25681 aatttgcagt gccgggcttt gtgccgcctc tgccgggcta tatataaaaa gccgcacagt
    25741 ggaagcgcct gtggaaacgc aatcgacaca actggctgtg tctgacgctg ccgcagttac
    25801 gcttcctgca acggtttccg cacctcccgt aacacccgcc gtcgtcaaat ccgcattcag
    25861 cactgcacaa atagatcaat gggtcgcgcc cgtcgcgctg tatcccgacg ccctactttc
    25921 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    25981 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    26041 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    26101 gcaatgggtg caaaacctgg gcgatgcttt tctggcccag ccgcaggacg tgatggactc
    26161 ggtacaacga ttgcggcaac tggcacaaca aaccggctcg ctgaagtcat caaccgaaca
    26221 gaaagttatt accacaacga agaaaactgt accggtaaca cagacagtca cggctcccgt
    26281 cataccatcc aataccgttt caactgccaa ccctgtcatt acagagcctg caacaaccgt
    26341 catttccatt gagcccggca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    26401 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    26461 agaaccgttt gttgacagct ttgtacgcgg attcggctat agcatgggcg ttgctaccac
    26521 gtacgcacta ttcagcagca tcgactggga tgacgacgat catgaccatc atcatcatga
    26581 cgatgataat tatcatcacc acgatggcgg tcatcgtgac ggtaatggct ggcaacataa
    26641 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    26701 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    26761 tcaggatatg gcaaagcggt ttcatcaaac tgatgtcaac ggcggaatga gtgccacgca
    26821 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    26881 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    26941 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    27001 actgacccag caacaaaagg acgccgctcg tcagcgttat cagtcagctt ctcctgagca
    27061 gcgccaggca gttcacgaga aaatgcagac taacccgcag aaccagcagc gaagagaggc
    27121 agcgcgtgag cgcattcagc ccgcctcgcc tgagcagcgc caggcagtcc gcgagaaaat
    27181 gcagactaac ccacagatcc agcagcgaag agacgcagcg cgtgagcgta ttcagtcagc
    27241 ctcgcctgag cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    27301 gcaacgtgat aacgcccgcc agcgtgttca atcagcatca cctgaacaac gtcaggtttt
    27361 tcgggagaaa gctcaggaga gccgcccaca acgtctaaac gacagtaacc atactgccag
    27421 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgagcgcg gagcaaggcg
    27481 actggaaagg taaattacag gcgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    27541 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcagta
    27601 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    27661 tggatggtca gtgcgatgtc ttcagcatca caacccattt caattgccag gccgatttca
    27721 cccagcagct cgccgccgtt agtaccgaca atcgcaccac cgatcacacg gtgagattct
    27781 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcgg aagcgatagc acgaccagaa
    27841 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    27901 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    27961 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    28021 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    28081 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    28141 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    28201 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    28261 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    28321 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    28381 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    28441 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtagagtcc
    28501 cagatacgcg gatcttcatg cggaataaac ggcagttgaa tcgggcgaga acccgctgca
    28561 atgatcgcgt tgtcgaagtt gatcacggtt ttaccgttct caccttcaac ttccagggtg
    28621 ttagccccgg taaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    28681 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtcg
    28741 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    28801 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    28861 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    28921 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    28981 gtactcatca tgacctctat atatttatct ccggcgggtc tgacgttttt tttatgacgc
    29041 ccattatcca ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa
    29101 acaacaaacg gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac
    29161 cagaaaaaag ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca
    29221 gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg
    29281 tggtcgaagg agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac
    29341 accggctcca tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc
    29401 ggcgcgaagt gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc
    29461 atttcgcccg cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga
    29521 gacagttcga tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc
    29581 ggggtatcca ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct
    29641 tccgacagcg aactgttgaa gcgaggcatc tgctcaagcg ctgcagcaac ggctttcatg
    29701 atgaagacaa ccggggtgat cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc
    29761 tgtttacgga acgcttccag ctcggtgata tcggttttgt cgaagtgagt aacatgcggg
    29821 atcatcaccc agttacggct caggttcgca ccagaaattt tctggatacg gcccagttcc
    29881 acttcttcga tttcaccaaa cttgctgaag tccaccttcg gccacggcag catgccaggg
    29941 ataccaccgc cagtcgctgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa
    30001 gcctgaacgt cttcgcgcag gatacgacct ttacggccag tgcccttcac tttcgcaagg
    30061 ttaacgccaa actcgcgtgc cagacggcgg atcagcgggg tcgcgtgaac gtaagcgtca
    30121 ttttcagcaa attcagattt gccttccgct ttcgcagccg gtgctgctgc cggggcttca
    30181 gcttttgctg ccggagccgg cgctgctgct tcctgtttcg caggagctgc cgcaggcgct
    30241 gcgccttcaa cttcgaagat cataatcagc gagccagttt tcactttatc gccaacgttg
    30301 actttcagtt ccttcacgac gcctgcaaac ggagccggaa cttccataga agctttatcg
    30361 ccttctacgg taatcagtga ctgttcagcg gcaactttgt cgcccacttt caccatcact
    30421 tcggtcactt caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagcc
    30481 gctggtgcag gggccgctgc cggagccgct tcctgtttag ccgccggagc tgccgcgcct
    30541 gcttcacccg ccacttcgaa gaccataatc agcgagccgg tagacacttt gtcgccggtg
    30601 ttcactttga tttctttcac ggtgcctgca aacggagccg gaacttccat agaagcttta
    30661 tcgccttcta cggtgatcag cgactgctca gcttcaactt tgtcgccaac tttcaccagg
    30721 atttcggtca cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgcg
    30781 gcagcctcgg ggactgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca
    30841 gcgtctgctg caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgagtttta
    30901 tcgccaacag agactttgat ctctttaacg atacccgcct gcggagacgg aacttccata
    30961 gaagctttgt cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact
    31021 ttgaccagga tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg
    31081 atagccatta ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt
    31141 tgaatttggc gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt
    31201 cgcccagcgc cgcaaccacg acataagaag catcaacttc gaagtggtga cgcaggttct
    31261 cacggctgtc ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta
    31321 cgtaagtacg gacctgctca gcgaacagtt tcatatagtc ggtagatgcc acagccggag
    31381 cgtcgttcat cacctgagcg atatacggaa cgcgcggagt ttccagcgga tgcagcatgt
    31441 tccagcgttc acaatcctga ccatcacgcg ccagttcggt gaaggaggtc acgctataaa
    31501 cgtcagaacc tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca
    31561 ggatagaacc ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt
    31621 tgtagatacc tttacggata ccttcctcag caccttccgg cattgccggc atgtggtagt
    31681 tttcgttcag cgtggtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct
    31741 ccagaccgtc atgcataatg acagcaactt cgtaagcgta agccgggtcg taagagatac
    31801 agttcggaat agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac
    31861 cgttcagggt ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc
    31921 cagccgccca gcacagatcg ccgatacgct ggaagccgaa catcgagtaa tagatgtaga
    31981 acggaatcat cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacaac
    32041 ctgcgcccag ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt
    32101 aagcaacctg ctcgcggtcc tgcggggtgt actgctgacc gttcgggctg taaataccaa
    32161 tctgacggaa cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca
    32221 gacgatcttt gatcgacttg ttcttcagca tcacgttcag ggcacgaacg aaagcgatag
    32281 tggtagagat ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggcttggca
    32341 gctcaagctt ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac
    32401 gctgtgcgtg cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt
    32461 tttcgatatc tgcatcagac accggcacat tgaaacggtc gcggatgtga cgcacaccgt
    32521 ccatgttcat tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc
    32581 cgtaaccttt aatggtatga gcaaggatta ccgtcgcttt gcctttggtt tcctgcgctt
    32641 tcttgaatgc agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct
    32701 gctcgtcagt ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt
    32761 cacgaacgta cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt
    32821 tcatcagctg gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac
    32881 cccacatcac tttgatcacg ttccagccag caccttcgaa gataccttcc agttcgttga
    32941 tgatcttgcc gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga
    33001 agaccaggtt atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt
    33061 cgtccatttc accgtcgccg aggaacgcgt aaacggtctg tttagaggta tctttcaggc
    33121 cacggtgttc cagatatttc aggaatttag cctggtaaat agcaccaatc ggacccagac
    33181 ccatagatac ggtcgggaac tgccagaatt ccggcatcaa tttcgggtgc ggataggaag
    33241 agaggccatt gccgtgaact tcctgacgga agttatccag ctgctcttga gtcagacgac
    33301 cttccaggaa agcacgagcg tacacgcccg gggagatgtg gccctggaag taaaccaggt
    33361 cgccgccatc ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg
    33421 ttgcggaaga ctggaaggac gccatgtggc cgcccagttc gaggtctttt ttcgacgcac
    33481 gcagaaccgt catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca
    33541 gattacccgg atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac
    33601 cggtgcctgc ggctacgttg acaccgcctt tgcgggcttc agcaagcagt tggtcgatca
    33661 gatactgagc acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt
    33721 cgcgagtttc gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat
    33781 ctatctaata acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca
    33841 ataagacagg ttctacgttt agttgccgcg cttttatatg cgcttgattt acaacatctt
    33901 ctggataatt tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa
    33961 cgctcacggc ggctttcttc acggcttctg tcgagcaaaa tttcttcgat aaaggccaga
    34021 tggcgatgcg atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg
    34081 cgggtgcggt gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag
    34141 ttctggcgga cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc
    34201 acattgtggg ccgcttcggt gacggcaatc tgatactgga gtacggcgtt tgattccgcg
    34261 tccagatcgc cagactgctg cgccagttct atggcgtggt ggagttcacg gatgcgttcc
    34321 ttgtcttcat cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt
    34381 cgtgtttcga gcaagtcata ctgtgattca ggatggtcgg agagcagctc caccagcgga
    34441 tcgctgaagc tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc
    34501 aacaagccct tcgcttcgag acgttgaatc gcctcacgca atgagggacg ggagacgtca
    34561 aactgttttg ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg
    34621 aggatcaaaa actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg
    34681 ctgtaggcca tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt
    34741 catgtctgtg acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga
    34801 aatcatgaaa ctgtgcacat tttaacaact tgacatatat aacgtttcaa agttgtaact
    34861 atgcacaaat gtagacttta cgtaggaaag ggatttttta acgattatta aactataaaa
    34921 atccgaattg aaccgattca cttaccaatt ttgtgatttt taattcaatt aaaacgaatt
    34981 taaattcatt ctgcatattg agaggggttg aggctgagct ttacaaacgg tttcttttta
    35041 agcaactcat cttcaaccat gcataaagcg ggtgcattcg ctgccgcata ccattattct
    35101 tgatctgacg gaagtctttt tgtaactatt caaacttctt tgatgtaaac aaattaatac
    35161 aacaaacgga attgcaaact tacacacgca tcactgcgta gatcaaaaaa acaatcaccg
    35221 cacgaggttt catgatggaa ggtcaacagc acggcgagca gctaaagcgc ggccttaaaa
    35281 accgccatat tcagcttatc gcgctgggtg gcgcgatagg gacagggtta ttcctgggta
    35341 gcgcctccgt aatacagtcc gcagggccag ggattatcct gggttacgcc attgctggtt
    35401 ttatcgcctt tctgatcatg cgtcagctgg gtgaaatggt ggtcgaagaa cctgtcgcag
    35461 gctcctttag ccactttgct tataaatact ggggcagctt tgctggcttc gcttctggct
    35521 ggaactactg ggtactgtac gttttagttg ccatggcaga gctgactgct gtgggtaaat
    35581 acattcagtt ctggtatccg gaaatcccaa cctgggtttc tgccgccgtg ttctttgtgg
    35641 tgattaacgc catcaacctg accaacgtaa cagtgtttgg tgagatggag ttctggtttg
    35701 ccattatcaa agttattgcg gtagtagcga tgatcatctt cggcggctgg ctgctgttca
    35761 gtggtaacgg cggtccgcag gcaagcgtta gcaacctgtg ggatcagggc ggtttcctgc
    35821 cgcacggctt caccgggctg gtgatgatga tggcgattat catgttctcg ttcggtggtc
    35881 tggaactggt ggggatcacc gcagcagaag ctgataaccc ggagcaaagt atcccgaaag
    35941 caactaacca ggttatctac cgcatcctga ttttctatat tggttcgtta gccgttctgc
    36001 tctcactgat gccgtggacc cgcgttaccg ccgataccag tccgtttgtg ctgatcttcc
    36061 acgagttagg cgataccttt gtggcgaatg cgctgaacat cgtggtactg actgcggcgc
    36121 tctccgtgta caacagctgc gtatattgca acagccgtat gctgtttggt ctggcacaac
    36181 agggtaacgc gccaaaagcg ctggcgtctg tcgataaacg cggcgtaccg gttaacacca
    36241 ttctggtgtc tgcgctggtt acagcattgt gcgtattgat taactatctt gctccggaat
    36301 ccgcatttgg cctgttaatg gcactggtgg tatccgcact ggtgatcaac tgggcgatga
    36361 tcagtctggc gcatatgaag ttccgtcgcg ccaagcagga acaaggcgtg gtaactcgct
    36421 tccctgctct gctttatccg ctgggtaact ggatctgcct gctgtttatg gcggtggtac
    36481 tggtgattat gctgatgacc ccaggaatgg cgatttcggt atacctgatc ccggtatggc
    36541 tggtggtgtt aggtatcggc tatctgttta aagagaaaac cgcaaaagcc gtaaaagcac
    36601 attaatctct ctacgccctc acccgtacag ggtgagggca ataatcttta caccaacgcc
    36661 ccgtaaatcg tcagtagcgc aatcaccacc acgaccacga acgaggtttt cttcgccatt
    36721 gaaaccgctg ccttcggcgt ctccacctta tcgacatgcg gttcacgcgc cagagagaac
    36781 tgcgccagac gcgttaatac ctgatattgc gaagtacgga aatcacccag cgaagcaaac
    36841 caggccggta acgctttctc accatgaccg atcaaggcat ataccacacc tgcaagacga
    36901 accggcaccc aatccagtac atgaagcact gcatcaatgc cggactgtaa acgatgatgc
    36961 ggcgtctggt atcgcgccag ccagtattgc catgcacgca aaaacgcata ccccatcagc
    37021 gtaacgggtc cccaggttcc ccccacaatc agccagaaca gcggtgcaag ataaaaacga
    37081 aagttaatcc acagcaatgc attttgcagc tcacgcaaat actcacgttc gtcgcagcct
    37141 gccgggacgc cgtgaatcat ggtgagttcg ccagccatcg tggcacgggc atggctatca
    37201 ttacgtgaag cagctgtcag ataagcatga taatgaagac gaactttacc tgcgccaata
    37261 cacagcaaac caatcagcag ccacaccagc agcgtgggaa cgttgaacaa tactccctgc
    37321 aatgcgcgta acagtaaaaa agtcacgccc atcgcaataa tggtcatgcc taacgtgcgc
    37381 ccgagagaaa aatgtttcac ccgccgaaag aacgcttcaa gacgatgatc aagctgccag
    37441 tgctcgccca acttaaacag gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc
    37501 atgttgtctc cttgctgacc agcgcacgaa accgtgccca atcaaatgca ggaccgggat
    37561 cggttttccg atccggcgca atatcacaat ggcccgtcat gttattagcg atatccggat
    37621 agcaatcaat cagtgcccgc gtaaccgccg caagctgttg atactgcgca tcggtatacg
    37681 ccagcgtatc ggtgccttca agctcaatcc caatagaaaa atcattgcag cgttcgcgcc
    37741 cctgatactg agagactccc gcatgccatg cacgtttatc gaaaggaaca tactggacta
    37801 tttcaccatc acggcgaatc aaacagtgag cggagacgcg caaatgggcg atctcagcaa
    37861 agaaaggatg tgcctgcgga tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc
    37921 caaactcgcc tggcggcagg ctaatattgt gcaccaccag cagggtgggt gtttcgtcat
    37981 ccgggcggca atcgtaatgt ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt
    38041 ctaacaacat gcaggatctc cttatatgtg gtgctaatac ccggttcaga gtagcatgtt
    38101 tctaccttat gattcgttag ctatctggag ttttaacatg ccgcctcgcc gctataaccc
    38161 tgacacccga cgtgacgagc tgctggaacg cattaatctc gatatccccg gcgcggtggc
    38221 ccaggcgctg cgggaagatt taggcggaac agtcgatgcc aacaatgaca ttacggcaaa
    38281 acttttaccg gaaaattctc gctctcatgc cacggtgatc acccgcgaga atggcgtatt
    38341 ttgcggcaaa cgctgggttg aagaggtgtt tattcaactg gcaggcgacg atgtcaccat
    38401 aatctggcat gtggatgacg gcgatgtcat caacgccaat caacccttgt tcgaacttga
    38461 aggcccatcc cgcgtgctgt taacgggcga acgcactgcg cttaattttg tgcaaaccct
    38521 ttcaggagtt gccagtaagg tacgccacta tgtcgaatta ctggaaggca ccaacacgca
    38581 gttgttggat acgcgtaaaa ccttacccgg tctgcgttca gcgctgaaat acgcggtact
    38641 ttgcggcggc ggagcgaatc accgtctggg gctttctgat gccttcctga tcaaagaaaa
    38701 ccatattatt gcctccggct cagtgcgcca ggcggtcgaa aaagcgtcct ggctgcaccc
    38761 ggatgcgcca gtagaagtcg aagtagagaa tctggaagaa cttgatgaag ccctgaaagc
    38821 aggagccgat atcatcatgc tggataactt cgaaacagaa cagatgcgcg aagccgtcaa
    38881 acgcaccaac ggcaaggcgc tactggaagt gtctggcaac gtcactgaca aaacactgcg
    38941 cgaatttgcc gaaacgggcg tggactttat ctccgtcggt gcgctaacta agcacgtaca
    39001 ggcactcgac ctttcaatgc gttttcgcta gttgctgaca gatttatccg ctccatagtg
    39061 ggcggataat ctccccttcc ccaaccttct tcgtaacgcc tcgcaaattt cttgtctttc
    39121 gctgcaacga aataaaaaac ctcggaagca gctttcccgt ttcgtttttt gcccctcgcc
    39181 tgtgctgatg gcgtttgcca aagtagcacc aaccaaatca aggagcgaaa cagatggaca
    39241 agcaacgcgg ttttacactt atcgaactga tggtggttat tggcatcatt gccattttaa
    39301 gcgccattgg tattcccgct tatcaaaact acctgcgcaa agccgcactc accgacatgc
    39361 tacaaacctt tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa catggtggat
    39421 tagatacctg cgacggtggc agcaatggca ttccctcgcc taccaccacc cgctatgttt
    39481 cagccatgag tgtggcaaag ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatgggc
    39541 taagcgtcgt catgacgcca ggttgggata acgcaaacgg cgtcaccggc tgggcgcgca
    39601 actgcaatat tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc ttccgctttg
    39661 atgacgccaa ctaaggagcg gcaatgaata ttccacagct cacggccctg tgcctgcgtt
    39721 atcagggagt cttgctggat gccagcgaag aagtggttca tgttgcggtg gtcgatgccc
    39781 cctcacatga gttgctggac gcattgcatt tcgctaccac caaacgtatt gagatcacct
    39841 gctggacgcg ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca ttgcccgtag
    39901 ctgttcagga gaagcatcag cccaaagcag agttgctgac tcgaacgtta caatctgcgc
    39961 tggaacaacg cgcgtctgat attcatatcg aaccagcgga caatgcctac cgcatccgct
    40021 tgcgtatcga cggcgtattg catcctttac cggatgtttc accggatgcc ggagtcgcat
    40081 taaccgccag attaaaagtg ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg
    40141 acgggcaatt cactgtcgaa ctggcaggaa acgccgtctc atttcgtatt gcgaccttac
    40201 catgtcgggg tggtgaaaag gtggtattaa ggttgttaca gcaggtgagc caggcactgg
    40261 atgttaacac gctgggaatg cagccgttac aactggcggg ctttgctcat gccttgcaac
    40321 aaccacaggg actggtgctg gtaactggcc ctaccggcag cggcaaaacg gtcacgcttt
    40381 atagtgccct gcaaaagctg aataccgctg acattaatat ttgtagcgtc gaagatccgg
    40441 ttgagatccc catagccgga ctaaaccaga cgcaaatcca tccgcgtgcc ggactcacct
    40501 ttcagggcgt tttgcgtgcg ttattgcgcc aggatcctga cgtcatcatg atcggagaga
    40561 tccgcgatgg cgaaacggca gaaattgcca ttaaagccgc gcaaaccggt cacctggtgt
    40621 tgtctaccct acacactaat tccacctgcg aaacgctggt acgtttacag caaatggggg
    40681 tcgcccgctg gatgctatca tcggcgctta cgctggtaat agcccagcgt ctggtacgca
    40741 aactttgccc acattgtcgc cggcagcaag gggagcccat ccacattcca gtcaatgtat
    40801 ggccgtcgcc gctgccccac tggcaagcac ccggttgtgt acattgctac cacggttttt
    40861 atggtcgcac tgccttattt gaagttctgc ccataacacc ggtcatacgt cagcttattt
    40921 ccgctaatac cgacgttgaa tcgctggaaa cgcacgcccg acaggcgggt atgcgaacgc
    40981 tttttgaaaa cggctgcctg gccgtggagc aaggcttaac cacctttgaa gagttaatcc
    41041 gcgtattggg gatgccgcat ggcgagtaag caactctggc gctggcatgg cataaccggc
    41101 gacggcaatg cgcaagatgg gatgctatgg gcagagagcc gtactttact gcttatggca
    41161 ctacagcaac agatggttac cccactaagc ctgaagcgaa ttgctatcaa ttctgcgcag
    41221 tggcgaggag ataaaagcgc ggaagtcatt catcaactgg cgacgctact taaagccggg
    41281 ttaacgcttt ctgaagggct ggcactgctg gcggaacagc atcccagtaa gcaatggcaa
    41341 gcgttgctgc aatctctggc acacgatctc gaacagggca ttgccttttc caatgcctta
    41401 ttaccctggt cagaggtatt tccgccgctc tatcaggcga tgatccgcac gggtgaactg
    41461 accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc agcaaagagc ccagcgtcag
    41521 ttgaccgaca aagtaaaatc agcgttacgt tatcccatca tcattttagc gatggcaatc
    41581 atggtggttg tggcaatgct gcattttgtt ctgccggagt ttgccgctat ctataagacc
    41641 ttcaacaccc cactaccggc actaacgcag gggatcatga cgctggcaga ctttagtggc
    41701 gaatggggct ggctgctggt gttgttcggc attatgctga cgatagccaa taagttgctg
    41761 atgcgccgcc cgacctggct tatcgcgcgg cagaaattgc tgttacgcat cccgattatg
    41821 gggtcactga tgcggggaca aaaactcacg cagatcttta cgattctggc gctgactcaa
    41881 agtgcaggca ttactttttt acagggcgta gagagcgtca gagaaacaat gcgctgcccg
    41941 tactgggtgc aacttctgac acaaatccag cacgatatca gtaacggtca tcccatctgg
    42001 ctggcgttaa aaaatgccgg tgagtttagc ccgctctgtt tgcaattagt gagaacggga
    42061 gaggcctccg gctcgctgga tctcatgtta gacaacatcg cccatcatca tcgggataac
    42121 acaatggcgc tggcggataa cctagcagcc ttactggaac cggcgttgct gatcataacg
    42181 ggaggaatta tcggtacgct ggtggtggcg atgtatctgc caattttcca tttaggcgat
    42241 gcgatgagtg ggatgggata acgctggcgt gttgctccac accagcgttg gaagattaca
    42301 ggttgttgaa gatgcggttt tcttgttcct gcacacggat aaatgtggtg cgcttggtca
    42361 gttctttcag gcgtgaagcc ccaacgtatg tacaagctga acgcaggccg cccaaaatat
    42421 cacgcgcggt attttcaacc gggcctcgca gcggcagctt aacggtttta ccttctgctg
    42481 cgcgatattc agcaacgccg ccaacgtgac gtttcatcgc agactcggag ctcatgccgt
    42541 agaacagcat aaatttctcg ccgttctcct caacgatgcg accgccgctc tcttcgtggc
    42601 ccgccagcat gccgccaagc atgacgaaat cggcaccgcc accaaaggct ttcgccacat
    42661 cgcccggcgt ggtgcagcca ccatcgctga tgatcattcc gcccagaccg tgcgcagcat
    42721 cggcacattc gattaccgca gaaagttgcg gataaccgac gcctgttttg acgcgagttg
    42781 tacaaacaga acctgggcca atgccaactt taacgatatc ggcacctgag aggataagct
    42841 cctcacacat ttcaccagtc actacgttac cagcacaaat ggttttggtc ggccacgctt
    42901 cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata accattcgcc acgtcaatac
    42961 aaacgaagtt taatgccggg ttcaggtcga gaatctgttt agttttttcg aaatccgcat
    43021 cagacgtacc ggtagaaacc atcacatgtt tcagcacatc agcggaagaa ttgttgataa
    43081 acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc agtcaaaata tcaaaagaag
    43141 ccagcgcaga ggccatagaa aatgtgccta cggtgtccat atttgcggcg ataatcggca
    43201 cgccggacca gctctgacct gaatgtttga aggtgaattg acgttccagt tcaacatcgg
    43261 aacggctttt aagagtggag cgtttagggc ggatgagaac gtctttaaaa cctaacttca
    43321 gatcttcttc aatacgcatg tgcggattcc tggggttaat ggctataagg gtaagagcgc
    43381 aactccagtg acgctatcat acgcactaat caatgcggcg caagactgcg aaattgtctt
    43441 tttttacgct acaatcccat aaatttacct ggtaaatagt gagcgaacgt ttcatcaatt
    43501 tcgctttcgt gcgctcattt ttaacgtttt gataatcatg atgaattccc gggaatataa
    43561 attatgaggt atatagttgc cttaacggga ggcattggca gtggcaagag taccgttgcc
    43621 aatgcgtttg ctgatctcgg aattaacgtc attgatgccg atattattgc gcgtcaggtg
    43681 gttgaaccag gtgcacctgc gctacatgcc attgctgatc actttggcgc taatatgatt
    43741 gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc ggatcttcgc caacccggaa
    43801 gagaaaaact ggcttaacgc cctgctgcat ccgctgattc agcaagagac gcaacaccag
    43861 atccagcaag ctacttcccc ctatgtactg tgggttgtgc cattgctggt agaaaactca
    43921 ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca gcccagaaac gcaacttaag
    43981 cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg aacaaatcct tgctgctcag
    44041 gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca ttgataataa cggcgcaccg
    44101 gatgctatcg catcggatgt tgcccgcctg cacgcacact atttgcagct tgcgtcgcag
    44161 tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct ttttgaacat ccactaaatg
    44221 aaaaaatgcg tacatggctg cgcattgagt ttttgattca gcaactcacc gttaatttac
    44281 ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa tgttagtgaa ttactggatg
    44341 ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga acttgaccgg cagcaacgta
    44401 aactccagac ctggattggc gtgcctggcg tggaccagag ccgtattgaa gcattaattc
    44461 agcagttaaa agcggcgggg agcgtattaa tttccgcgcc gcgtatcggg caatttctgc
    44521 gtgaagatcg tttgattgct ctggtgcgtc agcgactgag catcccaggc ggctgttgca
    44581 gctttgattt acctacattg cacatttggc tgcatctacc ccaggcgcag cgcgactgcc
    44641 aggtagaaac ctggattgcc agcctgaacc cgctcaccca ggcacttacc atggtgctgg
    44701 atttaattcg ccagtcggcc cccttccgta aacaaaccag cctgaatggt ttttatcagg
    44761 ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc gctcgattca cagctttatc
    44821 cgcaaatttc cggacataag agccgttttg ccattcgttt tatgccgctg gacactgaaa
    44881 acggacaggt accggaacgt ctggatttcg aactggcctg ttgctaagga gtaaaaatgt
    44941 cagaaactat tacggtgaat tgcccaacct gcgggaaaac ggtggtgtgg ggtgaaatca
    45001 gcccgtttcg gccattttgc tccaaacgtt gtcagctgat cgacctcgga gaatgggctg
    45061 ctgaagaaaa acgaatcccc agcagtggcg atctttccga aagcgatgac tggagcgaag
    45121 agccaaagca gtgacatttg tctgatgccg cacgtaggcc tgataagacg cggacagcgt
    45181 cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa cgccttatcc gacatacaca
    45241 cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc attaagaccg
    45301 actagcgaca tccacttacc gggttgccct tctttacccc acggctcccc ttcccagctt
    45361 tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatattc cagtttttca
    45421 aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct gaagttcacg caccaccgcc
    45481 tgttccggcg tttcacccat ttcaatttta ccgccgggaa actccagttt attcgccatg
    45541 tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg aataatacct
    45601 accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag aatcctgcgc
    45661 cttttacttc aacagttagc ttttattgca ggcggccatg gcactgcttg tattttttac
    45721 cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctcaccggtt tgcgccgcca
    45781 gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt tgcgctaaac
    45841 gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc atacgtacct
    45901 gaactttgct cagcgtactg ataacttcat atttcaacga ctccagcatc gctgcaaaca
    45961 tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag ccacgcaggt
    46021 ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg gagtcaagcg
    46081 tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc acttcttctt
    46141 tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc tcttcatgca
    46201 gttctggttc tttatccagc cactcagcaa ttggtaaatc gaggtcgaaa tcgttcttca
    46261 gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc ggaatgtagg
    46321 catcaatggt cgctttgaac acatcttcac gaatgctgtt aatggtttcg ctcacatcgc
    46381 tgacatccag caattcgtta cgctgggagt aaatggcgcg acgctgatcg ttagccacgt
    46441 catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact ttacgctggg
    46501 cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc ttcataccca
    46561 gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc gcatcttcca
    46621 tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga ccgcgcaact
    46681 ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg ccacctgctg
    46741 ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt tgctctgcgg
    46801 tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca atatctgtac
    46861 cacgacccgc catgttggtc gcgatagtca ccgcagccgg ataacctgcc tgagcaacaa
    46921 tcgccgcttc gttggcgtgg aatttggcgt tcaggacatt gtgcttaata ccggctttgg
    46981 tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc agcaccggct
    47041 ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt tccgcttcag
    47101 tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc ggaacaacga
    47161 cggtatccag tttgtagatg gagctaaatt cgaaagcttc ggtatcagca gtaccggtca
    47221 tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa gccagcgttt
    47281 ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc agaccatcgg
    47341 accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata acttcaccat
    47401 ctttaacgat gtagtcgacg tcacgggtaa acagcgcatg agcgcgcagc gccgccgtta
    47461 cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca tccatgatgc
    47521 cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg ttcacctggc
    47581 gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag tcttcttttt
    47641 cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc gagctgtctt
    47701 ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg gagtccactt
    47761 cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg aacgccatat
    47821 tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg tcagctgcgt
    47881 aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccgaca gtcaggccaa
    47941 ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag tcgttgacgg
    48001 taactacatg cacgccttta ccggttagtg cgttcagata agcaggcagc gttgcggtca
    48061 gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga accataccgc
    48121 cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc tcacgtacca
    48181 cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga cgtgcacgaa
    48241 actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc ggttccatgg
    48301 cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta cgactaccga
    48361 aaactttagt taataatttg attagcataa taaaatctca aacgccccgc gttgcggagt
    48421 taataaaatg aagtaaaggt ttattgttgt tagctgaggc gttgagggcc agcacggatg
    48481 ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt aaaatgcgca
    48541 taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag cgcgctgagc
    48601 gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc aacgggcagc
    48661 gtttgcggtg ccattgcgaa ggaaagatga cggattaccg tgcgaatggc atgttgatgc
    48721 cagtaatcta cggaatagtt cgaattcggg cggcgcgtgt tcgcttccag caaggccaat
    48781 tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt tgcgggcgcg
    48841 tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcaac catccctaat
    48901 aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag tattccactc
    48961 acgttattgc catcccgatc gtattcaaac gtatttagcg cacaaatctt atcattaaag
    49021 cctgaagtcc acagcacgaa tgaagataag ttgtccgtaa aagtgcttaa gaaaacagcg
    49081 ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt tcacctggcc
    49141 ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt cgttatgcca
    49201 gtacggctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg aaggtcacat
    49261 attcccaggc ttcctgtttc gccaggacag cctgcagcag tttgttattc agtgcatgac
    49321 cggatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac aagtcaccga
    49381 tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg tcttcgttca
    49441 gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg cacaaaccac
    49501 gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg ctgatctggc
    49561 gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa tcaatagccg
    49621 gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta aattcagccc
    49681 acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt ttggcgcagt
    49741 tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg ctgccgtcca
    49801 tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc aagcccgcga
    49861 gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg accagacacg
    49921 tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc ggtggattca
    49981 agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac gtcagggtga
    50041 ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta agtgtccttt
    50101 gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac tatacattcg
    50161 gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc ttaatcagct
    50221 tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt ttgcggcgca
    50281 ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc catcccatgc
    50341 tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag agtgatttca
    50401 ggacgtttgt ccatgccgat acctgtcgca acaacggtta cgcgcagctc gtcattcata
    50461 tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa tgcacggatg
    50521 gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgctgt gatgttaacc
    50581 agcacgccgc gtgcgccaga caggtcgata tcttccagca gcggagaaga gatagccatt
    50641 tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat cattgcgtag
    50701 cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa acccggacga
    50761 gtaatcagtt cggcgatgcc ttgcacagcg cctttgagta catcgttcgc tgcgccaaac
    50821 gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt cgggatagtg
    50881 atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa tgccatacgc
    50941 ttcttgcctt caaagttgaa gggcttagtg acgacagcaa cggtcaggat acccaaatct
    51001 tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc catacccgca
    51061 gcaataaaga ccatgtctgc accttccagc gccgcacgca gtgcatcgcg atcctcatct
    51121 gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt gataccgcta
    51181 ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc ggtatttacc
    51241 gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc attaccgccg
    51301 ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat tggttcaaac
    51361 atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg gcctcataaa
    51421 aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc aactgatgct
    51481 gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc atagtgaagc
    51541 aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc ggtaatgttc
    51601 agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc tgcaagacct
    51661 tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg tttaacccct
    51721 tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt gagcagctcg
    51781 gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg cggcggacga
    51841 ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc cagcgcacaa
    51901 ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc gtaagcgata
    51961 tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa tgccccaccg
    52021 gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac acccagttca
    52081 cgttcatctt ccgtcaatac cgaataactt gatgccagac cggcaaatat cagttggtca
    52141 actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc gttgtggcat
    52201 gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gccctaccgg attcttgatc
    52261 ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg atgctcatcg
    52321 cgtacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg cgttacttct
    52381 tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt accagaaagc
    52441 gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc aatggcacgt
    52501 tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat accacgcgac
    52561 gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc ccctactaaa
    52621 gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc cgtcgccttg
    52681 atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct ggcggcaagg
    52741 gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta atccgtttgc
    52801 catcggtttg cgcctgctgc tgtagaaccg gataaagttc tacaaagcga gccaaacgtt
    52861 tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc aactgccagg
    52921 aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc agcatctgcc
    52981 ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca tacagcattg
    53041 gaagcacctg cttgctggtg cgatctggcg gcacgctgaa ggtatttcct tccgcgtcta
    53101 ccatgtgttg atcattccac cgcgcaatcg gcacatattc aaccagatga atcttcaatt
    53161 catcaggcca ctgctttctg acgctcacct gcttaatcca cggcagacgt tgttctattt
    53221 gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc aatgccagga
    53281 tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac accagctttg
    53341 agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg ctcaccaaca
    53401 ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca ttattgcggc
    53461 gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac atattagtcc
    53521 gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg acgtgccgcc
    53581 atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag cagataaaac
    53641 tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc taacgtcgtc
    53701 catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag acctgcgggg
    53761 cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa ggttccggac
    53821 ggttgaatac gtactgacgg taaaatttct tcaccgagta tcgcaaccgt gaactccggc
    53881 ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa tcttaatgca
    53941 tcttgtagag cattttctgc tactactttt gacattccca cactggaacc ttcgcggctc
    54001 ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt atcactcagg
    54061 cctttttcaa actctacgcg ggttaacgct acccacggcg cgaccggtaa accggcacct
    54121 tgccacagaa gtttgctgcg tagtttatcc attgaaagcg cagatgccat cactccgctg
    54181 ccggtataag gcaagcccat cagctcgagc atcccctgca gcgtaccatc ttcaccgccg
    54241 cggccgtgta gcgcgataaa cactttctga aagcccatcg acttcagttg cgtcacgtca
    54301 acttctttcg ggtcgacagg atatgcgtca ataccgcctt cacgcagccc ggctaacacc
    54361 gctgcgccag aattcagaga aacttcccgc tcagcggaag tcccgcccaa caggaccgcg
    54421 attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga tttcagctaa
    54481 agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt cgttaccggt
    54541 taataccggt gccagcatct cggctacctg cgcagggtcc ggcaccagaa tgggatctat
    54601 tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgcacccg gaattggcgc
    54661 ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca gcacattggc
    54721 gaaatcatca tacaggtcgc gtgtacgggt aaaacggtgc ggctgaaaca gcattaccag
    54781 gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg gcgtccactt ccgtcgggtg
    54841 gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg gctccagcgg
    54901 gaattcaccg aggaaatcaa aacggcgacc agtcccctgg aagctctcca gcgcacgcaa
    54961 aatagcctcg tcgtcaatgc cctcttccgt agcaaccgca accgcagctg cggcgttcag
    55021 cgcgttatga cggcctggcg cattcagggt gacgcgcatc ggctctttgt cctggcgcag
    55081 cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca cgtcggcatc
    55141 ttcgctgaag ccgtaagttg tggtctgacg tcccactcgc ggtaacaatt cgcggatcac
    55201 cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca gaaaattaat
    55261 aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt cggcttcgat
    55321 attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac tctcatctgc
    55381 ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg cttttaccag
    55441 cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg agaccatcgc
    55501 ggtagttgtc gttttgccgt gcgttcctgc aatggcgatg ccatgacgaa aacgcattaa
    55561 ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag ctgcgacaat
    55621 ttccgggtta tcggcagaaa tggcgctgga aacaacgacc acgctggcat cacgtacgtt
    55681 ttccgggcga tggttgaaat aaatcgtcgc tcccagattc attaactgct gcgtgaccgg
    55741 atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa cttcggcaat
    55801 accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc gacgcatttc
    55861 gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg ccattaactt
    55921 cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc ccgactcact
    55981 tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg ttctgccatg
    56041 gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc cacgctaagc
    56101 tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc attccagtat
    56161 tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc cgcggcgatt
    56221 tcactcaccg ttaaagcacc ggagcggcaa acgacgacat ccgcccacgc atacgccgcc
    56281 gccatatcat caataaattc agtcacttta tgctgcggtt gccccgcttc ggcatacgcc
    56341 tgttcaacgg attgttgcga acctttgccg ctctgatgcc agatagtgac tgaatcaccc
    56401 agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg ggaaccaccc
    56461 actaccagca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg caacggtagc
    56521 gccaacacat cggtacgcac cgggttaccc accacttccg cattggggaa agcacctgga
    56581 aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa gcccgcaata
    56641 ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag accaccagga
    56701 cctgatacgt agccgcccat accgagcacc acgtcaggtt tgtacgcttt cataatcgcc
    56761 cgcgcctgac gccaggcatt gaagatacgc agcggcgcag ctatcagtgc ttttatacct
    56821 tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt tggcactaag
    56881 tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg agccattaga
    56941 tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc catcaccatt
    57001 aatcgctttc cttgagcact catcgtgaac ctcgtacaaa cgcctgcgct ttctccagac
    57061 gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc agtaagctcg
    57121 aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc cccgccgccg
    57181 cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag gcgagaaaac
    57241 cggaaaaacg gtggtcaatt tctaatgctt tacggccaat cgacatcgcg cgaaaagcga
    57301 cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct tcgccgataa
    57361 tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt acagagttac
    57421 ctaaaccttg tccccacagt tcgccgcgac caaacgccat cagcgattgc gttaactgat
    57481 agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg cggatacggt
    57541 acggttcggc gagtatcagc aacacaaccg ctgaaatgcc cataccgata atggcaatga
    57601 actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca aacaacacca
    57661 ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga atcacgccca
    57721 tcggtttcag gaagccgcgc aggttattac gtacttcgtc acctttacgc accagatagt
    57781 tggcgatata gcaaaacagc gacagttttg tcagctcagc aggctggata cgcagcaaac
    57841 cgagatcgat ccaacgcgat gcccctttaa ccgagctccc cactaccagc acgatcatca
    57901 gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac tccatcggca
    57961 gacgcagcgt aatgatcgcc agaataaacg ccaaaatcag atagacacca tcacgcttcg
    58021 cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc gaggtcacca
    58081 tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga tcgtacatga
    58141 tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc gccgtggaga
    58201 tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg agagataaac
    58261 gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt caaagttctt
    58321 gaactgatca aggctggcac aggccgggga gagtagaacc atatcgcccg gctgaacacg
    58381 cggagccagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca cttccgggcg
    58441 tagcgccgcc agctgcacgc cgtcacgacc gaaacaatac agacgtacgt tatcgccatt
    58501 caggtaacgc gccagtgggc taaagtccgc cgatttacca tcgccgccca gcaacaaatg
    58561 cagcgtgccg tctacgtgca ggccattcag cgccgcttcc gtactgccga cgttggtcgc
    58621 tttcgaatcg ttaatccaac gtacgccgtt atgctccagc acaacttcaa agcgatgcgg
    58681 cagaccagtg aatgtggtta acgctttcag gctgctggca cgcggtaacc ctgcggcatc
    58741 tgccagcgcc agcgccgcca gcgcattggt gtagttatgc tgcccggaaa gtttcatttc
    58801 tttcacgttc agcactttct cgcctttcac ccgcagccag gtttcgccct gctgatgatt
    58861 caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatccg caccgcgaat
    58921 cggcattgtt aaggcatcat cagcattgac gacacaaact ttcgcatttt cgtaaatacg
    58981 caattttgcc gcacggtatt gctgtaaacc aaacggatag cgatccatat gatcttcagt
    59041 cacgttcaga atggtcgctg ctaccgcctg taagctggag gtggtttcca gctggaagct
    59101 cgacagttcc agtacgtaca gttcgcactc atcatccagc agcatcaacg cgggcaggcc
    59161 aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac ccactagcgt
    59221 ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt gtgcttcgcg
    59281 acagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag cgcttaagga
    59341 tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca tcagccattc
    59401 atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca ggccaggcgg
    59461 tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa agtccacgca
    59521 ggaaagcccg gtgaggccca ggccgataat gacaacattt ttaccctggt aatcagccat
    59581 gattaacgta ccttcagcgt tgccagacca atcaggacca gcatcagcga aataatccag
    59641 aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg atgaatcggt
    59701 gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag gatgacggaa
    59761 agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg acgcagcagt
    59821 acggcgataa tgcctaacgc accacctaac gccagcgaac ctacatcgcc cataaagacc
    59881 tgcgccggat aggtgttaaa ccacaggaag cccagccctg ccccgactat cgcggtacag
    59941 acaataacca gttccccggc gtgtcgcaga tatggtatgt gtaaatagct ggcaaagttc
    60001 atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac ggtcggcata
    60061 attgccagac cgtcaagacc atcggtcagg tttaccgcgt tgccagtacc cacaatgacg
    60121 aagtaagcca gcagaatgta gaacagcccc agttgcggca tcacatcttt aaagaatggg
    60181 accaccagct gcgttgcggg cgtgtctttg ccaacaaggt acagcgcgaa ggcgacaccc
    60241 agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt ggtgtcttta
    60301 cgcactactt tgcgataatc atcaacaaag ccaataacac cgtaacctac cagcaccacc
    60361 aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga gatcacaatc
    60421 gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa gtgtgattca
    60481 ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc aatcatacgc
    60541 gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc gcgaaacgtc
    60601 agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc cagccaaact
    60661 aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca ctacgtgaac
    60721 ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga cgcgtaatta
    60781 acgcagtttt ctcagcaaaa tgttcgccaa cgccgctggc ggtgctgata gcatggcttt
    60841 gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg cccacctgta
    60901 catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc agcacgcggt
    60961 agcccggcat ttcagccagt acctgaacag cggcggtcat tgaaccgaca ttggcgttgt
    61021 aggagtcgtc gagcagcaac tggttttctg ccagttgaat ggggaacaaa cggcctggaa
    61081 cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc acggacatgg
    61141 agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac ggcagcagaa
    61201 catccacgct accggttggg gtttgcaggg taaattccgt accgtgcgaa gtcacatgga
    61261 tattggtggc ggtgaaatcg ctgttggcgg cattaggtga gaaacgccac actttgcgtg
    61321 agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata atggcgatac
    61381 cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc gcaagcgagc
    61441 caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgcagcttcc gggcgagtca
    61501 gactcacagt ccagacaatt tcgccctgat ggttcgcgcc aagttcaata actgcgtaat
    61561 cgtattccgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgttg ttgagattgc
    61621 ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc tctttaacgg
    61681 aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc cggaacttgc tggcgaaccc
    61741 atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc ggcaggtcga
    61801 tatccagcgg acggctaacc agtagtgcgc ctgcgccgcc agctttcgcc tggtcggcaa
    61861 aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa caggcagccc ggcgtcagtt
    61921 ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt tcaccgttga
    61981 gaatgtcggt aagttggcta agggttacgc taatcatgca atcaccccca gcagacgcgc
    62041 caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt aatcttcatg
    62101 gcctttgccc gcgaccagca ccacatcgtt ctctttagcc tgcataacgg cgcaagtcac
    62161 cgcttcagca cggtcttcca tcactttggc atgtccggca tctaacattc ccgccagaat
    62221 atcgttgatg atggcacgcg gctcttcggt acgcggatta tcgtccgtca ccaccgccac
    62281 atcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat cgcgatcgcc
    62341 accacagcca aagacacacc acagcttgcc cgcacagtgc agacgcgccg cctgtaaggc
    62401 tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt tgcctggcgc
    62461 agtgaacact tccatacgtc cgcaaaccgg ttgcagacga gcagcggttt tcagcagatc
    62521 agccagtgga tagcccagcg ccagcagcgt cgccagcgcg agcagcaggt tgctgacgtt
    62581 aaaagcgccc atcagatggc tttcaatttc gccatcgccc caacttgagc taaagcgaat
    62641 agtcgcaccg ctgtcgtgat agttcacttc ggtcgctttc aaccagcgtc cgtgacagtt
    62701 cggattgata tgatcttcca ttgataccgc aactgcatcc ggcagttttg ccagccagcg
    62761 gcggcctact tcatcgtcgg cattaataat cgcctgaccg caatgatgtt cagaataaag
    62821 cagccatttc gcagcttcgt agtgttccat atcaccatga taatcaaggt gatcgcggct
    62881 taagttagta aagaccgacg ccgcaaattt caatgccgcc acacggtgct gtaccagccc
    62941 gtgggaggaa acttccattg cgcaaaaagt cgcgtcctga tccaccagcc ccgccagctc
    63001 atgctgaaca tcaactgccg aaccggtggt attttctgtc gggatcactt tccccagcag
    63061 accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc tccactgcgc
    63121 cagcagctgg gtcgtcgtgg ttttgccgtt ggtgcccgtt acgcccacga gacgtaaatt
    63181 gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt tgagctggct
    63241 gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg cctcaccttt
    63301 cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat atcgacgccc
    63361 gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca cacggctgtc
    63421 gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag caagaaggtc
    63481 gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat tcatttttat
    63541 cgcccgttgt cagcgcatcc ggctcgatgt tcatggttcg caatacgccg cccatgatgg
    63601 caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc ggatcgttga
    63661 taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacacctgcg gtgtaagcaa
    63721 tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg gttttaatgg
    63781 caatacgata gcctttaatc gccgccttca cgccgccgcc acccggtagc gccacgcttt
    63841 ccatcatatg caccacagtg cggacaatag attccgggaa gacacgttca ccgggaaccg
    63901 gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg atagttgcgt
    63961 agactcgcgc taactgtaac ggagttacca ttagcccgta gccgaaagag aaggtggccc
    64021 tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct ccgaccaacc
    64081 ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac gctgaggacg
    64141 gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat actccggtca
    64201 gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt cgataaggaa
    64261 tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc atcaccacca
    64321 tcggtttaac cgttgaaccc ggttcaaaca cgtcggtgat ggtacggtta cgcatcgcct
    64381 ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc atcgccagca
    64441 cttcaccggt gttgacatcc accagcacgg cgctaccgga ctcagccttg ttaaaggcca
    64501 ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata ctcagcgcca
    64561 ggttgtgcgc agcctggctg tcagtagaag aaatatcttc aattacgcga ccatagcggt
    64621 ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa ctcttctcaa
    64681 cgccctcaat accttgacta tcgacgttag taaagccgat gaggtgagca gtcacttcgc
    64741 cggatggata ataacggcga gattcttcac gcagatgaat tcccggcagt ttcagttttt
    64801 tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag cgccctttcg
    64861 ggttggcgtt aatgcgggca gaaagctgat ccagcggaat attgagcgcg ttagccagcg
    64921 ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc gggtcagccc
    64981 aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca gtaatcatgc
    65041 cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct ttcaccagca
    65101 tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc agcgccagga
    65161 gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt tcttcctgac
    65221 gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct tatttttgca
    65281 ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc gtggcgatcc
    65341 tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg cgccattcaa
    65401 tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt aaacgggtat
    65461 ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac aggcagagtg
    65521 gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca tggcgctcgt
    65581 ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg atcatgcgtt
    65641 cgtcctctct gcaatacgca gaactgaact acgggcacga ggattctcag ccacctcttc
    65701 ttcgcccggc attaatttgc ctagtgctcg cagctgacgg ccacccagtt ttttgagctg
    65761 ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt cacgcataaa
    65821 acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc gcccacccgg
    65881 ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt cactgtttac
    65941 ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat ctttcaccgg
    66001 cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct gttcgcggtt
    66061 acgctcgaca atggctcggg caatgcgttt ggcaaaacgc tcttcaccat aggttttcaa
    66121 tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg actgcccacg
    66181 ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc cgcgttcagc
    66241 atcatcaagt tgcggtgaag agacaccaag atcgaggaga atgccgtcga tcttgccgat
    66301 aagatcgcgc tcggcaacgt attcgcccag cgcggaaaaa ggtccgtgga tgatggagaa
    66361 gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat cgatcgccag
    66421 caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac cgcgaccaaa
    66481 agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg cttcatccag
    66541 cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc tgcagtcgct
    66601 ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc tgttgatgcc
    66661 aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc acttcttttg
    66721 tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg ccatccatct
    66781 gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc gggttcatgc
    66841 tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg taaagcagca
    66901 ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc tcaagcagct
    66961 gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg actaacgttg
    67021 ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat ttcccactga
    67081 aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat accgaggtaa
    67141 aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac gtgcgaagca
    67201 aattaacgtt atgaatagct gtaagaaaaa aaccctcatc gtcatcaccg ttttaaaatg
    67261 actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg gcattttagg
    67321 acatatcttc cccggttaac agtccctgtt gcgtctgtgt agcgcttgat gccagcgggt
    67381 ttgcgcaaaa gcaaagaaaa gtgcttgtta aatcggcaat tcatctctgc atgtaacaaa
    67441 ataatgcaaa aacgcgctgt cgatatcact taattattgt gaatttcagc tggtgagtta
    67501 attaactggc gggtttaaaa gcatttatca ataatagtca ggaatagtct tatttacttt
    67561 aaggatattg atgtccagtc ccgtactcta cgcgccagag tgaaattcac ctggcgcgta
    67621 tttttgttcg cagcttagct acggctgagc acaccgcggc gatagagatt acgtttaatg
    67681 cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc
    67741 acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca ctgtaagaag
    67801 tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttaccatcg
    67861 cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt gaacagcgcc
    67921 tgcggcatcg gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca
    67981 tagctgttgg catacaggaa atgcacttcg cgcggatcat ctttccaggc agtacggaaa
    68041 ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc
    68101 tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca
    68161 accacgctgg taaagtgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg
    68221 tcgttagccc agcgttgata aaaaggatgc tcaggaggca acgacgtcga aacaataatg
    68281 gcatcgacct gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct
    68341 tctgagcagg caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta
    68401 gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg
    68461 cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca
    68521 gccatgactt tttcaacggt tttgtcactc acacggtatt gcttcgcttt gccgttaata
    68581 acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca
    68641 attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat
    68701 taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat
    68761 ttcgcaaaaa aagcccaacg tgacatgttg ggctgtaaat tgcgcattga gatcattctc
    68821 aacgcattat tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa
    68881 ttttcgccac atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta
    68941 attgaacggt ataaagcgag ggtgtgacat caataatttg cccacggaat atttccgtat
    69001 tacgtttaac ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc
    69061 tctcaacatg cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtata
    69121 attgcttttc gatctgctca agcacttttt catcgcccac ggtctggatg gtcatacgcg
    69181 ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag ccacgctggg
    69241 aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata
    69301 tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc
    69361 gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtgacatcaa caaataccag
    69421 gcgattatta cgcacctgtt caagcgcctc gctaagtttg ctttccagct cttgcggatg
    69481 agagatctgg atcccgacgt gaccataggc ttccgccaga cggacgaagt ctggtaaaga
    69541 ctgcatagac ggacgaagtc tggtaaagac tgcatataag attgtgaatg acgaccggaa
    69601 tagatcatgt cctgccactg cttcaccatc cccaaatagc ggttattaag attaaccacc
    69661 agcaccggca gttcatattg caacgctgta gacagttcct gaatgttcat ctgaatactg
    69721 ccgtcgccag tgacgcaaac cacggtttct tctggcagcg ccattttgac gcccagtgcc
    69781 gcaggtaaac caaagcccat agtgccgagg ccaccggagt tgatccaacg acggggtttg
    69841 tcgaatggat aataaagcgc agcaaacatc tggtgctgcc cgacatcgga cgtaacgtaa
    69901 gcgcccccct tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca
    69961 ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatctg ctgccaccag
    70021 tcgcggatct catccagcgg ttgatgggcg gattcttgcg acaagagttc aagcatttgt
    70081 tcgaggacct ggcgagcatc ccccacaatc ggaatatccg cagtcacggt tttagaaatg
    70141 gaagtaggat caatatcgat atgcagaaca gtggcatttg ggcagtactt tgccagattg
    70201 ttagtggtgc gatcgtcaaa gcggacaccg acagcaaaaa tcacatccgc gttatgcatc
    70261 gtcatattgg cttcgtaggt accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt
    70321 gccggaaacg ctcccagccc catcaatgag gaaacaacgg gcagatttaa cgcctccacc
    70381 gtttctttca gctgttgatg gcagcccgcc gtgattgccc caccgcctac gtagacaacc
    70441 ggtttttttg ccgccaccag cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta
    70501 gtagtgggat tgtaagaacg catactgacc gactccggcc agacataggg taatttgttc
    70561 gccggattaa gaatatcttt cggtaaatca acgaccactg gtcctgggcg accacttgcc
    70621 gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa
    70681 ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa
    70741 cctatcaacg aggtcgctac ctgcccggaa aggacaacta atggaatgga atccatataa
    70801 gcggtggcga tgccggtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg
    70861 ccgacttccc cggtcgcgcg cgccagacca tcggccatat gcactgccgc ctgctcatga
    70921 cgaactaaaa catgaccgat accacccacg gtatgcaatg catcgtaaat atcaaggact
    70981 gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc
    71041 atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta
    71101 aaacacatta accgtccgat cttgcttaag caattggcag ccagagacat aaacgccggg
    71161 taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacgaagga
    71221 taaaaatgat aataaatcgg ctaaatccca ccacttacag caatttctct atttttttaa
    71281 ttaatcctaa aatagagagc ccacacattc agaataaaat tctacactca ataaaaaatg
    71341 cagaataaac cagacattca tgtctgacct attctgcatt cagttagcgt ttgcaaattg
    71401 agactaattg ctcttccatc cactgatggc ctttatcacg cccggcagct tcatgccagg
    71461 agagataaca ggttctgcta tttagtttta acggcagcgg taatacctgt aattctaagg
    71521 attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca
    71581 ccacgctaag tacgctcatc attgccatgc cctgatacgc gatactggct tgcttatcta
    71641 ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt
    71701 gttcgttata aacatcatgt ttcagtaacg ggcccttaat tgttgggtga tttttgctgg
    71761 ctaccagcac catttcatct ttaaataatg gcacgctggt aaattcagga cgatggaagt
    71821 cttcataact aatcacaaac tccgtttcct gataacgcag ctgatgttca gtgttctgat
    71881 ttaatgaaga cttgaacata acatgtatat ttggcgcaat ctgctcaatg tgattataaa
    71941 tctgcgaggt cagaatactg tctaacgggc tgcaaacaca aagatgaaat acacgttcac
    72001 tgctcgcggg ttcaaaacct gaaccaggca attcattttg tactagttgc aatgcctgac
    72061 gaactgaacc aaaaagttga aatgcgcgag cagtcggttg aataccacgg ccataacgaa
    72121 caaaaagctc gtcattaaac atcaccttca ggcgtgcaac agcgttactg accgcaggtt
    72181 gcgacattcc cagaacatga gcagcacggg taatgttttg ctcctgcatc acggcatcga
    72241 aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgctgaac tccgccgtct
    72301 ctggatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt aact
//
LOCUS       DBFIFS010000004        65496 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.004, whole genome shotgun sequence.
ACCESSION   DBFIFS010000004 DBFIFS010000000
VERSION     DBFIFS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.004"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(181..663)
                     /gene="folK"
                     /locus_tag="N7A07_000222"
     CDS             complement(181..663)
                     /gene="folK"
                     /locus_tag="N7A07_000222"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU2474972.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            complement(660..2057)
                     /gene="pcnB"
                     /locus_tag="N7A07_000223"
     CDS             complement(660..2057)
                     /gene="pcnB"
                     /locus_tag="N7A07_000223"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU2474973.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2117..3013)
                     /gene="gluQRS"
                     /locus_tag="N7A07_000224"
     CDS             complement(2117..3013)
                     /gene="gluQRS"
                     /locus_tag="N7A07_000224"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU2474974.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3080..3535)
                     /gene="dksA"
                     /locus_tag="N7A07_000225"
     CDS             complement(3080..3535)
                     /gene="dksA"
                     /locus_tag="N7A07_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU2474975.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3713..4417)
                     /gene="sfsA"
                     /locus_tag="N7A07_000226"
     CDS             complement(3713..4417)
                     /gene="sfsA"
                     /locus_tag="N7A07_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU2474976.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4432..4962)
                     /gene="thpR"
                     /locus_tag="N7A07_000227"
     CDS             complement(4432..4962)
                     /gene="thpR"
                     /locus_tag="N7A07_000227"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU2474977.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            5036..7465
                     /gene="hrpB"
                     /locus_tag="N7A07_000228"
     CDS             5036..7465
                     /gene="hrpB"
                     /locus_tag="N7A07_000228"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU2474978.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7661..10195
                     /gene="mrcB"
                     /locus_tag="N7A07_000229"
     CDS             7661..10195
                     /gene="mrcB"
                     /locus_tag="N7A07_000229"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU2474979.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10415..12658
                     /gene="fhuA"
                     /locus_tag="N7A07_000230"
     CDS             10415..12658
                     /gene="fhuA"
                     /locus_tag="N7A07_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU2474980.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12709..13506
                     /gene="fhuC"
                     /locus_tag="N7A07_000231"
     CDS             12709..13506
                     /gene="fhuC"
                     /locus_tag="N7A07_000231"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU2474981.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13506..14396
                     /gene="fhuD"
                     /locus_tag="N7A07_000232"
     CDS             13506..14396
                     /gene="fhuD"
                     /locus_tag="N7A07_000232"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU2474982.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14393..16375
                     /gene="fhuB"
                     /locus_tag="N7A07_000233"
     CDS             14393..16375
                     /gene="fhuB"
                     /locus_tag="N7A07_000233"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU2474983.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16410..17690)
                     /gene="hemL"
                     /locus_tag="N7A07_000234"
     CDS             complement(16410..17690)
                     /gene="hemL"
                     /locus_tag="N7A07_000234"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU2474984.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17856..17903
                     /gene="yadX"
                     /locus_tag="N7A07_000235"
     CDS             17856..17903
                     /gene="yadX"
                     /locus_tag="N7A07_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU2474985.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17915..19336
                     /gene="clcA"
                     /locus_tag="N7A07_000236"
     CDS             17915..19336
                     /gene="clcA"
                     /locus_tag="N7A07_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU2474986.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19360..19425
                     /gene="yadW"
                     /locus_tag="N7A07_000237"
     CDS             19360..19425
                     /gene="yadW"
                     /locus_tag="N7A07_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU2474987.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19418..19762
                     /gene="erpA"
                     /locus_tag="N7A07_000238"
     CDS             19418..19762
                     /gene="erpA"
                     /locus_tag="N7A07_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU2474988.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19809..20432)
                     /gene="yadS"
                     /locus_tag="N7A07_000239"
     CDS             complement(19809..20432)
                     /gene="yadS"
                     /locus_tag="N7A07_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU2474989.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20470..21270)
                     /gene="btuF"
                     /locus_tag="N7A07_000240"
     CDS             complement(20470..21270)
                     /gene="btuF"
                     /locus_tag="N7A07_000240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU2474990.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21263..21961)
                     /gene="mtnN"
                     /locus_tag="N7A07_000241"
     CDS             complement(21263..21961)
                     /gene="mtnN"
                     /locus_tag="N7A07_000241"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU2474991.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22045..23562
                     /gene="dgt"
                     /locus_tag="N7A07_000242"
     CDS             22045..23562
                     /gene="dgt"
                     /locus_tag="N7A07_000242"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU2474992.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23692..25116
                     /gene="degP"
                     /locus_tag="N7A07_000243"
     CDS             23692..25116
                     /gene="degP"
                     /locus_tag="N7A07_000243"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU2474993.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKLISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25271..26428
                     /gene="cdaR"
                     /locus_tag="N7A07_000244"
     CDS             25271..26428
                     /gene="cdaR"
                     /locus_tag="N7A07_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU2474994.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26517..26903)
                     /gene="yaeH"
                     /locus_tag="N7A07_000245"
     CDS             complement(26517..26903)
                     /gene="yaeH"
                     /locus_tag="N7A07_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU2474995.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27218..28042)
                     /gene="dapD"
                     /locus_tag="N7A07_000246"
     CDS             complement(27218..28042)
                     /gene="dapD"
                     /locus_tag="N7A07_000246"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU2474996.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28073..30745)
                     /gene="glnD"
                     /locus_tag="N7A07_000247"
     CDS             complement(28073..30745)
                     /gene="glnD"
                     /locus_tag="N7A07_000247"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU2474997.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30807..31601)
                     /gene="map"
                     /locus_tag="N7A07_000248"
     CDS             complement(30807..31601)
                     /gene="map"
                     /locus_tag="N7A07_000248"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU2474998.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31969..32694
                     /gene="rpsB"
                     /locus_tag="N7A07_000249"
     CDS             31969..32694
                     /gene="rpsB"
                     /locus_tag="N7A07_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU2474999.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32952..33803
                     /gene="tsf"
                     /locus_tag="N7A07_000250"
     CDS             32952..33803
                     /gene="tsf"
                     /locus_tag="N7A07_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU2475000.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33950..34675
                     /gene="pyrH"
                     /locus_tag="N7A07_000251"
     CDS             33950..34675
                     /gene="pyrH"
                     /locus_tag="N7A07_000251"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU2475001.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34825..35382
                     /gene="frr"
                     /locus_tag="N7A07_000252"
     CDS             34825..35382
                     /gene="frr"
                     /locus_tag="N7A07_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU2475002.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35474..36670
                     /gene="ispC"
                     /locus_tag="N7A07_000253"
     CDS             35474..36670
                     /gene="ispC"
                     /locus_tag="N7A07_000253"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU2475003.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36859..37617
                     /gene="ispU"
                     /locus_tag="N7A07_000254"
                     /gene_synonym="uppS"
     CDS             36859..37617
                     /gene="ispU"
                     /locus_tag="N7A07_000254"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU2475004.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37630..38487
                     /gene="cdsA"
                     /locus_tag="N7A07_000255"
     CDS             37630..38487
                     /gene="cdsA"
                     /locus_tag="N7A07_000255"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU2475005.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38499..39851
                     /gene="rseP"
                     /locus_tag="N7A07_000256"
     CDS             38499..39851
                     /gene="rseP"
                     /locus_tag="N7A07_000256"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU2475006.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39881..42313
                     /gene="bamA"
                     /locus_tag="N7A07_000257"
     CDS             39881..42313
                     /gene="bamA"
                     /locus_tag="N7A07_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU2475007.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42434..42919
                     /gene="skp"
                     /locus_tag="N7A07_000258"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42434..42919
                     /gene="skp"
                     /locus_tag="N7A07_000258"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU2475008.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42923..43948
                     /gene="lpxD"
                     /locus_tag="N7A07_000259"
     CDS             42923..43948
                     /gene="lpxD"
                     /locus_tag="N7A07_000259"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU2475009.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44053..44508
                     /gene="fabZ"
                     /locus_tag="N7A07_000260"
     CDS             44053..44508
                     /gene="fabZ"
                     /locus_tag="N7A07_000260"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU2475010.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44512..45300
                     /gene="lpxA"
                     /locus_tag="N7A07_000261"
     CDS             44512..45300
                     /gene="lpxA"
                     /locus_tag="N7A07_000261"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU2475011.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45300..46448
                     /gene="lpxB"
                     /locus_tag="N7A07_000262"
     CDS             45300..46448
                     /gene="lpxB"
                     /locus_tag="N7A07_000262"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU2475012.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46445..47041
                     /gene="rnhB"
                     /locus_tag="N7A07_000263"
     CDS             46445..47041
                     /gene="rnhB"
                     /locus_tag="N7A07_000263"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU2475013.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47078..50560
                     /gene="dnaE"
                     /locus_tag="N7A07_000264"
     CDS             47078..50560
                     /gene="dnaE"
                     /locus_tag="N7A07_000264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU2475014.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50573..51532
                     /gene="accA"
                     /locus_tag="N7A07_000265"
     CDS             50573..51532
                     /gene="accA"
                     /locus_tag="N7A07_000265"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU2475015.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51631..53772
                     /gene="ldcC"
                     /locus_tag="N7A07_000266"
     CDS             51631..53772
                     /gene="ldcC"
                     /locus_tag="N7A07_000266"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU2475016.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53829..54218
                     /gene="yaeR"
                     /locus_tag="N7A07_000267"
     CDS             53829..54218
                     /gene="yaeR"
                     /locus_tag="N7A07_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2475017.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54283..55578
                     /gene="tilS"
                     /locus_tag="N7A07_000268"
     CDS             54283..55578
                     /gene="tilS"
                     /locus_tag="N7A07_000268"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU2475018.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55631..55891)
                     /gene="rof"
                     /locus_tag="N7A07_000269"
     CDS             complement(55631..55891)
                     /gene="rof"
                     /locus_tag="N7A07_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU2475019.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55878..56078)
                     /gene="yaeP"
                     /locus_tag="N7A07_000270"
     CDS             complement(55878..56078)
                     /gene="yaeP"
                     /locus_tag="N7A07_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU2475020.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56244..56789
                     /gene="yaeQ"
                     /locus_tag="N7A07_000271"
     CDS             56244..56789
                     /gene="yaeQ"
                     /locus_tag="N7A07_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU2475021.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56786..57196
                     /gene="arfB"
                     /locus_tag="N7A07_000272"
     CDS             56786..57196
                     /gene="arfB"
                     /locus_tag="N7A07_000272"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU2475022.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57210..57920
                     /gene="nlpE"
                     /locus_tag="N7A07_000273"
                     /gene_synonym="cutF"
     CDS             57210..57920
                     /gene="nlpE"
                     /locus_tag="N7A07_000273"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU2475023.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58120..58944)
                     /gene="yaeF"
                     /locus_tag="N7A07_000274"
     CDS             complement(58120..58944)
                     /gene="yaeF"
                     /locus_tag="N7A07_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGU2475024.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58997..60715)
                     /gene="proS"
                     /locus_tag="N7A07_000275"
     CDS             complement(58997..60715)
                     /gene="proS"
                     /locus_tag="N7A07_000275"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU2475025.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60826..61533)
                     /gene="tsaA"
                     /locus_tag="N7A07_000276"
     CDS             complement(60826..61533)
                     /gene="tsaA"
                     /locus_tag="N7A07_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU2475026.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61530..61934)
                     /gene="rcsF"
                     /locus_tag="N7A07_000277"
     CDS             complement(61530..61934)
                     /gene="rcsF"
                     /locus_tag="N7A07_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU2475027.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(62052..62867)
                     /gene="metQ"
                     /locus_tag="N7A07_000278"
     CDS             complement(62052..62867)
                     /gene="metQ"
                     /locus_tag="N7A07_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU2475028.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62907..63560)
                     /gene="metI"
                     /locus_tag="N7A07_000279"
     CDS             complement(62907..63560)
                     /gene="metI"
                     /locus_tag="N7A07_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU2475029.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63553..64584)
                     /gene="metN"
                     /locus_tag="N7A07_000280"
     CDS             complement(63553..64584)
                     /gene="metN"
                     /locus_tag="N7A07_000280"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU2475030.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64772..65347
                     /gene="gmhB"
                     /locus_tag="N7A07_000281"
     CDS             64772..65347
                     /gene="gmhB"
                     /locus_tag="N7A07_000281"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU2475031.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15462 a        16265 c        18070 g        15699 t
ORIGIN      
        1 atatatttac gtggtgtatt gaattgttgc tattctaggt tttttaaaca aaaaatgcct
       61 aatctatttg catgaagtgc tatttcatga attaattaaa aaaaactaaa atgtaaatta
      121 agtatattta atctgtaaga ggggaagtga ggagtaagat aagaaaataa aataatacag
      181 ctaataccag atggcaggtt gtagtactgc tagcgaagtg agtatttcac gtaatgtctc
      241 cccatcagga aacacgagtt ccggggcaat ctcaaacagc ggccacagca taaagccgcg
      301 attcttcata tcgtaatgcg ggacggtgag tcgttcggta ttaatggttt cattaccaaa
      361 caacatgata tcaagatcga gtgtgcgcgg tccccagcgt tctgctttac gaacgcgacc
      421 ttgctgcaac tctatttgct gtgtgtgatt aagtaattct tcaggcgcaa gtgttgtttc
      481 cagcgccacg gctgcgttta agtaatcggg ttgatcttgc ggccccagcg gtggggtgcg
      541 gtaaaacgaa gaaacggtaa gaatgtggct ttcagggata tcgcctaatg ctttcagggc
      601 agcattgacc tgctccagcg gagaggccag attgctgcct atggcaatat acgccactgt
      661 catgcggtac cctcacgacg tggtgcgcgt ttgcgtggac gacgagtacg acgacgcggt
      721 gacggttctt catccagctc gttgagcatc cctttttggt ctggtggcgc ggaaacctgg
      781 aactcacccc accatttcac cagacgctgc agttcagcgt tacgctcaac ttcagctcgc
      841 aaggccaaca ggtcataagc cgcacggaac ttaggatgct ccagcaattt ccatgcgcgt
      901 ttaccctgac gtcgggacat acgcaactgc aactgccaga tatcgcgggt taacgtcgtc
      961 agacgtttcg ggattgccag tgaacggcag gcttcgtcca gcacgtcgtt catcgccagc
     1021 gcgaaagcgt cgtgataggt caggccgctt tcctgggcga tcttctgtgc cgtctccagc
     1081 agtgggtacc agaacatggc ggcaaacagg aacgccgggt tcacgcgcat atcgttatgg
     1141 atacgcgtat cggtattctt cagcacctgt tcaatgatcc gctccatcgg gctgtcgcca
     1201 ttttccgtga agtagcgggt aatggtcggg aacagcggct ggaacagatg atattcacac
     1261 aacagcttat aggtttcgta accgtagccc gcttgtagca gtttaagcga ttcttcaaac
     1321 aggcgtgccg gtgggatatc gttcagcagg gtagcgaggc gagggatcgg ttctgcggtt
     1381 tccgggctga tgcgcatacc caatttggcg gcaaaacgta ctgcgcgcag catacgtacc
     1441 ggatcttcac ggtagcgcgt ttccgggtta ccaatcagac ggataacacc gtccttcaga
     1501 tccttcatac cgccaacgta atcacggacg gtaaaatccg ctacgctgta atacaggctg
     1561 ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat gttgtcgcgc
     1621 agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt accttcgtgg
     1681 tgtccacgga aggtcgcaac ttcgataatc tccgggccaa acatcacatg agccagacgg
     1741 aaacggcgac ccaccaggcg gcagttacgg aacagtttgc gcacctgctc aggcgtggcg
     1801 ttagtggtta cgtcaaaatc tttcggcttt ttgccgagta acaggtcgcg cacgccgccg
     1861 ccaaccagcc aggcttcgta tcccgcttta ttgagcctgt acattacctt cagggcattt
     1921 tcactgatat ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt cacctgtgga
     1981 cgggcgactg cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg gcaaaaatta
     2041 gcgactcggg taaaaatagt acacctcggt agtgtcaaac atcattcagg acaaaaaaat
     2101 agcggctaat catagctcag catgacgcat ttgagaatgt tgaatttaca attgccgact
     2161 caggcactgc ggtaagccgc cagtttttga cggctgactg aaggatttgc tcgacgctga
     2221 aatcctgcca gtgtgcttct gcctgctgcc ccagaaattg aagtgccgcg attagtaccg
     2281 ggcgtggatc gcctttcggc aacgcaggcg catgattctg cttggaaagt ttagcgcctt
     2341 gtggattaag cgccagcggc agatgaatgt aatctggcac tttccagcca aaaagctggt
     2401 acagcgagat ttgccgcact gtcggttcga tcaaatctgc cccgcggaca atttcgctca
     2461 cgccctggaa gtgatcatcc accaccaccg ccaggttata ggcaaacaaa ccatcacggc
     2521 gatgaataat aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg ccgcgcagct
     2581 ggtcagtaaa ttgcgtgacc ggatgctgct ggcggatacg cactgcggcg ttgtctggtc
     2641 catgatgcaa cacccggcaa tgaccgtcgt aaataccgcc aatgctttga atacgcgcac
     2701 gcgtgcaggt gcagtaataa cttagtcctt gttcatgtaa ccaggcgagt gcttcacgat
     2761 aggcgtggtg acgttgcgat tgccagagaa catcgccgtc ccagtgcaga ccgtaatgtt
     2821 ccagctggcg caggatagtt tctgcggcac cgggaacttc acgaggcggg tcgatatctt
     2881 caatgcgtac cagccagcga ccgtgccggg cgcgagcctg caaatagctg ccaagcgcag
     2941 caatcagaga accaaaatga agctcgccgg aaggagaggg ggcgaagcgg ccaatatact
     3001 gtgtgtctgt catctctttg aacaaaaaat aaggcgggag catttcccgc ctgtggtaaa
     3061 cgtgatggaa cggctgtaat tagccagcca tctgtttttc gcgaatttca gccagcgttt
     3121 tgcagtcgat gcacagatcg gctgtcgggc gcgcttccag acggcgaata ccaatttcaa
     3181 caccgcagga ttcacagtag ccgaaatctt cgtcttccac ttttttcagc gtcttctcga
     3241 tctttttgat cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg aactcttctt
     3301 cctgggctgc acggtctacc gggtccggga aattggctgc ttcatcctgc atatgtgtaa
     3361 cggtgcgatc gacttcatcc ctgagttgat tacgccatgc ttccagaata cgacggaagt
     3421 gcgccagctg ggcttcattc atatactctt cgcccggctt ctcctgatat ggttccaccc
     3481 cagcgatggc gagaatactc agggacgatg ttttacggtt ttgcccttct tgcatgttgc
     3541 ttctccttaa cacgcactat cgatccccat gttcggggga aaaatgaggc cgctataaat
     3601 agcagatgct tttccggata gcaattatct aaacgtaaca cttgacaact gtgtgaggaa
     3661 aagcgtattt gcgcacgcga ccagaatgta aattaaccag ttacttactt tactacaatg
     3721 taaccggcag tgatttttta agagccatgc cttcagcaga aatttccgct ttgtaagcca
     3781 gaatttctac ccccctctgt tgagcttctg acaatagttg cgcgtatttc tcatcgatgt
     3841 ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag ataaccgcac
     3901 gctggccttc agccgctacg ctcatcaact cccgaaggtg tttctgacct cgttcagtga
     3961 ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt aaccgatttc acttcaatat
     4021 agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt tctgcgccgt
     4081 attttacttc gcttttcagc gagctatagc ctgacagttc tgaaattgat tcattaagga
     4141 tagcctcttt cgtcaacctg ttagcccaaa gcgtgttgac gcaaataaat gcgccgctct
     4201 ggctttgagt taattcccag gtgtgtgggt atttccgttt ggtgttgtct gaagtcgaat
     4261 accagacggt atcgccaggc gttgcacaac cggtcatcgc acccgtattc gggcagtgta
     4321 gcgttaattc gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg taacgctgaa
     4381 ttagcgtcgc gcgctgtaga gggggagaaa attccattgc gacaatcctt gttattgcgt
     4441 tagcacccag cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg aggaggcgta
     4501 aagggtgaac tccgtcaccg catatgacca gttaaaacct ggcggcggga ttgtcaccgc
     4561 ctcgctggcg tcgcgcaata aggtaatatg tggatgaaac ggacgattgc tttgaaaaca
     4621 accgctgcgg gcagcctgtg aacggagcat attcgccagc tggattaagc cgcgtggcgg
     4681 ctgacgcatc cctaaccaca ccacacgcga acgcagccat tggccggcgt catcaagcgt
     4741 gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct tctctttctc
     4801 tgcgctcact tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg cgaccggacg
     4861 tcccgcctca agtgggaagt gtgtggcgcg ccagtggata atctgttcgc ggatttctgc
     4921 aggtaagtcg atagcaaaga acagacgttg cgggtcagac atgtgaggca ctcggttatg
     4981 aattaccgcg atgctacaat gtggcgcgaa gaatgttaac cctctggagc gttttgtgtc
     5041 gtcgttgccc gttgctgccg tgttacctga attacttacc gctctcgatt atgcgccgca
     5101 ggtattatta agtgcgccga ccggggccgg gaaatcaacc tggctgccgc tgcaactgct
     5161 ggcgcatccc ggcattaacg ggaaaattat cctcctggag ccacggcgtc tggcggcgcg
     5221 taacgtcgcg caacggctgg cggagctgct taacgaaaag ccaggcgata ccgttggcta
     5281 ccggatgcgt gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtag ttaccgaagg
     5341 cgtgctgacg cggatgatcc agcgtgaccc ggaactgagc ggtgttggat tggtgatcct
     5401 tgatgaattt catgagcgta gcttgcaggc ggatttggcg ttggcgctgt tactcgatgt
     5461 gcaacaaggt ctgcgtgatg accttaaact gctgattatg tcggctacgc tggacaacga
     5521 ccgcttgcag caaatgctgc cagaagcgcc cgtcgtcatc tcagaagggc gctcgtttcc
     5581 ggttgaacgc cgttatttac cgctgcccac gcatcagcgt tttgacgatg ccgttgcggt
     5641 agccaccgct gaaatgctgc gtcaggaaag cggatcatta ctgttatttt tacctggcgt
     5701 cggagaaatt cagcgtgtgc aggaacaact ggcttcgcgc atcggcagtg atgtattgct
     5761 ctgcccgctg tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc tcccggcacc
     5821 gcaagggatg cgcaaagtgg tgctggcgac caatattgct gaaaccagtt taaccattga
     5881 aggtattcgt ctggtggtgg attgtgccca ggaaagggtg gcgcgttttg atccgcgaac
     5941 agggcttacg cggttgatta ctcagcgaat cagtcaggcg tcgatgacgc agcgtgccgg
     6001 gcgcgccggg cgtctggagc cgggtatctg cctgcattta atcgccaaag aacaagcaga
     6061 acgcgctacc gcgcaaagtg aaccggagat cttacaaagc gatctttccg gtttgctgat
     6121 ggaattactg caatggggat gcagcgatcc ggcgcagatg agctggctgg atcaaccgcc
     6181 aacggtgaat ctactggccg cgaaacgtct gttacaaatg ctgggggcgc tggacggtga
     6241 acggcttagt gcgcaagggc aaaaaatggc agcgctgggt aacgatccgc gtttagcggc
     6301 aatgctggtt agcgcaaaga gcgacgacga agctgctacc gcggcaaaaa ttgccgccat
     6361 tctcgaagag ccgccacgga tgggcaatag tgacctgggc gtggcgtttt cgcgcaatca
     6421 accagcctgg cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac gcggcggtga
     6481 ggcagacagt tcgcttatcg cgccgctact tgccggggcg tttgccgatc gcattgctcg
     6541 ccgccgtggg caagatggac gctatcaact ggcaaacggc atgggggcga tgcttgatgc
     6601 cgacgacgcg ctaagtcgcc acgaatggtt gatcgcgccg ttattattgc agggcagcgc
     6661 ctcgccggat gcgcggattt tactggcgtt accggtcgat attgatgaat tagtacaacg
     6721 ctgtccgcag ctggtgcagc aatctgacac cgtggaatgg gatgacgcgc aaggtacgct
     6781 gaaagcctgg cgtcggctgc aaatcggtca gttgacggtg aaagtgcagc cgttggcgaa
     6841 accgtcagaa gacgagttgc atcaggcgat gcttaacggc atccgtgata aaggtttaag
     6901 cgtgctcaac tggacggcgg aagcggaaca gctacgcttg cgtttgttat gcgccggaaa
     6961 gtggttgcca gaatatgact ggccagcggt tgatgatgaa agtttgttgg cgacgctgga
     7021 aacgtggctg ctgccacata tggccggtgt acattcacta cgcggcctga aatcactcga
     7081 catttatcag gcactacgcg gattacttga ttggggaatg cagcaacgtc tggatagtga
     7141 attgcctgcg cattacactg tgccgacggg aagccggatc gccattcgtt atcatgaaga
     7201 taacccgccc gcgctggcgg tgagaatgca ggagatgttt ggtgaggcca ctaatccgac
     7261 gatcgcccag gggcgcgtgc cgctggtgct ggaattgctt tcacccgccc aaaggccgct
     7321 gcaaatcacg cgtgatttgg gggccttctg gaaaggggcg tatcgtgagg tgcaaaagga
     7381 gatgaaaggg cgttatccca aacacgtctg gccggacgac ccggcaaaca cggcaccgac
     7441 gcgacggacg aaaaagtatt cgtaaggttg gttttctccc tctccctgtg ggagagggcc
     7501 ggggtgaggg caccagcgcg cacgttcacc ctcaccctaa ccctctccct taagggagag
     7561 gggaccgatc gagctcaaat tttgagagat atcttcttct gtcttgtaac agaagaacag
     7621 aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc atggccggga atgaccgcga
     7681 gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa gccgtcgtcg
     7741 ttacgaagat gacgatgatt acgacgatta tgatgactat gaggatgaag aaccgatgcc
     7801 gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg gctggctatg
     7861 gctgctgtta aaactggcta tcgtttttgc cgtgctgatc gccatttacg gcgtttatct
     7921 cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg caactgcctg cggcagttta
     7981 tggtcgaatg gtcaaccttg agccagacat gaccatcagc aagaacgaga tggtgaagct
     8041 gctggaggca acacagtatc gtcaggtgtc gaaaatgacc cgtcctggcg aatttaccgt
     8101 gcaggccaac agcattgaga tgattcgccg tccgtttgat ttcccggaca gtaaagaagg
     8161 gcaggtgcgc gcgcgtctga cctttgatgg cgatcatctg gcgaccatcg tcaatatgga
     8221 gaacaaccgt cagttcggtt tcttccgtct tgatccgcgt ctgatcacca tgatctcctc
     8281 accaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt ttcccggatt tgctggtgga
     8341 tactttgctg gcgacagaag accgccattt ctacgagcat gatggaatca gtctctactc
     8401 catcggacgt gcggtgctgg caaacctgac cgcaggtcgc acggtgcagg gggcgagtac
     8461 gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgctctt attggcgtaa
     8521 agcgaatgaa gcctacatgg cgctgatcat ggacgcgcgt tacagcaaag accgtattct
     8581 tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa tccgcggctt
     8641 cccgctggcg agcttgtatt actttggtcg cccggtagaa gaattgagcc tcgatcagca
     8701 ggcgctgtta gtcggtatgg tgaaaggagc gtccatctac aacccgtggc gtaacccgaa
     8761 actggcgctg gagcgacgta atctggtact gcgtctgctg caacagcaac agattattga
     8821 tcaagaactc tatgacatgt tgagtgcgcg tccgctgggg gttcagccgc gcggtggggt
     8881 gatctctcct cagccagcct ttatgcaact ggtgcgtcag gagcttcagg ctaaattagg
     8941 cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact cggtggccca
     9001 ggacgcggca gaaaaagccg ccgtggaagg tattccggca ctgaagaaac agcgtaagtt
     9061 gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt agtggtgaag ttcgtgcgat
     9121 ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg cgcgtcgttc
     9181 gattggttcc cttgcaaaac cagcgactta tctgacggcc ttaagccagc cgaaaatcta
     9241 tcgtctgaat acgtggattg cggatgcgcc aattgcgctg cgtcagccga atggccaggt
     9301 ctggtcaccg cagaatgatg accgtcgtta tagcgaaagc ggcagagtga tgctggtgga
     9361 tgcgttgacc cgttcgatga acgtgccgac ggtaaatctg gggatggcgc tggggctgcc
     9421 tgcggttacg gatacctgga ttaaactggg cgtaccgaaa gatcagttgc atccggttcc
     9481 ggcaatgctg ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg cattccagac
     9541 catcgccagc ggtggtaacc gtgcaccgct ttctgcgctg cgttcggtaa tcgcggaaga
     9601 tggcaaagtg ctgtatcaga gcttcccgca ggcggaacgc gctgttccgg cgcaggcggc
     9661 gtatctgaca ctatggacca tgcagcaggt ggtacaacgc ggtacgggtc gtcagcttgg
     9721 ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca ataacgtaga
     9781 tacctggttt gcgggcattg acggcagcac ggtgaccatc acctgggtcg gccgtgataa
     9841 caaccagccg accaaactgt atggagccag cggggcaatg tcgatttatc agcgttatct
     9901 ggctaaccag acgccaacgc cgctgaatct tgttccgcca gaagatattg cagatatggg
     9961 cgtggactac gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc cggtctggac
    10021 cagcgatccg caatcgctgt gccagcagag tgagatgcag cagcagccgt caggcaatcc
    10081 gtttgatcag tcttctcagc cgcagcaaca gccgcaacag caacctgctc agcaagagca
    10141 gaaagacagc gacggtgtag ccggttggat taaggatatg tttggtagta attaacatct
    10201 aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa taacatccca cccaaagtat
    10261 taaccctttc ttttcatctg gttgtttatt aacccttcag gaacgctcag attgcgtacc
    10321 gcttgcgaac ccgccagagt ttcgaatatt atcttatctt tataataatc attctcgttt
    10381 acgttatcat tcactttaca tcagagatat accaatggcg cgttccaaaa ctgctcagcc
    10441 aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg gcatgtctgt
    10501 ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg ctgcacctgc
    10561 gccgcaagaa agcgcatggg ggcctgctgc aactattgcg gcgcgacagt cagctaccgg
    10621 cactaaaacc gatacgccga ttcaaaaagt gccacagtct atttctgttg tgaccgccga
    10681 agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg cttagctaca cgccgggtgt
    10741 ctctgttggt acgcgtggcg catccaacac ctatgaccac ctgatcattc gcggttttgc
    10801 ggcagaaggc caaagccaga ataactatct gaatggcctg aagttgcagg gcaacttcta
    10861 taacgatgcg gtcattgatc cgtatatgct ggaacgcgct gaaattatgc gtggcccggt
    10921 ttccgtgctt tacggtaaaa gcagtcctgg cggcctgttg aatatggtca gcaagcgtcc
    10981 gaccaccgaa ccgctgaaag aagttcagtt taaagccggt actgacagcc tgttccagac
    11041 tggttttgac tttagcgatg cgctggatga tgacggcgtt tactcttatc gcctgaccgg
    11101 tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa gagcagcgtt atgctattgc
    11161 accggcgttc acctggcgtc cggatgataa aaccaatttc accttccttt cttacttcca
    11221 gaacgagccg gaaaccggtt attacggctg gttgccgaaa gagggaaccg ttgagccgct
    11281 gccgaacggt aagcgtctgc cgacagactt taacgaaggg gcgaagaaca acacctattc
    11341 tcgtaatgag aagatggtgg gctacagctt cgatcacgaa tttaacgaca cctttactgt
    11401 gcgtcagaac ctgcgctttg ctgaaaacaa aacctcgcaa aacagcgttt atggttacgg
    11461 cgtctgctcc gatccggcga atgcttacag caaacagtgt gcggcattag cgccagcgga
    11521 taaaggccat tatctggcac gtaaatacgt cgttgatgat gagaagctgc aaaacttctc
    11581 cgttgatacc cagttgcaga gcaagtttgc cactggcgat atcgaccaca ccctgctgac
    11641 cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc tggtttggtt acgacgactc
    11701 cgtaccgctg ctcgatctgt acaatccggt gaataccgat ttcgacttca atgccaaaga
    11761 tccggcaaac tccggccctt accgcattct gaataagcag aaacaaacgg gcgtttatgt
    11821 tcaggatcag gcgcagtggg ataaagtgct ggtcaccctg ggcggtcgtt atgactgggc
    11881 agatcaagaa tctcttaacc gcgttgccgg gacgaccgat aaacgtgatg acaaacagtt
    11941 tacctggcgt ggtggtgtta actacctgtt tgataatggc gtaacacctt actttagcta
    12001 tagcgaatcg tttgaacctt cttcgcaagt tgggaaggat ggtaatattt tcgcaccgtc
    12061 taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg gaagatcgtc cgattgtagt
    12121 tactggtgcc gtgtataatc tcactaaaac caacaacctg atggcggacc ctgagggttc
    12181 cttcttctcg gttgaaggtg gcgagatccg cgcacgtggc gtagaaatcg aagcgaaagc
    12241 ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac
    12301 caccgatact acctataaag gcaatacgcc tgcacaggtg ccaaaacaca tggcttcgct
    12361 gtgggctgac tataccttct ttgacggtcc gctttcaggt ctgacgctgg gcaccggtgg
    12421 tcgttatact ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac
    12481 ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt
    12541 ggcgctgcat gttaacaacc tgttcgatcg tgaatacgtc gccagctgct ttaacactta
    12601 tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt
    12661 tctcttttgg ggcacggatt tccgtgcctg tttcacaagt tggctgttat gcaggaatac
    12721 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12781 acgcttttgc atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt
    12841 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gccgtcatca gccgccgtcg
    12901 gaaggggaga ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgttcgcc
    12961 cgcaaagtgg cttatttgcc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    13021 ctggtggcga ttggtcgtta cccgtggcat ggcgcactag ggcgctttgg tgcggcagat
    13081 cgcgaaaaag ttgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    13141 gtagacagcc tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    13201 gatagccgtt gtctgttgct cgacgaaccg acttcggcgt tggatatcgc ccaccaggtt
    13261 gatgtgctgg cgctggtgca ccgtttaagt caggagcgtg gcctgacggt cattgccgtg
    13321 ctgcacgata tcaatatggc tgcgcgttac tgtgattatc tggtcgccct gcgcggcggt
    13381 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt
    13441 tatggcatcc cgatgggtat tctgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    13501 tattgatgag cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt
    13561 ctccgttgtt atggcagatg aataccgccc acgcggcggc tattgaccca aatcgtattg
    13621 tggcgctgga gtggctgccg gtggaattac tactggcgct cggcatcgtg ccttatggcg
    13681 tggcagacac catcaactat cgactgtggg tcagcgaacc accactacct gaatcggtga
    13741 ttgacgtcgg tttgcgtaca gaacctaacc tcgaactgct gaccgaaatg aaaccgtcgt
    13801 ttatggtctg gtcggcggga tatggtcctt cgccagaaat gctggctcgt attgcgccgg
    13861 ggcgtggatt taacttcagc gacggcaaac agccgctggc gatggcgcgt aaatcgctga
    13921 cggaaatggc ggatttactt aacctgcaaa gcgcagcaga aacgcattta gcgcaatatg
    13981 aagactttat ccgcagcatg aacccccgct ttgtgaagcg tggtgcgcgt ccgttattgc
    14041 tgacgacgct tatcgatccg cgccatatgc tggtcttcgg tccaaacagc ttgttccagg
    14101 aaattcttga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    14161 gtaccgccgt cagcatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    14221 atcacgacaa cagcaaagac atggatgcgc taatggcaac gccgctgtgg caggccatgc
    14281 cgtttgtccg cgccggacgc tttcagcgcg tacccgcagt ctggttttat ggtgcaactc
    14341 tctcggcaat gcactttgtg cgcgttctgg ataacgccat tggaggtaaa gcgtgagtaa
    14401 acgtattgcg cttttcccgg cattattgct ggcgttgctg gtgattgccg ctacggcgct
    14461 gacctggatg aacttctcgc aggcgctgcc gcgtagccag tgggcgcagg ctgcctggtc
    14521 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgctgc cgcgtctggc
    14581 gatttcgctg ctggtgggcg caggcctggg gctggtgggc gtgctgtttc agcaagtgct
    14641 gcgtaatccg ctggcggaac cgacgacact tggcgttgct acaggcgcgc aactggggat
    14701 taccgtcact acgctctggg cgatcccagg ggcgatggcg agtcagtttg ctgcgctggc
    14761 gggcgcttgc gttgttggct taatcgtctt tggcgtcgcg tgggggaaac ggctttcgcc
    14821 ggtaacgctg atcctcgcgg ggctggtagt gagcctttat tgtggggcaa tcaatcagtt
    14881 actggttatc ttccaccatg accaactgca aagcatgttc ctgtggagca ccggaacgct
    14941 gacgcaaacc gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat
    15001 gctgacgttg ctgctacttc gtccgttaac actgatgggg cttgatgatg gcgtggcgcg
    15061 caatctcggg ctagccttgt cgctggcgcg tctggcggcg ctgtcgctgg ctattgtcat
    15121 cagtgcgctg ctggtgaacg cggtggggat tatcggcttt atcgggttgt tcgcgccgtt
    15181 gctggcaaaa atgctggggg cgcggcgtct gctgccgcga ctgatgctgg cgtcgttgat
    15241 tggtgcgctg atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat
    15301 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgtt
    15361 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtc aacgatcgtg tcgcggctga
    15421 acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg ctgctgttga tggctgtggt
    15481 ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg tgggcgagcg gggcgttgct
    15541 cgaggatttg atgccctggc gctggccgcg aattatggcg gcgctgtttg cgggcgtcat
    15601 gctggcggtg gcgggctgta ttattcagcg gctgaccgga aacccgatgg caagcccgga
    15661 agtgctgggg attagctctg gcgcggcgtt tggcgtggta ctgatgctgt ttctggtacc
    15721 gggtaatgct tttggctggc tgttacccgc gggaagtatc ggtgcggcgg tgacgctgtt
    15781 gattattatg atcgccgccg ggcgcggtgg attttcccca caccgcatgt tactggcggg
    15841 gatggcgtta agtaccgcat tcaccatgct tttaatgatg ttgcaggcga gtggtgaccc
    15901 gcgaatggcg caagtgctga cctggatctc tggttcgacc tacaacgcca ccgatgcgca
    15961 ggtctggcgc accggaattg tgatggtgat tttgctggcg attaccccgc tgtgccgccg
    16021 ctggctgacc attttaccgc tgggtggtga taccgcgcgg gccgtggggg tggcgttaac
    16081 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac
    16141 cattggaccg ttgagttttg ttggtttaat ggcaccgcat atcgcgcgga tgatgggctt
    16201 tcgacggacg atgccgcaca tcgtgatttc ggcgctggtg ggtggtttac tgctggtgtt
    16261 cgctgactgg tgtgggcgga tggtgctgtt tccgttccag atcccggcgg ggctgctgtc
    16321 gacctttatc ggcgcgccat attttatcta tttgttgaga aagcagagcc gttaataaaa
    16381 tgcctcgccc ggtgggcgag gcaaaatcat cacaacttcg caaacacccg acgtgcagca
    16441 tcgatggtgt tattgatatc ttccatgctg tgcgccacag acataaagcc cgcttcaaac
    16501 gctgacggtg ccaggtaaac accttcatcc agcatcatat ggaagaaacg cttaaaacgc
    16561 tcgacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaaa
    16621 ataccgaaca tgccgccaac gtggtttacc accagcggaa ttccggcttc ttctgccgct
    16681 tccagcagac cttctgccag acgtgttgtc agctcatcca gcgtttcgtg aacgcccggc
    16741 tgcgcgactt cattcagaca ggcgaaaccg gctgccatcg caatcgggtt accggaaagc
    16801 gtacccgcct gatagaccgg acccgtcggg gccagcgcat ccattacatc acgacgacca
    16861 ccgaatgcgc ctaccggcat tccaccgccg atgattttgc ccaggcaagt gagatccggt
    16921 tccacaccgt aataatcctg tgcgccagct agcgctacgc ggaaaccggt cattacttca
    16981 tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcgc gcagacctgg caggaagtct
    17041 ggcagcggtg gaacacagtt catattgcct gccaccggct cgacgataat acaggcaatc
    17101 tcttgcgggt attgctcaaa cgcggcgcgt acagaagcca gatcgttata agtacaggtt
    17161 aaggtatgtt tggcgaaatc tgccggaacg cccggcgagt ttggctggcc taacgtgagt
    17221 gcgccagagc cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat
    17281 ttaataattt tgtcgcgacc ggtaaaacca cgggccaggc ggatggcgct cattgtcgct
    17341 tcagtgccgg agttcaccat gcgcaccata tccatggtcg ggaccagttc ggtcaccagt
    17401 tgcgccattt tcacttccat ttcggttggt gcaccaaagc ttaaaccacg ctcggcggct
    17461 tcaatcacgg cattgcggat cgccggatgg ttatggccca gcaccatcgg cccccaggaa
    17521 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    17581 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacaccg
    17641 ccagggatca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    17701 tgattcgtag aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc
    17761 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    17821 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    17881 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    17941 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    18001 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg tcgggctggc
    18061 agcggttgct tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt
    18121 acatactgct gataattatc cgctactgtt aaccgttgct tttctctgtt cggcggtgct
    18181 ggcgatgttc ggctactttt tggtgcgcaa atacgcgccc gaagcagggg ggtcggggat
    18241 cccggaaatt gaaggggcgc tggaagatca acgtcctgtt cgctggtggc gtgtattgcc
    18301 ggtgaaattc tttggcgggc tggggacact cggcggaggc atggtgttgg ggcgcgaagg
    18361 gccaaccgtg cagatcggcg gtaatatcgg gcggatggta ctcgatgttt tccgcctgaa
    18421 aggtgatgaa gctcgccata cgctgttggc aaccggtgcg gcggcgggtc tggcggcggc
    18481 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    18541 ctatacgtta atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta
    18601 ccggattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg acgcgccgct
    18661 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    18721 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggcg gcaatattac
    18781 caaatgggta ctgatgggtg gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    18841 accagcaacg tcgggcggcg gttttaacct gattcctatc gctaccgcgg ggaatttcag
    18901 catgggaatg ctggtgttta tcttcgtcgc gcgggtcatt accaccttac tctgcttctc
    18961 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    19021 cgctttcgga atggttgccg ttgagctgtt tccgcaatat caccttgagg cggggacgtt
    19081 tgctattgcc ggaatggggg cgttactggc ggcatctatt cgcgcgccgt taacggggat
    19141 cattctggtt ctggagatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    19201 tcttggcgca acactattag cgcaatttac cggcgggaaa ccgctatact cggcgattct
    19261 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    19321 cagcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    19381 gaatttgccc aattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    19441 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    19501 cccgaatctg aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg
    19561 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    19621 cctggtggtt gatccgatga gcctgcaata tctggtcggc ggttccgttg actataccga
    19681 aggactggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    19741 cggttcttcc tttagtatct aatctgttgt tcatgtgccg gataaactat ccggcacatc
    19801 ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    19861 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    19921 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    19981 gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg ccagaacatc acgaatgatc
    20041 ccgccgccaa cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca
    20101 ttaaaggctt tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    20161 ggcaacatcc attttggtaa gtgtcttggc tggcgcacca gcacgatggt cagcatgctg
    20221 gttaccattg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    20281 agcgccatgt cgcgaattgt cccgccgcct acagcggtaa ccacgcccag taccagaaca
    20341 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cggagatggc aaataccgct
    20401 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctaaaaa
    20461 ggtcaggcgc taatctacct gtgaaagcgc attacagagc tgttgtgcag cgaggataat
    20521 acgtgggctt gcacgttcaa accagtcact cgtgagagga ataacgggaa ttttgagctg
    20581 ttcaccccag tattgtttga ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc
    20641 ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc ggccagggaa cccggctgtc
    20701 tttaaagatg ttttctccgc cacaaacttc gagtacctgg ttttgaatcg actcttttcc
    20761 actggtaaat ggcggattaa tgccgaattg cagaaaaaca cgttttttag gtttatcagc
    20821 atattgcgct ttcaattgcg cgtattgatc cagcagggat tgcgcggctt gttcggcctt
    20881 atccggttgc ggactccagg gagccagttg acgtaacgca ttggcaattt gttcaatgtt
    20941 tgtcgcatcg acccacatca cttttattcc cagcgaagcc agctggtcaa cctgccgctc
    21001 ggcattacct ccacgccagg caatcaccag atcgggtttc agcgcgacaa tgcgttccag
    21061 attcatcccc tgccaggtgg aaacctgctc aatcttttgc gcttgtggag gatagtcgga
    21121 atagctgctg accccaaccg gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg
    21181 agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt ggcgcaagaa aagacagggc
    21241 gaccagcgcc ctgaacagtg acttagccat gtgccagttt ctgcactagt gactcaacca
    21301 tcaggctgga ctgtttagcg gcaacagcca ggaactcatc gaagctaaga tgagactgtt
    21361 gatcggccac gtcggagatg gcgcgtacga caacaaacgg gacgttgaaa ttgtggcaga
    21421 catgggcgat tgccgtcgct tccatctcta cagcaatggc ctgtgggaag ttgtggcgga
    21481 ttttcgccag accaacagaa ccgttgatga aagcgtcgcc gctaacaatc aggccacgta
    21541 cagcgttaag attcagttcg gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag
    21601 ctttaaagcc tgccggacag cctggtaact gaccgtattc ataaccaaat gccgtgacat
    21661 ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc gcccactttc agcgttggtg
    21721 ccaggccacc ggcagaaccg gtgttaataa tcacatctgg cttgcagtgt tccaacagca
    21781 gagtggcacc cagcgccgca gcgactttac cgatgcccga tttcagaagc gcaacctcgg
    21841 ttccattcag ttggccggta tagatttcgc aaccgcccag actgatagtt tgacggtttt
    21901 cgattttgtc acgcagcagc gtaacttctt cttccattgc accaatgatg ccgattttca
    21961 tagatttact cgcgataagc ccgatttgaa ggcatagttt accatgcgct tacggggaag
    22021 cgtatttctc acgcgggaga ggacatggca cagattgatt tccgaaaaaa aataaactgg
    22081 catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac atgagatcct gcggatcttc
    22141 gagagcgatc gcgggcgtat catcaactct ccggcaattc gtcgtctgca acaaaagacc
    22201 caggtttttc cactggagcg caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa
    22261 gtccagcagg tggggcgcta catcgccaaa gaaattttaa gccgtctgaa agagcttaaa
    22321 ttgctggaag catacggcct ggatgaactg accggacctt ttgaaagcat tgttgagatg
    22381 tcatgcctga tgcacgatat cggcaatccg ccgtttggtc attttggcga agcggcgata
    22441 aatgactggt ttcgccagcg tttgcacccg gaagatgccg aaagccagcc tctgactgac
    22501 gatcgctgca gcgtggcggt actacgttta cgggacgggg aagaaccgct taacgagctg
    22561 cggcgcaaga ttcgtcagga cttatgtcat tttgagggga atgcacaagg cattcgtctg
    22621 gtgcatacat tgatgcggat gaatctcacc tgggcgcagg ttggcggtat tttaaaatat
    22681 acccgtccag cgtggtggcg tggcgaaacg cctgagacac atcactattt aatgaaaaag
    22741 ccgggttatt atctttctga agaagcctat attgcccggt tgcgtaaaga acttaatttg
    22801 gcgctttaca gtcgttttcc attaacgtgg attatggaag ctgccgacga catctcctat
    22861 tgtgtggcag atcttgaaga tgcggtagag aaaagaatat ttaccgttga gcagctttat
    22921 catcatttgc acgaggcgtg gggccagcat gagaaaggtt cgctcttttc gctggtggtt
    22981 gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc gcagtacgga agatcagttt
    23041 tttatgtatt tacgggtaaa caccctaaat aaactggtac cctacgcggc acaacgattt
    23101 attgataatc tgcctgcgat tttcgccgga acgtttaatc atgcattatt ggaagatgcc
    23161 agcgaatgca gcgatcttct taagctatat aaaaatgtcg ctgtaaaaca tgtgtttagc
    23221 catccagatg tcgagcagct tgaattgcag ggctatcggg tcattagcgg attattagag
    23281 atttatcgcc ctttattaag cctgtcatta tcagacttta ctgaactggt agaaaaagaa
    23341 cgggtgaaac gttcccctat tgaatctcgc ttattccaca aactctcgac gcgccatcgg
    23401 ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt ctcctgagtt tccgctatgg
    23461 gaatattatt accgttgccg cctgctgcag gattatatca gcggtatgac cgacctctat
    23521 gcctgggatg aataccgacg tctgatggcc gtagaacaat aaccaggctt ttgtaaagac
    23581 gaacaataaa tttttacctt ttgcagaaac tttagttcgg aacttcgcgc tataaaacga
    23641 atctgaagaa cacagcaatt ttgcgttatc tgttaatcga gactgaaata catgaaaaaa
    23701 accacattag cactgagtgc actggctctg agtttaggtt tggcgttatc tccgctctct
    23761 gcaacggcgg ctgagacttc ttcagcaacg acagcccagc agatgccaag ccttgcaccg
    23821 atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg tagaaggtag cacaaccgtt
    23881 aatacgccgc gtatgccgcg taatttccag cagttcttcg gtgatgattc tccgttctgc
    23941 caggaaggtt ctccgttcca gagctctccg ttctgccagg gtggccaggg cggtaatggt
    24001 ggcggccagc aacagaaatt catggcgctg ggttccggcg tcatcattga tgccgataaa
    24061 ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga cggtcattaa agttcaactg
    24121 agcgatggcc gtaagttcga cgcgaagatg gttggcaaag atccgcgctc tgatatcgcg
    24181 ctgatccaaa tccagaatcc gaaaaacctg accgcaatta agatggcgga ttctgatgca
    24241 ctgcgcgtgg gtgattacac cgtagcgatt ggtaacccgt ttggtctggg cgagacggta
    24301 acttccggga ttgtctctgc gctggggcgt agcggcctga atgccgaaaa ctacgaaaac
    24361 ttcatccaga ccgatgcagc gatcaaccgt ggtaactccg gtggtgcgct ggttaacctg
    24421 aacggcgaac tgatcggtat caacaccgcg atcctagcac cggacggcgg taacatcggt
    24481 atcggttttg ctatcccgag taacatggtg aaaaacctga cctcgcagat ggtggaatac
    24541 ggccaggtga aacgcggtga gctgggtatt atggggactg agctgaactc cgaactggcg
    24601 aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa gccaggttct gcctaattcc
    24661 tccgctgcaa aagcgggcat taaagcgggt gatgtgatca cctcactgaa cggtaagctg
    24721 atcagcagct ttgccgcact gcgtgctcaa gtgggtacta tgccggtggg cagcaaactg
    24781 accctgggct tgctgcgcga cggtaagcag gtcaacgtga atctggaact gcagcagagc
    24841 agccagaatc aggttgattc cagctccatc ttcaacggca ttgaaggcgc tgagatgagc
    24901 aacaaaggca aagatcaggg cgtggtagtg aacaacgtga aaacgggcac tccggctgcg
    24961 cagatcggcc tgaagaaagg tgatgtgatt attggcgcga accagcaggc tgtgaaaaac
    25021 atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg tgctggcact gaacattcag
    25081 cgcggcgaca gcaccatcta cctgttaatg cagtaatctc cctcaacccc ttcctgaaaa
    25141 cgggaagggg gtctccttac aatctgtgaa cttcaccaca actccataca tcttcatcat
    25201 cctttaggca tttgcacaat gccgtacgtt acgtacttcc ttatgctaag ccgtgcataa
    25261 cggaggactt atggctggct ggcatcttga taccaaaatg gcgcaggata tcgtggcacg
    25321 taccatgcgc atcatcgata ccaatatcaa cgtaatggat gcccgtgggc gaattatcgg
    25381 cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt gcattgctgg tactttcaca
    25441 gggacgagtc gtcgatatcg atgacgcggt ggcacgtcat ctgcacggtg tgcggcaagg
    25501 gattaatcta ccgttacggc tggaaggtga aattgtcggc gtaattggcc tgacaggtga
    25561 accagagaat ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa tgatgctgga
    25621 acagtcgcgg ttgatgcact tgttggcgca ggatagccgt ttgcgggagg aactggtgat
    25681 gaacctgatt caggcagagg agaatactcc cgcacttact gaatgggcgc aacggctggg
    25741 gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg
    25801 tgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta cgcccgagcg
    25861 taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac cggcgttgaa
    25921 ctcttttggg cgctgggatg tagaagatca tcgtaagcga gttgaacaac tgattacccg
    25981 catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt ttaccggtcc
    26041 tggcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg
    26101 gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag
    26161 tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaagcgat
    26221 ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgtcaca atgtgcaacc
    26281 gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc ggcttaatcg
    26341 tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt
    26401 ggcgttacaa ctggatgaag agcggtaggt tatgcgttaa ggttgaggcg gcgctggctc
    26461 attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat tcgggattat
    26521 ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt tacgactgac
    26581 tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct gcggtcacgc
    26641 tggcagattt gatcaagctc atcaatgata taccgtagat tcgggctgat ttcctggact
    26701 tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg cggatattta
    26761 aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat tttcagaata
    26821 tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt ggtaataccc
    26881 agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa atgtgcttat
    26941 tttagcattt ggcctggccc gcccggctcg actgtgatcg cagaaagcct ttttcttcca
    27001 gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg atattccatg
    27061 ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga aacaagatga
    27121 aaaagtattt aaaaaagggg ggaggaagaa tgagtaggac gaattattct gaaccagaaa
    27181 gcaatcgggc agcgtcgtgc tgcccgtgtg catactttta gtcgatggta cgcagcagtt
    27241 cgttaatgcc gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt
    27301 agaggctgta tttgccatct ttcgacggca gattacctga aacaaccaca gaccccgccg
    27361 gaacgcgacc gtagtggatg tcgccggttt cacggtcgta aatacgggtg ctctgaccaa
    27421 tgtatacgcc catggaaatg acggaacctt cttcgacaat caccccttca actacttcag
    27481 agcgcgcgcc gatgaagcaa ttatcttcaa tgatggttgg gttagcctgc agcggttcca
    27541 gcacaccgcc gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag
    27601 aaccgacggt cgcccaggta tcgaccatgg tgccttcatc aacatatgcg ccgatgttga
    27661 cgtaagacgg catcagcacg gtgttacggg caataaacgc accctgacgt accgccgcag
    27721 gtggtacaac gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg gcgaatttca
    27781 tcggcacttt gtcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac
    27841 ggaaagagag cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt
    27901 tttccgctac acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc
    27961 gggtaacggt gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa
    28021 taatgttctg taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt
    28081 tatcgtttgg attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat
    28141 taagtgcacg ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg
    28201 ttgtaattcg ggcaccatga agcgaaattc ccagatcggc aaaaattttc ccgactctcg
    28261 ccagcagtcc tggctggtcg agcgcgataa gctcaaggaa cgatttgcgg tcggtatggg
    28321 ttggcaaaaa cgtcacttcg gtttcaacag taaagtgacg tagtttagcc ggttgacgac
    28381 gcggctgtgg tggctgccag ctactttgtg tcagtacttg ctccagacca aaccgaataa
    28441 cctcatgacg atctgcggat agcgggctac catcgggttc cagcacgata aaggtatcca
    28501 tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg aacgcttaag ttgcgtctat
    28561 ctaactcggc acaaacagca gcaaacaagt aagggcggtc cgggctccag ataaaaatct
    28621 cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct
    28681 gtaataaatg gcgcgcatgc caggccagtt gatttgggct atgacggaca aaatagttcg
    28741 cgcgacagcg tgaccaaatt tggtgcagca cctcttcgtc gatgttatcc atgcgcagta
    28801 gtgccagcgc ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca
    28861 ttccgcgtcg tagctgcttt tcggtggcga aatagagttc gcgcaacaga ctttgcttcc
    28921 agctattcca cagcgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat
    28981 agcgcagacg attttccgtt tgaacttctt cggcaaactg cttgatgact tccgggtcct
    29041 gaatatcgcg acgctgagct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcga
    29101 ccagctgtgt ttcgcgtgag ttcagcccgt ggagttcggc aaaatgcact acatcctgag
    29161 caccgagaat agagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga
    29221 tgaaaatcag ctcagttgac ggtaggcgcg gccagacgtt cacacacaac ggatggcgct
    29281 ggcgcgtttc ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat
    29341 ccaccgtgta ggcgtggaac agatcaaact gcatctgccc gacgatatgt gaccattgcg
    29401 gcatatacgc gccgagtacg ctatggcgat gcattggcaa tagcccgcgc cgcaccgctc
    29461 cggggtgacg tagaatgctc aaaaacagtt ttcgtgcttg cggaatatta cacagcggtt
    29521 gttgcagatg gcgacgggca tggcgtaact ggcgcagtgt ggtggagtaa atgccggtga
    29581 tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa
    29641 atagtgtttc atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgattg
    29701 gacgtggttt ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca
    29761 tctggttgag ttcactgacg cggcgtgtca cgcggaagta atccttcatc atccgctcga
    29821 ccggttcgtt accttcgcca ctgtaattca gacgctgggc gacgctaagc tggcgatcga
    29881 ataacaggcg attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca
    29941 atatatgcag acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccaa
    30001 ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgga
    30061 tatcgcgcaa gccgccaggg ctacttttga tgtctggttc aagattgtag ctggtgccgt
    30121 ggtaacgctg atggcgctgg ttctgttctt caactttcgc cgcgtagaac ttgtcggaag
    30181 gccagaatcc ttcgctgaaa atatgttttt gcagttcaag aaatagcgca acatcgccaa
    30241 ttaataagcg ggattcgatc aaattggtgg cgacggttaa atccgataac ccttccagca
    30301 tgcactcttc aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg
    30361 ttaacagctc gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca
    30421 gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcgacca
    30481 atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt
    30541 cgataaactc ggtgcgcgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat
    30601 cacccagcca acgctggaaa gtatcgatat gggcttttat cccaccgacg gttaattcat
    30661 cacggggcca gacgcatgga ttttgcggtt gaccgggcag ggtggggaga gcggtgtttg
    30721 cgtactgttc tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa
    30781 aaaagccggc attcgccggc ttcattttat tcgtcgtgcg agattatcgc cgggatggtg
    30841 tcatccttgc gtagcgtcag aatttcgcag ccgttatcag tcaccacaat agtatgctca
    30901 tattgtgcag acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg
    30961 atctcttttt tacccgcgtt gaccattggc tcgatggtga acgtcatccc aggtttcagt
    31021 acgacgttgg tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga
    31081 ccaataccgt gtccgcaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc
    31141 tgaatcgccg caccgatttc gcgcagatta atgcctggtt ttaccatgcg tagcgccagg
    31201 tacaggcttt cttgcgtgat acggcacaga cgttcgccca tgatggtcgg cttaccgacg
    31261 ataaacattt tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta
    31321 acgatatcgc catctttcag cagcttagca tcgtccggaa taccgtggca caccacttca
    31381 ttaatagaaa tgcaaacgga tttcggataa ccgtgatagc cgaggcaggc tgaaaccgcg
    31441 tgttgttcat taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc
    31501 ggtttaacat atggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc
    31561 attttttcga tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt
    31621 cggtgatacc gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga
    31681 gaatcattct gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac
    31741 gtctgatgct gtacacagcg ccaacaatta ttggtgtcca cggcgtattt gtggtataaa
    31801 gcgcgccgga cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact
    31861 ttgtgtaaca acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata
    31921 tgggatacgt ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt
    31981 tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac
    32041 ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag
    32101 aaaactgtac cgatgttcaa cgaagctctg gctgaactga acaagattgc ttctcgcaaa
    32161 ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct
    32221 ctgagctgcg accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg
    32281 aaaaccgttc gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt
    32341 actttcgaca agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg
    32401 gaaaacagcc tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc
    32461 gatgctgacc acgaacacat tgctatcaaa gaagcaaaca acctgggtat tccggtattt
    32521 gctatcgttg ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac
    32581 gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc
    32641 cgttctcagg atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc
    32701 ttgataactc ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg
    32761 gagcttacat cagtaagtga ccgggatgag cgagcgaaga tgacgcatct gcggcgcgaa
    32821 atatgaaggg ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat
    32881 atggccccct ttttcacttt tatatctgtg cggtttaatg ccgggcagat cacatctccg
    32941 aggattttag aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg
    33001 gcgcaggcat gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg
    33061 caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg
    33121 ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta
    33181 actgccagac tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc
    33241 tggacgcagc tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag
    33301 aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg
    33361 cgctggaagg cgacgttctg ggttcttatc agcatggtgc gcgtatcggc gttctggttg
    33421 ctgctaaagg cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca
    33481 agccagaatt catcaaaccg gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg
    33541 tacagctgga tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg
    33601 aaggccgcat gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg
    33661 aaccaagcaa aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca
    33721 tccgcttcga agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg
    33781 ctgcgatgtc caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt
    33841 gtgcccatct tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat
    33901 gtcgctagta ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa
    33961 tgcaaaaccc gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac
    34021 tgaaggcttc ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact
    34081 ggttgaactg ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc
    34141 tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac
    34201 cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct
    34261 gatgtccgct attccattga atggcgtgtg cgacagctac agctgggcag aagctatcag
    34321 cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac
    34381 caccgactca gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc
    34441 aaccaaagtt gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg caaccatgta
    34501 cgagcaactg acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc
    34561 cttcacgctg gctcgtgacc ataaattacc gattcgtgtt ttcaacatga acaaaccggg
    34621 tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc
    34681 gtgatggata aataagggta agattccgcg taagtatcgc gggggatctt acccgtaata
    34741 tgtttaatca gggctatact tagcacactt ccactgtgtg tgactgtctg gtctgactga
    34801 gacaagtttt caaggattcg taacgtgatt agcgatatca gaaaagatgc tgaagtacgc
    34861 atggacaaat gcgtagaagc gttcaaaacc caaatcagca aaatacgcac gggtcgtgct
    34921 tctcccagcc tgctggatgg cattgtcgtg gaatattacg gcacgccgac gccgctgcgt
    34981 cagctggcaa gcgtaacggt agaagattcc cgtactctga aaatcaacgt gtttgatcgt
    35041 tcaatgtctc cggccgttga aaaagcgatt atggcgtccg atcttggcct gaacccgaac
    35101 tctgcgggta gcgacatccg tgttccgctg ccgccgctga cggaagaacg tcgtaaagat
    35161 ctgaccaaaa tcgttcgtgg tgaagcagaa caagcgcgtg ttgcagtacg taacgtgcgt
    35221 cgtgacgcga acgacaaagt gaaagcactg ttgaaagata aagagatcag cgaagacgac
    35281 gatcgccgtt ctcaggacga tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa
    35341 gcggcgctgg cagacaaaga agcagaactg atgcagttct gatttcttga acgacaaaaa
    35401 cgccgctcag tagatccttg cggatcggct ggcggcgttt tgctttttat tctgtctcaa
    35461 ctctggatgt ttcatgaagc aactcaccat tctgggctcg accggctcga ttggttgcag
    35521 cacgctggac gtggtgcgcc ataatcccga acacttccgc gtagttgcgc tggtggcagg
    35581 caaaaatgtc actcgcatgg tagaacagtg cctggaattc tctccccgct atgccgtaat
    35641 ggacgatgaa gcgagtgcga aacttcttaa aacgatgcta cagcaacagg gtagccgcac
    35701 cgaagtctta agtgggcaac aagccgcttg cgatatggca gcgcttgagg atgttgatca
    35761 ggtgatggca gccattgttg gcgctgctgg gctgttacct acgcttgctg cgatccgcgc
    35821 gggtaaaacc attttgctgg ccaataaaga atcactggtt acctgcggac gtctgtttat
    35881 ggacgccgta aagcagagca aagcgcaatt gttaccggtc gatagcgaac ataacgccat
    35941 ttttcagagt ttaccgcaac ctatccagca taatctggga tacgctgacc ttgagcaaaa
    36001 tggcgtggtg tccattttac ttaccgggtc tggtggccct ttccgtgaga cgccattgcg
    36061 cgatttggca acaatgacgc ccgatcaagc ctgccgtcat ccgaactggt cgatgggacg
    36121 taaaatttct gtcgattcgg ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg
    36181 ttggctgttt aacgccagcg ccagccagat ggaagtgctg attcacccgc agtcagtgat
    36241 tcactcaatg gtgcgctatc aggacggcag tgttctggcg cagctggggg aaccggatat
    36301 gcgtacgcca attgcccaca ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc
    36361 gctcgatttt tgcaaactaa gtgcgttgac atttgccgca ccggattatg atcgttatcc
    36421 atgcctgaaa ctggcgatgg aggcgttcga acaaggccag gcagcgacga cagcattgaa
    36481 tgccgcaaac gaaatcaccg ttgctgcttt tcttgcgcaa caaatccgct ttacggatat
    36541 cgctgcgttg aatttatccg tactggaaaa aatggatatg cgcgaaccac aatgtgtgga
    36601 cgatgtgtta tctgttgatg cgagcgcgcg tgaagtcgcc agaaaagagg tgatgcgtct
    36661 cgcaagctga ggataatccg gctacagaga gtcgcgctat ttgttagcgt agggcttcag
    36721 tgatatagtc tgcgccatct gatcgtaagt agttggcttt ataaggtcag gtatgccgtg
    36781 gttttacacg gctttttttt gtataggctt cagtattcct gagtaccgta aaccctgtca
    36841 gggaataaaa aacgcgtgat gttgtctgct actcaaccac ttagcgaaaa attgccagcg
    36901 catggctgcc gtcatgttgc gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa
    36961 gggaagattc gtgcctttgg gcataaagcc ggagcaaaat ccgtccgccg ggctgtctct
    37021 tttgcggcca acaacggtat tgaggcgtta acgctgtatg cctttagtag tgaaaactgg
    37081 aaccgaccag cgcaggaagt cagtgcgtta atggaactgt ttgtgtgggc gctcgatagc
    37141 gaagtaaaaa gtctgcaccg acataacgtg cgtctgcgta ttattggcga taccagtcgc
    37201 tttaactcgc gtttgcaaga acgtattcgt aaatctgaag cgctaacagc cgggaatacc
    37261 ggtctgacgc tgaatattgc ggcgaactac ggtggacgtt gggatatagt ccagggagtc
    37321 aggcaactgg ctgaaaaggt gcagcaagga aacctgcaac cagatcagat agatgaagag
    37381 atgctaaacc agcatgtctg tatgcatgaa ctggcccctg tagatttagt aattaggact
    37441 gggggggagc atcgcattag taactttttg ctttggcaaa ttgcctatgc cgaactttac
    37501 tttacagatg ttctctggcc cgatttcgat gaacaagact ttgaaggggc gttaaatgcc
    37561 tttgctaatc gagagcgtcg tttcggcggc accgagcccg gtgatgaaac agcctgatgg
    37621 gggtcgcttt tgctgaagta tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc
    37681 gcggcgttgt ttctgttgcc gccggtgggg ttcgccattg taacgctggt ggtctgcatg
    37741 ctggcagcgt gggaatgggg acagcttagc ggttttacca ctcgttcgca gcgagtatgg
    37801 ttggcggtgt tatgcgggtt attgttggcg ctgatgcttt ttctgttgcc ggaatatcac
    37861 cgaaatattc atcaaccgct ggttgaaatc tcactttggg cttcgctggg ttggtggatt
    37921 gtcgcgctat tgctggtgct gttttaccca ggttccgcag caatctggcg taactctaaa
    37981 acattgcgcc ttatttttgg cgtgctaacc attgttccct tcttctgggg catgctggcg
    38041 ttacgggcct ggcactatga cgagaatcat tacagtggcg caatatggct gctctatgtc
    38101 atgatcctgg tatggggcgc tgactccggc gcatatatgt ttggcaaatt gtttggtaaa
    38161 cataagctgg caccgaaggt ttctccgggt aaaacctggc aaggctttat cggtggactc
    38221 gctactgcag cggtaatctc atggggttat ggcatgtggg cgaatctcga cgtcgctccc
    38281 gtcaccttac tcatttgctc tattgtcgca gcgttagcct cagtgctcgg cgatctgacc
    38341 gagagtatgt ttaagcgtga agcaggaatt aaggacagcg gtcatttaat tccaggacac
    38401 ggtggtattt tagatcgtat tgatagcctg acggctgcgg taccggtctt tgcttgcttg
    38461 ttgttactgg tattcaggac gctttaacgg aaggtaatat gctgagtttt ctctgggatt
    38521 tggcttcgtt catcgttgca ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct
    38581 gggttgcccg gcgttgtggt gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc
    38641 tctggcggcg aactgataag ctcggcaccg aatatgttat cgccctgatc ccgttgggcg
    38701 gttatgtcaa aatgctggat gagcgcgcag aaccggtcgt tccggaactc cgccaccatg
    38761 ccttcaataa taaatctgtc ggccaacgag cggcgattat tgccgcaggt ccggttgcaa
    38821 acttcatttt tgctatcttt gcctactggc tggtttttat tattggtgtg cctggcgtac
    38881 gtccggtggt tggcgaaata gcagccaatt cgatagctgc ggaagcacaa attgcaccag
    38941 gtacggaact aaaagccgta gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc
    39001 agttggtcga taaaattggc gatgaaagca ccaccattac agtagcgcca tttggcagcg
    39061 accaacggcg ggatgtaaag ctcgatttac gtcactgggc gtttgagcct gataaagaag
    39121 atccggtatc ttcgctgggg attcgtcctc gtgggccgca aattgaacct gtactggaaa
    39181 atgtgcagcc aaactcggcg gcaagcaagg caggtttgca agcaggcgac aggatcgtta
    39241 aagtcgatgg tcagccctta acgcagtggg tgacctttgt gatgcttgtc cgggataacc
    39301 cgggtaaatc cttagcgtta gaaatcgaaa ggcaggggag tcccttgtct ttgacattaa
    39361 tcccggagag taaaccgggt aatggtaaag cgattggttt tgtcggtatt gagccgaaag
    39421 tcattccttt gccagatgag tataaagttg tacgccagta tgggccgttc aacgccatcg
    39481 tcgaagccac ggacaaaacg tggcagctga tgaagctgac ggtcagtatg ctgggaaaat
    39541 tgatcaccgg tgatgtgaaa ctgaacaacc tcagtgggcc gatctctatc gccaaggggg
    39601 ctgggatgac agcggaactc ggggtagttt attacctgcc gtttcttgcg cttattagcg
    39661 tgaacttagg gataattaac ctgtttccgt tgcccgtact tgacgggggg catctgctgt
    39721 tccttgcgat cgaaaagatc aaaggcggac cggtatccga gcgggttcaa gacttttgtt
    39781 atcgcattgg ctcgattctg ctggtgctgt taatggggct tgcacttttc aatgatttct
    39841 ctcggttatg agagttagtt aggaagaacg cataataacg atggcgatga aaaagttgct
    39901 catagcgtcg ctgctgttta gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa
    39961 agatattcat ttcgaaggcc ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc
    40021 ggtgcgcaca ggcgacacgg ttaatgatga agatatcagt aataccattc gcgctctgtt
    40081 tgctaccggc aactttgagg atgttcgcgt ccttcgtgat ggtgataccc ttctggttca
    40141 ggtaaaagaa cgtccgacca ttgccagcat tactttctcc ggtaacaaat cggtgaaaga
    40201 tgacatgctg aagcaaaacc tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg
    40261 caccaccatt gccgatatcg agaaaggtct ggaagacttc tactacagcg tcggtaaata
    40321 tagcgccagc gttaaagctg tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact
    40381 ggtgttccag gaaggtgtgt cagctgaaat ccagcaaatt aacattgttg gtaaccatgc
    40441 tttcaccacc gacgaactga tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa
    40501 cgtggtaggc gatcgtaaat accagaaaca gaaactggcg ggcgaccttg aaaccctgcg
    40561 cagctactat ctggaccgcg gttatgcccg tttcaacata gactctaccc aggtcagtct
    40621 gacgccagat aaaaaaggta tttacgtcac ggtgaacatc accgaaggcg atcagtacaa
    40681 gctttctggc gttgaagtga gcggcaacct tgccgggcac tccgctgaaa ttgagcagct
    40741 gactaagatc gagccgggcg agctgtataa cggcaccaaa gtgaccaaaa tggaagatga
    40801 cattaaaaag cttctcggtc gctatggtta tgcctatccg cgcgtacagt cgatgcctga
    40861 aattaacgat gccgacaaaa ccgttaaatt acgcgtgaac gttgatgcgg gtaaccgttt
    40921 ctacgtgcgt aagatccgtt ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg
    40981 cgaaatgcgt cagatggaag gtgcatggct gggaagcgat ctggtcgatc agggtaagga
    41041 gcgtctgaat cgtctgggct tctttgaaac tgtcgatacc gatacccaac gtgttccggg
    41101 tagcccggac caggtagatg tagtctacaa ggtgaaagaa cgtaacactg gtagcttcaa
    41161 cttcgggatt ggttacggta ctgaaagtgg cgtgagcttc caggctggcg tgcaacagga
    41221 taactggtta ggtacaggtt atgctgttgg tatcaacggg accaaaaacg attaccagac
    41281 ctatgctgaa ctgtcggtaa ccaacccgta cttcaccgta gatggcgtaa gcctcggtgg
    41341 tcgtctcttc tataatgact tccaggcaga tgacgccgac ctgtccgact ataccaacaa
    41401 gagttatggt acagacgtga cgttgggctt cccgattaac gaatataact cgctgcgtgc
    41461 aggtctgggt tatgtacata actccctgtc caacatgcag cctcaggttg cgatgtggcg
    41521 ttatctgtac tctatgggtg aacatccgag cacctctgat caggataaca gcttcaaaac
    41581 ggacgacttc acgttcaact atggttggac ctataacaag cttgaccgtg gttacttccc
    41641 gacagatggt tcacgtgtca acctgaccgg taaagtgacc attcctggat cggataacga
    41701 atactacaaa gtgacgttag acacggcgac ttatgtgccg atcgatgacg atcacaaatg
    41761 ggttgttctg gggcgtaccc gctggggtta tggtgatggt ttaggcggca aagagatgcc
    41821 gttctacgag aacttctatg ccggtggttc cagcaccgtg cgtggcttcc agtccaatac
    41881 aattggtccg aaagcagttt acttcccgca tcaggccagt aattatgatc cggactatga
    41941 ttacgaatgt gcgactcagg acggcgcgaa agacctgtgt aaatcggatg atgctgtagg
    42001 cggtaacgcc atggcggttg ccagcctcga gttcatcacc ccgacgccgt ttattagcga
    42061 taagtatgct aactcggttc gtacttcctt cttctgggat atgggtaccg tttgggatac
    42121 aaactgggat tccagccaat attctggata tccggactat agtgatccaa gcaatatccg
    42181 tatgtctgcg ggtatcgcat tacaatggat gtccccattg gggccgttgg tgttctccta
    42241 cgcccagccg ttcaaaaagt acgatggaga caaggcagaa cagttccagt ttaacatcgg
    42301 taaaacctgg taagtgttct ccacaaagga atgtagtggt agtgtagcga tgactttagg
    42361 cgatcaatat aaatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg
    42421 taaggagttt attgtgaaaa agtggttatt agctgcaggt ctcggtttag cactggcaac
    42481 atctgctcag gcggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt
    42541 agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga
    42601 actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc
    42661 gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca
    42721 gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aatgaagaac gcggcaaact
    42781 ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt
    42841 tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt
    42901 actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt
    42961 ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc
    43021 tgcacaaaca ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt
    43081 gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc
    43141 gctggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac
    43201 gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg
    43261 taacaacgta tcgattggcg ctaacgcggt gattgaatcc ggcgttgaac tgggcgataa
    43321 cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg
    43381 tctctgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca
    43441 gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt
    43501 gaagatccca cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac
    43561 taccatcgat cgcggcgcgc tggatgacac tgttattggc aatggcgtga tcattgataa
    43621 ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt
    43681 cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat
    43741 caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg
    43801 tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg
    43861 gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct
    43921 tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc
    43981 ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac
    44041 aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt
    44101 ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt
    44161 tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct
    44221 ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt
    44281 ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgctggtatt
    44341 gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact
    44401 ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgacggtaaa
    44461 gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat
    44521 aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat tggcgcgaac
    44581 gcacacattg gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta
    44641 ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat
    44701 cagttcgcct ccatcggcga agttaaccag gatctgaaat atgctggcga accgacccgc
    44761 gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc
    44821 cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt
    44881 gcgcacgatt gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt
    44941 cacgtatcgg ttgacgactt cgcgatcatc ggcggcatgg ccgcagtcca tcagttctgc
    45001 atcattggtg cgcatgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct
    45061 tatgtcattg cgcagggtaa ccacgccacg ccgttcggtg tcaatatcga agggctgaag
    45121 cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa gctgatttat
    45181 cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat
    45241 ccggaagtga aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa
    45301 tgactgaaca gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc
    45361 tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg
    45421 ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagagttgg
    45481 cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg
    45541 atctgacaaa gcgttttggt gaactaaagc cagatgtttt tgttggcatt gatgcgcctg
    45601 acttcaatat tatcctcgaa ggtaacctta aaaagcaggg tatcaaaacc attcattatg
    45661 tcagtccgtc cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg
    45721 atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aacgtaccgt
    45781 gccgctttat cggtcatacc atggctgatg ccatgccatt agatccagat aaaaatagtg
    45841 cccgtgatgt gctggggatc ccttacgatg cccactgtct ggcattgttg ccgggcagcc
    45901 gtggtgcaga agtcgaaatg cttagtgccg atttcctgaa aacggcccag cttttgcgcc
    45961 agacatatcc ggatctcgaa atcgtggtgc cactggtgaa tgccaaacgc cgcgagcagt
    46021 ttgaacgcat caaagctgca gtcgcgccag acctgtcggt tcatttgctg gatggaatgg
    46081 gccgtgaggc gatggtcgcc agcgatgcgg cactactggc gtcggggacg gcagccctgg
    46141 agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct
    46201 ggttggcgaa gcggctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggca
    46261 gagagttagt caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc
    46321 tgttaccgct gttggcgaat gggaaaacca gccacgcgat gcacgatacc ttccgtgaac
    46381 tgcatcagca gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag
    46441 cacaatgatc gaatttgtct atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg
    46501 acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg attctcgacc cggcgcgccc
    46561 gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgtcgtctgg cgctctgtga
    46621 agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacctc acgaaatcga
    46681 cgaactgaat attttacatg cgaccatgct ggcgatgcag cgagccgtcg ctgggctgca
    46741 tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc
    46801 gatggctgtg gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt ctatcctggc
    46861 gaaagtgacg cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt
    46921 tgcccaacac aaagggtatc caaccgcttt tcatctggaa aaactggctg aacacggcgc
    46981 gaccgaacac catcggcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg
    47041 attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca
    47101 cctgcgggtg cacagcgact actcgatgat cgatggcctg gccaaaaccg caccgctggt
    47161 aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg
    47221 cggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc
    47281 agattttaac gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc
    47341 ggcgaacaat accggctatc agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg
    47401 gtacggtgcc gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt
    47461 gatccttctt tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag
    47521 cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct
    47581 cgagctgatc cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact
    47641 ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag
    47701 cgactttgac gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc
    47761 taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgcga
    47821 gctgtttgcc gacatcccag aagcccttgc caacaccgtt gagatcgcca aacgctgtaa
    47881 cgtaactgtg cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac
    47941 cgaagattat ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt
    48001 ccctgatgag gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc tggagactga
    48061 acttcaggtt atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat
    48121 ccagtggtcg aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc
    48181 actggtggcc tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt
    48241 cgaacgtttc cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat
    48301 ggagaaacgc gatcaggtta tcgaacacgt agcggacatg tacggtcgtg atgcggtatc
    48361 gcagattatc accttcggta cgatggcggc gaaagcggtg atccgcgacg taggccgcgt
    48421 gctggggcat ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg
    48481 gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga
    48541 tgaagaagtt aaggcgttga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc
    48601 cggtaagcac gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct
    48661 ttattgcgat gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata
    48721 cgccggactg gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc
    48781 gctggagatg atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg atatcgccgc
    48841 gatcccgctg gatgacaaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt
    48901 attccagctt gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt
    48961 cgaagatatg atcgcactgg tggcactgtt ccgccctggt ccgttgcaat cagggatggt
    49021 ggataacttt atcgaccgta aacatggtcg cgaagagatc tcctatccgg acgtgcagtg
    49081 gcagcatgaa agcctgaaac cggtactgga gccaacctac ggcattatcc tgtatcagga
    49141 acaggtcatg cagattgcgc aggtgctttc tggttatacc ctcggtggcg cggatatgct
    49201 gcgtcgtgcg atgggtaaga aaaagccgga agagatggct aagcaacgtt ctgtatttgc
    49261 tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt
    49321 ggagaaattc gctggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc
    49381 atatcaaaca ttatggttga aagcgcacta tccggcagag tttatggcgg cggtaatgac
    49441 cgccgatatg gacaacaccg agaaggtggt gggcctggtg gatgagtgct ggcggatggg
    49501 gctgaaaatc ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga
    49561 cggcgaaatc gtgtatggta ttggcgcgat caaaggggta ggtgaaggtc cgattgaggc
    49621 gatcatcgaa gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg
    49681 aaccgacacc aaaaagttga accgtcgcgt gctggaaaaa ctgatcatgt ccggggcgtt
    49741 tgaccgtctt gggccacatc gcgcggcgct gatgaactcg ctgggcgatg cgttaaaagc
    49801 ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc
    49861 cgaagagccg gaacaaattg aacaatccta tgccagctgc caaccgtggc cggagcaggt
    49921 ggtattagat ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca
    49981 gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac
    50041 agaacgtggt aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac
    50101 caagcgcggc aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt
    50161 gatgttgttt actgacgccc tggataaata ccagcatttg ctggaaaaag accgcatact
    50221 tatcgtcagc ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg
    50281 cgaagtgatg gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct
    50341 gacggacagg caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca
    50401 ccgctctggg acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt
    50461 gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg
    50521 cctcattggt tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct
    50581 gaatttcctt gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac
    50641 tgcggttagc cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct
    50701 gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat
    50761 tgcgcaactg gcacgccatc cacagcgtcc ttataccctg gattacgttc gcctggcatt
    50821 tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg
    50881 tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga
    50941 aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc
    51001 actgcgtctg atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac
    51061 cccgggggct tatccgggcg tgggcgcaga agaacgtggt cagtctgaag ccattgcacg
    51121 caacctgcgt gaaatgtctc gcctcggcgt accggtagtt tgtacggtta tcggtgaagg
    51181 tggttctggc ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag
    51241 cacctattcc gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa
    51301 agcgccgctg gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact
    51361 gatcgactcc atcatcccgg aaccgctggg cggcgctcac cgtaacccgg aagcgatggc
    51421 ggcatcgttg aaagcgcaac tgctggcgga tctggccgat ctcgacgtgt taagcactga
    51481 agatttaaaa aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa
    51541 agttctgaaa aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat
    51601 gattaaggaa ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat
    51661 ggcgtctttt ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc
    51721 tttcagatta tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct
    51781 cgaatttgcg gcgtgatttt tgactgggat gaatacagtc tcgatttatg tagcgatatc
    51841 aatcagctta atgaatatct cccgctttat gccttcatca acacccactc gacgatggat
    51901 gtcagcgtgc aggatatgcg gatggcgctc tgtttttttg aatatgcgct ggggcaggcg
    51961 gaagatatcg ccattcgtat gcgtcagtac accgacgaat atcttgataa cattacccca
    52021 ccgttcacga aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg
    52081 ggtcatatgg gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgacttt
    52141 ttcggcggga atacccttaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg
    52201 ctcgaccaca ccgggccaca cctggaagcg gaagagtaca tcgcgcggac tttcggcgcg
    52261 gaacagagtt atatcgttac caacggaaca tcgacgtcga acaaaattgt gggtatgtac
    52321 gccgcgccat ccggcagtac gctgttgatt gaccgcaatt gtcataaatc gctggcgcat
    52381 ctgttgatga tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg
    52441 attctgggtg ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct
    52501 gccaccacgc aggcacaatg gccggttcat gcggtgatta ccaactcaac ctatgatggc
    52561 ttgctctaca acaccgactg gatcaaacag acgctggatg tcccgtcgat ccacttcgat
    52621 tccgcctggg tgccgtacac ccattttcat ccgatctacc aggggaaaag tggtatgagc
    52681 ggcgagcgtg ttgcgggaaa agtgatcttc gaaacgcaat cgacccacaa aatgctggcg
    52741 gcgttatcgc aggcttcgct gatccacatt aaaggtgagt atgacgaaga ggcgtttaac
    52801 gaagccttta tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag
    52861 acggcagcag cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcagtagaa
    52921 cgagctctgc attttcgcaa agaggtccag cggctgcggg aagagtctga cggttggttt
    52981 ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgcctggc
    53041 gaacaatggc acggctttaa cgatgcggat gccgatcata tgttcctcga tccggttaaa
    53101 gtcactattc tgacgccggg gatggacgag cagggcaata tgagcgagga ggggatcccg
    53161 gcggcgctgg tggcgaagtt cctcgatgag cgtgggatcg tggtagagaa aaccggccca
    53221 tataacctgc tgtttctctt tagtattggc atcgataaaa ccaaagcaat gggattattg
    53281 cgtgggttga cggaatttaa acgctcttac gatctcaatc tgcgaatcaa aaatatgctt
    53341 ccggatctgt acgcagaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg
    53401 caagggatcc ataagctgat tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc
    53461 gatactttgc cggagatgat catgacgcca catcaggcat ggcagagaca gattaaaggt
    53521 gaagtagaaa ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg
    53581 ccttatccac cgggcgtacc gctgttgatg cctggagaaa tgctgaccaa agagagccgc
    53641 acagtactcg attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa
    53701 acggatattc acggtgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa
    53761 atggcgggat aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag
    53821 gagacgttat gctgggttta aaacaggttc accatattgc gattattgcg acggattatg
    53881 cggtgagcaa agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc
    53941 gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc
    54001 ttttctcatt tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc
    54061 atctggcttt tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg
    54121 tgaagtgtga agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg
    54181 atccggacgg gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc
    54241 ccggtaacgt gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat
    54301 agacaacttc tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc
    54361 gttctgctgc atcagttggt gcagtggcgg acggaaaatc cggggggcgc tctgcgcgct
    54421 attcatgtgc atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac
    54481 gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg
    54541 ctggggattg aggcccaggc gcggcaggcg cgttatcagg catttgcccg caccttgttg
    54601 cccggtgaag tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ttttctgctg
    54661 gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt
    54721 gccggaacac ggcttatccg cccgttgctt gcccgcacgc ggggggaact ggcgcagtgg
    54781 gcattggcgc atggtttacg ctggattgaa gacgaaagta atcaggacga ctcatacgat
    54841 cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcc
    54901 gaaacaacgg cccgcagcgc cgcactttgt gctgaacaag agagcctgct ggatgaactg
    54961 ctggcagatg atttagcaca ctgtcaatcg ccgcagggga cgctgcagat tgtgccaatg
    55021 ctggcgatga gtgatgcccg ccgcgcggcg attatccgcc gctggctggc agggcagaat
    55081 gcaccgatgc cttcccgcga cgcgttggtg aggatctggc aggaagtggc gctggcgcgg
    55141 gaagatgcct ccccctgttt acgtttgggc gcgtttgaaa tccgacgcta tcagtcgcaa
    55201 ctgtggtgga ttaaatccgt caccgggcaa agcgaaaaca ttgtgccgtg gcagacatgg
    55261 cttcaaccgc tggaattacc ggcggggcag ggaagtgtac agcttaatgc gggaggcgat
    55321 attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg
    55381 catattgtcg ggcgtaacgg cggacgtaag ctaaagaaaa tctggcaaga gctgggcgtg
    55441 ccgccgtggc tacgtgacac cacgccactg ctgttttatg gcgaaacgct gattgcggcg
    55501 gcagggggat ttgtgacgca agaaggtgtg gctgaaggtg agaatggcat aagttttgtc
    55561 tggcagcgaa acgcttagtt tagtgaaggc cggatatgac gcattaagcg tcgcatccgg
    55621 cgaaagtcaa tcaggactcg ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg
    55681 taattttatc cagacgtagc tcacgggttt cgccagcggc ctcgacgacc agatactcca
    55741 catttttgcg ggagactaaa tcgctggctt tggcctgcaa tttttcgcca tctttcagct
    55801 caagtgtcag cattaaatga tgctggcagg cgagctcaag attatcgtaa tcatcacaat
    55861 tgattggttg atacgtatca ttcattgaca taatcgctca ccagtaagtt tgccgcagcg
    55921 tatgctgctt tttccctgac agcctcagaa agggcgtcgt cggcagccat ttcattcagc
    55981 actttcaaaa cgcagcccag cgcgtccgga acgtatccta agtctccgct ggcgatttcc
    56041 gcgtaccgct tgcgtattaa ctcacaatat ttttccacat gccctcctgt cagcactctg
    56101 acttaaccgt ggatgcaagt ctaagcctac gaagataaac tctgtttcgc aaggtgacta
    56161 taccacactc atttctgcaa tatcagcgcc gcaactgcac gtattccgtt acaatggcct
    56221 cctgattcga aaggagtttt cttatggcgc ttaaagcgac aatttataaa gcgacggtta
    56281 atgtggccga tctcgaccgc aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc
    56341 cttcagaaac ccaggagcgt atgatgctgc gcctgctggc gtggctgaaa tatgctgatg
    56401 aacgtctgca atttacccgt ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta
    56461 acgatcacct gggcattgat ttgtggattg agctggggct gccggatgag cgacgaatta
    56521 agaaagcctg cacccaggcc gcagaagtgg cgctgtttac ctataatagt cgggcggcgc
    56581 aaatctggtg gcagcaaaat cagagcaaat gtgcgcagtt tgccaatctt tccgtctggt
    56641 atctggacga cgagcagctg gcgaaagtga gcgcctttgc cgatcgtacc atgacgctgc
    56701 aggcaacgat tcaggatggc gtgatctggt tatcggatga taagaataat ctggaagtga
    56761 acttaaccgc ctggcaacaa ccttcatgat tgtgatttcc cgacatgttg ctattcccga
    56821 tggtgagctt gagatcaccg ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa
    56881 gacctcaacg gccattcatc tgcgttttga cattcgggca tccagcctgc cagagtatta
    56941 caaagagcgt ctgctcgccg ccagccatca tttgatcagc agtgatggcg taattgtcat
    57001 taaggcacag gaataccgca gtcaggaact gaaccgcgaa gcggcgctgg cccggctggt
    57061 ggcagtgatt aaagatttaa caacagaaca aaaagcccga cgacccacgc ggcccacccg
    57121 tgcatcgaaa gagcgcaggc tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg
    57181 cagcggtcgg gaataaaaag aaggaatgga tggtgaaaaa agcgatagtg acagcgatgg
    57241 ctgtaatcag cctctttact ctgatgggtt gtaataatcg ggccgaagtc gatacgcttt
    57301 ctccggcgca ggctgccgaa ttgaaaccga tgccgcaaag ttggcgcggc gtgctgccgt
    57361 gtgccgattg cgaaggaatc gaaacctctc tgttcctcga aaaagacgga acatgggtga
    57421 tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc tacggtacat
    57481 gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa aggtgaaaag tcatattatc
    57541 gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg caatccgatt gaatcgcagt
    57601 tcaactatac gctggaaccg gcacaatcca gtttacccat gacgccgatg accctgcggg
    57661 gcatgtattt ttatatggct gatgcggcga ccttcactga ttgcgcgacc ggaaaacgtt
    57721 tcatggtagc gaataacgca gagctggagc gtggctacct ggctgcgcgc ggccacagtg
    57781 aaaaaccggt gttactgtca gtagaaggtc actttacgct tgaggctaat ccggataccg
    57841 gtgcgccaac caaagtatta gcacccgata cggcagggaa attttaccca aaccaggatt
    57901 gcagtagttt ggggcagtaa cccgtcttga gacagaaaca aacgcaaaac aggccagaag
    57961 gatatatttc aacattttga atttgcacgt tttttgtagg ccggataagg cgtttacgcc
    58021 gcatccggca atggtgctca acgcctgatg cgacgctggc gcgtcttatc atgcctacaa
    58081 cccccctcat accttaaggc ggataaggca tttactttat caccgggtca aaccaacaaa
    58141 gcgacccgcc ttgatataaa tccctggctt caggtgccca acatactgta actgcgtttc
    58201 tggcttaaac gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca
    58261 gcccgattgc gccagcgtaa gcggatgccc ggctttggca aatgcatcag tgacaaattc
    58321 cgaacaaaac cacgactttt tgtctccttc gaccacactg cttaactgcg ctttcgccag
    58381 gccgctgacg cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg
    58441 agtcaccata aagggaataa attcgacaat gccgcgatag ttataaccgc tatctttgat
    58501 tttattggcg aacgcggtga tttctgtggc ttgttgcggg gtaagatccg ggactcgtaa
    58561 gacgaaaagt ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc
    58621 gcctgtcgct tctgcaacgt tattttcacc cagatagatt gcaacgtgac tcacagagga
    58681 agtgctgaaa acgcggatcc caaatgaggt tacctccagg cttgaggaga acagcaaatc
    58741 gccgggtttg agatcaggta ccgttatttc ttttattgat tgttcggtga aagagctttg
    58801 atgctggaat ttgacagccc atgttttcgc ctcggcatct actgctgtgg ctgatgagtc
    58861 tggctggctg atatcaacgg tacaggccga aagtaaaaga aagcagggga gaagcaggcg
    58921 gcagtacgcc tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc
    58981 aggacccttt ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag
    59041 tcttaattaa ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga
    59101 ggttacggtc gcccagcaca atagtgtgcg gaataccgat cagttccata tcggcaaaca
    59161 tcacgcccgg acgttctttt cggtcatcca gcagcacttc gataccttgt gcacgcagtt
    59221 cgctgtacag tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca
    59281 gaattgccac ctggaacggc gcgatagcgt caggccatac gatgccgcgc tcgtcgtagt
    59341 tctgctcaat cgccgcagcc actacgcgcg ttaccccgat accgtagcaa cccatagtca
    59401 ggatttggtt acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg
    59461 tacccagctg gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc
    59521 catccgggct tggatcgcca gccaccacgt tacggatatc cgcaacttcc ggggtagcga
    59581 catcgcgatc ccagttgatg ccgaagtagt gtttaccatc gatgttagca ccagcagtga
    59641 aatcactcat cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac
    59701 ccagtgaacc cggaccggct ttaaccacgg cacgaatttc ttcttcggtc gcgaaagtca
    59761 gcgggctggc aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc
    59821 gcaccagcag cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct
    59881 taaccgtttt cttaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt
    59941 tcggcgtatc aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga
    60001 tagcttctgc cagctcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt
    60061 cttcaccgct ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg
    60121 tgtcggcctg tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg
    60181 catacattgc atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat
    60241 ctttcatcag gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga
    60301 acttggtctg gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac
    60361 gaatcaggtc agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac
    60421 ggtcaacaaa acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca
    60481 aatcggctgg ctgaaccacc ggcatcgaca cctcgattgc accggcgttg ttcatctctt
    60541 cacgcacgat gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata
    60601 acccggaggc cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct
    60661 cggcgtcggc aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt
    60721 tacggttcca gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt
    60781 tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag
    60841 cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca
    60901 gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc
    60961 cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg
    61021 cttttcgacc tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata
    61081 gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac
    61141 cggcgtacca tcgaccagat ccaggctgcc gagcttcaga atcacgcagt ctttatggca
    61201 aacaacctct ttcagctcta ccagcgacat accaatcggg ttggggcgga aagtggagcg
    61261 cgtagcgaaa acacccattc tggcattacc gccaagacga ggcggacgca cagtcggacg
    61321 ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc
    61381 caggccgcga acggcgtcag cctggttgta gggagcaatg agatgtagtt cgccgttggc
    61441 gctttttacc agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat
    61501 aacacctatt tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagaaccg
    61561 atacataaag cctgacgata gcagcctggc gtaccgctgg taacttcgca gctatgcagt
    61621 aataccgcat tggctttcat tttcgaggcg ttgatttgca tccgcttacg cgcggttgga
    61681 atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga
    61741 tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg
    61801 cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt
    61861 tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc
    61921 ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag
    61981 cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa
    62041 cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg
    62101 taaacttcgt cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta
    62161 tcttcacgcg ttacgatcag gtttacgtac ggggagtctt tatcttcgac aaagataccg
    62221 tttttcgctg gagtcaggcc aatctggctg gcataggtgg tgttaataac tgccagagcg
    62281 atttgcgcgt cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga
    62341 ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt
    62401 ttgatcaagc ccactttttg cagcagcagc agtgaacgac caaggttagt tggatcgttt
    62461 ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa
    62521 ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc
    62581 agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc
    62641 aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat
    62701 ttgtctttcg caactttctg cgcgacttct gcaacctgct gttcggcacc aacaatcacg
    62761 ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat
    62821 ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc
    62881 ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg
    62941 cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc
    63001 gtcgcgttat agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg
    63061 actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc
    63121 accagacccg gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca
    63181 cccattgcgc gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc
    63241 atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca
    63301 atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac
    63361 gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca
    63421 ataatttgcc ccggacgcgt gacataaagc aggacgccaa ccggcagacc aaccacaaac
    63481 ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc
    63541 cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca
    63601 ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa
    63661 cttaacgcca ccggcgtaat ccatctgcgc gctaataatg ttgttgttga cgttgaaacg
    63721 acgcgcggtt tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag
    63781 catcggcacg cagtcagtaa atggctccgc ttgcagacgt tcctggtaat cttccgggat
    63841 atccagatgc agggttgact gaataaactt ctgcgccagc ggcgttttcg gatgtgagaa
    63901 cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg caacgcaatc
    63961 acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg
    64021 gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt ctagcgcgct
    64081 ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg caatcgccac
    64141 acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat ctccaagacc
    64201 aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg tattgtccag
    64261 ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg
    64321 gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag
    64381 ttcctgtcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg
    64441 tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg
    64501 cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt
    64561 ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtggca
    64621 tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt
    64681 ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag
    64741 cgtcacatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct
    64801 tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga
    64861 atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt
    64921 ggtagtcacc aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac
    64981 gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta
    65041 ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct gcgattgccg
    65101 caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc
    65161 ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg
    65221 aacaaaagtg ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga
    65281 ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc
    65341 gcaatgatta aaagatgagc ggttgaaata aaaatgcatt tttccgcttg tcttcctgag
    65401 ccgactccct ataatgcgcc tccatcgaca cggcgaatgt gaatcacttc acacaaacag
    65461 ccggttcggt tgaagagaaa aaatcctgaa attcag
//
LOCUS       DBFIFS010000005        65385 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.005, whole genome shotgun sequence.
ACCESSION   DBFIFS010000005 DBFIFS010000000
VERSION     DBFIFS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.005"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            235..1932
                     /gene="ypdA"
                     /locus_tag="N7A07_000282"
     CDS             235..1932
                     /gene="ypdA"
                     /locus_tag="N7A07_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU2475032.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1947..2681
                     /gene="ypdB"
                     /locus_tag="N7A07_000283"
     CDS             1947..2681
                     /gene="ypdB"
                     /locus_tag="N7A07_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU2475033.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2694..3551
                     /gene="ypdC"
                     /locus_tag="N7A07_000284"
     CDS             2694..3551
                     /gene="ypdC"
                     /locus_tag="N7A07_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2475034.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3554..6049)
                     /gene="ptsP"
                     /locus_tag="N7A07_000285"
     CDS             complement(3554..6049)
                     /gene="ptsP"
                     /locus_tag="N7A07_000285"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2475035.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(6074..7111)
                     /gene="ypdE"
                     /locus_tag="N7A07_000286"
     CDS             complement(6074..7111)
                     /gene="ypdE"
                     /locus_tag="N7A07_000286"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2475036.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7111..8196)
                     /gene="ypdF"
                     /locus_tag="N7A07_000287"
     CDS             complement(7111..8196)
                     /gene="ypdF"
                     /locus_tag="N7A07_000287"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2475037.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8211..9458)
                     /gene="fryC"
                     /locus_tag="N7A07_000288"
     CDS             complement(8211..9458)
                     /gene="fryC"
                     /locus_tag="N7A07_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU2475038.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9480..9806)
                     /gene="fryB"
                     /locus_tag="N7A07_000289"
     CDS             complement(9480..9806)
                     /gene="fryB"
                     /locus_tag="N7A07_000289"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU2475039.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(10025..10990)
                     /gene="glk"
                     /locus_tag="N7A07_000290"
     CDS             complement(10025..10990)
                     /gene="glk"
                     /locus_tag="N7A07_000290"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU2475040.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11194..12450
                     /gene="yfeO"
                     /locus_tag="N7A07_000291"
     CDS             11194..12450
                     /gene="yfeO"
                     /locus_tag="N7A07_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU2475041.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12565..12891
                     /gene="ypeC"
                     /locus_tag="N7A07_000292"
     CDS             12565..12891
                     /gene="ypeC"
                     /locus_tag="N7A07_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU2475042.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(13031..14269)
                     /gene="mntH"
                     /locus_tag="N7A07_000293"
     CDS             complement(13031..14269)
                     /gene="mntH"
                     /locus_tag="N7A07_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU2475043.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14605..15807
                     /gene="nupC"
                     /locus_tag="N7A07_000294"
     CDS             14605..15807
                     /gene="nupC"
                     /locus_tag="N7A07_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU2475044.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15857..18046)
                     /gene="pdeA"
                     /locus_tag="N7A07_000295"
     CDS             complement(15857..18046)
                     /gene="pdeA"
                     /locus_tag="N7A07_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU2475045.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18254..18329)
                     /locus_tag="N7A07_000296"
     tRNA            complement(18254..18329)
                     /locus_tag="N7A07_000296"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18294..18296),aa:Ala,seq:ggc)
     gene            complement(18369..18444)
                     /locus_tag="N7A07_000297"
     tRNA            complement(18369..18444)
                     /locus_tag="N7A07_000297"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18409..18411),aa:Ala,seq:ggc)
     gene            18665..19024
                     /gene="yfeC"
                     /locus_tag="N7A07_000298"
     CDS             18665..19024
                     /gene="yfeC"
                     /locus_tag="N7A07_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU2475046.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            19026..19418
                     /gene="yfeD"
                     /locus_tag="N7A07_000299"
     CDS             19026..19418
                     /gene="yfeD"
                     /locus_tag="N7A07_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU2475047.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19458..20666)
                     /locus_tag="N7A07_000300"
     CDS             complement(19458..20666)
                     /locus_tag="N7A07_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU2475048.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20674..21099
                     /gene="tnpA"
                     /locus_tag="N7A07_000301"
                     /pseudo
     CDS             20674..21099
                     /gene="tnpA"
                     /locus_tag="N7A07_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21217..22632)
                     /gene="gltX"
                     /locus_tag="N7A07_000302"
     CDS             complement(21217..22632)
                     /gene="gltX"
                     /locus_tag="N7A07_000302"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU2475049.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22891..22966
                     /locus_tag="N7A07_000303"
     tRNA            22891..22966
                     /locus_tag="N7A07_000303"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22924..22926,aa:Val,seq:tac)
     gene            23011..23086
                     /locus_tag="N7A07_000304"
     tRNA            23011..23086
                     /locus_tag="N7A07_000304"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23044..23046,aa:Val,seq:tac)
     gene            23133..23208
                     /locus_tag="N7A07_000305"
     tRNA            23133..23208
                     /locus_tag="N7A07_000305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23166..23168,aa:Val,seq:tac)
     gene            23213..23288
                     /locus_tag="N7A07_000306"
     tRNA            23213..23288
                     /locus_tag="N7A07_000306"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23246..23248,aa:Lys,seq:ttt)
     gene            23408..23749
                     /locus_tag="N7A07_000307"
     CDS             23408..23749
                     /locus_tag="N7A07_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU2475050.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23740..24666)
                     /gene="yfeR"
                     /locus_tag="N7A07_000308"
     CDS             complement(23740..24666)
                     /gene="yfeR"
                     /locus_tag="N7A07_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2475051.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24756..25754
                     /gene="yfeH"
                     /locus_tag="N7A07_000309"
     CDS             24756..25754
                     /gene="yfeH"
                     /locus_tag="N7A07_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU2475052.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25751..25969)
                     /gene="ypeB"
                     /locus_tag="N7A07_000310"
     CDS             complement(25751..25969)
                     /gene="ypeB"
                     /locus_tag="N7A07_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU2475053.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25971..27986)
                     /gene="ligA"
                     /locus_tag="N7A07_000311"
     CDS             complement(25971..27986)
                     /gene="ligA"
                     /locus_tag="N7A07_000311"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU2475054.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(28057..29055)
                     /gene="zipA"
                     /locus_tag="N7A07_000312"
     CDS             complement(28057..29055)
                     /gene="zipA"
                     /locus_tag="N7A07_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU2475055.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29285..30046
                     /gene="cysZ"
                     /locus_tag="N7A07_000313"
     CDS             29285..30046
                     /gene="cysZ"
                     /locus_tag="N7A07_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU2475056.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30231..31202
                     /gene="cysK"
                     /locus_tag="N7A07_000314"
     CDS             30231..31202
                     /gene="cysK"
                     /locus_tag="N7A07_000314"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU2475057.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31586..31843
                     /gene="ptsH"
                     /locus_tag="N7A07_000315"
     CDS             31586..31843
                     /gene="ptsH"
                     /locus_tag="N7A07_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU2475058.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31888..33615
                     /gene="ptsI"
                     /locus_tag="N7A07_000316"
     CDS             31888..33615
                     /gene="ptsI"
                     /locus_tag="N7A07_000316"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU2475059.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33656..34165
                     /gene="crr"
                     /locus_tag="N7A07_000317"
     CDS             33656..34165
                     /gene="crr"
                     /locus_tag="N7A07_000317"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU2475060.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34208..35059)
                     /gene="pdxK"
                     /locus_tag="N7A07_000318"
                     /gene_synonym="thiJ"
     CDS             complement(34208..35059)
                     /gene="pdxK"
                     /locus_tag="N7A07_000318"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU2475061.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35164..35531
                     /gene="yfeK"
                     /locus_tag="N7A07_000319"
                     /pseudo
     CDS             35164..35531
                     /gene="yfeK"
                     /locus_tag="N7A07_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35534..36445)
                     /gene="cysM"
                     /locus_tag="N7A07_000320"
     CDS             complement(35534..36445)
                     /gene="cysM"
                     /locus_tag="N7A07_000320"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU2475062.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36579..37676)
                     /gene="cysA"
                     /locus_tag="N7A07_000321"
     CDS             complement(36579..37676)
                     /gene="cysA"
                     /locus_tag="N7A07_000321"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU2475063.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37666..38541)
                     /gene="cysW"
                     /locus_tag="N7A07_000322"
     CDS             complement(37666..38541)
                     /gene="cysW"
                     /locus_tag="N7A07_000322"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU2475064.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38541..39374)
                     /gene="cysT"
                     /locus_tag="N7A07_000323"
     CDS             complement(38541..39374)
                     /gene="cysT"
                     /locus_tag="N7A07_000323"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU2475065.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39374..40390)
                     /gene="cysP"
                     /locus_tag="N7A07_000324"
     CDS             complement(39374..40390)
                     /gene="cysP"
                     /locus_tag="N7A07_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU2475066.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40548..41339)
                     /gene="ucpA"
                     /locus_tag="N7A07_000325"
     CDS             complement(40548..41339)
                     /gene="ucpA"
                     /locus_tag="N7A07_000325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU2475067.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41468..42325)
                     /gene="murR"
                     /locus_tag="N7A07_000326"
     CDS             complement(41468..42325)
                     /gene="murR"
                     /locus_tag="N7A07_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU2475068.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42489..43385
                     /gene="murQ"
                     /locus_tag="N7A07_000327"
     CDS             42489..43385
                     /gene="murQ"
                     /locus_tag="N7A07_000327"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU2475069.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43389..44813
                     /gene="murP"
                     /locus_tag="N7A07_000328"
     CDS             43389..44813
                     /gene="murP"
                     /locus_tag="N7A07_000328"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU2475070.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44818..46122
                     /gene="pbp4b"
                     /locus_tag="N7A07_000329"
     CDS             44818..46122
                     /gene="pbp4b"
                     /locus_tag="N7A07_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU2475071.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46180..47079)
                     /gene="yfeX"
                     /locus_tag="N7A07_000330"
     CDS             complement(46180..47079)
                     /gene="yfeX"
                     /locus_tag="N7A07_000330"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU2475072.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47175..47750)
                     /gene="yfeY"
                     /locus_tag="N7A07_000331"
     CDS             complement(47175..47750)
                     /gene="yfeY"
                     /locus_tag="N7A07_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU2475073.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47811..48260)
                     /gene="yfeZ"
                     /locus_tag="N7A07_000332"
     CDS             complement(47811..48260)
                     /gene="yfeZ"
                     /locus_tag="N7A07_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU2475074.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48247..48672)
                     /gene="ypeA"
                     /locus_tag="N7A07_000333"
     CDS             complement(48247..48672)
                     /gene="ypeA"
                     /locus_tag="N7A07_000333"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU2475075.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48886..49755
                     /gene="amiA"
                     /locus_tag="N7A07_000334"
     CDS             48886..49755
                     /gene="amiA"
                     /locus_tag="N7A07_000334"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU2475076.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49759..50658
                     /gene="hemF"
                     /locus_tag="N7A07_000335"
     CDS             49759..50658
                     /gene="hemF"
                     /locus_tag="N7A07_000335"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU2475077.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50664..51716)
                     /gene="eutR"
                     /locus_tag="N7A07_000336"
     CDS             complement(50664..51716)
                     /gene="eutR"
                     /locus_tag="N7A07_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU2475078.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51762..52262)
                     /gene="eutK"
                     /locus_tag="N7A07_000337"
     CDS             complement(51762..52262)
                     /gene="eutK"
                     /locus_tag="N7A07_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU2475079.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52275..52934)
                     /gene="eutL"
                     /locus_tag="N7A07_000338"
     CDS             complement(52275..52934)
                     /gene="eutL"
                     /locus_tag="N7A07_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU2475080.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52944..53831)
                     /gene="eutC"
                     /locus_tag="N7A07_000339"
     CDS             complement(52944..53831)
                     /gene="eutC"
                     /locus_tag="N7A07_000339"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU2475081.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53852..55213)
                     /gene="eutB"
                     /locus_tag="N7A07_000340"
     CDS             complement(53852..55213)
                     /gene="eutB"
                     /locus_tag="N7A07_000340"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU2475082.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     CIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55225..56628)
                     /gene="eutA"
                     /locus_tag="N7A07_000341"
     CDS             complement(55225..56628)
                     /gene="eutA"
                     /locus_tag="N7A07_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU2475083.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56625..57851)
                     /gene="eutH"
                     /locus_tag="N7A07_000342"
     CDS             complement(56625..57851)
                     /gene="eutH"
                     /locus_tag="N7A07_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU2475084.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57951..59138)
                     /gene="eutG"
                     /locus_tag="N7A07_000343"
     CDS             complement(57951..59138)
                     /gene="eutG"
                     /locus_tag="N7A07_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU2475085.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(59128..59964)
                     /gene="eutJ"
                     /locus_tag="N7A07_000344"
     CDS             complement(59128..59964)
                     /gene="eutJ"
                     /locus_tag="N7A07_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU2475086.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59975..61378)
                     /gene="eutE"
                     /locus_tag="N7A07_000345"
     CDS             complement(59975..61378)
                     /gene="eutE"
                     /locus_tag="N7A07_000345"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU2475087.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAVDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(61390..61677)
                     /gene="eutN"
                     /locus_tag="N7A07_000346"
     CDS             complement(61390..61677)
                     /gene="eutN"
                     /locus_tag="N7A07_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU2475088.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(61784..62077)
                     /gene="eutM"
                     /locus_tag="N7A07_000347"
     CDS             complement(61784..62077)
                     /gene="eutM"
                     /locus_tag="N7A07_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU2475089.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(62116..63132)
                     /gene="pta"
                     /locus_tag="N7A07_000348"
     CDS             complement(62116..63132)
                     /gene="pta"
                     /locus_tag="N7A07_000348"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU2475090.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(63129..63860)
                     /gene="eutT"
                     /locus_tag="N7A07_000349"
     CDS             complement(63129..63860)
                     /gene="eutT"
                     /locus_tag="N7A07_000349"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU2475091.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(63857..64558)
                     /gene="eutQ"
                     /locus_tag="N7A07_000350"
     CDS             complement(63857..64558)
                     /gene="eutQ"
                     /locus_tag="N7A07_000350"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU2475092.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(64533..65012)
                     /gene="eutP"
                     /locus_tag="N7A07_000351"
     CDS             complement(64533..65012)
                     /gene="eutP"
                     /locus_tag="N7A07_000351"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU2475093.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(65025..65360)
                     /gene="eutS"
                     /locus_tag="N7A07_000352"
     CDS             complement(65025..65360)
                     /gene="eutS"
                     /locus_tag="N7A07_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU2475094.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        15396 a        18035 c        16549 g        15405 t
ORIGIN      
        1 ctttaaaaac aggaaattta tctgcgctaa caagaaaatt accactcatt ttattaacat
       61 aataatttca ataaatttac taaccagaag tgcattatca agagatgcat tgctaataca
      121 ccatttatct atcaatcccc ttttaaaatt gtggtttttc ctcaaaaacg gcctgctggt
      181 catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc acccgtgcac
      241 gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct tatctgcctg
      301 ttctttctca tccgtatccg cctgtttcgc gaactgttgc acaagtcggc acactcaccg
      361 aaagagttgc tcgccgttac cgccattttc tcgctgttcg ccctcttcag cacctggtcc
      421 ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat gtccggcggg
      481 attctgtttg gcccgtgggt gggcatcatt accggcgtga ttgcgggtat tcatcgttat
      541 ttaattgata tcggtggcgt gacggcgatc ccctgcttta tcaccagcat tctggctggt
      601 tgtatatcgg gctggatcaa cctgaaaatc cccaaagcac agcgctggcg cgtcggtatt
      661 ctcggcggca tgttgtgtga aacgttgacc atgattctgg tgattgtctg ggcaccaact
      721 accgcgttgg ggatcgatat cgtctctaaa atcgggattc caatgatcct cggcagcgtc
      781 tgtatcggct ttattgtgct tctagtgcaa agcgttgagg gcgaaaaaga ggccagcgcc
      841 gcgcggcagg ccaagttggc gctggatatc gccaacaaaa cgctacctct gtttcgccat
      901 gtcaatagcg agtcattacg caaagtttgt gaaattattc gcgatgacat tcacgccgat
      961 gcggtggcga ttaccaatac cgatcatgtg ctggcctatg ttggtgtggg tgaacataac
     1021 tatcagaatg gcgatgactt cattagcccg actacccgtc aggcgatgaa ttacggaaaa
     1081 atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc catgctggtg
     1141 atcccattgt gggagaaagg cgttgtgacc ggaacgctga aaatttacta ctgccacgcg
     1201 catcagataa cctcgtcatt acaggaaatg gcggttggtc tgtcgcaaat catctccacg
     1261 caactggagg tttcacgcgc cgagcagcta cgtgaaatgg caaataaggc agagcttcgc
     1321 gccctgcaaa gcaaaattaa tccccatttc ctgtttaacg ctttgaacgc tatttcatcg
     1381 tcgatccgtc tgaatccgga taccgctcgc cagctgatct ttaatctgtc gcgttatctg
     1441 cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga gctgtatcaa
     1501 attaaagact atattgccat tgagcaggcc cgctttggcg acaagctgac ggttatctat
     1561 gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc gttggtggag
     1621 aatgccattg tccacggtat ccagccttgc aaaggtaaag gcgttgtcac catcagcgtt
     1681 gcagagtgcg gcaatcgggt acgcattgcg gtgcgggata ccgggcacgg catcgatcca
     1741 aaggtgattg agcgggtcga agcgaatgaa atgccgggca ataaaattgg cctgctgaat
     1801 gtccatcatc gcgtgaagtt attgtatggc gaggggctgc atatccgccg cctggaacca
     1861 ggaacggaaa ttgcgtttta cattcctaac caacgcaccc cagtcgcctc acaggctacg
     1921 ttattgcttt gagccggagt gatattgtga aagtcatcat tgttgaagac gaattcctgg
     1981 cacaacagga actgagctgg ctaattaaag agcacagcca gatggagatt gtcggcacct
     2041 ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc
     2101 tggatatcaa tattccgtcg ctggatggtg tgttgctggc acaaaacatc agccagttcg
     2161 cccataaacc gtttattgtg ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg
     2221 aactggaggc gtttgactac attctaaaac cgtatcagga gtcacgcatt accgggatgc
     2281 tgcaaaaact ggaagcagcc tggcaacaac agcagaccag cagcacgcct gccgcgacgg
     2341 taacgcgtga gaatgacacc attaatctgg tgaaagatga gcgaataatc gtcacgccaa
     2401 ttaacgatat ctattacgcc gaagcgcacg agaaaatgac ctttgtttat acgcggcgtg
     2461 aatcctacgt aatgccgatg aacattaccg aattttgcag caaactgccg ccgtcgcatt
     2521 ttttccgctg ccatcgctca ttttgtgtca atctgaacaa aatccgcgaa atcgaaccgt
     2581 ggtttaataa cacctatatt ctgcggctga aagatctgga ttttgaagtg ccggtcagcc
     2641 gcagcaaagt taaagaattc cgccagttaa tgcatcttta atcgggaact ttcatgaaag
     2701 caccaggctt gcccgccgat caacaatttt tcgccgatct gttcagcggc ctggtgctta
     2761 acccgcaact actggggcgc gtctggtttg ccagccagcc tgcctcgttg ccggtgggca
     2821 gtttatgtat tgattttccc cgtctggata tcgtgctgcg cggcgaatac ggcaatctgc
     2881 tggaagcaaa gcagcaacgt ttggtggaag gagaaatgct gtttattccg gcgcgcgcgg
     2941 ctaatttacc ggtcaacaac aaaccggtga tgctgttaag cctggtgttc gctccgacct
     3001 ggcttgggtt atcgttttac gatagccgca ccacgtcatt gttgcatcct gctcgccaga
     3061 tccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc
     3121 ttagccgttc gccgctggag caaaatatca ttcagccgct ggtgttaagt ttgctgcatc
     3181 tttgccgtaa cgtggtgaat atgccgccgg gcaattcgca gccgcgcggc gattttctct
     3241 atcacagcat ttgtaactgg gttcaggata attatgccca gccgctcacc cgcgagagcg
     3301 tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa actgtttgct cagcatggaa
     3361 cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc
     3421 agaaatatca tttgtcaatt catgaagtgg cacagcgttg cggttttccg gatagcgact
     3481 atttttgtcg cgttttccgg cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt
     3541 ttcagggcta acgtcagaag gttaattctg tttccagcag cgtcaggata ctttgtgcat
     3601 cttgcgcggc aaacaacgac tggcggaagt ttttattcac cagtttacgc gccagctgcg
     3661 agaagacttt cacatgattc atcccttcgt tagcacccag cgtcagcatg atcaccagtt
     3721 cgacttcgcc catttctgac tgccagtcaa ccggtttcgc cagccgggca atgctgatac
     3781 tggaatgacg gatccactga gatttggtgt gcgggatcgc cacgccaaaa ccaacgccag
     3841 tggtgacaat ctcttcccgc tgccagacat cctcttccag ctcgaacgga tgttcagtgc
     3901 gcccgttaac gccgaggtta ccgcacagga actggatcgc ctgctcttta ttgctaaagg
     3961 cctgatcaac gaagatattc tccagcgcca gcagcgggcg aacgtcttct tccggcgtaa
     4021 aggcggtgag taacgcttca atttcctgcg cgctgcgaca ttcacacgcc tgacgtgcca
     4081 gttcccgaca cgcctcgcta tccagttgac gaagctggct tttcaccgcc ggaatgcgcg
     4141 ggctactcat actcagctca tccaggccca gcccaagcag tagcggcaga taacggcttt
     4201 caccgcccag ttcaccgcaa atgcctaccc atttgccccg ctgatgcgca gtggtaacta
     4261 tttgctgcaa catgcgcagg aacgatggcg taatcgggtt atatagcggc gatacgcgcg
     4321 ggttattacg atcgaccgca tacagatact gggtcatatc gttggagccg atactgaaga
     4381 aatcgacctc atcgcagaag tgatcgatga tgtagcacac cgacggcact tccaccatga
     4441 tacccagcgt aatcgtctct gcatgacgca ggccatcgcg cttaagctca acgatcgctt
     4501 tttggatctc gcccttcacc cataagatct gatcgaggcc gtggaccatc gggatcatca
     4561 actgggcgtt gccgaaactg gcggcgcgca aaatggcccg cagttgagtg cggaacaggc
     4621 cagcaaattc cgggtaaata cgtaccgcgc gatagccgag gaacgggttc tcttcctggg
     4681 gaatattcag ataaggaatg cttttatcgc ctccgatgtc cattgtgcgg aagataatcg
     4741 gcttgtcgcc cgctgccagt agcacctgct ggtaggcttc aaattgctcc tgctcgtccg
     4801 gctcgctgtc gcgatccata tagagcattt cggtacggaa cagcccgacg ccttccgcgc
     4861 cgttggcaaa cgcgccaggc gcttccagag cggtgccaat attcgccgcg atatcaatac
     4921 gcttgttatc acgggaataa gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt
     4981 tatccgccag cgtctgcgcg acctgataat aaccgctcac cgcgtcattc gggttaatcg
     5041 ccagcacgcc gcactgggcg tcaagcactg caggttgccc ggcataacgc gcgatcgcat
     5101 ccagcggcag gccgctcagt accgggatcg ccgaggcgcg ggccagaatc agggtatgcg
     5161 aggtgcgccc ggttttctcc agaatcatgc ccgcaagatt ttttaaatcc aggcttaaaa
     5221 actggcttgg ggttaaatcc tcggccacca gaatagtcgg tttttccagc accagattgt
     5281 tgcgcggctt cagttccggc caggtaatat gcagcaactg ttcgctaata tcgcgaatgt
     5341 cgctaacacg ttcgcgcaga taatcgctgg cagaagcaga aagtttggcg caaacctgct
     5401 ccatatttct gatgatcgcc gcccccagcc cctgatgctg ttctgtcatc aggcgacgga
     5461 tattgcctgc aaattcatca tcctgaatca gcgacaaatg ggcgctgagg atagttttgc
     5521 tttcgccgtc acgctcacgc aattgctggt tcagttgctc ggcaagcgtt gccaggctgt
     5581 gctccagccg ggtggaatct tgcgcactgg cggggattgc ccgataactg tcgaggctgt
     5641 cgctttgtaa cagggtcagc gtacccacgc cgacgccgct tgccagcaca ttgccgtaca
     5701 gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg atgcgccgtc agttctgcct
     5761 gcgtaggctg aacgctgtcg ctgtcgataa agcgcacctg gatgtactct tccagcacgc
     5821 gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct gcaactgtca ttaaataggg
     5881 tgccggtgcc aatcagcgcc agcgagcttt tggcatcggc ctttgcgttc tggcgatggt
     5941 taataaaagt gatttcgctt tgccactggc tgcactgttc tttaagttcc cacgccggac
     6001 gagcatgtag accgttaggc aaaggacaga gaaattgaat cgttaacata accgctccgg
     6061 gttaatcagg agatcatctg aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt
     6121 gaataagggc agataaaagt tgctccatct gcaaaatgtc acggcaatcg gcaatcgacg
     6181 cggcgcaatg tccgtggcgg gtggcaggcc ccatcaccac tgtgggtacg ccagtaccgg
     6241 ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa catatccgcc tgtaacggca
     6301 cgccaatttc tgccgccacg gtttcgatcc aggcggtgag ttttggcggc gcaatcagtg
     6361 acttgtcgct taacaccagc atcgggccgt taccaatctg gcgatggtta gccgcgccat
     6421 aattaaagtt tttcgcccag caggcggtat caaggacaat ggcgacgtcc ggcgacaccg
     6481 cgcgggtggc agtttgcccg ccgcgtaacc ccacctcttc gctggaactg gccaccagcc
     6541 acacttccgc aggcagttca gcgctgtgta gttcgcgcag taacgtcacc agcaggtagc
     6601 aaccgaggcg gtcatcaaag gctttcccca tcacccgctg gtgggggaga acctgaaaag
     6661 tggtatcgaa cgtgacgcga tcgcctggac gaattcccgc ctgcatcact tcgtcatacg
     6721 agcgcgcgcc aatatccacg cgcatggcgc tgacatcatt cccctgccgg tcgccgtcaa
     6781 gcaggcctgg aattttgcac tcttcacggg tggtgatgcg caccggctgc agctggcggg
     6841 cagccatgcg tacattgcca accggcagca catcaatcgc cccttcgcgg gagatgctgc
     6901 gcaccataaa tcccacttcg tccatatgcg cacagatcat caccttcgga cctgtcgatt
     6961 cattgaggcg gatcagcacc gatcccagcc catcaaatcg cacttctttt tgcaggcgat
     7021 ccgcttcttc cagcaggatc tgccgcactt cctgttccga ggaggcgatc gcatctgcct
     7081 cgctcaacgc tttcaatagc gataaatcca ttatgcctct cccgtgagca acactgtttt
     7141 cggcatggcg tagagcactt ctgcgccttg cggggtgacc agcacaacat cctcgatgcg
     7201 cacgccccct tgccctggca aataaatccc cggctccacg gtcagtaaca tgcctggctg
     7261 tagcgtcgtg gtgtcccgcg gtgaaaaacg cggatcttca tgaacttcaa tgccgatagc
     7321 atgagcggtg ttatgaccga aatagtggct aaaacctgcc tcggtaatca cccgacgcgc
     7381 ggcttcgtca acctgctggc agcgcacgcc ggggcgaatt gcagagattg ctgcgagctg
     7441 tgcctgcaaa acaatctgat agacgttaaa cagcgggtga gattcggcgc tcaccccttc
     7501 gccattcacc agcaaggtgc gcgtcatatc agagcagtag ccctgataga gcgcaccgaa
     7561 atcgagagtg acaaactcgc ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag
     7621 cgccccacgc cagccactgg cgacaatggt gtcaaaagag gttttttctg cgccctgctg
     7681 gcgcataaac cactccagtt cagcggctat ctcgcgctcg ctcatccccg cctgaataaa
     7741 gcggcgaata tgctctgcac cgcgatcggc aatcccacag gcaaggcgga ttttctccac
     7801 ctcctctggc gttttgattt gccgcagcac atccggcgtg gcgctaacca gtttcgcatt
     7861 gagttcagac tgccagcgat gcgcggtttc ccaactcacc tgctggcctt caaaaccgag
     7921 cgtttgcaac tgctcatcgg caatgatttg cctggcgatg gttgtaagcg tgtgcgtcgc
     7981 gtcaagcaaa tgcagctggt aaccttgcgt gcgggcttct acatccgcgt aatagcgcga
     8041 gtccaccaga atgtgcgcac tttcacggct aatcagcaca tagcctgatc cggtggagat
     8101 ccccagatgc ggctgtttgt tctgccgtga ggagagaagc accgcatcca gttgttgcgc
     8161 cttaagccag tcgcgcagcg aagcgagtaa tgtcatctgg attttccttc ttacaggcta
     8221 tcgattaaca atttgccttt acggaacatc atcagacgca ggaacaccac catcagcgca
     8281 gttatgactg cccccagcgc gatccccgcc atatagacgc ccaggttagt gaccagcggc
     8341 catgcccaga tagcggattc cgggaaccat tgcactgcgc ccagccagac agcggcggtt
     8401 gagccgacaa tcgcgccgac catatacgac ggaatggcgg tgatggggct ttccagcgca
     8461 aacggaatcg caccctcact gatccccata aaggcaagga acatcgcggt cttaccctgc
     8521 ggataaagtt gtgcgttgaa caggcgtttg ccggttaaac gacggtcaat aatggtcgcc
     8581 agacccagac caatcggcgg aataacgata gcgatagaac gagcggtgac cggtaatacg
     8641 tggtcagtgg taaagctgaa ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg
     8701 tcgattgctg tcgccgcggc gatccccatt gcgtacatca gcgcgccctt ctcacctgcg
     8761 gcagtcagca cagtacggat accgccgttg atccagcccc caaagggggt gatgacgtag
     8821 tacatcgcca gcatgacaaa aatagcggaa agaatcggta gcaaaaatgt ggttttgaac
     8881 gctaacagga aatccggtaa ctggattttc tggttcatcc atttcacaag gtaaccggca
     8941 acgatagaaa taatcagcgc accaatgaag gtggacggca ccggcgaaga ggtcgcccat
     9001 tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct gggtagacat caagccgccg
     9061 ataaaaccag ccggaaacgc cagtttgcca ccgatagagt tcgccacaaa agcagcaaac
     9121 atcggaatgg caaaaccaaa caacacgccg ccgaaggact gcgacaacca ggcaaatttc
     9181 agtaaggaga ggtcgaaacc ggagaatttc ccgctattaa gagcatccat gatgccgatc
     9241 tcagcaggaa ttttaagcca gctataagca atcaactggc tgaaagcgag gatcacaccg
     9301 cccataatta aggtcggcac catgcgtgaa atccctgaca tcacatgctg cgggagttca
     9361 ccccagaagc tgcttttaga ggcctgcgga ttcttaactg ccgctgccgc accggatgcg
     9421 ccaggcacaa cggttgcact gcgtttttta atggccataa caattatccc tgtcgaatat
     9481 tactgttgtt cagaggcaat catctcttcg atttctttga tgatgcccgc agcgttttta
     9541 attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc
     9601 ggggtaactg ccacggagtg gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg
     9661 cgattctgga taccgtccgc gccctgggtt tcaattttca cttcataacc ggcttctacc
     9721 gccgcttctt ccagcgcctg agcggccata aaagtgtgag ccaggcccat tgggcaggca
     9781 cataaggcaa tcagtttctt actcatcgtt aattcctcgt tcagtgatag ttgacgagtg
     9841 aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa ataatgcatt tactggagaa
     9901 ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg tgaggggaca caaagaaaag
     9961 ttgaattaac tgtatgattt aaaagattat cgggagagtt acctcccgat ataacaggaa
    10021 ggatttacag aatgtgacct aaggtctggc gtaaatgtgc accggaaccg agaagacccg
    10081 gattgtcatg gacgatgaga tacaccggaa tatcatggac atattcttta aagcgccctt
    10141 tatcttcaaa tgcggcacgg aaaccagagg ctttgaagaa ctcaaggaag cgcggcacga
    10201 taccgcccgc aataaacacg ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa
    10261 aacggcccat aatgacgcaa aacagcgaca acgcacggcg gcaatcggtg cagctgtcag
    10321 ccagcgcgcg ttcggtaata tcttttggct tgagattttc tggcaggcgg ttgtcggctt
    10381 taacaattgc gcgatacaaa ttcaccagtc ctgggccaga aagcacgcgt tccgccgaaa
    10441 catgaccaat ttccgcacgc aatatttcga ggataatggc ctcttcttca ctattcggcg
    10501 caaaatcaac gtgaccgcct tcgcccggca agcttaccca acgcttatcg acatggacca
    10561 gatgcgcaac cccaagcccc gttccggcac cgtaaaccgc aataggctta ccttctaccg
    10621 gttctgcgcc accaaactga atcagatgct cttttttcag catcgggatc gccatcgata
    10681 cagcggtaaa atcgttaata atttccagat ggctaaaacc gagattcttt ttcatttcgg
    10741 caattgagaa cgcccaggta tggttggtca ttgccaccca gtctccggta atcgggcaag
    10801 cgatggcaat acagccgtct ttcacctcga ccttatgttc ttcaagataa acgcgaatga
    10861 ccgcttcgag gctggggtaa tcaagccctg aataggtctt agcctgcgag atttcaccac
    10921 tggcaatatc acacagagca agacgtgcgt tggtgccgcc cacatcaccg actaatgcat
    10981 actttgtcat tcttcaactg ctccgctaaa gtcaaaataa ttctttctca cactgtaaat
    11041 acctgggggc ataacaacaa cgccggaaag gcaggctccc tgtaaatatc gatctgggtc
    11101 acacaattac tttatcgttt cagcaccaat tgcagcgatg cctttttgca agctgggcaa
    11161 actaagtatc tgaccccgca taaggaatag aacatgctcc atccgcgagc cagaaccatg
    11221 ttgttattat cgctccccgc cgtggcaatt gggattgcgt ccagtcttat tctgattgtg
    11281 gtgatgaaaa tcgcctcggt attacagaat ttgctctggc aacgactgcc gggaactctg
    11341 gggatagccc aggattcacc cctctggatc atcggtgtat taacgctaac gggtattgcg
    11401 gtggggttgg ttatccgttt cagccagggt catgccggac cagaccccgc ctgtgaaccg
    11461 ctgatcggcg caccggttcc gccctctgcg ctaccaggac ttatcgtagc attaattctc
    11521 ggtcttgctg gcggcgtcag cctggggccg gaacatccga tcatgaccgt caatatcgcc
    11581 cttgcggttg ccattggcgc tcgtctgtta ccgcgcgtca accgaatgga gtggactatt
    11641 ttagcctctg ccggaaccat cggcgcgctg tttggtacac ctgttgcggc ggcgttgata
    11701 ttttcgcaaa ccttaaatgg cagtagtgaa gttccgctat gggatcgtct ttttgcgccg
    11761 ttaatggcgg cagcagctgg tgcacttact accggattat ttttccatcc ccatttttca
    11821 ctgcccattg cgcattacgg acagatggaa atgaccgata ttctcagcgg tgcaattgtc
    11881 gcggcgattg ccatcgcagc agggatggtc gccgtatggt gcttaccacg gttgcacgcg
    11941 atgatgcatc aaatgaaaaa tccggtgctc gtgctgggta ttggcggatt tattctcggt
    12001 attctggggg ttattggtgg accagtttcg ctgtttaaag ggctggatga gatgcagcag
    12061 atggtggcaa atcaggcttt cagcaccagc gattactttt tgctggcggt aattaaactt
    12121 gccgccctgg tcgttgctgc cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg
    12181 tttgtcggcg tggcattagg gttgatgctg catgagcacg ttcccgccgt accagcggca
    12241 ataaccgttt cctgcgctat tctcggcatc gtgctggtgg taacacgcga tggctggtta
    12301 agtcttttta tggcggcagt cgttgtaccc aataccacat tgctaccgct gctctgtatc
    12361 gtcatgcttc cggcatggct gttattagca ggtaagccga tgatgatggt caatcgtccg
    12421 aagcaacagc caccccacga taacgtttag caaaaaatgc tctcctttat gatttaagag
    12481 ttatggcgat gatacggggc tttacctccc cgtaatattg cgttaacagg ccgctgacgg
    12541 cttcgatcgt gaaggagaat aacaatgttc aggtcactgt ttctggcggc cgccctgatg
    12601 gcatttaccc cgcttgcagc aaacgcaggc gaaatcaccc tactgccatc aatcaaatta
    12661 caaattggcg atcgcgatca ttacggtaat tactgggacg gtggtcactg gcgcgaccgt
    12721 gactactggc atcgcaatta tgaatggcgt aaaaaccgct ggtggcgtca tgataatggc
    12781 taccatcgtg gctgggataa gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc
    12841 gatcgtgacg atcatcgcgg aaaaggccgc ggacatggac accgccatta aacacattcg
    12901 ccaatggagc acaaggcctg atgcgacgct agtgcgtctt atcaggccta caaaccggct
    12961 ctttaacgcg tagggcggat aaggcgttta cgccgcatcg ccaatagtgc cagatgcgac
    13021 gctcattcaa ctacaatccc agcgccgtcc ccaccaataa ccagatattc agcgccacga
    13081 ccagcactac aatcacccag cctgtctgtt ttacgcgttt gctgttcacc agatcgccca
    13141 tcaacttgct gtcactggtg aaaatcagca gtggaaccag cgccagggcg ataccaaaac
    13201 ttaacagcac ctgactcata accagaatcc gtgtcggatc taatcccatc agaatgacaa
    13261 taaatgacgg cagcatggtg actgtacgac gcacccacag cgggatatgg aagcgaatga
    13321 acccctgcat caccacctgc cctgccagtg tccccaccac cgttgaggac agtccggcag
    13381 caaccagact taacccaaag accgttgccg cagcatggct taacagcggt tgcagcgtca
    13441 aataagcctc atcaagatcg gcaacaccag tatgaccaga aaagtggaac gccgccgcag
    13501 ctgtagccat catcgccaga ttgacaaaac cggcaatagt catggcgata gccacatccc
    13561 atttggtggc ggaataacgt tgttgacgcg aaccgccatg taaatgctga gtgagcgaag
    13621 agtgcaaata aatcacatgc ggcataatcg tcgcccctaa cacgcctgct gccaggaaga
    13681 ccgcctccga agtaggtaaa ctcgggatca ccattccttt acccagctgc gccaggttag
    13741 gctgggagaa aatcaactcg acaatgtaag ccgcggcaac aaacaacagt aacccgccaa
    13801 tcactttctc cagcggtttt tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga
    13861 tccccgtcag caccgcgccc tgcaacaacg aaacaccaag aatgagttta aaaccgatcg
    13921 ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat aatttctgcc tgaacccaat
    13981 agaaccacac tacgggacgc ggatagtgat cgcgaatctg ctccgccaga tttttaccgg
    14041 tggcaatccc tagtttggca gagaggatct gaatcagcat cgccatcagg ttggcccaaa
    14101 cgacaaccca cagtagctga tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt
    14161 taccgggatc gatataacca atcgccgcaa tgaacgcagg tcccattaat gcgagcctca
    14221 tcttgcgcgc cgcccgtccg ctgctactct caacgcgata gttcgtcatc ttgtgcctct
    14281 aaaacatagc ctttgctatg tttcatgcta tgccaaacga gaatgattat caaattcatt
    14341 taaatgggtt gtggtgattt ctctgataga ccaggattat gactacgaaa agattgatga
    14401 cttgaatgtt atggttatgt ttaatgttag cacatttaca taactttcag cttccataca
    14461 caacatagca gaaatgtatg acagctcact atttttgaag cttgtcacag gacgtcatta
    14521 tagtgtgtgt cagatctcgt tttccttaac catgttacat agaatgtgca cggaaattta
    14581 acctgcctca tatttggagc aaatatggac cgcgtccttc attttgtact ggcacttgcc
    14641 gttgttgcga tcctcgcact gctggtaagc agcgaccgca aaaaaattcg tatccgttat
    14701 gttattcaac tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt
    14761 ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg atttgccaac
    14821 gaagggacta acttcgtctt tggtagcatg aatgatcaag gcctggcatt cttcttcctg
    14881 aaagtgctgt gcccaatcgt ctttatctct gcactgatcg gtattctcca gcacattcgc
    14941 gtgttgccgg tgattatccg cgcaattggt ttcctgctct ccaaagtcaa cggcatgggc
    15001 aaactggaat cctttaacgc cgtcagctcc ctgattctgg gtcagtctga aaactttatt
    15061 gcctataaag atatccttgg caaaatctcc cgtaaccgta tgtacaccat ggcagcaacg
    15121 gcgatgtcca ccgtttctat gtccatcgtt ggtgcataca tgaccatgct ggaaccaaaa
    15181 tacgtcgttg cagcgctggt actgaacatg ttcagcacct ttatcgtgct gtcgctgatc
    15241 aacccttacc gtgttgatgc ctgtgaagaa aacattcaga tgtccaacct gcacgaaggt
    15301 cagagcttct tcgaaatgct gggtgaatac attctggcag gtttcaaagt tgccattatc
    15361 gttgccgcaa tgctgattgg ctttatcgcc ctgatcgccg cgctgaacgc actgtttgct
    15421 accgtgactg gctggattgg ctacagcatc tccttccagg gcatcctggg ctacatcttc
    15481 tatccgattg catgggtgat gggtgttcct tccagtgaag cactgcaagt gggcagtatc
    15541 atggcgacca aactggtttc caacgagttc gttgcgatga tggatcttca gaaaattgcc
    15601 tctacactct ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc cttcgctaac
    15661 ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac
    15721 gtggtttctc gcttcggtct gaagctggtt tacggctcta ccctggtgag tgtgctgtct
    15781 gcgtcaatcg cagcactggt gctgtaagac catacataaa aaagccgggg ataattcccc
    15841 ggctttttta cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa tacccttgca
    15901 gatattgcac cccgagctta tgcaatagcg cctgttgctg ttgcgtctcg acaaactccg
    15961 cgaccacact caatgacttc gctttcgcca gatcggtaat tgatctcaca atcatcgcat
    16021 ccagcgtatt cgtgacaata tctttcacaa agacgccatc aattttgatg atatcagcct
    16081 gcaaacgctt taaccgttcg tagttggcat agccggtgcc aaagtcatca atcgcaatcc
    16141 ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg
    16201 cctgctcctc ggtgatctca agaatgaccg cctgcgggga gatgtgataa cgcttaaata
    16261 gacgaataat ccgcccggca atattctttt gcagcagcgt gagcggcatt aaattaactg
    16321 aaaagcgcgg gccttttttg tcgcaagggt gtgttgccag ccacttcaac aaggattcca
    16381 gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc aataaggggc agaaacttat
    16441 ccggggtcat aatgccgccg tcatatttca gtcgcgcgag gacctcatca taaccttcac
    16501 cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc accctgatct aacgccgtgc
    16561 gaatggtatt cagcaatagc acctgtttag tggtctgccc ggaaaccatc tcctctcggc
    16621 tatccagcgc cagcacatga tgatgtgcgc aggattgctc cgccagccag cttaactgcc
    16681 ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg cccccaggca gcaccatagc
    16741 ccatatccag cccggtattg ttccagtgaa tttgccgact attcaggatg ttaaccatat
    16801 gctggagtcg cccttccgtt tcaggcccgc tcagcactaa cagcagttca ctacccggca
    16861 attgatacaa cttttcgttt tcctgcatca atggcagcag cgtacggtaa attgatcgga
    16921 tacagtgaac acgcatcatc aacccgtaat gacgactcat aaactcaaga ttatcaatgc
    16981 gcaggcagca aaaactcttg cccgcctctt gctccggcgc ttgttccaac gcacgaaagt
    17041 tgggcaggag cgttaacgga tcggtcagcg cctgcaaatg ccagcggcga ttaagccatt
    17101 cactgcgatg ataaatgcgc accatataga gcaggcaaac gctaaaggaa atcagcaccg
    17161 cgagaataaa tgccagcgaa tattcggttt ccaccccttg caaaaagttc tggttgtaat
    17221 tcagcaggca aagcgttgaa acagcccaag tgagatttaa aaacggatag cgaagcttac
    17281 cgaccccgag ggtaaagatg atgaagaaaa cgggtaccag gtaaccggca ataaagtcgt
    17341 tttcataagg tgtgcacaac agaagtagca gcacgcttaa cgctgccagc cagcttaagg
    17401 taaatgcgcg tttctctttg cccaacgacg gagcgatatc cctgcgccac aatatctgcg
    17461 caaagtgggg acttacaatc atgcgagtga gatagtagaa aagcatgttg taaatcagca
    17521 cagcggtgaa aaggcttagc aaatcaacga ccgtgaaaat ggcatccgca tcgccgaaaa
    17581 aggtggatat ctttagcggg aaatcaaaga agctccccac aagatacatg ctgcatttga
    17641 tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg ccagacatat cggctggtca
    17701 atccataacg ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca cacaacacga
    17761 caaacgtctg gctaccgagc atgaccgcag tctgtaaaaa agagagatga aagttccaca
    17821 gattggtgca aaacatcccg cataaaacgg gaacgacacc gcgccagcca aagataaaca
    17881 agattgacag cattacgcac agtggcatcc acgccagaaa gatataactg gaatggataa
    17941 tggcaagtgg cgaaataaaa cgggatagct gaataagtac cacggtgagc gtaaacgcta
    18001 gtgtgaatat cttgatattt tttatcaggt tatgctccac aaacatgagt acactactct
    18061 cactgatcgg ggttttcagg cacacaaata ataagtttat tcgcattgcg aagcaaacag
    18121 gggccatcct gttgccgatt tttcatccaa tgatgctttc accaggcggc actaacccgg
    18181 acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg
    18241 agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc
    18301 aagcgctctc ccagctgagc tatagcccca cgatgcgttt acgtaccaaa tttgctgggt
    18361 gcaaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg
    18421 ctctcccagc tgagctatag ccccgtcacg taaagcttgt cgagttgacg ggcggcatca
    18481 tatgaattcc gcccgaatgt gtcaacggca aattgcaacg tgtagtttca atcgctgaaa
    18541 aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg
    18601 tttcctgtaa agtggtgcta taaaatgaac tactaataga cccacataca ttcagggaat
    18661 tgttatgttc aaggagagga tgacaccaga tgaacttgcc agactgaccg gctatagccg
    18721 ccagaccatt aataaatggg tacgcaagga aggctggaca acgtcaccaa aacctggcgt
    18781 ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgtaa
    18841 tgctgaacgt ccagaaggtc agggagaagc gcctgccctt tctggtgatg cgccgcttga
    18901 agtattgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc
    18961 cttgcttctg cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa
    19021 ataatatgaa aagattacgc aataaaatga ccaccgaaga actggctgaa tgcctcggtg
    19081 tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc
    19141 ccggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgagttta
    19201 ttcagaacac gccagccttc cataacacac cgatgcttct ggaagccgaa gaaccccttg
    19261 ccgaatacgc accggggatt cgcactcctg cttatcggca aatcatcagt gcaatagaca
    19321 acatgactca cagtgagcag gaaaaagttg cgcaattttt gtcacgcgaa ggaattcgta
    19381 atttccttgc tcgtctcgac atagatgaat cagcttgaca tcctccacgc cctgaatgac
    19441 gaggatccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac
    19501 gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg
    19561 ttacgagcgc cgtttacatc ggcgttcgct gtatatccac atacctggca tctgaattta
    19621 ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg
    19681 tacgctggcg gaacagccag tacctgaccg ccacgccaga gctgcttgta ctcaagctgg
    19741 cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga ttttgcccgg
    19801 acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc
    19861 aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac tttgtgaagg
    19921 tagtccctgc ggatatttgc ggtacaggaa tgcagtcgct gtattttgcg tttctgcttc
    19981 tgccagttat tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccaacttc
    20041 ttctggtttt tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgtggcg
    20101 agtttagcca cgccagcatc cagtccgacc attgatgctg aagggtgaac cggagttgat
    20161 acttcacttt ctgtctgaat actgatgtac cacttaccgc aggactggct gacagtgaca
    20221 tttttcacaa cacccgtgac ctgacggctg ttcaggtagc gcatccagcc aagtttcggc
    20281 agaaaaatac ggctgttttc ctgatcgagc ttaacgccct gcgggtagcg gaatgcatca
    20341 ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tccggaagaa gtttttgtaa
    20401 gcccgctcaa gctctttcag tgactgttgc aatggctgtg acggggcatc tttaagccat
    20461 tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc gtaagggatg
    20521 tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt gaaaacgaaa
    20581 cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccacctgg tcttaactgg
    20641 aatttaaatg cctgtagtcg tttcacaggc caaatgatac tttggtctat gaataaagaa
    20701 accgatatcc gacggggaag gcattgtgtt ttcctgatgc atgttcatct gttctttgtc
    20761 accagatacc gacgtcagat ttttgactat gacgcgacag aaaaactacg cacttacttt
    20821 tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa tggatggcga accagatcac
    20881 gtccatttgt taactaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc
    20941 aaaggcgtat cgggtaggtt actgcgacga gatcgaccag atattgcagt caggtattac
    21001 tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcggagg tgcgccaata
    21061 tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc
    21121 ttatatcccc gccctgaagg acggggtttt acggcgcacc ggataaataa aaaacggcag
    21181 gggtatctct cctgccgttt atcttttatg cgctaattac tgctgatttt cgcgttccgc
    21241 aataaaagcc agcgctttgt tgatacgctc gatactgcgg gtcttaccga tcgcgtgaac
    21301 ggtaacgtcc agcgccggag actgccccgc accggttacg gcgacacgca gcggcatacc
    21361 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    21421 ttcagctgtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    21481 cgctaccgga cgcagatgtt ttttcgcggc gtcggcatcg aactcagcaa aatcttcgta
    21541 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    21601 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    21661 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    21721 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    21781 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    21841 cagatagttc agcagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    21901 tgccccgtga cgtttggaca gttttttgcc gtcatcgccg ttgatcatag aaacgtgcgc
    21961 gtaaaccggt accggcgctt tcagggcttt aagaatgttg atctggcgtg gcgtgttgtt
    22021 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    22081 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    22141 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    22201 gttagcaaaa cgtacgacac acggctcatc atcagcgtga tgctcatgac tgtggcggca
    22261 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    22321 gcgctcttta gagcaatagc atttataagc tgtgccctct tccagcatct gatcgatcac
    22381 cgcgttgtag cgatcaaaac gtttggtctg gaagtacgga ccttcatccc actccaggct
    22441 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    22501 ggtgtcttca atacgcagca cgaactcacc gccgtgatta cgtgcaaaaa gccaggagta
    22561 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    22621 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    22681 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggttgc
    22741 aaccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    22801 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    22861 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    22921 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    22981 tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc
    23041 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttt ctcaccagct
    23101 aaatttcttg taaaaatgtg aagtaccgaa gtgggtgatt agctcagctg ggagagcacc
    23161 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact tcgggtcgtt
    23221 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    23281 acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctatctgcga tttgtaagat
    23341 tgatttttct tctcaagttc cttgttcttc tgccgatatt acctcgtaca gtgacgagga
    23401 ctgaacaatg gccactatct cttcaacttc aattccttcc atacaaacac agagtagtaa
    23461 cagggcatca caaggtagcg atgttgcttc acaaatcgcg cggataagtc agcaaatcat
    23521 caaactcact caacacatta aagagattgt agacactagc ggcagtgctg aagacaagca
    23581 aaaacaggcc gaattgattc aacagcaaat caccctactg gaaacacaac tggcgcagtt
    23641 gcagagacag caggcagaaa aggcgcagga gaaagagcag cgtttgtctc tgaatgtatc
    23701 ccttctaaat cctgttgaaa atacgactca tatcggtatc tatatctgat acaacggatc
    23761 cccttcccgc ccagccatta gcgtattgcc ctggtctcgt accacgtccc ataacgcttc
    23821 agcggcggtg gaaagtgaac ggtttttcct acgcaccagc atcaactggc gttccaccac
    23881 cggcgtaatg cgctttacca ccagcgggct accttcaggc aatggtagcg ccagtgcggg
    23941 aagaatactg atgccgatgc ctgcggctac catcgggaac agcgtcgccg gatgtccaat
    24001 ctcctgcaca atattcgcct gaataccatt gcgcgccagc gctgcgtcaa tcagcggtcg
    24061 gctgcctgac gcataatcct gcaacaccaa tttcgctccc tgtaatgcct gccagggtac
    24121 ataatcttcc gcggccaaag cgctatcccg atggcaaagc agaaagaaag gctcggaaag
    24181 aatcacttca cattgcagat cgcccactgg gcctggatcg atgacgatgc caaagtcgac
    24241 atctccctgg cgaatacttt ccatcaccca ctgctgcgga cgatcgtgca ggacaaactg
    24301 aatatctgga tagcggcgat ggctttcagc aatgcattgc ggaataagat gcgcggaaat
    24361 ggtctggctg gcagcgaccc gaacttttcc gctcagttgt tgccccatac gcccggtatc
    24421 gcgcaacgtg ctgttaagtt catccagcag tcgctcaaga cgcaaagcca actgctgccc
    24481 tgcatctgta agcaccactt cacgcgtggt tctgtccagc aggcgaacac cagtatgatt
    24541 ttccagctcc ttcacactgt gacttactgc cgactggctc aggccgatac gctctcctgc
    24601 acgactaaaa cttttctcct gcgctactgt gacgaaaacc tttaattgtt ttaaagaata
    24661 attcatctgt tttcttcatg aatggatgca ataaatcaat tttatttctc aaactggaag
    24721 aagcacaata gaaccatcga tcatctggag tctttatgaa actttttcgt atcctcgatc
    24781 ctttcacctt aaccctgatc acggtggtgt tgctggcctc tttctttcct gccagaggcg
    24841 atttcgtccc cttctttgaa aatctgacca ccgcagctat tgccctgctg ttctttatgc
    24901 acggcgcgaa gttgtcgcgt gaggcgatta ttgctggcgg tggtcactgg cgactgcatt
    24961 tgtgggtaat gtgcagcacc ttcgtgctgt ttccgattct gggtgtactg tttgcctggt
    25021 ggaaaccggt aaatgtcgac ccgatgctct actccggttt tctctacttg tgcattctcc
    25081 cggctaccgt gcagtctgca atcgccttca cgtcaatggc gggcggtaac gtcgcggcgg
    25141 cggtttgttc tgcgtcagca tccagtctgc tggggatttt cctttcacca ttgctggttg
    25201 gtctggtgat gaatgttcac ggtgcagggg gcagccttga gcaggtcggt aaaattatgc
    25261 tgcaactgct gctgccgttt gtgttggggc atctttcccg gccgtggatt ggtgactggg
    25321 tgtcgcgcaa taaaaaatgg attgcgaaaa ctgaccagac gtccattctg ttggtggttt
    25381 atacagcgtt cagcgaagcc gtcgttaatg gtatctggca taaagttggc tggggatcat
    25441 tgctgtttat cgtggtggtc agctgcgttc ttctggctat cgtgattgta gttaacgtct
    25501 ttatggcacg ccgactgggc ttcaataagg cagatgaaat tactatcgtc ttttgtggtt
    25561 cgaaaaagag tctggcaaat ggcatcccga tggcaaacat tctgttcccc acatcggtga
    25621 tcggtatgat ggtgctgccc ctgatgattt tccatcagat ccaattgatg gtctgtgcgg
    25681 tgctggcgcg tcgatacaaa cgccagaccg aacagttaca ggcgcagcag gaaagcagcg
    25741 ccgataaagc ttaaagcgga cgcttcaggg gctggaccag ttgcgtcagc ccctcggttt
    25801 taatcagcag cgtgatttgc attagctcac cgagcttccc tgccgggaac tcatctttgc
    25861 gggcaaacca cagcagatac tcctccggca ggtcgattaa gcgacgccct ttgtatttgc
    25921 caaacggcat tatcgtattg gctatttcaa tcagctgctc tttttccatc tcagctaccc
    25981 agcaaacgca gcatttccgc ttcgtcgatg acttcaatgc ccagttcctg cgccttcgcc
    26041 agtttagatc ctgcagcttc acccgctatc accagatcgg ttttcttcga cacgctgccc
    26101 gcgactttcg cgcccagttc gaccagtcga gctttagcgt catcacgcga catctggctt
    26161 aagctgcccg taagcaccac ggttttacca gcaaacgggc tgtcaatctc ttccgcgttg
    26221 ataacgatcg gcgcaggcca gtgaacacct tccgccaaca gctcgctgat gacattgcgg
    26281 ttgctttctt cggcaaagaa gttgtgaacg tgggatgcaa cgacaatgcc aacatcaggc
    26341 accttttgca gctcttcaat cgaagcggct tccagcgctt ccagcgttcc gaaatatgcc
    26401 gccagacctg ctgcggtggc ctcgccgact tcacggatgc caagtgcata gaggaagcga
    26461 gcaaaggtgg tttctttcgc tttttccagc gcgttaacca cgttttgtgc cgattttggc
    26521 cccatacgct ccagtccggt cagtttgcct gcggtgagtt tgaacagatc cgccggagtg
    26581 tggacatatt ctttttcaac cagctgatcg atgattttgt cgcccattcc gtcaacatcc
    26641 atcgcacggc gggaaacaaa gtgtttcagc gactctttac gctgcgcacc gcaaatcaga
    26701 ccaccagtac agcgggcaac cgcttcacct tccacacgct caacgtcaga accacatacc
    26761 ggacaatacg tcgggaatac aacctcacgg gtatcttccg ggcgttcaga aagcacgacg
    26821 ttaaccacct gcgggatcac gtcgccagcg cggcgaatca ccactttatc gccaatgcgt
    26881 aaaccaagac gttcgatttc atccgcattg tgtaaggttg cgttactcac cagcacgcct
    26941 gcaacatgga caggttccag acgcgcaaca ggcgtaatcg cgccagtacg cccaacctga
    27001 aactcgacgt cacgcacaaa ggtcatctgt tcctgcgccg ggaatttaaa cgctaccgcc
    27061 cagcgcgggg ctcgcgcgac aaagccaagc tgctcctgct gttccagtga gttgacctta
    27121 atcaccacgc cgtcgatatc aaagcccagc gtcgggcggt cttcttccac tttgtgatag
    27181 aacgccagca cttcttccgc cgattcacaa agcgttaccc gatcgctgac cggcaacccc
    27241 cactttttaa attgcagtaa acggccaaga tgagtatccg gcagctcgcc accttccaga
    27301 acaccaacgc catagcagaa aaaagtgagc ggtcgcttcg ctgtaatacg cggatcaagc
    27361 tgacgcagtg aaccagctgc cgcattacgt gggttagcaa acactttccc gcccgtgcgt
    27421 cgcgcatctt cgttaatctt ttcgaacccc gcctgcggca ggaacacttc accacgcact
    27481 tccagacgcg ccgggatatt ctctccgtgc aacttcagtg gaatggcgcg aatagtacgc
    27541 acattagacg tgatatcttc cccggtggtg ccatcgccac gagtcgcggc actgactaaa
    27601 acgccatttt catacagaat actgacggca aggccatcca gcttcagctc acagcaccag
    27661 gtgactttct cgttgctttt cagacggtcc tgcacacgtt tgttgaaggc aagaaagctt
    27721 tcttcatcaa aaacgttatc cagcgacagc attggcactt catggcgaat ctggctgaaa
    27781 gccgccagcg gcgcagcacc tacacgttgg gtaggcgaat caggcgtaat cagttctgga
    27841 tgtttggttt ccagctcgcg cagttcgcgc atcagcctgt cgtattcagc gtcgggaatt
    27901 tccggcgcat ccatcacatg ataaagatat tcatgatggc gaagcgtcgt tcgcagttct
    27961 gtcagttgtt gttcgattga ttccatatcg caccatcaat gctaaaaacc cccgacaagc
    28021 gggggttcga agaggagtta atttgcctta agtgtatcag gcgttggcgt ctttaacttc
    28081 gcggatgatg tcctggtact cgcgcaattt ctgcggagtc atcatacggc gctgatcgtc
    28141 aagcacgaca ccgcccactt catcggcaat atgctgcgca gattgcagca tcagcttgaa
    28201 gttctgcagc tcgtcaccgt aagacggtac ctgcataaag atagtgacac ccggagtagt
    28261 gaaatccttc atttcaggat caaaggttcc cggtttcacc atattcgcca ggctaaataa
    28321 cgccgggccg ctgccatccg ggctaagatg acgatggtaa atattcatat cgccaaaaat
    28381 gaagcccgct tgttgaatgc tgttaagaag cagttcaccg tttagctcgc taccgtgatg
    28441 cgccgcgacg ttcatgataa tcaccgcttc tttgcgcttc ggtttatcca taactggagc
    28501 cggttccgct acaggctcag gctgtggtgc cgctacgggt tctgcaggct ggaaagcctg
    28561 ttgtgccggt tgcggtgctg aatgcactgg ctgcggcgct ggcgcgacct gtggcgaaac
    28621 tggctgctgc aacggctgtt caggctgcgg ctgataggca ggctgctgca cgggttgctg
    28681 caccggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
    28741 gaccggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
    28801 acgagcagcc tcatgctcct gggcgttagc cggggcatga ttcacgcggt gaacacgaac
    28861 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
    28921 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
    28981 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
    29041 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
    29101 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
    29161 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
    29221 cgcaattacc cggtaaaata cgcacaattt tcctgggatt ggtcaaaagg agctcatcct
    29281 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
    29341 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
    29401 tattttgttg atggggggcg cattctggtg gctctttacg caactcgatg tctggatccc
    29461 gactctcatg agttatgttc cggactggtt acaatggctg agctatctgt tgtggcctct
    29521 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
    29581 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
    29641 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
    29701 atggcaaaaa tttgcctggt atctgccacg cgcaattgta ttgctaattc tttacttcat
    29761 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
    29821 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
    29881 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagtctgtt
    29941 tacgatgatc ccgctgctta atctgttcat tatgcccgtt gccgtttgtg gcgcgacggc
    30001 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
    30061 ttttgtaaac cgtttgtgtg aaacaggggg ggcttatgcc gccccttatt ccatcttgca
    30121 tgtcattatt tcccttctgc atatagatat gctaaatcct tacttccgca tattctctga
    30181 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
    30241 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
    30301 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
    30361 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
    30421 aactggttga accaaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
    30481 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
    30541 tgaaagcgtt aggtgcaaac ctggtgctga ccgaaggtgc taaaggcatg aaaggcgcaa
    30601 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
    30661 tcagcaatcc ggcaaaccct gaaattcacg aaaagaccac cggtccggag atctgggaag
    30721 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
    30781 gcgtcagccg ctacattaaa ggcaccaaag gtaagaccga tcttatctct gtcgccgttg
    30841 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
    30901 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
    30961 tcgataaagt cattggcatc accaatgaag aagcgatttc taccgcgcgt cgtctgatgg
    31021 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgctgcg gcgttgaaac
    31081 tacaagaaga tgaaagcttt accaacaaga atattgtggt tattctaccg tcatcgggtg
    31141 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
    31201 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttc aggtgctttt ttgtggcctg
    31261 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
    31321 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
    31381 atgatttggt tcaattcttc ctttagcggc ataatgttta atgacgtacg aaacgtcagc
    31441 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
    31501 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
    31561 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
    31621 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
    31681 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
    31741 tctgggcctg actcaaggta cagttgtgac tatctccgca gaaggcgaag acgagcagaa
    31801 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
    31861 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
    31921 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
    31981 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
    32041 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
    32101 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
    32161 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
    32221 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
    32281 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
    32341 attcaggatg aagtcattct ggttgccgct gacctgacac cgtccgaaac cgcacagctg
    32401 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
    32461 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
    32521 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
    32581 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
    32641 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca agtagaagta
    32701 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
    32761 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
    32821 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
    32881 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
    32941 aacccgttcc tcggctggcg cgctatccgt atcgcaatgg atcgtaaaga gatcctgcgc
    33001 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
    33061 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
    33121 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
    33181 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
    33241 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
    33301 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    33361 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    33421 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    33481 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    33541 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    33601 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    33661 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    33721 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    33781 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    33841 gcctgtagac ggcaccattg gtaaaatctt tgaaaccaac cacgcattct ctatcgaatc
    33901 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    33961 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    34021 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    34081 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtgactg tgggtgaaac
    34141 cccggttatc cgcatcaaga agtaattctt gctgcagtga aaaatggcgc ccatcggcgc
    34201 cattttttta tgcttccgcc agcggcggca aaatcaattc atcgctctca tgctgctggg
    34261 tgtagcgcat cacttccagt acgcgcaacc ccgcgcggtg caccgcatcg tttaatgcct
    34321 tccctttcag caaaccactg ataagctgag cacaaaacag gtcgccagtc cctttcaggt
    34381 cggtttttac ccttgaatgg gaaatgacat tcacgctgtc ggcagtgacc accacaacca
    34441 gcatctcctg attttcttca ttaccggagg cgctggtaat caccacccat tttagtgtgt
    34501 ctgaaagcag actttttgcg gcagcaatgg cactgtcgag atcgcggcaa tttttaccgg
    34561 tcaggatttc caactcaaag atattggggg taattccctg cgccagcggc agtaaatatt
    34621 gtcgatacgc ttcgggaagg tcaggtttga cataaattcc gctgtcaata tcgccaatca
    34681 ccggatcgac catgatcaat aggtcaggat ggtctttgcg tagcgcagtc agccactcgg
    34741 caaggatttt gatttgcgat gccgttccca tatagcccgt ggttacagca cgaagttggc
    34801 gcagcgcatc acgctcctga agtgcacgca aatagccgct aaaccattca tccggaatcg
    34861 caccaccgta gaaagtgtca taatgcggcg tattgctcag caataccgtc ggcacggcaa
    34921 agacattcag gccgttctgt ttgatagcag gcacggcaat gctgttgccc acgctgccgt
    34981 aaaccacctg cgactgcacg gcgacgatat ccgcctgcag cgccctgctc ttatcgttaa
    35041 acaacaacaa actactcatt taattttttc tccttgccga tgatcctcat cgtattccaa
    35101 ccgaaacttt acctgattct ggcagtcaaa tcggctatta caaaacaagg ataaggtaat
    35161 tcagtgaaga aaatcatttg tctggtcatt acactactaa tgacactccc cgcttacgcg
    35221 aagttaactg cccatgaaga agcccgcatc aacgccatgc tggagggatt agcacagaaa
    35281 aaggatttga tatttgtgcg caacggtgat gaacatacct gcgatgaagc ggtttctcat
    35341 ctgcgtctga agctgggcaa tacccgtaac cgcattgaca ctgccgagca gtttattgat
    35401 aaggttgctt cgtcgtcatc gattaccggg aagccgtata ttgtgaagat ccccggtaag
    35461 agcgatgaga atgcacagcc tttttacatg cgttaattgc gcagacggat aaaacggtgc
    35521 ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca aacaccccgg
    35581 tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca ccagggttag
    35641 cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca cagaatattc
    35701 cttcccgcac cgccagttcg cgcatggtgt tttccgcatc gcgctgatga atatccagca
    35761 cctcatctac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa
    35821 tgccgggaat gctactgccc tcttccggct gcaggccaac aatggtcacc ggtttggatt
    35881 gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg ctggagacaa
    35941 aataggtaat gcgcccgcca gtctgctgcc agatttccgg cccggtagtg gtgtaatggg
    36001 cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcacca cgatccgcca
    36061 tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgacc agaatcagtt
    36121 ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca
    36181 atttcatgcg atagccttta agcgcggcaa tcattgccag cgcaatgccg gtgttaccac
    36241 tggtggcttc gattaacaca tcacccggtt taatttcccc gcgcttttcc gcctcgacga
    36301 tcatcgaaag cgccgcacga tcttttaccg aacctgccgg gttattgcct tccagtttta
    36361 accacacttc actgccgtta ttcggcccca ttcgctgcaa cttcaccaga ggcgtattgc
    36421 ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc ctgatgcgac
    36481 gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc cattaaaaag cccggatcgc
    36541 gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca
    36601 agttcctcat ctcggggttc gatacgctcg tcgccgttat acagccgcgc atgttgcaga
    36661 ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc
    36721 ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg
    36781 gcttccagta cctgtaccgg cagcggcgaa tcgaggctgg tacggcggct gatatccact
    36841 tcccacgggc gcaggaagag atccaccggc ccctgatacg caggtgtgta gcccagcggc
    36901 cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag gcggttcact
    36961 tcgcccataa attcgagcac aaaacgggtc gccggttcgc gccatacctg atccggcgcg
    37021 tcagcctgtt caatattgcc ctggctcatc acaactacac gatcagctac ttcggtcgct
    37081 tcttcctgat cgtgggtcac aaaaacgctg gtgaatttta gttcttcatg gagttgacgc
    37141 agccatcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc
    37201 agcagaattt gcggttctac agcaagcgca cgcgccagcg ctacacgctg tttctggccg
    37261 ccggaaagct gcgccggata acgatccgcc agatgggcaa gctggaccat ttccagcaat
    37321 tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc
    37381 aggccaaaag cgatattgtc gaacaccgtc atatggcgga acagcgcgta atgctggaac
    37441 acgaaaccga ctttacgatc acgtgcgtgc aggcggctca cgtcggtgcc gtggaagcga
    37501 atatgcccgc tggtttgatg ctccagccca gcgataatgc gcagcagcgt ggttttcccg
    37561 gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag tgagatatcg
    37621 ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat gctcatgatt
    37681 ttcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc cactgcaaca tactctttaa
    37741 aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg gcggtaaagg agccgactgt
    37801 gttgtagtcc tgctccagca attcaatctg taacggcagc gaaagtgttt cgccacgaat
    37861 cgagccggaa accaccgaca ccgcgccaaa ctcgccaatc gcgcgggcgt tggtcagcac
    37921 tacgccataa agcagcgccc agcggatgtt cggcaatgtg acacggcgga acatctgcca
    37981 gccggatgcg ccaagcaaaa tcgccgcttc gtcttcctga ctgccctggc ttaacatcac
    38041 cggcaccagt tcgcgcacca caaacggaca cgtcacgaag atcgtgacca gcaccattcc
    38101 gggccaggag aacataattt gcagattatg ttcatccagc cagccgccga gcgggccgtt
    38161 ggagccgtag aacagcagat acaccagacc ggcaaccacc ggtgacacgg caaaaggaat
    38221 gtccagtagc gtcagcagta actggcgtcc ggggaagtta aagcgcgtca ccagccaggc
    38281 cagcagaata ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag
    38341 ccagatggcg tgcagcatat ccggatcggc cagattctgt aaaaccggca tcagcccttt
    38401 gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg ccgataccag
    38461 catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt
    38521 caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga
    38581 gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc aatcgcgctc
    38641 gccgccgggt aatcaaactc ctgtaagcga acgaaaatca tcagcgacgt cacttccgtc
    38701 ttccaagcga tgttcccggc gataaatatc accgcgccaa actcaccaag actacgggta
    38761 aacgacaacg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac cactttgcag
    38821 aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc cgggcctaac
    38881 tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa ggccattgcc
    38941 accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag ccattcaccg
    39001 taaaaaccgt ttacagaaaa gagcgaggcc agcgttaaac cggcgactgc cgttggcagc
    39061 gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg ataacgggtt
    39121 aaaatccacg ccatcagcag gccgaaaacg ccgttaaaaa ttgatgccac aaacgccgac
    39181 agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg tgatcacttc ccagtactgc
    39241 gcccagctca tctcagaaag ctgcatcacc agcgcggaga gtggcagcag caatatcagg
    39301 cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcaggac gcgtcttgat
    39361 gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg
    39421 taaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg cggaacagtt
    39481 cggtctgcgg gaatttatct ttcagtttgt ccatgacttc cgggttattc acgcggtagt
    39541 aatagtcggt gatgatggtt tgcgcctgcg ggctgtaaag ccagttcaga taggcttttg
    39601 ctgctttttc ggtaccgttg gcttgcacgt ttttatcgac ccacgccacc gggaattctg
    39661 ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcgtac tgtttgcgga
    39721 tgttgttcac ttccgattca aagctaatca gtacatcgcc caggccgcgc tcggcaaagg
    39781 tggtggtcgc gccacgaccg ccggtatcga acacttcaac gtttttcagg aactgagtca
    39841 taaactgttc ggttttggct ttgtcgccac cgtcagcttt atccgctgcg ccccatgccg
    39901 ccagataggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt
    39961 cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta cggaccagga
    40021 agcccatggt ggagtagaac ggcgagctgt tattcggcag gcgcgactgc cagtccgccg
    40081 ggataagctt gcctttatcg tgcagaattt gtacgtcggt cacctggtta taagtgacaa
    40141 cgtcggcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg gcatgagatt
    40201 gttttatcgt cagtttgtcg ccgccgttat ctttggccca ttgctgttca aacggcgggt
    40261 tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct
    40321 gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt ttcagtaagt
    40381 taacggccat tgcgcaccct tataaattta atgactttct aatagccatc atatttataa
    40441 cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt tgaaaaggga
    40501 ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca gataccgacg
    40561 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    40621 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    40681 cgacgcagcg ggattgcttt tgccatttca gtcagcaccg actctggatc ttccgggttc
    40741 gactggcggg caatgctttc cgccattggt gtgcgaacgt atccagggca tatggcgtta
    40801 acgcgaatac cagactgcgc atactccacc gccagcgatt ttgtcaggcc aacaatcgcc
    40861 gctttcgtta aggcgtacgc cgtttcaccc ggatcggcca ccatatcacc agtgactgaa
    40921 gacatcatca caatgcgacc atctttgcgg gcaatcatct ccggcagcac cgccttcgtg
    40981 acgttccata cgcctttaat attgatgtca atatggaaat cgcgatcgtc atcgctcata
    41041 tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct
    41101 tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    41161 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt ttcgatctca
    41221 ggggagatat ccagcaagat taggtttgcg ccatgacgcg caaaagttct ggcaattcct
    41281 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    41341 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    41401 aacaaaatat tactgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct
    41461 ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgact
    41521 ccacatcatt gagttgcaca agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg
    41581 tgcgggtaga catcgacgag ctacgccatt cggtttcacc ggaaacggta tcgagcgtga
    41641 aatgcgccag ccgccgcagc ggagagtcag ccagagaagt aatggcaatc accgttgccc
    41701 cctgctttcg tgccgcttct gcgcacaaga caatctcttt tttactgccg ctgtaagaga
    41761 tagcgatttg tacatcgcct tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt
    41821 cggcttcgca ggccacgcga tatccaattt tcatcagttt gaacgacaga tcgcacccca
    41881 ccagcgccga tccgcccagg cccgttatct ggataaacgg tgctttactg atgacctcaa
    41941 tgattttttg cagccgcgcg tagtcgaaca acgcacaggt ttgctccagc gccagctctt
    42001 tttcgcgatt caatttgcgg gcaatcactt ccagcgaatc atcactggta atcgaactgt
    42061 gcaggtgctg cgccgtggca ttcgttttct cccggctggc actgtattcg cctattaacg
    42121 ccatacgcaa ttcagtaaac ccttgtgcgc cgagcttctg ggcaaacttc acaatactcg
    42181 actggctgat gccgagctgt ttcgccatct tgcgcgaaga gacggatttc agctcactca
    42241 cgttggcccg cagaaagtca gcaatttttt gctcgttctc ggtaaattca ctttccgcat
    42301 tgcgtatttt cgtcaggtac aacatcttcc actctcctgg gctggcagca aaaattccgc
    42361 ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt cctttaagtc
    42421 tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa atagtaaggt
    42481 caccaccgat gcaacttgaa aagatgatta ccgaaggctc gaacgccgcc tcggctgaaa
    42541 ttgaccgcgt atcaacgctg gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac
    42601 cgcttgccgt tgagcgcgta ctgccggata tcgccgcggc aatcgatgtt atccacgccc
    42661 aggtaagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatccggt cgtctgggga
    42721 ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa accgggtctg gtagttggat
    42781 taattgctgg cggcgaatat gccattcagc acgcggtgga aggcgctgaa gatagccggg
    42841 aaggcggtgt taatgatttg aaaaacatcg gattaaccgc gcaggatgtg gtggttggca
    42901 ttgctgccag cggtcgcacg ccgtatgtga ttgccggact ggaatatgca cgccagctcg
    42961 gctgccgtac agtgggaatt tcctgtaatc cggggagcgc cgtttccacc accgctgagt
    43021 ttgctattac gccggtggtt ggggctgaag tggtcaccgg ttcttcgcgg atgaaagcag
    43081 gcacggcgca gaaactggtg ctcaatatgc tttccaccgg gctgatgatt aaatccggca
    43141 aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc
    43201 aggtcaatat cgttaaaaac gccaccggat gtaatgccga acaagcagaa gcagcgttaa
    43261 ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg
    43321 cagaagctaa aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt ttagacaagg
    43381 aataacccat ggccaaagag atcagcagtg aacttctgaa caccattctt acccgtgtcg
    43441 gcggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctgcgt ctgggtgtac
    43501 atgacagttc actggttgat cccgatatca aaacgctgga aggcgtgaag ggcgtcattt
    43561 tgaccagtga ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag
    43621 cgatgagcga gctgctggga gaagcaccgg tacaggatgc cgcagaaatc gccgcccaga
    43681 acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc gccaaattcg
    43741 ccaccatctt cacgccgctt atccccggtt ttattgccgc cggtctgctg ctggggatag
    43801 cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggcacg ctacccgacg
    43861 cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac cttcctggtg attctggtgg
    43921 gctataacgc cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt attgccgcac
    43981 tctttttgct cggttataac cccgctgcaa ccaccggtta ctacgccggt tttcacgatt
    44041 tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgttgatt gccgcgtggg
    44101 cctgcgcccg catcgaaggc atggtgcgcc gctttatgcc cgacgatctc gacatgctgc
    44161 tgacctcgtt aatcacccta ctgatcaccg ctacgctggc ctatctgatc attatgccgc
    44221 tgggcggctg gttattcgaa ggtatgtcgt ggctgtttat gcacctgaac agtaatccgc
    44281 tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg
    44341 gctttattcc tgtttacctc gcgttaatgg acagccaagg attcaacagc ttatttccca
    44401 tcctttcgat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc
    44461 aaccgcacag tgcattacgc agtcaggtac gcggggcgat tattcccggc ctgctgggcg
    44521 ttggcgaacc gttgatttat ggcgtcaccc tgccccgcat gaaaccgttt attaccgcct
    44581 gtttaggcgg cgcggcgggc ggtttgttta tcggattgat agcctggtgg ggtctgccga
    44641 tgggcttaaa cagcgccttt gggccgtctg gtctggtggc gctgccgctg atgacttcag
    44701 cacaaggcat tcttccggcg atggcagtct acgctggcgg gattctggtg gcatgggttt
    44761 gcgggtttat tttcaccacg ctttttggct gccgtaacgt caatctggac tgatatcatg
    44821 aaacggacaa tgctctatct ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa
    44881 taccctgttc tgacagaaag ttcgccagag aaagcagggt tcaacgtcga acggcttaac
    44941 cagatggatc gctggattag ccagcaagtg gacgccggtt atcccggcgt aaacctgctg
    45001 attataaaag ataatcagat tgtgtatcgc aaagcctggg gagcggcgaa aaagtacgat
    45061 ggcagcgtat tgatggcgca gcctgtaaaa gctaccaccg ggacgctgta tgacctggcc
    45121 tcaaacacca aaatgtatgc cactaacttc gctctgcaaa agctaatgag cgaaggcaaa
    45181 ctgcatcctg acgatctgat tgcgaaatat atttcggggt ttgccgatag tccaaatgac
    45241 accatcaaag gcaaaaacac gctgcggatt tctgacctgc tgcatcacag cggcggtttc
    45301 ccggcggacc cacaatatcc gaataaagcg gtcgcgggcg cgttatattc ccaggataaa
    45361 ggccagacgc tggagatgat caagcgcacg ccgctagagt atcagcccgg cagcaagcat
    45421 atttacagtg atgtcgatta tatgctgctt ggatttatcg tcgagtcggt caccggtcag
    45481 ccgcttgacc gctatgttga agagtcgatt tatcgcccgc tcggcctgac acatacggta
    45541 tttaacccgc tgctgaaagg ctttaaaccg caacaaattg ccgccacgga attgaacggc
    45601 aatacccgcg atggagtgat ccacttcccg aatatccgca cctccactca ctggggtcag
    45661 gtgcacgatg aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc aggtttgttt
    45721 tccaataccg gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg gggctatggc
    45781 gatgtgcagc tgttcagtgc ggaaacggtg aagatgttca ccaccagctc taaggaagat
    45841 gccacttttg gcctcggctg gcgcgtgaat ggtaatgcca ccatgacgcc gacgtttggc
    45901 acgctggcaa gcccgcaaac ctacggtcac actggctgga ccggaacggt gaccgttatc
    45961 gatccggtga atcatatggc gattgtgatg ttaagcaaca agccacattc gccggttgcc
    46021 gatccgcaaa agaatcccaa tatgttcgaa agcggtcagc tgccgattgc aacgtatggt
    46081 tgggtagttg atcaggtgta tgcggcgtta aagcaaaagt aataaaaagg ccagtcagta
    46141 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc
    46201 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    46261 acgcttacca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    46321 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    46381 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tcaggtgaga
    46441 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    46501 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    46561 cacaaacaca tagctgccgc cggcatccac gccgtctttg ataaccgcca cttcgcgacg
    46621 cgtctcttca cccgccgggt tttctgtgcc gtcaacaaag ccgctcaggt cacgctcttc
    46681 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    46741 cgccgcctgg gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    46801 atcgaactgg gtggtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    46861 tgccccaacg ccgccgctca gagcgcgcca gatgttgtta ccaaaggcaa ccaccgcacc
    46921 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    46981 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    47041 gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataataa
    47101 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc ggcggcggtt
    47161 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    47221 tcgtccgaag gcattaaccc ttcaggacca ctccattccc cgctgaactg gtagctaata
    47281 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    47341 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    47401 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    47461 atcaccatcg ccacgttgtc gcccttcatc acttcaaaaa agcgcaccac gttgccgttc
    47521 gcggttttca ttccgctgcg caggcgataa tcgccatcaa ccgcatcggc aatggcttgt
    47581 tcttgcagtg gtgtggacgc cgttaattca ccaacgccct gctcgctcac tttggtggac
    47641 gacccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    47701 ccggtcagca tcaatggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    47761 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    47821 acttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    47881 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    47941 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag tacacgccag
    48001 gtgcgaggaa acgtagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaatgcca
    48061 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    48121 ccctgctcgc gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    48181 agcacaagcc agaagagcaa gggtaaacgc aggcgaccgt gcgcatcata atggacagga
    48241 tgaaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    48301 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    48361 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    48421 ggcaatccca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    48481 cccgtcataa ccgcccatca ccgttccgac cacttcaccg ttcacctcag cgaccagaaa
    48541 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    48601 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    48661 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    48721 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    48781 caactattga aatgaaaagt aaaacaattc tcaacagcaa acggtcgtaa cggattacgc
    48841 gatacgatat aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa
    48901 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttggc tgccctgacg
    48961 ttgtcaggaa tgtcgcaagc catcgccaaa gacgaacctt taaaaaccag caacggacac
    49021 agcaagccga aagctaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    49081 ggcgggattg ataccggagc gatcgggcgc aacggttcga aagaaaaaca tgtggtgctg
    49141 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    49201 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    49261 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    49321 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    49381 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcatctattg
    49441 cagcaagtgc tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgctcggc
    49501 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    49561 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    49621 atcaccaacc cggaagaaga gcgactgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    49681 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    49741 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    49801 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    49861 agctggcagc gcgaaggtgg cggcggcggg cgtagtcggg tgttgcgtaa tggtggtgtt
    49921 ttcgaacagg caggcgtcaa cttttctcac gtccacggcg aggcgatgcc tgcttccgcc
    49981 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttctctggta
    50041 gtgcatccgc ataatccgta tgttcccacc agccacgcga atgtgcgctt ttttattgcc
    50101 gaaaaaccgg gtgcagagcc agtctggtgg tttggcggcg gcttcgattt aacccctttc
    50161 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgcctgcca
    50221 tttggtaaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    50281 catcgcaacg aacagcgcgg tattggcggg ctgttctttg atgatctaaa cacgccagat
    50341 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    50401 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc
    50461 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac tctgtttggc
    50521 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    50581 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    50641 gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac
    50701 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    50761 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    50821 cggcgtacgg cgttcaggcg aatgcgtttc agccacgcgt tcgggccaat tcctaaaata
    50881 gcgtgaaacg cgttttgtag cgtgcggcgg ctgacatgca gttgattaca caagtccagc
    50941 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    51001 cggtaactct gatgactgat gctttccgcc gtcaccattg gctgcgcttc ttccagcatc
    51061 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    51121 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    51181 ttatgctgct ctttcacttc cagcgccgac tggctacgca acatatgtaa tacccgatcc
    51241 ggattatgca aaaagttagc ctgacgggtg atgacatctt cagaaagcac tacgcccagg
    51301 atcgtgtaat cgtccggcgt acttaattca aactcggttc caccagggcg cgtcgctatt
    51361 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    51421 ccaaaccaga acgagttcgg ccagaccaga cacgactgac gcagcgccag accggtgtat
    51481 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    51541 ggatgcagct gatcgtaaat ctgctgccag gcggtaatcg ttaaagcatg ttcatagaca
    51601 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    51661 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    51721 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    51781 taacgactac tgcgtttacg taacgtccca gcagaaaaga gctgttcgag cgcatttctg
    51841 gctttttcca gcggccagcc aaagtgggca gccacttctc ctgccgtcac tccctgacgt
    51901 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    51961 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    52021 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    52081 caacctgcgt ccagcgcagc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    52141 cgacctgggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    52201 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    52261 attggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    52321 gtaaaggcgt tacaggccgc tttacacgct gcctggctac cggttaaaaa tgctgccgag
    52381 tagttagttt cagacggtgg cgggacatag gtcaccagct gtacgtcggc agatttcaac
    52441 gctgcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    52501 ccaagcgtga tcccggcggt tgatgagaga taagaaccgg tacgcgaaac tacatgtgcc
    52561 aggaatgccg tatcttgcgc atcattagcc cactggaaag cagcgccatt ttcaatatgc
    52621 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    52681 agcacctcac cggcagtcgg tgacgggccg tgtgccgcgc ccgcatacag cgagcgacca
    52741 tataccactt ctaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    52801 tcagaatcgg cagaaatcag accgaggcta cgaatatgtg gcggcaattt cagttcacgc
    52861 gcaaaatcgg cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    52921 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    52981 agcatacgtt tcgccaaatc cacaatgacg gctgcggctt caaccggcgg cgtgccgccc
    53041 tggtgaatgt tagagataca ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag
    53101 taaacggcgt agcaggagag gctttctgac tgacccaacc ccggacgttc gcccaccagc
    53161 aggatcacta ctttcgcgcc gaggatctca ccgatctgat cttcaatctt cacgcgacca
    53221 taacgaacaa agaatggcgt gccgactttc agcccggcct gtttcaggcc cgccatcagc
    53281 ggcggcagga tctcttcgta gttcacagtg atggcatcag ttgacaggcc atcagaaata
    53341 acgacctgta cgtccggatt agcaacacac tgtgctttca gcgcttcaac agcttctgca
    53401 cacaggcggc ggcccatatc cgggcgagtc aggtagagat ttttgtcgct aatttcggag
    53461 cgtacttcca gcaagccctg cgctttcacc cactcttccg gcacttcttt cagtacggta
    53521 tctttcgaac gagagtgatc ggccaggaaa cgcagcagcg ccagcgtacg cggacgcgga
    53581 ccggcacgac cggtacaaac gcgggccacg gtgctgcgac gcagttctgt taacacatcc
    53641 gcacgatgtg ggttttcaac accaatccac acttttgctt ccgcagaacc taaatccagc
    53701 gcgcagcttt ccgaggtcac cggtgccgca caggtggtag tggcgcactt tgcttctgac
    53761 ggcgcggggg ccgtttgtcc cattgacgcc atcacgctgc gtacaatttc ttcaatctgt
    53821 ttttgatcca tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc
    53881 gtttggtcag gcgaccgttt gccataatgc ccatgctttc cagccagcgt tcaaactccg
    53941 gtgacgggcg caggttgagt aactgacgca cagtggcggt atcgtggaat gcggtggtct
    54001 ggtagttgag catgatgtca tcacccagcg gcatccccat gatgtagttg cagcctgcgg
    54061 tggcgagcag gatcatcagg ttttcgttga ggttctggtc agcgtcagcg tggttggtgt
    54121 aacagcagtc acagcccata gagatgccgc tcagcttgcc cataaagtga tcttctaagc
    54181 ccgcacggat aatctggcgg tcgttgtaga gatactccgg cccaataaag ccgaccacgg
    54241 tgttgacgat aaacggatcg taatgacgcg ccagcccata gttacgtgct tccatcgtca
    54301 cctggtctgc gccgaagtta gcgccagcgg atagcgctga accttgtccg gtttcgaagt
    54361 agaggcagtt ttccccggcg atacagttga actctgcacc cactgcgcgc gcttcgtcga
    54421 gcatcgccag ttccacgcca aactctttca gccctttttc gctgccacag atactctgga
    54481 aaatcagtcc gccaggcgcg ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca
    54541 gtacgcagcc ctgagttggg atgttgaatt tgtcgatcac gccatagatg gtgtccagca
    54601 cgcggcttaa gttttccacg tcgtcagtca ccgggttaac gccgatcacc gcatcgccca
    54661 ccccgaagga aagcccttcg tagatttgcg cggcgatact ttgcacgtcg tcacgggtgt
    54721 catttggctg caaacgggcg ctgaaggtgc ccggaatacc gatggtggta ttggcctttt
    54781 tgattaccgg cattttcttc gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta
    54841 ccgccgcgac cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg
    54901 tttcatcgct cagcacatac tcacgcagtt cgctgatgct ccagttttta atctggttgt
    54961 aggccgtttc gttaacatcg tcctgaatca gccgcgtcac gcagtcatct tcataggcaa
    55021 tcaccggatt attgcggatg tccgctacgg tcatttccga caacacctgc tttgccgcca
    55081 cgcgctcctg tgagcttgcc gctgcgacgc ccgccagcac atcccccgaa cacagttcgt
    55141 tggctttagc cagcacctct tttacatcct taaactgata tacattgccg aacaatgtgg
    55201 tctttagttt cataagtcgt tccctcagga aggaaatgcg agtgatttca ccgtcaccgg
    55261 cacaaccgat ccgccaaaaa gaggcgtacc aatgtcgata tagtcccccg cgcggacaat
    55321 cacttcgtca atgactgcca acgggagttg ttgtagctgt gggcgcaaca gcatgcccag
    55381 agctttacca aagtcctgcc cggccaccac cagcagggga tgcggattcg gaaaacgcgc
    55441 gacgaaatcg accaacgcgt tgatgaccgt cagtaccgcg gcgtaacgca caggcagcga
    55501 ggcgggaagc gccagcacgt acgcgtcagt tttgggatca agatccagct gaatcagcgc
    55561 ctgttgccag gcactcacca gatccgtttc atcaatcggg atcgccaccg gcaaattgcg
    55621 cagcggcagt tgtacgccct ccagccagat tgtgctgcca gagagcgaaa gggtatgtgc
    55681 gcccgcgcca atcaccgtgg cgcgcacggt ttgcgccgga aactgcacgt tcatctcacg
    55741 caggcgcgga tggtcatgta gcgccgtcgc cagtagcggg ccaatatcgg caaaacagaa
    55801 cgggtcagcg ggctggtggc gataacattc gcccacgccg ccagaaagcg taatgatttc
    55861 gggcgtaaca cctgcgggca gcaaaccggt ttgcattaat gcctgcgcga gcggcgaaag
    55921 cgttccgtca atcacttcga caatcagctc tgccatccgt cgggtaacct gcaccagctg
    55981 cgcgccggtc agcgaacgga cgtcagtgcc tgcaccgaaa cactcatcca caatcatctg
    56041 ccccggttta tgagcgtaaa ccacgcgccc ctggctgtcg gtttccagca ggcgaccacc
    56101 gacgttgagg caggcagtgc cgctgatttt tccggcatcg aacagggcgt agttcgcggt
    56161 gccaccgccg atgtcgatat tcagtacccg acacagccgt tgttcagaaa gggtttgcgc
    56221 cccggcaccg tgaccggcga tcacggattc gaggtgcggc ccggcgctgg cgacgacaaa
    56281 atcgcccagc gactgagaga gcgccatcac cgccgggcga gcattgcggg ttttcgcgct
    56341 ttcaccggtg atgatgatgg caccagaatc aacgctttcc ggcgcaatgc ccgcagcctg
    56401 atattgctct aaaatcagcg atttcagttc cgcttctttt aaaccaccct gtttatcgac
    56461 aggggtaaag aacaccgggc tttgccagct aatttcgcgt ttgatgaatt cgtagcgcgg
    56521 cacctgcgac accgccgcac ggttaaccag ttccaggtgg gagaaaatca cctgggtggt
    56581 ggtggtgccg atatcgatac cgacgctcag tagctggcga gtgttcacga ttgtgcctcc
    56641 gcttcggttt tagccgcggt cgcgtcgtct tttggcacca gcatcatcgc cacgccaatc
    56701 gccgttacgc cgccgatcaa cttgccgaca atcatcggga agatcatggc attcatgttg
    56761 gcagcggcga agcctaagtg gtcgcccagg gcgaaagcag cggaaacggc gaaggcgcag
    56821 ttgatgactt tgccgcgggt atccatctgc ttcatcatgc cgaacatcgg gatgttgttg
    56881 gcaagcgttg ccaccatgcc ggctgccgcg atgttgttca tattcagcac tttaccgacg
    56941 ctcatcagcg gtttttcaaa ccagcgggtc agcagcagca ccatcggata cgcccctaac
    57001 agaacgcagg agatagaacc gataacttca atggcgcgca tcacctcacc gggtttatcg
    57061 ccaggggcca taaagatagg atccagaccg gggatcagtt cccagccaag caggaatttc
    57121 actaccgcag cggcaagacc gagggtgatc aatgcaacga ggaatttggc gaagatctgg
    57181 aagccgttga tcattttttc tgggatgaat ttcagcccca gcgccaccag aatcgcaaca
    57241 atgatcaccg ggatcatgtt catcaggatc agggcgaaag tgaattccac cggctggccg
    57301 ttgatctgaa caccggagta catagcaacc agaccaccag cgatacaacc aatcggaatg
    57361 gtcacaatgc ccgccagcac gccgagcgcc agataacgac ggtcagaagg ttcgataatg
    57421 ccgagcgcca ccggaatgga aaacacaatc gttggcccca tcatcgaccc gagaattaac
    57481 ccagagtata gccacgcggc tacgtcgccg cccgccagct ctttggcgag gaagaagccg
    57541 cccatatcgc acgccagcag tgttccggcg aacatcgatg ggttagcgcc gagcatttcg
    57601 taaaccggaa taattaccgg cccgagtacg tgagccagta ccggtgccag cgcggtcata
    57661 ccgaccatcg ccaggcccag tgcgcccatt gccataaagc cttcttcgaa ctgaccgccg
    57721 gatccttcga tacttttacc gaacttaccg aggaaacgag cagaaccgcc gaactgtgac
    57781 aggatcctgt caacggcagc tatcagcata aagaacatca tgatgtacat gatgatttcg
    57841 ttaattccca tcgcctttac tccctgttag ttgttattta ttagcggatg ctgcgtgaag
    57901 ccttatccgc actacatgtg caatcccaca gaccggataa gacgcggcat ttattgcgcc
    57961 gctgcgtaca ggccgacaag ctgttccagg ctggcggtac gcgggttgct gcgcagacaa
    58021 atatcttcca gcgcggcctg cgcccatgca ccgtaatgcg cagatgtcgc gccaacatcg
    58081 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    58141 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcgcgacaa
    58201 accatccggt taaactccat caccgttggc agcaacatag cgttcgcgag accgtgcgga
    58261 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    58321 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    58381 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    58441 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    58501 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa tgcggcgtcg
    58561 aggatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    58621 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt agttggaatg
    58681 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    58741 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    58801 aatgcgatca ccccgtcaca acctgactca cgcaactgcg ccacggctgc acacacgtcg
    58861 gtaatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatacc tttaacggcc
    58921 aggctacgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    58981 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    59041 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    59101 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    59161 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    59221 taacgccggg aattgtttgc gaaacagttc tgccacgccc ggttgcatac aggagccgcc
    59281 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    59341 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    59401 ctccgcctct tccagcgaaa tacggcgatt tccggcgaaa gtcagagaga tgtgatgtcc
    59461 gccggtggct tcatccgccg agtacgtcac tttgcccttt ttcacgatgg caatgccggt
    59521 agtgccaccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcgactgc
    59581 ggttggctca tccagcacat ggctcacttc cagcccggca gactccagca cgttgatgga
    59641 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac gacgaccgaa
    59701 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg accgcgccga agaaatccca
    59761 gacgatgccg tcgcgaacca catcggccca gtcgaggcat accgccactg gctgaccgtc
    59821 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccactccca gccacagcgg
    59881 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    59941 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    60001 atacacagcg acgcagacgg acaaacgtac gcgcgctggt caccccttca ccggttggcg
    60061 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcacggtc
    60121 cgttcttaac gaaaatgctg gtatcaacag cattcgccat ctggttcatg ttttcgatgt
    60181 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gctagcgcaa
    60241 tggcatccgc cacgttggcg acgcgcacga cgggcaacac cggcatcatc agttcagtca
    60301 cggcaaacgg atgttctgcg gtggtttcca caaacagcag gcgcgtttct tgcggaactt
    60361 taaggccgat tgccgccgcg attttgcctg cgtcgcgacc aacccagtca cggctgacgg
    60421 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    60481 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    60541 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagacgcgc
    60601 ctttgacgat ggactgagca gcacgggcga ggtcagcggt ttcatccacc actaccggcg
    60661 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    60721 ccgcttcgcc gccggtcacc accagcaggc cgatacccgg aaacttgaac aagcgttgcg
    60781 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    60841 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    60901 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    60961 ccgccgggtt agtggacggc gtcaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    61021 aagtcaggcc gttgtcgccc gtcagcactt gcggagagag acactcaacg cctggcgtgc
    61081 cgcgtgcctg agcgacgttt ttggcaaatt tatcttcaac gcgacccatg ccggtttcac
    61141 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcttcacga atggcagcaa
    61201 tggctaactg gcgcattgcc acgctcttta acccttgctg ggcaattttg gctgccgcaa
    61261 cagcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    61321 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    61381 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacgatgcc
    61441 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    61501 actcaccagc aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcaca
    61561 ttgcccgtca ggattaccat gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    61621 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    61681 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcggaag gtaaaatgaa
    61741 aaatgagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    61801 cgcctttcag gccgatcggg aacacttctt ccaggtcgcc gtgcggacgc gggataacgt
    61861 gtacagatac cagctcgccg atacgctgtg ccgctgcggc accggcatcg gtcgccgctt
    61921 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    61981 caccaaccag cttgacgcgc gcagctttca ccatcgcgtc agaagcctca atcagtgcaa
    62041 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    62101 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    62161 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacac tacaaccacg
    62221 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    62281 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    62341 cacattggcc ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    62401 gaaggcggcg tcaaactgca actcgccatc gactaccagc tttggtgcgc gctcacggac
    62461 gatttctgtc gcctgctgga cattggcaac gcaggggtga cgggcgctac cgttactgga
    62521 aaacgacagc atcgccacgc gcggctcttc tccggtgatg gcgcgccagg tttcggcgct
    62581 ggcaagcgcg atatccgcca gctgcgccgc cgtcggctgt ggtaccacgc tgcaatcggc
    62641 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    62701 acagcctggc tgcaagccga taatgcgtaa tccggcacgc agcacattcg ccgtggaaga
    62761 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgcgg cggcgaacat
    62821 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    62881 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcta tcacctgtag
    62941 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattggcgac
    63001 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    63061 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacaacaacg
    63121 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggctgc
    63181 ttcgttacgc tcaggatcat catcacgtag accgtgctgg aaaggcggtt cagcgcctgc
    63241 aagatgtccg ggcgtaatac ttcaaaactg cgggtgataa acacctgcgc cgctacggtt
    63301 tcggcttccc gcactttggt gcgtagcaga ttcagcaacg ccgcatcgcg cccgtggctt
    63361 gcttccggca ccagatgatc gtgaccaaga taacgcaacg gctgatgaga aagccggtgc
    63421 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttccgc cagttcaatt
    63481 tgcagccaca ctgccagcgc aatggtgctg tcgagaactg cgcgaaaacc cagacgcgga
    63541 tcgcttttgg cgaccatttt ttccgccgac aggtgagtca gcgtatccgg cttttttgcc
    63601 accggctggc ggcacagttc gcagcacgcc tgcggatgtt catcgctact ggttagccca
    63661 tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg ctcgtcaata
    63721 aacttgatgc gcagatggcg gctttccagc aactcccggg cagagggcgt caggcgactg
    63781 tccgcaggga gatggatctc tgcgccttcg ctgagcgtat ggttcgctct tagccatgct
    63841 tcggtgatga aatctttcat agggattgcc agttagccgg ccaggcgaca tacaggaatt
    63901 tcacgctgga tgtcgtacca aattcgatgc tggagccttt cgggataaac atcacgtcgc
    63961 ctgctttagc gatcatggtt tcgccttcgt ggcgaacatg gagctcccct tccagcacca
    64021 tgtcgatttc gtcgtagttc agggtccacg ggaagaatgc gttctcccac tgcataaaac
    64081 cggcggccat gctgctgccg tcgtctccgg tcacaagatc agtcaagccc acgcaatgcg
    64141 gttgtgcgcc atcaaagcga ccaaacttga cgctgctacc atcaatcact ttgatgccgc
    64201 ctttgccggt caccgatttg aagctcggct gcattgcacc ctgctccagc gactgttttt
    64261 ccttcatcac tttttccatc agctgcgcga ccaggctttc ggtaaactgg ccttccggta
    64321 gctgggcgat aatggtttcg cggatgcgct ggctttcggt tttatcggct ggcacgctgg
    64381 cgggcacgga cgccgttacc gggatcaatt catcacattc ggtgatggta aaacccagta
    64441 gatccgccac ttcacgggct tccggggtaa taatgctggc gcgcagaacc acggacattg
    64501 cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt tttttcacct
    64561 gcttcctcct gtttggtgag agatgccaga taatccacca gttgctgcac gctttgcggg
    64621 tcgtggctgt ttagctcgaa aatcggctcc tcgaagcccg tctcaagcaa taactttcgc
    64681 gttgcggcga cgtcagcatc cggcatgtcc gttttactga tgacggcgat ttgccgtttg
    64741 ctgacgccaa tatccagcaa cccggcaggc aaccgacttt ccgggtcatt cgcgccgtgg
    64801 acatagatca gcatgtccac atcctgtagc gtggtaatta aggcgtgata ccagcgggga
    64861 tggctaaaat attcacccgg cgtatcaata tcgcccttat cattaaattc cacggcctgt
    64921 gtttttctgg cgagggtata attcccctgc aacgcattaa ataacgttgt tttccctgcc
    64981 ccgacggtac ccacaaacgc aattcgtttc atgaatgcct cgtattaact tttggtcatt
    65041 tcacaaagcg tataatttaa taagcgacct aaaccactga cggtttgtga taacgcttct
    65101 tctactgcgc ccaccgagcc ataaatcacc aatgcaccac tgaagcggtc gagaaatccg
    65161 atatgcacat cggcggcttt cagcgctaaa tcaccggcga tcatcgccgt ttcgccggga
    65221 gttagcgtca taatgccaat tgcgcccgca tccggaacgc cgattttctt cgccagttcc
    65281 tcgccgggat gcgcgatgag atgcgccagt gtgacctgtt tgccgggcac aaattcctga
    65341 atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa ctttc
//
LOCUS       DBFIFS010000006        62970 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.006, whole genome shotgun sequence.
ACCESSION   DBFIFS010000006 DBFIFS010000000
VERSION     DBFIFS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.006"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..182
                     /locus_tag="N7A07_000353"
     CDS             <1..182
                     /locus_tag="N7A07_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2475095.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVK"
     gene            270..512
                     /locus_tag="N7A07_000354"
     CDS             270..512
                     /locus_tag="N7A07_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2475096.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            516..650
                     /locus_tag="N7A07_000355"
     CDS             516..650
                     /locus_tag="N7A07_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475097.1"
                     /translation="MHTAFEFWVRKTFGNRYDLNRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            669..923
                     /gene="xisR"
                     /locus_tag="N7A07_000356"
     CDS             669..923
                     /gene="xisR"
                     /locus_tag="N7A07_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU2475098.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            957..2243
                     /locus_tag="N7A07_000357"
     CDS             957..2243
                     /locus_tag="N7A07_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2475099.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2264..2965
                     /gene="mlrA"
                     /locus_tag="N7A07_000358"
     CDS             2264..2965
                     /gene="mlrA"
                     /locus_tag="N7A07_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU2475100.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3025..3132
                     /gene="yohO"
                     /locus_tag="N7A07_000359"
     CDS             3025..3132
                     /gene="yohO"
                     /locus_tag="N7A07_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU2475101.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="N7A07_000360"
     CDS             complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="N7A07_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU2475102.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="N7A07_000361"
     CDS             complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="N7A07_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU2475103.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="N7A07_000362"
     CDS             complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="N7A07_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU2475104.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="N7A07_000363"
     CDS             complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="N7A07_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU2475105.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="N7A07_000364"
     CDS             complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="N7A07_000364"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU2475106.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9594..11309
                     /gene="dld"
                     /locus_tag="N7A07_000365"
     CDS             9594..11309
                     /gene="dld"
                     /locus_tag="N7A07_000365"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU2475107.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="N7A07_000366"
     CDS             complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="N7A07_000366"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU2475108.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="N7A07_000367"
     CDS             complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="N7A07_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU2475109.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGDMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13210..13788
                     /gene="yohD"
                     /locus_tag="N7A07_000368"
     CDS             13210..13788
                     /gene="yohD"
                     /locus_tag="N7A07_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2475110.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="N7A07_000369"
     CDS             complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="N7A07_000369"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2475111.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="N7A07_000370"
     CDS             complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="N7A07_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU2475112.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            16392..16475
                     /gene="yohP"
                     /locus_tag="N7A07_000371"
     CDS             16392..16475
                     /gene="yohP"
                     /locus_tag="N7A07_000371"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU2475113.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(16863..17813)
                     /gene="dusC"
                     /locus_tag="N7A07_000372"
     CDS             complement(16863..17813)
                     /gene="dusC"
                     /locus_tag="N7A07_000372"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU2475114.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(17972..19165)
                     /locus_tag="N7A07_000373"
     CDS             complement(17972..19165)
                     /locus_tag="N7A07_000373"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HGU2475115.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(19180..19824)
                     /gene="maiA"
                     /locus_tag="N7A07_000374"
     CDS             complement(19180..19824)
                     /gene="maiA"
                     /locus_tag="N7A07_000374"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HGU2475116.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(19833..20534)
                     /locus_tag="N7A07_000375"
     CDS             complement(19833..20534)
                     /locus_tag="N7A07_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU2475117.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(20549..21577)
                     /gene="gtdA"
                     /locus_tag="N7A07_000376"
     CDS             complement(20549..21577)
                     /gene="gtdA"
                     /locus_tag="N7A07_000376"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HGU2475118.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(21589..22947)
                     /locus_tag="N7A07_000377"
     CDS             complement(21589..22947)
                     /locus_tag="N7A07_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2475119.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            23074..23982
                     /locus_tag="N7A07_000378"
     CDS             23074..23982
                     /locus_tag="N7A07_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2475120.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            24113..24511
                     /gene="yohJ"
                     /locus_tag="N7A07_000379"
     CDS             24113..24511
                     /gene="yohJ"
                     /locus_tag="N7A07_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU2475121.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            24508..25203
                     /gene="yohK"
                     /locus_tag="N7A07_000380"
     CDS             24508..25203
                     /gene="yohK"
                     /locus_tag="N7A07_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU2475122.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            25333..26217
                     /gene="cdd"
                     /locus_tag="N7A07_000381"
     CDS             25333..26217
                     /gene="cdd"
                     /locus_tag="N7A07_000381"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU2475123.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            26367..27086
                     /gene="sanA"
                     /locus_tag="N7A07_000382"
     CDS             26367..27086
                     /gene="sanA"
                     /locus_tag="N7A07_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU2475124.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            27089..27328
                     /gene="yeiS"
                     /locus_tag="N7A07_000383"
     CDS             27089..27328
                     /gene="yeiS"
                     /locus_tag="N7A07_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU2475125.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            27522..28760
                     /gene="preT"
                     /locus_tag="N7A07_000384"
     CDS             27522..28760
                     /gene="preT"
                     /locus_tag="N7A07_000384"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU2475126.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            28754..29989
                     /gene="preA"
                     /locus_tag="N7A07_000385"
     CDS             28754..29989
                     /gene="preA"
                     /locus_tag="N7A07_000385"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU2475127.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(30232..31242)
                     /gene="mglC"
                     /locus_tag="N7A07_000386"
     CDS             complement(30232..31242)
                     /gene="mglC"
                     /locus_tag="N7A07_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU2475128.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(31258..32778)
                     /gene="mglA"
                     /locus_tag="N7A07_000387"
     CDS             complement(31258..32778)
                     /gene="mglA"
                     /locus_tag="N7A07_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU2475129.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKRDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(32839..33837)
                     /gene="mglB"
                     /locus_tag="N7A07_000388"
     CDS             complement(32839..33837)
                     /gene="mglB"
                     /locus_tag="N7A07_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU2475130.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(34116..35156)
                     /gene="galS"
                     /locus_tag="N7A07_000389"
     CDS             complement(34116..35156)
                     /gene="galS"
                     /locus_tag="N7A07_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU2475131.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(35298..36455)
                     /gene="yeiB"
                     /locus_tag="N7A07_000390"
     CDS             complement(35298..36455)
                     /gene="yeiB"
                     /locus_tag="N7A07_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU2475132.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(36472..37140)
                     /gene="folE"
                     /locus_tag="N7A07_000391"
     CDS             complement(36472..37140)
                     /gene="folE"
                     /locus_tag="N7A07_000391"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU2475133.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            37398..38234
                     /gene="yeiG"
                     /locus_tag="N7A07_000392"
     CDS             37398..38234
                     /gene="yeiG"
                     /locus_tag="N7A07_000392"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU2475134.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(38266..40245)
                     /gene="cirA"
                     /locus_tag="N7A07_000393"
     CDS             complement(38266..40245)
                     /gene="cirA"
                     /locus_tag="N7A07_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU2475135.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(40537..42006)
                     /gene="lysP"
                     /locus_tag="N7A07_000394"
     CDS             complement(40537..42006)
                     /gene="lysP"
                     /locus_tag="N7A07_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU2475136.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(42211..43092)
                     /gene="yieE"
                     /locus_tag="N7A07_000395"
     CDS             complement(42211..43092)
                     /gene="yieE"
                     /locus_tag="N7A07_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU2475137.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            43191..44240
                     /gene="yeiH"
                     /locus_tag="N7A07_000396"
     CDS             43191..44240
                     /gene="yeiH"
                     /locus_tag="N7A07_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU2475138.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            44314..45171
                     /gene="nfo"
                     /locus_tag="N7A07_000397"
     CDS             44314..45171
                     /gene="nfo"
                     /locus_tag="N7A07_000397"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU2475139.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            45174..46262
                     /gene="yeiI"
                     /locus_tag="N7A07_000398"
     CDS             45174..46262
                     /gene="yeiI"
                     /locus_tag="N7A07_000398"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU2475140.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(46318..47568)
                     /gene="nupX"
                     /locus_tag="N7A07_000399"
     CDS             complement(46318..47568)
                     /gene="nupX"
                     /locus_tag="N7A07_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU2475141.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(47668..48609)
                     /gene="rihB"
                     /locus_tag="N7A07_000400"
     CDS             complement(47668..48609)
                     /gene="rihB"
                     /locus_tag="N7A07_000400"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU2475142.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            48739..49437
                     /gene="yeiL"
                     /locus_tag="N7A07_000401"
     CDS             48739..49437
                     /gene="yeiL"
                     /locus_tag="N7A07_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU2475143.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(49508..50758)
                     /locus_tag="N7A07_000402"
     CDS             complement(49508..50758)
                     /locus_tag="N7A07_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU2475144.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(50852..51790)
                     /gene="psuG"
                     /locus_tag="N7A07_000403"
     CDS             complement(50852..51790)
                     /gene="psuG"
                     /locus_tag="N7A07_000403"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU2475145.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(51778..52719)
                     /gene="psuK"
                     /locus_tag="N7A07_000404"
     CDS             complement(51778..52719)
                     /gene="psuK"
                     /locus_tag="N7A07_000404"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU2475146.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(53143..54834)
                     /gene="fruA"
                     /locus_tag="N7A07_000405"
     CDS             complement(53143..54834)
                     /gene="fruA"
                     /locus_tag="N7A07_000405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU2475147.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(54851..55789)
                     /gene="fruK"
                     /locus_tag="N7A07_000406"
     CDS             complement(54851..55789)
                     /gene="fruK"
                     /locus_tag="N7A07_000406"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU2475148.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(55789..56919)
                     /gene="fruB"
                     /locus_tag="N7A07_000407"
     CDS             complement(55789..56919)
                     /gene="fruB"
                     /locus_tag="N7A07_000407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU2475149.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            57287..58468
                     /gene="setB"
                     /locus_tag="N7A07_000408"
     CDS             57287..58468
                     /gene="setB"
                     /locus_tag="N7A07_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU2475150.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(58465..58719)
                     /gene="yeiW"
                     /locus_tag="N7A07_000409"
     CDS             complement(58465..58719)
                     /gene="yeiW"
                     /locus_tag="N7A07_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU2475151.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            58874..59446
                     /gene="yeiP"
                     /locus_tag="N7A07_000410"
     CDS             58874..59446
                     /gene="yeiP"
                     /locus_tag="N7A07_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU2475152.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            59669..61135
                     /gene="yeiQ"
                     /locus_tag="N7A07_000411"
     CDS             59669..61135
                     /gene="yeiQ"
                     /locus_tag="N7A07_000411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU2475153.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            61253..62239
                     /gene="yeiR"
                     /locus_tag="N7A07_000412"
     CDS             61253..62239
                     /gene="yeiR"
                     /locus_tag="N7A07_000412"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU2475154.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            62278..>62970
                     /gene="lpxT"
                     /locus_tag="N7A07_000413"
     CDS             62278..>62970
                     /gene="lpxT"
                     /locus_tag="N7A07_000413"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU2475155.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKN"
BASE COUNT        15404 a        16819 c        15685 g        15062 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggga agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaaat
      181 gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg catttattct
      241 gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt ggtcagatat
      301 acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac tcagtgttct
      361 ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac aacctgttcg
      421 aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag gaaatgcgcc
      481 tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt gagttctggg
      541 ttcgcaagac attcggcaat cgctacgacc tgaaccgtga tgtcgacggc ttctactgcc
      601 gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga aagttttatg
      661 aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt acggagagag
      721 tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga agaaagtcat
      781 ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg cattacagtg
      841 aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa caaccaggtg
      901 ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga ggtttaatga
      961 gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgt atatggtttc
     1021 actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagcc aaaaaccgga
     1081 agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg agtttcgact
     1141 acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga aaaagagaga
     1201 taaccgttaa ggcactttcg gaaaaatggt tggaccttaa gaaaattgag atttgtgcga
     1261 atgcacttaa ccgttaccag tcagtaatta aaaacatgtt accaatgtta ggtgaaaaaa
     1321 aactggtttc atccataaca aaagaggatt tacttttcgt aaggagagat ttgttgaccg
     1381 gttaccaaaa gctttctaat ggaaagactt cttccataaa agggcgctca gtggtcacgg
     1441 taaactacta tatgacaacc atagctggaa tgtttcaatt tgcaacagat aatggttata
     1501 cctcaggaaa cccatttaac ggtctggctc ccttaaaaaa gtccaaggta aaaccagatc
     1561 ctctcacccg tgacgaattt attcgtttta ttgaggcttg ccgtcatcaa caaacaaaaa
     1621 acctgtggat tctcgctgta tacacgggta ttcgtcacgg ggagttggta tcgctggcat
     1681 gggaagatat agaccttaaa gcaaggacta taaccatccg tagaaattat acaaaacttg
     1741 gcgaattcac tccaccaaaa accgatgcag gcaccggaag gacaattcat ctggttcaac
     1801 cagctattga tgctcttaaa agccaggcgg aaatgaccat gcttggaaag caacattctg
     1861 tagaggtgaa gcagagggaa tatgggagaa ctgctgtgca taaatgcact tttgttttta
     1921 gtcctcaggt aacaaaacag cagcagttgt ccggacctca ctacaaggtt gactccatca
     1981 gggagtcatg gacaagtatc ttaaaacgcg caggtctgag acacagaaaa tcgtaccaat
     2041 ccaggcatac ttatgcatgc tggtcacttg ccgcaggagc taatcctagt tttatcgcaa
     2101 gccagatggg ccacacaaac gcacaaatgg tattcaatgt ttacggagca tggatgaaag
     2161 acaacaatca cgaacagata gaactcctta acaaaagact atctgaaagt gtcccatgta
     2221 tgccccataa gaaagcaggg taaaataaaa acttgcaaaa tcaattagtt tacccttaat
     2281 ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg ctgaaaccgc aacggacaga
     2341 cggcggtcat cggctgttca acgatgccga tattgaccgg atccgcgaga tcaaacgctg
     2401 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     2461 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     2521 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacgct
     2581 caccacacat ctgtttattc ctctgcgccg acggcttcag taccaacaac cgactctcca
     2641 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     2701 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     2761 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     2821 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     2881 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     2941 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3001 tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat cgccctgttc
     3061 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     3121 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     3181 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     3241 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt
     3301 tgataatcac cgaggttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     3361 ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct gtcacgctgg
     3421 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccataaagga
     3481 tcaaggcgat aatcgctggt tgcagaccaa agcctatcac cggaacagca atcgccagca
     3541 ctgcgacagg cggaaaagtc tgcccaacgg cggcaatagt ttccaccagt gggcgaaatt
     3601 ccgcgcccca cgggcgagtg actgcaattc cggcaccagt gccaatgatc accgcaaaca
     3661 aactcgaaat tcccaccagc cagaaatgag ccagtgccaa agctgcaaaa ctttcttgct
     3721 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     3781 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     3841 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgct aggctggccc
     3901 tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     3961 cgcagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4021 tccgccacac tacgtaacga aagcaggcgc acacccagtt cactacgtcc aaaaaactgg
     4081 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     4141 tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc aatatcatgc
     4201 gtgaccagca caatggtacg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     4261 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     4321 tcggcagcca gtgcgcgcgc tactcccaca cgttgctgct gaccaccgga aagctgatgt
     4381 ggataacgct cacgtaaatt tgactccagc cccagtagcg ccattaattc gtcgatacga
     4441 tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca cggtggcaat gttttgtgcc
     4501 acgctccagt ggggaaacag gccaatagat tgaatggcat agcccatacg gcggcgcaac
     4561 tccagtactg gcagcgagcg aatttcttct ccagcaaagc ggagctctcc gctgtcatgt
     4621 tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga tgtgccaatc
     4681 agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc tttttgtgcg
     4741 ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca acagtgcgat
     4801 tagtaaatcg aacagcgcat cgatcagcac cgccagaaca attaccggga tcacccccag
     4861 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     4921 tgcgccgatt aacgccgcaa tcaccgccat gcctacagtt tgcaccatca ccacccgcag
     4981 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5041 gctcatgccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagccgac
     5101 taccacgccg cgcaccagcg gtagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     5161 cattccggtt cctgctatgc caagcttccc cagccacgga aaggccgtaa ccagtccggc
     5221 aagcggcgca atcagcaggc caaagagcgc cacggaaggc acggtctgaa tgacattgag
     5281 cagagaaaaa atcgccccct gccgcgcggt ggaaaagtag caccagatgc ccaacggcac
     5341 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     5401 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     5461 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     5521 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccga
     5581 agccaaccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     5641 ttgggtcgcc gcctttccag ctccccacac cagcaatacg aagacgaatt gcgccagaat
     5701 gagtgcaaaa atgctgcctt ttttagcggg aataaaacag gctgtcagcc aggcgcaacc
     5761 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     5821 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     5881 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgt
     5941 ccaccctttt tgtttcaagt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6001 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6061 ccactgtgcc atttgcggat actctttcag caccgactca cgcaccactg gtgcaggcgc
     6121 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agcccctgcg ccgcgaccgg
     6181 accgtcagtg ccgtaagcca ttgcggcatt aacgccagaa gtttgctggg cagcggcttt
     6241 aatcgttacc gccgtgtcgc cgccagccag tgacagtaac tgatcctgat cgagtttaaa
     6301 gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact cagccgaggc
     6361 tgccagtttg aaggtgccgc cctctttcag ataacgcctc aggtcagcaa gcgaagtgag
     6421 tttgtttttc tctgccacat cctgacgcac ggcgatggtc caggtgttat ttgcaggtgc
     6481 gggcgtcagc cagattaact tgttttgctc tgcatcgagt tttttgactt tctcgtaacc
     6541 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaaaaacg cgccattgcc
     6601 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     6661 cgttccaagt tgcactttat tgaccgttgg tactccgtgg ctttcgagta cttgcaaaat
     6721 gatattgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     6781 cgcgtgcagc ggcaggctca cggctgccaa caaaaccagt gaacctgccc agacctttga
     6841 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     6901 atagttgata atggcgggat tagcctgagc ggacggattc agttgcagaa tcagataaat
     6961 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttaaccg
     7021 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     7081 gtcgcgccct tgctaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     7141 tcagtgccga taaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     7201 cagaacttca gcgcctcgat atcaatcggg aagctgacgg tctgggtttc gcccggcttc
     7261 agggtgattt tctcaaagcc tttcagctgc ttcactggac ggctcatgga agccgtcaca
     7321 tcctgcaagt acatctgcac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
     7381 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     7441 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     7501 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     7561 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagctta
     7621 ccggacgggt tgtaatcgcc aaacaacaca tcggcaattg cattaccgcc ttcagtcccg
     7681 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     7741 cgcccgttca tcagcaccag caccagcggt ttgccggtgg ctttcagggc ggcaatcaaa
     7801 tcacgctggc tttgcagaat agtaatatcg gtacggctgg aggcctcgtg cgccatcccc
     7861 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     7921 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     7981 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     8041 ttttcaccga cggcattttt aatcccggtc agcacggtca cggattgatc ggcaacacct
     8101 gccgccgacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     8161 atggtggctg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
     8221 tcacgcgcca cttcacgcgc ttctttgcgg tgcaggcggc tttcggcatt ggtatccacc
     8281 gggtcagact ctttcggacc gagatggctg tatgggtcgt taaacaaccc catatcatat
     8341 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatcgtcac tttaccggat
     8401 ttgatcaacc caggcagata cttcgagtag tactcgtcgc tcatgctcat gttgattccg
     8461 gatttcagcg ccacgcgcac tgcatcttcc ggatctgccg ccgtgccatg tttaatcagc
     8521 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     8581 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     8641 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     8701 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     8761 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     8821 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     8881 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     8941 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt ttttactgca
     9001 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     9061 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     9121 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tagccccaac ctgaccgtct
     9181 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
     9241 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
     9301 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcagggcc
     9361 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     9421 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     9481 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     9541 acaaggcgct attctagttt gtgatgtttt ttcgccacca caaggagtgg aaaatgtctt
     9601 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     9661 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     9721 atggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     9781 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     9841 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctac
     9901 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     9961 cgctttattc cctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    10021 gttcatcgtg cattggcgca tcggttatcg gcggtatctg taataactct ggtggttcgc
    10081 tggtgcagcg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagatg
    10141 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    10201 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    10261 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    10321 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    10381 ttgcagtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    10441 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    10501 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    10561 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    10621 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    10681 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    10741 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    10801 aagccaaatc gtggctggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    10861 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    10921 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    10981 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    11041 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgttaaaa
    11101 aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga gctgctacag cagcgcggcg
    11161 cgcaataccc tgccgaacat aacgtcggtc atttgtataa agcaccggag acattgcaga
    11221 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggatcggt aaaaccagta
    11281 agcggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    11341 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    11401 tttatagctt aacgctgctg ccggaacagg catcacttta ccggtttcaa tccaggtacg
    11461 caggcggctg gcatcggcaa aatgggtgta tttgccaaac gcatccatca ctaccagcgc
    11521 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    11581 gccggttttg gttagctgga tattccagtt atcgcgatac accagatgat tagtattgcg
    11641 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    11701 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcggt
    11761 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    11821 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    11881 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    11941 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    12001 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca cgaccatcgc
    12061 agtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    12121 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    12181 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    12241 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    12301 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    12361 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    12421 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    12481 agtataacgg taagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    12541 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    12601 agtaggtaac cggtgtaaaa cagtgccacc gtgccgacca gcgcacacaa ccagaccagc
    12661 ggataaagcg ccaccagacc acttaaaaac agcggagttg caacgtagcc cgcgaagacc
    12721 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    12781 cccatcaccg cgaccccagc gagcatatcg ccataaaaca ggacagccag cgccagtccg
    12841 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    12901 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    12961 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    13021 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    13081 gtataagtca gcttgtgatt attttttgtt cgcccatgta atttgcaccg tctgatgtac
    13141 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    13201 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    13261 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gccttgcggc acatcagggg
    13321 ctattaaagt tcccgctggt ggtactttct gtagcgcttg gcggcatgat tggcgaccag
    13381 gtgctctatc tgtgcgggcg gcggtttggc ggtaagctgt tacgccgttt ctctaaacat
    13441 caggataaaa ttgagcgggc gcagaaactg atccaacgcc atccgtatct gtttgtcatt
    13501 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    13561 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    13621 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    13681 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    13741 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    13801 ggataaggcg tgcgcgccgc atccgacatc cggttcacca tgcctgatgc ggcgcttgtg
    13861 cgtcttatca tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta
    13921 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    13981 ggtggtgtaa tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatgcgt
    14041 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc
    14101 catgccgttc attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg
    14161 cctgaccaac tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    14221 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    14281 accaccctgt ccttgtttca ccatctggcg ggcagcaatt tgcgagcata aaaatgcacc
    14341 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcac
    14401 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaataag
    14461 tttctccagc gcctgcgcac cttctggcag attgccgaga tccagttgca cgatctccgc
    14521 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    14581 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    14641 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    14701 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    14761 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    14821 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    14881 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    14941 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    15001 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    15061 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    15121 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    15181 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    15241 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    15301 gttcatcagg aagctggctt gccactttcg gcaacgccac cggtttaagc gtcaatgact
    15361 tagtctggtg atttgtgagt gcaattaacc gtgcctcaat aattttcatt ttccccgcga
    15421 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    15481 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    15541 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    15601 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    15661 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    15721 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    15781 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    15841 cagaaaagtc gatctgcgga ccatcctgag tagcaactgc cttcgcctgg gcttcagcta
    15901 actgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    15961 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    16021 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    16081 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    16141 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    16201 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    16261 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    16321 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    16381 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    16441 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatgaca tataagttgt
    16501 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    16561 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    16621 cgcgtcttac aaggacgttt cctcgcgccc gacccggtag ccgaagggga taagctgaag
    16681 gtaccgcgca gcggcgattt tgttcgccag agcccggggg tgcaggggga ggaggcgatt
    16741 gtcctccccc tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag
    16801 cgcaacaaga tgatgaagct gaataagcga ttcattgttt ctgataaact gcgattccac
    16861 tattatcgca gcttaccgat atcaattgcc tgaatagccc ttgcaatatc aggggaatta
    16921 ttcaacacac gaacatgctg aaataattct gttgcttcat cgtattcttt acgcaaataa
    16981 ctcaaccact gtttaatccg cgctacgtga tataacccgg tatcgccctg cttttccaga
    17041 cgggtatatt tttgcagcaa agcaaccacc tccggccacg gcattcgcgg ttcgttatat
    17101 tttactaccc ggctcaggtt gggaatattg agcgccccgc gaccaatcat cactgagtcg
    17161 cagccgctga tcgccatgca ttcttgcgcg ctctgccagt cccagatttc gccgttggca
    17221 atcaccggaa tattcagccg ctggcgaatc tcgccaatcg cctgccagtc aatatgctcc
    17281 gcgcggtaac cctgctcttt tgtccgtcca tgcaccacca gctccgtagc accagcctgt
    17341 tgaaccgcat cggcgatttc aaatttcttc tcaccgctgt cccagcccag acgcactttc
    17401 acgctgacgg gcaaatgcgc cggtacagct tcacgcatcg cttttgcacc ctggtagatg
    17461 agttcaggat ctttgagtaa cgtcgcccca ccaccgctac cgttaaccgt tttcgacggg
    17521 cagccgcaat tgagatccac gccccaggaa cctaattcca ccgcacgggc ggcgttctct
    17581 gccagccatt gtgggaactg acccaacaac tgcacgcgca ccagcgtacc ggatggtgtc
    17641 cggctggcgt tttgtagctc agggcaaatg cgatgaaaga ctttcaccgg cagcagttga
    17701 tccaccacgc ggacgaactc ggtgatgcac agatcgtagt cgttaacttc ggtcagcaat
    17761 tcacgcacca gagagtcaag cactccctcc atcggagcca gtaacacacg catatcatca
    17821 cccgcaaaaa atgaggcgct atgttagcgc ctcctgtgtt tgcagacaaa ctgacttact
    17881 gattaattcg ggcaagattt ttaccctcac cctaaccctc tcccgaaggg gcgaggggac
    17941 cgcccgtgcg cctgattggg tgaagaagtg cttagcgtgg ttgcaaacag ttatcttctt
    18001 tccagccgta caaccactcc atgccgcgat aaaattctgc ctgcgatttc cctttccaca
    18061 gcagattacg tacctggcgt tctacccctg cggcgtggta aacccgcccc atttcacggg
    18121 tcgaccagac gatgcgcgcg gtgcgcggaa ttcgtaccga ctcatacagc gcaaaggctt
    18181 gcgccgcatc accctcacac tgcgccagcg ccttacccag cgttactgca tcttccagcg
    18241 ccatacaggc tccctgcgcc atatattgcg ccaccggatg ggccgaatcg cccacaagcg
    18301 ttatgcgctt cgttccccac ttcgccaccg gttcacggtc agcggttgac cagcgtcgcc
    18361 atgaagtcgg tttatccaac atctggcgcg gacgggggtg aatcccttca aagtaagaga
    18421 gcacttcctc tttactacca tccttcacgc cccactcttc ctgctggcgg ctgtggaatg
    18481 ttaccaccag gttatactgc ttgccgccgc gcaaagggta atgtaccaag tgacaatgcg
    18541 gccccgccca cagtaccggt gcgttaatgc gcaaatcttc cggcatatcg gcacgatcga
    18601 taaccgcacg gtagacaaca tgtccggtga cacgcggcgc atcgcccaat aagctctggc
    18661 gcaccactga cttcacgccg tcacagccaa tcagaatatc gccggtccag ctattgccgc
    18721 gatcgtcaaa cacggtgaca tcgtcttccg tctcgcgaat atccacgacg ttggtggagg
    18781 tgtgatattc cacgtccgga tgcgtcagta ccgcttccca caccgaggcg tgaatatcaa
    18841 cccggtggat caccgcgtaa gggccgccga aatggtcacg aaaggcctgc ccggtttcaa
    18901 tgcacaccac ttcttcggcg ttcaccgcat ccatcatggt aatgtgatcg gtaaacaccg
    18961 cgcgctggcg ggcaacttcg ccgacgccga ggctgtccag cgccgaaaac gcatttggcc
    19021 ccaactggat gcccgcgcca atctcaccga tctcatgcgc cttttccagc agcaccactt
    19081 tgatccccag gcgggccagt gacagcgcag ttgccgcacc gccgatcccg cctcccacaa
    19141 ttactgcgcg tgtcactttc gtcattatag ttctcctggt caggctgaaa ttttatcttg
    19201 ttgattttct ggcgctgcgg caatgaacgc tggcagctgt gtgcaggcgt cgtacaccgc
    19261 tttgcagcgt ggatacccgc tcaaatcgca gttcatgcgt agcgcgttcg cccactgcgg
    19321 gacaaggcag caatctgcca gcgtcggcgt ctcgcccaca caaaattgcc ccgactggtt
    19381 ctggcgcaac agttgttcca ccgcgcttaa cccttgctgg atccagtgcg cataccaacg
    19441 ttttttttct tcttcactga cgttcagttc ttcggtcagg tagcgcagca cgcgcaagtt
    19501 attcaatgga tgaatatcac aagcgatggc atagacaatt tccagcacct tgttgcgggc
    19561 cggctcttcc tgcggcacca gcggaacttg cgggtagtgc ctctccagcc agtcaataat
    19621 cgccagcgat tgtccgagtg attcgccctc gtcagtcagc agcgtcggca ccagcccgac
    19681 cggattcatc cgccgatagg ccagttcatt ttgctgacca atacggatat tcaccccttc
    19741 ggtctggtaa ttaatgcctt tcagcgccag cgcaatgcgt acccggtacg acgctgaact
    19801 attaaaaaaa ctgtacagct tcatcatgca cctcagacaa tcttcaccgc gataggcgtt
    19861 aagccctcga cgttgcctgt gatcacatca cctttcacta ccgggccaac cccttccggc
    19921 gtaccggtga agatcagatc gcccggttgc agctcaaaga agcctgacag atagcagatg
    19981 gtttcattca ccgaccagat gagatggcga atatcgctgc gctgatgatc ttcgccgtta
    20041 acctgtaacc agattggcgc attatcaata ccgccgatat cgcgggcttt gtgcagcggc
    20101 gcgatgggcg ctgagagatc aaacgcttta ccgatttccc acgggcggcc catctggcgc
    20161 atttccattt gacggtcacg ccgagtcata tcaagacccg tggcataacc ccagacatat
    20221 tcatgggcgt gttccagcgg gatatcgctg ccttttttac caatcgccac caccagttca
    20281 atttcgtaat ggtagttatc ggtctgtgcc ggatacggca gcgccagcgt ttcaccagcg
    20341 gcaaccggaa caacggcatc cgccggtttg cagaagaaga atggcggttc acgatccgga
    20401 tcaaagccca tttcgcgggc gtgagcggca tagttacgtc cgacgcagta aacgcggcgc
    20461 acggggaatt gttcatcact gccaacaacc ggtacggtaa cgggcgcttg cggttcgaat
    20521 acgtattgag tcatttttct ctcctaaatt aataacgcgc ttcgcggaac agccccaggg
    20581 cttcttgtac cggtctgtcc gaaaaactaa ataaaacgga atcttgcgtg gtctggaacg
    20641 acacgccatg ccaggtcggc accacgaaga tatcttttgc actaaagctg aaggtttcat
    20701 tgccgatgat gacctgcccg ctaccttcca ccacatggta gatggtgctg tcagtggtac
    20761 gcgcaacgcg tgaggcaaac cctttcggca gcagttgcag gaaagcgccc atcgacggca
    20821 tcggatagcc gccggtgacc gggttgacgt agcgcatttt gtagccatcc cactcgtcgg
    20881 catcgcccag tcgggttaaa tcgtgcagca cttcgcggct gcggtcataa cgatagttaa
    20941 agatcggcga ggagttcccc gtctgatgcc gcagcggcaa catattggca gcgtaacgcg
    21001 gcagataatc tccctcttta cgcgttaccg gttgttgctc ttccggataa tcttcggcaa
    21061 aaccgcagcc cagaatattc actaatggca gatccagccc atcgagccag ataaccggtt
    21121 cgtcgccagg gttaccgtga tcgtgccagc gccactgcgg ggtcaggata aaatcgcctt
    21181 cattcattgg cgtgcgttcg ccatcaacgg cggtaaatgc ccctttgcct tcaacaataa
    21241 aacgcagcgc cgactgatta tgacgatgac tcggtgccac ttcgcccggc atgatcagtt
    21301 gcaaaccggc atataaagtc gcggtaatgg aagactgccc gcgcagcgcc gggttttcca
    21361 gcaccagcac gcggcgcacg gcctctttcg caccaatcag gccgccgctc tccagtagca
    21421 gcgggcggat ctcctgataa ttccagtacg ctggcgcaca gttagcgttg ggcgttttcg
    21481 gcaccagatg gtgcagtgac tcccacagcg gggtcaggtt ttgcccggaa atatgctgat
    21541 aaaattgctc gcggctattt tgattgttat cagtcattga gatgctcctt attcgttcac
    21601 aacgcctgcg cgaggcagtg agtcagggac agaggtcgac tggcgaacca cggtaatcag
    21661 cagggttaac atcaccgcac tgatcgccgc aggcacagcg ataatgaaaa acagggtgtc
    21721 gaaagagaaa ttcatcgcca tcatcacgcc gccagacagc gaaccaacaa tcgcgccgca
    21781 gcggccgata gcatttgacc agctcacgcc cgtggctcga ctttgtgttg gataaagtgt
    21841 tgcggtcagc gcattcagcc ccacctgcga tccgctaatg ccaatgccag tgccgaaaat
    21901 cgccagcgcc atcaaccata atccatcctg gcttaagcca atcatcacga tgcatatagc
    21961 gcctatggca tagctcagcg tcagcacgcg gaaaggattg aacttatcca tcagcacacc
    22021 cagcgccagc gcgcctaacg taccaccaat ctggaacgcg gcggtgaccc tggacgcgtg
    22081 ttgcaggtcg atgccgcgat ggttcagtag cgtcggcatc cagcttgaga gcaggtaaat
    22141 aatcagcagg ctcataaaga acactaccca cagcatcaac gtgatgggta actgacgtcc
    22201 cataaacagt tgacgaatgc tgccttttgt aacggaggct gattcaatga gataaaagtg
    22261 ggtatcgaca taacgctcgc gggtgatggc gctgacggtt ttcgcaatca ccgcctgcgg
    22321 tagctggcgg cgcacctgcc agcgtggcga ttccggcaga actaccagca gtgcgacaaa
    22381 cagcatcagc ggtaacacgc cccccaacac cagaatgccg tgccagccaa tcaccggcac
    22441 cagctgcgca ctgacaatcc cgccaaacgc cgaacccagc gtaaagccgc aaaacatcag
    22501 cgtcaccagc gcgccgcggc ggcgggcagg cagatactcc gaggtcatgg tgatggtgtt
    22561 cggcatcgcg ccgcccagac ccaggccggt gagaaagcgc aggaagacca gcgtttgcag
    22621 atccggtgag aaagccgagg cgaggcttaa cgcgccaaac aaaaagacgc acagctcaat
    22681 cacccgcttg cgaccaaagc gatcggacaa cgggccacaa agcagtgcgc ctgcggttaa
    22741 tcctagcaga cccgcgccaa aaagcggggc gagatcgcct gcggtgagtt gccagtgagt
    22801 gcgaatatca ggggcgataa atccgatagc cgcggtgtca aaaccatcga gcattaccac
    22861 cagaaaacag cagatgataa cgcgccactg catcttgccg accggagcag catcgataag
    22921 tgcttgtaaa tcacgtcgtt gagtcatagt aaatgcctca gtgcaggtaa ggtagatgtt
    22981 tcttttgttt tgtatatgtt gcgatattgt gattaaagag atgagatgta caatggcatt
    23041 tcgggcagag agataacctt gaggttatgg caaatggcga actgggcgca aaaattaaaa
    23101 ctacatcacc ttgaaatgct ggtggcgctg ggtgaacagg gcaatctaac ccatgtcgca
    23161 cgaatgatga atatcaccca acctgcgctt tcgaaatggt tgtcacaact cgaagatgag
    23221 attggaatca cactttttga gcggcacagc aaaggattac gtccgactga aggcggcaaa
    23281 ttgttattgc aacatgccca acggttaatc aatgacatgg agcgatcgca acatgaactg
    23341 gcccgtttta aacaaggtgg actggtcggc agtctgaaaa ttggctgttc tcccgtcgcc
    23401 actgactgcg tatcccaggc gattttgcat ttgcttacga agatgccaac tctacacctg
    23461 aatatcgaag agaaggtgat gaccccattg ctgcaagatc tattggccgg ttctgtcgat
    23521 gtggtcgttg ggcgaattgg cggtcgcgca ttgcagctgc cactgaatta tcaggtgctg
    23581 tacaccgagc cagtatgctt tgtcgcccgc cctgagcacc cactggcgaa atacgccacc
    23641 ctcagctgga atgatcttgc caactggcgc tggatcgtct ggccgacggg tacccccatt
    23701 cgcattagta ttgataatgc gctggtagac cacggcgtaa tgctgccaga aaacacgatt
    23761 gagtcggcgt cgatgaacgt cagtaccaac ctgttacaaa gcagcgatat gatctctatt
    23821 ttgtcattac gcctggcgca acgttatgcc agccagggac aactggtgat actcaacctg
    23881 ccaaagatag aacagaaagg tagcgtcggt gtgttctggc gcaataacga aacgccttct
    23941 tatgctttaa gtcagtttct gcacttttta cagcagaatt agtgtgctga acggggcgct
    24001 cgcccgaggt tacctcccta aaattcaact atgctgagaa ttcttaatgt gatcggtagc
    24061 acgttttacg aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa
    24121 gacactgaac attatctggc aatatttacg cgctttcgtc ctgatttatg cctgcctgta
    24181 tgcaggcatt ttcattgctt ccctgctacc ggtaaccatt ccgggcagca tcatcgggat
    24241 gctgatcctg tttgtcctgc tggccttgca aattcttccg gcgaaatggg tcaatccggg
    24301 gtgctacgta ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat
    24361 gcaatatttt gatttgctcc gcgcacagtt tggcccggta gtggtttcct gtgcaatcag
    24421 tacgctggtg gtttttctgg tggtgagctg gagttcgcaa ctggtacacg gtgaacgtaa
    24481 agtcgtaggt cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg
    24541 acgttgattg tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt
    24601 aacccgttgc tggtagcaat ggtggtgatc attccttttt taatgctgac cggcatctct
    24661 tacgacagtt actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc
    24721 gcgctggcct atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc
    24781 atcaccatct gttttatcgg cagcgtggtt gcgatggtga cggggacttc cgtggcattg
    24841 ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct
    24901 attgcaatgg cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtg
    24961 attttcgtcg gcatcctcgg cgcggtattt ggccatacat tgcttaatgc gatgcgtatt
    25021 cgtaccaaag ctgcgcgcgg tctggcaatg gggactgcct cgcacgccct cggtacggcg
    25081 cgctgcgccg agctggatta tcaggaaggt gcatttagtt cgctagcgct ggtgttatgc
    25141 gggataatta cttcgctgat cgcaccgatc cttttcccga ttattctggc agtaatgggc
    25201 taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga
    25261 gattcagatc acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta
    25321 tgaggcaacg ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg
    25381 caatctgcac tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag
    25441 caagtctcat cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta
    25501 cttccgctgg cggcggcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt
    25561 gcgcgcggtg tgagcggaac ctggtatttt ggtgccaata tggaatttat tggtgcgaca
    25621 atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa
    25681 aaagcgcttg cagccatcac cgttaactac acgccttgtg gtcactgccg tcagtttatg
    25741 aatgaactga acagcggtct ggatctgcgt attcatctgc cgggccgcga ggcacacgcg
    25801 ctgcgtgact atctggcgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg
    25861 atggacgagc aggatcacgg ctatgcgctg actggtgatg cgctttctca ggcggcgatt
    25921 gcggcggcaa accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa
    25981 tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact
    26041 ctgccaccgt tgcagggagc gttaattcta ttgaatctca agggttatga ttacccggat
    26101 atccagcgcg cagttctggc agagaaagcc gatgcgccgt tgattcagtg ggatgccacc
    26161 tccgcaacac tgaaagctct cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc
    26221 tggtgccgga tgcggcgtga acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa
    26281 atcccggcaa acagtttgtc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga
    26341 aattttcctt agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc
    26401 tgatcggctt gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg
    26461 cgccttatat ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg
    26521 gaacagcaaa atattatcgt actggcgtaa ttaatcagta ttatcgctac cgcattcaag
    26581 gagcgattaa tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg
    26641 cattgcaaag ttataatgag ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg
    26701 acccatcaga tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta
    26761 cacgcaaagt tttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc
    26821 gggcattatt tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac
    26881 cgaaagatat gctgtcagta cgtattcgtg aatttgccgc ccgtttcggt gcgctggctg
    26941 acctttatat ttttaaacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta
    27001 tgcaccaggt gccggaagat gcgcaggggt atcccgccgt cacacccgaa cagttacttg
    27061 aattacaaaa gaaacaagga aagtagttat ggatgtacag cagttttttg tcgttgccgt
    27121 ttttttcctt atcccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc
    27181 gattgataaa cgggttaaaa atgcaccgga accggtgtat gtctggcgag caaaaaatcc
    27241 cggactcttt ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat
    27301 gattgtttat cttattttct atcgttaata ttttctcttt tctgttaatt attgagaata
    27361 attattactt cactcaataa gctgcggata taacattctg aaccgcagct atttgtgaat
    27421 cctttcacag tttaaatttc cccacgcgca tagccttaat atcagcacgc aatatttatt
    27481 tagcgttcgc cttaattact catagcatta aggaagatca catgccgcaa caaaattatc
    27541 tggatgaact cactccggct tttacgccgt tactggcgat taaagaagcc tctcgctgtt
    27601 tattatgtca cgacgctccc tgtagtcagg cttgcccggc acagaccgat ccggggaaat
    27661 ttattcgctc aatctacttt cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata
    27721 atgccctcgg tgccgtttgt gccagagtgt gcccgacgga gaaattatgc caaagcggtt
    27781 gtacccgtgc cggtgttgat gctcccattg atatcggccg cttacagcgt tttgttactg
    27841 attttgaaca acaaaccggg atggaaattt atcagcccgg tactaaaacg ctcggcaaag
    27901 tcgcgattat tggcgcaggt cctgccggat tgcaggccag tgtgacgcta acaaaccagg
    27961 gttatgacgt cacgatttat gagaaagaag cgcaccccgg tggctggttg cgtaacggta
    28021 ttccgcaatt tcggttaccg caatcagtgc tggatgcaga aatcgcccgt attgaaaaaa
    28081 tgggcgtgac cattaagtgc aataacgaaa tcggtaaaac actcaccctg gagcaactga
    28141 aagcggaaaa ccgcgcggta ctggtcaccg tggggttatc aagcggttcc gggctaccgc
    28201 tgtttgagca tagcgacgtt gagattgccg tcgacttcct gctacgtgca cgacaggcgc
    28261 aaggcgatat cagcattcca caaagcgcat taattatcgg cggcggtgat gtcgcgatgg
    28321 acgtagccag cacgctgaaa gttctcggct gtcaggcggt aacttgcgta gcgcgtgaag
    28381 agttagatga gtttccggca agcgaaaaag agtttgccag cgcccgggag ctgggcgttt
    28441 cgatcatcga tggattcacg ccagtagccg tggaaggcaa taaagtcacg tttaagcatg
    28501 tacggttatc gggcgaactg acgatggcgg cagataaaat tattctcgcc gtcggtcagc
    28561 atgccagact ggatgacttt gcgaagttag agccgcagcg taacaccatc aaaacacaaa
    28621 attatcagac ccgcgatccg caagtctttg ctgctggcga tattgttgag ggtgacaaaa
    28681 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
    28741 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
    28801 caacccgttc tgcctctctt cttcgccggt aggcaactgc tatgagatgt gtgccaaagc
    28861 ctacgacaca ggttggggcg gcgtggtgtt taaaacgatc ggctttttta tcgccaacga
    28921 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
    28981 tatggaacag attgctgaac atccgttgga agagaatctg gccgccctgc gtcggctgaa
    29041 ggaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
    29101 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
    29161 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
    29221 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
    29281 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
    29341 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
    29401 tatgccgatc gttaacggaa aatcgagtat ttccggatat tccggtaaag cggtaaaacc
    29461 gatcgccctg cgcttcattc agcaaatgcg cacccatcca gaactgcgcg atttcccaat
    29521 cagcggtatc ggcggcattg aaacctggga ggatgcggcg gagtttttat tgctcggcgc
    29581 agcaacgtta caggtcacca ccggcattat gcagtacggc tatcgcatcg tagaagatat
    29641 ggcgagcggg ttatcgcatt atctcgccga tcagggattt gattcgttgc aggagatggt
    29701 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
    29761 tccccgtatc aatcttgata aatgtgttgg ctgtggacgc tgttatattt cctgttacga
    29821 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
    29881 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
    29941 ggaagtgaag tttaagaaag gcgagaaaga acacccggta acgttgtaaa accagagacg
    30001 catccggcat ttggttcctg tcgctggatg cggcttgaac gccttatccg gcctacaaaa
    30061 ccatacaaat cagtatgttg agctcattgc aggccggata aaacgcgcca gcgttgcatc
    30121 cggcatttgg ctcctgtcgc cggatgcggc gtgaacgcct tatccggcct acaaaaccac
    30181 acagatcatg atgttatgtg cgtttatgac cgaatgcgga ccacaatcat ttcacttctt
    30241 acgcgcgtac ttcagtgaat ccagcgctac ggcgaagata ataatcgccc ctttgatgat
    30301 gtactgccag tatgggttta cgccgatgta cgtcaggcca tagttgatga cggtaaaaat
    30361 aattaccccg gtcaccacgc caatcaccgt ccccacaccg ccgctgaacg atacaccgcc
    30421 taccacgcac gccgcgatag catccagttc atacataaag ccgaggttgt tggtggcaga
    30481 gccgatacgt ccggcttcta acatcccgcc aaaggcatag aacacgccag acaacgcgta
    30541 gatcatcagc aggttcaggc cgacgttgac accagatact tttgccgctt ccgggttacc
    30601 gccaatggca aaaatgttct taccgaagcg ggttttgttc cacaacaccc agacgaacgc
    30661 caccgcaatc aacgcgtaga aggtgatgta agagagacgg aaactcccca gcgcgacaaa
    30721 gccctgagca aaggtagaga agccactgtc aaaaccagaa attggcgacg ccccgacaaa
    30781 gtcgtaataa agcgagttga tgccatagac gatgatcatc gtgccgagcg tggtaatgaa
    30841 cggcgtcacg ttgagataag cgataatcag accgttgatc aaaccgatca ccgcaccaat
    30901 ggcacagaca atcagaataa ccagcgcaat cggcatcgtc gccatttccg ggaacacttt
    30961 gttggcgtta tccatggact gcaataatgt cgctgccacc actgccgcca gccctacttg
    31021 acgccccgca gaaaggtcgg tcccctgggt gacaattaac cctgccacac cgagcgcgat
    31081 aataatacgc accgatgact gggtgagaat attacttaag ttcaacagac ttaaaaatgt
    31141 tggggcctgg aaaataataa tcgcaagcaa aactaaaaga acgacgtaaa taccgccctc
    31201 tttcaggtaa gtaagaaaac ttttcttatt taacgcactc atgggaagcc cctaatctta
    31261 aaggtgcaaa gacgcaagac gtagaatttc gttttgcgtt gttgtttttg tatcgacaat
    31321 tccggaaacg aggccattgc tcataaccag aatacggtct gttatcccta acaactctgg
    31381 catttcagag gagataataa taatcccctt gcctttcttc gccagttcgg caattaactg
    31441 ataaatctca aacttcgccc caacatcaat accgcgcgtc ggttcatcga gcattaatat
    31501 ttctggttgc gttaacagcc agcgcccaat aatcaccttt tgctgattac cacctgagag
    31561 cgaaccaatt tgcgtccgat gccccggtgt ttttacccgc atcgaatcaa tcacccactg
    31621 ggtatcgcgt ttcatccgcg agttatccag taaaccaact ttatttttgt agttgcgaat
    31681 attggaaatt aacgagttaa aaccaatatc cagataggca taaattcccg ttgagcggcg
    31741 ctcctcagtt accagtgcaa atccgtggtt tatggcttcg ttggcattat ggttattgat
    31801 ctttttgccg tgcaacgtaa tggtgccagc cgatttctcg cgaataccaa ataacgtctc
    31861 aacaatatcg gtacgtttcg cccccaccag accggcaata ccgaggatct cccctttatg
    31921 cagatcaaac gagacatcgc gaatcgacgg ctggcgcagt gacgtcaggt tacgtacctc
    31981 gaggatgact tcgcccggct tgttttcttt gtcaggaaaa cgctggttaa gagaacgccc
    32041 aaccatcatg gcgataatct tgtccatcgt cagccctgcc agcggttcgg tggcgatcca
    32101 ctgaccgtcg cgcaatacgg taacttcatc acataactgg aagatttcct ccattttatg
    32161 cgagatataa acaataccgc agccgcgctc ttttaattta cgaataatag tgaacagatg
    32221 attgacctct ttttcggtta acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt
    32281 ataggaaaac gctttggcga tttcgatcat ctgcatttgc gaaacagata atgtgccgac
    32341 gcgcgcacgc ggatcgatat caatatccag ttcatcaaaa atcgctttgg tttcgcggta
    32401 cattttgtcc tgatcgacaa acatgccttt ggtggggtat cgcccaagcc acatgttgtc
    32461 catcaccgaa cgttgtaata ccaggtttaa ctcctggtgt accatcgaaa taccattttc
    32521 cagtgcttct ttggccgaat ggaaatcgat ctctttaccc tggaataaaa tggtgccgga
    32581 gtctttttta taaataccaa acaggcattt taataatgtt gattttcctg cgccgttttc
    32641 ccccattaat gcatggatag aatgtggccg gacttttaaa ttaacgttat caagtgcctt
    32701 aacaccagga aaagacttgt tgataccgct catttccaac aagtattccc cggaagacgg
    32761 agtcgttgag ctgaccatat aattttacct tgttggccat acaataaggg cgcagtaata
    32821 gactgcgccc aatcagtctt atttcttgct gaattcagcc aggttgtctt tatctacgcc
    32881 aacataaggt acgcggacca ctttgttgtc gattttccag ttggtgccat cagccgcacc
    32941 tttaccatcg gccaggtttt tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc
    33001 gttcagtacg gtgcccgcca gtgcaccgga tttcaccagc gccagcgctt ctggcagagc
    33061 atcgacgcca aacaccggaa tgctggactt gttgtgtgct ttcagtgctt ctaccgcgcc
    33121 cattgccatc gcatcgttgt tggcgataac cacttcgatt ttgttggcgt tcgggccaga
    33181 cagccaggcg tccatcttat ctttcgcctg agcggtatcc cacatagcgg tatctaactg
    33241 taactgttca gttttaatgc ctttgtcgtt cagctcttta atcacgtaag tggtacgtgc
    33301 ttctgcatcc ggatggcccg gttcaccttt cagcagtacg aattgaatct gaccgtcttt
    33361 attcagatcc caaccctgat tcgccgccca gtgtttagca atcaaatcgc cttgaataat
    33421 gccggactct ttggagtcag tgccaacgta gtaggctttg tcgtagctat ccagcgcctt
    33481 acgagacggt tctttgttaa agaataccac cggcacattt tgcccacgcg ctttctcaat
    33541 caccgtaccc gcagctgccg ggtcaaccag gttgattgcc agtgccttca cccctttcgc
    33601 cagcaatacg tcgatctgat cgttctgctt ggactggtca ttctgtgaat cattcatcag
    33661 cagctgaaca tctggcgcgg ctttcgcatc ttgctcaata gccttgcgca ctacagacat
    33721 aaagttatcg tcgtacttat agattgttac accaatgcga gtatcagcag cgtgtgcagt
    33781 ggcaccgaat aacatgctgg ccatcacagc agacagggtt aacaccttct tattcatggt
    33841 atctccggtt tttcttatgc agggtagtgc ttgagataaa tgctcggcgg ggcagtagag
    33901 ttaatgaagt gttactgaac gccgaagctc actttttaaa attcgttctt ccatgcccgg
    33961 taacgctcca gaaaacggct ttaattgttg ttatgacgca ttacctcggt gaaagtgatt
    34021 attcactgtt acatacgggt tacaacgtta aaacggtgca atcatagcta tcacattgtt
    34081 aagatactgt gaaatcactc acagattgaa agcggttaca tcgcctgatt tgttgagtta
    34141 gtgatcgccg ccgcattctg gcgtgttgcg acagaatggc gacgcactaa cgtcggcata
    34201 aaacagtggc tggcacgagg atcaatattg cctgctgccc cctgcaaggc cagttcggtg
    34261 gctaatttcg ccattgaagc aatgggataa cgcacggtcg ttaattgcgg gtcggtgtaa
    34321 cgggcgatgg gaatatcatc gaaaccgatg attgagagat gtaacggaat cgcaatgcca
    34381 ttatctttta atgctgtcag tgcgccagca gccatattgt cgttataagc aaatacagcg
    34441 gtaagttgta gattgcgccc cagcagttca accatcgccg cctcaccgcc cggcatgtcc
    34501 ggcgtaccag tgccaatcca gctttccggc ggaataatat cctgctcttt caacgcactc
    34561 atccagcctg ctttacgcat ggcgtcatct tcaatgccgt ggctggaaga aagataacca
    34621 atacgttgat gaccgttatt cagcaacatg cgcgtcgcca ttcgggcacc gctgagatta
    34681 tccaggcaaa cgcaacgatg ggtgtacccc ggcacaacgc ggttgattaa caccataccg
    34741 ggaatgttat ccataaattg cgccagttca tcgtcactca atgcttttga gtgaacaatc
    34801 aacgcattac aacgctggcg aattaacacc tcaatggcgt gacgctcttt ttccgcttca
    34861 tgatagctat tgccgattag cacgtatttc tgatgctgct gagcgaccag atccaccgct
    34921 tttaccagcg cgccgaaaaa cgcatcagaa acgtccatca ccaccacgcc aatggtgtcg
    34981 ctaacctgag ttgccagcgc ctgggcattg gcgtttggcc gataatccag ctcactcacg
    35041 gctttcatta ctgcttcacg cgtgtcggca ctgaccagcg tgctgttatt gagcacccgg
    35101 gaaaccgttg ccacagagac gccagcctga cgcgctacat cacgaatggt gatcatattc
    35161 actacctgtt atgagattgc agtaaatgac tgcttgctgc cggctatttt gtcagcactg
    35221 aatacaggac ttcgtgaatc gagtcacagc aatggaaacg gttacagccg ttttgttaac
    35281 gaatgtgatc cagatcgtta tcttgatgtt ttagatattg ccggtccggc agcacgcaaa
    35341 gttaactgac gccagagcca ttccaccggc ccctggcgga agtaacgcag ccagataaca
    35401 gagaagagaa tattcgccag ccataccgga ataacaaacg ccagcaattc cagacggtca
    35461 aactgcataa acagaccgag gtggtaaaaa agcgtggtac aaatcagcgt ttgcaataga
    35521 tagttggtca gcgccatccg tccgacgcag gcgatcgcca gcaccagctt aaagcggctc
    35581 aattgcggcc agaagccata aaacagcgac gcatagccaa tcgcctgaaa cggcgcactc
    35641 agttcccgcg gcatttgaag taagaaggca caccagcgat acgcccagtc cagctgccat
    35701 tgcagggcga tggcaggaag gttaatgatc accccaatcg ccactagcac aaaaccagta
    35761 cgacgatagt gacgtaagct gaactgccct ttcagccagc cactgcgcat caatgcggca
    35821 ccaatgagca tcatcccagc cagttgccag ccatactgtg cgcccagtgc caataaactg
    35881 ttcccaacac catcggcacg attactgatc gcttcaacgc cgccgtgaag cttccagtat
    35941 ttttcatata aaatagccga tgcatccggc gtccaggcgc ggctagtctg gctatcggaa
    36001 atcaacccca ataacagcaa aacgccaagc cccaccagat aaagcatgac gccggtatta
    36061 aaaaggcttt ttaccgatgg cgcatcgcgc accagccgcc agcagattaa gcccaccagc
    36121 ccgtaagcca gcagaatatc gccgtcccag aacaataaac cgtgaataaa gcccagcaag
    36181 actaacagcg ttaaccgcga ctggatccag cgtctgccac ggggcagcaa catttgcagg
    36241 ccagcaccaa acagcagcgc aaaaagcgtg aggaatttca cctggccgat gagatcgaga
    36301 aatgcccagg tccatgcatc ctgcggcgta atagcgccgt accaggcggg attaagataa
    36361 gccgcctttg gtagcccaaa ggcgctgatg tttaatagca ggatccccag aatggcgacg
    36421 ccgcgaacaa agtcgagcgt gacgttgcgc tccatggttc ctgcctttaa atcagttgtg
    36481 atgacgcaca gcgcgcagaa actcgtggcg cgtattctga ctggatttga acaatccacc
    36541 aagagaggtc gttgtcgtgg cactggttgc atcgcggatg ccacgcgcct tcacgcaata
    36601 atgcaccgcg tcgatagaga cagccacgtt attggtgccc agcagcgttt gtagcgcaat
    36661 gagaatttgc tgcgtcagac gttcctgcac ctgcggacgc tgggcaaaga actgcacaat
    36721 acggttaatt tttgacagac cgatcaccga atctttcggg atataagcca ccgtcgcttt
    36781 gccatcgatg gtaacaaaat ggtgttcaca ggtactggtc agagtgatat cgcgcacggt
    36841 gaccatttca tcgaccttca ttttgttttc aatgagggtg attttcggga agttggcgta
    36901 atccagaccg gagaaaattt catcgacata cattttagcg atgcgatgcg gcgtttccat
    36961 caaactgtca tcagccaggt cgagattcag cagctgcatg atttcggtca tatgaccagc
    37021 aataaggctt ttgcgcgttt cgttatccat ttcatgcacg ggcgggcgca gcggtgtttc
    37081 cagtcctcgc gcaactaacg cttcatgaac cagggccgct tctttactga gtgatggcat
    37141 ttatgatttc tcctgcaggt gtgacgcctc cgccctgcgt gggggcaaag ttattaagct
    37201 gatttacagc ctgattattg tgcgtgaggc ggcgcacata atccagtatt cacagcgata
    37261 attattgtaa ttgccgctgc ctttcatcag cagatgttaa aacatcgtta tgcaaatacg
    37321 gaagtgaaag ttactcacag cacattgaat aaacggtatg atgaagaaat tgcaaacaac
    37381 acaacaagga gccacgcatg gaaatgctcg aagagcaccg ctgttttgaa ggctggcagc
    37441 aacgctggcg acacgactcc agtaccttaa actgcccgat gacgttcagt atctttctcc
    37501 caccccctcg tgatcacacc ccgccaccag tgctgtactg gctttccgga ttaacctgca
    37561 atgacgagaa cttcaccacc aaggcgggtg cccagcgcgt ggcggcggaa ctggggattg
    37621 tactggttat gccagacacc agcccgcgcg gcgaaaaggt tgccaacgac gatggctacg
    37681 atttaggcca gggcgcaggc ttttatctta atgccacgca accgccgtgg gcgacgcatt
    37741 accggatgta tgattatctg cgtgatgaat taccggcgct ggttcagtcg caatttaatg
    37801 tcagcgaccg ctgcgctatt agcggtcatt caatgggtgg tcacggtgcg ctgattatgg
    37861 cgctgaaaaa cccgggcaaa tacaccagcg tttcggcctt tgcgccaatt gtgaatccgt
    37921 gcagcgtccc gtggggaatc aaagcgttta gccgctattt aggtgaggac aaaaatgcat
    37981 ggctggaatg ggacagttgc gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga
    38041 cgcttatcga tcagggtgat aacgatcagt ttcttgctga ccaactgcaa cctgcagtac
    38101 ttgcagaagc cgcgcgccag aaagcatggc cgatgacgct gcgtattcag ccgggatatg
    38161 atcacagtta ctacttcatc gcctctttta tagaggatca cctgcgcttc catgcgcagt
    38221 atttactgaa gtgaaagtcc gcccggttcg ccgggcatct tctcatcaga agcgataatc
    38281 cactgccata aagtaacgac gtccgtcttc gttatagctg tagtcgtcac gactgagatc
    38341 cttgtcgcca aggttcagca cgcctgcgcg cagtttgacg tctttagtca cctgccaggc
    38401 cgcgccggta ttccagatgg tataaccgcc cggtgtttta gccgtcgcgc tgtcggctcg
    38461 tttctgcccg gtatagtgac cagaaacata gaatgaccag tcttccagcg ccagcggttt
    38521 ccagtccagc gtaccgttag cggtatggaa cggcagatcg gatagcggtt tgttttcgcc
    38581 gttgctgaca tcacgaccat cgttgtaggt gtagttgagt gacagtttcc attcatcgtt
    38641 gaacggaatt ttcagctcgg tttccacgcc ctgaatacgt gctttgttaa cgttgtagta
    38701 gctaaatacc ggcacaccgt tcgctgtaaa accaacaaag ttttggtagc ccggtgcagc
    38761 attgacgtca gacgtacggc tgatgctgat acgatctttc acatcgttac ggaaaacggt
    38821 aacgctggat tcaacccctt ccagccagcc ttcttcaccc atgtagtaaa gccccagctc
    38881 ccaactttcg ctggtttctg gtttcagatc cgggctaccc acaatcttac atgcgccacg
    38941 gcaggaattg ctcgtccagt cagggctaag ttgcaacaga gaaggtgctt taaatgccgt
    39001 cgcccagccc cctttcaccg ttacggtgtc ggtggcgtta taaaccaggt aggcacgcgg
    39061 actccagtgt tcaccgtagg tttcgtgatc gtccatacgc acgccggtcg tcagcgccag
    39121 cggctcgaag atccgccatt catcttccac aaacagcgcg tactggctgg cagacgtttt
    39181 ggagctggtt cccccggtca ggttcaccgc atcgctaagt ttgtcgtgac gccattcacc
    39241 gccaaccgtg agaaactgat taatcgccgt cagcggcaac gtgtatttgc cgtcgaccgt
    39301 attgctttcg gaagttatcg ggctgctgtt gccagggttt ttgttctcga ctttctcacc
    39361 gtagtatttc agttcgctgg tgccgtaatc ccaacgccca ttatggctga cggagtagtt
    39421 ctggcgttcc aggcggtttt tgtccagcga gtcggaatca cgatcctgac ggtcgaaacc
    39481 gtatccggca gtaaaatcgt gattttgatt cggtgtccag gcaaattcga cattgccgtc
    39541 gcggctggag aatccttcaa tacgcggcgt ttctccggta tcggtggtcg ttgagttttg
    39601 cgggtcatcc ttttcacgtt ttgccaggct gccgtaagct ttcattccca gcacaccatc
    39661 aattaatggt ccactggtaa agaactgacc gttataggta tcaccgcgat cgcgatgttc
    39721 ctgaatggtg gtatcgacgg taacggtacc cgaccatttc tgaccgattt ttttggtgat
    39781 gatattcact acaccgccga gcgcatcgga accgtacagc gacgacatcg ggccacggac
    39841 cacttcaata cgttcgatgg aatcgaccgg gatccagttc agatcgaaat cattgtggcg
    39901 gaagacggca ttgcgggagt taacgcgttt accgtcgaca agaatcaggg tgtagctgct
    39961 gtccagacca cgaatactaa cgcccttacg gttatcccct tcgttcgtca gttgtacgcc
    40021 aggcacttct ttgaggacat ccttcagatt ctgtaccggt tttcgctgca ggtcttcctg
    40081 ggtaatgacg ctgatactgg cgggtgcatc tttaaggttt tgttcaacgg aagatgcagt
    40141 gacaaccatc gtttcgccat cgtcatcgac cgctaacaca ggccatgcac aagaaatagc
    40201 ggacaaacac agcccgaccc gtacgaaagg gttcaaccta aacattccat atctccatga
    40261 ggtaactacg aaaataaaat gggttatcgc tcacatcttc ttcacgtccc cttgcgtacg
    40321 gcagcatcgc ggtggactta tttgtatgca ggtgatcatc ccagaaagcc ggaccagcct
    40381 ctgatttgtc agcctttttt gattgcggcg taacgataat gcaaacgata acaattatca
    40441 atccaaatgt taaattttat atccgcatga tttgtcaggg aaatggaatt gaaaaagccc
    40501 tctcggttga gagggcttag caaggaaggg agaaacttat ttcttatcgt tctgcgggaa
    40561 cttcatttcg ctgtagcgta cgaagtgggt tcctttaatc agcttgtagc cgaaccaaat
    40621 aatcaggaac agcgggatac caatatacgt tgccgctacg ccgccccagt caatggtatc
    40681 tttcaggaac gcttcgtagt tctggcccaa agtgataatc agacacagaa tgaatgcgaa
    40741 gatcggcccc aatgggaaga aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg
    40801 caatacgtaa ccgcgacgga agcgatagtg gctaatggca atccccagcc aggcgataaa
    40861 accggtcatc ccggaggtat tcagcagcca caggtatacc gtctggttgc caaacatgga
    40921 ggtcaggaag cacagaccgg caatcaccgt cgtcgcatac agcgcattac gcggcacgcc
    40981 accacgcgac agtttagcga aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag
    41041 catacgagta gacgcataca taccggagtt acccgctgac agcaccgccg tcagaataac
    41101 tgcgttcatc accgccgcag cagagagcag acccgcgtgc tggaacacca gggtgaacgg
    41161 actaacgctg atgtctttaa catcgttacg cagcaggctc ggatcggtgt acggaataat
    41221 caggctgata atcaggatcg cgaacacata gaacaacagg attcgccaga acacctgacg
    41281 taccgcgcgt ggaatgtttt tccccggatc ttcggactcg cctgcagcaa taccgatcag
    41341 ctcggttccc tggaaagaga agccgacaat catagctacg ccgatcatcg ccgcaaaacc
    41401 accagcaaac ggcgcttcgc cgattgtcca gttgctccag cccgcaggct gcgcgccttt
    41461 gaagataccg ataatcatca gcacgccaac gatgataaag acaataactg tcgtgacttt
    41521 gatcagtgag aaccagtatt ccgcttcacc aaagccgcga acagagatgt agttcagcag
    41581 gaagataacg ccgaggaaca acgcactcca gatccagccc ggtgtatccg ggaaccacca
    41641 gctcatgacc agctgagctg caaccaggtc aacggcaata gtcaccgccc agttgtacca
    41701 gtagttccag cccagcgcga agccaaagcc ctcttcaaca tagttctgac cgtaagtggc
    41761 aaacgaaccg gaaaccggca tatatgcagc cagttcaccg agactggtca tcaggaagta
    41821 aaccatcagg ccaatcaaca tatacgagag caatgccccg cccggacctg cctgagaaat
    41881 cgttgcgcca gaggcaacaa aaagacctgt accgatggaa ccgccaatgg caatcatcgt
    41941 caggtgacgc gcctttaatt cacggcgtaa gcccggcgct tctgtggttt tagtttcgga
    42001 aaccatacaa aaatgctatc catctaaaaa atgaggcgcg attgtagcag acgattggca
    42061 atccttccgg cagaaataca cggttataag acaccttcat gatcgcccag ggattataag
    42121 taaagcagcc aatctctttt ctggcgaatg catgctgaag gcgctgcgtc acgaaatcga
    42181 cacagcacca gcatgttctt gtacagcaac ttaacgcggc acatttgcgg gatcgcaata
    42241 gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta tacgccacaa
    42301 cgtacgcatc aggcgcggca gagggaccgc aacttcactt aatgtgcctg cctgcaattg
    42361 atcttcaatc acacgtcgcg acaggcaact aattcccaac ccatgacgca ccgcatgttt
    42421 gattgcctcg gagttaccta attccatcgc catctcaaac ttcggtaaat gtgacagcaa
    42481 cagataatcg acaatctccc gcgtgccgga accgcgttca cgcaggatcc acggcgcagc
    42541 ggccagctgt tctaaggtga ccggaccacg ggccaacggc gaagtcggcg cggcgaaaac
    42601 caccagctcg tcttccagcc acggttcaga aatgatttca gtgctgtggc acggtccttc
    42661 aataaagcca atatcaacgc ggaaatccag caccgcttgc atcacgtcct ggctattccc
    42721 aacgctaagt tcaatcggca actgcggata atcatggcga taacgggcga taactgcagg
    42781 cagaatgtag ttaccgatgg tactactggc atagatacga atcgcgccgt tgtcttcgcg
    42841 aaacagttgt tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag
    42901 ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga tcaaacagtt gcacgccaag
    42961 ctgcccttcc aggtcggtca aggctgcgct cactgctgat tgcgacaacg ccagcatcac
    43021 cgacgcttgg gtggttgatc cacttttcaa tacttctgca aaaacttcca actgccggag
    43081 ggtgatgtgc atagtcgctt accacttata aagattaatt ataaatatat aatcaatttt
    43141 atttttaaac cagttagtcg taaccttatg cccggttaaa ggagagggtt atgacgaata
    43201 tcaccttaca gaaacaacat cgtacactgt ggcattttat tccggggtta gccctgagtg
    43261 cagttatcac cggggtcgcc ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt
    43321 ttagtgccct caccctcgca atcttgttgg ggatggtttt aggcaacacc atctatccgc
    43381 acatctggaa aagctgtgac ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg
    43441 gtatcattct ttatggcttc cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg
    43501 ggatcatcat tgacgtgctg acgctgtcca gtaccttcct gctggcttgc ttcctggggc
    43561 agaaagtgtt tggtctggat aagcacacca gctggttgat cggtgccggt agcagtatct
    43621 gtggtgctgc cgcggtactg gcgactgagc cggtagtgaa agcggaagcc agtaaagtaa
    43681 ccgtggctgt tgcaaccgtt gttatcttcg ggaccgtcgc gattttcctc tacccggcga
    43741 tatatccgct gatgtctcaa tggtttagtc cggaaacttt cggtatctac attggttcta
    43801 ctgtgcacga agtggcgcag gtggtggcgg caggtcatgc catcagcccg gatgcggaaa
    43861 acgcagcagt tatttccaaa atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc
    43921 tggcggcgcg tgttaaacag ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta
    43981 ttccgtggtt tgccatcttg ttcatcgtag ttgccatctt taactcgttc cacctgttac
    44041 cgcagagcgt ggtgaacatg ctggtaacgc tggatacctt cctgctggca atggcgatgg
    44101 cggcgctggg tctgaccact cacgtcagcg cgctgaaaaa agctggggcg aaacctctgc
    44161 tgatggcact ggtactgttt gcctggctga ttgttggtgg tggtgcgatt aactatgtga
    44221 ttcaaagcgt catcgcataa accactacat cttgctcctg ttaacccgct atcattaccg
    44281 ttttcctcca gcgggtttaa caggagtcct cgcatgaaat acattggagc gcacgttagt
    44341 gctgctggcg gtctggcaaa tgccgcaatt cgcgccgccg aaatcgacgc aaccgcgttt
    44401 gccttgttca ccaaaaacca acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc
    44461 gatgaattca aagccgcctg tgaaaaatat cactacacat cggcgcaaat tcttccccac
    44521 gacagttatc tgattaacct cggacatccg gtcactgaag ctctggaaaa atcgcgcgat
    44581 gcctttatag atgaaatgca gcgttgcgaa cagctggggc tttctttgct caacttccac
    44641 cctggcagcc atctgatgca gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc
    44701 atcaacattg cgctggataa aactcaaggt gtgacagcgg tgatagaaaa caccgccggt
    44761 cagggcagta acttagggtt taaattcgaa catctcgcgg cgattatcga cggcgtggaa
    44821 gataaatccc gcgtcggcgt ctgcattgat acctgccatg ctttcgctgc cgggtatgat
    44881 ttgcgtactc cagccgaatg cgagaaaaca ttcgcggatt ttgcccgtac tgtcggcttt
    44941 aagtatctgc gcgggatgca ccttaacgat gcgaaaagca cctttggcag ccgcgttgac
    45001 cgccatcata gcctcggtga aggcaatatc ggtcatgatg cgttccgctg gatcatgcag
    45061 gacgaccgtt tcgacggcat tccgctgatc ctcgaaacca tcaacccgga tatctgggca
    45121 gaagagatcg cctggctgaa agcgcaacaa actgaaaaag cggtagcctg aagatgaata
    45181 accgggaaaa ggagatcctt gcaattttac ggcgtaaccc gctgattcag cagaacgaaa
    45241 ttgcggacat gctgcaaatc agccgttcgc gcgttgcggc gcatattatg gatttaatgc
    45301 gcaaaggccg gattaaaggc aaaggttaca ttctcaccga gcaggaatac tgcgtagtgg
    45361 tggggacaat caatatggat attcgcggga tggcggatat ccgttacccg caatcggctt
    45421 ctcatcccgg tacaattcat tgctcagcgg gcggcgtggg acgcaacatc gcccacaatc
    45481 tggcgctgtt aggccgtgac gtccatttgc tttcagtgat tggcgatgac ttttatggcg
    45541 aaatgctcct ggaagaaacg cgccgcgccg gcgtgaatgt ctccggctgc gttcgtttac
    45601 atggtcaaag cacatcgacg tatctggcaa ttgccaatcg agacgatgaa accgtgctgg
    45661 cgattaacga tacccatctg ctggaacagt tatcgccgca attattgaac gggtcgcgcg
    45721 atttacttcg tcatgcgggc gtggtactgg ctgattgcaa cctgacagcc gaggcgctgg
    45781 aatgggtctt taccctcgct ggtgagatcc cggtgtttgt cgataccgtt tcagaattca
    45841 aagcgggcaa aatcaaacac tggctggcgc atattcacac cctgaaaccc actttaccgg
    45901 agctggaaat tttatgggga caggcgatca ccagcgatgc tgaccgtaat gctgcagtga
    45961 atgcgttgca tcagcaaggt gttcagcaac tgtttgttta tttgcccgat gagtcagttt
    46021 attgcagcga aaaggatgga gaacaatttt tgctgaccgc accagcgcat acgacagtag
    46081 acagttttgg tgctgacgat ggttttatgg cgggcctggt atatagcttt ctggaaggaa
    46141 acaatttccg tgacagcgcc cgttttgcga tggcctgcgc ggcaatttca cgcgccagcg
    46201 gcagcttaaa caaccctacc ctgtctgccg ataacgcgct ttcattagtg ccaatggtgt
    46261 aacaatgttg ccggattcga cgcttgaccg catcttatcc ggctcataaa tcgcacacta
    46321 cgctaaacca ataaagaacc cggcaatggt ggcactcatc aggttagaaa gtgtcgccgc
    46381 cgccagcgcg cgtaaaccaa gctgggcgat ttccggcgca cggtgtggcg caaccgcaga
    46441 aaacgccccc accaccaccc cgatagaacc aaagttagcg aaaccgcaca acgcgaagga
    46501 aataatcgcc acggttttag catccagagt gccagccgtt tgcagatagg gtgagaaatt
    46561 gagataagcg acaaattcat ttattgccag tttctgtcca atcaaactcc cggcaagatt
    46621 cgcatcactc cagtcaacac ccattaccca cgccagtggg gccagcagat aacctacaat
    46681 ggactccagc gaggcatgtt caaaaccaaa ccagccgcca acgccgccga taataccgtt
    46741 aattaacgca atgatggcga cgaatgccat cactactgtc gccacacctg cggcgatttt
    46801 cagcccggtc attgcccctg tcgcggcggc ttcaataatg ctttttggcg gtgtttcggt
    46861 gaaagagagg ttattaaaag aaacctgcga agattccgta gccgggctta gcaggcgggc
    46921 aaacaagatc cccccgggga tcgccattaa cgatgccgcc agcaaatatt ccacaggcac
    46981 gcccagtgcg gcataaccaa tcattgtcga accagcaatc gaggccatgc cgctacaaat
    47041 cgctgtaaat aattcattgc gattcagccg atcgataaag ggcttcacaa tcgccggaat
    47101 ttcgttttgc ccgaggaaaa tggtagtgac cgcgacgaat gactcaatct tgctgatatt
    47161 taatgctttc tggaatatac cgccgagaat gcgaattaaa atccccatca caccgatgta
    47221 gtagagaata ctcaccagtg cagtgacgaa gataattgcc ggtaataccc tgaaaccaaa
    47281 gataaatcct gcgccatcaa ataaggtgtc cattttcggt ccgaccagag aaccgaagat
    47341 aaatgcgcta cccgcgtcgc tgtacgccat cactttatgc acaccaaaag cgactttttc
    47401 agcgacccaa cgccctggcg gtaaccaaag cataatgccg ccaatcacga cctgtaacac
    47461 taacgccgcg ccaacggtac gcaggctgat cttcttcttg tttactgaca gtaaaaacgc
    47521 aatcgtcagc aataccacca ttcccagaac acttctcatg acatccatag tgattatctc
    47581 ttcatgtcat attgggcgac ggctttctgc ccatcagaaa tagccaggcc tgcatcttgc
    47641 gataccggcc cggcttgtta tcaacactta atgggttttg atgtagccgc gcacgcactc
    47701 ttcgactaag ccccagaacc agtcgctatc aatagtgatg ccgactttgg tattagcggg
    47761 cttgccaaga acgcccagct cgtcgcagac ggtacgccca taacaagggc cactgttgac
    47821 gtccacttcg acgtacatct cctgggtttt aatgccatca gggttaatca gataaccgat
    47881 gcaggtggcg tcgtgcaccg ggccgccagc caggccgtag ttttcgaact gggttttgag
    47941 agtgaagttc atgatgtcgc tgaacagctc tccggcaggg ccgcctgccc tttccattcg
    48001 tgcaatcacg tccggggtgc aaacggtctg gttggtgaga tcgaggccca tcatcactaa
    48061 tggaacgcca gaggtgaaca ccacgcgtgc ggcttccggg tcagcaaaga tgttgaactc
    48121 ggcagatggc gtgaagttgc ctgtaccgta agcgccgccc attagcacaa tttcacggat
    48181 tttgggcagg atcgcgggtt gcatacgcat tgccaccgcg atatttgaaa gcggaccaac
    48241 cggcaccaga gtgatatcgc catcgcttgc catcagggta tcaatgatgt atttcaccgc
    48301 atgagtgctt tctgcctggc gggtcagcgg ctcgaatacc gggccatcca gtccggtatc
    48361 accgtgaata ttatcggcaa cgatttgttg ccgcataatg ggttgtggca tccccgcata
    48421 aaccggaaca ttaatctcca gtttctggca aacattcagg ccattaatta atgttttatc
    48481 aagcgtctga ttacccgcta caatagtgat gcctaataaa tctattgccg gatgtttcgc
    48541 cgccatcatt atagcaatag catcatcatg acccggatca caatccagaa taatttttct
    48601 cttttccatt gtttatttcc tctgtttcca gttgcgttat tttttctaca gcaaagaaat
    48661 ttcgatcggg ccgatgattg aatcttaaca acagcgtacg tatgctaaat atgagaaatc
    48721 tcatagcgga taaacatcgt gaaagaaatc cacaataatg atcttaagca gcaattgatg
    48781 agtgaatccg cgtttaagga ttgcttttta acggatgttt cagccgatac gcggctgttt
    48841 cattttttag cgcgtgacta cattgtgcag gaagggcaac agccgtcctg gctgttttac
    48901 ctgacgcgag gccgcgccag gctttacgcc acgctagcta atggtcgcgt gtcgctgatc
    48961 gatttctttg ccgccccctg ttttattggt gagattgagt taatcgataa agaccatgaa
    49021 ccgcgtgcgg tacaggctat tgaagagtgt tggtgccttg cgctccctat gaaacattac
    49081 cgtccgctgt tattaaacga caccctattt ttacgaaaac tttgcgtcac cttaagtcat
    49141 aaaaattatc gtaatattgt ttctttaact cagaatcaat catttccgtt agtgaatcgc
    49201 ctggcagcat ttatattact ctcacaagaa ggtgatcttt atcacgaaaa gcatacgcaa
    49261 gcggcagagt atttaggcgt ttcttatcga catcttttat atgttctcgc gcaattcatt
    49321 cacgacggtt tattaattaa aagcaagaaa ggatatctaa ttaaaaacag aaagcagttg
    49381 tcagggctgg cactggagat ggacccggag aataaattct ccgggatgat gcagtaaaaa
    49441 ttatttgcaa tagcgcgatt gccggatgca acgcttgatg cgttttatcc ggtctacaaa
    49501 ttgagcatta cgccagacca ataaagaacc cggcaatggt cgcgctcatc aggttggaaa
    49561 gcgttgctgc tgccagcgcc cgtaaaccaa gctgagcgat ttccggtgcg cgttttggcg
    49621 aaatagccga aaatgcccca acgacaacac cgatagaacc aaagttagca aaaccacaaa
    49681 gcgcaaagga gataatcgca atggttttca cttccagcgt gccgcccgtt tgcaggtatg
    49741 gggagaaact caggtaagcg acgaattcgt taatcgccag tttctgccca atcaggctac
    49801 ccgcaaggtt ggcatcactc cagtccacac ccatgatcca cgccagcggt gccagcacat
    49861 agccaaaaat actttccaga gaagcattgg cgaagccaaa ccagccgcca attccgccga
    49921 taatgccgtt gatcagcgca ataattgcca caaatgccat taccaccgtc gccacaccag
    49981 cggcgatttt cagcccggtc atcgcaccgc tcgccgctgc ttcgataaag ctttttggcg
    50041 gcgtttcgct gaacgacaga ttttcaaacg tgacctgcga aagctcggtt gctggactaa
    50101 gaatacgtgc aaacaaaata ccgccgggga tcgccatcag cgatgccgcc aacaggtagt
    50161 caattggtac gcccattccg gcataaccaa tcatcatcga accagcaatg gacgccatcc
    50221 cgctacaaat agccgtaaac aactcgtttc gattcatgcg atcgataaac ggtttaacga
    50281 tcgccgggat ctcattttgc ccgaggaaaa tagtggtgac tgcgacaaaa gattcgattt
    50341 tgctgatgtt gagggctttc tggaaaatgc tgccaaggat gcgaatcagc agccccatca
    50401 cgccaatgta gtacagcaga ctaatgagcg cagtgacgaa gataatcgcc ggaagtacgc
    50461 gaaaggcgaa gataaaaccc gcaccgtcaa agaggacatc cattttcggc ccgaccagcg
    50521 aaccaaaaat gaaggcgcta ccagcatcac tgtaagacat cactttatga acgcctaatg
    50581 ccgcctgttc tacagcccac tttcccggcg gaaaatagag catgatgcca ccaagagcaa
    50641 tttgcagcag tagtgcggct gcaaccgtac gcaaactgat gctcttttta ttcactgaca
    50701 acagaaatgc tattgccagt aacaccacca tccccacaac acttctcatt atgtccataa
    50761 tgattttccc ttcatgccag taaacccggc gtcagcgcca ggtttcggta tgtttgatga
    50821 gtacgggcga cggctttctg cccgtcagaa attaacccgc gagacgctga tattctttgg
    50881 caatttcgct cgccagaatg gcgttgttga acacaagctg gatgttggat ttcaggctgt
    50941 caccgccggt cagctcagca acgcgcgcca gcaggaatgg cgtactttct ttaccaataa
    51001 caccctgcgc ttcagcttca gctaccgcct gatcgatcac cgcattgata gtgtgttctg
    51061 gcatcgcaaa ctgttccggg atcgggttcg ctaccacgag gccaccgttc agcccgcttt
    51121 gccatttcac cgccattgca cgggcaattt cactggcgct gtcgagacga atgctgacgt
    51181 caaacgggct ggtacggcag aaaaacgcag gcagcgcttt agtctgatag ccaattaacg
    51241 gcacaccgaa ggtttctaaa tactcagtgg ttaatcctaa atcgagaata gatttcgccc
    51301 cggcacaaac aacggtgaca ttagtatttg ccagttcttg caaatcggca gaaatatcga
    51361 aggtatgttc cgcccctcga tgaacaccac caattccgcc ggtggcaaat actttaattc
    51421 cggcaagcgc cgcaataatc atcgttgaag ccacggtggt tgcaccattt tttcctgcgg
    51481 caacgacaaa aggtaaatcg cgacgactaa ctttagtcac gttatgccct tcacgaccca
    51541 gtaattcaat ttcttctttg cttaaaccca ctttcatcac gccgccaata atggcgatag
    51601 tggcaggtac tgcgccctgt ttacgaatag tttcttctac ttcaattgcg gtctgggcat
    51661 tttgtgggaa cggcatcccg tgagaaataa tggtcgattc cagcgccaca accggttttt
    51721 tgttttttaa agcgtcctgc acttccgggg aaatttgtaa taattcaggg gaaattttta
    51781 attcagacat tctgcgttct ccactaacga tataacgttg gcaatcgata aatcggggtt
    51841 attggtgtat tcacaggaga gcgccattga cgaacatccc tgtgcgaaac gaacagattc
    51901 ggcaaacggc attccgtcta cccaacacga agcaagtccc gccatcatgg catcgcccgc
    51961 tccggtaaca ttaataacat tggttttgat cggcgcagac cagccacttt caccgctgat
    52021 atcgctgtaa taaacgccgt cgccgcccat gctcaatacc agtcggttca ggccatgttg
    52081 atggaaccag gcagcaactt ttgccacatc ttcacgccct gacagcgcaa tcccactcag
    52141 ggtttccgct tcaaggcggt ttggcttgag agtatggatt tgacttaatc gctcgcgtac
    52201 tttgacgcat ttccacgcgg aaaccggatc gacaaatacg ggtacgttgg cagcattatc
    52261 cagaatccat gccagtgcct cttcactgat attacagtcc gcgacaatga cctttgccct
    52321 ctgaataaat tcacggtgct gtgcgagata ttcagctgta atagcgttgc taatattcat
    52381 gtcatttata gcaaccagca tttcaccggt attatcgagt aatgataaat agctcgacgt
    52441 attttctccc ggcacaatca ggcatttatc gacataaacg ccagattgat tggtttgcgt
    52501 tagcagcgat tggccataaa aatcactgcc tacggcgctc agtagccagg ctttattacc
    52561 cagcaacgcc aggttttgtg caatattgcg ccctacccca ccaggcgtaa attttatttt
    52621 acctggattt gaatccgcat aatttaatga ttcatgtgaa tatccggcga catcaatatt
    52681 cgccgagcct ataattacga cataatcctt ttcgcgcata gcacgtcctc tggtaaaaaa
    52741 ttaccaaccc tgtcaatcca caaggatata gatactgcaa agacggttta aacatgtgtt
    52801 catatttaaa catgtgctca tagtatgcat gttttacgta aagtaaattg ccgccagaga
    52861 ggcaaagcgg gcagatttga gcaggctcac aaaatgcgat taaatgcctg aattacaaaa
    52921 gcgaggaatt tgtgacggga tgcacagaac atgaacacta aaaaagggca gaaaagaatc
    52981 tgcccttgag gaaataacct tagtgtttga tgaacgtttc aggtttctac tgaaagataa
    53041 aaccatttaa attcataaaa ttacattcac aaaccggtca ttctctttgc gctcatccaa
    53101 tgatacgggg cagtaaattt ctgccctgta acacaccttt tattacgcgg ctttcgctac
    53161 tgcgtccact tccggacgtt tcaggaaggc ataggccaaa cccgccacca gcgtaccggc
    53221 aataattgct accaggtaac ccagtaccgg cgtaatagcg ccagggatca gcagaacaaa
    53281 cagaccaccg tgcggtgcca tcagtttcgc accaatcgcc attgagattg cgccagtcag
    53341 cgccccacca acgatacaac acggcagcac acgcatcgga tcacgagcag caaaaggaat
    53401 tgcaccttcc gaaataaagc acagaccaag aaccagagcg gctttgccac cttcctgctg
    53461 cgctttgtca aatttgcgac gcgccaccat tgttgccaga cccattgcca gcggtggcac
    53521 catacctgcc gccataatcg ccgccatcgg gccataggtt tgagtactca gcagacccac
    53581 accgaatgcg tacgctgctt tatttaccgg accgcccatg tcagtacaca tcatgccacc
    53641 gaggatcgcc cccagcagaa ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa
    53701 cccttcgaga atgccagcaa ccggtttacc gatcaggtag atcatcgcca gaccgaccac
    53761 cagactggaa attagcggaa tgatcaggat cggtttcagc gcctccatac tctgtggcag
    53821 tttcagttgc gtactgatta acttcgcaat gtaaccagcc aggaagcccg caataatacc
    53881 gccaatgaag ccagaaccgg tgctgaccgc cagcataccg ccaatcagac ccggcgtgag
    53941 acccggacga tcggcaatgg agaaggcaat ataacctgcc agtaccggca ccatcagcgc
    54001 aaaggctgaa ccaccaccaa tctgcatcag cgccgctgcc aacgtacccg gctctttaaa
    54061 cgcttcgata ccaaaagcaa aagaaagcgc gatacacaga ccacctgcaa cgaccatcgg
    54121 cagcatgtaa gagacgcccg tcagcaagtg acggtatgcg cctgcactct cgttcttacc
    54181 ttcagaggtc gccgtttgag ctttgcccgc cggttcatac ggcgttgctt cagcaaccgc
    54241 tttatccagt tcctgcgcgg ttttcttcag cgccagaccg gtagaggtgc gatacatcgg
    54301 tttaccagca aatttcgcca gatccacttc gatatctgcc gccacaatca ccagatccgc
    54361 tgcggctact tcttcgggag tgattgcatt acccgcgcca acagaaccac gggtttcaac
    54421 tttcacccac cagccacgtt ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt
    54481 gtgtgctacg ccagtcgggc aagcagtcac cgcaactacg cgtttcggac cgctggcggc
    54541 aaccggtgct gtcgcagcga ccggcgcagt gtaaggtttc gcatggcctt tggcttcact
    54601 caggaacagc tcagggtgcg caactgcccg ggaaatatcg cccagccaga catttttacc
    54661 gttcagcgcg ctgtcattcg ggatggaatc accgagaaca atcgccattt ctgcgtcgtt
    54721 cggattgtcg atgatttcca gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc
    54781 catataggcg cgtgcctgac cgagattagc gtcaataatc agcagcgttt tcattatgcc
    54841 tctcctgctg tcagttaaaa ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg
    54901 gacgatcggt aatacccaca ttgctttgac ttaccgccag ggctgcaaca gcagtcgcca
    54961 gacgcagtgt gtgttcactg gattcacgca ttagcaagcc ataaatcagg ccaccaacca
    55021 tagaatcccc tgcgccaacg gtgcttacga catcgactga cggtggtttg gcgatccatt
    55081 cgccggaggc attaacccaa agcgcgcctt cggcacccag tgaaataacg acatgcgcaa
    55141 taccttgttc acgcagcgca tgcgcagctt caatcacatc tttcatttca ggcagtttac
    55201 ggcctgccca gatttccagc tcgcggcggt taggtttcac cagccacggt gccgctttca
    55261 aacctgctac taacgcttca cggctactat caaagataat gcaagggcac tgactacgca
    55321 ggcgagtcat ccagtcggtg aacgcttccg ggctgacgcc tgacggtaag cttccgctga
    55381 cacagaccat atcgaactga ccgagccagc tcagagaatc agtcacaaag cgttcccagt
    55441 cggcgggggt gacttcaaaa cccgagaagt tgaagtcggt cacttcgccg tctttttccg
    55501 tcagcttaac gttaattcgg gtgcgcccct gtacaacctg gaaacggttg gcaatgccca
    55561 gctcgctgaa cagttgctga aaaccatcct gattgtcttt accgaggaag ccgccaacgg
    55621 tgacatcaat tcccaggtct tttaatactt tggccacgtt gatgccttta cccgccgcat
    55681 gcagaccggt ggttttcacc aggttcactt cgccgcgttc aatttccggg cagaaaccaa
    55741 caaggtcata agccggatta agggtgatag tagcaacacg tctgctcatt atgcgccctc
    55801 cccaagacca gcagcgatag cgtcgccgat tgctttcagc gcctgttcag catctgcacc
    55861 ctgggcggta aagcgtaggc gatgaccttt cttaacgcca agtgccacaa ctttcatcag
    55921 actacgtccg tttgccggtt tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt
    55981 aaattgttta atggtgttga ccagcatggt acctggacga gcatgcaggc cgtgttcatt
    56041 gcgcaccaca aactccgcgc ttaacacgtc gtcggtcggc gcatcatcgc tggtcagcag
    56101 cgccagcaac gttgccgcat ccgctttcag caagcggtca gctttattgt cgagcaacaa
    56161 atcagcgaga cgcttaagaa ccgcaatggg ctgatcgtca ttcatcgcca cactcaccag
    56221 catggctgcc gtttcgccgt ccacatcaaa agcatttgcc gcacggctta ccgcaattgc
    56281 gctacgcaga ttgccttcgg cgctatcgct cagccagata ccctgtccga ggttcagcgg
    56341 ttgttcattg atggctttgg tgacgaaagt ggcgtcaact gcccccgcct ctttcagacg
    56401 cgcagcgttc agcgcctgaa gagtcagcag atcgctggcg acgatatcca gcgtcagcat
    56461 ttcgttgtcg agcttcagct gctcactctg cttttcgccc atcagcaatg cgcgaagttc
    56521 ttctgctgtt gttgctgact tcagttgttc agcaacggaa tcatcgctca gtacgtgggt
    56581 cagctggcgt agcaggccca gatgctcatc cgagctggca gcaataccga tcgctacgta
    56641 cgctacctga ccgtcacccc aggtgacgcc ttccgggaac tgaaatacct gaacgccggt
    56701 tttcagcacc tgatcgcggg tgtcggtagt gccgtgtgga atagcaatac cattgccgag
    56761 gaacgttgag gtttgctgtt cgcgcgccag catgccattg acgtagcctt ctgctacatt
    56821 accggcctgc accagcgccg cagcgacctg gcgaatcgcc tcttctttgt ctccggcctt
    56881 ttcgcccgga tggatgtcct gtacggataa ctggaacata gttctcctct cttgctgaat
    56941 tgaaacgatt cagcttctat gagaaaaaaa gcgccaacct ggcttagggt taaagacaag
    57001 atcgcgctga aacgtttcaa gaaagcataa tacttctgtt tcagcacgca aggaaagccg
    57061 taaagttatg agcaaaagtt tgatgtgctg cacaattttt tgtcattttt cctaattgct
    57121 gatgggaaaa tccgttatca gcaatttcat ttcagcagcg tcagcagcca atcagcaaca
    57181 gtgaaatggc attttgatta ttcgtggcgc ttttgatttc gctgtctgtt tattttctga
    57241 caagcagcgt aaactccgcg tcttcctctt ccagtgatcg accagcatgc ataactcccc
    57301 cgcagtctcc agcgcgaaat cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt
    57361 tctcaccggt attgcgggcg ctctgcaaac cccgacactc agtatttttc ttaccgatga
    57421 agtacatgcc cgtccggcga tggtgggatt cttctttacc ggcagcgctg tcattggggt
    57481 tctggtgagt cagtttctcg ccgggcgctc tgataagcgc ggcgatcgca aatcgctgat
    57541 tgtcttttgc tgcctgttag gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta
    57601 ctttgttttg ctattcgttg gcgtctttct tagcagcttt ggctcgaccg ctaacccgca
    57661 aatgtttgcc cttgcccgtg aacatgccga caaaaccgga cgtgaggcgg tgatgttcag
    57721 ctctttttta cgcgctcagg tttcactggc atgggtcatt ggcccaccgc tggcttatgc
    57781 cttagcgatg ggtttcagct ttacggtaat gtatctgagc gcagcggtag catttattgt
    57841 ttgcggcgtg atggtgtggc tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac
    57901 cggcacggtt gaagcgccgc gccgtaaccg tcgcgatacg ctgctgctgt ttgtcatttg
    57961 tacattgatg tggggctcga acagcctgta catcatcaac atgccgctat ttattatcaa
    58021 cgaactccat cttcccgaga aactggccgg tgtgatgatg gggaccgccg ccgggctgga
    58081 aatcccgacc atgttgattg ccggatattt cgccaaacgt ctgggtaagc gtttcttaat
    58141 gcgcgttgct gccgtgggtg gcgtctgttt ttacgcagga atgctgatgg cgcattctcc
    58201 tgtcattctg ttgggcttgc agctgctaaa tgctattttt attggcattc tgggcggtat
    58261 cgggatgctc tattttcagg atctgatgcc cggtcaggca ggttcagcca ccacgctcta
    58321 taccaacacg tcgcgcgtgg gctggatcat cgcgggatca gtggcgggca tcgtcgccga
    58381 gatctggaat tatcacgctg tgttctggtt tgcgatggtg atgattatcg ccactctgtt
    58441 ttgcttactg cggattaaag atgtttaagg cgcggtcagc atttccagat cgataagcca
    58501 ggtcattgcc tgctgacgcg agttaccgca catttcagcg ctggcttgta agcctgcaca
    58561 gactttcggc cgcagtggcg aggtgaaaat tttacagcgt tgctgttcat caagctgaat
    58621 gcagggcgta ttggcgggct tgccatctgg cataccggga atagggctgg aaattgaagg
    58681 ggcagtgcaa cacgccccgc aacccggacg gcattccata cgttgttctc ttaaggttaa
    58741 ccaccggatt gatgagcgcg cacagtacca ccttttttgc accagcaaaa gtgcgaatac
    58801 cacttgcccg aaaggctcgt cgcgagtact ttgtcgcgat atttttgaca ttttcgacta
    58861 caggaatttt tcgatgccaa gagcgaacga aattaaaaaa ggtatggtac tgaattacaa
    58921 cggcaaactg ctgctggtta aggacattga tattcagtcg cccactgccc gcggtgccgc
    58981 tacgctgtac aaaatgcgtt tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt
    59041 caaaggagat gatatcgttg acaccgtgac gctgacccgc cgttacgttg acttctccta
    59101 tgtcgatggc aacgaatatg tctttatgga taaagaagac tataccccgt ataccttcac
    59161 caaagatcag attgaagaag agttgctgtt tatgccagag ggcggcatgc cggacatgca
    59221 ggtgctgacc tgggacggtc aactgctggc gcttgagctg ccgcagaccg ttgatctgga
    59281 aatcgttgaa accgcaccag gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc
    59341 gacattgagc actggtctgg tgattcaggt accagaatac ttaagcccgg gcgaaaaaat
    59401 tcgtatccat atcgaagaac gccgttatat ggggcgtgct gactaacttc agccgcatgc
    59461 agaaaaggga tagctcaggc tgtccctttt ttaatttatt ataccaatct tctattttgc
    59521 gcttcctgaa caccttacgc cctcaatttt cactcgttga ttgatcgccc tcacacttca
    59581 tcgcattaac aatccagacc aatttcaatt gctgtcatat aactttacac tgtcattgtt
    59641 aattaatcgt tactaagaca tgactcctat gaatacaatc gcctccgtta cgctcccgca
    59701 tcatgtacac gctccacgct acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg
    59761 ctttggagcc tttcaccgcg cacatcaggc gttactgacc gatcgtgtgc tgaatgccca
    59821 gggcggcgac tgggggatct gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca
    59881 gctccgcgca cagaaccatt tatataccgt gctggagaaa ggggcggacg gcaatcaggc
    59941 gataattgtc ggtgccgttc acgaatgcct gaatgcaaaa ctggattcct tagcggcaat
    60001 tattgagaaa ttttgcgagc cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg
    60061 ctattgtatt gacccggcca ccggtgcact cgacaccagc aatccgcgga ttattcacga
    60121 tctacaaacc cctgaagaac ctcactccgc accgggtatt ctcgtcgaag cactgaaacg
    60181 ccgccgtgag cgcggcctta caccgtttac cgtgctctcc tgcgacaata ttcccgacaa
    60241 tggccatgtg gtgaaaaacg cggtgctggg aatggcagaa aaacgttcgc cagaactcgc
    60301 cgggtggata aaagaacacg tcagttttcc gggaaccatg gtcgaccgca ttgttccggc
    60361 tgcaaccgac gaatcactgg cagaaatcag ccagcatctg ggggtaaatg atccctgcgc
    60421 gattagctgc gaaccgttta tccagtgggt ggtggaagat aacttcgtcg ctgggcgtcc
    60481 tgcctgggaa gtcgcaggtg tacaaatggt gaatgatgtc ctgccatggg aagagatgaa
    60541 actgcggatg cttaatggca gccactcttt tctcgcttat ctgggttacc tctcaggatt
    60601 cgcccatatc agtgattgca tgcaggatcg cgcatttcgc catgccgcca gaacattaat
    60661 gctggatgag caagcgccga cactgcggat taaagatgtc gatttaacac aatatgcgga
    60721 taagttaatt gcacgttttg ctaatccggc gctgaaacat aagacctggc aaatcgcgat
    60781 ggatggcagc cagaaattac cgcaacgcat gctggcaggt attcgcatac atctggggcg
    60841 cgaaacggac tggtcgttgc tggcattagg cgttgcaggc tggatgcgtt acgtcagcgg
    60901 cgttgatgat gccggaaatg ccattgatgt tcgcgatccg cttagcgata aaattcgcga
    60961 tcttgttgcg ggcagcatca gtgagcaacg cgtcaccgcc ctgctttccc tgcgtgaagt
    61021 tttcggtgat gatctgccag ataacccgca ttttgtgcag gccatcgaac aagcccggca
    61081 acaaatcgca caattcggcg cgcatcaggc gctattaaac accctcaaaa tttaacgatt
    61141 tctgcggtta aagcggatga agctcgcctt cgtccgctct ccccttctct tttctgccgt
    61201 ttttagccag gattaacgct cagttaactt accagagtat tgcggagctg ttgtgaccag
    61261 gaccaacctc atcaccggtt ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt
    61321 agcccataaa gatcccaacg aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg
    61381 aattgatggt gctttgctcg ccgatagcgg cgcactgctg aaagagatcc ccggcggctg
    61441 catgtgctgc gttaatggtt tacccatgca ggtagggttg aataccttac tgcgtcaggg
    61501 aaaaccagac cgcttgttga tagagccgac tgggctgggc catccgaaac agatcctcga
    61561 tctgttaacc gcaccagtct atgaaccgtg gatagatctg cgcgccacgt tgtgcattct
    61621 cgatccacgc ctgctgctgg acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct
    61681 ggctgccgca gacatcattg tcgccaataa atccgaccgt gcgacacctg aaagcgagca
    61741 agcgctacag cgctggtggc agcaaaatgg tggcgatcgg caattaattc acagtgagca
    61801 tgggaaagtt gccggtcatc ttctggattt gccgcgtcac aatttagccg agttgcccgc
    61861 cagcgccgcg cattctcatc agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc
    61921 agagcatcaa cgctggcgtc gcagtctgaa cagtggacaa ggacatcagg cctgcggctg
    61981 gatattcgac gctgatacgg tattcgacac cattggcatt ctggaatggg cgcgacttgc
    62041 accggtggaa cgcgtcaaag gcgtgctgcg tattcccgaa gggctggtgc gaatcaaccg
    62101 tcagggcgat gacctgcaca ttgaaacgca aaacgttgcg ccaccggaca gccgtattga
    62161 gctgatttcc agcagcgaag ctgactggaa tgccttgcag agcgcgctgt tgaagcttcg
    62221 tttagcgact accgcgtaag gttgcctgcg tttttcagta agataattag agaaaatatg
    62281 attaaaaatt tgccgcaaat agtgttgttg aatattgtcg gcctcgcgct gtttctttcc
    62341 tggtatattc ccgttaatca tggattctgg ttgccgattg atgcggatat tttttatttc
    62401 tttaatcaga aactggtcga aagtaaggcc tttttgtggc tggttgcatt gaccaacaat
    62461 cgcgctttcg acggttgttc actgctggca atgggaatgt tgatgctgag tttctggctg
    62521 aaagaaaacg ccccaggcag acgacgtatc gtgattattg gtctggtcat gctattaact
    62581 gcagtggtat taaaccagct gggtcaggca ttaattcctg taaaacgggc cagcccaaca
    62641 ttgactttta ccgatattaa ccgcgtcagc gaactgctct ctgtccccac gaaagatgcc
    62701 tcacgagata gctttcccgg cgatcacggc atgatgctgc ttattttttc ggcattcatg
    62761 tggcgttatt tcggcaaagt tgcaggtctt atcgccctta ttatttttgt ggtttttgcg
    62821 tttcccagag taatgattgg cgcacactgg tttactgaca tcattgtcgg ttcgatgacc
    62881 gtgatattga tcggtttgcc ctgggtgttg ctgacgccat taagtgatcg attaatcacc
    62941 ttttttgaca aatcactacc gggaaaaaac
//
LOCUS       DBFIFS010000007        56238 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.007, whole genome shotgun sequence.
ACCESSION   DBFIFS010000007 DBFIFS010000000
VERSION     DBFIFS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.007"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..832)
                     /gene="corA"
                     /locus_tag="N7A07_000414"
     CDS             complement(<1..832)
                     /gene="corA"
                     /locus_tag="N7A07_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU2475156.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     G"
     gene            complement(984..1040)
                     /gene="ysgD"
                     /locus_tag="N7A07_000415"
     CDS             complement(984..1040)
                     /gene="ysgD"
                     /locus_tag="N7A07_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU2475157.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1202..1966
                     /gene="yigE"
                     /locus_tag="N7A07_000416"
     CDS             1202..1966
                     /gene="yigE"
                     /locus_tag="N7A07_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU2475158.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            2049..2951
                     /locus_tag="N7A07_000417"
     CDS             2049..2951
                     /locus_tag="N7A07_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HGU2475159.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(2996..5158)
                     /gene="uvrD"
                     /locus_tag="N7A07_000418"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2996..5158)
                     /gene="uvrD"
                     /locus_tag="N7A07_000418"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU2475160.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(5242..5958)
                     /gene="yigB"
                     /locus_tag="N7A07_000419"
     CDS             complement(5242..5958)
                     /gene="yigB"
                     /locus_tag="N7A07_000419"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU2475161.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5958..6854)
                     /gene="xerC"
                     /locus_tag="N7A07_000420"
     CDS             complement(5958..6854)
                     /gene="xerC"
                     /locus_tag="N7A07_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU2475162.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6851..7558)
                     /gene="yigA"
                     /locus_tag="N7A07_000421"
     CDS             complement(6851..7558)
                     /gene="yigA"
                     /locus_tag="N7A07_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HGU2475163.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQALANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7555..8379)
                     /gene="dapF"
                     /locus_tag="N7A07_000422"
     CDS             complement(7555..8379)
                     /gene="dapF"
                     /locus_tag="N7A07_000422"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU2475164.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8416..8619)
                     /gene="yifL"
                     /locus_tag="N7A07_000423"
     CDS             complement(8416..8619)
                     /gene="yifL"
                     /locus_tag="N7A07_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2475165.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8778..8920
                     /locus_tag="N7A07_000424"
                     /pseudo
     CDS             8778..8920
                     /locus_tag="N7A07_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9082..9402
                     /gene="cyaY"
                     /locus_tag="N7A07_000425"
     CDS             9082..9402
                     /gene="cyaY"
                     /locus_tag="N7A07_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU2475166.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(9442..11988)
                     /gene="cyaA"
                     /locus_tag="N7A07_000426"
     CDS             complement(9442..11988)
                     /gene="cyaA"
                     /locus_tag="N7A07_000426"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU2475167.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12375..13316
                     /gene="hemC"
                     /locus_tag="N7A07_000427"
     CDS             12375..13316
                     /gene="hemC"
                     /locus_tag="N7A07_000427"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU2475168.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            13313..14053
                     /gene="hemD"
                     /locus_tag="N7A07_000428"
     CDS             13313..14053
                     /gene="hemD"
                     /locus_tag="N7A07_000428"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU2475169.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            14075..15334
                     /gene="hemX"
                     /locus_tag="N7A07_000429"
     CDS             14075..15334
                     /gene="hemX"
                     /locus_tag="N7A07_000429"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU2475170.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPAPAPQADTPAAAPQGE"
     gene            15337..16533
                     /gene="hemY"
                     /locus_tag="N7A07_000430"
     CDS             15337..16533
                     /gene="hemY"
                     /locus_tag="N7A07_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU2475171.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17212..18867
                     /gene="aslA"
                     /locus_tag="N7A07_000431"
     CDS             17212..18867
                     /gene="aslA"
                     /locus_tag="N7A07_000431"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HGU2475172.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(19035..20270)
                     /gene="aslB"
                     /locus_tag="N7A07_000432"
     CDS             complement(19035..20270)
                     /gene="aslB"
                     /locus_tag="N7A07_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU2475173.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(20417..20493)
                     /locus_tag="N7A07_000433"
     tRNA            complement(20417..20493)
                     /locus_tag="N7A07_000433"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20457..20459),aa:Pro,seq:tgg)
     gene            complement(20536..20622)
                     /locus_tag="N7A07_000434"
     tRNA            complement(20536..20622)
                     /locus_tag="N7A07_000434"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20586..20588),aa:Leu,seq:cag)
     gene            complement(20643..20718)
                     /locus_tag="N7A07_000435"
     tRNA            complement(20643..20718)
                     /locus_tag="N7A07_000435"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20683..20685),aa:His,seq:gtg)
     gene            complement(20777..20853)
                     /locus_tag="N7A07_000436"
     tRNA            complement(20777..20853)
                     /locus_tag="N7A07_000436"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20817..20819),aa:Arg,seq:ccg)
     gene            complement(20956..22341)
                     /gene="yifK"
                     /locus_tag="N7A07_000437"
     CDS             complement(20956..22341)
                     /gene="yifK"
                     /locus_tag="N7A07_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2475174.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(22532..23272)
                     /gene="wecG"
                     /locus_tag="N7A07_000438"
     CDS             complement(22532..23272)
                     /gene="wecG"
                     /locus_tag="N7A07_000438"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU2475175.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(23275..24627)
                     /gene="wzyE"
                     /locus_tag="N7A07_000439"
     CDS             complement(23275..24627)
                     /gene="wzyE"
                     /locus_tag="N7A07_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU2475176.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(24624..25703)
                     /gene="rffT"
                     /locus_tag="N7A07_000440"
                     /gene_synonym="wecF"
     CDS             complement(24624..25703)
                     /gene="rffT"
                     /locus_tag="N7A07_000440"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU2475177.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(25700..26950)
                     /gene="wzxE"
                     /locus_tag="N7A07_000441"
     CDS             complement(25700..26950)
                     /gene="wzxE"
                     /locus_tag="N7A07_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU2475178.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(26952..28082)
                     /gene="rffA"
                     /locus_tag="N7A07_000442"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(26952..28082)
                     /gene="rffA"
                     /locus_tag="N7A07_000442"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU2475179.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(28087..28761)
                     /gene="rffC"
                     /locus_tag="N7A07_000443"
                     /gene_synonym="wecD"
     CDS             complement(28087..28761)
                     /gene="rffC"
                     /locus_tag="N7A07_000443"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU2475180.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(28739..29620)
                     /gene="rfbA"
                     /locus_tag="N7A07_000444"
     CDS             complement(28739..29620)
                     /gene="rfbA"
                     /locus_tag="N7A07_000444"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU2475181.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(29639..30706)
                     /gene="rffG"
                     /locus_tag="N7A07_000445"
     CDS             complement(29639..30706)
                     /gene="rffG"
                     /locus_tag="N7A07_000445"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU2475182.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(30706..31968)
                     /gene="wecC"
                     /locus_tag="N7A07_000446"
     CDS             complement(30706..31968)
                     /gene="wecC"
                     /locus_tag="N7A07_000446"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU2475183.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(31965..33095)
                     /gene="wecB"
                     /locus_tag="N7A07_000447"
     CDS             complement(31965..33095)
                     /gene="wecB"
                     /locus_tag="N7A07_000447"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU2475184.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(33151..34197)
                     /gene="wzzE"
                     /locus_tag="N7A07_000448"
     CDS             complement(33151..34197)
                     /gene="wzzE"
                     /locus_tag="N7A07_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU2475185.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(34209..35312)
                     /gene="wecA"
                     /locus_tag="N7A07_000449"
     CDS             complement(34209..35312)
                     /gene="wecA"
                     /locus_tag="N7A07_000449"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU2475186.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(35552..36811)
                     /gene="rho"
                     /locus_tag="N7A07_000450"
     CDS             complement(35552..36811)
                     /gene="rho"
                     /locus_tag="N7A07_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU2475187.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(36896..36997)
                     /gene="rhoL"
                     /locus_tag="N7A07_000451"
     CDS             complement(36896..36997)
                     /gene="rhoL"
                     /locus_tag="N7A07_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU2475188.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(37138..37467)
                     /gene="trxA"
                     /locus_tag="N7A07_000452"
     CDS             complement(37138..37467)
                     /gene="trxA"
                     /locus_tag="N7A07_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU2475189.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            37598..38863
                     /gene="rhlB"
                     /locus_tag="N7A07_000453"
     CDS             37598..38863
                     /gene="rhlB"
                     /locus_tag="N7A07_000453"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU2475190.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            38999..40483
                     /gene="gppA"
                     /locus_tag="N7A07_000454"
     CDS             38999..40483
                     /gene="gppA"
                     /locus_tag="N7A07_000454"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU2475191.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(40530..42551)
                     /gene="rep"
                     /locus_tag="N7A07_000455"
     CDS             complement(40530..42551)
                     /gene="rep"
                     /locus_tag="N7A07_000455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU2475192.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            42768..43217
                     /locus_tag="N7A07_000456"
     CDS             42768..43217
                     /locus_tag="N7A07_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU2475193.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            43416..43697
                     /gene="ppiC"
                     /locus_tag="N7A07_000457"
     CDS             43416..43697
                     /gene="ppiC"
                     /locus_tag="N7A07_000457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU2475194.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(43743..45218)
                     /gene="ilvC"
                     /locus_tag="N7A07_000458"
     CDS             complement(43743..45218)
                     /gene="ilvC"
                     /locus_tag="N7A07_000458"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU2475195.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            45368..46261
                     /gene="ilvY"
                     /locus_tag="N7A07_000459"
     CDS             45368..46261
                     /gene="ilvY"
                     /locus_tag="N7A07_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU2475196.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(46313..47857)
                     /gene="ilvA"
                     /locus_tag="N7A07_000460"
     CDS             complement(46313..47857)
                     /gene="ilvA"
                     /locus_tag="N7A07_000460"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU2475197.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(47860..49710)
                     /gene="ilvD"
                     /locus_tag="N7A07_000461"
     CDS             complement(47860..49710)
                     /gene="ilvD"
                     /locus_tag="N7A07_000461"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU2475198.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(49775..50704)
                     /gene="ilvE"
                     /locus_tag="N7A07_000462"
     CDS             complement(49775..50704)
                     /gene="ilvE"
                     /locus_tag="N7A07_000462"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU2475199.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(50724..50987)
                     /gene="ilvM"
                     /locus_tag="N7A07_000463"
     CDS             complement(50724..50987)
                     /gene="ilvM"
                     /locus_tag="N7A07_000463"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU2475200.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(50984..52630)
                     /gene="ilvG"
                     /locus_tag="N7A07_000464"
     CDS             complement(50984..52630)
                     /gene="ilvG"
                     /locus_tag="N7A07_000464"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU2475201.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(52633..52683)
                     /gene="ilvX"
                     /locus_tag="N7A07_000465"
     CDS             complement(52633..52683)
                     /gene="ilvX"
                     /locus_tag="N7A07_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU2475202.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(52770..52868)
                     /gene="ilvL"
                     /locus_tag="N7A07_000466"
     CDS             complement(52770..52868)
                     /gene="ilvL"
                     /locus_tag="N7A07_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU2475203.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            53221..54741
                     /gene="yifB"
                     /locus_tag="N7A07_000467"
     CDS             53221..54741
                     /gene="yifB"
                     /locus_tag="N7A07_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU2475204.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(54765..55103)
                     /gene="maoP"
                     /locus_tag="N7A07_000468"
     CDS             complement(54765..55103)
                     /gene="maoP"
                     /locus_tag="N7A07_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU2475205.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            55222..56061
                     /gene="hdfR"
                     /locus_tag="N7A07_000469"
     CDS             55222..56061
                     /gene="hdfR"
                     /locus_tag="N7A07_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU2475206.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(56157..56232)
                     /locus_tag="N7A07_000470"
     tRNA            complement(56157..56232)
                     /locus_tag="N7A07_000470"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56197..56199),aa:Trp,seq:cca)
BASE COUNT        13736 a        15230 c        14337 g        12935 t
ORIGIN      
        1 tcccgtagct ggaagcaacc agcgttggcg gcaggaacac gacggatacc accgagaaaa
       61 ttttgatgat gcggttctgc tcgatgttga taaaacccat tgccgcctgc atcaggaagt
      121 tcaccttctg gaacagggat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt
      181 cacgcgcctg ctccagttgc ccacccggta agcgtgcttt acgtaccagg aagttgagcg
      241 cgcgctgggt atccatcaga cacaggcgca ctttccagcc gatatcttcc agttccgcca
      301 gagtggagag cgcctcgtcg tactcatcgc cctgatgccc ttccataatc acccggctca
      361 attgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt
      421 cgaacagatc cagcagcaac tcgtaggcgt taccgtctac catcgactgg ctacgggcac
      481 gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat
      541 cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga
      601 agaaggagtg aatatgcagg ccgtcatcgt cttcaaagaa acgtgccgat gcttcgatgt
      661 cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtacg cgcagtcgct
      721 cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt
      781 cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga
      841 ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag
      901 accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag
      961 gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag
     1021 tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg
     1081 ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc
     1141 gctttttggt actcgtcaag gtcgctaaca ggataaaaaa atcccacatc caggacctat
     1201 aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt
     1261 tctcgccctc accttacttc ccttgtttgc tgttgccgct gatgattgcg cactctcaga
     1321 tccaacgctg accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta
     1381 ctggcaaaaa gccaatggcg aagcgtgggg aactttacat gctctgctgg cggatatgaa
     1441 tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc
     1501 gctcggtttg tacatcgaaa acggtcagca gaaggtggcg ttaaatctcg cttcaggtga
     1561 agggaatttc tttatccgtc ctggcggcgt gttttatgtc gcgggagata aagtcggcat
     1621 cgttcgtctg gatgccttca aaaccagtaa agagattcag tttgcggtgc agtcagggcc
     1681 aatgttgatg gaaaacagtg taattaatcc gcgtattcat cccaacgtcg cctcacgtaa
     1741 aattcgtaac ggtgttggaa ttaataaaca tgggaatgcc gtgtttttgt tgagccagca
     1801 ggcgacaaat ttttatgatt ttgcctgtta tgccaaagcg aaactgaacg ttgagcagct
     1861 gctttatctt gatggcacga tttcacatat gtatatgaaa ggcggggcaa tcccgtggca
     1921 acgctatcct tttgtcacca tgatttccgt cgagcggaaa ggttaagcat tgtacactcg
     1981 aggaggccag acaaactggg gctggctaca cagctggaaa atcatcagat aaatgaaggg
     2041 aaacaaaaat gaaaaaaacg ctcgcaacgt tatttttatt aacgtgtctg ggatcatcat
     2101 ccgcttatgc ggataacgct ttgattttac aaactgattt tagcctgaaa gatggtgccg
     2161 tctcggcgat gaaaggggtc gcattcggtg ttgaccataa tcttaaaata tttgatttaa
     2221 cacacgaaat tccaccgtat aacatctggg aaggcgctta ccgcctgtat cagaccgcca
     2281 gttactggcc gcaaggttcg gtatttgtca gtgtggtcga tccgggcgta ggcactgacc
     2341 gtaaatcgat agtgctgaaa accaaaaacg gtcaatactt cgtttcaccg gataacggta
     2401 cgctcacttt ggtggcggaa tcgctgggta ttgagagcgt ccgggaaatc gacgaaaaag
     2461 ctaaccgcct gaaaggttct gaaaagtcat ataccttcca cggtcgtgac gtgtatgcct
     2521 acaccggtgc acgtctggct tccggggcga tcacgttcga gcaggtgggg cctgagcttc
     2581 ctgcaaaggt tgtcgaactt tcttatcaga aagcaaaagc aacaaaaggt gaagtgaagg
     2641 gcaatatccc gattctcgat attcagtacg gcaacgtctg gagcaatatc agcgacgagt
     2701 tgctgaatca ggcggggatc aaactcaacg acacgttgtg tgtgaccatt tccgaaggtt
     2761 cccggcaaaa atacgcaggg aaaatgccgt atgtcgcaag cttcggcgat gtgccggaag
     2821 gacagccgat ggtgtatctg aacagcttat tgaatgtttc cgtggcgctg aatatggata
     2881 atttcgcgca gaaacatcag gttgcgtctg gtgctgactg gaatattgat gtgaagaaat
     2941 gtgataagta aggtgctgat gccggatgcg gtgtggcgca ccgcatcccg caacgttata
     3001 ccgtctccag ccgggcgtat gccgccacca gccatttaat accctggccc tggaatgcca
     3061 cctgcaaacg gctatgctcg ccgctgcctt ccatattgac gatagtgcct tcaccaaact
     3121 tagcgtggcg tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac
     3181 ccatacgctg atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac
     3241 actcttccgg caactcaccg ataaagcgcg acgggcgatg gtaaacctct ttgccataca
     3301 gacggcgagt ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acaccaacgt
     3361 aggccagacg gcgttcttct tccagacgtc cgccttcatc cagcgacatc tggcttggga
     3421 acatgccctc ttccataccg acgataaaca cctgcgggaa ctccaggcct ttcgccgagt
     3481 gcagcgtcat caactgcact gcatcctgcc aggtatccgc ctgcccctcg cccgcttcca
     3541 gcgccgcatg ggagaggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc
     3601 tgaattggcg cgttgccgtc actagttcct ctaagttttc gatacgcgtc tgaccttttt
     3661 cgcccttctc ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct
     3721 gtacatgcag cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc
     3781 gctgtaaggc gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg
     3841 cctgccagag tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt
     3901 caccaatacc ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttgcggt
     3961 tggcaatcag gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca
     4021 tcccgccgta aatacggtac ggcatactgg cctgcaataa cgcctcttcg agcacccgcg
     4081 actgggcgtt gctgcggtag agaatggcgc actcggcgag cgccccaccg ttgtcctgcc
     4141 aggttttgat gcggttaacg acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga
     4201 gggaaatagg ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttgt
     4261 tgttttcaat cagggcgttc gcggcgctca gaatgttact ggtagaacga tagttctgct
     4321 ccagacggat agtttcggca ccggggaaat cattgaggaa acgctgaata ttctccacct
     4381 gcgccccgcg ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg
     4441 tgtcgcccgc cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt
     4501 ccaccaggat gttggtaaaa cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc
     4561 acaactcgtg agcgcgcagc agcagctcgg cgaagtccac caggcctgca cgatcgcacg
     4621 cttcctgata cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt
     4681 gaatatgatg cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc
     4741 gcggcggcca ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta
     4801 gctggtcttc gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg
     4861 cacgcagcaa acggtgcgcc agcccgtgga aggtgccgac ccacatgccg ccctggctgg
     4921 tgcccatcag ttgcccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca
     4981 cagccataat cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca
     5041 ccagtacgcg cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgcggcg
     5101 cggccaccgc ttcgcgctgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg
     5161 gcaccgccgc gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg
     5221 gtatatatac agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc
     5281 cagatgcggg agtaaacggc tatcccaggt ttgcatcaga tcgccatttt ccggtctgat
     5341 ccaacaggcc tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcacc
     5401 tacatgtaag atctcgccga tcggcacgtt gagtttttcc gccgccagga agtacatatc
     5461 gctgaacggt ttcgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc
     5521 caaaccaaac agttccggct gagcgttacc gttggtgatc gccaccagcg gccatttctt
     5581 cgccagctgt tttaaggtgt cgtgggtttg ctgcgggacg tcgatcctgc tgcgccattt
     5641 agcaaagttg atcattgccg cgtgtgcgcc tgcactggct tcttccgcgc tcaatccggc
     5701 gtcaagcatc gcttgttcta ttgagcgaaa acgccagcgc gtcacgtcgt gataaatctc
     5761 gggttccgct tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg
     5821 cagcgccgga tggtaatttt gcacaaaggt aagcgcctcc tgctcggtgc gcaaaatcac
     5881 cggacggtta tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa
     5941 aggccggtaa aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc
     6001 gaggcaaggt gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga
     6061 cccagcagct cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac
     6121 gagtgacgta atttatgcgg gtgaacgtga ttattcagcc cttgttttat gccccattcg
     6181 gcaaagcgtt tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc
     6241 gcgtcgtctt cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccatgacaaa
     6301 gcgttacgac caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg
     6361 ccggactcca ggtcgaggtg tttgatgtcc agccccacca gctcggaaag acgcaggccc
     6421 gcgccgtaca tcacttccag cattgcacgg tcgcgcacag cgaggggatc gttgatatca
     6481 atatccaaca ggcgatttat atcatcgacg tcgatatttt tcggcagatg acgtggcgtt
     6541 ttcggtgccg aaacaccttt cgccgggtta gctttgagtt cgttctggct gaccagccag
     6601 tcaaaaaagc tacgtagcgc agagaggcgt aatgcgaggc ttgccgcgcc cagcccttta
     6661 cggcgactgc gtacagcaaa attgcgcacc atcgctgcat cgcattgctg ccagttttgc
     6721 aggccgtttt cgctggcaaa attgatgatc gcctcaagct gtcgctggta gttaagcaga
     6781 gttatcggac taagctgccg ctccacgctc agataacgta ggtagcgttc tacatcggtg
     6841 tgtaaatcgg tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa
     6901 tttcatgaag taactgcgtt ccctgccctt gttgatagtg actggcatcg cggctggtga
     6961 acagcaccac gcctaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg
     7021 ctttcgcttc cggtagtacc accagcagct ccggtccgtt aagcggcccc agataatgct
     7081 gctcctgccc cagacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat
     7141 gagtgtggtt cgacggcgca cctaagcgcc agcgatccgg aaacaggcgc agactcgcac
     7201 ctgccaggcc gagatcacgc gcccagcggt gaaagcgcat cagcatatca tcaagactgc
     7261 tggcggcggt gagactgcgc tgcaggtaga gtaagcgata aaacaggcct tcgttggcga
     7321 gagcctgttc catcaacagc gccatgttct cttccagaac atgaatatga ttacgtgcgc
     7381 gggccatgtg ccactcgacc aacgaaacgg taccgcgtac cggatgcggc acacgtatcg
     7441 cttctactgc gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa
     7501 ccacccggtc atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag
     7561 atgaataaat ccgtcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg
     7621 acctttccag gcgatatcaa gacgaccgcc tgggagttcc acgcgtactt cttcggccag
     7681 caaaccttgc tgaatcccta ctgcaaccgc cgcacatgcg ccgctgccgc aggcctgggt
     7741 ttctcctgcc ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat
     7801 aaaaccgata ttggcgcgct ccggaaaacg ctcgtggctt tccagaacag gccccagtgt
     7861 ttctaccgcc gcagtatcga catcatcgac ctgaatcacg caatgtggat ttcccatcga
     7921 caccacgccg cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt
     7981 gttagcgcga aacggcacgg cagaaggttc gaagttgggt tcgcccatat ttacgcggac
     8041 cagatcatca tcggtgacgg taagaaccat ccgcccgttg gcggtgctga cgcggatatc
     8101 gcgcttattg gtcagccctt tcagacgcac aaaacgggca aagcagcgtg cgccgttgcc
     8161 gcactgcgcc acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg
     8221 atcatacggc ggctcaacca ccagcagttg gtcaaaccct acccccaggt gccgatcagc
     8281 taggcgacga atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat
     8341 aaaatcgttg ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg
     8401 tacagaaact gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt
     8461 tttatccggc accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcatt
     8521 tttatctgca ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag
     8581 agtaagtaat acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc
     8641 atcgctttct gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct
     8701 ttatgaagtc tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat
     8761 gttctgctgg cagtttcgtg atcaacacct cacgccagga tggctttatg cactatcgca
     8821 atgtggtttg tttgttggct tttttagttc cgtcagcgtg aggatgctcg ctcggtaaac
     8881 ctgatcataa tgtttaccag aaacagggaa aggcgtggtg taactgcgcc ctaatgaaga
     8941 gacaaatctt tcgctacaca gagacatcat tattccggtg acggatgaat catgccgtaa
     9001 cgcagccgca gaggtgcaaa ctttgaagcc ctgaccccat gccgctatac tgccgccatc
     9061 acttaaaaac aggatacaac aatgaacgac agtgaatttc atcgcctggc tgatcaactg
     9121 tggctgacca ttgaagagcg tctggacgac tgggatggcg acagcgatat cgactgcgaa
     9181 atcaacggcg gcgtgctgac cattaccttt gagaatggca gcaaaatcat tatcaaccgc
     9241 caggaaccgc tgcaccaggt atggctggca accaaacaag gcggctacca ttttgatctg
     9301 aaaggcgatg agtggatttg cgatcgcagc ggcgaaacct tccgggattt gctggaacag
     9361 gcggcgacgc agcaggcggg tgaaacagtc agtttccgct aagattgcat gccggataag
     9421 cctcgctttc cggcacgttc atcacgaaaa atactgctgt aatagcggcg tatcgtgatc
     9481 ctgattggca ggcggcatgt taccgataga ttttgtgcgg aacggaatca cctgttcacg
     9541 accatcaacc ttcacaatct gatagaactg cggcaggttg aagttgatga agcttgagcc
     9601 gtaggtaaaa cggtcatgcg atgacgagta gaagcgactg acgtcacgta ccagctcctc
     9661 tttgctgcct tcgcagtggt gatacacctc aacccgattg ctttcgtcga gaatgtagat
     9721 attaaagcca ttctcatctt gcgtttcttc gaaaaagaac tggatgatcc cttcgctggc
     9781 aaagccgtcc accaccgccg gtaatttgac gtgattggtt tcaacctgca ctgacaggcc
     9841 gtgcagtttg ttatgcgaaa tagcgccata aaactcgatg gcgttctcca gtttctgtac
     9901 cgatacattc aggcgttcga agaacaagcc ccaggtttga ccagaaacgc gcagcgcctt
     9961 gaaacgcccg gtttcctggc gggtgctgga aagacgcaat tcaatacact cagaaaccag
    10021 ttgctgcacg cgagtacgaa ttaagccgcg cagatgctgg ctataacaga agacttccac
    10081 gctatctggc ggtgcggcgt cctgatgcat tttgccgaga atagttttca gggcttcgat
    10141 catcgattgc tcgccgttga agtgcagcgt acgcacttca ttccacgagt tgcgatacag
    10201 caggtcaacg ctacctacca ggcaattttg attctcgcca aagctgaaaa catccagctt
    10261 acggaaatca aaatgcacca cctgattgcg gaacgcagct gtcgggtcat attccaggtt
    10321 aacgataatc gccagatggc ggatctcaca cgggctgtag agcgccttcg gtgtcggtgc
    10381 aggtaagcgc aacgggaaat ggtgcgacac gtcggcgacc atctcctgca acttaggcaa
    10441 atcgacaatg ccgttacctt taatatacaa gcgggtgcgc gaggtcagca ggccgttaaa
    10501 ccatgcccac gccaccagtt tattcaggta acggttatat tccagcggct gatggctgat
    10561 gatcgactca atatttggcg cgcggttata cagataccaa cctgaacggt tagcccggcc
    10621 cggcggcaca taaataaagg tcagattcgg ttccgagaga tcgggtgaaa tctgcgggtt
    10681 taccagcgtc actttacctg gtaatgcttc aaacgcggca tacagcttac gcgtcagcac
    10741 gccgatatcc tgcggactgg cggagacgct aaggttattg cgacgcgcaa agcggatcag
    10801 attacggtag ctctgcatca tcgcgtcgag caactcgttg tgcgcctcac gcacctgatc
    10861 aatcttccag ttagcgcggt tatcgagcat tgccagacga gcttcgtccc acccccactc
    10921 gctcactaac tggctcaaca ctgcgcgacg ccagcctacg caggcgcgtt cacggctgag
    10981 cttttcgcac acttttaaat agaagcagcg acgtactaaa tccagacggg tgaaatcttc
    11041 aatcgccgtc aggtattcag taacacgctc cagcatcatg cagtaaggat cgaggccaaa
    11101 cgatacaatc tcgccgtcgt gcaaacgctg tttgatatct ttcgccagca ggcgtgggtt
    11161 cgggtattcc caggaatagg cttccagcag cagtgtcttc agcaccgctt tgtatggaga
    11221 atcgatactc ttgtagagtt gccaaaggct ggcaccgaag tattcttcag cggaaagcga
    11281 gcttaagccg ccgagatcca gccattcatt tggcgtcagc acgccttgtg cgtagagcgt
    11341 cattacgtag tcatcgtaat gctcttcttc gtcgcacggc accatattcc acagaatacg
    11401 cttaccggcg agacgcacgg cggtacgata aaattcatca agcagcagta tatgctgggt
    11461 ggagccacaa tcttcgcccc ccagactgcc actctcgttg tggcgaaaac ggttttcatc
    11521 aatcaggaag aagctaactt ccacacccag cgaggcggcc cagctttcca gcaggctgca
    11581 tttacgttgt agcaactggc gctcttcgct atcgagccag gattgatgac agacccagat
    11641 atccaggtca gaggaacaac tttgccctac ggacgaggtg ctgcccatgg tgtatacacc
    11701 agtaattgga agctcacctt tcggcgggtc ctgtactgac attccacgat acagttcaag
    11761 ctcgttcagg tagtggcgtt gagtttcatc aggcgtgtaa aggcaaatgc ctttgggaac
    11821 gttaccatca aggtaacccg gcattagcgg atggtgatag tgcaacaatg tcggcagtag
    11881 actgtagacc tgttggaatg caggccccat agcagcaagc gcgcgatcca cacgcaattg
    11941 atttatggca tccagtctct gtttcagagt ctcaatatag aggtacaaga cgtatcgcct
    12001 gatttgctac ccgtcatgac tgtgattccg ccaacatcaa cggtaacacg cggcattcgg
    12061 gatatttcgt atgtcaaagg taaccgttac cacttttcgc gcctggtttt tttagtttca
    12121 cgacgaaaaa atggtctaaa acgtgatcaa tttaacacct tgctgattga ccgtaaagaa
    12181 agatgcgcta catacaagtg tagcaccgtt tattctctgt aaattcctta ttacgacggc
    12241 gtgaaacgcc tgtcaggatc cactgccaga cctcatttta cggtttgcgc aggcgtctac
    12301 gtttcaccac aacactgaca tcactctggc aaggatgtta ggatggacca cggatgataa
    12361 tgacggtaac aagcatgtta gacaatgttt taagaattgc cacacgccaa agcccacttg
    12421 cactctggca ggcacactat gtcaaagaca agttgatggc gagccatccg ggcctggtcg
    12481 ttgaactggt accgatggtg acgcgcggcg atgtgattct tgatacgccg ctggcgaaag
    12541 tcggcggaaa aggcttattt gtaaaagagc tggaagtcgc gctcctcgaa aatcgcgccg
    12601 atatcgccgt acattcaatg aaagatgtgc cggttgaatt cccgcaaggt ctgggcctgg
    12661 tcactatttg tgagcgtgaa gatcctcgcg atgcctttgt gtccaataac tatgacagtc
    12721 tggatgcgtt accggcaggc agtatcgtcg ggacgtccag tttacgtcgc cagtgccaac
    12781 tggctgaacg ccgcccggat ctgattatcc gctccctgcg cggcaacgtc ggcactcgcc
    12841 tgagcaaact ggataacggc gaatacgatg ccatcattct tgccgtagcc ggactaaaac
    12901 gtttaggtct ggagtcccgc attcgcgccg cgttgccacc cgagatttct cttccggcgg
    12961 taggacaagg tgcagtcggt attgaatgcc gccttgatga tacacgcact cgcgagctgc
    13021 ttgccgcgct gaatcaccac gaaactgcac tgcgcgttac cgcagaacgc gccatgaata
    13081 cccgtctcga aggtggatgt caggtgccaa ttggtagcta cgccgagctt attgatggcg
    13141 aaatctggct gcgtgcgctg gtcggcgcgc cggacggttc gcagattatt cgcggtgaac
    13201 gccgcggtgc gccgcaagat gccgaacaaa tggggatttc gctggcagaa gagctactga
    13261 ataacggcgc gcgcgagatc ctcgctgaag tctataacgg agacgccccg gcatgagtat
    13321 ccttgtcacc cgcccgtctc ccgctggaga agagttagtg agccgtctgc gcacactggg
    13381 gcaggtggcc tggcattttc cgctgattga gttttctccg ggtcgacaat taccgcaact
    13441 tgctgatcaa ctggcagcgc tgggggagag cgatctgttg tttgccctct cgcaacacgc
    13501 ggttgctttt gcccaatcac agctacatca gcaagatcgt aaatggcccc gactacctaa
    13561 ttatttcgcc attggacgca ccaccgcact ggcactacat accgtaagcg gacagaagat
    13621 tctctacccg caggatcggg aaatcagcga agtcttgcta caattacctg aattacaaaa
    13681 tattgcgggc aaacgtgcgc tgatattacg tggcaatggc ggtcgtgagc taattgggga
    13741 taccctgacg gcgcgcggtg ctgaggtcac tttttgtgaa tgttatcaac gatgcgcaat
    13801 ccattacgat ggtgcagaag aagcgatgcg ctggcaatcc cgcgaggtga cgacggtcgt
    13861 tgttaccagc ggtgaaatgt tgcagcaact ctggtcgctg atcccacaat ggtatcgtga
    13921 gcactggtta ctacactgtc gactattggt cgtcagtgag cgtttggcga aactcgcccg
    13981 ggaactgggc tggcaagaca ttaaggtcgc cgataacgct gacaacgatg cgcttttacg
    14041 ggcattacaa taactctcat aacaggaagc cataatgacg gaacaagaaa aaacctccgc
    14101 cgtggttgaa gagaccaggg aggccgtgga caccacgtca caacctgtcg caacagaaaa
    14161 aaagagtaag aacaataccg cattgattct cagcgcggtg gctatcgcta ttgctctggc
    14221 ggcgggcgtc ggtttgtatg gctggggtaa acaacaggcc gtcaatcaga ccgccaccag
    14281 cgatgccctg gctaaccaac tgacggcatt gcaaaaagcc caggagagcc aaaaagtcga
    14341 gctggaaggc attattaagc aacaagctgc acagcttaag caggcgaatc gtcagcaaga
    14401 aacgctggca aaacagttgg atgaagtcca acaaaaggtc gccaccattt ccggcagcga
    14461 tgctaaaacc tggctgctgg ctcaggccga ttttctggtg aaactcgccg gacggaagct
    14521 gtggagcgat caggacgtca cgaccgctgc agcgttgctg aaaagtgcag acgccagcct
    14581 ggcggatatg aatgacccga gtctgattac cgttcgtcgg gcaattaccg atgatatcgc
    14641 cagcctttct gcagtatcgc aggtggatta tgacggcatc atccttaagc ttaatcagct
    14701 ttcaaatcag gtagataacc tgcgtctggc cgataatgac agcgatggtt cgccgatgga
    14761 ttccgacggt gaagagcttt ccagttccat cagcgaatgg cgtatcaatc tgcaaaaaag
    14821 ctggcagaac tttatggaca acttcattac gattcgccgt cgtgatgaca ccgccgtacc
    14881 gctgttagcg ccaaatcagg atatctatct gcgcgaaaat attcgctctc gcctgctggt
    14941 cgcagcacaa gctgtaccgc gtcaccagca agagacttat cgccaggcgc tggagaacgt
    15001 ctccacctgg gtacgtgctt actacgatac tgatgatgcc accaccaaag cgttcctcga
    15061 cgaggtagac cagttaagcc agcaaaatat ctcgatggat cttccggaaa ccctgcaaag
    15121 ccaggcgatg ctggaaaaac tgatgcagac tcgcgtgcgt aacctgctgg cacaaccggc
    15181 agcggggaca acggaagcta aacctgcacc tgcacctgca cctgcacctg cacctgcacc
    15241 tgcacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg caccgcaagc
    15301 tgatactccg gcagccgcgc cgcaaggaga ataaccatgc taaaagtgtt attgctcttt
    15361 gtgttgctga ttgcggggat cgtggttggc ccgatgattg ccggtcatca gggttatgtg
    15421 ctgatccaga ccgacaacta caatatcgaa accagcgtca cgggcctggc gatcatattg
    15481 attctggcga tggtagtgct gtttgccatt gagtggctac tgcggcggat cttccgcact
    15541 ggcgcgcaca cccgtgggtg gtttgtcggg cgtaagcgtc gccgtgcacg taagcagacc
    15601 gaacaggcgc tgctgaaact ggcggaaggc gattatcagc aagttgaaaa gctgatggcg
    15661 aaaaatgccg atcacgctga acaaccggtg gtgaactatc tactggctgc cgaagccgcg
    15721 caacaacgtg gtgatgaagc gcgcgccaac caacatctgg aacgcgcagc ggagctggcc
    15781 ggcaatgaca ccattccggt agaaatcacc cgtgtacgtc tgcaactggc ccgtaatgaa
    15841 aaccatgctg cacgccacgg cgtggataag ctgctggaag ttacgccacg ccatccggaa
    15901 gtattacgtc tggcggaaca ggcgtatatc cgcacaggtg catggagttc gctgctggat
    15961 attatcccat caatggcgaa agcccatgtt ggtgatgaag aacatcgtgc aatgctggaa
    16021 caacaggcat ggattggcct gatggatcag gcgcgtgccg ataacggtag cgaaggtttg
    16081 cgtaactggt ggaaaaacca aagccggaaa acgcgtcatc aggtagcgtt gcaggtggca
    16141 atggcggaac atcttattga atgtgacgat catgatactg cccagcaaat tatcatcgat
    16201 ggcctgaaac gccagtacga cgatcgccta ctgctgccga ttcctcgact gaaaacaaac
    16261 aatccggaac agctggaaaa agtgctgcgc cagcaaatca aaaacgtcgg cgatcgcccg
    16321 ctgttgtgga gcacactggg ccagtcactg atgaagcacg gagaatggca ggaagcatcg
    16381 ctcgccttcc gcgcagcgct gaaacaacgt ccggacgcct acgattacgc atggcttgcc
    16441 gacgcgctgg acagactgca caagccggaa gaagctgcag cgatgcgtcg cgacggtttg
    16501 atgttaacgt tgcagaacaa cccaccacag tagttccttc tcacccggag gcaagcacct
    16561 ccgggacctt cctgacacat aaaaaaacgc ctgctcttat tacggagcag gcgttaaaac
    16621 aggtctgtat gacaacaagt gggtgcttca ctcaacgttg tgtccatggt gtctgatgag
    16681 gcataagcga catctgtcag tggacgataa gcaccgtaaa cggctctgcg tcattccgga
    16741 gtttatgagg cactaaggcg aacataagag atggaatgag catctactcg tttattatgc
    16801 cacagagaat cgggaaataa catcccttaa cacttgttat gagataattc tgtaatcctc
    16861 tttgcttcct gagtaataac ttcctgagtg aatatttaac ctgagcttga tcctacacat
    16921 acttattatg aatgataaaa ttcattcaat taataacaca tatattaatt gccgttaaaa
    16981 ctaaaaacag catcaataat caacgcgata taataaacct gccttacata tcaactgcgc
    17041 cagaggtagg attgaaaacg ctctcctgat ttttaaattc atttcttgga taaataaata
    17101 atttattttt atcactatta tttatgtaat catcctgtca gggagaggga tctcaattat
    17161 caatgcttaa ttacgtcatc attttgttga catgaaaccg gagcattttg catggaattt
    17221 tcgttttcac ccaaacttct tgttgttgct gtcgccgccg ctcttcctct catggccagc
    17281 gcagcagata ccccgtcaac tgccaccgcg cgcaaaggct ttgccggata cgatcaccca
    17341 aaccagtatc tggttaaacc ggcgaccact attgccgaca acatgatgcc agtaatgcag
    17401 catccggcgc aggataaaga accccagcag aagctggcag aacttgagaa aaaaaccggt
    17461 aagaaaccga atgtggttgt tttcttgctg gacgatgtgg gctggatgga cgtcggtttt
    17521 aacggtggcg gcgtggcggt gggtaaccct acaccagata tcgacgccgt tgccagccag
    17581 gggctgattt taacttcggc gtattctcaa ccaagctctt ccccaacccg cgccaccatt
    17641 ctcaccggac aatactccat ccaccacggc attctgatgc cgccaatgta cgggcaaccg
    17701 ggcgggctgc aagggttaac cacgctgccg cagttgctgc acgatcaggg ctacgtcact
    17761 caggccatcg ggaaatggca tatgggggaa aacaaagagt cgcagccgca gaacgttggc
    17821 tttgatgatt tccgtggctt taactcggtg tctgatatgt acaccgaatg gcgcgacgtt
    17881 cacgtcaatc cggaagtggc cctgagtccg gaccgttctg aatacatcaa gcaattacca
    17941 tttagcaaag atgatgttca tgcggtgcgc ggcggcgaac aacaggccat tgccgacatt
    18001 acgccgaaat atatggaaga tctggatcaa cgctggatgg aatatggcgt taagttcctc
    18061 gacaagatgg cgaagagtga taagcctttc ttcctctatt acggcactcg cggctgtcac
    18121 ttcgataact acccgaacgc caaatatgcg ggtagctctc cggcacgcac ctcgtatggc
    18181 gactgcatgg tggaaatgaa cgatgtgttc gctaatctgt ataaagcact ggagaaaaac
    18241 ggtcagcttg ataacacgct gattgtcttt acctccgata acggaccgga agccgaagta
    18301 ccgccgcacg gacgcacacc gttccgtggt gcgaaaggat ctacctggga aggcggcgtt
    18361 cgcgtaccga ctttcgttta ctggaaaggc atgatccaac cgcgtaaatc tgacggtatt
    18421 gttgatctgg cagatctctt cccaacagca ctggatctgg cggggcatcc tggtgcgaaa
    18481 gtggcgaatt tagtgccgaa aaccaccttt atcgatggtg tggaccagac atctttcttc
    18541 ctgggaacaa atggtcagtc taaccgtaag gccgagcact acttcctcaa cggtaaactc
    18601 gctgctgtgc gtatggatga attcaagtat cacgtcctga ttcagcaacc ttacgcttat
    18661 acccagagcg gatatcaggg tggattcacc ggcacagtaa tgcaaacagc cggatcgtcg
    18721 gtgtttaacc tctacaccga tccgcaggag agcgactcta tcggcgtgcg ccatattccg
    18781 atgggtgtac cgctacagac cgaaatgcac gcgtatatgg agatcctgaa aaagtatcca
    18841 ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg ctgagatctg cctttgccgg
    18901 atgcgatgct gacgcatctt atccagccta cagaacgctg caatttattg aatttgcacg
    18961 atcatgtagg ccggataagg cgtttacgcc gcatccggca atcaaccgca ggcgaccaca
    19021 aaatggccgc ttttttactt actcaccacc agcaaatgcg cctgcataat gtcgctggcc
    19081 gggcgaccgt gcgccagcaa atcagccatt gctttaagat atggcggtag atggcggaaa
    19141 taacgctgat acccggcaca caaataattc agtcccggtt tgccgctggc atcgagcatg
    19201 aagcggtgtt tcgggcagcc tccccaacat gcttttaaca cgttacaact gcgacactgc
    19261 gccggtaatt gcttaaattt atcttcacca aacacctgct gttgcgggga atcgatcatt
    19321 tctgcaattg tttgctggtg catattcccc agtcgatatt gcggataaac atagtgatcg
    19381 caggcgtaaa cgtcgccatt gtgctcaaca atcaccgagc gcccacaggt tggctgatga
    19441 tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg cccattcgat attcatcacg
    19501 aaaatcttgc cgacgtcgcg tttaatccag tggtcgaata tcgccaccag aaattcacca
    19561 aattcatcgg ggcgcacaga ccattccgtc agttccccct gaatatcacc aggcgcatgt
    19621 agtttcagtc cttcgcgggc agtcgtttca tcagccaggc gctcgaccac cggaataaac
    19681 tggatgaatt cgactcccgc atcgcacaaa aaatcataaa cctgtaacgg ttgctgcgcg
    19741 ctggtgcggt tgacgcagac cagcacgtta tagtcgacat gatgtttttg caggagcgtc
    19801 agggcacgca tcaccagttt atgcgtgggt ctgccgcctt ttgtcacgcg atattgattg
    19861 tggatctcag ccggaccatc cagcgataac ccaacaagaa aatgattttc tgccagaaat
    19921 gcacaccatt catcatcgag cagtacgccg ttagtctgga agctgttact tatcttcctg
    19981 ccagcaccat atttcgcctg tagcgccacg gcacggcggt aaaactccag cccgagtagc
    20041 gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt gcggttcgct ggcagcgata
    20101 tagtgacgga catacgcttc cagcgtgtca tcgtccatat gcgtgactgg cttttcgcgg
    20161 taaagggatt gtttttcgag ataaaaacag tagtcacagt tcagattaca atcggaaccg
    20221 ctcggtttcg ccatcacatg aaaagcacgc gttggaacct gttgcagcat ggtcgctcct
    20281 tgcctgaagt catccttcaa tacgttaagg gcgaaatcat accgtggggc aatgagttat
    20341 tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca gaaaacaaaa aaccccgccg
    20401 aagcggggtt caaaattggt cggcgagaga ggattcgaac ctccgaccca ctggtcccaa
    20461 accagttgcg ctaccaagct gcgctactcg ccgaaatact gctttttgaa tttttagttc
    20521 aattctttaa agtcgtggtg cgaggggggg gacttgaacc cccacgtccg taaggacact
    20581 aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcattgtcac aacttctaat
    20641 aatggggtgg ctaatgggat tcgaacccac gacaactgga atcacaatcc agggctctac
    20701 caactgagct atagccacca ctacaaatct tgttacgcgg tattactacc accgcagctc
    20761 aagcgccggg actaaatggc gcgcccgaca ggattcgaac ctgagacctc tgcctccgga
    20821 gggcagcgct ctatccagct gagctacggg cgcttagcgc cgttgcgggg gtggataata
    20881 cggacttccc ccccctctgt ctagtccctt tttaaataaa ttgcgcgttt ggttacggtt
    20941 tgtgcgtttt gctgcttatt cctccagttt atgcgctttc ccgtggcgat taaggccaaa
    21001 aactttataa atcgccgtca ccgccagcat aaagatgatg ccaacaaaca gcgacatacg
    21061 cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca ttgttacgta
    21121 gttggcccac gggaacagga ttgagcggaa cggatggctg gcaatcgccg ctttatgtgc
    21181 acgccgaaaa cgcagctggc ttatcaatat cacaaaccat ggcaccatcc ccggaagcac
    21241 gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt agttcaggca
    21301 tgagccaatt agcagaatag caatagatac tgccacaccc gcaaccggta cgccgtgacg
    21361 ggaaactttc gccatcgccg ccggtaactg acggtttttc gccagtgcgt agagcatacg
    21421 tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca ccacaaagtt
    21481 gataatgccc gccgctgcgg taataccgat tttggcaaaa gtcagtacga acgggctgcc
    21541 gttgctgcct atttcattcc acgggaagat ggtgacgata acgaaaatcg cgccaacgta
    21601 gaaaatcagg atccgccaca gcaccttgcc tacggcactg cgcagcgtca cctgcggatt
    21661 cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg ccaccacaat
    21721 acacagcgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat gctctgtgag
    21781 attgctaaaa ccaatcgact gcccgccatt gccaaagcca aagaaaatca cgcccaggcc
    21841 aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga actcgatttc
    21901 gccgtacaac cgcaccgctg ccagattcgc caacgccacc agcgccactg cgatcaatgc
    21961 gggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa tggcggtgat
    22021 ttcagagatc cccaccgcca tccacataaa ccagtaagac caggcggtga gatagccaaa
    22081 gaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaaccg gttcgaggaa
    22141 caacatttcg cccattgaac gcatgatgaa aaagacgaac agcccggcga tgatataggc
    22201 caacaatacg gatggcccgg cccatttcag ggtactggcg gcccccataa acaggccgac
    22261 gccaatggtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc cacgctgtag
    22321 ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc cggtgttacc
    22381 ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg agaaatggca
    22441 aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg cgacggagtg
    22501 accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtaa tgccagcgta
    22561 aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc aggcggtaga
    22621 gccactccag ccccagcgtt tgccagattt tcggtgcgcg ttttacgtga ccggtgaaaa
    22681 catcgtaagt cccgccaacg cccatataca gcgcatctgg atgcaccaga cggcagtcgc
    22741 gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc gctccgctgg
    22801 catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca tcctgactgc
    22861 caacgatatt cacattccac tggttgcgca gtttagcttc agtttgcgcc agcacttcag
    22921 gtttaccgcc cacaagaaat accgtcgtcc cttctttgcc tgcgcgcgcc atcagctctt
    22981 cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt ttacgtactg
    23041 aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt aactccctga
    23101 cctcggcgtt ctcttcaatg gtcagcattt tttcagcatt aatggcaacc agcgttccct
    23161 gcttaagctg cccgtcagca aacagataat cgagggcatg ctgcatatca cgccaaccaa
    23221 tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc attgttatcc
    23281 ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg cgctttcaaa
    23341 aagccagtac aacagttttg cgatcatcag acatgcgccg aagaccacga taaaaaagac
    23401 cacgcgtgag acaaacgaat ccagcccttc acgcgccagc acgatcatat tgaagatcgc
    23461 cccaaagcag aaactgtgca atatcgcagc cttatagcga ttagtttcgc ggttgcccag
    23521 ctcatacagc cagtcgaacc atttgatgat cagtccaacc acaatcgccc cgagcgggat
    23581 gaacaacgcg ccgcccatca ccactagtga gcctataagc gtaggcgaga tcgccagtcc
    23641 ggagtggtta ttcagcactt cccaggtaaa gtagttggct gagttcagca ccatactcgg
    23701 gcgacccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa ttggcgccag
    23761 gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct cccacggcga
    23821 gaaggtgtcg cgagtgagat agagaaacgt atagaacgct tcatcgccgc tcacattcat
    23881 tccatagcgt tttagcgcca gccagaacat gccaacaatc cccagcacgc ccgccgccgc
    23941 cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga tagcgaatgc
    24001 gatgatgata ttggcgcgag tgccgccgac aatcatataa gtcagcaagc caaaggcgac
    24061 cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg cgcagaaagt agaccaccag
    24121 catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc acgccggaga cttcactgga
    24181 aaagatctga ctgtaggagt taagccggaa cagcaaaaag ccgttgtgca tgaagaagat
    24241 gccgacgctt accagcgcga tacccatcag gatcacccac gtaagattgg tttccacgcg
    24301 gttcatggta aacagcggac ggcgcggtac atcagcaacg cgtttgcgta ggcgggtttt
    24361 gtaggtgacg tagtaaaccg cgtagaagca gcccgcagaa agcaacgcct gcaacaggat
    24421 ttctggcggc gcgacaccaa catcaaagcg aaacaccagc acgctggtca gcgggaagcc
    24481 aaagaaaaag gtgagcagaa acagcaatga aaagaataca ttgaagttaa agcgcacacg
    24541 acgaaactca aaccaggtca gcgtggcaat aaacagcgtg cagagcagcc agacaacaaa
    24601 caggccactg aattgcagca gactcatgcg acctccccgg cggcaatcgc caacgcccgc
    24661 tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc caccgacgcc
    24721 aactggcgct gcgcttcacg cacaatatcc tcgttgagat cgtcggtagt aaacagcacc
    24781 ggtaaatgct gttccgtcat atcctgccag aacggatttt cccggttaag cacacaagga
    24841 atgcccgcct gaatcagtaa gcacagcgta ccaatgccct gctggcgggc aaaaataaag
    24901 taaccaagat cgcactgacg aagtagcgtc agataggcgt caaattccag tttttcactc
    24961 agaacttgca gattttcttc gctgaataac tccagccccg cctgacgaac ttcctcgatg
    25021 tacgcttcgt tatgaggcgg atatcccatc ggcaccacca cttttaccgt atcgccaaat
    25081 tgctgatgaa cggcgcgcaa ggcagcaata tgctcattgc tgcggtcgcc ggagttcccc
    25141 accagaatgg tcattttccc ttcacgttgc cgatcgttcg ccatcgtatt gagagaagca
    25201 tccatccgcg tcgggaagta cagcagttcg ccccgcacct ttgggtgcgt tttggcaaaa
    25261 aagctcaaat caccacgggt ggcaaataca cagccgactc gcttttgcgc caggcgacgt
    25321 agtgggtaaa aaagcttata tctcaagcca ctggaaagct cgtacaggtc tgccccccag
    25381 atatgccagt aaaactggct gggcttaatg ccaccactca gcagagccag ccacagtgtg
    25441 ggattgaact gaccgtggaa gaaaaaacgc tgctgacggt tagcttttgc tttcgcgatg
    25501 accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc cggacagcta
    25561 tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc gctcgtcgcg
    25621 gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat ggtgagggat atccgatccc
    25681 agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg ccacaacaaa
    25741 gagaaaaata gacgatatag gttgccatat atgcctgtgc cgcgcccagc gcgccgtgcg
    25801 tagggattag ccagtgggca aataccatca ataaagtgaa ctggctgact tccgccagaa
    25861 tataaaaccg cagtgaagct ttggcgatca ccagataacc aaagacataa gcgcccactt
    25921 ttaacacatc acccaccaac tgccaggcaa agagatcgcg catagcggta aatttattcg
    25981 acaacagcag ccagatagca aaatcacgca gcaaccagac ggtaaaactc gccgccgcca
    26041 ctgccggtaa gacgaatttc agcgatttaa ccacttcccg ggtgatatcg cgcttttccg
    26101 ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata aattgcaggt
    26161 aggcatcgga aatactgctc accccttgcc agatccccac ctcatcccag ctatactgcg
    26221 ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac gtaatcaacg
    26281 tcatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg ggtttcagat
    26341 agcttaacgg gatagcacca cgcttaatta acatgacggc ggcaggaatc accaccagcg
    26401 cgggaatcaa cgccagaccc agcaacgccc cttcatatcc gcccaaacgg taactgacgt
    26461 agtacgcaag aacgccaatc aagctgccaa caatcagaga taacgcatta cctgcggcat
    26521 cgcgaaagcc tttcatcaac gccagtaaca ggttgcccca ggcgatcccc atttgcacca
    26581 gcgccactaa acgcaccagc ccctgatagt cgtgattacc aaacaatccc aagctgattg
    26641 gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaagccaagt accatcgctg
    26701 atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc atggtactgg gcaacgtatt
    26761 tggttacacc gttaaagatg ccagccccgg caagcacgcc gagcacggta atcaactggc
    26821 ggaaatttgc cgccagccca agccccgccg gaccaaatga caccgccagc aacttaccga
    26881 ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc cgtccacaag gacgcttttg
    26941 ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa ttaccgtacg ctgattgacg
    27001 ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct cgctctcttt ggtggtgtag
    27061 cgatcttcac cgtggaactc accaaagcgt tcccccgcag ggcaaccgtg cagcggaatg
    27121 taatggaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc gctccggtca
    27181 ccaatatccc gcagtttaat gtaaaacata tgcgcgttct gcaagcagcc atcgggaatc
    27241 gacggcagct cgatacgccc ggctttcgcc agaggtgcta acgcatcgta gtagttttgc
    27301 cacagcgcca gacgttgctg gttgatacga tccgctgctt ccagttgcgc ccacaggtac
    27361 gcagcttgca gatcggacat caaatagctg gagccgatat cgcgccaggt atatttatcg
    27421 acctgaccac ggaagaactg gctgcggttt gtgccttttt cacggatgat ctcggctcgt
    27481 tcgatcaacg ctttatcgtt aatcagcgtc gcgccgcctt caccgcccgc cgtgtagttt
    27541 ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc acgccctttg
    27601 taagtggaca tcacgccctg agcagcatct tccaccacaa acagattatg ctttttcgcc
    27661 aacgccataa tggtgtccat ttcgcaggcc acgcccgcgt aatgcaccgg cacgataacg
    27721 cgcgttttgt cggtgatcgc cgcttcaatc aacgtttcgt cgatgttcat ggtgtccggg
    27781 cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa aggcattggc ggtggagaca
    27841 aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag cagcgccgcc
    27901 atctccagcg aagcggtgca ggacggcgtc agtaacactt tggcgctgcc aaaacgttgc
    27961 tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt gccgctaccc
    28021 attgccgact gcatatagtc gagttcggtt cccaccaccg gcggtgcgtt aaatggaatc
    28081 atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac tttgtatgta
    28141 tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc cgcaaagttg ttttaccgcg
    28201 agcatacgcc cagtttagcg ccgtttgcat cagctcagca cctgcaccgc gtccagccag
    28261 caggccaatt cgcgcatctg tagcattgag ttcccgtaaa gagacataac cgcgaatatc
    28321 gccggacgcc gcacgtaaaa tcagacattg atgatcaaag gtgccgcgca cggcattttc
    28381 aatccactgt gcataaaagc gactgctggc gtcaggcgca taccacggcg cacgaaaacg
    28441 gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg tctcttgtgc
    28501 cactacagca ccgctatcac tggcattgtt cacgggtagc gccaaatcaa cttcaccttc
    28561 taccagggag aatcccagct gttgcagggc atccagttcc cccgtatttg atgccgcaat
    28621 tttggcctgc acccgtgacc acggcgctaa cgcgtctggc gtcaggaacg gtgcttcaga
    28681 cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg ttttcccagg ttagtggctc
    28741 aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag ccagttttcg
    28801 ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag ccagccgtta cgccaggcga
    28861 tctcttccag gcaggcaatc ttaaagccct ggcgtttttc caccgtctgt acaaaggtgc
    28921 tggcttcaat caggctgtcg tgagtgccag tgtccagcca ggcaaatccg cgcccgagca
    28981 gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta atctccagtt
    29041 caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg tcgtagaaat
    29101 aaagcccggt caccgcccag tttgatttcg gctgttttgg cttttcttcc agcgagatag
    29161 cgcggaaatt atcgtcaaac tccaccacgc caaagcgttc cgggtccatc acctgatagc
    29221 caaaaaccgt cgccacttcc gtgcgcgccg caacatgacg cagcttcgga ctgaagccct
    29281 gaccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccatta aggaaggttt
    29341 caccgatgat aaaggcctgc gccagaccgt ccgggctggg ctgttcggca tattccagct
    29401 ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta tcttccggcg
    29461 tagtgatgat gagaatttcg cggataccgg ccagcatcag caccgacagc ggatagtaaa
    29521 tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta atcggatgca
    29581 atcgggtacc ggaaccgccc gccaggataa tacctttcat gtttgcctcc gctgaaaatt
    29641 agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc ttcctccagc
    29701 tttcattagc cagataccac tgcaccgttt tacgcattcc actttcaaag gtttcctgcg
    29761 gcagccagcc aagttcacgg gcaattttcg aagcatcaat ggcatagcgc agatcatgcc
    29821 ccggacggtc agcgacaaag gtgatcaagt cacgataatg caccacgcct tgcggcttgt
    29881 tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga ttcttacgct
    29941 cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc acgcaataca
    30001 gcgcgcgggc gtgatcttca acatacagcc agtcacggat ttgctgcccg ttgccatata
    30061 ccggtagcgg tttacccgcc agcgcgttga ggatcatcag cgggatcagt ttttccggaa
    30121 agtggtaagg accgtagtta ttcgagcagt tggtgataag cgttggcaga ccataggttc
    30181 gcagccaggc gcgcaccaga tggtcgctgc tggcttttga cgcggaataa gggctgctcg
    30241 gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca ccatatactt
    30301 cgtcagtgga gatatgatga aaacggaacg ctgatttttt atcttccgtc agcgcattcc
    30361 agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt tcaataaacg
    30421 ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc atgacacagt
    30481 ctggctgatg ctcagtgaat acgcgcgcca gttctgcccg atcgcagata tcaactttct
    30541 caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga catcaggttt ccggcgtagg
    30601 tcagcttatc gaccactacc accgcgtcgc tcgtttcgtt gatgatataa cgcaccagcg
    30661 ccgagccaat aaacccggca ccgcctgtta tcagaatctt tctcatcagc gccagactcc
    30721 tttagcatcg acgacatact gctgatggac attgtcgcca ttgataactt tgaactgact
    30781 atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa gctgcgccag
    30841 agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa caaccagagt
    30901 ttcgccgcta tgccactggg cgatcagttc agcgatttcc atcgccgggc tttcgcgcag
    30961 gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt cactggcgcg
    31021 tttatcggta gccgccaggc aatcagccac cgccgctttc acctgatcga taacccagaa
    31081 cggtttgtga tcgttcactt cgcgcgcggt acggataagc cgcgcctgct gggggttctg
    31141 tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag ggccaggctg
    31201 aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga cgttaatccc
    31261 ctgatcggca caaatcagcg acaattcatt agcaaaagcg atattcacat cgcggaagct
    31321 gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac actcaccttc
    31381 gaggaaaatt ttgtacagtt cgctggcgcg ggccgaacaa accggcgtca taccaccaat
    31441 cacgcgatcg tttttaatca gctcgaccat aacctggccc ggtaacacgc gttccgggca
    31501 gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt gggaaagtga gatccggacg
    31561 catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg tggattcaag
    31621 gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg attcaacgta
    31681 ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt acagcaatca gccaggcatc
    31741 cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag tttttactac
    31801 gctcgccaaa tcaggctcga cgatatggat ttcgccacga ttgatggtat caaccgcgtg
    31861 ttggttgata tcgacaccaa tcacctgttt ttgccgtgag gcaaacgctg ctgctgtagg
    31921 cagcccgatg taacccagtc cgataacaga aatggtcgca aaactcatag tgatatccga
    31981 ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata cgggttatgg
    32041 gcgcggctca tagtttgata ttcgttttcg tcttttaaaa gacgcgtcac ttcctcgaca
    32101 attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc ttccggacgc
    32161 tcagtggtat cgcgcatcac cagcaccggt ttccccagcg aaggcgcttc ttcctgaatg
    32221 ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa cggtaaatac
    32281 tcctgaggat cgatcagaat gacatttttc acatgcccca gaatgcgatt gaccggctct
    32341 ctgacgttcg ggttgagatg caccggatag acaatctgga tgtcctggtg cgtggtggcg
    32401 atgtctgcca gcgcgtggca gatttcttca aagccacgac cgaagctttc acgcctgtga
    32461 ccggtcacca gaatcatctt tttatcgggg tcgataaacg ggtaatttgc cgcaagttct
    32521 gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc ataacagtgc atcaatgact
    32581 gtattaccgg taatgaagat tcggctatct gcaacgtttt cacgcagcaa gttttgccgg
    32641 gaagtttcgg ttggagagaa atgatacatc gccagatgcc cggtcaatgt acggttagcc
    32701 tcttccggcc acggcgaata gagatcgccc gtgcgtaaac cagcctcaac gtgaccaaca
    32761 ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgtcgttgt gtcgccgtga
    32821 accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc cagaatccga
    32881 caggttatct ctgtcaggcc ctgtcctggc tgcattatgt tgagatcgta gtcaggtaca
    32941 atggaaaaga gtttcagcac ctgatcgagc atctcccgat gctgcgcagt gacgcaaact
    33001 ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggagccat cttgatggct
    33061 tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga ataagcggcg
    33121 agcgcctttg cgctcaccgc agcagtgttg ctatttcgag caacggcggg ttaatgcgac
    33181 accagccccg atcagccccc cgacaatgcc ccacataatc atcaggaagg cacgacgtgg
    33241 gctatcgcgt tttaccggtt cttccggcgt acgcaaatag cgataggtct gaaaacgcgg
    33301 atccagggtt ggaccaacat tcagggtgtt taacatggcc cgattctgat catagtcgag
    33361 atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca ttggacgccc
    33421 aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg cactgcgcga
    33481 aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg ctgttcatcc ggcggtcgta
    33541 gatggctttc gccacctctt cctgacgctt cacctgtgct ttcatctgga tggtacgcgc
    33601 cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc tggcaaaagc
    33661 aacatactga cgtaacaggt tattagcgtc aggcgcggtt tcggcaataa gcttcacgct
    33721 gtcattgacc gcgcgggtaa agtctccggg gataaactgg atgttgttaa tcatttcatc
    33781 cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgcttgtaat agtcggtttg
    33841 cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact ctttataggc
    33901 ttcgtccatg accgatggtt ggtcggcaga agccatgttt gaacggacat ccaggttacg
    33961 caaaaattgc tgctgcgagt aatatccccc cagcatattc accgttggac gatcggtaat
    34021 cgccgtcgag ctccactcct gacgagcaaa aaacgtatac gccagcgcga ttaacgcaaa
    34081 cgccagcccc atgccaataa tccatagctt cccagcccac aaggtacgaa acaacccacg
    34141 aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt gtgtcatcac
    34201 atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag tctgcgtttt
    34261 acgcgcttaa taaagcgagc aactttccag gcacgcttaa tgcaatatcc atagaggagg
    34321 aatgctagca aaaagagcac caacatgacc cactccggga caaaatgaga atattctgcc
    34381 agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa tcagcacaaa cgcctggcgg
    34441 gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtcagg agagaatggg
    34501 ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag cggaatggcg
    34561 attatccaca aagcggtaac cgggctgatg ggatgggttt tgccttgggt cgtttcgagc
    34621 aggatccaga tcacggtaaa accaatcagc gtactgcccg catcacccat aaagactttg
    34681 tagcggcgac ccaggatacc aaggttaagc atgatatatg gcaggatggc ggcgatcatc
    34741 gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc acaaaatcat accgattgct
    34801 gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa tgccatcaac catgttgaac
    34861 gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg tccgagcacc
    34921 atctcccagg agccaaagat ataacccagg ctactgagat aaaggttgcc aaacaccatc
    34981 ataacaatgc caacagcggc ctgtatggtg gcacggattt ttacgctgat atcaaaacgg
    35041 tcatccagcg cgccaatgaa aacaagcaca ccggcacaag cgagatagag agatgcatgc
    35101 ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac cgaaataccc
    35161 ccaacgagtg gtatcaaccc ctggtgacgt ttgcggaagt ttggtttatc cactaaaccg
    35221 actttttttg ccaccttacg ggcaaaaaac agaaacagtg tcgtgaataa aaaaatactg
    35281 atgagatcag tactcactgt cagtaaattc acaatgcatg ctctcagaga aaattattag
    35341 cagaagtata accacgaaga cctttattca gaagggaaag tccgttccga acccaccagt
    35401 cctgtgcaaa aattaaccat ttgatatgca aattgcataa catgccagca aattccagga
    35461 attttcctaa catcgccagc gcggcattaa gattacagat ataaaagcaa aacgccacgt
    35521 aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt tcgaagaaat
    35581 cgtcattggt cttggtcatt gccagtttat taatgaggaa ttccattgca tcgatttcgc
    35641 ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc tgagtcgtga
    35701 gcagctcttc tttacgggta ccggaacggt tgtagtcgat agccgggaag acgcgttttt
    35761 cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac tcttcgtaaa
    35821 taacttcgtc cattttagaa ccggtatcga taagcgccgt cgcgataatg gtcaggctgc
    35881 cgccctcttc cacgttacgc gccgcaccga agaagcgttt cggacgatgc agggcgttgg
    35941 catccacacc accggtcaac actttacctg acgccggaac aacggtgttg taagcgcgcg
    36001 ccagacgagt gatggagtcg agcagaatga taacgtcttt cttgtgctca accaggcgtt
    36061 tcgccttctc gatcaccatt tccgcaacct gaacgtggcg agatgcgggt tcgtcaaagg
    36121 tagaagcaac aacttcacct tttaccagtc gctgcatctc ggttacttct tccggacgtt
    36181 cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg ctctgagcaa
    36241 tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga ccacgctgac
    36301 cacgaccgat aggtgatgcc agatccagta cgcgagcggt taaatcttca gtagaaccgt
    36361 taccacgttc catacgcaga cgagagtttg cgtgcagcgg ggttaagttc tcaaagagga
    36421 ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc agcagcgcaa
    36481 aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca ccagtgcgga
    36541 ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg gcgaggtagg
    36601 agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt acgccatcac
    36661 caaagatatc ttcgccactc tttgcgtgct gcttcaggat agcaaaaata atgtcctgct
    36721 tacgcatacg agccaggttt tccagcccca tattttcgcc gagagtgatc agctcagaaa
    36781 ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa cttggggtaa
    36841 atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt tacggtcatc
    36901 gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa cgacaagacg
    36961 gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg ggaagcgttg
    37021 gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca ctaagttagc
    37081 atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga aattccctta
    37141 cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc ccactttggt
    37201 tgccgccact tcaccgtttt tgaacagcag cagagtcggg ataccacgga tgccatattt
    37261 cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt tgccctgata
    37321 ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc accactctgc
    37381 ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa aactgtcgtc
    37441 agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc tggcgtgttg
    37501 gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac cggatacgct
    37561 ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac atttaacaga
    37621 acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg aaaaaaaagg
    37681 gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg cgggtcgtga
    37741 cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta cgtcaacgtt
    37801 tcattgtctt ctctctcatc ccgcgattgc cgatcgcaag gtgaatcagc cgcgtgcctt
    37861 aattatggca ccgacgcgtg aacttgccgt gcagatccat gccgatgcgg aaccgctggc
    37921 ggaagctacc ggcctgaagc tgggtctggc ttacggtggt gatggttacg acaaacagct
    37981 gaaagtgctg gaaagcggcg ttgacattct gattggcacc acggggcgtt taattgacta
    38041 cgccaagcag aaccacatta acctcggtgc cattcaggta gtggtactgg acgaagccga
    38101 tcgcatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc gtatgccgcc
    38161 tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt tcgtaccggg tacgtgaact
    38221 ggcgttcgag cagatgaaca atgccgaata tattgaagtg gaaccggaac agaaaacggg
    38281 ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc gtttgctgca
    38341 aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaata ccaaacaccg
    38401 ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt tgttgacagg
    38461 cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg gcgatctgga
    38521 tattctggtt gccaccgacg ttgccgcgcg tggtttgcat attccggcag tgacgcacgt
    38581 ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg gtcgtacagg
    38641 tcgcgcaggc gcaagcggtc actctattag cctggcgtgt gaagagtatg cattgaattt
    38701 gcctgctatt gagacctata ttggtcactc aattccggta agcaaataca atccggacgc
    38761 attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccgcgcacag gcaatggtcc
    38821 gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt taaataaaaa atgtcatact
    38881 caaaatcatt caaactgtat caagatggca agagaatgaa tcccgatgag cttactgtag
    38941 taagtgattc aggtgaaaga atgcagccaa cacagagaca gattgaagga tgaagagtat
    39001 gggttccacc tcatcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct
    39061 ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg cgaataaaac gcaaagtgcg
    39121 tctggctgct ggcctgaata gcgaaaatgc cctctccaat gaagcaatgg agcgcggttg
    39181 gcaatgtttg cgcctgtttg ctgaacgtct gcaagatatt cctccctcgc aaattcgcgt
    39241 tgtcgctacg gcgacgttac gccttgccgt caatgcgggt gattttattg ccaaagcgca
    39301 ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa gaggaagcac gtctgattta
    39361 tcagggtgtt gctcacacca ctggcggtgc cgatcagcgc ctggtagtgg atatcggcgg
    39421 tgccagtact gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc
    39481 gatgggctgc gtcacctggc tggaacgcta ttttgccgat cgtaatctgg ggcaggaaaa
    39541 ttttgatgct gcagaaaaag cggcacgcga agtgttacgt ccggttgccg atgaattacg
    39601 gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt tacaggaaat
    39661 catgatggca caggggatgg atgaacgcat taccctggaa aagttgcagc aattgaaaca
    39721 gcgagccatt cattgcggtc ggctggagga actggagatt gacgggctga cgctggaacg
    39781 tgcgttagtg ttcccgagtg gtctggcgat cctgatcgcc atttttaccg aactgaatat
    39841 tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gcatgttgca
    39901 tctggccgtc gagcaggata ttcgcagccg tacgctgcgt aatattcaac gccgctttat
    39961 gatcgatatt gatcaggcac agcgcgtagc caaagttgcg gctaacttct tcgatcaggt
    40021 ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct
    40081 tcatgaaatc ggcctgagcg ttgacttcaa acaagcgccg caacacgctg cttatctggt
    40141 gcgtaatctg gatcttcccg gttttacccc cgcacagaaa aaattgctgg cgacgctact
    40201 gctcaaccag actaatccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccgcc
    40261 gcgcgtcgca gaacaactct gccgtttact acgcctggcc atcatttttg ccagccgtcg
    40321 ccgtgacgat ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac
    40381 gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa gagattattg ctcaggaaag
    40441 ccagtggcag agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg
    40501 cgatgctgac gcatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct
    40561 ttcagattcg ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg
    40621 cgctcctgtt cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg gctcggctcc
    40681 gggcgcacca gttcgccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc
    40741 tgggcgcggg taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg
    40801 atactgctct ggtgcggcaa aaacccttct tccataccga ccatgtagac ataaggaaac
    40861 tccagtcctt ttgacgcgtg gagagtcatc agttgcacct gatccagctc ttcttcactc
    40921 tcaccacgct ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc
    40981 ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg
    41041 ttcttcatgc gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca
    41101 taatccatgc catggatcag atcacgcacc gcagcaatcg gctcccgctc cgccaggcgc
    41161 tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg tccgctaagc
    41221 gtctgactca ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc
    41281 cactcaccca gctttttcag cgtagccggg ccaatctctc gcttcggcgt gttaacgata
    41341 cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc
    41401 ttgatttcag gacgagagaa aaacgacgta ccaccggata ttttgtacgg gatgcggttt
    41461 tgcatcagga atttttcaaa tacccgcgac tgatggttgc cgcgataaag aatggcgtaa
    41521 tctttgtact gcgttttatt gacgaagtga tgggcgatca gctcgccagt aacgcgctcg
    41581 gcttcatgtt cctcgttatt cgcgcttaaa acttttagct ctgtgccgta acccagttcg
    41641 gagaacagac gcttttcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc
    41701 agaatacgcc cggaagagcg atagttctgc tcaagcttaa tcaccttcag cgccggaaaa
    41761 tcctgactca gcagcaccag gttttgcgga cgtgcaccgc gccaggagta gatcgactgg
    41821 tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagttca
    41881 tactggctgg tgttggtgtc ctgatactca tccaccagca ggtagcgaat tttgttctgc
    41941 cagcgttcgc gtacttcttc attgcgttgc agcagcaacg tcggcaataa aatcaggtca
    42001 tcgaagtcga gaacgttaca ggctttcagg tgtgcatcat acagcccata acaatgggca
    42061 aaaatacgat cccgctcgcc ctttgcctct gccgccgcct gcgccggagt tttgagatcg
    42121 ttcttccagt tagagatggt cgaaatcagt tgttgcagga gaactttgtc atcttcaatc
    42181 agcccctcgg tcaactcttt aagcaaagca agctgatcgg tatcgtcaaa taacgagaag
    42241 ttcgctttca tcccaagcgc cgcatactcg cgtttgatga tatccagccc caacgtatgg
    42301 aaagtggaga tcatcagccc acgcgcctct ttacgcccca gcgtctgccc tacacgctct
    42361 ttcatctcgc gcgctgcttt attagtaaag gtcaccgccg caatgtgccg cgcctgataa
    42421 ccgcaaccgc ggatcagatg ggcgatttta ttggtgataa cacgagtttt accggaaccc
    42481 gcgcccgcca gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg
    42541 tttagacgca taggtgtatt gctcaatctt cgaacggggg gaggattgta gcagaatcgc
    42601 atttgaatgt gaccgcgtgt aagccgctaa ccggttggct gcggatgttt aaatcagaga
    42661 gagaagaccg aaatcggagt tcatcgtgaa tccattagac ttagaaaata tcgggtcgta
    42721 acgttgtgtt attacttttt cagagtttac taaggagagg tttactcgtg gcgattaatt
    42781 tcagccctaa ggttggtgaa atactggaat gcaactttgg taactatcct gttagccaaa
    42841 atggaccgtt cagcacgacg tattacgatg gccgcattcc tcctgaaatg ataaaaaaca
    42901 ggttagttgt cgtgctcaac ggaaaaatta atggcaacgc attcatcgtc gttccattat
    42961 caaccactcg cgaccatgac aagttaaaac gtggaatgca tgttgaaata gccagtaatg
    43021 ttatcaatga cctgcaattc tttgatcagc agattcgttg ggccaaaacc gacctggtac
    43081 aacaagtcag tcgaaacaga ttaaacagag caagaactta cagagggtat cttaatcaat
    43141 gtttgccaca tgagttggtt gcagacatac agagggcggt catcaagtca atcaatgcca
    43201 tatcgctcat aaattgacaa taaaatcacc atccccgata atgactttgt agcccataga
    43261 ggccctaagt tttctcccct tgagggacct gcgaagcccg gattttccgg gcttttgcca
    43321 tatatgtacc agcccctccc tgactgacaa ttacgttagc atcggcggat catgctaaaa
    43381 tcgccgcctg acaattattc actcaaggca cgatcatggc aaaaacagca gcagcactgc
    43441 atatccttgt aaaagaagag aaactggctc tggatcttct cgagcagatt aagaacgggg
    43501 ccgattttgg caagctggcg aagaaacact ccatttgccc atcaggcaaa cgcggcggtg
    43561 atttaggtga attccgccag ggtcagatgg ttccggcgtt cgataaagtt gtgttctctt
    43621 gtccggtact ggagccaacc ggcccgctgc acacccagtt cggttaccac atcattaagg
    43681 tgctgtaccg taactaatag caaggccttc tccgggagaa ggccttgaac tctgaatcgc
    43741 tattaacccg caacagcaat acgtttcata tcagtcatgt agccgcgcag tttcttacct
    43801 acctgctcaa tcgcatggct gcgaatcgct tcgttcaaat cacgcagttg cgcgttatct
    43861 accgcacctt ccggaatagc tttacccaaa tcgcccggtt gcagctctgc cataaacggt
    43921 ttcagcaacg gcacacaagc gtaagagaac agatagttac cgtactcagc ggtatcagag
    43981 ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat cagcggcagt
    44041 tcgtgcagtg attcatagta agcagattct tcgatgatgc cggaatcaac catggtttca
    44101 aacgccagct caacgcccgc tttcaccatc gcgatcatca gtacgccttt atcgaagtac
    44161 tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt caaacgcggt tttgccggtc
    44221 tcttcacgcc aggtcagcag tttcttatca tcgttggccc agtccgccat catgccggag
    44281 gagaattcgc cggagatgat gtcgtccata tgtttctgga acagcggtgc catgatctct
    44341 ttcagctgtt ctgacaacgc gtaagcgcgc agtttcgccg ggttagagag acggtccatc
    44401 atcagggtga tgccgccctg tttcagcgct tcggtgatgg tttcccagcc gaactgaatc
    44461 agtttttctg cgtatgccgg atcggtgcct tcttccacca gcttgtcgaa gcacagcaga
    44521 gagccggcct gcaacatacc gcacagaatg gtttgctcgc ccatcaggtc agatttcact
    44581 tccgcaacga aagaggattc cagcacgccc gcacgatgac caccggttgc cgccgcccat
    44641 gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt ccgggtgaac ggcaatcagc
    44701 gtcggtacgc cgaatccacg tttgtactct tcacgtactt cggtgcctgg gcatttcggc
    44761 gcaaccatta ctacggtgat gtctttacgg atctgctcgc ccacttcgac gatgttgaaa
    44821 ccatgcgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt gtgcactaca
    44881 tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgtgg gatcagttct
    44941 tcgtaagtac ccactttaaa accattttcg gtcgctttac gccaggacgc gcgcttctcg
    45001 gcaatcgctt ctttacgcag agcgtaggag atatcgagac cagaatcacg catgttcagg
    45061 ccctggttca gaccctgtgc gccacagccg acgatgacca cttttttacc ctgaaggtag
    45121 ctcgcgccat cggcgaattc gtcgcggccc ataaagcgac atttgcccaa ctgtgccagt
    45181 tgctggcgca ggttcagtgt attgaagtag ttagccatgg tgattcctcg tgatgttgtg
    45241 cttcttattg ttcggttcgc gtttgcaaga ttgaattcac tatatgacag gaaatttatt
    45301 gcggaaattg atatattcac aacatcatat tgcaattttt gcaacgtcaa catcgagggc
    45361 tgtccctgtg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa gccgccattt
    45421 tggccgcagc gcgcgggcga tgcacgttag cccatccacg ctctcacggc agattcagcg
    45481 cctggaagaa gatctcggtc agccgctgtt tgtgcgcgat aaccgcacgg tgacgctgac
    45541 tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt atcagcagct
    45601 gcgccacacc atcgatcagc aagggccgtc gctctctggc gaattacata tcttctgctc
    45661 ggtgaccgct gcctacagcc atctgccgcc gattctggat cgcttccgcg cggaacaccc
    45721 gtcggtggag attaaactca ctactggtga tgcggcagat gcaatggaaa aagtggtcac
    45781 gggagaagcg gatctggcga ttgccgggaa accggagacg ctacccggcg ctgtagcgtt
    45841 ttcgatgctg gagaatctgg cggtagtgct gattgcgccc gcgctgccct gcccggtgcg
    45901 taatcaggtc tcggtagaga agccggactg gtcaacggta ccgtttatca tggccgatca
    45961 ggggccagta cgccgccgca ttgaactgtg gtttcgacgc aataaaatca gcaacccaat
    46021 gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc tcggctgtgg
    46081 tgtggcgctg ttgccggaag tggtgctgga aaacagcccg gagcctgtac gcaaccgggt
    46141 aatgatttta gatcgcagcg atgagaaaac gccgtttgaa ctgggcgtgt gtgcacaaaa
    46201 aaagcggctg catgagccgc tgattgaggc gttctggagg attttgccga accacaaatg
    46261 acgttgtcgc gcgagtaggc ctgataagcg aagcgctatc aggcattttt ccctaacccg
    46321 ccaaaaagaa cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtag cccagctcat
    46381 tcagccgggt ttcgaaatcc ggttcatggt cgccaagttc gaacgccgcc agtacgcgcc
    46441 cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc gtacccagcg
    46501 tgttgaggaa gcgcagcagc gcgcccggtg attccgggaa ttcgaagctg tagaggcgtt
    46561 cctgcaacgg atgtgacgga cgcccgccga ccatatagcg cacatgcaac ttcgccattt
    46621 cgtcgtcgga gagatccacc acgctgtagc cgccgtcgtt gagcatctgc aaaatttctt
    46681 tgcgctcttc gaggccgcgg ctcaggcgca cgccgacaaa gatgcaggcg tttttggcat
    46741 cggcaaaacg gtagttgaac tcggtgaccg atcgcccgcc aagcagttgg cagaatttga
    46801 ggaagctgcc tttttcttcc ggaatggtca ccgccaacaa cgcttcacgc tgttcgccca
    46861 gttcgcagcg ttccgagacg tagcgcaggc cgtggaagtt cacgttggca ccggaaagaa
    46921 tatgcgccag ccgttcgccg cgaatgttgt gctgggcgat atattttttc attcccgcca
    46981 gcgccagcgc gccagagggt tccgccaccg cgcgcacatc ttcgaataaa tccttcatcg
    47041 ccgcacagat cgcatcacta tcgacggtga tgatatcgtc gagatactcc tggcacaaac
    47101 ggaaggtttc gtcgccgatg cgttttaccg caacgccttc agcaaacagc cccacgcgcg
    47161 gcagatcaac cggatgaccc gcatccagcg ctgctttcag gcaggcggag tcttccgctt
    47221 ctacggcgat tactttgatt tgcggcatca gttgtttgat cagcaccgcc acgccagcag
    47281 ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc tgctggagca
    47341 gttccagcgc cagcgtgcct tgcccggcaa tcaccatcgg atggtcgaac ggcggcaccc
    47401 aggtgaaccc ctgctgctgt gacagttcga tcgctttggc tttcgcttca tcaaagttcg
    47461 cgccgtggag cagcacttcg ccgccgaagc cgcgcaccgc gtcgactttg atgtcggcgg
    47521 tggcggttgg cataacgatc agggccttca cgcctaaccg cgcagaagaa aacgcgacgc
    47581 cctgcgcgtg gttacccgca gaagcagtga tcacgccgtg cgctttttgt tcttccgtca
    47641 ggcccgccat catggcgtat gcgccgcgca gcttaaagct gtgcactggc tggcgatctt
    47701 cgcgcttcac cagaatcacg ttatcaagac gagacgagag cttttccatt ttttgtagcg
    47761 gcgtgacctg cgccgcttcg tagaccggcg cgcgcagcac cgctcttaaa tattcagcac
    47821 cttccggggc accggacagg ggttgcgagt cagccattat taacccccca gtttcgattt
    47881 atcgcgcacc gcgcctttgt cggcgctggt tgccaggctg gcataagcac gcagggcaaa
    47941 ggagatctga cgttcacgat ttttcggcgt ccaggctttg tcaccccgag cttcctgcgc
    48001 ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac ggttcggaat
    48061 gtcgatggcg atcaggtcgc cgtcttcaat caggccaatg ctgccgccgc ttgccgcttc
    48121 cggtgagacg tggccgatag aaaggccaga ggtgccgcca gagaaacgac cgtcggtgat
    48181 cagcgcacag gctttaccga gccccattga tttcaggaag ctggttgggt agagcatttc
    48241 ctgcatcccc ggaccacctt ttgggccttc atagcgaatt actaccacat ctccggcgac
    48301 aactttaccg ccgagaatcg cttctaccgc gtcgtcctgg ctttcgtaca ctttcgccgg
    48361 gccggtgaat ttgaggatgc tgtcatcgac gcccgcagtt ttaacgatgc agccgttttc
    48421 cgcgaaatta ccgtagagca ccgccaggcc gccgtctttg ctataggcgt gttccagcga
    48481 gcggatacag ccattggcgc gatcgtcgtc cagcgtatcc caacggcaat cttgcgagaa
    48541 tgcctgggtg gtacggatac ccgcagggcc ggcgcggaac atatttttta ccgcgtcatc
    48601 ctgggtcagc ataacgtcgt attgttccag cgtttgcggc aacgtcaggc caagtacgtt
    48661 tttcacatca cggttcagta accccgcacg atccagctcg ccgagaatac cgataacacc
    48721 accagcacgg tgaacatctt ccatatggta tttttgggtg ctcggcgcga ctttgcataa
    48781 ctgcggaacc ttgcgggaaa gcttatcgat atcactcatg gtgaagtcga tttccgcttc
    48841 ctgcgccgcc gccagcaggt gaagtacggt gttagtcgat ccacccatcg cgatatccag
    48901 cgtcatggca ttttcaaacg ccgctttgct agcaatattg cggggcagtg cactttcgtc
    48961 gttttgctcg tagtaacgtt tggtcaattc aacaatgcgt ttaccagcat tgaggaacag
    49021 ctgcttacgg tcagagtggg ttgccagcag cgagccgttg cccggctgcg acagacccag
    49081 cgcttcggtc aggcagttca ttgagttagc ggtaaacatc ccggagcagg aaccgcaggt
    49141 tgggcatgcg gaacgttcaa cctgatcgct ctgggagtca gatactttcg ggtctgcgcc
    49201 ctggatcatc gcatcaacca gatcgagctt gatgatctga tcggacagtt tggttttccc
    49261 ggcctccatc gggccgccgg aaacaaagat caccggaata ttcagacgca gggaagccat
    49321 cagcatcccc ggggtgattt tgtcacagtt agagatgcag accatggcgt cggcgcagtg
    49381 ggcgttgacc atatactcaa cggaatcagc gatcagttcg cgagatggca gtgaataaag
    49441 catccccccg tggcccatgg caatcccatc atccaccgca atggtgttga actctttggc
    49501 aacgccgcca gccgcttcaa tttgttcggc gaccagttta ccgagatcgc gcagatggac
    49561 gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct taccgaaatc
    49621 ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat tacgaccatg
    49681 agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt ctgtctcgta
    49741 aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga tctaaccagc
    49801 cccatttatc ttcggtttcg ccggtaaaga ggccgaagaa ggcttgctga atgcgtttgg
    49861 tgaccgggcc acaacggcct tcgccaacct gaataccgtc tacactgcgc actggcgtga
    49921 tttctgccgc cgtaccggac ataaagactt catccgccag gtacaaggat tcgcgcgaca
    49981 gcacctgctc acgcacttca attcccagct ctttcgccag tttgatgatg gcatcacggg
    50041 taatacccgg cagcgcggag gaggtgaacg gtggggtgaa cagcacacca tctttcactt
    50101 caaacaggtt ttcgcctgcg ccttcagaga tataaccgtt cacatccagc gcgatacctt
    50161 cctgataacc gtggcggcgc gcttcgctac ccaccagcag ggaagagaga tagttaccac
    50221 cggcttttgc cgcagttggg atggtgtttg gtgctgcgcg gttccaggag gaaaccatcg
    50281 catcgatccc ctgctccagc gcttctgcgc ccagatacgc tccccacggg aaagcggcga
    50341 taatcacatc ggttgagtat cccgctggcg ggtttacgcc catgccaaca tcaccaacga
    50401 agatcagcgg acggatatag gcgctggtga gattgttttt gcggatcacg tcacggcaag
    50461 cttccatcag ctcatcaatg ctctgcgaaa ccgggaagcg atagattttg gcggagtcat
    50521 gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgc gagtcgtagc
    50581 aacggatgcc ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc acatgcacct
    50641 tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct ttcttcgtgg
    50701 tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt tgtgctctgg
    50761 cagatggcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag taagtcgacc
    50821 gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct ggcggcggcc
    50881 atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac acgttctaag
    50941 gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat aatttctcca
    51001 acatttctga attactggca ccaggcggca ccagcggcca gacgttctca agttcgtcga
    51061 ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt gccgcttcaa
    51121 cctggtcttt acgggtgatg tgttggccag ggatgccgaa ggcgctggct aacttgagga
    51181 aatcggggtt atcggtaagg gtggtttcgc tgtatcgttc ctggaaaaac agttgctgcc
    51241 attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac ggtaactgct
    51301 tgcgttttac ggtgcccagc tcttgtacat tcatcatgaa agagccgtca ccggagatac
    51361 agacgacggt atcgtttggt cgcgcgactt gcgcgccaac cgccgccggt aaaccaaaac
    51421 ccatggtgcc taagccgctg gaggtaatga aattttccgg gcgagtgtgt gcgatgtgct
    51481 gcgcggccca catctgatgc tgccccacat ctgtggtcac aacgcaatcc gcaggtttac
    51541 gatccgacag ttgttttaac aacaacggcg cgtagatagc gtcaccggga tggtcgtaac
    51601 gccaggcatg ttcatcacgc agctgcgcgc agtattgctg ccagtcattg atatttaatg
    51661 gctgctgtaa tgctggtaac agcgcattta aatcaccctg taatgccaca tgtgcctgac
    51721 gcagcttgtt catttctgcc gggtcgatat ccatatggat aacactggcg tgtggtgcga
    51781 aggtgttcaa tttgccggtc acccggtcat caaaacgtgc acccacggcg atcagcaagt
    51841 cgcactcctg caccgcgaag ttcgccgctt tggtgccatg cattcccagc atgcccagat
    51901 agtacggata atctgcttca actgcgccca gccctttcag cgtgcaggtg gcaggcattt
    51961 ttgtggtagc gagaaattct cgtaaagcag gaactgcctg cgccataccc acgccaccac
    52021 caacgtacag catcggtttt tgcgcttttg ccagcatctg gcgcgcttgc tcaacttcgg
    52081 catgtgggaa agtcacttcg ttttcaacgg tggtgaacca cggttccagg tcgccgctgg
    52141 ctaactggat atcttttggg atatcgacca gaaccggacc aggacgacct gagctggcaa
    52201 cgtcgaatgc ttcagccata atgcgcggca actcttccag cgactgcacc agaaagctgt
    52261 gcttggtaca ggctaacgac aatcccagga catccacttc ctgaaatgcg tcagtaccga
    52321 taaacggtgc ggacacttga ccggtgatgg caacaacagg gatagaatct aacagtgcgt
    52381 ccgcaagccc ggttatcagg ttggttgcgc ccggaccaga cgtggcgata catacgccag
    52441 ttttgccggt agcacgggca taaccgatag ccgccattgc cgcaccctgc tcatgtcggc
    52501 acagcaagtg ctccacgccg ccgtcataca atgcatcgta aaccggcata attgcgccac
    52561 ccggataacc gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc acccactgtg
    52621 cgccattcat agttagttcc ccgtcctgaa tcttgggaaa cagaattttg tgctgttatt
    52681 cattgtctgc tcctcggtta tgtttttaag gtcaaaaaaa acccccggac ctttcggtgc
    52741 gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag tgcagccccg
    52801 cacggtggga taataatcac caccacgcta atcacgacca ggctaatcac tcgtagaagg
    52861 gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag ttaccataga
    52921 ttttgcgaat agcacaagat attttttggc cgttttttat gatggcttta ataatttcaa
    52981 tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaaattcaat ttcagtacga
    53041 atgttgttcc ctccctcgta gttcatcctt tgtccgctgc atttttgcga gcatcattcc
    53101 accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca ctaaaattca
    53161 cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca aggagggctt
    53221 atgtcactgt caattgttca tacccgcgca gccctgggag taaatgcgcc cccaatcact
    53281 gttgaggtac atatcagtaa aggtttgccc ggcttaacga tggtgggctt accagaaaca
    53341 acggtaaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat
    53401 ccggcgaaaa aaatcaccat taaccttgct ccagccgatc taccaaaaga agggggacga
    53461 tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac agccaataag
    53521 ttagatgaat atgaattagt cggagaactg gcgcttacag gcgctctgcg tggcgttccc
    53581 ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa
    53641 gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctggtagc cgatcatctg
    53701 caagctgtct gtgcgtttct ggaaggtaag cacgctctcg aacgcccgaa accaactgat
    53761 gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga gcaaggaaag
    53821 cgaggactgg aaattaccgc cgctggcggg cacaaccttt tactgattgg gccgccggga
    53881 acaggtaaaa caatgctcgc cagccgtatt aatggtcttt tgccagattt aagcaatgaa
    53941 gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa
    54001 tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaacggc gatggtgggc
    54061 ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt gctttttctt
    54121 gatgagctac ctgaatttga acggcgtaca ctggatgctt tgcgagagcc gattgaatcc
    54181 gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg tttccagctt
    54241 gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa ccgctgcacg
    54301 ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga ccgcttcgat
    54361 ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt agtgccggga
    54421 gaaagcagca ccaccgttaa acaacgtgta atggctgcca gagagcgcca atttaagcgg
    54481 cagaataagt tgaacgcctg gctggatagt ccggaaatac gcaaattctg caagcttgag
    54541 agcgaagatg cgcagtggct ggaagaaacg ctgatccatc tggggttatc gattcgtgcc
    54601 tggcagcggt tattgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc
    54661 acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat
    54721 ctgcaaaaac tactgacata aaaaagggca tttcgccctt tttattaatc gtcagaatcg
    54781 gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt
    54841 ggacgcttga tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggctca
    54901 cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg
    54961 ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg
    55021 gtgaaatcac catggcgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta
    55081 gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg
    55141 gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca
    55201 aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc
    55261 cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccagtc agcagtgagc
    55321 tttcgaatca gacaactgga aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac
    55381 aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc
    55441 acctggcagg ccgcccgtaa ggaggtggcg catacctcac gacataacga gttttctatc
    55501 ggtgccagcg cctcattgtg ggaatgtatg cttaatcagt ggctgggacg tttgtatcaa
    55561 aatcaggatg cccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg
    55621 gtaaaacagc tgcatgaacg ccagcttgat cttcttatta ccactgaagc gcccaaaatg
    55681 gacgaattta gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct
    55741 tctaaactaa agggagatct taactatctg cgactggagt gggggccaga ttttcaacag
    55801 catgaggcag gtttgatcgg tgctgacgaa gtgcccattc tgacaaccag ttctgctgaa
    55861 ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt cagctgggcg
    55921 cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat
    55981 gccatatggc tgcaaaatag cgataaaaat acgttgattc gcgatctatt gaaaattaac
    56041 gtgctggatg aagtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact
    56101 tcagagaggg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
    56161 aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg
    56221 aactacgccc ctaattag
//
LOCUS       DBFIFS010000008        48853 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.008, whole genome shotgun sequence.
ACCESSION   DBFIFS010000008 DBFIFS010000000
VERSION     DBFIFS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.008"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            43..605
                     /gene="srlA"
                     /locus_tag="N7A07_000471"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             43..605
                     /gene="srlA"
                     /locus_tag="N7A07_000471"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            602..1563
                     /gene="srlE"
                     /locus_tag="N7A07_000472"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             602..1563
                     /gene="srlE"
                     /locus_tag="N7A07_000472"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            1574..1945
                     /gene="srlB"
                     /locus_tag="N7A07_000473"
                     /gene_synonym="gutB"
     CDS             1574..1945
                     /gene="srlB"
                     /locus_tag="N7A07_000473"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU2475207.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1949..2728
                     /gene="srlD"
                     /locus_tag="N7A07_000474"
     CDS             1949..2728
                     /gene="srlD"
                     /locus_tag="N7A07_000474"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU2475208.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2834..3193
                     /gene="gutM"
                     /locus_tag="N7A07_000475"
     CDS             2834..3193
                     /gene="gutM"
                     /locus_tag="N7A07_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU2475209.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3260..4033
                     /gene="srlR"
                     /locus_tag="N7A07_000476"
                     /gene_synonym="gutR"
     CDS             3260..4033
                     /gene="srlR"
                     /locus_tag="N7A07_000476"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU2475210.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            4026..4991
                     /gene="gutQ"
                     /locus_tag="N7A07_000477"
     CDS             4026..4991
                     /gene="gutQ"
                     /locus_tag="N7A07_000477"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU2475211.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4988..6502)
                     /gene="norR"
                     /locus_tag="N7A07_000478"
     CDS             complement(4988..6502)
                     /gene="norR"
                     /locus_tag="N7A07_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU2475212.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6689..8128
                     /gene="norV"
                     /locus_tag="N7A07_000479"
     CDS             6689..8128
                     /gene="norV"
                     /locus_tag="N7A07_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU2475213.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEKMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8125..9258
                     /gene="norW"
                     /locus_tag="N7A07_000480"
     CDS             8125..9258
                     /gene="norW"
                     /locus_tag="N7A07_000480"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU2475214.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(9386..11638)
                     /gene="hypF"
                     /locus_tag="N7A07_000481"
     CDS             complement(9386..11638)
                     /gene="hypF"
                     /locus_tag="N7A07_000481"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU2475215.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11791..12318)
                     /gene="hydN"
                     /locus_tag="N7A07_000482"
     CDS             complement(11791..12318)
                     /gene="hydN"
                     /locus_tag="N7A07_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU2475216.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12467..13477)
                     /gene="ascG"
                     /locus_tag="N7A07_000483"
     CDS             complement(12467..13477)
                     /gene="ascG"
                     /locus_tag="N7A07_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU2475217.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13737..15194
                     /gene="ascF"
                     /locus_tag="N7A07_000484"
     CDS             13737..15194
                     /gene="ascF"
                     /locus_tag="N7A07_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU2475218.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            15203..16627
                     /gene="ascB"
                     /locus_tag="N7A07_000485"
     CDS             15203..16627
                     /gene="ascB"
                     /locus_tag="N7A07_000485"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU2475219.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16751..17221)
                     /gene="hycI"
                     /locus_tag="N7A07_000486"
     CDS             complement(16751..17221)
                     /gene="hycI"
                     /locus_tag="N7A07_000486"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU2475220.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17214..17624)
                     /gene="hycH"
                     /locus_tag="N7A07_000487"
     CDS             complement(17214..17624)
                     /gene="hycH"
                     /locus_tag="N7A07_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU2475221.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17621..18388)
                     /gene="hycG"
                     /locus_tag="N7A07_000488"
     CDS             complement(17621..18388)
                     /gene="hycG"
                     /locus_tag="N7A07_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU2475222.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18388..18930)
                     /gene="hycF"
                     /locus_tag="N7A07_000489"
     CDS             complement(18388..18930)
                     /gene="hycF"
                     /locus_tag="N7A07_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU2475223.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(18940..20649)
                     /gene="hycE"
                     /locus_tag="N7A07_000490"
     CDS             complement(18940..20649)
                     /gene="hycE"
                     /locus_tag="N7A07_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU2475224.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20667..21590)
                     /gene="hycD"
                     /locus_tag="N7A07_000491"
     CDS             complement(20667..21590)
                     /gene="hycD"
                     /locus_tag="N7A07_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU2475225.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21593..23419)
                     /gene="hycC"
                     /locus_tag="N7A07_000492"
     CDS             complement(21593..23419)
                     /gene="hycC"
                     /locus_tag="N7A07_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU2475226.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23416..24027)
                     /gene="hycB"
                     /locus_tag="N7A07_000493"
     CDS             complement(23416..24027)
                     /gene="hycB"
                     /locus_tag="N7A07_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU2475227.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24152..24613)
                     /gene="hycA"
                     /locus_tag="N7A07_000494"
     CDS             complement(24152..24613)
                     /gene="hycA"
                     /locus_tag="N7A07_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU2475228.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            24825..25175
                     /gene="hypA"
                     /locus_tag="N7A07_000495"
     CDS             24825..25175
                     /gene="hypA"
                     /locus_tag="N7A07_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU2475229.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25179..26051
                     /gene="hypB"
                     /locus_tag="N7A07_000496"
     CDS             25179..26051
                     /gene="hypB"
                     /locus_tag="N7A07_000496"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU2475230.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26042..26314
                     /gene="hypC"
                     /locus_tag="N7A07_000497"
     CDS             26042..26314
                     /gene="hypC"
                     /locus_tag="N7A07_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU2475231.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26314..27435
                     /gene="hypD"
                     /locus_tag="N7A07_000498"
     CDS             26314..27435
                     /gene="hypD"
                     /locus_tag="N7A07_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU2475232.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27432..28442
                     /gene="hypE"
                     /locus_tag="N7A07_000499"
     CDS             27432..28442
                     /gene="hypE"
                     /locus_tag="N7A07_000499"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU2475233.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28516..30594
                     /gene="flhA"
                     /locus_tag="N7A07_000500"
     CDS             28516..30594
                     /gene="flhA"
                     /locus_tag="N7A07_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU2475234.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(30631..30984)
                     /gene="ygbA"
                     /locus_tag="N7A07_000501"
     CDS             complement(30631..30984)
                     /gene="ygbA"
                     /locus_tag="N7A07_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU2475235.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(31061..31195)
                     /locus_tag="N7A07_000502"
     CDS             complement(31061..31195)
                     /locus_tag="N7A07_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475236.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            31271..33832
                     /gene="mutS"
                     /locus_tag="N7A07_000503"
     CDS             31271..33832
                     /gene="mutS"
                     /locus_tag="N7A07_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU2475237.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            33938..34594
                     /gene="pphB"
                     /locus_tag="N7A07_000504"
     CDS             33938..34594
                     /gene="pphB"
                     /locus_tag="N7A07_000504"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU2475238.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(34635..34871)
                     /locus_tag="N7A07_000505"
     CDS             complement(34635..34871)
                     /locus_tag="N7A07_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU2475239.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(34882..36309)
                     /locus_tag="N7A07_000506"
     CDS             complement(34882..36309)
                     /locus_tag="N7A07_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU2475240.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(36309..36902)
                     /locus_tag="N7A07_000507"
     CDS             complement(36309..36902)
                     /locus_tag="N7A07_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU2475241.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            37049..37456
                     /locus_tag="N7A07_000508"
     CDS             37049..37456
                     /locus_tag="N7A07_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU2475242.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(37576..38568)
                     /gene="rpoS"
                     /locus_tag="N7A07_000509"
                     /pseudo
     CDS             complement(37576..38568)
                     /gene="rpoS"
                     /locus_tag="N7A07_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(38631..39677)
                     /gene="nlpD"
                     /locus_tag="N7A07_000510"
     CDS             complement(38631..39677)
                     /gene="nlpD"
                     /locus_tag="N7A07_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU2475243.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFY
                     IAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVI
                     KPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVS
                     STSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNAL
                     RGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRY
                     KGKSVNPLRYLPQR"
     gene            complement(39862..40488)
                     /gene="pcm"
                     /locus_tag="N7A07_000511"
     CDS             complement(39862..40488)
                     /gene="pcm"
                     /locus_tag="N7A07_000511"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU2475244.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(40482..41243)
                     /gene="surE"
                     /locus_tag="N7A07_000512"
     CDS             complement(40482..41243)
                     /gene="surE"
                     /locus_tag="N7A07_000512"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU2475245.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(41224..42273)
                     /gene="truD"
                     /locus_tag="N7A07_000513"
     CDS             complement(41224..42273)
                     /gene="truD"
                     /locus_tag="N7A07_000513"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU2475246.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(42270..42749)
                     /gene="ispF"
                     /locus_tag="N7A07_000514"
     CDS             complement(42270..42749)
                     /gene="ispF"
                     /locus_tag="N7A07_000514"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU2475247.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(42749..43459)
                     /gene="ispD"
                     /locus_tag="N7A07_000515"
     CDS             complement(42749..43459)
                     /gene="ispD"
                     /locus_tag="N7A07_000515"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU2475248.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(43478..43789)
                     /gene="ftsB"
                     /locus_tag="N7A07_000516"
     CDS             complement(43478..43789)
                     /gene="ftsB"
                     /locus_tag="N7A07_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU2475249.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(43983..44306)
                     /gene="ygbE"
                     /locus_tag="N7A07_000517"
     CDS             complement(43983..44306)
                     /gene="ygbE"
                     /locus_tag="N7A07_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU2475250.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(44356..44961)
                     /gene="cysC"
                     /locus_tag="N7A07_000518"
     CDS             complement(44356..44961)
                     /gene="cysC"
                     /locus_tag="N7A07_000518"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU2475251.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(44961..46388)
                     /gene="cysN"
                     /locus_tag="N7A07_000519"
     CDS             complement(44961..46388)
                     /gene="cysN"
                     /locus_tag="N7A07_000519"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU2475252.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(46390..47298)
                     /gene="cysD"
                     /locus_tag="N7A07_000520"
     CDS             complement(46390..47298)
                     /gene="cysD"
                     /locus_tag="N7A07_000520"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU2475253.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            47550..48587
                     /gene="iap"
                     /locus_tag="N7A07_000521"
     CDS             47550..48587
                     /gene="iap"
                     /locus_tag="N7A07_000521"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU2475254.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        11519 a        13407 c        12852 g        11075 t
ORIGIN      
        1 tgccgtttgg taataaaaca ataaatcctg aaggagagaa caatgataga aaccattact
       61 catggtgcag agtggtttat cgggctgttc caaaagggcg gagaggtgtt taccgggatg
      121 gtgaccggca ttcttccgct gttgattagc ctgctggtta tcatgaacgc actgattaat
      181 tttatcggtc agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa ccctgtttcc
      241 cgttacctac tgttaccgtg cattggcacg tttgtctttt gcaatccgat gaccttaagc
      301 ctgggtcgct ttatgccgga aaagtacaaa cccagctact acgcggcggc ctcttatagc
      361 tgccactcaa tgaatggcct cttcccccat atcaaccctg gcgaactgtt tgttatcttg
      421 gcattgccag cggtctgaca acgctgaacc tgccacttgg cccactggcg gtgagctatc
      481 tgctggttgg tctggtcacc aacttcttcc gcggctgggt gaccgatctg accaccgcta
      541 tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcatctggca ggagcaacat
      601 catgacgcat attcggatcg aaaaaggaac ggggggctgg ggcggcccgc ttgagctgga
      661 aaccacgccg ggcaaaaaaa tcgtctatat caccccgccg gtacccggcc tgcgattgtc
      721 gacaaactgg cacagcttac tggctggcag gctattgacg gatttaaaga aggtgaaccc
      781 gcggaggcgg aaattggtgt cgcggtaatc gactgtggcg gcacattacg ctgcggcatt
      841 tatccgaaac ggcgtattcc caccattaat atccactcga cgggcaagtc cggtccactg
      901 gcgcagtaca ttgtggaaga tatttatgtc tctggcgtaa aagaagaaaa catcactgta
      961 gtgggtgatg cgacaccaca accctcttcc gtgggccgtg actatgacac cagcaagaaa
     1021 atcaccgaac aaagcgatgg tttactggcg aaggtgggaa tgggtatggg ttctgccgtt
     1081 gccgtgttgt ttcaatctgg tcgtgacacc atcgacactg tattaaaaac cattcttccg
     1141 tttatggcat tcgtctcggc gctcattggc atcattatgg cttctggcct tggtgactgg
     1201 attgcccacg gccttgctcc gctggcgagc catccactgg gtctggtcat gctggcgctc
     1261 atctgctcct tcccgctgct ttcacctttc ctcggcccag gcgcagttat cgcacaggtt
     1321 atcggcgtat tgattggcgt gcagattggt ctcggcaata ttccgccgca tctggcttta
     1381 cctgcactgt ttgccatcaa cgcgcaggcg gcctgcgact tcattccggt cggtttgtcg
     1441 ctggcggaag cccgtcagga cacggttcgc gtcggtgtcc cttctgtact ggtgagccgc
     1501 tttttaaccg gcgcgccgac tgtactgatc gcctggtttg tctccggttt tatctatcaa
     1561 tagaggctga aacatgaccg ttatttatca gaccaccatc acccgtatcg gcgcgagtgc
     1621 cactgacgcc ctcagcgacc agatgctcat cacctttcgt gaaggcgcgc cagcggacct
     1681 cgaagagtat tgcttcattc attgccacgg cgagttgaaa ggtgcactcc atcccggttt
     1741 gcaatttacg ctcgggcagc atcgctatcc ggtgaccgct gttggcagcg tggcggaaga
     1801 caaccttcgc gaactgggtc atgtcaccct gcgcttcgat ggtttaaacg aagcggaatt
     1861 tccgggcact gtccatgtgg caggccctgt acccgacgat atcgcgccgg gatcggtttt
     1921 gaagtttgaa tctgttaagg agtaaaaaat gaatcaggtt gccgttgtca tcggtggtgg
     1981 gcaaacctta ggcgcgttcc tgtgccacgg tctggctgcc gaggggtatc gcgtcgcggt
     2041 tgtcgatatt cagagcgaca aagccgcaaa tgtggcacaa gaaattaacg ccgaatatgg
     2101 tgaaggaaca gcgtatggtt ttggtgctga cgccactagc gagcaaagcg ttctggcgct
     2161 ctctcgtggg gtagatgaaa tctttgttcg tgtggattta ctggtctaca gcgccggaat
     2221 tgcaaaagca gcctttatca gcgacttcca gctcggcgat tttgaccgtt cgctacaggt
     2281 gaatctggtg ggttatttcc tgtgtgcgcg tgaattttcg cgtttgatga tccgcgatgg
     2341 gattcaggga cgcattattc agatcaactc gaaatccggc aaagtgggca gcaaacacaa
     2401 ttctggttat agcgccgcga aatttggtgg cgtagggctg actcaatcac tggcgctgga
     2461 tctggcggag tacggcatta cggtgcattc actgatgctc ggtaacctgc tgaaatcgcc
     2521 gatgttccag tcgctgttgc cacaatatgc caccaagctg ggtatcaaac cggatcaagt
     2581 cgagcagtat tacatcgaca aagtaccgct caaacgcggc tgcgattatc aggatgtgct
     2641 gaatatgctg ctgttctacg ccagtcctaa ggcgtcgtac tgtaccggac agtcgatcaa
     2701 tgtcaccggc ggtcaggtga tgttctgatc aacagcggag atccgttaag gatctccgtg
     2761 agactaatag aatgcctgat gcgctacgct catcaggcat acaggacttc cgccactaca
     2821 ttaaggaaaa gttatggtat ccgcactcat cactgtcgcc gttatcgcct ggtgtgcgca
     2881 actggcctta ggcggctggc aaatttctcg ttttaaccgt gccttcgaca cactatgcca
     2941 gcaagggcgg gttggcgtgg gccgttccag cgggcgcttt aaaccgcggg tcgtggtcgc
     3001 catcgccctg gacgatcagc agcgcatcgc cgacaccttg tttatgaaag gactgaccgt
     3061 cttcgcccga ccgcaaaaaa ttcccgcaat taccggtatg catgcgggtg atttacagcc
     3121 cgatgtgatc tttccccatg atccactatc acagaatgct ctatcattgg cgcttaaact
     3181 gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg aagttatcat tttgaaacct
     3241 aaatcaggta atcacgccca tgaaacctcg tcagcgtcaa gccgccattc tggagtatct
     3301 gcaaaagcag ggaaaatgct cggttgaaga attggcgcaa tattttgaca ccacaggcac
     3361 aactattcgc aaagatctgg tcattctgga acatgccgga accgtcattc gtacttatgg
     3421 cggagtggtg ttgaataaag aggaatccga tccgcctatc gatcataaaa cactcatcaa
     3481 cacccacaag aaagagctga tagcagaagc tgccgttggt tttattcatg atggcgattc
     3541 gatcattctt gatgctggca gtaccgtttt gcagatggtg cctctgctct cgcgctttaa
     3601 taacatcacg gtgatgacca acagcctgca tatcgtcaat gcgttatctg aactggataa
     3661 tgaacaaact atcctgatgc caggcggaac gtttcgtaaa aaatcggcct catttcacgg
     3721 gcagctggca gagaatgcct tcgagcattt caccttcgac aaattattca tgggtaccga
     3781 cggcatcgat ctcaatgcgg gcgtaaccac cttcaacgag gtctataccg tcagtaaggc
     3841 aatgtgcaat gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt ttggccgtaa
     3901 aagccccaac gtagtttgca gtcttgaaag cgtcgataag ctgattaccg acgcagatat
     3961 cgatccggcg tttcgtcagg cgctggaaga gaaagggatc gatgtgatca taaccggaga
     4021 gagcaatgag tgaagcacta ctgaacgcgg gacgtcagac gttaatgctg gaattgcagg
     4081 aagcaagccg tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc
     4141 tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa atcgggccac attggtaaga
     4201 aaatcgccgc aacgcttgcc agtaccggca caccggcttt ttttgtccat ccggcagaag
     4261 cgctgcatgg cgatctgggg atgatcgaaa gccgcgatgt gatgctgttt atctcttact
     4321 ccggtggggc gaaggaactg gatctgatta ttccgcgtct ggaagacaaa tctatcgcgc
     4381 tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct ggcggcgaaa gcggtgctgg
     4441 atatctccgt agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc agcaccgtca
     4501 ataccctgat gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc ggatttaatg
     4561 aagaagattt tgcccgctcc caccctgctg gagcactggg cgctcgcttg ctgaataaag
     4621 tgcatcatct gatgcgccgt gacgatgcca tcccacaggt ggcgttaacc gccagcgtga
     4681 tggatgctat gctggaactc agccgcaccg gtctggggct ggtggcggta tgtgacgctc
     4741 aacaacaggt acaaggcgtc tttaccgacg gcgatttacg tcgctggctg gttggcggcg
     4801 gcgcactcac aacgccagtc aatgaagcga tgacggtcgg cggcaccacg ttgcaatcgc
     4861 aaagtcgcgc catcgacgcc aaagagatcc tgatgaagcg caaaatcact gccgcaccgg
     4921 tggtggatga aaacggcaaa ctcaccggcg caataaacct gcaggatttc tatcaggccg
     4981 ggattattta atccttcaat cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg
     5041 tttccagcat ccgcgcgcag gcagcccagt tgtgatgatt ttgtgccagt gcctgacgaa
     5101 tagtttcacg ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg
     5161 ccgccacttc tggcggcggc aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa
     5221 tcacttcatc gccgctgcgg gtggctctcg ccagcactac cgcccgatga atagcatgtt
     5281 ccagttcgcg cacgtttccc ggaaaactgt agtgttgcag taaatttcgc gccccggcac
     5341 ttaataccac gcgggagagc ccctgccgca aacgacactg ctcgcagaaa taccccgcca
     5401 gcagaatgac atcatcgccc cgctcacgca gcggcggcac cgaaagtgga aacacactca
     5461 ggcgatgaaa caaatcggcg cggaatcgcc ctgccagcac ctcttcgcgt aagtcgcggt
     5521 tagtcgccgc cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa
     5581 tatcgccata ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac aactcgccga
     5641 tctcatcgag aaacagcgtg ccgttatccg ccatttcaaa cttcccactg cggttactga
     5701 tagcgccagt aaacgctcct ttcacatgcc cgaacaactc actttccgcc acactttccg
     5761 gcagtgcggc acagttgaga tagaccagcg gattcaccgc ccgtggcgag gcctcatgaa
     5821 tcgctttcgc caccagctcc ttaccggttc ccgtctcacc gctgatcagg acgttgagat
     5881 cggacgccgc cacaatctca atctcttttt tcagttgcgt catgcctggc gacagaccga
     5941 tcatctgcgt ctgtttcacc gcttcaaatg gagcggcatc gcccggcagc atattctggc
     6001 tttccagttg ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc gcggcaatca
     6061 ggcgtaactc ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg tcgagcgtca
     6121 atgcgccgat caggttttgc ccggcaaaca gcggcagacc aacgcaggcg tgaaccttca
     6181 gactctcctg ccctggaatc aaaccgtcat agggatcggg caattcgctg tctgcgggaa
     6241 agcgcaccac atcacccgcg cgggcaatcg cttccagccg tggatgacct tccagcgcaa
     6301 agcgtctacc gagtacatcc ttcgccagcc cgtcgatggc aagcggaata aactgccgcg
     6361 agtcgtaacg tagcaacgca gacgcatcgc actccagcac ctgacgcagc gtggtgatca
     6421 ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca
     6481 gcacatcaac ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat
     6541 atcgaccatt ggattgatag tcattttgac tactcattaa tgagcataat tttatttata
     6601 gagtaaaaac aatcagataa aaaactggca cgcaatctgc aattagcaag acatcttttt
     6661 agaacacgct gaataaattg aggttgctat gtctattgtg gtgaaaaata acattcattg
     6721 ggttggtcaa cgtgactggg aagtacgaga ttttcacggc acggaatata aaacgctgcg
     6781 cggcagcagc tacaacagct acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt
     6841 cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg atctggcgga
     6901 tatcgattac atcgtgatta accatgcaga agaggaccac gccggggcgc tgaccgaact
     6961 gatggcacaa attcccgata cgccgatcta ctgtacagcc aacgctatcg actcgataaa
     7021 tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggcg acacgctgga
     7081 tatcggcaac ggcaaacagc tcatttttgt cgaaacacca atgctgcact ggccggacag
     7141 catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt tcggtcagca
     7201 ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt ttgagcagtg
     7261 ccagcgttac tacgccaata tccttacgcc gttcagccgt ctggtaacgc cgaaaattac
     7321 cgagatcctg ggctttaact taccagtcga tatgatagcg acctctcacg gcgtggtatg
     7381 gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaaa tgggcggctg attatcagga
     7441 agacagaatc accattttct acgacaccat gtcgaataac acccgcatga tggctgacgc
     7501 tatcgcccag gggattgcgg aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc
     7561 ccgaagcgat aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg
     7621 cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg agaagatgac
     7681 cggattacgc ttccgtaaca aacgcgccag tgctttcggc tctcacggct ggagcggcgg
     7741 tgcggtggat cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct
     7801 gaaagcgaaa tggcgaccag accaggacgc tctggagtta tgccgtgaac acggtcgcga
     7861 aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata cggtagttaa
     7921 agaagaaacc tctgccacca cgacggctga cctcggccca cggatgcagt gcagcgtctg
     7981 ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc caggaacgcc
     8041 gtggagtgaa gtcccggata acttcctgtg cccggaatgc tccctcggca aagacgtctt
     8101 tgacgaactg gcatcggagg caaaatgagc aacggcattg tgatcattgg ttcgggcttc
     8161 gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccagtattcc attaaccctg
     8221 attgccgccg acagtatgga tgagtacaac aaacctgacc tcagccatgt tatcagtcag
     8281 gggcaacgtg ccgacgacct tacccgccag acggcgggtg aatttgccga gcagtttaat
     8341 ctgctcctgt ttccgcaaac ctgggtgacg gatatcgatg ccgaagcccg tgtggtgaaa
     8401 agccagaata atcagtggca atacgacaag ctggtgctgg caaccggtgc cagcgccttt
     8461 gtcccgccag tgcctgggcg tgagttaatg ctgacgttaa atagtcagca agagtatcgc
     8521 gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgttggcgg tggtttgatt
     8581 ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaaa tggttacgct aatcgacaac
     8641 gctgccagta ttctggcgtc gttaatgcca ccggaagtaa gcagtcgctt gcagcatcgg
     8701 ttgacggaga tgggcgttca tctgctgttg aaatctcagt tgcaggggct ggaaaaaacg
     8761 gattctggca ttctggcaac gctggaccac cagcgcagca tcgaagtgga tgcggtaatt
     8821 gccgccaccg gactgcgccc ggaaaccgcc ctggcacgac gcgccgggct gacgattaat
     8881 cgcggcgttt gcgtcgatag ttatctgcaa accagtaatg ccgatattta cgcgctgggc
     8941 gattgcgcgg aaattaacgg tcaggtattg ccgttcctgc agccgattca acttagcgcg
     9001 atggtgctgg caaaaaatct tctcggcaat aacacgccgc tgaaactacc ggcgatgctg
     9061 gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca gcgtcaggat
     9121 ttacgctggc agattaatac cgaacgccag ggaatggtgg cgcgcggtgt tgacgatgca
     9181 gaccaacttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt tggattgttg
     9241 aaaacgttgc cggtgtaggt gggctactgt gcctaaaatg tcggatgcga cgctggcgcg
     9301 tcttatccga cctacgggga cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg
     9361 caatggtgtc caaatgcaac acgttttatc cgttctggac ttcacccgct aaccaacgcg
     9421 ccgcagcaat aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg
     9481 gaaacagcaa tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat
     9541 gaataacccc gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca
     9601 tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt tgattaaccg
     9661 gtgcctgcca gttcagccac tgctgccaga aagtggcgag atccagttga ttgtccaccc
     9721 gcggcatcgt caccggatgc gtcactccgt cgcatgaggc tgctagcgcc tccagagcac
     9781 aagccgcttc accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccacag
     9841 catcgaacaa acgcccacac gatgacgcca gcggcgcgtt aattccacgc tcaatggccc
     9901 gcgccagtac gctccagttt tgctgttgca cacttgccgt ttcagggtaa ttctgccact
     9961 ccggcacaaa gcgcaggcac tgcgccagca ggttacgcca cggctgcttc gccgccaaat
    10021 cgccacccgg aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca
    10081 cccgcaggca ctcgccgccc cacaaagcgc cgttctcccc cataccgata ccgtcgagcg
    10141 tcaaagcaat gacatcaccg ccatccagcg gccactgatg ctctgccaga cacgccgctg
    10201 cgtgggcatg atgatgcagc accgtttgcg tcggcagatt catttcgctc gcccactggc
    10261 aggagacata gcccggatgt gcgtcatgca caacgtattg cggagtgaaa ttgtagatgt
    10321 tttgcatcag gcgtaacgct tcgcgccact gcgtctggat gccatcgtca cttaaatcgc
    10381 ccagatgctg gctcaacacc acttgttcac cgcgcaccag gcagaaggta tttttcagat
    10441 ccgcgccgag acacagtaca ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat
    10501 ccggcacata cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat
    10561 ccatccgctg cacaatgtcg cggttgtgta ttaagaatcc gtcggcaatg ccctgcaaat
    10621 cctccagcgc ctgttcgttg ctgatagccg gtggtttacc gctcaggttg ccagaggtca
    10681 tcaccagcgg gcattgtagt tcctgtaaca gcaaatgctg aagcggattc gcaggcaaca
    10741 ttaccccgac ttcgttaagg ccaggggcga tatcatcaca aagctcagga acgtattttt
    10801 tatccaccag cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta
    10861 aaccgtcagc cactggcaac atgactgcca gcggtttcgc cgggcgatgt ttgcgcgccc
    10921 gaagtgtcgc caccgcgtta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc
    10981 ctttgatggc gacaatgttg cccattttta actgtgcgat agccgcctgt aatgccgcct
    11041 cttgttctgc atgttcacca tgacttaccc attcaagata cgggccacac tccgggcagg
    11101 ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg gtactcttta tcacaggctg
    11161 gacacagcgg aaacgccgcc atcacggtaa acgggcgatc gtaaggcatg gcgcgaataa
    11221 tggtgaagcg cgggccgcag tgggtacagt tgataaatgg ataacgataa cgccgttcgc
    11281 ctggggtatt catttcggca aggcaagcag ggcaagtggc ggcatcggga acaatttgcg
    11341 tattcatggc gccgcccgcg ctctggcgga tggtgaactc agtgggcagt tgtgaccaga
    11401 taaacggttc acgctcaacg ctatcaatac gcgccagcgg cgggcagtgc tgatgcaatt
    11461 gaacaagaaa cgtttccggg tcttccagca gccggacttc tacgccatcg ccgtcattac
    11521 agacatcgcc gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga
    11581 cgccctgcac tttgcctcga atacgcagtt ggacaccgca agatgtgttt tttgccattg
    11641 agttattccc gccatcatga attgcgtaac ccgtccagcc ggacacgaca gcgtcgcatc
    11701 cggcagtcac aggtcggcga taccgcccgc tccgtattct acgaatattt ccgggaattc
    11761 ctttgatgcc agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt
    11821 ttctgcgctc agttgttcaa gtttattgcg atcgacacaa attagcgcat gggtcgggca
    11881 agccgccata cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc
    11941 ggctttgtca gcccgtacat tcagccctgc gccgctgttg cgaatcaccg gacgtaccac
    12001 cacttccatc gcgccatacg ggcaagccac aacgcaggtt ttgcaaccaa tgcaacgttc
    12061 ctgcatcaca tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc
    12121 gcacggtgca tcttcacact gacggcaaac tgtcgccgtg gaaatgttca cacctttaat
    12181 gacatggata cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct gattttcctg
    12241 atgagaaacc acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc
    12301 aatgatgaaa cggttcatca aattctccag caatgacagt taatgcgccg atacattcac
    12361 aaatcatgcc agtttttaat ttgctgttat ttaaggaaat taatttctgt aatgcaggaa
    12421 aaaagatgtc atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaagag
    12481 caatgagtga gtcgcggcgg atcagttttc cgctgaaggt tttcggcggt gagaaatccc
    12541 cgccatcgag cataaaaatc agccgtccaa taatttcctg aatcatctca gtcaccggaa
    12601 tttttacgct ggagagcgcc ggaacgatat agggggcaat tgcgatatca tcgaatccga
    12661 taactgacac ctgctctggc accgctacgc cgcgcttgtg taacgctttc atcgcaccta
    12721 tcgccatatc gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaacg
    12781 tttctacccc ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat
    12841 tgagcgcaat accatgctgc gccagcgcgt ctttatatcc ggcaagacgt tcaacgctgg
    12901 tgggggaatc catcgagccg gtaagaaaag caatctcctg atgcccggcg tttatcaact
    12961 ctgccacggc gttaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctatttt
    13021 tgcgcaggcg gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcga
    13081 tctcatccac acttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca
    13141 gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact
    13201 gccgcccttt ctcttccgcc attcgcgcgg catgaaacag taattcacta aaataaatgc
    13261 cgtggtaaag cgtgttagtc actaccagcc ccagcgtctg ggtacttttc gccgacagat
    13321 tgcgcgccag caagtttgga cggtaaccgc tctcttctac cgcctgaaac acgcgatctt
    13381 tagtctcctg gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa
    13441 ccccggcgcg cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa
    13501 tcaacgcagt gtactgcacc gtttaccgat tgtccttgcg caatcggcgg gaaaaatatt
    13561 caggtgaccg gtttcacaaa tataaaaaat gaacaattaa ctctcttgct tatttggtta
    13621 caactattca tgattttgtg aaaccggttt cttaattccg tttcagcatc ggcatttttc
    13681 cgtcacgtcg actgataaca actacatctt ccctactgat aacaggataa aatccgatgg
    13741 ccaaaaatta tgcggcgctg gcacgctcgg tgatagcggc actgggcggc gttgataaca
    13801 tctcggcggt cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat gatgcgctta
    13861 tcgacagccc gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc
    13921 agtgtcaggt gattattggc aataccgttt cacaagcctt tcaggaagtc gtcagcctgc
    13981 tgccgggaga tatgcagccc gccctgcccg tgggtaaacc caaactcacg ctgcgtcgca
    14041 ttggtgcggg gatcctcgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta
    14101 tcggcggatc gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa
    14161 aaggatcgcc gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc
    14221 cgctgatggt cgcggcttcg gccgccatca aatttaaaac caatatgtcg ctggcgattg
    14281 ccatcgcggg tgtgctggta catccgagtt ttattgaact gatggcgaaa gcggcccagg
    14341 gtgaacatgt cgaatttgcc ctgattccag tcaccgcggt gaaatacacc tacacagtga
    14401 tcccggcgct ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta
    14461 caccggcggt gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc
    14521 tggcaatcct gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg
    14581 tttacaccat tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc
    14641 ctctgctggt aatgaccggg atgcaccgcg tctttacgct aaccatcatt cagaccattg
    14701 ccgaaaccgg caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg
    14761 gcggttcatc actggcggtg gcgtggaaaa cgaaaaactc ggaactgcgc cagactgcgc
    14821 tggctgcggc ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg
    14881 cgatccgcct gaaacgtccg cttatcgcca gtcttatcag cggttttatt tgcggcgcgg
    14941 ttgccggtat ggcggggctt gccagccact caatggcagc gccggggcta tttaccagcg
    15001 tgcagttctt cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc
    15061 tggcggtggt gctgtcgttt atcctcacac tgttgctcgg ctttgaggat attcctgttg
    15121 aggaagcggc tgccgaggcg cgaaagcatc agagcgcaca accgaccgtc gccaaagaag
    15181 taagtcttaa ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc
    15241 gcttgccgcc aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaaggac tgacaacggt
    15301 cgatatgatc ccacacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt
    15361 tcagttgcgc gatgacgagt tttatcccag ccatgaggcg acgggttttt atcatcgtta
    15421 taaagaagat atcgccctga tggcagagat gggattcaaa gtgttccgta cctcaattgc
    15481 ctggagccgc cccttcccgc agggtgatga actgacgccc aaccagcagg gcattgcttt
    15541 ttaccgttct gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacattgtg
    15601 ccacttcgat gtgccgatgc agctggtcac cgaatatggc tcctggcgta accgcaagct
    15661 ggtggagttt ttcagccgct acgccaggac ctgctttgaa gcatttgatg gtctggtgaa
    15721 atactggcta accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg
    15781 gctggtgttt gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca
    15841 gctggttgcc agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt
    15901 gggctgtatg ctggcgggcg gtaacttcta cccttacagt tgcaagccgg aagatgtctg
    15961 ggcggcgctg gagaaagatc gggaaaacct gttttttatc gatgtgcagg cgcggggcgc
    16021 gtatccggct tactctgctc gcgtattccg cgaaaaaggg gtaaccatcg acaaagcacc
    16081 gggcgatgat gagatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc
    16141 gcgctgcgcc tcggcggaga tgaacaccaa caatagcagt gcggcgaacg tagtgaaatc
    16201 gctgcgtaac ccgtatctac aggtgagcga ctggggctgg ggaattgatc cactcggtct
    16261 gcgtatcacc atgaacatga tgtacgaccg ctatcagaag ccgctgtttc tggtggaaaa
    16321 cggcctcggc gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat
    16381 cagctattta cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct
    16441 gatgggctac accacatggg gctgtattga tttagtttcc gcctctacgg gtgaaatgag
    16501 caaacgctac ggttttgtct ttgttgaccg tgacgacgca ggcaacggca cgctgacgcg
    16561 cacacgtaag aaatcgttct ggtggtataa aaaagtgatt gccagtaatg gggaagattt
    16621 agagtaggta acctactgtc gcactaacca ggaaggcccg ataagcgttg cgcatcgggc
    16681 aatctggggt ttgctatcag ttctgattcc ctcatcaaga acatccctgt cctgattact
    16741 tattgaaaaa ctactcttct tccaccgcta actgcgcgaa gccgccgttc ccttcccagc
    16801 cttccagtcg ttgataaacg gtttctaccg catctttaat cggctgggtc atcgggtagt
    16861 aaaagccgac gatatccggc tggatgccga ggaaaatcac ttcgccaata tcttctttca
    16921 actgatcgat aaggtagttc aacggcatgt tatgggtagt catcataaac atctcggcga
    16981 tatcatccgg gtcgatgatg cggatttcgc cggggtttag ccccatatcc gtggcgtcga
    17041 caatcagcag ccgtgtcggg cgcagttcgc ggatagcgac aatgtcgttt tccggtgcgc
    17101 taccgccgtc aatcaccacc cagttacctt tcggcgcggc ggcgcacttt tccgccagca
    17161 gcggacctgc gccatcatca cccatcatgc tattgccaac acagagtaaa acgtcagtca
    17221 cgcagtctcc tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt
    17281 aacagcattg tactccattc ctgctgctgc gaagtttgcg ctgcccgggc tttatcgaat
    17341 gcattggcca gcatcggcac atggttaatg tcgatgacga tctcaccata tttcggcaca
    17401 ccttccattt tgcggcgggc ttcgctgccc gcctccagag tggcaatcca tgccagatat
    17461 tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga taaccccaag gtggtgacca
    17521 atcgccaggc tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca
    17581 ataaatttac ggctcagttg actgaacacc accttttcac tcatcggata cgcgcctctt
    17641 caacaacatg attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca
    17701 gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt aaggtaatca tcggcaatct
    17761 gacgaccgta acgataaccc gccagtcgac gtgcttcgcg atccactttc acacgcagcg
    17821 gctgcaccat atcgccatgc aggatctccg ccggttgttc atcctgctca cccggcccac
    17881 gggcgtgaat tttctgctcc agcaggccga gcgccattgc aaagccgtac agcgtggcgg
    17941 ctggcgttgg cgggcagcca gggatataaa catccaccgg gacgatttta tccgtaccgc
    18001 cccacacgca gtagagatcg tggaagatcc cgccgctgtt accgcaggca ccgtaggaga
    18061 tacaaatttt cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac
    18121 gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg cggtgaagga acgactttaa
    18181 tgccgaagcg ttctgcatcg aacagcggcg acaaagtggc gaaaatttcg atttcgcagc
    18241 cgttgcagcc gccgcagtcc acgcggtaaa cgtaggcaga acgtttgatt tttttcagta
    18301 acgacgcctt catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg
    18361 cgtcacgggg gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc
    18421 aatacggtcg gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct
    18481 ttcgcggtgg ttttccgcgc ggctgtcgcc attgtgctta agcagcgcaa tggcgtagtc
    18541 gatctctttc tggacggcga aaggacgatt gcagacgcgg cagttgcaca gcgcgaagcg
    18601 ggactgttgc aggaagtctt ctttcttcca caccgccagt tcgtactctt gcgacagttt
    18661 gattgccgcc gtcgggcaga cttcttcgca gcgtccgcag aagatgcagc gcccgagatt
    18721 gaactcccac gccagctcac cggtggcgag gtcagtttca accgttaagg cgtttgacgg
    18781 gcaggcattg acgcaggccg cgcagccgat gcactgctgc gggttctgct ctggcttacc
    18841 acggaagttt ttatcaaccg caatcggttc cagcggataa gacgaggtcg ccgtgccggt
    18901 tttgatgact tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt
    18961 acgctcaatg ctgtaacgct cgagttcttt gtacggcacc actttgctct ttttcttacg
    19021 cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa gggtccagac taccgataat
    19081 cagcggcgca tcagaaacgg tgttgccgcg cagcatgtag cgcagggtcg gccagttcgc
    19141 gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccgg tcatgctcca
    19201 gtggatatca tcgccgcgcg gcgcttcggc aaagcccagc gcaaaacggt gcggaatgta
    19261 ggtaaagcct tccaccatca gcgggccgcc cggcaggtta tccagaccgt agtcgatcat
    19321 gttcagcgcg gtatagactt cgttaatacg cactttcaga cgcgaaataa cgtcgcagcc
    19381 ctgctcgctg tggacttcca ttggcagcag gccataacca acaaacgggt gatcggcgcg
    19441 ggtatcgcgg gcgtgaccgc tggcacggac catcgggccg acgttactga agtcgcgagc
    19501 gatttccggg tccagacgac caatgccgac agtgcgctgt tccatgttcg gcgtactcag
    19561 cagcacatcc accagctcct gcacttcacg acgcatctgt tgcgccagtt gacgggtctg
    19621 gatcatgtca tctttcagca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt
    19681 tttacgcgca ccggtaagga tctctgccat tttcatggag gtttcacgca cgcggaagaa
    19741 ctgcataaag ccggagtcaa agccggtaaa gtgacaggcg aggccgaggt tgagcagatg
    19801 cgagtgcaga cgttctacct ccagcagaat ggcgcggatc atctgcgcac gttctggcac
    19861 ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg gtgctgtgag caaaaccgca
    19921 gatcccgcac acacggtcag agaggaaggt cacttcgtta taacccatgc gggtttctgc
    19981 cagtttttcc atgccgcgat ggacatagaa caggcggtag tcggcgtcga taatgttttc
    20041 gccatcgacg aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat
    20101 cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcatc
    20161 ggtggtcggt gccggacgct gacgataatc catgctgtct ttacgcagcg gataaagttc
    20221 atccggccag tcatccggca gcaccagacg acgttcatcc ggcaaaccaa ccggaatcaa
    20281 accgtacata tcacgtactt cacgctcgcc ccacaccgca gccggaacgc gcggcgtcac
    20341 ggaaggatac tccggtttgt tggcatcaac ttcgacgcga accgttaccc agcacttagt
    20401 ccccttctcc atcgacagca cgtagtaaac ggcgtaatga ccattcagtt tgcgttcgtc
    20461 gttaccaaaa agcaccgaca gccagccccc ctgcttgtag tagagaaact ccaccacttc
    20521 cggcagatag ttcaccttta cggtgacagt cagctgatct ttggtctgcc aggcgtggtc
    20581 cagcacgacg cccggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc
    20641 ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat
    20701 gcaaagccaa acccagccca ggtaatgcgc ggagtaatat caagacgcag acgggccatg
    20761 ctgttttcga acagcgcgat aaccaggacg ccgaccacca gtttgacgat ggcaatcacc
    20821 aacgccagca gcagcccacc gacggtgaag gtttccattt gtccccacgg aataaacacc
    20881 ccgacgaaca tctgcagcac caccaactgt ttcaggctga taccccattt catgacgcca
    20941 aagccgctgc cgctgtactc agagagcgga ccttcctgca actcctgctc ggcttccgcc
    21001 aggtcgaacg gcagtttgcc catttcgata aaagtggcga acgcacaggc acaaagcgcc
    21061 agcaccagcg ggatgctctg gctcagcggc cagtgataaa cggtgtcggt gatgttgctg
    21121 atgttggtgg aactggcaac ctgtgcggca acccacagac caagcagcag catcggttcg
    21181 accagcacgc caagcatcgc ttcacggctc gcgccgatag cggtaaacgg actaccggta
    21241 tccagaccag aaatggcaaa gaagaaacgc gcgatggcaa agagatacag taaggtgatc
    21301 aaatcaccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtagcgata
    21361 gtcagcatga cgcccaccat cacatacggc gtcaggcgga acacccagcc ggaggcatcc
    21421 gggccaacgc tctggcgacc cagcagtttg ataatgtcgc gatactcctg caacacgccc
    21481 ggcccgcgac ggttatgcaa gcgggcgcgc gccacgcggg taataccgga gagcagcggc
    21541 gcaacggcaa atagcaccag cgcctgaatt aacggatata aaacactcat tctcaggctc
    21601 ctcgtgaaac aataatcacc accagcaccg ccagttcaac cagcgccatc cggcggaaca
    21661 gcaacgcact gccttcgcac tgccagcccg gcaccagcga caccggattc agccatttgc
    21721 gcagtttcag caccggcgca aacgcctgtt tcaccggcat ggcaaaaccg tgggcggtaa
    21781 ttaccattga tttttcatgg tcgtagccgc acacccaggc cgcgccgcgg gaacgcgatg
    21841 gcaaacgatc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca
    21901 gcagcaacgt gatcatcggt tgagaaacgg tggtgttagc aggttccagc ggcagaggta
    21961 cagcagcaga gagcatcggc agcagccacg gcgcagcaac accgccaatt acgcagcaaa
    22021 tcgccagtgc cactacgctt acgctcatca ggagcggtgc acaggtggcg ttttcggctt
    22081 ctttggtacg cggcgcgccg aggaacgtga cgccatagac tttcgccata cacatcaccg
    22141 ccagcgcacc ggtaattgcc agacccacag cgagcagcgg ccccagcaga cgggcaacaa
    22201 acgcgccact attgctcagt ttgaagaagg attgatagat aacccattcc ccggcaaaac
    22261 cgttcagcgg cggcagtgca gccattgcca tcagcccgac taacatagcg atggagataa
    22321 ccggcatttt cttgccaata ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc
    22381 agacgctccc cgccccgagg aacagtacgc ttttgaacag gctatggtta agcagatggt
    22441 acagaccacc gaccaggcca agagcaatca gcgccggttg ttcgagcgcg atacccgtta
    22501 cgccagcgcc cagccccagc aggataatgc cgatattttc cagggtgtgg taagccagta
    22561 gacgctggat attatgttcc atcaacgcat acagaccgcc gacaaacgcg gtgatcatac
    22621 cgagcaccag cagcgcgatc ccccaccaca gcggtgcatt accgcccagc agtgacaggg
    22681 ttaaaatgcc cagcaggcca attttcatga ctaccgtaga aaacaacgcg gcagctggcg
    22741 cagaggcgtt cgcatgtgcc tgcggcaccc agccgtgcag cggaataatc ccggccagca
    22801 ggccaaagcc aatcacactg agcagccaga tatcggaacc gagcggcagc tgttgcatac
    22861 gcatatccag caggcgcaga tccagcgtgc cgtaacgctg ccacagcagc cagcaagcaa
    22921 tcgccagcag cagagtgcca agacgcccca gcgcaaacca cagtttgccc tctttgctgt
    22981 tgctggtgag gaacaccgca cacaaggcca tgacttcggc cattaccacg aacatgccga
    23041 ggttgctggc aatgacggcg cagacggcgg cagccatcaa catattgatc tgcaagccgt
    23101 tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta gaggctgaca aacagaccgc
    23161 acagaccgag cgtaatcagc caaatcgcgt taagcggaga gatttgcaca tcgtagctta
    23221 ccagcgacag cgcaccgctc acgccaaccg cgccagtcag tacagtgaag cccgcggctg
    23281 ccgtatacag actaccaacc gcgccgccaa ttccggctat ccagccgctt agcgcctttt
    23341 gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa ccacgccacg ccgctattga
    23401 tcagggaaat tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc
    23461 gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg ctgacgcggg cgatatcggt
    23521 gttatccacc agatgcaggg ctttagtcgg gcacatccgc acgcaggccg gaccttgttc
    23581 atcaaagcta caaaggtcac atttgacggc gatcgcgcga atacccggca cccagtcaag
    23641 cagcgtactg atacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggcatt
    23701 tgcaggaata tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat
    23761 gccgcacagc ttgcagctta cgcacaggct ttcattcaac tgcacggccc catcaacgcg
    23821 ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca tcttcacagt ggtgacagag
    23881 ctgcggcgca gattcttttt catttagcat cactctcagg cgcggcattg attgcaggcc
    23941 gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg tggcaaccga tacagagcgt
    24001 ggagtcagca attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg
    24061 aaaacatgcc gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc
    24121 gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc cgggctttgt ccctttacca
    24181 gttggcttaa attcaccggc acattgtttt caatagcggt gtataagctg tcgtaaacgc
    24241 cagcgatttt ttcgagatag tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc
    24301 catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat
    24361 cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt
    24421 aaatacgaaa atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg
    24481 cgtgatgcag gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt
    24541 gccactggtc ctgtaggcga cgatgccgct gtgcgatgta atcggctttc tcgcttattt
    24601 cccaaatagt cattgtcagg ttacccgttt aacagagatg ccagctttaa gcattttttg
    24661 tgccaacttt taatttatta ttattaaagc gatttttaag attttggtga atttcgtcgc
    24721 cgtgtcgacg tgtcatttcg acatcatcga cattattcac tgcagggata atcaacactg
    24781 gcacaattat tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc
    24841 tctgccaacg ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg
    24901 taactggggt ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct
    24961 tttgttttga tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag
    25021 aacaagaggc cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc
    25081 gcgtccgccg ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt
    25141 tacagattcg gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg
    25201 gtgaaggcaa cctgtatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg
    25261 cgccatttgc cccggcggca cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat
    25321 ttacccccag ccagactgaa gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc
    25381 acgcaccggg catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa
    25441 ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc ctgcaagcaa ctggtgctca
    25501 acctggtttc cagcccaggt tccggtaaaa ccaccctgct gacggaaacc ttaatgcgcc
    25561 tgaaagacag cgttccgtgc gcggttattg aaggtgacca gcaaaccgtg aacgatgccg
    25621 cacgcattcg cgctaccggc acaccagcga ttcaggtgaa caccggtaaa ggctgccatc
    25681 ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc actggacgat aacggtattc
    25741 tgtttatcga aaacgttggc aacctcgtat gcccggccag cttcgatctc ggtgaaaaac
    25801 acaaagtggc ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcata
    25861 tgtttgccgc cgcctcgctg atgctgctca acaaagttga cctgttgccg tatctcaact
    25921 ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc
    25981 ttatttccgc caccagcggc gaagggatgg accagtggct gaactggctg gagacacagc
    26041 gatgtgcata ggcgttcccg gccagatccg caccattgac ggtaaccagg cgaaagtcga
    26101 cgtctgcggc attcagcgcg atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg
    26161 tcagccgcgc gtgggccagt gggtactggt acacgttggc tttgccatga gcgtaattaa
    26221 tgaagccgaa gcacgcgaca ctctcgacgc cttgcaaaac atgtttgacg ttgagccgga
    26281 tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg
    26341 ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac
    26401 accgccgaac gccctctgcg gattatggaa gtgtgcggtg gtcataccca cgccattttt
    26461 aaattcggcc tcgaccagtt actgcctgaa aacgttgagt ttatccacgg tccgggttgc
    26521 ccggtgtgcg tactgccgat gggcagaatc gacacctgcg tggagattgc cagccatccg
    26581 gaagtcatct tctgtacctt tggcgacgcc atgcgcgtgc cggggaaaca gggatcgctg
    26641 ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgtct attcgccgat ggatgcgttg
    26701 aaactggcgc aggagaatcc aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc
    26761 accatgccga ccaccgccat cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt
    26821 tacttcttct gccagcatat tacgcttatc ccgacgctgc gcagtttgct ggaacagccg
    26881 gataacggta tcgacgcgtt cctcgcgccg ggccacgtta gtatggttat cggcactgat
    26941 gcctataatt ttatcgccag cgattttcag cgtccgctgg tggtggctgg tttcgaaccc
    27001 cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga aaatagcggc ccacagcaag
    27061 gtagagaatc agtatcgtcg ggtggtaccg gatgccggta acctgctggc gcaacaggcg
    27121 attgccgatg tgttctgtgt caacggcgac agcgaatggc gcggcttagg cgtgattgaa
    27181 tcttctggcg tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc
    27241 ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt gtggcgaagt cttgacgggc
    27301 aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcgttt
    27361 ggtgcgctga tggtttcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag
    27421 gagagtgaag cgtgaataat atccaactcg cccacggtag cggcggccag gcgatgcagc
    27481 aattaatcaa cagcctgttt atggaagcct ttgccaaccc gtggctggca gagcaggaag
    27541 atcaggcacg tcttgatctg gcgcagctgg tagcggaagg cgaccgtctg gcgttctcca
    27601 ccgacagtta cgttattgac ccgctgttct tccctggcgg taatatcggc aagctggcga
    27661 tttgcggcac cgcgaatgac gttgcggtca gtggcgctat tccgcgctat ctctcctgtg
    27721 gctttatcct cgaagaagga ttgccgatgg agacactgaa agccgtagtg accagcatgg
    27781 cagaaaccgc ccgcacggca ggcattgcca tcgttactgg cgatactaaa gtggtgcagc
    27841 gcggcgcggc agataaactg tttatcaaca ccgcgggcat gggcgcaatt ccgacgaata
    27901 ttcactgggg cgcacagacg ctaaccgcag gcgatatatt gctggttagc ggtacactcg
    27961 gcgaccacgg ggcgactatc cttaacctgc gtgagcagct ggggctggat ggcgaactgg
    28021 tcagcgactg cgcggtgctg acgccgctta ttcagacgct gcgtgacatt cccggcgtga
    28081 aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag
    28141 cctgcggttg cggtattgaa atttctgaat cagcgctgcc ggttaaacct gccgtgcgcg
    28201 gcgtttgcga attgctggga ctggacgccc tgaactttgc caacgaaggc aaactggtga
    28261 tcgccgttga acgcaacgcg gcagagcaag tgctggcagc gttacattcc catccactgg
    28321 ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc
    28381 tgtatggcgt gaaacgaacc ctcgatttac cacacgccga accgcttccg cgtatatgct
    28441 aataaaattc taaatctcct atagttagtc aatgaccttt tgcaccgctt tgtggtgctt
    28501 tcctggaaga acaaaatgtc atatacaccg atgagtgatc tcggacagca agggttgttc
    28561 gacatcactc ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg
    28621 caactggtaa agcgttctgc gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag
    28681 actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca cccccattaa atatgaagac
    28741 gaaactgttc tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgttgcat
    28801 tgcagttacg aagaattttg tgaaacctgg ccgcagctgg ccgcaggtgg gctataccca
    28861 aaatttggtc actattgcct gatgccactg gcggcggaag ggcatatttt tggtggctgt
    28921 gaatttattc gttatgacga tcgcccctgg agcgaaaaag agttcaatcg tctgcaaaca
    28981 tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga gtcgcgtcgt taacaatgtc
    29041 gactatgagt tgttatgccg ggaacgcgat aacttccgca tcctggtcgc catcaccaac
    29101 gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccat
    29161 tactatttcg atattgacga tatcagtatc gtcttacgca gccaccgtaa aaacaaactc
    29221 aacatctact ccactcacta tcttgataaa cagcatcccg cccacgaaca gagcgaagtc
    29281 gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta aagagatgct gctgattaat
    29341 ctccacgagc gggatgattt agccccctat gaacgcatgt tgttcgatac ctggggcaac
    29401 cagattcaaa ccttgtgcct gttaccgctg atgtctggcg acaccatgct gggcgtgctg
    29461 aaactggcgc aatgtgaaga gaaagtgttt accactacca atctgaattt actgcgccag
    29521 attgccgaac gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aatccatcgt
    29581 ctgaaagaac ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat
    29641 agtgaatttg gcgagattat tggccgcagc gaagccatgt acagcgtgct taaacaagtt
    29701 gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg gtgaaactgg cacgggtaaa
    29761 gagctgattg cccgtgctat ccataatctc agtgggcgta ataatcgccg catggtcaaa
    29821 atgaactgcg cggcgatgcc tgccggattg ctggagagcg atctgtttgg tcatgagcgt
    29881 ggggctttta ccggtgccag cgcccagcgt atcggtcgtt ttgaactggc ggataaaagc
    29941 tccctgttcc tcgacgaagt gggcgatatg ccactggagt tacagccgaa gttgctgcgt
    30001 gtattgcagg aacaggagtt tgaacgcctc ggcagcaaca aaatcattca gacggacgtg
    30061 cgtctaatcg ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg tgagttccgt
    30121 agcgatctct attaccgcct gaacgtattc ccgattcacc tgccgccact acgcgagcgt
    30181 ccggaagata ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg
    30241 cgcaatatcg acagcattcc tgccgagacg ttgcgcacct tgagcaatat ggagtggccg
    30301 ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcgg tattgctaac acgcggcaac
    30361 gtgctgcagc tgtcattgcc agatattgct ttaccggagc ctgaaacgcc gcctgccgca
    30421 acggttgtcg ctcaggaggg cgaagatgaa tatcagttga ttgttcgcgt gctgaaagaa
    30481 actaacggcg tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg
    30541 accctgctgt cacggatgaa gcgactggga attgataaat cggcattgat ttaactgcaa
    30601 attgccggac agatctgcct gtccggcata ctattcacga ggttttttcg gacgatattt
    30661 ttccggcagt tctggcaccg gacacttgtc atcgatgaga tgacgcacgg ttaagatcgg
    30721 atgacgccac agcattctcg gcccggccca acgcataatc tgtttcatct cttcacgctt
    30781 tgcaggctgg taacagtgca ccggacactg cttacaggct ggtttctctt cgccgaacac
    30841 acatttatcc agccgctttt gcgcgtaaac aaacaacgcc tcgtaatgct ccggctccgc
    30901 tgacgcctgc gggcatttcg cttgataaag atcgatcatt tttttaatcg tcagtttttc
    30961 acgagagata cgcttgccgg acatactgcc tccacctcat taagatgcat ttatattaca
    31021 acttaatctt aaagggcact atgactctaa agaagaaggg ttagccaacc gatacaattt
    31081 tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa cagcatcttt cccggaacca
    31141 gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat taactccggt atcatgtgcg
    31201 ccttatgtga ttacaacgaa aataaaaacc atcacacccc atttaatatc agggaaccgg
    31261 acataacccc atgagtgcaa tagaaaattt cgacgcccat acgcccatga tgcagcagta
    31321 tctcaagctg aaagcccagc atcccgagat cctgctgttt taccggatgg gtgattttta
    31381 tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg ctggatattt cactgaccaa
    31441 acgcggtgct tcggcgggag agccgatccc gatggcgggg attccctacc atgcggtgga
    31501 aaactacctc gccaaactgg tgaatcaggg cgagtccgtt gccatctgcg aacaaattgg
    31561 cgatccggcg accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg ttacgccagg
    31621 caccatcagc gatgaagccc tgttgcagga gcgtcaggac aacctgctgg cggctatctg
    31681 gcaggacagc aaaggtttcg gctacgcgac gctggatatc agttccggtc gttttcgcct
    31741 gagcgaaccg gctgaccggg aaacgatggc ggcagaactg caacgcacta atcctgcgga
    31801 actgctgtat gcagaagatt ttgctgaaat gtcgttaatt gaaggccgtc gcggcctgcg
    31861 ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga atctgcaatt
    31921 tgggacccgc gatctggtcg gttttggcgt cgagaacgcg ccgcgcggac tttgtgctgc
    31981 cggttgtctg ttgcagtatg cgaaagatac ccaacgtacg actctgccgc atattcgttc
    32041 catcaccatg gaacgtgagc aggacagcat cattatggat gccgcgacgc gtcgtaatct
    32101 ggaaatcacc cagaacctgg cgggtggtgc ggaaaatacg ctggcttctg tgctcgactg
    32161 caccgtcacg ccgatgggca gccgtatgct gaaacgctgg ctgcatatgc cagtgcgcga
    32221 tacccgcgtg ttgcttgagc gccagcaaac tattggcgca ttgcaggatt tcaccgccga
    32281 gttgcagccg gtactacgtc aggtcggcga cctggaacgt attctggcgc gtctggcgtt
    32341 gcgtaccgct cgcccacgcg atctggcccg tatgcgtcac gctttccagc aactgccgga
    32401 gctgcgtgcg cagttagaaa ctgttgatag tgcaccagta caggcgctac gtgagaagat
    32461 gggcgagttt gccgagctgc gcgatctgct ggagcgagca atcatcgaca caccgccggt
    32521 gctggtacgc gacggtggtg ttatcgcatc aggctataac gaagagctgg atgagtggcg
    32581 cgcgctggct gacggcgcga ccgattatct ggagcgtctg gaagtccgcg agcgtgaacg
    32641 taccggcctg gacacgctaa aagttggctt taatgcggtg cacggctact acattcaaat
    32701 cagccgtggg caaagccatc tggcacctat caactatatg cgtcgccaga cgctgaaaaa
    32761 cgccgagcgc tacatcattc cagagctaaa agagtacgaa gataaagtcc tcacttcaaa
    32821 aggcaaagca ctggctctgg aaaaacagct ttatgaagag ctgttcgacc tgctgttgcc
    32881 gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg tgctggtgaa
    32941 cctggcggaa cgggcctata ccctgaacta cacctgcccg accttcattg ataaaccggg
    33001 cattcgcatt accgaaggcc gccatccggt ggttgaacag gtgctgaacg agccatttat
    33061 cgccaacccg ctgaatctgt caccgcagcg ccggatgttg attattaccg gtccgaacat
    33121 gggcggtaaa agtacctata tgcgccagac cgcactgatt gcgctgatgg cctatatcgg
    33181 cagctacgta ccggcgcaaa aagtcgagat tggcccgatt gaccgtatct ttacccgcgt
    33241 aggggcagcg gatgatctgg cttccgggcg ttcaaccttt atggtggaga tgaccgaaac
    33301 cgctaatatt ctgcataacg ccaccgagta cagtctggtg ctgatggatg agattgggcg
    33361 cggaacgtcc acttacgatg gtctgtcgct ggcgtgggcg tgcgcggaaa atctggcgaa
    33421 taagattaag gcgttgacgc tgtttgccac ccactatttc gagctgaccc agttaccgga
    33481 gaaaatggaa ggcgtcgcca acgtgcatct cgatgcactg gagcacggcg acaccattgc
    33541 ctttatgcat agcgtgcagg atggcgcggc gagcaaaagc tacggcctgg cggttgcagc
    33601 tctggccggc gtgccaaaag aggttattaa gcgcgcacgg caaaaactgc gtgagctgga
    33661 aagcatttcg ccgaacgccg ccgctacgca agtggatggt acgcaaatgt ctttgctgtc
    33721 cgtaccggaa gaaacctcgc ctgcagtcga ggcactggaa aacctcgatc cggattcact
    33781 caccccgcgt caggcgctgg aatggattta tcgcctgaag agtctggtgt aataataatt
    33841 cccgatagtc ttttgctatc gggaatatta acgataactg acgaatcaaa taaaaatacc
    33901 ctgtataata ggaaagctta ttttacaggg taaaaccatg ccatctacac gctatcaaaa
    33961 aatcaatacc catcactatc gccatatatg ggtcgttggt gatattcatg gtgaatatca
    34021 gttattacaa tcccgcttac atcaactctc ttttttcccc gaaaccgact tacttatttc
    34081 tgtcggcgat aatattgatc gtgggccgga gagtcttaac gtcctgcgcc tgctaaacca
    34141 gccctggttt atctccgtta aaggcaacca cgaagcaatg gcgctggatg cgttcgcgac
    34201 cggcgatggc aatatgtggc ttgccagcgg tggtgactgg tttttcgatt taaatgattc
    34261 agagcaaaaa gaagctacag atctgttgct gaaattccat caccttccac atattattga
    34321 aatcactaac gacaacataa aatatgtcat cgcacatgca gattatccgg ggagtgaata
    34381 tctctttggt aaagaaatag cggagagcga attactctgg cctgttgatc gtgtgcagaa
    34441 atcgcttaat ggcgagttac aacaaataaa cggcgctgat tattttatat ttggacatat
    34501 gatgtttgat aacattcaga cgttcgctaa ccagatttat attgataccg gatcgccgaa
    34561 aagcgggcgg ctgtcatttt ataaaataaa gtaatgaggc cgggtaaagc aaagccacta
    34621 cccggcactt tttattagcg cttaccttcc gccagcagcg gcgggatgct cggcaccatt
    34681 ggcgcgtcat caatatcttt ctgcgtcatg cggaacgctt cgggataatg ttcgcggctg
    34741 gtacgacgca gcggttcggt atcgcgccag gtataaaggc aatgctggca ctgatatacc
    34801 gtccagacat ctttcaccgg cgatttcgcc atcacttcaa tctgttcatc ggcacaacgt
    34861 ggacaaatca tcttgttctc cttatttacg tgcggccagc atagcggtca gtttttcagc
    34921 ccaggctttg gtttccggca aatccaccac cggctggctg tagtgaccac ggttgtccgg
    34981 ggcgacaggc gtggtggcgt cgataatcag cttgtcggtg atccccgccg ggcttgagcc
    35041 agggtcgagt tccagcacgg acatattcgg caactgcacc aaatcccctg ccggatttac
    35101 tttcgaggag agcgcccaca tcacctgcgg caggttgaac gggtcaacgt cttcatcgac
    35161 cataatcacc atctttacgt agcccagacc gtgcggcgtg gtcatcgcac gcaggcccac
    35221 cgcgcgggca aagccgccgt agcgtttttt ggtggagata atcgccagca ggccgtgggt
    35281 gtacatggcg tttaccgcct gcacttccgg gaactcggct ttcagttgtt gatacagcgg
    35341 cacacaggtg gcaggcccca tcaggtagtc gatttcggtc cacggcatgc cgaggtacag
    35401 cgattcgaaa atcggcctgg tgcggtaaga gactttatcg atacgcacca cggtcatgtt
    35461 acgcccgccg gagtagtgcc cggtaaactc accgaacggc ccttcgattt cgcgtttgcg
    35521 gctttcgata accccttcga ggatcacttc tgaaccccac ggcacatcaa aaccagtcaa
    35581 tggcgcggtg gcgatcgggt acgggctttc gcgcagcgcg cctgccattt catactcaga
    35641 ctgatcgtat ttcagcggcg tggcgcccat aagggtgatg atcggatcgt tacccagggt
    35701 gatggcaatc ggcagatctt caccgcgctc ttccgcttta tgcagatgca gggcgatatc
    35761 gtgcatcggc accggttgca ggccgagctt acgcttgccc ttcacttcca tgcggtagat
    35821 accgacgttc tgcttgccga agttatccgg gtcgagcgga tcgcgggaaa ccacgcacgc
    35881 tttgtcgaga tagaaaccgc catcaccgtc gtttaaacga aacagcggca ggatatcgaa
    35941 cagattaatc tcgtcaccat cgacggtgtt ctgcgcccag gctggattgg cgcggcgctc
    36001 cggggcgatc gggaagttat cccagcggcg gataaactca tcaatctgtt ttttaaccgg
    36061 ggtgtttggc ggcaggccca aggaaatcgc gtggttctgc caggaaccga tggtattcat
    36121 cgccacgcgg gcatcggtaa aaccgcgaat attatcaaac cacagcgccg gtgcaccatc
    36181 gccgatacgc ccggtggcgt tggcagcggc agccagatcc ggttccgcgt tcacctcttc
    36241 gctgattttc agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc gtaaatcatc
    36301 aaatgccatt attcattctc ctgagaaaaa ttccgggcct gcggcaatcc ttgccagcgc
    36361 ctggcgtagg gatgttcaag gccaaattga tccagcacgc gggctaccac gtggtggaca
    36421 atgtcatcta ccgtttcggg atggttataa aaggcaggca tcggcggcac catcgccacg
    36481 cccatgcgtg aaagtgcgag catattttcg agatggatgg tgctaagcgg catttcacgc
    36541 ggcaccagca ccagtttgcg gccttcttta agcacgacgt ccgccgcgcg ccctaccagg
    36601 ccatcagcgt aaccagcgcg gataccggcg agcgttttca tactgcacgg aataacgatc
    36661 atgccgtctg tacgaaagga acctgaggag atggtcgccg cctgatccgc cgggttatgg
    36721 ctgaagtcag cgagggcagc aacatcgcgg gcgctgtaag gcgtttccag ttcaatggtg
    36781 gttttcgccc acttcgacat caccagatga gtctcgacat tcggcatctc ccgcagcgct
    36841 tgcagtaatg ccacaccaag aggcgcaccg gtagcccctg tcatcccgac gatcagtttc
    36901 attgcttact ctcttaattg ttcgtatacg aacattatta ttaaaatacg cctctgttct
    36961 cattgcggca agcactgaaa agtaacaaca tccccttcgt cactgaaaaa ccgctatgat
    37021 agcggcagat agtttgcccg gagtttacat gacgttacga aataaagcgt tccatcagtt
    37081 acgacagctt tttcagcagc acacggctcg ctggcagcac gagttacctg acctgaccaa
    37141 accacagtat gcggtgatgc gcgccattgc tgataagcct ggtatcgaac aggtggcact
    37201 gatagaagca gcagtcagca ccaaagcaac gttggcagaa atgttggcaa gaatggagaa
    37261 tcgtggctta gtcagacgtg aacatgatgc cgctgacaag cggcgtcgct ttgtctggct
    37321 gactgccgaa ggagaaaaga tacttgctgc ggcgataccg attggtgaca gcgtggatga
    37381 ggaatttttg gggcgtttga gtgcagaaga gcaggagctg tttgtgcagc tggtgcgcaa
    37441 gatgatgaac acataggatg caaattgccg ggtaggacgc tgacgtgtct tatccaggcg
    37501 acaaaaacac aagcttccca aacaaaagaa aaaggccagc ctcgcttgag actggccttt
    37561 ctgacagatg cttacttact cgcggaacag cgcttcgata ttcagcccct gcgtttgcag
    37621 gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag
    37681 gccaatttca cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg
    37741 acgtgccagt acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg
    37801 cttcatatcg tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat
    37861 gtccagcaac gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc
    37921 gttaagacga agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc
    37981 ttccgcactt ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac
    38041 gttcagctcc tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc
    38101 ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc
    38161 cgggtcaaac ttctctaccg cacggatcag ccccaggttg ccctcttcga taaggtccag
    38221 caacgccaga ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact
    38281 ctcgatcatc cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac
    38341 ttcttcttcg gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc
    38401 gtccaacaca cgctatgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc
    38461 actgggttcc tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa
    38521 ttccgcatct tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct
    38581 acccgtgatc ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc
    38641 ggcaaataac gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt
    38701 gttgaactgg ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt
    38761 tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt
    38821 ttgatgataa tcagattacc gtagccgcgc agcgcgttac cggcataaac aacacggcca
    38881 tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc
    38941 ccctcagaag cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag
    39001 ataggcgtac tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc tgttggtacc
    39061 gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat tttgttagca
    39121 ctctgttcac ccgaagactc agaatacgta attgtcggtt gcgacgcaac agcaacggtg
    39181 gaattttgtg caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatggca
    39241 tttccgccag tgattggcgt accggaagca ttacccacct gtaaggtctg accgacgttc
    39301 agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta
    39361 atccaggcga tatagaaaag cgtgtcgcct tttttcacgg tataggtact gccgctataa
    39421 ctgcctttcg gaatgttccc atactgacgg ttatagacga tgcgtccgtt ttccatctgt
    39481 accggctgct gagctactgg ctgcactggc tggatttgcg gttgctgcgc tgtagacgtc
    39541 gtccccattt tcggcggcgg cgtaatcaac ataccagaat tggtatgtgc aggcgcattg
    39601 ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat
    39661 agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc
    39721 atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcacca
    39781 tactgaacac tggaaaagat gttcacgata cgctgacctg cggcaaaata accaggaaaa
    39841 atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac
    39901 ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg
    39961 gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagcgc
    40021 agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg
    40081 ccaaccttgc catccatcgc catgacgggt tgaaacatta tgtaaatcaa gatttttcag
    40141 gcggcgacgc gcctgccact gcaagccttt aatccgttca accgagcaaa catgctggac
    40201 aagatgcgcc aggattgccg tttgatatcc cgaaccggtg ccaatttcca gcacccgcga
    40261 ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaatcgt
    40321 ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc
    40381 aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc
    40441 ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact
    40501 cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca
    40561 tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg
    40621 gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc
    40681 tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg
    40741 atttgatcca agggtaaatc cggaacatta atattaagaa tacgcccggt gcgcagcggc
    40801 tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta
    40861 tgcccgtcaa gcgagacggc aagcgccgga aaacctaaat gacggccttc catcgcggcg
    40921 gctaccgtac cggaataaat aacatcatcc cccagattcg gcccggcgtt aattccggac
    40981 acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac gcaatcggtc
    41041 ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat
    41101 tccagtgtca gagaatttga agcgccgctg cggttacgat cgggggcgac cacctgaacg
    41161 tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca
    41221 tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct
    41281 ggttgcaaaa ctacccgccg gaagccagaa acggatttct acggtgacgt catcccacca
    41341 attccagctt aattgttgcg gatacagcag catcgctctg cgcgcggctt caactttttc
    41401 gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcctgtt cgaatgccag
    41461 cgcttcacgc tgagttcccc attcaccact gcccggcaat gcggcggtta tcatcagctc
    41521 tttatcgttg acgcgacgct gtaattccac cagttcttcg gtggttgcaa caaaccagct
    41581 accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt
    41641 gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact
    41701 ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa
    41761 gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac cttttacgca
    41821 aatatcgatc agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt
    41881 aaaggcgtta cctttcagcg cccctaaacg cagcttgcgc ttgtgccgcg catactccag
    41941 cacctggcag ccttccaatt gaaaggcgct cagatcgggc atttccttgc ccggcacgcg
    42001 agcgcataac cactgttctg taacagcatg tttgtctttt tgcccagcaa agctgacttc
    42061 acgggcatga attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc
    42121 gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc
    42181 ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt gcggtttacc
    42241 gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc
    42301 gcttcacagg caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct
    42361 ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt
    42421 ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgccaagg
    42481 gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctatcggca
    42541 cctttaaatg ccggatcggt atccgggaac agtttgccga tatcccccag cgccgccgcg
    42601 ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc catcagaatg cgccagcaat
    42661 cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggcg
    42721 tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga
    42781 ggtaaaactc ggccagtgcc aaatcctccg ggcgcgtgac tttaatgtta tccgcacggc
    42841 cttcgaccaa ctgaggatgg aatccgcaat attccagcgc cgaggcttcg tcggtaatag
    42901 ccgcgccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga gggaaaaatt
    42961 gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc
    43021 ccggttcggc acgtttcata gtatcgcgca ctggtgcggc taggatccct cccgtgcggc
    43081 tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg
    43141 cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt caaacctgcc agcacggaat
    43201 cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt
    43261 gtgcaaaacg gctatcgcca ggacttatgg caatgacgac acgtttcacc cggggatgcg
    43321 ccagcagcgc atacaccgag tgttcaagaa tggtttgatt accgattgag agatattgct
    43381 taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat
    43441 ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag
    43501 actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca
    43561 tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa
    43621 tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg
    43681 ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat
    43741 actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg
    43801 aaaaacggca tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac
    43861 cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca
    43921 gaacataatc tcattattag ttacggtttg aattatgaac agaggagaca agaaagtaca
    43981 aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt catcagggtg
    44041 aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact
    44101 accgcaacgg gcatcagcgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg
    44161 ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat aaaaccgacc
    44221 acggcaccag gcagtgacca tgtggtttct tcatcctcag taaggctgtc gttatttgtt
    44281 agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcatgg
    44341 aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg
    44401 ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg attcaggcgc
    44461 ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata
    44521 taagcctttt ggatcgcggg cttcgcaaat cgccagtggc gtatcgacaa acacttcgat
    44581 aaagcgccct tctcctacgc gttcgcgaac catctggcgt tcggcgcggt gtggcgagat
    44641 aaatgcggtc agcaccacca gtccggcttc aaccatcaaa ttcgccactt caccgacgcg
    44701 acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg
    44761 aacattgtcg ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag
    44821 cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc
    44881 acgatgaccg tggtgtagct cgcgttgttg cacagtgacc ggatggctat gccagacgac
    44941 gttttcgtca tgcagcgcca ttatttctcc cccagcaaat cgcgcgcgcc ccagtgtgga
    45001 aagtggcggc gaaccagggc attcaattcc agttcgaatg cactaaattc agatggcgca
    45061 gcagttgcct ggctaactgg ctcatgcacc ataccggcac ctacggtcac attgctcagg
    45121 cgatcgataa aaatcagccc accggtaacc gggttttgct gataacgatc taacaccagt
    45181 ggctcgtcaa aagtgagatc caccaggccg atgccgttca gcggcaggtt ttcaacttcg
    45241 cgttgggtaa ggttattaat atcaacctga taacgaatgc catcaacacg agaacgcgtc
    45301 ttcttaccgg caattttgat gtcgtaactc tggcccgggg aaagcggctg ttccgccatc
    45361 cataccacat ccaccgacgc gctctgcaca gctggtaacg cttcgtctgc cgccagcagc
    45421 agatcgccac ggctgatgtc gatctcatcc gtcagcacca gggtgatagc ttctccggca
    45481 aaggcttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga ttccacacca
    45541 gagggcagca cttttacacg ttgcccgact tccacgcgac cggatgccag cgttccggcg
    45601 tagccacgaa aatcgagatt tgggcggtta acgtactgca ccgggaagcg cattggctgg
    45661 gcatccacca ctcgctgaat ctccacggtt tccagcactt cgagcagtgt cggaccgctg
    45721 taccacggca tactttcact ttgcgaagcc acgttgtcac cttccagtgc ggagagcggc
    45781 acaaagcgga tatccagatt acccggcagc tgcccggcaa aggtcagata atcttcacga
    45841 atacgggtga acgtctcttc actgtaatcc accagatcca ttttgttgat cgccacgacc
    45901 agatgtttga tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg
    45961 cctttacggg catcgatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata
    46021 ttgcgggtgt actgctcgtg ccctggagtg tcggcgataa taaatttacg cttctcggta
    46081 gagaaatagc ggtaggccac gtcaatggtg atgccctgtt cacgctcagc ttgcaggccg
    46141 tccaccagca gagccagatc cagcttttcg ccctgggtgc cgtgacgctt actgtcatta
    46201 tgcagcgatg agagctgatc ttcatagatt tggcgggtat cgtgcaacag acgaccaatc
    46261 agggtacttt tgccgtcatc gacgctacca caggtcagaa aacgcagcag gcttttatgt
    46321 tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg
    46381 gtgttcatct taaaaatacc cctgacgttt tttcagctcc atagaaccag cttggtcgcg
    46441 gtcaatcacg cgcccctgac gttcactggt ggtggaaacc agcatctctt cgatgatctc
    46501 cggcagcgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg
    46561 gaaacgcacc atccgttttt taatcacttc gcccggttgc aggtcgatac ggttgtcatc
    46621 aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga gatacagcgg
    46681 cacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga
    46741 gagcgggaaa acgcggatgc tttcgccttt gttaatctgc ccgttatagt tgtgccacag
    46801 ctccgggcgc tgatttttcg ggtcccagcg atggaagcga tcacggaaag agtagatacg
    46861 ctctttagca cgggatttct cttcgtcgcg gcgcgcacca ccgaaggcgg catcaaaacc
    46921 gtatttattc agcgcctgct tcaggccttc ggtcttcata atatcggtat gtttcgcgct
    46981 gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggttttgat gcaccagcag
    47041 ctcgcagccg taggctttcg ccgtacgatc gcggaactca tacatctcac ggaatttcca
    47101 gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc
    47161 cagatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa
    47221 ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt
    47281 aagtcgtatt tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg
    47341 tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct
    47401 taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg
    47461 gcgtttagga agatttgaaa ttgttttagc gcagcggcag tttcatacta tggcggtaaa
    47521 aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg
    47581 ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg
    47641 attttgccaa tacacaggcg cgacatattg ccactttctt tccgggacga atgaccggaa
    47701 cacccgcaga aatgttatct gccgattata ttcggcaaca gtttcagcaa atgggttacc
    47761 gcagtgatat tcgtacgttt aatagccgat atatttatac cgcccgcgat aaccgcaaaa
    47821 actggcacaa cgtgacggga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc
    47881 agatcattat tatggcgcat ctggatacct atgccccgca gagcgacgca gatgcagatg
    47941 ccaatctcgg cgggctgacg ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca
    48001 tgctggaact ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg
    48061 cgaccagtgg agaagaggaa gggaaattag gcgctgagaa tttactcaag cggatgagtg
    48121 acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata
    48181 aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca
    48241 gggcgctggc aattgcgcgt agtcatggaa ttgccgcaac gaccaatccg ggtttgaata
    48301 aaaattatcc gaaaggcact ggatgttgta atgacgcaga aatattcgac aaagcgggca
    48361 ttgctgtact ttcggtggaa gcgacaaact ggaatcttgg gaataaagat ggttatcagc
    48421 aacgcgcaaa aacagccgca ttccctgcgg gaaatagctg gcatgacgta agactggata
    48481 atcagcaaca tattgataaa gcacttcctg gaagaataga acgtcgctgc cgtgacgtta
    48541 tgcggataat gctaccgctg gtgaaggagt tggcgaaggc gtcttgatgg gttggaaaat
    48601 gggagctggg tgttctaccg caggggcggg gaattctaag tgatatccat catcgcatcc
    48661 agtgcgcccg gtttatcccc gctgatgcgg ggaacacagc ggcacgctgg attgaacaaa
    48721 tccctgggcc ggtttatccc cgctggcgcg gggaacacaa accgaaacac acgatcaatc
    48781 cgaatatgag cggtttatcc ccgctggcgc ggggaacact ttggtgacag tttttgtcac
    48841 tgttttggtg acg
//
LOCUS       DBFIFS010000009        45962 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.009, whole genome shotgun sequence.
ACCESSION   DBFIFS010000009 DBFIFS010000000
VERSION     DBFIFS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.009"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(363..1763)
                     /gene="asnS"
                     /locus_tag="N7A07_000522"
     CDS             complement(363..1763)
                     /gene="asnS"
                     /locus_tag="N7A07_000522"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU2475255.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1932..3134)
                     /gene="pncB"
                     /locus_tag="N7A07_000523"
     CDS             complement(1932..3134)
                     /gene="pncB"
                     /locus_tag="N7A07_000523"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU2475256.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3400..6012
                     /gene="pepN"
                     /locus_tag="N7A07_000524"
     CDS             3400..6012
                     /gene="pepN"
                     /locus_tag="N7A07_000524"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU2475257.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6055..6822)
                     /gene="ssuB"
                     /locus_tag="N7A07_000525"
     CDS             complement(6055..6822)
                     /gene="ssuB"
                     /locus_tag="N7A07_000525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU2475258.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6819..7610)
                     /gene="ssuC"
                     /locus_tag="N7A07_000526"
     CDS             complement(6819..7610)
                     /gene="ssuC"
                     /locus_tag="N7A07_000526"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU2475259.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7622..8767)
                     /gene="ssuD"
                     /locus_tag="N7A07_000527"
     CDS             complement(7622..8767)
                     /gene="ssuD"
                     /locus_tag="N7A07_000527"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU2475260.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            complement(8764..9723)
                     /gene="ssuA"
                     /locus_tag="N7A07_000528"
     CDS             complement(8764..9723)
                     /gene="ssuA"
                     /locus_tag="N7A07_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU2475261.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9716..10291)
                     /gene="ssuE"
                     /locus_tag="N7A07_000529"
     CDS             complement(9716..10291)
                     /gene="ssuE"
                     /locus_tag="N7A07_000529"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU2475262.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            10647..11186
                     /gene="elfA"
                     /locus_tag="N7A07_000530"
     CDS             10647..11186
                     /gene="elfA"
                     /locus_tag="N7A07_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2475263.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            11269..11970
                     /gene="elfD"
                     /locus_tag="N7A07_000531"
     CDS             11269..11970
                     /gene="elfD"
                     /locus_tag="N7A07_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2475264.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            11995..14594
                     /gene="elfC"
                     /locus_tag="N7A07_000532"
                     /pseudo
     CDS             11995..14594
                     /gene="elfC"
                     /locus_tag="N7A07_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            14585..15655
                     /gene="elfG"
                     /locus_tag="N7A07_000533"
                     /pseudo
     CDS             14585..15655
                     /gene="elfG"
                     /locus_tag="N7A07_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            15683..16210
                     /gene="ycbU"
                     /locus_tag="N7A07_000534"
     CDS             15683..16210
                     /gene="ycbU"
                     /locus_tag="N7A07_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2475265.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            16218..16733
                     /gene="ycbV"
                     /locus_tag="N7A07_000535"
     CDS             16218..16733
                     /gene="ycbV"
                     /locus_tag="N7A07_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2475266.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            16699..17435
                     /gene="ycbF"
                     /locus_tag="N7A07_000536"
                     /pseudo
     CDS             16699..17435
                     /gene="ycbF"
                     /locus_tag="N7A07_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            17546..18556
                     /gene="pyrD"
                     /locus_tag="N7A07_000537"
     CDS             17546..18556
                     /gene="pyrD"
                     /locus_tag="N7A07_000537"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU2475267.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            18730..19272
                     /gene="zapC"
                     /locus_tag="N7A07_000538"
     CDS             18730..19272
                     /gene="zapC"
                     /locus_tag="N7A07_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU2475268.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(19269..20378)
                     /gene="ycbX"
                     /locus_tag="N7A07_000539"
     CDS             complement(19269..20378)
                     /gene="ycbX"
                     /locus_tag="N7A07_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU2475269.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            20622..22730
                     /gene="rlmKL"
                     /locus_tag="N7A07_000540"
     CDS             20622..22730
                     /gene="rlmKL"
                     /locus_tag="N7A07_000540"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU2475270.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            22742..24649
                     /gene="uup"
                     /locus_tag="N7A07_000541"
     CDS             22742..24649
                     /gene="uup"
                     /locus_tag="N7A07_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2475271.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            24779..26032
                     /gene="pqiA"
                     /locus_tag="N7A07_000542"
     CDS             24779..26032
                     /gene="pqiA"
                     /locus_tag="N7A07_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU2475272.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            26037..27677
                     /gene="pqiB"
                     /locus_tag="N7A07_000543"
     CDS             26037..27677
                     /gene="pqiB"
                     /locus_tag="N7A07_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU2475273.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            27674..28237
                     /gene="pqiC"
                     /locus_tag="N7A07_000544"
     CDS             27674..28237
                     /gene="pqiC"
                     /locus_tag="N7A07_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU2475274.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            28493..28660
                     /gene="rmf"
                     /locus_tag="N7A07_000545"
     CDS             28493..28660
                     /gene="rmf"
                     /locus_tag="N7A07_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU2475275.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(28730..29248)
                     /gene="fabA"
                     /locus_tag="N7A07_000546"
     CDS             complement(28730..29248)
                     /gene="fabA"
                     /locus_tag="N7A07_000546"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU2475276.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(29317..31077)
                     /gene="ycbZ"
                     /locus_tag="N7A07_000547"
     CDS             complement(29317..31077)
                     /gene="ycbZ"
                     /locus_tag="N7A07_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU2475277.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            31263..31715
                     /gene="matP"
                     /locus_tag="N7A07_000548"
     CDS             31263..31715
                     /gene="matP"
                     /locus_tag="N7A07_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU2475278.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(31790..32830)
                     /gene="ompA"
                     /locus_tag="N7A07_000549"
     CDS             complement(31790..32830)
                     /gene="ompA"
                     /locus_tag="N7A07_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU2475279.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(33187..33696)
                     /gene="sulA"
                     /locus_tag="N7A07_000550"
     CDS             complement(33187..33696)
                     /gene="sulA"
                     /locus_tag="N7A07_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU2475280.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            33915..34544
                     /gene="sxy"
                     /locus_tag="N7A07_000551"
     CDS             33915..34544
                     /gene="sxy"
                     /locus_tag="N7A07_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU2475281.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(34507..36669)
                     /gene="yccS"
                     /locus_tag="N7A07_000552"
     CDS             complement(34507..36669)
                     /gene="yccS"
                     /locus_tag="N7A07_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU2475282.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(36679..37125)
                     /gene="yccF"
                     /locus_tag="N7A07_000553"
     CDS             complement(36679..37125)
                     /gene="yccF"
                     /locus_tag="N7A07_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU2475283.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            37248..39302
                     /gene="helD"
                     /locus_tag="N7A07_000554"
     CDS             37248..39302
                     /gene="helD"
                     /locus_tag="N7A07_000554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU2475284.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(39334..39792)
                     /gene="mgsA"
                     /locus_tag="N7A07_000555"
     CDS             complement(39334..39792)
                     /gene="mgsA"
                     /locus_tag="N7A07_000555"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU2475285.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(39888..40550)
                     /gene="yccT"
                     /locus_tag="N7A07_000556"
     CDS             complement(39888..40550)
                     /gene="yccT"
                     /locus_tag="N7A07_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU2475286.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            40723..41136
                     /gene="yccU"
                     /locus_tag="N7A07_000557"
     CDS             40723..41136
                     /gene="yccU"
                     /locus_tag="N7A07_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU2475287.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(41181..41498)
                     /gene="hspQ"
                     /locus_tag="N7A07_000558"
     CDS             complement(41181..41498)
                     /gene="hspQ"
                     /locus_tag="N7A07_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU2475288.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(41556..42746)
                     /gene="rlmI"
                     /locus_tag="N7A07_000559"
     CDS             complement(41556..42746)
                     /gene="rlmI"
                     /locus_tag="N7A07_000559"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU2475289.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            42841..43119
                     /gene="yccX"
                     /locus_tag="N7A07_000560"
     CDS             42841..43119
                     /gene="yccX"
                     /locus_tag="N7A07_000560"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU2475290.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(43116..43445)
                     /gene="tusE"
                     /locus_tag="N7A07_000561"
     CDS             complement(43116..43445)
                     /gene="tusE"
                     /locus_tag="N7A07_000561"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU2475291.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(43536..44195)
                     /gene="yccA"
                     /locus_tag="N7A07_000562"
     CDS             complement(43536..44195)
                     /gene="yccA"
                     /locus_tag="N7A07_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU2475292.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(44603..45609)
                     /locus_tag="N7A07_000563"
                     /pseudo
     CDS             complement(44603..45609)
                     /locus_tag="N7A07_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(45587..45829)
                     /locus_tag="N7A07_000564"
     CDS             complement(45587..45829)
                     /locus_tag="N7A07_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU2475293.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT        11779 a        11230 c        11841 g        11112 t
ORIGIN      
        1 ttccatagag gcgtgatgat atttacagca atttttatct atttatatga tttccttata
       61 tttaaattaa ctaaacggaa attttgtttc tgatggaaac tttatcgacc tggcccaaaa
      121 tcctctttca gacatccaga atgccaaaaa aatagtatga aaattgtgct atcagctaag
      181 aaaaaactaa tccgcaataa gaatgcgggt tattttttgt gcgtaaatgt tgcaatgtaa
      241 tattccgtaa caggatgatc gttatggcgg ggttgcaaat agattgattg acttaacaaa
      301 cagataactt gacagaaagg ataaaaaaga gccagcgggt gctggctctt gtagacgcta
      361 aattagaagc tggcgttacg cggagttcgt gggaacggaa tcacatcacg cacgttttgc
      421 acaccagtta cgtaagcaat caggcgttca aaaccaagac cgaaaccgga atgcggaaca
      481 gtaccgtagc gacgcagatc gcgataccac cagtaatctt ctttgttcag gcccatttcc
      541 agcatacgct cgtccagcac gtccagacgc tcttcacgct gggagccacc aatgatctca
      601 ccgatgcccg gagccagaac atccatagcc gcaacggttt taccgtcttc gttgaggcgc
      661 atatagaacg ctttaatatc tttcggatag tttttaacca ctaccggtgc tttaaagtgt
      721 tcttccgcca gataacgctc atgctcagaa gagagatcga cgccccagta aaccgggttt
      781 tcaaacttcc tgccgcagtt ttcgagaatg gttactgctt cggtgtaatc cacctgcgcg
      841 aaatcggcct caataaagcg ttccagacgt gaaacggcat ctttatctac gcgttcagcg
      901 aagaatttca tgtcgtcagc gcgttcttcg agaaccgctt tgaagacata tttcagcatg
      961 gcttcagcca gacccgcgat atcgttcagg ttagcaaacg ccacttccgg ctccagcatc
     1021 cagaattccg ccaggtgacg gctggtgttg gagttttcag cacggaaagt cgggccgaag
     1081 gtataaattt tggataatgc gcaagcgtag gtttcgccgt tcaactggcc agatacggtc
     1141 aggaaagact ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg atcgttacgc
     1201 ggcagatttt ccagatccag cgtagaaacg cggaacattt cgccagcccc ttcggtatca
     1261 gacgcagtaa tcagtggcgt tgaaacccag aagaatccct gctcgttaaa gaagcgatgc
     1321 agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga
     1381 cgcaggtgag cgacttcacg cagatactca atgctgtggc gttttgccgc catcgggtaa
     1441 gtgtctggat cttcaaccca accagcaact tcaaccttgc tggtctgaat ttcaaattgt
     1501 tgcccctggc ccggcgacgc cacgacttta cccgtcacaa tgaccgagca gccggtggtc
     1561 agacgcagga cgtcttcatt gtaattgggc agagaattat tgatgacagc ctgtacagga
     1621 tcaaagcagg aaccgtcata aacggcgagg aaggagatgc cagcttttga atctcggcgg
     1681 gtacgtaccc atccgcgtac ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg
     1741 tcggctacag gcacaacgct cataatattc tctctgttaa tagtcggaaa aaataaacac
     1801 ttgtccaccc gtaaatgggg ggatacctat gttacctggc atctgcaatc agacaagcag
     1861 aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa aaaaagggag ccgattagct
     1921 ccctgatgat attaactggc ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc
     1981 cgaacaaacg ctttatcatg gcagatagtt ttgccagggc tgtcagaaag tttcgccacc
     2041 ggtttaccgt tacactctac caacttaatc acaatattca ggggttttac ctgggggata
     2101 tcgcaggtca ggcgagtccc aataccaaaa cttaattgca cgcgggaaga gaagtggcgg
     2161 tatagctcaa ccgctttgcg taaatccaga ttgtcagaga aaaccagcgt tttactctgt
     2221 ggatcaattt ccagcttttc ataatgtgca atggcttttt caccccattc aaccgggtcg
     2281 ccagagtcat gacgcaggcc ctgataccga ctagcgaact cgacaccgaa atcacgcagg
     2341 aaagcatcca tagtgatgca gtcggttaat gcaatgccaa gttggtcggg atactcttcc
     2401 agccaggcag caagtgcagc tcgctggctg ttggctagat ccgggctgat ttgctgatgt
     2461 gcctggaacc attcgtgtgc ctgtgttccc atcggcgtga gggaaagccg acgcgccaga
     2521 tcgtagttgc tggtgcccac gaaccaggat tcctgttgca gacgcttaac gatggtttct
     2581 tgtacttcgc gagaaaaacg gcgacgggtg ccaaaatcca tcagatggaa gcgcgacata
     2641 tcaagaccag cggttaacgc cgagaagtcg actaatttgc tttccagcgt gtcgagggct
     2701 tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca tttcactgat aaccgccagc
     2761 aaaggaacct cccagaggat gacttcacgc cacgggccgc ttaaacgaat atccagcttg
     2821 ccattatcgt tggacacggt gacttgttcc gggttaaagc ggaactcgcg taaccagtta
     2881 agatagtcgg ccttaaagaa aggcagggca gaaagccact gatattcatc atcctgcagg
     2941 cgcaggtgct gcatcgcctg aacctgttca cgaatagcat cggcataaat acccagcaga
     3001 tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat cgtaataatg atgaaacacg
     3061 gcttgctgca tatgcaactt ataagcatct gtatccagca acgagtgcag aacaggagaa
     3121 gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag gagcgtttag tacaataaac
     3181 atcttcagga actgctggag tataccttgt ttcgcaattt attgaacccc gatcacacca
     3241 tatgccacct ttctggtcga tggcattacg cggtgtatgt tataaaaatg tagcaataaa
     3301 ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg atttacgcaa caggaataga
     3361 ctgaacacca gactctataa aagatgctaa aggttattta tgactcaaca gccacaagcc
     3421 aaataccgtc acgattatcg tgcgccggat taccagatta ctgatattga cttgaccttt
     3481 gacctcgacg cgcaaaagac ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca
     3541 tcagatgctc cgctttgtct caacggcgaa gacctcaaac tggtttctgt tcatattaat
     3601 gatgagccgt ggaccgcctg gaaagaagaa gagggcgcac tggtcatcag taatttgccg
     3661 gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac ggcgctggaa
     3721 gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg tttccgccat
     3781 attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc
     3841 gataaaacca aatatccctt cctgctttcc aacggtaacc gcgttgcgca aggcgaactg
     3901 gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg ctacctgttt
     3961 gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg ttctggtcgc
     4021 gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc gtgggcgatg
     4081 acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga gtatgacctc
     4141 gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga gaataagggt
     4201 ctgaatatct ttaactccaa atatgtgctg gcccgcaccg ataccgccac cgacaaagat
     4261 tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac cggtaaccga
     4321 gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt cttccgcgat
     4381 caggaattca gctctgacct tggttcccgc gcggtaaacc gtatcaataa tgtgcgcacc
     4441 atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat ccgcccggac
     4501 atggtcattg agatgaacaa tttctacacc ctgaccgttt acgagaaggg cgcggaagtg
     4561 attcgtatga tccacactct gcttggcgaa gaaaacttcc agaaagggat gcagctctat
     4621 ttcgagcgcc atgatggcag tgcagcgacc tgcgacgatt tcgttcaggc gatggaagat
     4681 gcgtcgaatg tcgatctctc ccatttccgc cgttggtaca gccagtccgg tacgccggtt
     4741 gtgaccgtca aagacgacta caatccggaa accgagcagt acaccctgac catcagccag
     4801 cgcacgccag ccacgccgga tcaagcagaa aaacagccgc tgcatattcc gtttgccatc
     4861 gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca tccggtgaat
     4921 tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt ctacttccag
     4981 ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata taagtggagc
     5041 gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttttcccg ttgggatgcg
     5101 gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag
     5161 ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct cgatgagaag
     5221 attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga aatggctgaa
     5281 ctgttcgata tcatcgaccc gattgctatt gccgaagtac gcgaagcact cactcgtact
     5341 ctggcgactg aactggcgga tgagctactg gctatttaca acgcgaatta ccagagcgag
     5401 taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg cctgcgcttc
     5461 cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata ccatgaagca
     5521 aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca gctgccttgc
     5581 cgtgacgcgc tgatgcagga gtacgacgac aagtggcatc aggatggtct ggtgatggat
     5641 aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac ggtgcgcggc
     5701 ctgttgcagc atcgctcatt taccatgagc aacccgaacc gcattcgttc gttgattggt
     5761 gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atgggagcgg ttaccagttc
     5821 ctggtggaaa tgcttaccga cctgaacagc cgtaacccgc aggtagcttc acgcctgatt
     5881 gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat gcgcgcggcg
     5941 ctggaacagt tgaaagggct ggaaaatcta tctggcgatc tgtacgagaa gatcaccaaa
     6001 gcactggctt aataaatccc cgaatggcgg caatagcgcc gccattcggg gattttaccc
     6061 ctgttttctc aggcgagttt cagattcacc acgctgcatc acccgttgca acacttccgc
     6121 ttccagctcc gctaatcgca ccgaccctaa ccggcgcgga cggggaatat ccaccgtcaa
     6181 atccagacca atttttccct cttcaattaa caacacccga tcagccattg ctaccgcttc
     6241 gctcacatca tgcgtcacca acagtacggt aaaaccgtgc tcctgccaaa gtgacacaat
     6301 caaatcctgc atctcgagtc gcgttaaggc atccagcgca ccgagcggtt catcaagcaa
     6361 caataaaccc ggtcgatgaa tcaacgctct tgccagcgcc actcgctgtt tctgcccgcc
     6421 agaaagtgcg gcaggccatt ccccggcgcg attctccagt cctaccgcag ccaacgcccg
     6481 acgcgcggca tcgcgccact ggcctttaag gcctaaccca acgttatcaa tcaccgattt
     6541 ccacggtagc agacgcgcat cctgaaacat cattcgcgtg tcttcctgaa tttcagccag
     6601 cggtgtggtg cccgctaaca catcgccagc ggttggcgtt tccagacctg ccagcaggcg
     6661 cagcagggta cttttgccac caccgctgcg gcccaccacc gccacaaact gacctgccgg
     6721 aatatgtaaa tccagttggt tcaggacgat attttccgcg taatgtttgc ttactgcatt
     6781 gagcaacaat ggtgtgccct ggttcagacg agcagtattc ataccgtggc ctccttcaaa
     6841 tgataagccg ggttccagcg caaccagagg cgctctaaca gctgcgcgct gacgtcagca
     6901 agtttgccga gcagggcgta aagaataata gcgactacca ccacgtccgt ttgcaaaaac
     6961 tcccgcgcat tcatcgccag ataaccaatg cctgaattgg cagaaatggt ttcggcaaca
     7021 atcagcgtca gccacatcag gcccaacgca aaacgcacgc cgaccataat tgagggcagg
     7081 gcaccaggca ggatcacatg gataaacagc ggtatgccgg ataatccata gctacgcgcc
     7141 atctccacca acccgcgatc gatattacgg atgccatgcc aggtgttgat ataaatgggg
     7201 aatagcgtac cgagcgccac cagaaagatt tttgcggatt catcaatgcc aaaccacaaa
     7261 atcaccagcg ggatcagcgc cagatgcggc acgttgcgca acatctgaat tgaggtatcc
     7321 agcagccttt ctccccagcg cgacagtccg ctaatcagtc ccaaaatcaa ccccagcgag
     7381 ccgccaattg aaaaaccaat cagcgcccgc caggagctga tcgccagatg ctgccacaat
     7441 tcgccgctgg ctgagagcgt ccagaacgcc atcaccaccc cttccggtga gggcaaaata
     7501 cgcgtcgaca accagccaac cgaggaggcc agttgccaca ccgtcacgat gcccaccggt
     7561 aaaaaccagg gggcaacgcg caataaccac ttcttcactg gcgttgccat tatcttcttc
     7621 cttagctttg cgcgactctg cgagggataa aatcattctc caccgcttcg ccttgcggat
     7681 tcagcggctg cggctgggga atttccggaa tggcgacatc cagatgtggg aacagcaact
     7741 cgccgacccg atacgcttct tccagatgcg gatagcccga aagcacaaag ctgtcgatgc
     7801 caagcgcggc atattcgttg attcgcgcag cgaccgtagg accatcgccc accagcgccg
     7861 tcccggcacc gccgcgcact aagccaacgc ccgcccataa attggggctg atctccagat
     7921 tgtcgcgctt gccgttatgt agcgctgcca ttcgctgttg ccccacggaa tccgtgcggg
     7981 cgaatgcggc ttgtgctttg gcgatagtct catcatcaag atgcgagatt aaccgctcgg
     8041 cggcctgcca cgcttcgtcg ttagtttcac gaacaatcac atgcagacga atgccgaaac
     8101 gaattttgcg tccatgcgcg gcagctttcg cccgcacttg ttcgattttc tctttaacca
     8161 gttccggcgg ttcgccccag gtgaggtaga gatcaacctg ttctgccgcc agctcctggg
     8221 cgacgtctga cgatccgcca aagtaaagcg gaggatacgg ctgttgaatc ggcgggaaga
     8281 gcagtttggc tccccgcaca tgaatatgtt taccgttgaa atcgacggtt tcccccagca
     8341 ataaacgccg ccagacctgg gtaaattccg ccgaggcttc gtagcgctca ctatgatcaa
     8401 ggaacacgcc gtcgcctgcc agttcttgtg gatcgctgcc tgtgaccagg ttaaacaacg
     8461 cacgtccatt tgagagacgg tcaagcgtgg cggcctggcg ggcggcaacg gtaggtgagg
     8521 ttacgctggg acgcagggcg acaagaaact tcagccgctg cgtcaccggg atcatcgatg
     8581 cggcaaccag ccacgcatct tcgcaggagc gccccgttgg gatcagcaca ccggtatagc
     8641 caagacgatc cgccgcttgc gcaatttgtt gcagataacc gtgatcaacc gggcgtgaac
     8701 cttcttccgt tcccagataa tgcccgtcac catgggtcgg taaaaaccag aacatattca
     8761 gactcataat tgttttcctt ccagttgagt gggctgccag atgcgttggc gaatatcgac
     8821 ttttttcggc accagacgat tttcataaaa cagatctgcc gtttgctgct gtaaggcggc
     8881 aacctcggcg ttaaccggtt tgatggtggt aggagggcga tgatctaagt aagaggcaat
     8941 caccggtgcc ggtaagccca tcgtttttgc cagtaaagcg atactttgct cgcgctggct
     9001 gcgggttaac gcatcggcct cactaaaggt tgccagtaca ccctgaataa aagcgccgtt
     9061 tttttctgca taggggcgag ctgccagata aaacgatccg gtttgattga gatcggtgcc
     9121 gtctttcaac acccgcacgc cgccctgtaa taatgcagcg gagtagtagg gatcccagat
     9181 agcccaggcg tcaacgttac cttgctggaa cgcggcgcgg gcatcagctg gcgtcagata
     9241 agtgggctgg atgtcagtaa acttaagtcc ggcctggcgt agtgcgcgca gtaaaagatt
     9301 gtgtgaactg gaacctttct ggaacgcaac tttgtggcct ttaagatcgg ctgcggtttt
     9361 gatcgggctg ttttctgcca ccagaatcac ctcggctttt ggcttcggcg gctcgacgcc
     9421 cacgtacagc aaatcagccc cggcagcctg ggcgaagatt ggcggaatat ccccggtact
     9481 gccgagatca atactgccaa cgtttaacgc ctcaagcatt tgcgggccag cggggaactc
     9541 cacccacgag atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccaa
     9601 taccatacca atactgcctt tctgatagcc tatacgtaac gcttcaggcg aggattctgc
     9661 agcaaccaca atcgtagaga cgctaagcaa tcccaccagc gccagtttaa tgatgttacg
     9721 catgggcatt acctcgcaga gaatgaagat caggaacctg aacatcgcgg cggtgcaatg
     9781 cctgccagaa agtttctagc gcggtatcaa gacgggtttg taaattcggc gtgaactggg
     9841 gtttgtgatg gtaatcaatt acttgtgagt catcggcaaa cacgccgtgc aggatctcct
     9901 gggctttcag cgcacttaaa actggcttaa gggcataatc gaccgccagc agatgggcca
     9961 ccgtaccgcc cgtcgccagc ggtagcacca ctttgccttg caacgcacgt tctggcagca
    10021 ggtcgagcag ggttttcagc gcaccggaat aagcggcttt atacacaggc gtggcgacaa
    10081 tcagcccgtc agcctgttgc aactgttcgg tgaaggtatg gagtgccgga ctatcgaaac
    10141 gagcataaag cagatcttcc gcggcgaagt tttgcagatt ccagtgataa acctcaacat
    10201 ccaggccatt tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaagc
    10261 gaggactacc cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttcttgt
    10321 tttgttaaca ttgataacaa ttcgctcagt ctgtcggaga gacaagaaaa tgccaaatat
    10381 aaattttgtg tatctttttc tgaaaataaa ctaaagaaag ggttttatat atcacgcata
    10441 tttatttatt ggatagtcat tagatgttga atcaatgaat tactcgttca ggtaatattt
    10501 aaatattcat gaaatctata aattaaagat ttgtcactta ttggatttag tatgcttgat
    10561 ttaaatggcg gacaattatt gttattatta agtctaatgt catttaaagg cattatcata
    10621 tttacgaaga gatcaggatg ataacgatga aaaaaagtgt attgacggcg tttataactg
    10681 tggtatgtgc aacgtccagc gttatggctg ctgatgataa tgctatcacg gatggctcag
    10741 taacatttaa tggtaaagtt attgctccag cttgtaccct ggtagctgcg acgaaagatt
    10801 ccgtggtgac tttgccagat gttagtgcca cgaagttgca aaccaatggt caggtttctg
    10861 gcgtgcaaac tgatgtgcca attgaattaa aagattgtga tactaccgta acaaaaaatg
    10921 caacgttcac ctttaatggc actgcggata ctactcagat tacagcgttt gctaaccagg
    10981 cctcatctga tgctgctaca aacgtggccc tgcaaatgta tatgaatgat ggtacaacgg
    11041 ccatcaagcc agacacagaa accgggaaca ttttgttgca agatggagat cagacgttga
    11101 cttttaaagt tgattatatc gctacgggga aagcgacttc tggtaatgtg aatgcggtaa
    11161 caaatttcca tattaactat tattaataga actcattaat tgttttatta attagtaccc
    11221 ctccagtgtt atggagggga tattcatatt ttttaagagt gactatttat gaaaacttgc
    11281 ataacaaagg gaattgtgac cgtaagttta acggcaatat tactcagctg ttcgtccaca
    11341 tgggcggcag gtaaaggcgg ggttggactt gcagcaacac gtcttgttta ttcggaaggt
    11401 gaagagcaaa tttcactggg tgtacgtaac accagtccgg atgttcctta tctgattcag
    11461 tcatgggtga tgaccccaga taataaaaaa tcagcggact ttattattac accaccgcta
    11521 tttgtgctga atccggcaaa cgagaatctg ttacgcatta tgtacattgg cgcgccgttg
    11581 gcgaaagaca gagaaactct tttcttcact agcgtacggg cagtcccttc aacaacgaag
    11641 cgggaagaag gcaataccct gaagattgcc acacaaagcg tcatcaaact tttctggcga
    11701 ccaaaaggtt tagcgtatcc cttaggcgag gctccggcga aactgcgttg cacttcgtca
    11761 gctgacatgg ttacggtcag taacccaaca ccttatttca ttaccctgac agacctgaaa
    11821 ataggtggaa agttagttaa aaatcaaatg atttccccct ttgataaata ccaattctct
    11881 ctgccaaagg gtgccaaaaa tagcagcgta acgtatcgaa ccatcaatga ctacggggcg
    11941 gaaacgccgc aactcaactg taaatcgtaa ttcgtcttca gttaagagag cgagatgtat
    12001 agaactcacc gacaacacag cctgttaagc tctggtggag tgccatcgtt tattggtggg
    12061 ctggtggtgt ttgtgtcggc agcgttcaat gcacaagctg aaacctggtt cgatccagcc
    12121 tttttcaaag atgatccctc aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa
    12181 ataacgccag gggtttatcg tgtcgatatt gttctgaatc agacaattgt agatacgcgc
    12241 aacgtcaatt ttgttgagat aacgccagag aaggggattg ccgcctgttt gacgactgaa
    12301 agcctggatg caatgggcgt gaatactgat gcatttccgg cttttaaaca actggacaaa
    12361 caagcgtgtg cgccattggc ggagattatt ccggatgcca gcgtaacttt taatgtgaat
    12421 aaactccgtc tggaaatttc agtaccgcaa attgccatca aaagtaatgc tcgtggttat
    12481 gtccccctga acgttgggat gaagggatca acgcgctatt actgggatat tcatttagcg
    12541 gggctaacag tattcatagc agcgcagaca gtgattctgg cgacagttat tttctgaatt
    12601 taaacagtgg cgttaattta ggcccatgga gattgcgcaa caattcaaca tggagtcgta
    12661 gtagtggcca aaccgcagaa tggaagaatc tcagcagcta tttgcaacgg gcggttattc
    12721 ctctgaaagg cgaactgacc gtaggtgatg attatactgc aggcgatttt ttcgatagtg
    12781 tcagctttcg tggtgtgcag ctggcgtcag atgacaacat gctgccagac agcctgaaag
    12841 ggtttgcgcc tgtggtgcgt ggtatcgcca aaagcaatgc ccagataacg attaagcaaa
    12901 atggttacac catttaccaa acttatgtat cgcctggtgc ttttgaaatt agtgatatct
    12961 attccacgtc gtcgagcggt gatttgttag ttgaaatcaa agaagcggac ggcagcgtca
    13021 atagctacag cgtacccttt tccagcgtgc cattactcca gcgtcagggg cgaatcaaat
    13081 acgcggtgac actggcgaaa tacagaacca atagtaatga acagcaggag agcaaatttg
    13141 cccaggccac gttgcagtgg ggcggaccgt ggggaacgac ctggtatggt ggtggacaat
    13201 atgctgaata ttaccgtgcc gccatgtttg gtctgggatt taacctcggc gatttcggag
    13261 caatttcgtt cgatgtgacc caggcgaaga gtacgctggc agaccaaagc gaacataaag
    13321 gtcagtcata tcgttttctg tatgccaaaa cgctcaacca attgggtact aactttcaat
    13381 tgatgggcta tcgctattcg acgtcgggtt tctacaccct ttccgacacc atgtataaac
    13441 atatggatgg ctacgaattt aatgacggtg atgatgaaga tacgccgatg tggtcgcgtt
    13501 attacaattt gttttacacc aaacgtggca aactgcaggt caatatctcc cagcaattaa
    13561 gcgagtacgg ttcgttttat ttaagtggta gccagcaaac ttactggcat accgatcaac
    13621 aggatcggct attacagttt ggctataaca cgcaaattaa agatctctcg ctggggatct
    13681 cctggaacta cagtaagtcc cgtggccaac ctgatgctga tcaggtgttt gcacttaatt
    13741 tttccctgcc actcaatctg ttgctctcca gaagtaatga tagctatacc agcaaaaaaa
    13801 attacgcctg gatgacctct aacaccagta tcgataacga agggcacact acacaaaacc
    13861 tgggtttaac ggagacacta ctcgatgacg gtaacctgag ctacagcgtg caacagggat
    13921 ataacagcga ggggaaaacg gctaatggca gcgccagcat ggactacaaa ggggtgtttg
    13981 cagatgcccg agtgggctac aactacagcg ataacggcag tcaacaacaa ctgaactacg
    14041 ctctttcagg cagtttagtt gcccattcac agggcattac cctggggcaa tcactggggg
    14101 aaactaacgt tctgattgca gcaccaggcg cagagaatac tcgtgtggcg aacagcaccg
    14161 ggctgaaaac tgactggcgc ggatataccg ttgttcctta tgccacttct tatcgtgaaa
    14221 atcgaatcgc acttgatgcg gcgtcgttaa aacgtaacgt ggatcttgaa aatgcagtag
    14281 tcaacgtggt tcccaccaaa ggggcgttgg ttctggcgga gttcaatgcc catgcgggag
    14341 caagggtatt aatgaaaaca tcaaagcagg gtatatcgct gcgttttggc gcgatagcga
    14401 cgctggacgg cgtacagact aatagcggca taattgatga tgatggctcg ctctatatgg
    14461 cgggtttacc ggcgaaggga acaataaccg tgcgctgggg cgaagctccc gatcaaattt
    14521 gtcatatcag ttacgagctt accgaacaac aaattaactc tgcgattacg cgaatggatg
    14581 ccatatgcag ataatctttg gagaaaaatg cgtgtcatta ctacgactat tttttgccgc
    14641 cgtcttaatg ctatggtgcg ctcaaaccgc tgcttatagc gggcagtgtc ataccactca
    14701 ggggaatccg tatattggcg tcaattttgg cgttaaaacc ctggaggaag aagaaaatac
    14761 gactggggta gtaaaagaca aattttatca gtggaacgaa tcgaatgatt attatgtttc
    14821 ctgtgattgc gataaagaca atgtcagaag tggccgatgg gcattcgccg cggattcacc
    14881 gttagtctat ttaggcgaca actggtacaa aattaatgac tatcttgccg ccaaagtttt
    14941 attgcaggtt aaaggcagtt ctcctacagc ggttcctttc gaaaacgtgg ggactggggc
    15001 agatacccgg tggcatattt gtgaccccgg cggtcaacgt ttaggcggcc agggagctag
    15061 cggtaatagc ggtagctttt ccctgaaaat attgcagccg ttcgttggtt cggtcgtcat
    15121 tcctcctatg gcgctggcgc gattatttga atgctacaac atacccgcag gtgattcctg
    15181 cacgactaca ggcacaccgg ttttagtgta ttacctgtct ggtactatca attcacttgg
    15241 ctcatgttcc gtcaatgccg gagaaacaat cgaggtcgat ctgggcgacg tatttgcggc
    15301 taactttcgt gttgtagggc ataagcctct tggggccaga acggcagaac ttgcaattcc
    15361 agtcaggtgt aacacgggaa acgcggggtt agttaacgtc aacctgagtc tgacggcaac
    15421 cacagacccc agctatcccc aggcgattaa gacgtcacgt cctggcgtgg gcgtggtggt
    15481 gaccgatagc cagaacaaca ttatttcccc tgctggtgga acattaccgc tctctattcc
    15541 tgatgatgca gacagtatcg cgtgaatgaa tgtctatcca gtcagcacga caggtgtacc
    15601 accagaaacc gggcgatttg aagccacggc aacggtgaga ataaattttg attaacgcgt
    15661 gaacgtatga agaaaaaaaa cgatatttca gtgcgttatc ttgttcttta gcattcttaa
    15721 catccatgtc gggatggctg ggcctgaaca agttagtatg catatttatg ggaatgtggt
    15781 cgatcagggc tgtgatgtcg ccaccaaaag tgcattacaa aatattcata ttggtgattt
    15841 taatatcagt gattttcagg ccgcgaatac cgtaagcact gctgctgatt tgaatatcga
    15901 tatcaccggt tgtgccgctg gtattactgg cgcggacgtc ctttttagcg gcgaggctga
    15961 cacccttgcg ccgacactgc tcaaactaac tgacacaggc ggaagcggtg gtatggcaac
    16021 ggggattgcc gtgcaaattc ttgatgcgca aagtcagcaa gaaatcccgc tcaatcaggt
    16081 ccagcctctt acgcccttaa aagccgggga taacacactc aaatatcaac ttcgttataa
    16141 gtccacaaag gcgggagcaa cgggcggtaa tgcgacggcg gttctctatt ttgatctggt
    16201 ttaccagtga aggagtgatg ttgaaacgga taatctggat tctgttctta ctgggattaa
    16261 cgggggggtg tgagctattt gcccatgatg gcacggtcaa cattagcgga tcgtttcgcc
    16321 gtaatacatg cgtgctggca caggatagca agcaaatcaa tgtgcagtta ggcgatgtct
    16381 cactgacacg tttttctcat gggaattatg gcccggagaa atctttcatc attaatttgc
    16441 aggattgcgg aacagatgtc agcacggttg acgtcacctt ttcaggaacc cccgacggag
    16501 tgcagagcga aatgttgtcc attgaaagtg gtacggatgc agccagcggg ctggcaattg
    16561 cgattctgga tgatgcgaag atattgattc cgcttaacca ggcgagtaaa gactacagcc
    16621 tacatagcgg taaggtgcca ttaacttttt atgcgcaatt gcgacctgtc aatagcgatg
    16681 tgcagtcggg taaagtgaat gccagcgcga catttgtact tcattatgac taatacgtgg
    16741 aacagattgg cgctcttgat tttcgccgtt ttatcgttgc tggtggcggg tgaattgcag
    16801 gcaggagtgg tggtcggcgg aacgcgattt atctttccgg cagacagaga atcgatatct
    16861 attttactga ctaataccag tcaggaatcc tggcttatta acagtaaaat caaccgccca
    16921 acgcgttggg ccgggggtga agcgtcgaca gtgccagcac cattactggc cgctccgcca
    16981 cttattctcc tgaagcccgg tacgacaggc acgttgcgct tgctgagaac ggaaagcgac
    17041 atcttgcctg tggatcgcga aacgctattt gagttaagca ttgccagcgt gccatccggc
    17101 aaagttgaaa atcagagcgt aaaagtggcg atgcgctcgg tatttaaact gttctggcga
    17161 cccgaaggtt gccgggcgac ccgctggaag cttatcaaca attacgctgg acacggaagt
    17221 cgcagggtgt acaactcact aacccaacgc cttattacat taacctgatt caggtgagcg
    17281 taaatggtaa agcgttaagt aatgtgggag ttgtgccgcc taaaagccag cgtcagacaa
    17341 gctggtgtca ggcaatagca ccgtgtcatg ttgcctggcg ggcgataaat gattacggcg
    17401 ggttgagtgc aaagaaggag caaaatctgc cctgaaacag gttcggaaaa cgtttgcgtt
    17461 ttttttgccg caggtcaatt cccttttggt ccgaactcgc acataatacg cccccggttt
    17521 gcacaccggg aatccaggag agttcatgta ctaccccttc gttcgtaaag cccttttcca
    17581 gctcgatcca gagcgcgctc atgagtttac ttttcagcaa ttacgccgca ttaccggaac
    17641 accgtttgaa gcactggtgc ggcagaaagt gcctgcaaaa cctgttaact gcatgggcct
    17701 gacgtttaaa aatccgcttg gtctggcagc cggtcttgac aaagacgggg agtgcattga
    17761 tgcgttaggc gcgatgggat ttggatcgat agagatcggt accgtcacgc cacgtccaca
    17821 gccaggtaat gacaagccgc gtctctttcg tctggtagat gccgaaggtt tgatcaaccg
    17881 tatgggcttt aataatcttg gcgttgataa cctcgtagag aacgtaaaaa aagcccatta
    17941 tgacggcgtc ctgggtatta acatcggcaa aaataaagat acgccagtag agcagggcaa
    18001 agatgactat ctgatttgta tggaaaaaat ctatgcctat gcgggatata tcgccatcaa
    18061 tatttcatcg ccaaataccc caggattacg cacactgcaa tatggtgaag cgctggatga
    18121 tctcttaact gcgattaaaa ataaacaaaa tgatttgcaa gcgatgcacc ataaatatgt
    18181 gccgatcgca gtgaagatcg cgccggatct ttctgaagaa gaattgatcc aggttgccga
    18241 tagtttagtt cgccataata ttgatggcgt tattgcaacc aataccacac tcgatcgttc
    18301 tctggttcag ggaatgaaaa attgcgatca aaccggtggc ttaagtggtc gtccgcttca
    18361 gttaaaaagc accgaaatta ttcgccgctt gtcactggaa ttaaacggtc gcttaccgat
    18421 catcggtgtt ggcggcatcg actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc
    18481 ctcactggtg caaatttatt ctggttttat ttttaaaggt ccgccgctga ttaaagaaat
    18541 cgttacccat atctaattat ttcttcgaat tcctctttat aagataatca gggctttatt
    18601 ttcagccctg gttgttttat attcattgct gttgcttatt tagacatttt gtacttttat
    18661 tgttgaggtt attaagcgaa gcgacaatgg attgtgttgc tgcggtttta tagggtaggg
    18721 gagaggcaga tgcgaattaa accagacgat aactggcgtt ggtattacga tgaagagcac
    18781 gatcgtatga tgctcgattt agccaatggt atgctatttc gctcacgttt tgcgcgcaaa
    18841 atgttgactc cagatgcctt ttctcccgca ggcttttgcg ttgacgacgc cgcgctctat
    18901 ttctcttttg aagaaaagtg ccgcgatttt aatttatcta aagaacaaaa agccgaactg
    18961 gtgctgaatg cactggtagc aattcgctat ctcaaaccgc aaatgccgaa aagctggcat
    19021 tttgtttccc atggtgaaat gtgggtgccc atgccgggcg atgcggcctg cgtttggttg
    19081 agcgacacta acgagcaagt gaatttactg gtcgttgaat ctggcgagaa tgccgcgcta
    19141 tgcctgctgg cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc
    19201 attaaaatca tgaacgacag gctgaaaccg caggttaatg ttgacagctt cagcctcgaa
    19261 caggcagtct aacgcgccaa cttaagtgca gtcttcggta cacagctaca gcaaagaatg
    19321 gtgccatcat cgcccattgc tgattttttc agcggcgtga cttcgccttc taaaagctga
    19381 acacggcaac ttccacaaat gcccgcgcgg caagaataag ggatacgaat tccctgattt
    19441 tctaattgct ccagcaacac ctgttggtta tttccacgaa atgcctgtcc ctgccagtca
    19501 atatctacat tggcgtccgg ttgttgcgtg atgttggcag tatcatcagc ggcagctgcg
    19561 ccgtaaattt ttgccggagc cgttgtcaga atttccacct catcgccaac gcgaatcacg
    19621 ccgctattac gggcaattaa attctgacca aaatcgacat cgccgttatc ctgggcagtg
    19681 cggaaagatt gcaatgtttt taatggttcg cctgccggat gtttttgccc tttttctggg
    19741 ctgacggtgg tgaaaataca gcggctacaa ggtttaacca catcaaacac cacatcacca
    19801 atgcgaatca ctttccaact atcttcttcc caggctgacg cgccggaaac caccagattg
    19861 gggcggaatt gctccatttt tacactggcc ggacaacgtt gttggagatc acgtaacgag
    19921 gcttcgttag caagaaggta aggatagcca tcagcaaatg acaggggtac agtgttgtgg
    19981 cgtttcacgc gccgggtcat ttgtggcccc acccagcgta attgcacttc gcgggagaaa
    20041 aatccactta accatttgtt gatcgcttct ggcgcaattc gcgcggtaaa atgtgtgccc
    20101 caaacttcgg ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg
    20161 ctatctggtg cggtgagatg caagccatca tgcacgggtg aaggagtaaa tcgtaccatc
    20221 tggggaaact ggcgagcggt gataaacgta ccgtcaggtt ccgtgatcat aaagatgcga
    20281 tcgaaggcca gaccactgac atctgccaga gcatgtgtaa gaccaatgcc gcgcatcgat
    20341 ttaacaggat gaataaaaag ccgggttaat gtcgccacgg tgcggtcctc aaatgaaaat
    20401 aagccctcaa ctttatgaca tgacgcgctt attagctata atgcgcaaca attttcttag
    20461 cgcctgtccc attaggctat tttatttgtc agtttggccc tgggttgtgc tcgaaatcct
    20521 cacgtacttg tgtacgctcc ggtttctccg cgctgtccat gtccagactg actgcaacaa
    20581 ttacgcctac tgcggtaggc tcttagagta aaagtgacga tatgaattct ctgtttgcca
    20641 gtacggcccg tgggctggaa gagctgttaa aaactgaact ggaaaacctg ggggccgttg
    20701 aatgccaggt ggttcagggt ggggtccatt tcaagggcga cacacggctt gtttaccaga
    20761 gcctgatgtg gagccgcctg gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt
    20821 acagcgattt agacctctat ctcggtgttc aggcgatcaa ctggacagag atgtttaatc
    20881 ctggcgcgac cttcgctgtc cacttcagtg gtttgaatga cactatccgc aacagtcagt
    20941 acggcgcgat gaaagtgaaa gacgcgatcg tcgatgcttt cacgcggaaa aatctgccgc
    21001 gtccaaatgt tgatcgcgat gcgccggata tccgcgttaa cgtctggctg cataaagaaa
    21061 ccgccagtat cgcccttgat ctcagtggtg atggtttaca tctgcgtggc tatcgcgatc
    21121 gtgctggtat tgcgccgatc aaagaaaccc tggcagccgc tattgtgatg cgatccggct
    21181 ggcagccagg aacaccgttg ctcgatccga tgtgtggttc cggtacgttg ctgattgaag
    21241 cggcgatgct ggcgaccgat cgcgcaccag gcttgcaccg tgggcgttgg ggctttagcg
    21301 gctgggcgca gcatgatgaa gctatctggc aggaagtgaa agcggaagcg caaactcgcg
    21361 cccgtaaagg cctggctgag tatagctctc atttttacgg ttcggacagc gacgctcggg
    21421 tgattcaacg tgcacgcact aacgcccgtc ttgcggggat tggtgaactg atcacctttg
    21481 aggtgaaaga tgtcgcgcaa ctgaccaatc cgctgccgaa agagccgtac ggcacagtgt
    21541 taagcaaccc gccatacggg gaacgtctgg acagcgaacc ggcgctgatt gcgctgcata
    21601 gcctgctggg tcggatcatg aaaaaccagt ttggtggctg gaatctctct ttgtttagtg
    21661 cctcgccgga tctgctaagc tgcttgcagc tgcgtgcaga caaacagtac aaggcgaaaa
    21721 acggcccgct ggactgcgta cagaaaaatt accacgttgc cgaaagcact ccagacagca
    21781 aaccggcgat ggtagcggaa gactacgcca accgcctgcg taagaacctc aaaaaattcg
    21841 agaagtgggc tcgtcaggaa gggattgaat gttaccgcct gtatgacgcc gatctgccgg
    21901 aatataacgt tgccgttgac cgttatgccg actgggtggt ggtgcaggag tatgcgccgc
    21961 caaaaactat tgatgcgcac aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca
    22021 tttcggtact ggggattgcg ccaaacaaac tggtgctgaa aacccgtgaa cgtcagaagg
    22081 gcaaaaatca gtaccagaaa ctgggcgaga agggcgagtt tctggaagtt accgaatata
    22141 acgctcactt gtgggtgaac ctgacggatt atctcgatac tggtctgttc ctcgatcacc
    22201 gtatcgcccg tcgtatgctc ggccagatga gcaaaggcaa agatttcctc aacctgttct
    22261 cttataccgg cagcgccacc gtgcatgcgg gattaggcgg tgcacgcagc accaccaccg
    22321 tggatatgtc gcgtacttat ctggagtggg cagaacgcaa cctgcgtctg aatggcctaa
    22381 ccgggcgtgc gcatcgcctg attcaggccg attgcctggc gtggctgcgt gaggcaaatg
    22441 aacagttcga tctgatcttt atcgatccgc caaccttctc taactcaaaa cgaatggaag
    22501 atgcgtttga tgttcagcgc gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc
    22561 gtgcaggtgg gacgatcatg ttctcgaaca acaaacgtgg cttccgtatg gatctcgacg
    22621 gcctggcaaa actgggactg aaagcacaag aaattacgca aaaaacgctc tcccaggatt
    22681 tcgcccgtaa ccgccagatc cacaactgct ggctgattac cgcagcctga aaggaatagt
    22741 aatgtcatta atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga
    22801 taacgcagaa ctgcatatcg aagataacga acgtgtttgt ctggtgggcc gcaacggcgc
    22861 aggcaaatcg acgttaatga aaatcctcaa ccgtgaacaa gggctggatg acggtcgcat
    22921 tatttacgag caggatttga ttgtagcgcg tctgcaacag gatccgccgc gtaacgttga
    22981 gggtagtgtt tatgatttcg ttgccgaagg cattgaagaa caagcggaat acctgaaacg
    23041 ctatcacgat atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact
    23101 ggcgaaggtt caggaacagc tggatcacca caacctgtgg cagctggaaa accgcatcaa
    23161 cgaagtgctg gcgcaactgg ggttagatcc taacgttgcg ctgtcgtcgc tttccggcgg
    23221 ctggttgcgt aaagcggcat taggacgcgc gctggtgagc aatccgcgcg tattgctgct
    23281 ggacgaaccg actaaccacc tggatatcga aaccatcgac tggctggaag ggttcctcaa
    23341 aaccttcaat gggacgatta ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc
    23401 gacgcgcatt gttgatctcg atcgcggcaa gctggtgacc tatccgggca attacgatca
    23461 gtacctgctg gaaaaagaag aagccctgcg cgtggaagaa ctacaaaatg ccgagtttga
    23521 tcgcaaactg gcgcaggaag aagtgtggat tcgccagggg atcaaagcac gccgtacccg
    23581 taatgaaggc cgcgtacgcg ccctgaaagc gatgcgtcgc gagcgtggcg aacgtcgcga
    23641 agtgatgggt accgcaaaga tgcaggtgga agaggccagc cgctccggta agatcgtttt
    23701 cgaaatggaa gacgtttgct accaggttga cggaaagcaa ctggtgaaag atttttctgc
    23761 ccaggttcta cgtggcgaca aaattgccct gattgggccg aatggctgcg gcaaaaccac
    23821 gctgctgaaa ctgatgctcg gtcagcttca agcggacagc gggcgtattc acgttggcac
    23881 caaactggaa gtggcttatt tcgatcagca ccgcgcggaa ctggatcccg ataaaacggt
    23941 gatggataac cttgccgaag gtaagcaaga ggtgatggtt aacggcaagc cacgccacgt
    24001 tttgggctat ttgcaggact tcctgttcca tccgaaacgg gcgatgacgc cagtacgtgc
    24061 gctttctggc ggtgagcgga accgcttgct gctggcgcgt ttgttcctca aaccaagcaa
    24121 cttattgatt cttgacgaac cgaccaacga tcttgatgtc gaaacgctgg aactgctgga
    24181 agaactgatc gacagctacc agggcacggt attgctggta agccacgatc gtcagtttgt
    24241 cgataacacc gttacagaat gctggatctt cgaaggcggc ggtaaaattg gtcgttatgt
    24301 cggcggttat catgatgccc gtggtcagca agagcagtat gtggcgctca aacagcctgc
    24361 ggtgaaaaaa aacgaagaac ccgccgcgcc aaaagcagaa actgtaaaac gcagcagtag
    24421 caaactaagc tataaattgc agcgcgaact ggagcagcta ccgcaattgc tcgaagatct
    24481 ggaggcgaag ctggaagccc tacagacgca ggtggcggat gcttccttct tcagtcagcc
    24541 gcatgagcag acacaaaaag tgctcgccga tatggctgct gcagagcagg agctggagca
    24601 agcctttgaa cgctgggagt atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag
    24661 tcgatttaaa cagagacagc tgacaacggt aaaattgcct gatgaactgg gtttatcagg
    24721 catgaaaaca aacactttgt tagtcatctg taaaatagcg catcattaag gagtaccaat
    24781 gtgcgaacat catcatgccg cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc
    24841 gttaccgcgc cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca caacgttaac
    24901 cgtggcgtgg gatgcccccc ggcagcgtcc gaccgcctat gcgttggctg cactgttcat
    24961 gctgttgctg tccaacttgt ttccttttgt gaatatgaac gttgcaggag ttaccagtga
    25021 aattacatta ctggaaattc ccggcgtgct tttttctgag gactacgcca gcctcggcac
    25081 ctttttcctg ttgtttgtgc aactggttcc cgcgttttgt ctgataacca ttctgttact
    25141 ggtgaatcgc gcggaattac cggtccgttt aaaagagcaa ctggcacggg tgctttttca
    25201 actcaaaacc tggggaatgg cggagatttt cctcgcgggt gtgctggtca gtttcgttaa
    25261 actgatggct tacggcagca ttggcgtagg cagcagcttt ctcccctggt gtttattttg
    25321 tgtcctgcaa ctgcgcgctt ttcagtgcgt tgatcgtcgc tggttatggg acgatatcgc
    25381 cccgatgcca gaactgcgcc agccgctaaa accaggcgtc acggggatac gtcaggggct
    25441 gcgttcgtgc tcctgttgta cggcaatcct tcctgctgat gaacccgtgt gcccgcgctg
    25501 tggtactaaa gggtacgttc gacgtagaaa cagcctgcaa tggacactcg cgctgcttgt
    25561 tacgtccatc atgctgtatc ttccggctaa tattttgccc atcatggtga cggatttatt
    25621 aggctcgaag atgccatcga cgattctcgc tggggtcatc ctgttatgga gcgaaggctc
    25681 ttatcccgtc gctgcggtga tctttctggc cagtattatg gtgccaacgt taaagatgat
    25741 cgccatcgcg tggctgtgtt gggatgccaa agggcatggc aagcgcgaca gtgaaagaat
    25801 gcatttgatt tatgaagttg ttgagtttgt aggccgctgg tcgatgattg acgttttcgt
    25861 tatcgcggtg ctctcggcgc tggtgcgtat gggaggttta atgagtattt atccggcaat
    25921 gggtgcatta atgtttgctt tagtcgtcat aatgacaatg ttttctgcta tgacgtttga
    25981 cccgcgtttg tcgtgggatc gtcaacctga atcagagcat gaggagtcct gacagtatgg
    26041 aatctaataa tggggaagcc aaaatccaga aagtgaagaa ctggtctccc gtgtggatat
    26101 ttcctatcgt cacggcgctc attggggcct gggttctttt ttatcattac agccatcagg
    26161 gaccggaagt gaccctgatc accgcgaatg cggaaggaat tgaaggtggc aaaaccacca
    26221 ttaaaagccg tagcgttgac gtcggcgtgg ttgaaagcgc cacactggct gatgatttga
    26281 cacacgttga aatcaaagcg cggctgaatt ccggtatgga aaaattgctg cataaagata
    26341 ccgtcttttg ggtggtgaaa ccgcagattg gtcgagaagg gattagcggt ctgggaaccc
    26401 tgctgtctgg ggtttatatc gaactgcagc caggcgcgaa aggcagcaaa atggataaat
    26461 acgatttgct ggactcgcca ccgttggccc cgcctgatgc gaaaggtatc cgtgtggttc
    26521 tcgatagcaa aaaagccggg cagctctcgc caggagatcc ggtgctgttc cgtggctatc
    26581 gggtaggttc ggttgaaacc agcaccttcg atacgcaaaa acgcaatatc agttatcaac
    26641 tgttcatcaa tgcaccttat gaccgactgg tgaccagcaa tgttcgcttc tggaaagata
    26701 gtggcattgc ggttgatctg acgtcagcgg gaatgcgtgt ggagatgggc tcattgacaa
    26761 cgctgctgag tggcggtgtc agctttgatg tgccggaagg tctggattta gggcagccag
    26821 tggcaccgaa aacagctttc gttttgtatg atgatcagaa gagcattcag gattcgttgt
    26881 acaccgatca cattgattat ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg
    26941 gagctccggt agaattccgg ggtattcgcc tcggtaccgt aagcaaagtg ccattctttg
    27001 cgccgaatat gcgtcagaca tttaacgatg attaccgtat tccggtgctg attcgtatcg
    27061 agccagagcg gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag caccttggcg
    27121 aattgttgaa acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc actggcgcac
    27181 tgtatgttga tctcgatttc tatccaaata cgcctgcaat aaccggtatt cgtgaattta
    27241 atggttatca gattatcccg accgttagcg gcggcctggc gcaaatccag caacgactga
    27301 tggaagcgtt ggataagatc aacaaactgc cattgaatcc gatgattgaa caggcaacca
    27361 gtacgctttc tgaaagtcag cgcacaatga aaaacctgca aacgacgctg gatagcatga
    27421 acaagatcct cgccagccag tcgatgcagc agttgccgac ggatatgcag tcaacgttgc
    27481 gtgaattgaa tcgcagcatg cagggcttcc agcccggctc cgcagcctac aacaagatgg
    27541 tggcggatat gcagcgcctt gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc
    27601 tcaatgagaa gagtaacgcg ctggtatttg aagcgaagga caaaaaagat ccagagccga
    27661 agagggcgaa acaatgaaaa agtggctagt gacgattgca gcactgtggc tggccggatg
    27721 cagctccggc gaaattaata aaaactatta ccagttacct gtggtgcaga gcggtacaca
    27781 aagtaccgcc agccagggca atcgcctgtt atgggtagag caggtcgctg tacctgacta
    27841 tctggcgggg aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa
    27901 caacttgtgg gccagcccgt tggatcaaca gttgcgcaac accctggtag ccaacctgag
    27961 cacgcaactg cccggctggg tggttgcctc ccagcctctg ggaagcgccc aggacacgct
    28021 caatgttacc gtaacggagt ttaacggtcg ctatgatggc aaggtcattg tcagtggtga
    28081 gtggctgttg aaccaccagg gacaactgat caaacgtccg ttccgtctgg aaggagtgca
    28141 aactcaggat ggttacgatg agatggttaa agtgctggcc ggtgtctgga gtcaggaagc
    28201 cgcttctatt gcacaagaga taaagcgtct accttaatta taaagatttg taaatataac
    28261 cgtctccggt atgttgcctg aggcggtttt tttgtttcta acgtgcggaa aaatttgttc
    28321 ctcttcacat tttttgtaca accgacatgc ccgtgtagct cacaaatatg acagtggcgt
    28381 gaattttgcg cattgacggc agttatgatt cgcggtattg cttaactgtg attgcacatt
    28441 tagtaatcac tgttttcttt tccaccagaa accagtatga gggaaacgag gcatgaagag
    28501 acaaaaacga gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg
    28561 acgctcaaaa gaaatgtgtc cctatcagac gctgaatcaa aggtcacaat ggctgggagg
    28621 ctggcgagaa gccatggcgg acagggtagt aatggcctga ttctgtctct ttaaaaagaa
    28681 acctccgcat tgcggaggtt tcgccttttg atactctgtc tgattataat cagaaggcag
    28741 acgtatcctg gaacagaccg actttcaggt cgctggcggt atagatcaga cgaccatcaa
    28801 ccagcacttc gccatccgcc aggcccataa tcagacgacg gttaacaatg cgtttaaagt
    28861 gaatacggta ggtcactttt ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc
    28921 caacgcccag cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag aaccctacca
    28981 gctgccacat tgcgtccagg cccaggcatc ccggcataac cggatcgcca ataaagtggc
    29041 atccgaagaa ccacagatcc ggattgatat ccagttctgc ttcaacatac cctttgtcga
    29101 agttaccacc cgtttcggtc attttgacca cacggtccat catcagcatg ttcggtgctg
    29161 gcaattgcgg gcctttagcg ccaaacagtt caccgcgacc agaggcaaga aggtcttctt
    29221 ttgtatagga ttcgcgttta tctaccatgt tctctgtaag ccttatttta ttgaagcacg
    29281 caggatagct aacacgtgta cgctgaacaa gtccgatcag ttcggaataa accagttcag
    29341 ccaacgtaat ggccatggaa aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg
    29401 ttcctggatg gtttgcatca gcgtcgtttg gccttcgcca tcccacacca gatttaataa
    29461 taacggcagt gcgtcagtca catcgtctac tgcccagatg gtgaatttgc cttcttctac
    29521 cgctttcacc agttcactgt gaagacttaa atggcgaacg ttagctgtgg ggataatgac
    29581 accttgtttc ccggttaact cacgttgctg gcaaatagca aagaagcctt cgattttctc
    29641 atttaaacca ccgaccggct gggcgcgacc gaactgatcg actgaacctg tgatagcgat
    29701 actctgattc accggcacat cggcgagggc gcttatcagg gcgcagagtt cagccatcga
    29761 ggcactatct ccatcaactt cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg
    29821 gatctgttgc tcaagctgta gttccgacat caggaacgct tgcatgatca tcatcccttt
    29881 cgcatggata ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc
    29941 aatatgcaca acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa
    30001 ttcaatgacc gaaagggcgt taatttgccc gatgcgttcg ccttcggttt caatcaggat
    30061 ttgctcctga aggatctcat cctgcatacg ttcagcgagg aaaccttcgc gccattcacg
    30121 ctgctgcagc attaagttta gctgctcgcc ggagaaggtg tcgccatcac acagggaggc
    30181 gacctcttta cactggcgga ggatccactg cgggctaagc ggaagtgttt cttgttcacc
    30241 ggtgtagcgt gctgcttcgc ggataagtac cggccaggca tccgctcccg gggcaggtaa
    30301 gtgattatgt ctggcggtaa atgtcaccca gcgacaccac tgggttactg actccgcatc
    30361 gacaatctgc agagtatctt caaattcgct ataaatagcc tgctctgaaa gctctggctc
    30421 catctcctgg aaatcagcca atgattcgcg ttcgcctacc agaatgactt tcagcttcaa
    30481 tggcatcgaa ggcacagaga cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa
    30541 acgctcgcgg ttaacgatat ttttcagccg catccacagc agaggttgcg ccagcagtgt
    30601 acgcaaagag atgatgagaa taccgccatt tgcctgatgc accagaccag gctgcagggt
    30661 aatgtcgcca ttaaactggc gcaggcagcc aaagagttgc tccgcttcta cccagtcggc
    30721 agcgacaact tgcgttaaag tcgcaaaatt atcatctgca ctcactgcgt gacgtaagcg
    30781 gatggagtgg ccggaaacct catagtgacc gcccaccagt tggcctgcat cgctttgtag
    30841 cgtacgcgcg gcattggcaa taagattcag atactcagac tcttccgggg ccttcgccag
    30901 cataaaagag gaggatgctc gcgtatgcag caactgctcc agcgcaaatt gcagccgcgg
    30961 ttgagtatca ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag caaatatttc
    31021 ctgatagcta tcggtatcag gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt
    31081 tgatgttttt tagtctgttg tcaaagccgc gattataccg taaccggcac tacagcacac
    31141 gtagaaagca ccgacaatac tcctggcatg ggcgttaaag ctcacaggat ggagattctt
    31201 ttcttcactg gcctaaaaag ctgatattct gtaaagagtt acacggtaac attgagatcg
    31261 ctatgaaata tcaacaactt gaaaatcttg aaagcggttg gaaatggaag tatctggtga
    31321 agaagcatcg cgaaggggag ttaatcaccc gttacataga agccagtgcc gcccaggaag
    31381 ccgtcgatgt gttgctctcg ctggaaaatg aacccgtgct ggtaaatggc tggattgaca
    31441 agcatatgaa tccggaactg gtcaatcgca tgaagcaaac cattcgggca agacgaaagc
    31501 gccattttaa tgcggaacac cagcatacgc gcaaaaaatc gatcgatctg gaatttattg
    31561 tctggcaacg tctggctggt cttgcgcagc gtcgcggtaa aacgctttct gaaacgattg
    31621 ttcagctgat tgaagatgcg gaaaacaaag agaaatacgc gaataaaatg tcttctctga
    31681 agcaggatct gcaggcattg ctgggtaagg aataactgac gaaagtcagt tcaatttact
    31741 aaaggcaaaa aaaccccgca gcagcggggt ttttctacca gacgagaact taagcttgcg
    31801 gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca
    31861 ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
    31921 tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt
    31981 aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga
    32041 tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct
    32101 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt
    32161 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
    32221 gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa acacccaggc
    32281 tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc
    32341 actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccgccagcga
    32401 agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta gtgtctgcac
    32461 gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca
    32521 gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt
    32581 acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt
    32641 aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg ttgttgatga
    32701 aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt
    32761 tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg
    32821 tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag
    32881 ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc
    32941 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    33001 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atcttttttg
    33061 tcagcgacaa tttacagaag agaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    33121 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    33181 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg
    33241 agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    33301 aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacact
    33361 gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag gggagagctg
    33421 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag
    33481 tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa
    33541 tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat
    33601 aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa
    33661 cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta
    33721 ctgtatggat gtacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc
    33781 ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta atcttgccgc ttaagaataa
    33841 gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt
    33901 ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc
    33961 tggcaacgtt gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg
    34021 acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa
    34081 gtgcacagta ctgtgtaaaa tatccgcctg tctggctgac atataaaaag tgtggccgat
    34141 ccgttaccct caattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg
    34201 tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc
    34261 gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagg
    34321 tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt tggttgcgac
    34381 tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa ggtgccatta
    34441 tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca gaatgggctg
    34501 actctcttac gccgaaacag gagtttcctg cggaacttga gtaatctggc gttgcagacg
    34561 accaatctca ggcagtaatg caatcaatag tccaacttgt tgtacgacca gaggttcttt
    34621 gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta atgcctcatt
    34681 gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc aaactgcgtc
    34741 atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga
    34801 gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt cgcgaatttg
    34861 cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct cagcatcacg
    34921 gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta
    34981 ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt cgagcatgcg
    35041 cggcagattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc acgcaatggc
    35101 acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt cacccagtaa
    35161 gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt
    35221 acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc cttccagtga
    35281 tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac caataatcct
    35341 taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag
    35401 caagatccaa tacccgtgat gcattccggt tatctgaatg atggcgtagc cgaagcacaa
    35461 caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct
    35521 aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct cattttcgtc
    35581 attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt gggcatcaat
    35641 ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt
    35701 atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc
    35761 atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg cctggccctg
    35821 catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa
    35881 tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg cgacaaagta
    35941 ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag
    36001 cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag ccaaatcgta
    36061 cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact tgagctcaag
    36121 ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa
    36181 cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat
    36241 cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa tggcgatcag
    36301 caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc
    36361 gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca gcaattctac
    36421 tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc
    36481 cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt
    36541 cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat
    36601 acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag gacttagcat
    36661 aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg
    36721 cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag cggcaatttt
    36781 aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga tgcccgttgc
    36841 aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt
    36901 accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga catgaatagc
    36961 ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg
    37021 taaggtaaaa atcagcgcaa tactgaccag agtcgccaac agccagccca gagtggtggc
    37081 aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac
    37141 tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc
    37201 tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg gaactgaaag
    37261 cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg
    37321 ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat caattactgg
    37381 cgattcactg taagcgcggt ctggtatggg gcgagctgga atttgtactg ccggacgaaa
    37441 aagtggtgcg tctgcacggt accgaatggg gcgagacgca gcgtttttac catcatcttg
    37501 atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc
    37561 agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa
    37621 cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccggttaacc
    37681 gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag
    37741 atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg cttaccgagt
    37801 atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag gcccgggcag
    37861 tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg
    37921 tgctggtggc ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct gagcaaattt
    37981 tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcgtatt cgcgaacggc
    38041 tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat attattcagc
    38101 agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct gcccgtcatg
    38161 aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct
    38221 ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg
    38281 atgaaaaatt acagcgtcgt cttgcctcac gcctcgatcg ttgggtaagt ctgatgcgga
    38341 tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt
    38401 tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt gcgctgaagg
    38461 cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt ctggagaaag
    38521 gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat atctcgccgc
    38581 agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg ttgttcgccg
    38641 ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg
    38701 ctttccatga aaactttggt gaaggcgaac gctgtgattt agacacgact taccgtttta
    38761 acagtcgtat cggtgaggtg gcaaaccggt ttattcagca gaacccaggc cagctgaaaa
    38821 agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg gatgagagtc
    38881 aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag cgcattctga
    38941 tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca acacgctggc
    39001 cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag gcggattacg
    39061 tcctcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg cgggagtcga
    39121 ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa gaacggcggt
    39181 taatgtacgt ggcgctgacc cgggcacgtc atcgggtatg ggcactgttt aacaaagaga
    39241 atccctctcc ctttgtggaa atactgaaaa atctggatgt gccggtggcg agaaaaccgt
    39301 aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg cgagataacg
    39361 ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact ggataatgaa
    39421 gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca gacgcagcaa
    39481 ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga aaatcaatac
    39541 atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca ttgggccact
    39601 caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt
    39661 tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc tcatcagcat
    39721 ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt
    39781 cgtcagttcc ataatgtaca tccgtagtta actttcctac agattactgt aagcacttat
    39841 cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca ggaagatggt
    39901 tgtttttccg cccattgcag gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa
    39961 tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc cagctcggaa
    40021 acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg
    40081 cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt
    40141 gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc atccaacaat
    40201 tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag gcgaggcagg
    40261 cgaaaattga cctggttgat gagctgggta ttaaaactga ccaccagcgg tggggagata
    40321 tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa cactaactga
    40381 tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc
    40441 ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg
    40501 gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat aggtagctcc
    40561 tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat acttactaac
    40621 atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat ggttggcatg
    40681 tcggctttgt cgtacacttt gtgaaacagt ctggaggaaa taatgaaaga aaccgatatt
    40741 gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga caaacccgat
    40801 cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt cattccggtt
    40861 tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtacggcac gctggcagac
    40921 gttccggaaa aagtcgatat ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgta
    40981 gcgcaggaag ctatcgccat tggcgcgaaa accctctgga tgcaactggg ggtgattaat
    41041 gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga tcgctgcccg
    41101 gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag gcaaaaacct
    41161 tccggggatc tgttcaggga ttagttacgc agacgcgggg cctggagttg tttgcggatg
    41221 gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa ttcgctactt
    41281 agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg
    41341 tgataccacg gagcggcgcg aagctcgtca ttcaccgcca gctcatcagg cgacggttca
    41401 gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa cagggaatgg
    41461 cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg ggaaatcttt
    41521 acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca ggcaaaccct
    41581 ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggccgc ctgacggaac
    41641 tgctctataa attgtacatc acgaccggca tcaattgcgg catccgcgat gattttctga
    41701 aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc gccttcattc
    41761 agcagctgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc catcaactgg
    41821 cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga
    41881 tcgcgatagg tacgcagcaa tttaaagaca tcatcacgga caaactcagc cttgctcaga
    41941 tccagtttgt tcagctcaac gttctgccgt gcaatatcca gtgcttcctg ggaggtatca
    42001 acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc accggtatag
    42061 gagaaacagt tcagtacgcg tttattttca acgtagcggc gggtagccag gcggctgtct
    42121 cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgta tgtccaccag cagcttcatg
    42181 ccatgttctt caatcggcag cagggcaggc ggcaactcac cggtgacgag gccctgggtc
    42241 agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat cgcacattcc
    42301 gggtacagcg tttgcagggc actaattaat gccgcgcgct gatattctgc gccagcactc
    42361 agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg caggccatca
    42421 gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag ccagtcacgc
    42481 catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg
    42541 tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc gcgtgctaac
    42601 cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt accttccata
    42661 cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt ttcgcgccct
    42721 ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa gaaatgggcg
    42781 tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag gagagcagca
    42841 atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc
    42901 tacaccacac agtacgaagc gaaaagactg ggcttaaccg ggtacgccaa aaatcttgac
    42961 gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtagaaaa attaatgcag
    43021 tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat
    43081 catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac cggttttggc
    43141 aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg gtataaatag
    43201 cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag catacgaatc
    43261 gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt
    43321 tctggcgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg ctcactccac
    43381 tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc ttcgaagatc
    43441 agcataatta ttactacctt aatcagactg ccggcagtgt aacaaaaaac aaagccccgc
    43501 ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc gaagcccaga
    43561 atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac cgtggcacga
    43621 atatagttcg tttcaccgcc atgaatgatg ttgctggttt caaacaaaat agcgccagag
    43681 gagatcaaaa tgaagaccgc gctgatcgcc agatgcagag caggcagctg caggaagata
    43741 ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc gccgaggaac
    43801 gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac taacgccgtt
    43861 ccgcccagtg ccatagcgat tacgtcaccc attccggcag acagataggt attcagaatt
    43921 ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat cccggtcggc
    43981 ttattcgcca ttttataggt caggaacatc aaaccataca tacccaccag cgtcagaatc
    44041 agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc
    44101 agcgtcaggc tcagcagaaa ataggtatta cgcagcactt tatgggtgct aagcagtgat
    44161 gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat gacagatgta
    44221 attaattaag cagcataatg ataatgcgta agggcaccca gtagttttac ccatctttac
    44281 gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa atataacgcc
    44341 ctgagaattt cggcaggcaa aagaaaaagg ggttagcatt tagctaaccc cttatcttat
    44401 ctggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc
    44461 gaagaaaaac aaataaaaat cagtgtaata cgtgaaattg ctgaaaagtt aatcaaattt
    44521 aacaccaaga aaaatcaagg tattaggaag ttgctcatag ctagtatacc aagtcttttt
    44581 gccagaaatt tttctggatt gtctacttgc taaccacgag cggcaaatcc agtgttggtg
    44641 tgatttttgt tttacgatcg taaatcaaca cctgattttc tgttttatgc ccgctgaaaa
    44701 tttgtttgtc gcgactgctg ccttcgtaat ctgagatccc cttggctttt atgtcgtgaa
    44761 aattgcaccc gaacggaacg ccaacttttt gctcggctgc gcgtttagcc tgattccacc
    44821 agttattcag cgttttagct atgactttcc cgcctttggt tgtattgatc acatattcgc
    44881 atgtacagga agatacattt cgggctaact ggatcgctgt acgtaatcgc ggtgaccatt
    44941 ccttgatttg tttggtgccg gttttgtttt gctcaatgta gatcccttta tccataatat
    45001 cctgccattt caactcaagc acatcaccga gtcttgccgc acagagatag gaaatctcca
    45061 ttgcaatgcg taactgtgga attgcttccg catatatcgc cgcatactgt tcatcggtga
    45121 tgtaaacagt gcgggcttta agagagaatt ttctgactcc tttgcatggg ttattcttca
    45181 catacccacg ctcatatccc cacccgtata ctcgactcag gcttgccagt tcatgatttg
    45241 cccaagccct cgtttatcca tgaaaattct tacctgctca gttttgacat tatcagcaag
    45301 cacttttccg aataccatca gcaacgcctt ctgatgttgt cgataatctt tttgggttcg
    45361 gggggccagt tctgtaaatg caggggagtc cataaacatg tgccacaatt tagctacggt
    45421 cattatgttg tggagttttg ctttttccag ttcataattt tgccagactt tagctacgct
    45481 ggttttccgc actcttccta gccctatact tcttgtactt ccttcgggtt tccacacgta
    45541 actgtaacca ttcgatctaa cccgcggtgg cagtacatta tcttttttat tttttcttgg
    45601 tcttcccatt gttcagcgcc tcaaaatcgg gttcagcaga aaccagttca gatgtttttg
    45661 gcatcgttgt cagaccgtgt ggaatatccc tgcggagaac tatgggttcg tttttagggc
    45721 cgattacaaa tgggatgccg tgcagtctta actggtgttg ctgttttgtg tatcgctcgt
    45781 atttcgtgat ctctttaatc tctgctggag atagagttaa ttcgtacatg tggtcacgtg
    45841 cctttacagc atgaccgccg ccactatagc tggtgggcgg cgatatgggt tgaacatcaa
    45901 aaatcagcct gactcgggag cagtttttgc cagatggctg aaacgtattt cgcctggtaa
    45961 cg
//
LOCUS       DBFIFS010000010        45738 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.010, whole genome shotgun sequence.
ACCESSION   DBFIFS010000010 DBFIFS010000000
VERSION     DBFIFS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.010"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(413..499)
                     /locus_tag="N7A07_000565"
     tRNA            complement(413..499)
                     /locus_tag="N7A07_000565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Leu,seq:gag)
     gene            complement(514..846)
                     /gene="secG"
                     /locus_tag="N7A07_000566"
     CDS             complement(514..846)
                     /gene="secG"
                     /locus_tag="N7A07_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU2475294.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1074..2411)
                     /gene="glmM"
                     /locus_tag="N7A07_000567"
     CDS             complement(1074..2411)
                     /gene="glmM"
                     /locus_tag="N7A07_000567"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU2475295.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2404..3252)
                     /gene="folP"
                     /locus_tag="N7A07_000568"
     CDS             complement(2404..3252)
                     /gene="folP"
                     /locus_tag="N7A07_000568"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU2475296.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3342..5276)
                     /gene="ftsH"
                     /locus_tag="N7A07_000569"
     CDS             complement(3342..5276)
                     /gene="ftsH"
                     /locus_tag="N7A07_000569"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU2475297.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5376..6005)
                     /gene="rlmE"
                     /locus_tag="N7A07_000570"
     CDS             complement(5376..6005)
                     /gene="rlmE"
                     /locus_tag="N7A07_000570"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU2475298.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6131..6424
                     /gene="yhbY"
                     /locus_tag="N7A07_000571"
     CDS             6131..6424
                     /gene="yhbY"
                     /locus_tag="N7A07_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU2475299.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6580..7056)
                     /gene="greA"
                     /locus_tag="N7A07_000572"
     CDS             complement(6580..7056)
                     /gene="greA"
                     /locus_tag="N7A07_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU2475300.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7304..8737
                     /gene="dacB"
                     /locus_tag="N7A07_000573"
     CDS             7304..8737
                     /gene="dacB"
                     /locus_tag="N7A07_000573"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU2475301.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8777..9949)
                     /gene="cgtA"
                     /locus_tag="N7A07_000574"
     CDS             complement(8777..9949)
                     /gene="cgtA"
                     /locus_tag="N7A07_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU2475302.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9965..10930)
                     /gene="yhbE"
                     /locus_tag="N7A07_000575"
     CDS             complement(9965..10930)
                     /gene="yhbE"
                     /locus_tag="N7A07_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2475303.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLKSLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11057..11314)
                     /gene="rpmA"
                     /locus_tag="N7A07_000576"
     CDS             complement(11057..11314)
                     /gene="rpmA"
                     /locus_tag="N7A07_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU2475304.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11335..11646)
                     /gene="rplU"
                     /locus_tag="N7A07_000577"
     CDS             complement(11335..11646)
                     /gene="rplU"
                     /locus_tag="N7A07_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU2475305.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11905..12876
                     /gene="ispB"
                     /locus_tag="N7A07_000578"
     CDS             11905..12876
                     /gene="ispB"
                     /locus_tag="N7A07_000578"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU2475306.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13105..13383
                     /gene="sfsB"
                     /locus_tag="N7A07_000579"
     CDS             13105..13383
                     /gene="sfsB"
                     /locus_tag="N7A07_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU2475307.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13431..14690)
                     /gene="murA"
                     /locus_tag="N7A07_000580"
     CDS             complement(13431..14690)
                     /gene="murA"
                     /locus_tag="N7A07_000580"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU2475308.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14745..14999)
                     /gene="ibaG"
                     /locus_tag="N7A07_000581"
     CDS             complement(14745..14999)
                     /gene="ibaG"
                     /locus_tag="N7A07_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU2475309.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15159..15452)
                     /gene="mlaB"
                     /locus_tag="N7A07_000582"
     CDS             complement(15159..15452)
                     /gene="mlaB"
                     /locus_tag="N7A07_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU2475310.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15452..16087)
                     /gene="mlaC"
                     /locus_tag="N7A07_000583"
     CDS             complement(15452..16087)
                     /gene="mlaC"
                     /locus_tag="N7A07_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU2475311.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16106..16657)
                     /gene="mlaD"
                     /locus_tag="N7A07_000584"
     CDS             complement(16106..16657)
                     /gene="mlaD"
                     /locus_tag="N7A07_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU2475312.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16662..17444)
                     /gene="mlaE"
                     /locus_tag="N7A07_000585"
     CDS             complement(16662..17444)
                     /gene="mlaE"
                     /locus_tag="N7A07_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU2475313.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17452..18261)
                     /gene="mlaF"
                     /locus_tag="N7A07_000586"
     CDS             complement(17452..18261)
                     /gene="mlaF"
                     /locus_tag="N7A07_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU2475314.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18471..19448
                     /gene="yrbG"
                     /locus_tag="N7A07_000587"
     CDS             18471..19448
                     /gene="yrbG"
                     /locus_tag="N7A07_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU2475315.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19462..20448
                     /gene="kdsD"
                     /locus_tag="N7A07_000588"
     CDS             19462..20448
                     /gene="kdsD"
                     /locus_tag="N7A07_000588"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU2475316.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20469..21035
                     /gene="kdsC"
                     /locus_tag="N7A07_000589"
     CDS             20469..21035
                     /gene="kdsC"
                     /locus_tag="N7A07_000589"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU2475317.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21032..21607
                     /gene="lptC"
                     /locus_tag="N7A07_000590"
     CDS             21032..21607
                     /gene="lptC"
                     /locus_tag="N7A07_000590"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU2475318.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21576..22133
                     /gene="lptA"
                     /locus_tag="N7A07_000591"
     CDS             21576..22133
                     /gene="lptA"
                     /locus_tag="N7A07_000591"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU2475319.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22140..22865
                     /gene="lptB"
                     /locus_tag="N7A07_000592"
     CDS             22140..22865
                     /gene="lptB"
                     /locus_tag="N7A07_000592"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU2475320.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22913..24346
                     /gene="rpoN"
                     /locus_tag="N7A07_000593"
     CDS             22913..24346
                     /gene="rpoN"
                     /locus_tag="N7A07_000593"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU2475321.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24369..24656
                     /gene="hpf"
                     /locus_tag="N7A07_000594"
     CDS             24369..24656
                     /gene="hpf"
                     /locus_tag="N7A07_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU2475322.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24774..25265
                     /gene="ptsN"
                     /locus_tag="N7A07_000595"
     CDS             24774..25265
                     /gene="ptsN"
                     /locus_tag="N7A07_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU2475323.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            25311..26165
                     /gene="rapZ"
                     /locus_tag="N7A07_000596"
     CDS             25311..26165
                     /gene="rapZ"
                     /locus_tag="N7A07_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU2475324.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26162..26434
                     /gene="npr"
                     /locus_tag="N7A07_000597"
     CDS             26162..26434
                     /gene="npr"
                     /locus_tag="N7A07_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU2475325.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26648..27280
                     /gene="yrbL"
                     /locus_tag="N7A07_000598"
     CDS             26648..27280
                     /gene="yrbL"
                     /locus_tag="N7A07_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU2475326.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(27277..28005)
                     /gene="mtgA"
                     /locus_tag="N7A07_000599"
     CDS             complement(27277..28005)
                     /gene="mtgA"
                     /locus_tag="N7A07_000599"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU2475327.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28002..28655)
                     /gene="elbB"
                     /locus_tag="N7A07_000600"
     CDS             complement(28002..28655)
                     /gene="elbB"
                     /locus_tag="N7A07_000600"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU2475328.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28885..31221)
                     /gene="arcB"
                     /locus_tag="N7A07_000601"
     CDS             complement(28885..31221)
                     /gene="arcB"
                     /locus_tag="N7A07_000601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU2475329.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31317..32246)
                     /gene="yhcC"
                     /locus_tag="N7A07_000602"
     CDS             complement(31317..32246)
                     /gene="yhcC"
                     /locus_tag="N7A07_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU2475330.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            32920..37380
                     /gene="gltB"
                     /locus_tag="N7A07_000603"
     CDS             32920..37380
                     /gene="gltB"
                     /locus_tag="N7A07_000603"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU2475331.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37393..38811
                     /gene="gltD"
                     /locus_tag="N7A07_000604"
     CDS             37393..38811
                     /gene="gltD"
                     /locus_tag="N7A07_000604"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU2475332.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38995..40044
                     /gene="yhcG"
                     /locus_tag="N7A07_000605"
     CDS             38995..40044
                     /gene="yhcG"
                     /locus_tag="N7A07_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU2475333.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(40104..40568)
                     /gene="nanQ"
                     /locus_tag="N7A07_000606"
     CDS             complement(40104..40568)
                     /gene="nanQ"
                     /locus_tag="N7A07_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU2475334.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40565..41440)
                     /gene="nanK"
                     /locus_tag="N7A07_000607"
     CDS             complement(40565..41440)
                     /gene="nanK"
                     /locus_tag="N7A07_000607"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU2475335.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41437..42126)
                     /gene="nanE"
                     /locus_tag="N7A07_000608"
     CDS             complement(41437..42126)
                     /gene="nanE"
                     /locus_tag="N7A07_000608"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU2475336.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42174..43664)
                     /gene="nanT"
                     /locus_tag="N7A07_000609"
     CDS             complement(42174..43664)
                     /gene="nanT"
                     /locus_tag="N7A07_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU2475337.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43773..44666)
                     /gene="nanA"
                     /locus_tag="N7A07_000610"
     CDS             complement(43773..44666)
                     /gene="nanA"
                     /locus_tag="N7A07_000610"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU2475338.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44788..45579)
                     /gene="nanR"
                     /locus_tag="N7A07_000611"
     CDS             complement(44788..45579)
                     /gene="nanR"
                     /locus_tag="N7A07_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU2475339.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(45557..>45738)
                     /locus_tag="N7A07_000612"
     CDS             complement(45557..>45738)
                     /locus_tag="N7A07_000612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475340.1"
                     /translation="MFLSALHYFFAIVNYSVNYGNDEAQRENPAIFPYFQSCDRMSLM
                     MLITLTLQNGPYERI"
BASE COUNT        11247 a        12447 c        11385 g        10659 t
ORIGIN      
        1 gcaattttat atcagacatg tttttcaact ctgatccata tcagaacgaa acactgttag
       61 ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg
      121 cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg
      181 tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg
      241 ccaattatgt cagcagtagg gaaaagttta taacgaaggc gtaccggcac catcgtttca
      301 aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca
      361 gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg
      421 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
      481 ccaattccac cacctcggca cggaaactac tttttagttc gggatatcgc tggtcggctt
      541 agccggagca gctggctgag tttgttcggt tttcgccggc gcactcaggt tttcccattc
      601 gctaccttta ttggtcttgt tgctattgat gttacccagc accagactga tgatgaagaa
      661 taacgttgcc agcaacgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
      721 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
      781 tagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc
      841 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
      901 gggagtttat aatctttccc actgaagcgg gtgtgaatac taaccaaaac gaatgacctt
      961 cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
     1021 tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg
     1081 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
     1141 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg
     1201 ctacccagcg ccgtttcaac ttctgcggtc accgctttga ctgattcatg ctccagcgga
     1261 tcgccgctac ctgcggtgta acgcacgttt accaggattt gcgggaacat tttcatgccg
     1321 ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac ctgcaaacca
     1381 gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct
     1441 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
     1501 gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc
     1561 aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
     1621 tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg
     1681 ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
     1741 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg
     1801 atagcgataa cattcgctcc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca
     1861 ccgtttgcgc aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg
     1921 gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt
     1981 tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc ttcttcaact
     2041 gcatccggca gcttagtacc gtcaatagaa aagaatttaa tgccattatc gtagaacggg
     2101 ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc
     2161 acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgcagc cgccagcccc
     2221 gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc
     2281 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
     2341 tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
     2401 cgattactca tagcgtttgt tttcctttgc agacagagta gcttccacca ctcgcatcgc
     2461 ttctacggtt tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat
     2521 gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc
     2581 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
     2641 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
     2701 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
     2761 gtagcgattc acttctgcaa agacatcgtc atacttcggg gcttcctgca tggtttttgg
     2821 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc
     2881 gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc
     2941 acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat
     3001 tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc
     3061 tggccgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt
     3121 cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg
     3181 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc
     3241 aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
     3301 ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc
     3361 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
     3421 ggactaccat tgtcgccagc attgttagaa gcgcctggtt cttcccagcc cgctggcgga
     3481 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
     3541 atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca
     3601 cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
     3661 tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc
     3721 gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat gttacgtgcc
     3781 aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag
     3841 atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga
     3901 cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
     3961 cgtgggataa tcgtcacttt gtgcaccgga tcgtgctccg gcaccaggcg accgataatc
     4021 gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag
     4081 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca
     4141 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
     4201 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat
     4261 ggtacgcgac gcatgtgaac cttcaggatc tgctcacgac cgcgaacatc tggcaagcca
     4321 accacaacct gacggtcgaa acggccagga cgcagcagcg ctgggtcgag aacgtccgga
     4381 cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc
     4441 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc tgcgccacgc
     4501 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc
     4561 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
     4621 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc
     4681 gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt
     4741 ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc ttcttttgct
     4801 tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc
     4861 gctttactct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
     4921 aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc
     4981 agcaggctgg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta
     5041 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
     5101 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
     5161 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
     5221 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
     5281 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
     5341 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
     5401 atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta
     5461 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
     5521 acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga
     5581 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
     5641 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
     5701 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
     5761 caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc
     5821 ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
     5881 atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca
     5941 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
     6001 gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
     6061 ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc
     6121 aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc
     6181 gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat
     6241 tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga
     6301 aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt
     6361 catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg
     6421 ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca
     6481 ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gctatgcggc ctttttcctt
     6541 tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat
     6601 tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt
     6661 gccgatcagg ccacgagcaa taggtgagtt tacagaaatc aggttttgtt taaagtcagc
     6721 ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatcgagat tcagcaccgt
     6781 tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg
     6841 cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc
     6901 acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat
     6961 agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt
     7021 ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct
     7081 gatggggcaa gtgttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg
     7141 gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt
     7201 ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc
     7261 gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt
     7321 tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta
     7381 cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc
     7441 ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta cccagaaagt
     7501 gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct
     7561 tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg
     7621 tgccgatccg acgttaaaac gtcaagatat tcgcaatatg gtcgcgactt tgaaaaaatc
     7681 tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga
     7741 taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgt
     7801 cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga
     7861 tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac
     7921 cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtggtgc caggcgacct
     7981 gaaccgcttt acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt
     8041 tgccgtgcag gatggagcca gctatgccgg tgcaattctg aaagatgagt taaaacaggc
     8101 gggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt
     8161 agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc
     8221 ggacaacatg atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc
     8281 tggaacatgg cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt
     8341 cgatattgga aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc
     8401 ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt
     8461 tatctccatg ctgccgctag cgggctatga cggctctttg cagtaccgtg caggtctgca
     8521 tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa
     8581 cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc
     8641 tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga
     8701 aagccgtttg tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg
     8761 gccgggtttt tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt
     8821 cccagtcgtc atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat
     8881 gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga
     8941 ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac
     9001 tcgccgcaga gatcagataa tatttatctt cccagcccag cgcctctgcg atcgctttcg
     9061 ctttctcttc ggcttctacc ttatccagca gatcgatctt gttgaagact aaccaacgcg
     9121 gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt
     9181 caaccggatc ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac
     9241 gttccaggtg cttcaggaag cgaatgccca gacctgcgcc ttccgcagcg ccttcaatca
     9301 gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac
     9361 ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcac
     9421 gaataaaggt cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca
     9481 tcagctccag cagcagctca cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg
     9541 tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa
     9601 ccagcagacg ctgaccgtgt ttggtcatat cgcccatggt ttcaccagta ccctggtcga
     9661 ttacacgcgt acctaccggc actttaattg tgacgtcttt accgcgctta ccggtacagt
     9721 cgcggcttgc gccattctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga
     9781 taagcgtgtt caggttctcg tcggcttcca tccatacgtc accaccatca ccgccgtcgc
     9841 cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac
     9901 cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg
     9961 taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc
    10021 aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat
    10081 aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat
    10141 ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct
    10201 gccagcgagc catcgcttcc gccagggcgc catatcctac caaggtattc agtccgcaaa
    10261 aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag
    10321 ggaagagcgc aattgtacat aaagtgtaca gtaaaaacag gatctgcggt gaggccagcc
    10381 gacgcaataa aaccttttgc gccacgccat aactcaccca aaccgtcgca gcaccgaccc
    10441 caaagataac tccccaggtg taatcggtta gctttgtaaa tatctcgacc agactggtgt
    10501 taaaaaacat caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca
    10561 ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa
    10621 tcacctgcga agcggtcgga ctcagggatt tcaaggatga gctgaacagg atgaagttcc
    10681 caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc
    10741 gtaatggcgg caacctcttc ttcaccgcaa gaatggcccc caggccaata ctcgccatca
    10801 agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcaa
    10861 ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct
    10921 gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca
    10981 tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta
    11041 ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt
    11101 taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac
    11161 ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca
    11221 gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt
    11281 tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc
    11341 gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata
    11401 gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac
    11461 ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt
    11521 tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc
    11581 caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc
    11641 gtacatataa aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat
    11701 attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa
    11761 tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta
    11821 caatcaccct atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc
    11881 ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa
    11941 gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccaactgatc
    12001 aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct
    12061 gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg
    12121 atcgagttta tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg
    12181 cgcaggggta aagctaccgc caacgccgca tttggcaatg ccgccagcgt gctggtaggc
    12241 gattttattt atacccgcgc gttccagatg atgaccagcc tcggttcact caaagtgctg
    12301 gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaat
    12361 gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg
    12421 cgtctgtttg aggctgccgc gcagtgttcc ggaattctgg cgggctgtac gccggaggag
    12481 gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat
    12541 ttactcgatt acaatgccga tggcgaacag ttaggtaaaa acgtcggcga cgatctgaac
    12601 gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca
    12661 cagatgatcc gtaccgccat cgaacagggt aacggtcgcc atcttctgaa accggttctg
    12721 gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgcgccga ggaagaagca
    12781 gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc
    12841 ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagt
    12901 gcgcgggatg attccagtaa actccataag aatacctatt cagcgtaata atgcactgaa
    12961 aaaccagtgc atacatcttt atatattctg aatattcaca ctctttatag gaacttttta
    13021 gagcaatcgg ccatcaggag tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc
    13081 gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca tcccgctgac
    13141 atcattgcgg gtttgcgcaa gaagggaaca tcaatggcgg cggagtctcg cagaaatggt
    13201 ttgagttcct caacgctggc gaatgcatta tcgcgcccat ggccgaaagg agagatgatt
    13261 attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta ccatgatccg
    13321 cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa accgaaaaaa
    13381 tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga ttattcgcct
    13441 ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg ttcgtatcca
    13501 cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca gccagccagc
    13561 accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga aagtttttca
    13621 acaccgtgac aaataacggt attgctttcg atttcggcgt gcgcgcccat acggctcagc
    13681 tctggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc agtcccttct
    13741 gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc
    13801 gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct aatccagtct
    13861 tcgccgacct cgatgtccgc tccagcgtca cgcagtttcg ccagcacggc gtcgagagta
    13921 tctggctgcg tgttacggca gataattttg ccgcgagaaa tcgccgccgc caccaggaaa
    13981 gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa acgttccaca
    14041 ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag cgtaatcagg
    14101 aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat cgtggtgcct
    14161 tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac tttatccatc
    14221 acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc ttcttccagt
    14281 ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg acgcgcaccg
    14341 atcgtacaac cgccaggtag tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc
    14401 agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca gaatacatta
    14461 acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc cagctggctt
    14521 agcagcttca ttgatgtatc gacgtctttc agtttcggga cgttctggat ctctaccggt
    14581 tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc gccggaaatt
    14641 gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat ttagtttgtt
    14701 ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa gccgttcagt
    14761 ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc atgaatgcgg
    14821 ttatccgcaa tatattccat cagcggacca tagaccgtct gctgtttttt aacccgactc
    14881 atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc gccggaaacg
    14941 tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt attttccatg
    15001 ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt tgcgctggca
    15061 taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag gcaggtttgc
    15121 cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag aacatcagca
    15181 ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg aagcgtcacg
    15241 ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag tccacccgta
    15301 tccacgcggg acacacggct aagatcgatg caggtaatcc ccttcaccgc ttcctcacgc
    15361 atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc cagtgtgtca
    15421 cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga gtgattttct
    15481 gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat acctttggta cgtagcagcg
    15541 ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag
    15601 cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc accggcgggc
    15661 ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatca cccagcggct
    15721 gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct
    15781 gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg ttgagctggg gtcgcactct
    15841 tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt
    15901 cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt tgctcattct
    15961 tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga ttggtctggt
    16021 ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt
    16081 taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca gtggtttcat
    16141 tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg cctttactac
    16201 cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttggtg tcctgaattg
    16261 tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg
    16321 ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg gtatcaggaa
    16381 tatggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt ttcgggtcca
    16441 gcgtaatatc cgccacccgg cccacaacaa cgccaccaat actgaccgga gagcgcgctt
    16501 tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca gtacgtattg
    16561 acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagcagtgct gctaataaaa
    16621 agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc aaacatcaat
    16681 gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac ggtgcgagtg
    16741 gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa caacgaaatc
    16801 cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag atccatacgc
    16861 cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc tttccaactg
    16921 acgccgacca gcgatccgcc ccagatcccc acggcgacga agataaccgt caacagtggt
    16981 aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg atccaccgcc
    17041 atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc tgcggttagc
    17101 gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag ttcacgcagt
    17161 agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt cagaaccaga
    17221 taaccttgca gccccaacac cattccgatg aacacgccag aaactacaat aatcagcatc
    17281 gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt gcgaaattcc
    17341 ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccaaacgt tctcagggtt
    17401 ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg cttaactccc
    17461 tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaaccg gcccgtcagc
    17521 tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg cctgggcact
    17581 gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatccgcaa tacttaacac
    17641 ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt tcagctcaga
    17701 aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag gttcatcaaa
    17761 catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc
    17821 accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg cctccagctt
    17881 catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc gcagtggata
    17941 ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa ataacatgct
    18001 catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat tctcaccatc
    18061 gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt
    18121 tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga
    18181 aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca tatcgactaa
    18241 attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc taagcatggc
    18301 gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg gttagcgaaa
    18361 gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt
    18421 tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag atgcttttag
    18481 ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac cgcctggttt
    18541 ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc ggcatgacgg
    18601 tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg
    18661 aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat atattgctga
    18721 tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttccgatgtt ctacgccgtg
    18781 aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat gacggacaac
    18841 ttagtcgcag cgatggtatc tttctcctct ttctggctgt gctatggctg ctgttcattg
    18901 ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga gagcagcttg
    18961 cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc attgcgctta
    19021 tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg gcgaattact
    19081 ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc agcctgccgg
    19141 aactggcaac cgcaatagcg ggcgttcgca aaggtgaaaa cgacattgct gtcggaaata
    19201 tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg ctgataacgc
    19261 caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg ctggtgagca
    19321 ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt gtaggggtat
    19381 tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg ccaatactcg
    19441 ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt tgactttcag
    19501 caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac
    19561 atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc
    19621 gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac gtttgccagc
    19681 accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga tttaggcatg
    19741 gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag cgaaatcact
    19801 gccttaattc cagtgcttaa gcgtcttcac ataccgttaa tctgcatcac cggtcgcccg
    19861 gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc
    19921 tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat gggcgatgcc
    19981 ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc gctctcacac
    20041 ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat gcatacgggc
    20101 gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct ggaagttacc
    20161 cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga aggcatcttt
    20221 accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt
    20281 gccgatgtga tgacgccggg gggaatacgc gtgcgccctg gcattctggc cgttgaggca
    20341 ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg cgaccattta
    20401 ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag attcaaggat
    20461 aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg
    20521 acgttatagc aaaagcagag aacattcgtc tgctaatcct cgacgttgat ggcgtactgt
    20581 cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg
    20641 acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc attaccgggc
    20701 gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac ttgtatcagg
    20761 ggcagtcaaa caaactgatc gcctttagcg atctgctgga aaaattggcg attgccccgg
    20821 aaaatgtggc ttatgtcggc gatgatctca tcgattggcc ggtaatggaa aaagtgggtt
    20881 taagcgtcgc cgtggccgat gcgcatccac tgttgatccc gcgcgccgat tacgtgacgc
    20941 gcattgctgg cggtcgtggc gcagtgcgag aagtttgcga cttattactc ctggcgcagg
    21001 gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt gggttatcat
    21061 tgtgctatca ctggcggttc tggtgatgat cggtattaat atggccgaaa aagacgatac
    21121 cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata cggacacgct
    21181 cgtctataac ccagaagggg cgctaagcta tcgattgatt gctcaacacg ttgaatatta
    21241 ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg ataaggataa
    21301 aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc ggatgctcta
    21361 tttatatgga cacgttgaag tcaacgcact tgtgccggac tctcaacttc gcagaatcac
    21421 gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag acctcgtcac
    21481 gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact tacgcagcaa
    21541 gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca aacaaactca
    21601 gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca tttgccgtaa
    21661 ccggagacac tgatcagccg atacacattg aatcggacca gcaatctctt gatatgcaag
    21721 gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccata aaaattaatg
    21781 ccgacaaagt ggtcgttacc cgtccgggtg gcgaacaagg taaagaagtg attgacggct
    21841 acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt gaaggtcacg
    21901 cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt aatgcttatc
    21961 tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggtg aaagagcaga
    22021 aaatgcaggc tttcagcgac aaaggcaagc gtgtaaccac cgttctggtg ccgtcgcagc
    22081 tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat taattcgtta
    22141 tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc gtggtagaag
    22201 acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca aacggtgccg
    22261 gtaagaccac cactttctac atggttgtag gcattgtgcc gcgcgatgcg ggcaacatca
    22321 ttattgatga tgacgatatc agtctgctac ctctgcatgc acgcgcgcgc cgcggtatcg
    22381 gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat aacctgatgg
    22441 cggtactgca aattcgtgac gacttgtctg ctgaacagcg tgaagaccgc gcgaacgagc
    22501 tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca ctctccgggg
    22561 gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa tttattctgc
    22621 tcgacgaacc gtttgccggg gttgacccga tctcagttat cgacattaaa cgcatcattg
    22681 agcacctgcg cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg cgtgaaacac
    22741 tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc cacggcacgc
    22801 ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa gacttcagac
    22861 tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga acatgaagca
    22921 aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc aacaggcaat
    22981 tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg cgctggagag
    23041 taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg aaacgcaaga
    23101 cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc
    23161 gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca ccagcggtga
    23221 ctacattgac gacgagctgc cggtctatca gggcgaaacg acgcagacct tgcaggatta
    23281 cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta ttgctacctc
    23341 tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag atattctcga
    23401 aagtatgggc gatgaagaga tcgacatcga cgaagttgaa gccgtcctta agcggatcca
    23461 acggtttgat ccggtcggtg tggcggcaaa agatctgcgt gactgcctgc tgatccaact
    23521 ctcccaattc gataaaacca cgccgtggct ggaagaggcc agactgatca ttagcgatca
    23581 tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc gtctgaaaga
    23641 agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc ccgggcaatc
    23701 gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta agcataacgg
    23761 tcactggacg gtagaactca acagtgacag cattcctcgt ctgcaaatca accagcacta
    23821 cgcctcgatg tgcaataacg cgcgcaatga tggtgacagc cagtttatcc gcagcaatct
    23881 gcaagatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt
    23941 gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag aatatatgaa
    24001 accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg
    24061 cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga agtatttctt
    24121 ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct
    24181 ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac
    24241 ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga agtaccgaga
    24301 gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca accgataagg
    24361 aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag gcactgcgcg
    24421 aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc aaccaggtct
    24481 atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg catgtaaacg
    24541 gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt gatggcttaa
    24601 ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa cactaattgt
    24661 ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca tttgtacggt
    24721 taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa attatgacaa
    24781 ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg
    24841 tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac
    24901 ttagcctacc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa atgggcagta
    24961 ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat actctgcgtg
    25021 ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc gacaaccaac
    25081 cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg cacctgcata
    25141 ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag
    25201 cccagagcga tgaagagctg tatcaaatca ttacggatac caaaggtact ccggatgaag
    25261 cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac atggtactga
    25321 tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata
    25381 tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg gctcgaaccc
    25441 tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg ccggagtcac
    25501 cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg caactactgt
    25561 tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc cgactgcatc
    25621 cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc gatctgctgg
    25681 agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc gttcacgagc
    25741 tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg acaatggtct
    25801 ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc tttgacgtgc
    25861 gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt cttgataagc
    25921 ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac cagacgcgaa
    25981 gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac ttgacggtcg
    26041 ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa ctggcagact
    26101 acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa aaacgtaaac
    26161 catgactgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc
    26221 aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac gtaatgacga
    26281 aggcaccgag gctgaagcca acagcgttat tgcgctgctg atgttggatt ctgccaaagg
    26341 acggcagatt gaagttgaag cgaccggtcc acaggaagag gaagcactgg ccgccgttat
    26401 cgccctcttt aattctggtt ttgatgaaga ttaatcttca tcactttgac atacaactcc
    26461 cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca atcaacgtca
    26521 attgtacatt tcgtgcgctt tttactttca gaagaacctt aagaaaacct taagaagcat
    26581 tgtttaggtt tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg
    26641 agatggcatg attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg
    26701 ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg
    26761 tggcgataaa gagatccgtc gtgagttaaa gtactacgcg catcttggtc gccggttaaa
    26821 agactggagt ggaataccgc gctatcacgg taccgtcgag acggattgcg gaaccggtta
    26881 tgtttatgat gtgattgctg attttgacgg caagccctcc attacgctga ccgaatttgc
    26941 tgaacaatgc cgttacgaag aagacgttgc acaactgcgc caattactga aacagctgaa
    27001 gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg
    27061 tcaccgcatt agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagcac
    27121 attgattcca ctggcgacct ggtcaaagtg gtgttgtctg cgtaagcaag agcggttgtg
    27181 gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg
    27241 tgagagcaaa acgctggccc tcgcttcgcg cgaagcttaa tccagctggt gttgttgcat
    27301 aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctgcgcac
    27361 gtagcccgat ggcgcggaga ctttgaaacg aagtggatta ggtaatacag ctgccagtaa
    27421 ggcagcttcc gatcgggtaa gtttgctcgc gggtttgtgg aaataacgtt gtgccgcagc
    27481 ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg
    27541 ctttttgctc cagacagttt ctatccccag cgttaatccg gcttccagcc cttttcgaac
    27601 ccagctacgc ccatcccata aaaagagatt tttggctgtc tgttgagaaa tcgttgaagc
    27661 accgcgaata cggttttcat tgcgctcatt gtgcgccagg gctttctcaa tggaagcgac
    27721 atcaaagccc cagtgctcag gaaatgtctg atcttctgcg gcaatcaccg ccagtcccat
    27781 ccacggcgag atttgatcca tactgaccct gtcagaatgt gccacgtaac gaaaattacc
    27841 atgcagccag gcgctgacct gtcgctcgac cattaccgca gagaagggaa caggtgcaac
    27901 gctaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcagaag
    27961 aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc
    28021 acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata
    28081 tatgccgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacac
    28141 ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg
    28201 gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa
    28261 ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg
    28321 gtgcattcgc gaccaagact ggcaaaattg cttaaattct ttgccgcgcc aaaccccccc
    28381 ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg
    28441 cgcgttattc gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc ttcgccagtt
    28501 aaatgattga taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg
    28561 ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg
    28621 ccgcatccgc tcagaattac gccaattttc ttcattatga tcatcctttc gcaattgact
    28681 gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact gattcatgta
    28741 acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgcagtgccc
    28801 ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat
    28861 tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag
    28921 cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc
    28981 ttcccaggcg ggaaggtcag gagactgaat ttgctgaccc agctgttgca gatggcgcaa
    29041 ccccactgac cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc
    29101 ctgcgccgtc agattcgact ccagcacgtt gacatagcct ggcatcatct tctcaaacac
    29161 cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg
    29221 aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc
    29281 atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaacag aaagcggctt
    29341 actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca gcacgttagc
    29401 ggttaaggcc accagcggcg gtaaatcctc tcgcggataa cgcttcgtca gttcacgaga
    29461 gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc
    29521 cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa
    29581 tttttccagc acagaacgcg cgacaatcac gttcagttca atgtcttcca ccagcagcac
    29641 attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgctac
    29701 cgacggtgcg tggatcgtca acgtaaaggt tgaaccttta ccctgttcgc tggtaaccgt
    29761 aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc
    29821 ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga agattttatc
    29881 cagctcatcc tgcggaatgc cgataccaga atcttccact tcaaaatgca gcatatcgcc
    29941 ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact
    30001 gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa
    30061 tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca atgcggagag
    30121 attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa gctggacctt
    30181 gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca gcgtgacggc
    30241 cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg tatccagcag
    30301 aatgcggctc aggccgacga taccgttcag cggtgtacgc aattcgtggc tgatggtgga
    30361 aataaacgtc gttttgtcgc ggctggcgcg ttcaagcgca tcctgatacc gcttacgctc
    30421 ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg
    30481 cactttacga atttcaaagc aggctttgcg tccgtcggga taatccagcc actgctcata
    30541 ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt tcgcagcggc
    30601 ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc tttttccggt
    30661 cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac
    30721 caggtcgggt gaagcatcaa ggaaggaacg taaaaatgag gattgctgct cgagctgaat
    30781 ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc
    30841 tgcttccgct ttttcacgaa cggcaatttc ctgatttagc tgggcaatat tatcttttaa
    30901 ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga
    30961 cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca
    31021 cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc
    31081 atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc
    31141 cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc
    31201 cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc
    31261 aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc aacgacctac
    31321 tccgttggag gtagccacgg acgtcccagc gccgatccct gtacgccatg ttcattcaga
    31381 tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagagg agcaagcagc
    31441 gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga
    31501 atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa acgccccgct
    31561 tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag ctttatgccg
    31621 tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg cccttcgcca
    31681 ggcaggccga caattaagtg agaacacacc ttcagcccgc gctgacgtgc cagctgggtt
    31741 gtacgctgat aacaggcaaa atcatggccg cgattaatgc gatgcagggt tttgtcgtgg
    31801 gcggtttgta gccccagctc cagccacact tcgtagccct ggtccttata ttcgcaaagc
    31861 agatccagca ccgcatccgg cacgcagtcc gggcgagtac caacacacaa accgacaata
    31921 ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg
    31981 ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg
    32041 tgcgccagtt gctcggcaat ggaacgatgt tgctgcgctt catcggcaaa tgaggcaaca
    32101 ttacagaatg tgcagccgcc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg
    32161 ccgtgcagcg tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac
    32221 atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa
    32281 gcctgccatt tttagctcaa ttcggcgatg acttggatca atcgtcccgg cctgctttta
    32341 tcaactgcat aaccaatcaa aattaccgaa atttcatgca taatcacata aatcactttt
    32401 gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaac agtagcatga
    32461 aacgtcatta ccaattaagg cagtataaaa tgctggtttt ctcgtcagtt caaggcagga
    32521 taagggttaa cacgccttta tgacagtcag gaattggctg tttctctaac gacgcccctt
    32581 ttagccttaa ttagaaaatc cattttaatt tcagtcaatt aataaagaat tttgcgctaa
    32641 agcacatttc tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg
    32701 gaaatccgct ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga
    32761 gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc
    32821 gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg
    32881 gaagtggggt tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt
    32941 gagagggata actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag
    33001 gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc
    33061 gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc
    33121 atcgttgcgc aggagcgcgg ctggcgttta gcgaaaaact acgccgtcgg gatgctcttc
    33181 ctgaataaag atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agagctgcaa
    33241 cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt
    33301 gaaatcgccc tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc
    33361 tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt
    33421 ctcgaagccg acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa
    33481 ggtctgtgta tgccggcgga tctgccgcgc ttttatctag atcttgcgga cctgcgtctg
    33541 gaatcggcca tttgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg
    33601 ctggcgcaac cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac
    33661 cgccaatggg cgcgtgcgcg tacttataaa ttccagacac cgcttatccc tgacctgcac
    33721 gacgccgcac cgttcgtcaa cgaaatcggc tctgactcca gttcgatgga taacatgctg
    33781 gaactgctgc tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc
    33841 gcctggcaga acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc
    33901 atgcatatgg agccgtggga tggcccggcg ggcatcgtga tgtctgacgg tcgttttgct
    33961 gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct acgtcatcac caaagataag
    34021 ctcatcacct gcgcctctga agtcggtatc tgggattacc agcctgacga agtggtcgaa
    34081 aaaggccgcg tcgggccagg cgaactgatg gtaatcgaca ctcgcagtgg gcgtattctg
    34141 cactcggcag aaaccgatga cgatctgaaa agccgccacc cttataaaga gtggatggag
    34201 aaaaacgtcc gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc
    34261 gaactggacg acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag
    34321 ctggactccg taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc
    34381 gatgataccc cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc
    34441 cagcagtttg cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg
    34501 tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg
    34561 caccgtttaa gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg
    34621 atgaaagagg agcactaccg cgcagatacg ctggatatca cctttgacgt caccaaaacc
    34681 acgctcgaag cgacggtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc
    34741 accgtgctgc tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccggct
    34801 ccaatggcgg ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc
    34861 aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggt
    34921 ttcggcgcga cggctattta tccgtacctt gcctatgaaa cgctgggccg cctggtagac
    34981 acccacgcga ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac
    35041 aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc
    35101 tcgaaactgt ttgaagcggt cggtctgcac aatgatgtag tgggcctgtg cttccagggg
    35161 gcggtcagcc gcattggggg ggcgagcttt gaagacttcc agcaggatct gctgaatctc
    35221 tcgaaacgag cctggctggc gcgcaagccc atcagccagg gcggcctgct gaaatacgtc
    35281 cacggcggcg aataccacgc ctacaacccg gacgtggtgc gcacgctgca acaggcggta
    35341 cagagcggcg agtatagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca
    35401 accacgctgc gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgccgat
    35461 gttgaaccgg caagcgaact gtttaaacgc ttcgataccg cggcgatgtc tatcggcgcg
    35521 ttaagcccgg aagcccacga ggcgctggcg gaagcgatga acagcatcgg cggaaactcg
    35581 aactccggtg aaggtggtga agacccggcg cgctacggca ccaacaaagt gtcgcgcatc
    35641 aagcaggtgg cttccggtcg ctttggggtt actccggcgt atctggtcaa tgccgacgtc
    35701 attcagatta aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac
    35761 aaagtcactc cttacattgc caaactgcgc tattcggtgc cgggcgtgac gctgatctcc
    35821 ccgccgccgc accacgatat ctactctatc gaagacttag cgcagctcat tttcgacctc
    35881 aagcaggtta acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgttggc
    35941 accatcgcca ccggtgtggc gaaagcttat gccgatttaa tcaccatcgc aggctatgac
    36001 ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg caggctgtcc gtgggagctg
    36061 ggacttgttg aaacacaaca ggcgctggtt gccaacggct tgcgtcataa aatccgtttg
    36121 caggtcgatg gcggcctgaa aacgggtgtc gatatcatca aagcggcgat tctcggcgca
    36181 gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg gctgtaaata tctgcgtatt
    36241 tgccacctga acaactgcgc aacgggtgtg gcaactcagg atgacaaact gcgtaagaat
    36301 cactatcacg gcctgccgtt caaggtgacg aattactttg agtttatcgc ccgtgaaacc
    36361 cgcgagctga tggcacagct tggcgtaaca cgtctggtgg atctgattgg tcgcaccgac
    36421 ctgctgaaag agctggacgg tttcaccgcc aaacagcaga aactggcgct gtcgaagctg
    36481 ctggagaccg ccgaaccgca tccaggtaag gcactctact gcaccgaaac caacccgccg
    36541 tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgag
    36601 cgccagagca aaaccttctg gttcgatatc cgcaacaccg accgctctgt cggcgcgtcg
    36661 ctttcaggct atatcgccca gacgcacggc gatcaggggc tggcagccga tccgattaaa
    36721 gcgtacttca acggcaccgc aggccagagc ttcggcgtgt ggaacgcggg cggcgtggaa
    36781 ctgtacctga ccggtgatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc
    36841 gccattcgtc ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac
    36901 acctgcctgt atggagcgac gggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt
    36961 ttcggcgtgc gtaactccgg tgcaatcacc gtggtagaag gcattggcga caacggctgt
    37021 gaatatatga cgggcggtat cgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg
    37081 ggtatgaccg gcggcttcgc ttacgttctc gatgaaagcg gcgatttccg caaacgcgtt
    37141 aacccggaac tggtggaagt attaagcgtt gacgatttgg cgatccatga agagcatctg
    37201 cgtggtctta tcaccgagca tgtgcagcat accggttccc agcgtggtga agagattctg
    37261 gcgaactggt caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgtc
    37321 aaagcactgc tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa
    37381 ggggtagcaa caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg
    37441 ccaaagaaac cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc
    37501 gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag
    37561 tggaaatgcc cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt
    37621 atttttgaag cggcagaact gtcgcaccag accaacactc tgccggaagt atgcggacgt
    37681 gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc tgaacgatga gtttggcgcg
    37741 gtgaccatcg gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt
    37801 ccggatatgt ccggcgtgaa acagaccggt aaaaaagtgg cgattatcgg cgcaggtccg
    37861 gcaggtctgg cgtgtgcaga tgtcctgacg cgcaacggcg tacaagcggt cgtcttcgac
    37921 cgtcacccgg aaattggcgg cttgctgacc ttcggtattc cggctttcaa actggaaaaa
    37981 gaggtaatga cgcgccgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat
    38041 accgaagtgg gccgcgacgt acagctggac gatctgctga gtgattacga tgccgtgttc
    38101 cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc
    38161 gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggcgaa
    38221 actcgtgaag aaccgttcgt cagcatggaa ggcaaacgcg tggtagtact tggcggtggc
    38281 gataccgcaa tggactgcgt gcgcacgtct gtacgccagg gggcaaagca cgttacctgt
    38341 gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg
    38401 gaagaaggtg tagagttcaa attcaatgtc cagccactgg gaattgaagt gaacggtaac
    38461 ggcaaagtca gcggcgtaaa aatggtgcgt actgaaatgg gcgaaccgga tgccaaaggc
    38521 cgtcgccgcg cggagatcgt ggcaggttcc gaacatatcg ttccagcaga tgcggtgatc
    38581 atggcgtttg gtttccgtcc acacagcatg gaatggctgg caaaacacag cgtcgagctg
    38641 gattcgcaag gccgcatcat cgccccggaa ggcagcgaca acgctttcca gaccagcaac
    38701 ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg atctggtcgt taccgctatt
    38761 gccgaaggtc gtaaggcggc agacggcatt atgaactggc tggaagttta agctctgtcc
    38821 cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct cctttcgttc tggaaagcgc
    38881 ctcgcataat ctgaagaaaa ccaatgtaat acgcacatct ggattgttac cggcagatgc
    38941 ccggcgtata gctgattcca ttattcaaca ctgcgccata taaagggaaa gataatggaa
    39001 tcgctctcag agagaacctc aacaggctac cagcaaatcc acgacggtat tattcacctg
    39061 gtcgatagcg cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac
    39121 tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat
    39181 ggtgcgcagc taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc
    39241 tctgcaaaaa acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt
    39301 cgccagacag tgtctggcga attaacacca ttgccctggt ccacttacgt ccgtttactt
    39361 tccgttaaaa acgctgacac ccgcagcttt tatgaaaaag agacgctccg ctgtggctgg
    39421 tctgttcgcc agctagaacg gcaaattgcg acccaatttt atgagcgaac actgctgtca
    39481 catgacaaat cagccatgct gcaacaacac gctcctgccg agacgcatat tcttccgcaa
    39541 caggcgatac gcgatccctt tgtgctcgaa tttctggaat tgaaagatga atactcagaa
    39601 tccgattttg aggaggcgct gatcaaccac ctgatggatt tcatgctgga acttggggat
    39661 gattttgcct ttgttggtcg gcagcgaagg ttacgcattg atgacaactg gtttcgtgtc
    39721 gatctgctgt ttttccaccg ccgtttacgc tgcctgctaa tcgtcgatct aaaagtgggc
    39781 aaattcagct atagcgatgc cggacagatg aatatgtatc tcaactacgc caaagagcac
    39841 tggacgctac cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga gaaaggagtc
    39901 ggagaagcgc attatgcact ggctggtttg cctaacaccg ttctggcgag tgaatataag
    39961 atgcaactac ctgatgaaaa acgactcgcg gatgaactcg ttcgaacaca ggcggtgcta
    40021 gaagaaggct atagactccg ttaatttcag cggtttcgaa ttctgcagac actgtctgca
    40081 gaatctttaa aacgaactaa gttttaagcc attaaatcag ccttaacctt caccaccacc
    40141 tttttaatct caccaggttc gccgacaacg catcctggtt tatgcggttc tcccggcata
    40201 aacacggcga acattcccgg ttttaagttg atggtttgct cgttttcaat ggcgctgcaa
    40261 agctggtaat catcttcatg gtggaactct tcacactggc gcgcagtgcc tgccgtgcca
    40321 aacagaatcc gttcctcacc ttttaataac agctggatat caatgtattg ctcgtgcaat
    40381 tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaaaatattg
    40441 tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc taatgccagc
    40501 gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc
    40561 atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcatggc
    40621 ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt
    40681 ccaccagcgc cagataccct tctgccagac caacgctgcc accgaccacc acgcactgac
    40741 aatcagttgt ggctttaata tcagcgatca gccttgccag cacatgtgcg gagcggtgaa
    40801 tcagctgctg cgcctgctcg tcgccctgcc cggcgcgcat gaaaatagtt ttcgcatccg
    40861 cgccagccaa ctccccctgc gctgccgctg caataccgcg accagaggca atcgcttcca
    40921 cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc gtatgcccga
    40981 tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc
    41041 cgacgccagt ggaaacggtg ataaacacca tatcggttat atcgccttcc agcgcctgat
    41101 actctgccca tgctgcggcc tgggcgtcgt taatggcaat ggtcggcaaa tcggtaagtt
    41161 gccccagcgt tttgactaac ggaaagtgta gcaatccacc aagattatgc ggattcagcg
    41221 ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag
    41281 cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg
    41341 ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg
    41401 caagtttagt accgccgata tcaatcgcca gtgtggtcat agcaccgcct ttttcatcgc
    41461 tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc
    41521 ccacgcgccg tggcgcatcg catcagccgc ctgagcaggc gtgttgtaac gcccttcggc
    41581 aatcacccga catccggcgt cgctcaacct tttcaccagc gtcagatccg gctcttctgg
    41641 cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg
    41701 gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatgatg
    41761 aatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc
    41821 gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg ccgtgatgcg
    41881 taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc tcaccaccgc
    41941 acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc ccgcctgctc
    42001 tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt ccggaaccgg
    42061 ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa
    42121 cgacatacat cttcccttag cgaaaggccc gatacagaga ccgggcaaca ggattaactt
    42181 ttggttttga ctaaatcgtt tttggcgctg ccaaacggca cggcaccgct aaatggtttg
    42241 ccgtcgatag cgtcatgagt acgcaacgct tccgggcgca gccaacgctg aacacgagaa
    42301 ggcatatcca gaccaatcag caggatcacc acaaatgtca gactgaacga gagcgatgcc
    42361 agtgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg
    42421 ccacccaatg cgccaacgtt ataggtaaag cccaggcccg ctgcacgctg gtcggtatcg
    42481 aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg
    42541 aagaacagta acagaccgag cacccagacg tttgcaccgc caatagcaaa taccggaata
    42601 atcagcagct gcgaggccaa cagactacaa acgtacgctt tgcgggttcc cagccagtca
    42661 ccgaggaagc caccgacgca gcaccccacc gccgcgccaa agccactaaa gaacagcaca
    42721 ttggcgacag tatgagggtt ataagccaga tcggttttca gatacgttgg cagcagcgcc
    42781 tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgccagtt
    42841 ggccagcgtt tgcccgtact ctgcaccata aagctgataa agattgcggt gcataacagc
    42901 ccaagaacag cgacgatcgc ggcattttgc aggttaccgg cgaagcagaa ccacagcgca
    42961 gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata
    43021 tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc ttctgcttcc
    43081 gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc
    43141 gcacgccagc cccagaccgg aaccaccagg ctgtagacct gagcggcaac gacggcccct
    43201 acagagaagc ctgaaatcag aaagccactg gctttgttac gcagatgttt tggccagctt
    43261 tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga
    43321 cgagcgataa acatggtgat gtagcctggc gcaaagccgc atgccagcgt cccggccgag
    43381 aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg
    43441 agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc
    43501 accgtcgtca gcccgaattc accttgtact tcggtgagta ccagggcgat taaaacgaaa
    43561 tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcccgcca ttgtgcacga
    43621 ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg
    43681 tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt ttatttcccc
    43741 tcacccggta ggggcgagcg aggggaaaca actcacccgc gctcttgcat caactgctgg
    43801 gccagcgcct tcagttctgg cagatatttt tcatctaccg gaccaaacgg tttgcggcac
    43861 agcggcacag aaacgacatc catataatgc agaacagttt tcaggccgcg gaatacaccc
    43921 gttttgatca gtaaatcaat gactttgttg cattcagttt gcagtttctg cgcggtctgg
    43981 atatcgcctt ctttcagcgc cttaacgatc ccctgatagc gccagcccat gatgttgtag
    44041 gtactaccga taccaccatc agcgcccgcc agcaggccag aggcgaagat ttcgtcgtaa
    44101 ccgttataga gcaccagatc aggatgttca cgacggatct gctccatctg atagagatcg
    44161 ccagaggtct gtttcagcgc acctacgcca ggcaatgtaa caagtgtgtt gatctgatcc
    44221 agggtcagtt ttaccccact cagggctgga atgttgtaca ccaccatcgg caaaccatcc
    44281 gccgaatcaa taattgcccg atagtgatcg cagtgttctt caaagctgaa aggatagtag
    44341 aacggcgtga cggcggagac ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt
    44401 tggctttcgg cggtgctgac gcaaccgacg tgggcgatga gtttaatctt acctttcgcc
    44461 tcttcggcga cgatttccag tacctgttca cgctcggaaa ggctttgtac aaaggcttcg
    44521 ccggtcgaac cacccacgta taaaccgtca atgccctgct gaatattgaa ctgaaccagg
    44581 cggcgcagac tcgctttatc cagtgcttgt tgttgatcaa aaggagtcag gagtgcagcc
    44641 attacgccac gtaaattcgt tgccataaat acctctgaag tgatgcttgt ctgataaacg
    44701 atataccttt atacctgtta taccagatca attaagcaac accccataca gaaagcttat
    44761 aatgcgatct gcttcactaa agtggcatta tttctttttg ttggtggtct gaccgaaggc
    44821 gtgccaggta gcagagacgc tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg
    44881 atcgtggcgg cggatcgcgt caacgatcgc aatatgctgt tgatagctaa cgttgttatg
    44941 ctcgtgcaga gcctgatcag ccacggttgg acgtgcggcg atcagccagt cgagcagggc
    45001 aacgtggatc gccatgaaga ttgggttacc ggggatctcc gccagcacgc ggtggaaatc
    45061 aacgtctgag cgaatgaatg ccgcgttgtt atccagcgac tgactgttga tttccagtgc
    45121 ttttgccagc aaatcgattt gctcatcggt ggcgtgttca gccgcatagc gcaccaggct
    45181 ggattcaaag aacagacgta attgttcgaa atgggcaatc ccaccaggat gagaaaggaa
    45241 atctttcgcc atgccggaaa gctcgccgat gatggtgtcc gcagaaggac gtgagacgcg
    45301 ggcacgttcg ccgttgttta tttgcaccag acctttgcgt tttaacgctg ccagcgcttc
    45361 acgcaccgaa ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag acggtaattg
    45421 ttcaccttcg ccaaattcac gacggcggat catctgttcc agctcttctt ccaccatttc
    45481 ggagagtttt ttacgcgcca gcgggcggct acgcaagttg cgaccaattg caggtgaaga
    45541 atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta
    45601 acatcatcag tgacatccta tcacaggatt gaaagtaggg gaaaatggca gggttttctc
    45661 tttgtgcctc atcattacca taattaacgg aataattaac tattgcgaaa aaataatgta
    45721 acgcagataa aaacatcc
//
LOCUS       DBFIFS010000011        44359 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.011, whole genome shotgun sequence.
ACCESSION   DBFIFS010000011 DBFIFS010000000
VERSION     DBFIFS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.011"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(260..802)
                     /gene="ycfP"
                     /locus_tag="N7A07_000613"
     CDS             complement(260..802)
                     /gene="ycfP"
                     /locus_tag="N7A07_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU2475341.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(825..1850)
                     /gene="nagZ"
                     /locus_tag="N7A07_000614"
     CDS             complement(825..1850)
                     /gene="nagZ"
                     /locus_tag="N7A07_000614"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU2475342.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1861..2685)
                     /gene="thiK"
                     /locus_tag="N7A07_000615"
     CDS             complement(1861..2685)
                     /gene="thiK"
                     /locus_tag="N7A07_000615"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU2475343.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2666..3307)
                     /gene="lpoB"
                     /locus_tag="N7A07_000616"
     CDS             complement(2666..3307)
                     /gene="lpoB"
                     /locus_tag="N7A07_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU2475344.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3321..3698)
                     /locus_tag="N7A07_000617"
     CDS             complement(3321..3698)
                     /locus_tag="N7A07_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU2475345.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3701..4060)
                     /gene="hinT"
                     /locus_tag="N7A07_000618"
     CDS             complement(3701..4060)
                     /gene="hinT"
                     /locus_tag="N7A07_000618"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU2475346.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4191..4379
                     /locus_tag="N7A07_000619"
     CDS             4191..4379
                     /locus_tag="N7A07_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475347.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAF
                     ASKIIRTLNITAHHPPPA"
     gene            4412..6601
                     /gene="fhuE"
                     /locus_tag="N7A07_000620"
     CDS             4412..6601
                     /gene="fhuE"
                     /locus_tag="N7A07_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU2475348.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6661..8094)
                     /gene="ptsG"
                     /locus_tag="N7A07_000621"
     CDS             complement(6661..8094)
                     /gene="ptsG"
                     /locus_tag="N7A07_000621"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU2475349.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8389..9186)
                     /locus_tag="N7A07_000622"
     CDS             complement(8389..9186)
                     /locus_tag="N7A07_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2475350.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9197..10201)
                     /gene="holB"
                     /locus_tag="N7A07_000623"
     CDS             complement(9197..10201)
                     /gene="holB"
                     /locus_tag="N7A07_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU2475351.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10198..10839)
                     /gene="tmk"
                     /locus_tag="N7A07_000624"
     CDS             complement(10198..10839)
                     /gene="tmk"
                     /locus_tag="N7A07_000624"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU2475352.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(10829..11851)
                     /gene="yceG"
                     /locus_tag="N7A07_000625"
     CDS             complement(10829..11851)
                     /gene="yceG"
                     /locus_tag="N7A07_000625"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU2475353.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11854..12663)
                     /gene="pabC"
                     /locus_tag="N7A07_000626"
     CDS             complement(11854..12663)
                     /gene="pabC"
                     /locus_tag="N7A07_000626"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU2475354.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12783..14024)
                     /gene="fabF"
                     /locus_tag="N7A07_000627"
     CDS             complement(12783..14024)
                     /gene="fabF"
                     /locus_tag="N7A07_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU2475355.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14112..14348)
                     /gene="acpP"
                     /locus_tag="N7A07_000628"
     CDS             complement(14112..14348)
                     /gene="acpP"
                     /locus_tag="N7A07_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2475356.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14559..15293)
                     /gene="fabG"
                     /locus_tag="N7A07_000629"
     CDS             complement(14559..15293)
                     /gene="fabG"
                     /locus_tag="N7A07_000629"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU2475357.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15306..16235)
                     /gene="fabD"
                     /locus_tag="N7A07_000630"
     CDS             complement(15306..16235)
                     /gene="fabD"
                     /locus_tag="N7A07_000630"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU2475358.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16251..17204)
                     /gene="fabH"
                     /locus_tag="N7A07_000631"
     CDS             complement(16251..17204)
                     /gene="fabH"
                     /locus_tag="N7A07_000631"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU2475359.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17272..18342)
                     /gene="plsX"
                     /locus_tag="N7A07_000632"
     CDS             complement(17272..18342)
                     /gene="plsX"
                     /locus_tag="N7A07_000632"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU2475360.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18423..18596)
                     /gene="rpmF"
                     /locus_tag="N7A07_000633"
     CDS             complement(18423..18596)
                     /gene="rpmF"
                     /locus_tag="N7A07_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU2475361.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18648..19169)
                     /gene="yceD"
                     /locus_tag="N7A07_000634"
     CDS             complement(18648..19169)
                     /gene="yceD"
                     /locus_tag="N7A07_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU2475362.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19368..19952
                     /gene="yceF"
                     /locus_tag="N7A07_000635"
     CDS             19368..19952
                     /gene="yceF"
                     /locus_tag="N7A07_000635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU2475363.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20053..21012)
                     /gene="rluC"
                     /locus_tag="N7A07_000636"
     CDS             complement(20053..21012)
                     /gene="rluC"
                     /locus_tag="N7A07_000636"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU2475364.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21585..24770
                     /gene="rne"
                     /locus_tag="N7A07_000637"
     CDS             21585..24770
                     /gene="rne"
                     /locus_tag="N7A07_000637"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU2475365.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24965..25918)
                     /gene="flgL"
                     /locus_tag="N7A07_000638"
     CDS             complement(24965..25918)
                     /gene="flgL"
                     /locus_tag="N7A07_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU2475366.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25930..27573)
                     /gene="flgK"
                     /locus_tag="N7A07_000639"
     CDS             complement(25930..27573)
                     /gene="flgK"
                     /locus_tag="N7A07_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU2475367.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27639..28580)
                     /gene="flgJ"
                     /locus_tag="N7A07_000640"
     CDS             complement(27639..28580)
                     /gene="flgJ"
                     /locus_tag="N7A07_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU2475368.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28580..29677)
                     /gene="flgI"
                     /locus_tag="N7A07_000641"
     CDS             complement(28580..29677)
                     /gene="flgI"
                     /locus_tag="N7A07_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU2475369.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29689..30387)
                     /gene="flgH"
                     /locus_tag="N7A07_000642"
     CDS             complement(29689..30387)
                     /gene="flgH"
                     /locus_tag="N7A07_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU2475370.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30440..31222)
                     /gene="flgG"
                     /locus_tag="N7A07_000643"
     CDS             complement(30440..31222)
                     /gene="flgG"
                     /locus_tag="N7A07_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU2475371.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31360..32115)
                     /gene="flgF"
                     /locus_tag="N7A07_000644"
     CDS             complement(31360..32115)
                     /gene="flgF"
                     /locus_tag="N7A07_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU2475372.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32135..33340)
                     /gene="flgE"
                     /locus_tag="N7A07_000645"
     CDS             complement(32135..33340)
                     /gene="flgE"
                     /locus_tag="N7A07_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU2475373.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33365..34060)
                     /gene="flgD"
                     /locus_tag="N7A07_000646"
     CDS             complement(33365..34060)
                     /gene="flgD"
                     /locus_tag="N7A07_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU2475374.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34072..34476)
                     /gene="flgC"
                     /locus_tag="N7A07_000647"
     CDS             complement(34072..34476)
                     /gene="flgC"
                     /locus_tag="N7A07_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU2475375.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34480..34896)
                     /gene="flgB"
                     /locus_tag="N7A07_000648"
     CDS             complement(34480..34896)
                     /gene="flgB"
                     /locus_tag="N7A07_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU2475376.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35051..35710
                     /gene="flgA"
                     /locus_tag="N7A07_000649"
     CDS             35051..35710
                     /gene="flgA"
                     /locus_tag="N7A07_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU2475377.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35786..36079
                     /gene="flgM"
                     /locus_tag="N7A07_000650"
     CDS             35786..36079
                     /gene="flgM"
                     /locus_tag="N7A07_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU2475378.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36084..36500
                     /gene="flgN"
                     /locus_tag="N7A07_000651"
     CDS             36084..36500
                     /gene="flgN"
                     /locus_tag="N7A07_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU2475379.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36540..38075)
                     /gene="murJ"
                     /locus_tag="N7A07_000652"
     CDS             complement(36540..38075)
                     /gene="murJ"
                     /locus_tag="N7A07_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU2475380.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38185..39108)
                     /locus_tag="N7A07_000653"
     CDS             complement(38185..39108)
                     /locus_tag="N7A07_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2475381.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39110..39757)
                     /locus_tag="N7A07_000654"
     CDS             complement(39110..39757)
                     /locus_tag="N7A07_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU2475382.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39768..40352)
                     /gene="rimJ"
                     /locus_tag="N7A07_000655"
     CDS             complement(39768..40352)
                     /gene="rimJ"
                     /locus_tag="N7A07_000655"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU2475383.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40588..41796
                     /gene="mdtH"
                     /locus_tag="N7A07_000656"
     CDS             40588..41796
                     /gene="mdtH"
                     /locus_tag="N7A07_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU2475384.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41860..42507
                     /gene="grxB"
                     /locus_tag="N7A07_000657"
     CDS             41860..42507
                     /gene="grxB"
                     /locus_tag="N7A07_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU2475385.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42641..43201
                     /locus_tag="N7A07_000658"
     CDS             42641..43201
                     /locus_tag="N7A07_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2475386.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43307..44353
                     /gene="pyrC"
                     /locus_tag="N7A07_000659"
     CDS             43307..44353
                     /gene="pyrC"
                     /locus_tag="N7A07_000659"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU2475387.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        10668 a        12026 c        11081 g        10584 t
ORIGIN      
        1 tgttaatcaa gttttgttgc tgaaaagata ggcgacttta ccgggtgaga tcaaaaaaca
       61 gagtttattg tttattattt gagcgaattt gtgagagtga tatagcgtga ggggtgtatg
      121 tagggcgttc ggtaggtctg ataagaggcg catcaggcat tgatgctcga ctgtcggatg
      181 cggcgtgaac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
      241 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgcaaatgcg
      301 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
      361 gcaactcttc agatgtccgc tggctgttaa gcgcttcatc attacgcgac aaaatcacca
      421 ggcaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
      481 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
      541 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccagac
      601 caacgccgca aatcagggga cgctcgtcga cgttcagttg caacattttg tccacttctt
      661 tgagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
      721 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
      781 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc
      841 tcttcctgcc agcgttcatg taactgattc agacgggcgc tgatcgcttt ccagcgagcc
      901 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
      961 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
     1021 aggatcatat cgcaacccgc atccagtgat acctgcccgc gttcggcata actgcccata
     1081 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa acccagttcc
     1141 tgacgcaaaa cggttttcag ccagtaggaa gaaccgctcg ccggacgcgg atcaacatca
     1201 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taacgtgctg
     1261 aaaaccgaca tatctttggc acgaatttcc gcttgcgggc gtgggtcgca cggcgtctct
     1321 ttgtgtgaat ctgcagttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
     1381 ccggcttcat gcatcccatc aataaaccga ctggcgattg ccagggcttt ttctgggtcg
     1441 gcatgataag aacgctcgcc aatcgccgcg ctgatatgtc ctacatccag cactggcgca
     1501 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
     1561 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
     1621 cgggtaaaac cttcgcgaaa acgctggacg cgtccacctt cctgatctac cgccaccacc
     1681 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgcaactg ggcaggatca
     1741 tgatagtttc gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
     1801 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
     1861 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
     1921 gccggtttgt cgccagcggt actcaaacca ccctgctttg agcatcagca gccagggaaa
     1981 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
     2041 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
     2101 gatatcacca tctcctgcat actcccagtc gatgagtttt aaccctgacg cgctatgcac
     2161 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggcc gtggttcccg
     2221 cgctttgcgc agacgtttta acattcgcag ccaacccact gtccgccgcg ccggatcgct
     2281 ttgctgccag tacagttcca gtaacggcaa cagcgttatt cgccagccaa aacgtggttg
     2341 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
     2401 ctcgccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
     2461 aatgcatgcg ggtagttgtg ataaagcccg atactggtgc aagaacgcgg actgcggcgc
     2521 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
     2581 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
     2641 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
     2701 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
     2761 cagaggcgct ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
     2821 tactacgggt gcctaaacta tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
     2881 gctgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
     2941 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
     3001 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
     3061 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgcagtttga tcttcgtgct
     3121 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
     3181 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
     3241 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
     3301 ttgtcatcaa gattcacccc ttacaaatat agataaagtc tgactttgtg cgcccccaga
     3361 aaattggcgc tgccgtacag cgttaccgcc gaatgtgcgg gaatggtaac gctgcgtggc
     3421 ctttccagag gatgcatctc cagccctctg gcgtcatacc agtaaaaacg ataatgaacg
     3481 gtaacgggtt cttgcctttc gttataaagc gttgaggatg ctgaaggttg aatatcagac
     3541 gtcgaaagga cgggcttttc tgcactgatg cccgcagcca ataaagatga ctccatcacc
     3601 agcgattgct catcattcac cggaatttcc ggatgtgaac gacagcccac cagcagtaac
     3661 aacgccagag acaccagccc aaagcatcct tttctcatcg ttacagacct ttatgcgcca
     3721 gcattggtcc cagcggacgg ccacccaaca agtgcatatg gatgtggtaa acctcttgtc
     3781 cgccatggcg gttggtgttc atgatcagac gatagccatc ttcggcaata ccttcttgct
     3841 cagcaatttt tgccgctacg gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaga
     3901 cgtcgttcac agtcggaatg aggatattcg gaatgatcag aatatgcgtt ggcgcttgcg
     3961 gcgaaatatc gcgaaacgcc gttaccagat catcctggta gacgatatcg gaggggatct
     4021 cacgacgaat aattttgctg aatatagttt cttctgccac gacattttcc tttttcaaat
     4081 agccatgcgt tgcgccatgc tacgccgcgc cacactgcga gtatagagta tggtcgagtt
     4141 aaacctgctc tttcaacctt aactcacgat ttcttaagca aaaaatcgtt ttgctgccgt
     4201 atatatctcc attattccca tttctgcctg cgaatgcgct gaaaagacat tctctgtttg
     4261 gtttacacga caaatatgaa tgcgtatatt tctcatttgc atttgcattt gcatttgcat
     4321 ttgcaagcaa aattattcgc acactaaata taaccgctca tcaccctcct ccggcatgag
     4381 cctgacgaca acataaacca agagatttca aatgctttca acacaattta acagggataa
     4441 tcaatatcaa gccatcacca aaccgtcact acttgccggt tgcatagcac tggcactatt
     4501 accttctgcc gcttttgctg caccagccac tgaagaaacg gtgattgttg agggttcagc
     4561 cacagctcca gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa
     4621 aatgcagatg actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat
     4681 ggaagatcag cagttacaaa cgctgggcga agtgatggaa aacacgctgg ggatcagcaa
     4741 aagtcaggcg gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta
     4801 tatggttgat ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc
     4861 tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg
     4921 gacgggtaat ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt
     4981 taaaggcgat gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt
     5041 acaaagccca ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa
     5101 taacgactca tggctggacc gctacaatag tgaaaagacc ttcttctccg gcattgtcga
     5161 tgctgattta ggcgatctta cgacgctttc agccggttac gaatatcagc gcattgatgt
     5221 taatagccca acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta
     5281 cgatcgcgca cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt
     5341 ctttatgacc ctgaagcagc ggtttgctga tacctggcaa gcgacactga atgccaccca
     5401 ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga
     5461 tggtatgctg gttgggccat acagtaatta tggacctggc tttgattatg tcggcggcac
     5521 cggttggaac agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtagtta
     5581 tgaattgttt ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa
     5641 tcgttacttc agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt
     5701 taatggcaat ttcccacaaa ccgactggtc accacagagc ctggcgcagg acgataccac
     5761 acatatgaaa tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat
     5821 cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa
     5881 ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc
     5941 cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc
     6001 tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct
     6061 gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac
     6121 acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa
     6181 aggggtggaa tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc
     6241 aacgcgctat attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac
     6301 cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg
     6361 tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg
     6421 cgccgagcaa ggtagctacg cgttggtgga tctcttcacc cgctaccagg tgacgaaaaa
     6481 cttctcgtta caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga
     6541 aggttctatc gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg
     6601 aataacacct gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacgga
     6661 ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa
     6721 aatcgcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc
     6781 agcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc
     6841 gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct
     6901 ggtacctgtc gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag
     6961 tgctttaatc agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat
     7021 cgggaacagc cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg
     7081 cgaaaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc
     7141 cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg
     7201 ctcggtgata ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt
     7261 cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta
     7321 cattttgaac aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg
     7381 cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg
     7441 gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc
     7501 gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat
     7561 tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag
     7621 gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt
     7681 aatacggtag aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccctccgag
     7741 tacgccagta tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag
     7801 tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg cagccagcgc
     7861 ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat
     7921 gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca
     7981 gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg
     8041 cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga
     8101 gagtgctcct gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc
     8161 tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt
     8221 gccaatttgt ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt
     8281 tgacaaaaca tatgtcacaa tatttcaaac gtggttattc accgcccaat tttcggcggt
     8341 gaactttact cgttttagtc attaattacg agctttaaaa aaactcattc aacggatgga
     8401 ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc
     8461 cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat
     8521 cgcaggttga ttctcttttc ctcgatgcgg caccggcgca aggtaaggtg agtcagtttc
     8581 caccagtaac cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg
     8641 gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt tgcccgccgt
     8701 ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg
     8761 cagaattgcc agcgtatcgg cgcgggcgtc acgggtatgg acgataaccg gcttgttcag
     8821 ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg
     8881 tgtgtaataa taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag
     8941 acggcgtaaa tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga
     9001 aaatacgacg ttgtcacgtt cgcctaccag atcccgcatg tgtaaataac ccggtaatgt
     9061 tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc
     9121 cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa
     9181 aaacatgatg tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat
     9241 gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtgacagaca
     9301 ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa
     9361 gatggtttgc cagttcggcg accaggcccg gcacatcaac attggtcacc tgcgcagcgc
     9421 catgatggcg ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag
     9481 cttgttcatg attaagggcc gccagcagcg aataccagtc gcccgatggc acgctatatg
     9541 ccaacgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg
     9601 ccgcgccagg cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg
     9661 tcacttcgcg tgaaagccag gtcacggcgt actgttccgg cggcggcgca aggtaatgta
     9721 accgacaacg actacgtaac gttgccagta aacgttcagg ctcgcgggta gccaggaaaa
     9781 accaggtttc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg
     9841 tcagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaagcgt gcgtgctcat
     9901 tcagcttttc ggtgacctca cgtaccgcat caatgcccag cgtatttttc cctttttcgg
     9961 gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt
    10021 gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga
    10081 ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc
    10141 cggcctgata gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca
    10201 tgcgtccaac tccttcaccc agcgggtcac ggtagtgcgg attgcatcca tcacggcctc
    10261 cagcggctgg gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata
    10321 gcgggcgcgg gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc
    10381 gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc
    10441 ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc
    10501 acgtcctccg ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa taacccaggt
    10561 gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa
    10621 catcagaact tcggctttat cggtaatgat ttcatcgcct accgatttga tatccagcac
    10681 caggcttctt aacttttcgg caagttgcgt accaccaggt tcccgagtga aaaccatgtc
    10741 gcggataccc agttgctcga gcgtctcaac caccacatta cgcgcggtag ttttgcctgc
    10801 gccttccagc ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca
    10861 gataatcctg cacagacttg ttatgactgg caagattggt attaaacgtg tgaccacctt
    10921 taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg
    10981 aatccgcccc cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat
    11041 acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc
    11101 cgtaaatcac ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata
    11161 ccgaggcaac ctgatcgcgt tcactggcaa tggcggtttc tttttcgata attgatgcca
    11221 tcgtcaccag ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc
    11281 tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat
    11341 tggcggtata catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca
    11401 gttcaagtgc ctgcgctacg gtgatgtact tatcatcgct cagcgtgtgc ttgatatacg
    11461 gggcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagag
    11521 ggaactgtgc ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg
    11581 gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcaacc
    11641 attgaaaaac ccgtgggcga ttgatgatct tatcggcata aagctgttca ccaagcgcca
    11701 gacgtccggt ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt
    11761 cggcaagata gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca
    11821 ataacaagat tatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc
    11881 taaatattca tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat
    11941 cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga
    12001 ggcttgcact tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg
    12061 cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacgt tgcccttccg
    12121 ccagaacaaa ttagctgcac agcattccgt aaccccccct tcgctgtcaa ggaccagcgc
    12181 ctcatcagcg tttgtctgct caagatgaga gcggatcaat acttgctcaa gccgattaag
    12241 atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt
    12301 gatcccttcg ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg agagaatccg
    12361 cgttgccggt ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac
    12421 gactttcagt acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg
    12481 aggccagaag tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg
    12541 cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc
    12601 aaactgcgtt gcccgatcgc ttactgacag cgattcctgc ttataaccgt taattaagaa
    12661 cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc
    12721 ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca
    12781 acttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac
    12841 agcgtgtatt ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa
    12901 ccttcatccg ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg
    12961 atggagtaga tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc
    13021 gtggagctta ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct
    13081 tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc
    13141 gcttcaatgc ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt
    13201 tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg
    13261 taaattttcg caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca
    13321 ccatcgccca gtacgaaacc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg
    13381 ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacacc cagcggcgta
    13441 ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc
    13501 gcagcatggc caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg
    13561 ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga
    13621 acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg
    13681 atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct
    13741 tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga
    13801 atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc
    13861 tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg
    13921 atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga
    13981 gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga
    14041 gggaaaaaat gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg
    14101 gagatgttca cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg
    14161 attttctcag cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc
    14221 agctcaacgg tgtcaagaga atccgcgcct aggtcttcaa cgaaagaagc attgttggta
    14281 acttcttcct gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata
    14341 gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat
    14401 aaaatgttga aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt
    14461 tgaggcctta ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc
    14521 agactgcgcg caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg
    14581 ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc
    14641 gcgttggcga tttcctgtgc gccgccgagg cgacccgcag gaacctgcac caggataccc
    14701 gcacgctggt catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca
    14761 acgtttacag taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc
    14821 aagcccgctt tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca
    14881 gaaccgatag tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt
    14941 gacagacgga aaacagatga aaggttggtt tcgataatat cgttccactc ttcatctttc
    15001 attcgcatta acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca
    15061 ccaaattctg cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc
    15121 aacatcagac ctttgccgtt agcacctaaa taatcactga tcgcctgagc gccattttca
    15181 ctggtcgcag tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg
    15241 ccaattccgc ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgatgttc
    15301 ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc
    15361 agggtgtcga caatgcgttt cgtcaggcca gtaagcactt tgcccgggcc gacttcatag
    15421 agatgttcta cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta
    15481 tacaactgac gtaccagtgc gtcacgaatg gcatcaccat tggtttcgca tttcacatca
    15541 acgttattca caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc
    15601 agtttgtcgg ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc
    15661 agagcgcgtt ttgcgcccgc agctttacag gcagcgccag cacgctcaac cgcttcttta
    15721 tgaccggcaa taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct
    15781 tctgcagctt cttcacacgc ttttccaata gacgcatcat ccagaccgat gattgccgcc
    15841 atagcgcccg ttccttccgg tacggcttct tgcatgaact tgccgcgcat ctcaaccaga
    15901 cgcaccgcat cagcgaaatc aatcacacca gcgcaaacca gcgcggagta ttcccccagg
    15961 ctgtgaccgg ccatcattgc cggtgcttta ccgccatgct gctgccatac gcggtacagc
    16021 gcaacagagg cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct
    16081 ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac
    16141 gtttcttcga caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa
    16201 ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga
    16261 accagcgcgg agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc
    16321 ggcttgatgc gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag
    16381 gtattaccgt ggcgatccag cgtcaccacg acattgtcca tagacatacc gagttttttc
    16441 gccgttgcac tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa
    16501 cggtcaagat tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca
    16561 accttgaaga cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg
    16621 tcggcgtttg gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa
    16681 atgattcccg gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata
    16741 ataatagtcc cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc
    16801 agagcatact tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg
    16861 aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc caacatgctt
    16921 tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc
    16981 aggccaatct ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag
    17041 cccatggttg aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga
    17101 gtgacaatcc actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact
    17161 tgttcgggca gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt
    17221 ttcggttata taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag
    17281 agttccgctt ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg
    17341 agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac
    17401 cgcaaaagct cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa
    17461 cagacaggcg ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt
    17521 ttgtagccaa cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga
    17581 tttcagcaga gaaaggaaca tcctgacaac accttccatc gtctttaatg tgacatttcc
    17641 tgtaaagccg tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag
    17701 atagccgata taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact
    17761 atcgagaccc tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat
    17821 ttccaccacc tcttcagcca ggactgagcc cataatggca aattgcacca acattgtgct
    17881 gtcacaatcg acgttggccc ctaagtcaag gaccaccgtt ttgccctttt gctgatgtgg
    17941 taataccgtc accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc
    18001 cagccccatc agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt
    18061 caccagctcc agggccacgc gcattgaact cccacgactg gcgcggatag cttgcgaagg
    18121 ccgggcatca ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc
    18181 aaagtcagct ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt
    18241 gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa
    18301 atcccctccc atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg
    18361 tttggtcttt ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt
    18421 gattacttag cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc
    18481 aggtgttttt caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca
    18541 tgggaacgac gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac
    18601 cttactcctc aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt
    18661 aagctggcta atacggcaaa tgggtttggc ttttgcgctt cttcaggcag ttcaccaaag
    18721 accatgtccg cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag
    18781 gcgaggatga tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc
    18841 tcaatcggtt catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta
    18901 aaacaatacg ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc
    18961 gttaccgtca ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac
    19021 gacatggagc attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc
    19081 tgatcaggag tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg
    19141 agagtcaggg gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata
    19201 gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg
    19261 ttactgcttt attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat
    19321 agtttaaaat ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta
    19381 ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg
    19441 aatgtgcagc acccgaggtc gacgaaactc cacacagcga cgaatcaccg cgacagttgg
    19501 tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa
    19561 ttattggttc agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg
    19621 aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcact ttctatactg
    19681 gactggcgtt gtttaattcg gcgaatgggc atctgcaaac agaagtggag ccttttgacg
    19741 tccatttccg tcacctgagc gaggcggaga ttgataatta cgtgcgtaaa gagcatcccc
    19801 tgcactgcgc gggtagcttt aagagtgaag gatttggcat tactctgttt gagcgtttag
    19861 aggggcgtga tcctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac
    19921 gtcgggaagg gaaaaacccg ctgatgggat agttttttgc gccggatgcg gttacgtcgc
    19981 atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg ccttatccca ttatcatgct
    20041 tatatgctcg tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccatcatc
    20101 catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc
    20161 ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc
    20221 acggtcaccg tagcgatcgt caaaggcaat cggatgacct gcatactgtg tatgcacacg
    20281 gatctgatga gtgcgccctg ttaccggact acaacgcacc agggtggcaa atgcatagcg
    20341 ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactcacac gcacgatacg
    20401 ttcgccgctt tgcagaatat ttttcaataa cggcgcctga acgctcttca catgcgactg
    20461 ccactgaccg cgcaccagcg ccaggtaatc tttttgcatc cctttttcac gtaattgctc
    20521 atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg aggtgtcccg
    20581 gtcaagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc
    20641 aataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag
    20701 caccaggatg tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag
    20761 atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggaa tacgcacctc
    20821 atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcgcc
    20881 tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa
    20941 gttgtcgata cgttgccccg cttcgtcggc ggtgatagca acaattttta cggatggagt
    21001 ctctgttttc atggtgggcg attctaatta gccaacagga ttcgcgccac tcatttttct
    21061 atgcttatat ttagttttgc accttattac ttcactacgt gatcacttta ttgatggtta
    21121 ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa
    21181 agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg tgtccatcct
    21241 tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat ccacgcagca
    21301 atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc agtccttacc
    21361 ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata attgcgctgt
    21421 ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga cgataaccgt
    21481 gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag gcgttacttt
    21541 gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa agaatgttaa
    21601 tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg
    21661 acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa
    21721 tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct gaacgtcacg
    21781 gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac agtgctcatg
    21841 gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt cagatcgata
    21901 aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg gcgggtagct
    21961 atctggttct gatgccgaac aacccgcgcg cgggtggcat ttctcgccgt atcgaaggcg
    22021 acgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa ggcatggggc
    22081 ttatcgtgcg caccgctggc gtcggcaaat ctgctgaggc gctgcaatgg gatttaagct
    22141 tccgtctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg gccccgttcc
    22201 tgattcatca ggagagcaac gtaatcgttc gcgcattccg cgattactta cgtcaggaca
    22261 tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag catatcgctg
    22321 cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag atcccgctgt
    22381 tcagccacta ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc
    22441 catccggtgg ttcgattgtt atcgacagca ccgaagcgtt aacggccatc gacatcaact
    22501 ccgcacgcgc gacccgcggc ggcgatatcg aagaaaccgc gtttaacact aacctcgaag
    22561 ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg attgttatcg
    22621 acttcatcga catgacgcca gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag
    22681 cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg
    22741 aaatgtcccg ccagcgcctg agcccatcac tgggtgaatc cagtcatcac gtttgtccgc
    22801 gttgttctgg tactggcacc gtgcgtgaca acgaatcgct gtcgctctct attctgcgtc
    22861 tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt gttcctgtgc
    22921 caatcgcttc ttacctgctg aatgaaaaac gttctgcggt aaatgccatt gaaactcgtc
    22981 aggacggtgt gcgctgtgta attgtgccaa acgatcagat ggaaaccccg cactaccacg
    23041 tgctgcgcgt gcgtaaaggg gaagaaaccc caaccttaag ctacatgctg ccgaagctgc
    23101 atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag cgtccggaac
    23161 aacctgcgct ggcaaccttt gccatgccgg atgtgccgcc agcgccaacg ccagctgaac
    23221 ctgccgcgcc tgttgtagcc ccagcaccga aagctgcacc ggcaacacca gcaactcctg
    23281 cacaacctgg gctgttgagc cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg
    23341 aagaaaccaa accgaccgag caaccagcac cgaaagcaga agcgaaaccg gaacgtcaac
    23401 aggatcgtcg caagcctcgt cagaacaacc gccgtgaccg taatgagcgc cgcgacaccc
    23461 gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccaag
    23521 cacagcagca gactgccgag acgcgtgagg gccgtcagca ggctgaggta acggaaaaag
    23581 cgcgtaccgc cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg
    23641 ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg
    23701 aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca
    23761 atcagaaagt gcgttacgag caaagcgtag ccgaagaaac ggtagtcgca ccggtagctg
    23821 aagaaactgt cgctgccgaa ccaattgttc aggaagcgcc agctccacgc acagaactgg
    23881 tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg
    23941 ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc
    24001 gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc caatcgccaa
    24061 tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc tggatccgct
    24121 atccgattgt acgtccacaa gatgtacagg ttgaagagca gcgcgaacag gaagaagtgc
    24181 aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaaccg gttgttagcg
    24241 cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc
    24301 aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta actgaacagc
    24361 cgcaggtgat taccgagtct gatgttgccg tagcccagga agttgcagaa cacgcagaac
    24421 cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa actgctgagg
    24481 ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg
    24541 cagcagaagt tgaaacggta accgcggtta aaccagagat caccgttgag cataaccacg
    24601 ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca ccgcgtcaca
    24661 gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg
    24721 caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa taattagctc
    24781 aagtaatcaa gccctggtaa ctgccagggc ttttttattt catctttgaa tcaacatatt
    24841 aaaattcatc gaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaatgcccg
    24901 ccccggcgat ccctgagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc
    24961 gagcttattt gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg
    25021 atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat
    25081 caaccagatc gctcatctgc tgcgtttgcc ctaatgcgcg atcgctacct aatgaatcca
    25141 gcgactccag ttcgttcagc tgcgtgccta attccgcgcg aacggtcagg acattgttca
    25201 gcgagttttt cagtccgcgg ttggttttat ctaacgccgc agcggcggtt tctttatccg
    25261 cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa
    25321 gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga
    25381 aaattttgtc gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa
    25441 tactttctgt tccgccgaca taatcaccgt taacctcact aaacggcgca gtctctgttt
    25501 tataaccggc aaaaatgtag cgcccgttac cgtcagtggt gttcgccaga ttcagcaact
    25561 ggtcacgaag cccctgaata tccgtagcca gcgaggcccg gtcattgtca ctcaaggtgc
    25621 cattgctggc gtagacaatt ttttcctgag cattctggat agcggtggtg acctggctaa
    25681 gtacactctc ttccagtgac actttttgag tggcgaaagt acgtgccagc gtgtactggc
    25741 tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg ggatcgtctg
    25801 aagggttaac gactcgctta cccgtcgaca tctgctcgcc gtacttcatc cattctgcct
    25861 gagaattggt gatgccgcgc atgttttgct ggtacatcat ctgggtactg aaacgcattt
    25921 tatctcccct tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg tctgcagaac
    25981 ctgcgcattc gccaggtaat actgctgaaa acgttgcaga tttccgtact cctcatcgag
    26041 attgacaccg gaaatcgact gctgctgatt ggaaagctgc gtcaccacat taccttgcgt
    26101 ggtgctactg gttttcaacg tcgcggtttt attaccgata tcactcacta acgaggcata
    26161 agcgtcgtta aaggatttcg ctccgcccac cgttttactg ttgctttgca gatccagcag
    26221 ggcctgaccg ttgcggttgt cgctatcacc cgcatcttct tcgctcgcca tcgctatttt
    26281 cgcttcgtcg gtgattaata catccatgtt gacgatggcg tcacttactg gtttcagcgt
    26341 gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac tccagaccat caaatgccac
    26401 tttaccgttg gcatctggcg tcaccgtaaa agtggtattg ctggcaaggc gggtgacctg
    26461 ccactgatta ttatcgaacg agattttgta atctgtcgcc agtaccgcgg aggcatcagt
    26521 taccgtggca ccgatcgcaa cgttaccgtt gtttttagtg ttttgcagaa ccgcgggctt
    26581 accgatagca aagaaatctt caccctcatc gccgttggca tcaaatccgg ctttgtgttg
    26641 gctgttgaaa gcctcagcaa atgccagcgc cagttgtcca agcgtattac gcgtctggtc
    26701 cagatcctga gaacggaatg tcagaatgcc gcccagcgac ccggtattca gtaatttctc
    26761 cgggatctca atattgcctg ccgtcccatc aacataagcg acagtcgtac gagaagggtc
    26821 ggcgctggaa ggaactgccg ccagttgccg cgccgtactt ccctgaacca gtgagtaacc
    26881 attggccatc gtgatgttat aagtgccgcc atcctgaacg ctgacttcta caccaacaat
    26941 ctggtttaat tcgctcacca gttgatcgcg ttgatccagc agattgttag gtgacgcccc
    27001 tgcccccacg cctgtcaggc gggagatttg atcgttcagg ctggcgattt gtttagcgta
    27061 attgttgatc tgatcaacgc tggcacctat cgcgatattg acctgtttgt cctggtcgcg
    27121 aagatactga tcggtggttt taaactgatt caccaaccct tctgatttcc caatcagcgc
    27181 ctggcgcgct gccgggtctt ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc
    27241 ctgcatctgt gttgccagcg aagaggtact ggtggagagc atattgtcga ttttcgacat
    27301 ctgctcatag cgggcagtca ggccgctact ttgcgtctgc gccgcacgta actggttggt
    27361 aataaacgca tcatactcac gctgcacacc agaaacgtag acaccattac caacccagcc
    27421 gccagcgccc aacgtgctat tggcctgcgc cataatagtg gtttggcggg tatatccggc
    27481 aacgttatag ctggagatat tattacttgc cgtatttaac gccgcctggg ccgcgttcag
    27541 tccgctcatg gcgttattga tcaagctgga catggaggtt ccttataagc cttcaattac
    27601 agagtattat cggcagcgac ccgccggact tgagttattc agaacagatt atcaatgttc
    27661 atgctgtagg ttttgctcac cttgtcgctt atcgatttca tctgctgaat catgttggtg
    27721 agtttacggg catagtgagg atcggtggca tagcccgcgt cctgtagggc ctgcgccccc
    27781 tgctccgcac tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgtgt taacagccca
    27841 acgtaatccg acaatgcttc cagatacgag ctgtagaccc gaaacttcgc ttttactttc
    27901 ttcgcttcgc cattttcata ttcagtcgtg gtgatttcag tgactggccc tttccagttg
    27961 ccagaggctt tgacaccaaa caggttatag ctcggctcgc cgttttcgcg gcggatttgc
    28021 cgttgccccc agccagattc cagcgccgcc tgagcgagga tcaaatgatg tggcacaccg
    28081 ctttgctggc ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc tttactgtca
    28141 cctggcagcg aatcatcgta gttacgtggc acggcctttt gcaccagctg cgaaagcgcc
    28201 tgattttgat aacgcaccac ggtttcgagc gggaatttca tcggtgctgc tggcgtggac
    28261 tcctctggca atggttgttc tggcgtcatc tgcttaacca tcatctctgc aagccccaga
    28321 cctttgcccg tcgtcatctg ttgggcaatc tgctggtcat acatactggt atacaggcga
    28381 gtgtgctcgc tgctgaacag gccatctttt ggtaacgcgt cgcgcatgct tttcaacatc
    28441 atctgcacga acatcccttc cacctgacgg gccaccggac ggatatttgc cgccggatct
    28501 tcgctcgctt tcgcctttag ttcgttgagt gattgcgcat cccaggccgc acttgccagt
    28561 agtttgctgt cgctgatcat cagatgattt ccagttttgc ccgcagacat cccgcacttt
    28621 gcattgattg caaaatagac atcagatcca tcggcgtagc gcccagcgca ttgagcgcgc
    28681 gcaccacgtt attgaggctg gcgctggaac gtacgctttg cagcgaaccg ccgctctggc
    28741 gtaagtcgat ctgcgtttgt ggcgttacca cggtctgtcc gccaccaaac ggtgtatctg
    28801 gttggctgac attggcctga cgattgactg ttactgagag atttccctgc gctatcgcgc
    28861 agctgtcgag agtcacttcg cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta
    28921 ctttagcgtc ctgcggggtg acattaacct gcatattctg gatatcggca aggaaacgga
    28981 cctgggaact gttgccactc ggtacgcgca cctgaatagt ccgcgcatcc aacgcggtgg
    29041 cgctgccata tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat
    29101 cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc aaactggctg ggtaattcac
    29161 gttcaataac tgcaccattg gtgatccgtc caccgttcag ttggttcacc tgaacactgc
    29221 taccgccagc ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc agcgcataca
    29281 cctgactgtc aacgccctta agcggagtca tcaacaatgt gccgccacgc aggcttttgg
    29341 catttcccat ggaagaaacc accacgtcaa tggtttgccc ctgacgtcca aacggtggaa
    29401 gtgacgccgt caccattacc gcagcaacgt tttttagctg catattggtg cccgtcggaa
    29461 cggtaattcc cagctgtgag agcatgttat taagcgtttg tgtggtaaac ggcgtctggg
    29521 ttgtctggtc accggtgcca tccagcccca ccaccagacc atagccaatc agtgagtttt
    29581 gccttacccc ctgaacactg gtgagatcgc gaatacgctc agcctgagcc gccgtcgtaa
    29641 ccagtagaag aattaatgca gagagaaatt taatcaccac agcctcactt acattggcga
    29701 caggttaagg aagaaacgct gcaaccagcc catattttgc gcttcgttaa tgtagccatt
    29761 gcctacgtat tcaatgcgcg catccgccac ctgagtagac ggtacggtat tgctgccgct
    29821 gatagtgcgt ggattaacca cgccagagaa gcgaataaat tcggtaccct gattaatggc
    29881 aatctgtttt tcacccacca catgcaggtt gccgttgacc agtacctggt caaccgtcac
    29941 cgtcaacgtg ccgctaaagg tattactggc attggccccg ccttttccgt tgaacgtgtt
    30001 accaccggag gcttcgacat cggcacgagc gttaccaaac agcccctgca aatagcgcgg
    30061 cacagtatca aagccaaaat tagttttacc gtcacggctg gcattcgcag aggagctttt
    30121 gctggcgcta acgttctcct gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg
    30181 acgatcttca aacagcggtt gatagccata gttaatcggc tgagcagact ggaaaataga
    30241 accgttggcg acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc cctgcaccag
    30301 cggcgtggag ggtatccagg cgcagccggt tagtgaaagc accaacaagc tggaaatggc
    30361 ataagtatgc gcagcgtttt tttgcatggc tatcatcttc aaaaatcaat aaaccggtga
    30421 acctgccacc ggttatgcct tagagttgcg tcagtttttg cagcatctga tcggtggtgg
    30481 acaccgcttt actgttgatt tcgtaagcgc gttgcacctg aatcatattg accagttctt
    30541 ccgccacgtt gacgttagac gtttcaacat acccttgata cagcagtccc gcgccgttca
    30601 ggcccggcgt gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag aggttttcgc
    30661 caatgctctc cagcccggtg tcattcataa aggtggtgag attgagctgc ccaacctgaa
    30721 ccggagctgc ctggccttgt tgggttacgc tgaccacgcc atcacgaccg atggtgatac
    30781 ttaacgcatt cgccggaatg gtgatcgctg gctgcacctg aaaaccacca gccgtcacca
    30841 gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt ataggctgat gagccatctg
    30901 gcaacatcac ctggaaaaag ccctgccctt taatcgcgac atctttgctg ttgttggtct
    30961 gcgacaggtt gccttggctg tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga
    31021 tttgtaatcc ggagggtaag gtggtttgtt cggaagactg tgcccccggc tggcgaatgg
    31081 tttgataaag cagatcttca aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt
    31141 ttgccaggtt gttggcaatg acgtccatat tggtttgctg ggcgtcaagg cccgttttgg
    31201 cgatccataa tgaactgatc ataaaatgtc ctgtagtgga ttgctgacaa gcagagcaga
    31261 agcacgcggc ggtgcgaatt tcccctcacc ctaaccctct ccccaaaggg gcgagggaac
    31321 agtccgctcg cttttattgc aatgtcatca atcgttcaat taactcatcg acagcagttg
    31381 gttggcacgg cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg
    31441 ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag
    31501 aacccccgac atcacacgca aggttggatc tgcctgcagt accggtccac gcgtggcctg
    31561 ggtttctgcg cttaaacgaa aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc
    31621 tttcaccagt ttcagacgcc ccactggcgc aaccgtattt gccggatcgc ccggattcag
    31681 cgccgagatt gttccatcgg cagctatcgt gatttccgcc ccttccggca cagcaattgg
    31741 ccccgcctcg cctatcaccg gatgcccctg aattgtcagt tgcccggtgg gatcaacctg
    31801 aatgctgcca ttacgcgtat acccttcgct gccgtcagcg gtctgcacgg ccaaccagcc
    31861 atcctgctgc aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc cgggcgttat
    31921 gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac
    31981 cggcactgcg cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag
    32041 attactggcg gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcggtata
    32101 aattgcgtga tccattgagc tatcccgtca gcgattagcg taagttaacc agcgtgttga
    32161 ggatctggtc ctgggttttg atggtctggg cgttagactg atagttacgc tgggcaacga
    32221 tcatattgac cagttcttta ctgagatcga cgttggacgc ttccaacgca ccgttggtca
    32281 gggtgccaaa gttgcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg
    32341 cagaccagac gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta
    32401 caatctgccc cagcagttgg gtttgttcgt tggaatagtt gccgacaacc gtaccgtcat
    32461 cattgatttg ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa
    32521 tgttgttagc gccggtattt tgctgcatgg agttgaggaa gctcaggcta aacgtggcgg
    32581 gttctgcgcc gttaattgcg ccggtggtaa tattctctgt tggatttgag gtcagaacgc
    32641 cattggcatt aaacaccagc ttcattgcag gctcggctgt acctgttgga tcactgctat
    32701 cctgggtgta gacctgccag ttattatccc cggtcttcac aaagtagacg ctcatgtcat
    32761 gagcattacc ctgactgtcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat
    32821 tgctggcatc aaatgcgtta acagagggaa gcggatcgct ggaattcagg ttgatctgca
    32881 tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atgttagtcg
    32941 gattcgcccc ttgctgaata gtcggcggcg taccggttgc cgggtaaccc gtcagctgta
    33001 aaccttgcat attcaccagg ttacgatttt catccagctt aaattgtccg ttacggctgt
    33061 agaacaccga accgttgcta tctaccagac ggaaaaaacc gttctggctg atagcaacgt
    33121 ccagaccacg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa
    33181 cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg
    33241 atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg
    33301 cggcagcgtt taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga
    33361 aggcttaaat tatctgccgt acttcgtcga gggtggtggt gccgtaagtg ccgagatcca
    33421 gcgtattacc gttgttgccg cggatcacac cctgcaccag agcaaactgc agcggctggg
    33481 caaccagttg tgtaccaccg ttactggcgc taatcgctac gttgtaagaa ccgttcggcg
    33541 cagtgctgcc gtcagtcaac gtaccgtccc aggtgaaact gtgaactccg gcggtcagtt
    33601 caccaatatc aatggtgcgc acaaccgcgc catttttatc ggtgatggtg gccgtaactt
    33661 tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca gccccttctt
    33721 cactgccggt tcccgcaaga acagtggtgc cggggatcat cacgccgtga ccgatcaggt
    33781 tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatccg agcgtggtat
    33841 tgagtttttc aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt
    33901 tttccattgg attggtcggg tcctggtttt tcagctgcgc caccaacaaa gtcagaaaac
    33961 tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc
    34021 cggtatttgt cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc
    34081 gagcgtaagg gttttcagca tcatgctttt caccgtgttg agcacttcaa cattggcctg
    34141 atagctgcgt gacgccgaca tggtgttaac catctctccg acaacatcaa cgttcggcat
    34201 ttttacgtag ccctttgcat ccgccagcgg attacccggt tcataaacca gtttgtccgg
    34261 ggcctgactt tctataacat cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc
    34321 gttaacctgg aataccacct gttttgcccg atatggctgt ccatcaggac cggtcacgct
    34381 atcagcattc gccagattac tggccgccac gttcaggcgc tgggactggg cagttaacgc
    34441 cgacccggcg atatcaaaaa tattcagcag tgccatccgt taatttccgc tctgtaaaac
    34501 gttcatcatg cctttgattt gcccgctcaa cgcgctaagg ctcatctggt attgcaggct
    34561 gttatcggca aactgggtgc gttcgcgatc catatcgacg gtattaccgt caagcgaagg
    34621 ctggtccgga atacggtatt gcagttctgc ggtaggaggc gtcagcgcct gcgccggaat
    34681 gtgttgcgtt gaggtcatcg tcagagcaac cacactggtt gcattccgtc cacgttgcat
    34741 gactttttta agttcactgg caaaatcgat atcgcgcgcc tgataaccag gggtatcggc
    34801 attggcgatg tttgctgcca gcacttcctg acgctgggcg cgcagattga gcgcctcttg
    34861 ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct cctccgcagg tatcaaaatt
    34921 ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc ggaataaacg caaaatgggt
    34981 cgctatttat gccgttgatg gtcattgtgg acaggtacaa ttcacgttgt agaaatggct
    35041 gggggcgaaa atgctggcaa taaaacgtag cgtggcgatc atcgcgatac tgttcagtcc
    35101 gttaagtgcg gcgagcaatc tcacatcgca attgcaaacc ttttttagct cccagctcgc
    35161 agggataagt gatgaggttc gtgtttctat tcgtacagcg cccaatctac taccgccgtg
    35221 cgagcagcca ttgctttcga tgagcaataa ttcccgcttg tggggcaatg tgaatgtgtt
    35281 ggcacgctgc ggtaacgaca aacgatattt acaggttaat gtgcaggcca caggaaatta
    35341 tgtggttgcc gcgatgccca ttgtgcgagg aggaaagctg ggagctggca atgtcaaact
    35401 gaaacgcgga cggctggata ccttgccacc gcgtacggtg ctggatatca atcaacttgt
    35461 tgatgccgtt agcctgcgcg atctatcacc cgatcaacct atccagttaa cccagtttcg
    35521 ccaggcatgg cgggtaaaag cgggacaacg cgtcaacgtg atcgccagcg gtgatgggtt
    35581 tagcgccaac gcagaaggtc aggcgctaaa caatgcagcc gtcgcacaga atgcgcgggt
    35641 gcgcatggta tctggacagg tagtcagcgg cgttgttgat gcagatggga atattcttat
    35701 aaacctgtaa gctgttaaag attacccgtc ccttgccgat aaataagcaa cacatgataa
    35761 aagcgccctc aatgaggaat aaaccatgag tattgatcgc acttcgcctc tgaagcccgt
    35821 aagcaccgtt caaccgcgcg aaaccactga cgcgccggta acgaacaccc gagcggcaaa
    35881 aacaacagcc tccaccagca ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca
    35941 acccggcagc agtgatatca atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa
    36001 cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca
    36061 agacttgcag agtaattaat cgtatgacgc gtcttgcaga gatcctcgac cagatgtccg
    36121 ctgtgcttaa cgatctcaaa acggtaatgg atcaagagca gcaacatctc tctatggggc
    36181 agatcaacgg cagccagttg caatggatta cagaacaaaa aagctcactg ctggcaacgt
    36241 tggattacct cgaacagtta cgcaggaaag aacccaatac agcaaatagc gttgatatta
    36301 gtcaacgctg gcaggaaatt gctgtgaaaa cgcagcaact acgccaaatg aatcaacata
    36361 acggctggtt actggaagga cagattgagc gcaatcaaca ggcgctggaa atgttgaaac
    36421 cgcatcagga accgacgcta tatggggcga acggtcagac ctcaacaacc catcgcggca
    36481 gtaaaaagat ttcgatctga agattatctc cggcctgcac tgcaggccgg aatgcattgt
    36541 tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca gtaccgccag tgcagcgaag
    36601 tacgcggcaa tccccgccag cacgaccgcc attaaacgca gcaaacgcca gggcatggta
    36661 cccaatgacc actccggcat gatatgtaac atacctaaaa gcacgccaga catcaccagt
    36721 accgccacca ccagacgcaa cagaaacgcc atccagccag gttgcggggt aaagattttc
    36781 tgcttacgca actgccagta aagcagcgaa gcattcagac acgccgccag accaatagaa
    36841 agtgacagcc cggcatgttt caacggaccg ataaacgcca ggttcatcaa ttgcgttaaa
    36901 atcagcgtaa cgatggcaat tttcactggc gttttaatgt cctggcggga ataaaagcca
    36961 ggagccaaca ctttcactac aatcaggcca atcaaaccca ccgagtaggc aattaacgcc
    37021 cgctgggtca tcagcgcatc aaaggcggta aatttaccgt actggaacag cgaaacggtt
    37081 aacggaccgg aaagaatgcc caacgcaacc gcactcggca acgccaacag gaaacaaaga
    37141 cgcagtcccc agtccatcaa acggttgtat tcatcatgat tgccactggc aaaacttttc
    37201 gacagcgacg gtagcaaaat ggtgccaagc gccacgccca gcacaccgga cggaaactcc
    37261 attaagcggt cggcgtaata catccatgac accgaaccgg aagcaagaaa cgaggcaaaa
    37321 atggtgttga tgattaagga gatctggctc acagagacgc caaggattgc cggtcccatc
    37381 tgtttcacca cacgcattgc tccggcatcg tggaagttaa tgcgtggcaa aaccagcatg
    37441 ccgatcttct tcagatgcgg tagctgatac accagctgca ggacgccgcc gaccgtcaca
    37501 gcccacgcca gcgccagcac cggtgggtta aagtacggtg cggcaaacag cgcgaaacca
    37561 atcatgctga tgttaagcag tgttggagca aacgccggaa tcgagaagcg gttccacgta
    37621 ttcagaatcg ctcccaccag cgacgccagg gagatcagca agatataggg aaaggtaatc
    37681 tttagtagct ggctggtcag ggcaaatttg tcagctgtgt cagcgaagcc tggcgcggtc
    37741 accatgatca cccacggtgc ggcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt
    37801 gtcagcaggc cagaaacata agagacaaag acccgcgtgg cgtcttcacc ctgcttactt
    37861 ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg
    37921 cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca
    37981 aagattctgg cgacaattgc gtctcgtgcg aagccaagca cacgcgaaaa catggtcatc
    38041 gagctgacgg cggccagcga ttttaataaa ttcatcggtg ttctaatcca gacccacggg
    38101 caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt ctacctggat tatggtgaat
    38161 tgctaccgcc agatgttaca gggtttattc actcatcgca tcgcgccaga tcttgtcaac
    38221 gatacgttgc gccagcactg cctgttcgcc ggcggtttgc ggaactgtct gattttgcac
    38281 gcattcaatg aagtgccgcg cacagccgac aaatccacgt tgctcaagcg tgctctgcca
    38341 accaggaatc ggtttattca ctacaccctg cccacgctcc tcacgccatt cgcgcatttc
    38401 cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg gtttcacgct gacttccggc
    38461 acggcgatgc atacaagtgg tgatctgcaa aggaccggcc gaaaaatggt gctcggcaaa
    38521 cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca tccagagagg ctttgccgcc
    38581 cgacaaccac agcgcggtat ccaccacatg cagataatca tccagcaacg tgaaataaag
    38641 atcgtgtggc ccgacgctat tgctacgatg tttatccatt cttagcgagg ctgcggtggc
    38701 gagttgcgtt tttaactcac agtagagtgg tgcgaaacga cggttaaaac cgaccatcaa
    38761 cgtcagtttt ttccgcgccg ccagttccac cagccgttca gcatcgcgca gattttctgc
    38821 cagcggttta tcgacacaga catgtacccc cgcattgagt aacgtactga ccacgtcaaa
    38881 gtgactggcg gtgctggaat gtacaaaaac cgcatcgcaa ctggcggcaa ggctggataa
    38941 cgaatcggca taaggaatgc gccagctttc acaaattggc agggctttcg cgcgcgtagg
    39001 cgaccaggct ccttgtaacg tccagtcaga cgctgccgcc agcaccggta accacgcttt
    39061 ttgcgcaatg ccgcctaatc ccactacgcc gatacgtaat tttttcactt taatctccca
    39121 ggtgggccag caacgaatca agacgctgtt tcagttctgc cacttcgatt tccagggctt
    39181 cgacgcgggc ctgtaaatca ccgtcaaccg cgttcgacat atccgttacc gccggctgat
    39241 cctctacctc accactgaaa agatgcatgt agcggctttc gcgtttaccc ggttcgcggg
    39301 ccagacgcac cacaaaagga ccatcttcgc gattcgccag ttgttccagc gtcaactcca
    39361 cttccgccat atcgctgaat tcatacattc gcgcggcgcg gctgcgcagt tcacccggcg
    39421 tctgggcacc acgcaataac aacgtggtga ttaacgccac ttctgcggcg ctcagtttca
    39481 gatcgccaaa ttctgaatta caaaaacgtt gctcatattt ggtgacccga ttaccaaaac
    39541 cgctcactgt gcgtagataa tgacgtttga ccagattatc cagctgttcc tgcacttcag
    39601 attcactcag attcatgacc ggttcacggt tcgttttctg attacaggcc gtgactacac
    39661 cattgactga gagcgggtat tgctccggcg tggtcacctg cttttccagc aagcaaccaa
    39721 tcacacgagc ttccagggca gtaagttgat atttcatgag attctcctta gcggccgggc
    39781 gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac
    39841 agatagtctt tcgcatagcc ttctttttca aaacccagtc gcgccagtaa atcaccgctg
    39901 cgtttattgt gcggcatata attagccata atgcgatgaa tatgttgggt gcgctgcata
    39961 taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt tgccctgcca tttttgccca
    40021 atcgaataac cgagatagca ggcatgaaaa gagccgcgaa caacattgga aaaattggca
    40081 acgccaataa tctctttttc gtccgggtcg aataagccaa agtagaaagc tgaaccttgt
    40141 ttatgaaatt cgttaatcat ccccagcctg gcctgccagc ctgatggata acagtggctt
    40201 tcgtcgcgca ctggctccca gggcttgagg aaatggcgat tctctgcgta ataatccgcc
    40261 agacgccagg catcacgatc atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc
    40321 acttttggca cgttactgcg atagccaaac attctatacc tactccttcc cgtaacgtct
    40381 ttaatacgct gtaagtatta ctatacctgc gcaaccagac cctgtgaaaa gtccaaaatg
    40441 ccattttctg cattattcac atatttgata aaaactctct atcaaagcga cgttttgata
    40501 aaaaaatatt gtccccgaag tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg
    40561 ctcatctttt atttcccggg aggggaaatg tcccgcgtgt cgcaggcgag gaacctgggt
    40621 aaatatttcc tgctcatcga taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg
    40681 ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg ccgtcatggt cggtattgcc
    40741 ctcggcctac gccagtttat tcagcaaggt ctgggtattt tcggcggcgc aattgccgac
    40801 cgctttggtg ccaaaccgat gattgttacc ggtatgctga tgcgtgccgc cggattcgcc
    40861 acaatgggta tcgcccacga accgtggcta ttgtggtttt catgcctgct ctcgggactc
    40921 ggtggcacgt tgtttgatcc gccgcgttcg gcgctggtgg tgaaactaat ccgtccacaa
    40981 cagcgtggtc gttttttctc gctgttgatg atgcaggaca gtgccggtgc ggtcattggc
    41041 gcattgttgg ggagctggct gttgcaatac gactttcgcc tggtctgcgc cacaggggcc
    41101 gttttgtttg tgctgtgtgc agcgttcaat gcgtggttgt taccggcatg gaaactctcc
    41161 accgtacgca cgcccgttcg cgaaggcatg acccgcgtga tgcgcgacaa gcgttttgtc
    41221 acctatgtcc tgacgctggc gggttactac atgctggctg tacaagtgat gctgatgctg
    41281 ccaattatgg tcaacgatgt ggctggcgcg ccctctgccg ttaaatggat gtatgccatt
    41341 gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc gctggagtga aaagcatttt
    41401 cgtctggaac accggttgat ggctgggctg ttgataatgt cattaagcat gatgccggtg
    41461 ggcatggtca gcggcctgca acaacttttc accctgattt gtctgtttta tatcgggtcg
    41521 atcattgccg agcctgcgcg tgaaacctta agtgcttcgc tggcggacgc aagagctcgc
    41581 ggcagctata tggggtttag ccgtctgggt ctggcgattg gcggcgctat tggttatatc
    41641 ggtggcggct ggctgtttga cctgggcaaa tcggtgcacc agccagagct tccgtggatg
    41701 atgctgggca ttattggcat cttcactttc cttgcgctgg gttggcagtt tagccagaaa
    41761 cgcgccgcgc gtcgtttgct tgaacgcgac gcctgatttg atactccccc ctgactcctc
    41821 catactgaaa gtgaactgac agccaaatgg aggagtcacg tgaagctata catttacgat
    41881 cactgccctt actgcctcaa agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa
    41941 ttacatgttc tgctcaacga cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag
    42001 gttcccattc tgcaaaaaga tgacagccgc tatatgccag aaagcatgga tatcgttcac
    42061 tatgtcgata aactcgacgg caaaccgtta ctgaccggca aacgttcccc tgccattgaa
    42121 gagtggctgc gcaaggtcaa tggctacgcc aacaaactgc tgttgccgcg ttttgccaaa
    42181 tcggcatttg atgagttttc tactcccgcc gcgcgcaaat atttcgtcga caagaaagag
    42241 gccagcgcgg gtaattttgc cgacctgctg gcccactctg acggtctgat taagaatatc
    42301 agcgatgatt tacgtgcgct ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa
    42361 ctttcggaag atgatattca gctattcccg ctactgcgta atctgacgct ggtagccgga
    42421 attaactggc caagccgcgt tgctgattac cgcgataata tggcgaaaca gacacaaatc
    42481 aatttgttat catcaatggc gatttaatcc tgactcgccg cggcagcctg tattcgcggg
    42541 ttgccgccgt tttcagtttt ttctgctggc gagtttatcg ctggtgacac atgctgcgtt
    42601 gtgtcaggat attgacgcag atttcatgtc gaggactacc atgaacaagt ttttatttgc
    42661 cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat caactcaccc aatacaccat
    42721 caccgaacaa gaaattaacc agtcgcttgc gaaacataat aatttctcaa aagatattgg
    42781 tttacccggt gtggctgacg cccatattgt tctgacaaac ctgaccagcc aaattggtcg
    42841 cgaagagccg aataaggttg ccctaaccgg agacgccaat ctggacatga actccctgtt
    42901 cggtagccag aaagcgacca tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga
    42961 aaaaggcgcg atcttcctga aagagatgga ggtggtcgat gcgacggtac aaccggaaaa
    43021 aatgcaaacg gtgatgcaaa cgttgcttcc ctatctgaac caggcattac gcaattactt
    43081 taaccagcaa cctgcttacg tcctgcgcga agatggcagc cagggcgaag caatggcgaa
    43141 aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt gtcattccat ttactgatta
    43201 atcacgaggg cgcattcgcg ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc
    43261 ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag agattaatga ctgcaccatc
    43321 ccaggtatta aagatccgcc gcccagacga ctggcacctt cacctccgcg atggcgacat
    43381 gttaaaaact gtcgtgccgt ataccagcga aatttatgga cgggctatcg taatgcccaa
    43441 tctggctccg cccgtgacca ccgttgaggc tgccgtggcg tatcgccaac gtattcttca
    43501 cgccgtacct gccgggcacg atttcacccc attgatgacc tgttatttaa cagattcgct
    43561 ggatcctaat gagctggagc gcggatttaa cgaaggcgtg ttcaccgctg caaaacttta
    43621 cccggcaaac gcaaccacta actccagcca cggcgtgacg tcagttgacg caatcatgcc
    43681 ggtattagag cgcatggaaa aaatcggtat gccgctactg gtgcatggtg aagtgacaca
    43741 tgcagatatc gacatttttg atcgtgaagc gcgctttata gaaagcgtga tggaacctct
    43801 gcgccagcgc ctgaccgcgc tgaaagtcgt ttttgagcac atcaccacca aagacgcagc
    43861 cgactatgtc cgtgacggaa atgaacggct ggctgctacc atcactccgc aacatctgat
    43921 gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg cacctgtatt gtctacccat
    43981 cctcaaacgc aatattcacc aacaggcatt gcgtgaacta gtcgccagcg gttttaatcg
    44041 agtattcctc ggtacggatt ctgcgccaca tgcacgtcat cgcaaagaga gcagctgcgg
    44101 ctgcgcgggc tgcttcaacg ccccaaccgc gctgggaagt tacgctaccg tctttgaaga
    44161 gatgaatgct ctgcagcact ttgaagcatt ctgttctgta aacggcccgc agttctatgg
    44221 gttaccggtc aacgacacat tcatcgaact ggtacgtgaa gagcatcagg ttgctgaaag
    44281 catcgcactg actgatgaca cgctggtgcc attcctcgcc ggggaaacgg tacgctggtc
    44341 tgttaaacaa taaaaatcg
//
LOCUS       DBFIFS010000012        43516 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.012, whole genome shotgun sequence.
ACCESSION   DBFIFS010000012 DBFIFS010000000
VERSION     DBFIFS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.012"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            228..1445
                     /gene="alaA"
                     /locus_tag="N7A07_000660"
     CDS             228..1445
                     /gene="alaA"
                     /locus_tag="N7A07_000660"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU2475388.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1529..2128
                     /gene="yfbR"
                     /locus_tag="N7A07_000661"
     CDS             1529..2128
                     /gene="yfbR"
                     /locus_tag="N7A07_000661"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU2475389.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2187..4019)
                     /locus_tag="N7A07_000662"
     CDS             complement(2187..4019)
                     /locus_tag="N7A07_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU2475390.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4106..4756)
                     /gene="hxpA"
                     /locus_tag="N7A07_000663"
     CDS             complement(4106..4756)
                     /gene="hxpA"
                     /locus_tag="N7A07_000663"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU2475391.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4767..5261)
                     /locus_tag="N7A07_000664"
     CDS             complement(4767..5261)
                     /locus_tag="N7A07_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU2475392.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5344..5799)
                     /gene="yfbV"
                     /locus_tag="N7A07_000665"
     CDS             complement(5344..5799)
                     /gene="yfbV"
                     /locus_tag="N7A07_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU2475393.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6137..7339
                     /gene="ackA"
                     /locus_tag="N7A07_000666"
     CDS             6137..7339
                     /gene="ackA"
                     /locus_tag="N7A07_000666"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU2475394.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7414..9558
                     /gene="pta"
                     /locus_tag="N7A07_000667"
     CDS             7414..9558
                     /gene="pta"
                     /locus_tag="N7A07_000667"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU2475395.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9748..11268
                     /gene="yfcC"
                     /locus_tag="N7A07_000668"
     CDS             9748..11268
                     /gene="yfcC"
                     /locus_tag="N7A07_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU2475396.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11301..11843)
                     /gene="yfcD"
                     /locus_tag="N7A07_000669"
     CDS             complement(11301..11843)
                     /gene="yfcD"
                     /locus_tag="N7A07_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU2475397.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11901..12452)
                     /gene="yfcE"
                     /locus_tag="N7A07_000670"
     CDS             complement(11901..12452)
                     /gene="yfcE"
                     /locus_tag="N7A07_000670"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU2475398.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12508..13152)
                     /gene="yfcF"
                     /locus_tag="N7A07_000671"
     CDS             complement(12508..13152)
                     /gene="yfcF"
                     /locus_tag="N7A07_000671"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU2475399.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13288..13935
                     /gene="yfcG"
                     /locus_tag="N7A07_000672"
     CDS             13288..13935
                     /gene="yfcG"
                     /locus_tag="N7A07_000672"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU2475400.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13992..14354
                     /gene="folX"
                     /locus_tag="N7A07_000673"
     CDS             13992..14354
                     /gene="folX"
                     /locus_tag="N7A07_000673"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU2475401.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14375..15268
                     /locus_tag="N7A07_000674"
     CDS             14375..15268
                     /locus_tag="N7A07_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU2475402.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15316..16206)
                     /gene="rpnB"
                     /locus_tag="N7A07_000675"
     CDS             complement(15316..16206)
                     /gene="rpnB"
                     /locus_tag="N7A07_000675"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU2475403.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16403..17176)
                     /gene="hisP"
                     /locus_tag="N7A07_000676"
     CDS             complement(16403..17176)
                     /gene="hisP"
                     /locus_tag="N7A07_000676"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU2475404.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17184..17900)
                     /locus_tag="N7A07_000677"
     CDS             complement(17184..17900)
                     /locus_tag="N7A07_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2475405.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17897..18583)
                     /gene="hisQ"
                     /locus_tag="N7A07_000678"
     CDS             complement(17897..18583)
                     /gene="hisQ"
                     /locus_tag="N7A07_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU2475406.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18673..19455)
                     /gene="hisJ"
                     /locus_tag="N7A07_000679"
     CDS             complement(18673..19455)
                     /gene="hisJ"
                     /locus_tag="N7A07_000679"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU2475407.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19676..20458)
                     /gene="argT"
                     /locus_tag="N7A07_000680"
     CDS             complement(19676..20458)
                     /gene="argT"
                     /locus_tag="N7A07_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU2475408.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20724..21293)
                     /gene="ubiX"
                     /locus_tag="N7A07_000681"
     CDS             complement(20724..21293)
                     /gene="ubiX"
                     /locus_tag="N7A07_000681"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU2475409.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21388..22905)
                     /gene="purF"
                     /locus_tag="N7A07_000682"
     CDS             complement(21388..22905)
                     /gene="purF"
                     /locus_tag="N7A07_000682"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU2475410.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22942..23430)
                     /gene="cvpA"
                     /locus_tag="N7A07_000683"
     CDS             complement(22942..23430)
                     /gene="cvpA"
                     /locus_tag="N7A07_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU2475411.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23962..24624)
                     /gene="dedD"
                     /locus_tag="N7A07_000684"
     CDS             complement(23962..24624)
                     /gene="dedD"
                     /locus_tag="N7A07_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU2475412.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24614..25882)
                     /gene="folC"
                     /locus_tag="N7A07_000685"
     CDS             complement(24614..25882)
                     /gene="folC"
                     /locus_tag="N7A07_000685"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU2475413.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25952..26866)
                     /gene="accD"
                     /locus_tag="N7A07_000686"
     CDS             complement(25952..26866)
                     /gene="accD"
                     /locus_tag="N7A07_000686"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU2475414.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27022..27681)
                     /locus_tag="N7A07_000687"
     CDS             complement(27022..27681)
                     /locus_tag="N7A07_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2475415.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27764..28576)
                     /gene="truA"
                     /locus_tag="N7A07_000688"
     CDS             complement(27764..28576)
                     /gene="truA"
                     /locus_tag="N7A07_000688"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU2475416.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28576..29589)
                     /locus_tag="N7A07_000689"
     CDS             complement(28576..29589)
                     /locus_tag="N7A07_000689"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU2475417.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29655..30791)
                     /gene="pdxB"
                     /locus_tag="N7A07_000690"
     CDS             complement(29655..30791)
                     /gene="pdxB"
                     /locus_tag="N7A07_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU2475418.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30890..31885
                     /gene="flk"
                     /locus_tag="N7A07_000691"
     CDS             30890..31885
                     /gene="flk"
                     /locus_tag="N7A07_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU2475419.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQSEAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31882..33060)
                     /locus_tag="N7A07_000692"
     CDS             complement(31882..33060)
                     /locus_tag="N7A07_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2475420.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33335..34555)
                     /gene="fabB"
                     /locus_tag="N7A07_000693"
     CDS             complement(33335..34555)
                     /gene="fabB"
                     /locus_tag="N7A07_000693"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU2475421.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34714..36720
                     /gene="mnmC"
                     /locus_tag="N7A07_000694"
     CDS             34714..36720
                     /gene="mnmC"
                     /locus_tag="N7A07_000694"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU2475422.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36841..37119)
                     /locus_tag="N7A07_000695"
     CDS             complement(36841..37119)
                     /locus_tag="N7A07_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU2475423.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37153..37701)
                     /gene="epmC"
                     /locus_tag="N7A07_000696"
     CDS             complement(37153..37701)
                     /gene="epmC"
                     /locus_tag="N7A07_000696"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU2475424.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37701..38510)
                     /locus_tag="N7A07_000697"
     CDS             complement(37701..38510)
                     /locus_tag="N7A07_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU2475425.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38510..39334)
                     /gene="mepA"
                     /locus_tag="N7A07_000698"
     CDS             complement(38510..39334)
                     /gene="mepA"
                     /locus_tag="N7A07_000698"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU2475426.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39338..40423)
                     /gene="aroC"
                     /locus_tag="N7A07_000699"
     CDS             complement(39338..40423)
                     /gene="aroC"
                     /locus_tag="N7A07_000699"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU2475427.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40458..41390)
                     /gene="prmB"
                     /locus_tag="N7A07_000700"
     CDS             complement(40458..41390)
                     /gene="prmB"
                     /locus_tag="N7A07_000700"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU2475428.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41556..42107
                     /gene="smrB"
                     /locus_tag="N7A07_000701"
     CDS             41556..42107
                     /gene="smrB"
                     /locus_tag="N7A07_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU2475429.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42278..43120)
                     /locus_tag="N7A07_000702"
     CDS             complement(42278..43120)
                     /locus_tag="N7A07_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU2475430.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43122..>43516)
                     /locus_tag="N7A07_000703"
     CDS             complement(43122..>43516)
                     /locus_tag="N7A07_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2475431.1"
                     /translation="NLLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKV
                     TLMGTEDTDLPGFLSIDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNF
                     NAWLQTVNGRNVTSGDFTATMTVTFEYF"
BASE COUNT        10768 a        11749 c        10857 g        10142 t
ORIGIN      
        1 ccagacttac aattaagaat cagaacaatt cactatataa cattgcatgt aaagcatatg
       61 cacctcatta ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
      121 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
      181 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
      241 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
      301 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
      361 cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
      421 ctcaagggta ttgcgattcc aaaggtcttt actccgcgcg taaagccatc atgcagcact
      481 atcaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
      541 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
      601 ctgcaccaga ttacccacta tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
      661 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
      721 ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa taacccaacc ggcgcggtat
      781 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
      841 tcgccgatga aatttatgac aaaattctct acgacgacgc tgagcatcac tcaattgcgc
      901 cgctggcacc tgacctgctg accattacct ttaatggact gtcgaaaacg taccgcgttg
      961 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
     1021 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
     1081 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
     1141 gtctttatga gcagcgtaac cgcgcgtggg aattgatcaa cgatattccg ggcgtttcct
     1201 gcgtgaaacc ttgtggtgcg ctgtacatgt tcccgaaaat cgacgccaaa cgctttaaca
     1261 ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
     1321 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
     1381 tcgatgatat cgagctgtcg ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
     1441 tgtaatctta atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc
     1501 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
     1561 ccgcctgaaa ctcattaacc gctggccgct catgcgcaac gtgcggacgg aaaatgtgtc
     1621 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
     1681 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
     1741 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca
     1801 ggagtacaag gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga
     1861 gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
     1921 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
     1981 gggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgccg
     2041 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
     2101 tgagattagc caggattcac cgctgtaagc agctggagtc tgcgtcgcat caggcaataa
     2161 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
     2221 caaaccgcca tcacgataat ggtgaacggt acccccagct tcacaaagtc actgaagctg
     2281 taatttcccg gacctaaaac caatgtgtta acaggtgaag aaaccggtgt cataaaggcg
     2341 gcggatgctg ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg
     2401 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc
     2461 ccaataaccg ccgacaagac aaacaaacaa cccagcatca tatgtggccc gtaaccaccg
     2521 ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
     2581 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
     2641 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
     2701 ggattaggaa tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca
     2761 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
     2821 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
     2881 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
     2941 cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
     3001 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
     3061 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
     3121 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
     3181 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
     3241 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg accaatcatt
     3301 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
     3361 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
     3421 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
     3481 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
     3541 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt
     3601 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
     3661 ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc
     3721 agcatcaaca aaaccaacat tttgatttca ctgttgcccg caactttgac cagccatgtt
     3781 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
     3841 atcaggacaa cgttaggatc agaaaagcca gaaaatactt ctgggactgt cagcgttccg
     3901 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
     3961 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
     4021 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
     4081 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
     4141 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
     4201 ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag
     4261 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcgccta
     4321 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
     4381 ctggtgcggg aagccccgct attttatggc gcgctcgcgc taccggcatg gagccagaag
     4441 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg
     4501 gaagcgcggt aataccttcg gtttccgtgg cctcgatttg ctccaaacgc gtaaactcgg
     4561 cggcaatatc agcctcggat ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt
     4621 taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
     4681 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
     4741 aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
     4801 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
     4861 cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct
     4921 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
     4981 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
     5041 acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc
     5101 agctcgccaa actcgcgatc cagttcacgc atctgtaatc cataaccccg ctcaatgatt
     5161 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
     5221 tggttagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
     5281 ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
     5341 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcacgct taagcgtgtc
     5401 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
     5461 caatttaccg cgaacttcat aaaaccagtt gaggattgca gggggtaatg gcgtgacaga
     5521 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gggcagtggc
     5581 aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat
     5641 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
     5701 cggggcaagg cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa
     5761 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
     5821 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
     5881 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
     5941 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
     6001 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
     6061 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
     6121 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
     6181 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
     6241 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
     6301 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
     6361 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
     6421 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
     6481 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
     6541 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
     6601 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
     6661 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
     6721 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
     6781 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
     6841 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
     6901 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
     6961 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
     7021 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
     7081 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcaccg gtggtatcgg
     7141 tgaaaatgcc gcgatggtcc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
     7201 agttgatcat gaacgcaatc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
     7261 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
     7321 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
     7381 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
     7441 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
     7501 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
     7561 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
     7621 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
     7681 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
     7741 ctggtcccga cacgtaagca ccaatttgcc cagtctctga actacgaaat cgctaaaacg
     7801 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
     7861 gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc
     7921 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
     7981 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
     8041 caagaatcca gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
     8101 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
     8161 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
     8221 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggttgcc
     8281 gcttgcctgg ctgccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
     8341 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
     8401 tttatggtga acaccaacac ctggcagact tctctgagcc tgcagagctt caacctggaa
     8461 gttccagttg acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc
     8521 aacgctgact ggatcgattc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
     8581 ccagcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg
     8641 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
     8701 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
     8761 gtagaactgg gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
     8821 ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
     8881 ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
     8941 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
     9001 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
     9061 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg cagaacagct ggcagaaatc
     9121 gcgattcagt ccgctgattc cgctgcagcc ttcggtatcg aaccgcgcgt tgctatgctc
     9181 tcctactcta ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
     9241 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
     9301 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
     9361 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
     9421 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac
     9481 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
     9541 tctgcacagc agcagtaacc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc
     9601 ggaaatagtc actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta
     9661 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt
     9721 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga
     9781 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt
     9841 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
     9901 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgccttct gactaacgaa
     9961 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
    10021 ctgatgaact tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc
    10081 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    10141 attgataacg gtatcctggc gcttattcgc catacccgtg ggaatgaaat tctctttatt
    10201 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg ggaagaggcc
    10261 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    10321 accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    10381 ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    10441 cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc
    10501 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    10561 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    10621 gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    10681 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    10741 ttccgcctta acggtatgac ggttaatacc atggcttcat ccttcaccga aggggcgcga
    10801 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt
    10861 aatggtgaag cgggtgatgc cagcgtgtta aatactatcc tcaacagcat tgccaatgcc
    10921 attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt
    10981 aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg
    11041 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggctttcca gtttggtgat
    11101 ggcttcagtc acatcattta cccgacctca gcttcgttaa tggcgacgct cggtgtttgc
    11161 cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt
    11221 atgtccagcg tcgtggtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    11281 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    11341 ttgcgcttca tccacaacgc tagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    11401 gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg
    11461 aagggaccgt gagagacgca gctgaacaat gcgccccaga cacggcaatt tttatcttcg
    11521 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    11581 tcgcgacgcg cggattccag cagttgctca tcggcctgga ctacaccgcc agcggtcgca
    11641 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    11701 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgc
    11761 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    11821 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    11881 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    11941 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg
    12001 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgct
    12061 actggcagat gggtatgacc gtaaaccagc acatcgttct ggtttaaagc aggcagattt
    12121 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    12181 tgccacggcg cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg
    12241 ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    12301 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    12361 agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac
    12421 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    12481 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgctgaaa gtgcaataaa
    12541 acgctggaca gacgctcgct gccactggaa tgttgcataa tccaccaggc attctggcac
    12601 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    12661 ccattcacca aataaattcg gctgacccag tactaacaaa tgttctgcca tcgcgaacag
    12721 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    12781 aacatctgtc ggacgctctt cgcggatggg catcagatcg ctgcgcaacc aggcctgaat
    12841 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    12901 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    12961 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gttgcaaatg
    13021 ttcaccgctg tcgaggtcga tggtcttgat atgaaacaac aggccttttt cctgcaacgc
    13081 cacccaagcg gataacacat aaggggagaa aaaatgggca tctgaccaaa gcgtgatagc
    13141 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    13201 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata attgtggtgt
    13261 tcagatgatt agacaaccgg agctccaatg atcgatctct atttcgcccc gacacccaat
    13321 ggccacaaaa ttacgctgtt tctcgaagaa gcagggctgg attatcgctt gataaaggta
    13381 gacctgggga aaggcggtca gtttcgcccg gaatttttgc tcatttcgcc taacaacaaa
    13441 attccggcaa ttgttgatca ttctccagcc gatggcggcg aaccgctaag cctctttgaa
    13501 tctggtgcca ttttgttgta tctggctgag aaaacggggc tctttttgag tcatgaaacg
    13561 cgtgaacgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    13621 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    13681 cgttatcagg ttgaaactca gcgtctgtac catgtactga acaagcggct ggaaaactcg
    13741 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    13801 gcctggactc gccagcgaat agacctcgct atgtatccgg cagtcaagaa ctggcatgag
    13861 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    13921 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    13981 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttacgta
    14041 catttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    14101 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    14161 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    14221 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    14281 aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa
    14341 gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    14401 gggattaatt ggtcgccatt tgattccacg tttgctggag ctgggccatc aaatcacggt
    14461 aatgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca
    14521 agggcttgcc gatcaaagca acctcaacgg cattgatgcg gtaatcaacc tggccggaga
    14581 accgattgct gataaacgtt ggactcacga gcaaaaagag cgtctctgcc aaagccgctg
    14641 gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccac cgtcggtact
    14701 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    14761 ggaaccgccg cataacaaat ttacccataa actctgcgcc cgctgggaag aaattgcctg
    14821 tcgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcgt accggcgtag tgctggcacc
    14881 ggatggcggt attctcggta aaatgctgcc gccgtttcgt cttggcctgg gcgggccgat
    14941 tggttccggt cggcagtatc tggcctggat tcatatcgat gatatggtca acggcattct
    15001 ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccgatacg
    15061 caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt
    15121 ccccgcaacc gccattcggc tgttaatggg cgaatcttca gtactggtat taggtggaca
    15181 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    15241 agaggcgctg gcggatgtcg ttcgctgatg tggtttactg caaacatccg ccagttaact
    15301 cccggtgtta cagggttagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcag
    15361 cgtcaggatg acttcgtggt caaatccttt ttccagcatc tcctgagcaa tacgcagagc
    15421 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    15481 tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg
    15541 ggcatctccc gtttgtaata cataattaaa cagggctttt agctgtctgt cattagtgtt
    15601 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    15661 atgtttctga attaactcca acagcgccat tttacgatgt tgcataatct cgtcatccgg
    15721 caccacggta atatccacca acggaaaagc cgatgaatat attttacggg caatcgcagg
    15781 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    15841 aaacagcatc gggatcacca atggaagctc tttatagccc acatcaagat ggttttgcat
    15901 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    15961 ctctatcact acataaagat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    16021 ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt taagcgtcgt
    16081 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    16141 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    16201 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca
    16261 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac
    16321 tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca
    16381 gcgttagcca ccggagaggc aattatttca gcgaaccttt aaggaactgt tgcagacgag
    16441 ggctttgcgg gttgccaaat aactgctccg gcgcgccttc ttcctctatt ttcccctgat
    16501 ggaggaaaat gacgtgagta gatacatggc gagcaaagcc catttcgtgg gtcaccacca
    16561 ccatcgtttt tccctcttcc gccagctgct gcataatgcg cagcacttca cccaccagct
    16621 ctgggtcaag ggccgatgtc ggttcatcaa acaacagaac ttccggttcc attgccagcg
    16681 cccgcgctat ggaaacacgc tgttgctgac caccggacag gtgcaccggg tatttcccct
    16741 gcgcacgttc gtctatcccg acttttgcca gatacttcac cgcccgctcg cgcgcttcct
    16801 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat
    16861 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt
    16921 gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg atcgtctggc
    16981 cattgaccac gatcgaccct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg
    17041 tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca
    17101 gtgacacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg ataacgttta
    17161 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    17221 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    17281 gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt
    17341 ttcagcagat ccggcaccgt ggcagtaaat gccaacgcag tagagtgcag catcaggatc
    17401 acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga
    17461 tacattttaa aagtcgagaa gccataggct ctggcggctt caatttcccc atgcggcacc
    17521 gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    17581 agcacggtac agttcaggcc actgcggaaa aaggcgttaa gaaactcggt ccccttaaca
    17641 atctccagcg tatacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    17701 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact ggagacacga
    17761 ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac
    17821 agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat
    17881 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacagagtag cggcgctcaa
    17941 ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg ccacagacga
    18001 tagcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct ttgaccacat
    18061 cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc
    18121 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc
    18181 cacgagtaaa accgaacgcc ctcgccgcct ctatatgtcc tttcggcact gccataaaag
    18241 caccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg ataataccag
    18301 cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagcg
    18361 caatctgcaa accgtagaaa atcagcagca tcaacactaa gtccggcacg ccacgaatca
    18421 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    18481 cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc tccagcgtga
    18541 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    18601 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt
    18661 caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc
    18721 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    18781 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    18841 cgggccaccg aatttgtaat ctttaccgac aggttgtttg aggaaacctt cgctggcagc
    18901 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    18961 ctgcccctga tacgagacga tttcaatgcc ttttggtgcc caatgttcat taccgaacgt
    19021 ctcctgggta gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    19081 ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt
    19141 gaaggctatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    19201 cttcttcgct tttaaggacg ggattaacgc atccagcgga ttttcgacaa acgtacattg
    19261 cgtattgatg cgtttgcata attcctttgc cagatcgata tcgaagccaa ccagttcgcc
    19321 ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg
    19381 cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    19441 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    19501 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    19561 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    19621 aacctggcgt gcaccatgat gatcattcca tcaggtacag cttccctgcg acgtatcagt
    19681 caccgtagac attaaagtca aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac
    19741 gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc
    19801 ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaggcga
    19861 aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcctgcagtg
    19921 cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca
    19981 ccacatccac acctttgcta cgccaggtct cgttagcgta agcttcctgg gttgatccct
    20041 gcagcacacc aacatgttta cctttcagtg agtccagcgt tggctgaatc ggcgaacctt
    20101 tggcggcaat cagacgggaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct
    20161 gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc gatttttttc gctttcagtg
    20221 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    20281 acatctcgtt accgagatcg atatcaaagc cgacaaagtc acctttggca tctttcgacg
    20341 agaacggtgc ataggtggta tcggttccga tacgtaccgt ctccggcaat gccgcatagc
    20401 tggaagccgc tgcggaaata ccgactaaca aagacagagc gagaatcgac ttcttcatac
    20461 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    20521 tgttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt
    20581 aataagacgt tgcattattg tcctaaagtt gaagatagca ggtatagcgg ttgaatcgca
    20641 gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    20701 cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga
    20761 agggttatcg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    20821 gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgct
    20881 gcctgcgtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc
    20941 agtgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    21001 ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat ccccagtgtc
    21061 tggaaagaac cggaagagat gctggcggca atatcgcgcg catcatgcgt gacatcagct
    21121 aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc
    21181 tggctcatta ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc
    21241 acaccataaa tcgcgccgct ggcaccgctg atgcccacaa taagtcgttt cataaatagt
    21301 tgccctgtca gacttgcagg cagactttgc aggatttcgg cgggagttgc aagtcagggt
    21361 gccagaccgg caccctgagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    21421 ttcgttctga cgttgcactg ctttggcgtc gtcattacgt aaagtatcga ggaaatcgag
    21481 gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg
    21541 ctggatatcc gggttttcag cgcgaacggc ttcgatcaga tcgttcagat cctggaaaat
    21601 caacccgtca gcaccgatga tctggcgaat ttcatctact tcgcgaccgt gagcgatcag
    21661 ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc
    21721 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    21781 agtggtgcca cggacgatag agtcgtcgac cagcaggacg tttttatcgc ggaactcggc
    21841 gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctgac ccggcatgat
    21901 gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    21961 acgagcaatt tccagcgcga tatcacacga ggtttccggg atcgggatca ccacgtcgat
    22021 atccagatct tcccattcgc gggcaatttt ctcgcccagc ttcgtgccca tattcacacg
    22081 cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgagcaaagt atacatactc
    22141 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    22201 ttcagtgatg taaatcgctt cgccaggcgc gacgtcacgc aggaaatcaa agcccagtgt
    22261 atcgagcgca acgctttcgg aagcgaccat atattctgtg cggttctcgt caatatcacg
    22321 ttttcccaga accagcggac gaataccgtt tggatcgcgg aaagcaacca taccgtggcc
    22381 gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtag cagcgatggc
    22441 ggcgaaaatg ttgtcggctt caagcgggta gtggcggaag ttgtccagct cgctggcgaa
    22501 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    22561 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    22621 atacggagag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    22681 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    22741 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    22801 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    22861 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    22921 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    22981 atcaaaaaac catctgatga taaaactgaa ctgcggaatc agttgtgatt tactccagtc
    23041 ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatagcag caacaatcaa
    23101 cacaccgcgc aatgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    23161 tttctccacc agcaggccta tcacgaagtt cacgatagcg ccaacgatca gggtggcgat
    23221 aaacaggacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    23281 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    23341 cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac
    23401 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    23461 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    23521 catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg
    23581 catcggacaa tgcgtgtcgg atgcggcgta aacgtcttat ccgacctacg gttctacccc
    23641 tgcgtaggcc tgataagacg caccagcgtc gcatcaggca acggctgtcg gatgcggcgt
    23701 gaacgcctta tccgacctac ggttctaccc ctgcgtaggc ctgataagac gcaccagcgt
    23761 cgcatcaggc aacgcctgtc ggatgcggcg tgaatgcctt atccgaccta cggttctacc
    23821 cctgcgtagg cctgataaga cgcgccagcg tcgcatcagg caacggctgt cggatgcggc
    23881 gtaaacgcct tatccgacct acggttctac ccctgcgtag gcctgataag acgcgccagc
    23941 gtcgcatcag gcaagaccgt attaattcgg cgtatagccc attaccacgc cacttaagcc
    24001 agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg catccggccc
    24061 aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata
    24121 accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcgcc
    24181 cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg
    24241 ttctggcttc ggcgcaggtg gtgcttcaac cttcggctta ggcggctcca ccggtttcgg
    24301 cttcggtggg acgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt
    24361 ggccggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc
    24421 cggcggttgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct catcgcgatc
    24481 gcccgctttt ggcaccagcg ggatagccgc aaactcatcc tgataatgtt ttttctgccc
    24541 gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa
    24601 ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg
    24661 cgaccgtgtg gaacgaacca cacaccagca cggtgtcttc cgctttagcg tccgccattg
    24721 cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca
    24781 gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca
    24841 cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg
    24901 ccagcacgcg cccgtttttc ggtagcgttt tcatacgccc ggtgagatat tccgccgcat
    24961 gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc
    25021 ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgacc tccagcccgc
    25081 tggcacgcag tgccgccagc gccgttgcgg cattcggttg cgggacaagc ggcaacggca
    25141 ggttttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt
    25201 tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg
    25261 tagaaggcat ttccggctca ccgacaattg ccggtttttc gctgcgaaag atgcctgctt
    25321 tctcgcgacc aatactttcg cgatctggcc ccagccagtc ggtatgatcc agcgcaatac
    25381 tggttactac cgcgacatcg gcgtcgacaa tattggttgc gtccagacga ccgcccagcc
    25441 ctacttccag aatcaccacg tcaagttgtg cctgtttgaa cagccacaac gccgacagcg
    25501 taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag
    25561 aggcggtgtg ggccgattcc ggcaattcct gaccctgcac acgtacgcgc tcggtataac
    25621 acaccagatg aggcgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact
    25681 ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac acaaatggcg
    25741 ctggtttcag aacgccaaga cgcgccgcga cctggctcac gcgctcaagg ccgagatcga
    25801 tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt
    25861 gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg
    25921 gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg
    25981 ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact
    26041 tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg
    26101 cgcctttctc gatcaggaat tcactgcgct ggaatcctgg cggcagtttt tcgcgaacgg
    26161 tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttcggttca gcgatgttga
    26221 gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg
    26281 agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct
    26341 gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca
    26401 gcgggcagtt atcttccagc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag
    26461 accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat
    26521 acagagtgcc tttcatcacc accagcgcat ctttttcgcc ggtttctttc tgcgcagatg
    26581 ccagacggtc tttatacttc ttggagtcac gaaacttcag cacatctttc ggctcaagct
    26641 cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg
    26701 tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc
    26761 ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct
    26821 tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg
    26881 tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc
    26941 aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg
    27001 gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat
    27061 tatttcgatg acgcccggca aaatagaaac cacaataatc ccgacgatca gcagcttaag
    27121 gttatcctga accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac
    27181 ccacaacagt gcgccgatca cgttataagc agcgaaatga cggtacgaca tgtggcccat
    27241 tcccgcaaca aacggggcga acgttctgac gatcggcaca aaacgggcga gaataatcgt
    27301 tttgccgcca tgtttctcat aaaactgatg ggttttatcg agataactgc gacggaaaat
    27361 tttcgaattg ggattactga ataacttttc accgaacaag cgcccgatag tgtaattgac
    27421 cgcgtcaccc acaatcgcgg cgatcagcat cagcaccacc atcatatgga cgttgagatc
    27481 gttggtttcc agtgatgcca acgctccggc aacaaacagc aatgaatcac ccggtaaaaa
    27541 cggcgttacc accagaccgg tttcacagaa caaaattaag aacaaaatgg cataaaccca
    27601 gacgccgtac tccgcgacca gctctgccag atgcacgtca atgtgcagga taaaatcgat
    27661 gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta
    27721 actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata
    27781 gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc gcgaccagat
    27841 acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact
    27901 ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat
    27961 tcctgaccat atgatgtaca aaggcattcg ctttgatatc aaccaccaca taaggaccgt
    28021 gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg
    28081 cacggaacga ggtgaaatca ttctcgccca gcaagcactg cgcagcccga tgcatccgtt
    28141 cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc
    28201 gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat
    28261 catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc
    28321 ccaacgtcca cgcagcgtct ttgcgctgcg cggtagtttc gaaatgcaca acctgcccgg
    28381 taccgtgtac ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca
    28441 cctgggagag cgccttttcc agcttctcct gcacactgcg gacttcattc tgccgttgcc
    28501 agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcggtt
    28561 gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc
    28621 catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagaactgga cttgctccgg
    28681 cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg aagcatcacc
    28741 tacctgagtt gggaattcgt tctcttcaga gagcacaata tcttcccctt gaacaaacgc
    28801 atcacgcgct tcttctgctg ccagtggacg cagagcttca aagttgacca tctgggcatg
    28861 accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagtcctt cgtcctgcag
    28921 gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg
    28981 cagtaacggc agcatgttga acgcgagctg acgcccgaag aaatcttctt cgtcaatcgg
    29041 aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt ttttgccctg
    29101 ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa
    29161 cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga tgacattccg
    29221 gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg
    29281 ttcaagagca aacaatccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc
    29341 aacccaggct gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca
    29401 gtcgaattca gcagcatcct gcacggtgat tgtcttacca ccaaagcgca attgttcgcc
    29461 tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag
    29521 cgtttcaagc agggcttcgc ccacagcgcc agttgtgccc aggacggcaa tgttccagcc
    29581 ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt atagcagaga
    29641 gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc
    29701 aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca
    29761 agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt
    29821 gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg
    29881 agcggtccat gcagagtaat gcgaccaaac tctggcgcag gcagtaatgt atccagcgca
    29941 acgtgctgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt agtaccgcgt
    30001 gctttacctt ccagggtata gcctgcaata tgcggcgtgc cgatatccac ttttgtcagc
    30061 aattccacat ttagttccgg ttcgccttcc cagacatcca gcactacgct taacttctgg
    30121 ccttcattca ggcaggtcag cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta
    30181 atcagaatcg ctccgggctt caggctacgg atcagttttt catccgccag atgcagcgtt
    30241 ttgtacggcc cgtctttgaa gagtggcgta tggaaagtca gaatatccgc gcgctggact
    30301 aactcatcca gcgagcggaa atcaccctca tccccacagt cggcgcgagg cggatcgcaa
    30361 agtaaggttt taatccctaa cgcttccagt cgtgcctgta aacgccgacc aacgttacca
    30421 acgcctacga tccccacggt acgctcgtgc agtgaaaatc catcgcgttc ggcaagcatc
    30481 agcagggagg agaaaacata ttccaccacc gcaatcgcat tacagccagg tgcagcggaa
    30541 aaaccaattc ccgcctgctt caaccatgct tcatcgacat ggtcggtccc cgcagtggca
    30601 gtgccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa
    30661 cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc
    30721 gcgatcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca
    30781 aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata
    30841 atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc
    30901 tatttccggc cctcctcctg ggcaaccacc aggtcaggga gataacctgc cgtctggcgc
    30961 gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt
    31021 gacctcactg acgcaacagc aaagagcaga actgtgggcg ggtatcaggc acgatattgg
    31081 tctgtcggga gattcaccgc tgctttcgcg tcactgccct gccgctgagc ataatctggc
    31141 gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg
    31201 ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt
    31261 tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt
    31321 gcaggaaggg aagatggtta ttccgcaacc gcagcagagc gaggcgaccg atcgtcctct
    31381 attaccggcg gagcacaatg cgctcaaaca gctggtgacc aaacttgcgg cggcaacggg
    31441 ggagcccagc aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga
    31501 gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ctgcaggcgc gtcagacgct
    31561 aagccagcaa aatacgccga cgctggaatc actacaaatg acgctaaaac aacctttaga
    31621 tgccagtgaa ctggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca
    31681 atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt
    31741 taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga
    31801 cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct
    31861 gatgctggtc tggctggttc gttaaccccg acgaaacgac agtatcgtga caataattcc
    31921 cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag
    31981 cattcccgca agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc
    32041 cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacac ccagcgcagg
    32101 aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga cccacgcccc
    32161 tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga caatcgccac
    32221 tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc
    32281 gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag
    32341 cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagac ctaaccctgg
    32401 tttccagata agtccgacaa cgctccacag agatggacgt tctcccgcca gggctggtac
    32461 tttgcgcact gtgccgttac aggcccacgc cagtaagggt aatgccattg tggtgagcgc
    32521 cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggagcac caacagcgag
    32581 ggcaccgtaa atcgccattc cattccatga catcactttg ccagagtgtt ttggccctac
    32641 gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa acccaagaat
    32701 caaacgcccg atgaccaaca gggcaaattt gaacggtgcg gagacaggca aaatcgccgc
    32761 cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg ccgaacgttt
    32821 tgcgccatat tgatcggcca gtcgcccggc atagccacgc gtcagcaccg tagccagaaa
    32881 ctgaatgccg acggcaatgc cgaccatggt attgccatag cccagatcat gatgaacaaa
    32941 caacgggata accggcaacg gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaagc
    33001 gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta cagcagtcat
    33061 gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca
    33121 gggttacagt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag
    33181 cggggagttt gcctggaact ggcggcgatt gtcaatgatg tgaaaaaggg aaccatcagg
    33241 ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg cgacgctggc
    33301 gcgtcttatc cgacctactc cggcctactg cgaattaatc tttcagcttg cgcattacca
    33361 gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat
    33421 cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga
    33481 tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct tcctgtacgc
    33541 cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat
    33601 cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtgc
    33661 cgtgggagtt caggtaatcg attggggtat caacgccatg catcgccatc tgcatacaac
    33721 gtactgcgcc ttcgccagag ggagcaacca tgtctgcgcc atcagacgtt gcgccgtagc
    33781 caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc tcttcaacca
    33841 ccaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcatcg taagtacggg
    33901 aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccattgcg tcgaattcgc
    33961 aagccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca
    34021 gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga
    34081 tggagtagtt aacgccatga attttaaacg gcgtggcgag gcaggcagaa acgccggatg
    34141 ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg ccgcgcattg
    34201 cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc agaacctgca atcaggccaa
    34261 cacgcggatt attctggtaa gcttccggag agaggcccgc atctgcgatt gcctgctcca
    34321 tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga
    34381 ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg gaatccttca
    34441 gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct
    34501 ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat
    34561 acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc
    34621 gaacaagtcc gatcagccaa ttaatagaga aatttgcgca gccttacaca catcgctaag
    34681 atcgagctac cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc
    34741 aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt
    34801 tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa ccaattagag
    34861 gcacgctttc ctgaacatcc acatcctctg tttgtggtag cagagagcgg cttcggcacc
    34921 ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc gcatccgcaa
    34981 gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat
    35041 ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg
    35101 cagtggccaa tgcccttgcc cggttgccat cgtttattac ttgatgaagg ccgcgtgacg
    35161 ctggatttat ggtttggcga tattaatgaa ctgatcagcc aactggacga ttctctaaat
    35221 caaaaagtag atgcctggtt tctggacggc tttgcgccag cgaaaaaccc ggatatgtgg
    35281 acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac gctggcgaca
    35341 tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgcaaaaa
    35401 cgtaagggct ttggacgcaa acgggaaatg ctttgtgggg tgatggaaca gacattacca
    35461 ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt
    35521 atcggcggtg gtattgccag cgcattgttg tcgctggcgc tattgcgtcg cggctggcaa
    35581 gtaacgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg
    35641 gcgttgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatggg
    35701 tttacttttg ctcgtcgact ttatgattca ttacccgtta aatttgatca tgactggtgc
    35761 ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca gatgttgtca
    35821 atggatttac ccgaagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg
    35881 ggcgttacga caaattgcag cggtattact tatccgcaag gtggctggct gtgtccggca
    35941 gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa
    36001 tatcagttac aggatttatc ccgtaaggat gactgttggt tgttgacgtt tgcaggagat
    36061 cagcaagcga cacacagtgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc
    36121 caaacatcgt ctctgccagt gtattcggtt gccggtcagg tcagccatat tccgacgaca
    36181 cccgaactgg caaaactgaa acaggtgctg tgctatgacg gttatctcac gccgcaaaat
    36241 ccggcgaatc agcaccattg tattggtgcc agttatcatc gcggcagcga agagacggcg
    36301 tacagtgaag acaatcagca gcagaatcgc cagcgattga ttgattgttt cccacacgca
    36361 caatgggcga agacggttga tgtcagtaaa aaagaggcgc gctgcggtgt gcgttgtgcc
    36421 acccgcgatc atctgccaat ggtaggtaat gttcccgatt atgaggcaac actcgtggaa
    36481 tatgcgtcgc tggcggagca gaaagataag gcggtaagcg cgccggtttt tgacgatctc
    36541 tttatgtttg ctgctttagg ttcgcgcggt ttgtgttctg ccccgctgtg tgccgagatt
    36601 ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta
    36661 aatccgaatc ggttatgggt gcggaaattg ttgaaaggta aagcggttaa ggccgggtaa
    36721 tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccgacct acgtgtgacg
    36781 tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac
    36841 ttactgctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc
    36901 acgcggcgac aattcaccag caccaatggc tttttccaga ctctggctaa cggtcgtatg
    36961 caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta atgtaaggtg
    37021 gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa ccataccgtc
    37081 gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac
    37141 gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg
    37201 cgtgtgataa taattttgta gtgctttaat gaaacgtgcc gggcgttccg gaatgccatt
    37261 tgttagataa tccatcacct gcgcatgtac tcgccgttga aacaccacgc ggtccggttc
    37321 gaagtcgcct tccagattgt cgcaactgac gttaaacggg tatcccgccg ccacgcagaa
    37381 caaccagtcg agcgcctgcg gcttcacttc aacatcttca aactggcttt gcgtttgggc
    37441 atcgcgaccg tccgggcaat accagtagcc gaaatcaacc tgctcgcggc gcgctttccc
    37501 ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccgt gggcaaagac
    37561 gattcggtta tacggtactt ccgcatcagc gggaagatag atcggttcgt cgtcgccttt
    37621 aatcagacgg gtattaaaat catcggcaaa gcagctatta aaaatttcaa tcaactgctc
    37681 atagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg
    37741 ctatcataaa gtaatttggc actcatcacc gccgagacga taacgatcat cgggcggatc
    37801 agtttttgtc ctttgctcaa caccagtctt gaacccatgc gcgcgccaag gaactgcccc
    37861 accagcatca caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagtaaa
    37921 ccgccgatgt ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt ggcgaggttg
    37981 aatccgcaca gcgtaacaaa ggccagcgcg taaaacgatc cggctgccgg accaaagaat
    38041 ccgtcataaa aaccgacgca gccaccggca attaacgcga agggcaaacc gtacatccgg
    38101 cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc
    38161 accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac caacagtgcg
    38221 ccgctcattg agccgacaaa ggtcatggca atattaagtt tctgatcgct taaactaacc
    38281 actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta
    38341 ttggttgcca gagcattagc gggagacatc cccgctgcca tcaatgccgg aatggtgagt
    38401 aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga
    38461 actcccagca acagcgggga aaccataaac aggctattaa acgtttccat tagatcacgt
    38521 gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct
    38581 ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc
    38641 ctggtggcgg taaaggttga tcttcacact ccagactatc ggcaggacaa cgtaatcgta
    38701 catgcatatg cgcacgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg
    38761 tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt
    38821 ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga
    38881 caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag agctgcgcgg
    38941 aggtccggcg tgttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc
    39001 tggcgtgacc gccgttgaaa cgcccaccag caggcattcc catatcgcca atcaacaccg
    39061 tacccatgcc cagattgctc acctgactac tcaggcgctg gataaacatc accaggtccg
    39121 ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg
    39181 gcaacgtgtc agcgccgacg atacaaccat tggaaaaact gcctatcgat tgtgcgctac
    39241 ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctgacg ctactggcaa
    39301 gcagggccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct
    39361 tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg atcgccagca
    39421 tcgcttctgc tatcggcacc gcgcggatcc cgacacaggg atcgtgacgg cctttggtga
    39481 tcatctcaac ttcttcgcca aagcggttaa tggtgcgccc cggcacggta atactggagg
    39541 tcggtttcag tgccatatga gcaatgattt gctgcccgct gctaattccg ccgagaatgc
    39601 cgcccgcatg gttactctgg aaaccgtctt tggtgatttc gtcgcggttc tggctgccac
    39661 gcagcgccac cacgtcaaaa ccatcaccaa tttccacgcc tttcaccgcg ttaatgctca
    39721 ttagcgcatg ggcgatgtcg gcatccaggc gatcaaagac cggctcacca agcccggcag
    39781 gaacgccact ggcaacaacg gtgactttcg cgccaataga gtcgccctct tttttcagcg
    39841 cgcgcatcag ttcatctaac gcgtcgattt tgtccgggtc cgggcagaag aatggatttt
    39901 gctcgacctg cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc
    39961 cgcgaatttc gatgccaaat ttctcagcga gatatttttt ggcaatcgcc cctgctgcaa
    40021 cgcgcatggc ggtttcacgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt
    40081 atttttgttc gtaggtgtaa tcggcatggc caggacggaa aacgtcctta atcgcactat
    40141 aatcctgaga acgctgatcg gtattttcga tcaacaaacc aatgctggtg ccggtagtaa
    40201 caccttcaaa aacaccggag agaattttga cctgatctgg ctcacgacgc tgggtggtat
    40261 agcgcgatgt cccagggcga cgacggtcga ggtcatgttg caggtccgct tccgtcagcg
    40321 gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga
    40381 aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt
    40441 tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttcacgggc cgccagcagc
    40501 tgcgctttgg tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac
    40561 ggaacatcgg gatattgttc cataagatgt accatgctgt tgccgacttc acaaatcagt
    40621 acgccatcat cagcaagata atctgccgcg tttccgagaa tgcgacgcgt cagtttcagg
    40681 ccgtcggtgc cagatgccag gcccagttcc ggctcgtggc ggtattcatt tggcaggtcg
    40741 gacatatctt ccgcatcgac atacggcggg ttagtgacaa tcaggtcgta ctgcactttc
    40801 ggcaaatcac ggaacagatc ggaacgaatc ggtatgacgt tgtggatcag accgtgttct
    40861 tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc atcgacttcc
    40921 gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct
    40981 aaaatatgct gcggttgctt gctgataagt ccggcaaatt tattgttgat caattcacca
    41041 atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatggcc gcagaaccac
    41101 gctttgttgg tcaggtaagc taccggaatg cgttcgttga cgcggcggat cacgcgctca
    41161 acaatacgat gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc cggaatatcc
    41221 agcggcaggt agagcgacgg caataccagc tgtacggctt catcccacgg gttatcggtg
    41281 ccgtgaccgt accagatatt tgccgcgctg aagcggctca ccgaccagcg caacatgtcc
    41341 tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc
    41401 agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca
    41461 ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgcgct acgagtcggg
    41521 taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg
    41581 aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca
    41641 ccattgtcca tcggccacag cgtaaaaaag tcagcgaagt gcccgtcaaa cggttgattc
    41701 aggagcaggt tgacgccagt cactatttct ctgacgaatt tcaaccgtta ttaaatgctg
    41761 atggcccggt gaagtatgta cgccctggtg tagaccattt tgaggcgaaa aagctgcgcc
    41821 gcggcgatta ttcgccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca
    41881 agcaggaact gggcgcgttg atcgccgcct gtcgccgcga acatgtgttt tgcgcctgcg
    41941 tgatgcatgg tcacgggaaa catatattga agcaacaaac accgctgtgg ctggcacaac
    42001 atccgcatgt catggcgttt caccaggcac caaaagagta tggtggtgat gccgcattgc
    42061 tggtgttgat tgaagtggaa gagtggctac cgccggagtt gccatgaata ttagggggcg
    42121 gatgcgtttt gccatttcgc cctgaatgct ggtatcgaat gccggatgcg gcgtaaacgc
    42181 ctgatccggc ttatcaaaat gtgcaaattc aatacgttgt gattcagttg taggcatgat
    42241 aagacgcgtc agcgtcgcat caggcatcaa cgcacaacta cagttcattc agtacatact
    42301 ggaaacggca tgtagaatca attggctcac ggctactggt attcacctgt accacaatgt
    42361 cgtgtagctt tctatccgtc aatggttgac gtgagggggt gactgtcacc tttgcattat
    42421 cacaaccata cgaggtaaag gttaataagt cagaaacagc acgacaatat gcccctctgg
    42481 aatcaaaaat actgccatcg cagagtttgc cgctttccat caggtaggtc gaaagcgtag
    42541 tcgtgattgt cgaaccatcg gattgcaatc gcagcgcttt attatggcta acataattat
    42601 cactccagga tatatcaaac gaagcgacgg gaattgtcgg gcaaatagaa tcatgaactg
    42661 ttgggcttgt atacgtacca aatccaaatg cctcagatac tgaataaaat gaggcaatcc
    42721 acggacaacc ataattatca gattctacag tgacgttaaa agtcatatat gcacagacaa
    42781 agacacgagt attgatacca gggcaatttg aggtgtccgt acgtctataa tcagtcaaaa
    42841 ccagtgtcgg tgccgggcca gatccgctcc aggatattaa cttgagggtg tcgccttttc
    42901 ttgcattgga gttgtattgt ccgtagctta cttctcccgg cgtcactacc gtgacataga
    42961 aatataaact gccggaagca gtaacgtttt tcatcgcata ataaatttta gtactggaaa
    43021 agcttgcact catgagttta tcaccggcat agcactttgg tgtttctaaa ctgagagcaa
    43081 gtaatatcag acttgtcaaa aatatcgtcc attttttcat tttaaaaata ctcaaaagtt
    43141 accgtcattg tggcggtgaa atcacccgat gtaacatttc gtccatttac ggtctgtaac
    43201 caggcattaa aattgacact gttatttccc tgatttaatg gaaatgaggc acctgtggta
    43261 ctgttaatag gtacagccgc ccctccggca gtttcaatgc caatcccaac acccgttgca
    43321 gaagatgacg aatcaatcga cagaaatcct ggtaagtcgg tatcttctgt tcccatcaag
    43381 gtcaccttga cattaaacgc cgtggtgctt ttgcaatctt tcaactgaaa aactaacggt
    43441 acgcgatctg actgaccacg ttgcattaaa tcgctggcag caattgtggg gaaatgaatt
    43501 tctgcaagta agttgc
//
LOCUS       DBFIFS010000013        42483 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.013, whole genome shotgun sequence.
ACCESSION   DBFIFS010000013 DBFIFS010000000
VERSION     DBFIFS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.013"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..177
                     /locus_tag="N7A07_000704"
                     /pseudo
     CDS             1..177
                     /locus_tag="N7A07_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(264..939)
                     /locus_tag="N7A07_000705"
                     /pseudo
     CDS             complement(264..939)
                     /locus_tag="N7A07_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1084..1692)
                     /gene="yiiX"
                     /locus_tag="N7A07_000706"
     CDS             complement(1084..1692)
                     /gene="yiiX"
                     /locus_tag="N7A07_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU2475432.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1752..2069)
                     /gene="metJ"
                     /locus_tag="N7A07_000707"
     CDS             complement(1752..2069)
                     /gene="metJ"
                     /locus_tag="N7A07_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU2475433.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2346..3506
                     /gene="metB"
                     /locus_tag="N7A07_000708"
     CDS             2346..3506
                     /gene="metB"
                     /locus_tag="N7A07_000708"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU2475434.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3509..5941
                     /gene="metL"
                     /locus_tag="N7A07_000709"
     CDS             3509..5941
                     /gene="metL"
                     /locus_tag="N7A07_000709"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU2475435.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6080..6214)
                     /locus_tag="N7A07_000710"
     CDS             complement(6080..6214)
                     /locus_tag="N7A07_000710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475436.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6290..7180
                     /gene="metF"
                     /locus_tag="N7A07_000711"
     CDS             6290..7180
                     /gene="metF"
                     /locus_tag="N7A07_000711"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU2475437.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7509..9689
                     /gene="katG"
                     /locus_tag="N7A07_000712"
     CDS             7509..9689
                     /gene="katG"
                     /locus_tag="N7A07_000712"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU2475438.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9783..10688
                     /gene="yijE"
                     /locus_tag="N7A07_000713"
     CDS             9783..10688
                     /gene="yijE"
                     /locus_tag="N7A07_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU2475439.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10715..11332)
                     /gene="yijF"
                     /locus_tag="N7A07_000714"
     CDS             complement(10715..11332)
                     /gene="yijF"
                     /locus_tag="N7A07_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU2475440.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11606..12709)
                     /gene="gldA"
                     /locus_tag="N7A07_000715"
     CDS             complement(11606..12709)
                     /gene="gldA"
                     /locus_tag="N7A07_000715"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU2475441.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12720..13382)
                     /gene="fsa"
                     /locus_tag="N7A07_000716"
     CDS             complement(12720..13382)
                     /gene="fsa"
                     /locus_tag="N7A07_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU2475442.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13394..15895)
                     /gene="ptsP"
                     /locus_tag="N7A07_000717"
     CDS             complement(13394..15895)
                     /gene="ptsP"
                     /locus_tag="N7A07_000717"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2475443.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEELRNALVNAASADAIASLLQHELEL"
     gene            16204..17283
                     /gene="frwC"
                     /locus_tag="N7A07_000718"
     CDS             16204..17283
                     /gene="frwC"
                     /locus_tag="N7A07_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU2475444.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17298..17618
                     /gene="frwB"
                     /locus_tag="N7A07_000719"
     CDS             17298..17618
                     /gene="frwB"
                     /locus_tag="N7A07_000719"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU2475445.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17669..19966
                     /gene="pflD"
                     /locus_tag="N7A07_000720"
     CDS             17669..19966
                     /gene="pflD"
                     /locus_tag="N7A07_000720"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU2475446.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19932..20810
                     /gene="pflC"
                     /locus_tag="N7A07_000721"
     CDS             19932..20810
                     /gene="pflC"
                     /locus_tag="N7A07_000721"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU2475447.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20812..21153
                     /gene="frwD"
                     /locus_tag="N7A07_000722"
     CDS             20812..21153
                     /gene="frwD"
                     /locus_tag="N7A07_000722"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU2475448.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21140..21991)
                     /gene="yijO"
                     /locus_tag="N7A07_000723"
     CDS             complement(21140..21991)
                     /gene="yijO"
                     /locus_tag="N7A07_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2475449.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22206..23939)
                     /gene="eptC"
                     /locus_tag="N7A07_000724"
     CDS             complement(22206..23939)
                     /gene="eptC"
                     /locus_tag="N7A07_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU2475450.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24122..26773)
                     /gene="ppc"
                     /locus_tag="N7A07_000725"
     CDS             complement(24122..26773)
                     /gene="ppc"
                     /locus_tag="N7A07_000725"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU2475451.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27125..28276)
                     /gene="argE"
                     /locus_tag="N7A07_000726"
     CDS             complement(27125..28276)
                     /gene="argE"
                     /locus_tag="N7A07_000726"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU2475452.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28430..29434
                     /gene="argC"
                     /locus_tag="N7A07_000727"
     CDS             28430..29434
                     /gene="argC"
                     /locus_tag="N7A07_000727"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU2475453.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29442..30218
                     /gene="argB"
                     /locus_tag="N7A07_000728"
     CDS             29442..30218
                     /gene="argB"
                     /locus_tag="N7A07_000728"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU2475454.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30279..31652
                     /gene="argH"
                     /locus_tag="N7A07_000729"
     CDS             30279..31652
                     /gene="argH"
                     /locus_tag="N7A07_000729"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU2475455.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31919..32836
                     /gene="oxyR"
                     /locus_tag="N7A07_000730"
     CDS             31919..32836
                     /gene="oxyR"
                     /locus_tag="N7A07_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU2475456.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32819..34219)
                     /gene="sthA"
                     /locus_tag="N7A07_000731"
     CDS             complement(32819..34219)
                     /gene="sthA"
                     /locus_tag="N7A07_000731"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU2475457.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34549..35715
                     /locus_tag="N7A07_000732"
     CDS             34549..35715
                     /locus_tag="N7A07_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HGU2475458.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            35758..37074
                     /locus_tag="N7A07_000733"
     CDS             35758..37074
                     /locus_tag="N7A07_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HGU2475459.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            37178..37828
                     /gene="fabR"
                     /locus_tag="N7A07_000734"
     CDS             37178..37828
                     /gene="fabR"
                     /locus_tag="N7A07_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU2475460.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            37828..38187
                     /gene="yijD"
                     /locus_tag="N7A07_000735"
     CDS             37828..38187
                     /gene="yijD"
                     /locus_tag="N7A07_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU2475461.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38227..39327)
                     /gene="trmA"
                     /locus_tag="N7A07_000736"
     CDS             complement(38227..39327)
                     /gene="trmA"
                     /locus_tag="N7A07_000736"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU2475462.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECSVLLTAK"
     regulatory      39441..39631
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            39696..41540
                     /gene="btuB"
                     /locus_tag="N7A07_000737"
     CDS             39696..41540
                     /gene="btuB"
                     /locus_tag="N7A07_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU2475463.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41485..42342
                     /gene="murI"
                     /locus_tag="N7A07_000738"
     CDS             41485..42342
                     /gene="murI"
                     /locus_tag="N7A07_000738"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU2475464.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9661 a        10571 c        11768 g        10483 t
ORIGIN      
        1 tattttaata tataaagtaa atataattaa tgctagttca aaaaagatgt atttttttat
       61 gaaagcccaa catcaaccca caatgacata atggggggcg ggggaatgtc atatgcctat
      121 gaatatgatg taaaatataa tatatgtcat ttgagaaaaa agagcagaat gaggtaaaaa
      181 tctttggctg tgtattcagt ctggtaatca tccaaggaaa gctcatcaga agggtgatga
      241 ctccaattag tagaacatat attttcgata aaagcaccga tgactttttc atgaagttct
      301 acagaactcg agaaacagat ggttctgcgg gccagacgtt tgattcgggt tctcagggtc
      361 agattgttac gctcaatgtg ctgagtaaaa atcttaccag tcagatgctt ctcctttggt
      421 aactctctag cgtaactacc ccattcgtca gtggtcaaca tgccgatggt aaaagggtaa
      481 gcagcgccag caattcacga caggtttcat cagtccgggg gccaaaagtg taggccagaa
      541 caccaccagt tttcgtgttg tacacgtacc agagccagtg ctgtcgcgct ttactgccga
      601 caaagctcca ttgctgacta agtttgcaga tgagtgccac atcagcatga gcgaccgggg
      661 atgacgttat tcttcgtggc gagagttttt taaagttcgg atgactgtat taatgccgat
      721 tttcagcgta cgggcagtat agcgaacccc ggcaccgttg aaagccattt cagcgatttg
      781 gtctttaact cccggtttac ggggctcgta acagtaactg agctgaaata cccggtgaca
      841 gtcacggccg cggaatcgat catgtctttt ggggttctgc ccgcgacggt aaacctgaac
      901 ggactggcaa cggggacaat gaaggttaac tgtgaccatg aaacatcctt aaaagcgagt
      961 attatatcct taattcaact aattggaggc atcaccgttt accggagcga cagcttgcgg
     1021 aaaggtcgtg caactacaaa gcaacaaaca gactacggcg ggaattattt ttttcatcat
     1081 ccattaccac gaaaacagcg gccattcttt ggcaaccgtg gtgagttgtg gcgcatcgaa
     1141 aacggcttgt ggtgagacca ccgtttcctc aagcggaata tttttgccgt agcgttcttt
     1201 gagcttcgcc tggaccagcg ggttgctgag atcaaactct ttcaacttct gctgttcgcc
     1261 cacacgcatt cccagcgcat tctgataaac cttccacacc acttccgaac agtactggcg
     1321 atcgtcgctc catgagaagc tgaaatcgta cggtttgccg agataacgtt tcgccgtttg
     1381 cgccagtttt tgctgttgtt caacactcag tccgccttca acgcggcgaa caacgtattt
     1441 gcccttttca ccatgggcaa tccactgctt gagcggggtg tatttcaccg ggccgaccgc
     1501 ttcaaaaacg tagggctttt tgttacgcat caccagcata ccggtgtggc tgtaatcgga
     1561 atgggtcgcc agttggatcg ctttactttg cgatgagcga gagatttgaa agatgatgtc
     1621 gccagtttgc ggctgccagg caaaggcagg cacagaaacc agcaggctga ggatcagtag
     1681 cctgattttc atcgttaaac gtccttgtat gaaaaaagcg gctggttaac tccgcgccgc
     1741 tcttttccgc tttagtattc ccacgtctcc gggttaatcc ccatctcacg catgatctct
     1801 tttgccgctt ccgggatttc gtcgctgcgc tctttacgca gatcggcatc atccggcaaa
     1861 ggttgcccgg taaaggcatg cagaaacgct tcgcacagca gctcgctgtt ggtagcgtga
     1921 cgcaggttgt tcacctgacg acgcgtgcgt tcatcggtga ggatttttaa caccttaaga
     1981 ggaatggaaa ccgtaatctt tttgacttgt tcactcttct tgccgtgctc agcgtatggg
     2041 ctgatatatt cgccgctcca ttcagccatg agatacttaa tcctcttcgt caataaattg
     2101 agaccagacc acacaattta tgtgggtact gaccgtaaac ccgcatagtt taccgtacag
     2161 gcgttaccgt gacatcgtgt aatgcacctg tcggcgtgat aatgcatata attttaacgg
     2221 ctatttggga tttgctcaat ctatacgcaa agaagtttag atgtccagat gtattgacgt
     2281 ccattaacac agtgtttact ctggtgcctg acatttcacc gaccaagccc agggaacttc
     2341 atcacatgac gcgtaaacag gccaccatcg cagtgcgtag cgggttaaat gacgacgaac
     2401 agtatggttg cgttgtccct cccatccatc tttccagcac ctataacttt accggattta
     2461 atgaaccgcg cgcgcatgat tactcgcgtc gcggcaaccc aacgcgcgat gtggttcagc
     2521 gtgcgctggc agaactggaa ggcggtgctg gtgcagtact tactaatacc ggcatgtccg
     2581 cgattcatct ggtaacgacc gtctttttga aacctggcga tctgctggtt gcgccgcacg
     2641 actgctacgg cggtagctat cgcctgttcg acagcctggc gaaacgcggt tgctatcgcg
     2701 tgttgtttgt tgatcaaggc gatgaacagg cattacgggc agcgctggca gaaaacccca
     2761 aactggtact ggtagaaagc ccaagtaatc cattgttacg cgtcgtggat attgcgaaaa
     2821 tctgccatct ggcaagggaa gtcggggcgg tgagcgtggt ggataacacc ttcttaagcc
     2881 cggcattaca aaatccgctg gcattaggtg ccgatctggt gttgcattca tgtacgaaat
     2941 atctgaacgg tcactctgac gtggtggctg gcgtggtgat tgctaaagac ccggacgttg
     3001 tcactgaact tgcctggtgg gcaaacaata ttggcgtgac gggcggcgcg tttgacagct
     3061 atctgctgct acgcgggttg cgaacgctgg tgccgcgtat ggagctggcg cagcgcaacg
     3121 cgaaggcgat tgtgaaatat ctgcaaaccc agccgttggt gaaaaaactg tatcacccgt
     3181 cgctgccgga aaatcagggg catgaaattg ccgcgcgtca gcaaaaaggc tttggcgcaa
     3241 tgttgagttt tgaactggat ggcgatgagc agacgctgcg ccgtttcctg ggcgggctgt
     3301 cgttatttac gctggcggaa tcattagggg gagtggaaag tttaatctct cacgccgcaa
     3361 ccatgacaca tgcaggcatg gcaccagaag cgcgtgctgc cgccgggatc tccgagacgc
     3421 tgctgcgtat ctccaccggt attgaagatg gcgaagattt aattgccgac ctggaaaatg
     3481 gcttccgggc tgcaaacaag gggtaaaaat gagtgtgatt gcgcaggcag gggcgaaagg
     3541 tcgtcagctg cataaatttg gtggcagtag tctggctgat gtgaagtgtt atttgcgtgt
     3601 cgcgggcatt atggcggagt actctcagcc tgacgatatg atggtggttt ccgccgctgg
     3661 tagcaccact aaccagttga ttaactggtt gaaactaagc cagaccgatc gtctctctgc
     3721 gcatcaggtt caacaaacgc tgcgtcgcta tcagtgcgat ctgattagcg gtctgctacc
     3781 cgccgaagaa gccgatagcc tcattagcgc ttttgtcagc gatcttgagc gcctggcggc
     3841 gctgctcgac agcggtatta acgacgcagt gtatgcggaa gtggtgggcc acggcgaagt
     3901 gtggtcggcg cgcctgatgt ctgcggtgct taatcaacaa gggctgccag cggcctggct
     3961 tgatgcccgc gagtttttac gcgctgaacg cgctgcgcaa ccgcaggttg atgaagggct
     4021 ttcttacccg ttgctgcaac agctactggt acaacatccg ggtaaacgtc tggtggtgac
     4081 cgggtttatc agccgtaaca acgccggtga aacggtgctg ctggggcgta acggttccga
     4141 ctattccgcg acacaaatcg gtgcgctggc gggtgtttct cgtgtaacca tctggagcga
     4201 cgtcgccggg gtatacagtg ccgatccgcg taaagtgaaa gatgcctgtc tgctgccatt
     4261 gctgcgtctg gatgaagcca gcgaactggc gcgtctggcg gctcccgttc ttcacgcccg
     4321 tactttacag ccagtatccg gtagcgaaat cgacctgcag ctgcgctgta gctacacgcc
     4381 ggatcaaggc tccacgcgca ttgaacgcgt gctggcctcc ggtactggtg cgcgtattgt
     4441 caccagccac gatgatgtct gcttgattga gtttcaggtg cccaccagcc aggatttcaa
     4501 actggcgcat aaagagatcg accagatcct gaaacgtgct caggtgcgcc cactggcggt
     4561 tggcgtgcat aacgatcgcc agttgctgca attttgctac acctcagaag tggccgacag
     4621 tgcgctgaaa atcctcgacg aagcgggatt acctggcgaa ctgcgcctgc gtcaggggct
     4681 ggcgctggtg gcgatggtcg gggcgggtgt cacccgtaac ccgctgcatt gccaccgctt
     4741 ctggcagcaa ctgaaaggcc agccggtcga gtttacctgg cagtccgatg acggcatcag
     4801 cctggtggca gtactgcgca ccggcccgac cgaaagcctg attcagggcc tgcatcagtc
     4861 cgtcttccgc gcagaaaaac gcatcggcct ggtattgttc ggcaaaggca atatcggttc
     4921 ccgctggctg gaactgttcg cccgcgagca gagcacgctt tcggcgcgta ccggctttga
     4981 atttgtgctg gcaggcgtgg tggacagccg ccgcagtctg ttgagttatg acgggctgga
     5041 cgccagccgc gcgttagcct tcttcaacga tgaagcggtt gagcaggatg aagagtcgtt
     5101 gttcctgtgg atgcgcgccc atccgtatga tgatttagtg gtgctggacg ttaccgccag
     5161 ccagcagctg gctgaccagt atcttgattt cgccagccac ggtttccacg tcatcagtgc
     5221 caacaaactg gcgggagcaa gcgacagcaa taaatatcgc cagatccacg acgccttcga
     5281 aaaaaccggg cgtcactggc tatacaacgc caccgtcggt gcgggcttgc cgatcaacca
     5341 taccgtgcgc gatctgatcg acagcggcga tactattttg tcgatcagtg ggatcttctc
     5401 cggcacgctc tcctggctgt tcctgcaatt cgacggtagc gtgccgttta ccgagctggt
     5461 ggatcaggcg tggcagcagg gcttaaccga gcctgatccg cgtgacgacc tctccggcaa
     5521 agacgtaatg cgcaagctgg tgattctggc gcgtgaagcg ggttacaaca ttgaaccgga
     5581 ccaggtacgt gtggaatcgc tggtgcctgc tcattgcgaa ggcggcagca tcgaccattt
     5641 ctttgaaaat ggcgatgaac ttaacgagca gatggtgcaa cggctggaag cggcccgcga
     5701 aatggggctg gtgctgcgct acgtggcgcg tttcgatgcc aacggcaaag cgcgtgtggg
     5761 cgtggaggcg gtgcgtgaag accatccgct ggcatcactg ctaccgtgcg ataacgtctt
     5821 tgccatcgaa agccgctggt atcgcgacaa cccgctggtg atccgtgggc ctggcgcggg
     5881 gcgcgacgtc accgccgggg cgattcagtc ggatatcaac cggctggcac agttgttgta
     5941 atttcagaaa tttaataatg cccggtactc atgttttcgg gtttatggtt tctaatgaaa
     6001 tatattgaat tatcatagga ttaggccgga tgaagcgttt acgacgaatc cggcaagaag
     6061 caataagtac atggttagtt tatatttgca gtccggtttg ctttgcatac cggattttct
     6121 ttttcttacc atcctgaagt tttttcatct tccctgattt ttcctcacca tcattggtca
     6181 tttttcggtt gacgcccttc ggcttttcct tcatctttac atctggacgt ctaaacggat
     6241 ggatgtgcac aacacaacat ataactacaa gcgattgatg aggtaaggta tgaacttttt
     6301 tcacgccagc cagcgggatg ccctgaatca gagcctggca gaagtccagg ggcagattaa
     6361 cgtttcgttc gagtttttcc cgccgcgtac cagtgaaatg gagcagaccc tgtggaactc
     6421 catcgatcgc cttagcagcc tgaaaccgaa gtttgtatcg gtgacctatg gcgcgaactc
     6481 cggcgagcgc gaccgtacgc acagcattat taaaggcatt aaagatcgca ctggtctgga
     6541 agcggcaccg catcttactt gcattgatgc gacgcccgac gagttgcgca ccattgcacg
     6601 cgactactgg aataacggta ttcgtcatat cgtggcgctg cgtggcgatc tgccgccggg
     6661 aagtggtaag ccagaaatgt atgcttctga cctggtgacg ctattaaaag aagtggcaga
     6721 tttcgatatc tccgtggcgg cgtatccgga agttcacccg gaagcaaaaa gcgcccaggc
     6781 ggatttgctt aacctgaaac gcaaagtcga tgcaggagcc aaccgcgcga ttactcagtt
     6841 cttctttgat gtcgaaagct acctgcgttt tcgtgaccgc tgtgtgtcgg cgggcattga
     6901 tgtggaaatc attccgggca tattgccggt atctaacttt aaacaggcga agaaatttgc
     6961 cgacatgacc aacgtgcgta ttccggcgtg gatggcgcaa atgtttgacg ggctggatga
     7021 cgatgccgaa acccgcaagc tggtgggcgc gaatattgcc atggatatgg tgaagatttt
     7081 aagccgtgaa ggagtgaaag atttccactt ctatacgctt aaccgcgctg aaatgagtta
     7141 cgcgatttgc catacgctgg gggttcgacc tggtttataa atagtgtggc ttttatgaaa
     7201 atcacacagt gatcacaaat tttaaacaga gcacaaaatg ctgcctcgaa atgagggcgg
     7261 gaaaataagg ttatcagcct tgttttctcc ctcattactt gaaggatatg aagctaaaac
     7321 ccttttttat aaagcatttg tccgaattcg gacataatca aaaaagctta attacgatca
     7381 atttgatcta catctcttta accaacaata tgtaagatct caactatcgc atccgtggat
     7441 taattcaatt ataacttctc tctaacgctg tgtatcgtaa cggtaacact gtagagggga
     7501 gcacattgat gagcacgtca gacgatatcc ataacacgac agccactggc aaatgcccgt
     7561 tccatcaggg cggtcacgac cagagcgcgg gggcgggcac aaccactcgc gactggtggc
     7621 caaatcaact ccgtgttgac ctgttaaacc aacattctaa tcgttctaac ccgctgggtg
     7681 aggactttga ctaccgcaaa gaattcagca aattagatta ctacggcctg aaaaaagatc
     7741 tgaaagccct gctgacagaa tctcagccgt ggtggccagc cgactggggc agctacgccg
     7801 gtctgtttat tcgtatggcc tggcacggcg cggggactta ccgatcaatc gatggacgcg
     7861 gtggcgcggg tcgtggtcag caacgttttg caccgctgaa ctcctggccg gataacgtaa
     7921 gcctcgataa agcgcgtcgc ttgttgtggc caatcaaaca gaaatatggt cagaaaatct
     7981 cctgggccga cctgtttatc ctcgcgggta acgtggcgct ggaaaactcc ggcttccgta
     8041 ccttcggttt tggtgccggt cgtgaagacg tctgggaacc ggatctggat gttaactggg
     8101 gtgatgaaaa agcctggctg actcaccgtc atccggaagc gctggcgaaa gcaccgctgg
     8161 gtgcaaccga gatgggtctg atttacgtta acccggaagg cccggatcac agcggcgaac
     8221 cgctttctgc ggcagcagct atccgcgcga ccttcggcaa catgggtatg aacgacgaag
     8281 aaaccgtggc gctgattgcg ggtggtcata cgctgggtaa aacccacggt gctggtccga
     8341 catcaaatgt aggtcctgat ccagaagctg cgccgattga agaacaaggt ttaggttggg
     8401 cgagcactta cggcagcggc gttggcgcag atgccattac ctctggtctg gaagtggtct
     8461 ggacccagac gccgacccag tggagcaact atttcttcga gaacctgttc aagtatgagt
     8521 gggtacagac ccgcagtccg gctggcgcaa tccagttcga agcggtagac gcaccggaaa
     8581 ttatcccgga tccgttcgat ccgtcgaaga aacgtaaacc gacgatgctg gtgaccgacc
     8641 tgacgctgcg ttttgatcct gagttcgaga agatctctcg tcgtttcctc aacgatccgc
     8701 aggcgttcaa cgaagccttt gcccgtgcct ggttcaaact gacgcacagg gatatggggc
     8761 cgaaatctcg ttacatcggg ccggaagtac cgaaagaaga tctgatctgg caagatccgc
     8821 tgccgcagcc gatctacaac ccgaccgagc aggacattat cgatctgaaa ttcgcgattg
     8881 cggattctgg tctgtctgtt agtgagctgg tatcggtggc ctgggcttct gcttctacct
     8941 tccgtggtgg cgacaaacgc ggtggtgcca acggtgcgcg tctggcatta atgccgcagc
     9001 gcgactggga tgtgaacgcc gcagccgttc gtgctctgcc tgttctggag aaaattcaga
     9061 aagagtctgg taaagcctcg ctggcggata tcatcgtgct ggcaggtgtg gttggcgttg
     9121 agaaagccgc aagcgccgct ggtttgagca ttcatgtacc gtttgcgccg ggtcgcgttg
     9181 atgcgcgtca ggatcagact gacattgaga tgttcgaact gctggagcca attgctgacg
     9241 gtttccgtaa ctatcgcgct cgtctggatg tttccaccac cgagtcactg ctgattgata
     9301 aagcccagca actgaccctg accgcgccgg aaatgaccgc gctggtgggc gggatgcgtg
     9361 tactgggtgc caacttcgat ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat
     9421 tgagcaatga cttcttcgtg aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg
     9481 aatcgaaaga gctgttcgaa ggccgtgacc gcgaaaccgg cgaagtgaaa tacactgcca
     9541 gccgtgcgga tctggtattt ggttctaact ctgtcctgcg tgcggtggct gaagtttacg
     9601 ccagcagcga tgctcacgag aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga
     9661 tgaacctcga ccgtttcgac ctgctgtaat ctgaccttct tcagcgactg cctttcaggc
     9721 agtcgctgaa gtttctttac cggcgtatag tgtccacagg aaaactacac actggatctc
     9781 tcatgtctgc cgcaggaaag agcaacccac tggcaatcag tggcctggtt gtgctcacac
     9841 ttatctggag ttatagctgg attttcatga agcaagtcac cagctacatc ggtgccttcg
     9901 actttaccgc cttacgctgc attttcggcg ctctcgtttt attcatcgtc cttttattac
     9961 gtggtcgcgg aatgcgcccg acaccgttta aatacacctt agccattgcc ctgttacaaa
    10021 cctgcgggat ggttggcctg gcgcagtggg cgctggtcag cggcggagcg gggaaggtgg
    10081 cgatcctgag ctataccatg ccgttctggg tggtgatttt cgccgcggtg tttctcggtg
    10141 aacgcctgcg acgtgggcaa tatttcgcga ttctgattgc cgctttcggc ttatttttgg
    10201 tgttgcagcc gtggcaactc gatttctctt cgatgaaaag tgccatgctg gcaatcctct
    10261 ccggtgttag ttggggggcg agcgcgattg ttgctaaacg tctgtatgcc cgtcatccgc
    10321 gcgtggattt attgtcgtta acatcctggc agatgctcta tgcggcgctg gtgatgagtg
    10381 tggtcgcttt actggtgccg caacgtgaaa ttgactggca gcccactgtg ttctgggctc
    10441 tggcctacag tgcgattctg gcgacggcgt tggcgtggag cttatggctg tttgtgctga
    10501 aaaacttgcc tgccagtatt gccagcttaa gcacactggc cgttcccgtt tgcggcgtac
    10561 tcttttcctg gtggctgctc ggtgagaatc cgggggccgt tgaaggtagc ggtattgtgc
    10621 tgattgtgct ggcactggcg ctggtgagcc gtaagaaaaa agaagccgtt agtgtaaaaa
    10681 ggatctgaat tttttcttca tgtggggcga tcgcttattt aacaaaataa cgataatgcc
    10741 ccaccatgcg ccagctaaac agcacatctt cttcctgcgc acctgcgcca atgttatgta
    10801 tcaccagcgg cgtaccgtcg cgggcgaagc catctgaaac caccccaata tgtgccagcc
    10861 cgttatctaa tcgccaggag acaatatcgc ccgcttgata gtcactgggg ttcttgctga
    10921 tggggcgtgt tttatcgtgg cgggtaaacc aggtttccag attaggcacc cgacggtgat
    10981 cgatgttgct gtccgggcgc tttaactgcc atttttgcgg gtactcggca aaattcttcg
    11041 ccatatcttc gtgcaccagt ttctgcaaat cgaccttctg gctgcgcaat gcacggatca
    11101 ccacatcgga acataccccg cgttcttgcg ggacatcacc gccaggatag gtaagctgca
    11161 catacgccgg atcgtaaaat agcgtgctgc cgatttgctg tctggcaccg tctgcgatgg
    11221 caaggttggt attggcctgg atttgtacca cggttggtgg aatggcggga gattttaagg
    11281 agtagctggt aaatgccgtt agcaggctga ccagcgccag tgaagctttc atctgtttcg
    11341 tccgtgaaaa taagagtcac agattcgacc ttccgggcta acgattgttc tgtagagaaa
    11401 atgtccagaa gcggataaaa aattcctgaa caacgtggga gtttcagatt actggcacag
    11461 agtgccggat gcggcgtgaa cgtcttatct ggcctaaaaa gcacgcaaat tcaatatatt
    11521 gcagagattg cgtaggcctg ataagcgtag cgcatcaggc aattttgcgt tcaaactccc
    11581 ggacaagccg ggagtttgga gtaggttatt cccactcttg caggaaacgc tgaccgtact
    11641 ggtcggctac cagcagagcg gcgtaaacct gatctggcgt cgcgccgcca ggcatgttgt
    11701 ggatggtttc accttctgca catgccgctt ctgccacaat tcgcattttc gccgggacat
    11761 cttctttaat atccagttga gcgagagtta ttggcaaacc taccgcatgg ctaagcgcag
    11821 ctacggtttc gatttcctca accggcgagt tttccagtac cagctgcgtc agtgtaccaa
    11881 atgccacttt ttcaccgtga taatagtgat gggcatcagg aatggcagtc aggccattat
    11941 gcaccgcgtg cgctgccgcc agaccaccgc tttcaaagcc aacaccgctt aaataagtgt
    12001 tcgcttcaat cacgcgttcc agtgccggag tcactacatg ctgttcggcg gcgagcatcg
    12061 ctttttcgcc ttcttccagc aaggtgttgt agcacagttc agccagcgcc agcgccgcct
    12121 gagtgcactt gccgcccgcc atggtggtcg caccgctacg agagcaggca cgcgcttcaa
    12181 accaggtcgc cagcgcatcg ccgatacccg ccgctaacag acgcgcaggt gcgcccgcga
    12241 cgattttagt atcgacaatc accatattcg ggttatttgg cagcagcaga tagcggtcaa
    12301 attcaccttc gtcagtgtag ataacggaaa gagcgctaca aggtgcgtcg gttgaagcaa
    12361 tggtcggggc aatggcgacc ggaacaccca tgaaatgtgc cagtgctttg gctgtatcga
    12421 gggtttttcc gccaccgata ccgagaattg cgccacactg cgcagtctcc gcgatgccac
    12481 gcagacggtc gatctcattt tgcgaacatt caccgccaaa cggcgcaatc tctactacca
    12541 gtccagcatc tttaaagctt ttctcgacag tggattgagc aaaacctaaa acaaatttgt
    12601 cacccaccac taaccagcgt tctgccagcg gcttcaggta ttcgcccaga cgattaatca
    12661 catcagcgcc ctggatgtat ttacccggtg attgaataat gcggtccata attgctcctt
    12721 tagagatgag tagtgccaaa tgcggcattc cagtcgtgtt cgaacttctc tatagctgac
    12781 tctaccgcag gggtgttgag catttgttgc gctacatcta agggcagggt gatggattca
    12841 catcctgcca gtaaacagtc cagcgcctga cgcggcgttt taaagctggc cgccagcacc
    12901 atgctttctg gtgcgtgcat ttctaacagc gcttgcagct cctgaaccgt acgaatgccg
    12961 tccccgccct gggcatccac gcggttaaca tacggagcaa cgtattttgc ccctgccagt
    13021 gcggctaata acccttgtgc ggcgctatat acagcagtgc caagtgtagt aataccctct
    13081 tttttcagta ttttaattgc tgccagacct tcggaagtca ccgggatttt caccacaatg
    13141 cccggaatgg catcgcgcag acgcttcgct tcttccacca tcccctgcgc gtcgcggctc
    13201 atggtctgag caaacagaat gccctcatca ccaatcgctt tttgcagacg cggcagcact
    13261 tcccatatgg actccttgct ggcagcgata atgctcgggt tagttgtcac cccggcaatc
    13321 gggaatatgt gtgccagacg ttcgacttct gcgacgttag cggtgtccag atacagttcc
    13381 atgatgtttc cttttacagt tccagttcat gttgcagcag gctggcgata gcgtcggcag
    13441 aggcggcgtt aaccagcgcg ttacgtaatt cttcgtgcat gatgcggcga gcgaggcgcg
    13501 agaaaatgcg catatgctga tcgcccgcag cgtgtttgtt cagggttaac ataatgatga
    13561 attgcgcttc atcatcgccc cagcgcaccg gcgcttgcag acgcgctacg ctgatggtgg
    13621 attgctcaat gtgttctgat ttgctgtggg gaatggcaaa actaaagccc agaccggtag
    13681 agaaaacggc ctcgcgcgcc cacaagtcgg cttccagttt acgcggatag cggcagcggc
    13741 ccgccagcag caggttatcg gtcatgcctt tgagcacttc ttctttgctg cgccagtcgc
    13801 tttccagtgt gatgcactcg gcggtgacca gcggtgcgtc ttgttgggtc atgcggaatt
    13861 gcgccagcag gtgttccact tccagcgaag tacggcaggc cattgcctgg ttgagcaact
    13921 tgcggcactc acggctatca agttgcgcca tccgcgcttt cgccgccgga attgatggtg
    13981 cgctcatgct gagttcatcc agccctaagc cgaccagcaa cggcagcacg gaacctttcg
    14041 ctcccagctc accgcacaga ccaatccatt tgccctggcg atgcaccgct tgcacggcgt
    14101 aatcgagcgc ccgcaagaat gccggattca ggctgttgta gtgacgagta accttagcgt
    14161 tatcgcgatc cactgccagc agatattgcg tcaggtcatt actaccaata ctaaagaaat
    14221 caatctcttc gcagcattga tcgatgatga acatcaccga cggcacttcc agcatgatgc
    14281 cgagctggat cttctcatca aacggaatgt gttcgttacg cagttgctgt ttggcttccg
    14341 ccagtttttc tttcacccat aagatctctt ccattgagga gatcatcggg atcatgattt
    14401 tcaggctgcc gtgagcagag gcacggagga tcgaccgtag ttgtgtggtg aacagcgagg
    14461 cgtattcttc ataaatacgc accgcgcgat aaccgaggaa cgggttagct tctgccggaa
    14521 tgttcagata atcaacgggt ttgtcaccgc caatatccat agtgcgcaca ataatgctgc
    14581 gtccgttggc ggactccagc gcttgacaaa aaatgttgta caactcgctt tcgcccggtg
    14641 cgctggtgcg atccatatag agcatttcag tgcggaacaa accgacgcct tccgcaccat
    14701 tgccgaatgc ggcctgcgct tccacggagt gagcgatgtt agcggcaatt tcaatgcgga
    14761 taccgtcagc ggtacgggct tgttgggtca gccagacacg ctgttgctca cgcagggcgt
    14821 cctgtacgcg ggcttcttgc tgataataac gagctacggc ttcccctggc tcaaccacaa
    14881 tcgccccggc gttgccgtcg atataaatcg tttgatgctg ccacggagta agggcatcaa
    14941 tatctacacc aaccagtgtc ggaatgttga acgaacgggc aagaattacg gtatgtgagg
    15001 tggtgccgcc acttttcagt aacagacctt tgaggtgatt tttatccagt tcgaggaact
    15061 ggctgggggt tagttcatca gccatacaaa tggcgggctg cgtcagtttg cccggtgccg
    15121 ggaagcgttg ctcaccgtag atttgctgaa gcagctggaa gcagacatcg cgtacgtcca
    15181 gggcacgttc ttgcaggtag ctgctgctgg aacgggaaaa ctcttcacaa aagtgattcg
    15241 cactggcgac aatcgcttcg gcgcagctta atccggcgct gacgcctgcc agtaaatgtt
    15301 cgtgcaggga agtatcgcca gccagggatc ggtgcgcttc cagaatcgcg ctggtggcac
    15361 cgtcgctatc cagcagacga aactcaatgt ttttcagtac cagcgtcagg ccgttttcca
    15421 gtgcggattg ctcggcgtca acgtctttgg ctgcgggaag attacccagc gcattgagat
    15481 ctaaagaaga gatcggcgtc agaatgccgc ctgcgctacc gctgcacacg gtgcgggcgc
    15541 ggataatttg cggattcaga ttggtcagtg aaaccggcag tggttccagt tcgtcagatt
    15601 taacttccgc cagcggcgca tcgcagtggg ggaattcatc gcgcagccat tggcttaaac
    15661 gctggtgcgc ttcctgttcg tcggccccgg aaatcagtaa ctggcagtta tcgcccgcca
    15721 gcgtgtcggt gccaatcagc gcaagggcgc ttttggcgtt gcccttgcgg tcagtgcgca
    15781 ggttatgcca ctcaatttgt gatgaaaaag tattacacag cgtttcaacg tggcttgccg
    15841 gacgtgcatg tacgccgtta ggtagctcac aaataaattc cacaatcagg gccattgcct
    15901 ctctcccggt acggttttct gtttacagga taagagcgca cggcaacggc ctgccatgtg
    15961 acaaatctgc caaaagctgg acaaatgtaa tgtaaccgtc aatttgcgac gcgtctcaca
    16021 agacgccgtt ttgcggcatg cttccggatt atcgcaagtt atgaggcgga tcgcattttt
    16081 gtactgatat tacaaaagtc cagtaaatgg cctttttatc cactgtttgc tccgcctgcg
    16141 attgcctatt gtttcctcca tcaaaaatat ggacatgggc tttcgcaagc ccgggagcag
    16201 cttatgaatg agttggtgca gatcctgaaa aatacccgtc agcatttaat gacgggcgtt
    16261 tcacacatga ttcccttcgt ggtatcgggc ggtattttgc tggcggtttc cgtcatgttg
    16321 tatggcaaag gcgcagtgcc ggatgccgta gccgatccga atctgaaaaa actgtttgat
    16381 atcggcgttg caggtttgac gctgatggtg cctttcctcg ccgcttacat cggttactcc
    16441 attgcagagc gttctgcgct ggctccgtgc gctatcggtg cctgggttgg taacagcttt
    16501 ggtgcgggct tctttggtgc actgatcgcc gggattatcg gcggcatcgt ggtgcattac
    16561 ctgaagaaaa ttccggtgca taaagttctg cgctcggtga tgcccatctt catcattccg
    16621 atcgtcggca cactgattac cgcaggcatc atgatgtggg gtctgggcga gcctgtaggg
    16681 gcgttgacca acagcctgac tcagtggctt caggggatgc agcagggcag cattgttatg
    16741 ctggcggtga tcatgggtct gatgctggcg ttcgatatgg gcggtccggt taacaaagtg
    16801 gcctatgcct tcatgctgat ttgcgttgct cagggtgttt ataccgtggt ggccattgct
    16861 gccgtgggta tttgtatccc gccgctgggg atggggctgg cgacgctgat tggtcgtaaa
    16921 aatttctccg cagaagagcg cgaaaccggt aaagcggcgc tggtgatggg gtgcgttggg
    16981 gttactgaag gggcgattcc tttcgccgca gccgatccgt tacgtgtaat tccttccatc
    17041 atggttggtt cagtttgtgg tgcagtaact gcggcgctgg tcggtgcgca gtgctatgca
    17101 ggctggggtg gtctgattgt gctgccggtg gttgaaggta agttgggtta tatcgcagca
    17161 gtggctgtcg gggcggttgt gacggcagtc tgcgtgaacg tgctgaaaag tctggcgcgt
    17221 aaaaatggat cttctactga tgaaaaagaa gacgacctgg atttggattt tgaaattaat
    17281 taattgagga acgagccatg acgaaaatta ttgcagtaac cgcgtgtcca tctggtgttg
    17341 cccatactta tatggctgca gaggcgctgg aaagtgccgc gaaagcgaaa ggctgggaag
    17401 tgaaagtaga aacccagggc tcgattggtc tggaaaacga actgactgcg gaagacgtgg
    17461 cgagcgccga tatggtgatt ctgactaaag atatcggcat caagtttgaa gagcgttttg
    17521 ctggcaagac catcgtgcgc gtcaacatca gcgatgcggt aaaacgtgct gatgccatta
    17581 tgagcaaaat tgaagctcac ctggcgcaaa ccgcttaagt tcttctcccg ctcgcaaggg
    17641 cgggttcgct ttcccacagg agttcctcat gacgaatcgt atctctcgcc tcaaaactgc
    17701 actgtttgcc aatacccgtg aaatctcgct ggagcgtgcg ctgctttata ccgccagcca
    17761 tcggcaaacc gaaggcgaac cggtgatatt gcgccgggcg aaagcaacag cgtatatcct
    17821 tgaacatgtt gaaatttcga ttcgtgatga agaactgatt gccggtaacc gcaccgtaaa
    17881 accgcgcgcc gggattatgt cgccggaaat ggacccttac tggctgctga aagagctggg
    17941 tcaattcccg acgcgtccgc aggaccgttt tgctatcagc gaagaagata aacgtatcta
    18001 ccgcgaagag ttgttcccgt actgggaaaa acgttcgatg aaagatttca tcaacgggca
    18061 gatgacggat gaagtaaaag ccgcgactag cacgcagatt ttcagcatca accagacgga
    18121 taaaggccag gggcacatta ttattgatta cccgcgcctg ctgaatcacg ggctggggga
    18181 gctggtggca cagatgcagc aacattgtca gcaacagccg gagaatcact tttatcaggc
    18241 agcgctgtta ctgctcgaag cctcgcagaa acacattttg cgttacgccg aactggcgga
    18301 aacgatggcg gcaagctgca ctgatgccca gcgtcgcgaa gaactgttga ctattgcgga
    18361 gatctcccgc cataacgccg aacataaacc gcagacgttc tggcaggcgt gccagttatt
    18421 ctggtacatg aacatcattc tgcaatacga atccaacgcc agttcgctgt ctttagggcg
    18481 cttcgaccag tatatgttgc cgttctatca ggcatcttta acccagggcg aagatccggc
    18541 gttcctgaaa gaactgctcg aatctttatg ggtgaaatgc aacgacattg tgctgttgcg
    18601 ctccaccagc agcgcgcgtt atttcgcagg tttcccgact ggctataccg cactgctcgg
    18661 cgggttaacc gagaacggac gtagcgcggt gaacgtgctt tcgttcctct gcctcgatgc
    18721 ctatcaaagc gtacaattac cgcaaccgaa cctaggggtg cgcactaacg ccttgatcga
    18781 cacgccgttc ctgatgaaaa ccgcagaaac cattcgcctc ggcaccggta ttccgcaaat
    18841 ctttaacgat gaagtggtgg tgccagcgtt cctcaaccgt ggcgtttcgc tggaagatgc
    18901 gcgcgactat tccgtagtgg gctgtgtgga attatctatt cccggcagaa cctacggctt
    18961 gcatgacatc gcgatgttta atctgctgaa agtgatggaa atctgcctgc atgaaaatga
    19021 aggcaatgct gcgctgactt atgaaggttt actggagcag atccgcgcca agatcagcca
    19081 ctacatcacc ctgatggttg agggcagcaa tatttgtgat atcggccatc gcgactgggc
    19141 acctgtaccg ctgctctcgt cttttatcag cgattgtctg gaaaaaggtc gcgatattac
    19201 cgatggcggc gcgcgttata acttctccgg cgtacaggga attggtatcg ccaacctgag
    19261 cgattctctc catgcgttga aagggatggt ttttgatcaa cagcgtttaa gttttgacga
    19321 attgctgtcg gtattaaaag ccaactttgc aacgccagaa ggcgaaaaag tccgcgctcg
    19381 cttaattaac cgctttgaga aatacggtaa cgatatcgac gaggtgggta acattagcgc
    19441 cgaactgttg cgccactact gcaaagaagt ggaaaaatac cagaacccgc gcggcggtta
    19501 cttcacgccg ggatcgtata ccgtttctgc tcacgtcccg ttgggatcgg tggttggcgc
    19561 gacgccagac ggtcgttttg ccggagaaca gttggcagac ggcggcttgt cacccatgct
    19621 gggccaggac gcacaagggc caacagcggt actgaagtca gtcagtaagc tcgataacac
    19681 gctgctgtct aacggtacgt tgctgaacgt gaaattcact ccggcgaccc tggaaggtga
    19741 agcgggatta cgcaaactgg ccgacttctt acgggcgttt acccagctta agttgcagca
    19801 tattcagttt aacgtggtga acgccgacac gttgcgggaa gcgcaacagc gcccacaaga
    19861 ttatgccggg ctggtggtgc gcgttgccgg atacagcgcc ttctttgtcg aactgtcgaa
    19921 ggagatccag gatgacatca tccgccggac agcgcatcag ctgtaacgtt gtggaaacgc
    19981 gccgcaatga tgtggcgcgc attttcaaca ttcagcgtta ttcactgaat gacggcgagg
    20041 gcattcgtac ggtggtcttt tttaaaggct gtccgcatct ttgcccgtgg tgtgctaatc
    20101 cggagtcgat ctccggcaaa atccagacgg tacgcagaga ggcgaaatgt ctgcactgtg
    20161 cgaaatgttt gcgtgatgcg gatgaatgcc cctccggggc gtttgaacgg attggtcgcg
    20221 atatcagcct tgacgcgctg gaacgggaag tgatgaaaga tgacattttc tttcgcacgt
    20281 ccggcggcgg cgtcacgctt tctggcggcg aagtgttaat gcaggcggag tttgctaccc
    20341 gttttttaca gcggctgcgg ttgtggggcg tgtcatgcgc cattgaaact gccggagacg
    20401 cgccagccag caagctgtta ccgctggcga aattgtgcga tgaagtgttg ttcgatttaa
    20461 aaattatgga cgcgactcag gcgcgggatg tggtgaagat gaacctgcca cgcgtgctgg
    20521 agaatctgcg tttgctggtg agtgagggcg tcaacgtgat cccgcgttta ccgctgatcc
    20581 ctggtttcac gctcagccgg gagaatatgc agcaggcgct ggatgtactg atcccgctga
    20641 aaatcaggca gatccatctg ttaccgtttc atcagtacgg cgaaccgaaa taccgcctgc
    20701 tggggaaaac atggtcgatg aaagaggtgc ctgcgccgtc gtcagccgat gtgacaacga
    20761 tgcgcgaaat ggcagaacgg gccggatttc aggttaccgt gggaggttaa aatggcatac
    20821 ctggtggcag taaccgcctg cgtaagcggc gtggcgcata catatatggc ggcggaacgg
    20881 ctggaaaagt tgtgcctgtt agagaagtgg ggagtcagca ttgaaactca gggcgcgctg
    20941 ggaacggaga atcgtttagc ggacgaggat atccgtcggg cggatgttgc tctgttgatt
    21001 acggatatcg agcttgccgg tgccgagcga tttgaacatt gccgctatgt gcaatgcagc
    21061 atctacgcat tcctgcgtga gccgcagcag gtaatgagcg cggtgcgcaa agtgctttct
    21121 gcgccgcagc aaacccatct tattctggag tagtcggttt ttctgtcagt tggctgtggt
    21181 actgccggcg atattccgac ggcgagcgtt cggtgttttt acgaaacaga cggcagaagt
    21241 agttgctgtc gacaaagccg caggcgtgcg ccacttcttt taccttcagg tcatagcctt
    21301 tcaataacgt cttagcgtgc tccagtcgcg tgtgattcag gtattcgtta aaaccaatgg
    21361 cccccgtttt ttgaaacagg tgcgagagat agtttggtga aatatagaat gcctgtgcga
    21421 cagattcgcg ggtaagcgcg gaggcatagc gttcgtcgat ataatcgcga atagcttcga
    21481 atagtgcctg gctgcgtgag gcggtctgga tttggctgcc aagcaaatcg cggcagtggc
    21541 taagcaggct ggcgacgata agccttgccg tttgctgctc ctgcggctgc atttgcattt
    21601 cattgagcgt ttgtagcaga aaagaaccta tgcgtgggcc gcgtcgggcg acgtgttgct
    21661 tcgccagatt ttgatattgt ttgccatccc attgcacgac gctgaagccg agttgctgtt
    21721 tgccaaacag cacgctaaag gtagtagcag gggcttgcca ttgtggaaaa ttccagccac
    21781 cagcggcaac gtacagcaca tcgccgggaa ctaacgttgc tccagcgccg gtatcaacaa
    21841 actcaccttc cagcacaatt tccagccgtg gaaaatcgac ctgatacgca agatcgggca
    21901 ccgggccgtt actgctggca aagtaaatct ggcgcaggga cagcgggccg ttgatcaggc
    21961 gggagagcag atagctgacg tcgtgataca tgttattccc ttaggtatgg agacgggaaa
    22021 acagtaactg gcattattaa aaaaggccac ctgaaaagtg tggcctgagg ggagttcgat
    22081 gtaaacattg cggcagttgt attgccggat gtggcgtaaa cgccttatcc agtctaccgc
    22141 cgcgatctgt aggccggata agacgcgtca agcgtcgcat ccggcaaata gcgcctggct
    22201 gataattact gattacctac ctggtcgcca tacggcagcg tgtcgtaatc gatcagcgcg
    22261 tttttcttgt acgggttacc aatccagcgg gtattttctt tgaactgcgg attcaccact
    22321 gaacgggttg gatcgtaacc gtcgtaagat aagcccgcta aatctgacca ggtgtggatc
    22381 agttccgcaa ggctgtattt acgatcaaca tcctgcgaga aatcacgcgg atgagtcgct
    22441 tgccattttt ccgacgtcca cagcaggaac ggaatggtgt acatatgacg cgtcgggttg
    22501 tcctcattac gcccctgggt tttatgaggc ggcgtgtcgt aaacctcttc accgtggtca
    22561 gagaagtaaa ccaggaaacc gttcgggtct gctgctttaa agtctttaat caggctggca
    22621 accacatgat cgttatacag gttagcgttg tcataatcgt tatatgactc cagctcttcc
    22681 gcgtttaatc ccggcggaac atgatcggta ttgccatcaa acttgccctg attttccggg
    22741 tagcggtatt tgtatttgat atgcgtaccc agcagatgaa cgatgatcag tttcttcggc
    22801 gcagggtcct tcagcacttc ctggaacggc ttcagcacgt tagtgtcgta ttcacgcgca
    22861 ctctgcgtac gttgctggtt catgtagtac tgcttgtcgg tctggcgcga aaataccgtc
    22921 agcatggtat tgcgggcggt catcgtctgc tggttggtga tccagaaggt tttataaccc
    22981 gcctgtttca tcatgttcat cagcgacggc tgcgtcagat acagatccgg gttcttttca
    23041 ttggcaaagg tcagcgcctg ttgcaggatt tcaatggtgt acggacgaga cgtaactacg
    23101 ttattaaaca cggtcagatt cggatcggtt ttatgcagcg cattcagctc cggcgtggtt
    23161 tcacgcggat aaccgtacag actcatgcgt ccacgctggg ttgactcgcc aatcaccagc
    23221 actaaggtgc gcggttcgtt acccgattca tctttgaaat tagccagcgg cggcaaggca
    23281 ttattttcat tcagtagctt tgttagcgag tttagttgct gacgatactg ataatagccg
    23341 gtcaggaatt gccacggtgc ggcaggctcc atacgcgagg ccaggttatc caacgttttc
    23401 tcaaacggct tgtttttgat aaacatattc atggcgatcg gatgcagaat caagccataa
    23461 agcagggcaa aagagacaac ataacgccac ggctttggaa tatagaccgg gcgcaggcgt
    23521 gtccacagca gaactgccac cgccgtatag gccagcgcga taagcacaat tttcaggctg
    23581 aaatactggc ttaaatactc gctggcttcg ttggtgttgg tttcgaacat cacaaacaga
    23641 acgctctgcg agaactcctg accgtagatg acgtagtagc acagcgccgc cagagaggcc
    23701 gcccatagca ccacgccgat cactgcggca ataattttga tccgcttcgg aaagaggaat
    23761 accggaatta accacagcga gctgaataac agcgagtcgc gaatgccgtt ggtgccacta
    23821 taaccactga tgtaaataat ggcctgtagc agagtagaga aaaaccaaaa gtagagcaat
    23881 gcccaaccca gggctttcca gctaaaaaga ggtttagcct ggacttctgt ggaatgcata
    23941 gtaagaacct gtcttgaaaa aaatatcgcc gaatttaccg acaattcctt aaggatatct
    24001 gaagggatat ccagaatttg aataaaatgc agacagaaat atattgaaaa cgagggtgtt
    24061 agaacagaag tatttcagaa aaccctcgcg caaaagcacg agggtttgca ggagaggaag
    24121 attagccggt attacgcata cctgccgcaa tcccggcaat agtgaccatt aacgcctgct
    24181 cgacgcgagg atccggttcc tggccttctt tttctgcctg gcgggagcgg tgcagcaact
    24241 cggcttgcaa tacgttcagc gggtcggtgt aaatattccg tagctgaata gactctgcaa
    24301 tccacggcag atcggccatc agatgggaat cgttggcaat cgccagcacc actttgatgt
    24361 cttcttcttg cagattgcgt aactctttac ctaacggcca cagtgttttg tctaccaggc
    24421 gttggtcata gtattccgcc agccacaggt ctgctttggc gaagaccatc tccagcatgc
    24481 cgagacgcgt cgagaagaat ggccaatcgc ggcacatagc ctccagctcg ctctgtttgc
    24541 catcttcgac cactttttgc agcgccgtac ctgcacccag ccaggcgggg agcatcagac
    24601 ggttttgcgt ccaggcgaag atccacggaa tggcgcgtag tgactcgacg ccgccggttg
    24661 ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc cagttcctgt tccggcgtag
    24721 cggagcggaa gtaaggcaca aaatctttgt tttcacgtac gtagccacgg tagagatcgc
    24781 aggagatgac tgatagttca tccataatgc gacgccagct ctctttcggc tcaggtggtg
    24841 gcagcaggtt ggcttccagt atcgccccgg tgtaaagcga caggctgctg acggtgattt
    24901 ctggcagacc atatttaaag cggatcatct cgccctgttc agtcacgcgc aggccacctt
    24961 tcaggcttcc tggcggttgt gacagcagcg ccgcatgagc aggtgcgccg ccgcgaccaa
    25021 tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc cgctttttcg caggttttga
    25081 ttaatgcatc ctgtgcctga tattgcgccc aggacgctgc catcacgccc gcatcttttg
    25141 ctgagtcgga atagccaatc atcaccatct gtttgccctg aatcaggccg cgataccagt
    25201 cgatattcag caactgagtc atgacatcgt tggcgttgtt caggtcatcg agagtttcaa
    25261 acagcggagc aaccggcatc gcaaacccga tacccgcttc tttcagcagc aggtggacag
    25321 ccagcacgtc ggacggcgtt ttcgccatcg agatcacgta ggcggcaatg gagccttgcg
    25381 gtgcttcggc aatcacctgg caggtatcga gcacttcgcg cgtttcggcg cttggttgcc
    25441 agttgcgcgg cagaagcgga cgtttggagt tcagttcgcg gatcaggaac gcctgtttgt
    25501 cggcctctga ccagctttcg tagtcgccga taccgaggta gcgggtcagc tcgcccagcg
    25561 cttcggtatg acgcgtgctc tcctgacgga tatcaatacg gaccagcggt acgccgaaac
    25621 atttcacgcg gcgcagggtg tcgagcagat cgccgttggc gataataccc atgccacacg
    25681 cctgaagtga ctggtagcaa gcgtagagcg gttcccacag ttcttcgttt tgtgtcagca
    25741 ggccttctgg ttttggcagt tcttcgcctt tcaggcgcgc ttccagccat gcctgtgtcg
    25801 ccatcaggcg agaacgcagg tttttcatca gatagcgata cggttctgcg gcaccttctt
    25861 cgccaaccag cgccagcagt tcaggggtcg cttcaaccat cgacagttca gaaaccagaa
    25921 cctgaatatc tttcaggaac agatcggtgg ctttccagcg gctgagcagc aggacgtggc
    25981 gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc accgcccatc cacgaggtaa
    26041 aacgtaccgg aacaaattcg acgggcagtt tgtagccgag gttctcttcc agttgttcgt
    26101 tcagttcgcg caggtaattc ggtacgcctt gccacaggct gttttctact acggcaaagc
    26161 cccatttggc ttcatctacc gggcttggac gcagcttacg gatttcatcg gtatgccatg
    26221 actgggcgat caactggcgc aggcgacgca tcagctggtt gtgttcgtag tcagcgatat
    26281 ctttgttatc gagctgtttt aagcaggcgt tcacttccac cattttgtgg atcagtgtac
    26341 gacgggtaat ttcggttggg tgagccgtga ggaccagttc cagcgacagc gattccactg
    26401 cttttttgat ggtgtcttcg ctcagttccg gctggttttt cagtttacgc agggtgcggg
    26461 cgatcacttc cgggttgctg gcagcttcgc ctttcggcga aatgctgtgg tattgctcgg
    26521 cggtgttggc caggttcagg aactgactaa acgcacgcgc aacgggcagc agctcgtcgt
    26581 tcgacaaatt ttgtaaggtg gtgagtaact cctggcggtt agcatcattg ccagcgcgtg
    26641 aagatttgga caacttacgg atagtttcta cgcgttcaag aatgtgttct cccaacgcat
    26701 ccttgatggt ttctcccagc actttgccga gcatactgac attactacgc aatgcggaat
    26761 attgttcgtt catattaccc cagacacccc atcttatcgt ttgatagccc tgtatccttc
    26821 acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg tcgtctttta taaagccacg
    26881 taaaagcggt gacgtcaaat gctgcgaaat cgcttcagca aacgaataaa tagcaggaat
    26941 ttacgtcatt aaattcacga cgctttaaat aagcgtaact tatgggaatg ttaaaaaatc
    27001 gcctcaagta acaccaaagg tgtaggtcgg ataagatgtg caagcatcgc atccgacatt
    27061 attgcggcac tggagtttgg caacagtgcc ggatgcggcg caagcgcctt attcggccta
    27121 cgttttaatg ccagcaaaaa tggtgaatga cctgggttat cagttcgcgg gtgggcttga
    27181 taaaccgtgt ttccagatat tcatcaggct gatgagcctg attaattgag ccaggcccca
    27241 gaaccagcgt cgggcataac gtttgaataa acggcgcttc ggtacagtaa ttcaccactt
    27301 cggtttttgc tccgagcaat ttctcaacca cttcaaccag ttgatgattc ggtgggcatt
    27361 catagccagg gatcggcgga tgcagctcgt cgaccgtcag acgacccggc cagcgttcgc
    27421 tcaccggagc caatgcatcg ttgagcaaac cattaagttc attgagtgtc atgccaggca
    27481 gcggacgaat atccatatgc aactcacacc aagcgcaaat acggttagaa gcgtcgccac
    27541 cgtgaatatg cccgaggttg agcgtagggt atggcacggt aaacgcttcg tagtgataac
    27601 gttctttcag gttatcgcgc aattgcaaaa tatgcccgat ggcgtcgtgc attagttcta
    27661 tagcgttaac tccgcgtgct ggatcgctgg agtgccccga ctggccctga atacggatgg
    27721 cgttagagat atgaccttta tgtgcgcgta ccggttgtag tgacgtcggt tcgccaatga
    27781 tggcgcaatc cgggcgcagg gcggtagttt cggcaaaata acgcgctccg gccatactgg
    27841 tttcttcatc agcagtcgcc agaatgtaga gcggtttttt cagtttcgtg acgtcgacat
    27901 cgcgtagcgc atcaaggata aacgcaaaaa agcctttcat gtcggcggtg cctaagccgt
    27961 aaagcttgcc gtcatgctcc gtcagtgtaa acggatcgcg cgtccagcga ccgtcatcaa
    28021 atggcaccgt atcggtatgc cccgccagca acaagccgcc agccccctgt ccggtacttg
    28081 ccagcatatt aaatttgttg cgagttcctg gtacaggctg cacttccaca ttgaagccta
    28141 aatctttaaa ccagtccgcc agcagagtga ttaaatctgc attgctttga tcgagtgcct
    28201 cttccgtggc gcttattgaa ggtgtggcaa tcagagcacg gtaaatctcg ataaatggcg
    28261 gtaatttgtt tttcattgtt gacacacctc tggtcatgat agtatcaata ttcatgcagt
    28321 atttatgaat aaaaatacac taacgttgag cgtaataaaa cccaccagcc gtaaggtgaa
    28381 tgttttacgt ttaacctggc aaccagacat aagaaggtga atagccccga tgttgaatac
    28441 gctgattgtg ggtgccagcg gctacgctgg cgcagagcta gtgacctgtg taaatcgcca
    28501 tccgcatatg aacataaccg ctttgactgt ttcagcgcaa agcaatgatg cgggaaagtt
    28561 aatctccgat ttgcatccgc agctaaaagg catcgttgat ctgccgttgc agccgatgtc
    28621 ggatatcagc gagtttagcc ctggggtgga cgtggtgttt ctcgccaccg cccatgaagt
    28681 tagccacgat ttagcgccgc agtttcttga cgcgggctgc gtggtgttcg acctttccgg
    28741 cgcgtttcgt gttaacgacg ccaccttcta tgaaaaatat tacggcttta cccatcaata
    28801 cccggaactg ctggaacagg cagcctacgg tctggcggag tggtgcggta ataaattaaa
    28861 agaagcgaat ttgattgcgg tgccaggctg ttatccgacg gcagcacagt tggcgctgaa
    28921 accgttgatt gatgccgatc ttcttgacct caatcagtgg ccggtgatca acgccaccag
    28981 cggcgtgagc ggtgcaggac gtaaggcagc gatttcaaat agtttttgtg aagttagcct
    29041 gcaaccgtat ggcgtcttta cccatcgcca tcaaccagag atcgccacac acctcggtgc
    29101 tgacgttatc ttcaccccgc atctgggcaa tttcccgcgc ggcattctcg aaaccattac
    29161 ctgccgcctg aaaccgggtg tgacccaggc gcaagtggcg caagcgttac agcaggcgta
    29221 tgcccataaa ccgctggtgc ggctgtatga caaaggcgtt ccggcgctga aaaatgtcgt
    29281 tgggctacca ttttgcgata tcgggtttgc cgttcagggc gaacatctga ttattgtggc
    29341 gaccgaagac aacttactga aaggcgcggc ggcacaagcg gtacagtgcg ccaatattcg
    29401 tttcggctat gcggaaacgc agtctcttat ttaagggtgc aatgatgaat ccattaatta
    29461 tcaaactggg tggcgtactg ctggatagtg aagaggcgct ggaacgtctg tttagcgcac
    29521 tggtgaatta tcgtgagtca catcagcgtc cgctggtgat tgtgcacggc ggcggttgcg
    29581 tggtggatga gctgatgaaa gggctgaatc tgccggtgaa aaagaaaaat ggcctgcggg
    29641 tgacgcctgc tgatcagatt gacattatca ccggagcact ggcgggaacg gcaaataaaa
    29701 ccctgttggc atgggcgaag aaacatcaga ttgcggccgt aggtttgttt ctcggtgacg
    29761 gcgacagcgt caaagtgacc cagcttgatg aagagttagg tcatgttgga ctggcgcagc
    29821 caggttcgcc taagcttatc aactccttgc tggagaacgg ttatctgccg gtggtcagct
    29881 ccattggcgt aacagacgaa gggcaactga tgaacgtcaa tgccgaccag gcggcaacgg
    29941 cgctggcggc aacgctgggc gcggatctga ttttgctctc cgacgtcagt ggcattctcg
    30001 acggcaaagg gcaacgcatt gccgaaatga ccgccgcgaa agcagaacaa ctgattgagc
    30061 agggcattat tactgacggc atgatagtga aagtgaacgc ggcgctggat gctgcgcgca
    30121 cgttgggccg tccggtagat atcgcctcct ggcgtcatgc ggagcagctt ccggcactgt
    30181 ttaacggtat gccgatgggt acgcggattt tagcttaagt tttgttggcc ggaggcgcag
    30241 ctttccggca ttgaatttca aaataaggaa acagagttat ggcactttgg ggcgggcgtt
    30301 ttacccaggc agcagatcaa cggttcaaac aattcaacga ctcactgcgc tttgattacc
    30361 gtctggcgga gcaggatatt gttggctctg tggcctggtc caaagccctg gtcacggtcg
    30421 gcgtgttaac cgcagaagag caggcgcaac tggaagaggc gctgaacgtg ctgctggaag
    30481 atgttcgcgc caggccacaa caaatccttg aaagcgacgc cgaagatatc catagctggg
    30541 tggaaggcaa actgatcgac aaagtgggcc agttaggcaa aaagctgcat accgggcgta
    30601 gtcgtaatga tcaggtagcg actgacctga aactgtggtg caaagatacc gttagtgagt
    30661 tactgacggc taaccggcag ctgcaatcgg cgctggtgga aaccgcacaa aacaatcagg
    30721 acgcggtcat gccaggttac actcacctgc aacgcgccca gccggtgacg ttcgcgcact
    30781 ggtgcctggc ctatgttgag atgctggcgc gtgatgaaag ccgtttgcag gatgcgctta
    30841 agcgtctgga tgtcagcccg ttaggttgtg gcgcgctggc gggaacggct tatgaaatcg
    30901 accgtgaaca gttagcgggc tggctgggat ttgcttcagc aacccgtaac agtctcgaca
    30961 gcgtttctga ccgtgaccac gtgctggaac tgctttctgc tgccgctatc ggcatggtgc
    31021 atctgtcgcg ttttgctgaa gatctgattt tctttaacac cggcgaagcg gggtttgtgg
    31081 agctttctga ccgcgtgact tccggttcat cattaatgcc gcagaagaaa aacccggatg
    31141 cgctggagct gattcgcggt aaatgcggtc gggtgcaggg cgcgttaacc ggcatgatga
    31201 tgacgctgaa aggtttgccg ctggcttaca acaaagatat gcaggaagac aaagaaggtc
    31261 tgttcgacgc gctcgatacc tggttggact gcctgcatat ggcggcgctg gtgctggacg
    31321 gcattcaggt gaaacgcccg cgttgccagg aagcggcgca acagggttac gcgaacgcca
    31381 ctgaactggc ggattatctg gtggcgaaag gtgtgccgtt ccgcgaggca caccatattg
    31441 tgggtgaagc tgtggtggaa gccattcgtc agggcaaacc gctggaggag ctgccgctca
    31501 ccgagttgca gaaatttagt ccggtgattg gtgaagatgt ctatccgatt ctgtcgctgc
    31561 aatcgtgcct cgacaagcgt gcggcaaaag gcggcgtctc accacagcag gtggcgcagg
    31621 cgattgcttt tgcaagggcg cggttggggt aagaacattt atatgtacaa atttgagcct
    31681 ggcttatcgc cgggcttttt tatggcaaaa aaaagcggat cctggagatc cgcaaaagtt
    31741 cacgttggct ttagttattc gagttgagaa actctcgaaa cgggcagtga cttcaagggt
    31801 taaaagaggt gccgctccgt ttctgtgagc aattatcagt cagaatgctt gatagggata
    31861 atcgttcatt gctattctac ctatcgccat gaactatcgt ggcgatggag gatggataat
    31921 gaatattcgt gatcttgagt acctggtggc attggctgaa caccgccatt ttcggcgtgc
    31981 ggcagattcc tgccacgtta gccagccgac gcttagcggg caaattcgta agctggaaga
    32041 tgagctgggc gtgatgttgc tggagcggac cagccgtaaa gtgttgttca cccaggcggg
    32101 aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag gtgaaagtcc ttaaagagat
    32161 ggcaagccag cagggcgaga cgatgtccgg accgctgcat attggtttga ttcccacggt
    32221 tggaccgtac ctgctaccgc atattatccc gatgctgcac cagacctttc caaagctgga
    32281 aatgtatctg catgaagcac agacccacca gttactggcg caactggaca gcggcaaact
    32341 cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca ttcattgaag tgccgttgtt
    32401 tgatgagcca atgttgctgg ctatctatga agaccacccg tgggcgaacc gcgaatgcgt
    32461 accgatggcc gatctggcag gggaaaaact gctgatgctg gaagatggtc actgtttgcg
    32521 cgatcaggca atgggttttt gctttgaagc cggggcggat gaagatacac acttccgcgc
    32581 gaccagcctg gagacgctgc gcaacatggt ggcggcaggt agcgggatca ctttactgcc
    32641 agcgctggct gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc cgtgcattaa
    32701 gccggaacca cgccgcacta ttggcctggt ttatcgtcct ggctcaccgc tgcgcagccg
    32761 ctatgagcag ctggcagagg ccatccgcgc aagaatggat ggccatttcg ataaagtgtt
    32821 aaaacaggcg gtttaaaccg tttaacgcag ctacccgata ggcttccgcc atcgtcgggt
    32881 agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc gccacctttc tgttccataa
    32941 tcgcctgacc gatatgaata atttcggcag cgcgctcgcc aaagcagtga atgcccagaa
    33001 tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc cacgttcatg ccgacgattt
    33061 gtgcacgtgc cagatgtttg aactgggcgc ggcccacttc atatggcact ttcatcgcgg
    33121 tcagctgctg ttcggttttg cccacagagc tgatttccgg gatggtgtaa atgccggtag
    33181 ggatatcttc aatcagatgc gcggtggctt cgccttttac cagcgcctgc gcggcaatgc
    33241 gcccctggtc ataggccgct gatgccaggc tcggataacc aatcacgtcg cccaccgcgt
    33301 aaacgtgcgg ctgtgcggtc tgatacatgc tgttgacctt cagctgtccg cgactgtcag
    33361 tttccagccc aatgttctgt aacgccagcg aatcggtatt accggtacga ccgttggcat
    33421 agagcaggca gtcagctttc agttttttac ccgacttcag atgcatgatc acaccgtcgt
    33481 cacagccttc gatcttctcg tactcttcgt tgtgacgaat cactacgcca ctgttccaga
    33541 agtgatagga gagagaatct gacatctctt gatcgagaaa tgccagcagg cgatcgcggg
    33601 tgttgatcag atccactttt acatccatac cgcggaagat cgacgcatat tcacagccga
    33661 tcactccagc accatagata agtacatggc gcggttcgtg gtgcatgctg agaattgagt
    33721 cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt tggatgatat ggacgagagc
    33781 cgcaggcaat aacaaatttt tcagcggtta gtgtttcaac gctgccgtcc gggcaatcca
    33841 gcgccaacgt atgctcgtca acaaagcgag cgttcccctg caatatttca cagtgattac
    33901 gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat cacgttatcg gcatggttaa
    33961 ggatatcggc aaaagaagag cggagcagtc gggaatggtc gctgtatagt gggttttgat
    34021 tgaattctat aatgcggctg acggcgtgac ggagagcttt cgacgggatg gtgccccagt
    34081 gggtgcaacc gccgccaaca ttttgataac gctcgataac tgcgacgcgc gcaccttgct
    34141 taaccaggcc cattgcagcg ccttcgccgc cggggccgga acctattact atggcatcgt
    34201 aatcgtagga atgtggcatg gtagggctta cctgttctta tacataaaag caacagaatg
    34261 gtaacatttt atcgcgggta agccaattga tccccgtcat taatctggct atagcgtgag
    34321 cggcctttgc tttgtctgcc tccttcttaa cccatttatt cactattaat cgctttttat
    34381 tatggctgat agttttatct tgccgaagcg tgcttttttg ttattgtctt tgtgaaatct
    34441 tcttataaaa caaacatata aatattttat attgatgagt tatttgtatt tacaccatca
    34501 ataaaatccg ttaattagcc tgtttataag tgaataaagg aatagccgat gatcgaacac
    34561 tatattcgag ggtttgagga agaactgcgt gagatccgcc atcagatcca cgaaaacccg
    34621 gagctgggat tacaagaatt taaaaccagt gctttggttg cggaaaagct gcgtcagtgg
    34681 ggctatgaag tagagcaagg actggcgacg acggggatag tggccacgtt aaaagtgggc
    34741 gacggagaga aaagtattgg cctgcgggcg gatatggatg cgctgccgat ttatgaaaat
    34801 agtggcaagc cctgggccag taaacatcct ggtttaatgc acgcctgcgg gcatgacggg
    34861 cataccacca tactgctggg ggccgcgcgt tattttgccg aaacccgccg ttttaacggc
    34921 acgttacgtc ttatcttcca gcctgcggaa gagatgatta atggcggtga gatcatggtt
    34981 aaagaggggc tttttgatca tttcccctgc gatgtcattt tcggcatgca taacatgccc
    35041 ggtctgccgg tgggtaagtt ttatttccag ccaggggcac tgatggcgtc aatggatcag
    35101 ttccatatta cagttcgtgg ttgtggcgga cacggtgcga tcccgcacaa agccatcgat
    35161 ccggtgctag ttgccgcaca tatcaccacc gcattacaaa gcattgtgtc acgcaatgtc
    35221 gatccgctgg aagcggcggt aattaccgtc ggcagcattg ttgcgggtga ggcggctaac
    35281 gtcattcctg acagtgctga gatgaaaatc agcgtccgct ctcttagccg cgatacccga
    35341 caacttcttc tgacccgtat tcctgctctg gcacaagccc aggctgccag ctttggcgct
    35401 acggctgagg ttacgcatgt taacggtaca ccagtattag taaatgacga ggagatggcg
    35461 cgcttcgcct ggcaggtggc gtgtaaaacg tttggtgaag atcgggccga gtttggcatc
    35521 aagcccctga tgggcagtga agatttctct ttcatgctgg aagcccaacc caaaggcggt
    35581 ttcctgttat tcggtaatgg cgatgtcggg gaaggttcct gcatggtgca taacccgggt
    35641 tacgacttca acgatgcaag tctggtcccg gcaagcagct actggggcgc actggtagaa
    35701 gcctggctgc aataaataaa acaacacaca acaacacttt ttggcaagga acatggaatg
    35761 acggcgatcg taaatgaaac cgtgagtgtg aagaaaaagc ccagcggcag gcgggtgatt
    35821 tttgcctcgg cgtttggcaa tgcgctcgaa ttttttgatt ttggcgttta caactttttt
    35881 gtggtctata tcagcacgct attttttccg cccagtgcag ataaaaacgt cgcgctactg
    35941 ctggcgtttg ccactttcgg cgtgagcttt tttatgcgcc cgctcggcgg cattattgtg
    36001 ggggcctggg ccgatcgttt cggacgcaaa cccgcgatgg tgttcaccat tgcgctgatg
    36061 agtcttggta cactgatgat cggcatcgca ccgacgtatg aaacggcggg ctattgggga
    36121 accgcgacgc tggtgctagc gcgtctaatt cagggcgttg cggcaggagg cgaagtcggt
    36181 gcctcaatgt ctttgttggt tgaatcagct ccggcaaatc gtcgcggttt ttacagcagt
    36241 tggtcactgg cgactcaagg cctggcaaca acttttggag gtgttgtcgc tttaggttta
    36301 agtgcatggc tgccctttgc gaccggttca gaaaccgtca tggctgaatg gggctggcgt
    36361 gtgccgtttt ttattggcgt gttattagcg cctgtcggct gttggttgcg tttgagtctg
    36421 gaaaatgatg ttcctgagcc agcacataat aagaaggccg cagccagcga aagcgccttc
    36481 tccttactta tgcaacataa agcgactatc gctaacggta tcttgctcgc tattggtagt
    36541 acagtcgcaa cctatatctc cctcttttat tacggtacat gggcggcgaa gtatttagga
    36601 atgaaccaaa actattctca tgcagcgatg ttactggcgg gcgttattac gtttgtgggt
    36661 gcgctgctgg tggggatgtt gtgcgattcg gtcgggcgta aaaagctgat tttaatctcc
    36721 cgtgtgatgg tgctgatctg tagctggccg tcattctggc tgttggtgaa ttacccaagc
    36781 ccaggcatgt tgctgacggt ggttttcgtg atggtcagct ttaccacgct tggcggtgtg
    36841 ccagtgatgt tgttgatctc tgaactgttg ccgaaacgga ttcgggcatt gggttttgcg
    36901 ctggtttata gcattggtgt ggcaatcttt ggcggttttg ctcaatattt cgctacgcaa
    36961 tccatcgtgt tgctggatag tttgacggct ccggcgtggt atctgggtgg aggcacgttg
    37021 ctgtcgatgt tggcgttgtt gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt
    37081 tttattgcgt taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta
    37141 tagtgccagc agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca
    37201 aaaagaaaaa acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg
    37261 cagcttcgcc agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc
    37321 tttttatcgg catttccgag acgtagacga actgggtctg accatggttg atgagagcgg
    37381 tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt
    37441 gatccgcacc tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg
    37501 gttattattg cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga
    37561 aattcagcac ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg
    37621 tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga
    37681 ggcgttggac gtcggcgttg aacaacgtcg gcaattagaa gagcgactgg tactgcaact
    37741 gcgaatgatt tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat
    37801 tattccggga aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg
    37861 ctgctggcgt tggttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc
    37921 attgtgccat tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat
    37981 caacgttatc ttaatcgcac catgccggta ggcttgccgg gtctggcagc tgcctgtttt
    38041 attctcggcg tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct
    38101 aacttcttcc cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt
    38161 aaccgtaagc aggaagttgc tgagtaatcg gtatcatgcc ggatgctggc gcatccggca
    38221 tgggttttac ttcgcggtca gtaatacgct gcactccata tggtgcgtgt aggggaactg
    38281 atcaaacaga gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gatttttgca
    38341 taacgtttcc ggattgcagg agatatacag aatacgtgga tacgcctgca ccattttctc
    38401 ggtttcactg tccagaccgc tgcgaggtgg gtcgacaaaa atggtttcgc actgataact
    38461 ctttaagtcg atcccttgca ggcggttaaa ctcgcgcacg ccattcatcg cctgagtaaa
    38521 ttcttctgcc gccatacgaa taatttgtac gttatcaata tggttagcgg caatgttgta
    38581 ttgcgcggca gcgactgatg gcttggcgat ttcggtggct aatacccgat caaaattgcg
    38641 cgccagcgct aatgagaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga
    38701 gcctttggtt acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg
    38761 ggtaaagctg ttttctacct gacgatagat catctctttc cctgcgaccg gcagacgctc
    38821 gtcgatgtaa tcttgatcca gcgcgatttt ggttttcgtt gcccgaccaa tcagatgcac
    38881 attcagattc tgcgcgcgca gtgcatcgcg cagggcttcc gcttcctgac gccactcatc
    38941 atccagcttt ttatggtaca gcagggaaac caccgcctga ttactcagcg tggtgaggta
    39001 atcaatctgg aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc
    39061 cgtcatcaac tggttgatga gttcactggc ggcggggaag ctatccaccc ggatacggct
    39121 tttggtttgt tgatcgaaaa tgatgtggta caggtcatcg ccatcgtgcc agatgcggaa
    39181 ctccgcacgc atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga
    39241 aaacggtgcc atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg
    39301 ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat
    39361 tgtagggatt gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct
    39421 ggacagtaac ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa
    39481 tccagtgcga atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg
    39541 cggacactgc cattcggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc
    39601 ccgaagacct gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac
    39661 ctgcggcatc cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga
    39721 cggcgtgttc tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg
    39781 tcgttactgc taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg
    39841 tgacgcgtca ggatatcgac cgctggcagt cgacctcggt taatgatgtg ctgcgccgtc
    39901 ttccgggcgt cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc
    39961 gcggtacaaa tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg
    40021 gggggagtgg ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat
    40081 atatccgtgg gccacgctcc gccgtttatg gttccgatgc aataggtggg gtggtgaata
    40141 tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt
    40201 atcaaaacta tgatgtctct acacagcaac aactggggga taagacacga gtaacgttgt
    40261 tgggcgatta tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc
    40321 aagcgcagcc agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata
    40381 actttactga tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt
    40441 atgacgcgta ttattctccg ggttcaccat tggtcgatac ccgtaaactc tatagtcaaa
    40501 gttgggacgc cgggctgcga tataacggcg aactgattaa atcacaactc attaccagct
    40561 atagccatag caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga
    40621 cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa caacatcatc attggccacg
    40681 gtaatgttgg tgcgggtgtt gactggcaga agcagagcac ggcaccgggc acagcttatg
    40741 ttaaggatgg atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg
    40801 gcgattttac ctttgaaggc gcagcacgca gcgacgataa ctcacagttt ggtcgtcatg
    40861 gaacctggca aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct
    40921 acgggacatc ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga
    40981 atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg
    41041 tgaactggcg tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc
    41101 acaccctgaa atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca
    41161 attttgatac cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg
    41221 caattaccga cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact
    41281 ggcagttgta tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata
    41341 aggattactc atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc
    41401 ttgcggttgc gtatccggtc acctctcacc tgacagttcg tggtaaaata gccaacctgt
    41461 tcgacaaaga ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt
    41521 ctggcagcta caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt
    41581 gggttgtcgg tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct
    41641 ttcgataacg tcgctttccc gtatggcgaa aaaagcgaag cgtttattgt tgagcgagtg
    41701 gtggcaattg tcaccgcggt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc
    41761 aacactgcca gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt
    41821 ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta
    41881 ctggcaaccc gcggaacagt taaacgttct tatactcatg agctgatcgc gcgtttcgct
    41941 aatgaatgcc agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag
    42001 ctgcatggcg aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga
    42061 atgaaagagc cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa
    42121 gaactgttac aagtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct
    42181 cgccgaacgg cctggttgtt agaacatgaa gccccggatg caaaatctgc cgatgcgaat
    42241 attgcctttt gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt
    42301 tacggcttcg aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc
    42361 ggtcggaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    42421 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatt
    42481 ccg
//
LOCUS       DBFIFS010000014        42348 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.014, whole genome shotgun sequence.
ACCESSION   DBFIFS010000014 DBFIFS010000000
VERSION     DBFIFS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.014"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..68
                     /locus_tag="N7A07_000739"
     CDS             <1..68
                     /locus_tag="N7A07_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475465.1"
                     /translation="NKSSLNNLVFSRFIDRLNECF"
     gene            215..1435
                     /gene="ybdN"
                     /locus_tag="N7A07_000740"
     CDS             215..1435
                     /gene="ybdN"
                     /locus_tag="N7A07_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2475466.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1408..2037
                     /gene="ybdM"
                     /locus_tag="N7A07_000741"
     CDS             1408..2037
                     /gene="ybdM"
                     /locus_tag="N7A07_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU2475467.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2038..3198)
                     /gene="ybdL"
                     /locus_tag="N7A07_000742"
     CDS             complement(2038..3198)
                     /gene="ybdL"
                     /locus_tag="N7A07_000742"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU2475468.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3307..4395
                     /gene="hcxA"
                     /locus_tag="N7A07_000743"
     CDS             3307..4395
                     /gene="hcxA"
                     /locus_tag="N7A07_000743"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU2475469.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4405..4602)
                     /gene="ybdD"
                     /locus_tag="N7A07_000744"
     CDS             complement(4405..4602)
                     /gene="ybdD"
                     /locus_tag="N7A07_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU2475470.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4784..6889)
                     /gene="cstA"
                     /locus_tag="N7A07_000745"
     CDS             complement(4784..6889)
                     /gene="cstA"
                     /locus_tag="N7A07_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU2475471.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7070..7483)
                     /gene="entH"
                     /locus_tag="N7A07_000746"
     CDS             complement(7070..7483)
                     /gene="entH"
                     /locus_tag="N7A07_000746"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU2475472.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7486..8232)
                     /gene="entA"
                     /locus_tag="N7A07_000747"
     CDS             complement(7486..8232)
                     /gene="entA"
                     /locus_tag="N7A07_000747"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU2475473.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8232..9089)
                     /gene="entB"
                     /locus_tag="N7A07_000748"
     CDS             complement(8232..9089)
                     /gene="entB"
                     /locus_tag="N7A07_000748"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU2475474.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9103..10713)
                     /gene="entE"
                     /locus_tag="N7A07_000749"
     CDS             complement(9103..10713)
                     /gene="entE"
                     /locus_tag="N7A07_000749"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU2475475.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10723..11898)
                     /gene="entC"
                     /locus_tag="N7A07_000750"
     CDS             complement(10723..11898)
                     /gene="entC"
                     /locus_tag="N7A07_000750"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU2475476.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12087..13043
                     /gene="fepB"
                     /locus_tag="N7A07_000751"
     CDS             12087..13043
                     /gene="fepB"
                     /locus_tag="N7A07_000751"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2475477.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13047..14297)
                     /gene="entS"
                     /locus_tag="N7A07_000752"
     CDS             complement(13047..14297)
                     /gene="entS"
                     /locus_tag="N7A07_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU2475478.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14408..15412
                     /gene="fepD"
                     /locus_tag="N7A07_000753"
     CDS             14408..15412
                     /gene="fepD"
                     /locus_tag="N7A07_000753"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU2475479.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15409..16401
                     /gene="fepG"
                     /locus_tag="N7A07_000754"
     CDS             15409..16401
                     /gene="fepG"
                     /locus_tag="N7A07_000754"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU2475480.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16398..17213
                     /gene="fepC"
                     /locus_tag="N7A07_000755"
     CDS             16398..17213
                     /gene="fepC"
                     /locus_tag="N7A07_000755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU2475481.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17210..18343)
                     /gene="wzz(fepE)"
                     /locus_tag="N7A07_000756"
     CDS             complement(17210..18343)
                     /gene="wzz(fepE)"
                     /locus_tag="N7A07_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU2475482.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18559..22440)
                     /gene="entF"
                     /locus_tag="N7A07_000757"
     CDS             complement(18559..22440)
                     /gene="entF"
                     /locus_tag="N7A07_000757"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU2475483.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22437..22655)
                     /gene="ybdZ"
                     /locus_tag="N7A07_000758"
     CDS             complement(22437..22655)
                     /gene="ybdZ"
                     /locus_tag="N7A07_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HGU2475484.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22658..23860)
                     /gene="fes"
                     /locus_tag="N7A07_000759"
     CDS             complement(22658..23860)
                     /gene="fes"
                     /locus_tag="N7A07_000759"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU2475485.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24103..26343
                     /gene="fepA"
                     /locus_tag="N7A07_000760"
     CDS             24103..26343
                     /gene="fepA"
                     /locus_tag="N7A07_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU2475486.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26518..27138
                     /gene="entD"
                     /locus_tag="N7A07_000761"
     CDS             26518..27138
                     /gene="entD"
                     /locus_tag="N7A07_000761"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU2475487.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27260..27412)
                     /gene="hokE"
                     /locus_tag="N7A07_000762"
     CDS             complement(27260..27412)
                     /gene="hokE"
                     /locus_tag="N7A07_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU2475488.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27763..27915)
                     /gene="hokE"
                     /locus_tag="N7A07_000763"
     CDS             complement(27763..27915)
                     /gene="hokE"
                     /locus_tag="N7A07_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU2475489.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            27884..28151
                     /locus_tag="N7A07_000764"
                     /pseudo
     CDS             27884..28151
                     /locus_tag="N7A07_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28354..29472
                     /gene="ybdK"
                     /locus_tag="N7A07_000765"
     CDS             28354..29472
                     /gene="ybdK"
                     /locus_tag="N7A07_000765"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU2475490.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29538..29786
                     /gene="ybdJ"
                     /locus_tag="N7A07_000766"
     CDS             29538..29786
                     /gene="ybdJ"
                     /locus_tag="N7A07_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU2475491.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29851..30219
                     /gene="ybdF"
                     /locus_tag="N7A07_000767"
     CDS             29851..30219
                     /gene="ybdF"
                     /locus_tag="N7A07_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU2475492.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30313..30966
                     /gene="nfsB"
                     /locus_tag="N7A07_000768"
     CDS             30313..30966
                     /gene="nfsB"
                     /locus_tag="N7A07_000768"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU2475493.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31074..32321
                     /gene="ybdG"
                     /locus_tag="N7A07_000769"
     CDS             31074..32321
                     /gene="ybdG"
                     /locus_tag="N7A07_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU2475494.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32389..33765)
                     /gene="pheP"
                     /locus_tag="N7A07_000770"
     CDS             complement(32389..33765)
                     /gene="pheP"
                     /locus_tag="N7A07_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU2475495.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33867..37004)
                     /gene="cusA"
                     /locus_tag="N7A07_000771"
     CDS             complement(33867..37004)
                     /gene="cusA"
                     /locus_tag="N7A07_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU2475496.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(37016..38239)
                     /gene="cusB"
                     /locus_tag="N7A07_000772"
     CDS             complement(37016..38239)
                     /gene="cusB"
                     /locus_tag="N7A07_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU2475497.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38255..38587)
                     /gene="cusF"
                     /locus_tag="N7A07_000773"
     CDS             complement(38255..38587)
                     /gene="cusF"
                     /locus_tag="N7A07_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU2475498.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38611..39993)
                     /gene="cusC"
                     /locus_tag="N7A07_000774"
     CDS             complement(38611..39993)
                     /gene="cusC"
                     /locus_tag="N7A07_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU2475499.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40150..40833
                     /gene="cusR"
                     /locus_tag="N7A07_000775"
     CDS             40150..40833
                     /gene="cusR"
                     /locus_tag="N7A07_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU2475500.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40823..42271
                     /gene="cusS"
                     /locus_tag="N7A07_000776"
     CDS             40823..42271
                     /gene="cusS"
                     /locus_tag="N7A07_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU2475501.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT        10116 a        11736 c        10790 g         9706 t
ORIGIN      
        1 ataataaatc ttctttaaac aatctggtgt tctcacgatt tattgaccgt ctaaatgagt
       61 gtttttgata tataacctca gacagctact ataactttca cctatttatt cacagcgcga
      121 atgaaaaata aaacactctg cttttcagtg tttactttat aattaacagt cttatctcac
      181 tgatcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
      241 gctcaatatt ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg
      301 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
      361 actcgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt
      421 ctcttgcacc attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga
      481 gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga
      541 atggcagtgc tgggaacctg atgtcgaatg ggtacgtcag cccccgcaag atgcgataac
      601 cgatcctgac tttttctgct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga
      661 gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca tccgtgcgga
      721 tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga
      781 taaaccctgg actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga
      841 ctggaaagtg gctgatatct ggacctggta tgcaaatcat caaagtctct gcaacccact
      901 gtataaccta atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt
      961 tggcccggag caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc
     1021 tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa
     1081 tcatttctat gggcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc
     1141 attattgctg ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc
     1201 catttatttg cactggtatc agaaaaaagg catcgaggtt ccacaaaccc agcaagggga
     1261 cattggcgcg aaagatatcc cctcctggcg gcggatatgc aaagttttac tcaataacga
     1321 ttactggtgt cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa
     1381 cgaacggata aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag
     1441 gatttaacgc aatttctcgc cagcttgccg gaagatgatc gcatcaacgc gattaatgaa
     1501 atccgcatgg cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg
     1561 tgggttaaaa acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc
     1621 gaaaaaaaac tgctgaaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc
     1681 acgcatacgg ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca tgaaattggt
     1741 aaaggctcat catcattaaa attacgctta aaaggttatt tgccggtgac ctgtctggaa
     1801 ggtacgcgta atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat
     1861 caaatcaccg ccatgtcgga aatcgtccgt gaactcagtc agttgggatg ggacgataat
     1921 aaaattggca aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac
     1981 ggtctgcaag agctgtttgc cgaccgtcaa tattcccgcg cctggactgt caaataacta
     2041 cagctggcgc agtcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag
     2101 acgaatcagt ttatgtggga agggatcggc gcaaaacacc gatagcggaa tcgccgccac
     2161 gccgtgctcc cgcgtcagcc actggcaaaa ctcaacatca tccagggtag aaaccgcgct
     2221 gtaatccacc agcaaaaagt atgtgccttc gcacggtaaa atctccagcc gactttcatt
     2281 taaggcattt accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg
     2341 ctcaggttct gcacgtagca tatccgcaat tgccagctgt gccggggtat tcaccgaaaa
     2401 ggtcagatac tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg caacacaata
     2461 acccactttc cagccggtca tatgataggt cttgccaaac gatgacaccg ccaccgcccg
     2521 ctcacgcagc tgcggatgcg ccagcacact ggcatggccc tgttgtgaaa agttgatgtg
     2581 ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc
     2641 ggcgaaatca gcctgctgcc agacagttgc actgggatta tgcggagtgt tgaggatcac
     2701 cagtctggtg cgctcgctta acaatgcggc aaattcctgc cagtcaacgc gaaaatgcgg
     2761 tggttgcagt gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact
     2821 gtcatagctg ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc
     2881 cgcgtataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg
     2941 atagccatac aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt
     3001 catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata
     3061 gcgcggacca tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc
     3121 cagcgcgctc atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat
     3181 cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa
     3241 cacgatgcta gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa
     3301 cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat
     3361 ccaggaagtt tcaatcacct gcacgatttt ttcactgatg aacaactttc tcgcgcggtg
     3421 tggatctacg gcgtacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga
     3481 cttccagggg caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa
     3541 ctggcggctg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcacta
     3601 ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgctccaacg
     3661 atcgccgcca cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag
     3721 gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga accggagatt
     3781 atcctcaatg cgccgcaaca atatctgctg gcggggatcg gtgatacgct ggcgaaatgg
     3841 tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg
     3901 atcaataatg ctcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcgcttgcc
     3961 gatcagcaaa atcagcagtt aacgcaatca ttttgcgatg tggtggatgc cattattgct
     4021 ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtagcggc ggcccatgcc
     4081 gtacataacg gtctgactgt cctgccacaa accgagaagt ttctccacgg caccaaagtc
     4141 gcctacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta
     4201 accggggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc
     4261 aataatcagg cggagatcga taaagtgatt gcccacacct tgcgcccggt ggagtccatt
     4321 cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa
     4381 tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg
     4441 cgcgtcctgc cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt
     4501 aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc
     4561 ctgacctaaa tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca
     4621 tgttgctgaa agagtgccgg atgcggcgtg aacgccttat ccggcctaca aatgctatca
     4681 acgcgctcgg tcagccccct cgccccatcg gggagagggt tagggtgagg gggaacccac
     4741 ggcgccatgc caacattcgc caacatcccc cctcactctg actttagtgc gcgccttttg
     4801 cctgcaccac gatctcctcg acattttccg gcattggctc atacggcgtt tctttcgccg
     4861 ttggcttcgg ctctttcaat gccgcaagtg ccgtcttaat cgagaacagt gccagaacca
     4921 ccacgaccac cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt
     4981 gcgccagctg tgactcagta tactgcgatg gaatattacc gctgtcgatc attgcctgga
     5041 acttattagc aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc
     5101 ctgcggtcag ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac
     5161 gttgacgttt catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca
     5221 tctggttggc aataccaaac agcggccaca gagtgttaat accgcccagc ggatcgacca
     5281 caccctgatg caggaagtag ccccacgcca gcacgcacag cgccgttgcc agcaggttag
     5341 caggcagtga atcggtccgt ttcaggcccg gagaaaccac gcccagcaga tcctgcaaca
     5401 taaagcgcgc agcacgcgta cctgcatcca ccgccgtcag aataaacaga gcttcaaaca
     5461 gaatggcgaa gtgataccag aacgccacat ccatcatgcc gcccagcgcg ccatgcagaa
     5521 tgtaggccat ccccaccgcc aacgtcggcg caccgcccgc acgggaaatg atcgactgtt
     5581 ctcccacttc gctggcaatc tggttgagcg tatccggagt aatcgcaaag ccccagctac
     5641 tcaccacttg cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca
     5701 tcgggctgtt catggcgaag tacacgcccg gatcgatgat acaggcggaa accagtgcca
     5761 taatcgccac gaaggattcc attaacattc caccgtagcc gataaaacaa gcctgccctt
     5821 cgttcgccag catcttcggc gtggtgccgg aagagatcag cgcatggaag ccagacaccg
     5881 cgccacaggc gatggtgata aacaggaacg ggaacaggtt accggtccat accgggccag
     5941 tgccgtcaac aaatttggtc aacgcaggca tggtcagcgt cgggcgcata atcaaaatgc
     6001 ctaccgccag accaacgatc gtcccgattt tcaggaaggt agagaggtag tcacgcggag
     6061 ccagcagcaa ccataccggc agcaccgccg ccacaaaacc gtaacccacc agcatccagg
     6121 tcagctgcac gccggtaaag tcaaagtacg gtgcccaggt cggactttct gccacccagc
     6181 cgccagaaat aatggcgaaa atcaggaata ccagaccgat gaccgacact tcaccaatac
     6241 gccccggacg cagatagcgc aggtagatcc ccatgaacag cgccagcgga atggtgaacg
     6301 caacggtata cgttccccac gggctatgag tcagggcttt caccacgatc atcgccagta
     6361 ctgcaaggat aatgaccatg atcataaagc aggccaccag cgcgatcacc ccggcggttg
     6421 gccccatctc ttctttgacc agctcaccca gcgaacgacc gtcacggcgc gtagaaacaa
     6481 acagcaccat gaaatcctgc accgcaccgg cgagaaccac cccggcgagt agccagatca
     6541 tccctggcag gtaacccatt tgcgccgcca gtaccggccc aaccaacgga cctgctccgg
     6601 caatggccgc aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc
     6661 cgtcgttatg gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg
     6721 cgatatacag accataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa
     6781 tccacagcgc gttgatctgt tccccacgat ttaaagcaat atatcccaga gcaaatgctc
     6841 ccattacaga gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc
     6901 gtgtgcgtgt ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca
     6961 acacagcaac catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga
     7021 tcactccgtt aaccgagttt tcctgcaatc tcatataatt ctgtcgggtt catcccaaaa
     7081 ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgacgattt
     7141 cccagctctg attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt
     7201 cagacaccgg gcgatgatgt gtggcgttaa gttccgtgcc caccacacac tgcccgtcgc
     7261 gggtcatcat aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc
     7321 catgcagcag gccaaacggc tgatgagtac ggatatcaac cggcatttcg gcttccagca
     7381 catcatcgcc cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg
     7441 tggcgttcag ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt
     7501 tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag
     7561 gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag
     7621 tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag
     7681 cgtgcgttgc atatcggtat cggtggaacc cggcgaaacc acattacagc gcacgccgct
     7741 acccgccagt tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc
     7801 ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc
     7861 cccgccccgc tgacggcgaa actggttcat ggtttgctgg aacaggttaa acgcaccgcc
     7921 gacattaacc gcaaaagtct gctgccagtc ctccttactg agctgatcgg tcgcgcccat
     7981 gcgcaaaatt cccgccgcat tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg
     8041 ctgacatacc tgcgccacct gcgcagcatc ggcaacatcc attacttcgg tcgcaaaggg
     8101 atattgctcc tgagcgaacg cttgatcaaa acctgtaact ttcgctcccg cttcaacaaa
     8161 cgccagcgcc gtggcgtagc cgataccttt ccccgcgccg gttacccaga catttttacc
     8221 gctgaaatcc attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg
     8281 tttttcgcca acatgacaaa gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc
     8341 gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca
     8401 tcggactcgt ccagcaacgg caggatcacc tcacgcagcg ccgctttgct ggcggggata
     8461 ggtgctggca gtaattcttc ggtcatcacc acccggccag aacgtccggc cacatatttc
     8521 agcgacatca aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt
     8581 ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc
     8641 ccggtaataa tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga
     8701 tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcagtcga
     8761 tccaccacct tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac
     8821 agcgcccgat cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg
     8881 tgctgtttgc agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg
     8941 cagttctcgc cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca
     9001 cgttgcggtt caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt
     9061 gcgtaagcct gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga
     9121 cgccagccac tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga
     9181 atccacacac tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa
     9241 acgacgcacc tgcaccgcgc gaagcggctc ttttaccacc agataagcac agcttttttc
     9301 gcccatcagc tcatcttcca tactgaccag tgcggcgtag atcaccgccg gatggcgcag
     9361 taacaggttt tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt
     9421 ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca
     9481 gtaaaaaccg ttggcatcaa aggcgctggc attgtgctgc gggcttttgt aatagccacg
     9541 gaaggtatac ggcccgcgcg tcatcagtcg tccgacttcc ccttgcggca gtggatttcc
     9601 ttcggcatcg gcaacccata cttcgtcatc cggacacatt gggtaaccct gggtatggat
     9661 aattttctcc gcgctatcat caagacgggt atagttcacc agcccttccg ccataccaaa
     9721 cacctgctgc aactggcaac caatctcagc gggaatacgc gccgcaagcg tggcagaaag
     9781 acgtgcgcca ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc
     9841 ggtcagcgcc tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa cgttaatctg
     9901 atgtttttca atcaatggga agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt
     9961 tcctccggca agaaagacgc ccagcgatcc cggcgaactc atggcgtagt tatgagccgc
    10021 cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg
    10081 cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc
    10141 gccggaaagc tggaaataga ccacttcatc agcaggtgac ggcgtagcag taaaaccgtc
    10201 tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gtagctgcac
    10261 cacgcgaatg gaagaatgct ctgcaacaaa tgtgttgagg aaatcatccc cgctaaacag
    10321 cgcatgttgg cgatcggcaa tcagcaatgc gggttcaatc tggctggcat aggcgttcag
    10381 ttcactacgc tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagcgc
    10441 gaaaaaggta atgtaaagtt cagcgacgtt acccagttgt accagcgcgg tttcaccagg
    10501 tttaatgccc tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg
    10561 gtaactcaac tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt
    10621 cagaatgtca gtcagtggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa
    10681 ctcttccggc cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac
    10741 gttcaacatg gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc
    10801 aggcacaatc cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg
    10861 gatggtcacc acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa
    10921 cagttcgcgg tcgaacggct ccagttcagc aataacctgg gtcgcggcct gatgcgggaa
    10981 accgctcagc gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga
    11041 attcgcttta ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg
    11101 tggagaggaa ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg
    11161 agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc
    11221 gcgatcgagc acttcatccg gctgacgacg tgcggaaccg gctaacggaa tggagctaaa
    11281 acgctcaccg tctttacgta gcagcagttc cgggctggcc cccagcagaa cgccaccatc
    11341 tgccagcggg acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa
    11401 tacgccacta tcaatggcgg cgtcagtggt gatatcaatc aaccgtgaca acaccacttt
    11461 gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt
    11521 ttgctccgga attgcctggc gttccaccac gttcagcgac tggctgcggg tgaaacggcg
    11581 tgcggaagct tgtttttcct gacgggagaa cgactgccag gattcaggaa tatacagcga
    11641 cgaaggctga cgtggatcga aaggaatcgc cccgaccatc accggatttt tgatgccctg
    11701 cgctttggca tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc
    11761 cccgttcaca gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta
    11821 cggcgacata aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc
    11881 agccagtgac gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc
    11941 atttatattt tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa
    12001 cgcaatctgc aactcggggt tgcatctggt tgtggttata atgaaaatga gaagcattaa
    12061 cttcattaat aacaggaggc tgatttgtga gactcgcccc gctctaccgc aatgcccttc
    12121 tattaacagg acttttgttt tcaggaatag ccgcagttca ggccgccgac tggccgcgtc
    12181 agattactga cagccgtggc actcataccc tggaaagcca gccgcagcgt attgtttcca
    12241 ccagcgtcac cctgaccggc tcactgctgg cgattgatgc tccggtgatc gccagcggcg
    12301 cgaccacgcc gaataaccgc gtcgcggatg accagggctt tttacgccag tggagcaagg
    12361 tggcgaaaga acgcaaactg caacggctct atatcggcga accgagcacc gaagccgttg
    12421 ccgcgcaaat gccggatctg attttaatta gcgcaaccgg cggggattcg gcgctggcgc
    12481 tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac gacaaaagct
    12541 ggcaggcgct gttaacgcaa cttggcgaaa ttaccgggca tgagaaacaa gcggcagagc
    12601 ggattgcgca gtttgataag caactggcgg cggcgaaaga gcaaatcaaa ttaccgccgc
    12661 agccggtcac tgccattgtc tatactgccg ctgcacacag tgccaatctc tggacgccgg
    12721 aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaag ttacccgcag
    12781 ggttaaatgc cagccaaagc cagggtaaac gccatgacat cattcagctt ggtggggaaa
    12841 atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat cagaaagatg
    12901 ccgatgctat ttatgctaat ccgctgctcg cgcacctgcc tgcagtacaa aacaagcagg
    12961 tttatgcgct gggaaccgag acgttccgtc tggattacta cagcgccatg caagtgctgg
    13021 aacgacttaa ggcgctgttt taagcattaa ccgtcagacg ctgtcacctg cggcggcgtc
    13081 tggcgaaaac gtcgcaactc caccagcacc agcaataaca acacgccgat aatcaataaa
    13141 ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgctcccaa accacccagc
    13201 agcgccgcgc ctatggcatc gcccgtcacg ttttgtgccg tccacaaacc gttaatccgc
    13261 cctaacatcg cttccggggt ttgcgtttgc agcattgtgt attgcagcaa cgagctgacc
    13321 gcactcaacc agccgaacag cgccagacaa accacgccta aaatccacat cggcatcagg
    13381 ccaaacagac caatggcgag gaacgatccc agcgtggaga gcagcatcaa taaccctggt
    13441 cgcgcgctat gcgccagctt cccgctggtt aacgcgccaa tagccgcgcc gagcgggatc
    13501 gccgcataga gaaaaccaat ctgtgccgcc gacatctgcc agttgtcagc cagcgccgga
    13561 tacagtaccc gcaccgcact cgccatcgtc aataaaccac ccagcagcgc aatccctccc
    13621 accagcgggc tggcgagtag aaaacgaaat cctgccagta atgatttcaa cggatgctca
    13681 cgcggctgcg gtggcggtgg cagtgccgga aggcttaaca acggtagcaa ggtaataaac
    13741 gtgcccgccg ccgccagccc gtagttccag gccacaccgc cagtcgccag cagtaaaccg
    13801 ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat cgccccggcc
    13861 tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc cgtaacgcca
    13921 agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa tgacggctcc
    13981 ggcagcagcg cgttcaggca cagtccaatg aagccaatgc cacaggtgcc gcgcgccagc
    14041 aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccgaccat caggccaaca
    14101 aacatcgcgc cgccggtcag cgtcaccgaa agccccacct gccaggtgga atgtgtcatc
    14161 atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat tgagatgaaa
    14221 cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag gttaagcagc
    14281 caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc tattcttagg
    14341 ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca ttatcatgga
    14401 agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg gattgctgtt
    14461 attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac tccccgcttc
    14521 cgtagtgctg gaggccttct ccggcacctg ccagagcgcc gactgcacca tcgtgctcga
    14581 cgcgcggctg ccgcgtaccc ttgccggttt actggcaggc ggcgcgcttg gccttgccgg
    14641 ggcgttaatg caaaccctca cccgaaaccc acttgccgac cccggcttgc ttggcgtgaa
    14701 cgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact cttccgcaca
    14761 ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcgttgattg ttgcctttac
    14821 cggcagccag ggcggtgggc agttaagtcc ggtgcgttta accctggcgg gcgtggcgct
    14881 ggcggcggtg ctggaagggc tgaccagcgg catcgccctg cttaatcctg acgtctacga
    14941 tcaactgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata ccttaaaagt
    15001 ggtgctgatc ccagtgctga tcgccggagc aactgcgcta ttactgagtc gcgcgctgaa
    15061 cagtttaagc cttggcagcg acaccgcgac ggcgctgggc agtcgcgtgg cgcgcacaca
    15121 gttgattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacggcgg tagttggccc
    15181 tattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg gtgccgatca
    15241 tcgctggtcg ctgcccgtca cgctacttgc tacccctgcc ctgctgctgt ttgccgatat
    15301 catcgggcgg gtgattgtgc ccggtgaact gcgcgtttct gtggtcagtg cgtttattgg
    15361 tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat gatttacgtc
    15421 tctcgccgat tactcatcac ctgtttgctg ctgatttccg cctgtgtggt tgcaggtatc
    15481 tggggattac gcagcggtgc cgtcacgctg gaaatctcgc aggtattcgc cgcgctgatg
    15541 ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc acgcgtgctg
    15601 atggcgctgt tgattggcgc ggcactgggc gtcagcggtg cgatttttca gtcgctgatg
    15661 cgtaacccac tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg gagcggcgtg
    15721 ttggtggcga tggtgctgtt tggtcaggat ctgacggcta tcgcgctggc agcaatggtg
    15781 ggcggcatta tcacttcgct gttggtctgg ctgctcgcct ggcgtaacgg tatcgacacc
    15841 tttcggttga ttattatcgg tatcggcgtt cgcgccatgc tggtggcctt taatacctgg
    15901 ctgttgctga aagcgtcttt agaaacggcg ctaacagcag gtttgtggaa tgccggatcg
    15961 ctcaacggcc tgacgtgggc aaaaacctcg ccttccgcac ccatcattat attgatgcta
    16021 attgccgccg ccttactggt acgccggatg cgcttgctgg aaatgggcga tgataccgcg
    16081 tgtgcgctgg gcgtcagcgt cgaacgttcg cgtctgttaa tgatgctggt tgcagtggtg
    16141 cttaccgctg cggcaacagc gcttgccggg ccgatttcct ttattgcttt agtcgcgccg
    16201 cacattgccc gacgcattag cggcaccgct cgctgggggc taacccaggc ggcgctatgc
    16261 ggggcgctgt tactgctggc ggccgatctc tgcgcccaac aactgtttat gccgtatcaa
    16321 cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt tttgttaatt
    16381 caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa cagttaaccc
    16441 tgggatatgg caaatatacc gttgcggaaa atctgactgt agaaatacct gatggtcact
    16501 tcacggcaat tatcgggcca aatggctgcg gtaaatccac gttgctgcgt accttaagcc
    16561 gcctgatgac gcctgctcat gggcatgtct ggctggatgg cgagcacatt caacattacg
    16621 ccagtaaaga agttgcccgg cggattggtc tgttggcgca aaatgccacc acgctgggag
    16681 atatcaccgt gcaggagctg gtggcgcgtg ggcgttatcc acatcaaccg ctgtttaccc
    16741 gttggcgcaa agaagatgaa gaggcggtaa cgaaagcgat gcaggccacg ggaataactc
    16801 atcttgcaga tcaaagcgtg gatacccttt ccggtgggca acgccagcga gcgtggatcg
    16861 cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg acctggctgg
    16921 atatcagtca tcagattgat ttgctggaac tgttaagcga actgaaccgc gagaaaggct
    16981 ataccctggc ggcggtgctg cacgatctta atcaggcctg tcgttacgcc agccatttga
    17041 ttgcattgcg agaagggaaa attgttgctc agggagcgcc gaaggagatt gtcactgctg
    17101 aactgattga gcgcatttat ggtctccgct gcatgatcat tgacgatccg gtagccggaa
    17161 cgccgctcgt ggtgccgctc ggacgaacgg caccttcaac cgcaaatagt taaactaagt
    17221 ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaat aacacgctac
    17281 cacaagccac catcccgccg atcagcgcgg aaaggatcac aataatcgcc ttacccggac
    17341 cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta aaattcacat
    17401 cgttgatatt tgcttttgtt aactgctcaa caagatactg acgattacgt aattcaccgt
    17461 tcagttccgc aacgtcagtg accgcttttt caatttccag tttgcgttca ataccgtctg
    17521 cgccgagaga aatagagaaa tcggggtcat ctttaacggc ctgaccatta ctgtaaacgg
    17581 gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc tgaatgtttg
    17641 catcaagttg atttttcatt ttaatgcggt cctgagccag tttttctttt tcaaactggg
    17701 ttttgatctc cagtttatta cggacgtttt ctatcgactc tttcaccacc aacgcagaga
    17761 tataatcgat atacccgctc aaaacggtct gcgcctcttc actggttggc gcggtaaaac
    17821 ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca ttgtcatcaa
    17881 ccgctttcat tttttcgctc aatgcgacaa ttgcgcgatg caaatccagt tcgtcgattt
    17941 tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct tccagcaagc
    18001 taaccgactg aaacttcttg ataaacaggt taaatgcttc tgtacgatca attttgatat
    18061 ccagatccag cacacgaagc ttagtgaatg ttttctccaa ctcttgccac tgaacaggtt
    18121 ctggaggcgt gacaaccgcc gcgctggtcc atttttgcgg caggatgaaa gagatcagca
    18181 agcctgcaca ggcaaacgca aaaacgaccg ccatgaccgt ttttttggcc cgccataaaa
    18241 ctgagattag attaagtaaa tcaatttcat tattactggg cgacgccaga ggataatcgg
    18301 gaaaatgagc gtcacttccc tgtttaatat tcagtgatga catggggtat aacctgaaaa
    18361 tgagtccaat agggggaaat ctccacggcg ctcactttac cagttggtga gaattatccg
    18421 aagctgaagt ttgtaaattc attccactga atgcaacatt cactctattt aagaaaaata
    18481 accagcaaac ttataaatta aataaatgat aaacccataa ttatttcctg caattatggg
    18541 tttatagata atgttaattt atctgtttag cgttgcgcga ataatcggcc caattttttc
    18601 aaatgcccct ggagagataa tatccacatg cgcacaatcc tgacgataga tatccagctc
    18661 cgcaatccac ggcgaccagg cgcgttcggg gctcatacct tcctgaagcg tacgttcagc
    18721 aacaaacagc gtcgcttttc cgtcaaacgg tacgctatga gcagtcgtca gcaggcgcac
    18781 agcatcagcg tagttgcctt caatggtggt aaacaactcc gttgaagtac ttccctgctg
    18841 tgccgccagg aaggcctcgc gctcgcggtt aatctccgcc agcacttccg ggtccagacc
    18901 attagcttct ttttcctgcc agttttgcgt ttctggcggc caggtatcca gcaagccaag
    18961 aaatgccacc tgttcgccac gggcacgtag tcgcgccgca atgccctgcg ccagtgtacc
    19021 gccaagggaa taccccagca ggtaataagg gccgtgcggt tgttgttcaa gtaacgttgc
    19081 cagatgcgct tcgcagactt catccaggtt cgtcgctgtc tgcatggggc catgagggcg
    19141 cggcgactga atgccgataa tcgaccatag tggatcgaga taacgcgaga gcacgctaaa
    19201 ctgccaggca aaaccggatg ccggatggaa acaaaacagc gtcgggccat taccttcacg
    19261 caacggcaga atggtttcga atcccatgcg ccgggagctg tcctcttcac catcaataat
    19321 cgttgccagt ttggcgacgg ttgacgcgac catcacctgc cccggcgtca cctgacgggc
    19381 aaactgccga cttaactgcg ctgccagttt cattgccagt agcgaatgac cgccaagcgc
    19441 gaagaaatca gcatcggcat cctgcacgtc acaacccagc aacgacgcga atgccgcagc
    19501 gataatcgtt tcactgcccg ctttcggcgc acgccccggc gtttgtgcct taagttcagg
    19561 caacggtaag gctttgcgat ccagcttgcc gttggcgcta agaggtaact gtggaagttg
    19621 cagcagaacg accggcgcca tatgcggcgg caatgtttcg cgaagctgtg cctgtaatgc
    19681 gctggtatcc aacggcagac ctgattgcga caccagatag cccaccaact gacgcgcatc
    19741 accaccggtg gctgccgcct ggttaatcac acaggcgtgg gtaacggctt gttcgacatc
    19801 cggcagcgcc tgcatcacgc gatcgatttc gcccagttcg atacgctgcc cgcgaatttt
    19861 tagctgatcg tcactgcgcc cgaggtactc caccgcgccg ttatccagcc agcgggcaac
    19921 gtctccggta cggtacatcc gttcaccagg gacaaaagga tcggcaatga agcggctggc
    19981 ggtcagatcg ggtcgtccaa gatacccctg cgccagttga ataccggtga gatagagatc
    20041 tcccgccaca cccggcggca ccggatgcat catcgcatcg agaatgcgca agcccgtatt
    20101 ccacaccgga taaccaatcg gcacactgct gccgcgcacc tgtgccagtt cctcgccaaa
    20161 agccggatac caactcacat ctaccgccgc ttccgtcggg ccatatagat tatgcaacgg
    20221 cgcgcccgtt aactgttgcc attcgcggca taaatcagcc ggtaaggcct caccactaca
    20281 gaaaacctgt ttcaacgtcg cgcaattctg gcgagcggtt tgcggcgtca gcgatgcaac
    20341 aaatgccgcc agcatcgacg gcacaaagtg cgtggtcgtt acgccatatt cggcaaagaa
    20401 ttgctgcata gcgagcgggt cgcggtgcgc ttccggttca gccatcacca gtttagcccc
    20461 ggcaataaac ggccagaaaa actcccacac cgagacatca aaactgcacg gcgttttttg
    20521 ggcaacgaca tcttcacctg taagtggata atgattttgc atccacaaca ggcggttaac
    20581 gatagccgtc tgcccgacca ttaccccttt cggcctgccg gtggagccag aggtaaagat
    20641 gatataagcc gtgtgatgcg gttgtgaaag ttgcagcggc gcactgccct gcggtgtaag
    20701 cggggcgtta tagcaaaggt tggttaaatc tggaacatcg gcaaagcgcg gcagttgatc
    20761 gtcggtggtg attaacagcg acggacgcgc atcttccagc atcattttca ggcgatcgtc
    20821 cggataaccg gtatccagcg gtaaccaggc cgcacctgct tcaacaatcg catgtagcgc
    20881 cagggtcaaa aagaccgagc gcggtaatgc caccgccacg ctgtcccctg gtttaacgcc
    20941 gtgctcacgc agcagattcg ccagcgccac cacctgctcg cgcatttccc gatagctgaa
    21001 ctggtaacgc gcatctgcca gcgccggagc atccggtgtt tttgccgctt gttctgccac
    21061 cagcgcgcta agcgtggttt ctggaatctc aacctgagtg gcgttgatct gcgccagctg
    21121 cgcatactca cctggcagca taatatcaac atcgccgcac aacagagccg gatccgcagc
    21181 gaattgggcg atcagcattt tcaggcgttc agcatgctgg attaacgttg gctcatcgta
    21241 atgctgttta ttggcgagga tctcaatact caaatcaccg tgttcatccg ggaacagggc
    21301 cagttcaagg tcattaaccg gaccggttgc cagggtatgg gtttgcgcct gaacaccagg
    21361 aatatccagt tggtaatcaa ataccttgat attgagtacc ggaccaaaca gcggttcatc
    21421 acctgccgct cgcccgctgt cacggacaat ttgttcggca tcgtaacgtt gatgacgacg
    21481 catttttttc agttgtgctg ccagtcgggt tgccagttcc ggcagcgttt cttgtgccgc
    21541 aatgtgaata cccaacggta aaacgttgag cacgggtccg gtagccgtca gcgccgccga
    21601 gcccagtcga cgcataaaga taaatccggc ggcgtagtcc atgcgattgc acaatcgccc
    21661 cagccaaaag gctgccagcg caagggctaa atcggtacgc tgcacacctg aaagttgcgt
    21721 agccagctgg cggaattccc cgtcggtaaa ttccagtttc aggcgtagaa tatctgccga
    21781 agcgctgcgc cccgctaaag gtgccggaga aagtgacgcg ggcggcggca gttgacgacg
    21841 ctgttccgcc cagaatgccg catcacgctg ccaggcttcg ctttcgcggt attgctggta
    21901 ctcttccact acatcagcga aaggcgtaaa cggcgaagca ggcgtttgtt cgccacgcag
    21961 caatgcgcag taaatgttgg cgatctgccg agtaatggcc gggaaactga agccatcgac
    22021 cagcaaatgg tgataacgct gataccagta ccagcggtta tctgccacct gtatcagctg
    22081 gtgaaagacc agtggtttac cgctatcgac gcgcaaatct tgttgcaaat ccgcctgcat
    22141 taatgcctgc gcagtaccgt gcggatcaat attggttcgc aggtcgataa tttctggcag
    22201 ttcgaatatc agcgcatcat cgacccattg ccagacttcg ccgttatctt ccgtaaaacg
    22261 catccgcagc gtatctgctt gcgctaatcc ggcaaccacc gcgcgggcca gtaatggcgc
    22321 atcaacctct ccggttaact caacgtaatg cgccacgctc caggcggagg gtaattctga
    22381 cagtttttct gccatccaga tgccgggctg tgcggcgacc aaaggtaaat gctggctcat
    22441 tgtgcctcct gcaactgggt gaaattcgcc ggtgtcagag tacgccagtg gacttccagc
    22501 cactgctggc aggactcttg tgactgcggc tgacacacaa tgtcccagcc tgccggtaag
    22561 gcgcattgtt gcggccacag actgaattgc ccctgcgcat tgcgcaaaat gtaaaacact
    22621 ccctgtggat catcgaaggg attactgaat gccatattca actcctgtca tggaaaagtg
    22681 gttgccagag gtcgattaac ccctgcatca agccaccgcg ccaacaaagc gcatcatgtc
    22741 cgccgtcaac ctgacgccag aaaatggatt cttttatagg gtgtaattgc gcatacagcg
    22801 cctgattggc ccgcatgatc atcggctcgc gaatacccgc ttccagcaca atgcgcagac
    22861 cttcggcgct aacttcacca gcttttagct tttcaagtaa cacgccctct tgctgcccgc
    22921 cccgatgcgg ccaccagtac gatcctgact ggcttaatac acagccaaag cgttcaggcc
    22981 agtgcagtcc ggcatacagc gcggacagcc caccaaaact ctgcccggcg accacggtgc
    23041 gatcggcgcg atcgctaaaa ggggcaatag ctttcaccag gggtaataac tcttgctgta
    23101 ctgccagcca gaaatccgca ttacacggca gttcgtgggc gcggtgcgtg gtgtcgatag
    23161 cgtcgatcaa cacatacacg gcgggaggaa gttgctgacg atgggtcagc gaaatcagca
    23221 caggccagac gggcatactt tgcgcccaaa attcgccatc gagcaaaact gccagcgggc
    23281 gttcttctgc tgttacatcg ccggtggtaa aaatccatac acgacgtgaa tttttcaacc
    23341 gttcactttt ccagataatt tctttggctg gtatttctgg cgcttgcgga caatcccatc
    23401 ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac agcgtgccct cgcccgcctt
    23461 tccagctttg tggattcagc ggatcggcta ttgcctgggg taatagtttt cgccagcctt
    23521 cacgtaattc gaggcgatcg ggggatggtg cagaaaaaat gtcatcgcgt tcggtgggaa
    23581 taaagcagta gctgccgcgc cagttggcat tgagttgtgt cgtccactgc cagacatcag
    23641 tacctgcaat tcgctgcatc gactggggct ggctgttctg atgatgatcg gtcacaccag
    23701 tgatgtagat ccatacgcgc tttatcgtcg agtattcttc agaaccttgg ggatcacgcc
    23761 accagaaagt gacctcaaac atttcgtcat tcagacgctg ccattccggg ccatgtttcg
    23821 actgccacca gctctcactt cctactttta acgccgtcac accataaccc catgtttact
    23881 gtgcaatttt tcattgattg cagaaatata ttgataatat tattgataac tatttgcatt
    23941 tgcaatagcg taatggcgcg ccgtgggaag cgcggacatt aattaaccaa ctgcactgcg
    24001 tgtctttcag gatcaaaggt tttcgcggta gcgggatgcg tcgtgttgat gacgaccatg
    24061 cccgacagtt gcaattcgtg gcaaaaatgc aggaataaaa caatgaacaa gaagattcat
    24121 tccctggcct tgttggtcaa tctggggatt tatggggtag cgcaggcaca agagccgacc
    24181 gatactcctg tttcacatga cgatactatt gtcgttaccg ccgccgagca gaacttacag
    24241 gcgcctggcg tttcgaccat caccgcagat gaaatccgca aaaaccccgt tgcccgcgat
    24301 gtttcggaga tcatccgtac catgccaggc gttaacctga ccggtaactc caccagtggt
    24361 cagcgaggga ataaccgaca gattgatatt cgcggtatgg gtccggaaaa cacgctgatt
    24421 ttgattgacg gcaagccggt aagcagccgt aactcggtgc gtcagggctg gcgtggcgag
    24481 cgcgataccc gtggtgatac ctcctgggtg ccacctgaaa tgattgaacg tattgaagtt
    24541 ctgcgtggtc cggcagctgc gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc
    24601 attaccaaaa aaggcagcgg cgagtggcac ggctcctggg acgcatattt caatgcgcca
    24661 gaacataaag aggaaggtgc caccaaacgc actaacttta gcctgaccgg tccgctgggc
    24721 gacgaattca gcttccgctt gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat
    24781 atcaaccagg gtcatcagtc cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg
    24841 cgcgaagggg tgatcaataa agatattaat ggcgtggtgc gctgggactt cgcaccattg
    24901 caatcgctgg aactggaagc gggttacagc cgccagggta acctgtatgc gggcgatacc
    24961 cagaacacca actctgacgc ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg
    25021 tatcgccaga actactcgct gacctggaac ggtggctggg ataacggcgt gaccaccagc
    25081 aactgggtgc agtacgaaca cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt
    25141 accgaaggga aatttaacga aaaagcgaca caggatttcg tcgataacga tcttgatgac
    25201 gtgatgctgc atagcgaagt taacctgccg attgatttcc tcgttaacca gacgctgacg
    25261 ctgggtacgg agtggaatca gcagcggatg aaggacttaa gttccaacac ccaggcactg
    25321 accggaacga ataccggtgg cgctattgat ggcgtgagtg ccaccgaccg tagcccgtat
    25381 tcaaaagcag aaattttctc gctgtttgcc gaaaacaaca tggagctgac tgacagcacc
    25441 atcgtaacgc cggggctgcg tttcgatcat cacagtattg tcggcaataa ctggagcccg
    25501 gcgctgaaca tatcgcaagg tttaggcgat gacttcacgc tgaaaatggg cattgcccgc
    25561 gcctataaag cgccgagcct gtaccagact aacccgaact acattctcta cagtaaaggt
    25621 cagggctgct atgccagcgc gggcggctgc tatctgcaag gtaacgatga cctgaaagca
    25681 gaaaccagca tcaacaaaga gattggtctg gagttcaaac gcgacggctg gctggcgggc
    25741 atcacctggt tccgtaacga ttatcgcaat aagattgaag caggctatgt tgctgtaggg
    25801 caaaacgcag tcggcaccga tctctatcag tgggataacg tgccgaaagc ggtggttgaa
    25861 ggtctggaag gatcgttaaa cgtaccggtt agcgaaacgg tgatgtggac taataacatc
    25921 acttatatgc tgaagagtga aaacaaaacc acgggcgacc gtttgtcgat catcccggag
    25981 tatacgttga actcaacgct gagctggcag gcacgagaag atttgtcgat gcaaacgacc
    26041 ttcacctggt acggtaagca gcagccgaag aagtacaact ataaaggtca gccagcggtt
    26101 ggaccggaaa ccaaagaaat tagtccttac agcattgttg ggctgagcgc gacgtgggat
    26161 gtgacgaaga atgtcagtct gaccggcggc gtggacaatc tgttcgacaa acgtttgtgg
    26221 cgtgcgggta atgcccagac cacgggcgat ttggcagggg ccaactatat cgccggtgcc
    26281 ggggcgtata cctataacga gccgggacgt acgtggtata tgagcgtaaa cactcacttc
    26341 tgatgctaac gtcagattgt tgacaaagtg cgcgtcgttc atgccggatg cggcgtgaac
    26401 gccttatccg gcctacaaaa atcttgccaa ttcaatatat tgcaggacca tgtaggcctg
    26461 ataagcgtag cgcatcaggc agttttgcgt ttgtcatcag tctcgaatat ggtcgatatg
    26521 aaaactacgc atacctccct cccctttgcc ggacatacgc tgcattttgt tgagttcgat
    26581 ccggcgaatt tttgtgagca ggatttactc tggctgccgc actacgcaca actgcaacac
    26641 gctggacgta aacgtaaaac agagcattta gccggacgga tcgctgctgt ttatgccttg
    26701 cgggaatatg gctataaatg tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct
    26761 gcggaggtat acggcagtat tagccactgc ggggctacgg cattggccgt ggtttcccgt
    26821 caaccgattg gcgttgatat cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca
    26881 gacaacatta ttacaccagc agaacacgag cgactcgcag actgcggttt agccttttct
    26941 ctggcgctga cactggcatt ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa
    27001 actgatgcag gttttctgga ctatcagata attagctgga ataaacagca ggtcatcatt
    27061 catcgtgaga atgagatgtt tgctgtgcac tggcagataa aagaaaaaat agtcataact
    27121 ctgtgccaac acgattaatt gacaacatct ggtacgattc gcccgcagcc atcactgact
    27181 gcgggcgaaa gtgtaaagca ggtgccttac cactctgacc tgacaaccgg atatgcgggg
    27241 attgctcccc gcacaacggc tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa
    27301 tattacgttc ctttacggtg aactcacaca gcgagtctcc gaccagaagc gtaaatccca
    27361 gcaccgtcaa acacagcact atgactgcca caagggcata tttcgtcagc atgtttatat
    27421 tgcctccatg cgaagaaggg actaccatcc gagttgttgg ggctcggagt ggcagcccca
    27481 ggattgatag aaatatctcc tggggcttca tctttccaga cctcagagtt aacctgaaac
    27541 cggaaagctt caggcacccg ccgttatctt acccttcctt agcaaaatag ctgtttattt
    27601 atacagtgaa tcctgtttcg gacttctgtc ggcaaaactc atcattgctc ttaaaacgaa
    27661 atcgcccgca gccatcactg accgcgggcg aaagtgtaaa gcaggtgcct taccatcctg
    27721 acctgacaac cggatatgcg gggattactc cccgcacaac ggctacttct tcggttcgta
    27781 agcgagaaca gccctgaact caatattacg ttccttcacc gtaaactcac acagcgagtc
    27841 tccgaccaga agcgtaaatc ccggtaccgt caaacacagc actatgactg ccacaagggc
    27901 atatttcgtc agcatgttta tattgcctcc atgcgaagaa ggaactacca tccaaattgt
    27961 ctggtttgga gtggcagccc cagggttgat agaaatatct cctggagctt catctttcca
    28021 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgccgttatc ttacccttcc
    28081 tcaacaaaat agctgtttat ttatacagta aatcctgttt cggacttctc actcctcatt
    28141 taatcaactg aagctgagcc cgttttttct gcatgcctca atcgtcaaaa ttcttcgcca
    28201 ccagccagcc gtgccataac ttgtcacatt gcaaaagtag ccaaattgct tcagtcacca
    28261 tcaccaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat
    28321 actgaataga aaactccaac ataagagaaa cctatgccat tacccgattt tcatgtttct
    28381 gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac
    28441 ttaagccagg actcttcaat gctgattgac gcggttaaaa ataagatcac ggccggagag
    28501 gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc
    28561 aaccaggctg ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac
    28621 catcatctgg aaatttgcgg cggtggcacg cacccgtttc agaaatggca gcgtcaggag
    28681 gtatgcgaca acgaacgcta tcaacgaacg ctggaaaact ttggctatct catccagcag
    28741 gcgaccgttt ttggtcagca tgtccatgtt ggctgtgcca gtggcgatga cgccatttat
    28801 ttgctgcacg gcttgtcacg gtttgtgccg cactttatcg ccctttccgc cgcgtcgcca
    28861 tatatgcagg gaacggatac gcgttttgcc tcctcacgac cgaatatttt ttccgccttt
    28921 cctgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc
    28981 tgtctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc
    29041 agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac
    29101 gcggtaaata tggcgggatt aattcaggcc accgcccact ggttactgac agaacgcccg
    29161 ttcaaacata aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgccgc
    29221 tatgggctgg aaggcgtcat taccgatccg tacactggcg atcgtcgacc actaacggaa
    29281 gacaccttgc gattgctgga aaaaatcgcc ccttctgcac ataaaattgg tgcatcgagc
    29341 gcgattgagg ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc
    29401 gatttcgtcg ccgatggcgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg
    29461 gccggtgact aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac
    29521 ttacatttaa caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt
    29581 tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg cttacgctgg
    29641 cacaaaaact ggtgaccacg ttccatctga tggatcttag tcagctttac actttattgt
    29701 tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc
    29761 gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt
    29821 gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg
    29881 aaacgcctgg cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc
    29941 gatgttttta aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc
    30001 tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc
    30061 attaagccag ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa
    30121 atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt
    30181 ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt
    30241 tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca
    30301 catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt
    30361 gatgccagca aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac
    30421 agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt
    30481 aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg taaaatactt
    30541 gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag
    30601 ctggttgttg accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg
    30661 aacgataaag gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca
    30721 gagtggatgg caaaacaggt ttatctcaac gtcggtaatt tcctgctcgg cgtggcggct
    30781 ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt
    30841 ggtctgaaag agaaaggcta caccagtctg gtggtagttc cggtgggtca tcacagcgtt
    30901 gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacattac cttaaccgaa
    30961 gtgtaattct cccttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg
    31021 cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg
    31081 atttaatatc ccatgttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg
    31141 tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg
    31201 tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca
    31261 ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata
    31321 ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg
    31381 cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgctgctg gatgttattt
    31441 tgaatctggc gcagaaattc ccggcagcat ctcagttacc gctgaaaggg atatttcagg
    31501 ggattaaact gatcggcgcg attctggtcg gcattttgat gatctcgctg ctgattggtc
    31561 agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat
    31621 ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga
    31681 aactgggcga ctggctggag atgccgaaat acggcgcgga tggcgcggtg atcgatattg
    31741 ggttaaccac cgtcaaggtg cgtaactggg acaataccat taccactatt cccacctggt
    31801 ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta
    31861 ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc
    31921 aacgtctgaa taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta
    31981 atgagtggaa tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca
    32041 atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta
    32101 aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga
    32161 tctatgcgtt taccaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat
    32221 tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg
    32281 gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat
    32341 cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg
    32401 cagcgtttta aatgctacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa
    32461 gcgcatatca tccatcgtgc acatcagcag caaaatcatg gcgaggaagg cgatgcaaag
    32521 atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat
    32581 cgctgcacga aaacgcagat gcgccagaca gatcataatc cagttcaaca gcagcgttgc
    32641 taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac
    32701 caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacgc cgcgacggct
    32761 aacacgggta aggaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct
    32821 gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag
    32881 cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt
    32941 ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgaac cgatgtaaaa
    33001 cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg
    33061 cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc
    33121 cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt
    33181 gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat
    33241 cataccgatg attgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa
    33301 gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca
    33361 cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat
    33421 tcccaccagc acgaacatca cccagtagtt ccagccggag aggaacccgg cgaacggtcc
    33481 ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc
    33541 gccgagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac
    33601 agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc
    33661 gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc
    33721 ttgattcgac gcagtatctt ccgatacgtt tgacgcgttt ttcacgcctt tcccctgtgt
    33781 gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc
    33841 acacggttaa accgggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc
    33901 agcttatacg ctgccgggat aataaacagc gacagcaaag gcgcggtgat catgccgcca
    33961 atcatcggcg cggcaatccg gctcatcacc tctgatcctg cccccgttcc ccacagaatc
    34021 ggcagcagac cagcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc
    34081 gcgccgtgat gtaacgcctc atccagcttc tgctcgctga atgtttgcgg attattcaac
    34141 gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg
    34201 gcgaccccgg caagggcaat aaaaccggtg cccgtcgcca cagaaagatg aaagcccatc
    34261 caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac
    34321 gcttcgccca cccgacggaa cgccagatac aacagcacga agatgatcat caacgtcatc
    34381 ggcaccatga gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat
    34441 gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga
    34501 accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca
    34561 ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg
    34621 gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg
    34681 cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg
    34741 ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata
    34801 ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc
    34861 cgctcggcaa gcgcagaagc cacgcctggc accgttcgtg ccacctcttc aatttgctcc
    34921 gccatcgcgt cgatatccgc cagcacagtg ccggaaactt ttatgccgat agggctttta
    34981 atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc
    35041 cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccattgt catgcctggc
    35101 cgccactgct cctgcggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa
    35161 tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc
    35221 ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc
    35281 agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca
    35341 actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt
    35401 ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg
    35461 agcgggttac tgctttccgg cggaattttg ccacggatcc agtaacccat caggataggg
    35521 atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccagc
    35581 gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacagcgtg
    35641 ataatcagca gactgataaa cagcgccggc cccacttcaa cagatgcatt ggtaatcacc
    35701 tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc
    35761 cgtttatgtg cattctcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc
    35821 gcaatgccac ccaacgacat aatattggca ttcagcccct ggaagtgcat gacaataaaa
    35881 gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag
    35941 agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg
    36001 ttgtcgatgg cccggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct
    36061 tccggcagac tgcttttcag cgtttccagt ttgtccttca cggcggcgat cacttcacgg
    36121 gcgtttttgc cggagcgtag gatcaccacc ccgcccacaa cttcgccgtt cagttcggca
    36181 atgccccggc gcatctccgg gccaacctgg atcttcgcaa catcacgcag ataaacaggc
    36241 acgccatttt cactggcttt taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag
    36301 ccgctggcgc gcaccatata ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc
    36361 tggtttgaag catccagcgc gctttttact tcggcgagac tgatgccata ctgcgccagg
    36421 cgctgaggat cgataaccac ctgatactct ttcaccacgc cgcccaccga cgccacttcc
    36481 gcaacgtcag ggatggtttt tagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa
    36541 tcggccagat cgtgcttacc gctgcgatcc accagtgcat attcatagat ccagccaaca
    36601 cccgtggcat ctggtcccag ctcggcgctg actcccgcag gcagcttacc ctgtacctgg
    36661 ttgaggtact ccagcacccg cgaacgcgcc cagtacggat cggtaccatc ttcgaaaatg
    36721 acatacacat aagagtcgcc aaactgcgag aaaccacgca cagtcttcgc gccaggcacc
    36781 gacaacatgg tggtagttag cggataagtc acctgatttt caacgatttg cggtgcctga
    36841 ccgggatagc tggttttaat aatcacctgc acatcggaga gatccggcaa cgcatccact
    36901 ggcgtgttaa tgatggtcca ggtgccccag atactcagaa ataacgcgcc catcagcacc
    36961 aaaaaacggt tcgccaccga gcgacgaata atccattcaa tcattggtta ttccctcaat
    37021 gcgcatgggt agcactttca gagcgcatcc gctccagtgc gccagaaata ttggcttcgg
    37081 aatcaatcag gaacaggccg ctggaaacca ccttttcacc ttccgccaga ccagagcgta
    37141 atgcggtgac accttgcgat gcctggaaaa cggcaacgcg ttttggtaca aagcgcccgt
    37201 cggcatcaac ggtaatcacc cgctgttcgc tgccggtatc aatcagcgcc tgtgacggaa
    37261 tgagcagcat cggttcgctg gcggtattga gttgcaacca ggcgttcatt cccggtttta
    37321 gcgcctcgtc ggcgttgtcg acttccagac gcagctgcag cgtgcgggtc gcggcatcca
    37381 cgccaggtag tagcgtccat ttgcggatgg tgagtgtttt atcaggtcgc gccggaacgg
    37441 tgagcgtaaa ctgcgaggca tctttcacca gccaggcgat ggactccggg atcgcagcag
    37501 tgacccacac cgggtccata ccctgaattt tcgctaccac gttatctttg gcgatattca
    37561 ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat gggtgctttg agcgtaaagc
    37621 gagtctggat tttttgcgtg gcgatcaggc ggcgaatatc cgcctccggc attcccgcca
    37681 gtcgcagccg ctcaagaatg ccttcggtct gggtcgccgt accgccggtt tcgcgcagca
    37741 gtaaatactc actctgcgct tccacccagt cgggaatggt caggtcgaga agcggcgcgc
    37801 ccttttgtac tttatcgccc acggtaagcg gatacacctt gtcgataaac ccggcggcgc
    37861 gagcctgcac aatggcatac tgatactcgt tgtaactgac attcgccggg aaactctggg
    37921 caaaagtcag cggcccgcgc gtgacggtag ccgttttcac ccccagattc tgcgtctgag
    37981 tcgggtcaat gcgcacacca gacgcagaac tctcttcatc ggcatatttc ggcaccagat
    38041 ccatatccat aaacggcgat ttacctggtt tatcgaaccg cgtattggga tacatcgggt
    38101 cgtaccagaa taagatttta cgttctgcgg tcgacgtttt ttctgcaggc ggttccgcct
    38161 ttgcaaacca ggtaaaacct gccgcagaaa taataccgcc cgcgatcatg ctgccgataa
    38221 taagcgcgat ttttttcatc tcattaaacc tgggttactg gctgacttta atatcctgta
    38281 ataaagaaag gttgccctgc tggacaaaat taaacgccac tttgtcgccg gttttaattc
    38341 cactcatttt cgtctgcggg gtgatggtaa agcgcatggt catctccggc cagttcacgg
    38401 cagcaatcgg atcgtgatgg atggtgattt ttttgctttc cagatcaaaa ccttttacca
    38461 cgccggtggc actaatgacc tgtggttgtg cttcgctcat ggtttcatga tgatgttcgt
    38521 tagcctgggc attaaagcca ataacggtaa acagactgaa cattgcgact tgcagtgctt
    38581 ttttcattta ttttctcctg gagttaaaaa ttattgctgc caaccgccac caagtgcggt
    38641 atataaagaa atttcgttga cctgacgggc ataattcagg tcaagtacgg tttgtcgggt
    38701 tgcaaataaa gaacgctcgg catccagcac ttccagataa cttactgcgc cgtgctgata
    38761 taatgcccgc gcccgttgca aagtaatttg cagcgatgcc agataacgct gctgggcgct
    38821 aatttgatcg ttcaggcttt gacgtaatgc cagcgcatct gccacttctt taaaggcgtt
    38881 ctggattttc tgttcataat tcactaccga ctgctgctgg cgaatttcgg cgatatccag
    38941 attggcctgg ttgcgtccgg cattaaaaat ggggatctca attttaggaa taaaattcca
    39001 catcccgctg ctggcgttaa ataatgacga cagatcgctg ctggcggttg atattccgct
    39061 ggtcaggctg atagacggga aaaatgccgc gcgtgcagca ccaatattgg cattagccgc
    39121 cattaacgca tgttcagctt ccataatatc cgggcgctgc aataagattt gcgacgacaa
    39181 gcccgccggt aatttaacgc tttgcaggct gtcgctgttt actgtctgcg cttgcggcag
    39241 cttgccgtag cttcccagta acagttgcaa tgcattattc gcctgcgcca gttccccctg
    39301 acgtttagcg atgtcgctgc gggtactttc aatcaccccg cgagcctgtt ccagcgccag
    39361 aacattactg ctacctgtca acagttgttt ttcgacaaac gcatatgact gctgataatt
    39421 acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc agctgctgat tgaaatagct
    39481 ttgcgcgaca ttagaaacca gcagaatatg caccgcgcgc tgagcttcct cagtggctaa
    39541 ataattttgt cgctcggctt cgctcatgtt ctttaagcgc ccgaaaaaat cgaggtcaaa
    39601 gctggcgtta aggccagtag aaaactcccg cgtcgtggct gaatcgcctt taagattgcc
    39661 gctccagctg ccgctgccct caccattgag ctgtgggtag cggtcggcat cagtcagacg
    39721 atattgcgcc cgtgcctcct gcactttcag cgtcgccatg cgcaaatccc ggttattctc
    39781 cagcgcctcg ctaatcagcg tcttcacctg attatcaaca aaaaaggtgc gccagcccgc
    39841 gttctgatag ttatctgctg cgttaaccag gccgttctgg ctgagtgaga actgctgcgg
    39901 tacgggcatt gccgggcgct gataatccgg tgccagtgaa caaccggtta gcgcaagggc
    39961 cacacaaaat ggcagaagtt tacaaggaga cataggctca taatttctgg tgattttata
    40021 ccgccaactt tactcgccag gctctgattt tccggtgaca ggaaaatgac aaaattgtca
    40081 ttttgccaat aagcgattgc catctgatcc cgctactcta gaattgcccg ggcaacatgc
    40141 ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga aaaccggaga atacttgacc
    40201 aaagggttaa ccgaagccgg ttttgtggtc gatttggccg acaacgggct gaatggctac
    40261 catctggcga tgaccggaga ttatgacctg ataatcctcg atattatgct gccggacgtg
    40321 aacggctggg atatcgtgcg catgttgcgc tccgccaata aagggatgcc gattctgttg
    40381 cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc tggagttggg ggccgatgac
    40441 tatctggtga agccgttcgc gtttgctgaa ctgctggcgc gggtgcgcac tctcctgcgg
    40501 cgcggggcgg cggtgattat cgaaagtcag tttcaggttg ccgatttgat ggtcgatctc
    40561 gtcagccgca aagtcacccg cagcggcacg cgcatcactc tgaccagtaa agagtttact
    40621 ctgctggagt tttttctccg ccatcagggc gaagtgctgc cccgctcgct tatcgcctcg
    40681 caggtatggg acatgaattt tgacagcgac actaacgcca ttgatgtggc ggtgaagcgg
    40741 ctacgcggca aaatcgacaa cgactttgag ccgaagctga ttcaaaccgt gcgcggcgtg
    40801 ggttacatgc ttgaggtgcc ggatggtcag taagccattt cagcgcccgt tttcgctggc
    40861 aacccgcctg acctttttta tcagcctggc caccatcgcg gcgtttttcg cctttgcatg
    40921 gatcatgatc cactcagtaa aagtgcattt tgccgagcag gatattaatg atttaaaaga
    40981 gattagtgcc acccttgaac gcgtactaaa tcaccctgac gaaacgcaag cccgacgctt
    41041 aatgacgctg gaagatatcg tcagtggtta ttccaatgtg ttgatttccc tggcagatag
    41101 ccacggtaaa acggtgtatc actcccccgg tgcgcctgat atccgcgagt ttgcgcgcga
    41161 tgccataccc gataaagacg cgcggggcgg cgaggtgttt ctcctctccg gcccgacgat
    41221 gatgatgcca ggccacggtc acgggcatat ggaacacagc aactggcgga tgatcagttt
    41281 gccagttggc ccactggttg acggcaaacc gatttatacg ctctacatcg cgctttcgat
    41341 cgattttcat cttcattaca taaatgattt gatgaataaa cttattatga ccgcatcggt
    41401 catcagcatc ctgatcgtct ttatcgtgct attggcggtt cataaaggtc acgcgccgat
    41461 ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa gatctcgacg tccgccttga
    41521 cccgcagacc gtgccgattg agctggaaca gctggtactg tcgttcaacc atatgatcga
    41581 gcgtattgag gatgtcttta cccgccagtc caatttctca gcggatatcg cccacgaaat
    41641 tcgcacacca attactaatc tcataacgca aacggaaatc gccctcagcc agtcgcgcag
    41701 ccagaaggag ctggaagatg tgctctactc taatctcgaa gagctgacgc gaatggcgaa
    41761 aatggtcagc gatatgctgt ttctcgccca ggccgataac aaccagttaa tccccgaaaa
    41821 gaaaatgctc aacctggcgg atgaagtcgg caaagtgttc gattttttcg aggctttagc
    41881 ggaagatcgc ggcgtggagc tgcagtttgt tggcgacgag tgccaggttg cgggcgatcc
    41941 gctgatgctg cgtcgggcgt taagcaacct gctttcgaac gccctgcgtt atacgccacc
    42001 cggagaggct attgtagtgc gctgccagac ggtcgatcat ctggtgcaag ttatcgtcga
    42061 aaaccccggt acgcccattg cgcccgagca cttaccgcga ttgtttgacc gtttctaccg
    42121 cgttgacccc tcccgccaac gaaaaggcga aggtagcggc attgggctgg cgatagtgaa
    42181 atcaattgtt gtcgcgcata aaggcacggt tgcggttacg tcaaatgcgc gggggacaag
    42241 gtttgtgatt gtgttgccgg agagggggtg attattaagt tcaaattcag ggcgagggtt
    42301 tacggcgttt ttcgctaaat ttccatcatt ataaacaagc cacctatc
//
LOCUS       DBFIFS010000015        42254 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.015, whole genome shotgun sequence.
ACCESSION   DBFIFS010000015 DBFIFS010000000
VERSION     DBFIFS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.015"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            182..808
                     /gene="ydhY"
                     /locus_tag="N7A07_000777"
     CDS             182..808
                     /gene="ydhY"
                     /locus_tag="N7A07_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU2475502.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /gene="ydhV"
                     /locus_tag="N7A07_000778"
     CDS             829..2931
                     /gene="ydhV"
                     /locus_tag="N7A07_000778"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU2475503.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2944..3582
                     /gene="ydhW"
                     /locus_tag="N7A07_000779"
     CDS             2944..3582
                     /gene="ydhW"
                     /locus_tag="N7A07_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU2475504.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3646..4314
                     /gene="ydhX"
                     /locus_tag="N7A07_000780"
     CDS             3646..4314
                     /gene="ydhX"
                     /locus_tag="N7A07_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU2475505.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICACACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4311..5096
                     /gene="phsC"
                     /locus_tag="N7A07_000781"
     CDS             4311..5096
                     /gene="phsC"
                     /locus_tag="N7A07_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU2475506.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5100..5912
                     /gene="ydhT"
                     /locus_tag="N7A07_000782"
     CDS             5100..5912
                     /gene="ydhT"
                     /locus_tag="N7A07_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU2475507.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5924..7528)
                     /gene="ydhS"
                     /locus_tag="N7A07_000783"
     CDS             complement(5924..7528)
                     /gene="ydhS"
                     /locus_tag="N7A07_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU2475508.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTLLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7654..7959)
                     /gene="ydhR"
                     /locus_tag="N7A07_000784"
     CDS             complement(7654..7959)
                     /gene="ydhR"
                     /locus_tag="N7A07_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU2475509.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8067..8143)
                     /locus_tag="N7A07_000785"
     tRNA            complement(8067..8143)
                     /locus_tag="N7A07_000785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8107..8109),aa:Val,seq:gac)
     gene            complement(8148..8224)
                     /locus_tag="N7A07_000786"
     tRNA            complement(8148..8224)
                     /locus_tag="N7A07_000786"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8188..8190),aa:Val,seq:gac)
     gene            8532..9788
                     /gene="ydhQ"
                     /locus_tag="N7A07_000787"
     CDS             8532..9788
                     /gene="ydhQ"
                     /locus_tag="N7A07_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU2475510.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTLTVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="N7A07_000788"
     CDS             complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="N7A07_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU2475511.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11417..12058
                     /gene="ribE"
                     /locus_tag="N7A07_000789"
     CDS             11417..12058
                     /gene="ribE"
                     /locus_tag="N7A07_000789"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU2475512.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="N7A07_000790"
     CDS             complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="N7A07_000790"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU2475513.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13537..14748)
                     /gene="punC"
                     /locus_tag="N7A07_000791"
     CDS             complement(13537..14748)
                     /gene="punC"
                     /locus_tag="N7A07_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU2475514.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14861..15793
                     /gene="punR"
                     /locus_tag="N7A07_000792"
     CDS             14861..15793
                     /gene="punR"
                     /locus_tag="N7A07_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU2475515.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15790..16815)
                     /gene="purR"
                     /locus_tag="N7A07_000793"
     CDS             complement(15790..16815)
                     /gene="purR"
                     /locus_tag="N7A07_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU2475516.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17114..17203
                     /gene="cydH"
                     /locus_tag="N7A07_000794"
                     /gene_synonym="ynhF"
     CDS             17114..17203
                     /gene="cydH"
                     /locus_tag="N7A07_000794"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU2475517.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17369..18538
                     /gene="ydhP"
                     /locus_tag="N7A07_000795"
     CDS             17369..18538
                     /gene="ydhP"
                     /locus_tag="N7A07_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2475518.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18684..19265)
                     /gene="sodB"
                     /locus_tag="N7A07_000796"
     CDS             complement(18684..19265)
                     /gene="sodB"
                     /locus_tag="N7A07_000796"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU2475519.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19393..20208)
                     /gene="mepH"
                     /locus_tag="N7A07_000797"
     CDS             complement(19393..20208)
                     /gene="mepH"
                     /locus_tag="N7A07_000797"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU2475520.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20543..20890
                     /gene="grxD"
                     /locus_tag="N7A07_000798"
     CDS             20543..20890
                     /gene="grxD"
                     /locus_tag="N7A07_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU2475521.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20941..25557)
                     /gene="lhr"
                     /locus_tag="N7A07_000799"
     CDS             complement(20941..25557)
                     /gene="lhr"
                     /locus_tag="N7A07_000799"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU2475522.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25650..26297)
                     /gene="rnt"
                     /locus_tag="N7A07_000800"
     CDS             complement(25650..26297)
                     /gene="rnt"
                     /locus_tag="N7A07_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU2475523.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26400..26807)
                     /gene="gloA"
                     /locus_tag="N7A07_000801"
     CDS             complement(26400..26807)
                     /gene="gloA"
                     /locus_tag="N7A07_000801"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU2475524.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26888..27985)
                     /gene="nemA"
                     /locus_tag="N7A07_000802"
     CDS             complement(26888..27985)
                     /gene="nemA"
                     /locus_tag="N7A07_000802"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU2475525.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28022..28621)
                     /gene="nemR"
                     /locus_tag="N7A07_000803"
     CDS             complement(28022..28621)
                     /gene="nemR"
                     /locus_tag="N7A07_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU2475526.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV"
     gene            28724..28963
                     /gene="ydhL"
                     /locus_tag="N7A07_000804"
     CDS             28724..28963
                     /gene="ydhL"
                     /locus_tag="N7A07_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU2475527.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29012..29908
                     /gene="ydhF"
                     /locus_tag="N7A07_000805"
                     /pseudo
     CDS             29012..29908
                     /gene="ydhF"
                     /locus_tag="N7A07_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            29989..30510
                     /gene="sodC"
                     /locus_tag="N7A07_000806"
     CDS             29989..30510
                     /gene="sodC"
                     /locus_tag="N7A07_000806"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU2475528.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30511..32523)
                     /gene="ydhK"
                     /locus_tag="N7A07_000807"
     CDS             complement(30511..32523)
                     /gene="ydhK"
                     /locus_tag="N7A07_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU2475529.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHTSLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32523..33380)
                     /gene="ydhJ"
                     /locus_tag="N7A07_000808"
     CDS             complement(32523..33380)
                     /gene="ydhJ"
                     /locus_tag="N7A07_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU2475530.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33383..33619)
                     /gene="ydhI"
                     /locus_tag="N7A07_000809"
     CDS             complement(33383..33619)
                     /gene="ydhI"
                     /locus_tag="N7A07_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU2475531.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33820..34254
                     /gene="slyA"
                     /locus_tag="N7A07_000810"
     CDS             33820..34254
                     /gene="slyA"
                     /locus_tag="N7A07_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU2475532.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34301..34768)
                     /gene="slyB"
                     /locus_tag="N7A07_000811"
     CDS             complement(34301..34768)
                     /gene="slyB"
                     /locus_tag="N7A07_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU2475533.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35041..36150
                     /gene="anmK"
                     /locus_tag="N7A07_000812"
     CDS             35041..36150
                     /gene="anmK"
                     /locus_tag="N7A07_000812"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU2475534.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36248..36577
                     /gene="mliC"
                     /locus_tag="N7A07_000813"
     CDS             36248..36577
                     /gene="mliC"
                     /locus_tag="N7A07_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU2475535.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36636..37292
                     /gene="pdxH"
                     /locus_tag="N7A07_000814"
     CDS             36636..37292
                     /gene="pdxH"
                     /locus_tag="N7A07_000814"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU2475536.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37421..38695
                     /gene="tyrS"
                     /locus_tag="N7A07_000815"
     CDS             37421..38695
                     /gene="tyrS"
                     /locus_tag="N7A07_000815"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU2475537.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38757..39617
                     /gene="pdxY"
                     /locus_tag="N7A07_000816"
     CDS             38757..39617
                     /gene="pdxY"
                     /locus_tag="N7A07_000816"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HGU2475538.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39661..40266)
                     /gene="gstA"
                     /locus_tag="N7A07_000817"
     CDS             complement(39661..40266)
                     /gene="gstA"
                     /locus_tag="N7A07_000817"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU2475539.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40372..41874)
                     /gene="dtpA"
                     /locus_tag="N7A07_000818"
     CDS             complement(40372..41874)
                     /gene="dtpA"
                     /locus_tag="N7A07_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU2475540.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQNGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10639 a        10402 c        11037 g        10176 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat attttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgttgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat gactaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagtcgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gcagatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgcttgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaatga ttacatgatg
     1501 actgaactta tcggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgctggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggcggtc caattgaaac gggtgaaatt ccgccaggca atcagaatac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcagcag agaaatacac agtaaaaatg
     1741 agcggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag caaactttgt ccataccacc
     1861 atcttcccga acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac
     1921 ctggttggtc tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagttgcat
     1981 cgcgacttta cttattgtta cagcaaaggt gtgttcaagc gtgttctgcc tgctgaagag
     2041 tatgcagaaa ttcgctggga tcaactggaa gcgggagacg ttaacttcat taaagatttt
     2101 tactaccgtc tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc
     2161 atcgcagaat gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg
     2221 tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt
     2281 gttaactgta tgttcaaccg tgactgcatg acgcataccc atatcaactt tattggttcc
     2341 ggcttgccat tgaaactgca acgtgaagtg gcgaaagaac tttttggttc tgaagatgct
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgctaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcactggcga agagatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacat tgcatcgtgc ctacacggtg aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact
     2761 gaaggtactg acaaaatgga tcgtgatgat atgcatgcct cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatgggt
     2941 aagatgaacc atcaggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag
     3001 cggcaatggt tgcaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgtggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcagttgca gaagaggaac tgtcagtttt gctggaaaac
     3181 atcaagcaga acggagacta cgcggatatc gcttgtatga cgggaagtca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt
     3361 ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa
     3421 gccgcgatcg ccgcaatgga tgttgcacct gaatatgccg atatacggca ggtagagtca
     3481 tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc
     3541 ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca
     3601 tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga
     3661 cgcaaatttg ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg
     3721 ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg
     3781 tgcaatggct gtaatatttg cgcctgcgca tgccgtaaaa ctaaccacgc ccccgctcag
     3841 ggaagccgtt tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat
     3901 cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg
     3961 acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc
     4021 ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact
     4081 aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc
     4141 atttgcgtca gcgcttgccc ggaacacgca ttgatctttg gtcgcgaaga tagccctgaa
     4201 attcaggcgt ggttgcagga taataaatac tatcaatatc agctacctgg tgccgggaaa
     4261 ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt
     4321 cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg
     4381 aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc
     4441 tggggctgca cgccttgctt cgtgctcgtg gcgtgaagaa atcagccacc gatcatggtg
     4501 agaggattta tctttacagc aaagcggtca gattatggca ctggtcgaat gcgttactct
     4561 ttgtattgtt gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg
     4621 ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactggca tgctggctcg
     4681 gctttgttct gatcaatggc gttgggggta atggccacca ctatcgcatt cgtcgtcagg
     4741 gttggctgga gcgggcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg
     4801 aagaacatcc tttcccggca acaacccagt ctaaatttaa tcccttacag caggttgcct
     4861 atgttggtgt catgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct
     4921 atccgcaagc cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt
     4981 ttgctctggc atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg
     5041 ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa
     5101 tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt
     5161 tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg
     5221 aaggatatac ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg
     5281 atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag
     5341 agagagcatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg
     5401 aggataacgc cagaatcgca accgctgggt atgctgcaca aattgccagt atgggttata
     5461 gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt gagcgcgcac
     5521 gggttgctgt aaccggcaat tcttcgcgga taagcagcgc tggagatagc agtcggattg
     5581 cgaataccgg aatgcgagta cgcgtttgta cattgggtaa acggtgtcat gtcgccagta
     5641 atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg
     5701 acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg
     5761 actcaattat tctcgggcca gggggcagcg ccgcactggc atatcatgac ggcgaaagag
     5821 ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg agccggagtc cgctatcgtc
     5881 ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac
     5941 gccgacgagc acgggatgca ggctttacga ccgcccgagc catcgcctca ccaatttctg
     6001 cacatgccgt aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga
     6061 acgctacgcg cccacgaata tcttcgggtt gctgtaacgt ataatcttca ccaacatcag
     6121 ggatttcatc ccctgttttc tgtaattgtt cgcgtagtcc agggaaagga atatctttca
     6181 ctttaagcgg acgctgcccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt
     6241 ccgttttcag gacggtgcgc gactcgttaa tttccatttt gtagtcttca ccgagagcga
     6301 gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga
     6361 ttgccgcata attatcgatg aaaacccggg caaggccttt actgaaccgt ttatggtcgt
     6421 gttcattcag atgtggaaca atttcctgta cggcttcatg caggcgcaga atgacataac
     6481 gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg
     6541 cccagtcaaa ttggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga
     6601 cattcaggct ctctaaggct atgcgttgac tccagtctgg atcagcaaac ttgatttcct
     6661 ctactatcaa tctaaatacc cgatccaaaa ggccatattc gcctttttga atctcagcat
     6721 ttaatgcctg atcagtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg
     6781 cttcgggtaa aatacccgtg cgcgacatta acgtaatatt tagcttttca cttgcgttat
     6841 cgcggtgaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca
     6901 ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat
     6961 cgacctttgc ttccatcaag cctgaccacg ggcttggaaa atacgttcgg gttgcttctt
     7021 cttcatcagg ccagacgtga cccgtggcga tcaccgctaa atcaaacgtc tcgctgggta
     7081 aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc
     7141 atgattcata aacagccact gcaaattttt gctttcgtgc ctggtctact aatcgtaaaa
     7201 attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg
     7261 tttctttttt aacgccataa cgctggagat gactggcttc ttgcttttgt agccattcga
     7321 gatacgtaca ataaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg
     7381 agttttcctc atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag
     7441 aaatagaaag tagagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc
     7501 cggcacccac aattgcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt
     7561 agtgttatca gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg
     7621 aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg
     7681 actcagtggc tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagtttttt
     7741 cagttgggca gtgtgttttt ccagataggc aagggcgctt ttttcatcag tgaaaaggta
     7801 gatcccaccg gcttcgtggt tcttttcact ttctgtccat accttccaca gaaaaccagg
     7861 ttcctgatta atcgactctg caagtggttt aagctgctca gccattgcgt cgccaaatgg
     7921 gccattaaaa gcaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac
     7981 gcatacgaag acagtgtaaa tcttttaacg tattggatac agagaagaag cagagggaaa
     8041 aaggagaaga ccagattaac aaaatctggt gcgtccgagt ggactcgaac caccgacccc
     8101 caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat
     8161 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga
     8221 acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag
     8281 aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc
     8341 tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa
     8401 cccaataatt cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg
     8461 tggataaatc ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa
     8521 aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg
     8581 ttaagaccgg cgaggtcatt ggcgcgacgc aacttactga aggcgagttg attgttgaag
     8641 ctggcggaag agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtggcaaccg
     8701 gtgggatcgc caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg
     8761 ccattgccac agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta
     8821 cggttaatgg ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt
     8881 tgttgctgga aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatca
     8941 ttctcgatca agagggcggc ctgttggtta atgggacaac ctcagcggtc gtggtagatg
     9001 aaggtggtga attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg
     9061 gcggcgtttt tatgctggcg gggaaagcca atgatacgtt gcttgctgat ggcaccatga
     9121 ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggagccatc tatcgtctgg
     9181 ggacggatgg tcttcagctc tacagttccg gtaagacgca aaacctgtcc gttaatgtgg
     9241 gtggtcgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag
     9301 tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac
     9361 cggttgaact gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg
     9421 gcgcagatct gcaacaatca acgcttactg tacagcaggg cggtgtattg attctcgacg
     9481 gcagtacggt aaaaggtgac agtgtcactt tcagtattgg taacatcaat ctgaatggcg
     9541 gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca gctgaaagtg aaacgcctgc
     9601 gcggagaggg agcgatttgc ctgcaaacca gtgcgaaaga aatttcacct gacttcatca
     9661 atgtgaaagg ggaagttaac ggggatatac gcgttcagat aacagatgcc agtcggcaaa
     9721 ctctgtgtaa cgcactgaaa ctacagccag acgaagacgg gattggcgca acgctccagc
     9781 ctgcgtaaaa acacaggcgc accccgtgaa ggttgcgcct tgtctttatt agcgggatgc
     9841 tcgttgcaga atgatggctg acggcagacg ttgcaggtac cgcatacgca gcatcatcat
     9901 aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg
     9961 ttcaacgacc agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta
    10021 agccgtaaag gtgatataga aaatggaacg cgtatcttta taaccacgca aaatcccact
    10081 gccaatcacc tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc
    10141 cgccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga
    10201 aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc
    10261 agcggtttgc gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac
    10321 cgctgccgcc agcgacattg gaagcacgaa cattagtgaa ctaaagttca gggcaatctg
    10381 gtgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag
    10441 tgtcacttca aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat
    10501 aaccgcagga tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tcgagcgggc
    10561 gcgtttaatg taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac
    10621 gccacaacca acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt
    10681 caccggaatg ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc
    10741 cagaccttca cattggttac gggcaacctg gaagaaaaga tagcccggcg cgccccacag
    10801 caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga
    10861 gcggataatg taacctgcat tccacagcac cagcataatg agaacggaaa caaaacccgc
    10921 cagccagaag ccctgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa
    10981 ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag
    11041 ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat
    11101 cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc
    11161 taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg
    11221 tagttatatg agagactaaa aagccgcctg aataggcagc aaaaagaaga agcaggggaa
    11281 attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagc cattcttacc
    11341 ccttcactgg taagggcgtt ttccacctgc tattgtgctg aacagaatgt cttaactgat
    11401 ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat
    11461 tgacgagaaa ccaaattttc gcacacatgt ggtggagtta cccgaccaca tgctggacgg
    11521 tctggaaacc ggtgcctccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa
    11581 cggcaaccat gtcagttttg acctgatgaa agaaacgttg cgcattacca atcttggcga
    11641 tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg
    11701 cggacactta atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc
    11761 ggaaaataat cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct
    11821 gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac
    11881 gcgtttttgc gtccatttaa ttccggaaac tctggaacgc acgactcttg ggaagaaaaa
    11941 acttggcgca cgcgtcaaca ttgaaatcga tccacaaact caggcagtgg tagatacggt
    12001 agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa tcaggtacag aagcctgatc
    12061 tgaaatcgcc cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt
    12121 tttcaacacc gcgtgagaac accacctgcc agagctgaat atcacgggcg cggaaagcac
    12181 ctgcgcaggc attcagataa taggtaaaca tccgtttaaa gcgttcactg tagttatccg
    12241 caatttctgg ccatgcggcg acgaatcgtt cataccacgc catcaacgta gtatcgtaat
    12301 cagcaccgaa gttatgccag tcttccatca caaagtgggg ttcgctggac tgagcaatct
    12361 ggcgtacaga gggcaggcaa ccgttcggga aaatatattt attaatccag ggatcaacat
    12421 tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa tatgccttcc ggtttcaaat
    12481 tacgatccac caccgtaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc
    12541 ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg
    12601 tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga
    12661 cgcccaccac gcttacgtca taattagatg ccatgtagtg tgccagtccg ccccagccgc
    12721 agccaatatc cagtacgcgc atcccaggtt ttaactgcaa tttttcacaa atcattttga
    12781 gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt
    12841 gcatgaaggg atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctctttgc
    12901 cgactatcca ggcacgtttt ttactctgca gattgaagag acgggcgccg gcaatacgca
    12961 gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa
    13021 aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca
    13081 aagagccttc ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg
    13141 gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc
    13201 agttgtcatc cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac
    13261 tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc
    13321 gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacccttgc
    13381 cgaaatgacc atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta
    13441 tcgtagcttt aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt
    13501 ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac
    13561 ttcggcattg ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag
    13621 cgcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat
    13681 actgatcagc caggaaacta ccagacttgc gaggaagcac agacccagtt gcagagtgtt
    13741 ctgcaacgct gcggcgcgac cagttgcgtg tgggaaggga cgcagagcct gggcgacaac
    13801 aatagggtag atcgcgccat tagcaatcgc catcacacag aatgggatca ggatttcgac
    13861 cagcgacaca tggctaatga agcccgcagc ccaggtcgca atgacgctga cagcaaacag
    13921 caccagcaac cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc
    13981 ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg
    14041 gctgtagccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc
    14101 tgaacaggcc gcgtatatca gcacattgcc gcgataggtt ttagaacgta gcaggtcggt
    14161 aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat
    14221 aggcagaatc agcaccacgg taatggcaaa aagggtggcg aaaatcgcct gccaggaaaa
    14281 atggaccagc agccagcttc ctaacagagg agccagggcc ggagatagac ccaccagcgg
    14341 catgatggtc gcaaaaattc ggttaacttt ctgtgaagga taataatccg tcactaacgc
    14401 ttgccagata accgccgcgg cgcagacacc cacagcctgt acaaaacgca ataccagcag
    14461 catagcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc
    14521 gattaataat accggtttac gaccataacg atcggagagc ggcccccaca gaagctgggc
    14581 tgcggcaaaa ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg
    14641 caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag
    14701 tacgctcaaa cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta
    14761 aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt
    14821 aatatttgat gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact
    14881 cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca
    14941 tcgcgttcct tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc
    15001 gctgtttgaa cgacggcacc gtgatgtgga gctgaccgct gccggagcgt ggtttctcaa
    15061 agaagggcgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc
    15121 gaacggctgg cgcggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac
    15181 acggcagatg atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca
    15241 ggaagtgttc aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg
    15301 cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag
    15361 ctggagttgc gttgttgcca gccatcaccc gctggcgttg atggatggcc cgttcagcga
    15421 tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa
    15481 gcgtattacc tggttgctgg ataaccaaaa aagagttgtc gtgcctgact gggaatcatc
    15541 ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc
    15601 gtggcttaat gaggggaagt gggtagcatt agaactggaa aacccctttc cggattcggc
    15661 atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggt tgcttgagta
    15721 tctcggcgat agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc
    15781 aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat
    15841 caagcgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacta tacgatccaa
    15901 caacatgttg aacgctgttt cacccaacga atcttttggc tgatggattg ttgtcagcgc
    15961 cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg
    16021 gacgcgcagc cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc
    16081 acagaagacg gcagtagggc gatgcggctg cgacaggatt tgctgcatgg cgcgataacc
    16141 ggattcaggt tcaaagtcac cctgcacaat ccagctttcc ggcaccttga tcattgcttc
    16201 ttccatcgcc ttcataaaac cggcaaggcg gcctgcgcca gtgttacgtt ccagcgggcc
    16261 ggggataacg ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc
    16321 gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc
    16381 catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta
    16441 ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc
    16501 ccgctgtttc tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa
    16561 gcaatttttt tcaactgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc
    16621 tagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga
    16681 gtagtgtaat tctttgatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg
    16741 tgttttgttg atcacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc
    16801 ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa
    16861 ggtaaacgtt tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa
    16921 acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat
    16981 tttggccgat cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg
    17041 agattttttc atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa
    17101 aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct
    17161 cggtttgatc gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc
    17221 gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt
    17281 ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg
    17341 taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc
    17401 gattggcgcg tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat
    17461 tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt
    17521 tggcgtaatg gttggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag
    17581 tgcgttgatt ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc
    17641 ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt
    17701 tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt
    17761 tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg
    17821 gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat
    17881 ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt
    17941 gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt
    18001 actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac tgcaaagtat
    18061 tacccacgca acaccggtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc
    18121 tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg
    18181 ctttttgttg ctgctgatgg tgattatgct ggcaatcccg ttcctggccc gcaataagtt
    18241 cggcgcagct attagcatgg cggtgtgggg cgctgcaacc tttgcggtcg taccgccgtt
    18301 acagatgcgc gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat cagtcaatat
    18361 tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc
    18421 tgggctggga tacagctttg tgccggtgat gggggcgatt gtcgcgggac tggcattatt
    18481 gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt
    18541 aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc
    18601 ttgccgaatg cgctgttaac gcctcatccg cgttggcatg gtaagcgaag cgcatcaggc
    18661 aatactgcat ttgccatcag ttattatgca gcgagatttt tcgctacgaa ttcccagttc
    18721 accagcgccc agaagtgctc aagatagcca ggacgtgcat tgcgatagtc gatgtaatat
    18781 gcgtgttccc agacatcaac ggttagcagc ggagtcgcat cggtggtcag cggagtaccc
    18841 gcgttagagg ttgaaacgat agccagtttg ccatcgctgt ttttcaccag ccaggtccag
    18901 ccagaaccaa agtttttgat cgctgcatca gtaaactgag ctttgaaatc ggcaaagctg
    18961 ccaaaagatg cggcgatagc ttcagcgact tttccagtcg gttcgccacc ggcgttcggt
    19021 gccaggcagt tccagtagaa agtatggttc cagacctgag ctgcgttgtt gaatacgcca
    19081 ccttcagagc tgcgaataat ctcttccagt gatttacctt caaacgcggt acctttaatc
    19141 aggttgttca ggttagtgac ataagtctga tgatgcttgc cgtagtgata ctcgatagtt
    19201 tccgcagaaa tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg taattcgaat
    19261 gacattgcta ctctccttta ttattaattt gcatacgtac aatagcctta ttgtgcgtat
    19321 ggatagggta gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt gccgtaacaa
    19381 cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc cggcgagcgc caacatagtg
    19441 gcgctgccag tagtcttcac tgagagaagt gatttgaatt tcctgacctg tgcgcggtga
    19501 ctgaataaat ttgccgttgc cgacatacac gccgacatga tcggctgtac cgcgtccctg
    19561 agtacggaaa aagaccaggt cgccgttttt cagttcacta cgttcgattg gcgctgcatc
    19621 gcgcaggtga tacatttcat tcgccgtacg cggaatacga attttcacca aatctttata
    19681 agcgtaataa accaggccgc tgcaatcaaa accggtacgc ggtgagctgc caccccaacg
    19741 atatggctta ccaatttgct gcatcagttt attcattgcc acttttgtgg ctttttgcac
    19801 cttcgctttg tgtgcgtcgg caagggttcc tgacgctgca tttttgactt tcacacaatg
    19861 cgatttatag cccttacgtt tggtacactt ttcggtaaag ctggctgagg ccgttttatt
    19921 gacggtatgt ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag attttttaac
    19981 ggaagaagcg gttttgcttt tggtcgtact tttactggcg gcttttttcg ctgttttctg
    20041 ggtctttttg gtggttgccg tgcttttctt tttatctgct tttttggtga tatgagtggt
    20101 agcagaactc tccctggctt gctttgaagc atgggccata ggcgttaaag gcagggtggt
    20161 aaaaagtaaa gcacagagcg tgatcgaaat acggtttatc cgcgccactg agcgtccctc
    20221 tgttaaggca ggtctatcat gcctgcgtct gatttacaaa acttgatgat tctaatgcat
    20281 agaagaagag aaagttaata gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa
    20341 aaaaatgcag cagtgaaagc taaaaatggt cataaatacg ccagtttgcc gtgcatcagg
    20401 gaagaaacga atatttgaaa tgcaacaaat agagcaaagg gataaaatag caaaagcgtg
    20461 atgaaaatgt tattttccga tgccgcactg acccgctaca atgtcagatt actgccgtaa
    20521 agaagtttga ggaagcaaga caatgagcac cactatcgaa aaaatccaac gccagattgc
    20581 tgaaaacccg atcctgctgt acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc
    20641 tgcccaggca gtccaggcgc ttgccgcatg tggcgaacgt tttgcctatg ttgatattct
    20701 gcagaatccg gacattcgtg cggagctgcc gaaatatgct aactggccga ccttcccgca
    20761 actgtgggtt gacggcgagc tggtaggcgg ttgtgatatc gtgatcgaaa tgtatcagcg
    20821 tggcgaactg cagcagctga tcaaagaaac tgccgctaaa tacaagtctg aagagccgga
    20881 cgcggaataa tccgtcctgc aatactgacg gatgcgaacg ggcatccgtc agtcctttct
    20941 ctatccccaa tccagccctt gtggcgaact tgaaaatccc gcttcacgca gcagcgtaaa
    21001 catcggcgtt tgccagaccg gtagatcatt cacttctgtt agcgtaaagc gcagccgtgg
    21061 ttcgcgacgc agtgcggtag tcagcgcgtg gaaaacctct ggggcgagta attcctcttt
    21121 ttcctgccag accagcattt ttttgccacc ttgcgccaga tagagtaaca atttgccgcc
    21181 agaaacgacc accagcgcac ccgcccgacg cgttggtacc agcgttgccg gatgtgcagg
    21241 ccagggcaga agatttcccc acacattagc cggatcgttg gcggagagcg ccactggtgt
    21301 atagtggcgc gtttgcgcgg cttgtgtcgc caggtcgcgc aagcggtcaa tagtcagacg
    21361 ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga cctcgcataa ttcgcccgga
    21421 atcttccata cttcgacaaa gcgtttgcat cgatggaaac ccgccaggga tattttcggc
    21481 tatcaccgcc tgacgactga tgatgccgta gcggtcgagc atattttccg ccagcgccag
    21541 catcctttcg gtatcgttta gtggctccac ctgcaataac gaccagcgtc cagccagatt
    21601 tggtgtgttg taagataccc gcggcgagac gggttgtgca tagacaggac gcccacgacg
    21661 agcccggtga ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg
    21721 taacggtgcc caaatatcgc tggtgatgac gccttgccag accagcgccc ataacgcctc
    21781 ttgcagtgcc gagggatcaa ccgattcgcc gattttgtcg cgtatccgtt ggctgatttg
    21841 ttgtgcaaac caggctcctc cgtcagccag aacagcgact atcgcttgtt gcagcgccga
    21901 acgattcgcc tgatccgctt cggcgggagt gaacgattct gcagcatatt cctgtagatg
    21961 cagtgccacc aggccgtcat cttcacccag ttttttttgc cccgaccaga taaccgcacc
    22021 ggttgccagt aattcatcga gcacttctgg cgaatagtcg tgtacgcggg caggcaagat
    22081 ctggctttcc cagagtgagg cgggtaagcc gactccggca agctgttcga tcacccgcat
    22141 cacgccatcc accccctcat aaacgcctgg cgatgttgag gcaaagagcg ccgggctacc
    22201 atcggtggcg ggtaatacgc cctgacgttc cagcagcaat cgcgcatagg tcgtggctgc
    22261 aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac gacgaaatac
    22321 ttcatcgctc acccagatgt cttgttgcag attcatcacc agaccctgtt cgcgcagttg
    22381 ctgaagctgc tcttcgacaa tggcaatacc gagactaaac tcatgagcca gttgttcagc
    22441 cgtgaccaga gcatgagccc ggagatagcg tagaaagagg tcgcgaagcg ggtaactgac
    22501 tctatgcaaa taaatctctg gcagtgactc tggtaatcgt acgccgaggg catcgcgcag
    22561 cctggcggca tcatccatac aggcaagacg ctcctgtcca ctaatcatcg tcgggaagat
    22621 gcgttttact gtaagaagat tttccagata cgaggcaatc tcttcactgc tgcctgtatg
    22681 ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc
    22741 ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc aactcttctt ccacctggcg
    22801 aatgacctgc gggtcgagta attcccccgg atcgacctgt ccgagtagat tgcgcagtaa
    22861 ctcgctgtcc agcgacagta cggatgcccg gcgttctgcc agcggggcgt cgctctggta
    22921 cataaattcc gcgacatagc cgaacaataa acttgtggcg aaaggcgagg gcgtagtagt
    22981 cgttacatcg gatatttgaa tttcgcctcc gttcaggcga cgcatcaaac gttccagtgc
    23041 gggaagatca taaacatctt gcagacattc gcgtagggtt tcgagaatgg ccggaaaatc
    23101 cggatatccc tgagcgattt ccagcaactg actggcgcgc agccgttgtt gccaaagcgg
    23161 ggtgcgatgg cccggcgtgc ggcccggcat taataatgcc cgcgcggcgc attcgcgaaa
    23221 acgggcggcg aaaagtgccg aactgcctac cgcctcgcgg acaatttgca gcaacttttc
    23281 tggttcaaac aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc
    23341 aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg ccccaaagcg catgtattcg
    23401 cccggcaatc gccagcgccc agggttcatg cacccgtctt ccatagggag agtgcaaaat
    23461 aatacgccaa tcgccaattt catcacggca ccgttcgagg accagatgac gactccccgg
    23521 aacaataccc gtcgcgttgc gctgctcgtc aatcaacccc tgaatattgg cgatcgtatt
    23581 ttcttcagcc agccacgggg gaatagtccc ggaaaagaac gctccatccg ccagcaaatg
    23641 aagaaaatcg ccgatcatct cgcctaattc agccggacgt ccgttacctt caccacgcca
    23701 gaaggggagc cgggcagaac gacccggagc aggagtcaca atcacctgat cgcgggtgat
    23761 ttgctggatc cgccatgagg ttgcgccgag agtgataata tcgttcaccc gcgactcata
    23821 taccatctcc tcatccagtt cacccacccg tcgcgaaccg gcctgttctt ccccttcggg
    23881 taataacacg ctatacatgc cacgatccgg aatggtgccg ccgctggtaa ccgccaacag
    23941 ctgagcgcca ggtcgggcgg tcaatatccc ggtctccctg ttccagacca gtttggggcg
    24001 aaaagcagaa aaatcgccag agggatagcg tccggaaagc atatccagcg tggcgtcaaa
    24061 aacacgtctt ggcagatctt tccacggtgc ggcacggcgt acgcgggagt accattcgtc
    24121 tacctgtaat gcatccatcg ccgcggcggc aacggtgtgc tgcgcaagga cgtcgagtgg
    24181 attatgcgga ggtgtcaggt tttccagcct gcctgcgaac atacactcta caatgactgc
    24241 ggaatcgact aagtcacgcc tggttcgggg gaaaaaaagc cctttggaga tacctcctat
    24301 ctggtgtccg gcacgtccaa tgcgttgtaa cccactggca acagaaagcg gcgttgccac
    24361 ctgaatcacc agctccacag cgcccatatc aatccccagt tcgagactgg aagttgcgac
    24421 cacgcagcgt aactcacccg atttcagcgc ctgctcggtg attgctcgtt gttctttaga
    24481 gacggagccg tggtgtgagc gggcaataaa aacgtcgcta ctttgtacac ggttagaggt
    24541 tgcgccagag gtcgactcga aatgggccgc atcagcggcg atagacgggg aacgctgtaa
    24601 gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc cccgcgaatt
    24661 agtaaagaca atggtcgagc gatggcgcaa cacttcatca aggatacccg tttcaatata
    24721 tggccagatg gagccttccc ggccggcatg gctgtcttcg ccagtgccgc tggcgaccga
    24781 tgagacatca tccatattgg cgactggtac gacaatccgt atctgcggat gacgcattgc
    24841 gggcgggttg actaccgtaa ccgggcgatc gccaccaaga aatgctgcca catcgctggc
    24901 tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag
    24961 ccgctccaga cttaacgcca gatgcgcacc acgtttactt cccgctaccg cgtggacttc
    25021 atcaataatt accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag
    25081 atagagtgat tcgggtgtgg tgatcagaat atccggcgga ttacgggtga gtttgctgcg
    25141 ttcctgtgca ggcgtatcgc cagtacggat ccccacgcga agattgactt ccgtttcgcc
    25201 gcgccgccgc cgttcatcgg caataccctt caacgggatc tgcaagttgc gctgaacgtc
    25261 ggtgcccagg gcttttatcg gtgaaatata gaggatgcgt gaggttttac gtttatgcgc
    25321 ctcgcgggta tcttcgccgc cttcgcggaa gagtcgatcg agggcgtaga gaaatgctgc
    25381 cagcgttttc ccggagccag tcggtgcaat caccagcgca tgttcgcctc gcgccgccac
    25441 atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg
    25501 ggtcgccggt gaaaacacgt ccggcagggg cgatgaaggg tttggattat ctgccattaa
    25561 cgctattccc cgtgaatatc atgcatgatg cgtggaatag cctcactata gatgcctgaa
    25621 tcattgcatg tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag
    25681 cctcccagac gtttccagcg gttgacgatt tcacaaaaca gcaccgcagt gcgttcggtg
    25741 tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc
    25801 tttgacaata cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg
    25861 aaagggtgga acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg
    25921 tgatcgaaat tggcgttgtg cgccaccata atggcgcggt tacagccgct cgctttaata
    25981 cctttacgta caactttaaa aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga
    26041 tcgggatcgt tcgggtcaat gccgttgaaa gcaagggctt ctggttgcaa atttgcgccg
    26101 acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc
    26161 attttcaggg tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccagct
    26221 gtttcaacat cgatcacaac aggataaaag ccacgaaaac ggtcgcacag accggtaagt
    26281 tgagcgttat cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca
    26341 aatattactt gtcgatgcag taaagatgcg ggcgcgatga gttcgcgccc ggcaggagat
    26401 tagttgccca gaccgcgacc ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc
    26461 ggatcttcca caaacgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc
    26521 acgttacccc cgttttgacg gattttttcg cacgcttcag cggcattatc tacgctaagc
    26581 gcgatgtgac cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt
    26641 tcaatcaccg cttcttcggt ttccgggccg tagccaacaa acgccagtga gtatttgtat
    26701 tccgggtttt cgctggtacg cagcagtttc atgcccagca ctttggtata aaaatcgatg
    26761 gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tttatcctca
    26821 aaatggctaa cagattcaaa atgttgtttt agtatagcgg cggctttacg ccgctcgcaa
    26881 tgttggatta caacgtcggg taatcagtgt agccttccgc accgccacca tagaaacttt
    26941 cagcacgctg tgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg
    27001 caatccagtc acgaccaaat gccaccgcat caattaaccc tttgccgatc agcgtttcag
    27061 ctttttctac tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt
    27121 tttcgcggaa cgcatcagta tacggttcac cccccgccca atctggttct gacatatgca
    27181 gataagcaat gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat
    27241 tcgggccgtt atccgtgttc tggaaagtac cgattggcga aacgcgaatg ccaatgcgat
    27301 cggcacccca ttcttcaatc ccggcatcga ccacttccag taccaaacgt gcgcgatttt
    27361 ccacgctgcc gccgtactga tcggtacgat ggtttgaaga aggagaaagg aactgatgca
    27421 gcaaataacc atgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag
    27481 caatagcctg acggaaatca ttgacgatac ttggaatctc ttccagttca agcgcacgcg
    27541 gcatggatgt ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc
    27601 ccgcgctaag tgctgaaggc gctactggtg cctggccgcc aggttgcagg ctggcgtgag
    27661 aaatgcgtcc ggtgtgccac agctgtacgg ccatatgccc attttcagca tgaacgccag
    27721 cggtaatttt tttccatgcg gcaatttgtt ccggactatg gatgccaggt gcaccagcat
    27781 acccttttgc ctgggcagaa atttgcgtgg cttcgctaat aatcaaaccg gcactggcac
    27841 gttggcgata gtattccgcc atcaacgggg tagggatgtc acccggttca atactgcgca
    27901 gacgcgtcag cggtgccata aaaatacggt ttgccgcagt tattgcgccc actttcagtg
    27961 gggaatacag tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct
    28021 gctaaacggc aggcgtcgca ataatggttt ttacatgtgc cagcgcgttt tccagtggct
    28081 cgtaattgcg cgaaattttg gcctgcagat tcgcacccag ccatagtgcg taaagcactt
    28141 gtgcctgttg cagcggttcg ccacaaaagg ttaaacaatg gctatcacgg ccattttcca
    28201 gagcctgcga gagcagggcg atcacgccgc gagcgccttt atccatcgcg ctacgcatat
    28261 cttctgacag atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc
    28321 catgttggca aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt
    28381 taccttcgcc ggattgcagc aactcagtca gtcgttggtg atatgtggcg taatggcgct
    28441 caagcatggc aacgccaaac gcttctttag agcgaaagta gtgatagaag gaccctttcg
    28501 gcacttcagc ggtttttagt aattcgctta gccccatccc ggtgaatcca cgttgcaggc
    28561 aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca
    28621 tgcgttggag tgtagtagac cagtcggtct agtgcaagca aagttgctac tttgggcatt
    28681 aacagcttca ataatggtaa taatcttatt tgaggagggc gaagtggcgg agcaattaga
    28741 gttctttccc gtccagagcc cgtgccgggg aatttgccag tctgacgaac gcgggttttg
    28801 tcgcggctgt tttcgaagcc gggatgagcg ttttaactgg aataaaatga gcgatggcga
    28861 aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg cgcaaattac gtgcaaataa
    28921 gccagcgtca tcagacgaac cggagcaacc atcactcttt tgagcctaat cttgcgtata
    28981 ctcctctcat atctgataag aggaagcgat tatggttcag cgtattacta ttgcgccgca
    29041 aggcccggag ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaacatgtc
    29101 cgcccgccag ctggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga
    29161 ccatgctgat atttatggtg gctatcagtg cgaagcggcg tttggcgagg cgctgaaact
    29221 ggcacctcac ctgcgtgagc ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc
    29281 gcgtgaagaa aacgtcattg gtcattacat cactgaccgc gatcacatca ttaagagcgc
    29341 cgaacagtcg ctaattaatc ttgcgaccga tcatctggat ctgctgttaa tccaccgccc
    29401 ggacccgtta atggatgccg atgaagtggc ggacgcgttc aaacatctgc atcagagcgg
    29461 caaagtgcgt cattttggcg tatccaactt cacgcctgcg caatttgccc tgttgcaatc
    29521 acgtctgccg tttacccttg ccactaatca ggtggaaata tccccggtac atcagccgtt
    29581 actgctggat ggcacgctcg accaactgca acaactgcgt gttcgtccga tggcgtggtc
    29641 ctgccttggt ggtggtcgtc tgtttaatga tgattatttc cagccgctgc gtgatgaact
    29701 ggctgtggtg gcggaggagt taaacgcggg ctcgattgaa caggtggttt acgcctgggt
    29761 attacgttta ccgtcgcagc cgctgccaat tatcggctga ggtaaaattg agcgcgtacg
    29821 ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa caatggtttc gtatccgtaa
    29881 agcggcactg gggtacgacg taccgtaagc acttttagga atagccgccg ttcaaaaatg
    29941 tgtcactggt ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt
    30001 agtctggcca ttctggcgct ggttgttgcg accggcgcac aagctgccag tgaaaaagtc
    30061 gagatgaacc tcgtcacgtc gcaaggggtt gggcagtcaa ttggtagcgt caccattact
    30121 gaaaccgata aaggtctgga gttttcgccc gatctgaaag cattacctcc cggtgaacat
    30181 ggcttccata ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc
    30241 gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca
    30301 gaaggtgccg ggcatttagg cgatctgcct gcactggtcg tcaataatga cggcaaagct
    30361 accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg aaatcaaaga caaagcgctg
    30421 atggtccacg ttggcggcga taatatgtcc gatcaaccta aaccgctggg cggtggcggt
    30481 gaacgctatg cctgtggtgt aattaagtaa ttaagaggcc agcgtacctt gcggtggtgc
    30541 ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca attgccgcca gctcacgggc
    30601 ggcaggttga tgatggcggg caaggctgtc gcaaatccgc tgtaattctt caagtgtggc
    30661 cgccagtgat ttttgctgaa cgccacgctc actcatcact ccccgcaaca gtgaaataca
    30721 gttatcccgt actcgcgata acggatcgga acgcgattcc cagtcgcgta attgccagac
    30781 aacatgagaa cagttcagca gcactatacc ccagcgtaat aaccaacggc gagccagcgc
    30841 atcctggctg ttactcaact gactgacgtg atgataagtg agcgattcaa attcgctttc
    30901 actcagtgtt ggatggcggc ttagttgatc gacaaaatcc cggcgcagcg cgcgaatatg
    30961 gcggcggctt ttgcgagcat ccgatcctgg acgcagaatg gcgaacgcta accacgccaa
    31021 cgcgacgcca acgatttttg ccagattatc gttaagaaaa tcagcaaaat tatacaccgg
    31081 gggattagtg acggcgataa aagaacccat aaaaacaatc agttgccccc acaatgcggc
    31141 aaatttgggc atctgcaatt taagaagctg cattgtcgcc agcagtggaa agagaaacag
    31201 taaaaattgc cacagatcgc taatctggac catcagacca aatttgacca caaagctgaa
    31261 tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac gacttaaacg gtgctgcgac
    31321 ggcggagtag agtacgcagc taatcgctgc cagcgttaat gcattggcac cggcatccca
    31381 ttgcgaagca atactccatg cgccaatcat catcaacgta caaaatgtac gcagtccgct
    31441 ccacatagct tcggcgttat cggtatgacg agccaggccg gatgtccgtg ggagtctggc
    31501 atgatcatct acatcgcttt gcagacgatg taattcctga ctactttgca gataaaggcg
    31561 gcaaaaataa cgtagtcgct gccagaaggc gacgtgccga tagtcggcga cgttggtcgg
    31621 gcgtagcggg gcgataatac gtgcgacggt gtaaacatct gtttgcgaac tggcgagcgc
    31681 cgtcagcaac tgttcgagaa tttctcgcgt ggcacctggc ggtgagggcc agttgagcaa
    31741 catacgtcgc aggctggaga tgacactggt catacgtaat tgctggtgga gcagcgcatt
    31801 aaggcgcgcg ttttgctggc gaaaacgata gtggctccag aaagcctgga tacgcagcaa
    31861 attcatggtc agtatctgcc caatcacccc ttcatgtgct gcacgaatgg catcggttgt
    31921 ttcaggctgc cagagtaaac tggtatgttc cagtaatcgg gcgtgcatgt ttttcaatgc
    31981 ggttaaaagg gctgtagcat cggaactgct aggcaggatc atcatcatca tgccgccgca
    32041 caaaataccg acaatcacct cgcaaacgcg cgcctgagcg atatcccaca gctggctggc
    32101 ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa
    32161 tgcatacgcg acgttattcg tgaagtgcgc acaggcccag gtacaaaagc caagccacgc
    32221 cgacatgctc aatagaaaaa accacggctc attgagcgta tgcccggcaa gaagcagtgc
    32281 cgcaatggct cccagtaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc
    32341 aacggtggga aagctaacca ctgcagccga ggtcatcgcc caatagggtt catccagatt
    32401 caaataatag gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg
    32461 ccattgcgcc agcgtggccc tgaaccaggg caaattgcgc aaggaccatg acgatgcgtt
    32521 cattattgac ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac atcgcctgga
    32581 actttatcca gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca ggggacgtta
    32641 ggtttcacat ccggaataag gcttgagtca ctttcaacgc tctgatcata aatcgcccga
    32701 ccgatactgg aaacgtgacc ctgtaacgtt ttgttatcac tatagagggt aatttgtgct
    32761 ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac
    32821 gagtggctgt cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc gccgatgcgg
    32881 gtagtgagat tagtcaccca tccactcacc ggggcgcgaa tttccgtttg cgccagttgc
    32941 cattgtgtct gtttcagcgt cgcctgtgcg gcatcaacac tggcctgcat cgctttaaca
    33001 ttgaggttag cggtatcgag ttcttcggca gaaataaaat tttgtgagag atgacgacga
    33061 cgattcgctt cgttattggc ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt
    33121 gcattcagct cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg
    33181 ttaaccagct ggttgtcttt tatattcagc tcaacaatac gtcccgacac ctgtggggtg
    33241 atagaaacct gttctgcgcg tattttcccg tcgcgtgtcc acggagattg catgtaataa
    33301 ttccacagcc accatccggc aagaaccgca actaccgcta caatgattgt tgagaaatac
    33361 ttaattgttt taattgacat agttaccacg caatcagtat tgccagagca aggcaaacgc
    33421 aaatcgcaaa cagcgataaa tccattaaca agggatgcca gatgtcaccg gcgtagatcc
    33481 agccacgaag caggcggtgt acgacaagcc agatgacgaa tccaaacgcg aaggctttga
    33541 aaaacggagg aaagtagacg gacgcaccga acaccagatc ttgtaagggc aatcctgttg
    33601 cattgagcat aaacttcaca aagtagattc ctttacgacc gaatatattg cgttagatta
    33661 ataaatattc tttaactgcg aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt
    33721 aaccaaatgc agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc
    33781 cgcataatat cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct
    33841 gatctggcac ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg
    33901 gagttaacac aaacccattg ggttacgtta cacaatatcc atcagttacc tccagaccag
    33961 tcgcaaattc aactggcaaa agcgattggc atcgagcagc catcactggt ccgtactctg
    34021 gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct
    34081 aaacgtatta aactgacgga aaaggcagag ccgctgatca gcgaaatgga agctgttatt
    34141 aacaaaaccc gcgcggaaat tttacatggc atctccgcag aggaactgga acaactgatt
    34201 acgctcatcg caaaacttga gcataatatc attgagttac aggccaaagg gtgaaatgaa
    34261 gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga gaaacggtca
    34321 cctgactgcc attgctggcc agtacgacac gttggcccgg agagaaacga gtgttgcctt
    34381 gtttctgtac caccatgatg gtattaccat cgtctttacg aatttccagc tcgacacctt
    34441 gcgttttgtt cattgcactc tgtacgccct gaccagctac gccacctgca acagcgcctg
    34501 ctgcagtagc cagagaacgc ccggttccgc caccaacagt atttcccagg aaaccaccaa
    34561 gaacagcacc gccaattgca ccgataacgt tggaatcatc accgccctga atctgtaccg
    34621 gacgtacgtt aacgatggtt ccatagctga cattctgtac ttgtttcgct tcagaagcgg
    34681 tataaacatc ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca
    34741 ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac
    34801 gcaagcatcc ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac
    34861 caaatatgag caaatcataa tgaaagtagg atcaaccaat tataaacaaa acattcatga
    34921 tgattattga tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg
    34981 gtattcacga aaagtttatg tttgagtgtg cgcaaacgtc agattgaaaa aggatgaact
    35041 atgaaatcgg gccgctttat tggcgttatg tcaggcacca gccttgatgg tgttgatgtt
    35101 gtgttggcga caattgatga acaccgggtc gcacagctgg caagtttgag ctggccgatc
    35161 ccggtgtctc tgaaacaggc tgtactggat atttgccagg gccagcagct tacactttcg
    35221 cagtttggac agcttgatac tcaactcggg cgactttttg ctgatgcggt caaggccttg
    35281 cttaaggaac aaaacctgca ggcgagagat atagttgcga tcggctgtca cggtcaaacc
    35341 gtctggcatg aaccgacggg cgtggcacca cacactttac agattggcga taacaatcaa
    35401 attgtggcac gcaccggaat tacggttgtc ggtgattttc gccgtcgcga tattgccttg
    35461 ggaggacaag gcgcaccgct ggtacctgcg ttccatcatg ccttgctggc tcacccaacc
    35521 gagcgacgaa tggtgctcaa tattggcggc atcgccaatc tgtcactgct cattcctggg
    35581 cagccggttg gcggctacga taccggtcct ggtaacatgc tgatggatgc ctggatctgg
    35641 cgtcaggccg gtaaacctta cgataaagat gccgagtggg cacgggcggg taaagttatt
    35701 ctcccactgc tgcaaaatat gctcagcgac ccatatttct cgcaacctgc accgaaaagc
    35761 accggacgcg aatactttaa ctacggctgg ctggagcgcc atttacgcca ttttccgggt
    35821 gttgatcccc gtgatgttca ggccacactg gcagaactca ccgccgtgac catttctgaa
    35881 caagttttgt tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg tagtcgtaat
    35941 ccgctactca tggcgcgtct ggcggcatta ctgccaggca cagaagtcac caccaccgat
    36001 gccgttggca ttagtggcga tgacatggaa gcattggctt tcgcttggct tgcctggcgg
    36061 acgctggcgg gattaccagg aaatctgcct tccgtcactg gcgcaagcca ggagacggtt
    36121 ctgggggcta ttttccccgc caactcgtga tatcagaatt aactgaattt tttcttatcg
    36181 tgtctgccgt tacacttgga agattaagga ggatatttgc cctccggacc aggaaagtct
    36241 tcgggatatg actatgaaaa aactgttaat tatcattttg ccggtccttc tctccggctg
    36301 tagcgccttt aatcaactgg ttgagcgaat gcaaaccgat acgctggaat accagtgtga
    36361 tgaaaaaccg ttgacggtca aactgaataa tccgcgccag gaagtcagtt ttgtttacga
    36421 taatcaacta ctgcatctca aacagggcct ttcagcctct ggcgcgcgtt actccgacgg
    36481 gatctatgtt ttctggtcga aaggtgagga agcgactgtc tataaacgcg accgcatcgt
    36541 cttgaataac tgtcagttac aaaatccaca gcgttgagat ttttccaggg gcggcgcaca
    36601 atagcgtcac ccacagacaa tccgtaaaga aaaccatgtc tgataacgac gaattgcagc
    36661 aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt acgccgccgc gatcttcccg
    36721 ccgatccatt aacccttttt gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg
    36781 accctaccgc gatggtggtc gctaccgtgg atgaacatgg tcagccttat cagcgcatcg
    36841 ttttactcaa acattacgac gaaaaaggca tggtgtttta caccaacctc ggtagccgta
    36901 aagcacatca aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg catacccttg
    36961 aacgccaggt gatggtgatc ggtaaagcag aacgactttc gactctcgaa gtgatgaaat
    37021 attttcatag ccgcccgcgt gatagccaga ttggttcatg ggtttcgaag cagtccagtc
    37081 gcatttctgc ccgcggtatc cttgaaagta aattcctgga gctgaagcag aagtttcaac
    37141 aaggcgaagt gccattgccg agcttttggg gcggttttcg cgtcagcctt gaacagattg
    37201 agttctggca gggcggtgag catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg
    37261 atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc
    37321 tggtactcct gattctggca ctttattcta tgtctctttc gcatctggcg aaaagtcgtg
    37381 taccggcaaa ggtgcagtcg ttatatacat ggagattttg atggcaagca gtaacttgat
    37441 taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag cgttagcaga
    37501 gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt
    37561 gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa
    37621 gccggttgcg ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc
    37681 cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa tccgcaagca
    37741 ggttgccccg ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg cgaacaacta
    37801 tgactggttc ggcaatatga atgtgctgac cttcctgcgc gatattggca aacacttctc
    37861 cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg
    37921 gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa
    37981 caaacagtac ggtgtggtgc tgcaaattgg tggttctgat cagtggggta acatcacttc
    38041 tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct
    38101 gatcaccaaa gcagatggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga
    38161 tccgaagaaa accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga
    38221 cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt gaagagatca acgccctgga
    38281 agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt
    38341 gactcgtctg gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct
    38401 gttcagcggt tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg
    38461 cgtaccgatg gttgagatgg aaaagggcgc agacctgatg caggcgctgg tcgattctga
    38521 actgcaacct tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa
    38581 cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg
    38641 ttttacctta ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga aataatgcat
    38701 taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg aacatgatga
    38761 aaaatattct cgctatccag tctcacgttg tttatggtca tgcgggtaac agtgcggcag
    38821 agtttccgat gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt caattttcta
    38881 atcacaccca atacggcaaa tggactggct gcgtgatgcc gcccagccat ttaaccgaaa
    38941 ttgtgcaagg cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct
    39001 atctgggatc ggcggagcag ggtgaacata tcctcggtat cgtccgtcag gtgaaagcag
    39061 cgaatccgca ggcgaaatat ttttgcgatc cggtaatggg tcatccagaa aaaggctgta
    39121 tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc agcgatatca
    39181 ttgcgccgaa tcttgttgag ctggaaatac tctgtgagca tccggtaaat aacgtcgaag
    39241 aagcggttct ggcagcgcgc gaactcattg cgcaagggcc acaaattgta ttggttaaac
    39301 acctggcgcg agctggctac agccgtgacc gttttgaaat gttgctggtc accgccgatg
    39361 aagcctggca tatcagccgt ccactggtgg attttggtat gcgccagccg gtaggtgttg
    39421 gtgatgtgac gagcggttta ctgctggtga aactgcttca gggggcaacg ctgcaggagg
    39481 ccctggaaca tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa gcaatgcagg
    39541 aatatgagtt gcaagtggtt gctgctcagg atcgtattgc caaaccagaa cattacttca
    39601 gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gaactgtggg ccgacggcta
    39661 ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg
    39721 cataaatgcc gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg
    39781 cagtaccgta aacagatagg catcagcaat tgtaaatctt tgcccgcaga tccagtgctc
    39841 atccttcagt gcctcgttca catattgcag tttcttctcc agctgcgcgc gaactgtcgg
    39901 tttgtactct tccggtgtat cagggcgaaa cagaggtgtg aaacctttat gcagctcggt
    39961 ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag
    40021 caactggcgg tcggggacgc tgtcggcaag atactgcata atcgctacac cttccgtcag
    40081 caaagtaccg tcatccagca gcaatgcagg tacctgtccc ttagggttaa cggcaaagta
    40141 atcgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt
    40201 tccgctctca cgcagggtga tatgggaagc gagagagcag gcgcccggtt tgtagaacaa
    40261 tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa
    40321 aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat tttacgctac
    40381 ggctgctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca gtttcggcgc
    40441 ggtcagcagc atcagtactg caataacggc agtagcgaca ccaatctgca agaatacgcg
    40501 accatagact tccagtgaca tcagcggatc ggtaacgtta tccggcacag ccatcatacc
    40561 cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc tacccataat
    40621 gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca gaccagagat
    40681 catcagttcc ccgatactct gcaggccata gctcgcgacc agccagctta cagacacgat
    40741 acctgcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat
    40801 caccataccg attgcaaact tggttggcat cggcagggta tcgcccatct tgttatagat
    40861 agcggccaga atcgggctac caatgatgat ccagaacggg ttcagtgcct gatactgctc
    40921 aggttctacg gccaggccca gaatggtatg ttcaacgtta cgaatcgcaa agaagttcag
    40981 tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca tcaggatgaa
    41041 ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt taccgaagat
    41101 aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag
    41161 cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca gcaggttacg
    41221 gtagttgata ggctcgaagt ctggttttga accgttctgt ttaacccagc gttggcagaa
    41281 ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca
    41341 gccgtatttc gcggccagcc acggggtggc gatcatagag aagaaagagc cgatgttgac
    41401 ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct catagcatgt
    41461 agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat
    41521 acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc
    41581 cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca
    41641 gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga
    41701 atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc
    41761 gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg
    41821 tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac
    41881 ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata
    41941 cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca
    42001 gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg
    42061 gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactgcatt
    42121 gagaggggga tgatggcttg acggcgatat gttctgctat ttagcgtttt tcctagtgag
    42181 atagtccagt ttcttaaaaa tagccagtgt aatgttttgt aggtcaatat ttgttgcgtg
    42241 tgtaaattta aatg
//
LOCUS       DBFIFS010000016        41968 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.016, whole genome shotgun sequence.
ACCESSION   DBFIFS010000016 DBFIFS010000000
VERSION     DBFIFS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.016"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            289..375
                     /gene="leuL"
                     /locus_tag="N7A07_000819"
     CDS             289..375
                     /gene="leuL"
                     /locus_tag="N7A07_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU2475541.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            468..2039
                     /gene="leuA"
                     /locus_tag="N7A07_000820"
     CDS             468..2039
                     /gene="leuA"
                     /locus_tag="N7A07_000820"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU2475542.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            2039..3130
                     /gene="leuB"
                     /locus_tag="N7A07_000821"
     CDS             2039..3130
                     /gene="leuB"
                     /locus_tag="N7A07_000821"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU2475543.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3133..4533
                     /gene="leuC"
                     /locus_tag="N7A07_000822"
     CDS             3133..4533
                     /gene="leuC"
                     /locus_tag="N7A07_000822"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU2475544.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4544..5149
                     /gene="leuD"
                     /locus_tag="N7A07_000823"
     CDS             4544..5149
                     /gene="leuD"
                     /locus_tag="N7A07_000823"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU2475545.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5257..5388)
                     /gene="sgrT"
                     /locus_tag="N7A07_000824"
     CDS             complement(5257..5388)
                     /gene="sgrT"
                     /locus_tag="N7A07_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU2475546.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5477..7135
                     /gene="sgrR"
                     /locus_tag="N7A07_000825"
     CDS             5477..7135
                     /gene="sgrR"
                     /locus_tag="N7A07_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU2475547.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7364..7933)
                     /gene="espY2"
                     /locus_tag="N7A07_000826"
     CDS             complement(7364..7933)
                     /gene="espY2"
                     /locus_tag="N7A07_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HGU2475548.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8175..8274
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8305..9288
                     /gene="thiB"
                     /locus_tag="N7A07_000827"
     CDS             8305..9288
                     /gene="thiB"
                     /locus_tag="N7A07_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU2475549.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9264..10874
                     /gene="thiP"
                     /locus_tag="N7A07_000828"
     CDS             9264..10874
                     /gene="thiP"
                     /locus_tag="N7A07_000828"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU2475550.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            10858..11556
                     /gene="thiQ"
                     /locus_tag="N7A07_000829"
     CDS             10858..11556
                     /gene="thiQ"
                     /locus_tag="N7A07_000829"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU2475551.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11643..12407)
                     /gene="yabI"
                     /locus_tag="N7A07_000830"
     CDS             complement(11643..12407)
                     /gene="yabI"
                     /locus_tag="N7A07_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2475552.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12493..13371)
                     /gene="araC"
                     /locus_tag="N7A07_000831"
     CDS             complement(12493..13371)
                     /gene="araC"
                     /locus_tag="N7A07_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU2475553.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13710..15410
                     /gene="araB"
                     /locus_tag="N7A07_000832"
     CDS             13710..15410
                     /gene="araB"
                     /locus_tag="N7A07_000832"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU2475554.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15421..16923
                     /gene="araA"
                     /locus_tag="N7A07_000833"
     CDS             15421..16923
                     /gene="araA"
                     /locus_tag="N7A07_000833"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU2475555.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17123..17818
                     /gene="araD"
                     /locus_tag="N7A07_000834"
     CDS             17123..17818
                     /gene="araD"
                     /locus_tag="N7A07_000834"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU2475556.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17893..20244
                     /gene="polB"
                     /locus_tag="N7A07_000835"
     CDS             17893..20244
                     /gene="polB"
                     /locus_tag="N7A07_000835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU2475557.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20408..23314
                     /gene="rapA"
                     /locus_tag="N7A07_000836"
     CDS             20408..23314
                     /gene="rapA"
                     /locus_tag="N7A07_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU2475558.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23326..23985
                     /gene="rluA"
                     /locus_tag="N7A07_000837"
     CDS             23326..23985
                     /gene="rluA"
                     /locus_tag="N7A07_000837"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU2475559.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24198..24376
                     /locus_tag="N7A07_000838"
                     /pseudo
     CDS             24198..24376
                     /locus_tag="N7A07_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24403..25140)
                     /gene="espY1"
                     /locus_tag="N7A07_000839"
     CDS             complement(24403..25140)
                     /gene="espY1"
                     /locus_tag="N7A07_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HGU2475560.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATVDGATHLYDEVIIINKI
                     TPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(25435..26250)
                     /gene="djlA"
                     /locus_tag="N7A07_000840"
     CDS             complement(25435..26250)
                     /gene="djlA"
                     /locus_tag="N7A07_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU2475561.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26505..28859
                     /gene="lptD"
                     /locus_tag="N7A07_000841"
     CDS             26505..28859
                     /gene="lptD"
                     /locus_tag="N7A07_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU2475562.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28912..30198
                     /gene="surA"
                     /locus_tag="N7A07_000842"
     CDS             28912..30198
                     /gene="surA"
                     /locus_tag="N7A07_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU2475563.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30198..31187
                     /gene="pdxA"
                     /locus_tag="N7A07_000843"
     CDS             30198..31187
                     /gene="pdxA"
                     /locus_tag="N7A07_000843"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU2475564.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31184..32005
                     /gene="rsmA"
                     /locus_tag="N7A07_000844"
     CDS             31184..32005
                     /gene="rsmA"
                     /locus_tag="N7A07_000844"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU2475565.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            32008..32385
                     /gene="apaG"
                     /locus_tag="N7A07_000845"
     CDS             32008..32385
                     /gene="apaG"
                     /locus_tag="N7A07_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU2475566.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32392..33240
                     /gene="apaH"
                     /locus_tag="N7A07_000846"
     CDS             32392..33240
                     /gene="apaH"
                     /locus_tag="N7A07_000846"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU2475567.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(33237..33551)
                     /locus_tag="N7A07_000847"
     CDS             complement(33237..33551)
                     /locus_tag="N7A07_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HGU2475568.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33554..33787)
                     /locus_tag="N7A07_000848"
     CDS             complement(33554..33787)
                     /locus_tag="N7A07_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU2475569.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33873..34352)
                     /gene="folA"
                     /locus_tag="N7A07_000849"
     CDS             complement(33873..34352)
                     /gene="folA"
                     /locus_tag="N7A07_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU2475570.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34544..36406)
                     /gene="kefC"
                     /locus_tag="N7A07_000850"
     CDS             complement(34544..36406)
                     /gene="kefC"
                     /locus_tag="N7A07_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU2475571.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36399..36929)
                     /gene="kefF"
                     /locus_tag="N7A07_000851"
     CDS             complement(36399..36929)
                     /gene="kefF"
                     /locus_tag="N7A07_000851"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU2475572.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37037..38368)
                     /gene="yaaU"
                     /locus_tag="N7A07_000852"
     CDS             complement(37037..38368)
                     /gene="yaaU"
                     /locus_tag="N7A07_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2475573.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38426..38713)
                     /gene="fixX"
                     /locus_tag="N7A07_000853"
     CDS             complement(38426..38713)
                     /gene="fixX"
                     /locus_tag="N7A07_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU2475574.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38710..39996)
                     /gene="fixC"
                     /locus_tag="N7A07_000854"
     CDS             complement(38710..39996)
                     /gene="fixC"
                     /locus_tag="N7A07_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2475575.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40047..40988)
                     /gene="fixB"
                     /locus_tag="N7A07_000855"
     CDS             complement(40047..40988)
                     /gene="fixB"
                     /locus_tag="N7A07_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU2475576.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41003..41773)
                     /gene="fixA"
                     /locus_tag="N7A07_000856"
     CDS             complement(41003..41773)
                     /gene="fixA"
                     /locus_tag="N7A07_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU2475577.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10056 a        11440 c        10926 g         9546 t
ORIGIN      
        1 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
       61 tcctcgtgga ttagatgcaa aaatttattc tgaaatatgt caaccgatga aaagcatcgg
      121 tagttaagca gaaattaata tcgcttgttt taaccacagc agcacaatta gctaatctta
      181 cggatgcaga actcatgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
      241 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
      301 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
      361 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
      421 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
      481 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
      541 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
      601 tcgggttccc cgtctcttcg ccgggtgatt ttgaatcagt gcaaaccatc gctcgccagg
      661 ttaaaaacag ccgtgtatgc gcgttagctc gctgcgtgga gaaagatatc gacgtggcgg
      721 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgcc acttcgccaa
      781 tgcacatcgc caccaagctg cgcagcacgc tggacgaggt gatcgaacgc gctatctata
      841 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
      901 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
      961 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatta
     1021 gcggcctgta tgaacgcgtg cctaacatcg acaaagccat tatttccgta catacccacg
     1081 acgatttggg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     1141 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttccctg gaagaagtca
     1201 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     1261 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     1321 aagccattgt tggcagcggc gcattcgcac actcctccgg tatccaccag gatggcgtgc
     1381 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     1441 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     1501 ggtataaaga aagtgaatat aatttagaca atttgtacga cgccttcctc aagctggcgg
     1561 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     1621 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     1681 tcgccaccgc cgccgtcaaa ctggcctgcg gcgaagaagt caaagcagaa gccgccaacg
     1741 gtaacggtcc ggtcgatgcc gtctatcagg cgattaaccg catcactgac tataacgtcg
     1801 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     1861 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     1921 tcgagtcctc cgccaaagcc atggtgcacg tacttaacaa tatctggcgt gccacagaag
     1981 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     2041 gtcgaagaat taccatattg ccgtattgcc gggggatggc attggtccgg aagtgatgac
     2101 ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt gcgatgcgca tcactaccag
     2161 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     2221 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaaatg
     2281 ggaacattta ccaccggacc agcaaccaga acgcggcgcg ctgttgcctt tgcgtaagca
     2341 cttcaaatta ttcagcaacc tgcgtccggc aaaactgtat caggggctgg aagcattctg
     2401 tccgctgcgt gcagacattg ccgcaaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     2461 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     2521 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     2581 atctgctcgt aaacgtcgcc ataaagtgac gtcgatcgat aaagccaacg tgctgcaatc
     2641 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atatcgaact
     2701 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     2761 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     2821 tggctcgatg gggatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     2881 accggcgggc ggctcggcac cagatatcgc aggtaaaaac atcgccaacc cgattgcgca
     2941 aattctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     3001 cattgaacgc gccattaacc gcgcattaga agaaggcatt cgcaccgggg atttagcccg
     3061 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     3121 aggggtgtaa tcatggctaa gacgttatac gaaaaattat tcgacgctca cgtagtgtac
     3181 gaagccgaaa atgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     3241 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     3301 accttcgcca ccatggatca caacgtctct acccagacta aagacattaa tgcctgcggt
     3361 gaaatggcgc gcatccagat gcaggagctg atcaaaaact gcaaagaatt tggcgtcgaa
     3421 ctgtatgacc tgaatcaccc ctatcaaggg atcgtccacg taatggggcc ggaacagggc
     3481 gtgaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     3541 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacgcaa
     3601 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     3661 ggcattaccg caaaagatat cgtgttggca attatcggta aaaccggtag cgcaggcggc
     3721 accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg atttaagcat ggaaggtcgg
     3781 atgaccctgt gcaatatggc aatcgaaatg ggcgcgaaag ccggtctggt tgcacctgac
     3841 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     3901 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     3961 gtcactttgc aggcagaaga gatttcaccg caggtcacct ggggaactaa cccaggccag
     4021 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgc
     4081 gcgtcggcag aaaaagcgtt ggcctatatg gggctgaaac cgggtattct gctgaccgaa
     4141 gtggctatcg acaaagtgtt tatcggttcc tgcaccaact cacgtattga agatttacgc
     4201 gcggcggcgg aaatcgccaa agggcggaaa gtcgcgccag gcgtacaggc gctggtggtt
     4261 cccggctctg gtccggtaaa agcgcaggcg gaagcagaag gtctggataa aatctttatt
     4321 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     4381 cgtctgaatc cgggcgaacg ttgtgcctcc accagcaacc gtaactttga aggccgtcag
     4441 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgccgc ggctgtgacc
     4501 ggccatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     4561 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     4621 cccgaaacag tttttgcaga aagtgacccg tactggtttt ggcgcgcatc tgtttaacga
     4681 ctggcgtttt ctggatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttctc
     4741 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     4801 tgagcacgcg ccctgggcat tgaccgacta cggttttaag gtggtgattg cgccgagttt
     4861 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
     4921 cgcagaagtg gatgagctgt ttgcgctggt gaaagccaat ccggggattc attttgatgt
     4981 agacctggaa gcgcaagagg tgaaagcggg tgagaaaacc tatcgcttta ccattgatgc
     5041 cttccgccgc cactgcatga tgaacggtct ggacagcatt gggcttacct tgcagcacga
     5101 cgacgccatt gcggcttatg aagcgaagca acccgcgttt atgcgttagt ttgtacaccg
     5161 tccgatactc aatcgccaaa aagaaaaaag ccagcagata atcactgctg gcgtggcgat
     5221 taacaccaat actcagtcac acatgatgtc cgcaaatcag tagtcagaat gactcccctc
     5281 ccactgcatc agttcttcaa gcatggttaa tcgttgtggg acgcttaacc aacgcaacca
     5341 gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac tgacgaatgg ggcacctcct
     5401 tgcttcatcg ttaggaatta ttgacttaat atagggaaaa taaaattgct gtcttttgcg
     5461 caggagttcc ccttttatgc catctgctcg tctgcaacaa cagttcatcc gcctgtggca
     5521 atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa ctggcagcgt tattgagctg
     5581 ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag gatcgcggct ggctgacgtg
     5641 ggaagcggaa gtcgggcgtg gtaaacgctc gcgtctgaca ttcctctata ccgggctggc
     5701 gcttcagcaa cagcgggcgg aagacctgct ggagcaggac cgtatcgacc aactagtgca
     5761 gttagttggc gacaaagcga ctgtgaggca aatgctggtt tctcatctgg gccgcagctt
     5821 ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt ccgttgcgta atctgctacc
     5881 tggcagcgca ttgcgccgtt ccgaaaccca tatcgcccgg caaatcttca gttcgctaac
     5941 gcgcataaat gaggaaaatg gggaactgga agcagacatc gcccaccact ggcagcaaat
     6001 atcaccgctt cactggcgtt tctttttgcg tccaggagtc cattttcatc atggtcgtga
     6061 actggaaatg gacgacgtga tcgcctcttt aaaacgaatc aatacgctgc cgctctattc
     6121 gcatattgct gacattgtgt cgccgacgcc ctggacgctg gatatccacc tcacgcagcc
     6181 ggatcgctgg ttgccgttac tgctgggaca agttccggcg atgatcctgc cgcgcgaatg
     6241 ggaaaccctc agtaactttg ccagccatcc catcggcacc ggtccgtatg cggtgattcg
     6301 caacagcacc aatcaactga aaattcaggc attcgatgac ttcttcggtt accgggcatt
     6361 aatcgatgaa gttaacgtct gggttctgcc ggaaattgcc gacgagccag ccggagggct
     6421 gatgctaaaa ggtccacagg gcgaggaaaa agagattgaa agccgcctgg aggaaggttg
     6481 ctactattta ctgttcgata gccgcaccca tcgcggggcg aatcagcaag tcagggactg
     6541 ggtaagctat gtgctttctc caactaatct ggtctatttc gctgaggaac agtaccagca
     6601 actgtggttc ccggcttatg gactgctccc ccgttggcat catgcccgca ccataacgag
     6661 cgaaaaaccg gctggtctgg aaagcctcac cctgaccttt tatcaggatc acagtgagca
     6721 tcgggtgatt gccgggatca tgcagcagat tctggcaagc caccaggtca cactggaaat
     6781 caaagagatc agctacgatc agtggcatga aggagagatc gagagcgata tctggcttaa
     6841 cagcgccaac tttacgctgc cgctggattt ttcgctgttc gcgcacctgt gcgaggtacc
     6901 gctgctccaa cactgtattc caatcgactg gcaagtcgac gccgcccgct ggcgcaatgg
     6961 cgaaatgaac ctggcgaact ggtgccaaca actggtcgcc agcaaagcaa tggtgccact
     7021 tatccaccac tggctgatca ttcagggaca acgcagtatg cgcggcctgc gcatgaacac
     7081 cctcggctgg tttgatttta aatcagcgtg gtttgcgccg ccggatccag agtagcttgc
     7141 gccttttggc ggtcatgagc gcatttttta tattcagacg gaatgataaa atacattact
     7201 tttatatttt ctctgtttta tgcatcgtct gattagcgtc gcaaataaga ttaaagaagg
     7261 ggcattccag cccctctttc atattctgag ttaatcaata acggcgcagt ctagccttgt
     7321 tctggccgga aaggtcagct ttttcaggtt aacctcgcgc aggtcagtca taccaacggc
     7381 tattgttcgt ttcagcattg ctcaggtcaa cctcgagaca gtcaatacca caaatgacaa
     7441 ccccgttaaa gtaagcatta ctcagatcaa tccccccggt cctggtgctc tttttcagct
     7501 gcaactccag agtaaatatc gcctcatcgc caataaaaaa actgacgcat aacgtacctt
     7561 ctggcgagag tgaggccttc gcggccatat tttccggatc ttttgcgtat tctttgagtt
     7621 cattaaattt tatcagccag tgagttaact tatcacgcgc cttcccgtcg acagccccgg
     7681 taaagtattt tgagtagtca tcactattct ggaaataaaa tgcatccttc agaatttgcc
     7741 ttgccgcagt ctgggaatgt ttatctgact ggaagagatt gcgtaacgtc tcccatagct
     7801 cttcaaagat attcatattc tcggtctttt tgaccaagtt agccatcctg ataatctgag
     7861 aattagtaag gcctggatct ttagtggtat tactagcagg gatactaatc tgttctgggt
     7921 ttcttacttt catgctgata gctccactct gaataaaaaa ctcagaataa gcgtcctgaa
     7981 taagaatact gtcaaaaatc gctcattcac gcaataaacg gttacgttac ctattttgac
     8041 tgtagcgaag ggattctttc ggatcaacgc tctccagcta tgcaaatacg ctcttgatag
     8101 ctgaacgaat attggctttt ttaatacgcc ttctctatgg attgctggtt gataacaaaa
     8161 tcactacact aacgccgttc tcaacggggt gccacgcgga cgcgtgcgct gagaaaatac
     8221 ccgtcgaacc tgatccggat aacgccggcg aagggatttg aggctccttc tcaagtcctt
     8281 tgccactctt ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct gttgctgtgc
     8341 acagcgcccg ttttcgctaa acccgttctg actgtttata cctacgattc cttcgccgcc
     8401 gactgggggc ctggtccggt ggttaaaaaa gcctttgaag ccgactgtaa ttgcgaactg
     8461 aaactggtgg cgctggaaga tggcgtttcg cttctcaacc gtctacggat ggaaggcaaa
     8521 aacagtaaag ccgatgtggt gctggggctg gataacaatc tgttagacgc cgccagtaaa
     8581 accggactgt ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc cggcggctgg
     8641 aataatgaca ttttcgtacc gtttgattat ggctacttcg ccttcgttta tgacaagaac
     8701 aaactgaaaa acccgccaca aagcctgaaa gaactggttg agagcgatca aaactggcgg
     8761 gtgatttatc aggatccgcg caccagtaca ccggggctgg gtctgttgct atggatgcaa
     8821 aaagtctatg gcgatgacgc cccacaagcc tggcagaaac tggcgaagaa aacggtcacg
     8881 gtcaccaaag gctggagcga agcctacggc ctgtttttaa aaggtgaaag cgatctggta
     8941 ctgagttaca ccacctctcc ggcttatcac attctcgaag agaagaaaga taactacgcc
     9001 gccgcgaact tcagcgaagg tcactatctg caagtggaag tcgccgctcg caccgctgcc
     9061 agcaagcagc cggagctggc gcaaaaattc ctccagttta tggtttctcc ggctttccag
     9121 aatgcgatcc caaccggcaa ctggatgtat ccggtggcaa acgtcacgct gcctgccggt
     9181 tttgaaaaat tgaccaaacc cgcaaccacg ctggagttca cgccagccga agtggcggca
     9241 caacgtcagg catggattag cgaatggcaa cgcgccgtca gccgttaatt cccggctggt
     9301 taattccagg tgtaagcgcc gccacgctgg tggtagcggt tgcgctggcg gcgtttctcg
     9361 ccctgtggtg gaacgcgccg cagggtgact ggtcggcagt ctggcaggac agctacctgt
     9421 ggcatgtggt gcgcttctcc ttctggcagg cgtttctctc ggcgctactc tctgtcgtac
     9481 ccgcgatatt cctcgcccgc gcgctctatc gcaggcgctt tccgggtcgg ctggcgctgt
     9541 tgcgtctgtg cgcaatgacc ttgatcctcc cggtgctggt tgctgttttc ggcattctta
     9601 gcgtctatgg tcgccagggc tggctggcat cgctctgcca atcgctcggt ctggagtgga
     9661 ccttttcgcc ctacggcctg caaggcattt tgctggccca tgtgtttttt aatctgccga
     9721 tggcgagccg cttattactc caggcactgg aaaacattcc cggcgaacaa cgtcagcttg
     9781 ccgcccagct tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg ccgtggttac
     9841 ggcgacaaat cccgccggtt accgcgctta tctttatgct ctgtttcgcc agcttcgcca
     9901 ccgtgttatc gctgggcggc ggtccgcagg caaccactat cgagctggca atctatcagg
     9961 cgctgagtta cgactacgat cctgcccgcg cggcgatgct ggcgctgatc cagatggtgt
    10021 gttgccttgg gctggtgctg ttgagtcagc gactgagtaa ggccattgcg cccggcacca
    10081 cgctgctgca aggctggcgc gacccggacg atcgtctgca tagccgcatt tgcgacacga
    10141 tgttaattgt gctggcgctg ctgctgttgc tgccaccgtt gctggcggtg atcgtcgatg
    10201 gggtaaatcg ccagttgccg gaagtgctgg cacaaccggt gctgtggcag gcgctgtgga
    10261 cctcgttgcg tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc atgatgctgc
    10321 tatggagcag tcgcgaactg cgggcgcggc aaaaaatgct ggcgggccag gcgctggaga
    10381 tgagcggcat gttgatcctc gctatgccgg ggattgtgct ggcaacgggc ttctttttac
    10441 tgctcaacaa cactatcggc ctgccacaat ctgctgacgg cattgtgatt ttcaccaatg
    10501 cgttaatggc gatcccctac gccctgaaag tgctggaaaa cccgatgcgc gatatcaccg
    10561 cccgctacag catgttatgt cagtcgctgg ggattgaagg ctggtcacgc ttaaaagtag
    10621 tggagctgcg cgccctgaaa cgcccgctgg cgcaggcgct ggcctttgcc tgcgtgctgt
    10681 cgattggtga ttttggcgtg gtggcgttgt tcggtaacga tgatttccgc accctgccgt
    10741 tttatctcta ccagcaaatt ggctcctatc gcagccagga cggcgcggtc accgcgttaa
    10801 ttctgctgct gctctgtttt ctgctgttta gcgtgattga aaaaataccg gggcgaaatg
    10861 ttaaaactga ctgatatcac ctggctttat caccatttgc cgatgcgttt tagcttaacg
    10921 gtagaacgcg gcgagcaggt ggcgatcctc gggccaagcg gcgcgggtaa aagtaccctg
    10981 ctgaacttga tcgccggttt tctgacacca gccagcggtg cgctgactat cgacggcgtt
    11041 gatcacacca cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca ggagaacaac
    11101 ctgttcagcc acctgacggt cgcgcagaac atcgggctgg ggcttaatcc aggattgaag
    11161 ctgaacgcgg tacagcagga gaaaatgcac gcgattgccc gtcagatggg gattgataat
    11221 ttaatggcgc ggttaccggg cgagctttcc ggcggtcagc gacagcgcgt ggcgttagcg
    11281 cgttgtctgg tacgcgaaca gccgatttta ttgctcgatg aaccgttctc tgcgctcgat
    11341 ccggcgctac gtcaggagat gttgacgctg gtgagcacta gctgccagca gcaaaaaatg
    11401 acgctattga tggtgtcgca cagtgtggaa gatgcggcgc ggattgccac gcgctcggta
    11461 gtagtcgccg acgggcgaat cgcctggcag ggtatgacca atgagttgtt gagcggtaag
    11521 gcgagtgctt cggcgctgtt gggaattaca ggttaatatg cactgtcgga tgcggcgtga
    11581 acgccttatc cgacctacgg gtgcgttttg tccgcatcaa gcgccccaca caagactatg
    11641 ccctaaactc caaccacttt acgcaaaata tcgatataca ccggcattaa cggatggcga
    11701 actaacacca ccagcgccac cacgccgatg gcagaaatca acggcgtcag ccacaacaaa
    11761 cgaccgcggg acaaataatg actcaaacga tcggttgctt tgccgctgcg ccataaccgc
    11821 cagcacagcc agccaccaac ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac
    11881 tcaccgctct gcattccggc tggaatatcg atcgccgcac ccgccagaat ccccggcagg
    11941 aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtgataaa ttttgccacc
    12001 gggagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc aacaaaacga
    12061 ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt atcgagtaat
    12121 gctttgttct tcttcagaaa tgaccaacga tgcaatggct ttttaaaacg ccagcccagc
    12181 cagaaggaga tccagtcgcc cagcagacaa ccgacgatcc ctgccagcca ggcgtgccag
    12241 aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac cgtaccgggt
    12301 agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac cgccatcaat
    12361 gaatacacgg tggattgggt aataaagtgt tccagcaatg cttgcataat gtgcctgtca
    12421 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
    12481 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
    12541 acggaactcg ctcgggctgg ccccggtgca ttttttaaat actcgcgaga aatagagttg
    12601 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtag tgctcaaaag
    12661 cagcttcgcc tggctgatgc gttggtcctc gcgccagctt aagacgctaa tccctaactg
    12721 ctggcggaaa agatgtgaca gacgcgacgg cgacaggcaa acatgctgtg cgacgctggc
    12781 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
    12841 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
    12901 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
    12961 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaacc
    13021 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
    13081 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
    13141 tcctggcggg aacagcaaaa tatcacccgg tcggcagaca aattctcgtc cctgattttt
    13201 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
    13261 gtcgataaaa aaatcgagat aaccattggc ctcaatcggc gttaaacccg ccaccagatg
    13321 ggcgttaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
    13381 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagatatt gccgtcactg
    13441 cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta aaagcattct
    13501 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
    13561 cagaaaagtc cacattgata atttgcacgg cgtcacactt tgctatgcca tagcattttt
    13621 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
    13681 atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc gattttggca
    13741 gtgattctgt gcgagctttg gcggtggact gcaccaccgg tgaagaaatc gccaccagcg
    13801 tagagtggta tccccgttgg caaaaagggc aattttgtga tgccccgaat aaccagttcc
    13861 gtcatcatcc gcgtgactac attgagtcaa tggaagcggc actgaaaacc gtgcttgcag
    13921 aacttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacagt accggctcga
    13981 cgcccgcacc gattgatgcc gacggtaacg tgctggcgct gcgcccggag tttgccgaaa
    14041 acccgaacgc gatgttcgta ttgtggaaag accacactgc ggttgaagaa gcggaagaga
    14101 ttacccgttt gtgccacgcg ccgggcaatg ttgactactc ccgctatatt ggcggtattt
    14161 attccagcga atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg
    14221 cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta
    14281 ccacgcgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataag tcgctatggc
    14341 acgaaagctg gggcggcctg ccgccagcca gtttctttga tgagctggat ccgatcctca
    14401 atcgccattt gccttccccg ctgttcactg atacctggac tgccgatatt ccggtgggca
    14461 ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg
    14521 gcgcgtttga ctgccatatg ggcgcagttg gtgcaggcgc acagcctaac gcactggtaa
    14581 aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc
    14641 gggcagtgaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc
    14701 tggaagcagg ccaatcggcg tttggtgata tctacgcctg gttcggtcgc gtactcggct
    14761 ggccgctgga acagcttgcc gcccagcatc cggaactaaa agagcaaatc gacgccagcc
    14821 agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc
    14881 tgccggtggt gctcgactgg tttaacggcc gccgcacacc gaacgctaac caacgcctga
    14941 aaggggtgat taccgatctt aacctcgcta ccgacgctcc gctgctgttc ggcggtttga
    15001 ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg
    15061 ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg
    15121 cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccaa tgctgtgcgc
    15181 tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccatcagccc
    15241 agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa caggcacaac
    15301 gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc
    15361 ttccaacttc cgccccggca caggctgccc aggccgttcc gactctataa ggacacgata
    15421 atgacgattt ttgataatta tgaagtgtgg tttgtaattg gcagccagca tctgtatggc
    15481 ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgtgaaagc gctgaatacg
    15541 gaagcgaaac tgccctgcaa actggtactg aaaccgctgg gcaccacgcc ggatgaaatc
    15601 accgctattt gccgtgatgc taattacgac gatcgttgcg ctggtctggt ggtgtggcta
    15661 cacaccttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg
    15721 ctgcaattcc acacccagtt caacgcggcg ctgccgtggg acagcatcga tatggacttt
    15781 atgaacctga accagaccgc gcacggcggt cgcgagttcg gcttcattgg cgcgcgtatg
    15841 cgtcagcaac atgccgtggt taccggtcac tggcaggata aacaagccca tgagcgtatc
    15901 ggctcctgga tgcgtcaggc ggtatctaaa caggataccc gtcatctgaa agtctgccga
    15961 tttggcgata acatgcgtga agtggcggtc accgatggcg ataaagttgc cgcacagatc
    16021 aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt ggtgaactcc
    16081 atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg
    16141 cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag
    16201 ctggggatga aacgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa
    16261 gatttgcacg gtctgaaaca gcttcctggt ctggccgtac agcgtctgat gcagcagggt
    16321 tacggctttg cgggcgaagg cgactggaaa actgccgccc tgcttcgcat catgaaggtg
    16381 atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta tcacttcgag
    16441 aaaggcaatg acctggtgct cggctcccat atgctggaag tctgcccgtc gatcgccgca
    16501 gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc
    16561 cgcctgatct tcaataccca aaccggtcca gcgattgtcg ccagcttgat tgatctcggc
    16621 gatcgttacc gtctactggt taactgcatc gacacggtga aaacaccgca ctccctgccg
    16681 aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg atctgccaac tgcttccgaa
    16741 gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac
    16801 gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc
    16861 cgcctgccag cgtttaaaga cgcgctgcgc tggaacgaag tgtattacgg gtttcgccgc
    16921 taagtagccg catccggtat gtaacgcctg atgcgacgct ggcgcgtctt atcaggccta
    16981 cacgctgcaa ttttgtaggc cggataagca acgcgcatcc ggcattcagc gcctgatgcg
    17041 acgctgacgc gtcttatctg gcctacacgc tgcgattttg taggccggat aagcaacgcg
    17101 catccggcac gaaggagtca acatgttaga agatctcaaa cgtcaggtat tagaagccaa
    17161 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    17221 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagca tcatgaccgc
    17281 tgacgatatg gtcgtggtca gcatcgaaac cggtgaagtg gttgaaggtg cgaaaaagcc
    17341 ctcctccgat acgccaactc accgactact ctatcaggca ttcccgtcca ttggcggcat
    17401 tgtgcacacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc
    17461 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    17521 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    17581 cgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt ctcacggccc
    17641 atttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    17701 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    17761 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    17821 actgtataaa accacagcca atcaaatgaa accaggctat aatcaagcct ggttttttga
    17881 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggataccccg
    17941 caaggaacag aggtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    18001 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    18061 ttgcggggtg aacaaggctt tcgcctgacg ccgctggcgt taaaggattt tcaccgccag
    18121 ccggtgtatg gcctttactg tcgcgcccat cgccagttga tgaattacga aaagcgcctg
    18181 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    18241 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cgctatcgtt
    18301 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctattgat
    18361 attgaaacca cccgccacgg agagctgtac tgcatcggcc tggagggctg cgggcagcgc
    18421 atcgtttata tgctgggacc agagaatggc gacgcctccg cgctcgattt tgaactggag
    18481 tacgtcgcca gccgcccgca gctactggaa aaactcaacg cctggtttgc aaactacgat
    18541 cctgatgtga tcatcggttg gaacgtggtg cagtttgatc tgcgaatgct gcaaaaacat
    18601 gccgagcgtt atcgtattcc gctgcggctg gggcgcgata acagtgagct ggagtggcgc
    18661 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    18721 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    18781 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    18841 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    18901 gagctggtga cgcagatctt ccataaaact gaaatcatgc cgtttttgct cgaacgagca
    18961 acggtgaacg gcctgccggt ggatcgacac ggcgggtcgg tggcagcgtt tggtcatctc
    19021 tattttcccc gtatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    19081 cacgccagcc ctggcggcta cgtgatggat tcgcgacctg ggctttatga ttcggtgctg
    19141 gtgctggact acaaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    19201 gggctggtgg aaggcatggc gcagcctgat ccagagcaca gcaccgaagg ctttctcgat
    19261 gcctggttct cgcgagaaaa acattgcctg ccggagattg tgactaacat ctggcacgga
    19321 cgcgatgaag ccaaacgcca gggtaacaaa ccgctgtcgc aggcgctgaa aatcatcatg
    19381 aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcggctggta
    19441 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcgtt gattgaagcg
    19501 cagggctacg acgtgatcta cggcgatacc gactcaacgt ttgtctggct gaaaggcgca
    19561 cattcggaag aagaagcgac gaaaatcggt cgtgcactgg tgcagcacgt taacgtctgg
    19621 tgggcagaaa cgctgcaaaa acaacagctg accagcgcac tggaactgga gtatgaaacc
    19681 catttctgcc gttttctgat gccaaccatt cgcggagccg ataccggcag taaaaagcgt
    19741 tatgccgggc tgattcagga gggcgacaaa cagcggatgg tgtttaaagg gctggaaacc
    19801 gtgcgtacgg actggacgcc gctggcccag cagtttcagc aggagcttta cctgcgtatc
    19861 ttccgcaacg agccatatca ggagtacttc cgcgaaacca tcgacaaact gatggcgggt
    19921 gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag
    19981 cgtaatgttc cgcctcatgt acgcgccgct cgtcttgccg atgaagaaaa ccaaaagcgc
    20041 ggtcgcccct tgcaatatca gaaccgcggc accattaagt acgtatggac caccaacggc
    20101 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    20161 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    20221 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    20281 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    20341 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    20401 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    20461 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    20521 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    20581 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    20641 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    20701 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgcc tgtttgccgg
    20761 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    20821 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcacca
    20881 gctcaacatc gctcatgatg ttggccgccg ccacgcgccg cgcgtcctgc tggctgacga
    20941 agtgggttta gggaaaacca ttgaagccgg gatgatcctt catcagcaac tgctctctgg
    21001 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    21061 aatgctgcgc cgtttcaacc tgcgctttgc gctgtttgat gatgagcgtt atgccgaagc
    21121 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    21181 tgcccgccgt agcaaacagc gtctggaaca tctctgtgaa gccgagtggg acctgctggt
    21241 ggtcgatgaa gcgcatcacc tggtgtggag cgaagacgcg ccgagccgcg aatatcaggc
    21301 tattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcaa ccccggaaca
    21361 gctgggaatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    21421 tttcgcgcaa ttcgttgaag agcagaaaaa ttatcgtcca gtagcggacg ccgttgccat
    21481 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgcttggcg agatgatcgg
    21541 cgagcaggat atcgagccgc tgttgcaagc agcaaacagc gacagcgaag atgcccagag
    21601 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    21661 ccgtaacacc cgtaacggtg tgaaaggctt cccgaaacgc gagctgcaca ccattaagct
    21721 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    21781 aagtgcggaa gaccgcgccc gcgatatgct ctacccggag cgtatttatc aggaatttga
    21841 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    21901 gaccagccat cgctctcaga aagtgctggt gatctgcgct aaagctgcca ctgcgctgca
    21961 actggagcaa gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    22021 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    22081 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    22141 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    22201 tcgtatcggc caagcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    22261 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    22321 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    22381 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    22441 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    22501 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    22561 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    22621 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    22681 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcgc agtttattac
    22741 ctgggaacat ccgctgatcc gcaacggtct ggatctgatt ctttctggcg ataccggtag
    22801 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    22861 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    22921 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    22981 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    23041 cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    23101 tgcccgtgca ttgattgatg cagcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    23161 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    23221 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    23281 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    23341 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    23401 gtcaacaagc cgagcggttt gttgtctgtg ccgggtcgtc tggaagagca caaagacagc
    23461 gtgatgacgc gcattcagcg agattatccg caggcagaat cggtgcatcg tctggatatg
    23521 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    23581 cagttccgcg agcgcgtgcc gaaaaagcag tatgtgaccc gcgtctgggg gcatccatcc
    23641 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    23701 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    23761 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    23821 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    23881 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    23941 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaaccttt cctgtacgtt
    24001 tataccctca tcctaagggc tgcctcttgt acataaatcc ccgagtccgg ggattttttg
    24061 tttctgccaa actgctttca gttgttgcgt gtggctacgc tttccattcg gtaattccct
    24121 tcctgctcca cagcgaattt ttgaactttt tcttcacccg ttgaccgtct cttatatttc
    24181 attttatcgg gtctgttatg gtcctttctg attgttattc atgggctaat tagcagtctg
    24241 gttggcgatg cccatctcgg tgatccgcgt cgtacacgac gccttgcgtc actagcctct
    24301 tctctagctc atcatgcagg tctttctaat tgtgaaatca tcccacgcta ctgctaaggt
    24361 tgaaggggcg ttccagcccc ttcaacctta gcttattctg gctcattcaa taattgcgtt
    24421 gtcagtaatt tgttcagcag tactttgttt agtagcaact tcttcagtat caattttttt
    24481 gggggtgatt ttattaataa taattacctc atcatatagg tgggtagcac cgtccacggt
    24541 agcaccgtcc agggtagcac cgtccaggtt agcattccac ccaggttcca agtaaaggac
    24601 tttattgtga cctttcattg ttttgagacc aaggtaacaa tccctcatgt caacgccggg
    24661 aaggtaagcc tctctaagat caacgcccaa gttacctggg cctttatgca gctgcaacgt
    24721 ctgagtacat atctccttat tttcaataaa aaaagtgaca cttaactcga tatcaccgaa
    24781 ggtctgtgcg acccagctca tattgccctt tgcgaccatc tcatccggat tttttgcgca
    24841 tgttttgagt tcattgaata attccagact atggataaac ttttctttct cattctcgtt
    24901 gacagcccca gtaaagtatt ttgaggcgtt gatgttgcca gcctttttat tcacgaaatt
    24961 gaacagaagt ttataagcct cagtctgttt atctgaccag aacagatgac gtaacgtctc
    25021 ccacaagcgt tgaaagatat tcatcttcat gtccatcttg tccccgctca ccatcttata
    25081 aatatcgtta ttgctcatat ttaaaagtgt agccggcatg cctggaactg atactttcat
    25141 ggtaatagcc tcgcattgag ttaaaaactc atgatgcgcg ctctgaataa atatactttc
    25201 aaaaatcgct cattcagata tgtgtttttt atttttaaga cgtaaaaata taattccgct
    25261 cgccgtaaag ctctcaacct taagcagggt caacgcgata aaaccaacag aaaccctccc
    25321 gtaaaccgta tattgagcct gtccaccatt ctgagtaaac gccttttctc aaagaagtga
    25381 ccgccagggc ggccaccttt tatcgtgggc agttactcag catcatttac agggtcattt
    25441 aaaccctttc tgctgcttaa tcagctcata ggcctgctga atttcctgcg ctttctgctt
    25501 cgccatctcc atcatctcag gcggcaaacc tttcgccacc agcttatcgg gatggtgttc
    25561 actcatcagc ttacggtagg cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc
    25621 cagcacatta caggcatctt ccagcgttgg gccacgctgc gcttgctgcc agttaccacc
    25681 gccagattgc tgctgataac cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa
    25741 ctggtcaaac tgggcgcggg agatccctaa ttcttcagca atgacataca gcaccgcccg
    25801 ttcattcggg tgcagtgaac catcagcaaa cgccgcctga atctggatct ccagaaacat
    25861 acgaattaag tcaaaacgac caaagcagac actgcgaaac tggcgcatct tttcgcgcag
    25921 cgggtaattg tctgattttc ccacccggaa cgcattttgc gccgcagtac gggaagcgcc
    25981 atgaagattc attcggtcca tcaactggct ggcgatatga atatcagcct ccgtgacgcg
    26041 acctttggat ttggttaaat gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg
    26101 acgctcacgc tggttggcga accacgccat tttacggcta cgggctttat caaacatatg
    26161 gccaattaac aggcctaaca ctacgcccca aaagccgccg cccatcagta aggccacggc
    26221 cacgccaatg atttttcccc aatactgcat atattcccca aatcgacaca cggatatcag
    26281 ggctatctcc cacaatataa aggtgctttt accgttttcc ggctgcggtc aattgtggga
    26341 catcgcctat aatttgcatt atcatacctg tcattcaatg ccgtgcctaa caccactgac
    26401 gctattcgga caggattaac actagcgtag agatgacgag tacgttagtc tctgaccgtt
    26461 tgtcacgcgc aacgttaccg atgatggaac aataaaatca acgtatgaaa aaacgtatcc
    26521 ccactctcct ggccaccatg attgccaccg ccctttatag tcaacaggga ctggcagccg
    26581 acctcgcctc acagtgcatg ttgggcgtgc caagctatga ccgtcctctg gtacagggcg
    26641 ataccaatga cttacccgtg actatcaatg ctaaccacgc gaaaggggac tacccggatg
    26701 acgccgtgtt tactggcagc gtggatatca tgcagggtaa cagccgtctg caggccgacg
    26761 aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga gccggtacgt accgttgatg
    26821 cgctcggtaa tgtccattac gacgataacc aggtgatcct caaagggccg aaaggctggg
    26881 cgaatctgaa caccaaagat accaacgtct gggaaggtga ttaccagatg gtgggtcgcc
    26941 agggtcgcgg taaagcggac ctgatgaaac aacgtggcga aaaccgctat accattctgg
    27001 ataacggtag ctttacctcc tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg
    27061 aaattattca tgaccgcgaa gaacaagttg cggagatctg gaacgcccgc tttaaggtgg
    27121 gtccggtatc gatcttttat agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct
    27181 ctggtttctt gatcccgaac gccaagtaca ccaccaccaa ctactttgag ttctacctgc
    27241 catattactg gaacatcgcg ccaaatatgg atgccaccat cacgccgcat tatatgcatc
    27301 gtcgtggcaa catcatgtgg gagaacgaat tccgctacct ctcccaggcg ggcgctggct
    27361 tgatggaact ggactatctg ccttcagata aagtctatga agatgaacac ccgaacgatg
    27421 acagttcacg tcgttggtta ttctactgga accactccgg ggtcatggat caggtgtggc
    27481 gtttcaacgt cgactacacc aaggtcagcg atcctagcta cttcaatgat ttcgataaca
    27541 agtacggttc cagtactgac ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc
    27601 aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt tttcagcgaa cagaacacca
    27661 gtagctactc ggcagagccg cagttagacg ttaattacta ccagaatgat gttggtccgt
    27721 ttgatacgcg tatttacggc caggcagtgc actttgttaa caccagagac gacatgcctg
    27781 aagcaacccg tgttcacctg gaaccgacca tcaatttgcc gctctctaat aactggggca
    27841 gcatcaatac cgaagcgaag ttgctggcaa cccattatca gcaaaccaat cttgactggt
    27901 ataactccag aaacacgacc aagctggacg aatccgttaa ccgcgtaatg ccgcaattca
    27961 aagttgacgg caaaatggtc tttgaacgcg atatggaaat gctggctccg ggttataccc
    28021 aaacgctgga accgcgcgcg cagtatttgt acgtgccgta tcgcgatcag agcgacatct
    28081 ataactacga ctcgtctctg ctgcaatctg actactctgg cctgttccgg gaccggactt
    28141 acggcggtct tgaccgtatt gcctccgcta accaggtgac gaccggtgtc acatctcgca
    28201 tatatgatga tgctgccgtt gaacgtttta atatttccgt tggtcaaatc tactatttca
    28261 cggagtctcg cactggcgat gacaacataa catgggagaa tgacgacaaa acgggttcac
    28321 tggtgtgggc aggcgatact tactggcgta tctccgagcg ttggggattg cgtggcggga
    28381 ttcagtacga tacacgtctg gataacgtag cgaccagtaa ctccagcatt gaataccgtc
    28441 gggatgaaga ccgtctggta cagctgaatt accgttacgc cagcccggaa tatattcagg
    28501 ctacgctgcc taagtactat tccactgctg agcaatataa gaatggtatt tcgcaggtag
    28561 gtgctgtcgc cagctggcca attgccgatc gttggtccat tgttggggcc tactactacg
    28621 acaccaatgc gaacaagcaa gccgactcta tgttaggtgt gcaatacagc tcctgctgct
    28681 atgcaattcg cgtcggttac gagcggaagc tgaacggttg ggataacgat aaacaacatg
    28741 cggtatatga caacgcaatc ggctttaaca tcgaacttcg cggcctgagc tccaactacg
    28801 gtctgggtac gcaagagatg ctgcgttcga acattctgcc gtatcaaaac actttgtgat
    28861 ttgttgattt accacgtaat ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac
    28921 tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc tgccccccag
    28981 gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat
    29041 ggattaatgc agtcggtaaa actgaacgct gctcaggcaa ggcagcaact tcctgatgac
    29101 gcgacgctgc gccaccaaat catggaacgt ttgatcatgg atcaaatcat cctgcagatg
    29161 gggcagaaaa tgggagtgaa aatctccgat gagcagctgg atcaggcgat tgctaacatc
    29221 gcgaaacaga acaacatgac gctggatcag atgcgcagcc gtctggctta cgatggtctg
    29281 aactacaaca cctatcgtaa ccagatccgc aaagagatga ttatctctga agtgcgtaac
    29341 aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct ggcgcagcag
    29401 gtgggtaacc aaaacgacgc cagcactgag ctgaacctga gccacatcct gatcccgctg
    29461 ccggaaaacc cgacctctga tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc
    29521 gatcaggcgc gtaacggcgc tgatttcggt aagctggcga ttgctcattc tgccgaccag
    29581 caggcgctga acggcggcca gatgggctgg ggccgtattc aggagttgcc cgggatcttc
    29641 gcccaggcat taagcaccgc gaagaaaggc gacattgttg gcccgattcg ttccggcgtt
    29701 ggcttccata tactgaaagt taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc
    29761 gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga tcatgactga cgaacaggct
    29821 cgtgtgaaac tggaacagat tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc
    29881 gcagcgaaag agttctctca ggatccaggc tctgctaacc agggcggtga tctcggctgg
    29941 gctacaccag atattttcga tccggccttc cgtgacgccc tgacccgcct gaacaaaggt
    30001 caaatgagtg caccggttca ctcttcattc ggctggcatt taatcgaact gctggatacc
    30061 cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg cataccgcat gctgatgaac
    30121 cgtaagttct cggaagaagc agcaagctgg atgcaggaac aacgtgccag cgcctacgtt
    30181 aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg
    30241 ccgggattgg cccggactta gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg
    30301 ttgtttgtgc cgatgccact ctccttaccg accgggcagc gatgctcggt ttgccgctca
    30361 ccctccgccc ttattccccc aactcccctg cacaaccgca aactacgggc acattaacgc
    30421 tacttcctgt cgcgctacgt gaatctgtca ctgcggggca gttagcgatt gaaaatgggc
    30481 attacgtggt ggagacgctg gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg
    30541 cgctgatcac aggccccgtg cataaaggcg tcattaacga cgcaggcatt ccgtttaccg
    30601 gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga
    30661 ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat atcgcagatg
    30721 ctatcacccc tgcacttttg cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa
    30781 aatttggtat tgccgaaccg cgcattctgg tctgcgggct gaatccgcac gcgggcgaag
    30841 gcggtcatat gggtacggaa gagatagaca ccattattcc tgtgctcgac gagctgcggg
    30901 cgcaggggat gaaactcaac gggccgctgc ctgccgatac cctgtttcag ccgaaatatc
    30961 tcgataacgc cgacgccgtg ctggcgatgt accacgatca gggtcttccc gtgctaaaat
    31021 accagggctt cgggcgcggt gtgaacatta cgctgggcct gccctttatt cgcacatcag
    31081 tggaccacgg caccgcactt gaactggcgg gacgtggcga agccgatgtc ggcagtttta
    31141 ttacggcgct taatctcgcc atcaaaatga ttgttaacac ccaatgaata atcgagtcca
    31201 ccagggccac ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat
    31261 cgacagcatt gtttctgcca ttaacccgca aaagggccag gcaatggtcg aaatcggccc
    31321 cggtctggcg gcattgaccg agccggtcgg cgaacgtctg gaccagctga cggtcatcga
    31381 acttgaccgc gatctggcgg cacgtctgca aacgcatcca ttcttaggcc cgaaactgac
    31441 gatttatcag caggatgcga tgacctttaa ctttggcgaa ctggccgaga aaatgggtca
    31501 gccgctgcgt gttttcggca acctgcctta taacatctcc acgccgttga tgttccatct
    31561 gtttagctat actgatgcca ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa
    31621 tcgtctggtt gcaggaccga acagcaaagc gtatggtcga ttaagcgtca tggcgcaata
    31681 ctattgcaac gtgatcccgg tactggaagt tccgccgtca gcctttacac caccacccaa
    31741 agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg atgcctcacc cggttaaaga
    31801 tgttcgtgtg ttgagccgca tcaccaccga agcctttaac cagcgtcgta aaaccattcg
    31861 taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg ggaatgggga tcgacccggc
    31921 gatgcgagcg gaaaatatct ctgtcgcgca atattgccag atggcgaact atctggcgga
    31981 gaacgcgcct tttcaggaga gttaacgatg atcaattcgc cccgagtgtg tattcaggtt
    32041 caaagcgtct acattgaggc acaatcttca cctgataatg aacgttacgt ttttgcttat
    32101 accgtaacca tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg ttactggctg
    32161 atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag
    32221 ccacttatcg cgcctggcga agagtaccag tacaccagcg gtgcaatcat tgaaaccccg
    32281 ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc
    32341 gacattcccg tattccgact cgccgttccc acactcattc attaaaagaa tatggcgaca
    32401 taccttattg gcgacgttca tggttgttac gatgaactga tcgcattgct gcataaagta
    32461 gaatttaccc ctgggaaaga taccctctgg ctgacgggcg atctggtcgc gcgcggcccg
    32521 ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg
    32581 ggcaatcacg atctgcatct gctggcagta tttgccggga tcagccgcaa taaaccgaaa
    32641 gatcgcctga caccgctgct ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt
    32701 cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg tgatggccca cgcagggatc
    32761 acgccgcagt gggatctgca gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgctg
    32821 tcgagtgact cctatccctt ctttctcgac gctatgtacg gcgatatgcc aaataactgg
    32881 tcaccggaat tgcgggggct gggaagactg cggtttatca ccaacgcctt tacccgtatg
    32941 cgtttttgct tcccgaacgg tcaactggat atgtacagca aagaatcgcc ggaagaggcc
    33001 cctgccccac tgaaaccgtg gtttgcgatt cctggacctg tcgctgaaga atacagcatc
    33061 gcctttggtc actgggcatc gctggaaggc aaaggtacgc cggaaggtat ttacgcgctg
    33121 gataccggct gctgctgggg tgggtcatta acctgcctgc gctgggaaga taaacagtat
    33181 tttgtccagc cgtcgaaccg gcataaggat ttgggtgagg gagaggcggt cgcgtcttaa
    33241 atcccgtcga gcataaaatc taatgctcct ttaatcaacg ttcggtgcgc gctggcatcg
    33301 caaaatttcg ttcccagcgc gttaactgga acagttgcag tctcatgcgt catgagcaca
    33361 tattctttgc cgtctaccag taaaaggatt gggttaagcc gttctggtgg gcgaatacgg
    33421 ctaaatcgtt caataggtgt taatggaata acaatacggc gattaatcac accaataatg
    33481 tcgctggtaa catcgataac aaagggaaac gcggcgttcc tgctgcgact gcggtatacc
    33541 gtaaattgca tcgctagaag ctccggtact catcagagaa acatccggtt tcatcagcca
    33601 attgatttaa cgcagcgata gcttcagcgt tctcttcacg ccaacgtgtt gctgcatgtt
    33661 ttttaagttc agcattgagc gcaaccgtaa gggtggcgct catatttaag cctgcatcac
    33721 gagcgcgatt gactaactca cggtcgacgg tgacggtcac actttgtgtg gtacgttttg
    33781 cagtcatgat atcgtgctcc tgtacaattt atacgtctag tatacacgga tatctgcacg
    33841 tatcagtgat gccggaattc taatatacaa aattaccgcc gctccagaat ctcaaaacaa
    33901 tagctgtgag agttctgcgc atcagcgtcg tggaattcgc tgaataccga ttcccagtca
    33961 tccggctcgt aatccggaaa atgggtgtcg ccttccactt ctgcgtcgat atgcgtcagg
    34021 tacagttttt gcgctttcgg caggaactgc tcataaacgc gaccgccgcc aatcaccatg
    34081 atttctggta cgtcaccaca cgccgcgatg gcttcatcca ccgacttcac ccacgttacg
    34141 cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg caacggacga
    34201 ccgatggatt ccctggtatg gcggcccata attacgggtt tatttaaggt gttgcgttta
    34261 aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc gataacgcga
    34321 tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa aaaattgtcg
    34381 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    34441 ttatattctg ctggcgagtc cacgctctct ccctggactc gccgcattac aatgaaacaa
    34501 aaacaaacag ttagctgtaa agtgtgattt acgtcactct ttattaggat gagggtttcg
    34561 tttccggttc atccgccatg ttgccggtat gtttaccttc ttctgttccc tgccagccat
    34621 gtcgttgaat taatgacaga tgttcgcggt cctcggtgat gatctcactt aacatcgcgc
    34681 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt
    34741 ccaccatctg aatattaaag cggcggaaca catcggcacg ttctcgcgct tcatacggcc
    34801 ccagacctaa actttccagc gccagacgcc cggttttcag cgcaccttca aaggtttcac
    34861 gctctggctt ttcaacgcct gcctgacgca aacaaatgta gtgatcgaca tcgcgggcgc
    34921 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    34981 tttgcggatc gtcaatggcg ttaatcagca cttccgcttt cgccgctccc gcagattcca
    35041 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcagcgttt
    35101 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    35161 gtccggtgat ctgcccgaaa cgaccgaatc cggcaatgat tacccgcggc tgttcttcgt
    35221 cgatctcatc ggcttcacgc gcctcttcag tagaagattg ctcaaggcga ttgaggatca
    35281 ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc cagggtcagc gatttcgccc
    35341 actccggctc cagcacattc gccatctgcg ccgcgccaaa taccacaaag gcaaactcac
    35401 tgccctgccc taacaacacc gcaaaccagc gacgctgttt atttggcacc tgcaacggtc
    35461 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    35521 tgcgcaatgg gttttcaatc agcgtgccaa agtctatcga catgccaaca ccgatgaaaa
    35581 acagccccaa cagcaaacct ttaaagggtt cgatatcgct ttccagcgca tgacggtatt
    35641 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    35701 cttccagcag caaaccaaag ccaaacacaa ggaataacgc cacggcacta aacacttccc
    35761 gcaagccaga gcgggcaaca aaacgcagcg ccggacgcgt gacatagcgc cccagcagta
    35821 ccaccagcac cagcgcgccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    35881 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatt gccgcgatat
    35941 cctggaacag cagcaccgca aaggcactgc ggcccatttg cgtcaccatc agattgcgtt
    36001 cattcatcgc ctgcatggca atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt
    36061 ccgcgacctg ccagcgcagc ccaagtaaca tacagaacag ccccagcagg ccgccgcaaa
    36121 tcaccatctg caatgcgcca ccgccaaaca ctgccgcacg cagcttccac agcctttgtg
    36181 gatcgagttc gaggccgata ataaacagca tcagcaccac gccaatctcg gcaaagtgca
    36241 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    36301 tcaggtagcc aagtaccgat cccagaccaa gacgtactgc aatgggtaca atcagcgccg
    36361 ccgaaccgag ataaatcagc gcctgaacca gcgtatggct atccatgatg ggcctcctgc
    36421 cattccagca gacgttgctt atagtgacgc gcctgccctt cgagggtttc gtcgtcacaa
    36481 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    36541 gcctgtagcg gctgcgacag cacatcaaag cccggatgcg caccaatttc aaaatggctt
    36601 tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg
    36661 tgaccgtaag cccagccgtg cgagaagact ttatcgatcc aaagtttaag gagcggagga
    36721 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga cagcgcctcc
    36781 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    36841 acgccttcca gcgtccttgc ctgttcaaac atccgtttat tcgcatggga atgatgcgga
    36901 tacggatgcg cataaattat aagaatcata ggtagcccgc gtgtttacgc ctttgttttc
    36961 ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa taatattgat tcagaaaaga
    37021 gataaatctg aacaaattac cccattctct gcccgcggat cgtcatattg ctggtctgcg
    37081 ccagtgacat ccctcgagtc tccggggcaa acgctacgga aatcaacaag ccaaacagcg
    37141 agatacccgc ccccattagc atcgtgttac tgataccgta attattgata aagatcggta
    37201 gtgcccaggt cgaaacaatg gtgccaatac gacttaagga cataatcacg cccacggcag
    37261 aggcgcggat atccgtcggg aagagttcat taggatagag ccactgcaaa ttacccggcc
    37321 cgccagagaa aaaggcatac accgcaaagg ccatcactac cagccagatc cccatatccg
    37381 ggatcagccc caaaaccgcc agcgccagcg tcatcatggc aaagctgcca atcaacaatg
    37441 gacgccgtcc ggcagtgttt agccacagca tcggcggaat acagccgagc ataaagaaca
    37501 ggctaatcac cacgttcccc agtgccgcgt ttttgccaac acccaatccc aacaaaccaa
    37561 cgatttgcgg gccaaaggtg taaatggcga acattgggat cacctggcag gtccagatgg
    37621 cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt
    37681 gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagtttgatc atcatctctt
    37741 cgcactcttt tactcgccct ttgcgtaata accagcgggg agattcaggc agttcgaatc
    37801 gaccaatcaa aatcaacaaa caggggatcg ccgcgctacc cagcatccag cgccagccgc
    37861 cttccacatc ataaagccag tagccgacca gatcggcaca ggtcgcgccg acataccaca
    37921 tggcggcgat aaagctgatg gaaaacgccc gctgacgggt actggagaac tcagtgatca
    37981 tcgaagtggc gatgggatag tctgcaccga tgacaatgcc gataagtacc cgcatcacca
    38041 acagttcgac gggggatgaa acaaacatcg tcgccaccga tatcacgccg atggcgatga
    38101 tatcaatgag gaacattttg cgccgtccga ctttatcgga aatataacca aacagcgatg
    38161 tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa gccaatccag tcagcgtcca
    38221 gttttagcgc cggcgtcagt tgctccagcg ccacgccaat cattaccagt atataaccat
    38281 ccagaaacgg accaccgctt ccccacagca aaatgcggcg gtgaatagag gaaaatttaa
    38341 gatcgtcaaa gtttctggac ggttgcatgg gttattcctg ttttttgcaa tgagagtcag
    38401 tgcggcgtta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    38461 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    38521 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    38581 cggggcacgc tttcaccagc agctccagcg cctgtttatc ggcatcggct ttcacaacaa
    38641 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    38701 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc
    38761 cactttcttg ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat
    38821 ggtgaacagg tcacgcgcca cgcctaccgc cagttccggg tagccgctaa acatgcgtgg
    38881 gttatcaagg aaggccggta gtttctggta catacgcata tcgcgcagcg ggccactctc
    38941 aagatgctga cgatattccg cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    39001 aagcacggtt tttgctgcag cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    39061 aaaaccgagg ttcatacaca ttccggcggc atcaccggca atcaatacgc cgtcaccgac
    39121 cagctccggc agcatgttga tgcctgcttc cggcactacg tgagcggaat attccaccag
    39181 cttgccaccc gcgatcagcg gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg
    39241 cggcaccgat tttttcgcgt catgcagatg atgcagacca caaaccagcc ccagcgacag
    39301 ggtgttttca ttggtataaa gaaaaccgcc gcccatcagg ccatcggtgg gtgatcccgc
    39361 aaataggcaa gccgccccct gattaccctg caactgaaaa cggtcttcaa taaccgactt
    39421 cggtaactcg atcagttcct tcacgccaac cgccacattc gtcggtttaa cgcgttttgc
    39481 catccccagc ttttcggcaa ggatggagtt cactccatca gcaaggatca ccgttttcgc
    39541 ttcaatcaca tcgccatcgg cttctacacc gacgactttg ccatcgcgct gtacgaggtt
    39601 atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct tcggcctgct ccatcagcca
    39661 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggcgagg tttcgtcacc
    39721 gttgcagtag tccatagtca tcgctgactt ttccgtcata aacgcgagtt tttcatgggt
    39781 gatcaggcgt tctaccgggg cggattcggc gaaaccagga ataatgtgtt ccaggctgtg
    39841 ggcatagaga cgcccgccgg tgacgttctt ggcacctgcg gaattgccac gctcgataac
    39901 taacacttgc gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagccctgc
    39961 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcagga
    40021 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg
    40081 atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    40141 tctttattga tggcgaaaat ggtttgcgac gcgttagcgc caaccatgtg ctggatctgc
    40201 ccggagatcc ccacagctag gtacagttca ggtttcagca tcaggttgga gataccgaca
    40261 tagcgttcgt gctccatcca tttttcgttt tctgccaccg gacgagaaca ggccaactcc
    40321 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    40381 cgaccgacgc tgaccaccag gcgggctttg tcgagatcga cgctgttgct ctggcgcgcc
    40441 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    40501 ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc
    40561 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac caccgtaaac catatgtttc
    40621 actgttgctt taccgtcctg tacgctgacg gtgctggcat cgttagacac cgccgcttta
    40681 aggcgataac ccagttttgc cgccagtaat ttgccgcgac gggtgtttgg caacagcacc
    40741 aggccgtctg caccgtgctg gcgaatagtg tcagccatga cactggcgta atcttcgatc
    40801 atccgatcgt ccggtttgcc gtttaatttc cagacatgat tagcgccgag ctggattgcc
    40861 tgtgcgccgt cggcatcatt gaggacaaag gcattgattt gattagctaa agcctgcgca
    40921 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    40981 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    41041 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ctcgcgctgt ttcggcgcgg
    41101 caacctgttg ttctgaccat gctgcctctg cgttaaaacc gatatccgcc gccgaccata
    41161 cctggactgg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    41221 tgatatcagt ggaaacagca acaaccgcag gcagtggaat gcttaaggtt tcggtttcat
    41281 cttccagttc gcgctcaacg gtgagggtat ctgccgtcag ggagataatt ttgctgacgc
    41341 cgttaactgc cggaatattg aggatttcgc ctaccagcaa accaacctgc tgggcataaa
    41401 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctgg gcggctgcag
    41461 ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    41521 tcagttcatc cggaccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    41581 taccgcccac acttagggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    41641 ccgcttcaat agcgttgaga tcgtattggc ttattttggc atcggcttta ctgaagtcta
    41701 acgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    41761 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    41821 taattacaga tatcatgccc tttaatttgg aaataaaact ttaatcacca atattgaaaa
    41881 tgtcacacgc agataaatcc aactttcaat attgttaagt tcctcaccaa tattgaaaac
    41941 acggcgtagc aaaaagaaat tttcaata
//
LOCUS       DBFIFS010000017        41404 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.017, whole genome shotgun sequence.
ACCESSION   DBFIFS010000017 DBFIFS010000000
VERSION     DBFIFS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.017"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(72..848)
                     /gene="sseB"
                     /locus_tag="N7A07_000857"
     CDS             complement(72..848)
                     /gene="sseB"
                     /locus_tag="N7A07_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU2475578.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(991..2274)
                     /gene="pepB"
                     /locus_tag="N7A07_000858"
     CDS             complement(991..2274)
                     /gene="pepB"
                     /locus_tag="N7A07_000858"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU2475579.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2452..2652)
                     /gene="iscX"
                     /locus_tag="N7A07_000859"
     CDS             complement(2452..2652)
                     /gene="iscX"
                     /locus_tag="N7A07_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU2475580.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2664..2999)
                     /gene="fdx"
                     /locus_tag="N7A07_000860"
     CDS             complement(2664..2999)
                     /gene="fdx"
                     /locus_tag="N7A07_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU2475581.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3001..4851)
                     /gene="hscA"
                     /locus_tag="N7A07_000861"
     CDS             complement(3001..4851)
                     /gene="hscA"
                     /locus_tag="N7A07_000861"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU2475582.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(4868..5383)
                     /gene="hscB"
                     /locus_tag="N7A07_000862"
     CDS             complement(4868..5383)
                     /gene="hscB"
                     /locus_tag="N7A07_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU2475583.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5479..5802)
                     /gene="iscA"
                     /locus_tag="N7A07_000863"
     CDS             complement(5479..5802)
                     /gene="iscA"
                     /locus_tag="N7A07_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU2475584.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(5819..6205)
                     /gene="iscU"
                     /locus_tag="N7A07_000864"
     CDS             complement(5819..6205)
                     /gene="iscU"
                     /locus_tag="N7A07_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU2475585.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6233..7447)
                     /gene="iscS"
                     /locus_tag="N7A07_000865"
     CDS             complement(6233..7447)
                     /gene="iscS"
                     /locus_tag="N7A07_000865"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU2475586.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7559..8047)
                     /gene="iscR"
                     /locus_tag="N7A07_000866"
     CDS             complement(7559..8047)
                     /gene="iscR"
                     /locus_tag="N7A07_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU2475587.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8348..9085)
                     /gene="trmJ"
                     /locus_tag="N7A07_000867"
     CDS             complement(8348..9085)
                     /gene="trmJ"
                     /locus_tag="N7A07_000867"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU2475588.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            9204..10007
                     /gene="suhB"
                     /locus_tag="N7A07_000868"
     CDS             9204..10007
                     /gene="suhB"
                     /locus_tag="N7A07_000868"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU2475589.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10152..11006
                     /locus_tag="N7A07_000869"
     CDS             10152..11006
                     /locus_tag="N7A07_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2475590.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            11197..12477
                     /gene="csiE"
                     /locus_tag="N7A07_000870"
     CDS             11197..12477
                     /gene="csiE"
                     /locus_tag="N7A07_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU2475591.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12469..13608)
                     /locus_tag="N7A07_000871"
     CDS             complement(12469..13608)
                     /locus_tag="N7A07_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU2475592.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13768..14658)
                     /gene="hcaR"
                     /locus_tag="N7A07_000872"
     CDS             complement(13768..14658)
                     /gene="hcaR"
                     /locus_tag="N7A07_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU2475593.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            14794..16155
                     /gene="hcaE"
                     /locus_tag="N7A07_000873"
     CDS             14794..16155
                     /gene="hcaE"
                     /locus_tag="N7A07_000873"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU2475594.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16152..16670
                     /gene="hcaF"
                     /locus_tag="N7A07_000874"
     CDS             16152..16670
                     /gene="hcaF"
                     /locus_tag="N7A07_000874"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU2475595.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16670..16990
                     /locus_tag="N7A07_000875"
     CDS             16670..16990
                     /locus_tag="N7A07_000875"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU2475596.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            16987..17799
                     /gene="hcaB"
                     /locus_tag="N7A07_000876"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             16987..17799
                     /gene="hcaB"
                     /locus_tag="N7A07_000876"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU2475597.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            17809..19011
                     /gene="hcaD"
                     /locus_tag="N7A07_000877"
     CDS             17809..19011
                     /gene="hcaD"
                     /locus_tag="N7A07_000877"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU2475598.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            19108..19530
                     /locus_tag="N7A07_000878"
     CDS             19108..19530
                     /locus_tag="N7A07_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU2475599.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19578..20450)
                     /locus_tag="N7A07_000879"
     CDS             complement(19578..20450)
                     /locus_tag="N7A07_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU2475600.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20462..21556)
                     /locus_tag="N7A07_000880"
                     /pseudo
     CDS             complement(20462..21556)
                     /locus_tag="N7A07_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(21589..22587)
                     /locus_tag="N7A07_000881"
     CDS             complement(21589..22587)
                     /locus_tag="N7A07_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2475601.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22612..24123)
                     /locus_tag="N7A07_000882"
     CDS             complement(22612..24123)
                     /locus_tag="N7A07_000882"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2475602.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(24146..25129)
                     /locus_tag="N7A07_000883"
     CDS             complement(24146..25129)
                     /locus_tag="N7A07_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU2475603.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25226..28507)
                     /locus_tag="N7A07_000884"
     CDS             complement(25226..28507)
                     /locus_tag="N7A07_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU2475604.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            28625..29818
                     /locus_tag="N7A07_000885"
     CDS             28625..29818
                     /locus_tag="N7A07_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU2475605.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(29881..31134)
                     /gene="glyA"
                     /locus_tag="N7A07_000886"
     CDS             complement(29881..31134)
                     /gene="glyA"
                     /locus_tag="N7A07_000886"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU2475606.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31462..32652
                     /gene="hmpA"
                     /locus_tag="N7A07_000887"
     CDS             31462..32652
                     /gene="hmpA"
                     /locus_tag="N7A07_000887"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU2475607.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(32697..33035)
                     /gene="glnB"
                     /locus_tag="N7A07_000888"
     CDS             complement(32697..33035)
                     /gene="glnB"
                     /locus_tag="N7A07_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU2475608.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33096..34430)
                     /gene="glrR"
                     /locus_tag="N7A07_000889"
     CDS             complement(33096..34430)
                     /gene="glrR"
                     /locus_tag="N7A07_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU2475609.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34420..35133)
                     /gene="qseG"
                     /locus_tag="N7A07_000890"
     CDS             complement(34420..35133)
                     /gene="qseG"
                     /locus_tag="N7A07_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU2475610.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35298..36725)
                     /gene="qseE"
                     /locus_tag="N7A07_000891"
     CDS             complement(35298..36725)
                     /gene="qseE"
                     /locus_tag="N7A07_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU2475611.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37301..41188)
                     /gene="purL"
                     /locus_tag="N7A07_000892"
                     /gene_synonym="purI"
     CDS             complement(37301..41188)
                     /gene="purL"
                     /locus_tag="N7A07_000892"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU2475612.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         9700 a        11506 c        10487 g         9711 t
ORIGIN      
        1 caatttactg gtgattaaca accttcagag cagcaggtaa gcccgaatgc cgccctttgg
       61 ggcggcatat tttagattat ccgattctgt ttaaagtcac gcaaaaaacc accccagcga
      121 cgttcataga atggcgcaat atgttcggta ataaagtggc taattccttt ttcccctttt
      181 ttcacctgac aaatatcgat tggttcatcg ccaggtaatg tatcggtcgc tacacttccc
      241 gccacctgaa taatttcttc gatatcacca tcggcttcaa tgccaataag taaattaggc
      301 tgtgcctctt cgttctcttt aattgaacaa ataaaagcac gcttcacagg cttaatggtt
      361 ttaaataagg tggtgagtga atcaatcatt tgtgctggcg gctctgcgac ttccgataat
      421 atcagcgatt caccgccttc caggatttcc tggctgctca gcggatttcc ctcttcacca
      481 atcaacaaac tgatttcacg cggcataaat tctttaccgg tcggcagttt ggcattaagg
      541 aagagcgttt cgccaagggt catctcaaac aatgtgcgaa cgggcattac gacaaatgcc
      601 tgttcgtctt caaccgcctg ttgaagtgct tctaacgagg taaaaaaagg aatgacgctg
      661 gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcgc tatcttcaac cacagcctcg
      721 ccctgcgccg ccgtaccagg cacccagacg gtggattcca gtagagtacg gaaaaaggcc
      781 gggcggtgcg ccggttcggt tgctgctttt tccagcaggt cttcaagttc attttttgtt
      841 tcggacataa gaaccacaat tcattcaacg ttcggggcac aagtgcctga tgcgccatac
      901 ttatcaggcc tacaagggat tcacaatttg ttgaatttgc aatactttgt aggccggata
      961 aggcgtttac gccgcatccg gcatattagt ttacgccgtt aacagattag caatcgtgcg
     1021 cacaccaagt cccgtagcac ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc
     1081 cgagcagtcg atatgcagcc agccttgctg atagttctca acaaagtgcg acaggaagcc
     1141 cgctgccgtg ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca gttcggcaaa
     1201 gttagacggc agctggctgc ggtggaattc cgccagcggc agacgccaga atggttcgtt
     1261 ctcttgtgag gcactcgcca gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc
     1321 gtgataatca ttacccagcg cagttttcgc cgccccggtg agggtcgccg catcaatgat
     1381 catttccggt ttctgcgcac tggcatcaat cagaccatcg gcaagcacca ggcgcccttc
     1441 ggcatcagtg ttcatcactt caactttttt accgttgcga taagtgatga tatcgcccag
     1501 cttgaacgca ttgccgctaa tcaggttatc cgcacagcag aggaacagct tcacgcgctt
     1561 gttcagtccg cgcgtaatgg caaatgccag cgccccggta accgttgccg cgccgcccat
     1621 gtccgacttc atcgagtcca taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa
     1681 agtgatacct ttacctacca ggcacgcgta cactggcgct tctttatcgc cagttgggtt
     1741 gtaatccagc gccagcaata ccggagaacg ttctgaaccg cgtccgacgg tgtgcagccc
     1801 catataacct tgctcacgca gatcttcgcc tttggtgatc cgataagtca cacgatcgct
     1861 cgcgacgttg ctgatcagat caacagcacg ctgtgccagt tgcgatggtc ccaattcttc
     1921 tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt tatccagttc
     1981 ctggcgctgg gcatcgtcca gatccggcca ctccacttta cgcgtgcctt tcggggcttt
     2041 gtaaccttgc cagaatgccc agcatcgatc cgcatcccag ccttcaccgc ttaactgaac
     2101 atgcttgatg cccagaccgt caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc
     2161 tgccccgttc aaatgcaggg taatgccgtc attattaatg ctgtaagttg ctttttctcc
     2221 ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc atcgcttctg tcattttagt
     2281 tatccttctt tgtaaaaggg ccgccagttg gcagccccag gatttttccg ctaaatgatt
     2341 cgcgttgcag gaaggcggca agtgagtgaa gccccaggag catagataac tatgtgactg
     2401 gggtgaacga gcgcagccaa cgcatctgcg gcgtgaagca tgacgcggaa attattcggc
     2461 ctcgtccagc cagactaaca aaatcgcttc gaggattttc tcgttggatg cctgcgggtc
     2521 gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt
     2581 cggatcaaga tcgggatacg catcgtacag tgcttcgcca atttcgcggc tatcggtcca
     2641 cttaagtccc atactaacct ctgttaatgc tcacgcgcat ggttgatcgt gtaacgcggg
     2701 atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc agcttaaacg gctttccggc
     2761 tccagtcccc aggctttgtc cagcatgtcg tcttcctgct ctgagctttc tggaagcgag
     2821 tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag cacaggattt ttcacaggcg
     2881 tgttcaatct cgataccgtt acgcagagct gcgtcgagaa tggtttcacc gctattagct
     2941 tccagaacag cgccatcagg gcagagatcc tgatgaggca aaataacaat ctttggcata
     3001 ttaaacctcg tccacggaat ggcctttcag cgcacgacga accgactggt ccatgcggcg
     3061 agcggcgaaa tcctgggttt gtttgtctac gtttttaatc gcttgttcga tggcgtcaac
     3121 gtcatcgccc tgcgccactt cactcaggtg agcggcagca tcgtcaatga cctgacgttc
     3181 tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg tgcagacttt ccagcacacg
     3241 cgccgcttct actttttgtt ctgccagcat tcgggctttt acgtcctgct cggcatagct
     3301 cattgagtct ttgatcatcg aagcgatttc gctatcggtc agaccgtaag acggtttgac
     3361 ctgaatagac gcctcaacgc cggtggattt ctccatcgcc gtcacgctca aaagaccgtc
     3421 ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca gccggtagcg ccggaatacc
     3481 acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc agttcgcgct caccctgcat
     3541 tacatggata gacatcgccg tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc
     3601 cactggaata gtggtattac gcggaatcac tttctccacc aggccgccca tcgtttcgag
     3661 gcccagcgac agtgggatca catcaagcaa cagcatttcg ctgtctggct tgttacccac
     3721 cagaatatcc gcctgaatcg ccgcgccaat agcgacgact ttatccgggt cgatggaagt
     3781 cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc accagcggca cgcgagtaga
     3841 accgcccacc atcaccactt ccagcacttc atcagcttct acacccgcgt ctttcagcgc
     3901 gcgacgacaa gccaataagg ttcgttttac cagtggcgcg atcagttcat tgaattgttc
     3961 acggctgatt tcgccctgcc agcccgcaac gttaacggtc acggagtccg catcgctcag
     4021 cgcgattttg gctgcaatgg cggcatccag cagttcacgc tgaacgcggt tatcgctacg
     4081 atcaggaatg cccgcctgct cgcgaatgta atccgccagc agatggtcga aatcatcgcc
     4141 gccgagcgcg gaatcaccgc cggttgccag cacttcaaac acgccgcgac ttaagcgcag
     4201 aatggaaata tcaaacgtcc cgccaccgag gtcataaacg gcgatcacgc cttcctgacc
     4261 ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg ttaagtaagc gcaggacgtg
     4321 aaggcccgcc agacgcgccg cgtctttggt gccctgacgc tgggcatcgt caaagtacgc
     4381 cggaacggtg ataactacac catccagctc gcctgccagg gcttcagttg cccgcgccgc
     4441 cagtgctttg aggatgtccg cagaaacgcg caccgggttc agcagccccg ccgccgtttc
     4501 aatcatcggc aggccgtttt cgctggcctg gaattgataa ggcagatgcg gatagcgttg
     4561 ctggatatca gccagcgagc gtcccatcag gcgtttaaca gaactaattg tgttggcggt
     4621 atcgagcgct gcattagtac gcgcgtcata acccaccgaa tgcccttgct gttgatagtg
     4681 aacaacagat ggcagcaggt gacggccttc atggtcggct aacgtttcgg cctgaccgct
     4741 gcgcactgtc gccaccagcg agttggttgt gcccaggtca ataccggccg ccagacgacg
     4801 ctgatgcggc gcagcactca aaccaggttc actaatttgt aataaggcca tgtttagctt
     4861 ccagaaatta aaaatcgagc agtttttctt cgagttgttc ggcactgctt cgcagtttat
     4921 cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc ccaggtctcg ttgtctaact
     4981 gttcaaccat caactgatgg cgggtatcaa acatcttttt cacacgtttg ataaagcttt
     5041 ccagccgcgc ttcatctttc gcctgttcga tctcgtccag ctcttcgcgc agctccaact
     5101 gttccatcag gaacgcggtg tcgcgcacag tatgctgctc gctggcgaga tcaaagccgt
     5161 gcaaagaaag caaatattcc gcgcgcatta acggatgacg cagcgtttgc caggcctgat
     5221 taatggttgc agattgctgt acggcggcga gttgttccgc ctggcttccg ctggcgaatt
     5281 tatcaggatg atactgacgt tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga
     5341 gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc cataacattc tcagggctgc
     5401 gatccgccgc gataacggcg aatcaggtaa aacaaccccc acgcgcaggc gaccacggtg
     5461 gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg caaccacact catctttaac
     5521 gttcgggttg gtgaatttaa acccttcgtt caggccttct tttacgaagt ccagctgcgt
     5581 accgtccaga aattgcaggc ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa
     5641 cacgatgtct tccggcgtcg gttcgtcaac aaattccagt acataagcca tacctgaaca
     5701 tccggaggtt ctcacgccca gacgtaggcc aaaccctttg ccacggttag ccagaaaggt
     5761 atttactcgc gctgctgcac tgtcgctaag tgtaatcgac ataaccaaac ctcaactctt
     5821 attttgcttc acgtttgctt ttatagtccg caatggcggc tttgatcgcg tcttctgcca
     5881 gaatagaaca gtgaattttc accggcggca gttcaagttc ttcagcaata tcggtgtttt
     5941 tgatagcctg cgcttcgtcg agagacttcc ctttcaccca ttcggtgacc agggagctgg
     6001 aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat
     6061 cgttgacttt aatctgcaac ttcattacgt cgccacaggc cggtgcaccc accatgccgc
     6121 tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt
     6181 cgataacttt ttcgctgtaa gccattataa attctcctga ttccgatacc gattaatgat
     6241 gagcccattc gatgctgttc agatccacgc cctgcttgta catttcccac agcggagaaa
     6301 ggtcacgcag acgaccgatg gatttacgaa ctaactcgat ggtgtagtcg atctcttctt
     6361 cagtagtaaa acgacctaaa gagaaacgga tagagctatg tgccagctcg tcgttcagcc
     6421 ccagcgcgcg cagcacgtag gacggttcga ggcttgctga cgtacaggcg gaaccggaag
     6481 aaaccgcgag atctttcagc gccataatca gcgactcacc ttcaacgtag ttgaagctga
     6541 cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat
     6601 ctttgatgcc gttccacaga cggttacgca ggccgcgcag acgttccatc tcggtcgcca
     6661 tctcttcttt tgcgatgcga taggcttcgc ccattccgac gatctggtga acaggcagag
     6721 tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc
     6781 gcggtttacg acgcacatac agcgcaccga tacctttcgg gccatagatt ttgtgaccgg
     6841 agaaagacat caggtcaact ttcaactggc tcaggtcgat aggcagtttg cccacgctct
     6901 gggttgcatc aacgtgatag ataatgccac gagcacggca catttcgccg atagccgcga
     6961 tatcctgcac cacgccgatt tcgttattta cgtgcatgat agaaacaagg atggtgtcgt
     7021 cacgcatcgc tgcttcgagt tctttcaggt cgataatacc gttacgctgc ggtgcgaggt
     7081 aggtgacttc gaaaccttcg cgctccagct gacggcaggt atccaatacc gctttgtgtt
     7141 cggttttgct ggtgattatg tgcttgcctt ttttctgata aaagttggct gcacctttga
     7201 tcgccaggtt gtcagactcg gttgcaccag aggtaaagac gatttcacgc ggatcagcgc
     7261 cgaccagatc ggcaatctga ttacgggcga tatctaccgc ttcttcagcc tgccagccga
     7321 aacggtgaga acgggaggcc gggttaccaa aggttccgtc catcgtcata aactgcatca
     7381 ttttctcggc aacacgcggg tccaccggcg tggttgcgga gtagtcgaga taaatcggta
     7441 atttcattgc tctataaact ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg
     7501 atgtacgacc gtgtttacgg agcgcgatac gccccggcct gattctgaat actttttatt
     7561 aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat
     7621 gctgacgacc agacacatcc agcacttcct ggttgttaac cagttcgcct aaagtaatgt
     7681 tattgagaaa accggtaaga cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt
     7741 tatcgccgcc ctggcagccg cctttaccct gacaacgggt ggcatctaca gattcgtcaa
     7801 cggcgctaat cacttcgcca acggcgatgc tgctggcatc tttgcctaac agataaccac
     7861 cgcctggtcc acgtacgctg gaaaccagac catttttacg cagacgagaa aacagttgtt
     7921 ccagataaga aagggaaatt ccctgacgtt cggaaatatc agccaacggt accgggcccg
     7981 cttcagagtt gagcgcaacg tcaagcattg cggtcacggc atagcgccct ttagatgtca
     8041 gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg
     8101 ggggtattgc atagcagggt caagtctgac attcccgagt aaattggtca actatttact
     8161 tgactgattt agtcgggtat ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc
     8221 tgttatcctt ccagaccatg ggcgggttga gggcaattat tcgcattagc gcaggcctgt
     8281 tcccctcacc ctaaccctct ccccgaaggg gcgaggggac cgtccgtgca cttatctggc
     8341 ttagccctca ggccttatta cctttattct gctgctctat agaagccaga atcccgcgca
     8401 ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca
     8461 tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat
     8521 gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct
     8581 gctcgccatt ttcctgagtt gccagccagg ccatacgcac ttcataggca ataacctgaa
     8641 ccgccatcgc caggttcagc gagctgtatt cagggttagc ggcaatcgcg acatgataat
     8701 gacacttctg caactcttca ttggtcaggc cgacacgttc gcgaccaaac accagcgcca
     8761 ccggggtatt tgccgcttca gcaacgcttt tcagaccgca ttcgcgcggg tcgagcatcg
     8821 gccacggcag cgtgcgggaa cgtgcgctgg tgcccaccac caggctacaa ccggctaacg
     8881 cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc cccgccgcca
     8941 gggcaatcgc ctgggagtca ggtttcacca gtggattaac cagccacaga ttggttaatc
     9001 ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca
     9061 gcacaattcg aatattttgc agcattgtct ttcttcgtct aaagattatt cgcgcatctt
     9121 atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct
     9181 ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc
     9241 gcaaggcggg taatttgatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc
     9301 agaaaggcag taacgacttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg
     9361 acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg
     9421 aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggcact accaacttta
     9481 tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag
     9541 ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg
     9601 cacagctgaa cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac ggtactattc
     9661 tggcgaccgg cttccccttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg
     9721 gcaaactgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg
     9781 cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgc ccgtgggact
     9841 tcgctgcagg cgagctgctt gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg
     9901 gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccgcgtgtt gttaaagcca
     9961 tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg
    10021 tgatatcact cacccgccct cgcctttcag gcgctattcc gaaatacttc ctcaccgctt
    10081 tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc
    10141 aaggaaaggt tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct
    10201 ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc
    10261 ctgacgataa aatttacggt cccgacccct ggtcggcgga atctgtcgaa tttacggcta
    10321 aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg
    10381 ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc
    10441 tggtcagttg gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg
    10501 gtaaatcaaa aggcacgccg tcccaggccg gattgctgga cgatacgcaa agcgccatca
    10561 atgtggtgcg ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta
    10621 ttggcggggc gaatattctt gctgttattg gtcagggtga tcgtgaaggc atacgtgcag
    10681 tgatcctcga ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca
    10741 gtggctactt acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga
    10801 tcccgctttt actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa
    10861 agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca
    10921 ttgatgcttt ttccgatcgt cacggcgacg tttatcgcga acagatggtg aactttatcc
    10981 ttagcgcgtt gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa
    11041 tcaccttaag acatgctaat ccactggtca ggacagttta agatgagaaa aattctgtga
    11101 cgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag caaactttag
    11161 ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca
    11221 tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg
    11281 ttgaccgcca ccatggcaac cttcagcgag cttaatggtg tggatgatga tattgccagt
    11341 cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac actgacgact
    11401 ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt
    11461 cactggctac gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca attcaccccc
    11521 gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat
    11581 ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga atcgcgcgat
    11641 attcatttcc tgtgtctgta tctgcaatat tgtttgctgc agcaccacgc tggaattacg
    11701 cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg
    11761 caggaaattg gacgccactg gcagcgtcgg gcgctccagc ctgtaccacc tgatgagcca
    11821 ctgtttatgg cactactttt ttccatgttg cgggttcccg atccattgcg ggatgcgcat
    11881 cagcgggaca gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg
    11941 ggaaatgttc gtttttatga tgaacagggg ttatgtgatc agctttatac ccacctcgcc
    12001 caggcgttaa atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc
    12061 gccagactgt atccacgcct ggtacgcacc acccgcgcgg cgctggccgg atttgaaagt
    12121 gaatacggcg tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc
    12181 tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat
    12241 agcgagcgag aagcgcagat tgagcaacag ttacgcgaac taacgttact gccgctcaac
    12301 attaaacata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg
    12361 attattgctc cttataccat gccgttaccg ctcttttcac caccgctgat ctatacggac
    12421 ctgacactga caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg
    12481 aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatca cccagaacac
    12541 gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc
    12601 gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc
    12661 agcaatatag cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg
    12721 caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc
    12781 gcaaatcgct gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact
    12841 cagtgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga
    12901 ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc
    12961 cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca
    13021 cgctgaccaa ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg
    13081 gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcacga ggatcacccg
    13141 ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga
    13201 gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag
    13261 tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat
    13321 cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc
    13381 cagcacgcgc aaggcggaaa tcaggcgaga aggatcgctg acgcggggcg cgatgagcaa
    13441 actcccgaga aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt
    13501 taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa
    13561 gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg
    13621 tcaggaggcg ttagtctgga gttaatctaa tgttggtgca agttgaaaaa taagaatatt
    13681 cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actcaaaaac tgcgatccgt
    13741 gacgcatgtt gcaaagataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac
    13801 aatggcgatg aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa
    13861 agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgcccgg tattaaaatt
    13921 attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac
    13981 cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgtg caaaccacgc
    14041 tttaacgatc ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc
    14101 atccagttgc gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac
    14161 caccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg
    14221 gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat
    14281 taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt
    14341 tggcagtaaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg
    14401 aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc
    14461 ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa
    14521 cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt
    14581 atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg
    14641 taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa
    14701 attaactatt tcacatatta gcaaccaacc agcaacatcc ttatgacaca aaaatagaag
    14761 gtcaatacat cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt
    14821 taccaactga ttgacaccca aaacggtcgg gtcactccgc gtatttatac cgacccggac
    14881 atttaccaac tggagcttga gcgtattttc ggtcgttgct ggctatttct cgcccacgaa
    14941 agccagatcc caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcagttgtc
    15001 gtggtgcgcc agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc
    15061 atgcgtgtga gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc
    15121 tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca
    15181 caagggttgt gtaaatccca ctgggggcta aacgaagttc cttgtgtgga gagttataaa
    15241 gggctgattt ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac
    15301 attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc
    15361 ggcgtacaaa agtgggtgat caactgcaac tggaaattcc cggcagagca gttcgccagt
    15421 gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat
    15481 gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc
    15541 aaagatgccc tgcaatttgg tcaggatggt cacggcagcg gcttcttctt tactgaaaaa
    15601 ccagatgcta atgtctgggt cgatggcgca gtttccagct attaccgcga aacctatgcc
    15661 gaagctgaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatatt
    15721 ttcccgacac tttcctggct caacggcact gccacactcc gcgtctggca tccgcgcggc
    15781 cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt
    15841 aaagccgctt ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag
    15901 gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac
    15961 agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct
    16021 ggcattacta actatatctt ttcagaaact gctgctcgtg gaatgtacca acgctgggcc
    16081 gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag
    16141 gaggtgatga aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc
    16201 tctttcacga agccagctta ctggacgact ggaaatttcg tgactggctg gcacagctcg
    16261 acgaagagat ttgttacacc atgcgcacca cggtgaacgc gcaaacgcgc gaccgccgca
    16321 aaggtgttca gccgccgaca acctggattt ttaatgacac caaagaccag ctggagcggc
    16381 gaatcgcccg tctggaaacg ggcatggcat gggcagaaga gccgccgtca cgcacccgcc
    16441 acttaatcag caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgttcggg
    16501 tgaattatct gctttatcgg gcacaaaaag agcgcgatga aacgttctat gtcgggactc
    16561 gttttgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg
    16621 tcctggatca agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat
    16681 ttatgcgtgt cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc
    16741 cgtcatcgcc ttgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca
    16801 tggtaatgcg tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct
    16861 acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc
    16921 gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc
    16981 gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta
    17041 gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg
    17101 tcggcggcga aagtcgccag tctgcgtcag cgatttggtg aacatattct ggcggtggaa
    17161 ggcaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc
    17221 ggcaagctgg attgttttat cggcaatgcg ggcatctggg atcacaatgc ctcactggtt
    17281 aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caacgtactc
    17341 ggttacctgc tgggtgcaaa agcctgcgct ccggcgttaa tcgccggtga aggcagcatg
    17401 attttcacac tatcaaatgc cgcctggtat cccggcggcg gtggcccgct gtacaccgcc
    17461 agtaaacatg ccgcaaccgg gcttattcgc caactggctt atgaactggc tccgaaagta
    17521 cgggtgaatg gcgtcggccc ttgtggtatg gccagcgacc tgcgcggccc acaggcgctc
    17581 gggcaaagtg aaacctcaat aatgcagtct ctgacgccgg agaaaattgc cgccatttta
    17641 ccgctgcaat ttttcccgca accggctgat tttacgggtc cgtatgtgat gttggcatcg
    17701 cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcaatt
    17761 cgcggcattc gccacgtagc ggctgggttg gatctttaag gaagtacgat gaaagaaaaa
    17821 acgatcatta ttgtcggggg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag
    17881 caagggttca ccggtgagct gcatctgttt tccgatgagc aacatcttcc ttatgaacgc
    17941 cctccgctct cgaaatccat gttgctggaa gattccccac agttgcagtc tgtgttaccc
    18001 gctcactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacattg
    18061 ggccgcgaca cacgagagtt agtgttagcc aacggcgaaa gctggcactg ggatcagctt
    18121 tttatagcaa ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc
    18181 tgctttaccc tgcgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa
    18241 cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg
    18301 cagcgcagat gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca
    18361 ccaccgcccg tgcaacgcta tcttttacag cgccatcagc aggctggtgt gcgcattctg
    18421 ctcaataatg ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt
    18481 ggcgagacgc ttcaggccga tgtggtgatt tacggtattg gtatcagcgc caacgaccaa
    18541 ctggctcgcg aggccaacct tgatactacc aatggcattg tcattgatga ggcttgccgc
    18601 acctgcgatc ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt
    18661 gcactacacc gctgcgaaag ctgggaaaac gccaataacc atgcgcaaat tgccgctgcc
    18721 gcaatgttag ggctaccgct tccgctactg ccgccgccgt ggttctggag tgatcagtac
    18781 agtgataact tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac
    18841 ccggaaaccc agaaagcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggta
    18901 acgctgaatc agggacgtga gattcgctca atccgcaaat ggatccagag cggcaaaacg
    18961 tttgatgcga aacagctgac agatgagaac atcgcgctta aatcactgta accaggataa
    19021 ttagcgaata tctcaacgcc tggggcgtgg cgaggtacca gagtgtgtat tacgtttaaa
    19081 tcacattatc ttgcaaaggg attggttatg aactcactac gttattttga ttttggtgct
    19141 gcccgccccg ttttgttatt aattgcccgt atcgccgtgg tcctaatttt cattattttt
    19201 ggttttccca aaatgatggg ctttgacggt acggtccaat atatggcctc gctgggcgca
    19261 ccgatgccga tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta
    19321 atcgtgcttg gctttttcac ccgtccgctg gcggtactgt ttattttcta cacgctgggt
    19381 acggcggtga ttggtcacca ttactgggat atgaccggcg atgccgtcgg gccaaatatg
    19441 attaatttct ggaagaatgt cagtatcgct ggcgcgttct tgctattggc aattaccggg
    19501 ccgggggcaa tttctctcga tcgccgttag gaaagatgcc ggatgcggcg tgaacgcctt
    19561 atccggcatt ttataaatta cagccactct acccgcaacg acatctctga ggttgttgat
    19621 tcccccggcg caagcgcaat gaggtcaccc ccttccgggc gatgatggtc atccggcgca
    19681 tggctcatcg gctccagaca gaagaaatca aacgcatatc ctttatcaaa cgccgggtcg
    19741 gaaacaaaga tgaaataaca cggtgcaggt ggcgtcgttt ctatgatgat ggcataaccc
    19801 tcctgcggct gctcgatgcg ggcctgacca ttccatccgg caaaaccatt gttcacccac
    19861 tggcgcggca acggcgctag ctggttaaaa tccagttctt gcggtagctg ctcgcaaaac
    19921 tcacccgcca gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc
    19981 gtttgcggcg acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc
    20041 ccttgattgg tgacagagag cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga
    20101 cttacccgat agtgatagac accgctgcga tgttcataca ccagacacaa actatcatcg
    20161 ctgtgcgaga cacattgcca ctcgcccagc cagccatcgc cgtgtagata gtgcgcatcc
    20221 cactcaacat tcggttgcag ttgatactca cgcccctgcc agacaaaacg gttgccgctc
    20281 acccggtttg caaatggcac cagcggaaaa caagaggcat cggttgccac accgcttttt
    20341 ttaccaggac gtaagagcgg cgtcgtatcg cgccagaagc cttcgattac gccgccctgg
    20401 tcagaaacgc ccagctttag cgacccgtgg gataaggtat agatggtcat ccgctaactc
    20461 cttaatccgg gaagttaatc acaactttcc cgcatttgcc gctcgccatc agcgcatagg
    20521 catcacctgc ctgttccagc gagaagcgat gggtaatggc gttacgcggc cacagcttcc
    20581 agtcagtcaa atcgtgggcg catttttcca tatggaacag actggtcacc caggagccaa
    20641 taatccgccg ttgatggtgc atcagatcgg cgctgacctc gaattccact tttccggttt
    20701 caccaatgta aaccacccgc ccccagtcag cggtggattg cagtgccagt aagcgacctg
    20761 cggcattacc ggaacaatca agcgcaacat ccgcgccgcc gtgagtgagt tcggcgatta
    20821 tctgcggcag accttcagtg gtcgctaaat agccgtgatc catcactcct aactgttttg
    20881 ccatcgccag acgttccggc agcatatcaa cgccgataat ccgttttgca ccgcgacctt
    20941 tcgccagcat catccccatc atgccgactg gccccagacc gaccaccagc acgttatcac
    21001 tgccggaaac ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactgataa
    21061 acgcaccatc ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca
    21121 gcaagtattc ggcatgaccg ccgtcacgct gccagccgta agccgctttt ccttcgccag
    21181 tacaagaaat aggaaaaccg cgacggcagt tcgggcaaaa accacagcca gaaatgtgat
    21241 acaccagcac gcggtcgccc tctttaaaat ggcggcagcc ttgccccatc gccacaatct
    21301 gcccgcacgg ttcatgaccg ttgataaatc cctagtacaa cggtttatcg gatgccgccg
    21361 ccgtagcgcg gtgctggtgg tagatatagt ggacatcgct tccgcaaatt ccggaggatt
    21421 tcattttgat cagtacctgg ttaatccccg gcgtcggcac tgcaacttcc cgcagatcga
    21481 cggtcgaatt tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa
    21541 tacgattaat tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc
    21601 tttactgcta cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc
    21661 gcgcaccacc tgctggaaaa aggagttaat cccgagcagc accagaccgt taccgattag
    21721 cgtaatcacc agtacgccaa gcagcgtacc gaacaacgag ccgcgaccac cggaaagcgc
    21781 cgtaccgccg accacgaccg cggcgatgac gtcaaactcc agaccgtttg cggcacctgc
    21841 gttaccggaa ccgaggcgcg ccgccaacaa aatgccggtc accgccgcta ataatcccga
    21901 aagggtaaag ataagaatgc gtacccgacg aacgttaata ccgcacaact gcgccgccgt
    21961 ggcattaccg ccaacggcaa aaaccgagcg cccgaaggcg gttttgcggc taatgaacac
    22021 aaacagcgca aacagcacca tcatgatcag cgcggatacc ggcacaccga gaaattgtcc
    22081 gcccagccag tccagcacct cgttttcgtc aatcggcact ggcagagcgt tggtcataaa
    22141 caagcccatt ccgcgcaggg cgctccacag ccccagcgtg gccacgaaac ttggcacgtt
    22201 aaacacgcca cgcagcaccc cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag
    22261 cagacaagca atcgccaacg gaacttcaaa ttgcagcaaa aatgccaggc ataccgagac
    22321 aaaagccacc atcggcccaa cgctgacatc aatttcaccg gagataataa tcagcgtcat
    22381 cgcccaggcg gcaatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa
    22441 tgagataaag ccaggcgcgt tcagggagaa gaccagataa agaatggcaa tcaccaccag
    22501 caaaccgatc tcattaatat ggtgactgac aaattgtttg agcgagacgc tcttgccctg
    22561 cggcaatggt aatgacgaag cagacatctt caggttcctc ggtaaaattc atcagtgcac
    22621 agacagaatg gcggacatca gctcatccac attgaccgga gcgtgaaact cctgcgagaa
    22681 cgtgccgtgc tgtaataaca ggatgcggtc acacaccaac ggcaactcct ccacttcact
    22741 ggagataaac accacgcttt ttccttcggc agccagctca cggacaatac ggtaaatctg
    22801 ctgtttggct tcgatatcga cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc
    22861 ggcatagacc caacgaccga tcactacttt ttgctgattg ccgccagaaa gcgtgccgat
    22921 gggcgtttcg ctactggcgg ccttgaccgt catccgctgc atcacttctt cggtcaggcg
    22981 gcggatggtg gaccattgca gcactccgtt ggcgctgatt ttttgccgat tggtcagcac
    23041 tgtattttcg tcaacgccca accagggaat gatccccgct tctttgcggt tttctggcgt
    23101 atagccaatg ccgcgtttca gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt
    23161 gataacaatt tcgccctgtt catacgtctc cagcccaaca atcgccttca gcaattcact
    23221 gcgccctgcc cccagcagac cagcaatgcc gagcacttcg ccacgacgta gcgtaaagct
    23281 gatatcctcc agcttgggct tatggcgtaa cgcacggact tccagcacgg cctgatccac
    23341 aatttcctga ggtgcaaccg gcgcaatatc aacgtgatcg cgccctagca ttagcgacac
    23401 aatatgatgc gtggaagtgt tttcgagcat cacatcgcca gccacctgcc cgtcgcgcat
    23461 aacggtggca ctggaggcaa tgcggcgaat ttcttccatc cggtggctga cataaatcac
    23521 cgccacgccc agtgctgaca tctttttcac cgctctgatc accagttcaa cttccgcact
    23581 cgcaagcgaa ctggtaggtt catcaagaat gaccacgcgc ggctcgccct tcatcacccg
    23641 cgcaatttcc accagctgct tatgcgccgg gcttagtgtt gaaacaagtt gttcaggact
    23701 aacgtcaacg cccagcgcct gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc
    23761 aatcatgccg ttgcggcggg gccactgacc gaggcagagg ttttccgcca ccgtcagccc
    23821 ttccaccaga cttaactcct gataaaccgc gcgtaccccc agttcagcgg cacggcgagt
    23881 cagcgtagct tcgtcacctt ccagtcgcgt ctcgccaatc cagatttcac cgctatccgg
    23941 acgttcactg ccggtaagca ttcgaatgag ggtcgatttg cccgcgccgt ttttgcctaa
    24001 cagcgcacga acctcgcctt tattgagcgt gaagttaacg ttatccaacg caacgacgcc
    24061 gggataacgc ttatttcctg ccaccacttt tgctaccggg actgcctctg ttgccgtgaa
    24121 catagggacc tctgcgaatc agcgattagg gcagaccatc aacgtgcgtt gccagccact
    24181 gtttaccgtc ttccgttttg gtatagagat cgataggcac ctgaatcact ttttcaccgt
    24241 cggcttgttt attgataacc ttcaatgttt gcgcgaaaac agcattgccc attttcttac
    24301 cggaaatatc cactaccgct ttcagcacct gattgttttc cagctcctga gcaatttcgg
    24361 ttgtcatatc cgaaccgaaa acagcaattt ttccggcctg attttgatta cgtaccgctt
    24421 ttaccgcacc gagtgtcgca ccgcccgatt cccccataat ggcgttgaga tccggcgtgg
    24481 agataatcag tttttcacca acggaaatcg ctttatctaa aacagtccct tcctgattag
    24541 cgacaatttg cgcgccggga acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct
    24601 gcacacaaac ttcaaaggct tcgcaattga tgacggcaat tttcggctgg tcaattttat
    24661 tggcaataaa ataatcggcg gcagcgttac ccagtttttt accaaattcc agcgggtcac
    24721 ccaccagata cgccgagaca tatttatcga cccccttttg attaatacag gtgttgtagc
    24781 aaatcaccgg aatacccgct tcactggcgc gacgaacggt acggctactg ccattttcag
    24841 acactgccga taaaataata gcatcgacat tacgcgccac gagggtatca acaaaggtac
    24901 tttctttcga aatatcaccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat
    24961 cttttgccgc atcctgaact ccctgacgca ctccagcgta atatccctga gtatcaaggt
    25021 atattgcgcc aatggtcatt tctttatccg cagctctggc aaatagtgcg ctacctaata
    25081 gcgtagccat taataataga ttacgggttg ttctcatttt tttaggcata gagcctcctg
    25141 tagggttttt attaacaacg gcttattcta attattttgt gatgagcggc agcgcgtgcc
    25201 gctcaaatat tacagttaat agggtttagt gaacgtagtt aaaaataaaa ggcatcacgg
    25261 tggtgaataa tgccgctttc ggccaggccg gattaatcgc cgtcaattca gcgcgagcag
    25321 attcgtattg cgcaatctcg cctaatcccg cggccgccag catcgccacc atcaggcatt
    25381 tttctttatg ctgctgttgt aaatcgccat acagcgacaa caagtcaggc tgcgagacgg
    25441 caaagaaatc cgcttcgata ctggttttcg ccatctcttg cgcccactgt ttcatttcac
    25501 taaacagttg ctgtgcggtg tgttgttcgc ccagtaatcg cagcgccatt ccttgccaga
    25561 agaggtaatc aaccggctga tcgttgtaat agctgtggat gttaatggtg cgatcgccgg
    25621 tcgccgccag atgtaaacag cgcgtcgctt cagtttcatc gccctgggct ttggcgcata
    25681 tcgcctgcca gaaccagatg tcgttatcag tttgccccgg taaacggcct tcgcttaaat
    25741 tctccggata atgcagcgcg gcatgaagca gttcgctggc ctgctgcggc tctctggcat
    25801 caagatgctg ccaggcgcgt aataactggt tgaggataaa ctgactggtg accttccctt
    25861 ccccgccttc ccacgggtgg aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct
    25921 gccccgtgag atgccacaaa ttgagcagtt ctgcggtcat gtcgtcgcgt ttcagcgcaa
    25981 tttccagatt attttccaga cgcgccagtc gtttctccgg tgtggttcca cttagcttat
    26041 caagcaaatc ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat
    26101 caagataacg cgcggccagc tcataatcgt gttgcttatt ccacgcatgg atcgctaacc
    26161 cgcgccagcc gtcggcaaac tccggcgaca tctctacgca acgttgccat aaggcaatgg
    26221 cttcgttgta gctacgtttg ttgtagtaga agcaggccag taaatggcga gcaaaccagc
    26281 actcttcaat actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact
    26341 gcgggaagac atcaatggct tttgcgacca gttcgccacg ttcggctttc ggcagcaagc
    26401 tggcttgcag gtaaagcggc agcgtgcgct ggcagtccag tgcattcagc atctctgccg
    26461 cgagggtggg cattccccag ttaatcagtt gcccggcggt catcagggcg ttaacgtcgc
    26521 gtccctgaca cagaccgcgc cactgcgcga gagctgattc gctacgacca tcgaaccagt
    26581 tcaggcacca cagagtggcg ttcagcggat aatcgcgcag cagtttctcg cgctgcaaac
    26641 gcgcgttgtc ctgacgacca cttaacacca gcagcagatt atgcaggcaa agcacttcct
    26701 gattgattgg gcaggcgcga agactttgtt ggcaaaaatc cagacccgcg tcgaagttac
    26761 cattacgcgc cgccagtcgt gccagaccat aatagcctcc ggctttactg ttgccgctcc
    26821 agaccgcacg ccagaaatcc tcttcggctt gttgatattg tccctgacgt tcgtaagcac
    26881 tggcgcgaat caaactcgcc tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt
    26941 tcagagcctg agtggcatac gccaccgctt gcgggaaatc tgcgcggtta tactccagca
    27001 tcgccagcgc cagattacag cggtaatcca gcggatccag cgccacgccg cgcaggtagt
    27061 aatcgaacgg tgaacggctg gcgtgatgat attgctccag atgctgaccg ataaaccagg
    27121 cttcatctgt actggtaatg tcttgtgctg acagtggcgc tttggcgacg tccggcaacg
    27181 gcaactcttg cggctgatgt tcctgataac tcagtacaat attgccgtcg gcatcagaga
    27241 gctcaatggt cagtctttca ggattgatac cgtgtaacac gccctggatg gcggtcgctg
    27301 gtgtcagggc cacggcatca tcgagcaacg cgtcgcattt gccgatttcg cggatcgcca
    27361 ggcggtatcc gttcaacgga gagatggcat acagccccca ctcaatcccc cgctcactac
    27421 gctggagttt gattaccgca tcgcgggagg cgttttgcac catgcccaga gaatggtaag
    27481 gcaggaaaaa ctgctcaaaa cgcttctctt cgtaagcatc aagccgggta aaatcaggct
    27541 ggttatcggc aaaaataccg gtcatcagtt cgatatacgg gccgttatta tcagttaggc
    27601 tcttatccca cgcctggcca aattcactgt gtccccagct ccactgcttt ttacctggcg
    27661 caatatggtg gttggcaacg tgtagcaaac cgccatcttc atcgtgacac cacgcgccaa
    27721 caaaatcgta ctgtgatttt tcagccatat atgaggttgg aacgggcaca tttttatagc
    27781 gagaaatgtc cactccagcg gagtagtcca ctttgtagta agtgccggtg gcgataggga
    27841 aagcggagac ggcccgtttg ccgtgatcaa acaccgccgt tacatccggc ggaaagacgc
    27901 tctgatgccc ttcaccccct ttcactgccg ggttggccca ccacaagaaa tgacgcggcg
    27961 tggcgttgcc gttatagacg cggctggcga tttccagcgc cgcccggtca gggcgcaggg
    28021 tgaaacctgt catcacctgt aaaccatgca tcggctccgt ttcgcctacc cacaccgtct
    28081 gtgcgccgtc ttcatgggct tcgagggtga aatcaacggg cataaaggtg gtcgggcgat
    28141 ggtgttgcgg ccagttaaac tcaatcccgc cggagatcca cggtcccagc agccccacca
    28201 gcgcaggttt aatgacttca ttgtgataga caaaatcgcg ctgtttcact ttatcccatg
    28261 cgcgatgcac ccgaccgccc aattccggca ggatcatcac tttgatgtag tcgttttcca
    28321 gccacaccgc ctgccaggat ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt
    28381 agggataaac cgcgccggac gatccctgat aaacgcgatt ttccaggaac atgggatgta
    28441 tatcctgcgg cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg
    28501 gagtcatggt gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta
    28561 ttgaacaatc tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt
    28621 gaagatgaga gcctgcatta acaatcaaca gattcgccac cataacaaat gcgtgattct
    28681 ggaactgctg taccggcaaa agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat
    28741 ttcgattccg gcagtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa
    28801 tatcgacgat gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga
    28861 aggtgactgg acgctgtgtc tgaacgtgac gcccaccagt attgagtgtc aggttgctaa
    28921 tgcttgttta agtccgaaag gtgaatttga gtatttacag attgatgcac cgacgccgca
    28981 ggcgctgctg tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg
    29041 caccatcaat ctggcgctgg caatccacgg tcaggttgat ccagtgactg gagtgtcgca
    29101 aaccatgccg caagcgccgt ggacaacgcc ggttgaggtg aagtatctgc tggaagagaa
    29161 gctgggcatt cgggtgatgg tcgataatga ctgcgtgatg ctggcgctgg cggaaaaatg
    29221 gcaaaataat tcgcaggaac gggatttctg cgtgatcaac gttgattacg gcattggctc
    29281 gtcgttcgtg attaacgagc aaatttatcg cggcagtttg tatggtagcg gacagattgg
    29341 tcacaccatc gttaatccgg atggcgtcgt ctgcgactgt ggacgttacg gctgcctgga
    29401 aaccgtcgcc tcgttaagtg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc
    29461 ggttaatact cagcttgatc ctgaaaaact gactacagcg cagttaatcg ctgcctggca
    29521 aagtggagaa ccgtggatca ccagctgggt tgatcgctct gccaatgcca ttggtttgag
    29581 tctgtataac ttcctcaaca tcctcaatat taatcagatt tggttgtacg gtcgcagttg
    29641 tgcctttggt gagcactggc ttaatactat tattcgccag acaggattta acccgttcga
    29701 ccgcgacgaa ggaccgagcg tgaaagcgac gcaaattggc ttcgggcaat taagccgcgc
    29761 acaacaggtg ctgggaattg gctatttgta tgttgagacg cagttacgac agatttgatg
    29821 gcgcgataac gtagaaaggc ttcccgaagg aagccttttt caacagatta accgtttcgc
    29881 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    29941 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    30001 ttctacttct ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt
    30061 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    30121 gcccagagcg gcgtctgctt ctttaccggt caggttttta tcgaccagat caaccaggaa
    30181 cagatggtta tcagtaccgc cggaaaccac tttgtagccg cgttccagga acacttctac
    30241 catcgcttta gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    30301 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    30361 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat
    30421 cagaccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    30481 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    30541 gaacaggtaa gcgccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc
    30601 ggaatatgca gagaagccac cgataatcat tttcggtttg tgttctttgg cttgtttttc
    30661 cagatcggcg tagtcgatat gaccggtagc atcaataccg taaggaacaa tgttgtacag
    30721 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    30781 acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    30841 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    30901 cgccagttgt tcaacgatat caacatactc gcaaccaccg tagtagcgtt tgcccggata
    30961 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    31021 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    31081 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    31141 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    31201 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    31261 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    31321 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    31381 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    31441 atgcaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc
    31501 atccctttac tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt
    31561 actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa
    31621 cgtgaagccc tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg
    31681 ctgctgccag cggtagaaaa aatcgcgcag aagcacacca gtttccagat caaaccggaa
    31741 cagtacaaca tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg
    31801 caggaagtgc tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat
    31861 cgcgaggcgg aaatctataa cgaaaacgcc agcaaagccg gtggctggga aggtactcgc
    31921 gatttccgca ttgtggctaa aacacctcgc agcgcgctta tcaccagctt cgaactggag
    31981 ccggtcgacg gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg
    32041 aagccggaag gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat
    32101 ggcaaaggct atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg
    32161 cacaatcacg ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt
    32221 atggctgtcg cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca
    32281 atgctggcaa tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc
    32341 catgcggcag aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag
    32401 tcactgccgc gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct
    32461 aaaggtcagt ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc
    32521 gatccgacaa tgcagttcta tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa
    32581 cagttagtgg atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat
    32641 aaggtgctgt gatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa
    32701 attgccgcgt cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg
    32761 aagattttac cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag
    32821 gtatcgacaa tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc
    32881 atatactccg cgccgcggta cagctcggta tggcctttct ggcggccaaa acctttcacc
    32941 tcagtgaccg tcatgccggt aatgccgact tcagccagtg cttcgcggac atcgtccagc
    33001 ttgaagggtt taataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt
    33061 cttttgatct gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc
    33121 agctcgtgtc gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc
    33181 gcgtgggtga cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac
    33241 tgattacgtg cctcaacaaa ggttggcagc gccgtatttt caccctccag cgcctgctct
    33301 actaatgcat cgctaatcac cggagatgag gtcagcgcca cgcactgttc aatcacgttg
    33361 accaactggc gcacattacc cggccagctc gcggtcatca ggcgtttcat cgcatcggtg
    33421 gaaaacgcgc ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc
    33481 agtagcggaa tgtcttctgt gcgctccgcc agtgccggaa ttttcaggct gacaacgttg
    33541 aggcggtaat agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg
    33601 tgagtggcag aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc
    33661 actttacgct cctgcaacac gcgcaacagt ttgacctgca acggcgcagg catatcgcca
    33721 atctcatcga gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg
    33781 acagcgccag taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctca
    33841 ggcaatgcac cacagttaat agcaataaat ggtttgctat tgcgcgggct ggcgttgtgg
    33901 atagcctggg cgaaaatctc tttcccggtg ccgctctggc cattaatcaa aacgctgacg
    33961 tctgattgcg ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg
    34021 acaatcgcct cgcgccagcg ttcgtcggtg gctggcgcgg attgttccag cgcatcatca
    34081 attgcctgat atagcgcgtc tttatcaaca ggcttggtga ggaaactaaa aacgccctgc
    34141 tgtgttgcag caacggcatc gggaatagaa ccatgcgcgg taagaataat taccggcatt
    34201 cccggctgca ctttctggat ttcagcaaac agctgcatac cgtccatttc atccatccgc
    34261 aggtcgctga tgactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca
    34321 ctttccgccg tgaccacgct gtagccttcg ctggtcaggc gcaggccaag cagtttcagc
    34381 aatcccggat cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac
    34441 ctcatgggtg gatggcgcgg gcttttcact ctcgtgtggc gtatccggcg agaaatttcc
    34501 agccggtttg cgggtcgaga gctggcgttc aatatcagtc aggttttcca gcttgcgggt
    34561 ggtgagttcc agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct
    34621 gtcgctggac tgctggagtt tactgtagcg ttggcgctct tccgccagtt gcagttgcag
    34681 cgcctgaccg tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact
    34741 taacgcttca atacgcgcga ccagctggcg gcgctcatat ggcgtaattt tggcgtcggc
    34801 gagcaaaatc ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgagcttg
    34861 ctgacgcgac tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag
    34921 cggattggtt tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag
    34981 atagtcagcc agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt
    35041 attgtgattc tgcacgcaac ctagcaatgc cagacatggc agccctgcca gccacagccg
    35101 tcggggcaat aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag
    35161 tgggcgtaat tgttgcagac agtttggaca tggacagcgc ggagaaaccg gagcgtacat
    35221 gtcgtacgtg aggatttcga gcactgcccg gggccgaact gacaaataaa atagcctgat
    35281 gggataggct gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa
    35341 acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc
    35401 ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc
    35461 tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg
    35521 atgacatcaa tataaacccg cgcaccatgt aaactgctgc gaaggcaaat gttaccggat
    35581 tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt
    35641 ggctccgcca ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt
    35701 gcgggcaggc tatgagcaga aactactgtc tccaccagcg gtgctaactc aacattatct
    35761 agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt
    35821 tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc
    35881 ggcccgacaa cctgatcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt
    35941 tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg
    36001 cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac
    36061 gaaacgctat tgcccagaga acgcccttcc cccaaccgat tgatcatccg ttcaatattt
    36121 ttcaccggcc cgataatcat ccgcgtgaaa agcagtacca ttaccagact caccagaaat
    36181 agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc
    36241 tgcccacgag agaacactac tgtgcgcgtg gcctgtacca tttcggtatt ggcactggca
    36301 aaggcttcca gacgcgcggc agcagcagca tcgggaccac tgttgttgca ctgaagttgg
    36361 gccagattgt tcaagtcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct
    36421 gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc
    36481 gtagggtcat ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg
    36541 gtcattgctt cgctgcgccg ggcatcgata agcgtggtgc ggttgaccag cgccgcctgg
    36601 tcgctaagcg cattcaggct ttgccaggct tgccatgcca gcaccaacag gggcagcaga
    36661 atcagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt
    36721 ttcaaggtgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga
    36781 tacaacaaag ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg
    36841 tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt
    36901 tgtgcgtcat tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg
    36961 agccactgat tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac
    37021 tgattatacg ttgctttgag gggtttatgg cttccccgct atttgaatgt aaatcagcca
    37081 ctgtgtcgct aaaaagagac aacttaagaa aaacttatta gattacaata aaatcaatca
    37141 tttaagtgtc gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa
    37201 aaaagctccc gaagttggga gcttataata gcggttgtat ttaatgccgg atgcggcgtg
    37261 aatgcctcat ccgggctgca aaaccaatga gtttgacggc ttaccccaac tgcttacgtg
    37321 cattgcggaa aatgcgcatc catgggccat cctcgcccca gttttcagga tgccaggagt
    37381 tgctgacagt acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccgcttt
    37441 cagtcgtgac cgccgtaata ccgttcggag aaccgttcgg gttagccggg taggtttcag
    37501 tgactttgcc gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg
    37561 ccagatgcgc cgcatcacgc acttccacgc gcccttcacc atgagagaca gcaatcggca
    37621 tttgcgagcc caccatcccc tgcaacagca gcgacgggct ttgggtcact tcaaccaagc
    37681 tgaaacgcgc ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac
    37741 ctgggatcag ttcacgcaga ttagacatca tctggcaacc gttacatacc cccagcgcca
    37801 gcgtttgcgg acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga
    37861 tagacttcgc ccaaccttca ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg
    37921 cgaccagggc gtggaaatcc tccaggcccg tgcgtccagc cagcaggtcg ctcatatgca
    37981 cgtcgatagc atcgaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca
    38041 cgccctgctc acgcagcaca gcaactttcg gacgtgcgcc agtggcaata tacggtgctg
    38101 ccacatcttc gttgatatca aacgacagtt tcacattcag gcccggatcg gcgtcgtttg
    38161 atttcgcctg atgctcctga tcggcacact ccgggttgtc acgcaggcgc tgcatctgcc
    38221 aggtagtttc tgcccaccag acacgcaacg tagtgcggct ttcgctgaat acagtctgcc
    38281 cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa tggacgcaat
    38341 cagcaagccc atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct
    38401 gaatgaccgc acccagttct tcgttaaaca acgccgccag gcgatcgtca cccagagtgg
    38461 cgatatccgc gtcaatgccg caatgaccag caaaggccat ttccgccagc gttaccagca
    38521 gaccgccatc agagcggtcg tgatacgcca gcaacttacg ctgtgcaacc agcgcctgaa
    38581 tcgcgtcata gaagcctttc agttgcgcga catcgcgtac atctgccggt ttgtcgccaa
    38641 gctgacgata aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat
    38701 caatcagcag cagtgcgtta tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat
    38761 cttccacgcg ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt
    38821 cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtca
    38881 ggcccagcgc cggacaaagc tcttcgccca cggctttaac ggcttcatac aggcccgcat
    38941 cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat
    39001 cgccaatctg tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg
    39061 cggcgaaatc cagcagcgca accggcgcac gctcgccaat cgccatcgct tcaccgtagt
    39121 agctgtcgag gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggcccca
    39181 ccatctgatc gcgcgccacc atgccggtta cgctgcggtc accgatggtc accaggaaag
    39241 ttttctccgc cacggtcggc agatgcagta cgcgtttcac cgcgtctgca atggtgatcc
    39301 cttcacggac cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg
    39361 gcgttttacc aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat
    39421 cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct
    39481 cacgcttaca cagttcgtca aacagcggta attgatcggc agcaaccgcc agcacgtagc
    39541 gttcctggga ttcgttacac cagatttcca gcgggctcat gcccggttcg tcgcttagaa
    39601 tatcgcgcag ttcaaattta ccgccgcgcc cgccgtcgct caccagttcc ggcatcgcgt
    39661 tagaaagacc gccagcgccg acgtcgtgga taaacaggat tgggttggca tcaccaagct
    39721 gccagcaacg gtcgatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta
    39781 cggaagcaaa gtcgaggtcg gcatcagact gacctgacgc catggaagac gccgctccgc
    39841 cgccaagacc gatgttcatt gccgggccgc cgagaacgac cagtttcgca ccgacgttga
    39901 tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg
    39961 gtttgtgata accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac
    40021 ggaagtagcc attcagtgcc ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg
    40081 ggccttcggt catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt
    40141 cccacggctg ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg
    40201 ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccggaaccgg
    40261 tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca
    40321 gaatatgcgc cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc
    40381 ggcccacttc agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtgat
    40441 ctggcgtggt ttcgaaagta tttttgatca tcttgaacag cgatttcggc tgctgttcac
    40501 cgtcgataat ccagtcggca ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag
    40561 cgaacatata cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca
    40621 gatagtcaat ttcatcttcc gccagagcca ggccaagacg caggttagcg tcgatcagcg
    40681 cctgacggcc ctgccccagc aaatcaacgc tggtaaccgg agtcggttga tggtgggcaa
    40741 acaactgctc tgcatcatct aaagcaaaaa agacagtttc catcatgcgg tcgtgcagtt
    40801 cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa
    40861 cgccgcgctc aaggcggttt acctgttgta gcccgcagtt atgagcaata tcggtcgctt
    40921 tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg
    40981 gggcgtggct ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat
    41041 catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa
    41101 ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg
    41161 caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc
    41221 caagggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac cgtttgcgtg
    41281 gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta ataagttgcc
    41341 atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc actattatta
    41401 catt
//
LOCUS       DBFIFS010000018        40281 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.018, whole genome shotgun sequence.
ACCESSION   DBFIFS010000018 DBFIFS010000000
VERSION     DBFIFS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.018"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(214..2073)
                     /gene="gss"
                     /locus_tag="N7A07_000893"
     CDS             complement(214..2073)
                     /gene="gss"
                     /locus_tag="N7A07_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU2475613.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2278..3144
                     /gene="yghU"
                     /locus_tag="N7A07_000894"
     CDS             2278..3144
                     /gene="yghU"
                     /locus_tag="N7A07_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU2475614.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3267..3515)
                     /gene="hybG"
                     /locus_tag="N7A07_000895"
     CDS             complement(3267..3515)
                     /gene="hybG"
                     /locus_tag="N7A07_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU2475615.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3528..3869)
                     /gene="hypA"
                     /locus_tag="N7A07_000896"
     CDS             complement(3528..3869)
                     /gene="hypA"
                     /locus_tag="N7A07_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU2475616.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3862..4350)
                     /gene="hybE"
                     /locus_tag="N7A07_000897"
     CDS             complement(3862..4350)
                     /gene="hybE"
                     /locus_tag="N7A07_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU2475617.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4343..4837)
                     /gene="hybD"
                     /locus_tag="N7A07_000898"
     CDS             complement(4343..4837)
                     /gene="hybD"
                     /locus_tag="N7A07_000898"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU2475618.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4837..6540)
                     /gene="hybC"
                     /locus_tag="N7A07_000899"
     CDS             complement(4837..6540)
                     /gene="hybC"
                     /locus_tag="N7A07_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU2475619.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6537..7715)
                     /gene="hybB"
                     /locus_tag="N7A07_000900"
     CDS             complement(6537..7715)
                     /gene="hybB"
                     /locus_tag="N7A07_000900"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU2475620.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7705..8691)
                     /gene="hybA"
                     /locus_tag="N7A07_000901"
     CDS             complement(7705..8691)
                     /gene="hybA"
                     /locus_tag="N7A07_000901"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU2475621.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8694..9812)
                     /gene="hybO"
                     /locus_tag="N7A07_000902"
     CDS             complement(8694..9812)
                     /gene="hybO"
                     /locus_tag="N7A07_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU2475622.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10001..10288)
                     /gene="yghW"
                     /locus_tag="N7A07_000903"
     CDS             complement(10001..10288)
                     /gene="yghW"
                     /locus_tag="N7A07_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU2475623.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10407..11294)
                     /gene="yghX"
                     /locus_tag="N7A07_000904"
     CDS             complement(10407..11294)
                     /gene="yghX"
                     /locus_tag="N7A07_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU2475624.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRILKWFDKYLS"
     gene            11451..12491
                     /gene="gpr"
                     /locus_tag="N7A07_000905"
     CDS             11451..12491
                     /gene="gpr"
                     /locus_tag="N7A07_000905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU2475625.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12531..13025)
                     /gene="yqhA"
                     /locus_tag="N7A07_000906"
     CDS             complement(12531..13025)
                     /gene="yqhA"
                     /locus_tag="N7A07_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU2475626.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13216..14100
                     /gene="yghA"
                     /locus_tag="N7A07_000907"
     CDS             13216..14100
                     /gene="yghA"
                     /locus_tag="N7A07_000907"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU2475627.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14372..14797)
                     /gene="exbD"
                     /locus_tag="N7A07_000908"
     CDS             complement(14372..14797)
                     /gene="exbD"
                     /locus_tag="N7A07_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU2475628.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14804..15538)
                     /gene="exbB"
                     /locus_tag="N7A07_000909"
     CDS             complement(14804..15538)
                     /gene="exbB"
                     /locus_tag="N7A07_000909"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU2475629.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15790..16977
                     /gene="metC"
                     /locus_tag="N7A07_000910"
     CDS             15790..16977
                     /gene="metC"
                     /locus_tag="N7A07_000910"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU2475630.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17117..17776
                     /gene="yghB"
                     /locus_tag="N7A07_000911"
     CDS             17117..17776
                     /gene="yghB"
                     /locus_tag="N7A07_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU2475631.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17816..18715)
                     /gene="yqhC"
                     /locus_tag="N7A07_000912"
     CDS             complement(17816..18715)
                     /gene="yqhC"
                     /locus_tag="N7A07_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU2475632.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18909..20072
                     /gene="yqhD"
                     /locus_tag="N7A07_000913"
     CDS             18909..20072
                     /gene="yqhD"
                     /locus_tag="N7A07_000913"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU2475633.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20177..21004
                     /gene="dkgA"
                     /locus_tag="N7A07_000914"
     CDS             20177..21004
                     /gene="dkgA"
                     /locus_tag="N7A07_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU2475634.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21204..22126
                     /gene="yqhG"
                     /locus_tag="N7A07_000915"
                     /pseudo
     CDS             21204..22126
                     /gene="yqhG"
                     /locus_tag="N7A07_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            22175..22432
                     /gene="yqhH"
                     /locus_tag="N7A07_000916"
     CDS             22175..22432
                     /gene="yqhH"
                     /locus_tag="N7A07_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU2475635.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22475..24694)
                     /gene="ygiQ"
                     /locus_tag="N7A07_000917"
     CDS             complement(22475..24694)
                     /gene="ygiQ"
                     /locus_tag="N7A07_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU2475636.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24805..26217)
                     /gene="ftsP"
                     /locus_tag="N7A07_000918"
     CDS             complement(24805..26217)
                     /gene="ftsP"
                     /locus_tag="N7A07_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU2475637.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26292..27029)
                     /gene="plsC"
                     /locus_tag="N7A07_000919"
                     /gene_synonym="parF"
     CDS             complement(26292..27029)
                     /gene="plsC"
                     /locus_tag="N7A07_000919"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU2475638.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27263..29521)
                     /gene="parC"
                     /locus_tag="N7A07_000920"
     CDS             complement(27263..29521)
                     /gene="parC"
                     /locus_tag="N7A07_000920"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU2475639.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29659..31266)
                     /gene="ygiS"
                     /locus_tag="N7A07_000921"
     CDS             complement(29659..31266)
                     /gene="ygiS"
                     /locus_tag="N7A07_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2475640.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31375..31857)
                     /gene="ygiV"
                     /locus_tag="N7A07_000922"
     CDS             complement(31375..31857)
                     /gene="ygiV"
                     /locus_tag="N7A07_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU2475641.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31910..32302)
                     /gene="ygiW"
                     /locus_tag="N7A07_000923"
     CDS             complement(31910..32302)
                     /gene="ygiW"
                     /locus_tag="N7A07_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU2475642.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32454..33113
                     /gene="qseB"
                     /locus_tag="N7A07_000924"
     CDS             32454..33113
                     /gene="qseB"
                     /locus_tag="N7A07_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU2475643.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33110..34459
                     /gene="qseC"
                     /locus_tag="N7A07_000925"
     CDS             33110..34459
                     /gene="qseC"
                     /locus_tag="N7A07_000925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU2475644.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34569..35150
                     /gene="mdaB"
                     /locus_tag="N7A07_000926"
     CDS             34569..35150
                     /gene="mdaB"
                     /locus_tag="N7A07_000926"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU2475645.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35181..35495
                     /gene="ygiN"
                     /locus_tag="N7A07_000927"
     CDS             35181..35495
                     /gene="ygiN"
                     /locus_tag="N7A07_000927"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU2475646.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35540..36427)
                     /locus_tag="N7A07_000928"
     CDS             complement(35540..36427)
                     /locus_tag="N7A07_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU2475647.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36424..37371)
                     /locus_tag="N7A07_000929"
     CDS             complement(36424..37371)
                     /locus_tag="N7A07_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2475648.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37382..38419)
                     /locus_tag="N7A07_000930"
     CDS             complement(37382..38419)
                     /locus_tag="N7A07_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU2475649.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38428..39411)
                     /locus_tag="N7A07_000931"
     CDS             complement(38428..39411)
                     /locus_tag="N7A07_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU2475650.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39408..40217)
                     /locus_tag="N7A07_000932"
     CDS             complement(39408..40217)
                     /locus_tag="N7A07_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2475651.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT        10056 a        10807 c        10084 g         9334 t
ORIGIN      
        1 attttttaac gataaaaaga gtgttatttc ttgtgtatca gacaataagc attcattaaa
       61 aatctttaga aataattaga acacaggcat tctgctggtt aatgtattca catatgaaat
      121 gaataaatat ttgaacatat aaatattgaa tattttgatt aatccccgta ctgactattc
      181 ttcataatca gtacgggatt gctacaacat gtattactct ttcaccacaa ttaacggttc
      241 aatatcactc tcttttttaa taaccagtga ttcatcaccg cgcaaacacg tcccaccgta
      301 gttgccgcca acggtgaagg tacatacctg aatgtattta ccgtccactt tcggcaaaca
      361 ccacagttgc tgatagatgt ttttctgctc ggcaaattta ccactggttt tgtccagcac
      421 ctcttcatga tggctgacga ggtcgatgtt gctgccacag cgaccggcga tcggtttcac
      481 tgcgtaacct gttttcacca attcatcatt aacagtgaaa tcggtatcca gcaggtaacg
      541 atggtgcggg aacagcgacc agaggatcgg cagaatcgct ttgttgccgg ggatcaccgt
      601 ccacagcggc tcaaagacca gcacttccgg gcgcagcaat acgtcgataa gacgcacttc
      661 attttgcgga tgaccggtac ggatcggcac cgcagcaaac tcacggtcgc taacttcacg
      721 aatctgatca aacgcggttt cccacgccca ggttttccac acgcagttaa ccagtcgccc
      781 ttccccatca atcagttgcc cggcagcatc ccagcccagt tcatccagcc cgcgcaagat
      841 acgcgtttca aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt gatagttttc
      901 ctcgatatct ttgtcctgca tgatatggac aaacggacgt gcacgactgt gtttccaggc
      961 accagccagt tcgttaatca gcccttccgc cggattgaag ccgttgcctt tatagccctg
     1021 ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga caggaggcgg agtcggcgtt
     1081 gtactcgtaa accttcagac cacgctcatc catgcagaaa tccatacgac cggtgatcat
     1141 atggtgacgg cgacgctgcc aggagagacg caaacgtggc cagaggattt tcggaatgtc
     1201 gaacagcgcc agcaggttgt catctttcag caccttgtcg gttgcgtgaa gatacatcag
     1261 gtgcagctcg ttggtggctt taatcagctc ctgctcggcg ctctcggtaa tggtgtagta
     1321 atgatacgga tcctgattga tcacctgacc gttggcctgc acataggcgt tttgcagcgg
     1381 atctttttca tccagccatt taccgtcaaa ctggcctttg ttttccagac gcgctccgct
     1441 gattttcagc agctcgcctg caatttccgg ctgcggtaag ctgtattcag tatcttccgt
     1501 ctggatcatc cagcccagaa tggtggtgtc atcaaaagtg tctttaaggg tatagcagcc
     1561 gttttcgacc accatctcca gttcgcgcgt ccactgttgc ccttgcggca acggggaatg
     1621 aatcacgttc tgttccgcaa tacggacttt gttgccatgt aattgggtaa tgatggcgac
     1681 atggccagtg tctttaaatt caccgccttt atcccagata agaagcgcac ccgcgaccgg
     1741 cgcacgcggc gagccgttag gaaatgcctg caatggcagg atgttgtcat taaccacttc
     1801 acgcaggaag cgcagcgaga aaatctccca cgccataccc acgtcagtaa agaccacacc
     1861 gtagttcaga aagagaaaac ggcgagcaaa ttcaacgcat tgccatttgt ggcccatata
     1921 ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg tattcctgcg gatcgagaga
     1981 actgtaatct gaagagtaga ttgctacccc acctggggcg tagcccaata atgtcccgaa
     2041 cggggcatcc tggctggtcg ttcctttgct catcacttta cctttaacaa tacaacctaa
     2101 gcagttggcg taattgttgt gctctgatta cctgctcagc gacattaacc ggacagaggt
     2161 cagactaatc atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa ttcacattct
     2221 gtacagcaag tgacctgcta cactgcttca acactaccac tcagaaggca actcactatg
     2281 acagacaata cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc tggcggcgcg
     2341 ttcgccaata tcaatcgccc ggtttctggt ccgacgcatg aaaaaacgct gccggtgggc
     2401 aaacatccat tacaacttta ttcgctggga acgccgaacg gtcagaaagt aacgattatg
     2461 cttgaggagc tgctcgcgct gggcgttact ggcgcagagt acgatgcctg gctgatacgc
     2521 ataggcgatg gtgatcaatt ctccagcggc tttgtcgaag tgaacccaaa ttcgaagatc
     2581 ccggcgctgc gcgatcatac ccataatccg ccgatccgcg tgtttgagtc aggatcgatt
     2641 ctgctttatc tggcggagaa atttggctac ttcctgccgc aggatctggc aaaacggact
     2701 gaaacgatga actggctgtt ctggttacag ggcgcggcac cgttcctcgg cggtggtttt
     2761 ggtcactttt accattacgc gccggtgaaa attgagtacg ccatcaatcg ctttaccatg
     2821 gaagccaagc gcctgctcga cgtgctggat aagcaactgg cgcagcataa gtttgttgcg
     2881 ggcgatgagt acaccattgc ggatatggcg atttggccgt ggtttggcaa cgtggtgctc
     2941 ggcggcgtgt atgatgccgc agagtttctg gatgcgggaa gttataaaca tgtgcaacgc
     3001 tgggcgaaag aagtgggcga acgtccggca gtgaagcgtg ggcgtattgt taaccgcacc
     3061 aacgggccgc tgaacgagca gttgcatgag cgccatgacg ccagtgattt tgagacgaat
     3121 acggaagata agcgtcaggg gtaaggattg gtgttcgtcg cagcaagcca tccaggccgg
     3181 ataaggcgtt cacgccgcat ccggcaatcg agcacaatgc ctgatgcgac gctgccgcgt
     3241 cttatcagac ctacgaaagc taatcatcac gcgctggtaa tgtcgtaatc catttggcgc
     3301 agtgcgtcta atgtggcttt ggcttcatct tcgtcgatga tgctcatggc aaatccgacg
     3361 tgtaccagca cccactggcc cagcagatcg gcagggttac cttcacaaat cagggcgata
     3421 ttcacgtcgc gcttgatacc acatacttca acctgcgcaa gctggtgaat atcttcaccg
     3481 acagccagca cctggcctgg aacgccaata cacattgtta actccggtta ttcaacttca
     3541 atacttttga cgatcagcga atcgccggta tcgacacgca accgctcgcc gtgacagagc
     3601 ggacactgcg catcgtgctg atgaacctcc accacctggc tgcaatccca gcaccaggcc
     3661 tgggcgggtt tatagacgat atgtaaatcg cacccttgcg ccaccgttcc gtggcagaca
     3721 atttcaaaac taaaacggac ggcgctctcc tcaacgcagg agagcgcgcc aatttccagc
     3781 cacacggcgg tgacgcgctt aacatcgtgc tgctccgcct gccgctggat aatttcaacg
     3841 gcgctctggc aaagcgacaa ctcatgcatt ttcgccactc ctgcgaccaa acagcagggc
     3901 gcgacgccct gcgtgtggta catccggatt cgtgactggc agcgaaagga tcattcgtgc
     3961 gcagtcatcc gtcaggcgtt ggccctcttc aatcgacata ctgtgcgaaa gcggtgacat
     4021 cagcgaacag gagaggtatt gcgaaacacc gtccagttcg ccaacggtaa aggtcatggt
     4081 gccatacggc agttgcagac caattttttc actcactttg cgcagcggcc agagttgatc
     4141 cgggccgggg aagataactg cactcagcat ccacggggtg atcacacacc ccgtccactg
     4201 accttcgaac agcgtaaaat cagaaacata caccggcatt gaaggatgca gaaaagagag
     4261 agcgtgcatc gaacgccggg caatttcttc aaacgctgcc tgtacttgcg ccttcgggga
     4321 ggtctggaaa cctgctatct cttcagtcat gaatcgcctc ccgtgggatg gcttccacgc
     4381 cagattcacg cagcgcagcc agaacctgct caagcgcagg ttcaatcatt gcttcaaccg
     4441 tcggcgttaa gccgatgtgt ggctccagcg attccggaat tacgccgacc agggtcagct
     4501 ttttcggaaa ctcgccggtg aagcgcaggg ccgacaagac gtcggccagg ccaagctgat
     4561 gcggagagat tttgttggta aacaacgccg gaacttcttc atcccgcagg atcatcatcg
     4621 ttcccggcgc gctcttcttc gacacaatgg catccgcaat aatcagatga tcgcgatttg
     4681 ccatgtcgcc aagcagctcc attcccgccg tgccgccatc gaggatctca acataatccg
     4741 gcagaatgta tcgttgctct aacgcttcga caatccgcac accgatggct tcatcggtca
     4801 gcaaaatatt gccgacccct aagactaaaa tacgcattac agaaccttca ctgaaaccac
     4861 ttcgttgccg tcagcatcca ctacgtgtac cgcacaggcc atgcacgggt cgaaggagtg
     4921 aatggtacgc accacttcca gcggtttatt cggatcagca accggtgtac ccaccagcga
     4981 ctgctcgtaa ggaccgacgt catcattgaa gttacgcggg ccagagttcc aggttgatgg
     5041 aacaactgcc tggtagttgc tgatgatacc gtctttgatc accatccagt gagagagcat
     5101 accgcgcggc gcttcgagga agccaacacc tttgaactca cccgttgccg gaatgttcgg
     5161 cttcacaaag gtggtgtgat cgcctttgcc gatattggtg atcagtgcac tgtattggtt
     5221 ttgcaggata tcctgcaatt cacagcagtg aacggtacga ccaataatac ggcccagcgt
     5281 ggagtgcagc tgtgccactt ccagcgtgtt gccagtcagt ttctgataaa tcgcaacgat
     5341 ttcattcagt ttgttctggg tagattcgcg acctgccgcc agtttcacca gcatattagc
     5401 cagcggaccc acttctaccg ttttgccgta gaaagtcggt gatttcaccc aggaatattt
     5461 cccgtcgtca gaccaaccat cataagccgg aatggtggta ccttcccacg gtgcctgcgg
     5521 cgcttcgtct ttataccagg agtgcttcgc gctttcctga atgcctttga tcaggtattc
     5581 atcggaatga gaagtgatcg gacgatacga ggacagatcc gcattctcaa tgtagccgcc
     5641 cgggaacagg aagctgccgt ttttactgtc ggtcgggaat tccggcacgc tcaggtagtt
     5701 caccgcacct ttaccgcgcg tcagccattc cgggtagaac gcagcgataa ccgcagtatc
     5761 gaccttgtaa acctgctcaa caaagtcact cagcttgtcg atgaaagact tgatgtacat
     5821 caggcgctca aggttcagca cgcccagacc gtcgaggttg attgggttag cgaccccacc
     5881 taccgccagg ttctgaatgt gcggcgtttt accgcccagc agcgccacga cgcggttagc
     5941 gtcacgctgg cactccagcg cctgcaggta gtgcgctacc gcaatcaggt tcacttccgg
     6001 cggcagtttc atcgccgggt gaccccagta gccattggcg aaaataccca actgaccgct
     6061 ggcaaccaga tctttgatct tgttctgaac tttggtgaac tcttccggac tgttcaggtg
     6121 ccaggtcgaa acacctttca gcatttcgga ggctttggtt gggtcagctt gcagtgcaga
     6181 agtgatgtcc acccagtcca gcgccgaaag ctgatagaaa tgaacgatat ggtcatgcgt
     6241 ggtgtgcgca gccagaatga tgttacggat gtattgcgcg ttaaccggaa cgtcgatatt
     6301 cagcgcactt tctgccgcac gaacggaaga cagcgcgtga gtggtagtac atacgccaca
     6361 gatacgttgc acaatcatcc atgcatcgcg cggatcgcgg tttttcacga tctcttccat
     6421 gccgcgccac atggtaccgg aagcccatgc tttcgaaacg acgccatttt cgatttcgca
     6481 atcgatgcgt aaatgcccct caatacgggt taccggatca atagtaattc tctggctcat
     6541 gctttgctcg cctcatgacg attatgatcg ttttgtttta aaggaggaag tatcggcagt
     6601 agacgaatga gtacgatgta agcgcaaatc tcaatagcca caaaaccaat agaaatcaac
     6661 agttcttccc aggtcgggaa gtaggcgtaa ccgccgcccg ggttgaatgc caccagcgaa
     6721 taggtcagac gccaggttgc acaacctaac agcgcgctca gtgctgacag gaacagcatg
     6781 cgggaatcat tacgcagctt cgccacacgc agaacgacca gcgggaagag catcagcagg
     6841 acttcaatcc agaacatcac ggagtagaag tcaccggcaa acgctaacga cagcttgtcg
     6901 cgatagataa gctcgccaaa gcgcagcacg atgaaaatcg ccagcaacac actgatggtg
     6961 ttggtcagct tgacgaatag gctcttttca tccggaccgt tgccacgcag acccgcctgc
     7021 accagtgaac cttcaaagat gacaatcgag aagcccatga tgaacgccgt cagcagcgag
     7081 aacagcggca acatttcata gctctgccac agcggatgca ccttgtagcc cgccgagatc
     7141 atcagcgacc ccattgaaga ctggtgcatg gtcggcagca gcgcaccgag cgcgatgatg
     7201 aagaacatca ccttgttcag acgctgtagc gacaccttcc agcccagacg ttcaaacagt
     7261 gccggagcaa actccagagc catcacgccg atgtagatgg tcatacagac cgccgtctcg
     7321 aacagtaccg agttcacgtt gaagtgaccc ggaatgtaga agtacggcag gttccagtag
     7381 cgacccacgt cgatagtgat cgacaagcca cccagtgagt aaccaaacag actcgccaac
     7441 agcgccggac gcaccagcgg atggtattgc ccacggttaa agacgtatac cgcccacgca
     7501 agcgcccagc cgccacaggc aaagccggtg ccaatcagca ggtcaaacgc gatccacacg
     7561 ccccacggga agccgccgtt caggtcagag acagagccca gaccgaacac cagacgcttc
     7621 acaatcagga gcatacagat gacgattaac ggtccaaaaa tcatgaccgg tttactgatg
     7681 attttgccgc ccagcggttg tggatcatga ctcatgatcg tctcctccgt cgtgatggtc
     7741 gtttttggtg ttgcgacgaa ccagcacggt caagcccgcc agcacagcca gtggtagcat
     7801 catgccttta tacagggtgt gttgaatatt ttcggaacgc gcaccggtag aaagatcgtc
     7861 cagtttcggc aggtcgagat tttcataagg cacacccgtc agtaccagaa cctgagtacc
     7921 gccgccctct ttctcgccgt acagatgcgg ataatatttc ggcaccgtat gcaggtaagt
     7981 gtcgccagat ttcagcgtct gacgcggata gtggtattcg ctgccaggct tcagcgccag
     8041 acgttttttc gcctccgcca tcagctcttc acgcgtacca aaaatcaccg cgcccgccgg
     8101 gcacacttct acgcagccag gcagaccgcc tttatcgaga cgttccacac ctttctggtt
     8161 gcacagctcg cacttatgca gcgcaccaaa cgggttgttg tagtcgtact tcggcacgtt
     8221 gtacggacag gcgaccatgc agtaacggca accggtgcac acgtctttgt cgtaatggac
     8281 aatgccggtt ttcggatctt ttttcagtgc agagaccggg cacacagaga cacagttcgg
     8341 atcgacgcag tgcatacact gtttcttaat gtacgcgtag ccgttctcct cctggtcttt
     8401 gttgacccct gtgccgctgg tccacacttg aatgatgtta ttggtatacg gcgacagttt
     8461 gtcgttgttc gaccaggtct gttccccttg cgggttacgt tcagggaagt tgatatcctg
     8521 acacttggtg acgcaagcct ggcagcctac gcacaaggtc gagtcgtaca acatccccag
     8581 cgatcccgga attggcgggc ggttttcagc agccgcatga ctgacagacg gcagcgcgcc
     8641 cgtcagcaat gccccgcagg aggctgcttt aataaaatta cgtctgttca cggttattct
     8701 ccccgtgagt cagcgttatc tttcttttgc tgacgaccca gttcacgcac cgccatcacg
     8761 ctgacaccgg caaccagccc aaccacaccg ccgagcaaac caatagcgcc tgcagagacg
     8821 ttgccgccct ctttagcgtt aacatccggt ttctgtgaac gcggagtctg attttcgacg
     8881 ttggcaagct gatggatgcc tttatggaag ccgatacctt cttcgttaca gccatagcac
     8941 gggtgaccaa tcgccaccgg ccacacaccg ccaacatcgc agaattgcag cgttgagcag
     9001 ttgccgtaag tttctggccc tttacagccg aggtggtaca ggcaccagcc ttcgcggtgg
     9061 ccttcatcac cgaactcttt ggcaaaacga ccagcatcga agtgcgggcg acgttcgcag
     9121 tgttcgtgaa tcagacggcc ataggcgaag gtcggacggt ttttgtcatc cagtttcggc
     9181 ggtttgccgt aagtgatgat gtgcgcaacg gtcgcgagga agttgtgcgg gttcggcggg
     9241 cagcccggaa tattgataac ggttttgcct ggcagaactt cttgcaagct gactgcgcca
     9301 gttgggttaa ctccagctgc ggcaacaccg ccccacgcag agcaggaacc gatagcgata
     9361 atggctgctg cgccttccgc cgctttgcgg atgtgatcca caatcggctc accggcaacc
     9421 atgcaataaa taccgttatc ttttaatggg atggaaccat ccaccactaa cacatactgc
     9481 cctttgtact tctcgagagc gttatgtttg ttctcttcga cctgatgacc gaaggcggcg
     9541 gaaagcactt cgtgatactc cagagagata gtctccagta cgaggttttc taccgttgga
     9601 tgcgttgcac gaagcagaga ttccgtacaa ccggtgcact cctgcgcgcc aatccagata
     9661 actggcggac gctgcgggtt agtaaccgat tcggccatct ctgcagcggc tttgctactt
     9721 aaccccatgg tggcggctaa tgctgcacaa agcttcatga aatcacgacg gttaatgccg
     9781 tgagaatgga tgagggtgtt atctccagtc atttatagtt attccgttgc gaagacctgg
     9841 catatatttt gcctcaatcg caaaatcaat aatgcgatcg atgcgccatt taccacacat
     9901 ttattatggt tatcgatatc atgatactgc ggcggggcgc taaaacgaag ggaagaagca
     9961 ttagtgtaat taattaaagc aagataatac gtatgtttga tcaattttcg aggcgataac
    10021 cggccataaa aaagcgcaac gtagaacagg aattattctc acgaaagaaa tccattacca
    10081 tctctttatc cagtccatag cggcgcgtca gaatacccgc ttcccaggcg ctaagctggc
    10141 gatctccggt cttttcgtac atccggtgtg agtagcctaa tacaaaaccg cgtttataat
    10201 cggcacagaa ttttgtcaca ttaaccgcac tgtcggcatg cgttgctttt aatcccgcca
    10261 ttaagccttt accaaaatgg ttattcatcg ttcacctcat tcgctatata tcgtaatata
    10321 tagcgatatt ttaggcaaag aacgactttt gcgcacgcta ttttttcatg cttgtttaag
    10381 acgtaatccc gtaagagata aacctatcag gagagatatt tatcgaacca tttcagtatc
    10441 ctttgccagg caagatcggc ggcagatttg tcataacggg gcgtggaatc attatggaat
    10501 ccgtgattaa cccccggata gatatacgcc tcataaacct tattattggc tttcaacgcc
    10561 gcctcgtaag caggccagcc ctcgttgatt cgggtgtcca gttccgcgaa gtggagtagt
    10621 aaaggcgctt caatcttcgc cacatcggca gtgggtgcct gacgaccata aaacggcact
    10681 gcgcaggcca gttccgggta agcgaccgct gccgcgttcg atacgccacc gccatagcaa
    10741 aatccggtaa tacccacttt gcctgtcgct tgcggatagc gttgcataaa ctcaattgcg
    10801 gcaaagaaat cattcatcag tttggttgga tcaacctgct gttgcagctc acgaccttta
    10861 tcatcatttc ccggataacc tccaacggaa tttaagccgt caggtgccag ggcgatatac
    10921 cccgccttcg ccactcgccg tgccacatct tcgatatacg gattcagtcc acgattctca
    10981 tgcaccacca ccacggctgg cgttttgccg ctcatttttg cgggtttaac cagataaccc
    11041 cgtacctcgc cgtgaccatt tggcgaagga tacgtgatgt actcagcaaa aatctccggg
    11101 tcggtaaact ctacctgagt cgccagcgca taatttggct tgagcaaatc aaacaacgct
    11161 aatgccgtca tcccgcctac cgcatacttc gccgcaaggt tgaggaactc acgtttcgag
    11221 attttcccgt gagcgtaata gtcgtagtaa tccagcaact cttgtgggaa atctttggcg
    11281 gttaaacgcg gcatcgttgc actcctcagt tggtgttttt ttaagcaaag cataagcacg
    11341 tgtttttgcc cagtttttcg tcactctgtg agccagacta cgggatacgc gctggcgaat
    11401 cgctaaacta gaaacattgt ttcgaaattg aacggtggaa aggagaggtc atggtctggt
    11461 tagcgaatcc cgaacgttac gggcagatgc aataccgcta ttgcggaaaa agcggtttac
    11521 gcctgcccgc gttatcgctc ggtttatggc acaatttcgg tcacgttaac gcgctggaat
    11581 cacagcgtgc gatcctgcgt aaagcgtttg atttaggcat tacgcacttt gatttagcca
    11641 acaattacgg gccgcctcca ggaagcgcag aagagaactt tggtcgcctg ctgcgggagg
    11701 attttgccgc ttatcgcgat gaactgatta tctctaccaa agctggctac gatatgtggc
    11761 ccggccctta cggctctggc ggttcacgta aatacctgct cgccagcctc gaccaaagcc
    11821 tgaagcgtat ggggcttgag tatgtcgata tcttttactc tcatcgcgtc gatgaaaata
    11881 cgccgatgga agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt aaggcgctgt
    11941 atgtcgggat ctcctcttac tcaccagagc ggacgcaaaa aatggtcgag ttgctgcacg
    12001 agtggaaaat tccgctgtta attcatcaac cttcgtacaa tttactaaac cgctgggtgg
    12061 ataaaagcgg cctgctggat accctgcaaa ataacggcgt gggctgcatt gcctttactc
    12121 ctctggctca gggattgctg accggaaaat atctcaacgg cattcctgaa gattcacgga
    12181 tgcatcgtga agggaataaa gttcgtggtc tgacgccgaa aatgctcacc gaagccaacc
    12241 tcaacagcct acgcttattg aatgaaatgg cacagcagcg tggacaatca atggcgcaaa
    12301 tggcgttaag ctggttgctg aaagatgagc gagtgacgtc ggtattggtt ggtgccagcc
    12361 gcgcggagca acttgaggag aacgtgcagg cgctgaataa tctgacattt agcaccgagg
    12421 agctggcgca gatcgatcag catatcgccg atggcgagct gaatctgtgg caggcgtctt
    12481 ccgataaatg acctgttaat aacgggcgcg agaaccgcgc ccgaatcaga tcagtgatta
    12541 tgacgagtca gtcggtcaag atagcccatt acaaatgcag agagcacaaa cgtcagatgg
    12601 ataatgacgt accacatcag tttgttatca gggacatttt tcgcatccat aaagacgcgc
    12661 agtaagtgaa tggaagaaat tgccacaatc gacgctgcta ctttgttttt cagcgacgtt
    12721 gcatccattt tccccagcca gttcagcttc tctttgttct cggagatatc gagttgtgag
    12781 acgaaattct cataaccgga aaacatcacc atcaccagca aaccgccaac cagcgtcata
    12841 tccaccagcg acagcaacac gaggatcaaa tctgattccg ccatcgagaa gatattcggc
    12901 agtacgtgaa taatctcctg gaagaacttc agcgccaggg caactaacgc cagcgaaagg
    12961 ccaaagtaca cgggggcaag cagccagcga gaagcataca ttgcattttc aagaaaacgt
    13021 tccatacatt cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt gagtaaacag
    13081 cgcgcaacag caaatcaaca tatccgttaa atctttgtcg cataattctg agggttaggg
    13141 atcttcctgg ttcccttatc aacgcatcga gagcgtctac ccttaaagga cgttgaacct
    13201 gaagggagaa aaacgatgtc tcatttaaaa gacccgacca cgcagtatta cactggtgaa
    13261 tatcccaaac agaaacaacc gacgccaggc atccaggcga agatgacacc ggtaccggat
    13321 tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc cctggtgaca
    13381 gggggcgatt ccggaatcgg tcgcgctgcc gccatcgctt acgcgcgtga aggggctgac
    13441 gtggcgatca gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa aaagatcatt
    13501 gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt taagcgatga gaaatttgcc
    13561 cgttcgctgg ttcacgaagc gcataaggcc ttaggtgggc tggatattat ggcgctggtc
    13621 gccgggaaac aggttgccat tcctgatatt gcagacctca ccagcgaaca gtttcaaaag
    13681 acctttgcca ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat ccccctgcta
    13741 ccgaaaggtg caagtattat caccacttcg tcaatccagg cataccagcc aagcccgcat
    13801 ttactggact atgcagctac gaaggcggcg attctgaact acagccgagg cctggcaaaa
    13861 caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc caggcccgat ctggacagca
    13921 ctgcaaattt ccggcggaca aacgcaggat aagatcccgc agtttggtca gcaaacgccg
    13981 atgaaacgtg cggggcaacc ggcggaactg gcccctgtat atgtttatct ggcaagtcag
    14041 gagtcgagct acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca tttaggttaa
    14101 aaaaatgccc ggttgtgaaa agcaaccggg catcattgtg aattcactct tacctgatgt
    14161 ggcgaaaacg ccttatccgc ctacaaatgc atgaagattc aacatgttgc aatagatttt
    14221 gtaggcctga taagcgtagc gcatcaggca ttgagcacct aatgccggat gcggcgtgaa
    14281 cgccttatcc ggcctacaaa cgcacgaaga ttcaacatgt tgcaatagat tttgtaggcc
    14341 tgatgaacgt agcgcatcag gcaattctac tttacttcgc tttggcggtt tcttcgccga
    14401 ccagacctat cttcaggtaa cccgcctgat gcagcgtgtc cattaccttc atcaacgtct
    14461 cgtaatcgac agttttatcc gctcggaaga agatggtggt gtctttcttg ccttcggtta
    14521 acgcattcaa cgccgtaatc attgtttcat cggtgaccgg atcgttaccg ataaacatcg
    14581 agttgtctgc cttcaccgac agataaaccg gtttttccgg gcgcggctgc ggcgtgctgg
    14641 tagaagcagg caagttaacc ttcacatcta ccgtcgctaa cggtgccgcc accataaaga
    14701 taatcagcag gaccaacatc acgtcgataa acggcgtcac gttgatatca tgcatttcgc
    14761 cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg acattatcct gcgcgtaatt
    14821 tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc cagatccagg tcacggcttt
    14881 gcagcaacaa tacctgcgct gcgacatcac ccagcatcgc tttaaagccg ccaatctgac
    14941 gcgcgaatac gttatagata acgaccgccg gaatcgctgc caccagaccg attgccgttg
    15001 ctaacagagc ttctgcgata cccggcgcaa cgactgccag gttagtggtc tgcgtttgcg
    15061 cgataccaat aaagctgttc atgatgcccc agaccgtacc aaacagccca acgaacggcg
    15121 aaatcgcgcc gatggttgcc agatagccgt taccgcggcc catttgacga cctactgcgg
    15181 cgacccgacg ctccagacgg aagctggtac gttctttaat accttcgtta tcgtcgctgc
    15241 cttctgacag ttccagctca ttctgcgctt cattgagcaa atgcaggctt aagcttttgc
    15301 taccaaaatc agcggcgata tcgttggcct ggtttaagga acgcgcttca gccagcagtt
    15361 gctgctcgcg cttaaggcga cgcttctgat taaagaactc tacgctctta ctgaagaaga
    15421 ttgcccaggt gactacggag gccaaaataa gcccaatcat cacgcactta acgacaatat
    15481 cggcgtgctg atacataccc cagacggaaa ggtccgtctg cattaaatta ttacccactg
    15541 tgtatctcca ggacgcaagt cacaaaatct gcgcataata atatcaaaac gacgtcgaat
    15601 tgatagtcgt tctcattact atttgcatac tgccgtacct ttgctttctt ttccttgcgt
    15661 ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt tatgccaatt
    15721 cttcaggatg cgcccgcgaa tattcatgct agtttagaca tccagacgta taaaaacagg
    15781 aatcccgaca tggcggacaa aaagcttgat actcaactgg tgaatgcagg acgcagcaaa
    15841 aaatacactc tcggcgcggt aaatagcgtg attcagcgcg cttcttcgct ggtatttgag
    15901 agtatggaag ccaaaaagca cgcgacgcgc aatcgcgcca atggcgagtt gttctatgga
    15961 cggcgcggaa cgttaaccca tttttcctta caacaagcga tgtgtgaact ggaaggtggc
    16021 gcaggctgcg cgctatttcc ctgcggggcg gcagcggttg ctaattcgat tcttgctttt
    16081 gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct atgaaccgag tcaggatttc
    16141 tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt ggtttgatcc gctgattggt
    16201 gccgatatcg ttaagcatct gcagccaaac actaaaatcg tgtttctgga atcgccgggt
    16261 tccatcacta tggaagtcca cgacgttccg gcgattgttg ccgccgtgcg cagtgtggtg
    16321 ccggatgcca tcattatgat cgacaacacc tgggcagccg gtgtgctgtt taaggcgctg
    16381 gattttggca tcgatgtttc tattcaagcc gccaccaaat atctggttgg gcattcagat
    16441 gcgatgattg gcactgccgt gtgcaatgcc cgttgctggg agcagctacg ggaaaatgcc
    16501 tatctgatgg gccagatggt cgatgccgat accgcctata taaccagccg tggcctgcgc
    16561 acattaggtg tgcgtttgcg tcaacatcat gaaagcagtc tgaaagtggc cgaatggctg
    16621 gcagaacatc cgcaagttgc gcgagttaac caccctgccc tgcctggcag taaaggacac
    16681 gaattctgga aacgagactt tacaggcagc agcgggctat tttcctttgt gcttaagaaa
    16741 aaactcaatg atgaagagct ggcgaactat ctggataact tcagtttatt cagcatggcc
    16801 tactcgtggg gcgggtatga atcgttgatc ctggcaaatc aaccagaaca tatcgcagcc
    16861 attcgcccac aaggcgagat cgattttagc gggaccttga ttcgcctgca tattggtctg
    16921 gaagatgtcg acgatctgat tgccgatctg gacgccggtt ttgcgcggat tgtataacat
    16981 tgccactttt ggacaatttt gcagacattt tattgtgaaa agtcttaaat tgttgcgtcc
    17041 gggatcaagg cgtcccggac gattcaggag tacaataggc agataaaggc ttaaacgctg
    17101 ttccacagga aagtccatgg ctgttattca agatatcatc gctgcgctct ggcaacacga
    17161 ctttgccgcg ctggcggatc ctcatattgt tagcgttgtt tactttgtca tgtttgccac
    17221 gctgttttta gaaaacggcc tgctgcccgc ctcatttttg ccaggcgaca gcttgttgat
    17281 attggctggc gcattgattg cccagggggt tatggatttt ctgcctacga ttgcgattct
    17341 gaccgccgca gcaagtctgg gctgctggct aagttatatt caggggcgct ggttagggaa
    17401 taccaaaacg gtgaaaggct ggctggcaca gcttcctgct aaatatcacc agcgcgccac
    17461 ctgcatgttt gaccgccacg gtctgctggc gctgctggct ggacgttttc ttgcatttgt
    17521 ccgtacgctg ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc gtttccagtt
    17581 tttcaactgg ttaagcggat tgctgtgggt cagcgtggta accagtttcg gctatgcctt
    17641 aagtatgatt ccgttcgtta aacgccatga agatcaggta atgacgttcc tgatgatcct
    17701 gccaattgcc ttgttaaccg ctggcttgtt aggcacgctg tttgtggtga ttaaaaaaaa
    17761 atactgtaac gcctgacgat tttccctgtt cccggttgct gtaccgggaa cgtatttaat
    17821 tcccctgcat cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg aagtaacgtt
    17881 taaactcacg gctaaattgc gatgcgcttt catagccgac gcgcatcgct gctgcgctgg
    17941 ccttcatgcc gtcatggatg atcatcatcc gcgccttatg cagacggtaa ttcttcaaat
    18001 actgcaacgg cgaggtgctg gtgacagact taaaattatg gtggaacgcc gatacgctca
    18061 tattagcttc tgccgccagt tgctcgacgc tcaggttttc ggtgtattta ttctcaatcc
    18121 gtttcagcac gcggctaatc agactgaagt gagtctggcg actgaccagc gccagtaacg
    18181 cgccgccgca aggtccggtc agcacgtagt acagaatttc gcggatgatc tgtttgccga
    18241 gaatacgcgc atccagtggt cgcgccatca cgtcgagtaa ccgctccgcc gcgcataaaa
    18301 tctcttctga taacgtggcg gagttaatcc cgctggctgc catcgacggc tggaaatgct
    18361 catcttcgcc aatatccatc aacagttcct gtaactgcaa aatatcgaca ttgagacgca
    18421 accctgccag cggcacctct gacgtggcat aggtttcgca ctcaaacggc aacggcaccg
    18481 tcagcagcag gtattcattg gcatcataac gaaacacgcg ttcattgata taaccgattt
    18541 tatgtccgga aaagagaatt atgatgccag gctcgtacat caccggtgta cgtgcgaaag
    18601 gcgtctcgcc atacaacaaa cgcacatcgg gcaacagttc tgacaaacta ttttctttat
    18661 ttttcagttt attaacttta tccgccagca agcggcaaat ctcttcacgt ttcatatcgc
    18721 gtaatttctt aggaataatg cggcaatttg attgtgcgca attttgtagc atttctccag
    18781 cactctggag aaataggcaa gacattggca gaaatgagca ttgagagcca gggcgctggc
    18841 gatcacaatg aaaaacatca ggcagatcgt tctctgccct catattggcc cagcaaaggg
    18901 agcaagtaat gaacaacttt aatctgcaca ccccaacccg cattctgttt ggtaaaggcg
    18961 caatcgctgg tttacgcgaa caaattcctc acgatgctcg cgtattgatt acctacggtg
    19021 gcggcagcgt gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg aaaggcatgg
    19081 acgtgctgga atttggcggt attgagccaa acccggctta tgaaacgctg atgaacgccg
    19141 tgaaactggt tcgcgaacag aaagtgactt tcctgctggg ggttggcggc ggttctgtac
    19201 tggacggcac caaatttatc gccgcagcgg ctaactatcc ggaaaatatc gatccgtggc
    19261 acattctgca aacgggcggt aaagagatta aaagcgccat cccgatgggc tgtgtgctga
    19321 cgctgccagc aaccggttca gaatccaacg ccggtgcggt gatctcccgt aaaaccacag
    19381 gcgataagca ggcgttccat tcagcccatg ttcagccggt atttgccgtg ctcgatccgg
    19441 tttataccta caccctgccg ccgcgtcagg tggctaacgg cgtagtggac gcctttgtac
    19501 acaccgtgga acagtatgtt accaaaccgg ttgatgccaa aattcaggac cgtttcgcag
    19561 aaggcatttt gctgacgctg atcgaagatg gtccgaaagc cctgaaagag ccagaaaact
    19621 acgatgtgcg cgccaacgtc atgtgggcgg cgacgcaggc gctgaacggt ttgattggcg
    19681 ctggcgtacc gcaggactgg gcaacgcata tgctgggcca cgaactgact gcgatgcacg
    19741 gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat gaaaaacgcg
    19801 ataccaagcg cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc actgaaggtt
    19861 ccgacgatga gcgtattgac gccgcgattg ccgcaacccg caatttcttt gagcaattag
    19921 gcgtgcctac ccacctctcc gactacggtc tggacggcag ctccatcccg gctttgctga
    19981 aaaaactgga agagcacggc atgacccaac tgggcgaaaa tcatgacatt acgctggatg
    20041 tcagccgccg tatatacgaa gccgcccgct aagcttttta cgcctcaaac tttcgttttc
    20101 gggcatttcg tccagactta agttcacaac acctcaccgg agcctgctcc ggtgagttca
    20161 tataaaggag gaacgtatgg ctaatccaac cgttattaag ctacaggatg gcaatgtcat
    20221 gccccagttg ggactgggcg tctggcaagc aagtaatgag gaagtaatca ccgccattca
    20281 aaaagcgtta gaagtgggtt atcgctcgtt tgataccgcc gcggcctata agaatgaaga
    20341 aggtgtcggc aaagccctga aaaatgcctc agtcaacaga gaagaactgt tcatcaccac
    20401 taagctgtgg aacgacgacc acaatcgccc ccgcgaagcc ctgctcgaca gcctgaaaaa
    20461 actccagctt gattatatcg acctctactt aatgcactgg cccgttcccg ctatcgacca
    20521 ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa gagggattaa tcaaaagcat
    20581 cggcgtgtgc aacttccaga tccatcacct gcaacgcctg attgatgaaa ctggcgtgac
    20641 gcctgtgata aaccagatcg aacttcatcc gctgatgcaa caacgccagc tacacgcctg
    20701 gaacgcgaca cacaaaatcc agaccgaatc ctggagccca ttagcgcaag gagggaaagg
    20761 cgttttcgat cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa ccccggcgca
    20821 gattgttatc cgctggcatc tggatagcgg cctggtggtg atcccgaaat cggtcacacc
    20881 ttcacgtatt gccgaaaact ttgatgtctg ggatttccgt ctcgacaaag acgaactcgg
    20941 cgaaattgca aaactcgatc agggcaagcg tctcggtccc gatcctgacc agttcggcgg
    21001 ctaacgtgca aattctcccg gtggcggtaa tgttccgcta ccggactttt cagaaatcat
    21061 ttattcccct cgcgtcccgc ccgttgttac tcttccttgt tcaggaatgc caaatataag
    21121 gacatcatca tgcagagccg gaagctctta aaagaacaac tcatctatat ccgggataaa
    21181 cgcaacggag aggtgaaaaa cagatgaaaa taatacttct gtttttagca gccctggcaa
    21241 gttttaccgt gcacgcacag cccccctcac agaccgtaga acaaacagtc cggcatattt
    21301 atcagaacta taaatcagat gccactgccc cttattttgg tgaaaccgga gagcgtgcga
    21361 taacttctgt gcgtattcaa caggcgctta ccctgaacga caatcttacg ctgccgggta
    21421 atattggctg gattatgatc cggtttgtga ttgtcaggat tttggcgatc tggtgctaga
    21481 aagcgtagcg ataaccgaaa ctgacgccga tcatgccgat gccgttgtgc gctttcgtat
    21541 ctttaaagat gataaagaaa agaccacgca gacactgaaa atggtggcgg aaaatggtcg
    21601 ttgggtcatt gacgatattg tcagcaatca tggcagcgtc ttacaagcag ttaatagcga
    21661 gaatgaaaaa acgctggccg ctttagcttc gttgcaaaaa gaacagccgg aagcctttgt
    21721 tgccgaactc tttgaacata ttgccgatta tagctggccg tggacgtggg tggtttccga
    21781 ctcttaccgc caggcggtca atgccttcta taaaaccacc ttcaagacgg ccaacaatcc
    21841 cgatgaagat atgcaaatag aacggcaatt tatttacgac aatccgatct gttttggcga
    21901 agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg gagaaaaccg ccgattccgc
    21961 ccgcattcat attcgtttta cgctgaccaa tggcaacaac gaagagcaag aactggtttt
    22021 acaacggcgc gaaggtaagt gggaaatcgc tgattttatc cgcccgaaca gcggcagcct
    22081 acttaagcag attgaggcaa aaaccgccgc cagattaaag caatgagctg aattaaatag
    22141 caattagccg gaacaataaa aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    22201 tgttgttctg gcaacctgcg tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct
    22261 ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
    22321 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    22381 tgcacaggac tattttgatt gcctacgctg cttgcgtatg tacgcagaat gataaaaaaa
    22441 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    22501 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    22561 caggcgtctg acactgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc
    22621 ggttctggcg acgagcctca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    22681 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc
    22741 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    22801 tcggtacgaa gacgtcttca ctcttataac caatcttcgc cagcgggttt ttcccggtgt
    22861 aatacatggt ggtcgagtta gccagtggcg atgggtagaa gttctgtacc tggtcgagac
    22921 ggaaacgatg ctttttcagc cacagcgcca aattcaccat atcttcatca cgcgtaccgg
    22981 ggtgcgcgga gatgaaatag gggatcagat actgctcttt acctgcctgt ttcgagtagg
    23041 tatcgaacag ctctttaaag cggtcataac tgcccatgcc cggcttcatc atcttcgaca
    23101 acggcccttc ttcggtatgt tccggagcaa tcttcagata accgccgacg tgatgggtcg
    23161 ccagttcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcaa
    23221 tcagaatctt tttaatgcct ttcagatcgc gagcgcggcg atagaggttg atcgtcggtt
    23281 cgtggttagt gtccatatgc ggacaaatat ccgggtaaac gcaggacaaa cgacggcagg
    23341 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
    23401 gatcggaaat tacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa
    23461 tgatcgaatc ttcggaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga
    23521 aagagcagcc gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag
    23581 ccggaatacg ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa
    23641 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    23701 caccgtgttt ttgcatcaat gcgcgggcgc aacctgggtt agtttcgtgg tgcagaatac
    23761 gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca
    23821 cgtaggtttt ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct
    23881 gctttttcgg tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg
    23941 ggattgggtc gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag
    24001 gcagcgcctc tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct
    24061 cgcccatcgc cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca
    24121 gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat
    24181 aatgcgcggt acggcgcaga ctagcctcaa taccaccgag gatcaccggt acatctttcc
    24241 acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga
    24301 cgttatccgg cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga
    24361 tcatcgaatc catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca
    24421 tgaaatcgtc tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg
    24481 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    24541 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    24601 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    24661 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    24721 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaagta ttccatactc
    24781 gccctcctcg ggcgagtatg aagattacgg taccggattg actaacagtt gcccaatcga
    24841 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    24901 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    24961 gcctctgtct tccgggaacg gcatcgcacc attcacgtta cggatctgga acatcacgcc
    25021 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    25081 ctgcgcggtg acatcaatac ggttgacgtc ccacagttgt ccattaatac ccgggtcatc
    25141 acccagactg atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa
    25201 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    25261 cagggtagaa accagaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct
    25321 cgccgcttcg ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc
    25381 gcggcgctcg cccggtgcca acgaaagttg cttcaccgat acaggagcag gcaggaatcc
    25441 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    25501 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    25561 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    25621 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    25681 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    25741 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    25801 cagagtagct gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat
    25861 catccgtgcc ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga
    25921 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    25981 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    26041 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    26101 cagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    26161 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    26221 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgtttccc gcaaaaatac
    26281 agattgccga cttaaacttt tccggcggct tcgcgctctg cgacttcttt atcgagctcg
    26341 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    26401 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    26461 cgattaagat taatcttatt cgaagttgtg gagacgcaca cgggaataat cgggacgccc
    26521 gccgcaattg ccgcgtgaaa tgccccagtc ttgaacggta gcaggccgcg accacggctg
    26581 cgggtacctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcactact
    26641 tccgcaatag tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    26701 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    26761 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    26821 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    26881 ccgaacagcg gcgcaagacg accaaacata tgcccaaagg tggccacatg tttcgggtta
    26941 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    27001 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    27061 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    27121 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    27181 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg
    27241 aaacagcgcc ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag
    27301 agtcgatctc aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac
    27361 gttcgccagt gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat
    27421 gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg cgccagacca tcctcgccac
    27481 gcgcggcttc tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg
    27541 gcagatcact taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat
    27601 cggaagcatc ttcaattacc accggcggca taacatgggc attttccggt aaggtgatca
    27661 aagccttacc tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac
    27721 ccgcatcgga agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg
    27781 tcgccccagg cggcaacgtt aatttgccgg tgagcggttc gccctgacca cgcgccgacg
    27841 gcagcgtaat cggatcaatg gcatagctac gaccggtgga atcaacaaac actaccggtt
    27901 ggttgctctt acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg
    27961 gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg
    28021 tgacaggttc agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct
    28081 gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca
    28141 gcaggttatt cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt
    28201 ccagctcact ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt
    28261 tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg
    28321 gtttcggttc atcttcatta cgaatgatct caatcacttc gtcgatattg agaaacgcca
    28381 ccagcaaacc ttcgaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc
    28441 ggcggcgcac ggtatcgcgg cggaacacca gccattcgga gagaatttcc agcaggtttt
    28501 tcaccgccgg acgaccatcc agaccgatca tattaaggtt aatgcgatag ctcttttcca
    28561 gatcggtggt agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg
    28621 gcacaatcac cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca
    28681 tcggcagctt tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa
    28741 cctgatgcgg caatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca
    28801 tacgcactga accacgtccg ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa
    28861 tttccgcttc agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg
    28921 ttttcggctg gtcgattaat gcgattgccg cctgagccac ttcacgcagg ttatgcggtg
    28981 gaatatcggt cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca
    29041 gacgggcagg tagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag
    29101 ccgtcccctg ccccaactcg ctcaatagca gctcggaata tttcgacaac cgggattcgg
    29161 tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat
    29221 caaccagcgg ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg
    29281 cgctatcgcc gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt
    29341 taaatttggc gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa
    29401 caggtttcag accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt
    29461 agtttaagta ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca
    29521 ttaattctga ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac
    29581 tacattatct gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct
    29641 ccgacggcga taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacataatc
    29701 ctggggatta tgcagcggaa aaccgccaac gtagggttta agcagtttga ttaacggctg
    29761 atagtagata ggaatcagcg gtgcctgctg gttgacgatc acttctgcct gctgatacaa
    29821 cgcattacgc tttgtcgcat cagtgatctg catggcctgg tttagtaagg cgtcatactg
    29881 cgcatttttc cagtgaccga cgttttcttc actatcgctt ttgagcgtgt tcaggaagct
    29941 ggaagcatca ttgtacgtcg catcccacga ctgccgagac agcatgaaat caccggctcg
    30001 tcgggcatca agataggttt tccactccat tgtgcgcagc gtcacctgtg cacccagcca
    30061 ttttttccat tcggaagaca acgctatcgc ggtcttttca tgcagatcgt acttgttgta
    30121 gaacagctca aagcgtagcg gatgagaggc gtcgtatccc gcctgtttca gcaaggcttt
    30181 tgccatcgcg acgcgctcac tcattggctt ttgcagttca tcgaacgtcg tcgcgctaaa
    30241 gccttttacc tctggcggcg tcagcgtggt tgcgggcgtt ctcaacccca gtaccttttg
    30301 cgcaataagc tgtcgatcaa ccgtaagata tagcgcccga cgcactcgca catcgttaaa
    30361 tggcggtttc tcaaggttga agttgtaata ttcgctgttc agacgcggaa taattcgtat
    30421 ctcgccaggc agtgattttt caatggcggg aatttgctgc gccggaaccc aggtgagatc
    30481 gacctctccc gcgcgatagc ggttatagcc ggtgaccgaa ttatccagcg ccagatactc
    30541 aacctgttgc aatactgtat gttgcgcatc gcggtacttt ggatttttgc gtgcagtaat
    30601 cttttcgtta actacccact gatcaagcac aaaggcaccg ttgtaaacca tgttctctgg
    30661 cttactccag ctatcgccat gtttagcgat gacatgatga ggaaccggga acagcgtcgg
    30721 ccaggcgagc atcgtcgtga accaaggaac cggctgctca agcgtaactt caagagtacg
    30781 atcatccgtc gctttgacac ccagcgatgt aacatctgct ttacccgcaa caatagccgc
    30841 ggcattgtta atgtgcgcct gtgccagata gccagcaaaa gggcttgccg ttttcgggtc
    30901 aaccgcgcgc tgccagccga ggacaaaatc ctctgccgtc agaggctgac cgtcggacca
    30961 ctgcaaaccg ctacgcagat ggaaaatata gcgcctgccg ccgtccagta tctcccagcg
    31021 ttcagcctga gcgggctgca cctggccttc accgtccatc cataccagac cttcaaacag
    31081 atccagcaca atctgcgcgg cagtattctc ctccaccttt tgcgggtcga gcgtacctgg
    31141 gtcgctatga ttgttgtaac gaaagacttg ttgcggggcg agctgtgtgt tggtgggaac
    31201 gtcagcagcg tagagaggag ccgcacttac caggctgacc agccataata aatttcgcgt
    31261 atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc tgtggcattg
    31321 ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc cccatcacgc
    31381 taacggcaca taaacattcg tttccagtcg ctgctccgtc accccttcgg caagattggt
    31441 gtagtgaaac agaatcggtg ctttacgcat tttctcgccg cttgcaggca gccagtggcg
    31501 aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc gagccacggc
    31561 ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg gaatcggttc
    31621 gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg cagggtcgtc
    31681 ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct ctttacgcca
    31741 catgataaat ttcgccacgc tgtagttgag caactcaggc ctacagcggt gcggcaacat
    31801 cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt ttgtcatgtt
    31861 gcctccctgc gaaattattc ccgggaacgc atcccgggag cggtaacaat tacggattta
    31921 ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg acttcaccct
    31981 gaatctcaac cgtatctttc ggcgtcaccg tcacgccgtt ccagcgtttg tggtcgatat
    32041 caacattgat agtaccgctg gcatctttga acacgtagag atcgtcagag atgcgttcaa
    32101 cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacag
    32161 tcgttacgct gccgttcggc ccctggaatc ctccggcctg actttgcgtt gccgatgggc
    32221 cagaaaaacc gccctgctct gctgccatca ccggtgcgct gcacagggcc attactgcga
    32281 ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta
    32341 acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttagtgc tgtacagagc
    32401 gcgttacaac acggtttact agcagcaaat acggttatcg cagggatgaa aaaatgcgaa
    32461 ttttactgat agaagatgac atgctgattg gcgacggcat caaaacgggc cttagtaaaa
    32521 tgggttttag cgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg
    32581 caccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt cgcgatattt
    32641 tgcgcgaatg gcgagaaaaa ggtcagcgtg agccggtact gatcctgacc gcgcgcgatg
    32701 cgctggagga acgtgtagaa gggctgcgtc tgggagctga cgattatctg tgtaaacctt
    32761 ttgcgttgat agaagtcgcc gccaggctgg aagctctgat gcgccgaacc aacggccagg
    32821 ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc
    32881 tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc
    32941 gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg
    33001 aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggta
    33061 gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa tgaaatttac
    33121 ccaacgtctt agtctgcgtg tcaggctgac gctaatcttt ttaattctgg cctcggtgac
    33181 ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgaattgtt
    33241 cgacacccaa ctgatgctgt ttgccaagcg gttaagtacg ctcgatctca acgaaatcaa
    33301 cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga
    33361 tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaacg atggcgataa
    33421 cggagaagat attccctata gctatcaacg ggaaggtttt gctgacgggc aactggtggg
    33481 cgataaagat caatggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt
    33541 tgttggtcag gaatgggaat accgtgaaga catggcgctg gcgattgttg ccgggcaatt
    33601 gatcccgtgg ttggtcgcac tgccggttat gttaatcatc atgatggtac tactgggtcg
    33661 tgaactcgcg ccgctgaaca aactggcgct ggcactacgt atgcgtgacc ctgactcgga
    33721 aaaaccacta aacgcgactg gcgtacccag cgaagtgcga ccactggttg agtcgctaaa
    33781 tcaactgttc gcccgcacac atgcgatgat ggttcgtgaa cgacgcttta cctccgacgc
    33841 agctcacgaa cttcgtagcc cgttaacggc gctgaaagtg caaaccgaag ttgcgcagct
    33901 ctctgacgat gatccgcagg cgcggaaaaa agcactgctc caattacatt ccgggatcga
    33961 tcgcgctact cgtctggttg atcaactgct cacgctatcg cggctggact cactggataa
    34021 ccttcaggac gtcgcggaga tcccgcttga agatctcctg caatcgtcgg tgatggatat
    34081 ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg tccagggcat
    34141 caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc tggataacgc
    34201 cgtgcgttac agtccacagg gcagcgtggt ggacgtcacg ctgaatgccg ataatttcat
    34261 cgtaagggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt
    34321 ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcga ttgtccagcg
    34381 aatcgccaag ctgcatggca tgaatgttga gtttgggaat gcggaacaag gtggatttga
    34441 ggcgaaggta agctggtaag ggtaagactt ttgctaaatt cgcaaaagac tttgcacatt
    34501 ttgctaattt caccgtaccg ctctgtgacg tactatagtc ggcaaacgtc tcaccttgag
    34561 gttaaaaaat gagcaacatc ctgattatca acggcgcgaa aaaattcgcc cactccaatg
    34621 gtcaactgaa cgacaccctg accgaagtcg cggatggcac actgcgcgac ctcgggcatg
    34681 atgttcgcat cgttcgcgcc gacagcgact acgatgtcaa agcggaagta caaaactttc
    34741 tctgggctga tgtggtgatc tggcagatgc caggctggtg gatgggcgcg ccgtggacag
    34801 tgaaaaaata cattgatgat gtattcaccg aaggtcacgg gactctgtat gccagcgatg
    34861 gtcgtacccg caaagatccg tcgaaaaaat acggttccgg cggcctggta cagggcaaaa
    34921 aatatatgct ttctctgacc tggaacgcgc caatggaagc cttcaccgaa aaagatcagt
    34981 tcttccacgg cgttggcgtt gacggtgtgt atctgccgtt ccataaagca aaccaattcc
    35041 tcggtatgga accgctgccg acatttatcg ctaatgacgt gataaaaatg cctgatgttc
    35101 cccgctatac tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa ctagagctca
    35161 ggctttagaa ggagttaacc atgcttaccg taatcgcaga aatccgtact cgtcctggtc
    35221 aacatcaccg tcaggcggta ttggatcagt ttgctaaaat cgttccaacc gtactgaaag
    35281 aagaaggttg ccacggctat gcgccaatgg tggattgcgc tgctggcgtg agtttccagt
    35341 ctatggcacc ggattctatc gtgatgattg agcagtggga aagcatcgcg catcttgaag
    35401 cgcatctgca aaccccgcac atgaaggcgt atagcgaagc cgtaaaaggt gacgtgctgg
    35461 agatgaatat tcgtattctg cagccaggga tttaacattc ctgaaaagag gccggtgttg
    35521 ctaccggcct caggtcagat tagccattct ttcttcccgt tttcagcccc cgatggaaat
    35581 cgttaattcg cttaatggtt ttaaccgcac gctgcggcac cgcggaagcc accgaccaga
    35641 caatcatttc caggcacaac aataccgtac catgtagtgg cgttttcccc ttttcatccc
    35701 cgcgcggaac atgaatcacg atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg
    35761 tcagcaaaat aacagggatc cccagccgtc tcgcttcacg cagcgttgtc agcccttccc
    35821 ggtgcgcaga tttttgcgcc atcatgatca gaacatcgcc acgttgaagc gcaatcagtt
    35881 gctcggcaag gccaataccc gtacggttta gtgccgtggc aggcaaacct atgcggttga
    35941 atagtcgtgc ggtatagtcc gccagaatac ccgaagcgcc aatgccaaaa attgcgactt
    36001 gccgtgcctg taccaacaac gctaccgctt gcgcaagcgc gtggcggttt tcaggctctg
    36061 acaggacatt acaggtatac aaatgccctt caagaacaaa gtcgattgcg gtgttaacat
    36121 cgctcgtcaa gttactcacc gtcgtagaca tcttttcgct ggaactaagg gctggaccaa
    36181 gccactgttc cagagtgcgt ttcagatcac gcagcccggc aaaccccagg gcctgtatag
    36241 ccctgataac tgtggcatcg gaagtgttca acgtggccgc aatttccatc gccgtttgat
    36301 cgagtaccac ttcacgatgc tcattaatat agctggcgac agctcttaac ccaggcgtta
    36361 actgactcgc gcgagtgcga aaacgctcac cataaatatc aacccgcgtt ttgtctttct
    36421 gaatcatttg gcctccggta aaggtcgccc cgcaatttgt gattgtatct gcgcagccat
    36481 cagccccaga gatgcaaagg agttagaaat tgcctcttca cgagaaatca gcacataacg
    36541 cccggaacga caggcagtca gaaactgaca ccatcccggc atgacttttt ccattgctgc
    36601 cagctcatcc tgaggtttac caccggtgtc accgcgccag gttgcaaaaa caaaatcggc
    36661 atccagttca ggtaaacgct ccgcgctcac atccattcgc ccaccttcag gaatgctctc
    36721 aatcagcggc ggaaagcgaa aaccagcatc gcgtaatacc cgccccagcg aatgatagct
    36781 atgcatcaca ttaatttttc cctgatttgc ctgaatcacc gataccgtta ttttttggct
    36841 gtccagcgtc gccttcagcg cattgatttg tgcctgatag cggcgttcaa gtatcgccag
    36901 ctgcgactgc gttcctgtca gttctgccag cttgcgatag atttctggcg cgcccccttt
    36961 gagatgatca atgctcaccg tgggcgcaat tttttccagt cgttcaatgg gtgtattacg
    37021 ggtcggttct gtgatgatca ggtcaggctt tgccgccaca atcgcttcaa tatcaatatc
    37081 agcggtgcca ataaaagcga tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc
    37141 tgatcgaatg aaatggctac cgtcaggccg agttcgcccg tggctggcga caggaggtac
    37201 accgagctca atcagaggaa tagtgatatc aagatcgtgt aaagagacaa ttctctttgg
    37261 atgagcagga acagtgactt tgcgccctaa atcatcagta aagacctgaa caggttctgt
    37321 cgcgcgggca aagaatgaca acagtatcag cagagaaaaa aataaacgca tataactccc
    37381 attaaaaact gttcctgcgc tgccagagca gtagcaaaaa gaatggtcca ccaatcaatg
    37441 aaataatgat tccggcaggc agttgcaaag gcaaaaaagc caaacgacca atgctatccg
    37501 ccaggatgac taacaacgcc cccgatacgg cgctccccag cagtaatgtc gtctgcccgc
    37561 cacgtagaat aaaacgtgat atatggggtg ctatcaatcc gacaaaaccg atgttaccaa
    37621 cacacgaaac gcagacggct gtcaggatta ttggtgcggc gacacgtaac aacgccagac
    37681 gagaactatt cacccctaac ccggttgcaa cctgatgacc aagcagagcg atatccgccg
    37741 tcctggcggt gaacagcaat aaaagagcgg ctggcaacat ccaacaggct gaaataccga
    37801 ctaacatcca gttagcagca tgcaggcttc ccgataacca catcaatgca gtttgtacat
    37861 ctcgaacatc tgcagtggtc ataaatacac cgataccggc ggcgaaaaac caggaaacac
    37921 caataccaat cagaacaaat cgcgggcggg aaatttcgcg tgcacaaaaa atcacgatta
    37981 aagccaccaa caagccacca gcaagaccag ccactggacg ccagaacatt cccagcattg
    38041 gaaactggaa aatcagccac agcaccgcca cgctacatcc ttctttgacg ccgattaaac
    38101 caggatcggc aaggccgtta cgcgcaatgg attgcatcgc ggcccccgcc atacctaaca
    38161 tggctccgca tagcaatgcc atgatgactc tcggcaagcg aatatcctgc acaatataac
    38221 gtgcgtctgt gcttacgttt tctggagcaa acaacgctcg tcctacttcc gatgccggga
    38281 caaaaaaaga accatgcatc agaccgaata tcagtattcc aaccgcaaac agaaacagcc
    38341 caccaagcaa caccagattt ttagggcgta ccagtgtcga aaacttacca acacgtagtg
    38401 gacgtagccc gacgttcatt atccgactca tttgaacatt ctcgtcgcca taatgacaaa
    38461 aacaggcgca ccaactaacg ccgtcatgac accggtcgct aactcatggg gcgtgaaaag
    38521 cgtccgagca ataatatctg ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg
    38581 gagcagaacg cgcaaatccg ctgataccag acgcctgaca atctgcggta ctagcagacc
    38641 gacaaaacca atggggccag caatcgatac ggctgcgcca cagagcaacg cgatcgcaag
    38701 cagagtgaat gttcgcgtgc ggagtaccga gacgcccagc ccctgcgcca tgcgatcacc
    38761 cagtgccaac atgttaagag agggcgcaag gtaaatggct aacacaaccc cccccagaga
    38821 aaaccaggcg gacgtaccca gcgtcgccca atcctgcccc gctaaatcac ctgctagcca
    38881 tgtccgcatc gccagcagtg tttgttcgtc aagaatcagg attgcagccg ttattgagga
    38941 tacgaacgcc gatagcgcca cgccgcataa agtaactttc attggcgtta gccctgaacg
    39001 ccctgcggag gaaagcagca atatcaacag gaacaataac gcgccgcccg tagatgccag
    39061 cagtgggcgt ccaaccggaa aggccagccc aagcgcactg gcagcaacga cagcaagagc
    39121 ggctccggca tttaagccta aaatatgtgg ctcccccaat gggttacgaa tgacagcctg
    39181 caacaaagca ccagcaactc caagagaagc accggtaagc aatgctgccg ctaaacgcgg
    39241 caaacggagt cttacaatta cgttgtgatc gaagttttgc ggttcaaaat gaagaaatgc
    39301 ctgcaacacc gtttgtggtg caataaatcg ggcaccaatc cctggatata ccacgcatcc
    39361 cacaagcagt aaggcgagga aaattacagt aaccagccct gtgcgcatca tactttttct
    39421 acaccacgaa acggcatgaa gaacggcttt cccgtcagag tatttattga tgcgtgaaca
    39481 tcaacgtcaa agacagcttt taccagctca ggcgtgcaat gctccccctc attcaacaca
    39541 cgcaccactt tcccctggcg taaaaatatc aacgtatcac cataattgac ggcaaaattc
    39601 agatcatgca acaccacgac gactgtacgc ccgtgatgac gcgttaaatc atgtaataac
    39661 tcaagaattt caacctgata acgtaaatca agccaggttg tgggttcatc cagtaggata
    39721 taaggcgtct tttgcgccaa caccatcgca atccagcaac gctggcgctg cccaccagag
    39781 agtttttcaa caggtaaatg agcaaactcc tgcgtaccgg ttaatttcag ggcctcttct
    39841 accgcctgct cgtcggcgtc gctccattga cgtatgaaat tttgccaggg aaaacgccca
    39901 cgcgacacca gttcataaac ggttaagccc tccggcaata atggcgactg cggcaagatc
    39961 ccaagtctac gggataacgc tttcgttggt tgctcgtgaa tcgctttacc atccagcaat
    40021 attgagccgc ccatgggttg caagacacgt gcaatggtgc tgagcaacgt cgactttcca
    40081 cagccgtttg ccccaaccag aacggtcatt ttttctgtgg gaacggagaa agaaacacca
    40141 tcaacaatga ttttcttgtg atacccggca gaaacattgt cgaggatcag cccctgccct
    40201 tttgcgttat gtgccacgtg tacgccaact taatcaaaga aaaacaaaaa taaaactcat
    40261 tctcttttga gagtttgacg c
//
LOCUS       DBFIFS010000019        39992 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.019, whole genome shotgun sequence.
ACCESSION   DBFIFS010000019 DBFIFS010000000
VERSION     DBFIFS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.019"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1218)
                     /gene="ccp"
                     /locus_tag="N7A07_000933"
     CDS             complement(<1..1218)
                     /gene="ccp"
                     /locus_tag="N7A07_000933"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU2475652.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRN"
     gene            1623..3272
                     /locus_tag="N7A07_000934"
     CDS             1623..3272
                     /locus_tag="N7A07_000934"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU2475653.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3323..3925)
                     /locus_tag="N7A07_000935"
     CDS             complement(3323..3925)
                     /locus_tag="N7A07_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU2475654.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4445..5344
                     /locus_tag="N7A07_000936"
     CDS             4445..5344
                     /locus_tag="N7A07_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2475655.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5393..6406
                     /gene="yhjD"
                     /locus_tag="N7A07_000937"
     CDS             5393..6406
                     /gene="yhjD"
                     /locus_tag="N7A07_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU2475656.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            6818..8140
                     /locus_tag="N7A07_000938"
     CDS             6818..8140
                     /locus_tag="N7A07_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2475657.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8322..10382)
                     /locus_tag="N7A07_000939"
     CDS             complement(8322..10382)
                     /locus_tag="N7A07_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU2475658.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10452..11219)
                     /gene="pdeH"
                     /locus_tag="N7A07_000940"
     CDS             complement(10452..11219)
                     /gene="pdeH"
                     /locus_tag="N7A07_000940"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU2475659.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11451..12380
                     /gene="kdgK"
                     /locus_tag="N7A07_000941"
     CDS             11451..12380
                     /gene="kdgK"
                     /locus_tag="N7A07_000941"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU2475660.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12476..13972)
                     /locus_tag="N7A07_000942"
     CDS             complement(12476..13972)
                     /locus_tag="N7A07_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU2475661.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14193..15479)
                     /gene="dctA"
                     /locus_tag="N7A07_000943"
     CDS             complement(14193..15479)
                     /gene="dctA"
                     /locus_tag="N7A07_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU2475662.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15662..17650)
                     /gene="hmsP"
                     /locus_tag="N7A07_000944"
     CDS             complement(15662..17650)
                     /gene="hmsP"
                     /locus_tag="N7A07_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU2475663.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17732..21205)
                     /gene="bcsC"
                     /locus_tag="N7A07_000945"
     CDS             complement(17732..21205)
                     /gene="bcsC"
                     /locus_tag="N7A07_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU2475664.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21187..22293)
                     /gene="bcsZ"
                     /locus_tag="N7A07_000946"
     CDS             complement(21187..22293)
                     /gene="bcsZ"
                     /locus_tag="N7A07_000946"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU2475665.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22300..24675)
                     /gene="bcsB"
                     /locus_tag="N7A07_000947"
     CDS             complement(22300..24675)
                     /gene="bcsB"
                     /locus_tag="N7A07_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU2475666.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24686..27304)
                     /gene="bcsA"
                     /locus_tag="N7A07_000948"
     CDS             complement(24686..27304)
                     /gene="bcsA"
                     /locus_tag="N7A07_000948"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU2475667.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27301..28053)
                     /gene="bcsQ"
                     /locus_tag="N7A07_000949"
                     /pseudo
     CDS             complement(27301..28053)
                     /gene="bcsQ"
                     /locus_tag="N7A07_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            complement(28065..28253)
                     /gene="bcsR"
                     /locus_tag="N7A07_000950"
     CDS             complement(28065..28253)
                     /gene="bcsR"
                     /locus_tag="N7A07_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU2475668.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28526..30097
                     /gene="bcsE"
                     /locus_tag="N7A07_000951"
     CDS             28526..30097
                     /gene="bcsE"
                     /locus_tag="N7A07_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU2475669.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30094..30285
                     /gene="bcsF"
                     /locus_tag="N7A07_000952"
     CDS             30094..30285
                     /gene="bcsF"
                     /locus_tag="N7A07_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU2475670.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30282..31961
                     /gene="bcsG"
                     /locus_tag="N7A07_000953"
     CDS             30282..31961
                     /gene="bcsG"
                     /locus_tag="N7A07_000953"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU2475671.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32048..32155)
                     /locus_tag="N7A07_000954"
     CDS             complement(32048..32155)
                     /locus_tag="N7A07_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2475672.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            32631..33902
                     /locus_tag="N7A07_000955"
     CDS             32631..33902
                     /locus_tag="N7A07_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU2475673.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            complement(33932..34936)
                     /gene="dppF"
                     /locus_tag="N7A07_000956"
     CDS             complement(33932..34936)
                     /gene="dppF"
                     /locus_tag="N7A07_000956"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU2475674.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(34933..35916)
                     /gene="dppD"
                     /locus_tag="N7A07_000957"
     CDS             complement(34933..35916)
                     /gene="dppD"
                     /locus_tag="N7A07_000957"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU2475675.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(35927..36829)
                     /gene="dppC"
                     /locus_tag="N7A07_000958"
     CDS             complement(35927..36829)
                     /gene="dppC"
                     /locus_tag="N7A07_000958"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU2475676.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(36839..37858)
                     /gene="dppB"
                     /locus_tag="N7A07_000959"
     CDS             complement(36839..37858)
                     /gene="dppB"
                     /locus_tag="N7A07_000959"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU2475677.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(38009..39616)
                     /gene="dppA"
                     /locus_tag="N7A07_000960"
     CDS             complement(38009..39616)
                     /gene="dppA"
                     /locus_tag="N7A07_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU2475678.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
BASE COUNT         9386 a        10979 c        10067 g         9560 t
ORIGIN      
        1 gttacgtaaa ccgggtactt tctgacgcaa tttatcacgc tcttctttag tcacattcat
       61 acgaccaata tccgccgccg taatttcccc aaagttaaag tcttttttca gccccaacgg
      121 ttcgaaggaa cgtccgccga gaataattcc accatgacaa gttgcacatt tattatcttt
      181 aaataattga tagccttttt tctgttgcgc cgtcagcgca ttttcatccc cacgcaacca
      241 tttatcaaat ggggaatccg gcgtaattaa tgttttctca aattcagcaa tggcatcagt
      301 aatattttcg ccactgaaac cttgcggata gacgtcgagg aactgcgctt taagctgcgg
      361 atctttttcc agcttagcaa taatttcgtc ccaggatttt gacgccattt caatcgggtt
      421 caacggcggt ccaccagcct gatcctgcaa tgttgccgca cgaccatccc agaactgctc
      481 aacgttaaat actgagttaa ataccgtcgg cgcgttaatc ggcccaactg cgccaccaac
      541 accaatcgat gtttttctgc catcgacgcc ccccgcattc aacgcatggc aatgagcgca
      601 tgaaatggtg ctatcagccg ataaacgggg atcgtgatac agcgcaaagc ccaacgccac
      661 tttttgcgca tcggtaggca gtttttgcgg gatgggctgc accggttcat tgcgatgctc
      721 cggagcagta tcattgctgg cgtaatattc cgcgcgctgt tttgcaatcc aggccagtat
      781 ttccgcccgc tcttcatcac tcaccttacc cgcccagtgt agcgcggtat aacgcgttgg
      841 cggcatagtt tcatactgca tcacccattc aatcttattc agatcgcttt gcgaaacggg
      901 tttatcagcc agcagagcag cacgcacggc ctcgaggtta aaagatttat atccaagctt
      961 aatgtcgtaa tccatcaact gtttcgcgcc aggaatataa taataggcgg gtaattctgc
     1021 cgaaggcgtg tggcaatagt cgcatccttt ttcgcggaga aagcctaaaa ccttattatt
     1081 ttcactgaca gcagatgcct gaacatcggc ctgtttactg cgtttattat cgtggtacca
     1141 cacataacca gataacccta aatagcaaat cgcgacgcca gccaggccga tcgcggtaat
     1201 acgtgagacc attttcatta ttcttccctc acggtggtga gttattgtga cccagaaaat
     1261 tttccagaat gctgcaaacg tctctgcgag acaaagccat cataaaaaac agggagatac
     1321 gctttttgat aggaatcaat caattacatg aatataaact atcaataaga tagcctgtgc
     1381 ctattaccca gatcaggaat ggcatatcat gattgcggca agagcaggat tgaatgttct
     1441 gaaaatgaaa aacaacacga aatctgtatt tttataaatt ccattgatat tagtgcgtta
     1501 tattttttga caggttaata aacgtgatcc accgcacgct ttgtcgccca ccaggcggag
     1561 cgaatgacta cccttaaaga aaagcccgat aattagcgac gaatttcgga ggttggatcc
     1621 ttatgctcaa tcagaaaatt caaaacccta atccagacga actgatgatc gaggtcgatc
     1681 tctgctatga actggacccg tatgaattaa aactggatga gatgatcgag gcagaaccgg
     1741 aacccgagat gattgaaggg ctgcccgcct ctgatgcgct gacgcctgcc gatcgctatc
     1801 tcgaactgtt cgagcatgtt cagtcggcga aaattttccc cgacagtaaa acctttcccg
     1861 actgcgcacc caaaatggac ccgctggata ttttaatccg ctaccgtaaa gtgcgccgtc
     1921 atcgtgattt tgacttgcgc aagtttgttg aaaatcactt ctggctgccg gaggtctact
     1981 ccagcgagta tgtatcggac ccgcaaaatt ccctgaaaga gcatatcgac cagctgtggc
     2041 cggtgctaac ccgcgaacca caggatcaca ttccgtggtc ttctctgctg gcgctgccgc
     2101 agtcatatat tgtcccgggc ggccgtttta gcgaaaccta ctattgggat tcctatttca
     2161 ccatgctggg gctggcggaa agtggtcggg aagatttgct gaaatgcatg gccgataact
     2221 tcgcctggat gatcgaaaac tacggtcaca tccccaacgg caaccgcacc tattatttga
     2281 gccgctcgca accaccggtt tttgcgctga tggtggagtt gtttgaagaa gatggtgtac
     2341 gcggtgcgcg ccgctatctc gaccacctga aaatggaata tgccttctgg atggacggtg
     2401 cagaatcgtt gatccctaat caggcctatc gccatgttgt gcggatgccg gacggatcgc
     2461 tgctcaaccg ttactgggac gatcgcgaca cgccgcgtga cgaatcctgg cttgaggacg
     2521 ttgaaaccgc gaaacattct ggtcgcccgc ccaacgaggt gtaccgcgat ttacgcgcgg
     2581 gggcggcctc cggttgggat tactcttccc gttggctccg tgatactggt cgtctggcga
     2641 gcattcgtac cacccagttc atccccatcg atctgaatgc cttcctgttt aaactggaga
     2701 gcgccatcgc caacatctcg gcgctgaaag gtgagaaaga gacagaagca ctgttccgcc
     2761 agaaggccag tgcccgtcgc gatgcggtaa accgttacct ctgggatgat gaaaacggca
     2821 tctaccgcga ttacgactgg cgacgcgaac aactggcgct gttttccgct gccgccattg
     2881 tgccgctcta tgtcggtatg gcgaaccatg aacaggccga tcgtctggca aacgccgtgc
     2941 gcagtcggtt actgacacct ggcgggattc tggcaagcga gtacgaaacc ggtgaacagt
     3001 gggataaacc caacggctgg gcaccgttac aatggatggc aattcaggga tttaaaatgt
     3061 atggcgatga ccttctgggc gatgaaatcg cgcgcagctg gctgaagacg gtgaatcagt
     3121 tttatctgga acagcacaaa ctgatcgaaa aataccatat tgccgatggt gttccccgcg
     3181 aaggcggcgg tggcgagtat ccgttgcagg atgggtttgg ctggactaac ggtgtggtac
     3241 gccgtttaat tggtttgtac ggcgaaccat aatattttta cagccagccg ctaacttcct
     3301 gctggctgta aaattatcct cttcaggagg agatatttaa catcattgcc gcctgggtgc
     3361 gatttttcac ttccagacgg cgatacaggg attccagatg cgcttttacc gttccggtac
     3421 tgatattcag cgctctgccg atctccttat ttgattcgcc cgccgctaac atggttaaaa
     3481 tctcccgctg gcgggcactt aacgatttga gatctttaat gtccttttcc ggcgtcgtcc
     3541 gccagtctcc aggcagaaac attatcccca tcgccgcact atttaccgcc aacgcaaatg
     3601 tctcgacggt tgaatcacga ggcacaatgg ccagcacatt aaaatggata acttcctgta
     3661 accaccgttt attgcagtcc gtcgccgtaa ttaacacctt aacctcagga aattgcacca
     3721 cggttttttg cagcaaccag tagcaaaact caccatcctg atcgccatcg agcataacta
     3781 aggcttcagg gtaactttcc agcttttgcc ataactcgtc tgcctgactg gccccctgaa
     3841 tactcactcc tggaatacgc tgctgtaaac tgattttcat tccatgaata aatattgact
     3901 gcctgtcaaa catgactatt tgcataactg aatctccacc tgaatacgtt aaaaagactt
     3961 aagtagtgga agggtattac ccgcgagaaa aaataagaat tcgccatttg gcggtggcca
     4021 ttctacagag atggcgcgta gaaaatagtt accgatataa atagttacag ctaaacgcct
     4081 gaaattacat gtcgagggca ctatttaaaa caattttgag gatttcctta tattggtggt
     4141 tagtacgcat gcaattaaaa atgaaattcc gcgaccacaa gccaaaataa caaatggcaa
     4201 tgagacaaaa ataagcacaa atagccaata tgtcctctat tcactttaaa gggaatcgct
     4261 gaaaaatacg ctctgtttaa ggggattcac ctttctcaga aagctattcc gcccttttcc
     4321 tgctgagaaa tcgccacatt cgtcatgaca acattgtgaa acccggcatt agatgttaga
     4381 aaaaataata aacaatgcga tatgcgcgtt actgtttcgc tgtgaacaac aatcggtcaa
     4441 agaaatggat aaaattcacg caatgcagtt gttcatcaaa gtcgcggagc tggaaagttt
     4501 ttcccgcgca gcggatttct ttgctttgcc aaaaggaagt gtttcgcgcc agatacaggc
     4561 actggaacat caacttggca cccagcttct ccagcgcacc acgcgacggg tcaaactcac
     4621 gccagaaggc atgacctatt atcaacgagc aaaagatgtg ttgagtaatc tcaacgaact
     4681 ggacggtctg tttcagcagg atgccaccag tatcagcggt aaattacgca tcgacatccc
     4741 gccaggaatc gcaaaaagcc tgttactgcc gcacctgtcg gaatttctct atcagcatcc
     4801 gggaattgag ctggaactga gtagccatga ccgtccggta gatattcttc atgatggttt
     4861 tgattgcgtg atacgcactg gcgcgttacc ggaagatggc gttatcgccc gtcccctcgg
     4921 caaactgacc atggtcaact gtgccagtcc gcactatctg acgcgctttg gttatccgca
     4981 aaaccccgac gatctcactt cacacgcaat agtgcgttat acaccgcacc tgggtgtaca
     5041 tccgttaggt tttgaggttg ccagcgttaa tggcgtccag tggtttaagt ctggcggcat
     5101 gttgacggta aacagtagcg aaaactatct cgccgccggt cttgccggtc tggggattat
     5161 tcagatcccg cgcattgccg tgcgcgaagc cctgcgtgcc gggcggctta ttgaagtgtt
     5221 gccaggctac cgtgccgagc cgctctccct ttcgctggtt tatccgcagc gtcgggagct
     5281 ttcccggcgt gtaaacctgt ttatgcagtg gctggctggc gtaatgaaag agtacctgga
     5341 ctgaccgact atacttttta agaatgacca cgacagaagg acaaaagagc ggatgacgca
     5401 ggaaaacgag atcaaacgtc ccacccagga tctggagcac gagccgatta agcagttgga
     5461 taatagcgag aaaggcggca aagttagcca ggcgctggaa accgtcacca ccactgccga
     5521 aaaagtccaa cgccaaccgg ttattgcgca cctgattcgc gcgacagaac gctttaacga
     5581 tcggctgggt aaccagtttg gtgcggctat cacctatttc tcatttttgt cgatgatccc
     5641 gattttgatg gtgtcgtttg ccgcaggggg ctttgtgctg gcctcccatc cgatgttgct
     5701 acaggatatc ttcgacaaaa ttctgcaaaa catcagcgat ccgacgctag ccgctacgtt
     5761 gaaaaacacc atcaacaccg ccgttcagca gcgtacgact gtagggcttg tcggcctggc
     5821 ggtggcgctt tattccggca tcaactggat gggtaacctg cgtgaagcga ttcgtgccca
     5881 gtcgcgcgat gtctgggaac gctcgccgca agatcaggag aagttctggg taaaatatct
     5941 gcgtgatttt atttcgctga ttggtttgtt gattgcgctg attgtgacgc tttcgatcac
     6001 ctcggttgcc ggttcggcgc agcaaatgat tattagcgcc ctgcacctga acagcattga
     6061 gtggctgaaa ccgacgtggc ggttgattgg cctggcgatt tccatcttcg ccaactatct
     6121 gcttttcttc tggatcttct ggcggttgcc gcgccaccgt ccgcgcaaaa aagccctgat
     6181 tcgcggaaca tttctggctg ctattggttt tgaagtgatt aaaatcgtga tgacctacac
     6241 cctgccatcg ttgatgaaat ccccctctgg cgcagcattt ggttccgtgc tggggctgat
     6301 ggcatttttc tacttcttcg cccgtttgac gctgttttgc gcggcgtgga ttgccaccgc
     6361 cgaatataaa gacgacccgc gaatgccggg gaaaacgcag ccttaaaata ggccggatgc
     6421 agttcaaaac gccttatccg gcatacgaat ttattcggcc tggctccccg taggccgaat
     6481 aagatgcgcc agcatcgcat ccggctataa tgcgcacata acctcttgaa actctttccc
     6541 agagcctctt cagccatcta ttcaggagca aacaatttca tgccaactca taaccccagc
     6601 atataaatcc agttggtaac ttttatttaa cctgaaacca gttttatcca ctatttataa
     6661 aattatgtga agcatttcat agaagaaaaa tcactggcct aaacattatc ccctttttgc
     6721 ctggtttttg accatttccg cgatttgtta cacattgaaa tatcactttt gctgtgcgta
     6781 atatggctat tcgttagcca aaaaataaga aaagattatg caagcaacag ccacaacact
     6841 cgaccacgag caagaataca cgccgatcaa ctcgcgtaat aaagtccttg tcgcctctct
     6901 cattggcacg gccattgagt tcttcgactt ttacatttac gccactgcgg ccgttattgt
     6961 gtttccgcat atcttcttcc cgcagggcga tcctacggca gcaacgctac agtcgctcgc
     7021 caccttcgcc atcgccttcg tcgcgcgccc cattggctct gccgtttttg gtcattttgg
     7081 cgatcgcgtt gggcgtaaag cgacgctggt cgcctcgttg ctaacgatgg ggatttcgac
     7141 cgtggtgatt ggtctgctgc caggctatgc cacgattggt attttcgccc cgctgctgct
     7201 ggcgctggct cgatttggtc agggtctggg cttaggtggt gaatggggcg gcgcggcgct
     7261 gctggcgact gaaaacgccc caccgcgcaa acgtgcattg tacggctcct tcccgcagct
     7321 gggcgcaccg attggcttct tctttgccaa cggcactttc ttgctgcttt cctggctact
     7381 gaccgacgag cagtttatga gctggggctg gcgtgtgcca tttatcttct cggcggtgct
     7441 ggtcattatc ggcctgtatg ttcgcgtgtc gctgcatgag tcgccggtgt ttgagaaagt
     7501 cgctaaagcg aaaaaacagg tgaagatccc gctgggtacg ctgctgacca aacatgttcg
     7561 cgtaaccgta ctgggtacgt tcattatgct ggcaacctat acgctgtttt acatcatgac
     7621 ggtctactct atgaccttta gtaccgccgc cgcgccagtt gggcttggcc tgccgcgtaa
     7681 cgaagtgttg tggatgttga tgatggcagt tattggtttt ggcgtgatgg tgccagtcgc
     7741 tggattactg gctgatgcct ttggtcgccg taaaagcatg gtaatcatca ccacgctgat
     7801 catcctgttc gcgctgttcg cctttaaccc actgctcggt tctggcaacc cgattctggt
     7861 ttttgccttc ctgctgctgg ggttaagtct gatgggtctg accttcgggc caatgggtgc
     7921 gctgttacca gagctgtttc cgacagaagt gcgttacacc ggagcatcgt tctcttacaa
     7981 cgtagcgtcg attctcgggg cttccgttgc gccatatatc gcagcctggt tgcagactaa
     8041 ctacgggcta agtgcggtgg ggttatatct ggcggcgatg gctggcttga cgttaatcgc
     8101 cctgctgctg acccatgaga cgcgacatca gtcgttgtaa taagagtgaa aagcctgatg
     8161 cgctacgctt atcaggcctg aatttccata caatatattg aattcgcaag gatttgtagg
     8221 ccggataagg cgttcacgcc gcatccggca tgaacaaagc acactttgcc aacaatctaa
     8281 atctgtttaa ttgccggatg tcagacatcc ggcaattatc ttcacttctt catctgcgac
     8341 aaaatcgtcc ggcactgatt agcctcccct tcggaaggtg agatcagtgc cagcaacgct
     8401 gccgctggcg taaccagcgt tgccagtgcc gcagcaacag caccacgggc aatcaacggc
     8461 ccggctttca ccccagcctg aggattttta aacgtcccac gcacatacag cggcgaacgc
     8521 agtgtgataa tccgaatacc tttactctcc ggatcaatag tcaaatccag ctgttccgaa
     8581 gcaaaacttg ccgtgccggt aacgttaatc aacgcgttct cagtatcgaa agcaaaaatc
     8641 tgcgggcgtg ccacgccgtt ggcaatattc agattcgccg ccgcgcagtt cacccgcacc
     8701 tcatcgtcac caaatatcgc accgacaatg tagttgccga cattcagccc aacaatctcc
     8761 atcaggttgc ggctcaccag cccgtcattc atcaacagtt tcaggttgcc gttactgttg
     8821 cctaaaagtg ccgccaccga gttaccgcta ccgcgcagtt ccgcgtcacc gttcatttcc
     8881 cccagcgtct tctgcatcag ttccacatcg ggcatcagtt ctttcagttt cagtcgacga
     8941 gcctgaatat ctgcccgccc ctgcatcggc tttttatcgc cttccagatg aatattcgcc
     9001 gcaatgctgc cacccgcaat gccaaatttc agcggttgca ggcgcaagtc agcatttttg
     9061 aggatgatat gagtagaaag atcgctaatc ggcaggctac tgccatgctc aatgcgccgc
     9121 cctttgaagc gaacatcggc atccataacg tcccatttat cggtttcgaa gcggtcataa
     9181 ggcagcactt tgcccgcagg ctgaacgctt ttttcgccct tcttctgttc agaccgtttc
     9241 gacttttctg cccctttccc ggaatcaacg ccaatcaacg gtcccaggtc cgccagccgc
     9301 aattgccgcg actcgacatc accttccagt tttggtcgtg gcttgccggt ggtgtagacc
     9361 agagaaccgt ggatatcgct atcgccaatt cgcccattaa agccgcgata atcaaagacc
     9421 gacgattttt cagtgtcgat tttcgctacc agccgaccat ccgtttcaaa cggcggggta
     9481 tcgggcagca gaacgcccgt cagttcatag agatcaccca gtgaatcgcc agaaaattta
     9541 agccgtaaat cgacaccgcc catcttcatt gggtcattca cgacgccatc aaaagcaaca
     9601 cgggtattac ctgaacggaa atcagcctgt accggaaacg gcgtcccttc gccacgcagc
     9661 gccagcatac cgcctatttt tcccgtaccc gtgagcggtt caccgttata acgtccctgc
     9721 gccttcaggc caaaaacgta atcgcccacc ttttctttat ccgctttacc tttcgatcca
     9781 gtaacttcgc tgaacggcag cggcttgcct aagggatcaa caaaaatctc cagatccgct
     9841 ttgcttactt tgtcatcaat ggcgatccgc ccttgatcga aaagaatatt atccagccga
     9901 aacgaccatg ccgacggctt tgcattcgcg tctttgttat catcgttggc gagattaaac
     9961 gtccagttat tgttcttttc agagaggcga atcaggcgcg cgtcgggctt ttcgagcttg
    10021 atccacggca gccagaccgt tttggtcagc agcgccagcg gggccagcgt tgcttctacg
    10081 cgtggcaaat gcaccatcgt gacttcggga atatccggtg gattgccaag aatgatgtct
    10141 tccgcgtgta catggggcca cggcacccag ctgcgccagc cagtttcttg cttttgccgc
    10201 tcccacacca cgcccagatc gccacggata gcgaacggac gattcaactc cgcagagact
    10261 ttctggttga tggtcggttt gagtcgattc cagtcaaaag ttgcaatcaa aatgatcgcc
    10321 acgacaatca acaacaagaa agcccctgaa atcgcagcgg ttattttgcc tgccttgctc
    10381 atcgttcgct tcctcatatt cttcctgtgc cagtcctaaa gatagtccag ccaggcggaa
    10441 aatgaggcag cttatagcgc cagaaccgcc gtattcagcg tttctatcgg tgccgggcgt
    10501 gaaagaaacc agccttgtgc ggcgaatgcg ggcgagttct gtacatcacg ccactcttcc
    10561 ggcgtttcta cgccctcgac aatcaccccg cgacaatagc gattcattag atgtaaaagc
    10621 tgagaaaaga gtgtgcgtcc ttccggcgac tgacgcagca tcacaaacag ttctcgcgcg
    10681 attttgatgt agtcataacg cacttcactt agtgcagaga aatttgccat cccggtacca
    10741 aaatcatcca gccacagcgg gccaaattca cacatcgagg caaaggttga atctttcggc
    10801 agacggatat gctccaccag ttcgaaacgc agccagggaa gacgctcaat ctggcgcagg
    10861 atttttggtt gctgacgcag ggcgatgagc gtaggaccat caatattgac cgatgccagc
    10921 aggccgtgct ctataaagaa gtcggctttt tgcgccagca aatcaatctg ctctttcaca
    10981 acctccatcc gatggctgac ggtgatttca gtaaaatagc gatccggcgg caggcgttgc
    11041 gaagggttca agggatgcgt gaccgccgtt aatagctcca cggccattaa ccgcccgcat
    11101 gtttgataga tcggctgcca ggtgtaagca cgctcacact gcaaccaaaa acgccgttcc
    11161 tgcaagctct cgatgcttgc ttcagggttg cttattcgct ggataacctg ccttatcatc
    11221 aaagatgtcc tgtgtttaaa agtgatgcag cccggactcg acaaagatta tcggcgcgca
    11281 agggcagaac tttaccgcct ggccggggta aatttcccca ctccacaacg gaatctttca
    11341 ggaatacgtt ctggctcaaa aaaataatgg aacactgttt taatatggtt gaccagcaaa
    11401 ccaccacagc gcaaactaac gctaattttt tacagatcag gttcacgact atgtccaaaa
    11461 agattgccgt gattggcgaa tgcatgattg agctttccga gaaaggcgcg gacgttaagc
    11521 gcggtttcgg cggcgatacc ctgaacactt ccgtctatat cgcccgtcag gtcgatcctg
    11581 cggcattaac cgttcattac gtaacggcgc tgggaacgga cagttttagc cagcagatgc
    11641 tggacgcctg gcacggcgag aacgttgata cttccctgac ccaacggatg gaaaaccgtc
    11701 tgccgggcct ttactacatt gaaaccgaca gcaccggcga acgtacgttc tactactggc
    11761 gtaacgaagc cgccgccaaa ttctggctgg agagcgatca atccgcggcg atttgcgaag
    11821 agctggcgaa tttcgattat ctctacctga gcgggattag cctggcgatc ttaagcccga
    11881 ccagccgcga aaagctgctt tccctgctgc gcgaatgccg cgccaacggc ggaaaagtga
    11941 ttttcgacaa taactatcgt ccgcgcctgt gggccagcaa agaagagaca cagcaggtgt
    12001 accaacaaat gctggaatgc acggatatcg ccttcctgac gctggacgat gaagacgcgc
    12061 tgtggggtca acagccggtg gaagacgtca ttgcgcgcac ccataacgcg ggcgtgaaag
    12121 aagtggtggt gaaacgcggg gcggattctt gcctggtgtc cattgctggc gaagggttag
    12181 tggatgttcc ggcggtgaaa ctgccgaaag aaaaagtgat cgataccacc gcagctggcg
    12241 actctttcag tgccggttat ctggcggtac gtttgacggg cggcagtgcg gaaaacgcag
    12301 cgaaacgcgg gcacctgacc gcaagtaccg ttattcagta tcgcggcgcg attatcccgc
    12361 gtgaggcgat gccagcgtaa acgcagcata tgcaaaaggc tggataaaga gttacccctc
    12421 tatccagctc atccataaat acctgatggt tgtgctaact catcaccatc aggcattact
    12481 gtgcaggtgg aatatccgtc acttcaggat gtacgtcatc cgtggcgaca gaggtggtcg
    12541 cagcggcggt agatggggcc atgatttgat cccaggccgc ctgcaacgct ttcatgttaa
    12601 attccggctc gcctttcggc tgcagcagta ttaacgccat atcattcgac aactgctgac
    12661 gcagatcctg atttaacatc tccacagtca ggctgttaag gaaatcctgc cgcagtttct
    12721 gatactgctc cggcgcgata tcgacgacct gattttgcaa cgaacgcatc cgctggccca
    12781 tcagaatatc ggtatcagcc cgtgcatagg cggcaaacag tttctgcagc tccagtttct
    12841 tttgcgccac taacgcattg aactcttctt ccggcagacc tttatcgcga accttcgcca
    12901 gttcacgcgc caccagatta aggttgctgt tcagcttgtc gtttggtgat tcgatgttga
    12961 tggcacactg cgcacgcaga tacagcacac ggcagtcaaa tccaagaccg atgtctttgc
    13021 tgttactggc gcttaacgct tgctgaacat gccagaacag cgcctcacgg gccaggtccg
    13081 cacgccagta gcgcagcagt gcggctgatt cacgaatcgg ctgccacggc gtatcccaca
    13141 tgatagataa ccggtcctga cgcaccgcgt cagtcataat actcaccgct tccgcacgca
    13201 gcggagaaag cgtcggcacc ggagccggtg tttcacgttt gcctttcagt tcgccaaacg
    13261 ttttgttgat ttggtcgacg accgagcgcg catccacgtt tcccaccacc agcagcgtca
    13321 ttgcatccgg ggtgtaccat ttctgataga aatctttaat tttttccgct tcaacgggtt
    13381 gtttcagcgg atcggcagga tcgtgaccta acaaggttga ccctttcaga cgatagcgcc
    13441 accagccctc tttagtatcg gcaggccagg ttgccaccat gtcctgactt tgcagcgcgt
    13501 ggttgatggt ttctggcgtg atagtcagtt tgccagtggc atttgccaga taagagagcg
    13561 cctctttcag caaatcgtta cggttattgg gcaaactcag attaaacagc gtggtgtcat
    13621 aagagacaat taccggcggc atcgggcgtt tagggtcgat cccctgctgc cacaatgaac
    13681 gcgcctgtgc tgcgtcaagg ccaccgcttt gcgttagcgc aatacgaggg atggcgtgac
    13741 tgtaaccgct ctgttgtgta ctttcggcga gcgaaccggt attaaccagc aggcgaattt
    13801 caacacgatc gctgggacgc tggggggtgg tcagcacttg ccactgtaaa ccgttggaaa
    13861 gcgtcccctg ttgccatgct ggatcaggct ggagcgcatc tgcctgcaca tagccagcag
    13921 tggccatcat cagcaaaccg cccgctaaaa gtcgaatttt tgtgccctgc atgtgaaccc
    13981 ctgatcaact atcctggtaa taaaaaaact gcccgttgtc ggtcagtcta aatgacgtta
    14041 aaaacacttc gtgttagacc gcaagaacat gaaaatgtca cggaagaagt gaaataaacc
    14101 tgaactcacc caggtggtaa gttcaggcac aggggtcaat tatgcgcaaa cacccgcact
    14161 cggggaaggg agtgcgggca taagtgatga gattaagagg ataattcgtg cgttttgcca
    14221 tccggcgcac gattattcag cgcatcgtcc agttttttgt ggtccagttc tttcacccac
    14281 ttagcaacga caatggtcgc tacgccgtta ccgaccaggt tagtcagcgc acgagcttct
    14341 gacataaagc ggtcgatacc gaggatcagc gccagacccg ctaccggcaa atggcccacc
    14401 gcagagagcg tcgccgccag cacgataaag ccactacccg ttacccctgc cgccccttta
    14461 gaagaaagca gcaacacgat taacagcgtg atttggtgga cgatatccat ctgactgtta
    14521 gtggcctggg cgataaacac cgccgccatt gtcaggtata tcgatgtgcc atcaaggtta
    14581 aacgagtagc ctgtcgggat gaccagcccc accaccgatt tacggcagcc gagtttctcc
    14641 atcttgtcga gcatacgcgg cagcgccgac tcggaagatg aagtccccag tacaatcagc
    14701 agttcttcac ggatgtagcg gataaatttg aagatactga aaccagtcgc tttagcgatt
    14761 gaacccaata ccagcaccac aaacaggata caggtaatgt agaaacagat aatcagctgc
    14821 cccagttgca ccagtgtgcc gacgccgtat ttaccgatgg taaacgccat tgccccgaac
    14881 gcaccaatag gtgccagacg catgatcata ttgatgatgc cgaagatgac ctgcgagaaa
    14941 ctttcgatga cgttaaaaat cagttggcct ttgctgccca gacggtggag cgcaaaacca
    15001 aacagtacgg caaacagcag cacctgcaga atgttaccgc tggcaaatgc gccaatgacg
    15061 ctcgccggga tgacatccat aatgaaggcg acaatgccct ggtctttcgc ctgatcggcg
    15121 taaaccgcta ccgctttcgc atcaagcgtt gccggatcga cgttcattcc ggcaccaggc
    15181 tgcacgacgt taacgatgat aagaccaata atcagcgcga tggtactgac aatttcaaag
    15241 taaagcagtg cgactgcgcc ggtacgaccg accgccttca tgctttccat gcccgcaatg
    15301 cccgttacga cggtacaaaa gatgacagga gcgatgatca tcttaatgag cttaacgaag
    15361 ccgtcgccaa gcggtttcat ttgctcgcct atttcaggat agaaatggcc aaggagaata
    15421 ccaatggcta tcgctgtcag gacctgaaag taaaggcttt taaacagaga ggttttcata
    15481 gggtgtcctt tagtaaaacc acaggtcttg taaggttatg gggtacctgc ggccttaaaa
    15541 taacacccag acaacatcac agaaatgtac ctggatcata attgaaacaa aaaggttaaa
    15601 aagtttgagc tggctcgcac aaaccagcac tttttaaagt tttgtaatca gtttggggta
    15661 gctacttttc ttccaggtaa ctctcttcga agatttcaat agggagtgga cgagcaaaaa
    15721 ggaagccctg ggcaatacca acgcccgctt ttgccagcca gtcgcgttgt gcttcagtct
    15781 ccacgccttc ggcaatcatt tgtaagttca ggctctgcgc cagcatgata attgcagcaa
    15841 tcatgctgct atcttccggc aagccttcaa caaacatttt gtcgattttc agaacgtcga
    15901 ttggcaacga tttcatatgc tgcagctgac gcagccctgc gtagcccatg ccgaaatcat
    15961 ccagcgccac ccgaactccg gcattacgca gcggacggag gattgccacc gcagcatgag
    16021 ggtcgtcaat acgtcggctt tctgtcactt ccagaatcag tgttcccggc tgaatgcgat
    16081 agcgggttaa cagttccagc atatccgcca ccatattcgg gtgcatcagt tgcagcacag
    16141 agaggtttac cgacaagggc agcataatgc cgcgctcttg ccaggctgca agcaatcgac
    16201 aggactcttc cagcacccag tgaccgacgg taaccatcag cccacagcac tcaatgcgat
    16261 cgattaagcc atccggcagg tcccaactgc catccggttg ctggatacgc agtaacactt
    16321 ccgcactgac cagtttaccg ctggtcatct cgacctgtgg ctgtaaccaa atagcaaact
    16381 gatgattttc cagtgcatta aggatatcgc tctcttccgt caaccgcttc tgggcggctt
    16441 ccatctgctg cggatcaaag aactgaatct gattcttgcc tttatggcga gcggtaaatg
    16501 ccgcagaaat agcgcgactg taaagctgtt cggcggtgag atcgccgtag aacatcgcca
    16561 cgctaatgct acagtgcgga cggagttgaa tacgttcaat cggcaggcgc tcgctcatga
    16621 tagtgagcac ttgctgaccc aaagtgattg cgtgccacgg ttcatgtaca ccgttggcaa
    16681 tgacagcaaa gtcataaccg ctaatctgcg cgaggatcat acgtggcgac agtaccgatt
    16741 tgagtttttc caccagcgtc agcagcagaa tttctcgttg cgcctctttc agcacgcccg
    16801 cagtatcacg cagagtttca caggtgatga tcatcagcgc ggtggtttgt ttacgcgcga
    16861 caacctgctc cagcatctcc atcagcaagg ctttgttcgg caaatccgac accgggaagc
    16921 gcatcgcatt ttcgttctgt tcttcataat ggcgctgcag caattgctgg ttgaggttgt
    16981 aactgcgcac caacataccg atttcatcgt cctgatgcag acgcggtaat gccagttggt
    17041 gaccaacaag ctcctgggct gggatggcgt taagttcgcg agcaatattg cgtaacggat
    17101 gcaaaatcag gcggttaatg caccagctaa tcgccaccgt caatattaac gacaaaagta
    17161 agtaaatggt cactaacgtt gagagggtgc tcatcacgaa cttatacata cggaaggaat
    17221 ccgcctgtag caccagatag gcaattggct gcggatttgc cggacgttcg agcgagtaga
    17281 cgcccagcga gatttgaacc ggtagctcaa acaggcgagt aaccattacc ggcaccggac
    17341 gctctggaat aaaactttta cgcagcgcct ggaactggtt aggcagcact acatcggcac
    17401 ggctgaccac gcctgccggt ttaatgctgg caagaatagc ttccgcttcg ggaatatcac
    17461 ctttcaaaat ggctgaagat aaaggttcgc ggacagagcg agcaatgctt tccagttgcg
    17521 tagccgtgtt atagcgattc tgctggacca gatggaacag caaaacggtg caaaaaataa
    17581 aaacgaacac caggacaacg gctgccacca ttgccatctg cttgattgtt aacgagcgac
    17641 ttacgcgcaa attgtctccc cccggaaatc ccagattgcc gcccgagtat acccgatcgc
    17701 agcggcatta agagaggcgc tatctgaaaa cttaccagtc ggcgtaaggt atcagcggct
    17761 gcggcggtaa atccatgtca ccctgccatc cggcggcgga ataacgtacg tagagcagga
    17821 aatggctggg ggcgtaatct ttcgcctgct ggatatcaat tgccgtgccg acaaaccagt
    17881 tggaagtaac acgtcgttca agtaatgccc gcgccgtata gccgaagccc tgactgctgc
    17941 cgccatcgtt ggattgccgc gcagcttctt cctgccagtc ggtcgggatc agattcatca
    18001 gcggataacg cggcatggtt ttggtgcgtg aatgcgacca cgagccagac gcccccagct
    18061 cccacgacca gttttccgtg cgctcccgcc acatcaccgg tatggcaaac gacaggtatt
    18121 cctgcgggct gtagtaaccg ccctgaccga gtgagtagcc actcaggtct ttgtcgtaat
    18181 gccagatcat gttgttcagg ccgattgtga cgcggcgatt gttctggttg atgaccttat
    18241 agtaatagcc cgtcatccag cgcacacgcc agttatcttc gacattttta ccggttaact
    18301 ggtcgccact aagcgatgcc cagacgccgt ttgcttcacc tttatcgtag ctcaggctta
    18361 gccccacgcc gtcggcacgc acgccgcccc attttttccc ggtattgctc ggggagtctt
    18421 tttgcccacc aaaggccagc aaagaactgg agatgggacg acggtgggcg ttaacggtgt
    18481 aacccagcgg cccgatatca tcgctgtaac tgatgccgcc gaccacatcc accacgttga
    18541 agcccatcgg cgtggtgccg atatcccagc tccagacgtc atttcgccag ccgaccgcca
    18601 cgctggcacc ggaatccgac tggctgcggt tgccgctaca gtcccgtaat gtacaggtgc
    18661 cccagttgtc atcccatttg ccatcggcat tagtggagaa actgccgacg ttcatattga
    18721 cgaaatcact gcgaaagaac atccgcccgt cagaatacgg cgcatccacc tgcaacatgg
    18781 tagtgtgcgc tttcagatcg gagtaaccac cggtgccgct cgaaccccaa taatcatgct
    18841 cgagggtgac gttaagatcc tgctggcgat agaggtccgc cgcatcgctg cgcacgccgc
    18901 gtttcagcca gtcatctttc tcgtcgttac gggtcagtcg ggtaaaggtg tcgttatcct
    18961 gcggacgcgt cgtagtcaca ccggatgcga ccatggcgtc tttgtaggtt tccagcgcct
    19021 gctttggatc gcccgcctgc gcttcaaact tcgcaccgtc gcgcagcacc atcgcgcttt
    19081 ccatcgacgg tggctgagat tttgcctgcg ggatcaactt attaaacgtc tgctgcgccg
    19141 ctgcggtatc tccaagctgc gcctgcgcca gcgccacgcg ccgctgtgtg ttcagcgagg
    19201 cgttatcagt agcgggcagt ttcgccagct ggctacgtgc cgccgctgtg tcaccggcag
    19261 caatatccac ttccgtcaga ccaagaatgg catcggcgtt agttggctcc cgcgtcagga
    19321 cattctgata tgcagcgcgg gcggcggtgt aatcacgtcg ctgcagcgcc cagtcagcca
    19381 gcgtgaggtc aatacgcgtg gaaggtggtt gctgacgcag catcgcttcc gcttcagctt
    19441 ctttgccgct ttctcgcagg cgattagcgg tttccagcac ctgatcgctt tgcagtcgat
    19501 taaccagctc ctgaatattg ctgctccact gcgcacgcgg caggctgttg atatgcgcca
    19561 gcgccgctct gtcctggtca tgaccagaga ggtacagccc gtaagcgtaa acctgctccg
    19621 ggtcgttcgg cttctgctgc gccagattgc gcattaacgt atcggcctgg ctgcgttgtc
    19681 cggcctgcca gagatcctgc gaaagtcggt aagtaatcca tacgctgccg gggtccagcg
    19741 ccagtcgttg ccgctgaagt gctgccgcct gcgcccattt gccctggttt tccagtgcct
    19801 ctgcctgctg tgccagacgg tcgttttgca ggctgcgttc gatatcatca atgctacgcc
    19861 gctgactggc agagagtgag gcgataaacg cttcagcttt ttctggcgat tgctggcggt
    19921 aaatatttgc cagcccgcgc acggcgttag tgttgccgct gtccatacgc aaggtctgct
    19981 gataataacg ttcggcggcg ggataatctt ttcgcgccat cgccacatcg cccagcccca
    20041 gcactgcata actgtcggtg ttatcgacat tacgcgcctg ctggaacagg cgttctgccc
    20101 ggtcaggatt attggctttc agcgcagcat cgccctgctg gatcgccagc cagtagcggt
    20161 ttactttcag cagactgttc catttgtcgt tgttgctgct gtgcggatcc agtgcgaggg
    20221 ctttttccag attcgccact gcattggcgc gatcgccttt ctgtgaatat gcctggccca
    20281 gcgcccccag agcttcactg tctttcgggt ttgcccgcac tgcctgttgt agttcgggaa
    20341 tggctttacc cgccatacca gagtccaccg ccgctaaacc ttgcgcacga gcgcggaaag
    20401 caggatcggc cagctgtttt tgctgttctg ccagttgcga ttgcgcagcc gccacgctat
    20461 cgccatcact aaagatagaa agatattttt tcagcgccga cacactggca tcactgacgg
    20521 gcatgtcttt aatctgcccg taccagattt tagaggcccc ttcgcgcccg gcgttcgatt
    20581 tcgccatctg ttccaggacg gcaaaacctt cgtcacggcg atcgctacta aacagcaata
    20641 gcgccagatt gttttgcagg cccgtattgc ccggtgcatc cgcattgatg cgttttaact
    20701 gattaatcgc ttcgccacgg cgagccggaa ttttcgccac cgtactccag tactcgacag
    20761 caatgtcacc ttccggcggc gcaccgttga acagtttgtt gtaactcgcc acagcttctt
    20821 ccgcatgacc ggtagtcgcc tgcaatcttg cctgttgcag tgcctgacga ccatccggcg
    20881 tggaaagtag catcgtagtc cgcgacgatt tatacgcatt tgaactcggc gctaactgcg
    20941 acagccgatc gagctgtttt tgcgcgccat caatatcgcc ctgacgtaac aaagaacgga
    21001 aacgggcggc aacgacgtcc gggttattcg gatcaataag ttccagccga tacaacgact
    21061 gctgcaccag gtcttcacga tgggtcgctt cgcctaaccg aacttgctcc agcaactgtt
    21121 gctgagcggt tggtgctgcc tcgaccatcg gcatgacggc cagaccgagg gaaagcgtga
    21181 atatgtttag tgtgaatttg cgcattcctg gccccagtca ggtaataact caccttttgt
    21241 cgagaagcgg aaacggtgtt gatcccagcc ttgtccaaac agggtcagca catagttgta
    21301 ataggcatcg ctgccgggaa agttatcggc cacgcgctgg cgctgaacgg cctgcgcatc
    21361 acggttttgt aaaaagggca gcatggcggc agaaaaaccg accggtcctt taccctgcgc
    21421 tttccccgta gccacatcca ctttttccgg cggataaccg tttttctcag tgaatgtcgc
    21481 catcggttta aaccggttga gcatccgcgc tttctgcgga tcgctgtcag gcatcatgcc
    21541 tacccacatg taaacgcgga tagcgtcgta gctgctgatc aatgtttttt cggcttttag
    21601 ctgccagcct ttgtctttct catagcgcac ccagtctggc gaaaagcctt tcggggcggt
    21661 ttccagcaat aaacgttgat tggtttcgcg cagcgtagtc cacggcgcgc caaagcgggt
    21721 gaaatactgc gccagcgtcg gcggcaggta gctggggtta aaacgccagc tgttatcctc
    21781 agcaaaaccc actttgcccg gtaacaacat ggaacccagc ccaggcaccg tcaccacttc
    21841 ctcccgcgca atacgtttta gcaacgcgct gccgatgtcg gtataacgct gctctttcca
    21901 caaacgcccc gcctccagca acgaccaagc catccagaca tcaccatcgg aggccgaatt
    21961 gctgtccagc acttcccact tactgttctc tttcttgccc cacagccagg cgggcaaacg
    22021 ttcttttaaa gaaccctgag cgagattgtt ctgcgtccag tcgagaatat tatcgaaagc
    22081 tgcacggtcg ttagccgcca gggcaaagaa catgccgtaa ctttgccctt cagaggtggt
    22141 gattttgcgc gcgtcgctgg ggtcgatgac gcgcccttcc tgactgatgt aatccttttt
    22201 aaactgctcc caggcaggcc aggtacaggc tgcctgaaca ctaaaggcag ccaacagcag
    22261 catcgtcacg agtccactac gcaacacatt catcttcaat tactcgttat ccgggttaag
    22321 acgacgacgg ctgataatac gcagcaaacg ccacagtacc catgccagca atatcacact
    22381 gatagccgcc agcaccgcca gcaccgccag caccgccagc accgccagca ccgccagcag
    22441 aatcggatgg tttgccagcg cataccacaa gcgctcgaac cacggcagat gacctacgta
    22501 ataaacgtcg ccaacacgta ggctgttgat accggactcg cggatcaccg cgaccgaacc
    22561 gaacatggtg gcgcgtttgc cgctatcgtt caccgcatcg ttaagcattt cataaccgcg
    22621 tgggctatct gccagcagcg caatcacgct gcgctggtcg ttatacggcg actggaagcc
    22681 aatcaccgcc gccatcgcac cggaagaggt cagcgttgac tgggtttctg ccgcgcgatc
    22741 gctctcgtcc ggcacaatgc cggggaacgg ggtctggcgc atcggtgttt tcacccagct
    22801 ttcggtcgcc tgcaccaata ggtcgatctg cttatcgtct ttcagtttgt ccgggatacc
    22861 accgatgatc atgatgtcgg catctttgcc ctgaatggtg ctgccatcat cggtcaccgt
    22921 cagattaatc gccgggaagc ccgtctgtgc gccgataaaa ccaacagtat tcagcaacgt
    22981 ttccatctgt gcttcgttag gcgttttcgg catcacggtg atggtttgcg acagatccgc
    23041 catccggctg aacgggaagc ccgcgttagc aaaggcgcgt agatccggca tcgggatgaa
    23101 gtggtaatac ttcgagaagt cgatggtgga gtcgtcacca atcaccacat gattctgcac
    23161 cggctggaag gtaatacagt tatccaccga accgccaggc atcgggttca tatactcaaa
    23221 gtcgaagcgc agctggttgg tcgcgcccag tttcagcgcc ggaatagaga catccgtttt
    23281 gccatccagc aaaccttgta ataccggaat ccgcagcagc aggcggttcg cctcctgttt
    23341 gctgctcagg ttgaaggatt gcaggaactg gttattcagg ctgatatcca tccgcgaact
    23401 gtctttcacc ggcggcatgg tgtagcggta attaatatcc atatcaatgc cggtactgcg
    23461 catcaggtag agatctggcg gcaggtttag cgaaacatta atcgctgctg gctcaagacc
    23521 gctggattgt aactgttctt cataggtttt cagttcacca aaggtgaccg gacgatcggt
    23581 acgcacccag ttcggcgcat cgtacggctt acgcggtagc agcggtttca cttcattcac
    23641 taccacgctt tcaccacgga acagaatgtt accctgagcg atacctttcg ctgcctgcaa
    23701 caggtctttg tcgtcacgac caaacaccac cagcagtttg acgtaaggat tctgcggatg
    23761 gttaatcatc tcaatcaccg gggcttttac cgccggatga tcgcgcagga agtccggccg
    23821 tttgtcgttg gttgcaaaga caatggcatt acgatccggc agttggttat agagcaccgg
    23881 gaagttctgc ccacgccagc cagaacgcga accaaaccac gaggcgacaa tggcagatgc
    23941 ttgttgcagc ccaacatccg gcgcacccgc aaagaccatc ggcaaggtgt tggtgcggtt
    24001 atcacgcggg tcaaagaatg gcaccgggaa gtgtgacagg tcattcttca cattcagggt
    24061 ctgataggtc agatccagtc cactgctccg cccaacatcc agccaaagcg tggtgctggc
    24121 cgggttttcg cacacgtcct gataatggcc gacaaactcc agccgcacac ggttgaagtc
    24181 ggtaataaac agtggattaa tgggcatttg cgccagcgtt tttttaccca actgttcttt
    24241 ggtcactggc agcacgccca tcagctcatc attgagataa acctttaact gcgactggac
    24301 aggcagtaac gatggcgatg gcgtgtattc gaggttgagc atcgccttcg tcaccacttc
    24361 atcgctgcgc ataccaaact caatgctgcc gttcgggtta atgccacgta gcaccatgct
    24421 gcccggcggc ggcgcaattt gcgcaaaggt cagcttcaca tcacgcgaag gaccgttctg
    24481 cgccacgact ggcgcatcag cgccctgcac gcccggcatc acttgcccca cctgcggatt
    24541 ttgttccgtc tgggcggcta cagctggctc agcattgatc agtggttgcg ttgctggcgt
    24601 cgcctgcgtc atgaaagagg ggaacgcact catccccata gccactgcac aaatccagaa
    24661 tagttttctt ttcatcgcgt tatcatcatt gttgagccaa agcctgatcc gatggttgtg
    24721 ccgtttcgct ccgctccggg cggcgcggaa taaacgatac aacccaggaa accagagaag
    24781 tcagcacacg gaatatgccc ttcaccgaag aaggcgcaaa ctccgccaga tggcggtagc
    24841 cacggaagcc gagcttcaga atatccagca gactttccag cggcttatct tccgggtagc
    24901 tgtcctgcca gagcgcccat gtatccgcac gggcaaacgt acactgcaca aaatcgatat
    24961 gttgctgggt ggtgagcggc attaatttca gcccaacttc attacccatc acgcgcgcca
    25021 cctgggtcgg gaagacgtat tcctgctgac cgcgtttaag caacagattc actttctgcc
    25081 cttccagaat ctgcgcctga ccgttgatct tgatccccaa accaccgtcg gagaaatcct
    25141 gaacggtaca cgagaagagg tgaccatctt cgcgggcaat tgccgcgggc atcgtcatct
    25201 ccacgcggtg cgatcggcgt acctgtttgc tttctaccga taccgcaact gcgccgccaa
    25261 gaacaatcag gttgtagaat acccacacca tactgacgac cacggtgagc atctcggttg
    25321 gcgggccata gaagtagcgc cagatgccta ccgcaacgcc caccaggttg agcagaacaa
    25381 ggaagatgta gggccgcgag atcacccagt cgacgtactc ttcttccacc agtccacctt
    25441 tggcggtgac gttaaatttg cctttgtgcg ggttaatcag cgccaccagc gtcggtggtg
    25501 cgatatacca cgccagcacc gtttcgtaga tttcactcca gaaagagtgg cgatatttgc
    25561 cctggatctt ggagttggtc aggctggcat ggatcatatg cggcagcacg aacagggcga
    25621 tcatcaacgc tggcgcatag atgatgtagg catgaagcag caggaacgcc agcggcgcag
    25681 tcaggaagat cagccgtgga atgcccgaca agaagtggaa catggcgttg acgtagcaca
    25741 gccgctgagc aaacttcagc cctttaccgg tgagcgggtt atcgagacgg aagatttgca
    25801 ccatcccgcg cgcccagcga atacgctgac cgatatgcgc cgacagactt tcggtcgcca
    25861 gccccgccgc ctgcggaata cgcatatacg cggaggtata gccacgacgg tgcaaacgca
    25921 gagaagtatg cgcatcttca gtcacagttt cgacagcaat gccgccaatt tcatccagcg
    25981 gcttacggcg aatcaccgca caggaaccgc agaagaaagt ggcgtcccac atatcgttgc
    26041 catcctgcac cagaccatag aacagcgtgc cttcgttcgg tgttttacga aaacgcccca
    26101 ggttgcgttc aaacgggtcc ggcgagaaga aatggtgtgg ggtctgcatc atcgccagct
    26161 gtttctcttt caggaaccag cccacggtca tttgcaggaa cgatcgcgtt ggtacgtggt
    26221 cgcagtcgaa aatcgacacg aactcgcctt tggcatattt cagcgcattg ttgatgttgc
    26281 ccgctttcgc atgttcatga gtggtacggg cgatatactt cacccccacg ttttgcgcaa
    26341 actggcgaaa ctcttccctg ccgccatcat cgaggatcca gatgttcagc ttgtctttcg
    26401 gccagtcgat acccagcgag gcgtaaatgg tatttttcac cacgttgaga tcttcgttgt
    26461 aagtcgggac aaagatatcc accgacggcc acagcgacat atctttcggc aatggcaccg
    26521 gctgacgatt cagcggccat actacctgga agtagccgag caccagcaca atccacgcgt
    26581 acgtttcagc gaagagcaga ataagcccgc acaccaggct gaccggatcg tcccagttca
    26641 gcgtagaggt atatcgccac cagatataac ggcaagaaac agtcagcgac agcacaatca
    26701 acattagcgc tgagaagcgc cccggcatcc gccgtacgat cagcgctccc ccccacagca
    26761 gcatcaggaa gataaactgc gccagcgggt taaacggctg agtaacgcag attaacgcca
    26821 gaatcagcga gaaggtgacg atgataccga ggatcaaccg ccgcgccccg gcactcaaat
    26881 gaccgagctc ttttttctca tcaagatgct gtgttttatg gctaacgcgc tcaggcagct
    26941 cgttcatcca ttgatggtaa cgtccacgaa tattctgcag acctgaaaat gcccgcctgc
    27001 gcggtttcgg cgtttctttg cgcgatgcac cgatcagtaa ccagcatgtt tgaatgagat
    27061 aacggaccgg gtccagcgga cgcggacgcg aggcgttgat atgcggatac aggtttttat
    27121 gttctgcgcg aatacgctgc cagcgcgggt gctccagtgg aataaaaatc caggccagga
    27181 tcatccagaa acagccgagc gtcgcgctga aagccgacgc accgtgacga cgataatcgc
    27241 gataacgccc gataagccgc gcgttgaccg gcgggataag caaccaccgg gtcaggatac
    27301 tcatgatgca ctcccgactg gcgttttcag cccggaatag ttcaacaggc accagttcgc
    27361 cagcgtcaat atctcttcag ccgccagcgc atcactgcga tattcaccta ctggttgctt
    27421 agccgccagg cattcagcca tcgcttcatc acgatgaatg agcatcggca gtaatcggcg
    27481 ctggctttgc aaccaaagct ggtaaatatc gtcctgaacc tgactgccaa tacggaagtt
    27541 attaatcaaa atatgtgcgc catccggcag cgcttgctga tgcagtcgga tatggcagtt
    27601 ggcatccaca ttgacgattg ccagcgagtg atcgcacaaa ctcagcagct ggtgggttat
    27661 ctgcgaggca tcacgcggta agtcgattaa aatccactgg taacgcccgc tggcttttag
    27721 ttgctgtaag ccggagcaaa tatcgctcag ccgggtttgc cagtgctgtg gattttcttg
    27781 ttcttcaatg gataactgac caaaaggcag caaatcgagc tgcgaggtgt agcgcaaccc
    27841 agcgtcgcgc cagtcctagc catccagcat agctctggcc cagccctgac ggtgggtaaa
    27901 atcaacatta aatgacaggc gcaacaagtt gtccgggcag gcatcgacca ccaggacatt
    27961 ttctcccaac atttgtaatg accaggctaa tgcggcggtg atggttgttg tccccacgcc
    28021 tccccgcacc ccctgcaatc ccagtacggc cattcaccaa tcccctactt ttgttgcgca
    28081 aactctgcca gcaacggcca gcgttttaat gccgcggcca actgttcgcg ttgggaaata
    28141 tcggcataat caataccagg cagtgaaaat gcctgcttta acgccaaaat atcattctgg
    28201 aagatatagc ctatcgcggg atcaggcaga gtatctggtt cattgttatt cattttgctt
    28261 gatccctatg aaataaccgt accgttcatg agacaattct aaccgtgagc atttcgccgg
    28321 tttattgttt acggcgagac gggaatttgc tcactttttg actttccgtt acgcaacaga
    28381 aaaagttaaa aacgaaaaga ctaaaatcac acgtcttgtg acctgctaaa ttgattcaca
    28441 tgctaaatct gataagtttt aatttcaatg gtaggtttat ttcttagctt tcgctagtaa
    28501 actgataatc agacaaaatg gtgacatgag ggacattgtg gaccctgtat tctctatcgg
    28561 tatctcatca ttatgggatg agctgcgaca tatgccagca ggcggcgtct ggtggtttaa
    28621 cgtcgatcgc catgaagatg ctatcagtct ggcgaatcaa acaattgcat cccaggctga
    28681 aaccgcacac gtcgcggtca ttagcatgga cagcgatccg gcgaaaatct ttcaattaga
    28741 tgattctcaa gggccggaaa aaataaaatt attttcaatg ctaaatcatg aaaaaggtct
    28801 atactatttg gcccgtgatt tgcagtgttc tattgatccc cataattacc tttttattct
    28861 tgtttgcgca aataacgcat ggcaaaacat tcctgccgag cggctgcgct catggttgga
    28921 taaaatgaat aaatggagca ggttaaacca ttgttcgctt ttggtaatta atcccggaaa
    28981 taataacgat aaacaatttt cattgttgct tgaggaatac cgttcacttt ttggtcttgc
    29041 cagtttgcgt tttcagggcg accaacattt gctggatatt gccttctggt gcaacgaaaa
    29101 aggggtcagc gcccgtcagc agcttagcgt tcagcaacaa aatggtatct ggacattagt
    29161 tcaaagcgaa gaggcggaga tccaaccacg cagcgacgaa aaacgcattc tgagtaatgt
    29221 tgctgtactg gaaggtgcgc cgccgctatc ggaacactgg caactgttca acaataacga
    29281 agtcctgttc aatgaagccc gtaccgctca ggcggcgacg gtggtctttt ctttacagca
    29341 aaatgcgcaa atcgagccac tggcccgcag cattcatacc ctgcgtcgcc agcgcggtag
    29401 tgcgatgaaa atcctcgtgc gggaaaatac cgctagcctg cgcgccaccg atgaacgttt
    29461 gttattggcc tgcggtgcaa atatggttat tccgtggaat gcgccactct cccgttgtct
    29521 gacgatgatc gaaagcgtgc aagggcagaa gtttagtcgc tatgtgccgg aagatatcac
    29581 taccttgctg tcaatgaccc agccgctcaa actgcgtggt ttccagaagt gggatgtgtt
    29641 ctgtaatgcc gtcaacaaca tgatgaataa ccctctatta cctgcccacg gtaaaggcgt
    29701 tctggttgcc ctacgtccgg taccgggtat ccgcgttgaa caagccctga cgctgtgtcg
    29761 ccctaaccgt accggcgata tcatgaccat tggcggtaat cggctggtgc tgtttctctc
    29821 attctgtcgg attaacgatc tggataccgc gttgaatcat attttcccat tgcctactgg
    29881 cgacattttc tcaaaccgta tggtctggtt tgaagatgat caaatcagtg ccgagctggt
    29941 gcagatgcgc ctgcttgccc cagaacaatg gggcatgccg ctgcctttaa cgcaaagttc
    30001 taaaccggtc atcaatgccg agcacgatgg tcgccactgg cgacgaatac cagaacccat
    30061 gcgactgtta gatgatgctg tggagcgctc atcatgatga ccatcagcga tatcattgaa
    30121 attattgtcg tttgcgcact gatatttttc ccgctgggct atctggcgcg gcactctttg
    30181 cgacgcattc gcgacacctt acgtttgttc tttgctaaac ctcgttatgt taaaccggct
    30241 gggacgttac gccgcacgga aaaagccagg gcaaccaaaa aatgactcaa tttacgcaaa
    30301 ataccgccat gccttcttcc ctctggcaat actggcgcgg cctttccggc tggaacttct
    30361 attttctggt taagttcggc ctgttgtggg cgggatatct taacttccat ccgctcctca
    30421 atttggtgtt tgccgcgttt ctgctgatgc ccattccgcg ctacagcctg catcgcttgc
    30481 gccactggat tgccctgccg atcggctttg ctttgttctg gcatgacacc tggttgcctg
    30541 gcccggaaag cataatgagc cagggttcgc aggtggcggg gttcagtacc gattatttaa
    30601 tcgaccttgt cacacgcttt attaactggc agatgattgg ggccattttt gttttattag
    30661 tggcctggtt attcctgtca caatggattc gcattaccgt ttttgtggtt gccatactgc
    30721 tatggctgaa cgtacttacc ctggcgggac caagtttctc cttgtggcca gccggacaac
    30781 cgacgaccac tgtaacaacg acgggtggta acgcagcggc aaccgttgcg gcgacgggtg
    30841 gcgcaccggt agtgggtgat atgcccgcac aaactgcacc gccaacaacg gcgaacctta
    30901 acgcctggct gaataatttc tataacgcgg aggcgaaacg taaatcgacc ttcccgtctt
    30961 cgctgcccgc tgatgctcag ccatttgaac tactggtgat taacatctgt tcgctttcct
    31021 ggtcggatat agaagccgct gggttgatgt cgcatccact gtggtcgcat ttcgatattg
    31081 agttcaagaa ctttaactcc gccacctcct acagtggccc ggcggcgatc cgtttactgc
    31141 gcgccagctg cgggcagact tcgcacacta atctgtatca accggcaaat aacgactgct
    31201 atctgtttga taacctgtcg aaactgggct ttacccagca cctgatgatg ggacataacg
    31261 gccagttcgg cggttttttg aaagaagttc gcgaaaatgg cggcatgcag actgaattga
    31321 tggatcaaac aaatctgccg gttattttgc tgggctttga tggttcgccg gtttatgacg
    31381 ataccgccgt gcttaaccgc tggctggacg ttaccgaaaa agataaaaac agccgtagtg
    31441 ccacgttcta caacacgctt ccactgcatg acggcaacca ttatccgggc gtcagcaaaa
    31501 cagcggatta caaagcgcgg gcgcagaaat tctttgatga actggacgcc ttctttactg
    31561 aactggagaa atcgggtcgt aaagtgatgg tggtcgtggt gccggaacac ggcggcgcgc
    31621 tgaagggcga cagaatgcag gtatctggcc tacgtgatat ccctagcccg tctatcaccg
    31681 acgtccccgt tggggtgaaa ttcttcggca tgaaggcacc acatcagggg gcaccgattg
    31741 tcatcgacca accgagcagc ttcctggcta tctccgatct ggtggttcgc gttcttgatg
    31801 gcaagatttt caccgaagac aatgttgact ggaaaaaact caccagtggg ttgccacaaa
    31861 cagcaccggt ctcagagaac tcaaatgcag tagttattca ataccaggat aaaccgtacg
    31921 ttcgcctgaa cggcggcgac tgggtgcctt acccgcagta agcacgaaaa aggccgcaga
    31981 gtttcccccc tgcggtctgg tccgggcgca aattgccatt acggcagcct gacgcccgca
    32041 tgacacgtta cttccgcttg ttcagccagt tcacgatcat actggcaaga atgccagcaa
    32101 tgaccggagc cgctaaatca tgccagaagg ccatgcccag ctctgcgagc gtcatatagc
    32161 cgcctgtgtt gtaatgacaa cgtttcgcgg ctattcttga gtggtctagg ttcaagatta
    32221 gcccccgtgg tgttgtcagg tgcatacctg caacgtgcgg gggttttctc tctccagcaa
    32281 ccaatgccac cagggattaa gcccccgcaa cattgcgcct caccggaacc cttccggctt
    32341 gccgctaatt ttacgattgc tttttactgt cagcactcgg caatcttcct cattgcgtga
    32401 tgccttccag cttaatttct taatttgcaa tagttgcagt tcatatccgg gctgactcca
    32461 gactggtagt gaaatgcgca attttgtaga attgcgcctc ctcgcgacct ccccagtgaa
    32521 tctgttgcct cattttttac atactgcgta ttcgacttct ccacctgttg cgcaagagaa
    32581 actgggttta ttcatttttg cgaggccgac ttctttctgg acaggacttt atgcagcaca
    32641 acacactacc gaaacacgat cagaaattgc cgtttacacg ctacgacttc ggctgggttt
    32701 tattatgcat aggcatggcg attggtgccg gaaccgtgct gatgccagta caaattggct
    32761 tgaagggcat ttgggtattt attaccgcag cgatcattgc ttatcctgcc acctgggtag
    32821 tgcaggacat ttatttaaaa accctttctg aaagcgattc ctgtaatgac tacaccgata
    32881 ttatcagtca ttacctgggg aagaactggg gaattttcct cggggttatc tactttttga
    32941 tgattatcca cgggattttt atctactctc tctccgtggt tttcgacagc gcctcgtacc
    33001 tgaaaacctt cggtttaacc gatgccgatc tttcacaatc tctactttat aaagtcgcta
    33061 ttttcgccgt actggtagcg attgcgtctg gtggtgaacg attactgttt aagatttctg
    33121 ggccaatggt ggtggtcaaa gtagggatta ttgtcgtgtt cggttttacg atgatcccgc
    33181 actggaattt cgccaatata accgccttcc cgcaagcctc cgtctttttt cgcgatgtcc
    33241 tgcttaccat tccattttgc ttcttttctg cagtatttat tcaggtactt aacccaatga
    33301 atattgccta tcgtaaacgg gaagcggata aagtactggc aacccggctc gcgctgcgta
    33361 cccaccgaat tagttatatc acgctcatcg cggtgatact gttttttgcc ttttcgttta
    33421 ccttctcaat tagccacgaa gaagccgttt ctgcctttga acaaaatatc tcggcactgg
    33481 cactggccgc gcaggtgatc cctgggcata tcattcatat cacctctacg gtgctgaata
    33541 tctttgccgt actgaccgca ttctttggca tttatctcgg tttccacgag gccattaaag
    33601 gcattattct caatctgtta agccgaatta ttgataccaa gaaaattaac tcacgcgtgc
    33661 tgactctggc gatctgcgct tttatcgtca ttacgttgac gatttgggtt tcgtttcgtg
    33721 tatcggtgct ggtgttcttt cagttgggaa gcccgttata tggcattgtg tcgtgcctca
    33781 ttccgttttt cctgatctat aaagtcgcac aactggaaaa acttcgcgga tttaaaacct
    33841 ggctgattct gctttacggc attttgctat gcttgtcgcc actgttgaag ctgattgagt
    33901 aaaccggagc gcatggcccc ggttttgtga gttaacgctg cggattttca tcctgatcaa
    33961 cagcaaaaca agctaccagt tgaccgccgt agtcttttag ctgcggctgc aactgggtgc
    34021 aggggccgaa gcgccgacga cagcgggcgt taaacgcgca gcccggcggt ggattcagcg
    34081 ggcttggcag ttcaccggtg agtttgatgc gctcgcggcg atcgtccgga ttcaggcgcg
    34141 gcgtcgcgga aagtagcgcc tgagtgtacg gatggcgcgg gttattgaag atttggtctt
    34201 tcgttccctt ctccacgcag cggcccaggt acatcaccat cacttcatca gcaatgtgct
    34261 ccaccaccga caggtcgtgg gagatgaaga cataagaaag ccccagttcc tgctgcaaat
    34321 ccatcatcag gttgagcacc tgtgcacgca ccgagacgtc cagcgcggag accggttcat
    34381 cggcaatcac cacgtccggg tcgagcatca gacctcgggc aatggcgata cgctgacgct
    34441 gaccgccgga gaacatatgc ggatagcggt catagtgctc ggttttcagg ccgactttcg
    34501 ccatcatcga cagggctttt tcccgacgct gttctttgct taagctggtg ttgatcagca
    34561 gcggctcttc aagaatttgc ccgatttttt tacgcggatt cagcgaaccg taagggttct
    34621 ggaagacaat ctggattttc tgccgacgca gcttctgcgc ctgcggatcg tgcttaagca
    34681 gatcctgccc ctggtaatac agctcgccgc cggtgggcgt ttcaatcatc gtcagcaacc
    34741 gaccgagggt cgatttaccg cagccagatt cacctactac cgccagcgtt ttgccacgtt
    34801 caaggttaaa cgaaacgccg tccagcgcct tcaccagacg ttccggagcg aacatacctt
    34861 tcttcaccgg ataatgtttt ttcaggtcga tagcctgcaa cagcggttgt tgcgaggtgg
    34921 cctcttgcgt actcatagtg tcggcctccc ggcatcatca agtgggtaat ggcatttgga
    34981 ctgacgcccg tcagcgagca tattcagcgc cggttcttca gcgcgacatc tgtcagtggc
    35041 ataggggcag cgcgggttaa gcaagcagcc gttcgggcgg tcgtacttgc cgggaacaac
    35101 gccaggcaac gatgccagac gttctttgtc ctgagcaaat tccggcagcg cacgcagcaa
    35161 tgcctgagta tacgggtgac gcggcgcatg gaagatggcg tgcgcatcgc ccgtttccac
    35221 cacctgaccg gcatacatca cgatgatttt atgtgccgct tccgccacca gcgccaggtc
    35281 atgggtaatt aacaccagcg ccatgttctc tttctgctgt agctccagca gtagttcaat
    35341 gatttgcgcc tgaatggtca cgtccagcgc agtggttggt tcatcggcaa tcagcagttt
    35401 tggccgacag gcaatggcca tcgcaatcat cacgcgctgg ctcatgccgc cggaaagctg
    35461 atgcgggtaa acatccagac gcgaagccgg atcgggaata ccgacctgat tcaacaagtc
    35521 aatcgcccgc tgacgacgag tgctcttatt accgccctga tgcaccttaa tcgcttccat
    35581 aatctggaaa cccacggtgt agcacgggtt aaggctggtc atcgggtcct ggaagatcat
    35641 cgccacttcg gcacccacca ggttgcggcg ctctttttcc gagatacgct gcaaatcctg
    35701 accgttaaac tccagctttt ccgccatcac gcggcccgga taatcaatca gccccataat
    35761 cgccagcgaa ctgaccgact taccggagcc agactcaccc acaatcccga ctacttcgcc
    35821 ctgttttacg ctgtagctaa tgcggtctac ggcgcggaac ggtgcgcttt cgtcgccgaa
    35881 atgcaccgat aatttatcta catttaataa cgccatctcg aacctcttac tgcttcagtt
    35941 tgggatcgag cgcgtcacgc agaccgtcac ccatcaggtt aaatgccagc accgtcagca
    36001 ggatcgccag acccgggaag gtcacgaccc accaggcgct ttgcgcgaac tgcaacacgt
    36061 cggagagcat ggtgccccac tccggcgttg gcggctgcgc ccccatgccg aggaaaccaa
    36121 gagcagccat atcgagaatg gcgttagaga aaccgagcga cgcctgaaca atcagcggtg
    36181 caaggcagtt cgggaagatg ttaataaaca tctggcgcat cgccccggct cctgccacgc
    36241 gagaagcggt gacgtaatcg cgattcacct ccaccagcac ggcggcgcgg gttaagcgca
    36301 catagtgcgg caaggcaaca aaggtcagcg ccagcgcggc gttaccaatc gacgggccga
    36361 aaatcgccac cagcaccagt gccaacagca gacttggcag cgccagcatg atatcgacca
    36421 cgcgcataat gatgttatcg accaggccgc cgaagtaacc ggcgatcagg ccgagaataa
    36481 cgcccataat cagcgataac accaccacca gacagccaac cagcagcgac aagcgcgcgc
    36541 cgtacatcag ccgcgacagc acatcacggc ctacatcatc ggtacccagc aagtgcgcca
    36601 tgctgccgcc ttcctgccag gctggcgggg cgagcagtgc atcgcggaac tgttccgccg
    36661 ggttataggg agcaatccag ttggcaaaga tcgcgataaa cagcacgatg acgacgtaaa
    36721 ccagcccgac gaccgcgcct ttgttgcgtt taaaatagtg ccagaactcc tgtaaagggg
    36781 tcatcggtac cggcgcgcta atcactttat tttcagtaac ctgtgacatg atgacccctt
    36841 acttcttatg acgaatacgc gggttcacca cgccatacag cagatcgacc agcaggttga
    36901 cgaggataat catcgtcgcc accagcaata cgccgccctg caccaccgga taatcgcgcc
    36961 gttgcagtgc gtcaatcaac cagcgcccca gaccgggcca cgagaagatg gtttcggtca
    37021 gaatcgcccc cgccagcaat gttcccacct ggaggccgat aacggtcact accggcagca
    37081 tcgcgttacg cagcgcatgg acgataatca cccgcatgcg ggtcaaccct ttggcgcgcg
    37141 cggtgcggat gtaatcttca cccagcactt ccagcatcga ggagcgtgtc atacgcacaa
    37201 tgaccgccag cggaatagta cccagcacaa tggcgggcaa gatcatatgg gcgacggcat
    37261 cgataaagtt accgtcttca ccccagatgg cggtgtcgat cagcataaaa ccggttaacg
    37321 gattggaatc atcgaggaac accatatcgc tcacgcgacc ggagacgggt gtcaggttcc
    37381 agtgcaccga aaccagcatg atcagcatca tgccccacca gaagataggc attgaataac
    37441 ctgtcagcgc caggccaacc gctgtgtgat cgaaaatgga accgcgttta accgcggcca
    37501 gcacaccaac cggaatgccg accgcggtcg caaaaatcat cgcgcagacg ccaagttcca
    37561 gcgtggcctg aaagcgcggc acgaactctt cccataccgg aattcggctt ttcattgaaa
    37621 tgcctagatc gccatgcata acgccccaaa tgtaatggag atactgctgc cacatcggtt
    37681 tatctaagcc gagttcagcc agcaactgcg cgtgacgctc tggggagatc ccacgttcgc
    37741 ccgccatgat catcacagga tcgcccggga tcatgtggac aaaggcaaat gtgagaaggg
    37801 taataccgat aaacgtgggg atgacgagtc ccaaacgtcg gagaataaac tgcaacataa
    37861 cccggattct ctgtagtgac ccgcagcctg gaactgcgtg tctgtattgc tcacaaatca
    37921 attcccgccc aggtcgggca gggaatgaag tattgctgcc ggatggcgct gcacgcagag
    37981 gcacagcacc accgggtatg gcttttaatt attcgataga gacgttttcg aagtgatgtt
    38041 tgcctaatgg atcaaccaca tagcctttaa cttctttgcg taccggttca aacacggtgg
    38101 agtgagcgat gatcagtgcc ggagcctgat cgtgcatcac cacctgcgct tgcttgtaca
    38161 gttcaacgcg tttgttatgg tcgtcggtag cacgcgccgg ttgaatcaga tcttcaaacg
    38221 gtttgtagca ccattttgag tagttggagc cttgttcaga ggcggcgcag ctgaacagag
    38281 tggcgaagaa gttatccgga tccccgttat cgccagtcca gcccatcatt accgtctggt
    38341 gctcgccatc tttcgcacgc ttgaggtact caccccattc gtaggtgaca attttggcct
    38401 gcacgccgac tttcgcccag tctgcctgaa tcatctccgc catgcggcga gcgttcgggt
    38461 tatacggacg ttgtaccggc atcgcccaca ggtcgatgga gaaacctttt tccagacccg
    38521 cttctttcag caaggctttc gctttttcag gatcgtaggt gtagtcctga acgtcgtcgt
    38581 tatagcccca catggttggc gggatcaggt ttttcgctga tacacccgcg ccctgataaa
    38641 ccgctttgat gatcgcgtct ttgttcaccg cgtaggtcag agcctggcga actttcacgt
    38701 catcgagtgg ttttttctgc acgttatacg agagataacc gacgttcagc cccggcattt
    38761 ccatcaggtt gatagattta tcctgcttca tgcgggcgat atctgccggg ttcgggtacg
    38821 gcatcacctg gcattcattt ttctgcaatt tagcgtaacg cacggaagcg tcaggggtaa
    38881 tagagaaaac cagcgtatcg atctgcggtt tggtgcccca gtagccatca aacgctttgt
    38941 agcggatacg ggaatctttt tgatactgct gtaactggaa cggaccggtg ccgattgggt
    39001 tgaggtccag tttttccggt gtaccggctt tcatcatcgc atcagcatat tcttttgaca
    39061 gaatagaggc gaagtccatt gccaggtcag cgaggaacgg cgcttccggg cgagtcagca
    39121 caaactgaac ggtgttgtcg tccacctttt tcacttcgct gatcagctcc ggcaagccca
    39181 tgccttcgaa gtattcgtag ctgccgccag aaactttatg gtacgggttt tgcgcgtttt
    39241 tctgacgatc gaacgagaac accacgtcat cggcattcat ttcgcgcgtc ggtttgaatt
    39301 ctttattgtc gtgccacttc acacctttac gcagatggaa ggtataggtt ttaccgtctt
    39361 cgctgacttc ccacttttca gcgaggcccg gaatcacttc ggtggtgccg attttaaatt
    39421 caactaggcg gttataaagc ggtacggaag aggcgtcata ggtggtaccg gaggtaaaca
    39481 gctgcgggtt aaacccttcc ggagatcctt ctgagcaata aaccagagtt ttagcctgaa
    39541 cacttgctgc gacggtcata gccaccaggc tgagaccaag cttcagcatc cctgactttt
    39601 tcaaggaaat acgcattatt ctgctccaat tgtgatgttt gttgttttaa ccctttgcag
    39661 tgggttgtcg ctgcctgacc ttttttgttt tttgcccggt cgggtcaacg ttatgaggtg
    39721 gggatgccgt actaattaac atcagtgtaa cagtgcggat cctccataaa atgcccctcg
    39781 tgacctacaa tctgtcaaca gaatgtgaaa acgtcaatac agccaaccgg gatttacacc
    39841 aacggtgaga atccacaaac aaagattaaa aaaacttcaa acagctattt gcagcagaga
    39901 aatttgtgct actccaacat gaccagaaca atcagcttaa tatttagcaa catttggtga
    39961 ttaagtttta tgcatttagt gaaaaaaatc tg
//
LOCUS       DBFIFS010000020        39795 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.020, whole genome shotgun sequence.
ACCESSION   DBFIFS010000020 DBFIFS010000000
VERSION     DBFIFS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.020"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..152)
                     /locus_tag="N7A07_000961"
     CDS             complement(<1..152)
                     /locus_tag="N7A07_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2475679.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGL"
     gene            complement(503..730)
                     /gene="ygdI"
                     /locus_tag="N7A07_000962"
     CDS             complement(503..730)
                     /gene="ygdI"
                     /locus_tag="N7A07_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU2475680.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            922..2127
                     /gene="csdA"
                     /locus_tag="N7A07_000963"
     CDS             922..2127
                     /gene="csdA"
                     /locus_tag="N7A07_000963"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU2475681.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            2127..2570
                     /gene="csdE"
                     /locus_tag="N7A07_000964"
     CDS             2127..2570
                     /gene="csdE"
                     /locus_tag="N7A07_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU2475682.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2621..3427)
                     /gene="tcdA"
                     /locus_tag="N7A07_000965"
     CDS             complement(2621..3427)
                     /gene="tcdA"
                     /locus_tag="N7A07_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU2475683.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3666..4763)
                     /gene="mltA"
                     /locus_tag="N7A07_000966"
     CDS             complement(3666..4763)
                     /gene="mltA"
                     /locus_tag="N7A07_000966"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU2475684.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4972..5048
                     /locus_tag="N7A07_000967"
     tRNA            4972..5048
                     /locus_tag="N7A07_000967"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5006..5008,aa:Met,seq:cat)
     gene            5082..5158
                     /locus_tag="N7A07_000968"
     tRNA            5082..5158
                     /locus_tag="N7A07_000968"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5116..5118,aa:Met,seq:cat)
     gene            complement(5232..6485)
                     /gene="amiC"
                     /locus_tag="N7A07_000969"
     CDS             complement(5232..6485)
                     /gene="amiC"
                     /locus_tag="N7A07_000969"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU2475685.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            6717..8048
                     /gene="argA"
                     /locus_tag="N7A07_000970"
     CDS             6717..8048
                     /gene="argA"
                     /locus_tag="N7A07_000970"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU2475686.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKSCRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(8110..9936)
                     /gene="recD"
                     /locus_tag="N7A07_000971"
     CDS             complement(8110..9936)
                     /gene="recD"
                     /locus_tag="N7A07_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU2475687.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(9936..13478)
                     /gene="recB"
                     /locus_tag="N7A07_000972"
     CDS             complement(9936..13478)
                     /gene="recB"
                     /locus_tag="N7A07_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU2475688.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(13471..16359)
                     /gene="ptrA"
                     /locus_tag="N7A07_000973"
     CDS             complement(13471..16359)
                     /gene="ptrA"
                     /locus_tag="N7A07_000973"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU2475689.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16535..19903)
                     /gene="recC"
                     /locus_tag="N7A07_000974"
     CDS             complement(16535..19903)
                     /gene="recC"
                     /locus_tag="N7A07_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU2475690.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19916..20239)
                     /gene="ppdC"
                     /locus_tag="N7A07_000975"
     CDS             complement(19916..20239)
                     /gene="ppdC"
                     /locus_tag="N7A07_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU2475691.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(20224..20631)
                     /gene="ygdB"
                     /locus_tag="N7A07_000976"
     CDS             complement(20224..20631)
                     /gene="ygdB"
                     /locus_tag="N7A07_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU2475692.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(20628..21191)
                     /gene="ppdB"
                     /locus_tag="N7A07_000977"
     CDS             complement(20628..21191)
                     /gene="ppdB"
                     /locus_tag="N7A07_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU2475693.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSLVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(21182..21652)
                     /gene="ppdA"
                     /locus_tag="N7A07_000978"
     CDS             complement(21182..21652)
                     /gene="ppdA"
                     /locus_tag="N7A07_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU2475694.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(21836..22630)
                     /gene="thyA"
                     /locus_tag="N7A07_000979"
     CDS             complement(21836..22630)
                     /gene="thyA"
                     /locus_tag="N7A07_000979"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU2475695.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22637..23512)
                     /gene="lgt"
                     /locus_tag="N7A07_000980"
     CDS             complement(22637..23512)
                     /gene="lgt"
                     /locus_tag="N7A07_000980"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU2475696.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(23663..25909)
                     /gene="ptsP"
                     /locus_tag="N7A07_000981"
     CDS             complement(23663..25909)
                     /gene="ptsP"
                     /locus_tag="N7A07_000981"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2475697.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25922..26452)
                     /gene="rppH"
                     /locus_tag="N7A07_000982"
     CDS             complement(25922..26452)
                     /gene="rppH"
                     /locus_tag="N7A07_000982"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU2475698.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(26805..26951)
                     /gene="ygdT"
                     /locus_tag="N7A07_000983"
     CDS             complement(26805..26951)
                     /gene="ygdT"
                     /locus_tag="N7A07_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU2475699.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            27137..27826
                     /gene="mutH"
                     /locus_tag="N7A07_000984"
     CDS             27137..27826
                     /gene="mutH"
                     /locus_tag="N7A07_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU2475700.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            27895..28608
                     /gene="ygdQ"
                     /locus_tag="N7A07_000985"
     CDS             27895..28608
                     /gene="ygdQ"
                     /locus_tag="N7A07_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU2475701.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            28746..28964
                     /gene="ygdR"
                     /locus_tag="N7A07_000986"
     CDS             28746..28964
                     /gene="ygdR"
                     /locus_tag="N7A07_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU2475702.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            29072..30112
                     /gene="tas"
                     /locus_tag="N7A07_000987"
     CDS             29072..30112
                     /gene="tas"
                     /locus_tag="N7A07_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU2475703.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(30144..31337)
                     /gene="lplT"
                     /locus_tag="N7A07_000988"
     CDS             complement(30144..31337)
                     /gene="lplT"
                     /locus_tag="N7A07_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU2475704.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(31330..33489)
                     /gene="aas"
                     /locus_tag="N7A07_000989"
     CDS             complement(31330..33489)
                     /gene="aas"
                     /locus_tag="N7A07_000989"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU2475705.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKHRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            34075..35106
                     /gene="galR"
                     /locus_tag="N7A07_000990"
     CDS             34075..35106
                     /gene="galR"
                     /locus_tag="N7A07_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU2475706.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(35113..36375)
                     /gene="lysA"
                     /locus_tag="N7A07_000991"
     CDS             complement(35113..36375)
                     /gene="lysA"
                     /locus_tag="N7A07_000991"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU2475707.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            36497..37432
                     /gene="lysR"
                     /locus_tag="N7A07_000992"
     CDS             36497..37432
                     /gene="lysR"
                     /locus_tag="N7A07_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU2475708.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(37419..38111)
                     /gene="ygeA"
                     /locus_tag="N7A07_000993"
     CDS             complement(37419..38111)
                     /gene="ygeA"
                     /locus_tag="N7A07_000993"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU2475709.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(38240..39658)
                     /gene="araE"
                     /locus_tag="N7A07_000994"
     CDS             complement(38240..39658)
                     /gene="araE"
                     /locus_tag="N7A07_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU2475710.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLS
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         9735 a        10893 c         9847 g         9320 t
ORIGIN      
        1 tttagcccca aaaaatcctc aagagacttg atttgatgac ttactgcggc ctgggtcaca
       61 aaaagctctt ctgctgcgcg agtgaaactt aaatggcgtg ctgcggcatc aaaaactcgt
      121 aaggcattta gcggtggtaa tcgtttagac atggctttta aactttgatg ttaaatgaat
      181 ttaacaatta gatcacactg tgtaacctat tagttttttt aatctgagcc attataaatt
      241 gtccgttgag cttctaccag caaataccta tagtggcggc acttcctgag ccggaacgaa
      301 aagttttatc ggaatgcgtg ttctggtgaa cttttggctt acggttgtga tgttgtgttg
      361 ttgtgtttgc aattggtctg cgattcagac catggtagca aagctacctt ttttcacttc
      421 ctgtacattt accctgtctg tccatagtga ttaatgtagc accgcctaat tgcggtgctt
      481 ttttttacct tgcgatcgcg aattactgat ccagttcgac catctctttc acgtcggtac
      541 ggttgatctg ctgtttgttg ccattagcgt ctttatacga aatcataccg gtatcgttat
      601 cagtctgtgg tttgccgtca gagacgatgg tacgtccgtc attggtgtgc atcacatagt
      661 tcgaaccgga acaggcgctc agggcaaaag tcagcataca ggcagaaata attgcggcag
      721 tctttttcat catcttctcc ttaaagcgat taataaatct taatagtatt gattaacagg
      781 ctaaaattaa cgcctaacac tattcagcat atgttactga cgcggcttcg ccaggatatc
      841 cagataattc tgatggttag cactctcctt gtatcaaagt gaattttgcg tcacgatcgg
      901 tgcatcaagc cgaggagtac catgaacgtt tttaatcccg cgcagtttcg cgcccagttt
      961 cccgctctac aggatgcggg cgtctatctc gacagcgccg cgaccgcgct taaacctgaa
     1021 gccgtggttg aagccacccg acagttttac agtctgagcg ccggaaacgt ccatcgcagc
     1081 cagtttgccg aagcccaacg cctgaccgcg cgttatgaag ctgcacgaga gaaagtggcg
     1141 caattactga atgcaccgga tgataaaact atcgtctgga cgcgcggcac cactgaatcc
     1201 atcaacatgg tggcacaatg ctatgcgcgt ccgcgtctgc aaccgggcga tgagattatt
     1261 gtcagcgtgg cagaacacca cgccaacctc gtcccctggc tgatggtcgc ccaacaaact
     1321 ggagccaaag tggtcaaatt gccgcttaat gcgcagcgac tgccagatgt cgatttgctg
     1381 ccggaactga ttactccccg tagtcggatt ctggcgttgg gtcagatgtc gaacgtaact
     1441 ggcggttgcc cggatctggc gcgagcgatt acctttgctc attcagccgg gatagtggtg
     1501 atagttgatg gtgctcaggg ggcggtgcat ttccccgcgg atgttcagca actggatatc
     1561 gatttctatg ctttctctgg tcacaaactg tatggcccga cgggtatcgg cgtgctgtat
     1621 ggcaaatcag aactgctgga agcgatgtcg ccctggctgg gcggcggcaa aatggttcac
     1681 gaagtgagtt ttgacggctt cacgactcaa tctgcgccgt ggaaactgga agcaggaacg
     1741 ccaaatgtgg ctggtgtcat aggattaagc gcggcgctgg aatggctggc agattacgat
     1801 atcaaccagg ccgaaaactg gagccgtagc ttagcaacgc tggcggaaga tgcgctggcg
     1861 aaacgtccag gctttcgttc attccgctgc caggattcca gcctgctggc ctttgatttt
     1921 gctggcgttc atcacagcga tatggtgacg ctgctggcgg agtacggtat tgccttgcgg
     1981 gccgggcaac attgcgctca gccgctactg gcagaattag gcgtgaccgg cacactgcgc
     2041 gcctcttttg cgccatataa tacaaagagt gatgtggatg cgctggtgaa tgccgttgac
     2101 cgcgcgctgg aattattggt ggattaatga cgaacccgca attcgccgga catccgttcg
     2161 gcacaaccgt aaccgcagaa acgttacgca ataccttcgc accgttgacg caatgggaag
     2221 ataaatatcg ccagttgatc atgctgggga aacagcttcc ggctttgcca gacgagttaa
     2281 aagcgcaggc taaagagatt gccggatgcg aaaaccgcgt ctggctggga tataccgtgg
     2341 ctgaaaacgg taaaatgcac ttctttggcg acagcgaagg acgtatagtg cgcggcctcc
     2401 tggcggtgct gttgactgcc gttgagggga aaaccgccgc cgaattgcag gcacagtcgc
     2461 cgctggcatt gtttgatgag ctgggattgc gtgcgcaact tagcgcctca cgcagccagg
     2521 ggttaaatgc gttaagcgag gcgattatcg ctgcgacgaa gcaggtttaa gcacagaacc
     2581 gggtcggata agacgtttgc gtcgcatccg acctgattgt ttacccctga cgcgccgctt
     2641 tcgccatcat cttcttcagc gcatgagaaa ccgcaacaaa accaaaggtg gcggtcacca
     2701 tcgttgccgc gccaaatcca gatgcacaat ccatccgctt tggtccttcc gccgtggctt
     2761 tcatcgcaca caccgtaccg tctgactgcg gatacaccaa tgcttcggta gagaacacgc
     2821 aatccacacc gagcttacct ttactgtttt tcactacgcc aaaatcgctt ttcaggcgct
     2881 cacgcaactt cgccgccagc gggtcctgaa tcgttttcgc cagatcggta acctgaatct
     2941 gcgttgggtc aatctgccca cccgcgccac cggtcgtgac cagcgggatt ttattgcgcc
     3001 gacaataagc aatcagcgcc gctttgggcc gtacactatc aatggcatca atcacgtacg
     3061 aataacccac gctcatatgc tgcgctacgt tatccggcgt cacgaaatca tccaccaccg
     3121 ttacacagca ctccgagtta atctggcgaa tacgttccgc catgacttcc gcttttgcca
     3181 gcccaacgtt atcgcgcagg gcatgaattt gccgattggt attggtgacg cacacatcat
     3241 ccatatcgat aagcgtgatt gcgccaatcc ccgtgcgcgc cagcgcttcc gccgcccagg
     3301 aaccgacacc gccaataccg accacacaaa tatgcgcgtc agcaaacagt tgcaacgctt
     3361 tttcaccata cagacgcgct gtgccaccaa aacgctgacg ccatgcatca ctaattacca
     3421 cagacatagc acctcagaat taaaaagggc gaggttatcc ctcaccctaa acgcataaat
     3481 gcctgatgcg ctacgcttat caggcctaca taactcctgc aatattttgg atttgcatga
     3541 tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaacgcgc acttagtcaa
     3601 taatcaggat gtgaggttac ccctcaccct gtcatatccg taaaaacggc atacagaata
     3661 tcacatcagc cgctaaagac gttacctgcg cccggggcgg ttttcagcac ccagacacgt
     3721 ccatagtggt tgtaccaacc tgcgcggtgt ccggcttccg gcccgatccc ttgataaata
     3781 tcgaagtgtt ggcctttgat tgcgccaccg acatccaacg ccaccatcag acgcaattcg
     3841 tactgaccgt taaatttgcc gttattatcc agcagcggca cttctgccag caaggttgta
     3901 cctggtggga taatggaacg atcagaggca acagaagcac ggccaaccag cggcaccgca
     3961 cttgcccctt tcaccggtgc aaaagattgc ggtttaaaga aaacgaaaga cgggttctgt
     4021 tccagcagct cgcgaacctc ggcttcactg tgtgtttcgc cccaatgacg aatcgcctgc
     4081 atcgacatat cttctttttt cacttcgcca cggtcgatca gcaccttacc aatgctgcga
     4141 taggcatgac cgtttttccc tgcatagctg aaaaagttaa gcggactgcc atcaccaaag
     4201 tcgatatacc cactaccctg cacatccata atgaaattat ccatcagaga gttgctgtaa
     4261 gcgaggatgt acttatcgct caatgcaccc gcgtagatct ccgcacgaga cggcagacga
     4321 ccacgttttg gcggcatacg gtaaatagga tactggaact cgccctggcg ggtatggcgc
     4381 gcctgaatta ccggcgtgta atagcctgta aactgcacgt taccatagtt gtcggcacct
     4441 tccatctgcc aggcatcaat gccgaactgg cgcatattgc gggtatcacc gcctgcgcgc
     4501 agccactctt gcaccgcgtt ataaacatta ctctggttgc catacagacg cggtgacgaa
     4561 ttacggatat ggttaatttg ctcggcaaaa tcaccggcgt taatcggcgc gccaacggca
     4621 tctggctggt tcaccagaga gaaaggctgg gtaaatttcc cgtctttata ttgctgtccg
     4681 cgatcggttg gtttggaaga gcaggcggca agcattgcca caaccgtgcc cataagaagg
     4741 tactttaccc aacgtccttt cattgttctc ttcttcaggt taaaaaataa ggcgcaacga
     4801 agataacaaa ccaccgcgtt taatgaaatg agcagcctct ccctgacgca aattttgcac
     4861 aaaaaatagg ctttagtgat ttgtttttgt tcaaaatcat gccagatccg tgatcggggt
     4921 aaaaaaaagg ttgcatgaaa acgcgagcgg agtatagtgc gcatccacgg acgcggggtg
     4981 gagcagcctg gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa atccggcccc
     5041 cgcaaccaat taaaatttga tgaagtaaag cagtacggtg acgcggggtg gagcagcctg
     5101 gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat
     5161 tattgaacac cctaacgggt gtttttttgt ttctggtctc ctataaaaaa gcgccattca
     5221 gcgccttttt atcatcccct tctcgccagg gtcgccccat cggcaaaata cgctttaatt
     5281 cccgcaagaa tagactccgc aacttcctgc tggaaggtcg ccgttttcag tttacgctct
     5341 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
     5401 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat tttaccgagc
     5461 ttattcagca ccgctttacc aaacttaagg ctgtcagcaa tggtcagcga ctgcaccata
     5521 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcg
     5581 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag
     5641 agcgcaaaca ccgaggaacc gctcggctgg cgactggtaa aggcgtcggc atggatagag
     5701 acaaacaggt cagcacgctg tttctgagct tttgctacgc gcacttgcaa cggaatgaag
     5761 atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc
     5821 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
     5881 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
     5941 gctttacctg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
     6001 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
     6061 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
     6121 tttacgtttt gctttaattc aaaaaccatc cgtacggttt gcgggtcaaa ttgcccgacg
     6181 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     6241 gagttcaggt ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac
     6301 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     6361 cagacgcgca ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat
     6421 agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     6481 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     6541 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     6601 atattcacgc cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     6661 cgaaaaaaca gaataaaaat acaataaatt cgaataataa tgcaaagagg tgtgccgtgg
     6721 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     6781 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     6841 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     6901 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     6961 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     7021 gaacattgca actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctac
     7081 agggcgcgca tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg
     7141 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     7201 atcgtcaact ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     7261 gcgagagctt taatctgacc tcggaagaga ttgccactca actggccatc aaactgaaag
     7321 ctgagaagat gattggtttt tgctcttccc agggcgtcac taatgacgac ggtgatattg
     7381 tctccgaact tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg
     7441 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaatcctgc cgcagcggcg
     7501 tgcgtcgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     7561 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     7621 caatcaacga tatcggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     7681 tggtacgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     7741 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     7801 aaatggcctg tgtggcagtt cacccggatt accgcagctc atcacggggc gaggttctgc
     7861 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     7921 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttattgc
     7981 ccgaaagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     8041 tagggtaagc gatgaaaatc gtcggatgcg acatgcgtaa cactcgtacg tcgcatccgg
     8101 caattacgtt tattcccgtg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     8161 agtggcgatt gccgcactta atatgcgctc atcggcatac agcgacagac ggcgacgcgc
     8221 gcgggtcacc gcggtataaa ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     8281 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     8341 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     8401 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     8461 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     8521 cggattacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     8581 agccacgcca aatggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg
     8641 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc
     8701 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagcc gtttttcaat
     8761 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     8821 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     8881 gagactgtcg cgcaaagatg ccgcttctgt gccagttcct gccggaacgt gagttcccgt
     8941 caggcggctt agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat
     9001 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     9061 cactcgcgca tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     9121 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     9181 acgctggcta cccggctgcg cacccagcaa tcggtgcaaa gtactggcat cttccggaat
     9241 acgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     9301 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     9361 ggccatttga attaacgctg ccagcaactt cgctacggtg gtcgttttac cggtgccagg
     9421 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gctgccgcca ctttttgcca
     9481 gttaatttca tcaccagtcg gaaagagttt atccagggtt tgcgccaaca aggcttcatc
     9541 cacctcgatg gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     9601 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctccccg
     9661 gctgaccgct tgtgaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     9721 gacacaggtc gccaacagcg gatgcgactc ctcattattt tccagtcgtg aaagcggcaa
     9781 acaaacgtgc ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     9841 atgttcatct cccgccacgg tcagggcaaa ctgcacgtcc agcgggcgta gctgtttgtg
     9901 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag agtcataccg
     9961 gcaaacatct catccatcag gtcaatcaac ccggcgttgg ggcgggttgt gtaaatccct
    10021 tgttgcggat gttctttatc aacaccacgc aggaacagat aaataacgcc gccaaagtgg
    10081 cgctcatagt cgtaatcagc aatgcgatgg cgcagataac ggtgcagcgc cagggtataa
    10141 agctgatatt gcagatcata gcggtgtgcc tgcattgccg ctgccatagc ctgttgggtg
    10201 taagccgaac tatcttcacc caaccagttg gatttatagt cgagcaggta ataacgccct
    10261 tcgtggcgga acaccaggtc gataaagcct tttaacatgc cacgtgcctg catgaactcc
    10321 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
    10381 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
    10441 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
    10501 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
    10561 acccagttcg ggtcaaccgg ctgggtaaaa ttgaggtctt caaacaaact gtgcaagaat
    10621 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
    10681 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
    10741 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
    10801 agcgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
    10861 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
    10921 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagggc actttggtgg
    10981 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
    11041 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
    11101 tcgacctccg caagatcgac gctttctggc gcagcgttaa gatccagaac agcctcaaac
    11161 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
    11221 cagaccagcg gatattccag cccttttgat ttatggatcg tgacaatctg taccagatgt
    11281 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
    11341 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc
    11401 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
    11461 ttttctgcaa tgttacgcgc cgacatcagc gcccgcatca tcggcataac gccacgtttg
    11521 cgccagattt gccgataacc atcgaactct tcgaccaccg cgtcccacgc atgttcgtca
    11581 ttattcagcg tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcacta
    11641 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
    11701 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
    11761 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg
    11821 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
    11881 ccggcttgta gccagtcgcg aatttgcgca gcacttacct gcgccatggt actttgatag
    11941 tcgccaacgc cgcaactttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
    12001 cctttaaaga caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
    12061 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
    12121 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
    12181 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
    12241 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
    12301 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
    12361 gccgctgcca acacctcacc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
    12421 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
    12481 gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
    12541 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
    12601 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttct ctcttcttct
    12661 gcccaggcgc tgatcttgtc gatccattta gcctgattgc tacggttaaa cttgcgtcga
    12721 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
    12781 tttaccgtat caatacgcgc cacaatttgc gcgtgacggg aagccagcgt ttcatcatcg
    12841 ggcggcggtg ctttgataac cggcgcttca ccttgcagat aacgattaat atcgcgcagc
    12901 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
    12961 tagcagtggc gacgccagaa atcggcgcag gcctggtaac gtagcagaga ctcatcttca
    13021 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
    13081 caaaaaccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
    13141 cacttcgcgg cttgcgcttt atcgtcgatc tcttccagca gacgttcgta cagtggattg
    13201 tcggtggttt cacgcagaca ggcgatgcgc aactcatgga tattgctacg gatacgacca
    13261 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc
    13321 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
    13381 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgaaagggc
    13441 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
    13501 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
    13561 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
    13621 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
    13681 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcaa
    13741 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
    13801 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
    13861 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
    13921 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
    13981 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
    14041 tgagcttgtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
    14101 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
    14161 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
    14221 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
    14281 ggcgtcacga taagccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
    14341 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
    14401 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
    14461 ctgatcttcc gtagcggtat agctaaagta tccctcgagc aatgcctgga acagctgcgg
    14521 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
    14581 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
    14641 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
    14701 aatcaggctg acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac
    14761 gcgcagattc gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat
    14821 cagcgagaaa tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc
    14881 agctttttgc tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc
    14941 atcgacaaaa taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc
    15001 attctgcggc gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc
    15061 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
    15121 ttcgacgtaa tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt
    15181 cgccagttca tcgaagtatt gtttatcgat ccctttttca cgtaacaaat tgaggtagct
    15241 aaaaatagcc gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga
    15301 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    15361 aactaacccc tgcttttgca gccagtcaga cagtgtacct gggctgcgat taccaatcag
    15421 ataggtaatc aattcatccg ttttactgcg gaacttcgct gagttgttat cgatgcgaaa
    15481 ctcaacgcgc agaactttac gcggcagcgc tgggacgtaa tgaatgataa tgcccttttg
    15541 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    15601 accaaaagtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    15661 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    15721 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    15781 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgtgtacg
    15841 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    15901 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    15961 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    16021 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    16081 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    16141 gggcaccacc agcgccgata gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    16201 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    16261 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac
    16321 taacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    16381 ttattttctg gccagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    16441 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    16501 taagcggata gattgcgcaa tttttataca gcgctcatga ctgattaaag cgaaacagcg
    16561 gtaacaggaa acgttgcgac tgttcaacga ttgcctccat tgtctctggt gttaattgcc
    16621 gccagagcct ttgataccag atatcatcac cttcgccact caccatcatg ttgccttcgt
    16681 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    16741 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    16801 atggcgcgga catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    16861 gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt
    16921 caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    16981 ccacacttaa taaagaggga cgccagcgca acaagccatc cggctgcacc tgcggcaacc
    17041 agccagttat ctgcacaccg ttgcaagcga gatcaatttc catactctgc cccggctggc
    17101 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    17161 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    17221 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    17281 gtccttccag aataaatggc tcggtgtcgg ggatttcgct gtcttcagta cggaagttca
    17341 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    17401 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    17461 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    17521 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    17581 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    17641 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    17701 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    17761 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    17821 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    17881 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggcccggcga
    17941 gaaaacgctg gctgatacgt tcttgatcca ggcgctgtgc cagttcatca cgcaatagtg
    18001 acagcggcac cgcatcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    18061 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag agggcgttga
    18121 gcatatcgcg acaaaccggc aaccactctt ccagcggacg ctcctgtgcc agcccgcgac
    18181 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    18241 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    18301 agcccaacaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    18361 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    18421 gacgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    18481 ccagtaatgc cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga
    18541 taaacgcttc aagtacagga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca
    18601 ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga
    18661 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg
    18721 ccagcaggcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg
    18781 tgatactgct atccagtgga tcaagcggac gtttgttatc gctacgggaa aactcttcga
    18841 tgttcacacc agcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    18901 gcaggttatc tggcgtcaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag
    18961 aaaggagata aatgtagtcg cgcccaagct tgccccatga ggccagcagc gggttgccga
    19021 catcctgttc accatcgctg ttaaagagct gcccggcatt ttcgctgtcg cgaaatagcg
    19081 gtaattcgcg atcttcaaaa ctgtgtcggc gctggcgagt cagtagtttc gccagataag
    19141 caggatcttt aatgtcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    19201 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    19261 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    19321 cgataaagcg ctgatagaga ttggcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    19381 attccaccag cgccttccac aacggcgctt gccaggcctg tgcctctccc agcccttcaa
    19441 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    19501 acaggtccgc cgcttttgag gaaagctgga acagctttcg cttgtcgcta tcgtcagtca
    19561 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    19621 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc
    19681 ggacgaacat atcccagata aagctcgcag gcagcggaaa atcaatgttt gccgcaatac
    19741 caaacttttg cgacagggtc atttgcagcc actgtgccat accggtactt tgcaccagaa
    19801 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    19861 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    19921 gacgattcgg gcaatgcagg cgggtcatct cgcctgctct gcccccgggt gaaactagcg
    19981 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    20041 caggtggcga aatcgcgcgc aattgcgttt gctgccaacc atgccgccag agctgttggt
    20101 actggtttct gctggtaaga ctgttcatta atgttcgctg gaaacccgat aacgcagtga
    20161 caatcatcac catcaacacc atcgccaaca ttacctccgg caggctaaag ccttgttgat
    20221 tcttcaggga agctgacata acgccccctc tttcagcgga caaaaatcgc tccagccgcg
    20281 tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    20341 ataaccggca attaataagg cttcattatc ttccagtaaa cgcaaacaaa cccgggcacc
    20401 agtggcagcg tacagtaagc actgaactgc gggctgcgtc tgccaggagt gcattttccc
    20461 ccacgccaac gccgactgaa cgatggcctg gcggcgcagt gactgacttt ccatgctcac
    20521 gcgagaggca aaactgcgat cctgctggct cattccttgt aatagcaagc tacccaaaac
    20581 cagcagcatc aggaccagag ccagtgacga aacacccttt tcgcggttca caggttgaat
    20641 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    20701 atattaaccg tcaacaccag cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    20761 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    20821 gcaccgcgta gcgtttccaa cacttgctcc ttcagacgaa atccaatctg gtcggactct
    20881 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    20941 tcaccctgtc cgacaatttc cagcccttcg ccggtacaga tgccatgaca ataacccgcc
    21001 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc
    21061 ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccaac
    21121 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    21181 tttacaggca tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa
    21241 ccaccagcca ccactcgccc gttgagtttt tgaagccaat atgcccggcc catgcggtat
    21301 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    21361 gtggcacaaa gaccaatggt gaactgccat gacaggtatt cgccccagca acggaactca
    21421 caaggcacca taacgtcccc tccctgataa aactgatact gtggtcgcgg ttatgccagt
    21481 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc
    21541 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    21601 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    21661 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    21721 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    21781 ggagggtaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga
    21841 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    21901 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    21961 gttcacggct taattgcaga tgagtttgat ccatatgatt actgtacagg tgcgtatcgc
    22021 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    22081 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    22141 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    22201 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgggaat
    22261 ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatctg
    22321 gcgttggcca ggcgcgccac tgtttaccat acactggtcc gaggtcgccg ttttcatcgg
    22381 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct
    22441 gcagaaacca cagcagttca tggatgatgg aacgcaggtg gcaacgttta gttgtcacca
    22501 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    22561 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    22621 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    22681 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    22741 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    22801 acgcgccgta gccaatcagg aacaagccag acacggctcc catcgggcgc ggtttacgaa
    22861 tatacaggtt gaggataata aacagcacca caccttccag cagcagctcg taaagctgtg
    22921 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    22981 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    23041 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc caaacgcccg gcaccaagac
    23101 caaacggaat gagtggtgcg ataaaatcag aaacctggaa gaaggaacgt ttagtacggc
    23161 gggcaaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    23221 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    23281 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    23341 caacttcatt tttggtccag ccgctgcccg gacgattcgc ccgtcgtgtt gccagccaca
    23401 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    23461 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    23521 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    23581 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    23641 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    23701 gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac
    23761 gctgcgcaag attttctgct tcggcataat caatgcgccg cagcaggtat tttacccgcg
    23821 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    23881 acatgggatc gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt
    23941 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg
    24001 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    24061 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    24121 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    24181 gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctt gtgaccatcg
    24241 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    24301 tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat
    24361 tctcttcgct aatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    24421 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    24481 cttccgacgg aaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    24541 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    24601 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    24661 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    24721 gatcgaccag caattcaccg cgatagccat cgacgatcag cgtccgacga tgcagcaccg
    24781 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    24841 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    24901 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    24961 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    25021 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt
    25081 caatgaccgt ttttaccgcc cattctgcca ccgagccttt atcaacctcg gcaaacaatt
    25141 cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt
    25201 ctttttgtgc accggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    25261 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    25321 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgct acaccaggtg
    25381 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacaaggca gtcaactgcg
    25441 actgagaaag aatcgctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    25501 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    25561 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt
    25621 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    25681 ggccgacgat cccttcatca aaggcgagcg ttacagtgcg accgcgtggt tttttcagcc
    25741 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    25801 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    25861 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    25921 cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca
    25981 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    26041 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    26101 tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag agaaaccatt
    26161 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    26221 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    26281 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    26341 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    26401 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    26461 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac
    26521 tctctgcagg gcattacaca ctaacaacaa ttgaataact cacagttatg tgcagagtta
    26581 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    26641 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
    26701 tttaaaatat tcattattag tcaatgcatt acatgtaatt taagtaatga aaaagagtaa
    26761 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
    26821 ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg
    26881 cacgtcaagg ctgtaaatgg aaacagtagt ggaggttttt cacagttatc ccagctttct
    26941 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacccgttg
    27001 cagtgttgcg caactcgatt accggcaacc taaaaagcaa tataaatcag ataattgaac
    27061 aacgcatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
    27121 accaaggtat catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga
    27181 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
    27241 tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
    27301 ggagatctgg ctaggtgcca gcgcagggag taaacctgaa caagattttg ctgctctggg
    27361 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
    27421 ttgtgttgcc ccgttaacgg gcaatagcgg agtgacctgg gaaaccagcc acgtgcgcca
    27481 caaactcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca
    27541 gcgtcgcgtt ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc ggcagctacg
    27601 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggattaccgc
    27661 tcgtcacggg gagtatttac agatacgacc gaaagcagcg aatgcgaaag cgcttaccga
    27721 agccattggt gcacggggcg aacggattct gacgctgccg cgcggctttt atttgaagaa
    27781 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
    27841 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
    27901 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
    27961 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gctccccaca
    28021 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
    28081 ctgctggcat caatcgcctg ggttacgcgc ctgacgaatc cgctttttac aatattcagt
    28141 caggaaattt ctgcccgtga cttgattctg cttctgggtg gcttgttcct tatctggaaa
    28201 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
    28261 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac
    28321 tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc cgccgtggtg
    28381 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgagcgccat
    28441 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg
    28501 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
    28561 gcggttgaaa gcctcaacct gattcgcaac aaaaagaacc cgctctgata ctccgtacgc
    28621 tctcctgcgg gagggcgtac tcacttctgt taccctcaca gtttaagatt ttcctgcttt
    28681 caagatatat aacgtcagtt tataaacaga ctattatcat aggtgggcca aatgaggata
    28741 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
    28801 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
    28861 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
    28921 tgcaaattaa ccgtgatgat gtttcgcaaa taattgaacg ttaacaaata aggtcagcat
    28981 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
    29041 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
    29101 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
    29161 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
    29221 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
    29281 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
    29341 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
    29401 ccgcgaagcg ctgcatgaca gcctcaagcg tctgcagact gattacctcg atctttatca
    29461 ggtgcactgg ccgcagcgcc caaccaactg cttcggcaaa ctcggttata gctggacaga
    29521 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgagc
    29581 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
    29641 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
    29701 gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact
    29761 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
    29821 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
    29881 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctgg cccctgctca
    29941 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
    30001 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
    30061 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
    30121 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
    30181 gccagcgcaa atagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
    30241 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
    30301 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
    30361 agcggaacga caaaaaagcc ccccaacacg ccaatcagca tcaacaaggc ataggctggc
    30421 agctgctcgt gttgcaggga gaaaatcagt accaccacgc caatcaaaat tccggctggc
    30481 atacagcgtg acacggtttc cagcgtaacc agcttcgctg ccgcacctgc gccaaccacg
    30541 ataccaatcg ctaccatcgc gttgagatag gtcggcgtag cgttatcggt aatgcccagc
    30601 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
    30661 ctggtgccca ccagcgaaaa acgtgtttca ccattgcgcc atagcgaggt gcaggcattc
    30721 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
    30781 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
    30841 gcgaccagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
    30901 gctatggtag aagcttccat taaaccgtta gcttttacta acttactacc cgtggttaat
    30961 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
    31021 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
    31081 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
    31141 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
    31201 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
    31261 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
    31321 tgcactgact cactcatcgt gttgttccac ttcgtctacc cagcttttca acgtgacaaa
    31381 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaaa tagcgaatat cacgcggtac
    31441 agcaagctcc ggcacgccgt gctcacgggc atactgttgc aacttatcgc gcgtcagttc
    31501 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
    31561 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
    31621 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
    31681 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
    31741 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
    31801 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
    31861 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
    31921 gttgatagaa acgacaggcg cgcattcggt cacaccgtag ccttcaagga tgcgcaggcc
    31981 aaatttatcc tgccaaagct gtttggtact ttcttgtagt ttttctgcgc ctgccaccac
    32041 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
    32101 cgaagtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
    32161 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
    32221 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
    32281 gaagtcggca atcgttttaa tctgctcgac attcgccaga atgcttttat ggctatggac
    32341 gacgcctttt ggatggcctt cagaaccgga ggtaaaaagg atcagcgcct cttcttccgg
    32401 ctgctgttta acctgtgcca gacgcggcat cagcaaatga gcgaagatcc atactttgtc
    32461 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgtacct gagtaagttg
    32521 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
    32581 gatttcagcc gccgtaatag cactggtcag cccttttacc ccagcagtgt agttcatcat
    32641 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
    32701 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
    32761 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaaattga cgtcttcgac
    32821 acatttcttc cctgctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
    32881 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
    32941 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
    33001 atgcagagta atttgcggga acaagcgatg tttaaccaga cctttcaggc ggctgaagtg
    33061 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
    33121 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
    33181 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
    33241 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
    33301 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
    33361 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
    33421 ctgggggtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
    33481 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
    33541 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
    33601 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
    33661 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
    33721 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
    33781 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
    33841 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg
    33901 gtcaatcgca acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
    33961 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
    34021 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
    34081 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
    34141 aataattcac ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct
    34201 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
    34261 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
    34321 caggtggctt atcacaccgg taatttctta ttgattggca atggttacca caacgaacaa
    34381 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
    34441 gccaaaatga tcccggatgc cgatttagcc tcattattga aacaaatgcc cggtatggtg
    34501 ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta ttgctctgga cgatcgttac
    34561 ggtgcctggc tggctacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg
    34621 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
    34681 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc
    34741 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
    34801 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
    34861 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
    34921 ccgcgcctga ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg
    34981 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
    35041 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
    35101 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
    35161 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta
    35221 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc
    35281 cttcacttcc ggcaaggcgc ggggttcaac atttccccct tcctgctggg taaagacatc
    35341 gcccgattca cataacggtc cggcgacgac ggtttccacc gttggtgcgt gttccagaga
    35401 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
    35461 cagatcgtta aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
    35521 ccgcacctga gtaattaaca cgccagactg cgctaccagg aagcgacccg gttcaatttc
    35581 cagtttcaca gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc
    35641 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
    35701 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc
    35761 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
    35821 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
    35881 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
    35941 cggattaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgaacat
    36001 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
    36061 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
    36121 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
    36181 cattaagcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
    36241 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
    36301 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
    36361 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
    36421 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga
    36481 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
    36541 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
    36601 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg
    36661 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
    36721 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
    36781 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
    36841 ctttctggta cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
    36901 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
    36961 tgcgggaaca gaacgtaccg aattactctc tttagatgaa ttgtgtgtgt tacctccggg
    37021 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
    37081 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
    37141 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
    37201 ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg
    37261 tttagtggtg cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
    37321 gcaccgcccg tcatcagcgc tggtgcaggc gtttagtgag catttacaag cggggttacc
    37381 gaaactggtc acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca
    37441 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctggc
    37501 accagtaaac caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg
    37561 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
    37621 tcttcaaaaa taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg
    37681 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
    37741 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
    37801 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
    37861 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
    37921 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
    37981 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
    38041 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
    38101 attgttttca ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag
    38161 acaaacgcct caacggccag gtgcccgatc tctattaacg aaaaaagggc cggatgtaca
    38221 gcacatccgg cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt
    38281 tgcgttcgat atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc
    38341 ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat
    38401 caagcagtgt caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg
    38461 aacaggtaat accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca
    38521 ccactggcgc ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga
    38581 gccaggacaa gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca
    38641 ccagagtgcc taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt
    38701 tatctaccgt aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag
    38761 tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcat
    38821 agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa
    38881 acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgcct tgttttaact
    38941 tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca
    39001 tacgcaacac ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat
    39061 ttggcaggaa gaccaccaga ataatcagca ggactgctgg taaagcaaga acccccaaca
    39121 ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc
    39181 cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca
    39241 tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca
    39301 cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaactgagta
    39361 caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac gacagccaac
    39421 cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca
    39481 aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga
    39541 tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac
    39601 gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt
    39661 tttcctgcca gcagagagta agacatagtg aaaaaatacg tgaacaattc acacaggtgt
    39721 caggtcggaa acagcataaa tatggattaa attgctgcga catgccgtta tgtgatggat
    39781 attccaattt taaaa
//
LOCUS       DBFIFS010000021        39099 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.021, whole genome shotgun sequence.
ACCESSION   DBFIFS010000021 DBFIFS010000000
VERSION     DBFIFS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.021"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(65..964)
                     /gene="lysO"
                     /locus_tag="N7A07_000995"
     CDS             complement(65..964)
                     /gene="lysO"
                     /locus_tag="N7A07_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU2475711.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(1422..2117)
                     /gene="aqpZ"
                     /locus_tag="N7A07_000996"
     CDS             complement(1422..2117)
                     /gene="aqpZ"
                     /locus_tag="N7A07_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU2475712.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            2543..4201
                     /gene="ybjD"
                     /locus_tag="N7A07_000997"
     CDS             2543..4201
                     /gene="ybjD"
                     /locus_tag="N7A07_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU2475713.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGHAD"
     gene            complement(4198..5154)
                     /gene="ybjX"
                     /locus_tag="N7A07_000998"
     CDS             complement(4198..5154)
                     /gene="ybjX"
                     /locus_tag="N7A07_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU2475714.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            5305..6420
                     /gene="macA"
                     /locus_tag="N7A07_000999"
     CDS             5305..6420
                     /gene="macA"
                     /locus_tag="N7A07_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU2475715.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            6417..8363
                     /gene="macB"
                     /locus_tag="N7A07_001000"
     CDS             6417..8363
                     /gene="macB"
                     /locus_tag="N7A07_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU2475716.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(8436..8660)
                     /gene="cspD"
                     /locus_tag="N7A07_001001"
     CDS             complement(8436..8660)
                     /gene="cspD"
                     /locus_tag="N7A07_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU2475717.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            8810..8884
                     /gene="yljB"
                     /locus_tag="N7A07_001002"
     CDS             8810..8884
                     /gene="yljB"
                     /locus_tag="N7A07_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU2475718.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            8983..9303
                     /gene="clpS"
                     /locus_tag="N7A07_001003"
     CDS             8983..9303
                     /gene="clpS"
                     /locus_tag="N7A07_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU2475719.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            9334..11610
                     /gene="clpA"
                     /locus_tag="N7A07_001004"
     CDS             9334..11610
                     /gene="clpA"
                     /locus_tag="N7A07_001004"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU2475720.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(11956..12043)
                     /locus_tag="N7A07_001005"
     tRNA            complement(11956..12043)
                     /locus_tag="N7A07_001005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12007..12009),aa:Ser,seq:gga)
     gene            complement(12297..12515)
                     /gene="infA"
                     /locus_tag="N7A07_001006"
     CDS             complement(12297..12515)
                     /gene="infA"
                     /locus_tag="N7A07_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU2475721.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(12800..13504)
                     /gene="aat"
                     /locus_tag="N7A07_001007"
     CDS             complement(12800..13504)
                     /gene="aat"
                     /locus_tag="N7A07_001007"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU2475722.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(13546..15267)
                     /gene="cydC"
                     /locus_tag="N7A07_001008"
     CDS             complement(13546..15267)
                     /gene="cydC"
                     /locus_tag="N7A07_001008"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU2475723.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(15268..17034)
                     /gene="cydD"
                     /locus_tag="N7A07_001009"
     CDS             complement(15268..17034)
                     /gene="cydD"
                     /locus_tag="N7A07_001009"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU2475724.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(17157..18122)
                     /gene="trxB"
                     /locus_tag="N7A07_001010"
     CDS             complement(17157..18122)
                     /gene="trxB"
                     /locus_tag="N7A07_001010"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU2475725.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            18667..19161
                     /gene="lrp"
                     /locus_tag="N7A07_001011"
     CDS             18667..19161
                     /gene="lrp"
                     /locus_tag="N7A07_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU2475726.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            19296..23324
                     /gene="ftsK"
                     /locus_tag="N7A07_001012"
     CDS             19296..23324
                     /gene="ftsK"
                     /locus_tag="N7A07_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU2475727.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            23479..24090
                     /gene="lolA"
                     /locus_tag="N7A07_001013"
     CDS             23479..24090
                     /gene="lolA"
                     /locus_tag="N7A07_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU2475728.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            24101..25444
                     /gene="rarA"
                     /locus_tag="N7A07_001014"
                     /gene_synonym="mgsA"
     CDS             24101..25444
                     /gene="rarA"
                     /locus_tag="N7A07_001014"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU2475729.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            25535..26827
                     /gene="serS"
                     /locus_tag="N7A07_001015"
     CDS             25535..26827
                     /gene="serS"
                     /locus_tag="N7A07_001015"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU2475730.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            27066..29510
                     /gene="dmsA"
                     /locus_tag="N7A07_001016"
     CDS             27066..29510
                     /gene="dmsA"
                     /locus_tag="N7A07_001016"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU2475731.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            29521..30138
                     /gene="dmsB"
                     /locus_tag="N7A07_001017"
     CDS             29521..30138
                     /gene="dmsB"
                     /locus_tag="N7A07_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU2475732.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            30140..31003
                     /gene="dmsC"
                     /locus_tag="N7A07_001018"
     CDS             30140..31003
                     /gene="dmsC"
                     /locus_tag="N7A07_001018"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU2475733.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(31039..31665)
                     /gene="ycaC"
                     /locus_tag="N7A07_001019"
     CDS             complement(31039..31665)
                     /gene="ycaC"
                     /locus_tag="N7A07_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU2475734.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            31980..33128
                     /gene="ycaD"
                     /locus_tag="N7A07_001020"
     CDS             31980..33128
                     /gene="ycaD"
                     /locus_tag="N7A07_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2475735.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            33338..34768
                     /gene="ycaM"
                     /locus_tag="N7A07_001021"
     CDS             33338..34768
                     /gene="ycaM"
                     /locus_tag="N7A07_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU2475736.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(34769..34879)
                     /locus_tag="N7A07_001022"
                     /pseudo
     CDS             complement(34769..34879)
                     /locus_tag="N7A07_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(34978..35718)
                     /gene="pflA"
                     /locus_tag="N7A07_001023"
     CDS             complement(34978..35718)
                     /gene="pflA"
                     /locus_tag="N7A07_001023"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU2475737.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35910..38192)
                     /gene="pflB"
                     /locus_tag="N7A07_001024"
     CDS             complement(35910..38192)
                     /gene="pflB"
                     /locus_tag="N7A07_001024"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU2475738.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38247..>39099)
                     /gene="focA"
                     /locus_tag="N7A07_001025"
     CDS             complement(38247..>39099)
                     /gene="focA"
                     /locus_tag="N7A07_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU2475739.1"
                     /translation="ADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFIS
                     IAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGR
                     ITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFI
                     EAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMG
                     IVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIY
                     LRENDHH"
BASE COUNT         9736 a         9597 c        10371 g         9395 t
ORIGIN      
        1 tgcaacttta agggagattg atttagcgca attttggcgg cagggatcta ccgccagaga
       61 ggtattacgc ggagaaaaag gcgatgagga tcggcactaa caggctaaga ataaaaccgt
      121 gaacaattgc cgccgggacc atatccagcc cgccagtacg ttgaagaacg ggcagggtga
      181 aatccatcga cgtcgcaccg cataagccca gtgcggtaga gcggctgcgg cgaatcagcc
      241 cagggatcaa cataattgca atcagctcac gggccagatc attaaaaaac gccgcgctcc
      301 cgattaccgg accaaaagat tcggtcaata aaataccgga aagagaatac cagccgaaac
      361 cagaggccat tgccagcgcg gtattgatgg gtagatcaag aataaaggcg ttaattaaac
      421 caccaattaa tgaactggca accaccacca cggcgacaat cattccccgg cgattaagga
      481 caatctgctt taaggtcatg ccattattgc gcaactgaat accgacgagg aaaagtagca
      541 gaattaacgt gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt ccactcaggc
      601 caatggcaaa accaatcact actacgccgc acagttttag cgactcaagc gccatcgcaa
      661 tacgcgacgg gagtttttct tgctgatggt ggttgcgcca cggcagaccg cgctccagcc
      721 acataagggc ggcaatatta cacagtaaaa taacggtaat actgacggca gaataatgca
      781 gaatcgccaa caggttactg gcgaggttat cgagaaacgc cagactgata cccataaaaa
      841 agagaataag gtaaaccatc cagcttaata gctgattaat aacttttaac gcagcttgtt
      901 ggcgaagcgg aatgaggtaa cccacaatca ggggaaccag aatgattaac aacccagaaa
      961 acatgaaaaa ccagcccttg caaagaaaga agtcgaaatg cgcgatgaca cactactgaa
     1021 agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcatcaga gggggatttt
     1081 taattctata tgagtgttta gcattgttgc gctcctttca tgtgcaatgc catataaagc
     1141 cactggcgca gggagcgcgc agggggcggc cagtcgccgc cgccccctgc acccccgggc
     1201 tctggcgaac aaaatcgccg ctgcgcggta ccttcagctt atccccttcg gcaaccgggt
     1261 cgggcgcgag gtaacgtcct gtaagacgcg ccctcagccc acatccatgt gggctgcccc
     1321 ggccttcggg gaacgcttca gcgattttga cgccaccaat acccgtgcgg tttgtaatgt
     1381 cgaagataaa acaaccaggg cagataaaat gccaatgcta attaattacg cttttccagc
     1441 agagtccggt aaatcagacc accgataatg ccaccgacaa ttggcaccac ccagaagaac
     1501 cacagttgtt ctaatgccca gccgccctgg aagatggcaa ccgcggtgct gcgcgccggg
     1561 ttaacagaag tgttagtcac cggaatactg attaagtgaa tcagggttaa ggccagacca
     1621 atagcgatcg gcgcaaaacc tgccggagcg aatttgtcgg ttgcaccgtg gatcactaac
     1681 aggaagcccg cactcaatac cagttcaact accagcgcgg aaagcatgga ataaccgcct
     1741 ggtgaatgct cgccataacc gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt
     1801 ttaccactgg caattaaata cagcagcgct gccgcaacaa taccgccgac aacctgggca
     1861 attacgtagc caacgacttc ttttgccgga aaacgtccgc cagcccataa accaatagtg
     1921 accgccgggt taaaatgacc accagaaata tgaccaacag caaaggccat cgtcagaacg
     1981 gtcagaccga acgccaacgc cacgccggca aaaccaatgc ctaattccgg gaagcctgcg
     2041 gccagtacag cactaccaca gccaccaaaa acaagccaga aagtaccaaa acattcagct
     2101 gctaattttc tgaacatatc cacctcaatt aaaaattgac cctgtgaaaa atatggtcgt
     2161 tttatagggt cgtcgtaaaa aagtgacgac ggaaataatg cgcggctatt ttaaaaacga
     2221 aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca acagcgggtt
     2281 gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt taatccttta
     2341 ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt tgcagactta
     2401 tcagcaagag ggagtataac gcgattattc gctcattttt cagacatttg ccatgcttaa
     2461 atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgccagcg taaaggataa
     2521 gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg
     2581 tatcaaccgt ttgtcgttga tgctggaaca aaacaacgtc ctgattgggg agaacgcgtg
     2641 gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat cagatctcta
     2701 ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc gagaacatca
     2761 tctgcatatt attttgacct tccgcgaatc gctgccaggt cgacatcggg ttcgccgtta
     2821 tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta ttttttatcg
     2881 tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa
     2941 agacggacat ccgattgatg ttgaggatat taacgatcag gcacaccatc tggtgcgttt
     3001 aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta acggcacggt
     3061 gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt
     3121 gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt ccgcaatggt
     3181 gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat atcgtttaat
     3241 gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca tcaaccggat
     3301 gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt ttgctacttt
     3361 attgcaggca aaaggcacat tgcgactgga taaagacgcc cgtccattgt tgctgatcga
     3421 agatccagaa acgcgtttac accccattat gctttcagtt gcctggcatc tgttgaatct
     3481 tttgccttta caacgtattg ctaccaccaa ctctggcgag ctgctttcgt taactccagt
     3541 ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc
     3601 aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt ttaatcgtcc
     3661 gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa cctgggttat
     3721 caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca aggtcattga
     3781 gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg ggattgaatg
     3841 gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg tacgcagcct
     3901 gttgaataat gatcgggaag ccgaacgaga acatttaacg gcgttaccgg cgctggatat
     3961 ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg cgcaaatccc
     4021 ggaaaatgtc ccgatgaatc tgcgcaaaat tatctcgaaa gcgatccatc gctcttccaa
     4081 acccgatctt gccattgaag tggcaatgga ggccggacgt cgtggtgtgg actccgtacc
     4141 gacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc acgcggatta
     4201 accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat agcgccgacg
     4261 gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg
     4321 aaggcggtaa tggcgttcag catcacatac gccgccaacc gattcccaga aagcgttgta
     4381 atcggcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc
     4441 attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac aggccgcttc
     4501 catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct ggatttcctg
     4561 atgtggaatt tcccattttg cgccttgcag tccaccaata aacatcgttc ttttcccctg
     4621 atattcacac agggtaaacg tgatttctgc cagaggaata ccttcgctgt tgcggaacag
     4681 gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa gcgtgaactg
     4741 ctcgccgttt ttgccttcca gcttcgccag ttgcagcccg ggggtattca aatataagct
     4801 gaattcttcc gccgacatac atccacggag taacgcataa tggtaacgta acgcctccag
     4861 caattgctta cggctaagat tcgccgcaag gtaagggcga tgcagacgca caggcagtcg
     4921 cggctggcgc gttaacaata cattgagatt aggccagtgg gaaagttcgt tcatccactc
     4981 aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa aactacggcg
     5041 atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct
     5101 gctgagagat tcagatgacg taaaggtccg ttcggttagc tgcgacatat tcatgaaatc
     5161 aatggttgta catgacgtcg atttcaccat tgcgtatctt aaccaaacat caatagtgtg
     5221 attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg acccggattg
     5281 gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt
     5341 attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct taacgcaccc
     5401 gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg
     5461 accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg
     5521 aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt aggggttatt
     5581 gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat ggagctacgc
     5641 gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta ttcccgtcag
     5701 caacgtctgg cacaaacgca ggcggtttca ctgcaggatc tcgacaccgc cgcgacggag
     5761 atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg caatcaggct
     5821 tctctcgata cggctaaaac caatctcgat tacactcgca tcgttgcccc gatggccggg
     5881 gaagtcacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca acaagcaccg
     5941 aacattctga cgctggcaga tatgagcacc atgctggtaa aagcgcaggt ttctgaagcg
     6001 gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtacttgg cgatccactg
     6061 acgcgctacg aggggcaaat caaggatgta ctaccgacgc cggaaaaggt taacgacgct
     6121 attttctatt acgcccgttt tgaagtcccc aaccccaatg gtttgctgcg gctggatatg
     6181 actgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgctgacgat ccctctgtcg
     6241 gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg taatggtgaa
     6301 acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga gattgtcaaa
     6361 gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc tgcacaatga
     6421 cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat gagcaggttg
     6481 aggtgctgaa gggcatcacc ctcgatattt atgccggtga gatggtagcg attgttggcg
     6541 cttcgggttc cggtaaatcg accctgatga atattctcgg ctgtctggat aaggccacca
     6601 gcggcaccta tcgcgtcgcc ggtcaggatg ttgccacgct ggacgccgat gcgctggcgc
     6661 aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt tcgcatttaa
     6721 ccgccgagca gaacgttgaa gtaccagccg tctatgctgg tcttgagcgg aaacagcggc
     6781 tgcttcgcgc ccaggagttg ctgcaacgac tggggctgga agaccgtaca gagtattatc
     6841 cggcacagct ttcgggtggt cagcaacagc gcgttagcat cgcgcgggca ttgatgaacg
     6901 gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat tcaggcgaag
     6961 aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt atcgtcactc
     7021 acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac ggcgaaattg
     7081 tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggaa cctgttgtca
     7141 acacggcgtc tggctggcgg cagtttgtca gcggttttaa tgaggcgctg acgatggcat
     7201 ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg attataatcg
     7261 gtattgcgtc ggtggtttcc attgtcgtgg tgggcgacgc cgccaaacaa atggtgctgg
     7321 cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa gattttggcg
     7381 atgacgatcc gcaatatcaa caggcgctga agtacgatga cttaatcgcc atccaaaaac
     7441 aaccgtgggt cgcttcagcc acacctgccg tctcgcaaaa cctgcgcctg cgttataaca
     7501 atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat gtctatggca
     7561 tgaccttcag tgaaggaaac acctttaacc aggaacagct gaacggtcgt gcgcaggtcg
     7621 tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat gtggttggcg
     7681 aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt
     7741 cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg atgtccgggc
     7801 gagttatggg ccagtcgtgg ctgaactcca ttactgtcag ggtgaaagaa ggatttgaca
     7861 gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt aagaaggatt
     7921 tctttacctg gaacatggac ggcgtcttga aaaccgttga aaagaccaca cgtactttac
     7981 aactgtttat gacgctggtg gcggtgattt cgctggtagt gggcggtatt ggtgtaatga
     8041 atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc atggctgtag
     8101 gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg gtttgcctgg
     8161 tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg cagcttttct
     8221 tacccggctg ggagattggt ttttcaccgt tggcactgct gctggcgttt ctctgctcga
     8281 cggtcaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga ctggatccag
     8341 tagatgctct ggcacgagag taatttttga gataaaaatg ccagccgatc gggctggcat
     8401 tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg
     8461 acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg
     8521 gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa
     8581 atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac
     8641 ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga
     8701 ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta
     8761 gagaattagg gcgagccgtc aagcatttga caggggacag ggggcaggta tgaatcaaaa
     8821 atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag
     8881 atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca
     8941 gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac
     9001 tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct
     9061 atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg
     9121 ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac
     9181 taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg
     9241 atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc
     9301 tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt
     9361 ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac
     9421 ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg
     9481 gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg
     9541 cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa
     9601 cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg
     9661 gtcgctatct ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc
     9721 agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag
     9781 tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg
     9841 gagaatttca cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt
     9901 ggtcgtgaga aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac
     9961 ccgctgctag tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg tcttgcctgg
    10021 cgaattgttc agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat
    10081 atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg
    10141 ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc
    10201 attatcggtg cgggtgcagc gtctggtggt caggttgatg cggctaacct aatcaaaccg
    10261 ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac
    10321 attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg
    10381 tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcacaccac
    10441 gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt
    10501 aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcggg cgctcgcgca
    10561 cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg
    10621 gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg
    10681 aaaaacctcg gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg
    10741 ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt
    10801 tcgttcctgt ttgccggccc taccggggtc gggaaaacag aggtgacggt acagctttcg
    10861 aaagctttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc
    10921 gtcagccgtc ttattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg
    10981 actgatgcgg tcatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg
    11041 cacccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac
    11101 aacggacgca aagcggactt ccgtaacgtg gtactggtga tgaccactaa cgccggggta
    11161 cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg
    11221 gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg
    11281 ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg
    11341 caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg
    11401 ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag
    11461 gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt
    11521 caggtcaccg tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca
    11581 caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg
    11641 taatctcgaa agaggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt
    11701 ttggaggtga aggtaatttg ttgataatta gtgctgctgg aaaaggtaag gataaaaaag
    11761 ggtgacagca ggaaaatgag atggttttgc tttattaaca acgggctaaa cgtgtagtat
    11821 ttgagttcac tgccgtacag gcagcttaga aattcactgg taacatactc cacccgccca
    11881 ccatgttcac tgccgtacag gcagataaaa tgcgaaaaaa aagcccgtac tttcgtacga
    11941 gctcttcttt aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac
    12001 acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctgcca
    12061 aacctcatgg gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga
    12121 attttaatga taattattgt ttgctcatac tgtaaacaac ttgtgcagta tatctacatc
    12181 gagacaagtt atggacttat acttccaaag tacttcatgc atatcacaaa ataaaaaggc
    12241 cggttaaacc gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag
    12301 cgactacgga agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact
    12361 ttgtcgcccg tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta
    12421 accacgtgac cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg
    12481 gtaccttgca tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg
    12541 taatttgaac cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa
    12601 atcgcaacaa acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag
    12661 cgcaacgtat aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct
    12721 tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg
    12781 agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa gcatcgtggt acccagaaat
    12841 tattcggcaa tcgtccgagg cgcatttgat tgagataatt aaggtaattc cggcggggaa
    12901 tttcgcaggc accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac
    12961 cgccatgacc gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca
    13021 tccggctgaa catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc
    13081 cgacaagctc atcttcacgc cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc
    13141 gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac
    13201 agccttcaat gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat
    13261 gaaatcgctt catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat
    13321 cgggcgacca ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt
    13381 aagccattaa caggcgcgca gggctaagat cgcccccaag tgccagcagg ccgttaggct
    13441 cacgtaatgc gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc
    13501 gcatgaccgc aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt
    13561 gaactggtaa taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat
    13621 ttgcccgttg tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt
    13681 gaccattaac accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc
    13741 ggttgtggca tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa
    13801 cagcgcacgg gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc
    13861 acctaaccaa ctgttgagac ctgcatcctc gagctgcttc tccagaccaa cgcgacgtaa
    13921 gatcaccgcc agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag
    13981 cgtggcgcta aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc
    14041 ttcattcagg ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca
    14101 tgcgcgggtc agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc
    14161 tatatgttcc ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc
    14221 cggataagtg aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg
    14281 ggtatcaggg aaggtgacct ccggtttttg atccgttaag tcagagatac gtacggcaga
    14341 ggcaatgact tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc
    14401 ggctaacgcg caaaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac
    14461 gccgccagac gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat
    14521 cgcttgcgac aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc
    14581 tgtattctct agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg
    14641 cccttgcagc caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg
    14701 cccggtgctt tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat
    14761 aatgccgccc agcgtaaagg cgagggtgga atcaaggaaa cttaacccga ttgtcaccac
    14821 cataatcacc acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag
    14881 cgtatcaaca tccgccacca cgcggttgag caattcgccc tgacgatagc gcgccagtcc
    14941 ggcaggggag aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg
    15001 gaaagtcgcg tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc
    15061 tgcaccacgc acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc
    15121 cgcaaccgct gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag
    15181 cagcgtcaca attgccagca caataccaag acttaacatc catttatgac gtttatacag
    15241 tgccagatag ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt
    15301 ggcgaatggg ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatccggcc
    15361 atcctgcata acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat
    15421 taacgttgtc tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg
    15481 agcatcaagg ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc
    15541 acgggccacc gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac
    15601 aggcgtatca acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag
    15661 cgctgcttgt aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa
    15721 tgttgctgcc ggtaattgtg ggttttgacc aacccaggag agatgtttac gccatgattc
    15781 tggtgataaa tcgcgtaatt ctatcccgtt gattcgtaat gatccctgat atgagagaaa
    15841 accagaaagc gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc
    15901 acgttggcct gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga
    15961 cgtgataaac agatcctcgg cctcaatggt cagcggatcg gttgatgcta attccgtctc
    16021 accgcgctgc ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc
    16081 aacagcctgg gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc
    16141 tggcgcaagg atcagggcca gaaaacccgc agccagcgtc acgccagtat cgtagtgacc
    16201 aaaatccagc tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag
    16261 cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc agccgtagca cttccattgt
    16321 ccgttggcgg aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc
    16381 aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc
    16441 aagagcgaga aagttacgtc ggttagcatc ggcagccccc atcccaacca gcgccataaa
    16501 caacggaatt aacggtgcag tgcccagcag aatgagcgcc gcagcccagt tagaggggaa
    16561 gatcgccacc acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg
    16621 tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc
    16681 aggtttaccc tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg
    16741 gatggcaaag cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac
    16801 ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg
    16861 gggaatattc tccataatca tatgttgcag aattcgcgcc atgaaccatg cctgggcaat
    16921 gatcaatatg ccgctcacaa agcccagcag acgagaaata ttcagccaac gttgggagat
    16981 gacgctttgc tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg
    17041 cttagcaggt gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa
    17101 ggcgacccat agtcgcatgg tgtcgccttc tttacttttg ttactgattt gtaaaattat
    17161 tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta
    17221 ccagcagatg taatggcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg
    17281 ccaggaatgc tggtctgggt ggcattacca tgaatacccg actgcacttt gatgtagccg
    17341 ttttccagtt ccagctgccc ttcgaaaatg gcagtattcg ggctgtgacc gatagcaaca
    17401 aacagaccgg caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga
    17461 acgccagtga cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga
    17521 atgatgttgc cgttctccac tttatccatc agacgcttaa tgaggatttt ttccgcgcgg
    17581 aaaccgtcac ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc
    17641 tcttcaaccg cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa
    17701 ccgtcgcagg ttgcacaagc agaaactcca cggcctttaa aggcttcttc agagggcagg
    17761 ccgagatagc gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg
    17821 ttatcgccat tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa
    17881 atgatctcag tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg
    17941 gtcagatcgt ttggatcgcc aggccagttt tccacttccg tggtggtggt cagttggccg
    18001 cctttttcca tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc atagacagca
    18061 gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc
    18121 atgagatccc catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta
    18181 aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt
    18241 caaattctcc cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa
    18301 atgatgaata aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt
    18361 tgctttgaca atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc
    18421 ttatatgcgc ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta
    18481 tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac
    18541 cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag
    18601 cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata cagagagaca
    18661 ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac
    18721 attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg
    18781 ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt
    18841 cagggctata cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt
    18901 gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa
    18961 aaacttgaag aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa
    19021 acacgcgtgc cggatatgtc agcctaccgt aagttgctgg gggaaaccct gctgcgtctg
    19081 cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt
    19141 ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact
    19201 cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat
    19261 cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca
    19321 aagaagtcac attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc
    19381 ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc
    19441 ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg
    19501 cgtggttggc agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca
    19561 ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa aacattgatt
    19621 attttgccgt ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc
    19681 tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct
    19741 tactaagcac tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct
    19801 gcgtttgggc agcgggcctg acgttgttca ccggttggtc atgggtgacc attgctgaaa
    19861 aactcggcgg ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg
    19921 atacctgggt cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaaccacg
    19981 gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac
    20041 ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg
    20101 gtaagcggat ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc
    20161 cggacgacgt cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc
    20221 cattattaaa cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg
    20281 cgacacaaag ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg
    20341 ttgatgttcc accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg
    20401 gagagccggt tattgctcct gcaccggaag gttacccaca gcagtcacaa tatgcgcagc
    20461 ctgcagtgca atataatgag ccgctgcaac aaccagtaca gccgcagcag ccgtattatg
    20521 cacctgcagc tgaacaacct gcgcaacagc cgtattatgc ccctgcgcca gaacaaccgg
    20581 tggcaggtaa cgcctggcaa gccgaagagc agcaatccac ttttgctcca cagtctacat
    20641 accagactga gcaaacttat cagcagccag ccgctcagga gccgttgtac caacagccgc
    20701 aacccgttga acagcagcct gttgtggagc ctgaacccgt tgtagaagag acaaaacccg
    20761 cgcgtccgcc gctttactac tttgaagaag ttgaagagaa gcgagcccgt gaacgtgaac
    20821 aacttgcggc ctggtatcaa ccgattccag aaccggttaa agaaccagaa ccgatcaaat
    20881 cttcgctgaa agcaccttct gttgcagcag tacctccagt agaagccgct gccgctgttt
    20941 ctccgctggc atctggcgtg aaaaaagcga cactggcgac gggggctgcc gcaaccgttg
    21001 ccgcgccagt cttcagtctg gcaaatagcg gtggaccgcg tccgcaggtc aaagagggga
    21061 ttggcccgca gttgccacga ccgaaacgta tccgcgtgcc aactcgtcgt gaattggcgt
    21121 cttacggtat taagctgccc tcacagcgtg cggcggaaga aaaagcccgt gaagcccagc
    21181 gcaatcagta cgattctggc gatcagtaca acgatgatga aatcgatgcg atgcagcagg
    21241 atgaactggc acgtcagttc gcccagacac agcagcaacg ctatggcgaa cagtatcaac
    21301 atgatgtgcc cgtaaatgca gaagatgcag atgctgcggc agaggctgaa ctggctcgtc
    21361 agtttgcgca aactcaacaa caacgttatt ccggcgaaca accggctggg gcgaatccgt
    21421 tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg
    21481 aaccgctgtt tacgccaatt gttgaacctg tacagcagcc gcaacaaccg gttgcaccgc
    21541 agcagcagta tcagcagcca caacagccgg ttgcgccaca gccgcagtat cagcagccac
    21601 aacagcaggt tgcgccgcag ccacaatatc agcagccgca acaaccggtt gcgccacagc
    21661 agcaatatca gcagccgcaa caaccagttg cgccgcagcc gcagtatcaa cagccgcaac
    21721 aaccggttgc accgcagccg cagtatcagc agccacaaca gccagttgcg ccacagccgc
    21781 aggatactct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg ttgcataaac
    21841 cgacgacgcc gctgccttct ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg
    21901 tagatacctt tgcgcttgaa caaatggctc gcctggtgga agcgcgtctg gctgatttcc
    21961 gtattaaagc cgatgtcgtc aattactctc cggggccggt tatcactcgc tttgaactta
    22021 acctggcacc gggcgtaaaa gcggcgcgca tttctaactt gtcacgggac cttgcccgtt
    22081 cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc
    22141 tggagttacc gaataaaaaa cgacaaaccg tttatctgcg cgaagtattg gataacgcca
    22201 aattccgcga taatccatcg ccattaaccg tggtgctggg taaagatatc gccggtgagc
    22261 cggtggttgc cgatctggcg aaaatgccgc acttgttagt tgcggggact accggttccg
    22321 gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca cagccagaag
    22381 atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtgtat gaaggcattc
    22441 cgcacctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg ctgcgctggt
    22501 gtgttaacga gatggagcgt cgttataaac tgatgtctgc gctgggtgtg cgtaatctgg
    22561 cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt ccagacccgt
    22621 actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa gaaccataca
    22681 ttgtggtgtt ggtggacgaa tttgccgacc tgatgatgac agtaggtaaa aaagtggaag
    22741 agctgatagc acgtctggcg caaaaagccc gtgccgcggg tatccacctc gtactggcaa
    22801 ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt ccgacccgta
    22861 tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag gctggcgcgg
    22921 aatcactgct gggtatgggg gatatgctct actctgggcc gaactccacg ttgccggtac
    22981 gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag gactggaaag
    23041 cgcgtggtcg cccacagtat gttgatggca tcacctccga cagcgaaagc gaaggtggtg
    23101 cgggtggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg gtgcagtttg
    23161 tcactgaaaa acgcaaagcg tcaatttctg gcgtacaacg tcagttccgc attggttata
    23221 accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc gaacaggggc
    23281 acaacggtaa tcgtgaagtg ctgtccccac cgccgtttga ctaattaatg cattgccgga
    23341 taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta ttttcttctt
    23401 tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat cgggagtgac
    23461 gtaatttgag gaataatgat gaaaaaaatt gccatcacct gtgcattact ctcaagctta
    23521 gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct ggataaagtc
    23581 agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc ggcggtgcag
    23641 gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca tatgacacaa
    23701 cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa cccgttcgtt
    23761 gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt tatgctgatt
    23821 gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg cgatgacttt
    23881 gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa cgtgggacgt
    23941 gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag cagttatcaa
    24001 ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa
    24061 ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc tgtcgctcga
    24121 tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa atttagcaca
    24181 gtatatcggt cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc
    24241 cgggcattta cattctatga tcctctgggg gccaccgggt accggcaaaa caaccctcgc
    24301 tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg tcacctctgg
    24361 tgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg caggtcgccg
    24421 cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg atgcatttct
    24481 gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa acccgtcgtt
    24541 tgagcttaat tcggcactgc tttcccgtgc ccgtgtttat ctgttgaaat ccctgagtac
    24601 agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg gctatggtgg
    24661 tcaggatatt gttctgccag atgaaacacg acgtgccatt gcagaactgg tgaatggcga
    24721 cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag tcgatgatag
    24781 cggtaagcgg gtcctgaagc ctgaattact gaccgaaatc gccggtgaac gtagcgcccg
    24841 ctttgataac aaaggcgatc gcttttacga tctgatttcc gcactgcata agtcggtacg
    24901 tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga attattaccg ctggtggcga
    24961 tccgttatat gtcgcgcgtc gctgtctggc gattgcgtct gaagacgtcg gtaatgccga
    25021 tccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg ttggcccggc
    25081 ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc caaaaagcaa
    25141 cgctgtctac actgcgttta aagccgcgct ggccgatgct cgcgaacgcc cggattatga
    25201 cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg gctacgggca
    25261 ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg tttacttccc
    25321 gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg aaggcaagat
    25381 tggcgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa aacgctaccg
    25441 ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg ctgtggccac
    25501 atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc tgctgcgtaa
    25561 tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg atgtagataa
    25621 gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc
    25681 ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga
    25741 gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa aagccgagct
    25801 ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga
    25861 tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagccgct ggggtacccc
    25921 gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc actctggcct
    25981 cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga aagggcagat
    26041 tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg aacagcatgg
    26101 ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg gtacgggtca
    26161 gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag aagcagacac
    26221 cagtaactat gcgctgatcc caactgcaga agttccgctg actaacctgg tgcgcggtga
    26281 aatcatcgat gaagatgatc tgccaattaa gatgaccgcc cacaccccat gcttccgttc
    26341 tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc agttcgacaa
    26401 agttgaaatg gtgcagatcg tgcgcccgga agactcaatg gcggcgctgg aagagatgac
    26461 tggtcatgca gaaaaagtcc tgcagttgct gggcctgccg taccgtaaaa tcatcctttg
    26521 cactggcgac atgggctttg gcgcttgcaa aacttacgac ctggaagtat ggatcccggc
    26581 acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc aggcacgtcg
    26641 tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc ataccctgaa
    26701 cggttctggt ctggctgttg gtcgtacgct ggttgcagta atggaaaact atcagcaagc
    26761 tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac tggaatatat
    26821 tggctaatac ccaatttttc tgaatctaaa aagcgcctac gggcgctttt tttgtctccc
    26881 tttgataccg aacaataatt actcctcact tacacgtaat actactttcg agtgaaaatc
    26941 tacctatctc tttgattttc aaattattcg atgtatacaa gcctatatag cgatctgcta
    27001 tagaaataat tacacaatac ggtttgttac tggaatcaat cgtgagcaag cttgagtgag
    27061 ccattatgaa aacgaaaatc cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt
    27121 tggtaaaaac gacagcgatc ggcggcctgg caatggccag cagcgcatta acattacctt
    27181 ttagtcggat tgcgcacgct gtcgatagcg ccattccaac aaaatcaggc gaaaaggtta
    27241 tctggagcgc ctgtacagtt aactgtggta gtcgctgccc gctacgtatg cacgtcgtgg
    27301 acggtgaaat caaatatgtc gaaacggaca ataccggcga tgacaattac gacggcctgc
    27361 accaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg ccgtgtctac aatccggacc
    27421 gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta
    27481 gctgggaaga agcctacgac atcatcgcga ccaatatgca gcgcctgatc aaagagtacg
    27541 gcaacgaatc catctatctg aactatggca ccggtacgct gggtggcacc atgacccgct
    27601 cctggccgcc gggaaatacc ctggtcgcgc ggctgatgaa ctgctgcggc ggctatctga
    27661 accattacgg cgactactcc tccgcgcaaa ttgcggaagg tctgaactat acctacggcg
    27721 gctgggcaga tggcaacagc ccgtcggata tcgaaaacag taagctggta gtgctgtttg
    27781 gtaataaccc tggcgaaacg cgaatgagtg gcggtggggt gacttactat cttgaacagg
    27841 cacgccagaa atctaatgcc cgcatgatca tcatcgatcc gcgctatacc gacaccggtg
    27901 ccgggcgcga agatgagtgg atccctattc gtccgggaac agatgccgca ctggttaacg
    27961 gtctggcgta cgtcatgatc actgaaaacc tggtggatca ggcattcctc gataaatatt
    28021 gcgttggcta cgatgagaaa accctgccag ccagtgcgcc gaaaaatggc cactataaag
    28081 cttatattct gggtgaaggg ccagatggcg tggctaaaac accggaatgg gcctcgcaaa
    28141 tcaccggtgt tccggcagac aaaatcatca aactggctcg tgaaatcggc agtaccaaac
    28201 cggcgtttat cagccaggga tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc
    28261 gtgctatctc gatgctggcg attctgaccg gtaacgttgg tattaatgga gggaacagcg
    28321 gcgcgcgtga aggttcatac agtttaccgt ttgtccgtat gccgaccttg gaaaacccga
    28381 tccagaccag catttcgatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga
    28441 cggcgctacg tgatggtgtg cgcgggaaag ataagctgga tgtgccgatc aaaatgatct
    28501 ggaactatgc cggtaactgc ctgattaacc agcattctga aatcaaccgt acccatgaaa
    28561 tccttcagga tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat
    28621 cggcgaaata tgctgacatc ctgctgcctg actgcaccgc ttccgaacag atggactttg
    28681 cactggatgc atcctgcggg aatatgtctt acgtgatttt caacgatcag gtgattaaac
    28741 cgcgctttga atgtaagacc atctatgaaa tgaccagcga actggcaaaa cgtcttggcg
    28801 ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg gatgcggcat ctgtatgccc
    28861 agtcgcggga agcgattcct gaactgccaa cgtttgaaga gttccgcaag caggggatct
    28921 ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa agccttccgt gaagatccgc
    28981 aggcaaatcc actgaccacg ccatcgggta aaattgagat ttattcgcag gcgctggctg
    29041 acattgccgc tacctgggaa ttgccagaag gcgatgtgat cgatccactg ccgatctaca
    29101 cgccgggctt tgaaagttat caggatccgc tgaacaaaca gtatccgctg cagcttacag
    29161 gtttccacta taaatctcgc gttcactcaa cttacggcaa cgttgatgtg ctgaaagcgg
    29221 cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca aaaacgcggt atccacaacg
    29281 gcgataaagt caggatcttt aacgatcgtg gtgaggttca tattgaggcg aaagtgacgc
    29341 cacgaatgat gccgggtgtg gtcgcactgg gtgaaggtgc ctggtatgac ccggatgcaa
    29401 aacgtgtcga taagggtggt tgtattaacg tactgaccac tcaacgtccg tctcctctcg
    29461 ctaaggggaa tccgtcacat acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg
    29521 atgacaaccc agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc
    29581 gagctggctt gtaaagacta caaagatttg atgccagaag tcagcttccg ccgcatttat
    29641 gaatatgctg gtggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc
    29701 tactatctgt cgatttcgtg caaccactgc gaagatccgg cttgtactaa agtctgcccg
    29761 agcggtgcga tgcataaacg tgaagatggt tttgttgtgg tcgatgaaga tgtgtgcatt
    29821 ggctgccgct actgccatat ggcttgcccg tatggcgcac cgcaatataa cgaaacgaaa
    29881 ggccatatga ccaaatgcga tggttgttat gaccgtgttg cggagggtaa aaagccgatc
    29941 tgtgttgaat cttgtccgct gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa
    30001 aaacatggcg atctggcggc cgttgcgccg ttgccgcgag ctcactttac caaaccgaat
    30061 attgtgatca aacccaatgc caatagccgc ccgaccgggg ataccactgg ctatctggca
    30121 aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa tggccgctga tgatcttcac
    30181 ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg gctttggcgc tgcttaaagg
    30241 cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc atgtttggct tatgggtgct
    30301 gatgggcatt ggctttatcg cctctatgct ccatcttggt tcaccgatgc gcgcctttaa
    30361 ctcgctcaac cgggtagggg cttcagcact cagtaacgaa atcgccagcg gttcgatctt
    30421 ttttgctgta ggcggcatcg gctggttgct ggcaatgctg aaaaagctgt cacctgcatt
    30481 gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc gtctttgtct ggatgatggt
    30541 gcgtgtgtat aacagcattg ataccgttcc gacctggtac agcatctgga cgccgatggg
    30601 cttcttcctg acgatgttca tgggcggccc gttactgggt tacctgttgc tgagtctggc
    30661 tggcgtcgat ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt
    30721 gagtggcgtg atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca
    30781 gcaggccgca gcgctggtgc cggactatgg tgcgctgatg tcctggcgaa tcgtgctttt
    30841 ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc
    30901 gctactatcc gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt
    30961 ctatggtttg catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt
    31021 ttcggccccg gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt
    31081 acgataatca ggaatgtggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca
    31141 gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac ctgcttgcga
    31201 catgcgatcc catgccgaat gccgggtaat ttcattaaag gtccctgacg cgtcagtcac
    31261 aacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc
    31321 ggttaccaca ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa
    31381 atcttcgtta tcccaggcgt taatattgcc agggcgagca atgtaaggtg catcggggaa
    31441 ttgtgctttc agctctggaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag
    31501 aatagttggt aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa
    31561 cttatcgggt tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa
    31621 aacggcagca tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg
    31681 agagtcagtg gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg
    31741 ggaaatatga gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg
    31801 tgttagatca ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga
    31861 atgagtaata taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac
    31921 aatgtttttt gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca
    31981 tgtccacgta tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg
    32041 cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat
    32101 ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga
    32161 cagggtatgt cattaaacgc attggcttta accgcagcta ttatctggcc tccttcattt
    32221 ttgccgctgg ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc
    32281 gttttgtcgc gggcgtcggc tgtgccatga tttgggtagt cgttgagagc gcgctgatgt
    32341 gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg
    32401 tgggaacatt tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat
    32461 tgccgtgggt tacaggtttg acgttggcag ggatcttacc gctgttgttt acgcgtgtcc
    32521 tgaatcagca ggctgaaaac catgattcga cgtcaattac gtcaatgcta aaactccgtc
    32581 aggcgcggct tggcgtgaat ggctgcatta tctcaggaat cgtgctggga tctctatatg
    32641 gcctgatgcc gctgtacctc aatcacaaag gggtgagcaa tgccagcatt ggtttctgga
    32701 tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata
    32761 agtttggtcg actgctggtg ttgcgtgttc aggtctttgt cgtcattctc ggcagtatcg
    32821 cgatgcttag ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta
    32881 cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg
    32941 cgatgaacca ggccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta
    33001 ccgctatgct aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat
    33061 cgtttatcta tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc
    33121 acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt
    33181 cttctatctg acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg
    33241 ttataatttt gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt
    33301 tacagatgcc cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcaggaaaa
    33361 acagctgcga tggtacaaca ttgcgctgat gtcttttatc actgtctggg gttttggcaa
    33421 cgttgttaat aactatgcca accaggggct ggtggttgtt ttttcatggg tgtttatctt
    33481 tgcgctctat ttcacacctt atgcactaat tgttggtcag ttaggctcga ccttcaaaga
    33541 tgggaagggc ggggtcagta cctggattaa acacacgatg ggacccggat tggcttacct
    33601 cgccgcgtgg acctactggg tggtgcatat tccctatctg gcacaaaaac cccaggcaat
    33661 actgattgcg ctcggttggg cgatgaaagg cgacggttcg ctaatcaaag aatattcagt
    33721 cgtagcgtta caggggttaa cgctggtgct gtttatcttc tttatgtggg ttgcttcacg
    33781 cggtatgaaa tcgctgaaaa tcgtcggttc tgtggcaggg attgcgatgt ttgttatgtc
    33841 gctcctgtat gtggcgatgg cggtaaccgc gcctgcgatt actgaagtgc atattgcgac
    33901 cacaaacatt acctgggaaa cgttcattcc tcatatcgac tttacctaca ttaccactat
    33961 ttcaatgctg gttttcgcgg ttggcggagc tgagaagatt tctccttacg ttaatcaaac
    34021 gcgcaaccca ggaaaagaat ttccaaaagg gatgttatgc ctggcggtga tggttgcggt
    34081 ttgtgccatt ctgggctcac tggcgatggg gatgatgttt gactcgcgta atatcccgga
    34141 tgacttaatg actaacggtc agtattacgc ctttcagaag ctgggcgagt attacaacat
    34201 gggtaatact ttaatggtga tttacgccat tgcgaatacc ctgggacaag tagctgcgct
    34261 ggtattctcg attgatgccc cgcttaaagt gctattaggc gatgctgaca gcaaatatat
    34321 tccagccagt ttatgtcgta ccaacgcttc tggtacgccc gttaatggct attttctgac
    34381 cctggtgctg gtggcgatcc tgattatgct gccgactctc ggcatcggtg atatgaacaa
    34441 tctctataaa tggctgttga atcttaactc ggtagtgatg ccgctgcgtt atttatgggt
    34501 atttgttgca tttattgcag tcgttcgcct ggcgcagaaa tatacaccag agtatgtctt
    34561 tattcgtaat aagcctctgg caatgaccgt cgggatttgg tgttttgcct ttactgcctt
    34621 tgcctgtttg acggggatct tcccgaaaat ggaagccttc actgcagagt ggaccttcca
    34681 gttggcgctg aatgttgcaa cgccgtttgt gctggtagga ttaggactga tattcccgct
    34741 gctggcgcgt aaagcgaata gtaaataatt attgtggtcg gtgacgctga ctctggcaga
    34801 tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc gcaggacata tactggcggt
    34861 ttgggtaata catttggaag ctgtgctgta agggcgttag aaatagagct ctgaattatt
    34921 tatgcagcgc ggaataaaaa agcggcaact caataaagtt gccgctttac ggggaaatta
    34981 gaacattacc ttatgaccgt actgctcaag aatacctttc acgcgttcca tggtttcttt
    35041 cttcggtggt ttaacaccat cgagtttgta ttcttcaccc atcgccaccc atttgtgttt
    35101 gcccagttcg tggtagggga gaagttcgat tttctcaacg ttacccatat cacgggtaaa
    35161 ttcaccgagg cgatgcgctg aatcgtcatc gtcagaccag cctgggacaa caacgtagcg
    35221 gatccacacc ttcacatttt tattcgccag atatttggcg aactccagcg tgcggtggtt
    35281 ggaaactcca accagatttt ggtggatctc gtcgttcatc tgtttgagat cgagcattac
    35341 caggtcggtt acttccagca gttcatcaat caccggatcg taacgacgaa caaaaccgtt
    35401 ggtgtccaga caggtatgaa tgccttcttt tttgcaggcg cggaaccagt cacgaacaaa
    35461 ctcagcttgc aggattgctt caccgccgga tgcggtaacg ccgccgccgg aagcgttcat
    35521 aaagtggcga taggtcacca cttccttcat caaatcttca acggtaactt ctttaccgcc
    35581 atgcgtgtcc caggtgtcgc ggttatgaca atacaggcag cgcatcaggc agccctggaa
    35641 aaaggtgata aagcgaatac ctgggccgtc tacggttcca caggattcaa aggagtgaat
    35701 gcgaccaata actgacattg cggtgtttct ccagatgtgg cccatctgag gccgtgttgg
    35761 tgcgcagctc gaaggctacg tcgagtctgt tttggcagtc accttaaagt atagatagct
    35821 gacaaaaaag gctcacgtgc taaaaaaggc cccactttcg tggagccttt attgtacgct
    35881 ttttactgta cgatttcagt caaatctaat tacatagatt gagtgaaggt acgagtaata
    35941 acgtcctgct gctgttcttt agtcagcgag ttgaaacgta ctgcgtagcc agatacacgg
    36001 atggtcagct gcggatattt ttccggattt tccatcgcgt cgagcagcat ttcacggttc
    36061 atcacgttaa cgttcaggtg ctgaccacct tcgatggatg cttcgtggtg gaagtaacca
    36121 tccatcagac cagccaggtt ggtcttacga acttcgtcgt ctttacccag tgcgttcgga
    36181 acgatagaga aggtgtagga gataccatct ttagcgtaag caaacggcag tttagcaacg
    36241 gaagtcagag acgctacagc acctttctgg tcacgaccgt gcatcgggtt agcacccggt
    36301 ccgaacggcg cgccagcacg acgaccgtct ggggtgttac ccgttttctt accatacaca
    36361 acgttagaag tgatggtcag aacagactga gtcgggatag cgtcacggta ggtgtgcagt
    36421 ttctgaattt tcttcatgaa acgttctacc aggtcaacag ccaggtcatc tacacgcgga
    36481 tcgttgttac caaactgcgg gtattcgcct tcgatttcga agtcgatagc cagaccgtct
    36541 tcgtcacgaa tcggtttaac tttcgcatat ttgattgcag acagggagtc agcagcaacg
    36601 gacagaccag cgataccaca cgccatggtg cggataacgt cacggtcgtg cagcgccatc
    36661 agagaggctt cgtagctgta cttgtcgtgc atgtagtgaa tgatgttcag tgcagtgatg
    36721 tactgtttag ccagccagtc catgaagtga tccatgcgct ccatcacttc atcgtagttc
    36781 aggagatcgc ctttgatcgg ttcagacttc ggaccaacct gcattttcag tttttcgtca
    36841 acgccgccgt tgattgcgta cagcatggtt ttcgccaggt ttgcacgcgc accgaagaac
    36901 tgcatttgtt taccaacgat catcgggctt acgcagcaag cgatagcgta gtcatcgttg
    36961 ttgaagtccg gacgcatcag gtcatcgttc tcatactgca gagaagaggt gtcgatggac
    37021 actttagcgg cgaatttctt gaagttcagc ggcagttttt cagaccacag aatggtcatg
    37081 ttcggttccg gagacggacc catggtgtac agggtgttca ggaaacggaa gctgtttttg
    37141 gtaaccaggg tacgaccgtc gaggcccata ccaccgatag attcggttgc ccagatcggg
    37201 tcgccagaga acagttcatc gtactccgga gtacgcagga agcgaaccat acgcagtttc
    37261 atgaccaggt ggtcaaccat ttcctgcgct tcttgttcgg tgatcttgcc agctttcagg
    37321 tcacgttcga tgtacacatc caggaaggtg gaggtacgac cgaaggacat tgcagcaccg
    37381 ttctgagact taacagcagc caggtagccg aagtaagtcc actggatagc ttcctgagcg
    37441 ttggtagccg gaccagagat gtcgtagccg tatttcgcag ccatttcttt catctgaccc
    37501 agagcgcggt gctgttcagc gatttcttcg cgcagacgga tagtctgttc caggtttacg
    37561 ccgttttcca gatcagcctg cagagaggtg aactgagcgt atttgtcttt catcaggtag
    37621 tcgataccgt acagcgcaac gcgacggtag tcaccgatga tacggccacg gccataagca
    37681 tctggcagac cggtcagaac accggattta cggcaacgca ggatgtccgg agtgtaaacg
    37741 tcgaacacgc cctggttgtg agttttacgg tattcagtga agattttttt gatcatcggg
    37801 tccagttcgc ggttgtacgc tttgcaggaa ccttcgatca ttttgatacc accgaacggg
    37861 ataagagcac gtttcagcgg agcttcagtc tgcagaccaa caactttttc caacgctttg
    37921 ttgatgtagc cagcgtcgtg agaggtgatg gtggaagcaa cagcggtgtc gaagtcaact
    37981 ggcgcgtgag tgcggttttc cagtttaacg ccttccatta ctttgtccca cagggtggtg
    38041 gtcgcttcag tagcgccagc caggaaggac tcgtcaccct cgtacggagt gtagtttttc
    38101 tgaatgaagt cacggacgtt tacttcattc tgccagtcac ctttggtaaa accttcccag
    38161 gctgtggcta acttttcatt aagctcggac atgtaacacc taccttctta agtggatttt
    38221 ttatttactg cgtacttcga caaccattaa tggtgatcgt tttcacgcag gtaaatgacc
    38281 cagtatgtca acccaaccaa caaaccaccg ccgataatat taccgatcgt aaccggaatc
    38341 aggttatcag tgatgaagtt catcacggtc aggtgagaaa aattttccgg tgcagaaccg
    38401 acagcggtcc agaattccgg agatgcgaag tcgcggatta caatacccat cgggatcata
    38461 aacatgtttg cgatactgtg ctcaaaaccg ctggcaacaa acatcgcgac cggcagcacc
    38521 atgataaacg ctttgtccat caggctgcgg ccagaataac tcatccatac tgccagacat
    38581 accatcaggt ttgccaggat accaaggcag acggcctcaa taaaagtatg gtgcactttg
    38641 tggtcggcgg tttgtaggac gtttagtccc cattgaccat ttgcggtcat atactcgccg
    38701 gaaagccaca ttaaaagtac aaacagcagt gcgccgacca ggttgccaaa atagacattt
    38761 agccagtttt tagccaactg accccaggtg atgcgcccac tcgccttagc aacaacaatc
    38821 aacactgtgg aagtaaagag atcggctccg cagacaacac aaagaatcag tcccagagag
    38881 aagcaaatgc cgccaaccag ttttgccatg ccgaagggca ttgtgcctgt gccagtggtt
    38941 gctgtgatat agaagacgaa tgcgattgag atgaaaacac cggcggtaat cgccagatag
    39001 aaagtcttaa gcggatgttt cgttgcttta tagacacccg cctcttcggc cactttggcc
    39061 attgcagcag gaagtaaaag atcaaaaggg ttgtcagct
//
LOCUS       DBFIFS010000022        38474 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.022, whole genome shotgun sequence.
ACCESSION   DBFIFS010000022 DBFIFS010000000
VERSION     DBFIFS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.022"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            3..125
                     /locus_tag="N7A07_001026"
     CDS             3..125
                     /locus_tag="N7A07_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU2475740.1"
                     /translation="MGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            104..208
                     /gene="speFL"
                     /locus_tag="N7A07_001027"
     CDS             104..208
                     /gene="speFL"
                     /locus_tag="N7A07_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU2475741.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            519..2717
                     /gene="speF"
                     /locus_tag="N7A07_001028"
     CDS             519..2717
                     /gene="speF"
                     /locus_tag="N7A07_001028"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU2475742.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2714..4033
                     /gene="potE"
                     /locus_tag="N7A07_001029"
     CDS             2714..4033
                     /gene="potE"
                     /locus_tag="N7A07_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU2475743.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4090..5730)
                     /gene="pgm"
                     /locus_tag="N7A07_001030"
     CDS             complement(4090..5730)
                     /gene="pgm"
                     /locus_tag="N7A07_001030"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU2475744.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5756..6301)
                     /gene="seqA"
                     /locus_tag="N7A07_001031"
     CDS             complement(5756..6301)
                     /gene="seqA"
                     /locus_tag="N7A07_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU2475745.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6486..7250
                     /gene="ybfF"
                     /locus_tag="N7A07_001032"
     CDS             6486..7250
                     /gene="ybfF"
                     /locus_tag="N7A07_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2475746.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7390..7683
                     /gene="ybfE"
                     /locus_tag="N7A07_001033"
     CDS             7390..7683
                     /gene="ybfE"
                     /locus_tag="N7A07_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU2475747.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7823..8353
                     /gene="fldA"
                     /locus_tag="N7A07_001034"
     CDS             7823..8353
                     /gene="fldA"
                     /locus_tag="N7A07_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU2475748.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8563..8649
                     /gene="uof"
                     /locus_tag="N7A07_001035"
     CDS             8563..8649
                     /gene="uof"
                     /locus_tag="N7A07_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU2475749.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8642..9088
                     /gene="fur"
                     /locus_tag="N7A07_001036"
     CDS             8642..9088
                     /gene="fur"
                     /locus_tag="N7A07_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU2475750.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9172..9498)
                     /gene="chiQ"
                     /locus_tag="N7A07_001037"
     CDS             complement(9172..9498)
                     /gene="chiQ"
                     /locus_tag="N7A07_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU2475751.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9548..10953)
                     /gene="chiP"
                     /locus_tag="N7A07_001038"
                     /pseudo
     CDS             complement(9548..10953)
                     /gene="chiP"
                     /locus_tag="N7A07_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11532..13196)
                     /gene="glnS"
                     /locus_tag="N7A07_001039"
     CDS             complement(11532..13196)
                     /gene="glnS"
                     /locus_tag="N7A07_001039"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU2475752.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13399..15345)
                     /gene="nagE"
                     /locus_tag="N7A07_001040"
     CDS             complement(13399..15345)
                     /gene="nagE"
                     /locus_tag="N7A07_001040"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU2475753.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15678..16478
                     /gene="nagB"
                     /locus_tag="N7A07_001041"
     CDS             15678..16478
                     /gene="nagB"
                     /locus_tag="N7A07_001041"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU2475754.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16538..17686
                     /gene="nagA"
                     /locus_tag="N7A07_001042"
     CDS             16538..17686
                     /gene="nagA"
                     /locus_tag="N7A07_001042"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU2475755.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17695..18915
                     /gene="nagC"
                     /locus_tag="N7A07_001043"
                     /gene_synonym="nagR"
     CDS             17695..18915
                     /gene="nagC"
                     /locus_tag="N7A07_001043"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU2475756.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18963..19715
                     /gene="nagD"
                     /locus_tag="N7A07_001044"
     CDS             18963..19715
                     /gene="nagD"
                     /locus_tag="N7A07_001044"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU2475757.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19972..21636
                     /gene="asnB"
                     /locus_tag="N7A07_001045"
     CDS             19972..21636
                     /gene="asnB"
                     /locus_tag="N7A07_001045"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU2475758.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22016..22092
                     /locus_tag="N7A07_001046"
     tRNA            22016..22092
                     /locus_tag="N7A07_001046"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22050..22052,aa:Met,seq:cat)
     gene            22103..22187
                     /locus_tag="N7A07_001047"
     tRNA            22103..22187
                     /locus_tag="N7A07_001047"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22137..22139,aa:Leu,seq:tag)
     gene            22211..22285
                     /locus_tag="N7A07_001048"
     tRNA            22211..22285
                     /locus_tag="N7A07_001048"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22243..22245,aa:Gln,seq:ttg)
     gene            22320..22394
                     /locus_tag="N7A07_001049"
     tRNA            22320..22394
                     /locus_tag="N7A07_001049"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22352..22354,aa:Gln,seq:ttg)
     gene            22410..22486
                     /locus_tag="N7A07_001050"
     tRNA            22410..22486
                     /locus_tag="N7A07_001050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22444..22446,aa:Met,seq:cat)
     gene            22535..22609
                     /locus_tag="N7A07_001051"
     tRNA            22535..22609
                     /locus_tag="N7A07_001051"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22567..22569,aa:Gln,seq:ctg)
     gene            22647..22721
                     /locus_tag="N7A07_001052"
     tRNA            22647..22721
                     /locus_tag="N7A07_001052"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22679..22681,aa:Gln,seq:ctg)
     gene            complement(22875..24050)
                     /gene="ubiF"
                     /locus_tag="N7A07_001053"
     CDS             complement(22875..24050)
                     /gene="ubiF"
                     /locus_tag="N7A07_001053"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU2475759.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            24196..25620
                     /gene="miaB"
                     /locus_tag="N7A07_001054"
     CDS             24196..25620
                     /gene="miaB"
                     /locus_tag="N7A07_001054"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU2475760.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25773..26813
                     /gene="ybeZ"
                     /locus_tag="N7A07_001055"
     CDS             25773..26813
                     /gene="ybeZ"
                     /locus_tag="N7A07_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU2475761.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26810..27277
                     /gene="ybeY"
                     /locus_tag="N7A07_001056"
     CDS             26810..27277
                     /gene="ybeY"
                     /locus_tag="N7A07_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU2475762.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27367..28245
                     /gene="corC"
                     /locus_tag="N7A07_001057"
     CDS             27367..28245
                     /gene="corC"
                     /locus_tag="N7A07_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU2475763.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28270..29808
                     /gene="lnt"
                     /locus_tag="N7A07_001058"
     CDS             28270..29808
                     /gene="lnt"
                     /locus_tag="N7A07_001058"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU2475764.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            30205..31113
                     /gene="gltI"
                     /locus_tag="N7A07_001059"
     CDS             30205..31113
                     /gene="gltI"
                     /locus_tag="N7A07_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU2475765.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAIVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31283..32023
                     /gene="gltJ"
                     /locus_tag="N7A07_001060"
     CDS             31283..32023
                     /gene="gltJ"
                     /locus_tag="N7A07_001060"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU2475766.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            32023..32697
                     /gene="gltK"
                     /locus_tag="N7A07_001061"
     CDS             32023..32697
                     /gene="gltK"
                     /locus_tag="N7A07_001061"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU2475767.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32697..33422
                     /gene="gltL"
                     /locus_tag="N7A07_001062"
     CDS             32697..33422
                     /gene="gltL"
                     /locus_tag="N7A07_001062"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU2475768.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33540..34475
                     /gene="rihA"
                     /locus_tag="N7A07_001063"
     CDS             33540..34475
                     /gene="rihA"
                     /locus_tag="N7A07_001063"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU2475769.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            34559..36229
                     /gene="hscC"
                     /locus_tag="N7A07_001064"
     CDS             34559..36229
                     /gene="hscC"
                     /locus_tag="N7A07_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU2475770.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(36289..37740)
                     /gene="djlC"
                     /locus_tag="N7A07_001065"
                     /pseudo
     CDS             complement(36289..37740)
                     /gene="djlC"
                     /locus_tag="N7A07_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(37737..38444)
                     /gene="ybeU"
                     /locus_tag="N7A07_001066"
     CDS             complement(37737..38444)
                     /gene="ybeU"
                     /locus_tag="N7A07_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2475771.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9426 a         9832 c         9714 g         9502 t
ORIGIN      
        1 atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata
       61 gaataactct accttcaact gacacagcaa gaggtaaagg taaatggaaa ataataaccg
      121 cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt
      181 tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc agcgtacagg
      241 tctccctgac tttaagcatt acaggttaat acctgtatcc ctcggtgctc atatactgct
      301 aaccctttta aactctacat aattcgagtc gcagcatatg caacttgaag tatgacgagt
      361 atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac tactgattta
      421 tttatcagcg gtggagcttt gctttttttc cggcgtgatc gatttctcct ttgagaaatt
      481 gaggacctgc tattacctga aataaagaga tgaacaaaat gtcagaatta aaaattgccg
      541 ttagtcgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat atagacgaaa
      601 gtaattatat tgacgttgcc gccattattt tatcagtcag tgatgttgaa cgtggaaaac
      661 tcgatgaaat agacgctact ggctatgaca ttcctgtttt tattgcaacg gaaaatgaag
      721 aacgtatacc agcagaatat ctttcacgta tttctggtgt cttcgaacat ggagaagcac
      781 gcaaagaatt ttatggtcgt cagttagaaa ccgctgccag ccattatgaa actcaactgc
      841 gcccaccttt ctttcgcgca ctggtcgatt atgtcaatca gggcaacagc gcatttgact
      901 gccccggtca tcagggcggc gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg
      961 aatactttgg tgaggcgctg ttccgtgccg acttgtgcaa cgccgacgta gcgatgggcg
     1021 atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcagca aaagtgttta
     1081 atgccgataa aacctacttc gttttaaatg gcacttcatc ttctaacaaa gtggttttaa
     1141 acgccctgct gacaccgggt gatctggtgc tatttgaccg caataaccat aagtctaacc
     1201 accacggggc cttgctacag gctggtgcaa caccggttta tctggaaaca gcacgtaacc
     1261 cgtatggttt tatcggtggt attgatgcac actgcttcga agaaagttat ctgcgtgaac
     1321 tgattacaga agttgcaccg caacgggcaa aagaggcgcg tccgttccgc ctcgctgtga
     1381 ttcagctagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg gataaaattg
     1441 gtcatctgtg tgactacatc ctgtttgatt ccgcatgggt cggctatgaa cagtttattc
     1501 cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc
     1561 tggttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata
     1621 aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg
     1681 cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca
     1741 acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg
     1801 gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcatacctg
     1861 aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc
     1921 gcttcttcca gtttgtacca ggtgagcact ggcattcttt tgaaggctat gcagagaatc
     1981 aatactttgt tgatccgtgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg
     2041 gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg
     2101 gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag
     2161 atatggccaa acttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgctggaat
     2221 ctgatgcgcc gctggcggaa gtgctacctt ccatctacaa acagcatgaa gagcgctacg
     2281 ccggttatac ccttcgtcag ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg
     2341 tgaaacaact gcaaaaagag atgttccgta aggaacactt cccacgcgtc agcatgaatc
     2401 cgcaagaagc taactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg
     2461 cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg
     2521 ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg gaagaaggga
     2581 tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg
     2641 gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgctga
     2701 aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat
     2761 actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga
     2821 agttgggaca atctcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc
     2881 atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg gcggttatgc
     2941 cgagtatgct ttcggtaaat ccggtaactt tatggcgaac tatacctacg gcgtctcact
     3001 gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg
     3061 cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac
     3121 cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta ctgtgtgggg
     3181 agtcattatt ccggtcgtcg gtctgtgcat tattggctgg ttctggttta gcccgacgct
     3241 gtacgttgat tcctggaatc cgcatcatgc accgttcttc agtgcggtag gttcttccat
     3301 cgctatgacg ctgtgggctt ttcttggtct ggagtctgcg tgtgcaaata ctgatgtagt
     3361 ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt
     3421 gatttatatc gtctccacca acgtgattgc cgggattgtg ccaaatatgg agctggcaaa
     3481 ttcaacggca ccatttggtc tggccttcgc gcagatgttc acgccggaag tgggtaaagt
     3541 cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat
     3601 tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt tctcccatgt
     3661 aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt
     3721 ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc
     3781 cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa
     3841 ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc
     3901 gatgtacagc ttttatgcgc tgtactcatc cggggaagaa gccatgctgt acggttccat
     3961 cgtgaccttc cttggttgga cactgtatgg tctggtatca ccacgctttg aactgaaaaa
     4021 taaacacggt taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc tttttcttat
     4081 ttaatgtgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat
     4141 ctgcttgcga tgttcttcac cgaggaagct ttcgcagtag atcttatatg cgtcttccgt
     4201 gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccaataga
     4261 agcaccgttg cccggagcag cagtcaggcg cgcggtgatc gggtcaccag ccagggtgct
     4321 ggcactcacc atttccggag acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc
     4381 tgcctgcaaa cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc
     4441 ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca tgatgatgcc
     4501 gtctttgtcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc
     4561 ttcgccgcca aagccgaagc tgccgtcaaa cagaccatca acaaaccatt tgaaacctac
     4621 cgggacttct accagtttac ggcccaagtc gttgaccaca cggtcgatca tcgcagatga
     4681 aaccagcgtt ttaccgacgg caacagcttt gccccactgc ggacgatgct ggaacaggta
     4741 gttgattgcc accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc
     4801 gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag
     4861 caggcccgcc atcgcacact cggaggagca gtccatacgg atcgcgccgt ctttatcaag
     4921 gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta
     4981 atactcgcca atacgcttcc agtattcgat accggaaccg cccagcggat caacgccaag
     5041 cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacaaa
     5101 cggctgcacc agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat
     5161 acgcttcacg cctttcaggc catcggccat cagtgcgttg gctctgtctt ccaccacttt
     5221 agtgacgttg gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc
     5281 cggcgggtta tgggacggtg taataacgat accgtctgcc agcgggccac cttttttatt
     5341 gtgaaccagg atggcattgg aaacggcagg cgtcggggtg aagccattgt tttcctgcac
     5401 aataacatct acgccgttcg ctgccagcac ttccagaacg gaaataaatg caggttcgga
     5461 cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc
     5521 ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc
     5581 actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg
     5641 tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc
     5701 aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga
     5761 tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt
     5821 cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg
     5881 gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa
     5941 agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa
     6001 gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcat
     6061 attcatccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgatcg
     6121 gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag
     6181 gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga
     6241 tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca
     6301 tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc
     6361 cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg
     6421 cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact
     6481 ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca
     6541 tcgttcttgt ccatggtctg tttggcagcc tcgacaacct tggcgtactg gctcgcgatc
     6601 tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag
     6661 atccggtaat gaattacccg gcgatggcgc aggatcttgt tgatactctg gatgcacagc
     6721 agatcgacaa agcaacattt atcgctcact ccatgggcgg taaagcggta atggcactta
     6781 ctgcactggc ccccgatcgc atcgataaac tggtggcgat cgatatcgca ccggtcgact
     6841 atcacgtacg ccgtcatgat gagattttct cggctatcaa cgcggtcagt gaatcggacg
     6901 cacaaactcg ccagcaagca gcagcaataa tgcgccagca tcttaatgaa gaaggggtga
     6961 ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccggtattgt
     7021 gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc
     7081 tgtttatccc tggtggtaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg
     7141 cacaatttcc acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg
     7201 aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat
     7261 taaaaaccca gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg
     7321 atgtattatg gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc
     7381 accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag
     7441 cgtcgaccgg gacgaccgaa aacaaatccg ctttcgcgcg atgaacagct gcgtattaat
     7501 aaacgcaacc aactaaaacg cgacaaagta cgtggcctaa agcgtgtcga actgaagctg
     7561 aacgcggaag ctgtcgaggc gctgaacgag ctggcggaat cgcgcaatat gagccgtagc
     7621 gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt
     7681 taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga
     7741 ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa
     7801 gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg
     7861 taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt
     7921 ccatgacatt gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg
     7981 catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct
     8041 cgaagagatt gatttcaatg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga
     8101 ttacgccgaa tatttctgcg acgcattggg caccatccgc gacatcattg aaccgcgcgg
     8161 tgcaaccatc gttggtcact ggccaactgc gggctatcat ttcgaagcat caaaaggtct
     8221 ggcagatgac gaccactttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac
     8281 cgctgaacgt gtagaaaaat gggttaaaca gatttctgaa gagttgcatc tcgacgaaat
     8341 tctcaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc
     8401 caatcaaaat aattgctaca aatttgtaac ttttgccgtt gtacctgtac aatgtcccgg
     8461 tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt
     8521 gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca
     8581 agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg
     8641 catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt
     8701 aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata
     8761 caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa
     8821 ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt
     8881 tgaactgaca cagcaacatc accacgatca cctgatctgc ctcgactgcg gcaaggttat
     8941 cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat
     9001 tcgcctgact aaccacagtc tctatcttta cggtcactgc gccgaaggcg attgccgcga
     9061 agatgagcac gcgcacgaag gcaaataagc cagtctgaac gagaaaagcc aacctgcggg
     9121 ttggcttttt tatgcaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt
     9181 attactgcga atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc
     9241 ggtttgcgtt gcacgcagac actgctgata atccagtacg cggacacttt cctggtcggc
     9301 aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat
     9361 tttttccggc gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacggct
     9421 gtcttcctgt ggcgcggttg attgcgcaca agctaccagc cccgatgcca tcatggcgat
     9481 gagaatcagt tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg
     9541 tcgggcatca gaagatggtg aatggtgcga ttaccataaa ttttacgtca cgctcatcct
     9601 ggaagatgtt gccgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
     9661 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
     9721 ccaggctgta tgcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
     9781 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
     9841 ctggaagatt ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt
     9901 tggcgttgaa gtcagaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt
     9961 aggttggagt catacgttgc aggaagtatc cctgctgacc gtcagcttta acccaggtgc
    10021 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc
    10081 aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccgt
    10141 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    10201 caaagtattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat
    10261 ttttgaagtc gtatttcgcc ccaagggagt gcagataatc aactttggtg gttttatcgt
    10321 tctgataaaa ctcatccatt tccagatgcc acggtgcttt gtattcgttg gtccacatgt
    10381 aggagaaact caacgcacca gcatcgccgt aatcaaaatt cgccccggcc tccgcaccct
    10441 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtttgacca gttggctgaa
    10501 tataacctgc cctcgccaca ccggaccgta tttaaatttg gccgctgctt tatacaggct
    10561 tataccgctt ttatcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc
    10621 gatttcgttc gggtgagagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcggc
    10681 aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt tggcgttcca
    10741 ggtggagtga gaaaggttgg ttttgtattt atcgccgtcg gtaacatctt tacggtcgcg
    10801 ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcaatga atccttcggc
    10861 gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga tagccagcgc
    10921 cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt
    10981 gctgcaccac aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    11041 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    11101 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    11161 gtttgcggtt gaaaggcttg cagaagaata aaagcgtgaa attccagcaa gatgaaagcg
    11221 gttacatttt atctaattat gttttgtgtt tttaaattta ttttatggat aaattgcgaa
    11281 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgct gaacgcggcg ttttggattg
    11341 tttgcatatt tcttgccgga tgcggcgtga acgccttatc cggcctacaa aagcacgcaa
    11401 actcaatata ttgcagagat catgtaggcc tgataagcgt agcgcatcag gcaatttagc
    11461 gtttgaaact gtttgtctgt tccttgccgg atgcgacgta aacgccccat ccggcatagc
    11521 gaaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta
    11581 aataccggtt tttccgccgt agaatggcgg ctatcgaggc agaagtaacc ttcacgctca
    11641 aactggaatg ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg
    11701 atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc tgggttcggc
    11761 acgctgaaca gacggtcata caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc
    11821 cagtgaataa cacctttgac tttacgacca tctgccggat ctttgcttaa ggtatcggcg
    11881 tcataagtac agaagatggt ggtgatatta ccttcggcat ctttctcgac gcgttcagcc
    11941 ttaatcacat aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc
    12001 ttgttagctt cttcgcggaa atcggagcga tcaatccaaa tctcaccgct aaacggcacc
    12061 tgacggctcc ccatttccgg tttgttcgga tggttcggca tggtaaccat ttcgccttcg
    12121 ccctgatagt tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca
    12181 ttttcgttga gatcttcacg gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc
    12241 tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagccgcagt gtaaccacga
    12301 cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg
    12361 gtcaccagca agttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag
    12421 aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt ccagtaccca gtcgtacaga
    12481 cgacggttat cctggaactc aagcgtacac agagagtgcg taataccttc cagggcatcg
    12541 ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg
    12601 tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc
    12661 atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccgccggt acgcattttc
    12721 tcgaacagcg ccaggttctc ttcaacgcta cggtcgcggt acgggctgtt tttaccaggc
    12781 tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc
    12841 aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag
    12901 taacggacgt taccagacca gtgaaaacct aaccactcaa cgtcgttttt gatcgaatcg
    12961 acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct
    13021 ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga
    13081 tagccattcg gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga
    13141 tcttcatcga tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg
    13201 gattcctcaa agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga
    13261 caactgttag ttagtgaaaa tcgaaaatct cactgagatt atggggatga ttgatgaaaa
    13321 aaaaagcggc agagaagatc tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg
    13381 catcaggcat aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac
    13441 atggccctga gctttaatga tcaagccact gaaatcgtcg atattgctgc aaaccaccgg
    13501 gctaatcatc gaacgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg
    13561 ccctgcgctt acctgcgcgc cctcttccac cagacgttta aagcctttac cttccagcgc
    13621 tacggtatcg atacccatgt gaacgacaat ctccgcgcct ttttcggttt ccaggcagaa
    13681 cgcgtggttg gtgttgaaga ttttcacgat tgttcctgcg gctggtgata cgacgatttt
    13741 atctgtcggt ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg
    13801 aacctgatcc agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac
    13861 cgcgtttggt acagcctgcg gttttgctac aggagcggca gttgccggag ttgcttcagc
    13921 agaggcagct gctaccgggc cacgggcaac gactttcttc atcgcatcgc cgatggattc
    13981 tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc agttttacta ccccagaagc
    14041 ccccagacgt ttacacatcg tatcgttaac gcgggcagag tcagccacgg taaggcgcag
    14101 acgggtgata caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg cagcaatata
    14161 gttggtcgcc agttgattga ggccttcttc agtgttgctg ttggcttctt cagtaacgat
    14221 ctcgtcttct ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact
    14281 gaacaccacg aagtagatag cgaagaagac aacacccata accagcagca tccagacgtt
    14341 ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg atagcccccg cagagaaaga
    14401 gaagccggca tggataccca gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc
    14461 gtgcaggagg tacagcagcg gagcaaggaa catgaacagg aattccagcg gctcagtcac
    14521 accggtcagg aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg
    14581 ctctttcggt gctgcgaagt acatcgccag cgccgcaccc ggcaggccga acatcatgat
    14641 cgggaagaag ccggacatga acatccccgc ggtgccgtca ccagcgtaga aacggttgat
    14701 gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat
    14761 ggtgttcagc acctgatgca gaccggttgg gatcaacaga cggttgatga aaccaaagat
    14821 accggaaccc agcgcgcccg cagaaacgat ccactcgccg cctgcatgga tagcgtgctg
    14881 taccggcggc cagacgtaac caaaaatggc cgccagcacc agacagaaga agccggtggc
    14941 gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt taatatcgga
    15001 ccaacggtta taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat
    15061 gttaatttct gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcccc
    15121 cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa
    15181 gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg caacgttaag
    15241 taaatctggc tgaccgaatc gcagcaacag tgccgccacc ggcagcaccg cgatagggag
    15301 ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg
    15361 agaaccctat ttggctcgtt taaagccgta tttttattgt gctgcaaatt gtactgccga
    15421 tgttctgtaa tcagattgtt agatcatctg ccacagagtg tgtgaaaatt taattcgtat
    15481 cgcaaattaa acgagtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc
    15541 ttttactggc taaaccagaa aacttatttt atcattcaaa aaatcaggtc ggattgacgc
    15601 ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg cttacatttt
    15661 acttattgag gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat
    15721 gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa accgactgct gatcgtccgt
    15781 ttgttctggg cctgccaact ggcggcacgc caatgaccac ctataaagcg ttagtcgaaa
    15841 tgcataaagc aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg
    15901 tcggtctgcc gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg
    15961 atcacgttga tattccagca gaaaacatca accttctcaa cggcaacgcc ccggatatcg
    16021 acgccgagtg ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta
    16081 tgggcggtgt aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt
    16141 ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt cgcgaactct cgtttctttg
    16201 ataacgatgt caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acactgctgg
    16261 atgccgaaga agtgatgatt ctggtgctgg gtagccagaa agcactggcg ctgcaggccg
    16321 cagttgaagg ttgcgtgaac catatgtgga ccatcagctg tctgcaactg catccgaaag
    16381 ccatcatggt gtgcgatgag ccttccacca tggagctgaa agttaagact ttaagatatt
    16441 tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggctcc
    16501 ttgctcaggg caatattttt taaaatcggg ggtcagaatg tatgcattaa cccagggccg
    16561 gatctttacc ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat
    16621 taaaagcgtc tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg
    16681 ggccattctc tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt
    16741 taacgacacc gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga
    16801 gaaatcaggc tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa
    16861 acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct
    16921 gcatctggaa ggcccgtggc tgaatctggt gaaaaaaggt acccataatc cgaattttgt
    16981 gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa
    17041 agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg
    17101 gattgtggtt tctgccggtc actccaacgc gacgttgaaa gaagcgaaag ccggtttccg
    17161 cgcggggatt acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga
    17221 accgggcctg gcgggcgcga tcctcgatga agctgacatt tattgcggta ttatcgctga
    17281 tggcctgcat gttgattacg ccaacattcg taatgctaaa cgccttaaag gcgacaaact
    17341 gtgtctggtt accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc
    17401 gggtaaaaca atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg
    17461 ttcatcctta accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact
    17521 ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg
    17581 tctcggcaca ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa
    17641 aatcaccaag accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca
    17701 ccaggcggac aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg
    17761 gtttatcgcc tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc
    17821 cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc
    17881 aaagaagttg atcagcaggc ctccaccggg ggccgccgcg ctatctctat cgtcaccgaa
    17941 acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc acgacgccac catcaccctg
    18001 tttgatctca gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag
    18061 cagacgctgg aacatgccct gctgaatgcc attgctcagt ttattgatag ctaccagcgc
    18121 aaactgcgcg agctgatcgc catttccgtt atcctgccag gacttgttga cccggacagc
    18181 ggcaaaattc attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg
    18241 gaagagcgtt ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg
    18301 gagcactact tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc
    18361 ggaaccgggg ccgggattat ctctaacggg cgcattttta ttggccgcaa cggcaacgtc
    18421 ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt
    18481 ggctgcctgg aaactatcgc tgccaacgcc gctattgaac aacgggtgtt gaatctgtta
    18541 aagcagggct accagagccg cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa
    18601 gccgcgaaca aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg
    18661 ggcaaaacca tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgccggt
    18721 gaaatcaccg aagccgataa agtgctgctc cctgctattg aaagctgcat taatactcag
    18781 gcgctgaagg cgtttcgtac taatctgccg gtggtacgtt ctgagctaga ccaccgctcg
    18841 gcaatcggtg cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat
    18901 ttgctggaaa attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt
    18961 ccatgaccat taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg
    19021 ccgtaccggg tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt
    19081 tgctgaccaa ctatccttcg cagaccgggc aagatctggc gaaccgcttt gccactgccg
    19141 gtgtcgatgt acctgacagc gtgttttata cctctgcgat ggcgaccgcc gatttcctgc
    19201 gtcgccagga aggcaagaaa gcgtatgttg tgggcgaagg cgcactgatt catgaactgt
    19261 acaaagccgg tttcactatt accgatgtga accctgattt tgtgattgtt ggcgaaacgc
    19321 gttcctacaa ctgggacatg atgcataaag cggcctattt cgtcgctaac ggtgcacgct
    19381 ttatcgccac caacccggac acccacgggc gcggttttta tccggcctgt ggcgcgttgt
    19441 gtgcagggat tgagaaaatc tccgggcgca aaccgttcta tgttggtaag ccgagtccgt
    19501 ggattatccg cgcggcatta aacaaaatgc aggcacattc ggaagaaacg gtgattgtcg
    19561 gcgataacct gcgtaccgat attctggccg gcttccaggc aggtctggag acgattctgg
    19621 tgctttctgg tgtttcgtcg ctcgacgata tcgacagtat gcctttccgc cccagctgga
    19681 tttacccgtc agtcgctgaa atcgacgtta tctgaatagc aaccacgtct caggacgtgg
    19741 tttcttattt ttacccatca ttaaaatcat cccattcaat aaaacccgcc aaaaattatc
    19801 gatccatcaa taaatacatc accgcaccct cccctttttc acaaatcagc aatcaccatt
    19861 acgtttttta ttttttcacc gccaaatcgc ttgcgcacag tgctgctttg cggcattttt
    19921 ttaaacaagc aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca
    19981 atttttggcg tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag
    20041 ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataaa
    20101 gccattctcg ctcacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc
    20161 tacaaccaac aaaaaaccca cgtactggcg gtaaacggtg aaatctacaa tcaccaggca
    20221 ttgcgcgctg aatatggcga tcgttatcag ttccagacag ggtctgactg tgaagtgatc
    20281 ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc
    20341 tttgccctgt acgacagcga aaaagatgcc tacctgattg gtcgcgacca tctggggatc
    20401 atcccactgt atatgggcta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa
    20461 gccctggtgc cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagt
    20521 caggacggcg aaattcgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa
    20581 gataacgtga ccgacaaaaa cgagctgcgt caggctctgg aagattccgt taaaagccat
    20641 ctgatgtctg atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt
    20701 tccgctatca ccaagaaata cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc
    20761 tggtggccgc agttacactc ctttgctgta ggtctgccgg gttcaccgga tctgaaagca
    20821 gcccaggaag tggcaaacca tctgggcact gtgcatcatg aaattcactt cactgtacag
    20881 gaaggtctgg acgccatccg cgacgtgatt taccacatcg aaacctatga tgtgaccact
    20941 attcgcgctt caacaccgat gtatttaatg tcgcgtaaga tcaaagcgat gggcattaaa
    21001 atggtgctgt ccggtgaagg ttctgatgaa gtgttcggcg gttatcttta cttccacaaa
    21061 gcaccgaatg ccaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg
    21121 tatgactgcg cgcgtgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg
    21181 ttcctcgaca agaaattcct cgacgtggcg atgcgcatta acccgcagga taaaatgtgc
    21241 ggtaacggca agatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcccgca
    21301 agcgtggcct ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac
    21361 accctgaaag aagtggcggc gcagcaggtt tctgatcaac agctggaaac tgcccgcttc
    21421 cgcttcccgt acaacacacc gacctcaaaa gaagcgtatc tgtaccggga gatctttgaa
    21481 gaactattcc cgcttccgag cgccgctgag tgcgtgccgg gcggtccttc cgtcgcgtgt
    21541 tcttccgcta aagcgatcga atgggatgaa gcgttcaaga aaatggacga tccgtctggt
    21601 cgcgcggttg gtgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa
    21661 atccggggct gtttcagtta ttgaggtgct tgttgattct gatatgcagt aaggaaatgt
    21721 tccgggtttg ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt
    21781 aatttcccac cactattgtt aaattccgaa tttcatcctg tatgacaaaa gaatggctgt
    21841 agggtgggaa aaaacacaaa gtaaacaata attgacgaat atagcgccac gctgttcgca
    21901 acctaaccaa acagtcactt tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg
    21961 ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat cgcgaaaaaa aagatggcta
    22021 cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg
    22081 tcgtagccac catctttttt ttgcgggagt ggcgaaattg gtagacgcac cagatttagg
    22141 ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc accagaaagc
    22201 gttgtacgga tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct
    22261 ggttcgaatc caggtacccc agccatcttc tttgagtaag cggttcaccg cccggttatt
    22321 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    22381 aggtacccca gccatcgaag aaacaatctg gctacgtagc tcagttggtt agagcacatc
    22441 actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt
    22501 cgaatacgtt cggcaaattc aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac
    22561 cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccaa tttattcaag
    22621 acgcttacct tgtaagtgca cccagttggg gtatcgccaa gcggtaaggc accggattct
    22681 gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa agctcgcttc
    22741 ggcgagcttt ttgcttttct gcgttcactc aatgtcgaat gcgatgttga cacgtcttat
    22801 ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggttgg cacgcatccg
    22861 gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt caacacgcca
    22921 gcacgctccg ccgccatcaa accaagatta cgcacaaaac gcagcggcgg cagattatta
    22981 ctgaatccgg cataaaacag atccataccg ctctgcataa tgaagttatc cgccatgcgc
    23041 cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcgccgta gctgcgggca
    23101 ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac cccctgcccc
    23161 gccagcggat ggatggtgtg cgcggcatca cccaccagcg caagccccgg ctgcacatac
    23221 tgcaacgcat ggcggcgtgt cagcggaaac gcaccagcgg caagcggtgt aacgtaaccc
    23281 agacgcgacg ggaaatgctt cgcgatttcc acctgtaact gcgccatatt catattctgc
    23341 aactggcgaa tgcgcgccgg agagtcgtac cacaccagcg atgcccagtt atcaaacagc
    23401 ggcagaaacg cgcgcggtcc atccggggta aattgctgcc aggtgctatc gccgggatcg
    23461 ttctcgcact ggacgctaat caacatgcac gactgcgcat actgccatgc atgaacgcca
    23521 attcccgcca tctgccgcac ctgcgaattt gcgccgtcgg caccaatcac cagcttcgcg
    23581 cgaatcactt caccgccttt cagctccagc tcctgaagat cattatctcg atgtaatgca
    23641 atcagcgagg ttggcacacg taacgttact ttcggatgcg cttccagcgc ctgccacaat
    23701 gcctgttgca ggacagtgtt ttctaccata tagccaagca acggcaactt taattcggct
    23761 gcgtcaaata ccacatgcgc cgtttcccac tcccacgttt ccagtctgcg gtaaggatgg
    23821 caacgcatag cctgtactgc atcccagacc cctaaccctt taagcaatga taccgaagcc
    23881 gcgctgatcg ccgagatccg cacgtccggt tggctatcag cgacaaacgg tgcaggttct
    23941 gcgtgctcga tcaccgttac cgtaaatccg tgctgtgcca gccccagcgc cagtgcgccg
    24001 ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat ggtcaatcat
    24061 cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg ataacgctca
    24121 tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg gctactattt
    24181 cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg tcagatgaac
    24241 gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta tcaactgacc
    24301 gacgtggcgg aagaagcgga tgtgctgctg ctgaacacct gctcaatccg cgagaaggct
    24361 caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa gaatccagac
    24421 ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc
    24481 gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc ggagatgatc
    24541 aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga aatcgagaag
    24601 tttgaccgtc taccggaacc gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa
    24661 ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga agaggtaagc
    24721 cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg cgtgcgtgaa
    24781 gtcaacctgc tcggtcagaa cgtgaacgcc tggcgtggtg aaaactacga cggcaccacc
    24841 ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga tcgtattcgc
    24901 tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta tcgcgacacg
    24961 ccggagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg cattctgaac
    25021 ctgatgggac gtacccatac ggcgctggag tacaaagcga tcatccgtaa actgcgtgcg
    25081 gcgcgtccag atattcagat cagttctgac ttcatcgtcg gcttccctgg cgaaaccacc
    25141 gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat gagctacagc
    25201 tttatcttct ctgcacgtcc gggtacacca gccgccgata tggttgatga tgttccggaa
    25261 gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca agcgatggcg
    25321 tggagccgcc ggatgctcgg caccacccag cgtattctgg tagaaggtac atcgcgtaag
    25381 agcatcatgg agctttccgg gcgtacggaa aataaccgcg tggtcaactt cgaaggcacg
    25441 ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc gaactctctg
    25501 cgcggtaaag tggtgcgtac tgaagatgaa atgggtctgc gcgtggcaga aacaccagaa
    25561 tcggtgattg cccgtactcg caaagaaaac gaccttggcg tgggttatta tcagccgtaa
    25621 ttctcaggcc cgccgttccg gtgggccttg ttttttctct ctttatcccc atctttatcg
    25681 caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg tgaatgttca
    25741 gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc gaaatcaccc
    25801 tggagccagc agacaacgcg cgtctgttga gcctgtgcgg cccgtttgat gacaacatca
    25861 agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac tttaaactga
    25921 ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg tatgtcgata
    25981 ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac cttgcgatta
    26041 aagaagcacg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc aaagcggtca
    26101 atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg
    26161 ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg ggtaaaacct
    26221 acctggcagt ggctgcggca gttgatgccc tggagcgtca ggaaattcgc cgtattctgc
    26281 tgactcgtcc ggcagtagaa gccggtgaga aactgggctt cctgcctggc gatttaagcc
    26341 agaaagtaga cccgtatctg cgcccgctgt acgacgcgct gtttgaaatg ctgggttttg
    26401 agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcaccgctg gcctatatgc
    26461 gtggtcgtac gctgaacgac gcatttatca ttctcgatga gagccagaac actaccatcg
    26521 aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt atcaccggcg
    26581 acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat gccatcgaag
    26641 tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac gtggttcgtc
    26701 acccggtggt ggcgcgtatc gttaacgcct atgaagcctg ggaagaagcc gaacaaaaac
    26761 gtaaagcggc gctggcggca gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt
    26821 gatcctcgat ttacaactgg catgtgaaga taactccgga ttgccggaag agagccagtt
    26881 tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag tgacgattcg
    26941 cgtggtcgat accgccgaaa gccacagtct caatctgacc tatcgcggta aggataagcc
    27001 gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggagatgt cgctactggg
    27061 cgatctggtt atctgccgtc agatggttga gaaggaagct caggagcaag gcaaaccact
    27121 ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag gttacgatca
    27181 catcgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta tgcttgctct
    27241 gggctatgag gatccgtaca ttgccgagaa agaataattt gccagccatt tgactggcag
    27301 acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga gaacccatac
    27361 gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa gaagggattt
    27421 ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga cgaactgctg
    27481 gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg cgatatgctc
    27541 gaaggggtga tggacatcgc agaccaacgc gtccgcgaca tcatgatccc ccgctcccag
    27601 atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc
    27661 gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg gattctgatg
    27721 gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg
    27781 ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct gaaagagttt
    27841 cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt ttccggtctg
    27901 gtaaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga atatgacgaa
    27961 gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg cgcactggct
    28021 tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga agtcgacact
    28081 atcggtggtc tggtgatgca ggcatttggg catcttccgg cacgtggcga aaccatcgac
    28141 atcgacggtt accagttcaa agtggcgatg gccgacagtc ggcgtattat tcaggttcat
    28201 gtcaaaatcc cggatgactc accccagccg aagctggatg aataaaaccg aaactggata
    28261 gataactaca tggcttttgc ctcattaatt gaacgccagc gcattcgcct gctgctggcg
    28321 ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg gcctgcggcg
    28381 attatttcgc tgatggggct tcaggcgttg acctttaacc gccgtccact ccagtctgcc
    28441 gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg ggtctatgtc
    28501 agcatcgcga cctttggcgg aatgcctggc ccggttaaca tcttcctggt ggtactgctg
    28561 gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg cctgtggccg
    28621 aaaaccacct ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt gaccgagttt
    28681 ctgcgcggtt gggtactgac aggcttcccg tggttacagt tcggctatag ccagattgat
    28741 ggcccgttaa aagggctggc accgctaatg ggcgtggaag ccattaactt cctgcttatg
    28801 atggttagcg gcctgctggc actggcgtta gtcaaacgca actggcgtcc gctggtggtg
    28861 gccgtcgtgc tgtttgccct tcccttcccg ctgcgttaca tccggtggtt taccccacaa
    28921 ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc gctgaaatgg
    28981 gacgaaggcc agcttcttaa tacgctgaag atttactaca acgcaacggc accgctgatg
    29041 ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga aattaatcag
    29101 caaccgttcc tcaaagcact ggacggtgag ttgcgtgata aaggtagctc gctggtgacc
    29161 gggattgttg acgcgcgtct taataagcag aaccgttacg atacctacaa caccatcatc
    29221 acactgggta aaggtgcacc gtacagctac gaatcagccg atcgctataa caaaaaccat
    29281 ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt agcaccgttc
    29341 tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc gctgtcggca
    29401 aatggtattg agcttactgc ggctatttgc tacgagatca ttctcggcga gcaagtgcgc
    29461 gataacttcc gcccggatac cgactatctg ctgactatct ccaacgatgc gtggtttggt
    29521 aagtctattg gtccatggca gcacttccag atggcgcgaa tgcgtgcgct ggagctggcg
    29581 cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc gcagggtgag
    29641 attcaggcga tgatcccgca gttcacccgc gaggtgttaa ccactaacgt gacgccgacc
    29701 accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct gacggcactg
    29761 tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc ccttcttatc
    29821 gtgccggaat acatcccata tttcggcacg ctcaccggat gcggcatcag tgcattatcc
    29881 ggataacaat attctcctat ccaactttgg cacatctatt gctttgttat acaaggcaaa
    29941 gctttaacca gcatcagtac aacccggttg caccacagca atccaccggt agcaccaaaa
    30001 gagtgcattt taagaatttt gcttcggaat ggtgcggcgc acctcccaaa tataaacaat
    30061 aaaacagcaa tatctttaca tttagccaaa ctaaatgtta cctaacaggc acaacactac
    30121 acaataaagt tgcagatgat aacaacacaa acactcacaa cgggtatcca tgcgttctta
    30181 acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc aatcctggcc
    30241 ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc gggcagtacg
    30301 ctggacaaaa tcgccaaaaa cggcgtgatt gtcgtcggtc accgtgaatc ttcagtacct
    30361 ttctcttatt acgacaatca gcaaaaagtg gtgggttatt cgcaggatta ctccaacgcc
    30421 attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtgaa actgattccg
    30481 attacctcac aaaaccgtat tccgctgctg caaaacggca cttttgattt tgaatgtggt
    30541 tctaccacca acaacgtcga acgccaaaaa caggcggctt tctctgacac tattttcgtg
    30601 gtcggtacgc gcctgttgac caaaaagggc ggcgatatca aagattttgc cgacctgaaa
    30661 ggcaaagcca tagtcgtcac ttccggcact acctctgaag ttttgctcaa caaactgaat
    30721 gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag atcacggtga ctctttccgt
    30781 accctggaaa gcggtcgtgc cgttgccttt atgatggatg atgctctgct ggccggtgaa
    30841 cgtgcgaaag cgaagaaacc agacaactgg gatatcgtcg gcaagccgca gtctcaggaa
    30901 gcctatggtt gcatgttgcg taaagacgat ccgcagttca aaaagctgat ggatgacact
    30961 atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt ttgataaatg gttcaaaaat
    31021 cccattccgc cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat gaaagcactg
    31081 ttcaaagaac cgaatgacaa agcactgaac taattacaag aatcaggggc ggaaattcta
    31141 gccctctcga ttgttacgta gcacggacag actatacgcc tgatggtcgt tccccatcgg
    31201 gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa cgctgctacc
    31261 ctcttttttc tggagtagat ttatgtctat agactggaac tggggtattt ttttacaaca
    31321 agccccgttc ggcaacacca cctatcttgg ttggatctgg agtggttttc aggtgacgat
    31381 cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc ggttcatttt tcggtatttt
    31441 acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg aactgttccg
    31501 caacgtgccg ttgattgtgc aattctttac ctggtatctg gtgatcccgg agctgctgcc
    31561 ggagaaaatc ggcatgtggt ttaaggccga gctggatccc aatattcagt ttttcctttc
    31621 atccatgctc tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg tacgcgccgc
    31681 gattcagtcg ctgccgcgtg ggcagaagaa tgccgcgctg gctatgggtc tgacgctgcc
    31741 gcaggcttac cgctacgtac tgctgcctaa tgcttatcgc gttatcgtcc cgccgatgac
    31801 ctcagagatg atgaacctgg tgaaaaactc cgccatcgcc tcgaccatcg gtctggtaga
    31861 tatggcagcg caggcgagta aattgctcga ttactcggct cacgcctggg agtcgtttac
    31921 cgccattacg ctagcgtatg tgtttattaa cgcttttatc atgctggtga tgacgctggt
    31981 tgaacgtaaa gttcgcctgc ctggcaacat ggggggcaaa taatgtacga gtttgactgg
    32041 agttccattg tcccttccct gccatatctg ctcgacgggc tggtgatcac gctgaaaatc
    32101 accgtcacgg cagtcgtgat tggtattttg tggggcacca tgctggcggt gatgcgcttg
    32161 tccagctttg cgcccgtagc gtggtttgcc aaagcctacg ttaacgtatt ccgctcgatt
    32221 cctttagtta tggttttgct gtggttttac ctgatcgtgc cgggttttct gcaaaacgtg
    32281 ctgggattat caccaaaaaa tgatatccgt ttaatttcgg cgatggtggc gttttcgatg
    32341 tttgaagcgg cctactattc agagattatc cgtgccggta ttcaaagtat ttctcgtggt
    32401 cagtcgagcg ccgcgctggc gttgggaatg actcactggc agtcgatgaa actgattatt
    32461 ctgccgcagg cgttccgcgc gatggtgccg ctgctgctca ctcagggcat cgtactgttc
    32521 caggatacct cgctggtgta tgtgttatct ctggccgatt tcttccgtac cgcctcaacc
    32581 attggtgagc gtgatggcac gcaggttgag atgatcctgt ttgccggatt tgtttatttc
    32641 gttattagcc ttagcgcgtc gttgttggtc agctacttga aaagaaggac agcataatga
    32701 ttaccctgaa aaatgtttca aaatggtatg gtcactttca ggtgctgacc gactgctcaa
    32761 ccgaagtgaa aaaaggcgaa gtggtggtgg tttgcggccc gtctggttcc ggcaaatcaa
    32821 cgctgattaa aaccgtcaac ggcctcgaac cagtgcagca aggtgaaatc accgtcgatg
    32881 gtatcgtggt taacgacaag aaaaccgatc tggcaaagct gcgttctcgc gtcgggatgg
    32941 tattccaaca tttcgagctg ttccctcatc tgtcgattat cgaaaacctg accctggcgc
    33001 aggtgaaagt gcttaaacgc gataaagcac cggctcgtga aaaagcattg aaattgctgg
    33061 agcgcgtggg actttccgca catgccaata aatttccggc gcagctttcc ggcggccagc
    33121 agcagcgtgt ggcaattgct cgcgcattgt gtatggatcc tattgcgatg ctgttcgacg
    33181 aacccacatc ggcactagac ccggagatga tcaacgaagt actggacgtg atggtggaac
    33241 tggcgaacga ggggatgacc atgatggtgg tgacccacga aatgggcttt gcccgtaaag
    33301 tggcgaatcg ggtgatcttt atggatgagg gtaaaattgt tgaagactcg ccgaaagatg
    33361 cgttcttcga tgatccgaaa tcggaccgcg caaaagactt cctcgcgaaa atcctgcatt
    33421 aatcactctg gcgcgcatac ccaagtgcgc gccatctcac gctttatcgt ctcgcccctc
    33481 gtatctgtta catcctggcg ttacccttgt cgcaaagaag cacacaacaa ggagcaacaa
    33541 tggcactgcc aattctgtta gattgcgacc caggtcatga tgacgctatc gcaatagttc
    33601 tcgccctcgc ctcaccagag cttgatgtca aagcaattac gtcttccgcc ggaaaccaga
    33661 caccagaaaa aaccttacgc aatgttctgc gtatgctgac cttgcttaat cgcaccgata
    33721 ttccggtggc aggcggcgcg gtaaaaccgt taatgcgtga gttgattatc gcggacaatg
    33781 tgcacggcga aagcggtctc gacggcccgg cattaccgga accgacgttc gcaccgcaaa
    33841 actgtacggc ggtagagctg atggcgaaaa cgctgcgtga aagtgcggaa cctgtcacga
    33901 ttgtgtctac cggaccgcaa actaacgttg ccttgctgct caatagccat ccggaactgc
    33961 atagcaaaat tgcccgtatc gtgattatgg gtggcgctat gggactgggt aactggacgc
    34021 ctgcggctga atttaatatt tacgttgacc cggaagcggc agaaattgtc ttccagtcag
    34081 ggatcccggt ggtgatggcc ggtctggatg ttactcacaa agcacaaatc cacgttgaag
    34141 acactgagcg tttccgcgcg attggcaacc ctgtttcaac cattgttgcc gaactgctgg
    34201 atttcttcct cgaatatcat aaagacgaaa aatggggctt tgtcggcgca ccgctacatg
    34261 acccatgcac catcgcctgg ctgttaaaac cggagttgtt taccactgtt gagcgctggg
    34321 ttggcgtgga aacacagggg aaatataccc agggtatgac ggttgttgat tattattatc
    34381 tgactggcaa taaaccgaat gccactgtaa tggtcgatgt tgatcgtcag ggctttgttg
    34441 atttactggc tgatcgtctg aaattttacg cttaacacac gtcattgatt aagatcacca
    34501 cgcgttacct gggtggtctt aatctgttta tctctttttc tttcaagata taaatgcaat
    34561 ggataatgca gaactcgcca ttggtatcga tctcggtact accaatagtt taattgccgt
    34621 ctggaaagac ggtgccgcgc aattaattcc aaataagttc ggtgaatatt taacaccatc
    34681 cataattagc atggatgaaa ataatcatat tttagtcgga aaaccggctg tatcacggcg
    34741 tacttcgcat ccggataaaa cggcagcgtt atttaaacgt gcaatgggca gtaataccaa
    34801 ctggcggtta ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc
    34861 attaaaagaa gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctctgt
    34921 tccggcttat ttcagcgatg aacaacgcaa gcatacccgt ttagcagcgg agttagccgg
    34981 tttaaatgcg gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca
    35041 tacccaacaa aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgtcac
    35101 ggtgcttgag tacgccacgc cggtgattga agttcacgcc tccgctggcg acaacttcct
    35161 cggtggcgaa gattttaccc atatgctggt cgatgaggtt ttaaaacgcg cggatgtcgc
    35221 caggaccacg cttaacgaga gtgaactggc agccttgtat gcctgtgtgg aagcggcaaa
    35281 atgtagcaat caatcgccaa tacacattcg ctggcagtac caggatgaaa cgcgggaatg
    35341 cgaattttac gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt
    35401 gccgattgaa caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt
    35461 gctggttggc ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg
    35521 caaattaccg tatcaaagtt acgatccgag caccattgtc gcgctgggcg cagcaatcca
    35581 ggcagcctgc cgcttacgca gtgaagatat tgaagaagtg atcctcactg atatttgccc
    35641 ttactcgttg ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct cgccgattat
    35701 tgaacgaaac accactgtgc ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga
    35761 acaggattct attacggtta acgtctatca gggagaaaac cacaaagtta aaaacaacat
    35821 tctggtggaa tccttcgatg tgccgttgaa gagaaccggg gcttatcagt cgattgatat
    35881 tcgctttagt tatgatatca acggtttgct tgaagttgac gtgcttctgg aagacggcag
    35941 cgttaagtcc agagtgatta accacagccc ggtaacattg agcgcgcagc agattgaaga
    36001 gagtcggacg cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaaccgcac
    36061 ctttaaggcc aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat
    36121 tggccgggtg atcaccgatt ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga
    36181 tgaggtcagg cggcaggcga gcgtttattt agccattgag atcccataat cacggctgcc
    36241 ggatgcggcg taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa
    36301 catcgcgatt ttgccgagag cataacctat caccagcacc gttaagctga ttttgataat
    36361 gctggtaatg ctctgtggtt tttcatcctc atcatttttt tcttcatggt tttcaggtgt
    36421 tggctcatcg acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt
    36481 gataatggcc tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag
    36541 catctcttgt ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag
    36601 taacggtgaa tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt
    36661 gcgtttgaag aatagcgact caataaacga ttgacctaac ggcgatagtt ttccgctatg
    36721 taaaatttcc gcccataaca gacgcgtccg ggcgctctca ccaggaacat atttcaacgg
    36781 tttaccttgc gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata
    36841 atccgggcaa tgttgtgcgc tccactgcaa caacaaatta tcggcttgtg tagatgggta
    36901 acttccccag tacgcgcaga gatcggtaag caggctttcc ccctcgccgc agtacaaacg
    36961 ttgtgcacac tggtaatacc gggcatcttc gctttctggt tcttccattt gccactgttc
    37021 ggctacagga atgagttccg ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt
    37081 gcgatgtaac tttgcatcat caggaatgaa ccacggacct tccatcgcca aaaacgcagt
    37141 gacatagtca ggatggtaac ggcagcaacg ttccagcgcg aagtaatact caacagtctg
    37201 gttctgcaac gcaacgggat ataccagaag gccaaggaaa tcgaaacaat caccagcctg
    37261 aatggcctca agaaaagcct ccctttctta ctcatctatt ccttcgctgt cattaaacga
    37321 atgccagttc aaacgctctg ccagcaaaga agcacaattt aatgaaagat atcgcgcttc
    37381 tatagcgatt gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt
    37441 taattgctga ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag
    37501 taatgccctg aatacacgta gtatttcatg ctcagtggca tcatcttttt cttcatcatc
    37561 tgcttcctcg acaggattta ccgccagtcg cagggcctct tcataggcct ggcgtaatgc
    37621 tttaaaccct tgcggatcgg tttcgggatg acataacggt aagcgagcaa gataagcctg
    37681 gcggataatg tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat
    37741 aggtcgctca catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag
    37801 ggaagcgtgg catagaaacg atcgcttccc gcagccagct cttcgaacat ccgactcccg
    37861 gcgctatcgt atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcattga
    37921 gaagattgcc agtagagacg ccctagtgaa taggcggaaa aatagtgcat ccagctgtga
    37981 tagtaatggt gagctcgttc atagacgcgc gattgcagcc ataaactctc gtcttccgtc
    38041 agccacttgt tgagcacacc gacccgtgac agaaacccca ttcgaacata atcccaagct
    38101 ttgattcccc cttccccaca acacatagcg gtttgggcaa cgaattgggt ataaatcaac
    38161 cctctctccg agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat
    38221 gtcagatata agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc
    38281 tgaattaaat catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc
    38341 ccggtataca tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg
    38401 ggcgcagaaa gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt
    38461 aagtgcatta actg
//
LOCUS       DBFIFS010000023        37887 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.023, whole genome shotgun sequence.
ACCESSION   DBFIFS010000023 DBFIFS010000000
VERSION     DBFIFS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.023"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            123..2366
                     /gene="pdeF"
                     /locus_tag="N7A07_001067"
     CDS             123..2366
                     /gene="pdeF"
                     /locus_tag="N7A07_001067"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU2475772.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2405..3946)
                     /gene="ppx"
                     /locus_tag="N7A07_001068"
     CDS             complement(2405..3946)
                     /gene="ppx"
                     /locus_tag="N7A07_001068"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU2475773.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3951..6017)
                     /gene="ppk1"
                     /locus_tag="N7A07_001069"
     CDS             complement(3951..6017)
                     /gene="ppk1"
                     /locus_tag="N7A07_001069"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU2475774.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6188..6826)
                     /gene="purN"
                     /locus_tag="N7A07_001070"
     CDS             complement(6188..6826)
                     /gene="purN"
                     /locus_tag="N7A07_001070"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU2475775.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6826..7863)
                     /gene="purM"
                     /locus_tag="N7A07_001071"
     CDS             complement(6826..7863)
                     /gene="purM"
                     /locus_tag="N7A07_001071"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU2475776.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8188..8814
                     /gene="upp"
                     /locus_tag="N7A07_001072"
     CDS             8188..8814
                     /gene="upp"
                     /locus_tag="N7A07_001072"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU2475777.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8900..10189
                     /gene="uraA"
                     /locus_tag="N7A07_001073"
     CDS             8900..10189
                     /gene="uraA"
                     /locus_tag="N7A07_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU2475778.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10284..10985
                     /gene="hda"
                     /locus_tag="N7A07_001074"
     CDS             10284..10985
                     /gene="hda"
                     /locus_tag="N7A07_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU2475779.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11123..11482)
                     /gene="arsC"
                     /locus_tag="N7A07_001075"
     CDS             complement(11123..11482)
                     /gene="arsC"
                     /locus_tag="N7A07_001075"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU2475780.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11503..12966)
                     /gene="bepA"
                     /locus_tag="N7A07_001076"
     CDS             complement(11503..12966)
                     /gene="bepA"
                     /locus_tag="N7A07_001076"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU2475781.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13179..14240
                     /gene="yfgO"
                     /locus_tag="N7A07_001077"
     CDS             13179..14240
                     /gene="yfgO"
                     /locus_tag="N7A07_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU2475782.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14278..15126)
                     /gene="focB"
                     /locus_tag="N7A07_001078"
     CDS             complement(14278..15126)
                     /gene="focB"
                     /locus_tag="N7A07_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU2475783.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15148..17160)
                     /gene="hyfR"
                     /locus_tag="N7A07_001079"
     CDS             complement(15148..17160)
                     /gene="hyfR"
                     /locus_tag="N7A07_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU2475784.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17190..17603)
                     /gene="hyfJ"
                     /locus_tag="N7A07_001080"
     CDS             complement(17190..17603)
                     /gene="hyfJ"
                     /locus_tag="N7A07_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU2475785.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17596..18354)
                     /gene="hyfI"
                     /locus_tag="N7A07_001081"
     CDS             complement(17596..18354)
                     /gene="hyfI"
                     /locus_tag="N7A07_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU2475786.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18351..18896)
                     /gene="hyfH"
                     /locus_tag="N7A07_001082"
     CDS             complement(18351..18896)
                     /gene="hyfH"
                     /locus_tag="N7A07_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU2475787.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18906..20621)
                     /gene="hyfG"
                     /locus_tag="N7A07_001083"
     CDS             complement(18906..20621)
                     /gene="hyfG"
                     /locus_tag="N7A07_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU2475788.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20611..22191)
                     /gene="hyfF"
                     /locus_tag="N7A07_001084"
     CDS             complement(20611..22191)
                     /gene="hyfF"
                     /locus_tag="N7A07_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU2475789.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22196..22846)
                     /gene="hyfE"
                     /locus_tag="N7A07_001085"
     CDS             complement(22196..22846)
                     /gene="hyfE"
                     /locus_tag="N7A07_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU2475790.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22858..24297)
                     /gene="hyfD"
                     /locus_tag="N7A07_001086"
     CDS             complement(22858..24297)
                     /gene="hyfD"
                     /locus_tag="N7A07_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGU2475791.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24314..25261)
                     /gene="hyfC"
                     /locus_tag="N7A07_001087"
     CDS             complement(24314..25261)
                     /gene="hyfC"
                     /locus_tag="N7A07_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU2475792.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25272..27290)
                     /gene="hyfB"
                     /locus_tag="N7A07_001088"
     CDS             complement(25272..27290)
                     /gene="hyfB"
                     /locus_tag="N7A07_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU2475793.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27290..27907)
                     /gene="hyfA"
                     /locus_tag="N7A07_001089"
     CDS             complement(27290..27907)
                     /gene="hyfA"
                     /locus_tag="N7A07_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU2475794.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28160..28630)
                     /gene="bcp"
                     /locus_tag="N7A07_001090"
     CDS             complement(28160..28630)
                     /gene="bcp"
                     /locus_tag="N7A07_001090"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU2475795.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28630..29202)
                     /gene="gcvR"
                     /locus_tag="N7A07_001091"
     CDS             complement(28630..29202)
                     /gene="gcvR"
                     /locus_tag="N7A07_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU2475796.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29348..30226
                     /gene="dapA"
                     /locus_tag="N7A07_001092"
     CDS             29348..30226
                     /gene="dapA"
                     /locus_tag="N7A07_001092"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU2475797.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30243..31277
                     /gene="bamC"
                     /locus_tag="N7A07_001093"
     CDS             30243..31277
                     /gene="bamC"
                     /locus_tag="N7A07_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU2475798.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31490..32203
                     /gene="purC"
                     /locus_tag="N7A07_001094"
     CDS             31490..32203
                     /gene="purC"
                     /locus_tag="N7A07_001094"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU2475799.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32371..33234
                     /gene="ypfJ"
                     /locus_tag="N7A07_001095"
     CDS             32371..33234
                     /gene="ypfJ"
                     /locus_tag="N7A07_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU2475800.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33249..35264
                     /gene="tmcA"
                     /locus_tag="N7A07_001096"
     CDS             33249..35264
                     /gene="tmcA"
                     /locus_tag="N7A07_001096"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU2475801.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35338..36036
                     /gene="ypfH"
                     /locus_tag="N7A07_001097"
     CDS             35338..36036
                     /gene="ypfH"
                     /locus_tag="N7A07_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2475802.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36117..36317)
                     /gene="ypfN"
                     /locus_tag="N7A07_001098"
     CDS             complement(36117..36317)
                     /gene="ypfN"
                     /locus_tag="N7A07_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU2475803.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36345..37472)
                     /gene="dapE"
                     /locus_tag="N7A07_001099"
     CDS             complement(36345..37472)
                     /gene="dapE"
                     /locus_tag="N7A07_001099"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU2475804.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37476..37832)
                     /gene="yffB"
                     /locus_tag="N7A07_001100"
     CDS             complement(37476..37832)
                     /gene="yffB"
                     /locus_tag="N7A07_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU2475805.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         9207 a         9828 c         9981 g         8871 t
ORIGIN      
        1 actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag
       61 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
      121 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
      181 tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
      241 ccggtgaggt ttttctcttt tatctgcctc tggcactgat gatcagcatg atgatgtttt
      301 tcagctgggc ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc
      361 tgggttttta cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt
      421 ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt
      481 taatttccca gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt
      541 tccagtttgc tgcatttgta ggattactgg cgagcagaga aaatctggtt ggcgtcatgc
      601 cttttaatct cgggacctta atcaattatc aggccttact ggtgggcaat ctgattggtg
      661 tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc gttttattta cgtagttatt
      721 attcgcaatt aaaacagcag gttgatgtca aagtcaccaa aaaagagttc gcactctggc
      781 tgctggcatt aggtgcttta ctgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa
      841 tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc
      901 gctatggtta taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca
      961 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
     1021 ccagttatct ggtattctct tttattgtca attatatggc tgtactggca acccgtcaga
     1081 gaacggtagt cagacgcatt cagcgccttg cgtatgtgga cccggtggtt catctgccaa
     1141 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
     1201 gcatccctgg catggaaatg ctggttaaaa actatggcat catgctgcgg attcaataca
     1261 agcaaaaact ttctcactgg ctgtcaccct tgctggaacc aggtgaagat gtttatcagc
     1321 tttcgggtaa cgatctcgcg ctgcgactga atacagaatc gcaccaggag cgcattaccg
     1381 cactggatag ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc
     1441 agattggcgt cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg
     1501 gagagctaaa tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc
     1561 gtcgcggggc aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac
     1621 tacagcaggc gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc
     1681 gtggtgatgt ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactaatcg
     1741 gccccgatag cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt
     1801 gggtcattga gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc
     1861 gttttgctat taatctgtct ccaacctcgg tatgtcaggc tcgttttcct gttgaagtca
     1921 gtcagttgct ggataaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa
     1981 gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag
     2041 gctgccagat tgcgattgat gatttcggca ccggctacgc cagctatgcg cggcttaaaa
     2101 atgtgaatgc cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata
     2161 gtctggatta tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg
     2221 tagtggcaga gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg
     2281 attatatgca gggttatctt attggtaagc cgcaaccgtt aattgatacg ctgaatgaaa
     2341 tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa
     2401 agcattaagc tgcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca
     2461 cgccttccca gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa
     2521 accagtcatg cgggaaacgc agtgtccagt gactgtcatc agtaatcagc gtcaatgttg
     2581 gcggtgtggt ggttgcctga cgctgattgt tgagtaatac gccaaggcgc aatagctgaa
     2641 tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagtttaa
     2701 tcgctttacg gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac
     2761 ccggcaagtc gctgttttgt agaatataag cggagtgacg atgcaaaccg ctgtggttga
     2821 tattcaaccc gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat
     2881 gcgccagctt cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca
     2941 cccgtcgggc ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac
     3001 tacgcacatc ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg
     3061 ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg
     3121 ctagtccagg aacgaagact gttttccgct cttcggaaag acctggtaaa ctcagcgatg
     3181 cgaaattacg gtgccgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta
     3241 tcccgtcttt ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag
     3301 cgcccattgc cacgttccag ccctgaatac gaaattgcca ggttaaagtt tccagttttt
     3361 gtgctgccgc catgcgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaaat
     3421 aaagctgggc aaagctgaca caccccatcc ggcggctttc aacgagaatc ggttcgaaat
     3481 tttcaccaat caccagttct gtagaaccgc cgccaatatc aataaccagt ttgcgacctt
     3541 tttccggttg ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa
     3601 taatttcaat cgggtaggga atgacctttt ccgcacgttt cagaaagtca gtggcgttca
     3661 gcgcctgacg cagcgtatgg gtaccaacta tacagacgct ggcaggagaa aacccttgta
     3721 gccgttcggc aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca
     3781 tattatctgg ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct
     3841 gcatggcacc atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga
     3901 ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt
     3961 tgttcgagtg atttgatgta gtcataaatc gccaactgcg cccgtacttt gcggcgattg
     4021 ccgcggggaa cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg
     4081 ctgaacaata tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggc
     4141 gtcgccactt caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag
     4201 acctttttat cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca
     4261 acaatactga tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac
     4321 attccgcgaa ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca
     4381 accaggcctt tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc
     4441 tgcgcgttgg cgatctcgcg gtccaccatt tcatacaaca ggcggcggga gttttgcggc
     4501 gacaccatta aataatcaaa tgtcaccgga cggtatgggt tttcaataaa gttaaatacc
     4561 cgccgtactt cgttggtgat gcgcgcatcg gcggtcagca acgaatagtc agtataaaga
     4621 cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg cataacgcac cacttcaccg
     4681 ttttctttac gtgaaatcag aaacagtttg gcgtgaattt tcagccccgg cgcagagaag
     4741 ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtca
     4801 aaacgtgcct gtagctcaac caccacggtg actttcttac cgttgtgtgc ggcgtggatc
     4861 atcgagtcga tgatgcgtga atcttttgcc acacggtaaa tgttgatttt aatcgccagc
     4921 acgctcggat cgaacgaggc ctgacgcagc agttccagca catgctcaaa ggtgtgataa
     4981 ggataataga gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct
     5041 ttatcaaacc aaatatggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg
     5101 acattgggga aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag
     5161 tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg
     5221 cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc
     5281 aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc
     5341 ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcaagg
     5401 cagtaacgca gaatgttatc cagaagaatc attggcttgc gtcgacgcgg cgcttctggc
     5461 ggtaaattca caaagcgcgg cactttatct gatgggatct ccagaagcgc gtaacggatg
     5521 gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact
     5581 aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa
     5641 taatgacgca gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc
     5701 tggttgcgcg ccatctccag caatagctcg ttgtagaggc cgtcgaattc ctgatcggct
     5761 ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt
     5821 tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca
     5881 aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc
     5941 gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat
     6001 agcttttcct gacccattac cacttttact ccgttttatt cacgggacac tgcctggata
     6061 ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct
     6121 ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta
     6181 cggggcatta ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg
     6241 cggcgttttc gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa
     6301 tggcgtgttc ctgggtttgc acgcgggcgg tgacgtcatc ctccgtatca ccagcaaata
     6361 ccgggacttt cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca
     6421 ccgatgtacc gtgctcttca tcgccatttt ccagcgcctg acgatgggga tgtaatccgg
     6481 gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa
     6541 atgccgggct gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt
     6601 cgatttcatg aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga
     6661 gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat
     6721 tgctgaaaac tgcccgtacg gtgcctttaa ttttgttggt tttacaggcg tcaataattg
     6781 cctgcaaatt acttccgttg ccggaaataa gcaccacaat attcattatt cgataaccac
     6841 gcgttgttcg gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc
     6901 attgagcagg gcgagggctt tgtccacttc cggagcaggc agggcaataa tcatcccgac
     6961 gccgcagttg aaggtgcgat acatttcatg gcgctcaacg ttaccagccg tttgcagcca
     7021 gttgaacact tccggccact gccaggagga ttcatcaatc actgcctggg tattatctgg
     7081 caatacgcgc ggaatgtttt cccaaaagcc gccgccggtc aggtgcgcaa tggcattaac
     7141 atcgaccttt tcaatcaact ccagcactga cttcacgtaa atgcgggtcg gtgccagcag
     7201 atgatcggct aatggcttac catcaagttc ggtggtttgc ggatcacaac cgctgacttc
     7261 aagaattttg cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac cgagtgcaat
     7321 cagcacatcg ccgtcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc
     7381 cacgcagaaa cccgcgacat cgtaatcctc accgtgatac atccccggca tttctgccgt
     7441 ttcgccaccc accagtgaac agcccgattg cagacaacct tccgcaatgc cgctgatcac
     7501 cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg
     7561 ttctgcacct tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt
     7621 gtcgtgacgt tttaagtcca ttgccagacg cagcttggta cctacgccgt cagtgccgga
     7681 aaccagcacg ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag
     7741 accgcccatc acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag
     7801 agcattaccc gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt
     7861 cactgcttgg gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa
     7921 cagggaaagc aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttcccca
     7981 ggcgcgcggc gaaagaagac ttgtgccagg gcaaaggtta gttttcggat ggaataatct
     8041 tctttcataa ccatttgaat ataaaataac tttatctcaa accgttatca ttttgactaa
     8101 agtcaacgaa aataatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt
     8161 gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc
     8221 aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc
     8281 gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa
     8341 gtaactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt
     8401 accgttgtgc caattctgcg tgcgggtctg gggatgatgg acggtgtgct ggaaaacgtt
     8461 ccgagcgcgc gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta
     8521 ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca
     8581 atgctggcaa ccggtggttc cgttattgcg accatcgacc tgctgaaaaa agcgggctgc
     8641 agcagcatca aagttctggt gctggtagct gcgccagaag gtatcgctgc gctggaaaaa
     8701 gcgcacccgg acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga
     8761 tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata
     8821 aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca
     8881 taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact
     8941 tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct
     9001 ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac
     9061 gctgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc
     9121 ctttatttca ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt
     9181 tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg
     9241 ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct
     9301 ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc
     9361 agactccaaa accatcatca tctctattac caccctggcg gtcacggttt taggttccgt
     9421 gctgttccgt ggtttcctgg caattatccc gattttaatt ggcgtgctgg tggggtacgc
     9481 gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc
     9541 gctgccaacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc
     9601 ggcgttagtg gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa
     9661 aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcttgtcgac
     9721 cgtgatctcc ggcttctttg gctccacgcc aaacaccacc tacggggaaa acattggcgt
     9781 gatggcgatc acccgtgttt acagtacctg ggttatcggc ggggcggcga ttttcgctat
     9841 cctgctttcc tgcgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat
     9901 gggcggcgtt tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat
     9961 cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat
    10021 catcggcgtc agtggcgcga aggtaaacat tggcgcggcg gagctgaaag gtatggcgct
    10081 ggcgaccatc gtcggtatcg gcttaagttt gattttcaaa ctgattagtg tgctgcgccc
    10141 tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg
    10201 ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt
    10261 gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc
    10321 ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggccg
    10381 cgctgcaaaa cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag
    10441 gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg
    10501 cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta
    10561 tggagcattt gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt
    10621 gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa acacggttgt
    10681 tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc
    10741 gactcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg
    10801 cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct
    10861 tgctgaagcg gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc
    10921 gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt
    10981 tgtagataag gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt
    11041 taatccatag aattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct
    11101 tcaactagcg atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac
    11161 caatccgcgc tttgccattc gcgaccacaa tcgggcgctc catcagcttc ggattatcca
    11221 ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat
    11281 aaagatcctc tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca
    11341 aatcgcgcag cgttgccgca tcggcgggtg tctcaaggta gagcaccact tctggctcca
    11401 cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt
    11461 agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg
    11521 cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg
    11581 caggctgcct aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag
    11641 tcgtccggcg agcgcataac cttctgctcg cgcagccagc tcctgatcgc ggttatttag
    11701 cgcggcttcc gcctgtgcca gcaaatccca gccgttgctg tcatctttat tattaaaggt
    11761 gtagcgatta agaatattgg ccgcttcttg tggttgaccg ccttgtagat aagcgttcgc
    11821 caggttgagc tgcaacacag gattggtgcg caaatcgcgg gcatttttca gacgattgat
    11881 cgcctcattg gctttgtttt gcccaagatc gatatcagta gccagatcga gataccatgc
    11941 gttgccaggt tctgccgcca gtaacggttg cagcgttttt cgcgcctcgt cgtatttatt
    12001 ggcttccatc gcctgtaaag cacgaccata ttgcgccgct cgttgctgac gaacgtttcc
    12061 tttcgcccat tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc
    12121 cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcgggcgcat
    12181 ctgattagca cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcagtaa
    12241 aatttccggc gggcgcgagg agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg
    12301 catcgcctgc ggatcgaatc ccgagcgttg cagcacctga ataccaatgc ggtccgcttc
    12361 ctgttcattt tgctgggtga aactgatcat cccctgacgc gttcccgcca gtgtaccggt
    12421 cagcgccgcc atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc
    12481 gacccaggtc agcggcgcgt tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg
    12541 ggtgacgtgg gagatttcgt gcgccataac tgaagccagt tgactttcgt tatcggaata
    12601 acggaacagg gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc
    12661 gtcgttgttg atcagaaaga aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag
    12721 acgcatcccc agcgaattaa tatattgcgt taacagcggg tcattaatta acggcgcgct
    12781 gccgcgtagc tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt
    12841 gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa atgccggggc
    12901 tacctgacca atggtcatag cagcaatgag ggttgcaacc aggttttttt tcaactgcct
    12961 gaacataacc tctatcctgt atttctgaag atagtcattt gaccgatact gtgaaatatc
    13021 gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gcccactcaa atgacttttc
    13081 agtttcctga atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg
    13141 gatcgcaatc ccgctcttga ggttcaggga aggtctttat gctcgaaatg ttgatgcaat
    13201 ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg
    13261 gatttggcat tatctttttc tttagtggcc tgcttgctcc gttgctggtg gctattgtgc
    13321 tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct
    13381 gggcgacgtc gattgtattg gtggttttcg tcggtatatt gctactgatg gcgttcgtgg
    13441 tactgcctat cgcctggcag cagggcatct acttaatccg cgatatgccg gggatgctca
    13501 ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgtta atggatgcgg
    13561 gcattattga tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg
    13621 tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc tatctggtgc
    13681 tggtaccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg aatgccgttc
    13741 gccgggtgct gccgcgtaac cgtggactgg caggacaggt gtggaaggag atgaatcaac
    13801 aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtagtaggg atcgccacct
    13861 ggctgggatt cttactcttc gggctgaact attcgctgct gctggcggtg ctggtcggct
    13921 tctcggtact tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg
    13981 cgctattcca gtttggtgca ggcactgaat tctggagctg tttcgcggtg tatctgatca
    14041 ttcaggcgct tgacggcaac ctgttagtac cggtgttgtt ctccgaagcg gttaacctgc
    14101 atccgctggt gattatttta tcggtggtga tcttcggtgg tttgtgggga ttctggggcg
    14161 tattcttcgc cattccattg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg
    14221 ggcaaatagc gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca
    14281 ggattcctga cgtaaataaa tagcccgata acaaatactc accagcactg caccgccgat
    14341 aatattaccc agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc
    14401 aggaaaattg tctgcgctac tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg
    14461 aatggcaatg gcgaacggaa tcacaaacaa attggcgata cagtgctcaa agccactggc
    14521 gacaaacagg gtgatgggca aaatcatggc gacgattttg tcgcataacg aacgcccgca
    14581 ataactcatc cacagcgcca ggcaaaccat cagattgcac ataatgccga ggctgacaga
    14641 ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat
    14701 cgcgttttca ctcatcacca gcccggaaaa ccagattaac aaactgaaac aggcaatacc
    14761 tgccagatta ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc aactaataac
    14821 gccccgactt tttgccatca ccgtcattac cgacgaggtg aacaggctgg tgccgcaaac
    14881 cgccagcaaa ataaagccga gtgtaaagca aaggccgccc accagatggg ttaatgcctg
    14941 tgaagacggg gcatcggcga taacggaaag gtaaaaagta aatccgatcg ccataaacac
    15001 gccagcggac atcgccatta aaaagaccga cactttactg cgggcaattt tatgggcggc
    15061 aatccgttca gcgattgccg cttttctggc gctcaactgc aagtcgaaag agagtttgtt
    15121 tctcatgcat actcctgcaa aagcagatta caacacctcg cgaaccgaga tccccagacg
    15181 ctgcattcgt gacagcagcg tggtgcgctt catccctaat cgtgtcgccg cgccacgggg
    15241 cccggcaaca atgccattgg tttctcgcaa tacctgaaca atgcgctgac gttcttcttc
    15301 gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa gcgctgaatc
    15361 ttcatttagc gtcggcagta aacggctttg tcggacatta agatgtaaat tcagactgtt
    15421 accgcgagtc agcagtaccg ctcgctcaat cacgttttcc agctcgcgca cgttgcccgg
    15481 ccaatcccac gacatcaact ggcgtagtgc ctcggtcggg atggcgtcaa ttgcgcgatt
    15541 catatggcgc gccatttttt gcgtgaaatg ttttgctaaa agagggatat cttccggacg
    15601 gtctcgcagc ggcggcaatt ccagtgggaa gacattcagg cgataaaaga gatcgctgcg
    15661 aaactggcga tcttcaacca tttgccacaa atcacggttg gtggcggcaa tgactctgac
    15721 attcaccggg atcgttctac tcccgccgag acgctcaatt tcccgctcct gcaatacgcg
    15781 cagcaattta ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa acaacgtacc
    15841 gccatcggca atttcaaaac gaccacgatg ggtattaatc gcaccagtaa aggccccttt
    15901 gtcatgaccg aataactcac tttccagaag actggcgggg atggcagcgc agttgatttt
    15961 gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga tcacctcttt
    16021 gccggttccg gtttcaccac aaatcaacac cgtactgtcg ctcttcgcca caatatctac
    16081 ctgctggagc aggtcttcca ttgcctggct ttgatagata atgtcaccga cgcccagatt
    16141 cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc aaactttcct gcaaatcggt
    16201 catgctacgc caggcatcgg cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg
    16261 tagtagctgg cagttttcct cactaaagag agtggtagag gtatgcgcca gcaacaatgc
    16321 acccggtgta tggttgccaa aggtaagcgg tatcaggagc gaagattcgc agccgttaga
    16381 tgcaagtaag tgtaataacg ggtcgcgttg ccagagaaac agatcgtctg cacggtgcgt
    16441 caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc caggcatatt
    16501 gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc tgaacttctc
    16561 gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa aatgatggat
    16621 ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca ctgcattagt
    16681 gatatccacc agtacccgat gatgatcgcg atctttctcc agcaaacgca gcgccacctg
    16741 ttgttcttca tcattgagcc atgattgcag aaccggggtg ataatgcgtg ccagccagtt
    16801 aaacagtgtg caatcctggg gagtaaaacg actgccgtgg gtgcgaataa agcgtagctc
    16861 cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgacca tctcgccctg
    16921 atactctgtt gttgccgtca catcgctgcc atcatcatgg tagcggtaat agaagttgct
    16981 tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca gcgggatgaa
    17041 ggcgcgtaat aaagacgcct tgccgtgttt ctgcgctaac cgctctgcga gcattccgtt
    17101 taccgcttca attgttattc cttgcggcgg ggcaaacatc gcctcgtctg acatagccat
    17161 caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat cagatagata
    17221 gccggttcca gcacaatttc atccaacaga tgaatgaatt gaatgctacg cgcttgccac
    17281 acagacgtcg cgtcatctgc cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg
    17341 gcgtcaataa caatctcacc aaaagtcatc acccccagca tcttacgtcg ggcttgctcc
    17401 tcttcgacca gtgcaagcca atcttcgtat tccgtcagtg ggcagcggaa ggtgacattc
    17461 agacaatcaa tcacgccaac gtgatgaccg atagccagcg agtaatacat tacctgggag
    17521 ctgtgttccg gcatctcgtc gctactggcg acaaactttt ttcgcagcgt ccagaaaaca
    17581 atttccccgc actcttcagt catgtaaccc ccggagtacg cgaaagagtt gctgaacgat
    17641 actattgaga cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat ttcctgtagg
    17701 atcgttgctt aaatggcgca ggagccgatc gcaaatttct tgcccctgac gatagcccgc
    17761 cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct gcggccacaa
    17821 cggttgcata gtcaccccgg tgggatcgcg ataatccacc gcgtgaatct tctgttgcag
    17881 caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg
    17941 gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt agagatcgtg
    18001 gaaaataccg ccgccgacac cgcacgcgcc gtaggaaaca cagattttat gatcgggggc
    18061 agactcatac gcccgaagtg caggcatacg catcgcccgg gtgactgcgc cagtaaataa
    18121 caaaatatcg gcgtgacgcg gtgatgaaac aaccttaatg ccaaaacgtt ctgcgtcgaa
    18181 tacgggtgtg atggcggcaa aaatttcgat ttcacaggcg ttgcagccgc cgcaatcgac
    18241 gcgataaacg taggctgagc gacggatatc ctgtagcaaa tgccgcttca tcttttgcgt
    18301 ttgctcgtcc agcgtgatgg gctggctgac atgttgtgta agcactggac tcatagctgc
    18361 tccttagcca ccagcggtac atctgtatcg tcgttgatca gcgtcgcgcg ttgtttgcac
    18421 tccgggcaga cgctcgcttg cgcccgcaac atttcgcggt tttgtggcgc attttgttgc
    18481 tgtgccaaca attcagcagc cagtgcgacg gttttttgcg gggcaaacgg gcgttcgcaa
    18541 cggctgcaac gttgtagatg gaacgtcgcg cgggtataga gatcggcttt attggtgacg
    18601 gtcagttcaa agttattggt aagctggatg gctctggtcg ggcacacttc ttcacaacgt
    18661 ccgcagtaaa tacaacgccc cagatagagc tgccaggtgc gcgaattttg ctggtcgtcg
    18721 gtctggatag tcagcgcatt tgtcggacaa gcacaggcgc aggcaccaca ggcaatacat
    18781 tggctgggca tcaggtccgg tttcccgcga aagcctgggc tgacctccag tggcgcgaag
    18841 ggatatttca ccgtcgcggt tccggcgcgc ataatagttt tcagtaactt cagcatcttc
    18901 tgtccttact tcagcggcga acggttacga tcaatgccgt agcgttcgat ctctttatac
    18961 ggcacggttt ttgactggcg cttgcgcaca tcaaccagcg tcacacggtc ggtacaggag
    19021 tagcagggat caaggctacc gataatcagc ggtgcgtcag aaacggtatt gccgcgcagc
    19081 atgtaacgca acaccggcca gttggcgtag gtggcggcac ggcagcgcca gcggaacaat
    19141 ttttggttat caccgagcat gctccagtgc acgtcttcac cgcgtggcgc ttcaacaaag
    19201 ccgagcgcga atgcgtgagg tttatagcta aagccttcgg tcagcagtgg ggtatccggc
    19261 atgttgtcga gagcaaattc cagcattgcc agcgaatcaa acgtctcttt gacacggacc
    19321 atcacgcggg agaaaacatc gccgccggtg aaggtaaaca gtgtttttgg aatattgccg
    19381 tagtcggcgt aggggtgatc aaagcgcaaa tcacgggcaa aaccactgcc gcggatcagc
    19441 ggccctacag ggctataatc acgggcgatt tgtcggtcga gaatgccaat gccctgagtg
    19501 cgttgttcca tattcggcgt ggcaagcagc atttctacca gctccgacac gtcggcgcgc
    19561 atctcgcgca ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca
    19621 ccaccaatca gattcagacc gtaggtttta cgcgacccgg tcagcaattc cgccattgtc
    19681 atcgactttt cccgcacgcg gaaaaattgc ataaagccgg tatcaaatcc aacaaaatgg
    19741 caggagaggc caaggttgag caaatgactg tgtagccgtt cgacttccag cagaatcgag
    19801 cgaatggtat gcgctcgttg cggcacctca atccccagtg cattttcaac cgagttggta
    19861 taggccacac tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact
    19921 tcgttgtagc ccatccgcgt ttctgccagt ttctccatgc cgcgatggac ataaaacaga
    19981 cggtaatcag catcgacaat ttgctcgcca tccacaaaca agcggaagtg acccggttca
    20041 tcggaggtga tatgcagcgg gccgacaggg atcacccgcg caccgctgtt gccctcattg
    20101 ataaacggat acgtttcgga atcagtcgtc ggttcagggc gcagtcgata atccatcgca
    20161 tctttgcgca gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgctga
    20221 tccggcaggc caaccggaat cagcccgtac atatcacgaa tttctcgctc gccccagacc
    20281 gcggcaggga cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagcgcc
    20341 ttcaccacaa tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa
    20401 tgaccgttaa gtgtccgctc gtcgttgcca aacaggaccg gaagccagcc atcatgttga
    20461 taataaagat actgtacaac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt
    20521 tcaggcgttt gtcgctcttc atccagcacc gcgccgggga actgcgtttt cagggcggcg
    20581 agaatcgctt cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga
    20641 tgcggtgcct gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt
    20701 ccctgagagg actatagtgg aagcgcccgc caggatcctg atgacaggtt gtggaatatg
    20761 cgttcccatc gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc
    20821 gagatcaccc cggttaacgg cctgcggcgg tttcgccatt aacacccgcg cagccatccg
    20881 taccaggccc gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg
    20941 attacgtgcc agtccggcgg taacggtcat aaattcgcta agaaaaatgt tgaagggcgg
    21001 catccctgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt
    21061 gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga
    21121 accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa
    21181 aattcccagc ggcccgccaa tgcctagcgc caccgcgacc agtcccatgt tctccacgct
    21241 ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc
    21301 aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg
    21361 gcaaataatg atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga
    21421 gagtagggcg ctgaccggac tcggcgcttc actgtgagca tccggcagcc aggcgtgcat
    21481 gggaaatagc ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat
    21541 taatgttgga tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc
    21601 tgcctgcggc ataacgctgg cggcgttggc gtatactagc acggtaccga acagaccaaa
    21661 agcgacacca acagtacaaa taatgatgta cttccatgct gcttccagcg atgaacgctg
    21721 accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgatcg ccgcccacat
    21781 cacaatcagg ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa
    21841 accgtagtaa tcgcacagcg taacgggtga aagctcgccg tgctcaactt catgacgcat
    21901 gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa
    21961 accgcccaga ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc
    22021 ggcagtttgg accacccaga gtgccagaat cagcagcagt gtgatcccta agctatgtaa
    22081 tactgtcacc gtgcgagtcg cagagtgccc tcgtttccgg caggcaaaac agagcagcga
    22141 aaaaagcagc ggagtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc
    22201 ccttcagcgc ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg
    22261 ccagtaacac catcacaatg acggcgaaga tggcgtcggt agcgatacca atttccacca
    22321 gttccggtgc acgccaggct agaagcgcca gcaccagatg ggagccgttt tccatcaggc
    22381 agtaaccaaa aatttgccgc aggatattgc gctggctaac aatgcacagc aggccaagca
    22441 gaaaatgacc taacgccacc gccagcgctg gtttcagccc ggtagccatc gatagcttca
    22501 cgggctgaac gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg
    22561 ccggaccgaa taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca
    22621 tgattaacgg taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt
    22681 gctctgcggc gaacaggcac gagagggtgg cgaaaataga caccagcacc agcgactggc
    22741 aggcgtaaaa tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca
    22801 gcatcatcag tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta
    22861 ctgcaaccag gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcag
    22921 caccaggcgc attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc
    22981 ggggacgacg cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat
    23041 cagcagaatc atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcagc
    23101 aaacagcggg aatttactga agaagccgtt gaacggcggt acgccggtaa tcgccagcgc
    23161 tgccacgcag aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg
    23221 caacaagcgc gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt
    23281 agcgaacgcg tggttgacga tgtaggcgat gctgccctcc agcgccagcc gcgagccgaa
    23341 gatggagagc gacaagccga agaacatcca gccaagttga gtgatggtcg accaggccag
    23401 caaccgcttc atatcctgct gtggcaaata catcagaaat ccataaagaa tggtgaccag
    23461 ggccatgacc atgccaacgc cgccaatcac atgcgggata ttgccgccgt cgataatggc
    23521 gcgggcaaaa atgtaaacgc ccactttcac catcgatgcg gcgtggagat aggcgctgat
    23581 cggtgttggc gcttccattg cgtccggtag ccacgcttgc atcggtagct gggccgattt
    23641 cccccacgcg gcaaacagga tgccgccata aaccagataa cgtgcgtcgc cgtgtaactc
    23701 gctcatcgcg ctaagcgcaa acgttccggt ctgcaaaaac agcgtggcgg cggcaagata
    23761 caaccccaac gaaccgatat gggtgataag caacgctttt agtgctgaac gctgcgcttt
    23821 atcgctctga taataactga tcaacaccca ggagcagccg cccgtaattt caaaaaacaa
    23881 caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaccag
    23941 caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag
    24001 ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag
    24061 cgtacacacg cgatcaatga ccaggccaaa caatgccaca tcccccacgt tgaccaacgt
    24121 caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca acatgcacag
    24181 cgtggtcagc gcggcgaaca aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga
    24241 aacgaccagt gcgccgataa aaggcagcaa taacgtcgtc agagcaagat tttccatatt
    24301 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
    24361 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
    24421 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    24481 gcaggcaggc gagagaaagt tcttgcgcgc acccaaaggg cagaaacagg gccacaaaca
    24541 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgccagcgcc agcccggcac
    24601 cggaatattc tgtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    24661 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    24721 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    24781 ctgctatcag cgccaatacc agcagtgaga gaatgagcat tggttcgacc agaatgccga
    24841 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agcccggaaa
    24901 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    24961 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    25021 tgattaatac ccacggcatc aggcggaaca tcagacctga agatataggc gcaacttcct
    25081 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    25141 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagcggggtt agcatcagca
    25201 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    25261 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    25321 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    25381 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    25441 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    25501 agacgaaata gcgcagagaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    25561 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    25621 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    25681 aacagcaaca gtaatgaggg ggtgacctgt gtatgaaacg tgccgggtac aagtgctatt
    25741 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatatg cattatcttc
    25801 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    25861 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca
    25921 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    25981 gccagcatca ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    26041 agtgagaaca gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaagg
    26101 gctgatatcg cgaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    26161 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc gcccgcgccg
    26221 agaaatagca gccctttgaa cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    26281 ccaaccacgg tgagcagcgg gtcgtgtagt gacagaccga ccatcgccac accgactgcc
    26341 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    26401 tccgccagcg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    26461 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    26521 ccgaaaatac cgattttgac cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg
    26581 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    26641 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    26701 aaactggcaa aatctaggct gccgctttcg cgccccatca gcaagaaggc gatcataatc
    26761 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    26821 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    26881 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    26941 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    27001 agcgaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    27061 attcgcaggg ttaaatcggc aaagtcaaaa ggggtggcaa aacgggcaac taaagttacg
    27121 ccttcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    27181 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    27241 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    27301 cctgagtgag ggaaagggga tcctccctgc cattggcaaa ccagcttgcg gcaaggcgct
    27361 gtttttcttt catctgacgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    27421 ggcaagcgcg aacgcaggca ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    27481 cgacggtctg gacacccggt tcccagcgca gcaaagcatg ttgtgttggc gcgttttctt
    27541 cgccgtcttt gagtgagcct tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    27601 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    27661 tacagaggct ttcgttgagt tggatcgcat catccctctg ggagatggca ttgaccgggc
    27721 agacctgcac gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    27781 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    27841 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    27901 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcataatg cataaaggat
    27961 gccagtatga ttaatttaat atattcaata agttatgaat taactgaatt ctgaacggta
    28021 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    28081 aagtgagaat gtgatcttga tcacgataag tgaggtgtgc tgaccgcaag cgcagccagc
    28141 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    28201 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    28261 agctgatgcg atgaatacca tcgtaggttt tgcccatgaa ggacttttcc ccccagacgc
    28321 cgaattgctc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    28381 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    28441 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    28501 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    28561 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    28621 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    28681 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    28741 gtccgcagat gcggggctgt gggcagtaat ctgaatatgc aactgcgcag cccgttcatt
    28801 ttcagcaggt tgtgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    28861 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    28921 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    28981 ttcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    29041 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    29101 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    29161 caacgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    29221 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    29281 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    29341 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    29401 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    29461 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    29521 tgtggtgatg atgacgctgg agctggctga cgggcgcatt ccggtgattg ccgggaccgg
    29581 tgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    29641 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    29701 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    29761 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    29821 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    29881 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    29941 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    30001 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    30061 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    30121 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    30181 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    30241 tgatggctta ctctgttcat aagtcgcgcc tggcaacggt tgcgggtgtt tcgcttgttt
    30301 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    30361 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    30421 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    30481 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    30541 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    30601 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    30661 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    30721 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgctga
    30781 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    30841 agatgatgaa cgttatttcc gctggtctgg ataaatctgc cactgacgcc gcgaacgctg
    30901 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    30961 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    31021 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    31081 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    31141 ctggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    31201 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    31261 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    31321 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    31381 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    31441 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    31501 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    31561 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    31621 tcgcaaaggt atggtgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    31681 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    31741 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg
    31801 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    31861 cgacgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtaag
    31921 caaagagaac ctggcacgca tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    31981 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    32041 aggtgaagtg gtactgggcg atgagttctc cccggacggt agccgcctgt gggacaaaga
    32101 aacgctggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaggc
    32161 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    32221 ttgccgtgct gctggcacgg caagacaacc attctcgtaa gatgtgcatt gaacgctcat
    32281 tcctccgcca tttcatcccg ttttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    32341 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    32401 aatgttgaag acaggcgcaa tagctccggc ggtccgtcaa tgggcggtcc cggtttccgt
    32461 ctgccgagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    32521 ggcgttgatt taaccgggtt gatgaccggg cagccggttt ctcaacagca atcaacgcgg
    32581 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    32641 gaagacacct ggggacaaca gttcgagaag atgggtaaga cctatcagca accgaagctg
    32701 gtcatgtacc gtggaatgac gcgtaccgga tgcggggcgg gccagtccat aatggggccg
    32761 ttctattgcc cggcggatgg tacggtttat atcgatctct ccttctatga tgacatgaaa
    32821 gacaaacttg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    32881 catcatgtgc agaaactgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    32941 acgcaggcgg aagtgaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    33001 ggtgtctggg ggcatagcat gcagcagcaa ggcgttctgg aaaccggcga tctggaagag
    33061 gcgctgaacg cggcgcaggc catcggcgat gaccgtttac aacagcaaag tcaggggcga
    33121 gtagtaccag acagtttcac tcatggcact tctcagcaac gctacagctg gtttaaacgt
    33181 ggtttcgaca gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat ttaacttccg
    33241 gggcagggat ggctgaactg actgcgcttc acacattaac agcgcaaatg aaacgtgaag
    33301 ggatccgccg cttgctggtg ttgagcgggg aagagggttg gtgttttgat catgcgctta
    33361 agttacgtga tgccttacct ggcgactggc tgtggatttc gccgcagcca gatgctgaaa
    33421 accactgttc tccctcggca ctacaaactt tacttgggcg cgagttccgg catgcggtat
    33481 tcgacgcccg ccacggcttt gatgccgctg cctttgccgc acttagcgga acgttgaaag
    33541 cgggaagctg gctggttttg ttactccctg tatgggaaga gtgggaaaac caacctgatg
    33601 ccgactcgct gcgctggagt gattgccctg accctattgc gacgccgcat tttgtccagc
    33661 atttcaaacg cgtacttacg gcggataacg acgctatctt ctggcggcaa aaccagccgt
    33721 tctcgttggc gcattttact ccccgtactg actggcaccc cgcgaccggc gcaccacaac
    33781 cagaacaaca gcaaatctta cagcagctac tgaccatgcc gtcgggcgtg gcagcggtaa
    33841 ctgctgcgcg tgggcgcggt aaatcggcgc tggcagggca actcatttct cgtattgcgg
    33901 gtagtgcgat tatcactgcg cccgcaaaag cggcaacgga tgtactggca caatttgcgg
    33961 gcgagaagtt tcgctttatt gcgcctgatg ccttgttagc cagcgatgag caagccgact
    34021 ggctggtggt cgatgaagcc gcagccatac ctgcgccgtt gttgcatcaa ctggtatcgc
    34081 gttttcctcg aacgttgtta accactacgg tgcagggcta cgaaggtacc ggacgtggtt
    34141 ttttgctgaa attttgcgct cgctttccgc atttacaccg ttttgaactg caacagccga
    34201 tccgctgggc acagggatgc ccgctggaaa aaatggttag tgaggcactg gtttttaacg
    34261 atgaaaactt cacccataca ccacaaggca atatcgtcat ttccgcattt gaacaaacgt
    34321 tatggcgaag cgagccagaa acgccgttaa aggtttatca gttattgtct ggtgcgcact
    34381 accggacttc gccgctggat ttacgccgca tgatggatgc accagggcaa cattttttac
    34441 aggcggctgg cgaaaacgag attgccggag cgctgtggct ggtggatgag gggggattat
    34501 ctcaagaact cagtcaggcg gtatgggcag gttttcgtcg cccgcggggt aatctggtgg
    34561 cccagtcgct ggcggcgcac ggcagcaatc cactggcggc gacattgcgt ggacggcggg
    34621 tcagccggat agcagttcat ccggcgcgtc agcgcgaagg cgttgggcaa cagctcattg
    34681 ccagcgcttt gcaatatagg cctggcctcg actatctttc ggtgagtttt ggttacaccg
    34741 gggagttatg gcgtttctgg caacgctgcg gttttgtgct ggtgcgaatg ggtaatcatc
    34801 gtgaagccag cagcggttgc tatacggcga tggcgctgtt accgatgagt gatgcgggta
    34861 aacagctggc tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct ctcgcgcagt
    34921 ggaatggcga aacactccct gttgatccac taaacaatgc cgtcctttct gacgacgact
    34981 ggcttgaact ggccggtttt gctttcgctc atcgtccgct attaacatcg ttaggttgct
    35041 tattgcgtct gctacaaacc agtgaactgg cattaccggc gctgcgtggg cgtttacaga
    35101 aaaacgtcag cgacgcgcag ttatgtacca cacttaaact ttcaggccgc aagatgttac
    35161 tggtccgtca gcgggaagag gccgcacagg cgctgttcgc acttaatgat gttcgcactg
    35221 agcgtctgcg cgatcgcata acgcaatggc aattttttca ctgactcctt caattttctc
    35281 ccatggtcat tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga gttcgccatg
    35341 aaacatgacc attttgttgt tcaaagcccg gataaacctg cgcaacagtt gttactgctt
    35401 ttccacggtg tcggggataa cccggtggca atgggggaaa taggtagctg gtttgcaccg
    35461 ttgttcccgg atgcgctggt ggtgagtgtc ggtggcgcgg agccgagcgg taatccggcg
    35521 gggcgtcagt ggttttcggt gcagggtatt acggaagata atcgccaggc gcgtgtggat
    35581 gccatcatgc cgacgtttat tgaaacggtg cgctactggc agaaacagag cggagtaggg
    35641 gctaacgcca cggcgctaat cggtttctct caaggcgcga ttatggcgct ggagagcatt
    35701 aaagccgaac cgggccttgc ttcacgcgtc attgctttta acggacgcta tgcgagcctg
    35761 ccggaaaccg cgtctacagc cacgacaatt cacttgattc atggcggtga agatccggtt
    35821 atcgatctgg cgcatgcagt tgcggcgcag gaagcgttaa tcagtgccgg tggtgacgtg
    35881 acgctggata tcgtggagga tttaggccat gcgatcgaca atcgcagtat gcaatttgcc
    35941 ctggatcact tgcgttatac cattccgaaa cattatttcg acgaagcgct aagcggcggt
    36001 aagcctggcg atgatgatgt gattgaaatg atgtaataat agccgggtag gcctgataag
    36061 accgtatcag gcatcatggc cggatgcggc gttaacgcct tatccggcca cttagcttac
    36121 ttcttcggtt gatccttttt cggccagtcg tcgtcatcgt cccacttatc gttgaaatca
    36181 cgatgcgggg gaagctccgg cttgttggcg agaaatttct tatggtcgac gcgcttgaga
    36241 tctttaatca cgttcagcag gacgcctacc aaaaagacaa tcaccagaat ccaccaatat
    36301 ttagccagcc agtccatgct tatttcctct tgcagaacca ctcatcacgc gacgagctgt
    36361 tccatgatac gttgatacat acgggcaagt agctgcaggt cggcagcgtt cacacattca
    36421 ttaattttat gaatagtggc attgaccggc ccgagttcca ccacctgcgc ccccatgcgg
    36481 gcaataaagc gcccgtcgga cgttccgcct gtggtcagta gctgcggttt aatttcatta
    36541 tagtgctcaa ccgcgttaac gaccgcatcc accagtttac cgcgcgcggt caaaaatggc
    36601 tgcccggaaa gccaccaatc caccgtatag cgcagttgat gtttttcaag cagggcaagc
    36661 acctgcgctt tgatcatctc atcagtcagt tcggtgctga agcggaagtt aaactgcaca
    36721 aacagttcac ccggaataac gttgttactg cccgttcccg cctgaatatt ggcaatctgc
    36781 atactggtcg ccgggaagaa ttcattgccc tgatcccact caatagccac taattcatta
    36841 aggaaaggtg ctgcgcgatg taccggattg tcagccagat gtgggtaggc aacatgcccc
    36901 tgaacgccat gaatggttag gttgcaggtt aacgatccac gacgaccatt tttcaccaca
    36961 tcacctacca cttcgatact cgacggttcg ccgaccaggc agtaatcgag acgctcatta
    37021 cgtgccatta acgcttcgac ggcttttacc gtaccattgt gggcactggc ttcttcatca
    37081 gaggtgatca gaaatgccag tcgccctgta tggttgggat gttgtgcgac aaaacgttct
    37141 gcagctacca ccatcgccgc cagcgagcct ttcatatctg ccgcaccgcg cccgaataac
    37201 atgccgtcac gaatggttgg ttcaaacggc ggattgatcc aacgatcggc gtcgccaggc
    37261 ggcaccacgt cggtatgccc ggcaaaggcc aatgtttcac cttgcccacg ccatgcccaa
    37321 aaattctgcg tatcggcaaa gtccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt
    37381 tcaatcaaca aagcctggca tcctgcgtca tcaggactca gggaagggcg gcgaataagc
    37441 tgttgtgtca gctcaataac cgggcacgac atagactaca cctcatggaa aaatttctga
    37501 taactggaat cactgaaacc cagcagcata ggcttaccag gcgcgcagag caatggacgt
    37561 ttgataattg caggcatttc agtcattaat gccgccgcag aggccgcatc ggtgattata
    37621 ttgcgggtgg tttcgtccag tttacgccag gttgtaccac gggtgttgag taacgcttcc
    37681 cagcctaatt cgttgataaa accgttcaat aattcgctgt ccagcccatc gacgcggtaa
    37741 tcatgaaaac gatagtcgat gttattggct tccagccaac ggcgagcctt tttaatggtg
    37801 tcacaatttt tgatgccgta aagtgtaacc ataatgaatc ctttttcgat ttatgagaat
    37861 aaatactcat ttaagagtga aaacatt
//
LOCUS       DBFIFS010000024        37237 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.024, whole genome shotgun sequence.
ACCESSION   DBFIFS010000024 DBFIFS010000000
VERSION     DBFIFS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.024"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..386
                     /gene="ytfI"
                     /locus_tag="N7A07_001101"
     CDS             <1..386
                     /gene="ytfI"
                     /locus_tag="N7A07_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HGU2475806.1"
                     /translation="RQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLGFALKIYGGDER
                     FRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSDITKEKICEKY
                     KSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(449..1003)
                     /gene="ytfJ"
                     /locus_tag="N7A07_001102"
     CDS             complement(449..1003)
                     /gene="ytfJ"
                     /locus_tag="N7A07_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU2475807.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1328..1534
                     /gene="ytfK"
                     /locus_tag="N7A07_001103"
     CDS             1328..1534
                     /gene="ytfK"
                     /locus_tag="N7A07_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU2475808.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1613..2956)
                     /gene="paeA"
                     /locus_tag="N7A07_001104"
     CDS             complement(1613..2956)
                     /gene="paeA"
                     /locus_tag="N7A07_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU2475809.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3279..3917)
                     /gene="msrA"
                     /locus_tag="N7A07_001105"
     CDS             complement(3279..3917)
                     /gene="msrA"
                     /locus_tag="N7A07_001105"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU2475810.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4123..5856
                     /gene="tamA"
                     /locus_tag="N7A07_001106"
     CDS             4123..5856
                     /gene="tamA"
                     /locus_tag="N7A07_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU2475811.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5853..9632
                     /gene="tamB"
                     /locus_tag="N7A07_001107"
     CDS             5853..9632
                     /gene="tamB"
                     /locus_tag="N7A07_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU2475812.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9635..9976
                     /gene="ytfP"
                     /locus_tag="N7A07_001108"
     CDS             9635..9976
                     /gene="ytfP"
                     /locus_tag="N7A07_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU2475813.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10188..10439
                     /gene="chpS"
                     /locus_tag="N7A07_001109"
     CDS             10188..10439
                     /gene="chpS"
                     /locus_tag="N7A07_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU2475814.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10433..10783
                     /gene="chpB"
                     /locus_tag="N7A07_001110"
     CDS             10433..10783
                     /gene="chpB"
                     /locus_tag="N7A07_001110"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU2475815.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10863..11393)
                     /gene="ppa"
                     /locus_tag="N7A07_001111"
     CDS             complement(10863..11393)
                     /gene="ppa"
                     /locus_tag="N7A07_001111"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU2475816.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11703..12659
                     /gene="ytfQ"
                     /locus_tag="N7A07_001112"
     CDS             11703..12659
                     /gene="ytfQ"
                     /locus_tag="N7A07_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU2475817.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            12799..14301
                     /gene="ytfR"
                     /locus_tag="N7A07_001113"
                     /pseudo
     CDS             12799..14301
                     /gene="ytfR"
                     /locus_tag="N7A07_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
     gene            14315..15337
                     /gene="ytfT"
                     /locus_tag="N7A07_001114"
     CDS             14315..15337
                     /gene="ytfT"
                     /locus_tag="N7A07_001114"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU2475818.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15324..16319
                     /gene="yjfF"
                     /locus_tag="N7A07_001115"
     CDS             15324..16319
                     /gene="yjfF"
                     /locus_tag="N7A07_001115"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU2475819.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16352..17350)
                     /gene="fbp"
                     /locus_tag="N7A07_001116"
     CDS             complement(16352..17350)
                     /gene="fbp"
                     /locus_tag="N7A07_001116"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU2475820.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            17526..18899
                     /gene="mpl"
                     /locus_tag="N7A07_001117"
     CDS             17526..18899
                     /gene="mpl"
                     /locus_tag="N7A07_001117"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU2475821.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19055..19606)
                     /gene="yjgA"
                     /locus_tag="N7A07_001118"
     CDS             complement(19055..19606)
                     /gene="yjgA"
                     /locus_tag="N7A07_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU2475822.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19700..21052
                     /gene="pmbA"
                     /locus_tag="N7A07_001119"
     CDS             19700..21052
                     /gene="pmbA"
                     /locus_tag="N7A07_001119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU2475823.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21236..21622
                     /gene="cybC"
                     /locus_tag="N7A07_001120"
     CDS             21236..21622
                     /gene="cybC"
                     /locus_tag="N7A07_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU2475824.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(21667..22131)
                     /gene="nrdG"
                     /locus_tag="N7A07_001121"
     CDS             complement(21667..22131)
                     /gene="nrdG"
                     /locus_tag="N7A07_001121"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU2475825.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22321..24459)
                     /gene="nrdD"
                     /locus_tag="N7A07_001122"
     CDS             complement(22321..24459)
                     /gene="nrdD"
                     /locus_tag="N7A07_001122"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU2475826.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24853..26508)
                     /gene="treC"
                     /locus_tag="N7A07_001123"
     CDS             complement(24853..26508)
                     /gene="treC"
                     /locus_tag="N7A07_001123"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU2475827.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQLGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26558..27979)
                     /gene="treB"
                     /locus_tag="N7A07_001124"
     CDS             complement(26558..27979)
                     /gene="treB"
                     /locus_tag="N7A07_001124"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU2475828.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28098..29045)
                     /gene="treR"
                     /locus_tag="N7A07_001125"
     CDS             complement(28098..29045)
                     /gene="treR"
                     /locus_tag="N7A07_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU2475829.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29230..29283
                     /gene="mgtL"
                     /locus_tag="N7A07_001126"
     CDS             29230..29283
                     /gene="mgtL"
                     /locus_tag="N7A07_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU2475830.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29424..32120
                     /gene="mgtA"
                     /locus_tag="N7A07_001127"
     CDS             29424..32120
                     /gene="mgtA"
                     /locus_tag="N7A07_001127"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU2475831.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32326..32712)
                     /gene="ridA"
                     /locus_tag="N7A07_001128"
     CDS             complement(32326..32712)
                     /gene="ridA"
                     /locus_tag="N7A07_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU2475832.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32785..33246)
                     /gene="pyrI"
                     /locus_tag="N7A07_001129"
     CDS             complement(32785..33246)
                     /gene="pyrI"
                     /locus_tag="N7A07_001129"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU2475833.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33259..34194)
                     /gene="pyrB"
                     /locus_tag="N7A07_001130"
     CDS             complement(33259..34194)
                     /gene="pyrB"
                     /locus_tag="N7A07_001130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU2475834.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34198..34332)
                     /gene="pyrL"
                     /locus_tag="N7A07_001131"
     CDS             complement(34198..34332)
                     /gene="pyrL"
                     /locus_tag="N7A07_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU2475835.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34613..35008)
                     /gene="yjgH"
                     /locus_tag="N7A07_001132"
     CDS             complement(34613..35008)
                     /gene="yjgH"
                     /locus_tag="N7A07_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU2475836.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35139..35852)
                     /gene="bdcA"
                     /locus_tag="N7A07_001133"
     CDS             complement(35139..35852)
                     /gene="bdcA"
                     /locus_tag="N7A07_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2475837.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            35923..36516
                     /gene="bdcR"
                     /locus_tag="N7A07_001134"
     CDS             35923..36516
                     /gene="bdcR"
                     /locus_tag="N7A07_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU2475838.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            36661..37113
                     /gene="tabA"
                     /locus_tag="N7A07_001135"
     CDS             36661..37113
                     /gene="tabA"
                     /locus_tag="N7A07_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU2475839.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         8939 a         9283 c        10026 g         8989 t
ORIGIN      
        1 aacgacaagg gccaggaggt ggaattaaac agtgcgatgg atggtatatt tacgaaaaaa
       61 taattgatga taattttgcc atagtcatag aatctttggg ctttgcgctt aaaatctatg
      121 gaggggatga acgttttcgt aatggttcct ctgttgtctt ggaggatgaa gattactctc
      181 ttatttataa ttttctggtt aatgcgggtt gccaacaagt cgaattagcc gaacaagtgg
      241 atgcgatagt aagtgcgaat ctggccgcag atagtgatat taccaaagaa aagatttgcg
      301 agaaatataa atcgaccatt gaggcgttta aaaaagagca actagcgtta ccagtattag
      361 ttcggcgtaa gaactcagag acttaattcc cctatcccat agataacgat aggggaaaat
      421 tatttttgca gaatttatgt tattgccatt atttgagcaa cttttgcagc aggtccatca
      481 cttgctgcac ctcttcctga gtgagcgccc cgtctttggc ccattgcacg cgaccgtctt
      541 tatccagcac cactacagcg gaactttctt catccagctg ccaggcaccg cgcgcgacgc
      601 cattgctatc gacaataaac tgcgaccagg gataaagctt tttattactc tccagactgc
      661 tgcgcacaaa catgccggaa cccggaatcg cgtcgtcggt gttaacaatg gtggtggtct
      721 ggtaacgatc gtgcggtaac ttcgctgatt taatcgcttc aatcagcgtc gcgtttttct
      781 cttttgcaga ggtgcgacca gcaatatgtt gcagtactcg cacttttccc actaactgcg
      841 cgctgttcca ggttttgtag ctaaactgat ctttatcaag caccaactcg ccccgatcgg
      901 taatgccaat cggcggcact cgctgaccgg tttcgaactg atgtgcggaa gccatcatcg
      961 gcaacagcag gcaggtgagt gccagaatct tgcgtagggt catggtgttt ccttcttatg
     1021 atatgcaggt gatccgacca cttgggtctg agttttaatc atatgtgcta tttatcgaat
     1081 ttcccgcaag tgtgatgcca gtttgcggtc aagcgcacaa atcatatgaa aaatgaatgc
     1141 ttatactgaa gaccgcgctt cggtaaaaag ataattctga ataattgtaa cctttaggta
     1201 aaaaaagtta tacgcggtgg aaacattgcc cggatagtct atagtcacta agcattaaaa
     1261 tttgcgcctc ataatagttg ggccgattgt ggcaccgcac aggcgtaata ctcagcagga
     1321 gataacaatg aaaattttcc aacggtacaa cccacttcag gtggcgaagt acgtaaagat
     1381 cctgttccgt ggacgattat acatcaagga cgttggcgct tttgaattcg ataagggtaa
     1441 gattcttatc ccaaaagtga aggataagct gcatttgtca gtgatgtccg aagttaaccg
     1501 tcaggttatg cgtctgcaaa cagagatggc ttaaccaaag tgctatgcag taaaaagtgc
     1561 tatgcagtaa taagacggct cctgattcag gagccgttga tgtttatggg ggttacgcga
     1621 cgctttcttc tttatcttta gcgtcaggca gttttggcga aagggctgtg gccttgctgt
     1681 cgatccgggt caccagcagc tggtcgatgc ggtagttatc gatatccacc acttcaaatt
     1741 tgtagccggc gaatttcacc gaatcggtgc gtttcgggat cttacgcagc ataaacatca
     1801 taaagccgcc gatggtttcg tagttgcccg actgcgggaa ctcgtcaata tccagcacgc
     1861 gcatgacgtc gtcaatcgga gtgccgccgt caatcagcca tgagttctca tcacgggcga
     1921 caatctgctc ttccagtccc tgaccgacca gatcgcccat cagcgtggtc atcacgtcgt
     1981 tgagggtgat gatccccact accagcgcgt actcgttcat gatcaccgcg aagtcttctc
     2041 ctgcggtttt aaaactttcc aacgcctctg aaagggttaa cgtatccggc acaatcagcg
     2101 tgttgcgaat ttgtacgccg ctgttcagtg ccaggctttg gttagccagc acgcggttca
     2161 gcaggtcttt agaatcgaca tagccgatga tgtggtcaat atcttcatta cagacgagga
     2221 acttagagtg cggatgttcc gccaccttat ttttcagact ttgctcatct tcgtggagat
     2281 caaaccaaat cacgttttca cgcggtgtca ttgaagacgg aacggtacgg gattccagct
     2341 caaagacgtt ttcaatcagc tcgtgttcct gtttacgtaa cacgcccgcc agcgcaccgg
     2401 cttccactac cgcgtagatg tcatcagaag tgatgtcatc tttacgtacc attggcagtt
     2461 tgaaaatacg gaagattatg ttcgccaggc cgttgaagaa ccacaccagc ggggtgcaaa
     2521 cgtacaggca gaagcgcatc gggttgatga tacgcaaagc cacagcttct ggcgcaatca
     2581 taccgatgcg tttcggggtt aaatccgcaa acaggataaa catgccagtc actaacgaga
     2641 aagagagaat aaagctcagt tgctcagaga gctctgccga catatagcgg gagaacaggc
     2701 tgtgaaaagc tggagaaaat gccgcatcac cgacgatacc gccgagaatc gccactgcgt
     2761 tcagaccgat ttggaccacg gtaaagaaca tgccgggatt ttcctgcata ttcagaacgc
     2821 gttgggcatt tatattgcct tcatcagcca gcagtttaag tttgattttg cgtgaggcgg
     2881 caagtgagat ctcagacatc gagaagaacg cacttacagc gatcaagcag agtatgacta
     2941 aaatactgtt taacatatct tatccgacct ttcaggccag atcctcggaa ggggaagttg
     3001 attaatttgt gtgaaataca cattgaaagc cgattcgaag aaagtgaacc ggcattttca
     3061 gtggctagta tagcgtaagg tactgtaaag ccgccagagg gttaaaattc agacagctga
     3121 aaaatgcaaa actgcctgat gcgctatgcc gatcaggcct acgcagtcct tgcaatatat
     3181 tgaatttgga cgattttgta ggcctggcaa ggcgtttacg ccgcatccgg cataaacaaa
     3241 acgcacttta tcaacaatct acatttgtac ccgtaacgct atgcttccgg tggcagacag
     3301 acgccaattc cgccaattcc acagtaaccg tacgggtttt tatgcagata ttgctggtgg
     3361 tcatcttcgg cataataaaa cggtgtggcg ttagcgattt ccgtggtgat gtgacgatcg
     3421 tcatcggcgg caagcatcgc cgcctgaaaa cgttccagac tggcgcgagc tgcggcatcc
     3481 tgttctgggg tcagcggata aatcgctgaa cgatactgcg tgccgtggtc attgccctga
     3541 cgcattccct gggcgggatc gtgattctcc caaaatacct gtagcaactg ctcatagctg
     3601 atgacggaag gatcgtaaac aatgcgtacc gcttcggcat gaccagtatc accggagcac
     3661 acttcccgat aagtcggatt tggcgtatag ccgccggtat aacctgcggc ggtgctgtaa
     3721 acgccgtgta actgccagaa cagacgctcc acaccccaga aacaacccat cgcaaaaatg
     3781 gcaatctcca ttccgtcagg tacattggtc attgagtgac cgttaaccgc atgcagcgtg
     3841 gctacgggca tcggggtgtt acgtccaggc agggcatcgg cgggggaaac cagatgcttt
     3901 ttatcaaata aactcatggt gtcgctctcc cgaaaatcgg tcattggggt taaggttgta
     3961 acaagaggcg tatttgcaca caataaccac cgtgaatagg tctaaagtaa aacataagaa
     4021 atatttgggg tttagtctgc tttttaatcc atattactgg atttttgtta agccgtttaa
     4081 cggcgttcca ggggcaggaa aaaaggatat tcaggagaaa atgtgcgcta tatccgacag
     4141 ttatgctgtg taagcttact ctgcttaagc ggatctgccg tcgccgcgaa cgtccgtcta
     4201 caggtcgagg ggttatcggg acagctggaa aagaacgttc gtgcgcagct ttctacgatt
     4261 gaaagtgatg aagtgacgcc agaccgtcgc tttcgcgcac gcgtcgatga tgctatccgc
     4321 gaaggtctga aagcgctggg ttattaccag ccgaccattg aatttgatct ccgtccaccg
     4381 ccaaagaaag ggcggcaggt attgatcgcc aaagtcacgc caggcgtgcc ggtgttaatt
     4441 ggcggcaccg atgtggtatt gcgcgggggc gcgcggaccg ataaagacta tttgaaattg
     4501 ctcgatactc gcccggctat tggcacggta ctgaaccagg gcgattatga aaatttcaaa
     4561 aagtccttaa ccagcattgc gttgcgtaaa ggttatttcg atagcgaatt taccaaagcg
     4621 cagctgggca ttgcgctcgg cctgcataaa gccttctggg atattgatta taacagtggc
     4681 gaacgttacc gctttgggca tgtgaccttt gaaggatcac aaatccgcga tgaatacctg
     4741 caaaatctgg tgccgtttaa agagggcgat gagtacgaat cgaaagatct ggcagaactg
     4801 aaccgccgac tttctgctac cggctggttt aactcggtgg tggtggctcc acaatttgat
     4861 aaagcgcgcg aaacgaaagt attaccattg acgggcgtgg tttcgccgcg aacagaaaac
     4921 actatcgaaa ccggggtcgg ttactctacg gacgtgggac cgcgcgtgaa agcgacgtgg
     4981 aaaaaaccgt ggatgaactc atacggtcac agtctgacca ccagtaccag tatttccgcg
     5041 ccggaacaga ccctcgactt cagctataaa atgccgctgc tgaagaatcc actggaacaa
     5101 tattatttgg tgcagggcgg ttttaagcgc actgacctga acgataccga gtctgactcc
     5161 actacgctgg tggcttctcg ctactgggat ctctccagcg gctggcagcg tgccattaac
     5221 ctgcgctgga gtctcgacca ctttactcag ggtgaaatta ccaacaccac gatgctgttt
     5281 tatcctgggg tgatgattag ccgcacgcgt tctcgtggtg gcctgatgcc aacctggggc
     5341 gactcgcaac gctactctat cgactactcc aacacggcct ggggttcaga tgtcgatttc
     5401 tccgttttcc aggcgcagaa cgtctggatc cgcacactgt acgatcgcca tcgttttgtg
     5461 acacgcggca cgctgggctg gattgaaacc ggtgatttcg acaaagtacc gccggatctg
     5521 cgtttcttcg ccgggggcga tcgcagtatt cgcggctaca aatacaaatc tatcgccccg
     5581 aaatacgcta acggtgacct gaaaggggcc tcgaagttga taaccggatc gctggaatac
     5641 cagtacaacg tgaccggaaa atggtggggc gcggtgtttg tcgatagtgg cgaagcggta
     5701 agcgatattc gccgcagcga ctttaaaacc ggtaccgggg tcggcgtacg ctgggaatcg
     5761 ccggtcgggc caatcaaact cgattttgcc gtaccggtcg cggataaaga cgaacacggg
     5821 ttacagtttt acatcggtct ggggccagaa ttatgagttt atggaaaaaa atcagcctcg
     5881 gcgtggttat cgttatctta ctgttgctgg gatcggtggc gtttctggtg ggcaccacca
     5941 gcggcctgca tctggtattt aaagcggcgg atcgctgggt gccaggactg gatattggca
     6001 aggtcaccgg cggctggcgc gatctcacct tgtctgacgt tcgttatgag cagccaggcg
     6061 tggcggtaaa agcgggtaat ctgcatctgg cggtcggtct tgagtgcctg tggaacagca
     6121 gtgtttgtat taatgacttg gcgctgaaag acattcaggt caacatcgac agtaaaaaaa
     6181 tgcctccttc tgaacaggtt gaagaagagg aagatagcgg tccgctggat ctctccacgc
     6241 cgtatcccat caccctgaca cgggtggcac tggacaacgt caacatcaag attgatgaca
     6301 ccacggtatc ggtgatggac ttcacctccg gcctgaactg gcaggagaaa accctgaccc
     6361 tgaaaccgac gtcgctgaaa ggcctgctga ttgctctgcc gaaagtggcg gaagtggcgc
     6421 aggaagaagt ggtcgaaccg aaaattgaaa atccgcagcc ggaggaaaag cctctcggcg
     6481 aaacactcaa agatctcttt tctcgcccgg tattgccgga aatgaccgac gtgcatctgc
     6541 cactcaacct gaacattgaa gagtttaagg gagagcaact gcgcgtgacg ggcgatacgg
     6601 acattaccgt gcgcaccatg ctgctgaaag tgagcagcat tgacggcaat actaaactgg
     6661 acgccctgga tatcgattcc aatcaaggga tcctcaacgc cagcggcacg gcgcagctgt
     6721 cagacaactg gccggtggat atcaccctca acagcacact gaacgtggag ccgttgaaag
     6781 gtgaaaaggt gaagctgaaa gtgggcggcg cgctgcgcga acagctggag attggcgtta
     6841 atctttccgg tccggtggat atggatttac gcgcccagac gcgactggcg gaagccggat
     6901 tgccgctcaa cgtggaagtg aacagcaaac agatttactg gccgttcact ggtgagaagc
     6961 agtatcaggc ggatgatctg aaactgaaac ttaccggcaa aatgaccgat tacacgctct
     7021 ctatgcgtac ggcagtgaag ggactggaga tcccgccagc caccattacc cttgatgcca
     7081 aaggtaatga acagcaggtc aatctcgaca aactcaccgt cgcggcgctg gaagggaaaa
     7141 ctgaactcaa ggcgttgctt gactggcagc aggccattag ttggcgcggt gagctaacgc
     7201 ttaacggcat taacaccgcc aaagagatcc cagagtggcc gtcgaaactc aatggcttga
     7261 ttaaaacccg cggtagcctg tacggcggca cctggcagat ggaggtgcca gaactgaagc
     7321 ttaccggtaa cgtcaaacag aacaaagtga acgttgacgg cacgctgaaa ggcaacagtt
     7381 atatgcagtg gatgatccca gggcttcatc tggaactggg gccaaacagt gccgaagtga
     7441 aaggcgagct gggggtaaaa gatctcaatc ttgatgccac catcaacgcg ccggggctgg
     7501 ataacgcgct gccggggctt ggcggtacag cgaaagggct ggtgaaagtt cgcggcacgg
     7561 tggaagcgcc acaactactg gcggacatca ccgcgcgcgg cctgcgctgg caggagctaa
     7621 ccgtggcgca ggttcgcgtg gaaggcgata tcaaatccac cgatcagatt gcgggtaaac
     7681 tcgacgtgcg cgttgagcaa atttcacagc cggatgtaaa tatcaacctc gtcaccctga
     7741 atgccaaagg cagcgaaaaa cagcacgagt tacagttgcg gattcagggc gagccggttt
     7801 ccgggcagct taatctggca ggaagttttg atcgcaaaga agaacgctgg aagggaactc
     7861 ttagcaatac ccgcttccag acgctggtcg gcccgtggtc gctgacccgc gatattgcgc
     7921 tggattaccg caataaggag caaaaaatca gcatcgggcc acactgctgg cttaacccga
     7981 atgcggaact gtgcgtgccg caaactatcg atgcgggggc cgaagggcgt gcggtggtga
     8041 atctcaaccg cttcgacctt gccatgctga aaccgtttat gccagaaacc actcaggcca
     8101 gcggtatctt cacgggtaaa gcggacgttg cctgggacac caccgaagag gggctgccgc
     8161 agggcagtat caccctttcg gggcgtaacg tgcaggtaac gcaaaccgtc aacgatgcgg
     8221 cgctgcccgt ggcgtttcag acactgaatc tgacggcgga attgcgtaac aaccgtgccg
     8281 aattgggctg gaccatccgc ctgaccaata acggccagtt tgatggacag gtgcaggtga
     8341 ccgatccgca aggccgccgt aatcttggtg gcaacgtcaa tatccgtaac ttcaaccttg
     8401 cgatgataaa ccccatcttt acccgtgggg aaaaagcagc ggggatggtg agtgccaact
     8461 tgcgtctggg tggtgatgtg caaagcccgc agttgtttgg tcagcttcag gttacgggtg
     8521 tggatatcga tggcaacttt atgccgtttg atatgcaacc gagccagctt gcggtcaact
     8581 ttaacggtat gcgctcgacg cttgccggta cagtacgaac ccagcagggt gaaatctacc
     8641 tgaacggtga tgccgactgg agccaaattg aaaactggcg ggcgcgggta acggcgaaag
     8701 gcagtaaagt gcggatcacc gtgccgccga tggtacgaat ggatgtatcg ccagatgttg
     8761 tattcgaggc tacaccaaac ctgtttaccc tcgatggtcg cgtggatgtc ccttgggcgc
     8821 gcatcgtggt gcacgatctg ccggaaagcg cagtaggcgt ctccagcgat gtggtgatgc
     8881 ttaacgataa cctgcaaccg gaagagccga aaacggcgtc gattccgatt aacagtaacc
     8941 tgattgtcca cgttggcaac aatgtgcgca ttgacgcctt tggcctgaaa gcgcggctga
     9001 cgggcgatct taacgtcgtt caggacaaac aagggctggg cctgaacggg cagatcaaca
     9061 tccctgaagg gcgcttccat gcctatggtc aggatctgat tgtgcgtaaa ggtgagttac
     9121 tgttctctgg cccgccggat caaccgtatc ttaatattga agctattcgt aacccggatg
     9181 ctacagaaga cgacgtaatc gccggagttc gcgtcactgg tctggcggac gaaccgaaag
     9241 cggagatctt ctctgacccg gcgatgtcgc aacaagctgc cttgtcttat ttgctacgtg
     9301 gacaagggct ggagagcgat cagagcgaca gtgcggcaat gacctcgatg ctgattggtc
     9361 tgggggttgc gcaaagtggc cagattgtag gtaaaatcgg cgagacgttt ggcgtaagca
     9421 atttagcgct cgacacccag ggagtaggcg actcctccca ggtagtggtc agcggctatg
     9481 tattgccagg tctgcaagtg aaatacggcg tgggtatatt tgactctata gcaacactca
     9541 cgttacgtta tcgcctgatg cctaagctat atctggaagc cgtgtctggt gtagaccagg
     9601 cactggattt gctctatcag ttcgagtttt agcaatgcga atatttgtct acggcagttt
     9661 acgccacaaa caaggcaaca gtcactggat gaccaatgcc cagttactgg gcgatttcag
     9721 tatcgataac taccagttgt atagcctggg ccactatcca ggcgcagttc cggggaacgg
     9781 aacggtacac ggtgaagttt atcgtattga caacgccacg ctggccgaac ttgatgcctt
     9841 gcgcaccagg ggcggtgaat acgcgcgcca gttgattcag acgccgtacg ggagtgcatg
     9901 gatgtacgtt tatcaacgac ccgtcgatgg attaaagcta attgaaagcg gcgactggtt
     9961 agacagggat aagtaaccct atgcatacgc caccttcggg tggcgttgtt ttttgcgaga
    10021 cgactcgcat tctgttttgt aattccctca ccttttgctt ttctctccga gccgctttcc
    10081 atatctatta acgcataaaa aactctgctg gcattcacaa atgcgcaggg gtaaaacgtt
    10141 tcctgtagca ccgtgagtta tactttgtat aacttaagga ggtgcagatg cgtattacca
    10201 taaaaagatg ggggaacagt gcaggtatgg tcattcccaa tatcgtaatg aaagaactta
    10261 acttacagcc ggggcagagc gtggaagtgc aggtgagcaa caaccaactg attctgacac
    10321 ccatctccag gcgctactcg cttgatgaac tgctggcaca gtgtgacatg aacgccgcgg
    10381 aacttagcga gcaggatgtc tggggtaaat ccacccctgc gggtgacgaa atatggtaaa
    10441 gaaaagtgaa tttgaacggg gagacattgt gctggttggc tttgatccag caagcggcca
    10501 tgaacagcaa ggtgctggtc gacctgcgct tgtgctctcc gttcaagcct ttaatcaact
    10561 gggaatgacg ctggtggccc ccattacgca gggtggaaat tttgcccgtt atgccggatt
    10621 tagcgttcct ttacattgcg aagaaggcga tgtgcacggc gtggtgctgg ttaatcaggt
    10681 gcggatgatg gatctacgcg cccggctggc aaagcgtatt ggtctggctg cggatgaggt
    10741 ggtggaagag gcgttattac gcttgcaggc ggtggtggaa taaggtgtgt ttatttatcg
    10801 cgggcataaa aaaaccctta ctaaccgaag cccggcgttc agggttatta cgccagaaga
    10861 acttatttat tctttgcgcg ctcgaaggag gcaacgattt cagctttagc ggcttctgcg
    10921 ttttcccaac cttcaacttt cacccacttg cctttttcga ggtctttgta gtgctcgaag
    10981 aagtgagcga tttgcgcttt cagcagttca ggcagatcgt taacgtcttt aatgtgatcg
    11041 tattctttgc tcagcttgct gtgcggaacc gcaaccagtt tcgcatcttc accggcttcg
    11101 tcggtcattt tcagaacgcc aaccggacgg caacggatca cagaacccgg ctgcagcggg
    11161 tacggagttg ggaccagtac gtcaactggg tcaccgtcca gagacagggt gtggttgatg
    11221 taaccgtagt tgcacggata gaacatcgcg gtggacatga agcggtcaac gaacagtgcg
    11281 ccgctctctt tgtcgatttc gtatttgatc ggatctgcgt tagccgggat ctcaataaca
    11341 acgtagatgt cttccggcag atctttaccc gcagggacgt tgagtaagct catgtctgtt
    11401 tcctttaaaa atatgtggca aacaagtgcc gagtattata gccaactcgc gccgaatgtc
    11461 ttcgcttgtt ttcgtcttca ttttcctttt tcgccagttt taagacggta tgcagagcag
    11521 gaaaatccat aaccgtagcc gcatttttca tagtgagatg aaagcgatta caaacttgtg
    11581 attaacgttt tatttacttt tttgaagtgt gatgtaacgc aatccgttac ataacgaatt
    11641 gtctatagtt ttttcgcgaa catcttttca ccaataataa ttactccgac gaggacaacc
    11701 ctatgtggaa acgcttactt gtagtctctg cagtctcggc agccatgtcg tctatggcgt
    11761 tggccgctcc attaaccgta ggattttcgc aggtcggatc ggaatccggc tggcgcgccg
    11821 cagaaaccaa tgtggcgaaa agtgaggccg aaaaacgcgg aattacgctg aaaattgccg
    11881 atggtcagca aaagcaggaa aaccagatta aagcggtacg ttccttcgtt gcacaagggg
    11941 tggatgcgat ctttatcgct ccggtggtcg cgacaggttg ggaaccggta ttaaaagagg
    12001 cgaaagatgc cgaaatcccg gtcttcttgc tcgaccgttc catcgatgtg aaagacaaat
    12061 ctctctatat gaccaccgtt actgccgaca acatcctcga aggtaagttg atcggtgact
    12121 ggctggtaaa agaagtgaat ggcaaaccat gcaacgtggt ggagctgcag ggcaccgttg
    12181 gggccagcgt cgccattgac cgtaagaaag gctttgccga agccattaag aatgcgccaa
    12241 atatcaaaat catccgctcg cagtcaggtg acttcacccg cagtaaaggc aaagaagtca
    12301 tggagagctt tatcaaagcg gaaaacaacg gcaaaaacat ctgcatggtt tacgcccata
    12361 acgacgacat ggtgattggt gcaattcagg caattaaaga agcgggcctg aaaccgggca
    12421 aagatatcct cacgggttcc attgacggtg taccggacat ctacaaagcg atgatggatg
    12481 gcgaagcgaa cgccagtgtt gaactgacgc cgaacatggc gggacccgct ttcgatgcgc
    12541 tggagaaata caaaaaagac ggcaccatgc ctgaaaagct gacgttaacc aaatccaccc
    12601 tttacctgcc tgataccgca aaagaagagt tagagaagaa gaaaaatatg gggtattgag
    12661 ggttgctatg cctgatgccg attcgtaggc cggataaggc gctcgcgccg catccggcga
    12721 tggtgcactg aagcctgatg cgacgcttac cgcgtcttat catgcctact gggagcacgc
    12781 tttacaccgg gggaaaccat gacgaccgac caacaccagg agatcctccg caccgaagga
    12841 ttaagtaaat ttttccccgg cgtcaaagcg ttagacaacg ttgatttcag cctgcgccgt
    12901 ggcgaaatca tggcgctgct cggtgaaaac ggggcgggaa aatcaacgct aatcaaagca
    12961 ttaactggtg tataccacgc tgatcgcggc accatctggc tggaaggcca ggctatctca
    13021 ccgaaaaata ccgcccacgc gcaacaactc ggcattggca ccgtctatca ggaagtcaac
    13081 ctgctaccca atatgtcggt cgctgataat ctatttatag gccgcgaacc caaacgcttc
    13141 ggccttctac gccgcaaaga gatggaaaag cgcgccaccg aactgatggc atcttacggt
    13201 ttctccctcg acgtgcgcga accgctcaac cgcttttcag tcgcgatgca gcaaatcgtc
    13261 gctatttgcc gggctatcga tctctctgcc aaagtgctga tcctcgatga acccaccgcc
    13321 agtctcgaca cccaggaagt ggagttactg tttgacctga tgcgtcagtt gcgcgatcgc
    13381 ggcgtcagcc tgatctttgt cactcacttt ctcgatcagg tctatcaggt cagcgatcgg
    13441 atcaccgtct tacgcaacgg cagtttcgta ggctgtcggg aaacgtgcta gctaccgcag
    13501 atcgaactgg taaaaatgat gctggggcgc gagctggata cccacgcgct acagcgtgcc
    13561 gggcgaacat tgttgagcga caaacccgtt gccgcgttca aaaattacgg caaaaaagga
    13621 acgatcgcac cgtttgatct cgaagtacgc cccggcgaga tcgtcggtct ggctggattg
    13681 ctgggatcag gacgtaccga aaccgccgaa gtgatcttcg gtatcaaacc tgctgacagc
    13741 ggcacggcgt tgatcaaagg caaaccgcaa aacctgcgat cgccacatca ggcttcggta
    13801 cttggcattg gcttctgccc ggaagacagg aaaaccgatg gcatcatcgc tgccgcctcg
    13861 gtgcgggaaa atatcatcct cgctctctag gcccagcgcg gctggctacg tcccatttcc
    13921 cgcaaagaac agcaagagat tgccgaacgc tttatccgcc agcttggcat tcgcacacct
    13981 tcaactgaac aaccgattga atttctctcc ggcggcaatc agcaaaaagt gttgctttca
    14041 cgttggctac tgacccgacc gcaatttctg atcctcgatg agccaacccg cggcattgat
    14101 gttggtgccc acgccgagat catccgcctg attgaaacgc tatgcgccga tggtctggcg
    14161 ctgctggtga tctcctccga actggaagaa ctggtgggct atgccgaccg ggtgatcatc
    14221 atgcgcgatc gcaaacaggt ggcggagatc ccgctggcag agctttccgt tccggcgatc
    14281 atgaatgcca ttgcggcgta aggagaacag tgtgatgcct caatctctcc cggacactac
    14341 gccgccaaaa aggcgctttc gctggccaac gggaatgccg cagctggcgg cactgttgct
    14401 ggtgctgctg gtcgatagcc tggtggcccc gcatttctgg caggtggtgc ttcaggatgg
    14461 gcgtttgttc ggtagcccta tcgacattct taatcgcgcc gcccccgttg cgctactggc
    14521 gattggtatg acgctggtga tcgcaacagg tgggattgat ctctccgtag gggcggtgat
    14581 ggctatcgcc ggagccacaa cggctgcgat gacggtcgcg ggattcagcc tgccgattgt
    14641 tttgttaagc gccctgggca ccggcatcct ggcgggattg tggaacggca tactggtagc
    14701 gatcctcaaa attcagccgt ttgtcgccac tctgatcctg atggtcgccg ggcgcggcgt
    14761 ggcgcaactg atcacctccg gacagatcat cacgtttaac tcgccggatc tctcatggtt
    14821 cggcagtgga tcgctgttgt tcctgccaac gccggtcatt atcgcggtgc tgacgcttat
    14881 cctgttctgg ctgttgaccc gcaaaacggc gctggggatg tttatcgaag ccgttggtat
    14941 caacattcgg gcggcaaaaa atgccggggt aaacacgcgg atcatcgtca tgcttaccta
    15001 cgtgttgagc gggctgtgtg cggcgattgc gggcattatc gtggcggcgg atattcgcgg
    15061 tgccgatgcc aacaacgccg gattatggct ggagctggac gccattctcg cggtggtgat
    15121 tggcggtggt tcgctgatgg gcggacgttt taacctactg ctttcggtgg tgggggcgct
    15181 gattattcag gggatgaaca ccggaatttt gctttcgggc tttccgccag agatgaacca
    15241 ggtggtgaaa gcggtggtgg tgctttgcgt gctgattgtc cagtcgcaac gctttatcag
    15301 tctgattaaa ggagtgcgta gccgtgataa aacgtaattt gccactgatg atcaccatcg
    15361 gcgtctttgt gctgggttat ctctactgcc tgacccagtt tcccggtttt gcttccacaa
    15421 gagtgatctg caatatcctg accgataatg cctttcttgg gataatcgcc gttggcatga
    15481 cctttgtgat cctctccggt gggatcgatc tctccgttgg ttcggtgatc gcctttaccg
    15541 gcgtgtttct ggcaaaagtg attggcgatt tcggcctctc gccgttgctg gcatttccgc
    15601 tggtgctggt aatgggctgt gcctttggcg catttatggg gcttttgatc gacgccctga
    15661 agatcccggc atttatcatt actttggcag gaatgttctt tttgcgcggc gtcagctacc
    15721 tcgtttcgga agagtcgatc ccgataaacc atcctgttta tgacacgctc tcaagccttg
    15781 catggaaaat tcctggcggc ggtcgcttaa gtgcgatggg gctgctgatg ctggcggtgg
    15841 tggttatcgg catattcctc gcgcatcgta cccgctttgg taatcaggtg tacgccattg
    15901 gcggcaatgc gacgtcggca aacctgatgg ggatttccac tcgcagcaca actattcgca
    15961 tttatatgct ctctaccgga ctggcgacac tggcggggat tgtcttctcg atttataccc
    16021 aggccggata cgcgctggcg ggcgtaggtg tggaactgga cgctatcgcc tcagtggtga
    16081 ttggcggtac gcttttgagc ggtggcgttg gaacggtatt agggacgctt tttggcgtgg
    16141 cgattcaggg actgattcag acttacataa actttgatgg tacgctgagc tcatggtgga
    16201 cgaaaatcgc catcggtatt ttgttgttta tttttatagc attacagcgt ggattaacgg
    16261 tgctgtggga gaatcgtcag agttcgcctg tgacaagagt caacattgcg cagcaataaa
    16321 atcgcctctc cgtgtggaga ggcgcaggag attacgcgtc cgggaactca cagataaagc
    16381 gttcgacatc ttcaaccata tggtcgttgc cgacaaagaa tgaacggcgc tggtgcaggg
    16441 tttccgggat aatatccaga atacgctctt tgccatcgct cgctttaccg cccgcttgtt
    16501 ccgccaggaa tgccatcggg ttgcactcat acagcaaacg cagtttgccg tccgggtggc
    16561 tggcggtgct tgggtagaga taaataccgc ctttcagtag gttacggtgg aaatccgcaa
    16621 ccagtgagcc gatataacgt gaggtatacg ggcggttggt ggatttatct tcttcctggc
    16681 agaatttaat gtacttcttc accccgttcg gaaacttaat gtagtttcct tcgttgatgg
    16741 agtaggtttt gcctttctcc gggaagcgca tccgttcctg gcacaggcag aaaacgccga
    16801 gcgaaggatc gtaagtaaaa gcgtgaacac cgcatccggt actgtaaacc agcatggtag
    16861 aggagccgta taccacgtaa cctgccgcaa cctgtttgtt accaggctgg aggaaatctt
    16921 cttccgttac cggcgtgccg acgggcgtaa ctcggcggta gatggagaaa atggtaccga
    16981 cagagacgtt aacatcgatg ttggacgagc catccagtgg gtccatcagc accacgtatt
    17041 ttgcatgttc acagccttca aagacaacaa tctcatcttc ttcttcagag gcaatgcctg
    17101 caacgatatc gcgtgctttc agtgcggctt tcagtttttc attagcgaac aagtcgagtt
    17161 tttgctgaac ctcgccctgc acgttctcag caccgctggc acccaggata tcaaccagtc
    17221 ctgctttgtt gatatcgcga tggataatct tggcgcccag ttttattgcc gacagcaaag
    17281 cagtgagctc accggtagca tgagaaaact cgtgctgctt ttcgacaata aattcaccta
    17341 acgttttcat aaaactttcc ctgcaatgtt tatggagtaa agcgaccgca acaatcttaa
    17401 caaataatct caatgttgcg ctcaggtgaa tcgcgccagc aaattacgga ttatcctgaa
    17461 atgcgtttct cacttgcccg acatatgcgt aaaatgagcg gcagattaaa aaaggatagt
    17521 gacgcatgcg cattcatatt ttaggaattt gtggcacgtt tatgggcggt ctggcgatgc
    17581 tggcgcgcca gttaggccat gaagtaacgg gttcggacgc caatgtgtat ccgccgatga
    17641 gcaccttact tgagaagcaa ggcattgagc tgattcaggg ttacgatgcc agccagctcg
    17701 acccgcagcc ggatctggtg attattggca acgccatgac ccgtggaaat ccgtgtgtgg
    17761 aagcggtact ggaaaaaaac atcccttata tgtcaggtcc acagtggctg cacgattttg
    17821 tgctgcgcga ccgctgggtg ctggccgttg ccggtacaca cggcaaaacc accaccgcgg
    17881 gaatggcgac ttggattctg gaacagtgtg gttacaaacc gggctttgta atcggcggtg
    17941 tgccggggaa ctttgaggtt tcggcgcgtc tgggcgaaag cgacttcttt gttatcgaag
    18001 cggatgagta cgactgcgcc ttcttcgaca aacgctctaa atttgtccat tactgcccgc
    18061 gtacgctgat cctcaacaac cttgagttcg atcacgccga tatctttgac gacctgaaag
    18121 cgatccagaa acagttccac catctggtgc gtatcgttcc ggggcagggt cgtattatct
    18181 ggccggaaaa tgacatcaac ctgaaacaga ccatggcgat gggctgctgg agcgagcagg
    18241 agctggtggg tgagcagggt cactggcagg cgaaaaagct gaccaccgat gcttccgaat
    18301 gggaagtttt gctggatggc gaaaaagtgg gcgaagtgaa atggtcgctg gtaggcgaac
    18361 ataatatgca caatggcctg atggcgattg cagcggctcg ccatgttggt gtagcgccgg
    18421 cagatgccgc taacgcgctg ggttcgttta ttaacgctcg ccgccgtctg gagttgcgtg
    18481 gtgaagcgaa tggcgtcacg gtatatgacg attttgccca tcatccgacg gcgattctgg
    18541 caacgctggc ggcgctgcgt ggcaaagttg gtggtacggc gcgcattatt gctgtgctgg
    18601 agccgcgctc gaataccatg aaaatgggga tctgcaaaga cgatctggca ccttcattag
    18661 gtcgtgccga tgaagtcttc ctgctgcaac cggcgcatat tccgtggcag gtggcagaag
    18721 tggcagaagc ctgcgttcag cctgcacact ggagtggcga tgtggatacg ctggcagata
    18781 tggtggtgaa aaccgctcag cctggcgacc atattctggt gatgagcaac ggcggttttg
    18841 gtgggatcca tcagaaacta ctggatggtc tggcgaagaa ggcggaagct gcgcagtaat
    18901 tcggcctcag cctgagatag tgtgcgtagg ccggataagg cgttcacgcc gcatccggca
    18961 tttgagcata gtgcctgatg cgacgcttga tgcgtcttat caggcctaca ggtgcaccgc
    19021 atccggcaaa ccatactcac atcaacaacg aaaattaccc ttcattctcc gccagttcgc
    19081 gcagatactg gaaaatctgg cgtgcggatt tcggcggctt attcccttct ttctctttct
    19141 tcgcgttgcg gatcagagta cgcagttgct gacgatcggc atcgggccac agatttaata
    19201 cctcagcgat ggcgtcatca ccttgatcga tcagacgatc gcgcaggttt tccagtttat
    19261 ggaacagcac cacctgctgg ttgtgacggt ttttcagctt atccagcgcc tgacggatcg
    19321 gttctacatc gcgctggcgc agcatcttac cgatgagctg caactggcgg cggcgacctt
    19381 ccatcttaat acgctgggcg agctcaatag ccgcgcgcag atccgcatct aacgggatct
    19441 tatccagcgc gtttttcccc agatcaacaa tttccgcgcc aaggcgtttt agctcctcgg
    19501 catcacgttt aatttcactt ttactgaccc agataatttc atcgtcttcg tcttcgatgt
    19561 catcaccggg aacgtcgtcg agccagtctt cgggctgctt agtcatctca ggctccttaa
    19621 aaaaagaggc taatgttacc agttaagatg cgcactgaaa aacggttctc tgttagactt
    19681 cagagaaact ctctacatta tggcacttgc aatgaaagta atctctcaag ttgaagcgca
    19741 gcgcaagatt ctggaagaag cagtttccac tgcgctggag ttggcctcag gcaaatcgga
    19801 cggtgcggaa gttgccgtca gcaagaccac cggcattagc gtaagcacgc gttatggtga
    19861 agtggagaat gttgaattca atagcgatgg cgcgctgggg atcactgttt atcaccagaa
    19921 ccgcaaaggt agcgcatcat ccaccgattt aagcccgcag gccattgccc gtactgtaca
    19981 ggcggcgctg gatattgccc gttatacctc gccagatccc tgtgccggcg tggcagacaa
    20041 agagctgctg gcctttgacg caccggatct cgacttgttc caccctgcgg aagtttcccc
    20101 ggatgaagcc attgaactgg cggcccgcgc agaacaggcg gcattgcagg cggacaaacg
    20161 catcaccaat accgaaggtg gcagctttaa cagccactac ggtgtcaaag tttttggcaa
    20221 cagccacggc atgttgcagg gttactgctc aacgcgtcat tcgctctcca gctgtgtaat
    20281 tgccgaagaa aatggcgata tggagcgtga ttacgcctac accattggcc gcgcgatgag
    20341 cgatttgcag acgccagagt gggtcggggc cgattgcgct cgccgtactt tatcgcgtct
    20401 gtcaccgcgt aaactctcca ccatgaaagc gccggtcatt tttgccaatg aagtggcaac
    20461 cgggcttttt gggcatctgg tgggggcgat agcgggtgga tcggtttacc gtaaatctac
    20521 cttcctgctg gattcgctgg gtaaacaaat tctgccggac tggctgacca ttgaagagca
    20581 tccgcatctg ctgaaagggc tggcatcgac gccatttgac agcgaaggtg tgcgcaccga
    20641 gcgtcgcgat attattaaag atggcatcct gactcagtgg ctgctgacca gctactcggc
    20701 gcggaaactg gggctgaaaa gcaccggaca tgcgggcggt attcacaact ggcggattgc
    20761 cggacaaggt ctaagcttcg agcagatgct caaagagatg ggcaccgggc tggtggtgac
    20821 ggaattgatg ggccagggcg tgagtgcaat taccggtgat tattcccgtg gtgcggcggg
    20881 cttctgggta gaaaacggcg aaattcagta tccggttagc gaaatcacca tcgcaggtaa
    20941 tttaaaagat atgtggcgca atattgtcac cgtcggtaac gatattgaaa cacgcagtaa
    21001 tatacagtgt ggttctgtgc tgttgccgga gatgaaaatc gccggacagt aaaaaaactg
    21061 gcgcgacctg ccgcgctata aactaaaaaa ttacacaaaa tcattcgcac tgcatcgaga
    21121 cggaaagtga atgagggccg gggagcgtac tcgcagtacg tgacccggtc gaatgagcgt
    21181 agtcgtcgat gaggcagtgt gaaggatgaa gtgtaaataa aaaaggaagt gagcaatgcg
    21241 aaaaagcctg ttagctattc ttgcagtctc ctcgttggta ttcagttcgg cgtcgtttgc
    21301 tgctgatctt gaagacaata tggaaaccct caacgacaat ttaaaagtgg tcgaaaaagc
    21361 cgataacgcg gcgcaagtca aagacgcgtt aacgaagatg cgcgccgcag cgctggatgc
    21421 gcaaaaagca acgccgccga agctcgaaga taaatcaccg gacagcccgg aaatgaaaga
    21481 tttccgccac ggtttcgaca ttctggtcgg tcagattgac gacgcgctga agctggcaaa
    21541 tgaaggtaaa gtaaaagaag cgcaggctgc tgcagagcaa ctgaaaacga cccgcaattc
    21601 atatcacaag aagtatcgtt aattcctcat ttccctgttg cctgcactca ggtaacaggg
    21661 aacttatcat cgcaaatgat gcaccacctg attgctgctg ccgcgccaga tcagggaagg
    21721 gtcttttaaa tcctgcacaa atttgccgtc gaccagcacg ttaatcaaat caacaacctg
    21781 catttgcgca gcgttgagtt cgtcgagttt atagcctgtc cacacccaga tgtctttacc
    21841 cggacactcg gcgcggatgc gttgtaccag tttcagaata tccggcacgt tttgcggatg
    21901 cagcggatcg ccgccggaga gggaaatacc ctggcgtttg atacgagtgt cattcagatc
    21961 gttaatgatc tggtcttcca ttgctttagt aaatggctga ccggaattta cccgccatgt
    22021 gcttttgtta tagcaaccgg ggcattcatg aacacacccg gagacaaaca gggtgcagcg
    22081 agtgccggga ccgttgacga tgtcgacagg atagtactga tgataattca ttatttcgcc
    22141 tggcttatca gtccgcgtgt aggctgtatc aggcaatcaa cgcctgatgc gacgctgtcg
    22201 cgtcttatca ggcctacagc cgttgccaaa tgtaggccgg ataaggcgtt tacgccgcat
    22261 ccggcaatca acgcgggatg cgacgctttc acatcacatc cggcatttgc ttagtgcaga
    22321 tcaacctatc tgcccatttc ccaaatgttt aacgcggcgc ttaacttctt cctgcttgcc
    22381 ggcgttaaac ggacgtgcat ccgggctacc taaatatccg cacacgcggc gagttaccga
    22441 cacacgggag gcgtcatggt taccacattt cgggcaggtg aagcctttgc tggtgcactc
    22501 gaactcaccg gtaaagccac actcgtagca ctcatcaatc ggtgtattgg tgccgtaata
    22561 cggtacatgc tgatagctgt aatcccagac atcttccagc gccttcaggt tgtgctgaat
    22621 gtttggatac tcgccgtagc aaatgaaacc accgttcgcc agcggcgggt aaggcgcttc
    22681 aaagtcgatc ttgtcgtacg ggttcacctt cttctccaca tcgaggtgga aactgttggt
    22741 gtagtaacct ttatcggtca cgcccggcac cacgccaaac tcagcagtat cgagacggca
    22801 gaagcggtcg cacaggtttt cactcggcgt gctgtagaga ctgaaaccat aacccgtttc
    22861 ttctttccac tgatccactg cctgacgcag acgttcaaca atggcgatac ctttcgcgcg
    22921 gagctgctcg ttgtcgtaaa catgttcgcc gccgaacagc gcgttaatgg tttcgtggat
    22981 gccgatgtaa cccagcgaaa tagacgcacg accgtttttg aagatttcag aaatatcatc
    23041 gtcagcattg agacgcacgc cacaagcacc ttccatatag aggatcgggg ccacgcgcgc
    23101 tttcacgcct tcgagacgag cgatacgggt catcagagcc ttacgtgcca gcaccagacg
    23161 ttcatccagc agcttccaga aggtggcttc atcgcctttt gcttccagag caatacgcgg
    23221 caggttcagg ctgatcacgc cgaggttgtt acgaccatcg tggatctgct cgccgttttc
    23281 attttcccac acgccgagga agctgcggca gcccatcggg gttttaaacg aaccggtgac
    23341 tttcactacc tgatcgtagt tcaggatatc cggatacatg cgcttgcttg cgcactccag
    23401 cgccagctgt ttgatgtcgt agttcggatc gcctttttta tggttcagac catcgcgaat
    23461 cgcaaacacc agtttcggga acaccgcagt tttacggttt ttaccgaggc ctgcgatacg
    23521 gttacgcagg atagattcct gaatcaggcg cgactcccag ctggtgccca gaccaaaacc
    23581 aaaggttaca aacggcgtct gaccgttggc ggtgtgcagg gtgtttactt cgtactccag
    23641 cgactggaag gcatcgtagc actctttaat ggtacgagag ttagcgtagc cttcggcgtc
    23701 cgggatgttc cactcttctg cggtcttgcg atgtttgttg tagctggcgg tgacaaacgg
    23761 tgccagcact tcatcgatac ggttaatggt ggtgccgcca taaatatggc tggcaacctg
    23821 agcaataatc tgcgcagtta ccgcggttgc cgtagagatc gacttcggcg gttcaatctc
    23881 ggcgttcccc atcttaaagc cctgggtcag catgcctttc aggtcgatca acatgcagtt
    23941 gaacatcggg aaaaacggtg agtaatcgag atcgtgatag tgaatatcgc cacgctcatg
    24001 tgcctgcacc acgtcacgcg gcagcaggtg ctgacgtgca tagtgtttag ccacgatccc
    24061 ggccagcagg tcgcgctggg ttggaatcac cttgctgtct ttgttggcgt tttcgttgag
    24121 taacgaggcg ttggtctgct cgaccagacc acggatctcc tgattcaggc gaccgcgttt
    24181 ttcacgttca atgtcgcgat cgtgacggta ctcgatgtaa gcacgagcca gttgtttgta
    24241 tggacctgac atcagctggt tttcaacagc ggtctggatc tcattgatat ccacctggtt
    24301 gcggccctgc atctgctcgc tgacaactgc agcaacagtg gcgcaataat cggcatcatc
    24361 gacttccgct gctttagctg cacgcagaat cgcttctttg atgcgctctg atttaaacgg
    24421 cactttgcag ccgtctcgtt tcatcacatg cggtgtcatg atcgctccat atttttaaga
    24481 acaggttatc cacagaaatt gggaaaggcg ttttccggtt ttttcgtttc gttttccggt
    24541 gctttccgca atcgccatcc gcttatccac aacgccgcac ctttttcgtg gggagctgta
    24601 gtagcaatta tagtcgattg atacaacata ttgggttggg acgcatttta aagtctatat
    24661 atagtgcttt gcatcaagga tgtttgaact ttttttgatg tagctcaaag taaaaagcag
    24721 agcgtacgga tgacgggtgc tacagcgtta tgtaaatttt ttaatgaatt tgctggttga
    24781 aaaatcaaca aaaacaacat gctgacagac aaaaccccgg aatgaccggc attaccgggg
    24841 cttagggaag atttacttct gtaaccacca gacagcctca aaaggccgta aagtcatggc
    24901 acagggttgt ggtgaggctt cttcgtagtt atgcatcaca agctgccagt tgccgcgcat
    24961 ttgccctagc tgccagggtt ggatctcacg gctaaggttg gcaatgacca gcaaggtttg
    25021 ccccttccat tcacggcgat agcaccacaa tacagggctg tttggcagca gatcctggta
    25081 attgccccat gtcaggatgg cttcctgctt acgcagtgcg attaactttt ggtaggtgta
    25141 aaacaccgag gactcatcgg ccagcgcggc ttctacgttg atttgttgat agttatcgcc
    25201 caggccaatc cacggttcgc cagccgtaaa cccggcatta tcgccgttgc tccattgcat
    25261 gggcgtgcgg ctgttgtcac gggatttact ggcgaggatt gccaataact cgtcggcatc
    25321 acgcccatcg ttgcgcagct cggcaaacat attgaggctc tctacgtcgc gatagtcagt
    25381 aatgcgcgtg aaatgcgggt tggtcatgcc aatctcttcg ccctggtaga tatacggcgt
    25441 tccctgcatg ccatgcagca ccatcgccag catttttgcc gcaggcacgc ggtattcacc
    25501 ttcatcacca aagcgagaaa caatgcgcgg ctgatcgtgg ttacaccaga acaaggcatt
    25561 caatgctacg ttgtgcattc cttgttgcca gtggcggaac aatgttttca acgccacaaa
    25621 gtcaggttta gccagcgtcc atttttcacc accgggataa tcgaccttca ggtgatgaaa
    25681 attaaaggtc atcgacaatt cactgcctgt cagtgccgcg tatcgctggc aatgctcaag
    25741 gctggtggag gacatttcac ctacggtcat taacccgcgt ggcgtaaaca catcgcggtt
    25801 catctcgtgc aaaaactcgt gtgctcgtgg cccgtcggtg tagaagcgac gcccgtcgcc
    25861 atccaggtca tcagggaaac gcgggtcttt ggagatcaga ttcaccacat ccaggcgcaa
    25921 cccgtcgacc ccacgatcgg cccagaactc acagactttt ttcagctctg cgcgtaccgc
    25981 tggattctcc cagttgagat ccgcctgttc tggtgcaaag agatgcaaat agtactgttc
    26041 gctttccgca tgccagcgcc acgcactacc gccaaatttt gaacgccagt tgttcggtgg
    26101 cgtttctggt tcgccatcgc gccagatata aaactggcgg taagggcttt ctttgttcag
    26161 cgcctcgcga aaccaggcat gttgggtaga ggtatggtta aacaccatat cgagaatgat
    26221 acgaatcccg cgcgattttg cctgcgtcac cagttcgtca aaatcgtcca gcgtgccgta
    26281 ggtgggatca atcgccgtat agttcgctac atcgtaaccg ttatcgacct gcggagagac
    26341 ataaaagggg gttagccaga tggcatcaac gcccagttta tgcagatagt ccaggcgttg
    26401 gataacgcca cgtaaatcgc cggtgccgct acccgtggtg tcctgaaaac tctttggata
    26461 aatctggtag ataacgccgt tttgccacca gtggggaaga ttagtcatta cgttattcct
    26521 gcaaatgcga gggggcgcaa ttgcgccccg aagaaaatta aacaatgtcc agcgtgccca
    26581 ggcggtattt ccgctgatag ataaacgagg tgagtacaat cgggatgatg atggcgatag
    26641 ccattgccag cgcaaacacc tgccagtagc tcggttgaat cgagagaatt cccggcaggc
    26701 cgcctacgcc gatgccattc gccataacgc cgttcaggcc gcatagcaat cctgccagac
    26761 cagaaccaat catcgcgcac agcatcggga agcgatattt caggttgatg ccgtacattg
    26821 caggctcagt gaccccaagc caggcggaga tcgccgcagg cacggagatc tcgcgttcat
    26881 tgtgcttgcg gctggaaatg ataatgccta tcacggcgga gccctgagcg atattcgaca
    26941 gcgcaatcag cggccacact ggcgtaccac ccatgctttg aatcatctgc aaatcaatag
    27001 caagcgtggt ctggtgtaca ccggtgatca ccagcggggc gtacaggaag ccaaacaatg
    27061 ccgcgccaat cggagcaaag ctgccggtca tcaggtgacg taccgcaaag gcaacgccat
    27121 cgccaatcat gcgaccaaac ggaccaatca gcgcatgggc gaggaacacc gcgaggatca
    27181 gcgaacagac gggcaccacc accagataga ggtaatccgg cacgatgcgt ttaaggcgag
    27241 tttcaataac gcccagcgcc agcccggcta acagtgccgg gatcacctgc gcctgatagc
    27301 ccactttggc gatgctgaac atgccaaagt cccacacttc cggcagctgc tgcccgagca
    27361 gataagcgtt catcagttgg ggggaaacca gtgtcacgcc aagcacgata ccaaggatcg
    27421 gcgtgccgcc catttttttc accgctgacc agcaaatccc gaccggcagg tagaagaaga
    27481 tcgcttcacc gatcaaccac agaaaatcgt agatcgtttg cagggaaggg tacatttgcg
    27541 ccagcgtttg accgttgctc atgggcaaat cgccgatcac attgcgaaaa ccgaggatca
    27601 aaccgccgct aatcaacgcg ggcagcaacg ggaagaagat ctctgcgaaa tgagagatca
    27661 actgctcatg ccatttcata ttctgccggg cggctttttt tacctgctct ttatcaacct
    27721 gcgcctgtcc ggttgacgct atcagtgctt gatagtaatc acccacgttg gtgccaatca
    27781 ccacctgaaa ttgcccggca ttggtgaaac agcctttcac catggggagt tgctcaattt
    27841 ctttcggtct ggcattggcc ggttggttga ggacaaagcg caggcgagta atacagtggc
    27901 tcaccgtcgc aatattgccg cgcccgccga ccagttcaat caaccgatcg atatccgttt
    27961 ggtttatttt gctcatcata aagccccatg gcagatgaca tttttggttg gctgcagaat
    28021 atattgcgcg gaaaaattta aaaacgggaa cgttcccgaa acgcagcgaa gatcacaatt
    28081 tatcgttcag gaaacgatca ggacaggttg gcggggatga tgatttgttg cggttcgctg
    28141 cgtccggtta cctgcgcgat caactggcaa gccgcctggc gtccagcttc ggcgtaacca
    28201 ggatctacgg tgacgatctc cggatgaagg aatttcatta acggcgtatt accgacgctc
    28261 gccagttgca aggtgtcgat gcgttgctct tgcaggtatt tacttgcgcc aagtgccagc
    28321 gtgtcggttg cgcacagtaa ggcggtagtt tcaggcgtaa tcacttttgc tacgttctca
    28381 tagccttgct tcatagcaag ccctggcagg gcggcaacgg gatgcagttt atgcgctttg
    28441 cagaacgcca ggtaggcttc gtgacgtcgc ttaccggttg tcacgtcact gtgcggcacg
    28501 ccgagataac tgatattacg atgcccctgg tcatacagcc gttgcatcag gatcttgatt
    28561 gccccttcgt cgtcataaca gaccgaagca aagccttttg cgtcacgcgc cagcagaacc
    28621 agcgatgact gccagtgggc taacatttct tctgttatgc cagtaaaacc gaacagcact
    28681 acgccgtcga tattacgccg tttcagcacc cccaaatgtt cggcaactaa ttgcggggaa
    28741 aactgacttt ccatcatgat tgggtcgtaa ccttgttcat agaacgctgg cagcatggtt
    28801 tgaacggcga gattttctga caacgaatcc agacgggtaa caatgatggc gaccacttta
    28861 tcgctttgcc cacgcatagc gcgcgcagaa cgggaagggg aaaatccatg ctgattcatc
    28921 actgcttcaa cacgctcgcg ggtgcgctgg ctcacgccgc tttcgttatt cagcacccgg
    28981 gaaactgtag atttccccac gccgcttaag cgcgcgatgt ctttgatggt cagccgattt
    29041 tgcatcctgt tgtcctgtaa cgtgttgttt aattatttga gcctaacttt acccgtgcat
    29101 tcagcaatgg gtaaagtctg gtttatcgtt ggtttagttg ccagcaggta ttatatcgcc
    29161 atagatgcta cgaatattat tggattctcc ttattatttg cggcgctttt ttcacttacc
    29221 ggaggttata tggaacctga tcccacgcct ctccctcgac ggagattaaa acttttccgg
    29281 taagcccgtc ttttcacggc gttaccggat gcgtaaggcc gtgacgtttt aacgtccctg
    29341 ctcagcttta ttaccttcag gtaaggcttc gccacgcctg aagacatttc tgtactgttt
    29401 cagacagtgc ggagggactc cttatgttta aagaaatttt tacccggctc attcgccatt
    29461 taccttcccg tctggttcat cgtgatccat tgcctggcgc gcagcagaca gtgaatacgg
    29521 tggtcccgcc gtccttaagt gcgcattgcc tgaaaatggc ggtgatgccc gaagaagaat
    29581 tgtggaaaac gttcgacacc catccggaag ggttaaatca ggcggaagtg gaatctgccc
    29641 gcgaacaaca tggtgaaaat aaattacccg cacaacaacc gtcgccgtgg tgggtacatt
    29701 tatgggtctg ctatcgcaac ccctttaata ttttactcac cattctcggc gctatttctt
    29761 acgccacgga agatttattt gccgcgggcg ttatcgcgct aatggtcgct atttcaacgt
    29821 tgctgaactt tattcaggaa gcacgttcca ctaaagcggc agatgccctg aaagcgatgg
    29881 tcagcaatac cgcgacggtg ctgcgcgtaa ttaacgacaa aggcgaaaat ggctggctgg
    29941 agatcccgat cgaccagctg gtgcccggcg atattataaa actggcggcg ggagatatga
    30001 tcccggcaga tttacgtatc ttgcaggcgc gggatctgtt cgtcgctcag gcgtcgttaa
    30061 ccggtgagtc tctgcccgtg gaaaaagccg ctaccactcg ccagccggag cacagcaatc
    30121 cgctggagtg cgacacgctg tgttttatgg gcaccaccgt ggtgagcggc acggcacaag
    30181 caatggtgat tgctacaggt gccaatacct ggtttggtca actggcgggg cgtgttagtg
    30241 agcaggaaag cgagccgaat gcctttcagc aagggatcag ccgcgtcagt atgctgctga
    30301 ttcgctttat gctggtgatg gcaccggtgg tgctgttaat caatggctac accaaaggcg
    30361 actggtggga agcggcgctg tttgcgcttt cggtcgcggt aggcctaacg ccggaaatgt
    30421 tgccgatgat tgttacctcg acgctggcgc gcggcgcagt aaagctgtcg aaacagaaag
    30481 tgatcgtcaa acatctggat gctattcaga actttggcgc aatggatatt ctgtgcactg
    30541 ataaaaccgg caccctgaca caggataaaa ttgtgctgga gaatcatacc gatatctccg
    30601 gtaaaaccag cgaacgcgtg ctgcatagcg cgtggttgaa tagtcattat cagaccggac
    30661 ttaaaaacct gctcgataca gcggtgctcg aaggtacgga tgaagagtca gcgcgctcgc
    30721 tggccagtcg ttggcaaaaa atcgatgaga ttccgtttga tttcgagcgt cgccggatgt
    30781 cggtggtagt ggcagaaaat accgagcacc atcagctggt ttgcaaaggt gcattgcagg
    30841 aaatcctcaa tgtgtgttcg caggtgcgtc acaatggcga gattgtgccg ctcgatgaca
    30901 tcatgctgcg taagattaag cgggttactg atacgctgaa tcgtcagggg ctgcgcgtgg
    30961 ttgcggtggc gacgaaatac ctgccagcgc gtgaaggaga ttaccagcgg gcggatgaat
    31021 ccgacctgat cctcgaagga tatattgctt tccttgatcc gccaaaagag acaaccgctc
    31081 cggcactgaa ggcattaaaa gcgagtggaa ttaccgtaaa aatccttact ggcgacagtg
    31141 agttagttgc agcgaaagtg tgccatgaag tggggctgga cgcgggagag gtggtcattg
    31201 gtagtgatat tgaaacgcta tctgacgacg aactggcaaa tcttgcacag cgcactacgc
    31261 tgtttgcccg cctgacgccg atgcataaag aacgcattgt gaccttactg aagcgcgaag
    31321 ggcatgtggt tggctttatg ggcgatggta ttaatgatgc gcccgcctta cgcgctgcgg
    31381 atatcggcat ttctgtggac ggcgcggtag atattgcccg tgaagcggct gatatcatcc
    31441 tgctggaaaa aagcctgatg gtgctggaag agggggttat tgagggacgt cgcactttcg
    31501 ccaacatgct gaaatacatc aaaatgacgg cgagttctaa cttcggtaat gtgttcagcg
    31561 tgctggtggc gagtgctttc ttgcccttcc tgccgatgtt gccgttacac ttgcttattc
    31621 agaacctgct gtacgatgtg tcacaggtgg cgatcccgtt tgataacgtc gacgacgagc
    31681 aaattcaaaa gccgcagcgt tggaatccgg cggatctggg gcgctttatg atcttcttcg
    31741 gaccgatcag ctcgatcttc gatattctga cgttttgcct gatgtggtgg gtattccatg
    31801 ccaacacgcc ggaaacgcaa acgctgttcc agtcgggatg gtttgtggtg ggcttactgt
    31861 cgcaaacgct gattgtgcat atgatccgca cccgccgtgt gccgtttatt cagagctgtg
    31921 catcgtggcc gttaatgatc atgaccgtga tcgtgatgat tgtcgggatc gcattgccgt
    31981 tttcaccgct ggccagttat ctgcaattac aggcgctgcc gttaagctat ttcccgtggc
    32041 tggttgcgat tctggcaggg tatatgacat taacccagtt ggtgaaaggg ttctatagcc
    32101 gtcgttacgg ctggcaataa agaataaact gggcacgata gccccgattc agattgcaga
    32161 caaagtaagc tttgttcatg ccggatgcgg cgtaaatgcc ttatccggcc aacaaattca
    32221 ataaattgca ggaatcacgt aggcctgata agcgtagcgc atcaggcgat tttgcgtttg
    32281 tcatcagact taatccgggc atgatagccc ggatttccat caagattagc gacgaacagc
    32341 gatcgcttcg atctcaatct tcacgtcttt cggcagacgg gcaacttcaa cgcaagaacg
    32401 tgccgggaag gtggcgttgt gttcggtgaa gaaggcttcg taagtggcgt ttacggttgc
    32461 gaagtcgttc agatctttta caaacacggt agttttaacg atgtcgccca ctttcagacc
    32521 agcggcttcg acgatcgctt ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc
    32581 tgccggtact tcgcccgttt tcggatttac cgggatctga ccggaagtga tgatcatatt
    32641 gcccagatca acgccctgta cgtaaggacc gatagctgcc ggtgcatttt ccgtcgcgat
    32701 agttttgctc atgatttctc ctttattaca gcggtaaaaa gtctggctca ttatagggag
    32761 ccagactttt attaccaacc gcaattaatt ggccagcacc acattatggg aaaactcttt
    32821 ttcacagtat ttgcatttga gcgcgatatc attggcgcgt tttcgcacgg caaagctgga
    32881 agaaaccggt tcggcatggc tgatacagtt gctgttcggg cagaccagca cattgtcgat
    32941 gcgctccggc agacttgggc gcgatttacc caccacttca tagttgtcga tacggttaac
    33001 cgtggcttgc ggcgcataca atgccagttg atctacctgg tcttcactca aaaaggtatt
    33061 ttcgattttg atcagatctt tgcggcccat ctcgccagaa ggcaggttca gaccgatggt
    33121 gatgcgctga tccgtttcgg tcagcttgaa cagactcaac agcttaaaac cgatctgggc
    33181 ggggatatgg tcaattaccg tgccgcgttt aatagcttca acctgcaatt tattatcgtg
    33241 tgtcatctct atttcccctt acagtaccag atcgcgattc agaaccagtg ccagtaacgc
    33301 ctggcgagcg aaaatcccgt tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc
    33361 aacatccgtc gcaatctcat caacacgcgg cagcggatga agtactttca tattggcttt
    33421 ggcgttgtgg agatcgctgg cgcgaagaac aaactgcgct ttcacgttgg cgtactcgga
    33481 cgggtccaga cgctcttttt gcacgcgggt catgtacagg atgtcaactt ccgccatcac
    33541 ttcttcaata gagctgtgca gactccatgc gatccctttt tcatcaagca tatccagaat
    33601 gtattgcggc attgccagcg cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt
    33661 cgctaacgcc tgagtcaggg agtgaacggt gcggccatat ttcaggtcac caaccattgc
    33721 gacgtggaga ttgtccagac gcccctgggt ttcctgaatg gtgaataagt ccagcaaggt
    33781 ttgcgtcgga tgttggttgg agccatcacc ggcattcagt accggtacat tgccggaaaa
    33841 ctcggtggcc aggcgcgccg caccttcctg cggatgacgc atcactatcg catcgacgta
    33901 agtgctgata accgaaatgg tatcggccag cgtttcgccc tttttaccca gtgatgtatt
    33961 ggcgctgtcg gagaagccca ccacgctggc acccaggcgg tgcatggaag tttcgaaaga
    34021 gaggcgggta cgggtagagg cttcgaagaa acagctggca atgactttgt gcttcaacag
    34081 ctctggttgc gggtttgctt tcagtttcgc cgctgtcgcc agcaccagat taaggtcatc
    34141 gcgactaagg tcgtttatgg aaatgatatg tttctgatat agcggattag ccatctttta
    34201 tctcctgacg cctgggcaaa aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa
    34261 cgggaagaaa aacggcaggc cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac
    34321 acactgaacc atacatcctc ccggcaaatt gtccggcatt atactcatcg tcagaagcgg
    34381 tgcaagcatt tgatgcaata ttttgtcagc gcaaacggtt tatttgaatt ataagtcaaa
    34441 atatatgcat ttttatttac tgtgattctg gaggggaact gtcgttcggt atcaataatt
    34501 gcagacattt cctgcttttt tctttttcac tttcacgcaa tcagatatgc attttattcc
    34561 attcatccga cttatagggc ggagtgtgaa aacgaacggc taacactatt gcttacagct
    34621 cagggatgcg cgctatcact ttaatttcaa aatcaaagcc tgccagccat gtaacgccca
    34681 ccgccgtcca ggttggataa ggtggcgcgc taaatatttc atttttcacc gtcatgatgt
    34741 cttcaaattg tttttctgga tcggtatgaa agctcgtaac atcaatgata tcgtcaaaag
    34801 tgcatcccgc agctgccagg gtcgcatgca aattatcaaa tgccagtctg acttgttgct
    34861 gaaaatcggg ttctggtgtt ccgtcctctc gacttcctac ttgcccggaa acaaacagca
    34921 aatcgccgga acgaatagcc gcagaataac gatgttctgc atacagtgaa tgtcggccag
    34981 cagggaaaac agcggttctt tctaccattt ggttatcctc aagatttacg acatgaacag
    35041 aagatttata tttaccagga gccgctttta gcggacgacg tgagtaaaca aaacccagac
    35101 atcatgggaa atggctgggc ttaattgagc gtagtcggtt atgcgccaaa cgcgccatca
    35161 atggtatgca tcgcgccggt aacaaaactg gcttctggcc ctgctaacca tgcgaccata
    35221 ccagcgacct cttccggttg cccatgtctt ttgatagcca taaaactatg caacatatcg
    35281 cgcattggcc cgttggcggg attagcgtcg gtatcaattg gccctggctg gacgacgttg
    35341 atggtgatcc cacgcggtcc aaaatcacgg gccagcccgc gcgccatgcc ttgtagggca
    35401 gatttactgg cggcataagc agccatgcct gcaacaggca tacgatcgcc attcacggag
    35461 ccgatgatta agatgcgccc accttcgggc atctgccggg cggcttcaac agaggcatgg
    35521 taaggagcat gaatattgat tttgaaaagg cgatcaatat cgtcggcatt taattccagg
    35581 gcatcgccaa agacgccaat acctgcattt accaccagga tatccaatgc gccgctctta
    35641 cgaacgacat caatgacagc gtctctgtca gcactatctg taaatactgc tgtcgctccc
    35701 gtctcttgtg ccaggcgttc agcggcatct ttcgaccccg cataggtgaa tcgtacattg
    35761 gccccatcgg tgacgaaacg acgtacgata gcggcaccga ttccacgact gccaccgagg
    35821 atgagaactg tcttacctgt aaaagcgccc ataaggactc cttgatttat tatgtaacat
    35881 gcattacaaa actgttttaa ctttctgtca acaagttttg taatggttac taaaaaacaa
    35941 tctcgcgttc caggtcgtcc cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa
    36001 gtgctttttc accaaaaagg tttcgatgct gtcagtgttg ctgaagttac tgattatctt
    36061 ggtattaacc ccccgagcct ctacgcggct tttggcagta aggctgggtt atttagccgt
    36121 gtactcaatg aatatgtcgg tacggaagct attccgcttg tcgatattct tcgtgatgat
    36181 cgtccggtag gggagtgcct ggctgaggta ttaaaagaag cggcgcgcag atatagccaa
    36241 aacggcggct gcgctggctg tatggttctt gaaggcattc atagtcatga tccacaagcg
    36301 cgtgatattg ccgttcaata ttatcacgcc gcagaaacgg ccatttatga ctatatcgcc
    36361 aggctgcatc cacaaagcgc acaatgtgtg actgatttta tgagtaccgt gatgtcaggg
    36421 ctttctgcga aggcacgaga ggggcactcc ctcgaacaac tctgtgcaac tgctgcactg
    36481 gcgggggaag cgataaaaac tattctcaag gagtgatgct ggccttgatc cgaaaggcgg
    36541 gaacgcgcct gccgataagt tgtgataaga caataattca cgcgttaagg ctggctgaat
    36601 tgatcatctt ttcgtataac ggtggaaatg aaacattgtt ttaattaagg agtggaaaag
    36661 atgatcatcg gaaatattca taatcttcag ccgtggctac cccaggagtt acgccaggcg
    36721 attgagcata tcaaagcaca cgttacggca gaaacgccaa agggcaagca cgatatcgaa
    36781 ggtaatcgtc tgttttatct tatctcggaa gatatgaccg agccgtacga agctcgccgt
    36841 gcggagtacc atgcccgcta tctcgacatt cagattgtgt taaaaggtca ggaaggcatg
    36901 accttcagca cacaaccggc aggtacgccg gataccgact ggttagctga taaagacatc
    36961 gcatttttgc cggaaggcgt tgatgagaaa acagttatcc ttaatgaagg tgattttgtt
    37021 gtgttttatc cgggggaagt gcataaaccg ctgtgcgcag tgggtgcacc agcccaggtt
    37081 cgcaaagcag tagtgaagat gctgatggcg tgatgacttt tcgccgtaaa taactccagg
    37141 tttacggcga gtttgtgaaa agagcgtttt ttgatatttt tttgtgagta aaatttgtaa
    37201 tgcttagacg ttcttattaa ctcaaggagt tcgtcat
//
LOCUS       DBFIFS010000025        37137 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.025, whole genome shotgun sequence.
ACCESSION   DBFIFS010000025 DBFIFS010000000
VERSION     DBFIFS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.025"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(234..479)
                     /gene="ymjA"
                     /locus_tag="N7A07_001136"
     CDS             complement(234..479)
                     /gene="ymjA"
                     /locus_tag="N7A07_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGU2475840.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(613..1998)
                     /gene="puuP"
                     /locus_tag="N7A07_001137"
     CDS             complement(613..1998)
                     /gene="puuP"
                     /locus_tag="N7A07_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU2475841.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(1988..2152)
                     /locus_tag="N7A07_001138"
     CDS             complement(1988..2152)
                     /locus_tag="N7A07_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475842.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(2301..3719)
                     /gene="puuA"
                     /locus_tag="N7A07_001139"
     CDS             complement(2301..3719)
                     /gene="puuA"
                     /locus_tag="N7A07_001139"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU2475843.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            3931..4695
                     /gene="puuD"
                     /locus_tag="N7A07_001140"
     CDS             3931..4695
                     /gene="puuD"
                     /locus_tag="N7A07_001140"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU2475844.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            4722..5279
                     /gene="puuR"
                     /locus_tag="N7A07_001141"
     CDS             4722..5279
                     /gene="puuR"
                     /locus_tag="N7A07_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU2475845.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            5429..6916
                     /gene="puuC"
                     /locus_tag="N7A07_001142"
     CDS             5429..6916
                     /gene="puuC"
                     /locus_tag="N7A07_001142"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU2475846.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            6918..8198
                     /gene="puuB"
                     /locus_tag="N7A07_001143"
     CDS             6918..8198
                     /gene="puuB"
                     /locus_tag="N7A07_001143"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU2475847.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            8236..9501
                     /gene="puuE"
                     /locus_tag="N7A07_001144"
     CDS             8236..9501
                     /gene="puuE"
                     /locus_tag="N7A07_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU2475848.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(9632..10609)
                     /gene="pspF"
                     /locus_tag="N7A07_001145"
     CDS             complement(9632..10609)
                     /gene="pspF"
                     /locus_tag="N7A07_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU2475849.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            10776..11444
                     /gene="pspA"
                     /locus_tag="N7A07_001146"
     CDS             10776..11444
                     /gene="pspA"
                     /locus_tag="N7A07_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU2475850.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            11498..11722
                     /gene="pspB"
                     /locus_tag="N7A07_001147"
     CDS             11498..11722
                     /gene="pspB"
                     /locus_tag="N7A07_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU2475851.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            11722..12081
                     /gene="pspC"
                     /locus_tag="N7A07_001148"
     CDS             11722..12081
                     /gene="pspC"
                     /locus_tag="N7A07_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU2475852.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            12090..12311
                     /gene="pspD"
                     /locus_tag="N7A07_001149"
     CDS             12090..12311
                     /gene="pspD"
                     /locus_tag="N7A07_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU2475853.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            12386..12700
                     /gene="pspE"
                     /locus_tag="N7A07_001150"
     CDS             12386..12700
                     /gene="pspE"
                     /locus_tag="N7A07_001150"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU2475854.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            12910..14588
                     /gene="ycjM"
                     /locus_tag="N7A07_001151"
                     /pseudo
     CDS             12910..14588
                     /gene="ycjM"
                     /locus_tag="N7A07_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            14602..15894
                     /gene="ycjN"
                     /locus_tag="N7A07_001152"
                     /pseudo
     CDS             14602..15894
                     /gene="ycjN"
                     /locus_tag="N7A07_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            15915..16796
                     /gene="ycjO"
                     /locus_tag="N7A07_001153"
     CDS             15915..16796
                     /gene="ycjO"
                     /locus_tag="N7A07_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU2475855.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            16783..17625
                     /gene="ycjP"
                     /locus_tag="N7A07_001154"
     CDS             16783..17625
                     /gene="ycjP"
                     /locus_tag="N7A07_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU2475856.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            17656..18708
                     /gene="ycjQ"
                     /locus_tag="N7A07_001155"
     CDS             17656..18708
                     /gene="ycjQ"
                     /locus_tag="N7A07_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU2475857.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            18726..19514
                     /gene="ycjR"
                     /locus_tag="N7A07_001156"
     CDS             18726..19514
                     /gene="ycjR"
                     /locus_tag="N7A07_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU2475858.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            19524..20579
                     /gene="ycjS"
                     /locus_tag="N7A07_001157"
     CDS             19524..20579
                     /gene="ycjS"
                     /locus_tag="N7A07_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2475859.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            20576..22843
                     /gene="ycjT"
                     /locus_tag="N7A07_001158"
     CDS             20576..22843
                     /gene="ycjT"
                     /locus_tag="N7A07_001158"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU2475860.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            22840..23499
                     /gene="pgmB"
                     /locus_tag="N7A07_001159"
     CDS             22840..23499
                     /gene="pgmB"
                     /locus_tag="N7A07_001159"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU2475861.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            23513..24595
                     /locus_tag="N7A07_001160"
     CDS             23513..24595
                     /locus_tag="N7A07_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2475862.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            24640..25545
                     /gene="ompG"
                     /locus_tag="N7A07_001161"
     CDS             24640..25545
                     /gene="ompG"
                     /locus_tag="N7A07_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU2475863.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(25656..26654)
                     /gene="ycjW"
                     /locus_tag="N7A07_001162"
     CDS             complement(25656..26654)
                     /gene="ycjW"
                     /locus_tag="N7A07_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2475864.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            26809..28206
                     /gene="ycjX"
                     /locus_tag="N7A07_001163"
     CDS             26809..28206
                     /gene="ycjX"
                     /locus_tag="N7A07_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU2475865.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            28203..29264
                     /gene="ycjF"
                     /locus_tag="N7A07_001164"
     CDS             28203..29264
                     /gene="ycjF"
                     /locus_tag="N7A07_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU2475866.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            29412..30953
                     /gene="tyrR"
                     /locus_tag="N7A07_001165"
     CDS             29412..30953
                     /gene="tyrR"
                     /locus_tag="N7A07_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU2475867.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(30997..31503)
                     /gene="tpx"
                     /locus_tag="N7A07_001166"
     CDS             complement(30997..31503)
                     /gene="tpx"
                     /locus_tag="N7A07_001166"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU2475868.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            31622..32587
                     /gene="ycjG"
                     /locus_tag="N7A07_001167"
     CDS             31622..32587
                     /gene="ycjG"
                     /locus_tag="N7A07_001167"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU2475869.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(32562..33290)
                     /gene="mpaA"
                     /locus_tag="N7A07_001168"
     CDS             complement(32562..33290)
                     /gene="mpaA"
                     /locus_tag="N7A07_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU2475870.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(33581..34219)
                     /locus_tag="N7A07_001169"
     CDS             complement(33581..34219)
                     /locus_tag="N7A07_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2475871.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(34240..35170)
                     /locus_tag="N7A07_001170"
                     /pseudo
     CDS             complement(34240..35170)
                     /locus_tag="N7A07_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(35170..36090)
                     /locus_tag="N7A07_001171"
     CDS             complement(35170..36090)
                     /locus_tag="N7A07_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2475872.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            36228..37127
                     /gene="pgrR"
                     /locus_tag="N7A07_001172"
     CDS             36228..37127
                     /gene="pgrR"
                     /locus_tag="N7A07_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU2475873.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT         9073 a         9224 c         9933 g         8907 t
ORIGIN      
        1 cgtaatgagg gccgtgtggt tggtcgttga acagatggcg tcgccatctg ttcaacattc
       61 gtacccgtga tttctctata actataactc acagaacaac ttagcgagga gcagagcggt
      121 acgatcgtga gaaacccatt aaagcacgct gcagtgcgtg tcattgttag ccggatgcgg
      181 cgtgaacgct ttatccggac aacgatactg accgatcgtc tgcaattaaa taattactca
      241 ttaccccatt gattcaaaaa ctctgcgatc tcatcaatgc gtacgggatt aatccccgct
      301 tcagcagcca tttcatgctg ggcttcttcg ctgatctctt cattgttcat caaacgggtg
      361 agcagtaact ggaagtagtg ggccagcggt gtgcgttcag cgtctgcgac aggctctgcg
      421 cattcggtcg cgtactcatc ggcaatatca aaatatttta gcgggatatc gtggttcatt
      481 atttgcccct ggggttaatg cgctaagcat aagggcggat gatagcactc gtgatccggg
      541 tcatacatct ttttatagtt aatggcgcgg cgcattaccc tcaggcagga taatgcggcg
      601 cgcatccgac tattacgttt cgctcactgg cgttctgtca ccttcgtaca gcggcacttt
      661 gcgatagcgg cggatcaaat accacaaata tgcgccgcct aacgaagccc ataccagacc
      721 gagtgtcagt gacgttgact cgaggttaac ccacagcaca cccaccgtca gcgcgccaac
      781 cagcggcatc agcaaatagt ggaagtgatc cttccagctt ttattcattc ctttacgccg
      841 ccagaaatga ttaaacaccg acagattcac gaaggtaaac gccaccagag caccgaagtt
      901 aatcaatgct gtcgcggtga ctaaatcaaa gaacaatgcc gacatcgcga caattccgac
      961 cataatgacg ttcagtgccg gagtccgcca ttttgggtgc acatagccaa acacgcgctc
     1021 cggaaacaca ttgtcgcgcc ccatcacata cagcagacgt gacacgctgg catgtgaggc
     1081 caggccagac gctaacgtgt tcacaaacgt ggtgcagagg aaaatcgact ggaacagctt
     1141 accgccgacg tagagtgcaa tttcaggcag tgcggcgtcc gggtctttaa agcggctgat
     1201 atcggggaag aacagctgca taaagaacga cgccgcgata aagataacgc caccatagac
     1261 cgccgtcagg aagatggctt tcgggatcac gcgtgcggcg tctggtgtct cttccgaaag
     1321 tgtggtcacc gcatcgaaac cgaggaacga gaaacagaca atcgtcgccc cggtaataat
     1381 cgggatcagg tgcgcgttct cgctgataaa cggctgaagt gaccagacgg tgccaacgcc
     1441 ttctccttta tgcagtccct gaaccaccag gaagataaac accaccatga tggagatttg
     1501 caccagtaca aacagggtat tgaagttagc gaccaggttg acgctcttca gattcgcggc
     1561 ggttaaaatg gcgacgaagg ttaccaccca cacccacggc ggcacttccg ggaagagggc
     1621 ggagagatag attttcgcca acaaaacgtt gatcatcggc aaaaagagat aatccagcag
     1681 tgatgaccag ccgaccataa atccgacgtg cgggttaatc gacttttgcg cgtaggtata
     1741 ggccgaaccg gcctccggaa actggcgaac cagtttgccg tagctgatag cggtaaacag
     1801 cacgcccgcc agcgccagca aataggacgc agggacgtga ccgtcgctaa tgccagatac
     1861 aatgccaaag gtatcgaaca cagtcatcgg cgtgagatag gccagaccca tcatcacaac
     1921 ctgccacaat ttcagtgatt ttcgcagacg ggttttgccg ggttgcgcag caatattcag
     1981 tggtgaatta atagccataa ttgctgcctc ccctgcgtaa taaggtttgc ggtcgtgtcg
     2041 acctgaaatc aaacctgttt agcaagggaa gcaaaggggg cggagtccag tcagtcgcag
     2101 gaggggcgca tacgcagcct cccgcgccat gcggggattt aatcatcggc atcatctcat
     2161 ttcctcgtca cagtcttaat cgttaacgga agcccgttgc tgcctgcgct ccataacgcg
     2221 acatccggga aatgtctgga ttaccagcaa atgtcaggcc tggctccgct caggccgatg
     2281 aaacaacccc gcaaggggta ctatgcgttt ttcaacatcc actcaatttc tgtttctgtg
     2341 atgaggcgct caaactgcaa cagctcatca tttttacagg cgtgatacac atggcaaaac
     2401 cgttcgccta aatagcggcg caggtgatca ttctcgataa actcacccag ggcatcgctc
     2461 tggcgaatcg ggaagggtaa gccttcctgt tccagcccgt taccttcgac ttcttcctgc
     2521 aacggcagct cgttatcaag gccatgcaaa ataccggcaa aaatcgctgc catcaccaga
     2581 tatgggttgg catcggcacc cgccacgcga tattccacgc ggtgattatg acgatcgccg
     2641 catggaatac gcagggcgac ggtacggtta ttatggcccc acgacgcctg cgtcggtaca
     2701 tacatccccg gctggaagcg gcgatacgag ttcacgtttg gtgccagcaa cgccattgat
     2761 gacggcatca ggtcaatcat cccggcgagc atctttttca gcagcggcga atcttcgcct
     2821 tctgtgtcag aaagcacgtt ctcgccacga ttattttgca tactgatatg gatatgcatt
     2881 ccgctgcccg cgtgctcttc atacggcttc gccataaaag tggcgtgcat cttatgcttt
     2941 tctgccatca gacgcaccag gcgttttagt gccagcgcat catcgcaggc ttccagcacg
     3001 ttatcagtat ggtaaaggtt gatttcaaac tgacccggcg aggcctcagc gaccgcgcca
     3061 tctgccggaa tcagctgtaa ctgcgccagt tcatcaatat cattgagcac gtcggcgaag
     3121 tggttgaggt tatcaacgga gtaaacctgg ctttgtgtat tgcggtcatc agtgccgggc
     3181 gcgcagggcg gttgcagata cccttcagcg tcgcgctggc gatccagtaa atagaactcc
     3241 agctctaccg ctacgaccgg gaacaatccg cgctggcgca gctgctgcca caggcggtta
     3301 agaacgttcc gcggctcaac gtcaaaggga gcgccatctt catcgaccat ggtcaggagc
     3361 atctgaccga taaactctgg atcggcggca gaaggggtta aggaaccgag aacaggaaca
     3421 caggtacgat ccggctcgcc catttcctga cccagacctg cttcttctac tacgttgccc
     3481 agaatatcca ttgcaaatac cgaggccggg aaataacacc ctttctcgag cttcttcagg
     3541 cttgaaacag gaatacgttt tccacggaag caaccattca aatcggtaag taaaacatca
     3601 acatattgcg tattcgggta gcgctccagg tagcgtttca cttcctgcgt aaaggcgcta
     3661 ccccgcctct cttctgactg ctgaacaaag ttctctactt caacgatatt ggtttccatg
     3721 attcttcgcc tttggtttgt tttccgctcg ttatcaaagc gtaaaatata atgaccacca
     3781 ttcgaatctg tatgcaaact aaatgtttgt caaatgttaa attgagtttg caaaaatgaa
     3841 aacccactgc tagattgaaa aaatattgaa cataaaggtc atttaaaccg cagtaatggc
     3901 gataatttag tccactttgt gagattgaga atggaaaata taatgaacaa tccggttatc
     3961 ggtgtcgtaa tgtgcaggaa caggcttaag gggcatgcga cccagactct gcaagaaaag
     4021 tacctgaatg ccatcatcca cgcaggcggc ttgccaattg cgctgccaca tgcgctggcg
     4081 gaaccgtcgt tacttgaaca acttttgccg aaactcgatg gcatttacct tcctggtagt
     4141 cccagcaatg tgcagccgca cctatatggt gaaaacggcg atgagcctga cgccgatccc
     4201 gagcgtgatc ttctgagcat ggcgttaatt aatgccgcac tcgaaaggcg catccccatt
     4261 ttcgccatct gccggggttt acaggaactg gtggtggcaa ccggtggttc gttgcatcgc
     4321 aagctgtgcg aacagcctga attgctggaa catcgggaag atcccgaact gccggtggaa
     4381 cagcaatatg caccgtcgca tgaagttcag gttgaagagg ggggattgct gtctgcattg
     4441 ttacctgaat gtagcaactt ttgggtaaac tctctgcatg gacaaggggc gaaggtcgtt
     4501 agcccacggt tgcgtgttga agctcgctcg ccggatggct tagttgaggc ggttagtgtc
     4561 atcaatcatc cttttgcgct gggcgtacag tggcacccgg aatggaacag tagcgagtac
     4621 gcgctttcgc gtatattgtt cgagggcttt atcaccgctt gtcagcacca tatcgctgaa
     4681 aaacagcgac tctgaccact acagtttaag gaaatgcaaa tatgagtgat gagggactgg
     4741 cgccaggaaa acgcttgtcg gaaatccgcc agcagcaggg gctttcacaa cgtcgtgccg
     4801 ccgaactctc cgggctgact cacagtgcta tcagtacgat agaacaagat aaagtcagcc
     4861 ctgccatcag tacgctgcaa aagctgctga aggtgtatgg tctgtcactc tcggaattct
     4921 tttccgagcc ggaaaaacct gatgagccgc aggtcgtcat taatcaggac gatttaattg
     4981 agatgggcag tcagggtgtg tcaatgaagc tggttcataa cggtaacccg aatcgcacgc
     5041 tggcgatgat ctttgaaacg taccagccgg gcacaaccac tggggaaaga attaagcatc
     5101 agggtgagga aataggcact gtactggaag gtgaaattat tctgacgatt aatggtcagg
     5161 attaccacct cgtcgcgggg caaagttatg ccattaatac cggcatcccg cacagtttca
     5221 gtaatacgtc ggcaggtatt tgccgaatta tcagcgccca tacgcccacc acgttttaat
     5281 ctttttgttc tgtaagccgg gtaagcgcaa tgcgcccggc aatccataaa gaatgtcgcc
     5341 agaaataaac gggcatacgg cccggggatc tctgcgccct gacgttcaca aactgcatat
     5401 atctgataga cgtgaaacag gagtcataat gaattttcat catctggctt actggcagga
     5461 taaagcgtta agtctcgcca ttgaaaaccg cttatttatt aacggtgaat atactgctgc
     5521 ggcggaaaat gaaacctttg aaaccgttga tccggttacc caggcaccgc tggcgaatat
     5581 tgcgcgcggc aagagcgtcg atatcgaccg tgcggtgagc gcagcacgcg gcgtatttga
     5641 acgcggcgac tggtcactct cttctccggc aaaacgtaaa gcggtgctga ataaactcgc
     5701 cgatttaata gaagccaacg ccgaagagct ggcactgctg gaaactctcg acaccggcaa
     5761 accgattcgt cacagtctgc gtgatgatat acccggcgcg gcgcgcgcca ttcgctggta
     5821 cgccgaagcg atcgacaaag tgtatggcga agtggcgacc accagtagcc atgagctggc
     5881 gatgatcgtg cgtgaaccgg tcggcgtgat tgccgccatc gtgccgtgga acttcccgct
     5941 gctgctgact tgctggaagc ttggcccggc gctggcagca gggaacagcg ttgtcctgaa
     6001 accgtctgaa aaatcaccgc tcagtgcgat tcgtctcgcg gggctggcga aagaagcagg
     6061 cttgccggat ggtgtgttga acgtggtgac gggttttggt catgaagcgg ggcaggcgct
     6121 gtcgcgtcat aacgatatcg acgccattgc ctttaccggt tcgacccgta ccgggaaaca
     6181 gctgctgaaa gatgcaggcg acagcaacat gaaacgcgtc tggctggaag cgggcggcaa
     6241 aagcgccaac atcgttttcg ctgactgccc ggatttgcaa caggcggcaa gcgccaccgc
     6301 agcaggcatc ttctacaacc aggggcaggt gtgcatcgcc ggaacgcgcc tgttgctgga
     6361 agagagcata gccgatgaat tcttagccct gttaaaacag caggcgcaaa actggcagcc
     6421 gggccatcca cttgatcccg caaccaccat gggcacctta atcgactgcg cccacgccga
     6481 ctcggtccat agctttattc aggaaggcga aagcaaaggg caactgttgt tggatggccg
     6541 taacgccggg ctggctgtcg ccatcggccc gaccatcatt gtggatgtag acccgaatgc
     6601 gtccttaagc cgcgaagaga ttttcggtcc ggtgctggtg gtcacgcgtt tcacatcaga
     6661 agaacaggcg ctacagcttg ccaacgacag ccagtacggc cttggcgcgg cggtatggac
     6721 gcgcgacctc tcccgcgcgc accgcatgag ccgacgcctg aaagccggtt ccgtcttcgt
     6781 caataactac aacgacggcg atatgaccgt gccgtttggc ggctataagc agagcggcaa
     6841 cggtcgcgac aaatccctgc atgcccttga aaaattcacc gaactgaaaa ccatctggat
     6901 aagcctggag gcctgaaatg acagaacata ccagcagtta ctacgccgcc agtgcgaata
     6961 aatacgcgcc attcgacacg ctgaatgagt caatttcctg cgacgtttgc gtggttggcg
     7021 gcggctatac cgggctgtct tccgcactgc atctggcgga agcgggcttt gacgtggtgg
     7081 ttctggaagc ctcacgcatc ggctttggcg ccagcgggcg caacggcggg cagcttgtga
     7141 actcctacag ccgcgacatc gacgttatcg aaaaaagcta cggcatggac accgcccgta
     7201 tgctcggcag catgatgttc gaaggtggtg agatcatccg cgaacgtatc aaacgttatc
     7261 agattgactg cgactaccgc cccggcggtc tgtttgtggc gatgaatgat aaacagctcg
     7321 ccacacttga agagcagaaa gagaactggg aacgctacgg caataaacag ctggagatgc
     7381 tggacgccaa cgccattcgc cgcgaggtcg ccagcgatcg ctacactggt gcgctgctgg
     7441 atcacagcgg cgggcatatt cacccgctga acctcgccat tggcgaagcg gacgctatcc
     7501 gcctgaacgg cgggcgcgtg tatgagcttt ccgcagtgac gcagatccag cacaccacgc
     7561 ccgccgtagt gcgtacagcc acaggtcagg tgacggcgaa gtatgtgatt gtcgccggga
     7621 atgcgtacct gggcgataaa gtggagccgg aactggcgaa acgcagcatg ccgtgcggta
     7681 cgcaggtgat caccaccgag cggctttccg atgatttagc ccgttcgctg atcccgaaaa
     7741 actactgcgt ggaagactgt aattatctgc ttgattacta ccgtcttacc gccgacaacc
     7801 gtttgctgta cggcggcggc gtggtgtacg gcgcgcgcga cccggatgac gttgagcgtc
     7861 ttgtggtgcc gaaactgctg aaaaccttcc cgcagttgaa gggcgtgaaa atcgactatc
     7921 gctggacggg caacttcctg ctgaccctgt cgcgtatgcc gcagttcggt cgcctcgata
     7981 ccaacatcta ttacatgcag ggttacagcg gccacggcgt cacctgtact catctcgccg
     8041 gacgtttgat tgccgaactg ctgcgcggcg acgccgaacg tttcgatgcc ttcgccaatc
     8101 tgccgcatta cccgttcccc ggcgggcgca cgctgcgcgt accgtttacc gcgatgggcg
     8161 cggcgtatta cagcctgcgc gatcgtctgg gcgtttaatt tccgattaac agtgaagagt
     8221 caaaaggtgt gaaacatgag caacaatgaa ttccatcagc gtcgtctttc tgccaccccg
     8281 cgcggggttg gcgtgatgtg taacttcttc gcccagtcgg ctgaaaacgc cacgctgaag
     8341 gatgttgagg gcaacgagta catcgatttc gccgcaggca ttgcggtgct gaataccgga
     8401 catcgccacc ctgatctggt cgcggcggtg gagcagcagc tgcaacagtt tacccacacc
     8461 gcgtatcaga ttgtgccata tgaaagctac gtcaccctgg cggagaaaat caacgccctt
     8521 gccccggtga gcgggcaggc caaaaccgcg ttcttcacca ccggtgcgga agcggtggaa
     8581 aacgcggtga aaatcgcccg cgcccatacc ggacgccctg gcgtgattgc gtttagcggc
     8641 ggcttccacg gtcgtacgta tatgactatg gcgttaactg gaaaggtcgc gccgtacaaa
     8701 atcggcttcg gcccgttccc tggttcggtg tatcacgtac cttatccgtc agatttacac
     8761 ggcatttcaa cacaggactc cctcgacgcc atcgaacgct tgtttaaatc agacatcgaa
     8821 gcgaagcagg tcgcggccat tattttcgaa ccggtgcagg gcgagggcgg tttcaacgtt
     8881 gcgccaaaag agctggttgc cgccattcgc cgcctgtgcg acgagcacgg tattgtgatg
     8941 attgccgatg aagtgcaaag cggctttgcg cgtaccggca aactgtttgc gatggatcat
     9001 tacgccgata agccggactt aatgacgatg gcgaaaagcc tcgcgggcgg gatgccgctt
     9061 tcgggtgtgg tcggtaacgc gaatattatg gacgcgcccg cgccgggcgg gcttggcggc
     9121 acctacgccg ggaatccgct ggcggtggct gccgcgcacg cggtgctcaa cattatcgac
     9181 aaagagtcac tctgtgagcg cgccatgcag ttggggcagc gtctgacgaa aaccctgatt
     9241 gatgccaaag aaaacgttcc ggccatcgcg gcggtacgtg gtctgggctc gatgattgcg
     9301 gcagaattta acgacccgca aacgggcgag ccgtcagccg ccattgcgca aaaaattcag
     9361 caacgcgcgc tggcgcaggg actgcttcta ctgacctgtg gcgcatacgg caacgtgatt
     9421 cgtttcctgt atccgctgac catcccggat gcgcaattcg atgcggcaat gaaaattttg
     9481 caggatgcgc tgagagatta atacgcagaa gcaggggcaa cgcaataatg cctgatatgc
     9541 tgcgcttatc agacctgcaa acgacgtatt gattatgtat gccggataag gcgttcacgc
     9601 cgcatccggc aagttgtatt gcccaacttc gctaaatctg gtgctttttc aacaacgcgc
     9661 ggaactgatg ataggttaag cccagcaatt cagctgcgcg cttctggtta tatttccctt
     9721 gttgcaaact gagttgcagc aactcttttt cctgctgcat ctgaaactca cgtaaatcca
     9781 gcggcagtgt tggaagcgag gtggtttctg aaacggcgat agcgtcttca ggcggacgcc
     9841 gtttaaaggg atcaataatg atgtcatcaa gcggataatc gctggtgccg tggcgataca
     9901 ctgaacgttc caccacgttt ttcaattcac gaatatttcc tggccaacga taattcagca
     9961 atgtttctct ggcgcgctcc gtaaaccccg ggaacagagg cagcttgatt tcccgacaca
    10021 tctggatggc aaagtgttcc gccattaaca taatgtcgct ttcacgttcg cgtagtggcg
    10081 gcagttgcac tacatcgaaa gccagccggt cgagcaggtc agcgcgaaaa gtaccttcat
    10141 tgaccatcgc cgggagatcg gcattcgtcg cgcataccaa ccgcacattc acctgcaacg
    10201 gctggctgcc gccaacgcgc tccagttcac cgtactcaat cacgcgcaat aatttctcct
    10261 gcaccatcat cggtgccgta gcgagttcat caagaaatag cgtaccgcca tcggcacgtt
    10321 caaatctccc tggatgacgt ttttgcgcac cggtaaacgc ccccgcttcg tgaccaaaca
    10381 gttcggaatc cagcagattt tcatttaacg ccgcgcagtt aagggaaata aacggccctt
    10441 gccaacggga ggagagataa tgcaggcggc tggcgatcag ctctttaccg gtgccgcgtt
    10501 cgccgatgat gagcaccggt ttgtccagcg gtgcgagatg cgaaacctgt tccagcactt
    10561 cgagaaagct gttcgcctca ccgagtaaat tatctttgta ttctgccatg atgaaattcg
    10621 ccacttgtta gtgtaattag ctaattcatc ctggcatgtt gctgttgatt cttcaatcag
    10681 atctttataa atcaaaaaga taaaaaattg gcacgcaaat tgtattaaca gttcagcagg
    10741 acaatcctga atgcagaaat caagaggaca acattatggg tattttttct cgctttgccg
    10801 acatcgtgaa tgccaacatc aacgctctgt tagagaaagc ggaagatcca cagaaactgg
    10861 tgcgtctgat gatccaggag atggaagata cattggttga agtacgttct acttcggcgc
    10921 gtgcgctggc agaaaagaaa cagctgactc gccgtattga acaagcgtcg gcgcgtgaag
    10981 ttgaatggca ggaaaaagcc gaactggcgc tgctgaaaga gagagaggat ttggcgcgtg
    11041 cggcgttaat tgaaaaacag aaactgaccg atctgattaa gtccctggaa catgaagtga
    11101 cgctggtgga cgatacgctg gcacgcatga agaaagagat tggcgagctg gaaaacaaat
    11161 tgagcgaaac acgcgctcgc cagcaggcat tgatgttacg ccatcaggcg gcaaactcgt
    11221 cgcgcgatgt gcgtcgtcag ctggacagtg gcaaactgga tgaagcaatg gctcgtttcg
    11281 aatctttcga acgtcgtatt gaccagatgg aagcggaagc agaaagccac agcttcggta
    11341 aacaaaaatc gctggacgat cagtttgccg aactgaaagc cgatgatgca atcagcgagc
    11401 aactggcaca attaaaagcc aaaatgaagc aagacaatca ataataacat ccaggcggcg
    11461 tccgaacgcg ccgccgctca tcgtctaagg agtacttatg agcgcgctat ttctggctat
    11521 tccattaacc atttttgtgc tgtttgtttt accgatctgg ttatggctgc attacagcaa
    11581 tcgttctggt cgcagtgaat tgtcgcaaag tgagcagcag cgattagcgc aactggctga
    11641 tgaagcaaaa cggatgcgcg aacgtattca ggcgctggaa tctattcttg atgcagaaca
    11701 tccgaactgg agggatcgct aatggcgggc attaatctca ataaaaaatt atggcgtatt
    11761 ccacagcagg gcatggtccg tggcgtctgc gccgggattg ccaactattt tgatgtaccg
    11821 gtaaaactgg tgcgtatcct ggtggtgttg tcgattttct tcggtctggc gctgtttacc
    11881 ctggttgctt acatcatttt gtcatttgcg cttgatccaa tgccggacaa catggccttt
    11941 ggtgagcagc taccttccag cagtgaattg ctggatgaag tcgaccgtga actggcggca
    12001 agtgaaacgc gtttacgcga gatggaacgt tatgtcactt ccgatacatt cacgttacgt
    12061 agccgtttcc gtcaactgtg aggaaagtta tgaatactcg ctggcaacag gccgggcaaa
    12121 aggtaaagcc tggtttcaaa ttagcaggca agctggtact tcttaccgca ctgcgctatg
    12181 gcccagcggg tgtggcgggc tgggcgataa aatcagttgc tcgccgaccg ctgaaaatgt
    12241 tgctggctgt ggcgctggaa ccgctgttaa gtcgggctgc caataaactg gcgcagcgtt
    12301 ataaaaggtg aggggagttt cgcaaaaatt gttaaatctc aggcgtataa tggatagcaa
    12361 tattcatcca tagaaggacg cttacatgtt taaaaaaggc ttacttgctc tggcactggt
    12421 gttttcactg cccgttttcg ccgctgaaca ctggatcgat gttcgtgttc cagagcagta
    12481 tcagcaagag cacgttcagg gggccatcaa tattcccctg aaggaagtga aagagcgcat
    12541 tgccaccgtc gttccggata aaaacgacac cgtgaaagtg tattgcaatg ccggacgcca
    12601 atcagggcag gcaaaagaga tccttagcga gatgggatat acccacgttg agaacgccgg
    12661 tggcctgaaa gacatcgcaa tgccgaaggt caaaggttaa aagaattcac catgagcggc
    12721 gcttatgcgc cgtttttttc tgtcacacct tatttacatc cctatagatt atttgcgtca
    12781 gctcacaaat acgctttttc cctggtaaaa aatgatttcc tgcgtgacta aaacccttgt
    12841 gctcaattga cagtttattt tctgcggagt agtctctcat ttcatgggac cgctaccagg
    12901 aaaggcaaca tgaaacagaa aattacggat tacctggacg aaatctacgg tggaacattt
    12961 accgcaactc atttacagaa acttgtaacg cgtcttgaga gtgcgaaacg attaattaca
    13021 cagcgacgta aaaaacactg ggatgaaagt gatgtcgtgt taattaccta tgccgatcaa
    13081 tttcacagta atgatttaaa accactaccc acatttaatc agttttacca tcaatggctg
    13141 caaagcattt tttcacatgt tcatttatta cctttttatc catggtcatc tgatgatgga
    13201 ttttcggtaa ttgattatca tcaggtcgcc agtgaagcgg gtagtggcag gatattcagc
    13261 agctcggtga atgcagtcat ttaatgtttg attttgtctg caaccatatg tcggcaaaaa
    13321 gtgaatggtt taaaaactat ttacaacagc agccaggttt tgaagatttt tttattgcgg
    13381 ttgatccgca aaccgatctc agcgctgtca cccgtccgcg tgcattaccg ctattaacgc
    13441 cgtttcaaat ggatgataat tccacgcgcc atttatggac cacctttagc gacgatcaaa
    13501 ttgacctgaa ttaccgtagc cctgacgtgt tgctggcgat ggtggatgtt ttgctttgtt
    13561 acctggaaaa gggcgcagaa tatgtccgtc tggatgccgt tggctttatg tggaaagagc
    13621 cgggaacaag ctgcatccat ctggaaaaaa cacatctgat tatcaaactg ttacggtcga
    13681 ttattgatga catcgcgcca ggtacggtga ttattactga gacgaatgtt ccgcataaag
    13741 ataacattgc ttactttggc gaaggcgatg acgaagcgca tatggtgtac cagttctcgc
    13801 tgccgccgct ggtgttacat gcggtgcaaa aacagaacgt cgaggcgctg tgccagtggg
    13861 cgcaaagtct gagcttgccc tccggtaaaa cgacctggtt taacttcctc gcctctcacg
    13921 atggcatcgg gctgaaccca ctgcggggct tgttaccaga aagcgaaata ttagcgctgg
    13981 tcgaggcatt acagcaggaa ggtgcattag taaactggaa aaataatccc gacggtacgc
    14041 gcagtcccta tgaaataaat gtcacctata tggatgcgtt aagccgccgc gagagtagcg
    14101 atgaagaacg ttgcgccagg tttatccttg cccatgcgat tttgttaagt ttccccggtg
    14161 tgccagcgat atatattcaa agtattctgg gctcgcgtaa tgattacgca ggtgtcgaaa
    14221 aactcggata taaccgtgcg attaaccgta aaaaatatca cagtgaagag ataacccgag
    14281 aactgaacga tgaagctaca ttaaggcatg cggtatatca tgagttgtcg cgtttaatta
    14341 cacttcgtcg cagccataac gagtttcatc cggataataa ttttaccatc gatacggtta
    14401 attcatccgt aatgcgtatt caaagaagta acgcagaggg taattgtctg accggattgt
    14461 ttaatgtcag taaaaatatt cagcatgtaa atattactgg cctgcacggt cgggatctga
    14521 ttagtgaagt tgatatattg ggtaataaaa taacgctgcg cccctggcag gttatgtgga
    14581 ttaaataaaa aggaacatct catgattaaa tcaaaaatcg tgctgttatc agcactggtt
    14641 tcctgcgccc tgatttcagg ctgtaaagaa gaaaataaac cccatgtatc catcgaattt
    14701 atgcattctt cggtggagca ggagcgtcag gcagttatca gcaaattgat tgcccgtttt
    14761 gaaaaagaaa acccaggtat caccgttaaa caagtgcccg tcgaagagga cgcttataac
    14821 accaaagtca tcactctttc acgtagcggc tcgctgccgg aagtgattga aaccagtcat
    14881 gactacgcca aagttatgga caaagagcag cttatcgatc gcaaggcggt tgccacggtc
    14941 atcagcaacg ttggtgaagg cgcgttttac gatggcgtac tgcgtattgt gcgtaccgaa
    15001 gatggtagcg catggaccgg tgttcctgtc agcgcctgga ttggcggcat ctggtatcgc
    15061 aaagatgtgc tggcaaaagc ggggctggag gagccgaaaa actggcaaca gctgctggac
    15121 gttgcacaga aactgaatga cccggcgaat aaaaaatacg gtattgcgct gcctacggca
    15181 gaaagcgtgc tgacggaaca atccttctcc cagtttgcgt tatccaacta ggccaacgtc
    15241 tttaacgccg aaggcaaaat cacccttgat tcaccagaga tgatgcaggc gctgacctat
    15301 taccgcgacc ttgctgccaa caccatgccg ggttctaacg acatcatgga agtgaaagac
    15361 gcctttatga acggcaccgc gccgatggcg atttactcca cctatatcct tccggctgtg
    15421 attaaagaag gcgatccgaa aaacgtcggt ttcgtggtgc caaccgagaa aaactctgcc
    15481 gtctacggca tgttgacctc gctgaccatt accgccggac aaaagactga agaaacggaa
    15541 gcggctgaaa aatttgtcac ctttatggag caggcagaca acattgccga ctgggtgatg
    15601 atgtcgccag gcgcggcgct gccggtgaac aaagcggttg tcactacttc cacctggaaa
    15661 gacaacgacg ttattaaggc gctgggtgaa ctgccaaatc agttaatcgg tgaactgccg
    15721 aatattcagg tctttggcgc agtaggggat aaaaaattta cccgcatggg cgatgtgacg
    15781 gggtctgggg tggtgagctc catggtgcat aacgtcaccg taggtaaagc cgatctccct
    15841 ggcacgctgc aaacgagcca gaaaaagctg gatgaactga tcgaacagca ctaaacccag
    15901 gacaggaatc cgcaatgaac aggctttttt caggtcgttc cgatatgccc tttgcgctgt
    15961 tgcttctcgc acccagctta ttactgctgg gcggtctggt tgcgtggcca atggtgtcga
    16021 atatcgaaat tagtttttta cgtctgccgc tcaatcccaa catcgagtca acgtttgttg
    16081 gggtaagcaa ctatgtgcgt attctctccg atcccggctt ctggcactcg ctgtggatga
    16141 cggtctggta taccgcgttg gtggtggcgg gcagcaccgt gctcgggctg gcagtggcga
    16201 tgtttttcaa ccgcgagttc cgcctgcgca aaaccgcgcg ttcgctggtg atcctctcct
    16261 acgtaacgcc gtctatttcg ctggtgttcg cctggaaata catgttcaac aacggctacg
    16321 gcattgtgaa ctacctcggc gtcgatcttc tgcatcttta tgagcaggca ccgctgtggt
    16381 tcgataatcc gggcagtagt tttgtgctgg tggtgatctt cgccatctgg cgctacttcc
    16441 cgtatgcctt tatctcgttt ctggcgattt tgcagaccat cgacaaatcg ctgtacgaag
    16501 cggcagagat ggatggcgct aatgcctggc aacggtttcg tatcgtcacg ctgcccgcga
    16561 ttatgccggt cctggcgacg gtggtgaccc ttcgcaccat ctggatgttc tacatgttcg
    16621 cggatgttta tctgctgaca accaaggtcg atattcttgg tgtctatctc tacaaaaccg
    16681 cctttgcctt taatgactta gggaaagcgg cggcgatctc ggtggtgctc ttcatcatta
    16741 ttttcgctgt cattctgctg accaggaaac gggtgaacct caatggcaac aaataaacgc
    16801 acactcagtc gtatcggttt ttaccgcggg ctggcgctgt ttctcatcat cacgctgttt
    16861 ccgttttttg tgatgctgat gacctcgttc aagagcgcaa aagaggcgat ctcgctgcat
    16921 cctacgctgc tgccgcaaca gtggacgctg gagcattatg tcgacatttt taacccggtg
    16981 attttcccgt ttgtcgacta cttccgtaac agcatggtgg tgtcggtggt ttcatccgtg
    17041 gtggcggtat ttctcggcat tctcggagct tacgcgcttt ctcgcctgcg ctttaaaggc
    17101 cggatgacca tcaacgccag cttttacacg gtgtatatgt tctccggcat tttgctggtg
    17161 gtgccgctgt tcaaaattat caccgcactt ggcatttacg acaccgagat ggcgctgatc
    17221 attaccatgg tgacgcaaac tctgcccacc gccgtgttta tgctgaaaag ttacttcgac
    17281 accatccctg atgagatcga agaagcggcg atgatggatg gcctcaaccg tctgcaaatt
    17341 atcttccgta tcaccgtgcc cctggcgatg tccgggctga tatccgtatt cgtctactgc
    17401 tttatggtgg catggaacga ctatctgttt gcgtcgattt tcctctccag cgccagcaat
    17461 ttcaccttac cagtcggcct gaacgcgctg ttcagtacgc cagattacat ctggggacgg
    17521 atgatggcgg cctcgctggt gaccgcattg ccggtggtga ttatgtatgc gctttcggaa
    17581 cgttttatta agagtggttt gaccgccggt ggcgtgaagg gctaaagcgg ccagtttctt
    17641 tacaaggagt tttacatgaa aaagttagta gccacagcac cgcgcgttgc tgcgctggtt
    17701 gagtatgaag atcgggcgat tttagccaat gaagtgaaga tccgcgtgcg tttcggtgca
    17761 ccgaaacacg gaacggaagt ggtcgacttc cgcgccgcca gcccgtttat cgatgaagac
    17821 tttaacggcg aatggcagat gttcaccccg cgtccggcag atgcgccgcg cggcattgag
    17881 tttggcaaat tccagcttgg caacatggtg gttggcgaca ttatcgagtg cggcagcgac
    17941 gttaccgact acgcggtggg cgacagcgta tgcggctacg gcccgctctc cgagacgatc
    18001 atcattaacg cagtgaataa ctacaagctg cgcaaaatgc cggaaggcag ctcctggaaa
    18061 aacgctgtct gctacgaccc ggcgcagttt gccatgagtg gcgttcgcga tgccaacgta
    18121 cgcgtagggg attttgtagt ggtggtaggg cttggcgcga tcggtcaaat tgccatccaa
    18181 ctggctaaac gcgctggcgc gtcggtggta attggcgtcg atcctattgc ccatcgttgt
    18241 gatattgctc gtcgtcacgg tgcggatttc tgccttaatc ccattggcac tgacgtaggc
    18301 aaagagatca aaacgctgac cggcaagcag ggtgccgatg tgattatcga aaccagcggt
    18361 tacgccgacg cgctgcagtc ggcgctgcgc ggcctggctt acggtggcac catctcctat
    18421 gtcgcgtttg ctaaaccgtt tgctgaaggt tttaacctcg gacgcgaagc gcatttcaat
    18481 aacgccaaaa ttgtcttctc tcgcgcgtgc agtgaaccga acccggatta tccgcgctgg
    18541 agccgtaagc gtattgaaga aacctgctgg aaactgctga tgaacggtta tctcaattgc
    18601 gaagatttaa tcgacccggt agtgaccttt gccaacagcc cggaaagcta catgcagtat
    18661 gtcgatcagc atccggaaca gagcatcaaa atgggcgtca cgttttaatt caaaggaatg
    18721 aaaaaatgaa aataggcaca cagaatcagg cgttcttccc ggaaaatatt ctggagaaat
    18781 ttcgttatat caaagagatg ggcttcgatg gttttgagat tgacggcaaa ctgctggtca
    18841 acaacatcga agaagtcaaa tcggcgatca aagaaaccgg tttaccggtg accaccgcct
    18901 gcggcggcta tgacggctgg attggtgact ttatcgaaga gcgtcgtctt aatggcttaa
    18961 agcagatcga acgcattctc gaagcgctgg cagaagtggg gggtaaaggt atcgtcgttc
    19021 cggcagcgtg gggcatgttt accttccgct taccgccgat gacctcgccg cgtagcctgg
    19081 acggcgaccg caaaatggtg agtgattccc tgcgcgtact ggaacaggtc gccgcgcgta
    19141 ccggaaccgt ggtgtatctc gaaccgttaa accgctatca ggatcatatg atcaacaccc
    19201 tcgccgatgc tcgtcgttac agcgtcgaaa acaatcttaa acatgtgcag attatcggcg
    19261 atttctatca catgaacatc gaagaagata acctggcgca ggcgctgcat gacaaccgcg
    19321 acttgctcgg tcatgtgcat attgccgata accatcgtta ccagccgggc agcggcaccc
    19381 tcgatttcca cgcgctgttt gaacagctgc gcgccgataa ctatcagggt tatgtggtgt
    19441 atgaagggcg tatccgggca gaagatcctg cccaggcgta ccgtgattcg ttgacctggt
    19501 tgcgtacctg ctaagaggtc tttgtgaaaa gtgcaatgac aagctctcca ctgcgggtcg
    19561 cgataatagg cgcaggccag gtggcggata aggttcatgc ttcgtactac tgcacccgca
    19621 acgatctgga actggtggct gtctgtgaca gccgcctttc ccaggcgcag gcgctggcag
    19681 aaaaatacgg gaatgcatcc gtgtgggacg atccgcaggc catgctgctg gcggtgaaac
    19741 ctgatgtggt tagcgtctgc tcacctaacc gttttcatta cgaacatacc ctgatggcgc
    19801 tggaagcggg ctgccatgtg atgtgcgaaa aaccgcccgc catgacgcca gaacaggcgc
    19861 gggaaatgtg cgataccgcg cgcaaacagg gcaaggtgct ggcctacgac tttcaccatc
    19921 gttttgcgct cgatacgcaa cagctgcgtg aacaggtgac caacggcgtt ttgggagaga
    19981 tttacgttac caccgcccgc gccctgcgtc gctgcggcgt tcccggctgg ggtgtcttta
    20041 ccaataaaga actgcagggt ggtggcccgc tgatcgacat cggcattcat atgctggatg
    20101 ctgcgatgta tgtgctgggc tatcctgcgg tgaaaagcgt gaatgcgcat agctttcaaa
    20161 agatcggcac gcaaaagagc tgtggtcaat ttggcgagtg ggatccggca acttacagcg
    20221 tcgaagattc gctgtttggc accattgaat ttcataacgg cggcattctg tggctggaaa
    20281 cgtcatttgc gctcaacatc cgcgaacagt cgattatgaa cgtcagcttt tgtggtgata
    20341 aagccggtgc gacgctgttt ccagcacata tctacaccga taacaacggt gaattaatga
    20401 cgctgatgca acgggaaatg gcagacgaca accgccattt gggcagcatg gaagccttta
    20461 tcaatcacgt acagggcaag cccgtgatga tagccgacgc cgagcagggg tacatcatcc
    20521 agcaactggt ggcggcgtta tatcaatccg cagaaacagg gacgcgtgtg gaattatgac
    20581 caggccagta acgttatcag aaccccattt cagccagcat accctgaaca agtatgcatc
    20641 gctgatggcg caggggaacg gctatcttgg gcttcgcgcc agccatgaag aagattacac
    20701 gcgccagacg cgagggatgt atctggcggg gctgtatcat cgggcgggaa aaggtgaaat
    20761 caacgaactg gtgaacctgc ctgatatctt ggggatggag attgccataa atggtgaggt
    20821 tttctcgtta tcccacgaag cctggcagcg tgagcttgac tttgccagtg gcgaattacg
    20881 ccgcaacgtt gtctggcgta ccagcaacgg cgcaggttac accatcgcca gccgtcgctt
    20941 tgtttcggca gaccaactgc cgctcattgc gctggaaatc actattacgc cactggacgc
    21001 cgacgcgtca gtgctgattt caacaggtat cgacgccacg caaaccaacc acggtcgcca
    21061 acatctcgac gaaacccagg tgcgggtgtt tggtcagcat ctgatgcagg ggatctacac
    21121 cacccaggat ggacgcagtg atgtggccat cagctgttgc tgtaaggtga gcggtgatgt
    21181 gcagcaatgc tataccgcca aagagcgccg tttgctgcaa cataccagtg cgcagcttca
    21241 tgcaggcgag acagtgacgt tgcaaaaact ggtgtggatc gactggcggg atgacaggca
    21301 agccgtttta gacgagtggg gcagcgcgtc gcttcgccag cttgaaatat gcgcgcagca
    21361 gagttacgac caacttcttg cagcatcaac agaaaactgg cgtcaatggt ggcagaaacg
    21421 tcgtatcacg gtaaatggcg gcgatgcgca cgatcagcaa gcgttagatt atgcgcttta
    21481 tcatctgcgc atcatgacgc cggctcacga cgagcgcagc agtattgcgg caaaaggctt
    21541 aaccggcgaa ggctacaaag gccacgtttt ctgggataca gaagtatttt tgctgccgtt
    21601 ccatctgttt agcgatccga cggttgcccg aagtttactg cgttatcgct ggcacaactt
    21661 gccaggcgcg caggagaaag cacggcgcag cggctggcag ggcgcgctat ttccgtggga
    21721 aagcgcgcgc agcggcgaag aagagacgcc agaatttgcc gccattaaca ttcgtaccgg
    21781 gctgcggcaa aaagtggcct cggcgcaggc ggaacatcat ctggtggccg atatcgcctg
    21841 ggcggttatt caatactggc agaccacggg ggatgaaagt ttcattgctc atgaaggcat
    21901 ggcgctactt ctggaaactg caaagttctg gattagccgc gcggtgaggg ttaacgaccg
    21961 tctggaaatt catgatgtta ttgggccaga cgaatatacc gaacatgtca ataataacgc
    22021 cttcaccagc tatatggccc gctacaacgt tcaacaggcg ctgaatattg cccgccagtt
    22081 cggctgtagc gacgatgcgt ttatccatcg cgccgaaatg ttcctcaaag agctatggat
    22141 gccagaaacg cagcccgatg gcgttttgcc gcaggatgat tcgtttatgg ctaagccggc
    22201 gattaatctg gctaaataca aagcggcggc ggggaagcaa accattctgc tggattattc
    22261 acgcgcagaa gtgaacgaga tgcagatcct caaacaagct gatgtggtga tgctcaatta
    22321 catgctgccg gagcagttct cagcggtatc gtgtcttgcc aatctgcaat tttatgaacc
    22381 gcgcactatt cacgactcgt cattaagtaa agcaatccac ggcattgttg ccgcacgctg
    22441 tggcctgctg acccaaagtt atcagttctg gcgcgagggg actgaaatcg atcttggtgc
    22501 tgatccgcat agttgtgatg atggtatcca cgctgccgca actggcgcta tctggctggg
    22561 ggcgattcag ggttttgccg gggtgagcgt gcgtgacggt gaattacatc tcaatccggc
    22621 gttaccggag cagtggcaac agttgtcttt ccctctgttc tggcagggct gcgaattaca
    22681 ggtcacgctc gacgcgcagc gtattgcgat tcgaacttct gcgcccgttt cactgcgttt
    22741 gaacgggcag cttatatccg tggctgaaga atctgttttc tgtttgggtg attttatttt
    22801 gcccttcaat gggaccgcta ccacgcatca ggaggatgaa tgaaactgca aggggtaatt
    22861 ttcgatctgg atggtgtaat caccgatacc gcgcatctgc atttccaggc gtggcagcag
    22921 attgccgctg aaattggcat cagcattgat gcgcagttta acgaatccct aaaagggatc
    22981 agccgcgatg agtctctgcg gcgcattctg caacacgggg gcaaagaggg cgactttaac
    23041 ccgcaggagc ggacgcaact ggcgtatcgc aaaaatctgc tttacgtcca ctcactgcgc
    23101 gagctgacgg ttaatgctgt gctgcccggt attcgcaatt tgctggcaga gctccgcgca
    23161 cagcagatcc cggtagggct ggcttctgtc tccctgaatg cgccgacgat tttagcggcg
    23221 ctggagctgc gcgagttttt caccttctgc gcggatgctt cccaacttaa aaactcgaaa
    23281 ccggatccgg aaatctttct cgctgcctgt gccgggctgg gcgtgccgcc gcaggcatgt
    23341 atcggcattg aagatgcgca ggcgggcatt gacgccatta acgccagcgg tatgcgctcg
    23401 gtggggatcg gcgcaggctt aaccggggcg caattattgt tgccctcaac ggactccctg
    23461 acctggccgc ggttatcggc cttctggcaa aacgtatagc aaaggaatca acatggctca
    23521 gctttcgtta caacatattc aaaaaatcta cgataaccag gtgcatgtgg tgaaggactt
    23581 caacctggaa attgccgata aagagttcat cgtatttgtc ggcccgtcgg gctgcggtaa
    23641 atcgaccacc ctgcgcatga ttgccgggct tgaggagatc agcggcggcg atctgttgat
    23701 cgacggcaaa cgaatgaatg acgttccagc caaagcacgc aatatcgcaa tggtgttcca
    23761 gaactacgcg ttgtatccgc atatgacggt ttacgacaac atggcgtttg gcctgaagat
    23821 gcaaaaaatc gccaaagagg tgattgatga gcgggtgaac tgggcggcgc aaattctcgg
    23881 cctgcgtgag tacctgaagc gcaagccggg cgcgctttcc ggcgggcaac gccagcgtgt
    23941 ggcgctcggg cgggcgattg tgcgcgaagc gggcgtgttt ttaatggatg aaccgctctc
    24001 taaccttgat gccaagctgc gcgtacaaat gcgcgcagag atcagcaagc tgcatcagaa
    24061 actgaacacc accatgatct acgtgaccca cgatcagacc gaagcgatga ccatggcgac
    24121 gcggattgtg attatgaaag acgggattgt tcagcaagtg ggtgcgccga aaaccgttta
    24181 taaccaaccc gcgaatatgt ttgttgccgg atttattgga tcaccagcga tgaattttat
    24241 tcgcggcacg atcgatggcg ataaattcgt tacggaaacg cttaaattaa ccattcccga
    24301 agagaaatta gcggttctga aaacacagga aagtttgcat aagcccatcg tgatgggaat
    24361 gcgcccggaa gatattcatc cggacgcgca agaggaaaat aatatttccg ccaaaattag
    24421 cgtggtagaa ttaaccggtg cggaatttat gctctacacc acggttgggg ggcacgagtt
    24481 agtggtccgt gctggtgcgt taaatgatta tcatgcagga gaaaatatca ctattcattt
    24541 tgatatgact aaatgtcatt tctttgatgc agaaacggaa atagcaattc gctaaataca
    24601 gggggaaggc attcccccag gataatacaa ggaacaataa tgaaaaagtt attaccctgt
    24661 accgcactgg tgatgtgtgc gggaatggcc tgcgcacagg ccgaggaaaa gaacgactgg
    24721 cactttaata tcggcgcgat gtacgaaata gaaaacgtcg agggttatgg cgaagatatg
    24781 gatgggctgg cggagccttc tgtctatttt aatgccgcca acgggccgtg gagaatttct
    24841 ctggcctatt atcaggaagg gccggtagat tatagcgcgg gtaaacgtgg aacgtggttt
    24901 gatcgcccgg agctggaggt acattatcag ttcctcgaaa gcgatgattt cagtttcggc
    24961 ctgaccggcg gtttccgtaa ttatggttat cactacgttg atgaaccggg taaagacacg
    25021 gcgaatatgc agcgctggaa aatcgcgcca gactgggatg tgaaactgac tgacgattta
    25081 cgtttcaacg gttggttgtc gatgtataaa tttgccaacg atttgaacac gaccggttac
    25141 gctgataccc gtgtcgaaac ggaaacaggt ctgcaatata ccttcaacga aacggttgcc
    25201 ttgcgagtga actattatct cgagcgcggc ttcaatatgg acgacagccg taataacggt
    25261 gagttttcca cgcaagaaat tcgcgcctat ttgccgctga cgctcggcaa ccactcggtg
    25321 acgccgtata cgcgcattgg gctggatcgc tggagtaact gggactggca ggatgatatt
    25381 gaacgtgaag gccatgattt taaccgtgtt ggtttatttt acggttatga tttccagaat
    25441 ggtctttcca tttcgctgga atacgcgttt gagtggcagg atcacgacga aggcgacagc
    25501 gataaattcc attatgcagg tgtcggcgta aattactcgt tctgataatg ggctaaattg
    25561 ccggatgcgg cgcgagtact ttatccgatc tataaatgta ggccggataa gatgcgccag
    25621 catcgcatcc ggcattcagg caaggtcgct ggtatttatt tcagcgtcat atgcgtggca
    25681 acggtaatat tttgtggtga cggttttccg gcaattaagc ggaataataa ctcacagctt
    25741 tgtcgaccta actcctgcgt cggaacatcg atgccgcccg gtgcaggcgt taaaataaat
    25801 gacagcgttt cattgctata acccaccacc gctaactgct gcggaatagc aatgtttttc
    25861 tctgccgccg cacgataaat gctcattaat ttcaggctgt cagtggcaaa cactgcctca
    25921 ggcaacggtg actgacttaa taattgccgt gctgcttgca gtgctgtttc atgggtataa
    25981 ccgccatcaa caatccattc atcacgcact gcaatattat gatcttccag actctgctta
    26041 taaccattta cccgatccac tgaaacatgg acatcaagcg gtgcatgcag acaggcaata
    26101 ttttgatgtc cactttcaat taacgcatcg gtcaacgcaa tgctgtcacc aaaattatcg
    26161 gtatcgacag aataaacatg ggcatattga ccttcaactt tgccaatcac caccacagga
    26221 atatcgtatt tatcaagttg ggcaaaaaat gactcatccg ctggcgaact tagcataata
    26281 atgcctttaa tcattttctg cttaattttg ctttcacatt tttgtaagtc ttctgccggg
    26341 gtgtgcgacg tctgcaatat cacgtcgaaa ccttcttctt cagctttggc ggtgatggca
    26401 tgcaaaactt cagagaaaaa cggattaccc gccgtagttt tggtcgaacg ggtagaaatc
    26461 accataatgg catcaaaacc cgaagaggtc agcgcgcggg ccagcttgtt tggttgatac
    26521 tgtaattctt caatggcccg taacactttt tcgcgcgctt ccggggagat attggtttgc
    26581 ttattcagca cgcgtgatac ggtggatttt gatacgcctg caaccctggc aatatcataa
    26641 atagtagggg acataggtca ggaactccgt ccgaacgtga atgctgcaca tcttatggag
    26701 ttcgcccgtc gatgacaaca cgggtagttg ataatcaatg gcctggcccc cacattcata
    26761 tccttatgaa tgattttttt ctggtcttca gagcagcaca ggacagcaat gaagcgactt
    26821 aaaaatgaac ttaatgcgct ggtgaatcgg ggtgtcgaca gacatctgcg cctcgccgta
    26881 accggactta gccgcagcgg caaaacggcg tttatcactg cgatggtcaa tcagttgctc
    26941 aatattcatg ccggagcacg tttgccgcta ttaagtgcgg tgcgtgaaga gcgtctgctg
    27001 ggcgtaaaac gcattcctca gcgtgacttt ggcattccgc gttttaccta cgacgaaggg
    27061 ttggcgcagc tgtatggcga tcctcccgcc tggccgacgc caacgcgcgg cgtcagcgaa
    27121 attcgcctgg cactacgcta taaatcgaac gattcgctgc tgcgccactt taaggatacc
    27181 tccacgctgt atctggagat tgtggattac cctggcgaat ggttgctcga cctgccgatg
    27241 ctggcgcagg actatttaag ctggtcgcgc cagatgacgg gcttactcaa tggtcagcgc
    27301 ggcgaatggt cggcgaaatg gcgaatgatg tgcgaagggc tggacccgct agcgcctgcc
    27361 gacgaaaacc ggctggcaga cattgccgcc gcgtggaccg attatctcca ccactgtaaa
    27421 cagcaggggc tgcactttat tcagcctggg cgctttgtct tgccggggga tatggcaggt
    27481 gcgcccgcgc tgcaattctt cccgtggccg gatgtcgatg cctggggcga gtccaaactg
    27541 gcgcaggccg ataagcacac caatgccgga atgctgcgcg agcggtttaa ttattactgc
    27601 gagaaggtgg tgaaggggtt ctataagaat cattttctgc gctttgaccg ccagattgtg
    27661 ctggtggatt gcctgcaacc tctcaacagt gggccacagg catttaatga tatgcgtctg
    27721 gcgctgacgc agctgatgca aagtttccac tatgggcagc gtaccctgtt caggcgtttg
    27781 ttttcgccgg ttatcgataa gctattgttt gctgccacta aagcggacca tgtgaccatc
    27841 gatcagcacg ccaatatggt ttcattgctg caacaactaa ttcaggatgc ctggcaaaat
    27901 gcggcgttcg aagggatcag catggattgc ctggggctgg cgtcagttca ggcgaccacc
    27961 agtggcatta ttgatgttaa cggtgagaaa atcccggcgc tgcgcggtaa tcgacttagc
    28021 gatggcgcac cgctcactgt ttatcctggc gaagttcccg cacgtttgcc tggtcaggcg
    28081 ttctgggata agcaagggtt ccagtttgaa gcgtttcgcc cgcaggtgat ggatgtcgac
    28141 aaaccgctgc cgcatattcg tcttgatgcc gcgctggaat ttttaatagg agataaattg
    28201 cgatgaccga accgttaaaa ccacgtattg atttcgacgg tccgctggag gtcgaacaga
    28261 atccaaaatt cagggcgcag cagacctttg acgaaaatca ggcgcaaaat tttgccccgg
    28321 ccacgctcga cgaagcgcag gaagaagagg ggcaagtcga agcggtaatg gacgcagcgt
    28381 tacgtccgaa acgcagcctg tggcgcaaaa tggtgatggg cgggctggct ctgtttggcg
    28441 caagcgttgt cgggcagggt gtacagtgga caatgaatgc ctggcaaact caggactggg
    28501 tggcgctggg tggatgtgct gctggggcat tgattatcgg cgctggcgta ggttctgtgg
    28561 taacagagtg gcggcgctta tggcgcttgc gacagcgcgc ccatgaacgc gacgaagcgc
    28621 gcgatttgtt gcacagccac ggcacgggca aaggccgcgc attttgcgaa aaactggcgc
    28681 agcaggcggg tattgatcag tctcatccag cgctgcaacg ctggtatgcc tcaatccatg
    28741 aaacgcagaa cgatcgtgaa gtggtcagcc tgtatgctca tctggtccag ccggttttag
    28801 atgcccaggc gcggcgcgaa atcagccgct cagcagctga atcaacgttg atgattgcgg
    28861 tcagcccgct ggcgctggtg gatatggcat ttatcgcctg gcgcaatctg cgtttgatta
    28921 atcgcatcgc cacgctgtat ggcattgaac tgggatatta cagccgtttg cgcctgttca
    28981 agctggtatt gctgaatatc gctttcgccg gagccagcga attggtgcgc gaagtgggaa
    29041 tggactggat gtcgcaagat ctcgctgctc gtttgtctac ccgcgcagct caggggattg
    29101 gtgcaggact tctgacggca cgactgggga ttaaagctat ggagctttgc cgcccgctgc
    29161 cgtggattga cgatgacaaa cctcgcctcg gggattttcg tcgtcagctt atcggtcagg
    29221 tgaaagaaac gctgcaaaaa ggcaaaacgc ccagcgaaaa ataatgcaat atcgggtgct
    29281 gaccggatat ctttacgccg aagtgcccgt ttttccgtct ttgtgtcaat gattgttgac
    29341 agaaaccttc ctgctattca aatagtgtca tatcatcata ttaattgttc ttttttcagg
    29401 tgaaggttcc catgcgtctg gaagtctttt gtgaagaccg actcggtctg acccgcgaat
    29461 tactcgatct actcgtgcta agaggcattg atttacgcgg tattgagatt gatcccattg
    29521 ggcgaatcta cctcaatttt gctgaactgg agtttgagag tttcagcagt ctgatggccg
    29581 aaatacgccg tattgcaggt gttaccgatg tgcgtactgt cccgtggatg ccttccgaac
    29641 gtgagcatct ggcgttgagc gcgttactgg aggcgttgcc tgaacctgtg ctctctgtcg
    29701 atatgaaaag caaagtggat atggcgaacc cggcgagctg tcagcttttt gggcaaaaat
    29761 tggatcgact gcgcaaccat accgccgcac aattgattaa cggctttaat tttttacgtt
    29821 ggctggaaag cgaaccgcaa gattcccata acgagcatgt tgttattaat ggacagaact
    29881 tcctgatgga gattacgcct gtttatcttc aggatgaaaa tgatcaacac gtcctgacag
    29941 gtgcggtggt gatgttgcga tcaacgattc gtatgggccg tcagttgcaa aatgtcgccg
    30001 cccaggacgt cagcgccttc agtcaaattg tcgctgtcag cccgaaaatg aagcatgttg
    30061 tcgaacaggc gcagaaactg gcgatgctaa gcgcgccgct gctgattacg ggtgacacag
    30121 gtacaggtaa agatcttttt gcctacgcct gccatcaggc aagcccaaga gcgagcaaac
    30181 cttacctggc gctgaactgt gcgtctatac cggaagatgc ggtcgagagt gagctgtttg
    30241 gtcatgctcc ggaagggaag aaaggattct ttgagcaggc gaacggtggt tcggtgctgt
    30301 tggatgaaat aggtgagatg tcaccacgga tgcaggcgaa attactgcgt ttccttaatg
    30361 atggcacttt ccgtcgggtt ggcgaagacc atgaggtgca tgtcgatgtg cgggtgattt
    30421 gcgctacgca gaagaatctg gtcgaactgg tgcaaaaagg catgttccgt gaagatctct
    30481 attatcgtct gaacgtgttg acgctcaacc tgccgccgct acgtgactgt ccgcaggaca
    30541 tcatgccgtt aactgagctg ttcgtcgccc gctttgccga cgagcagggc gtgccgcgtc
    30601 cgaaactggc cgctgacctg aatactgtac ttacgcgtta tgcgtggccg ggaaatgtgc
    30661 ggcagttaaa gaacgctatc tatcgcgcac tgacacaact ggacggttat gagctgcgtc
    30721 cacaggatat tttgttgccg gattatgacg ccgcaacggt agccgtgggc gaagatgcga
    30781 tggaaggttc gctggacgaa atcaccagcc gttttgaacg ctcggtatta acccagcttt
    30841 atcgcaatta tcccagcacg cgcaaactgg caaaacgtct cggcgtttca cataccgcga
    30901 ttgccaataa gttgcgggaa tatggcttaa gccagaagaa gaacgaagag taagcgcgaa
    30961 tatgcctgat ggtgcaacac catcaggcat attaacttat gctttcagta cagccagagc
    31021 tgcttcgtaa tccggctcgg tggtgatttc atccaccagc tggctgaaaa tcacattgtc
    31081 attttcatca ataaccacaa cggcacgcgc tgccagacct ttcagtgggc catcagcaat
    31141 tgccacaccg taggcttgca gaaattcagc gttacggaaa gtggagaggg tgataacgtt
    31201 gttcagacct tctgcgccgc agaaacgaga ctgggcgaac ggcagatcgg cagagataca
    31261 cagcacaacg gtgttgtcga tctcggttgc cagttggtta aacttacgta ctgatgcggc
    31321 gcaaacaccg gtatcaatac tcgggaaaat gttcagcact ttgcgtttac ccgcaaactg
    31381 accgagggtg acgtcagaca gatcttttgc cacgagagta aaagtctgcg ctttgctacc
    31441 cgcctgcggg atggaattgg cgactgtaac cgggttgccc tggaaatgaa cggtttgtga
    31501 catgattatc tttcctgttt acatatagtt aacgtcacac ctagtttatg ctaactgtca
    31561 ataacacagc aaacgctatt tgcgcttaat ccgcagacca ccgcgacacc aaggagtaaa
    31621 gatgagaacc gttaaggtat ttgaggaagc ctggccctta cataccccgt ttgtgattgc
    31681 ccggggaagt cgcagtgaag cgcgcgtggt ggtggttgaa ctggaagaag agggtattaa
    31741 aggcaccggc gaatgcacgc cgtatccgcg ttatggagaa agtgatgcct cggtaatggc
    31801 gcaaattatg agcgtcgtgc cgcaattaga gaaagggctg acacgggagg agttgcaaaa
    31861 aattctccct gccggcgcag cacgtaatgc gctggattgt gcattgtggg atctggccgc
    31921 gcgaaaacag cagcaatcgc tggctgattt gatcggcata acgcttcccg agacagttat
    31981 cactgcacag acggtagtca tcggtacgcc tgatcagatg gccaatagtg catcaacact
    32041 ctggcaggcc ggcgcgaaat tactgaaagt gaagctggat aaccatctta tcagtgagcg
    32101 gatggtggca attcgcacag ctgtgcccga taccacgctg atcgttgatg caaatgaatc
    32161 ctggcatgca gaagggttgg cggcacgttg ccagctattg gcggatttag gcgttgcgat
    32221 gcttgaacaa ccgcttcctg cgcaggacga tgcggcgctg gagaatttta ttcatccgtt
    32281 gccgatttgt gctgatgaaa gttgtcatac tcgtagcaat ttgaaggcgc tgaaagggcg
    32341 ctatgagatg gttaatatta agctcgataa aaccgggggt ctgacggaag cgctggcgct
    32401 ggcgactgaa gcgcgtgcac aaggtttcag tctgatgctg ggctgcatgt tgtgtacctc
    32461 tcgggccatt agcgccgctt taccgctggt gccgcaggtc agtttcgccg atcttgacgg
    32521 accgacctgg ctggcggtag atgtggaacc ggcgcttcag ttcacgacgg gcgaattgca
    32581 tctttaggat gccagcgcag caagtttgcc atcgcaaaga gatatttttc gctggcttcg
    32641 tcggaggaga tgggcggaaa ctctgcggtg atgcaatgca agttcagatc tgcacaccaa
    32701 ctgccgaaag agccaggcgt ttcataaccg acgctggtta ccagaggcaa ttcaaacgcc
    32761 tgagccagcc attcgcctaa ttcgctgtgt ctgggatctt caatacaggc cagtggatcg
    32821 tggaatgaga ccacccaggc aggttgaatg cggtgtataa gctggcacag cgcctgggtt
    32881 tcaggttcgg agccaggttt gtcacctgtc agtaatacga catcacgttc ttcagcggcg
    32941 ctgttccagc gataaaccgt ttcaccttcc ttccagttcg ccgccggaaa gtttctgttt
    33001 aaatccacac cattagcatt ggcccgtaac cccagctggc agccgtcagg attcacacac
    33061 agcaccacat gatggcggcg caaagaaggt gtcaaagtcc gcagagcaca ggagagcgtg
    33121 acgaccgaag agttttcatc accgtgagtg ccagccagaa tcaaaccact ttcgcgactg
    33181 gctgcagggg ccggaaacca gattaacggc gcgcccaata atgaacgtcc gtaatgttct
    33241 gttccgggcg gaaatgcgcc gcgttcggcg cgtgggcggg ttacggtcat gatcgtctct
    33301 ggttcaggaa gtattacagg cagtgttgtg caaattttgc ggattatcaa atccttttct
    33361 tcaacgctat gtcatcgatc ttgtatcact tttttgccgg aagttgtttg tattttcatt
    33421 gatgtgcaga tattagaatt gtcgtaatgt tatatacatg gcgtatttta ttcttactgg
    33481 ttctacacat gttaatcgca tctgattgat cagttcacct taataagttt aataaatcgt
    33541 actgcacata gtacagtacg atggtggtta tataattgat ttacatgaag aatggcttgt
    33601 cgccatcagt cccgggttga ttgatgccta tattctcacc aatgtgtttc tcaggtttat
    33661 cgatgatatc tgtaattaat gctgcgacac tcttaggtga tacgatcgta cctttgaatg
    33721 gttcattgcg tgaggtaagc tcataatcaa taatatcttc atccgtcagc caggcagggc
    33781 gaagaattgt atattccagt ccagaagcct caattgcatc cgcagccctg cggaatggtt
    33841 ttaggggctc accaataact gcattattcc attcgccaaa ttttcctgga acttcatcat
    33901 aaatgccgag ggaaagaaca aagatcagac gtttaacatc acaggctttc atcgcagcga
    33961 tcactgaatt tgcctgaata tctaaatctt cccccgtaag gttggcatac acaatatcct
    34021 gtccctgcat ggcctgcttc agtgctgcat gattcagtac atcccccata ataatctggc
    34081 tattagttgg atagggtttg tgtatttttg ctggctgacg agcaaataaa gtttgtttaa
    34141 tggtttgttt atctgccagt tgattaatca catggcgggc tatctgaccg ccagcaccaa
    34201 gaatgagtac atttttcata atatttctca tgaaacttat cacagatggg tgttgaaaaa
    34261 aggaataact ttttccagcg cctcctgaac tgcttccggt ttgtcataca gttcataatg
    34321 tgagaaatcg ggtaatgaaa ctaactgacg atcctgcgaa accatcgccc ggccatagac
    34381 ttccagtcca tcacgataag caccgaaggc accgactttt tcaccaacta ctaccatcac
    34441 cggctgagtc agtaacactt cagtaaatgc gaaggcatcc caggccagcg ttttctgtgc
    34501 atgtgagaac aacatttttg tcgcacctcc cggttgttgc ccgcgtggtg ttttatagta
    34561 gtcagttgct tcataaacat cgcgttcggt cagtccatgg gcttttgctg catccaggct
    34621 ggcgggaagt aattcgttga tctgtaattc tccaccacga gcctctttgg ttcgttgcga
    34681 cgccattgca ttcaatgcgc caatcggatc gtaattgctg aaaccttccc gaaaaagacg
    34741 cccgatatta acacccgtaa tactcactac agctttaatt cgtttttctg tcagcgcggc
    34801 attaatggca tagccacccc cccccacaca cacccagcac gccaatgcgt tcagcatcaa
    34861 catatggcag agttactgca taatcgataa cacggctaat atcctctacg cgctgtgtcg
    34921 gatcttctat ccaacgaggc tccccaccag attctccctg gaaactggcg tcataagcga
    34981 gaacgacata gcctttttca gccaatgcct tgccatagat attgccagac gtctgctcct
    35041 tacaactgcc aaaaggatgg acgctgataa tcgccggata ctgttttgac tcatcaaaat
    35101 ttggtggaaa ctgaatattg gcagcaatat gccaggccat atcactattg cggatttgaa
    35161 tatgtttcat tacagagttt cctcaaagaa cggagctaat actgaaattg cttcagccac
    35221 ataggctttg ccgtcataaa gatccatatg gtttgcgcct tcaacaatgt gatagcgttt
    35281 atcctggctt gatgcccgat cgtacaggtc gtcactcatc catttgctcc ctgcctggct
    35341 gcccgccaca atctgcgtcg gctgagtcag gtacacttcc gccatatggt aagcatcata
    35401 ggtaataatc tggttaaggc tgcgcaaagt agcgtaaccc ggtgctgttg gatactgcgc
    35461 gcgaggggtg tggtaatact cccaggcctg acgcagttct tcgttcggcg catcggactc
    35521 cttcattggt gccagtggca taatggcgta ttctccgctg ctaatatcgc tggttctggc
    35581 gtttgaaccc gcttcaacgt atggcagggc atcaatagat ttcacattgt tttcccaacc
    35641 attacggaac atcgaaccaa tattgaccgc actaacggta ccgatggcct tgatgcggcg
    35701 atcctgaatt gcagcattgg ctgtatatcc tgcaccggca caaattccca tcgcaccaat
    35761 tcgggtattg tcgacataag aaagcgttgt caggtaatca atcacggcac tgacgtcttc
    35821 agtacgaatg tatgggtttt ctaactgacg cggctcgccg ccactttcac cctgataaga
    35881 tgcgtcataa gcaatagtga caaatccctt ttccgccagt ttttcggcat aggtcccggc
    35941 cgtttgttct ttaacgcccc cacctgggtg agataccaca attgccggat attgacgggt
    36001 ttcatcaaat tttggaggaa aatagatcac tgcagacaaa gagatggttg gattattgct
    36061 gttagtgaag ctgactttat tattcatcat tccgttcctc atggagttgt tggttcgttt
    36121 taacggttgg tgatatcact atagatattg atcattaagt tgattagacc ctaaatcatg
    36181 attagaccta tcatttaaat gattaataga ttgtatggag atactgaatg aagcgtgaag
    36241 agatcgctga tctgatggcg tttgtcgtcg ttgcagagga gcgtagcttc actcgcgcag
    36301 cagcccgcct gagcatggcg cagtcagctt taagccagat agtgcgccgt atagaagaac
    36361 gattgggatt gcggcttctg acgcgaacca cgcgcagtgt tgtacccact gaagcgggcg
    36421 agcatctttt gtctgttctt ggccctatgc tgcatgacat agattctgcc ctggcatccc
    36481 tgagcgatct gcagaaccgc ccatccggga caatacgtat tactactgta gaacatgcag
    36541 caaaaacgat attgttacca gccatgcgca cattcctgaa atcacaccct gaaattgata
    36601 ttcagcttat cattgattat ggtttgactg atgtcgtttc tgaacgtttt gatgcaggcg
    36661 tccgtctggg tggggagatg gataaagata tgatcgccat tcgaatcggg ccagatatac
    36721 caatggctat tgttggctca ccggattatt tttctcgccg aagtgttcca acgtcagtgt
    36781 cacaattaat agatcatcag gcaattaatt tgtatcttcc cacatcgggt acagcaaatc
    36841 gctggagatt aatacgcggt ggacgtgaag ttcgtgttcg catggaaggt cagcttttac
    36901 tgaatacgat agacctgatc attgatgctg caattgatgg gcatggattg gcgtatctac
    36961 cttatgatca ggttgagcgg gcgattaaag aaaaaaaact gatacgtgtt ttggataaat
    37021 tcacaccaga tttacccggt tatcacctgt actatccaca ccgtcgacat gctggctcgg
    37081 cattctcatt atttatagat aggctgaagt ataaaggtgc tgtttagcac tacttgc
//
LOCUS       DBFIFS010000026        35001 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.026, whole genome shotgun sequence.
ACCESSION   DBFIFS010000026 DBFIFS010000000
VERSION     DBFIFS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.026"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..977
                     /locus_tag="N7A07_001173"
                     /pseudo
     CDS             1..977
                     /locus_tag="N7A07_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
     gene            complement(1127..2491)
                     /gene="mnmE"
                     /locus_tag="N7A07_001174"
     CDS             complement(1127..2491)
                     /gene="mnmE"
                     /locus_tag="N7A07_001174"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU2475874.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2597..4243)
                     /gene="yidC"
                     /locus_tag="N7A07_001175"
     CDS             complement(2597..4243)
                     /gene="yidC"
                     /locus_tag="N7A07_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU2475875.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(4246..4503)
                     /gene="yidD"
                     /locus_tag="N7A07_001176"
     CDS             complement(4246..4503)
                     /gene="yidD"
                     /locus_tag="N7A07_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU2475876.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4467..4826)
                     /gene="rnpA"
                     /locus_tag="N7A07_001177"
     CDS             complement(4467..4826)
                     /gene="rnpA"
                     /locus_tag="N7A07_001177"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU2475877.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4843..4983)
                     /gene="rpmH"
                     /locus_tag="N7A07_001178"
     CDS             complement(4843..4983)
                     /gene="rpmH"
                     /locus_tag="N7A07_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU2475878.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(5213..5293)
                     /locus_tag="N7A07_001179"
     CDS             complement(5213..5293)
                     /locus_tag="N7A07_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475879.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5590..6993
                     /gene="dnaA"
                     /locus_tag="N7A07_001180"
     CDS             5590..6993
                     /gene="dnaA"
                     /locus_tag="N7A07_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU2475880.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6998..8098
                     /gene="dnaN"
                     /locus_tag="N7A07_001181"
     CDS             6998..8098
                     /gene="dnaN"
                     /locus_tag="N7A07_001181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU2475881.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            8098..9171
                     /gene="recF"
                     /locus_tag="N7A07_001182"
     CDS             8098..9171
                     /gene="recF"
                     /locus_tag="N7A07_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU2475882.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            9200..11614
                     /gene="gyrB"
                     /locus_tag="N7A07_001183"
     CDS             9200..11614
                     /gene="gyrB"
                     /locus_tag="N7A07_001183"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU2475883.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11638..11736)
                     /locus_tag="N7A07_001184"
                     /pseudo
     CDS             complement(11638..11736)
                     /locus_tag="N7A07_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11854..12252
                     /gene="yidB"
                     /locus_tag="N7A07_001185"
     CDS             11854..12252
                     /gene="yidB"
                     /locus_tag="N7A07_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU2475884.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12367..13179
                     /gene="yidA"
                     /locus_tag="N7A07_001186"
     CDS             12367..13179
                     /gene="yidA"
                     /locus_tag="N7A07_001186"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU2475885.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(13225..13881)
                     /gene="yidX"
                     /locus_tag="N7A07_001187"
     CDS             complement(13225..13881)
                     /gene="yidX"
                     /locus_tag="N7A07_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU2475886.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            14017..14130
                     /locus_tag="N7A07_001188"
                     /pseudo
     CDS             14017..14130
                     /locus_tag="N7A07_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14127..15191)
                     /gene="cbrA"
                     /locus_tag="N7A07_001189"
     CDS             complement(14127..15191)
                     /gene="cbrA"
                     /locus_tag="N7A07_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU2475887.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            15292..16506
                     /gene="yidR"
                     /locus_tag="N7A07_001190"
     CDS             15292..16506
                     /gene="yidR"
                     /locus_tag="N7A07_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU2475888.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16508..16840)
                     /gene="yidQ"
                     /locus_tag="N7A07_001191"
     CDS             complement(16508..16840)
                     /gene="yidQ"
                     /locus_tag="N7A07_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU2475889.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    17080..17152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            17146..17559
                     /gene="ibpA"
                     /locus_tag="N7A07_001192"
     CDS             17146..17559
                     /gene="ibpA"
                     /locus_tag="N7A07_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU2475890.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17671..18099
                     /gene="ibpB"
                     /locus_tag="N7A07_001193"
     CDS             17671..18099
                     /gene="ibpB"
                     /locus_tag="N7A07_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU2475891.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            18295..19956
                     /gene="yidE"
                     /locus_tag="N7A07_001194"
     CDS             18295..19956
                     /gene="yidE"
                     /locus_tag="N7A07_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU2475892.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19953..20669)
                     /gene="yidP"
                     /locus_tag="N7A07_001195"
     CDS             complement(19953..20669)
                     /gene="yidP"
                     /locus_tag="N7A07_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2475893.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20965..22581
                     /locus_tag="N7A07_001196"
     CDS             20965..22581
                     /locus_tag="N7A07_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2475894.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22581..23903
                     /locus_tag="N7A07_001197"
     CDS             22581..23903
                     /locus_tag="N7A07_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGU2475895.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23900..24793)
                     /gene="yidL"
                     /locus_tag="N7A07_001198"
     CDS             complement(23900..24793)
                     /gene="yidL"
                     /locus_tag="N7A07_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2475896.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24960..26675
                     /gene="yidK"
                     /locus_tag="N7A07_001199"
     CDS             24960..26675
                     /gene="yidK"
                     /locus_tag="N7A07_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU2475897.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26672..28165
                     /gene="yidJ"
                     /locus_tag="N7A07_001200"
     CDS             26672..28165
                     /gene="yidJ"
                     /locus_tag="N7A07_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU2475898.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(28212..28661)
                     /gene="yidI"
                     /locus_tag="N7A07_001201"
     CDS             complement(28212..28661)
                     /gene="yidI"
                     /locus_tag="N7A07_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU2475899.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28770..29117
                     /gene="yidH"
                     /locus_tag="N7A07_001202"
     CDS             28770..29117
                     /gene="yidH"
                     /locus_tag="N7A07_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU2475900.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            29107..29469
                     /gene="yidG"
                     /locus_tag="N7A07_001203"
     CDS             29107..29469
                     /gene="yidG"
                     /locus_tag="N7A07_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU2475901.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29466..29963
                     /gene="yidF"
                     /locus_tag="N7A07_001204"
     CDS             29466..29963
                     /gene="yidF"
                     /locus_tag="N7A07_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU2475902.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29971..31155)
                     /gene="emrD"
                     /locus_tag="N7A07_001205"
     CDS             complement(29971..31155)
                     /gene="emrD"
                     /locus_tag="N7A07_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU2475903.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            31237..31311
                     /gene="ysdE"
                     /locus_tag="N7A07_001206"
     CDS             31237..31311
                     /gene="ysdE"
                     /locus_tag="N7A07_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU2475904.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31435..31524)
                     /gene="tisB"
                     /locus_tag="N7A07_001207"
     CDS             complement(31435..31524)
                     /gene="tisB"
                     /locus_tag="N7A07_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU2475905.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            32089..32187
                     /gene="ivbL"
                     /locus_tag="N7A07_001208"
     CDS             32089..32187
                     /gene="ivbL"
                     /locus_tag="N7A07_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU2475906.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            32293..33981
                     /gene="ilvB"
                     /locus_tag="N7A07_001209"
     CDS             32293..33981
                     /gene="ilvB"
                     /locus_tag="N7A07_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU2475907.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33985..34275
                     /gene="ilvN"
                     /locus_tag="N7A07_001210"
     CDS             33985..34275
                     /gene="ilvN"
                     /locus_tag="N7A07_001210"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU2475908.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34538..>35001
                     /locus_tag="N7A07_001211"
     CDS             34538..>35001
                     /locus_tag="N7A07_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475909.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVL"
BASE COUNT         8520 a         8794 c         9218 g         8469 t
ORIGIN      
        1 tcacaatact attgataagt ttaatgatta cataacaaat ttaaaagagt tgaccagaaa
       61 atataatttt agccaagagg acctggaaac tctactcctt gcgaaagata atctcggagt
      121 gagctggatt cccagggctt tgaaaaataa tcaaaataaa attgtcaaag catggttgtt
      181 ggcgatagat gactttgaga aagaatttgg ggtaaataaa aatgaaatac ttcttcgtat
      241 aggaaaggaa atagactcaa ttgatgattt aaatagcgct attagaacca atgattataa
      301 tgttgttaat atattgctag ccaatataaa agccaaaatg tttaaaaatg aattaaataa
      361 agaagatata ttgaaactga tggcagcaag agaaaaagtg gcgggagcat cagacaaatg
      421 gacgaaggca tcaggcttat attctgcgat agtgaaaggg catacgaaga ttgttgctgc
      481 ctggatggag acagctgaag tgatagccag ccattatgaa aatgataaag atgtagtgag
      541 agaactcctg tcgctgagca gaaataatgc agtttgctct ttgtatgttg ccagctataa
      601 gacaatgagt aagcaggtca ttgatgtata tctgaatgcg gcgattcgcc tggcgttgca
      661 acacgggttc actttcgatg agattttgga gcagtttacc cgtgactttg atgggaagtc
      721 attctctctt gcggtagaga aagcggatga tatatatggg tctctggctg aaaatattca
      781 aaattgtggt tggtgaaaat gaaaattatc ttaaagatgt gatgatgcaa ctggaagcga
      841 agaataatga aggcaaatat gtaataagca aggcaaatgg caatcctgta tttaaagaac
      901 tattctggaa agcaatagac gaatttaact ttccgcaaga ggagcttaat cggctaaagc
      961 aatatcgttc tctgtaaaga caggactgca agatgttgaa agtcgctcca gccagtagta
     1021 aaggcagttt tggtgcagga tgctcataaa aaaacaagcg ctgttccgtt ctgatagtta
     1081 gtttccccca gaacggaaca gtgcccttta tatcaattta gacgacttac ttaccaatac
     1141 agaagctgga gaaaatccgc cccagcaaat catcagaggt aaattccccg gtgatttcgc
     1201 ttaagttctg ctgtgccagg cgcaactctt ccgccagcag ttcgcccgcc caggcaccca
     1261 acagttgcgc tttaccttgc tgcaaatgct ccgctgcctg ttccagcgcc tgaaggtggc
     1321 gacgacgcgc caggaagccg ccttccatgt tggtgtcaaa gcccatgctc tgtttgagat
     1381 ggttacgcag cacgtccacg ccttcgccag tctttgccga gagacgaatc aacgcatgac
     1441 cgttcacttc actcattccc agcgtttcgc cagtgatatc ggctttattg cgtaccacgg
     1501 tgatcggcag tttcgcgggc agacgggcga taaattccgg ccagatctcc gccggatcaa
     1561 cggcgtctgt cgtggtgcca tcgaccataa acagcacgcg gtcggcttgt tcaatttctt
     1621 gccacgcgcg ctcgatacca atacgttcca cttcatcgct ggcttcacgt agcccggcgg
     1681 tgtcgatgat atgcagcggc attccgtcaa tgtggatatg ctcacgcaac acgtcacgcg
     1741 tggttccggc gatatcggtt acgattgctg cttcacggcc agccagcgcg tttaacaggc
     1801 tcgatttacc ggcattagga cgtccggcaa tcaccacctt catcccttcg cgcaacaaac
     1861 taccctgacg tgcttcagca cgcactgcat cgagatcggc aatcacgtca ttaagctggg
     1921 cttcaatttt tccgtcggag aggaaatcga tctcttcatc ggggaaatca attgccgctt
     1981 cgacgtaaat gcgcaagtgg gtgagggctt ccaccagatg gttgacccgt gcggagaatg
     2041 cgccttgcag cgagttaagt gccgaacggg ctgcctgttc cgaactggca tcgataagat
     2101 cggcaatcgc ctcggcctgg gctaagtcga gtttatcgtt aagaaacgcg cgttcggaaa
     2161 actcaccagg gcgagcaatc cgcaggccgg gaatggtcag aatgcgtttt aacagcaggt
     2221 cgagaatcac cggaccgcca tgaccctgca gttccagcac atcttcgccg gtgaacgagt
     2281 tcgggccagg gaaccatagc gcaatcccct gatcgagcac gctgccatcg gcgtctttaa
     2341 acggaagata atcggcgtag cgcggcttag gcagtttacc cagcacggtt tcggcaactt
     2401 cacgggcttt gaagccggag atgcgcagga tgccaacgcc gccacgtccc ggaggcgtgg
     2461 cctgggctac gatagtgtca ttatcgctca tgatgttcct gttgctttgt gtggcggata
     2521 cgcggtgctt atccgcccta cgaaaagaaa aaaggcggtc aactgaccgc ccttatttta
     2581 gcgaaaactc accgaatcag gattttttct tctcgcggct atgcaggcca cgtttttcca
     2641 gaccacggta aatcagctgc tgctgaataa tggttaccag gttgctgacg atatagtaca
     2701 gcaccagacc tgacgggaac cacaggaaga acacggtgaa gatgaccggc ataaaggtca
     2761 tgatcttctg ctgcatcggg tcggtcacag tggtcggcga catcttctga atgaagaaca
     2821 tcgttacgcc catcaggatc ggcaggatgt agtacgggtc ctgtgcagac aggtcgtgga
     2881 tccacagtgc aaacggtgcc tgacgcagtt caacggaacc catcagcatg tagtacaacg
     2941 ccaggaagat tggcatctgg atcagcagcg ggaagcagcc gcccagcggg ttaaccttct
     3001 cagctttgta cagcgccatc atttcctggc tgatacgctg tttgtcatcg cccagacgct
     3061 cacgcattgc ctgaatcttc ggctgcagca tacgcatctt cgccatggag gtgtactgcg
     3121 ctttggtcag cgggtacatg atgccacgaa cgataaaggt gatgatgata atggagaagc
     3181 cccagttacc cacaaagcta tggatccatt tcagcagttt gaacagcggc tgagagatga
     3241 accacaacca accgtaatca acggtcagat ccaggtgcgg agcaacagct gccattttgt
     3301 cctggatttc cgggccaacc cacagggtgc tgttcatcgc gccagtctga ccaggctgaa
     3361 ccagtaccgg ctgagattta tagccgatag cggcgatgcc gttacccaga ttagcagtat
     3421 agaagttgtt ggtaccgtcg ttatgcggga tccacgccgt cgcgaaatac tgttgcagca
     3481 ttgccaccca accacctttc gaagagatgt tcaggttttc gttatcggca atggtatcga
     3541 acttgtattt ctcgtacttc tcgtcaggcg tggagtacgc cgcgccacgg aaggtgtgca
     3601 gtgcgaagtt gctgcttccg gtatcgagat gcggtggcag agtgatggat tgcttcaact
     3661 gaccaaaggt ggagatttcc agcggtttct cgccagcgtt ctgcacgttg tagttgacgt
     3721 tgacagcgta atcaccccgt ttcaggacaa acgttttggt aaacgtgttg cctgccgcgt
     3781 cggtatacgt catcggcacc tgcagttcgt tttgaccttc agccagcaca taagcgtctt
     3841 tttcaacgtt atacagcgga cgcgggccgt tagccgggtt atctgggcca tcacgaccgg
     3901 tcagaccgct ctgtgcctga taaataaact gcggtgaagt ttccagcagc tggaacggct
     3961 gggtagagtt cagctctttc gggtaagcag gcagcagagc ttgctcaaca tcaccaccac
     4021 gggtgttgat ggtcagatca agcacgtcgg tcttaaccga gatcagtttc ccctggccac
     4081 tggccggtac gccctggtcg gcggcgctac ccgctgcggt ggtcgttgtc tgcgtggtct
     4141 gttgggcctg aggttgcggg tttttatcct gctcccaggc ttgccagatc atgaaagaca
     4201 cgaacagcaa agcgatgact aaaagattgc gttgcgaatc catcgttagt gttctctggt
     4261 attaaatggt ccgggcggga cgggatcgtc accaccaggg tgtaaagggt ggcattttaa
     4321 tacgcgtttc accgtcaacc aactgccttt tatcactcca aacctgcgca atgcctcaat
     4381 tccgtagctt gaacaggttg gagtgaaacg acaatgcggc ccgagtagcg gactaatcag
     4441 gcgttgatag acccgaatga gggctatcag gacccgcgag ccaggcgaca gtggcggcgc
     4501 cataattttt ccaacgcttc cgagagagca cggttatcga ggttggcaac cccttttttc
     4561 gccaccacca cgaaatccat agccgggagt tcatgttggc gcagacggaa gctttcacgc
     4621 gtcagacgtt taatccgatt gcgttcatgg gcgcgtcgaa cgtttttctt ggcgactgta
     4681 agaccgatac ggggatgccc cagcgaattc aggcggccga gaatggtaat ttgcggcgtg
     4741 ccagcccgtt gtggctgctg gaagacgaat gtgaattgac tgggagttaa caagcgtaac
     4801 tccctgggaa atgcgagctt aaccactcag gggttagctt tattacttag aaacggtcag
     4861 acgagcgcgg cctttagcac gacgacgtgc cagaacctga cgaccatttt tagtagccat
     4921 acgagcacgg aagccgtgag aacggttgcg cttcagtaca gacggttgaa aagtgcgttt
     4981 catggcgatt tctacctaaa cttgaataaa ttcaatggct ttattggata tccgccgaaa
     5041 aatgaaacga tggacaccga agccatgggt gattaaagag gccggattgt aataattgta
     5101 cactccggag tcaattctct ttccttattt accgcgcttt tccgcacctt ttcgcaggga
     5161 aaacgtacgg cctcacacca gtgaaaacca gcatggcgcg ccgggtggag gattatacgg
     5221 gctgatgggt aaagcgcaag gatcgtcctg gatctttatt agatcgatta agccaatttt
     5281 tgtctatggt cattaaattt tccaatatgc ggcgtaaatc gtgcccgcct cgcggcagga
     5341 tcgtttacac ttagcgagtt ctggatcatc ctgtggataa atcgggaaaa tctgtgagaa
     5401 acagaagatc tcttgcgcag tttaggctat gatccgcggt cccgatcgtt ttgcaggatc
     5461 ttgatcgggc atataaccgc agacagcggt tcgtgcgtca ccctcaagca gggtcttttc
     5521 gacgtacgtc aacaatcatg aatgtttcag ccttagtcat tatcgacttt tgttcgagtg
     5581 gagtccgccg tgtcactttc gctttggcag cagtgtcttg cccgattgca ggatgagtta
     5641 ccagccacag aattcagtat gtggatacgc ccattgcagg cggaactgag cgataacacg
     5701 ctggccctgt acgcgccaaa ccgttttgtc ctcgattggg tacgggacaa gtaccttaat
     5761 aatatcaatg gactgctaac cagtttctgc ggagcggatg ccccacagct gcgttttgaa
     5821 gtcggcacca aatcggtgac gcaaacgcca caagcggcag tgacgagcaa cgtcgcggcc
     5881 cctgcacagg tggcgcaaac gcagccgcaa cgtgctgcgc cttctacgcg ctcaggttgg
     5941 gataacgtcc cggccccggc agaaccgacc tatcgttcta acgtaaacgt caaacacacg
     6001 tttgataact tcgttgaagg taaatctaac caactggcgc gcgcggcggc tcgccaggtg
     6061 gcagataacc ctggcggtgc ctataacccg ttgttccttt atggcggcac gggtctgggt
     6121 aaaactcacc tgctgcatgc ggtgggtaac ggcattatgg cgcgcaagcc gaatgccaaa
     6181 gtggtttata tgcactccga gcgctttgtt caggacatgg ttaaagccct gcaaaacaac
     6241 gcgatcgaag agtttaaacg ctactaccgt tccgtagatg cactgctgat cgacgatatt
     6301 cagttttttg ctaataaaga acgatctcag gaagagtttt tccacacctt caacgccctg
     6361 ctggaaggta atcaacagat cattctcacc tcggatcgct atccgaaaga gatcaacggc
     6421 gttgaggatc gtttgaaatc ccgcttcggc tgggggctga ctgtggcgat cgaaccgcca
     6481 gagttagaaa cccgcgtggc gatcctgatg aaaaaggccg acgaaaatga cattcgtttg
     6541 ccaggcgaag tggcgttctt tatcgccaag cgtctacgat ctaacgtacg tgagctggaa
     6601 ggcgcgctga accgcgtcat tgccaacgcc aactttaccg gacgggcgat cactatcgac
     6661 ttcgtgcgtg aggcgctgcg cgacttgctg gcattgcagg aaaaactggt caccatcgac
     6721 aatattcaga agacggtggc ggagtactac aagatcaaag tcgcggatct cctttccaag
     6781 cgtcgatccc gctcggtggc tcgtccgcgt cagatggcga tggcgctggc gaaagagctg
     6841 actaaccaca gtctgccaga gattggcgat gcgtttggtg gtcgtgacca cactacggtg
     6901 cttcatgcct gccggaagat cgagcagctg cgtgaagaga gccacgatat caaagaagat
     6961 ttttcaaatt taatcagaac attgtcatcg taaacctatg aaatttaccg tagaacgtga
     7021 gcatttatta aaaccgctac aacaggtgag cggtccgtta ggtggtcgtc ctacgctacc
     7081 gattctcggt aatctgctgt tacaggttgc tgacggtacg ttgtcgctga ccggtactga
     7141 tctcgagatg gaaatggtgg cacgcgttgc gctggttcag ccacacgagc caggagcgac
     7201 gaccgttccg gcgcgcaaat tctttgatat ctgccgtggt ctgcctgaag gcgcggaaat
     7261 tgccgtgcag ctggaaggtg agcggatgct ggtgcgctcc gggcgtagcc gtttttcgct
     7321 gtctaccctg ccagcggcgg atttcccgaa cctcgatgac tggcagagtg aagtcgaatt
     7381 taccctgccg caggcgacga tgaagcgtct gattgaagcg acccagtttt ctatggcgca
     7441 tcaggacgtt cgctattact taaacggtat gctgtttgaa accgaaggtg aagaactgcg
     7501 caccgtagcg accgacggcc accgtctggc ggtctgttca atgccaattg gtcaatcttt
     7561 gccaagccat tcggtgatcg tgccgcgtaa aggcgtgatt gaactgatgc gtatgctcga
     7621 cggcggcgat aatccgctgc gtgtgcagat tggcagcaac aatattcgcg cccacgttgg
     7681 cgactttatc ttcacctcta aactggtgga tggtcgcttc ccggattacc gccgcgttct
     7741 gccgaagaat ccggacaaac atctggaagc aggctgcgat ctcctcaagc aggcgtttgc
     7801 tcgcgcggca attctctcta acgagaaatt ccgcggtgtg cgcctgtatg tcagcgaaaa
     7861 ccagctgaaa atcaccgcca acaacccgga acaggaagaa gcggaagaga tcctcgacgt
     7921 tacctatagc ggtgcggaga tggaaatcgg cttcaacgtc agctatgtgc tggatgttct
     7981 gaacgcgctg aaatgcgaaa acgtccgcat gatgctgacc gattcggttt ccagcgtgca
     8041 gattgaagat gcagccagcc agagcgcggc ttatgttgtc atgccaatga gactgtaatg
     8101 tccctcaccc gcttgttgat ccgcgatttc cgcaacattg aaaccgcgga tctcgcttta
     8161 tctcccggct ttaactttct ggtaggtgcc aacggcagtg gcaaaaccag cgtgctggaa
     8221 gccatctata cgctcggcca tggtcgggcg tttcgcagtt tgcagattgg tcgcgtcatt
     8281 cgccacgagc aggaggcgtt tgttctccac gggcgattac agggcgaaga gcgcgagacg
     8341 gcgattggct taaccaaaga caaacagggc gacagcaaag tccgcatcga cggtactgac
     8401 gggcataaag tcgcggaact ggcgcacctg atgccaatgc agctgataac gccagaaggg
     8461 tttactttac tcaacggcgg ccccaaatac agaagagcat tcctcgactg gggatgcttt
     8521 cacaacgaac ccggattttt caccgcctgg agcaatctca agcgattgct caagcagcgc
     8581 aatgcggcgc tgcgccaggt gacacgttac gaacagctac gcccgtggga taaagaactg
     8641 atcccgctgg cggagcaaat cagtacctgg cgcgcggagt atagcgccgg tatcgcggcc
     8701 gatatggctg atacctgtaa gcaatttctc cctgagtttt ctctgacttt ctctttccag
     8761 cgcggctggg agaaagagac agaatatgct gaggtgctgg aacgtaattt tgaacgcgat
     8821 cgccagctaa cctacaccgc gcatggcccg cataaagcgg acttacgcat tcgcgccgac
     8881 ggtgcgccgg tggaagatac cttatcgcgt gggcagctta agctgttgat gtgcgcctta
     8941 cgtctggcgc aaggagagtt cctcacccgt gaaagcgggc ggcggtgtct ctacctgata
     9001 gatgattttg cctctgagct tgatgatgag cgtcgcgggc tgcttgccag ccgcttaaaa
     9061 gctacgcaat cgcaggtctt tgtcagcgcg atcagtgctg aacacgttat agacatgtcg
     9121 gacgaaaatt cgaagatgtt taccgtggaa aagggtaaaa taacggatta acccaagtat
     9181 aaatgagcga gaaacgttga tgtcgaattc ttatgactcc tccagtatca aagtcctgaa
     9241 agggctggat gcggtgcgta agcgcccggg tatgtatatc ggcgacacgg atgacggcac
     9301 cggtctgcac cacatggtat tcgaggtggt agataacgct atcgacgaag cgctcgcggg
     9361 tcactgtaaa gaaattatcg tcaccattca cgccgataac tctgtctctg tacaggatga
     9421 cgggcgcggc attccgaccg gtattcaccc ggaagagggc gtatcggcgg cggaagtgat
     9481 catgaccgtt ctgcacgcag gcggtaaatt tgacgataac tcctataaag tgtccggcgg
     9541 tctgcacggc gttggtgttt cggtagtaaa cgccctgtcg caaaaactgg agctggttat
     9601 tcagcgcgag ggtaaaattc accgtcagat ctacgaacac ggtgtaccgc aggcaccgct
     9661 ggcggttacc ggcgagactg aaaaaaccgg caccatggtg cgtttctggc ccagcctcga
     9721 aaccttcacc aatgtgaccg agttcgaata tgaaattctg gcgaaacgtc tgcgtgagtt
     9781 gtcgttcctc aactccggcg tttccattcg tctgcgcgac aagcgtgacg gcaaagaaga
     9841 ccacttccac tatgaaggcg gcatcaaagc gttcgttgaa tatctgaaca agaacaaaac
     9901 gccgatccac ccaaatatct tctacttctc caccgaaaaa gacggtatcg gcgttgaagt
     9961 ggcgctgcag tggaacgatg gcttccagga aaacatctac tgctttacca acaacattcc
    10021 gcagcgtgac ggcggtactc acctggcagg cttccgtgcg gcgatgaccc gtaccctgaa
    10081 tgcctacatg gacaaagaag gctacagcaa aaaagccaaa gtcagcgcca ccggtgacga
    10141 tgcgcgtgaa ggcctgattg cggtcgtttc cgtaaaagtg ccggacccga aattctcctc
    10201 ccagaccaaa gacaaactgg tttcttctga ggtgaaatcg gcggttgaac agcagatgaa
    10261 cgaactgttg gcggaatacc tgctggaaaa cccaactgac gcgaaaatcg tggttggcaa
    10321 aattatcgat gctgcccgtg cccgtgaagc ggcgcgtcgc gcgcgtgaaa tgacccgccg
    10381 taaaggtgcg ctcgacttag cgggcctgcc gggcaaactg gcagactgcc aggaacgcga
    10441 tccggcgctt tccgaactgt accttgtgga aggggactcc gcgggcggct ctgcgaagca
    10501 ggggcgtaac cgcaagaacc aggcgattct gccgctgaag ggtaaaatcc tcaacgtcga
    10561 gaaagcgcgc ttcgataaga tgctctcttc tcaggaagtg gcgacgctta tcaccgcgct
    10621 tggttgtggt atcggtcgtg acgagtacaa cccggacaaa ctgcgttatc acagcatcat
    10681 catcatgacc gatgcggacg tcgacggctc gcacattcgt acgctgctgt tgaccttctt
    10741 ctatcgtcag atgccggaaa ttgttgaacg cggtcacgtc tacatcgctc agccgccgct
    10801 gtacaaagtg aagaaaggca agcaggaaca gtacattaaa gacgacgaag cgatggatca
    10861 gtaccagatc tctatcgcgc tggacggcgc aacgctgcac accaacgcca gcgcaccggc
    10921 attggctggc gaagcgttag agaaactggt gtctgagtac aacgcgacgc agaaaatgat
    10981 caaccgcatg gagcgtcgtt atccgaaagc aatgctgaaa gagcttatct atcagccgac
    11041 gctgacggaa gccgacctct ctgatgagca gaccgttacc cggtgggtga acgcgctggt
    11101 cagcgaactg aacgacaaag aacagcacgg cagccagtgg aagtttgatg tccacaccaa
    11161 tgccgaacaa aacctgttcg agccgattgt tcgcgtgcgt acccacggtg tggatactga
    11221 ctatccgctg gatcacgagt ttatcactgg tggcgaatat cgtcgtatct gcacgctggg
    11281 tgagaaactg cgtggcttgc tggaagaaga tgcgtttatc gaacgtggcg agcgtcgtca
    11341 gccggtagcc agcttcgagc aggcgctgga ctggctggtg aaagagtccc gtcgcggcct
    11401 ctccatccag cgttataaag gtctgggcga gatgaacccg gaacagctgt gggaaaccac
    11461 catggacccg gaaagccgtc gtatgctgcg cgttaccgtt aaggatgcga ttgccgctga
    11521 ccagttgttc accacgctga tgggcgacgc cgttgaaccg cgccgtgcgt ttattgaaga
    11581 gaacgccctg aaagcggcga atattgatat ttaatggcgt taacaatgcg agcgtgcctg
    11641 atgcgctacg cttatcaggc ctacgagact cctgcaatat attgaatttg catgtttttg
    11701 taggccggat aaggcgttca cgccgcatcc ggcaagaagc tataagaaaa gggcggagat
    11761 catctccgcc ctttttattt ctgcaatccg caattatcat cccgcagttc tatacttttt
    11821 gggtcaacac gtaacgcttt acaggtgatt atcatggggc tttttgatga agttgtcggc
    11881 gcctttctga aaggcgatgc ggggaaatat caggcgattt taagttgggt tgaggagcag
    11941 ggtggcattc aggtgctgct ggaaaaactg caaagtggcg gcttaggggc cattctctca
    12001 acctggctga gtaatcaaca gggcaatcaa ccagttagcg gcgagcaact ggaatcagca
    12061 ctcggcacaa acgcggtatc cgatcttgga caaaaacttg gcgtggatac cagtacggct
    12121 tcctgtttac tggcagaaca attgccgaag attattgatg cgctctcacc gcaaggtgaa
    12181 gtgtcaccac aagccaataa cgatctgctt tccgcaggca tggaactgct gaaagggaaa
    12241 ctcttccgct aagcaaatgg ggagcatgca acccgcgttg catccccctt ttccctcggg
    12301 tgttgtactg atttttgagc ggaatcgcgt tagcatgggt caggaaccaa tctacctggg
    12361 gaactcatgg ctatcaaact cattgctatc gatatggatg gcacccttct gctgcccgat
    12421 cacaccattt cacctgccgt taaaaatgcg attgccgcag ctcgcgcccg tggcgtgaat
    12481 gtcgtgctaa cgacgggtcg cccttatgca ggtgttcaca attacctgaa agagctgcat
    12541 atggaacagc cgggcgacta ctgcattact tataacggcg cgctggtaca gaaggccgct
    12601 gatggtaaca ccgtggcgca aactgctctc agctatgacg actatcgttt cctggaaaaa
    12661 ctctctcgcg aagtcggttc tcatttccac gccctggacc gcaccacgct gtacaccgcc
    12721 aaccgtgata tcagctacta cacggtgcat gaatccttcg ttgccaccat tccgctggtg
    12781 ttctgcgaag cggagaaaat ggaccccaat acccagttcc tgaaagtgat gatgattgat
    12841 gaacccgcca tcctcgacca ggctatcgcg cgtattccgc aggaagtgaa agagaaatat
    12901 accgtgctga aaagtgcgcc gtacttcctc gaaatccttg ataaacgcgt taacaaaggt
    12961 acgggtgtga aatcactggc cgacgtgtta ggtattaaac cggaagaaat catggcgatt
    13021 ggcgatcagg aaaacgacat cgcaatgatt gaatatgcag gcgtcggtgt ggcaatggat
    13081 aatgctattc cttcggtgaa agaagtggcg aactttgtca ccaaatccaa ccttgaagat
    13141 ggcgtggcgt ttgctattga gaagtatgtg ctgaattaat ctgtgggcgg gcaaacagca
    13201 taaacgcgtt tgcccgctta ctgattattg gtttactgca tatacaacca gaaattgcag
    13261 cggcgcagta atgatgtcca gcgtaacagt cactggcagt aatgctgcgg aaacaccatc
    13321 cggaatgcct ctgggtgaat agtattcata aaatcgcact ttaaatggat ggtagaacgc
    13381 catcaccttt gagtaatcca tgtttttatt tttctcatgg attgtgcctt ttaggttttc
    13441 cagtgacctg gtacagtgtt gaacaccaca ggcaaaacca taatgcgttg ccagtgcctt
    13501 ttcttcttcg ttattccagt agtagtaaag cgatattgtg ccggtaaatt tctttttccc
    13561 cgcatttaaa acaaatcttg cgtcattggc aacctgaata ttgtgccggt taagtgctgg
    13621 atcattgagc atcttaacga tgtcatcggc accatcagtg ataaggtaat ccagcgattc
    13681 ccctacaaaa acatagcctt ttgttccatt actatctttt gcctgcgata gcccagtgat
    13741 ggtgtctgac tgcgattgat actttcctga actacccttt gcggtatggg aaaccagagc
    13801 atacgagata cagcctgaaa gcatcagcgc cagtgggaat aaactggcag ccttaaaaaa
    13861 tcccttaaaa ttcaacttca tgattaatcc tttaaacgag tagctaagcc gttgaaagca
    13921 tactcttctt tccctcctgt ttagcgcctg atctcccttt tcagatttct gcccgatata
    13981 agaccttttc ttatgaggtg tttttttgtg gactaatatc tgttgcaagg ctatgggttc
    14041 gcacctgcac tggtttatat ctccgccgtc gcgttgattg gcgcgctctc ttacatcctg
    14101 ctggtgggcg atgtgacgcg cgttggctaa tctttcaact gtggaatatg cgccatacca
    14161 ctgcgcataa tccactttct taaagccggt gcggtcaggc atcggctttt tactatcttg
    14221 ccgaagagtt ttaaacgcag tttgcgggtg gcgtgccagt aggctgcgtt gatcttctct
    14281 ggctgcttca gtaacaccga acgcagaatc tctgcgctat ccagcgcata gcttatcccc
    14341 tccagcgagc tggcgctgat aaatcccgcc gcttcaccaa tcagaaaggc gttgtcctta
    14401 ccgcagacaa aatcctgcca gcgtgaggga aatagcaccg tgcatttttc gctttttacc
    14461 gccttaccaa actggaattg aaaggcgctc attttctctt tcagcgtcgt gaaacgcgtc
    14521 tgaccgtctt tcattggata ggcaccgcca aagataaaat aaccgtcttt gctgatactc
    14581 catgagtaac agtcagttat cgcattatca aagatgcatg agtagaacgg caccggatgt
    14641 ttctctgcga accactgctg gatagcgaca tatttacgaa tctgatggtc cgggtagaga
    14701 tatcgccgca ccatcgagtt ggcaccatct gcaccgacca gatagcgggc agtaatatgc
    14761 tgctcccaac cgtctgcacg aaaaatgaca tgccatttat catcctcacg ccagattttc
    14821 cggcacaggc tatcgtgata aacctcaacg ctggcgggga tcagcgattt catccacaag
    14881 tcgaaagcat ggcgattaat attgatatag cttcgctggt agttacgcgt gagcgatgcg
    14941 gcgacgtcga cagttttgac gctgaaaatc tgtggattgg cgatcacatc gacaggaagc
    15001 gtcagtccat cgcgaataaa agatcgctgc gcgtccggtg ccagcagacc gccacaaggc
    15061 ttgctgaaac cttcagtacc atgctggtgc tttttatcca gcgcgatcac ctgcattttg
    15121 ccggctaact ttcgtgccaa cgccgaccct gccgggccga ggccaataat cgccacgtcg
    15181 aaatgttcca tcttttcact cacattcatc acgatggcaa aagactattg tctgaaagtg
    15241 aagtccttgt gaagtatgat ggcgggaacc gttttgtatc aggaccgcgc catgaaacag
    15301 ataacctttg ctccccgtaa tcacctgctc accaatacca atacctggac gcccgacagc
    15361 cagtggctgg tatttgacgt gcgtccttct ggcgcgtcgt ttaccggcga gaccattgag
    15421 cgtgtgaata tccataccgg cgaggtcgag gttatctatc gcgtgtcaca gggcgcgcac
    15481 gtcggcgtgg tgaccgttca tccaaagtca gagaaatatg tctttatcca cggacctgaa
    15541 aatcctcatg aaacatggca ttacgatttc catcaccgtc gcggcgtgat tgttgaaggc
    15601 ggcaagatga acaatctcga tgcaatggat attaccgttc cgtacacatc aggggcgctg
    15661 cgtggcggca gccatgtgca tgtctttagc ccgaacggtg aaatggtgag ttttacctat
    15721 aacgaccatg taatgcatca gttcgattcg gcgctggatt tgcgaaacgt cggggttgct
    15781 gcaccgtttg gcccggtcaa cgtacaaaag cagcatccgc gtgaatacag cggtagccac
    15841 tggtgcgtgc tggtgagtaa aaccacgccc acgccgcagc ctggcagcga tgaaatcaat
    15901 cgtgcttatg aagaaggatg ggtaggaaat cacgcgctgg cgtttattgg cgacacactt
    15961 tcgccaaagg gcgagaaagt gccggagctg tttatcgttg agttaccgca agatgaagct
    16021 ggctggaaag cagcaggtga tgcgccgtta agtggaacgg aaacaaccct gcccgcgcca
    16081 ccgcgtggca tcgtgcagcg acgtttaacc tttacccacc atcgggctta tccggggtta
    16141 gtcaacgtcc cgcgccactg ggtgcgctgt aatccgcagg gtacgcaaat cgcgttttta
    16201 atgcgagatg ataacggcat tgtgcaactg tggcttatct cgccacaggg cggcgagccg
    16261 cgccagttaa cccataacaa aacggatatt cagtctgcat ttaactggca tccgtcagga
    16321 gaatggttgg gctttgtgct gggtaatcga attgcttgtg cccatgcgca aagcggcgag
    16381 gttgagtatt taaccgaaaa ccacgccaat ccaccttctg cggacgccgt ggtcttctcg
    16441 ccggatggtc agtggctggc gtggatggaa ggcggccagc tgtggatcac cgaaactgat
    16501 cgctaaatca gttatcaggc attctggccg gtggaataac cgcattggtg gcttgcgcgt
    16561 tggcttcgct tttttcaacg cgtgagcgca ccgagctgtc tttgcggaac acatcccacg
    16621 gtaataaaag cgtgtccatc acagcggtaa acggcatatc gagaatcgcc agcgacttag
    16681 tgccccagtt ggtttcatca tcgccaatca tggtggcgct ggcgcgggtg ccgggatacg
    16741 ttccttcttt ccctccggta tgggacatca cgctggagca tcctccgagt aatgccattc
    16801 cgctgcatat catgaacgct aacaacacat ttcttatcat aataaattca tctgttgatc
    16861 gtgggtgttg gcctgatgag ttatagcgat cccttgctga aaataacatc atcattacgt
    16921 cgctctgtgg cggctatcgc actttaacgt ttcgtgctgc cccctcagtc tatgcaatag
    16981 accataaact gcaaaaaaaa gtccgctgat aaggcttgaa aagttcattt ccagacccat
    17041 ttttacatcg tagccgatga ggacgcgcct gatgggtgtt ctggctacct gacctgtcca
    17101 ttgtggaagg tcttacattc tcgctgattt caggagctat tgattatgcg taactttgat
    17161 ttatccccgc tttaccgttc tgctattgga tttgaccgtt tgtttaacca cttagaaaac
    17221 aaccagagcc agagtaatgg cggctaccct ccgtataacg ttgaactggt agacgaaaac
    17281 cattaccgca ttgctatcgc tgtggctggt tttgctgaga gcgaactgga aattaccgcc
    17341 caggataatc tgctggtggt gaaaggtgct cacgccgacg aacaaaaaga gcgcacctat
    17401 ctgtaccagg gcatcgctga acgcaacttt gaacgcaaat tccagttagc tgagaacatt
    17461 catgttcgtg gtgctaacct agtaaatggt ttgctgtata tcgatctcga acgcgtgatt
    17521 ccggaagcga aaaaaccgcg ccgtatcgaa atcaactaat ttcctaaggc cgccttgcgc
    17581 ggcctgacat ctccatgctc gccgtcaggg agcatatgcg aatcttcgga tttgcaggta
    17641 cttactcgct tcttagaagg agaaatgact atgcgtaact tcgatttatc cccactgatg
    17701 cgtcaatgga tcggttttga caaactggcc aacgcactgc aaaacgccgg tgaaagtcag
    17761 agcttcccgc cgtacaacat tgagaaaagc gacgataacc actaccgcat tacccttgcg
    17821 ctggcaggtt tccgtcagga agatttagag attcaactgg aaggtacgcg cctgagcgta
    17881 aaaggcacgc cggagcagcc aaaagaagag aaaaaatggc tgcatcaagg gcttatgaat
    17941 cagccattta gcctgagctt tacgctggct gaaaatatgg aagtctctgg cgcaaccttc
    18001 gtaaacggtt tactgcatat tgatttaatt cgtaatgagc ctgaacccat cgcagcgcag
    18061 cgtatcgcta tcagcgaacg tcccgcgtta aatagctaac tagctattct ctttgccccg
    18121 ccattctggt ggggcttttt ttgtctttct tcttcaacaa tgcgttaaat ttcgactgtt
    18181 taagatattt cggcacgttc tttcccgtga taatgtgcgc tataaggcaa atctgcttca
    18241 ccgattcagc cataatccgt agcaattaaa atgttatctc aagagaaatg gacgatgagt
    18301 gatatagcat taacggtcag tattttggct ttggtggcag tcgtcggttt gtttatcggc
    18361 aacgtcaaat ttcgcggcat aggattaggt attggcggcg tgctgtttgg tgggatcatc
    18421 gtcggccatt ttgtttctca ggcggggatg acattaagta gcgatatgct gcatgttatt
    18481 caggaatttg gcctgatcct gttcgtttat accatcggga ttcaggtagg gccgggcttc
    18541 tttgcctcat tgcgcgtctc cggattacgc ctcaacctgt ttgctgttct gatcgtcatc
    18601 atcggtggtc tggttaccgc catcctgcat aaactgtttg atattccact gccggtagtg
    18661 ctggggattt tctccggtgc ggtaaccaat acgccagcgc tgggggcagg gcagcagatt
    18721 ttgcgcgacc tgggtacacc aatggaaatg gtcgatcaga tggggatgag ttacgcgatg
    18781 gcgtatccat tcggcatttg cgggatattg ttcaccatgt ggatgttgcg ggttattttc
    18841 cgcgtcaatg tcgagacaga agcccagcag cacgagtctt cacgcaccaa tggcggcgcg
    18901 ctgatcaaga ctatcaatat tcgcgttgag aaccctaacc tgcatgattt agccattaaa
    18961 gatgtaccga ttctcaacgg cgacaaaatt atctgctcgc gtctgaaacg cgaagaaacc
    19021 ctaaaagttc cttcgccaga taccattatc caactgggcg atttgctgca tctggtgggt
    19081 cagccagcgg atttacataa tgcgcaactg gtgattggtc aggaggtcga tacttcgctg
    19141 tccacgaaag gcactgattt gcgcgtcgag cgtgtggtgg tcaccaatga aaacgtgctt
    19201 ggtaagcgta ttcgcgacct gcattttaaa gaacgctatg acgttgttat ctcgcgcctg
    19261 aaccgtgccg gggtcgaact ggtcgccagt ggcgatatca gcctgcagtt cggcgatatc
    19321 ctcaacctgg tggggcgtcc gtccgcaatt gatgccgttg ccaatgtgct ggggaatgcg
    19381 cagcaaaaac tgcaacaggt tcagatgctg ccagtgttta ttggcatcgg gcttggcgta
    19441 ttgttaggtt ctattcccgt ctttgtgcca ggattcccgg ccgcgttgaa actggggctg
    19501 gcgggcggtc cgctgattat ggcgttgatc ctcgggcgta tcggcagtat cggcaagctg
    19561 tactggttta tgccgccaag tgccaacctc gcgctgcggg aactggggat cgtgctgttc
    19621 ctctcggtcg ttggtctgaa atctggtggg gattttgtga ataccctggt caatggcgaa
    19681 gggctaagct ggattggtta tggtgccctg atcaccgccg ttccgctgat tactgttggc
    19741 attctggcgc ggatgttagc caaaatgaat tacctgacca tgtgcgggat gctggcaggt
    19801 tccatgaccg atcctccggc gctggcgttt gctaataatc ttcatccaac cagcggtgcg
    19861 gcggcgctct cttacgccac tgtctatccg ttagtgatgt tcctgcgcat tatcaccccc
    19921 caattactgg cggtgctctt ctggagtatc ggttaactct tgtccgggtg gagtcgccgt
    19981 aaatggtact ccacctggta ttcgctggca tttctgaaca tcactgaata attcaaaaac
    20041 tccccgctct cgctatatga caacgaggtg atccgcagca gtggcgtgtg ttccgctacc
    20101 ttcatttgcc gcgccagcag tctatcggcg agcaccggcg tcaggctttc ataattaccg
    20161 ccaatcaaaa tcccgcactc ttgttcaata tattcaaact tcgacccttc cagatgttgc
    20221 agcgaaaggt tacggaacag ttttaccggc atatagctgt cttccagcat cagcggtttc
    20281 ccttccacaa aacgaacgcg acgggagaag tagatctgct cgttgatttg aatccgcaac
    20341 tggctggcaa ttgccggagg cgcaggcatg atttcaaaaa tcagcacctg gctggtgact
    20401 gtttttccct gccgttttaa cacctccacc agcccggtca ggctggcggt ttgatgcagc
    20461 acatctttgc gcaccaggta agtgccgctg ccgtggcggc ggaccaccag cccccacgct
    20521 accagcaggt caatcgcttt acggatggtc atccgcgata ccgcaaactc ttccgccagc
    20581 tttttttcac cgggaagaag gctgtttagc gtgaaatccg cggaattaag tcgaattctt
    20641 aaccgctccg caatgctttt gtagatcatc tctagatctc ttttttgcct gcaagcccgg
    20701 gtaatgcgcc cacaagttct gcttaatcaa ttgaaaaaac atcttttttt taaagatgtg
    20761 ttcgatgctg tgtaccagtg ctcacagaga tctacttttt cgcgaaaaag tagatctcta
    20821 ccgcccaacg aaaagcatga aagcgatcac gaatcccatt gggtcgtcat gttctgccga
    20881 tcgcatcttc ctatcctcgc tccaggcctg ccgcataacc aatcaggctt cctacttaca
    20941 gaattgagaa aagaggatgt ggaaatgctc agtcaaattc aacgctttgg cggcgcgatg
    21001 ttcacgccag tgctgctgtt tcccttcgcc gggattgtgg tgggtcttgc catcttgctg
    21061 caaaacccga tgtttgtcgg ggaatcactg accgatccga acagtttatt cgcgcaaatc
    21121 gtacacatta ttgaagaggg cggttggacg gtattccgta atatgccgct gatttttgct
    21181 gtcggtttac ccattggcct tgctaagcaa gcgcaggggc gtgcttgtct ggcggtgatg
    21241 gtgagtttcc tgacctggaa ctatttcatc aatgcgatgg gaatgacctg gggaagctac
    21301 ttcggcgtcg atttcactca ggacgcggtg gcaggtagcg gtctgacaat gatggccggg
    21361 attaaaaccc tcgataccag cattatcggc gcaattatca tttccggcat tgtgacggcg
    21421 ctgcataacc gtctgttcga taaaaaactg ccggtttttc tcggcatttt ccaggggacg
    21481 tcttatgtgg tgattatcgc cttcctggtg atgatcccct gtgcctggct gacattgctc
    21541 ggctggccaa aagtacaaat ggggattgaa tctctgcaag cgttcctgcg ttcggcgggt
    21601 gcgcttgggg tgtgggttta caccttcctc gaacgtattc tgatcccaac cggtttacac
    21661 cacttcatct acggaccgtt tatctttggt ccggcagctg ttgaaggcgg aattcagatg
    21721 tactgggcgc agcatctgca agagttcagt ttgagcgccg agccgctgaa atcgttgttc
    21781 ccggaaggag gttttgccct gcacggtaac tcaaaaatct ttggtgccgt gggcatttct
    21841 ttagcgatgt acttcactgc cgcaccggaa aatcgggtaa aagtggcggg tttgctgatt
    21901 cccgcaacct taaccgccat gctggtggga attaccgaac cgctggaatt taccttcctg
    21961 ttcatttcac cgttgctgtt tgcggtacac gctgtgcttg cggcctcaat gtcgaccgtg
    22021 atgtatctct ttggtgtggt gggcaacatg ggcggaggtc tgattgacca ggttttaccg
    22081 caaaactgga tcccgatgtt cagcaaccac gcggatatga tgctgaccca aatcgccatt
    22141 gggttgtgct ttaccctgct gtacttcgtg gttttccgca ccctgattct gcaattcaac
    22201 atgtgcacgc cgggacgtga agatgcggaa gtgaaactct actcaaaagc cgaatacaaa
    22261 gcctcgcgag gccaaaccac cgcggcagag ccaaaaaaag agctggatca ggctgccggt
    22321 atcctgcaag ccctgggcgg ggtcggcaat atctccagca ttaacaattg tgcgacgcgt
    22381 ctacgcattg cactgcatga catgtcacaa acgctggatg acgaagtctt taaaaagctg
    22441 ggagcgcacg gcgtcttccg tagtggcgat gccattcagg tgatcattgg tctgcatgta
    22501 tcccagctgc gtgaacagct cgatagctta attaattctc atcaatcagc agaaaatgtt
    22561 gccattacgg aggcagtata atgaccaaat tctcagtggt tgtcgcaggc ggtggaagca
    22621 cctttacgcc aggcatcgtg ttgatgctcc tggcgaatca ggaccgtttc ccgcttcgtg
    22681 cactgaaatt ttatgataac gatggttcgc ggcaggaagt gattgccgaa gcctgtaaag
    22741 tcatccttaa agaaaaagcg ccggatattg cgtttagtta caccaccgat cctgaagtgg
    22801 cgttcagcga cgttgatttt gtcatggcgc acatccgcgt cggcaaatac ccgatgcgcg
    22861 aactggatga aaaaatcccg ctgcgccacg gcgttgttgg tcaggaaact tgcggacccg
    22921 gcggaatagc gtacggcatg cgttccattg gcggcgtcct ggaactggtg gattatatgg
    22981 aaaaatattc accaaacgcc tggatgctca actactccaa cccggcagca attgttgcag
    23041 aagccacgcg tcgtctgcgc ccgaatgcga aaatcctcaa catctgtgac atgccaatcg
    23101 gtattgaaag ccggatggcg caaattgttg ggctgcaaga tcgcaaacag atgcgcgtgc
    23161 gctactacgg cctgaaccac tttggctggt ggacatcaat tgaagatttg cagggcaacg
    23221 acctgatgca ccagctgcgg caatatgtct ctaagcacgg ttatgttcca ccgcagcaag
    23281 atacacatac tgaagcgagc tggaacgaca cctatgcaaa agcgcgggat gtccaggcac
    23341 tggacccgga tacattgccg aacacctatc tgaaatatta tctcttcccg gattatgtcg
    23401 ttcagcattc caaccctgaa catactcgcg cgaatgaggt aatggaacat cgcgagaaac
    23461 aggttttcga tgcttgccgc gccattacgg cggcaggaaa ttcagcggcg ggcaagctgg
    23521 aaattgacga acatgcgtca tacatcgtcg atctggcggc ggcaattgcc ttcaacactc
    23581 aggagcggat gttgctgatt gtgcctaaca acggggcaat ccataacttt gatgatgaag
    23641 ctatggtcga gatcccgtgt ctggtcgggc acaacggacc agaaccactg gtggtcggcg
    23701 atatcccgca gtttcagaaa gggttaatga gtcagcaagt ggcggtggaa aaactggtcg
    23761 tggacgcctg ggaacagcgt tcatatcagc acctgtggca ggcgattacg ttgtcgaaaa
    23821 cggtaccgag cgcctcggtc gccaaagcta ttctggatga gttgctggag gccaacaaag
    23881 cgtactggcc agagttacgt taattgtgtg gactggcatc cgttgggtgc tggtccgtat
    23941 tctttagcac ggctgaaggc gcataaccaa atttatgttt aaaggctttg ctgaaatgaa
    24001 aggagtcgaa gaagttgagc gtatcagcga cttcgccaac cgagtttcct tgttgcttta
    24061 atagcgatag cgccagatcc agacgcgcat cgaggtaata ttctttcggc gtctttccgg
    24121 tatagcgtaa aaacagacga cgcaaccagg cttcgctaca ggggatcgtg gcagccatat
    24181 cggctacgct ccagcgttgt tgcagactgg catgtaaagt ggcaattaat ttttcaattt
    24241 gccgccgttg tggatctttt ttaccgtctg cgtacataag acaaatccac tgataaataa
    24301 ttttcgtcag aaaagcgact gccagattat tttttattgc ttctggtgaa gttattaact
    24361 cagcaacttc ggtgagttcc tgattataaa tttcgccatt gtaaataacg ctttgctgac
    24421 cgatgggaat atccatcata ctggtggggg taaattccat ccagtattgt tcccagacta
    24481 aaccttcaca gtgataagag tgaatatcca ttggctttaa aaatataata cagttgccat
    24541 gcagtgttat ttgttcgcca ttctttaaaa gaatcttccc acatccttgt acggtataca
    24601 ccgcaaccca tgagttggcg ataagcggtt tcttcttgtc ccgtggccag atcacccggt
    24661 aatgctcgtc ggcgtaggta tgccagagcg agatcagaga aattccgctg gagatcacat
    24721 tttcgttaat caataaagga atattgtatg gagtttcgtt ttttacatct gaagtttgca
    24781 attttccatt cattttaata tccctccggc gaattatgct cacaacctga acttaacaga
    24841 aaacaatccg ttcagggata cgacgtttgt attttaagaa tctgactgcc tgacccgacg
    24901 cattttacct ctccctatat tcatgcgtcc gggacataaa cataaataag ggctatgaga
    24961 tgaattcgtt acaaatcttg agttttgtcg gttttacgct gctggtggcg atcatcacct
    25021 ggtggaaggt tcgcaaaaca gataccggat cgcaacaagg ctattttctt gccggacgtt
    25081 cactaaaagc gccggttatt gccgcttcgt taatgttaac caacctttcc acggaacaac
    25141 tggttggtct ttccgggcag gcctacaaaa gcggcatgtc ggtgatgggc tgggaagtga
    25201 cttcagcggt gacgctgatc ttcctcgcgc taatcttttt accgcgctat ctgaagcgcg
    25261 gcattgccac catccccgat tttctggagg aacgttatga taaaacgacg cgtatgatca
    25321 tcgacttctg cttcctcatt gccaccggcg tctgctttct gccgattgtt ctctactccg
    25381 gcgcgttggc gctcaacagc ctgtttcacg tcggggaatc gctacagatt tctcacggtg
    25441 cggctatctg gctactggta attttgcttg gtctggcggg aattttgtat gcggtgatcg
    25501 gcggactgcg cgcaatggca gtggcggact ccatcaacgg tattgggctg gttatcggcg
    25561 ggttgatggt gccggtattt ggcttgatcg cgatgggcaa gggcagcttt atgcagggca
    25621 ttgagcaaat taccaccgtt cacgccgaga aattaaactc agtcggtggc ccgaccgatc
    25681 ccttgccgat tggcgcggca tttaccggtt taattctggt gaacaccttt tactggtgta
    25741 caaatcaggg catcgtgcaa cgcacgctgg cgtcaaaaag cctggcggaa gggcaaaagg
    25801 gggcgctgtt aacggcggtg ctgaaaatgc tcgacccgct ggtactggtg ctgccagggt
    25861 tgattgcgtt tcatctgtat caggatctac cgaaagccga catggcctac ccgacgctgg
    25921 tcaataacgt tctgccagtg ccactggtgg gtttcttcgg cgcggtgtta tttggtgcag
    25981 tgatcagtac cttcaacggc tttctgaata gcgccagtac gttattcagt atgggtattt
    26041 accgtcgcat cattaaccag aatgccgagc cgcagcagct ggtcaccgtc gggcgcaaat
    26101 ttggtttctt tatcgctatc gtttcggtgc tggtagcgcc gtggatcgcc aacgcgccgc
    26161 aggggctgta tagctggatg aaacagctca acggcattta caacgtgccg ctggttacca
    26221 tcatcattat gggctttttc ttcctgcgca tcccggcgct ggcggcaaaa gtggcgatgg
    26281 ggattggcat aatcagctac atcaccatca actatctggt gaagttcgac ttccatttcc
    26341 tctatgtgct ggcctgtacg ttctgcatca acgtggtcgt gatgctggtg atcggtttta
    26401 tcaaaccgcg cgccacgccg ttcaccttca aagatgcgtt tgcggtggac atgaaaccgt
    26461 ggaaaaacgt caagatcgcg tcaattggca tcctgttcgc gatgattggc gtctatgccg
    26521 ggctggctga attcggcggc tacggtacgc gctggttagc gatgatcagt tatttcattg
    26581 ccgccgtagt gattgtctac ctgatttttg acagctggcg gcatcgtcac gacccagccg
    26641 taacctttac tcccgacgcg aaggatagcc tatgaaacgc cccaattttc tgttcatcat
    26701 gaccgatacc caggccccca atatggtcgg ttgctatagc ggtaaaccgc tgaatacgca
    26761 aaatattgat agtctggcgg cggaaggtat tcgctttaat tccgcctaca cctgttcacc
    26821 ggtttgtaca ccggcgcgcg ccgggctgtt taccggtatc tacgctaacc agtccgggcc
    26881 gtggaccaac aacgtcgcgc cgggtaaaaa catctccact atggggcgct actttaagga
    26941 tgccggctat cacacctgtt acatcggcaa atggcatctc gacggtcatg actatttcgg
    27001 cactggcgag tgtccgccgg agtgggacgc tgattactgg ttcgatgggg cgaactatct
    27061 tagcgaactg acggaaaaag agattagcct gtggcgcaat ggcctaaaca gcgtcgaaga
    27121 tttacaggcg aaccatattg acgaaacctt cacctgggcg caccgcatca gcaatcgagc
    27181 ggtggatttt ctgcaacagc ccgcgcgcgc cgaggaaccc ttcctgatgg tggtttcgta
    27241 tgatgagccg catcacccgt tcacctgtcc ggtggagtat ttagagaaat acgctgattt
    27301 ttactacgag ctgggcgaga aagcacagga tgacctggcg aacaaaccag aacatcaccg
    27361 cttatgggcg caggcgatgc catcgccagt cggtgatgac gggctttatc accatccgct
    27421 ctattttgcc tgtaatgact ttgttgatga ccaaatcgga cgggtcatca atgccttaaa
    27481 gccagagcaa cgtgaaaata cgtgggttat ttatacctcc gatcacggcg aaatgatggg
    27541 cgcacataag ctgatcagta aaggggcggc gatgtatgac gacatcaccc gcattccgct
    27601 gatcatccgt tcgccgcaag gggagcggcg acaggtcgat acgccagtca gtcatatcga
    27661 tttactgccg acaatgatgg cgctggcaga tattgaaaaa ccagagattc tgccggggga
    27721 aaatatcctt gcagtgaaag agccacgcgg cgtgatggtg gaatttaacc gctacgagat
    27781 tgagcatgac agctttggcg gttttattcc ggtgcgttgc tgggtgacgg atgactttaa
    27841 actggtgctc aacctcttca ccagtgatga actttacgat cgccgtaatg atccaaatga
    27901 aatgcataac ctgatcgatg atgcccattt tgccgacgtt cgcagcaaaa tgcatgatgc
    27961 cttattggat tatatggaca aaattcgcga tccgttccgc agttaccaat ggagcctgcg
    28021 tccgtggcgt aaagatgcac agccgcgctg gatgggggca tttcgtccac gcccacaaga
    28081 tggttattcg ccagtggtac gcgactatga caccggcctg ccgacacaag gggtgaaggt
    28141 ggaagagaaa aaacagaagt tctgacgata atgcccgctg atgaacaccc ggcgggcatt
    28201 attcaggctg cttaaaccag tgaaccgcca gtcaggaagg aaaagataag gaatatcagc
    28261 aaaatgctgc acacgatgcc gataccaaaa agcaaggtat ggaaagccag cgtcccgccg
    28321 ccaaaaaccc gcgcgccgcg aatgccccag cgattttcct tacgcatacc gccaataaat
    28381 gcgcaaagta gcgcagcaat ggccagtgcg ataccgcttt gatcgaggat tttatcgaca
    28441 tccacgtcct cttttctctc cacggtggta attttttcgc cttttattcc ggcgagaaga
    28501 cctcttttaa tttcagccgt tttgtccgcc acgatgcttt caagcgttgg cggtggtgcg
    28561 gaaaaagggc cgaatgaaaa gtggataatc cccatcatta aagcgatggc accgaatagc
    28621 atgcccaatg aactgatttt attttgcgct atgataccca tctgacctcc ttgtagcgga
    28681 tggcaaaagc atatcaggaa acgggcggac gaaattctta aaaaaaaccg ctaaaggtag
    28741 agcgttagtg aaattgcaaa ggagcaacaa tgaaaatttc ccgcctcgga gaagcgcctg
    28801 attaccgctt ctcgctggct aatgagcgta cttttctggc gtggatccgt actgcactag
    28861 gatttctggc ggcgggggtt gggcttgatc aacttgcgcc agatttcgcc acgcccgtca
    28921 ttcgcgaact gctggcgttg ttgttgtgtc tgttttccgg cgggttagcg atgtatggct
    28981 acttgcgctg gttacgcaat gaaaaagcga tgcgtctgaa agaggacctg ccctacacca
    29041 acagcttatt aatcatcagc ttaatattga tggtcgtagc agtgatcgtt atgggactgg
    29101 tgttgtatgc cggatagccg caaagccaga cgcattgccg acccagggct gcagccggag
    29161 cgtacatcac tggcgtggtt tcgtaccatg ctgggctacg gcgcgttgat ggcgttggct
    29221 atcaaacaca actggcacca ggcgggcatg ttattctgga tttcgattgg catcctcgcc
    29281 atcgtggcgc tgatcctctg gcactacact cgtaatcgca atttaatgga tgtcacgaat
    29341 agcgattttt cccaatttca cgtagtccgt gacaaatttt tgatctccct cgcggtgtta
    29401 tctctcgcaa tactgtttgc tgtaacgcat atacatcaac ttatcgtatt tattgagaga
    29461 gtcgcatgac aggaagtcag gtcattgatg ctgaagagga caggcataaa ctggttgtag
    29521 agtacaagga tgctctgcaa cctgccgatt tttatcacaa tttcaaacaa cggggcatcc
    29581 gctccgtaca gcttattccc catcttgaat ttgatgaccg gggcgatatg acggccgctt
    29641 cggtgacagc agagctgtgg gggaaatttt taattgccct gtttgagtgt tgggtgcggg
    29701 cagatatcag tcgtatctcg attgagcttt ttgacgccac attacaaaaa tggtgtggca
    29761 gcgaaaaccc gcaaccgcgc cgtggctgcc aggcgtgcga ctggcatctg ctgtgtccgc
    29821 acgctcggga ggagacgcca gacagcgtgc tttgtgcggg ctatcaggcg ttttattctt
    29881 actctgcgcc gcatatgcgg gtgatgcgtg atctgataaa gcagcatcgt tctcccatgg
    29941 agctgatgac gatgctgcgg tgacgtgagc ttaaacgggc tgcccctgat gcgacatccg
    30001 cgtcgccagc ggtagccagc acagcacgat caacaatccc attaaggtca tcaacaaccc
    30061 caggctgccc tgaccggttt gcggcaacat cgcagagagc gacgccagca cgccggaacc
    30121 aatgttttgt agaccgccga ccagcgcgcc agccgtgccc gccaggaaag ggaacggctc
    30181 catcgcgccg ctggtcgcca gcggaaacag catcccggca ccgaaaaaga acagcgcggc
    30241 gggaacgagc agcgtccaga cattcatcac gccaaaccag tcggggatcc acatcagtaa
    30301 gccagccagc aggcagcaga taaccgactg ccacatcaac gttgagaagc gtttattagg
    30361 acgtccggca aaccatgccc caaaaaatgc cgccggaatc ggcagaataa acaaaatact
    30421 gaccgtcata ctgctcagcc ctaacaccgc gcccatcagc acgccggagc aggcttcaaa
    30481 ggcggcaatc ccggccagac cgccaatcag catcagcaaa taacagttaa aaccgctgtt
    30541 accgaaaagc gttttataac tggtaagcag gcgcgtgcgc ggtgcgtcga ccggacgcgt
    30601 ttccggcatc cagcgggcca tactgaaggt cacaccggca caaagtacca acaaaaagag
    30661 ataacaggcg cgccagttcc acatcgtatc cagcagaccg ccgattagcg gtgcgagcaa
    30721 cggactgaca agaattccca tgtttaacag gctgttagcg tggcgcaact gtgtccgttc
    30781 atataaatca cgcggcaaag tacgcgccat tacgccgcca acgccggtgc ccatcccctg
    30841 catcgcgctg gcggcaatca atactgtcaa actggaggtc gtgaccgcga ccagcgttgc
    30901 cagcataaaa atggacattc cgacgaggat caccggtcgg cgacccacac ggtcggaaat
    30961 cgggccataa aacagctgtg agacaccgta agtcagcaga taagcgccca ttacgctctg
    31021 caccgcccct tcacgaacgt tgagatcgcg cgccatatcg gcaatagctg gaatataaat
    31081 ggtttgcgcc atctgaccga cggccacgag taataccaac atcaataaca aattgatgtt
    31141 tctatgcctt ttcattatca cggatgcttt tataaaaata attagaataa gtcactacgc
    31201 attcccataa atgcgggaga ggaatctatc atattgataa atgttagcca gatatacgcc
    31261 aggaatgggg aattgtttag cggcagaata tgtaaacaaa agcggcaata aatgttgccg
    31321 ggagggatgg taggtcggat aagatgcgtc agcatcgcat ccgacaccaa cccacacgct
    31381 aaatataaaa tgggagcggt tacccgctcc cctttagtgc gacttgaatc tgaattactt
    31441 caggtatttc agaacagcat caagcagttg cagtgctgca acaatgagtt tgaggataag
    31501 aatggcgata tccaccaggc tcatacgcgt ctcctgtggt tcaggagcac tgccagctga
    31561 cgtacctttc cgctgcctgt tgccagctgt tgtgcacgcg gtatctcaag gagaaacgtt
    31621 gtgctcgttg acataacaca gtgtgctcac gcggggttaa ggcgctgacg gcaccacccg
    31681 tttcagccag gacttagtgc gccgggtaac gatgcggccc ccgcataaca cattgcgtac
    31741 agtgatatta tagaagcttg ctgtataaat aaacagtaat atttggacaa aacgcaaact
    31801 gtgatcgata ctcaactatg cactaaatac gtcaaaattc gtgccgaaat tgcgcgtttt
    31861 gcgcggaaca cgtatacttt ctgtgttgac ataatacagt gtgctttgcg gttaccagcc
    31921 gcgggcggct gacgaaacct cgctccggcg gggttttttg ttacctgcag tacagtacaa
    31981 aacgtgatca acccctcaat tttcccttgc tgaaaaattt tccattgtct cccctgtaaa
    32041 gctgtgcttg tataaatatt gttaaacaca aaaccaacaa ggtccccaat ggctacttcc
    32101 atgctcaacg caaaactact accaactgcg ccatccgccg cagtggtcgt cgtgcgtgtg
    32161 gtggtggtcg tcggcaatgc gccgtaggga ctggaacaac acacgattcc aaaaccccgc
    32221 cggcgcaaac cgggcggggt ttttcgttta agcacctccc ggaaagtcgg cccagaagaa
    32281 aaggactgga gcatggcaag ttcgggcaca tcatcgacgc gtaagcgctt taccggcgca
    32341 gaatttatcg ttcatttcct ggaacagcag ggcattaaga ttgtgacggg cattccgggc
    32401 ggttctatcc tgcctgttta cgatgcctta agccaaagca cgcaaatccg ccatattctg
    32461 gctcgccatg aacagggcgc ggggtttatc gctcagggaa tggcgcgcac cgacggtaaa
    32521 ccggcggtct gtatggcctg tagcggaccg ggggcgacta acctggtgac cgccattgct
    32581 gatgcgcggc tggactccat cccgctgatt tgcatcactg gtcaggttcc cgcctcaatg
    32641 atcggcactg acgccttcca ggaagtcgac acctacggca tctctatccc catcaccaaa
    32701 cacaactatc tggtcagaca tatcgaagaa ctcccgcagg tcatgagcga tgccttccgt
    32761 attgcgcaat caggccgccc tggcccggtg tggatagaca ttcctaagga tgtgcaaacg
    32821 gccgtttttg agattgaagc acagcccgcg gtggcagaaa aagccgtcgc gcccgccttt
    32881 agcgaagaaa gcattcgtga cgcagcggca atgattaatg ctgccaaacg cccggtgctt
    32941 tatctgggcg gtggtgtgat caatgcgcct gcgagagtgc gtgaactggc ggagaaagcg
    33001 cagctgccta ccacaatgac tttaatggcg ctgggcatgt tgccaaaagc gcatccgttg
    33061 tcgctgggta tgttggggat gcacggtgtg cgcagcacca actatatttt gcaggaggcg
    33121 gatttgttga ttgtgctcgg tgcgcgtttt gatgaccggg cgattggcaa aaccgagcag
    33181 ttctgcccga atgccaaaat tattcatgtc gatatcgacc gcgcagagct gggtaaaatc
    33241 aagcagccgc acgtagcgat tcaggcggat gttgatgacg tgctggcgca gctgatcccg
    33301 caggtggaag cgcaaccgcg tgcagagtgg caccagttgg tagcggattt gcagcgcgaa
    33361 ttcccgtgtc caatcccgaa agcgtgcgat ccgttaagcc attacggcct gatcaacgcc
    33421 gttgccgcct gtgtcgatga caatgcgatt atcaccaccg atgtgggtca gcatcagatg
    33481 tggacggcgc aggcttatcc gctcaatcgc ccacgccagt ggctgacctc cggcgggctg
    33541 ggcacgatgg gttttggcct gcctgcggcg attggcgcgg cgctggcgaa cccggatcgc
    33601 aaagtgttgt gtttctccgg cgatggcagc ctgatgatga atattcagga gatggcgacc
    33661 gccagcgaaa accagctgga tgttaaaatc attctgatga acaacgaagc gctggggctg
    33721 gtgcatcagc aacagagtct gttctacaag cagggcgttt ttgccgctac ctatccggga
    33781 aaaatcaact ttatgcagat tgccgccgga ttcggcctcg aaacctgtga tttgaataac
    33841 gaagccgatc cgcaggctgc attgcaggaa atcatcaatc gccctggtcc ggcgctgatc
    33901 catgtgcgca ttgatgccga agaaaaagtg tacccgatgg tgccgccagg tgcggcgaat
    33961 acagaaatgg tgggggaata agccatgcaa aacacaactc atgacaacgt gattctggaa
    34021 ctcaccgttc gcaaccatcc gggcgtaatg acccacgttt gtggcctttt tgcccgccgc
    34081 gcttttaacg ttgaaggcat tctttgtctg ccgattcagg acagcgacaa aagccatatc
    34141 tggctactgg tcaatgacga ccagcgtctg gagcagatga taagccaaat cgataagctg
    34201 gaagatgtgg tgaaagtgca gcgtaatcag tccgatccga cgatgtttaa caagattgca
    34261 gtgttttttc agtaaggagc gacattaaac cgctcggggg agttactgat gagcgatttt
    34321 tgatagatcc gtgcagatat atagggtgaa attgtgccag agaccatctc aggcacaatt
    34381 tattagccca ggacgttaat ttaagtcgca gcaccggtaa cctggggggg acagttaggg
    34441 ctaaaatttt cagcgatggt gttaaattct gattgctgtc tataccagtg gtcatgacca
    34501 gcattctatt aattaatggt tcatcgtgag gtatattatg gcaagtccta taaacagcgg
    34561 aataatgatg gcaaatctct gcccttccac ctttttggat aaaaatagaa atgtcgctgc
    34621 tgagcttgat attaaaaata acgagaaaaa atattcacct ggcagtaatt tcgcaaaatg
    34681 gatgttgcag gaaataaagc gcttaatact aaacatcatg tccggttctc ggagtattaa
    34741 caccgatatt cttgactatt tccaccctat gccaggcacg gaaaataatg gtaatcgtac
    34801 ctgggtggcg gcaactggag aggatgaata tatcgaaatc aaacaaacag gtgataagtc
    34861 ttttaatatt acactggttg gcagagataa accttcacga aaagaaattc catattcagg
    34921 agtggctgta gcgactatta ttaaatcgtt gtctgaaaaa acttctgcgt tagaaacaca
    34981 ttccgctgac acggttttga g
//
LOCUS       DBFIFS010000027        33743 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.027, whole genome shotgun sequence.
ACCESSION   DBFIFS010000027 DBFIFS010000000
VERSION     DBFIFS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.027"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..692
                     /locus_tag="N7A07_001212"
     CDS             <1..692
                     /locus_tag="N7A07_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU2475910.1"
                     /translation="LLKRIMSINDNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDG
                     IKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINK
                     KHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNG
                     GRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSAGPEITTPWKSYQHEKNRNGLM
                     PLIRRRTMLQL"
     gene            882..995
                     /gene="yhiY"
                     /locus_tag="N7A07_001213"
     CDS             882..995
                     /gene="yhiY"
                     /locus_tag="N7A07_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU2475911.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1058..2125
                     /gene="yhiI"
                     /locus_tag="N7A07_001214"
     CDS             1058..2125
                     /gene="yhiI"
                     /locus_tag="N7A07_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2475912.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2122..4857
                     /gene="rbbA"
                     /locus_tag="N7A07_001215"
     CDS             2122..4857
                     /gene="rbbA"
                     /locus_tag="N7A07_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU2475913.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEASPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4857..5981
                     /gene="yhhJ"
                     /locus_tag="N7A07_001216"
     CDS             4857..5981
                     /gene="yhhJ"
                     /locus_tag="N7A07_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2475914.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6054..6329
                     /locus_tag="N7A07_001217"
     CDS             6054..6329
                     /locus_tag="N7A07_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU2475915.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6326..6685
                     /locus_tag="N7A07_001218"
     CDS             6326..6685
                     /locus_tag="N7A07_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU2475916.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6735..7595)
                     /locus_tag="N7A07_001219"
     CDS             complement(6735..7595)
                     /locus_tag="N7A07_001219"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HGU2475917.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7627..7896)
                     /locus_tag="N7A07_001220"
     CDS             complement(7627..7896)
                     /locus_tag="N7A07_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU2475918.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(7886..9394)
                     /locus_tag="N7A07_001221"
     CDS             complement(7886..9394)
                     /locus_tag="N7A07_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU2475919.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9387..10745)
                     /locus_tag="N7A07_001222"
     CDS             complement(9387..10745)
                     /locus_tag="N7A07_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU2475920.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10822..11103)
                     /locus_tag="N7A07_001223"
     CDS             complement(10822..11103)
                     /locus_tag="N7A07_001223"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2475921.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(11100..11573)
                     /locus_tag="N7A07_001224"
     CDS             complement(11100..11573)
                     /locus_tag="N7A07_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU2475922.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11598..12344)
                     /locus_tag="N7A07_001225"
     CDS             complement(11598..12344)
                     /locus_tag="N7A07_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2475923.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12543..12944)
                     /gene="nikR"
                     /locus_tag="N7A07_001226"
     CDS             complement(12543..12944)
                     /gene="nikR"
                     /locus_tag="N7A07_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU2475924.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(12950..13756)
                     /gene="nikE"
                     /locus_tag="N7A07_001227"
     CDS             complement(12950..13756)
                     /gene="nikE"
                     /locus_tag="N7A07_001227"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU2475925.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(13753..14517)
                     /gene="nikD"
                     /locus_tag="N7A07_001228"
     CDS             complement(13753..14517)
                     /gene="nikD"
                     /locus_tag="N7A07_001228"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU2475926.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14517..15350)
                     /gene="nikC"
                     /locus_tag="N7A07_001229"
     CDS             complement(14517..15350)
                     /gene="nikC"
                     /locus_tag="N7A07_001229"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU2475927.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15347..16291)
                     /gene="nikB"
                     /locus_tag="N7A07_001230"
     CDS             complement(15347..16291)
                     /gene="nikB"
                     /locus_tag="N7A07_001230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU2475928.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16291..17865)
                     /gene="nikA"
                     /locus_tag="N7A07_001231"
     CDS             complement(16291..17865)
                     /gene="nikA"
                     /locus_tag="N7A07_001231"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2475929.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(17976..18563)
                     /gene="acpT"
                     /locus_tag="N7A07_001232"
     CDS             complement(17976..18563)
                     /gene="acpT"
                     /locus_tag="N7A07_001232"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU2475930.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18565..19794)
                     /locus_tag="N7A07_001233"
     CDS             complement(18565..19794)
                     /locus_tag="N7A07_001233"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HGU2475931.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19791..20522)
                     /locus_tag="N7A07_001234"
     CDS             complement(19791..20522)
                     /locus_tag="N7A07_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HGU2475932.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(20522..20986)
                     /locus_tag="N7A07_001235"
     CDS             complement(20522..20986)
                     /locus_tag="N7A07_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HGU2475933.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(20983..22152)
                     /locus_tag="N7A07_001236"
     CDS             complement(20983..22152)
                     /locus_tag="N7A07_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HGU2475934.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22154..22738)
                     /locus_tag="N7A07_001237"
     CDS             complement(22154..22738)
                     /locus_tag="N7A07_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HGU2475935.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(22735..25053)
                     /locus_tag="N7A07_001238"
     CDS             complement(22735..25053)
                     /locus_tag="N7A07_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HGU2475936.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25022..25627)
                     /locus_tag="N7A07_001239"
     CDS             complement(25022..25627)
                     /locus_tag="N7A07_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HGU2475937.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(25624..26046)
                     /locus_tag="N7A07_001240"
     CDS             complement(25624..26046)
                     /locus_tag="N7A07_001240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HGU2475938.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(26050..27726)
                     /locus_tag="N7A07_001241"
     CDS             complement(26050..27726)
                     /locus_tag="N7A07_001241"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2475939.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(27717..28070)
                     /locus_tag="N7A07_001242"
     CDS             complement(27717..28070)
                     /locus_tag="N7A07_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HGU2475940.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28057..29418)
                     /locus_tag="N7A07_001243"
                     /pseudo
     CDS             complement(28057..29418)
                     /locus_tag="N7A07_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            complement(29415..29996)
                     /locus_tag="N7A07_001244"
     CDS             complement(29415..29996)
                     /locus_tag="N7A07_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475941.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(30001..30252)
                     /locus_tag="N7A07_001245"
     CDS             complement(30001..30252)
                     /locus_tag="N7A07_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2475942.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30264..30521)
                     /locus_tag="N7A07_001246"
     CDS             complement(30264..30521)
                     /locus_tag="N7A07_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGU2475943.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(30496..31317)
                     /locus_tag="N7A07_001247"
                     /pseudo
     CDS             complement(30496..31317)
                     /locus_tag="N7A07_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(31314..32036)
                     /locus_tag="N7A07_001248"
     CDS             complement(31314..32036)
                     /locus_tag="N7A07_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HGU2475944.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(32077..33135)
                     /locus_tag="N7A07_001249"
     CDS             complement(32077..33135)
                     /locus_tag="N7A07_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2475945.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33204..33581)
                     /locus_tag="N7A07_001250"
     CDS             complement(33204..33581)
                     /locus_tag="N7A07_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2475946.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
BASE COUNT         7878 a         8975 c         9185 g         7705 t
ORIGIN      
        1 atttgttaaa acgcataatg agcatcaatg ataacgttca tgtattagct ggaaatcatg
       61 aaacgaattg caatggtaac tatatgcaga acttcacgcg tatgaaacca cttgatgagg
      121 atacctacga cgggataaaa gattatcctg tatgtttcta tgactccaaa tacaaaataa
      181 tggcaaatca tcatgggata acttttgatg atcagcgaaa acgttacatt ataggaccga
      241 taacggtctc tattgatgaa atgaccaatg ctctcgatcc ggtcgaatta gcggcaatta
      301 ttaataaaaa gcatcatact ataattaata gtaaaaagtt taaaactagc cgggctatct
      361 cttgtcgatc ctttaatcgt tatttttctg tttcgacaga ttacagacct aaacttgaag
      421 cactattagc atgctcacaa atgttaggta taaatcaagt tgttgcacat aacggtaatg
      481 gtggaagaga atgcataggt gaaacgggga cagttttagg tcttaacgcc cgtgacagta
      541 aacatacggg aagaatgttc agcatgcata attgccaaat taaccctagt gcggggcctg
      601 agataaccac tccctggaag tcttaccaac atgaaaaaaa cagaaatgga cttatgcctt
      661 tgattagaag gcgcacaatg ttacagctct gaaccataga caacatgaat attaatgtta
      721 ctatctggtt gagactgttg ataaaacgta ataaggaatg ctctgttcct gcaactgaac
      781 aggcgtgaaa cgctatccaa caggatggat accgttttgt cagccagttt tatagatcac
      841 acttatgagt atgcgtgttt catttacggc caggaatgct gatgatgaca acattactac
      901 ccgttttcac taagccttcc ccgttggcgc tcaatgctct gcgcgctggt cgtatttgtc
      961 gtttccttct tatcccggat ggaagaatcc gctgaacgtc gttcggcggc agttgtaaca
     1021 cgcgtcgttt gcttacaggg attatggcgg gtcgattatg gataagagta agcgccatct
     1081 ggcgtggtgg ggtgtcgggt tactggcggt ggctgctatc gtagcgtggt ggctgttgcg
     1141 cccggcaggt gtgccggaag gctttgccgt cagcaatggg cgcattgaag cgacggaagt
     1201 ggatatcgcc agcaaaattg ccgggcgtat cgacaccatt ctggtgaaag aaggccagtt
     1261 tgttcgcgaa ggcgaagtgc tggcgaagat ggatactcgc gtgttgcagg agcagcgact
     1321 ggaagccatc gcgcaaatca aagaggcaca aagcgccgtt gccgccgcgc aggctttgct
     1381 ggagcaacga caaagcgaaa ctcgcgccgc acagtcgctg gttaatcaac gccaggccga
     1441 actggactcc gttgccaaac gtcatacgcg ttcccgctcg ctcgcccaac gaggggctat
     1501 ttctgcgcaa cagctggatg acgatcgcgc cgccgctgag agcgcccgag ctgcgctgga
     1561 atcagcaaaa gcccaggttt cggcttctaa agccgctata gaagcggcac gcaccaatat
     1621 cattcaggcg caaacccgcg tcgaagccgc acaagccact gaacggcgca ttgccgcaga
     1681 tatcgatgac agcgaactga aagccccgcg tgacggacgc gtgcagtatc gggttgccga
     1741 gccaggggaa gtgctggcgg caggcggtcg ggtgctgaat atggtcgatc tcagcgacgt
     1801 ctatatgacc ttcttcctgc caaccgaaca ggcgggcacg ctgaaacttg gcggtgaagc
     1861 ccggctgatc ctcgatgccg cgccagatct gcgtattcct gcaaccatca gttttgtcgc
     1921 cagtgtcgcc cagttcacgc caaaaaccgt cgaaaccagc gatgaacggc tgaaactgat
     1981 gttccgcgtc aaagcgcgta tcccaccgga attactccag cagcatctgg aatatgtcaa
     2041 aacgggcttg ccgggcgtag cgtgggtgcg ggtgaatgaa gaacttccgt ggcctgacga
     2101 cctcgtggtg aggttgccgc aatgacgcat ctggaactgg ttcccgtccc gcctgtcgcg
     2161 caactggcgg gcgtgagcca gcattatgga aaaaccgttg cgctgaacaa tatcacactc
     2221 gatattccgg cccgctgtat ggtcgggctg attggtccgg atggcgtcgg gaagtcgagt
     2281 ttattgtcat tgatttccgg tgctcgtgtc attgagcagg gcaatgtgat cgtgctgggc
     2341 ggcgatatgc gcgacccgaa gcatcgccgc gacgtctgcc cgcgcatcgc ctggatgccg
     2401 caggggctgg gcaaaaacct ctaccacacc ttgtcggtgt atgaaaacgt cgattttttc
     2461 gctcgcctgt tcggtcacga caaagcggag cgggaagtgc gaatcaatga gctgctgacc
     2521 agcaccgggt tagcaccgtt tcgcgatcgt ccggcaggga aactctccgg cgggatgaag
     2581 caaaaactgg ggctgtgctg tgcgttaatc cacgacccgg aactgttgat tcttgatgag
     2641 cctacgacgg gggttgaccc gctctcccgc gcccagttct gggatctgat cgacagtatt
     2701 cgccagcggc agagcaatat gagcgttctg gtcgccacgg cctatatgga agaggcggaa
     2761 cgcttcgact ggttggtggc gatgaatgcc ggagaagtgt tggcaactgg cagcgccgaa
     2821 gagctgcggc agcaaacgca aagcgccacg ctggaagaag catttataaa tctgttaccg
     2881 caagcgcaac gccaggcgca tcaggcggta gtgatcccac catatcaacc tgaaaacgca
     2941 gagattgcca tcgaagcgcg cgatctgacc atgcgttttg gttccttcgt tgccgttgat
     3001 cacgtcaatt tccgcattcc acgcggggag atttttggtt ttcttggttc gaacggctgc
     3061 ggtaaatcca ccaccatgaa aatgcttacc ggattgctgc ccgccagcga aggtgaggcg
     3121 tggctgttcg ggcaaccggt tgatccaaaa gatatcgata ctcgccgtcg ggtgggctat
     3181 atgtcgcagg cgttttcgct ctataacgag ctcaccgtgc ggcaaaacct tgagttacat
     3241 gcccgtttgt ttcacatccc ggaagcggaa attcccgcaa gagtggctga aatgagcgag
     3301 cgttttaagc tcaacgacgt tgaagatgtt ctgccggagt cattgccgct cggcattcgc
     3361 cagcggcttt cgctggcggt ggcggtgatt catcgcccgg aaatgttaat cctcgatgaa
     3421 cctacttctg gcgtcgatcc ggtggcgagg gatatgttct ggcagttgat ggtcgatctc
     3481 tcgcgccagg acaaagtgac tatcttcatc tccacccact ttatgaacga agcggaacgc
     3541 tgcgaccgta tctctctgat gcacgccgga aaagtgctcg ccagcggtac accgcaggaa
     3601 ctggttgaga aacgcggagc cgccagtctg gaagaggcat ttatcgccta tttgcaggaa
     3661 gcggcaggac agagcaacga agccgaagcg tctcccgtga tacacgacac cacacacgcg
     3721 ccgcgccagg gatttagcct gcgccgtctg tttagctaca gccgccgcga agcgctggaa
     3781 ctgcgacgcg atccggtacg ttcgacgctg gcgctgatgg gaacagtgat cctgatgctg
     3841 ataatgggtt acggcatcag tatggatgtg gaaaacctgc gctttgcggt gcttgaccgc
     3901 gaccagaccg tcagtagcca ggcgtggacg ctcaatctct ccggttcccg ttactttatc
     3961 gaacagccgc tgcttaccag ttatgacgag cttgatcgtc ggatgcgtgc gggcgatatc
     4021 acggtggcaa ttgagatccc gcccaatttc gggcgcgata tcgcgcgtgg tacgcctgtg
     4081 gaactcggtg tctggatcga cggtgcgatg ccgagccgcg ccgaaactgt aaaaggttac
     4141 gtgcaggcca tgcaccagag ctggctacag gatgtggcga gccgacaatc cacaccctcc
     4201 agccaaagcg ggctgatgaa tattgagacg cgttatcgct ataacccgga cgtaaaaagc
     4261 ctgccagcga ttgttccggc ggtgatcccg cttctgctga tgatgatccc gtcaatgcta
     4321 agcgccctta gcgtggtgcg ggaaaaagag ctagggtcga ttatcaacct ttacgtgacc
     4381 cccaccaccc gtagcgaatt tttgcttggc aaacagctgc cgtacatcgc gctggggatg
     4441 ctgaactttt tcctgctctg cggcctgtcg gtgtttgtgt ttggcgtacc gcataaaggc
     4501 agtttcctga cgctcaccct ggcggcgctg ctgtatatca tcatcgccac cggaatgggg
     4561 ctgctgatct ccacctttat gaaaagccag attgccgcca ttttcggtac ggcgattatc
     4621 acgttgatcc cggcgacaca gttttccggg atgatcgatc cggtagcttc gctggaaggg
     4681 ccgggacgtt ggatcggcga ggtttacccg accagtcatt ttctgactat cgcccgcggg
     4741 acgttctcga aagcgctgga tctgactgat ttgtggcaac tttttatccc gttgctgata
     4801 gccataccgc tggtgatggg cttaagcatc ctgctgctga aaaaacagga gggatgatgc
     4861 gccatttacg caatattttt aatctgggta tcaaagagtt gcgcagtcta ctcggtgata
     4921 aagcgatgct gacattgatt gtcttctcgt ttacggtgtc ggtgtattcg tcagcgaccg
     4981 tcacgccagg atcgttgaac ctggcaccga tcgccattgc cgatatggat caatcgcagt
     5041 tatcgaaccg gatcgttaac agcttctatc gcccgtggtt tttgccaccg gagatgatca
     5101 ccgccgatga gatggatgcc ggactggacg ccgggcgtta tacctttgcg ataaatattc
     5161 cgcctaattt tcagcgtgat gtcctcgccg gacgccagcc agatattcag gtgaacgtcg
     5221 atgccacgcg catgagccag gcatttaccg gcaatgggta tatccagaat attatcaacg
     5281 gtgaagtgaa cagctttgtc gcgcgctacc gtgataacag cgaaccgttg gtatcgctgg
     5341 aaacccggat gcgctttaac ccgaaccttg atcccgcgtg gtttggcggg gtgatggcga
     5401 tcatcaacaa cattaccatg ctggcgattg tattgaccgg atcggcgctg atccgcgagc
     5461 gtgaacacgg cacggtggaa cacttactgg tgatgccgat aacgccgttt gagatcatga
     5521 tggcgaagat ctggtcgatg gggttggtgg tgctggtggt atcgggatta tcactggtgc
     5581 tgatggtaaa aggcgtgttg ggcgtaccga ttgaaggctc gatcccgctg tttatgctgg
     5641 gcgtggcgct tagtctgttt gccaccacgt caatcggcat ttttatgggg acaatagcgc
     5701 gttcaatgcc gcaactgggg ctgctggtga ttctggtgct gctgccgctg caaatgcttt
     5761 ccggtggttc cacgccgcgc gaaagtatgc cgcagatggt gcaggacatt atgctgatca
     5821 tgccgacgac acactttgtt agcctcgcgc aggccattct ctaccgtggt gccggattcg
     5881 aaatcgtctg gccgcagttc ctgacgctga tggcaattgg cggcgcgttt ttcaccattg
     5941 cgctgctgcg attcaggaag acgattggga caatggcgta aggaagggaa acttgacttc
     6001 atactgtgat gtcatactat tgatgtcata aagtgaagtc ataaggacat gccatgagaa
     6061 ccaaggtaca ggctttacgg aagaaacaaa aaaatacatt ggatcaaata tttaaaactc
     6121 ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca ttaggcggag
     6181 aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc gttgcgtgtt
     6241 tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc gtgcgtgact
     6301 ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact ctatggagat
     6361 tgccgggcaa cctgctgtta tcacttacgt gccagaactg aatgcatttc gtggcaaatt
     6421 cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc tgcaaaaaga
     6481 aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta ttgagccgta
     6541 tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc tgagtgaacg
     6601 ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta ttgaaacact
     6661 gaatgagcgt ttgaatcatt tataacttat agcggcccaa caatttctgt tgcgccgcta
     6721 tatttttgtg gctcttaaga tgcctttcta accaaccgat gcccctcatt catcatcagg
     6781 attttgctat acacatcttc ctcgacggcg ttttttgctg atgccatgac tcgtgccaga
     6841 ttcgcctcgt tgtgattatt cacatacgct ttgccgacgg cggtgataaa cgctttgcgc
     6901 agatcgctgg cgatatttac tttagcgacg cgataattaa caaagctgcg taatatctcc
     6961 ggggcgatac cggagccgcc gtgaataacc aacggcaccg gacagacttc ggcaatgcgc
     7021 ttcagcaggg ggatatcgat acgcggtata tcgtccaggc catgaacgtt gccaatagaa
     7081 actgccagca tgtcgcaacc ggtacgctcg acaaaggtct tcaccttttc cggttccgtt
     7141 ttgcaatcgg cttcgctgac gtgatcatct tctttgccga gaatcgcccc cagctccgct
     7201 tcaaccggta cgccatagga tttacagaaa tcgacagctt cctgggtgaa agcgatattc
     7261 tcttcaaaag agaatgcggc accgtcaatc atgaccgagg taaagcccgc gcgcaccgcc
     7321 tgtttaacat cctcctgggt tttcccgtga tccagatgaa gactgaccgg aacatccatt
     7381 ttatcggcgt gacgtttcac gatttcataa atgtaatcgt atcccgagag ttgtgcattg
     7441 gttggcgcaa tctgaatgaa gttgggtaat cccgaacgct caatagcgtt taaaattgaa
     7501 attgtcgtct ccagattggt ggtattaaat gcgcccgcta atacccgttt ttcctgaatg
     7561 cggtcgagca gtatccgtcc attaactaat ggcatattga aaacctcatc ttttaatatc
     7621 agttaattat tcagcttcac cgaagcggtc gcgaaataag tcggtgagag tctgtatggc
     7681 ctcgttttca tcatcaccgt cgcagcgtat ttccaccgtt tttccgcctt ttattccggc
     7741 gcgcataata ttcatcatgc tggtggcatt aatatcatta ccctcatagt gcagcgtcac
     7801 tttacagcta tattttttgg cggctttcgc cagacaggcc gccggacgtg catgcaggcc
     7861 agtactgttt ctgacctcca cggttttatt caacatagtg ttcctctcga tgatgtaatg
     7921 gcatcacgcc ttcttgttgt aatttcaact gataccagct tttccatttg tgctgataac
     7981 gctgtaattg atggtatctc gcccgattcg gtaacaaacg ttgggctata gcgactggcg
     8041 gcggtggaat atccggtgcg gcggttaata atgctccgac agaaaccgcc tcgttaaatg
     8101 caattgcttc aaccggacgc tgtaaaatat ccgcctttaa ttgcagccaa agaggattat
     8161 gggttgccgg accgatcact tttaacgcgc tggtgccagg cacgttaaaa caggcgaagc
     8221 agttggcaaa ttccatggtt aatcccagtg atacgctggc aattaacatg tcgatactta
     8281 acgtatcgcc aagcccataa aataatccac gggtatggcg atttttatag ggcgaacccg
     8341 aaccgcgaag atgtggaata aagacgggaa tgtcatcgag caaccaattc cccgccagat
     8401 aatcagcatg tccgcgagta agtgatgcgg cgatctcctc atcggttagc cggaacgtgt
     8461 tgcgcagcca ctccagcgcg aaaccgccca cgggcagcga cgcaaacagg gtgaactggt
     8521 tggcaagtga gtaacagccg tttgccagct tgtcacgttt ggcctgttca tccagcgtcg
     8581 gttgtgtatc cagttgcaga atgccttccg tggtccccgt cgagttaagg atatcgcctg
     8641 gcatcatctg caacgctcgc gcacccacca tatggtcatg tccggccagc gtcacgctga
     8701 ccggttgcga gaacccaagc gtcttgcaaa gtgactcgct gacccatccg gcgtgctcgc
     8761 ctggctgcac caatggcgca aatgccgtac agggaacgtg taacaacgcc gccgctttcg
     8821 ctgaccattc gcctttgcgc acgtccagac acagcgtgcg gctggctaag gtgatatccg
     8881 tgtgctgttc gccagtcagc cgccagagca gcacttccgg cgcatgtagc cagcgcaggc
     8941 cgcggcgatt acgcagcggg taatgttcca gcaaccagcg cattttgaag gcagagtaat
     9001 tgctgtgtag tggtagaccg cagatgtcat aaagtgccgc actgtctgcc tcgctaagcg
     9061 ttgccagata ctcttcaccg cgacggtcat accatgccag cattggcgtc aggatctcgc
     9121 catgctcgtc aaggaacacg cctgattcgc caaaactggc aatgctgatc cgtctgactg
     9181 gcagcggcga agcggcgttc agttgcgcta tcgcctggcg gacctcctgc cagagggcgt
     9241 cgatatcgaa atcgacatcg ccctgtgggg agatctgctt tgccgtcacg aatttatgcg
     9301 cgcccaacgt cgttgcgtcc aggcaggaaa agcaggtgac tttgcaattg gtggtgccaa
     9361 tatcgataac gatagctgcg ctgttatcag gcataaaccg tgctccgcgt cattcggaaa
     9421 atgccataca gtaccagggc gataaccggc accagaccaa tggcgatgag attgccgttg
     9481 aagatttcca gcagccagaa gcggaacggg ttaccgccat caagaaaact ggaaacctgg
     9541 ccgctggagc cttcggcaaa tttgaagtta acgtctttcg ccattccggt aataaacggc
     9601 gcgattttgg tagcaatcca taggtcggca ataatcaccg ggatcgccgc cagaacggtg
     9661 cggaaaatat tgccacggct ggctaaagca atcatcgacg ccattaccgc caggttggca
     9721 aggtcgccaa gcggcaggac ttcattaccc ggaaggacaa acgcgataaa gaccgagatt
     9781 ggcgttagca gcagggcggt ggagataatc gccggattac ctacggcaac ggcgatatcc
     9841 aggccgatat agagatcgtc acggtcaggg tatttggcat tgagatactt tttaatagct
     9901 tcagacagcg gtaataaacc ttccatcagg atgcgtacca tgcgtggcag gatgaacatc
     9961 acaccgccaa tgctgatgcc aagcagcaat acttttttga aatcgtatcc ggcaattacg
    10021 ccgagcagaa tgcccagaat agtgccaacc atcatcggtt cgccgaagat gccaaacttt
    10081 ttctgtacgg tttccgggtc gatatgaata cgattgaggc cagggatatg gtcgatgatt
    10141 ttgtcgacca gcagaccgac cgggaagaac accaccgaag agagcgtcgg caatgagatc
    10201 ccttccagtc caaagtattt ttgtaccagt ggcgcagacc agtcggcgag tttaagtact
    10261 accaccgcag caatcgccgc cgccagcaaa ccaaagaaca ggctgccggt gctgtaataa
    10321 accaacgtac cagcaagggc aaaatgccag tagttccaca tatctacgtc aacggtacgg
    10381 gttttattta acgccagcat cgccacgtta atcagaatgg tcaggggaat aacaaacggg
    10441 gcaattggcg aaccccatgt aatagcggaa agcggcggcc agcctaaatc taccaccggt
    10501 aaattaattc cggtgcgttc aaccatcgcc tgggccgctg gcccgacatt gctggtcaga
    10561 atatcgaaaa tggcataaat accgacaaaa ccaatgccga ctgttaatga cgaacgtaag
    10621 gctttagcgg gttttacttt aaaacagaga gcaagaataa ataacaccaa cggcaacatt
    10681 accgtcgggc caaatcccaa tatatattgc acaatattat agagggtatg cgcgatatcg
    10741 ttcataataa acctcttttt caggcgagag cgcacctcgc gttagcgcct gatggagtgg
    10801 gttgaaatta attattatta attacttttt aaggatagaa acaatttgcg ccagtacttt
    10861 ttcttcgcca ataccggtaa taatcggcaa gccgttcacc accgggacgt cgagttcata
    10921 aggcacttta gttgttgaaa ctaccagtgc tacgccgtca gtatttccag gtaaagaggc
    10981 aacgttggtt tgggaataat tgacgttaag accttgctct ttgcaatatt ccatgacttt
    11041 ttctgttacc gccgtggatg tggcaatacc cgtggcgcag acaaataaaa tggtttgact
    11101 catatttcac ctcgttcttt cattttgttt aagattactg tgtgtatttc tgttgtttcc
    11161 atcgcaagca gggcttgcat aaagggcgta tcctgaatca tttccataat ccagcccagg
    11221 gcatttaatt gtttattgct ttcgcttaag gcgagcaaaa aaatcaccct gacaggtacg
    11281 gggtcaggat cgccgcccat atcggcaaac tctaccggtt ccggcaggat gccgacggca
    11341 attgcattgt gcctgacatg tttatggtcc gtgtgcggaa tagcgacgca cggtggttcg
    11401 gtaggcagcc ccgttgggta cgtcgcctca cgtttcagta cgtgatcggc aaaatcaggg
    11461 acgaccagct cggcggcaac caacggttgc gctaactggc ggatgatctg ttcaacgctt
    11521 aaccctgccg gaagtcgttg aatataattt tcctgaaaat gtatgccttg cattttattc
    11581 ctcttaaaaa agtgtactta actcacttcc cgccgttgca gtacggtgcc aagataatat
    11641 ttatttcctt ttaaccagac gttgccaaac tcaatgggta atcctcggga gaaaaagact
    11701 aactgctcca gatgcaaaac gggtgcgtct tcactgatat ctaaaaaatg accacgttca
    11761 ttaccaatta atcgcgcggc atagcgactt tccgagtaac gaatttttct ttgcgacaaa
    11821 ctttctattg ttggaaataa attgtgttga ttaaaatcga tttcagcaat accggggcat
    11881 agctcaatat tgatacggtt ctctatcagc atcgcttttt catcaccaat tgagcgtaag
    11941 cgttcaaggt aaagaatatc ctgaccgggc gttattctta atttctctgc cacataacga
    12001 ttagccgatt caatccgtga cgtaataact tcagtggtaa aatgtatttt ctggctttcc
    12061 agcgattccg cgaatgacaa taatccttca cctaacggat atgccacgtt ctcttttttg
    12121 acaaatgttc ccttaccctg gacctgtatt aacacgcctt cttttaccag ttgggcgacc
    12181 gcttttttaa cggttccccg actcacgtcc agcatatcca taatctggta ttccgaagga
    12241 atgcgatcgt cttccaccag atctccggta taaatacttt ctcttatcca ctccaccact
    12301 tgttgataga gcggtatttg tgacgactta cttaatgatt tcatggcgcg cacctgaaca
    12361 tttacagttg tctatggaca atcatggaca acttcaagcg tatagtaggt gaacgggaga
    12421 aaaagaggat cgaagcgtct cacattttga gggaccaaat cgtgatcaaa aaaatgtgac
    12481 gtgagtcgat atttgtcaga agtttactgc gccgtaagga aataacggcg cgttggtagg
    12541 tgttaatctt ccttcggcag gcactgcaaa tgcccgtgcc gcacgccgcg ctgggcgata
    12601 acgtcatcgg caaaatgctg cacgtcaccc atgtcacctt tcaacacggc gatttccaga
    12661 cagtcgtcgt ggttgatgtg cacatgtagc gtggcgacgg agaggtcgtg gtgatgatgc
    12721 tgggtggaaa caatacggct ggctaagtcg cgtttttcgt gttcatacac atacgacagc
    12781 accgcgaaac cttgtgtgcc gtgctgctgg gtagcttcct gcgccagggc gctacgcaga
    12841 atatcgcgga tagcttcgga acggttgtta taaccacgac gctggctcag gctgtccagc
    12901 gtctccagta aatcgtcatc aagcgtgatg gtgactcgtt gcatttgcgt taaacctttt
    12961 ctgtggcgcg acggcgcacg gggaatgcgg gtaatatcgc gttttgtagc acacgtccgg
    13021 cgtcagagga aaaggttaat ttctctccca ccgcctgggt ttcgacgatt tgtccgttgt
    13081 ccatcaccat cactcgctgg caaaagcgtt ccaccaggcg taagtcatga gtgatgaaca
    13141 ggcaggcagt gccaaactgt tgttgtagct ttttcagcag gcgaatgacg cccgcctgta
    13201 acacgagatc aaggttagaa acggcttcat ccagaatcag caatttcggt tcgaccgcca
    13261 gcgcgcgtgc caggcagacg cgctggagct ggccgccgct taactgcggt gggcgcttgt
    13321 cgagcacgct gtcgtcgaga tcgaccgcgt gcagcatttc gctggcacgc gccagttgtt
    13381 cggatttttt cagcgatagc aggtggcgca tcggttcccg caggatctcg cgcacggttt
    13441 tgcgcggatt cacggcgctg atggagtcct gaaataccat ctgaatatcg cggcggaacg
    13501 ctttacgctg ggcgcgattg agctttgcca acgattcgcc acgccagcta atatttccct
    13561 gtgagggcga ttctaagcct accagtagcc gcgagagagt gctttttccg cagccgctgc
    13621 gtcccagcag ggcgacggtt tcaccgcttt tcagggtcag ggaaacgtta ttcagcaccg
    13681 cctggtgctg atgttttccg ctaaacccac cgtgggcata gtgatgggaa aggtcggaga
    13741 cgttaagtaa agtcatgatg ccagctccat accgtagagg gcgagatgag cggaaaccag
    13801 gctgcgcgtt accgcatgtt tgggggcgtt aaacagcgtt tcgacatcgc cctgttcgac
    13861 aatcttaccg tcagacatta ccgctacgtc atccgccaga cgcgccacca cgcccatatc
    13921 atgggtgacc agcagcatcc ccggcgcctg tttttgcata atgctttcca gcagatcgag
    13981 aatgcgtgcc tgtgctacca cgtcgaggtc ggtggttggt tcatcggcga tgataaacgg
    14041 tgattcgcaa agcaccgcca tcgcaatcat catgcgctgc aacatgccgc cgctcatctc
    14101 gaacgggtac agcttcagca cgcgtgcggc gttttccagc cccaccgctt ctatggcagc
    14161 ggtaagcgta gcgtcatcgg cgggtttccc cagcgccagg cacgtctccc gcgcgtgggt
    14221 gtgcatggtg tgcagtggat taaaggcgct gcgcgggttc tgcatgatgg tggcaatttt
    14281 gatgccgcgc agggcgcagg gagaaaccgg tttgccatcg gctaaaattt cccccgccgt
    14341 ctggcgaacg cctgcgggca gaattcccag cgtcgcggcg caggttaatg atttcccgct
    14401 gccgctaccg ccgactaacg ccagcacgcg cccgcgtttc agggttaacg acacgccgtg
    14461 caccagcggc tgcgcggcct gtagcgcgat attacgtagt tcaatctgtt gcggcattag
    14521 tgtgcgtgct ccgtcaccag atgaggatcc agatgatcgc gcagtgcgtc acccaccagg
    14581 ttaaaggcca tcacgctgat aaacagcgcc agccccggcc agaacatttg cagcggctgg
    14641 gtccagatat actggcgcgc gtcgttaatc atcacgcccc attcggcggt cggcgcggtc
    14701 acgccgaggc cgaggaagga catcccggca acgtgcagca tcatatggcc gatatccagc
    14761 gttgccagca ccagcagcga agggatcacc gcacctgcca gatgatcaac aaacactcgc
    14821 acatggcccg cgccagaaag ccgtgatgcc agcacaaact cgcgctggcg cagtgaaatc
    14881 accaggctgc gcaccatgcg tgcataccac gcccagtgcg acagggcgat ggcgataatt
    14941 acgttggtca gcccagtgcc gagcacgcca accataaaga atgacagaat cgaggtcggg
    15001 aaggtcataa acatatcggc gacgcgcatg gtagcctgat caacgcgccc gccaatcagc
    15061 ccggcgctgc cgccaataac cagccctaat gtcagcacca gcagcaggca ggccattacc
    15121 gaaccgagcg acacacgggt cgctgccatc agccgcgaga aaatatcgcg ccctaagtga
    15181 tcggtgccca accagtgctg cgcatccggc gaaagcaggc gcgacggcaa atcaatcgcc
    15241 tgggggtcat acggcagcca ccactggctg gtgagcgcaa tcagcgccag cagggcgata
    15301 atgatcagcg ccaggcgtac cgaccagcgg gaagagagga aaaagttcac gcgtgcgctc
    15361 cttcatgacg acgaatgcgt gggtccagtg cggcgttgag caaatcgaca atcaaattac
    15421 agaccacaaa aaccaccacc atcatcagcg taaagcactg gatcaccgga tagtcacggt
    15481 taaaaatcgc cgacaccgca tagcgcccga cgccgggcca ggtaaagatg ttttcgataa
    15541 tcatcgtccc gccaatcagt tcgccaatgt gcatccccac ggcggtgatc atcggcagcg
    15601 aggcattgcg cagaatatga cgacgctcgg tctgtttgtc attcaggccg cgtagccgcg
    15661 cccaggtgac gtgacgctga ccggcgacgt ccagcatact ggcgcgcagc agccgtgcgt
    15721 taatcgccag cgacataaag gcaatggaaa ccgcaggcaa aatgatgtgc tgccagccgc
    15781 cgtagcccat cgcgggtagc cattgcagat acaccgaaaa cgccatcacc agtaaaaatg
    15841 ccagccagaa gttgggcatc gacacgccga gaaacgcgat gaaacgtacg gcgaaatccg
    15901 gcagacggtc acgatggcgc gccgcccaga taccgagcgg tacggaagtg agcagaatta
    15961 ataccagcgc cgcacccgcc agttccagcg tggcgggcag gaagttcagc atatcgtcca
    16021 gcaccgggcg ctgcgtggca aatgagatgc cgaaatcaag atgcagcgcc ttccacaacc
    16081 aggtgccgta ctgcacatac agcggctgat ccagtcccag catggtgcgg gtagaggcca
    16141 gcatctccgg cgtcggcggc aggttagaca gacgcaaata atcgagcgcc gggtcgccgg
    16201 taccgaggcg cagcatcaga aaaatgatca ccgaggcggc gagcaccatc gggatcagca
    16261 gcagaaagcg gcgtaatacg taacgcaaca ttaaggtttc accggtttaa tctgttcgaa
    16321 cggaatttcg gtggcgatcg gcgcgtaggg gatgttaccc agctccggtt ttgataccac
    16381 catcattgag atgtaactga taggcagata aaccgcctcg tcatgcagac gggtcagaat
    16441 gtcgcgatac agtgcctgac gttgtgcttc gtcatgggtc gccagcactt cgccgatctc
    16501 tttatcaatc agcggcttgt cggctaatcc ttgctgtgcc tggaagtcag cgtgcgacgg
    16561 tacgcgcatt gaactcagga aggcgtgtgg atcgtatggc gcgccccagg tgcggtggaa
    16621 aatcatgcca aaacgaccgt cgcgctgacg cgcatagata ctgctctctt cttcgccaat
    16681 cagcgagaca tctgcgccaa tctggcgcat atcagcctga atgatttccg ccatcgattt
    16741 gcttaacgca tcggtgccga tgaacgaaag ttcaatgtgc agcggctgac catttttctc
    16801 gcggatgtct ttgcccgcag gcagcgtcca accggctttt tccagcaacg ctttcgcttt
    16861 ctgcggatcg tactggcgcg gtttcaggcc gaggttggcg tagggcacag aaggggcaaa
    16921 cagggtgtcg gcgacctgct gggtgccata caacgcgtta tcaatcagcg attttttgtt
    16981 taccgcgtaa ttaagagctt cacgtactgc cagctcattg gtgggggctt tggcggtatt
    17041 aagcgccagc atcacggttt cgatcggctg tgacagttgg gtatggtaag ccgggttctg
    17101 gctaaagcgg gcgaaggtat cgagcggtaa taacccttcg tttccgtaca gcaggtcgat
    17161 atcgccagtt tcaaacgcca ccgcgcgggt agtcgggtct gggatgacgt taaaagtgat
    17221 cttcttaatt gccggctttt cgccccagta gttttcgtta cggacgaaga catcgtactg
    17281 attcagtttc gattcctgca aaacccacgg tccggtgcca atcggcgctt taatcccgtt
    17341 catggtttca tggtttttaa actgcgaggg ggcgataaag cggaaagggc gcggcagggc
    17401 cagttcttgc aggaaaggat agtaggcgct tttcagggtg atttgcagct ctgttttact
    17461 gagtgcttta acatcaacaa tctggtttgc cagctccagc caggcgtgac gttgacggtt
    17521 atcgagcact gcgcggaagt tttctgccgc cgcctctgca tcaaacggtt cgccgttaga
    17581 gaatttcacg tcatcacgca gggtgaaggt ccaggtttta ccatcttctg aatgagtcca
    17641 gctttttgcc agccacggga tcaccgaacc gtctgcctga tatttcacca atggttcata
    17701 aaccatgctc tgggcgaaca tctggttagg cgtgtaaagg tgcgggttta gtggcccgac
    17761 attcaccggc caggcggtgg tgatttcatc tggtgcagcg gcatggacga taaaagacgc
    17821 acaagccagc agcgcaaata gagtgcggcg gagtgtggag agcatggtaa ccccaatgga
    17881 ttaaaataga tggcggaaat aagtatgacg attttaagta ttcgtcatac tgattacctg
    17941 ttctggatca atagatgggc agtcgggtgg gcggatcagt taactgaatc gatccattgc
    18001 acactgtcgg cggtgagcgt aaagggagta ggggtgcaga tcgccaggct taaattttct
    18061 agctgacaat ggctgactga cagcgaggag tgataggtgc tgtctacgct gacgatttgc
    18121 caggcgctgc caccacgctg tttaacgatg gcttctttgc gcgtccagat gcgccagaac
    18181 atttccagtt gctgatcagg atgcacggcg tccatctcag catgttcccc gaggctgaac
    18241 acggcgttcg ccagccagcg ccagttggcg cgcgggcgaa tcacttcgat atcgcagccg
    18301 acttcgcctt catcactcaa cagcagggcg atatcgtcac cgctatggct taagttgaac
    18361 catagcggcg tttccggcgc aaatgcaggt ttgccttgtt cgccatagat gatctccggt
    18421 agcggggaaa gcgtgtgcga aagcaatgca cgccccgcca gccagcgttc gcgtcgtgga
    18481 ccttgcggtg cttgctcgcg taaacccggt ggcagtggag ctgcgcttaa ggtcgaaact
    18541 ttccccagaa ctatccgata catatcaggg ccaacgttta atgatgatgg atgtattaat
    18601 gccgccaaag gcaaagttgt tactctgcaa aaactcacaa tcaaccttgc gggcttcatg
    18661 catgatgtaa tccagcgcgc cacaattcgg gtctggttta ttcaggttta gcgtaggcgc
    18721 aaaccaacct tcgcgcatca tttgcagact catccaggct tcgagcgcgc cacaggcacc
    18781 aagcgtatga ccaaaataac ttttcagcga ggagagcggc acattatcac cgtaaatggt
    18841 tgctgtcgcc agactttctg ccatatcacc gcgatcggtc gccgtaccat gcgctgaaat
    18901 atagccgata tcttgtgcgc ttaacccggc gattttcagc gattgctcca tacaatactg
    18961 catggtttcg cgctgcggct gggtaatatg tgccgcgtcg cagttagtcg caaagccgac
    19021 gatttcaccg taaatggtcg cgccgcgcgc tttggcgtgc tccagttctt cgagaatgag
    19081 cgtacctgcg ccttcaccaa tcaccagccc gtcgcggttt tcatcgaacg gtgaaggtgt
    19141 ggttttcggc gcatcattgt gctggctggt ggcgaaaagc gtatcaaata ccgctgcttc
    19201 tgaaggacat aactcttcgg caccaccagc aaccatcacc gtttgataac cgtggcgaat
    19261 ggcttcccac gcataaccga ttgcctggct gccggaagta caggcgctgg aggttgggat
    19321 cacccgcccg cgcaggccga aaaagaggcc agtattaacg gcggtggtgt gcggcatcat
    19381 ctgcacatag gtggtgccag tgatgttatt ggtgtgcttt tccgtcagca tggtggcgaa
    19441 ctcgcttacc gggccggtac tgccggtcga tgaaccgtac gcaatgcccg tttcgccgct
    19501 ggttaatatc ggatcgccaa tcaaccctgc ctgctctaac gccagctcgc tggcacgggt
    19561 cgacatttgc gacacgcggc ccatcgcacg gatacgcttg cgggtgtagt gctccggtag
    19621 cgtgaaatca tcaacaggcg cgccaagcag ggtgtgcagc ccatcgtaga cctgccactc
    19681 cggcattttg cgcaccgcat tttcatatgc cagtaagcgt gcagaaacgt cctgccagtt
    19741 ttcaccaaag gcagtcacgc cgcccattcc tgtaatcact acgcgacgtg tcataacatc
    19801 cctccgttga tggaaataac ctggcgggtg acgtaacccg caatatcaga cattaaatag
    19861 ctggcaagcc cggcaacttc ttccgcctgg cccatgcgtt tcatggggat catcgacatt
    19921 gcctctttca gggcgctctc ttccatttcg atcatcccgg tatcaataag ccccggcgcg
    19981 atgcagttga cggtgatttt gcgcttcgcg agttcgatag ccagcgcttt tgttgcgccg
    20041 ataattccgg ctttggcagc gctgtaattc acctggccgc gattgcccat tacgccggaa
    20101 accgaggata gcgtgatgat gcgcccaccc tggcgtgcgc cgatcatcgg cataatgcac
    20161 ggctgaatga cgttgtagaa actgtcgagg ttggtgtgga tcaccgcatc ccagtcgtca
    20221 tcgcttaaag ccggaaaagc ggcatcgcgg gcgatcccgg cgttactgac cacgccgtac
    20281 caggcaccgt gttgcgcgat ttcgtgctcc agcacttccc gacactgttc gcggtttgcg
    20341 acgtcgaaac tgagcaaacg cccgttaccc ccattggcga caatggcatt cagcgtttcc
    20401 tgagcgcctg tggcgtcacg gtgatagtga acgccaatgt taaagccgtc tgccgccagt
    20461 tggcaggcga tggcgcgacc aatgcctttg ctggcaccag taaccagaac tgaacgactc
    20521 atcaggacgc tccttgttga aacagggtgg taagttcttc cgcggtcggc tggaaggtgt
    20581 taatgcggcc cgtcgcctcg ccgtcgttga tgctgcactc aaaactgccg aagcgatcgt
    20641 cttgcatcag cagtttgacg gtaatggtca gtgtctggcc tgcgggcaga atgccagtgg
    20701 tgcaaagcaa ttcacgcgcg cccagtatca tccctaactc aatactgttt ttgccctgct
    20761 ggtggcgatg ccagccagac cagacgccga cggtttgcgc catcagttca agggtaaacc
    20821 agccggggag attgccgtcc gggtcgagaa acggtgccag tacgccactg ggcgaaaccg
    20881 tcacgcggca caccgcgctg tcatcgctga cgctcaccac gtcttccagc agtaacatgg
    20941 gcgcgtcgtg gggcagataa gcgccggggg ataaatagtg gctcacgaaa ccctcccgag
    21001 cagaatgctg gcgttattgc cgccaaaggc gaaggaattg gacaggatca ccggacgtgc
    21061 cagcggctgc ggtttttcga taatgccaca cggtggcagc gtgggatcgc gcggcgacag
    21121 gctgaagtcc tgagcgggca gcggtaaatc acgctgtaaa atcaacatac taattgccgc
    21181 ctcggtaatg cccgctgtgc ccagcgtgtg tccggtcaaa tgtttggtgg agctgcacgg
    21241 tacgcgttcg ccaaacaggg cgttaaccac cattgattca atctgatcgt tgagttgtgt
    21301 ggcggtgccg tgcaggttga tatagccgac gtcatctggc gtaagctgcg catcggtcag
    21361 cgcctgatta atagcgcgga tcgctccttc gccctgcgga tgcggggctg aaatatggta
    21421 agcgtcgctc gattcaccaa cgcccagcaa cgcgatgggc tgcggttccc gggtcagcag
    21481 cattagcccc gccccttcac caatggtgat acctgcgcgg tcacgaccaa acggctgaca
    21541 taaggttggc gacagcgatt cgaggctgtg aaaaccgtta atcggcatcc ggctcagggt
    21601 gtctgcgcca cccacaatgg cggcatcgac cagccccgct tcgataagcc tgcgcccgct
    21661 gattatcgct ctggcgctgg aagagcaggc ggttgaaagg gtataggccg ggccgtcgag
    21721 cgccagccag tggctgagaa aacgcgacgg atcgccgagt tcctgttgcg cgtagtgcca
    21781 tgtggtgctc ggctgcccgt gggtgcgcag gttaacgtgc gtgtcgcctt catgcaggcc
    21841 ggaggtgctg gtaccaagca caatcgcaat gcgctcgcgg ccatatttcg caatggcgtc
    21901 atcaacctgc ggctggatct gcgccagcgc cgccagcagg atctgattgt tgcgcgagcg
    21961 gtgcgcagcg aatttttccg ggataagcgg aagctcgcca tccacgccag ccaggaccgc
    22021 ctgcggatgc ccctgcaacc agcctgcgcg cgggcgcatg ccgggagcaa caccgcgagt
    22081 caggttggtt gcgatttcgt caaggttatt tcccagggcg ttgatcatgc cgacggcgga
    22141 aatataaatc atatcagtca cctaagtatt gaatggtgat gtggtattta aagacatgct
    22201 gctcaatgct gattggcacg cgtttgccct tgcgattcag ataggtgatt tccgtgacca
    22261 gtttgccgct ggcgttacgc agctcgcgtt tgtcaccgtt gtcgcgaagt gtccatcctg
    22321 cgggaagttg cggttgccag gcgctaatcg gccagtggct gagcatcacg tcagccagta
    22381 cctgacttgc gggcggtaac tgtgggacga cgatggattg ctcggcgcgt agcccttgcg
    22441 catcgtaggt caccagaaac aggcgaatgc cgaccgacga cagcccggca agggtgattt
    22501 tctgatcgtc ggcgttaagc atcactaaca gcgactgggt tttgccgttg aagctgccgg
    22561 tcaacagttg ctgcgaattg actgcgggtg aaatccctgg tgccggcagc gtgatgcgcg
    22621 tgccaggttg cagccacgcc tgcgggcggc cttcctgttc tggttgcgag tggctacagc
    22681 cggtgaggat aagcgtagcg gtcagggcga acgctcgcca gaatgtggat ttgatcattt
    22741 ttttgttctc tttttatcgg gcatagccag cggcgaaagc aggaaggcgg tgaaaatacc
    22801 gctcaccagc acaatgccaa aactgctgat ggcctgggtg gcgctgaata ccagcatacc
    22861 cagcgtcagc aaggtggtga gcattgccag cgcgatcgcc agtagcgaag ttaacggtgt
    22921 accgcgcgga ttactgaaaa acagcgtgta gttgataccg atgccaagca ccagcaccag
    22981 cgccagcagc gaaaagagat tcaccgcctg cccgctcatc gccagcaccg ccagaccaca
    23041 gcccagcgaa agcaccgaag gcaccaggct gataagccct ttgcgccagc cgagacgggc
    23101 cactgcgccg caggcaatca ctgccagcgc caccagcaac aagccggtta agacgtagcg
    23161 gtaaagtgcg aacaattcat caaaggtgct tttgcgatca acccaggcaa tgccgcaagg
    23221 gtaatatgtg gcgatttcct gcatcaacgc gctactttta accccttcaa ccggcaccag
    23281 tacgccgctt tcgccgtttt ccagcgtcag ccacagcaga cgccagcctt cactggcggg
    23341 gcttgccagc cattcatcaa cgttcactgg catggcgttg agatccgggt tcactgccgt
    23401 cagcccggca ttttgcagcg cttttgttac tgtcggggcc gccgttttca gcagttgtaa
    23461 atcttcctcc tgccgcgcaa gggagttcag cggaatggtg cggtagttgc tgataagccc
    23521 ctcttttttc gcatactcaa gtgaggcggt atatttctcc agtcgccgca atgtttgctg
    23581 tggcgaatcg ccgtaaacca caaaccattt ttgatcgacg ctctgcccgg tcagggcggt
    23641 aatggctttt tcctgcgcca gaatatgctg cggtagcgcc tgtaactgcg agatatcgtc
    23701 atcgacgcgt agcattgaca tccccgccag cgaaaacagc gccagcgcga cgggcagacc
    23761 cagcgacagt tttttattac gccgccacgc tgccagccag cgtagcatca gcgccatcgc
    23821 cggaaccgga cgcaccggca ggccacggca cagccacgga tgccagaaca ggacggtcag
    23881 acaggaggcg ctcaacccga cggcggcaaa aatcgccatc tggcgaatgc cggggaaggg
    23941 ggcgagcatc ataatcagat acgccgccac ggtggtgagc agcgccagta gcaaggcatt
    24001 gcgtactttc gccaggcttt gccacggcga aacgtcgttg ccgtgaacca tccgctcggt
    24061 gagataatag agcgtgtagt cagcggaaat gccgataacg ctcatgctca tcaccagcgt
    24121 catcaggtgt aattcaccga aaatcaataa agtggcgacc gttcccgcca gcgcgccgat
    24181 gccgatggaa atcacgcaaa gcagcaacgg gcgtaaagag cggaacaccg ccacaatcag
    24241 caatatcacc cccagcagcg tagccacgcc cagggtggag atatcctgct tcgcctgttg
    24301 gctggcgtaa tcgctgtaaa acaccgtgcc gcgcgagagc aactgtgcct gcgggtaacg
    24361 cgttttcaga tccttttcca gcgtattcag ggtcgtgatc agctggtggg tttgctgcat
    24421 atcaaacgac gatcccgcca gttcgccgtg cagcagatac cagtagttgc cctggggatc
    24481 ctgcgtcacc agccaaccgt ccatcagccg caaacgctgg ccgtttttcg ccattgccag
    24541 ctgcgagccg cgcattaaca tcagcggatc gttttgcagc tccttgccgc ttacgccgga
    24601 gaatgcggaa taaagctggg agaggatcca ctgtgcctgc gcttcgccgc cgttttgcag
    24661 gcgggcgcgg gtgttggggt caatcaggcc gttgcgatgc tgccagaaaa acgctcccca
    24721 cgcctgctgg ctggcggcat ccattggccc tttaacgtcg ccgagcgcag cggatttttg
    24781 cagcagcgtc agccactcct gagcgaccaa aggattagcc tctttaccgg ggctgaccag
    24841 ccacaccagt tggcggtcaa gacgctgcat aaagccgtca ttcagcgccg gaggaatatc
    24901 gcccatcgtc tgtttgggta acatagccag cacgctactg ttcagccgtg attgcggcag
    24961 caggatcagc aacgccaccg ccatgaccag gcagactagc ccccataaca gcgcggggcg
    25021 tttactgggc ggcaaaacgt tggcgttcgt catcggtcag ttgcgctggc gtcagttgat
    25081 gttgggtaag agcaatatcg gtgcgatcgc cctgtttatc attaagttga atgctctcca
    25141 gataggtttt cccggcgaga tcgatggtgt tgaaaatttt atccagcggc gtggtggtcg
    25201 gcgtcaggcg cagcgtccag cggccttcgc ctttgtcagc aaattcgacg cggaagtttt
    25261 gttccagcac tttgcgatcg gcctggaaca acgcgcgcag caggtggtta aactggaaca
    25321 tctgcgggtt gttttctgcc gtgatgattt gcggcggctg accgttgatc acctgcacca
    25381 tgcgtttatc gtccagtaat agttgcatgg ggaacggtga ggtttgatcc cacaacagcc
    25441 cctggtcgcg ggcgatcaac atctggccct gagatcgcag cggctgcggc agatctttaa
    25501 tcgtccgggt ttgatcgaaa tgggcgcgga tcaccggttg ttcggtaaag cgttgctgaa
    25561 gatcgtccag ggtcagggcg ctcacaaacg ggctaatcag cagcgccagc agcggtaaaa
    25621 atttcatggt ttgactccca tgcgttcaaa cagaatatcg gggctgacaa agcacagttc
    25681 gcgactttgc tcgtcgaccg cgacctgaat ggtgtatccg gtggtggcgc gtttgcccgt
    25741 ttcggcatcg aaaatctgat agccaatccg cagacggttt tcgaactctt cgatatgcgc
    25801 acgcacgcgg atgcgttgct caaaggtgag ggcgtggcga tatttcaccc gcgcatcgac
    25861 cactggccac aaataaccgg actctttcat ttgccgatag ccgtaattga actggttgag
    25921 cagcgcctcg cgagccactt caaagtagcg aaaatagtta ccgtgccagg ccacgcccat
    25981 catatcgacg tcgtgaaagg gaatggtcag ctcgacttct gcggtaaatc ggggatcgtt
    26041 cagcaccctt tactccttgt cctgaatttc cggcagttgc cagaaatcga aaaaattaaa
    26101 ccagtcgaga ggcgactgga gcgcgtaatg ttccagacgc gcggcgtaat gatcgatagc
    26161 gttttgcagc gcctgttggc gttcgccgcg cggcagctgc agtgggtcgg caaagctttc
    26221 gcagtgaata tgcagcttgc cgtgctggcg cagggcgaaa atcagattca ccgggcagcg
    26281 caaaatagag gcgagaataa acggcccctg tgggaaaggc gcaggctggc ccataaacga
    26341 actccagcag acgcgccagt cgccgccgcg ttgcgggttg acggcgatgc ggtcaccgac
    26401 aatcgccacc cattcgccgt tatccagctt ctctttcagc aggatggcgg tttctgggcc
    26461 gatatctgtt accggcatca ggttaatgcc tgcctgagga gccatctcct gcattatctg
    26521 tttaaagcgt tgggcgtttt cgctaaacac cagcgcgtta atggttttgt agccctgaat
    26581 ttttgccagc gcccggcaca cttccacatc gccaagatgc gaggccagca gcaatttgcc
    26641 gcgcggatcg cggacgtcaa gcgctgcttc tgcgcctggc gcaaacagca catcacgccc
    26701 tggttgtagc tcgccgcgcc agctggcgat tttgtcgagc atggcattac cgaaacgtag
    26761 aaagtgctga tagctgttga gttttgccgc ctgtggttga tgctgtcgta cacgggcgag
    26821 ccagtcttgc gacgctttgc gcgcacgaga agcagtgagc cagtagacgc ccaccaccgg
    26881 gtaaagcagc gcgctaaacg ccgttcttcc cagcagacgc cagaccagca gcatcaggcg
    26941 cattccccat aatcctttca cttcactctg ccgcgcccag tggcaggaag agcggcgcat
    27001 cagcagtgaa ggaatacgcg gcaacatgcc gaaaaacaga cgcgtgtgca tgagcgagat
    27061 gcggacgtta tctttcaggg catcaaaatg cgaaagcccg tccagtggat aggtgacgcg
    27121 ggtcggcacg aaatagctgg tatttccctg ccagtagagg cgcaccatca cttcggtgtc
    27181 gaaatccatc cgcttgccga tggtggcgtg ttttgccagt tgcagcgttg gcgcaaccgg
    27241 ataaacgcga aaaccgcaca tgctgtcttt cagttgcagg gagagcgttt cgatccatac
    27301 ccagacgtgg gtgacccagc gcccgtaaag ccgtgagcgg gggatggagt catcgtaaat
    27361 tggctggccg gagatcagtg ccgcaggttg ttgttcagcg agagccagca gtttagggat
    27421 atcttcaatc gcgtgctgac cgtcagcatc cacctgcacc gcatggctga accctgcgtc
    27481 tgccgctgcc tgtaagccac gcatcaccgc cgcgcctttg cctgcgtttt ctgccaggcg
    27541 aattaaggtc acgccaggct gttcggcaag cagactgtcc agttgctgtt gtgtggtggc
    27601 atcgctgccg tcatccacca caatacaggg cagattaaat ggcttaagac gcgccagcac
    27661 gcccggcatc atcgcgccgt ggttgtagca gggtatcaac acgcagggag aaaagtttac
    27721 cgacataggc ggatcttccc gctactggcg gtgtggcggg cgtcaccgtc gtgacgctga
    27781 tagctaaagc tcagaatctg gcgttcttcc tgccagttaa gcgcgagcgt gacgcggttt
    27841 tccggcagca acggagactg gaattttatg ttctgaatgg agtgaaaacg ccagccaggc
    27901 gcgagcagat tgagcgcgta gctcatcgcc cagtcgattt gcgcaactcc tgggagtaat
    27961 ggttgcacgg caaaatgacc gcgaaaccag aacagatccg ccctgagatg cagaacaatt
    28021 tcaaggtgat tcgtttgtgc ctgatggcgc tcgatttcat ggggcttcat gaaataactc
    28081 ctgtaattgc gcatagacac gcttgttcat actgtttact ggcatttcat caataacgcg
    28141 ccagtaacgc ggaatagcaa ctggctccag cgtcgggcgc agtaaacgcc gccaggtcat
    28201 ttcctggcta tgcccgccgc cgttctgcca ttgcagatga gcttcgtcat ccagcaccag
    28261 caaaacgcct atgctctgac gcccgccgcg cgtaacggga actgccgccg cctcgcgaat
    28321 gccgtccagc gccagtagac gttgttctac ttcctggagt gaaatacgtt tttcttcaat
    28381 cttaacgatg cgcccacgac gccccatcag atggaactga ccatcttcgc taaactgtaa
    28441 gatatcgtcg agtaacatgc cgttatcttc cgctatcagc ggtgaaaaga ggcgaaaggc
    28501 atcgccttcg gcctagaact gcatacctgg gaggggtagc cagcggcgtt gctcttgttc
    28561 gcggtagcgc caggcgatga cgccagtttc cgtgctgccg tagatttcat caggccagac
    28621 gcgcagccag ctcgcggtat gttgtacatc ctgccacggc agctcaccgc cagcagagag
    28681 aagcacctga acgggtggcg gagaaagctg ggtatccagg cgcttcaaaa atgccgggct
    28741 gctgatgaag atatagcgat gctgatgact caacgcggca aactgttcga cataccagag
    28801 catggcggcg tgcagcggta atcccaacgc catgggcagg aaaacgcgaa aagtcaggcc
    28861 gtacaaatgc tgcggcaata cggaaccgac gacacgacaa cccgccagac gtgcgcctaa
    28921 tcgttcagcc agcagttctg cttcacgatc cagcaaatga acaggtttaa tcgcccgttt
    28981 cggctgtccc gtggaaccgg aggtaaacag ctcgataaaa gcctctggcg ctatagcggc
    29041 aaaggtgaag gattgtgtcg cgatttgtgg agaacttgct accaacagca atgaaccttg
    29101 ccagttaagt tcgctgtcgc taagcacacc atcaaaaagt tcacgttgct cgttaagctg
    29161 gataacacgg ttatgccccg gaagcaccgg cgtttttccg gcgtgtagcg tggcgagcag
    29221 ggcaacgata aacagatagc tgttctcaaa gcacagcgcc cagcgttcac ccggctgttg
    29281 ctgcaagcgg cagataagtt gcgcgacatc gtgacgcaaa tcgcccagcg tccaggtact
    29341 ttcgttcagc cacgcaatgg gtgtgtcatc agggcgtggc gcagtcagcc agcgtgcaag
    29401 cggtaaggtc tgcttcatgc gcgatctctt ttcatcacct gacggcgcag cagccattca
    29461 ccagccatta acgtccccat caacaaataa gcaatcatgc cgttccaggc ggtccataac
    29521 gacatatcgg cataaagcgc ggtaaagagg gcaattccac cgttaatgat gaaaaagccg
    29581 caccagattt gcgtgacgtg gcgggtatag cgcacgccct tttccgggag gtctggctcc
    29641 tgcaaccgcg ccagccgctc aactatcggc attgccgacc acagcgaacc gccaaaaacc
    29701 gccagcatga ccgcgttaac cactaccggg taaaacagca gtagctggtg cgttttcaac
    29761 ataaagctgg cgacacataa tgcgatgccc acgacagcga cgagttgcgt gacggcttgt
    29821 aaaggccccg cctggcgacg ggtctggcgc agacgcaaca aaagcagcaa cgccattagc
    29881 ggtaacagcc agtgcaggcc gttatgcgct aagccaaacc aaatcacaaa cggccaggcg
    29941 agcagcaata atccagtcag cagcttaatc atcggtagcg agcgaatgcc cgacatcaga
    30001 tcaggcttct tgtagcaggc gttctacagc ctcgacgaca tcctggactg tacgcactgc
    30061 tttaaactct tccggcttga ttttcttacc ggttttcttt tgcaggtgca caatcatgtc
    30121 aacggcgtcg atgctgtcca gttccagatc ttcgtacagg cgcgtttcag gtttgatgtc
    30181 ctgcggatcg atttcaaaca gcttaaccag cagggctgag acttcctgat agacggtttg
    30241 ttgttctgtc atgatggctt tcctcaggca cgttgcgcag caatgaaaga ggccagagtg
    30301 gcgacggaga aaaagtgctg acgcatctct tcgctttccg cagagagcac gatgccgtac
    30361 tcatttttta ctgccagtcc cagttccagg gcatcgatag agtccagccc taaaccgtcg
    30421 ccaaacagcg gcgcgtcggt atcgatatca tcaggcgtca actcgtccag attgagggtg
    30481 ctgatgatga ggtttttaat ttccagataa agcgcttgca ttatttattc ctgagagagg
    30541 taaacaacct tgttgtaatt gaagcaaaag atgccggttt aactgccttg ctgccagcgc
    30601 cggttcttgt gaagttgcat cgtaaaattt attgatttca acccgaccat ggactgcaac
    30661 cgtaaaaaac ggccggtcag gtggaacgtc ataccattta ctttgtttat ccagcagatg
    30721 ctcgctacag ccgattgtca cgatgcgtaa atcgctggcg cagcgcacgg caatattcgc
    30781 cgccccgcgc tgcagcgtca ttttttcacc gggcttcgtt cgtgtgcctt cggggaaaat
    30841 caggatagtg tcaccctgtg ccagtcgctg ttggcagcgg ggtaacagag tttcagcttc
    30901 gctgttgatt aaataatctg tcgcccgcac gacgccgcca agaaaaggat tttttaacag
    30961 cgcgcttttc accaggcagt cagtttctgg catgactgac gccagcagca cgtaatcgat
    31021 cagcgtggga tgattggcta cgacgaggca accgcgttcc tgatgtaaga tttcagcgcc
    31081 atcaatacga taatccagta cgccaagccc tttcgcgaca gttaaaaaca ggcgaaaact
    31141 ggcggcaatg ctgcgacgcg ccaaacgacg gcggcgggaa gtatctcaca ccaaaactaa
    31201 caatatgtta aaccacacga cagaaagcag caggccgccg agaccaaaca gggcaaaaca
    31261 taacccggtc atgaccagtc gccaggtcca ctccagacgt gacatcagct tactcatctg
    31321 tggctccagc gccattgcac gcgctcgcca ggcagtgaaa atgtgtctac attttgtaaa
    31381 tagtgctgta aaaacaacat gctttgcggc agtgttgttt cattgcccgc aatggctgac
    31441 tgcgtttcac attgccagtc atcgccagct tcaatcacca gcgcgacggc gtacggccag
    31501 gtcggcattt ctgatggcag ttgtgggtga taaaactcgg gcaggaaacc gtcaaaatcg
    31561 accatcagca cgcgctggta tcccgcttgt agcaggctaa gcacttcaca caaaccttgc
    31621 tggaaactat cgcgaccagc cgaaagcgat gacgaaacgc tcggctgttt ggccgcaatg
    31681 gtcaggttac ccacggaaga gttgtgtacg gagagcgcga agtccgtcgg agaaagcgcc
    31741 tgttcggttg ccagtgcatg aacaatgcgg taattgcgct ccagttcgcc atgacgactg
    31801 gtatacaaaa cggcgtctgg ttgatggcgg cgtagcattg ccagaccgca ttcaacagcc
    31861 agtttgctcc cggagctgag gcggcgggcg gtcatcattg gcagttcact taattttgcc
    31921 aacggtgctg tcgggtcaat tgcctgaggc tgacgtgacc actcctgcca ttgcagcgtt
    31981 tcactaagtc caggcgctct tgcctgccag tcgagaatgt ttaatgcaaa tttcatcaac
    32041 gcgcgcccgc ggtaaaccaa aattattaag tgacgattat ttcttttggc atatcaaaag
    32101 tgtatgccca acgcctaaat tatcgtggcg ctctgctacc tggaatcctg ctttattcag
    32161 atagtcgtaa aatttctctg cactatagaa acggctgtta ccgtttgcca tgcaggtaaa
    32221 ataaagtgag gacgcgttca ggctaaacga tgctgcttca aaccgctgcg catcccagaa
    32281 caactccatg atgcaaactt ttgcgccagg cttcattacg ctggcaattt tgctgagtat
    32341 gctaattatt tgttcgggtg aaaaacagtc taaaaattgg ctcatccacc agatatcagc
    32401 ctcgcccggt aatggggcat cgcttagcat atccaccgca tggaaatcta tgcgatcaga
    32461 aaaacctgca ttcgcgatat tttctttcgc tagtacgatt tgttgtggca gatctaataa
    32521 cgtaacggcg atgttttcat tgtatttgca gcaacgcaaa gcccacttac ccgtattgcc
    32581 gcctacatcg tacaatgttg tggggttgtt ggcgaataca taaggcagtg cggcgttaaa
    32641 tgcgccatct gaatagtaat gatcaaacgc aaaccagctt tcacgagcgg cgtctggtaa
    32701 ttgcgaaagt gccggatata tcgtcgacca gtcgccaaat acttttaacc cggaaggttt
    32761 tccttcattg agtgaatcgg cgaggaagaa gagcccctgg taacagacat cctgggtgaa
    32821 atccatattc acgcgagtca tagtgtcatg caggaggtaa tgaccgattt tcgccaggta
    32881 ataaattccc tctttacagg taataattcg cccgcttaag cccatatcta acagaacgct
    32941 ggtagcatat tcgttgatat ggctatgttc ggtgatatca gccagcgttg cgccatgttt
    33001 gccttgccga tccagatatg aaagtacacc cgcattacgt aggcagagtg ctgtttgaaa
    33061 aagcataggg gcaaaggcaa tgcgttgagc ttcggtaatc gcatccagtg cgctaagaga
    33121 atcctgttcg tacatgttgg gcacaacctg ttaagaggga attaaattat tatcggaccg
    33181 caacggcccg actttaggaa gaattataaa tctgcgcccg ctgacaaatt cagttgaata
    33241 tctttgtcga gattgcggac ccagcgattg tagtttttat gaatttgtcc gcctgacgct
    33301 tgtaggttct ggctattttc ataattgata tcgtagctgg atgccgtgta gttaatgcgt
    33361 atttccgctg aatgatcgcg tgactgttga cgtgctttaa ttatgccttg tccggcttcg
    33421 gacataatcc atttgcgttc aacgccagct ttcagaatcg cttttttcac ttgttcctgg
    33481 gtatgaccag cgctgacggt tgagtgaacc tgatcaattg gcgcagtacg cgcgcaacct
    33541 gctaaagcac ccacaacagc aatagccgca aaccatttca tgtaatgtct cattagaacc
    33601 ccattaaagt attgttttta cgagtaaagc aagttttaac tgtaattgct ggcatcacta
    33661 ttctttattt agagaaaaaa ttacttgtga gataagtcag taagtgcaca tcagagtaaa
    33721 aatctctgaa atgactaaat tac
//
LOCUS       DBFIFS010000028        33714 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.028, whole genome shotgun sequence.
ACCESSION   DBFIFS010000028 DBFIFS010000000
VERSION     DBFIFS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.028"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..558
                     /gene="yraH"
                     /locus_tag="N7A07_001251"
     CDS             <1..558
                     /gene="yraH"
                     /locus_tag="N7A07_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2475947.1"
                     /translation="AGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKKIEVALGH
                     YNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPN
                     LAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGIVNVDLIAYY
                     VSTVEASEITPGEADAVVNVTLDYR"
     gene            638..1333
                     /gene="yraI"
                     /locus_tag="N7A07_001252"
     CDS             638..1333
                     /gene="yraI"
                     /locus_tag="N7A07_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2475948.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1362..3878
                     /gene="yraJ"
                     /locus_tag="N7A07_001253"
     CDS             1362..3878
                     /gene="yraJ"
                     /locus_tag="N7A07_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2475949.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3889..4622
                     /locus_tag="N7A07_001254"
                     /pseudo
     CDS             3889..4622
                     /locus_tag="N7A07_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4665..5525)
                     /gene="rsmI"
                     /locus_tag="N7A07_001255"
     CDS             complement(4665..5525)
                     /gene="rsmI"
                     /locus_tag="N7A07_001255"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU2475950.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5590..7626
                     /gene="lpoA"
                     /locus_tag="N7A07_001256"
     CDS             5590..7626
                     /gene="lpoA"
                     /locus_tag="N7A07_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU2475951.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7584..7979
                     /gene="yraN"
                     /locus_tag="N7A07_001257"
     CDS             7584..7979
                     /gene="yraN"
                     /locus_tag="N7A07_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU2475952.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            7999..8589
                     /gene="diaA"
                     /locus_tag="N7A07_001258"
     CDS             7999..8589
                     /gene="diaA"
                     /locus_tag="N7A07_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU2475953.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8599..9174
                     /gene="dolP"
                     /locus_tag="N7A07_001259"
     CDS             8599..9174
                     /gene="dolP"
                     /locus_tag="N7A07_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU2475954.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9288..10328)
                     /gene="yraQ"
                     /locus_tag="N7A07_001260"
     CDS             complement(9288..10328)
                     /gene="yraQ"
                     /locus_tag="N7A07_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU2475955.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10401..11036)
                     /gene="yraR"
                     /locus_tag="N7A07_001261"
     CDS             complement(10401..11036)
                     /gene="yraR"
                     /locus_tag="N7A07_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU2475956.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11164..11682
                     /gene="yhbO"
                     /locus_tag="N7A07_001262"
     CDS             11164..11682
                     /gene="yhbO"
                     /locus_tag="N7A07_001262"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU2475957.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11662..12105)
                     /gene="yhbP"
                     /locus_tag="N7A07_001263"
     CDS             complement(11662..12105)
                     /gene="yhbP"
                     /locus_tag="N7A07_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU2475958.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMRGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12156..12458
                     /gene="yhbQ"
                     /locus_tag="N7A07_001264"
     CDS             12156..12458
                     /gene="yhbQ"
                     /locus_tag="N7A07_001264"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU2475959.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12445..12948)
                     /gene="yhbS"
                     /locus_tag="N7A07_001265"
     CDS             complement(12445..12948)
                     /gene="yhbS"
                     /locus_tag="N7A07_001265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2475960.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(12942..13466)
                     /gene="ubiT"
                     /locus_tag="N7A07_001266"
     CDS             complement(12942..13466)
                     /gene="ubiT"
                     /locus_tag="N7A07_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU2475961.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13675..14670
                     /gene="ubiU"
                     /locus_tag="N7A07_001267"
     CDS             13675..14670
                     /gene="ubiU"
                     /locus_tag="N7A07_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU2475962.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14679..15557
                     /gene="ubiV"
                     /locus_tag="N7A07_001268"
     CDS             14679..15557
                     /gene="ubiV"
                     /locus_tag="N7A07_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU2475963.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15638..16645
                     /gene="yhbW"
                     /locus_tag="N7A07_001269"
     CDS             15638..16645
                     /gene="yhbW"
                     /locus_tag="N7A07_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGU2475964.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16762..18006)
                     /gene="mtr"
                     /locus_tag="N7A07_001270"
     CDS             complement(16762..18006)
                     /gene="mtr"
                     /locus_tag="N7A07_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU2475965.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18160..20049)
                     /gene="deaD"
                     /locus_tag="N7A07_001271"
     CDS             complement(18160..20049)
                     /gene="deaD"
                     /locus_tag="N7A07_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU2475966.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20042..20122)
                     /gene="yrbN"
                     /locus_tag="N7A07_001272"
     CDS             complement(20042..20122)
                     /gene="yrbN"
                     /locus_tag="N7A07_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU2475967.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20229..21113)
                     /gene="nlpI"
                     /locus_tag="N7A07_001273"
     CDS             complement(20229..21113)
                     /gene="nlpI"
                     /locus_tag="N7A07_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU2475968.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21222..23357)
                     /gene="pnp"
                     /locus_tag="N7A07_001274"
     CDS             complement(21222..23357)
                     /gene="pnp"
                     /locus_tag="N7A07_001274"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU2475969.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23604..23873)
                     /gene="rpsO"
                     /locus_tag="N7A07_001275"
     CDS             complement(23604..23873)
                     /gene="rpsO"
                     /locus_tag="N7A07_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU2475970.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(24022..24966)
                     /gene="truB"
                     /locus_tag="N7A07_001276"
     CDS             complement(24022..24966)
                     /gene="truB"
                     /locus_tag="N7A07_001276"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU2475971.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDVDGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(24966..25367)
                     /gene="rbfA"
                     /locus_tag="N7A07_001277"
     CDS             complement(24966..25367)
                     /gene="rbfA"
                     /locus_tag="N7A07_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU2475972.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25532..28204)
                     /gene="infB"
                     /locus_tag="N7A07_001278"
     CDS             complement(25532..28204)
                     /gene="infB"
                     /locus_tag="N7A07_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU2475973.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28229..29716)
                     /gene="nusA"
                     /locus_tag="N7A07_001279"
     CDS             complement(28229..29716)
                     /gene="nusA"
                     /locus_tag="N7A07_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU2475974.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQVQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29744..30196)
                     /gene="rimP"
                     /locus_tag="N7A07_001280"
     CDS             complement(29744..30196)
                     /gene="rimP"
                     /locus_tag="N7A07_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU2475975.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30403..30479)
                     /locus_tag="N7A07_001281"
     tRNA            complement(30403..30479)
                     /locus_tag="N7A07_001281"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30443..30445),aa:Met,seq:cat)
     gene            30827..32170
                     /gene="argG"
                     /locus_tag="N7A07_001282"
     CDS             30827..32170
                     /gene="argG"
                     /locus_tag="N7A07_001282"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU2475976.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32178..>33714)
                     /gene="yhbX"
                     /locus_tag="N7A07_001283"
     CDS             complement(32178..>33714)
                     /gene="yhbX"
                     /locus_tag="N7A07_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU2475977.1"
                     /translation="ASSGAHMVQRLLFFVLTILVVKRISSLPLRLLVAAPFVLLTAAD
                     MSISLYSWCTFGTTFNDGFAISVLQSDPDEVAKMLGMYSPYLCAFAFLSLLFLAVIIK
                     YDVSLPTKKVTGILLLIVISGSLFSACQFAYKDAKNKNAFSPYILASRFATYTPFFNL
                     NYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDTYVLIVGESVRVDNMSLYGYTRSTT
                     PQVEAQRKQIKLFNQAISGAPYTALSVPLSLTADSVLSHDIHNYPDNIINMANQAGFQ
                     TFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFDELLLPHLSQALQQNTQQKKLIVLH
                     LNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIHYTDSLLGQVFELLKDRRASVMYFA
                     DHGLERDPTKKNVYFHGGREASQQAYHVPMFIWYSPVLGDGVDRTTENNIFSTAYNNY
                     LINAWMGVTKPEQSQTLEEVIVHYKGDSLVVDANHDVFDYVMLRKEFTEDKQGNPTPE
                     GQG"
BASE COUNT         8300 a         9055 c         8359 g         8000 t
ORIGIN      
        1 gcgggcttac ttgcgctttt cgcaggtaat gccgctgcaa ccgatggtga aatagttttt
       61 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
      121 gctcttggtc actataatgc cgaacagttt cgttctgtag gcgatcgtag cccaaaaatt
      181 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      241 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggtaccgt ccctaacttc
      301 cctaacttgg caaaagtggg ctcttttgct ggcacagcag caacaggtgt gggtatccgt
      361 attgacgatg cggaaagcgg aaaccttatg ccactaaatg ctatgggcaa tgataacaca
      421 gtttatcaga tccctgcgga ctccgctgga attgtcaatg ttgacctcat cgcttactac
      481 gtatcaaccg ttgaagcatc cgaaatcacc ccaggggaag cagacgctgt cgttaacgta
      541 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      601 cttcctattt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcgat
      661 gatatttacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      721 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      781 caaaggcgca gaaacacctt atttactgca atcatgggta gataatatag atggtacaag
      841 ccgtgcccca tttattataa ccccaccgct aattcgtctt gaagctggcg atgattcatc
      901 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      961 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
     1021 gctggtattt aaaacacgga tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1081 aagcgatgcg tggaaatcac tggaatttaa acgtagtgat cattcactca atatatataa
     1141 cccaactgaa tattacgtcg tatttgccgg actagcagtc gataaagccg atctcacaag
     1201 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1261 aaagaacgtg aaatgggctg cgatcaatga ttatggcggc agttccggga cagaaactcg
     1321 tccactgcaa taacaaatat aaaaacacag gtcatcaggg aatgccacaa cgacaccacc
     1381 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgctgt ttgccgatgg
     1441 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1501 ccattatgct ggaaaccaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1561 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1621 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1681 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1741 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1801 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1861 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1921 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1981 taaatatgca aaatggtgcc aattttggcc cctggcgatt acgtaactat tctacgtgga
     2041 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2101 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2161 gctacaactt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2221 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2281 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2341 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacgc
     2401 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2461 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2521 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2581 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2641 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2701 ttcatggcta tcaatggcgt acgcagtaca tcaaagatat cccggaaacc aacaccaata
     2761 tcgctgtcag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2821 cccgcaattg ggactataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2881 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2941 acgagaaaaa caggaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     3001 gcctgaatta tcaatacagc cgctacactg atcaaaataa tgaccgcgca ctctctttga
     3061 atctcagtat tccgttagaa cgctggttac cgcgtagccg ggtttcctat cagatgacca
     3121 gccagaaaga tcgcccaacc caacatgaaa tgcgtcttga tggctcactg ctggatgatg
     3181 gtcgcctgag ctatagtctg gaacaaagtc tggatgacga taacaaccat aacagtagcg
     3241 tgaacgccag ttaccgttca ccttatggaa ccttcagtgc cggatacagt tacggtaatg
     3301 acagtagcca atacaattac ggcgttaccg gcggcgtggt tatccatcct catggtgtga
     3361 cactctcgca atatctgggc aacgcttttg cgcttattga tgctaacggg gcatctggcg
     3421 tgaggataca aaactatccg gggattgcta ctgatccctt tggctatgca gtggttcctt
     3481 atctcacaac ttatcaggaa aaccgtctct cggtagatac tacgcagctg cccgataacg
     3541 tcgatcttga acaaacaaca cagtttgtgg tgcccaacag aggtgcaatg gtagcggcgc
     3601 gtttcaacgc caatatcggt tatcgcgtac ttgttacagt cagcgatcgc aacggtaaac
     3661 cgttgccctt tggcgctctt gccagcaacg atgatacggg gcaacaaagt atcgtcgatg
     3721 agggcggcat actatatctc tctgggatat cgagtaaatc acaaagctgg actgtacgct
     3781 ggggaaatca ggcagatcaa caatgtcagt ttgcttttag tacaccggat tcagaaccaa
     3841 caacctctgt attacaaggc acagcgcagt gccattaagg ataaaaaaat gaaaagagcg
     3901 cctcttataa caggactttt gttgatatcc acatcctgcg cttatgcctc ctcagaaggg
     3961 tgtggagctg acagcactag cggtgcgaca aattacagca gtgtggttga tgatgttacg
     4021 gtgaaccaga cagataacgt gacaggacgg gagtttacct ctgcaacgct aagtagcact
     4081 aactggcaat acgcctgttc ctgctctgcg ggtaaggcag ttaaacttgt ctatatggtc
     4141 agccccgtac ttaccaccac tggacatcag acaggatatt acaaactcaa tgacagcctg
     4201 gatattaaaa ccatgttatt aaggttaatt ttggattcat caatggtcag aagtttacca
     4261 cccgcaatgc catgccagac ggttttactc cagtagactt tgatatcact tatgactgtg
     4321 gtgatacttc aaagattaaa aactcgttgc aaatgcgcat cgacggtaca actggggtag
     4381 tagaccagta caacctggtc gccagacgaa gaagttcaga caatgcgccc gatgtcggta
     4441 ttcgtattga aaatctcggc ggcggagttg caaatattcc ttttcagaac ggtatccttc
     4501 ccgttgatcc ttccgggcat ggcaccgtca atatgcgcgc ctggccagtt aatctggtcg
     4561 gtggtgagct ggaaacagga aaatttcagg gcacagccac cattaccgtc atcgtgcggt
     4621 aaaagaagac gttgcgtttt gattaacgca acgcttcggc actcttaccc ctgctgctcc
     4681 agcgcatact tatacagcgc atttttcttc acgccgtgaa tttctgcggc cagcgccgcc
     4741 gcttttttca gcggcagttc tgcctgtagc agcgccagcg tgcgcagggc atcggcgggt
     4801 aagtcttctt cctgtgcttt atgaccttcg acaatcagca ccatttcgcc tttgcgacgg
     4861 ttttcatctt cctttaccca cgccagcagc tcgccaacgg gcgcgccgtg aatggtttcc
     4921 caggttttgg tcagctcacg cgccagaacc acgtagcggg attcgcctaa taccgcaacg
     4981 atatcttcca ggctatctaa cagacggtgg gtagattcat aaaaaatcag cgtgcgcggc
     5041 tccgcttcaa tggcttttag cgcatcacgg cggccttttg atttggcagg taaaaagcct
     5101 tcgtaacaga aacggtcaga gggtaaaccc gcggcgctta acgcagtgat agcagcacac
     5161 ggtccgggta gcggcaccac acgaatcccc gcttcacggc aggtacgcac cagatggtag
     5221 ccaggatcgt taattagcgg cgttccggca tcggaaacca gcgcaatgtt ttgcccctct
     5281 tgcagcttcg ccagcagcgt ttcggctttt tgttgttcgt tatggtcgtg cagcgcaaac
     5341 aaccgggcat taatcccaaa atgttgcagc aataaaccgg tgtgacgagt atcctcggcg
     5401 gcaatcagat caacggcctg taatacctct aacgcacgct gggtgatatc cgccagattg
     5461 ccgattggcg tcggtacaat gtaaagctgg ccctgagaat tatccgccga ttggtgttgt
     5521 ttcattgtgt cgtccgtatt gccgatttaa tattgagcat tgcgtaaaaa aaatatcact
     5581 ggatacatta tggtaccctc aacattttct cgtttgaaag ccgcgcgttg tctgcctgtt
     5641 gttctggcag ccctgatttt cgtcggttgt ggcacccata ctcccgatca gtccactgct
     5701 tatatgcagg gcacggcgca ggctgattct gccttttatc ttcagcagat gcagcaaagc
     5761 tctgatgata ccaggatcaa ctggcaatta ctcgccattc gtgcactggt gaaagaaggt
     5821 aaaaccgggc aggcggttga gttgtttaac caactaccgc aagaactgaa cgattctcag
     5881 cgtcgcgaga aaacactgct ggcggtagag attaaactgg cgcagaaaga ttttgctggc
     5941 gcgcaaaact tgctggcgaa aattacaccc gccgatttag aacaaaacca gcaagcccgt
     6001 tactggcagg caaaaatcga tgccagccag gggcgtcctt ccattgattt actgcgcgcg
     6061 ttaattgctc aggaaccgct gcttggcgcg aaagaaaaac agcagaatat tgatgccacc
     6121 tggcaggcgc tctcctccat gactcaggaa caggcgaata cgctggtgat caacgccgac
     6181 gaaaatattc tgcaaggctg gctggatctg cagcgcgtct ggtttgataa ccgtaacgat
     6241 cccgacatga tgaaagccgg gatcgccgac tggcagaaac gttatccgaa caatccgggc
     6301 gcgaaaatgc tgccaacgca gttggttaac gtaaaagcgt ttaaaccagc ctcgaccaac
     6361 aaaatcgccc tgctgttgcc actgaatggc caggcagcgg tatttggtcg cactattcag
     6421 caaggctttg aagcggcgaa aaatatcggc actcagccag tggcggctca ggtagctgcc
     6481 gcacctgccg cagacgtagc agaacaacct cagccgcaaa ctgcggatgg cgttgccagc
     6541 ccggcacaag cctcggttag cgatctgacc ggtgatcagc ctgcggccca gccggtgcct
     6601 gtaagcgccc cggcgacaag caccgcagcg gtaagcgcac ccgcaaatcc atccgcagag
     6661 ctgaaaatct acgatacctc atcacaacca cttagccaga tcttaagcca ggttcagcag
     6721 gatggcgcga gtattgtggt tggtccgttg ctgaaaaata acgttgaaga gttgctgaag
     6781 agcaacactc cgctgaacgt actagcactg aaccagccgg agaatatcga aaatcgcgtc
     6841 aatatttgtt acttcgcgct ttcaccggaa gacgaagcgc gcgatgcagc gcgtcatatt
     6901 cgtgaccagg gtaaacaagc gccgctggtg ctgatcccac gcagttcatt gggcgatcgc
     6961 gtagccaatg cgtttgcgca agagtggcag aaattgggcg gcggcaccgt tctgcaacaa
     7021 aaatttggtt ccaccagcga attacgcgcg ggtgttaacg gcggttctgg tattgcttta
     7081 acgggtaccc cgattactcc cagagcgaca accgactccg gcatgacgac caacaatcca
     7141 acgctgcaaa ccacgccaac cgatgaccag ttcaccaata atggcggtcg tgtcgatgcg
     7201 gtgtacattg tggcaacgcc gggtgaaatc gcttttatta aaccgatgat cgccatgcgt
     7261 aacggtagcc agagcggtgc aacgctgtac gccagctccc gcagtgcgca agggaccgct
     7321 ggcccggatt tccgtctgga gatggaaggc ttgcagtaca gcgaaatccc gatgctggcg
     7381 ggcggtaatc tgccgttaat gcagcaggca ctcagcgcgg tgaataacga ttattcactg
     7441 gctcgcatgt atgcgatggg cgtcgatgcc tggtcgctgg caaatcattt ctcacaaatg
     7501 cgccaggttc agggttttga aatcaacggt aataccggaa gcctgacggc taacccggat
     7561 tgcgtgatta acaggaagtt atcatggcta cagtaccaac aaggtcaggt agtccccgcc
     7621 agttaaccac caaacagacc ggcgatgcgt gggaagtaca agcgcgtcgc tggctggaag
     7681 gcaaaggact gcgatttgtc gccgctaacg tgaacgagcg tggcggcgag atcgatctga
     7741 taatgcgtga aggccggacc accgtttttg tcgaggtgcg ttaccgccgt tctgcgcttt
     7801 atggcggcgc ggcagccagt gtgacccgca gcaaacaaca caaattatta cagactgccc
     7861 gcttgtggct cgcgcgtcat aatgggagtt ttgatactgt ggattgccgg ttcgatgtgg
     7921 tagccttcac cgggaatgag gttgagtgga ttaaggatac ctttaatgac cactcataat
     7981 taaggtttaa ggattagcgt gcaagaaaga attaaagctt gcttcactga aagcattcaa
     8041 actcaaattg cggcggcaga ggcgcttccg gatgccatct cccgtgcggc catgacgctg
     8101 gttcagtctc tgctcaatgg caacaaaatc ctctgttgtg gtaatggaac ttccgctgcc
     8161 aatgcacagc attttgctgc cagcatgatc aaccgtttcg aaacggagcg gcccagctta
     8221 cctgccattg cactaaatac cgataatgtt gtcttaacgg cgattgccaa cgatcgctta
     8281 catgatgaag tgtatgcaaa acaggtgcgg gcgctggggc atgcgggaga tgtattgtta
     8341 gccatttcca cccgtggcaa cagccgcgat atcgtaaaag cagtagaagc tgccgttacg
     8401 cgtgatatga ccattgtggc attgaccggt tatgacggcg gtgaacttgc aggtttgtta
     8461 gggccacagg atgtggagat ccgcatccct tcgcatcgca gtgctcgcat tcaggaaatg
     8521 catatgctga cggtaaattg cctgtgcgat ctgatcgata acacgctttt ccctcaccag
     8581 gatgattaag gagaatacat gaaggcatta tcgccaatcg cagtccttat ttccgcgctg
     8641 ctgttgcaag gttgtgttgc cgctgccgta gtgggtactg ctgctgtggg taccaaagcc
     8701 gcaactgacc cacgcagtgt cggcacccag gtggacgatg gtaccctgga agtgcgcgtg
     8761 aacagcgcat tgtcgaaaga cgaacagatt aagaaagaag cgcgcattaa tgtaacggcc
     8821 tatcagggca aagtgctgct ggttgggcag tcaccaaacg ctgaactttc ggctcgcgcc
     8881 aaacagattg ctatgggcgt agacggtgcc aacgaagtgt ataacgagat tcgtcagggc
     8941 cagccgattg gtctgggcga agcgtctaac gatacgtgga tcaccaccaa agtgcgttcg
     9001 cagctcttaa ccagcgacct ggtgaaatcg tccaacgtga aagtgaccac cgaaaacggt
     9061 gaagtgttcc tgatggggct ggtgactgaa cgtgaagcga aagcggcggc agatattgcc
     9121 agccgggtga gcggcgtgaa gcgggtaact acggcgttta cgtttattaa atagcagccc
     9181 cttgtaatgc ctgatgcgac gcttgccgcg tcttatcagg cctacaacga tgcaaaccgt
     9241 aggtcggata aggcgtttac gccgcatccg acatgttttc cctcaaatca gaataacagc
     9301 gccagcccgc cgacaatcac gcctgacact gccaccatcg cccccgtcag ccataaggct
     9361 ttcgccggga acgctttgcg cagcataatc agtgacggca aactcaccgc cgggagcgtc
     9421 atcaacaatg ccagcgccgg agcagttccc atacctgcca gcatcatcgt ttgtacaatc
     9481 ggaatttctg ctgccgtggg aatgacaaac aagcaccctg ctaccgccat cgccaccacc
     9541 cacatcaggc tgttatcgac agcaccatcg gcatggggga ataaccagac gcgagcggca
     9601 cccaacacca gtactgcaag gatgtaaacc gggatcgtac tccagaaaag cgtccatagc
     9661 gccctgcccc agcgactaaa aaatccgccc tgtgcttccg gtatgtcaat ttcgaccggt
     9721 gcttgcgttt gcggtgtttc acgcacccat ttttgcacca gcgtcgcaat cagcaacacc
     9781 atcaccagcc cggccaccag acgaatcgcc gcaaaacccc agccgaggac aaaccccata
     9841 aacaccagcg tcgccgggtt taacaccgga ttgcccatcc agaatgccag cgcaccgccc
     9901 atcgacacct gttggcgacg cattcccgcc gcgaccggag ccgcacagca ggtacacatc
     9961 atgcctggca gcgaaaacag cgttcccagc agcgtgccgc gaaagcgcga ttgcccaagc
    10021 gtacgcaaca accagtcacg cgggatcagc acctgaatca acgaaccgag gatcaccccc
    10081 agcaccgccg ctttccatac cgcgaggaaa tagatcatcg cgtaatccaa cgccgcctgc
    10141 catggattag catccgcctg cgcaaggata gatttaccga tactgtgggt ttcggcagca
    10201 gtaaacgctt tgccgtagta aggttgccat ttcacatacc agagaccggc aatgacaacg
    10261 agaaagaaaa gcgcgggttt ccaccactga atgggcgttg ccgcctgaga tgaagactga
    10321 ccagtcatag cattccccag gaataagtta tgataattga gcgcgtgaat attacgctca
    10381 ctatcaattc ttgggaataa ttattcagct cttttgcgta attctgaaga gcttaaaatc
    10441 gtcacacctt catgctcggg ccgcatcgcc tctgccagca tgacccgcgc cacgtctctc
    10501 gcatcaatgg atttccagtt accgggtaac aaacggaaca gcggcgcaaa aagcgtttcg
    10561 ttcatccgct gtttgctacg atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc
    10621 ggccagttct gggcgattaa tgcttcttcc atctcccctt tgacgcggtt atagaaaaac
    10681 ggcgagtggg cattggcacc catagcactg accaccagca tatgctgcgc gcccagtcgc
    10741 cgcccggtta atgcggtatc cactaccagc gtgtaatcgg catgaataaa cgcctctttg
    10801 ctccccgctt ctcgccgcgt ggtgccgaga caacaaaaca caatgtcgat gggatcggtg
    10861 acctgcgcca gcgcgtcagt cagttgcgga tcatggggat taaatacccc gggcatatcg
    10921 cccaacggac gtcgcgtcgg cgcggctatg gcgttaactt tcggttcgtt aatcaacatc
    10981 cgcagcaggt gaccgcccac cagccccgtt gcgcctgtaa tcagtacctg actcatcttc
    11041 gctcctttac agaattgtct gccttgcgct ccacggctca tgcaccaggc ttaatagacc
    11101 atgaggtaat tatccccgat tgtgggaaat tcgcctcatc caatgcaaca acgcggagga
    11161 agcatgagta agaaaattgc cgttttaatc actgatgaat ttgaggattc agaatttact
    11221 tcacccgcag acgagttccg taaagccgga cacgaagtga ttaccattga aaaacaagcg
    11281 ggtaaaacgg tgaaaggcaa aaaaggagaa gccagcgtga ccatcgataa atccatcgat
    11341 gaagtgacgc cagcggagtt tgatgccctg ttgctaccgg gcggtcattc accggattat
    11401 ctgcgtggcg acaaccgttt tgtcaccttt acccgtgatt ttgtgaatag cggcaaacct
    11461 gttttcgcca tctgtcacgg tccgcagctg ctgatcagcg ccgatgtgat tcgggggcgc
    11521 aaactgaccg cggttaaacc gatcattatt gatgttaaaa acgcgggcgc ggaattttac
    11581 gatcaggaag tcgtggtcga taaagatcag ctggttacca gccggacacc ggacgatctg
    11641 ccagcgttta accgcgaggc gttacgcctg ctcggtgcct gagccgcgta gccaaatcat
    11701 ttttttacca aagcccagcg tgttgtcggt gaatttgatt tcatcgggcc ggatttccca
    11761 caccggggcc gacagcattc tggcaaccgg aaagcgacga ttgtacgcct tgcgcgcgag
    11821 gtcgctttcc tcaccttcca gcctgcggat ctcaccttta aactgcacgc cgcgaattaa
    11881 cgctaccgtt ttcggctgac cgtttaccgt tccggcaacc gcagcctgcg gcccacgcat
    11941 ctgcgcgtgg cgcgtttttt cttccgtcaa aatgtagaag gcgactttct gcacatcaaa
    12001 cagataaaag gcattggcgc accagagttc cccttcctgc tgcacacacc aggtgacaac
    12061 atgttgtttt gccagccaac ggctgatggc ggtgagtgtt tccattgctg ttctctttta
    12121 tactgtgggc ctgaccttaa catgcgacgc agacgatgac accctggttt ctttacttga
    12181 tccgtaccgc cgacaataag ctttataccg ggatcaccac ggatgtcgaa cgccgctatc
    12241 agcagcacca aagcggcaaa ggggcgaaag cactgcgcgg gaaaggagaa ctgacgctgg
    12301 cgttttctgc gccagtgggc gatcgttcgc tggcgttacg ggcggaatat cgcgttaagc
    12361 aactgaccaa acggcaaaaa gagcgtctgg tagcggaagg cgcagggttt gcaaagctgt
    12421 taagcagtct gcaaaccccg gagattaaaa gcgattgaaa tgctcgtgat actcaaccag
    12481 accagttacg ccattcaacg cgtcatccgc taaacgatgt acctggaagg cgctttcagt
    12541 gcccggccag cggcaacgca gatcgtgatg cgccgccagt tcaaagccga aacggctgta
    12601 tagcgccgga tcgcccagcg tcaccactgc ggcatagccg aactcattaa gcgaatcgag
    12661 tccttcatag accagttggc gtgccagccc ctgtccacgg tatttttcat ctaccgccag
    12721 cggtgccatg ccgacccatt gcaggtcttc gccctgcaca tcaaccggac taaatgccac
    12781 atagccaatg acctgacctt cgtcatctgt cgccaccagc cccagcgtca gaaagccatc
    12841 ttcacgcaga tcgtgaacca gcttcgcttc cgcatcactt tcgaatgaac gacgcagcag
    12901 ggcatcaata cccggcgcat caatgggaat ttctactcga attagcatgg ttcacctacc
    12961 gatgtctgtt tggtttcagg cgcggttttc attcccgcct caacaaaatc cgccagttgc
    13021 agcagcatca tgcgtaaggc tttcggcatt tgctccagtt caatggcgtc catcaggttt
    13081 ttcacataca gccccagctc cgtatcgcct tcaatcacca gccgacgctg gaagaagagc
    13141 gtatccggat cttgtttacg cgccgcaatc atcagcagat cgctggcgtc ggcactaaaa
    13201 ctcacatcag cttgcgcgtt ctggctgacc accagtttgc cattcaccac cgaggtaaac
    13261 cattgcaggt caatatcacg cacatgaata cttaaccagc ggccttcaag aaactccagc
    13321 tcaccatcat ccagcgcctg gcggaattgc cagcttaaga cctgctcaag aacctggcgt
    13381 tttagcgcaa atggcgtcag ttttaccggt acactcaaca gagatggccc caaatgcaca
    13441 atacgggaac gcagtttatc caacacgagt tttactccct gtttcaacaa tcatcctatt
    13501 ttgccatatc agaaaaataa catagcggta taaatcaaca attccatatg aaattgctgc
    13561 taccaccaat acaactttaa ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa
    13621 atcccagttc gttaacattt ttgcgttttg atagcgcaac cttcaggaaa aattatggag
    13681 ctgctctgcc ctgccggaaa tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat
    13741 gctgtttata tcgggctaaa agatgatacc aatgcccgtc acttcgccgg ccttaacttt
    13801 accgagaaaa aattgcagga agcggtgagt tttgtccatc aacatcgccg caaacttcac
    13861 atcgcgatta acacttttgc gcatccggac ggttacgccc gttggcagcg tgccgtggat
    13921 atggcggcgc agctgggtgc cgacgcgctg atcctcgccg acctcgccat gctagagtac
    13981 gccgccgagc gttacccgca tattgagcgt catgtgtcag tgcaggcttc ggcgaccaat
    14041 gaagaggcga ttaactttta tcatcgccat tttgacgttg ctcgcgtggt gctgccgcgc
    14101 gtgttgtcga ttcatcaggt gaaacaactg gcacgggtca cacctgtccc gctggaagtc
    14161 tttgctttcg gcagcctgtg cattatgtcg gaaggtcgtt gctatctgtc gtcgtatctg
    14221 acgggtgagt cgcccaacac cgtgggcgcg tgttctccgg cccgtttcgt gcgctggcag
    14281 caaacgccgc aggggctgga atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac
    14341 ggcgaaaacg caggttatcc gacgctatgt aaagggcgtt atctggtgga cggcgagcgc
    14401 tatcacgcgc tggaagaacc aaccagtctc aataccctgg aactgctgcc ggagttaatg
    14461 gcggcgaata ttgcttcggt gaaaattgaa ggccgccaac gtagcccggc gtatgtcagc
    14521 caggtggcga aagtctggcg tcaggctatc gaccgttgta aggccgatcc gcaaaacttc
    14581 gtaccgcaaa gcgcgtggat ggagacgctc gggtcgatgt ccgaaggcac gcagaccacc
    14641 cttggcgcgt atcaccgtaa atggcagtga gaagagcaat gaaatattcc ttagggccag
    14701 tgctgtggta ctggccaaaa gagacgctgg aagattttta tcagcaggcc gccaccagca
    14761 gcgccgacgt gatttatctt ggcgaagcgg tttgcagcaa gcgccgggca accaaagttg
    14821 gcgactggct ggagatggca aaatcgctcg ccggaagtgg taagcagatt gtgctctcca
    14881 cgctggcgct ggtgcaggca tcatctgaac tgggcgaact gaaacgctat gttgagaacg
    14941 gtgagtttct gattgaagcc agcgatctcg gcgtggtgaa tatgtgcgct gaacgcaaac
    15001 tgccgttcgt cgccggacac gcgctgaact gctataacgc ggtgacgctg aaaatattgc
    15061 tcaaacaggg catgatgcgc tggtgtatgc cggtggagct ttcccgcgac tggctggtga
    15121 atctgcttaa tcagtgcgat gagctgggca ttcgcaacca gtttgaagtt gaagtcctga
    15181 gctacggtca tctaccgctg gcctactccg ctcgctgctt taccgcgcgt tcggaagacc
    15241 gcccgaaaga tgagtgtgaa acctgctgca ttaagtatcc gaacgggcgc aacgtgctgt
    15301 cgcaggaaaa ccaacaagtg tttgtactca atggcattca gaccatgagc ggctacgttt
    15361 acaacctcgg taacgagctg gcatccatgc agggcctggt tgatgtggtc cgcctgtcac
    15421 cgcagggtac tgacactttc gcgatgctcg acgccttccg cgctaatgaa aatggcgctg
    15481 caccactgcc gttgacggcg aacagcgact gtaacggcta ctggcggcgg ctggcaggac
    15541 tggagctgca agcctaagta aatagctcac tttgttaaca actttaacta ctctttaatg
    15601 cagtattaaa gattaatcgg taacaaagtg agctgttatg actgataaaa ccattgcgtt
    15661 ttcgctactc gatctggccc ccattcccga aggttcttca gcgcgagatg cattctccca
    15721 ctctctcgat ctcgcccgtc tggctgaaaa gcgcggctat catcgctact ggctggcaga
    15781 acaccacaat atgactggca ttgccagtgc tgccacgtcg gtattgattg gctatctggc
    15841 ggcgaatacc accacgctgc atctggggtc tggcggcgtg atgttgccta accactcacc
    15901 gttggtcatt gcagaacagt tcggcacgct caatacactc tatccggggc gaatcgattt
    15961 ggggctgggt cgtgctccgg gtagtgacca gcgaaccatg atggcgctac gtcgtcatat
    16021 gagcggggat attgataatt tcccccgcga tgtcgcggag ctggtggcct ggtttgacgc
    16081 ccgcgatccc aatccgcatg tgcgcccggt accaggctac ggcgagcaaa tccccgtgtg
    16141 gttgttaggc tccagccttt acagcgcgca actggcggcg cagcttggtc tgccgtttgc
    16201 gtttgcctca cacttcgcgc cggatatgtt gttccaggcg ctgcatcttt atcgcagcaa
    16261 cttcaaaccg tcggcacgac tggaaaaacc atacgcgatg gtgtgcatca atattatcgc
    16321 tgccgacagc aaccgcgacg ctgaatttct gtttacctca atgcagcaag cctttgtgaa
    16381 gctgcgccgt ggcgaaaccg ggcaactgcc gccgccgatt caaaatatgg atcagttctg
    16441 gtcaccgtct gagcagtatg gcgtgcaaca ggcgctgagt atgtcgctgg tgggcgataa
    16501 agcgaaagtg cgtcatggtt tgcagtcgat cctgcgcgaa accgacgccg atgagattat
    16561 ggttaacggg cagattttcg accaccaggc gcgcctgcat tcgtttgagc tggcgatgga
    16621 tgttaaggaa gagttgttgg gatagtgtgt cttaacgcgg gaagccttat ccgagctggc
    16681 aacgctgtcc cacgtaggcc tgataagcga agcgcatcag gcattgtgta ggcagcagaa
    16741 atgtcggata aggcccgctg attactgata caccggcagc aaattaaagc tcgataagat
    16801 atggaccagt gcgttgccaa cgccaaatac cagaatcagc gtaatcatcg gcttgccgcc
    16861 ccagacgcgg aatttcgggc tgccaaagcg tttacgcgat gcgcgggcca gcagtgccgg
    16921 aacaattgcc gcccagatgg tagctgctaa accagcataa ccaatggcgt acaggaatcc
    16981 gttcgggaac agcagaccac ccacaacagg aggggcaaag gtcagcaatg ccgttttcaa
    17041 gcggcccaca gccgagtcgt cgaaaccaaa cagatctgcc agatagtcaa acaaacccag
    17101 cgttacgccg aggaacgaac tcgctaccgc aaagtttgag aacacgacca gcagcagatc
    17161 cagactacgg ctgttcagta cgccgcttaa cgcctgtacc agcacatcaa tattaccgcc
    17221 cttctctgca atatcgataa actccggacg cgggatgtta cccatcgtcg ccagcaacca
    17281 gatggtatac agcgccagcg ccatcagcgt accgtacacc agacatttca cgatggtttt
    17341 cggatctttg ccgtaatact tcatcaggct tggcacgtta ccgtgataac caaacgatgc
    17401 cagacagaac ggcaaggtca tcaacagata cggtgcataa gacgcattgc tttcagcgac
    17461 gttgaacaat gtcgcaggct gcacatgccc cagcaggcta ccgaaggtga ggaagaaggt
    17521 aatgactttc gcccccaata caatcgctgt catgcgactg acggctttag tgctcaacca
    17581 caccacaaac gctaccagca atgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag
    17641 tgacatctct gcgaaggtgt gatgcagaat cgaaccactg gcagaaatat aggcataggt
    17701 caggatatag agcacaaagg caatggaaat gccgttgacc acgttccagc ctttgcccag
    17761 caaatctttg gtgatggtgt caaaactcga accgattctg taattcaggt tagcttccag
    17821 aatcatcaag ccggaatgca gcatacagaa ccaggtaaag atcagcgccg ccattgacca
    17881 gaaaaaccac gccccggaca tgaccactgg cagagaaaac atccctgcgc caataatggt
    17941 gccgccgata atcaccacgc cgccaagcag cgacggtgac gtttgggtgg tggttagtgt
    18001 tgccatgagg gcttctctcc agtgaaaaat agtgcgactg cgttgttatg tattgcactg
    18061 taccagtacg cgagtacaaa agacagaaaa aaagccccga tggttcaaat cggggctgta
    18121 tatattattt tacagactgt gttcgctgtt cagcgatgat tacgcatcac caccgaaacg
    18181 acgacgaccg gtagagtcat cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa
    18241 acgacgacca tcaccacgac caccttcacg gcgttcaccg ctgaagttac gaccaccttc
    18301 acgacgctcg ccactgaaac cacgaccacc gccacgacgc tctccgccag tatgcggctg
    18361 tgcatcgccc agtaattgca tattcatcgg cttgttcaga atgcgagtac gtgtaaagtg
    18421 ttgcagcact tcacccggca tacctttcgg cagttcgatg gtggagtgag aagcaaacag
    18481 cttaatgtta ccgatgtaac ggctgctgat gtcgccttcg ttagcaatcg caccaacgat
    18541 atgacgaact tcaacaccat catcgcggcc cacttcaata cggtacaact gcatgtcacc
    18601 aacatcacga cgttcacgac gcggacgatc ttcacggtcg ccacgcggac cacggtcgtt
    18661 acgatcgcgc ggaccacggt catcacggtc acggaattca cgtttcggac gcatcggcgc
    18721 atctggcgga acaatcagag tacgttcacc ctgtgccatt ttcagcagtg cggcggccag
    18781 agtttcgaga tccagctctt caccttctgc agtcggctga attttgctca gcagtgcgcg
    18841 gtattgatcc agatcgctgc tttccagctg ctgctgtact ttagcggcga atttttccag
    18901 acggcgtttg cccagcagtt ctgcgttcgg cagttctact tccggaatag tcagcttcat
    18961 agtacgttca atgttgcgca gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg
    19021 gccagcacga cccgcacgac cggtacgacc gatacggtga acgtaagact cagaatccat
    19081 cgggatatcg tagttaacca ccaggctgat acgctcaacg tccaggccac gggctgcaac
    19141 gtcggtcgca atcaggatgt ccagacgacc atctttcagg cgttccagtg tctgctcacg
    19201 cagcgcctgg ttcatgtcac cgttcagcgc ggcgctgttg tagccgttac gctcaagagc
    19261 ttcagccact tccagagtcg catttttggt acgaacgaag ataatcgccg catcaaaatc
    19321 ttccgcttcc aggaaacgta ccagtgcttc gtttttacgc ataccccaga cagtccaata
    19381 gctctggctg atgtcaggac gggtagtcac gctggactga atgcgcactt cctgcggctc
    19441 tttcataaag cggcgggtaa tgcgacgaat cgcttccggc atggttgcag agaacagagc
    19501 ggtctgatga ccttccggga tctgcgccat aatagtttca acgtcttcga tgaagcccat
    19561 gcgcagcatt tcgtcagctt catccagaac cagaccgctc agtttagaga ggtccagagt
    19621 gccacgtttc aggtggtcca gcagacggcc cggagtaccg acaacgatct gcggcccctg
    19681 acgcagggcg cgtaattgca cgtcataacg ctggccgccg tacagggcaa ccacatttac
    19741 gccgcgcatg tgtttagaga aatccgtcat tgcttcagca acctgtaccg ccagttcgcg
    19801 ggtcggtgct agcaccagaa tctgtggtgc tttcagctca ggatcaagat tttgcagcag
    19861 aggtaaagag aatgctgcag tttttccgct ccccgtctgg gccataccca gtacgtcgcg
    19921 gccattcagc agatgtggaa tacactccgc ctgaattgga gatggttttt cgtaacccag
    19981 atcgttaagg gcttcaagga taggagcctt caggcccaga tctgcaaaag tggtttcgaa
    20041 ttcagccatg tagtacgtgt gcctcaaaat taatggcggc cagtctacat aactcatcat
    20101 gaaattgatc agcaattttc attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg
    20161 aacaacaacg ccctcacccg ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg
    20221 tacgtcagct attgctggtc cgattctgcc aggtcatctt ggtcctggcc caggagcgat
    20281 aattccaaca atgcgtatcg gtgctcaaca aagttatgaa cgttgttggc aaccgccagt
    20341 ttgaacagtg ccgtggcgct gtccaaatcc cccagactta ggtagtactt acctaaatag
    20401 aagttggttt cactgagatg ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc
    20461 ctttccatta acgtttgttc gctaatgttg cccaggtaga actcgacaat gttccatccc
    20521 cactgttcct tatccgattt ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca
    20581 tcgagcttct gctcggcgag ataaagccac agactacgga aaggatcatt gggatcgtct
    20641 tgataaaacg ccagcagatc atcttgcgct aacttgtcac gaccgccgta atataatgcg
    20701 atcccgcgat tcaagtgcgc gtagttgtaa gttggatcaa gctcaagtac agaatcaaac
    20761 gcttcatagg cagcatcaaa attgcctgcc tgcgttaaat atatgcctaa gtaattgaat
    20821 acttcaggca tatccggtcg gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc
    20881 ctcagaccga gactatcata caacactccg cgctcatata aaagctgtgc gcgttcgtca
    20941 tcggttaaag cccgactggc aaggatttgt tccatacgtg ccagaatcac ttcctgctgt
    21001 aaagtcggtt gcaatggtac cgcgaggact tcacttttac gccaggaagt attactgcat
    21061 cctgcaagcg taagtgctgt cgcaacgaaa caccagcgca aaaaaggctt catttcccac
    21121 tcccgaagac cacggttgaa tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc
    21181 ggttaaaagc cccccgccgc agcggagggc aaatggcaac cttactcgcc ctgttcagca
    21241 gccggagctt ccggtgctgc agcaggttga gactgctcag tcgcttcttt aatgctcaga
    21301 cggatacggc cctggcgatc aacttccaga actttcaccg gtacttcctg acccatctgc
    21361 aggtaatcgg tcactttctc aacgcgtttg tcagcgattt gagagatgtg gaccagacct
    21421 tctttaccgc cgccgatggc aacaaatgcg ccgaagtcaa cgatacgggt cactttacca
    21481 gtgtagacgc ggcccacttc aatttctgca gtgatctctt cgatacgacg aatagcgtgt
    21541 ttcgctttct cgccatcggt cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg
    21601 gtggtgccag tttcttcggt cagggcacgg atcacagagc cgcctttacc gataacgtct
    21661 ttgatcttgt ccgggttgat cttgatggta tggatacgcg gagcgaactc agagatatcg
    21721 ccacgtggcg cgttgatcgc ctgttccatt acacccagga tgtgcagacg cgcaccttta
    21781 gcctggttca gtgcaacctg catgatctct ttggtgatac cttcaatttt gatatccatc
    21841 tgcagtgcag agataccgtc gcgggaaccc gcaactttga agtccatatc gcccaggtga
    21901 tcttcgtcgc ccaaaatgtc agacagtaca acgtagttgt cgccttcttt caccagaccc
    21961 attgcgatac ccgcaacggc agctttgatt ggcacacctg cgtccatcag cgccagagac
    22021 gcaccgcaca cggaagccat agaagaagaa ccgttggatt cagtgatttc agacacaaca
    22081 cgtacggtgt acgggaattt gtccatatcc ggcattactg ccagcacgcc gcgcttcgcc
    22141 agacgaccgt gaccaatttc acgacgcttc ggagaaccga ccatgccggt ttcgcctacg
    22201 gagtacggag ggaagttgta gtggaacagg aaggtatcgg tacgttcgcc catcagttca
    22261 tcaagaacct gcgcgtcacg agcagtaccc agcgttgcgg taaccagcgc ctgcgtttca
    22321 ccacgggtga acagcgcaga accgtgagta cgcggcagca cgccagtacg cacatccaga
    22381 ccacggatca tatctttttc acgaccatcg atacgcggtt cgcctgccag tacgcggcta
    22441 cgaacaacgt ttttctcgat cgcgtgcaga atttcaccca gttcgttttc gtccagggtt
    22501 tcgtcttcag caagcagcgt cgcgatggtt tcagatttga tgacatcaac ctgcgcataa
    22561 cgctcttgtt tgtcggtgat gcggtaagca tcgctcagac gagcttcagc cagtgcagca
    22621 acgcgcgcgt tcagcgcttc gtttaccggc tccggctgcc agtcccaacg cggtttaccg
    22681 gcttctttca ccagttcatt gatgttctga ataacaacct gctgttgttc atgaccgaac
    22741 actactgcgc ccagcatctg gtcttcgctc agcagttcag cttcagattc aaccatcagt
    22801 acagcggctt cagtacccgc aacaaccaga tccagtttgc tctctttcag ctcgtcctga
    22861 gtcgggttca gtacgtactg gtcattgatg taacctacgc gggcagcacc aatcgggcca
    22921 ttgaacggaa taccagacag agacagtgct gcggaagcac caatcatcgc gacgatatcc
    22981 gggttaacct gcgggttaac agaaaccacg gtggcgataa cctgaacttc gttgacgaag
    23041 ccttccggga acagcgggcg aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct
    23101 tcgcttggac gaccttcacg acggaagaag ctacccggga tacgaccagc agcgtaggta
    23161 cgctcctgat agttaacggt cagtgggaag aagtcctgac ctggtttggc ttttttctgg
    23221 ccaacaacgg taacaaatac cgcggtgtca tccatgctaa ccataacagc ggcagtagcc
    23281 tgacgagcca tcatgccggt ttccagagtc acggtgtgtt ggccgtactg gaatttacga
    23341 acgatcggat taagcaatgt aatatccttt ctctttctta gacagtacct tacggcactg
    23401 gtgttaatac ccgatcttct gcgcatcctc gcgactaatg acaaccctaa cccagctcta
    23461 tgtgggtaaa gcctctcatt agccgcgcga acctctgcaa cggaagatca ttcatagcaa
    23521 caatacatta gtttccagtg aattgctgcc gtcagcttga aaaaaggggc cactcaggcc
    23581 cccttttctg aaactcgcaa gaattagcga cgcagaccca ggcgctcgat gagccgggtg
    23641 taacgtgcta cgtctttacg tttcaggtag tcgagcagtt tacgacgctg agaaaccatg
    23701 cgcagcagac cacgacggct gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg
    23761 tggttgatct gtgcagtcag cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg
    23821 tcacgaccaa actcagaaac gattttagct gttgcttcag tacttagaga cattttaaaa
    23881 ctccaaagta tatagaatga aaagacgccg atctctaatt cagcgatccc agtgtacgtt
    23941 acgcaaagtg ttaaacaatt tacgcgacgt taagcggcag tattctactc gtagcgacct
    24001 gttatcgcaa gacggttaac attacgccgg gtattcaacc accagacgac gaggcgcaac
    24061 gcggccttca tcgtcaattt cgcccatacc gataaacttg ccgttctcac cttccgtaac
    24121 gcgaaccagt ccttccagcg gcgcgccaga tgtacgaacc gggttaccat ttttgaagta
    24181 aacagaagac gttaacggaa gattcaccac cgggtagtcc gaagctggac tgtccattgg
    24241 catcagtaat ggatcaagca actccgcagc cggaatatcc tgctgttcgg cttgttcgac
    24301 cagttcacgc aggtgctcca gggtcaccat ccgttcaacc ggatatttac ttaccgccag
    24361 acgacgcagg taaatcacat gcgcgccaca gccgagtttt tcacccaggt catcaatgat
    24421 ggtgcggata taagtgcctt ttgagcagtg aatttccagc tccagctcat tgccttcatg
    24481 gcgaataaac agcaattcat aaacggtaat tggacgcgct tcacgcggaa cttcaatgcc
    24541 ctgacgcgca tattcgtaca gttttttgcc ctggtatttc agtgctgaat acatcgatgg
    24601 gatctgttcg atatcgccac ggaaagtatc cagtgccgcc gccagttgtt ctgcactaaa
    24661 ggttaccgga cgctcttcaa cgatttgtcc gtcgacgtca gaagtatcgg tacgctgtcc
    24721 aagacgcgca atgacccgat agcgtttgtc ggagtccagc agatactggg aaaacttcgt
    24781 cgcttccccg aggcaaatcg gcaacatgcc ggtcgccagc gggtccagcg caccggtatg
    24841 cccggcacga ttggcgttat atatacgttt cactttttgc agcgcatcgt tgctggacat
    24901 accctgcggt ttatccagca acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg
    24961 actcattagt cctccttgct gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc
    25021 acgctggtca ccaggtttga catacgcatc ccttcaacca gagagttgtc gtagaagaag
    25081 gtcagttccg gcacgatacg caggcgcatc gctttaccca gcaggctgcg gatgaaacca
    25141 gaagcttcct gcaacgcttt gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg
    25201 aaagtcacat acactttggc atacgccagg tcgcgagaca tttcgacacc ggaaacggtg
    25261 gtcatcatgc ccagacgagg atctttaatt tcacgctgca ggatgagagc gatctctttt
    25321 tgcatttcct gcgctacgcg ctgcgggcga ccaaattctt tcgccataat aaattctcct
    25381 gacaaaaaag gggctgttag ccccttttta aaaattaatt tcaggtggaa gggctgttca
    25441 cgttggcctg ataagacgcg ccagcgtcac atcaggcaat ccatgccgga tgcggcgtaa
    25501 acgccttatc ccgcatggaa ccctaaaaac cttaagcgat ggtacgttgg atctcgatga
    25561 tttcgaatac ttcgatcaca tcgccagtgc ggacgtcgtt gtagttctta acgccgatac
    25621 cacattccat accgttacgg acttcgttaa cgtcatcttt gaagcggcgc agggactcca
    25681 gctcgccttc atagataacc acgttgtcac gcagaacgcg gatcgggttg tgacgtttaa
    25741 ccacaccttc ggtaaccata cagcctgcga tggcaccaaa tttcggcgat ttgaacacgt
    25801 cacgaacttc cgccagaccg ataatctgct gtttcagttc cggagacagc ataccgctca
    25861 tcgccgcttt cacttcgtcg atcaggttat agatgacgga gtagtaacgc agatccaggc
    25921 tttccgcttc aatcacttta cgtgcagagg catcagcacg tacgttgaag ccaaccagga
    25981 tggcgttgga cgccgcagcc aaggtggcgt cggtttcggt gataccacct acgccagaac
    26041 cgatgatctt cactttaact tcgtcagtag acagtttcag caaggagtcg gagatcgctt
    26101 cgacagaacc ctgtacgtct gccttcagga cgatattcac ttcgtgaact tcgccttcgg
    26161 tcatgttggc gaacatgttc tcgagtttag atttctgctg acgcgccagt ttaacttcgc
    26221 ggaatttacc ctgacgatag agtgcaactt cacgcgcttt cttctcgtca cgtacaacgg
    26281 taacttcatc acccgcagcc ggaacaccgg acaagccgag gatttccacc ggaatggacg
    26341 gacccgcttc cagcacttcc tgacccagtt cgttacgcat cgcacgaaca cgaccgtatt
    26401 cgaagccaca cagaacgata tcgcccttgt gcagagtacc ttcacgtacc agaacggtag
    26461 caaccggacc acgaccttta tcgaggaagg attcgataac cgcaccgctc gccatacctt
    26521 tacgtaccgc tttcagctcc agaacttccg cctgcagcag gatagcgtcc agcagttcat
    26581 cgataccggt acccgctttc gcagatacgt gtacgaactg gctttcaccg ccccactctt
    26641 ccggcaggat gccgtactgg gagagttcgt tcttaacgcg atccggatcg gcttctggtt
    26701 tatcgatctt gttcactgca accaccaccg gtacctgcgc cgctttcgcg tgctggattg
    26761 cttcgatggt ctgcggcatt acgccgtcgt cggcagcaac aaccaggact acgatgtcag
    26821 ttgcctgcgc accacgtgca cgcattgaag taaacgcggc gtgccccggg gtgtccagga
    26881 aggtgatcat gccgttttca gtttcaacgt ggtatgcacc aatgtgctgg gtaatgccgc
    26941 ccgcttcgcc agaggccact ttcgttgaac gaatgtagtc cagcagagag gttttaccgt
    27001 ggtcaacgtg acccatgatg gtcacaaccg gagcgcgcgg ttcagccgca gctcccgtgt
    27061 cacggtcgct cattaccgcc tcttccagct cgttttcacg acgcaggata actttgtggc
    27121 ccatctcttc agcaaccagc tgtgcggttt cctgatcgat aacctggttg atggttgcca
    27181 ttgcgcccag tttcatcatc gctttgatga cctgagagcc tttaaccgcc atcttgttcg
    27241 ccagttcgcc aacggtgata gtttcgccga tcacaacgtc acggttaacg gcctgagcag
    27301 gcttctggaa gccttgctgc agcgaagaac ctttacgttt tccgccctta ccgccacgta
    27361 ctgctgcgcg tgcttcttca cgatcagctt ttgattcagc gtgtttgttg cctttcttcg
    27421 gacgcgctgc tttcgcgtta cgaccacggc cacggccgcc ttcgacttca cgatcgcttt
    27481 cgtcttctgc ctggcgagca tgttgagaag tagtgacgtg ataatcgctg gaatcctcag
    27541 tcggttccgc gttatcagtc catttgtttt cttccgccat acgacgtgct tcttcagcaa
    27601 cgcgacgtgc ttcttcttcg agtttacgac gcgcttcttc ttcggcttta cgcttgagct
    27661 ctgcagcttc ctgctcacgg cgggcttttt cagcctgggc gtttttagtc atatcgtctt
    27721 gttgattgct cactttgtct ttttccgcag cttcacgttt cgcttgttca gcagcttcac
    27781 gcttagcttg ttctgcggcc tcacgttcag ctttttgttg cgcctcgcgt ttagccgatt
    27841 cttctgcctc acgacgggct tgctcttccg cttcacgctg cgcttgctct tccgctgcaa
    27901 ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg acttcgattt
    27961 gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt gttttacgtt
    28021 gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc aaagtctgtt
    28081 tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca gcaaattgct
    28141 gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc gttacatctg
    28201 tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag caaatattac
    28261 gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata tcagccagat
    28321 catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg gccagtttga
    28381 atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt ttgttatcac
    28441 cgaggctttc ttcctggacc tgtgcaatgg tggccagtgc atttttagca cgctcgcgca
    28501 gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc atcggcacat
    28561 aggccagttc ttccagcgtc gagaagcctt cctctaccag aacagtcgcg aagtcttcgt
    28621 cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc tgatgcttag
    28681 cctgcaggtc gtcaacggtc atcacgttga gttcccaacc gctcagctgc gaagccagac
    28741 gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accggcttca acggcgatat
    28801 ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt gccattgcgt
    28861 taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc tcgccgccca
    28921 gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca cctaccggat
    28981 cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg tcgcgagccg
    29041 ctgctttaat ttcaatcact tcttcgccga tttctggcac ttcaatacgg aacagttcga
    29101 tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct tccgggcgaa
    29161 cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc ggcagcatat
    29221 cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg ttgtcgcggt
    29281 ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac tgatcaacca
    29341 ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc gtctgggtgg
    29401 tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc aggttcaggc
    29461 tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg gtgacttcat
    29521 caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg atctgtacgc
    29581 ggacgtcgat ctcttgttca tattttttct ttgttgctgt cgccagcgcg ctttccaatg
    29641 cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca actacagcca
    29701 aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag tggggaacca
    29761 agttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg acggtaactg
    29821 tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg ttttgtaccg
    29881 ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt tcagccgtga
    29941 acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg gtgatgggat
    30001 cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca tcaacattga
    30061 tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga ataaattcga
    30121 tgccaaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct gttaattttt
    30181 gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag cccagttatt
    30241 ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg aaccctataa
    30301 ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac tacgaaggcc
    30361 gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg ggggccggat
    30421 ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgc
    30481 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
    30541 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccacgcca cattttgtca
    30601 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
    30661 gccttctcct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
    30721 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
    30781 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
    30841 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
    30901 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
    30961 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
    31021 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
    31081 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
    31141 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
    31201 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
    31261 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
    31321 actgggcggt cgtcatgaaa tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
    31381 gtctgtcgaa aaagcttact ccacggactc caacatgctt ggtgcaacgc atgaagcgaa
    31441 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
    31501 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
    31561 tccggtggcg ttgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
    31621 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgaaaacc gtatcatcga
    31681 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
    31741 acgcctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggccg
    31801 tcagttgggc cgtctgctgt accaggggcg ttggtttgat tcccaggcgc tgatgctgcg
    31861 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
    31921 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
    31981 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta tcggtcaact
    32041 gaccatgcgc aacctggata tcaccgatac ccgcgagaaa cttttcggtt atgccaaaac
    32101 tggcctgctt tcctcctctg ccgcttcagg cgtgccgcag atggagaatc tggaaaacaa
    32161 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
    32221 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaaccagt
    32281 gagtctcctt tatagtgtac aatcacttcc tcaagcgttt gcgactgttc cggctttgtt
    32341 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc
    32401 gttgtgcgat ccacaccatc cccaagaacg gggctatacc agataaacat cggaacatga
    32461 tatgcctgct ggctggcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
    32521 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
    32581 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
    32641 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
    32701 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
    32761 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcacgc
    32821 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
    32881 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
    32941 tcatggctca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
    33001 gcaccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc gacttgcggt
    33061 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
    33121 atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata cggcactgtg
    33181 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata attgagattg
    33241 aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa cgcattttta
    33301 tttttggcat ctttataagc aaattgacaa gcggaaaata aacttcccga aatgacaatc
    33361 agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattaca
    33421 gccaaaaaaa gaagggataa aaaggcaaaa gcacatagat aagggctata cattcccagc
    33481 attttggcaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa
    33541 gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc agtcagtaac
    33601 acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt aacaaccagg
    33661 atggtcagaa cgaagaacag caatcgctga accatatgcg ctccggaaga ggcg
//
LOCUS       DBFIFS010000029        33662 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.029, whole genome shotgun sequence.
ACCESSION   DBFIFS010000029 DBFIFS010000000
VERSION     DBFIFS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.029"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(62..1243)
                     /locus_tag="N7A07_001284"
     CDS             complement(62..1243)
                     /locus_tag="N7A07_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2475978.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(1439..1533)
                     /locus_tag="N7A07_001285"
     tRNA            complement(1439..1533)
                     /locus_tag="N7A07_001285"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Sec,seq:tca)
     gene            1826..3208
                     /locus_tag="N7A07_001286"
     CDS             1826..3208
                     /locus_tag="N7A07_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU2475979.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3218..5536
                     /gene="yicI"
                     /locus_tag="N7A07_001287"
     CDS             3218..5536
                     /gene="yicI"
                     /locus_tag="N7A07_001287"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU2475980.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5589..7298)
                     /locus_tag="N7A07_001288"
     CDS             complement(5589..7298)
                     /locus_tag="N7A07_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU2475981.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="N7A07_001289"
     CDS             complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="N7A07_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU2475982.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9090..10295
                     /gene="gltS"
                     /locus_tag="N7A07_001290"
     CDS             9090..10295
                     /gene="gltS"
                     /locus_tag="N7A07_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU2475983.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10298..11164
                     /locus_tag="N7A07_001291"
     CDS             10298..11164
                     /locus_tag="N7A07_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2475984.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11149..13230)
                     /gene="recG"
                     /locus_tag="N7A07_001292"
     CDS             complement(11149..13230)
                     /gene="recG"
                     /locus_tag="N7A07_001292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU2475985.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="N7A07_001293"
     CDS             complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="N7A07_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU2475986.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="N7A07_001294"
     CDS             complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="N7A07_001294"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU2475987.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="N7A07_001295"
     CDS             complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="N7A07_001295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU2475988.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="N7A07_001296"
     CDS             complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="N7A07_001296"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU2475989.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17270..18952
                     /gene="ligB"
                     /locus_tag="N7A07_001297"
     CDS             17270..18952
                     /gene="ligB"
                     /locus_tag="N7A07_001297"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU2475990.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18949..19566)
                     /locus_tag="N7A07_001298"
     CDS             complement(18949..19566)
                     /locus_tag="N7A07_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU2475991.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="N7A07_001299"
     CDS             complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="N7A07_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU2475992.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20903..21766)
                     /locus_tag="N7A07_001300"
     CDS             complement(20903..21766)
                     /locus_tag="N7A07_001300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU2475993.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21893..22609
                     /gene="rph"
                     /locus_tag="N7A07_001301"
     CDS             21893..22609
                     /gene="rph"
                     /locus_tag="N7A07_001301"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU2475994.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22675..23316
                     /gene="pyrE"
                     /locus_tag="N7A07_001302"
     CDS             22675..23316
                     /gene="pyrE"
                     /locus_tag="N7A07_001302"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU2475995.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23353..23949)
                     /gene="slmA"
                     /locus_tag="N7A07_001303"
     CDS             complement(23353..23949)
                     /gene="slmA"
                     /locus_tag="N7A07_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU2475996.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24056..24514)
                     /gene="dut"
                     /locus_tag="N7A07_001304"
     CDS             complement(24056..24514)
                     /gene="dut"
                     /locus_tag="N7A07_001304"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU2475997.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24492..25712)
                     /gene="coaBC"
                     /locus_tag="N7A07_001305"
     CDS             complement(24492..25712)
                     /gene="coaBC"
                     /locus_tag="N7A07_001305"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU2475998.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25884..26552
                     /gene="radC"
                     /locus_tag="N7A07_001306"
     CDS             25884..26552
                     /gene="radC"
                     /locus_tag="N7A07_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU2475999.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26769..27005
                     /gene="rpmB"
                     /locus_tag="N7A07_001307"
     CDS             26769..27005
                     /gene="rpmB"
                     /locus_tag="N7A07_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU2476000.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27026..27193
                     /gene="rpmG"
                     /locus_tag="N7A07_001308"
     CDS             27026..27193
                     /gene="rpmG"
                     /locus_tag="N7A07_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU2476001.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27291..28100
                     /gene="mutM"
                     /locus_tag="N7A07_001309"
     CDS             27291..28100
                     /gene="mutM"
                     /locus_tag="N7A07_001309"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU2476002.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28139..28618)
                     /gene="coaD"
                     /locus_tag="N7A07_001310"
     CDS             complement(28139..28618)
                     /gene="coaD"
                     /locus_tag="N7A07_001310"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU2476003.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28626..29903)
                     /gene="waaA"
                     /locus_tag="N7A07_001311"
     CDS             complement(28626..29903)
                     /gene="waaA"
                     /locus_tag="N7A07_001311"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU2476004.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30316..31374
                     /gene="rfaQ"
                     /locus_tag="N7A07_001312"
     CDS             30316..31374
                     /gene="rfaQ"
                     /locus_tag="N7A07_001312"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU2476005.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31371..32495
                     /locus_tag="N7A07_001313"
     CDS             31371..32495
                     /locus_tag="N7A07_001313"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU2476006.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32488..33285
                     /gene="rfaP"
                     /locus_tag="N7A07_001314"
     CDS             32488..33285
                     /gene="rfaP"
                     /locus_tag="N7A07_001314"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU2476007.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMNVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            33328..>33662
                     /locus_tag="N7A07_001315"
     CDS             33328..>33662
                     /locus_tag="N7A07_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2476008.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFR"
BASE COUNT         7983 a         8333 c         8933 g         8413 t
ORIGIN      
        1 ccatccgcct gttggggctg gatggttatt cattattttt gtgtcaccga actttcattc
       61 atcaggccag ctcagaaagt gaatcaatgt agtcagcgta ccattgcatc atttcacggc
      121 gaccatcgag atactgggcg tggttatagg tacccctgat ggagttttta tccacatgag
      181 ccaactgcat ttcaatccag gcagaattaa agccttgttc gtgcagaatt gtgctcatgg
      241 tatgcctgaa accgtgccct gtgagtcttc ccttataccc cagcaattca atgacctggt
      301 taatgctctc cttactaatt ggcttgcgag gatcgttcct accgataaag accagaggat
      361 gatgttttga gattggttca agttgcttaa atagcgcaat aacctgctca gagggcggca
      421 caatatgagg ccgtttcatc ttcattactt ctgccgggat ctcccataat tttgcctcaa
      481 aatcaatatc ctcccaatgc gcgaaacgca actcttgcgt tcgtacaccg gttaacataa
      541 tgatctgagt agctgtctta gtgatgatac ttccggtata acccgccaga tcagtaagga
      601 agtggggaag ttcattggca gtaagaaagg gaaaatgtac ttttttcggt gtagctaaag
      661 cactggcaag atcgggagca gggttatagt cggctcgacc agtaacaatt gcatagcgaa
      721 acacctcacc acaacgttgg cggaccttgc gcattttctc cagtgctcca cgtttctcca
      781 tcttacgtag tgcttccaat agctccatcg gcttgatttc ggcaataggt cgcttgccga
      841 tgtaggggaa gatatctttc tcaaaggtgt cgattatttc atcgcggtag cgcagtgacc
      901 agcgatcggc tttcgtctga tgccactcgc gagcaacggc ctcgaaagtg tttttcaaac
      961 tcatttgcag cgctattttt tcttcttttt ttgcctcacc cggatcgcca ccagccgcaa
     1021 gtattttcct ggcttcactg cgtttctcac gtgcgtcggc aagcgttaca tcggggtata
     1081 cgccaataga cagcatcttt tctttaccgg cgaagcggta cttcatacgc cagtatttgg
     1141 aaccattagc gttaatcagc agatacatac ctccgccatc agccagctta tagggccttt
     1201 cttttggctt cgcggttttc actttaacat cggttagcgc catgacatcc tccagctttt
     1261 gatgaatcca tcctcctggg ggaatttccg ccagtaacca acctgatacc cccaatgcac
     1321 ccccacaaac aacttgatat gggttaaagt cgtttgacct caggatacaa agaaaacctg
     1381 atatataagg gattataagg ggctttgttg atgtcgggag agttcaggag aggcaaactg
     1441 gcggaagatc acaggagtcg aacctgcccg ggaccgctgg cggccccaac tggatttgaa
     1501 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
     1561 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
     1621 acacgcagcg tattatcacg aaagcattat aattcatata aaactcatat tttacaatga
     1681 tatatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
     1741 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
     1801 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
     1861 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
     1921 gatgttcttt tataccgata attttggcat acctgccgga tttgtcggaa ccatgttttt
     1981 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
     2041 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgtgt ggcgcactgc catttgggat
     2101 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     2161 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     2221 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     2281 tgtgctggcg accgcgggag gcatgctctc tactgttctg atgatgccac tggttaattt
     2341 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     2401 ggcattcatg atgctggcat tctgtttctt caccactaaa gaacgcgttg aagcaccacc
     2461 tacaacgacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     2521 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtccgcggtg gagcgatgat
     2581 gtattacgtc acatggattt tgggtacgcc ggaagtgttt gtcgcttttc tcaccactta
     2641 ttgcgtgggt aacctgattg gttccgcact ggcaaaaccg ctgaccgact ggaaatgtaa
     2701 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     2761 tgttcctatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtattgca
     2821 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     2881 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     2941 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     3001 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     3061 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     3121 gcacaatctg aaaaccgtta tggaacagct ggcccagggc aaacgccgtt gccagcaaca
     3181 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg acggaaactg
     3241 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     3301 gggtaatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgaa cctggcagct
     3361 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtacg
     3421 gattgagcat tttcaggggg cgctgaataa cggtcctcat tatccgctca atattttgca
     3481 ggacgtgaag gtcacaatcg aaaacacaga acgttacgct gagtttaaaa gtggcaactt
     3541 aagcgcgcgt gtcagcaaag gtgagttctg gtcactggat tttctgcgca acggcgaacg
     3601 tattaccggt agtcaggtga aaaataatgg ctacgtgcag gacacgaata atcaacgaaa
     3661 ttatatgttt gagcggctgg atcttggcgt tggcgaaaca gtttacggtc tgggagagcg
     3721 ctttactgcc ctggtgcgca atggccagac ggtagagacc tggaaccggg acggcggcac
     3781 aagtactgaa caggcgtata aaaatatccc gttctatatg actaaccgtg gctatggggt
     3841 actggtcaat catcctcaat gcgtctcttt tgaagtggga tcggagaaag tctccaaagt
     3901 gcagttcagc gttgagagtg aatatctcga atactttgtt atcgacggcc cgacgccgaa
     3961 agcggtactt gatcgttata cccgttttac tggtcgtccg gcgctgccgc ccgcgtggtc
     4021 cttcggcctg tggctaacca cttcatttac caccaactac gacgaagcga cggtaaacag
     4081 ctttatcgat ggtatggcgg gacgcaatct gccactgcat gtttttcact ttgactgttt
     4141 ctggatgaaa gccttccagt ggtgcgattt tgagtgggac ccgctgactt tcccggaccc
     4201 ggaagggatg atccgccgcc tgaaagcgaa agggctaaaa atctgcgtct ggattaaccc
     4261 ctatatcggg caaaaatccc ctgtctttaa agagttacaa gagaaaggct atttactcaa
     4321 acgcccggac ggttcgttat ggcagtggga taaatggcag ccaggtctgg cgatttatga
     4381 ctttaccaat ccggatgcct gcaaatggta cgccgacaaa ctgaaaggtc tggtcgcgat
     4441 gggcgttgat tgctttaaga ccgactttgg cgaacgtatt ccaatcgatg ttcagtggtt
     4501 tgacggttcc gatccgcaga aaatgcataa ccattatgcg tacatctaca acgaactggt
     4561 gtggaacgtg ctcaaggaca ccgttggcga ggaagaagcc gtcttgtttg ctcgctcggc
     4621 ctccgttggt gcgcagaaat tcccggtaca ctggggtggt gactgttacg ctaactacga
     4681 atcaatggcg gaaagcctgc gcggtggttt gtctattggc ctttcaggtt ttggtttctg
     4741 gagccacgat atcggcggct ttgagaatac cgctctggcg cacgtttaca aacgctggtg
     4801 cgcgtttggt ttgctctcca gccatagccg tttacatggc agcaaatctt atcgtgtgcc
     4861 gtgggcctac gatgatgagt cctgtgatgt ggtgcgcttc ttcacgcaac tgaaatgccg
     4921 catgatgccg tatctgtatc gtgaagctgc tcgtgcgaac gcgcggggta cgccgatgat
     4981 gcgggccatg atgatggagt tcccggacga tccggcttgt gattaccttg accgtcaata
     5041 catgttaggc gacaacgtga tggttgctcc ggtgttcagt gaagcgggcg atgtgcagtt
     5101 ctacttgccg gaaggtcgct ggacacatct gtggcacaac gatgaactcg atggtagtcg
     5161 ctggcataaa cagcagcaca gcttcctgag tctgcccgtt tatgtgcgtg ataacaccct
     5221 actggcgctg ggcaacaacg agcaacgtcc cgattacgcg tggcacgaag gcacggcatt
     5281 ccagctcttt aatctacaag acgggcatga agccatctgt gaagtgcccg ctgctgacgg
     5341 ttccgttctt ttcaccctga aagcggcgcg tactggcaac acgattactg tgactggtgc
     5401 gggcgaggcg aagaactgga cactgtgctt gcgcaatatt gtgaaagtaa atggtctgca
     5461 aggtggttcg caggctgaaa gtgagcaggg gctggtggtg acgcctcaag ggaatgcgct
     5521 gacaattacg ttgtaatcta ttgatggaat gccgggtacg tctgagtcgt acccggcgag
     5581 attgattttt actgcaccgg ctctgtcgat gtaacttcgc tactggaaac gacgcccgca
     5641 ttcatggttt gagtcacttg ctgctttgcg gcgttcacgg catggagttg cccgctaacc
     5701 gtaggtttca gcgggacatt ggcctgaata tcgccactgg cggtaagctg aatattgccg
     5761 tcgccagtca acggtaattc aggccatccc cattgttgca aaatattcac cggcacaccg
     5821 cgtccattca ggctgatatg ggtctgacgt tgtggcgttt gtgaaacact ggcagtggct
     5881 tccagaatgc ctttttcagt aaatgcactc agttcgctga tattcaccgt gctgctgttg
     5941 gcggtcagcg ccagcgacgg gcgacgaaca tcaacacgat tgaatgtcgc ggcggcggca
     6001 ttcagattcg ccgagccact ccagacgccc catttatgat cggtaaccag cgtcaggttg
     6061 gcaccgtaac catcgagcgt ggtgagctgc cacgggaagt cagggtcgat atcaatgatc
     6121 agattgcggc tggcgctaaa tctcttcagt tgcaggctgt ttaaccaacc gggtgtcgtt
     6181 tccatccaca actgttgcca gtttttcggc aaggtatatt ccagcccggc aattgccgca
     6241 tcatcaagga tcaacgtttt cccgtcacgc agccagttcc ctgacgttct gaccataccc
     6301 ccttcccagc ggctggtgaa ctggcgcagc gctacgccct gcggggaaaa ttccgcgttt
     6361 ataatcgggt caaataaatg cagcgaacca taaatgaact cgctagcgtt catcgacagt
     6421 ttgccttctt gtgtctgcca gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg
     6481 aggtcggtca ccgcccagtc cggaccttgc aaacgagcat cgatcacttc caggcgacca
     6541 atctgcaacg acgggacaga gcgtaatggc gcaaagaagt cggttagcga tttttcgctt
     6601 tgtagacgga tatccgccat gcgcagattt tccacttgcc agctgccgtc ggcgttacgc
     6661 tgcgcgtttc cggttaatgt cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg
     6721 cgatcgttat caatactgcc ttcaatcagt acattggtgg caggaacatc attgagcgaa
     6781 agcgatccgg cactaaactg aatctgcgcc ttcgtaccca gcactttacc ggcttctggt
     6841 gaccacggaa ccacgccgcc atttacccgc tgcgcgctca gtttccattc gctattcggg
     6901 ctattaaacg ccatatcacg cagttgcaga cgatcggctt tgaacggtag cggcgcggtc
     6961 tggtcggtga gattcagcgt cccgttttcc agcaggatgg tatcgacatg gcgtggttcg
     7021 gtcagttgcc gactgcttag cgcaatgtcg acactttttg ccaccagggt cgcgggctga
     7081 ccatcacgac caaacgtgac gttctccagc acgatatgag atggcgcgga aaaacggtga
     7141 tccatcgccc cgaaggccag atgatagtcg ctattctcgg aaacccatgc gctgatatgt
     7201 tctgctcccc agcgggtttg cagaagaaaa tagaggccag cgatcaccac taacagagcg
     7261 atgagaatgt agagaagcag cttcccaata aatttcatgg tcttccatcc cgtgaaatgc
     7321 acataaggga gttatgcacg atttacgcgc aatcctcaag gcgggaatgg tgaaagaggt
     7381 gagactgcgg cggtaattat cttaccgccg ccagtgaatt actgtttttc tggtgggaaa
     7441 atcagattca gcacgatggc agtaataccg cccgcggcga tcccggagga gagcaggttt
     7501 ttcagccatt caggggcaaa ctgcaaaatc agcggctgct gagacacgcc cagaccaacc
     7561 gccagcgaca gcgcgataat cagaatcgcc cgacggttca gcggctcacg agaaacgata
     7621 cgcacaccgg aggcggcgat ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt
     7681 tctggaatgt gttgtacaaa accgctcact gccgggaaca gacccagcac gatcaacatc
     7741 agcgcgacga caaaaccgac atagcggctg gcaacaccag tcaactggat cactccgttg
     7801 ttttgcccga agcaggagtt cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg
     7861 ccgtttgcca gcacgccgcc tttcaggcgt ttcatgtaca gcggaccgga cactggctgt
     7921 tcggaaacgt cagaggtcgc cgtgatatcg ccaatggttt ccagcgaagt gatcataaag
     7981 accagcatca gcggcagcag cagactccat tcaatgccaa gaccgtaata gagcggcgtt
     8041 ggcaccataa tcagttcttg cgtcatcggt tcgttgcttt ctggcaacat gcccataaac
     8101 cacgccagcg catatccggc cgccatcgca atcaccagtg aggccacgcg taagtaaggg
     8161 ttacgttgac ggttaagcag gataattaag gctaagacca cgcctgccag cagcagattt
     8221 ttcggtgcgc cgaaggtgtt atcgctcatg gctgcgtaac cgccgccaat ggacgttaac
     8281 ccaacctgaa ttagcgacag gccgataatc atcaccacaa cgccagaaac cagcggcgta
     8341 ataatgcggc gcgccagatg cagaacgcgg gagatcacca tctcggtgca acttgccagc
     8401 atcaacgtgc cgaacaaagc cgccatcatg gtaggaacat cagcaccacc ggttttcagc
     8461 gcggtaccgc ccataatcag cggggcaaca aagttgaagc tggtgccctg aatagacaac
     8521 agcccggagc caaccggacc ccaggcctta atttgaataa tcgatgccac accggaggca
     8581 aacagcgaca tactaataat gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag
     8641 attaatagcg ctggcgtgat caccgcaacg aacatcgcca gcagatgctg acaggcggca
     8701 aacagggttt gaggaagcgg cggacgatct tcaagacggt aaatcagttc gctgttttga
     8761 gtctgcgcaa ccggttgcgc attttctgac tcgagggtgg aaacagacat cgaaaacaat
     8821 cccgtggtag caaagcgggc attttagctg accgaaagag aaaagcaaac gtttgccaca
     8881 tcctcatcta aatatttaaa tgagataaat gcactttttt atataacttt tggtattttt
     8941 ctgcttaaaa tccatgccat ttgttcatta acagctgaga atttgaccca ggaaagcata
     9001 actcgatacc cgtcatagtt catgaagttg ccactgcgcg atgaagtatg acgagtatga
     9061 aagagtgatg cggatacaaa ggagtaacta tgtttcatct cgatacttta gcaacgcttg
     9121 ttgccgcaac gctgacgttg ttgctcgggc gtaagttggt ccattccgtc tcctttttga
     9181 agaaatacac cataccggaa cctgttgcgg gtggtttgtt ggtggcgctg gcgctactag
     9241 tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc gatccgttaa
     9301 tgctggcttt cttcgccacc attggcctga acgccaacat tgccagtttg cgtgccggtg
     9361 ggcgtgtggt tggcatcttc ttgattgtgg ttgttggtct gttggtgatg caaaatgcca
     9421 ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg ttggccggtt
     9481 ctattacgct ttccggcggt cacggtacgg gcgctgcgtg gagtaaattg ttcattgaac
     9541 gttatggctt caccaatgcg acggaagtgg cgatggcctg tgcaacgttc ggtctggtgc
     9601 tgggcggctt gattggcggt ccggtggcgc gctatctggt gaaacactcc accacgccga
     9661 acggtattcc ggatgaccag gaagtcccga cggcgtttga aaagccggat gtgggccgca
     9721 tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc ctgacggtgg
     9781 ggaaaattgt tgcgcaactt ttggctggca ctgcttttga actgccgacc ttcgtctgtg
     9841 tactgtttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt taccgcgtct
     9901 ttgagcgtgc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg gcgatggcgc
     9961 tgatggggct gaaactgtgg gagctggctt cgctggcgct gccgatgctg gcgattctgg
    10021 tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc atgatgggca
    10081 aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc ggtgcaacgc
    10141 caacggcaat cgccaacatg caggcgatca ctgaacgctt tggcccgtcg cacatggcgt
    10201 ttttggtggt gccgatggtc ggtgcgttct ttatcgatat cgtcaatgcg ctggtgatta
    10261 agctgtattt aatgttgccg atttttgccg gttaaaaatg aaaaagcgtc ggaaggaacc
    10321 ggaaacgcta cgtgaacact gtcgccatat ttttggtgat gagccgccag tgctctgtgt
    10381 atgggaaacg gaatttgatt acgcagatgc tgaactcaag gcgctggcgg ctaaagagtg
    10441 gcagcagatc agcgaatggg atttgagtgc ttactatgta ctcaatctcg tttataacga
    10501 accgatgcaa atagagttgt ttcgctatct gtttccgctc tgcctggcgc aatggcatga
    10561 aaccgtttta gctggcggtt atggtgatca cttcgaagaa tccttaatga aggcattgtg
    10621 tcggccctat ctctggcagg agatgatgaa tgcttcccaa cgccagcagg tacggcaatt
    10681 tttactggac accgcgttgc agcgtatgga taacgagcgt ggtttcaata acgttctttg
    10741 ctggctggcg gtgttcaata ctctgggtgg ggccgcgccg ttaattcgtt ctctctggtc
    10801 gcgctggtgg gcgctggata cgccaggaaa agcggtgtgt gctattcagt atgccgcgca
    10861 tctaatttat cccatagaag ccaatccgtt atggtcgcaa gaatggatag gctgggggca
    10921 tccattaggc cataaagatg gctggtcctc tgataatcgc gcttttcttc gccaaatgtt
    10981 aacacctgaa atgattgtgg ctggcgtaca ggcagcggct gaaattctgc gtggtgaacc
    11041 ggaaggcgca atggcggcgc gaattgcaca ggatgcgtat gaggcaatgg atatcctcac
    11101 tatccagatt gaagatttac tgcgtgactt gtcttgtgat gagtctggtc atgcgttgga
    11161 gtaacgttcc gtctccggca tccagcgttc tatcagggct tttgcttgtt gtgggtaacg
    11221 ttcgtgaata tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat cgcgcagtaa
    11281 atccgccact ttaaactcag cattacccgt ctgacgcgta cctaacagtt cacccggtcc
    11341 gcgaatctct aagtctttct gtgcaatcac aaaaccgtcg ttgctgtcgc gcagcacttg
    11401 cagccgaatt tgcgcggttt tggagagcgg cgttttgtag agcagcacgc agtgagaagc
    11461 caccgcacca cgacctacac gcccgcgaag ctggtgtaac tgcgccagac ccagacgctc
    11521 agggttttcg ataatcatca ggctggcgtt aggcacatca acaccgactt caataacggt
    11581 agtggcaact agcagatgta gctcaccttg tttaaacgac gccatcaccg cctgtttctc
    11641 ggctggtttc atccgcccgt gtaccaggcc aacattcaat tctggtagcg ccagtttcaa
    11701 ctcttcccag gtagcttccg ccgcctgcgc ttccagtaac tctgactctt caatcaacgt
    11761 acaaacccag tacgcctgac gaccttcagt tatgcaggcg tggcgcacgc ggtcaatgat
    11821 gtcggtacgg cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc ctggcggcag
    11881 ctcatctatc accgaggtat cgagatcggc atacgccgtc attgccagcg tgcgggggat
    11941 cggcgtggcg gtcatgatca actgatgcgg atggaagcct tgctgctggc ctttctccca
    12001 taacgccaga cgctgatgca cgccaaagcg atgttgttcg tcgataatca ccagcgccag
    12061 accgttaaac tgcacctgtt cctggaagat ggcgtgcgta ccaacaatca tctgcacctg
    12121 accgctggcg atggcttcct gctgagacag tcgcgctttg cctttctgct taccggcgag
    12181 ccagcccact tcgataccga gcggttcaaa ccagttgcgg aagttattgg cgtgctgttc
    12241 ggcgagtaat tcggttggtg ccatcaatgc cacctgtttg ccgtgggcaa tcgcacgcag
    12301 cgcggcgagg gcggcgacca gcgttttacc ggaacctaca tcgccctgta ccaggcgcat
    12361 catcggcaca tccagcgcca tatcgcgctc gatctccgcc actacgcgtg cctgtgcgcc
    12421 cgttggcttg aacggtaagg ctgcgaggag tttatttttc agcgcatcat tggcgctcag
    12481 cggctgggca tgaaaacgct gtgctccggc acgtaaggct aacatgctga ggttgtgcgc
    12541 cagcagttct tccagaatca gacgacgttg cgctggatgc tgcccggttt ccagatcgct
    12601 aagctgtagc gtcggtggcg ggcggtgcaa agtgcgcaac gcttccggta gcgtcatcat
    12661 tccttgtgac agttccggcg gcaggagttc ttcaatggcg caggtgtcga gcagatccag
    12721 cgcctggtcg gttaatttac gcagcgtggc ctgctttacg ccttccgttg ttggataaac
    12781 cggcgtgagc gtttcctgta attctggcgt gctgagatcg ccctgcacgc ggtattccgg
    12841 gtggatcatc tccgcaccat atttaccgcg ctttgcttcg ccataagcca gtacacggcg
    12901 gcccgtcgcc aggctatttt tcattgccgc gctgaaattg aaaaagcgca tggtgaggat
    12961 gccggaaccg tcgctgatct ggcaggtcat catccgccga ccgccgaagg agatattgca
    13021 gttcagcact tcgccttcca ccgtggcata aacgcccggc agtagttctc cgatggggta
    13081 gagatgggtg cgatcttcgt agcgcagggg aaggtgtaag agtagatcct gcacggtatg
    13141 caggttgatt ttcgccagtt tgttactaag tgctgcgcca acgcccgtta gggaactgag
    13201 tgggacagca tctaacaggc gacctgtcat ggcacttacc ctgcagcctg catagtagcc
    13261 caccagtcgg catcagcttc gatctcgcct tgctgattga cgtggggata aggcaggcct
    13321 ttgcgttttg cgactttcgc cagcaccgga tagccgcctt caaacaacag gcgttgttgc
    13381 tctgcttccg gcaacatgct gttttcacgc aggtacatgc ctgcattttg ccgctgacgc
    13441 tgggcttcgt aaagaatgag ggctgaggca acggaaacat tcagcgactg caccatgccg
    13501 atcatcggaa tgatgatgtc ctgatccgcc agggccaatg cttcctgcgt gatgcccgtt
    13561 ttctcctgtc ccatcaaaat gcaggtcggg cgagtgtaat caatttcgcg gaaatcgaca
    13621 gcgttatcag aaagatgggt tgccagaatc tgcatgccct ggcctttgag atgagcgacg
    13681 gcatcgccaa tggtgcggtg tgttttcacc tgtacccagc tgttactacc cgccgctgcc
    13741 gaagccatgg tgcgcatgcg gctaccaggc cagacggcgt gaacttcatg tacgccaacg
    13801 gcatctgcgg tacgaataat cgcagaaacg ttatgaggtt tgtggacctg ctccatgcag
    13861 acggtcagat caggctgccg cctggcgagc atttcgcaga tgcgtgcata acgtgttggg
    13921 ttcataaaac attaatttcg gtttcgggtg actttaatca cgtctggcat cacgcggatt
    13981 ttgcgcatga tattcgccag atgcacacgg tcacgagcgg tcagacgaat aaaggcgctg
    14041 tagacgcgac catctttctc ttccgtattc aaactttgaa tattcgaggt cgtggtgtta
    14101 attgccgccg tcaggtttgc cagcgcaccc tgatgattga acatctccac cttgatttcg
    14161 gtgatgaact cctgcgccgt ctctttatcc cattccacag ccataaactt ctctggctct
    14221 ttctggtagc cacggatgtt acggcaggat tcatggtgga tcaccagacc tttaccgggg
    14281 ctgacgtggg cgataatcgg gtcgccagga atggggcggc agcatttcgc aaaggtgatc
    14341 agcacgccat cagcgccttt gatcggcaga tgcccgtggc tttgagtcgc cggtggaatg
    14401 gaggcgtccc catgttgtag atttttcgcg accaccacgc tcattgcgtt accaaggccg
    14461 atttctgcca gcagatcgtc aagcgttgcc agcttcatgc gatccagctc gcgctgaata
    14521 ttttcctgcg ggatttcatt gagcttacgg ctaccaccca aagcatggtt gagcagacga
    14581 cggcccaggc ttacagaatc atcacgcttg aggtttttca gcaactgacg aattttggcg
    14641 cgcgctttcg agctaacgac aaagttcagc caagcggcat tcgggcgagc gcccggagcg
    14701 gtaatgattt caacggtctg accgctagta agcggctgcg acagcgggta aggctggcgg
    14761 tcgacgcgtg cgcccacgca ggcatggccg atatcggtat gcactgcata agcgaagtcg
    14821 acgggcgttg caccggcagg cagctcgaca atgcgccctt ccggtgtgaa aacgtaaatc
    14881 tcatccggga agagatcgga tttaacgctc tcgataaatt caaacgaact accggcgctc
    14941 tgttgcagct ccagcaggct ttgcatccag cgctgggcgc ggatttgtgc ggtagtactg
    15001 gtttcgccgt gctctttata agcccagtgc gcggcaacac ccatctccgc catctgatcc
    15061 atatcttcgg tacggatctg gacctcaacc ggcacgccgt gcgggccaat catcgaggtg
    15121 tgcaacgact gatagccgtt cgcttttgga atggcgatat agtctttcac gcggcccgga
    15181 cgcggcttgt acaggctgtg catctggccc agcacgcgat aacaggtgtc agaatcattg
    15241 acgatcacgc ggaaagcgta gatgtccatg attgagtgaa aacgctgctc tttgagcacc
    15301 attttacagt aaatcgaata aagatgcttt tcgcgaccac tgacgcggca cggtattccc
    15361 gcttcctgca aacgcccttc gatttcagaa agaatcttct ggatcatctc tttacggttg
    15421 ccgcgcgcgg ctttcaccac ttctttgatt acgcgataac ggttgggata cagcgcctca
    15481 aaacccagct cttcgagttc ggttttaatg tggtggatac ctaaacggtg cgccagcggg
    15541 ctgtaaattt cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg aagtgagccc
    15601 agcgtgcgca tgttgtgggt acggtcggca agtttgatga ggatgacgcg gatatcctgc
    15661 accatcgcca taatcatctt gcgaaagttt tcggcctgcg cctctttctt atcgcggaac
    15721 ttgagtttat caagtttcga caccccctct accagctcgg cgacgctttt accaaaaagc
    15781 tgttccatat cctggtaggt ggcgggagta tcttcaatca cgtcatgcag cagcgccgcc
    15841 atcagcgttt catagtcgag tttcatctcg gccagaatgc aggcaaccgc taccgggtgc
    15901 gtgatatagg gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg tgcaacgaga
    15961 tacgcctgcc gcagacgctt gatttggtct tccggcaggt aggtttgaat cagttgattc
    16021 aggctttcaa acagatacaa gggcgacccg ctttgtgatt aacgacgacc ttcagcaata
    16081 gcggtaacgg cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg
    16141 tcgaggatct ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt
    16201 ttatcgtttt cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga
    16261 cgcgcggcga ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca
    16321 gttacgcgtg ccatacttaa aaagctccac aggtgaagaa atgactgggc atgatactga
    16381 aatcaggttc agtctgccaa caatttgctg attaaagcgt catgacgctg cttttggcgg
    16441 ctcatgcgca gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc ggtatcgaag
    16501 tcatcattca caatcaggta atcatattcg gcgtaatggc tcatttctgc aacagcttgc
    16561 gccatacgct ttgcaatgac ctcttcgctg tcctgatcgc gaccgcgtag acggcggtcc
    16621 agttcaattt tagacggcgg taaaataaag atactccgcg cgtgcggcat cttctggcga
    16681 atttgctgcg cgccctgcca gtcgatatcg agaaaaacat cgacaccggt cgccagtact
    16741 tgctcaatgg cctcacgcga agtgccatag taattaccaa aaacttctgc gtgttcgagg
    16801 aacgcatctc tgctaatcat ttctttaaat tcatcatgat taacaaagaa ataatgttca
    16861 ccgtggactt caccaggacg cggttggcgt gtggtgtgtg aaacagaaac ctgggtgtca
    16921 tacaacggtt gggtttttaa taaagcctga atcaggctgg atttacccgc gccactgggg
    16981 gcagaaacaa tataaagcgt gccttgagcc atgagtatct ttcgtatgtg attagcgaaa
    17041 taaagcctac atacgagctt attatacacg gcgcggccat gtgacgtagc ccttgctcac
    17101 actttttgcg caaagttgtt gcgttttgcg tagctgtttc cgcatttgcc tgtgatttac
    17161 tgcaataaag acgattctca ggaagcgtcc tcgcagactc cgtttttgcc actcggcttt
    17221 gccttcacca cgttgtatat ctgccagcct gactacagcg gaggtgaaga tgaaagtatg
    17281 gatggcgata ttaataagta tcttgtgctg gcaatcatct gtgtgggcgg tctgtccggc
    17341 ctggtcgcca gccagagcac aggaagaaat tttccgcctg caacagcaaa taaaacagtg
    17401 ggacgatgac tactggaagg aaggaaaaag tgaggtggaa gacggtgttt acgatcagtt
    17461 aagcgcccgt cttacgcagt ggcaacgctg ctttgtgagc gagccccgcg acgtgatgat
    17521 gccgccttta aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggc
    17581 ggataaaaac gcattaagtc tgtggatgcg agaacgtagc gatctttggg tgcagccaaa
    17641 agttgatggc gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag
    17701 tcgcggtaat ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa tttccgctgt
    17761 gccgcaaacc gttagcgggc ctttagccaa cagtacgctt cagggggaaa tatttctcca
    17821 gcgcgagggg catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg
    17881 cctgatgatg cgccaggacg atagcgacac gctgaattct ctgggcgttt ttgtctgggc
    17941 atggccggat ggaccgcagt taatgaccga tcgtttaaag gagctggcta ccgcaggttt
    18001 tactctaacg cagaggtata cccgtgcggt taaaaatgct gatgaggttg cgcgcgtacg
    18061 caatgagtgg tggaaagcga aattaccctt cgtcaccgat ggcgtggttg tgcgaggggc
    18121 gaaagaaccc gaatcccgcc attggctacc aggccaggca gaatggctgg tggcctggaa
    18181 atatcaacct gtagctcagg ttgccaaagt gaaggcgatt cagtttgcgg tgggtaagag
    18241 cggtaaaata tcggtggttg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca
    18301 gcgggtgaat attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca
    18361 gatcctcgtc agccttgccg gtcagggtat tcctcgcatt gatgatgtgg tctggcgcgg
    18421 tgcagaacgt acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt
    18481 tgcttctgat gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca
    18541 ggttctgggg ctggatggca ttggtgaggc cggctggcgc gcgctgcatc agactcatcg
    18601 ctttgagcat atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg
    18661 gatcgcgaaa agtaaaagtg cgcagctatg gcatcggttt aatctggctc gtaagcagcc
    18721 ttttactcgc tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag
    18781 tgatgaacgg tcctggtcgc aacttttatt tagcacggag cagttctggc agcagctgcc
    18841 ggggactgga tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa
    18901 gaagctgggc agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgtccttc
    18961 gtggctgtag taaaagaccg gtaatcccag cttaaggcgt agcgccagta agcgggcaaa
    19021 aaagccgaat accagggtgg agataattac cacatcatgg ttagaaacat agtgttgcag
    19081 cgcgatgtac agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac
    19141 cagtgggata cgtttacaga acatatcgcg caaaacgccg ccaaacacgc cggtggttac
    19201 cgccgcgaca acggcaataa ttgggccgtg ccccatatcc agtgcaacct gtgcgccgat
    19261 gatagaaaag accaccagtc caagcgcatc gagcaccaga aacactttgc gcaggtaagg
    19321 cattacagga gcaacgatag tggtgagtac tgcggcggta gcgacgataa tcacatattc
    19381 cgggtgtttg acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc
    19441 gccaattgcg gtggcggtcg caataataat tacgccaaat gtatccatgc ggcgtcgccc
    19501 ggccgccaga gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa
    19561 cagcatggtt ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac
    19621 gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt
    19681 ttctgtagcg tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata
    19741 ggttaaaatt gaggggtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc
    19801 tggactttgg cttacgcatc gggtatctca atcaacaaaa agagttttta gtttctgcta
    19861 tttcttctct gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg
    19921 caactcctca ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgctacccac
    19981 atcaaagtgt gtggtattag cttgctgttt tgaattcacg ccatcccgct tgagtttttc
    20041 ttctgtttgg gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag
    20101 gatcttctga ctctttttca gccccttcag ttggtggatg gctttctgat ctaatccacc
    20161 atacagcccc tggtaaccat gattctgaaa tatggcgaaa tcagtagctg tcgctaccgc
    20221 agcttgctgt gctgcctcaa ccaattgttt gttatgttct ttcaactcat ttctcagaaa
    20281 cagacgcttt tcgtcttcac gaagttgctt aaatgcttca tcgtcagcaa gctcctgccg
    20341 tcgggtctgg atagcaaaat aagtctgccc tgccgcaatg accggtttcg cagggtcgcc
    20401 gttttgtact accaggtaac aggcatagcg ggagagatga acatcttcaa gttctcgttg
    20461 agcccctgaa cctaatacaa ccattttggt ggtttcaacg aaatggtcag atgcagcttg
    20521 attgctggct tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat tgcgccagtc
    20581 ccgataatcc agtaacggtg ccaattctct agctgaccaa aattcttgcc cttcagtgcc
    20641 ataatgccgt atttcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata
    20701 tcgattgcaa agaaagtaaa tctgtaactg tatttatata cagttgtgag tatctcacgt
    20761 tctcagtgtg ctgggcaaaa aaacatcaca aaatctggag caggcacgca aaacagaatt
    20821 gaagttgcgg ggaagaaaaa tgccgctctg tttgagtggg cggcatttgt gaaggctgaa
    20881 aagtaagaat tggcgttacg agttattcaa tgttctgaat ctgctcgcgc atctgctcaa
    20941 tcaacacttt cagctcaatc gcggagtttg tcacttcggc attgatagac ttcgacgcaa
    21001 gagtgttcga ctcgcggttg aattcctgca tcataaagtc cagacgacga ccaacggctt
    21061 cttttttctt cagaatgttg taggtctctt tgacatgcgc ttccaggcgg tccagttctt
    21121 cggcaacgtc aattcgttgt gccagcagaa ccagctcctg ctccagacgg ttgttttcca
    21181 actgaacctg agcatcttcc agcttcgcga ccagacgctc acgctgccat tgcaggattt
    21241 ccggcatatg ggcgcggact ttgaccactt cggcggtgac gccttccaga cgctgctcga
    21301 tcaatgcttt cagtgcctga ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac
    21361 catccagcgc cgcgagaatt tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca
    21421 tcacgcccgg ccagcgtaga atatcaaccg ggttgatttc cccttcgtca ctctgcattt
    21481 ttacccagtt cgcggcagtt accagctgtt tagccagttt ttcgttgagg atcagctcac
    21541 cttgcgcgct aacatctggc tcatagcgca gggtacattc cactttaccg cgcgtcaggc
    21601 gagaacgaat acgctcgcga acgacaggtt caaggctacg gaactgctcc ggcagacgaa
    21661 agtaagtttc cagataacgc tggtttaccg agcgcatttc ccaggttgcg ctcccccatt
    21721 cacccttgat ttcacgccgg gcgtaggcgg tcatactgcg gatcatagac gttcctgttt
    21781 ataaaaggag aggtggaagg attatagcca tcgatgcctt gtcaggatag gaataaccgc
    21841 cggaagtccg tataatgcgc agccacattt gtttcaagcc ggagatttca atatgcgtcc
    21901 agcaggccgt agcaataatc aggtgcgtcc cgttaccctg actcgtaact atacaaaaca
    21961 tgcagaaggc tcggtgctgg tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat
    22021 tgaagaaggc gtgccgcgct tcctgaaagg tcagggccag ggctggatca ccgcagagta
    22081 cggcatgctg ccacgttcta cccacacccg taacgctcgt gaagcggcga aaggtaagca
    22141 gggtggacgc acaatggaaa tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga
    22201 tttgaaagcg ctgggtgagt tcaccattac gctggactgc gacgtgcttc aggctgatgg
    22261 tggcacgcgt accgcgtcga ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa
    22321 gctggtggaa aacggcaagc tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc
    22381 tgtcggaatt gtgaacggcg aagcggtttg cgatctggaa tacgttgaag actctgccgc
    22441 agagaccgac atgaacgtag tgatgaccga agacgggcgc atcattgaag tgcaggggac
    22501 ggcagaaggc gagccgttca cccatgaaga gctactcacc ttgttggctc tggcccgagg
    22561 gggaatcgaa tccattgtag cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg
    22621 actgatgagt cgcctttttt ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa
    22681 ccatatcagc gccagtttat tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag
    22741 tttacgttga agtccgggcg caaaagcccc tatttcttca acgccgggct gtttaatacc
    22801 gggcgcgatc tggcactgtt aggccgtttt tacgctgaag cgttggtgga ttccggcctt
    22861 gagttcgatc tgctgtttgg ccctgcttac aaagggatcc cgattgccac cactaccgcc
    22921 gtggcgctgg cggagcatca tgaccttgac ctgccgtact gctttaaccg caaagaggca
    22981 aaagaccacg gtgaaggcgg caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg
    23041 gtagatgatg tgatcaccgc cggaacggcg attcgcgaat cgatggagat tattcaggct
    23101 aatggcgcga cgcttgctgg cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc
    23161 gagatttcgg cgattcagga agttgagcgt gattacaact gcaaagtgat ctctatcatc
    23221 accctgaaag acctgattgc ttacctggaa gagaagccgg aaatggcgga acatctggcg
    23281 gcggttaagg cctatcgcga agagtttggc gtttaaagaa actcgccgga tgaaaagtca
    23341 tccggcgtca tattactgca actgtgccgc aattagcggc cagcgggcgt caaaatcatc
    23401 cgtcgggcgg tatttaaatt cgctgcggac aaaacgtgac agcatacctt cacagaaggc
    23461 taggatctgg cttgccagca gggtttcatc ggtggtgtaa ccttcaccct cacgcattct
    23521 cttttcacgc aatacctgac gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg
    23581 cccttgcagg cgatcctgtt caaacattag cgcatgacca gtgaggatgc gggtcaggcc
    23641 aggattacgc tcaccaaaac cgagaagcag caacacaatc agacgcaggc gcgctgtggt
    23701 gtctttctca tctttcagaa tcagattgat gcgagtaatc aggctatctt cgataaactc
    23761 aatcaggcta tcgaacatgc gggtcttact ggggaagtgg cgatacagtg ccgcttcgga
    23821 aacgccgaca gaggcggcca gttttgccgt cgtgatacgt tggcttccat cgctggattc
    23881 cagcatcagc gccagagact gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt
    23941 ttctgccatg ttacaaaata cccctgaaaa taagcacttg ccaggcggca cccacgctat
    24001 gaccgcaaac gaaatgttta cggctatgtt atgacgttat tcggatgcgt atgtgttact
    24061 gacgaccaga gtgaccaaag ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca
    24121 gattaaattc agcctgtact accggaacaa aaatcatctg ggcgatacgt tcgccaggtt
    24181 gaatggtgaa gctgtcctga ccacggttcc acacggaaat catcaactgg ccctgatagt
    24241 cggaatcgat caatcctacc aggttaccaa gcacgatacc gtgcttatgt cccaatccgg
    24301 agcgcggcag catcattgcc gccagtgaag gatcggcaat atgaatcgcc agcccggtcg
    24361 gaaccagcgt agtgtcaccc ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt
    24421 caagtccggc agagccagag gtggcataag tcgggagcgg aaattccttc ccaacgcgcg
    24481 ggtccagaat cttaacgtcg atttttttca tcataacggg tcacgatctc gtcgagtaat
    24541 aattggccaa ggagctcttt gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa
    24601 aggtgtaatg cgttgttgtc gctgttaaat ccttgagttg gctgggaaac atcgttcgcg
    24661 cagatcagat caaggttttt acggatacgt ttttgccggg cgtattcttc cacattattt
    24721 gtttcggcgg caaatccaac gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg
    24781 acgatatcgg ggtttttaac catttttatt gttaattcat caccctgcgt ggcctgcttt
    24841 ttgattttct ctggggccac ggtagctgcg cgataatccg ccacggcggc gcagccgata
    24901 aaaatatttt gctgctgtac agaagcattc acggcggctt ccatttccag cgcggtcatc
    24961 acatcaacac gtttaacaaa cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg
    25021 acgttcgcgc cacgacgggc agcggcggcg gcgatagcaa aacccatctt gccggagctg
    25081 tgattagaga tataacgcac gggatcgagc ggttcacgcg tcgggccggc ggtaatcata
    25141 atgttcagat gtttcaggtc gttgacgggc gaaaaatgcg ctaccgccat atccacaatg
    25201 gttaacggat cgagcattcg cccaggaccg atatcaccac aagcctgact gccactgtct
    25261 ggcccccaga tgagcaaacc acgggaagca agcacctcta aattatgctg cgtggcagcg
    25321 gcacggtaca tctgctggtt catggcgggg agcacggcta caggcgcagg tgtagccaga
    25381 caaatcgtcg ataccaggtc attcgccatt ccggcagcaa cacgggcaat caaatctgcc
    25441 gtggcagggg cgagaatcac taaatcagcc catttaccca gctcaatatg gcccatagcg
    25501 gcttcggctg ccgggtccag caaactgtcg gaaacgggat aaccagaaac cgcctgcaag
    25561 ctaagtgggg tgataaaggc ttttgccgct tcggtcatgg ctacgcggac gtcggccccg
    25621 cgatcgcgca aacgacgcac cagttcaggg gttttatagg cagcaatacc gccgctaacg
    25681 ccgagaacga tttttttacc ggccaggctc atgatgattt ttcctgttgg gtgacaccag
    25741 aagttggcga ttttatcaca atacttttgt tgtcgtgcct tcacctgaga ttcactttgc
    25801 gaggcgcttt ccaggattga aaactggccg tcgatttaac ggaacggcta tgacaggatg
    25861 cgagcaccac aaaggaggtg aaggtgaaaa acaatgcaca gctgttgatg ccgcgcgaaa
    25921 aaatgctgaa gtttggtatt agcgccttaa ctgatgtcga gctgctggcg ctatttctgc
    25981 gtaccggaac gcgcggtaaa gatgtattaa ccctggcaaa agagatgctg gagaatttcg
    26041 gctctcttta tggcttgtta acctctgaat atgaacaatt tagtggtgtt catggaattg
    26101 gcgtagcgaa atttgcccag ttaaagggga ttgctgaact ggcgcgacgt tactacaacg
    26161 tgcggatgcg tgaagaaagc cctttactca gcccggagat gacgcgggaa tttttacaaa
    26221 gccagctcac gggtgaggag cgggagatct ttatggtgat ctttctcgac tcccaacacc
    26281 gggttataac gcatagccgt cttttttccg gcacgctaaa ccatgttgaa gtccatcctc
    26341 gggaaattat ccgcgaagcg ataaaaataa acgcctcggc gctgatcctt gcacataatc
    26401 acccttcggg ttgtgctgaa cccagtaaag cggataaact cattactgaa cggataataa
    26461 agagttgtca gttcatggat ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt
    26521 atgtttcttt tgccgaacgc ggctggattt aacccgctat gcgcgatcct tcgggatctt
    26581 tgtctgttcg ggacttgagc acatcgctga gtcagcgtat actacgccac ctttgagaat
    26641 ctcgggtttg gcatttgggc ctggcaatcg agagttcaca tagaactgcg atgaccgggc
    26701 tgtaaagcct gacgaggcgc caatacccca tacgaagctc gagctaattt gatttttgga
    26761 gaatagacat gtcccgagtc tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc
    26821 gttcccacgc actgaacgcg actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt
    26881 tctgggttga gagcgagaag cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg
    26941 taatcgataa aaaaggcatc gatacagttc tggctgaact gcgtgcccgt ggcgaaaagt
    27001 actaagtact tagaggaaat aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt
    27061 ttcttctgct ggtactggtc acttctatac cactacgaag aacaaacgta ctaagccgga
    27121 aaaactggaa ctgaaaaaat tcgatccagt tgttcgccag cacgtgatct acaaagaagc
    27181 gaaaatcaaa taattctcgc tttgatgtaa caaaaaacct cgctccggcg gggttttttg
    27241 ttatctgctt gcccccatat tgactgcatc tgttcattcc tggagatgct atgcctgaat
    27301 tacccgaagt tgaaaccagc cgccgcggca tagaaccgca tctcgttggt gcaaccattc
    27361 ttcatgcagt ggtgcgcaac ggacgcttgc gctggccggt ttcagaagag atctaccgtt
    27421 taagcgacca accagtgctt agcgtgcagc ggcgggctaa atatctgctg ctggagctgc
    27481 ctgagggctg gattatcatt catttaggga tgtctggcag cctgcgcatc cttccagaag
    27541 aacttccccc tgaaaagcat gaccatgtgg atttggtgat gagcaacggc aaagtgctgc
    27601 gctacaccga tccgcgccgc tttggtgcct ggctgtggac caaagagctg gaagggcata
    27661 atgtgctggc ccatcttgga ccggagccgc ttagcgacga tttcaatggt gagtatctgc
    27721 atcagaagtg cgcgaagaaa aaaacggcga ttaaaccgtg gctgatggat aacaagctgg
    27781 tggtaggggt agggaatatc tatgccagcg aatcactgtt tgcggcgggg atccatccgg
    27841 atcggctggc gtcatcactg tcgctggcag agtgtgaatt gttagctcgg gtgattaaag
    27901 cggtgttgct gcgttcgatt gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg
    27961 atggtaaacc gggctatttc gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt
    28021 gtcgggtgtg cggtacgccg attgtggcga ctaaacatgc gcagcgggca acgttttatt
    28081 gtcggcagtg ccagaagtaa ttcatgcgcg ccggatggca taccatccgg cataaacgct
    28141 acgctaactt cgccatcagc gcctgatgga cattctccgg caggaaatgg gtgacatcgc
    28201 cctgatggcg cgccacctct ttcaccaacg atgaagagat aaacgaccac tctttcgacg
    28261 gcatcagaaa cacactttcc agttccggca ttaagtggcg attcatatgc gccagctgca
    28321 tttcatattc aaaatctgcc accgcacgca ggccacgaat cagcaccgta gcgtgttgat
    28381 tacgggcgaa gttcgccatc aaatcactaa acccgaccac ttccacgttc cccagatgcg
    28441 cggttgcctg ctgtgccagt gccacacgct cttccagggt aaacatcggt tttttactgg
    28501 ggctggcggc aatcgccaga ataacgtgat cgaacatctg cgtggcgcgc gtcacgatat
    28561 cgatatgacc attggtaatg ggatcgaaag tacccggata aatcgcccgt ttttgcataa
    28621 caacctcaat gcgttttcgg tggcaggtaa ggttccagca gttgaagcag acgctgtagc
    28681 gcgccctggt tttgatacag tacttcaacg gcatgacggc catagaaact acggtaatcg
    28741 gcgtcggtga gtaaagagga aacctctttt gcaagcgtag tggcatcggt aacggtaatc
    28801 agcccgcttg cctgctccag ccgcgcgcaa atgtctttaa agttaaaagt atgcggcccc
    28861 atcaataccg gaattgcgtg tgcggcagct tccagcggat tatgcccacc acgttcaacc
    28921 agtgaaccgc caacaaaggc gagatcggca atgccgtaca gtaacatcaa ctcgcccatc
    28981 gtatcgccaa ccacaacctg cgtgctggtg gaggggactt cccctgaaga gcgtgtgata
    29041 tagcttagtc cagcctggcg gacaaggtta atcgcatccg ggaagcgttc tggatgacgg
    29101 ggtaccagga tgagcaataa attcgggaat tgctgtaata aagcctgatg tgcggcaatg
    29161 accacgctct cttcaccttc gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc
    29221 cactggcggc gcagcgtcac ggctttagca gccaactgcg gcgttacaga aatatcgaat
    29281 ttcaggctac cggtaacggt tacctggtta tttttcgcgc ccagcgccac aaaacgtgca
    29341 ccatcttctt cattttgggc ggcaatcagc gtaatacgac gcagcaagcg acggacgaat
    29401 ttacccagtt tggcataacc tgcggccgag cgggcagaga gtcgcgcgtt agcgatcacc
    29461 agcggaattt tacgtttatg tagcgccgca atcaggttag gccatagttc ggtttccata
    29521 atcaacacca gtttagggtc gactttattc aggaaacggt tgagtgcatc gggcagatca
    29581 tacggcagat aaacgtgctg aacatccttc ccgaaagccg attgtacgcg ctccgaaccg
    29641 gttggcgtca tggttgttac ggtaataggt aaatcaggat aacgatgacg cagcgcgcgc
    29701 accaacggga tcgccgccag agtttcaccg acggagacgg agtgcagcat aatgccgcct
    29761 ggttttagcg gatggcggta aaaaccgtaa cgttcacccc agcgttttcg ataggccgga
    29821 gccttacgtc cgcgcaccca gagccgtatc cagatcagcg gctgaataag gtagagaagg
    29881 gcggtgtaaa gcaattcgag catagtaaat agctgactta tggatgtgct ggggattcta
    29941 tgtatttagc tgtggcttta ccattacttt tcccgttttt gacttaaata gcaatttacc
    30001 cgccaattct tgctgaacat ctatactttt tttgtatttt tatgcgattc attgatttct
    30061 gctgtacgtg caggaaggaa tcgcagagca acagtgaaag aatatggttg ctaatgattt
    30121 tatttgattg atttctttat tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat
    30181 tgccgcgaaa gaagtgatac actagcgcgt caatatcagt gcggtatcag tattcaggta
    30241 gctgttgagc ctggggcggt agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa
    30301 caaaaagagt tacttgtgga taagccattt cgaagaattt tgctcattaa gatgcgtttt
    30361 catggggata tgttattaac tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac
    30421 gcaaaaatcg atgtgctgct ttatcaggac accatcccga tcctgtctga aaatccagag
    30481 attaacgcgc tctacggcat aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac
    30541 ttttttcatc tcatcaaggt attacgtgcc aataagtatg accttatcgt caatcttacc
    30601 gatcaatgga tggttgctat actggttcgc ttattaaatg cccgtgtgaa aatttcccag
    30661 gattatcatc atcggcagtc tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg
    30721 cagggtggaa atgtggtgga aagtaactta tccgtgctga caccattggg acttgattcg
    30781 ttggtgaagc agacaaccat gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa
    30841 cttgatcacg ctggtgttgg acaaaattat gtggttatcc aacctacggc gcggcaaatc
    30901 ttcaaatgct gggacaacgc caagttttcc gctgtgattg atgccttaca tgctcgtggt
    30961 tatgaagtcg ttctgacgtc cggcccagat aaagacgatc tggcctgcgt caatgaaatt
    31021 gcgcaaggat gccagacgcc accagtaacg gcgctggctg gaaaggtgac cttcccggaa
    31081 cttggtgcgt taatcgatca tgcgcagctg tttattggcg ttgattccgc accggcgcat
    31141 attgccgctg cagttaatac gccgctgata tcgctgtttg gtgcgacaga ccatattttc
    31201 tggcgtcctt ggtcaaataa catgattcaa ttctgggcgg gagattaccg ggaaatgcca
    31261 acgcgcgatc agcgtgaccg aaatgagatg tatctttcgg ttattccggc ggcagatgtc
    31321 attgctgctg tcgataaatt actgccctcc tccacgacag gtacgtcgtt atgatcgttg
    31381 ctttttgttt atataaatat tttccctttg gcggtttgca gcgcgatttt atgcgtattg
    31441 ctcagacagt cgccgcccga ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg
    31501 aatgccctga tgtatttgaa ctgatcaaag tgccggttaa atcgcatacc aatcacgggc
    31561 gcaatgcgga gtattttgcc tgggtgcaaa aacatttacg cgaacatccc gtcgataaag
    31621 tcgttggatt caacaaaatg ccggggctgg acgtttatta tgccgctgat gtttgttatg
    31681 ccgagaaagt cgcgcaggaa aaaggctttt tctatcacct gacgtcacgt tatcgccatt
    31741 atgccgcctt tgagcgggca accttcgaac agggcaagcc gacacagctg ctgatgctga
    31801 cagataagca aatcgccgat ttccagaaac attatcagac tgaagcggag cgttttcata
    31861 ttctaccacc ggggatttat cctgatcgta aatatagcca gcagccagct aatagccgtg
    31921 aaatcttccg taagaagaat ggaataaccg aacaacaata tttattgttg caggtcggtt
    31981 cagacttcac gcgtaaaggt gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt
    32041 cgctgcgcca caacacattg ctatatgttg ttggacagga taaaccgcga aaatttgagg
    32101 cgctggcaga aaaacgcggc gtgcgcagta atgttcactt cttctcgggg cgcaacgatg
    32161 tctcggaatt aatggcggcg gcggatttat tactgcatcc tgcctaccag gaagcagcgg
    32221 gaattgtgct actggaagcg attactgcag gattaccggt actaacaaca gccgtttgtg
    32281 gctatgcgca ttatattgtc gacgccaatt gcggcgaggc tattgctgag ccattccgcc
    32341 aggaaacatt gaatgagatt ttacgcaaag cgttaacgca atcttcattg cgccaggcct
    32401 gggcggaaaa tgcgcgacat tatgctgata cacaagattt atacagtctg ccagagaaag
    32461 cggcggatat cataacgggt ggtctggatg gttgaactta aagagccgtt tgccacgtta
    32521 tggcgcggta aagatccttt tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa
    32581 ctggaaactc gccgcactct gcgatttgaa atggcgggca aaagctattt tctcaaatgg
    32641 catcgcggca cgaccctgaa agagataatc aaaaatttac tctcattgcg gatgccagta
    32701 ttaggcgcag accgcgaatg gaatgcgatt catcgactgc gggatgtcgg cgttgatact
    32761 atgtatgggg tggcatttgg cgaaaaaggc atgaatccgc tgaccagaac ttcatttatt
    32821 attaccgaag atctgacacc aaccataagt cttgaagatt actgtgctga ctgggcgact
    32881 aaccctccag atgttcgcgt aaagcgtatg cttattaagc gtgtcgcgac gatggtgcac
    32941 gatatgcatg ctgcgggcat taaccaccgt gactgttata tctgtcattt cctgctgcac
    33001 ttgccttttt ccggtaagga agaggagtta aaaatttcgg taattgacct gcaccgggcg
    33061 cagcttcgca cgcgcgttcc acgtcgttgg cgggataaag atcttattgg gctttatttt
    33121 tcttcgatga atatcggcct gactcagcgg gatatctggc ggtttatgaa tgtgtatttt
    33181 gccgccccgc ttaaagacat tctcaagcag gaacaaggac tgctgtcgca agcagaagca
    33241 aaagccacaa aaatcaggga aagaacgatt cgaaaatcgt tgtaacccgt aacttatttt
    33301 tgccaaaatt ttggatacag aataaatatg tctcaactca atgatagtga catcatcctt
    33361 tttgagtata attttcatta tcaaaatata agatctaaaa atactcttga tatagcattt
    33421 ggtattgaca gaaattttct ttttggatgt ggtgtagcca tcgcatctat tctattaaac
    33481 aatagagaaa tctcttgtga atttcatgtt ttcacagatt atatcagtga taaagacaaa
    33541 ttatattttt ctgatttagc aaaacaatat aattcaagaa ttaatattta tgttatcaat
    33601 tgtgataagc tgaagtcatt accaagcacg aaaaactgga cttacgcaac atattttcga
    33661 tt
//
LOCUS       DBFIFS010000030        33492 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.030, whole genome shotgun sequence.
ACCESSION   DBFIFS010000030 DBFIFS010000000
VERSION     DBFIFS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.030"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(176..316)
                     /gene="yjjY"
                     /locus_tag="N7A07_001316"
     CDS             complement(176..316)
                     /gene="yjjY"
                     /locus_tag="N7A07_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU2476009.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            412..1128
                     /gene="arcA"
                     /locus_tag="N7A07_001317"
     CDS             412..1128
                     /gene="arcA"
                     /locus_tag="N7A07_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU2476010.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1188..2540)
                     /gene="creD"
                     /locus_tag="N7A07_001318"
     CDS             complement(1188..2540)
                     /gene="creD"
                     /locus_tag="N7A07_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU2476011.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2598..4022)
                     /gene="creC"
                     /locus_tag="N7A07_001319"
     CDS             complement(2598..4022)
                     /gene="creC"
                     /locus_tag="N7A07_001319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU2476012.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4022..4711)
                     /gene="creB"
                     /locus_tag="N7A07_001320"
     CDS             complement(4022..4711)
                     /gene="creB"
                     /locus_tag="N7A07_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU2476013.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(4724..5197)
                     /gene="creA"
                     /locus_tag="N7A07_001321"
     CDS             complement(4724..5197)
                     /gene="creA"
                     /locus_tag="N7A07_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU2476014.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5408..6277
                     /gene="robA"
                     /locus_tag="N7A07_001322"
     CDS             5408..6277
                     /gene="robA"
                     /locus_tag="N7A07_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU2476015.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6274..6921)
                     /gene="gpmB"
                     /locus_tag="N7A07_001323"
     CDS             complement(6274..6921)
                     /gene="gpmB"
                     /locus_tag="N7A07_001323"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU2476016.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6973..7485
                     /gene="yjjX"
                     /locus_tag="N7A07_001324"
     CDS             6973..7485
                     /gene="yjjX"
                     /locus_tag="N7A07_001324"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU2476017.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7632..7958)
                     /gene="trpR"
                     /locus_tag="N7A07_001325"
     CDS             complement(7632..7958)
                     /gene="trpR"
                     /locus_tag="N7A07_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU2476018.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8048..9985)
                     /gene="sltY"
                     /locus_tag="N7A07_001326"
     CDS             complement(8048..9985)
                     /gene="sltY"
                     /locus_tag="N7A07_001326"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU2476019.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10196..11863
                     /gene="ettA"
                     /locus_tag="N7A07_001327"
     CDS             10196..11863
                     /gene="ettA"
                     /locus_tag="N7A07_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU2476020.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11970..12137)
                     /gene="nadS"
                     /locus_tag="N7A07_001328"
     CDS             complement(11970..12137)
                     /gene="nadS"
                     /locus_tag="N7A07_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU2476021.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12171..13403)
                     /gene="nadR"
                     /locus_tag="N7A07_001329"
     CDS             complement(12171..13403)
                     /gene="nadR"
                     /locus_tag="N7A07_001329"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU2476022.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13424..14806)
                     /gene="radA"
                     /locus_tag="N7A07_001330"
     CDS             complement(13424..14806)
                     /gene="radA"
                     /locus_tag="N7A07_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU2476023.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14855..15823)
                     /gene="serB"
                     /locus_tag="N7A07_001331"
     CDS             complement(14855..15823)
                     /gene="serB"
                     /locus_tag="N7A07_001331"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU2476024.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15929..16570
                     /gene="ytjB"
                     /locus_tag="N7A07_001332"
                     /pseudo
     CDS             15929..16570
                     /gene="ytjB"
                     /locus_tag="N7A07_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            16601..17617
                     /gene="lplA"
                     /locus_tag="N7A07_001333"
     CDS             16601..17617
                     /gene="lplA"
                     /locus_tag="N7A07_001333"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU2476025.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17649..17912
                     /locus_tag="N7A07_001334"
     CDS             17649..17912
                     /locus_tag="N7A07_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2476026.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(18073..18792)
                     /gene="deoD"
                     /locus_tag="N7A07_001335"
     CDS             complement(18073..18792)
                     /gene="deoD"
                     /locus_tag="N7A07_001335"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU2476027.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18849..20072)
                     /gene="deoB"
                     /locus_tag="N7A07_001336"
     CDS             complement(18849..20072)
                     /gene="deoB"
                     /locus_tag="N7A07_001336"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU2476028.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20124..21446)
                     /gene="deoA"
                     /locus_tag="N7A07_001337"
     CDS             complement(20124..21446)
                     /gene="deoA"
                     /locus_tag="N7A07_001337"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU2476029.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21573..22352)
                     /gene="deoC"
                     /locus_tag="N7A07_001338"
     CDS             complement(21573..22352)
                     /gene="deoC"
                     /locus_tag="N7A07_001338"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU2476030.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22610..24159
                     /gene="yjjI"
                     /locus_tag="N7A07_001339"
                     /pseudo
     CDS             22610..24159
                     /gene="yjjI"
                     /locus_tag="N7A07_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            24131..24994
                     /gene="yjjW"
                     /locus_tag="N7A07_001340"
     CDS             24131..24994
                     /gene="yjjW"
                     /locus_tag="N7A07_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU2476031.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(25208..25990)
                     /gene="yjjV"
                     /locus_tag="N7A07_001341"
     CDS             complement(25208..25990)
                     /gene="yjjV"
                     /locus_tag="N7A07_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2476032.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(25987..27060)
                     /gene="yjjU"
                     /locus_tag="N7A07_001342"
     CDS             complement(25987..27060)
                     /gene="yjjU"
                     /locus_tag="N7A07_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU2476033.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27182..27343)
                     /gene="ytjA"
                     /locus_tag="N7A07_001343"
     CDS             complement(27182..27343)
                     /gene="ytjA"
                     /locus_tag="N7A07_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU2476034.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27470..28075)
                     /gene="osmY"
                     /locus_tag="N7A07_001344"
     CDS             complement(27470..28075)
                     /gene="osmY"
                     /locus_tag="N7A07_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU2476035.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28468..30057)
                     /gene="prfC"
                     /locus_tag="N7A07_001345"
     CDS             complement(28468..30057)
                     /gene="prfC"
                     /locus_tag="N7A07_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU2476036.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30148..30825)
                     /gene="yjjG"
                     /locus_tag="N7A07_001346"
     CDS             complement(30148..30825)
                     /gene="yjjG"
                     /locus_tag="N7A07_001346"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU2476037.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30840..31286)
                     /gene="rimI"
                     /locus_tag="N7A07_001347"
     CDS             complement(30840..31286)
                     /gene="rimI"
                     /locus_tag="N7A07_001347"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU2476038.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31255..31668)
                     /gene="holD"
                     /locus_tag="N7A07_001348"
     CDS             complement(31255..31668)
                     /gene="holD"
                     /locus_tag="N7A07_001348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU2476039.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31771..32802
                     /gene="rsmC"
                     /locus_tag="N7A07_001349"
     CDS             31771..32802
                     /gene="rsmC"
                     /locus_tag="N7A07_001349"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU2476040.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32992..33078
                     /locus_tag="N7A07_001350"
     tRNA            32992..33078
                     /locus_tag="N7A07_001350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33026..33028,aa:Leu,seq:cag)
     gene            complement(33117..33353)
                     /gene="yjjZ"
                     /locus_tag="N7A07_001351"
     CDS             complement(33117..33353)
                     /gene="yjjZ"
                     /locus_tag="N7A07_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU2476041.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT         7889 a         9197 c         8537 g         7869 t
ORIGIN      
        1 ttggattcac cacgtttatt agttgtatga tgcaactagt tggattatta aaataatgtg
       61 acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct acaattgaac
      121 ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta gcctgttatg
      181 ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc tgagggaaag
      241 tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca agaacgcaat
      301 tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc aggcaggtca
      361 gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa catgcagacc
      421 ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa aagtattttc
      481 gaagcggaag gctatgatgt tttcgaagcg acagatggcg cggaaatgca tcagatcctc
      541 tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt
      601 ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct gactggccgt
      661 gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta catcaccaaa
      721 ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg taccatgaat
      781 ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa tggttgggaa
      841 ctggacatca acagccgttc tttgatcggc cctgatggcg agcagtacaa gctgccgcgc
      901 agtgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca gtcccgtgct
      961 gaactgctga agaaaatgac cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg
     1021 acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga aatcatcgcc
     1081 accattcacg gtgaaggtta tcgcttctgc ggtgatctgg aagattaatc ggctttacca
     1141 ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta tttccagata
     1201 cgtaattcat cgtccgttgt aacttcttta ctggctttca ttttcggcag tgaaaacgca
     1261 taccagtcga tattacgagt cacaaacatc atgccggcca gcgccaccac cagcacactg
     1321 gttcccaaca acagcgcgct atcggcagag ttgaggagtc cccacatcac accatccagc
     1381 aacaacagcg cgagggtaaa caacatgctg ttgcgccaac ctttcaatac cgcctgcaaa
     1441 taaataccgt tcattaacgc cccaatcaga ctggcgatta tccatgccac ggtaaaaccg
     1501 gtatgttcag aaagcgccag caagagcaaa taaaacatca ccaatgaaag ccccaccagc
     1561 aaatattgca ttgggtgtaa acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata
     1621 aaagtcagcg caatcagcag aatggcatac ttagtcgccc ggtcagttaa ttggtattga
     1681 tcggcgggcg tcgtcactgc gacgctaaac gcggggaagt tttcccagcc ggtatcattg
     1741 cctgaagcaa aacgctcacc gagattatta gcaaaccagc tgctttgcca ttgcgcctga
     1801 aaacctgact cactaacttc ccgtttggct ggtagaaaat cacctaaaaa actgggatgc
     1861 ggccagttgc tggttaaggt catttcgcta ttacgccccg caggcaccac agaaagatcg
     1921 ccggtaccgc ttaaattcag tgccatattc agcttcaggt tctgcttccg ccagtcccct
     1981 tcgggtaaag ggatatgcac gccctgcccg ccttgctcta acccgatgcc gggttcaata
     2041 gtcagcgccg ttccgttaac ttcaggcgct ttcaccacac caataccacg cgcatccccg
     2101 acgctaatca caataaatgg cttgccaaag gtgatatttg gcgcgtcgag ttcgctaaga
     2161 cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt gccagacctg accggtataa
     2221 atccctatct tgcgttcttc cacgttctga ttgccatcaa ccatcaatga ctcaggtaac
     2281 caaaaatgga taaaacttcg tttccgctcc acggttttat cctcttcctg caccgtataa
     2341 agctcggtca caggaatagc gataagaggc ccaacgagtt tttgcggccc gctggtactt
     2401 tggcgaatcg catcttccac atcgctacgg taatcggcac gttcgacaat cacctgccga
     2461 atcagcatta tcggaatcaa caacagcaat actgcaccaa acagggtggt cattttccag
     2521 aacagggggg atttcaacat agtcttctcc tttgcaatgg cagcaggata cgaaggctat
     2581 gtgggaagaa tttgaagcta tgtgaagtga cggtgaagcc gaagcgaggc cagcacgcca
     2641 ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca aacgggcgac ctcactgaca
     2701 aacgccaacc ccagaccgct gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt
     2761 tcaaaaatac gtgacagcgc gtagtcagga ataccactac cggtatccag caccttaagg
     2821 gcgacgtgtt cctgatccac ttcggcgctt agcgttatgc gaccgctctt gggggtaaaa
     2881 tcgatggcgt tatccagtaa attccccagc gcctgctcca gtaacgccgg ttcagcagca
     2941 acgttaacct cagtaggcat aacatgcaaa gtgatgtttt tttctgccaa ctgcacggtg
     3001 cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa cagcagtcag aacgacttcc
     3061 tgacgattct ccagtcttgc ctggcgtagt aacgtttcta ccaatgcctg catacgcgca
     3121 ttttgcgtca gaatgttgtc ggtaaaacga gccaccactt ccggtggcgg accttcgcgt
     3181 aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt ttagctcatg ggttaacgca
     3241 taaacatact gctcaatata gtttttccct tccagcttca cgcgcatact ttccagcgcc
     3301 tgcgcaagct tacgcaactc gctactgccg agttcaggaa gaggaacggg cttattgtca
     3361 gtgacggaat cggcatagcg agtgagccgg gcaatggagc ggttgatcca ccaaaccatg
     3421 cctgcgccaa tcaccagtgc aatccccagc aaaatggcgc tggcccataa aattcgccgc
     3481 tcgctgcgct taatgaccgg agccatcgcc gcgttcggtt tgcctacgct caaaacgcca
     3541 ataagccgcg agccgtccat aatcggcgcg gcaacataca tcaccgaact ttcgggatcg
     3601 gcaggatttt gcaacgtgct gcgcgcacca tactgaccac gcaacgttag ccagacgtca
     3661 ttccagcgcg aataatcctg cccaacggct ttatttgccg aatcgaacaa tactttgccc
     3721 tgcgcatcgg tcatatagac atgatattca ttgcgcactt tgttaatgcc accgatatta
     3781 gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg ccagttgccc atgcgttggg
     3841 tccccagaga gcaaatcggg acgcgccagc tccgccagca acgttgcggt gtcgatcagc
     3901 gtcccctccg ttgctcttcg cacgcccggt ttaacttctt tgacaaaaat agccagtacg
     3961 aaccaggccg ccaccgccac cagtaaaaaa tagcccagca acaaccgcat gccgatacgc
     4021 attacaggct cctcaggctg tatcccatgc cgcgatgagt attaatcggt gaaagatcgg
     4081 ggttgatggc gcgcagcttg gcacgcagcg ttttaatgtg ggtatcgacg gtgcgatcgt
     4141 aggtgtcctg cgcatcttcc cacacgatat ccatcagttg ctggcgggac cagacgcggc
     4201 ctggcgactt gagtaacgtc ttcagcaata aaaactcata ccgcgtcagc gttaatggcg
     4261 tgtcaaacca gctgatctgc gccgcgggtt cattcaattc aaaatgacca atacggatga
     4321 cgggagacgg cgtcgagaac ttcttcaccc gacgcagcaa ggtgcgcacc ctagcgcaca
     4381 cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc gccaatttcc agcccaagca
     4441 ggcgatcgac ctcttcactt cgggccgtca ggaacagtac aggtaacgcc ggatggagcg
     4501 ccagtaactg gcggcacaat tcaaagccac tgatatcagg caaaccaacg tcgagaatca
     4561 tgacatcggg aacctgctgg cgagctttat ccagcaccgg caagcctcgc tcaaagacct
     4621 cgacggcaaa accttcctgc tgcaacatgt agaccagcgt gtcggctatc ccttgctcat
     4681 cttccactaa ccagaccgtt tcccgttgca taaatcgcct ctgttattgt cgccacggca
     4741 tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc tacaactttg tcggagtaag
     4801 ccagataagc gagcgcgttg cgtttggcgt cataaaagcg cacgacctgt aacgacttaa
     4861 agaccaggga cgtgcgtttt ttgaatacta cctcgccctg agctttgccg tttttaatac
     4921 gatccgacag ttcaatcggc ccgacttgct gacaagaaat agccgcatcg gaggtatctt
     4981 ccgccagacc caatccccct ttaataccac ctgttttcgc ccggctcaca taacaggtga
     5041 catttttcac atcgggatcg tcaaaggctt ccacaacaat tttgtgatcc gggccgatca
     5101 ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg agccaatggc cccagcatta
     5161 tcaggcttaa agacaggatc aaatgcttgt atttcatatt gttaccattt ttatctatta
     5221 cacgagcaat tagttcgtca cggtaaataa catattgatt aagaacaaaa tctcaatact
     5281 tttatttccg tcaagaccta aaacatactc tactaagaaa aaaacactga atgctaaaac
     5341 agcaaaaaat gctattatcc aattacctga tgtcaggtgc tcgttgttga aaggatgagg
     5401 atattttatg gatcaggccg gcattattcg cgacctttta atctggctgg aaggtcatct
     5461 ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca ggttattcca agtggcactt
     5521 acagagaatg tttaaagatg tcactggcca tgctattggc gcgtatattc gtgctcgtcg
     5581 tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt ccgattctgg acatcgcgct
     5641 gcaataccgc ttcgactctc aacagacatt tacccgcgca ttcaagaagc agtttgccca
     5701 gactcctgca ctttaccgcc gttctcctga atggagcgcc tttggtattc gcccgccgct
     5761 gcgtctgggt gaattcacta tgccagagca taaatttgtc accctggaag atacgccgct
     5821 gattggtgtt acccagagct actcctgttc gctggagcaa atctctgatt tccgccatga
     5881 aatgcgttat cagttctggc acgattttct cggcaacgcg ccgaccattc cgccagtgct
     5941 ctacggcctg aatgaaacgc gtccgagtca ggataaagac gacgaacaag aggtattcta
     6001 taccaccgcg ttagcccagg atcaggcaga tggctatgta ctgacggggc atccggtgat
     6061 gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga
     6121 gtttatcctg acggtatacg gaacgtgcat gccaatgctc aacctgacgc gccgtaaagg
     6181 tcaggatatt gagcgatact acccggcaga agacgccaaa gcgggagatc gcccaattaa
     6241 tctgcgctgt gaactgctga ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg
     6301 ggcgtccaga tgcgagatgt cccctgcggt ttcaacgacc cagccagacg ccagccacag
     6361 gctttcctga taatccacgc gcgaaataga acagttacgc agacgtaagc ggcgctctgc
     6421 ccatgctggt aatccgagaa tcgtactcac cagacatccc agtgcaatac catggctcac
     6481 cagcagcggt cggcttccct gcggtaagtc ccggcaagat tccagtgccg cattgacgcg
     6541 atcgctgagc tcctgcattg actcgccttc aggaatacgc ccatcaacgg tgccattgac
     6601 cagctgccga cgccagttct cttcttcttc ggtcagagaa tcgatatgtc ttttttccag
     6661 cacacccatg tttaattcac gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg
     6721 ggcgatgatt tccgccgtac gccgggtgcg tcctaaatcg ctactgatga tatgcgtaat
     6781 gccaagctct ttggcacgcg tcgccacctg catcgcctgt tgctcacctt tggcggtcag
     6841 cgggctgtca gactggccct gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg
     6901 gactaggtat acctgtaaca tgcttttttt ccgtatactg ctgtaacgtt aattttctga
     6961 gtaatgctga ttatgcacca agttgtctgt gcgaccacca atcccgctaa aattcaggcc
     7021 attctgcagg catttcacga gatcttcggc gaaggatcct gccatattgc atccattgcc
     7081 gtcgagagcg gtgtaccgga acagcccttt ggcagtgagg aaacgcgcgc tggcgcacga
     7141 aaccgggtag ccaatgcccg ccgtttactt ccagaggctg atttttgggt ggcgattgaa
     7201 gctggcattg atggcgacag cactttcagt tgggtggtga ttgaaaacac cacccagcgc
     7261 ggcgaggcgc gttcggcaac tttgccgttg cctgcggtga tattggaaaa agtacgtgaa
     7321 ggtgaggcgc ttggtcccgt gatgtcgcgt tataccggta ttgatgagat tggtcgtaag
     7381 gaaggggcga tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag
     7441 gcggtgatcc tggcgctgag tccgtttcat aatgcggtgt attaagcgcc tgatgcgacg
     7501 ctgccgcgtc ttatcatgcc taccaaacat attgaaatta cgggtatttg taggacggat
     7561 aaggcgttca cgccgcatcc ggcattcggt gcacgatgcc tgatgcgcca cgtcttatca
     7621 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactggcg cagctccacg
     7681 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcctgc gccgagttca
     7741 ttttttaact cacgctggct catttcgccg cgcaacagct cttcgacaat acgcacgcga
     7801 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
     7861 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
     7921 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
     7981 gggaagcaaa atgcctcccc cggttgtact cgctaaagag tacgatagca tatcataaac
     8041 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
     8101 tgaaatagcg gtagtaagcg tcataagcca gcacgttctt cacataaccg cgcgtctcgg
     8161 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
     8221 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattac
     8281 caaattgctg ataaacatat tgcaggtaac tggtgccaat gttgatattc gtttccggat
     8341 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
     8401 ccgtgcccgg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
     8461 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tccttcccac
     8521 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
     8581 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggttg ttgaaagcat
     8641 accgcgccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
     8701 tacgcgcggt gttatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
     8761 cctgagtcag ggcgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
     8821 cgccgatgcg ttgtgcagca accatcgggt agaaaccacg ctgttgcatg agttgatgca
     8881 aaatctcttt tgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
     8941 gccattcatc tttctctttc gcttccatag gcagacgcgc cagccaggta ttcaggccgc
     9001 gacgatcgcc agtgccaagc gccattcgca cacggcgttc aataagcgaa gtagattgcg
     9061 agcgcataat ggcgtcatcg cgccatttcg cctgctcgtc ggtgacatcg ttgcccatca
     9121 aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc tgctgcgcct
     9181 gggcaagcga tgggatcatc agccgtgcat tttcagcatc ctgccgcgcc acactggcaa
     9241 acgccaccgc cgccatttga cgggtaaaat cggtcgctcc ggttgtacgc gcgaaggtca
     9301 gtaccgtatt ggggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
     9361 caggcatctg ccctgccagc actgttacca ggccagtgtt acccgctttc atcgccagac
     9421 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
     9481 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
     9541 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgtgcttcgg
     9601 tagttccggg cttttccggg ctaaaggcta acaggccacg ccagtcttca cgccgcgcca
     9661 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
     9721 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
     9781 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
     9841 tttgtcgatt atcccaggcc tgcttgattt gcgcgtaacg actacgctgc tcatccagtg
     9901 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
     9961 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    10021 cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    10081 cactttttta cttttactgc gagggtgatc gaccggctgg gattaggcgg ggaaaaaggc
    10141 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    10201 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    10261 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    10321 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    10381 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    10441 ggaacacacc gtacgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    10501 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    10561 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata atctgaacgt
    10621 acagctggag cgtgcggcgg atgcgctacg tctgccggac tgggacgcga aaatcgctaa
    10681 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    10741 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cctggctgga
    10801 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt acccacgacc gttacttcct
    10861 cgataacgtt gcgggctgga tcctcgaact tgaccgcggt gaaggtattc cgtgggaagg
    10921 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    10981 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    11041 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gaactgaaca gcaccgaata
    11101 tcagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    11161 agtgctggaa gtcagcaacc tgcgtaaatc ctatggcgat cgtctgctga ttgatgacct
    11221 gagcttctcg atcccgaaag gagcgatcgt cgggatcatc ggtccgaacg gtgcgggtaa
    11281 atcgaccctg ttccgtatga tctctggtca ggaacagccg gacagcggca ccatcacttt
    11341 gggtgaaacg gtgaaactgg cgtcggttga tcagttccgt gactcaatgg ataacagcaa
    11401 aaccgtttgg gaagaagttt ccggcgggct ggatatcatg aagatcggca acaccgagat
    11461 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgcgt
    11521 tggtgaactc tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    11581 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    11641 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    11701 gttcctcgac cgtatcgcca cgcacattct ggattaccag gatgaaggta aagttgagtt
    11761 cttcgaaggt aactttaccg agtacgaaga gtacaagaaa cgcacgctgg gcgcagacgc
    11821 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    11881 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    11941 agacctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    12001 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggccacc
    12061 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    12121 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcatcacgg ttatctctgc
    12181 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcttcc
    12241 tcttcaaccc gcacaaattc gatattgttc tcttccagca tctccaccag cagattctgg
    12301 aactctttac gatccaccga actgccgagg ctgcgtaaac catccaccac ccacggcgtg
    12361 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    12421 ggatgttccc gcccttcgta ctttttgcag aacgcctgag tggtgacaaa atcggtatcg
    12481 ataaatgcca ctttattggc atatttcacc gcaaaatcaa tgtattgagc gtggcccagc
    12541 gcgattttat cgtagtcaga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    12601 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    12661 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    12721 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    12781 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    12841 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcagatccgg ctggatcccc
    12901 ttttcagcca taaacttttt gataccgttg ctccacacat cccagccgtg cggatacggc
    12961 tccatgccct cttcgttgaa agcatgaatg cgaatatttt tctgatattt aaaagtttgc
    13021 aataaccagc gcagacgatc cggcacggtc ggctgctgcg acatggcact gtcttcgaac
    13081 aacgcgcggt cacgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    13141 caggcgcgct ggataaggta gatatgtccg gtatgcagtg ggtagaactt accgaatacg
    13201 acaccgatcg ttttcttctg ccggggaaac tcaagcccca aaaaacggtg caacgcctcc
    13261 agcttttgcg cgctggggct tttgattttg gcattcagta actggcttaa ataccctttg
    13321 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    13381 ttcaggtaat caaatgacga catatctccc tccgtatttc tcattataag tcgtcgaaca
    13441 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    13501 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaga
    13561 ttcgttcctg accgctgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    13621 ccaccagatc ctgcggtagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    13681 ctaagtcggc gctggtttcg gttaccttta cgccgccgac cacgttcaca aacacatcct
    13741 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    13801 tttgttccag ccccactgcc acgcggcgcg gattcgccat catcgagtga tcgaccagcg
    13861 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    13921 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    13981 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    14041 tgcgcaaggt gcgaaaacgg gagtcggcat cgccatctaa aagcaccgaa cagtcgatac
    14101 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgc cccaccatga
    14161 caatcgccac accgcgcgtt ttggcgaagc gtgtcaaata agccgccgtt tcacgcacct
    14221 gcgccacgct accaggcgat gactgtacat ccgccatatg catcacctgg atcgagtcaa
    14281 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgttcg atgctggttt
    14341 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    14401 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    14461 gcgtttgcag tagcagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    14521 ttcctggcac cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    14581 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    14641 cggcatagcc gctgagacgc tcgttacgcg ccaccgtagg cgacgcagca agacgcacct
    14701 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    14761 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    14821 atgctgtgcc gcccgcagga tggcgggcga gcaattactt ctgattcagg ctgcctgaga
    14881 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    14941 cttttggctt ggcatggtag gcaatcccca gccctgccgc tttgatcatc ggcaggtcat
    15001 tggctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    15061 gagtcagagt tttcgctttg tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa
    15121 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    15181 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    15241 tttccagctt gagcaccagt tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    15301 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    15361 taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc cgccaccatc tcgcccgttc
    15421 cggccagttt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    15481 tcaccagcaa acccggcgtg cgcaggtgcg ggatttttcc cagcggcgcg acatccagct
    15541 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    15601 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    15661 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgacctg
    15721 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    15781 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    15841 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    15901 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctgca
    15961 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggggcgtc
    16021 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    16081 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    16141 aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg
    16201 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    16261 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    16321 aattgccggg aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    16381 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    16441 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg
    16501 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    16561 gaaaaaagag tgccccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    16621 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    16681 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    16741 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    16801 cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    16861 tacctttatg gctggcaagc cggagtacga taaaaccatc tccacgtcga ttgtgctcaa
    16921 tgcactgaat gcgctcggcg tcagtgccga agcgtccgga cgtaacgatc tggtggtgaa
    16981 aaccgccgaa ggcgaccgca aagtctcagg atcggcctat cgcgaaacca aagatcgtgg
    17041 cttccaccac ggcaccttgc tgctcaatgc cgaccttagc cgcctggcaa actatctcaa
    17101 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    17161 cctcaccgag ctgctgccgg ggatccccca tgagcaggtt tgcgaggcca taaccgaggc
    17221 ctttttcgcc cattatggcg agcgcgtgga agcggaaatc atctccccgg acaaaacgcc
    17281 agacttgcca aacttcgccg aaacctttgc ccgccagagt agctgggagt ggaacttcgg
    17341 tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact
    17401 gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtgtttaccg acagcctcaa
    17461 ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat
    17521 gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg
    17581 ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg
    17641 aaggaaatat gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc
    17701 agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa
    17761 gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta
    17821 gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat
    17881 gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgg
    17941 attttatttt ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg
    18001 aatagatact aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc
    18061 agcgaaacac aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat
    18121 gtcgttgaag gtggtctgac gctcagcggc agtggtttgc tcgtgagtgc ggatgtggtc
    18181 agacacggtg cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc
    18241 agccgcttcc atttccacgc cgagaatgcc gtatttttcc atcacgtcga acatttcgcc
    18301 gtccggagag tagaacaggt cagcggagaa caggttacca acgcgagcat caatgcccag
    18361 tgctttagct gcatctactg cgttacgcac catgtcgaag tcagcgatag cggcaaagtc
    18421 atggtcttta aaacggatgc ggttaacttt ggaatcggtg caggcaccca taccgataac
    18481 gacgtcgcgc agttttacgt gcggcagaac tgcgccacag gaacccacgc ggataatttt
    18541 cttcacgccg aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat
    18601 accgtgaccc attacggaaa ttttgcggcc tttgtaagta ccggtgaagc ccagcatacc
    18661 gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg
    18721 cagcgggtcg cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg
    18781 tggggtagcc attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc
    18841 caaatccatc agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc
    18901 agagtctggc cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc
    18961 gggccgtaaa ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga
    19021 tcacagccgt ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc
    19081 tccggcagac ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg
    19141 tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct
    19201 tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca ggccagtcgc tttcactttt
    19261 ttggtgatac cgcagttggc gtagatgtct gcaattttac cgaccgaaac cacctggccg
    19321 tgtttttcat caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg
    19381 tgacggttac cggtacgctg gaagttaccg gctttgtcgc cgataaacgg acgagcgata
    19441 acacgaccga tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg
    19501 tagagtttat ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg
    19561 gaggtataga aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg
    19621 accgtaccgg aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt
    19681 ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac
    19741 agaaccggga caccggcaat ttcccagtga ccagacgggg tatctttacc ggatgacatt
    19801 tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg
    19861 aaaccggtag aaccttcgtg tgctttcgcc agccccagac gggtcagatt tggcagattg
    19921 agcgggcctt tacgaccgtt atcagcttcg cctttggcac aagcttctgc gatatgaccc
    19981 agggtgtcag ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat
    20041 gagtccagca ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt
    20101 taaatgatca gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt
    20161 tccggtgctt tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag
    20221 ctgttttcgt ctttcgcgtg gataaccgcc agcggacgct ggccgtctac ctggtcgccc
    20281 agacgtgcca tatcagtaaa gccgacgctg taatcgatgg tgtcagatgc ctgacggcgt
    20341 ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa
    20401 ccttcggtat cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag
    20461 ttctcaacga agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc
    20521 gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc
    20581 agtttgccgg agataagcat ctccacacac agcgccatcg tgacatcaaa cagacgcggg
    20641 ttacgatatt cacccgtcag gaactgcacc gcttcacgta cttcaaccgc gttaccggca
    20701 ctggaggcca gcacctgatt catgtcggtg agcagtgcgg tggtgcgcac gcctgcgccg
    20761 ttagccacgc caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac
    20821 gcgccgctac ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc
    20881 gccaggatcg aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc
    20941 gcgtagaaac gtttatccgc cggagccagt gagctggtct gaccgataat cgccacgccg
    21001 acgtctttaa taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg
    21061 gattccagtt tgtcgagcgt accgccagta tgaccgaggc cgcgaccaga gatcatcgga
    21121 atatagccgc cgcaggctgc gaccatcggc cccaacatca gcgaagtcac atcgccgacg
    21181 ccaccggtgg agtgtttatc aacaatcggg ccattcagat gcaggctttt ccagtcgaga
    21241 acggttcctg aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg
    21301 tggaagaaaa tggtcatcgc gagggcggca atctgccctt cggagatagt gttgtcgcga
    21361 ataccgttga taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta
    21421 cgaataattt cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggtggct
    21481 tttgccggat gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat
    21541 aagcgcagcg catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg
    21601 ccgtgaccca gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga
    21661 cgcgcatcag cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca
    21721 tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata
    21781 tcacggatca cttccatcat gatgcgcgcg ctttccggcg tcgcgttcac agccacttta
    21841 ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg
    21901 atcagcgctt cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct
    21961 gccgcgcaag cctctttaca ggctttcacc aggtcaaaac caacctgctc gttacccgcc
    22021 atcagcgcgc ggtacgggaa cacaacgtca acttcatcag caccgtaggc gattgccgca
    22081 cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta
    22141 gcaatacgga tttccggggt gccctgctct ttcagtgttt tgcgagcaat cgggataaag
    22201 cgaggataga tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga
    22261 gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt
    22321 gcacgcaggc tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa
    22381 attcaccttg cgagtttgtt agtattctaa catctactcc gcaacacact tcgatataca
    22441 tcacaattaa ggaaatctac ttacaagttt gcatcactat aatgcgatct ggttcaaata
    22501 attcactttc aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca
    22561 cgaaacttct tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc
    22621 tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc
    22681 ggagcagaag cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct
    22741 tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca
    22801 tgcgccgtac aaaccgcgct atgtcttacc cgattacgcc cgttttctgg cgaacggttc
    22861 cgaatggctg gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat
    22921 tctttaccac cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc
    22981 gttgttgcaa ccgtatgtta taattctaac acaagacgag atcgatgttc gaataaaacg
    23041 tttctggcgt tacctcgaca gaaccctgcc agacgccttt atgcacgcca atatcggccc
    23101 gtctgattcg cccattaccc gcgcgatctt acgtgcagac gcagatttga agcaggtttc
    23161 accgaacctg acctttatct acgatcctga aatcacccct gatgacctgc tgctggaagt
    23221 ggcgaagaac atctgtgaat gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa
    23281 aattttcaca aaagggggct acgggattgt gagctgttac aactcactgc cgctggcggg
    23341 tggtggcagc acgctggtac gccttaacct gaaagccatt gccgagcgca gcgaatcgct
    23401 ggatgacttc tttacgcgca ctctaccgca ctactgccag cagcagatcg ccatcatcga
    23461 tgcgcggtgt gaattcctct atcaacaatc acacttcttt gagaatagct tcctggtgaa
    23521 agaagggctg attaaccctg aacgttttgt gccaatgttt ggcatgtatg ggctggcgga
    23581 agcggttaac ttgctgtgtg aaaaagaagg gattgccgcg cgctacggta aagaagccgc
    23641 cgcaaatgaa gtaggttatc gcatcagtgc acagctggcg gagtttgtcg ccaatacccc
    23701 cgtgaaatat ggctggcaaa aacgcgccat gttacacgca cagtcgggga tcagttccga
    23761 tatcggcacc acgccgggcg cgcgtttgcc gtatggcgat gagccagatc cgatcaccca
    23821 tctgcaaact gtcgcccgca tcatgcttat tattattctg gcatcagcga cattctgacg
    23881 ctcgacgaaa ccatcaaacg taacccgcag gcgttggtac agctttgcct cggtgccttt
    23941 aaagccggaa tgcgtgaatt taccgccaat gtcagcggta acgatctggt tcgcgttacc
    24001 ggttatatgg tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac
    24061 accacctggc tgggcgaaga agccgcacgc aacactcgta ttctggaacg ccagccgcgc
    24121 gtgataagcc atgaacagca gatgcgcttt agtcagtaag attatcccct tctcctgcgt
    24181 tgacgggcca ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa
    24241 ctgtcacaat ccgtggacga tgggacgttg caatgactgt ggggagtgcg tgccacagta
    24301 tccgcatcag gcgttgcaga ttgttgacgg caaagtggtg tggaacgctg tggtctgcga
    24361 gcaatgtgat acctgcctga agaggtgtcc gcaacatgcc acgccaatgg cgcaatccat
    24421 gagcgtggat gaggtgctta gccatgtccg caaagcagtg ctgtttatcg aagggataac
    24481 ggtgagtggc ggcgaagcca cgacccaact gccgtttgta gtggcgctgt ttactgctat
    24541 caaaaacgat ccgcaactgc gccacctcac ctgcctggtg gacagtaacg gtatgttgag
    24601 cgaaaccggc tgggaaaaat tgctcccggt gtgcgacggc gcaatgctcg atctcaaagc
    24661 gtgggggagc gaatgtcatc agcaattaac cggacgcgat aatcagcaga ttaagcgcag
    24721 catctgtttg ctggcagagc gcggtaagct ggcggaactg cgtttgctgg taatccctgg
    24781 ccaagtggat tatttgcaac acatcgaaga actggcggcg tttatcaagg gagtcggcga
    24841 tgttccggta cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg
    24901 ggcgagcgcc acgccggaag acgttgagcc gttggctgat gcgttacggg agcgcggggt
    24961 gagccggtta atatttccgg cgctgtattt gtgacatgcc ggatacgacc tacggttcgg
    25021 cacagacttg taggcctgat aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact
    25081 gccggatgcg gcgtgaacgc cttatccggc ctacggttcg gcacaaacct gtaggcatga
    25141 taagacgcgt taagcgtcgc atcaggcatt atgcgccgac tgccggatgc tgcctacgaa
    25201 gggcaaacta cccacgaacg ttaaacaccg catacgtgtt attaagcaac acttcggcaa
    25261 tctcatcttc tggttccggg cgcaactcgc aaagcacgtc aaacacccgc gcagcctgtt
    25321 ccgggcggtt aggctgcccc tgaaaaccgt tgagcggcat atccggcgcg tcggtttcca
    25381 gcaacaacga tgccagcggt aattttgcga tgacaacgcg ggttttactg gcgcgtggat
    25441 aggtgatagt accgcctacg ccgattttgt agcccagctg tacaaaccgc tcggcctgtt
    25501 gcaggctgcc ggaaaaaccg tgaaccacgc cagtgcgcga taaatcgtgg cgtttaagat
    25561 gcatcgccag tttgtcgtgt gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt
    25621 tcgccagttt cagttgttcg tcgagaaacc actgctgcct ctcaaattgc ggatcgtcgc
    25681 cgaagagatc cagaccgctc tcccccaccg ccaccacctt cgccggacgc ctttccagcg
    25741 cctgctgtag ctgatcaaga gacacatcgc tatgtttttc caacatacca ggatgcaagc
    25801 ccaatgcggc atacagcggt tgataatttt ccgctaacgc ctgcacgcgg gcaaaatttt
    25861 ccgcctcggt tgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc
    25921 tggcctcttc atcgccaata aaaggcggga aatcaaaatg gcagtgggtg tcgataaaac
    25981 ggcaaatcaa gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct
    26041 cacttcggca accagcgtat cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg
    26101 cgtcaccact ggcaccagat ggcgggtaag cggcgctttt tcagttaata gcttgccaac
    26161 cgtggcgagg aaataacgac cgcataaacg cccaagttta tagtcttcac gcagcgccgg
    26221 aatccgactg ccaagcgcga tactatgtaa tggcttcggc ggataaattt cgaatatccg
    26281 cagcttgccc ggtggtttct caataaattg ctgaatgtca cgatagctgg tttcatgatg
    26341 ctgcaccaga ttgaccagcg gctgcaggct actgtcaccc agccagcgtt ccatgcgttt
    26401 gaaccactgc ggcgtgtagt acatttgtga cggcacagtg cggatgacga ccaacgtttt
    26461 agcgccctgc cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc catccaggta
    26521 gttaatgcct tccagcgaca ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg
    26581 aatcacatcc agccagtttt gtttggttgg taaaaagtaa ttcggcgcgt agtcatcctg
    26641 acgacaggcg cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat
    26701 ttgcaacggc atctggcttg cggtggcctc caccagccag tcgagatcga taagatttcc
    26761 tccacggaca aagcgcaatg gatcgaaaaa ttcgcgtttt gtggtatagc gcatgatgac
    26821 tttgcgcgcg taaccgggct gattgcagat atacgccgag aggttctgcg ccccggcaga
    26881 tgtgccgaga taaagatcga aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc
    26941 agccgtgaaa attccacgct gtccgccgcc ttcgcacacc agagctattc gtccaggctg
    27001 gaacggcctt agcgacaacg gcgcaatatt accaagcgtt acaggtattc gctgccccac
    27061 ctctgccttc ctgtttttgt tgtgaaagaa tgacaaagta acgcaattta ttattcgcta
    27121 aaaccgcaaa agccagtccg ctggactggc tttggataaa attttcttaa tggtctgaaa
    27181 tctagggtcg ttttcggccc atgaacaaac tcaccaggaa cagaataatc ccgacgacaa
    27241 agacaatttt agctgcgcct gcagcggtac cggccagacc accaaaccca agtgcggcgg
    27301 cgattaacgc gataaccaga aatatgatgc cccaacgaaa cataagcgtc tcctttacca
    27361 tagttaatgt caccgcttga tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg
    27421 gtgcacatcg cgcctcccga caaagttcgg gaggacgaat tacgacgaat tacttagttt
    27481 tcagatcatt tttaacgctt ttcacaccat ctaccgcttt ggcgatactt tcagcacggt
    27541 cactttgtgc ctgagaatct acgataccag agagctgaac cacgccatcg gtggtttcaa
    27601 ctttcacatg acgggaaggg acgatatcgt ccgccagcaa tttggctttg atttcactgg
    27661 tggtggcggt gtcacccgcg tagcccttca cagagccttc tttagcgtcg cgaacgtgca
    27721 gtttgtcgct gacagaggtc accccttcaa cacctttcgc cactttcact gcctcttcgg
    27781 cctgggcctg gctttcaacg aaaccgctca gggtcacgac tttttgatcg gttttgacag
    27841 agatatcggt gctcttgatg ttgtcatgat ccaccagcgc cgccttcact ttcgcggtga
    27901 tggcgctgtc atccatgaaa ttaccgactt tattcataga gctatcgact ttttgccctg
    27961 cgctttcatt ggtagtctgc gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag
    28021 aggtcaacat tacagccagc agagttttcg aaatcttcag tcttgtcata gtcatcgatt
    28081 tattcctgta tgtttgctcg taatttgagc ctggcaacac gaggttgcat tgctgaatag
    28141 ggagagactt caccctctac agaagtcaat ggtcgccatc acaaaagcga tgagtgatga
    28201 ataacgacca ttacagcctc tgaatcagtt attaatatcg gtagaatgac aatcgacggc
    28261 tttagatact gatatctacg cattgaacgg tatttaacgc cgtcagaaat gtcatcactt
    28321 tgttaaatat agatcacaat tttgaaaccg ctcgggatat cagcgaaaac ataagcaaaa
    28381 gtgaatgttt taagaacatt ccgtaagcgg ctaataagga agggaaattg acaggacgca
    28441 gcggctaccg cgccctggag gcaagaatta atgctcgcgg gtctggtgga actgaacgtc
    28501 cggataacgt tcctgtgcca ggcgcaggtt gaccatgctg gtagcgatgt aagcgaggtt
    28561 atcgccgcca tcaagcgcca gttggctttc gttcttacgc ttgaactctt cgaatttctt
    28621 cgcgtctgca cattctaccc agcgggcagt ggcaacgttg actgactcat acactgcttc
    28681 aacgttgtat tcgctcttca ggcgcgctac caccacatca aactgcagca caccaaccgc
    28741 accaacgatc agatcgttgt tggagattgg acggaacacc tgcaccgcgc cctcttcgga
    28801 aagctgtacc agccctttga gcagctgttt ttgcttcagc ggatctttca ggcggatacg
    28861 acggaacagt tctggtgcga agttcggaat accggtgaac ttcatcatct caccctgggt
    28921 aaaggtgtcg ccgatctgaa tggtgccgtg gttgtgcagg ccgaggatat cgcccggata
    28981 cgcttcttca acgtgcgaac ggtcacccgc cataaaggtc agcgcgtcgg agatcaccac
    29041 atctttcgca gtgcgcacct ggcgcagctt catgcctttt tcatatttac cggacaccac
    29101 acgcataaac gccacgcggt cgcggtgttt cgggtccatg ttggcctgaa ttttaaatac
    29161 gaagccggta aatttatctt cgctcgcttc tacggtacgg gtatcggtct gacgcggcat
    29221 cggcgcaggc gcccactcca ccaggccatc cagcatatga tcaacgccga agttacccag
    29281 cgcagtaccg aagaataccg gagtgatttc gcccgcaagg aacagctctt tgtcgaactc
    29341 gttagacgcg cctttcacca gttccagttc gtcacgcagc tgctgtgcca gatcttcacc
    29401 aaccgcagca tcgagatccg ggttattcag ccctttaaca atgcggactt cctgaatggt
    29461 gtgcccttta ccgctctgat agagataggt ttcatcttta taaaggtggt aaacgccttt
    29521 aaacagcttg ccgcagccaa ttggccaggt gatcggtgcg cagccaattt tcagctcgtt
    29581 ctcaacttca tcgagcaatt ccatcgggtc gcggatatca cggtcaagtt tgttcataaa
    29641 ggtgaggatc ggcgtgtcgc gcagacgggt aacttccatc agcttacggg tacgatcttc
    29701 aacacctttt gcggcgtcga taaccatcag gcagcagtcc accgccgtca gggtacgata
    29761 ggtatcttcc gagaagtctt cgtgccccgg ggtgtcgagc aggttaacca ggcaatcgtg
    29821 atacggaaac tgcatcacag acgtagtaat ggagatccca cgctgctttt ccatctccat
    29881 ccagtccgac ttagcgtgct ggttggaacc acggcctttt actgtaccgg cggtctgaat
    29941 ggcctgtccg aacagcagca ccttctcggt gatggtagtc ttaccggcgt ccgggtgaga
    30001 aataatggca aaagtgcggc gcttcgccac ctcttgcaaa taaggagaca acgtcataat
    30061 tctttcttct tgagtaagcg gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta
    30121 ttttacccat caacgggggg gaggcaatca gtgtttacac aggagctgct ccagttcgtg
    30181 caacgaagaa acggtccagg tgggcgcgat gccttctggt tgctcgcgat ggtgtgcatt
    30241 cagccagcag gtcgcaagcc cggcgttgat gccaccgaga atatcggact cggcagtgtc
    30301 gccaaccatc agcacgcgtg aacggtcagg attgcccgcc tgttccagcg cataatcgaa
    30361 aattttctta ttcggtttgg caacgccaac ttcttcggaa atcaccagca aatcgaagta
    30421 atcacgcagg cccgtgcgtt ccagacgcac ctgttgcaag gcactaaagc cgttggtgat
    30481 gatgccgatt ttggcgttgc cacgaatggc gttaagcaga gaaaccgcgc ccggcagcgg
    30541 cgtgcagatt tccgccatcg cattaataaa ggcttcgttg agtttacctg gctcgacgtt
    30601 cagccgttcg gcccagctct caaaccgccc gtgctgaagc tgtaatgaag tgatcgcgcc
    30661 gttttgataa tccacccaca gtggcttgtt aacggcctga tagtcctgaa aatcttcagc
    30721 ggtaaaggtg acgctgtaat caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt
    30781 aaacagcgtt tcatcggcat caaagaaaat ccagtcccac ttcattattc caccttgtct
    30841 tacatactga ttggcaacgc catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag
    30901 taattgcggc gaatcgtcgc ctcgttaaag cctaaacttt cgtacagggc aatggcggca
    30961 gcatttgagg cacggacttc cagccatagt gtcgccacgc cgcgtttttc cagttcgtcg
    31021 atcagatgtt ccagcagcgc ccttcccaat ccctgacgct gatagtcagg atcgaccgca
    31081 atattgaaca atgtagcttc atccagcacc acttgcgtaa tcgcaaacgc cgccattttg
    31141 ccgttttgcg ttaactgaaa gttgagataa cgctcgccct ggttgctggc aaaggttttt
    31201 tcactccacg gaaaggcgtg ggcgcgttgt tcaatgtggt aagccgctgg taaatcagtc
    31261 gtttcgaggg aagaaatcgt gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc
    31321 cgttgggttt gcccgtaatt cggtgagcgc cggtgatgcc acctgagcgc cttccagtga
    31381 tagcggttcg tcagtaccca accgccagct gttgcagcga ctgccttgcg gcagcatcgc
    31441 gattttttct ggcgtcagtt gcagcacttg atcggggctg acggttaatg cgcgcagaac
    31501 atcgctcact aaaggatcag tcagggcggg aagatcgttt gccaccatca ccagacggac
    31561 gtgtgccggg atcgcaatgg caatctcccc ctgcaacgcg ccagggcgac gcagcgacca
    31621 ctgggtaatg cccagttgct gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc
    31681 ctgttgtcag gggcgcaaat atagcaaatt cgtcgatacc gcgccaacat atggctataa
    31741 tcgccgccag tatcaattga ggagcattcc atgtctgcat ttaccccggc aagtgaagtc
    31801 ttgctgcgtc acagtgatga tttcgaacaa agccgtattc tgtttgccgg agacttacag
    31861 gatgacctgc ccgcgcgttt agataccgcg gccagccgtg ctcataccca gcaattccag
    31921 cattggcagg tgttaagccg ccagatgggg gataacgccc gttttagtct ggtcgccacg
    31981 gcggatgatg tcgcagattg cgatacgctg atttactact ggccgaagaa caaaccggaa
    32041 gcccagttcc agttgatgaa tttactttct ctgcttccgg tggggacgga tatttttgtt
    32101 gttggcgaga accgcagcgg cgtgcgcagc gccgagcaga tgctggctga ttatgcgccg
    32161 ttgaataaag tcgacagcgc ccgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag
    32221 ccggtatttg atgccgataa attctggggc gaatacagcg tcgatggcct gacggtcaaa
    32281 acgctgcctg gcgtgtttag ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg
    32341 acgttaaccc cgcacacgaa aggtaaagtg ctggatgtcg gctgtggcgc gggcgtgctt
    32401 tcggttgcct ttgcgcgcca ttcgccgaaa attcgtctca ccttgtgcga tgtctctgcg
    32461 ccagcggtag aagccagccg cgcaacactt gcggccaacg gtgttgaagg tgaagtcttt
    32521 gccagcaacg tcttttccga ggtgaaaggt cgttttgata tgatcatctc caacccgccg
    32581 ttccacgatg ggatgcaaac cagcctggat gcggcgcaaa cgctgattcg cggtgcggtg
    32641 cgtcatctta atagcggcgg cgagctgcga attgtagcga acgccttcct gccttatccg
    32701 gacgtgctgg atgagacatt tggcttccac gaagtgatcg cgcaaacagg gcgcttcaag
    32761 gtgtatcgcg ccattatgac ccgccaggcg aagaaaggtt aattatctcg ccgaataccg
    32821 tgtcggatgc ggcgtgaacg ccttatccga cttacaaaac ctgcacgtta gtctcctgat
    32881 ggacattttt ccagcaatta cacatcaggc gataattaac tattgacgaa aagctgaaaa
    32941 ccactagaat gcgcctccgt ggtagcaatt ctttttaaga attgatggta tgcgaaggtg
    33001 gcggaattgg tagacgcgct agcttcaggt gttagtgtcc ttacggacgt gggggttcaa
    33061 gtcccccccc tcgcaccaat tatctttact tcctttcttg tttcttccct gttttttcat
    33121 ccctgattca aattcattat catcagcatg atgccagcaa taagcgccag gcacgatggt
    33181 agcaaaatgt aatgccgcaa ctgtttgtga tacagcatca ccggcgtcaa tactaatccc
    33241 atcagaatga caatccgcca ctgatccggc gataaatccg catacatcag ccctgcaatc
    33301 agaaatcccg gcagcaacac tccccagcca ctgcccagcg tacgttgcaa catggtttta
    33361 tctctttcat tgataatgat aaccaatatc atatgataat ttttatcact tgcaagccag
    33421 agaaatccct tgctatcggg taaacctatc gctaggatta gcaatcatta tcatttagat
    33481 tactatcccg at
//
LOCUS       DBFIFS010000031        33369 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.031, whole genome shotgun sequence.
ACCESSION   DBFIFS010000031 DBFIFS010000000
VERSION     DBFIFS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.031"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(62..436)
                     /gene="tomB"
                     /locus_tag="N7A07_001352"
     CDS             complement(62..436)
                     /gene="tomB"
                     /locus_tag="N7A07_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU2476042.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(982..4131)
                     /gene="acrB"
                     /locus_tag="N7A07_001353"
     CDS             complement(982..4131)
                     /gene="acrB"
                     /locus_tag="N7A07_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU2476043.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(4154..5347)
                     /gene="acrA"
                     /locus_tag="N7A07_001354"
     CDS             complement(4154..5347)
                     /gene="acrA"
                     /locus_tag="N7A07_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU2476044.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5489..6136
                     /gene="acrR"
                     /locus_tag="N7A07_001355"
     CDS             5489..6136
                     /gene="acrR"
                     /locus_tag="N7A07_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU2476045.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            6264..9626
                     /gene="mscK"
                     /locus_tag="N7A07_001356"
     CDS             6264..9626
                     /gene="mscK"
                     /locus_tag="N7A07_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU2476046.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(9665..9829)
                     /gene="rsmS"
                     /locus_tag="N7A07_001357"
     CDS             complement(9665..9829)
                     /gene="rsmS"
                     /locus_tag="N7A07_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU2476047.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(9843..10370)
                     /gene="priC"
                     /locus_tag="N7A07_001358"
     CDS             complement(9843..10370)
                     /gene="priC"
                     /locus_tag="N7A07_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU2476048.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            10440..10817
                     /gene="ybaN"
                     /locus_tag="N7A07_001359"
     CDS             10440..10817
                     /gene="ybaN"
                     /locus_tag="N7A07_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU2476049.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     CFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10970..11521
                     /gene="apt"
                     /locus_tag="N7A07_001360"
     CDS             10970..11521
                     /gene="apt"
                     /locus_tag="N7A07_001360"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU2476050.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            11650..13581
                     /gene="dnaX"
                     /locus_tag="N7A07_001361"
     CDS             11650..13581
                     /gene="dnaX"
                     /locus_tag="N7A07_001361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU2476051.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12914..12978
                     /gene="dnaX"
                     /locus_tag="N7A07_001361"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            13634..13963
                     /gene="ybaB"
                     /locus_tag="N7A07_001362"
     CDS             13634..13963
                     /gene="ybaB"
                     /locus_tag="N7A07_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU2476052.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13963..14568
                     /gene="recR"
                     /locus_tag="N7A07_001363"
     CDS             13963..14568
                     /gene="recR"
                     /locus_tag="N7A07_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU2476053.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14678..16552
                     /gene="htpG"
                     /locus_tag="N7A07_001364"
     CDS             14678..16552
                     /gene="htpG"
                     /locus_tag="N7A07_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU2476054.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16733..17377
                     /gene="adk"
                     /locus_tag="N7A07_001365"
     CDS             16733..17377
                     /gene="adk"
                     /locus_tag="N7A07_001365"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU2476055.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            17509..18471
                     /gene="hemH"
                     /locus_tag="N7A07_001366"
     CDS             17509..18471
                     /gene="hemH"
                     /locus_tag="N7A07_001366"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU2476056.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(18468..19427)
                     /gene="aes"
                     /locus_tag="N7A07_001367"
     CDS             complement(18468..19427)
                     /gene="aes"
                     /locus_tag="N7A07_001367"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU2476057.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            19579..20883
                     /gene="gsk"
                     /locus_tag="N7A07_001368"
     CDS             19579..20883
                     /gene="gsk"
                     /locus_tag="N7A07_001368"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU2476058.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(21016..22692)
                     /gene="ybaL"
                     /locus_tag="N7A07_001369"
     CDS             complement(21016..22692)
                     /gene="ybaL"
                     /locus_tag="N7A07_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU2476059.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22929..24149)
                     /gene="fsr"
                     /locus_tag="N7A07_001370"
     CDS             complement(22929..24149)
                     /gene="fsr"
                     /locus_tag="N7A07_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU2476060.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            24367..26019
                     /gene="ushA"
                     /locus_tag="N7A07_001371"
     CDS             24367..26019
                     /gene="ushA"
                     /locus_tag="N7A07_001371"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU2476061.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(26056..26535)
                     /gene="ybaK"
                     /locus_tag="N7A07_001372"
     CDS             complement(26056..26535)
                     /gene="ybaK"
                     /locus_tag="N7A07_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU2476062.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26739..27533)
                     /gene="ybaP"
                     /locus_tag="N7A07_001373"
     CDS             complement(26739..27533)
                     /gene="ybaP"
                     /locus_tag="N7A07_001373"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU2476063.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27671..28012
                     /gene="ybaQ"
                     /locus_tag="N7A07_001374"
     CDS             27671..28012
                     /gene="ybaQ"
                     /locus_tag="N7A07_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU2476064.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(28227..30731)
                     /gene="copA"
                     /locus_tag="N7A07_001375"
     CDS             complement(28227..30731)
                     /gene="copA"
                     /locus_tag="N7A07_001375"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU2476065.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30993..31925
                     /gene="glsA"
                     /locus_tag="N7A07_001376"
     CDS             30993..31925
                     /gene="glsA"
                     /locus_tag="N7A07_001376"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU2476066.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31928..33220
                     /gene="ybaT"
                     /locus_tag="N7A07_001377"
     CDS             31928..33220
                     /gene="ybaT"
                     /locus_tag="N7A07_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU2476067.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         8141 a         8753 c         8697 g         7778 t
ORIGIN      
        1 taaataatcg gttttcgtta aaggtttttc ggacatactt ctacccatgg ttgtaataat
       61 tctaacaaga taaactcgca ggattctctt tcgtcgcatt gacaaaacaa cggaatagtc
      121 gattacctga cttccgccat ttctgaagat cctgcatatt aataccataa ctactgaaca
      181 acataaaggt gtcatccaga tattcgtcga tctgctcaat gagcttattg tcttcattat
      241 acttaatttt gtaattaagt gcgaaggtcg caatatgctc aatcagttca ttcaactgga
      301 ggttgatcgc cgaggttggg tcgttaaccc agccatgatt gctttcttca aggtttgcaa
      361 ggcagtcatg atacagagtt tcacagagaa acttaagctg tgcgatatca tgtcttttgg
      421 gtgagtattc atccataacg cgtccccttc ttagcggttg aactaacgga cacctttcgg
      481 gatggaaaaa acttactgac ctggacttgc cctctcgttt gttagcttaa ctataagcca
      541 ctctttgcag gttttcatcg cattattacg aaaaattaca attaggaagg aagcgggaca
      601 gggctcactt tttcatacta gcaccaaccc gcagcaggta aaagcagttt gctcgaactg
      661 ttcataacat ttcaggatta tcgccagatg aaattggtga cactaatacc aggattgctc
      721 tgaatatgac gtaataaccg aggaatgaat aaagaattac cgcaaataat taagaatagc
      781 ctctaaatga ttatggatta ttaaatctat tagcgctact taatataaat taactaacaa
      841 tcagtatttt ttatgaattt tatacgtggt taatactggt tttcgtatga gatcctgagt
      901 tggtggttca attactcctt aatgttcgta ggttatgcat aaaaaaggcc gcttacgcgg
      961 ccttagtgat tacacgttgt atcaatgatg atcgacagta tggttgtgct cgatatcttc
     1021 attcttgcgg ctaaagcggc ggcgaaccac cacaaagaat accggaacga agaagattgc
     1081 cagtaccgtt gcggtcacca tcccgcccat tacaccggta cctactgcgt tctgcgcgcc
     1141 ggaaccagca ccagtactga taaccagcgg cataacgccg aggataaacg ccagcgaggt
     1201 catcaggatc ggacgtaaac gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt
     1261 accttcttta tccatcaagt ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga
     1321 caacccaatg gttgtgagca ggcctacctg gaagtaaacg tcattggtca ggccacggaa
     1381 ggtggcagcc agcaacgcac cgataacccc cagcggaacg accagcataa cggagaacgg
     1441 aatcgaccag ctctcgtaca gcgccgccag acacaggaac acgacaatca acgaaatcgc
     1501 gtacagtgaa ggtgcctggt tgccggagag acgttcctga taggacatcc ccgtccagtc
     1561 atagccaaca ccggtaggca gtttgctcgc cagttgttcc atcagctcca ttgcttcacc
     1621 ggtactttta cccggtgccg cctggcctaa gatttccatg gatggcaggc cgttgtaacg
     1681 ttccagacgc ggcgaaccgt actcccaacg agaagaggag aacgccgaga atggcaccat
     1741 ctgaccatca gcagcacgaa cataccagtc gccgatatca tccggcagca tacggtattt
     1801 cgcttctgac atgacgtaaa ctttcttcac acgaccgcgg tcgataaagt cgttcacata
     1861 gctgccgccc catgcagcgc ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc
     1921 ctgcgctttt tcctggtcga tatcaatctt aaactgcggg gtatcttcca gaccgtttgg
     1981 acgtacgctg gtcaacatat cagggtgctt cgctgcttct gcaagcaact ggttacgcgc
     2041 ctgagtcagt ttttcgtgac caaggccagc ctggtcaatc agctcaaagt caaagccggt
     2101 tgcggtaccc agttccacga ttgcgggcag gttaaaggcg aaaaccatcg catctttgat
     2161 ttgcgagaaa gcgcgtgttg cacgcatggt aatcgcttca actttgtttt cttcacccgg
     2221 acgatcggcc cagtccttca aggaaacgaa cgcaataccg gtattctgac cacgtcccgc
     2281 aaagccgaag ccgttaacgg cgaacaccga ctcaacgttg ttcttttctt tggtcagata
     2341 gtaatgcgtt acctcattga gcactttctg cgtacgttcc tgcgttgcac ctgctggcag
     2401 ctgaaccatg gtcataaata cgccctggtc ctcatctggc aagaaggagc ttggcagacg
     2461 cacaaacaga taggccatgc cgaccacgat gatcagatac agcaccagat aacgccccgt
     2521 actgcgcaga ataccgccta cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg
     2581 gttaaaccag ccgaagaagc cttttttacc ttccccgtga tcgcctttgg caatcggttt
     2641 cagcatggtg gcacaaagag ctggagtcag gatcaacgcc accagtaccg acagcgccat
     2701 tgctgaaaca atggtaatag agaactgacg ataaattgcc ccagtagaac cgccaaagaa
     2761 ggccatcggt acgaataccg ccgacagtac catcgcgata ccgaccagag cgccctgaat
     2821 ctgccccatc gacttacggg tggcttcttt tggcggcaaa ccttcttccg ccatcacacg
     2881 ctcaacgttt tctaccacaa cgatagcgtc atccaccaac aggccgatgg cgagcaccat
     2941 cccgaacatt gttagcgtgt ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc
     3001 gagcaatacc accggtacgg caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa
     3061 cagatacata accaggaaca cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg
     3121 aatagagatt ttcacgaacg gcgtggtgtc gtacgggtaa acaattttca gacccgacgg
     3181 gaagaacggt tccatcttcg ccagttcagc acggattgcc gcagcggtat ccagcgcgtt
     3241 tgcaccggtc gccagcttga tccccagacc ggaagccggt tggccgttaa actctgcgat
     3301 gatgtcgtag ttctcaccac ccagctcaat cttcgccaca tcacgcagca gtacgcggga
     3361 accatcctga ttcactttca gcaggatttt gccgaactct tcagtagagg tcagacgcgt
     3421 ctgagcaata atagaggcgt taagctgttg gcctttcacc ggcggtgtac caccgagctg
     3481 gcccgccgca acctgggcgt tctgcgcttt gatggcggta ataacatcaa ccggcgttag
     3541 ctggaatttg ttcagttcat tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa
     3601 caactgaacg tcacccacgc cagacgtacg gctgatggca tctttcatat tcgccgccac
     3661 gtagtcggag atatcctcct gcgtcatggt gccatcggtg ttgataacgc cgacaaccat
     3721 caggaagctg ctggatgatt tctcaatgct caccccttgc tgctgaactt cttgcggcag
     3781 caacggcatc gccagctgca gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt
     3841 accagactca aaggtcaggg tgatctgtac ggtacccgtg gagtcactgt tagaggacat
     3901 gtacatcagg ttatcgatac cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg
     3961 cactgttttc gcatcagcgc cggggtagga ggcggagatc gttactgccg gcggtgcaat
     4021 cgtaggatat tgcgccaccg gcagtttgag gatcgccagc ccccctgcca acatgatgat
     4081 aatggcgatc acccacgcaa aaatcgggcg atcgataaag aaattaggca tgtcttaacg
     4141 gctcctgttt aagttaagac ttggactgtt caggctgagc accgcttgcg gcttgctggt
     4201 tattatcagc ggtaacttct tgtgctttta cctggacacc aggacgcact ttctgcagcc
     4261 cacttattac tacgcgatcg cctgctttca gaccttctgt caccagccac ttatcgccaa
     4321 tagcctggct tgcaacgatc ggacgggttt ccactttgtc atccgcgcca accaccagta
     4381 cggtggcatc gccacgcggc gtacgggtta cgccctgttg cgggactaaa atagcgtttg
     4441 gattaagccc ttcttccaga cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg
     4501 ggttcgggaa gatagcgcgt agggtgatag acccagtggt ctgatcaacg gtaacgtcag
     4561 agaattccag cgtaccgtcc tgcgggaact taatgccgtc actggtgatc agcgacactt
     4621 tggctttgcc gttctcttgt ttcagcgtgc cattcgccag ttcctgtttc aggcgcagga
     4681 agtcgttgct ggactgggtc acatcaacgt agatcggatc aagttgctgc acggttgcca
     4741 gcgcagtcgc ctgaccgttc tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc
     4801 gaccgctaat cggagaggtg actttggtgt aagccagatt gatccgcgca gtttcaacgg
     4861 cagctttcgc cgcagttacc gcagcattcg cctgttgcgc atcagccaga gcctgatcgt
     4921 actcttgctt actgatgtac tgagtaccga gcagtttctg ataacgattc accgtcaatt
     4981 gcgcgatatt ggctgcagcc tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg
     5041 cctgataggt cgcaggatca atctgataga gagagacacc tgcttcgatg tcgctacctt
     5101 ctttgaaatt acgcttcagg ataatcccgc taacttgagg acgaacttct gcgatccggt
     5161 aggcactggt gcgacccgga agctcggttg tgatctgcag aggttcagtt ttgactgtta
     5221 ctacgccaac ggcgggcatc tgctggccac cttgttgggc ctgtttgtcg tcacatcctg
     5281 ttagggctaa gctgcctgag agcatcagaa cgaccgccag aggcgtaaac cctctgtttt
     5341 tgttcatatg taaacctcga gtgtccgatt tcaaattggt caatggtcaa aagttaataa
     5401 acccattgct gcgtttatat tatcgtcgtg ctatggtaca tacattcaca aatgtatgta
     5461 aatctaacgc ctgtaaattc acgaacatat ggcacgaaaa accaaacaag aagcgcaaga
     5521 aacgcgccaa cacatcctcg atgtggctct acgtcttttc tcacagcagg gggtatcatc
     5581 cacctcgctg ggtgagattg caaaagcagc tggcgttacg cgcggtgcaa tctactggca
     5641 ttttaaagac aagtcggatt tgttcagtga gatctgggaa ctatcagaat ccaatattgg
     5701 tgaactagag cttgagtatc aggcaaaatt ccctggcgat ccactctcag tattaagaga
     5761 gatattaatt catgttcttg aatccacggt gacagaagaa cggcgtcgat tattgatgga
     5821 gattatattc cacaaatgcg aatttgtcgg agaaatggct gttgtgcaac aggcacaacg
     5881 taatctctgt ttggaaagtt atgaccgtat agaacaaacg ttaaaacatt gtattgaagc
     5941 gaaaatgttg cctgcggatt taatgacgcg tcgggcagca attattatgc gcggctatat
     6001 ttccggcctg atggaaaact ggctctttgc cccgcaatct tttgatctta aaaaagaagc
     6061 ccgcgattac gtcgccatct tactggagat gtatctcctg tgccccacgc ttcgtaatcc
     6121 tgccactaac gaataaccct gaatctgact ccaggatttt tcctggacat tttcgtcgtt
     6181 gctattctgg ttcactgcgt cgtgatattc ttgcggtttg actttttcag gtcgttcttc
     6241 aggttcagaa accttcattc atcatgacta tgttccagta ttacaaacga tcacggcatt
     6301 ttgttttttc agcatttatt gcttttgttt ttgtcttgtt atgccagaac acggcgtttg
     6361 cgcgggcgtc atcgaatggt gatctgccga caaaagcgga cctgcaggcg caacttgact
     6421 cactaaataa acaaaaagat ctttctgctc aggacaaact ggtgcagcag gatctgacag
     6481 atacattagc caccctcgat aaaatcgatc gcgtaaaaga agagaccgtt cagctacggc
     6541 aaaaagtcgc tgaagcgccg gaaaaaatgc gccaggcgac cgcggcgtta acagcactta
     6601 gcgatgtcga taacgacgaa gaaacgcgca aaattctgag cacgctttcg ttgcgccagc
     6661 tggaaactcg cgttgcccag gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt
     6721 cttataacag ccagctggtt tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt
     6781 ataacgcttc gcagcagctg caacaaattc gcagtcgtct ggatgggact gatgtcggcg
     6841 agacggcctt acgtcccagc cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg
     6901 cggagattga ccagcagcgt aaaagcctgg aagggaatac cgtcttgcag gataccttgc
     6961 aaaagcaacg tgattacgtg acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt
     7021 tgcaagaagc ggtaaacagc aagcgcctga ctttaaccga aaaaacggcg caggaagccg
     7081 tctccccgga tgaagccgcg cgtattcagg ctaatccgct ggtgaagcag gaactggaaa
     7141 ttaaccagca gttaagtcag cgtctgatta ccgcgactga aaacggtaat cagttgatgc
     7201 agcaaaacat taaagtcaaa aactggctgg agcgggcgct gcaatcggaa cgcaatatta
     7261 aagagcagat tgccgtcctg aagggcagcc tgctgttgtc tcgtatcctt taccagcaac
     7321 aacaaacgct gccctcggcg gatgaactgg aaaacatgac caaccgcatc gcggatttgc
     7381 gtctcgaaca gtttgaagtt aaccagcagc gtgatgcact cttccagagc gatgcgttcg
     7441 tcaacaaact ggaagaaggt cacaccaacg aagtcaacag cgaagttcac gatgcgttat
     7501 tgcaagtggt tgatatgcgt cgcgaattgc tggatcaact caacaaacag ttgggtaacc
     7561 agctgatgat ggccattaac ctgcaaatca accagcagca gttaatgagt gtgtcgaaaa
     7621 acctgaaatc catcctgact cagcaaatct tttgggtgaa cagtaaccgt ccaatggact
     7681 gggactggat caaagcgttc ccgcaaagcc tgaaagatga atttaagtcg atgaaaatta
     7741 cggttaactg ggaaaaagcc tggcccgccg tttttatcgc tttcctcgct ggtttgccgc
     7801 tgctgttgat tgccgggctg atccactggc gtctggggtg gctgaaagcg tatcaacaaa
     7861 aactggcttc cgctgtgggt tccctgcgta acgacagcca gctcaacaca ccaaaagcga
     7921 tccttatcga cctgatccgt gcactgccgg tgtgcctgat tattctcgcg gttggcctga
     7981 ttctgttgac catgcagctc aacatcagcg aactgctatg gtcgttcagc aaaaaactgg
     8041 cgattttctg gctggtgttt ggcctgtgct ggaaagtgct ggagaagaac ggcgttgccg
     8101 tgcgtcactt cggcatgccg gaacagcaga ccagccactg gcgtcggcaa attgtccgca
     8161 tcagtctcgc gttgctgcct atccatttct ggtctgtggt ggcagaactt tccccgctgc
     8221 atctgatgga tgatgtgctg gggcaggcga tgattttctt caacctgctg ctgattgcct
     8281 tcctggtatg gccaatgtgc cgcgaaagct ggcgtgataa agagtcgcac accatgcgac
     8341 tggtcaccat taccgtgctg tcgattatcc cgattgcatt gatggtgctg actgccacgg
     8401 gttatttcta caccacgctg cgcctctccg ggcgttggat tgaaaccgtt tatctggtga
     8461 tcatctggaa cttgctctac cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta
     8521 tcgcctggcg tcgtgcgctg gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg
     8581 ccgaaccgcc ggaagaaccc accattgcac tggaacaggt taaccagcag acgctgcgta
     8641 ttaccatgtt gctgatgttt gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt
     8701 tgatcaccgt gttcagctat ctcgacagca ttacgctgtg gcattacaac ggtactgaag
     8761 ctggtgctgc ggtggtgaaa aacgtcacca tgggtagtct gttgtttgcg attatcgcct
     8821 caatggtggc ctgggcgttg attcgcaacc tgcctggttt gctggaagtg ctggtgctct
     8881 cgcgactgaa tatgcgccag ggcgcgtcgt atgccattac taccatcctt aactacatca
     8941 ttattgctgt tggtgcgatg acggtgttcg gatcgctggg cgtctcttgg gataaactcc
     9001 agtggctggc cgcagcatta tccgtaggtc ttggttttgg tttacaagaa attttcggta
     9061 acttcgtctc cggtttgatc attctgttcg aacgtccggt gcgtattggc gatacggtaa
     9121 ccattggtag cttctcgggg acggtaagta aaatccgtat tcgtgcgaca acgattaccg
     9181 atttcgatcg caaagaagtg atcatcccga acaaagcgtt tgttaccgag cgtctgatca
     9241 actggtcgtt gactgacact actacgcgtc tggtgatccg tctcggcgtg gcctatggct
     9301 ccgatctgga aaaagtgcgt aaagtgttac tgaaggcggc gactgagcac ccaagggtga
     9361 tgcacgaacc aatgccggaa gtcttcttta cggcatttgg tgccagcacg ttggatcatg
     9421 agctgcgtct gtatgtgcgt gaactgcgtg accgtagtcg tactgtcgat gagctgaacc
     9481 gtactatcga tcagctgtgc cgtgaaaacg acatcaacat tgcctttaac cagcttgaag
     9541 tgcatctgca caacgagaag ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg
     9601 acgatccgac gccagcggta gggtaaggac aataaagggc gacagtgatg ccgcccttta
     9661 ttttttactc gctttgtgaa gcctcatcgc tctgtaactt cttttcataa tcacgcttta
     9721 caatatccag cgcgcgcagc acggtgcggg ctgggatctg attttcttcc agcagcacaa
     9781 tcaaatcgac agccagtttg acatcgtcag gggcattttc cagtgacata ctctctccat
     9841 tgctagcggg ttaaacgcgc taacctgttt tcgatttttt ccagcgcatg gcggcagcgt
     9901 gccaggcgcg cttcataggc ttccacttca cgatgcagcg tttgctgttc cacgagatcg
     9961 gtcacccgcg ccaggcgggc tctacgttcg gcaaccatct cacgtagccg ccgctcaaaa
    10021 tcctgatgct gaatacgttt acgctgccag cgggcaattt ggggtggtgc gctatcccac
    10081 tcgcgtagcg accaggctgt ggcttcacgc gctatagcct ccagttgcgc cgccagatgt
    10141 tctgccagcc aggcgacttg cggcagttgc tgttgctcaa ctgcatgacg aagcgcagcc
    10201 agattatcgc ccgccttgtc gagacaagcc tgtagtgttg tcgcacgagt ctgaaaaaga
    10261 tgcctgttga aacgagcact tagcgtggcg aactgtgaca ccggggcaca acgctgacgc
    10321 agcgtagcga gctgtccttc cagtttttcc agcagcaggg cggttttcac gataagacct
    10381 caatgaaaat gataattgtt atgctaaagt agccactctg taagctgaca acactcaata
    10441 tgcaacgaat cattttaatc atcattggct ggctggcggt agtgctgggc acgctgggcg
    10501 tggtattacc ggtattaccg acgacgccgt ttatcctgct ggcggcctgg tgctttgccc
    10561 gttcttcccc gtgctttcac gcctggttgc tgtaccgctc atggtttggc agctatctac
    10621 gtttctggca gaaacatcat gcgatgccgc gcggcgtcaa accgcgggcg attttgctta
    10681 ttttgctcac gtttgccatt tctctgtggt tcgtccagat gccatgggtg cgcatcatgt
    10741 tgctggtaat tctcgcctgt ttgcttttct atatgtggcg aattccggtg attgatgaaa
    10801 agcaagaaaa gcactgaagc acaacaatcg cagttgcaat tattgcgtac agccagtaca
    10861 ttctggcgtt ttcgagcaca ggcgcaggcg gtcaaaggtt aaacaactgt tacttttgat
    10921 acgtttaaaa cgcgccgtga gtaccaccgt aacaagcagg catacactta tgaccgcgac
    10981 tgcacagcag cttgagtatc tcaaaaatag catcaaaagc attcaggact acccaaaacc
    11041 cggcattctt ttccgcgatg tcaccagctt actggaagac ccgaaagctt acgctctcag
    11101 catcgacttg ctggttgagc gttacaaaaa tgcgggcatt aacaaagttg tcggcaccga
    11161 agcgcgtggc ttcttgtttg gcgctccggt agctctgggt ctgggcgttg gctttgtacc
    11221 ggtccgtaaa ccgggcaaac tgccgcgtga aaccatcagt gaaacttacg atctggaata
    11281 cggcaccgat cagctggaga tccacgttga tgccatcaaa ccgggcgaca aagttctggt
    11341 ggtggacgat ctgctggcaa ccggcggcac tatcgaagcg accgttaaac tgatccgtcg
    11401 tctgggtggt gaagtggctg acgctgcgtt cattatcaac ctgttcgatc tcggcggcga
    11461 acagcgtctc gaaaaacagg gcattaccag ctacagcctt gtcccgttcc cgggccatta
    11521 attatcgcca gtcttgtgct gcccacgcta cggacagcac aagatgtgca ttcagcctcg
    11581 ccgttctgac ggggctgtgt tagcattacc ccttcgagaa tccaccttcc agcgtttcag
    11641 agcctgccaa tgagttatca ggtcttagcc cgaaaatggc gcccacaaac ctttgctgac
    11701 gtcgtcggcc aggaacatgt gctgaccgca ctggcgaacg gcttgtcgtt agggcgtatt
    11761 catcatgctt atcttttttc cggcacccgt ggcgtcggaa aaacctctat cgcccgactg
    11821 ctggcgaagg ggctaaactg cgaaaccggc attaccgcga cgccgtgcgg cgtgtgcgat
    11881 aactgtcgtg aaatcgagca ggggcgcttt gtcgatctga ttgaaatcga cgccgcctcg
    11941 cgcaccaaag ttgaagatac ccgcgacctg ctggataacg tccagtacgc tccggcgcgt
    12001 ggtcgtttca aagtttatct catcgacgaa gtgcatatgc tgtcgcgcca cagctttaac
    12061 gcactgttaa aaacccttga agagccgccg gagcacgtta agtttctgct ggcgacgacc
    12121 gatccacaga aattgccggt gacgattttg tcgcgctgtt tgcaatttca tctcaaggcg
    12181 ctggatgtcg agcaaattcg tcatcaactt gagcacatcc tcaatgaaga acatatcgct
    12241 cacgagccgc gggcgctgca attgctggcg cgcgccgctg aaggcagcct gcgagatgcc
    12301 ttaagtctga ccgaccaggc gattgccagc ggtgacggcc aggtttcaac ccaggcggtc
    12361 agtgcgatgc tgggtacgct tgacgacgat caggcgctgt cgctggttga agcgatggtc
    12421 gaggccaacg gcgagcgcgt aatggcgctg attaatgaag ccgctgcccg tggtatcgag
    12481 tgggaagcgt tgctggtgga aatgctcggc ctgttgcatc gtattgcgat ggtacaactt
    12541 tcgcctgctg cacttggcaa cgacatggcc gccatcgagc tgcggatgcg tgaactggcg
    12601 cgcaccatac cgccgacgga tattcagctt tactatcaga cgctgttgat tggtcgcaaa
    12661 gaattaccgt atgcgccgga ccgccgcatg ggcgttgaga tgacgctgct gcgcgcgctg
    12721 gcgttccatc cgcgtatgcc gctgcctgag ccagaagtgc cacgccagtc ctttgcaccc
    12781 gtcgcgccaa cggcagtaat gacgccaacc caggtgccgc cgcaaccgca atcagcgccg
    12841 cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc ggcgcgccag
    12901 cagttgcagc gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaaccggc agccgctacc
    12961 cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac cgatcgcgtt
    13021 caggcgcgtc cggtgccatc ggcgctggaa aaagcgccag ctaaaaaaga agcgtatcgc
    13081 tggaaggcga ccactccagt gatgcagcaa aaagaagtgg tcgccacacc gaaggcgctg
    13141 aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctggc ggcagaagcc
    13201 attgagcgcg acgcgtgggc ggctcaggtg agtcaacttt cgctaccaaa actggtcgaa
    13261 caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct gcatttgcgc
    13321 tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga agcgttgagc
    13381 atgttaaaag gttcaacggt tgaactgact atagttgaag atgataatcc cgcggtgcgt
    13441 acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc gcgcgagtcc
    13501 attattgcgg ataataatat tcagaccctg cgtcggttct tcgatgcgga gctggatgaa
    13561 gacagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct taacgtgatt
    13621 gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc aagcccagca
    13681 gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag ttaccggcga
    13741 atctggcgca ggtctggtaa aagtgaccat caacggtgca cataactgcc gtcgcgtaga
    13801 gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg tggctgcagc
    13861 attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg cttctgtatc
    13921 ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac cagcccgctg
    13981 ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa gtcggcgcag
    14041 cgtatggcgt tcacactgct tcagcgcgat cgtagcggcg ggatgcgtct ggcgcaggcg
    14101 ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacctt caccgaacag
    14161 gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat ctgcgtggtg
    14221 gagagtccgg cggacattta cgccattgag cagacggggc agttttcagg tcgttatttt
    14281 gtgttgatgg gacatctgtc accgctggac ggcatcggtc cggatgatat cggccttgat
    14341 cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc caccaaccct
    14401 acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca atatgacgtg
    14461 gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat ggtcgatggc
    14521 accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc aaacgagagc
    14581 aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc ctcatcctgt
    14641 ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag aaactcgtgg
    14701 ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct attccaataa
    14761 agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca agttgcgttt
    14821 ccgtgcgctc tctaacccgg acctgtacga aggtgatggc gaactgcgcg ttcgtgtctc
    14881 tttcgataaa gacaagcgca cgctgaccat ctccgataac ggcgtgggga tgacccgcga
    14941 cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat tcctcgaatc
    15001 cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg ttggtttcta
    15061 ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag gcgaaaaacc
    15121 agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg ccgacatcac
    15181 caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag acgagttcct
    15241 cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg cgctgccggt
    15301 agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg agaaaattaa
    15361 caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag agtacaaaga
    15421 gttctacaaa cacatcgccc acgattttaa cgatccgctg acctggagcc acaaccgtgt
    15481 tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc cgtgggatat
    15541 gtggaaccgc gatcataaac acggcctgaa actgtatgtt cagcgtgtgt tcatcatgga
    15601 cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga ttgactccag
    15661 cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa cgcgtaacct
    15721 gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga aagacgacgc
    15781 ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag gtccggcgga
    15841 agatttcgct aaccaggaag cgatcgccaa attgctgcgt tttgcttcta cccataccga
    15901 ttcttctgcg cagaccgtgt ctctggaaga ctacgtttcc cgcatgaaag aagggcagga
    15961 gaaaatctac tacatcactg cagacagcta tgcggcagcg aagagcagcc cgcacctgga
    16021 actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg atgagtggat
    16081 gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta aagttgacga
    16141 gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg agaaagcact
    16201 gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag atgtccgtct
    16261 gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg aaatgagcac
    16321 tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga aatacatctt
    16381 cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag atgaagcgaa
    16441 gttcagcgag tgggtagaac tgctgctgga tcaggcactg ctggcagaac gcggcacgct
    16501 ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct gatgtaatgc
    16561 cggatgacct tcgtgtcatc cggcattttt cttttcatta tctgcacttt ccgcaaatta
    16621 tctcgccatt aaccgtttca gcccctggtg cctttcttga ggcaatcgcc tgatggtggt
    16681 atcgtttatc gctttttcaa aaaattcgac acattttaag gggattttcg caatgcgtat
    16741 cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca tcatggagaa
    16801 atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca aatctggctc
    16861 cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca ccgacgaact
    16921 ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg gtttcctgtt
    16981 ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg gcatcaatgt
    17041 tgattacgtt ctggaattcg acgtaccgga cgaactgatc gttgaccgta tcgtcggtcg
    17101 ccgcgttcac gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc cgaaagtaga
    17161 aggtaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc aggaagaaac
    17221 cgtacgtaaa cgtctggttg aataccatca gatgacagca ccgctgatcg gctactactc
    17281 caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca agccggttgc
    17341 tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca gatctgcttt
    17401 cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt tcttcttcct
    17461 cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat gcgtcagact
    17521 aaaaccggta tcctgctggc aaacctgggt acgcccgatg cccccacacc tgaagcggta
    17581 aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc acggttgtta
    17641 tggtggccat tgctgcgcgg cgtgattttg ccgctgcgct cgccgcgtgt ggcgaagctg
    17701 tatgcctctg tctggatgga aggtggctcg ccgctgatgg tttacagccg ccagcaacag
    17761 caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag ctacggctcg
    17821 ccatcactgg aaagcgccgt agatgaactc ctggcagagc atgtagatca tattgtggtg
    17881 ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga tgaactggca
    17941 cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttatacgtga ttacgccgat
    18001 aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc caaacatggc
    18061 gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc agatgaaggc
    18121 gatgattacc cgcaacgttg ccgcacaacg actcgtgaac tggcttccgc attagggatg
    18181 gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gtcgggaacc ctggctgatg
    18241 ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca tattcaggtg
    18301 atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc cgagcaaaac
    18361 cgtgaggtct tcctcggtgc cggcgggaaa aaatatgaat atattccggc gcttaatgcc
    18421 acaccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta aagctgagcg
    18481 gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat ccgtgaataa
    18541 tgcaaaaagg cgtgcagcgt gcctgggtag agtttgaact cacagggctg ctgatgcgcc
    18601 gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc cgccccggca
    18661 ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta atacggcgac
    18721 tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc ctgttgcgtt
    18781 aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa tccgtaaagc
    18841 ccataccaca gcaaaacgcc cgcaacttta ccgcaatcga tctgtttatc acgcaaccac
    18901 aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc aatgcgggac
    18961 atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc cacaatttcc
    19021 tctatcactt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc aatcaccgta
    19081 cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag attgccgaga
    19141 ataaaaccgc ctccatgcaa ataaaatagc gttgccgggc tatctggctg cggacaaaag
    19201 agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctttggt tgccatttct
    19261 ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg ctcagcaatc
    19321 gttcccgttg cgggccaggg cggtaaatcc ggctgaagag tattcacaac ggtcttcatt
    19381 tcagcagaaa taaggtccag aacaggtaat ttgttttccg gcttcataca aaaactcctt
    19441 tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc tgctgatatt
    19501 gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc gctcagttaa
    19561 ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca ttacttcccc
    19621 gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac cagcgccgcc
    19681 tgggtagtgg gtatcgatca aacgctggtc gatattgaag cgaaagtgga tgatgaattc
    19741 attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga tgtagccgaa
    19801 gcgctttatc aggaactaaa acagaaaaac ctgattaccc atcagtttgc gggtggcacc
    19861 attggtaaca ccatgcataa ctactcggtg ctcgcggacg accgttcggt gctgctgggc
    19921 gtcatgtgca gcaatattga aattggcagc tatgcctatc gttacctgtg taacacctcc
    19981 agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg ttgctttacg
    20041 ctgattggcg agtccgggga acgtaccttt gctatcagcc ctggccacat gaaccagctg
    20101 cgggctgaaa gtattccgga agatgtgatt gccggagcct cggctctggt tctcacctct
    20161 tatctggtgc gttgcaagcc gggtgaaccc atgccggaag caaccatgaa agccattgag
    20221 tacgcgaaga aatataacgt accggtggtg ctgacgctgg gaactaagtt tgtcattgcc
    20281 gagaatccac agtggtggca gcaattcctc aaagaccacg tctctatcct tgcgatgaac
    20341 gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc tgacaaggcg
    20401 ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta tatggcgggc
    20461 tttaccgaag acgaagcgaa acgtaaaacc cagcatccgt tgctgccggg cgctatagcc
    20521 gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgtca gaatccgctg
    20581 cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat gaacaccaac
    20641 ggagcagggg atggcgcact ggcagcgttg ctgcatgaca ttaccgccaa cagctaccat
    20701 cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta ttcatcgtta
    20761 gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca gcattcacct
    20821 cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta ctgggatcgt
    20881 taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg cattttgctc
    20941 tcaatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc atattttaca
    21001 tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt ccagcagttc
    21061 cagcatagta cgggcaattt cacgctcgcc catcactact tgattcgcac cacgttcggt
    21121 gatatacgcc acttcatcgt cataatgggc gcgggcaata atctcaatat ccggattttt
    21181 cgcgcgggca gatgccacaa tctcacccgc ttcataaccg ttggggatcg tcaggatcag
    21241 ccattttgca cattccagat gcgccagttg cataatttct tcgttcgccg cattgcccaa
    21301 tactgcgcgg accccgcgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac
    21361 caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta
    21421 acccaccaat agcgcatggt tgcaaatatc cactgggatc tgcttctctt cttcgattgc
    21481 ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagtagtgc
    21541 gaacagtact gggttgagca taatcgacag gatcgcccct gccagtacca ggttttgtcc
    21601 ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg cgaactcacc
    21661 aatctgcgcc aggctggcgg cgatagttaa tgccgtacgt tgggagtgac caaacagtcg
    21721 caccaggaaa aacgcggcta acgacttacc aaacagaata atcgccagcg tcgccagcac
    21781 tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa
    21841 cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc
    21901 agactcgttc agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga catcaaacag
    21961 ctctaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaacagctc
    22021 gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag
    22081 catcataatg gcgataaacg cgatcacttt gccgatggtg atccccatat cgactgcaag
    22141 agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag
    22201 cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg
    22261 actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga
    22321 aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg cagagagcgc
    22381 catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt
    22441 taccgccatc aaatccttga gcgaaaagtg cagacccacg ccaaacatca acagaatgac
    22501 gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg
    22561 tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc
    22621 cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt
    22681 ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac
    22741 caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga
    22801 attttgcaga aaaatggaat tagctgcaaa aaagcacgga tcggaaaaca gaaggcggtt
    22861 taacagagga aaggcttatg gcgactgtaa aacgatgcag ccaaagtttg cctttggctg
    22921 caatgaaatc agtctttatg ccggttatca ggcaggaata tggtcaacat ccccaatagt
    22981 ggcaggaaag cacatatttt atagactaac tcgatgctgg tgtgatcggc gataagcccc
    23041 agaactgccg ctcccagacc tcccatgccg aaagcaaaac cgaaaaagag tccagaaacc
    23101 ataccgatac gtcctggaag cagctcctga gcgtagacca gaatggcaga gaatgccgaa
    23161 gcgaggataa atccaataat caccgttaaa acccccgtcc agtgcaggct ggcgtagggt
    23221 aaaatcagcg taaacggcgc aacgccgagg atagagcccc aaatcacata tttccgccca
    23281 attttatccc ctacaggccc gccgatcacc gtacctgccg caacggcaaa caggaaggca
    23341 aacagatgaa gctgagcatt ctggatagat aatccgaatt tttgcatcag ataaaaggtg
    23401 taatagctgc tgatgctcgc catatagaaa tatttcgaga aaatgaggat taacagaatg
    23461 ctgaccgcca gtaccacttt gttgcgcggc agtggattga taatcgtcgc tttgggtttt
    23521 cctttattca ttcggtgctg tgccgagtac caacggctga tttgcgccaa caccacgatc
    23581 gccagcagtg ccgcaagcac aaaccaggca acgttgcctt tgccataagg cgcgataatc
    23641 accgccgcca gcaagggtcc cagggaactg ccaaagttgc cgccgacctg aaagatagat
    23701 tgtgccaggc catgccgccc gccggaagcc atacgggcca cgcgagaaga ttccggatga
    23761 aagaccgatg aaccggtacc gaccagcgcc gccgccagca gaactgcgcc aaaactgccc
    23821 gccagcgcaa gcagcaccag accacttaag gtaaagcaca tgccaattgg caacgaccac
    23881 ggcatcggat atttatcggt ccagtagccg accactggtt gcagtagcga agaggcgagc
    23941 tggaaggtga gggttatcat gccaatctgc ataaatgtca gagaaaattc tgactgaagc
    24001 agaggataaa tcgccagaat cagcgattgg atcatgtcgt tcagcagatg tgagaggctg
    24061 atagcaccta aaataccaaa cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc
    24121 acaggctggg tttgttcact cattgccata ggaaagtcac tttttcaggg ttgcgatgtt
    24181 aagaatgatc ttatttgtga ttattacccg actaacatac ctgtatgcgc cgcctgaagg
    24241 aagtctcaac gctgaataca gaatttctaa tctggatgca gatttatctt caccggacgc
    24301 agacttgtct atgatgtcgc gtcatactat ttttcaacac gttgaaatca ggtcagggag
    24361 agaagtatga aattattgca gcggggcgtg gcgttagcac tgttaaccac atttacactg
    24421 gcgagtgaag ctgctctggc gtatgagcag gataaaacct acaaaattac agttctgcat
    24481 accaatgatc atcatgggca tttttggcgc aatgaatatg gcgaatatgg tctggcggcg
    24541 caaaaaacgc tggtggatgg tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg
    24601 ctactttccg gtggcgacat taacactggc gtgcccgagt ctgacttaca ggatgccgaa
    24661 cctgattttc gcggtatgaa tctggtgggc tatgacgcga tggcgatcgg taatcatgaa
    24721 tttgataacc cgctcaccgt attacgccag caggaaaagt gggcaaagtt cccgttactt
    24781 tctgccaata tctaccagaa aagtaccggc gagcgcctgt ttaaaccatg ggcgctgttt
    24841 aagcgtcagg atctgaaaat tgccgttatt ggtctgacga cggatgacac agcgaagtta
    24901 ggcaatccag aaaacttcac cgatattgag ttccgtaagc ccgccgatga agcgaagctg
    24961 gtgattcagg agctgcaaca gacagaaaag ccagacatta ttatcgcggc gacacatatg
    25021 gggcattacg ataacggcga tcacggttct aacgcaccgg gcgatgtgga gatggcacgc
    25081 gcgttgcctg ccggatcgct ggcgatgatt gtcggcggtc actcgcaaga tccagtctgt
    25141 atggcagcgg aaaataaaaa gcaggtagat tacgttccag gtacgccatg caaaccggat
    25201 caacaaaacg gcatctggat tgtacaggcg catgagtggg gcaagtacgt tggtcgggcc
    25261 gactttgagt ttcgcaacgg cgaaatgaaa atggttaact accagctgat tccggtgaac
    25321 ctgaagaaga aagtgacctg ggaagacggg aaaagcgagc gcgtgctgta cacccctgaa
    25381 atcgctgaaa accagcaaat gatctcgctg ttatcgccgt tccagaacaa aggcaaagcg
    25441 cagctggaag tgaaaatagg cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg
    25501 cgttttgtac agaccaatat ggggcggttg attctggcag cacaaatgga tcgcactggt
    25561 gccgactttg cggtgatgag cggaggcgga attcgtgatt ctatcgaagc aggcgatatc
    25621 agctataaaa acgtgctgaa agtgcagcca ttcggcaatg tggtggtgta tgccgacatg
    25681 accggtaaag aggtgattga ttacctgacc gccgtcgcgc agatgaagcc agattcaggt
    25741 gcctacccgc aatttgccaa cgttagcttt gtggcgaaag acggcaaact gaacgacctt
    25801 aaaatcaaag gcgaaccggt cgatccagcg aaaacttacc gtatggcgac attaaacttc
    25861 aatgccaccg gcggtgatgg atatccgcgc cttgataaca aaccgggcta tgtgaatacc
    25921 ggctttattg atgccgaagt gctgaaagcg tatatccaga aaagctcgcc gctggatgtg
    25981 agtgtttatg aaccgaaagg tgaggtgagc tggcagtaat ccgaaagtgc cggatgttcg
    26041 catccggcac aatgcttaat cgcggcgggc gatatcagca aatttggcat cgaggatctt
    26101 cgccagatcg cctgccgcca gttcgatatc cagtccgcgc ttgccgccgg aaacataaat
    26161 agtggcaaat tcttgtgcgg gggcgtcgat aatcgttggc agacgttttt tctgccccag
    26221 tgggctaatc cccccaacca ggtatcccgt cgaacgctgc gcgaccatcg gatcggccat
    26281 ctcaactttc ttggcaccca gcgcttttgc tactttttta aggtccagtt gacctgcgac
    26341 cggcgtaacg gccacggcaa ggtgtttcat atcaccgttc actgcgacca gcagcgtttt
    26401 gtagacctga tccggattca aacctaattt tttgacgact tcatcgccaa aattggtttc
    26461 agccggatcg tgctcgtagg tatggatttg aaacgaaatc ttgttttttt cgagtgattt
    26521 aactgcgggt gtcatggtaa ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc
    26581 tgatggcggt ccaaaaaaaa gagtcatctt gcctaagagt attggcagga tggtgagatt
    26641 gagcgacaat cgagttacac cgtcgcttaa agtgacggca taataataaa aaaatgaaat
    26701 tcctctttga cgggccaata gcgatattgg ccattttttt agcgcaacat ttgcggtaaa
    26761 ttcccttctc catacaggtg tagtgcaccg accgcgacca catatcgccc cggcggcatg
    26821 gcgcgtaatt tatcccgcca ggcgagattt cgctgatgca tcagcacatc gtacagcgac
    26881 tgactgaacg tattgggcag cgttatttca ttattttgcg gcggcgcatt cagccaccag
    26941 ctcatcattt gttgcagcaa ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc
    27001 agcgccagtc ctttgtcagg gagctggagc aacatggcaa tctggttttc cgccccttcc
    27061 agttcaatca cgggtttatg ttgttgcttc gccgcctgca atagctgata atcgataccg
    27121 tattctgccc gcagccccag tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc
    27181 cacagcggtt gggtagaaaa gagtgaggga gaaatgccca tctcctggct aatgtgctgc
    27241 agtttttgga gttgctcctc gctaatgcgc tcttccagcg cctcgcaggc aggcaaatta
    27301 gcaaaaggcg tatcgctggt ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg
    27361 agctttttga gcaaacgggt gggtagggga gccatatcgt ggctacccat atgaatactg
    27421 ccaatcagat gaaaatggcg attgccggga agagagatat caatggcagg ccaggtgtaa
    27481 tgattaccgc gcagcgcggc ccaaagcgtt tttacccggt acaacagatc catacgacct
    27541 ccctttgtga aatatcatgc tagcgcgcgg ggagggatgg cgcaaatgga gttggggact
    27601 tgtttaatgt gtttgtatga ttcagtatgt tcttgtatcg ctatttacag ggaagcaaca
    27661 gttaaaaact atgaaacagg caacaagaaa accgacgaca cccggagata ttcttctcta
    27721 tgaatatctg gaaccgctag atttgaaaat caatgagtta gcagagttgc tgcatgttca
    27781 tcgtaatagc gtcagtgcac tgatcaataa caatcgtaaa ctcaccactg agatggcatt
    27841 ccgtctggcg aaagtttttg ataccacagt cgatttttgg ctaaacctcc aggcggcagt
    27901 tgatctttgg gaagttgaaa acaacatgcg cacccaggaa gaattgggac ggattgaaac
    27961 agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa
    28021 catcgcttca tctcgtaggc cggataaggc gttcacgccg catccggcat ccgagcacca
    28081 ttgcctgatg cgacgcttac gcgtcttatc aggcctacaa acctgtgcct gaaccgtagg
    28141 ccggataagg cgtttacgcc gcatccggca gtcatgcgtc gatgccaaat gcgccaccct
    28201 aaagcagcgc atccgcaatg acgtacttat tccttcggtt taaaccgcag caaccggttg
    28261 gcgttactca ctacggtaat cgacgagagc gccattgccg ctccggcaac taccgggtta
    28321 agcagtgttc cagtaaacgg ccacaaaata ccggcggcga ccggaatacc gatactgttg
    28381 tagataaacg cgccgagcag gttctgcttc atgttgcgca gcgttgcgcg ggaaatagcg
    28441 agcgcatccg caacgcccat caggctatgg cgcatcaggg taatcgccgc ggtttcaatg
    28501 gcaacatcac tgccgccgcc cattgcaatg ccgacatccg cctgggccag cgctggcgca
    28561 tcgttaatgc cgtcgcccac cattgccacc tgacgtcctt cactttgcag gcgtttaatc
    28621 gcttcggctt taccgtccgg cagcaccccg gcgatcacct catcaatccc tgcttctttg
    28681 gcgatcgcat tggcggtggt tgggttatcc ccggtcaaca tcaccagacg atatcccgct
    28741 ttatgcagac gttgcagcgc cgccacgcta tcactacgca acggatcgcg tacagccagc
    28801 agggctaccg ctttcccgtc aaccgccagc agcacaggcg ttgccccttg cgatgcctga
    28861 gctgaaatat ctgcttcaat cgctttagta tcaacttgct ggtcatttaa cagcgcctga
    28921 ttgcccagca ataacgcatg accttcagct tcaccgctca cgcccagccc gcgcaatgtg
    28981 cggaaaccgt tgacctgcgg cagctgcata tcgcttgctt tatcgaggat cgcccgcgcc
    29041 agcggatggc tggaaccttg ttccagtgcc gccgccagac gcagcgcctg cgcttcgtca
    29101 aaatcagcaa atgttttcac tgcgacaacc tgcggcttcc cttcggtcag cgtcccggtt
    29161 ttatcgaaca ctacggtatc gagtgtacta gcgcgttgca gcgcgtcggc gtcccgcacc
    29221 agcacgccaa actcagccgc ccgcccgacg ccggaaataa tcgacatcgg cgtcgccagc
    29281 cccagcgcac acggacaggc aataatcagc accgtggtgg caatcaccag ggtatagaca
    29341 atctgcggtg ccggaccaaa gaaataccag attgccgcac tgacaagcgc aataaccact
    29401 actaccggca caaatacggc tgagatttta tccgccagct gaccgatttc tggcttgctg
    29461 ctctgggcct ggcgcaccat acgaataatc cttgatagcg tggtatggct gccaaccgca
    29521 ctggcgcgaa acagaacgct accgtcctgt accactgtcc cggcatggac gctatcgcct
    29581 tcgccttttt gctgcgggat aggttcgccc gtcagcatcg cttcatccag ccatgcttcg
    29641 ccctgggtaa tctcgccatc tactggcacg cggtcgcccg tcgtcaggcg cagtagcatg
    29701 cctggctgaa cttccgccag cggcacgctt ttttcacctt cgtcagtaac caggcgcgcc
    29761 gtcggcggag ttaaatcgag taatttctcc agcgcctttg aagaacgctg acgtgcgcgc
    29821 gcttccagca tatgaccgag attaatcaga ccgataatca tcgcactggc ttcatagtaa
    29881 agatgccgcg cttccatcgg gaaccactgc ggccacaggt tgacgctcat cgaatagagc
    29941 cacgccacac ccgtacccag cgccaccagc gtatccatcg tcgccgcacc gttcagcagg
    30001 cttttccatg cactgcggta aaaatggccg ccggcgaaaa ccatcactgc cagggttatc
    30061 aggccgataa ccaaccacag gctgcggttg tcagcggtga ccatcatgtt atcgccgatc
    30121 atcccccaga ccattaccgg gatacccaca gccagtgcaa caattgcctg ccagcggaag
    30181 cgcttcatcg tagcgacggc ggtttcttgc tggcgctcgc ggcgtttcgc gtcatcttca
    30241 atcgcttccg cgccgtagcc cgctttttcc accgcctgca ctaaatcttg tggggaggca
    30301 ctgcccatca ccagcgcagt acgctccgcc aggtttaccc gtgcctgagt gacgcccggt
    30361 acgctttgca gcgcattttg tacgcgggtg acacagctgg cgcagctcat gccgctcagc
    30421 agcaactgct ggctgtcatc gtcatcggcg gtcgctgccg gaagcgcctc agaaaccgct
    30481 gtcagtgctt ccgacgggat tgatgactcc gccagcggtt tagcctttgg gtggcttaca
    30541 gatgcgtcat aacccgcttg tttgatggtt tcgatcagct gttctgcact ggcagtcccg
    30601 gtaacgtgcg cttcagtgat agacacatcc gcttgttcca catccgggcg ctgttcgaga
    30661 ctttctttca cgcgtttaac gcagtgaccg caggacaggc cgtccagggt caggtcgata
    30721 gtttgtgaca taaaacactc ctttaagaca gttttgactg gctgtgataa aggttaaacc
    30781 ttccagcaag gggaaggtca agaaattaat aacccaggcg agtaaaagtc cgtaaagatt
    30841 aaaaaatcgg ctcgatttgc atcaggatta gacatttatc tctttgtttt cctgtagtta
    30901 agttgcgggt gctaagttaa atcaggatgc ctgaaaatcg gcaccggggt gaggaattac
    30961 ctcccgcatc tataaaaagg agttaacaaa agatgttaga tgcaaacaaa ttacagcagg
    31021 cagtggatca ggcttacacc caatttcact cacttaacgg cggacaaaat gccgattaca
    31081 ttccctttct ggcgaatgtg cctggtcaac tggcagcagt ggctatcgtg accagcgatg
    31141 gcaacgtcta tagtgcgggt gacagtgatt accgctttgc gctggaatcc atctcgaaag
    31201 tctgtacgtt agcccttgcg ttagaagatg tcggcccgca ggcggtacag gacaaaattg
    31261 gcgctgaccc gaccggattg ccctttaact cagttatcgc cttagagttg catggcggca
    31321 aaccgctgtc gccactggta aatgctggcg ctattgccac caccagcctg attaacgctg
    31381 aaaatgccga acaacgctgg cagcgaattt tacatatcca acagcaactg gctggtgagc
    31441 tggtagcgct ctctgacgaa gtcaaccagt cggaacaaac aaccaacttc cataaccggg
    31501 ccattgcctg gctgctgtac tccgccggat atctctattg tgatgcaatg gaagcctgtg
    31561 acgtgtacac ccgtcagtgc tccacgctca tcaatactgt tgaactggca acgcttggcg
    31621 cgacgctggc ggcgggtggt gtgaatccgt tgacgcataa acgcgttctt caggccgaca
    31681 acgtgccgta cattctggcc gaaatgatga tggaagggct gtatggtcgc tccggtgact
    31741 gggcgtatcg tgttggttta ccgggcaaaa gcggtgtagg tggcggtatt ctggcggtcg
    31801 tccctggagt gatgggaatt gccgcgttct caccaccgct ggacgaagaa ggcaacagtg
    31861 ttcgcggtca aaaaatggtg gcatcggtcg ctaagcaact cggctataac gtgtttaagg
    31921 gctgatcatg atgaacacgg aaggtaataa cggtaacaaa cccctcggtc tatggaacgt
    31981 cgtttccatc ggcattgggg caatggtggg ggcggggatc ttcgcgctgc tggggcaggc
    32041 tgcgttgcta atggaagcct cgacctgggt cgcctttgct tttggcggta ttgtggcgat
    32101 gttttccggt tatgcctatg cgcgtctggg ggcgagctat cccagcaatg gcggcattat
    32161 cgacttcttt cgtcgcggat taggcaacgg cgtcttttcg ctggcgctct cgttactgta
    32221 cctgttgacg ctggcggtga gcatcgccat ggtcgcccgt gcttttggcg cttatgccgt
    32281 gcagtttttg catgaaggca gccaggagga gcaccttatt ttgctctacg cgttggggat
    32341 cattgcggtg atgacgcttt tcaactcctt aagcaaccat gcggtagggc ggctggaggt
    32401 gatcctcgtc ggcattaaaa tgatgatcct gttattgctg attattgccg gtgtctggtc
    32461 gctgcaaccg gcgcatattt ccgtctctgc gccccccagc tccggtgcgt tcttctcctg
    32521 tatcgggata accttccttg cctatgcggg ctttggcatg atggcgaacg cggcggataa
    32581 agtgaaagat ccgcaggtca ttatgccacg ggcgtttctg gtggcaattg gcgttaccac
    32641 gttgctttat atctcgctgg cgctggtttt gcttagcgat gtatcggcat tagagttaga
    32701 aaaatatgcc gataccgccg tagcgcaggc tgcttctccg ctgctcgggc atgtgggtta
    32761 tgtgatcgtc gtcatcggcg ctttactggc gacggcttca gccatcaacg cgaacctgtt
    32821 cgccgtgttt aacatcatgg acaacatggg cagcgaacgc gaactgccga agctaatgaa
    32881 taaatccctg tggcggcaga gtacctgggg caacatcatt gtcgtggtgt tgattatgct
    32941 gatgacggcg gcactaaatt taggctcact cgccagcgtt gccagcgcca cctttttgat
    33001 ttgctatctg gcggtgtttg tggtggcgat ccgcctgcgt catgatattc acgcctcgtt
    33061 gccgattctt atcgtaggta cgttggtgat gttgttggtg atcgttggct ttatctacag
    33121 cctgtggtcc cagggcagcc gtgcgttgat atggattatt ggtgcaatct tactcagcct
    33181 tattgtggca atggtcatga agcgcaataa aaccgtataa cacctctctg cgcgcagtac
    33241 tgcctgtact gcgcctttgt ctttaattca aattaccttt cttattcctt taataaataa
    33301 gtcacaaaaa aatgatatat acgcgataaa tctgtgatgc taatcactta ttaccgtgga
    33361 ttattttta
//
LOCUS       DBFIFS010000032        32146 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.032, whole genome shotgun sequence.
ACCESSION   DBFIFS010000032 DBFIFS010000000
VERSION     DBFIFS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.032"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..282
                     /locus_tag="N7A07_001378"
     CDS             <1..282
                     /locus_tag="N7A07_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476068.1"
                     /translation="WQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTTTLNLRS
                     IGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(329..1216)
                     /gene="rarD"
                     /locus_tag="N7A07_001379"
     CDS             complement(329..1216)
                     /gene="rarD"
                     /locus_tag="N7A07_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU2476069.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPTDEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1268..1735)
                     /gene="yigI"
                     /locus_tag="N7A07_001380"
     CDS             complement(1268..1735)
                     /gene="yigI"
                     /locus_tag="N7A07_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU2476070.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1900..2769
                     /gene="pldA"
                     /locus_tag="N7A07_001381"
     CDS             1900..2769
                     /gene="pldA"
                     /locus_tag="N7A07_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU2476071.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2902..4731
                     /gene="recQ"
                     /locus_tag="N7A07_001382"
     CDS             2902..4731
                     /gene="recQ"
                     /locus_tag="N7A07_001382"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU2476072.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4795..5415
                     /gene="rhtC"
                     /locus_tag="N7A07_001383"
     CDS             4795..5415
                     /gene="rhtC"
                     /locus_tag="N7A07_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU2476073.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5477..6097)
                     /gene="rhtB"
                     /locus_tag="N7A07_001384"
     CDS             complement(5477..6097)
                     /gene="rhtB"
                     /locus_tag="N7A07_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU2476074.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6208..7230
                     /gene="pldB"
                     /locus_tag="N7A07_001385"
     CDS             6208..7230
                     /gene="pldB"
                     /locus_tag="N7A07_001385"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU2476075.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7238..8038
                     /gene="yigL"
                     /locus_tag="N7A07_001386"
     CDS             7238..8038
                     /gene="yigL"
                     /locus_tag="N7A07_001386"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU2476076.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8114..9013
                     /gene="bioP"
                     /locus_tag="N7A07_001387"
     CDS             8114..9013
                     /gene="bioP"
                     /locus_tag="N7A07_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU2476077.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8901..9854)
                     /gene="metR"
                     /locus_tag="N7A07_001388"
     CDS             complement(8901..9854)
                     /gene="metR"
                     /locus_tag="N7A07_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU2476078.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10090..12351
                     /gene="metE"
                     /locus_tag="N7A07_001389"
     CDS             10090..12351
                     /gene="metE"
                     /locus_tag="N7A07_001389"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU2476079.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12391..13206)
                     /gene="ysgA"
                     /locus_tag="N7A07_001390"
     CDS             complement(12391..13206)
                     /gene="ysgA"
                     /locus_tag="N7A07_001390"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU2476080.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13468..14229
                     /gene="udp"
                     /locus_tag="N7A07_001391"
     CDS             13468..14229
                     /gene="udp"
                     /locus_tag="N7A07_001391"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU2476081.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14370..15797
                     /gene="rmuC"
                     /locus_tag="N7A07_001392"
     CDS             14370..15797
                     /gene="rmuC"
                     /locus_tag="N7A07_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU2476082.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15892..16647
                     /gene="ubiE"
                     /locus_tag="N7A07_001393"
     CDS             15892..16647
                     /gene="ubiE"
                     /locus_tag="N7A07_001393"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU2476083.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16661..17266
                     /gene="ubiJ"
                     /locus_tag="N7A07_001394"
     CDS             16661..17266
                     /gene="ubiJ"
                     /locus_tag="N7A07_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU2476084.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17263..18903
                     /gene="ubiB"
                     /locus_tag="N7A07_001395"
     CDS             17263..18903
                     /gene="ubiB"
                     /locus_tag="N7A07_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU2476085.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18982..19251
                     /gene="tatA"
                     /locus_tag="N7A07_001396"
     CDS             18982..19251
                     /gene="tatA"
                     /locus_tag="N7A07_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU2476086.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19255..19770
                     /gene="tatB"
                     /locus_tag="N7A07_001397"
     CDS             19255..19770
                     /gene="tatB"
                     /locus_tag="N7A07_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU2476087.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19773..20549
                     /gene="tatC"
                     /locus_tag="N7A07_001398"
     CDS             19773..20549
                     /gene="tatC"
                     /locus_tag="N7A07_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU2476088.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20591..21373
                     /gene="tatD"
                     /locus_tag="N7A07_001399"
     CDS             20591..21373
                     /gene="tatD"
                     /locus_tag="N7A07_001399"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU2476089.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21370..21858)
                     /gene="rfaH"
                     /locus_tag="N7A07_001400"
     CDS             complement(21370..21858)
                     /gene="rfaH"
                     /locus_tag="N7A07_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU2476090.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22025..23518
                     /gene="ubiD"
                     /locus_tag="N7A07_001401"
     CDS             22025..23518
                     /gene="ubiD"
                     /locus_tag="N7A07_001401"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU2476091.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23564..24265
                     /gene="fre"
                     /locus_tag="N7A07_001402"
     CDS             23564..24265
                     /gene="fre"
                     /locus_tag="N7A07_001402"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU2476092.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24457..25620)
                     /gene="fadA"
                     /locus_tag="N7A07_001403"
     CDS             complement(24457..25620)
                     /gene="fadA"
                     /locus_tag="N7A07_001403"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU2476093.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25630..27819)
                     /gene="fadB"
                     /locus_tag="N7A07_001404"
     CDS             complement(25630..27819)
                     /gene="fadB"
                     /locus_tag="N7A07_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU2476094.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28009..29340
                     /gene="pepQ"
                     /locus_tag="N7A07_001405"
     CDS             28009..29340
                     /gene="pepQ"
                     /locus_tag="N7A07_001405"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU2476095.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29340..29954
                     /gene="yigZ"
                     /locus_tag="N7A07_001406"
     CDS             29340..29954
                     /gene="yigZ"
                     /locus_tag="N7A07_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU2476096.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29993..31444
                     /gene="trkH"
                     /locus_tag="N7A07_001407"
     CDS             29993..31444
                     /gene="trkH"
                     /locus_tag="N7A07_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU2476097.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31456..32001
                     /gene="hemG"
                     /locus_tag="N7A07_001408"
     CDS             31456..32001
                     /gene="hemG"
                     /locus_tag="N7A07_001408"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU2476098.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7472 a         8143 c         8781 g         7750 t
ORIGIN      
        1 tggcagttgt tcttgggtaa agaaccttac agcaaaaaat ctgattttgt taaggcatgg
       61 ttattgtggt gggctattac catccttgtt ctatttattt taagcctgac tacgacatta
      121 aatttaagaa gtataggtat agggttaagt tttgggtttt gtcaaatcct tggtagatat
      181 acgatgtctc taggatggag taagtactgg ggagttttgg ggggatttcc tgtaacaaat
      241 attgttcttt ttcttatctt aatttttttc aaaaaaagat aaataagtca gtgggtaggc
      301 tctttaaacg gcctacccat gttaattctt aactttttct acgctgcgta taaatcgcat
      361 ccatcacaaa aattgccagc gccacccaaa taaaggtgaa agtcaccatc ttatcggcac
      421 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
      481 ggaaaaagcc taacgttgag agacgaaggc gcgtggcagc ggcggtaaaa cacaacagcg
      541 gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc gggttttgcc
      601 ccatatggct ggttgagctg tcggcaatag caaacaggta aatcgccgcc acgggcagca
      661 gccacatggt ttcaattaac atgccggttt gtgcttctac cgcaatcttc ttgcgcacca
      721 ggccgtagaa ggcaaaacta aatgccagcc ccagcgcgat aataggtagc gaaccaaaag
      781 tccacagctg gactaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
      841 ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt
      901 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
      961 tgccaccaat cagcacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca
     1021 gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg
     1081 accagatcac gcgatgcgtc aggatctcat cggtgggcac gtagtaaatc aacttgaagt
     1141 acgctggggc tataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
     1201 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
     1261 tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc tgttcttcat
     1321 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg ctactggtag
     1381 cagtaaaacg ctcgcctctg cctgggcgca gataatcgac gcgaagatca atggtcccca
     1441 tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
     1501 ttcctacgca caccagaccg gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca
     1561 aaatgctttg cgcccagttg cccaccatca ttggctgatt tttaaaggcc agctgtgcga
     1621 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
     1681 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
     1741 ccttacactt cattggttaa tgaaatgttg attttatgat tctttgttgt tggtttctac
     1801 tttaggaagg gataactaac ggcaatggag ttaagtatgt aaaatacccc gcagaaaaac
     1861 attcacctta tcaataattc gttacggaga acacgaccga tgcggactct gcagggctgg
     1921 ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac ggtgaaagag
     1981 gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca ggagcatgac
     2041 aatccgttca cgctctatcc ttatgacacc aactacctca tctacaccca aaccagcgat
     2101 ctgaacaaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta
     2161 aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc gaactcggtg
     2221 ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga agagtcttca
     2281 ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac cgattaccgt
     2341 tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc taacgggcgt
     2401 tcagacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc agaaaacggt
     2461 aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga taacccggat
     2521 atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg tgatgcggtg
     2581 ctcagtgcga aaggacagta caactggaac accggctacg gcggcgcgga gttaggctta
     2641 agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg ctatggcgaa
     2701 tcgctcatcg actataactt caaccagacc cgtgtcggtg tgggggttat gctaaacgat
     2761 ttgttttgat gaacggttga gtggttggca aatctggaat ccagcatcca ggattaccct
     2821 ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg cctgttttta
     2881 tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga gtccggagct
     2941 aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca ggaagaaatt
     3001 atcgacactg tgctttccgg tcgcgattgc ctggtcgtca tgcccaccgg tggcggaaaa
     3061 tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg
     3121 ctgatttcgt tgatgaaaga tcaggtggat caactgcaag ccaacggcgt ggcggcggcg
     3181 tgccttaact cgacgcaaac ccgcgaacag caacttgaag tgatgacagg ctgccgcacc
     3241 gggcaaattc gtctgcttta tatcgccccg gaacgcctga tgctggataa ctttcttgag
     3301 catctggcgc actggaatcc ggtgttatta gccgttgatg aagcgcactg tatctcccaa
     3361 tggggccacg atttccgccc ggaatatgcc gcgctcggtc agttgcgcca gcggttcccg
     3421 acgctgccgt ttatggcgct gaccgccaca gccgacgaca ccacgcgcca ggatatcgtg
     3481 cgcctgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg tccgaatatt
     3541 cgctacatgc tgatggagaa attcaaaccg ctcgatcagt tgatgcgcta cgtgcaggaa
     3601 cagcgcggta agtccggcat tatctactgc aacagccggg cgaaagtaga agacaccgct
     3661 gcgcgcctgc aaagcaaggg tattagcgcg gcggcctatc atgccgggct ggaaaataat
     3721 gtccgcgccg acgtgcagga gaaattccag cgcgatgacc tgcaaattgt ggtggcgacg
     3781 gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca ctttgatatt
     3841 ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga tggcctgcct
     3901 gcggaagcga tgttgtttta cgatccagct gatatggcgt ggctgcgccg ttgtctggaa
     3961 gagaagccgc aggggcagtt gcaggatatc gagcgccaca aactcaatgc gatgggcgcg
     4021 tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga actattttgg cgaagggcgt
     4081 caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta cgacggttca
     4141 accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcggtt tgggatgggt
     4201 tatgtggtgg aagtgattcg tggtgctaat aaccagcgta tccgcgacta tggtcatgac
     4261 aaactgaaag tctatggcat gggccgtgat aaaagccatg aacattgggt gagcgtgatc
     4321 cgccagctga ttcacctcgg cctggtgacg caaaatattg cccagcattc tgccctacaa
     4381 ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct ctttacaact tgccgtgccg
     4441 cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa ctatgatcgc
     4501 aaactgttcg ccaaattacg caaactgcgt aaatcgattg ccgatgaaag taatgtcccg
     4561 ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat gccgatcacc
     4621 gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg ctttggtaaa
     4681 ccgtttatgg cgctgattcg tgcgcatgtc gatggcgacg acgaagagta gtcagcagca
     4741 taaaaaagtg ccaggatgaa gactccgtaa acgtttcccc cgcgagtcaa atgtatgttg
     4801 atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagtcc cggtcccgat
     4861 ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg
     4921 ctgggtatta cctgcggcgt tatggtttgg gcagggattg cgttgcttgg cctgcatttg
     4981 attatcgaaa aaatggcctg gctgcatacg ctgattatgg ttggcggtgg cctgtatctc
     5041 tgctggatgg gttaccagat gctgcgcggc gcgctgaaaa aagaggcggt ttctgcacct
     5101 gcgccacagg tcgagctggc gaaaagtggg cgcagtttcc tgaaaggttt actgaccaat
     5161 ctcgctaatc cgaaagcgat tatctacttt ggctcggtgt tctcattgtt tgtcggtgat
     5221 aacgttggca ctaccgcgcg ctggggcatt ttcgcgttga ttattgtcga aacgctggcg
     5281 tggtttactg tggtggccag cctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt
     5341 ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg cattcatttg
     5401 attatttcgc ggtaatgcca gacgcgtctt cagagtaagt cggataaggc gtttacgccg
     5461 catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc accagcataa
     5521 acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta atccatagag
     5581 caatccgttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc acaatagtgg
     5641 tgacaccgag cacgatatac tgcatcagtt gcggctgttg cggcatgatg aattgcggaa
     5701 atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa actgcgcgct
     5761 ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aagatcaatt gcgccagcgg
     5821 cgcgccattg ctgtattccc agccaaatca agtaagccgc gcctgcccac ttcaacactt
     5881 caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc agcacaatat
     5941 gaatcgccag tccggtctga agcccggcaa tagacgccac cgcgccgcga taaccgtggt
     6001 tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt aaaatgattg
     6061 atgtcagtag gtaggcaaac caccattcta aggtcatgat gaactcccgg tgtgtctatt
     6121 tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgctaaaa aaaacgattt
     6181 tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg ggaaacaaga
     6241 gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg tcagcgtgat
     6301 gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag
     6361 caccatgacc gggtggtagt catctgcccg gggcgtattg agagttacgt aaaatatgcg
     6421 gaactggcct acgatttgtt ccatttgggg tttgatgtct taatcatcga ccatcgcggg
     6481 cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa tcgctttaat
     6541 gattatgttg atgatctggc ggcattctgg caacaggagg tccagcccgg tccgtggcgt
     6601 aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt tctgcaacgc
     6661 catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat cgtgattcgt
     6721 atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc acgtttccgt
     6781 gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg
     6841 acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga cccaacgatt
     6901 cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg cgaacaggtg
     6961 ttagccggtg cgggtgatga cgccacgcca acgcttctct tgcaggctga agaggaacgc
     7021 gtggtggata accgcattca tgaccgtttt tgtgaactcc gcaccgccgc gggccatcct
     7081 gtcgaagggg gacgaccgct ggtaattaaa ggtgcgtacc atgagatcct ttttgaaaag
     7141 gacgcaatgc gctcagttgc gctccacgcc atcgttgatt ttttcaacag gcataactca
     7201 cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg ttgcgtctga
     7261 tttagatggc acgttacttt ctcccgacca tacattatcc ccttacgcca aagaaaccct
     7321 gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc accacgttga
     7381 tgtggggcaa attcgcgata atctggagat taagtcttac atgattacct ccaatggtgc
     7441 gcgcgttcac gatctggatg gtaatctgat ttttgctcat aacctggatc gcgacattgc
     7501 cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg tttatcgcga
     7561 cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta aagaagcggt
     7621 gttcaaatat gcgctgtatg agcctggatt actggagccg gaaggcgtca gcaaagtgtt
     7681 cttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta acgctcgttg
     7741 gggcgatcgc gtcaacgtca gtttctctac cttaacctgt ctggaagtga tggcgggcgg
     7801 cgtttcaaaa ggccatgcgc tggaagcggt ggcgaagaaa ctgggctaca gcctgaagga
     7861 ttgtattgcg tttggtgacg ggatgaacga tgccgaaatg ctgtcgatgg cggggaaagg
     7921 ctgcattatg ggcagtgcgc accagcgtct gaaagacctt catcccgagc tggaagtgat
     7981 tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt tatcgtaatc
     8041 gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact cgttaatcaa
     8101 agagttttcc attgtggcgc tacttatcat caccacgatt ctgtgggcct tctcctttag
     8161 cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt
     8221 tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc cgtggcaata gcctgaaaac
     8281 agtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata tgctgagttt
     8341 ccgcgcttat ctctacctga cggtttccga actgctgctg ttcaccgtac tgacgccgct
     8401 ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg gatatgcttt
     8461 tagtgccttg ttggcggtga ttggtgccgg gattattcgc tatgatcagg tcaccgacca
     8521 tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca tcggcatggt
     8581 gggttacaaa cgcctgatgg aaactcgccc gatgccgcag cataacgcct ttgcgtggtt
     8641 ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa atgcgcagaa
     8701 aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg tggcttccgg
     8761 gattggctac tttatgtgga actacggcgc aacccaagta gacgccggaa cgctgggcat
     8821 tatgaataat atgcacgttc cggcagggct gctggttaac ctggctatct ggcaccaaca
     8881 gccgcactgg ccaacgttta ttacaggcgc gctggtgatc ctggcctcac tgtgggtgca
     8941 tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc gtgattgcgc
     9001 gctgagcgaa taaacgcttc cgtaactggc tgacgctgct cgccatcgcg cacggcggcg
     9061 tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc ctggcgctca
     9121 aaactctcta ctacccaatg tggtagcgcg gcaataccca tccgcgcggc aaccatctga
     9181 atcaacaata aggtgttatc gacgcttttc agcgacgggc taacgcctgc gggctggaga
     9241 aaatgccgcc agacatccag tcggctacgc tgcaccggat aaatcaacag cgtttcgctg
     9301 gcgagatctt ccggcgtgat tcgcgttttc gccgccagtg gatggtcagg tgctaacacc
     9361 agacgcactt catagtcgaa catcggcgaa taatgcaggc cactacgcgg cagaatatcg
     9421 gaggtcatca ccagatccag ctcgccctgc tgcaacgcag gctgcgggtc aaacgttacg
     9481 cccgatttaa aatccatctc cacctgcggc cagttcttat ggaaattttc taatgcgggc
     9541 gtcagccact gaatacagct atggcactca atggcaatgc gcaggcgcgt ctgttgcggt
     9601 tcattgcagg cttgcagcgc ctggctaatt tgcggcagca cctggtttgc cagttgcaac
     9661 aggatttccc cctgtggagt aaagcgtagc ggctggctct tacgcacaaa tagccggaag
     9721 ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt ttgatgcaac
     9781 gtcgccgcag cggctgcgag agagccgcag ttccgcaacg cttgtagcgt tttcaggtgt
     9841 tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt gaggaatata
     9901 cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaaatcctt cgtcttttaa
     9961 atttatggtg cgttggctgc gtttctccac cccggtcact tacttcagta agctcccggg
    10021 gatgaataaa cttgccgcct tccctaaatt caaaatccat aggatttaca tataattaga
    10081 ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc
    10141 gagctgaaaa aagcgcaaga gagttattgg gcggggaact ccacgcgtga agaactgctg
    10201 gcggtagggc gtgaattgcg tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac
    10261 ctgctgccgg tgggcgattt tgcctggtac gatcatgtac tgaccaccag tctgctgctg
    10321 ggtaacgttc cggcgcgtca tcagaacaaa gatggttcgg tagatatcga caccctgttc
    10381 cgtattggtc gtggacgtgc gccgactggc gaacctgcgg cggcagcgga aatgaccaaa
    10441 tggtttaaca ccaactatca ctacatggtg ccggagttcg ttaaaggcca acagttcaaa
    10501 ctgacctgga cgcagctgct ggacgaagtg gacgaggcgc tggcgctggg ccacaaggtg
    10561 aaacctgtgc tgctggggcc gattacctgg ctgtggctgg ggaaagtgaa aggtgaacag
    10621 tttgaccgcc tgagcctgct gaacgacatt ctgccggttt atcagcaagt gctggcagaa
    10681 ctggcgaaac gtggcatcga gtgggtacag attgatgaac ccgcgctggt actggaacta
    10741 ccacaggcgt ggctggacgc atacaaaccc gcttacgacg cgctccaggg acaggtgaaa
    10801 ctgctgctga ccacctattt tgaaggcgta acgccaaatc tcgacacgat tactgcgctg
    10861 cctgttcagg ggctgcatgt ggaccttgta catggtaaag atgacgttgt tgaactgcac
    10921 aagcgcctgc cttctgactg gctgctgtct gcgggtctga tcaatggtcg taacgtctgg
    10981 cgcgccgatc ttactgagaa atatgtgcaa attaaggaca ttgtcggcaa acgtgatttg
    11041 tgggtggcat cttcctgctc gttgctgcac agccccatcg acctgagcgt ggaaacgcgt
    11101 cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac aaaaatgcca tgaactggca
    11161 ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc tggcagagtg gagcgcccca
    11221 attcaggcgc gtcgtcactc tactcgtgta cataatccgg cggtagaaaa gcgtctggcg
    11281 gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg aagtgcgtgc tgaagcccag
    11341 cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga ttggttcctt cccgcaaacc
    11401 acggaaattc gtaccctgcg tctggatttc aaaaagggca atctcgacgc caataactac
    11461 cgcacgggca ttgcggaaca tatcaggcag gccattgttg aacaggaacg tttgggactg
    11521 gatgtgctgg tacatggcga ggccgagcgt aatgacatgg tggaatactt tggcgagcac
    11581 ctcgacgggt ttgtctttac gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg
    11641 aagccaccga ttgttattgg tgacgttagt cgcccggcac cgattaccgt ggagtgggcg
    11701 aagtatgcgc aatctatgac tgataaaccg gtgaaaggga tgctgactgg cccggtgacc
    11761 attctctgct ggtcgttccc gcgtgaagat gtcagccgtg aaaccatcgc caaacaaatt
    11821 gcgctggcgc tgcgtgatga agtggcggac ctggaagccg caggaattgg catcatccag
    11881 attgatgaac cggcgctgcg cgaaggttta ccgctgcgtc gtagcgactg ggatgcatat
    11941 ctccagtggg gcgtggaggc cttccgtatc aacgccgccg tggcgaaaga tgacacacaa
    12001 atccacactc acatgtgtta ttgcgagttc aacgacatca tggattcgat tgcggcactg
    12061 gacgcagacg tcatcaccat cgaaacctcg cgttctgaca tggagttgct ggagtcgttc
    12121 gaagagtttg attatccgaa tgaaatcggt ccaggcgtct atgacattca ctcgccaaac
    12181 gtaccgagcg tggaatggat tgaagccttg ctgaagaaag cggcaaaacg cattccggca
    12241 gagcgcctgt gggtcaaccc ggactgtggc ctgaaaacgc gcggctggcc agaaacccgc
    12301 gcggcactgg cgaacatggt gcaggcggcg cagaacttgc gtcgggggta aaatccaaac
    12361 cgggtggtaa taccacccgg tcttttctca ttacagcgac ttcttcccac catactgctt
    12421 aaaccattcc agcatacgct gccagccatc ttctgcagat gcggcatgat agctcgggcg
    12481 ataatcagcg ttgaatgcat gcccggcgtc cgggtacacg ataatctctg ctttcgcatt
    12541 agcagcccgc agcgcctggc gcatggtttc aacgctctcc tgcggaatac tgttatccag
    12601 accgccatat aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt
    12661 cggtgaattc agcgacttat cgcccgtcag tttgccgtac cacgccactg cggcttttag
    12721 ctgtggatta tgcgcggcat acagccaagt gatacgtcca ccccagcaga atccggtgat
    12781 cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc
    12841 ggccagcacc tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc
    12901 ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag ccagataccc
    12961 ctccagcgcc agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg
    13021 cactacaatg accactggca gtgggccatc gctttgcttt ggtctggcat ggtaagcagg
    13081 catgttatcc ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggggt
    13141 ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca aatccagatt gttgtgttgt
    13201 tgccatggta ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat
    13261 gcaacaaatc acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt
    13321 gatttatgtg atttgcatca cctttggtgg gtaaatttat gcaacgcatt tgcgtcatgg
    13381 tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga
    13441 ctaaaccgat tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc
    13501 actaaaaacg atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg
    13561 gaaaagatcg ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcact
    13621 acctggcgtg cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc
    13681 ccgtctacct ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt
    13741 atcggtacaa cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg
    13801 gcgtctgtcc gtctggatgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc
    13861 gctgatttcg aatgtacgac tgcgctggtt gaagctgcga aatccattgg cgcgacaact
    13921 cacgttggcg tgacagcttc ttctgatacc ttctacccag gtcaggaacg ttacgatact
    13981 tactctggtc gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc
    14041 gtaatgaact atgaaatgga atctgcaacc ctgctgacca tgtgtgcaag tcagggcctg
    14101 cgtgccggta tggtagcggg tgttatcgtt aaccgcaccc agcaagagat cccgaatgct
    14161 gagacgatga aacaaactga aagccaagcg gtgaaaatcg tggtggaagc ggcgcgtcgt
    14221 ctgctgtaat tctcttctcc tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg
    14281 tagcgcctcg caggaaatgc ctttccaact ggacgtttgt acagcacaat tctattttgt
    14341 gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc aatcatggtt tacgcagtta
    14401 ttgcgttggt gggtgtggca attggctggc tgtttgccag ttatcaacat gcgcagcaaa
    14461 aagccgagca attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac
    14521 aaattaccca aagcgagcac tggcgtgcag agtgcgagtt actcaataac gaagtgcgca
    14581 gcctgcaaag tattaacacc tctctggagg ccgatctgcg tgaagtaacc acgcggatgg
    14641 aagccgcaca gcaacatgct gacgataaaa ttcgccagat gattaacagc gagcagcgcc
    14701 tcagtgagca gtttgaaaac ctcgccaacc gtatttttga gcacagcaat cgccgggttg
    14761 atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg
    14821 gtttccgccg tcaggttcag gacagcttcg gtaaagaagc acaagaacgc cataccctga
    14881 cccacgaaat tcgcaatctc cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc
    14941 tgacgcgcgc gctgaaaggc gacaataaaa cccagggcaa ctggggcgag gtagtattga
    15001 cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc caggtcagca
    15061 tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat cgtgcggctg ccgcagggaa
    15121 aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat tttaacgccg
    15181 aagacgacta cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg cgtaaccata
    15241 tccgtttgct gggacgcaaa gattatcaac agctgccggg gctgcgaact ctggattacg
    15301 tgctgatgtt tattcccgtt gaacccgctt ttttactggc gcttgaccgc cagccggagc
    15361 tgatcaccga agcgttgaaa aacaatatca tgctggttag cccgactacg ctgctggtgg
    15421 cgctgcgcac tatcgccaac ctgtggcgtt atgagcatca aagccgcaac gcccagcaaa
    15481 tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt gttcatcgat gacatgtccg
    15541 cgattggtca aagtctcgac aaagcgcagg ataactatcg gcaggcaatg aaaaaactct
    15601 cttcagggcg cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa
    15661 ttaaacgcga gattaatccg gatttggctg aacaggcggt gagccaggat gaagagtatc
    15721 gacttcggtc ggttccggag cagccgaatg atgaagctta tcaacgcgat gatgaatata
    15781 atcagcagtc gcgctagccc attgggagta gttaagccgg gtagaaatct agggcatcga
    15841 cgcccaatct gttacacttc tggaacaatt ttttgatgag caggcattga gatggtggat
    15901 aagtcacaag aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat
    15961 atggtcgccc acgttttcca ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg
    16021 tcatttggta ttcatcgttt gtggaagcga ttcacgattg attgcagcgg cgtacgccgt
    16081 gggcagaccg tgctggatct ggctggtggc accggcgacc tgacagcgaa attctcccgc
    16141 ctggtcggag aaactggcaa agtggtcctt gctgatatca atgaatccat gctcaaaatg
    16201 ggccgcgaga agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac
    16261 gctgaggcgc tgccgttccc ggataacacc tttgattgca tcaccatttc gtttggtctg
    16321 cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct gaaacccggc
    16381 ggccgcctgc tggtgcttga gttctcgaag ccaattatcg agccgctgag caaagcctat
    16441 gatgcatact ccttccatgt gctgccgcgt attggctcac tggtcgcgaa cgacgccgac
    16501 agctaccgtt atctggcaga atccatccgt atgcatcccg atcaggatac cctgaaagcc
    16561 atgatgcagg atgccggatt cgaaagtgtc gactactaca atctgacggc aggggttgtg
    16621 gcgctgcatc gtggttataa gttctgacag gagaccggaa atgcctttta aacctttagt
    16681 gacggcagga attgaaagtc tgctcaacac cttcctgtat cgctcacccg cgctgaaaac
    16741 ggcccgctcg cgtctgttgg gcaaagtgct gcgcgtggag gtaaaaggct tttcgacgtc
    16801 attgattctg gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg caggcgatgc
    16861 tgactgcacc gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc gccagcagct
    16921 tgccgccctg attcgcagtg gtgagctgga agtgcagggc gatattcagg tggtgcaaaa
    16981 cttcgttgcg ctggcaggtc tggcagagtt cgaccctgcg gaactgctgg ccccttatac
    17041 cggtgatatc gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca
    17101 tcacggcatt aagcgccagc aacgttatgt ggcggaagcc attactgaag agtggcgtat
    17161 ggcacccggt ccgcttgaag tggcctggtt tgcggaagag acggctgccg tcgagcgtgc
    17221 tgttgatgcc ctgatcaaac ggctggaaaa actggaggct aaatgacgcc aggtgaagta
    17281 cggcgcctat atttcatcat tcgcactttt ttaagctacg gacttgatga actgatcccc
    17341 aaaatgcgta tcaccctgcc gctacggcta tggcgatact cattattctg gatgccaaat
    17401 cggcataaag acaaactttt aggtgagcga ctacgactgg ccctgcaaga actggggccg
    17461 gtatggatca agttcgggca aatgttatca acccgccgcg atctttttcc gccgcatatt
    17521 gccgatcagc tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag
    17581 cagcagattg aagctgcaat gggcggcttg ccggtagaag cgtggtttga cgattttgaa
    17641 atcaagccac tggcttctgc ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat
    17701 ggtaaagagg tggtgattaa agtcatccgc ccggatattt tgccggtcat taaagcagac
    17761 ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt
    17821 ctacgcccaa ccgaagtggt gcgcgagtac gaaaagacct tgattgatga actgaatttg
    17881 ctgcgggaat ctgccaacgc cattcagctt cggcgaaatt ttgaagacag cccgatgctc
    17941 tacatcccgg aggtttaccc tgactattgt agtgaaggga tgatggtgat ggagcgtatt
    18001 tacggcattc cggtgtctga tgttgcggcg ctggagaaaa acggcaccaa catgaaattg
    18061 ctggcggaac gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag ctttttccat
    18121 gccgatatgc accctggcaa catcttcgta agctatgaac acccggaaaa cccgaaatat
    18181 atcggcattg attgcgggat tgttggctcg ctaaacaaag aagataaacg ctatctggca
    18241 gaaaacttta ttgccttctt taatcgcgac tatcgcaaag tggcagagct acacgtcgat
    18301 tctggctggg tgccaccaga taccaacgtt gaagagttcg aatttgctat tcgtacggtc
    18361 tgtgaaccta tctttgagaa accgctggcc gaaatttcgt ttggacatgt actgttaaat
    18421 ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt gttactccag
    18481 aaaaccctgc tctacgtcga aggggtagga cgccagcttt atccgcaact cgatttatgg
    18541 aaaacggcga agcctttcct ggagtcgtgg attaaagatc aggtcggtat tcctgcgctg
    18601 gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa
    18661 ttggtttacg acagtttgcg ccagggcaag tatttacagc acagtgttga taagattgcc
    18721 cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc gttattttct cggaattggc
    18781 gctacgttag tattaagtgg cacattcttg ttggtcagcc gacctgaatg ggggctgatg
    18841 cccggctggt taatggcagg tggtctgatc gcctggtttg tcggttggcg caaaacacgc
    18901 tgattttttc atcgctcaag gcgggccgtg taacgtataa tgcggctttg tttaatcatc
    18961 atctaccaca gaggaacatg tatgggtggt atcagtattt ggcagttatt gattattgcc
    19021 gtcatcgttg tactgctttt tggcaccaaa aagctcggct ccatcggttc cgatcttggt
    19081 gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg aaccaaagca ggataaaacc
    19141 agtcaggatg ctgattttac tgcgaaaact atcgccgata agcaggcgga tacgaatcag
    19201 gaacaggcta aaacagaaga cgcgaagcgc cacgataaag agcaggtgta atccgtgttt
    19261 gatatcggtt ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt tctggggccg
    19321 caacgactgc ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg
    19381 gcgacaacgg tgcagaacga actgacccag gagttaaaac tccaggagtt tcaggacagt
    19441 ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa agcgtcgatg
    19501 gatgaactac gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa
    19561 aaggcgagcg atgaagcgca caccatccat aacccggtgg tgaaagataa tgaagctgcg
    19621 catgagggtg taacgcctgc cgctgcacaa acgcaggcca gttcgccgga acagaagcca
    19681 gaaaccacgc cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca
    19741 ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt agaagatact caaccgctta
    19801 tcacgcatct gattgagctg cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga
    19861 tattcctgtg tctggtctat ttcgccaatg acatctatca cctggtatcc gcgccattga
    19921 tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg ccgttcttta
    19981 cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc agcgccggtg attctctatc
    20041 aggtgtgggc atttatcgcc ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc
    20101 tgctggtttc cagctctctg ctattttata tcggcatggc gttcgcctac tttgtggtct
    20161 ttccgctggc atttggcttc cttgccaata ccgcgccgga aggggtgcag gtatccaccg
    20221 acatcgccag ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg
    20281 aagtgccggt ggcaattgtg ctgctgtgct ggatggggat tacctcgcca gaagacttac
    20341 gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt cgggatgttg ctgacgccgc
    20401 cggatgtctt ctcgcaaacg ctgttggcga tcccgatgta ctgtctgttt gaaatcggtg
    20461 tcttcttctc acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg
    20521 aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat
    20581 ggagtacagg atgtttgata tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg
    20641 tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat gggctactca tcaccggcac
    20701 taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc
    20761 aacggcgggc gtacatcctc acgacagcag ccagtggcaa gctgcgactg aagaagcgat
    20821 tattgagctg gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc tcgactttaa
    20881 ccgcaacttt tcgacgccgg aagagcagga acgcgctttt gttgcccagc tacgcattgc
    20941 cgcagattta aacatgccgg tatttatgca ctgtcgcgat gcccacgagc ggtttatgac
    21001 attactggag ccgtggctgg ataaactgcc tggtgcggtt cttcattgct ttaccggcac
    21061 acgcgaagag atgcaggcgt gcgtggcgca tggaatttat atcggcatta ccggttgggt
    21121 ttgcgatgaa cgacgcggac tggagctgcg ggaacttttg ccgttgattc cggcggaaaa
    21181 attactgatc gaaactgatg cgccgtatct gctccctcgc gatctcacgc caaagccatc
    21241 atcccggcgc aacgagccag cccatctgcc ccatattttg caacgtattg cgcactggcg
    21301 tggagaagat gccgcatggc tggctgccac cacggatgct aatgtcaaaa cactgtttgg
    21361 gattgcgttt tagagtttgc ggaactcggt gttcttcaca ctgtgcttaa tctctttatt
    21421 aataagatta agcaatagca tggagcgagc ctcaccatcg ggttcggtga aaatggcctg
    21481 aaagccttcg aacgcgcctt cggtaataat caccttatca cccggataag gggttgccgg
    21541 atcgacaatg tctttcggtt tatataccga tagctgatga atcaccgccg atgggactat
    21601 cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg cgggtcgcgt tgatagtcgt
    21661 ggtatgaatc acttctggat caaattccac aaacaggtag ttggggaaca atggctcact
    21721 gactgcagta cgtttgccac gcacgatttt ttccagggtg atcatcggtg ccaggcaatt
    21781 cacagcctgt ctttcgaggt gttcctgggc acgttgaagt tgcccgcgct tgcagtacag
    21841 taaataccag gattgcataa tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa
    21901 gctttacgct aagttaatta tattccccgg tttgcgttat accgtcagag ttcacgctaa
    21961 tttaacaaat ttacagcatc gcaaagatga acgccgtata atgggcgcag attaagaggc
    22021 tacaatggac gccatgaaat ataacgattt acgcgacttc ttgacgttgc ttgaacagca
    22081 gggtgagcta aaacgtatca cgctcccggt ggacccgcat ctggaaatca ctgaaattgc
    22141 tgaccgcacg ctgcgtgctg gtgggcctgc gctgttgttt gaaaacccta aagggtactc
    22201 aatgccggtg ctgtgcaact tgttcggtac gccaaagcgc gtagcgatgg gtatgggcca
    22261 ggaagatgtt tcagcactgc gtgaagtcgg taaattatta gcatttctga aagaaccaga
    22321 gccgccaaaa ggttttcgcg atctgtttga taagctgccg cagtttaagc aggtgttaaa
    22381 catgccgaca aagcgactgc gcggtgcacc ctgccaacaa aaaatcgtct ctggcgatga
    22441 cgtcgatctc aaccgtattc ccattatgac ctgttggccg gaagatgccg cgccgctgat
    22501 tacatggggg ctaaccgtta cacgtggccc tcataaagag cgacagaatc tgggcattta
    22561 tcgccagcaa ctgattggta aaaacaagct gattatgcgt tggctgtcgc atcgcggcgg
    22621 cgcgctggat tatcaggagt ggtgtgcggc gcatccaggt gaacgtttcc cgatctctgt
    22681 ggcgttgggc gctgatccgg caaccattct cggtgcagtc acaccagtac cagatacttt
    22741 gtcggaatac gcctttgccg gattgctacg tggcaccaaa accgaagtag tgaagtgtat
    22801 ttccaatgat ctcgaagtgc ccgccagtgc ggagattgtg ctggaagggt atatcgaaca
    22861 aggcgaaatg gcgccagaag gaccgtatgg tgaccacact ggttactata acgaagtcga
    22921 tagtttcccg gtatttaccg tgacgcatat tacccagcgt gaagatgcga tttaccattc
    22981 cacctatacc gggcgtccgc cagatgaacc cgcggtactg ggagtggcgt tgaacgaagt
    23041 atttgttccc attctgcaaa agcagttccc ggaaattgtc gatttttacc tgccgccgga
    23101 aggctgctct tatcgcctgg cggtagtgac aatcaaaaaa cagtacgccg gacacgcgaa
    23161 gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt atgtacacta aatttgtgat
    23221 cgtttgcgat gatgacgtta acgcacgcga ctggaacgat gtgatttggg cgattaccac
    23281 ccgtatggac ccagcgcggg atactgttct ggtagaaaat acgcctattg attatctgga
    23341 ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg ctggatgcca cgaataaatg
    23401 gccgggggaa acccagcgtg aatggggacg tcccatcaaa aaagatccag atgttgtcgc
    23461 acatattgac gccatctggg atgaactggc tatttttaac aacggtaaaa gcgcctgatg
    23521 cgcgtttgtt ttgccctatt tatcgatccg acagagaaag cgcatgacaa ccttaagctg
    23581 taaagtgacc tcggtagaag ctatcacgga taccgtatat cgtgtccgca tcgtgccaga
    23641 cgcggccttt tcttttcgtg ctggtcagta tttgatggta gtgatggatg agcgcgacaa
    23701 acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg
    23761 cgcttctgaa atcaaccttt acgcgaaagc agtcatggac cgcatcctca aagatcatca
    23821 aatcgtggtc gatattcccc acggagaagc gtggctgcgc gatgatgaag agcgtccgat
    23881 gattttgatt gcgggcggca ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt
    23941 ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc gggcgtgaag agcagcatct
    24001 gtatgatctc tgcgagcttg aggcgctttc gttgaagcat cctggtctgc aagtggtgcc
    24061 ggtggttgaa caaccggaag cgggctggcg tgggcgtact ggcaccgtgt taacggcggt
    24121 attgcaggat cacggtacgc tggcagagca tgatatctat attgccggac gttttgagat
    24181 ggcgaaaatt gcccgtgacc tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt
    24241 tggcgatgcg tttgcattta tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga
    24301 acggcaacta aaactttatt cagtggcatt tagatctatg tcgcatccgg cattcggcac
    24361 acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgcc ggatcggtag
    24421 accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa acaccgtcgc
    24481 aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt ctttgtgttc
    24541 catcagattc agcagcgtgg tgctgatacg cgcaccggaa caacccagcg gatgacccag
    24601 cgcgatcgcg ccaccgttga ggttgatctt ctcgtcaatc tgctcaatta gtcccagatc
    24661 tttaatacat ggcaggatct gcgcggcaaa ggcttcattc atttcaaaca cgccgatatc
    24721 actgacagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc
    24781 catgatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc gcggcttaag
    24841 acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag
    24901 tgcagaagat gtgcccgccg ttaccgtacc gtttactgga tcaaacgccg gacgcagcgt
    24961 ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcag
    25021 gacgccgtcg gcatcgtgac caccagttgg gatgatttca tttttaaatg cgcccgactg
    25081 cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct
    25141 gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc
    25201 gacattgcgg ctcaggccag ggtgaaaatc gacgccgtga ctcatcggca catggcccat
    25261 atgctccacg ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc
    25321 gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acagtaaccg ccgggacaga
    25381 gtgcggcact tctgccagca gcgccgcgtt acgggcgata ttaaaaccct gctccagcgt
    25441 ctgctgcaca cacccccagt aaatatcgtc gagggccgcc gcttccagcg ccgggttacg
    25501 cgccagcagg ctacgcatta aatgagcgga gagatcttct gcacgcacgt tacgaaaagc
    25561 accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat
    25621 tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata
    25681 gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcgt acagcgggcc
    25741 gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctgccgaggg tgtccagcca
    25801 gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc
    25861 ttctgccgga gtggcgataa tgccttcttc cagacagcgc accacttcgt tgaccatcgg
    25921 gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct ggctcacttc
    25981 tgccagcagg tcgtcaacag cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc
    26041 tttataatgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat caaacagcgc
    26101 gtcgatggca tcgcgataat ctttctgcat ccgctgtggg aagcccgctg ccatgactgc
    26161 ctgggcgtga tgcgcggtgt caatgcccac aacgtccagc agatatgccg gacccatagg
    26221 ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag
    26281 caactggctg aaaccggcga aatacgggaa cagcacgcgg ttgacgaaga agccggggca
    26341 atcgttaacc acaatcggcg ttttacccat cttgctcgcc caggcgacga ctttcgcaat
    26401 agtttcgtcg gagcttttct cgccgcgaat aatttccacc aacggcattc ggtggaccgg
    26461 gttaaagaag tgcatcccgc agaagttttc cggacgttcc agcgcgttgg ccagttcgct
    26521 gataggaatg gttgaagtgt tagacgccag cacggtatcc gggcgtactt tctgctcggt
    26581 ttctgccagc acggcttttt tcactttcgg gttttcaaca atcgcttcta ccacaacatc
    26641 cacgcgctca aatccggcgt agtcgagcgt tgggtggatc gtggagatca cgccagccag
    26701 tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agtagtttcg cggcttcagt
    26761 catgccgagg gctaacgact tgtcgttaat atctttcata atcaccggca cgcctttcca
    26821 cgcagactgg taagcgatgc cgccacccat aatgcctgcg cccagcacgg cggcctgttt
    26881 cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag
    26941 gaaaatgccg accagtgcgc gagcttcgtt ggtatgcgcc agcgggacaa agcttttgtt
    27001 ttctaggttt aaggcttctt cacgaccaaa acgagccgca gcttcaatgg tttttactgc
    27061 ggtgatgggg gccgggtaat gttttcctgc cgtttgtgcg accatccctt tagcgatggt
    27121 gaagctcatg gtggcttcaa tcttgctcag ttttagcggt tccagcttcg gctgacgttt
    27181 cgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa atcgccatcg ccccttcaat
    27241 cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc
    27301 gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca tacgtggcat
    27361 acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg
    27421 cagatccggc gtcgccagac ggtaatcggt cgccagcacg cattcgcagc caccgcccag
    27481 cgcatagcca ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac
    27541 gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa
    27601 aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc
    27661 ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc
    27721 agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc
    27781 cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa
    27841 ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa tatgcaaata
    27901 aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga
    27961 tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg
    28021 gcctcgctct ataaaaatca tatagctaca ttgcaggaac gaactcgcga tgcgctgacg
    28081 cgcttcaagc tggatgcgtt acttattcac tccggcgaac tgttcaacgt ttttctcgac
    28141 gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag
    28201 gtgccaaact gctggctgct ggtggatggc gtgaataagc cgaaactgtg gttctatctg
    28261 ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac tgaagatgta
    28321 gaagtgatcg cgctgccgaa agccgatggc attggtagtc tgttgcctgc tgcgcgcggc
    28381 aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc aactgggtat tgaggccagc
    28441 aatatcaacc cgaaaggggt tatcgactac ctgcattact accgctcctt caaaaccgag
    28501 tacgagctgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgcgcggca
    28561 gaagaagcgt tccgttctgg catgagcgag ttcgatatca atattgccta tctgactgcg
    28621 accggtcatc gtgataccga cgtaccttac agcaacattg tggcactcaa cgaacacgct
    28681 gcggtgctgc attacaccaa actggatcat caggcgtcgg aagagatgcg cagcttcctg
    28741 ctggatgccg gggccgaata taacggctat gccgctgacc tgacccgtac ctggtcggca
    28801 aaaagtgaca acgattacgc acagctggtg aaagacgtaa atgatgaaca actggcgctg
    28861 atcgcgacca tgaaagctgg cgttagctat gtggattacc acatccagtt ccatcagcgc
    28921 atcgccaaat tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga ggcgatggtc
    28981 gaaaacgatc ttaccgggcc gtttatgccg catggtatcg gccatccgct gggcctgcag
    29041 gtgcatgacg tcgccggttt tatgcaggat gatagcggta cgcacctcgc ggcaccggca
    29101 aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa
    29161 ccgggtatct acttcattga atcgctgctg gcaccgtggc gtgaagggca gttcagcaag
    29221 cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac
    29281 aacgtggtga tccacgaaaa taacgtggaa aacatgaccc gggatctgaa actggcgtga
    29341 tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaaaagcc
    29401 gcttcattac gctgttggcg cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat
    29461 cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc
    29521 cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcggga acggcaggta
    29581 aaccgatgct cgcccagcta atgggcagcg gcgttgggga aattaccgct gtggtggtgc
    29641 gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg
    29701 tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc gaatatactt
    29761 tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca
    29821 aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga
    29881 aagtggctga attttccgcg aagctggcgg attttagccg tggttcattg caattgttag
    29941 cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt
    30001 tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat
    30061 ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt
    30121 ttttgtcgcc ctcgccattg gctctatgct gtggtggccg aaccgcaaag agaagggcga
    30181 actgaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagtgt
    30241 cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt
    30301 tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc tggattcgct
    30361 ccctcatgcc atcctctttt atcgccagat gctgcaatgg tttggcggga tggggatcat
    30421 cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc
    30481 agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa
    30541 aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc
    30601 cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta ttggcggctt
    30661 ctcgacacat gatgccagta tcggttattt cgatagcccg actattaaca ctatcattgc
    30721 tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt taagtgggcg
    30781 tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagtttac
    30841 gctggtggtt atttgtaccc tcgtactgtg gtttcataat gtctacagtt cggcgctgat
    30901 gacaattaac caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacgac
    30961 tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat
    31021 cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt
    31081 taagcagggg aaccgtgaac tgaaacgact ggtgcatccg aacgccgtct atagcattaa
    31141 gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc
    31201 ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt
    31261 ttctgccttt gcctcggttg ttgcgacatt gaataacctg gggccagggc ttggcgtggt
    31321 tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct
    31381 gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga
    31441 atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc
    31501 gagattgcct cctacttggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc
    31561 aatgtgcacc gcattgaaga accacagtgg gaaaactacg accgtgtggt cattggtgct
    31621 tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa gcatgcgacg
    31681 cggctgaatt cgatgccgag cgcgttttac tccgtaaacc tggtggcgcg taaaccggag
    31741 aagcgcactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt
    31801 cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac
    31861 cgttttatga tcaagctgat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa
    31921 gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc
    31981 gacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcaga aaattatttt
    32041 aaatttcccc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
    32101 acggcaaaca cgccgccggg tcagcgggat tctcctgaga attccg
//
LOCUS       DBFIFS010000033        32085 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.033, whole genome shotgun sequence.
ACCESSION   DBFIFS010000033 DBFIFS010000000
VERSION     DBFIFS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.033"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(73..1833)
                     /gene="ycaO"
                     /locus_tag="N7A07_001409"
     CDS             complement(73..1833)
                     /gene="ycaO"
                     /locus_tag="N7A07_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU2476099.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1963..2655
                     /locus_tag="N7A07_001410"
     CDS             1963..2655
                     /locus_tag="N7A07_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU2476100.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2854..3942
                     /gene="serC"
                     /locus_tag="N7A07_001411"
     CDS             2854..3942
                     /gene="serC"
                     /locus_tag="N7A07_001411"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU2476101.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4013..5296
                     /gene="aroA"
                     /locus_tag="N7A07_001412"
     CDS             4013..5296
                     /gene="aroA"
                     /locus_tag="N7A07_001412"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU2476102.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5465..6229
                     /gene="ycaL"
                     /locus_tag="N7A07_001413"
     CDS             5465..6229
                     /gene="ycaL"
                     /locus_tag="N7A07_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU2476103.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6402..7085
                     /gene="cmk"
                     /locus_tag="N7A07_001414"
     CDS             6402..7085
                     /gene="cmk"
                     /locus_tag="N7A07_001414"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU2476104.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7196..8869
                     /gene="rpsA"
                     /locus_tag="N7A07_001415"
     CDS             7196..8869
                     /gene="rpsA"
                     /locus_tag="N7A07_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU2476105.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9029..9313
                     /gene="ihfB"
                     /locus_tag="N7A07_001416"
     CDS             9029..9313
                     /gene="ihfB"
                     /locus_tag="N7A07_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU2476106.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9520..11784
                     /locus_tag="N7A07_001417"
     CDS             9520..11784
                     /locus_tag="N7A07_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU2476107.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11821..13569
                     /gene="msbA"
                     /locus_tag="N7A07_001418"
     CDS             11821..13569
                     /gene="msbA"
                     /locus_tag="N7A07_001418"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU2476108.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13566..14552
                     /gene="lpxK"
                     /locus_tag="N7A07_001419"
     CDS             13566..14552
                     /gene="lpxK"
                     /locus_tag="N7A07_001419"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU2476109.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14589..15821
                     /gene="ycaQ"
                     /locus_tag="N7A07_001420"
     CDS             14589..15821
                     /gene="ycaQ"
                     /locus_tag="N7A07_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU2476110.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15873..16055
                     /gene="ycaR"
                     /locus_tag="N7A07_001421"
     CDS             15873..16055
                     /gene="ycaR"
                     /locus_tag="N7A07_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU2476111.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16052..16798
                     /gene="kdsB"
                     /locus_tag="N7A07_001422"
     CDS             16052..16798
                     /gene="kdsB"
                     /locus_tag="N7A07_001422"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU2476112.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16952..17845
                     /locus_tag="N7A07_001423"
     CDS             16952..17845
                     /locus_tag="N7A07_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU2476113.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17822..18601)
                     /gene="elyC"
                     /locus_tag="N7A07_001424"
     CDS             complement(17822..18601)
                     /gene="elyC"
                     /locus_tag="N7A07_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU2476114.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18737..19522
                     /gene="cmoM"
                     /locus_tag="N7A07_001425"
     CDS             18737..19522
                     /gene="cmoM"
                     /locus_tag="N7A07_001425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU2476115.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19519..20841
                     /gene="mukF"
                     /locus_tag="N7A07_001426"
     CDS             19519..20841
                     /gene="mukF"
                     /locus_tag="N7A07_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU2476116.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20822..21526
                     /gene="mukE"
                     /locus_tag="N7A07_001427"
     CDS             20822..21526
                     /gene="mukE"
                     /locus_tag="N7A07_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU2476117.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21526..25986
                     /gene="mukB"
                     /locus_tag="N7A07_001428"
     CDS             21526..25986
                     /gene="mukB"
                     /locus_tag="N7A07_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU2476118.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26247..28094
                     /gene="ldtD"
                     /locus_tag="N7A07_001429"
     CDS             26247..28094
                     /gene="ldtD"
                     /locus_tag="N7A07_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2476119.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28275..28823
                     /locus_tag="N7A07_001430"
     CDS             28275..28823
                     /locus_tag="N7A07_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU2476120.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28850..29497
                     /gene="gloC"
                     /locus_tag="N7A07_001431"
     CDS             28850..29497
                     /gene="gloC"
                     /locus_tag="N7A07_001431"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU2476121.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29548..30738)
                     /gene="aspC"
                     /locus_tag="N7A07_001432"
     CDS             complement(29548..30738)
                     /gene="aspC"
                     /locus_tag="N7A07_001432"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU2476122.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30923..32011)
                     /gene="ompF"
                     /locus_tag="N7A07_001433"
     CDS             complement(30923..32011)
                     /gene="ompF"
                     /locus_tag="N7A07_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU2476123.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7803 a         7823 c         8630 g         7829 t
ORIGIN      
        1 gggcattaaa aaaacgcccc gtaatataac tcagacgaat cattaagcct acattacgta
       61 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
      121 cttcaacagc gactgatgtg cagcaaacgc gtgcagatcg ctatctactg gttgcaagcc
      181 ataaaacgcc gcttcgccgc tcattgccgc actggcggct tccacggcat ctgcgccgta
      241 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gcgccagtaa
      301 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc gggctgaata ccgatgagtt
      361 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
      421 cagcatggct tttaattcac caatacgcag ggtgtaccaa ccgttatccg acccggtcgc
      481 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
      541 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
      601 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
      661 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
      721 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
      781 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
      841 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
      901 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
      961 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     1021 aaagcaggtg ccgttagcag gattaaacag taccacgcaa atcaccggat actggccgcc
     1081 aagggaacca tcatatgcga agatcgggaa accttctgct tccagtgttt cgatcgcttc
     1141 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     1201 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     1261 ttcgttacgg gtattgccag cagacatacc gttagaaacg tacaggttac caatgatatt
     1321 catcggaata taaacggtct gattatcaga ctgacgtgta aacggcagac cgcaaatacc
     1381 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     1441 atcgtaaaat gcgcgcagac gttcatcgag cagcccttct ggcacatcgt cattttcggt
     1501 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     1561 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     1621 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     1681 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     1741 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagtgc
     1801 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     1861 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     1921 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     1981 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     2041 ctttatacct ttgtactggt ctttttgttc ctcaaaatga ccggaagacg cggtgtgcgg
     2101 cagatgtcgc tgtttgaagt tttaatcatt ctgacgctgg gatcagcggc gggagatgtg
     2161 gcgttttatg atgatgtgcc gatggtcccg gtacttatcg tctttattac tctggcgttg
     2221 ttataccgcc tggtaatgtg gttgatggcg cacagtgaaa aactggaaga tcttctggaa
     2281 ggcaagccag ttgtcattat tgaagatggc gagctggcct ggtcgaaact caataactcc
     2341 aacatgacgg aatttgagtt ctttatggag ctacgattgc gtggcgtgga gcagctgggg
     2401 caggtacgtc tggcgattct cgaaaccaac gggcaaatca gtgtctattt ctttgaagat
     2461 gacaaggtga aatcgggttt acttatttta cccagtgatt gtacgcagcg ttacaaagtg
     2521 gtgccggagt cggcggacta tgcctgtatt cgttgtagtg aaatcattca tatgaaagcg
     2581 ggggaaaaac aattatgtcc gcgctgtgca aatccagaat ggacgaaggc aagtcgggca
     2641 aaacgggtga cctgacagta aaaacatcgg ctttttgcta ataatccgag agattctttt
     2701 gtgtgatgca agccacattt ttgccctcaa cggttttact cattgcgatg tgtgtcactg
     2761 aatgataaaa ccgatagcca caggaataat gtattacctg tggtcgcaat cgattgaccg
     2821 cgggttaata gcaacgcaac gtggtgaggg gaaatggctc aaatcttcaa ttttagttct
     2881 ggtccggcaa tgctaccggt agaggtgctt aaacaggctc aacaggaact gcgcgactgg
     2941 aacggtcttg gtacgtcggt gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt
     3001 gcagaggaag ccgagaagga ttttcgcgat cttcttaatg tcccctccaa ctacaaggta
     3061 ttattctgcc atggcggtgg tcgcggtcag tttgctgcgg taccgctgaa tattctcggt
     3121 gataaaacca ccgcagatta tgttgatgcc ggttactggg cggcaagtgc cattaaagaa
     3181 gcgaaaaaat actgcacgcc taatgtcttt gacgccaaag tgactgttga tggtctgcgc
     3241 gcggttaagc caatgcgtga atggcaactc tctgataatg ctgcttatat gcattactgc
     3301 ccgaatgaaa ccatcgacgg tatcgccatc gacgaaacgc cagacttcgg caaagatgtg
     3361 gtggtcgccg ccgacttctc ttcaaccatt ctttcccgtc cgattgacgt cagccgttat
     3421 ggtgtaattt acgctggcgc gcagaaaaat atcggcccgg caggcctgac aatcgtcatc
     3481 gtccgtgaag atttgctggg caaagcgaat gtcgcgtgtc cgtcgattct ggattattcc
     3541 atcctcaacg ataacggctc catgtttaac acgccgccga catttgcctg gtatctatct
     3601 ggtctggtct ttaaatggct gaaagcgaac ggcggtgtag ctgaaatgga taaaatcaat
     3661 cagcaaaaag cagaactgct atatggggtg attgataaca gcgatttcta ccgcaatgac
     3721 gtggcgaaag ctaaccgttc gcggatgaac gtgccgttcc agttggcgga cagtgcgctt
     3781 gacaaattgt tccttgaaga gtcttttgct gcaggccttc atgcactgaa aggtcaccgt
     3841 gtggtcggcg gaatgcgcgc gtctatttat aatgccatgc cgctggaagg cgttaaagcg
     3901 ctgacagact tcatggttga gttcgaacgc cgtcacggtt aatgccgaaa ttttgcttaa
     3961 tccccacagc cagcctgtgg ggtttttatt tctgttgtag agagttgagt tcatggaatc
     4021 cctgacgtta caacccatcg ctcgtgtcga tggcactatt aatctgcccg gttccaagag
     4081 cgtttctaac cgcgctttat tgctggcggc attagcacac ggcaaaacag tattaaccaa
     4141 tctgctggat agcgatgacg tgcgccatat gctgaatgca ttaacagcgt taggggtaag
     4201 ctatacgctt tcagccgatc gtacacgttg cgaaattatc ggtaacggcg gtccattaca
     4261 cgcagaaggt gccctggagt tgttcctcgg taacgccgga acggcaatgc gtccgctggc
     4321 ggcagctctt tgtctgggta gcaatgatat tgtgctgacc ggtgagccgc gtatgaaaga
     4381 gcgcccgatt ggtcatctgg tggatgcgct gcgcctgggc ggggcgaaga tcacttacct
     4441 ggaacaagaa aattatccgc cgttgcgttt acagggcggc tttactggcg gcaacgttga
     4501 cgttgatggc tccgtttcca gccaattcct caccgcactg ttaatgactg cgcctcttgc
     4561 gccggaagat acggtgattc gtattaaagg cgatctggtt tctaaacctt atatcgacat
     4621 cacactcaat ctgatgaaga cgtttggtgt tgaaattgaa aatcagcact atcaacaatt
     4681 tgtcgtaaaa ggcgggcagt cttatcagtc tccgggtact tatttggtcg aaggcgatgc
     4741 atcttcggct tcttactttc tggcagcagc agcaatcaaa ggcggcactg taaaagtgac
     4801 cggtattgga cgtaacagta tgcagggtga tattcgcttt gctgatgtgc tggaaaaaat
     4861 gggcgcgacc atttgctggg gcgatgatta tatttcctgc acgcgtggtg aactgaacgc
     4921 tattgatatg gatatgaacc atattcctga tgcggcgatg accattgcta cggcggcgtt
     4981 atttgcaaaa ggcaccacca cgctgcgcaa tatctataac tggcgtgtta aagaaaccga
     5041 tcgcctgttt gcgatggcaa cagaactgcg taaagtcggt gcggaagtag aagaggggca
     5101 cgattacatt cgtatcactc caccggaaaa actgaatttt gccgagatcg cgacatacaa
     5161 tgatcaccgg atggcgatgt gtttctcgct ggtggcgttg tcagatacac cagtgacgat
     5221 tcttgatccc aaatgtacgg ccaaaacatt tccggattat ttcgagcagc tggcgcggat
     5281 tagccaggca gcctgaatga ataacgtcca ataaatagcc aaaactttct ttatcaaaac
     5341 gtcggcacat tgccggcgtt ttttttcgga ccttgtgagt cattttgatt aatggtagcg
     5401 tcgcttgtca atgtaagttg ttgatacata atgtttatat atgattaatc aacggatgat
     5461 tcacatgaag aatactaaat tactgctggc gattgtgacc tctgcagcat tactgacggg
     5521 gtgtcaaaat acccacggta ttgataccaa tatggctatc agctccggtt taaatgccta
     5581 taaagcagca acgttaagcg atgccgatgc aaaagcgatt gccaatcagg gctgtgccga
     5641 aatggacagc ggcaatcaag tcgcaagtaa atccagcaag tacggtaaac gtctggcaaa
     5701 aatcgccaaa gcattgggta acaatattaa tggcacgccg gtcaactata aggtttatat
     5761 gaccagcgac gtcaacgcat gggcgatggc gaacggctgt gttcgtgttt acagtggcct
     5821 gatggacatg atgaacgata acgaaattga aggcgttctg ggccatgaac tgggccacgt
     5881 cgcgttgggt cactcgctgg ctgaaatgaa agcttcttat gcgatcgttg ccgcacgcga
     5941 tgccatttca gctaccagcg gtgtggcttc ccagctttcc cgctcacaat tgggtgatat
     6001 cgcagaaggc gctatcaatg cgaaatactc ccgcgataaa gagtccgaag cagatgattt
     6061 ctcctttgat ctgttgaaga aacgtggcat cagcacccag gggctggttg gcagctttga
     6121 aaaactggct agcctggatg gcggtcgcac ccagtccatg tttgactctc acccaccatc
     6181 aacagagcgt gcgcaacaca tccgtgatcg tatcgcctct ggtaagtaaa tcattgtcat
     6241 ctttcgggct ggtcttctgc cagcccgcta taattgcgca ataaatcccc atctgaatac
     6301 agacaaaact ggtttttgca cacaacgtta acgatttgtg gcgtcggcgc gtataatgcg
     6361 cgcggttatg ttaacggtac gcctgtttta aggagataaa gatgacggca attgccccgg
     6421 ttattaccat tgatggccca agcggtgcag ggaaaggcac cttgtgtaag gctatggcgg
     6481 aagcgttgca atggcatctg ctggattcgg gtgcaattta tcgcgtactg gcactagcgg
     6541 cattacatca ccatgttgat gttgcatcgg aagatgcgct ggtgccgctg gcatcccatc
     6601 tggatgtacg ttttgtgtca accaatggca atctggaagt gatcctcgaa ggggaagatg
     6661 tcagcggcga aattcgtact caggaagtgg cgaatgcagc ttcacaagtc gcggcattcc
     6721 cacgcgttcg tgaagcatta ttgcgtcgcc aacgcgcgtt tcgcgaatta ccaggcctga
     6781 ttgccgatgg ccgcgacatg ggaacggtgg tattccctga tgcgccagtg aaaattttcc
     6841 ttgacgcctc ctcggaagaa cgtgcgcatc gccgcatgct acagttgcag gagaagggct
     6901 ttagtgttaa ctttgagcgc cttttggccg agatcaaaga acgcgacgac cgcgatcgta
     6961 accgagcggt agcgccactg gttccggcag ccgatgcttt agtgttggat tccaccacct
     7021 taagcattga gcaagtgatt gaaaaagcgc tacaatacgc gcgccagaaa ttggctctcg
     7081 cataagcgac cgaatttgca gtacccccgt tgcaatggaa tgacagcggg tatgttaaac
     7141 aaccccatcc ggcatggagc caggtggacg ttaaatataa acctgaagat taaacatgac
     7201 tgaatctttt gctcaactct ttgaagagtc cttaaaagaa atcgaaaccc gcccgggttc
     7261 tatcgttcgt ggcgttgttg ttgctatcga caaagacgta gtactggttg acgctggtct
     7321 gaaatctgag tccgccatcc cggctgagca gttcaaaaac gcccagggcg agctggaaat
     7381 ccaggtaggt gacgaagttg acgttgctct ggacgcagta gaagacggct tcggtgaaac
     7441 tctgctgtcc cgtgagaaag ctaaacgtca cgaagcctgg atcacgctgg aaaaagctta
     7501 cgaagatgct gaaactgtta ccggtgttat caacggcaaa gttaagggcg gcttcactgt
     7561 tgagctgaac ggtattcgtg cgttcctgcc aggttctctg gtagacgttc gtccggtgcg
     7621 tgacactctg cacctggaag gcaaagagct tgaattcaaa gtaatcaagc tggatcagaa
     7681 gcgcaacaac gttgttgttt ctcgtcgtgc cgttatcgaa tccgaaaaca gcgcagagcg
     7741 cgatcagctg ctggaaaacc tgcaggaagg catggaagtt aaaggtatcg ttaagaacct
     7801 cactgactac ggtgcattcg ttgatctggg cggcgttgac ggcctgctgc acatcactga
     7861 catggcctgg aaacgcgtta agcatccgag cgaaatcgtc aacgtgggcg acgaaatcac
     7921 tgttaaagtg ctgaagttcg accgcgaacg tacccgtgta tccctgggcc tgaaacagct
     7981 gggcgaagat ccgtgggtag ctatcgctaa acgttatccg gaaggtacca aactgactgg
     8041 tcgcgtgacc aacctgaccg actacggctg cttcgttgaa atcgaagaag gcgttgaagg
     8101 cctggtacac gtttccgaaa tggattggac caacaaaaac atccacccgt ccaaagttgt
     8161 taacgttggc gatgtagtgg aagttatggt tctggatatc gacgaagaac gtcgtcgtat
     8221 ctccctgggt ctgaaacagt gcaaagctaa cccgtggcag cagttcgcgg aaacccacaa
     8281 caagggcgac cgtgttgaag gtaaaatcaa gtctatcact gacttcggta tcttcatcgg
     8341 cctggacggc ggcatcgacg gcctggttca cctgtctgac atctcctgga acgttgcagg
     8401 cgaagaagca gttcgtgaat acaaaaaagg cgacgaaatc gctgcagttg ttctgcaggt
     8461 tgacgcagaa cgtgaacgta tctccctggg cgttaaacag ctcgcagaag atccgttcaa
     8521 caactgggtt gctctgaaca agaaaggcgc tatcgtaacc ggtaaagtaa ctgcagttga
     8581 cgctaaaggc gcaaccgtag aactggctga cggcgttgaa ggttacctgc gtgcttctga
     8641 agcatcccgt gaccgcgttg aagacgctac cctggttctg agcgttggcg acgaagttga
     8701 agctaaattc accggcgttg atcgtaaaaa ccgtgcaatc agcctgtctg ttcgtgcgaa
     8761 agacgaagct gacgagaaag atgcaatcgc aactgttaac aaacaggaag atgcaaactt
     8821 ctccaacaac gcaatggctg aagctttcaa agcagctaaa ggcgagtaat tctctgactc
     8881 ttcgggattt ttattctgaa gtttgttgag tttacttgac agattgcagg tttcgtcctg
     8941 taatcaagca ctaagggcgg ctacggccgc ccttaatcaa tgcagcaaca gcagccgctt
     9001 aatttgcctt taaggaaccg gaggaatcat gaccaagtca gaattgatag aaagacttgc
     9061 cacccagcaa tcgcacattc ccgccaagac ggttgaagat gcagtaaaag agatgctgga
     9121 gcatatggcc tcgactcttg cgcagggcga gcgtattgaa atccgcggtt tcggcagttt
     9181 ctctttgcac taccgcgcac cacgtaccgg acgtaatccg aagactggcg ataaagtaga
     9241 actggaagga aaatacgttc ctcactttaa acctggtaaa gaactgcgcg atcgcgccaa
     9301 tatttacggt taagttttta ctcaaacttg aacgagagaa aagcacctgt cgggtgcttt
     9361 tttcatttat ctaatctgga actggaagct gcctcgcaga gttttgagca gttttcaccc
     9421 tttcgttaaa ttcttctgaa tgtgcctcgg ggaacgcaaa attcccacca gacaaccgct
     9481 caacaaagtt gcacactttc cataaacagg gaggggtgca tgaaaataac gacagtcggc
     9541 gtatgcataa tttgcggaat ttttccgttg ctgattttgc cccaattgcc tgggacatta
     9601 acccttgcgt ttctgactct cttcgcctgc gtactggcat ttatccctgt taaaaccgtc
     9661 cgttatatcg cgctgacgtt gctgtttttc gtttggggca tattatcagc aaagcaaatt
     9721 ttgttggcag gagaaacctt aactggcgcg acgcaggatg caattgttga gatcactgct
     9781 actgacggca tgaccactca ttacggtcaa attacttatc tacaaggtca acgtatattc
     9841 cctgcgccag gccttgtgct gtatggcgaa tatcttccgc aagcggtttg tgccggacaa
     9901 ctgtggtcaa tgaaactcaa agttcgtgcc gttcatggtc aacttaatga tggcggcttt
     9961 gatagccagc gttatgccat tgcccagcat cagccgctca ccggccgctt tctgcaggca
    10021 agtgttattg aaccgaattg tagcctgcgt gcacagtatc tggcgtcact acaaacaacg
    10081 ctgcaaccct atccgtggaa tgcggttatt cttggtttag gtatggggga acggttatcc
    10141 gtccctaaag aaatcaaaaa tatcatgcgt gatactggaa cggcgcattt aatggcgata
    10201 tcgggattgc acatcgcttt tgcggcgttg ctggctgccg gactcattcg cagtggacaa
    10261 atttttctgc ctgggcgctg gatccactgg caaatgccat taattggcgg aatctgctgt
    10321 gctgcttttt atgcctggct gacgggaatg caacctcctg cattgcgtac cgttgtggcg
    10381 cttgctacgt ggggtatgct taagttaagt gggcgacagt ggagtggctg ggatgtatgg
    10441 atatgttgtc tggcggcaat tttgctgatg gatcctgttg tcattctctc gcaaagttta
    10501 tggctctctg ccgctgcggt cgcggcattg atattttggt atcagtggtt tccctgtcct
    10561 gagtggcaac tgccgccggt attgcgtgca gttgtttccc tcatccatct gcaactggga
    10621 atcacacttc tgcttatgcc cgtgcaaatc gtcatttttc atggcattag tctgacctcg
    10681 tttattgcaa atctattagc aattcccttg gtgacattta tcacggttcc gttgatcctc
    10741 gccgcgatgg ttgtgcattt aagcgggccg ttaatcctgg atcaagggtt atggtttctt
    10801 gccgaccggt ctttggcttt acttttcttg gggttaaaga gtttgccaga agggtggatc
    10861 aacattgctg aacgttggca atggctatca ttttccccat ggttcttact ggtggtatgg
    10921 cgattaaacg cctggcgaac gttgccagca atgtgtgtgg ctgtaggctt gctgatgtgc
    10981 tggccgctgt ggcaaaaacc tcgacctgac gagtggcaag tgtacatgct tgatgtcggg
    11041 caagggctgg caatggtgat agccagaaac ggcaaagcga ttctctatga cacgggactg
    11101 gcctggcctg aaggggatag tgggcaacaa ctgattatcc cctggctcca ctggcataat
    11161 cttgaaccgg aaggcgttat cctgagtcat gaacatctgg atcaccgggg agggctggac
    11221 tcaatattgc atacatggcc gatgttatgg atcagaagtc cgttaaactg ggaacatcat
    11281 cagccctgtg tgcgtggcga agcgtggcaa tggcaaggat tgcgtttcag cgcgcactgg
    11341 cctttacaag gtcgcaacga taaaggaaat aaccattcct gtgtggttaa ggttgatgac
    11401 gggacgaata gcattcttct aaccggtgat attgaagccc cagctgaaca aaagatgcta
    11461 agccgttact ggcagcaagt gcaggcaaca ttgcttcagg tacctcacca tggcagtaat
    11521 acctcatcat cattgccatt aattcagcga gtgaatggaa aagtggcact cgcatcggca
    11581 tcgcgctata acgcatggcg actgccctct aacaaagtta agcatcgcta tcaacaacaa
    11641 ggttatacgt ggcttgatac tcctcatcag gggcaagtaa cggtcgattt ttcagcgcaa
    11701 ggctggcgga ttagcagcct cagggagcaa attttacctc gttggtatca tcagtggttt
    11761 ggcgtgccag tggataacgg gtagaatatg cggctatttc aacaaatgct ggttttttga
    11821 atgcataacg acaaagatct ctctacgtgg cagacattcc gccgactgtg gccaaccatt
    11881 gcgcctttta aagcgggtct gatcgtggcg ggcgtagcgt taatcctcaa cgcagccagc
    11941 gataccttca tgttatcgct ccttaagcca cttcttgatg atggctttgg taaaacagat
    12001 cgctccgtgc tggtgtggat gccgctggtg gtgatcgggc tgatgatttt gcgtggtatc
    12061 accagctatg tctccagcta ctgtatctcc tgggtatcag gaaaggtggt aatgaccatg
    12121 cgtcgccgcc tgtttggtca catgatggga atgccggttt cattctttga caaacagtca
    12181 acgggtacgc tgttgtcacg tatcacctac gattccgaac aggttgcttc ttcctcttcc
    12241 ggcgcactga ttactgttgt gcgtgaaggt gcgtcgatca tcggcctgtt catcatgatg
    12301 ttctattaca gttggcaatt gtcgatcatt ttgattgtgc tggcaccgat tgtttcgatt
    12361 gcgattcgcg tagtatcgaa gcgttttcgc aacatcagta aaaacatgca gaacaccatg
    12421 gggcaggtga ccaccagcgc tgaacaaatg ctgaaaggcc ataaagaagt attgattttc
    12481 ggtggtcagg aagtggaaac gaaacgcttc gataaagtca gcaaccgaat gcgtcttcag
    12541 gggatgaaaa tggtttcagc ctcttccatc tctgatccga tcattcagct gatcgcctct
    12601 ttggcgctgg cgtttgttct gtatgcggcg agcttcccaa gtgtcatgga tagcctgact
    12661 gccggtacga ttaccgttgt tttctcttca atgattgcac tgatgcgtcc gctgaaatcg
    12721 ctgactaacg ttaacgccca gttccagcgc ggtatggcgg cttgtcagac actgtttacc
    12781 attctggaca gtgagcagga gaaagacgaa ggtaagcgcg tgatcgagcg tgcgactggc
    12841 gacgtggaat tccgcaacgt cacctttact tatccgggac gtgacgtacc cgcattgcgt
    12901 aacatcaacc tgaaaattcc ggcagggaaa acggtcgctc tggttggacg ctctggttca
    12961 ggtaaatcaa ccatcgccag cctgatcacg cgtttttacg atattgatga aggcgaaatc
    13021 ctgatggatg gtcacgatct gcgcgagtat accctggcgt cgttgcgtaa ccaggttgct
    13081 ctggtgtcgc agaatgtcca tctgtttaac gatacggttg ctaacaacat tgcttacgca
    13141 cgaactgaac agtacagccg tgagcaaatt gaagaagcgg cgcgtatggc ctacgccatg
    13201 gacttcatca ataagatgga taacggtctc gatacagtga ttggtgaaaa cggcgtactg
    13261 ctctctggcg gtcagcgtca gcgtattgct atcgctcgag ccttgttgcg tgatagcccg
    13321 attctgattc tggatgaagc tacctcggct ctggataccg aatccgaacg tgcgattcag
    13381 gcggcactgg atgagttgca gaaaaaccgt acctctctgg tgattgccca ccgcttgtct
    13441 accattgaaa aggcagacga aatcgtggtc gtcgaggatg gtgtcattgt ggaacgcggt
    13501 acgcataacg atttgcttga gcaccgcggc gtttacgcgc aacttcacaa aatgcagttt
    13561 ggccaatgat cgaaaaaatc tggtctggtg aatccccttt gtggcggcta ttgctgccac
    13621 tctcctggtt gtatggcctg gtgagtggcg cgatccgtct ttgctataaa ctaaaactga
    13681 agcgcgcctg gcgtgccccc gtaccggttg tcgtggttgg taatctcacc gcaggcggca
    13741 acggaaaaac cccggtcgtt gtctggctgg tggaacagtt gcaacagcgc ggtattcgcg
    13801 tgggggtcgt atcgcgggga tatggtggta aggctgaatc ttatccgctg ttattgtcgg
    13861 cagataccac cacagcacag gcgggtgatg aacctgtgtt gatttatcaa cgcactgatg
    13921 cgcctgttgc ggtttctccc gttcgttctg atgcggtaaa agccattctg gcgcaacacc
    13981 ctgatgtgca gatcatcgta accgacgacg gtttacagca ttaccgtctg gcgcgtgatg
    14041 tggaaattgt cgttattgat ggtgtgcgtc gctttggcaa tggctggtgg ttgccggcgg
    14101 ggccaatgcg tgagcgagcg gggcgcttaa agtcagttga tgcggtaatc gtcaacggcg
    14161 gtgtcccccg cagcggtgaa atccccatgc atctgctgcc gggtcaggcg gtgaatttac
    14221 gtaccggtac gcgttgtgac gttgctcagc ttgaacatgt ggtggcgatg gcagggattg
    14281 ggcatccgcc gcgctttttt gccacgctga agatgtgcgg cgtacaaccg gaaaaatgtg
    14341 taccgctggc cgatcatcag tctttgaacc atgcggatgt cagcgcgttg gtaagcaccg
    14401 ggcaaacgct ggtaatgact gaaaaagatg cggtgaaatg ccgggccttt gcagaagaaa
    14461 attggtggta tttgcccgtt gacgcacagc tttcaggtga tgaaccagcg aaactgcttg
    14521 cgcaactaac ctcgctggct tctggcaact agttacgccg cggcagcgtt cgattgatgg
    14581 agtcatgaat gtcgctgccg cacctctccc ttgctgatgc gcgtaatctt caccttgccg
    14641 cgcaaggcct gttaaacaaa ccccgccgtc gagcgtcgtt ggaggatatt ccggcaacga
    14701 tctcccgcat gtccttgctg caaatcgata ccatcaatat tgttgcccgt agcccatatc
    14761 tggtgctttt cagtcgtctg ggaaattatc ctgcccagtg gctggatgag tctctggcgc
    14821 gtggcgaatt aatggaatac tgggcgcatg aagcctgctt tatgccacgc agtgactttc
    14881 gtcttattcg ccaccgcatg ctggcacctg aaaaaatggg ctggaaatac aaagacgcct
    14941 ggatgcagga acatgaggcg gaaattgcac agttaattca gcatattcat gataaggggc
    15001 cggtacgttc agccgatttt gagtatcctc gtaaaggtgc aagcggctgg tgggaatgga
    15061 agccgcataa acggcatctg gaaggtttat ttactgccgg aaaggtgatg gtgattgaac
    15121 ggcgcaactt ccagcgcgtt tatgatttaa cccaccgtgt catgcctgac tgggatgatg
    15181 agcgcgatct cgtttcgcaa acagaagcag aaatcatcat gctggataac agtgcgcgta
    15241 gccagggaat attccgcgaa cagtggctgg cagattacta tcggctgaaa cgtccggcac
    15301 tggcagcgtg gcgcgaagcg agggctgaac agcggcaaat cattgctgtg catgttgaaa
    15361 aattgggcaa tctttggctg catgctgatt tgctgccgct actcgagcga gcgctggccg
    15421 gaaagctcac tgcaacgcac agcgcggtac tttcgccttt tgatcctgtt gtctgggatc
    15481 gcaaacgcgc agagcagctt tttgatttta gctaccggct ggagtgctat accccagcgc
    15541 cgaaacgcca gtatggctat tttgttctgc cgttattaca tcgtgggcaa ttagttgggc
    15601 gaatggatgc caaaatgcat cgccagacag gcatccttga agttatctct ctgtggttac
    15661 aggaaggtat taaaccaacg acaacgctgc aaaaagggtt acgtcaggcg attactgatt
    15721 tcgctaactg gcagcaggca acgcgggtga cattaggaca ctgcccgcaa ggtctcttta
    15781 cggattgccg caccggctgg gaaatagacc ccgtcgcata aatgattatg ataagattta
    15841 tgcattcata acccgatcca tctggaggaa ctatggatca tcgtctgctt gaaatcattg
    15901 cctgcccggt ttgcaacgga aaactttggt ataaccagga aaaacaagaa cttatttgca
    15961 aactggacaa cctggctttc ccgctgcgcg atggcattcc agtgttgctg gaaacggaag
    16021 cccgcgtgct gactgctgat gagagtaaat catgagtttt gtggtcatta ttcccgcgcg
    16081 ctacgcgtcc acgcgtctgc ccggtaaacc attggttgat attaacggca aacccatgat
    16141 tgttcatgtt cttgaacgcg cgcgcgaatc aggtgccgag cgcatcatcg tggcaaccga
    16201 tcatgaggat gtcgcccgcg ccgttgaagc cgctggcggt gaagtatgta tgacgcgcgc
    16261 cgatcaccag tcagggacag aacgcttggc ggaagttgtc gaaaaatgcg cgttcagcga
    16321 cgacacggtg atcgttaatg tgcagggtga tgaaccgatg atccctgcga caatcattcg
    16381 tcaggttgct gataacctcg ctcagcgtca ggtgggtatg gcgactctgg cggtgccaat
    16441 ccacaatgcg gaagaagcgt ttaacccgaa tgcggtgaaa gtggttctcg acgctgaagg
    16501 gtatgcactg tacttctcac gcgctaccat tccgtgggat cgtgatcgtt ttgcaaaagg
    16561 ccttgaaacc gttggcgata acttcctgcg tcatcttggt atttatggct accgtgcagg
    16621 ctttatccgt cgttacgtca cctggcagcc aagcccgtta gaacacatcg aaatgttaga
    16681 gcagcttcgt gttttgtggt acggcgaaaa aatccatgtt gctgtcgcgc atgaagttcc
    16741 tggcacaggt gtggataccc ctgaagatct cgagcgcgtt cgcgctgaaa tgcgctaatt
    16801 tcacttcacg acacttcagc caattttggg aggagtgtcg taccgttacg attttcctca
    16861 atttttcttt tcaacaattg atctcattca ggtgacatct tttatattgg cgctcattat
    16921 gaaagcagta gcttttatga gggtaatctg aatggaacag ctgcgtgccg aattaagcca
    16981 tttactgggc gaaaaactca gtcgtattga gtgcgtcaat gaaaaagcgg atacggcgtt
    17041 gtgggctttg tatgacagcc agggaaaccc aatgccgcta atggcaagaa gctttagtac
    17101 gcccggaaaa gcccgacaac tggcatggaa aaccaccatg ctggcaagaa gtgggactgt
    17161 ccgtatgccg actatttatg gtgtgatgac gcatgaagaa caccccggcc ctgatgtcct
    17221 gctactggag cggatgcgtg gtgtttcggt agaggcacca gcccgaacac cagaacgttg
    17281 ggaacagctc aaagaccaaa tcgttgaagc cttactggcc tggcaccgtc aggacagtcg
    17341 cggttgcgtc ggcgcggtcg acaatactca ggaaaatttc tggccctcat ggtaccggca
    17401 acatgttgaa gtgctatgga ccacgctcaa tcagttcaat aacaccggtc tgacgatgca
    17461 ggataagcgg atcctgtttc gcactcgcga atgtctcccg gcattatttg aaggttttaa
    17521 cgacaattgt gtgctgattc acggtaactt ctgtttacgc agcatgttga aagattcgcg
    17581 cagcgatcag ttactggcga tggtcgggcc gggactaatg ctttgggcac cgcgagaata
    17641 cgaactgttc cgactaatgg ataattctct ggcggaagat ttgctctgga gttacctgca
    17701 acgcgcgcca gtggcggagt cgttcatctg gcggcgttgg ttgtatgtgt tatgggatga
    17761 agttgcgcaa ctggttaata ccggacgatt tagtcggcgc aacttcgatc tggcatcaaa
    17821 atcactcttg ccgtggctcg cctgacgaac cttttagcca ctgccagatg cggccaagcg
    17881 tttcatagcc aacacgatcg ctatgcatca accagaccgg agaagggatt gcccgttccc
    17941 acgggttaag cggcgagtcg atggccaact gattcgccgg ggctggtagt ggatttaacc
    18001 cttcctgctg aaaaaagatc atcgcccgcg gcagatgtga tgcggaggtc accagcagga
    18061 aaggggcgtc accaatcgcc tgtttcactg ccgcagcttc ttcttcggta tcttttggca
    18121 aatccagggt gataatttgc tcgcgcggta cgcccagcga ttgcgcaact ctggcaccta
    18181 cttccgctgt actcaccgta ttggtttttg ctacgcctcc cgtgaagatc agttttgaac
    18241 ccggattttc gcgccataaa cggataccct cattcaggcg tggcagactg ttattgatta
    18301 aattagagct cggtgcccac tgcgggttcc aggtataacc acctccgagc accacgatat
    18361 agtccacttt ttgcgaatta ttccatgtcg gataggtgct ttcgatggga cgcagcaggc
    18421 ggtcggctac cggttgcaaa ctcaataaca agagtgccag ccacccaatg ctaataaaaa
    18481 tcttcccggt tttttgaaaa cggctaaacc acagtagcgc caggccagcg ccaataatta
    18541 acaacatcag cggaagtggc agcaacatat tgccaatcac ttttttcagt gtaaaaagca
    18601 tcctttctgg ttcctttttt aaccatatag caagggatcg ccagcgatat tacaccagac
    18661 gggttcattc tcgccgtggc tgtgacaaaa tagcgtcttt atcagaaaac gtcttagcca
    18721 accggtggag aaagcaatgc aggatcgcaa ttttgatgat attgcggaaa agttttcccg
    18781 taacatttac ggcaccacca aagggcagct tcgacaggct attctgtggc aggatctcga
    18841 tcgcgtgctg gcggaaatgg gtccgcaaaa actgcgtgtg ctggatgcgg gcggtggaga
    18901 agggcagacc gcaatcaaaa tggccgagcg cgggcatcag gtcattttat gcgatctttc
    18961 tgcgcagatg atcgaccgcg caaaacaggc ggcagaagca aaaggtgtga gcgacaacat
    19021 gcaatttata cattgcgccg ctcaggatgt tgcttcgcat ttggaaacgc ccgttgatct
    19081 gatattgttc catgcggtgc tcgagtgggt ggctgatccc cgcagcgtat tgcagaccct
    19141 gtggtcagtg ttgcgtccag gcggcgtgtt gtcgttaatg ttctacaatg cgcatggttt
    19201 gttgatgcat aacatggtcg ccgggaattt tgattacgtg caggcgggaa tgccgaaaaa
    19261 gaaaaaacgg acgctttcgc cagattatcc acgcgacccg acgcaggttt atctgtggct
    19321 ggaagaagct ggttggcaaa ttatgggtaa gacaggcgtt cgcgtgtttc atgattatct
    19381 gcgcgagaag caccagcagc gcgactgcta tgaagcatta cttgaattag aaacgcgtta
    19441 ttgccgtcag gaaccgtata ttaccctggg gcgttatatt catgtcaccg cgcgcaaacc
    19501 gcagagcaag gataaagtat gagtgaattt tcccagacag tccccgaact ggttgcctgg
    19561 gccagaaaaa atgacttctc catttcgctg ccggtagacc gactctcttt tctgctggca
    19621 gttgccacgc tgaacggcga gcgtctggat ggtgagatga gtgaaggcga gctggtggat
    19681 gcattccgcc atgtgagtga tgcgtttgag caaaccagcg aaaccatcgg cgtgcgcgcc
    19741 aacaacgcga tcaacgacat ggtgcgtcaa cgtctgctga accgctttac cagcgagcag
    19801 gcagaaggga acgcaattta ccgtctgacg ccgctcggca tcggcattac tgactattac
    19861 atccgtcagc gcgagttttc tacgctgcgc ctttctatgc agttgtcgat tgtggcgggt
    19921 gagctcaaac gcgcagcaga tgccgccgaa gagggcggtg atgaatttca ctggcaccgt
    19981 aatgtctatg cgccactgaa atattcggta gcagaaattt tcgacagtat cgacctgacg
    20041 caacgtctga tggacgaaca gcagcagcag gtgaaggacg atatcgccca gttgctgaac
    20101 aaagactggc gggcggcgat ttccagctgt gaattgttgc tttcggaaac ttccggaacg
    20161 ctgcgtgaat tgcaggatac actggaagcg gcaggcgaca aattgcaggc taatctgttg
    20221 cgcattcagg atgcgacgat gactcatgac gatctgcatt tcgtcgatcg tctggtgttc
    20281 gatctgcaga gcaaactcga ccgtattatc agttggggcc agcaatccat cgatttgtgg
    20341 attggctacg accgccacgt acataaattt attcgtaccg cgatcgatat ggataaaaac
    20401 cgcgtctttg ctcagcggtt acgtcagtcg gtacaaacct attttgatga gccgtgggcg
    20461 ctaagttatg ccaatgccga tcgtctgctg gatatgcgtg acgaagagat ggcactgcgc
    20521 gatgaagaag tgactgggga acttccgcca gatttggaat acgaagagtt taacgagatc
    20581 cgcgaacagc tggcggcgat catcgaagaa caacttgccg tgtacaaaac cagacaagtg
    20641 ccgctggatc ttggtctggt ggtacgcgaa tatctgtcac agtatccgcg tgcacgtcac
    20701 tttgacgttg cgcgcattgt tattgatcag gcggtacgtc ttggcgtagc gcaagcagat
    20761 ttcaccggac tgccagcgaa atggcagccg attaatgatt acggagccaa ggtacaggcg
    20821 catgtcatcg acaaatattg aacaagtgat gccggttaag ctggcgcagg cgctggcgaa
    20881 tccgttattt ccggcgctgg acagcgcctt acgttcaggc cgccatattg gcctcgacga
    20941 actggataat catgcattcc tgatggattt tcaggaatat ctggaagagt tttacgcgcg
    21001 ttataacgtt gagcttattc gtgcaccgga agggttcttc tatttacgtc cacgttccac
    21061 cacgctgatc cctcgttccg tcttgtcgga actggatatg atggtcggga aaatcctctg
    21121 ttatctctat ctcagcccgg aacggctggc gaatgagggg attttcaccc agcaggaact
    21181 gtacgacgaa ctgctcaccc tggccgatga agcgaaactg ctgaaactgg tgaacaaccg
    21241 ttcaaccggt tcagacattg accgtcagaa gttgcaggaa aaagtacgtt cttcgctcaa
    21301 ccgtctgcgt cgtttaggta tggtgtggtt tatgggccac gacagcagca agttccgcat
    21361 taccgaatcg gtgttccgct tcggagccga tgtgcgtgct ggcgacgatc cccgtgaagc
    21421 acagcgtcgc ctgattcgtg atggcgaagc aatgccgatt gaaaatcatc tgcaactcaa
    21481 cgatgaaacc gaagagaatc agccagatag cggagaggaa gaataatgat tgaacgcggt
    21541 aaatttcgct cactgacgct gattaactgg aacggcttct ttgcccgaac ttttgacctt
    21601 gacgagctgg tcacgacgct ttctggcggt aacggggcgg gtaaatccac caccatggcg
    21661 gcgttcgtta cggcgctgat ccccgacctg accctgctgc atttccgtaa cactacggaa
    21721 gccggggcca ccagcggttc gcgcgataaa ggtctgcacg gtaagctgaa agcgggtgtc
    21781 tgttattcga tgctcgacac cattaactcg cgccaccagc gcgtggtggt cggtgtgcgt
    21841 ctgcaacagg ttgccggacg cgatcgtaaa gtggatatca aaccatttgc cattcaggga
    21901 ctgccgatgt cggtgcagcc gacacagctg gtgaccgaaa ctctgaacga acgccaggcg
    21961 cgcgtgctgc cgcttaacga gctgaaagac aagctcgagg cgatggaagg cgtgcagttt
    22021 aaacagttca actccattac cgattaccac tcgctgatgt tcgatctggg catcattgcg
    22081 cgtcgtctgc gctccgcatc tgaccgtagc aaattctatc gtctgatcga agcttcgctg
    22141 tatggcggga tctccagtgc cattacccgt tctctgcgcg actacctgtt gccggaaaac
    22201 agcggcgtgc gtaaagcgtt ccaggacatg gaagcggcgc tgcgtgaaaa ccgcatgacg
    22261 ctggaagcga ttcgtgtcac ccagtcggac cgtgacctgt ttaagcatct gatcagcgaa
    22321 gctaccaact acgtggcggc ggactacatg cgtcacgcca acgagcgccg tgtccatctc
    22381 gataaggccc tggagtttcg tcgcgaacta catacttcgc gtcagcaact ggcggcggag
    22441 cagtacaaac acgtcgatat ggcgcgtgag ctggcagagc acaacggtgc cgaaggcgat
    22501 ctggaagcgg attatcaggc ggccagcgat cacctgaacc tggtgcaaac cgcactgcgt
    22561 cagcaggaga agatcgaacg ctacgaagcg gatctcgatg agctgcagat ccgtctggaa
    22621 gagcaaaacg aagtggtggc agaagccatc gaacgccagg aagagaacga ggctcgcgcg
    22681 gaagctgccg aactggaagt ggacgagctg aaaagccagc ttgctgacta ccagcaggcg
    22741 ctggacgtcc agcaaacgcg cgcgatccag tacaaccagg cgattgctgc gcttaatcgt
    22801 gccaaagaac tgtgccatct gccggactta accgccgaca gcgccgccga atggctggaa
    22861 accttccagg cgaaagagct ggaagcgact gaaaaaatgc tctctcttga gcagaaaatg
    22921 agcatggcgc aaaccgcgca cagccagttt gagcaggctt atcagctggt ggtggcaatc
    22981 aacggcccgc tggcgcgtaa cgaggcgtgg gatgtcgctc gcgaactatt gcgcgaaggg
    23041 gtcgatcagc gtcacctggc agagcaggtt cagccgttgc ggatgcgctt aagcgaactg
    23101 gaacagcgtc tgcgcgaaca gcaagaagct gagcgtctgc tggcagattt ctgcaaacgt
    23161 cagggcaaga attttgacat tgacgagctg gaagccctgc atcaggagct ggaagcacgc
    23221 attgcctctc tttccgatag cgtgtctaac gcccgtgaag agcggatggc actgcgccag
    23281 gagcaggaac agctgcagtc tcgcattcag agtttgatgc agcgtgcacc ggtttggctg
    23341 gcagcgcaaa acagtctcaa ccagttgagc gaacagtgcg gcgaagagtt cacctccagc
    23401 caggacgtca ctgaatatct gcaacagttg ctggagcgtg agcgcgaggc cattgttgaa
    23461 cgcgatgaag taggcgcgcg caaaaacgcc gtcgatgaag agatcgaacg tttaagccag
    23521 cctggcggct ctgaagatca gcgtttgaac gcgctagcgg agcgttttgg tggtgtgctg
    23581 ctgtcagaaa tttatgacga cgttagcctg gaagatgcgc cgtacttctc agcgctgtat
    23641 ggcccgtcac gccacgccat cgtggtgcca gatctgtcac aggtaaccga acacctggaa
    23701 ggtttgaccg attgcccgga agatctctat ttgatcgaag gggatccgca gtcattcgat
    23761 gacagcgtgt tcagcgttga tgagctggaa aaagcggtag tggtgaaaat cgccgatcgt
    23821 cagtggcgtt attcacgttt cccggaagtg ccgctgtttg gtcgtgctgc gcgtgaaagc
    23881 cgtattgaaa gcctccatgc cgagcgtgaa gtgctttccg aacgcttcgc cacgctctcc
    23941 tttgatgtac agaaaaccca gcgtctgcat caggcgttca gccgctttat cggcagtcat
    24001 ctggcggtgg cgtttgagtc tgacccggaa gcagaaatcc gtcaactgaa cagccgtcgc
    24061 gtcgaactgg agcgggcgtt gagtaatcat gaaaatgata accagcagca gcgtattcag
    24121 tttgagcagg cgaaagaagg cgttacggcg ctgaaccgca ttctgccgcg tctcaacctg
    24181 ttggctgatg acagcctggc ggatcgcgtc gatgaaatcc gcgaacgtct ggatgaagcc
    24241 caggaagccg cgcgttttgt tcagcagttt ggcaatcaac tggcgaaact ggaaccgatc
    24301 gtttcggtat tgcagagcga cccggaacag ttcgaacagt taaaagaaga ttacgcgtac
    24361 tctcagcaga tgcagcgcga tgcccgtcag caggcgtttg ccctgacgga agtggtgcag
    24421 cgtcgtgcgc actttagcta ctctgactcg gcagaaatgc ttagcggtaa cagcgatctc
    24481 aacgaaaaac tgcgtgaacg tctggaacag gcggaagcgg agcgtacccg cgctcgcgaa
    24541 gcgttgcgcg gtcacgcagc gcagttgagt cagtacaacc aggtgctggc ttcgctgaaa
    24601 agttcttacg acaccaaaaa agagctactc aacgatctgc aacgtgaatt gcaggatatc
    24661 ggcgtgcgtg ctgatagcgg ggcagaagag cgggcacgta ttcgccgtga cgagctgcat
    24721 gcacaactga gcaataaccg ttcgcgccgc aatcaactgg aaaaagcgct caccttctgc
    24781 gaagcggaga tggataacct gacccgcaaa ctgcgcaagc tggagcgtga ttactttgag
    24841 atgcgcgagc aggtagtgac cgcgaaagcg ggctggtgtg cagtcatgcg catggtgaaa
    24901 gataacggcg ttgagcgccg cttacaccgt cgtgagctgg cttatctctc cgctgatgat
    24961 ttgcgttcca tgtcggataa ggcgttaggt gcgctgcgtc tggcggtggc ggataacgaa
    25021 catctgcgcg acgtgctgcg catgtcggaa gatcccaaac gtccggagcg taaaattcag
    25081 ttcttcgtgg cggtttatca gcatctgcgt gaacgtattc gtcaggatat tattcgtacc
    25141 gatgatccgg tggaagctat cgaacagatg gagatcgaac ttagccgtct gaccgaagag
    25201 ttaacctccc gtgaacagaa actggcgatc agttcccgca gcgtggcgaa catcattcgc
    25261 aaaaccattc agcgcgagca gaaccgtatc cgtatgctca accaggggtt gcagaacgta
    25321 tcgtttggtc aggtgaacag cgtgcgcctc aacgtgaacg tgcgtgaaac gcacgccatg
    25381 ctgctggatg tgctctctga acagcatgag cagcatcagg atctgtttaa cagcaaccgt
    25441 ctgaccttct cggaagcgct ggcgaaattg tatcagcgtc ttaatccgca gattgatatg
    25501 gggcagcgca cgccgcagac cattggcgaa gaactgctgg attaccgcaa ctatctggaa
    25561 atggaagttg aggttaaccg tggttccgat ggctggttgc gcgcagagtc tggtgcattg
    25621 tcgaccggtg aggcgattgg taccggtatg tcgattctgg tgatggtggt acaaagctgg
    25681 gaagatgaat ctcgccgcct gcgcggtaaa gatatctctc cttgccgcct gctgttcctc
    25741 gatgaagcag cgcgactgga tgctcgttct atcgccacgc tgtttgaatt gtgtgagcgt
    25801 ttgcaaatgc agctcatcat cgcagcgccg gaaaatatca gcccggagaa aggcaccacc
    25861 tataaactgg tgcgtaaagt cttccagaat accgaacatg ttcatgtcgt cggcctgcga
    25921 ggatttgcgc cgcaactccc tgaaacgctt ccaggaagtg acgaagcgcc ttctcaggcg
    25981 agttaaaatt aaggcggcag caatgccgcc ttttcttttt ccgaaaactc cgtttctgca
    26041 ctaaaaaagt ggcacattac ggcgcgcttt tctttaaact tctttacatt aggttatgta
    26101 aaaacgtatc ggcgtttata tactgaagat aagcctgatg agtaacaggc ttgctcgtca
    26161 tactttcgtg agtattggcg ttgtacaggc aagtcgtaaa ataacagcct ggctattcag
    26221 agtatgataa aaacaggggg caagggatgt tgcttaatat gatgtgtggt cgtcggctgt
    26281 cggcaatcag tttgtgcctg gccgtaacat tcgctccact gttcaatgcg caggccgatg
    26341 agcctgaagt aatccctggc gacagcccgg tggctgtcag tgaacagggc gaggcactgc
    26401 cgcaggcgca agccacggca ataatggcgg ggatcctgcc attgcctgaa ggtgcggcag
    26461 aaaaagcccg cacgcaaatc gaatctcaat tacccgcagg ttacaagccg gtttatctta
    26521 accagcttca actgttgtat gccgcacgcg atatgcaacc catgtgggaa aaccgtgatg
    26581 ctgttaaagc cttccagcaa cagctggcag aggtggcgat tgccggtttc cagccgcagt
    26641 ttaataaatg ggtagagtta ctgaccgatc ctggtgttaa cgggatggca cgcgacgtgg
    26701 tgctctctga tgcgatgatg ggctatctcc atttcattgc aaatattccg gtcaaaggca
    26761 ctcgctggct atatagcagt aaaccttatg cgcttgcaac gccgccgctt tcggtgatta
    26821 accaatggca gcaggcgctg gataaaggtc aattgcctac gtttgttgca ggactggcac
    26881 cgcagcatcc gcaatatgcg gtgatgcatg aatcgttact ggccttactc agtgacacca
    26941 aaccgtggcc ccaactgacc ggcaaagcaa cgttgcgccc agggcagtgg agtaacgacg
    27001 taccggcgtt gcgtgaaata ttgcaacgca caggcatgtt ggacgggggg ccgaaaatta
    27061 ctctacctgg cgatgacacg ccaactgacg cggtagtcag cccatccgct gttactgttg
    27121 aaacagcaga aactaagccg atggataagc aaacgacgtc tcgtagtaaa cctgcgcctg
    27181 ccgttcgcgc cgcctacgat aatgaactgg tggaagccgt taaacgtttt caggcatggc
    27241 aaggattggg ggcagatggt gctattggcc cggcaacgcg tgactggtta aacgtaacgc
    27301 ccgcccagcg tgctggcgtg ttggctctca acatccagcg attgcgcttg ctgccaacag
    27361 agctttctac cgggatcatg gttaacattc cggcctattc gctggtctac tatcagaacg
    27421 gcaatcaggt gctggattcg cgagtcattg tcggtcgccc cgatcgcaaa acgccgatga
    27481 tgagcagtgc ccttaacaat gtagtggtaa acccgccgtg gaacgtacct ccaactctgg
    27541 cacgcaaaga tattctgccg aaagtgcgca acgatccggg atatctcgaa agccatggct
    27601 atacggtgat gcgcggctgg aacagcagag aagcgattga cccatggcag gttgactggt
    27661 ctacaatcac ggcctcgaat ttaccgtttc gcttccaaca ggctccaggc ccacggaact
    27721 cgctggggcg ctataaattc aatatgccga gttcagaggc catttatttg catgacacgc
    27781 cgaaccacaa tctgttcaag cgtgatacac gcgcattgag ctcaggctgt gtacgagtga
    27841 ataaagcttc cgatctggcg aatatgctgt tgcaggatgc aggctggaat gacaaacgta
    27901 tttctgatgc gctgaagcag ggtgatacac gttacgtcaa tattcggcag tcgattccgg
    27961 tgaatctcta ctacctgacg gcctttgttg gtgcagatgg tcgtacccag tatcgtacag
    28021 atatttacaa ttatgatctg cctgcgcgat ccagctcgca aatcgtatcg aaagcggaac
    28081 aattaatcag gtaaatgaag tagttcgggg aaatcagttg tcgtaataat tagcatgatt
    28141 gggggcaatt ctctgcagcc cccgtcactg ctggggttga gtcatcttga cgtctgcttt
    28201 acgggcggtt aaggtgcctc ttgtgcgcca gaagtgcata taaacgataa cattgacctg
    28261 tagacttgat tatcatggac aaattcgacg ctaatcgccg caaattgctg gcgcttggtg
    28321 gcgttgcact cggtgccgcc atcctgccga cccctgcgtt tgcaacactc tctaccccac
    28381 gcccgcgcat tttgacactc aataatcttc ataccggaga gtcaatcaaa gcggagtttt
    28441 tcgatggcag aggctatatt caggaagaat tggcaaaact taaccatttt ttccgcgatt
    28501 accgcgcgaa caaaataaag tccatcgacc caggattatt cgaccagttg tatcgcctgc
    28561 aagggttatt aggcacccgc aaaccggtgc aactcatttc cggttatcgt tccattgata
    28621 ccaacaatga actacgcgcc cgcagccgtg gagtagcgaa gaaaagctat cacactaaag
    28681 gccaggcgat ggatttccat attgaaggta tcgcgttaag caatattcgc aaagccgcgt
    28741 tatctatgcg cgcaggtggt gtaggatatt acccacgtag taactttgtg catattgata
    28801 ccgggccagc acggcactgg tagtaattgc ttaacgaaac aggggcagta tgaactatcg
    28861 tattattccg gtcaccgcat tctcccagaa ctgttcattg atctggtgtg aacaaacccg
    28921 cctggccgca ctggtcgatc ctggcggcga tgcggaaaaa atcaaacagg aagttgacgc
    28981 cagcggcctg acactgatgc agatcctgct gacgcatggt catctggacc acgttggcgc
    29041 agcggcggaa ctggcgcaac attacggcgt gccggttttt ggcccggaaa aagaagatga
    29101 gttctggctg caaggcttgc ctgcgcaaag tcgtatgttt ggtctggaag agtgccagcc
    29161 gctgacgcca gatcgttggc tgaacgaagg cgataccatc agcataggga atgtgacttt
    29221 acaggtgtta cattgccctg ggcatacgcc gggtcatgtc gtgttttttg atgatcgggc
    29281 aaagctgctg atttctggcg atgttatttt caaaggcgga gtagggcgca gtgacttccc
    29341 gcgtggcgat cataatcaac tgatttcttc aatcaaagat aaattgctgc cactgggtga
    29401 tgacgtgaca tttattccgg gtcacggacc attatccaca cttggttatg aacgcctgca
    29461 taatcccttc ctgcaagacg aaatgcccgt ctggtaaggc acacataaaa aagcccgctt
    29521 ttcagcgggc ttcattgttt ttaacgctta cagcactgcc acaatcgctt cgcacagcgg
    29581 agccatgtta tctggtgtca tcccggccac gtttacgcga ccagaagcaa cagcatacac
    29641 gccaaactct tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat
    29701 gccgttctgt ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt
    29761 gacgaacaac tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca
    29821 aatcgcacgt aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg
    29881 tgggttagag tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt
    29941 ttcactgtcg gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt
    30001 tttagagtag gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc
    30061 ttcagcatct tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa
    30121 ccagcctttc tcaacggaga gttgtgccag tgtctgccat tgttccagcg tagggtcgat
    30181 accggttggg ttgtggcagc agccatggaa cagcactacg tcgccagcct gagcttcgtt
    30241 caggctgtta atcagtgcat cgaagtcaag ggtgtggttt tccgcatcat aataagcgta
    30301 ttcacgaact tccagatctg cagagttaaa gacgctctta tggttcggcc agcttgggtt
    30361 gctcacccac actcgcttaa cgctggtatt ttttgccagg aaatcggcag ctatgcgtag
    30421 tgcgccagtg ccacccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct
    30481 acctttacca aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc
    30541 gaggtaattt ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt
    30601 cagtaccggg gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc
    30661 gggacgttca tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc
    30721 ggtaatgttc tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca
    30781 gggtaacggg agatttacaa aattccaact attactgatg aaaacgcagg ctgtttttgc
    30841 aagacgtgag attgctctgg aaggcataaa aaaaacagga ccaaagtcct gttttttcgg
    30901 catttaacaa agaggtgtgc tattagaact ggtaaacgat acccacagca acggtgtcgt
    30961 ctgaacctac gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg
    31021 tggacatgtt tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa
    31081 catcaccgat accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca
    31141 gaccgaaatc gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc
    31201 cgctgatgtt tgtaaattta ttagtgatcg gcgtagcgtt acgcgtttca ccgtagttcg
    31261 ctgccaggta gatgttgttc gcatcatact tcagaccagt agcccactgt tcagcttttt
    31321 taccgttgcc aagaagttga gcttcttgca ggttggtacg gtcagctgca ccataagcac
    31381 caacgatacc aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag
    31441 agcggcgtgc agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat
    31501 caaccagacc aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga
    31561 agaagtcatc gctgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca
    31621 gtgcatcata aaccacaccg tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt
    31681 taagacccgc gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt
    31741 tgttaccctg gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgag
    31801 tttccccttt aaaaccaaga cgggcatagg tcatgtcgcc attgccaccg taactgtttt
    31861 caccgttacc cttggagaaa taatgcagac cgacagcttt accgtacaga tctactttgt
    31921 tgccatcttt gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga
    31981 cgatcactgc cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg
    32041 acacctgcca ctgccgtcaa taatttctgt caataaaatt ttacg
//
LOCUS       DBFIFS010000034        31767 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.034, whole genome shotgun sequence.
ACCESSION   DBFIFS010000034 DBFIFS010000000
VERSION     DBFIFS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.034"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..2084
                     /locus_tag="N7A07_001434"
     CDS             <1..2084
                     /locus_tag="N7A07_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2476124.1"
                     /translation="EMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDE
                     SGQVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAV
                     DKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGDYNIID
                     SVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTR
                     VFRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSN
                     KLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKL
                     CQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSA
                     ICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAME
                     IGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQI
                     IKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVE
                     ITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTG
                     DIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFS
                     AWDKSGKLVKE"
     gene            2092..3420
                     /locus_tag="N7A07_001435"
     CDS             2092..3420
                     /locus_tag="N7A07_001435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2476125.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3490..3819)
                     /locus_tag="N7A07_001436"
     CDS             complement(3490..3819)
                     /locus_tag="N7A07_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2476126.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3809..4195)
                     /locus_tag="N7A07_001437"
     CDS             complement(3809..4195)
                     /locus_tag="N7A07_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2476127.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(4421..5746)
                     /gene="rimO"
                     /locus_tag="N7A07_001438"
     CDS             complement(4421..5746)
                     /gene="rimO"
                     /locus_tag="N7A07_001438"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU2476128.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5959..6342
                     /gene="bssR"
                     /locus_tag="N7A07_001439"
     CDS             5959..6342
                     /gene="bssR"
                     /locus_tag="N7A07_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU2476129.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            6453..7568
                     /gene="yliI"
                     /locus_tag="N7A07_001440"
     CDS             6453..7568
                     /gene="yliI"
                     /locus_tag="N7A07_001440"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU2476130.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7565..8191)
                     /gene="gstB"
                     /locus_tag="N7A07_001441"
     CDS             complement(7565..8191)
                     /gene="gstB"
                     /locus_tag="N7A07_001441"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU2476131.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            8437..9639
                     /gene="dacC"
                     /locus_tag="N7A07_001442"
     CDS             8437..9639
                     /gene="dacC"
                     /locus_tag="N7A07_001442"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU2476132.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9686..10444)
                     /gene="deoR"
                     /locus_tag="N7A07_001443"
     CDS             complement(9686..10444)
                     /gene="deoR"
                     /locus_tag="N7A07_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU2476133.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(10502..11098)
                     /gene="ybjG"
                     /locus_tag="N7A07_001444"
     CDS             complement(10502..11098)
                     /gene="ybjG"
                     /locus_tag="N7A07_001444"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU2476134.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            11383..12615
                     /gene="mdfA"
                     /locus_tag="N7A07_001445"
     CDS             11383..12615
                     /gene="mdfA"
                     /locus_tag="N7A07_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU2476135.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(12656..12940)
                     /gene="ybjH"
                     /locus_tag="N7A07_001446"
     CDS             complement(12656..12940)
                     /gene="ybjH"
                     /locus_tag="N7A07_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU2476136.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(13026..13841)
                     /gene="ybjI"
                     /locus_tag="N7A07_001447"
     CDS             complement(13026..13841)
                     /gene="ybjI"
                     /locus_tag="N7A07_001447"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU2476137.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNREQFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13841..15049)
                     /locus_tag="N7A07_001448"
     CDS             complement(13841..15049)
                     /locus_tag="N7A07_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476138.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            15133..15669
                     /gene="rcdA"
                     /locus_tag="N7A07_001449"
     CDS             15133..15669
                     /gene="rcdA"
                     /locus_tag="N7A07_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU2476139.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15844..17529)
                     /locus_tag="N7A07_001450"
     CDS             complement(15844..17529)
                     /locus_tag="N7A07_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU2476140.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            17799..18176
                     /locus_tag="N7A07_001451"
     CDS             17799..18176
                     /locus_tag="N7A07_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU2476141.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(18206..18463)
                     /gene="grxA"
                     /locus_tag="N7A07_001452"
     CDS             complement(18206..18463)
                     /gene="grxA"
                     /locus_tag="N7A07_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU2476142.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            18623..18910
                     /locus_tag="N7A07_001453"
     CDS             18623..18910
                     /locus_tag="N7A07_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU2476143.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18894..19616
                     /gene="nfsA"
                     /locus_tag="N7A07_001454"
     CDS             18894..19616
                     /gene="nfsA"
                     /locus_tag="N7A07_001454"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU2476144.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            19677..20579
                     /gene="rimK"
                     /locus_tag="N7A07_001455"
     CDS             19677..20579
                     /gene="rimK"
                     /locus_tag="N7A07_001455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU2476145.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            20667..21142
                     /locus_tag="N7A07_001456"
                     /pseudo
     CDS             20667..21142
                     /locus_tag="N7A07_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
     gene            21493..22605
                     /gene="potF"
                     /locus_tag="N7A07_001457"
     CDS             21493..22605
                     /gene="potF"
                     /locus_tag="N7A07_001457"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU2476146.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            22700..23833
                     /gene="potG"
                     /locus_tag="N7A07_001458"
     CDS             22700..23833
                     /gene="potG"
                     /locus_tag="N7A07_001458"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU2476147.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            23843..24796
                     /gene="potH"
                     /locus_tag="N7A07_001459"
     CDS             23843..24796
                     /gene="potH"
                     /locus_tag="N7A07_001459"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU2476148.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            24793..25638
                     /gene="potI"
                     /locus_tag="N7A07_001460"
     CDS             24793..25638
                     /gene="potI"
                     /locus_tag="N7A07_001460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU2476149.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            25698..26186
                     /locus_tag="N7A07_001461"
     CDS             25698..26186
                     /locus_tag="N7A07_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU2476150.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            26227..27354
                     /gene="rlmC"
                     /locus_tag="N7A07_001462"
     CDS             26227..27354
                     /gene="rlmC"
                     /locus_tag="N7A07_001462"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU2476151.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            27634..28977
                     /locus_tag="N7A07_001463"
     CDS             27634..28977
                     /locus_tag="N7A07_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU2476152.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            28999..29319
                     /locus_tag="N7A07_001464"
     CDS             28999..29319
                     /locus_tag="N7A07_001464"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2476153.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            29331..30812
                     /locus_tag="N7A07_001465"
     CDS             29331..30812
                     /locus_tag="N7A07_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU2476154.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(30863..31594)
                     /gene="artJ"
                     /locus_tag="N7A07_001466"
     CDS             complement(30863..31594)
                     /gene="artJ"
                     /locus_tag="N7A07_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU2476155.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
BASE COUNT         7857 a         7482 c         8136 g         8292 t
ORIGIN      
        1 tggaaatgat gccattttta gactcggtgg gactggtact tgatgataat aaatattatc
       61 tcttttcgcg gagggcgaac gataaaatcg ttgtttatca tcaggaacaa gtaaatggac
      121 cgcttgtcga cgagtcaggg caggttattt ttgccgattt taacccatcg aaacgaccgt
      181 ggtcggtggc ttcagatgac tctaacaaca gctggaatcc ggcctacaat tgctttgatc
      241 gtccgggtaa aaaatgtatc tcttttacgc tacgcatcaa cggcaaagat cacgatttgt
      301 tagcggtgga taaaattcat gtcgatttaa actggcgata tctgaacgag tatcttgatc
      361 aaatcagcgc taatgatgaa gttctatttt tgaaacaagg ccatgagatc attgccaaga
      421 atcaactcgc tcgtgaaaaa ctgattattt ataatagcga aggtgattat aatattattg
      481 attctgtcga tactgaatat atcgaaaaaa catcagcggt gccaaataac gcattattcg
      541 aaatctattt ttattatccg ggcggtaatt tattgaacgc atcagataaa cttttttatc
      601 tgccgtttgc gttcattatt atcgtattgc tggtggttta tttaatgacc actcgtgtgt
      661 tccgtcggca attttctgaa atgacagagc tgattaatac gctggcgttt ttgcctgact
      721 caacggatca gatcgaggct ctgaaaattc gcgaaggcga tgcgaaagag attatcagca
      781 tcaaaaattc gatcgcggaa atgaaagatg ccgaaattga acggtcaaat aaattgctct
      841 cactgatctc ttacgatcag gaaagtggtt ttattaaaaa tatggcgatt attgagtcca
      901 acaataatca gtatctggct gtggggatca tcaaactgtg tggtctggaa gccgtggaag
      961 ctgtgtttgg tgttgatgaa cgcaataaaa tcgtcaggaa attgtgtcag cgaattgctg
     1021 agaaatatgc acaatgctgc gatatcgtga cattcaatgc cgatctctat ttacttctgt
     1081 gtcgggaaaa tgtacagaca tttacccgta aaatagcgat ggtaaacgat tttgacagca
     1141 gctttggcta ccgcaatctg cgcatccata agtctgccat ttgtgaacct ttgcaggggg
     1201 aaaacgcctg gagttacgca gaaaaactga aactggcgat ttccagtatc cgtgaccata
     1261 tgttctccga gtttattttc tgtgatgacg cgaaactcaa cgaaatagaa gagaatatct
     1321 ggattgcgcg taatattcgc catgcaatgg aaattggcga actattcctc gtctatcaac
     1381 cgatcgttga tattaacacc cgcgccattc tgggcgcgga ggcgttgtgc cgttgggtgt
     1441 ctgcggagcg ggggatcatt tcaccgctga agttcattac cattgctgaa gatatcgggt
     1501 ttatcaatga gctgggttat cagattatta aaacggcaat gggtgaattc agacatttta
     1561 gtcagcgtgc atcgctgaag gatgatttct tactgcatat taatgtttcg ccctggcagt
     1621 taaacgaacc gcactttcat gagcgtttta ccaccatcat gaaagaaaat ggcctgaagg
     1681 cgaacagcct ctgtgttgag atcactgaaa ccgtgatcga gcgaattaat gaacattttt
     1741 atctcaatat tgaacaactg cgtaaacaag ggttacggat atcgattgat gactttggca
     1801 ccggtttgtc aaacctgaaa cgtttttatg aaattaatcc agacagcata aaggtggact
     1861 cgcaatttac cggcgatatt ttcggtactg cgggaaaaat tgtgcgcatt attttcgacc
     1921 tggcacgcta taaccggatc ccggtgattg cggaaggcgt agagagcgaa gacgttgcgc
     1981 gcgaattaat caaattagga tgtgttcagg ctcaggggta tctgtatcag aaacccatgc
     2041 cattctccgc ctgggataaa agtggaaaat tagtaaaaga gtagtttacg tatgtccaga
     2101 atcaataagt tcgtacttac agtcagtctg ctgattttta tcatgatttc agcagttgcc
     2161 tgcgggatct acactcaaat ggtaaaggaa cgggtgtata gcctgaaaca gtccgttatt
     2221 gatactgctt ttgcggtggc aaatattgct gaatatcggc gtagcgtggc aattgatctt
     2281 atcaacacgc taaatcccac ggaggaacag ctgttggttg gcttacgcac agcttacgcc
     2341 gactcggttt caccctctta tttgtacgat gtcggtcctt atctgatttc cagtgacgaa
     2401 tgtattcagg taaaggagtt cgagaaaaat tattgtgcag atattatgca ggttgtgaag
     2461 tatcgacatg tcaaaaatac agggtttatc tcttttgacg gtaaaacctt cgtctattac
     2521 ctctatccgg taactcacaa tcgtagtctg atatttttgc ttggtctgga gcgtttttct
     2581 ttactgtcaa aatcgctggc gatggacagc gagaacctga tgttctctct gtttaagaac
     2641 ggtaaaccgg tgaccggtga tgaatataat gctaaaaacg ccatcttcac cgtttcggaa
     2701 gcgatggagc acttcgccta tttgccgacc ggattgtacg tatttgcgta taaaaaagat
     2761 gtttatttgc gggtctgtac attgattatt ttctttgccg cattggtggc agtgatatcg
     2821 ggtgccagtt gcctctatct ggtacgcaga gtgattaatc gtggtattgt ggagaaagaa
     2881 gccattatta ataaccattt tgaacgcgta ctggatggcg ggcttttctt ttcggctgcc
     2941 gatgtcaaaa aactctacag tatgtataac tcggcgttcc tggacgacct gaccaaagca
     3001 atgggtagaa aatcctttga cgaagattta aaagcgctgc cggaaaaagg cggttatttg
     3061 tgcctgtttg acgtcgataa attcaaaaat attaacgaca ccttcggtca tttgctgggc
     3121 gatgaagtgt tgatgaaagt ggtgaaaatc cttaaatcac agatcccggt agataaaggt
     3181 aaagtctacc gcttcggcgg tgacgaattt gcggtgattt atactggcgg cacgctggaa
     3241 gagttactat cgattctaaa agaaatcgtt catttccagg tgggaagcat taatttaagc
     3301 accagtatcg gtgtagcgca ttcaaatgaa tgtactaccg tcgaacgctt gaaaatgctg
     3361 gcggatgagc ggctgtataa gagtaagaaa aacggcaggg ctcagattag ctggcagtag
     3421 aattagaata gtgttagtac ccctgaatca aggggtacta atgaatgtgg taaatcattt
     3481 gcaaacattt tactttgcaa aaacttccgg cccttttatt gccaatgtat tcaaaatacg
     3541 tagtgccggg ccgctcggtt ttatttcatt acgttcccat ttcgagacac tttcttttga
     3601 cattcccatt gtatgtgcca gcacggattg tgacatgccg tatcgggtgc ggatggcttt
     3661 gatctcttca cccgtcattg atttgacttt ggctatacgg gcgctgactt cacgggcttt
     3721 gaccatgacg gcaatggcat caacctcact atcagacatc aggcccagtg cattcagttc
     3781 ctttgcatct tccagcatca tttgtaattt atcgctcatt tttcacctca atcaagaagc
     3841 cagtttcgat caaatcctct atttgcttat tgctaaaggc cagaaatgca tttgccattt
     3901 ttttgtaggc tgccagttca tcatcttcga tttctttgct tcctttactg cttagaccac
     3961 tcttggacca accatcataa aaaaacacat gatttgctga tttaaaaaag ataattgttc
     4021 tggctccacc actctttcct tgttggaggg gtaaccgttt tttgatgaca ccgctaccta
     4081 aatcagcctc ccatatgcct tgcatcactt cttcagcggc agccatgaga actttatcgt
     4141 gaataccaag ttttttcgtg ctttggctga atcgccttgc cttatacaca cccatacatc
     4201 catccttgtg gtaatgttta ctatagtaac cattacgctt atttcaagta attgcgatca
     4261 aaactttaat ttggtgtgaa aaagtgagag ttgcctcgag tacgattcat ttttctcgat
     4321 ttatgcgtca ggggattttg tgcgtaaggg taggacatcg ggaagggtag tagaggcaga
     4381 tcgaagaaca atatccttgt tagatctgcc tttagattaa ttaaacccgg ctaccccaca
     4441 aatcgtactc atcggcgtgc tcgactttca cacgcaggat atcacccggc ttaacgttgg
     4501 tttcaccatt gagataaacc gcaccgtcga tttccggtgc atctgccatg ctgcgaccaa
     4561 tcgcgccttc ttcgtccact tcgtcgataa tcaccagaat ttcacggccc actttctctt
     4621 gcaggcgctc ggcggaaatc tgctgctgca actgcatgaa acggttccag cgttcttctt
     4681 tcacttcttc cggaacctgg tcaggcaggg cattggcgtc tgcaccttca accgggctgt
     4741 atttaaagca gccaacgcga tccagacgcg cttctttcag gaagtcgagt agcatctgga
     4801 aatcttcttc cgtctcgcca gggaagccga caataaaggt tgagcgtagg gtcagttccg
     4861 ggcagatttc gcgccactgt ttgatgcgcg ccagttggcg atctacagaa cccggacgct
     4921 tcatcagttt gagaatgcgc gggctggcgt gctgcaacgg aatgtccaga tacggcagga
     4981 ttttgccttc tgccatcagt gggatgacgt cgtccacatg cggataaggg taaacgtagt
     5041 gcagacgtgt ccagatcccc agtttcgata actgttcgca caggctgacc atgctggttt
     5101 ttaccggctc gccgttgtgg aagccagtac gatgtttaac atcaacgcca taggcggaag
     5161 tatcctgcga gatcaccaga atctctttaa cgcccgcatc taccagacgt ttcgcttcac
     5221 ttaatacttc gccaatcgga cggctcacca ggtcgccgcg catagacgga ataatgcaga
     5281 aggtgcagcg gtgattacag ccttcagaaa ttttcagata ggcataatga cgcggcgtca
     5341 gtttgacacc ttgttctggc accaggctca ggaatgggtt gtgtttcggt tttggcacgt
     5401 agtgatgaac gtgctccaga acctgctcat agctatgagg cccggtgatt tccagcactt
     5461 tcgggtggac ttcgcggatc tgatcttctt ttgcccccag acaaccggtc acaattacct
     5521 tgccgttttc attcaacgct tcaccaatgg cttccagtga ttcttgtacc gcgctgtcaa
     5581 taaagccgca ggtgttgacg atcaccatgt ccgcatcgtc atagctcggt accacgtcat
     5641 aaccttcagt gcggagttcg gtgagaatac gctctgaatc gacaaggttt ttcggacagc
     5701 caagggaaac aaagccgatt ttcggctggg gagttacttt gctcatagct taaaaaatat
     5761 tcagttacag gaaaggtcag ggcagggatt ctacagagtt ctggataaaa tttgtatcgc
     5821 aatctcattc gctggcggag gcgaaggaaa tgtaaatttt gttaattcgg cgtgaagaat
     5881 tgatcctgga cagcattatg ctcaaaaaat agccatacta tttaattgca acaaggctgg
     5941 aaagaggagg atcgaagtat gttcgttgac agacagcgaa tcgatctgct gaaccggttg
     6001 atcgacgcac gcgttgacct cgccgcatac gtgcaactga ggaaggcaaa aggatacatg
     6061 tccgtcagcg aaagcaatca tctacgagat aactttttta aactgaatcg cgaactgcac
     6121 gataaatcgc tgcggttgaa tcttcatctg gatcaggaag agtggagtgc tcttcatcat
     6181 gctgaagaag cattagcgac agccgcagta tgtttgatga gtgggcacca tgattgcccg
     6241 actgttatta ccgtcaacgc cgataagctt gaaaattgtc tgatgagctt aacgctgagt
     6301 atccagagcc tgcagaagca cgccatgctt gagaaggcct gaaaactaag ggggagaaag
     6361 cgtctccccc ttcatgttta agtttttgta aaaatgaatt tgttatctcc tccactgact
     6421 acgctttaag ccagagtcaa tccggaggcg ttatgcatcg acaattcttt ttccttgtgc
     6481 cccttatttg tctttcttcc gctctctggg cggctcctgc aacggtaaat gtcgaagtac
     6541 tgcaagacaa actcgaccat ccctgggcac tggccttttt acccgataac cacggtatgt
     6601 taatcactct gcgcggcggc gagttgcgtc actggcaagc aggaaaagga ttatctgcgc
     6661 cgctttccgg agttccggac gtttgggcgc acgggcaggg cggcctgctg gacgtggttt
     6721 tagcgcctga ttttgctcag tctcgccgca tctggttaag ttattccgaa gttggcgatg
     6781 atggcaaagc cggaactgct gtggggtatg gccgcttaag tgatgatctc tcaaaagtga
     6841 ccgactttcg caccgtcttt cgccagatgc caaaactgtc taccggcaac cattttggcg
     6901 ggcggctggt attcgacggt aaaggttatc tttttattgc tctgggcgaa aacaatcagc
     6961 gcccgacggc gcaggatctg gataaattac agggcaaact ggtgcgtctg accgaccagg
     7021 gcgaaatccc ggatgataat ccttttataa aggaatccgg tgcgcgcgcc gagatctggt
     7081 cttatggcat tcgtaatccg caaggaatgg cgatgaatcc gtggagtaat gcactgtggc
     7141 tgaatgaaca tggcccgcgc ggtggtgatg aaattaatat cccgcaaaaa ggcaaaaact
     7201 acggctggcc gctggcaacc tggggaatca actattcagg ctttaagata ccggaagcga
     7261 aaggggagat cgtcgccggg accgagcaac ctgtttttta ctggaaagat tcgcccgccg
     7321 tgagcggcat ggccttctat aacagcgata aattccccca gtggcagcaa aaattattta
     7381 ttggcgcgct gaaagataaa gatgtcattg tgatgagcgt caacggcgac aaagtaacag
     7441 aagatggccg tattttaacg gacagagggc agcgaattcg tgatgttcgc actggacccg
     7501 acggttattt atacgttctc accgacgagt ccagtgggga attacttaaa gttagcccac
     7561 gcaattagct aacgggaatc atcaccactt tgcgcactgc ggggcgttca gtgagttgct
     7621 gataccagcg ttgcagattt ggacgcggtg tccaggtcag gccaacatta aacaaattgt
     7681 agatgaacgg tgcgatagcg atatcgccca caccaaactc gtcgccagag aaccatttta
     7741 cttttgccag ttccgcatcg agcagggcaa acagggcgtc gcactctttg caactggcat
     7801 caatggcggc ctgatcgcgc tcttccggtg gtgttctgac taatcccatc aggatcccgc
     7861 gatgagcatt actgagtgtc tggtttgccc agtccatcca tttttctgct tccgcacgac
     7921 gtgccggtga gtcgatccac aggcgttttt gcccgtactg tgccgccaga tagcggacaa
     7981 tggcgtttga ttcccaaaga atgagatcac tttcgtcgtc acgcaacaac ggcaccagcc
     8041 cgttagggtt catcgccaga aaatcagcat cgtgatttat cccaaactca cggcccgcga
     8101 gaatttgctc ataaggtagt tccagttctt cgagcgtcag caatactttt tttacgttgg
     8161 ttgaattatt ccgaccccac agcgtaatca tactcacccc ttttccgaca ggcagccaca
     8221 ggctgaacag gactccgatg gtgaagtaaa gttaactttt aagcaacagc tggcaaaaaa
     8281 atgacaccag aagcagcgaa gcggcaggat attgcataaa ctttaaaaac tttaccaact
     8341 tacggtttct ttaagtttgt gtgtgcgtta ttaatcaccg aacttatcat acggcgatat
     8401 aacgtatttt tttgaatgga tactcgggtg gcatttatga cgcaatactc ctctctcctt
     8461 cgtggtcttg cagcgggttc tgcattttta ttcctttttg ccccaacggc attcgcggcc
     8521 gaacaaaccg ttgaagcgcc gagcgtggat gcgcgtgcat ggattttaat ggattacgcc
     8581 agcggtaaag tgctggcaga aggcaatgcg gatgagaaac tggatcccgc gagcctgact
     8641 aaaatcatga ccagctatgt ggttgggcag gcgcttaagg ccgataagat taaactcacc
     8701 gatatggtga cggtcggtaa agatgcctgg gcgacgggaa atccggcact gcgtggttca
     8761 tcggtaatgt tcctcaaacc gggcgatcag gtttcggtgg cagacttgaa caaaggtgtg
     8821 attatccagt ccggtaatga cgcctgtatt gcgctggccg attacgttgc cgggagccag
     8881 gagtcattta ttggtttgat gaatggttat gccaaaaaac tggggctgac caacactacc
     8941 ttccagacgg tgcacggtct ggatgcgccg gggcagttca gtaccgcgcg cgatatggca
     9001 ttgctgggta aagcattgat ccacgatgtg ccggaagagt acgccattca taaagagaaa
     9061 gagttcacct tcaacaaaat tcgtcagcct aaccgtaacc gtctgctgtg gagcagcaat
     9121 ctgaatgttg atggcatgaa gacaggaacc acggcaggcg cgggatataa tctggttgct
     9181 tcggctaccc agggcgatat gcgtttaatc tccgtagtgc tgggggcgaa aaccgaccgt
     9241 atccgtttta atgagtctga gaaattattg acctggggtt tccgcttctt tgaaaccgtg
     9301 acgccaatta aacctgatgc cacctttgtg actcagcgcg tctggtttgg tgataagagc
     9361 gaagtgaatc tcggggcagg cgaagcgggc tcagtgacca taccgcgtgg gcagctgaaa
     9421 aacctgaagg cgagctacac gttaacggaa ccgcagctta ccgcaccgct gaaaaaaggt
     9481 caggttgtcg ggaccattga tttccagctt aacggtaaat ccattgagca gcgtccgctg
     9541 atcgtgatgg aaaatgtgga agagggcgga ttctttggtc ggatgtggga tttcgtgatg
     9601 atgaaattcc atcagtggtt cggcagctgg ttctcttaat cttctgataa ccggatggcg
     9661 cgaaacgtca tccggttata cgtcattaat acatcaactt aatacgctgc gtctgcgcgt
     9721 acttcacata ctcatcttcc gggcaacaat cgctcaccac aatatcaaag cgtttcaggt
     9781 cacccatgcg cgccggacgc accttgccaa atttactgtg gtcaacaacc agcacatgct
     9841 tttgcgccat cgacatggcc cagtgtttta ccggcaactc ttcaagatta aaacaggtag
     9901 cgcctttact gacatgcacg cccgccgcag aataaaaagc gatatccggg caaaaattat
     9961 tcagcgtttg ctgaaaatcg atgggtttga aaatggcgtt gctggcgtga aattcaccac
    10021 cgcacagaaa tgcgcggcaa tggggttttt ctttcagcgc cagaaaggta tttagcgaat
    10081 aacaaacggc ggtaaaaggg atttcattat caatcgcttc aataatccac ggcgtggtgg
    10141 tgccacagtc aaaaaagagg gtctgatcgg gttctaccag tgtcgcagcc agttttgccg
    10201 cccggcgttt ttcttccacc aggcgggatt tttgatcgct taacaggtaa tggctggcgc
    10261 tgcgcggttc cagaacaata tagccgccga gcaaaacgac gggcgcactg tggttgttca
    10321 gatcgcgacg aatcgtcatc tccgaaaccc caagcagggc ggcggcgtct ttaagatgta
    10381 acttatcgct gcgttttaat tcttgcagca gctgcccgat acgctcttcg cgacgtgttt
    10441 ccataatccc tctgaatagt tattgaagcg agccgctcaa tactacactt tttagcagag
    10501 atcagtcacg cacccagcct ttgcggatcg gtaatgcaaa acagaagcga taccacgatt
    10561 gcagacgttg atagagttta tgccccatcg cttgccagat aatctgggca ctaaggcagc
    10621 caatcatacc tgccagcaat ccaccgagca tatccagcgg ccagtggacg ccaagataaa
    10681 cgcgcgacca ggcaatgacg acggccagca ccattaaaag tgagccggac cacaggcgat
    10741 gccagcataa aaatgccagt gcaaaggtga aaatcaccgt accgtgatcg ctcgggaatg
    10801 agtcatccgc cgcatgatgc aggaagttat agccgatatt ttcgacaaac ggtcggtcgt
    10861 gcggaaaaag atgtcccatc gtccaggaca ccaacaggct gaccgccagc gcgatagcga
    10921 tttttatcac cagttgccgt tgtgctgtaa gcccccacaa ccaaagtacc gcggccagca
    10981 gcggcaccac ggtaatcaaa tctttagcaa taaaaatcgc caacgagatc atccacggag
    11041 ccgagtctgg cgtcgcgtta ataagagaga atagagagag attcaaattt tccagcataa
    11101 cttcccgacg caaagtgatt aaaagggagc caatacaggc aagtcgttga gaataaagtg
    11161 caggttaaac tgggtaaagc ggcatcgtct tatttccctc aagcggcctg tttacggtgg
    11221 gtgattgtaa cgggcatagg ttaaataaaa cttaaagaaa gcgtagctat actcgcaata
    11281 atgtaagaat gtgcttaacc gtggtttcag ctacaaaatt cgctttctcg ttagctgcgc
    11341 ttttattaaa ctctgcgcga ttattattgg cgaagaaatt gcatgcaaaa taaattagct
    11401 tccggtgcca ggcttggacg tcaggcgtta cttttccctc tctgtctggt gctttacgaa
    11461 ttttcaacct atatcggcaa cgatatgatt caacccggta tgttggccgt ggtggaacaa
    11521 tatcaggcgg gcattgattg ggttcctact tcgatgaccg cgtatctggc gggcgggatg
    11581 tttttacaat ggctcctggg gccgttgtcg gatcgtattg gtcgccgtcc ggtgatgctg
    11641 gcgggtgtgg tgtggtttat tgtcacctgt ctggcaatat tactggcgca aaacattgaa
    11701 caattcaccc tgttgcgctt cttgcagggc ataagcctct gtttcattgg cgctgtggga
    11761 tacgccgcaa ttcaggaatc cttcgaagag gcggtttgta tcaagatcac cgcgctgatg
    11821 gcgaacgtgg cgctgattgc tccgctactt ggtccactgg tgggcgcggc gtggatccat
    11881 gtgctgccct gggaagggat gtttgtcttg tttgccgcat tggcagcgat ctcctttttc
    11941 ggtctgcaac gagccatgcc tgaaaccgcc acgcgtatag gcgagaaact gtcgctgaaa
    12001 gaactcggtc gtgactataa gctggtgctg aagaacggtc gctttgtggc gggggcgctg
    12061 gcgctgggat tcgttagcct gccgttgctg gcgtggatcg ctcagtcgcc gattatcatc
    12121 attaccggcg agcagttgag cagctatgaa tatggtttgc tgcaagtgcc tattttcggg
    12181 gcgttaattg cgggtaactt gctgttagcg cgtctgacct cgcgccgcac cgtacgttcg
    12241 ctgattatta tgggcggctg gccgattatg attggtctgt tggtcgctgc tgcggcaacg
    12301 gttatctcat cgcacgcgta tttatggatg accgccgggt taagtattta tgctttcggt
    12361 attggtctgg cgaatgcggg actggtgcga ttaaccctgt ttgccagcga tatgagtaaa
    12421 ggtacggttt ctgcggcgat gggaatgctg caaatgctga tctttaccgt cggtattgaa
    12481 atcagcaaac atgcctggct gaacgggggc aacggactgt ttaatctctt caaccttgtc
    12541 aacggaattt tgtggctgtc gctgatggtt atctttttaa aagataaaca gatgggaaat
    12601 tctcacgaag ggtaaaaaaa tgcctgattg ctttgtgcga tcaggcattc tcgaattaat
    12661 ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc acctacgctg gcaagatggc
    12721 gaacataagg atgcgggtgg taagccggag ctggcgcagg agccacatag acggtttgcg
    12781 gcacagtcgc gacgctgacc gcttgtggaa cgctgactga atcaggaacc acaaccactt
    12841 tgtatccact cggcacatcg acggtgacac tttgcgccat cgccacacca gtaaagccca
    12901 ttaaaagcgc gcccaacagt agacaatttt tcataatcat ttcgcctgaa ataatgcagc
    12961 gggttaattt ccgcgaatta tgcagccttc attcaggtat tgtgttgccg ggcgtgctgc
    13021 cgtttttatt ggtcaaatgg cgcttcgtgt ttaagaactt tatcgatcac atccagtacg
    13081 ccttcacggt tattggagcc tgcccggtat tttgccgctg cgacgaccgc gttgccggca
    13141 ttttccattg caaaactaaa gcctgcctga cgcagcatct caatatcgtt accgccatcg
    13201 ccaaagacca ccacttcgct gtcgtctatt ccccataatt tctgcagttg gcgaaggcca
    13261 ttggctttat gtacgccggg gataatcaga tcgatgctgc cgttgccggt gtggaccggc
    13321 accataatat cgccgatggc ctcatgtaat gttttttgta cttgtggaat cagttcatcg
    13381 gaaagattca ggccaaactt aaagaagata tcttctaagt tgtcaaagtt atcgacgtat
    13441 tccagacggt gataatacat ttccgccacc attttcatgg catcgtcata ttttttgagc
    13501 gtataggcgc tattttttcc gcaggcaata atttccactt ccgggcgcgt cagcaaatgt
    13561 tccacgacag tagtaaacgc atccttcgac agctcgccat taaaaacatc tttgccttca
    13621 ctcactaccc agccgccgtt ttcagccaca aaggcaattt cattagcaat ttcagggaag
    13681 aaagagatca actgataata ttgattcccg ctggcgacca caaagcgaat gccttgtgct
    13741 ttcatttgct gatactgagc cataaactgc tcacggttat aggttttttg atcgcttaag
    13801 aaagtaccat ccatgtctac cgcaattaat ttaatgctca tcaactattc tccatcgccg
    13861 tctgcgtttt ggtatcgggt ttggcgacgg ctttcgcgac aatagccgcg agaataacca
    13921 gcgccagtac aaccagcatt gcactacgta atccataatg ttcgccgaga tagcccagca
    13981 gcggcggccc gacgaggaaa gccagataac cggtcgttgc taccacactg acgcgggttg
    14041 gtgcatcggg gccggtatcg ctggcggcag aaatggtcag cgggaagccc agcgaggcac
    14101 ccagtcccca gagtacaaca gacaccccag cgacccaggc gctatcgaca aaaataatca
    14161 gcccaatacc caacgccccc attagcgcac tggcccgaac cacggcaacg cgactgtaac
    14221 ggtcgatgaa ccaaccgcca gtgaagcgtc caacggtcat ccccagggta aaaccggcat
    14281 aaatcagcga gccggaagtg gggctaaaac cgtgaccgtc aaccattaat aagggtaacc
    14341 agtcgttggc agaaccttcg gcaaaggcca tcgccagcac cacaacacca atcagcagca
    14401 actggatatc gcgataaaaa ggtacgcctt tttcgccatg ctgggtgcca tcggcagcat
    14461 ttttgcccgt accgtcaggg attgcctgaa tggcgatata tataggcgcg atgcctacca
    14521 gcgccgccaa taaaatgtgc accgttgccg gaacgccaaa ggccgtcagt gccatcccga
    14581 caccagcgcc tgccagcgtg cccaggctat aaaaaccgtg catcatcggc aaaaccgttt
    14641 tattcatttc tcgctcaacg gcggcacctt caacgtttat cgccacttcc gcagaaccaa
    14701 aacttgcccc aaagacgccg agaccaacgg caaagagcag gggcgatgtc agccagagtg
    14761 ccagacttaa tatcatcatc ccgaccaatg cgcaggacat ggtgaccagg atgacattac
    14821 gtgtcccaaa gcgtttcact aaccacgccg agcagagaat accgctcatc gaaccgatcg
    14881 acagaccaaa gagaacaccg cccatttcag cgatcgagac agagagaata tcgcggatag
    14941 caggcgtacg ggttgcccag gacgccatta acaggcccgg caaaaagaag aacataaaca
    15001 gcgcccaggt tcggcgtttc aatgcattac gtgaagaatt tacggtcata gatcacgtca
    15061 aaataagaag agggaagaca acattagcaa ggttgtgtac atttgtacac aattgcagac
    15121 agaggaaatg acatgcgtcg cgctaacgat ccgcaacggc gagaaaaaat tatccaggcc
    15181 acgctggagg cggtgaaact ttacggaata catgctgtta cgcaccgcaa aattgctacc
    15241 cttgccgggg taccgttggg atcgatgacc tactattttt caggaattga tgagttgttg
    15301 ctggaggcgt tcagcagttt tactgagatc atgtcccggc aatatcaggc attttttagc
    15361 gatgttagtg atgctcaagg cgcatgccag gctatcaccg atatgatcta cagctcacag
    15421 gttgcaacgc cggataacat ggagctgatg taccagctct acgcgctggc tagccgaaaa
    15481 ccgctattaa aaacggtaat gcaaaactgg atgcagcgca gtcagcaaac gctcgaacaa
    15541 tggtttgaac ccggaaccgc ccgcgcgctt gatgcgttta ttgaggggat gacgctgcat
    15601 tttgtcaccg accgtaagcc gctatcgcgc gaggagattt tgaggatggt tgagagggtt
    15661 gcagggtagt agataaattt caggcaacaa aaaacccatc aaccttgaac cgaaatggcg
    15721 gggttgatgg gctccacaaa atggggacat caaagaaaag cagtggcact aattaagact
    15781 gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag ggcaacttca
    15841 gttttatcct aatcctggcc ataccatgac gatgattgtc cctgccagcg tcagcaggac
    15901 gttggcgatt gcgtaggtgc ccgcatagcc cagcgccggg atgttactgc gagctgtatc
    15961 actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag
    16021 tgcgcggttc attcgcaata cataagcacc gaacaagaaa cagataacca cgggcacaag
    16081 actgacaatc aatccggcaa tcaacatctg accgccaatc gcgcccagac cgttattaat
    16141 accgctaccg gcgctcagac caacgcctgc cataaacacc atcaagccga actctttcac
    16201 catgcttaat gccccttgcg gaatgtaacc gaaggtcggg tggttagcac gcataaagcc
    16261 cagcataatt ccggcgaata acaacccggc agcgttcccc atgccgaaac tgaatgtgct
    16321 gaactggaag gtgatcatcc cgatcatcag cccaataaca aagaaggcgc agaatgccag
    16381 caggtcagtg acctggctgt gaatcgagat aaagccgatg cgatcggcga tggtttttac
    16441 gcggcgggca tcgccgctga cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc
    16501 aatcggcatc tcaatctggc tacgaatgac gcggttaagg aagcaaccgt gatcggtcaa
    16561 cttcagttgt gcgagacgtt tacctacagc gttatggttt ttaacgacca cttcttcagt
    16621 gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac ggaagctggg
    16681 atcgagtcgg gcatgggcgt cgggatagcc taccaacgct atttcatcgc ccatttgtag
    16741 cacggcatca ccgtctggat ttgccagaat cccgttacgt cgaatacgtt caatgtagca
    16801 gccggtttgt cgataaatac ccagttcacg cagatttttg ccgtcggtcc aggccaccag
    16861 ctccgggccg acgcgatagg cgcggatcac cggtaaataa accttacggt tggcatcagt
    16921 gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa
    16981 tttcggcaag taacgcgcac caacaatcaa actcaccaga ccgattaaat aggttaaggc
    17041 atacccgagg ctcagattat ccagtgccag tgagagctgc ctgctttcca taccggaatg
    17101 acgcagtgta tcgccagcac cgaccagaac cggtgtcgac gtcatagagc ctgctaacat
    17161 accggccgtc aggccaatat cccagccaaa cagcttacct aaccctaagg cgatcaccag
    17221 cgcactgcca accatcacca gtgctaacat taggtaattt ttcccatcgc gaaaaaaaat
    17281 ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa ataaacagca taaagccaag
    17341 attaagcgca tcggtgttaa tgctgaaatg ttgttggcct aataacagcg atacgactaa
    17401 aacgccaatg gaattaccca gttggatcga accaagtcgt aactttccga gacatagccc
    17461 aagcgcgagg accacaaata ataacagaat gtaattccca tttaacaatt cggcgacgtt
    17521 tatattcacg gaggctaact tcttgtttac tagtaagctg ttgaaagaaa tggtaattta
    17581 tgataatgtt ttttaccaga attcagggcg cagattcatt cagcgcacct aaacgatagt
    17641 aaagtaacaa tatattttac tagtgtaatc acattaggta tcaacggcta tatgaattgc
    17701 gttggcctat attagcatgg aatgcgaagc ggctttatct tactgaacgc cacactggcg
    17761 aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt gaaacataaa caacgttggg
    17821 cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg gcgacgcaca
    17881 tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt ttcattcttc
    17941 ctggagcagt tgccagtttc ttttcacagc gtagagaagt cctgaaacct ctatttggcg
    18001 caatgctggc ggcaccctgt tcaatgctta ttatgcggct gtttttttca ccgacccgct
    18061 cattctggca agagctggca tggttactaa gcgcggtgtt ctggtgtgcg ctgggggcac
    18121 tgtgtttctt atttatcagt agcttgttta aaccacagca cagaaaaaat cagtaaagcc
    18181 ctcaacgcga gggcttgtca gacgatcagg cgtccagatt ctctttcacc catgcagcaa
    18241 aatcggtata gccgccgata tgttgctgat cgacaaaaat ctgcggcacg gtttctacgg
    18301 gtttacctgc cttttgttgt agatcttctt tagtgatccc ttccgcacga atatctacat
    18361 actgatactg aaaatcatcg cgttcattgc tcaatttctc agccagatct tttgcacgca
    18421 cacagtaagg gcaacccgga cgaccaaaaa taacggtttg cattatttct ctcctcatag
    18481 atttatgcct gtaatgatca cgctaaaatg tattcgctga aagtaggttt aacctgttgc
    18541 attaattgct aaaagctata actgttaaac acaatacagt gaaaagtttt agactgaagg
    18601 ttcactttgc agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaaa gcgtgttgat
    18661 actggaattt atcggaatga tgttgctggc ggtggcgctg ctgtcggtaa gcgactccct
    18721 gtcgctgcct gagccatttt ctcggccaga agtgcagatt ctgatgattt ttctcggtgt
    18781 tttgctcatg cttcccgctg cggtggtggt tattcttcag gtggcaaaac gtcttgcccc
    18841 acagctgatg aaccgtccac cgcagtattc acgttcagaa agagaaaaag ataatgacgc
    18901 caaccattga acttacttgt ggccatcgct ccattcgcca tttcactgat gaacccattt
    18961 ccgaggcgca gcgtgaggcg attattaaca gcgcccgtgc gacgtccagt tccagttttt
    19021 tgcagtgcag tagcattatt cgcattaccg acaaagcgtt acgtgaagaa ctggtgacgc
    19081 tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc tgtgccgact
    19141 ttaaccgcca tttacagatc tgtccggatg ctcagcttgg cctggcggaa caattgctgc
    19201 tcggtgtcgt tgatacggca atgatggcgc agaatgcatt aaccgcagcg gaatcgctgg
    19261 gattgggcgg ggtatatatc ggcggcctgc gcaataatat tgaagcggtg acgaaactgc
    19321 tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg cctgcggata
    19381 atccggatct taagccgcgt ttaccgtcct ccattttggt gcatgaaaac agctatcaac
    19441 cgctggataa agacgcactg gcgcagtatg acgaacaact ggctgaatat tacctcaccc
    19501 gtggcagcaa taatcgccgg gatacctgga gcgatcatat ccgccgaaca atcattaaag
    19561 aaagccgccc atttattctg gattatttgc acaaacaggg ttgggcgacg cgctaaaacc
    19621 gccacgccga tgtatgatac gcgggctttt gaccaggtct gacagagagg tgcagggtga
    19681 aaattgccat attgtcccgg gatggaacgc tctattcgtg taagcggctg cgtgaagccg
    19741 ctatacagcg cggtcacctg gttgaaattc ttgatccgct ttcttgctac atgaacataa
    19801 atcctgcggc gtcttctatt cactacaaag gccgcaagtt accccatttt gacgcagtga
    19861 tcccgcgtat tggcaccgcc attacctttt atgggacggc ggcactgcgc cagttcgaga
    19921 tgctggggag ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt gacaaattgc
    19981 gttccatgca actgctggcg cgtcagggca tcgacctgcc tgtcacgggc attgcgcatt
    20041 cgccggatga taccagcgat ttaatcgaca tggtcggtgg tgcgccgctg gtggtcaagt
    20101 tggttgaagg cacgcaggga attggcgtcg tgctggcgga gacgcgtcag gcggcggaaa
    20161 gcgtgattga cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa tatatcaaag
    20221 aggcgcaagg gtgcgatatc cgctgtctgg ttgttggcga tgaagtggtc gctgcgattg
    20281 aacggcgggc gaaagagggc gattttcgtt ccaatttgca tcgtggcggc gcggcaagcg
    20341 tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga acgatggcgc
    20401 tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg atggaagtga
    20461 atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg tatcgacatc gcgggtaaaa
    20521 tgatccgctg gatcgaacgc cacgctacga cagaatattg cctgaaaacg ggtggttagt
    20581 cgcaatcaca ttactgatca tggttttgcc tgctcttttt gcgtaagctg tgccggtctt
    20641 tttatcgaaa gaggttgtac aaaattatga catcgctggt cgttcctggt ctggatacgc
    20701 tgcgtcaatg gctcgatgac ctggggatga gtttttttga atgtgataac tgtcaggctc
    20761 tgcatctgcc ccatatgcag aatttcgacg gtgtctttga tgccaaaatc gatctgatcg
    20821 ataacacgat cctgttttct gccatggcgg aagtccgacc ttcagccgta ttgccgctgg
    20881 cggcggattt atctgccatc aatgccagtt cgctgaccgt gaaagcattt cttgatatgc
    20941 aggatgataa tctgccaaag ctggtggttt gccagtcttt atccgttatg cagggcgtaa
    21001 cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg atggtcattc
    21061 ttgaagctaa tgccatcaac tgctgttacc gactgacgat gaagggcaaa acaacgttac
    21121 cgaaaactat ttcctccact gataactcct ttcgagcacg cagtcgctgg tgcagtggct
    21181 gcgcgctgca aaattatctg ctgtttttaa ccttttctta aagattattt cacttctctt
    21241 gtgtcgattt ggctttatca catagagcaa atacgcataa aaatttgtta aataccgttt
    21301 tttaatccga gctatagtct caaaccctgg ctaaagttat tcttgcgatg cttttatata
    21361 gcgagcagtg ctggccggga gagagttctc ttttcttaca ccgcgccgat aaaaaatatg
    21421 cacgtttatt gcatatcttt cagtgtgaca acttttgttc gtttgttaac gaactttcag
    21481 aaggaaagag atatgaccgc cttaaataaa aaatggcttt cgggtctggt tgcgggtgct
    21541 ctgatggccg tctctgtcgg cacgctcgcg gctgaacaaa aaacactcca catttataac
    21601 tggtctgatt atatcgcccc cgacacggtg gctaactttg aaaaagaaac cggtattaaa
    21661 gtcgtctacg atgttttcga ctctaacgaa gtactggaag gcaaattaat ggccgggagc
    21721 accggctttg acctggtggt gccgtcagca agctttcttg aacgccagct gacagcggga
    21781 gttttccagc cgctggataa aagcaaattg ccggagtgga agaatctcga cccggaactg
    21841 ctgaagttgg tcgccaaaca cgatcccgac aataaatttg ctatgcccta tatgtgggcg
    21901 acgaccggga ttggctataa cgtcgataaa gtcaaagcgg tgctgggcga aaacgcgccc
    21961 gtcgatagct gggacttgat cctcaagcct gaaaatctgg aaaaactgaa aagctgcggt
    22021 gtatctttcc tcgatgcgcc agaagaagtt tttgctaccg tgctgaatta tctcggcaaa
    22081 gatcccaaca gcactaaagc ggatgattac accggaccgg ctaccgatct gctgttaaag
    22141 ctgcgcccga acattcgtta tttccattca tctcaatata ttaacgacct ggcaaacggc
    22201 gatatttgcg tcgctatcgg ctgggcaggt gatgtctggc aggcgtcaaa ccgcgcgaag
    22261 gaagcgaaga atggcgtgaa tgtctcgttc tcgattccaa aagaaggggc gatggcgttc
    22321 tttgatgtat tcgccatgcc tgcggatgcc aaaaataaag acgaagccta tcagttcctg
    22381 aattacctgc tgcgtcctga tgtggtggca catatctctg accatgtgtt ctatgctaac
    22441 gccaataaag cagccacgcc gctggtgagt gcggaagtcc gtgataaccc gggtatttat
    22501 ccgcctgcgg atgtccgcgc gaagttgttc actctgaaag tacaggctcc gaaaatcgac
    22561 cgtgtgcgca cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc agccttagat
    22621 gccgggcggg agcgccactc cctccggcaa ttcgcaccaa catggtgcgc ttgcacacat
    22681 ttaatgccgg agagcagccg tgaatgacgc tatccctcgc ccccaggcga aaacccgtaa
    22741 ggcgctgacc ccgctattag aaatccgcaa tctgaccaaa tcctacgatg gtcagcatgc
    22801 ggttgatgat gtcagcctga ccatctacaa aggtgaaatc tttgcgctgc tgggcgcatc
    22861 cggctgtggc aagtccacgc tgctgcgtat gctggcaggt ttcgaacaac cttcagccgg
    22921 gcagattatg cttgatggcg tcgatttgtc acaggtgccg ccttacctgc gccccatcaa
    22981 tatgatgttt cagtcttacg cgctgtttcc gcatatgacc gtggaacaga acatcgcttt
    23041 tggcctgaaa caggacaaac taccgaaagc ggaaattgcc agccgggtca atgagatgct
    23101 cggccttgtg catatgcagg agttcgccaa acgcaaaccg catcagcttt ccggtggtca
    23161 gcgacagcgt gtggcactgg cccgaagcct tgccaagcgc ccgaaactat tactgctcga
    23221 cgagccgatg ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga
    23281 tattctggaa cgcgtcggtg tgacttgtgt gatggtcacc cacgatcagg aagaggcgat
    23341 gaccatggcg gggcgtatcg ccattatgaa tcgtgggaaa tttgtccaga ttggcgaacc
    23401 ggaagagatc tacgagcatc cgactacccg ctatagcgcc gaatttattg gctcggttaa
    23461 tgtctttgaa ggtgtactca aagagcgtca ggaagatggt ctggtgcttt attcgccggg
    23521 gctggtgcat ccactgaaag tcgatgcgga tgcttcagtg gtcgataacg tgccggtaca
    23581 cgtagcgctg cgtccggaaa aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa
    23641 cttcgcggtg ggggaggtga tacacattgc ctatctcggc gatctttcgg tgtatcacgt
    23701 tcgtctgaaa agtgggcaga tgattagcgc ccagctacaa aacgcccatc gtcatcgtaa
    23761 agggttaccg acctggggcg acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt
    23821 gctgacggtt taaggagcaa agatgagtac acttgaacct gctgcccagt cgaaaccgcc
    23881 gggcggattt aagctgtggt tgtcgcagtt gcaaatgaag catgggcgca aactggtcat
    23941 tgcgttgccg tatatctggt tgatcttgct gtttctgctg ccatttctga ttgtctttaa
    24001 aataagcctg gcggagatgg cgcgcgctat tccaccttat accgaactga tggagtgggc
    24061 tgacgggcaa ctgtccatca cccttaattt cggtaatttc ctgcaactga ccggcgatcc
    24121 gctctatttc gatgcttatc tccagtcgtt acaggtggcg gcgatttcga cattttgctg
    24181 tttactgatc ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa
    24241 tatcttatta ctactggtga tcctgccgtc gtggacctcg tttctgattc gtgtctacgc
    24301 ctggatggga atattaaaaa acaacggtgt gctgaataat tttctgctgt ggctgggggt
    24361 tatcgatcaa ccgctgacca ttcttcatac caatctggca gtttatattg gcattgttta
    24421 cgcctacgtg ccgtttatgg tgctgccgat ttataccgcg ttgattcgta ttgattattc
    24481 gctggtggaa gcagcgctgg atctcggtgc acgaccgctg aaaacgttct ttactgtgat
    24541 cgtgccgctg actaaaggtg ggattattgc cggatcgatg ctggtgttta tcccggctgt
    24601 gggcgagttt gtgatcccgg aactgctcgg tggcccggac agcatcatga tcgggcgcgt
    24661 gctatggcaa gagtttttca ataaccgcga ctggccggtg gcctcggcgg tagcgatcat
    24721 catgttgctg ctgctaattg tgccgataat gtggtttcac aaacaccagc aaaaaagcgt
    24781 gggagaacac ggatgaataa tttaccggta gttcgttcgc cctggcggat tgtgattttg
    24841 ctgttgggct tcacctttct ctacgcgcca atgctgatgc tggttatcta ttcgtttaac
    24901 agctcgaagc tggtgacggt gtgggccggc tggtcaacgc gctggtatgg cgaattattg
    24961 cgcgatgacg cgatgatgag tgcggttggt ttaagtctga caattgcggc ctgtgcggcg
    25021 acggcggcgg cgatcctcgg aactattgcg gcggtggtgc tggtgcgctt tggcaggttt
    25081 cgcggatcaa atggctttgc ctttatgatc accgcgccgc tggtaatgcc agatgtcatc
    25141 acgggcttgt cgctgttgtt attattcgtc gcgcttgctc atgccattgg ctggcctgcg
    25201 gaccgcggta tgcttaccat ctggctggcg catgtcactt tctgtacggc ttatgtggca
    25261 gtcgttattt cgtcgcgtct tcgggaactg gatcgctcga tagaagaagc agcgatggat
    25321 ctcggtgcga cgccgctgaa agtgtttttc gtcattacgc taccgatgat catgcccgcg
    25381 gtcatttctg gctggttact ggcttttact ttgtcgcttg atgatctggt gatcgccagc
    25441 tttgtatccg ggccgggagc caccacgtta ccgatgctgg tcttttccag cgtgcggatg
    25501 ggggtgaatc cggaaatcaa cgccctagca acattgatcc tcggcgcggt cggaattgtc
    25561 ggatttatcg cctggtatct gatggctcgc gcagaaaaac agcggatacg cgacatccag
    25621 tgtgcaagac gtggctgaag acactaaaat ttgccaacct ggctacataa tgccgcgtat
    25681 gtcgcggcat tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa acatcttcat
    25741 ctcatgctcg cctgaatgtg cctgcgcttg tgcaggtggc ggcgctcgcc attattatga
    25801 tccgtggcct cgacgtgctg atgattttca atacgctggg cgtgcgcggt attggcgagt
    25861 tcattcatcg cagcgtacaa acctggagtt taacgctggt ctttttaagc agtctggtgc
    25921 tggttttcat tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct
    25981 ggctgtatct gctgacacaa atcactgccg caagttactt gtgggcggct tcgctggggt
    26041 acggttatcc ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc
    26101 tgatgctgca gaagctgccg gatatgctca tcctgatgct gctgttcgtt ccctcgacca
    26161 gtcggcggtt cttccagttg caataatgtg tataatcgtc gcccctgatg atgtgaaggt
    26221 caatgtatgc agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg
    26281 cagccgattc cagagcaact ctccgctaaa accgccgatc ttaaaaatct gctcgccgac
    26341 tttccggttg aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa
    26401 gccaaaatgg tggtgagtgg tagcgttgaa aaaccactgc tcggtatgct gcatcgcgac
    26461 ggcacaccgg aagacctttg tgactgcccg ctttatcctg cctcatttgc gcccgttttt
    26521 gcggcgctaa aaccgtttat cgcccgagcg gggttaacgc cctacaacgt ggcgcgtaag
    26581 cgtggcgaac tgaaatacat tctgctgact gaaagccaga gcgatggcgg catgatgctg
    26641 cgctttgtac tgcgttctga aaccaaacta gcacaactgc gtaaggcatt gccgtggtta
    26701 caggcacaat tgccgcagct aaaagttatc accgtcaata ttcagccggt acatatggcg
    26761 attatggaag gggagacgga gatctacctg accgaacaac aggcgctggt agagcgtttt
    26821 aacgatgtgc cgctgtggat ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc
    26881 agccagctgt acgctaccgc gcgcgactgg gtacgacagc tgccggttaa acatatgtgg
    26941 gatctgttct gcggtgtggg gggctttggt ttacactgcg cgacgcctga catgcagtta
    27001 accgggatcg aaattgcgct agaggccatt gcctgtgcaa agcagtcagc cgctgaactg
    27061 ggcttaacgc gtctgcaatt tcaggcgctg gactccactc agtttgccac cgctcagggg
    27121 gaggtgccgg agctggtgct ggttaacccg ccgcgccgcg gcattggtaa accgctgtgt
    27181 gattatctct caacgatggc accgcgtttt attatctact ccagctgtaa cgcccaaacc
    27241 atggcgaaag atatccgcga actgccaggt taccgcatcg aacgggtaca gctttttgat
    27301 atgttcccgc acaccgcgca ctatgaagtg ctgacgctgc tggtaaaaat gtaaatacat
    27361 cattcaaatc aatatcacct cggcatacat ccatccaaaa taataatcgt gatcgatcga
    27421 ttaataataa ccgatcgatc acggttatat attgctctat ttttgcaaat caaatcacaa
    27481 ataatcaaat aagatcttta attgaactgt tttgattact acagtgcact taccgaatac
    27541 atacagaggc attgcttagc gaaacacaaa atttcctgag acattattaa cgtcagcaga
    27601 aggttcatgc attgacaacg gaaatgtacg cgtatggagg gcgttccgac gatgtttgcc
    27661 aaattcatag atgttattca aacgttttta actgaaccgg caattttaat tggtttgctg
    27721 gtgggtattg gttatgccct tgataagaaa tcgccaataa aaattattac cggcatggtc
    27781 agcgccatgg tgggattaat gatggtgtta tttggtggtt tccagttttc cgcaacattt
    27841 aaaccggtag cagaagccgt aagtaaagcc tatggtgttc acggatattt aatggattcc
    27901 tacgcaatga aagcggcaac gcaaattgca ttgggggata attttggtta tgtcgggtat
    27961 gtatttgtac tggcgttttt taccaacctg ctgctggtat tatttggtcg gtatactggt
    28021 gccaaaggga tatttttgac agggaatacc ggtgtttcac attctcaggc ggtattatgg
    28081 ttgattgtct tttggctggg ttttagctgg actacatcca ttattattgc cggaatatta
    28141 acgggtgtat tctgggcgtt ctccaccacg ctcattgtta agcccattgc caaagttacc
    28201 aaagatgccg gttttaccat tgctcataac cagatgttgg gattgtggtt tttctccaaa
    28261 ttcgcccata aatttggtga ccctgaaaag cacgatgctg aaaacctgaa acttcccggg
    28321 tggctggcga tttttaacca taacgtaacg gctatcgcca ttgtgatgac gctgtttgtc
    28381 ggtggtttct tgctatcaac gggtatcgat aatgtccagt taatggcgaa aggcaagccc
    28441 tggtacatct atatcatcaa ccttggctta cagttctcca tgtatatggt cattctgctg
    28501 cagggtgtgc gcatgatggt gggcgaaatt aacggttcgt ttaaaggctg gcaagatcgc
    28561 tttattccta acgctattcc tgctgtcgat gttgcagcgc tgttaccttt ttcacccaat
    28621 gccgcaacgt taggcttcgt cttctgtact tttggcacca ttttttcgat ggggatctta
    28681 ttactggttc acagcccaat tatggtattg cctggctttg tacctctgtt tttctctggc
    28741 ggtccaattg gcgtattagc aaaccgcatg ggcggatacc gttccgtaat tatctgtacg
    28801 ttcttgctgg gtattattca gaccttcggt acggtgtggg ctattccgtt aaccgggctt
    28861 gctgaaaatg gcgtcggctg gacaggaata tttgactggg caaccgtatg gcctgctatt
    28921 tgtgaagttc tgaaatttat cgctgcaaca ttccatcttg gtccttacgc gggttaatta
    28981 aatattaagg gtttaaaaat gaaagataca attaatattc tgtttgtctg tggttacggt
    29041 gtaggcagta gcgttatgtt acaaacggtc gttaaaaaag cgctgaccaa atatgatttc
    29101 aaagttgaaa tggaacatac cgccgcaggt gaagtcggcg gatttaccga ctgggcggat
    29161 atttttgcta tttccaaaaa attactcgat gtggttagtc ttgacccgaa acaagggcaa
    29221 tatttgattc cgatagagaa tattatggac ggtgaaacta ttggtcagca aatttatgaa
    29281 gtggttcagc aacattttcc gcatttaatt aatcaataag gtagggcgta atgagagcga
    29341 ttattctgct gttcgatagt ctgaataaac gttatctgcc accttatggc gatgcgttaa
    29401 ccaaagcgcc taatttccaa cgtctggcgg ctcatgccgc cacctttgaa aacagttacg
    29461 tcggcagtat gccgtgtatg cctgcccggc gggagttgca taccgggcga tgtaacttcc
    29521 tgcatcggga gtgggggccg ttagaaccgt ttgacgattc catgccggag ctgcttaaaa
    29581 aggcggggat ctatacccac ctgataagcg atcatttgca ttactgggaa gatggcggcg
    29641 ggaattatca taatcgctac agttcgtggg agattgtgcg cggacaggaa ggcgatcact
    29701 ggcatgcaag cgttgcgcaa ccgcctattc ccgaggtgct acgggtgccg caaaagcaga
    29761 ccggtggcgg tgtttctggt ctgtggcgcc atgactgggc aaaccgggaa tatattcagc
    29821 aggaagcgga ttttccacaa actaaagtgt ttgatgcagg ctgtgccttt atccacaaaa
    29881 atcacgcgga agataactgg ttattgcaga tcgaaacgtt tgatccgcac gagccgtttc
    29941 acacaacgga agagtatctt tccttgtatg aagataactg ggatggaccg cattatgact
    30001 ggccgcgtgg ccgggtgcag gaaagcgacg aggccgtgga gcacatccgt tgccggtatc
    30061 gttcgctggt gtcgatgtgc gatcgcaacc tggggcgcat tctcgacctg atggatgagc
    30121 acgatctgtg gcgagatacc atgctgattg tcggcactga ccacggcttt ttactcggtg
    30181 agcatggctg gtgggcgaaa aaccagatgc cttattacaa cgaagttgcc aataatccgt
    30241 tgtttatctg gaacccgcgc agtggtgtaa aaggagagcg gcgacaggca ctggtacaaa
    30301 tgatcgactg ggcgccaacg ttgtatgact tttttcaaca gccagtgccg cccgatgtgc
    30361 aggggcaacc gctggcgaaa acggtcagtc acgatgaacc agtacgcagc tcggcgatgt
    30421 ttggtgtttt cagtggtcat gctaacgtaa ctgatgggcg ttatgtgtat atgcgtgcag
    30481 cgctgccggg gcgtgaggat gatattgcca actacacgtt gatgtcctgc aaaatgaaca
    30541 gccgctatcc ggtggatgag atgcgggctt tatcgctggc cccaccgttt cgttttacca
    30601 aagggttaca ggtattacgc atcccggcac aggaaaaata taaggggttg aatcagtttg
    30661 gtcatttgct gtttgatctg caaaacgatc cgcagcagct acatccgatt catgatgatg
    30721 tgatcgagtc ccggatgatc gcgttgctga ttcagttgat gaaagataat gatgcgccgc
    30781 cagagcagtt tcagcgcctg ggattagcgt agagaagtga ttttgccgga gtgggtgttc
    30841 gcccactccg gcaaatgaat aattattgcg ggaaccactg atcgctgatt tgttgatatg
    30901 taccgtcagc tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat
    30961 ccggacgtac agcgataccc agaccggtgc caaagtattg cggatcggtc actttttcag
    31021 tagcaacgcc cagttgcgga ttggttttca gccattcatt aaccaccgca gtgtcaccaa
    31081 atacgccatc aatacggcta tttttcagat cgataaaggc attttgataa ctgtcatagg
    31141 agaccgtttt cacttccggg tgctgatcct gaatatattt ctggtgcgta gtaccgtttt
    31201 ccatcccaat gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa
    31261 tcacgacggc tgagttttcg taatagggcg tggtgaacga tacctgcttg ctacgatctg
    31321 gtgtgatatc cataccggag attacggcgt catatttcct gaatttcagt gacgggatca
    31381 ggctgtcgaa cgcgtgattg gtaaaagtac attctgcctg catctgtttg cataacgctt
    31441 ttgctaggtc gatatcaaag ccgacgatct ggttattggc atccatcgac tcgaacggcg
    31501 gataggtcgc tgatacgcca aaattgattt tctctgcggc agaagcgccg aacgcacagg
    31561 atgccagtaa tgcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta
    31621 tgattattgg ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa
    31681 taaatatgat taaataattt gcatgaaata aaaaaagacg gacaacttag tgagttgtcc
    31741 gtctttatta taagaattta tgcacta
//
LOCUS       DBFIFS010000035        31382 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.035, whole genome shotgun sequence.
ACCESSION   DBFIFS010000035 DBFIFS010000000
VERSION     DBFIFS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.035"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(80..898)
                     /gene="cof"
                     /locus_tag="N7A07_001467"
     CDS             complement(80..898)
                     /gene="cof"
                     /locus_tag="N7A07_001467"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU2476156.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            998..2698
                     /gene="ybaE"
                     /locus_tag="N7A07_001468"
     CDS             998..2698
                     /gene="ybaE"
                     /locus_tag="N7A07_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU2476157.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2763..3458
                     /gene="queC"
                     /locus_tag="N7A07_001469"
     CDS             2763..3458
                     /gene="queC"
                     /locus_tag="N7A07_001469"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU2476158.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(3510..3908)
                     /gene="fadM"
                     /locus_tag="N7A07_001470"
     CDS             complement(3510..3908)
                     /gene="fadM"
                     /locus_tag="N7A07_001470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU2476159.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4014..4385)
                     /gene="ybaV"
                     /locus_tag="N7A07_001471"
     CDS             complement(4014..4385)
                     /gene="ybaV"
                     /locus_tag="N7A07_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU2476160.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4536..6407)
                     /gene="ppiD"
                     /locus_tag="N7A07_001472"
     CDS             complement(4536..6407)
                     /gene="ppiD"
                     /locus_tag="N7A07_001472"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU2476161.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6599..6889)
                     /gene="hupB"
                     /locus_tag="N7A07_001473"
     CDS             complement(6599..6889)
                     /gene="hupB"
                     /locus_tag="N7A07_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU2476162.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7080..9434)
                     /gene="lon"
                     /locus_tag="N7A07_001474"
     CDS             complement(7080..9434)
                     /gene="lon"
                     /locus_tag="N7A07_001474"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU2476163.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9622..10896)
                     /gene="clpX"
                     /locus_tag="N7A07_001475"
     CDS             complement(9622..10896)
                     /gene="clpX"
                     /locus_tag="N7A07_001475"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU2476164.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11022..11645)
                     /gene="clpP"
                     /locus_tag="N7A07_001476"
     CDS             complement(11022..11645)
                     /gene="clpP"
                     /locus_tag="N7A07_001476"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU2476165.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11890..13188)
                     /gene="tig"
                     /locus_tag="N7A07_001477"
     CDS             complement(11890..13188)
                     /gene="tig"
                     /locus_tag="N7A07_001477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU2476166.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13532..13849)
                     /gene="bolA"
                     /locus_tag="N7A07_001478"
     CDS             complement(13532..13849)
                     /gene="bolA"
                     /locus_tag="N7A07_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU2476167.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14154..14732
                     /gene="yajG"
                     /locus_tag="N7A07_001479"
     CDS             14154..14732
                     /gene="yajG"
                     /locus_tag="N7A07_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2476168.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14776..16251
                     /gene="ampG"
                     /locus_tag="N7A07_001480"
     CDS             14776..16251
                     /gene="ampG"
                     /locus_tag="N7A07_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU2476169.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            16711..17658
                     /gene="cyoA"
                     /locus_tag="N7A07_001481"
     CDS             16711..17658
                     /gene="cyoA"
                     /locus_tag="N7A07_001481"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU2476170.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            17680..19671
                     /gene="cyoB"
                     /locus_tag="N7A07_001482"
     CDS             17680..19671
                     /gene="cyoB"
                     /locus_tag="N7A07_001482"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU2476171.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            19661..20275
                     /gene="cyoC"
                     /locus_tag="N7A07_001483"
     CDS             19661..20275
                     /gene="cyoC"
                     /locus_tag="N7A07_001483"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU2476172.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            20275..20604
                     /gene="cyoD"
                     /locus_tag="N7A07_001484"
     CDS             20275..20604
                     /gene="cyoD"
                     /locus_tag="N7A07_001484"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU2476173.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            20616..21506
                     /gene="cyoE"
                     /locus_tag="N7A07_001485"
     CDS             20616..21506
                     /gene="cyoE"
                     /locus_tag="N7A07_001485"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU2476174.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            21655..23019
                     /gene="yajR"
                     /locus_tag="N7A07_001486"
     CDS             21655..23019
                     /gene="yajR"
                     /locus_tag="N7A07_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476175.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(23147..23638)
                     /gene="yajQ"
                     /locus_tag="N7A07_001487"
     CDS             complement(23147..23638)
                     /gene="yajQ"
                     /locus_tag="N7A07_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU2476176.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            23806..24717
                     /gene="panE"
                     /locus_tag="N7A07_001488"
     CDS             23806..24717
                     /gene="panE"
                     /locus_tag="N7A07_001488"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU2476177.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            24680..25270
                     /gene="yajL"
                     /locus_tag="N7A07_001489"
     CDS             24680..25270
                     /gene="yajL"
                     /locus_tag="N7A07_001489"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU2476178.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(25324..26772)
                     /gene="thiI"
                     /locus_tag="N7A07_001490"
     CDS             complement(25324..26772)
                     /gene="thiI"
                     /locus_tag="N7A07_001490"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU2476179.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            26978..27220
                     /gene="xseB"
                     /locus_tag="N7A07_001491"
     CDS             26978..27220
                     /gene="xseB"
                     /locus_tag="N7A07_001491"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU2476180.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            27220..28119
                     /gene="ispA"
                     /locus_tag="N7A07_001492"
     CDS             27220..28119
                     /gene="ispA"
                     /locus_tag="N7A07_001492"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU2476181.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            28144..30006
                     /gene="dxs"
                     /locus_tag="N7A07_001493"
     CDS             28144..30006
                     /gene="dxs"
                     /locus_tag="N7A07_001493"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU2476182.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            30061..31035
                     /gene="yajO"
                     /locus_tag="N7A07_001494"
     CDS             30061..31035
                     /gene="yajO"
                     /locus_tag="N7A07_001494"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU2476183.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            31266..>31382
                     /locus_tag="N7A07_001495"
     CDS             31266..>31382
                     /locus_tag="N7A07_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476184.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNK"
BASE COUNT         7236 a         8251 c         7858 g         8037 t
ORIGIN      
        1 cggtttaaac accacggcac aaaatgacaa attcaacgca aaaaaaccgg gcaattgccc
       61 ggtgctggaa gggatctcgt tattcggggg aataaggtag atgtggatag tccagccagt
      121 gcgtcaaata gtgagagaca gcctgatttc ggcaatgtcc aatcaccggt aaatgcggga
      181 gctccgcgcg cagttgcggc atcgcattgc ccataataaa tccgctaccg acgctgccta
      241 acatttcgcg atcgttcatc gcatcaccaa aggccatgca atcgcgcaac gataaaccta
      301 aatgttgggt cagcaccgtc agtgcagcgc ctttattgca gcccaccggc agcacttcaa
      361 ggcaatccgt ggcggaaaaa cacaaatgtg cacgctcgcc taatgcttcg tgtagctgga
      421 tctgcaaacg tgtaagatcg tcgtgatcgc cacagaagca gatcttggtg acgctgccga
      481 gtggcatttt tttgacatcg attatttgat aacgaaaacc gctatagaca aatgcctgca
      541 acaacgcagg gatctctttc ccggtaaacc aaccgtcgtc attgaaaata tgcatgctgg
      601 ctcgggtatc ccattgctga tacagcacca gctccgcgac atccgcaggt aaatcatcac
      661 gatgtaaaag ttcaccttcc agagaatgca cgcgcgttcc gttgccggta atcaaatacg
      721 catccagcga tatcgccccg agaatatgct gcatctccag cgcatgacgc cccgtggcaa
      781 aagtgagggt aatgtcgcgt tcacgcagtc gcgccaaagt agagagggtt ttctcaccta
      841 aatgatggtc aggcatcaat aaagtgccat ccatatcaaa tgctgccaga cgagccattt
      901 ctttctccac tctgtgacac ggttaatggt gattgagtat cacctgatat atacggaagt
      961 aatagtgaat agttaaataa tattgttccg ggtttttatg cgactgctca accgccttaa
     1021 ccagtatcaa cgtctgtggc aaccttccgc cggaaagccg caaaccgtca ccgtcagcga
     1081 actggccgaa cgctgttttt gcagcgaacg ccatgttcgt acgctgttgc gtcaggcaca
     1141 ggaagcggga tggctggagt ggcaggcgca gtcaggacgc ggaaagcgcg gacaattacg
     1201 ctttctggtc acgccagaat cgctacgcaa tgcgatgatg gaaaaggcac tggaaaccgg
     1261 aaagcagcaa gatgtgctgg agctggcgca actggcccca ggtgagctgc gcactctgtt
     1321 acagccgttt atgggcggac aatggcaaaa cgatacaccc acgttgcgta ttccctacta
     1381 tcgcccgctc gaaccgctac aaccgggctt tttgcccggc cgtgccgagc agcatctcgc
     1441 cggacagata ttttccggcc tgacccgctt cgataataat acccagcgcc cgattggcga
     1501 tttagcgcat cactgggaaa cctctgctga cgggttacgc tgggactttt atcttcgttc
     1561 aaccctacac tggcataacg gcgatgcagt aaaagcctca cacttacacc agcgattatt
     1621 gatgctgtta caactgccag cactggatca attatttatt agcgtgaagc gtattgaggt
     1681 cacccatccg cagtgtctga ccttcttttt acatcgcccc gattactggc ttgcgcaccg
     1741 gctggcgagc tattgcagcc atctggcgca tccgcaattc ccactgattg gcacgggtcc
     1801 ttttcgctta acacaattca cagctgaact ggtgcgcctg gaaagccatg attattacca
     1861 tttacgtcat ccgctgctta aagcggttga gtactggata actccgccgc ttttcgaaaa
     1921 agatttggga accagttgtc ggcatcccgt gcaaatcacc atcggcaaac cggaggagct
     1981 gcaacgggtc agccaggtca gtagtggcat cagtttaggt ttttgctatt taacgttgcg
     2041 caaaagtccc cgactctccc tctggcaggc gcgaaaagtg atctccatta ttcatcaatc
     2101 cggtttatta caaacgttag aagtcggaga aaacctgatc accgccagtc atgcattact
     2161 gccaggctgg actattccgc attggcaagt accggatgaa gtcaaactac cgaaaacctt
     2221 gacgctggtt tatcacctcc cgatagaact tcataccatg gcagaacgcc tacaggcgac
     2281 actggcagcg gaaggctgtg aactcacaat tatttttcat aacgcaaaaa actgggacga
     2341 cacgacccta ctggcacacg cagacctcat gatgggcgac agattaattg gcgaagcacc
     2401 ggaatatact ctggagcaat ggctgcgctg cgatccgctg tggccacatg ttttcgacgc
     2461 tccagcatac gcacatctac aatcgacact ggacgcggtg caagtaatgc ctgatgagga
     2521 aaaccgattt aatgccctga aagcggtttt tagccagtta atgacagatg cgacgctgac
     2581 gccgctgttc aactatcact atcgcattag tgcccctccc ggcgtgaacg gtgtgcgact
     2641 gacaccgcgc ggctggtttg aatttaccga agcctggctt cccgcgccgt cgcaatgaat
     2701 agctggtccg ggttaatcgc aggcgttacc ataacgcttt tattgttcaa gcaggattat
     2761 ctatgaaacg tgctgtcgtt gtgttcagtg gaggccagga ttccaccacc tgtctggtgc
     2821 aggcattaca acaatatgat gaagtccatt gcgtgacgtt cgattacggt cagcgccatc
     2881 gcgcagaaat cgacgtggca cgcgaactgg cgctgaaact gggggcacgc gcgcataagg
     2941 ttcttgatgt aacgctgctc aacgagctgg cggtcagcag cctgacgcgt gacagcattc
     3001 cggtgcctga ttatgaacct gaagccgatg gcatcccgaa tacgtttgtc ccagggcgta
     3061 atattttgtt cctgacgctg gcggcaatat atgcgtatca ggtaaaagca gaagccgtaa
     3121 ttaccggcgt ctgcgaaacg gatttctccg gctacccgga ttgccgcgat gagtttgtga
     3181 aagtactaaa ccatgccgtc agtttgggca tggcgaaaga tattcgtttt gaaacgccgc
     3241 tgatgtggat tgataaagcg gaaacctggg cgctggcaga ttattacggc aaactggatt
     3301 tagtccgtaa cgaaacgttg acctgctata acggcattaa aggcgacggt tgcggtcatt
     3361 gtgcggcatg taatttacgc gccaacggtt tgaatcatta tctggccgat aaaccgacgg
     3421 tgatggcagc gatgaagcag aaaaccgggt tgaagtaatt attccgggtg tcgccggatg
     3481 cggcttgagc atccggcacc acaaaacgtt tacttaacca tctgctccag cttttcgcgc
     3541 aattcccctt ccagagctaa tgctttctgc gttttaagat caatacaaac aaacgtaata
     3601 agcgcatccg ctaccacctg cccttccggc tccagtgtaa tgacctggct taagatgccg
     3661 cttttaccgt ttaattgctg taactggctc gtaatggtta acaagtcgct taataccgcc
     3721 ggacgacgat agttaatatt gatattcacc accacgaaag cgatgttatg ggccgtcatc
     3781 cagtgaaaac tgtcgctatt ttccaaccca tcccagcggg cttcttcgag aaattcaagg
     3841 tagcgggcgt tgtttacgtg ctggtaaacg tcgagatgat atccacgaac tttgatttgt
     3901 gtttgcatag cgcaataacc ttacgttgtt tttataaaaa ctgttataag tacagaggtc
     3961 ataactggta tgacctcttc agtctggcaa aaaattgcca ctatgcaaat taattacagg
     4021 gttaataccg ccagattacg ttccaccagc gaattgccca tccccggcac ctgctttaga
     4081 tcctccaccg ttttaaacgg accgtactct tcgcgataac tgacaatcgc ttgcgctttc
     4141 ttcaggccaa cgccattcat cgcgcgggct agctcttccg cgctggcatt attaatgctg
     4201 acccgggagc cttcttcgtc actggctttc gccggtactg ctgctttatt ttgtgctgca
     4261 ggagcttccg ctttggtttc taccgccgtc ggtttcgcca cagaagctgc cgccagcgcg
     4321 ctatgagaca ttccggcaca ggccagggac agggtaatga gcagtgcttt aattccgtgt
     4381 ttcatactgt tttctccttg tttgttaaca gtggaatcac cttaacggca gtgaaaacag
     4441 taaacaaatg gcaatgttca gaaatggaaa aggccgcgaa agcgaccttt tcttgttaca
     4501 gctgcaaagc atttttttgc gaggcggctt caggattatt gctgttccaa cgcatcgcca
     4561 attttgattt tcgcctcttt acgcaggtta ctcatcagag cttcaaagac gatttgtgcg
     4621 ttgttctggg tgataccctg caccatcgct tttttctgat cttccggcat tgaaccttgt
     4681 ttcacttcat ccagcgccag cagaaccaca ttaccttgca tatcggtcgc cataccgtag
     4741 ctcggtttgt ctttcgctgg cagtggcagt gcaaacgccg cctggctaat cgggtcacga
     4801 ccggaacggc ttaaggtttt cggctcgcca aatttcagac cggcagcctg catagcttcc
     4861 gcacctttgc cggctttcaa atcaaccagc agtttctcag catccacttt cgcctgttgt
     4921 tcagctttgt tgtgctgaac cagtgcctta acttgttcct gaacatctgc caacggtttc
     4981 accgcttccg gtttgtgctc gctgatgcgc agcacgaatg cgcggtcgcc gtctacggtg
     5041 atgatgtcag agttgatgcc cggcgcgccg ttttcaccta ccagaccgcc gttaaagata
     5101 gcgtcagcaa ccggcttgaa gttcaactct tccggcaggt tctctttgct gaaccaaccc
     5161 gtctgagtgg ctttaacgcc ggcagcttgc tctgcaccgg ccagagactc ggtgtcgttg
     5221 cttgccgcat cgctcacttt ctgctgcagc gcgtagtacg catcgagggc tttttcgtgt
     5281 ttcactttcg ccgcgacatc gtcacgtact tcgtctaacg atttcacttt cgctggctga
     5341 atgtcgtcca gacgtacaat caggaaaccg accgaagatt tgatgacacc agacagttgg
     5401 cctttttctt tcagaccagc atttttcagt tcatccggga tagtggcatc ttctaaccaa
     5461 cccatatcgc cgccgttacg agcagagata atatcggcag atttttcttt ggctaatgca
     5521 gcaaaatcac cgcctttatt cagctcatca agtaccgctt tcgcttcatc ttcagttttg
     5581 gtctggatga tgctgtaacg ggtacgctgc ggctgggtga attgatcctg atgctgatcg
     5641 tagtagctct ggagatccgc atcgctaacc ggttgctgca tcgttgcggc atccagcttg
     5701 atgtaactca cgcggaattg ttccggcgtc atgaaattgt ttttgttttg ttcgtagtag
     5761 ctggcaattt cctgttcggt cacaggctgc ttcgccgcca gcgcgttaac atcgatagtc
     5821 gcttcacgca ccacgcgttg ttgagcgacc agtgccgcca gctcgtcggt ttcacctttc
     5881 agcataaaat cggtaccagc aacgccgtta atcagctgtt gggtagtgag ctggttacgc
     5941 agcgcctggg cgtactgatc ggcggtcatc cccatctggt tgaggatacc gttatagcgg
     6001 ctgttatcaa atttgccatc aacctggaag gctggggtcg cgaaaatcgc ctgtttaacc
     6061 tgctcatcgc tgatacccag tttcagctca cgtgcgtact gatccagcag cgcctcgtcg
     6121 atcagacgat tcagcacctg ttgacgcagg gttttcatat agccttcgtt cgctgccagc
     6181 tcggagtatt gatcgcccag ctgttgctgc atgcgattac gctcgctgtt gaaggcattc
     6241 tcgaattgcc cacggctgat ttcctggtca ttcacttttg cggcgtagtt attgcctccg
     6301 ccaatcaggt aaccactcac gccggtcaat atgaacgaca cgataatgat accgaaaata
     6361 atcttgagca cgagactgtt tgcagccgtg cgtaagctgt ccatcatggt gtaacaacac
     6421 tccgctgtag atgactgtgt acctcacgca acgtatcgat gcgcgcaaag gcctattgtg
     6481 acaagaaacg ggggcaattg tcagcccaca agacgcagaa agtcaccgaa tacaaataaa
     6541 aaaggcacat cagtagatgc gcccttgaac ttcgtcacat ccccactggg gacaacgctt
     6601 agtttaccgc gtctttcagt gctttacctg cacggaagct cggtacttta gcggcagcga
     6661 tggtgatctc tttaccggtc tgcgggttgc ggccagtacg ggcagcacgc tctttaacgg
     6721 caaaagtacc aaaacctacc agtgctacat catccccttc tttcagagat tcagttacgg
     6781 aagcaataat agcatctaac gcacggccag ccgcagcttt agagatatca gcccctgcag
     6841 caatcttgtc gatcaattga gatttattca ctcttctctt cctctttata atttatatcg
     6901 cacttgaatc cttcaaggta cgaacgcgca gcagttatat caggccagcc atccccttac
     6961 aacacccctt aataagggca agcccgatcc gccatctaac ttagcgagac aaaaaaaggc
     7021 tggcaagccc gaattagcct gccagccctg tttttattag tgcattttgc gcgaggtcac
     7081 tattttgcag tcacaacctg catgccagac ggttcatttt gcagcgccag agtcagaact
     7141 tcctcaatgc gcttcacagg atgaatgtcc agatcggcaa ttacgttgtc aggaatctct
     7201 tccagatcgc gtttattttc gaacggaatt aacactgttt taatcccgcc gcgatgcgct
     7261 gccagtagtt tttctttcaa accaccgatc ggcagtacct gaccacgcag agtgatctca
     7321 ccggtcattg ccacatcggc acgaaccggg ttaccggtca ggcaagaaac cagcgcggtg
     7381 cacatagcaa taccggcact cggaccatct ttcggcgtcg caccttccgg tacgtggacg
     7441 tggatgtcac gtttttcgta aaaatcaggg ttgatcccca gtttttccgc acgcgcgcga
     7501 accaccgtta acgcagcctg aatagactcc tgcatcactt cgccgagcga accggtatag
     7561 gtcagtttgc ctttacccgg aacacatgcg gtttcaatgg tcagcaagtc accgcccact
     7621 tccgtccacg ccagaccggt tacctgaccg acacggtttt cgttatcagc gcgaccatag
     7681 tcgaaacgct gaacaccaag gtagtcatgc aggttgtcgc cgttaatttc gatatgtttt
     7741 aatgacttat cgagcagtaa ctgcttaacc gctttacggc acagcttgga gatttcacgc
     7801 tccagaccac gcacgcccgc ctcacgggtg tagtaacgaa taatgccgat aatggcgcta
     7861 tcgtcgacgg tcagctcacc ttttttcagt gcattacgtt caatctgctt cggcagcagg
     7921 tgacgtttgg cgatgttcag tttttcatct tcggtataac cggagaggcg aatcacttcc
     7981 atacgatcca gcagtggtgc cggaatgttc atggagttcg acgtcgcgac aaacatcacg
     8041 tcgctgagat cgtaatccac ttccaggtag tgatcgctga aggccacgtt ctgctctgga
     8101 tccagcactt caagcagtgc ggaagccgga tcgccacgca tgtcagaaga cattttgtcg
     8161 atctcatcga gcaggaacag cgggttttta acgcccactt tcgccatttt ctggatcaat
     8221 ttacccggca tagaaccgat gtaagtacgg cggtgaccac ggatttccgc ttcatcacgc
     8281 acgccgccca gcgccatacg gacatattta cgcccggtgg ctttggcaat ggactgaccc
     8341 agggaggttt tacctacccc cggcggccct accaggcaaa ggatcggtcc cttgattttg
     8401 ttgacacggc tttgaaccgc aagatactca aggatgcgat ctttcacgcg ctccagacca
     8461 taatggtcgg tattaaggat ttcctgcgcc tgacgcaggt cttttttgac cttgctgcgc
     8521 gcattccacg gtacctgtac catccagtcg atataaccac gcactacggt cgcttctgcc
     8581 gacatcggag acatcatttt cagcttctgc aactctgctt ccgctttctc ttttgcctct
     8641 ttcggcatct tcgccgcgtc gattttgcgc ttcagggctt cgttttcgtc cggcgcgtcg
     8701 tccatctcac cgagttcttt ctgaatagct ttcatttgct cgttcagata gtactcacgc
     8761 tgggatttct ccatctgctt tttaacgcgg ttgcgaatgc gtttctcaac ctgcagcaga
     8821 tcgatttccg attccatcat tgccatcaga tattccagac gttcgttaac gtcggacatc
     8881 tccagaacgg actgtttgtc agccagtttc agcggcatat gtgcagcaat ggtatccgcc
     8941 agacgcgccg gatcgtcgat gctattcaac gacgtcagca cttctggtgg gatttttttg
     9001 ttcagcttga tgtagccttc gaactggctg attgcagtac gcaccagcac ttcctgttct
     9061 cgctcatcaa tggtcggcga ctccagatac tccgccttcg cagaaaagtg ttcgccattg
     9121 tcagagagcg cagaaatacg cgcgcgctgt aacccctcga ccagcacttt gacggtgccg
     9181 tcaggcagtt tcagcatctg caatatagag gccacggtcc cgacggtgaa aagatcgttt
     9241 acacccggct catccgttga agcttctttc tgcgcgacca gcataatttt tttatcatgg
     9301 tccatcgccg cttccagaca acggatagat ttttcccgcc cgacaaataa ggggatgacc
     9361 atgtgcggat aaaccaccac atcgcgcagc ggcaatacgg ggatttcaat gcgttcagaa
     9421 cgctcaggat tcatagagct ctctcttagt ttaatttccg ccaggtaatc agatgacacg
     9481 actgtgcttc acgccatcta ttaacatgta cgtcagtata tggggatgtt tcccccacat
     9541 tcaacgccga gaatagagga aaaattaaag gggagataaa atcccccctt tttggttaac
     9601 taattgtatg ggaatggtta attattcacc agatgcctgt tgcgcttccg gcttgccata
     9661 aatcagcaac ggtttgcttt gaccatcaat taccgactcg tcgataacca ctttttcgac
     9721 atcttccatg gacggcagat cgtacatggt atcgagcagt gcggcttcta cgatggaacg
     9781 caggccacgg gcaccggttt tacgcgccat cgctttctta gcgatagcat ccagcgcctc
     9841 gtcacggaat tccagatcca cgccttccag attaaacagc gcctgatact gcttggtcag
     9901 ggcgtttttc ggctctttga ggatctgaat cagagcttct tcgctcagtt cattcaacgt
     9961 tgcgacaacc ggcagacgac caataaactc agggataaga ccaaacttga tcagatcttc
    10021 cggttcaacc tgcgccagca gctcgccttc gcttgctttg tcggacttcg cttttaccgt
    10081 cgcgccaaaa ccaatgccgg agccggtttc tacacgatgg gaaatcactt tatccagacc
    10141 ggcaaacgca ccgccacaaa taaacaggat cttagaggta tcaacctgca agaattcctg
    10201 ctgcggatgt ttacgtccac cttgcggtgg aacagcagct accgtacctt cgatcagttt
    10261 caacagtgcc tgctgtacgc cttcaccgga aacgtcacgg gtaatagacg ggttgtctga
    10321 cttacgagaa atcttgtcga tttcatcgat gtagacaatc ccgcgctgcg ctttctgtac
    10381 gtcgtaatcg cacttttgca acagcttctg aatgatgttt tcaacgtctt cgcccacata
    10441 accggcttcg gtcagcgtgg ttgcgtcggc catggtgaac gggacgtcca gcaggcgcgc
    10501 cagcgtttca gccagcagcg ttttaccgga accggtcgga ccgatcagca gaatgttact
    10561 tttgcccaac tcgacgccat tgctggtatc gccgttgcgc agacgtttgt agtggttgta
    10621 taccgcgacc gccagcactt ttttcgcctg ttcctggccg ataacgtaat cgtccaggtg
    10681 gttacgaatt tcatgcggcg tcggtagcgc actgcgttca cgatgcggtg caacttcttt
    10741 aatctcttcg cgaatgatgt cgttacataa atcaacacat tcgtcgcaga tatacacgga
    10801 tggaccggca atcagcttgc gcacttcatg ctggcttttg ccgcaaaaag agcaatacag
    10861 caatttgcct gagccatctt tgcgtttatc tgtcatgggt caaaacctct tctttgttct
    10921 ttgtgccgca cacgacgacg caaatgccat tctcaggcgc aagccgctta cagcgttgtg
    10981 ccgccctgga taagtatagc ggcacagttg cgcctctggc atcaattacg atgggtcaga
    11041 atcgaatcga ccagaccgta ttccaccgct tcaggggcgg aaaggaagcg atcgcgctcg
    11101 gtatcacgtt caatctgttc taatgattga cccgtatgaa gcgccataag ttcattcata
    11161 cgccctttaa ctttcagaat ttcacgggca tgaatttcga tatcggtcgc ctggccctgg
    11221 tagccgccca acggttggtg aatcatcacg cgcgaattcg gcaggcaaaa acgtttacct
    11281 tttgcccctg cggtcagcaa gaaagcgccc atcgaggccg cctggcccat acagatggtg
    11341 ctgacatcag gcttgataaa ctgcatggtg tcatagatag acatcccggc agtgatcacc
    11401 ccgcctgggg agttaatgta cagatagata tctttttctg ggttttctgc ttccaggaac
    11461 agcatctgag ccacaatcag gttagccata tggtcttcaa cctggccagt cagaaaaatg
    11521 acgcgttcct taagtagacg agaatagata tcaaaagagc gctcaccacg tgaggtctgt
    11581 tcaatgacca tcggcaccag cgccatatgg ggtgcaaagt tatctcgttc gccgctgtat
    11641 gacatttccg tctcctggat aaaattgaaa aaacctgctg taccgattgt aaccttatgg
    11701 acggattatc agctagtccc tgtgttatgg gtacggcatc accatatttc aagcataaca
    11761 atcttttgtt gttacgctaa caccgaaaga ggcttttcgc acggttccat gcaaaattca
    11821 tgacaaaaaa agcccaccac ctggcggtga cgggcctttg tgcgaattta gcgcgttatg
    11881 ctgcgtaaat tacgcctgct ggttcatcag ctcgttgaaa gtggtttctt tttcagtcac
    11941 tttcgctttc gccagtacag cttcaacagc ctgttcttcc agagcaacat tgcgcatgtt
    12001 gtccatcagt tctttgtttt tgctgtagaa ctcgataact tctttcggat cttcgtacgc
    12061 tgaagccatc tcttcgatca ggcctttcac gcgctcttcg tcagctttca gctcgttggt
    12121 gcggataact tcgcccagca gcaggccaac aactacgcgg cgtttagcct gttcttcgaa
    12181 cagttcgcgc ggcagttcca gagcttgttt ttcgttgcca ccgaaacgct gtgcagcctg
    12241 gcgacgcaga acgtcgattt cgctgtcaat aagcgcagcc ggtacatcga tgtcgttagc
    12301 ttttaccaga ccttcgatcg cctgagactt aacgcggtta cggatggcgc tcttcagctc
    12361 gcgctccatg tttttacgca cttcagcgcg cagaccttct acggaaccat cttcaacgcc
    12421 gaaacgtttg atgaattctg cagtcagttc cggcagttca cgctcttcaa ctttcttcag
    12481 gttgatagcg aatttcgctg ctttaccttt caggttttct gcgtggtatt cttccgggaa
    12541 ggtcacgtcg atggtgaact cttcgccagc tttgtggcct ttgataccgt cttcaaagcc
    12601 cgggatcata cgaccctggc ccatcgccag tacgaaatca gacgctttac cgccttcgaa
    12661 ctcttcgccg tctacagaac cggtgaagtc gatggttacg cggtcttctg cttcaacagc
    12721 gccgtctttt tctttccagg tcgcctgctg tttacgcaga gtatccagca tgccgtcaac
    12781 gtcagcgtcg gtcacttcaa cgatcggttt ttcaacttcg atcgcttcca gaccctgcag
    12841 ttcaacttcc ggataaactt caaactctac agagtaggtg aagtcttcac ccagcttgta
    12901 ttcgcccgga acataagtcg gtgcgccagc cggattgatt ttttctttaa tgatggcgtc
    12961 aatgaagtta cggctcatca ggtcacccag aacgtcctgg cgtacagacg cgccataacg
    13021 ctgagcaacg atattcattg gcactttgcc tttgcggaag ccgtcaatac gtactttttt
    13081 cgcaacgttg accagctcgc ttttaacagc ggtctcgatg ctgtcagcag cgatagtaat
    13141 cgttacacgg cggccaaggc cttgagtggt ttcaactgaa acttgcatct tgttacctca
    13201 aaaaatcaca gtgctcggtc aactcttacc ccgcaagcac aggttgttgg cttcgcggaa
    13261 ccgggatgtt cttataatca atcacattcc ctgttaccag aatcatcccg aaagcattca
    13321 aataggacgc ggcattatag cggcatcact tttatgagtc gagaacggtt attgcgcgct
    13381 gtcgcgtatt ttttcacaat tcccgggcaa ttttcgtctg aatgctgggc aaaaagcgca
    13441 aaatccagac aaaacaaaac ggcccgcagg ccgtttttca taaaatcttt agcaacatac
    13501 tggaaaagcg ccgacagttg caaatgcgtt tttacgcgat gcttcctgct ccacgacagg
    13561 gaggagaggc aaagacggtg tcctgcaacc cttcccactc cttaatagtg taagtatgca
    13621 gagccagcgc atgaacggta gtagagagtt cctccgctaa agtactgtaa atcattcgat
    13681 gacgattcag aaaacgttca cccgtaaaac gatcgctgac cagcacaact ttaaaatggc
    13741 tttcagagcc ggctgggaca ttgtgacgat agctttcatc cactacttcg aggaatacgg
    13801 gttggaacgc cgcccttaat ttttcttcta tccgctcacg tatcatcatg aaatatctcc
    13861 tccgacaacg ctgagatgtc atccatccct ttaaatacta gccgctttta ccgtttccat
    13921 tgcagcttaa caacaaatat ttaggtttcg tcctggtttt tccgtcaagc gtatgaagtt
    13981 tactgattta aagattgaaa cacaggcctc gcatcagcgt ttcttttacc aggggcgatt
    14041 atttcgtcaa catgatgaat ttacatgcct tacctacttc ccctcgccgt tggcaatgtt
    14101 atgatggcgg gaatttttac caaacacgct taagagtctc tgagagcccg aacatgttca
    14161 aaaaaatcct cttcccgtta gttgctctgt ttatgcttgc aggatgcgca agaccgccaa
    14221 caactattga agtttccccg acgattacac tgccacagca ggatccaagc ctgatgggcg
    14281 tcaccgtaag cattaatggt gccgatcagc gtaccgatca ggcgctggca aaagtcaccc
    14341 gcgataatca aatcgttacc ctgaccgcct cccgcgatct gcgtttcctg ctgcaagaag
    14401 tgctggaaaa acagatgacc gcgcgtggtt acatggttgg tccgaatggc ccggttaatc
    14461 tgcaaatcat cgttagccag ctgtatgctg acgtgtccca gggcaacgtg cgctacaaca
    14521 tcgctaccaa agcggatatc gccatcatcg ctaccgcgca gaatggcaac aaaatgacca
    14581 aaaactatcg tgccagctac aacgttgaag gtgcgttcca ggcctccaac aaaaatatcg
    14641 ccgatgcggt taacagtgtg ctgaccgata ccatcgctga tatgtctcag gacaccagca
    14701 ttcacgaatt catcaagcag aacgcgcgtt aatttctgcc ctctggcccg gtgcaagccg
    14761 ggcctgtaga cgcccatgtc cagtcaatat ttacgtattt ttcaacagcc gcgttcagcc
    14821 atattgctga tcctgggttt tgcttccggg ctaccgctcg ccctgacatc cggcacctta
    14881 caggcctgga tgacggtcga gaatatcgat ctcaaaacca ttggtttctt ctctctggta
    14941 ggccaggctt acgtttttaa attcctctgg tcaccgctga tggaccgcta cacgccccca
    15001 ttttttgggc ggcggcgcgg ttggctgctc gccacgcaaa tcctgttatt agtcgccatt
    15061 gcggcgatgg gttttctcga accaggcacc caactccgct ggatggcggc gctggcggtg
    15121 gtgatcgctt tttgctctgc ctcccaggat attgtcttcg atgcgtggaa aaccgatgtg
    15181 ctcccggcag aagaacgtgg tgcgggcgcg gcaatcagcg ttctgggtta ccgtttaggg
    15241 atgctggttt ccggcggcct agccctgtgg ctggcagata aatggctggg ctggcagggc
    15301 atgtactggt tgatggcggc gctgttgatc ccctgtatta tcgcgacgtt gcttgcacca
    15361 gaaccaaccg acaccattcc tgtgcccaaa acgctggaac aagcggttgt tgcacctctg
    15421 cgagatttct ttggtcgcaa taatgcctgg cttattttgc ttcttatcgt gctgtataag
    15481 ctgggcgacg cattcgccat gagcctgaca accacgtttt tgattcgcgg cgtcgggttt
    15541 gatgcgggtg aagtaggcgt ggtaaacaaa acgcttggct tactagcgac cattgttggc
    15601 gcattgtacg gtgggatttt gatgcagcgc ctgtcactgt tccgggcact gctgattttc
    15661 ggcattttac aaggtgcgtc taacgctggt tactggctgc tgtcgattac tgataagcat
    15721 ctctacagca tgggcgcagc cgtctttttc gaaaacctct gtggcgggat gggcacatca
    15781 gcctttgtcg cgctgttaat gacgctatgt aataagtcat tttccgctac tcaatttgcc
    15841 ctgctctcag cgctttctgc tgtagggcga gtttatgtcg gccccgtggc gggttggttt
    15901 gttgaagcac acggctggtc gacattctat ctattctccg tcgccgctgc cgtaccaggg
    15961 cttattttgc tgctggtttg ccgccagacg cttgaatata cacgagtaaa tgacaacttt
    16021 atctcccgta ccgcatatcc ggcaggttat gcctttgcca tgtggacact ggcggcgggc
    16081 gtcagcctgt tggccgtgtg gttactgcta ttgacgatgg acgcgctgga tttgacgcac
    16141 ttctctttcc tgcctgctct gctggaagtc ggggttttag tcgccctttc tggcgtcgtg
    16201 cttggtggtt tgctggatta tctggcgcta cgaaaaacgc atctgacgta atctgtaaat
    16261 attatttagc tgtagtaatc actcgccgta attattacgg ctaaataaac atcagcatcg
    16321 cttattaata tttgttcttt tgtgcggctt agcgtttgga aattattggc gccatttata
    16381 aattctattt ttcttacacg attcagctaa tgagtcttta ttttctcacc accaagttgt
    16441 cactccaatg ataattattt gttaaataat tgttttattt cgcattgatt ataccaattg
    16501 cccgtccaga cttcttctgc actccccttt tgttataacg cccttttgca acagcttctt
    16561 aaaatcaacc tgatatgttt tgcaacatat gtgacctgac agccaaatcc aagtaacagg
    16621 aatttaatca tgtttacagt aatgtaacct tcccgtaaaa tgcccacaca ttttaaacgc
    16681 caccagatcc cgtggaattg aggtcgttaa atgagactca ggaaatacaa taaaagtttg
    16741 ggatggttgt cattatttgc aggcactgta ttgctcagtg gctgtaattc tgcgctgtta
    16801 gatcccaaag gacagattgg tctggagcaa cgttcactga tactgacggc atttggcctg
    16861 atgttgattg tcgttattcc cgcaatcttg atggctgttg gtttcgcctg gaagtaccgt
    16921 gcgagcaata aagatgctaa gtacagcccg aactggtcac actccaataa agtggaagct
    16981 gtggtctgga cggtacctat cttaatcatc atcttccttg cggtactgac ctggaaaacc
    17041 actcacgctc ttgagcctag caagccgctg gcacacgacg agaagcccat taccatcgaa
    17101 gtggtttcca tggactggaa atggttcttc atctacccgg aacagggctt tgctaccgtg
    17161 aatgaaatcg ctttcccggc gaacactccg gtgtacttca aagtgacctc caactccgtg
    17221 atgaactcct tcttcattcc gcgtctgggt agccagattt atgccatggc cggtatgcag
    17281 actcgcctgc atctgatcgc caacgaaccc ggcacttatg acggtatctc cgccagctac
    17341 agcggcccgg gcttctcagg catgaagttc aaagctattg caacaccgga tcgcgccgca
    17401 ttcgaccagt gggtcgcaaa agcgaaacag tcgccgaact ccatgtctga catggcagcg
    17461 ttcgaaaaac tggccgcgcc tagcgaatac aaccaggtgg aatatttctc caacgtgaaa
    17521 ccaaacttgt ttgccgatgt aattaacaag tttatggctc acggtaagag catggacatg
    17581 acccagccag aaggtgagca cagcgcacac gaaggtatgg aaggcatgga catgagccac
    17641 gcggaatccg cccattaaag gggttgagga agaataaaga tgttcggaaa attatcactt
    17701 gatgcagtcc cgttccatga acctatcgtc atggttacga tcgctggcat tattttggga
    17761 ggtctggcgc tcgttggcct gatcacttac ttcggtaagt ggacctacct gtggaaagag
    17821 tggctgacct ccgtcgacca taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg
    17881 atgttgctgc gtggttttgc tgacgccatt atgatgcgta gccagcaggc tcttgcctcg
    17941 gcgggcgaag cgggcttcct gccacctcac cactacgatc agatcttcac cgcgcacggc
    18001 gtgattatga tcttcttcgt ggcgatgcct ttcgttatcg gtctgatgaa cttggtggtt
    18061 ccgctgcaga tcggcgcgcg tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg
    18121 tttaccgttg ttggtgtgat tctggttaac gtttctctcg gcgtgggcga atttgcgcag
    18181 accggctggc tggcctatcc accgctatcg ggaatagagt acagtccggg agtcggtgtc
    18241 gattactgga tatggagtct ccagctatcc ggtataggta cgacgcttac cggtatcaac
    18301 ttcttcgtta ccattctgaa gatgcgcgca ccgggcatga ccatgttcaa gatgccagta
    18361 tttacctggg catcactgtg cgcgaacgta ctgattattg cttccttccc aattctgacg
    18421 gttaccgtcg cgttgttgac cctggatcgc tatctgggca cccatttctt taccaacgat
    18481 atgggtggca acatgatgat gtacatcaac ctgatttggg cctggggcca cccggaagtt
    18541 tacatcctga tcctgcctgt tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt
    18601 aaacgtctgt ttggttatac ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg
    18661 ttcatcgttt ggctgcacca cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc
    18721 tttggtatca ccacaatgat tatcgccatc ccgaccgggg tgaagatctt caactggctg
    18781 ttcaccatgt atcagggccg catcgtgttc cattctgcga tgctgtggac tatcggtttt
    18841 atcgtcacct tctcggtggg cggtatgact ggcgtgctgc tggcagtacc gggcgcggac
    18901 ttcgttctac ataacagcct gttcctgatt gcgcacttcc ataacgtgat catcggcggc
    18961 gtggtcttcg gctgcttcgc agggatgacc tactggtggc ctaaagcgtt cggtttcaaa
    19021 ctgaacgaaa cctggggtaa acgcgcgttt tggttctgga tcatcggctt cttcgttgcc
    19081 tttatgccac tgtacgcgct gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt
    19141 gacccgcagt tccacaccat gctgatgatt gcagccagcg gtgcagtact gattgcgcta
    19201 ggtattctct gcctcgttat tcagatgtac gtttctattc gtgaccgcga ccagaaccgt
    19261 gacctgactg gcgacccgtg gggtggccgt acgctggagt gggcaacctc ttccccgcct
    19321 ccgttctata actttgccgt tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg
    19381 aaagagaaag gcgaagcgta taaaaagcct gaccactatg aagaaattca tatgccgaaa
    19441 aacagcggtg caggtatcgt cattgcggct ttctccacca tcttcggttt cgccatgatc
    19501 tggcatatct ggtggctggc gattgttggc ttcgcaggca tgatcatcac ctggatcgtg
    19561 aaaagcttcg acgaggacgt ggattactac gtgccggtgg cagaaatcga aaaactggaa
    19621 aaccagcatt tcgatgagat tactaaggca gggctgaaaa atggcaactg atactttgac
    19681 gcacgcgact gtccacgcgc acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt
    19741 cggattttgg atctacctga tgagcgactg cattctgttc tctatcttgt ttgctaccta
    19801 tgccgttctg gtgaacggca ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc
    19861 gttcgttctg gttgaaactt tcttgctgtt gttcagctcc atcacctacg gcatggcggc
    19921 tatcgccatg tacaaaaaca acaaaagcca ggttatctcc tggctggcgt tgacctggtt
    19981 gtttggcgcc ggatttatcg ggatggaaat ctacgaattc catcacctga ttgttaacgg
    20041 catgggtccg gatcgcagcg gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg
    20101 tctgcacgtc acttccggtc ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg
    20161 cggcctgacc agcactaacc gtacccgcat catgtgcctg agcctgttct ggcacttcct
    20221 ggatgtggtt tggatctgtg tgttcactgt tgtttatctg atgggggcga tgtaatgagt
    20281 cattctaccg atcacagcgg cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt
    20341 atcctgtcga tcattctgac ggtgattccg ttctggatgg tgatgacagg ggctgcctct
    20401 ccggccgtaa ttctgggaac aatcctggca atggcagtgg tacaggttct ggtgcatctg
    20461 gtgtgcttcc tgcacatgaa taccaaatca gatgaaggct ggaacatgac ggcgtttgtc
    20521 ttcaccgtgc taatcatcgc tatcctagtt gtaggctcca tctggattat gtggaacctc
    20581 aactacaaca tgatgatgca ctaagagcgg cggttatgat gtttaagcaa tacctgcaag
    20641 taacgaaacc aggcatcatc tttggcaacc tgatctcggt gattggggga ttcctgctgg
    20701 cctcaaaggg cagcattgat tatcccctgt ttatctacac gctggttggg gtgtcactgg
    20761 ttgtggcgtc gggttgtgtg tttaacaact acatcgacag ggatatcgac agaaagatgg
    20821 aaaggacgaa gaatcgggtg ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt
    20881 acgccacctt gctgggtatt gctggcttta tgctgctgtg gtttggcgcg aatccgctgg
    20941 cctgctggct gggggtgatg ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga
    21001 aacgccactc cgtctacggc acattgattg gttcgctctc cggcgctgcg ccgccggtga
    21061 ttggctactg tgcagtaacc ggcgagttcg atagcggcgc agcgatcctg ctggctatct
    21121 tcagcctgtg gcagatgcct cactcctatg ccatcgccat tttccgcttt aaggattacc
    21181 aggcggcaaa cattccggta ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca
    21241 tcacgctgta tatcatcgcc tttgccgttg ccacgctgat gctctctctt ggcggttacg
    21301 ctggatataa atatctggtg gtcgccgcgg cggttagcgt ctggtggtta ggtatggctc
    21361 tgcgcggtta taaagttgct gatgacagaa tctgggcgcg caagctgttc ggcttctcta
    21421 tcatcgccat cactgccctc tcggtgatga tgtccgttga ttttatggtg ccggactcgc
    21481 atacgctgct ggctgctgtg tggtaacaca acctctctat taaaaaggtg ctacggcacc
    21541 ttttttctta gcattagaaa catatccctc tcgaaatatt tactaaaaaa tccgcatgtt
    21601 taccccattc gtttgccgct ttacactagt cgcgaattta aaacagaggt ggtaatgaac
    21661 gattataaaa tgacgccagg tgagcggcgc gcgacctggg gtttagggac cgtattctcg
    21721 ttgcgcatgc tgggcatgtt catggttctg ccggttctga ccacgtacgg catggctctg
    21781 caaggtgcca gcgaagcatt aatcggtatt gccattggta tttatggtct gactcaggcc
    21841 gtttttcaga ttccgtttgg cctgctttcc gaccgcattg gtcgcaaacc attaattgtc
    21901 ggtgggctgg cagtgtttgc cgccggtagc gttatcgctg cgctctctga ctccatctgg
    21961 ggaattattc tgggtcgggc gctacaaggc tccggtgcga ttgccgctgc cgttatggcg
    22021 ctgctttccg atctcacgcg cgaacaaaac cgcaccaaag caatggcgtt tatcggcgtg
    22081 agctttggca ttacctttgc cattgcgatg gtgcttggcc cgatcatcac tcacaaactt
    22141 gggctgcacg cgctgttctg gatgatcgct attctggcaa cgaccggcat tgcgttgacc
    22201 atttgggttg tgcccaacag tagcactcac gtacttaatc gtgagtccgg aatggtgaaa
    22261 ggcagtttca gtaaggtgct ggcggaaccg cggctgctga aactcaactt tggcattatg
    22321 tgtctgcata ttttgctgat gtcgacgttt gttgccctgc ccggacaact ggctgatgcg
    22381 gggttcccgg cggctgaaca ctggaaggtc tatctggcga caatgctaat cgcctttggc
    22441 tcggtcgtgc ctttcattat ctacgctgaa gttaagcgca aaatgaagca agtctttgtc
    22501 ttctgcgtcg ggttgatcgt ggttgcggaa attgtgttga ggaacgcgca aacgcagttc
    22561 tggcaactgg tggtcggcgt gcagcttttc tttgtggcgt ttaatttgat ggaagccctc
    22621 ctgccctcac ttatcagtaa agagtcgcca gcaggttaca aaggtacggc gatgggtgtt
    22681 tactccacca gccagtttct tggcgtggcg attggcggtt cgctgggcgg ctggattgac
    22741 ggcatgtttg acggtcaggg ggtatttctc gctggcgcaa tgctggccgc agtgtggctg
    22801 gcagtcgcca gtaccatgaa agaaccgccg tatgtcagca gtttgcgcat tgaaatcccg
    22861 gcgaacattg ccgcaaacga ggcgttaaaa gtgcgtttgc tggaaactga aggcatcaaa
    22921 gaagtgttga ttgcagaaga agaacattca gcttatgtga aaatcgacag caaagtgacg
    22981 aatcgctttg atgttgaaca ggcaattcgc caggcataaa agcgaggcga aattgcctgc
    23041 tgcgctacgc ttatcaggcc tactaagttc ctgatttaca aaactttgta ggccgaacca
    23101 ggcgttcatg ccgcatctga catgaacaaa gcgcaggttg tcgcgattaa tcgcggaagt
    23161 ttttgaactg gaacggctga ccgagatcgc caccgcgcac catcgccatg acagcctgta
    23221 aatcatcacg agatttgccc gtcacacgga tctcatcgcc ctgaatttgc gcctgcactt
    23281 tcagtttgct gtctttgatc atcttgacga tttttttctg agtggcgctc tcaatgccct
    23341 gtttcagttt cgcttccaca aaccaggttt taccgctatg aacgatattt tccggtacat
    23401 ccagtgaact gccttcaatg ccgcgcttca gcagcttggc acgcagaata tccagcaact
    23461 gattgacctg gaagtcggac tcgctcaaca ctttgatggt tttgctggcg tcgttcagct
    23521 caaatgaggc ttcaacgtta cggaagtcaa aacgggactc cacttcgcgg ctcgcgttat
    23581 cgaccgcgtt acgtgcttcc tgaagatcaa cttcagagac aatatcgaaa gatggcatct
    23641 tttcctctcc cttcattttt gatgcgaagc ataatacctg caaagttaaa taaccaacag
    23701 tcctgcacca ggacgtatcc cttaattgtg catagtcgca acagcatgca caattaaggg
    23761 atagccttat tgaacacccg gagtggttgc gggtgaggag gaacaatgaa aattaccgta
    23821 ttgggatgcg gtgccttagg gcaattatgg cttacagcac tttgtaaaca gggtcacgaa
    23881 gttcagggct ggctgcgcgt accgcaacct tattgtagtg tgaatctggt tgagactgat
    23941 ggttcgatat ttaacgaatc gctgaccgcc aacgatcccg attttcttgc caccagcgat
    24001 ctgctcctgg tgacgctgaa agcatggcag gtttccgatg ccgtcaaaag cctcgcgtcc
    24061 acactgcctg taactacgcc aatactgtta attcacaacg gcatgggcac catcgaagaa
    24121 ttgcaaaaca ttcagcagcc attactgatg ggcaccacca cccatgccgc ctgccgcgac
    24181 ggcaatgtca ttattcatgt ggcaaacggt atcacgcata ttggtccagc acggcaacag
    24241 gacggcgatt acagttatct ggcggatata ttgcaaaccg tgttgcctga cgttgcctgg
    24301 cataacaata ttcgcgccga gctgtggcgc aagctggcag tcaactgtgt gattaatcca
    24361 ctgactgcca tctggaattg cccgaacggt gaattacgtc atcatccgca agaaattatg
    24421 caaatatgcg aagaagtcgc ggcagtgatc gaacgcgaag ggcatcatac ttcagcggaa
    24481 gatttacgtg attacgtgat gcaggtgatt gatgccacag cggaaaatat ctcgtcgatg
    24541 ttgcaggata tccgcacgct gcgccacacc gaaatcgact atatcaatgg ttttctctta
    24601 cgccgcgccc gcgcgcatgg gattgccgta ccggaaaaca cccgcctgtt tgaaatggta
    24661 aaaagaaagg agagtgaata tgagcgcatc ggcactggtt tgcctcgccc ctggtagtga
    24721 agagactgaa gccgtcacca cgatcgacct cttgattcgc ggcggtatca aagtcaccac
    24781 tgccagcgta gccagcgatg gcaacctggc gattacctgc tcgcgcggcg tgaagctgct
    24841 ggcggatgcg ccgctggtcg aagtggctga tggcgaatat gacgtgatcg tgctgcctgg
    24901 tggcattaaa ggcgcggagt gttttcgcga tagtaccctg ctggttgaaa ccgttaaaca
    24961 gttccaccgt tccgggcgta tcgtcgcggc tatttgcgcc gcgccagcca ccgtgctggt
    25021 gccgcacgat atctttccga ttggcaatat gaccggcttc ccgacgctga aagacaaaat
    25081 tcccgctgaa caatggcagg acaagcgcgt cgtctgggat gcacgggtaa aattgctgac
    25141 cagccagggg ccgggtacag ctatcgactt tggtctgaaa attatcgacc tgctggttgg
    25201 gcgcgagaaa gcccatgaag tggcatcaca actggtgatg gcggcaggga tttataatta
    25261 ttacgagtag tgtcggatgc ggcaaacgtc gcatctgacc agatgcgacg taaaaaccac
    25321 aaattacgga cgatacacct tcacattctt aaagccctgt tcgcgtagat agagcgcctg
    25381 caggcggctc attaccccgc gctcacacca cagtagccag gttctgttct ggtcgagatc
    25441 gccaaatttg gtgctcaact tgtagaacgg cagtgaaacc acatcaatcc cttcgacttt
    25501 cagtggctta tcttcctgtt catcgacaga acggatatcg agaatcacgt cgttcgggcc
    25561 gaagtcattg acggtttcca cttccaccac ttcctgcccg gtctgctggg cgatttcgcg
    25621 gatatcaacg ttattcgctt cttcaaccac tttatcgaga atgctgaagt cgaatttctc
    25681 ttcttccgct tcaatcttcg acttaaccgc tttcaccgtc gggcttttgg agatcacacc
    25741 gcagtattcc ggcatagtac gggcaaagtc ttcggtgcca atctggcgag ccaggttgat
    25801 gatgtgctct ttgtcgtaag agatcagcgg acgcaggatc agcgtatcag agacgttatc
    25861 aatcaggcgc aggttggtca gcgtctgact ggacacctgg ccgagcgctt cgccggtgac
    25921 cagcgcctgt acgccgtaac gttcagccac tttagacgcg gcacgcacca tcatacgttt
    25981 gaggataacg cccatctgac cgtcgtcgat tttctcgaga atttccccga ctaccggctc
    26041 gaaattaata gcgacaaaac gcacgcggtg ggagctgcca aagcggttcc acagataatg
    26101 tgccacctga cgcacgccaa tttcatgcgc tgcgccgccg aggttaaaga agcagtaatg
    26161 cacgcggcag ccgcgacgca tcaacatata actggaaaca ccggagtcga aaccaccgga
    26221 aatgagcgac agcacatctt cctgggtgcc gatcgggaaa ccgccaatac cttcgtagcg
    26281 gcctttaatc agcaggagac gatcgtcttc cacttccaga tggacagtca catccggatt
    26341 ggtcagcttc acgcgcgcgg attcaatatg ctgatttaaa ccgccgccga cgtaacgttc
    26401 cacatcaatc gagctaaaat catgtttgcc acggcgcttc acgcgtacgc agaaggtttt
    26461 gccatccagc tgatcgcgat actgaaccaa cgctttctcg aaaatatcgt gcatgtcggt
    26521 aaacggcacg tcttcgactt cgagaatatg gtggatacca ggaatacggg tcagggcgtc
    26581 gcgaatagtc agacgctggt tttcatcttt cgcgcgaact tcgatgttat cccagtggcg
    26641 gacgacagcg agcgtctcat catagtgctt taaaacgtta cgaatgttcc cggtaaggat
    26701 ttttataaag cgcaagcgca cagattggct tttgatggtg atttccggga acaatttaat
    26761 gataaacttc atggcggcaa tggttcgttg gcaagcctta agcgacttgt atagggaaaa
    26821 atacagcagc ccacacctgc ggctgcatcc aggcgcggaa gtataccact aacatcgctt
    26881 tgctgcgcac atcaccttac cattgcgcgt tatttgctat ttgccctgag tccgttacca
    26941 tgacggggcg aaaaatattg agagtcagaa attcattatg ccgaagaaaa atgaggcgcc
    27001 cgccagcttt gaaaaggcgc tgagcgagct ggaacagatt gtaacccgtc tggaaagtgg
    27061 cgacctgccg ctggaagagg cgctgaacga gttcgaacgc ggcgtgcagc tggcacgtca
    27121 ggggcaggcc aaattacaac aagccgaaca gcgcgtacaa attctgctgt ctgacaacga
    27181 agacgcctct ctaacccctt ttacaccgga caatgagtaa tggactttcc gcagcaactc
    27241 gaagcctgcg ttaagcaggc caaccaggcg ctgagccgtt ttatcgcccc actgcccttt
    27301 cagaacactc ccgtggtcga aaccatgcag tatggcgcat tattaggtgg taagcgcctg
    27361 cgacctttcc tggtttatgc caccggtcat atgttcggcg ttagcacaaa cacgctggac
    27421 gcacccgctg ccgccgtaga gtgtatccac gcttactcat taattcatga tgatttaccg
    27481 gcgatggatg atgacgatct gcgtcgcggc ttgccgacct gccatgtgaa gtttggcgaa
    27541 gcgaacgcga ttctcgctgg cgacgcttta caaacgctgg cgttctcgat tttaagcgat
    27601 gctgatatgc cagaagtgtc ggatcgcgac agaatttcga tgatttctga actggcgagc
    27661 gccagcggta ttgccggaat gtgtggtggt caggcgttgg atttagacgc ggaaggcaaa
    27721 cacgtacctc tggacgcgct tgagcgtatt catcgtcata aaacaggcgc attaattcgc
    27781 gccgccgttc gccttggtgc attaagcgcc ggagataaag gacgtcgtgc tctaccagta
    27841 ctcgacaagt acgcagagag catcggcctt gccttccagg ttcaggatga catcctggat
    27901 gtggtgggag atactgcaac gttgggaaaa cgtcagggtg ccgaccagca gcttggcaaa
    27961 agtacctacc ctgcactcct gggtcttgag caagcccgga agaaagccca ggatctgatc
    28021 gacgatgccc gccagtcgct gaaacaactg gctgaacagt cgatcgatac ttcggcactg
    28081 gaagcgctag cggactacat catccagcgt aataaataaa caataagtgt taataggccc
    28141 ctgatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc cacccaggag
    28201 ctacgactgt tgccaaaaga gagtttaccg aaactctgcg acgaactgcg ccgctattta
    28261 ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac ggtcgaactg
    28321 accgtggcgc tgcactatgt ctataacacc ccgtttgacc aattgatttg ggatgtgggg
    28381 catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg caccatccgt
    28441 cagaaaggcg gtctacaccc gttcccgtgg cgcggcgaaa gcgaatatga cgtattaagc
    28501 gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc tgccgagaaa
    28561 gaaggcaaaa atcgccgcac cgtctgtgtg attggcgacg gcgcgattac cgcaggcatg
    28621 gcgtttgaag cgatgaatca cgcgggcgat attcgtcctg atatgctggt ggtcctcaac
    28681 gacaatgaaa tgtcgatttc cgaaaatgtt ggcgcgctta acaaccatct ggcgcagctg
    28741 ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt ctctggcgtt
    28801 ccgccaatta aagagctgct caaacgtacc gaagaacata ttaaaggcat ggtggtgcct
    28861 ggcacgttgt ttgaagagct gggctttaac tacatcggcc cggttgacgg tcacgatgtg
    28921 ctggggctta tcaccacgct gaagaacatg cgcgacctga aaggcccgca gttcctgcat
    28981 atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccaat caccttccac
    29041 gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg tggtttacca
    29101 agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga taacaaactg
    29161 atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc acgtaaattc
    29221 ccggatcgct attttgatgt agcgattgcc gagcaacacg cggtgacctt tgccgccggt
    29281 ctggcgattg gtggctacaa accgatcgtg gcaatttact ccaccttcct gcaacgcgcc
    29341 tatgatcagg tgctgcatga cgtggcaatt caaaaactcc cggtcctgtt cgccatcgac
    29401 cgcgcgggca ttgttggtgc tgacggtcaa acccaccagg gcgcttttga tctctcttac
    29461 ctgcgctgca taccggaaat ggtcattatg accccgagcg atgaaaacga atgtcgccag
    29521 atgctctata ccggctatca ctataacgat ggcccgtcag cggtgcgcta cccgcgtggc
    29581 aacgcggtcg gcgtggaact gacgccgctg gaaaaactac caattggcaa aggcattgtg
    29641 aagcgtcgtg gcgagaaact ggcgatcctt aactttggta cgctgatgcc agaagcggcg
    29701 aaagtcgccg aatcgctgaa cgccacgctg gtcgatatgc gttttgtgaa accgcttgat
    29761 gaaacgttaa ttctggaaat ggccgccagt catgaagcac tggttaccgt agaagaaaac
    29821 gccattatgg gcggtgcagg cagcggagtg aacgaagtgc tgatggccca tcgtaaacca
    29881 gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac tcaggaagaa
    29941 atgcgcgccg aactcggcct cgatgccgcc ggtatggaag ccaaaatcaa ggcctggctg
    30001 gcataatccc tactccactc ctgctatgct taagaaatta ttcatggcag gagtggcagc
    30061 atgcaataca accccttagg aaaaaccgac cttcgcgttt cccgactttg cctcggctgt
    30121 atgacctttg gcgagccaga tcgtggtaat cacgcatgga cactgccgga agagagcagc
    30181 cgtcccatca ttaaacgcgc actggaaggc ggcataaatt tctttgatac cgccaatagc
    30241 tattccgatg gcagcagcga agagatcgtc ggtcgcgcac tgcgggattt ctcccgtcgt
    30301 gaagacgtgg tcgttgccac caaagtgttc catcgcgttg gtgatttacc ggaaggatta
    30361 tcccgtgcac aaattttgcg ctctatcgac gacagcctgc gacgtctcgg catggattat
    30421 gtcgatatcc tgcaaattca tcgctgggat tacaacacgc cgatcgaaga gacgctggaa
    30481 gccctcaacg acgtggtaaa agccgggaaa gcgcgttata tcggcgcgtc atccatgcac
    30541 gcttcgcagt ttgctcaggc actggaacta caaaaacagc acggctgggc gcagtttgtc
    30601 agtatgcagg atcactacaa tctgatttat cgcgaagaag agcgcgagat gctgccgctg
    30661 tgttatcagg aaggcgtggc ggtcattccg tggagtccgc tggcgcgggg gcgactgacg
    30721 cgtccgtggg gagaaactac cgcacgactg gtgtctgatg aggtggggaa aaatctctat
    30781 aaagaaagcg atgaaaatga cgcgcagatc gcagagcggt taacgggcgt cagtgaagaa
    30841 ctcggtgcaa cacgagcaca agttgcgctg gcctggttgt tgagtaaacc gggcattgcc
    30901 gcaccgatta tcggaacttc gcgggaagaa cagcttgatg agctgctgaa cgcggtggat
    30961 atcactttga agccggaaca gattgctgaa ctggaaacac cgtataaacc gcatccggtc
    31021 gtaggattta agtaaatttc gcttttcacc cattttatct gctgaattcc ccatcgtctg
    31081 cgatgatggg gacaacgtac ctcccatcac cttctgtaac cataatccac ctctgcaaga
    31141 gaaattcttc gtgaatgagt ttgcagctat tcatcgtctt catccctttg agcgattttt
    31201 gatagtttct ccaggtaata tcccctaaaa ctgtcttatt tagtcaacgt aagaggatta
    31261 caatcatgat gaaaaccatc accaaacaac cgatcctgtt tactgatgta ccggttgcag
    31321 atttaagaaa ctcaatgaag caagatttaa atcaaaatct tattgaaagg ctgtggaaca
    31381 aa
//
LOCUS       DBFIFS010000036        30058 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.036, whole genome shotgun sequence.
ACCESSION   DBFIFS010000036 DBFIFS010000000
VERSION     DBFIFS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.036"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1546
                     /gene="mltF"
                     /locus_tag="N7A07_001496"
     CDS             <1..1546
                     /gene="mltF"
                     /locus_tag="N7A07_001496"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU2476185.1"
                     /translation="KINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVS
                     TIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLA
                     AGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDL
                     QTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALD
                     ITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTF
                     LRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTA
                     QSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQDARA
                     LTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGY
                     LQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKG
                     SEEKQN"
     gene            complement(1543..2046)
                     /gene="tadA"
                     /locus_tag="N7A07_001497"
     CDS             complement(1543..2046)
                     /gene="tadA"
                     /locus_tag="N7A07_001497"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU2476186.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2104..2739)
                     /gene="yfhb"
                     /locus_tag="N7A07_001498"
     CDS             complement(2104..2739)
                     /gene="yfhb"
                     /locus_tag="N7A07_001498"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU2476187.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2948..3796
                     /gene="yfhH"
                     /locus_tag="N7A07_001499"
     CDS             2948..3796
                     /gene="yfhH"
                     /locus_tag="N7A07_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU2476188.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3852..4112
                     /gene="yfhL"
                     /locus_tag="N7A07_001500"
     CDS             3852..4112
                     /gene="yfhL"
                     /locus_tag="N7A07_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU2476189.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(4306..4386)
                     /gene="shoB"
                     /locus_tag="N7A07_001501"
     CDS             complement(4306..4386)
                     /gene="shoB"
                     /locus_tag="N7A07_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU2476190.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4808..5188)
                     /gene="acpS"
                     /locus_tag="N7A07_001502"
     CDS             complement(4808..5188)
                     /gene="acpS"
                     /locus_tag="N7A07_001502"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU2476191.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(5188..5919)
                     /gene="pdxJ"
                     /locus_tag="N7A07_001503"
     CDS             complement(5188..5919)
                     /gene="pdxJ"
                     /locus_tag="N7A07_001503"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU2476192.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5931..6659)
                     /gene="recO"
                     /locus_tag="N7A07_001504"
     CDS             complement(5931..6659)
                     /gene="recO"
                     /locus_tag="N7A07_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU2476193.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6671..7576)
                     /gene="era"
                     /locus_tag="N7A07_001505"
     CDS             complement(6671..7576)
                     /gene="era"
                     /locus_tag="N7A07_001505"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU2476194.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7573..8253)
                     /gene="rnc"
                     /locus_tag="N7A07_001506"
     CDS             complement(7573..8253)
                     /gene="rnc"
                     /locus_tag="N7A07_001506"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU2476195.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8526..9500)
                     /gene="lepB"
                     /locus_tag="N7A07_001507"
     CDS             complement(8526..9500)
                     /gene="lepB"
                     /locus_tag="N7A07_001507"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU2476196.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(9516..11315)
                     /gene="lepA"
                     /locus_tag="N7A07_001508"
     CDS             complement(9516..11315)
                     /gene="lepA"
                     /locus_tag="N7A07_001508"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU2476197.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(11513..11992)
                     /gene="rseC"
                     /locus_tag="N7A07_001509"
     CDS             complement(11513..11992)
                     /gene="rseC"
                     /locus_tag="N7A07_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU2476198.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11989..12945)
                     /gene="rseB"
                     /locus_tag="N7A07_001510"
     CDS             complement(11989..12945)
                     /gene="rseB"
                     /locus_tag="N7A07_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU2476199.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12945..13583)
                     /gene="rseA"
                     /locus_tag="N7A07_001511"
     CDS             complement(12945..13583)
                     /gene="rseA"
                     /locus_tag="N7A07_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU2476200.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13616..14191)
                     /gene="rpoE"
                     /locus_tag="N7A07_001512"
     CDS             complement(13616..14191)
                     /gene="rpoE"
                     /locus_tag="N7A07_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU2476201.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(14188..14343)
                     /gene="rseD"
                     /locus_tag="N7A07_001513"
     CDS             complement(14188..14343)
                     /gene="rseD"
                     /locus_tag="N7A07_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU2476202.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14599..16221
                     /gene="nadB"
                     /locus_tag="N7A07_001514"
     CDS             14599..16221
                     /gene="nadB"
                     /locus_tag="N7A07_001514"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU2476203.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(16206..16943)
                     /gene="trmN"
                     /locus_tag="N7A07_001515"
     CDS             complement(16206..16943)
                     /gene="trmN"
                     /locus_tag="N7A07_001515"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU2476204.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKAGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            17075..18409
                     /gene="srmB"
                     /locus_tag="N7A07_001516"
     CDS             17075..18409
                     /gene="srmB"
                     /locus_tag="N7A07_001516"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU2476205.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(18442..19323)
                     /gene="yfiE"
                     /locus_tag="N7A07_001517"
     CDS             complement(18442..19323)
                     /gene="yfiE"
                     /locus_tag="N7A07_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2476206.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            19426..20013
                     /gene="eamB"
                     /locus_tag="N7A07_001518"
     CDS             19426..20013
                     /gene="eamB"
                     /locus_tag="N7A07_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU2476207.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(20069..20452)
                     /gene="grcA"
                     /locus_tag="N7A07_001519"
     CDS             complement(20069..20452)
                     /gene="grcA"
                     /locus_tag="N7A07_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU2476208.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            20757..21446
                     /gene="ung"
                     /locus_tag="N7A07_001520"
     CDS             20757..21446
                     /gene="ung"
                     /locus_tag="N7A07_001520"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU2476209.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(21494..22531)
                     /gene="yfiF"
                     /locus_tag="N7A07_001521"
     CDS             complement(21494..22531)
                     /gene="yfiF"
                     /locus_tag="N7A07_001521"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU2476210.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            22738..23157
                     /gene="trxC"
                     /locus_tag="N7A07_001522"
     CDS             22738..23157
                     /gene="trxC"
                     /locus_tag="N7A07_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU2476211.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            23226..23924
                     /gene="tapT"
                     /locus_tag="N7A07_001523"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             23226..23924
                     /gene="tapT"
                     /locus_tag="N7A07_001523"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU2476212.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            23956..26616
                     /gene="pat"
                     /locus_tag="N7A07_001524"
     CDS             23956..26616
                     /gene="pat"
                     /locus_tag="N7A07_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU2476213.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALLGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            26730..28085
                     /gene="pssA"
                     /locus_tag="N7A07_001525"
     CDS             26730..28085
                     /gene="pssA"
                     /locus_tag="N7A07_001525"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU2476214.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLLDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            28131..28454
                     /gene="yfiM"
                     /locus_tag="N7A07_001526"
     CDS             28131..28454
                     /gene="yfiM"
                     /locus_tag="N7A07_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU2476215.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(28451..29749)
                     /gene="kgtP"
                     /locus_tag="N7A07_001527"
     CDS             complement(28451..29749)
                     /gene="kgtP"
                     /locus_tag="N7A07_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU2476216.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(29866..>30058)
                     /locus_tag="N7A07_001528"
     CDS             complement(29866..>30058)
                     /locus_tag="N7A07_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476217.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT         7351 a         7900 c         7501 g         7306 t
ORIGIN      
        1 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
       61 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
      121 gttgcgtgtg agcaccattc atactcccct gacgtataac gaaatcaacg ggaaaccttt
      181 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
      241 gaccgtgcgg cagaatatca gccagctgtt tgacgacctc gataatggta acgccgacct
      301 gctggcggca ggacttgtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
      361 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
      421 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtgg ttaacgatct
      481 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
      541 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
      601 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
      661 cgatgaacaa ccggtgacct ggtttagccc gttagatggc gataataccc tttccgccgc
      721 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
      781 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
      841 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
      901 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
      961 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
     1021 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
     1081 gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc tggtttgccc tcgctgcgta
     1141 caatatgggc tatgcgcata tgcaggatgc ccgcgccctg acggcaaaaa ccaaagggaa
     1201 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
     1261 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
     1321 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
     1381 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
     1441 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
     1501 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
     1561 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
     1621 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
     1681 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
     1741 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
     1801 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccgc cctgcctcag
     1861 ggccatgatt tctgcatgtg cggtgggatc atgtcgacca atcggacggt tccagccttc
     1921 gccaataaca cgattgttat gcactaatac cgcgccgacc ggtacttccc gctcatccca
     1981 ggcacgcttc gccagcgtca ttgcatgacg catccagtat tcgtggctaa attcgacttc
     2041 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
     2101 actctattcc agttgctgga gttcaccgcg cggggttact cgccagcgat gctggcaaaa
     2161 gtaaagcagc gggttgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
     2221 gccgattttg cgctccagtt gcgcgacctt ttcatgcccg aggcaacgca tcgtcaatac
     2281 ccaaccacca tagccacgct gaatttggct ggcgataaga ttcacccgcg gcagccaggg
     2341 tgtatcgaaa tagaccgctt caaccagcgg ctgtggagaa ccggtaatca accagatatc
     2401 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
     2461 attgtcacga aaccagcgta cgaaatcggc ctgcaacgcc tgtaaacgtg cttcgctgtg
     2521 accaaaggtg catccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
     2581 caataacgca atggctataa tcggcaacaa cggcaggaca agtaacgcat tcagcggttg
     2641 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
     2701 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
     2761 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
     2821 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
     2881 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
     2941 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg cccagagtga
     3001 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
     3061 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
     3121 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
     3181 ggaatcgccc tcagtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
     3241 cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa acgttaatag
     3301 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
     3361 cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg
     3421 cttcaatgct gccgcagtgc gcgatatgca tgcgctactc gctaccgtac aggcgtcgtc
     3481 ccctgacgat ctgttattag ccatttctta caccggtgta cgacgcgagt taaacctggc
     3541 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactcccaa
     3601 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
     3661 cagtgcttca atctctgctt gtcacgcgca gggaatgtta acggatttgc tgttcattgc
     3721 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
     3781 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
     3841 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaac
     3901 ccgaatgccc gaatgaggcg atttcaatgg gagatcatat ctatgagatt aacagcgata
     3961 agtgtaccga atgcgtaggg cactacgaga caccaacctg ccagaaggtg tgcccgatcc
     4021 ccaatactat tgtgaaagat ccgcaacatg tcgagacaga agaacagttg tgggataaat
     4081 ttgtgctgat gcaccacgcg gataaaattt aatattctac tctggaagta gagtattaat
     4141 tattttactg ggaagcctta acgccattat atttatttaa ttgatgacat tagcataata
     4201 attcactaag ttaatttata tagtatctgc ccagacactt atttatagtt attaaaggcg
     4261 cgtccgattg gttcaccgga cgcaccttaa gtacgtttcc ttgtgttata agaacagaag
     4321 gatcagctgt aaaacagcaa tgatgatttt gatgacccgt ttaatcaggt atcggcaatc
     4381 agtcattcgt ttttccttaa acaaggaatg cagccgttca gattagccct tacatctccc
     4441 caaaactgaa cgtgcgagtt attggaatcg agtgctgcac tccacaccag agctttgacg
     4501 acaccactcg tttcaatggg ggaattcagt ggcatggtgt aaagcacagc aaaattgcca
     4561 ataacgaaac cgattttaat gtacttatca aaaagcggtc acgttaatct tttcatcaat
     4621 gtcgatttct tcgtaaaggc tcgcgttttt tatgtcagag attgcaagtt gcttgtacag
     4681 gtcaacgaaa ttgatccata atcattttcg ttggaatcgt gtgctgcaca aaattaaagt
     4741 taaaaagtaa aacccccgtt ccttaccagt tcgggggttt tactttataa agagaacggt
     4801 attattttta actttcaata attaccgtgg cacaagcata gtggcgctca tcggccagcg
     4861 tcacatgcat atttgcaacg cccagctttt ccgccagttt taatgcctcg ccccacagtc
     4921 gcagccgtgg cttaccaagc tcatcgttaa atacttcaaa ctgattaaac gccagaccat
     4981 tgcggatccc ggtgccaaac gcttttgctg cggcttcttt tacggcaaag cgtttcgcca
     5041 gaaaacgcac cggctggtgg tgcgttttcc aaatttccca ttcgttatcg cttaatacgc
     5101 ggcgtgccag gcgctcaccg gatcgggcga tcactgcttc tatgcgagcg atctctacga
     5161 tatccgtgcc taaacctaat attgccatta gccacgcgct tccagcatca ggcgcttcat
     5221 ttctgccacc gcatctttca gtccggtcat cactgcacga ccaataatgg catgaccgat
     5281 attcagttcg tgcatctcag ggatggcggc aatggctttt acgttgtgat aggtcagacc
     5341 gtgtccggcg ttaactttca aaccgagact agcggcaaag gtcgcggctt tggcgatacg
     5401 cgccagctct tgcgcctgtt cggcgtcagt tttggcatca gcatagcaac cggtgtggat
     5461 ctcgataaac ggtgcgccaa cctctgccgc agctttgatc tgctcttcat cggcgtcgat
     5521 aaacagagaa acctgaatcc cggcatccgc cagacgtttg caggcatcgc gcattttgtc
     5581 acgctgccct gcaacgtcca ggccgccttc ggttgttact tcctgacgct tttctggtac
     5641 caggcagcaa aaatgcggct tcgtctcaac cgcgatcgcc agcatctctt cggtcaccgc
     5701 catctccaga ttcatgcggg tatccagcgt ctgacgcagg atgcgcacat cgcggtcagt
     5761 aatgtggcgg cgatcttcac gtaaatgcac ggtaatgccg tccgcccccg cctgctcggc
     5821 aataaacgcg gcctgcaccg gatccgggta agcggtaccg cgcgcgttgc gcagcgtagc
     5881 gatatggtca atgttgacgc ctaacagtaa ttcagccatg acaatcctca tcattcataa
     5941 tgtgttttca ccgttcgctt aggcataaat tgccggaaca gttccctgct ctttaaaggt
     6001 ttaccgccaa gatacggctt aagcgccatg cgggtaaagc gtttcgcggc gcgcagtgtg
     6061 tctgcgtcag gaaattcccg tgcgtttaac gcttttaact gccttccggt gaacgtttta
     6121 ttgtcgataa cgacgcttgc gataaaccct ttttcttcgc gataacgata cgtcatggtg
     6181 tcatctaccg gctcgccgct acccgcacaa tgggtaaaat tgacgccata acccagatgc
     6241 ccgagcagtg ccagttcaaa gcggcgcagc gcgggttctg gcgtaccagt gacccctgca
     6301 agagactgaa tgcagtgcaa gtaatcgaaa aagagttcag agaagcgcgt ctcgtattcc
     6361 agtacgcggg agagaagttc gttgatgtac agaccgctgt aaagcgtgat accgcttaat
     6421 ggcagcgcca gcgagacagc ttcagcactg cgcagcgttt tgacttcgcc acgcccgcca
     6481 aaacgtagca agagaggggt gaaaggctgt aatgcacctt tcagggtaga gcgtttagag
     6541 cgtgcgcctt tggcaaccag acgcacgcga cccgattcct ccgtgaagac gtccagcatc
     6601 aggctggttt cgctccacgg gcgactatgc aggacaaatg cgcgctgcca gccttccatc
     6661 ggagttactc ttaaagatcg tcaacgtaac cgagactgcg cagtgcgcgt tcgtcgtcgg
     6721 cccaaccgga tttcactttt acccacagct caaggtgaac aggcgcttca aacatttcct
     6781 gcatgtcttt acgcgcttca atcccgatgg ttttgatctt ggcccctttg ttgccaatga
     6841 ccatcttctt ctgcccttca cgctcaacga gaatcaaacc gttgatgtca taaccaccgc
     6901 gttcgttaga gacgaaacgt tcgatctcca cggtcacgga gtacggcagt tcagcgccga
     6961 ggaaacgcat cagtttttcg cggatgattt cagacgccat aaaacgctgt gagcgatcgg
     7021 tgatgtaatc ttccgggaag tgatgagtcg cttcaggtag atgcttacgc acgattgccg
     7081 caatagtggc aacattcagc ccggtttcgg cagagattgg cacgatatcg aggaagttca
     7141 tctggcttgc caggaactgc aggtgcggca gcagatcggc tttctcctgc acgttgtcca
     7201 ctttgttcac cgcgaggatt accggcgctt tgccttcgcg cagtttgttg agcaccattt
     7261 cgtcgtccgg cgtccagcgg gtgccttcaa cgacaaaaat caccagctca acatcgccaa
     7321 tagagctgct cgccgctttg ttcatcaggc ggttaatagc gcgtttttct tccatatgca
     7381 ggcccggtgt atcgacgtag atcgcctgat acgcgccttc agtatggatc cccacaatgc
     7441 ggtgacgagt tgtctgcgcc ttgcgggagg tgatggagat tttctgcccc agcagtttgt
     7501 tcaacaatgt ggatttgcca acattcggac gtccgacgat ggcaataaat ccgcagtaac
     7561 ttttatcgat gctcattcca gctccagttt tttcaacgcc tgttcggcgg cagcctgctc
     7621 agccttacga cggcttgaac ctgtgccaac caccggttca ctcaggccgc tgacctggca
     7681 gtggattgta aattcctgat cgtgcgcttc gccacggacc tgaactacca gataagtcgg
     7741 cagcggcaga tgacgaccct gcaaatattc ttgcaagcgc gttttcggat ctttttgttt
     7801 atcgcctggg ctaatttcgt ccagacgagt ttgataccag ttgaggatta atttctcgac
     7861 ggtttgaata tcactgtcga ggaatacgcc accaattaat gcttcgacgg tgtcggcgag
     7921 aattgactca cgacgaaatc caccgctttt aagttcacct ggtcctaaac gtaagcactc
     7981 gcctaactca aattcgcgcg ccagttccgc cagcgtattg ccacggacca gcgtggcgcg
     8041 catccggctc atatcgcctt catccacacg agggaaacgg tgataaagcg cattggcgat
     8101 aacgtagctc agaatagagt cgcctaaaaa ttctaaacgc tcgttatgtt tgctgctggc
     8161 actacgatga gttaatgcct gctgcaacag ttcctgatga ttaaaagtgt agcccagctt
     8221 ccgttgaagc cgattaatta cgatggggtt catgcgatac caataaataa atgcgtcaac
     8281 aattcagcac acgaaacaga cctgatatac atggctctgc taactgcttc gctgcagttt
     8341 cagtttagcg gtatatgggg accaacgctg tttcgtgtgc cgtggcaacc tggaggtgcc
     8401 aaccttaaac ttcgggggaa tattctatac acaacgacgg gggatgtcgt tagccacggg
     8461 agatttatct cataaataat tcacgttgtc gccataacgg cgacaacgtg aacgaagatg
     8521 agctattaat ggatgccgcc aatgcgactt aagcgcacac ctgtcggcca ttcgccttct
     8581 tgcttatcga agctcatcca gatagccgtt gcccgaccga ccagattcgc ttcaggcaca
     8641 aagccccagt aacggctgtc cgcgctgttg tcgcggttgt cgcccatcat gaagtattgt
     8701 cccggaggaa caatccaggt tgccagttgt tgccctggct gctggtaata catccccacc
     8761 tgatcctgcg caatcggcac tgtcagaatg cggtgcgtca catcacccag tgtctcttta
     8821 cgctcggaaa gacgaattcc attttctttg gtttcgtttt tcggcacttc aaagaatccg
     8881 ctggtcgctt ccccaccatt acggcgtgag aaggtctgaa cgaaatcgct cggttccacg
     8941 tttgagtagg tgaccggcag cgcgttttca cacgcctggc cggaactgca tcccggttga
     9001 atcgtcagct cttttgagac cggatcgtaa gtgactttat cgcccggtaa acccaccgcg
     9061 cgcttgatat aatcaagctt tggatcttcc ggatatttaa agaccacgat atcgccgcgt
     9121 ttcggatgac cggtttcgat cagcgttttc tggtagatag gatctttaat gccataagca
     9181 aacttctcta ccagaataaa atcaccgatt aacagagtcg gcatcatcga acctgacgga
     9241 atctggaacg gttcataaat aaacgaacgc acaatcaata cgatagccag taccggaaaa
     9301 acagaagcac cggtttccag ccagccaggc ttcggcgcaa cctttttcaa cgttgcttta
     9361 tccagtgagt caccggcagc cgcctgcgcc gctgcctgac gttcccgccg tttaggtgcg
     9421 aaaaagaatt tatccacgca ccataaaatg cccgtcacca gtgtggcaat caccagaatc
     9481 agggcaaaca tattcgccat gccaactcct aagggttatt tgttgtcttt gccgacgtgc
     9541 agaatggcga ggaacgcttc ctgcggcagc tcgacgttac cgatctgctt catgcgtttc
     9601 ttaccttctt tctgcttctg cagcagcttt ttcttacggc tgatatcgcc gccataacat
     9661 ttagccagta cgtttttacg cagctgtttc acggtggatc gcgcaataat gtgcgtacca
     9721 atcgctgcct gaatggcgat atcaaactgc tggcgtggga tcagatcttt catcttctcc
     9781 accaactcgc gaccgcggtt ttgcgaatta tcacggtggg tgatcaacgc cagcgcatca
     9841 acacgttcac cgttgattaa tacgtctaca cgtaccatgt cggacgcctg gaagcgtttg
     9901 aagttataat ccagagacgc ataaccacgc gaggtagatt tcaggcgatc gaagaagtcg
     9961 agcaccactt ccgccatcgg gatctcgtac gtcagcgcca cctgattacc gtggtaaacc
    10021 atattggtct gcacgccgcg tttttctacg cacagcgtaa taacgttgcc gagatatgcc
    10081 tgcggcagca gcatgtgaca ctctgcaatc ggctcgcgca gttcgtaaat gttatttacc
    10141 gcaggcagct tggatgggct gtcgacgtag ataacttccc ttgacgtggt ttcaacttca
    10201 tacactacgg tcggcgcagt ggtgatcaga tccagatcgt attcacgttc cagacgctcc
    10261 tggatgatct ccatgtgcag caggccaagg aagccgcagc ggaaaccaaa gcccagcgcg
    10321 ctggagcttt ccggctcata gaacagtgag gcatcgttca ggctgagttt acccagcgcg
    10381 tcacggaagg cttcatagtc gtcggaactt accgggaaca gaccggcgta tacctgcggt
    10441 ttgactttct taaagccagg cagcgccttt tctgccggat tacgcgccag cgttaaggta
    10501 tcgccgactg gagcgccgtg gatatcttta atcgcacata cgagccagcc tacttcgcca
    10561 catttcagtt cagtgcggtc aacctgtttc ggcgtgaaga tgcccagacg gtcggcgtta
    10621 taggtctgcc cggtactcat gactttcact ttgtcgccct tacgcagggt gccgttttta
    10681 atacggataa gtgaaacaac gcccaggtag ttgtcgaacc atgagtcgat aattagtgcc
    10741 tgcaacgggc cttccggatc gccttccggc ggcggaatgt cgcgcaccag acgttcgaga
    10801 acgtcctgca cgccaacgcc ggttttcgct gaacagcgca ccgcgtcagt ggcgtcgatg
    10861 ccgacgatat cttcaatttc ttccgccacg cgttcaggat cggctgccgg caggtcaatc
    10921 ttgttcagta ccggcaccac ttcgagatcc atttccatgg cggtgtagca gtttgccagg
    10981 gtttgcgctt ccacgccctg cccggcgtcg accaccagca atgcaccttc acaggcagcc
    11041 agcgaacggg aaacttcata ggagaagtct acgtggcccg gggtgtcgat aaagttaagc
    11101 tgataggttt cgccgtcaga cgctttgtag tccagcgtca cgctttgcgc tttgatggta
    11161 atgccacgct cacgctcaag atccatggaa tcgagaacct gcgcctccat ttcacggtca
    11221 gacaggccac cgcagatctg gataatacgg tcagacagcg tcgatttacc gtggtcaatg
    11281 tgagctatga tcgaaaagtt acgtatattc ttcataaagt atgattattg tgccttaatg
    11341 cccggttaac caggctttta gaagtcgctg ttctgagctt aacgtctgta ttaatagaaa
    11401 cgccgcattc tacactacaa cattgaggcg aggaaatgtt cataccgtat ggattgtggt
    11461 atctggaaac gtcctcgcat ttgttatgca aaatgcaaca aagccagtga aatcactggc
    11521 tcgcgtcttc cgaagatgtt tcaaatcgca caaggccagg cggcaatgcc acgcttaaga
    11581 tgatcggttg ccattctgcc cgggcagcaa acttacgcga gtagccacgc gcaatcagga
    11641 acccgccaat accgccgagg accgcaccac ataatgctgc gacgtcagaa gcaaagagta
    11701 gctgaaatag cgaagcgatg aggaataatc ccaccagcgg cgacatataa accagtaatg
    11761 cggagctaag caggctgcct tcggcgatcc ccaattccac tttttgcccc ggcactaacg
    11821 gctcatcaca gggtacgaca atggtatgcg tggtttgcgg gccaagttta ttcaacacgc
    11881 ggctaccgca accggcgcgt gaagcgcagc tgctgcatga ggctttaaca tcacaactga
    11941 ccagcgcctg cccgttttgc caggagacga cggtagccca ctctttgatc attgcgctgc
    12001 cccgaactta atattctcgg caatgcgttt cgccgtttgc ggcggcagtt caccgacaat
    12061 ggtgatttcg gcgttatcac gtacgcttgt actgacggtt ctgcgtccgg tgcgcaacat
    12121 ctgatcggtg ctcgatggcg tagcgcggtt aacgtttacc gagaagctga ataatccgtc
    12181 ggaatagaga cgtgattcga taggcatgtt gtccatcgtc ggtagcggac gtcgactact
    12241 ggaaacttcg ctaaaaccct gtggcaacca ggttggcgtc cagctgaatt tagctttttc
    12301 acctacagga acagaaagca acggcggcaa atttgccttc gccagcgtct gcatactgct
    12361 gctgatatcc tgattgacgt taaaagcaat cacgcgaaat tgttccagcg tttcaccatc
    12421 gcgatcaaga agatcaaccc gcatcggtaa tttcgattcg gtgtccatcc acacgatgta
    12481 gctgtagcgt gtaccatctc gggcaaccac gcgaatgact tcgcaaagac gatcagcaat
    12541 acgcgtgcgc ccgacggaga taaagtcgta gtaaggagaa aggcgtttga aatcggtata
    12601 gatgagcgat ggcagagaat caacaatgta atcgccatta agcgtgaacg gttcaagtcc
    12661 cggttcaaaa tagctgattt cattgccgcg ctgtaccact tcccggcgcg ggccatccat
    12721 ttgcaacaat tgtgcaagag gacggttatc gaggcgtgca tgtcgataac gcagagactc
    12781 aacaccctgt ttattgatgc tgatgaatga cagctcgtaa ttcagtgact gactggccag
    12841 gttcatctgc tgtaataacg ccccggacgc gggagtggcc gaggcgttag cagagaataa
    12901 caggctacct gtcactaatg acatggcaaa ccaaagttgc ttcattactg cgattgcgtt
    12961 cctaaagttt gaattcctgg cacctgtaca gcggcttgct gggtttgcgc ctgctcaaac
    13021 tgaagctgtt cagagtggag tcggcgttgc agttcgtaat cctgcaacat tgcattaatg
    13081 cgacgacgct gctgctgttg accattattt gcggtcgctt cagaaggtac tcccaggcta
    13141 accgggctgg ctttacccat catcggcagt gtattaaata ccggcgtttc gggctgctgg
    13201 gacgtttcag attgtccatt atagtgctgg acgccaacga taactgcaag cgatacgcat
    13261 gcggctacgc ccatttgggt aagctgtgcc gcccacggac gtactttctg ccagaatggc
    13321 attttctgcc attcatgcgg cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt
    13381 actggctctt cttcaatggc ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg
    13441 ggagtatcac cccgcattga gtcacggatt aagtgatagc tttcccaggt tttctgcatt
    13501 tctgggttat gagccagttc gttaagcagc tcactatcca gcgtttcgcc atccattaaa
    13561 gcggaaagtt gttctttctg catgcctaat acccttatcc agtatcccgc tatcgtcaac
    13621 gcctgataag cggttgaact ttgttatcaa tagcttccct cgctcggaag atacgtgaac
    13681 gcaccgtacc taccggacaa tccatgatag cggctatctc ttcatagctc aggccatcca
    13741 gctcccgcaa ggttattgcc atgcgtaaat cttccgggag ggactcaata gttcggaaaa
    13801 ctatctgtct cagttcttct gacaacatta agttctcagg gttcgaaatt tctttcaacg
    13861 cgccgccact ttcgaagttt tcagcttcaa tggcatccac atcactggaa ggtggacgac
    13921 gcccctgagc aaccaggtaa tttttcgctg tatttacagc aatccgatac agccatgtat
    13981 aaaaagcgct atctccccgg aacgaatcca gcgcacgata ggctttaata aaagcttctt
    14041 gtaccacatc gggaacatca cccgacggca catagcggga aaccagactc gccactttat
    14101 gctgatagcg cactaccagt aagttaaagg ctttctgatc tcccttctgg acccgttcaa
    14161 ccaggacctg gtccgttaac tgctcgctca tccgaggtaa agtctcccca aaccaaattt
    14221 ccacgcgcta tcgaaacgcc actccattag ctgcaatttg agcaagcaaa gggttagagt
    14281 gtctcgtttt tgtaaagttc cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat
    14341 cattatctat cattataagt ctacagaatc tgaacatcgc attatctgtg tagaaatgcc
    14401 catttaactg cctgaagagt aacccaacgg cctttttatt tcaccaccta atcctccacc
    14461 agccagtaac ttctcttttt ctcgccgccc tgcgtcagcg tgtttagtca ctgtaacaaa
    14521 tattaaaata gcaggtgttt attcgcacaa catgatgcta tgctgaccaa accgtgttta
    14581 gtaaattaaa caaagaaaat gaatactctc cttgaacatt catgtgacgt gttgattatc
    14641 ggtagcggcg cagccgggct ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc
    14701 gttctaagta aaggcccggt aacggaaggt tcaacatttt atgcccaggg cggtattgcc
    14761 gccgtgtttg atgaaactga cagcattgac tcgcatgtgg aagacacatt gattgccggg
    14821 gctggtattt gcgatcgcca tgcagttgaa tttgtcgcca gcaatgcacg atcctgtgtg
    14881 caatggctaa tcgaccaggg ggtgttattt gatacccaca ttcagccgaa tggcgaagaa
    14941 agctaccatc tgacccgtga aggtggacat agtcaccgtc gtattcttca tgccgccgac
    15001 gcaactggta gagaagtaga aaccacgctg gtgagcaagg cgctgaacca tccgaatatt
    15061 cgcgtgctgg agcgcagcaa cgcggttgat ctgattattt ctgacaaaat tggcctgccg
    15121 ggcacgcgac gggttgttgg cgcgtgggta tggaaccgaa ataaagaaaa ggtggaaacc
    15181 tgccacgcaa aagcggtggt gctggcaacc ggcggtgcgt cgaaggttta tcagtacacc
    15241 accaatccgg atatttcttc tggcgatggc attgctatgg cgtggcgcgc aggctgccgg
    15301 gttgccaatc tcgaatttaa tcagttccac cctaccgcgc tgtatcaccc acaggcacgc
    15361 aatttcctgt taacggaagc actgcgcggc gaaggcgctt atctcaagcg cccggatggc
    15421 acgcgtttta tgcccgattt tgatgtgcgc ggcgaactgg ccccgcgcga tattgtcgcc
    15481 cgcgccattg accatgaaat gaaacgcctc ggcgcagatt gtatgttcct tgatatcagc
    15541 cataagcccg ccgattttat tcgccagcat tttccgatga tttatgaaaa gctgctcggg
    15601 ctggggattg atctcacaca agaaccggta ccgattgtgc ctgctgcaca ttatacctgc
    15661 ggtggtgtaa tggttgatga tcatgggcgt acggacgtcg agggcttgta tgccattggc
    15721 gaggtgagtt ataccggctt acacggcgct aaccgcatgg cctcgaattc attgctggag
    15781 tgtctggtct atggctggtc ggcggcggaa gatatcacca gacgtatgcc ttatgcccac
    15841 gacatcagta cgttaccgcc gtgggatgaa agccgcgttg agaaccctga cgaacgggta
    15901 ataattcagc ataactggca cgagctacgt ctgtttatgt gggattacgt tggcattgtg
    15961 cgcacaacga agcgcctgga acgcgccctg cggcggataa ccatgctcca acaagaaata
    16021 gacgaatatt acgcccattt ccgcgtctca aataatttgc tggagctgcg taatctggta
    16081 caggttgccg agttgattgt tcgctgtgca atgatgcgta aagagagtcg ggggttgcat
    16141 ttcacgctgg attatccgga actgctcacc cattccggtc cgtcgatcct ttctcccggc
    16201 aatcattaca taaacagata aaaagcctgg gttagcgccg tatacgcttc ggaatagttc
    16261 tggtctggcc cacgaatgac taagcgatcg ctaaagcatt ctcccgcctg cggggagaat
    16321 gccagcagca cccgatgcgg cagtcgcgct tcgttttccg ccacatccgt ccgcaaacgt
    16381 aaatgccagc ccatacttaa tgccagctcc gtaaaactat taccaatctg ctctggcagc
    16441 accacgcaga aaaatccctc ttcggtaatg cactccgccg cgcaagtaag taaagtctgg
    16501 tgatcaagtg aggtggtgta gcgcgcctgt tcccgctgcg gcgtcgcgca ctccactcct
    16561 tgctcataat acggcgggtt gctgatgatt aaatcaaagc gcgcggtctg ctgtgttacc
    16621 cactgctgaa tatccgccgt atggacgtta attctctcta accacgggga ctgggcgaca
    16681 ttttcctgcg cctgagtggc agcttcactt tccagctcaa cggcatcaat tatcacgctg
    16741 tcactggttc gctgcgccag catcagtgcc agcaagccac tacccgcacc gatatcaagg
    16801 caacgtttta ccccagccac cggtgcccat gcgcccagca atatgccatc cgttcccgct
    16861 ttcatcgcac agcgatcgtg agcaacaaaa aactgtttaa aagtaaatcc attacgacga
    16921 agcacggatg tagactgtga catgaaaata aaaccttgca ggaaaaacgg cgatagcacc
    16981 gggtgagaac aatacccgag aagcgatatc catacaaaca gatgaagatt gcagccgtaa
    17041 cgtctataat cagcgcccca cacagaggta gaacatgact gtaacgactt tttccgaact
    17101 tgaactcgac gaaagcctgc tggaagccct ccaggataaa ggtttcactc gcccgaccgc
    17161 cattcaggct gccgccattc cgcctgcgct cgatggccgt gatgtactcg gttctgcgcc
    17221 gacaggcacc ggtaaaacgg cggcgtatct gctgccagcg ttgcagcacc tgctcgattt
    17281 cccgcgtaag aaatccggtc cgccgcgtat tttgatcctc accccaactc gcgagctggc
    17341 gatgcaggtg gccgatcatg cccgcgaact ggcgaaacat acgcatctgg atatcgccac
    17401 catcaccggc ggcgtagcct atatgaacca cgcggaagtg ttcagcgaaa atcaggacat
    17461 cgtggtcgcc acgaccggac gtctgctgca atacataaaa gaagagaact tcgattgccg
    17521 cgcggttgaa acgctgatcc tcgacgaagc agaccgtatg ctggatatgg gcttcgctca
    17581 ggatatcgaa catattgctg gcgaaacgcg ctggcgtaaa cagaccctgc tcttttcggc
    17641 aacgctggaa ggcgatgcga ttcaggactt tgccgagcgt ctgctggaag atccggtgga
    17701 ggtttctgcc aatccgtcca cccgtgagcg caaaaaaatt catcagtggt attaccgcgc
    17761 cgatgacctg gaacataaaa ccgcattgct ggtgcatctg ttaaaacagc cggaagcgac
    17821 ccgctcaatt gtgtttgtgc gtaagcgtga gcgtgtgcat gagctggcaa actggctgcg
    17881 cgaagcgggc atcaacaact gctatctcga aggtgagatg gtacagggca agcgtaacga
    17941 agcgatcaaa cgtttgaccg aaggtcgcgt aaacgtactg gttgcaaccg atgttgccgc
    18001 gcgcggtatc gacattcctg acgtcagcca cgtctttaac ttcgatatgc cgcgcagtgg
    18061 cgatacttat ttgcaccgta tcggacgtac cgcgcgcgcc ggtcgtaaag gcaccgcaat
    18121 ttcgctggta gaagcccatg accatctgct gctgggcaaa gttggtcgct atattgaaga
    18181 gccaattaaa gctcgcgtaa ttgatgagtt acgcccgaaa acgcgtgcac caagcgagaa
    18241 gcagaccggc aagccatcga agaaagtact ggcaaaacgc gccgagaaga aaaaagcaaa
    18301 agagaaagag aagccgcggg tgaaaaaacg ccatcgcgat accaaaaata ttggtaagcg
    18361 ccgtaaacca agcggaacgg gcgtgccacc gcaaacgaca gaagagtaat ctcaatgccg
    18421 ggtttaagcc cggcattatt tttatcccgg caaaaaacaa tcttcaacac actgaataaa
    18481 agtgtgcatc gccgggctaa ccgcttttcc ggcatggtga gcgcacattg ccgtaatggt
    18541 ctgcgagtgt tcgccaaaag gcaattcaat taattctccg ctctcaagct ctttttccac
    18601 cgcaaagcgg ggcaagtaac tgacaccgat attcgccgcg acacaacgtt tgatactttc
    18661 aatgcttaac agctcaatgg tgttttccac cgtgatccgc cgctggcgca gcgtgctctc
    18721 aaatatctgc cggaatacac attgcggttc gttgataata aagctacagg cgttatgtct
    18781 tcccggctca gtaaaatcga catctgcaat ttgcggtgaa gctaccagca ccagcgattg
    18841 ctcacccaat tctcgtcggt tcagggcatc atcattccct acgcgataaa aaaccccgac
    18901 atcggcctca tcattcaata gcgcatcacg gatcacgtaa cagttcagag actgcaacga
    18961 taaacgcact tttggggcgc gttgccgaaa acgctgcaac acctgcggca ttcggtaaga
    19021 gagcaacgtt tcgcccgaaa caacgcgtag ttccccgtcc ggatccgact cctttttggc
    19081 agcctcacga agggtatcca tcactctggt gagttcataa atgtgcggca gcagcttttt
    19141 accttcgcga gtgaggcaca tccgtcggcc aattttctca aataactgta ccgagaactc
    19201 ctgctcaagc tgctgaatat gaaaagtcac cgtcgattgt gtacagcaca atttttgcga
    19261 agctcgcaaa aaggaaccct cttccaccac ggttttaagc gtaataaaac ggcgcagatc
    19321 cataacccca aacctatcga aaatatcgaa tttagaatgt aaaaacattc atttttttaa
    19381 atattctgtg tcgggtactg tctaccaaaa cagaggagat aacaagtgac accgaccctt
    19441 ttaagtgctt tttggactta caccctgatt accgccatga cgccaggacc gaacaatatt
    19501 ctcgccctta gctcagccac aacgcacgga tttcatcaga gtacccgcgt gctggcaggg
    19561 atgagtctgg gatttttaat cgtcatgttg ctgtgtgcgg gcatttcgtt ttcactggct
    19621 gttatcgacc cggcagctgt gcatctgttg agttgggcgg gggcggcata tattgtctgg
    19681 ctggcgtgga aaatcgctac cagtccaaca aaggaagacg ggcttcagac aaaaccaatc
    19741 agtttttggg ccagctttgc tttgcagttt gtgaacgtca aaatcatttt gtacggtgtt
    19801 acggcactgt cgacatttgt tctgccgcaa acacaggcgt taagctggat agttggcgtc
    19861 agcgttttgc tggcaatgat tgggacgttt ggtaatgtgt gctgggcgct ggcggggcat
    19921 ctgtttcagc gattgtttcg ccagtatggt cgccagttaa atatcgtgct ggcgctgctg
    19981 ctgatctatt gcgcggtacg cattttctat taatgaaaaa aagcggaaga ggtcgccctc
    20041 ttccgcttag taacatgcta cttaagcctt acaggctttc agtaaaggta cgagcgataa
    20101 cgtcgcgctg ctgttccgga gtcagagagt taaagcgaac tgcataaccg gatacacgga
    20161 tggtcagctg cggatatttt tccggatgct taactgcatc ttccagagtt tcacgacgca
    20221 gaacgttaac gttcaggtgc tgaccacctt caacgcgaac ttctggtttc acttctactg
    20281 gaacttcacg gtactcaatg tcacccagtt tgcttactgc aaccacttcg tcttctgcat
    20341 aacctgcttt cgcaacgatg caacgcgctt cgcctttttc gctgtccagc agccagaaag
    20401 agttcagcag atcgtcgtta gcggctttag taatctggat acctgtaatc atgtgatgcc
    20461 tccccggcaa aattatttga tttgttcagc ctgtcgcggc caattggtaa aaccattgtt
    20521 gcttgagtgt atatatactc ctcaaacacc cttgaatctt tgatttaaat caataaaaac
    20581 cacacatcaa gtgtggtcgc aaatagattt tattgtttta catcaactta tgcgggtgtg
    20641 aaattttacc aatttacatt tttttgcact cgtttaagtc taaaaaatga gcatgatttt
    20701 gttctgtaga aagaagcagt taagctaggc ggattgaaga ttcgcaggag agcgagatgg
    20761 ctaacgaatt aacctggcat gacgtgctgg ctgaagagaa gcagcaaccc tattttctta
    20821 atacccttca gaccgtcgcc agcgagcggc agtccggcgt cactatctat ccaccacaaa
    20881 aagatgtctt taacgcgttc cgctttaccg agttgggtga cgttaaagtg gtgattctcg
    20941 gccaggatcc ttatcacgga ccgggacagg cgcatgggct ggcattttcc gttcgccccg
    21001 gaatcgccac tcctccgtca ctgctgaata tgtataaaga gctggaaaat actattccgg
    21061 gcttcacccg ccctaatcat ggttatcttg aaagctgggc gcgtcagggc gttctgctac
    21121 tcaatactgt gttgacggta cgcgcaggtc aggcgcattc ccacgccagc ctcggctggg
    21181 aaaccttcac cgataaagtg atcagcctga ttaaccagca tcgcgaaggc gtggtgtttt
    21241 tgttgtgggg atcgcatgcg caaaagaaag gggcgattat agataagcaa cgccatcatg
    21301 tactgaaagc accgcatccg tcgccgcttt cggctcatcg tggattcttt ggctgcaacc
    21361 attttgtgct ggcaaatcag tggctggaac aacatggcga gacgccgatt gactggatgc
    21421 cagtattacc ggcagagagt gagtaaattt gcgggaaaat gccggatggc agagttgcca
    21481 cccggctgat ttatcaggct ttattctgac gccaccattc gccaagaaga acgccggttg
    21541 cgacagagat attcagcccg gcaacgttgc cagtaccatc aatcttcacg cgcagatcgt
    21601 tcggatcgcg tgcggcatcc ggtaaccctt catattcctg acccagcacc agtaccattt
    21661 tcgctggcag actggttttg aacagcggtt taccctgctc gctggaagtt gtcactacgg
    21721 tgtaacccgc ctgacggaaa tcatccagca cgttaacaat gttgtcgcca gtaatcggct
    21781 gaacgtgctc tgcgccgcct tctgcggtac ggatagccgc ccccgactcc agcagtgccg
    21841 catcctgcac cacaacacct ttcacgccaa agtgcgcgca gctgcgcatc atgccaccca
    21901 ggttatgcgg gttagattcg ttttccagtg ccagaacaca atcctgcgcg cctgcctggc
    21961 ttacccactg ctgcacggtt gtgccattac gctttttgat caggaagcaa acgccaccgt
    22021 gatgttccgt gcctgacgct tttgtcaatt ccgcttcatc taccacatgg tacgctttgc
    22081 ggtttgctgc catccagcgc aaggcttctt taaaacgcgg cgttacactc tggataaacc
    22141 aggcgcgaac aatcgcttcc ggacggctct ggaacagtgc ctgacaggcg ttttcgccgt
    22201 agacgcgggt ttcttccgca cgctgacgac gcaacacttc cggatcaata aaacttttac
    22261 cactgatgcc accgtgatcg gccttttccg gcgtctcatc acccggcgcg cgggaaaccg
    22321 tgcgccacgg cgaatcttcc cacttgcggt cacggggctg actctgtttg tcatcgcggg
    22381 cggggcgacg gccaccgtca gcacgagatt ttcctggacg cccgccccct ttcccggtac
    22441 gcgggttgtg ggtacgttta tcagaatcat catcactgcg gacatacatc actttgacct
    22501 tgccgctttt acctttcatt tcatcgttca tgcttttctc caccagcgct gcgcgaagcg
    22561 cgcagattac ccgaagtccg cgcggttcgc catgatttcg taccaaagcc tgcgactatc
    22621 atacctattg aataaaacag attgttgtct ggaacaatgt ccccgataat atgtaacata
    22681 ttagaaacat accggcgttg ttgccgataa gtctccttac tcatcccgag gttagttatg
    22741 aataccgttt gtacccattg tcaggccatc aatcgcattc ccgacgatcg gatcgaagat
    22801 gcggcaaaat gcggacgctg cggtcacgac ttgtttgacg gagaggtgat taatgcgacc
    22861 ggtgaaacgc tcgacaaatt gctgaaagat gatctaccag tggtgatcga cttctgggca
    22921 ccgtggtgcg gcccctgccg taatttcgca ccaatttttg aagatgtcgc gcaagagcgt
    22981 agcggtaaag tgcgctttgt gaaagtgaat accgaagctg aacgtgaatt gagcagtcgc
    23041 tttggaattc gtagtatacc gacgatcatg attttcaaaa acggtcaggt tgtcgacatg
    23101 cttaatggcg cagtaccgaa agcgccgttc gatagctggc tgaacgaatc tctttaatct
    23161 taccggggcg catcttgtgc cccgttttct cctctgcgac aatggcgttt tttcgacgct
    23221 ctcttatgac cgaaaacgct gttctccagt tacgcgccga gcgtattgcg cgcgcaacac
    23281 gtccttttct tgcccgcggt aatcgcgttc gtcgctgcca acgctgtctt ttgccagaga
    23341 aattatgtct ctgttcgaca attaccccag cacaagcaaa aagtcgcttc tgtttgctga
    23401 tgttcgacac cgagccaatg aagcccagta ataccgggcg tctgattgct gatattttgc
    23461 ctgataccgt tgcgtttcaa tggtcgcgta ccgaaccctc gcaggatttg ctggatctgg
    23521 tacaaaaccc ggattatcag ccaatggtgg tctttcccgc ctcgtatgct gatgagcaac
    23581 gggaagtgat cttcacacca cctgccggta agccaccgct gtttatcatg ctcgatggta
    23641 cctggccgga agctcgcaag atgtttcgta aaagtccgta tctggataat cttcccgtca
    23701 tttccgtcga tctttcccga ctttctgcct atcgcctgcg tgaagcccag gctgaaggcc
    23761 aatattgtac tgccgaggta gccatcgcac tgttagatat ggccggcgat accggggcgg
    23821 cagcagggtt aggcgagcac ttcacccgct ttaaaacgcg ctatctggca ggaaaaacgc
    23881 aacatctggg tagcatcaca gcagaacagt tagaaagcgt ttaaaatcat tcggtcactt
    23941 ctgcgggaga ccggtatgag tcagcgagga ctggaagcac tactgcgacc aaaatcgata
    24001 gcggtaattg gcgcgtcgat gaaacccaat cgcgcaggtt acctgatgat gcgtaacctg
    24061 ctggcgggag gctttaacgg accggtactc ccggtgacgc cagcctggaa agcggtgttg
    24121 ggtgtgttgg cctggccgga tattgccagc ttgcccttta cacccgacct tgcggtttta
    24181 tgtaccaatg ccagccgtaa tcttgctctt ctggaagagc tcggcgagaa aggctgtaaa
    24241 acctgcatta ttctttccgc cccggcatcg caacacgaag atctccgcgc ctgcgccctg
    24301 cgccataaca tgcgcctgct tggaccaaac agtctgggtt tactggctcc ctggcaaggt
    24361 ctgaatgcca gcttttcgcc tgtgccgatt aaacgcggca agctggcgtt tatttcacag
    24421 tcggctgccg tctccaacac catcctcgac tgggcgcaac agcgtgagat gggcttttcc
    24481 tactttattg cgctcggcga cagcctagat atcgacgttg atgaattgct tgactatctg
    24541 gcacgcgaca gtaaaaccag cgccatcctg ctctatctcg aacagttaag cgacgcgcga
    24601 cgctttgttt cggcggcccg tagtgcctcg cgtaataaac cgattctggt gattaaaagc
    24661 ggacgtagcc cggcagcaca gcgactgctc aacacgacgg caggaatgga tccggcgtgg
    24721 gatgcggcga ttcagcgtgc cggtttgttg cgggtgcagg atacccacga gctgttttcg
    24781 gcggtggaaa ccctcagcca tatgcgcccg ctgcgtggcg accggttgat gattatcagc
    24841 aacggtgctg cgcctgccgc gctggcgctg gatgccttat ggtcacgcaa tggcaagctg
    24901 gcaacgctaa gcgaagaaac ctgccagaaa ctgcgcgatg cactgctagg acatgtggca
    24961 atctctaacc cgctcgatct gcgcgatgac gccagcagcg agcattatgt caaaacgctg
    25021 gacattctgc tccacagcca ggattttgat gcgctgatgg ttattcattc gcccagcgcc
    25081 gctgctcccg caacagaaat cgcgcaagta ttaattgaag cggtaaagca tcatccccgc
    25141 agcaagtatg tttctctgct gacgaactgg tgcggcgagc actcctcgca agaggcacga
    25201 cgtttattca gcgaagccgg gctgccgacc taccgtaccc cggaaggaac catcactgct
    25261 tttatgcata tggtggagta ccggcgtaat cagaagcaac tacgcgaaac gccggcgttg
    25321 cccagcaatc tgacttccaa taccgcaaaa gcgcatcttc tgttgcaaca ggcgattgcc
    25381 gaaggggcta cgtcgctcga tacccatgaa gttcagccca tcctgcaatc gtatgggatg
    25441 aacacgctcc ctacctggat tgccagcgat agcaccgaag cggtgcatat tgccgaacag
    25501 attggttatc cggtggcgct gaaattgcgt tcgccggata ttccacataa atcggaagtt
    25561 cagggcgtca tgctgtacct gcgtacagcc aatgaagtcc agcaagcggc aaacgctatt
    25621 ttcgatcgcg taaaaatgac ctggccgcag gcgcgggtcc acggcctgtt ggtgcaaagt
    25681 atggctaacc gtgctggcgc tcaggagttg cgggttgtgg ttgagcacga tccggttttt
    25741 gggccgttga tcatgctggg tgaaggcggt gtggagtggc gtcctgaaga tcaagccgtc
    25801 gtcgcactgc cgccgctgaa tatgaacctg gcccgctatc tggttattca ggggatcaaa
    25861 agtaaaaaga ttcgtgcgcg cagtgcgcta cgcccattgg atgttgcagg cttgagccag
    25921 cttctggtgc aggtttccaa cttgattgtc gattgcccgg aaattcagcg tctggatatt
    25981 catcctttgc tggcttctgg cagtgaattt accgcgctgg atgtcactct ggatatcgcg
    26041 ccgtttgaag gcgataacga gagtcggctg gcagtgcgcc cttatccgca tcagctggaa
    26101 gaatgggtag aattgaaaaa cggtgaacgc tgcttgttcc gcccgatttt gccagaagat
    26161 gagccacaac ttcagcaatt catttcgcgg gtcaccaaag aagatcttta ttaccgctac
    26221 tttagcgaga tcaacgaatt tacccatgaa gatttagcca acatgacgca gatcgactac
    26281 gatcgggaaa tggcgtttgt agcggtacga cgaattgatc aaacggaaga gatcctcggc
    26341 gtcacgcgtg cgatctccga ccctgataac atcgatgccg aatttgccgt gctggttcgc
    26401 tcggatctca aagggttagg cttgggtcga cgcttaatgg aaaagttgat tacctatacg
    26461 cgagatcacg gactacaacg tctgaatggt attacgatgc caaacaatcg tggcatggtg
    26521 gcgctggccc gcaagctcgg gtttaacgtt gatatccagc tcgaagaggg gatcgttggg
    26581 cttacgctaa atcttgccca acgcgaggaa tcatgagtaa ggtactggaa atgttgacca
    26641 cttaatcggg actggtgtta ttattgcacg cttatgtcgt ctgcattgca cagaggaccc
    26701 ttcaatgaac agagaagaaa tgcactgtga tgttgtcaaa atttaagcgt aataaacatc
    26761 aacaacacct tgcccaacta cccaagattt ctcaatcagt tgatgatgtc gatttctttt
    26821 acgctcccgc cgacttccgg gagacgctgc tggaaaaaat agccagcgcg aagcagcgca
    26881 tttgcattgt cgccctgtat ctcgaacagg atgacggtgg caaaggcatt ctgaacgcgt
    26941 tgtatgaagc taaaaggcag cgtccggaac tggatgtgcg ggtgctgctc gactggcatc
    27001 gtgcacaacg tggacgcatt ggcgctgcgg catctaacac taacgctgac tggtactgtc
    27061 gcatggcgca ggaaaatccg ggcgtagatg ttccggttta tggcgttcca atcaataccc
    27121 gtgaagccct tggtgttctg cactttaaag gctttatcat cgacgatagc gtactttata
    27181 gcggtgccag cctgaacgat gtttacctgc atcagcacga taaatatcgc tacgaccgtt
    27241 atcatcttat ccgtaaccgt aagatgtcag acattatgtt tgaatgggtt acacagaata
    27301 ttatgaatgg ccgcggcgtt aatcgtctgg atgatgttaa tcggccaaaa agcccggaaa
    27361 tcaagaacga tattcgtctg ttccgccagg agctgcgtga tgccgcttat catttccagg
    27421 gcgatgccga caacgatcag ctttccgtaa cgccgctagt ggggctgggg aaatcgagtc
    27481 tgttgaacaa gaccattttc catcttatgc cttgtgccga gcagaaacta accatctgta
    27541 cgccatactt caacctgcca gcaatccttg tgcgcaatat tatccagttg ctgcgcgaag
    27601 ggaaaaaggt cgaaattatt gttggtgata aaaccgcgaa cgacttctac attccggaag
    27661 atgaaccttt caagataatt ggcgcattgc cttatctcta tgagatcaat ctgcgtcgtt
    27721 tcctgagccg tttgcagtat tacgtcaata ctgaccagct agtggttcgg ttatggaaag
    27781 atgacgacaa cacctatcac ctaaaaggga tgtgggttga tgataagtgg atgttgatca
    27841 ccggtaataa cctgaacccg cgcgcctggc gtctggatct ggaaaacgcc attttgatcc
    27901 acgatccaca actggagctt gcgccgcagc gagagaaaga actggagctg atccgcgagc
    27961 ataccaccat cgttaagcac tatcgcgatc tgcaaagtat tgccgattat ccggtgaagg
    28021 ttcgtaaact catccgccgt ttgcgccgta tccgcatcga ccgattaatt agccgcatcc
    28081 tgtaatcaca accccgtcct gtacggggtt tgttttttgg aggccacgtt ttgcgtattc
    28141 tttttgtctg ttcgctattg ttactttctg gatgcagcca tatggctaac gatagctgga
    28201 gcgggcagga taaagctcaa cactttatcg cctcggcgat gctttccgcc gccggaaatg
    28261 aatattcaca gcatcagggg atgagccggg atcgcagtgc catgtttggt ttgatgttct
    28321 ctgtcagttt gggggcgtca aaagagtttt gggatagccg ccccgaaggg agcggctgga
    28381 gctggaagga tttggcctgg gatgtcgccg gtgcaagcac cggctatacc gtctggcaac
    28441 tgacccgtca ctaaagacgc atccccttcc ctttgcgatg tagcatcaaa gaaaccagaa
    28501 acgccaccac ggccatcaag gtcacatacc agaagaaggc tgtttccatt cctattgatt
    28561 tcagcgacaa cgctacgtac tccgccgaac caccaaatat agcattagcg accgcatatg
    28621 acagaccaac gcctaatgcg cgaacctgtg ccgggaacat ctcagccttc agtattccac
    28681 tgattgatgt ataaaaactc actatcagca gggcacacat caccagacca aaagcggcat
    28741 aaggcgagga aacgttttgc aatgctgaga gaataggaac ggtaaaaatg gctgccagcg
    28801 aaccgaaaca taacattgag gtacggcgac caatcttatc cgacagcgcg ccaatgagtg
    28861 gttgaataag catgaataca aacaatgcgg cagtcataat gccactcgcc acgttggcat
    28921 gcattcccgc agtatttacc agatacttct gcatataagt ggtgaaggta tagaaacaaa
    28981 gggagcccgc agcggtaaaa ccgagaacca tgatgaatgc acggcgattg cgccataatc
    29041 ctttcagaga tccagcttct tttaaagcgc gcgtttcttg ttgcgaagtt tcatctaact
    29101 gacgacgtaa ccacaacgcc acaacagcta acacagctcc taacgcgaaa ggaatacgcc
    29161 atccccactc tctgagtgca gagtcttcca tggtgtgttg taaaaccacg acaaccagta
    29221 gggctagcag ttgtccgccg atcaacgtca catactgaaa tgatgcgtaa aaacctttgc
    29281 gcccttcaac ggcaacttca ctcatatagg tggcgctggt gccatattct ccgccaacgg
    29341 ataatccctg aaataaacgc gcgagaagca ataatgccgg agcccacgta ccgattgttt
    29401 cataaccagg caaacaggcg attaccagtg agccgaaaca catcatacac accgatagca
    29461 gcatcgattt tttgcgacca tgtttatcgg ctatgcggcc aaatagccaa ccgcctattg
    29521 ggcgcatcag gaatcccgca gcaaaaacac ctgctgtttg tagtagttga gtcgtcgtgt
    29581 tcccggaagg gaagaagatg tgggcaaagt agagtgaaca gaacgagtag acatagaaat
    29641 cgaaccactc gaccagatta cctgaagagg cccccacaat cgcccaaatg cggcgacgag
    29701 tatcactact tgtcagtttg ctgtctgccg ttacagtact ttcagccatg ccattatgtc
    29761 tcctgccgta atccgatgct tttgtcggtc gcttttgttt atttcttttg gcaaagaaaa
    29821 tgttatacat ttgttacgta tcattatgca accttaacca tgaatttagt tagcaggaaa
    29881 atggttattg aggagcttaa ggataaattt ctggtaagga ggacacgtat ggaagtaggc
    29941 aagttgggga aaccgtatcc gttgctgaat ctggcatatg tgggagtata agacgcgcag
    30001 cgtcgcatca ggcatttttt tcaccaacgc aaaaaggcca tcctgacgga tggccttt
//
LOCUS       DBFIFS010000037        29897 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.037, whole genome shotgun sequence.
ACCESSION   DBFIFS010000037 DBFIFS010000000
VERSION     DBFIFS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.037"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            137..1720
                     /gene="yegH"
                     /locus_tag="N7A07_001529"
     CDS             137..1720
                     /gene="yegH"
                     /locus_tag="N7A07_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU2476218.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1795..2133
                     /locus_tag="N7A07_001530"
     CDS             1795..2133
                     /locus_tag="N7A07_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2476219.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2123..2413
                     /locus_tag="N7A07_001531"
     CDS             2123..2413
                     /locus_tag="N7A07_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2476220.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2466..4319)
                     /gene="asmA"
                     /locus_tag="N7A07_001532"
     CDS             complement(2466..4319)
                     /gene="asmA"
                     /locus_tag="N7A07_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU2476221.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAAQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4341..4922)
                     /gene="dcd"
                     /locus_tag="N7A07_001533"
     CDS             complement(4341..4922)
                     /gene="dcd"
                     /locus_tag="N7A07_001533"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU2476222.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5014..5655)
                     /gene="udk"
                     /locus_tag="N7A07_001534"
     CDS             complement(5014..5655)
                     /gene="udk"
                     /locus_tag="N7A07_001534"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU2476223.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5973..9291
                     /gene="dgcE"
                     /locus_tag="N7A07_001535"
                     /pseudo
     CDS             5973..9291
                     /gene="dgcE"
                     /locus_tag="N7A07_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9392..10240)
                     /gene="alkA"
                     /locus_tag="N7A07_001536"
     CDS             complement(9392..10240)
                     /gene="alkA"
                     /locus_tag="N7A07_001536"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU2476224.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10374..11726
                     /gene="yegD"
                     /locus_tag="N7A07_001537"
     CDS             10374..11726
                     /gene="yegD"
                     /locus_tag="N7A07_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2476225.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11739..13679)
                     /gene="yegI"
                     /locus_tag="N7A07_001538"
     CDS             complement(11739..13679)
                     /gene="yegI"
                     /locus_tag="N7A07_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU2476226.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13676..14437)
                     /gene="pphC"
                     /locus_tag="N7A07_001539"
     CDS             complement(13676..14437)
                     /gene="pphC"
                     /locus_tag="N7A07_001539"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU2476227.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14434..15093)
                     /gene="yegL"
                     /locus_tag="N7A07_001540"
     CDS             complement(14434..15093)
                     /gene="yegL"
                     /locus_tag="N7A07_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU2476228.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15314..15373)
                     /gene="ibsA"
                     /locus_tag="N7A07_001541"
     CDS             complement(15314..15373)
                     /gene="ibsA"
                     /locus_tag="N7A07_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU2476229.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15645..15701)
                     /locus_tag="N7A07_001542"
     CDS             complement(15645..15701)
                     /locus_tag="N7A07_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2476230.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15973..16029)
                     /locus_tag="N7A07_001543"
     CDS             complement(15973..16029)
                     /locus_tag="N7A07_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2476231.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16309..17556
                     /gene="mdtA"
                     /locus_tag="N7A07_001544"
     CDS             16309..17556
                     /gene="mdtA"
                     /locus_tag="N7A07_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU2476232.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17556..20678
                     /gene="mdtB"
                     /locus_tag="N7A07_001545"
     CDS             17556..20678
                     /gene="mdtB"
                     /locus_tag="N7A07_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU2476233.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAITLVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20679..23756
                     /gene="mdtC"
                     /locus_tag="N7A07_001546"
     CDS             20679..23756
                     /gene="mdtC"
                     /locus_tag="N7A07_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU2476234.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23757..25172
                     /gene="mdtD"
                     /locus_tag="N7A07_001547"
     CDS             23757..25172
                     /gene="mdtD"
                     /locus_tag="N7A07_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476235.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25169..26572
                     /gene="baeS"
                     /locus_tag="N7A07_001548"
     CDS             25169..26572
                     /gene="baeS"
                     /locus_tag="N7A07_001548"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU2476236.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26569..27291
                     /gene="baeR"
                     /locus_tag="N7A07_001549"
     CDS             26569..27291
                     /gene="baeR"
                     /locus_tag="N7A07_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU2476237.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27482..27814
                     /gene="yegP"
                     /locus_tag="N7A07_001550"
     CDS             27482..27814
                     /gene="yegP"
                     /locus_tag="N7A07_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU2476238.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27962..29323
                     /gene="yegQ"
                     /locus_tag="N7A07_001551"
     CDS             27962..29323
                     /gene="yegQ"
                     /locus_tag="N7A07_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU2476239.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29653..>29897)
                     /gene="yegR"
                     /locus_tag="N7A07_001552"
     CDS             complement(29653..>29897)
                     /gene="yegR"
                     /locus_tag="N7A07_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU2476240.1"
                     /translation="SIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVKND
                     AAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6905 a         7977 c         7897 g         7118 t
ORIGIN      
        1 taattcaggg ataatgtgag gttattaccc tcaaataata tgagataaat agtgctgcaa
       61 cattgcattt ttgccccgat ttatccatga tcgaattgtg acatttgtca tacaacgaat
      121 aggttttgta cttactatgg aatggattgc cgatccgtct atctgggccg ggttaatcac
      181 gctgattgtg atcgaactgg tcctcggcat tgataacctg gtctttattg ccatcctcgc
      241 cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt accgggctgt tgctggcgat
      301 gttaatgcgc ctgttactgc tggcgtcaat ctcctggctg gtcaccctga ctcaaccgct
      361 gttcagcttc cgctcgttta cctttagcgc ccgcgactta atcatgctgt ttggtggttt
      421 cttcctgttg ttcaaagcca cgatggagct gaacgaacgg ctggaaggga aagacagcaa
      481 taatcccaca caacgcaaag gtgcgaagtt ctggggggtg gtgacacaaa ttgtggtact
      541 ggacgccatc ttctcactcg actcagtgat taccgccgtc gggatggtcg accatttact
      601 ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg ttgatggcca gcaagccgtt
      661 aacgcaattt gttaacagtc acccgaccat cgttattctc tgcttaagct tcctgttgat
      721 gattggcttt agcctggtgg cagaaggttt cggcttcgtc attccgaaag gctacctgta
      781 cgctgccatt ggtttctcgg tgatgatcga ggcgctcaat cagttggcta tctttaaccg
      841 gcgacgtttt ctttccgcta accagacact gcgccagcgg actaccgaag cggtaatgcg
      901 cctgcttagc gggcaaaaag aggatgcgga actggacgcc gaaaccgcgt ccatgctggt
      961 ggatcatggt aaccagcaga tctttaatcc gcaggaacgg cggatgattg agcgggtact
     1021 taatcttaac cagcgtaccg tcagcagcat tatgacgtcg cgccacgata ttgagcatat
     1081 cgatctcaac gcgccggaag acgagatccg ccagttgctg gagcgaaatc agcatacgcg
     1141 gctggtggtt actgatggcg atgacgcgga agatttgctc ggtgttgttc acgttatcga
     1201 cctgttacaa caatcactgc gcggcgaacc gctcaacctg cgggtgttaa ttcgccagcc
     1261 gctggtgttc ccggaaacct tgccgttgtt acctgccctg gagcagttcc gtaatgcccg
     1321 cacacacttt gcttttgtgg tggatgagtt tggctcggtg gaagggattg tgacattaag
     1381 tgacgtcact gaaaccattg ccggtaactt accgaacgaa gtggaagaga tcgacgcccg
     1441 ccatgatatt cagaagaatg cggacggttc ctggacggcg aatggtcata tgccgctgga
     1501 agatctggtg caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc acaccattgc
     1561 cgggctattg atggaatatt tgcagcgtat tccaaaacct ggcgaagaag ttcaggtggg
     1621 ggattatttg cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg tgcagattat
     1681 accgctgcgt aaggatggcg agatggagta cgaggtgtga taaaacatca aatgcgcagg
     1741 ttcaaactgc gcatttgatt tatacaaaat ttagtataat ctaaggatgg agtaatgaga
     1801 aaaaagctgg cttttcttga taccagtctt gacgatttac gcgcatttcc agaatcgtct
     1861 cgacaggaaa ttggctatca actcgacagg atccaacaag ggttaaaccc atacgactgg
     1921 aaaccttttt caactatcgg ccctggtgtg cgagagattc gtactcgcga tgctgatggg
     1981 atctatcgcg tcatgtatgt cgcaaaattt gaagaagcgg tttacgtatt gcattgtttt
     2041 caaaagaaaa cacagactac cagtcaatcg gatattgatt tagccaaacg acgctataaa
     2101 gaacttgttc aggagcggaa aaatgaaaat tgaaaccttt gatagtgtgt gggatgcggt
     2161 gagtgacacc cctgaacaag cggaaaatat gcggatccgt gccgagcttg taacgatcat
     2221 taataactgg attgaacaac aagggtttag ccaggctcag gcagcttctg ctttaggggt
     2281 cactcaacct cgcatctctg aacttgccag aggtaaaatc cagatcttta gtatcgataa
     2341 attgatcacc atgatggctc atgcaggact acatattcaa cgtattgaag ttcaatatcc
     2401 acatgctgct tagtgctata aatacagatg gcgacaaatt gatgccgcca tctatataac
     2461 ttgtcttaca tcttctccag caacttcttc acatctttgc cattgcggga atctttattc
     2521 cgctcggccc agtcattcag gcgacgtttc gcttcgtcct gtagatgttt gcgcagtaac
     2581 tgatccactt gcagactgta attgagttgc tgccagttgc cataaacccg cagcggtact
     2641 ggcgtttctt tgaggaaatc aatcagtttg ctttccccgt tccagccacc cacgacccga
     2701 atatcaaact gggtgtcgca ggtttgagct gccagattca acatgccttc ccctgtaagc
     2761 gccagcacag gcgattgacc ttgcatgtcg tttaacgtca cgacgccatc cttcaacgtc
     2821 aaatcggtgg taaagcggtc aagacgcgtt acgttatcga agttttcagc ggccttcaca
     2881 tcaccaccat tacgttctac cgcttgctga atcatctgct ggaagttcat cccttccatg
     2941 cgcgtatcgg ccatttcgac atgtgcctgt ccttgccagt tgtggcggaa tgcgtcggca
     3001 tctatgtcag caccggagaa atcaccagcc aatgacattt ttccggtcaa cgaaatcgga
     3061 tagttaaacg ccttcagaat ggtgccaatc tcaacgtttt ccagccgtgg ctggaagttt
     3121 atccgcggat ttattgatgt cgcgtccagc gtgcccggca gtgaaacctg tccaccgtta
     3181 agcttgccct gtagttgagt aatttccagc aaacccgact tgttggtcat ttgcgtggca
     3241 acatctgtaa aattcattcc gcgccagcgc acgttactgg cctgcaacaa aatatcagcc
     3301 gtaaagcctt gcagtccctg ataggccggt tcatcaatac gcgaagaaat aaccgggcgc
     3361 ggcaacgttg attggctctg cccctgctgc gcggcaccgt tttcaccatt cgcggtttca
     3421 ttaagcggga tgaggttgtc aagattcagt tgcgggaatt gcagcctcag ctgccattcc
     3481 ggtttctctg tcagcgtgac ctgtgcttgc ccgctcagcg tactatcatt ggcggtcaaa
     3541 ctaatttggt taaatgaaag gcgtttacgt gactcctgcc actgggcctg gaaactcccc
     3601 tgcccctgaa taccttgttt tggtaaatcg gcaccctgca actgccagtt aatttgttca
     3661 atagccgccg ttaaatcatg cggataatca gaagcatcta ccgtaccgtt aagggatatc
     3721 gtgagatcgc gctgatcgcg attaacccgc ccggagaact caaatgagcc acgatgttgg
     3781 ggatcttgtt ccatttgcag gcggatattg cggattgtca cctgctcgtc atcttcatgc
     3841 tggaacacca gcacgctgtc cgccacctta agactggata tatcaaacga ccatccgcga
     3901 tcgtctgaca gatccggcaa ggtattgtcg cgcggtgcaa ccggagcgtc ttcactgcgc
     3961 accgcttccg tctgcggcgt cagttggatc actgcccctt ttagcatcac ctgcttaacg
     4021 ctcagttgat gactcagtag tggtaaaagc gccacgtcca ggcgcatgtt gtcggcgcga
     4081 accagtggct ggctcgcgcc ctgggcggtg agagacattc gcccggagag gatactaagc
     4141 tgcggccaga cgtgccaacg cagtggcccg tcgagctgca attgataacc gctacgtgca
     4201 gcaacttgct tgaccatata gtcgcggaaa tcattcggat tcaccagcaa cactaacgca
     4261 gataacccgg ccaccagcac gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg
     4321 gtatcctcaa tgggcctgaa ttagtcttta tcgattcggc tggctaccgc gccctgctgg
     4381 ttgcgatact tcgcatcttc acggcggttg taaggtcgcg ccgccgggcc ggaaagcggc
     4441 tcaaagctca gcgcaccaat taacatgccc ggacgcagcg ccagcggcag cttaccggag
     4501 ttgtagaact ccagcacaat gcaaccagac cagcccggat cgatacggtg cgcggtgacg
     4561 tgcaccatca gccccagacg cgccagtgag gaacgcccgt ccagccagcc caccagatcg
     4621 gctggcagcg tcaccgactc cagcgtcacc gccagcgcca gctctcctgg gtgaagatag
     4681 aacgcctcgc tttcatcgag aacgatctca tcgctcatca cgcggtcaag cgcggcgctc
     4741 acttcatctt tgggaccgct cagatcgata aacgctgccg tgtgaccacg gaaggtacga
     4801 aatttattgc ccaggcgtac atccaccgtc gcgccgttaa tacgctccac tggcggacgt
     4861 gggttgatcg acaaacggcc ttcatcaagc caggcttcaa tatctcggtc acacagacgc
     4921 atggcatttt ctcctttcgc gcatcactcc cttaacgcca attacgtcaa gggcatacca
     4981 ggttatcact gaacggtaca caatttatca agcttattca aagaactgac ttattttcgc
     5041 tttcaatata tcgatcgcga tgcggttttt cccgccgcgc ggcacgataa tgtccgcata
     5101 ttgtttagaa ggctcaatga attgcaggaa catcgggcgc acggtttttt gatattgcgc
     5161 catcacggaa tccattgaac gcccacgctc gttaacgtca cgcttgatgc ggcgcatcag
     5221 gcagatatcc agcggggtat caacgaaaat ggagaagtta agttcgtcac gcaaacgtgc
     5281 atccgtcagc aacaaaatgc cttcgagaat gatgaccttc ttcggctcaa ccgtcaccgt
     5341 ttctttcata cgcgtatgtt caacatagct gtaaaccggc aggtcaattg ccgagccgcg
     5401 tttcaacgct tgtaaatgct caagcagcag gctgtgatcc atcgcgctgg gatggtcgta
     5461 gttggtctta acgcgttctt ccatcgacag atggctttga tctttgtaat agcagtcttc
     5521 gggaattacg ccgatgtgtt catcaccgac ttgctcacgt aattcacgat aaagggtact
     5581 ggcaataaga ctcttgccgg aagccgatgc gccagcgata ccgataatga cgcactgatg
     5641 agactgatca gtcatatatt tagcgacctg attaacctgg atgttaggaa gggggcgacg
     5701 aagcgccaaa cgcggcaatt atagggattt catccgcctg ataccagtcg aatagcgttg
     5761 ccgcgcgctc agagttaatt gttgacgaag aattcccggt ggcaaattac gttgatcagt
     5821 tttatacaag gtaaaaattg ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt
     5881 tatgagcaat tcgcatataa aatgtaaaac ttttgtacta gcataaacac agaaacgaat
     5941 actggcgacc tggccttgcg gataaagcgg taatgagcaa acaatcacag catgtattaa
     6001 ttgccctgcc ccacccgctg cttcacctgg tcagtttagg tttagtctcg tttatcttca
     6061 cccttttctc gcttgagctt tcgcagtttg gcacccaact cgccccactg tggttcccga
     6121 cgtccatcat gatggtggcg ttttatcgcc atgccgggcg catgtggcca gggattgcgc
     6181 tgagctgctc gctgggaaat atcgccgcat ccatcctgct tttttccacc agctcgctga
     6241 acatgacctg cacgaccatc aatattgttg aagccgtggt cggggcagtg ctgctacgta
     6301 aattgctgcc gtggtataac cccttgcaaa atctggctga ctggctgcgt ctggcactcg
     6361 gcagcgccat tgttccacct ctgttggggg gtgttctggt tgtcctgctg acgcccggag
     6421 acgatcctca tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca
     6481 ctggtgccgc tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccacgg
     6541 ttgctttttg agtcgctgct cacgttagcc atcacactga cgttaagctg gctttcgatg
     6601 ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgc
     6661 ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt gtcgctgatg
     6721 atggccgcgg atccctccct gcttgctacg ccgcgtacgt acctgatgag ccatatgccg
     6781 tggctaccgt ttttgctgat cctgctgccc gccaacatca tgacgatggt gatgtatgcc
     6841 tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gttttcggaa cgccatggaa
     6901 tattccgcca tcggcatggc attagtgggc accgagggac aatggctgca atccaacaaa
     6961 gcactctgcc agtttctcgg ttacagtcag gaagagctgc gcggactcac ctttcagcaa
     7021 ctgacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct gataagcggt
     7081 gaaataaaca cctattcaat ggaaaaacgt tactacaacc gcaatggcga tgttgtctgg
     7141 gcgttgcttg ccgtctctct ggtgcgccac acggatggca cgccgctcta ttttatcgct
     7201 cagattgaag acattaacga gctaaaacgc accgaacagg tgaatcagca actgatggaa
     7261 cgcatcacgc tggctaacga agcgggcggg attggcatct gggagtggga actgaagccg
     7321 aatattttta gctgggataa gcggatgttc gagctgtatg aaattcctcc gcatatcaaa
     7381 ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccaaca cgccgaaaaa
     7441 gtgattcgtg attcgttgca atcacgctcg ccctttaaac tggaatttcg cattgccgtg
     7501 aaagatggca tccgccatat ccgcgccctc gccaaccggg tactgaataa agaaggcgaa
     7561 gtcgaacgcc tgctcggcat taatatggat atgactgagg ttaaacagct taacgaggca
     7621 ttgtttcagg aaaaagagcg cctgcacatt acgctggact ccatcggcga agccgtggtc
     7681 tgtattgata tggcgatgaa aattaccttt atgaatccag tggcggagaa gatgagcggc
     7741 tggacgcagg aagaagcgtt aggtgttccg ctcctgacgg tgttgcatat tacttttggc
     7801 gacaacggac cattaatgga gaacatttac agtgccgaca cctcacgttc cgcgatcgaa
     7861 caagatgtgg tgttgcactg tcggagcggc ggcagctacg acgtgcatta cagtattacg
     7921 ccgttaagta ctctggacgg cagcaatata ggctcggtac tggtgattca ggacgttacc
     7981 gaatcacgca aaatgctgcg ccagctgagc tacagcgcct cccatgatgc actgacgcat
     8041 ctcgccaacc gcgccagttt tgagaaacaa ctgcgtatcc tgctgcaaac ggtaaacagt
     8101 acgcatcagc gacatgcact ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac
     8161 agcgccgggc atgcggcggg cgacgctttg ctgcgcgaac tggcgtcgtt gatgctgagt
     8221 atgctgcgct ccagtgacgt gctggcgcgg ctcggcggtg atgaatttgg tctgctgctg
     8281 ccagactgca atgttgaaag cgctcgtttt atcgctacac gtattatcag tgctgtgaat
     8341 gactatcact ttatatggga aggccgggta catcgggtag gtgccagcgc cgggattacc
     8401 ttgatcgatg acaacaatca tcaggcggca gaagtgatgt cgcaggctga tatcgcctgt
     8461 tatgcctcca aaaatggtgg ccggggccgg gtgacggttt acgaaccgca gcaagctgcc
     8521 gcacatagcg aacgggcggc gatgtcgctt gatgaacagt ggcggatgat taaagagaat
     8581 cagttgatga tgctcgccca cggtgtcgct tcgccacgga tcccggaagc gcgtaatttg
     8641 tggctgattt cacttaagct ctggagttgc gaaggcgaga ttattgatga acaaacattt
     8701 cgtcgtagct tcagcgatcc ggcgcttagc catgctcttg accgccgggt attccacgaa
     8761 tttctccagc aggccgcaaa agcggttgcc agtaaaggca taagcatcgc cctccccctt
     8821 tccgttgccg gtttgagtag cgccacgctg gtgaatgatc tgcttgagca gctggaaaat
     8881 agccctctac caccacggtt attacatctg attattccgg cagaagcgat tttcgatcac
     8941 gcagaaagcg tgcaaaaact gcggctgggg ggatgccgga tcgtattcag tcaggtgggc
     9001 cgcgatctgc aaatcttcaa ctcgctgaaa gcgaatatgg cagattacct gctacttgat
     9061 ggtgagttat gcgccaacgt gcagggtaat ttgatggatg agatgctgat tacgattatt
     9121 caggggcacg ctcagcgact cgggatgaaa accatcgccg ggccagtcgt tttaccctta
     9181 gtgatggata cgctttctgg catcggcgtc gatctgattt atggtgaggt gattgccgat
     9241 gcccaaccgc tggatttgct gatgaatagt agttatttcg cgattaactg agggagcgtt
     9301 tgcccattgc ctgatgcgac gctaacgcgt cttatcaggc ctacaaatcg ctcattccgc
     9361 aaggcgctcg caccgcatcc ggcgaccaac gtcatgcttc gtctggttgc cagccttccg
     9421 tataccagat atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc
     9481 ggatttgcgc cggtgtcatt cccggaaatc gctgtttaat cagataatca tccggcagga
     9541 aaacatcttt cgcctgccag ccacgcaaag caaaataatt cgccgtccag cgcccgatac
     9601 ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc gcccggtatt gtcattggta
     9661 aggtgccctc cagcgccgca tttgccagat gaatcagcgc ctctgcccgt ttcaacggca
     9721 tacctaacgc ttttaatgcc tgcgggtctg ctgctgccag ccgctgaggc gtggggaagc
     9781 agatatactc cggaaaatca tccagccgtt cgccataaag ctgtaccact ttggcagtca
     9841 attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcacg ccttgctcaa
     9901 aagcatcaac actgccgggt aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg
     9961 caccgttaac aatctgcggg ttacattgca gatcaagcag gcggctcatt ttcgccagac
    10021 actcagcggc aacgggttct aaatctgcac ttaaatttat gtgcagagta tggcgggcta
    10081 tatccggaat agcagtcacc acgccgcgat attcgcccac cgccagacta cgggcataat
    10141 aatcgtcagc gaccgtttcc acaccactca ctgcacgggc ggcgagaaat cccaacatcc
    10201 acgaccagtc atacggcggc tgccagttca gggtatacat cgcatctcct tattcatccg
    10261 ctttcagcat aaacgttatt cagacgctgc gctttgcttt catattccgg ttgtcgcgac
    10321 ggcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattat ttcgtgttta
    10381 ttggttttga ttacggtaca gcaaactgtt cagtagcggt catgcgtgac ggtaaaccgc
    10441 acttgctaaa aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc
    10501 gtgaagcggt aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa
    10561 cgcaggcact gttgcgtcgg gcgattcgtt ataatcgcga agaagatatc gatgttacgg
    10621 cgaaaagcgt gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag
    10681 tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg
    10741 tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcaag
    10801 cgcagctgcc agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc
    10861 tgggcggcga tgaagcaaac gcccaggcgc aagggattct ggaacgcgcg gcgaagcgtg
    10921 ccggattcag ggacgtggta ttccagtacg agccggtagc ggctgggctg gattacgaag
    10981 ccaccttaca ggaagaaaaa cgggtactgg tggtggatat cggcggtggt acgactgact
    11041 gttcattgct gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc
    11101 tgggtcacag tggttgccgt attggcggta acgatctgga tatcgcactg gcgtttaaaa
    11161 acctgatgcc actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgattc
    11221 tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta
    11281 gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg
    11341 ccctgctaca gaaagtctgg cgtcagcgtt taagctatcg cctggtgcgt agcgcagaag
    11401 agtgcaaaat tgcgctttca agcgtagcgg aaacccgcgc ctcactgccg tttatcagcg
    11461 atgaactggc tacgctgatt agccagcaag ggctggaaag cgccctcagc cagccactgg
    11521 cgcggattca ggaacaggtg caactggcgc tggataacgc ccaggaaaaa ccggacgtta
    11581 tctacctgac cggcggaagc gcccgttctc cgctgattaa aaaagcgctg gcagaacagt
    11641 tgccgggcat tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac
    11701 gctgggcgga agtggtgttt cgttaattga aaaatggctc agcagcggct taaatctgcc
    11761 tgcgcctgag ccagtttttc tgccgcctga cgtaacggtt ccatcaacgg catggtccgt
    11821 gctggagcat gaaggcggaa tcgctgcaac tctgtcgcgc cgacagtcaa cgccgattcc
    11881 agccgatgtc gtttggcggc cattttcgcc attaccgctt gtctttctgc aggcgttacc
    11941 gcttcgttcg gcctgaacac aaaacggcgt tcacagctcg ctttccagtc gataaccgcc
    12001 tgggtcagat gatcaccaaa ccctttaact tgcttaaccc cacgacgggt aacatccgcg
    12061 gctgtttcaa taccaaaaga ccgtaacgcc gctttacgcg cagggccaac gccgggaata
    12121 gaggcagcat caataaaaaa tccctccaga aacttctgct tctgccgttc ccttgcagtg
    12181 tcatgaagtg ctgccagagc gcgtttctcc tcttccggta acccgagaat ttcgtccttc
    12241 attttttcga gcatcgcccg tttggcgata aaaccttcca gcccgcccaa ctgttggatc
    12301 tggctgacta aatggtcata gtccattttc gcgcgattaa atgcctcacg gcgttgctgg
    12361 atttctgctt tataaacttt gcttgtcaga ctgccaataa tccagatagc cgccagcaca
    12421 ggaaccaaaa taatataacg cggttctgcc tgaaggccgc aaagcaacag cgataacgct
    12481 gacagtgcga tctcaatcag aatgatgtat tcgcgccgta acaggcctaa aggaagcggc
    12541 ctgccagtcg gttggaaatg atcgggtaat ggcaattgca atgctggcgg tgctactgac
    12601 gccatcacca tcgcccagac tttcgccagc acaaaatcac cactggtggt aatgacctct
    12661 tcgccgagat caataaaata gataacgcct tgattatcca gcgcacacca cgggcagtca
    12721 gtcaaatgac cggggtaaac atgcattgcc gaaacggtac atttctttaa ctgttggcgt
    12781 agtaaatcca gtgccgctac ccacgctttt gccgtcggac gcgcggttgc cacgccactt
    12841 tccgtgaatg cctgctgaaa catggcttca acatcgcccg gtaacatcga cagcggaatc
    12901 gatcgtggcg gcggttttaa accacgtcgc tggttgtctg acgcgtaggc ataacggaaa
    12961 tgggcaatat ccgtctccag cgcattaccc gcatcagaga taagcggcac accagaataa
    13021 ggatgccgcc caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa attatcgtga
    13081 ttcgcggtac gttcaaaacc gacaaatgat ggcaaggttt gtagctctgg cggcgtaaaa
    13141 tgcgacacgc cgacttcgca taaatgcagt gtgccattgg cgttaatctg aaaggagtca
    13201 ctatcgatca acaccacttt gctgtcgcga cctaccataa agctgttctg gtttacgtcc
    13261 cccacgacgt gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca
    13321 tagagtagaa aatcccacgc acaatgagga taactctggc gacgatgtgc cgggctatag
    13381 atcatatgaa tcggttcttt accagaaact tttggcatca taaaaccgat aacttttccg
    13441 cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat aattcaacaa ctgcgcgtca
    13501 gctgtcgcag ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa
    13561 atcttggcga cgctatcgac aaactcattg atatcataaa ccgcgccttc gccacctttg
    13621 cccagttcac ggcctggcgt aacgcactca ccagtagcag tatataaagt gggtttcatg
    13681 gtaaccacag cgccagtgtc aacgttttat catcatcggt acgctcgttt accgctgggc
    13741 tcgacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc gttgctgccg
    13801 ccagtccatt gaaaaacggg gtaaagaaag gcacatgcgg agaattatcc agcatattta
    13861 acgccagtcg ctgaatacca tccgtaaatg ctgcaacttt atgcgcacgc tcagtgctgg
    13921 tgaaagtttc cagtctggaa acgacatctt catcggtaat gaaatgcgtc atattggcat
    13981 attcacccac catcggcgta agcggtaatt gcagaccgtg accaaaatcc accaccacgc
    14041 cgccatcgcc aatctgcata atcaaggtgc cgttagccga tgaaatcaat cccagaaacg
    14101 tgcaggcaaa atcgcgaact gctaattctt tagcttccgc ctcggcaaaa agtcgctgac
    14161 gaacggtcag cacaatatcc gtggcgagaa tatcattgag tccaaattca ccgtgctgca
    14221 ctttttgcga cacataagcc atcgcttcat tgatggcaag catcgcgcct tcgccaccct
    14281 gcgagacact gcctgcgcca tcagcaagga acatcaccag taatggctgc tggtcattta
    14341 gccaggcaac ctgcatctgg caggcatcct gacaaggtaa atcagcgcta atgtgcgacg
    14401 ttccaacagc ggatgcataa accagacgcc agctcacact gacgtccagc cttttggcgc
    14461 ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg aactggataa
    14521 ccagctaaac agttcacgga attgtaatcc ctgcagtggc aaaggctgac gaacgctgat
    14581 ttgtgccaga gtcttaatat ccgccccctg aacaccgatg gaaaagaagg caaacttctt
    14641 atcttcttcc ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg ttggtgcacc
    14701 atcggtgatc aggaaaatcc acgggcgata atacgaaata ccatttgcac gatactcacg
    14761 tttccgctcc tctaccatat ccagggcttt ggtaatggcg gcacccattg gtgtatcccc
    14821 ctgggcaaac aggatgggcg ggaaaaaatt agcggcgctg gtaaagggtt gttccacatg
    14881 caccgggccg aacgtcacaa taccaagttc cactcttttt aaggccagcg ggtcggcaag
    14941 cagctcatcg cgaaaggtaa ccaatccggc gttaagttca ttaatgggtc taccgttcat
    15001 cgaaccagaa acatccagca gcaaaatgca ggggcaacgt ggttccgggt tactggcaaa
    15061 atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag agaattattg
    15121 aggtccggta tcataccgat tgcaataatg aaataagagc aaacgaagaa actttcagaa
    15181 ttaggcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag agccgtcgat
    15241 tgacactaag ggcggagtga cataatttca ggagtgaggg ttagggaaag gtttccccct
    15301 ccccctggtg ttcttagtaa gcctggaagc taatcactaa gagtatcacc agtatgatga
    15361 cgtgcttcat cataaccctt ttcttattta aggccccttc ctcgggaggg gctttcccgt
    15421 ttcagcatcc cgctgaaatc gtcggcttac ctcctttcgc catgcatgca gtctatcgct
    15481 gacttgcgga taaaatagtt tccagtgtga ttattgcgta acgtctcgca aagaatattt
    15541 gctggcagga tcgcagactg caaagcctgc agattgacaa tcttatcgtg aaggcatact
    15601 ttcaggagtg agggtagagc ggggtttccc ccgccctggt agtcttagta agccgggaag
    15661 cttatgacta agagcaccac gatgatgatc cacttcatca tgaccctttc cttatttaag
    15721 gccccttcct cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct
    15781 cctttcgccc tgaattcagt ctatcgctga cttgcggata aaatagtttc ctctgtgatt
    15841 attgcgtaac gtctcgcaaa gaatatttgc tggcaggatc gcagactgca aagcctgcgg
    15901 attgacattc tgaacgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc
    15961 gccctggtag tcttagtatg ctgggtagct aatcactaag agcaccacga tgatgagtat
    16021 cttcatcatg accctttcct tatttatggc cccttcctcg ggagggggct ttcccgtttc
    16081 agcgtcccgc tgaaatcgtc ggcttacctc ctttcgccat gcaagcagtc tatcgctaac
    16141 gcgtagataa aatagtttcc tgtgttatta ctggatgcgt gctcgcaaat gtgcccgcca
    16201 ttcagacgat tcccgatagt gtttcataat tcctccattt ttctccctta ttggctggct
    16261 acactagtat cattccgcga aacgtttcag gaagagaaac tcttaacgat gaaaggcagt
    16321 tataaatccc gttgggtaat cgtaatcgtg gtagttatcg ccgccatcgc cgcattctgg
    16381 ttctggcaag gccgcaatga ctcccagagt gcagccccag gggcgacgaa acaagcgcag
    16441 caatcgccag cgggtggtcg ccgtggtatg cgttccggcc cattagcccc ggttcaggcg
    16501 gcgaccgccg tagaacaggc agttccgcgt tacctcaccg ggcttggcac cattaccgct
    16561 gctaatactg ttacggtgcg tagccgcgtg gacggtcaac tgatggcgtt acatttccag
    16621 gaaggccagc aggtcaaagc tggcgattta ctggcagaaa ttgaccccag ccagttcaaa
    16681 gttgcattag cacaagccca gggccaactg gcaaaagata aagccacgct taccaacgcc
    16741 cgccgtgatc tggcgcgtta tcagcaactg gcaaaaacca atctcgtctc ccgccaggag
    16801 ctggatgccc aacaggcgct ggtcagtgaa accgaaggca ccattaaggc tgatgaagca
    16861 agcgtcgcca gcgcgcagct gcaactcgac tggagccgta ttaccgcacc agtcgatggt
    16921 cgcgttggtc tcaagcaggt tgatgttggt aaccaaatct ccagtggtga taccaccggg
    16981 atcgtggtga tcacccagac gcatcctatc gatttgctct ttaccctgcc ggaaagcgat
    17041 atcgctaccg tcgtgcaggc gcaaaaagcc ggaaaaccgc tggtggtaga agcctgggat
    17101 cgcaccaact cgaagaaatt aagtgaaggc acgctgttaa gtctcgataa ccaaatcgat
    17161 gccactaccg gtacgattaa agtgaaagca cgctttaata atcaggatga tgcgctgttt
    17221 cccaatcagt ttgttaacgc gcgcatgtta gtcgacaccg aacaaaacgc cgtagtgatc
    17281 ccaacagccg ccctgcaaat gggcaatgaa ggccattttg tctgggtgct gaatagcgaa
    17341 aacaaggtca gcaaacatct ggtgacgccg ggcattcagg acagtcagaa agtggtgatc
    17401 cgcgcaggta tttctgcggg cgatcgcgtg gtgacagacg gcattgatcg cctgaccgaa
    17461 ggggcgaaag tggaagtggt ggaagcccag agcgccacca ctccggaaga gaaagccacc
    17521 agccgcgaat acgcgaaaaa aggagctcgc tcctgatgca agtgttaccc ccgagcagca
    17581 caggcggccc gtcgcgcctg tttattatgc gtcctgtggc caccacgctg ctgatggtgg
    17641 cgatcttact cgccgggatt atcggttatc gcgccctgcc cgtttcggcg ctgccggaag
    17701 tggactatcc gaccattcag gtggtcacgc tctacccagg tgcgagcccg gatgtcatga
    17761 cctctgccgt taccgcgccg ctcgaacgcc agttcgggca gatgtctggc ctgaaacaga
    17821 tgtcgtcgca aagttccggc ggtgcgtcag ttatcacttt acagttccag ctaacattac
    17881 cgctggatgt cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc aacttgttgc
    17941 cgagcgatct gcctaacccg ccggtttaca gcaaagtgaa cccggcagat ccgccgatca
    18001 tgacgctcgc cgtcacctca acagccatgc cgatgacgca agtggaagat atggtggaaa
    18061 cccgcgtcgc gcagaaaatc tcgcagattt ccggcgtcgg cctggtgacg ctttccggcg
    18121 gccagcgtcc ggctgttcgc gtcaaactta acgctcaggc gattgccgcc ctcggcctga
    18181 ccagcgaaac cgtgcgcacc gccattaccg gcgctaacgt taactcggct aaaggtagcc
    18241 ttgacggccc ttcccgtgcg gttacgcttt ccgctaacga ccagatgcaa tccgccgaag
    18301 agtatcgcca gctaatcatc gcctaccaga acggcgcgcc gattcgtctg ggcgatgtcg
    18361 caactgtaga gcaaggtgca gaaaacagct ggctcggcgc atgggcgaat aaagaacagg
    18421 ccattgtgat gaatgttcag cgccagcccg gtgctaacat tatctccacc gccgacagca
    18481 tccggcagat gctgccacag ctcaccgaga gtctgccgaa atcggtgaag gtgacggtac
    18541 tttctgaccg taccaccaat atccgcgcat ccgtcgatga tactcagttt gaattgatga
    18601 tggctatcac gctggtagtc atgattatct acctgttttt gcgcaatatt ccggcgacca
    18661 tcattcccgg cgttgcagtg ccgctgtcgt taatcggcac cttcgcggtc atggtgtttc
    18721 tcgatttttc aatcaataac ctgacgctga tggcgttaac tatcgccacc gggttcgtgg
    18781 tcgatgacgc catcgtggtg atcgaaaata tttcccgcta tatcgaaaaa ggcgaaaaac
    18841 cgttggcggc ggcgctcaaa ggcgcaggtg aaatcggctt taccattatc tcgctgacct
    18901 tctcgctgat tgcggtgttg atcccactgc tgtttatggg cgatatcgtc gggcgactgt
    18961 tccgcgaatt tgctattacc ctggcggtag cgattttaat ctcagccgtg gtgtcgctaa
    19021 ccctgacacc gatgatgtgc gcacggatgc tcagccagga gtcgttgcgt aaacagaacc
    19081 gcttctcccg tgcctcggaa aaaatgttcg acaggataat cgccgcctat ggtcgtggac
    19141 tggcgaaagt gctgaatcat ccgtggctga ccttaagcgt ggcgctcagc acgctgctgc
    19201 ttagcgttct attgtgggtg ttcattccga aaggtttctt cccggtacag gataacggca
    19261 ttattcaggg cactttgcag gcaccgcaat ccagctcctt tgccaatatg gcccagcggc
    19321 aacgccaggt cgcggacgtg attttgcagg atccggcagt gcaaagtctg acctcatttg
    19381 ttggcgttga tggcactaac ccgtcgctga acagtgcgcg tttgcagatc aacctcaaac
    19441 cgttggatga acgtgatgat cgggtgcaaa aagtcatcgc ccgtctgcaa acggcggtgg
    19501 ataaagtgcc gggcgtcgat ctcttcctgc aaccaacgca ggacctgacc atcgatactc
    19561 aggtcagccg cacccagtac cagtttacct tgcaggccac gtcactggat gcgctcagta
    19621 cctgggtgcc acagttgatg gaaaaactcc agcagctgcc acagatttct gatgtctcca
    19681 gcgactggca ggacaaaggg ctggtggcgt atgtcaatgt tgatcgcgac agcgccagcc
    19741 gtctggggat cagcatggcg gatgtcgata acgccctgta caacgcgttt ggtcagcgac
    19801 tgatttccac tatttatact caggctaacc agtatcgtgt agtgctggag cacaataccg
    19861 aaaatacccc aggcctcgcg gcgctggata ccattcgcct gaccagcagc gacggcggcg
    19921 tggtgccgct aagctcaatt gccaaaattg agcagcgttt tgcgccgctt tccatcaacc
    19981 atctggatca gttcccggta acgaccatct cctttaacgt gccggataac tattcgctgg
    20041 gcgatgcggt gcaggcaatt atggacaccg agaagacgct gaatctgccg gtggatatca
    20101 ccacgcaatt ccagggcagc accctcgcct tccagtcggc gctgggcagc actgtctggc
    20161 tgattgtcgc ggcggtggtg gcgatgtata tcgtgctcgg cattctgtac gagagcttta
    20221 ttcacccgat caccattctc tcgacgctac ccacggcagg tgttggcgcc ctgctggcgt
    20281 taatgattgc cggtagcgaa ctggatgtga ttgcgattat cggcattatt ttgctgatcg
    20341 gtatcgtgaa gaagaacgcc atcatgatga tcgacttcgc gctggctgct gagcgcgagc
    20401 aaggcatgtc gccgcgcgat gcaatctacc aggcttgtct gttgcgtttt cgtccgatcc
    20461 tgatgaccac cctggcggct ctgcttggcg cgctgccgct gatgttgagt accggggtcg
    20521 gcgcggaact acgtcgtccg ttaggtatcg gcatggtcgg cggcctgatt gtcagccagg
    20581 tgctgacgct gttcaccacg ccggtgattt atctgctgtt tgaccgcctg gcattgtgga
    20641 ccaaaagtcg ctttgctcgc catgaagagg aggcgtaagt gaagtttttt gccctcttca
    20701 tttaccgccc ggtggcgacg attttactgt cggtcgccat taccctgtgc ggcatactgg
    20761 gtttccgtat gctgccggtc gccccgctgc cgcaggtcga ttttccggtg attatggtca
    20821 gcgcctcgct gcccggtgcg tcgccggaga caatggcgtc ttccgttgcc acgccgctgg
    20881 agcgctcact tgggcgcatt gccggggtca gtgaaatgac ctccagcagt tcgctcggca
    20941 gcacgcgtat tattttgcag tttgattttg accgggatat caacggtgcg gcgcgtgatg
    21001 tgcaggcggc gatcaacgcc gcacaaagtc tgctgcccag tgggatgccc agccgcccga
    21061 cttatcgcaa agcgaacccg tcggatgcgc caattatgat cctcacgcta acctccgata
    21121 cttattcgca gggtgaactg tacgatttcg cctcgacgca gctggctccg acgatttcgc
    21181 aaatcgacgg tgtcggtgat gtcgatgtcg gcggcagctc gctgcccgcc gtgcgcgtcg
    21241 ggctgaaccc gcaggcgctg tttaatcagg gcgtgtcgct ggacgacgta cgcactgcca
    21301 tcagcaatgc caacgtgcgt aaaccgcagg gggcgctgga agatggcact caccgctggc
    21361 agatccagac caacgatgaa ctgaaaaccg ccgctgaata tcagccgctg attattcact
    21421 acaacaacgg cggcgcggtt cgtctgggcg atgtggcgac ggtgaccgac tcagtgcagg
    21481 atgtgcgcaa cgccgggatg accaacgcca aaccggctat tttactgatg atccgcaaat
    21541 tgccggaagc caatattatc cagacggtag acagcatccg ggcaaaatta ccggagttgc
    21601 aggaaaccat tccggcggcg attgatctgc aaattgccca ggatcgctcc cccaccattc
    21661 gcgcctcgct ggaagaagtc gagcaaacgc tgattatctc ggtggcgctg gtgattctgg
    21721 tggtcttttt attcctgcgc tcgggtcgcg ccactattat tcccgccgtt gcggtgccgg
    21781 tttcgctgat tggtacgttt gcggcgatgt acctgtgcgg attcagtctc aataaccttt
    21841 cgttaatggc gctcaccatc gctactggtt tcgtggtgga tgacgccatc gtggtgctgg
    21901 aaaacattgc acgtcatctg gaagcgggaa tgaaaccgtt gcaagccgca ctgcaaggta
    21961 ctcgcgaagt cggttttacg gtgctgtcga tgagtctgtc gctggtggcg gtgttcctgc
    22021 cgctgctgtt gatgggcgga ttgccgggcc gactgttacg cgagtttgcc gtgacgcttt
    22081 ctgtcgccat tggtatttcg ttgctggttt ctctgacatt aacgccaatg atgtgtggct
    22141 ggatgctgaa agccagcaag ccgcgcgagc aaaagcgact gcgtggtttt ggtcgcatgt
    22201 tggtagccct gcaacaaggc tatggtaagt cactgaaatg ggtgctcaat catacccgtc
    22261 tggtgggcat ggtgctgctt ggcaccattg ctttgaatat ctggctatat atctcgattc
    22321 cgaaaacctt cttcccggag caggacactg gcgtgttgat gggcgggata caggcggatc
    22381 agagtatttc gtttcaggcg atgcgcggta agttgcagga tttcatgaaa attatccgtg
    22441 acgatccggc agtggataat gtcaccggct ttaccggcgg ttcgcgagtg aacagcggga
    22501 tgatgtttat caccctcaag ccacgcgacg aacgcagcga aacagcgcag caaattattg
    22561 accgtctacg cgtgaagctg gcgaaagaac cgggggcgaa tctgttcctg atggcggtac
    22621 aggatattcg cgttggcgga cgtcagtcga acgccagcta ccagtacacg ttgttatccg
    22681 acgacctggc ggcactgcgc gaatgggagc cgaaaatccg caaaaaactg gcgacattgc
    22741 cggaactggc ggacgtgaac tccgatcagc aggataacgg cgcggagatg aatctggttt
    22801 acgatcgcga caccatggca cggctgggaa tcgacgtaca ggccgccaac agcctgttaa
    22861 ataacgcctt cggtcagcgg caaatctcga ccatttacca gccgatgaac cagtataaag
    22921 tggtgatgga agtggatccg cgctataccc aggacatcag cgcgctggaa aaaatgttcg
    22981 taatcaataa cgaaggaaaa gcgatcccgc tgtcgtattt cgctaaatgg caaccggcga
    23041 atgccccact atcggtgaat catcagggat tatcggcagc ctcgaccatc tcgttcaacc
    23101 tgccgaccgg aaaatcgctc tcggacgcca gtgcggcgat cgatcgcgca atgacccagc
    23161 ttggtgtgcc ttcgacggtg cgcggcagtt ttgccggcac ggcgcaggtg ttccaggaga
    23221 cgatgaactc acaggtgatc ctgattatcg ccgccatcgc cacggtgtat atcgtgctgg
    23281 gtatccttta cgagagttac gtacatccgc tgacgattct ctccaccctg ccctcggcgg
    23341 gcgttggagc gctgctggcg ctggagctgt tcaatgcccc gttcagccta atcgccctga
    23401 tagggatcat gctattaatc ggcattgtga agaaaaacgc cattatgatg gtcgattttg
    23461 cgcttgaagc ccaacggcac ggtaacctga cgccgcagga agctattttc caggcctgtc
    23521 tgctgcgttt tcgcccgatt atgatgacta ccctggcggc gctgtttggc gcgctgccgc
    23581 tggtgttgtc gggcggtgac ggctcggagc tgcggcaacc cttggggatc accattgtcg
    23641 gcggtctggt gatgagtcag ctcctgacgc tgtataccac gccggtggta tatctctttt
    23701 tcgaccgtct gcggctgcgt ttttcgcgta aatctaaaca aacggtaacc gagtaaatga
    23761 cagatcttcc cgacagcacc cgctggcaat tgtggattgt ggctttcggc ttctttatgc
    23821 agtcgctgga caccaccatc gtaaacaccg cccttccctc aatggcgcaa agcctcgggg
    23881 aaagtccgtt gcatatgcac atggtcattg tctcttatgt gctgaccgtg gcggtgatgc
    23941 tgcccgccag cggctggctg gcggacaaag tcggcgtgcg caatattttc tttaccgcta
    24001 tcgtgctgtt tactctcggt tcactgtttt gcgcgctttc cggcacgctg aacgaactgt
    24061 tgctggcacg cgcgttacag ggcgttggcg gtgcgatgat ggtgccggtc ggcaggttga
    24121 cggtgatgaa aatcgtaccg cgcgagcaat atatggcggc gatgaccttt gtcacgttac
    24181 ccggtcaggt cggtccgctg ctcggtccgg cgctcggcgg tctgctggtg gagtacgcgt
    24241 cgtggcactg gatctttttg atcaacattc cggtggggat tatcggtgcg atcgccacat
    24301 tgatgttaat gccgaactac accatgcaga cgcggcgctt tgatctttcc ggctttttat
    24361 tgctggcggt tggcatggcg gtattaaccc tggcgctgga cggcagtaaa ggtacaggtt
    24421 tatcgccgct ggcgattact ggcctggtcg cagttggtgt ggtggcactg gtgctttatc
    24481 tgctgcacgc cagaaataac aaccgcgctc ttttcagtct gaaactgttc cgcactcgta
    24541 ccttttcgct gggcctggcg gggagctttg ccggacgtat cggtagcggc atgttgccct
    24601 ttatgacgcc ggttttcctg caaattggcc ttggcttctc accgtttcat gccggactga
    24661 tgatgatccc gatggtgctt ggcagcatgg ggatgaagcg gattgttgta caggtggtga
    24721 atcgctttgg ttatcgtcgg gtactggtag cgaccacgct gggtctgtcg ctggtcaccc
    24781 tgttgtttat gactaccgct ctgttgggct ggtactacgt tttgccgttc gtcctgtttt
    24841 tacaagggat ggtcaactcg acccgtttct cctccatgaa caccctgacg ctgaaagatc
    24901 tcccggacaa tctggcgagc agcggcaaca gtctgctgtc gatgattatg caattgtcga
    24961 tgagtatcgg cgtcaccatc gccgggctgt tgctgggact ttttggttcg cagcatgtca
    25021 gcgtcgacag cagcaccaca caaaccgtct ttatgtacac ctggcttagc atggcgttta
    25081 tcatcgccct tccggcgttc atctttgcca gagtgccgaa cgatacgcat caaaatgtag
    25141 ctatttcgcg gcgaaaaagg agcgcgcaat gaagttctgg cgacctggta ttaccggcaa
    25201 actgtttctg gcgattttcg ccacctgcat tgtcttgctg atcagtatgc actgggcggt
    25261 gcgtatcagt tttgagcgtg gctttattga ttacatcaag catggtaatg aacagcgatt
    25321 acaactgtta agtgatgcgc ttggcgagca gtatgcgcag catggcaact ggcgcttcct
    25381 gcgcaacaat gatcgctttg tctttcagat cctgcgttca tttgaacacg ataattcgga
    25441 agataaaccc ggcccgggta tgccaccaca cggctggcgt actcagttct gggtggttga
    25501 tcaaaacaac aaggtgctgg ttggtccgcg agcgccgatt ccacctgacg gtacacggcg
    25561 acccattctg gtcaacggtg cggaagtggg cgcggtgatt gcctcccctg ttgagcggct
    25621 gacccgcaat actgatatca atttcgataa acaacagcgg caaaccagct ggctgattgt
    25681 cgccctggca acgttactcg cggcactcgc tacttttctg ctggcgcgcg gtttgctggc
    25741 accggtaaaa cgacttgtcg atggcacgca caaactggcg gcgggcgatt tcactacccg
    25801 cgtaacgccc accagtgaag atgaactggg caaactggcg caagacttca accagctcgc
    25861 cagcacgctg gagaaaaacc agcaaatgcg gcgcgatttt atggccgata tttctcacga
    25921 actgcgtacg ccattagcgg tgctgcgcgg tgaactggaa gccattcagg atggcgtgcg
    25981 caaattcacg ccggagacgg tggcgtcttt acaggcggag gtcggtacac tgaccaaact
    26041 ggttgacgat cttcatcagt tgtcgatgtc tgatgaaggc gctctcgcct atcaaaaagc
    26101 accggtagat ttgatcccac tgctggaagt ggcaggcggc gcatttcgcg aacgatttgc
    26161 cagccgtggc ctgaaactgc aattttccct gccagacagt atcaccgtat ttggcgatcg
    26221 cgaccgttta atgcagttat tcaataactt actggaaaac agcctgcgct acactgacag
    26281 cggcggcagc ctgaaaatct ctgccgagca gcacgacaaa acggtgcgcc tgacctttgc
    26341 cgacagtgcg ccaggtgtca gtgacgatca gctacaaaaa ttgtttgaac gtttttatcg
    26401 caccgaaggt tcccgcaacc gcgccagcgg cggttccggg ctggggctgg cgatttgcct
    26461 gaacattgtt gaagcacata atggccgcat tattgccgcc cattcgcctt ttggcggggt
    26521 aagcattaca gtagagttac cgctggaacg ggatttacag agagaagtat gaccgagtta
    26581 ccaatcgacg aaaacacacc gcgtattttg atcgtggaag atgaaccgaa gctgggacag
    26641 ttgctcattg attatctgcg tgctgcgagc tatgcgccga cgcttatcag ccacggcgat
    26701 caggtactgt cgtacgtgcg ccagacgccg ccggatctga tcctgttgga tctgatgctc
    26761 cctggcaccg atggcctgac gctgtgccgg gaaattcgtc gtttttctga cattcctatc
    26821 gtgatggtga cagcaaaaat cgaagagatc gaccgcctgc tagggctgga gattggcgca
    26881 gatgactaca tttgcaagcc gtacagccca cgggaagtgg tagcgcgtgt caaaaccatt
    26941 ttgcgccgtt gcaaaccgca gcgtgagttg cagcaacagg atgctgaaag cccgttgatt
    27001 atcgacgaag gtcgttttca ggcttcatgg cgcggtaaaa tgcttgacct gacgcctgcg
    27061 gaatttcgtc tgctgaaaac gctctctcat gaaccaggaa aagtgttctc ccgcgagcaa
    27121 ttgctcaatc atctttatga cgactaccgc gtagtaaccg accgtaccat cgacagccac
    27181 attaaaaacc tgcgccgcaa gctggaatct ctcgacgccg aacagtcatt tatccgcgcc
    27241 gtttacggcg tcggttaccg ctgggaagcc gacgcctgcc gcatcgttta gttttagcga
    27301 cattactttg ttagccggag atggcattca gaccagatcc ggcaacatta tcccacgcat
    27361 ggtcagcaaa ctgacattat ctctccgccc tggcaggcat cagaaaatcg ctcatacttt
    27421 aatcggtaaa cagcaccttt agatgctgtt ttcgatacgc aatttcaatc aaggagtcat
    27481 tatggctggt tggtttgaac tcagtaagag cagtgatagt cagttccggt ttgtgctaaa
    27541 agcgggcaat ggtgagacga tcctcaccag cgagctgtat acctcaaaag cctctgcgga
    27601 aaagggcatc gcgtcggtgc gtagcaacag cccgcaagaa gaacgctatg agaaaaaaac
    27661 ggcaagcaac ggcaaattct atttcaatct gaaagccgcc aatcatcaaa ttatcggctc
    27721 cagccagatg tatgccaccg cgcaatctcg tgaaaccgga attgcctccg ttaaagccaa
    27781 tggcacaagc cagacggtga aagacaatac gtaattacga tgccgggcgt gggttgttgg
    27841 cgtccggcgc tttaccagat cccccctaca gcgctacaat gcccgccctt aaagtggggg
    27901 cactccccta accgcttcat caggtgaagc ggatctgacc tgtcatcaga acgagagaat
    27961 tatgtttaaa ccggaactcc tttccccggc gggaacgctg aaaaatatgc gttacgcttt
    28021 cgcttatggc gcagatgctg tttatgcggg ccagccgcgt tactccctgc gtgtgcgcaa
    28081 caacgaattc aaccacgaaa accttcagct cggcatcaat gaagcccacg cgctggggaa
    28141 aaagttttat gtcgtggtca acattgcacc gcacaacgcc aagctgaaaa cctttatccg
    28201 tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg attatgtccg atccagggct
    28261 gattatgctg gtgcgtgagc acttccctga aatgccgatc catctctcgg tacaggctaa
    28321 cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct
    28381 ctctcgcgag ctgtcgctgg aagagattga agagatccgc aatcaggtgc cggatatgga
    28441 gatcgagatc ttcgttcacg gcgcactgtg catggcctac tccggtcgct gcctgctctc
    28501 tggctatatc aacaagcgcg atccgaacca gggtacctgc accaacgcct gccgctggga
    28561 gtacaacgtc caggaaggga aagaagatga cgttggcaac atcgtacaca agtacgagcc
    28621 gattccggtg caaaatgttg agccgacgct gggtatcggc gcgccaaccg acaaagtgtt
    28681 tatgatcgaa gaagcccagc gtccgggcga gtatatgacc gcgtttgaag atgagcacgg
    28741 cacttacatc atgaactcga aagatctgcg cgccatcgcc catgtagaac gcctgaccaa
    28801 aatgggcgtg cattcgctga aaatcgaagg ccgtactaaa tctttctact attgcgcacg
    28861 caccgcacag gtttatcgta aagctatcga tgatgccgct gcgggcaaac cgttcgatac
    28921 cagcctgctg gaaactctgg aaggtctggc gcatcgtggc tataccgaag gtttcctgcg
    28981 tcgtcatact cacgacgatt atcagaacta cgaatacggt tattcagttt ctgaccgcca
    29041 gcagtttgtt ggtgagttta ccggtgagcg caagggggac ctcgcggcgg tagcggtgaa
    29101 aaataaattc tccgttggcg acagccttga gctgatgacg ccgcaaggca acattaactt
    29161 tacccttgag cacatggaaa acgccaaagg cgaagctatg ccggtagcac caggcgatgg
    29221 ttatactgtg tggctcccgg tgccgcagga tcttgagcta aattacgcgc tgctgatgcg
    29281 taatttctcc ggggaaacta cgcgtaaccc ccacggtaag tgattaattt cgattatttt
    29341 tcccggatgg aaaattctta gaaaccgatc acatacagct gcatttatta aggttatcat
    29401 ccgtttcgct gaaaaacata acccataaaa tgctagctgt accaggaacc acctccttag
    29461 cctgtgtaat ctcccttaca cgggcttatt ttttatgcat aagccctatc cctgataacc
    29521 gtcttccatt gaccacatcg atagaatctc ccttcatagc acgatgcctt tcacgtaacg
    29581 gcatcgtgct cgcacaggtt ccggctaagc acaaccagaa cgcgcatgtt tgacgcttac
    29641 caaaaaatat tctcactctc cacatttgaa tgtcagacga gcgacgccat gtaatcctgc
    29701 accttctgtc ttcaggtcaa ctatctgcat ttttttgccc tgagtaacac agaaatgggc
    29761 tgcatcattt tttactatat tttctgcacc agatattctg cccctggcta aagaagcttc
    29821 ggcttcggtg tagtattggt tatcgagttt acgctgaata ttacttttat atgcaagacc
    29881 aaatttaccg atacttg
//
LOCUS       DBFIFS010000038        28571 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.038, whole genome shotgun sequence.
ACCESSION   DBFIFS010000038 DBFIFS010000000
VERSION     DBFIFS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.038"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..827
                     /locus_tag="N7A07_001553"
     CDS             <1..827
                     /locus_tag="N7A07_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2476241.1"
                     /translation="DLHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDM
                     NAYHRGMVTLACIPTAVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDF
                     GINMNNVTNSSIDFTPLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNR
                     LLIEQQLADKPWKLDWFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLV
                     EPVIRRTLGIIRRKDAVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(868..1863)
                     /gene="pgl"
                     /locus_tag="N7A07_001554"
     CDS             complement(868..1863)
                     /gene="pgl"
                     /locus_tag="N7A07_001554"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU2476242.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2018..2836
                     /gene="ybhA"
                     /locus_tag="N7A07_001555"
     CDS             2018..2836
                     /gene="ybhA"
                     /locus_tag="N7A07_001555"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU2476243.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2837..3895)
                     /gene="modC"
                     /locus_tag="N7A07_001556"
     CDS             complement(2837..3895)
                     /gene="modC"
                     /locus_tag="N7A07_001556"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU2476244.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(3898..4587)
                     /gene="modB"
                     /locus_tag="N7A07_001557"
     CDS             complement(3898..4587)
                     /gene="modB"
                     /locus_tag="N7A07_001557"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU2476245.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4587..5360)
                     /gene="modA"
                     /locus_tag="N7A07_001558"
     CDS             complement(4587..5360)
                     /gene="modA"
                     /locus_tag="N7A07_001558"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2476246.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5527..5676)
                     /gene="acrZ"
                     /locus_tag="N7A07_001559"
     CDS             complement(5527..5676)
                     /gene="acrZ"
                     /locus_tag="N7A07_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU2476247.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5805..6593
                     /gene="modE"
                     /locus_tag="N7A07_001560"
     CDS             5805..6593
                     /gene="modE"
                     /locus_tag="N7A07_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU2476248.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6661..8133
                     /gene="modF"
                     /locus_tag="N7A07_001561"
     CDS             6661..8133
                     /gene="modF"
                     /locus_tag="N7A07_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU2476249.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            8395..9411
                     /gene="galE"
                     /locus_tag="N7A07_001562"
     CDS             8395..9411
                     /gene="galE"
                     /locus_tag="N7A07_001562"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU2476250.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SRACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9421..10467
                     /gene="galT"
                     /locus_tag="N7A07_001563"
     CDS             9421..10467
                     /gene="galT"
                     /locus_tag="N7A07_001563"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU2476251.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10471..11619
                     /gene="galK"
                     /locus_tag="N7A07_001564"
     CDS             10471..11619
                     /gene="galK"
                     /locus_tag="N7A07_001564"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU2476252.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11613..12653
                     /gene="galM"
                     /locus_tag="N7A07_001565"
     CDS             11613..12653
                     /gene="galM"
                     /locus_tag="N7A07_001565"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU2476253.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            12855..13607
                     /gene="gpmA"
                     /locus_tag="N7A07_001566"
     CDS             12855..13607
                     /gene="gpmA"
                     /locus_tag="N7A07_001566"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU2476254.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13773..14825)
                     /gene="aroG"
                     /locus_tag="N7A07_001567"
     CDS             complement(13773..14825)
                     /gene="aroG"
                     /locus_tag="N7A07_001567"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU2476255.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15141..15521
                     /gene="ybgS"
                     /locus_tag="N7A07_001568"
     CDS             15141..15521
                     /gene="ybgS"
                     /locus_tag="N7A07_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU2476256.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15635..16576
                     /gene="zitB"
                     /locus_tag="N7A07_001569"
     CDS             15635..16576
                     /gene="zitB"
                     /locus_tag="N7A07_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU2476257.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16573..17292)
                     /gene="pnuC"
                     /locus_tag="N7A07_001570"
     CDS             complement(16573..17292)
                     /gene="pnuC"
                     /locus_tag="N7A07_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU2476258.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17330..18373)
                     /gene="nadA"
                     /locus_tag="N7A07_001571"
     CDS             complement(17330..18373)
                     /gene="nadA"
                     /locus_tag="N7A07_001571"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU2476259.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18651..18726)
                     /locus_tag="N7A07_001572"
     tRNA            complement(18651..18726)
                     /locus_tag="N7A07_001572"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18691..18693),aa:Lys,seq:ttt)
     gene            complement(18873..18948)
                     /locus_tag="N7A07_001573"
     tRNA            complement(18873..18948)
                     /locus_tag="N7A07_001573"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18913..18915),aa:Lys,seq:ttt)
     gene            complement(18952..19027)
                     /locus_tag="N7A07_001574"
     tRNA            complement(18952..19027)
                     /locus_tag="N7A07_001574"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18992..18994),aa:Val,seq:tac)
     gene            complement(19079..19154)
                     /locus_tag="N7A07_001575"
     tRNA            complement(19079..19154)
                     /locus_tag="N7A07_001575"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19119..19121),aa:Lys,seq:ttt)
     gene            complement(19157..19232)
                     /locus_tag="N7A07_001576"
     tRNA            complement(19157..19232)
                     /locus_tag="N7A07_001576"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19197..19199),aa:Val,seq:tac)
     gene            complement(19268..19343)
                     /locus_tag="N7A07_001577"
     tRNA            complement(19268..19343)
                     /locus_tag="N7A07_001577"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19308..19310),aa:Lys,seq:ttt)
     gene            complement(19508..20299)
                     /gene="cpoB"
                     /locus_tag="N7A07_001578"
     CDS             complement(19508..20299)
                     /gene="cpoB"
                     /locus_tag="N7A07_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU2476260.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20309..20830)
                     /gene="pal"
                     /locus_tag="N7A07_001579"
     CDS             complement(20309..20830)
                     /gene="pal"
                     /locus_tag="N7A07_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU2476261.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(20865..22157)
                     /gene="tolB"
                     /locus_tag="N7A07_001580"
     CDS             complement(20865..22157)
                     /gene="tolB"
                     /locus_tag="N7A07_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU2476262.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22290..23564)
                     /gene="tolA"
                     /locus_tag="N7A07_001581"
     CDS             complement(22290..23564)
                     /gene="tolA"
                     /locus_tag="N7A07_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU2476263.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADK
                     KAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNG
                     ASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQ
                     AALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23629..24057)
                     /gene="tolR"
                     /locus_tag="N7A07_001582"
     CDS             complement(23629..24057)
                     /gene="tolR"
                     /locus_tag="N7A07_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU2476264.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24061..24753)
                     /gene="tolQ"
                     /locus_tag="N7A07_001583"
     CDS             complement(24061..24753)
                     /gene="tolQ"
                     /locus_tag="N7A07_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU2476265.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24750..25154)
                     /gene="ybgC"
                     /locus_tag="N7A07_001584"
     CDS             complement(24750..25154)
                     /gene="ybgC"
                     /locus_tag="N7A07_001584"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU2476266.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25304..25597)
                     /gene="ybgE"
                     /locus_tag="N7A07_001585"
     CDS             complement(25304..25597)
                     /gene="ybgE"
                     /locus_tag="N7A07_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU2476267.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25597..25710)
                     /gene="cydX"
                     /locus_tag="N7A07_001586"
     CDS             complement(25597..25710)
                     /gene="cydX"
                     /locus_tag="N7A07_001586"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU2476268.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25725..26864)
                     /gene="cydB"
                     /locus_tag="N7A07_001587"
     CDS             complement(25725..26864)
                     /gene="cydB"
                     /locus_tag="N7A07_001587"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU2476269.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(26880..28448)
                     /gene="cydA"
                     /locus_tag="N7A07_001588"
     CDS             complement(26880..28448)
                     /gene="cydA"
                     /locus_tag="N7A07_001588"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU2476270.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7087 a         7588 c         7056 g         6840 t
ORIGIN      
        1 aagatttaca tattcaacta tttgagcgta caactcgcaa ggttacgtta acaaaagcag
       61 gaaaaaggtt gctcccagag gcgcgggaat taataaagaa attcgatgaa acgcttttta
      121 atattcgtga tatgaatgct tatcatcgtg gtatggtgac gttagcgtgt attccgaccg
      181 cagtatttta ttttttaccg ctggcaatcg gtaaatttaa tgagctgtat cccaatatta
      241 aagtgcggat tctggaacaa ggcacgaata attgcatgga atcggtgctg tgtaacgaat
      301 cagactttgg cataaacatg aacaacgtca caaattcatc cattgatttt accccactgg
      361 tcaacgagcc gttcgtgctg gcctgtcggc gtgaccatcc attggctaaa aagcagcttg
      421 tagaatggca ggagttagtg ggttacaaaa tgattggcgt acgttcctcc agtggcaacc
      481 gattgttgat agaacaacag ctagccgaca agccctggaa gctggactgg ttttacgaag
      541 tgcgtcatct ttcgacgtca ttaggactgg tagaggcggg actgggaatt tcggcgcttc
      601 ctggtctggc aatgccccat gcgccctatt cttctattat tggtatccca ctggtggaac
      661 ccgttatacg tcggacattg gggattattc gccgcaaaga tgccgtgctt tctccggcag
      721 cagaacgctt ttttgccttg ctgattaatt tgtggactga cgataaagac aatttgtgga
      781 ccaatatcgt cgaacgccag agacatgcgt tacaggagat aggttaatac accggcgcag
      841 gagagactgc gccgggtaaa tcagcggtta gtgtgcgtta accaccaccc acattggtcc
      901 ctgcccgacc gcgtagcggc ctttttcatg cagtagcccc tgctcaccaa caatttcgta
      961 taccgagatg tggtgcgatt tttgcccggc ggcaatcaga tacttaccac ggtgatcgat
     1021 attaaagccg cgcggctggg tttcagttgg ctggaagccc tctttactca acacgctgcc
     1081 atcttccgaa acgctgaaga cagtaatcag gctggcggta cggtcacagg catataagtg
     1141 acgaccatcc ggggtaatat gaatatcagc cgcccaacgg gtgtcggaga agttttccgg
     1201 catcatatcc agcgtctgga cacattcgat attgccgtgc ggatctttca gttcccagac
     1261 atccactgag ctgtttaact cattgacgca atatgcatat tgttcgtttg gatggaatac
     1321 catatgacgc gggccggccc cttcaacggt agtcacttcc gcagggtcct gcgccaccag
     1381 atgaccatca tcgctgaccg taaacaggca aatgcgatcc tgctttaatg ccggaaccca
     1441 cagcgtacgg ttgtccggtg agatattggc ggaatggcaa ccgtccagcc cctcgaccac
     1501 atcgacgacg ccaactggca ggccatcttc cagacgcgtt acgctcacgt tacccgcatt
     1561 gtaagaacct acaaagacaa actgcccctg gtgatcggtg gaaatatgcg tcggactacc
     1621 cggcagcgca gactctgcgg caaaggtcag tgcgccatcg tccggggcga tacgatacgc
     1681 caggacgcga aactcagggc gaacaccaac atagagataa cgtttgtccg ggctgaccac
     1741 catcggctgc acctgccccg gcacatcgac aacctgtgtc agcgtcagtg cgccttcatg
     1801 attcaggttc cagacgtgaa tttgctggct ctcagggctg gcgatataaa ctgtttgctt
     1861 catgaatgct cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc
     1921 ggtgaatgct gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc
     1981 gcagaagaaa attcttaacc ttaatctgga atacgccatg accacacgcg tgattgctct
     2041 cgacttagac ggcaccttat tgaccccgaa aaagaccctg ctaccttcat cgatagaagc
     2101 cctggcccgc gctcgcgaag caggttatca attaatcatc gtcacaggtc gccatcacgt
     2161 cgctattcat cctttttatc aggcgctggc gctggataca cctgctattt gctgtaatgg
     2221 cacctatttg tatgattatc atgcaaaaac cgtgctggaa gcggacccaa tgcccgttaa
     2281 taaagcccta caactcattg agatgctgaa tgaacaccac attcacggtc tgatgtatgt
     2341 cgatgacgag atggtttatg agcacccgac cgggcatgtc attcgcacgt ctaactgggc
     2401 gcaaaccctg ccgccggaac agcgtccgac tttcacacaa gtcgcttctc tggctgaaac
     2461 ggcgcaacaa gttaacgccg tatggaagtt cgccctcacg cacgatgacc tgccgcaatt
     2521 gcagcatttt ggtaagcatg tcgaacatga actgggactg gagtgtgaat ggtcctggca
     2581 cgatcaggtt gatattgcac gcggcggcaa cagcaaaggt aaacgtttga cgaaatgggt
     2641 tgaggcgcaa ggctggtcga tggaaaacgt cgtggcgttc ggcgataact ttaatgatat
     2701 cagtatgctg gaggccgctg gtacaggcgt ggcgatgggc aacgccgatg acgcggtaaa
     2761 agcgcgcgcc aacattgtga ttggtgataa caccaccgac agcattgccc agttcattta
     2821 tagccacctg atttaatcag gcggttatcg acacactttt aatttgcgcg tacagccaca
     2881 ggccaggttt gatcgccagt tcatccctgg cccacgggct gatacgcgcc cacagcgttt
     2941 taccgccgac ttccagttcc acttccacct ggccgttgtc gtcataacta ttaacaactt
     3001 ttgcccgcaa tacgttacga atgctggttt gctgcggcgg ttgtaaaacc agagaaacat
     3061 cggaagcctg aatgcggata cgtagcgcag cttgcagcgg ttcgtccagc ttattgaccc
     3121 acaaatgctg atcgcccagc gccagcgcgg tcatcgcgta atgcggatga tgctcaagta
     3181 ccgtcacttt cagaatgcta ctttgttgct ctttcggcag ccacggattc atcacgctac
     3241 taccccagac ttcttccagc gcgccaaagg ctttcacctg accgttttcc agtaccatca
     3301 ctctgtctgc cagatggagg atctcatcca gcgaatggct gacatacaac atcgggatgt
     3361 tgatttcccg cgtcagacgt tgcagataag gcaacagttc gcgtttacgc ggaatatcca
     3421 gtgacgccag cggttcatcc agcaacagca attctggtgc cgtcagcaaa gcccgcccaa
     3481 tcgctacgcg ctgtttttcg cctccggaca ggctgcctgg taaacggtca agcaacggtt
     3541 caatgcctaa aagcgccacc agcttatcga actgatcgac catgcttttc gccatgccgt
     3601 agcgcagatt gccacgcact ttgtaatgcg ggaacagccg cgcatcctga aaaacatagc
     3661 caacgcgacg cttttccggc gtcaggcaga tacctttttc ggcatcattt agtacccgac
     3721 cattgaggac aatcctccct ttttgcgggc gcgtcagtcc actgatggcg ttaatcagcg
     3781 aagtttttcc ggcaccggag acgccaaaga tagcagtgat gccattggcg ggcagcgttt
     3841 cattaatagt caggcaatgg ttgcccaacg tctgggaaaa attcagttcc agcatgatta
     3901 gcgccccgcc cgttcacggc tgattctggc cagccattct gaaatcaaca gggagatcat
     3961 cgccagcgca atagaaataa tgcacagtct cgctgcgcca ctttccccgc cgggggtctg
     4021 aatcagggta tacatggcag aaggaatggt tcgcgtttca ccaggaatgt tcgacacaaa
     4081 ggtgatggtt gcgccaaatt caccgagaga acgagcaaaa gccagtaccg taccaacaat
     4141 aattcccggt aaggtcagcg gtaacgtgat agtaaagaaa acgcgccagc gcccggcccc
     4201 cagtgttctt gcggcctgtt ccagtttgac atcaacccct tccagcgcca gacgaattgc
     4261 ccgcaccatc agcggaaacg acatgacggc ggcagcgaga accgcgccgc gccagctaaa
     4321 ggcgaaggta ataccaaacc agtcatacag acgttcaccg ataaatccgc gccgccccat
     4381 cgaaactaat aataagtaac cgacgaccac gggcggtaac accagcggta gatgcagtac
     4441 gctgtcgagc agagctttgc ccggaaacgt gcaacgcacc agtaaccagg caaaaaagat
     4501 cccaaacggc aggctaaaca gcacagccag ggaagaaact ttcaggctta ataaaactgc
     4561 ctgccattct ggatcggtca gtatcattac ttggttgtaa atccgtaacg tttaaagatt
     4621 tccgctgcct gcggtccctt cagataatca taaaatgctt tcactgtcgc attgttatgc
     4681 ccttccacaa cagcaaccgg atattccact tttttatgtg aatcttccgg gaaggtggca
     4741 accactttta cccctttgct agcaactgcg tcagaaccgt aaacaatgcc caacggcgct
     4801 tcattgcgtt cgaccaacgc cagcgcgcca cgaacatctt ccgccggagc cagtttcggg
     4861 gagagcgtat cccatgcgcc cagtttttgc agtgcttctt ttgcataaat gccagcggga
     4921 acatgttccg gatcgccaac cgccaggcga ccgccattca gcagtgaagt ccagttggtt
     4981 ttgctgtcga tggtaaaatc tttctgctcg ctggctttcg gtgctacaac gaccaggcta
     5041 ttgccgagca gtgtctgacg cgtagctgta tcgatcgctt ttttatcaac cgcataatcc
     5101 atccatttct gatcggcaga aataaacaga tccgcagggg cacccgcttc aatctgacgg
     5161 gcgagagtag atgacgaagc gaaagaagaa accacatcca cgcctttctc ttttttatac
     5221 tgcgtagcaa tgtcctgcat tgcgttagtc agtgatgcgg cggcgaacac cgtgattttc
     5281 ccttcatctg ccagtgcatt gccagcaaca gcgaaagaga gtgccgcccc agcaaacaag
     5341 ttcaaccatt tacgagccat tggtaacccc ttaatgtaac gttatgtagg cgacaatata
     5401 acgactaact cggaattttc ccagcagtta ttgctaacct ttgtgaggta gataagaaaa
     5461 aatatcggca ggaaaagcag gaagttgaga aaaagaaaat gcccgactaa gcgggcattc
     5521 agggaatcaa tgattttgtc cgggctggtc ttttttacca acaccagaaa agatgttgaa
     5581 tacttcacca agaccgtaaa tcagacccag gatgatggcc atcacgacag gtaccatgat
     5641 tacggcgaat accagacttt ttaataactc taacatggtc aactccagat atagtcatga
     5701 gactattcta accgctaagc acagaaaagc actccccttt tgtgcggtca gctttgcgtg
     5761 gcgttcgttt tccgtcacaa taagactttt gccaggacat tgttatgcag gccgaaatcc
     5821 ttctcaccct taagctccaa caaaaattat tcgccgaccc gcgccgcatt tcgctactaa
     5881 aacacattgc gctttccggt tccattagcc agggagcgaa agatgccggt atcagctata
     5941 aaagcgcctg ggatgccatt aacgagatga atcagttaag tgagcatatt ctggtcgagc
     6001 gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac ccgctatggt cagcgactga
     6061 ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc ctttgatgtg ttaagtgacg
     6121 atgacgccct gccgctgaac agcctgctgg ccgcgatctc acgtttttca ctccaaacca
     6181 gcgcccgtaa ccagtggttc ggtaccatca ccgcccgcga tcatgatgac gttcaacagc
     6241 atgttgatgt cttactggct gatggaaaaa cacgcctgaa agtcgcaatt accgcacaaa
     6301 gcggcgcgcg tctggggctg gatgaaggca aagaagtgtt gatattgcta aaagcgccgt
     6361 gggtaggcat tactcaggac gaagcggtcg cgcaaaacgc tgacaaccaa ttaccgggta
     6421 ttattagtca tattgagcgc ggcgcagagc agtgcgaagt attaatggcg ctaccggacg
     6481 ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac ttctcttcag caaggacaga
     6541 atgtcacggc ctactttaat gccgacagcg tgattatcgc cacactgtgc taagcgtgtt
     6601 gacaatttgt tatgaaacac gtatccctgt cagtaatcgc tgcacaaaat gggatataaa
     6661 atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta gcgacacaaa aacgctgcaa
     6721 ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt ttgtcggttc gaatggaagc
     6781 gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc cgcttttgaa aggtgaacgg
     6841 caaagccagt tttcccacat cactcgtctc tccttcgagc aattgcaaaa gctcgtcagc
     6901 gacgaatggc agcggaataa caccgatatg ctcggccctg gcgaagatga caccggacgc
     6961 actacggctg agattattca ggatgaagta aaggatgcac cgcgctgcat gcaactggca
     7021 cagcagttcg gtattacccc cctcctcgac cgacgcttta aatacctttc cactggcgag
     7081 acgcgaaaaa ccctgctgtg tcaggcgctg atgtcggagc ctgacttgtt gattcttgat
     7141 gagccgttcg atggcctgga tgttgcctca cgtcagcagc tggctgagcg actcgcctcg
     7201 ttacatcagt ccggtattac tctggtactg gtgctcaatc gcttcgatga gatcccggag
     7261 tttgtccagt ttgctggcgt gctggcggat tgcacgttag cggaaactgg cgctaaagag
     7321 gaactgctcc aacaagcact cgtcgcgcaa ctggcgcata gtgaacagct tgaaggtgtg
     7381 caactgccgg agccggatga accttcagca cgtcacgcct tacccgccaa cgaaccgcgc
     7441 attgtgctga ataatggcgt ggtttcttat aacgatcgcc ccattcttaa taaccttagc
     7501 tggcaggtga atccaggtga acactggcaa attgtcgggc caaatggcgc gggaaaatcg
     7561 acgttattaa gcctgattac tggcgatcat ccgcaaggtt acagcaacga tttgacgctt
     7621 ttcggacgac gtcgtggcag cggcgaaacc atctgggata tcaaaaagca tatcggttac
     7681 gtcagcagta gtttgcatct ggattaccgg gtcagtacta ctgtgcgtaa tgtgattctt
     7741 tctggctatt ttgattcgat tggcatttat caggccgttt cggatcgcca gcaaaaactg
     7801 gtgcagcagt ggctggatat tctcggcatt gataaacgca cggctgacgc tccgttccat
     7861 agtctttcct ggggacagca gcgtctggcg ctgatcgtcc gcgcactggt gaaacatccg
     7921 acgttgctta ttctcgacga accattgcag gggcttgatc cgctcaatcg ccagcttatt
     7981 cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc aattgttgtt tgtttcgcac
     8041 catgctgaag atgcgccggc ttgtattacc catcgtctgg agttcgtgcc ggacggtggc
     8101 ctctatcatt atgcgctgac aaaaataaac taagtcggta atgctgactg tgccggatgc
     8161 ggcgtaaacg ctttatccgg cacacggtca gacgcatcca ggcatgaaac tgcgtctttt
     8221 tttcagataa aaagcgcaat cattcacaat ccctctgttt tataatcact taatcgcgca
     8281 taaaaaacgg ctaaattctt gtgtaaacga ttccactaat ttattccatg tcacactttt
     8341 cgcatctttg ttatgctatg gttatttcat accataagcc taatggagcg aattatgaga
     8401 gttctggtca ctggtggtag cggttacatt ggaagtcata cctgtgtgca attactgcaa
     8461 aacggtcatg atgtcatcat tcttgataac ctctgtaaca gtaagcgcag cgtactgcct
     8521 gttatcgagc gtttaggcgg caaacatccg acgtttgttg aaggcgatat ccgtaacgaa
     8581 gcgttgatga ccgagatcct gcacgatcac gctatcgaca ccgtgatcca cttcgccggg
     8641 ctgaaagccg ttggcgaatc ggtacaaaaa ccgctggaat attacgacaa caatgtcaac
     8701 ggtactctgc gcctgattag cgccatgcgc gccgctaacg tcaaaaactt tatttttagc
     8761 tcctccgcca ccgtttatgg cgatcagccc aaaattccat acgttgaaag cttcccgacc
     8821 ggcacaccgc aaagccctta cggcaaaagc aagctgatgg tggaacagat cctcaccgat
     8881 ctgcaaaaag cccagccgga ctggagcatt gccctgctgc gctacttcaa cccggttggc
     8941 gcacatccgt cgggcgatat gggcgaagat ccgcaaggca ttccgaataa ccttatgcca
     9001 tacatcgccc aggttgctgt aggccgtcgc gactcgctgg cgatttttgg taacgattat
     9061 ccgaccgaag acggtactgg cgtacgcgat tacatccacg taatggatct ggcggacggt
     9121 cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg tacacatcta caacctcggt
     9181 gctggcgtag gcagcagcgt gctggacgtg gttaatgcct tcagcagagc ctgcggcaaa
     9241 ccggttaact atcattttgc accgcgtcgc gagggcgacc ttccggccta ctgggcggac
     9301 gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc gcacactcga tgaaatggcg
     9361 caggacacct ggcactggca gtcacgccat ccacagggat atcccgatta aggaacgacc
     9421 atgacgcaat ttaatcccgt tgatcatcca catcgccgct acaaccagct caccgggcaa
     9481 tggattctgg tttcaccgca ccgcgctaag cgcccctggc agggggcgca ggaaacgcca
     9541 gccaaacagg tgttacctgc gcacgatcca gattgcttcc tctgcgcagg taatgtgcgg
     9601 gtgacaggcg ataaaaaccc cgattacacc gggacttacg ttttcactaa tgactttgcg
     9661 gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg atcctttgat gcgttgccag
     9721 agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg atcacagtaa aacgctgcca
     9781 gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct ggcaggagca aaccgcagaa
     9841 ctggggaaaa catacccgtg ggtgcaggtc tttgaaaaca aaggtgcggc gatgggctgc
     9901 tctaacccgc atccgcacgg acagatttgg gcaaatagct tcctgcctaa cgaagctgag
     9961 cgcgaagacc gcctgcaaaa agaatatttt gccgagcaga aatcgccaat gctggtggat
    10021 tatgttcagc gcgagctggc agacggtagc cgtaccgttg tcgaaaccga acactggtta
    10081 gcagttgtgc cttactgggc tgcctggccg ttcgaaacgc tacttctgcc caaagcccac
    10141 gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg atttggcact ggcgttgaaa
    10201 aagctgacca gtcgttatga caacctcttc cagtgctcct tcccctactc tatgggctgg
    10261 cacggcgcac cgtttaatgg cgaagagaat caacactggc agctgcacgc gcacttttat
    10321 ccgcctctgt tgcgctccgc caccgtacgt aaatttatgg ttggttatga aatgctggca
    10381 gaaacccagc gagacctgac cgcagaacag gcagcagagc gtttgcgcgc agtcagcgat
    10441 atccattttc gcgaatccgg agtgtaagaa atgagtctga aagaaaaaac acaatctctg
    10501 tttgccaacg catttggcta ccctgccact cataccattc aggcgcctgg ccgcgtgaat
    10561 ttgattggtg aacacaccga ctacaacgac ggtttcgttc tgccctgcgc gattgattat
    10621 caaaccgtga tcagctgtgc gccacgcgat gaccgtaaag ttcgcgtaat ggcagccgat
    10681 tatgaaaatc agcttgatga gttttccctt gatgcgccca ttgtcgcgca tgaaaactat
    10741 caatgggcga actacgttcg tggcgtggtg aaacatctgc aactgcgtaa caacagcttc
    10801 ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg gtgccgggtt aagttcttcc
    10861 gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc tttatcatct gccgctggac
    10921 ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc agtttgttgg ctgtaactgc
    10981 gggatcatgg atcagctaat ttccgcactc ggcaagaaag atcatgcctt gctgattgac
    11041 tgtcgctcac tggggaccaa agcagtttcc atgccgaaag gtgtggctgt cgtcatcatc
    11101 aacagtaact tcaaacgtac cctggttggc agcgaataca acacccgtcg tgaacagtgc
    11161 gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg atgtcaccat tgaagagttc
    11221 aacgctgttg cacatgagct ggacccaatc gtggcgaaac gcgtgcgtca tatcctgact
    11281 gaaaacgccc gcaccgttga agctgccagc gcgctggagc aaggcgacct gaaacgtatg
    11341 ggcgagttga tggcggagtc tcatgcctct atgcgcgatg atttcgaaat caccgtgccg
    11401 caaattgaca ctctggtaga aatcgtcaaa gctgtgattg gcgacaaagg tggcgtacgc
    11461 atgaccggcg gcggatttgg cggctgtatc gtcgcgctga tcccggaaga gctggtgcct
    11521 gccgtacagc aagctgtcgc agaacaatat gaagcaaaaa caggtattaa agagactttt
    11581 tacgtttgta aaccatcaca aggagcagga cagtgctgaa cgaaactccc gcactggcac
    11641 ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc
    11701 tgatggactg gggtgcgact ttactttcag cccgtattcc gctttccgat ggcagcgtcc
    11761 gcgaggcgct gctcggctgt gccagcccgg aatgctatca ggatcaggcc gcgtttctgg
    11821 gtgcctctat tggtcgttat gccaaccgca tcgccaatag tcgttatacc tttgacggtg
    11881 aaaccgtgac gctttcgcca agtcagggcg ttaaccagct gcacggcggc ccggaagggt
    11941 tcgacaaacg tcgctggcag attgtgaacc agaacgatcg tcaggtgctg tttgccctga
    12001 gttcagatga tggcgatcag ggcttcccgg gtaatctcgg cgcgacggtg caatatcgtc
    12061 tgaccgacga taaccgtatc tccattactt atcgcgccac agttgataaa ccttgcccgg
    12121 tgaatatgac taatcacgtc tatttcaatc ttgacggcaa gcagtctgac gtgcgcaatc
    12181 acaagttgca gattctggcg gacgaatatc tgccggttga tgaaggcggt attccgcacg
    12241 acggcctgaa atctgtcgcc ggaacgtctt ttgatttccg cagcgccaaa atcatcgcca
    12301 gtgagtttct tgccgacgac gatcagcgca aagtgaaagg ttacgatcac gcattcttgt
    12361 tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt ctggtcagca gatgaaaaat
    12421 tgcagctgaa ggtctacacc accgctccgg ctctgcaatt ctactccggc aactacctcg
    12481 gcggcacacc gtcgcgggga accgaacctt acgccgactg gcaagggctg gcgttggaaa
    12541 gcgagtttct gccggacagc ccgaaccacc ctgaatggcc gcaaccggac tgtttcctgc
    12601 atcctggtga agagtattcc agcctgacgg aatatcagtt tattgctgag taatgttaag
    12661 ccctcgtcat gagggcttta tctcatattg ttcaaatcac cagcaaacac cgacatattt
    12721 gcaactcaat attcacaaca accttacact gcgccactat tttcgctatg gttatgcgta
    12781 agcattgctg ttgcttcgtc acggcaatat aatgagaatt attatcatta aaagatgatt
    12841 tgaggagtaa gtatatggct gtaactaagc tggttctggt tcgtcatggc gaaagtcagt
    12901 ggaacaaaga aaaccgtttc accggttggt acgacgtgga tctgtctgag aaaggcgtaa
    12961 gcgaagcaaa agcagcaggt aagctgctga aagaggaagg ttacagcttt gactttgctt
    13021 acacctctgt gctgaaacgc gctatccata ccctgtggaa tgtgctggac gaactggatc
    13081 aggcatggct gcccgttgag aaatcctgga aactgaacga acgtcactac ggtgcgttgc
    13141 agggtctgaa caaagcggaa actgctgaaa aatatggcga cgagcaggtg aaacagtggc
    13201 gtcgtggttt tgcagtgact ccgccggaac tgactaaaga tgatgagcgt tatccgggtc
    13261 acgatccgcg ttacgcgaaa ctgagcgaga aagaactgcc gctgacggaa agcctggcgc
    13321 tgaccattga ccgcgtgatc ccttactgga atgaaaccat tctgccgcgt atgaagagcg
    13381 gtgagcgcgt aatcatcgct gcacacggta actcgttacg tgcgctggtg aaatatctcg
    13441 ataacatgag cgaagaagag attcttgagc tgaatatccc gaccggcgtg ccgctggttt
    13501 atgagtttga cgagaacttc aaaccgctga aacgctatta tctgggtaat gctgacgaga
    13561 tcgcggcgaa agcagcagcc gttgcgaacc agggtaaagc gaagtaaacg tcattcgttt
    13621 aaaaatgaga aagccgactg caagtgagtc ggcttttttg tttgtcgtcg ctaacaatgg
    13681 agcacattgc ctgatgcgac gctgcgcgtc ttgtcagggc tacggtgatt catcggatac
    13741 gccactctga cggcgcatcc gacaattaaa ctttacccgc gacgcgcttt cactgcgttc
    13801 gccagttgac gtaacagagc atcggtatct tcccagccaa tgcaggcatc ggtgatgctc
    13861 ttaccatagg ccagcggctc cccgctctcc aggctctgat tgccttccac cagatgactt
    13921 tccaccatca caccgataat ggccttttcg ccaccggcaa tctgctggca aacgtcagca
    13981 caaacatcca tctgcttttt gaattgtttg gacgaattag catggctgaa atcgatcatc
    14041 acctgagctg gcagacctgc tttgttcagc ccttctttca cttcagcaac gtgcttcgcg
    14101 ctgtagttag gctctttacc gccgcgcaga atgatatggc aatcgccgtt accgctggta
    14161 ttcacaatcg ccgaatgccc ccatttagtt acggacagga agcagtgcgg cgcaccggcg
    14221 gcattaatgg catcgatagc cactttaatc gtaccgtctg tgccattttt aaaacctacc
    14281 ggacaagaaa gaccagacgc cagttcgcgg tgcacctgcg attccgtggt acgtgcacca
    14341 attgcgcccc agctcatcag gtcagcgaga tactgaggag tgatcatatc gaggaattca
    14401 cccgccgctg gcagaccgct gtcgttaata tcaagcagca atttacgggc tatacgcaga
    14461 ccgtcgttga tctggaagct gttatccata tgcggatcgt taatcagccc tttccagccc
    14521 accgtagtac gcggcttttc aaaatagacg cgcattacga tttccagctc atctttcagc
    14581 tcttcacgca gcgccagcaa gcgagtggca tactctttag ccgcgacagg atcatgaatt
    14641 gagcacgggc caatcaccac caacaggcga tcatcattac ctttcaggat cttatggatc
    14701 gcttttcggg catgggcaac cgtattcgcg gcattttcag tagcggggaa tttttccagc
    14761 aatgcgacag gaggaagtaa ctctttgatt tctttgatgc gtaaatcgtc gttctgataa
    14821 ttcatgtctg ttccagtgtt gccatactta tcttagtgaa tgcaatactt ccaatctata
    14881 tcttccgtca gaatgtgtaa acggggtttt acactatgaa cggattaatc ctggaattag
    14941 caagaaaaac gccagattgt cgcgaaaaac gagatctctc ctacaatttc taactgtaac
    15001 tcctttgttt gttaattatt tcaagattct ctgctggatt tctttgcctg gctgaaatct
    15061 taaaccaatg ccttatattc acctgcaaat gcactgttgg aagaggttat ccgacataac
    15121 gaccataaca ggagcatcct atgaaaatga caaaactggc cacactattt ctgactgcca
    15181 ctctaagcct tgccagcggt gccgcactgg ctgccgatag cggagcgcaa accaataacg
    15241 gccaggcaaa cgccgcagct gatgcgggcc aggtagcccc tgacgcccgt gaaaatgtcg
    15301 cgccaaataa cgtcgacaat aacggggtaa ataccggttc tggcggcact atgctgcatc
    15361 cggatggttc ttcaatgaac aatgacggca tgaccaaaga tgaagagcac aagaacacca
    15421 tgtgcaaaga tggtcgctgc ccggatatca ataaaaaggt acaaaccggt gatggcatca
    15481 ataatgatgt cgataccaaa accgacggta ccacgcagta accctgcaaa atagccaata
    15541 atgggagagc ggagtctctc ccttttaatt tttcccttct aaaagcagta tgatgtcata
    15601 aagttaagtg agaataatta agggatgact cgtcatggcg cactcacact cacacacgtc
    15661 ctcacacctg ccagaagata ataatgctcg tcgcttgttg tatgctttcg gcgtgactgc
    15721 cgggtttatg ctggtagaag tcattggtgg ttttctttcc ggttctctgg ctttgctggc
    15781 cgatgcgggt catatgttga ccgatactgc cgccctgctt ttcgccctac tcgccgtgca
    15841 attttcacgt cgtcctccca ccattcgcca cactttcggt tggctaagat tgaccacgct
    15901 cgcggcgttt gtgaatgcca tcgccctcgt ggtaattacc attttgattg tctgggaggc
    15961 gatagaacgc ttccgcacgc cgcgtccggt cgagggcggc atgatgatgg caattgccgt
    16021 ggctgggttg ctggcaaata tactttcttt ctggttactt catcacggca gcgaagagaa
    16081 aaacctcaac gtacgagcag cggcactgca tgtgctcggg gacctgctgg gttcggttgg
    16141 ggctattatt gccgcgttga ttattatctg gaccggctgg acacctgctg accccattct
    16201 ctcgatactg gtgtcgcttc tggttctgcg tagcgcgtgg cgactgttga aagatagcgt
    16261 gaatgaatta cttgagggtg caccggtatc gctggatatc gctgaactga agcgtcgtat
    16321 gtgccgggaa atcccggaag tccgcaatgt gcaccatgtg catgtatgga tggtaggcga
    16381 gaagccggtg atgacgttgc atgtgcaggt gatcccgcca cacgatcacg atgccttgtt
    16441 ggatcagatc caacactacc tgatggatca ctatcagatt gagcacgcca ccattcagat
    16501 ggaatatcaa ccttgtcatg ggccagactg ccacctgaat gaaggggtgt ctggccattc
    16561 acatcatcac cattaatggg acagcgcgcg tgagcctctt tcgcgtgcgc tattgatcca
    16621 catccggctg ccgttgagcg caataaaggt caggatgatg tactccagag acatggcgta
    16681 aacgccctga agtgcaaaaa taacgacgct aatcacgtta ataatcaccc acaacagcca
    16741 gttttccaca tacttacgag tcatcagaat cattgccacg atagataaca ccatcatgca
    16801 agaatcccag aacgggaaag cgtccggttg tagttcaggc atcgcaacct gtaatcctaa
    16861 tgcttgcatg atgatgactg ccacgcgggt caaaaatgca aacaccggat tgataaatac
    16921 cgtcatcaga ccaatcgaaa caacgcaaac cgccaaccag ctgagtgcct tcggcaatgg
    16981 caaccagcga atttttaact ccgcctcgtt ctgactggtt tgtcgcgacc acgcatacca
    17041 gccgtaaata ttcgcggcaa agaaaaacac ctgtaatagc aggctggcat acagctgaat
    17101 ctgaaagaaa ataatgccaa acaaggtgac gttgatcagg ccaaagaagt agttgctgat
    17161 cttctccaga ctggcaaggc caatacacag caacccggcg attgtgccta ccgcttcgat
    17221 ccatgagaga tcataaccgc ctgctcctat tggtatatgt accaggatat tctgcacact
    17281 aaaaaaatcc atctttcccc caaagcgtaa cgttacgcct tattattcgt tatccacgta
    17341 gtgtagccgc aaaatccagc atacgattga gcggcaccag cgccctctct cgcagccttt
    17401 catcaacatg aacctcgtaa ttgcttcctt cctgttctaa tgcctcagcg atggcctgga
    17461 ggccattcat ggccatccac ggacaatgcg cgcagctgcg gcaggttgcg ccctcacccg
    17521 cggttggtgc ttccagtaac tctttatctg gcaccgcctg ctgcattttg tagaaaatac
    17581 cccgatcggt tgccacaata agcctctgat gtggcaatgt tttcgcagca gcgatcagtt
    17641 gactggtgga accgaccgca tccgccatat cgacaatagc ttgtggtgat tctggatgca
    17701 ccagtatggc agcatccggg tattcttctt gcaagcgggt taacgcctga gtcttaaact
    17761 catcatgcac aatgcaggca ccctgccagc ataaaatgtc tgcgcccgtc tttttttgca
    17821 cgtaacgccc cagatgtttg tcgggtgccc agatgatttt ttcacccaaa ctatccagat
    17881 gatcaataag ttcgacggca atgcttgaag ttaccaccca atctgcccgc gcttttaccg
    17941 cagcagaagt gttggcatag acgacgacag tacgatcggg atgggcatcg caaaatgcgt
    18001 taaattcttc aacagggcag ccgagatcca gtgaacactc agcctgaagt gtcggcatca
    18061 gaattgtttt ttccggactg agaattttgg cggtttctcc cataaatctc accccagcga
    18121 ctaacaacgt agaagcggga tgctttgcac cgaagcgcgc catttccaga gaatcagaaa
    18181 tacagccacc ggtttcttct gccagttgtt gaatttcggg atcggtatag tagtgggcaa
    18241 ccatcaccgc attacgttct tttagcagac gttttatctt ctcgcggtaa tacgcttttt
    18301 catcaatgct taacggcgtc ggcttcgggg ggaaaggata aatcgccgtg tctggatcaa
    18361 acatcacgct catcttacca tctcgtttta caggcttaac gttaaaaccg acattagcgt
    18421 gatattcgct gttatcggtg taatttgttt tatatgctaa acaagatagt cgaatggtgg
    18481 ggaaaagtca ctcgaattac gtatgagatg ccgaatgcca cactttgcgt cttattcctc
    18541 cgacgtaaag ttgtttacgc agcaagcgcg gagtatgtca tcagggtcaa gaattgaaat
    18601 tgagagatgc ctgaatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg
    18661 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    18721 cgacccatac gggtgttacc tgaaagattt ctactcggaa ccaatcttta aaattggtgg
    18781 gtcgtgcagg atgactcgct tcgcttgccc tacgggccgt tgctgacgca acgttatcct
    18841 tcacgcttaa catctgagtt tgatgttaaa attggtgggt cgtgcaggat tcgaacctgc
    18901 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccga gtggtgggtg
    18961 atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta
    19021 atcacccgat actgcgctgg atactacaaa ctacttccac cgaacccacc gtaattagtg
    19081 gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac
    19141 tgagctaacg acccggtggt gggtgatgac gggatcgaac cgccgacccc ctccttgtaa
    19201 gggaggtgct ctcccagctg agctaatcac ccgatattac gctggatact acaactaact
    19261 aggttagtgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc
    19321 tctaccaact gagctaacga cccacttttt cgttgctttc ggtttgtttg atatcccgtg
    19381 gcaacggcgg catatattac tgatttcaga cttgagcgca acaaaaattt cgatgtagat
    19441 cactcaactg cttatgattc gcacgacacg accagaaata atgcgacttc tggtcgtgtg
    19501 ttatgcatta catcgcgttc agacgttttt gtgcctgttt agcgccatca gtaccagggt
    19561 atttactgat aacctgctgg tatacggctt tcgcttttgc ggtgtcacct ttgtcctgca
    19621 tgatgacgcc gactttaaac atcgcatctg cagcctttgg tgacttcgga tagtttttca
    19681 ctaccgaagc aaaatagtac gccgcatcat cttttttacc cttgttgtag tttaactgac
    19741 ccagccaata attggcgttt ggcaggtaag ttgaatcagg gtaatttttg atgaaattct
    19801 gaaatgccac cattgcgtca tcctggcggg atttatcctg caccagcgca atagctgcat
    19861 tgtaatccgt gtttgcatca ccgcttttca ccggcgcgcc agcattcgca gtaccagcat
    19921 cagctgtcgg cgtcgttgat gccgccgcac cgctttgatc accgctggtt gattgcgccg
    19981 ctgcaccacc gctgctgagg ctgtcgatct gcaacaggat ctgcttttgc cgctccacga
    20041 cctgattcag ttgatactga ttttcctgaa tctgaccacg cagggaatca atatcggatt
    20101 gattatcaga gagttgttgc tggagttggg ttaaaagctg gctgtgagca ttagaaatac
    20161 gctcaagttg aatgacgcgg tcttcgaccg agcctgagcc gacactactg attggtgcct
    20221 gagcaaaagc ggcccagggg gccgctatac caaccagtaa cgacagactc aatagttgat
    20281 gtctgaagtt actgctcatg caattctctt agtaaaccag taccgcacga cggtttttgg
    20341 agtatgccgc ttcgtcatga cccagtactg caggtttttc tttaccgtaa gaaacgatgg
    20401 agatctggtc tgcagaaacg cctttaccct gcaggtacat cttaacggcg ttcgcacgac
    20461 gttcacccag ggagatgttg tattccggag taccacgttc gtccgcgtga ccttctacgg
    20521 tgactttgta agacgggttg ctacgcagga agtttgcatg tgcatccagc atttgagcga
    20581 agtcagaacg gatatcgtac ttgtccagat cgaagtaaac gatgttgttc tgctgcagct
    20641 gttgcatttg cagacgagcc tgctcttcgg aagacatgtt gccgttgccg ccgttcgcat
    20701 ccataccagt gccggcaccc agcatgcctt cgctgccgtc attgctggcg ttcttgttgg
    20761 aagaacatgc cgcaattgcc ataacaggca gagcaatcat cagccctttc agcactttgt
    20821 tcagttgcat ttcaatgatt cctttactat tcaattaatt attatcacag atacggcgac
    20881 caggcaggga atttgacctg tccatcagtt gccggaagac gcgctttgaa acgcccatct
    20941 gtagaaacca aattcagcac ggatcccatc ccctgagaag agctgtagat taccatagtg
    21001 ccgttaggtg ccagacttgg cgtttcatcc aggaacgtgg acgacagaac ttgtacgcct
    21061 cccgttgcca gatcttgttt ggcaatgtgc tgctgcccac cattggagct gaccattacc
    21121 ataaatttac cgtcgctgct gacatccgca tcctggttct gcgaaccttc ccaggtaata
    21181 cgttgtggcg caccgccgtt gatattcact ttataaacct gtggacgacc ggcctggtca
    21241 gaagtaaatg ccaggttctg gctgtccggg aaccaggtcg gttcggtatt gttactgcga
    21301 ccatcagtca cctggcggat ctgaccagaa gccaaatcca ttacgtacag gttcaggcta
    21361 ccggttttcg acaaggcgaa tgccagtttg ctgccgtctg gcgagaatgc aggcgcaccg
    21421 ttgtgacgcg ggaatgaagc cacctgacgt acagcgccgt ttgccagcgt ttgtataacc
    21481 agcgcggaac gaccgctttc gaaggtcaca taagccagtt tagaaccgtc tggtgaccac
    21541 gccggggaca tcagcggctg tggtgaacgg tgaacgacaa actggttgta accgtcatag
    21601 tcagatacgc gcagttcata cgggaactga ccgccgttgg tctgaacaac gtaggcaata
    21661 cgggtacgga acgcaccttt aataccggtc agtttttcaa acacttcatc actggcggta
    21721 tgaccagcat aacgcagcca ctgcttgttc actttgtacg agttctgagc aagtacagta
    21781 cccggtgcgc cgccagtgtc aacaagttga taagcaacat tgtaagaacc atccggattc
    21841 ggagtgacct gaccgacaac tacagcgtca atacccagcg cggaccatgc agctggttgt
    21901 acttcctgcg cactacccgg ctgctgtggc agacgagcgc gatctaacgg attaaattta
    21961 ccgctgttac gcaagtcggc agcaacgatg ccgccaatat cttcaggtgc cgcaccaggg
    22021 cccgcccact ggaaaggaac aactccaata ggacgaccgg aatctacacc gctgtcgatc
    22081 acaatgcgga cttcagcatg cagaactgat gcccacagta tgagaaaacc aaatgctact
    22141 cgtaatgcct gcttcatcat atctccctta tctggaccga tggcccacga taatttagca
    22201 gaatgttaac aaactaaaat acacaaaact accagaaccc cgtggcaacc ggtgcctgat
    22261 gttgaccgtc ggaacagtca acatcgcgat tacggtttga agtccaatgg cgcgtttttg
    22321 aacacttcat atactgcctg gcttggtggt ttcgggatct tcgcaagttt agctgctgcc
    22381 aacgcagcct gacaaagtgc gggatcgcca ccttcaggtt tgatatccag taacatacca
    22441 tcgggtgcca gttttatgcg cagcgtacag gttttgcctg cataggacga tgcgtcatag
    22501 aacttacttt cgatagcaga tttaatctgc ccggcatagt tattgatatc ggcccctgat
    22561 gcgccattgt ttttagtatt accactcccg gcaggcgaag cattgttccc tttcgcccct
    22621 ccccccgttt tcggtgcatt cttaccagag cttagctcac cgaaaatatc atctgcctcc
    22681 gcggcagctt ttgctgcagc ggctttttca gctgctgctt ttgcagctgc cgcttttttg
    22741 tctgcggcgg ctttttctgc cgctgctttc ttatcagctg ctgccttttc agcagccgct
    22801 ttttctgctg cttttttgtc ggctgcagct ttctctgctg ctgccttttc agcagccgct
    22861 ttttctgctg cttttttgtc ggctgcagct ttctctgctg cttttttgtc ggctgcagct
    22921 ttctctgctg ccgctttctt tcttgcttca gctgcagctg cttctgccgc ttccgctttc
    22981 ttcttcagcg ccgcagctgc tgcctcggct tttttctgcg cttcggctgc ggctttggcg
    23041 gcttctgctt ctgctttttt cttcgcgtct gcagccgctt tcttcgctgc ttcttccgca
    23101 gctttatcat ccgcttcggc cttcgcttta gcatctgccg ccgctttcgc tgccgcctct
    23161 tccgcttgct tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgt
    23221 tcctgagcag ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt
    23281 ttctcacgca gttcttcagc agcctgctgt tccttcatct tgcgctgctc atcagaacgc
    23341 ttcgcgcttg attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca
    23401 accatgacag cgtcgatgga cgaaccaccg ccgcctccag ctgaagcttc tatattctca
    23461 tcgaacgaac tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt
    23521 atcgcccgct tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa
    23581 ctgttcgcct gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg
    23641 cgtcattaaa ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat
    23701 ttcatcgtaa ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt
    23761 gaaacggctg gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt
    23821 ctcaaccacc acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc
    23881 gttactgctc accgcctgtg attcagtagc gtctggcaaa tcgacctcca cgctctgggt
    23941 gatgatgggc gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg
    24001 tacaatgttg atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc
    24061 ttaccccttg ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc
    24121 ttccataaag ttgtcgtaat tcagttccag tttgtttaca cgttggttga gacggttata
    24181 agccattacc gccggaatag cggcgaacag accaattgcc gtcgcaatca acgcttctgc
    24241 gataccgggc gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc
    24301 gtgcatgatc ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt
    24361 accgaggaac ggaatgtgcg tttccagatt ttcaagttcg cggttcatgg aaatacgcat
    24421 cgcacgcgac gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg
    24481 cacaaattct ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt
    24541 cccctggctc tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa
    24601 cgcttcggct tcgcgcgccg ctgcgttaag aatacgggtc cgctggataa taatggccca
    24661 ggatgcgatt gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt
    24721 caggaacaaa tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg
    24781 ggaagcgcac gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca
    24841 ttcagcaaag tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc
    24901 attgatgtta tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat
    24961 tccaccgtca ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgttgactg
    25021 aagtgatgat gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg
    25081 tggtacacca caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac
    25141 agcgttgtat tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc
    25201 tactatacgc gcgcgattga ggtttgggaa tgagggcaag ttaagggagc gtaaaaattt
    25261 atgggcctgg cacggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa
    25321 tcagcccgac aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa
    25381 ccttctgcgg acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca
    25441 ataaaccatg ccagatttcc agttcactgg tcttcgcggc gaaacgagat gggtcccaaa
    25501 acatacatcc tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt
    25561 ccattaccgc atacaaggtt gcgataatct tactcatcag atgtcttctt gaccggcttt
    25621 gcctgattcg acgtgctcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag
    25681 cgttcccaga atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg
    25741 gtgttacgtt caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg
    25801 gtgtagagca gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagtgtc
    25861 agctggctgg aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc
    25921 atcacaaacg ggaacattgc gataccggca gtcaggatga tgcaggccag cgtcagggag
    25981 gagaacacaa acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcggc
    26041 agaaccacac ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc
    26101 catgcgccag cttcacgaac cacttcttta ttcagcgggt tagaggctgc gtaatggtcc
    26161 attgtcgatt tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg
    26221 aaacagacca gtgtcaccag tgccgccacc tgagccgttg cacgggtacg caggtgcagt
    26281 tcgcccacgg tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc
    26341 accacgcctg ccagcaggcc gaacgggtta agcagctgga agaagttacc ggtgtagtac
    26401 agacgcagat attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca
    26461 ccaatcacca acggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc
    26521 cagcgggttt cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac
    26581 gccagcacga ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc
    26641 caggcagcaa acagtgcgcc gcctgcggtg atcagccaaa cctggttacc gtcccagtgt
    26701 ggtgcgatgg agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg
    26761 agcatgccca cccccatgtc gaaaccatca gtgactgcaa aaccaatcag cagaacgcca
    26821 accagcagcc accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct
    26881 tagcgtgccg gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg
    26941 ctgcttgggc cgaggcgtgc aaacttgaac attaagaaca attctgccac caggaacagg
    27001 gtatacaggc cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgag
    27061 ttcgccacag ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc
    27121 acgaaccagc ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc
    27181 aggagccatt ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg
    27241 attgccagca gcaggaagcc acacgccacc atgatacgga acgcgaagta cagcggcgct
    27301 acacgcggaa tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg
    27361 tttggcgtat agcgtttcag cagcagaccg taaccgaggt ccttcttcat gctattgaac
    27421 tggtcacgaa ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc
    27481 ttcatcccgt tacgaatgcg ttcttcatgc tgcaccatca gttctttcag gccgataact
    27541 ggagtatcca cggaacgcgt tgcaattatg cccagcgcgt aagggatctg gatcgcaaat
    27601 ttgttcgtct cctcttcctg atcaggaatg ccgaacagag taaaggcagc aggcgcaggt
    27661 tgcgtttccc actcagcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg
    27721 tagccggatt catcacccag aacaataaca gacagaacgg cagccatacc gaagctggca
    27781 gcgatagcaa aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg
    27841 ctgataccga ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat
    27901 ttcacctgag caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata
    27961 gtttcaaagt tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc
    28021 cacagtgcgg acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact
    28081 ttacccagac gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag
    28141 gccatcagac cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag
    28201 taagaccagt tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg
    28261 aagttgatac caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg
    28321 gaaaggacgt agaccgtttc cataatggcc agcaggaacg ccataccgag cgtcagtggc
    28381 acaaaaagga agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata
    28441 tctaacatca tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa
    28501 gggtcacacg catgccccaa tacaaaatta tttgctcgcc tctatcgttg ccatttcatc
    28561 aaaagaggaa a
//
LOCUS       DBFIFS010000039        28402 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.039, whole genome shotgun sequence.
ACCESSION   DBFIFS010000039 DBFIFS010000000
VERSION     DBFIFS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.039"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..579
                     /locus_tag="N7A07_001589"
     CDS             <1..579
                     /locus_tag="N7A07_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476271.1"
                     /translation="ILYFANVFFNPLALSFIIATIICITIKKRSSRSFIRGTCWLMGL
                     FLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGPTLVTRDAHAILAIVA
                     FSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGVQTMSDGKVRHAIFMTLLD
                     NTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            702..1328
                     /locus_tag="N7A07_001590"
     CDS             702..1328
                     /locus_tag="N7A07_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476272.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1367..1612)
                     /gene="zapB"
                     /locus_tag="N7A07_001591"
     CDS             complement(1367..1612)
                     /gene="zapB"
                     /locus_tag="N7A07_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU2476273.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2038..2883
                     /gene="glpF"
                     /locus_tag="N7A07_001592"
     CDS             2038..2883
                     /gene="glpF"
                     /locus_tag="N7A07_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU2476274.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2906..4414
                     /gene="glpK"
                     /locus_tag="N7A07_001593"
     CDS             2906..4414
                     /gene="glpK"
                     /locus_tag="N7A07_001593"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU2476275.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4644..5654
                     /gene="glpX"
                     /locus_tag="N7A07_001594"
     CDS             4644..5654
                     /gene="glpX"
                     /locus_tag="N7A07_001594"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU2476276.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5751..6497
                     /gene="fpr"
                     /locus_tag="N7A07_001595"
     CDS             5751..6497
                     /gene="fpr"
                     /locus_tag="N7A07_001595"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU2476277.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6502..6930)
                     /gene="uspD"
                     /locus_tag="N7A07_001596"
     CDS             complement(6502..6930)
                     /gene="uspD"
                     /locus_tag="N7A07_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU2476278.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6957..7256)
                     /gene="yiiS"
                     /locus_tag="N7A07_001597"
     CDS             complement(6957..7256)
                     /gene="yiiS"
                     /locus_tag="N7A07_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU2476279.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7468..7908)
                     /gene="yiiR"
                     /locus_tag="N7A07_001598"
     CDS             complement(7468..7908)
                     /gene="yiiR"
                     /locus_tag="N7A07_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU2476280.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8009..8608
                     /gene="yiiQ"
                     /locus_tag="N7A07_001599"
     CDS             8009..8608
                     /gene="yiiQ"
                     /locus_tag="N7A07_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU2476281.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8716..9483
                     /gene="tpiA"
                     /locus_tag="N7A07_001600"
     CDS             8716..9483
                     /gene="tpiA"
                     /locus_tag="N7A07_001600"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU2476282.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9538..10293)
                     /gene="cdh"
                     /locus_tag="N7A07_001601"
     CDS             complement(9538..10293)
                     /gene="cdh"
                     /locus_tag="N7A07_001601"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU2476283.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10400..11389)
                     /gene="sbp"
                     /locus_tag="N7A07_001602"
     CDS             complement(10400..11389)
                     /gene="sbp"
                     /locus_tag="N7A07_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU2476284.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11708..12670)
                     /gene="pfkA"
                     /locus_tag="N7A07_001603"
     CDS             complement(11708..12670)
                     /gene="pfkA"
                     /locus_tag="N7A07_001603"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU2476285.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12851..13753)
                     /gene="fieF"
                     /locus_tag="N7A07_001604"
     CDS             complement(12851..13753)
                     /gene="fieF"
                     /locus_tag="N7A07_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU2476286.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13902..14402)
                     /gene="cpxP"
                     /locus_tag="N7A07_001605"
     CDS             complement(13902..14402)
                     /gene="cpxP"
                     /locus_tag="N7A07_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU2476287.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14552..15250
                     /gene="cpxR"
                     /locus_tag="N7A07_001606"
     CDS             14552..15250
                     /gene="cpxR"
                     /locus_tag="N7A07_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU2476288.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15247..16620
                     /gene="cpxA"
                     /locus_tag="N7A07_001607"
     CDS             15247..16620
                     /gene="cpxA"
                     /locus_tag="N7A07_001607"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU2476289.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16726..17400)
                     /gene="yiiM"
                     /locus_tag="N7A07_001608"
     CDS             complement(16726..17400)
                     /gene="yiiM"
                     /locus_tag="N7A07_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU2476290.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17549..18532)
                     /gene="kdgT"
                     /locus_tag="N7A07_001609"
     CDS             complement(17549..18532)
                     /gene="kdgT"
                     /locus_tag="N7A07_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU2476291.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18792..19412)
                     /gene="sodA"
                     /locus_tag="N7A07_001610"
     CDS             complement(18792..19412)
                     /gene="sodA"
                     /locus_tag="N7A07_001610"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU2476292.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19697..20731
                     /gene="rhaT"
                     /locus_tag="N7A07_001611"
                     /pseudo
     CDS             19697..20731
                     /gene="rhaT"
                     /locus_tag="N7A07_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(20728..21576)
                     /gene="rhaR"
                     /locus_tag="N7A07_001612"
     CDS             complement(20728..21576)
                     /gene="rhaR"
                     /locus_tag="N7A07_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU2476293.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21650..22486)
                     /gene="rhaS"
                     /locus_tag="N7A07_001613"
     CDS             complement(21650..22486)
                     /gene="rhaS"
                     /locus_tag="N7A07_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU2476294.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22774..24243
                     /gene="rhaB"
                     /locus_tag="N7A07_001614"
     CDS             22774..24243
                     /gene="rhaB"
                     /locus_tag="N7A07_001614"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU2476295.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            24240..25499
                     /gene="rhaA"
                     /locus_tag="N7A07_001615"
     CDS             24240..25499
                     /gene="rhaA"
                     /locus_tag="N7A07_001615"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU2476296.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            25674..26498
                     /gene="rhaD"
                     /locus_tag="N7A07_001616"
     CDS             25674..26498
                     /gene="rhaD"
                     /locus_tag="N7A07_001616"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU2476297.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            26508..26822
                     /gene="rhaM"
                     /locus_tag="N7A07_001617"
     CDS             26508..26822
                     /gene="rhaM"
                     /locus_tag="N7A07_001617"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU2476298.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(26863..28257)
                     /locus_tag="N7A07_001618"
     CDS             complement(26863..28257)
                     /locus_tag="N7A07_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU2476299.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         7066 a         7276 c         7094 g         6966 t
ORIGIN      
        1 attttatatt ttgctaacgt attttttaac ccacttgcct tatcatttat tatcgccaca
       61 atcatctgta taacgataaa aaaaagaagc agccgcagct ttattcgcgg cacttgttgg
      121 ttaatgggat tatttcttat ctatagcagc tatacggtgt gggaacgaca cagcatatgg
      181 gattatactt tcccggagcc aggtatcacc gtcacggttc cgagcaaaca gtgggttgcc
      241 aatatagtaa aacaaggacc aaccctggtg acccgggatg cccatgctat tttagctatt
      301 gttgccttct cacaaaatga actcggtgtg cattcaatcg aggaacttac cgcgactcaa
      361 aacggaactg cagaaatgca tgtttgtgat gttcagggat ttgcctgcgc ttatcaggaa
      421 ggagtgcaaa caatgagtga tggcaaagta agacatgcta ttttcatgac tctactggat
      481 aacactaatt tgatacaact ggtcgcagct atcgacccag attatttgga cgaatatcaa
      541 gaagaagtaa tgaaaatgat gctttctgct cgtcaatgat ctgatgtgca tgttaataat
      601 aatggattga tatgagatac gttatcttat taatactatg gataataact gtttttctta
      661 gtcatcttca gcacagtaac ttgcttactg gagatccagg gatggttggc gagtatatcg
      721 gttcagtact tcttggaccg ttattacttc ccgtgcttgt gtcaggtatt ctgtgcacat
      781 tcactaaaaa aacgcgtaac ttcgccagtt ttactcgcgg gtgctgttgg gtattgggag
      841 tcctgctttt atccaacgtc ggcaatacgt ttcgtctgtt caccccgtgg cactatacgt
      901 ttgagaaggc agctatttcc gtgactgtcc caaaccgaca ctggaatacg gtttctatta
      961 gtactgataa aaccattgat ataagaagtg aagataatag cgtctttatt tctgctttcc
     1021 gcctgcccgc aggacgtagc gccgatgact cgctggaaga gttaaagaag atgcagcgag
     1081 acaatctcaa ggatcaatac aatgaagaaa ccttccagtt tcatgattgt aatgccaaac
     1141 atttcacctg taagtatcag gatgtgttga taaattttga tggtcagcag aaaagaacca
     1201 tcagtgttta tcttgaagac acccccaggg ctgtggggat cattgcatta atggagccag
     1261 atacggcaga taaatatcgc caacaggcga tggagattat gttgtctgct aaaaatacag
     1321 tgaagtaaaa aataaggcgc cttgcggcgc ctctgcgtgg aagagattaa acctcttcca
     1381 tgcgacccag cagggcctgc agacgttcct gccagccgtt ctgctgttct ttcagatggt
     1441 tgttctcacg ctccagctct tcgcgctgat gctgggcatt ttgaacttcc tgcgacaatg
     1501 agttgttttt ttctttcagc tcttcgattt ccatctgcaa cagagtgatg gtatcaatcg
     1561 cctgctgtac ttttgcttcc agtttctcaa acacttctaa tgacattgtc atacctctcc
     1621 tgaattgcaa ggcgttgatg gataaaaaat cctcgtcccg attaccggtg acgccttaat
     1681 aaatacgagc gcactttagt tagctccgat tgtatgaagc cgcgccatcg ctgtccagcg
     1741 gcacgccttg cagattacgg tttgccacac ttttcatcct tctcctggtg acataatcca
     1801 caccaatcga aaatgttaat aaatttgttg cgcgaatgtt ctaacaaaca tgcatcatgt
     1861 acaatcagat ggaataaatg gcgcgataac gctcatttta tgacgaagca cacacatttt
     1921 aagttcgata tttctcgttt ttgctcgtta acgataagtt tacagcatgc ctacaagcat
     1981 cgtggaggtc cgtgactttc acgcatacaa caaacattaa ctcttcagga tccgattatg
     2041 agtcaaacat caaccttgaa aggccagtgc attgctgaat tcctcggtac cgggttgttg
     2101 atttttttcg gtgtgggttg cgttgcagca ctaaaagtcg ctggtgcgtc ttttggtcag
     2161 tgggaaatca gtgtcatttg gggactgggg gtggcaatgg ccatctacct gaccgcaggg
     2221 gtttccggcg cgcatcttaa tcccgctgtt accattgcat tgtggctgtt tgcctgtttc
     2281 gacaagcgca aagttattcc ttttatcgtt tcacaagttg ccggcgcttt ctgtgctgcg
     2341 gctttagttt acgggcttta ctacaattta tttttcgact tcgagcagac tcatcacatt
     2401 gttcgcggca gcgttgaaag tgttgatctg gcaggcactt tctctactta ccctaatcct
     2461 catatcaatt ttgtgcaggc tttcgcagtt gagatggtga ttaccgctat tctgatgggg
     2521 ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac gcggcccttt ggctcccttg
     2581 ctgattggtc tactgattgc ggtcattggc gcatctatgg gcccattgac gggttttgcc
     2641 atgaacccag cgcgtgactt cggtccgaaa gtctttgcct ggctggcggg ctggggcaat
     2701 gtcgccttta ccggcggcag agacattcct tacttcctgg tgccgctttt tggccctatc
     2761 gttggcgcga ttgtaggtgc atttgcctac cgcaaactga ttggtcgcca tttgccttgc
     2821 gatatctgtg ttgtggaaga aaaggagacc acaactcctt cagaacaaaa agcttcgctg
     2881 taatatgact acgggacaat taaacatgac tgaaaaaaaa tatatcgttg cgctcgacca
     2941 gggcaccacc agctcccgcg cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc
     3001 gcagcgcgaa tttgagcaaa tctacccaaa accaggctgg gtagaacacg acccaatgga
     3061 aatctgggcc acccaaagct ccacgctggt agaagtgctg gcgaaagccg atatcagttc
     3121 cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa accactattg tctgggaaaa
     3181 agaaaccggc aagcctatct ataacgccat tgtctggcag tgccgtcgta ccgcagaaat
     3241 ctgcgagcat ttaaaacgtg acggtttaga agattatatc cgcagcaata ccggtctggt
     3301 gattgacccg tacttttctg gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc
     3361 tcgcgagcgt gcgcgtcgtg gtgaattgct gtttggtacg gttgatacgt ggcttatctg
     3421 gaaaatgact cagggccgtg tccatgtgac cgattacacc aacgcctctc gtaccatgtt
     3481 gttcaacatc cataccctgg actgggacga caaaatgctg gaagtgctgg atattccgcg
     3541 cgagatgctg ccagaagtgc gtcgttcttc cgaagtgtac ggtcagacta acattggcgg
     3601 caaaggcggc acgcgtattc caatctccgg gatcgccggt gaccagcagg ccgcgctgtt
     3661 tggtcagttg tgcgtgaaag aagggatggc gaagaacacc tatggcactg gctgctttat
     3721 gctgatgaac actggcgaga aagcggtgaa atcagaaaac ggcctactga ccaccatcgc
     3781 ctgcggcccg actggcgaag tgaactatgc gttggaaggt gcggtgttta tggcaggcgc
     3841 atccattcag tggctgcgcg atgagatgaa gttgattaac gacgcctacg attccgaata
     3901 tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg gttccggcat ttaccgggct
     3961 gggtgcgccg tactgggacc cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt
     4021 gaacgctaac cacattatac gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt
     4081 gctggaagcg atgcaggccg actctggtat ccgtctgcac gccctgcgcg tggatggtgg
     4141 cgcagtagca aacaatttcc tgatgcagtt ccagtctgat attctcggca cccgcgttga
     4201 gcgcccggaa gttcgcgaag tcaccgcatt gggtgcggcc tatctcgcag gcctggcggt
     4261 tggcttctgg cagaacctcg acgagctgca agagaaagcg gtgattgagc gcgagttccg
     4321 tccaggcatc gaaaccactg agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa
     4381 acgcgcgatg gcgtgggaag aacacgacga gtaatgtcag gcacctgaat cgattgcctg
     4441 atgctgatcg cctgatgcga cgcttacgcg tcttatcagg cctacgagcc aatgccattt
     4501 tgtgtaggcg ggataaggcg ttcacgccgc atccggcatc acgcagactc tgaaaccgtg
     4561 cgggggcaac cccgcacaca tcaataatcc cacccttccc ctgtgctaca cttcgcgcca
     4621 ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt tcccgcgtca
     4681 ccgaatctgc ggcgctggct ggctacaaat ggttaggacg cggcgataaa aacaccgcgg
     4741 acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt gacggcacca
     4801 tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt gaaaaagtcg
     4861 gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc acgcgcatga
     4921 cggcgatggg tcaggctaac tcgctggcgg tgctggcagt aggcgataaa ggctgtttcc
     4981 tcaacgcgcc agatatgtat atggagaagc tgatcgtcgg accgggagcc aaaggcacca
     5041 ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt cgcggcggcg ctcggcaaac
     5101 cgttgagcga actgacggta acgattctgg ctaaaccacg ccacgatgcc gttatcgctg
     5161 aaatgcagca actcggcgta cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa
     5221 ttctcacctg tatgccagac agcgaagttg acgtgctgta cggaattggt ggcgcgccgg
     5281 aaggcgtggt ttctgcggcg gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc
     5341 tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc gagcaggagc
     5401 tggcacgctg caaagcgatg ggcatcgaat ccggtaaagt attgcgtctg ggcgatatgg
     5461 cgcgcagcga taacgtcatc ttctctgcca ccggtattac caaaggcgat ctgctggaag
     5521 gcattagccg caaaggcaat atcgcgacta ccgaaacgct gctgatccgc ggcaaatcac
     5581 gcaccattcg ccgcattcag tctatccact atctggatcg caaagacccg gaaatgcagg
     5641 tgcacatcct ctgattgatt tgatcgattg agccttccag tccttcggga ctggaatttt
     5701 tttgttcgga gaacgaagat aaggcaattc aatcaaaaca ggagaaaaac atggctgatt
     5761 gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt agtctcaccg
     5821 ttcacgcccc tgtgcatccg tttaccgccg ggcaatttac caagcttggc cttgaaatcg
     5881 acggcgaacg cgtccaacgc gcctactcct atgttaactc gcccgataat cccgatctgg
     5941 agttttacct ggtcaccgtc cccgatggca aattaagccc acgattggcg gcactgaaac
     6001 cgggcgatga agtgcaggtg gttagcgaag cagctggctt ctttgttctg gatgaagtac
     6061 cagattgcga aacgctatgg atgctggcaa ccggtacagc gattggccct tatttatcga
     6121 ttctgcaact aggtaaagat ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac
     6181 gttatgccgc cgacttaagc tatttgccac tgatgcaaga actggaaaaa cgctacgaag
     6241 gcaaactgcg cattcagacg gtggtcagtc gggaaacggc agcggggtcg ctcaccggac
     6301 ggataccggc attaattgaa agtggtgaac tggaaagcgc gattggcctg ccgatgaata
     6361 aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat acgcaacagt
     6421 tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggt cacatgacag
     6481 cggagcatta ctggtaagcg gttacttatc gataaacggc acgatgagca aatccgcact
     6541 caacttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg agtggtgatg
     6601 accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg tttccggcat
     6661 ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg tcagtttata
     6721 cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg cagggaagta
     6781 gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca ggtgagcgtc
     6841 attatgtctg gcgagctcca aggctttatt caccagtaag gcatcttctt cattcccgga
     6901 aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca actccatcaa
     6961 gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga gaaggtgaag
     7021 gccgcatcaa gctcaacttg cccctccaga tccttaatct tataggccac gctggcccct
     7081 tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa ggactctgct
     7141 tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc
     7201 gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc tttcataggg
     7261 cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc cgttaaggga
     7321 agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgcg taaggtgaag
     7381 agttcagaag gattttccgg gtattgtcat ttgcatcgtg gaaataacaa tcccggaaaa
     7441 aaatagcagt aagagatata ttaaaggtta attacttgat ttattgtcgg ctttatactt
     7501 cacatcctga gtatctttac catatttatt ttcgccttgt gtgccaacaa acgcaccaag
     7561 atcgataagc atcatcacca gaatcaacgt cgggacaaaa cgccctaccg cccattgcca
     7621 gacacccggt aaaatcgccc agttacccgc cagtagcatc cacgccacaa tcatcagaaa
     7681 tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta acggttactg ccgctgttgg
     7741 ccagagcaag cacacaaggc aaaacgccgc ggtctgaata tcgagtaaat ttttacccgc
     7801 cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg
     7861 gcgtccaata cgccctttaa atgagaataa ccattgctgt atggtcatgt aagttccttg
     7921 atggttgtct tttccaggat tctacccttt tgacaagggc gacagatatc gttttaatcg
     7981 gagccagtca taacaaaagg tactgtcaat gaagccaggg tgtacgctgt tttttctctt
     8041 atgttctgca ttaaccgtta caacaacggc gcatgcacaa acaccagata cggcaacgac
     8101 cgcgccttat ctgctggctg gagcccctac tttcgatctc tccatcagcc agtttcgaga
     8161 agactttaac agccagaatc ccagcctgcc actgaacgaa tttcgtgcca tcgacagcag
     8221 tcccgacaaa gccaatctca ctcgtgctgc cagtaaaatt aacgagaact tgtatgcttc
     8281 tacagcgctg gagcgcggta ccttaaaaat caaaagcatt caaatgacat ggctacccat
     8341 ccaggggcca gagcaaaaag ccgcgaaagc gaaagctcag gaatacatgg cagcggtgat
     8401 ccgcacactc accccattaa tgaccaaaac acaaagccag aaaaaactgc agtcgctact
     8461 aacggcgggg aaaaacaaac gttattacac cgagacagaa ggtgcactgc gctatgttgt
     8521 cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc tggcgctatc
     8581 tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc
     8641 tatactgttt cacagacctg ctgccctgcg gggcggccat cttcctttat tcgcttataa
     8701 gcgtggagaa ttaaaatgcg acatccttta gtgatgggta actggaaact gaacggcagc
     8761 cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag agctggcagg tgttgctggc
     8821 tgtgcggttg caatcgcacc accggaaatg tacatcgata tggcgaagcg tgaagctgaa
     8881 ggcagccaca tcatgctggg tgcgcaaaac gtggacctga acctgtccgg cgcattcacc
     8941 ggtgaaacct ctgctgctat gctgaaagac atcggcgcac agtacatcat catcggtcac
     9001 tctgaacgtc gtacttacca caaagaatct gacgaactga tcgcgaaaaa attcgcggtg
     9061 ctgaaagagc agggcctgac tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa
     9121 gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg cggtactgaa aactcagggt
     9181 gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat cggtactggc
     9241 aaatctgcaa ctccggctca ggcacaggct gttcacaaat tcattcgtga ccacatcgct
     9301 aaagttgacg ctaacatcgc tgaacaagtg atcattcagt acggcggctc tgtaaacgcg
     9361 tctaacgctg cagaactgtt tgctcagcca gacatcgacg gcgcgctggt tggcggtgcg
     9421 tctctgaaag ctgacgcttt cgcagtaatc gttaaagctg cagaagcggc taaacaggct
     9481 taagtctgac aggtgccgga tttcatatcc ggcacttact ttccttaact cttcgcctta
     9541 acgcaaaatc tcacactgat gatcctgaat ttcctcggct gaagcacggt taagcgtcag
     9601 tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca ctctgccgta ccatcgccag
     9661 cccgtagctt cccatatgtt cccgcgcctc aggtacttct tctgccagca tcataaatgg
     9721 gctgcgttgt accagttcgc tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa
     9781 accacctggc agtggcaacc agcggctgct gatgttcgcc agattgttat ccagctgttc
     9841 gcgcacatca ggacgaatac aagagatatg aatatgaaaa tggttttgcg tacgcccggt
     9901 gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc ggctggccgt attttttgct
     9961 cataaaatca cgcgcctgcc aggccaacca aaagaagttc ggcgttgaag gatcggtcaa
    10021 caaaggactt tcagtaccgt taatacgata cgttggcatc aacagatatt gcagtgggcc
    10081 attaagatct tttaaaacca cgtatccggc attgggtttg acttccgcac atggcgaagg
    10141 attttgattt tgctgctgat tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc
    10201 cgactcttca ccggttaatt tccagtaacc aataccggca gccacaacgg cgataactat
    10261 catcaccaaa aaaagaagac ccgctttttt catctttttt tccctgtacc tcaaagagat
    10321 gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt cgcgagaaac caccgggttt
    10381 gataattatc ctggggaaat cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa
    10441 aatgctcttt ttgcgctttc gtccagccgc cgaactcttc atcaatggtg aataacttca
    10501 gctttggaaa cgcattttcg tactttttcg ccacctcagc gtcgcgcgga cggtagtagt
    10561 ttttcgcggc aatttcctga ccttctggcg agtagagata tttcaggtag gcttccgcca
    10621 cctctttagt accttttttc tcgaccactt tatcgaccac cgacacggtt ggctctgcga
    10681 ggatagactc actcggcgtg acgatttcga atttatcttt ccccagttca ttcgctgcca
    10741 gcagagcttc gttttcccag gaaatcagta catcgccaat tccgcgctcg acaaaagtgt
    10801 tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt tttatacagt gcccgaacaa
    10861 aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag cgcgtagccc caggctgcca
    10921 ggtagttcca gcgcgcgcca ccagagcttt tcggattagg cgtgatcacc gaaacaccca
    10981 gtttaatcag atcgttccag tcatggatct gcttcggatt tcccttacgt accaggaaaa
    11041 caatggtgga agtgtacggt gcggagttat ccggcagacg tttgatccac tctttatcaa
    11101 tccgcccgcg ttccgcaatc gcgtccacgt cataggccag agccagcgtg acaacatcag
    11161 cttcaatacc gttgattacc gacgttgctt gtttgcctga accaccgtgt gactgacgaa
    11221 tcaccacgtt atcgccagtt tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt
    11281 cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc tttgccataa
    11341 cgctggttgc cgccagcaaa aatgttaacc ctacgcccca cttgttcatc gcccgactct
    11401 cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa
    11461 aaaaagattg gctataactt gcgggtatat gttgggggtt aaaaaggcgg aagaaatccg
    11521 cctcatattg ctgacaaagt gcgctttatc gatgccggat gcggcgcgaa cgccttatcc
    11581 ggcctacaaa agtttgtaaa ttcaataaat tgtagaattc acgtaggcct gataagcgaa
    11641 gcgcatcagg catttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc
    11701 gaaatcatta gtacagtttt ttcgcgcagt ccagccagtc gcctttgaac ggacgcttca
    11761 tgttttcgat agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgt atacctacgc
    11821 aacgaccgcc gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca
    11881 gaatacggtc gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc
    11941 gggtttcacg accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat
    12001 gttcggtaat cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt
    12061 ttaccaggtc ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg
    12121 caatggccgc agccagcgtc agatcgccac aataacggcc catcacttcc accacggaaa
    12181 tacgctggtg agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca
    12241 gcgcagtgaa gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg
    12301 gcaggccgat gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt
    12361 caccgccgat aaccaccagc gcatcgatcc cacgtttttt caggttttcg atagccacgg
    12421 cgcggatgtt ctcgtcgcgg aattccggga aacgcgcaga accgaggaac gtaccgccac
    12481 ggttgatcat gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac
    12541 ccagatagcc gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa
    12601 ccccgcgaat tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt
    12661 tcttaatcat gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct
    12721 tcttgcacat cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga
    12781 attgattcag gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct
    12841 tttatactga ttatgaaagc atagaccgtt taccctccct aggtacgacg gaacaggggt
    12901 cctgatggat aatcacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat
    12961 ccgccaccat atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa
    13021 taaagcgggt cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc
    13081 aggaagtcac gatatcaata atttcttgcc gttcctcatc aggtaatgcg cgatccagta
    13141 atgactgtac cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc
    13201 ccaatgcaaa cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca
    13261 gaattgcgcc gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct
    13321 ggctttgcgt ccggcgcacc acccaacgct gaaacgagac aaggataatc gtacaaatta
    13381 gcgccacaat tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatatca
    13441 gatgttgaat gcctgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg
    13501 ccagcgccgc gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt
    13561 gcagggaata tcgcaccacc aataaattcg tcaacgacgc gccgatatcc accagcgaat
    13621 ccaccagcgc ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca
    13681 gcaatagcag tgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat
    13741 aagattgatt cataaatact cccgctatca actgacgcta gtataacgga agcaaatcat
    13801 ctgcaatgca ttaagcagca ggcaaattga ggataaaaaa aacccccaca tcatgtgggg
    13861 gaagacaggg atggtgtcta tggcaaggaa aacagggttt actactggga acgtgagttg
    13921 ctactactca atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc
    13981 tccattcgtt gttgatgttt ctcgtttaaa accgcttgct gctccggcgt taacaggcga
    14041 tacatttggt tgcggacttt tgccatctca acctgacgag caatttgctc attcgccatt
    14101 ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac aagacgatgc
    14161 attgtctcca gttcgctaac attaacagga ggctgttcgt gccgggcctg ttgcataaga
    14221 tctcgcatct gctgacgctg atgttcggtt aaacttatgc cgtcgaacat atggctctgc
    14281 gtactgcgct gcgtaagttc ttcacccgga tgccagttat cgcctgaacc gacttcagca
    14341 gcgtggctta atgaactgac tgccagcgtt gaggccatga cggcagcggt aactatgcgc
    14401 atcatttgct cccaaaatct ttctgtcgcg attcaacgat agagagttta cgattcaggc
    14461 tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca tggattagcg acgtctgatg
    14521 acgtaatttc tgcctcggag gtatttaaac aatgaataaa atcctgttag ttgatgatga
    14581 ccgagagctg acttccctat taaaggagct gctcgagatg gaaggcttca acgtgattgt
    14641 tgcccacgat ggggaacagg cgcttgatct tctggacgac agcattgatt tacttttgct
    14701 tgacgtaatg atgccgaaga aaaatggtat cgacacatta aaagcacttc gccagacaca
    14761 ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct
    14821 tgagctgggc gcagatgact atctcccgaa accgtttaat gatcgtgagc tggtggcacg
    14881 tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg
    14941 ttcaccgaca ctggaagttg atgccttagt actgaatcca ggccgtcagg aagccagctt
    15001 cgacgggcaa acgctggagt taaccggtac tgagtttacc ctgctctatt tgctggcaca
    15061 gcatctgggt caggtggttt cccgtgaaca tttaagccag gaagtgctgg gcaaacgcct
    15121 gacgcctttc gaccgcgcta tcgatatgca catttccaac ctgcgtcgta aactgccgga
    15181 tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt cgcggctacc tgatggtttc
    15241 tgcttcatga taggcagctt aaccgcgcgc atcttcgcca tcttctggct gacgctggcg
    15301 ctggtgttga tgttggtttt gatgttaccc aagctcgatt cacgccagat gaccgagctt
    15361 ctggatagcg aacagcgtca gggtctgatg attgagcagc atgttgaagc ggagctggcg
    15421 aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg
    15481 gcaccgccag gacagcgttt gttattggtg accaccgaag gccgcgtgat cggcgctgaa
    15541 cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg ccgataacgc cgatcatccg
    15601 cagaagaaaa agtatggccg cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa
    15661 gataattacc aactttatct gattcgtccg gccagcagtt ctcaatccga tttcattaac
    15721 ttactgtttg accgcccgct attactgctg attgtcacca tgttggtcag tacgccgctg
    15781 ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat
    15841 gaagttgccc agggaaactt acgccagcac ccggaactgg aagcggggcc acaggaattc
    15901 cttgccgcag gtgccagttt taaccagatg gtcaccgcgc tggagcgcat gatgacctct
    15961 cagcagcgtc tgctttctga tatctctcac gagctgcgca ccccgctgac gcgtctgcaa
    16021 ctgggtacgg cgttactgcg ccgtcgtagt ggtgaaagca aggaactgga gcgtattgaa
    16081 accgaagcgc aacgtctgga cagcatgatt aacgacctgt tggtgatgtc acgtaatcag
    16141 caaaaaaacg cgctggttag cgagaccatc aaagccaatc agttgtggag tgaagtgctg
    16201 gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt tgacagttaa cttcccgcct
    16261 gggccgtggc cgctgtacgg caacccgaac gccctggaga gtgcgctgga aaacattgtt
    16321 cgtaatgccc tgcgttattc ccatacgaag attgaagtgg gctttgcggt agataaagac
    16381 ggtatcacca ttacggtgga cgacgatggt cctggcgtta gcccggaaga tcgcgaacag
    16441 attttccgtc cgttctatcg gaccgatgaa gcgcgcgatc gtgaatctgg cggtacaggt
    16501 ttgggactgg cgattgttga aaccgccatt cagcagcatc gtggctgggt gaaagcagaa
    16561 gacagcccgc tgggcggttt acggctggtg atttggttgc cgctgtataa gcggagttaa
    16621 actccgcatt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg agcaggatgc
    16681 ctgatgcgac gctgatagcg tcttatcagg cctacactcc gggggttatt ttccccacaa
    16741 ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc ttttgcatcg ttcgcgtcca
    16801 gcttttcgat aacccggcag cggagagtaa acggtgatac tgatcgtcat caaacggcat
    16861 atgccatgcg atggcagcag cctcctgcac ggtcacatcg ctgacacggg aaaccaactc
    16921 cagcggcgcg tccgcagaca ctttccctgg cgctatcacg ctatacaacc atcccacttt
    16981 acccgtgttt tgcatcaact gcgctatatc gctgatatca aaatggtaat tgagtttata
    17041 gcaaggcgag cgcggctggc tgacctgaat taatgcctct ccccagcgga aaatatcgcc
    17101 aatataaaca ttactttccg tgagaccgtc ggttgagaga ttttcgccaa acgcaggcgc
    17161 aacaaacaac tccgcctgtt ccggaaattc ccgcgcccag tagagataat gctcacgagg
    17221 ataatgacac agcgctctgt ctggtccgcc gtgaaccttt ttctccgcct gctcgtcacc
    17281 ttccagcccc agctccgtta gcatcaactc accatcaacc tggattttgg caattgcgct
    17341 gggtttgcct tcgggataag cctgaatctt gcctgtgtat acatcaaccg gatatcgcat
    17401 ctgttccctc tccacgagaa atttccccat tagaccatat ttattttatg tcaggcgtgc
    17461 tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa accggacgaa cgttagttca
    17521 tccggctgtg gggattaagg aagcaggttt atttcaccgt acctaaaatt tcgattttcg
    17581 ccgctctggc tttgacttta cgtgaccaga tagaggtaag aattggcacc agaatcgaag
    17641 tcacaatgac tgccgtcgct accagcgaag ttgctgccgg agccatcggt ttaaacgcag
    17701 gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc ccctgcggaa ctggaagcgg
    17761 caattccggc agtgccatcg ccaccgccaa tcaatttatc ggcgataatc aacggaatac
    17821 cggtcacgat aattactgcc acacccaaca ggatccccag caaacctgtc tgggcaatta
    17881 cagtcaaatc aatggtattg cctagtgcga aggcaaagaa tggaatcagc gtctgcaccg
    17941 ctttgctgaa aaattctcgt aattcagggt caaggttccc aagggcaaag cccaccagga
    18001 acggtaatac tgcgccgacg aaaacatgcg gttcaaacga ggcaatcccg gcagtgccca
    18061 gaataatcat cgtcatgagc ggcccggact ccaacgacat caacacaaat gccccagctt
    18121 cttcttttgt gccgtactgc tgcatgatgg aagcgtaaag tccgccgttg gtcatatcca
    18181 tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa tccaacttca acaccatgtt
    18241 ccggaataat gcgcgaggca attgccgcaa ccacccacgc gacggcaatt ttagttacca
    18301 ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct taattttatt gacgccccca
    18361 tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt aatcatcccg ttggtgaatg
    18421 atccaaaata tttccccgcc cccggcgaga aggtgtggca cagtgcgcca aggaatagcg
    18481 ggacgagcat catccccccc gggattttct caatcaagcg ttttatctgc atttccatca
    18541 cctgtcggca tatttttaag ggctgatgcg taattcctca atggagcatc agtgttgatg
    18601 gaaacgaaga tagacattca tttaatcaat aaaagtgatc aaaaccacat caactgaaac
    18661 gctgttttat ttttatcgga tcattgtttt tattttgaaa ttgatcacaa aaaaaccacc
    18721 gccattggcg atggttcatt ttttaagctg atatgcggcg taaacgcctc actgcagcag
    18781 acggcaaatg attatttttt cgccgcgaaa cgtgccgctg cttcgtccca gttcaccacg
    18841 ttccagaact ctttgatgta gtccgggcgg cggttctgga atttcaggta gtaagcatgc
    18901 tcccacacat ccaggcccag aatcgggaag ccggatgcgc cagaaatagc ttcacccatc
    18961 agcggggagt cctggttagc agtagaaacc accgccagtt tatcgccttt cagcaccagc
    19021 catgcccagc cggaaccaaa gcgggaagct gccgctttct caaattctgc tttgaagtta
    19081 tctacggaac cgaaatcacg ttcgatagcc gctttcaggt caccctgcag ggtggtgcct
    19141 tttttcagac ctttccagaa caggctgtgg ttagcgtgac cgccagcgtt gttgcgcagt
    19201 acggttttct tgtctgctgg cagctggtcc agtttggtga tcaactcttc aaccggcagg
    19261 ttggcaaatt ctggcaggct ttccaacgcc gcgttggcgt tgttgacgta ggtctgatgg
    19321 tgtttggtgt ggtggatttc catggtctgc ttatcgaagt gcggttccag ggcatcgtaa
    19381 gcatacggca gggatggcag ggtatagctc atattcatct ccagtattgt cgggcggccg
    19441 attgttaatg ccgcgtaagc agttggttca ttatagttaa ttaaatgata ttgaaaatga
    19501 ttatcaatgc cgtacttttc gtaagggtat ggttttgcag gaaaatgccc gagatgtgaa
    19561 gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga caacggcggc aacaggcgaa
    19621 aggttaatcg acagcacgat ttttacactc atctcgtcgg agatgtgacg cgacgaaaaa
    19681 tgatgaggat aagaagatga gtaacgcgat tacgatgggg atattttggc atttgatcgg
    19741 cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa gtaaaaaaat ggtcatggga
    19801 aaccatgtgg tcagtcggtg ggattgtttc gtggattatt ctgccgtagg ccatcagcgc
    19861 cctgttacta ccgaatttct gggcgtatta cagctcgttt agtctctcta cgctgctgcc
    19921 tgtttttctg ttcggcgcta tgtgggggat cggtaatatc aactacggcc tgaccatgcg
    19981 ttatctcggc atgtcgatgg gaattggcat cgccattggc attacgttga ttgtcggtac
    20041 gctgatgacg ccaattatca acggcaattt cgatgtgttg attaacaccg aaggcggacg
    20101 catgacgttg ctcggcgttc tggtggcgct gattggcgta gggattgtaa ctcgagccgg
    20161 gcagttgaaa gagcgcaaga tgggcattaa agccgaagag ttcaatctga aaaaaggact
    20221 ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg tcctttgcga tgaacgccgc
    20281 aaaaccgatg catgaagccg ctgccgcact aggcgtcgat ccactgtatg tcgctctgcc
    20341 aagctatgtt gtcatcatgg gcggcggcgc gatcatcaac ctcggtttct gttttattcg
    20401 tctggcaaaa gtgaaggatt tatcgctaaa agccgacttc tcgctggcaa aaccgctgat
    20461 cattcacaat gtgttactct cagcactggg cgggttgatg tggtatctgc aattcttttt
    20521 ctatgcctgg ggccacgccc gcattccggc gcagtatgac tacatcagtt ggatgctgca
    20581 tatgagtttc tatgtattgt gcggcggtat cgtcgggctg gtgctgaaag agtggaacaa
    20641 tgcaggacgc cgtccggtaa cggtgttgag cctcggttgt gtggtgatta ttgttgccgc
    20701 caacatcgtt ggcatgggca tggcgaatta atctttctgc gaattgagat gacgccactg
    20761 gctgggcgtc atcccggttt cccgggtaaa caccaccgaa aaatagttac tatcttcaaa
    20821 gccacattcg gtcgaaatat cactgattaa caggcggcta tgctggagaa gatattgcgc
    20881 atgacacact ctgacctgcc gcagatattg attgatggtc attccagtct gctggcgaaa
    20941 ttgctgacgc aaaacgcgct cactgcacga tgcctcatca caaaatttat ccagcgcaaa
    21001 gggacttttc aggctagccg ccaaccgggt aatcagctta tccagcaacg tttcgctgga
    21061 tgttggcggc aacgaatcac tggtgtaacg atggcgattc agcaacatca ccaactgccc
    21121 gaacagcaac tctgccattt cgttagcaaa cggcacatgc tgactacttt catgctcaag
    21181 ctgaccgata acctgccgcg cctgtgccat ccccatgcta cctaagcgcc agtgtggttg
    21241 ccctgcgctg gcgctaaatc ccggaatcgc cccctgccag tcaagattca gcttcagccg
    21301 ctccgggcaa taaataatat tctgcaaaac cagatcgtta acggaagcgt aggagtgttt
    21361 atcgtcagca tgaatgtaaa agagatcgcc acgggtaatg cgataagggc gatcgttgag
    21421 tacatgcaga ccattaccgc gccagacaat caccagctca caaaaatcat gtgtatgttc
    21481 agcaaagaca tcttgcggat aacggtcagc cacagcgact gcctgctggt cgctggcaaa
    21541 aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg gctacctcgg ccagagaacg
    21601 aaattgatta ttcgcaatat ggcgtacaaa tacgttgaga agattcgcgt tattgcagaa
    21661 agccatcccg tccctgacga atatcacgcg gtgaccagtt aaactctcgg cgaaaaagcg
    21721 tcgaaaagtg gttactgtcg ctgaatccgc aatgataggc gatgtcagta acgctggcct
    21781 cgctgtgacg tagcagatgt cgggctttca tcagtcgcag gcgattcagg tatcgctgag
    21841 gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt acgcagtgaa agagaaaatt
    21901 gatccgccac ggcatcccaa ttcacctcat cggcaaaatg gtcctccagc caggccagaa
    21961 gcaagttgag acgtgatgcg ctgttttcca ggttctcctg caaactgctt ttacgcagca
    22021 agagcagtaa ttgcataaac aagatctcgc gactggcggt cgagggtaaa tcattttccc
    22081 cttcctgctg ttccatctgt gcaaccagct gtcgcacctg ctgcaatacg ctgtggttaa
    22141 cgcgccagtg agacggatac tgcccatcca gctcttgtgg cagcaactga ttcagtccgg
    22201 cgagaaactg aaatcgatcc ggcgagcgat acagcacatt agtcagacac agattatcgg
    22261 tatgttcata cagatgccga tcatggtcgc gcacaaaaca gaccgtgcca ccggtgatgg
    22321 tatagggctg cccattaaac acatgaatac ccgtgccatg ttcgacaatc acaatttcat
    22381 gaaaatcatg atgatgttca ggaaaatccg cctgcgggag ccggggttct atcgccacgg
    22441 acgcgttacc agacggaaaa aaatccacac tatgtaatac ggtcatactg gcctcctgat
    22501 gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc ttaccttaaa ttttcgacgg
    22561 aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt gaattttcag gaaatgcggt
    22621 gagcatcaca tcaccacaat tcagcaaatt gtgaacatca tcacgttcat ctttccctgg
    22681 ttgccaatgg cccattttcc tgtcagtaac gagaaggtcg cgaattcagg cgctttttag
    22741 actggtcgta atgaaattca gcaggatcac attatgacct ttcgcaattg tgtcgccgtc
    22801 gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt acgagcgtga atgccgcagc
    22861 ctgacgctgc gcgaaatcca tcgttttaac aatgggctgc atagtcagaa cggctatgtc
    22921 acctgggatg tggatagcct tgaaagtgcc attcgccttg gattaaacaa ggtgtgcgag
    22981 gaagggattc gtatcgatag cattgggatt gatacctggg gcgtggactt tgtgctgctc
    23041 gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc gcgatagccg caccaatggc
    23101 ctaatggcgc aggcacaaca acaactcggc aaacgcgata tttatcaacg tagcggcatc
    23161 cagtttctgc ccttcaatac gctttatcag ttgcgtgcgc tgacggagca acaacctgaa
    23221 cttattccac acattgctca cgctctgctg atgccggatt acttcagtta tcgcctgacc
    23281 ggcaagatga actgggaata taccaacgcc acgaccacgc aactggtcaa tatcaatagc
    23341 gacgactggg acgagtcgct actggcgtgg agcggggcca acaaagcctg gtttggtcgc
    23401 ccgacgcatc cgggtaatgt cataggtcac tggatttgcc cgcagggtaa tgagattcca
    23461 gtggtcgccg ttgccagcca tgataccgcc agcgcggtta tcgcctcgcc gttaaacggt
    23521 tcacgtgctg cttatctctc ttctggcacc tggtcattga tgggcttcga aagccagacg
    23581 ccatttacca atgacacggc actggcagcc aacatcacca atgaaggcgg ggcggaaggt
    23641 cgctatcggg tgctgaaaaa tattatgggc ttatggctgc ttcagcgagt gcttcaggag
    23701 cggcaaatca acgatcttcc ggcgcttatc tccgcgacac aggcacttcc ggcttgccgc
    23761 ttcattatca atcccaatga cgatcgcttt attaatcctg agacgatgtg cagcgaaatt
    23821 caggctgcgt gtcgggaaac ggcgcaaccg atcccggaaa gtgatgctga actggcgcgc
    23881 tgcattttcg acagtctggc gctgctgtat gccgatgtgt tgcatgagct ggcgcagctg
    23941 cgcggtgaag atttctcgca actgcatatt gtcggcggag gctgccagaa cacgctgctc
    24001 aaccagctat gcgccgatgc ctgcggtatt cgggtgatcg ccgggcctgt tgaagcctcg
    24061 acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa tgtggatgat
    24121 ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct ttacccctaa tcctgacagt
    24181 gaaattgccc actatgtggc gcggattcac tctacacgac agacaaagga gctttgcgca
    24241 tgaccactca actggaacag gcctgggagc tggcgaaaca gcgtttcgcg gcagtaggga
    24301 ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc cgtttcaatg cactgctggc
    24361 agggtgatga tgtttccggt tttgaaaacc cggaaggttc gctgaccgga gggattcagg
    24421 ctactggcaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc gatctggaac
    24481 aggctatgcg gctgattccg gggccaaaac ggcttaattt acatgccatc tatctggaat
    24541 cagacacgcc agtctcgcgc gaccagatca aacctgagca cttcaaaaac tgggttgaat
    24601 gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgtttctcg catccgctaa
    24661 gcgccgatgg ctttacgctt tcccatgccg acgacagcat tcgccagttc tggattgatc
    24721 actgcaaggc cagccgccgc gtttcggcct attttggtga gcaactcggc acaccgtcgg
    24781 tgatgaacat ctggatcccg gatggcatga aagatatcac cgttgaccgt ctcgctccgc
    24841 gccagcgtct gctggcagct ctggatgagg tgatcagcga gaagctggat ccggcgcacc
    24901 atatcgacgc cgttgagagc aaattgtttg gcattggcgc agagagctac acggttggct
    24961 ccaatgagtt ttacatgggg tatgccacca gccgccagac tgcgctgtgc ctggacgccg
    25021 ggcatttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg ctgtatgtgc
    25081 cgcagttgct gctgcacgtc agccgtccgg ttcgctggga cagcgatcac gtagtgctgc
    25141 tggatgatga aacccaggcg attgccagtg agattgttcg tcacgatctg tttgaccggg
    25201 tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg tattgctgcg tggatcattg
    25261 gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct ggaacctacc gctgagctgc
    25321 gcaagctgga agcggcgggc gattacactg cgcgtctggc actgctggaa gagcagaaat
    25381 cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg tcacgatacg ccagcaggta
    25441 gcgaatggct ggagagcgtg cgggcatatg agaaagaaac tttgagtcgc cgcgggtaaa
    25501 cactgccgga tgcggcacaa gtgccttatc cggcctacag gccggcaata gttgtaggcc
    25561 tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac gagcgcctta
    25621 tccggcctac gggtcgtgca tctgacaaca ccgaatttac aggaacacag aacatgcaaa
    25681 acattaccca gtcctggttt gtccagggaa tgatcaaagc caccaccgat gcctggctga
    25741 aaggctggga cgagcgcaac ggcggcaacc tgacgctgcg cctggatgac gccgatatcg
    25801 cgctttatca cgacaacttc cacccacaac cgcgctatat tccgctcagc caacccatgc
    25861 ctttactggc aaatacgccg tttattgtca ccggctccgg caaattcttc cgtaacgtcc
    25921 agctcgatcc tgcagctaac ttaggcgtcg taaaagtcga cagcgatggc gcgggctacc
    25981 atattctctg ggggttaacc aacgaagccg ttccaacttc tgaacttccg gctcactttc
    26041 tctcccactg cgagcgcatt aaagccacca acggtaaaga tcgggtgatc atgcactgcc
    26101 acgccaccaa cctgatcgcc ctcacctatg tacttgaaaa cgacaccgcg gtcttcactc
    26161 gccaactgtg ggaaggcagc accgagtgtc tggtggtatt cccggatggc gttggcattc
    26221 tgccgtggat ggtacccggc acggacgaaa tcggccaggc aactgcccaa gagatgcaaa
    26281 aacattcgct ggtgttgtgg cccttccacg gtgtgttcgg ttccggaccg acgctggatg
    26341 aaaccttcgg tttaatcgac accgcagaaa aatcagcgga agtgttagtg aaggtgtatt
    26401 cgatgggcgg catgaaacag actatcagcc gtgaagagtt gatagcgctc ggccagcgtt
    26461 tcggcgttac accactcgcc agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag
    26521 cctttgtcat gcaggtaaac cccgacgccc acgaagagta tcagcgtcgg cataatccca
    26581 tctggccgga actggaagca gtgttgaaat ctcacggtgc gcataactac gccatttacc
    26641 tcgacaaagc gcgtaatctg ctgtttgcca cggtagagat tgaatctgaa gaacggtgga
    26701 atgcggtagc cagcactgaa atctgccaac gttggtggaa atatatgacc gatgttatgc
    26761 ccgctaaccc ggataacagc ccggtgagta gcgagctgca agaagtgttt tacctgcctt
    26821 aatgagttgc ggtgccggaa aatatccagc accgcgactt catcagaaga agtatttaaa
    26881 gcgaacgcgt gcgccgtaac ggtcatcagt atcatcaccg tgttttttat cgccgtcgct
    26941 atccagaatg gagtaatacg tcccgaggta aatattaaag ttctccatat ccatcacgtt
    27001 agcgaactga taagacgcat gaatggtgtg aatggtgtac gtcccttcat catcaatcca
    27061 gcagtcgtta tcacaaacta caccgctgaa ttcatcaatc ttattgtgcg cgtagatata
    27121 accgagctcg aaacgtttcc acagcgcgtt aatcccggca gtgaaatctt tctcattatt
    27181 agcatctaaa taggcggtat ttagattaac cacaatgcca ttgtccggat ccgttttcag
    27241 gccgttccag gtcatgctca taccatagcc ggtacgatcg gactgatcga caaaattacc
    27301 cttgctatcg gtataaccat aagcattatt taccacgttc gcttccatcg ctgcggaaac
    27361 ggtgaattct tccgtcggcg accaggcaat tactgggcgc agataagcaa catttttctg
    27421 ctgctccata tcgcgtccat gataattacc gtcattatat aaagatgttc cgtcttcaag
    27481 taacgtgttt aattcaaaat accagttatc gagttgtttg ctgacgagga aattaccgcc
    27541 agcgttagag cgcccgcggc cctctttcat catatagata taaccgctgc catcgtcata
    27601 aagatcgttc gcagtattac cggaatgttc aacaaaggta tcctgattca atgggaacat
    27661 atcgtaggct tcaaaacggc ctactttgac cttccagtcg ttttctttgc caaagaagaa
    27721 taccgcatca tcgatattta ctgaaccgtg catatcaccc agcggttgtg cggagaaccc
    27781 ggcgaaataa ccgttgtcca tctttcgcat accgtcaaaa cctaacagaa tacgaccatt
    27841 gagattccac tgttcattag tgggatcgct attcacatca gcgttggtca ttgccagcag
    27901 gccatgatta ctttccgcat ccatattaaa ttctacgtcg ccgtagattt ttaaatcgcc
    27961 ataacctgaa agcaaaagat taggtgaggt ggcatttttc tttgcagatt ccgccgttgc
    28021 ctgtccattt accggcgtat tgccctgagc ggccttaatt tcgcggatct cagcggcttg
    28081 ttgtgtctgc agctgtttaa cctggctctc tgcttttgcc gcccgcattt ctgccgcttc
    28141 caggcgttct tccagcaatg ccatgcgttg ttccagcatt aaatgctgtg tttttgccat
    28201 accggtacta catgccaata ataaaccgat ggctaaagat aatgtcgatt ttttcattat
    28261 ttacgttccc tgaaataaag gagaccctac gctttccaaa aggaatacgt atatagagaa
    28321 atataaggtg tgcactttcg aatttttaat ccaacttcag caggccgatg gatagtatcc
    28381 cgccgcataa tatgagtaaa tc
//
LOCUS       DBFIFS010000040        27799 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.040, whole genome shotgun sequence.
ACCESSION   DBFIFS010000040 DBFIFS010000000
VERSION     DBFIFS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.040"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            48..710
                     /gene="acrS"
                     /locus_tag="N7A07_001619"
     CDS             48..710
                     /gene="acrS"
                     /locus_tag="N7A07_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU2476300.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(713..892)
                     /locus_tag="N7A07_001620"
     CDS             complement(713..892)
                     /locus_tag="N7A07_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU2476301.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(976..1860)
                     /gene="yhdJ"
                     /locus_tag="N7A07_001621"
     CDS             complement(976..1860)
                     /gene="yhdJ"
                     /locus_tag="N7A07_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU2476302.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(1945..2241)
                     /gene="fis"
                     /locus_tag="N7A07_001622"
     CDS             complement(1945..2241)
                     /gene="fis"
                     /locus_tag="N7A07_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU2476303.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2267..3232)
                     /gene="dusB"
                     /locus_tag="N7A07_001623"
     CDS             complement(2267..3232)
                     /gene="dusB"
                     /locus_tag="N7A07_001623"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU2476304.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3561..4442)
                     /gene="prmA"
                     /locus_tag="N7A07_001624"
     CDS             complement(3561..4442)
                     /gene="prmA"
                     /locus_tag="N7A07_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU2476305.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4454..5905)
                     /gene="panF"
                     /locus_tag="N7A07_001625"
     CDS             complement(4454..5905)
                     /gene="panF"
                     /locus_tag="N7A07_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU2476306.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5895..6137)
                     /locus_tag="N7A07_001626"
     CDS             complement(5895..6137)
                     /locus_tag="N7A07_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU2476307.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6246..7595)
                     /gene="accC"
                     /locus_tag="N7A07_001627"
     CDS             complement(6246..7595)
                     /gene="accC"
                     /locus_tag="N7A07_001627"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU2476308.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7606..8076)
                     /gene="accB"
                     /locus_tag="N7A07_001628"
     CDS             complement(7606..8076)
                     /gene="accB"
                     /locus_tag="N7A07_001628"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU2476309.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9054..10028)
                     /locus_tag="N7A07_001629"
     CDS             complement(9054..10028)
                     /locus_tag="N7A07_001629"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU2476310.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10180..12120
                     /gene="csrD"
                     /locus_tag="N7A07_001630"
     CDS             10180..12120
                     /gene="csrD"
                     /locus_tag="N7A07_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU2476311.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12425..13468
                     /gene="mreB"
                     /locus_tag="N7A07_001631"
     CDS             12425..13468
                     /gene="mreB"
                     /locus_tag="N7A07_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU2476312.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13534..14637
                     /gene="mreC"
                     /locus_tag="N7A07_001632"
     CDS             13534..14637
                     /gene="mreC"
                     /locus_tag="N7A07_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU2476313.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14637..15125
                     /gene="mreD"
                     /locus_tag="N7A07_001633"
     CDS             14637..15125
                     /gene="mreD"
                     /locus_tag="N7A07_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU2476314.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15134..15727
                     /gene="yhdE"
                     /locus_tag="N7A07_001634"
     CDS             15134..15727
                     /gene="yhdE"
                     /locus_tag="N7A07_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU2476315.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVVQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15717..17186
                     /gene="rng"
                     /locus_tag="N7A07_001635"
     CDS             15717..17186
                     /gene="rng"
                     /locus_tag="N7A07_001635"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU2476316.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17254..21054
                     /gene="yhdP"
                     /locus_tag="N7A07_001636"
     CDS             17254..21054
                     /gene="yhdP"
                     /locus_tag="N7A07_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU2476317.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21210..22655
                     /gene="tldD"
                     /locus_tag="N7A07_001637"
     CDS             21210..22655
                     /gene="tldD"
                     /locus_tag="N7A07_001637"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU2476318.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22789..23718)
                     /gene="aaeR"
                     /locus_tag="N7A07_001638"
     CDS             complement(22789..23718)
                     /gene="aaeR"
                     /locus_tag="N7A07_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU2476319.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23901..24104
                     /gene="aaeX"
                     /locus_tag="N7A07_001639"
     CDS             23901..24104
                     /gene="aaeX"
                     /locus_tag="N7A07_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU2476320.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24112..25041
                     /gene="aaeA"
                     /locus_tag="N7A07_001640"
     CDS             24112..25041
                     /gene="aaeA"
                     /locus_tag="N7A07_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU2476321.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25047..27014
                     /gene="aaeB"
                     /locus_tag="N7A07_001641"
     CDS             25047..27014
                     /gene="aaeB"
                     /locus_tag="N7A07_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU2476322.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27106..27378
                     /locus_tag="N7A07_001642"
     CDS             27106..27378
                     /locus_tag="N7A07_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU2476323.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27434..27697)
                     /gene="yhcN"
                     /locus_tag="N7A07_001643"
     CDS             complement(27434..27697)
                     /gene="yhcN"
                     /locus_tag="N7A07_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU2476324.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6667 a         6953 c         7410 g         6769 t
ORIGIN      
        1 attttctcac tctgtgtcga agatatttat ttcctgaata attaatcatg gcaaaaagaa
       61 ccaaagccga agctctgaag acccggcaag aactgattga aactgccatc gcccagtttg
      121 cgcaacatgg cgtaagcaag acgacgctca acgacattgc cgatgccgct aacgttacgc
      181 gtggcgctat ctactggcac ttcgagaaca agacccaact gtttaatgag atgtggttgc
      241 aacagccttc attgcgggag ttaatccagg aacacttgac ggctggatta gagcatgacc
      301 cgtttcaaca attgcgtgaa aaattgattg tcggcttgca atatattgcc aaaattcccc
      361 gccagcaggc gttgctgaaa atcttatatc acaaatgtga atttaatgat gagatgctgg
      421 ccgagggagt gatacgcgaa aagatgggct ttaatccgca gactctccgc gaagtattgc
      481 aggcgtgtca gcaacaaggt tgtgtagcaa ataacctcga tttagatgtt gtgatgatta
      541 ttattgatgg tgccttcagc ggaattgttc aaaactggtt aatgaatatg gcgggttatg
      601 atctttataa acaagccccc gctctggtcg ataacgtatt aagaatgttc atgccagatg
      661 aaaacataac gaaattaatt catcaaacga atgaattaag tgtcatgtaa attcacattc
      721 tgtcctgaaa attcagttcg tcacagttaa ccagttttag tggattaact gagttcatat
      781 tgcggcattt gtctgtttct gttgccagca gttcaatcca ctgcatcagt aaagtatcaa
      841 acagaaaaac gaagacagcg aaaacgacga gccaccaata cttgcgaatc attacctgag
      901 cctgtgcatg tttaaccaag tctggcagga aatatacacg aaatgaataa tctgcaaacc
      961 aggcttacac atcctttact ttgtaatgag atcggggtca acttcgctta accggcttcg
     1021 ttttgacagg ttgccggtct ttcttttttt cactttcgcc agttcttccg cagagtaatg
     1081 cgacgcgaca tccagccgtc gaagccccat tttgatgtac tcgctgttga tctcaatacc
     1141 aatgaatttt cgtccgctgg cgatggctac ggcaccggta gtaaagctac cagcaaacgg
     1201 gtcgagaacg atatcgcctg ggttggaaga ggcgagaata atgcgtttca gtaaggcttc
     1261 cggtttttgc gtcgggtggt tttcatattc atccatcaaa taacgcacgc gcggaaaatc
     1321 ccagacgtta cccggtactt tttgatgatt gtaaggctgt ggaggatttt tgcgataatc
     1381 gatcaacgcg cgctgcgatc cggttttggc ttctaccaga atagcatcac cgttgaatgt
     1441 gtagttcttt gcgtctttca ccatcatcag gatgggttcg tacatggagc cgtagtgttt
     1501 tttcgcctgc actccagaac tgtcatatga ccagacgatg cgacttttga tggtaaaaag
     1561 cttgcggcac tggagatcga taaagggcat gttttccgta ctgttcatga tgtacatgct
     1621 gccctgcttt ttcagaacgc ggtggcactc tgcaatcact tctaacagcc agtcgataaa
     1681 cagatcttct ttccaggctt cgatcagacc atcaaaattt ttaccgatgt tatacggtgg
     1741 gtcggcaaag atcagatcga cgctttcagt aggtagcttt ttaagttcgg caagggcatc
     1801 accgtgaata atggtcttag cttcattacc aaaccgggtc ggttcacatc ctgttctcat
     1861 ggtcactccc tttgtgacac ctataaaaaa ggcgcttccc catgccgagt agcgcctttt
     1921 aatcaagcaa ttagctaacc tgaattagtt catgccgtat tttttcaatt ttttacgcag
     1981 cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc tggttaccac gggtgtattg
     2041 catcaccatg tccaacaggg gctgttctac ttcagccagt accagctcat agaggtcatt
     2101 cacatcctga ccattcagtt gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg
     2161 caggggtttt tgggttacct gatcctgaga gttaacggta gaaacggtca gtacgtcaga
     2221 atttacgcgt tgttcgaaca tagttctgtc agctctttat ttctgtttac gcaaaatttt
     2281 cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc ctcaatggcg ttgaatgtgc
     2341 gccgaaactg gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa
     2401 ttcggtaccc ttttgccgga ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca
     2461 agcgcttaac ctctgccaaa ggcagcgggg gcagcaactc cccagtgtcc agataatgct
     2521 ggatttcccg aaagatccag ggtcttccct gagctgcgcg gcctatcatc agggcatccg
     2581 cccctgtata gtcgagcaca gctctggctt taagcgggtc agtaatgtcg ccattcgcga
     2641 taaccggaat ggaaactttc tgcttaactg cccgaatact gtcgtactca gcttctccat
     2701 tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca cagtcttcag
     2761 ccagttgggc aatctcttcg cagttacggt gttccggtgc ccagccggtg cgaatcttca
     2821 gggtaacagg aacgtccact gcattgacga cctcggtaag gatcgattta acgacatccg
     2881 ggtactgcaa gagggctgaa cctgcgagct tgcgattcac ttttttagcc gggcaaccca
     2941 tattgatatc aataatctgg gcaccgcttt ccacgttaat acgtgctgca tctgccattt
     3001 ctttcggatc gctaccagca atttgcacgg tgcgaatacc gggttcatca atgtgcacca
     3061 tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt agaagacatc atctcggata
     3121 ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag
     3181 ccatgggcgc tgcgatcagg cgatttctga gctgatattg tccgatgcgc atgagttaag
     3241 aaatgaccat actgtgactg caaggcggcg tatattacgc attttttgca cgagatgaaa
     3301 ggccaaactt tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg
     3361 tgcgataatt actcataaaa atcatcatat tagaaaatag tgactaaaat ttacactcaa
     3421 agaaatttga gtaagttctc aatttttctt tatgaatgaa aattttggca cgcaaatttg
     3481 cgtaaataat cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc
     3541 accctatggc gatgcgaagg ttaattctta cgaccggtaa tacggcacca ctcttctttt
     3601 tccacgaccg ggtccagagc gaagctgtcg gcataggctt cgcaaacgct ctccgcctgg
     3661 cttgccagaa taccagaaag gcccagtaag ccgcctgaaa ctggcaggac gctaattaac
     3721 ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga ccaccacgtc ggctttcatt
     3781 tcttctggct gatctttcgg taagtagagt tccagacggt cagaaacgcc attacgttcg
     3841 gcgttatcgc ggctggcctg aatcgcctgc ggatcgatat caataccaat ggcttttgct
     3901 gcacccagtt tcagcgccgc gatcgccaga atgccggaac cacagccaaa gtcgatgact
     3961 gttttaccgg ttaaatcgag gctgtcgagc cattgcaggc acagagaggt ggttggatgg
     4021 gtacccgtac caaacgccag ccctggatct aacatcacgt tgacggcgtt ttcgtccggc
     4081 acatcacgcc agctagggca gatccacagt cgttcgccaa agcgcatcgg gtggaaatta
     4141 tccatccatt cgcgctccca gtctttatct tctagttgtt cgattttatg cgcgaagcct
     4201 gcgccgagca gcggatggtt ttccagaatc gccaccacgt cgttcatatc ggtttcagcg
     4261 tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca
     4321 aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc
     4381 gcatcgctaa gatcttccgc gttcgcgccg gtggtgttca gtttcagttg gatccaaggc
     4441 atggcaaaac tctttattta tcagtagtca aaacggtagc ttgcgggacg gatgtaccga
     4501 aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg atagggtgga
     4561 agcccaggta ctgaatattc agcgtcgcga gtacggcata cagcacgccg ccaacgatca
     4621 tcgcacttag cgcgcctttg gcgttggcgc gttcccagta aagacccagc accagcggcc
     4681 acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt cagccagatg atcatttctg
     4741 gcggcttcca ggcggcaagc agcagcaacg cgccgagaac taacgtaatt accgccgaca
     4801 tccgcttcag acgcgtctcg ttttgcattt gatccggacg gatattcaga tagagatctt
     4861 taatgatcgt agcggaactt tgtagcaatt gggcgttaat tgtcgacatg atcgcagcca
     4921 taggtgcagc cagaaagatc ccggcagcaa acggtggcag cacttttacc attaacgtcg
     4981 ggatcaccag atccggtacg gtgagatcgg ggatcactgc acgacctaac gctccggcta
     5041 ggtgcatacc gaacatcagg attgcgacta caatcgtacc gatgatgatc ccccggtgta
     5101 ctgctttgct gtctttataa gagatacagc gcaccgcagt atgcggcagg cctataacgc
     5161 caaagcacac cagtacccag aacgacgtca taaaggcagg cgacagaata tcgtcagcgc
     5221 cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt ctgtactgcg ttgcttaagc
     5281 cgccagcggc atgtaccacg ccaataagca gcacaacggt gccaatcagc atcacaagcc
     5341 cttgcatggt gtcgttcagc acgctggcgc gaaagccacc aaaggcggta tataacgcaa
     5401 tgctgatacc aaaaatcagc agcccggttt cataaggaat acccgccgcg gtttccagca
     5461 ggcgcgcacc gccgataaac tgcacggtca tcgcaccaac gaacgcaacc agcaaactca
     5521 aactcgccag ccacaccaga agacgactct ggtagcgggc aaacagcata tcgttcagcg
     5581 tcactgcatt gtagcggcgc gcaagaatcg caaacttctt gccgagaata ccgagtgaaa
     5641 gccagactgc cggaagctga atcatcgcca gcaataccca gccaaggccg tatttgtaag
     5701 cagctccagg cccgccgata aacgaactgg cactgatata agtcgcggtg agcgtcatcg
     5761 ccagcacaat accgcccata gagcggctgc cgaggaaata ctcattaagg aaggtgccgg
     5821 tgctccgttt acgcatcgca taaaccgaga taccgaacac caccaccaga taggcgacca
     5881 gcggtagaat tacttcaagc tgcatcgtca tcctccagcg ggatatcgcg atagataaat
     5941 ttcaccatcg cccagcacag tccaataaac agcagcggcg tcaggatgca ggccatctca
     6001 aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag gtaagtaagc ggctactaac
     6061 caaactgcca gatacaaaag ggtcagcccc agcgcccagc gcgcctcttt atgggcctga
     6121 acaaaacgag tgtccatttt ttgtccctga tgggtgaaga aagcgaggat tgtaccttat
     6181 gggtgttgtc gatccccagt aataaaaaag gccggaaaat ccggcctttt gacgcttttg
     6241 cagtcttatt tttcctgaag accgagtttt ttctccagat agtggatgtt agtgccacca
     6301 tgctggaagt tctcgtcatt catgatgcgg atctgcagat caacgttggt tttgataccg
     6361 tcgatgatca gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc acggttttca
     6421 ccgtagcaaa tcagcttacc gatcattgag tcatagtacg gcggtacggt gtagcccgcg
     6481 tagatatgag actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg
     6541 cccggacttg gcaggaaggt gttcggatct tcggcgttga tacgacattc caccgcatgg
     6601 ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt gaccggcagc gatacgcagc
     6661 tgttctttga tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc tacctgaata
     6721 cgggtgttca tttcgatgaa atagaactcg ccgttttcga acaggaactc gaaagtacct
     6781 gcaccgcgat agccgatatc aacacacgct ttagcgcaac gttcgccgat gtagcgacgc
     6841 agttccgggg taatgcccgg tgctggtgct tcttcgacca ctttctggtg gcggcgctgc
     6901 atggagcagt cacgttccgc cagatagata gagttgccct gaccgtcagc cagtacctga
     6961 atctcgacgt ggcgaggatt ttccaggtat ttctccatgt aaaccatatc gttgctgaaa
     7021 gcagctttcg cttccgcacg ggtcatggag atggattgtg ccagttcagc gtcgccgcgc
     7081 actacgcgca taccgcgacc accgccgccg ccggaggctt tgataatcac cggataacca
     7141 atgcgtttag caatggcacg gtttttatcc atatcgtcgc ccagcgggcc gtcagaaccc
     7201 ggtacgcaag ggacgcccgc ttttttcatc gccgcgattg cggatacttt gtcgcccatc
     7261 aggcgaatgg tttctgcttt cgggccaatg aagataaagc cggagcgttc aacctgctcg
     7321 gcaaagttgg cgttctcgga gaggaagccg taacccggat ggattgctac tgcgccggtg
     7381 atttcagcgg cgctgatgat tgccgggatg ttcagataac tttttactga cggagcaggg
     7441 ccaatacaga ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc cgcgctggag
     7501 tgcacagcga cagtcttgat gcccagttct ttacaggcac gaagaatacg caatgcaatc
     7561 tcgccgcggt tggcaataac aattttatcc agcatgttcg cctcgttact cgatgacgac
     7621 cagcggctcg tcaaattcta ccggttgtcc actttcgacc agaattgctt tcacggtacc
     7681 ggatttgtcc gcttcgatct ggttcatcat tttcatggct tcaacgatgc acagggtatc
     7741 gcccacgttg actttctgac ccacttcgat gaacgctttt gcatccgggc ttggggtgcg
     7801 gtagaaagta ccaaccatcg gggaacgtac gatgtgacca ctgatttccg ctgctgctgg
     7861 cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta gattgagctg gctgctgcat
     7921 cattggtgca gcgtaagctt gttgcatcac agggaaactt gcggcaggag ctgcacggct
     7981 aatgcgtact gactcttcgc cttcagaaat ttccagttcg gagatgcctg attcttcaac
     8041 cagctcgatc agttttttaa tcttacgaat atccatgagt gggttccgta ctctttgttt
     8101 agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg acagctgtat
     8161 gcatagcgat aaattccagc aggtcgggac gccggtctat tttgccccag agtgtcaatg
     8221 ttagacttga cggacattgt gcagcgatct gctgctatcc tccggcaaaa aacaaaatat
     8281 accttcgttg cgccaatgtc accttttccg cagcgcaaaa actgcgtcag ggaggacgag
     8341 gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa tactggtctt atcagggaag
     8401 ataatcaaca actaacatgt aaataacctt caacaccgtg taatttgcaa caagccgcac
     8461 aattcacgaa attagcgcca tcatcgacgg aacttcttat aacgtaaggt gaaaagctgt
     8521 aaagccagcc ctgcgaagat gaggggctgc aatgagataa tcgtcacaga ccacaaataa
     8581 tgtagggatg ccaggatcgc gacaagatag acgacattac gcagcggttg ctggggatta
     8641 cccaattttc actgcatcgc ctggatagat gtgaatgcta aagcacgcaa aattaaccga
     8701 ttgacaatgc tcagcattaa ataggttcga gtgattaatt tgtagtcaaa caaaatcaga
     8761 ttattcacct catggtacca gaaataagta aggggttaag cgtagcgtcg cccaggcaaa
     8821 gcaccataaa cctaacaggc gacggaggcg tatcaataat agctgtttag tggagtatca
     8881 atgcgcatca atttatggtc agtcgaaatc atgactttcc agtttggatg atgttgtaca
     8941 acttcatgac caaggtgttc gacgaaagct gtccggctaa ccgcttttcc cgttaaccga
     9001 ttgaataatg atttcaaaat gagttccctc tcttattatt ccttgctaaa tggttagtta
     9061 accttcacca gcgtgcgacc ctggatctgg ttattaatga tggcctcggc aaagttcggt
     9121 gcctctgaca gagatatctc ttttgccgcc tgggtataga atgattccgg taaatcggcg
     9181 accagtcgct gccaggcttg tgcgcggcgt tctggtggcg tcattactga atccacccct
     9241 tgcaaacgga cattacgcag aataaatggc atgaccgtgg ttggcagagt aaaaccaccc
     9301 gccagaccac aggccgccac gcagccgccg taattcattt gcgccagcac ttttgccagc
     9361 actttgtcgc caacagtgtc aattgcccca gcccagactt gtttttccag aggacgggat
     9421 tcggcaaact catcacgagg gagaatacgg ctggcaccta aacttttcag atattcatgg
     9481 gtactttcgc gaccggaaac ggcaacgacc tgataaccca acttatgcag cagcgccacg
     9541 gcggtactgc cgacgccacc actggcaccc gtcacgacaa tctccccgtc ctgcgggcga
     9601 acaccggcat cttccagcgc catcacacac agcatggcgg taaaaccggc agtaccgata
     9661 atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc acctttcact
     9721 cgcgcctgct ccgccagccc accccagtgg ttttcaccaa cgccccagcc agtgagtaac
     9781 acctcctgac cggcatgaaa acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg
     9841 atcccaggaa tcatcggaaa attacggatg atttttccct taccggtaat cgccagcgca
     9901 tctttatagt tcaggctcga ccagtgaaca tcgaccgtga catcgccctc cggcaggcga
     9961 ctttcgtcca gagtctgtac tgatgcgaga gttttgccgt cctgctgttc taaaagtaac
    10021 gcctgcataa gtggtcctca tgtgcatgat ggattggaaa ataatttctg aagactatac
    10081 tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa tctgatttgc
    10141 tagtatgccc gcttcctcac tatcggagtt aacacaagga tgagattaac gacgaaattt
    10201 tcggcctttg ttacgctgct caccgggtta acaatttttg tgactttgct gggctgttcg
    10261 ctaagtttct acaacgccat tcagtataag tttagtcatc gcgttcaggc ggtggcgacg
    10321 gcgattgata cccaccttgt gtcgaatgac ttcagcgcat taaggccaca aattaccgaa
    10381 ttaatgatgt cggcagatat cgttcgtgta gacctgctcc atggtgataa gcaggtttat
    10441 accctggcca gaaatggtag ttatcgtccg gttggcacca gcgatctgtt tcgtgaactg
    10501 agcgttccgt tgataaagca tccggggatg tcgttgcgtc tggtttatca ggatccgatg
    10561 ggcaactatt tccattcgtt gatgaccacc gcgccgctca caggggcgat tggctttatc
    10621 attcttatgc tctttctggc ggtacgctgg ttacaacggc aacttgccgg gcaagaattg
    10681 ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc
    10741 atctatgaat ggccgcccag aaccagcagt gcgctggata cgctgcttcg tgaaattcag
    10801 aacgcacgcg aacaacacag ccgtcttgat acgctgatcc gctcttatgc cgcacaggac
    10861 gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc agttagcaac gttactggaa
    10921 gatcaggaga aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat
    10981 atgttgagcg atacctgggg gcacagccag gttgaagaac agttcttcac tctgacgaat
    11041 ctgctgtcga catttatgat gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt
    11101 gattttgctg cgctgttacc gcaccggacg ttaaaagagg cagagagcat cgccggtcag
    11161 ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga cgatatgatc
    11221 cacattggta tctgcgcctg gcgtagtggt caggataccg agcaggtaat ggaacatgca
    11281 gagtctgcca cgcgtaatgc gggattgcag ggcggcaata gctgggctat ttacgatgac
    11341 tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc
    11401 agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat
    11461 catcgcgaac tcatgtgccg catctttgat ggtaatgaag aggttagctc ggcggagtat
    11521 atgccgatgg tcttgcagtt tggcttatcg gaagagtatg accgtctgca aatcagccgt
    11581 cttattccac tattgcgtta ctggccagag gaaaatctgg cgattcaggt taccgttgag
    11641 tcgctgattc gcccgcgttt tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa
    11701 tcacaacgaa aacgcataat tattgaactt gcagaggccg atgtaggtca acatatcagt
    11761 cgtttacaac ctgttattcg tttagtgaat gctttagggg tacgggtagc cgtcaaccag
    11821 gctggtttga cgctggtaag caccagttgg atcaaagaac ttaatgttga gttactcaag
    11881 ctccatccgg ggctggtcag aaacattgag aagcgaacgg agaaccagct gctggttcaa
    11941 agcctggtgg aagcctgctc agggaccagc acccaggttt acgccaccgg tgtgcgttcg
    12001 cgaagcgagt ggcagaccct gattcagcgc ggtgttacag gcgggcaagg ggattttttc
    12061 gcgtcctcac agccacttga tactaacgtg aaaaaatatt cacaaagata ctcggtttaa
    12121 cctgccgttt aatccgtttt cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct
    12181 tgtctgctcg ccagattgtt gcagcacata tgcagatgaa tgaccttacg cggttgcaaa
    12241 caggcgagga atgctgctga tgcattaagc ctttctggac tcaggcagag atttgtaaca
    12301 aaggaaacga actgcactaa ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct
    12361 gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc cagctttcag gattatccct
    12421 tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg acctgggtac
    12481 tgcgaatacc ctcatttatg taaaaggaca aggcatcgta ttgaatgagc cttccgtggt
    12541 ggccattcgt caggatcgtg ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc
    12601 gaagcagatg ctgggccgta cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg
    12661 cgttatcgcc gacttcttcg tgactgaaaa aatgctccag cacttcatca aacaagtgca
    12721 cagcaacagc tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac
    12781 ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt
    12841 cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac
    12901 cggttctatg gtggttgata tcggtggtgg taccactgaa gttgctgtta tctccttgaa
    12961 cggcgtggtt tactcctctt ccgtacgcat tggtggtgac cgtttcgacg aagctatcat
    13021 caactatgtg cgtcgtaatt acggttctct gatcggtgaa gccaccgcag aacgtatcaa
    13081 gcacgaaatc ggttcggctt atccgggcga tgaagtccgt gaaatcgaag ttcgtggccg
    13141 taacctggca gaaggtgttc cacgcggttt taccctgaac tccaacgaaa tcctcgaagc
    13201 actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg aacagtgccc
    13261 gccggaactg gcttccgaca tctccgagcg cggcatggta ctcaccggtg gtggcgcact
    13321 gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc attccagtcg ttgttgctga
    13381 agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca
    13441 cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc aggggaagcg tctgtttacc
    13501 ctgcctggtc tgatacgaga atacgcataa cttatgaagc caatttttag ccgtggcccg
    13561 tcgctacaga ttcgccttat tctggcggtg ctggtggcgc tcggcattat tattgccgac
    13621 agccgcctgg ggacgttcag tcaaatccgt acttatatgg ataccgccgt cagtcctttc
    13681 tactttgttt ccaatgctcc tcgtgaattg ctggatggcg tatcgcagac gctggcctcg
    13741 cgtgaccaac tagaacttga aaatcgggcg ttacgtcagg aactgttgct gaaaaacagt
    13801 gaactgctga tgcttggaca atacaaacag gagaacgcgc gtctgcgcga gctgctgggt
    13861 tccccgctgc gtcaggatga gcagaaaatg gtgactcagg ttatctccac ggttaacgat
    13921 ccttatagcg atcaagttgt tatcgataaa ggtagcgtta atggcgttta tgaaggccag
    13981 ccggtcatca gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa actgaccagt
    14041 cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat
    14101 atccgcgtaa ttgcagccgg taacggttgt acggatgatt tgcagcttga gcatctgcca
    14161 gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc
    14221 ccggaaggct atccggtcgc ggttgtctct tccgtaaaac tcgataccca gcgcgcttat
    14281 actgtgattc aggcgcgtcc gactgcaggg ctgcaacgtt tgcgttatct gctgctgctg
    14341 tggggggcag atcgtaacgg cgctaacccg atgacgccgg aagaggtgca tcgtgttgct
    14401 aatgaacgtc tgatgcagat gatgccgcag gtattgcctt cgccagacgc gatggggcca
    14461 aagttacctg aaccggcaac ggggatcgct cagccgactc cgcagcaacc cacgacagga
    14521 aatgcagcta ctgcgcctgc tgcgcccaca cagcccgctg ctaatcgctc tctacaaagg
    14581 gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg
    14641 cgagctatcg tagccaggga cgctgggtaa tctggctctc tttcctcatt gcgcttttgc
    14701 tgcaaatcat gccctggccg gataacctga ttgttttccg gccaaactgg gtgttactca
    14761 tcttgttgta ttggatcctg gccttgcccc atcgcgtaaa tgtgggcaca ggttttgtga
    14821 tgggtgccat actggatctg atcagcggct cgacgcttgg cgtacgcgta ttggcgatga
    14881 gcatcattgc ttacctggtg gcgctgaaat accagctttt ccgcaacctc gcattatggc
    14941 agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt ttctgggcag
    15001 agtttttagt gattaacgtc tctttcagac cggaagtgtt ctggagtagt gtagtcaatg
    15061 gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag tttgcagtgc
    15121 aataaaggtt tctatgactt ctctgtattt agcttccggt tctccgcgtc gtcaggagtt
    15181 acttgcgcaa cttggcgtga cctttgaacg tattgttacg ggcattgagg agcagcgtca
    15241 gccgcaggag agcgcgcagc agtatgttgt gcgtctggcg cgcgagaaag cacgggcagg
    15301 tgtcgtgcaa acggcgaagg atctcccggt gctgggtgcg gatactatcg ttatcctgaa
    15361 cggagaagtg ctggagaaac cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt
    15421 atcgggtcag acccatcagg tgatgacggc agtggcgttg gccgacagcc ggcacattct
    15481 cgattgcctg gtggtcaccg atgtgacttt cagaacgtta acagacgaag acatcgcggg
    15541 ctatgtcgcc agcggtgaac cgttagataa agcaggtgca tacggtattc aggggctggg
    15601 tggctgtttt gtcaggaaga taaatggcag ctatcaagcc gtagtcggct taccgctggt
    15661 tgaaacgtat gaattattaa gtaattttaa cgcactgcgt gagaaaaggg ataaacatga
    15721 cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat attgatggcg
    15781 gtattctgca ggaaattcat attgaacgtg aggcgcgacg cggaatagta ggcaatatct
    15841 acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta gatattgggc
    15901 tggataaagc cgcgtttctt catgcatctg acatcatgcc gcacaccgaa tgtgtggcgg
    15961 gtgaagaaca aaagcaattc acggtgcgcg acatctcgga actggttcgt caggggcaag
    16021 atctgatggt gcaggtggtg aaagatccgc ttggcactaa gggggcgcgc ctgaccaccg
    16081 atatcacgct gccttctcgc tatctggtct ttatgccagg ggcttctcac gttggggttt
    16141 cccaacgaat tgaaagcgaa tcagaacgtg aacgcctgaa aaaagtggtc gcagagtatt
    16201 gcgacgagca gggcgggttt atcatccgta ccgcagcgga aggggttggc gaggctgaac
    16261 tggcctccga tgccgcttat ctgaaacgcg tctggaccaa agttatggag cgtaaaaagc
    16321 gcccgcagac ccgttatcag ctgtacggcg aactggcgtt ggcgcagcgt gttctgcgtg
    16381 atttcgccga tgccgaactg gaccgcattc gcgttgactc acgcctgact tacgaagcgt
    16441 tacttgagtt cacctcggag tacattcccg agatgaccag caagctggag cattacaccg
    16501 gacgccagcc gattttcgat ctctttgatg tcgaaaacga aatccagcga gcgctggaac
    16561 gcaaagttga actgaaatcc ggtggttatc tgattatcga ccagaccgaa gcgatgacca
    16621 ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa tctggacgac accattttca
    16681 ataccaatat tgaagcgacg caggctatcg ctcgccagtt acggttgcgt aatctgggcg
    16741 ggattatcat tattgatttc atcgatatga ataatgaaga tcaccgccgc cgagtgctgc
    16801 actcgctgga gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg
    16861 cgctggggct ggtggagatg acgcgtaaac gcacccgcga aagcattgag cacgtactgt
    16921 gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct
    16981 atgaaatcat gcgcgagatt gttcgtgtac accatgctta cgactccgac cgtttcctgg
    17041 tctatgcttc tccggcagta gccgaagcct tgaaaggcga agagtcacac tcgctggcgg
    17101 aagtggaaat tttcgttggc aaacaggtta aagtacaaat tgaaccgctc tataaccagg
    17161 agcagtttga cgtcgtaatg atgtaaacag atgctgggcc gccatccggc aaagggtttt
    17221 tgagtcacat ttttagcaga caaggagtga cgggtgaggc gattgccggg gattttactg
    17281 cttactggag ccgcgctcgt tgtgatcgct gccctgctgg ttagcggcct gcgtattgct
    17341 ttaccgcatc ttgacgcctg gcgtccggaa atcctcaaca aaatagaatc cgcgactggc
    17401 atgccggtag aagccagtca gctctcagcc agctggcaga attttggccc gacgcttgaa
    17461 gcgcacgaca tccgtgcaga actaaaggat ggcggcgaat tttcggttaa acgcgttact
    17521 ctggcgctgg atgtctggca gagcctgtta catatgcgct ggcagtttcg cgacctcact
    17581 ttctggcagc tgcgctttcg caccaacact cctatcacca gcggtggtag cgatgacagt
    17641 ctggaagcca gtcacatcag cgatctgttt cttcgtcaat ttgaccattt cgatctccgc
    17701 gacagtgaag tcagtttcct gacgccatcc ggtcagcgcg ccgagctggc gatcccacaa
    17761 ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag cctctccagc
    17821 cttaccggac agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg
    17881 ttaagcaatg gtcgcgtctg gctccaggcg gatgacatcg acctgaagcc gtggctcggt
    17941 aaatggatgc aggacaatat tgcgctggaa acggcgcagt tctcccttga aggctggatg
    18001 acgatcgaca aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg tggtgccagc
    18061 tggttgggcg agaaggaaac gcatacgctg tcggtggata atctgaccgc gcatattacg
    18121 cgtgaaaatc cgggatggca gttctctatt cccgatacac ggatcacgat ggacggcaaa
    18181 ccctggccga gcggagcatt gacgctggcc tggataccgg aacaggacgt tggcggcaaa
    18241 gacaataaac gcagtgacga actccggatt cgcgccagta atctggagct ggcaggcctg
    18301 gagggcatac gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt ttggcgctct
    18361 acacaaccga gcggcaagat taacactctg gcgctggata tcccgcttca ggcggcagac
    18421 aagacccgtt ttcaggcatc gtggagcgat ctggcctgga agcaatggaa attattaccg
    18481 ggtgcggaac acttctccgg gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg
    18541 tcgatgaagc aggcaaagat gccttacgaa acggtattcc gtgcgccact ggaaatcgcc
    18601 gacggccagg caactataag ctggctgaac aatgacaaag gtttccagct ggatgggcgt
    18661 aatattgacg ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta cctgcaacct
    18721 gctaacgatg aaccctggct gggtattctg gctggcatca gtaccgatga tggttcacaa
    18781 gcctggcgct atttcccgga aaacttgatg ggtaaagacc tggttgatta cttaagtggc
    18841 gcgattcagg gcggtgaagc ggataacgcg acgctggttt atggtggcaa tccgcaactc
    18901 ttcccctata aacacaacga aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag
    18961 tttgccttcc agccggactg gcctgcatta actaaccttg gtattgaact ggactttatt
    19021 aacgacggtt tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat
    19081 ctcaccgcag tgatccctga ctactcaaaa gaaaaactgc tgattgacgc tgacattaaa
    19141 ggtccgggta aagccgttgg cccttacttt gatgagacac cgctgaaaga ttctctgggt
    19201 gcgaccctgc aagaactcca gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc
    19261 ccgctgaacg gcgagctggt aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg
    19321 tttatcaaac cactcgacag caccctgaaa aatttgagcg gtaaattcag ctttatcaat
    19381 ggcgatctgc aaagtgaacc actgacagca agctggttta atcagccgtt gaacgtggat
    19441 ttttccacca aagaaggggc aaaagcctac caggtcgcgg tgaatctcaa cggtaactgg
    19501 caaccggcga aaaccggcgt tctgcctgca gcggtgaacg aagcattgag tggcagcgtg
    19561 gcgtgggatg gtaaagtggg cattgttctg ccttatcatg ctggcgcgac gtataacgta
    19621 gagctaaacg gcgatttgaa gaatgtgagc agtcacttac cttcaccgtt agccaaacct
    19681 gcgggtgaac cactgccggt aaacgttaag gttgatggca atctcaacag ctttgaatta
    19741 accggacagg ctggtgcgga taatcatttc aatagccgct ggttgctcgg tcaaaagctg
    19801 acgctcgacc gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa
    19861 agtggtgttg aactcaatat gccgccgatg aatggtgccg agtggctggc cctgtttcag
    19921 aaaggtgcgg cggagagtgt cggtggtgca gcgagtttcc cacaacacat aacgttacgt
    19981 acgcctatgt tgtcgctggg aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg
    20041 acggcaaatg gcacccaggt tgaggcgcag gggcgtgaaa tcaacgccac gctggcgatg
    20101 cgtaataacg cgccgtggct ggcgaatatc aaatatcttt attacaaccc gagcgtggcg
    20161 aaaactcgtg gtgattcaac gccgtcatca cctttcccga caacggaacg cattaacttc
    20221 cgtggctggt cggacgccca aatacgatgc gcagagtgct ggttctgggg gcaaaaattc
    20281 gggcgcattg acagtgatat caccatttct ggcaatacat taacgctgac caatggactg
    20341 attgatactg gtttttcgcg gctcactgcc gatggtgaat ggattaacaa tccggggaat
    20401 gaacgtacct cgctgaaagg aaaactgcgc gggcagaaaa ttgatgccgc cgcagaattt
    20461 tttggtgtca cgacgcccat acggcagtcg tcatttaatg tggattacga tttacactgg
    20521 cgtaaagcac cgtggcagcc agatgaggcg acgttgaatg gcatcattca tactcaactg
    20581 ggtaaaggcg aaattaccga aatcaatacc ggacatgccg ggcaattgct gcgcttattg
    20641 agcgtagatg ccctgatgcg taagctgcgt tttgatttca gagacacttt tggcgaaggg
    20701 ttctattttg actccattcg cagcaccgcg tggattaaag acggcgttat gcacaccgac
    20761 gacacgctgg tggatggcct ggaggcggat atcgccatga aagggtcggt aaatctggta
    20821 cgtcgcgacc tgaatatgga agcggttgtc gcaccagaga tttctgcgac agtgggcgtg
    20881 gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag tgtttgccgc cagtaaagtg
    20941 ctggggccgc tgtggagcaa agtctccatt ttgcgctatc acatttcggg tccgctggac
    21001 gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac
    21061 gagggcgcgt aattgcccca atctcatagg ataatcgttg ccaaaggcca acgagccaga
    21121 acataaccgt aggctggata gggcgttcac gccgcatccg gcagccgtaa aaaatcctct
    21181 actgcagtaa ctaacgagta gcaaaaacga tgagtcttaa cctggtaagt gaacaattgc
    21241 tagcggcgaa cggcctgaaa catcaggact tgttcgtgat cctcggtcaa ctggccgaac
    21301 gtcgccttga ttatggcgat ctctattttc agtcgagcta tcacgaatcc tgggttttag
    21361 aagaccgcat tattaaagat ggttcttaca acatcgatca gggcgttggt gtgcgtgcaa
    21421 tcagcggtga aaaaaccgga tttgcttacg ctgaccaaat cagcctgctg gcgctggaac
    21481 agagtgcgca agcggcgcgc accatcgccc gtgatagcgg tgatggcaaa gtacagacgc
    21541 tgggcgcggt agagcatagc ccgttgtata cctcggtaga tccgctgcaa agcatgagcc
    21601 gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt tgcccgcgaa gcggacaagc
    21661 gcgtacagga agtgactgcc agcctcagtg gtgtctatga attaattctg gttgcggcca
    21721 ccgacggcac gctagcggcg gatgtccgtc cgctggtgcg tctttccgtg agcgttctga
    21781 tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg cggcggtcgt tttggttatg
    21841 agttcttcct tgccgatctc gacggcgaag tccgtgcgga tgcatgggca aaagaagcag
    21901 tacgtatggc gctggtcaat ctttctgccg ttgctgcacc agcgggcacc atgccggtag
    21961 tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc ggtaggtcac ggtctggaag
    22021 gcgacttcaa ccgccgtggc acttcagtat ttagtggaca ggtcggggag ctggtggctt
    22081 cagaactgtg taccgtggtt gatgacggta cgatggtcga tcgccgtggt tcggtggcga
    22141 ttgatgacga aggtacgcca ggccagtaca acgtgctgat tgagaacggc attctgaaag
    22201 gctacatgca ggataaactc aacgcgcgtt tgatggggat gacgccgact ggcaacggtc
    22261 gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac caacacctat atgctgccgg
    22321 gtaaatcgac cccgcaggaa attattgaat ccgttgagta cggtatctat gcaccgaact
    22381 ttggtggcgg tcaggtggat atcacctccg gcaaattcgt tttctccact tcagaagcat
    22441 atctgattga aaacggtaaa gtaacgaagc cggtgaaagg cgcaacgttg attggttccg
    22501 gtatcgaaac catgcagcag atttcgatgg ttggcaacga cctgaaactg gataacggcg
    22561 tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg cgtgggccag ccaacgctga
    22621 aagttgataa cctgactgtt ggcggtactg cgtaataatt tcagtatttt cagaaggata
    22681 attaatcctt cctcgtgcac gaacggtccc ctcgcccctt tggggagagg gttagggtga
    22741 ggggaacccg ttggcacagg tttgtacaac gtaacagtac aatatgaatt acttctcttt
    22801 cccgcgcccg tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga
    22861 gttgatcacg acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac
    22921 cgggcgtgga tctgactgat aacgcggcag caggatctcc agctccccac gattgatctc
    22981 gttgatcacc cacatcagcg gcacgtaggc gatcccggca cccgccgtta gccagcgcac
    23041 cagcgtcatc ggatcattag tcacaaatct cccttgcggg atcaggcgag tcgagatccc
    23101 ttccggtgca atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg
    23161 gctacttaaa tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc
    23221 gcacaccacc attggcatcg cgccaagacg gcgggaaaac aggctggaat cctgcaacgc
    23281 gccgacgcgg atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt
    23341 aaccagattg acgctcaaac ctgggtattc tttcagcatt ttggctgtca gcccggcgag
    23401 aacattttgt gccatagttg aagaacagcc aatgcgtagc gtcccgatgg gggtgttatt
    23461 gaaggcatac agttgctcat gaacatcctg cacttcatga agcatacgac ggcagccctg
    23521 gtagtaaatt ctaccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt
    23581 tacctgcaac tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg
    23641 tagctgtctg gcggcggcgg taaaagagcc aaattcaact actttggcaa acaccgacat
    23701 gcgttttagt cgttccatta ttcactctga cttaaaagtg atttagatca cgtaatatag
    23761 ataacagcat aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg
    23821 cccggctttc catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc
    23881 tctatacaac caaggtcaac atgagtctgt ttcccgttat cgtggtgttt gggctgtcct
    23941 tcccaccgat attttttgaa ttgcttttat cactggcgat tttctggctg gtgtgccggg
    24001 tacttgtgcc aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc
    24061 tctattgctg cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca
    24121 ctaataagaa aattctcccg tacggccatc acggtcgtat tagtcattct ggccttcatc
    24181 gcaattttta atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt
    24241 agcgctgacg tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt
    24301 cataaccagc tggtgaaaaa aggacaggtt ctgttcacca tcgaccagcc gcgctatcaa
    24361 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    24421 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    24481 gaccaggcca acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    24541 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gcgcgccagc agatggctgg
    24601 gtgaccaacc tcaacgtcta taccggtgag tttattactc gtggatcaac ggcggttgcg
    24661 ctggtgaaac agaactcttt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24721 gtgcgccctg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24781 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24841 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24901 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24961 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    25021 cgcctgcgtg agtttggtta atcacgatgg atattttctc cattgctaac caacatattc
    25081 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    25141 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    25201 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    25261 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    25321 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    25381 tggtacgaat agaaaactcg tatgcgtggg ggctggccgg ttataccgcg ctgatcattg
    25441 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    25501 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    25561 tcaaacaaga agtggatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    25621 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    25681 gacgcaccac ggcgctacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25741 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25801 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25861 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25921 gaagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25981 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    26041 ccaccgaaga agagatcctg caaggcgaac cggaagtcaa agtagagtca gccgaacgtc
    26101 atcatgcgat ggttaacttc tggcgaacca cactttcctg cattctggga acgcttttct
    26161 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    26221 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tacgggacgc
    26281 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    26341 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcgga atagaagtac
    26401 agaaacggcg actgggctcg atgggggcgc tggccagcac cataaatatt atcgtgctgg
    26461 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    26521 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    26581 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    26641 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    26701 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcccaccagc
    26761 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26821 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26881 gtcagttgct ggaagaactg gaaatctacc aggaaaagct atgcatctgg caagcgccac
    26941 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    27001 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    27061 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatacc
    27121 tttgattttg atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    27181 ggtctggcga aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    27241 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    27301 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    27361 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    27421 agacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    27481 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    27541 gcatatccat tggcgaagac gccacaccac ttacggatac ggtcccgatt gcttcacgat
    27601 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    27661 gtacgcttaa tgcagcaaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27721 ttagctgggc ctttgtttcg tgacccatat cacaaaatca agtatacact aattattgag
    27781 ctaattaata ccgcgctaa
//
LOCUS       DBFIFS010000041        27184 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.041, whole genome shotgun sequence.
ACCESSION   DBFIFS010000041 DBFIFS010000000
VERSION     DBFIFS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.041"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            80..1096
                     /locus_tag="N7A07_001644"
     CDS             80..1096
                     /locus_tag="N7A07_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU2476325.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1181..1603
                     /locus_tag="N7A07_001645"
     CDS             1181..1603
                     /locus_tag="N7A07_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2476326.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1600..1803
                     /locus_tag="N7A07_001646"
     CDS             1600..1803
                     /locus_tag="N7A07_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476327.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1807..2214)
                     /gene="cueR"
                     /locus_tag="N7A07_001647"
     CDS             complement(1807..2214)
                     /gene="cueR"
                     /locus_tag="N7A07_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU2476328.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2211..3386)
                     /locus_tag="N7A07_001648"
     CDS             complement(2211..3386)
                     /locus_tag="N7A07_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HGU2476329.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3383..5545)
                     /locus_tag="N7A07_001649"
     CDS             complement(3383..5545)
                     /locus_tag="N7A07_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGU2476330.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALLLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5549..21276)
                     /locus_tag="N7A07_001650"
                     /pseudo
     CDS             complement(5549..21276)
                     /locus_tag="N7A07_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(21313..25698)
                     /locus_tag="N7A07_001651"
     CDS             complement(21313..25698)
                     /locus_tag="N7A07_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU2476331.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMRDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25801..27156)
                     /locus_tag="N7A07_001652"
     CDS             complement(25801..27156)
                     /locus_tag="N7A07_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HGU2476332.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5060 a         8015 c         7882 g         6227 t
ORIGIN      
        1 cgcagtttat ttcttaaaac aatattattt gtttcttata gaaacattaa tacgacttat
       61 tttgaacaag agaaaatgaa tgaaaactgt aaacgtagct ttactggcac tcataatttc
      121 agcaacatcc agccctgttg ttttagctgg tgataccatt gaagcggcgg caacagagct
      181 ttcagccatt aactctggca tgtcgcaatc ggagattgag cagaagatta cccgcttttt
      241 agaacgcaca gacaacagcc ccgctgcgta tacctatttg actgaacatc actacatccc
      301 ttctgaaaca cctgatacca ctcagactcc cactgtccag acagatcctg acgcaggaca
      361 aaaaaccgtt gccgctacag gtgatgtaca gacaactgcc cgttatcaga gcatgatcaa
      421 cgcccgacag tctgcggtaa ctgacgccca gcaaacgcaa attacagagc aacaggcgca
      481 gatcgtagcc acacaaaaaa cgctcgccgc gactggagat acgcaaaata ccgcgcatta
      541 tcaggaaatg attaatgcca gactggcggc tcaaaatgag gctaatcagc gcaccgccac
      601 tgaacaaggg cagaaaatga atgcgctgac aaccgatgtg gcagtacaac agcaaaatga
      661 aaggactcaa tacgataaac aaatgcaaag tctggcgcag gagtctgccc aggcacatga
      721 acaaattgac agcctgtcac aagacgtaac ccaaacgcac caacagttaa ccaacaccca
      781 aaaacgggtt gcagataaca gccagcaaat taacacgctc aataaccatt tcagttcgct
      841 aaaaaacgaa gttgatgaca atcgtaaaga agccaatgcg ggaactgcat ctgccatcgc
      901 tatcgcctca caaccacagg ttaaaaccgg tgacgtgatg atggtgtcag cgggagcggg
      961 aaccttcaac ggtgaatctg cggtgtctgt cggaacatca tttaatgccg gaacgcatac
     1021 ggtacttaaa gccggtattt ctgcggatac acaatctgat ttcggcgcag gtgtcggcgt
     1081 gggatattcg ttctaatatt tcaatcctca atataaataa gagcaaggaa gcttgccggg
     1141 ttcacctctt cattaatttg tacattattt aaggttaaca atgatgaata gctccattaa
     1201 atcgttttcc ctgctggcgg ttatattact ggctggctgt agttcaccca cttcccgcat
     1261 cgcagattgc caggcgcagg gcgtcagtca tgacacctgt tacctcgcag aacagcagcg
     1321 tcaggcggct attttaagtg catccgaggc acaggcattt aaaaatgcag aagccgcaca
     1381 acacgcccag gcggcaaaga aagccattta taaaggattt ggcatgacct ttagaatgag
     1441 cagtaaaaac tttgcttatc tcaatgattc attatgtgca attgatgaag acaataaaga
     1501 tgccactgtt tatcagtcag gtctatataa cgtcattgtt tatcatcaca caggaaaagt
     1561 cgccttaatg aaagaaggcc agtttgtggg ttatttaaaa tgaaggagca aaggaaaata
     1621 cccctgacgc atattatgat tatcggtgcg tttatttttg ccttcttgca agtagtatta
     1681 ttagcctccc tggttcacgc tgtgaatgtt aacaacgaaa tccaggaagg cttatttcag
     1741 tcggggcgca ttatggtaga aagtttgcag catattcttt cggtgcaaac ggggattcac
     1801 tgattttcac cccgcccgat gatgacagca gccggagaga ttttcgataa tcgggcagtc
     1861 ggcgctgtca tcaccggggc aggcattcgc cagcgccagt agctggttgc gcatagattg
     1921 cagttcttcg atatgccgtt caatctccgc caccttctcc agcgtgcgac gtttgacgtc
     1981 ggcactgtga cgctgcgggt cgttaaacag attcaccagc tcgccgctct cttccaggtt
     2041 aaagcccacc tggcgcgcct ggcgcagtaa ggtcaattcg ttgagatgct gctgcgtgta
     2101 ggttcgataa ccattttcgc tgcgcatcgg cggcgtcacc agccccttct cttcatagaa
     2161 gcgaatggct ttgctggtca ggccggtaat ttttgctaca tcgctaatgt tcatcgttcg
     2221 cgcaacgcct cctgtgcttt gttaaagggt ttcaacagat aatccagcac gctttttttg
     2281 ccggtgcgga tatcgaccgt cgccaccata ccggggaata tcggaaactg ctgtttgctt
     2341 ttgttttcca gatggttgct gaaagtgcga atgtacaccc gatagtaata aacatcgcgc
     2401 cgcacttcat cctgcacggt gtctggtgat atcaccgcca cttctcccgg caggccgccg
     2461 tagatggagt aatcgtaggc agttattttc accagcgatt tttgccccgg gtggataaac
     2521 gccacatcgc gcggggagat ttttgcctca atcagcagtt gctcatccag cggcacgatg
     2581 gtcatcagtt taccaccggg ggcgatgacg ccgccaaccg tggtgacatc aatatcctga
     2641 acaatgcctc gcaccggcgc ggtaaagttg agcctggtga gagagtcttc ccgtccacgg
     2701 atcaccgaac gctgggtttc cacttccgca ttggcttttg ctaactcttc gcgcgcctga
     2761 acgtagtatt gcgtgcgcac gtcgctgagt ttgttttcca gttcattggc ctggcgttgc
     2821 aggcgcagta cttcgacgct gctcgctgcc ccctgtttta ccagcggctg ggtcatcgcc
     2881 agttcgcgat tgaccaggct aatcccctgc gtcagcccgg tggtggcttt gttcaacccg
     2941 tcgcggcgag tgttgtacaa cgccgtttcc gactcgataa gttcggggaa tttattcagt
     3001 tcgtcaggga agaccagcgg tttattgctc acttccgccc gcagccgcgc cgaggttgcc
     3061 agcgccgcgt acagccgcga catcgcttcc tggacgtcgg attccgtttt ggtgcggtta
     3121 agctgcgcca gcggctggcc ctgttcgaca atatcgccaa ctttcacgtc gagatgatac
     3181 agaattccgc cttccagcga ctgcaccacc tgttcgcgcc cggaaggttc taccgtgccg
     3241 ctgccggtgg tgacttcatc aagctggaag taccacgccc agccgagaaa acagagcagc
     3301 atcacagcgg taacgcgtac cgccagcgcc gaacgcggca ggcggggttc agtcagacca
     3361 cggttatatt gcagttgtcc gttcatgccg cagacccctt attcttctcc tgcaacacca
     3421 cccgtcgcgc tgtgggctcg ccctgttcac gcaaaatggc ctctttcgcg ccgtccatca
     3481 caatccgccc ctgatccatc acgattatcc tatccaccag ttgcagcatc gccgtgcggt
     3541 gagtggcgat aatcagcgtg cgatgcccaa tccactgttt cagttgttcg attaaatacg
     3601 cttctgccat ttcgtccagt gaggcgctgg gttcgtcgag caggacgata ttcggctgag
     3661 agatgagtag ccgcgccagc agcagcgttt gccgctgacc accagagagg ccaaatcccc
     3721 cttcctgaat catgtagttg aggccgtttt tctgcttctg cacaaagggc agcgctccgc
     3781 tgagggttaa cgcgcgttcg atatcttcat cgctggcttc cggcatcccc atggtgaggt
     3841 tttcccgcag cgaaccaaag aacagtcgcc cggtctggct gagtagcccc atgtctcgcc
     3901 gcagatctgc cggatcaagc tgcccgatac tgatgttatc cagcaacacc tgcccctgct
     3961 gggcgatacg cattccggcg agcatttgca gtagcgtcga tttacccgcg ccgttgcgcc
     4021 cgagaatggc gattttctca ccagcctgaa tgttgagttt gccaatcgct acatcggcaa
     4081 tcttctcttc ttcgtcgtaa taaaacaccg cgttgctgaa ctgataatta ccgtgcagca
     4141 ccgctttatg caccagtttg ccgtgttccg gctgatccac cgggcgcttc atcagttcat
     4201 caaggccgtt acgtgctact ttcgcctgct gccagcgcga gagcacgccg gatatctgcg
     4261 acagcggcgc aatggtgcgc gaagccagga tcgtcgtccc gaccagcgcg ccggtggtca
     4321 tgtcgccgtt catcaccata aagcagccca ccagcagcac caccacgtag acgatggact
     4381 gcacttcctg ggtccaggtg agcagcagcc cggtgagaaa acgttgcttc atgctgatgg
     4441 acgaggccac gtcgttggtg tggttccact gattctgaaa acgctgttcg gcgcgcagca
     4501 gtttaatatc ttcaatcgac tgcacggctt ccaccagcgt agcgttgcgt accgctgact
     4561 cgcgcattcc ttcgttcgcc agccgcgcca gcgggcgttg caccagcagg ccggggatca
     4621 ccagcaacag cagcgccagc aaaaccacca gcaccagcca gccgccgatc atccacaaaa
     4681 taaagacaaa cagcaggaag aatggcaggt ccgccaccgc gccgatggtg gtggacgtaa
     4741 taagctcccg cactgattcc agttcgcgga tctgcgagat aaacgatccg gtggatttcg
     4801 atcgcacgtt gtttttcagc cgtagcgcat gaccaaaaac gcgatccgaa atacgcaggt
     4861 ctgcccgctt tccgatcaca tcagatagat gcgtacgcac catgcgcata cagaactcaa
     4921 acaggatcgc catcatcacc ccggcgaaaa gcacccatag cgtcgggtac gattgcgccg
     4981 gaaccacccg atcgtaaacc tgcatcgaga aaatcatcgc cgccagcgcc agcacgttcg
     5041 ccaccagtga cgccagcata atatcgccgt agcgccgcca gtcttttagc gaaagactcc
     5101 agaaccagtt cgcctgatac gggcgaatat agtcatccac ccgcgcgtca gggatggcgc
     5161 tgagagggcg cagcagcgcc agttcgacga ttttatcttc aatatccgcg acgttaagtg
     5221 tagtttccag cccttcgtca ccgctgaact gaacgctgat attgtcatga ttgtcgcgac
     5281 gggtgatcac gccaatctgc tgattatcaa acaccgcgat cagcggcaga cgccaggggt
     5341 taagcgaatc ggcggaaaac tccaccaacc gcaaccccat ccctaactgc cgcgccatgt
     5401 cggtgagtaa ctcctcccgc ttactgtcgc gctcccagtt gacggtcgcg cgcacattct
     5461 cctgagaaaa atcgagccga taatagcgcg ccacggcgat catcgcctcc agccaggctt
     5521 ccacggactg ggcatttttt ttcattatct acaccaccat caactgatga ttcgccagca
     5581 aggtggcgag gtcggtgtgc acgcccgtca gcgtgaccac gttagttgtc gcaaaagtgc
     5641 cgcccgtgcc gtcgcggtcg atctgcacga tggtgtcgct gccgctctgg gtgactttga
     5701 caaagtcacc gatgtttccg gcctgcgcat ccagcgttgc cacgccgttg acgtagctgg
     5761 ctttgccgtt gccggtgtag ccgctgccct gtaacagttc acgaatatca atgcgatcgg
     5821 tgtcggcggt gccttcccat gtccctaccg taaagccatt caccacgtct gagccgttgc
     5881 cgccagtggc gtcgctggcg ttgagcagtt tatagagcaa ggtgtcgtgg ccgccgttgc
     5941 cgatgttgag cgtgtcgttg ccgccgcgcc cttccagttg gttatcgccg ctgctgccgg
     6001 tcagaatgtc gttaaaattc gagccggaaa tgccttcgat attggtaaat gtcgaggtgt
     6061 taaaaccggt ggcctgcgcg gcagtgctgc ttaagttagc cgtcacgccc accgtcgaaa
     6121 gccggaaatc aaccacgtcc atcccgccgt tcgccagcca cgttccggtg ccggagctgt
     6181 acacccagcc gccggaaccg tcgtaagtgt cggtgcctgc ggtggcgaag aaggtgtcgt
     6241 tatagccggt gccgacgaac ttgccgttgt tgctcgcggt acccgcttca gcgctgagat
     6301 cgtaagcgtg agtggcatcg gtggcctgta aaccgcccca gctggtgttg accgtctggt
     6361 cgatattgct ggcggtggtg ccggtggatt tgatcagcac cgcgttgtag gtttctccgg
     6421 cattcagccc gtagaagttg accagcgccg aggtgtcgtt aacccccata cccgactgcg
     6481 ggttgatgat ttgcgtggcg accagcactc ccgccgagtt gtacagcttc acggtattgc
     6541 cgtaatagac gttaatgccg ttgggatcgg tgatgcgcag gtgtagcgaa gtgccgtagc
     6601 tcaccgtgtt ggtgttgcgg ctcaggaaca cgctgcccga ctgtttggac accagcacat
     6661 ccaccgcgcc gtcccagtcg tagtccattg ccgccacgcc gctggtggta ccgctctggc
     6721 tgccgccgag ggccgattgc gtccagttgc tggcgttgct gacgttagta taaagatacg
     6781 actgcccggt ctgggcgata cgggcgatat ccatcaggcc gtcgtggttc cagtccaccg
     6841 ccaggctaaa ctggctggcg taggtggttg ccgttgcgcc caccgccacc gggctaccca
     6901 gtacgccgtt ggtgttgaat aacagcgagc cgtagttagc tcttccctgg ctggcgggca
     6961 ggaagagatc catatcgccg tcaccatcga aatcggccca ggtcatggaa acgctgctgc
     7021 tcatagcgcc actgcccgcc ccgctcagga aggtattggt ggtgttctgc ccccagacaa
     7081 acgtcccatt cccctggttg atgagcgaag acagcgtgta atagttgttc aggttatagg
     7141 tgtgctgaac caggtcgatc ctgccgtcat tgttcagatc gacgccagaa ccttcgttga
     7201 gcgaaagata ccccgtcacc gccccgccca ccgtggcgct accgccgctg ttcgacgtgg
     7261 tggagttgcc taccagcgtg cctgcgttgt tccacaggaa ggtgttggag tccggcccgc
     7321 cagcgtcacc aatccagaag tcgagatagc cgtcgccctc tttatcaaat gccacaatcg
     7381 agccgtacca cgtcagggtg ccgacggtga cctgaatagc gctggaaaag gtgccatcgc
     7441 cgttattggt aaagccgtta atatagcccg taccgtagtc atcacgggtg ataaacagat
     7501 cgcccgtgcc gttacggtcg taatcggcaa tggagctggt ggcgtagttg ttgccgctct
     7561 gatacagcgt cagcgcggtc ttcgaccagg tgagtgggtc agtcgattgc attacctgct
     7621 ggtttgccag caccgtccac atcccgtgcg agtcgaggcc gtaggtcagc ccccaggcgg
     7681 tggttttgct ggcggtagtc caggtcggtg tgtaatcaat cgccgcgtta accgtcaccg
     7741 tgccgttgct aatattggcg ttattgccgt tacccgccga actttttacc tgcgcagtaa
     7801 cggtataggc ggtcgcggaa actgtcagcg catcggtatc cggcaactgt acataccagg
     7861 tgttgtgcgc cggatcgacc actaccgcgc cgcccggttc agaggtgtag gttttgccgt
     7921 tgatcaccac ttcaacatac tgcccactgg caagcgcggc ggtgatcacc ccgctatagt
     7981 atcgcgcgtg gtctggctgg tgatttgcgc cagcgtggtc ggaatagagg tatcgaccgt
     8041 caggacaaaa tcactggagg aggtgatatt ccccgccaaa tccaccactc gcgcgctgta
     8101 ggtataggca ccgctcacca gcccgctgtc gctgtaggtc cagttcagag cgccgaccat
     8161 cgtcaccgcc cctaacagca gcccgttacg gtagaggtaa accacttcac cgctggcaag
     8221 cggcgcggaa agtacaccgt tcagcagcgg cgtagtgtcg tcggtggcct gcgaactgct
     8281 taatgtcccc tgccgctgcc cgacatcatc ggtatacgac acaatcgttg gtgtggcggt
     8341 ggggttaatc gtatcgacca ccaccgtttt cgacaccacc gggccggtgt tccccgccag
     8401 ctccagcacc cgcacctgat aaacgtagct gccgtcggtc agcgtgcggc tgtcggtgta
     8461 agtccactgt gtaccgttgg tggtgagcgt tgtccaggtc gcgccgctat caaggctaat
     8521 ctgcgccact tcgccgctgg caagccccgc ccccagcgaa ccggtcagcg tgagcatggt
     8581 gtcgctggtg ataaagtcag ttgccgaact ccccgtgtcg gtgctgatgc tggcgatggt
     8641 gagcacctgc gccgggttaa cggtatcgat cagcgccgac tggctgctcg ttgcgccaac
     8701 gttgcccgcc agatcgacca cccgcacctg ccaggtggtg gtgccgttgg tgagggttcg
     8761 tccgtcaacg taactccagt tcaggctgtc tgcggcgacg gtcacgttca cccaggttgc
     8821 gccgttgtcg gtactgattt gcgcgaactc attagcgccg agcgccgcgc ccaatacgcc
     8881 gcgcacggta agcgtggtgt cgctggtaat aaaatcgctg ctgctggttc cggtgtcatc
     8941 gctgataccg gtaatggtga tacttttcgc cgcttccggg ctggtggtgt cgaccaccac
     9001 gttttgcgtt gcggtctgcc caacgttccc cgccagatcc accacccgca ccgtgtagtt
     9061 ccaggtgccg tcagtaagcg tgtgaccatc ggcatagctc cagctggtgc caacgacggt
     9121 taacgtagtc caggtgacgc cgccgtcgag gctgatttgc gcgaactcac cggcagaaag
     9181 cgtcgccccc agcgtgccgc tcaccgcaag cgtcgtgtcg ctggtgacaa aatcgttagt
     9241 gatcaagccc atatcggtgg tgatcgcgct gatggcgatg gttttcaccg cgggatctgg
     9301 cgcggtagtg tcgatcacca cattctggct gtcggtcgcg ccaacgttgc ccgctgcgtc
     9361 aatcacccgc acctgataga gatagttgcc atcggtcagc gtgcggctgt cgttatagcg
     9421 ccaggtcgtg ccggtaacgg taagcgtggt ccaggttgtg ccgccgtcga tacttatttg
     9481 cgccgtctcg ttactggcaa gcgcggcggt gagcgagccg ttgaccacta ccggactgac
     9541 gctggtgata aagtcgctgg cgctaaggcc agtgtcggct tgcagggaat cgatattaat
     9601 gcccatcgcc ggagcgctga gatcgacggt aatggtgtga ttactgctac cggtgttgcc
     9661 aatggcattg ctcaccgagg cgttaatcac gtaggcgttg ccgtccgcca gcgcggtgac
     9721 atcggcgctg ccgagggtgt agctccagct accgcccgtc tgcaccgtgg tggtgtaggt
     9781 tttgccgttt aaggtcaggg tcagcgtctg cccgacgggc gcggtggtgg tgccgctgat
     9841 caccagcgat gagccgtgtt ccgctgcatt caccacatcg tcgcccgcaa aggtgttgat
     9901 ggtgacagta ggtacatcgc cgttcagcgt cacgccacgg ctggcgctgc cggggtttcc
     9961 ggcctgatcg ctaaccgtcg cgctaatggt gtagctgccg tcgcttaatc cggcaaactg
    10021 ctgcgccgga ataaacaccg accagcttcc gccgctgcca accgttgcgc tccaggtttg
    10081 cccgttaaag gtaacggtca ccgtctggcc cacttcggcg gtggtggtgc cgctaatggt
    10141 ctgcccggca agctgctcgg cgttgttgat aatatcgtca cccgccaccg tggcgatggt
    10201 gaccgttggc gcaacggtat cgacggtcag ggcgtgcgtg gcctgtccgg ggttaccggc
    10261 gcgatcgttc acgctggcgg taatggtctg cgcgccctgc ccgagcgcgg ccagatccac
    10321 cgcaggcacg ttcagcgccc aggtaccatt ggcggcaacc gtggcggtat aggttttgcc
    10381 gccaaaggtg acggtgaccg tctgccccac ttccgcgctg gtcgagccgt taagggttaa
    10441 cggctgctgc tgttcggcgg cgttgaggcg gtcgtccgtc gaaacggtgt taatgctgat
    10501 aaccggcgcg gtgagatcca ccgccacgct gcgcgaggcc gtggcgctgt ttccggcact
    10561 gtcctgagcg gaggcgctaa ccgtgtagct ggtgccatca ctgagtgccg caacatccgc
    10621 cgcaggcacg ttcacgctcc agctcccgtt gctctgaatg gtggcgctgt agcctttgcc
    10681 gttaagcaac acggtcacca ctgtacctgt cgcgaaatta gtgccagtgc cgctcagggt
    10741 cagcgcactg cccgcttcgg cggcgttaat aaggttatcg ccgctgatag tgctgaccga
    10801 gagcgacact gccgccgtgt tcacctgtac gttgtgcgtc tgcgtgctgc tgttgcctgc
    10861 gctatcggta atggtggcgc tgatggtcac cgtgccatcc gcaaggccgg aaatcacgct
    10921 ggcaggcacg ccgacgctcc agttgccgct ggcatcggtg ctggtgacat actgctgccc
    10981 ggcgatggtc accaccaggc ggtcgcccgc caccgcgccc gtggcggtgc cgctgataat
    11041 ctgcgcctga atgtgttcga cgttgttaat cacatcatcg cccgccacgg tgttaaaact
    11101 gatcaccggc gcagtggtat cgacggtcac gcctttgctg gcgctgccaa ggttacccgc
    11161 cagatcgctg accgtggcgg tcagggtgta gccgttatta gcaagtgcgg ttaagtcgct
    11221 ggcgggcagc gttaaactcc agctgccgtc tgtctgcacc gtggtctggt agttagtgcc
    11281 gttaagcgtg acggtgagcg tctgcccggt ttgcgcggtg ctggtgccgg acaccgttac
    11341 gcccgccgcc gcttcgctgg cgttgatgat gttatcgctg gcaatggtgt tgatggtcac
    11401 cgttggcgcg gcgctatcga cgctgtattc gtgcgttgct gtggcgctgt tgccgttgac
    11461 gttagtcacc cgcacctggg cgctggcatc gccatcacgc agggccgcca aatccgccgc
    11521 cggaacggtg gtgctccagc taccgtttgc cgccaccgtg gtggtgtagg ttttgccgcc
    11581 aaaggtgacg gtgacggttt gccccgcttc gacgccggac gtgctgccgg agagcgttaa
    11641 cgccgcgcct ttttcggcgg cgttaatcgc atcatcgctg gtgatgctgt tcatgctgat
    11701 ggcgacggcg gtgagatcaa cagtcagcgg atgggtaatg ctggtttgcg ttccggcact
    11761 gttcgccccg ctaacggtaa tattcagcgt tcccgcaggc cagttactga catccgtcgc
    11821 aggaacgccg acgctccagc taccgttcgc caacaccgtg gcggcgtaag tttggttgtt
    11881 aatcaccacc gtcaacgccg tgcctgcggc aaagccggag ctgctgccgg tgatcacctg
    11941 cgcctgagcg tgttcgatga tattgaccac atcatcaccc gcgacggtat ccacccgcag
    12001 gccaggcaga ctggcatcga tggtgatatc gcgcgtgccg ctaccggtgt tgccaacggc
    12061 gttggtcacc gaggcattaa ccgttaactc accgttaccc agagcctgcc agtcggcggc
    12121 aggtacgctc acattccagc ttaaatcagc ctggacggtg gcggtgtaag ttttaccgcc
    12181 cagttcaacg gtgacggtgc tgcccgccgc cgcaccggtt acctggccgc tgatagtttg
    12241 cgcgctgcct gcctctgaag cgttgataat atcgtccgtc gccaccggat taatggtgat
    12301 gcccggcagc gcggtattaa cctgcacgtt atggctggcg gtgctgctgt taccctgagc
    12361 attggtgacg ctcgccgtca cgctgtaggt cgcttcgccc agcgcactga ccgccgaggc
    12421 aggcacggta acactccagt tgccggaggc atccgtggtg gcgctgtagt tttgtccgtt
    12481 cagggtgacg gtgatcgccg tacccgcagg ctgatcgctg gtgccagaaa gcgccagatc
    12541 agatcctttt tccgtcgcgt tgatcacatc atcgcccgcg atggtgttga tgctaatcac
    12601 tggcgcgctg aggctgacgg tcacggtgcg gctggcgtcg tcgctgttgc ctgcccgatc
    12661 gctgacgctg gcggtgatgg tctgcgcacc gctacccagc gccgtaacat ctgccgccgg
    12721 aacgccaacg ctccagttgc cggaggcatc cagggtggtg gtgtaggttt tgccgttgag
    12781 cacaacgctc accacatcgc ccgcttcccc gccgctactg gtgccggaga tcaccagcgc
    12841 ctgcgcatgt tcggcatcgt tgatgatgtc atcgcccgcc acggtgttaa tggtgagaac
    12901 cggcgcggtg gtgtctaccg ccagattatg gctggcgctg ccggggttgc ccgctttgtc
    12961 attgaccgtg gcgctgacgg tgtaattgct tgcgttgagc gcgcccaggg ctgaagtggg
    13021 aacgctgacg ctccagctac cgtccgcctg cacagtgccg gtgtaggttg cgccgttaag
    13081 ggtgacggtc accgtctgcc cggtttcggc ggtgctggtg ccgctgatgg tgagcgggct
    13141 tccggcttct gcggcgttaa ggatatcgtc gctggcgatg gtgttaatgg tgagcgtcgg
    13201 cgcgctggcg tcaacgctat acgcatgggt cgccgaggca ctgttaccgc tcgcgctgct
    13261 aacgctggcc tgcacattcg ccgcgccctc tggcagagtt gccagatctg ccgccggaac
    13321 gtttacactc cagctcccgt tggcggcgac gcttgcagtg taggttttac cgccaaaagt
    13381 gacggtgacg gtttgcccgc tctcaatccc gctggtactg ccggaaagag tcagattggt
    13441 gcctttttct gcggcgttaa tcacgtcgtc gctggcaacg gtgttgatgc tgattgccac
    13501 cgccgttaaa tcgacggtga acggatggct gacgctgact gggttattgg cgctgctttg
    13561 cccgtctacc tccacggtta acggccccgc aggccaggcg ctgacgtttg ccgccggaat
    13621 gccaacgctc cagcttccgt ccgcctgcac cgtggcggaa tacgccacac tgttgatggt
    13681 aaccgtcagc acagcacctg cattcaggcc gctactgccg ccggtgatca ccagcgcctg
    13741 cccgtgctcg atgctgttga cgatatcatc gcccgccacg gtatctacgc gcagccccgg
    13801 caggttggca tcaatggtga tatcccgcgt gccgctcccg gtgttgccat tagcgttggt
    13861 gaccgaggcg gtaatggtca aatcaccatt gcccaacgcc tggagatcgg ctgtcggaac
    13921 gctgacgctc cagcttaagt tgctctgtac cgtagcggtg taagtgttcc cgcccagcgt
    13981 cacggtgacc gtatcgccag cggcggcgcg agtcaccacc ccgctgatgg tttgatcggc
    14041 ccccgcttcg gcggcgttga taatatcgtc gcctgccacg gtgttgatgg tgacgtccgg
    14101 caggccgctg tcgaccagta aattcgcctg gctgctggcg gtgttgcctg ctgcactggt
    14161 ggcgctggcg ctgaccgtat agttggcctg acctaatgcc gccagatcgc tcaccggcac
    14221 ggttaaggtc cagttgcccg atgcgtcggt ggtggcgctg tagttgtgac cgttgagcgt
    14281 gacggtgacc tgcgcgcccg tcgccagccc cgtactgccg ccgctgatgg tcaacggctg
    14341 gcctttttca tcggcgttca ggatgttatc gccgctgatg gcgttaaagg taatggtcgg
    14401 cagcccggta ttgaccgtca cctgatgggt agcgctgccg ctgtttcctc cggcatcggt
    14461 gacgctggca ttgatggtca ccgtgccatt cgccagtgcc gagacgacgc ttgccggaac
    14521 gccgacgctc cagttgccgc tggcatccag caccgtggta aaggtatttg tgccgatagt
    14581 caccgtcacg gtgctaccgg ttgccgctcc agtggcggag ccgctgatga tctgcgcctg
    14641 ggcgtgttcc gtcgcgttga tcacatcatc gcctgccacc gtgttgatgg tgacgaccgg
    14701 aacggacgta tccaccgtca ggttgtgatt aaccgaggcc gggtttccgg ctttgtcgct
    14761 caccgccgcg ttcacggtgt agttgctggc ggtcagggtg cttaagtcgg ctgacggtac
    14821 gctgacgctc cagctgccgt ccgtctgtac ggtgccggtg tagtttgtgc cattgagcgt
    14881 gacggtcacc gtctgccccg cttccgccgt gctgctgccg gtgatggtca gagccgctcc
    14941 ggcttcggcg gcgttaagaa tatcatcgcc cgcaatggta ttaatggtca ccgttggcgc
    15001 gctggcatca acgctgtaag cgtgggtcgt ggtggcgctg ttgccgttaa cattgctgac
    15061 gctggcctgt gcgctggcat cgccatcacg cagagccgcc atatctgccg ccggaaccga
    15121 ggtgctccag gaaccattcg cagccaccgt ggcgctgtaa gttttgccgc caaaggtgac
    15181 ggtaaccgtt tgtccggctt caacgccaga ggtgctgccg gagagcgtta acgccgcgcc
    15241 tttttcggca gcgttgatca catcatcggc ggtaatggcg ttgatgctca ccgccaccgc
    15301 cgagagatcg accgtcaccg gatgcgtaac gcttaccgga tttccggcag aggtgctacc
    15361 gctcgccgca atcgtcaccg atcccgcagg ccaggcgctg acatccaccg ccggaacgcc
    15421 gacgctccag gtgccatccg ccagcaccgc cgcaacatag gtttgcccgt taatggtcag
    15481 cgtgacgttg ctgcccgccg ccagaccgga gctgctgccg gtaatcacca gcgcctgccc
    15541 gtgctcgata atattaacca catcatcgcc cgccacggtg tcgacccgca gaccggggag
    15601 attcgcgtct atggtgattt cgcgcgtgcc gttaccagta ttgcctacgc tgttcgtcac
    15661 cgaagccgaa atggtcagtt cgccgttgcc caactcctgt agcgcggagg ccggaacatc
    15721 gacgctccag cttaaattcg cctgcaccgt ggcagtgtaa gtcgccccgc cgagcgtgac
    15781 ggtcaccgtg tcgcctgccg ccgcacccgt cacctgcccg ctgatggtct gttccacgcc
    15841 cgcttcggcg gcgttaataa tatcgtccgt tgccaccacg ttaatggtga cgcccggcag
    15901 cgcggtatta acctgtacgt tatggctggc ggaaccgctg ttgccatcgg cgtcagtggc
    15961 ggctgcggtc accgtgtagg tggcttcacc gagggcgctc acccgtgacg ccggaacggt
    16021 cacgctccag ttaccggaag catcggcagt ggtggtgtaa ttttgcccgt tgagcgtaac
    16081 ggtaatctgc gtgcccgcag gctggttgct ggtgccggta atcgccagat ccgcgccttt
    16141 ctccgcggcg ttgatgatgt catcgacggc aatggtgtta atggcgagca ccggagcgcc
    16201 gagcgccacg gtgacagtgt gcgaggccgt gccgctgttg cctgcggagt cggtgaccgt
    16261 agcggtaatg gtcacatcgc cctgcgccag cgcggaaatc acgctggcag gcacgccgat
    16321 gctccagttg ccgttggcgt ccagcacggt ggtataggtg gtcgtgccaa tcgtcacgga
    16381 aaccgtatta ccggtagtcg cgcccgttgc cgagccgctg atgatctgcg cctgtccgtg
    16441 ttccgtggcg ttaatgatgt catcgcccgc caccgtgttg atggtgacga ccggagcggc
    16501 aaggtccacc gtcagattat gcgttgccga agccgaattt ctggctttgt cgctgaccga
    16561 ggcgttaacg gtatacgagc tggcggtaag gctagctaaa tcgccggttg gtacgctgac
    16621 gctccagctc ccgtctgcct gcacattgcc gctgtaatta acgccgttta gcgttacggt
    16681 caccgtctgc ccggcttccg ccgtgctggt gccgctgatg gtcagcgccg atcccgcttc
    16741 ggcggcgtta agaatatcgt ccgtggcgat ggtgttgatg gtaacggagg gcgcagtggc
    16801 atcaacgctg taagcatgcg ttgcctgggt gctgttccct gcgacattgc tgacgctggc
    16861 ctggacgttt gctgcgccgt ctggcaaggt tgcaacatcg acggcaggga tcgtcagccc
    16921 ccaggtatta tccgcggcaa ccgtggtggt gtagcttttg ccgccgaaga taacggtgat
    16981 ggtttgcccc gcttccacgc cggaggtggt gccggagagc tgcaaatcag agcctttctc
    17041 ggcggcgtta atcacatcgt cagtggaaag ggtgttgatg gtgatcgcca cggcagcgag
    17101 atcgaccgtg accggatggc taatgctggt tgtggttccg gcggtgttgg tgccagagac
    17161 ggcaatattg accgtccccg caggccagtt tgtgacatct gccaccggaa caccaacgct
    17221 ccagctacca tccgctaaaa ccgttgcgcc gtaggtgacg ctattgataa ccaccgtcaa
    17281 cgccgcgccc gccgccagtc cgctactgct accggtgatc accaacgcct gagtgtgctc
    17341 gatgctgtta atcacatcat cgcctgccac ggtatcgacg cgcagacctg gcaggttggc
    17401 atctatcacg atatcgtgcg tcgcggtgcc ggtattattt gcggaattgg tcaacgaggc
    17461 gctgatggtc agttcaccgt tacccagcgc ctgcaaaacg ttcgccggta cgctgacgct
    17521 ccagcttaaa tctgactgca cggtggcgtt atattgattg ccgccaatgg tgacaatcac
    17581 cgtattcccg gcaaccgccg ttcccgtgac ctgaccgctg atggtctgcg ccacaacgat
    17641 ttcagcggcg ttgataatgt catcgccgga gacggtgtta atggtcagcg tcgggccgct
    17701 aagatcaacg gtcacatcgt gagtcgtgct gtcgctgttg cccgcgcgat cgctcaggct
    17761 ggcggtgatg gtctggctac cggtcgccag cgccgtgaca tccgccgccg gaatgcccac
    17821 gctccagtta ccggaggcat ccagcgtggt ggtgtagttt ttaccgttca gcgtgacggt
    17881 taccacatca cccgccgccg cgcccgtact ggtgccgctg accaccaacg cctgatcatg
    17941 ttcgatggcg ttgataatgt catcgcccgc gacggtgtta atggtgagat ccggcgcagt
    18001 gatatctacc accagcgcgt ggtcggcgct tgccgggtta cccgctttgt cgctcaccgt
    18061 ggcggtcacg gtgtaactgc tggcggttaa tccgcttaaa tccgctgccg gaacattcac
    18121 gctccaggtg ccatccgcca gaacggtggt ctggtacgtg ttgttattga gcgtgacggt
    18181 gagcgtctgc ccggcctgcg ccgtggtagt gccgctgatg gtgatccccg cgccagcttc
    18241 gctcacgtta aggatatcgt cgctggcgat ggtgttgatg gtcacaagcg gcgcggtggc
    18301 atcaacgcta tacgcgcgat cggcctgggc gctgttgccg ttaatattac tgacactcgc
    18361 ctgcacgttg cccgcgccgt ccggtagcgc agcgagatcg gcaggcggca cattcaccgt
    18421 ccagctaccg ttagcttcca ctgtggtggt gtaatttttg ccgccaaagg tgacggtcac
    18481 cgtctgcccg gcttcgacac cgctggtgct gccgcttacg accagcgttt cgcctttttc
    18541 gacggcgttg atcacatcat caccagagag agtgttaatg gtgatggcga ccccagccag
    18601 atcgacggta accggatggg taatggtgga ggttgttccg gcactgttgg tgccgcttac
    18661 cgtgatattc accgtacccg ctggccagtt gctgacgtct accgccggaa caccgaggct
    18721 ccacgtgccg tcggctaata ccgtcgcgcc ataagtaacg ttgttaatct cgaccgttaa
    18781 cgccgttccc gccgttaatc cgctgctgct gccggtgatc accagcgcct gattgtgctc
    18841 aatgctgtta ataacatcat cgcccgccac ggtatcgacc cgcaggccag gcagattggc
    18901 gtcgatcgtg atatcgcgcg agccgctgcc ggtgttgccg acgccattgg tgaccgaggc
    18961 attaaccgtc aggtcgccgt tgcccagcgc ctgaatatcc gccgccggaa cgtccacact
    19021 ccagcttaaa ttagactgca ccgtggcggt gtaggtgttg ccgcccagcg taacggtaac
    19081 cgtatccccc tgcgctgcgc ccgtcacctg cccgctgatg gtttgcgcat ttcccgattc
    19141 ggcagcgtta ataatatcgt cagtcgccac cgcgttaatg gtaacggcag gcaaggcgct
    19201 gttgaccagc acattatgac tggcggaatt actgttgcct gccgtatcag tgacgtttgc
    19261 cgttaccgtg tagttggctt cacccaatgc gctaaccgct gacgctggca ccgtggcgct
    19321 ccagttgccg ttgctatcgg tagtagcggt gtaattttgc ccgttcagcg tcaccgtaat
    19381 ggtggtgccc gcaggctgat tactggtgcc ggtgatttgc aggtccgcgc ctttttccgt
    19441 ggctttaatc acgtcatcgg tggcgatggt gttgatacca atggttggcg cggcgagatt
    19501 cacggtgacc gtgcggctgg cgtcatcgct gttgcctgcc gcgtcggtaa ttgccgcagt
    19561 gatagtttgc gggccgctgc caagcgcagt gacgtcagcc gccggaacgc cgacgctcca
    19621 gttgccggaa gcgtccagca tggtggtgta ggttttactg tttagtgtga cggtgatgac
    19681 atcacccgct tcgccgccag tgctggagcc ggagatcacc agcgcctgtc cgtgttcggc
    19741 ggcgttaata atgtcatcgc cggagacggt gttgatggtc agcaccggca cggtgagatc
    19801 caccgccagc ccgtggttag cggacgccgg gttacccgct ttatcgctta ccgaggcact
    19861 aacggtgtac tggctggcgg tcagattgct gagatccgcc gtcggtaagc tgacgctcca
    19921 gctgccgtcc gcctggacgg agccgctgta agtcacacca ttaagcgtga cggttaccgt
    19981 ctgccccgct tcggcggtgc tgctaccgct gatggttaac ggattgcccg cctcggcagc
    20041 gttcagaata tcgtcggtgg cgatggtgtt aatggcaagc gtcggggccg tggcatcgac
    20101 gctgtaggcg tgggttgccg aagccgtgtt gccgttaata gtgctgacgc tggcctgcac
    20161 ggtggcgtcg ccgtcgcgta acacgctgag atcggcggcg ggtacggtgg ttgtccagct
    20221 accatcgccc gctacggtag cggtgtaggt tttcccgcca aaggtaacgg tgaccgtttg
    20281 ccccacttcc acgccggagg tgctgccgga aagggttaaa tctgcccctt tttcggcggc
    20341 gttaatcaca tcgtcgccgg aaacggtgtt aatggaaatc gccaccgccg ccagatcgac
    20401 ggtgaccgga tgggtgatgg tggatgttgt tccggctgtg ttagtgccac ttaccgtgat
    20461 attcaccgta cccgcaggcc agttactcac gtctgccgcc ggaacaccaa cgctccatgt
    20521 gccatcggct aatactgttg cagcgtaagt gaccgtgtta atcaccaccg tcagcgccgc
    20581 gcccgccgcc agcccgctgc tgctaccagt tatcaccagc gcctgagcgt gctcgatgct
    20641 attgaccaca tcatcgcccg ccacggtgtc aacgcgcaga cctggcaggt tggcgtcgat
    20701 agtaatatct cgcgaaccgc tgccagtatt gccaacgccg ttggtcaccg aagcgttaac
    20761 cgtcagattg ccattgccga tggcctgaat atccgccgcc ggaacgtcca cgctccagct
    20821 taaattcccc tgcacggtag cggtgtaagt tttcccgcca agcgttacgg taaccgtatc
    20881 gcccgccgcc gcccccgtca cctggccgct gatggtttgc gcattgcccg attcggcggc
    20941 gttaataata tcgtcggtcg caactgggtt aatggtgacg ccaggcagcg cggtattgac
    21001 ctgcacgtta tggctggcgg aattgctgtt gcctgcgctg tcggtgacgt tcgccgtcac
    21061 cgtgtagctg gcttcgccca gcgcacctac tgcggaagct ggcaccgtag tgctccagtt
    21121 gcctgcagcg tccgtcgtcg cggtgtaatt ttgcccgtta agcgccagcg taatcgtggt
    21181 gcctgctggc tgattactgg tgccggaaat ttgcagatcc gcgccttttt cgctggcgtt
    21241 aattatgtca tcggtggcga tggtgttgat gtcaatggtt ggtgcggtga ggttaacggt
    21301 gaccgtgtgt gtctaattgt cgctgttgcc tgccgcatcg gtaaccgtgg cggtgacagt
    21361 ttgcgggccg ctgccaagcg ccgtgacatc cgccgccgga acgccgacgc tccagttgcc
    21421 ggaggcgtcc agggtggtgg tgtaggtttt actgtttagc gtgacggtga cgacatcccc
    21481 cgcttctccg ccggtgctgg aaccggaaat caccagcgcc tgcccgtgtt cggtggcgtt
    21541 aataatgtca tcgcccgcaa tggtgttgat ggtcagcacc ggaacggtga gatccaccgc
    21601 cagattatgc gtggtgctcg ccggattgcc cgctttgtca ttgaccgtgg cggtcagggt
    21661 gtggctgcca tcggtcaatg cctcaagatc tgccgccgga atattgatgc tccagctgcc
    21721 gtccgcctgc accgttgcct gataggtctg cacggtcggg ctgttaagcg ttatcgtaac
    21781 aatctgcccc gcttcggcgg tggtactgcc gctcaccgtc acgcccgcat cggcttcgct
    21841 ggcgttgacg atattgtcgc tggcgacggt attgataatg atggttggcg cgctgctgtc
    21901 gacgctgtag ttgtgcaccg ccgaggcgct attgccgttg atattgctga cgctggccag
    21961 tgcggaggcg ctgccctcgg gtaatgacgc cagatcggcg gcgggtacgg tggcagtcca
    22021 gctaccatcg ctcgctacgc tggcagtgta atttttcccg ccaaaggtga cggtgacggt
    22081 ttgaccgggt tctacgttag tggtggtgcc ggaaagggtt aaatcagcgc ctttttctgc
    22141 ggcgttaatc acatcgtccg tggcgatggt gttgatggtg atcgctgccg gagtgagatc
    22201 caccgtcacc ggatgcgtaa tgctcaccga gtttccggcg ctactttccc ctgaaacggc
    22261 aatattaacc gtccccgcag gccaggcgct aacctgcgcc gccgtgacgc ccacgctcca
    22321 gctaccatcg gcctgcaccg cagtggtgta ttccacatta ttaatcgtaa cggtaagcgg
    22381 cgtgctttca gccagccccg agctgctgcc ggtgaccacc agcgcctgcc cgtgctcgat
    22441 gatattgacc acatcatcgc ccgccaccgt atcgacccgc aggccgggca gattggcgtc
    22501 gatggtgata tcccgcgtgc cgctgccggt gttgccgttt tgattggtga ctgaggcatt
    22561 aaccgttaaa tcgccatttc ccagcgcctg aatatctgcc gctggaacgt ccacgctcca
    22621 ggtgagattg ctgcccaccg tcgccgtata agtattacca cccaacgtaa tagttaccgt
    22681 gtcgccatct tccgcgccag tcacctgccc gctgatggtt tgcgccacgc ccgcttcggc
    22741 ggcgttaata atatcgtcgg ttgccaccgg attaatggtc acaccgggca gcgcgctgtc
    22801 gaccagcacg ttatggctgg cagtggcgct gttgccgata tcgctggtca ccgccgccgt
    22861 taccgtatag ttggcctggc ctaatgctgt aaccgccgat gccggaacgg tgacgctcca
    22921 gttgccggag gcgtcggtcg tggtggtgta attttgcccg ttcagcgtca cggtgatggc
    22981 ggtgttaaca ggctgatcgc tggtgccggt aatctggaga tcggcccctt tttcgctggc
    23041 gttaatcaca tcatcgcccg caatgctgtt aatgccgata aggggcgcgg cggtattgac
    23101 cgtgacggtc aatgactggc tgcccgtgtt tccggctttg tcggttaccg agacgctgac
    23161 aggataactg ccgtccgcca gcccactgac caccgaggcc ggaacgccca gactccagtt
    23221 gccggaacca tccaccaccg tggtgtaatc cacattattc agcgtcacgg tgacgatatc
    23281 gcccgcctga gcgccagtaa ccgtgccgct gatgatttgc gcctgagcat gttccggcgt
    23341 gttgatgacg ttatcggtcg ccaccggatt aatggttacc accggcggcg tggcatccac
    23401 cagcgccacg cgtgaaacgc tgctcggatt accggcgaca tcgttcacgc tggcggtgac
    23461 cgtagcgttg ccgtcagcca gtgcgcccac gttggcggcg ggcacagtca cgctccaggt
    23521 gccgtctggc tgcacgacgc cctgatacgt ctgcccattt aacgtaacgg tcaccgtctg
    23581 ccccggctga gcgtcggtgg tgccggtgat gggcagatcc tgcccgcttt cggcggcgtt
    23641 aatgatgtta tcagcggtca gtggatcaat gcttaaggcc ggggcctggc tatcgacggt
    23701 aatggtgcgg gaagtgttgc cggtattgcc gctgagatcc gtcacagaag cggtaatcgc
    23761 caccgcgccg tcaggcagat ttcccatcgc ggcggcaggt accgtcagac tccaggagcc
    23821 atcggcctgc acggtagtgg taaaactctg tccagccagg gttactgtga cggtttgtcc
    23881 tgtctcagcc tgttgagtgc taccggtaag ggtcagtggc gcaccttttt ctgcgccact
    23941 gataatgtca tcgccggaga cggtgttaat ggtgatcaac ggagaaacac cgaccagcgt
    24001 gattgctcga gacgccgcgc cagtatttcc tgcagcatct gtcacggtga cgaaaatcgt
    24061 attggctccg cgatccagcg tgcgggttac cgctgggtcc agcgccacat tccagctgcc
    24121 atctgccagc acgatgcccg tcaggacatg agtgcccaat ttcaccgtta ccgtatcgcc
    24181 tggagaagca ccgctgactt gtccggagat gatctgcgcg acggcctgct cggcattatt
    24241 aagaatatcg tcgcccgcca cggtattaac actgaccacg ggtgctgagg tgtctaccgt
    24301 gaaattcgcg ctgtggctgg tggtgttccc tgcccgatcg ctgacactgg cattgacagc
    24361 gtaattaccc tccgccagtg cctggacttc cgtggcaggc agcgtcactt gccaggttcc
    24421 gtcgctaccg acggttgctg tatggctttt cccgttgacg gtcagtgtta cggtttgccc
    24481 cgcttctgca ttcgacgtgc cgctcagtac cagcgcgacg ttatgttccg cagcactgat
    24541 aatgttgtcc tgagcgatgg tgttgatggc aagcgttggc ggttgggtat cgaccgttag
    24601 tagctgcgcg ccggtgaccg tattgcctgt ggcgtctttc ccactgacca gcaccgcctg
    24661 attcccttcg cctaacgcct gtgcatcggc ggtcggcacc tgcacgctcc aggcaccgtt
    24721 agcgcctacg ctggtcgtgt aggttttgcc gtttagcgta acggtgagtt ctgttccctg
    24781 cgccagatgg ctactggtgc cagaaagcgt aaacccggcg gcctgttcgc tggcgttaat
    24841 aatgttatcg cctgcggtgg tggcaacctg cacgctggcc tgactattgt cgatggttaa
    24901 cgttatcgaa ccggcgtcag tgatggtact gccgacaatt tgcaccactg accaggtatg
    24961 ttcacctgca ctttgcgtgg gtaacgagac actccagtga ccgttgttgt ctacctgagt
    25021 actggcaata gtattgccgt tactgtcttt aatctgaata gtcgcccccg cctgcccgct
    25081 gccgctaaag gttggcgtgg tgtcatcggt gatgtcattg gtggctagaa tgccgcgctg
    25141 atcgccctga ttatcggtaa ctaaaaatga tggtgtggcg ttgccaggct cagcaggtgg
    25201 cgtagggtta ttaatcactt ctgttttcga gtcgccatcg ccaccgcttg ccagcagtgc
    25261 accaagcgcg ccaccaccta ccgccgcccc cgccagccaa ccccacggga aggcgctggt
    25321 ctgagcaacg gtatcaagaa atggcgcaat gctttcaatc gcagtggtct gggcagtcag
    25381 ttctacgggg gctaatccac ccgcagcagt atcggcaaag gtgatatggg ttagctgttg
    25441 accatcggca aacaccagtt ccgattgttc agctgtattc gccgcttgca ggaaataacc
    25501 gttgcagcgg atcaccgtgc cgtcccgcat atagatcagc aggtcatttc cttcacggac
    25561 gtaacgcgca acggcagagg cctgagcatg aacctgcacc acggaaggct cataaataac
    25621 aaccgtgacg tcgccaggat taatcagcgt ttgtttgacg gatgttttac gcgaaataac
    25681 atcaataatc aggctcataa tggcattcct gataaatggc gcacatcctt agcgagtcca
    25741 tttatctaaa taagacaaag gtaatcactt aacaacggcc cactattaat aatggcagga
    25801 ttatttcaag tcgggtaagt cgttgtataa gccctgttta attcccgcta atggaataag
    25861 gttattcacc gcagcggcat aatttacggc ggcgacccag ccatcataat tggcatttat
    25921 ttcagccgat tgcgcctgaa agacatcttg ttcgacggtt aatagatcat tcagactgcg
    25981 tttacccaat ttatattcat tttggtacac atctcgcgtt ttgtgcgcac tttcggattg
    26041 cgctaaaccg gcttcttcac gaccccgtgc gccggtccag ttcgcatatg ccacagacgc
    26101 tcgttgcagc acatccagtt ttgcctgttc gacctgcgag gcagagattt tttgttgccc
    26161 ttcggcctgt tgcacctggg cagaaaccgc gccgccctga tacagcggcg cgttaacgtt
    26221 cagttgtagc tgatcatccc aatacgagcg gtcgctggtc tggtagcgcg ttttaccccc
    26281 ctgaatactg agcgttggcc agtattgcgc tttcgttttt tccacgccgt actgtgctga
    26341 ctggcgtaag ttttctgccg ccagcaccag cgggatggac tggtaatcaa tattcttcag
    26401 cgataccggc tgctcggcta attcagcagg tggcgcggct atcgcctccg gctgtacgcc
    26461 agtgagcacc gccagttgcg ctttggcgct cgccatctgc gcctgatatt gctccagcgt
    26521 tgagcgcatt ccggcaatac gcgtctgggc ctgtaattca tccgacgatg agttcaggcc
    26581 tgcgttagcg cgcaatgccg ccatgttgta gacgttttcc agcgagtgaa tattgcgttg
    26641 agccgcatcg cacaaagcct gataacgact aacttccatg taggcagtcg ccgttttctc
    26701 tgcaacatcg gttaaggttg ccatcaattt aaagcggtag ctgtcacggg cggcagtttg
    26761 cagattgata tcgttgttag ttttaccaaa gtcatacacc agttgtgtca gggttatgcc
    26821 atacgaaacg ttgttatcaa gcctgccgct ggagtccgtt tgtcgggacg gccccgcgtt
    26881 gccggttaat ccaacgtgcg gataccaggc acttttggct tcatcaattt gcgcttcgcc
    26941 aatacctatc tgcgccgcct gttgagtgac accgggattt cgcgcaaagg cgcgtaatat
    27001 actttcctgt aaagataatt gggcaactgg cgttaccgct ggggcattcg cccattgtgg
    27061 cagcggtgca gcaattgcag cttctcccat caccataaaa cttataaaaa aaataagtcc
    27121 attattttta cggtttttaa actggaatcg cgccatagat aagtcacttc cttccaggtc
    27181 atta
//
LOCUS       DBFIFS010000042        26068 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.042, whole genome shotgun sequence.
ACCESSION   DBFIFS010000042 DBFIFS010000000
VERSION     DBFIFS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.042"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="N7A07_001653"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="N7A07_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU2476333.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="N7A07_001654"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="N7A07_001654"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU2476334.1"
                     /translation="MKGDVKTINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="N7A07_001655"
     CDS             complement(938..1405)
                     /locus_tag="N7A07_001655"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU2476335.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="N7A07_001656"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="N7A07_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU2476336.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="N7A07_001657"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="N7A07_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU2476337.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="N7A07_001658"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="N7A07_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU2476338.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="N7A07_001659"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="N7A07_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU2476339.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="N7A07_001660"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="N7A07_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU2476340.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="N7A07_001661"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="N7A07_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU2476341.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="N7A07_001662"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="N7A07_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU2476342.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="N7A07_001663"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="N7A07_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU2476343.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="N7A07_001664"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="N7A07_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU2476344.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="N7A07_001665"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="N7A07_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU2476345.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="N7A07_001666"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="N7A07_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU2476346.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="N7A07_001667"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="N7A07_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU2476347.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="N7A07_001668"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="N7A07_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU2476348.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="N7A07_001669"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="N7A07_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU2476349.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="N7A07_001670"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="N7A07_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU2476350.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="N7A07_001671"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="N7A07_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU2476351.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="N7A07_001672"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="N7A07_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU2476352.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="N7A07_001673"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="N7A07_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU2476353.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="N7A07_001674"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="N7A07_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU2476354.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="N7A07_001675"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="N7A07_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU2476355.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="N7A07_001676"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="N7A07_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU2476356.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="N7A07_001677"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="N7A07_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU2476357.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="N7A07_001678"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="N7A07_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU2476358.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="N7A07_001679"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="N7A07_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU2476359.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="N7A07_001680"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="N7A07_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU2476360.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="N7A07_001681"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="N7A07_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU2476361.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="N7A07_001682"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="N7A07_001682"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU2476362.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="N7A07_001683"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="N7A07_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU2476363.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="N7A07_001684"
     CDS             15545..15913
                     /locus_tag="N7A07_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU2476364.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="N7A07_001685"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="N7A07_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU2476365.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="N7A07_001686"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="N7A07_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU2476366.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="N7A07_001687"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="N7A07_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU2476367.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="N7A07_001688"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="N7A07_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU2476368.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="N7A07_001689"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="N7A07_001689"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU2476369.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="N7A07_001690"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="N7A07_001690"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU2476370.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="N7A07_001691"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="N7A07_001691"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU2476371.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="N7A07_001692"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="N7A07_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU2476372.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGRP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="N7A07_001693"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="N7A07_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU2476373.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="N7A07_001694"
     CDS             23092..23634
                     /locus_tag="N7A07_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU2476374.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="N7A07_001695"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="N7A07_001695"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU2476375.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="N7A07_001696"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="N7A07_001696"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU2476376.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="N7A07_001697"
     CDS             25031..25288
                     /locus_tag="N7A07_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU2476377.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="N7A07_001698"
     CDS             complement(25264..25818)
                     /locus_tag="N7A07_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU2476378.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6688 a         6178 c         6763 g         6439 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaaccataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacactggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc gacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DBFIFS010000043        25313 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.043, whole genome shotgun sequence.
ACCESSION   DBFIFS010000043 DBFIFS010000000
VERSION     DBFIFS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.043"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="N7A07_001699"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="N7A07_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGU2476379.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQA"
     gene            complement(1899..2636)
                     /locus_tag="N7A07_001700"
     CDS             complement(1899..2636)
                     /locus_tag="N7A07_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476380.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPKKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHKGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="N7A07_001701"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="N7A07_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU2476381.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3602..4105)
                     /locus_tag="N7A07_001702"
     CDS             complement(3602..4105)
                     /locus_tag="N7A07_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HGU2476382.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            complement(4120..6942)
                     /gene="traG"
                     /locus_tag="N7A07_001703"
     CDS             complement(4120..6942)
                     /gene="traG"
                     /locus_tag="N7A07_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HGU2476383.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTRGSAGMY
                     GDAHAEWGVKPKILGVGGGVGVRGGGRASIDWSDEDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQNHHKTEALSQYNKYNEEKSG
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            complement(6939..8312)
                     /gene="traH"
                     /locus_tag="N7A07_001704"
     CDS             complement(6939..8312)
                     /gene="traH"
                     /locus_tag="N7A07_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HGU2476384.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     SGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="N7A07_001705"
     CDS             complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="N7A07_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HGU2476385.1"
                     /translation="MTANKKNQEFKIRKIRRNIEYSFRDSDRYFDLFIVFLVVGIVLW
                     AVMHVIFDVCIDSWMADPKLLNFQYMWNVLMKVIPFTLWALAAGFLVTFFLSPMCELI
                     FGNIMIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8959)
                     /gene="trbJ"
                     /locus_tag="N7A07_001706"
     CDS             complement(8645..8959)
                     /gene="trbJ"
                     /locus_tag="N7A07_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HGU2476386.1"
                     /translation="MRNKQVVLLIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESSVISEETYNDSKQKESGIQNQ
                     KN"
     gene            complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="N7A07_001707"
     CDS             complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="N7A07_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HGU2476387.1"
                     /translation="MSLIKSLLFTLLLAAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYRGKKGAK"
     gene            complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="N7A07_001708"
     CDS             complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="N7A07_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HGU2476388.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            complement(9884..10231)
                     /gene="trbA"
                     /locus_tag="N7A07_001709"
     CDS             complement(9884..10231)
                     /gene="trbA"
                     /locus_tag="N7A07_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HGU2476389.1"
                     /translation="MSEDCLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(10247..10990)
                     /gene="traF"
                     /locus_tag="N7A07_001710"
     CDS             complement(10247..10990)
                     /gene="traF"
                     /locus_tag="N7A07_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HGU2476390.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAV
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(10983..11240)
                     /locus_tag="N7A07_001711"
     CDS             complement(10983..11240)
                     /locus_tag="N7A07_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HGU2476391.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTDLQKSDERKSDERNEDE"
     gene            complement(11267..13075)
                     /gene="traN"
                     /locus_tag="N7A07_001712"
     CDS             complement(11267..13075)
                     /gene="traN"
                     /locus_tag="N7A07_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HGU2476392.1"
                     /translation="MKRILPLILALVAGMALADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDSGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13072..13710)
                     /gene="trbC"
                     /locus_tag="N7A07_001713"
     CDS             complement(13072..13710)
                     /gene="trbC"
                     /locus_tag="N7A07_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HGU2476393.1"
                     /translation="MNWKIFALFALTAGMHITVHASDNVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPTLVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13738..14190)
                     /locus_tag="N7A07_001714"
     CDS             complement(13738..14190)
                     /locus_tag="N7A07_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476394.1"
                     /translation="MSNNIKYGSRILDMGVLLEMKALVNANGGVSRCIMECRQLACEV
                     RHSQQLLDKLSCIDDWLCRFSSIMYHRFDKEQIQWREDVDRNYPREKVYIFSPDDVSV
                     HNNDIKDSANNTSGITVSVTASKIFAGQKEFDAFLKLAEKLRENPTGN"
     gene            complement(14220..14681)
                     /locus_tag="N7A07_001715"
     CDS             complement(14220..14681)
                     /locus_tag="N7A07_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2476395.1"
                     /translation="MYKQATELMLNFKDRILIKGEEDTGKSTLLTEIRISDSDSRYYN
                     FKTLNSAGYNQLCDENIDDFDFLNTPEKTLILDGVRLCEKKMTSKVIRLIKQARKYHK
                     RLVVVADSCESEFIELLFDGVIALSFNSDRERSCNVYTPSRCRNTDNIYAR"
     gene            complement(14707..15699)
                     /gene="traU"
                     /locus_tag="N7A07_001716"
     CDS             complement(14707..15699)
                     /gene="traU"
                     /locus_tag="N7A07_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HGU2476396.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15696..16328)
                     /gene="traW"
                     /locus_tag="N7A07_001717"
     CDS             complement(15696..16328)
                     /gene="traW"
                     /locus_tag="N7A07_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HGU2476397.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16325..16711)
                     /gene="trbI"
                     /locus_tag="N7A07_001718"
     CDS             complement(16325..16711)
                     /gene="trbI"
                     /locus_tag="N7A07_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HGU2476398.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16708..19335)
                     /gene="traC"
                     /locus_tag="N7A07_001719"
     CDS             complement(16708..19335)
                     /gene="traC"
                     /locus_tag="N7A07_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HGU2476399.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(19495..19716)
                     /gene="traR"
                     /locus_tag="N7A07_001720"
     CDS             complement(19495..19716)
                     /gene="traR"
                     /locus_tag="N7A07_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HGU2476400.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(19851..20366)
                     /gene="traV"
                     /locus_tag="N7A07_001721"
     CDS             complement(19851..20366)
                     /gene="traV"
                     /locus_tag="N7A07_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HGU2476401.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(20363..20614)
                     /gene="trbG"
                     /locus_tag="N7A07_001722"
     CDS             complement(20363..20614)
                     /gene="trbG"
                     /locus_tag="N7A07_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HGU2476402.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            complement(20611..20928)
                     /locus_tag="N7A07_001723"
     CDS             complement(20611..20928)
                     /locus_tag="N7A07_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HGU2476403.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCTTRIVLPAPKEFS
                     SESLPHNFNMAAVGVMKEGMSMRLFSSPGHIVVAGQSGGGKSVPVKELTDRIIQKYKE
                     PIR"
     gene            complement(20925..21497)
                     /gene="traP"
                     /locus_tag="N7A07_001724"
     CDS             complement(20925..21497)
                     /gene="traP"
                     /locus_tag="N7A07_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HGU2476404.1"
                     /translation="MANNMSSRQAGHAARYVVARVLRGLFWCLKYAVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPVRPGKMKCVRVATADERLKEV
                     LAEDTSLPGKIRECHVYFPDDRTIRNNGEK"
     gene            complement(21487..22914)
                     /gene="traB"
                     /locus_tag="N7A07_001725"
     CDS             complement(21487..22914)
                     /gene="traB"
                     /locus_tag="N7A07_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HGU2476405.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(22914..23642)
                     /gene="traK"
                     /locus_tag="N7A07_001726"
     CDS             complement(22914..23642)
                     /gene="traK"
                     /locus_tag="N7A07_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HGU2476406.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(23629..24195)
                     /gene="traE"
                     /locus_tag="N7A07_001727"
     CDS             complement(23629..24195)
                     /gene="traE"
                     /locus_tag="N7A07_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGU2476407.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILRRENGVTWLDNFGETDDEKK"
     gene            complement(24217..24528)
                     /gene="traL"
                     /locus_tag="N7A07_001728"
     CDS             complement(24217..24528)
                     /gene="traL"
                     /locus_tag="N7A07_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGU2476408.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITK
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(24533..24895)
                     /gene="traA"
                     /locus_tag="N7A07_001729"
     CDS             complement(24533..24895)
                     /gene="traA"
                     /locus_tag="N7A07_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGU2476409.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSQDLMASGNSTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            complement(24929..25156)
                     /gene="traY"
                     /locus_tag="N7A07_001730"
     CDS             complement(24929..25156)
                     /gene="traY"
                     /locus_tag="N7A07_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HGU2476410.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEESESTFKEL"
BASE COUNT         6097 a         6940 c         5900 g         6376 t
ORIGIN      
        1 agcctgagtc acgccttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gggagagttt
      181 tactgccgga tacggtccgg gcagggtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catatcctta ccggtcgata ccccgtcacg
      301 taccggatca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctgggagaa cggaaaaagg cacgggtgtt
      421 catgacgtca aacagcgtgg cagccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 aggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg gatggtaaaa gactcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgacc cctgccagaa
     1021 cgggtcctct tttgtaccca tagggatcag agtatttgcg gtattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat
     1141 tttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcgtagtt ggccagacga cggataacct ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg aatccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtgatcagg caaataacca gggccacgac
     1501 agacgccaga acgaatgcgg accatagctg ctcaccgcac cagatcatat atttatcatg
     1561 cagtacctgg gcagcgttca tccggaaggt tttgccgtaa tactgaatct catatactgg
     1621 ctgggacctg attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc
     1681 gttcacaaac gtctgccagc ttatttttac ccataaaatc agaccaacga gtatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctt tatgccggtg gtgatacccg cctgcagccc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacacg ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttttgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt ttccagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc
     2581 ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 ccagcctcct gacgaggttc agagaatatt tgcgattgat ttggccaact ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac ctgatgtgcc
     3001 ctgacgcagg gcggcaacat tgtccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccggca gcaaggccca caccgagtgt ggcaccggca gaactggaat tatatccggt
     3181 gataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagattcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctttatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcaattttt ttgttttcat caaattacct ttattacaaa ccacagtttt
     3601 cttatcgcct gtcatcatag tcctgccaac aagtcccaaa agtatttcct gctgaatcaa
     3661 atcctccatg catagctaat cctgttgccg gattagtacg ataaaaatcc acataatcaa
     3721 aattcacatc atcaaaatcc agatgcaaat cttcattagt ttgttcattt acctgagatt
     3781 gcatccattc tcttacactt acacaaccac taattcgcct taattcgtat agtgtaccaa
     3841 tgataaaaac catcaggctt aataacggag agagtaagga agatactggc atcagtatat
     3901 accaaacgcc tttactgaat ttaaactcat ccgacacatg ccgttttttt cgtatttgag
     3961 aaaataacca tataaacaat ccctgccata gtacaataaa gcacaggata aaaactgttt
     4021 cggcactccc ccaatcagcc agataacagg caatgaactg tatcaccgtc accagagcaa
     4081 tatgagctaa atttttcatt atcatctccc catacaatat tattgcttgt gcttatcctg
     4141 atactccttt gctcttttca tcagttcctg tggactgtca gccccgggca ttctttcctg
     4201 acccgatttt tcttcattat atttattata ctgagataat gcctctgttt tatggtgatt
     4261 ttgtagttca gaatactgtc ttcctacaat attttcttcg ccacgtatgc tgctctgagt
     4321 atctccgata ttgcctcggt attctgtgac catattatca acctgatgtt ttacatcatt
     4381 acggatatta ctgtcctgcg ttctttgctc aataatggcc tgatgcccct gatgatcagc
     4441 aataatatcc tggctgccac cccccgaagg tacgctctcc attcctttac cgatgtcccc
     4501 acgggattca cgccaggcat tatcaacacc aggctgcacc tgttcctgaa caaaagacca
     4561 cgccatagcc cgacggcgtt ctgcaatctc cggtgaactg gtgtttgtca gaatagcttc
     4621 ggcatcctgc ggcgcgtgtt tcatcacata ctgtgcaaac tgttgcgaca ggtcttcact
     4681 catctgccca ctcatgcttt cagtacggga tgccatttca gcatattcat ggctgcgggt
     4741 catattcgtc gtgtactggt cgtaactctg ttttgctgag ttcagggctg cagacagctg
     4801 gtccacacgg gaatcggcat tattgtccgt atggctgccg gattcactga ccttgcgact
     4861 ggtaaagtaa tcgctggcct ctttaaagtc ttgtgtagcc ctggcatcga tatcatggcg
     4921 agcatcatgg ctggcccgtg aaccactgct ggcctgatgc gcatcctcat cactccagtc
     4981 aatacttgcc cgaccaccac ctctaacacc tacgccacca ccaacaccaa gaattttagg
     5041 tttaaccccc cattcagcat gagcatcgcc atacatcccc gcagaacctc tcgtacttct
     5101 tgatgccagt tctcttgtcg cctgctcatt actgatatta tgcgccttcg catagctttc
     5161 cactgcgctg cgcatacggc tggccatcat ggagtcctga gcactcatcg tgctgtcagc
     5221 accaccggtg acagaatcac tgctcccccg gttagaacca aactgactga gcgtgttcca
     5281 ggcactggca atactgctgc tgaacccatg cagggcactt tctgctctgt tcgatgcttc
     5341 ccgggccatt tcctgttgtg ccgccgcaat ctgacgcgtt gcattgatgc cgaccggtaa
     5401 ccgggacatc gctccgcttg catccatcac catattaccg tcacgggtct gtgtggcggt
     5461 tgcgccactg ccggtctggt acatcatctg accaaacgac gtggtgctgt tggtgctcca
     5521 gctgaagccg ttaacgttct ccgtctgcat gttgccgtag gagtaattac cgtcaaccac
     5581 actgcctgct gcactggcgg tcggactgat agcagaggaa gcgaaatggc tgtacacact
     5641 ggaaaatcct gccccgagcc ccctgaccat catccaggat aatggcggga tcatcatgga
     5701 aagatacccg gcagtggaag ccagatcgga gtatttcagc tgtatctgtg aaatttccga
     5761 gagcaccacc ggtgcaccat tctgctttgc ataaaatgtc atggcgctgt tcagaatggc
     5821 gtacaacatt ggccatgact gcaaccacat cagggcaaac acatagcctt tcaggacgga
     5881 cagcgtcagt ttgttgaaga cagccgccag taccaggagc gggaaaatac cgatggcgat
     5941 ccccgtcagg atggtctgcg tcatgggcag tgtccgcatc gccacatgac cgatggagac
     6001 atgtgccaga cgttgcttct ccatcgatga tgtggtggcc agattcacca ggctggccga
     6061 atcaccatta cgtgcggcat aactggtgat cccctccttc agcgcattga tggtgacatt
     6121 ctggcggatg atctggctgg ctgatttact tgagccatag aagtaactat aactgtcgcc
     6181 aatcagcgta ctgaacaaca ggttcggatc agggcgtcca ccaaatagct gctgggcata
     6241 ataatgccat gtttttccgc cactctgtgt gtccagattc agcttgtctt tcagcgaaac
     6301 cgacgcgtcc ttacaggtca cgaaattatt attgctgtca taaacgcccc gcagcggact
     6361 ggggcgggaa aaaatcagcg tgtaaggatc agcggaggcc atcagttcct ccagcgtgta
     6421 tttgtggttc aggtaaatat cccccatcac gcagttctgc acataatcct ggaaaagatt
     6481 ggcgatttcc ggattacggg acaaaaagtc ggtgcttttt gataccagtt ctgcgccaaa
     6541 cagcatcccc gttttgctgt aggtgacact gtccggttgc gtgaagatca tctcgtaact
     6601 ggccaccatt gcatgcccga tacgggtcgt cagtgaaagt ggcatcgcca gtccgaccgg
     6661 cacattatcc acccggtgaa ctttgaccag gtcactgtta tcaataatct gcaccgatgt
     6721 gcggacatta accagcaggc tgataagcac aaacacggcc acccagccca gcaaatccat
     6781 cacgttgtgc cgctgtaccc acatcacggc caccgcgaga acagacaatg tgagcgcaat
     6841 tttttcaatg gaatcccacg tcctggttcc cataaaggcg gcaatggcgt tcaggttatt
     6901 ccgcaaccac tcaccaccgg caatcacata aacttcattc acagcgtgct ccctccgaag
     6961 tgatagttgt tctggtaacg actgagcatg cgggcggaaa gctgctgacg catgtagctc
     7021 atctgacgat cgacaaccag cagcgcatcc tgctgtacct gcacctgtga ctgaaacgcc
     7081 gcaatctgcc gggtggcatc attcatgttg tcgttaatat gttcgataac tgcttcgtca
     7141 taatttcctg tggccaccat cgcccgggcc tgctgtatca gctcctgaat gtactgcagc
     7201 aggatgtcgt aaccgatata gtccgtcagc tggtaaatca tactgttgga aacaccgagc
     7261 atctgcgggt caaccaggta tttgaagacg gggatggtgg tactggaaat aaagcctttt
     7321 tccttgtcat ccagaggcgt gtcactgacc gccttgttct gaatgctggc cagaagttta
     7381 gtaatctgag acttcagtgc attatcccgg ctgatggtca ccggtgtgtc tgccaccact
     7441 ttcaggcatt tatcagaatc gttgcagtga taaatttttg ctgtgccgcc ttccatcata
     7501 gcccggataa ttgagcggtc agtggttctg gccgacagcg gtgtgatttc gccgttggga
     7561 ccaaacacca gtgagccggt cagcgtcatc acaaactctt tcagctcttt gttgccgtca
     7621 aacattctgt ttttggaaag cgcattccac atgatgttaa tgtttttggt cacccgctcc
     7681 ttatccttgt cgctggcttt atccctgacg ctgtcagatt tcccgccaac ggtacatccc
     7741 tgccgggaag ccgcccagtc agcaaaaata ttgctctcac cggcaatgtc ctgacagact
     7801 ttttgctggg acacctgcgt ccgggggaaa agcccgccga taatcccctg cgccgcctgg
     7861 caggaactga ggttcatact gttaatgtcg cttgccattt tctgcaggaa gtcttttgcg
     7921 gttttaattt ccggcaccgt tgtctgcagg gcaaggtcaa aaaagtaacc ggcagcattg
     7981 ctcataatct gcttaacaaa acgctgcagc tgctcgccat taataaaact gaacgaaccg
     8041 aggtaggcat cgatgccgcc acatccggca ttgatatccg gcagcgtcat ggaaatcagc
     8101 tgaacatttt tgacctgagt acgggcatac agggagccgc cgtaagcata gccggcagcc
     8161 tgcccctgcc agacgcccga ctgtgtggta ttggaggcga agcccagctt gttaaagaac
     8221 tgattcatgt cgctgttcac atccgctgac gctgcaggcg tcaccgtcag taaggcggca
     8281 caaagaataa aaagaggctt aatgcgaagc attattgctg ccctccctta atgtgttctc
     8341 ccggcgcata cggcgtttca gcaggaatat catgatattt ccgaatatta attcgcacat
     8401 tggggacaga aaaaatgtaa caagaaaccc tgctgccagc gcccataacg taaacggaat
     8461 gactttcatc agaacattcc acatatactg gaaattaagt aatttcgggt cggccatcca
     8521 tgaatcaata cagacatcga aaataacgtg cataactgcc cataaaacaa ttccgacaac
     8581 aaggaaaaca ataaacaaat caaaatatct gtcagaatcc ctgaaactat actcaatatt
     8641 ccgtctaatt tttctgattt tgaattcctg attctttttg tttgctgtca ttataagttt
     8701 cctctgatat tactgatgat tcattcagac cggtcatgcg gctgtaatat atttcagaca
     8761 ccaaaatcca tcccacaatt gcccccacaa aaaacacaac agaaacagaa aaggtaagac
     8821 acagcgaaac gactatttct acagggctgg cgggataaaa aagcccctga cggataaagg
     8881 ggatattcag gctgacgatt attcccgtcg cgatgccgga tattccggca atgagtaaca
     8941 ccacctgctt atttcgcacc tttttttcct ctgtacatct gcagaacggt atccatccgc
     9001 gccataaaac cggcggcatc cgttgccccc tgtaaaagcg gtaatgcctc aagcgtgttg
     9061 acgttgacca ggaaggtggt cggtgtggcc accgggatat tcgggaagaa ggtctgcatc
     9121 acgtccggtg gcaccggcaa tgcctccgga aaggcggtat ccccctgacc atccagggtg
     9181 tagggaaaaa cggaaaagcc gtactgctgt gccagctgtt tcagtaccgg gtcaaactgg
     9241 tgacagtaag ggcaatgccc ctgcataaac agcaccacct tccagtcggc caggttaacc
     9301 tgcctgccgt tgctgaggcg gaaccagcgg ggggctggct tttctgtcct ggctgacgtg
     9361 cctgctgccg gttgtgcagg ctgcgcggcc atgccctgcg gattccagag tcgttcaatt
     9421 tcatcccggg tggaggcctg cacggcagcg gctgccagca acagggtgaa caacagtgat
     9481 ttaatgagag acatgtccgc cctcctgctg ctctgccatc atggcctgtt gcctggcttc
     9541 cagtgcctca cgctcttccc ggctgacacg ggcaatccat gcatccagca cctggtatgc
     9601 gccgaacagc acaaggatgg ccgcaatcaa ctccgccaga aaaaaagcca tccagggttt
     9661 gctgtaaaca agacgggcga cgatatgaaa ccagacaccc agggaaaata cccacattcc
     9721 cgtaatatca agccggggta aagagaatct gcgtttactt atcatgttaa actccggtta
     9781 ttatttacat gagagtattt atgtctgtaa tattcgcgtt tgtgtgatat taatcagatc
     9841 aacctcatta ttttgtatgg tttttattac cgttttatat gacttagcct gtcataagcc
     9901 cccttataaa aaaccacatc actccggacc atactgatga caacataata cagataaata
     9961 ttcttctgaa cccgtcgaag ctcgtaacgt tcggtaatga gccaataacc attggaacaa
    10021 ccattgctgc gccctgattc ataattgcac cacacagaaa ccagagactg gcagtcagca
    10081 catataaaca tgcgatatta ataacaagga aaaccagtga gctcaggaga aacattttcc
    10141 tgtcattacg ctcagaaaag aaataaccgg caataatgac aaatattaac agaacaacac
    10201 ctgtaaacat tttcaaacaa tcttcactca taaatcaaat ccgcgattaa aaattgggtt
    10261 taaaatcttc agaaacgttc aggaattgtt ttgccaggtc gtcctgcgaa ataaaaccgt
    10321 atgataacgg acgaacactg ccctgtttcg ggtcaaccag catcatggcc gggaaatatt
    10381 tcacgccgag gcgctgcgcc tgcccctggt cagtccggga atccggcaac agcggattaa
    10441 tcacaccatc cacggaaacg ggaataacac tcagaccata ggtatccctg aagccattaa
    10501 tgacctgcgc cagctgcccg tcgatggggt cctgcccccg gtaaaaaaac atgatgccgt
    10561 agtgttcagc cagtttcgca atggcctgtc gctgctgcgc ctggtctgct gccagctggt
    10621 tccgtaccgt gccgttgtaa tggctgtact gcaggttata gtccagttcg ggatgcgcca
    10681 gcatggcctt tctggcgctc atggtgaaaa gcccggcctg ctgagtccag taattctgca
    10741 gccggaaata tttcacaaaa ttatccacgc cgggatacag aatagcttcg tacagcgccc
    10801 gctttgttgc cgtctgcagt gcggccagtt tctgcataat atccggctcc tgacgtggga
    10861 ctgcaggtac aggtttattt tctttttcct ttggatttat tttctcgtta taccactgcc
    10921 agcctgcatc tttccctgac gccggaaaaa taaagcagca gagtaacagt ggcagtaatg
    10981 ctttattcat cttcattcct ttcatcactt tttctttcat cagatttctg caggtcagtc
    11041 agggcagaaa aacaaaaata cagaataaac aggagcacac cggagtaaaa aataacaaaa
    11101 caggcagcaa gcatctcacc tgcagtggtt tcttttaccg tatcccacga ccagataatt
    11161 accggggaaa tgacaatcgc tgtaatcaaa agccggataa gaaaatcaat aaaacggttc
    11221 gacgtgaata tgactttcat cattttcccc ggttattttc cataccttac tgtcctgcct
    11281 gtttcagctg gtcagcaatc tgctctttca ctttctgcgt cagaacacca ctgtccggga
    11341 ttttctggtt attcatcagg tcttcgtaga agttagtgaa atccagtctg tcgaactgaa
    11401 ttttctgcag ctcatcaacc gtaatccccc gacagtcagg atgcttcgca ctgccaaaac
    11461 tgatacgcag ctgtccgttg cgcccctgct gctggactat ctgcgccagc ttcgaatcaa
    11521 actgacagta actgcgtttt ttctccaggc agacacccag cactttcttc gaacagaact
    11581 ctccgacact gacggtcagt ttgtttgatt ttgctttagc cagggctttt tcatcactgc
    11641 tgcatttggc cagcccgata tcctgtcccc agccgctgtc cttacagcag ttgctgtatc
    11701 cggctgcagc cttcttacaa aatttcgcct gaccggtgaa agcacggaca tccaccccgt
    11761 tcagcgccgc aacatcctta cctgctgcgg ccagtgccgc cagctgagaa acggcttcgg
    11821 caaaatcatt actctgcccg ctctgagcct tatcgcattc gccatcgaga cagaagacgt
    11881 ctcccccaca gaccatcact ttcccggatg tttttgactc acaggagtac gtggcatatt
    11941 catgcagaca ggcaccttct tcggagtaaa aggcgcactg gtgtgacacc agggtacagg
    12001 caggattgtc ggtgtaggtc tgacaggttc ccttgtctgc tgactgtgtg acatacgtgt
    12061 cccggtatgc ccagcagctc tggtacatgc tgtatgactg accgtctttc accagcgtcc
    12121 tgttcccgcc agcctccgta cattctgttt ttgtcagttt ccccagctcc ttgctgaacg
    12181 ggcagctttc agaccagaca acatacgggg tgtacgaggc tttttttacc ctgacaacca
    12241 gacgcagctt cattgccgta tgacgtttga attttgtatt cccgacccgg acggggtgag
    12301 atgacgtaaa agcaacaccg gcagtcagtg ttttctgtac tggtgtaaat gaggcatctg
    12361 ctgagtattt ggcattccag ctcagtgggg ttcccagtac tgtaattgtc ataggtacac
    12421 tcttctgcag gtaagtacga ctccagctgt aatgcgcatc gacaatttca ccggtaacgg
    12481 gagaggtaat ggagacaaca tactgaccat tcacttcacg tgcaggtagc tgactcattt
    12541 catactccag cgtccgggtt tcccatgtgg tactcccctg cagctccata cgggcagttc
    12601 gtgtgcagta ctgctccacc tgtaaatccc gctcacaggt gtaattcgtg tattcactcc
    12661 ggctaatctc ctgcgcactg cactgctgcc cggtgtttcc cacaatgctg tcagcccggt
    12721 tcaccacatc cctgccggtc tggataaacg gtgcatccgg tgaaagaatg tctttcggct
    12781 tgttcatgaa tgactctgta atggtttttc cggtttcacc ggtcgcccat tccgtggtgc
    12841 cgtcattttt caggccgcta tccccgccgg cagtcacgcc accgtaatat tttgtctcgt
    12901 ccggattcgc gttatagccg gggatactct cctgtggctt aaagccctga atactgctgc
    12961 ttccctgtcc tttgatctgg tgagcaaaat cagagccggc ccggtaatca ctgttgctgt
    13021 cagccagtgc catgccggca accagagcca gtatcagagg taaaatacgt ttcatttccc
    13081 ggaatctcct ttccctgcca gtaaatcatg agccacctgc cggcagtcac ccgtggcggc
    13141 cactttctcc agcgcctggc cgacccgcag gtttccccgg atgatgtcgt atccctgact
    13201 gcagaacacc accagtgtcg gtacgctgcg aatgccgtac tgtgaaaaca gcgtcgggtc
    13261 gatctgaaca ccatcagtcg cgccgtcttt caccagggac agcacggctt cagcggtggt
    13321 cttcaggtca ttgttcacca tgccccgcag tgtggcgggt ataccgtagt gccgggtttc
    13381 gcccagcatc cgtttcagcc cctcttcggg aatggaaaac gacacaaaat acagggcccc
    13441 ctgccggggt ttcccgtcct gcgaagcctg ctgtttacga accagctcat ccaggaaatg
    13501 gttatctgaa cgctgaaggg ggttctccag cacctgtttc tccgcccagg ccttcagctg
    13561 atggtcaggt ttttcacgca gttgtctgct taaattttcc tgctgcttca ggaactggcg
    13621 gttttcagga gtgttcacat tgtctgaggc atgaacagtg atatgcattc ctgcagtcag
    13681 ggcaaaaagc gcaaatattt tccagttcat gatatatcct gatggcatta ttacaattta
    13741 attacccgta gggttttctc tcagtttttc tgccagtttt aaaaaagcat caaactcttt
    13801 ctgacctgca aatattttac tggctgtaac cgacacggtt attcctgatg tgttattggc
    13861 agagtcttta atatcattat tatggacgga gacatcatcg ggactgaaaa tataaacttt
    13921 ttctctggga taattcctgt ccacatcctc ccgccactga atctgttctt tatcaaaacg
    13981 gtgatacatt attgaggaaa accggcacag ccagtcgtca atacatgaca gcttatccag
    14041 taattgttgc gaatgacgga cctcacaggc aagctgccgg cactccataa tgcaccgtga
    14101 cacaccacca tttgcattca caagcgcttt catctccagc agaacaccca tatcaagtat
    14161 tctggagcca tattttatat tattactcat tacaccctcc tctgtttttg tttacacatt
    14221 tacctggcat aaatattatc tgtattacga caccttgatg gcgtataaac attgcagctc
    14281 ctttctctgt cagaattgaa ggacaatgca ataacaccat caaacaacag ttcgataaac
    14341 tcagactcac atgagtctgc aaccacaacc aggcgtttat gatattttct ggcctgttta
    14401 attaacctga taacttttga tgtcattttc ttttcacaca gcctgactcc atcaaggatt
    14461 aacgtctttt caggtgtatt cagaaaatca aaatcatcaa tattttcatc acaaagctgg
    14521 ttataccccg cagagttaag agttttaaag ttgtaatacc gggaatcact atcactgatt
    14581 cttatttcag tcaggagagt tgacttgccg gtatcttctt cccccttaat cagaatgcgg
    14641 tctttaaagt tcagcattaa ttctgttgcc tgtttataca taaaaaatcc ttcagaatta
    14701 gtagttttac aggaagacac agttacgttt acgccacatc aggtagccga agtttttctt
    14761 tgtattgggc ggatttttcc cggtttccca gcgggtcaca ctgcgcccga acggatggca
    14821 ctgtccgctg tccgggtaca tattcaccat ctggtaacgc cagcgttctt tgggcaggat
    14881 tggggacgga tattcattac agacggcgtt atttttcccg atggtttcca taatcatgcc
    14941 ctgacggtgc agcttgaacg ccatgcgttc actgaccagc agggaggact gcaacggact
    15001 ggactcatta ctcacccagc cattgaacgg gtacatactg ccctgcgaac cggcacacca
    15061 gaacagaaca tcgagaggca tattaaaggc gctggcaatc gcatctgctg cgcaggctcc
    15121 ctgtgctatc ggattggcaa agatgacagc ttccgggttg agaatggtgg tcaggctgct
    15181 gtccgtccag gtggggtcga tttcagaaag ataagcgata tccaggtcac caccttccag
    15241 acagcccagc gatgtgatga tgttaagcca gtacgtcagc gggtatttgt accagtgaac
    15301 gtgatagaac gccccgttca cctgcttctc atcctttttc gccgttccct gtgctgtttt
    15361 accaaaagcc ggcaggctga agcccaggtt aaccatgcat cccggtgatc gggtgacgtc
    15421 cgtcagcgcc atcggctccc agtaaccaat ggccagcccg atacgcctga acagcggcgg
    15481 cggtgccgga caaatctgaa tgggcatcga cgggttcgcc gtgtcgggga ccttaccctg
    15541 actgactttg atactgccca gcgagagcgg gaaaatacag ctccagcaga tatccgtgat
    15601 cgggtttaca aaacgcccct cacaggcaga atccgcagac gctgcaggga catgaccggc
    15661 gagaaggaat aacatcagca gccacaggct tcgcttcatt ttctgccctc ctctgccgga
    15721 ataaattcca ccttcaggaa gcgatcgccg ggaacggcgc tcacccgtgc cgggacctga
    15781 tcaatgccga ggcgctggca gagcacaccg ttctggtcaa agtaaacacg gctgtccaga
    15841 gacttctgca tctccgggat actgccctgc acgaggataa ttttgctctc cagtgtgggc
    15901 ggcgtctggc gtttcatcca ggccacctgc gccggatcat cgccattgat gaaatacagc
    15961 gtctggttaa aaggaacata ctgcagcgga ttcatcactt caccctgacg ggcaaacacc
    16021 cgcccttcgt tatcccggat atccgcagcc agtctgacgg acgggtcaaa caaccggctg
    16081 ccgtatttct ccgtgcgtcc gataccgggc acggcagggg gccgcagact gttgcggatc
    16141 acccgctcct taaaggcatc cattttccgg cccatctcac cggactgctc cagtgccgtc
    16201 aggcgctgca tgatcaccgt cagcatgtcc ggctccttta ccggccacag atcgccccag
    16261 gtaccaagat cggcggcggc cacactctgc ccccatatca gcagggcaat cagcccccgg
    16321 catctcatgg ctccgccctc attcgccggg cgatatcctg ctggatttcc cgggtgatat
    16381 ccggagcccc ctgtaccacg gcaggcgaca ccagaatgac cgcgtggtgt ttctgctgcc
    16441 atgcctgcag gctggcttcc agtgccgtgt taaaacgcgc tgaaagggct tttgactgtg
    16501 cttctgacag ttgtttctga ctggcgctgt caaaaaacgc atccacggtc tgtttcatgt
    16561 tgaaggcggc agtcaccggt gcgttcagtc tgacaatgcc gtaactgatg gctgcgttca
    16621 gtaacaccat tgccaggcat cccggcaccg tccaccacca gtggctgcgt cgacgggctg
    16681 taacgtcagt cgttttttgc gttgtggtca tgcaacactc ctgtatttct catgttcttc
    16741 cagccaggct tccagcgaag ccatctccgg ccctgacttc ttccacgcca gctgccacac
    16801 cgcctcatga atactcagcc cctctttacg tttctgctgc acaaactcaa aatccgggcc
    16861 gtcagagctg tacatggcgc ggcttaacgg gtccacaaac aggcggtgcc aggaggaatg
    16921 gttttccacc tgcagcagga aggaactgaa ccactgatct ttggccgcac cgaacttacc
    16981 aatcatgtca cgctgcagtg gcaggaactg gtccggatac agctggttgt atttcgcgaa
    17041 ctccttcgca ctctgtttga ggataatttt gtaggaggag ttaccccatg ctgcacgggc
    17101 cgcactggag gccttgtcag agtcaaagtc gacgatgttc tgtgtgatgg tgatataggc
    17161 accggtatga cggcgggcgg tacggtagcc tttctcaata aattcaccga ccttgtgatt
    17221 tttgaagtcc agcagacgcc agccttcatc aatgacgttc agtttcttga ggttacgcgg
    17281 cgtgcggtac atccggttct caatgtaaat gataaggctg aacatcaccg caaccagcag
    17341 ggacggacgg tcttccagtc cgcccagctc cagcaccacc attttcgcat catcccgcag
    17401 agacggctca tcggagttga aatactggcc ataagtgccg ttagcagtgt actggtcaag
    17461 cagcacaatc atttcgtcca gacggctgcg gatggtcggt gactcggcat actgctcgct
    17521 gtcactggcg tttttcagga aatccaccac gtcatcaata cgtgccctgt tctctttggc
    17581 cagccatgat gccctgactg cctgcagcag cagaccttca tgcacttcat ccaggttacc
    17641 gttggggctg gccatcaccg acagctggtc acggacacgc tccgctgact ggtcaatatc
    17701 ggtgatgttc gcaaacgggt taaagcgcag ggtttcaccg tccagatata cgccccccat
    17761 gttctcacac agggacttgt agccatcccc catgtcgaac accacggcaa agcccccgga
    17821 gtccagcacg ctgcggataa gtggctgtat cagcccggtt ttaccggccc cggaggtgcc
    17881 acagaccgcc atgttatagt tggtgttatt catcccccgg aagaaaatat cgataaacgc
    17941 cagctggtta cggtaggtgg gtgccagcag acctgccggt gtcagggggt tatccgccac
    18001 taacggcatc aggttggcca cattaaagct ctctgcgcgc tgtaccactc cggcctcttt
    18061 cagctgttta aacaaccctt tcccggccat aaagggcaga caggtcagga aattgcgcat
    18121 gtggttaaag cgcggtgaaa tcagctcaaa accgttttta cggaagctgt tcaggatgtc
    18181 ctgctccact tccagtgccg tttcattatt atccttgcag aaggctgtga tgttgaggaa
    18241 gtaggacacc acggaggact gaccggagcc cagccgctga cgcagttccc cccactcctt
    18301 cgcctctttc tccacggacg gaaaccattt ggcataggag gtcttcgact ttttctccag
    18361 gtccatgtac ttcaggttgg cttcgctgtg ggttttcacc tggtcttcca ccaccagagt
    18421 cagcgtcagg atgaacggac aggagatgga cagttccggg ttcagcaggt tgctgtagtt
    18481 gtcggccatg ttccacagga aggcgatttc cgggttacgg gccagatgga aattcaggat
    18541 gcgggccgtg ctgttcctgc cgttctcacg caggcccagc gtcaggtaat cagcccggac
    18601 tttcagatca aaactgtcct ccacacactg ataattcaga tcagaatacg ggtccagctg
    18661 acgtcttttc gggtacaggg aatccgggtt atggttaatc atctccccga caatatcgat
    18721 aaaggcctgt gcatccaccg tctgtgtggt gatactggcc ccctgtaacg acgcccggat
    18781 gattttcacc aggttttcca tttccagaat gtcggcccgg ctttttttct tcgagggaga
    18841 acagtacgag ataaacaccc ggtaatggcg cagggtcagg ggcagattca ttccctccgg
    18901 cagcggaaac tgtgtcgctg ccgctttcat ataataggcc cgggtaatgg cgttaaaccg
    18961 ttcggcctgt tcgccagacc aggagaactc acgcagcccg tattcaatcc tgtcaccaac
    19021 cagctgactg gacatcagat ggatacacaa cggaataccg cgcggcagtt tggtgcgcag
    19081 catatgatcg agggcctcca caatggactc attcgcccca ttgatgggga tggcttccag
    19141 cataaagccc atcgtggtgt cattcatgaa cagaccggat tcctggttgt aatcacggta
    19201 cggcagcaga cggctgaact gcgggaagct catttcgccc agaacttcat tggcctttgc
    19261 ggattcgtca ggcagtttca gtgctgtgac gagggagtta accgcctgag tgacggcctc
    19321 aagtgggtta ttcacatgaa ttccttagtt gaaaaataaa gcgagaaatg cggtagccat
    19381 gaagggaata cctgtaatta acgcgccatt tcccggcgca tatacaaaga tttctgtttt
    19441 ctgaatatcc actcatgttc atccggaatt aaatcactat gttctgcgac tgacttatgc
    19501 ataatgtttt ctctgtcttt cctgatacgc ctgacattca acgcacaacg tcacaccggg
    19561 aaatattttc cgccgggctt ccggaatagg atttccgcat gcttcacaga gataaacagg
    19621 aataccatga gcatttattt tctggcgtat ccggtttatt cctgtcatgg tcagttgttc
    19681 tgtcactgaa tatgcttcat cggcttcatc actcacggaa tattcctccc gacatgttct
    19741 gacacgctac cgcccatccc ttttcacgca cagggcactc gttttacagg cagattttga
    19801 atacagaaag cccgccggca ttgccggctg atatacaact tcagggccag ttaattaata
    19861 cgtggttttc cccacgcaga cggtttaata acaaaaaaca cgctggatgg ctgatgataa
    19921 acatcctggt tatcaatata aggcgctatc cataatgctg ccgtctgctc acccgttctt
    19981 agcggacgag gtggtgttac cggcgtaact gaactgaccg gaacaaccgt tttcacttcc
    20041 cgggcagcgg taaacagcgg gcgaggtgcc agcagtttct gctccggatg tgctgtcacc
    20101 gcaggtctgc tgccggacgg agtggtcgcg gtgacggtct gcaccggcat tgtccggaag
    20161 ttcccttcag ccaggcgcgg cagtgatgcc gcaaccggct ttgcttctga ggactgctcc
    20221 agttttttgg ccttctcatt ggcctgctcc atcgtcatac aggtatcgga tgtggtggcg
    20281 ttacactcaa attccgtact ggttccggca cagccggaaa gtaataaggt ccccagcaga
    20341 ggaataaata aagaaatctg tttcattgtc cctcaccttt ctcatcgcag ttcacaaaat
    20401 cattcttcaa atccctgacc gtacaggttt ttacaatgtc aggattttca aaaaccgtat
    20461 tccgtgatga tttataaaca tatgccacaa tgcgtttccc acccgcatca ggctctggtg
    20521 cactgacttc acataacgga ggtgttattc cggactcata cagcaccgga taatttattt
    20581 ttttgacgct gccatcagaa accagcttat tcatcgaatg ggttccttat atttctgaat
    20641 aattctgtcg gttagttctt tcaccggaac agattttccg ccaccggatt gtccggcaac
    20701 cacaatatgc cccggagaag aaaataaacg catcgacatt ccctccttca tcacaccgac
    20761 agcagccata ttaaaattgt gcggcaatga ttcagaactg aattccttcg gtgcaggaag
    20821 gacgattctt gtggtgcagt tactgagaac agcatgcata aaatcatctg acacggtttt
    20881 acccggcaca gaaagggctg taataacatt aatattacgc atgttcattt ttctccgttg
    20941 tttctgattg ttctgtcatc ggggaaataa acatggcatt ccctgatttt tcccggcagg
    21001 gaagtgtctt ctgcaagaac ttccttcaga cgctcatctg ctgttgccac acggacacat
    21061 ttcatttttc cgggtcgcac cggatatttc ccggtgcctg ccgcaaacga catatacata
    21121 agtgccagcg ttgcccagaa tgcggtgacc agttgcatca gtgaccggtc agtctccctg
    21181 acataatctg cagcatcagc agcacggtaa cggcagattt tctcttctgc tcctggaaga
    21241 tcatgcagag aaggcaaaca ttctcttaca ggaaactgcc cggcaggggc tgtttgtctt
    21301 acgaagtcaa tttcctgaac cagtaatttt cctggtgtgg tgttgtcctt ccataaaaca
    21361 aacagagcca tcagggccat tgtggccagt ggcagaatga cggcgtattt cagacaccag
    21421 aacagtcctc gcagaacccg ggcgacaaca taccgggcgg catggcccgc ctgacgggac
    21481 gacatattat ttgccatcgt tgccccctcc ctggctgccg gacataaact gctgcggatt
    21541 caggttgccc agctgcttca gcatctggtc agtattaaag ccgttaagat gactttcagc
    21601 cgatggcgga acaggaaccg gactttccgg attgtcttct tccgctctgc tctgcgtgcg
    21661 ttccagcgcc atctcttcca cggttttcag ctggaagccg tcctggaaca ccacggtcac
    21721 ttcgttgccc gcaccaatcg gtatcaccgg gtgatactgt tcggcacgtt tgatgtagta
    21781 gtcactgagc gtctgtgcgg ctttcgatgc gccgccaccg atacccattt taaggacatc
    21841 gccggccccg taagcagcag tggcacccag cccgacagcc ggctgggagg cgcgctccat
    21901 tccctgaccg ataccgtcaa caaatcccgc gccccatgcc cagccgagga ttttgccgtt
    21961 acgcatcacc acttcgccct tgataccgtt tttaccccgg aagctgacat gccccttaat
    22021 cggcatatca atagttttgc cgtccttcag gcaactgata ttgcgggtgc gtacaatggc
    22081 acgctcactg gacacatccc cccaggcttc cagacccaca aaacatcccg ttgcgtcata
    22141 cgtcttgctg ttcggcattt ccaccaggcc ggtgatacgc agctgcatcg gcaccgtgga
    22201 ttcattaccg gtgacagagg cattggcatc cgccccttca atcagcatgg ctttcgcaaa
    22261 acttcctgac ggaatgtacg gcagtgatgg tccctgtttt ccctcattac gggtaaacac
    22321 cttacgctgt atccggttag gcaccggcac agactggtac gtcacctgtg gtggtggcgt
    22381 gacaccattc ccgggataaa acgccgtcgg cggtggaaca gcaacgctgc cctgcggcgg
    22441 gaaggatacg ggggtgtttc ctggctgtgg ttcgccttcc gggccgggcg gtgaggcagg
    22501 catctgcggt acaggctcac cggtcgccgt gacgggatta gcacccaggg cttttacctg
    22561 ctctgccagg gcggcattgt cctgtcccag cttttcaata cgacgctggt catcaccgcg
    22621 ctgtttgttc agaacatcca gctcacgacg gatttcctca tactgcttct gcatctgcgc
    22681 tgccgtcacc tgcatctctg tggtggcatg ctgacgcacc ttgtcatcaa aggtcgtatc
    22741 caccacaccg gtcatatccg gcacaggctc ctgttcagcc acggtttcac cgttaccgga
    22801 catgtccaca tcagacagat acagtgcccc accaatcgcg gaggctgtac cgacaaccac
    22861 aatccccagc cacaggtact gcttgcgttt cacaatggta ttgatactgg ccatcagttg
    22921 ccctccccgt cacggatgac atacacatcc atgcgcgcac ctgccagtaa ctggctggca
    22981 ggctggctga acatcacggc acgggttccc ggctgccaga agtcactttc ccggatattc
    23041 agggcagaca gcgttttatt ttccacggca aagcggacca tcttcaggtg attccccgtc
    23101 catacggcat ctgccactga agacagcccc gccggggcct gcagggtttc ctttgtcacc
    23161 gggacctgat accagcctgc gggtaatttt ccgccacgga cggcctggct gatggtcaca
    23221 agcagggatt cgtaaggcgt ggacgtttcc cacgcaccgg cttcttctcc ggttccgcgc
    23281 aggtcactga ccagctgaat ggtacgcccc gcgccttcac ggggaacggc ctgaatggaa
    23341 agattcagac cacgttctgt ttccagaatg aacgtaaagg gctttttgtt gacggtggcc
    23401 accaccacac cgccggaggc ggtctgctca ttattggtca gggcaccatc cagactgttc
    23461 acggcgataa tccggtcacc gggaacggta aacagattcg ggctggtatt gctgatggca
    23521 atactggcct gaccaccgtt caccattggc accacggtgg gggccagcgt accgtttgcg
    23581 gccatcacgc tgcaggaaaa aaacaggctg ccgaatatta ttgccgtatt attttttctc
    23641 atcgtctgtt tccccgaaat tatccagcca ggtcaccccg ttttcccgcc tcagaataag
    23701 gatgtaatgt ttaatatcag tgaaaggttt tgaatcacca atccaggttt taagcacacc
    23761 acgaatttcc acacggccat actgaggcca gacacgaaca ctggtctgga aaaaggctga
    23821 gttcacgtta tcgtttttaa tacgcttcgc ttcttcagcc agaataactt tcatctggtt
    23881 ctgtgcgccc gggcggatat attgcagaag cgcctgatgt gaggcatcga cggtttctga
    23941 tgaaacattc agacggaggg caataaatga cagcgccatc tgctgtaaat aagaggcgtc
    24001 ggcactgttc tgtgacacgg caaagggggc attaaatgcc attggcgtca cggcagtgcg
    24061 ttgctcattc tgaagacggt agttattcac cccctgaatg acgttaacag agaggctgag
    24121 aacaataagc actgacataa atataaaggc gatggccatt acacgactgg tacttaaacg
    24181 ggcaccgtgt tccatataac aatcctggta tcagttctat ttaatccact gccggaaaca
    24241 cgaatcggga acattatgaa aaataccgcg cagcagggct gttggcatat accagtaaat
    24301 caggtcacgt aaccaggaac tgccccgccc ttttttcagt tttttaatcc cgaaataaac
    24361 cagaaccgct gcaccaatac cgaacagata ttttgatttt gtgatacccc agccaataca
    24421 gattgctgcg gggatcagtt catccagtgg caggccaaac cagcggctct ggttggtcag
    24481 tgtttccggg aaacgatatt tcttaagttt attctcgtct cccgacatcg ttttatttca
    24541 ggccaacgac ggccataccc acagcaataa atacagagat gatggcaaaa ccggccagga
    24601 acttgacgtt tttggtcatc atgtacatga cagcaccgac cagaacttca gccagaacaa
    24661 cccatttaac aacactggag tccttaccga aggtcgcctt aaccgtggag ttaccacttg
    24721 ccatcaggtc ctgactaccg gcggccatcg ccagctgcgg gaagaacatc atcagaacag
    24781 cagccgggat cacagcgcga gccaggcgaa gcatattcag acgagtgaat ttggaaaaaa
    24841 acgacttctt tttgacgggc gcagaagcac cctgaacact taaaacggcg tccatatgat
    24901 tcctctgtta aatattgata gataaaaact aaagttcttt aaatgttgac tcgctctctt
    24961 cggtcacctc cctgaatatt tcttcattat aaaaatctgg ataacgttgc aaatgatcct
    25021 gcaacctcac caatgcttcg tttgttttcg ttcttccact acgctctctg gctccaagta
    25081 ataatttatt cgtttcctca tcaagggtaa gtaaaacctt tctacttggc gcagatctta
    25141 aagctccttt aatcaccctc cctcccataa ataaaagaga tttagtcaaa atattcacat
    25201 aagacgcaca caaaaataaa caacaaaata gtaaattacg ttaacaaaaa ccggcacttt
    25261 tagtgccgga aatgcttaca tttctttaca gtgaaggctg ttaatattac tag
//
LOCUS       DBFIFS010000044        24811 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.044, whole genome shotgun sequence.
ACCESSION   DBFIFS010000044 DBFIFS010000000
VERSION     DBFIFS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.044"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..740
                     /gene="yiaT"
                     /locus_tag="N7A07_001731"
     CDS             <1..740
                     /gene="yiaT"
                     /locus_tag="N7A07_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU2476411.1"
                     /translation="LINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENTK
                     AIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDKR
                     DSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVL
                     YYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSE
                     LPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(870..2840)
                     /locus_tag="N7A07_001732"
     CDS             complement(870..2840)
                     /locus_tag="N7A07_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HGU2476412.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2851..4251)
                     /locus_tag="N7A07_001733"
     CDS             complement(2851..4251)
                     /locus_tag="N7A07_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476413.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4477..5292
                     /locus_tag="N7A07_001734"
     CDS             4477..5292
                     /locus_tag="N7A07_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2476414.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5294..5434
                     /locus_tag="N7A07_001735"
                     /pseudo
     CDS             5294..5434
                     /locus_tag="N7A07_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            5536..6009
                     /gene="ysaA"
                     /locus_tag="N7A07_001736"
     CDS             5536..6009
                     /gene="ysaA"
                     /locus_tag="N7A07_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU2476415.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6161..7414)
                     /gene="avtA"
                     /locus_tag="N7A07_001737"
     CDS             complement(6161..7414)
                     /gene="avtA"
                     /locus_tag="N7A07_001737"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU2476416.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7592..9622)
                     /gene="malS"
                     /locus_tag="N7A07_001738"
     CDS             complement(7592..9622)
                     /gene="malS"
                     /locus_tag="N7A07_001738"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU2476417.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            9942..10766
                     /gene="bax"
                     /locus_tag="N7A07_001739"
     CDS             9942..10766
                     /gene="bax"
                     /locus_tag="N7A07_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU2476418.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10874..12052)
                     /gene="xylR"
                     /locus_tag="N7A07_001740"
     CDS             complement(10874..12052)
                     /gene="xylR"
                     /locus_tag="N7A07_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU2476419.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12130..13311)
                     /gene="xylH"
                     /locus_tag="N7A07_001741"
     CDS             complement(12130..13311)
                     /gene="xylH"
                     /locus_tag="N7A07_001741"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU2476420.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13289..14830)
                     /gene="xylG"
                     /locus_tag="N7A07_001742"
     CDS             complement(13289..14830)
                     /gene="xylG"
                     /locus_tag="N7A07_001742"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU2476421.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14908..15900)
                     /gene="xylF"
                     /locus_tag="N7A07_001743"
     CDS             complement(14908..15900)
                     /gene="xylF"
                     /locus_tag="N7A07_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2476422.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16266..17588
                     /gene="xylA"
                     /locus_tag="N7A07_001744"
     CDS             16266..17588
                     /gene="xylA"
                     /locus_tag="N7A07_001744"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU2476423.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17660..19114
                     /gene="xylB"
                     /locus_tag="N7A07_001745"
     CDS             17660..19114
                     /gene="xylB"
                     /locus_tag="N7A07_001745"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU2476424.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19283..19624
                     /gene="yiaB"
                     /locus_tag="N7A07_001746"
     CDS             19283..19624
                     /gene="yiaB"
                     /locus_tag="N7A07_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU2476425.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19670..20107
                     /gene="yiaA"
                     /locus_tag="N7A07_001747"
     CDS             19670..20107
                     /gene="yiaA"
                     /locus_tag="N7A07_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU2476426.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20149..21144)
                     /gene="wecH"
                     /locus_tag="N7A07_001748"
     CDS             complement(20149..21144)
                     /gene="wecH"
                     /locus_tag="N7A07_001748"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU2476427.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21319..21618
                     /gene="ysaB"
                     /locus_tag="N7A07_001749"
     CDS             21319..21618
                     /gene="ysaB"
                     /locus_tag="N7A07_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU2476428.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21713..22624
                     /gene="glyQ"
                     /locus_tag="N7A07_001750"
     CDS             21713..22624
                     /gene="glyQ"
                     /locus_tag="N7A07_001750"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU2476429.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22634..24703
                     /gene="glyS"
                     /locus_tag="N7A07_001751"
     CDS             22634..24703
                     /gene="glyS"
                     /locus_tag="N7A07_001751"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU2476430.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6129 a         6111 c         6411 g         6160 t
ORIGIN      
        1 tgttaattaa ccgcaatatt gtggcgttat ttgcgttgcc ttttatggca agcgcaactg
       61 cttctgaatt atccattggt gctggtgcgg cttataatga atcgccttat cgcggttata
      121 atgaaaatac aaaggcaatt ccgctgatta gttatgaagg tgattctttt tatgttcgtc
      181 agaccacgtt aggttttatt ctgtcgcaaa gtgaaaaaaa tgaacttagc ctgaccgcat
      241 cctggatgcc gctggaattt gaccctgccg ataatgacga ttatgccatg caacagcttg
      301 ataagcgtga tagtacggct atggcggggg ttgcctggta tcaccacgag cgttggggaa
      361 ccgtgaaagc ctctgcagct gcggacgttc tggataacag caacggctgg gtgggggagc
      421 tatcggtatt ccacaaaatg cagataggtc gtctgtcgct gacacctgcg ctgggcgttc
      481 tctattatga cgagaatttc agtgactatt actatggcat ttcagagagt gagtcccgtc
      541 gtagcggtct ggcaagttat tccgcgcagg atgcctgggt gccctatgtc agcctgacgg
      601 caaaataccc gataggagag cacgtcgtat tgatggcgag cgcaggatac agcgagctgc
      661 cggaagagat taccgacagc ccgatgattg atcgtaatga gagttttacc tttgtcaccg
      721 gcgtgagctg gcgtttttaa ttcaccggtg gatgtcggtg cggctggcct acgctctcgc
      781 accgacttgt atgcctgata agacgtgtta agcgtcgcat caggcaatgt gctccttacg
      841 ttggatgcgg cgcgagtgcc ttatccaacc tacggatggc aatgcttttc ctcattcacc
      901 cagatccgca tttcgccttc accccggttg gcccagctaa accacgggat aaaggtcaga
      961 gtttgcgtct ggcgtttggc tggcgcgctg tcgtaatgcc acagcggttg ctgctctgga
     1021 ttgctctgtt cataccggta acccggtgcc tggattaaga tcttatggct aaacaatccc
     1081 ttgccttcaa atgtcgtaaa tggcgcatcg gtgggcagcc acagattatg cagtgactcg
     1141 ccgttgtcgg ccttttccag gcaatacacc agcgggccgc gctgaatcgc cacttttccg
     1201 gcgacgtgac gcaccagcgg gttaccgtaa acgcggcgta ccggcatcgg caaagtcaga
     1261 ttcagcgtat cgccctcctg ccattcgcgg gtaatgtgca aatacccttt acgaatatcc
     1321 tgctcgacct cttccccatt caatatgatc tgcggctgtg tgcaccagtc cggcagacgt
     1381 aaagccagcg tatgacgtac cggctggggc gattcaaccg caatcgtcac ctgctcctgc
     1441 cacggatagt tcccgctaac ccgcaggcgc agcgtgccat tttctaccgg cacttccatg
     1501 ctgtttcctg cgtagatgtt gatatacaac gcatcttcac gcggcgtgta gagataatga
     1561 ccaatcgagg tcagcacgcg ggcgatattt ggcggacaac aagcgcagcc aaaccaacgc
     1621 tggcggatcg gtttaacgtg atcgtagata tggttgaatt tcagcgattt tggatgtact
     1681 tccagtggat tcacatagaa gaaatgtttg ccatccaatg ccatgccgcc gagcacagtg
     1741 ttgtacagtg cgcgctccat cacatcggca tattgactgt cgccttccat ttccagcatt
     1801 cgtcgggcga acatcatcag gccgatggaa gcacaacttt cggcgtaaac cgtgtcattc
     1861 ggcagatcgt aatcgctgct gaacgcttcg ccgctgcttt gtgagccgat gccgccggta
     1921 atatataact gacgctgggc catattgttc cacagccgca ggcagtcctg acgcttgctg
     1981 tcatcgtgac ttaaacgcgc gagatgcgcg acgccggtca tcagatagac aaaacgtacc
     2041 gcgtgaccga tggcggtttg ctgttgcgca aggggcaaat gtgcctggct gtaggctttg
     2101 tctttcacca tccacgccgg gccgtaggtg tgccagtgcg atgtctgccc gcgcttttca
     2161 tattcctggt cgtaatagtg cggttgcgca ccacgctgtt cgacaaaata gttcgtcagc
     2221 gccaggtagc gcggctcttc agtcacttca tacaggcgca ttagtgccag ttcaatttcc
     2281 gggtgaccag gataaccgtg taacttactt tcatctggac caaatacgct gtcgatatga
     2341 tcggccaggc ggcaaacgac ccccagcaag cgccgttttc ccgtggcctg gaagaaggcg
     2401 actccggctt caatcagatg acctgcgcag taaagttcat gacactccgc cagattgctc
     2461 cagcgttctt cgggtgcttt taccgtaaag taagtattga gatagccgtc ttcgcattgg
     2521 gcggaggcga tcagttcgat tacctcgtcg gcggtttttt ccagttcggc gtccggcttc
     2581 tggcacagcg accaggctac cgcttccagc catttggcga cgtcgctgtc ctgaaacacc
     2641 atcccgtaaa attcaccctc ctgaagtccg gcggcaatgc gaaagttttc aatcgcatgg
     2701 ctgggttccg cttctgggat acgatcgttc aaggcatccc actgataagg aatcaccacg
     2761 tcgcggacca gttgttggta ctgaccgagg aacggatcgc tgaccgtcag tttatgcaga
     2821 tcgacttccg aaatgttcat cttgtgctcc ttaaggctgt acatgacgct gacgcaggtc
     2881 ggtagctatg cgtgacatca taggattatt gagtttgcaa aagcggatcg cccctgccag
     2941 cagcaggtgg aatagcgcag gcagtaatgt ttccatcgcc gcgatcccgc caagcgaact
     3001 ggcggtctgg ttttccacgc caggctggta ggcgacaaaa ataaacagca ggctgatgat
     3061 cccggcgctg gaggcccagg ccagcttgat gaaaaacaga ttgaaggcga agttcatgcc
     3121 ggaagaacgt acgtgggttt tccactcgcc gtagtcatcg gcaaaggcca tcaatgagaa
     3181 gtgcagtggc aatgtgaagc caagaatcac gccattaccg aggatcaccg ccagccacag
     3241 cgtttgataa gccgggccgg agggcaggaa ccacatcaac accgccagtg cagcgaggag
     3301 caggttggtg tagtaataaa ttttgacggt atcgaaacgc cgcgttaacg ggctgacaat
     3361 caccgagccg ataatggagg cgaaggtgac catggtgaag aacagcgacg tgtagcccgt
     3421 gctgccttgt aagacgtagg taatgaagta catataccca ccgccgcgaa tgttaaagac
     3481 gttaatcagc aggaaagaca tgaccagcat cagcagcagt tggtcgttgt tccgcagccc
     3541 ggcaagatgt tcgcgcaggg taaatttccc cattgtggag agcggcaccc gttcacgaac
     3601 ccagaagaaa cagcacagga acatcaccac ggcaatggcg cacaataccc cgacgcccag
     3661 ttgatagccg cgtgcagcgt tgccctgacc gaagagagct accatccacg gtaagcctac
     3721 cgaaaccaga aatcccgcta cgccgcagag aacgaatcgc caggactggc aggagatcac
     3781 ttcattgtgg cgggtggtca tggtgttgat ccgcgcgcag tacggtacgt tgatggcggt
     3841 ataaccgact gaaaggagca ggtaagtacc gaacgcccag gcgattttta ctcccatgct
     3901 ggcatctggc acggtgaacg tcaggatgcc gataatgccg atgggaatgg caatccatag
     3961 ctgccacgga cgaaagcgcc cccatcggct ttgcgtccgg tcggcaatga cgcccatcac
     4021 cgggtcggag atggcatcaa acacgcgcag tgcaataaac agcgtgccaa ccagcgccgg
     4081 agttaatcca aaaacgtcgg tgtagaaaaa ggtgagaaaa ttcatgatca ggcaagttat
     4141 tacggtgccg cctgcgtcac ccaggccgta gcctattttt tcccgtacag ataacgcatc
     4201 gtcaatttta cctttagcga tatcagcggt agtaatcggt gtagaggtca tagatattcc
     4261 tcgtgaatag ggagcaattt ttagttttat tgaaatgacc tgattaattc tgccgtgaac
     4321 gggaatatca acaaggcgag aaaagagtaa aaaaaggtga cgattccgac ctgaatttaa
     4381 aagtgtgaaa tggatcaggt gaaattaata tttttgatta ataatcaaaa ggtaatgtgt
     4441 ttaatcatta atgaataagc gggaaattac atatcaatgc tcgaattatc catgacgtta
     4501 ccgattaaag tgcaaaatgg tggattattt atttcccgtg gcgtagggag gcattctgca
     4561 aggcggctgg agtcatggga aattatcttt gtcgaaaaag gtcgattaac gattcaggaa
     4621 gaagaacgga tatttctggt cgatgcggga gagagtttat tactctggcc gaaccgacaa
     4681 catgttgggg tggaggattt tcccgccgat ctcaaatttt actggctgca ttttgaggtt
     4741 aaagcgccgg acaacgaccc tcgctggctt actcatctta gcgtgccgca acaaacgaag
     4801 gttgtcgatc cgcaggcgct ggtggccctg tttcgccagt tcttaagcga acaggaaaag
     4861 catcaacgca ctccggcgct ggagtttatt gtgctgctca ttttgcagca gctcacccgt
     4921 gacgcgcgtc ttgatgaaca ggccgatggc gcaggcacca cccttgcctg gaaagcgcag
     4981 aaactgatcc gcacgcaata tcatcgcccg ctttctacgg cgcttctggc ccgcgagctg
     5041 tactgcaatg ccgattatct tgggcgcgtc taccggcggg tgtttcatct gacgttaaca
     5101 gaagcaatcc atcgccagcg ggtgcgcgaa gccgaaaagc tgttaattag cgaagcgcga
     5161 tcgctcacgg aagtggcggc gttgtgcggg tttaatgacg ttggctattt ccggcagatt
     5221 tttcgcaaac acaccggttt aacgccagca gcgtggaagc ggcgttacag caaggaacat
     5281 atcaattcgt agtgccgggg cgatttcgct ttcgacatca cgtctgaaac aggtggggga
     5341 gaaaaatctc ctgaaaccac tgcgtgaaac cgcgcaggct atttctaatg aactgggatt
     5401 tactgtcagg gacgaccagg gcgcgatcac ataacgcttt ggacaagtgc caaaacttta
     5461 acatttcctt cgttggatca aagcagtagg gacgcgctct ctggcactct gctgttttag
     5521 tgcaaaggag tgatcatgaa ccggtttatt attgcggatg cgacgaaatg tatcggttgc
     5581 cgtacctgtg aagtggcttg cgcagtgtcg catcaggaga atcaggattg cgctgcgttg
     5641 tcaccagacg agtttatttc ccgtattcgt gtcattaaag accacagctg gaccacggca
     5701 gtagcctgtc atcagtgtga agatgcaccg tgcgcgaatg tctgccctgt tgacgcgata
     5761 agccgcgaac atgggcatat tttcgttgaa caaacacgtt gcattggctg taaaagctgt
     5821 atgctggctt gcccgtttgg tgcgatggag gtcgtttctt cgcgcaaaaa ggcgagggcg
     5881 attaagtgcg atctgtgctg gcatcgggag acgggaccgg cctgtgttga agcctgcccg
     5941 acaaaggcgt tgcagtgcat ggatgtagag aaagtgcagc ggcatcggct acggcagcag
     6001 cctgtttgaa acgtttttcc ggcgtttctc aacgccggaa tagtgcgctt tgtttatgcc
     6061 agatgcggcg taaacgcctt atccggccca caaattcttg caaattaaat atattgcaga
     6121 aatcatgtag gcctgataag cgtagcgcat caggcaattt ttagtgactt tcagcccagg
     6181 ctctttctat ctcttccgcc agaatcttca cccccgcctc aattttctcc ggctctggta
     6241 cgtagttcat gcgcatacat tgatgcgtat gcggccacgg tttgtccagc cctgggaaga
     6301 agttgtgccc cggcaccatc agcacgccgc gtgctttcag gcgctgatag agctgctcgg
     6361 tcgtaatggg caaatcctta aaccaaagcc agaggaaaat ggctccttct ggtttatgaa
     6421 ttaggcagcg atcttccggt aaatagcggc gaatgatggc gatagtttcc tgaacacgct
     6481 ggtagtaaaa cggtttgatg actgtttcag acaggcgcag cagatcgtta cgcttaatca
     6541 tttcacacat catcgccgga ccaataccgc caggtgccag gctgataatg ccgttcatat
     6601 tggtgatggc ggtgatgatt ttttcattgg cgatgataat gccgcagcgg gagccaggca
     6661 gccccagctt ggaaagactc atgcacagca cgatattcgg attccatagc gggcgcgctt
     6721 cactgaagat gatacccggg aacgggacgc cataagcgtt atcaatcacc agcggaatgc
     6781 cgtgttgatt cgccagcgcg tcaagcttca gcaactcttc gtcagtaatc acattgcctg
     6841 ttggattcgt cggccgggag acgcaaatca tcccggtttc ttcgccaata tgcagatgct
     6901 caaaatcgac gtggtattta aactggcctt ccggcagcag ttcaatattc ggacgcgcag
     6961 agacaaacag atcttcttcc agtccggcgt cagcgtagcc aatgtattcc ggtgccagcg
     7021 ggaacagcac ttttttgacc cgaccatcgg cacggcgtcc ggcaaacaga ttaaataagt
     7081 agaaaaacgc gctctggctg ccgtttgtta gtgcaatatt ctgtgcttcg atatcccaac
     7141 ccaacttctc gcgcagcatt ccggcaagca gtgtgagtag ctccgttttc ccctgtggac
     7201 cgtcgtaatt gcacagtgca tcagtcgctt tgccactttc cagcatgtcg gtcagtagcg
     7261 tctggaagta gtcctgcatt tccgggatct gcgccggatt accgccgccg agcataatcg
     7321 cgccaggcgt gcgtaaaccg tcgttcagat cttccatcag cagcgtaatg ccggagtggc
     7381 gggtaaattt gtcaccaaaa agggagaatg tcatagcggg atatctgtcg aactctgtag
     7441 caaggaaggt aacaataacg ctacatttgc tggggtgcaa atcggtctgt tgaagagtga
     7501 gcggtgcttt atcctgcaac gctgattagg gctgacattt tatcctggtt ggcggcttcc
     7561 cgaggtactg acgaacggga agccggaaaa gtcactgttg cccagcccag atgaccagca
     7621 ctttatcgtc gccatgctca cgaacaaagc cgtagccctg cttcagcgaa agtgtcgttt
     7681 gtttgcccgc gccaattgcg ggatggcggg cgcggaactg gctgattttc tgccagtgcg
     7741 cgacgttggc ggcagattta ccgctaacat cctgccagtt catatccgaa cgtgtacctt
     7801 gcagcggatc agaacctgta ggaccgaacg gacgcgagga ttcatcgcca taaaagattt
     7861 gtaccgcgcc tggcgctaat agtaataact ctgctgcttt gtcgccccct tcacggaaca
     7921 gacgggtatc atgcgacgag aggtagctca acacgttgaa accctgcaat ttctccgcca
     7981 tttgctgcca ggtcgtatcc atctgcgcaa tacaatcgac agctttcgcc gcctgctcct
     8041 gataatcgaa attgatcatc gcatcgaagc cgtggcgata gtagtcactt tgcatcacgc
     8101 cgtggcccca ggcttcaccg gtcatccaga aaggtttgtc atctaatgct ttgtcggggt
     8161 tagctttttt ccattcgcga agcgcggcgc tggcttcggt tttcagttgc tgccaagcgg
     8221 gcaactcaac atgtttggcg gtatcgaccc gaaaaccatc aatcccatag tcgcggaccc
     8281 actgacttaa ccagtgggtt aagtaatcgc gaggggtaaa gccgtcgatg gctttagcgt
     8341 gggtatccgt tttgttttta tagaacaccg gcagaccaga agcggtagtt gattcggttt
     8401 tgatatccgg caaaaaggct agcgacatgg tgagatcgtc gaatccagga ttgtcgtaat
     8461 cgccgatatc ggtacggatc cagttttttc cccaccattt atcccagcct gttttgtcgc
     8521 tgaaattaat gtaatcgtta aagctatgcc aggtttgccc ggcggcaggt ttccagtcgc
     8581 tccagcgttc acccagcgtt tttttcactt cgtcaccaga aagatataac gcgccaaact
     8641 gatactcctg catatccgcc agcgtggcat agccggtgtg gttcatcacg acatcaaaga
     8701 gaatacgaat accgcgctga tgtgcgctat caaccagcgt ccgtagatcg gcttcgttgc
     8761 ccatattggc atcaagattc gtccagtcct gtgtgtaata accgtggtag gcataatgcg
     8821 ggaaatcgcc ttttgtaccg ccgccgaccc agccgtgaat ttgctcaaat ggggcgctta
     8881 tccataaagc attaacgccc aactgctgga ggtaatccag tttgttggtc aggccgcgta
     8941 aatcgccgcc gtgaaaagtg ccaatttccg ccataccgtc tttatgacgt ccgtaactct
     9001 ggtcattact gggatcgccg ttttcgaaac gatctgtcag cacaaagtaa accgtggcgt
     9061 tatgccagtc gaaaggggca gaggcgtcag tttctgcccg ttccagcagg agtaaaccgt
     9121 tgctggtagc agcgggttgt aacgttattt gaccgttctt cactatcgca atttgctggc
     9181 tgtaataatc ccgtacggcg gctccttccg ggaaagtggc gctgacatcc agcgtgagcg
     9241 gtaatccatc ccatttcggg cattcacgga ccaggtttgc taccggttcg gcggcgttct
     9301 ggatggaaat catcaatgtt ggcgtaccgg agcgggtgtc tatttgcagc gtatatttgc
     9361 cgtccctgaa caatcgccat tgaggcggcg tgttgctaca aggttgcagg gaaagcatct
     9421 gattgagttt tatcgcatct gcaggctgcc agcactgctg gtcaaaattt agcgtgagtg
     9481 gacgcgtacc tttgggcaac tgcgcgtggc tgacaaatgt tcccgttccc tgttcgctaa
     9541 aggcagggaa ccccggagaa gtccagctgg cggcaacggc gaagccagga aggagtgtca
     9601 gaaaacaggc ggcgagtttc atggtggatg agtccttatt gctgcgattt cagacagttt
     9661 gccagcgata agccagacaa aactcatccc tgagcggtaa gtccacagga tgagaagcaa
     9721 gggtgagcga tcgcgcgcaa aaaacggctg aattttgcga taaaccccac attttctgcg
     9781 atttagcgcc aatctgaatc gttaactcgt gacatagttt cagatttgga ctattccttg
     9841 gggtgctctt gacagctatt tttgatatga tttgagattc aactctcaaa tttgtgaaaa
     9901 atataaggtg ttggaatgat taaatccgac caggagacct gatgattttg actcccatac
     9961 gacgatatgg ggcgatgatt cttatgttac tcactctggt gttttcgagt gaggtgttag
    10021 cgaagacgca cacaacaaca gcgagtcaaa agtcccactt aactaaagct agtaataaac
    10081 aggtaagcag taaacaagag tattctcgca atagtgcaaa gagtagttca cttcctgatt
    10141 tgcgaaaata cccttccgga acatcaagga aaaaggcgtt tctccggacc gtaatgcctt
    10201 acattaccag ccaaaatgca gccattactg cagaacgtaa ctggctgatt tcaaaacagt
    10261 atcagggcca atggtcacct gctgagcgtg cgcgtctgaa agacatcgcc aaacgctaca
    10321 aggtgaagtg gtctggtaat acgcgaaaaa tcccgtggaa taccttgctt gaacgcgtag
    10381 acattatccc taccagtatg gtggcgacga tggctgcagc agaaagcggt tggggaacgt
    10441 cgaagctggc gcgcaacaac aacaatcttt tcggcatgaa atgcatgaaa ggacgttgta
    10501 ccaatgcgcc aggtaaagtg aaagggtact cacagtttag ttctgtcaaa gaatcggtga
    10561 gcgcctatgt cactaacctc aatacgcatc cggcttactc ttcgttccgt aaatcgcgtg
    10621 cgcagctgcg taaagctgat caggaagtga ctgccacagc gatgattcac aagctgaagg
    10681 gctactcgac caaagggaag agttataaca actacctgtt cgcaatgtac caggataacc
    10741 aacggttaat cgcggcgcat atgtaattgt atttcctcga gccttatccg acttgtcggt
    10801 cggataaggc ttttgatcgc atcaggtggt tgtgctgagt tacctgatgc gacgccgaca
    10861 attctcatca tcgctacaac atgacctcgc tatttacatc gcgatactct tttggcgtcg
    10921 tgtcatatgc ttttttaaaa acagagtaga aatattgcag cgatggataa ccgcacattt
    10981 gcgatatctc attgatcgac aaggtggttg aaatcagcag actgcgcgct ttctccagct
    11041 tctcagcatg aatcatggca tggatggttt cacccacctc ttctttaaaa cgcttctcaa
    11101 gattggagcg cgagatcccg accgcatcga gtacctgatc cactttaatc cctttacagg
    11161 cgtgattacg aatgtaatgc atggcctgaa taacggcggg atcggtcagc gaacggtaat
    11221 ctgttgagcg ccgttcaatg acgcgaactg gagggaccaa aatccgctgt agcggcattt
    11281 cttctttatc taataatcga tgcaacagtt ttgccgcctg atagcccatt tgccgcgcgc
    11341 cctgagcgac cgaagaaagg gcgacacgcg acagatagcg ggtcagttct tcgttatcga
    11401 tgccaatcac gcataatttt tccggtacgg gaatgtgtag atgttcacat acttgcagaa
    11461 tatgccgcgc ccgggcgtca gtaacggcaa taatcccggt ttgcggtggc agtgtttgta
    11521 gccagtctgc cagccgattt tgcgcgtgtt gccagttctc tggtgcggtt tctaacccct
    11581 gataaaccac tccgcgatac ttttcttcgg cgacaagctg acgaaatgca tattcgcgtt
    11641 cagtggccca acgtttgccg cttgattccg gaagaccata aaaagcaaag cggttaacgc
    11701 ctttctcttt taaatgcaaa aatgcgcttt caaccagcgc atagttatcg gtggcaatgt
    11761 aatgaacggg tgggtaactt tctgcaaggt gatacgagcc gccaacccca acaatgggga
    11821 cgtcgacatc agccagcgct tgctcgatct gtttgtcgtc gaagtcggca atgacgccat
    11881 ctcctaacca gtccttgatt ttatcaatgc gggcgcggaa atcttcttca atgaaaatat
    11941 cccattccga ttgtgacgcc tgtaaatatt cccctacgcc ttctactacc tgccggtcat
    12001 aggctttatt ggcattgaac agtaatgtga tgcggtgacg tttagtaaac atggttcttt
    12061 tcctgctgaa tcatgcaaaa actcaaaacc ggtaatacgt taccggcttt gagaaaattt
    12121 ttatcaaaat caagaacggc gtttggttgc ggagtccatc catactgcca gcaacagaat
    12181 cgcaccttta acgatatact gccagaaggt cggtacatcc atcatactca taccgttatc
    12241 cagtgaagcc atgatgaatg cccccattac cgctccagcc acgctcccga cgccgcctgc
    12301 cagactggtg ccgccaatca cgcatgctgc aattgcgtcc agctcggcga tattccccgc
    12361 agaaggtgaa ccagcgccaa gtcgagaact gaggattaat ccggcgatgg ctaccattaa
    12421 tccgttaata gcgaacacgg caagtttggt gcgttcaacg ttaattccgg agagacgtgc
    12481 tgcttccaga ttgccgccga tggcataaat gcgtcgtcca aatgctgtcc gcgttgccat
    12541 aaacattccg ccgagtaaca gcaacgtcag cagcagaaca ggagtaggaa cgccacggta
    12601 atcattcaac agccagattg cgcctaatac gatgatagcg gttaaagcct ggcgaccgac
    12661 tactgcggta gaggctggag actgcaaacc caaagcctga cggcgcattc ttccgcgcca
    12721 ttgccagcca acaaaagcca ttaagccaag cgcgccaatg atgaagccgg tgctggcggg
    12781 gagatagctt tgcccaattt gtgacatcgc ggcgctggtg ggggatacag tcgtgccgtt
    12841 ggtgatgcca atgagtatgc cgcgaaatgc caacatgccc gcgagagtga caataaatga
    12901 agggactttg cggtacgcga cccaccatcc gttccaggca ccgagaagca gtcccagaac
    12961 caacgtcaca atgatggtaa gtggcaaagg ccagcctagc cagacgtcac aaatcgccgc
    13021 gacgccacct aatagcccca tcattgagcc gacggaaagg tcgatttcag cagaaattat
    13081 gacgaacacc attcctaccg cgaggatgcc ggtaatcgcg gtctggcgta acaggttgga
    13141 gacgttacgg gcgcttaagt aggcaccatc ggtggtccag gtaaagaaca gcatgattgc
    13201 gatgatagct gcaatcatca cgaagacctg caaattcagt gatttcagcc cggagaagcc
    13261 accggatgtc ggtacggcca atttcacttc agacggattg cttttcgaca tgatgttcgc
    13321 tcctcaatgc ggcttccatc acctgctcct gagtcaggtt atgatttatc aggttggctt
    13381 ttagtttccc ttcatgcatt actagtacac gatcgctaag gccgagcact tcaggtaatt
    13441 cagaagagat gacaataacg gcaataccct gctggacgag ttggttaatt aatttgtaga
    13501 tctcgtattt cgcgccaata tcgatacccc tggtgggttc atcaagaatg agaatgcgcg
    13561 ggttaagtaa cagacagcga gcgaggatcg ctttttgctg attgccgccg ctcaaacgtc
    13621 caatagcaag gtcgggggac gacgttttaa ctttgagttg ctggattgat tccagaatac
    13681 atttttgctc tgccgcgtca tcaagctggc taatgccacc ggtaaattta ttgagtgcgg
    13741 cgagggtaat atttttacca accgccatta ccggaacgat gccgtcgcgc tttctgtctt
    13801 cggggaccat cgcaatcccc tgggcgatgg cttgctgaca gttacgaata tctacctgtt
    13861 tgccatcaat ataaattttt ccttcccatt gtccgggcca cacaccaaac aggcactgaa
    13921 tggtctcggt acgtccggca ccaacgagtc cggcaatacc cagtatttcg ccacgtttca
    13981 gggaaaacga gacatcatta actcgtttaa tatgacgatt aaccggatgc catgccgtca
    14041 gatgttcaat acgtaatatt tcatctccgg tggtatgtgg ttcattaggg taaagcgcgg
    14101 ttaactctcg cccgaccatc atggtgataa tatcgtcttc actcattccg gcagcatcac
    14161 gcgtaccaat gtgctgtccg tcgcgaataa cgcaaatcgt atcggaaatc gctttgactt
    14221 cgttgagttt gtgcgaaata taaatacagg cgataccgtg ctgttgtaga tcgcgaataa
    14281 tatccagtaa aaccgacgtt tcctgctcag ttaatgaggc tgtcggttca tcgagaatta
    14341 acaagcgcac ctgtttatta agtgccttgg caatttcaac cagttgttgt tgcccaagcc
    14401 ctaaatcgcc aacgcgggta tcaggtgaaa tggataaact gacctgtgcg agcagcttct
    14461 gacagcgtag cgtcatcagg tcataatcca taatgccatt gtgggttatt tcgttaccca
    14521 ggaagatatt ttccagcacg gtcaattctt tcaccagggc caattcctgg tgaatgatgg
    14581 cgataccttt gcgttcggta tcgcggatgt gactcgcctg aatctcttct cccgcaaaaa
    14641 taatttcgcc ttcgtaggag ccatggggat aaataccaca cagcactttc atcagcgttg
    14701 atttaccaga cccattttcc ccacagagtg agacgatttc gccagcattc aaccgcaagc
    14761 tgacgttatc aatcgccttc acactgccga aggttttggt aatgttcttc atttcaagta
    14821 gataaggcat agcgactcca cctaagccaa ttcattcacg cggcatggag agaaatcacg
    14881 cccccgctcc gcgctggggc gtaacgctta cagctcgctc tctttgtgga atccgtcttt
    14941 aattaccgta tctttgatgt tgtttttatt cacatcgatc ggtgtcagga ggcgggaggg
    15001 gacatctttc aggccattat ttagtgaagt atctgctttt ggctcctgac cattgcccaa
    15061 ttcaacggca atttctgcgg cagtatttgc cagcaatgta ataggtttat ataccgtcat
    15121 agtttgcgta ccggcagcaa tacgtttaat acctgcgaga tccgcatcct ggccagaaat
    15181 tgctactttc cctgataaac cttgcgcgct taatgcctga attgccccac ctgcggtggc
    15241 atcgtttgag gcaactacag catcaatttt gttattattg gcggttagcg cgttttccat
    15301 aattttcaat gcgttttccg gtaaccagcc atcaacccat tggtcaccaa cgactttaat
    15361 ttttccggaa tcaacgtaag gttgtaacac tttcatttgt ccggcgcgga acagcttagc
    15421 gttgttatct accggcgagc cgcccatcag gaagtaatta ccttgcggaa caatatcgac
    15481 cagggctttt gcctgcagtt caccgacttt ttcgttatcg aaagaaatat aaaaatcgat
    15541 atccgcatcg ttaatcatac ggtcgtaagc taatacttta atgccttctt gtttggcttc
    15601 ttttacaacg ttacttaata cctgaccgtt atacggaata atgacaagaa catcgacacc
    15661 ccggtttatc atgttttcaa tctgcgacat ttgtgtttct tcattgccat ttgcagactg
    15721 tacaaatact ttcgcgccga gagattctgc ctttttcaca aagatatctc gatctttttg
    15781 ccagcgttca agacggagat catcaatcgc catacctatt ttgacttctt tggcgtgtgc
    15841 agcaacgttg gtaagcagga gtgaggtgca aagggtgagt agaatgttct ttattttcat
    15901 ggtgtagggc cttctgtagt tagaggacag ctttaataag taacaatcac cgcgataaac
    15961 gtaaccaatt tttagcaact aaacagggga aaacaattac agatttttat ctttcgatta
    16021 cgatttttgg tttatttctt gatttatgac cgagatctta cttttgttgc gcaattgtac
    16081 ttattgcatt tttctcttcg aggaattacc cagtttcatc attccatttt attttgcgag
    16141 cgagcgcaca cttgtgaatt atctcaatag cagtgtgaaa taacataatt gagcaactga
    16201 aagggagtgc ccaatattac gacatcatcc atcacccgcg gcattacctg attatggagt
    16261 tcaatatgca agcctatttt gaccagctcg atcgcgttcg ttatgaaggc tcaaaatcct
    16321 caaacccgtt agcattccgt cactacaatc ccgacgaact ggtgttgggc aagcgtatgg
    16381 aagagcactt gcgatttgcc gcctgctact ggcacacctt ctgctggaac ggggcggata
    16441 tgtttggtgt gggggcgttt aatcgtccgt ggcagcagcc tggtgaggca ctggcgttgg
    16501 cgaagcgtaa agcagatgtc gcatttgagt ttttccacaa gttacatgtg ccattttatt
    16561 gcttccacga tgtggatgtt tctcctgagg gcgcgtcgtt aaaagagtac atcaataatt
    16621 ttgcgcaaat ggttgatgtt ctggcaggca agcaagaaga gagcggcgtg aagctgctgt
    16681 ggggaaccgc taactgcttt acaaaccctc gctatggcgc gggtgcggcg acgaacccag
    16741 atccagaagt cttcagctgg gcggcaacgc aagttgttac agcgatggaa gcaacccata
    16801 aattgggtgg tgaaaactat gtcctgtggg gcggtcgtga aggttacgaa acgctgttaa
    16861 ataccgattt gcgtcaggag cgtgaacaac tgggccgctt tatgcaaatg gtggttgagc
    16921 ataaacataa aatcggcttc cagggcacgt tgcttatcga accgaaaccg caagaaccga
    16981 ctaaacatca atatgattac gatgctgcga cagtctatgg cttcctgaaa cagtttggtc
    17041 tggaaaaaga gattaaactg aacattgaag ctaaccacgc gacgctggca ggtcactctt
    17101 tccatcatga aatagccacc gccattgcgc ttggcctgtt cggttctgtc gacgccaacc
    17161 gtggcgatgc gcaactgggc tgggacaccg accagttccc gaacagtgtg gaagagaatg
    17221 cgctggtgat gtatgaaatt ctcaaagcag gcggtttcac caccggtggt ctgaacttcg
    17281 atgccaaagt acgtcgtcaa agtactgata aatatgatct gttttacggt catatcggcg
    17341 cgatggatac gatggcactg gcgctgaaaa ttgcagcgtg catgattgaa gatggcgagc
    17401 tggataaacg catcgcgcag cgttattccg gctggaatag cgaattgggc cagcaaatcc
    17461 tgaaaggcca aatgtcactg gcagatttag ccaaatatgc tcaggaacat aatttgtctc
    17521 cggtgcatca gagtggtcgc caggagcaac tggaaaatct ggtaaatcat tatctgttcg
    17581 acaaataacg gctaactgtg cagtccgttg gcccggttat cggtagcgat accgggcatt
    17641 tttttaagga acgatcgata tgtatatcgg gatagatctt ggcacctcgg gcgtaaaagt
    17701 tattttgctc aacgagcagg gtgaggtggt tgcttcgcaa acggaaaagc tgaccgtttc
    17761 gcgcccgcat ccactctggt cggaacaaga cccggaacag tggtggcagg caactgatcg
    17821 cgcaatgaaa gctctgggcg atcagcattc tctgcaggac gttaaagcat tgggtattgc
    17881 cggccagatg cacggagcaa ccttactgga tgctcaacaa cgggtattgc gccctgccat
    17941 tttgtggaac gacgggcgct gtgcgcaaga gtgcgctttg ctggaagcgc gagttccgct
    18001 atcgcgggtg attaccggca acctgatgat gcccggattt actgcgccta aattgctatg
    18061 ggttcagcgg catgagccgg agatattccg tcaaatcgac aaagtattat taccgaaaga
    18121 ttacttgcgt ctgcgtatga cgggggagtt tgccagcgat atgtctgacg cagctggcac
    18181 catgtggctg gatgtcgcaa agcgtgactg gagtgacgtc atgctgcagg cttgcgactt
    18241 atctcgtgac cagatgcccg cattatacga aggcagcgaa attactggtg ctttgttacc
    18301 tgaagttgcg aaagcgtggg gtatggcgac ggtgccagtt gtcgcaggcg gtggcgacaa
    18361 tgcagctggt gcagttggtg tgggaatggt tgatgctaat caggcaatgt tatcgctggg
    18421 gacgtcgggg gtctattttg ctgtcagcga agggttctta agcaagccag aaagcgccgt
    18481 acacagcttt tgccatgcgc taccgcagcg ttggcattta atgtctgtaa tgctgagtgc
    18541 agcgtcgtgt ctggattggg ccgcgaaatt aaccggcctg agcaatgtcc cagctttaat
    18601 cgctgcagct caacaggctg atgaaagtgc cgagccagtt tggtttctgc cttatctttc
    18661 cggcgagcgt acaccacaca ataatcccca ggcgaaaggg gttttctttg gtttgactca
    18721 tcaacatggc cccaatgaac tggcgcgagc agtgctggaa ggcgtgggtt atgcgctggc
    18781 agatggcatg gatgtcgtgc atgcctgcgg cattaaaccg caaagtgtta cgttgattgg
    18841 tggcggggcg cgtagtgagt actggcgtca gatgctggcg gatatcagcg gtcagcagct
    18901 cgattaccgt acgggggggg atgtggggcc agcactgggc gcagcaaggc tggcgcagat
    18961 tgcggcgaat ccagagaaat cgctcattga gttgttgccg caactaccgt tagaacagtc
    19021 gcatctacca gatgcgcagc gttatgccgc ttatcagcca cgacgtgaaa cattccgtcg
    19081 cctctatcag caacttctgc cattaatggc gtaaacgtta tcccctgcct gaccgggtgg
    19141 gggataattc acatctatat atctcagcaa ttaattaata tttagtatga atttattctg
    19201 aaaatcattt gttaatggca tttttcagtt ttgtctttcg ttggttactc gtaatgtatc
    19261 gctggtagat atggagatcg ttatgaaaac ctcaaaaact gtggcaaaac tattatttgt
    19321 tgtcggggcg ctggtttatc tggttgggct atggatctca tgcccattgt tgagtggaaa
    19381 aggctatttt cttggcgtgt taatgacagc aacttttggc aactatgcat atcttcgcgc
    19441 agaaaaactc gggcaactgg ataatttttt tacccatatt tgccagttag ttgcgttaat
    19501 cactatcggt ctcttgttta tcggtgtttt aaatgcacct atcaatgctt atgaaatggt
    19561 gatctacccc atcgcctttt ttgtctgctt gtttggtcaa atgcgtttgt ttcgctcggt
    19621 atgagcaaca taaagctctt acacattcag gaatgaaagg aatactgtga tggacaacaa
    19681 aatatcaacc tattcaccgg cctttagtat tgtgtcatgg atagctctcg ttggtggtat
    19741 cgttacctat ctgttagggc tatggaatgc agagatgcag ttaaatgaaa aaggatatta
    19801 ttttgccgta ctggtattag gactgttttc tgcggcgtct tatcaaaaga ccgttcggga
    19861 caagtatgaa ggcataccga ccacttccat ttattatatg acctgcctga ctgtctttat
    19921 tatctctgtt gcattactga tggtaggtct gtggaatgcg acattattac tcagcgaaaa
    19981 aggtttttat ggactggctt tcttcttaag cttgtttggt gcagtagcgg tgcagaaaaa
    20041 tattcgtgat gccggaataa acccaccaaa agaaacacag gttacccagg aagaatacag
    20101 cgaataactc acgtaagccc ggtcagtcca atgtgaccgg gcttttactt aactcactaa
    20161 tctgtttctg tcgattcgtt gtaccagcat agaaagtaac aaactcgctg ccaacgtcgc
    20221 gcaaaaaatc caaataatat ccagtattgg ccaattttta agctcaattc cccgggtgcg
    20281 cagcgcatgg ataatcaagg cgtggaatcc gtatataccc aacgaatggc gggagattaa
    20341 gccaagtccg cgaatggtac gcgtatccag cgtgttttta accagagtca atagcgcgat
    20401 tgcgcagata aaaaccatcg gcccacagta aggataccag gtatcggcaa aatttccgcg
    20461 ccactgcaat tcatataatg tcccgcgaga gataataaaa actcccgtcg caaacagtgc
    20521 ggcgctcacc cacgacagtg ctttatgctg tgtgtccatc atccctaaag cgcggcccaa
    20581 catgccatac agaatgtagt aaaaagtatc gccattgata tataagttaa ttggcagcca
    20641 ttcaaaaccg tcgattttct gcggcacggt gtttgggtta gcgatgatac caatcaccac
    20701 cattagcacc agcaacattt ttccgccgac gttcttcacc tgaatcagcg gtgaaaccag
    20761 ataaatcacc gcaatcgcga agaaaaacca taagtggtaa aacactggct tttgcagcag
    20821 gtttttcagc gctaactcca cattaatgga ggtaaacagt gcaatgtaga gcagcgcgat
    20881 tgcgctataa aaaaacagac ataagccgat acgcaagaaa tggcgcggct gggcgctgcg
    20941 ttcgccaaaa aagagatagc cggaaatcat gaaaaatagc ggcacgctga cacgagaggc
    21001 agagttcaga acattggcga tatcccaggt gacggggcta acactatgag cattggtcac
    21061 ataccaggta gtggtgtgaa tcatcaccac cattaaacac gctatccctc gcaggttatc
    21121 aatccagtaa attttgggct gcatctgtgt ctctgtatct ggttaaaaaa agtctgaccg
    21181 ataaatcatt cagttggcgc actggaataa tctgagtttt aacactacag cttatagagg
    21241 cttaaggaaa ttcgtaagat atcagccact ataccgatat aaataataag actcacctgc
    21301 aaaccagacg gtaatttaat gatgatgaac tctttctttc cggcaatggc gcttatggtg
    21361 ctagtgggtt gttctacacc gtcacccgtg cagaaagcac aacgggtaaa ggttgatcct
    21421 ctgcgttcgt tgaatatgga agcgttatgc aaggatcagg cggcaaaacg ttataacacc
    21481 ggcgagcaaa aaatcgacgt caccgccttc gaacagttcc agggaagcta tgaaatgcgc
    21541 ggttatacct tccgtaaaga gcagtttgtc tgttcttttg acgcagatgg ccattttttg
    21601 catctttcca tgcgttaagc cctgcttttt cccgtttcat actgtatatc ttccatccag
    21661 cgggtatact gatcccttcc tttaaatcca cacgtatcca gcacgaaata atatgcaaaa
    21721 gtttgatacc aggaccttcc agggcttgat cctgacctta caggattact gggctcgcca
    21781 gggctgcacc attgttcaac cattggacat ggaagtcggc gcgggaacct ctcacccaat
    21841 gacctgtctg cgcgcgctgg ggccagaacc gatggcggct gcttatgttc agccttctcg
    21901 tcgcccgacc gatggtcgct acggcgaaaa ccccaaccgt ttacagcact actatcagtt
    21961 ccaggtggtc attaagccat cgccggacaa tattcaggag ctgtaccttg gttctctgaa
    22021 agagctgggc atggacccga ctatccacga tatccgtttc gtggaagata actgggaaaa
    22081 cccgacgctg ggtgcctggg gactgggctg ggaagtgtgg ctaaacggca tggaagtgac
    22141 gcagttcact tacttccagc aggttggtgg tctggagtgt aaaccggtta ccggcgagat
    22201 cacctacggt ctggaacgtc tggccatgta cattcagggc gtagacagcg tttacgacct
    22261 ggtctggagc gacggcccgc tgggtaaaac cacctacggc gacgtgttcc atcagaacga
    22321 agtggagcag tccacttaca acttcgaata cgcggatgtg gacttcctgt tcacctgctt
    22381 tgagcagtac gagaaagaag cgcagcagct gctggcgctg gaaaatccgc tgccgctgcc
    22441 agcttacgag cgtattctga aagccgccca cagcttcaac ctgctggatg cgcgtaaagc
    22501 catctccgtc accgagcgtc agcgctacat tctgcgcatt cgcaccctga ccaaagcagt
    22561 ggcagaagca tattacgctt cccgtgaagc cctaggcttc ccgatgtgca acaaagataa
    22621 gtaagaggcg gctatgtctg agaaaacttt tctggtggaa atcggcactg aagagctgcc
    22681 accaaaagca ctgcgcagcc tggcagagtc ctttgctgcg aactttactg cggagctgga
    22741 taacgcaggc ctcgcacacg gcaccgttca atggtttgct gctccgcgtc gtctggcgct
    22801 gaaagtagct aacctggcgg aagcgcaacc ggatcgtgaa atcgaaaaac gcggcccggc
    22861 gattgcccag gcgttcgacg ctgaaggcaa accgagcaaa gcggcagaag gttgggcgcg
    22921 tggttgcggt attaccgttg accaggctga gcgtctgact accgataaag gcgaatggct
    22981 gctgtatcgc gcccatgtga agggcgaaag cactgaagca ctgctgccga atatggttgc
    23041 gacttcgctg gcgaaactgc cgattccgaa actgatgcgc tggggtgcaa gcgacgtgca
    23101 cttcgtgcgt ccggtgcaca ccgtgaccct gctgctgggc gacaaagtca ttcctgcaac
    23161 cattctgggc attcagtccg atcgcgtgat tcgcggccac cgctttatgg gcgagccgga
    23221 attcaccatc gataacgccg atcagtatcc ggaaattctg cgtgagcgtg ggaaagtcat
    23281 cgccgattac gaagaacgta aggcgaagat taaagccgat gccgaagaag cagcgcgtaa
    23341 gattggcggt aacgctgact taagcgaaag cctgctggaa gaagtggctt cgctggtgga
    23401 gtggccggtc gttctaaccg cgaaatttga agagaaattc ctcgcggtgc cgtctgaagc
    23461 actggtttac accatgaaag gtgaccagaa gtacttcccg gtgtatgcga acgacggcaa
    23521 actgctgccg aactttatct tcgttgccaa catcgaatcg aaagatccgc agcaaattat
    23581 ctctggtaac gagaaagtcg ttcgtccacg tctggctgat gccgagttct tcttcaacac
    23641 cgaccgtaaa aaacgtctgg aagataacct gccgcgcctg caaaccgtgc tgttccagca
    23701 acagctgggt acgctgcgtg acaagactga ccgcatccag gcgctggctg gctggatcgc
    23761 cgaacagatt ggcgctgacg ttaaccacgc aacccgtgcg ggcctgctgt ctaagtgtga
    23821 cctgatgacc aacatggtct tcgagttcac cgacacccaa ggcgttatgg ggatgcacta
    23881 cgcgcgtcac gatggcgaag cggaagacgt agccgtcgcg ctgaatgagc agtatcagcc
    23941 gcgctttgcc ggtgatgacc tgccgtccaa cccggtagct tgtgcgctgg cgattgctga
    24001 taagatggat actctggcgg gtatcttcgg tatcggtcag catccgaaag gcgacaaaga
    24061 cccgtttgcg ctgcgtcgtg ccgcacttgg cgtgctgcga attatcgttg agaagaacct
    24121 caaccttgat ctgcaaacgc tgaccgaaga agcggtgcgt ctgtatggcg ataagctgac
    24181 taatgccaac gtagttgatg atgttatcga ctttatgctc ggtcgcttcc gcgcctggta
    24241 tcaggacgaa ggttataccg ttgacaccat ccaggcggta ctggcgcgtc gtccgactcg
    24301 tccggctgat ttcgatgccc gcatgaaagc ggtatcgcac ttccgtaccc tggaagcagc
    24361 tgcagcactg gcggcggcga acaaacgtgt atctaacatc ctggcgaaat ctgacgaagt
    24421 gctgagcgat cgcgtgaatg cctctactct gaaagagccg gaagaaatta aactggcgat
    24481 gcaggttgtg gtgctacgtg acaagctgga gccgtacttt gcggaaggtc gttaccagga
    24541 tgcgctggtc gaactggcag agctgcgtga accagtcgat gccttcttcg ataaagtgat
    24601 ggtcatggtt gatgacaaag aattgcgtat caaccgtctg accatgctgg agaaactgcg
    24661 cgaactgttc ctgcgcgttg cggatatttc gctgttgcag taataacgcc gttattaaaa
    24721 agcctgccat ctggcaggct ttttttatcg ctaaataata cagcaacctt taataatctt
    24781 ctgctgaata aagattatct catatattaa t
//
LOCUS       DBFIFS010000045        24064 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.045, whole genome shotgun sequence.
ACCESSION   DBFIFS010000045 DBFIFS010000000
VERSION     DBFIFS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.045"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(196..561)
                     /gene="yhaH"
                     /locus_tag="N7A07_001752"
     CDS             complement(196..561)
                     /gene="yhaH"
                     /locus_tag="N7A07_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU2476431.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(853..1839)
                     /gene="yqjG"
                     /locus_tag="N7A07_001753"
     CDS             complement(853..1839)
                     /gene="yqjG"
                     /locus_tag="N7A07_001753"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU2476432.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1909..2301)
                     /gene="yqjF"
                     /locus_tag="N7A07_001754"
     CDS             complement(1909..2301)
                     /gene="yqjF"
                     /locus_tag="N7A07_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU2476433.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2487..2786)
                     /gene="yqjK"
                     /locus_tag="N7A07_001755"
     CDS             complement(2487..2786)
                     /gene="yqjK"
                     /locus_tag="N7A07_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU2476434.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2776..3180)
                     /gene="yqjE"
                     /locus_tag="N7A07_001756"
     CDS             complement(2776..3180)
                     /gene="yqjE"
                     /locus_tag="N7A07_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2476435.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3183..3488)
                     /gene="yqjD"
                     /locus_tag="N7A07_001757"
     CDS             complement(3183..3488)
                     /gene="yqjD"
                     /locus_tag="N7A07_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU2476436.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3526..3894)
                     /gene="yqjC"
                     /locus_tag="N7A07_001758"
     CDS             complement(3526..3894)
                     /gene="yqjC"
                     /locus_tag="N7A07_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU2476437.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(4041..4424)
                     /gene="mzrA"
                     /locus_tag="N7A07_001759"
     CDS             complement(4041..4424)
                     /gene="mzrA"
                     /locus_tag="N7A07_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU2476438.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4428..5090)
                     /gene="yqjA"
                     /locus_tag="N7A07_001760"
     CDS             complement(4428..5090)
                     /gene="yqjA"
                     /locus_tag="N7A07_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU2476439.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5112..5195)
                     /gene="yqiM"
                     /locus_tag="N7A07_001761"
     CDS             complement(5112..5195)
                     /gene="yqiM"
                     /locus_tag="N7A07_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU2476440.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5435..6211)
                     /gene="exuR"
                     /locus_tag="N7A07_001762"
     CDS             complement(5435..6211)
                     /gene="exuR"
                     /locus_tag="N7A07_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU2476441.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6341..7639)
                     /gene="exuT"
                     /locus_tag="N7A07_001763"
     CDS             complement(6341..7639)
                     /gene="exuT"
                     /locus_tag="N7A07_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU2476442.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8122..9534
                     /gene="uxaC"
                     /locus_tag="N7A07_001764"
     CDS             8122..9534
                     /gene="uxaC"
                     /locus_tag="N7A07_001764"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU2476443.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9549..11036
                     /gene="uxaA"
                     /locus_tag="N7A07_001765"
     CDS             9549..11036
                     /gene="uxaA"
                     /locus_tag="N7A07_001765"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU2476444.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11119..11670
                     /gene="ygjV"
                     /locus_tag="N7A07_001766"
     CDS             11119..11670
                     /gene="ygjV"
                     /locus_tag="N7A07_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU2476445.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11675..12919)
                     /gene="sstT"
                     /locus_tag="N7A07_001767"
     CDS             complement(11675..12919)
                     /gene="sstT"
                     /locus_tag="N7A07_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU2476446.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13318..14283)
                     /gene="alx"
                     /locus_tag="N7A07_001768"
     CDS             complement(13318..14283)
                     /gene="alx"
                     /locus_tag="N7A07_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU2476447.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQQVG"
     gene            complement(14567..15553)
                     /gene="ygjR"
                     /locus_tag="N7A07_001769"
     CDS             complement(14567..15553)
                     /gene="ygjR"
                     /locus_tag="N7A07_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2476448.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15632..16324)
                     /gene="ygjQ"
                     /locus_tag="N7A07_001770"
     CDS             complement(15632..16324)
                     /gene="ygjQ"
                     /locus_tag="N7A07_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU2476449.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16401..16904)
                     /gene="ygjP"
                     /locus_tag="N7A07_001771"
     CDS             complement(16401..16904)
                     /gene="ygjP"
                     /locus_tag="N7A07_001771"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU2476450.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16989..18125
                     /gene="rlmG"
                     /locus_tag="N7A07_001772"
     CDS             16989..18125
                     /gene="rlmG"
                     /locus_tag="N7A07_001772"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU2476451.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18409..18723
                     /gene="higB"
                     /locus_tag="N7A07_001773"
     CDS             18409..18723
                     /gene="higB"
                     /locus_tag="N7A07_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGU2476452.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18720..19136
                     /gene="higA"
                     /locus_tag="N7A07_001774"
     CDS             18720..19136
                     /gene="higA"
                     /locus_tag="N7A07_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU2476453.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(19181..21199)
                     /gene="fadH"
                     /locus_tag="N7A07_001775"
     CDS             complement(19181..21199)
                     /gene="fadH"
                     /locus_tag="N7A07_001775"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU2476454.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21725..>24064)
                     /gene="ygjK"
                     /locus_tag="N7A07_001776"
     CDS             complement(21725..>24064)
                     /gene="ygjK"
                     /locus_tag="N7A07_001776"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU2476455.1"
                     /translation="TILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDYDYDD
                     HQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQDGKKV
                     NFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDGELLE
                     KLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEYQVHK
                     SLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAFYLTA
                     SQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVTGRWF
                     SGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDLIAWN
                     LSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWLRNRD
                     HNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDSLEIP
                     AQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLGYSLL
                     QESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQYFYD
                     VRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFNTFVP
                     LGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAKGLTA
                     DGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
BASE COUNT         5910 a         6348 c         6180 g         5626 t
ORIGIN      
        1 ttgtgtaaat gctattcgta tctttctttt tcggatgtta taaaggtttt gatatttaag
       61 ataaatacat ggttattttt ataggtaatt tatataacga cattgaaata ttttaaataa
      121 acattagtaa attattcatt catcaacatg atgataaaag ccggatgagt cagacatccg
      181 gcgagcagat ttgttttaag gttccagttt tggatcaggt ccaaagcggt tttcgcctgg
      241 cgtacccgcc tggcagttaa agacgatgat aataagccag ccgataaacg ggattaagaa
      301 tagcagtgcc caccacgccg agcggtcggt gtcgtgcagg cggcggaact gaactgccca
      361 ccacggtaaa aacaccagaa taccgtagat tgtcgtcagg atgccttcgc caccagcgcg
      421 ttgccagcct aacattttat ccagcagccc cagcacgaac gtaaagatga tgttgaccag
      481 aataaacatc cagtactctt tgcgccgcgc acgaccccgg aaaccgacat aattttttag
      541 tactttcaga taccagtcca tttttgcctc aattttgctc aatcacttgt ttttaaagca
      601 gcaaattaag aatagacgtg aatgaaatgg cagtaaatat tcgttcaagg aattaataaa
      661 gtgctgtgaa ggcgcgctaa gactgatgac aagcgcaaaa ttgccagatg cgctacgctt
      721 atctggcccg cgcggtcatt gcaacatatt gaatataaaa gttttgtagg tttgataagg
      781 cgttcatgcc gcatccgaca tgaacaacat gcacttagct aattatcaga ggcacctcgt
      841 aaggtgcttt ttttaaccta agcgaacatc tcgtccatgc ggttcatcga gatcctgcca
      901 cggaccaatt gaaataatcc gcgtagggtt gatggtctta tgactgcgga agtaatgatt
      961 acggatatga tcgaaattga ctgtttcggc aattcccggc atctggtaga tatcacgcag
     1021 gaagccatac agattaaggt aatcgctgat gcggtgctta tcacacttga agtgggtcac
     1081 atacactgga tcaaaacgga ccagcgtggt ccacaggcga atatcggctt cggttagctg
     1141 gttgccggtc aggtaacgat gctgacctaa aatctgttcc agtcgcgcca gcgattcaaa
     1201 cactttctcc accgcttcgt cgtaggcttg ctggctggtg gcaaaaccag ctttatacac
     1261 gccgttgtta acggtgtcat aaatccagcc gttaagttcg tcaattttcg tttgcagggc
     1321 tggtgggtag taatcacccg ctttcgcgcc cagcgcatca aacgcggtat taaacatgcg
     1381 gatgatttct gctgattcgt tgctgacgat ggtgtggttc tttttgtccc acagcacggg
     1441 aacagtaact cgtccgctgt agtgtggatc ggcgtggaga taaagctgat acaaaaattc
     1501 atgttgatag agcgtgtctc cggttgctcc cggaaaactg tcatcaaagg tccagccgtt
     1561 ttccagcatc agcgggttca ctacggaaac ggaaataaac ggttccagtc ctttgagttt
     1621 gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt gaaacataga gatgataacg
     1681 atctttctct gcgataaaac cgcctgtgcc agtggggcca ggagcgccat cggcagtgag
     1741 ccagttacga aatgcggaag ctgaacgttg aaatttaccg ccggtagatt tggtatcgta
     1801 ccaggtgtca tgccagacgc cgtcaatcag ttgacccatt gttctctcct ccaatagcaa
     1861 aaacgaggag cttcctcctc gttttttatc gttaagtata gcgcgtgctt accacttttt
     1921 attcagcagg cggtcgatgc tatacgcgcc cggaccggta attgccagca gcaggaatcc
     1981 gccagaaatt gtcaggtttt tcatgaacat cagcgagttg acgccttcag caaagttgct
     2041 gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta aacagggctg tggtgcgagt
     2101 caggaaaccg aacaggattg ccagaccacc accaaactca agcagaatca ccagcggcag
     2161 cataaaaccc gggacgccca tcgcttccat atattgttgg gtacccgcgt aaccagtaat
     2221 ttttccccag cctgcggtaa taaacagaat cggcattaaa atgcgcgcta ccagtacacc
     2281 aacatcttct aattttttca tcatactctc cactgcgcca ctcaggctgc tgattgtttt
     2341 tttgtgcaag ggcgcggtat tagcgtcgtt gctgtcgatg gagagaatca taaacgtggt
     2401 gaatgatgat tgttagcaag gaaaactgtc aaaaatcttc aaaaaatttg atgggtaagg
     2461 ccggaatggc tccggccaga gggaagttaa ccgcgaagct gttgctgctt gagggtcgtt
     2521 ttaaccagac gccaggcgct ccatacgcca aaaccgcgtc tggcccagcg gaccagcata
     2581 ttaggatggc gaatcgtcca gatcgccatc acgctactgc caaccagcgc ccaggagcgc
     2641 agacttagca gcatattcca gcgacgatcg taagcgcctg ttgcctccag ccattcacga
     2701 cgactggcag aaagatccag ccgttgttgc tggatctggc taagcagttg cgccttacgt
     2761 cgttcacgtt cgactttact gctcacggga ctcctcctcg agcagctgcc gatcgtttgc
     2821 cagctcatgg cgtgtatggc gcagcaaggt ggatttacgc gatttacgta acgtccagat
     2881 accgccaatc agtgccagta gcagcaacac cacggtggtg gcaatcatcg cattcaggcg
     2941 atattgcgga tcaaccgccc aaataattag caccatcagg ctcataagac caaatgcagc
     3001 gaaaagcatc gtcaggccca gcatcagtaa aagttgaaag agattagctt tttcctcttc
     3061 cagctcaacc accgccagcc gcagacgtgt ttccaccatt tcaaccatga tggaaacaat
     3121 tcgctgcccg atgcccagaa cgcttttacc gggcccttgc gcgtgatgag tgtccgccat
     3181 aattaacgac gcgacagcag aacgccgagc actacaccga ttgcagcgcc aatgcccacg
     3241 cccgtccatg gattttcacg cacatactca tcggcacgcg ccgccgcgac acgggtttgt
     3301 ttggcgatgg catcaccggt ttcacccagg cgataacggc tctgtttcag tgcctgctcc
     3361 gctttgctac gaatcttact caactcttct ttcgacttct cgccagatga gctaagcacc
     3421 tcttccagcg tatcggaaag ggatttcaac tcagcacgca gatgttccgt agtgtgttct
     3481 ttcgacatag ttttctccag gtgagtaagt agtgactatt gtgagttagt agtcgcgcgc
     3541 ttccagcttt ttcagctctt cctgcgcttc tgccagtttc cgttcgcgtt tggcaatctt
     3601 atcggcatcg cctttttgct tcgcctcggc taaatcttgc tggcgttccg ccacctcatc
     3661 tttctgcttt gcgattttct tctgatgatc ggcacgcagc tggctatctg aacagttggc
     3721 ccggacttca ctcagggctt tattcagacc gtcaatacga ttctggtttt ggtgtttttc
     3781 ggcatagctg atctccttaa ggatattttg ctccttttcc tgacacaggg tagtggcata
     3841 actaccggca ctaagagcaa aaagagaaac agctaaagcg atgcggtatt tcattcttga
     3901 accttccatt gtgattcgga cctattacaa aggaacgatt gtccagacaa cgaagcggta
     3961 gtgttaacca ccgcctcatt aatcatagac agttacggtg aaatcaccaa agtgagtgat
     4021 ctgtttcgga caacaggaag ttatccgaag cgatgagagt tatcccgtag ccgggtcagc
     4081 cactgcatag cctgactgtt attgtcctgc tgcgcaatga tgtagccttg gggcaatgtt
     4141 ctgtcgagga ccgcttttgc agcggcgctc tggtcgctgg agtcaaaggt aattaacaga
     4201 gtgtcgtttt tgggggtgat acttttgaaa ggaatgccat gagcgtcaag gtgatgccag
     4261 attgaaaaac cgtctggcat cgttgtgcct tgatgaacgg cacgaatcgc cagcgtagac
     4321 tcttgctggc gaaccgcgga ccaggccagc aacagcgtgc cgaccagaag taaaacagcg
     4381 ccggaccagg ctagctggcg aagcgacatg cgaggtattt gcatccctta cccccgattt
     4441 ccatatttct ttttccataa tacgaccaga gaacctgcca ggccaaacac cagcagcacc
     4501 accgggagca gcatcaggca tgacatcagc tggtcctcgt actttaaaaa taccggcgtt
     4561 ttgccgagca tgtaacccag agttgtcagg atcaataccc acagcagacc gctcatccag
     4621 ttgaaaaact gaaagcgcgc gttattcagc cctgataacc cggcaatcgt cggcagcagt
     4681 gttctgacaa acgcaataaa gcgaccaatt aacagcgccg ataaaccgtg tttatgaaaa
     4741 agatggtgtg cgcgttgatg ataatgcgcg ggtaaatgag atagccagtt ttgtacggtg
     4801 cgggtattgc ccagccatcg cccctgaata tagctgaccc agcagccgag gctggcggca
     4861 acggtcagca gcagaatcgt ttgcggatag cccatcgcgc ctttcgcaat caacacgccg
     4921 accaatacca gtaaactgtc gcccggtaaa aaggccgccg gaagcaagcc gttttcaagg
     4981 aacaaaatta caaacaagac aaaatacaac atgccaatca tcgatggatt ggccagggtt
     5041 tcaaaatcct gcgcccacag ggcttgcagc aattgggtca aaagttccat tcattattcc
     5101 tggtgaatcg gttaacgcca cgctggtttt aaaagatgca ctaccgcaat gttattgctg
     5161 ttttggtatg ttctgcgggt ttgatacatc aacacagcct gaactattcc ctgttaaatc
     5221 gtgagataag aaagcattca aaaacataaa ttcaaattga atctaattgt aacaaaggtc
     5281 aacgttgttc gttatcaaaa ttacatgtcc gtggagtctg attttgctga atgcagggga
     5341 gggtggcgag aggatttaca caggctgaca ctttatactt ttcgcttacc gacgagacaa
     5401 acaggcgcgt cgcagcgcct gtcggacagg tatttcattt actgccactg gtggccgtat
     5461 cgagatgcac taccgggttt tcggcgaaca gatagcggtc ggcattgaac tcgaagtcgt
     5521 cgctggtttc gttgaataac atgatcttgg tgttttccag gtgctgccac attgccagct
     5581 tagcagcatg aggatctttg cgaatcagcg ccttgaggat ttgatcgtgg tcatcacacc
     5641 agttatcgac ggtacgggaa tcaatgtgtt cgtgcagttt tttccagtac gggttatggc
     5701 tacgctgggt ccacattttt tcaacgatag ccgccagggc ggagttctgc gtcgccaggg
     5761 ctacctgaat atggaactgc aaatcccact cggaatcacg gaagcattgt tcgccgcgcg
     5821 cctgctcctg gatggccatc aatttcatga tgtcctgttt cgttacctga gttgctgcga
     5881 actcggcaat attactttcg atgagttggc gagcctggag taactcaaac ggaccgtaat
     5941 tggcgaattc catattattg tccgcagcct gctgatggcg cggctggttg gaaaccacgt
     6001 gaatacctga acctttacgc acttcgacat agccttcaac ttccagcatg atgatggctt
     6061 cacgaactac cgtacggctg acgttctttt catcggcaat aaagcgctca gcaggcagtt
     6121 tatcacccac cagatagacg ccctgttcga tgcgctcttt caggtcagcg gcaagttgtt
     6181 gatacaaacg gcgtggttca gtgatttcca tatgcgctcc aggcatgata cggcagacta
     6241 tatttgttat accacttttg cgagtttagc tctactcgca gtgatgaaaa agccgcctga
     6301 gaaggcggct ttgaagcgaa actaaaactt acacgaagcg ttaatgttgc ggtgcgggat
     6361 cgttatgtgt ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa
     6421 ccagcgcacc cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg
     6481 tgtcagccag tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc
     6541 cggttaaacc gttcgccgtt gccacttcgt tacgaccaaa cacgtcggaa gagagcgtaa
     6601 tcagcgcgcc agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga
     6661 catacgggtt ggtgaacagg ccgatcatac ccgggccaat catcagcact gcacccagcg
     6721 ttacgaccat cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg
     6781 gcaggtaacc accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca
     6841 tcgcgatctc tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt
     6901 taaaagtacc ccaggccggt tctgccagga aacgcggcag cgcgataccc cagaactgac
     6961 ggttacgcag gatctgacca acggacattt tcttcgccgt actcacctgg tgctgggctt
     7021 cctgaccatt aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat
     7081 gtttatagaa aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa
     7141 atgccatctg ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg
     7201 caccaatcga agaacctacg ttaaaatagc ctactgcgat ggaacgctct ttcgccggga
     7261 accattcgga gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg
     7321 caccacgagc aacagccagg ccaccccagc tacctgccag cgcggttgca ccacagaaca
     7381 cggcccacag cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat
     7441 aacctgctac cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat
     7501 actgttgggt ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt
     7561 tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta
     7621 accctttaat tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc
     7681 catcatcccg ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc
     7741 acgccccgaa cgttgccatg aaagtttcgt gctaattcgg cttccgtacc ggttaacccg
     7801 attcatctaa caaatgaatg aatcggcaat ttgcattccg tcgacttatg atttgcgacg
     7861 gcagaaagat aacttgtcat acaactttaa aaggtgagag ccatcacaaa tgtgcgaata
     7921 tttgtaggga cattacctga cgacagcgag gccagtactg gcgcggcctg cagagagatt
     7981 taccactttg agagtaattt ttttaactac gtttattgat ctaactcacg aaaatatctt
     8041 cggactctgg aaattggtgt gataactttg tcagcatcgc accataagca agctagctca
     8101 ctcgttgaga ggaagacgaa aatgactccg tttatgactg aagatttcct gttagatacc
     8161 gaatttgccc gccgtctgta tcacgactac gcaaaagacc agccgatttt cgattaccat
     8221 tgccatttgc cgccgcagca gattgcggaa gactatcgtt ttaaaaacct gtatgacatc
     8281 tggctgaaag gcgatcacta caaatggcgc gctatgcgta ccaacggtgt ggccgagcgt
     8341 ctgtgtaccg gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc gactgttccg
     8401 cacactatcg gcaacccgtt ataccactgg acgcacctcg aactgcgtcg tccgtttggt
     8461 atcactggca aattgctttc tccgtcaact gccgatgaaa tctggaacga atgtaacgaa
     8521 ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg
     8581 gtcggcacca ccgatgaccc gatcgattct ctggagcatc acgcagagat cgccaaagac
     8641 ggctctttca ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt caacatcgaa
     8701 caggcgacct ttaacgacta catggcgaag ctgggcgaag tttccgatac cgacattcgc
     8761 cgctttgctg acctgcaaac tgccttgacc aaacgtctgg atcacttcgc cgctcacggc
     8821 tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa
     8881 ttggacagca ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca cgaagtggca
     8941 cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta
     9001 cagcagtacc acattggcgc gctgcgtaat aacaacctgc gtcagttcaa actgctgggg
     9061 ccggatgtag gctttgactc catcaacgac cgtccgatgg cagaagagtt gtctaagctg
     9121 ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca ttctgtactg cctgaacccg
     9181 cgcgataacg aagtgctggg caccatgagc ggtaacttcc agggcgaagg tatgccgggc
     9241 aaaatgcagt tcggttccgg ctggtggttt aacgatcaga aagacggtat ggaacgtcag
     9301 atgacccaac tggcgcagct cggtttgctg agccgctttg tcggtatgct gactgacagc
     9361 cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc
     9421 ggtcgctggg tggaagcggg cgaagcaccg gcggacatca acctgctggg cgagatggtg
     9481 aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg
     9541 gggttgatat gcaatacatc aagatccatg cgctggataa cgtcgcggtc gctttagcgg
     9601 atttggctga aggcacagaa gtcagtgtcg ataaccaaac tgttaggctg cgccaggatg
     9661 ttgctcgtgg acataaattt gcgttaacga atatcgcaaa aggggccaac gtcatcaaat
     9721 atggcctgcc gattggttat gcattggcgg atattgcggc gggggaacac gttcacgccc
     9781 acaatacgcg cacgaatctg agcgatctgg atcagtatcg ctatcaacct gattttcagg
     9841 atctgcctgc gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg
     9901 tcggggtgcg taacgagctg tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc
     9961 ggcagatcca gaaccgtttt ctgaaagaga ccaacaacgc tgaaggtacc gacggcgtgt
    10021 tcctcttcag ccacacctac ggctgctcac agctgggcga cgatcacatt aatacccgca
    10081 ccatgctgca aaacatggtg cgccacccga acgcaggcgc agtgctggtg attggtctgg
    10141 gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgct gggcgatatc gatcctgaac
    10201 gcgttcattt catgatctgc caacagcagg atgatgagat cgaagccgga atcgagcatt
    10261 tgcatcagct gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac
    10321 tgaagtttgg tctggagtgc ggtggttctg acggtctttc tggtattact gctaacccga
    10381 tgctggggcg tttctctgac tacgtgattg ctaacggcgg tactaccgta ctgaccgaag
    10441 tgccggagat gtttggcgca gagcagttgc tgatggacca ttgccgcgac gaagcaacgt
    10501 ttgaaaaact ggtcaccatg gtcaacgact tcaaacagta ctttattgcc catgaccagc
    10561 cgatctatga aaacccatcg ccggggaaca aagcgggcgg tatcaccacg ctggaagata
    10621 aatcccttgg ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg
    10681 gcgagcgtct gaaaacgccg gggctgaact tgttaagtgc gccgggtaac gatgccgtag
    10741 cgaccagcgc cctggcgggt gcgggctgcc atatggtgct gttcagtact ggtcgtggca
    10801 cgccatacgg cggctttgta ccgacggtga aaatcgctac caacagtgaa ctggcggcga
    10861 agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc
    10921 agttgctgga agaatttatc gataccatcg ttgagtttgc caacggtaaa caaacctgca
    10981 acgagcgtaa cgacttccgc gaactggcga tcttcaaaag cggcgtaacg ctataaagtc
    11041 gaaatgagtt gttaataaaa aacggcgttt cataatgtga agcgccgttt gcttttccat
    11101 acctgataag gatttatcat gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc
    11161 tttctgattg gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt
    11221 tcggtctata gcgccgttat tggcgtacat ttttttcttc tgggcaccta tcccgctggt
    11281 gccagtgcca tccttaatgc cattcgtaca ttgattactt tacgcacgcg cagcttatgg
    11341 gtaatggcga tttttattgt gctgactggc ggaattggcc tcgcgaagtt ccatcatcct
    11401 gtcgaactat tgccggttat cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa
    11461 gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc
    11521 tgggcggggt cgataggcgg cacgatgatt gagggtagtt ttctgcttat gaatggcctg
    11581 aatatcattc gtttctggcg gatgcagaaa aggggaattg atccgtttaa agtagagaaa
    11641 acccctcccg ccatagacga aaggggttaa acaattaatt acgcagggcg ctatttgcca
    11701 gacgatcgtc ttccgcctgg caagctgccg cagtgaacag cacgtcagtt gaagagttca
    11761 gcgcggtttc gcaagagtcc tgcaatacgc cgataataaa gccgacggca accacctgca
    11821 tggcgatatc gttcgaaata ccgaacatat tacaggccag tgggatcagc agcaaagaac
    11881 cccccgccac gccggatgcg ccacaggcac acagagaagc cactacgctc aacagcagcg
    11941 ctgtgggcag atcgaccgga atacccagcg tattaaccgc agccagcgtc aacacggtaa
    12001 tggtgattgc tgcgcccgcc atattgatgg tggctcccag cggaatagaa acggaatagg
    12061 tatcgcgatc cagattcagc ttttcacaca gtgccatatt caccggaatg ttcgccgcag
    12121 agctgcgggt gaagaaggca tacacgccgc tttcgcgcag gcacagcagc accagcggga
    12181 acgggttacg acgaattttc caccacacca gcaatgggtt aaccaccagc gccaccagta
    12241 acatacagcc aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg
    12301 tggttgccag ggtagaagaa accagaccaa aaataccaat cggtgcgaag cgaatgacca
    12361 gtttcaccat aaaggtgacg gcattcgaca tatcgttgac caggtttttg gtggtctcgt
    12421 taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg atgtagttac
    12481 ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat taccagcccg cgcatcactt
    12541 ctacaatgcc tgacggtggc gaaatatcac ccgcgctact ggataagtgc agggtagaag
    12601 ggaaggcgaa gctgaaaacg actgcggcca gagcagcaga gaaggtaccc agcagataga
    12661 ggaacaaaat agggcggata ttggttttct gcccgtgctg gtggttagca atagatgcca
    12721 tcaccagcat caacaccagg atgggggcaa cggctttcag tgcgccgacg aacaaagtac
    12781 ctaacagacc aacagcttcc gccgcgggtt ttgagatcca tgccagaaga atccccagaa
    12841 caaggccgac caggatttgt tttaccaggc tgccatgagc cagacgccgg aatagccccg
    12901 gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg
    12961 ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag
    13021 ttacagcttc gggaagcgaa aaatgctgga ttttaaagag aggtcatatt ttgcaactta
    13081 atggttacat ttatttgaca taaaaataaa gccaacgtgc ggcttccaga ctgaggacta
    13141 cgtcaaaaaa ggactcggtc agtcccctcg ccccgtttgg ggagagggtt agggtgaggg
    13201 gaacaggcca gcatcggtgc gaaaaattac cctcaccccg accctctccc tggaagggag
    13261 agggagaaaa cggtgctcat gttgatgaca gggtacacgg cttaggcaga ttaaaaatca
    13321 tccaacctgc tgcttatcat gccgataatt cacccaggcg ttgataataa acgtcatcac
    13381 caggatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga agtcgacaat
    13441 cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga gcatcgagaa
    13501 acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga tcgcaaacag
    13561 gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg gaatgctatc
    13621 cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca gcggtgtggc
    13681 gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt cggtcatgcg
    13741 caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt catcttcatg
    13801 ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat acaggatcca
    13861 gtcgaactgc gaaatcagcc agctgccagt gaagatcatg atggtacgca gaacaatcgc
    13921 cccgagcacg ccatacacca gcacgcggcg ttgtaatgcc gccggaacgg agaaatagct
    13981 gaacaacatc agccagacaa agacgttatc gaccgccagc gacttctcaa tcagataacc
    14041 cgtgaggaag gccagcgcct gtggatcggc aacggcgcga ccttcggttt gtaccagata
    14101 ccaccagaaa gcagcgttaa acagtaacga cagcgtcacc cagaccagcg accaggccgc
    14161 agcctgtttc atggtcatgg catgtgcccc acgacgtccc tgcaacaaca ggtcgatagc
    14221 cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc cgacagtatt
    14281 catagaagtt ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca gccgctgctt
    14341 tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct
    14401 gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga
    14461 tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc
    14521 agtcaatggt ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc
    14581 tatctgccgg aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttggctgtga
    14641 tgcgactgac cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca
    14701 gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc
    14761 tgccacgcgg cacgaagcac actttctggc attcagacag tttttcaatc accagcgatc
    14821 ccgcttcgcc ctgaatctcg cttgccagga cagaatcgct gacctttgag tgctgcaagg
    14881 tgacgctgaa atcaccgtaa tccatcacca ccacgccgtg ggcatctaca ccacttgcca
    14941 gtaaactggc ggttgcctgc acgcttttcg gctcaccaaa taacgctacc gccgacgcca
    15001 ggcagtaaaa gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg
    15061 gattctcacc gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaaa
    15121 ctttccgcag tttgccgact ttcggcagcg cctggcgtaa cagatgaaag ttaggcaggc
    15181 tggccgtttt aaatgcctca aacagcacca cctgattttc acgcgcgcag gcaatggctg
    15241 catccacttc cgccagattc gacgccagtg gtttctcaca aatcacatga attttgtggc
    15301 tcaggaaaag ttgcgtctgg gaaaaatgca gggcgttcgg gctggcaata tacaccgcgt
    15361 cgatagcgtc actttccgcc attgcttcca gcgaagtaaa cagatgctcg acagaaaact
    15421 cattggcgaa gtgctgggcc tgttcaaggc tgcgggaata aacggcggtt aacttatatt
    15481 taccgctttc atgtgcggcc tccacgaact ggtgagttat ccagttcgta ccaatcacgg
    15541 cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc
    15601 ctttttcatc ttcgacgtgc gcggatacgc attatttctg cgccggacaa ccatgctcac
    15661 taaacggacc aataatgacc gacggcccga gatattttgg ctgacgattg aagattttcg
    15721 cgtcaatcac cgcgctgact ctggcgagtt tctctcgcag acggacaatc tttccgcgtc
    15781 ctttttccag gtctggtgcg ttaaacccga tagcatcgat gccgtattgt tttgccagcc
    15841 agatggcgcg ttggttatga aattcctgcg agataatggt gatatggttc tccccaaaca
    15901 ctttattggc acgcaccact gaatcaagcg ttgagaatcc ggcatagtca cagaagatca
    15961 cttttgccgg tacgcccttt gcgatcaaag cctgctgcat gcctgacgct tcatcgtaat
    16021 ttttacgtcc gttatcgcca ctgaccagca gccatttcac ttttccggcg tggtacaact
    16081 ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctggcc cccagcaata
    16141 agcccacgtt acgcgccgga acgacgttga cgtcgctcca ggtcagctgt ttgctggcgt
    16201 tgaccattac gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg
    16261 caatctttag agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca
    16321 gcatggtcgt tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaagaaaa
    16381 gataacgcct gacatcattt tcagattttg tcctgcccaa gcgacaactg ggttagccac
    16441 agtcgggtgt cgaactcaag ctggtggtac tggggttcca tatgacagca caactggtaa
    16501 aacgctttgt tatgctcttt ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc
    16561 aaaaacggtt ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc
    16621 agcttgccgc cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca
    16681 tggatcttat tgtcatacat cactttattg atcggcgggg cattacgcag aaactgattt
    16741 ttcagatcct gagtatattg ccagagggct ttatcggtgg cgtagtcgtg tgttcccgga
    16801 tagcgttttg ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg cacctgggaa
    16861 agtagctgct cgggataacc ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa
    16921 gagaaaaagg gtatactcac gcaccctttt caggggcaat gtcgaaattt tatcattcag
    16981 gagggccgat gagccactta gacaacggtt tccgttcact gacactacaa cgttttccgg
    17041 cgacggatga cgttaacccg ctacaggcgt gggaagcggc ggatgaatat ttgctgcaac
    17101 agttggacga cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc tttggtgcgt
    17161 taagctgcgc gctggcggaa cataagccgt acagcattgg cgactcatac atcagtgaac
    17221 tggcgacgcg cgagaattta cgcctcaacg ggattgatga gtcgagcgtg aagtttctcg
    17281 acagcaccgc cgactacccg caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac
    17341 tggcattgct ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgcca caaacacgta
    17401 ttattgccgg tgccaaagcc cgtgacatcc acacttccac gctggaacta ttcgaaaaag
    17461 tgctcggccc gaccaccacc acgctggcat ggaagaaagc gcgcctgatt aactgcactt
    17521 tcaatgaacc accgctggcc gatgcgccgc agaccgttag ctggaagctg gaaggtactg
    17581 actggactat ccacaaccat gcgaatgtct tctcccgcac cgggctggat atcggcgcgc
    17641 gcttctttat gcaacatctg ccagagaatc tcgaagggga gattgtcgat ctcggttgcg
    17701 gtaatggcgt tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg
    17761 tcgatgaatc gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc aacatgccag
    17821 aggcgttgga tcgctgcgaa tttatgatca acaacgcgct ctccggcgtg gagcctttcc
    17881 gctttaatgc tgtgctctgc aacccgccgt ttcatcagca acatgcgctg accgataacg
    17941 tcgcctggga gatgttccac cacgcccgcc gctgcctgaa aatcaacggc gagctgtata
    18001 tcgttgccaa ccgtcacctg gattacttcc ataaactgaa gaaaattttc ggcaactgca
    18061 ccaccatcgc cacgaataat aaatttgtgg tgctgaaagc agtgaagctg gggcgtcgtc
    18121 ggtaagattg acagctacct gccagataag gcgtaaacgc cttatctggc atacgtttag
    18181 cacattaccc ccaggtcgga taagatgcgg caagcgtcgc atccggcaat gtctgcccag
    18241 ttctggaatc gcgctccata aaacaaaaaa tccctgtcac catcctctac attctcttgt
    18301 ttagcgttgt ttaacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg
    18361 tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat gcacctgata
    18421 actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac ggagttggtg
    18481 gctctaggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt aaaagcagta
    18541 ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa tgttgggggc
    18601 aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta catacgtgaa
    18661 gttatgacgc ataaagaata cgattttttt accgctgttc atcgtactaa ggggaaaaaa
    18721 tgattgcgat tgccgacatc ttgcatgcag gagaaaagct aactgctgtg gcaccttttc
    18781 tggcgggtat tcagaacgag gaacaataca tccaggcgct ggaactggta gatcatctgc
    18841 tgctcaacga tcctgaaaac cccttactgg atctggtgtg tgccaaaata accgcgtggg
    18901 aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta
    18961 tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat ctgccggaaa
    19021 ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac
    19081 acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacgcg
    19141 acgtgccgga tgcgttgcat ccggcacttc aatgcacgat ttaaatctcc agcgccagcc
    19201 gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg cagccgccaa
    19261 ttaaatgcac cgttttcccg ctatcaatca gcggctgcgc caatgcgcgg tttggctctt
    19321 gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg ttgatcacca
    19381 catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt ttcacacccc
    19441 gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc tgtcccggtt
    19501 tgctggcttt gcgctggagc atcacaatct gccgtgggct acgcgggatc tgcattccct
    19561 gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccattca ttacagaacc
    19621 cggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc gtatcaaaac
    19681 caatcccgcc acaaccgatg atggcaactt tgttgcccac cggcgctttg tcgcgcagta
    19741 catcgagata actcaatacc ttcggatgat cgatcccgtc gatgggcgga gtgcgcggca
    19801 cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc gtcacggtgt
    19861 gattgagttt tagcgtcacg cccgtcactt cgatcatccg gcggtaatag cgcagcgttt
    19921 cgtaaaactc ctctttgcca gggatctgtt tggcgatatt aaactgcccg ccgatctcac
    19981 tgtgcgcgtc aaacaacgtt acctgatgac cacgcgccgc cgcgttaatg gcaaacgcca
    20041 gcccagcagg tcccgcaccg accaccgcca gatttttttt ctgcatggcg ggaaggatcg
    20101 gcattttggt ttcatggcag gcgcgaggat tcaccagaca cgaggtgacc ttgccaacga
    20161 agatctgatc gagacacgcc tggttgcagc caatgcaggt gttgatctca tcggctcgcc
    20221 ctgattgcgc ttttgacagc agttccgcat ccgcaagaaa cggtcgcgcc atcgatacca
    20281 tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta atccggttgg
    20341 tggtcaccag cggcagcgag acgtgacctt tcagtttgcg cgtgacccag ctaaatgcgc
    20401 cgcgtggcac gggcgtggcg atggtcggaa tacgcgcttc atgccagcca atgccggtgt
    20461 tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ctctaccgtt tcggcaaaag
    20521 tcccgccgcc ttcgaccagg tcgagcatcg acagtcggta gataataatg aagtcgttgc
    20581 caacgcgttc gcgtaccgca ctcaccactt caacggcaaa ccgcatccgg ttgtggtaat
    20641 cgccgcccca ctggtcactg cgctgattgg tgcgcagcgt cagaaattcg ttgatcaaat
    20701 acccttcgga acccattacc tctacaccgt cgtatcctgc ctcccgtgcc agttgcgcgc
    20761 agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttca tgaggaacga
    20821 aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg tagctgtagc
    20881 gcccggtatg caaaatttgc agggctattt tgccgccttc ctgatgtacc gcttcggtaa
    20941 tggtgcgatg gtgtgggatc tggctggcgt cattgagcat tgcgccgcct tccatgccaa
    21001 cgcctgttaa atctggtgca atgccgccgc taacaatcag cgccacgccg tgacgggcgc
    21061 gttcggcata aaacgctgcc agccgttcag caccgtccgg gtattcctcc agcccggtgt
    21121 gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc agcggggcga
    21181 acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat gtggtcggat
    21241 gagttctaat ttagccgtcg ccggagaaaa gggaaaaggg gagtgcgggg tttgtgatgg
    21301 gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgg gccctatccg
    21361 acctggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg ccagtggcgc
    21421 ggtgcagacg ccggatgcga cgcttgtcgg gtcttatcca gcctacggga acgaacatgt
    21481 agggcggata aggcgttcac gccgcatccg ccagtggcga ggtgcagatg ccggatgcga
    21541 cgcttgtcgc gtcttatcca gcctacggga acgaacatgt agggcggata aggcgttcac
    21601 gcagcatccg ccggtggcga ggtgcagatg ccggatgcga cgcttgtcgc gtcttatcca
    21661 gcctacggga acgaatatgt agggcggata aggcgttcac gccgcatccg ccagccgttg
    21721 caaattattg cttacggaaa aaatcgttat acaacatata aaaatgtgcg gcactccagg
    21781 agaaatttgg tgcgccttgc tgtgcgcctg tcagcgggtt gtaattttcc tgaattgggc
    21841 catcggcggt taaccctttg gcgtgccgga agaacgtatc cgccagtttc agggcatcat
    21901 cgcgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc acccatacgc
    21961 gcccgcgcca gtagatatca gcgccaaagg ccggattggt taacgccgcc gttcccagcg
    22021 gaacaaaggt attgaactct ttaggatcga gcatcacctt caccaccgcg tcggcattgg
    22081 cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta ccgcgctcaa
    22141 caatcggttt gcccgcgcag ccgttcgcca gcggtttatc ttcaatacgc acgtcataga
    22201 agtactgcgt agccgggtcg aacatacagg tgttgatgta gtccgcgagc tgctgtgcca
    22261 actggcgata gcgcttagcc tcttccggtt taccgagaat cgtcgccatc tccgccagat
    22321 aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat agcgagtacc
    22381 ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt cactgtccag tcgctacgtt
    22441 tgccgccgct tgcgacatat ttatccagct gttctttgtc gataaaccca aagacggcgg
    22501 catcatcacg ccccgattcc cacgatgcag caacctgtgc cggaatttcc agactgtcgt
    22561 actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc tcttctttgt
    22621 tgcctttctt caccgtaaac agcatctcgc cgctctcagt gttgtgggct ttgtcgcggg
    22681 tcgcgccata ttccggcacg ccgttgccgt tgtgatcgcg gttacgtaac caccagtcgt
    22741 gataggccac cagtttcggg tacatctctg ccacccaggt tttatcctgg gtgacgttgt
    22801 acacttccat caccgaccag gcggcaaggc tgggtttggt atttcgttcg ttccagttgc
    22861 caccatcgcc accacgctcg gggctaagat tccacgctat caggtcgggg acaaagccca
    22921 catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc gcgcggatat
    22981 tctctttggc gatgtccgga ttgaaatgcg ccatcgcaaa cgcctgcttc caggtatccc
    23041 acggccaggt ctgattgccg gagaaccagc gcccggtcac cgacggcgtg acggtgttat
    23101 atttcaccgc acccccaggc gagcgccagt taccgttgag cgtttcgatc gctttcaccg
    23161 cgacgcgtgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc agatactctt
    23221 cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata tcgcggatct
    23281 gcatttgctc tttgctaact tcctgagagg tcagcagatg ggaataggtg gtatagagcg
    23341 tcgtcgaacc gttgatatgc gccttactgg taaagcgatt gccgttgatt tcagtctgca
    23401 ccggcaggga tttatgcacc tgatattctg attcaccgga ggtcagcaga tcccaggtgg
    23461 cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt ttgcgctggt
    23521 agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct ttcgcttcca
    23581 gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg atgattttag
    23641 tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc tgcacatctt
    23701 ttgctgtcag tttttgcacc agcgcgccgg gaatattgta tgcctccagc gtgaagttga
    23761 ctttcttgcc gtcctgccag acggtcaggc ggtcgaaatt gctggccata aagttgatgt
    23821 attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg ccgtctggca
    23881 acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg tgatcgtcgt
    23941 aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt ttataattgt
    24001 cggcgttagt ggcatgggcg gaaaaactta tcagcagagc gcaggttact ggcgttaaaa
    24061 tagt
//
LOCUS       DBFIFS010000046        24059 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.046, whole genome shotgun sequence.
ACCESSION   DBFIFS010000046 DBFIFS010000000
VERSION     DBFIFS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.046"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            114..1253
                     /gene="wza"
                     /locus_tag="N7A07_001777"
     CDS             114..1253
                     /gene="wza"
                     /locus_tag="N7A07_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU2476456.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1259..1702
                     /gene="wzb"
                     /locus_tag="N7A07_001778"
     CDS             1259..1702
                     /gene="wzb"
                     /locus_tag="N7A07_001778"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU2476457.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1705..3867
                     /gene="wzc"
                     /locus_tag="N7A07_001779"
     CDS             1705..3867
                     /gene="wzc"
                     /locus_tag="N7A07_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU2476458.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            3960..4799
                     /gene="wcaA"
                     /locus_tag="N7A07_001780"
     CDS             3960..4799
                     /gene="wcaA"
                     /locus_tag="N7A07_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU2476459.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4802..5290
                     /gene="wcaB"
                     /locus_tag="N7A07_001781"
     CDS             4802..5290
                     /gene="wcaB"
                     /locus_tag="N7A07_001781"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU2476460.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5287..6504
                     /gene="wcaC"
                     /locus_tag="N7A07_001782"
     CDS             5287..6504
                     /gene="wcaC"
                     /locus_tag="N7A07_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU2476461.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKGVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6479..7696
                     /gene="wcaD"
                     /locus_tag="N7A07_001783"
     CDS             6479..7696
                     /gene="wcaD"
                     /locus_tag="N7A07_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU2476462.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7707..8453
                     /gene="wcaE"
                     /locus_tag="N7A07_001784"
     CDS             7707..8453
                     /gene="wcaE"
                     /locus_tag="N7A07_001784"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU2476463.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWVELSWHLRQRTTSKTKALYNKS"
     gene            8469..9017
                     /gene="wcaF"
                     /locus_tag="N7A07_001785"
     CDS             8469..9017
                     /gene="wcaF"
                     /locus_tag="N7A07_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU2476464.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9046..10167
                     /gene="gmd"
                     /locus_tag="N7A07_001786"
     CDS             9046..10167
                     /gene="gmd"
                     /locus_tag="N7A07_001786"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU2476465.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10170..11135
                     /gene="fcl"
                     /locus_tag="N7A07_001787"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10170..11135
                     /gene="fcl"
                     /locus_tag="N7A07_001787"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU2476466.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11138..11617
                     /gene="gmm"
                     /locus_tag="N7A07_001788"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11138..11617
                     /gene="gmm"
                     /locus_tag="N7A07_001788"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU2476467.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11614..12837
                     /gene="wcaI"
                     /locus_tag="N7A07_001789"
     CDS             11614..12837
                     /gene="wcaI"
                     /locus_tag="N7A07_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU2476468.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            12840..14276
                     /gene="cpsB"
                     /locus_tag="N7A07_001790"
     CDS             12840..14276
                     /gene="cpsB"
                     /locus_tag="N7A07_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU2476469.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14469..15839
                     /gene="cpsG"
                     /locus_tag="N7A07_001791"
                     /gene_synonym="manB"
     CDS             14469..15839
                     /gene="cpsG"
                     /locus_tag="N7A07_001791"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU2476470.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            15894..17288
                     /gene="wcaJ"
                     /locus_tag="N7A07_001792"
     CDS             15894..17288
                     /gene="wcaJ"
                     /locus_tag="N7A07_001792"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU2476471.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17290..18768
                     /gene="wzxC"
                     /locus_tag="N7A07_001793"
     CDS             17290..18768
                     /gene="wzxC"
                     /locus_tag="N7A07_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU2476472.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            18948..20228
                     /gene="wcaK"
                     /locus_tag="N7A07_001794"
     CDS             18948..20228
                     /gene="wcaK"
                     /locus_tag="N7A07_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU2476473.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20225..21445
                     /gene="wcaL"
                     /locus_tag="N7A07_001795"
     CDS             20225..21445
                     /gene="wcaL"
                     /locus_tag="N7A07_001795"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU2476474.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLSHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21456..22850
                     /gene="wcaM"
                     /locus_tag="N7A07_001796"
     CDS             21456..22850
                     /gene="wcaM"
                     /locus_tag="N7A07_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU2476475.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23008..24003
                     /locus_tag="N7A07_001797"
     CDS             23008..24003
                     /locus_tag="N7A07_001797"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HGU2476476.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
BASE COUNT         5881 a         6021 c         6545 g         5612 t
ORIGIN      
        1 taggactgat gccagttaaa tttttattca attaactgca aggtaaatgt gcatggcgac
       61 attgctatta atagtgcaca ggataattac tctgccaaag tgataaataa acaatgatga
      121 aatccaaaat gaaattgatg ccattattgg tgtcagtaac cttgataagc ggttgcacag
      181 tacttccggg cagcaatatg tcgacgatgg gcaaagacgt catcaaacag caggacgctg
      241 atttcgatct cgacaaaatg gtgaatgttt atccgctgac cccgcgcctg attgaccaat
      301 tacgcccacg cccgaatgta gcgcgcccca atatgacgct ggaaagtgag atcgcgaatt
      361 accagtatcg cgtcgggccg ggggacgttc ttaatgtcac cgtctgggat cacccggaac
      421 tcaccacgcc agccggtcag taccgcagct ccagcgacac cggcaactgg gtacagcctg
      481 acggcactat gttttacccg tatatcggca aggttcacgt agtcgggaaa acgctcgctg
      541 aaatccgcag tgatattacc gggcgcttag cgacgtacat cgctgacccg caggtggacg
      601 ttaatatcgc cgccttccgc tcacaaaagg cctatatctc aggtcaggtg aataaatctg
      661 gtcaacaggc gatcaccaac gtaccactga ccattctcga cgccatcaac gccgcaggtg
      721 gcctgaccga caccgctgac tggcgcaacg tggtgctaac acacaatggt cgtgaagagc
      781 gaatttcttt gcaggcgctg atgcaaaacg gcgacctcaa tcagaaccga ctgctttacc
      841 ccggcgatat tctctacgtg ccgcgtaatg atgatctgaa agtgtttgtg atgggtgaag
      901 tgaagaaaca gagcaccctg aaaatggact ttagcggcat gaccctgacc gaagccctgg
      961 gcaatgctga aggcatcgac atgaccacct ccaacgccag cggcatcttt gtcattcgtc
     1021 cgctgaaagg cgagggcggg cgtaacggca aaattgccaa tatctaccag ctggatatgt
     1081 ccgatgccac gtcgctggtg atggcgacag aattccgcct gcaaccttat gacgtggtgt
     1141 atgtcaccac cgccccggtt tcccgctgga accgtctgat caatcagttg ctgccaacta
     1201 ttagcggtgt ccgttacatg acggatacag ccagcgacat tcataactgg taatcgtcat
     1261 gtttaacaac atcttagttg tctgtgtcgg caatatttgc cgttccccga cggcggaacg
     1321 cttactgcaa cgttatcacc cggagctgaa agtggagtcc gctggactcg gcgcgctggt
     1381 cggtaagggc gctgatccta ccgctatcag cgtcgccgca gaacatcaac tgtctctgga
     1441 aggccactgt gcacgacaaa tcagccgccg cctgtgtcgc aactatgacc tgattttgac
     1501 catggaaaag cgccatatcg aacgcttatg cgagatggca ccggagatgc gcggcaaagt
     1561 gatgctgttt ggtcactggg ataacgaatg tgaaatcccc gatccgtatc gcaaaagccg
     1621 ggaaacgttt gcagcggtgt acacattact tgaacggtct gcccgccagt gggcgcaggc
     1681 attgaacgca gagcaggtat aagaatgaca gaaaaagtaa aacaacatgc cgctccggta
     1741 acgggcagtg atgaaatcga tattggtcgc ctggtcggca ccgtcattga agcgcgctgg
     1801 tgggtgattg gcatcaccgc tgtattcgcc ctttgtgccg tggtttacac cttcttcgcc
     1861 acgccgattt atagtgccga cgcactggta caaatcgagc aaagcagcgg caattcgtta
     1921 gtgcaggata tcggatcggc gttagccaac aaaccgcctg catcggacgc cgagatccag
     1981 ttgattcgtt cgcgcctggt gcttggtaaa acggtggatg atctcgacct cgatattgcg
     2041 gtgagcaaaa acacgttccc gattttcggt gcgggctggg atcgcctgat gggacgccag
     2101 aacgagacgg tgaaagtgac tacctttaac cgtccgaaag agatggagga tcaggtgttt
     2161 acgcttaatg tgctggacaa caaaaactac accctgagca gcgatggcgg ctttagcgcc
     2221 cgtgggcaag cgggccagat actgaaaaaa gaaggcgtca cgctgatggt tgaagccatt
     2281 cacgcccgcc cgggcagtga gtttaccgtc accaaatact ccacgctggg gatgatcaat
     2341 caactgcaaa acagcctgac ggtaacggag aacggcaaag acgcaggcgt actgagcctg
     2401 acttataccg gtgaagatcg cgaacagatc cgcgacattc ttaacagcat cgcccgtaac
     2461 tatcaggaac aaaatattga gcgcaaatcg gcggaagcgt cgaaaagcct cgctttcctc
     2521 gcgcaacagt taccggaagt acgtagccgc cttgatgttg ccgaaaacaa actgaatgcc
     2581 ttccgtcagg ataaagattc tgttgatctg ccgctggaag cgaaagcggt gctcgattcg
     2641 atggtgaaca tcgacgccca gttgaacgaa ctgaccttta aagaggcgga aatctccaag
     2701 ctgtacacca aagttcaccc cgcgtaccgc acgctgctgg agaaacgtca ggcgctggaa
     2761 gacgaaaaag ccaaacttaa tggtcgcgta acggcgatgc cgaaaaccca gcaggaaatt
     2821 gtccgtctga cccgcgatgt cgagtctggt cagcaggtct atatgcaact gctgaataaa
     2881 gagcaggagc tgaaaatcac cgaggccagc accgtcggcg atgtgcgcat tgttgacccg
     2941 gcaatcactc agcctggtgt gctaaaaccg aagaaagggc tgattatcct tggggcgatt
     3001 atccttggcc tgatgctctc tatcgtgggg gtgctgctgc gctcgttgtt taatcgcggc
     3061 atcgaaagcc cgcaggtgct ggaagaacac ggtatcagcg tctatgccag catcccgctg
     3121 tcggagtggc agaaagcgcg cgatagcgtc aaaaccatca aagggattaa acgctataaa
     3181 cagagccagc tactggcggt ggggaatcca accgatctgg cgattgaagc catccgcagc
     3241 cttcgtacca gtttgcactt cgcgatgatg caggcgcaga acaatgtgtt gatgatgacc
     3301 ggggttagcc cgtcaatcgg taaaaccttt gtctgcgcca acctggcggc ggtgatcagc
     3361 cagaccaata aacgcgtgtt gttgatcgac tgcgatatgc gcaaaggcta cacccatgag
     3421 ctgttgggca ctaataacgt taatggcctg tcggaaattc tgattggtca gggcgatatt
     3481 actacagctg ctaaaccgac ctctattgcc aaatttgacc tgatcccgcg cggtcaggta
     3541 ccgccaaatc cttctgaact gttgatgagc gaacgctttg ccgaactggt gaactgggcg
     3601 agtaaaaact acgacctggt gttgattgat acgccgccga ttctggcagt gaccgatgcg
     3661 gcaattgttg gtcgtcatgt cggaaccacg ttaatggtgg cgcgttatgc ggtcaacaca
     3721 ttgaaagaag tggaaaccag tctgagccgc tttgagcaaa acggtattcc ggtgaaaggg
     3781 gtgattctga actccatctt ccgccgcgcc agcgcgtatc aggattatgg ctattacgaa
     3841 tacgaatata agtcggatgc gaaataacga ggcctgcatt cgcaccgccc cgtaggccgg
     3901 ataaggcgct cacgccgcat ccggcaagca aaccagctca taagccggga gtacaaccta
     3961 tgaaagacaa tccgctgatc tcaatctata tgccgacctg gaaccgccaa caactggcga
     4021 ttcgggcgat aaaatcggtc ttgcgccagg actacagcaa ctgggagatg atcatcgtgg
     4081 atgattgctc cacctcctgg gaacaactgc aacagtacgt caccgccctc aacgatccgc
     4141 gtattaccta cattcacaac gacattaaca gcggggcgtg cacggtacgt aaccaggcga
     4201 ttatgctggc gcaaggggaa tacattaccg ggatcgatga cgatgatgaa tggacgccca
     4261 accgcctgag cgtcttcctc gcccataaac agcaactggt cactcacgcc tttttgtacg
     4321 ctaacgatta cgtttgccag ggcgaagttt attcccaacc ggcaagctta ccactgtatc
     4381 ccaaatcgcc gtactcacgc cgcctgttct ataaacgcaa tatcattggc aatcaggtct
     4441 ttacctgggc atggcgtttc aaagagtgtt tgttcgatac cgaactgaaa gcggcacagg
     4501 attacgacat cttcctgcgg atggtggtgg agtatggcga accgtggaaa gtggaagagg
     4561 cgacgcagat cctgcatatc aatcacggtg aaatgcagat cacctcgtca ccgaaaaaat
     4621 tctccggata cttccacttt taccgcaagc acaaagacaa attcgaccgt gccagtaaaa
     4681 aatatcagct gtttaccctc taccagatcc gcaataaacg tatgacctgg cgcactttgc
     4741 tgacgctgct gtcggtgcgt aacggcaaac gtctggctga tggtattcgg gggcgctaaa
     4801 tatgctggaa gatctacgcg ccaacagctg gagtttacgc ccgtgctgca tggttcttgc
     4861 ctatcgcgtc gctcattttt gttcggtgtg gcgcaaaaag aacgttctca acaatctttg
     4921 ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa tgctttttcg gttatgaaat
     4981 ccaggctgcc gcgaccattg gccgccgctt tactatccat cacggttacg ccgtagtgat
     5041 caataaaaac gtggtagcgg gggatgattt caccattcgc cacggcgtca ctatcggcaa
     5101 tcgtggtgcc gataacatgg catgtccaca cattggcaac ggcgtcgaac tcggtgccaa
     5161 cgtcattatt cttggtgata tcacgcttgg taacaacgtc accgtgggcg cgggcagcgt
     5221 ggtgctcgac tctgtcccgg acaacgcgct ggtggtggga gaaaaagcgc gagtgaaggt
     5281 aattaaatga atattctgca atttaatgtg cgactggcgg aaggcggggc ggcaggtgtg
     5341 gcgttagatc tccaccagcg tgcgctgcaa caggggctgg cgtcacattt tgtctacggc
     5401 tacggcaaag gcggcaaaga gagcgtcagc catcagaact atccgcaggt catcaaacat
     5461 acgccgcgga tgaccgcaat ggcgaatatt gccctgtttc gtctgtttaa tcgcgatctg
     5521 tttggcaatt tcaatgagtt atatcgcacc attactcgta caccgggtcc ggtggtcctg
     5581 cattttcatg tgctgcacag ctactggcta aatcttaagg gcgtggtgcg cttttgcgaa
     5641 aaggtgaaaa accacaaacc ggatgtcact ctggtctgga cgctgcacga ccactggagc
     5701 gttaccgggc gctgcgcctt taccgacggt tgtgaaggct ggaaaacggg ctgccagaaa
     5761 tgcccgacct taaataacta tccgccggtg aagattgatc gggcgcacca gctggtggcg
     5821 ggcaaacgcc agttattccg tgagatgctg gcgctgggct gtcagtttat ttcccccagc
     5881 cagcatgtgg ctgacgcttt caatagcctg tacggtccag ggcgttgccg gattatcaat
     5941 aacggtattg atatggcaac cgaggcgatt ctggcggatt tgcctccggt gcgcgaaact
     6001 cagggcaagc cgaaaatcgc ggtggtagcg catgacctgc gttacgacgg caaaactaac
     6061 cagcaactgg tacgcgagat gatggcgctg ggcgacaaaa tcgaactgca tacctttggt
     6121 aagttctcgc cgttcaccgc tggcaacgtg gttaatcacg gctttgaaac tgacaagcgc
     6181 aagttgatga gcgcgctcaa tcagatggat gcgctggtgt tcagttctcg cgtcgataac
     6241 tacccgctga ttttgtgtga ggcgttatca attggcgtgc cggtgattgc cacccatagc
     6301 gacgcggcgc gtgaagtgtt gcaaaaatcc ggcggtaaaa ccgtcagcga agaagaggtg
     6361 ctgcaactgg tccagttaag caaaccggaa atcgcgcagg cgttatttgg taccacgctg
     6421 gctgagttca gccaacgcag ccgcgccgcc tacagtggac aacagatgct ggaggagtat
     6481 gtcaacttct atcagaatct gtagctacct gctgctgccg ctgatctacc tgctggttaa
     6541 cgtcaaaatc acccagcttg gcgaaagttt ccccatcacc atcgtcactt ttttacctgt
     6601 cttgttactg ctgtttttag aacgcatcag cgttaaaaaa ttgatgattg ccttagggat
     6661 ttgcgcggga ctcacggcgt ttaactacct gtttggtcag tcgctggatg ccagtaaata
     6721 cgtcacttca actatgctgt ttgtctatat cgtgatcatt attggtatgg tgtggagtat
     6781 tcgctttaaa acgatttcgc cacacaacca tcgcaagata ttacgtttct tttatctggt
     6841 ggtcgggctg gtggtggcgc tggcggcggt ggagatggcg caaattatcc tcaccggcgg
     6901 cagcagtatt atggagtcga tttcgaaata tctgatttac agcaacagct atgtgctgaa
     6961 tttcatcaaa ttcggcggca agcgcacgac agcactttat ttcgaaccgg catttttcgc
     7021 tctggcatta atctcaattt ggctcagcat caaacagttt ggtatcaaaa cgcctaaaac
     7081 agatgctatg attctcgcag ggataatatt atccggatcg ttttcagggg ttatgacctt
     7141 tatcctgttt tatttgctgg agtgggcatt tcaatatctg aataaagagg cgattaagaa
     7201 aaagttaccg ttagcattga tttctctggc agtattcctg gttggtgtgg taattgcatt
     7261 tccttatatt tccacccgtc tgggcgattt aggtacggaa ggatcgtcat cgtattatcg
     7321 tattgtcggt ccgctggtga tggtcggtta ttctttgacc catattgacg gtgtagtcag
     7381 atttggctca ctttatgaat atgtcgcatc attcggaata tttaacggtg cggatgtcgg
     7441 aaaaaccata gacaatggtt tgtatctgct gattatttat ttttcttggt tcgcggtgtt
     7501 tttatcgctg tggtacatgg ggaaagtgat aaaaatgatg atcaacgctt ttggtgataa
     7561 ccgcaatttt cgcgtgcaat tatatctttt tactccggta tcgctgtttt ttaccggttc
     7621 gatatttagc ccggaatatg catttttaat cgtctgtccg tttattttgc gaaaagcgtt
     7681 aaatattacg aggtaagaat aagaacatgt tgcttagcat aatcactgtc gcgtttcgta
     7741 acctcgaagg gatagtcaaa acacatgcct cgctggcgca tctggcgcag gcggaagata
     7801 tcagcttcga atggattgtt gtcgatggcg gttccaatga cggcactcgt gagtacctgg
     7861 aaaatctcaa tggtatctat aacctacgct ttgtcagcga gccagataac ggtatctacg
     7921 acgccatgaa caagggcatt gcgatggcac aaggcaagtt cgcgttgttt ctcaactcgg
     7981 gcgatatttt tcatcaggat gccgcatatt ttgtccgtaa gttaaaaatg caaaaagata
     8041 acgtgatgat caccggcgat gcgctgctgg attttggcga cgggcataaa attaaacgta
     8101 gcgccaaacc gggctggtat atttatcaca gcctgcccgc cagtcatcag gcgatatttt
     8161 tcccggtatc cggtttgaaa aaatggcgtt atgacctgga atataaagtt tcttctgatt
     8221 acgcgctggc agccaaaatg tataaagcag gttatgcatt taaaaaactc aatggcctgg
     8281 tgtctgaatt ttctatgggt ggggtatcta ccaccaataa tatggaattg tgtgctgacg
     8341 cgaaaaaagt ccaacggcaa atattacatg tgcctggctt ttgggttgaa ttatcctggc
     8401 atttacgcca gcgtactacc tcaaagacga aagccttata taacaaaagc tgaatataag
     8461 ggaaaacgat gcaggattta agcggtttct cggtgccgaa agggttccgg ggcggcaacg
     8521 ctattaaagt gcaattgtgg tgggcagtac aggcaacaat atttgcctgg tcgccacaag
     8581 tattgtatcg ctggcgggca tttttattac gtttattcgg cgcaaaaata ggaaaaaacg
     8641 tagttattcg tccgtcagta aaaattacct atccgtggaa attaacctta ggtgattacg
     8701 tgtgggtcgg cgatgacgtc aatttatata ccctcggtga aataaccatt ggcgcacatt
     8761 cggtgatatc gcaaaaaagt tatttatgca ccgggagcca cgaccatgca agtcaacatt
     8821 tcaccattaa cgccacgcct attgtgattg gcgagaaatg ctggctggca accgatgttt
     8881 ttgttgcccc aggcgtcacg attggcgacg gcaccgtcgt gggtgcgcga agcagtgttt
     8941 ttaaatcgct tccggcaaat gtggtttgcc gggggaatcc cgcagtggtg atacgcaaac
     9001 gcgttgaaac tgaataaatt caaaaaaaat acagaggaat aagacatgtc aaaagtcgct
     9061 ctcatcaccg gtgtaaccgg acaagacggt tcttacctgg cagagtttct gctggaaaaa
     9121 ggttacgagg tgcatggtat taagcgtcgc gcatcgtcat tcaacaccga gcgcgtggat
     9181 cacatttatc aggatccgca cacctgcaac ccgaaattcc atctgcatta tggcgacctg
     9241 agtgatacct ccaacctgac gcgtattttg cgtgaagtgc agccggatga agtgtacaac
     9301 ctgggcgcaa tgagccacgt tgcggtttct tttgagtcac cggaatatac cgctgacgtc
     9361 gacgcgatgg gtacgctgcg cctgctggag gcgatccgct tcctcggtct ggaaaagaaa
     9421 acccgtttct atcaggcttc cacctctgaa ctgtatggtc tggtgcagga aattccgcag
     9481 aaagagacca cgccgttcta tccgcgctct ccgtatgcgg tcgccaaact gtatgcctac
     9541 tggatcaccg tcaactaccg tgaatcctac ggcatgtatg cctgtaacgg tattctcttc
     9601 aaccatgaat ccccgcgccg cggcgaaacc ttcgttaccc gcaaaatcac ccgcgcaatc
     9661 gccaacatcg cccaggggct ggagtcgtgc ctgtacctcg gcaatatgga ttccctgcgt
     9721 gactggggcc atgccaaaga ctacgtaaaa atgcagtgga tgatgctgca acaggaacag
     9781 ccagaagatt tcgttatcgc gaccggcgtt cagtactccg tacgtcagtt cgtagaaatg
     9841 gcggcagcac agctgggcat caaactgcgc tttgaaggca cgggcgttga agagaagggc
     9901 attgtggttt ccgtcaccgg gcatgacgcg ccgggtgtta aaccgggtga tgtgattatc
     9961 gccgttgatc cgcgttactt ccgtccggct gaagttgaaa cgctgctcgg cgacccgacc
    10021 aaagcgcacg aaaaactggg ctggaaaccg gaaatcaccc tcagagagat ggtgtctgaa
    10081 atggtggcta atgacctcga agcggcgaaa aaacactctc tgctgaaatc tcacggctac
    10141 gacgtggcga tcgcgctgga gtcataagta tgagtagaca acgcattttt attgctggcc
    10201 atcgcgggat ggtcggttcc gccatcaggc ggcagctcga acagcgcggt gatgtggaac
    10261 tggtattacg cacccgcgac gagctgaacc tgctggacag ccgcgccgtg catgatttct
    10321 ttgccagcga acgtattgac caggtctatc tggcggccgc gaaagtgggc ggcattgttg
    10381 ccaacaacac gtatccggcg gatttcatct atcagaacat gatgattgag agcaacatca
    10441 ttcacgccgc gcatcagaac gacgtgaaca aactgctgtt tctcggatcg tcctgcatct
    10501 acccgaaact ggcaaaacag ccgatggcag aaagcgagtt attgcagggc acgctggagc
    10561 cgaccaacga gccttacgcc attgccaaaa tcgccgggat caaactgtgc gaatcataca
    10621 accgccagta cggacgcgat taccgctcag tcatgccgac caacctgtat ggcccgcatg
    10681 acaacttcca cccgagtaat tcgcatgtga tccctgcatt gctgcgtcgc ttccacgagg
    10741 cgacggcaca gaatgcaccg gacgtggtgg tgtggggcag cggtacgccg atgcgagaat
    10801 ttctgcacgt cgatgatatg gcggcggcga gcattcatgt catggagctg gcgcatgaag
    10861 tctggctgga gaacacccag ccgatgctgt cgcacattaa cgtcggcacg ggcgttgact
    10921 gcactatccg cgagctggcg caaaccatcg ccaaagtggt gggttacaaa ggtcgggtgg
    10981 tttttgatgc cagcaaaccg gatggtacgc cgcgcaaact gctggatgtg acgcgcctgc
    11041 atcagcttgg ctggtatcac gaaatctcac tggaagcggg gcttgccagc acttaccagt
    11101 ggttccttga gaatcaagac cgctttcggg ggtaatgatg tttttacgtc aggaagactt
    11161 tgccacggta gtgcgctcca caccgcttgt ctctctcgac tttattgtcg agaacagtcg
    11221 cggcgagttt ctgcttggca aaagaaccaa ccgcccggcg cagggttact ggtttgtgcc
    11281 gggagggcgc gtgcagaaag acgaaacgct cgaagccgca tttgagcggc tgacgatggc
    11341 ggaactgggg ctgcgtctgc cgataacagc aggccagttt tacggtgtct ggcagcactt
    11401 ttatgacgat aacttctccg gcacggattt caccactcac tatgtggtgc tcggttttcg
    11461 cttcagagta gcggaagaag aactgttact gccggatgag cagcatgacg attaccgctg
    11521 gctgacgccg gacgcgctgc tcgccagtaa cgatgttcac gctaacagcc gcgcctattt
    11581 tctcgctgag aagcgtgccg gagtacccgg attatgaaaa tactggtcta cggcattaac
    11641 tactcgccgg agttaaccgg catcggcaaa tacaccggcg agatggtgga atggctggcg
    11701 gcgcaaggtc atgaggtgcg ggttattacc gcaccgcctt actacccgca gtggcaagtg
    11761 ggcgagaact attccgcctg gcggtacaaa cgagaagagg gggccgctac ggtgtggcgc
    11821 tgcccgctgt acgtgccaaa acagccgagc accctgaaac gcctgttgca tctcggcagt
    11881 tttgccgtca gcagtttttt cccgttgatg gcgcaacgtc gctggaagcc ggatcgcatt
    11941 atcggcgtag tgccaacgct gttttgcacg ccgggaatgc gcctgctggc gaaactctct
    12001 ggcgcgcgta ccgtgctgca tattcaggat tacgaagtag atgccatgct ggggctgggc
    12061 cttgccggaa aaggcaaagg cggcaaagtg gcacagctgg caacggcgtt cgaacgtagc
    12121 ggactgcata acgtcgataa cgtttccacg atttcgcgtt cgatgatgaa taaagccatc
    12181 gaaaaaggcg tggcggcgga aaacgtcatc ttcttcccca actggtcgga aatcgcccgt
    12241 tttcagcatg ttgcagacgc cgatgttgat gcccttcgta accagcttgg cctgccggat
    12301 aacaaaaaaa tcattcttta ctccggcaat attggtgaaa agcaggggct ggaaaacgtt
    12361 attgaagcag ccgatcgcct gcgcgatgaa ccgctgattt ttgccattgt cgggcagggc
    12421 ggcggcaaag cgcggctgga aaaaatggcg cagcagcgtg gactgcgcaa catgcaattt
    12481 ttcccgctgc aatcgtatga cgctttaccc gcactgctga agatgggcga ttgccatctg
    12541 gtggtgcaaa aacgcggcgc ggcagatgcc gtattgccgt cgaaactgac caatattctg
    12601 gcagtaggcg gtaacgcggt gattactgct gaagcccaca cagaactggg acagctttgc
    12661 gaaacctttc cgggcattgc ggtttgcgta gaaccggaat cggttgaggc gctggtggcg
    12721 gggattcgtc aggcgctcct gctgcccaaa cacaacacgg tggcacgtga atatgccgaa
    12781 cgcacgcttg ataaagagaa cgtgttacgt caatttataa atgatattcg gggataatta
    12841 tggcgcagtc gaaactctat ccagttgtga tggcaggtgg ctccggtagc cgcttatggc
    12901 cgctttcccg cgtactttat cccaagcagt ttttatgcct gaaaggcgat ctcaccatgc
    12961 tgcaaaccac catctgccgc ctgaacggcg tggagtgcga aagccctgtg gtgatttgca
    13021 atgagcagca ccgctttatt gttgcggaac aactgcgtca actgaacaaa ctcactgaga
    13081 acattattct cgaaccggca gggcgtaaca cagcaccggc cattgcgctg gcggcgctgg
    13141 cggcaaaacg tcatagcccg gagaacgacc cattaatgct ggtgctggcg gcggatcatg
    13201 tgattgccga tgaagacgca ttccgcgccg ccgtgcgtaa cgctatgccg tatgccgaag
    13261 cgggcaagct ggtgaccttc ggcattgtgc cggatctacc tgaaaccgga tatggctata
    13321 ttcgtcgcgg tgaagtgtcg gcgggtgagc aggatacggt ggcctttgaa gtggcgcagt
    13381 ttgtcgaaaa accaaatctg gaaaccgcgc aggcctatgt ggcaagcggc gaatattact
    13441 ggaacagcgg tatgttcctg ttccgtgccg gacgctatct cgaagaactg aaaaaatatc
    13501 gcccggatat tctcgacgcc tgtgaaaaag cgatgagcgc cgtcgatccg gatctcgatt
    13561 ttattcgcgt ggatgaagac gcgtttctgg cctgcccgga agagtcggtg gattacgcgg
    13621 tcatggaacg tacggcagat gccgttgtgg tgccgatgga tgcgggctgg agcgatgtcg
    13681 gctcctggtc ctcattatgg gagatcagcg cccacaccgc cgagggcaac gtttgccacg
    13741 gcgatgtgat taatcacaaa actgaaaaca gctatgtgta tgccgaatct ggcctggtca
    13801 ccaccgtcgg ggtgaaagat ttggtggtag tgcagaccaa agatgcggtg ctgattgccg
    13861 accgtaacgc ggtgcaggat gtgaaaaaag tggtcgagca gatcaaagcc gatggtcgcc
    13921 atgagcatcg ggtgcatcgc gaagtctatc gcccgtgggg caaatatgac tctatcgacg
    13981 cgggcgaccg ctaccaggtg aaacgcatca ccgtgaaacc gggcgaaggc ttgtcggtac
    14041 agatgcacca tcaccgcgcg gaacactggg tggttgtcgc gggaacggca aaagtcacca
    14101 ttgacggtga tatcaaactg cttggtgaaa acgagtcgat ttatattccg ctgggggcga
    14161 cgcactgcct ggaaaacccg gggaaaattc cgctcgattt aattgaagtg cgctccggct
    14221 cttatctcga agaggatgat gtggtgcgct tcgcggatcg ctacggacgg gtgtaaacgt
    14281 cgcatcaggc gctgattgtc ggatgcggcg tgaacgcctt atccgaccta cggttcggtt
    14341 ttgtaggcct gataagacgc ggcagcgtcg catcaggcaa tgaatgcgaa accgcggtgt
    14401 aaataacgac aaaaacaaaa ttggccgttt cggtcagggc caactattgc ctgaaaaagg
    14461 gtaacgatat gaaaaaatta acctgcttta aagcctatga tattcgcggg aaattaggcg
    14521 aagaactgaa tgaagatatt gcctggcgca ttggtcgtgc ctatggcgaa tttctcaaac
    14581 cgaaaaccat tgtgttaggc ggtgatgccc gtctcaccag cgaaacctta aaactggcgc
    14641 tggcaaaagg tttacaggat gcgggcgttg acgtgctgga tattggtatg tccggcaccg
    14701 aagagatcta tttcgccacg ttccatctcg gcgtggatgg cggcattgaa gttactgcca
    14761 gccataaccc gatggattac aacggcatga aactggtgcg cgaaggggct cgcccgatca
    14821 gcggcgatac cggactgcgc gatgtccagc gtctggcaga agccaacgac tttcctcccg
    14881 ttgatgaaac caaacgcggt cgctatcagc aaatcaatct gcgtgacgct tacgttgatc
    14941 acctgttcgg ttatatcaac gtcaaaaacc ttacgccgct caagctggtg atcaactccg
    15001 ggaacggcgc ggcgggtccg gtggtggacg ccatcgaagc ccgctttaaa gccctcggcg
    15061 caccggtgga attgatcaaa gtgcacaaca cgcccgacgg caatttcccc aacggtattc
    15121 ctaacccgct gctgccggaa tgtcgcgacg acacccgcaa tgcggtcatt aaacacggcg
    15181 cggatatggg cattgctttt gatggcgatt ttgaccgctg tttcctgttt gacgaaaaag
    15241 ggcagtttat cgagggctac tacattgtcg gtctgctggc agaagcgttc ctcgaaaaaa
    15301 atcccggcgc gaagatcatc cacgatccac gtctctcctg gaataccgtt gatgtggtga
    15361 ctgccgcagg cggcaccccg gtaatgtcga aaaccggaca cgcctttatt aaagaacgga
    15421 tgcgcaagga agacgccatc tacggtggcg aaatgagcgc ccaccattac ttccgtgatt
    15481 ttgcttactg cgacagcggc atgatcccgt ggctgctggt cgccgaactg gtgtgcctga
    15541 aaggaaagac gctgggcgaa ctggtgcgcg accggatggc agcgtttccg tcaagcggtg
    15601 agatcaacag caaactggcg caacccgttg aggcgattaa ccgcgtggaa cagcatttta
    15661 gccgtgaggc gctggcggtg gatcgcaccg atggcatcag catgaccttt gccgactggc
    15721 gctttaatct gcgctcctcc aacaccgaac cggtggtgcg gttgaatgtg gaatcacgcg
    15781 gtgatgtgcc gctgatggaa gcgcgaacgc gaactctgct gacgttgctg aacgagtaat
    15841 gtcggatctt cccttacccc attgcgggta aggggctaat aacaggaaca acgatgacaa
    15901 atctaaaaaa gcgcgagcga gcgaaaacca atgcatcgtt aatctctatg gtgcaacgct
    15961 tttcagatat caccatcatg tttgccggac tatggctggt ttgcgaagtg agcggactgt
    16021 cattcctcta catgcacctg ttggtggcgc tgattacgct ggtggtgttc cagatgctgg
    16081 gcggcatcac cgatttttat cgctcatggc gcggtgttcg ggcagcgaca gaatttgccc
    16141 tgttgctaca aaactggacc ttaagcgtga ttttcagcgc cggactggtg gcgttcaaca
    16201 atgatttcga cacgcaactg aaaatctggc tggcgtggta tgggctgacc agtatcggac
    16261 tggtggtttg ccgttcatgt attcgcattg gggcgggctg gctgcgtaat catggctata
    16321 acaagcgcat ggtcgcggtg gcgggggatt tagccgccgg gcaaatgctg atggagagct
    16381 tccgtaacca gccgtggtta gggtttgaag tggtgggcgt ttaccacgac ccgaaactgg
    16441 gcggcgtttc taacgactgg gcgggtaacc tgcaacagct ggtcgaggat gctaaagcag
    16501 gcaagattca taacgtctat atcgcgatgc aaatgtgcga cggcgcgcga gtgaaaaaac
    16561 tggtccatca actggcggac accacctgtt cggtgctgct gatccccgat gtctttacct
    16621 tcaacattct ccattcacgt cttgaagaga tgaacggcgt tccggtggtg ccgctttacg
    16681 acacgccgct ttccggggtt aaccgcctgc tcaaacgtgc ggaagacatt gtgctggcga
    16741 cgcttatcct gctgctgatc tccccggtgc tgtgctgcat tgcgctggcg gtgaaactca
    16801 gttcacctgg gccggttatt ttccgccaga ctcgctacgg catggatggc aagccgatca
    16861 aagtgtggaa gttccgttcc atgaaagtga tggagaacga caaagtggtg acccaggcga
    16921 cgcagaacga tccgcgcgtc accaaagtgg ggaactttct gcgccgcacc tcgctggatg
    16981 aattgccgca gtttatcaat gtgctgaccg gggggatgtc gattgtcggt ccacgtccgc
    17041 acgcggtggc gcataacgaa cagtatcgac agctcattga aggctacatg ctgcgtcata
    17101 aggtgaaacc gggcattacc ggctgggcgc agattaacgg ctggcgcggc gaaaccgaca
    17161 cgctggagaa aatggaaaaa cgcgtcgagt tcgaccttga gtacatccgc gaatggagcg
    17221 tctggttcga tatcaaaatc gttttcctga cggtattcaa aggattcgtt aacaaagcgg
    17281 catattgata tgagcttacg tgaaaaaacc atcagcggcg cgaagtggtc ggcgattgcc
    17341 acggtgatca tcatcggcct cgggctggtg cagatgaccg tgctggcgcg gattatcgac
    17401 aaccaccagt tcggtttgct taccgtgtcg ctggtgatca tcgcgctggc agatacgctt
    17461 tctgacttcg gtattgctaa ctcgattatt cagcgaaaag aaatcagcca ccttgaactc
    17521 accacgctgt actggctgaa cgttgggctg gggcttgtgg tgtgtgtggc ggtgtttttg
    17581 ttgagtgatc tcattggtga cgtgctgaac aacccggacc tggcaccgtt gattaaaaca
    17641 ttgtcgctgg cgtttgtggt tatcccccac gggcaacagt tccgcgcgtt gatgcaaaag
    17701 gagctggagt tcaacaaaat cggcatgatc gaaaccagcg cggtactggc gggcttcact
    17761 tttacggtgg ttagcgccca tttctggccg ctggcgatga ccgcgatcct cggttatctg
    17821 gtcaatagtg cggtgagaac gctgctgttt ggctactttg gccgcaaaat ttatcgcccc
    17881 ggtctgcatt tctcgctggc gtctgttgcg ccgaacttac gctttggtgc ctggctgacg
    17941 gcggacagca tcatcaacta tctcaatacc aacctttcaa cgctggtgct ggcgcgtatt
    18001 ctcggcgcgg gcgtggcagg gggatacaac ctggcgtaca acgtggccgt tgtgccgccg
    18061 atgaagctga acccgatcat cacccgtgtg ttgtttccgg cattcgccaa aattcaggac
    18121 gataccgaaa agctgcgcgt caacttctac aagctgctgt cggtggtggg gattatcaac
    18181 tttccggcgc tgctcgggct gatggtggtg tcaaataact ttgtaccgct ggtatttggc
    18241 gaaaagtgga acagcattat tccggtgctg caattgctgt gtgtggtggg tctgctgcgc
    18301 tccgtaggta acccgattgg ttcgctgctg atggcgaaag cgcgggtcga tatcagcttt
    18361 aaattcaacg tattcaaaac atttctgttt attccggcga ttgttattgg tgggcagatg
    18421 gcgggcgcaa tcggcgtcac gctcggtttc ctgctggtgc agattatcaa caccattctg
    18481 agttacttcg tgatgattaa accggttctt ggttccagtt atcgccagta catcctgagt
    18541 ttgtggctgc cgttttatct ctcgctgccg acgctggtgg tcagttatgt gctgggcatt
    18601 gtgctgaaag ggcaactggc gctggggatg ctgctggcgg tgcaaatagc cgcgggcgtg
    18661 ctggcgtttg tggtgatgat tgtgctgtcg cgccatccgc tggtggtgga agtgaagcgc
    18721 cagttttgtc gcagcgaaaa aatgaaaatg cttttacggg cggggtgagt tgttgttttc
    18781 ggttccgaca acggttgccg ggtgcagcgt aaaacacctt atccatccta cggttctgtg
    18841 ctcgggtctt gtaggcctga taagacgcgc cagcgtcgca tcaggcaaaa aacggcaacg
    18901 aacacaacaa acacagcctc acctggcatc aaatttatga ggaaaaaatg aaattactta
    18961 ttctgggcaa ccacacttgc ggcaatcgtg gcgacagcgc catcttgcgc ggcttacttg
    19021 atgccatcaa catcctcaat ccacataccg aagtggacgt gatgagccgc tatccggtca
    19081 gttcttcctg gctgctcaac cgcccggtaa tgggtgatcc gctgttcctt caaatgaaac
    19141 aacacaacag cgcggcgggc gttgtcgggc gcgttaaaaa agtcctccgt cgccgctatc
    19201 agcaccaggt attgctctca cgcgtcaccg acactggcaa gctgcgcaat atcgccatcg
    19261 cccagggatt caccgacttc gtgcgcctac tgtcaggtta cgacgccatt attcaggtcg
    19321 gcggatcgtt ttttgtcgat ctctacggcg tgccgcagtt tgaacatgca ctttgcacat
    19381 ttatggcgaa aaagccgctg tttatgattg gtcacagcgt cggtcccttc caggatgagc
    19441 aatttaacca actggcgaac tacgtttttg gtcactgcga cgcgctgatc ttgcgcgaat
    19501 cggtcagcct cgatctaatg aaacgcagca atatcaccac tgcaaaagtt gaacatggcg
    19561 tcgataccgc gtggctggtc gatcaccaca cagaagactt caccgccagc tatgccgtcc
    19621 aacactggct ggacgttgcc gcacaacaga aaacggtggc aattaccctg cgcgaactgg
    19681 caccgttcga caaacgcctc ggcaccactc agcaagtgta tgaaaaagcc tttgccgggg
    19741 tggtcaatcg cattctcgac gaaggctatc aggtcattgc gctctccacc tgtacgggta
    19801 tcgacagcta taacaaagac gaccgcatgg tggcgctcaa cctgcgccag cacatcagcg
    19861 atcctgcccg ttatcacgta gtgatggatg aacttaacga tctggaaatg ggcaaaattc
    19921 tcggtgcctg tgaactcacc gtcggtacgc gcctgcactc cgccattatc tcaatgaact
    19981 ttgctactcc ggcgattgcc atcaactatg aacataaatc cgccggggtt atgcagcagc
    20041 tgggactacc ggagatggca attgatatcc gtcatttatt agacggcagc ctgcaagcga
    20101 tggttgcgga taccttaggc cagcttccgg cgctgaacgc acgacttagc gaagccgtca
    20161 gtcgtgagcg tcagacggga atgcagatgg tgcagtccgt actggaacgc atcggggagg
    20221 tgaaatgaag gtcggcttct tcttattgaa atttccgctg tcgtcagaaa ccttcgttct
    20281 taaccaaatc actgcgttta ttgatatggg ctttgaagtg gagattgtcg cgctgcaaaa
    20341 aggcgacaca caaaacaccc acgtggcatg gactaaatac aaccttgccg ccagaacccg
    20401 ctggttacag gacgaaccta cgggcaaagt ggcgaaactg agccaccgag ccagtcagac
    20461 cttgcgcggc attcatcgta aaaatacctg gcaggcgctc aacctcaaac gctatggtgc
    20521 cgagtcgtgg aacctgattt tgtctgccat ttgcggccag gtcgcaacac cgtttcgcgc
    20581 cgatgtgttc atcgctcatt ttggccctgc gggggtaacc gcagcaaaac tacgcgaact
    20641 gggtgtcatt cacggcaaaa ttgccactat tttccacggc attgatattt ccagtcggga
    20701 agtgctcaac cattacactc ccgaatatca acaactgttt cgacgtggcg acctgatgct
    20761 accgataagc gatctgtggg ccggaaggct gcaaaaaatg ggctgcccga gggaaaaaat
    20821 cgccgtatcg cgcatgggcg tagatatgac gcgctttagc ccgcgtcccg tgaaagcgcc
    20881 cgcaacaccg ctggagatta tttccgtcgc acgcttaacc gagaaaaaag gtctgcatgt
    20941 ggcgattgaa gcctgccggc agttgaaaga gcagggcgtg gcatttcgct atcgcatcct
    21001 cggcattggc ccgtgggaac gacgcctgcg cacgctcatc gaacaatatc aactggaaga
    21061 tgtggtggag atgccgggct ttaaaccgag ccatgaagtg aaagcgatgc tcgacgacgc
    21121 ggatgtcttc ctgttgccat cggttacagg tgcggatggc gatatggaag gcattccggt
    21181 ggcgctgatg gaggcgatgg cggtcggcat tccggtggtt tctactctgc atagcggaat
    21241 accggaactg gtggaggctg acaaatccgg ctggctggtg cctgagaacg atgctcgcgc
    21301 actggcgcaa cgactggcgg cgtttagcca actggacacc gacgaattgg ctccggtcgt
    21361 caaacgcgcg cgcgaaaaag ttgaacacga ttttaaccag caggtgatca atcgagaact
    21421 cgccagcttg ctgcaggctt tatagaggag aacgtatgcc atttaaaaaa ctctcccgac
    21481 gcaccttcct gacggcaagc tcggcgcttg ccttccttca tacccctttc gctcgcgcat
    21541 ttcccgcccg acaaagcgtt aatatcaacg actacaaccc tcacgactgg atcgcctcat
    21601 ttaaacaagc cttcagcgaa gggcaaacgg ttgtcgtgcc tgctggattc gtttgtgaca
    21661 atatcaacac cggcatcttc attcctcctg gcaaaacgtt acacatcctt ggaagcctgc
    21721 gcggcaacgg cagagggcga tttgtcttac aggacggcag ccaggtgaca ggggaggagg
    21781 gcggcagtat gcataacatc accctggatg tgcgtggttc tgactgcacc atcaaagggc
    21841 tggcgatgag cggctttggt ccggtaacgc agatttatat cggcggcaaa aacaaacggg
    21901 tcatgcgcaa cctgaccatc gataacctga ccattagcca cgctaattac gccatcttac
    21961 gccagggatt tcataaccag attatcggtg ccaacatcac caattgtaag ttcagcgact
    22021 tacagggcga tgccattgaa tggaacgtgg cgattaacga ccgcgatatt ttgatctccg
    22081 accatgtcat cgagcgcatc aactgtacta acggcaaaat caactggggc attggcatag
    22141 gccttgcggg aagcacttac gataataatt acccggaaga tcaggcagtg aaaaactttg
    22201 tcgtggcgaa tatcacggga tcggattgtc ggcagttgat acatgttgaa aatggtaaac
    22261 attttgttat tcgtaatatc aaagcccgca atatcacgca ggatttcagt aagaaagcag
    22321 gtattgataa cgccacagtc gctatttacg gttgtgacaa tttcgtgatt gataatattg
    22381 aaatgattaa tagcgccggg atgttaatcg gctatggggt aattaaaggc aattatctct
    22441 cgattccgca aaatttccga gtgaataata ttcaactgga taacacccat cttgcttata
    22501 aattgcgcgg cctccaaatc tctgccggga atgccgtctc ctttgtggca ctaactaaca
    22561 ttgagatgaa gcgtgcgtcg ctggagttac acaacaaacc gcaacatctt tttctgcgta
    22621 atatcaaagt gatgcaggaa tcctctgttg gacccgcatt gattatgaac ttcgacatgc
    22681 gcaaagacgt tcgaggcgtc tttatggcga aagaagaaac actgctgtct cttgcaaatg
    22741 ttcatgcggt gaatgagaaa ggacaaagct ccgtcgatat cgacaggatt aatcaccata
    22801 ttgttaatgt ggaaaagatt aactttagat tgccggaacg gagagagtag atttgcgacc
    22861 attcataaac ttactggata taatgttcta aaagattata atcctcacac taactgttaa
    22921 agctggggct gggcagcatt gactactcag ggaaggtact catctttatc ttgtgtttct
    22981 tacgtaacaa ccagactaaa gtgatttatg aacgataacg ttttgctcat aggagcttcc
    23041 ggattcgtag gaacccgact acttgaaacg gcaattgctg actttaatat caagaacctg
    23101 gacaaacagc agagccactt ttatccagaa atcacacaga ttggtgatgt tcgtgatcaa
    23161 caggcactcg accaggcgtt agccggtttt gacactgttg tactactggc agcggaacac
    23221 cgcgatgacg tcagccctac ttctctctat tatgatgtca acgttcaggg tacccgcaat
    23281 gtgctggcgg ccatggaaaa aaatggcgtt aaaaatatca tctttaccag ttccgttgct
    23341 gtttatggtt tgaacaaaca caaccctgac gaaaaccatc cacacgaccc tttcaaccac
    23401 tacggcaaaa gcaagtggca ggcggaggaa gtgctgcgtg aatggtataa caaagcacca
    23461 acagaacgtt cattaactat catccgtcct accgttatct tcggtgaacg caaccgcggt
    23521 aacgtctata acttgctgaa acagatcgct ggcggcaagt ttatgatggt gggcgcaggg
    23581 actaactata agtccatggc ttatgttgga aacattgttg agtttatcaa gtacaaactg
    23641 aagaatgttg ccgcaggtta cgaggtttat aactacgttg ataagccaga cctgaacatg
    23701 aaccagttgg ttgctgaagt tgaacaaagc ctgaacaaaa agatcccttc tatgcacttg
    23761 ccttacccac taggaatgct gggtggatat tgctttgata tcctgagcaa aattacgggc
    23821 aaaaaatacg ctgtcagctc tgtgcgcgtg aaaaaattct gcgcaacaac acagtttgac
    23881 gcaacgaaag tgcattcttc aggttttgtg gcaccgtata cgctgtcgca aggtctggat
    23941 cgaactctgc agtatgaatt cgtccatgcc aaaaaagacg acataacgtt tgtttctgag
    24001 taatgcagac gagtcagaga aaaaaggacc aggttggact acgattattc ggaaagagg
//
LOCUS       DBFIFS010000047        24040 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.047, whole genome shotgun sequence.
ACCESSION   DBFIFS010000047 DBFIFS010000000
VERSION     DBFIFS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.047"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            149..484
                     /locus_tag="N7A07_001798"
     CDS             149..484
                     /locus_tag="N7A07_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU2476477.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            743..1186
                     /gene="yjdN"
                     /locus_tag="N7A07_001799"
     CDS             743..1186
                     /gene="yjdN"
                     /locus_tag="N7A07_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU2476478.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1319..2107
                     /gene="phnC"
                     /locus_tag="N7A07_001800"
     CDS             1319..2107
                     /gene="phnC"
                     /locus_tag="N7A07_001800"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU2476479.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2132..3148
                     /gene="phnD"
                     /locus_tag="N7A07_001801"
     CDS             2132..3148
                     /gene="phnD"
                     /locus_tag="N7A07_001801"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2476480.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3254..4033
                     /gene="phnE"
                     /locus_tag="N7A07_001802"
     CDS             3254..4033
                     /gene="phnE"
                     /locus_tag="N7A07_001802"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU2476481.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4054..4779
                     /gene="phnF"
                     /locus_tag="N7A07_001803"
     CDS             4054..4779
                     /gene="phnF"
                     /locus_tag="N7A07_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU2476482.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4780..5232
                     /gene="phnG"
                     /locus_tag="N7A07_001804"
     CDS             4780..5232
                     /gene="phnG"
                     /locus_tag="N7A07_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU2476483.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5229..5813
                     /gene="phnH"
                     /locus_tag="N7A07_001805"
     CDS             5229..5813
                     /gene="phnH"
                     /locus_tag="N7A07_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU2476484.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5813..6877
                     /gene="phnI"
                     /locus_tag="N7A07_001806"
     CDS             5813..6877
                     /gene="phnI"
                     /locus_tag="N7A07_001806"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU2476485.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6870..7715
                     /gene="phnJ"
                     /locus_tag="N7A07_001807"
     CDS             6870..7715
                     /gene="phnJ"
                     /locus_tag="N7A07_001807"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU2476486.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7712..8470
                     /gene="phnK"
                     /locus_tag="N7A07_001808"
     CDS             7712..8470
                     /gene="phnK"
                     /locus_tag="N7A07_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU2476487.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8581..9261
                     /gene="phnL"
                     /locus_tag="N7A07_001809"
     CDS             8581..9261
                     /gene="phnL"
                     /locus_tag="N7A07_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU2476488.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9258..10394
                     /gene="phnM"
                     /locus_tag="N7A07_001810"
     CDS             9258..10394
                     /gene="phnM"
                     /locus_tag="N7A07_001810"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU2476489.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10394..10951
                     /gene="phnN"
                     /locus_tag="N7A07_001811"
     CDS             10394..10951
                     /gene="phnN"
                     /locus_tag="N7A07_001811"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU2476490.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10938..11373
                     /gene="phnO"
                     /locus_tag="N7A07_001812"
                     /pseudo
     CDS             10938..11373
                     /gene="phnO"
                     /locus_tag="N7A07_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11375..12133
                     /gene="phnP"
                     /locus_tag="N7A07_001813"
     CDS             11375..12133
                     /gene="phnP"
                     /locus_tag="N7A07_001813"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU2476491.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12210..12539)
                     /gene="yjdP"
                     /locus_tag="N7A07_001814"
     CDS             complement(12210..12539)
                     /gene="yjdP"
                     /locus_tag="N7A07_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU2476492.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12608..14878)
                     /locus_tag="N7A07_001815"
     CDS             complement(12608..14878)
                     /locus_tag="N7A07_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2476493.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            14992..16512
                     /locus_tag="N7A07_001816"
     CDS             14992..16512
                     /locus_tag="N7A07_001816"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2476494.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16509..17483
                     /locus_tag="N7A07_001817"
     CDS             16509..17483
                     /locus_tag="N7A07_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU2476495.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGSNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17502..18443
                     /locus_tag="N7A07_001818"
     CDS             17502..18443
                     /locus_tag="N7A07_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2476496.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18498..19181
                     /locus_tag="N7A07_001819"
     CDS             18498..19181
                     /locus_tag="N7A07_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HGU2476497.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19196..20056
                     /locus_tag="N7A07_001820"
     CDS             19196..20056
                     /locus_tag="N7A07_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HGU2476498.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20053..21054
                     /locus_tag="N7A07_001821"
     CDS             20053..21054
                     /locus_tag="N7A07_001821"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU2476499.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHITHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21051..21758)
                     /locus_tag="N7A07_001822"
     CDS             complement(21051..21758)
                     /locus_tag="N7A07_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU2476500.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22049..24034
                     /gene="yjcS"
                     /locus_tag="N7A07_001823"
     CDS             22049..24034
                     /gene="yjcS"
                     /locus_tag="N7A07_001823"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU2476501.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5349 a         7010 c         6736 g         4945 t
ORIGIN      
        1 tttgcgtttc ctttcaaatg aaaacgatcg tcgtctaaaa tcagcagtac ccccgacaaa
       61 ctcagggatt ttgtgtataa ttgcggcctt tttcggcaat ctgccgtttt ttggcgcttt
      121 tgccctgctg acttttgagg aaatccacat gtcattacca cactgcccaa aatgcaactc
      181 cgaatacact tacgaagata acggcatgta catctgcccg gaatgtgcct acgaatggaa
      241 cgacgcagaa cctgcacagg aaagcgacga gctgatcgtt aaagatgcta acggcaatct
      301 gctggctgac ggcgacagcg ttaccatcat taaagatctg aaggtgaaag gtagctcttc
      361 gatgctgaaa attggcacca aagtgaaaaa catccgcctg gttgaaggcg accataacat
      421 cgattgcaaa atcgacggtt ttggtccgat gaaactgaaa tctgagtttg tgaaaaagaa
      481 ctgattgtat tgtgatcggt aagccggata aggcgtttac gccgcatccg gcaacagtgc
      541 cagatgcctg atgcgacgct cgcgcgtctt atcaggccta caaattcccg caccctccgt
      601 aggccggata aggcgtttac gccgcatccg gcaacagtgc cgactgcctg atgcgacgct
      661 cgcgcgtctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct
      721 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      781 ctccgacgcg attgcctatt atcaacgtac gttgggcgct gaactgctct ataaaatcag
      841 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      901 atttcccgat accgccatcg cccacgccaa tgtgcgcatt gccggaagcg atatcatgat
      961 gagcgatgct ataccgtcag gaaaagccag ctactccggt tttacgctgg tgctcgattc
     1021 gcaacaggtc gaagaaggaa aacgctggtt tgataacctt gccgctaacg gaaaaatcga
     1081 aatggcctgg caggaaactt tctgggcgca tggctttggc aaagtcaccg ataaatttgg
     1141 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataatccg ccgggaggcc
     1201 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1261 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1321 gcaaacgatt atctgcgtcg aacaactgag caaaaccttc aaccagcatc aggcgctgca
     1381 cgcggttgat ctgaatatcc atcatggtga aatggtggct ctgcttgggc cgtccggttc
     1441 cggcaaatcc acccttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1501 tagccatatc gagctgctgg gccgcactgt ccagcgcgaa ggccgcctgg cccgcgatat
     1561 ccgcaaaagc cgcgccaaca ccggctacat cttccaacaa ttcaacctgg tgaatcgcct
     1621 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1681 ttttagttgg ttcacccgcg agcagaaaca gcgcgcatta caggcgctga cccgcgtcgg
     1741 tatggcgcac tttgcgtatc agcgtgtttc cacgctctcc ggcggccagc agcagcgtgt
     1801 ggcgattgcc cgcgcgctga tgcagcaggc gaaagtgatt ctcgctgacg aaccgatcgc
     1861 ctcgctggac ccggaatcgg cgcgcatcgt gatggacacc ctgcgcgaca tcaaccagaa
     1921 cgacggcatc accgtggtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     1981 acgcatcgtc gccctgcgcc aggggcacgt cttctacgac ggcagcagcc aacagtttga
     2041 taacgaacgt tttgaccatc tctaccgcag catcaaccgc gtcgaagaga acgcgaaagc
     2101 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2161 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2221 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg
     2281 gacgccgttt ttacaggata tggagaagaa gctgggcgtg aaggtcaacg ccttctttgc
     2341 cccggactat gcgggcatta tccagggaat gcgtttcaac aaagtggata tcgcctggta
     2401 cggcaacctg tcggcgatgg aagcagtgga tcgcgccaac ggccaagtct tcgctcagac
     2461 cgtcgcggcg gatggatcgc cgggttactg gagcgtgttg atcgtcaaca aagacagccc
     2521 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gtaatggcga
     2581 tcctaactcc acctctggct tcctcgtccc cggctactac gtcttctcca aaaacaatat
     2641 ctccgccagt gacttcaagc gcaccgtcaa cgccgggcat gaaaccaacg cgctggccgt
     2701 cgccaacaag caggtggatg tggcgaccaa caacaccgaa aacctcgaca agctgaaaac
     2761 ctccgcgccg gagaagctga aagaactgaa agtgatctgg aagtcaccgc tgatcccagg
     2821 cgatccgatc gtctggcgta aaaacctttc cgaaaccacc aaagacaaga tctacgactt
     2881 ctttatgaat tacggcaaga cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     2941 gccgttccgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cactgtttaa
     3001 agagatgcaa agcgtgaaag gcaataaagg gctgaatgag caggacaagc tggcaaaaac
     3061 caccgagatt caagcgcagc tggatgacct ggaccgcctg aacaacgcgt taagcgcgat
     3121 gagttcggta agtaaagcga tgcagtaaat cgtaggtcgg ataagacgcc ccggtgtcgc
     3181 atccgacaat gtgcaggcgt tgatgccgga tgcggtgcaa gcaccttatc cggcctacag
     3241 accggagcca aacatgcaaa ccatcaccat cgccccaccc aagcgcagct ggttctcgct
     3301 tctgagctgg gccgttgtac tcgccgtgtt ggtcgtctcg tggcagggcg cggaaatggc
     3361 cccgctcacg ctgatcaaag acggcggcaa catggcgacc ttcgccgccg acttcttccc
     3421 gcccgatttc agccagtggc aggattacct caccgaaatg ctcgtcacgc tgcaaatcgc
     3481 cgtctggggc accgcgctgg cggtggttct ctccatcccc ttcggcctga tgagcgccga
     3541 aaacctggtg ccgtggtggg tttaccagcc cgtgcgccgc ctgatggacg cctgccgcgc
     3601 cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc gtcggcctcg gcccattcgc
     3661 tggtgtgctg gcgctgttta tccacaccac cggtgtgctc tccaaactgc tttccgaagc
     3721 ggtggaagcg attgaacccg gcccggtgga aggcattcgc gccaccggcg ccaacaagct
     3781 cgaagagatc ctctacggcg tgctgccgca ggtgatgccg ctgctgatct cctactccct
     3841 ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc ggcatggtcg gcgcgggcgg
     3901 gatcggcgtc acgctgtggg aagcgattcg cggtttccag ttccagcaaa cctgcgccct
     3961 gatggtgctg attatcgtca cggtcagcct gctggatttc ctctctcaac ggttgcgtaa
     4021 gcactttatc tgattagcga ggcattgata gctatgcact tgtctacaca tccgaccagc
     4081 tacccaacac gctatcagga gatagccgca aaacttgagc aggagcttcg tcaacactac
     4141 cgctgcggcg actatcttcc cgccgagcag cagctggcgg cgcgctttga ggtaaatcgc
     4201 cacaccctgc gccgcgccat cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag
     4261 ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt
     4321 tttagccaga atctgctgga tcagggcagc catcccacca gcgaaaaact gctgtctgtg
     4381 ctgcgcccgg cctccggtca cgtcgctgac gcgctgggga taaccgaggg ggagaacgtc
     4441 atccatctgc gcaccctgcg ccgggtcaac ggcatcgcgc tctgtttaat cgaccactac
     4501 ttcgcggacc tcaccctctg gccgacgctg caacgcttcg acagcggctc gctgcacgat
     4561 tttctgcgcg agcaaacggg gattgcactg cgccgcagcc agacgcggat cagcgcccgc
     4621 cgcgcccagg ccaaagagtg ccagcgtctt gaaattccga atatgtcccc gctgctgtgc
     4681 gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg cggagtactc cgtcagcctg
     4741 acgcgcgccg acatgattga attcactatg gagcactgaa tgcacgcaga taccgcgacc
     4801 cgccagcact ggatgtccgt actggcacac agccaaccgg cagaactggc agcccgcctg
     4861 aacgcgctga acatcaccgc caactatgag gtgatccgcg ccgctgaaac tggcctggta
     4921 cagattcagg cgcggatggg cggcaccggc gaacgctttt ttgccggcga cgccacgctg
     4981 acccgcgccg ccgtacgtct gactgacggc accctcggct acagctgggt gctggggcgc
     5041 gacaaacagc acgccgaacg ctgcgcgctg attgacgcgc tgatgcagca aacccgccat
     5101 tttcaaaact tatcagaaac gcttattgcc ccgctggacg ctgaccgtat ggcacgcatt
     5161 gccgcacgcc aggccgaagt gaacgccagc cgggtcgact tctttacgat ggttcgcgga
     5221 gacaacgcat gaccctggaa accgctttta tgcttcccgt gcaggatgcc cagcacagtt
     5281 ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat tgtcgccctg catcagctca
     5341 aacgcggctg gcagccgctg aatatcgcca ccaccagcgt gctgctgacg ctggccgata
     5401 acgacacgcc ggtgtggctt gccgcgccat tgagcaacga tatcgtcagc cagagcctgc
     5461 gttttcatac caacgcgccg ctggtcagcc agccggaaca ggcgaccttc gcggtaacgg
     5521 atgaggcgat ttccagcgaa cagcttaacg ccctttccac cggcaccgcc gttgcgccgg
     5581 aagcgggcgc aacgctgatt ttacaggtcg ccagcctgag cggcgggcgc atgctgcgtc
     5641 tcaccggcgc gggtattgcc gaagaacgaa tgatcgcccc gcagctgccg gagtgcattc
     5701 tgcacgaact caccgagcgc ccgcacccgt tcccgctcgg catcgacctg atcctgacct
     5761 gcggcgagcg cctgctggct gttccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt
     5821 tgccgtgaaa gggggcgaga aggcgatcga cgccgcccac gccctgcaag agagccgacg
     5881 ccggggcgat accgatttgc ctgaactgag cgtcgcccag attgaacagc agcttaatct
     5941 tgcggtggat cgcgtgatga ccgaaggcgg cattgccgac cgcgagctgg cggcgctggc
     6001 gctgaaacag gccagcggcg ataacgtcga agcaattttc ctgctgcgcg cctaccgcac
     6061 cacgctggcg aagctcgcgg taagcgagcc gctcgacacc acggagatgc gtctcgaacg
     6121 gcgtatttca gcggtttata aagatattcc cggcggccag ctgcttggcc caacctacga
     6181 ctacacccat cgcctgctcg atttcaccct gctggcaaac ggcgaagcgc cgacgctgac
     6241 caccgccgac agcgaacagc agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg
     6301 gctggcgaag tttgaagagg atagcggcgc acagccggat gacatcaccc gcacgccacc
     6361 ggtttacccc tgctcacgtt cttcccgcct gcaacagctc atgcgcggcg acgaaggcta
     6421 tttgctggcg ctggcctact ccacccaacg cggttacggg cgcaatcacc cgttcgcggg
     6481 tgaaatccgc agtggttaca tcgacgtgtc gattgtgccc gaagagctgg gatttgcggt
     6541 gaacgtcggc gaactgctga tgactgagtg tgaaatggtc aacggtttta tcgacccacc
     6601 ggatgaatct ccgcacttca cgcgcggcta cgggctggtg ttcggcatga gcgagcgcaa
     6661 agcgatggcg atggcgctgg tcgaccgcgc tctgcaagcc ccggagtacg gcgagcacgc
     6721 gacaggcccg gcgcaggatg aagagttcgt gctggcacat gccgacaacg tcgaagccgc
     6781 aggctttgtc tcgcacctca aactcccgca ctacgtcgat ttccaggccg aactggagct
     6841 actcaaacgt ctgcaacagg agcagaacca tggctaatct gagcggctac aactttgcct
     6901 acctcgacga gcagaccaaa cgcatgatcc gccgcgccat cttaaaagcg gtggcgatcc
     6961 ccggttatca ggtgccgttt ggcgggcgcg agatgccaat gccgtacggc tggggaaccg
     7021 gcggcattca gcttaccgcc agcgtgattg gcgaaagcga cgtgctgaag gtgattgacc
     7081 agggcgcgga tgacaccacc aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg
     7141 gggtaaacac caccgagcgc acggacgatg caacggtcat ccagacgcgt caccgcatcc
     7201 ccgaaacgcc gctgaccgaa gatcagatca ttattttcca ggtgccgatc cccgaaccgc
     7261 tgcgctttat cgagccgcgc gaaacggaaa cccgcaccat gcacgcgctg gaggaatacg
     7321 gcgtgatgca ggtgaagctg tatgaagata tcgcccgctt cggtcatatc gccaccacct
     7381 acgcctatcc ggtgaaggtg aacgggcgct acgtaatgga cccgtcgccg atcccgaaat
     7441 tcgataaccc gaaaatggac atgatgcccg ccctgcaact cttcggcgcc gggcgtgaga
     7501 agcgcatcta tgcggtgccg ccgtttaccc gcgtggaaag cctcgatttc gacgatcacc
     7561 cgttcaccgt tcagcagtgg gatgagccat gcgccatctg cggatcgacc cacagctatc
     7621 ttgatgaagt ggtgctggat gacgccggaa accgcatgtt tgtctgctcc gataccgatt
     7681 attgccgcca acagagcgag gcaaaaagcc aatgaatcaa ccgttacttt cggtcaataa
     7741 cctgacccac ctttacgcgc cgggcaaagg ctttagcgat gtctcttttg atttatggcc
     7801 gggggaagtg ctgggcattg tcggggaatc cggctccggg aagaccacgc tgctgaaatc
     7861 aatttccgcg cgtctgacgc cgcagcaggg ggagattcac tatgaaaacc gttccctcta
     7921 tggcatgagc gaggccgacc gccgtcgcct gctgcgcacc gaatggggcg tagtgcatca
     7981 gcatccgctc gacggcctgc gccgccaggt ctcggcaggc ggcaatatcg gcgagcggct
     8041 gatggcgacc ggggcgcgtc attacggcga tattcgcgcc accgcgcaga agtggctgga
     8101 agaggtggaa attcccgcca accggatcga cgacctgccg accacctttt ccggcggtat
     8161 gcagcagcgt ttgcagattg cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga
     8221 tgaaccgacc ggcgggctgg atgtgtcggt gcaggcccgc ctgctcgacc tgctgcgcgg
     8281 cctggtggtg gagctgaacc tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg
     8341 cctgctggcg gaccgtttgc tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac
     8401 cgaccgcgtg ctcgacgacc cgcatcatcc gtatacacag ctgctggtgt catcggtttt
     8461 gcagaattga gccggtgccg gatgcggcgt aaacgcctta tccggcctac aaatgcgctc
     8521 cccgtaggtc ggataagacg catcagcgtc gcatccgaca cccgaaccac gaggcgaaaa
     8581 atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc accagcaaaa cggcgtgcgc
     8641 ttgcccgtcc tcaaccgcgc ctcgctcacc gtcaacgcgg gcgaatgcgt ggtgctccac
     8701 ggccactccg gcagcggcaa atcaaccctg ctgcgctcgc tgtacgccaa ctatctgccc
     8761 gatgaagggc aaatccagat caaacacggt gacgagtggg tagacctggt cactgcgcca
     8821 gcgcgcaaag tggtggaaat ccgcaaaacc accgttggct gggtgagcca gtttctgcgc
     8881 gttatcccgc gcatttcggc gctggaagta gtaatgcagc cgctgctcga taccggcgta
     8941 ccgcgtgaag cctgcgccgc taaagccgcg cgtctgctca cccgtctgaa cgtgccggaa
     9001 cgcctgtggc gccttgcgcc atcgaccttc tccggcggcg aacagcagcg cgtcaacatc
     9061 gcccgtggct ttatcgtcga ctaccccatt ctgctgctcg acgaacctac cgcctcgctg
     9121 gacgccaaaa acagcgccgc cgtagtcgaa ctgatccgag aagccaaagc ccgcggcgca
     9181 gccatcgtcg gcattttcca tgacgaagcc gtacgtaatg acgtcgccga ccgcctgcac
     9241 ccaatgggag cctcttcatg attatcaata acgtcaaact ggtgctggaa aacgaagtgg
     9301 taaacggttc gctggagatg caggacggcg aaatccgcgc ctttgccgaa agccagagcc
     9361 gcctgccgga ggcgatggac ggcgaaggcg gctggctgct gccggggctg attgagctgc
     9421 atacagacaa cctcgataaa ttcttcaccc cgcgcccgaa agttgactgg cccgcccact
     9481 cggcgatgag cagccatgac gcgctgatgg tggcgagcgg catcaccacc gtactggacg
     9541 ccgtggcgat tggcgacgtg cgagacggcg gcgatcggct ggagaatctg gagaagatga
     9601 tcaacgccat cgaagagacg cagaaacgcg gcgtcaaccg cgccgagcac cgcctgcacc
     9661 tgcgctgcga gctgccgcat aacaccacgc tgccgctgtt tgaaaagctg gtgcagcgcg
     9721 agccggtgac gctggtgtcg ctgatggatc actcgccggg ccagcgccag ttcgccaacc
     9781 gcgagaagta tcgcgaatat tatcagggca aatactcgct caccgatgcg cagatgcagc
     9841 agtatgaaga ggaacagctg gcgcttgccg cacgctggtc gcagccgaat cgcgaatcca
     9901 tcgccgcaat gtgccgcgcg cgacaaattg cgctcgccag ccacgatgac gcaacgcacg
     9961 cccacgttgc cgaatctcac cagcttggca gcgtgatcgc cgaatttccc accacgtttg
    10021 aagcagcgga agcctcgcgc aagcatggca tgaacgtgct gatgggcgcg ccaaatatcg
    10081 tgcgtggcgg ttcgcactcc ggcaacgtgg cggccagtga actggcacag cttggcctgc
    10141 tggatatcct ctcttccgac tactaccccg ccagcctgct ggatgcggcg ttccgcgtcg
    10201 ccgatgacca gagcaaccgc tttacgctgc cgcaggcggt gaagctggtg accaaaaatc
    10261 cggcgcaggc gcttaatctc caggatcgcg gggtgattgg cgagggcaaa cgcgccgacc
    10321 tggtgctggc gcatcgcaag ggcaatcaca ttcatatcga ccacgtctgg cgtcagggta
    10381 aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg gggccgtccg gctccgggaa
    10441 agatagcctg ctggcggaac tccgcctgcg ggaacaaact cagctactgg tggcgcatcg
    10501 ctacatcacg cgcgacgcca gcgccggaag cgaaaaccat atcgccctga gcgagcagga
    10561 attttttacc cgcgcggggc aaaatctgct ggccttaagc tggcacgcta acggtctgta
    10621 ttatggcgtc ggcgtcgaga ttgacctctg gctgcacgct ggattcgacg tgctggtcaa
    10681 cggctcacgc gcccatctgc cgcaggcgcg ggcgcgctat caatcggcgc tgctgcccat
    10741 ttgcttacag gtttcgccag agatcctgcg ccagcgcctg gaaaaccgtg gccgcgaaaa
    10801 cgccagtgaa attaacgccc ggctggcgcg cgccgcccgc tatactccac aggattgcca
    10861 tacgctcaat aatgacggca gcctgcgcca gtcggtcgac acgctgctga cgctaatcca
    10921 tcagaaggag aaacaccatg cctgcttgtg agctttcgcc cggccacgca gtacgacacc
    10981 gacgcggttt acgcgttgat ttgcgagcta aaacaggcgg agtttgacca ccacgcgttt
    11041 cgcgtgggct ttaacgccaa cctgcgcgac ccgaacatgc gctaccatct ggcgctgctg
    11101 gatggcgaag ttgtcggtat gatcggcctg catttgcagt ttcatctgca tcatgtcaac
    11161 tggatcggcg aaattcagga gttggtggta atgccgcagg cgcgcggtct gaacgtcggc
    11221 agtaagttac tggcgtgggc agaagaagaa gcccgccagg ccggggccga aatgaccgaa
    11281 ctttcgacca acgtgaagcg ccacgacgcg caccgtttct atctgcgcga aggctacgag
    11341 cagagccact tccgcttcac caaggtgctg taacatgagc ctgaccctca cgctcaccgg
    11401 ctccggcggc gcacagggcg ttccggcatg gggctgcgag tgtgcggcct gcgccagagc
    11461 gcggcgctcg ccgcactatc gccgccagcc atgcagcggc gtagtgaagt ttaacgaggc
    11521 gatcaccctg atcgacgccg ggcggcacga tctcgccgat cgctggtcgc ccggatcgtt
    11581 ccagcagttt ttgctgacgc actaccatat ggatcacgtg caggggctat tcccgctgcg
    11641 ctggggcgtg ggcgatccga tcccggttta cggcccaccg gatgaacagg gctgcgacga
    11701 tctgtttaaa catccgggcc tgcttgattt cagccacacg gtggaaccgt ttgtggtgtt
    11761 tgatttgcag gggttacagg tcacacccct gccgctcaac cactcaaaac tgaccttcgg
    11821 ttatctgctg gaaacggcgc acagccgggt ggcgtggctg tctgacaccg ctggcttgcc
    11881 ggaaaaaacg ctgaaatttt tacgcaataa tcagccgcag gtaatggtga ttgattgcag
    11941 ccacccgccg cgcgaggatg caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct
    12001 gaatcaggtt atccgctcgc cgcgcgtggt tctgacccat atcagccacc agtttgatgc
    12061 gtggctgatg gaaaacgcac tgccgtcggg gtttgaggcg gggtttgatg ggatggagat
    12121 tggggtggcg tgatgagagg gaatgtgcgt gctggccccc tcaccctaac cctctcccca
    12181 gaggggcgat gcctgcccca tacccaacat cattgcccat actcatcttc cattctccgc
    12241 tcttcatctt ctagctgccg acgctcctga ttaagctggt gctggcgatc gtccagctgc
    12301 ctgcgacgat cttcaaactg gcgacggcgg tcgtcatatt gtctgcgccg atcgtcgctc
    12361 acttcacgct gccagccgtc gtcgcgcgaa tcttcataat ctcgcccacg gtcagggtta
    12421 gaagcgtcgt taatcgcctg ctgaatattg ccaatggtgt catcgataat atcggcctgg
    12481 gccggtacgg tggtcagcgt gagcagggtg aaaagaaaaa gcgtggagtg gcgtttcata
    12541 aactcggcct cgcaacaaag ttaatacaag gtaggcaaag gtgcctgtta cgagtagagg
    12601 aggattctca attctcttgc cccagcgcct gcgccaactg cgcacgggta aagggtttgc
    12661 gcaacagcgc cacgtctggc agcgcggggt tatggctggg gcgtaaatcc tgaccgctga
    12721 tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact tctgcgccgc
    12781 tcatgccgcc ggggagcatt aaatcgctga taaaaatatc gatttctgct gatgccgcca
    12841 gcagatccag cgcctgttcg ccgctgcttg cttccagcgt caaatagccc agcaggtgca
    12901 actgttcgca gatggtctgg cgcaccgcgg cttcatcttc cagcactaac accagtttat
    12961 cgccgctgac agaagcctgt tctactgccg ctggcgatag gtttttcgcc gccgtcagcg
    13021 cgcggggaag ctgtaatctg accgttgtcc cctgccccgg cgcgctttca atttcaacgc
    13081 gcccaccgga ctggcgcaca aaaccataga ccatcgacaa acccagcccg ctgccgctgc
    13141 ccgtctgttt ggtggtaaag aatggctcaa atacctggct tttcaccgcc tgcgacatac
    13201 cgcagccatg atcgatgacc tccagtgcca ccatgtcctg acgctgccca ctgctgcgag
    13261 taacgcgctg attccaggta cgaattttga tgattcccgt ctgcccttcc atcgcgtcac
    13321 gggcgttcat caccaggttg atgatggcat tttccagttg gctgatgtca atccacgcgg
    13381 gccacgccgg agactgcgct tcaatctcca gcgccagcgt tgccggtaag gagtggcgca
    13441 ttaattcgct taactcttcc agcaatgttt tcagttcaac ggcttgcgga tgtaaggact
    13501 gcttgcggga aaacgccagc agtcgttgtg tcagtagcgc cccacgttcg gcagcttgca
    13561 gcgcccgctg tattcgcggc gcgtctggcg agtccggatt caccagctcc aggctgccaa
    13621 taatcaccgc cagcagatta ttaaaatcat gggccagccc gcccgtcagt tgcccgacag
    13681 ctttcagttt ctggctgtgt aatagcgcct cttccaaccc ctgccgctca gtttgggcaa
    13741 tctccatctg cgtggtcttc tcttttagca gccgtgaggt tcgttcgagt gacgcggtat
    13801 tgcgggcaaa aacgttaaag gcgcgcgcca gttcacccaa ctcatcacgc cgctgtaacg
    13861 ccggaaaagt gacgtccgct tcgccgtgcg ccaggcggct catggcgcgg gaaatcgccg
    13921 tcaggttagt gccgaggttg cgatagatat accagccggc aaacgcggtg atcaccaccg
    13981 ctaacagggc gaaaatcatg atcaccctct cgccggagcg caaatcctgg taactttgcg
    14041 ccacacgctg accggagagc gtcgccacct gttcggcgta acggttgatg tcgccattga
    14101 ggatcgccac cagcgctttg atgtgaaaca tataccagct gattgccaga tcgctttgct
    14161 ccagagcggc agaaagcggc gcgagtttac gcatttcatc attgaaatca ttgaaatcat
    14221 tgagtaacgt attaaccaat ggcgtatcgc tgtgggtcgg caacgcagac attaccccga
    14281 ccagttgatg aataatcgcc tgcggcgcgg gggtggcaat agcggcaaca atcagtcggt
    14341 tcatctcgtt aagcaaaata tcatcttgcg cgtgagtgac tttttgcaga tgccgcagat
    14401 aacttaagtt ttgatacagc gaacttaaca gcgcgttgcg ctccagatgc cgcctttgcc
    14461 cgcgctccag cataccgccg acgctggttt gcagttcgtt gctacgcgtg atgattcgcg
    14521 tcaccagccc ggcttcctgc tgcgctaaag gcgcgttcgc cagttgtgca agcgagcttt
    14581 ttaatgcccg ctgtgtctcc tgcaaccgct cggcttcgcc tttatattcc agcgctccca
    14641 ccacctgcga caatcgcacc gccgccgttg ccacgtttgc cgtgtcgcgc gccagattca
    14701 ggctgccggt catatcatcc accgtttgct gctgcaccag ctcctgcatc tggctggcat
    14761 gacgaaagcc cattaccgcc acgccgctga ccatcaacgt caccgccacc accagcaaat
    14821 tcaaaagcag caatcgcccg cgcgcgctgg caaaaaagtg acgtccgggg tgcgccatat
    14881 cacgctccgt gaagaaaaat cgcaatatga caaatatgaa cggcttctga cattttgcat
    14941 ttactttcct gtgatggagt gctgatatcg acaacctcgc aggagtccac catgaacagc
    15001 acgccagttc tggagatgcg caatattgcc aaagccttcg gcaaatttta tgcactcaaa
    15061 ggggtggatt tgacggtcta ccctggcgag atccatgccc tgatggggga aaacggcgcg
    15121 ggaaaaagca cgctgatgaa ggtgctggcg ggggcgtata ccgccaccag cggcgagatt
    15181 ctcatagacg gcaagccctt tcacattcgc acgccaaaag atgccttaag cgccggtatt
    15241 acgctgattt atcaggagat gcagctggca ccgaatctgt cggtggcaga aaatattttt
    15301 ctcggcagcg agctttccca cggcgggctg gtgcagcgta aagagatgct agtgcaggcg
    15361 caaaaagtga tcgaccgcct cggcgcgcag tttaacgcca gcgataaggt catgacgctg
    15421 accattgccg agcaacagca ggtcgaaatc gcccgcgcac tacatcgcaa cagccgcatt
    15481 ctggtgatgg acgaacccac cgctgccctc tcctcccgcg aaactcaccg cctgtttgaa
    15541 ctgattatgc ggctgcgcga tgaagggatg gcgattatct acattagcca ccgcatggcg
    15601 gaagtgtatg agctttccga tcgcgtcagc gtgctacgcg acgggcaata cgttggcagc
    15661 ctgacccgcg ataacctcaa tgccggggag ctggtgcgga tgatggtcgg caggccactg
    15721 agcgatctgt tcaataaaga gcgcgatatc ccgctcggta aagcccgcct gaatgttcac
    15781 cacctgaccg acggcggcaa agtccagccg agtagcctgc tggtgcgttc cggcgaaatt
    15841 gttggcctcg ccggactggt gggtgccgga cgttccgaac tggcgcagtt gatcttcggc
    15901 gtgcggaaag cgacaggcgg aatgattgaa gtcgatggtg aaccggtggt gatccactcc
    15961 ccgcgcgaag ccatcgatct tggcattggt tttctcaccg aaaaccgcaa agaacaaggc
    16021 ttattccttg aaatggcagc agccgaaaac atcaccatgg caaccctgga gcgcgatgcc
    16081 cgctggggaa tgctcaatcg caaaaaagcg caaaccattt ccgatgacgc cattaagttg
    16141 ctcaacattc gcgtgcctca tgcccaggta cgcgcgggcg ggctttccgg cggcaatcag
    16201 caaaaactgt tgatctcccg ctgggtggcg attggcccgc gcattttact gctcgatgaa
    16261 cccgcccgcg gcgtggacgt tggcgccaaa agtgagatct accggatcat gaacgagatg
    16321 gcgcgcaagg gcgtggcgat cctgatgatc tccagcgaac tgccggagat agtgggaatg
    16381 agcgatcgcg tctatgtgat gcgcgaaggc agcattgccg gtgagttaaa cggcaaaaac
    16441 atcacccagg aaaacattat gaccttagcg actggcgtca acgacgccca ttcccaggcg
    16501 gtaacctcat gacgacctct gcaaaaacct ctgccaaaaa aatgctgatg agcgatctga
    16561 tgcaaacggt cggcatcttg cccattttga ttctgatcgt ggcggtattt ggctttatca
    16621 cgccaaactt ttttaccgaa agcaacctgc tgaatattac ccgccaggca tcgatcaaca
    16681 ttgttctggc ggcggggatg accttcatca ttttgaccgg cgggatcgat ctttccgtgg
    16741 gttcgatcct cggcaccaca gcggtggcgg cgatggtcgt ctcccttagc cctgagttcg
    16801 ccatgctgtc gatcccggca gcgctgatgc tcggcctgct gctggggctg ttcaacggcg
    16861 cgctggtggc ctttgccgga ttgccaccgt ttatcgttac gcttggcacc tatacggcgc
    16921 tgcgcggggc ggcgtatctg ctggcggacg gcaccacggt tatcaactcc aacattaatt
    16981 tcgagtggat aggcaacaac tatctcgggc cggtcccatg gctggtggtg attgcgctgg
    17041 cggtgatcgt ggtgtgctgg tttattctgc gccgcaccac gcttggcgtt catatctacg
    17101 ccgtcggcag caatatgcag gcggcgcgtc ttaccggcat taaggtctgg ctggtgctgc
    17161 tgtttgttta tggcatgagc ggcctgcttt ctgggcttgg tgggattatg agcgcctcac
    17221 ggctgtacag cgccaacggc aacttaggca tgggttacga actggatgcc attgcagcgg
    17281 tgatcctcgg cggcaccagt tttgtcggcg gcatcggcac catcaccggc acgctggtcg
    17341 gcgcgctaat tatcgccacg ctcaacaatg gcatgacctt aatgggcgtc tcttacttct
    17401 ggcaactggt gatcaaaggg gcgatgatca tcattgcggt gctgatcgac aaataccgta
    17461 cccgacacca tcaaagtgca taaacacatg aggaatacag catgagattg aagcccttag
    17521 tgaccgcgct ctgtgcaggc gcgctgctgg ccgcagcacc gttcgcccag gcgaaagaac
    17581 tgaaatcaat aggcgtaacg gtgggcgatc tggcgaaccc gttcttcgtg cagatcacca
    17641 aaggcgcgga gctggaagca cgcaaactgg caggcgataa cgtcaaagtg acgctggttt
    17701 ccagcggcta cgatctgggt cagcaggtgt cgcagataga caactttatc gccgctaacg
    17761 tcgatatgat tatcctcaac gctgccgatt caaaagggat cggcccggcg gtgaaacggg
    17821 cgaaagatgc cgggatcgtg gtcgtcgcgg ttgacgtagc ggcagaaggt gccgatgcca
    17881 ccatcacttc cgataacacc caggcaggcg aaatggcctg taaatacatc accgatcgcc
    17941 tgaaaggcaa aggcaacgtg gtgatcatca acggcccgcc ggtatccgcc gtgcaaaacc
    18001 gcgtcgaagg atgccagact gaatttaaac gccatcctga catcaaagtg ctctctgaca
    18061 accagaacgc gaaaggcagt cgtgaaggcg ggctggaggt aatgacggga ctgctggcgg
    18121 caaatccgaa gatcgacggc gtatttgcga ttaacgatcc caccgcaatc ggtgccgatc
    18181 tggcagcaaa acaggcacaa cgtaacgagt tctttattgt cggtgtggac ggaagcccgg
    18241 acgccgaaga ggcgctcaag cgtgagaact cgttgtttgt cgctactccg gcgcaagatc
    18301 cccaggtgat ggcagcgaaa gcggtggaaa ttggctacga cattttgcag ggcaaacctg
    18361 cgccgaaaga gccggtgctg atcccggtga cgatgatcga taaaaacaac gtcggcaatt
    18421 acaaaggctg gacggtgaaa taagtccaca cgccgggcgg cgcagctgcc cggtcaccgc
    18481 taaggagaac gctttcgatg aaacgctccg ctataaacga cattctcggc catacacggc
    18541 aattcttttc gcaacatgac gtacatctgc cgccctttgc cagtttctcg cctgctcaat
    18601 ggcagcagct cgacaccgcc gcgtgggagg aggtttttga tcttaaactc ggctgggatg
    18661 ttaccgcttt tggtcgcaac aactttgcag cacacgggct gacgctgttt accctacgta
    18721 acggctcagc aaaaggaatg ccgtatgtga aatgttacgc cgagaagatc atgcatgtgc
    18781 gcgatgcgca ggtcacgcca atgcattttc actggcgtaa gcgtgaggac attatcaatc
    18841 gcggcggcgg taatttaatt gttgaactgt ggaatgccga tagtaacgag caaacggctg
    18901 atagtgacat tacggtggtg atagacggct gccgccagaa acatactgca ggctctcagt
    18961 tgcgcctgtc tcccggcgaa agtatctgcc tgccgcccgg cctgtatcac agcttttggg
    19021 cagaagccgg ttttggcgat gtgctggttg gcgaagtctc ttctgttaat gacgacgacc
    19081 acgataacca ctttttacag ccactggacc gctacaacct gattgacgaa gacgaacctg
    19141 cacagttggt attgtgtaac gaataccgcc agttccgctg aataaggata acgttatgcc
    19201 attgatttct ctcgctgaag ggctggcaca tgcccgcgaa catcactatt cgttaggtgc
    19261 gtttaatgtg ctggactcgc actttttacg cgccttgttt gctgctgcca gacaagaacg
    19321 ttcgcccttt attatcaaca ttgccgaagt acattttaaa tatatctcgc tggattccct
    19381 ggtcgagaca gtgaaatttg aagcggcgcg tcatgacatt ccggtggtgt taaatctcga
    19441 tcacggactg cattttgaat cggtggtgca agcgttgcgg ctgggattca gctcggtaat
    19501 gtttgatggc tccacgctgg ggtatgaaga aaatgtccgc cagacgcggg aagtggtgaa
    19561 aatgtgccat gccgttggcg tatcggtaga agctgaactg ggcgcggtgg gtggcgacga
    19621 aggcggtgca ctctatggtc atgccgatga atccctgttt accgatccgc aactggcgcg
    19681 tgattttgtc gaccgtaccg gcattgacgc gctggccgtg gcgattggca atgcacacgg
    19741 caaatatcgc ggcgaaccga aactcgattt cccgcgcctc gacgctatcc gccagcaggc
    19801 tgctatcccg ctggtattac acggcggctc agggattagc gatgccgatt tccgccgcgc
    19861 catcgaactg ggtatccaca aaatcaattt ctacactggc atgtcgcaag cggcgctggc
    19921 tgccgttgag gcccgaatgg cgcaccgtca gccgatgtat gacgagtttg ccgagttact
    19981 gctggcagtg gaagccgcca tcagcgatac ggttgcacaa cagatgcgta tttttggcag
    20041 tgcggggcag gcatgatgga gcgtaaaggg attatcgccg caggcaatat gttggttgac
    20101 cacgtccatc agattatcca gtggccagag cgcggctggc tggcggagat cacccacagc
    20161 gaacgctcca ccggcggtgc gccgcttaac gtcctgctga cgctggcgaa aatgcgcacc
    20221 gggctgccgt tacaggcggt tgggctaatc ggtcaggaca gcgacggcga ttacatcatg
    20281 gcgatgcttg agcaatatca cgtcaatcgt cagcatgtgc agcgcactac ctttgccccg
    20341 acgtcgatgt cgcaggtgat gacagaccca agcgggcagc ggacgttttt ccattcgccg
    20401 ggtgcaaaca gacttttgga tcttccggcg ttcgatcagc tcgataacac catgaagatt
    20461 ttccatctcg gctatttact gttgctcgac agcctggata tgccggacga tgtttacggc
    20521 acccgtagcg cacgcctgct ggcgcaaatg cgtgatactg gctttgaaac ctcactcgac
    20581 ctggtctccc gtaagggcga tccgcgttat cagccgctgg tgctgcccgc gttgcgccat
    20641 ctggattatc tggtaattaa cgaactggag gccggggagt ttagcgggct ggaaattcgg
    20701 ttacccaacg gcgaaccgca tatcacgcat attgctgccg ccgcgcgtga actgttagat
    20761 gcgggcgtgc gccagcgggt ggtgatccac tacccggaag gggcgtgggg agagacccca
    20821 gaacagggcg gcgtgtggat cccttcgcaa aagttagaac aacgggaaat tatcggcagc
    20881 gtcggcgcgg gcgatgcatt ttgtgccgga tttttatatg gctgccatga acgctggacg
    20941 cttaccgaga gcatcaaact ggcacacgcc tgcgcgcggg caagcctgtt atgcgccaat
    21001 gccatcgacg gggcgaaaac gctggaagag ctgcaaacag aaatgtcgga ttaatgcacg
    21061 tcggcagcga agacataccc cagaccgcgc agagttttaa tcaacatcgg ctgatgcggg
    21121 ttgagttcga ttttgcgtcg taagcgcatg attaatacgt cgatggtgcg atcaaacacc
    21181 tccacgctgt cgctgtgggt gaacgccagc aactgctcgc ggctcaatac ccgacgcgcg
    21241 ttttgcgcca gagccagtaa caagccatat tcgccctgag ttaactcgac atgttgctga
    21301 agcggattgt gcaactggca gcgcatggta tcgagatacc agccattgaa tgacagcccg
    21361 gcgcttttct cgtcattaac cgccagcgcg ccaacccgtc gcaacaccgc tttcagtcgt
    21421 gcgaccacca cccgcggggt gaacggtttg cagatgtaat catccgcgcc catttccagc
    21481 ccgaccacca catcagactc actgcccatg ccggtcaaca tcaccaccgg caatgtcgga
    21541 cgcagtttct ggatttgctg caataccctc aggccgttgg tatcgggtag catcatatcc
    21601 agcaccacca gcgcaatgtc aggatcgccc tcaatacgca atatggcctc ctgtccggta
    21661 tggcagactg aaaccgtaaa gacatgttca cttaacacgt cctgcaacag tgcgcagata
    21721 gccgtgtcat catccaccac cagcacgacg ggtttcatgg ttacactccg catcattcat
    21781 ctgattaatc attgtgcgta cttcttgtcg aagtgaaagc tgaaagtaat ataaataata
    21841 aaaatataag tcaggtcaca ttattgatga tagtaataaa tatgtcgtac ttttccctct
    21901 tcagcacaaa tgcctaataa aatggatcgt tccgctcaca cttccactta ttaaagtcgc
    21961 aatttcccaa ttatgataac gccagcttta aatgacgtat tttgcatgga ttacatattt
    22021 acgacataaa tgataaatgg agatttatat gaataactct cggttattcc gtttgagcag
    22081 gattgttatt gcgttaactg ccgccagcgg catgatggta aataccgcta acgcgaaaga
    22141 ggaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg ccaaatcatt
    22201 accgtttagc gatcgtcagg attttgacga tgcccagcgt ggatttatcg ccccgctgct
    22261 ggatgaaggt attctgcgtg atgcgaacgg taaagtttac taccgcgcag acgattacaa
    22321 atttgatatt aatgccgctg cgccggaaac cgtaaacccc agcctgtggc gtcagtcgca
    22381 gatcaacggc atttctggcc tgttcaaagt caccgataaa atgtatcagg tgcgcggcca
    22441 ggatatctct aacattacgt tcgttgaggg ggagaaaggc attattgtca tcgacccgct
    22501 ggtgacgccg cctgccgcaa aagccgcact tgacctttac ttccagcatc gtccgcaaaa
    22561 accgattgtt gccgttatct acactcacag ccacaccgac cactatggtg gcgtgaaagg
    22621 cattatctct gaagccgatg ttaaatccgg caaagttcag gtgattgccc ctgctggctt
    22681 tatggacgaa gccatcagcg aaaacgtgct ggcgggtaac atcatgagcc gccgtgcgct
    22741 ctactcttac ggtctgttac tgccgcacaa cgcgcaaggc aacgtgggta atggccttgg
    22801 cgtgacgctg gcaacgggcg acccgagcat tattgcaccg actaaaacta tcgtcagaac
    22861 tggcgagaag atgattatcg acggcctgga gtttgacttc ctgatgaccc caggtagcga
    22921 agcgccagcc gaaatgcact tctatattcc ggccctgaaa gccctgtgta ccgccgagaa
    22981 cgccacgcat accctgcaca acttctacac tctgcgcggc gcgaaaaccc gcgataccag
    23041 caagtggacc gagtacctga acgaaacgct ggatatgtgg ggtaacgacg cggaagtact
    23101 gtttatgccg cacacatggc cggtctgggg caataagcat atcaatgatt atattggtaa
    23161 atatcgcgat actatcacgt tcattcacga ccagaccctg cacctggcga accagggcta
    23221 caccatgaat gaaatcggcg acatgattaa gctgccgcct gcacttgcca ataactgggc
    23281 cagccgtggc tattacggtt ctgtcagcca caacgcccgc gcggtgtata acttctatct
    23341 tggctattac gacggtaacc cggctaacct gcatccgtat ggtcaggtag agatgggcaa
    23401 acgttacgtg aaagcgctgg gcggctctgc ccgtgtcatc aacctggcgc aagaagcgaa
    23461 taagcaaggt gattaccgct ggtcggcaga actactgaaa caggtgattg ccgccaaccc
    23521 gggtgaccag gtggcgaaga atctgcaagc gaataacttt gaacagctgg gctatcaggc
    23581 cgagtccgcc acatggcgcg gtttctacct gaccggcgcg aaagagctgc gcgaaggggt
    23641 gcataagttc agccacggca ccaccggttc cccggacacc attcgcggaa tgtcggtcga
    23701 aatgctgttc gactttatgg ccgttcgcct cgatagcgcg aaagctgcgg gtaaaaatat
    23761 cagcctgaac ttcaatatga gcaacggcga taacctcaac ctgacgctga acgatagcgt
    23821 gcttaactac cgtaaaacac tgcaaccgca agccgacgcc tctttctaca tcagccgtga
    23881 agatctgcac gccgtgctga ccggacaggc aaaaatggcg gatctggtga aagcgaagaa
    23941 agccaaaatt attggcaatg gcgcgaaact ggaagaaatt atcgcctgtc tggataattt
    24001 cgatttgtgg gtgaatatcg taaccccaaa ttaatgtcac
//
LOCUS       DBFIFS010000048        23852 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.048, whole genome shotgun sequence.
ACCESSION   DBFIFS010000048 DBFIFS010000000
VERSION     DBFIFS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.048"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            275..2947
                     /gene="rcsD"
                     /locus_tag="N7A07_001824"
     CDS             275..2947
                     /gene="rcsD"
                     /locus_tag="N7A07_001824"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU2476502.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2964..3614
                     /gene="rcsB"
                     /locus_tag="N7A07_001825"
     CDS             2964..3614
                     /gene="rcsB"
                     /locus_tag="N7A07_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU2476503.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3814..6663)
                     /gene="rcsC"
                     /locus_tag="N7A07_001826"
     CDS             complement(3814..6663)
                     /gene="rcsC"
                     /locus_tag="N7A07_001826"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU2476504.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6938..7714)
                     /gene="yfaP"
                     /locus_tag="N7A07_001827"
     CDS             complement(6938..7714)
                     /gene="yfaP"
                     /locus_tag="N7A07_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU2476505.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDSSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7719..9368)
                     /gene="yfaQ"
                     /locus_tag="N7A07_001828"
                     /pseudo
     CDS             complement(7719..9368)
                     /gene="yfaQ"
                     /locus_tag="N7A07_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            complement(9369..13884)
                     /locus_tag="N7A07_001829"
                     /pseudo
     CDS             complement(9369..13884)
                     /locus_tag="N7A07_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(13908..14531)
                     /gene="yfaT"
                     /locus_tag="N7A07_001830"
     CDS             complement(13908..14531)
                     /gene="yfaT"
                     /locus_tag="N7A07_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU2476506.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(14528..16156)
                     /gene="yfaA"
                     /locus_tag="N7A07_001831"
     CDS             complement(14528..16156)
                     /gene="yfaA"
                     /locus_tag="N7A07_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU2476507.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            complement(16364..18991)
                     /gene="gyrA"
                     /locus_tag="N7A07_001832"
     CDS             complement(16364..18991)
                     /gene="gyrA"
                     /locus_tag="N7A07_001832"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU2476508.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            19138..19860
                     /gene="ubiG"
                     /locus_tag="N7A07_001833"
     CDS             19138..19860
                     /gene="ubiG"
                     /locus_tag="N7A07_001833"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU2476509.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(20001..23753)
                     /gene="yfaL"
                     /locus_tag="N7A07_001834"
     CDS             complement(20001..23753)
                     /gene="yfaL"
                     /locus_tag="N7A07_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU2476510.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFPANGVAAAIDLCQGYNIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NIVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         5721 a         6497 c         5829 g         5805 t
ORIGIN      
        1 acagaaaaac tataacgctt gttaactatt tcacaaataa ttaacatcag cataatttcc
       61 agcaatcttt gtttatttgc aattattttc gttgggcttt ttgtaggtta tttgtacagc
      121 aaaatggcgc ttgtacatct atttccccca atgcaggatg ataaatatca cgggagaata
      181 gagaatcatc aatcaggtaa gagtctggaa tttcacactg taccctttat actgccctat
      241 cacttcgcga agttttaaca ggtcataaac acgaatgcgt cagaaagaga caacggccac
      301 gacccgcttt tcactcctac cggggagcat tacccgcttc tttttactgt tgatcattgt
      361 gttactggtg acgatgggtg taatggtaca aagcgccgtt aacgcctggc tgaaagataa
      421 aagttaccaa attgtcgaca ttacccacgc tatccaaaag cgcgtcgata cctggcgtta
      481 cgtgacctgg cagatctacg acaacattgc cgcgacgacc tccccctcct ccggcgaagg
      541 tttacaagag acgcgcctga aacaggatgt ctactatctg gaaaaaccgc gccgcaaaac
      601 ggaagcgtta atctttggct ctcacgacaa ctcaacgctt gagatgactc aacggatgtc
      661 cacctatctg gacacattgt ggggcgcaga aaatgtaccg tggtcaatgt attacctgaa
      721 tggtcaggat aacagtctgg tgctgatctc aaccctgccc ctcaaagatc tcacctccgg
      781 atttaaagaa tcgaccgtca gtgacattgt tgattcacgt cgtgcagaga tgttgcaaca
      841 ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg cgccgcctgg cctggcagaa
      901 cggtcattac tttaccttgc gtaccacgtt caaccagccg ggacatctgg caacggtcgt
      961 ggcttttgat ctgccgatta atgatttgat cccaccgggt atgccgctgg acagtttccg
     1021 ccttgagcca gacgcgacgg caacgggaaa caatgataat gagaaagaag gaacggatag
     1081 cgtcagtatc cactttaaca gtacgaagat tgaaatctcc tcggcactca actctaccga
     1141 tatgcgcctg gtctggcagg ttccttatgg caccttattg ctggatacgt tgcaaaacat
     1201 tctgctgcca ctgctgctga acatcggttt gctggcgctg gcgttatttg gctataccac
     1261 attccgccat ttctccagtc gcagtacaga aagtctaccc aacacggcgg tcaataacga
     1321 attgcgcatt ttacgggcaa tcaatgaaga gatagtctca ctgctgccgc tcggcctgct
     1381 ggttcacgat caggaatcga accgcactgt cataagtaac aaaattgccg atcatttgct
     1441 gccgcatttg aatctgcaaa acatcaccac catggcggaa cagcatcagg ggattattca
     1501 ggcgacgatc aataacgagc tgtatgagat ccgcatgttc cgcagccagg ttgcgccgcg
     1561 cacacaaatt ttcattattc gcgatcagga tcgcgaagtg ctggtaaaca agaaactcaa
     1621 gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg atgaccttta tgaaaaacat
     1681 tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag agcgcggcta aactcaacgc
     1741 cccggaaagc aaacaactgg cgaatcaggc agatgtgctg gtgcgattgg tcgatgaaat
     1801 acagttagcg aacatgcttg cggacgatag ctggaaaagt gagacggtgc tgttctccgt
     1861 gcaggattta attgatgaag ttgtgccttc agtgttgcct gccatcaagc gtaaaggtct
     1921 gcaactgctg attaacaatc atctgaaagc acacgatatg cgccgcggcg atcgcgatgc
     1981 attacgacgt attttgctgc tactgatgca atatgccgtg acctcaacgc aattgggaaa
     2041 aatcaccctt gaggttgatc aggatgagtc ctccgaagac cgcctgacgt tccgcattct
     2101 ggacaccgga gaaggcgtaa gcattcatga aatggataat ttgcacttcc cgtttatcaa
     2161 ccagacccaa aacgatcgct atggcaaggc ggacccgctg gcattctggc tgagcgatca
     2221 actggcacgt aaactgggcg gtcatttaaa catcaaaacg cgggatgggc ttggtacacg
     2281 ctactctgtg catatcaaaa tgctcgcagc tgacccggaa gttgaagagg aagaagagcg
     2341 tttactggat gatgtctgcg taatggtgga tgttacttcg gcagaaattc ggaatattgt
     2401 cactcgccag ttagaaaatt ggggtgcaac ctgtatcaca cccgatgaaa gattaattag
     2461 tcaagattat gatatctttt taacggataa tccgtctaat cttactgcct ctggcttgct
     2521 tttaagcgat gatgagtctg gcgtacggga aattgggcct ggtcaattgt gcgtcaactt
     2581 caatatgagc aacgctatgc aggaagcggt cttacaatta attgaagtgc aactggcgca
     2641 ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat gcgcaactcc atgccagcgg
     2701 ctattatgcg ctctttgtag acacagtacc ggatgatgtt aagaggctgt atactgaagc
     2761 agcaaccagt gactttgctg cgttagccca aacggctcat cgtcttaaag gcgtatttgc
     2821 catgctaaat ctggtacccg gcaagcagtt atgtgaaacg ctggaacatc tgattcgtga
     2881 gaaggatgtt ccaggaatag aaaaatacat cagcgacatt gacagttatg tcaagagctt
     2941 gctgtagcaa ggtagcctat tacatgaaca atatgaacgt aattattgcc gatgaccatc
     3001 cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat tgagtgggtg aatgttgtcg
     3061 gcgaatttga agactctaca gcactgatca acaacctgcc gaaactggat gcgcatgtgt
     3121 tgattaccga tctctccatg cctggcgata agtacggcga tggcattacc ttaattaagt
     3181 acatcaagcg ccatttccca agcctgtcga ttattgttct gaccatgaac aacaacccgg
     3241 cgattcttag tgcggtattg gatctggata tcgaagggat cgtgctgaaa caaggtgcac
     3301 cgaccgatct gccgaaagcc ctcgcagcgc tgcaaaaagg aaagaaattt accccggaaa
     3361 gcgtttctcg cctgttggaa aaaatcagtg ctggaggtta cggtgacaaa cgtctctcgc
     3421 caaaagagag tgaagttctg cgcctgtttg cggaaggctt cctggtgacc gagatcgcta
     3481 aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa gaaatctgcg atgatgaagc
     3541 tgggtgtcga gaacgatatc gccctgctga attatctctc ttcagtaacg ttaagtccgg
     3601 cagataaaga ctaatcatct gtaggccaga taagacgcgt tagtgtctta tctggcattt
     3661 gcaccgattg ccggatgcgg cgtaaacgcc ttatccgtcc tacgattccc attatttcaa
     3721 cagattacat taaagtaggc cagataagac gcgtcagcgt cgcatctggc atttgcactg
     3781 aatgccagat gcggcgtaaa cgccttatcc gtcctacgat tcccgcgatt tcctgaccct
     3841 ctcggcatat accgtcagcg tctgttttat aacatccagc gttactggct tcgacaggca
     3901 gctgtccata ccggactcca gacaccgctg cttctcttca gccaacgcat tagccgttac
     3961 tccgattacc ggcaacgtca gtcccaactg acgaatgcgt tgtgtcaagc ggtaaccatc
     4021 catatttggc atgttgacgt cgctaagcac gatatcaata tgattcttgc taagtacatt
     4081 aagcgcatcg acgccatcat tcgcggtttt acattgatag cccaacgatc ccaactgatc
     4141 tgccagcaaa cgccggttaa tcggatgatc atccacgacc agaatcatca tatcgtcatt
     4201 atcgctgacc gctttgtccg tcgacggcag agcgttagca ggatcgtcgc tctccatctc
     4261 gatcaaataa atacgcgcca acaatgccgg tagctcatgc ggagcagcca cactgtgtac
     4321 ccactcccct ggcgctttct ccagcggaat accaatatgt cgacgacaga aggtcactac
     4381 cgctctgccc tgccattttt tactcactac ctcgtcagtg attaacacat cttcgggagt
     4441 cggttcctgc ccttcgtatg ttgttacgac gatgccgctg cgctgcaaac tggtttccag
     4501 gaactgacag agcgacgcat tgcggaccgc cagccagcag cgtttaccac tcaacccttc
     4561 cacgcctttt ttctgcgggt actgagcgcc gtacaacgga atacgcacgg taaactggct
     4621 gcccattccc ggttctgaat ctaccgagat atcgccgtcc atcatgctga tcagtttttc
     4681 acaaatcgcc agacccagac cggtcccctg gaagtttcgc tgtacgcccg ttccgacctg
     4741 gaagaaggga tcaaacaagc gcaccacttc tttcgccgga atccccacgc cggtatcgcg
     4801 aacacggata gagagataat cgccatcagc gcggacatgc aaaactatac agccggtatc
     4861 ggtgaatttt atggcgttac tcaacaggtt ggagatgacc tgctgtaaac gcatcgggtc
     4921 gccatttaag gccactggca catccggttc aataaagcag tacaagccta actgcttgcg
     4981 taccaccagc ggtaaatagt tggcggtgat gtggttcatc acttcacgcg gtgaaaactc
     5041 acgcggttcg atcatcaact gttccgattc aatcttcgag aaatcgagaa tatcgctgat
     5101 aattttcaac aacaggctgg aagagttgtt cattgccgtc accagacgat cgacgccttt
     5161 cggtaactct ttggtttgca acagatccag gttaccgata atgccataca gcggcgttcg
     5221 cagctcatga ctgacggtgg caaggaacat cgattttgac tggctcgcct gttccgctgc
     5281 ttgtgccatc tcctgcaacg actcttccat cttcacgcgc gaagaaacat ccaccagcac
     5341 acaaatggcc acgttttcat tacgatagcg cgaatggacg aagctgattt gcagattggt
     5401 attgttgctg gtcaggacat caacaaaatt gacctgctgc ccacagataa tttgtgtcag
     5461 tcgttggcgg tcctcatgcg taagcatatt gagataggta tgcgccagtt cgttacttaa
     5521 aatattgacg ccatcagcgg tacgcaaaat gcagataccc actggcgcgg aggcgacaat
     5581 cttgcgattg aactgctcat gttcttccag tcgcagggcg tcgctttccg ccggaatgaa
     5641 aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca gctccggcaa gcacattcag
     5701 caaaattgcg ttaaggatca acatgcgaat gcgttccagc accttatcaa ccggcaccga
     5761 atacacgatg ctgagcgatg agggtggcag atttttcttc agcaccagct cccggaaccc
     5821 ttccgtatag ccaaaccagg agcgttcctg catccagcga ggatcggcct taattttgct
     5881 ttctggcccg gtaagcgaaa tcagggtatg accattttca tcaagaatgg taacccccat
     5941 cggcaacgta cccggtaaga aaaagttctc catccggatg gtctgctcga cacccaaaag
     6001 cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg taaaaatacc cgacgccagg
     6061 gcgcggacct tcgctgatcc agtagaggtt actgccgcta tcatcttgtg gtgcatttcg
     6121 atatttattg atgcgttcat gcaaagcttt caacgcggta tcgcgttcca ctggcatatc
     6181 acgcagaccg aaattggcca tgcagaggtt atcgctgccg attaaaaata cccggttgag
     6241 atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata aaccacgcca atgactccag
     6301 agaacctcgc caggtgttac tcattgctga acaatcggag tcggcaaaca gcggttcaaa
     6361 cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa agcacaccgt tttctgccga
     6421 taagcgattt tcggcgatgt acttcagctc tttcatcaca tcagaggtgc gttgaataaa
     6481 gcgctgagcc tgatcggaac tcagattaaa ttcctgacga atttccgatt ctcgctgatg
     6541 taacgcatta acgatgtaaa aaacggatga aaaagcaatc aacagccaga gcactaacgc
     6601 caatgctctg aacatgtagc gcgaggcttt cagggttgta cgaaaagaag caaggtattt
     6661 caaaggggcg aagctccgcc tcaggtgacc gatggagtgt ggttaaggta gcggtaaaag
     6721 cgtgttaccg caatgttctg tcaccattgc aataatgcgt atcgcttaga aaccattgcc
     6781 ttaaccagga aagccggagt aaatggacag ttctcagtac gaaaaataag aaatgaccaa
     6841 tctgccgacg cttgttaatg gttatatcct ggcgtcggca gatcgcccag cagtcttatc
     6901 tgaaaaaaga aagagccagg ttgtgtgaag ggatggatca ccagtcaaaa cttttcacca
     6961 gcgtcagctc gccagcatta cgcatcggta caataaatgt ttcctgtttc tcattgaccg
     7021 atccttcatc ggtgatcagc gtcagttggg cggtggttaa ttccgtttcg ctgcgcccac
     7081 catagtagtt gatatacacc tgatagcgcc cgtgaactgg tgctggcatg gcgaaaatct
     7141 cgggtccgta ccccgtcgtg acatccatat ccaatgcacc actgtttttc agcacggtgt
     7201 taccgtacca ggcgtgttcg ccatcgggcg tgacaacgtg aagatcgagg tcggtattgt
     7261 ccgtatccca cgagagaacc agccgtaaac gtgcacgaat cgttcctgta cccggcgttg
     7321 agtaaaactg cattttttgt cggctttgcc catccgggct gatgacctgc acgctgttgc
     7381 tgccttcagt gaaaatataa gggcgtgcaa atgaactatc ggattcaatt ctctgcggca
     7441 tactggcacc gttgaccacc agccggccct gctgaacttt acccgccgca gcataattct
     7501 ttattttgcc gcggatctgc gctgagatat tttgatcatc tgccatgttg acggacgacg
     7561 ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc ctctgccgga tgccagccag
     7621 agagcggtgc gtcaatctcg acttcctgca caccttcact gtgagcgaca ggcgaaagcg
     7681 ccaccagtaa caacggaaga aaaatctttc gcatagcatt agtccattaa taattgtcgg
     7741 gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat aaccatcaaa ggccaggtgc
     7801 agatactcat gggttaaatc cagccgatcc tgaagcgtaa agaagttacg aataaacagc
     7861 cgtttctgct gccgatcggt ataggggaaa cctgagacta aacgacagac cgcaaacacg
     7921 tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc gccactgcgg catttttttc
     7981 gccagccagg ctttcgcttt gggtaataac tgacaggttg tgcgtggcgc tccccagcgg
     8041 ctaaggctgt tgtcgggata agcaaacgcg agtatctggt cgtaacgctc tcccttcccc
     8101 gcctgggcca tggcttgccg ccaggaaagc gtcccttcgg tagcgcgact gccgtgataa
     8161 tgaaccggat cgcccgcata gatgagatcc tgcgtccaga cggtcatcgt tcgcgccccc
     8221 gtggtagctg gcgaagcaga aacacgctgc gtggcgctgc tgtcggggat ggtcaggcaa
     8281 tcgccttccc gattggcgtt ttgctgtaaa aaagtgcgta tcgccacagt catggctttc
     8341 gctgcctcag gtggcgtact tttcgcctcg cgatccagaa ctctggcaac gtactcttca
     8401 cgatcgagat gcgactgcaa tttcagtttg cccttttcgg tcagtaaggt cgtctcaccg
     8461 tggctgacaa acgtaatgtg gttaccattg gcaaacgtca cacgatagcg cccattaagt
     8521 acgccgggtt ttactgccgt cgtactcttc tccgctgtga ttttcttcag cggctagcgc
     8581 gcaaatagct ccacctctac acattgccca ctggcaactt gcgtcggcac tggcaaaacc
     8641 cgattaagca ccgtcgcata acgggtcagt accgttttgc tggtgccact gccagtcacc
     8701 cacagcggtg taccatcggt taaccagcca gcaaaaccac cctgacggat ttcctgttta
     8761 tcatcggcaa accagctcca ggttttcacc cgtacccgac taccgagcat agatgcgaca
     8821 ccgattttcg cctcgtccag caccacatcc agtaagactt cctgagcttt gttttgcgca
     8881 ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta ccgaggtttc tggcttcagc
     8941 gtagaaagag acgctaacca cgcaggtgcc tgacgctgtt gccagtattg cccccacaca
     9001 tctgccccaa tatgcaaccg ctgcggcgca aaatagagcc cacaggaacg taccagcgcg
     9061 gtatcgcgcg ttatgctctc cccagcctga caacaataaa cctcttccgg ggagtttccg
     9121 cgacactggt agggctgctc cggttgatgg gtatcctcca gccaggcgta aacatacagt
     9181 ttccataaac tccctaacgg cgtggtgagc gtgtcaggta atgctgagac tttggtcacg
     9241 cctgatgacg acagctgata cagttgatcg tgctgcgcac cgcgcagcgc cagttgtaac
     9301 ggcgtctcct ccgcaagcgt tggcagagta accaacgcca atagccagac gattctgcgc
     9361 cagttcactt atttcacctg catcccggtc cattcgctcc ctgccgcaac actttgctgt
     9421 gcaggtgcat aggaacgcac ataacgagca ggaggcagaa cgaattgccc tttttgcgag
     9481 aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt ctttcaccgg caccatatag
     9541 gccagttcgc ccatttcatt tcgcgctttt tccagcaatt gcccctgctg ttttccagcg
     9601 ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa cgtcggctcc cggcgggagc
     9661 ggtacttcca cctgaccata gcgcagaact gcatcctgct cgctggtaag cgtaatttca
     9721 tcgagataca gcgcatcgct gtcaatctca ttgctggtca ccggttgcag agtaaagctc
     9781 atctcttctt caccagggat aagccgatac aattggcgtt caacggtcac cggaatgtta
     9841 ctttgttgag ccgtttttgc cgcttcacgc cagcggacct gcacattttg cggcgataat
     9901 tcgtcaccaa aagagagaat gtccggcacg ccctgaccaa cccaacgcca gtattcgccc
     9961 cctccagtta acttatgttt ggcccatgcg cccgcaggcg caggcaacac aaccgaaggc
    10021 attgtcgcca tatatttcgc cagccagttc atggccagcg cgcgctcaat agtagattgc
    10081 tcagcggtca aaccacttaa aatagcagcc gcctgggtag cgtcgccccc gctattgagc
    10141 agcacaaccg tgtgtgccag cggctgattg ctgtttaccg ctttatccca cgcatattgc
    10201 tgaacctgtg gcatcaccgt ggacttcaac tgcgcttttt tcagcaacgt catggttaag
    10261 acgtttgcca ccgcatctgc cagtggagat tccggtgtat cgaggatcag gctgtcattg
    10321 atatcgcggg tgtcttcctc agagaaatct tcagttttag ttccgcgccg ggcgatagct
    10381 tcatccaacc ctttcaacaa cgttttgcac ggcagattca tctcctgtgc ccacgccagc
    10441 accagcgccc gatgcaataa cggcatgtta tctgcctgct cggcataact gtcgagcata
    10501 tgctgccagt attccggttg ttgcgttacg ccgagcgcct ggctggcctg ccagtcggcg
    10561 taccatgccc atgccgtaag gaaggcgtca ccattgccat cttcacccca ccaggtaaag
    10621 cgcgctccgg gccccgccag ttgcatcagc cgcagacggt tatcctgaat catctgacga
    10681 atgtcgttag cggcggcact ttgatgatcg gcaagcgaac gccaggcgag actgagcggg
    10741 atcagacggc taccggtgtt gatcaccccc cccccacggt tcatcgacta acgcatcaag
    10801 attgttgcgg aaaatctcct gcggcgtttc actactttgt agccggatat tgctcgcctg
    10861 ctcgggcaac atcagcgcgt tatcaccacc gccgagcatg acatttttct gctgttcaac
    10921 gggccagctg ttatccacaa aagacagttt tgtgctaatg ctgtcctgca cttgcccatt
    10981 ttgttgcagt tctgcgctta acaagccaga ttgctgaatg ttctgcgcca gcgaaatata
    11041 attcgcccct ttgtgcagcg tcagcgtctg gcgcatctca gcgcctgcaa atttagtcac
    11101 cagcgccacc ggttcgttat cctgctgact gaagataaac agtcctgccg acggtttgtc
    11161 gcccacgcga tacaccgttg gcatactcca cttcatgtag agattttttt ccgaacgcag
    11221 ataagcacgc ccctgcccga ccagcccgtc gccgttcatc ccacgcgcgg tgatacgcca
    11281 gcgggttaac gaatcaggca tcaggaacgt gaaatacgct ttgccttgtt tatcggttgt
    11341 gagtgacggc atccatgccg cggtatccac ctcttcacgc cgtggacgtt caagcatttt
    11401 tactcgccgc tcgctgcggt tagttgcgcc aggcgcaacc ggctcgctgg agagtgcctg
    11461 gtcgtagctg ataaacgaca gactggagct ggtacgcaca ttgttacgcc ccagcggata
    11521 gaaaaatttg ccgatattcg gcgcgatttc tggttgcagc gcgtagatca tttcatcgac
    11581 cacgcctacc gttagctgcg cagaaacagg tttacctttc agcgacgagg ttaattcgac
    11641 attgaccagt tcaccaggct ggtaatgggt tttgtccgtt ttcacccgga tatccagctg
    11701 gggaacggca actttgatcc cggcgttctg aaaactgtac tgaccgttac gggtatacag
    11761 caccgaaaaa gtgatgttag gcgcaaagga attgctcact ggaacacggg cttcatattg
    11821 agtatcgttt aaacgttgta acgtcagcca gtttgccgga tgcgatagca gcgactgctg
    11881 ttcaacgcga tcgcgttcca gcgtcaataa tgcttcatca attggctccg ggaaggtaat
    11941 cagcatcttc gcggtttcgc caggctggta cagcgtttta tctgccacga tatctaccgt
    12001 accagtatgc gacatactgc ccttaccgct gacggcatgg cttaacccgg cgagaattaa
    12061 gccgtcttta tcgcgtaacg tcagattgta gttgccaggt ttatcgaaat tgacggtaaa
    12121 ggatttgccg cctgacggta gatctccgct atggctcgtg cggtcttcga gacgcaacca
    12181 ttcatacgta acaggaacct gttttgaaga ttccagcgcg gcataacgga acacaaccga
    12241 ctcgccgcta ttactgtatt gtgcggcggt acttaaagag taatgcgcca ggccgcgttc
    12301 aatgaggatc tctttggtgg tggtgacgcg atacgccgcg ccgtcactgg cggagacggt
    12361 taacaaatag cggctcggtt tatcggcggc ggggagattt aacgccacat gaccgctggc
    12421 gtcggacacc gtttcgctgc cttccagcga cacggggaaa cgtccggcat aacgcaaatc
    12481 gttaccgacc attgataatt gctgagcgcg caaacttaac tgcacgcggg cattttttac
    12541 cggctcgcca tccgggtaga gaagttgcag tttgccgctg accgcttcgc cagttttgaa
    12601 ctcttttttg tcgagagcta aaccaatctc gaaatgtggc ttgatgtagt ttgccacgcg
    12661 aaaactgctg ctatagacct gattgcggta agcaagacgt aactcataac ctccggcaac
    12721 ggcattttct ggcaggcgga aacttccctg cccgccattg cgcgcatcca gcgtgacatc
    12781 gacggtttgc aacagactgc cgttagcgtc cagcaccgaa agcttcgccg gggcgctgac
    12841 gatgggggat gaatgcaacg gatcgtggaa ctcgcggccc atcactttaa catcgacacg
    12901 atcgcctgcg cgatatagcg ggcgatcggt aaaaatatac aagcgggtgt tgtagatttc
    12961 gctttcgtag aagaagttct cggagacaaa aacgccgcct tcagcatcct tacccagaat
    13021 gtatgaacgt tctggcgata tatgttgtaa ctgcaaggta ccgctgtcat cggtcacacc
    13081 gcgggtcatc acgccaagac cgtcagtcca caagatctct gagccgggct tcgcttcacc
    13141 ctgtttttta cccgcggtcc acaccagaag ctctttgcct gacactttgc taagcgccac
    13201 ggtatcggaa acaaacacca ccgtcgtcgc ccgataccca ccaaccatcg cctcgacgag
    13261 gtacagtccc ggctcttgtt ggccgagagg aatataaatg ttacccggct gcggcgagat
    13321 gaaattgctg gatgcgcctt ccagttttac cccttgctgc ggctcgaccg gtttagcctg
    13381 ccatagtgga taacggaact gttccaccag gggatatttt ttcagcgggg aaaactggtt
    13441 gttctgtaca taacgggaag gtttaataat ggcattgccg agctgtaatt cgggtaatgc
    13501 ctgagtcaca ttctgccgtg actgagaaga gaaagtacgc tgcatcacgc ggcgagattt
    13561 gccgtaccag ttatcccaca gccaggtcag cgtattgttc agcccgtcgc ccagatattg
    13621 cggttgcacc acaatgcgat gcaggttttt ctgctggcgc aaaaatgcca tcgggtcagg
    13681 aatacgatac aggcgaacgt ccacgccgcc gtactcttcc atctgatagc gccgataatc
    13741 acgccccggc gcttccagtc gcactttcga ctcttcactg ctgctaaaac tgctgtcagc
    13801 aagcaaaaag aatgttcccc cggcgggcgg cgcatagtta ctggaaggaa gcgaatcatc
    13861 agcattagca agccctgttc ccacaagaga cagggacaag cacgctgcta tcgcgccaga
    13921 aaatttaaac gataaatgcc aatgaaattg ggattggaat cgttgggtat ccatcgggtg
    13981 tccttccatg tcataagttg ttgcagactg actgcgcgca ttccgttgtc agttttcgtg
    14041 gcgcttccgg tgtggtagat gacgtaacgc cccatccaga ccattaagtg ctgggcatcg
    14101 ccctgatcga aaaaaatcat atcgccaggc agcgcctggt ttatgtcctg gccaataaac
    14161 tggctgttgt actgaatcaa attaatcgcg gtcacgtagg ggccggtttt cccgttcccc
    14221 tgattccagt tttgcgccag ttgacgctgt tcaggtgtta gcgtcatctc tggcggcaaa
    14281 tactggctag ataaaccgtt actttttagc catttactgt cgtgaacttt cagcgtctcg
    14341 ttcgccgcaa atcgcaccag gcccgcacaa tcctgctgat accagcgtgg acttggcccc
    14401 tggcggagct gttcttgtgc aatgcgtaca aaccaggcgc gaaacagccc ggattgttcg
    14461 acattcagca tttcgctatg ggcaacaaca caacacagcc agcaaatcag cgccagcagc
    14521 ccgtgcctca tagcggctgc cagattatgg gtagccactg ccaggcggca ccgggttcca
    14581 tctgggccag cttcatgaca taacgcggtt gttgagataa agcgtccagc ttcggcatta
    14641 ataaagtttg tgcggcgtta taaaaaaccg gttcgagatt cttcggcaga ctggtcaacg
    14701 tttcgttacg cagcagtttc gccacgcctt gtggattgat atagagcgga acgatgccat
    14761 cagtgggtat tacatccacc attgccgggc gggttttatt cagtgtttgc agggcgttat
    14821 taaccagcgt gtcatcgagg gagaaaagca gcgttttgtt ttgcatcgcc agcgacacgc
    14881 ggaaaaaata atccgacatt aattgatcgg gttgcgccgc ctgcgctttc ggatactgac
    14941 cgtatcggga actcacttcg cgacgccaga tttgcgcttc gccctgctga gtctggctta
    15001 ccggcaaaac accttctggc gctttgcttt cgtgcgcacc aatattttgc gtaaacagtt
    15061 tccctggcaa ttgcgcctgt tcggcagtgc catcaaactg accgacaaac agcggggttt
    15121 gcaattttga gtcttcatac cagcacagcc ccgcggcacc gtccaacgcc ccattcaact
    15181 tgtcgttttc ctggctgatg tgcgaaagca tctcttcggc aataccgtgt gaatacggca
    15241 ccgccacaca gaagctggcc ccggcaggca tactgttcca gaccggcgta aaatcgaaac
    15301 tggcatctac gctggcggat tcatcattta acgccacaaa actgtgccag ccgtcgttac
    15361 ccatttcgaa gcgtacgcca gcaaaagaag gcattaaccg ctggtagcca aaacccagca
    15421 acctggcgct ggccacgatg cgctggcgta ctggcgtttt ttcggcagca cgctcttcca
    15481 ggccaaagct ggcttgccag cgttttttgc cgctcaacaa atcacttgcg atcgccgtgg
    15541 cttcggtatc ctgctgatca tctttaaaca gcatatccgt gctggaaaac accagcatct
    15601 tgtcctgata tgtcgcgaac atcagggcgt tattgccgtt atagcgcaac tgataaacag
    15661 gaatagtttc actatttatc ttgatgctac tgatttccgt tttgcttaac tggctgtcgc
    15721 tggtagcggc aaataacaat ggctccagca gtttgcttaa accactgcgt tggatcagca
    15781 ccatgtaatg tgaaagatgg ccttgtttat cgtgccatag cgccgcctgc gcgggctgat
    15841 ctaagagtga cgaaaagagc ttatctttca gcgtgagatc gtgttcatag acaatgcgac
    15901 gaatgctgcc ttcaatgccc agacgatcgg catgattctg ataatagaaa acgaaatctt
    15961 cactcagaac atcgtggaga aacggaatgg tgaggagatc tttgggaagc tggcttagag
    16021 aatcgctgtc gagaaagagg tccggctcat tgagatcaat ttgcaaatta ttgtgcacca
    16081 ccagcggcga caacgttttt tctggcccac tgccagcata ttgcaacgcc cagacgccag
    16141 cggaaagcag tgctattgcg ccaaaaccta caagaccata gaaccgccag cctttcgctt
    16201 tttttcacca ctcattgcca cattccttgt gtatagccag ccatttttta cggacacagc
    16261 caaactttac cgcgccctaa tacgacaaaa gcccagactt tgcagcctgg acttttcaat
    16321 tcaaaaaagg gagatggctc ccttttggca tgaagaagta aaattattct tcttctggct
    16381 cgtcgtcaac gtccacttcc ggagcgattt catcgtcccc ttccgcggca ctgccgtcga
    16441 tggtatccag atcttcctcg tcaaccggtt cagcaacacg ttgcagaccc actacgtttt
    16501 catcttccgc agtacggatg aggatcacgc cctgggtgtt acggcccacg atgctgattt
    16561 ctgaaacgcg agtacgtacc agcgtaccgg catcggtgat catcatgatc tggtcgcagt
    16621 catctacctg taccgcgcca acaactaaac cgttacgttc ggtaaccttg atggagataa
    16681 cccctttcgt cgcacgcgac ttagtcgggt attccgccac tgcggtacgt ttaccataac
    16741 cgttctgcgt tgcggtgagg attgcgccat cgccacgagg cacgatcaga gagacgactt
    16801 tatcaccttc gcctaaacga ataccgcgaa caccggtggt gttgcagccc atcgcacgga
    16861 cagaagactc tttaaagcgc accactttac cttcggcgga gaacagcatt acttcgtctt
    16921 cgccgctggt caggtcaacg ccgatcagct catcgccttc aaccagtttg atcgccactt
    16981 taccggcggt acgcagacgg ttgaactcgg tgaggacggt tttcttcaca gtaccgttag
    17041 cggtcgccat gaagactttc acgccttctt caaactcggt caccggcagg atcgcggtga
    17101 tacgttcgtc ctgctccagc ggcagcaggt tgacgatcgg acgaccgcgc gcgccacgag
    17161 tggcctccgg caactggtaa actttcatcg aatagacgcg accacggctg gagaagcaca
    17221 gaatatggtc gtgcgtgttc gccaccagca gtcggtcgat aaagtcttct tctttaatac
    17281 gtgcggcaga tttacctttc ccgccacgac gctgcgcttc gtattcagaa agcggctgat
    17341 atttaacgta gccctggtga gagagcgtca cgaccacatc ttcctgggtg atcagatctt
    17401 ccaggttgat gtctgcgctg ttggcggtga tttcagtacg acgtttgtca ccgaactgtt
    17461 cacgaaccag ctccagctct tcacggatca cttccatcag acgatcggcg ctaccaagaa
    17521 tacgcagcag ttccgcgatc tgatccagca gctctttgta ttcgtcgagc agtttttcgt
    17581 gctcaaggcc ggtcagtttc tgcaaacgca gatccagaat tgcctgagct tgctgttcgg
    17641 tcaggtagta cagaccatca cgcacgccga actctggctc cagccattcc ggacgcgcag
    17701 catcgtcgcc agcacgttcg agcatcgcgg caacgttgcc cagctgccac ggattagcca
    17761 ccagcgcagt tttcgcttct gcaggcgtcg gcgcatgacg gatcagttcg atgatcgggt
    17821 cgatgttcgc cagcgccacg gctaatgctt caaggatatg agcacgatcg cgagctttac
    17881 gcagttcgaa aatagtacga cgggtcacca cttcacggcg gtgacgaaca aacgccgcga
    17941 tgatgtcttt caggttcatg atctttggct gaccatggtg caatgccacc atgttgatac
    18001 cgaaagaaac ctgcaactgg gtctgggagt agaggttgtt gagcacaact tcaccgaccg
    18061 catcgcgttt cacttcaatc acgatgcgca taccgtcttt gtcagactcg tcacgcagcg
    18121 cgctgatgcc ttccacgcgt ttttctttta ccagttccgc aatcttctcg atcaggcgcg
    18181 ctttgtttac ctgatacgga atttcgtgga cgataatggt ttcacgtccg gttttggcgt
    18241 caacttccac ttcagcacgg gcgcggatat ataccttgcc gcgaccggta cggtaagctt
    18301 cttcaatacc gcgacgaccg ttaatgattg ccgccgtcgg gaagtccggc cccgggatgt
    18361 gttccatcag cccttcaatg ctgatgtctt catcatcgat atacgccaga caaccgttga
    18421 tgacttccgt caggttgtgc ggcgggatgt tggttgccat acctacggcg ataccggaag
    18481 aaccgttcac cagcaggtta ggaattttgg ttggcatgac gtcgggaatt ttttccgtgc
    18541 cgtcatagtt atcaacgaaa tcgaccgtct ctttttcgag atcggccatc agttcatggg
    18601 caattttcgc cagacggatt tccgtataac gcattgccgc cgcagagtcg ccgtcgatgg
    18661 aaccgaagtt accctgaccg tctaccagca tgtaacgcag cgagaatggc tgcgccatac
    18721 ggacgatcgt gtcataaact gccgagtcac catggggatg gtatttaccg attacgtcac
    18781 caacgacacg ggcagatttt ttataggctt tgttccagtc attgcctagt acgttcatgg
    18841 cgtaaagtac gcgacggtgt accggcttca ggccatctcg gacatctggc agcgcacggc
    18901 caacaatgac cgacatcgca taatccagat aggagctctt cagctcttcc tcaatgttga
    18961 ccggtgtaat ttctctcgca aggtcgctca tctaaccgct atccctctac tgtatcccgg
    19021 attcaaaggt cgcaaattat aacacagccg cgcagattga ggtaaaccta tacgctttat
    19081 tcacatccaa tgcctgatat actcgtttgt cttgccaatt acggagtaga agtgccaatg
    19141 aatgccgaaa aatcgccggt aaaccataac gtagaccacg aagagatcgc taaatttgaa
    19201 gccgtcgcct cccgctggtg ggatctggaa ggtgagttca aaccgctgca ccgcattaac
    19261 ccgctgcgtc tgagctatat tgccgagcgt gctggcggct tatttggcaa aaaggtgctc
    19321 gatgtcggtt gtggcggcgg cattctggcc gagagtatgg cgcgcgaagg cgcgacggtg
    19381 accggtctgg atatgggctt tgagccattg caggtggcaa agctgcacgc gctggaaagc
    19441 ggcattcagg tggattacgt gcaggaaacc gtggaagagc acgcggcaaa acatgccggg
    19501 cagtatgatg tggtgacctg catggagatg ctggagcacg tccctgatcc gcagtcagtg
    19561 gtcagagcct gtgcgcaact ggtgaaaccg ggcggcgatg tctttttctc gacgcttaac
    19621 cgcaacggca agtcatggct gatggcggta gtcggcgcgg aatatatttt gcgcatggtg
    19681 cctaaaggta cacatgatgt gaagaagttt attaaaccgg cagaactgct gggctgggta
    19741 gatcagacaa gccttaaaga gcgacatata accgggctac attacaaccc gatcactaat
    19801 acttttaaac tcggccccgg cgtggatgtg aactatatgc tgcacacgca gaataagtga
    19861 ggttgttgtt tggattcatc cggcaaccgc gccaatgcct gatgcgacgc ttgccgcgtc
    19921 ttatcaggcc tacaaatgct ctccgtaggc ctgattaggc gtttacgccg catccagcaa
    19981 tagtgatggt cagacagcga ttaccatttc accgtcatcg acaaaaaccc tgccgtctgg
    20041 gcaaaatcat cactcccttt ctgccacgcc acgctaccac gcagcgaaac tcgctgactg
    20101 atattgcccg tgactcccac ttttatttca ccccgttgct tcaccgcatc gtcactgata
    20161 gtgctggcat cttcttcaat ttccgtcgaa tggggatcgt gataataatt cagatctaat
    20221 gttggtatga catgaacagc ggtacgccat tcgctgtgta aacccagccg cgtctgaata
    20281 tcatcaccct gcgattgtga cacgcgcgca cggttagcgg cggtaaaatc atcctgctgc
    20341 acgccctgat aaatcacctg cgcctgcggt tcaatcacca caccacgccc cggtaaccac
    20401 tgataccccg cttccagcga ggcgataatc cccgacgaat ggtaatgatc cacgccatct
    20461 tcatgttcag aaacatcatt gctaaaccac gcgtactgca accagttatc cagccaggcc
    20521 ccctgcttct gcttaccgtg ctgaaaccag cttgaggtca gcccaaccgc ataaccgtgg
    20581 ttctggttat cggcgcgagt tccggtcata ctcgagcggc tgtcgccctg gttatcgctg
    20641 tagccaccaa caatcccaag catccactcg ccatccgtgc cccagcgccc gctaaacaga
    20701 tcgccgctaa gttgcaccgt agaagtgtct tcatgttgag ccagttgccc cgctgctgtg
    20761 taatgataat ctccgccgat aacacgtaaa ttcagcgtct gaccatcgcc acctgcgtga
    20821 tcgcgtcgct ccatcataaa cgcctgattt gccgcccgca ggttattgag ataaccgcca
    20881 actttggcat tcaacaccgg ctggtaagca ggcgtaggtt ccgggtcggg tgttggatca
    20941 ggatcctgcg tgggatcagg atcgggagtc gggtctggat caggtggcga tggcggcgtt
    21001 acttcttgcg atcgcagata ccagtcgttg ttatcttcca ccagcgtgta gtcatacgct
    21061 cccatattga cgtagccgct gcctgccaga ctgaactgcg cattgttttg aaactgcgtg
    21121 ggatcagctg cgaaatcaac cactttaatg cctgtcgatg tcggctcacc aatccctgta
    21181 atggagttaa ccaccacagt tgtgttgcca gcagtattac cgttcatcac caattgatcg
    21241 cttaccgagt catcgccgtt taattcgcta tcgagcagta gcgtaccgcc gccggtataa
    21301 tcaccgttta ccgtcagcgt atcgccagcg acgccatttt gcaggctaac cgcaccggac
    21361 gtattggtca gattgccatt aaccgtcacg ttggaaagat aagtcaggct gtcagtttca
    21421 aggttggtat taagctcacc tgtcaacgtc acaccatcgg tggcattcac cagcgtccca
    21481 tcgcagttaa acagtgaggc attaagggcg acggaagtat cctgcccggt caggcgcaaa
    21541 accgtaccat cgctgatgtc gatagtgccg ctggaagtag catcaattga ctgaatatcc
    21601 tgatccgcgc cagtaacgaa cgttgccccg tcaccgaccc atagcgacga agcataaggg
    21661 aagatatccg caacatcacc ttgcagcgtg ccctcttcta ctctcaccgc tgactgcgtt
    21721 gtaccgctgg cggtcagctc cagtgtaccc gcccccgttt tagtcaatgt gctaccctca
    21781 tcctgatgct gcccgctgct gtcagccatc agtgcgcccc actgcgtgtc tacccccgca
    21841 tcaactgcca cttcaccgtc ggcgcgcatt tcaatatcac gcccatgctc agtcgttgta
    21901 gagcgagtat cgacattggc agtaatctcc atcacgcttt cttgtggctt atcggtaaag
    21961 ataacctggc ggttataatg ggtatatcca agctgcgagt tgtcgctcac catcagcgta
    22021 ccggaagcga tttgcgttgt gccgaggtat tgattgtcat ttgccaggct gacctgcccg
    22081 tcacccgcat cgatcaccac gtagacgcca tctttcccgt cgccgccgat atgcatatcg
    22141 tttccgcctg caagatctac cacatcctga ctgccgataa ccgttccgct gctgccactg
    22201 acttcaaggc catctttgta tgatgtaccg ctctgccagg tggagaaatc agagagatcg
    22261 agcataccgc cgtttaacac gatcgactgc ggatcgtcct ggagagcggc aagatctgcc
    22321 gcgtcgcctt ccagcgaaag caccgcacca gcatccacca ctatatcgcc ggttagcgcc
    22381 atcgactgcg cccccgccag cacatagctg ccgttttgcg caatggtgag ctgaccagca
    22441 ccttcgatgg tgccagcaaa actgccttga ttaacagtaa cattgccacc agcatcgata
    22501 tttaaagtgc cattctgaaa gcccgtcaat gagtgcgcaa aggtttgttg ggtggagcca
    22561 acatttagct ctgcctgatt ctggtactta tcaatactcc ctatcgtcag accgtagcag
    22621 tcttgcggat cgtcctggca atgcgtatcg ccgacattca tcagggagtt gctgcggccc
    22681 agggtaactt caccgttttc aatctgcatc tcaccagtaa agtcattgtt atctgcatta
    22741 agtaccagat cgccggaacc tgttttggtg attaacccgg taccagcaat agagtcaaca
    22801 gctccgtcat tctctgtatt gccaataacc agcgtttttc cgtcggcaat atcaaaggta
    22861 acttcactta agccgagata cataaagcca cccgccgcag aggaaggacc atctccatag
    22921 cctgctgcgc tattgttctc atcgactaac acgcctccgt tctggctgta gctgtcatca
    22981 acagaaatat caataagata gggagccgtc gcgctattgg tatatatcgc cccgccataa
    23041 ccttcggcag tgttatttgt aaaggcagtg ttatttatta tcgtataacc tgaaggatgc
    23101 ttgctgtcgc tattattatc tgtgacatcg attgcgccgc catcgccatc actgtaactt
    23161 gttgatgtat atgcctggtt attgttaaaa acatcatcac ttaaatagat atcattattg
    23221 atggtataaa ttgcaccacc tttgccgtca ttagcgatgt tattgcgaaa cacggcgtta
    23281 gtgacacgta aatcgatggc accggtatcg ttagtaccag aagaatagat tgcgccacca
    23341 tagccgcctg cgacgttacc agaaaaaata acatccgtaa gattcagcgt tgagttttct
    23401 ttagcaaata ttgccccgcc gttattatat tctcctgaaa cgatgttatt ggcaaacaga
    23461 gtcattccag tttcattttc tggtgataat gtaaattccg ctccttgttg caaaaatacg
    23521 gccccaccgc tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcggcaata
    23581 ctccagacct gcgtaatgcc tgaaaggctt tgcctgctgg cgtgacaact cgctttgata
    23641 ttatatccct ggcataaatc aatggccgcc gcgacaccgt tagcagggaa aagagatgca
    23701 atcattgacg ggagtagaga taaatactcc ttgcgtagaa agataacccg catattaatc
    23761 tgaccatcat tttatataag acggcatatt taaacgcatt atacaatatg ggttatcttt
    23821 cttttgtaaa ttgtccaacc cgatgcaata gc
//
LOCUS       DBFIFS010000049        23845 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.049, whole genome shotgun sequence.
ACCESSION   DBFIFS010000049 DBFIFS010000000
VERSION     DBFIFS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.049"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(76..1341)
                     /locus_tag="N7A07_001835"
     CDS             complement(76..1341)
                     /locus_tag="N7A07_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2476511.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="N7A07_001836"
     tRNA            complement(1539..1614)
                     /locus_tag="N7A07_001836"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="N7A07_001837"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="N7A07_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU2476512.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="N7A07_001838"
     CDS             2580..2663
                     /locus_tag="N7A07_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476513.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="N7A07_001839"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="N7A07_001839"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU2476514.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="N7A07_001840"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="N7A07_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU2476515.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="N7A07_001841"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="N7A07_001841"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU2476516.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="N7A07_001842"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="N7A07_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU2476517.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="N7A07_001843"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="N7A07_001843"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU2476518.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="N7A07_001844"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="N7A07_001844"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU2476519.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="N7A07_001845"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="N7A07_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU2476520.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="N7A07_001846"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="N7A07_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU2476521.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="N7A07_001847"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="N7A07_001847"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU2476522.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="N7A07_001848"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="N7A07_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU2476523.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     IQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="N7A07_001849"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="N7A07_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU2476524.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="N7A07_001850"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="N7A07_001850"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HGU2476525.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSACYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="N7A07_001851"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="N7A07_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU2476526.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="N7A07_001852"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="N7A07_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU2476527.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="N7A07_001853"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="N7A07_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU2476528.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="N7A07_001854"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="N7A07_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU2476529.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="N7A07_001855"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="N7A07_001855"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU2476530.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="N7A07_001856"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="N7A07_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU2476531.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="N7A07_001857"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="N7A07_001857"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU2476532.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="N7A07_001858"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="N7A07_001858"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU2476533.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="N7A07_001859"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="N7A07_001859"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU2476534.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="N7A07_001860"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="N7A07_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU2476535.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="N7A07_001861"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="N7A07_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU2476536.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5864 a         6003 c         6253 g         5725 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatatccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacat gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacctg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgac
//
LOCUS       DBFIFS010000050        23711 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.050, whole genome shotgun sequence.
ACCESSION   DBFIFS010000050 DBFIFS010000000
VERSION     DBFIFS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.050"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            77..1741
                     /gene="mhpA"
                     /locus_tag="N7A07_001862"
     CDS             77..1741
                     /gene="mhpA"
                     /locus_tag="N7A07_001862"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU2476537.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            1743..2687
                     /gene="mhpB"
                     /locus_tag="N7A07_001863"
     CDS             1743..2687
                     /gene="mhpB"
                     /locus_tag="N7A07_001863"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU2476538.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            2705..3571
                     /gene="mhpC"
                     /locus_tag="N7A07_001864"
     CDS             2705..3571
                     /gene="mhpC"
                     /locus_tag="N7A07_001864"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU2476539.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            3581..4390
                     /gene="mhpD"
                     /locus_tag="N7A07_001865"
     CDS             3581..4390
                     /gene="mhpD"
                     /locus_tag="N7A07_001865"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU2476540.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            4387..5337
                     /gene="mhpF"
                     /locus_tag="N7A07_001866"
     CDS             4387..5337
                     /gene="mhpF"
                     /locus_tag="N7A07_001866"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU2476541.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            5334..6347
                     /gene="mhpE"
                     /locus_tag="N7A07_001867"
     CDS             5334..6347
                     /gene="mhpE"
                     /locus_tag="N7A07_001867"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU2476542.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHRYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            6523..7734
                     /gene="mhpT"
                     /locus_tag="N7A07_001868"
     CDS             6523..7734
                     /gene="mhpT"
                     /locus_tag="N7A07_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU2476543.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            7836..8375
                     /gene="yaiL"
                     /locus_tag="N7A07_001869"
     CDS             7836..8375
                     /gene="yaiL"
                     /locus_tag="N7A07_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU2476544.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(8500..9333)
                     /gene="fghA"
                     /locus_tag="N7A07_001870"
     CDS             complement(8500..9333)
                     /gene="fghA"
                     /locus_tag="N7A07_001870"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU2476545.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(9426..10535)
                     /gene="frmA"
                     /locus_tag="N7A07_001871"
     CDS             complement(9426..10535)
                     /gene="frmA"
                     /locus_tag="N7A07_001871"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU2476546.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(10570..10845)
                     /gene="frmR"
                     /locus_tag="N7A07_001872"
     CDS             complement(10570..10845)
                     /gene="frmR"
                     /locus_tag="N7A07_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU2476547.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(11005..12051)
                     /gene="fbpC"
                     /locus_tag="N7A07_001873"
     CDS             complement(11005..12051)
                     /gene="fbpC"
                     /locus_tag="N7A07_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HGU2476548.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(12063..14141)
                     /locus_tag="N7A07_001874"
     CDS             complement(12063..14141)
                     /locus_tag="N7A07_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2476549.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(14210..15241)
                     /locus_tag="N7A07_001875"
     CDS             complement(14210..15241)
                     /locus_tag="N7A07_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2476550.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(15238..16554)
                     /gene="uhpC"
                     /locus_tag="N7A07_001876"
     CDS             complement(15238..16554)
                     /gene="uhpC"
                     /locus_tag="N7A07_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU2476551.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(16627..18168)
                     /locus_tag="N7A07_001877"
     CDS             complement(16627..18168)
                     /locus_tag="N7A07_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU2476552.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(18168..18797)
                     /locus_tag="N7A07_001878"
     CDS             complement(18168..18797)
                     /locus_tag="N7A07_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGU2476553.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPIFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            19100..20062
                     /gene="tauA"
                     /locus_tag="N7A07_001879"
     CDS             19100..20062
                     /gene="tauA"
                     /locus_tag="N7A07_001879"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2476554.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            20075..20842
                     /gene="tauB"
                     /locus_tag="N7A07_001880"
     CDS             20075..20842
                     /gene="tauB"
                     /locus_tag="N7A07_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU2476555.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     IQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            20839..21666
                     /gene="tauC"
                     /locus_tag="N7A07_001881"
     CDS             20839..21666
                     /gene="tauC"
                     /locus_tag="N7A07_001881"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU2476556.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            21663..22514
                     /gene="tauD"
                     /locus_tag="N7A07_001882"
     CDS             21663..22514
                     /gene="tauD"
                     /locus_tag="N7A07_001882"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU2476557.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(22621..23595)
                     /gene="hemB"
                     /locus_tag="N7A07_001883"
     CDS             complement(22621..23595)
                     /gene="hemB"
                     /locus_tag="N7A07_001883"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU2476558.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         5481 a         6241 c         6671 g         5318 t
ORIGIN      
        1 ttacatattt ttaacaaaat aagttgcgct gtactgtgcg cgcaacggca ttttgtccga
       61 gtcgtgaggt actgaaatgg caatacaaca ccctgacatc cagcctgctg ttaaccatag
      121 cgttcaggtg gcgatcgctg gtgccggtcc ggtcgggctg atgatggcga actatctcgg
      181 ccagatgggc attgacgtgc tggtggtgga gaaactcgat aagttgatcg actacccgcg
      241 tgcgattggt attgatgacg aggcgctgcg caccatgcag tcggtcggcc tggtcgataa
      301 tgttctgccg catactacgc cgtggcacgc gatgcgtttt ctcaccccga agggccgctg
      361 ttttgctgat attcagccaa tgaccgatga atttggctgg ccgcgccgta acgcctttat
      421 tcagccgcag gtcgatgcgg tgatgctgga aggggtgtcg cgttttccga atgtgcgctg
      481 cttgttttcc cgcgagctgg aggccttcag tcagcaagat gacgaagtga ccttgcacct
      541 gaaaacggca gaagggctgc gggaaatagt caaagcccag tggctggtgg cctgtgatgg
      601 tggggcaagt tttgtccgtc gcaccctgaa tgtgccgttt gaaggtaaaa ctgcgccaaa
      661 tcagtggatt gtggtagata tcgccaacga tccgttaagt acgccgcata tctatttgtg
      721 ttgcgatccg gtgcgcccgt atgtttctgc cgcgctacct catgcggtac gtcgctttga
      781 atttatggtg atgccgggag aaaccgaaga acagctgcgt gagccgcaaa atatgcgcaa
      841 gctgttaagc aaagtgctgc ctaatccgga caatgttgaa ttgattcgcc agcgtgtcta
      901 cacccacaac gcgcgactgg cgcaacgttt ccgtattgat cgcgtactgc tggcgggcga
      961 tgccgcgcac atcatgccgg tatggcaggg gcagggctat aacagtggta tgcgcgacgc
     1021 ctttaacctc gcatggaaac tggcgttggt tatccagggg aaagcccgcg atgcgctgct
     1081 cgatacctat caacaagaac gtcgcgatca cgccaaagcg atgattgacc tgtccgtgac
     1141 ggcgggcaac gtgctggctc cgccgaaacg ctggcagggt acgttacgtg acggcgtttc
     1201 ctggctgctg aattatctgc cgccagtaaa acgctacttc ctcgaaatgc gcttcaagcc
     1261 gatgccgcaa tattacggcg gtgcgctggt gcgtgagggc gaagcgaagc actctccggt
     1321 cggcaagatg tttattcagc cgaaagtcac gctggaaaac ggcgacgtga cgctgctcga
     1381 taacgcgatc ggcgcgaact tcgcggtaat tggctgggga tgcaatccac tgtgggggat
     1441 gagcgacgag caaatccagc agtggcgcgc gttgagcaca cgcttcattc aggtggtgcc
     1501 ggaagtgcaa attcataccg cacaggataa ccacgacggc gtactacgcg tgggggatac
     1561 gcaaggtcgc ctgcgtagct ggttcgcgca acacaatgct tcgctggtgg tgatgcgccc
     1621 ggatcgcttt gttgccgcca ccgccattcc gcaaacactg ggcaagaccc tgaataaact
     1681 ggcgtcggtg atgacgctga cccgccctga tgccgacgtt tctgtcgaaa aggtagcctg
     1741 atatgcacgc ttatcttcat tgtctttccc actcgccgct ggtggggtat gtcgacccgg
     1801 cacaagaggt actcgatgag gtcaatggcg tgattgccag cgcccgcgag cgtattgcgg
     1861 ctttctcccc tgaactggtg gtgctgtttg cgccagatca ctacaacggc tttttctatg
     1921 acgtgatgcc accgttctgt ttaggcgttg gagcgacggc aattggtgat ttcggcagtg
     1981 cggcaggaga gctgcccgtg cctgtggagc tggcggaggc ctgtgcgcat gccgtcatga
     2041 agagcgggat cgatcttgcc gtttcttact gtatgcaggt ggaccacggg ttcgcccagc
     2101 cgctggagtt cctgctcggt gggctggata aggtgccagt tctgcctgtg ttcatcaacg
     2161 gtgtcgccac gccgctgccc ggtttccagc gtacccgcat gttgggtgaa gccattggac
     2221 gtttcaccag cactctcaat aaacgcgtgc tgttcctagg ttccggtggg ctttcccatc
     2281 agccgccggt gcccgaactg gcgaaagccg atgcccatat gcgcgaccgc ctgttgggga
     2341 gcgggaaaga tttacccgcc agtgagcgcg aattgcgtca gcaacgggtg attagcgccg
     2401 ctgagaagtt tgttgaggat cagagaactc tgcatccgct caacccgatt tgggataacc
     2461 agttcatgac tttgctggag cagggacgca tacaggaact ggatgccgtc agtaacgaag
     2521 agctttccgc cattgccgga aagtcgacac atgaaatcaa aacctgggtc gccgcttttg
     2581 ccgctatttc tgcatttggc aactggcgta gcgaagggcg ttattaccgc ccaatcccgg
     2641 agtggattgc cggatttggc tcgttaagcg ccagaacaga gaactgaata tgcaggagaa
     2701 gatgatgagt tatcagccac aaaccgaagc cgccaccagc cgttttctga atgtagaaga
     2761 agcgggtaaa acgctgcgca tccattttaa tgactgcgga caaggcgacg aaaccgttgt
     2821 cctgctgcat ggttccggcc cgggtgctac tggctgggcg aacttcagcc gcaatatcga
     2881 tccgctggta gaggcgggct atcgggtgat cctgctggat tgtccgggtt ggggcaagag
     2941 cgattcgatc gttaatagtg gttcgcgatc ggatcttaat gcacgaatcc tgaaaagcgt
     3001 ggtggatcaa ctggatatcg ccaaaatcca cctgctgggc aactcgatgg gtggccatag
     3061 ttctgtggcg ttcaccctta actggccgga gcgcgtcggc aaactggtgc tgatgggcgg
     3121 cggtacgggc ggcatgagtt tgtttacgcc gatgccaacc gaaggtatta agcgactgaa
     3181 tcagctttat cgtcagccga ctatcgaaaa cctgaagctg atgatggata tcttcgtttt
     3241 tgataccagc gatttgaccg acgccctgtt tgaagcgcgc ctgaataata tgctgtcgcg
     3301 ccgcgatcac ctggaaaact tcgttaagag cctggaagct aatccgaaac agttcccgga
     3361 ttttggccca cgtctggcgg aaatcaaagc gcaaaccctg attgtctggg ggcgcaacga
     3421 ccgctttgtg ccgatggatg cgggtctgcg tctgctgtcc ggcattgccg gttctgaact
     3481 gcatatcttc cgcgactgtg ggcattgggc gcagtgggaa catgccgacg ctttcaatca
     3541 actggtgctg aatttcctcg cacgtgctta aggaacggtc atgacgaagc atactcttga
     3601 gcaactggcg gcggatttac gccgcgccgc agagcagggc gaagcgattg cgccgttgcg
     3661 cgatctgatt ggtatcgata acgctgaagc ggcctacgcc attcagcaca taaatgtgca
     3721 atatgacgtt gcgcaggggc gtcgcgtggt ggggcgtaaa gtgggcctga cacatccgaa
     3781 agtgcagcaa caactgggcg ttgatcaacc ggatttcggg acattatttg ccgacatgtg
     3841 ttatggcgat aacgaaatca ttcctttttc ccgtgttctg caaccccgca ttgaagcgga
     3901 gatcgcactg gtgttgaacc gcgatttacc cgcaactgat atcaccttcg acgaattgta
     3961 taacgccatt gaatgggtac ttccggcgct ggaggtggtg ggtagccgca ttcgcgactg
     4021 gtcgattcag tttgtcgata ccgtggcaga taacgcttcc tgcggggtgt atgtcatcgg
     4081 cggtccggcg caacgtccgg cggggttaga cctgaaaaac tgcgccatga agatgacgcg
     4141 taataacgaa gaggtttcca gcgggcgcgg cagcgaatgc ctgggacatc cgcttaatgc
     4201 cgccgtctgg ctggcacgca aaatggccag tctgggtgaa ccgctgcgcg ccggagatat
     4261 cattcttacc ggggcattag gtccgatggt ggcagtgaat gcgggcgatc gttttgaagc
     4321 ccatattgaa ggcataggtt cagttgctgc ggcattttca agcgcagccc caaaaggaag
     4381 tctgtcatga gtaagcgtaa agtcgccatt atcggttctg gcaacattgg caccgatctg
     4441 atgattaaaa ttttgcgtca cgatcagcat ctggagatgg cggtgatggt cggcattgat
     4501 cctcagtccg acggtctggc gcgtgccaga cgtatgggcg tcgccaccac ccatgaaggg
     4561 gtgatcgggc tgatgaacat gcctgaattt gctgatatcg acattgtatt tgatgcgacc
     4621 agcgccggtg ctcatgtgaa aaacgatgcg gctttgcgcg aagcgaaacc ggatattcgc
     4681 ttaattgacc tgacgcctgc tgccatcggc ccttactgcg tgccggtggt taacctcgag
     4741 gagaacgtcg atcaactgaa cgtcaacatg gtcacctgcg gcggccaggc caccattcca
     4801 atggtggcgg cagtttcacg cgtggtgcgt gttcattacg ccgaaattat cgcttctatc
     4861 gccagtaaat ctgccggacc tggcacgcgt gccaatatcg atgaatttac ggaaaccact
     4921 tcgcgagcca ttgaagtggt gggcggcgcg gcaaaaggga aggcgattat cgtgcttaac
     4981 ccagcagagc caccgttgat gatgcgtgac acggtgtatg tattgagcga cgaagcttca
     5041 caagatgata tcgaagcctc aatcaatgaa atggctgagg cggtgcaggc ttacgtaccg
     5101 ggatatcgcc tgaaacagcg cgtgcagttt gaagttatcc cgcaggataa accggttaat
     5161 ttgccgggcg tagggcagtt ctccgggctg aaaacggcgg tctggctgga agtcgaaggc
     5221 gcagcgcatt atctgcctgc ctatgcgggc aacctcgaca ttatgacttc cagtgcgctg
     5281 gcgacagcag aaaaaatggc ccagtcactg gcgcgcaagg caggagaagc ggcatgaacg
     5341 gtaaaaaact ttatatctcg gacgtcacgt tgcgtgacgg tatgcacgcc attcgtcatc
     5401 ggtattcgct ggaaaacgtt cgccagattg ccaaagcact ggacgatgcc cgcgtggatt
     5461 cgattgaagt ggcccacggc gacggtttgc aggggtccag ctttaactat ggtttcggcg
     5521 cacatagcga ccttgaatgg attgaagcag cggcggatgt ggtgaagcac gccaaaatcg
     5581 cgacgttgtt gctgccagga atcggcacta ttcacgatct gaaaaatgcc tggcaggctg
     5641 gcgcgcgggt ggttcgtgtg gctacgcact gtaccgaagc tgatgtttcc gcccagcata
     5701 ttcagtatgc ccgcgagctc ggaatggaca ccgttggttt tctgatgatg agccatatga
     5761 ccacgccgga gaatctcgca aagcaggcaa agctgatgga aggctacggt gcgacctgta
     5821 tttatgtggt ggattctggc ggtgcgatga acatgagcga tatccgtgac cgtttccgcg
     5881 ccctgaaagc agtgctgaaa ccagaaacgc aaaccggcat gcacgctcac cataacctga
     5941 gtcttggcgt ggcgaactct atcgcggcgg tggaagaggg ctgcgaccga atcgacgcca
     6001 gcctcgcggg aatgggagcg ggcgcaggta acgcgccgct ggaagtattt attgccgccg
     6061 tggataaact gggttggcag catggggccg atctctatgc gttaatggat gctgctgacg
     6121 acctggtgcg tccgttgcag gatcgaccgg tacgagtcga tcgcgaaacg ctggcgctgg
     6181 gatacgctgg tgtttactcc agcttcctgc gtcactgtga aacggcggcg gcgcgttatg
     6241 gcttaagcgc ggtggatatt ctcgttgagc tgggcaaacg ccggatggtt ggcggccagg
     6301 aggatatgat cgtcgacgtg gcgctggatc tgcgcaacaa caaataatat tgactgccga
     6361 gaacgtgcat tttgtatgcc catcccagca cgactgccgg atgcggcgtg aacgccttat
     6421 ccggtctaca tttcgcccgt aggcagtcat taaatagttc tgattacgcg gctggcaatg
     6481 tatcagtcgc gattcacctg cacacgcaat gaggttcatc ttatgtcgac tcgtacccct
     6541 tcatcatctt catcccgcct gatgctgacc atcgggcttt gttttttggt cgctctgatg
     6601 gaagggctgg atcttcaggc ggctggcatt gcggcgggtg gcatcgccca ggctttcgca
     6661 ctcgataaaa tgcaaatggg ctggatattc agcgccggaa tactcggttt actacccggc
     6721 gcgttggttg gcggaatgct ggcggaccgt tatggtcgca agcgcatttt gattggctca
     6781 gttgcgctgt ttggtttgtt ctcgctggca acggcgattg cctgggattt cccctcactg
     6841 gtctttgcgc ggctgatgac cggtgtcggg ctgggggcgg cgttgccgaa tcttatcgcc
     6901 ctgacgtctg aagccgcggg tccacgtttt cgtgggacgg cagtgagcct gatgtattgc
     6961 ggtgttccca ttggcgcggc gctggcggcg acactgggtt tcgcgggggc aaacttagca
     7021 tggcaaacgg tgttttgggt aggtggtgtg gtgccgttga ttctggtgcc gctattaatg
     7081 cgctggctgc cggagtcggc ggttttcgct ggcgaaaaac aggctgcgcc accactgcgt
     7141 gcgttatttg cgccagaaac ggcaaccgcg acgctgctgc tgtggctgtg ttatttcttc
     7201 actctgctgg tggtttacat gttgatcaac tggctaccgc tgcttttggt ggagcaagga
     7261 ttccagccat cgcaggcggc aggggtgatg tttgcactgc aaatgggtgc ggcgagcggg
     7321 acgttaatgt tgggcgcatt gatggataag ctgcgtccag taaccatgtc gctactgatt
     7381 tatagcggca tgttagcttc gctgctggcg cttggaacgg tgtcgtcatt taacggtatg
     7441 ttgctggcgg gatttgtcgc ggggttgttt gcgacaggtg ggcaaagcgt tttgtatgcc
     7501 ctggcaccgt tgttttacag ttcgcagatc cgcgcaacag gtgtgggaac agccgtggcg
     7561 gtagggcgtc tgggggctat gagcggtccg ttactggccg ggaaaatgct ggcattaggc
     7621 actggcacgg tcggcgtaat ggccgcttct gcaccgggta ttcttgttgc tgggttggcg
     7681 gtgtttattt tgatgagtcg gagattaaga atgcagccgt gtgccgatgc ctgacgcgcc
     7741 ttattaagca ggggcgaaag ggacttgccc ccaatacacc cacggcgttt acaggtatac
     7801 tcgctaaaaa ttattcagcg ggtttggaaa caaagatggc aaaacttacc ttacaagagc
     7861 agttgctcaa agcaggatta gtcaccagca aaaaagcggc gaaggtcgag agaacggcga
     7921 aaaaatcgcg cgttcaggcg cgtgaagctc gggcggcggt agaagaaaat aaaaaggcac
     7981 agcttgagcg tgataaacag cttagcgaac agcaaaaaca agcggcgttg gcgaaagaat
     8041 ataaagctca ggtgaagcag cttattgaaa tgaaccggat caccattgcc aatggcgata
     8101 ttggttttaa cttcactgat ggcaatctga ttaagaagat ttttgtcgat aagctcacac
     8161 aggcgcagtt gattaatggt cgtctggcga ttgcccgctt gttggtcgat aacaatagcg
     8221 aaggtgaata cgccattatt cccgccagcg ttgccgataa aattgctcag cgtgatgcca
     8281 gcagtattgt gttgcacagc gcgctcagtg ccgaagagca ggatgaagac gacccgtatg
     8341 ccgacttcaa agtgcctgat gatttgatgt ggtaaacata gataagtcgt gcgcagatgc
     8401 ctgatgcgac gctatgcgcg tcttatcatg cctacggcct gcgggcgaaa tgtaggcgga
     8461 taaggcgttc acgccgcatc cggcagtcgt gcactattat caacgcatat tcagtttatt
     8521 ggcgtggtag gcaatatgct cgccaataaa actggagaca aaataatagc tgtggtcgta
     8581 accctcgtga taacggatta acgttttgat attcatctcc tggcagatct tttcaagatt
     8641 ttgagtccgc agctgttccg cgtaaaaatc atcactcaac ccctgatcaa ccatgatttc
     8701 cgcaacgcgt tgaccttgtg aaataagact caccggatgg taatccaacc aggcctcttt
     8761 attttcacca agatatgcgg caaaggcttg ctgtccccac ggtacttgcg atggggagac
     8821 aatgggcgaa aacgccgaga cgctgacata ttcatctggg ttacgtaacg ccagcaccag
     8881 cgcgcccagt ccgcccatag aatgaccaga gatagacttt ttggccgttg ccggaaaatg
     8941 attcatcact aaatccggca gctcgttgcg gatatagtca tacattttgt aatgttcatt
     9001 ccacggcgct tgcgtcgcgt tcaggtaaaa cccggcacct tgcccgagat cgtaacggtc
     9061 ggcatctgcg acatgactgc ctcgcggact ggtgtccggc gcaacaacaa taatgttgtg
     9121 ctcagccgcg taacgctgca cccccgattt agtaatgaaa ttctgctcat tgcaggtcag
     9181 gcccgaaagc cagtacagca ccggtaattt ttcacctgca gcttttggtg ggagatagac
     9241 gccgacattc atttcacatt tcagtgatgc ggaataatgc cgatacacat tttgccagcc
     9301 gccaaagctg gcatgttttt caatgagttc catcagtcat tatctcacgg ttaatccccc
     9361 ggacggctgc ccggagagag tattaagaac cccgtctgaa gtggacgggg taaaccagcg
     9421 ggaaatcagt aacgaattac ggttcgaatg gatttgcctt catgcatcag gtcgaaggcg
     9481 tcattaattt catccaggct catggtatgc gtgacaaacg gttccagatc gatatcacct
     9541 ttcatcgcat cttcaaccat acccggtaac tgggaacgac ctttcacgcc gccaaacgcg
     9601 gaacctttcc atacgcgacc agtgaccaac tggaatggac gggtggagat ttcctgaccg
     9661 gaacccgcga ccccgatgat caccgactga ccccagccgc ggtgcgcact ttccagcgcc
     9721 gcacgcatca cgttgacatt accgatgcat tcaaaggtat ggtcgatacc ccatttgttg
     9781 atatccagca ggacatcttt aatcggtttg tcgtagtcat tcgggttaat gcagtcggtg
     9841 gcaccgaagc gacgcgccag atcgaatttc ttcgggttgg tatcgatagc gataatccgt
     9901 cccgctttcg cctgacgcgc gccctgaacc actgccagac caatcgcgcc aagaccaaac
     9961 acagcaacag aatcccctgg ctggacttta gctgtgttgt gtaccgcgcc aataccggtg
    10021 gtcacgccac agcccagcag gcagacgtgt tcatggtttg cttctggatt aatttttgcc
    10081 agagacactt ccgcgactac ggtgtattca ctgaatgtag aacaccccat gtagtgataa
    10141 agcggctgcc cattgtaaga aaaacgggtg gtgccgtctg gcatcaggcc tttaccctgg
    10201 gtttcgcgaa ccgcaacaca gaggttagtt ttgccagaac gacagaactc acactcgccg
    10261 cactctgcgg tgtaaagcgg gatcacatgg tcgcctggtt tgacgctggt tacgccttca
    10321 ccgacttcaa ccacaacgcc agccccttcg tgaccgagaa ccaccgggaa tacaccttcc
    10381 gggtcatcgc cggagagggt aaatgcgtcg gtatggcaaa cgccggtatg ggtgacttta
    10441 atcagcactt cacctttttt cggtggtgca acgtcaattt caacgatttc cagcggttta
    10501 ccgggagcaa atgcaacggc agcacgtgat ttcatctctc gctcttcctc aatatggtaa
    10561 tagattcagc tatttaagat aggcacgaac cagttcaata gtgtcgtcaa cggattggct
    10621 gacttcgcgg ctgtagcagt catttcggtc aaacgtttcc cggatatggc tttcaagcac
    10681 ttctgccatc agcccattag ccgcgccccg aacggcagcg atctgttgga gtatggcacg
    10741 gcattcggca tcaccctcca gcgaccgttc cagagcatca atctgccctc gaatacgacg
    10801 aactcgagta aggacctttt tcttctcttc cggagtactg ggcatttcgc acctcatcat
    10861 ctgcacgcaa tatactatag gggggtatgc tatatgtcaa tgcatacccc cctatagtat
    10921 ataacaatgg agaataattg ttgtccgaag cctaaccagg tgaacggtcc ctcaagacag
    10981 atggggaccg tttatgtgct atttttatgc cgcatccgct aaaacaaaca tcccgtaagg
    11041 atggatttcg aggtaatagt gctcaccaat atcgggctgt aaacgggtgg cgtttacttg
    11101 taataaaatc tcctgtccat gccactctac ggtcacttcg tactgcggcc ccatgtaggc
    11161 gacatggcga ataacacaac gctgactctc ttcgccgtga ttgcttaagg tgatggcttc
    11221 cgggcgaacg ccgacggtgc cgctaccttg caccgggaaa tgcgccgcgc gcggcaggcg
    11281 ataaccgtag atatcgacat actcttcgct aaagttcgcc gggaacaggt tggcgtcgcc
    11341 cataaaactt gccataaagc gggaggctgg ctgacgatag agatcctgcg gtgagccaat
    11401 ctgcatgatg tgacctttat tcatcaccag cacagtgtcg gaaaccgcga aggcctcact
    11461 ttgatcgtga gtcacgtaga gcgacgtgat attaaactgc ttttgcagct cgcggatctt
    11521 atcgcgcatg ctgcggcgca ggttggcatc gaggttactt aacggctcat caaacagcag
    11581 cacctttggt ttgaggatca acgcgcgggc cagcgccacg cgctgttgct gaccaccgga
    11641 aatctggtcg acatagcggt cctcgaaccc ttccagatcc accattgcca gcgcctcttt
    11701 cacccgctgc tttacttcgc tacgcgacac gccgagcatc tttaacccgt agccgacgtt
    11761 ttcgcccagc gacatatgcg ggaacagagc gtatgactga aacaccatgc agatatcgcg
    11821 ctgctgaatg gaacgatgcg tgacatcttc gccatcaata aagatttgcc cttcactcgg
    11881 tttttccagc ccggcaacca gacgcaaaat ggtggttttg ccgcagccgg aagggccaag
    11941 cagcgtcacc atttgccctt gtgggatggt gagattgata ttgtcgataa ccgtattact
    12001 gccgaatcgt ttagtgacgt tgcgcagttc cacaaaattt ttctgactca tagtgcgctc
    12061 cattacgcct ggtttttggc tttagaacgg gagatacgtg cttctccgat cagccagtca
    12121 aagaggaaaa taatcgccag catcaccacg atcagaatcg aaccataggc gatggctacg
    12181 ccatattcgc cgtcttcaac gcggttaaga atgtaggcgg ttgccacgcg ggtgtccggc
    12241 gtgacgagaa agacgatggc gctgaccgtc gtgatggccc gcacaaagct gtagatcagg
    12301 gccgagagaa tggccggacg cagcagcggc agtaaaatgt gggttatggt tcgcaaggaa
    12361 cccgcgcgca ggctgagtga ggcttcatcc agcgatttgt cgatctgccc caggcctgca
    12421 atcccggcgc gaatacccac cggaacgtta cgcatcacca tcgagataat cacaattgcc
    12481 gccgttccgg tgagataaac cggagcgctg ttaaaggcaa gaatatacga aacgcccgcc
    12541 accgtacccg gtacggcaaa gcacagcatg gtggtgaact cgatggtctt tttgcctttg
    12601 aattgttgac gcaccaccac ccaggcgatg agcaggccaa agatagcggt aatgggagcg
    12661 gcaatcccgg cgtagagcag ggtatccagc agtgaaggcc acgcgccgtc gctcattccc
    12721 tgaccaaata gcttgataaa gttatccagc gtcagggtgt agtccacgcc ccagttcacc
    12781 gtgaagctgc cgtagaaaat gctgccgtag agcagggcgt taaaggcaat ccatatcgca
    12841 agtatggcga ccacgctcca caccagggtg accggaagcg gctggacgtc gccacggtag
    12901 gatttgccgg aaaccgtgac gtaagagcgt ttaccaatcc acatatactg tatgcagaac
    12961 accagcagcg agaacagcag caggaaagcg ccaagggtgc tggcggcctg ataatccagc
    13021 tgcgagccgg taatatagaa gtaaatttgc gtcgccagta cgtcgaagtt accgccaagt
    13081 accagcgggt tactgaaatc agccagtgac tgtaccacga cgattaaaaa ggcattggcg
    13141 agcgcgggct tcaacaacgg cacaaacacg ccgttaaagg tttgccagcg gctggcgcgc
    13201 agggtgtagg aggcctcttc cagcgaggga tgaatggttt taatggcccc gtcgaggatc
    13261 ataaatgcca ttggcgtgaa tgccagaacc tgtgccagcc agatcccggt aaaaccgtac
    13321 agccagttgg tgttggtcag cccaaaccac tccaccataa attccgtaac atagccggaa
    13381 cgccccatca tgagcgtcac gcctaagccg accacaaacg gtggcgtaac gatgggcaaa
    13441 atggaaaaga tgcgaccaat aatggcgctg cgtttagcga tgcgcgtggt ataaatcgcc
    13501 agcaccaggc cgaagaaagc acagcctatc cccaccgcta tcgacaagac gatagagttg
    13561 acgatcacct gaacaatatg tgcctgcgag agcacggtca taaacgccag cggggcgagc
    13621 tctcccgcgt cgttggtgaa catcgggata aaaatggcga tgctcggcca gacgataaaa
    13681 atgccaatca gcgccacaat cgcaatcagt gagccgatca cgaagcggtc gccgcccagc
    13741 cattcgaggc gggttaaggc cagcgtcata atcgcgccta gttcgacaaa cagcacgatg
    13801 gcggcgtagc ctaaaccgcg tccagccacg gtggcgctga tgacgataaa cgccatacag
    13861 agcaatgccc agccggcatc gaaataatga cgactacgct gttcgcgcgt ggcctgacga
    13921 aacgggcgga ccagcaacag gctcgggagt aaataccaca gccagctgat attaaaatgc
    13981 gaccagccgt acgccccgag gatctcatca ctcgtggatt ccagcaggcc ataatcgagg
    14041 ctccagctcg gcaacaggac aaatgccagc cagcccagca aaacccaaag gaaaatcgca
    14101 tcccgctttt tcacgggatg gagtgcaagt gtctgtgaca tagcagttcc ggtatggttg
    14161 gccggggcag gcgtctgcgc tgcacccggc aaatgcgcct aatgcgggtt tatttaccca
    14221 tcttcacttc gctgacccat ttattaatca atgccttacg aacatccgtg gaaccatatt
    14281 tatccatgtc gtagttgatt aatttcaggt cgtcgagctt cagcgaattt ggcgacgttt
    14341 cggcggtggt attagtcagg atctgataag acttcccttt cttccacgcc agttcctgag
    14401 cttcttttga taatacccag tccacaaaca gtttggcgtt gtcgaggttg cgcgcgcctt
    14461 tcagaatgct gacgccgcca atttcgtagc ccgtaccttc acacggggaa atcaactcca
    14521 gcggcgcgcc ctgttctttt tccagcgagt agtcatgcag gaagccaata ccaatcgccg
    14581 tttcgccacg ggcggcgtta cgtgccgggg ctatgcctga tttggtgtac tgagagacgt
    14641 tggcgttcag ttgttttaga taatcaaagg cctgatcttc cccccacagc tgcgcaaaag
    14701 tagccagggc ggtgtaggca gtaccggaac tttgcggatc ggcaatttga atttcgcctt
    14761 tatattccgg tttggtgaga tctttccagc acttcggcac cggcaggttt ttctctttca
    14821 gacgatcggt gtttacgcca aaaccgagga tccccacata aacggcagag gacagattcc
    14881 ctttcacttt tgccggatcg cggaatttct ccatgatctg ctcaaggtta ggggatttat
    14941 acgcctgtag caggcccatc tcgccagcct gcgattgcgg gtctaaagtg ccgccgtacc
    15001 agacgtcagc ctgggggttt ttcttttcag catcgacttt tgccagggtg cttccggaac
    15061 cgttgcggat aaacgaggtt tttacatcat atttctcgcc aaacgctttg gtttcggcct
    15121 cacacatttc attggtggcg ctgcaataga ccaccagccg gccttttgcc tgcgcggcac
    15181 ccgttaaagt cgccagcgca acggcagaag caatcagagt agaggtaaag gtcattttca
    15241 ttgttttatc cttcgagcgt gagtggtggt gatgcccgcc atcagtaacg gcatcagcag
    15301 taaacccata agtactgcgg cgaccgagag taaactgaac attcccggcc agccgtagcg
    15361 ttcgatgacc agcgacagcg gccagcctgc cagcgccgcg ccaaggtaag cgaacaggcc
    15421 gagaaatccg gtgattgaac ccgccgcagc tttgtgccca cactccaccg ccgcaagtcc
    15481 aatcagcatt tgtgggccaa agacgaaaaa gcccacggta aaaaagcaca ctgccagcag
    15541 agcgtagtga tgaaccggtg ccagccacag tgcggcgact gacaccatca accccaacgt
    15601 gaacagcaaa atcattggcg ctcgttgccc gctaaacagc aaatccgatc cccatccggc
    15661 aaataacgcg ccgagtaagc cgcccacttc aaacagcatt accgtggcgt tggcgctgag
    15721 caggttgaca ccgtggcttt ccgtcagcca gatattgccc cagtcgttaa gcgcaatccg
    15781 gatcacataa accagtacat atgagacgcc cagtagccag atcagcggat tttgcagcat
    15841 ggtggtgcgc aacatttgcc acagccccat cggcggactt tgttgttcct gacgtaactc
    15901 cagcggatcg tgacgccagt gaccaactgt cggtaatccc tcttcctggg gcgtgccttt
    15961 gagttgtagc gttaaccaga tccctaatgc catgctgatt atccccggtg tcagcatcgc
    16021 ggcctgccag ccccaccagt gggcggcaaa ggcgctaatc agcggaataa tcgctccgcc
    16081 aatgttgatc gacatattcc agcagcccca ccagaaaccg cgctcattgc gtgagtacca
    16141 gtgggtcagc aagcgggcac acggtggcca tccccagccc tgaaaaaaac cgttcagtgt
    16201 ccagaccaca agcagaagcg taagtgattc gccaaaggca aacaccacgt tcaataaccc
    16261 ggtggcgaac aagccgacgc ccataaaacc gcgttgtccg tggctgtcat gccacagtcc
    16321 ggcggtaaat ttcgacaatc cgtagctgag ataaaacagc gaacccagca ggccaatgtc
    16381 gcctttatcc agtccgaggt cggtttgtag cgccggaagg acgtaattca cgctttttcg
    16441 cgtcagataa aatgcggcgt agccaataat catgcacatc agcaatcgcg gacgtaatgt
    16501 ccggtagcat tgattaattt ctctcgctga acgcgcgtgc atggttgtct ccatgttgct
    16561 gactatatga aaatgaaagt gaaaggggat gagagaaaat ccgggatacc taagactttt
    16621 tcctggttat ccggcgattt gttgcaaaaa tgtgggcaag ttaacaatta cccgcgtgcc
    16681 gtgctgtttt tcgagcgtta agtcgccgcc cagggcgctg acgcgctcac gcatcccctg
    16741 aatgccaaat ccagccattt tcccgctggc gatccccaca ccgttgtcgc tgacttccag
    16801 atgcaatacc tctccctgct ggcgcaggat aatcgtcact tcgctggctt cggcgtgttt
    16861 acagatgtta ttgagcaact cctgcaataa ccgatacaag gtaaagcgca ccgtttcgtt
    16921 ttctggtgtg gagttcagtt gataggcaaa ctggcaatga atgccgcgtt cactaaaggc
    16981 aaattcatta atcagatgca gcaacgcttc gcgaaatgtc agttcatcaa gcgcgggagg
    17041 ccgcaactgg cgcagcaatt ggcgggtgga gagatgaatg cggcgggcga gctcattgat
    17101 ttggctggct gcggattgaa tttgcgccgg atcacgcgcc cgcttcacca gttgcgactg
    17161 gatctgaatg gcggtgatgt tctgaccgat ttcgtcgtgc agctctcgcg ccaggttttt
    17221 tcgcgtgtcc tcctcggtgt ggatcagctt ttccgccaat gcccgccgcg ctgccagctc
    17281 ggcctccagc cgttgtcggt aatggtgcag gtttagcgcc agatgttgct ggcgactgat
    17341 ggcaatgccc aggccaattc ccagcaaggc ctgggtcgcg aggaaaatct ccagctccac
    17401 caggttacta aagcccacgc caatctggcg ggcgatggtg atcatcatgc tgcccaaaag
    17461 cccggagagt acgccacctt gccagccaaa tttccacgcc atcaccacat tgggcaaaaa
    17521 gaccacgatc aacagcagac gttcaatttc aggtgacagc accatctgag tgccgatacc
    17581 aataataaaa aacaggctac accagatgat taaagaggta cgcaaggggg gattatgcgt
    17641 atcgagtccc aggagctggt agcgatgctg ctggcgcaga aactcaaaga taagatagac
    17701 aaacggcgtc agtaatacgc cgccggtaaa tgaggtcaaa cccggtagca ttattgggcc
    17761 tgacataaaa ggggagagca gggcggtatt caacaatgaa gctgccgtga cagtggctaa
    17821 cagcaacgtc agacgctgcc agtaaagtgg aaaacggtgc cagaagtttt gcgcaaggag
    17881 ggcaggaaac aggctaataa ccggcgttgc cagcatgaga taaccgttca atagctgttc
    17941 actatggagc cagaacatca tcagcaaagg ggggagcacc aacgcaggcc agtagcggcg
    18001 agagagcaaa ataacaaggg cgagataaac tccctgcggg agaaacaaca ccgcctgttg
    18061 accgttattg gtcagataaa agctgagcgt ccacagcatc agccagccgg ttccccaggc
    18121 tagcacaata aacagcaaaa tcacccaatg acgaagattg cgcgacatta atgccccgcc
    18181 agcagatggt ggtcgagcgc aaaatgtacc agttcaatgg tgctatggca gttcagtttt
    18241 cccagcacat tggcgcgatg cacatgtacc gttttatgac tgagttcgag tttaaaggcg
    18301 atctctttaa cgctgtctcc tttgaccagt aactcaaaca cttcacgttc tcgtggggtg
    18361 agaatttcca gcgcctgggc gggttgcccg ccgccgcgta aagcccgaat cgcatcggca
    18421 cagagataat gcccgcccag cccgaccgag cgcaccgcct gtactaactc ttccggccca
    18481 cagcgtttgg ttaaataccc gctggcaccg gcatccagcg cgctttgcac aaagattggc
    18541 gcgtcataaa tgctaagaat aatggcgcga aattgcggtt tttgtgcacg caggcgctta
    18601 agcaaactga ggccgttttc gtccggcatg gcaatatcaa tgacggcaac attgacgtca
    18661 tcacggatta atgccgacca tgcctgcgcg gcactactgt actgaccaat gacctccaga
    18721 tcgtcttcca gagagagtaa ttgtgcgaag ccagagcgca ccacaacatg gtcatccacc
    18781 agcactaccc gaatcatact tttcttctct tattatctaa ttctcctatc atgcatcacg
    18841 gcgcggcgct ggcaatcaac cagcgacaga tatggaatgg atgtaacaaa atggcaataa
    18901 tttgccggag cgcgcacatc ttatctggct tgtgaaccgg cgggatacgt tcgccaaaac
    18961 cgcaaaatgc gcaaactcgc taaccattgc cgattttttt ctaaaaacgc tttgttctta
    19021 acaagacctt ttaattcctt tatcaaactg acccgcaacg aaatactgct cccataacaa
    19081 gacaggggag cagacaatca tggcaatttc atcgcgtaac actcttcttg ccgcactggc
    19141 attcatcgct tttcaggcgc aggcggtgaa cgtcaccgtg gcgtatcaaa cctcagccga
    19201 accggcgaaa gtggctcagg ccgataacac ctttgctaaa gaaagtggag caactgtgga
    19261 ctggcgcaag tttgacagcg gagccagcat cgtgcgggcg ctggcttctg gcgacgtgca
    19321 aatcggcaac cttggttcca gcccgttagc ggttgcagcc agccaacagg tgccgattga
    19381 agtcttcttg ctggcgtcaa aactgggtaa ctccgaagcg ctggtggtaa agaaaactat
    19441 cagcaaaccg gaagatctga ttggcaaacg catcgccgta ccgtttatct ccaccaccca
    19501 ctacagcctg ctggcggcgc tgaaacactg gggcattaaa cccgggcaag tggagattgt
    19561 gaacctgcag ccgcccgcga ttatcgctgc ctggcagcgg ggagatattg atggtgctta
    19621 tgtctgggca ccggcggtta acgccctgga aaaagacggc aaggtgttga ccgattctga
    19681 acaggtcggg cagtggggcg cgccaacgct ggacgtttgg gtggtgcgca aagattttgc
    19741 cgagaaacat cctgaggtcg tgaaagcgtt cgctaaaagc gccatcgatg ctcagcaacc
    19801 gtacattgct aacccagacg tgtggctgaa acagccggaa aacatcagca aactggcgcg
    19861 tttaagcggc gtgcctgaag gtgacgttcc ggggctggtg aaggggaata cctatctgac
    19921 gccgcagcaa caaacggcag aactgaccgg accggtgaac aaagcgatca tcgacaccgc
    19981 gcagtttttg aaagagcagg gcaaggtccc ggctgtagcg aatgattaca gccagtacgt
    20041 tacctcgcgc ttcgtgcaat aaaaggaggc gcagatgctg caaatctctc atctttacgc
    20101 cgattatggc ggcaaaccgg tactggaaga tatcaacctg acgctggaaa gcggcgagct
    20161 actggtggtg ctggggccgt ccggctgcgg taaaaccacc ctgctgaatt tgattgccgg
    20221 ttttgtgcct tatcagcatg gcagcattca actggcgggt aagcgtattg aggggccggg
    20281 agcagagcgt ggcgtagttt ttcagaatga agggctacta ccgtggcgca atgtacagga
    20341 caacgtggcg ttcggcctgc aattggcagg tatagagaaa atacagcgac tggaaatcgc
    20401 gcaccagatg ctgaaaaaag tggggctgga aggcgcagaa aaacgctaca tctggcagct
    20461 ttccggtggt caacgtcagc gggtggggat tgcccgcgcg ctggcggcga atccccagct
    20521 gttattactc gacgaaccgt ttggtgcgct ggacgccttc acccgcgacc agatgcaaac
    20581 cctgctgctg aaactctggc aggagacggg caagcaggtg ctgttgatta cccacgatat
    20641 agaagaagcg gtgtttatgg cgactgaact ggttctgctt tcacccggcc ctggccgcgt
    20701 gctggagcgg ctgccgctca actttgctcg ccgctttgtt gcgggagagt cgagccgcag
    20761 catcaagtcc gatccacaat tcatcgccat gcgcgaatat gttttaagcc gcgtatttga
    20821 gcaacgggag gcgttctcat gagtgtgctc attaatgaaa aactgcattc gcggcggctg
    20881 aaatggcgct ggccgctctc gcgtcaggtg accttaagca ttggcacgtt agcggtttta
    20941 ctcactgtat ggtggacggt ggcggcgctg caactgatta gcccgctgtt tttgccgccg
    21001 ccgcaacaag tactggcaaa actgctcacc attgccggac cgcagggatt tatggacgcc
    21061 acgctgtggc agcatctggc agccagcctg acgcgcattg tgctggcgct actcgcggcg
    21121 gtgttgatcg gcattccggt cgggatcgcc atggggctaa gcccgacggt gcgcggcatt
    21181 ctggatccga taatcgagct ttatcgtccg gtgccgccgc tggcttattt gccgctgatg
    21241 gtgatctggt ttggtattgg tgaaacctcg aagatcttac tgatctattt agcgattttt
    21301 gcaccggtgg cgatgtcggc gctggcgggg gtgaaaagcg cgcagcaggt tcgcattcgt
    21361 gctgcccagt cgctgggtgc cagccgtgcg caggtgctgt ggtttgtcat tttgcccggc
    21421 gcgctgccgg aaatcctcac cggattacgt attggtctgg gtgtgggctg gtctacgctg
    21481 gtggcggcgg agctgattgc cgcgacgcgc ggtttaggat ttatggttca gtcagcgggt
    21541 gaatttctcg cgactgacgt ggtgctggcg gggatcgcgg tgattgcgat tatcgccttt
    21601 cttttagaac tgggtctgcg cgcgttacag cgccgcctga cgccctggca tggagaagta
    21661 caatgagtga acgtctgagc attaccccgc tggggccgta tatcggagca caaatttcgg
    21721 gtgccgacct gacgcgcccg ttaagcgata atcagtttga acaactttac catgcggtac
    21781 tgcgccatca ggtggtgttt ctgcgcgatc aagccattac gccgcagcag caacgcgcgc
    21841 tggcccagcg ttttggcgaa ctgcatattc accctgttta ccctcatgcc gaaggggttg
    21901 acgagatcat cgtgctggat acccataacg ataatccgcc agataacgac aactggcata
    21961 ccgatgtgac atttattgaa acgccacccg caggggcgat tctggcagcg aaagagttac
    22021 cttcgaccgg cggcgatacg ctctggacca gcggtattgc ggcctatgag gcgctctctg
    22081 ttcccttccg ccagctgctg agcgggctgc gtgcggagca tgattttcgt aaatcgttcc
    22141 cggaatacaa ataccgcaaa accgaagagg aacatcaacg ctggcgcgag gcggttgcga
    22201 aaaatccgcc gttgctacat ccggtggtgc gaacgcatcc ggtgagcggt aaacaggcgc
    22261 tgtttgtgaa tgaaggcttt actacgcgaa ttgttgatgt cagcgagaaa gagagcgaag
    22321 ccttgttagg ctttttattc gcccatatca ctaaaccgga gtttcaggtg cgctggcgct
    22381 ggcaaccgaa tgatattgcg atttgggata accgtgtgac tcagcactat gccaatgccg
    22441 attacctgcc acagcggcgg attatgcatc gggcgacgat tcttggggat aaaccgtttt
    22501 atcgggcggg gtaatacgag agtggacggt cctctcgccc ccttggggag agggttaggg
    22561 tgagggggcg ttcaccgtgc tttcaacagg ttaactcccc ctttctgaga ggaaacaaaa
    22621 ttaacgcaga atcttcttct cagccaaatc cagcgcaaag tagctgaaaa tcagatccgc
    22681 acccgcacgc ttaatcgaac ctaagctttc gagcacgact ttctcttcat ctatagcacc
    22741 cgccagcgcg gcgaacttaa tcatcgcata ctcaccgctc acctgatacg cgccaatcgg
    22801 caattcagta cgttcacgca gctcacgcac gatgtcgagg tacgctccag caggtttaac
    22861 catcaggcag tctgcgccct gggcttcatc cagcagtgac tcacgaatcg cctcacgacg
    22921 gttcattggg ttcatctgat agcttttgcg gtcgcctttt aatgcgcttc cggcagcttc
    22981 acggaacgga ccataaaagg aggaggcgaa cttggtcgaa tacgacataa tcgccgtatc
    23041 tttaaagcca gcagcgtcca gcgcctggcg aatcgcctgt acctggccat ccatcgcggc
    23101 ggaaggggcg atgaagtctg cacctgcagc agctgcaacc acggcttgct tgcctaaatt
    23161 ttccagagtc gcgtcgttgt cgacgccatg ctcgcacagc acaccgcagt gaccgtgaga
    23221 agtgtattca cagaagcagg tgtctgacat gacaatcatt tctggcaccg tctgcttgca
    23281 gatgcgcgac atacgcgcta ccagtccatc ttcccgccag gcatcgctgc cggtttcatc
    23341 ggtatggtga gagatgccga aagtcatcac ggaacgaata ccggcgttgg cgatgcgttc
    23401 aatttcgcgt gccagatgtt tctctggaat gcgcatcacg ccaggcatgg cttcaacggc
    23461 tttgtagtcg tcaatttctt cttcaacaaa gatcggcaac accaggtcgt taaggctaag
    23521 tgttgtctct tcaaacatag cgcgcagcgc aggagatttg cgcaggcgac gagggcgttg
    23581 gattaagtct gtcatggtct gcctgatgtt tgtggaatta aggtgcacag tatacctgaa
    23641 gcggggctgg agtgttttac gaaagttgtc acgatgttga caagaagaga atgaaagaga
    23701 gaccagggca t
//
LOCUS       DBFIFS010000051        23675 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.051, whole genome shotgun sequence.
ACCESSION   DBFIFS010000051 DBFIFS010000000
VERSION     DBFIFS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.051"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            2..1630
                     /gene="yqiK"
                     /locus_tag="N7A07_001884"
     CDS             2..1630
                     /gene="yqiK"
                     /locus_tag="N7A07_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU2476559.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLKIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTRI
                     LAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            1869..1928
                     /locus_tag="N7A07_001885"
     CDS             1869..1928
                     /locus_tag="N7A07_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2476560.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2245..2304
                     /gene="ibsE"
                     /locus_tag="N7A07_001886"
     CDS             2245..2304
                     /gene="ibsE"
                     /locus_tag="N7A07_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU2476561.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(2424..3857)
                     /gene="hldE"
                     /locus_tag="N7A07_001887"
     CDS             complement(2424..3857)
                     /gene="hldE"
                     /locus_tag="N7A07_001887"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU2476562.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3905..6745)
                     /gene="glnE"
                     /locus_tag="N7A07_001888"
     CDS             complement(3905..6745)
                     /gene="glnE"
                     /locus_tag="N7A07_001888"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU2476563.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(6768..8069)
                     /gene="ygiF"
                     /locus_tag="N7A07_001889"
     CDS             complement(6768..8069)
                     /gene="ygiF"
                     /locus_tag="N7A07_001889"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU2476564.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8311..8931
                     /gene="ygiM"
                     /locus_tag="N7A07_001890"
     CDS             8311..8931
                     /gene="ygiM"
                     /locus_tag="N7A07_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU2476565.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8995..10233
                     /gene="cca"
                     /locus_tag="N7A07_001891"
     CDS             8995..10233
                     /gene="cca"
                     /locus_tag="N7A07_001891"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU2476566.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(10414..11235)
                     /gene="bacA"
                     /locus_tag="N7A07_001892"
     CDS             complement(10414..11235)
                     /gene="bacA"
                     /locus_tag="N7A07_001892"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU2476567.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11326..11694)
                     /gene="folB"
                     /locus_tag="N7A07_001893"
     CDS             complement(11326..11694)
                     /gene="folB"
                     /locus_tag="N7A07_001893"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU2476568.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11800..12417
                     /gene="plsY"
                     /locus_tag="N7A07_001894"
     CDS             11800..12417
                     /gene="plsY"
                     /locus_tag="N7A07_001894"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU2476569.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12430..13362)
                     /gene="ttdR"
                     /locus_tag="N7A07_001895"
                     /gene_synonym="dan"
     CDS             complement(12430..13362)
                     /gene="ttdR"
                     /locus_tag="N7A07_001895"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU2476570.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13569..14480
                     /gene="ttdA"
                     /locus_tag="N7A07_001896"
     CDS             13569..14480
                     /gene="ttdA"
                     /locus_tag="N7A07_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU2476571.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14477..15082
                     /gene="ttdB"
                     /locus_tag="N7A07_001897"
     CDS             14477..15082
                     /gene="ttdB"
                     /locus_tag="N7A07_001897"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU2476572.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15131..16594
                     /gene="ttdT"
                     /locus_tag="N7A07_001898"
     CDS             15131..16594
                     /gene="ttdT"
                     /locus_tag="N7A07_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU2476573.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16637..17650)
                     /gene="tsaD"
                     /locus_tag="N7A07_001899"
     CDS             complement(16637..17650)
                     /gene="tsaD"
                     /locus_tag="N7A07_001899"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU2476574.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17888..18103
                     /gene="rpsU"
                     /locus_tag="N7A07_001900"
     CDS             17888..18103
                     /gene="rpsU"
                     /locus_tag="N7A07_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU2476575.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18214..19959
                     /gene="dnaG"
                     /locus_tag="N7A07_001901"
     CDS             18214..19959
                     /gene="dnaG"
                     /locus_tag="N7A07_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU2476576.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20154..21995
                     /gene="rpoD"
                     /locus_tag="N7A07_001902"
     CDS             20154..21995
                     /gene="rpoD"
                     /locus_tag="N7A07_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU2476577.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22074..22580)
                     /gene="mug"
                     /locus_tag="N7A07_001903"
     CDS             complement(22074..22580)
                     /gene="mug"
                     /locus_tag="N7A07_001903"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU2476578.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22705..22780
                     /locus_tag="N7A07_001904"
     tRNA            22705..22780
                     /locus_tag="N7A07_001904"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22738..22740,aa:Ile,seq:cat)
     gene            complement(22834..23598)
                     /gene="nfeF"
                     /locus_tag="N7A07_001905"
                     /gene_synonym="yqjH"
     CDS             complement(22834..23598)
                     /gene="nfeF"
                     /locus_tag="N7A07_001905"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU2476579.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         5697 a         6508 c         6097 g         5373 t
ORIGIN      
        1 gatgtttacc gcgattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag
       61 gctctatcgt cgcgcttcgg cagagcaagc atttgtccgt accggcttag gggggcaaaa
      121 agtggtaatg agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa
      181 tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga
      241 tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg gtaaaacctt cagtagaagg
      301 gattgccacc gctgcccaga cgctggggca acgcaccctg tcgcctgaag acttacgtat
      361 gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcgcaaa tgaccatgca
      421 tgagttacag gatacccgcg agaacttcgt gcagggagta caaaataccg tagctgaaga
      481 tctgtcgaaa aacggcctgg aactggaaag cgtttcactt accaacttta accagacctc
      541 gaaagaacat ttcaatccta acaatgcctt tgacgccgaa ggtttaacca aactgactca
      601 ggagacggag cgccgtcgcc gcgagcgtaa cgaagttgaa caggatgtag aagttgcggt
      661 gcgtgagaaa aaccgtgatg cactttcgcg caagttgaag attgaacaac aagaagcgtt
      721 tatgacgctt gagcaggagc agcaggttaa aacccgtacc gctgagcaga atgcgaaaat
      781 tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc tggctgaacg
      841 acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa aagttgaagc
      901 tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgctaacca
      961 gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc
     1021 taaccttgca ctcgcagaag cagtaagcgc ccaacaaaac gtagaaacca ctcgccagac
     1081 agccgaagcc gatcgtgcca agcaggtggc gctgattgcc gcagcacagg atgcagaaac
     1141 caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag agatgcaggc
     1201 ggcggcgatc gttgagttag ccgaagctac gcgtaaaaaa ggcctggcgg aagcagaagc
     1261 acaacgtgcg ctgaacgatg ctatcaacgt actttctgac gaacaaacca gccttaaatt
     1321 caaactggcc ttgttgcagg cgctacctgc ggtaatagag aaatccgtgg aaccaatgaa
     1381 gtccattgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggtg gtactgcggg
     1441 ggatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat tatcagccgc
     1501 actctcttac cgcacacagg caccactgat tgactccttg ctcaatgaaa ttggcgtttc
     1561 aggcggctca ctggctgcat tgacttcact cttaacccca acaactcccg tcgccgaaaa
     1621 cgtagaataa attttcagct atgaacatcc ctggctcaaa aagccgggga ttttttatat
     1681 ctgcgttccg ctaaaaggtg caaatgatca ggctgttgca gcgttttgcg tgattgctcg
     1741 gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca tcaacaatcg
     1801 gggagtaagc aaagggctga aacgggaaag cccctcccga ggaaggggcc ttacagaagg
     1861 aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta agtttcgcag
     1921 cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc ctttacttta
     1981 ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctgg cccaaaaagc
     2041 cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac gttgcagcgt
     2101 tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg cggggtccgg
     2161 cgctcatcaa caatcgggga gtaagcaaag ggctgaaacg ggaaagcccc tcccgaggaa
     2221 ggggccttat agaaggaaag ggttatgatg aagctcgtca tcatactggt tgtgttgtta
     2281 ctgttaagtt tcccgactta ctaacaactc atcagagggg ggagaaatcc tcccttaccc
     2341 ttgttccttt actctaggtt gaaaagacaa cagcgtcaat aggcctgcca tgtacgaagc
     2401 gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt cttgataatg
     2461 ttagtggtcg agcaaccgtc ttcaaagttg agcaccaaca cttcgccgcc gttggcccag
     2521 acttctttac tcccggcgat ctcttccggt ttgtagtcac cgcctttcac cagcaggtct
     2581 ggcaggatcc cggcgatcag acgctgcggc gtgtcttctt caaacgacac tacccagtcg
     2641 accgcttcca gtgcgcccag cacaatcata cgctgttcga gtgggtttac cgggcgggaa
     2701 tcccctttca gccgtttggt ggaggcatcg ctgttgacgg caacaatcaa gcggtcaccc
     2761 agcttgcggg catttgccag ataagagacg tgcccggcat gtaggatatc gaagacaccg
     2821 ttggtcatta ccactttttc accacgttta cgcgctgcca ctacagccag cttcagttcc
     2881 tcttcggtca tcacgccaaa gccggtatct gcgcgtccac gtacagcgtt ttccagctcg
     2941 atcggcgaaa ccgtggacgt ccccagtttg ccgaccacca caccagccgc cgcattggca
     3001 aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag gacgccaatc
     3061 accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgagttgg catatgcagc
     3121 ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac taacagagcc
     3181 gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc ttcggtctta
     3241 catttaccga caacagcttc aaattccgag agattcggcg ttaacagcgt agcgccgcgg
     3301 tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacacccgc tttacgcgcc
     3361 agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga aagcaccagc
     3421 gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg atcaacacct
     3481 tcgaaacctt cttcaaaatc cagacggatc agctgttggt tgcgggaaag tacccgtaat
     3541 ttggtaatgg tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt gacgtcggcc
     3601 agagatttac tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac caggcgtgca
     3661 ttagcaccga gagaagcgat attcatcgcc acgttagccg cgccgcccgg acgttcttcg
     3721 atggtattca ctttaaccac gggcaccggc gcttccggcg agatacgact ggaggggccg
     3781 taccagtaac gatccagcat cacatcacca accaccatca ctcctgcacg ttcaaactct
     3841 ggcagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc gataatacca
     3901 tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagttca cgctctgcgg
     3961 tgaagcaatc ctccggcaca tggcccggca attcctgtaa tgccagatga tgaagttcat
     4021 cgcgaagcgt agtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt
     4081 tttgcgccag tagttccaga atgcgcacgt tgtctgacca gcgcgttaac ttcggttttt
     4141 catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta attccccctt
     4201 catcagcttt gatatcaaag cgatcgcgat gtttattgcc gagatgagcg cgcattttct
     4261 cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc gtcataatct
     4321 cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact acacgcgcac
     4381 gcaccagcgc ctgatgctcc cacgtccagg cctcgttttt ctgataatcg gcaaatgctt
     4441 ctgtggaggt aaccagcatt cccgccgccc cggacgggcg cagccgcgca tccacttcgt
     4501 acaaaatgcc ggaagaggtt cgcgtgctga acaggtgcat aatgcgttgc gccagacgca
     4561 gataaaactg tcgtccgtcg atttcccgct cgccgtcagt catcgcatcc attgggcaat
     4621 catggaggaa gatcaggtca agatcggaac tgtagcctaa ctcccagccg cccagcttgc
     4681 cgtagccgac cactgcaaaa ccacgccctt cgcgttcgtt caggtgattc ggcttaccgt
     4741 agcgggcaac catttgaacc cacgcctgct gaacgacggc atctatcatg gcttccgcca
     4801 gccaggttaa gtgatcgctc actttcatca ccggtagcgt accggcgata tccgctgcgg
     4861 cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt tgctcttcgt
     4921 catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca tcggtcgccg
     4981 tcggctggta aagggtgttt ggatcgagca attcatccag caataatgga taacgcgcca
     5041 gctggctggc aatcatcggc gacgctgcac acagagaaat caaatgttta agcgccgcgg
     5101 ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccgcc agcaaggcgg
     5161 taatgcgcaa cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt agcagatgcg
     5221 gcatcagatg gtcgagcacc tgacgcccgc gtggcccgat tgtgcgctta tccagctctt
     5281 tgcggaaatc ggcaatcagc gtcagcacct gtttgcgatc gtcctcgcta agatgcgcca
     5341 gcactggtgt agtgtcatct tcctgcaacg catcctgcca cagctcgcgc cactgttccg
     5401 acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta aacacccggc
     5461 gcacattggt catatgtgcg gtcagcgccc cggtcaattg cggccagtca gcaaagtcca
     5521 tcgcccacgc cagccgcgca cggttaagct catcagaagg aagcgtctgg gtttgttcgt
     5581 cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatacgcc actcgcaatt
     5641 gttcagcatc gttttcagaa agcagatgca gcgcggcaat gacgctgagc gttggcagta
     5701 aagagcgcga ctgcagcgac ggttcgcgcc cgccgcgaat gagctggaac acctgaacga
     5761 taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc aaaccacgtc
     5821 gacgcacttc acgggcaatc attcctttca tgttgcgcag cgactgaatc acgctgaaat
     5881 cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta gcatagacgc
     5941 cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag tcgcgcccct
     6001 gctcctggta ataatcttcc aacgcggcaa agctcagcac cagcgggcca ctttcgccaa
     6061 acggacgcag ccgcatatcc acgcgataga cgaagccatc ctgcgttggt tgatccagca
     6121 ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt tcccagcgtc
     6181 caccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatca gaggagaaat
     6241 tcagttcccc gccgcccagc ttacccatgc ctaaaatcag cagcggttgc gcttcgccct
     6301 gcgcattgca cggcgttccc cactcgcggc agcaggcgtc atatagccag tcacgcgccg
     6361 caacaatcag cgtctccgcc agatagctga gctgctgcaa tatgctctct tcagtaacca
     6421 gtgccagcgt ttgcgcccag gcgatacgta ccatgatgcg ccgacggaac agccgcagct
     6481 cgcgcattaa cccggcttcg tcactcacat taatgagcgc ctcctgcaac catgcaacgt
     6541 aatgctgcca ttcgtcggcc tgcggcggtt ggctttccag ttccgtcagc cactctggat
     6601 gcgcacttat gctgtcctgc acaaaatcac tgaaagtaag tactgacttc gcctgtgcac
     6661 taagtgattc ctcggctaaa ggctctggca gccgctcaac aatggtctgc cagtactgct
     6721 gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta acgttttccg
     6781 ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc aatttcgatg
     6841 cgttgcccgg tcgcaatagc gtgatgcagc ccctgccagt tctccagcca ggcttgcgcg
     6901 acgacaggat catagtaacc cgccagcaac agtattgagt caatatcacg cgtcaggcgt
     6961 ggcaactggt cacggtagcg atcgcctaac ggctggcaga aaacgctttt cagttcagcg
     7021 gcatggcggg aaagatggat atcggcaaag cgtttgaagg agtcgctgat tttgccctgc
     7081 gctttggcat ctaaaaatgg ctgccatgct ttgcttacca accattcggt caacgccagc
     7141 ttcgccattg ccgtttcggt agagtagacc gccgtcacgg cagaaaccgc agaagcaatg
     7201 gtcgcttcgc attgggtcag caaatcacgt aagtgagtgc tcgctttacg cggcacaata
     7261 ccaccgaaca gcatcagcgt atgacggacc aagctaatgg ctgccagcac ctgttctttc
     7321 gccgcatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc taacgccagc
     7381 tcgagcgccg cttccagccc ctgttccaca tcggcttttg ccgcaacatg caaaatggtg
     7441 gtcggtttga tttcacgcgc cggattgccc tgcgccagat gatagccacg cgccgctttg
     7501 ctcaggctgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag tttcagcacc
     7561 gcgcgcgtgt cgccgctaag cagctccagt tccagctcac agataggttc agcaaattcc
     7621 cccgctttca cctcaccctg atcgagagcg atttcaattc ggctgccatc gaccgccacc
     7681 agccattttt cgcgataaaa atcggtgctg aacagcggtt gtacgcggga agcgagatcg
     7741 gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc gagcgtcggt
     7801 tcgctcaacg ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca
     7861 actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc catatcgtgc
     7921 ccacgcagcc agttatccgg cgtttcgtag taaatattca gtaactgcac ggggtcatgg
     7981 tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcgct gtgattaaca
     8041 ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg tcacataagt
     8101 gacgatgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa
     8161 ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca agacctgaca
     8221 cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca ctactatcgt
     8281 tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat cggattaact
     8341 ttactcgcac ttagcgcgac tgccgtctca cacgctgaag aaacgcgcta tgtttctgac
     8401 gaactgaata cctgggtccg tagcggtccg ggagatcatt atcgcctcgt gggcacggtt
     8461 aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta tgcccaggtg
     8521 aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac tgagccaagc
     8581 ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga taaactcacc
     8641 aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagc
     8701 gacagcgtga tcaacgggtt aaaagaagaa aatcagaaac tgaaaaacga gctgattgtc
     8761 gcgcagaaaa aggtcgatgc cgccagcgtg cagctggatg acaaacagcg caccatcatc
     8821 atgcaatggt ttatgtatgg cggcggcgtg ctggggcttg gcttgctgct cggtctggta
     8881 ctgccgcacc tgatcccaag ccgcaaacgc aaagatcgct ggatgaacta aatcgccttc
     8941 ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga tggcgtgaag
     9001 atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt caaagacaga
     9061 gattgggtag tggtcggcag tacgccacag gagatgctcg acgcgggcta ccagcaggta
     9121 ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc gctggcacgt
     9181 accgaacgga aatccggctc cggttacacc ggttttacct gctatgccgc accggatgtc
     9241 acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc ccaggacgat
     9301 aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc
     9361 catgtttccc ccgcttttgg cgaagatccg ttacgcgtac tgcgcgtggc gcgttttgct
     9421 gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct gatgcgcgag
     9481 atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa agagacagaa
     9541 aacgccctca ccacccgcaa tccacaggtg ttcttccagg tactgcgcga ttgcggcgca
     9601 ctgcgcgttt tattcccgga aattgacgcc ctgtttggtg ttccggcccc tgccaggtgg
     9661 catccggaaa tcgacacggg tattcatacc ttaatgacgc tctcaatggc ggcgatgctg
     9721 agtccgcagg tcgatgtccg tttcacaact ttatgccacg atctcggtaa aggactgacg
     9781 ccgccagaac tctggccgcg tcatcatggt catggcccgg cgggtgttaa gttagtggaa
     9841 caactatgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag actggtggct
     9901 gagtttcacg atctcatcca caccttccca atgctgaacc caaaaaccat cgtcaaatta
     9961 tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc
    10021 gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg actacccgca aggccgctgg
    10081 ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagccgtcgt tgaagcggga
    10141 tttaaaggtg tggagattcg cgaggagctg acccgacggc ggattgcggc ggtagccagc
    10201 tggaaggaac aacgttgccc aaagcctgag tgagtattgg gcattttgcc ggatgcggcg
    10261 taaacgcctt atccggctac atgtcaacga cagttgtagg cctgataaga cgcgcaagcg
    10321 tcgcatcaag cgttgattgc cggacgcggc gtgaacgcct tattcggcct acgcaatcgt
    10381 ggacaacgcc aggcatccga cactattcct caattaaaag aacacgacat acaccgcagc
    10441 cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag
    10501 gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa cggcaaacat
    10561 cgggatatcg ccgcttgtca ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc
    10621 catcatcatc ggcaccgcca gcaggaacga aaactcggaa gcagcgtaac ggctcacccc
    10681 catcagcatc ccacctgaaa tggtcgcccc ggaacgggag aaacccggcc acagcgccag
    10741 acactggaaa caaccaatca taaacgcctg acgataggtc atatcatcaa gacccggcgc
    10801 acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc caacgaccag
    10861 cgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga acaacagccc
    10921 cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac gacctttgct
    10981 ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa acagacgccg
    11041 ccagaacatc actactaccg ccagaattga tcctaactgg atcacaactt caaaggtttt
    11101 cgccgtgtcg ccctcaaacc ccaacaagtg accgacaata atcatatggc ccgtgctgga
    11161 taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta tcagcagcga
    11221 gtgcatatcg ctcatcaata aacccctaaa ttataaaaat gtaacgcttg tccgaactac
    11281 tgcagtatga ccaggttata accgtttggt ttaacagctg taaaattaat tattttcttt
    11341 cagattattg ccacgctcaa tgattacgcc aacattcgcc gcccgcgcca ctgcgcctgg
    11401 cttgctgagt ttgatacgca cccacggcga gttgaagcgt gctaacagca gctctgccac
    11461 ctcttccgcc acgcgctcca ccagcgcaaa acgtgccccc tcgacgtggc tgaccaccgt
    11521 ttctgcaatg tcagcgtagc tgaggcaatc cgccacatta tcgctttttg ccgctttacg
    11581 gttatcccac gccatttcga tatcgaacac taatttctgt tcgatggtct gttcccagtc
    11641 gtaaacacca atagtggtga ttaccgaaag ttgctctata aatacaatat ccatcacgac
    11701 ctgctcgctt tttggctaac ccggatacca cttccggcga aatgtgcgta ttatccacag
    11761 attcatcgtt taacacgaat tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga
    11821 atgatcctca tcgcgtacct ctgcggctcc atttccagtg ccattctggt ttgccgcctg
    11881 tgtgggctgc ccgatccgcg aaccagcggc tccggtaatc ctggcgcaac taacgtgtta
    11941 cgtataggtg gcaaaggagc agccgtagca gtactgattt tcgacgttct gaaaggaatg
    12001 ttgcccgtct ggggcgcgta tgaattaggt gtcagcccct tctggctagg cttaattgcc
    12061 atcgccgcct gtcttggaca catctggccc gttttcttcg gatttaaagg aggaaaaggc
    12121 gttgctaccg cttttggtgc catcgcaccc attggctggg atctcaccgg agtaatggcg
    12181 ggaacctggt tactgaccgt gctattgagc ggatactcgt cgctgggagc gattgtcagt
    12241 gcactgattg ctccgtttta tgtctggtgg tttaagccac aattcacctt cccggtttcg
    12301 atgctctctt gcctgatcct gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt
    12361 caggagacaa aaatctggac gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt
    12421 ctggtggatc tacatgacct gatagccttc atcgggtttg cccagccgtt gctggcacca
    12481 tgccgccaga aattccacgc agacacgtaa tttcatgctg cgatagagcg gctcccggta
    12541 aacagcccag atattggcgc tttgtgcata ctccggcaat acttgcacca gtttgccact
    12601 ctccagaaac ggcaacacat cccactcgga acgcagcata atccctttgc cctccagcgc
    12661 ccattgcagc acaatctcgc cgctattgga ggaaagatgc ccgctaacct tcaccgattt
    12721 tttctcctga ccgttcccca actcccatat tccgtgggtc atatcgcgct ctttggtcac
    12781 cagacagtca tgacgactta attcctgtag ggattgtggc tgcggatatt tttgcagata
    12841 ctcaggtgct gcgcataata ttcttttatt tttcgttaac agatgcgcaa tataataatc
    12901 aggaatttcg tcattaatac gaatatccag atcaatatta tcctgcacta aatcaatttg
    12961 ccgatcgaac aattcaaaat acacctgtaa ctcaggataa ttgcgcataa gttcggtaat
    13021 ggctggcgca atatggctgc gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc
    13081 cgggcgcgtt ttgatttgcg tgacgtcatc gaccagtcgc tgatattggg tgaggatttc
    13141 cagcgcatgt tcgtaacaac gttggccact ttcagtcagc gccacgccgc gagcggagcg
    13201 gttcagcagc gttgttgcca gcgtattttc aaggatttgg atccgcttgg tgacaaatgc
    13261 aggcgtttgc ccaagcgtcg ccgctgcagc actaaaactc ccgctgtgga cgatttctac
    13321 cagtacctgc aagtctttgg ctaaaggcca gctattcagc atcgttaatt atccgcagtt
    13381 gtgataagcg cagtgtattt cgcaaaacat tgccattccc tgctctggcg caatattcat
    13441 tcttattttc gcgttgattc acgaactgtt aattacatat tcacggtggc aaaaaatata
    13501 aaaccacatt tttagtggta gtttgtggcg gtgaattttt ccagacaaat acaaaaactg
    13561 gagttgccat gatgagcgaa agtaataagc aacaggcagt gaataagttg acggagattg
    13621 tcgctaactt taccgccatg atttctaccc gaatgcctga tgacgtggtg gataaactaa
    13681 aacagctaaa ggatgccgaa acgtcgtcga tggggaaaat tatctaccat acgatgttcg
    13741 acaacatgca aaaagcgatt gacctgaatc gtcctgcctg tcaggacacc ggggagatta
    13801 tgttcttcgt taaagtcggt tcccgcttcc cactgcttgg cgagctgcaa agcatactca
    13861 aacaagccgt ggaagaggcg accatcaaag cgccgctgcg tcacaatgcg gtagaaattt
    13921 ttgacgaagt aaacaccggc aaaaataccg gtagcggcgt accgtgggtc acctgggata
    13981 tcgtccccga cggtgacgat gcggaaatcg aagtttacat ggcaggcggc ggctgcacgc
    14041 taccaggccg ctcgaaagtg ttaatgccgt cagaaggcta cgaaggcgtg gtgaaattcg
    14101 tcttcgaaaa tatctccacc ctcgcagtaa acgcctgtcc accggtactg gtgggcgtgg
    14161 gcatcgccac ctcggtggaa accgccgccg tactctcgcg taaagccatt ttgcgcccga
    14221 ttggcagccg ccaccccaat ccaaaagcgg cagagctgga gctacgcctg gaagaaggac
    14281 tcaaccgtct ggggattggt ccacaagggc taactggcaa cagttcagtg atgggcgtgc
    14341 atatcgaatc tgccgcccgc catccgtcaa ccatcggcgt tgctgtttct accggttgct
    14401 gggcgcatcg tcgcggcaca ctgctggttc atgccgatct cacctttgaa aatctgtctc
    14461 acacccggag cgcgttatga aaaagatcct gacaaccccg atcaaagctg aagatctgca
    14521 agatattcac gtcggcgatg tgatctatct gaccggtacg ctggtgacct gccgtgacgt
    14581 ttgtcaccgc cgattgatcg aactgaaacg tccgctccct tacgatctca acggcaaagc
    14641 gattttccac gctggtccca tcgtgcgcaa aaacggcgac aaatgggaga tggtctccgt
    14701 cggtccgaca accagtatgc gtatggaaag ttttgaacgt gaatttatcg agcagaccgg
    14761 cgtgaaactg gtggtcggca aaggcggaat ggggccgctg accgaagaag gctgtcagaa
    14821 attcaaggcg ctacatgtaa ttttcccggc aggctgcgcg gtgctggcgg caacccaggt
    14881 ggaagagatt gaagaagtgc actggacaga actcgggatg ccggagtcac tgtgggtctg
    14941 ccgggtcaaa gagttcggcc cgctgattgt ctctattgat acccacggca acaacctgat
    15001 agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc atcgtggaag agatctgcga
    15061 gcacgtccat tacatcaaat aaccctcccg gagaggctcg ccccctctcc tttttcgcag
    15121 gcataacacg atgaaacctt ccactgaatg gtggcgatac cttgcgccgc tggcggtcat
    15181 cgccattatt gctctaattc cggttcccgc agggctggag agtcatacct ggctctactt
    15241 tgccgttttt actggcgtaa tcgttggact gatcctcgaa cccgtgccgg gtgccgtggt
    15301 ggcgatggtg ggtatctcca tcatcgccat actctctccc tggctgctgt tcagcccgga
    15361 gcagctcgct cagccaggct ttaaattcac tgcaaaatcc ctctcgtggg ccgtttccgg
    15421 tttttctaat tcggttatct ggctgatttt cgccgccttt atgtttggca caggctatga
    15481 aaaaaccggg ctggggcgac gtatcgcgct gattctggtg aaaaagatgg ggcatcgcac
    15541 gctgtttctt ggctatgcgg tgatgttctc cgagctgatc ctggcacctg tcacaccgtc
    15601 caactcggcg cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc cgccgctcta
    15661 tcaatcacaa ccaaacgaca gcagttcgcg cagtattggc tcgtacatca tgtggatggg
    15721 gattgttgcc gactgcgtga ccagcgccat tttcctgacg gcgatggctc ctaacttgct
    15781 gttaattgga ctgatgaaaa gcgcatctca cgccacgctg agttggggcg actggttcct
    15841 cgggatgttg ccgctcagta ttttactggt cctgctggtt ccctggctgg cttacgtgct
    15901 gtacccgcct gtactgaagt caggcgatca ggtgccgcgc tgggcagaga cggaactgca
    15961 ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg taggcgcgct
    16021 ggtgctgtgg attttcggcg gtgattatat cgatgccgcg atggtcggtt acagcgtggt
    16081 ggcgctgatg ctgcttctgc gcattatcag ttgggacgat attgtcagta ataaagcggc
    16141 gtggaacgtt ttcttctggc tggcctcgct tatcaccctc gcgaccggac tcaacaacac
    16201 cggttttatt agctggtttg gcaaactgtt agcaggcagc ttaagcggtt attcgccaac
    16261 gatggtgatg gtggcgttga ttgtggtgtt ttatctactg cgctactttt ttgccagcgc
    16321 cacggcgtat acctccgctc tcgcaccgat gatgattgcc gctgcgctgg cgatgccgga
    16381 aatcccgctg ccggtgttct gcctgatggt tggtgcggca attggtctgg ggagcattct
    16441 tacaccatac gccaccggcc ccagtccgat ttactacggc agtggttatc tgccaacggc
    16501 ggattactgg cgactggggg cgatttttgg gctgatattc ctcgtattgc tggtgattac
    16561 cggcttactg tggatgcccg tggtgttgct ttaagtaagg acggtgccgg aagtaactct
    16621 ccggcacccg atagttttac gcagccggta actccgccag cggccagcgc ggacgcacgc
    16681 taacgccgag atccgccgtc gcgcctgctt taaaccgcac cattccggca taggcgatca
    16741 tcgcgccgtt atcagtacaa aattccggac gcgcgtagaa cacttcgccg cggcgttttt
    16801 tcatcatttc agccagcttc gcccgtaacg tgcggttagc actcacgccg cccgccatga
    16861 ccagtcgctt aaagcccgtc tgatccagcg cacgcttaca cttaatcatc aacgtatcga
    16921 ccaccgcatc ttcaaaggcg cgggcgatgt cagcacgcgt ctggtcgtcg gtgccgttgt
    16981 cacgaatggt atttgccgcg aaggttttca ggccggagaa gctgaaatcc agccccggac
    17041 ggtcggtcat cggacgcggg aaaacaaagc gcccggcagt accctgcgcc gccattttcg
    17101 acagtaacgg tccgccagga taatccagcc ccagcagctt cgcggtttta tcaaacgctt
    17161 caccggcggc atcatcgata gactcgccga gcagctcgta ctgaccaatg ccagtcacgc
    17221 tgattaactg cgtatgaccg ccggaaacca gcagcgcaac aaacggaaat tccggcgggt
    17281 tatcttccag catcggcgct aacagatgcc cttccatatg gtgtacaggg atcgccggaa
    17341 cgttccaggc aaacgccaga gaacgcccca cggtcgcgcc aaccagcagt gcgccgacta
    17401 atccagggcc tgcggtatag gccacagcat caatgtcttt tgccgttaaa ccagactcct
    17461 ttagcgccgc ctggatcaac ggtacggttt tacgcacatg atcgcgggag gccagttcag
    17521 gcacaacgcc cccgtagtca gcgtgcaatt tcacctgact atacaattgg ttggctaaca
    17581 aacctttttc atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt tcaataccca
    17641 gtacacgcat gacttgtttt acctcgcttt attaccgcgc agtgtaggac caatgcgggt
    17701 tgatgtaaaa ctttgttcgc ccctggagaa agcctcgtgt atactcctca cccttataaa
    17761 agtccctttc aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca gtaaaattcc
    17821 gcaccatttt gaaataagct ggcgttgatg ccagcggcaa accgaattaa tcaaaggtga
    17881 gaggcacatg ccggtaatta aagtacgtga aaacgagccg ttcgacgtag ctctgcgtcg
    17941 cttcaagcgt tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc gtgagttcta
    18001 tgaaaaaccg actaccgaac gtaagcgcgc taaagcttct gcagtgaaac gtcacgcgaa
    18061 gaaactggct cgcgaaaacg cacgccgcac tcgtctgtac taattccccg agagcgttgc
    18121 tctccgatca gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat gcgcggctta
    18181 ttttcgttta tgaattgcta aaaatcgggg cctatggctg gacgaatccc acgcgtattc
    18241 attaatgatc tgctggcacg cactgacatc gtcgatctga tcgatgcccg tgtgaagctg
    18301 aaaaagcagg gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa aaccccgtcc
    18361 ttcaccgtta acggtgagaa acagttttac cactgctttg gatgtggcgc gcacggcaac
    18421 gcgatcgact tcctgatgaa ctacgacaag cttgagttcg tcgaaacggt cgaagagctg
    18481 gcggcaatgc acaatcttga agtgccattt gaagcaggta gcggccccag ccagatcgag
    18541 cgccatcagc ggcaaacgct ttatcagttg atggacggtc tgaatacgtt ttaccaacaa
    18601 tctttacaac aacctgttgc cacgtctgcg cgccagtatc tggaaaaacg cggattaagc
    18661 cacgaggtta tcgctcgctt tgcgattggt tttgcgcctc ccggctggga caacgtcctg
    18721 aagcggtttg gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg gatgttggtc
    18781 actaacgatc agggacgcag ttacgatcgt ttccgcgagc gggtgatgtt ccccattcgc
    18841 gataaacgcg gtcgggtgat tggttttggc gggcgtgtgc tgggcaacga tacccccaaa
    18901 tacctgaact cgccggaaac agacattttc cataaaggcc gccagcttta cggtctttat
    18961 gaagcgcagc aggataacgc tgaacccaat cgtctgcttg tggtcgaagg ctatatggac
    19021 gtggtggcgc tggcgcaata cggcattaat tacgccgttg cgtcgttagg tacgtcaacc
    19081 accgccgatc acatacaact gttgttccgc gcgaccaaca atgtcatttg ctgttatgac
    19141 ggcgaccgtg caggccgtga tgccgcatgg cgagcgctgg aaacggcgct gccttacatg
    19201 acagacggcc gtcagctacg ctttatgttt ttgcctgatg gcgaagaccc tgacacgctg
    19261 gtacgaaaag aaggtaaaga agcgtttgaa gcgcggatgg agcaggcgat gccgctttcc
    19321 gcatttctgt ttaacagcct gatgccgcaa gttgatctga gtacccctga cgggcgcgca
    19381 cgtttgagta cgctggcact gccattgata tcgcaagtgc cgggcgaaac gctgcgaata
    19441 tatcttcgtc aggaattagg caacaaatta ggcatacttg atgacagcca gcttgaacga
    19501 ttaatgccaa aagcggcaga gagcggcgtt tctcgccctg ttccgcagct aaaacgcacg
    19561 accatgcgta tacttatagg gttgctggtg caaaatccag aattagcgac gttggtcccg
    19621 ccgcttgaga atctggatga aaataagctc cctggacttg gcttattcag agaactggtc
    19681 aacacttgtc tctcccagcc aggtctgacc accgggcaac ttttagagca ctatcgtggt
    19741 acaaataatg ctgccaccct tgaaaaactg tcgatgtggg acgatatagc agataagaat
    19801 attgctgagc aaaccttcac cgactcactc aaccatatgt ttgattcgct gcttgaactg
    19861 cgccaggaag agttaatcgc tcgtgagcgc acgcatggtt taagcaacga agaacgcctg
    19921 gagctctgga cattaaacca ggagctggcg aaaaagtgat ttaacggctt aagtgccgaa
    19981 gagcgatcgg gaagcccccg acagccgcac tgagaggcag cggcaaatat ataagtacgc
    20041 cctcgtaatt atcgttggcg gtaaacaacc gttggatttc agcgttaacg gctgaaggac
    20101 atcgggtcaa tcgcccaaca ccaacctcat gaaataagtg tggataccgt cttatggagc
    20161 aaaacccgca gtcacagctg aaacttcttg tcacccgtgg taaggagcaa ggctatctga
    20221 cctatgccga ggtcaatgac catctgccgg aagatatcgt cgattcagat cagatcgaag
    20281 acatcatcca aatgatcaac gacatgggca ttcaggtgat ggaagaagca ccggatgccg
    20341 atgatctgat gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc gccgcgcagg
    20401 tgctttccag cgtggaatct gaaatcgggc gcacgactga cccggtacgc atgtacatgc
    20461 gtgaaatggg caccgttgaa ctgttgaccc gcgaaggcga aattgacatc gctaagcgta
    20521 ttgaagacgg gatcaaccag gttcaatgct ccgttgctga atatccggaa gcgatcacct
    20581 atctgctgga acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc gatctgatca
    20641 ccggctttgt tgacccgaac gcagaagaag atctggcacc taccgccact cacgtcggtt
    20701 ctgagctttc ccaggaagat ctggacgatg acgaagatga agacgaagaa gatggcgatg
    20761 acgacagcgc cgatgatgac aacagcatcg acccggaact ggctcgcgaa aaatttgcgg
    20821 aactgcgcgc tcagtacgtt gtaacgcgtg acaccatcaa agcgaaaggt cgcagtcacg
    20881 ctgccgctca ggaagagatc ctgaaactgt ctgaagtatt caaacagttc cgcctggtgc
    20941 cgaagcagtt tgactacctg gtcaacagta tgcgcgtcat gatggaccgc gttcgtacgc
    21001 aagaacgtct gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag aaaaacttca
    21061 ttactctgtt taccggcaac gaaaccagcg atacctggtt caacgcggca attgcgatga
    21121 acaagccgtg gtcggaaaaa ctgcacgatg tctctgaaga agtgcatcgc gccctgcaga
    21181 aactgcagca gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa gatatcaacc
    21241 gtcgtatgtc catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg gttgaagcga
    21301 acttacgtct ggttatttct atcgccaaga aatacaccaa ccgtggcttg cagttccttg
    21361 acctgattca ggaaggcaac atcggtctga tgaaagcggt tgataaattc gaataccgcc
    21421 gtggttacaa gttctccacc tacgcaacct ggtggatccg tcaggcgatc acccgctcta
    21481 tcgcggatca ggcgcgcacc atccgtattc cggtgcatat gattgagact atcaacaaac
    21541 tcaaccgtat ttctcgccag atgctgcaag agatgggccg tgaaccgacg ccggaagaac
    21601 tggctgaacg tatgctgatg ccggaagaca agatccgcaa agtgctgaag atcgccaaag
    21661 agccaatctc catggaaacg ccgatcggtg atgatgaaga ttcgcatctg ggggatttca
    21721 tcgaggatac caccctcgag ctgccgctgg attctgcgac caccgaaagc ctgcgtgcgg
    21781 caacacacga cgtgctggct ggcctgaccg cgcgtgaagc gaaagttctg cgtatgcgtt
    21841 tcggtatcga tatgaacacc gatcacacgc tggaagaagt gggtaaacag ttcgacgtta
    21901 cccgtgaacg tatccgtcag atcgaagcga aggcgctgcg caaactgcgt cacccgagcc
    21961 gttctgaagt gctgcgtagc ttcctggacg attaatcggt aggccggatc aggcgttacg
    22021 ccgcacccgg cacctgtccc tttgcacaaa cgccaccttt tcggtggcgt tttttatcgc
    22081 ccacgcacca ccagcgcctg gtccagctcg cgatacgctt caaccagttt ctccagtgaa
    22141 acgcgactta aaccgctggg atttggcagc acccaaatct gcgtcgaacc aatgctgagc
    22201 gtttgtttcc cccactgtgc accacgctgg ctgaatccct gttcatatgc ttgtttgccc
    22261 agaatcgcca acgcttgcgg ctgataatct tcaatttttt caatcagctt acgcccacct
    22321 gcgtgtagct cttgctttga aatttcattg gcttgcaccg ttggcctgtc taccagtttg
    22381 gtgacgccac aacgataatc cagcagatgc tgtgcctcct gcggcttcaa ctgatggtcg
    22441 gtaaacccgg cctgatatat caccttccag aagcgatttg ccggatgagc aaagggaaaa
    22501 ccagtcccgg cggatgaaag cccagggttg ataccgcaaa acacgacccg taaccctgga
    22561 gccaaaatat cctcaaccat atttactcct ttctaacatc accaggaaaa gtataacgga
    22621 ttgaaaatac attgtttata aaaacagcag gcgcgcggta atggctggat tgcgacacgg
    22681 agttacttta taatccgcta ccatggcccc ttagctcagt ggttagagca ggcgactcat
    22741 aatcgcttgg tcgctggttc aagtccagca ggggccacca gatatagcaa aggctgacga
    22801 gaaatcgtca gcctttttct ttttatatat cagttacttt gcgtgccagt aggccgctgc
    22861 acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg cggcttaaat tcttaacgac
    22921 tttaccttcg ccggttatcc agatgaagta atcatcggca gggatttgca tctgcgccag
    22981 acgcgcatct accgcctgct catcatgtgc cagccattcg atattaaaac catcaaggtg
    23041 cgcgagataa tcctgacagg cgttatcccg cacgctaacc agcgcactaa cttgcggttt
    23101 aacggcaagt ttgctcaacg tttccaggcg gcggcgcaat gcgggcattc cggattcatc
    23161 gcagacatac agctgatacg cgtaatcttc cggcaccacc agcgaaccgc gcggacctgc
    23221 caccgtaagt ttatcgcccg gttgcgcctg catcgcccag ccgctggcga ccccaccgtc
    23281 gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg tcatacagcg gcgtatagtc
    23341 acgcgacggt gggcgtggtc cttccggcca gacgatgccc tcttccgtta ccgttggcgg
    23401 cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga
    23461 tgtaaaaccg tccagcgcct cgccgccaag gacaatgcgc tgaaaaccgg cgctgatacg
    23521 ctcaacgcgt agcacagtca attcacggaa gcgcagatca ttgcgaacgc gctgcgggta
    23581 gcggggggag ttattcattg ttatcgcctt cgtgatggta atcagatata tctaaataat
    23641 tttcgcaaat gataatgatt gttaatcatg ataaa
//
LOCUS       DBFIFS010000052        23259 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.052, whole genome shotgun sequence.
ACCESSION   DBFIFS010000052 DBFIFS010000000
VERSION     DBFIFS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.052"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            122..6436
                     /locus_tag="N7A07_001906"
     CDS             122..6436
                     /locus_tag="N7A07_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2476580.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEEPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPATA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            6660..9518
                     /locus_tag="N7A07_001907"
     CDS             6660..9518
                     /locus_tag="N7A07_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2476581.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            9521..14455
                     /locus_tag="N7A07_001908"
     CDS             9521..14455
                     /locus_tag="N7A07_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476582.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            14455..20796
                     /locus_tag="N7A07_001909"
     CDS             14455..20796
                     /locus_tag="N7A07_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2476583.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            20793..22907
                     /locus_tag="N7A07_001910"
     CDS             20793..22907
                     /locus_tag="N7A07_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGU2476584.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         5892 a         5543 c         6241 g         5583 t
ORIGIN      
        1 aaaaagataa taacctgata catcaactgt ctgcgctttt tgaatttcac tatcagcaag
       61 ccccacagca attaggggta atctatcaat attattttga acaagcagga gtatgtcatt
      121 gatggacgtt cagcaactgc attatgcgcc acttctaaat cagcttcatc agcgtgcggt
      181 gaggtcggtg gtaagtcagt tggcattgcg gagcaagtcg ttgactcatt atctggaatc
      241 acaatatttt cagcctcccg gtcaggaggg gtcactgctt gcagacccgg tattcgaaag
      301 tacattcggc tggatgcctg cggatgaaac aatggaggat ttgcgggaca agctgatctc
      361 cagcccgctg gttgatgcat tgagcaaagc gccagtgcca tttcgtcatc aacttactgc
      421 gtggcgaacg attctggaag acaaaaaatc cttgttagtg agcagcggta cgggatccgg
      481 gaaaacagaa tgctttatgg taccggttct ggaagatctt attcgtcagc aaaaatcgac
      541 ccgccagtca ctaacaggga cacaagctat tttcttgtat ccgttaaatg cattaattaa
      601 cagccaacag gagcggcttc gcgaatggac gcgcggattt aaggggaaaa tacgcttcgc
      661 gctatataat ggcgaaaccc ggcataccaa atacgaaatt caggaagatc agttgaaagt
      721 acctgaacaa gctttgagtc gggaagccat ttatgaagaa ccaccttcca tcatggtgac
      781 gaataccacc atgctggaat atatgctgat tcgtcgtaaa gatgccccaa tcatcgaaaa
      841 gtctcagggg atgttaaagt atatcgtcct ggatgaagcc catagttata ttggttctca
      901 ggctgctgag ttagcgctgc ttttacgtcg tgttatgcag gctttcaatg ttggacccgg
      961 cacagataaa cctgtgcaga ttatcgcgac ctctgccact attggtgaag actctcctga
     1021 aggtaataag gagcttgcta aatttgtcgc tgatctcgcg ggcgttacag aacgtgatgt
     1081 caaagtggtt cgcggttatc gacaaatacc gcgggtatcg gagtcgctga ttcgtcatga
     1141 ataccccaca tctttaactt ctcttaaatc cctgagccct caggatcttt accaacagct
     1201 ttgtcattac cgtgtggccc agcaattgcg tcaggcgtta actcatcccg gacggcaggc
     1261 agtacgtttg agtgagttgc tcaatgttgc cagaagaacc tggccagata ttaatcaccg
     1321 tgagctactg caactcctcg atctaatggc aagatcgcga gaaggtgaac tggcttttac
     1381 gccattacgt atgcatgggt ttatcagaac actggcgggt ctatgggcat gttcaaacaa
     1441 acaatgctca cataaagcac atgaattaaa tcagagtgac tggccatttg gtcaggtatg
     1501 gtttgagcag cgccaatatt gtgactgtgg agcgccggta tttgaagttt tacgttgctc
     1561 cgggtgtggt tcagcttatt tgtctgccaa agaggagatg cgcggtgatg gaacgaactg
     1621 gttggtagcg caaccagcca ctgccgaagt ggatgagttt gctcttgatg tagatgttta
     1681 ttctgaagat gaagacaacg acgaattaga caataactct caatttgatc gtctgattgc
     1741 gagcgatggt gaaaaatata tcatttcact ggaagaaacg acggcgggga aaattgatag
     1801 tgaagcgcaa aagcactatg aaataaacct gttaagaccg gaatctcgag gtgagggacg
     1861 caataactcc tttgcttgtg tttgctgtgg tgacactcaa cggaaaaata atcctttatt
     1921 ccgtccgctt cggctcggcg cgcccttttt tttaaatgaa attattccta ctctgctgga
     1981 gttttctcca ttaccgcaaa caagagaaga gcagttagga ccgtttaatg gtcgacgctt
     2041 gctcaccttt acggattcgc gtcagggtac cgctcgcatt tcagcccgct tacagcagga
     2101 tgccgataaa gcgacgttgc gagctctttg ttaccaggag ctggcaaata ttacagagcc
     2161 ttcagcaaaa ctcagcccgg cacactgtgc actgttagag aaagttatta gccagttttc
     2221 tgtcctccct ttaaaaaatt ccatcgacgc gctgaaaaaa gcgatggctg cgaatgagga
     2281 gcttagcccg acggacattc aggcacttaa aattattgag ccgatggtga gttccttaag
     2341 cacggagcat gctgaagcga tgcaaatact tcaggctaac tctggcaatc agcagaagaa
     2401 aaccatgtca tggcaaacgc tgtctgaaca gctggttggc agtgtggacc ttgccgatag
     2461 aatgagaggc agttttaaag aattgtctgg gcttgatctt actaaacaac agtttgcaga
     2521 tttttgttta tacagcgagt ttggtcgccg ccctaaaaat gcctggaccc tcgaaagtct
     2581 gggtctggta gccattcact atccattcat tgataaagtt acaacctgcc cgcaggactg
     2641 gaagacatta ttacccgatc ccgataagca attagcggaa tggcgtaatt tattgaagat
     2701 tactcttgat ttctttatcc gtgaaaacag tgccgttttt tacgaaaacg agcaataccc
     2761 tcgctggatg ggggcacgtt tcccggtaaa aatgctccag gggccagata aaaaaaacaa
     2821 agggcagaaa cgtgatcaac tttggccaca aatacgcgat cgccattata accgggtcat
     2881 taagctatta atcgcagtct ttccggcaat acaacctgag caggctcact ggaaatcgtt
     2941 agttaaccac ttaatgattg aggtctggga tgcaattcga ccctgcttac gtcagtttga
     3001 gtcgggttat cagctggata ttaaacaaca agctgaattt tacagtccaa agcaagtgtg
     3061 gcgttgcccc tatactcgac gtgcgttaga cgtcacgttg ctgggatact ctccgtattt
     3121 gccgggtagc aaagaaattg cacctgaaaa agcagtgttg attgaaatgc ctgagcttcc
     3181 tgtacgtcac tggcgtttgt ctggtggtgg agaaatcgcc cgagaagaac gtctggagtg
     3241 gctggaaagt aatgcgctga ttcaacatgc ccgcgaggaa ggattatggt ctactcgcag
     3301 cgaccgactg gccctgaaag atagctggta tcgtctggag gaacactctg cgcagagaac
     3361 gcctgagcag aaccagttta atgaaaaaca atttaagtca ggcaaagtta atgtgctgaa
     3421 ctgttccacg accatggaga tgggggtgga tattggtggt atgagtttgg tggcaatgaa
     3481 caacgttcct ccagcgccgg caaattattt gcaacgtgca ggtcgcgcgg ggcgtcgtgg
     3541 agagagtgca tctgctgcca ttacgttatg taaaaacacg gcgcatggaa tggaggtatt
     3601 caaagacccc ctctgggcgt tcaacactac tgccagtgcg ccacgggttc gttttgggag
     3661 cagtagtatt gttcaacgac atgttaatgc cctggtttta ggtctgtttt tacgagctga
     3721 agttcctgat gcgacaaaac tgagttgcaa atggttcttt gaaggtgatg agagccagtg
     3781 tcttcgtttt ctccattggt taaatcacca ggccgatcag ctggcggata aacttaaacg
     3841 ccttactcag ggaaccgtac tgatgtcatt gacggcaacc cagctcttga cccgcacaca
     3901 agcaatgatg cagcaggttg atatccgctg gagaagccaa cttgcgatac tgttggaaaa
     3961 tatcgaagcg ttgaaggcgg ataacagcgc gtgggaagaa acaccagcag gtaaagccat
     4021 cgcttatcag cttcgtgatt atcgcggtgc atatctgttt tctaaactca ttagtgaagc
     4081 gttcctgccg gggcatggtt tcccggttgg cgtagtcaat ttcaattatc tgacggcaga
     4141 tgagctggaa aaacgtcgcg caataaaagc cactcaggct gaccctaatg aagggggaga
     4201 aagcttttct cgtcgtattg aaaaattgcc cagccgtgat ttgcctacag cattacgcga
     4261 gtatgcgcct ggtgcagatg tggtattggg cggaaaagta tatcgttcct cgggcattat
     4321 gcttggcaag gtgcttgcca gcgggcagga gctgtcaggc gatcatcata ttccgtggtt
     4381 ctggcattgc cgtaaatgtg gtgcgggggc aaccagtact acccatcctg tcgaatgtag
     4441 tcactgcaaa gcggatatac agcaacttga tgtgaagcgt tatttacagc ctgttggttt
     4501 tgcgacagat attcgttatc aggcacataa tgatgtatcc atgcctgcac aacttccctg
     4561 gaaagatcca cgtgttcttg taccgtcttc ggtgtgggtt tctttacccg atgctgggct
     4621 aggacgttac cgttttagcc atagtgggga attgttccac ttcagtgaag gggaatttgg
     4681 gcacggctac gcaatttgcc tctcatgtgg tcgggcagaa tctcaaactc aaccccagcg
     4741 tacccctgaa aatctaaaga acccagagag ggaaaatacg cattacctgt tgcgaggggg
     4801 cagtaacgat cgtcagggga gcaataaatt gtgccacggt catgtgcata aagatctgtg
     4861 gttgggatat agcagtcgta cagatatggt tgagctgcaa ctcaatgatg ataatggcct
     4921 gttgatccgt gatgaggttg ccgcgcgttc ccttgcggtc gctttgcgtg aagggttagc
     4981 gcataaattg ggtatcgaaa atactgagct gggcgtgacg acgcagcaag cgcgcgatat
     5041 caatggctat accggatatt ccatcttcat ttacgacaat aatgcgggtg gtgcaggtta
     5101 tgcagtgcaa ttgatcgacc attgggctga tgtctttaat tacgccagaa aattgctcga
     5161 ttgcagttgc gataaattct gtcaccactg cttgctcagc tacgacagtc agcattatgt
     5221 caacagatta gatcgtcacc atgcattaac cctgcttacc aatgtacggt tgcagcgact
     5281 taacctcgcg ccagaatatc agtacttcgg cgatggtagc cgggttgaaa ctaaccctct
     5341 gagtttacgt attgcgcaat gcctgaacag cgagatttat gactcctgct cgctggttct
     5401 tgcggggccg caggaacagt gggactttgc ccaatggcca ttatttaaag agttgctcca
     5461 gtttgcctcc tccggtggga atgttgagtt gctggtggcc acgccgctgg caaacttaac
     5521 tgatagctcc agacatcagc tttccgcgtt ggcggcgatg ccgggaggtc gcttacaagt
     5581 gaaatccatt gccactgcgc agctcatgca aggaaagggg cgctggcttg cgcaggtgac
     5641 tcgcgaaggg caaagccaac agtgggccgc agacgacagc gctaccgttg cacctggtga
     5701 attatgggga caaagtgcgt cttcaccagt agtgacactt aaaggaacgt ccggaaaaac
     5761 cttctcgggt cagaccttaa gtgcagaaga tttactgccc gccttaccca ccggggcggt
     5821 gcgcatcaat ctctgtgaac aactggacgg gccgctggaa ggttttggtt cgcgtttctg
     5881 gtcgttagtc acgcaacagc acgctggctg gaagcaggca tttacccgcc acaaagagat
     5941 aacgcatgtt gagtacagcg atcgttatct taatagccca ttcacggcgc gtttgttagg
     6001 tgaaatctta accgagctgg ttgaacaggg aatggcggaa cgcgccagcc tcactgtttg
     6061 cgtcaaaaaa ttggattaca actctcgcca acacgacgct ctctataacg cgtggcttaa
     6121 tgaggaagat cgtcagcagg ttgtgaccac tttacttgaa gaaggatatc tggggcctgc
     6181 gtggccggga gcaattagct ggttaacggg ggataaccag agtacggagc atggacgaga
     6241 actgacagtc accttcagcg atggcagtca gcattatgtc ctgctcgaca tggggcttag
     6301 ctactggcgc tgtattgaag ataccttctt tgattttgcc ctgcgagtac cgcagcaagt
     6361 tgaacggctg gcaaacacgc gagcgcgagc tgttgccccg ggtaatgacc ttaggagtta
     6421 tattatcgct ggataaataa ttgctcccct ggggatatct tccaggggag ttgacccagc
     6481 tcacttttta ataatttttg tttgaaaatc atgcgcaatt tatgcgcatt ccctcccatt
     6541 ttgtcacttt gccgggggcc ttcatgatat ctttaaggcc ctctttttta tcgcagtgtg
     6601 ccgcgccaca tttttcagga tgacgttacc gccactgccc caaatagaca gaaaaaccga
     6661 tgaataagca aaattatgca cctggtatgc gggtggttat tcgtgatgcc gaatggcgta
     6721 ttcgccgggc ggatgacagt ggtgatggtg ggtatttgct gacctgcgat ggtatttcag
     6781 agcttgtgcg cggtaaagaa gggttatttc tgaccaaact ggaacaaaaa gtagagatcc
     6841 tggatcccgc aaaaacgcat ttggtggaag atgaatccgc caattatcaa gcggcacagt
     6901 tgtatattga aagtcagtta cgccaacgcg taccgacaga cagtaaagtc cattttggtc
     6961 atctggcggc gatggattcc atgccgtttc agctcgatcc tacgcggatg gcgctggcac
     7021 aaccgcgtca gcggatactc attgccgacg ctgttggtct gggtaaaacg ctggaagccg
     7081 gtattctggt ttcggaactg atccgtcgtg ggcgcggaaa acgtattctg gttctggcgg
     7141 ttaaatcgat gctgacacaa ttccagaagg agttctggag ccgttttgcg atcccattga
     7201 cccgcctgga ttctgctggt ttacaaaagg tgcgtaaccg catcccaacc aaccacaacc
     7261 cgttccacta ttttgacaag accattatct ccatcgatac gctgaaacaa gacattgaat
     7321 atcgtcacca tctggaaaat gcctggtggg atattattgt cattgatgag gcccataacg
     7381 tcgcagaacg tggaaccagt tcgttacgca gtaaactggc aaaactgctc gccgggcgca
     7441 gcgatacgct gattatgctt tcagcaacac cgcatgatgg taaggctgaa agttttgcca
     7501 gcctgatgaa catgcttgac ccgacggcga tcgccaaccc gaaagagtat gaatacgccg
     7561 attttgccga taaaaatctg gttgtccgtc gcttcaaaaa agacgtgaaa gatcagatgt
     7621 ccggcgagtt ccccgagcgc aatattgtca aattgactcg cctggcatcc ggtgccgaag
     7681 aagaagcata ccgtcgtctg gttgagagcc agttccgtga tgatgacgat gaacaggccc
     7741 agtctaacaa aggtcgtctg ttcaagataa ccctcgaaaa agcgctattt tccagcccga
     7801 tggcgtgtgc cagcgtcgtg gcgaatcgtc tgaagcgtct tgagagccgt aaagatcata
     7861 atagccagag ccagatcaac gaactggagt cattgctgtt ggcgctgaat aacattgatg
     7921 ccagccagtt tagcaaatac cagttgctgc tcgacaccat ccgaaaagac ctcgcctgga
     7981 aagccaataa cacggaagat cgcctggtga tcttcaccga aagtattaaa acgctggagt
     8041 tcctcgaaca gcaactgcga gcggatctga aattgaaaga tgaccagatc gccacgctgc
     8101 gcggcgatca gggcgatacg gtattgatgg aaaccgtaga agcctttggt aaaacgcagt
     8161 cgccgttgcg tcttctggtt tgttcggatg tggcatctga agggatcaac ctgcatcacc
     8221 ttagccacaa aatgattcac tttgatattc cgtggtcgtt gatggtattc cagcaacgta
     8281 acgggcgtat tgaccgctat gggcaaaaac atcagcccca aatccgctat ctgcttaccg
     8341 aggccagcga accacagatc aatggcgata tgcgcgtact ggaagtgttg atcaacaaag
     8401 acgaacaggc gcagaagaac attggggaca gttcggagtt taccggcaaa tttacccagg
     8461 aagaggaaga agagcaggtt gcagagttca tgatgcagga tgatggtgcc agcctgtttg
     8521 atcaactgct gaacagcaac gtctcggaaa gcgccgaaca cgatctgttt ggcgaaatat
     8581 gcagtgcggt ttccagcgat gcctcaatgg tcaccgaaac agacaccagc ttgtttgcca
     8641 gcgaacaggc gtactgcgaa agagcattgg gttacctgaa agccagtggt caaactatcc
     8701 agtatgaaac cttgcctgat aataccttgt cgctggtggc accggaggag ttacgccgcc
     8761 gcttcaacca gctaccgcct gaaattgccc cggagaactg gcagctctat ttaagtcagg
     8821 ataaaaccgt catcaccgac gcgattgcgc gcgctcgcgg tgagcaacat gcctggcccg
     8881 atgtgcagta tctctggcaa atcaacccgg tagtgcagtg gctggacgat aaaatctctt
     8941 cggcttttgg tcgtcatcag gctccggtta tccggctgcc atacctgctt gaacccgatg
     9001 aagatcactt cattctttcc gggttattcc cgaaccgtaa atcacatccg atggtgaacc
     9061 cgtggatagt ggtgagcttt aaccgtgaat cgctaatcgg cagtcagcct ttcgctgagt
     9121 ttttacaacg tcatccgcag ttgagtaaca agctgactaa cagcggcggt aaagatcgta
     9181 atcaccaacg ccagcaggat ttactggaag cggctattgc tcatgcccgg gaggtattca
     9241 tccatgatcg gaatgcgttt gaaacgcaca ttaaccagca actgaatgag catctgcaaa
     9301 agctggacgt tttgcgtggg cggcagttga gccaacttga gctggatttt gccgataaca
     9361 aacagcaatt gtcagtcaag cagagccgta aagagcagag acaacgcgaa atcgaacaca
     9421 attttgacag ctatatcgaa tggattgagg acaccatgac gactgaaaaa gaaccctaca
     9481 ttcaggtaat tgctgttatc accggagcgg agggttaatc atggcgctgg tcggtattaa
     9541 taacgaaaac gaattttact ctaaccacta tttgggtgag gttttcacca gtgatatccg
     9601 cgatgtgctg gaaccctgga tagcccagga aaatgcagcg cgtgaagcgg agcgtgccgc
     9661 tcgtgaacag ggcaaagacg tggagccggg ataccgcgct ccgtggaacc agtttaacag
     9721 tctggcgact gagtttttcc gcaaacttgc cgagcacgaa aaacagcgtc agatcccgca
     9781 gcgtctggcc gatcaacgta atcgctggca gccattgtta aaggcgctgg gctacgaaat
     9841 tacgccacag atccaaatgc tggatgacga tacaccgctg ccggtactgg cgcgttacaa
     9901 cagcactgac ggtagcccgt ggctgtggat tgttgaagca cacgatcagg aagaaggaac
     9961 gctggatccg ctggcgctct ccttactgac cgcgcaattc ccggcggata ccgacaaaca
    10021 taagcacgac agcctgcgca aaaaagccaa cggtgaatat cgcagctggc aggatttgct
    10081 ctctacggcg gtcttcaccc aaaatgaacc gccgcgtttt gtgctgctgc tcggtaaccg
    10141 tcagctattg ttgttggacc gtactaagtg ggcgcaaaac cgtctgctac gttttgattt
    10201 tgaagagatt ttaagccgtc gtgaaacgga tacgctgaaa gcgactgcgg tgttgttaca
    10261 taaagattct ctgctgccgg gcagtggggc accttatctt gactcgctgg atgacaattc
    10321 gcacaaacat gcgtttggtg tttcggaaga tctgaaatat gccctgcgcg aaagcattga
    10381 gttgctgggc aacgaagcga tgcattatct gatcgaccgt ggcctggcaa actatacagg
    10441 taatcgtgcg gtggacccgg atgaactgag ccgcgaatgt ctgcgttaca tgtaccgcct
    10501 gctgttcctg ttctacattg aagcgcgccc ggagctgggt tatgcgccaa tgaccgccaa
    10561 aacctatctg caaggttaca gcctggaaac gttgcgcgat ctggagatga tcccgctgac
    10621 cagcgaagaa gatcgcaacg ggcgctactt ccacgacagc ctgaatatgc tgtttaaact
    10681 ggtgcgcgaa ggctacaacg gcggcgtgaa aatgcagagt gacctggaga gcggcgaccg
    10741 gatcaccatc catagtcatc agttcagcgt cccgcgtctg gaaagtcatc tgtttgatgc
    10801 caacaacacg cgcattctta accgcgtggt attccgtaac gaaaccctgc aacagattat
    10861 ccaggcgatg tcgttaagcc gcccggccaa agggcgcttt aaccgccgcg gacgtatttc
    10921 ttatcgccag ttgggtatca accagttggg tgcggtgtat gaggcgctgc tctcctatcg
    10981 cggattcttc gccagcgaag atctctacga ggtgaagaaa gccggggaag agtttaacga
    11041 gctggagacg ggttacttcg tcagtaagga tgagattagc aaataccacg aagacgagaa
    11101 ggtctacgag aaagacggca gtctgcgcat tcaccgcaaa ggcagcttta tctaccgtat
    11161 ggccgggcgc gaccgtgaga aatctgcctc ttattacacc ccggaagtgc tgacccgctc
    11221 actggttaaa tatgccctga aagaactgtt taaggagcag attgatccca ttagcgatcc
    11281 gcacgccaaa gctgatgcca tcttaaacct caccgtgtgc gaaccggcga tgggcagcgc
    11341 ggcgttcctt aacgaagcca tcaaccagct ggcggaagcg tatctgttcc acaagcagca
    11401 ggcggaaggt cgccgtattc cgcaggatcg ttacacccag gagttacagc gggtgaaaat
    11461 gtacattgcc gacaacaacg ttttcggcgt ggacttaaac ccggtggcgg tggaactggc
    11521 ggaagtgtcg ctgtggctga acgccattag tggcgatgcg tttgtaccgt ggtttggtta
    11581 ccagctgcac tgcggtaact cgctggtggg cgcgcgccgt caggtgttca acaagagcga
    11641 actgacctac aaaaaagcca aagatccgag ctggcttaac agcgagccgg tcgaactggc
    11701 gatgaacacg ccgcgtgaag agacgcagat tttccacttc ctgctgcccg acggcggtat
    11761 ggctaactac agcgataaaa ctgttaagca gcgttatccg gatgacttca aagcgctgga
    11821 cagctggcgc aaagagttta ttaaaagctt tgccgggcat gagattgctg atgtgcagcg
    11881 tatcagcgaa aaggtggaag cactgtggaa cacctatcgc cagcaactta aagcagaacg
    11941 tctgaaaacc gccgacagct acccggtgtg gccggcagaa aacagcgagc agacgcgttc
    12001 ttcgctgagc agtaaagatg aaacctttag cggtcgtctt gaagataaca gcgcctacca
    12061 gaagctgcgt tgggtaatgg actactggtg cgcgctgtgg ttctggccga tcgacaaagc
    12121 cgatgagcta ccggatcgcg gcacctggtt gtttgagatt gaaaccctgc ttgacgggat
    12181 tgtaatcacg gaaaaagtca ctgaagttgc ggagcacacc accggcgatc tgtttgccga
    12241 agaaggcctg ctgcgagaag agtcttcgct gttttctgtt gctggtcgtc tgaaaaccga
    12301 ggtgttgttc cgtcatttgc cgcgtctggc gattgtcgat gccctgagaa agcagcaccg
    12361 tttcttccac tgggatctgg agttctgcga cctgtttgcc gagcgcggcg gttttgacct
    12421 gatgctcgga aacccgccgt ggctgaaagt ggaatggcag gaagcgggcg tgctgggtga
    12481 ttacgagccg gaatttgtgc tgcgtaagct gagcgcctcg aagctggcaa cgttgcgtat
    12541 tgataccttt aaccagatcc cggcgctgga agcggcctgg cgcagcgagt atgaaggctg
    12601 tgaagggatg caaaacttcc tgaatgcgca gcagaactac ccggtactgc gcggggtgca
    12661 gaccaacttg tataaatgct ttctgccgca ggcctggcga ttaggggcgc agaaaggcgt
    12721 ggcaggtttc ctgcacccgg aagggattta tgatgacccg aaaggcgggc aattacgtgc
    12781 ggcggtatat ccgaggctga gggcgcattt tcagtttcag aatgagttaa atttgtttgt
    12841 tgaagttgat caccatgcga agtttagcag caatatttat tctgctagcc ctagcacagt
    12901 gggatttgaa catatatcta atttgtatgc tccgcaaact attgatgcat gttttgaaca
    12961 ttctggcagt ggggacattc ccggtctcaa agacgagatt gagagcgagg gaaaattaaa
    13021 agttgtatgg aacacatctg gccaccgttc tcgattaata agtatcgcca ctcatgagct
    13081 agaattattt gctcgtctat atgacagcga aggaacgcca gcctggcagg cacgtttgcc
    13141 agccttacat gctaaacaac ttgttgctgt actggaaaag tttgctaatc agccgaatag
    13201 attaggtgat ttgcaggggc agtatttttc aacggttatg ttcgatgaaa catatgctca
    13261 gagggatggg acaattttac ggcagactca attccctcaa gattcatcac aatgggtact
    13321 gtctggccct catttctttg ttgggacgcc gttctacaag actccgcgcg aaaactgtac
    13381 gcttaacagc gattatgact gcctggactt gctaactctg cctgacgact atctgccgcg
    13441 cactaactac attccggcat gtgatgcaca ggagtatgca aaacgtactc catgcgttac
    13501 atggactgaa ctggctgaag atgaaccgaa gaaggtaaca gattattatc gcttagctat
    13561 cagagccatg ttggctcaat cgggggaacg tacactaatt agtgctattt atccgccaga
    13621 aataagtcac atgaacgcag tacgttctta ctgctatagc tcacagaatc tgttactcga
    13681 acattcaggt atgtgttttt ctttaccttt tgattttatt tgtaaatcta ctggcaaggc
    13741 aaacttacat cagatgcttg atggtttctc atacgtatta ttcaatccga gacaaaaggc
    13801 attattatac tgcttagtat tatcattaaa ttctgtaaat gatgtatatg ctggcctttg
    13861 gcaatcctgc tacaccccag acttcaacac ccagcgttgg agccgcgatc tcccgcagct
    13921 cccccaggat ttcttcgcca aactgacccc agagtggcag cgtaactgcg ctttacgctc
    13981 tgactacagt cgtcgtcagg cgctggtgga aatcgacgta ttggtggcgc aggcgctggg
    14041 gttaactctc gaagagctgc ttaccattta tcgcgttcag ttcccggtga tgcgccagta
    14101 cgaagcggat acctggtacg atcaaaacgg tcgcattatc tttaccccaa gcaaagggct
    14161 ggtgggcgtt ggcttgcctc gcaccgcgcg taaagctgac ctgaaaaacg gctttgtctt
    14221 taacgtcgac agcccggagt ggaccggcgg tgactgcacc gatcaagcta tcggttggga
    14281 tgatgtcaaa catcttaaaa ccggtaccgt cagcgtcacc tttgatgatt atacccgcag
    14341 cgacgaaggt gagcgccgta ccgtcacctg gcaggctccg tttatcaagc cagatcgcga
    14401 agatgactac aaagtggcct gggcgttctt tgcacaagat aaggagagcg cctgatgttg
    14461 ccgtccgttg tcagtcggca ggtcgccgac agcgttgccg cctttttacg ggcggcgttc
    14521 ccgctaaaca gcccgctgtt taacggtgag aataacgata atgtctcaat gctggagcaa
    14581 tttctggccc agccggaaac gctgctgaaa ggaccatacc tttccgccca gttgccgttt
    14641 cgtaaaagtg acttaccgct gaattttttc cctaatctga cgctgccgtt cccgccgcac
    14701 gcgcatcagg cccgggcatt tcagcgtctg ggaagcgacg cgccacagcc gacgctggtc
    14761 gcaaccggta ccggctccgg taaaacagaa tgttttatgt tcccgctgct caaccactgc
    14821 gcgggcgcgt cacaagccgg ggttaaagcg attatcatct acccgatgaa cgccctggca
    14881 accgatcagg ccagccgttt cgccaaaacc atcgccagcg atccacaact gcatggcaag
    14941 gtgaccgtcg gcctgtttgt cggcgacagc gaaatcgaac ccagcaaaaa aatgtcggca
    15001 gaaaaggtca ttacctgtaa gcacacgctg cgcgaaagcc cgccggacat cctgttgacc
    15061 aactacaaaa tgctcgacta cctgctgatg cgccctggcg atcagccgct ctggcgttac
    15121 aaccagcccg gttcattgcg ttacctggtc gtggatgaat tgcatacgtt tgatggcgcg
    15181 caagggtcgg atctggcctg tctggtgcga cgtttgaagc atcacatagg tgtggataac
    15241 caacgattcg cctgcgtcgg gacatcagct accgtcggcg acgaactggg gcaactgctc
    15301 gattacgcaa aaacaatctt tgaccagccg tttaccgatg acgccgttat tcgcgaagat
    15361 cgctacacgg cagcggagta cttgcagaat tacgccattg cgtacagtca gtatccgggg
    15421 ccggaggtcg cttcggccct ggacccgcag agttacgcta ccccggtagc gtatcttaac
    15481 gggcaaatcc ctttgtggtt ccccgacagc gacttgcaac tcccggacga tctggagagc
    15541 gacgacgggc gtgaaaagcg tattgcgctg gggtcactgt tacgccgcca cagcgtgatg
    15601 catgtcttac tccatgatct gcaaggcggg atcctctctg aacgagagtg tctggaaaat
    15661 ctccaggtca tgctggcaga aagcgccgac catgcccgac gcgtgctgca aagcattctg
    15721 gcactgatcg cgcaggcacg tctcgaagta ccggaaacgg aacacgatcg gcaaaaacgt
    15781 ctccaggcga agaaagcgcg tccggtgctg ccgtttgtgc aactgcgctc ccagttgtgg
    15841 cttcgtgaga tgcgtcgtct ggtcgccagc gtggcgaaaa cgccccgact ggtgtttgcc
    15901 gatgatgtgg cggtggaaga tcgcgaacac tatttgccgg ttatccactg ccgtgactgc
    15961 cacgcaactg gctgggccag cctgcgtcat gggcaaagcg ttcagttgga aacagacctc
    16021 gacggcattt atcgccagtt ctttgaaaaa gggcgctcca tcgtccttgc tttcccggat
    16081 aacaacgaaa aagcggtcag tggcgcacac cagaaactgt gtcctacctg tctgaaactg
    16141 aaccgacagt ccagcgtcca gtgtggacat tgcggtcata cggaactgtt gcaggtactg
    16201 atcccggata tgctcaaaga gcgtaaggac gagctggagt ttgctaatga atgcccgtac
    16261 tgcaacagca aacaggggat ttcgattctg gggtcacggg ctgccagtct gtcggcggtg
    16321 atgattcacc agctttatgg cagccagttc aacgaacata aaaagctgat tacgttttct
    16381 gactcggtac aagacgccgc gcatcgtgcc agcttcttta ctgcccgtac ctggccgctg
    16441 atgattcgtg ggcaaatcgc cagtgttctt agcgaaaaag agacattacc gctggatgcg
    16501 ttcgcggcgc gtgtttgcca acaaacgcgt gaacgttcac cggacgatgc tcattttgca
    16561 gcaaacgtca tcgcaccaaa tatgcagtgg ctgaatgatt atcagacgct gaaaaatgaa
    16621 ggcaaactgg cggagaactc cgatctgccg gagctggttt ccctgcgcct cgactgggag
    16681 gtgtacagcg aatttaccct gcgcgcgcgt atcggacgta cgctggaagc caccggttat
    16741 gccgttgccg gagtggataa agatcgcttc aatccggcaa ttaccgccct gcatcaacag
    16801 ctttgtgaag agctgggtga tgagatgtct ggtgtgagcg tcgagcaggt agcgcacctg
    16861 gcgctggggg ttatctggta tcaacgccag gccggggccg ttatgcatcc ggcgttcgaa
    16921 agttatcgcc gtgatggcac gacctttatg atgacgcaaa aagagcgtac ctcgcgctat
    16981 atgccttcta tcggtccgaa aacgcgtaaa ccggcttatg cgtcgtttga aaaaattaat
    17041 ggtttccatc gcctgattgg cgcgaagtct aaccccagtt ggtatcagca ttggatcaat
    17101 cgcacgctga gtaacggcaa taatctgttt atcagtagcg ttgcggaaac agtgttacga
    17161 cgcctgttta cggcgttgaa aatagccgga gtagtaaaag attttgacac caaagggcgt
    17221 gaagcctggg gactggtgcc ttcggcgctg gtggtgagcc gtgacgtcgt tcagcttaat
    17281 tgctcatgtt gccgtgaggt tgtccgggct ccggccgatc aaggctggca gtggcgtaat
    17341 gcgccttgtc tctctttacg ttgtgaaggt cgttaccagg aagtcgacaa tacggcgacc
    17401 tggcgttggg agaacatgga catcgcgcgc gtacagggcg cagaacacac ggggctgctt
    17461 agccgtgaag atcgcgaagt caccgaaaaa tcgttctacc gcggcaacca accgtggaat
    17521 atcaacctgt tgtcggcaac accgacgctg gagatgggta ttgacgttgg ggatctttcc
    17581 accgttttac tctgttctgt cccacctgct caggccaact acttgcagcg tattggtcgc
    17641 gccggtcgta aagatggtaa cgcgttaaac attaccgtgg cagaagggaa cccgcacgat
    17701 cagtttttct ttgaagaacc actggagatg atgcagggcc aggtgcaggc tcctggcgtc
    17761 ttccttaatg caacggctat tctcgaacgc cagctggcag ctttttgtat ggataactgg
    17821 gtgaaaaccg gtgtaccaga atccgctatc tgtaaaaacg tcaaacagat gctggatgag
    17881 ctggagttcg ggcgtaaatc gggttttccg tataacttac tgcgatatat cgaacagcat
    17941 catgcggaga ttgctgcgca atttacggca atcttccccg atcttgccgc agaaactcgc
    18001 caacagctgt tgtcttatct tcagggcgcg ccaggacaac gctcgctggt gcagcgtatc
    18061 gaagaagcgt taaaactgct ggtggaagat cgcaaatctt tgcgctcacg catcgataaa
    18121 ctcaagcgca gtatcgataa actggagaac gcgccgcgcg atcaaaactt tgatagcgat
    18181 atgcgcgagt taacttcgga acggcaggcg ctgatggcgc tggtcaatca aatcaacaat
    18241 aagcaaacgc tgaatttcct cacggatgaa ggtctgctgc ctaactacgc cttcccggaa
    18301 gccggtatta cgttacgttc tgtgctctgg cggcgtaaag aaggcgggga agcacgtgaa
    18361 tatcaaaaca ccactttcga atatgaacgc ccggcatcaa cggcgctggc tgagctggct
    18421 ccgctgaata atttctatgc cggtgggcat aaagttgaaa ttgagcagat agacctgaag
    18481 gtctctgagc cggagaactg gcgtatctgt agccactgta actacagcga aaatatcgac
    18541 cagacgggcg accagcataa atattgcccg aaatgtggca caccggggtg ggccgatgcc
    18601 ggacaaaaaa ccacgctgct gaaacttcgc caggtttatg cccgcgccag cgcacgtgat
    18661 agccagatca gcgacgagag cgacagccgt gaacctgctt tcttccagcg tcagctattg
    18721 gttagtttcg aaaaagaaga tgtatcggcg gcatacgcga ttgaggaagg cgaagttccg
    18781 tttggttttg aatttttaag caaagtcacc ctgcgggata tcaacttcgg caaaatggcg
    18841 gatgatgcca atgagttgat gattgccgga gaagcgaaga agcgaaccgg gtttaaagtt
    18901 tgcctcgggt gcggcatggt gcagcgtcct cgcgatcatg aaccacgtca tgatttgagc
    18961 tgcaaatatc gcacggaacc ggaaaaggcg aagttcgaag attatctcta cctctatcgc
    19021 cagctggagt cagaagcgtt gcgtattctg ctcccggtaa gcagttacag taatgaccgt
    19081 gtcgtggagg cttctcttgg ggcggcgatc cagctcggtc ttaaacatta cttcaagggg
    19141 aatgtcgatc acctgaaagg cgttgtgtac cgcgagccgg aaaatgaagg cgaatcctgg
    19201 cggcagtatc tggtgattta cgataccgtc ccggggggga ctggttctct taaagaactg
    19261 atgcgcactc ccgacaattt gctgaaatta cttgaactgg cctatcaggc aatagtggaa
    19321 tgcagttgca acaatgatac gcataaagat ggttgttatc gttgcgttta cgcttaccgt
    19381 gaccgtgggc gcatgaagta tgtctcccgc gatcaggcgc gtctcttgct ggcgaaaatt
    19441 ttgcaggcaa gagattcgat acgggtaatt gattccatca aaaacatttc cctcgaagcc
    19501 atgatgggca gtgagctgga aaaacggttt attagctgcc tgcaagaaaa taaaaacctg
    19561 attgtcagtc gcagttacgc gcatcagggc gctgggtgga ttattaacac ccgcagcgaa
    19621 ccgatgatga gctggcatct taaagctcag gtggatttag gcgctaaaga ggcggtgggg
    19681 atcccttgtc gcccggatta cgtgctatat ccgcttatgc agtctgatgc cattaaaccg
    19741 gtggctattt tccttgatgg ctttgcgttc cacaaaaata gcgtcgcaga ggatgtgcaa
    19801 aaacgccagg cgattcggga tagcggtaat ttctgggtgt ggacagtcac gtgggccgat
    19861 ctcaaagagc cagggcttaa acatgttcag gatgtcctgg gacttgggca taacccggat
    19921 atgaaacagc ctaagtttta taacctgttt catgatacca actttgcggc ccttgaagcg
    19981 tcgttccggg aacgcaacag ttttgcactg ctgctggatt atctcgccga tccgggcgga
    20041 agaacgcagt tatggcagag aatggctgct gcacatgcct gggtgtggtt ggatgtgaag
    20101 aagtcacagg acgcgggaac gaaacaaaag tatgcctatg aaatgcagga aaacgcgcca
    20161 gcttggcggc tagcagagct tttgccagac gaaccgtttg tctttggtgg tttactcgat
    20221 agctgtaact catcgcagca atttattgag ctggcttcgg tgttaccaca acaagccatt
    20281 aaaccgacga ccagcgtcgc acaaatgcgc agctggctgc gtttgcatat ctgttttgac
    20341 gatcgctata cgcaggatga cggttatgaa gcgggactca atggcttctg gcgactggta
    20401 aacctgttac agttcctgcc ggatatgacc tttaccagcc gtaaagccgt gcatttaccg
    20461 caggaaagcg ttgacactaa agtttcggca acgccagagg cagtaccaga gactgatgag
    20521 agttgggctg agattattga gtttggctta ttaagcgctg aagagatcgc gttgcttcag
    20581 tctcattctc tctctgcgcc gactctcggc tacgaattgc aaaatgatga aggtgaaatt
    20641 attgcggaag ccgatctcgc ctggccatca caaaaatggg cattgattat tgataatcaa
    20701 gaatttattc cattattcac aaccaggggt tggcacgttg cattcgggcc aattgacgaa
    20761 aatacattgc aacatctgtc cggaggtgac aaatgacggt gcaaacgccc aaggtggcac
    20821 tttctgatgg ttttctgggc gcattcgcgc ggatcccaaa ggcacagcaa aaaaaggtgc
    20881 aggaattcat ctccaaattc cgtcaggacc cgaccagtaa tggacttaac tatgaaaaga
    20941 tccacgatgc gcgcagtaaa aatgtccatt cggtgcgaat cgatcaaact tatcgcggca
    21001 ttgttctgaa acctgaacag ggtgccctgt atatgctgat gtgggtggat aaacacgacg
    21061 aagcatatga ctgggcgcgg cggcatgatt gttcaattca cccggttacc ggcgcgattc
    21121 aggtcattga tattagctat atcaaaccgg cagcggaaac agtggttgat aaaccgaaac
    21181 tcttcgctgc ttacagcgcg gaacagattc tggctctggg tgtgccgcct gtctttattg
    21241 atcaggtgat ggcgcttacc gatgaagccg ggcttaatca acttgaaagc ataatgccag
    21301 cagaagcctg ggaacctctg cactggctgg cagaagggct ggattaccag gaagtgctgg
    21361 aagagtttaa cgatcatcgt gatgagcctg ttgataccaa cgactttatg gaggcgattg
    21421 aacgaagtaa acgtcgcttc catgtcgttg aaaatgaaca ggagctgttg cagatgctta
    21481 atgcgccttt agaaaaatgg cgtgtatttc tgcatccgag ccagcgaaaa ttggtggagt
    21541 cgcccgctaa tggtccggta cgagtactgg gcggtgcagg tacagggaaa acggttgttg
    21601 cgatgcaccg tgcgcggtgg ttatctcagc gtctggcaga taaacccggt aagaaagtac
    21661 ttttcacgac ctttacgcgt aacctggcgg cagatattcg cgctaatctc cagcggttgt
    21721 gtacgcgaga agagatggcg cgtattgacg tcgttaacat tgatgcctgg atgagcgacc
    21781 aactaaaacg ccacggttat gatttccgcg tggtatacga cagcgatgaa gggcgacgca
    21841 aatgctggaa ctatgccttg cagcaggcac ctgccgaact ggggctacct gataatttct
    21901 atgctgaaga gtggcaacgc gttgtccagc caaatgcggt ttacacccgg gaggaatacc
    21961 tcaaagtcag ccgggttgga cgtggtacag cgttatcccg tattcaacgg gcgaaaatct
    22021 ggccagtctt tgaagaattc agagcacaaa tggcgcgtgc caaactgcgc gaaatgggcg
    22081 atgccatgca tgaggctatt gttctgttta aagaaaagca ggtccaacta ccttatagca
    22141 gtattgtggt cgatgaagct caagatatcg gtgccccggc atttactctt attcgcagcc
    22201 tggttcccga gtcgcccaac gatctgttta ttgtcgggga tgggcatcag cgtatttatc
    22261 gcaataaggt cgttcttggt cagtgtggta ttaacgtccg tggtcgtcgc agtaaaaaat
    22321 tgaagattaa ctatcggacc acagaagaga cgcgtcaatt tgctgttggg ctgttaactg
    22381 gtgtgaaggt tgataacctc gatggcgaag cggataccag taatgattat ttgtcattac
    22441 tgcacgggga aaagcccatg attacgcatg cggcagattt caaagaagag gctgcaacgc
    22501 tcgtcaagca aattcaggca ttgctggcga atcaggttcg ttctcaggat atctgcatta
    22561 ctgcgcgtac taaacatgct tgtgaccgct acgcatcagc actgaatgac gccggaattg
    22621 agacatttaa tttaggcaat gatagcggtg acagcgatgc acgccctggc gtgcgggttg
    22681 caacgatgca ccgtattaaa gggcttgagt tccagtatgt gttcctggtg gggattaacg
    22741 aaggcgttgt gccggaaata aaagcattag catccgatga tcccgttgaa cagcgtgatg
    22801 cattatttaa tgagcgcgcg ttactgcatg tggctgcaac ccgtgccgta aaagggctgt
    22861 ttgtttcatc atctggtgtc cctagtccgc tattagtggc tgactgataa tatgtccaaa
    22921 acaggccagg agccgaaacg ctcctggtct atataaccgt tctggcaact ggcgatattt
    22981 atattgctta gctcccaaca gccagacaat atcaacttag aaattatatt cgcttatgcg
    23041 gtttgcatat tgcacagtac aacctgtttt ctctatatct aacgtaacgt caggatgtgg
    23101 atgatttttg gatggggtga cacactggac aaatatagct ggcacgaaaa ggctggcatt
    23161 tccagctgac caatagtctc tggaaccatg atgatgataa aataaatatc ttaagtttta
    23221 aacgtatgat gccaggatta ccagaattat aaacgaata
//
LOCUS       DBFIFS010000053        22583 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.053, whole genome shotgun sequence.
ACCESSION   DBFIFS010000053 DBFIFS010000000
VERSION     DBFIFS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.053"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(218..1129)
                     /gene="gsiD"
                     /locus_tag="N7A07_001911"
     CDS             complement(218..1129)
                     /gene="gsiD"
                     /locus_tag="N7A07_001911"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU2476585.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1132..2052)
                     /gene="gsiC"
                     /locus_tag="N7A07_001912"
     CDS             complement(1132..2052)
                     /gene="gsiC"
                     /locus_tag="N7A07_001912"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU2476586.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2070..3608)
                     /gene="gsiB"
                     /locus_tag="N7A07_001913"
     CDS             complement(2070..3608)
                     /gene="gsiB"
                     /locus_tag="N7A07_001913"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU2476587.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3628..5499)
                     /gene="gsiA"
                     /locus_tag="N7A07_001914"
     CDS             complement(3628..5499)
                     /gene="gsiA"
                     /locus_tag="N7A07_001914"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU2476588.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5486..6451)
                     /gene="iaaA"
                     /locus_tag="N7A07_001915"
                     /pseudo
     CDS             complement(5486..6451)
                     /gene="iaaA"
                     /locus_tag="N7A07_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            6655..7890
                     /gene="moeA"
                     /locus_tag="N7A07_001916"
     CDS             6655..7890
                     /gene="moeA"
                     /locus_tag="N7A07_001916"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU2476589.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7890..8639
                     /gene="moeB"
                     /locus_tag="N7A07_001917"
     CDS             7890..8639
                     /gene="moeB"
                     /locus_tag="N7A07_001917"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU2476590.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENVLTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8818..9480)
                     /gene="fsa"
                     /locus_tag="N7A07_001918"
     CDS             complement(8818..9480)
                     /gene="fsa"
                     /locus_tag="N7A07_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU2476591.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9611..10510
                     /gene="ybiY"
                     /locus_tag="N7A07_001919"
     CDS             9611..10510
                     /gene="ybiY"
                     /locus_tag="N7A07_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU2476592.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10516..12947
                     /gene="ybiW"
                     /locus_tag="N7A07_001920"
                     /pseudo
     CDS             10516..12947
                     /gene="ybiW"
                     /locus_tag="N7A07_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            13093..13908
                     /gene="ybiV"
                     /locus_tag="N7A07_001921"
     CDS             13093..13908
                     /gene="ybiV"
                     /locus_tag="N7A07_001921"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU2476593.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14060..15325
                     /gene="ybiU"
                     /locus_tag="N7A07_001922"
     CDS             14060..15325
                     /gene="ybiU"
                     /locus_tag="N7A07_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU2476594.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15493..17085)
                     /gene="ybiT"
                     /locus_tag="N7A07_001923"
     CDS             complement(15493..17085)
                     /gene="ybiT"
                     /locus_tag="N7A07_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU2476595.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17304..18224
                     /gene="ldtB"
                     /locus_tag="N7A07_001924"
     CDS             17304..18224
                     /gene="ldtB"
                     /locus_tag="N7A07_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2476596.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18282..19400)
                     /gene="ybiR"
                     /locus_tag="N7A07_001925"
     CDS             complement(18282..19400)
                     /gene="ybiR"
                     /locus_tag="N7A07_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU2476597.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19397..19864)
                     /gene="mntR"
                     /locus_tag="N7A07_001926"
     CDS             complement(19397..19864)
                     /gene="mntR"
                     /locus_tag="N7A07_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU2476598.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20050..20178
                     /gene="mntS"
                     /locus_tag="N7A07_001927"
     CDS             20050..20178
                     /gene="mntS"
                     /locus_tag="N7A07_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU2476599.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20450..22033
                     /gene="opgE"
                     /locus_tag="N7A07_001928"
     CDS             20450..22033
                     /gene="opgE"
                     /locus_tag="N7A07_001928"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU2476600.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22082..>22583)
                     /gene="ompX"
                     /locus_tag="N7A07_001929"
     CDS             complement(22082..>22583)
                     /gene="ompX"
                     /locus_tag="N7A07_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU2476601.1"
                     /translation="CLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKMGGFNL
                     KYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASIYGVVG
                     VGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIA
                     GVGYRF"
BASE COUNT         5528 a         6236 c         5625 g         5194 t
ORIGIN      
        1 ttatcagcct tatctttatc ttttcgtata aactcagcat gttaatacat ttaacaaaat
       61 aactgtctga acgctgtgcc gctgcaaaaa cagtcccttg ctgcggctat ttaaccaaat
      121 tacccgattc tacaaaatca gcagaaagtt acaacacttg cttagggcaa taatttcctg
      181 tgaggtgatt actctttcaa gcaatattca aacgtaacta tcctttaatt ttcggatcca
      241 gcgcatcgcg taaaccatcg cccaacaaat tgaacgccag tacagtcaga aaaatagcca
      301 gggccggaaa aacagcgaca tgcggcgcga taaccatatc cgctcgagcc tcattgagca
      361 ttgctcccca ctctggtgtc ggcggctgcg caccgaggcc gagaaatgac aggctggcgg
      421 cagagataat cgaggtacca atgcgcatgg tgaaaaacac cacgatagaa gagacggtcc
      481 caggcaggat atgacgcaac aaaatggtca tatcgctggc accaatactg cgtgctgact
      541 caataaaggt ttgctgtttc aacaccagcg tgttgccgcg caccaggcgg gcaaacgcag
      601 ggatggaaaa aatggcgact gcaataatca cgttagcaat gccgcttccc aacaccgcaa
      661 caacagcgat cgccagtaaa atacccggga aggcaaacag cacatcgcaa atgcgcatga
      721 tcagccgatc ccaccagcct tcataatatc cagccaacaa gcccagcaac gtccctatcg
      781 ccgcaccgat aaacacggca aacacgcccg ccgccagcga gatttgcgca ccaaccagga
      841 cacggctgaa aatgtcgcgc cccagtgaat cgacgccaaa ccagtgctgc aaagaaggtc
      901 cgttattcag attgtcataa tcaaaataat tttcggcgtc atagggagcg atccagcgtg
      961 caaaaatggc caccacaatc aataaaataa cgaataatgc ggcggtcatc gccatatgct
     1021 gacggcgaaa tcgtcgccag aattcatgcc acggtgtacg tacctggtca ggtttgacca
     1081 gtggcatggc gtttaacacc gcctgacgtc gccagttaaa tagtcgcatc cttacttgta
     1141 cctgatagcc gggttaatgg cggcgtaaag cacatccacc actaagttga taagaataaa
     1201 ttccagcgag aaaagcagaa tttccgcctg aatcaccggg taatcgcgca tttctacgga
     1261 gtcaacgagt aagcgtccaa gtccgggcca gttgaaaact ttctccacca cgatggaacc
     1321 accgagcaaa aagccaaact gtaagcccat catggtcact accgggatca tcgcgttgcg
     1381 tagcccgtgt ttgaggacaa cccaggtttc gctcacccct ttcgccctcg cggtacgcat
     1441 ataatcttcg cttaaaacgt cgacaaacga cgcgcgggta aagcgcgcca tcacggcggc
     1501 cactgccgcg ccgagcgtca gggagggtaa aatgtagtgc tgccaactgt ctgctcccac
     1561 ggtaggcagc cagcccagtt caacggagaa tacctgaatt aaaagcatcc ccagcgcaaa
     1621 tgccggaaac gagatccccg acaccgcaat ggtcatactc aatcgatccg gccaacggtt
     1681 acgccagacg gcggcgataa ttcccgccgc cataccaaat atcaccgccc agaccatact
     1741 ggttatggtc agccacagcg ttggcataaa gcgcctggca atctcatcgg caaccggacg
     1801 acgtgacacc atcgacaggc caaaatcccc ctgcacagca ttgctgatat agtgccagaa
     1861 ctggtgatac agcggctgat ccaaccccag ctgctgacgc accagttcta taacctgcgc
     1921 atcagcttcg ggcccggcaa tcaatcgcgc cggatcgccg ggcagcatat gcacaaataa
     1981 aaacaccagc accgagacga taaacagcgt cggaatcaac cccagtaatc gtttgataac
     2041 gtaattaagc attccactcc ctgcgttgct tattgcaaat ccgcgtcttc aaagctgaag
     2101 ccggtatctg gcatgatcca aaaaccggtc aggtttttac tgtgtgccga caccagtttt
     2161 tctaccacca gcgggatcca cggcgactct tgccagatga tatcctgcgc cgccttatat
     2221 aagcgggtct tttccgccgg atcattagtt ttcagtgcct gagccaggaa gtcatccacc
     2281 tgtttattgc tgtaaaacgc ggtattaaac agcgtcggtg gccagttttg cgaggcaaac
     2341 aacggcgata gtgcccagtc cgcttcgccg gttgaagccg accagccagt gtagaacatc
     2401 cgcacgccgc tctctttttg ccctttacct tcaacttctg ccgcccgctg tccggcatcc
     2461 atcgcagtca cctgggcttt aatcccgacc tgcgctaact gctgctgggt aaattgcagc
     2521 actttctgcg cggtgctgtg gttatgtgac gaccacagcg tggtactgaa accgttggga
     2581 tatcccgcct ctttcagtaa ttcgcgcgct ttcactggat cgtaaggcca cggtttataa
     2641 ctttgcgcgt aggcgatact cggcggtacc acaccagtag ctggcgttgc atagcccgcg
     2701 aaggccactt tcaccagcgc cggacggtta atggcgtaat tcagcgcctc acggaccttc
     2761 gggttatcga atggcttttg cgtcacgttc atactgatat aacgctgcat aattgacgga
     2821 ctggccatca actcgatatt tttgtttttc tccagcagtg cggcctgctc gtaaggaatg
     2881 gggaaagcaa actgcgcttc accggtttgc agcattgccg cgcgggtgtt gttatccgcc
     2941 accggacgcc aggttatgct gtccagtttg ggcaatcctg gctgccagta acccgcgaat
     3001 tttttcacct tcacaaaatc ggtctgattc caggtatcca gttcatacgg tccggttccc
     3061 accggatgaa aaccaatctc cttgccatat ttttccagcg ctgccggtga aatcatcgcg
     3121 gtcgccggat gggcaagaat attaataaac gctgagaacg gctgtttgag ggtaatcttt
     3181 accgttgtcg gatcgatcgc ttccgtttta gcaatattct tatacaggtt atagcgttta
     3241 agatgattcg ccggatcgct ggcccggtcc agattcgctt ttaccgccgc tgcgttgaaa
     3301 tcggtgccat cctggaattt aattccttcc cgcaatttca cggtgtaagt aatgccgtca
     3361 tcggaaacgg tataactctc cgccagcacg tttttcagtt tcatctcttt atccagaccg
     3421 aacagcccct ggtaaaacga tttcgctacg gcctgagata acgtgtcatt cgcgtcatac
     3481 ggatcgagcg tggtgaaatt cgatcctacc gccaccacca catctttggc agcgaatgca
     3541 caagatgcca tcaacgctgt cgcaatgccc agcgccacta acccactacg gtgtacagct
     3601 cttgccattt tattctccgc ctgaatgtta tctacgcatg aatgcgtatt ctgattgtgg
     3661 ttgtgcgacg taatgccccg gcccgacgca ttgcaacgag acggctgcca cctcttcgcc
     3721 acgcagatga atattgctgg gaagatcgtc cgacagcagt acacgctgcg gtcgttgtcg
     3781 ggacggttca gcgaccggaa ctgccgccag taatttacgc gtataaggat gctgcgggtt
     3841 ttcgaagacc gcgcgccgtg gaccaatttc aacaatttgc ccgagataca tcaccgccac
     3901 acgatgacta atccgctcta ccacagccat atcgtgggag ataaacagat acgcaatgcc
     3961 gaaatcacgc tggagatcga gcaacaagtt gataatctgc ccgcgaattg aaacatccag
     4021 cgccgaaacg gcttcgtcgg caatgatcac ttttggattc aatgccaacg cgcgagcaat
     4081 gcagatgcgc tggcgctgac cgccggaaaa ctcatgcggg taacgccagg catgttcagg
     4141 taacaggccc acgcgctcca gcaaccacgc aacgcgtgca accgcttctt tacctggcaa
     4201 taaaccgtgt acgcgcagcg gttcgataat cgaatcaccg atggtctgac gtgggtccag
     4261 cgaagcgtaa gggtcctgaa aaataaactg aatatcgcgg cgcaatgcct gaagtttgcc
     4321 gggtgacaag gtatcgattc gctgaccgtt aaagataatt tcgccgccct gcgattcgac
     4381 caggcgcagc aacgcccgcc cggtagtgga tttaccgctg ccagactcgc ccaccagcga
     4441 tagcgtttcg ccaggccaga gatcaaaact gactttctca acggcatgca cttcccgcgt
     4501 tacgcgattc aacaaaccgc tgcgcaaagg gaaacgggtg accagattac gcacccgtaa
     4561 aacaggttcg ccatccacca ccgttttctg ctcgatgggg ggggcctgtt tcgctggatg
     4621 ttcaagcgat atcaatggga aacgtcgggg ataatctaac cctttcatcg caccaagttg
     4681 cggaacagca gctaacagcg cacgggtgta gggatgttgc ggtgcatgaa aaatctgttc
     4741 gacggtaccc gtttccaccg cctcgccctg atacatcacc agtacccgat cggcaatctc
     4801 tgccaccacg cccatatcgt gagtgataaa gataacgccc atcgacatct ctttttgcaa
     4861 tactttgatt aattgcagga tctgcgcctg aatagtgaca tccagcgcgg tggttggctc
     4921 atcggctatc agcactgccg ggcggcatga cagcgccatc gcaatcatca ctcgctggcg
     4981 catcccgcca gagagttgat gcggataacg tgaaagaatg gtttgtgcct ccggaatgcg
     5041 tacctgatcc agcatccgct tcgcctcgac catcgcttct tcacgactgg cgttctgatg
     5101 cagacgaatt gattcggcaa tctgttcacc cacagtaaat actgggttca gcgatgtcat
     5161 cggctcctga aatatcatcg ccatatccgc accgcgcaca tggcgcattt gtgcagcgct
     5221 ctgctcgcta agttcaatca catcacgact gcgccgccgc aacagcattt tatcgcactg
     5281 cactaaaccg cccgcctgtt ccaacagacg catcaatgcc agcgcagtca ctgacttacc
     5341 ggagccggat tcgccaacaa ttgccagcgt ctcaccgcgt tgcagactaa aagagagatt
     5401 gcggaccgca gctattttct gctggtcctg cataaaggca atattaagat tttcaaccgc
     5461 cagcacatta ccggcatcaa gttcatcact gtgtggcaac ggtgtcccct ttttcacggt
     5521 agataccggt ggttggcgta tcgcctgcgt agccccaggc gcgatacatt ccttcggtgt
     5581 taaacggtag cgcgacattc ccttcatggt cgatagcgat taagccaccg ctaccgccaa
     5641 gcgcagggag tttttccatt actacccgct cgcaggcttc cgcgagactt aatccgccgt
     5701 aatccattaa cgcggcgatg tcatatgccg ccagcgcgcg gatgaagact tcgcccgtgc
     5761 cggtacaaga aaccgccaca ctggcgttat tggcgtagca tccggcaccc actaaggggc
     5821 tatcgccaac tcgtccgggt aatttattgg tcattccgcc cgtggacgtg gctgccgcca
     5881 gattgccgtc taaatccaac gccacggccc ccacggtgcc cattttctgt ttttcatcca
     5941 gtggcgcacc gctatggtcg aggactgttg ccccttcctc gcgcgctgcc attagttgtt
     6001 cataacgcaa aggcgtggag aaaatctccg gtgagacgcg ctccatgcca tgagcaaacg
     6061 caaaattttc tgccccttcg ccaatcatca tcacatgcgg gctttactcc atcaccagcc
     6121 gggcggcaag aaccggatta cgcagatgac taacgcccgc caccgcaccg gctttcaggg
     6181 tgttaccatc catcacacag gcgtccagtt catgcgtttc atcacgcgta aagacggcgc
     6241 caattccggc gttaaacagt ggacactctt ccagcagacg caccgcttcc gtcaccacat
     6301 ccagcgcact ttcgcccgct tccagcattt tctgcccggt ttcaacaatg gcagacaacg
     6361 cctcgatgta gcgtaattcc tgttgcagac tcatctgcgc gcggctaatt gcacctgcgc
     6421 caccatgaat tgcaatgact gctttgccca tagtattcgt ccattaaggg tggctcgcca
     6481 cttttgctat aaatatcaga atttttatga attactcatt caatttttga atatagaaag
     6541 atggatatgg catgtaaagg caggacccag cgctacgccc gaggtggcat acagtctgtg
     6601 gcgtttttct gacataatag gcaaattcga ttttgcctcc gcaggagtgt tttcatggaa
     6661 tttaccaccg gattgatgtc gctcgacacc gcgcttaatg agatgctttc tcgcgtcacc
     6721 ccactgaccg cccaggaaac gctgccactg gttcagtgtt ttggtcgtat tctggcgagc
     6781 gatgtcgttt cgccgctgga tgtcccaggg tttgataact ccgcaatgga tggttacgcg
     6841 gtacgtttgg ccgatatcac ctccggacaa ccgctgcccg ttgccggtaa atcctttgcc
     6901 ggtcagccgt accatggtga atggcctgcg ggtacctgca ttcgtattat gaccggtgcg
     6961 ccggtgccgg aaggctgcga agcggtggtg atgcaggagc agactgaaca aacggacaat
     7021 ggcgtgcgtt ttactgctga agtgcgtagc gggcaaaata ttcgccgtcg cggtgaagat
     7081 atctctgcag gtgcggttgt tttcccggcg gggactcgcc tgactaccgc agagctgcca
     7141 gtaattgctt cactggggat tgccgaagtt ccggtgattc gtaaagtgcg tgtagcgctt
     7201 ttttctaccg gtgatgaact ccagttgcca ggccagccgc tgggcgacgg ccaaatctac
     7261 gataccaacc gtctcgccgt acacctgatg ttagaacagc tgggttgcga ggtaatcaac
     7321 ttagggatta tccgcgacga tccacatgcc ctgcgcgccg catttattga agccgacagc
     7381 caggcggatg tggtgatcag ttccggcggt gtttcagtgg gtgaagcgga ttacaccaaa
     7441 acgattcttg aagagctggg ggagatcgcc ttctggaagc tggcgattaa accaggtaaa
     7501 ccgttcgcgt tcggtaaact cagcaatagc tggttctgcg gcctgccggg caacccggtt
     7561 tcagcgacgc tgaccttcta tcaactggta cagcctttgc tggcaaaact aagcggcaac
     7621 accgccagcg gcctgcccgc gcgccagcgc gtacgcacag cgtcccgcct gaaaaaaacg
     7681 ccaggacgtc ttgatttcca gcgcggcgtg ctgcaccgca acgccgatgg cgaactggaa
     7741 gtgacgacca ccggacatca gggttcacat atattcagct cctttagcct cggcaactgc
     7801 tttatcgtgc tggaacgcga tcgcggcaat gtggaagtgg gcgaatgggt ggaagtagaa
     7861 ccgtttaacg cgttgttcgg aggcctgtaa tggcggaact cagcgatcaa gagatgctgc
     7921 gctacaaccg gcaaatcatt ctgcgcggtt ttgattttga cggtcaggag gcgctgaaag
     7981 attctcgtgt gctggtcgtc ggcctcggtg gactcggctg cgcagcttcg cagtatctgg
     8041 caagcgccgg tgtcggcaac ctgacgctgc tcgacttcga ctcggtttcg ctctcgaatc
     8101 tgcaacgcca gacgctgcac agtgatgcta cggtcgggca accgaaggtg gaatccgccc
     8161 gtgacgctct gacgcggatc aacccacata tcgcgattac gccagtcaat gcactgctgg
     8221 atgacgcaga acttgcagca ttgattgctg aacacaatct tgtgctcgac tgtacggata
     8281 acgttgcggt acgtaatcaa ctgaacgcag gctgttttgc cgcgaaggtg ccgctggttt
     8341 ccggcgcggc aattcgtatg gaagggcaaa tcacggtctt cacttatcag gacggcgaac
     8401 cgtgctatcg ctgccttagc cgtttgttta gcgagaacgt gttaacctgc gtagaagcag
     8461 gcgtgatggc accgttgatc ggcgtgattg gttcgttgca ggcaatggaa gcgatcaaac
     8521 tgctggcagg ttatggcaaa ccggccagcg ggaaaatcgt catgtacgat gcgatgacct
     8581 gtcagtttcg cgaaatgaaa ctgatgcgca atccggggtg tgaggtgtgc gggcagtaat
     8641 tgccacctcc aggcctgata agacgcgaca agcgtcgcat caggcatttg ctccagacgc
     8701 cagatgcggc gtgaacgcct tatctggcct acggttcggg cacagatttc caagcgcggc
     8761 aggcatcggt gcactccgcc acgtcatcag caatttatga ggatgcagaa cgtccggtta
     8821 aatcgacgtt ctgccaaacg ctccctgcca gtcctgctca aacttcgcca cagcggcatc
     8881 aaccgccgga tagctaatca tctgttgtgc cacatccagt ggcagagtaa ttgattcaca
     8941 tcctgccagt aagcagtcca gcgcctgacg cggggttttg aaactcgcgg ctaacacttt
     9001 cgcccgcggc gcatgcattt tcaataactg gtgtaagtcg gtcacagtct gaataccgct
     9061 accgccctga gcatcaatac gattaacgta aggcgcaaca tattccgcac ctgccagcgc
     9121 cgacagcagc ccttgtgctg cgccatatac cgcggttccc agcgtcggaa tcccttccgc
     9181 ttttaacatc ttaatagctg ccagcccctc ggtggtcacc ggaactttca ccacgatatc
     9241 cgcaataata gaacgcaatt tacgtgcgtc attcaccatc ccttcagcag tggtagccat
     9301 tacctgggca aacagacgcc cctgaccgcc catcgcttca tgaagttccg gaagcacaac
     9361 ctccagcggt tttttacccg cggcgataat gcttgggtta gtggtcacac ccgccagcgg
     9421 aaaaatacgt gacagcgcct tcaccgcaac aacgtctgaa gtatccagat acagttccat
     9481 aaccatcctc aaaatgtact ttttgtctga atgtagacta tcacgggaaa actctctgaa
     9541 cagttgacgc acatcaatct aactttcatt cgaaagtatt ttaatatttg aacgaaacag
     9601 agaggcaact atgattttca atattcagcg ctactcgacc catgatggcc ccggtatccg
     9661 cacggtcgta tttcttaaag gctgttcgct gggctgccgc tggtgtcaga acccggaaag
     9721 ccgcgcccgc acgcaggatc tgctgtatga cccacgtctg tgcctggaag gctgtgagct
     9781 gtgcgctaag accgcgccgg aagtgattga gcgcgcgctg aatggtttgc ttattcatcg
     9841 ggaaaagtta accccggagc atctgacggc gttaaccgac tgctgcccga cacagtcgtt
     9901 aaccgtgtgt ggggaagtga aaagcgttga ggagatcatg gtgaccgttc tgcgcgataa
     9961 accgttttac gatcgcagcg gcggcggctt aacactttct ggcggtgaac cattcatgca
    10021 gccggaaatg gcgacggcgc tactgcaagc cagccacgag gccggcattc atactgcggt
    10081 agaaacctgc ctgcatgtgc cgtggaaata tatcgcccct tctctgccct atatcgatct
    10141 gtttcttgcc gatttaaaac acgttgccga cgcgccgttt aaacagtgga ccgacggtaa
    10201 cgccgccagg gtgctggata acctgaaaaa actcgccgca gcgggcaaaa aaatcattat
    10261 ccgcgtgccg ctgattcaag gctttaatgc cgacgaaacc tctgtgaaag ccattaccga
    10321 ttttgccgcc gacgagctgc acgttggcga aattcatttc ctgccctacc acacgctggg
    10381 catcaacaaa tatcacttac ttaatctgcc ctatgacgcc ccggaaaaac cgcttgatgc
    10441 gccagaactg ctcgactttg cccagcagta tgcctgccag aaagggttaa ccgcgacctt
    10501 acgaggataa caaccatgac cacactgaaa ctggacacgc tcagcgaccg cattaaagcg
    10561 cacaaaaatg cgctggtgca tattgtgaaa ccgccggttt gtaccgagcg cgcgcagcac
    10621 tataccgaga tgtatcaaca acatctcgat aagccgatcc ctgtacgtcg cgcgctggcg
    10681 ctggcacatc acctggcgaa tcgcactatc tggatcaagc acgatgagtt gatcatcggc
    10741 aaccaggcaa gcgaagttcg cgccgcgccg atcttcccgg aatatactgt ctcgtggatc
    10801 gaaaaagaga ttgatgatct ggcagatcgt cccggtgctg gctttgcggt gagcgaagag
    10861 aacaaacgcg ttctgcatga agtgtgcccg tggtggcgcg gtcagacggt acaggatcgc
    10921 tgctacggca tgtttaccga tgagcaaaaa ggtctgctgg cgaccggaat cattaaagcg
    10981 gaaggcaata tgacctccgg cgatgcgcac ctggcggtga atttcccgct gctgctggaa
    11041 aaaggcttga tggtctgcgc gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg
    11101 tactggaaga tctgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag
    11161 tcagtgaaca cattgaacgt ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc
    11221 gcgaaagccg tcgcgatgaa ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc
    11281 agccgccgca gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc
    11341 agatcgaatc taacggtcac tcagtatcgt ttggtcgtat ggaccagtat ctctacccgt
    11401 actatcgccg cgacgttgaa ctcaaccaga cactggatcg cgaacacgcc atcgaattgc
    11461 tgcatagctg ctggctgaaa ctgctggaag tgaacaaaat ccgctcaggc tcacactcaa
    11521 aagcctctgc gggaagtcct ctgtatcaga acgtcaccat tggcggacaa aatctggttg
    11581 atggtcaacc aatggacgcg gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc
    11641 gcctgcgttc cactcagcct aacctcagcg tgcgttacca cgcgggaatg agcaacgatt
    11701 tcctcgacgc ctgcgtacag gtgatccgtt gcggcttcgg gatgccggcg ttcaacaacg
    11761 acgaaatcgt catcccggaa tttattaaac tcggtattga accgcaggat gcttacaact
    11821 atgcagcgat tggttgtatc gaaaccgccg tcggtggcaa atggggctat cgctgtaccg
    11881 gcatgagctt tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg
    11941 ccaccagcgg caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca
    12001 acttcgatga agtgatggac gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa
    12061 tcgaaatcga atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct
    12121 cggcgctggt ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat
    12181 atgactgggt ttctggcctg caggttggca ttgccaacct cggcaacagc ctggcggccg
    12241 tgaagaaact ggtatttgaa cagggcgcga ttagtcagca acagctggct gccgcactgg
    12301 cgaatgactt cgacggcctg actcacgagc agctgcgtca gcggctgatt aacggtgcgc
    12361 cgaagtacgg caacgacgat gatactgtcg atacgctgct ggctcgcgct tatcagacct
    12421 atatcgacga actgaaacag taccataacc cgcgctacgg tcgtggtccg gttggcggca
    12481 actattacgc gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg
    12541 caacgccaga cggacgtaaa gcccaccccc cgctggcaga aggcgcaagc ccggcttccg
    12601 gtactgatca tcttggccct actgcggtca ttggctcggt gggtaaactg cctacggcag
    12661 cgattctcgg cggggtgttg ctcaaccaga aactgaatcc agcaacactg gagaacgaat
    12721 ctgacaagca gaaactgatg atcctgctgc gcaccttctt tgaagtgcat aaaggctggc
    12781 atattcagta caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catcccgatc
    12841 agtatcgcga tctggtagtg cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc
    12901 cagacgctca ggacgatatc atcgcccgta ccgaacatat gctgtaatcc cctcagcccg
    12961 gcggagacgt cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga
    13021 aatataattt gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc
    13081 gggagcacac ctatgagcgt aaaagttatc gtcacagata tggacggtac ttttcttaac
    13141 gacgtcaaaa cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc
    13201 ggcattgagt tcgttgtcgc cagcggtaat cagtattacc agcttatttc attctttcct
    13261 gagctaaagg atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc
    13321 aagcaactgt tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg
    13381 ctaaaagata agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa
    13441 aatgcccccg aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaacctgta
    13501 aaagattatc aggagattga cgacgtactg ttcaagtttt cactcaacct gccggatgaa
    13561 caaatcccgt tagtgatcga caaactgcac atagcgctcg atggcattat gaaacccgtc
    13621 accagtggtt ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt
    13681 tcgcggttac tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc
    13741 ggtaacgatg cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg
    13801 gaaaacatta aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg
    13861 aatgtgattc aggctgtact ggataacaca tcccctttta acagctgacc tctctaccgg
    13921 agccatgcgc tccggttatc ccttcttttt acatcctgta catcacatca aatcattaaa
    13981 cttgcattaa cttatttata acttatattg tcacatgcaa atcagcatag tttcgaatga
    14041 aagttaatgc gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca
    14101 caaagcagct atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg acgtccagca
    14161 gatctttaat cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa
    14221 agcacagggc gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt
    14281 tactgcagag cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt
    14341 cccccgcgaa caagcgctag gctgggatca gtcgatgctg gactatctgg accgcaaccg
    14401 ctttgacgag gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg
    14461 tcccgagatt taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga
    14521 aatggcgaat gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca
    14581 atggtttaac ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc gtccgcccgg
    14641 aacgacctcc aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct
    14701 tccagcgtat cagcacgttt tcgctaacgt ctttaatggc aatctggcga aatacgatcc
    14761 ctggcatgcg gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg
    14821 ttccgtgttt cggacattcc agggctggac agcgctctct gatatgctgc ctagtcaggg
    14881 gttgctgcac gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct
    14941 tgatgatgtg ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc
    15001 agagcaatgg catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg
    15061 agactccgtc tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca
    15121 gggttggggc aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta
    15181 cgcgcacaag gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga
    15241 ggactatgaa acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa
    15301 gcgagcgttg ggcatggatg tttgagagtt aattgaagtg aattgatgtg ggattaagaa
    15361 aatcgattcg acgttactca gattgctgac aagtgcgctt tcttaatgcc tgataagcat
    15421 agcgcatcag gcaatcttgc gtttgtcatc agccgccgtc accccgacag tatcggggtg
    15481 acggataata acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa
    15541 gtcgatcacg cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc
    15601 acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga
    15661 ctcgatggat tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt
    15721 ctgcatcatt aacttaccaa aaagcatccg ccctttttct ccaccggaaa gcactttagc
    15781 aggctttttg atgtcgtcct ggctgaacag caaacgaccg agaatactgc gcaccgcctg
    15841 ctcgtcatcg ccttcctgct tccactggct catccattcg aacacggtca gatcattttc
    15901 aaactcatat tcgtggtcct gagcatagta accaatgcgc gcgttttcag accattttac
    15961 ggtgccgctg tccggttgca gatcgcccac cagcgttttc aacagcgttg atttaccgac
    16021 gccgttggta cccagtaccg ccagtttttc accgacttcc agcagcaggt tgagattttt
    16081 aaacagcgga ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa
    16141 cagcttctta tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc
    16201 ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc
    16261 gttggcgcta aagcggctaa cgaaagattg cagctcagca atctgcgctt tcttcttggc
    16321 gttatcggcc agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt
    16381 acccggataa acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt
    16441 aaggaagtga cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg
    16501 ttccagccag cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat
    16561 atccggatca gcaaacagcg cctgcgccag caacacacgc agcttccagc caggagcaac
    16621 ttcactcatc gggccgtagt gctgttccac tggaattccc acgccaagca acaattcacc
    16681 ggcacgagct tccgcagagt aaccgtccat ttcgccgtat ttaacttcca gatcggccac
    16741 tttatagccg tcttcttcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt
    16801 cacttcccac aactctttat gccccatgat caccgtatcc agcacagtga actcttcaaa
    16861 ggcaaactga tcctgacgca gtttaccaat gcgctcgttg ggatcgaggg aaacgttacc
    16921 cagcgtcggc tcaaggtcgc cgccgaggat cttcataaag gtggatttac cactaccgtt
    16981 cgcgccaatc aggccgtaac ggttgccgcc gccaaatttg acggaaatgt tttcaaacaa
    17041 cggcttactg ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa
    17101 agagatatga caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt
    17161 aagatctaca ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt
    17221 gagctggaat gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg
    17281 ccaggcacaa aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct
    17341 tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg
    17401 ggagtcgcct ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc
    17461 tggagtattt tgccgcggag taccagatgg ggctttccaa tatgatggaa gcgaacccgg
    17521 gtgtggatac cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc
    17581 tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact
    17641 atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag
    17701 atacgcctat caactggacc accaaagttg agcgtaagaa agcaggcccg acctggacgc
    17761 cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga accgcttccg gctgtcgttc
    17821 cggcaggtcc ggataacccg atggggctgt atgcactcta catcggtcgc ctgtacgcta
    17881 tccatggcac caacgctaac ttcggtatcg gtctgcgtgt aagtcatggt tgtgtgcgtc
    17941 tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt
    18001 ttattgatga gccggtaaaa gcgaccaccg agccagacgg cagccgttac attgaagtcc
    18061 ataacccgct gtctaccacc gaagcccaat ttgaaggtca ggaaattgtg ccaattaccc
    18121 tgacgaagag cgtgcagaca gtgaccggcc agccagatgt tgaccaggtt gttcttgatg
    18181 aagcgattaa aaaccgctcc gggatgccgg ttcgtctgaa ttaatcttca aaacgtaaaa
    18241 caaaggcgga ctcataatcc gcctttttta tttgccagac cttaccaggc cgggatcata
    18301 accaacaaaa catatcccac cagcgccgcc cacaacagca tcggtattga atagagatgg
    18361 aaacgccacc agatgcggcg atcgttcgcc atacgtagcg caatcaaatt tgccagcgat
    18421 ccgggtaata acccaaagcc acctacgttt accgcccatg ccagtaataa agacggcggc
    18481 acatagttca gcaacaatat agtactcggc acgttactga tcacctgcga taaaccgatt
    18541 gccgttaacc ataatccggg ttcagataaa tgactcacgt tacccaacac gccttgcaac
    18601 gctggaagct gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcagc
    18661 gtccagtcca cactcaacac cacgcgacgc gccagcagcg caaagcccgc cgccacaatc
    18721 accagtcccc acagctcttg tttgaactcc agcgccgtca gaaagacgat atacagcccc
    18781 agacaactcc acaccagccg cggtttccac tccggtgttt gcacccccgt atggtattgg
    18841 agtgcctttc cagggaaaca acaccagcac aacagcagga gcgtcagcat cattgcgcca
    18901 gccagcggtg ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata
    18961 agaatatttt gcgggttgcc aattggcgtc agtagcgaac cagcgttgac tgccagcgcc
    19021 tcaaaaataa tcagccgatt aaccgggatc tcacacagcc tttttagcgt gatagtcagc
    19081 ggaacaacaa taaacagcgt gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc
    19141 agcaccataa acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc
    19201 agcacatcaa aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag
    19261 gtgatgatgg tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa
    19321 aaacttaatc cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg
    19381 cgtaaaaaag gcaggctcat ttggcaccgt gtttctgggt aaacaaacga aaggcatcca
    19441 gcgtctcttc actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac
    19501 tgacgcccaa caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg
    19561 ccagcttctc tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc
    19621 ccatagttgc cagtcgctta agcattttag ccaccgtcgg ttgcgaaact cccaaacgag
    19681 cagccatgtc tacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa
    19741 cgtaatcatc aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt
    19801 gctcttcctc gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac
    19861 tcattgtgct tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg
    19921 taaaccagag ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc
    19981 ctgtgctata tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca
    20041 ggaggtctta tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa
    20101 gtacggttaa tgctgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat
    20161 aaaccttccg ataaatagcg gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc
    20221 acgacgcgtt ttggtctgaa aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa
    20281 agatttgttc gtcagtagtt gacctgaact gcggctcgct ctatcttctt gcagccctgc
    20341 gtatattgcg gctcgcggat gcggacccct ttcccctctt cacgcactct tgcaggtatt
    20401 gacccttgac gccagggtaa gcacatggcg tttgttacga tagtggcata tgaatttaac
    20461 cctcaaagaa tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt
    20521 tttacagtcg ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc
    20581 tgcgtttacg gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct
    20641 ggtcggtgtc agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc
    20701 gcctaatttc aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat
    20761 cctgacgatt tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt
    20821 aatagcaatc aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct
    20881 gtgtctggta ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg
    20941 tggcgtattt aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat
    21001 cgtcaataat aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa
    21061 agatacctgg acggtcactg ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg
    21121 tgaaagcgcg cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt
    21181 tgccagcagc gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca
    21241 gaaatcgctt ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa
    21301 ctttgtcacc ctggcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca
    21361 aatcggcgaa tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt
    21421 cctgaaagaa ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat
    21481 gaccgctcaa gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat
    21541 gggctcgcat ccgcaggcct gcgaccggac aaaaggcaaa tacgaaacct ttgtgcaatc
    21601 gaaagaaacg tcgtgctatc tctatactat gacgcaaacg gacgatttac tgcgcaagct
    21661 gtacgatcag ttacgcaaca gcggcagcag cttctcgctg gtttactttt ctgaccacgg
    21721 tctggccttt aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataagtatca
    21781 gcaaaatttc caggtgcctt ttatggtcat ttccagcgac gataaagcgc atcgggtgat
    21841 taaagcccgc cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa
    21901 agcgaaggaa attaacatca aatacccgtt tatatctgag aagaaagccg ggccgatata
    21961 catcaccaac ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga
    22021 cccgaagcct taaaaacaaa aatccgcccc gagaggcgga ttttttatat caccaaagtg
    22081 attagaagcg gtaaccaaca ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc
    22141 tctgctcgta agagaagtcc agagcaacgt tttccatcgg gttgaactgc agaccagcac
    22201 cgtaggagaa accgtagtcg ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga
    22261 atttaccata acccacaccc actacaccgt agatgcttgc ccagtcgtta atgcggtaag
    22321 ccggaccagc agtgatgccg tagtactggt ttttgttgta gtcaccagag cttgcagtac
    22381 ggcttttctc ggtgtaagtg aatgaaccga tcacacccag cgggctgttg tcttcttcat
    22441 agcggtattt caggttgaaa ccgcccattt tgttcatttg gccctgagcg tcgctctgtg
    22501 cgtaaccgcc agttacggta gaagtcgcag ctacggaagt acctgcggtg aaagccagaa
    22561 ctgcggccag tgctgaaaga cat
//
LOCUS       DBFIFS010000054        22537 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.054, whole genome shotgun sequence.
ACCESSION   DBFIFS010000054 DBFIFS010000000
VERSION     DBFIFS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.054"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(398..1489)
                     /gene="ychF"
                     /locus_tag="N7A07_001930"
     CDS             complement(398..1489)
                     /gene="ychF"
                     /locus_tag="N7A07_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU2476602.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1606..2190)
                     /gene="pth"
                     /locus_tag="N7A07_001931"
     CDS             complement(1606..2190)
                     /gene="pth"
                     /locus_tag="N7A07_001931"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU2476603.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2468..2746
                     /gene="ychH"
                     /locus_tag="N7A07_001932"
     CDS             2468..2746
                     /gene="ychH"
                     /locus_tag="N7A07_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU2476604.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2801..4480)
                     /gene="dauA"
                     /locus_tag="N7A07_001933"
     CDS             complement(2801..4480)
                     /gene="dauA"
                     /locus_tag="N7A07_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU2476605.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4605..5552)
                     /gene="prs"
                     /locus_tag="N7A07_001934"
     CDS             complement(4605..5552)
                     /gene="prs"
                     /locus_tag="N7A07_001934"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU2476606.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5703..6554)
                     /gene="ispE"
                     /locus_tag="N7A07_001935"
     CDS             complement(5703..6554)
                     /gene="ispE"
                     /locus_tag="N7A07_001935"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU2476607.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6554..7177)
                     /gene="lolB"
                     /locus_tag="N7A07_001936"
     CDS             complement(6554..7177)
                     /gene="lolB"
                     /locus_tag="N7A07_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU2476608.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7391..8647
                     /gene="hemA"
                     /locus_tag="N7A07_001937"
     CDS             7391..8647
                     /gene="hemA"
                     /locus_tag="N7A07_001937"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU2476609.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8689..9771
                     /gene="prfA"
                     /locus_tag="N7A07_001938"
     CDS             8689..9771
                     /gene="prfA"
                     /locus_tag="N7A07_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU2476610.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9771..10604
                     /gene="prmC"
                     /locus_tag="N7A07_001939"
     CDS             9771..10604
                     /gene="prmC"
                     /locus_tag="N7A07_001939"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU2476611.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10601..10993
                     /gene="sirB2"
                     /locus_tag="N7A07_001940"
     CDS             10601..10993
                     /gene="sirB2"
                     /locus_tag="N7A07_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU2476612.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            10997..11806
                     /gene="sirB1"
                     /locus_tag="N7A07_001941"
     CDS             10997..11806
                     /gene="sirB1"
                     /locus_tag="N7A07_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU2476613.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11842..12696
                     /gene="kdsA"
                     /locus_tag="N7A07_001942"
     CDS             11842..12696
                     /gene="kdsA"
                     /locus_tag="N7A07_001942"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU2476614.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12844..12951)
                     /locus_tag="N7A07_001943"
     CDS             complement(12844..12951)
                     /locus_tag="N7A07_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2476615.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13380..13487)
                     /locus_tag="N7A07_001944"
     CDS             complement(13380..13487)
                     /locus_tag="N7A07_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2476616.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13886..14986)
                     /gene="chaA"
                     /locus_tag="N7A07_001945"
     CDS             complement(13886..14986)
                     /gene="chaA"
                     /locus_tag="N7A07_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU2476617.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15256..15486
                     /gene="chaB"
                     /locus_tag="N7A07_001946"
     CDS             15256..15486
                     /gene="chaB"
                     /locus_tag="N7A07_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU2476618.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15647..16342
                     /gene="chaC"
                     /locus_tag="N7A07_001947"
     CDS             15647..16342
                     /gene="chaC"
                     /locus_tag="N7A07_001947"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU2476619.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16386..16739)
                     /gene="ychN"
                     /locus_tag="N7A07_001948"
     CDS             complement(16386..16739)
                     /gene="ychN"
                     /locus_tag="N7A07_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU2476620.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLALRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16925..18319
                     /gene="ychO"
                     /locus_tag="N7A07_001949"
                     /gene_synonym="iatA"
     CDS             16925..18319
                     /gene="ychO"
                     /locus_tag="N7A07_001949"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU2476621.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18320..18970)
                     /gene="narL"
                     /locus_tag="N7A07_001950"
     CDS             complement(18320..18970)
                     /gene="narL"
                     /locus_tag="N7A07_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU2476622.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18963..20759)
                     /gene="narX"
                     /locus_tag="N7A07_001951"
     CDS             complement(18963..20759)
                     /gene="narX"
                     /locus_tag="N7A07_001951"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU2476623.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21098..22489
                     /gene="narK"
                     /locus_tag="N7A07_001952"
     CDS             21098..22489
                     /gene="narK"
                     /locus_tag="N7A07_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU2476624.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5521 a         5631 c         5852 g         5533 t
ORIGIN      
        1 atcgttgtta ttgtgctact ggttatcatt ttcatcttgc caggatcgca cgtaatcacg
       61 ttattcgggg actttatctg cgggatatta cccttcaggt aagtcctttt ctcgccagca
      121 tattggagcg tggtcaatat tgatcggctg tgaagggggc tgatgtgtag tggaacaaaa
      181 actgacgtta tatcactgct aatgagatat ccgtcatctg aacactgatg tctttaatta
      241 ttctcgtggt tcactgtagg caataagcac aaaagtgtag gatgttacaa gaatgattag
      301 gactcggtga aatgaaaaat ccacgcaatt gcgtggattt tatatacttt tgcgctcttc
      361 atgagattta gcgaaacctc atgagacaat aaattaatta gacgttgaaa aggaagttca
      421 tcacatcgcc atctttcacg atgtaatctt taccttctgc acgcattttg cctgcttctt
      481 tcgcgccttg ttcacctttg taagtgatga aatcttcaaa cgagatggtt tgtgcacgga
      541 taaagccttt ttcaaaatca gtatggattt tgcccgctgc ctgcggcgcg gttgctccaa
      601 ccggaatggt ccatgcacgc acttctttca ccccagcggt gaagtaagtt tgcaggttca
      661 gcagcttata accggcacgg atcacacggt tcagacccgg ctcttccagc ccaagctcct
      721 gcataaactc gtcacgttct tcgtcgtcca gttcggcaat gtctgcttca acagcagcac
      781 aaaccggaac cacaacagaa ccttctttcg ccgcgatttc acgcacctgg tcaagatatg
      841 ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta cattgttggt ttcagcgtca
      901 ggaagctcag gtaacgaata gccgctttct cttcagcgct taaatccagc gcgcgcagca
      961 tacctgcgtt ttccagctgg ggcaggcatt tttccaggac cgccagctca gctttcgcgt
     1021 ctttatcgcc acctttggct ttcttctgta cgcgatgaat cgcacgttcg caggtgtcga
     1081 ggtctgccag cgccagttcg gtgttgataa cttcaatatc gtcagccggg ttaactttgc
     1141 cggaaacgtg aatgatgttg tcattttcaa agcagcgaac aacgtgaccg atcgcttcgg
     1201 tttcacggat gttggtcagg aactggttac ccagaccttc gcctttcgat gcgcctttta
     1261 ccagaccggc gatatcgaca aattccatgg tcgtgggaag cgtacgttgc ggttttacga
     1321 tttcagccag ttgatccagg cgaggatcag gcattggtac gacgcctgtg ttcggctcaa
     1381 tggtgcagaa tggaaagttg gccgcttcaa taccggcttt ggtcagcgcg ttgaacaggg
     1441 tagatttccc gacgttgggc aaaccgacga taccgcattt gaatcccatg atttaaatca
     1501 ccttaatatc ttaataatca acctgttatt gctaacagat tgcagaaatg gaaataactt
     1561 tgcctattat acacggcact cggcaaaaat gccgcagaca acgacttatt gcgctttaaa
     1621 ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta aaccacattt cagtacaacg
     1681 cgccgcttcg tcgatggctt catcaattaa cttctgttca ctaacaggcg gtttgcctaa
     1741 cacaaaacca acaactttgt ttttatcgcc cggatgaccg attccgatgc gtaaacggtg
     1801 aaagttaggg ttattaccca atttactgat gatgtctttc agtccattgt gaccaccatg
     1861 gccaccgccc aacttaaatt tggcgacgcc aggaggcaga tccagttcgt cgtgggccac
     1921 cagaatttcg tccggattaa tgcggaaaaa actggccatc gccgcaacgg ctttgccgct
     1981 gagattcata aatgtagtcg ggactaacag gcggacatct tcgcctccaa gggtgactcg
     2041 cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga gcgcgcaaac gctctgccag
     2101 taagtcaacg aaccaggcac cagcattatg tcgcgttgcg gcgtattcag caccggggtt
     2161 cgccaggccg acaatcaatt taatcgtcac gtttttttgt cctgagtgtg tacataactg
     2221 gcgcgtagtt tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa atgcagataa
     2281 cgtaataatt gcctgagtgg actattagaa agtcaaggtg ttcaggcgtt tatttgtaaa
     2341 gttttgttga aataagggtt gtaattgtga tcacgcccgc acataaccca cagggtgttg
     2401 tctatacttt acacataagg aagaggggta ttccctgtta caacccagaa agttccggag
     2461 gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg ggttgggtct
     2521 ggtggtaatg gtggtcggcg tggggtattc aatcctcaac cagttaccac agtttaatat
     2581 gccccagtat ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca ttctctggct
     2641 ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt gggttcgcca
     2701 ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa taatgcagtt
     2761 tgctgaccag tcagtttgcg ctgactggtc aatctcgtac ttataaatcc gccatcgccg
     2821 cgcgacgatt cgggaagaac gcgaggcgtc ccgggatcgg ttgaatgcct gcgcgagcca
     2881 tagtgcgcag tggctggaat tccacgttgc acacgcgcag ttcacatcct tcgggcagac
     2941 gcttcacaaa acgctggaac gcatcaagac caccagcatc aagtaccgga acggcatccc
     3001 acttcagaat cacaatccgt ttgccttcaa gacgtgactc caggtccgtg aataagcctt
     3061 cagcagcagc aaaaaacagc gggccaataa cgcgcagaac caggacatcg tctggaacat
     3121 ctacgactac cggtgccagg cgagtcatac gtgcgatacg acgcataaac agcagcgatg
     3181 ccagcacgat ccccacgctg atggcaataa ccatatcaaa cagcacggtc agcgacatgc
     3241 acagcagcat gacaatgatg tcatctttcg gtgcatgacg cagcaagtcg accactttat
     3301 gcgcttcact catgttccac gccaccatca acagcagggc tgccatagcg gaaagcggca
     3361 gccaggagag cagcggtgcc agtaccagca gggcaagaat aaccagaata gagtggatca
     3421 ccgccgagat aggggaagtt gccccggcac ggacgttagc ggcagaacgc gcgatggcag
     3481 cggtagcggt aataccacca aagaacggag cgatgatatt ccccagcccc tgtccaacca
     3541 gttcgctatt cgccttgtgc ttcgtcccgg tcataccatc cagcaccacg gcgcagagca
     3601 gagattcgat tgcgccgagc attgccattg agaatgccgc aggcagcagt gtgcgaatag
     3661 aatcccaggt tagcgtgaat tctgaattag gcagatccca cggcagcacc agttgcggta
     3721 gcagttgcgg aataccgtta ccctgagaac catcggccag gacgtagtgg aattgcgaac
     3781 cgatggtagc aacatgtccg ccgagcaggt taacaatccc catcaccgcg caaccagcca
     3841 gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg cggccagaaa acaagaatac
     3901 ctagcgtcac aatgccaatg gcagcatcac ccacattaat ggtcggcagc gccataaata
     3961 atgcgccgac tttttgtaga taatgttccg ggacatgggc catttgcaga ccgagaaaat
     4021 ctttaatctg catggtaccg atggtgatcc cgatacccga ggtgaaacct aaggtgacgg
     4081 aaaccggaat atactcaatc aggcgaccaa agcgtgccag acccatcaga atcaaaaaga
     4141 tccccgacag caaggtcgca accagcagtc ctgccagccc aaactgttgc gaaacgggat
     4201 agagaattac cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac ccacccgtca
     4261 gagcaatgac aatccccgca acagctgcgg tatataaacc gtactggggt gccacaccac
     4321 taccaatagc caacgccatc gccagcggga tagcaataat cccgacggtt atcccggcaa
     4381 tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg tcgatcagag
     4441 cgcggaaagg catcacatgt gaggaaaata ttttgttcac aataatgttt catccgtgag
     4501 cgcatcatct gtcaactaaa tggcaggtga aggaggcata ggtcatacaa atggatatta
     4561 cagacaaaaa aacccgccgc agcgggtctt tgagccgggt tcgattagtg ttcgaacatg
     4621 gcagagatcg attcttcgtt gctgatacga cgaatcgctt cggccagcat acctgacagg
     4681 gtcagagtac gcacgttcgg cagtgatttg atttcatcgc tcagcggaat ggtatcgcag
     4741 acaacgactt catcaattac agagttacgc aggttgttcg ccgcgttgcc agagaagatc
     4801 gggtgagtcg cgtacgcaaa tacacgttta gcaccacgtt ctttcagagc ttcagcagct
     4861 ttacacagcg taccgccagt gtcgatcata tcatcgacca gtacgcagtc acgacctgca
     4921 acgtcaccga tgatatgcat cacctgggaa acgttcgcac gcggacgacg tttgtcgatg
     4981 attgccatat cggtatcgtt cagcagctta gcgatagcgc gggcacgcac aacgccgccg
     5041 atgtccggag aaaccacaat tgggttatcc agattcagct gcagcatgtc ttccagcagg
     5101 atcgggctac caaatacgtt atcaaccgga acgtcgaaga aaccctgaat ctgttcagcg
     5161 tgcagatcca ctgtcagcac acggtcaaca ccgacgctgg agaggaagtc tgcaaccact
     5221 ttcgcagtga ttggtacacg agcggaacgg acgcgacggt cctggcgcgc atagccaaag
     5281 taggggataa cagcggtgat acgacctgcg gaagcacgac gcagggcatc aaccataacg
     5341 actaattcca tcaggttgtc gttagtaggg gcacaagtgg actggatgat gaaaatatca
     5401 ccaccgcgta cattttcatt aatttgtacg ctgacttcgc catcgctaaa gcgacctaca
     5461 gcggcgtcgc cgagtgaagt gtacaggcgg ttggcaatac gttgtgctag ttccggggtg
     5521 gcgttaccag caaaaagctt catatcaggc acgagaagaa cctcaggcat gcgtccattg
     5581 gtggaaagaa tctgccgaaa actgtgcggg ccaggcatga tcctttccag gcggtgtatt
     5641 aaagagcgcg atgcaacgtc tggaacaagg tgacgttgtc accgaaactc agcttgcccg
     5701 gcttaaagca tggctctgtg caatggggaa agattagcgc ctttcgccac aaagccattg
     5761 agccattccg gggcttgctc tagcacctgg cgggcttcag actctgtatc aaattcagca
     5821 aagacacagg cccctgtccc agtcaggcgc gacggggcgt attctaacag ccaggaaagc
     5881 accgcatcaa cctcgcgaaa acgttttctt gcgataacct cgcaatcatt gctgaattca
     5941 cattttagca acgtttctat tgaccttttt ggcgtattgc gcgggagttc aggatcttta
     6001 aaaatcaccg gagtcggaat acttacacca gggtgcgcca ccagatacca cttctctggc
     6061 ggatccaccg gggttagtat ttcaccaacg ccttcggcaa acgcggcatg cccccgaaca
     6121 aagacaggaa catctgcgcc cagcgtcagc cccatttccg ccagctcatc catgcttagc
     6181 ccgcattgcc agagatgatt taatgccacc aggaccgtcg cggcattgga tgaaccaccg
     6241 ccgagaccgc cgcccatcgg caaacgcttg tcaatgctga tattcgcacc gcttcccgtc
     6301 ggaagacgcc cgctgtctgc cgcagttttc atcaacaacc gcgctgcgcg aacgatcagg
     6361 ttatcttcat gttccacgcc ttcaacgggc gttaacagac ggatatcccc atcgtcacga
     6421 agctcaatgc tgatggtgtc gccgtaatca agaaactgaa acagcgtttg cagcgtgtgg
     6481 taaccatccg cacgctgacc ggtaatgtat aaaaacagat taagttttgc cggagagggc
     6541 cactgtgtcc gcattatttc actatccagt tatccatttt taacttgatg cgttgaccac
     6601 cgtcggtgag ttccatattg gctggcatcg caggttgcgt tttggtgtca taaccaccat
     6661 aaacaacctt ccagtttttg ccattctggc tgtaggtaat ttcgctcagg cggtactggt
     6721 cgtccagttt gtagtcggtt gcatcacccg gtaaacctaa aatccactgg cgcaagctgt
     6781 tgagcggaat tggcattcca gtcaatttgc caatcatctc ttcggcgtca tcggcggtat
     6841 aacgctgacc tttattgtcg actaactgca cgttacccgg ttgagcattc agctccagtt
     6901 ccgtgctgcc caatgggtta gtgagcagca gacggtagcg atcctggccg gtttgctgcc
     6961 agaaaaagcg ggcgtacact ttttgttggt cagaaatata agcgaacgcg ccgcgagtct
     7021 gatactgatt aagattgcgc acgtcttgct gatgctgacg ccattgtggc gaatccgggc
     7081 ttttgccagg acctttgggc gtggtaacgg aacaggcagt gagcacaaga gcagccagcg
     7141 gtagcaggcg gataagacga aaatcgggca ggggcatagt gatgacaagt ccttgagata
     7201 cgttgcagtt ataaccctta atgctagcgt caccattagt catcgtctat gttcaagttg
     7261 tcttaattgt agaaatctaa cgggttcggg caattactcc aaaagggggc gctccctttt
     7321 attgatctcg cgcatacggt atgatgcgcc gagactaacc ttatcaacgt tggtattatt
     7381 tcccgcagac atgacccttt tagcgctcgg tattaaccat aaaacggcac ctgtatcgct
     7441 gcgagaacgt gtatcgtttt cgccggacaa acttgatcag gcgctggaca gtctgctcgc
     7501 acagccgatg gtgcagggcg gggtggtgct gtcgacgtgt aaccgcactg agctttatct
     7561 tagcgtcgaa gagcaggaca acttgcaaga ggcgttaatc cgctggcttt gcgattatca
     7621 caatcttaat gaagaagatc tgcgtaaaag cctctactgg catcaggata acgacgcggt
     7681 tagccattta atgcgtgttg ccagcggcct ggattcactg gttcttgggg agccgcagat
     7741 cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa ggtcatatga aggccagcga
     7801 actggaacgc atgttccaga aatctttctc tgtagcgaaa cgcgtacgca ctgaaacaga
     7861 tatcggtgcc agcgctgtgt ctgtcgcttt tgcggcttgt acgctggcgc ggcagatctt
     7921 tgaatcgctc tctacggtca cagtgttgct ggtaggcgcg ggcgaaacca tcgagctggt
     7981 agcgcgtcat ctgcgcgaac ataaagtaca gaagatgatt atcgccaacc gcactcgcga
     8041 acgtgcccaa attctggcag atgaagtcgg cgcggaagtg attgccctga gtgatatcga
     8101 cgaacgtctg cgcgaagccg atatcatcat cagttccacc gccagcccgt taccgattat
     8161 cgggaaaggc atggtggagc gcgcattaaa aagccgtcgc aaccaaccaa tgctgttggt
     8221 ggatattgcc gttccgcgcg atgttgagcc ggaagttggc aaactggcga atgcttatct
     8281 ttatagcgtt gatgatctgc aaagcatcat ttcgcacaac ctggcgcagc gtaaagccgc
     8341 agcggttgag gcggaaacta ttgtcgctca ggaaaccagc gaatttatgg cgtggctgcg
     8401 agcacaaagc gccagcgaaa ccattcgcga gtatcgcagc caggcagagc aagttcgcga
     8461 tgagttaacc gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc aagccattat
     8521 gcaggatctg gcatggaaac tgactaaccg cttgatccat gcgccaacga aatcacttca
     8581 acaggccgcc cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca gcctcgggct
     8641 ggagtagcag tacatcattt tcttttttta cagggtgcat ttacgcctat gaagccttct
     8701 atcgttgcca aactggaagc cctgcatgaa cgccatgaag aagttcaggc gttgctgggt
     8761 gacgcgcaaa ctatcgccga ccaggaacgt tttcgcgcat tatcacgcga atatgcgcag
     8821 ttaagtgatg tttcgcgctg ttttaccgac tggcaacagg ttcaggaaga tatcgaaacc
     8881 gcacagatga tgctcgatga tcctgaaatg cgtgagatgg cgcaggatga actgcgcgaa
     8941 gctaaagaaa aaagcgagca actggaacag caattacagg ttctgttact gccaaaagat
     9001 cctgatgacg aacgtaacgc cttcctcgaa gtccgagccg gaaccggcgg cgacgaagca
     9061 gcgctgttcg caggcgatct gttccgtatg tacagccgtt acgccgaagc ccgccgctgg
     9121 cgggtagaaa tcatgagcgc cagcgagggt gaacatggtg gttataaaga gatcatcgcc
     9181 aaaattagcg gtgatggtgt gtatggtcgt ctgaaatttg aatctggcgg tcatcgcgtg
     9241 cagcgtgttc ctgctacgga atcgcagggt cgtattcata cttctgcttg taccgttgcg
     9301 gtaatgccag aactgcctga cgcagaactg ccggacatca acccagcaga tttacgcatt
     9361 gatactttcc gctcgtcagg ggcgggtggt cagcacgtta acaccaccga ttcggcaatt
     9421 cgtattactc acttgccgac cgggattgtt gttgaatgtc aggacgaacg ttcacaacat
     9481 aaaaacaaag ctaaagcact ttctgtactc ggtgctcgca tccacgctgc tgaaatggca
     9541 aaacggcaac aggccgaagc gtctacccgt cgtaacctgc tggggagtgg cgatcgcagc
     9601 gaccgtaacc gtacttacaa cttcccgcag gggcgcgtta ccgatcaccg catcaacctg
     9661 acgctctacc gcctggatga agtgatggaa ggtaagctgg atatgctgat tgaaccgatt
     9721 atccaggaac atcaggccga ccaactggcg gcgttgtccg agcaggaata atggaatatc
     9781 aacactggtt acgtgaagca ataagccaac ttcaggcgag cgaaagcccg cggcgtgatg
     9841 ctgaaatcct gctggagcat gttaccggca gagggcgtac ttttattctc gcctttggtg
     9901 aaacgcagct gactgacgaa caatgtcagc aacttgatgc gctactgaca cgtcgtcgcg
     9961 atggtgaacc cattgctcat ttaaccgggg tgcgagaatt ctggtcgttg ccgttatttg
    10021 tttcgccagc gaccttaatt ccgcgcccgg atacggagtg tctggtggag caggcactgg
    10081 cgcggttgcc tgaacaacct tgccgtattc tcgatctcgg gacgggtacc ggggcgattg
    10141 cgcttgcgct ggctcgcgag cgcccggact gcgaaattac cgctgtagat cgtatgcccg
    10201 atgctgtcgc cctggcacaa cgtaatgccc agcatctggc gatcaaaaat atccacattc
    10261 tgcaaagcga ctggtttagc gcgctagccg ggcagcagtt tgcgatgatt gtcagcaatc
    10321 cgccgtatat tgacgagcag gaccctcatc ttcaacaagg cgatgtccgc tttgagccgc
    10381 tcactgcgct ggttgcggca gacagtggaa tggcagacat cgtgcatatc atcgaacagt
    10441 cgcgtaacgc gctggtatcc ggcggctttc tgcttctgga acatggctgg cagcagggcg
    10501 aagcggtgcg acaggcattt atcctcgcgg ggtatcatga cgtcgaaacc tgccgtgact
    10561 atggtgataa cgagcgcgta acgctcggcc gctattatca atgacaagtt tttctacact
    10621 gcttagtgtt catcttatta gtatcgcgct ttctgttggg ctattaacct tacgtttctg
    10681 gctacgttat cagaagcatc cacaggcatt tgctcgctgg acgcgcattg tgccgccggt
    10741 tgtcgatacg gtgttattgt taagcggcat tgcgttgatg gctaaagcgc acatcctgcc
    10801 attttccggg caggcacagt ggctgactga aaagctgttt ggagttatca tttatatcgt
    10861 tttgggtttt attgcactcg attatcgtcg tatgcacagt cagcaggcgc gcattattgc
    10921 cttcccgctg acgttggtgg tgctgtacat catcattaaa ctcgccacca caaaagtacc
    10981 gttactgggg taagtcatga gatcgttagc tgatttcgaa tttaataaag cgccattgtg
    11041 cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat tttccctcgc aagatgttta
    11101 cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa atcagccagc ttctgccttt
    11161 agaagagcag ctggaaaaac tgatcgcgct gttttacggc gactggggat ttaaagcctc
    11221 acgcggcgtt tatcgtcttt ccgatgcatt atggctggac caggtgttaa agaatcgaca
    11281 gggcagtgcg gtatcattag gtgcggtttt attatgggtc gcgaatcgtc tcgatttgcc
    11341 gctgctgccg gtgattttcc ctacgcagct gatattgcgc attgaatgtc cggatggcga
    11401 aatttggctg attaatcctt ttaacggtga atcgttaagc gaacatatgc tggacgtatg
    11461 gttaaaggga aatatcagcc cgtcggcgga actgttttat gaagaccttg atgaagctga
    11521 taacattgag gtaatccgca aattgctgga tacactcaaa gcctcgttga tggaagaaaa
    11581 tcagatggag ctggcgttac gcaccagcga agctttatta caattcaacc cggaagatcc
    11641 ctatgaaatt cgcgatcgcg ggttgattta tgcgcaactg gattgcgaac acgttgcgtt
    11701 gaacgattta agttatttcg ttgaacagtg tccggaagac ccgatcagcg aaatgatccg
    11761 tgcgcaaata aataacatcg cgcataaaca tattgtgctg cattaattaa tcgacatttt
    11821 actcaaggtt aaggcgatcc tatgaaacaa aaagtggtta gcattggcga catcaacgta
    11881 gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg tgctggaatc tcgcgatctg
    11941 gcgatgcgca tttgcgagca ctacgtaacc gtgacccaga aactgggtat cccttacgtg
    12001 ttcaaagcct cttttgacaa agccaaccgc tcctccatcc actcttatcg tggaccgggc
    12061 ctggaagaag ggatgaaaat cttccaggag ttgaagcaga cttttggcgt gaaaattatc
    12121 accgacgttc acgaaccaag tcaggcacag cccgttgctg atgtcgtgga tgtgattcag
    12181 ttgccggcgt ttcttgctcg ccagactgac ctggttgaag ccatggcgaa aaccggtgcg
    12241 gtaattaacg tcaagaaacc acagtttgtc agcccgggac agatgggtaa tatcgttgat
    12301 aaattcaaag aaggcggcaa cgaaaaagtg attctttgcg atcgtggtgc taacttcggc
    12361 tatgacaacc tggttgtcga tatgctgggc ttcagcatta tgaagaaagt gtctggtaac
    12421 tcgccggtga ttttcgacgt gacccacgca ttgcaatgcc gcgatccttt tggcgcagct
    12481 tccggcggtc gtcgtgctca ggtggctgag ctggcacgag ccggtatggc ggtaggtctg
    12541 gcggggctgt ttattgaagc gcatccggat ccggaacatg cgaaatgtga tggtccatcc
    12601 gcgctgccgc tggctaaact ggaaccgttc ctcaggcaga tgaaagcgat tgatgatctg
    12661 gtgaaaggtt tcgaagaact ggataccagc aagtaatctt ttttgcttga aaaataaagt
    12721 cttagcgttc tgcgttaaga cttttttcat gggtgccgga tacaaaaaag gccgcaggct
    12781 gttaccctgc ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc
    12841 gccttacttc cggttacgcc accagccgac aatcgctgcg gtaataattc ccgccaggat
    12901 cggtgctgcc aggtcgtgcc agaaaatcat ggcaaactgc gcgagcgtca tatagccgcc
    12961 ttgttgtaat gacaacattt tgcggctatt cttgaattgt tgatggttca agattagccc
    13021 ccgttctgtt gtcaggtttt acctctcaac gtgcgggggt tttctctttc cagcaaccaa
    13081 tgccaccagg gataaagccc ccgcaacatt gcgcctcacc ggataacgcc ggcttggtgt
    13141 ggatactacg actcaattca tcttcacttc atccctgaaa tgtttgcaat gaagagtgca
    13201 ttccggtttt tcaacagctg ttacagtcgt ttcatgagtg ctctggatga tgcttccagc
    13261 tcgggttgcc aatatttact tgtggaagtg ataaagacaa aaatggccgc aggctgttac
    13321 ccctgcggcc ggtttcgggc gcatattgcc atcacggcag cctgacgccc gttttcacct
    13381 tacttccggt tacgccacca gccgacaatc gctgcggtaa taattcccgc caggatcggt
    13441 gccgccaggt cgtgccagaa agtcatggca aactgcgcga gcgtcatata gccgccttgt
    13501 tgtaatgaca acattttgcg gctattcttg aagtgtctgg tttcaagatt agccctgttg
    13561 tcaggtttta cctctcaacg tgcgggggtt ttctctttcc agcaaccaat gccaccaggg
    13621 ataaaacccc cgcaacattg cgcctcaccg gataacgccg gcttggtgtg gatactacgt
    13681 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
    13741 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
    13801 acctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatattatc tcccccggtt
    13861 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
    13921 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
    13981 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgcaatt
    14041 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa attgttgcta
    14101 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
    14161 atgcacctaa accttccggc gacagaatca acagtgccac caggaagcca gtaaaggcga
    14221 caggggcatt catgctgtcg agcaaagtct ccagcgggct ggcgttcatt ttggtgactg
    14281 caataacggc aatcaggtgg ataatcaacc agatagcatg ccacaggctg ctatgggcag
    14341 acggtttacc gtggtgcggg tcgtcgtcat cactgtcatc ttcgtgctcg tagacaaaca
    14401 aactttgatg cgttttggtc tggatcagca aaaatacacc atacattgcc gcagaaatta
    14461 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
    14521 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttaata ccaaacagat
    14581 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagccg accagcccac
    14641 cggtaacaat cataatgatc gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
    14701 cggttgccat taaagctgaa atcaaactaa cttcaagaat aaccactgaa aggctaagaa
    14761 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
    14821 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
    14881 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
    14941 aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
    15001 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
    15061 atatcggtaa cattttacga atttttaccc cgttgtcgat tttttactca ttggggcata
    15121 aaaataagta ttacgtttag gcaatgtttg ttttagcgtt tcttaagaac agtctgacat
    15181 gaaaattctt atgtttttgc aagagtagat attgttgacc acacttaatg ttcaactttg
    15241 taaaaggagt caacgatgcc gtataaaacg aaaagcgacc tgccggaaag cgtaaagcac
    15301 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
    15361 tataaagata aagaagatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
    15421 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
    15481 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgc
    15541 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
    15601 caaaaagttg tcgcagggaa gcatgcagtg gcggaggtgt aagaaggtga taacgcgtga
    15661 tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg
    15721 gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgagag
    15781 accggtgtgg atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga
    15841 gtcgtgcacc ggtacactgg ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg
    15901 gcgcgggact gcgcaccagc cgggacggat gcttgcacta aaagagggcg gacgtaccac
    15961 aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaaccc tgttgtggaa
    16021 gcgcgagatg attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg
    16081 acgcacagta aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga
    16141 cactcgcgct caggtcatcg cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa
    16201 cgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg
    16261 gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt
    16321 gttgcggccg ggattcgcct gagtaaactt ccctcatcgt ggggcgtcag acgccccctc
    16381 aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag ttccacaaga
    16441 gtaccgattt ccaccccatc aatcagagga agtgtactaa tcccgcgccc gtcggtacag
    16501 gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgtatg
    16561 ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatcagg
    16621 aacagacgca gagccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg
    16681 ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt
    16741 atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt
    16801 ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag
    16861 ttgccagtaa tcgttattct ttaaggctat gttttttcat tttttaccgg aagttaccga
    16921 cgttttgagc cgtttcgttc ctcgcattat tccgttttat ttactcttgc tggcggcagg
    16981 cggtacagct aacgcacaat ctaccttcga gcaaaaagcg gcaaatccct ttgataataa
    17041 caatgatggt ctgccggatt taggcatggc gcctgaaaat catgatgggg aaaaacactt
    17101 tgctgaaatt gtgaaagatt tcggcgaaac cagtatgaat gataacgggc tggatactgg
    17161 cgagcaggca aaagctttcg cattgggaaa agtccgcgac gcgcttagtc aacaggttaa
    17221 tcagcacgta gagtcgtggc tatcaccgtg gggaaatgcc agtgttgacg tcaaagtgga
    17281 taacgaaggt cattttaccg gcagtcgtgg aagctggttt gtgccgttac aagataatga
    17341 tcgttatctc acctggagcc agcttggtct tactcagcag gatgatgggc tggtgagcaa
    17401 tgtgggcgtt gggcaacgct gggcgcgcgg caactggctg gtgggttata acacttttta
    17461 tgacaacttg ctggatgaaa atcttcagcg agcgggcttt ggtgccgaag cgtggggcga
    17521 atatttgcga ctatcggcaa acttttatca gccgtttgct gcatggcatg aacagacagc
    17581 cacgcaggaa caacggatgg cgcgcgggta cgacctgaca gcccggatgc gcatgccgtt
    17641 ctatcaacac ctcaatacca gtgtcagcgt agaacagtat tttggtgatc gtgttgattt
    17701 gtttaactct ggtacgggtt atcacaatcc cgtcgcgttg agtctgggat taaattacac
    17761 ccctgtgcca ttagtcactg tgacggccca gcataaacag ggtgaaagtg gcgagaatca
    17821 aaataacctc gggctgaatc ttaattaccg ctttggtgta ccgctcaaaa aacaactttc
    17881 tgcgggcgag gttgccgaaa gtcagtcgtt acgtggtagt cgctatgata atccgcagcg
    17941 aaataatcta ccgactcttg agtaccgaca gcgaaaaacg ttaacggtgt ttcttgcgac
    18001 accgccgtgg gatctaaaac ctggcgaaac agtgccgctg aaattacaaa tccgcagtcg
    18061 ttacggtatt cggcaactga tttggcaggg cgatacgcag atattaagtt tgacgccggg
    18121 cgcacaagcc aacagcgcgg agggctggac gctgatcatg cctgactggc agaacgggga
    18181 aggggcgagc aatcactggc gattgtcggt ggtggtggaa gataaccagg ggcagcgtgt
    18241 ctcctccaat gagatcacgc taacgcttgt cgaaccgttc gacgcattgt caaacgacga
    18301 actgcgctgg gaaccgtaat cagaaaatgc gctcctgatg cacccatacc gctgcttcca
    18361 cgcgagactt gagcttcatt ttcttcagca tgtgcttgac gtgcactttt actgtgcttt
    18421 cggtgatgtc caggcggcgg gcaatcatct tgttcggcaa accctgggca atcagcttga
    18481 gaatatcgcg ctcgcgtggg gttaactggt taacatcgcg ctcagtcgtg gcacggttag
    18541 cgcgcaagct ggcggccaga acaggcgtta atgcttcgct taataccatt tcgccagcag
    18601 ctgcctgatg caatgctttc agcagatctt ccggttccat atcttttaac agatagccat
    18661 ccgcgccgcg tttcagtgcg gtgaccacat cttcttcatg gttagagacg ctgaatacca
    18721 caatgcgccc tgagagggac ttttcgcgca gtttatccag cgtttccaga ccgttcatgc
    18781 cgggcatatt gagatctaac aggatcagat cgggatcaag agattccgcc agttcaatac
    18841 cctgttcgcc attactcgct tcgccaacca cggtgatatc tggtgccata ctgataagct
    18901 gttttacgcc agttcgcagc atcgggtgat cgtcaatcag caggatagta gccggttcct
    18961 gattactcat gggtatctcc ttggacgtct gtgaaagttt tttcgggaat aaaggtgacc
    19021 accacttcgg tgccacctga ttcacgacgg cggacgcggc aatcgcctcg taaactttgc
    19081 gcacggtcgc gcattattat catgccgtag tgattgctgc ggatggcatt ttcaggcacg
    19141 ccgcagccgt tatcctggac ggtcagtttg acctgattat cgttttgcgc caccgtcacc
    19201 acgacctcac tcgcttgcga atgtttgagg gcgttactta atgcctcacg ggcaatttgc
    19261 aacaagtgga ttgcctgatg cgaaggcacc agacgaggcg gcaattgata atccagcttc
    19321 accgggaagc caaatttggc gctgtactct tcgcaactcg cctccagcgc cggacgtaat
    19381 ccaggctcgg tgagctgcaa gcggaatgtg gtgagcaatt cacgcaactg cgcccaggat
    19441 gcattcagtt cgttacggat ctgacttaac agttcgcggc tgctttctgg cagcgcatcg
    19501 ccctgcatct gtaaacaact cacctgcatc ttcatgcaag agagagattg ggcaatagaa
    19561 tcatgcagtt cgcgcgcaat ggtggcacgc tcttccatca cgatcaactg ttgctgacgt
    19621 tcctgatgcc gatccagcgc cagcgtggcg gtgagttgtt caaccagggt atccaccagt
    19681 tgttgttgat catggctaag atgacgcccc tgcggtaggg tcgccagcaa aataccgtac
    19741 tgcgtatgag agtcagccag ccgccacttc agggtcgtgc cgcgatcacc aacgggtaat
    19801 acgccgcgcg ggcagagctg gcagccttta tcatcacaag tcatatctgg ctggcaggta
    19861 aactcctgat gattctcttc atcatcagtg tcatacaccc gcagttcgat atcacgtagc
    19921 agggttaaat tctgtaagcc gttgagtaca ggtgacaggc gttcacacag cggggcgcgg
    19981 gaatgcaaac ggcggttagc ctgccataaa aaagagagga tctgattttt atgctccagc
    20041 ccggcggttt tctcctgaac ccgctgctca agtacggcat aactttcggc cagttctgca
    20101 gacatattgt tcaacgcagt tccaagcatc gccatttcgt tgcgcccgct gatgtttgcg
    20161 cgttgggtaa aatcgcgatg gctgacggca ctcgccattg ccagcagttg ccgccacggt
    20221 tgtagcagtc gcgcccgcaa ccagataata gtgaacacca gtaaaagtgc cataaatacc
    20281 gccattaccc gatggaccag taccactgtc tcaatgcgca tttccgtggt gcggtcaaaa
    20341 ccagatacca gctgatcaag cccggcaaca aactggctga catccgctga caccgtttcg
    20401 cggttttgtg cacgcatcag tgcagggatg agttcattac gccagtaatc ttgtaaaccc
    20461 tgtaattgcg ccagttgtcc gtctcgttct gctgctcgag tcaactcggc gctaaatgcc
    20521 gtttgttcca tctctttaat taagggcttg tctttctcgc ttaatggcac tgctgccaac
    20581 agacggtaac tttgcatgcg cagcgatccc gctttgttga tcgcatgggc gctgccctga
    20641 acgccttgca ccagccagcc agaaaccgcc atccctgcca gtccaatagc agtagaaagc
    20701 aacacaataa gcgcaacctg attaaccagg gtgagcggag agagacaacg tttaagcatg
    20761 taaacctctt ccttcaggct ttaaatgagc aataacctta atgaatgtga cgatacattc
    20821 tggaatggca gtattctcgg ctattggctg aagtataccc atacccggaa agagttactc
    20881 cttatttgcc gtgtggttag tagctttaca tcggtaaggg tagggatttt acagcaccgt
    20941 gaaaaatctc ataattttta tgaaatcact gtactcacta tgggtaatga taaatatcaa
    21001 tgatagataa agttatctta tcgtttgatt tacatcaaat tgcctttagc tacagacact
    21061 aaggtggcag acatcgaaac gagtatcaga ggtgtctatg agtcactcat ccgcccccga
    21121 aagggctact ggagctgtca ttacagattg gcgaccggaa gatcctgcgt tctggcaaca
    21181 acgcggtcaa cgtattgcca gccgcaacct gtggatttcc gttccctgtc tgctgctggc
    21241 gttttgcgta tggatgttgt tcagcgctgt tgcggtgaac ttaccgaaag tcggttttaa
    21301 ttttacgacc gatcagctat ttatgttgac tgcgttgcct tcggtttctg gcgcgttatt
    21361 acgtgttcca tactccttta tggttcctat cttcggtggt cgtcgctgga cggcgttcag
    21421 caccggtatt ctgattattc cttgtgtctg gctgggtttt gccgtgcagg atacctccac
    21481 gccttatagc gtcttcatca tcatctctct gctgtgcggc tttgctggcg cgaacttcgc
    21541 atccagtatg gcaaacatca gcttcttctt tccgaaacag aagcagggtg gcgcgctggg
    21601 cctgaatggt ggtctgggca acatgggcgt tagcgttatg cagttggttg ctccgctggt
    21661 ggtatcactg tcgattttcg cagtatttgg tagccagggc gtcaaacagc cggatgggac
    21721 tgagctgtat ctggcgaatg cgtcctggat atgggtgccg ttccttgcca tcttcaccat
    21781 tgcggcgtgg tttggcatga acgatcttgc tacctcgaaa gcctccatca aggagcagtt
    21841 gccggtactc aaacggggtc atctgtggat tatgagcctg ctgtatctgg caaccttcgg
    21901 ttccttcatc ggcttctccg cgggctttgc gatgctgtca aaaacgcagt tcccggatgt
    21961 tcagattctg caatacgctt tcttcgggcc gtttattggt gcgctggcgc gttctgcagg
    22021 tggtgcatta tctgaccgtc tgggcggaac tcgtgtcacg ctggtgaact ttattctgat
    22081 ggcgattttc agcggcctgc tgttcctgac cttaccgact gacgggcagg gcggaagctt
    22141 catggcgttc ttcgcggtct tcctggcgct gttcctgaca gctgggctgg gtagtggttc
    22201 cactttccag atgatttccg tgatcttccg taaactgaca atggatcgcg tgaaagcaga
    22261 agggggttct gacgaacgtg cgatgcgtga agcggcaacc gacacggcgg cggcgttggg
    22321 tttcatctct gcgattggcg cgattggtgg cttctttatc ccgaaagcgt tcggtagctc
    22381 gctggcatta acgggttcgc cagtcggcgc aatgaaagta tttttgattt tctatatcgc
    22441 ctgcgtagtg attacctggg cggtatatgg tcggcattct aaaaaataaa tcttaatatt
    22501 tgattatcct ttgcgcggca tgatgtcgcg ctttttt
//
LOCUS       DBFIFS010000055        21608 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.055, whole genome shotgun sequence.
ACCESSION   DBFIFS010000055 DBFIFS010000000
VERSION     DBFIFS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.055"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..771)
                     /locus_tag="N7A07_001953"
     CDS             complement(<1..771)
                     /locus_tag="N7A07_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2476625.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     LWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKF"
     gene            complement(840..1265)
                     /locus_tag="N7A07_001954"
     CDS             complement(840..1265)
                     /locus_tag="N7A07_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2476626.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1249..1572)
                     /locus_tag="N7A07_001955"
     CDS             complement(1249..1572)
                     /locus_tag="N7A07_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2476627.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            1697..2173
                     /gene="racR"
                     /locus_tag="N7A07_001956"
                     /gene_synonym="rarC"
     CDS             1697..2173
                     /gene="racR"
                     /locus_tag="N7A07_001956"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HGU2476628.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            2489..2641
                     /locus_tag="N7A07_001957"
     CDS             2489..2641
                     /locus_tag="N7A07_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2476629.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(2756..3271)
                     /locus_tag="N7A07_001958"
     CDS             complement(2756..3271)
                     /locus_tag="N7A07_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476630.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            3395..3793
                     /locus_tag="N7A07_001959"
                     /pseudo
     CDS             3395..3793
                     /locus_tag="N7A07_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            3855..4124
                     /locus_tag="N7A07_001960"
     CDS             3855..4124
                     /locus_tag="N7A07_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2476631.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            4093..5211
                     /locus_tag="N7A07_001961"
     CDS             4093..5211
                     /locus_tag="N7A07_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2476632.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            5378..6172
                     /gene="potC"
                     /locus_tag="N7A07_001962"
     CDS             5378..6172
                     /gene="potC"
                     /locus_tag="N7A07_001962"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU2476633.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            6169..7215
                     /gene="potD"
                     /locus_tag="N7A07_001963"
     CDS             6169..7215
                     /gene="potD"
                     /locus_tag="N7A07_001963"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU2476634.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(7371..8192)
                     /gene="cobB"
                     /locus_tag="N7A07_001964"
     CDS             complement(7371..8192)
                     /gene="cobB"
                     /locus_tag="N7A07_001964"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU2476635.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(8208..9119)
                     /gene="nagK"
                     /locus_tag="N7A07_001965"
     CDS             complement(8208..9119)
                     /gene="nagK"
                     /locus_tag="N7A07_001965"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU2476636.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(9148..10392)
                     /gene="lolE"
                     /locus_tag="N7A07_001966"
     CDS             complement(9148..10392)
                     /gene="lolE"
                     /locus_tag="N7A07_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU2476637.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(10392..11093)
                     /gene="lolD"
                     /locus_tag="N7A07_001967"
     CDS             complement(10392..11093)
                     /gene="lolD"
                     /locus_tag="N7A07_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU2476638.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(11086..12285)
                     /gene="lolC"
                     /locus_tag="N7A07_001968"
     CDS             complement(11086..12285)
                     /gene="lolC"
                     /locus_tag="N7A07_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU2476639.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            12547..13620
                     /gene="ycfT"
                     /locus_tag="N7A07_001969"
     CDS             12547..13620
                     /gene="ycfT"
                     /locus_tag="N7A07_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU2476640.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            13748..17194
                     /gene="mfd"
                     /locus_tag="N7A07_001970"
     CDS             13748..17194
                     /gene="mfd"
                     /locus_tag="N7A07_001970"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU2476641.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            17338..18300
                     /gene="ldtC"
                     /locus_tag="N7A07_001971"
     CDS             17338..18300
                     /gene="ldtC"
                     /locus_tag="N7A07_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU2476642.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(18383..18640)
                     /gene="bhsA"
                     /locus_tag="N7A07_001972"
                     /gene_synonym="ycfR"
     CDS             complement(18383..18640)
                     /gene="bhsA"
                     /locus_tag="N7A07_001972"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU2476643.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18881..19513
                     /gene="comR"
                     /locus_tag="N7A07_001973"
     CDS             18881..19513
                     /gene="comR"
                     /locus_tag="N7A07_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU2476644.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(19575..20114)
                     /gene="ycfJ"
                     /locus_tag="N7A07_001974"
     CDS             complement(19575..20114)
                     /gene="ycfJ"
                     /locus_tag="N7A07_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU2476645.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(20341..>21608)
                     /gene="ndh"
                     /locus_tag="N7A07_001975"
     CDS             complement(20341..>21608)
                     /gene="ndh"
                     /locus_tag="N7A07_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU2476646.1"
                     /translation="GAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATG
                     SLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDEKGELLVPERKIAY
                     DTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGKVNI
                     AIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAA
                     AHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLE
                     TNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNNILA
                     QMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYRMHQ
                     IALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         5448 a         5616 c         5350 g         5194 t
ORIGIN      
        1 aaatttctgc tcccactgaa cctgcgtgaa aaccttcccc tccgccatcc agtaatcccg
       61 gaatgcggca agctcagcag gtgtaaactc aggctcaggc agagccatac cccacaatgc
      121 agcccgctgt cgaaaatcca gcgacggccg ccagacacca gtcatcggaa atttcccgat
      181 cggttcgctc aggccatccg ggtattcagg ttctgctgtc tgcaacggcg cgccattcga
      241 ctcactggct ggagcactct cgcgcatgcg cgcgttatgt gtggggttta attctttatc
      301 tgtatctgta tctgtcgtga cttgtcgtga catatgcgtg acgcgtcgtg actcatcgtg
      361 acaatcagtg ttctgctccc gcagtctttc ccgctcccgc tgcgctctct tgcgctctgc
      421 cggggatttt gcggtttgcg aaacattacc gttatcctcc ttcatcacct ggcgtttttc
      481 ccatccggaa ataagatcgc catccagaac tcgcccctgc attgcatgca aaattgaatc
      541 aatcacgtct tccgtcacat caagcgcact tgctaaatct tccgtcgtga catcaatgtg
      601 accacgtagt gactcgccgt gacatttcgt gacatttcgt gacgcactca ccagaaggtg
      661 gatatacacc gccatcactg ttgcgattgg ctgtcctgag accctggcaa tggttcgcca
      721 tttgggatca tttggcatgt catgccacaa tctgagccag gcattagcca tactcacctc
      781 atctgatacc gaactttacc ctcgaacatc cggaagaaat ccggcatgaa tattgttggt
      841 caatgcacga caacagcatt accaggctga cccccactgt tagtcagggt gccccaggcg
      901 atcgccgctg cgacaaaatc atccacatct ttcaccagcc gatccctccg ttcgacgatc
      961 tcacggtaat attcagagct gtgactgcgc atacgggcca ccagcagagg cggcattgcc
     1021 ttttcgatcg ccggtaacag agcctgcatt ttttcaacag catcaggggt gtctttatcc
     1081 agccaacgga aaattttctg ggtattacga gccagggctt ccggatggct gtcgtcgtac
     1141 agttccggga acgtcatccc cagttcgaaa taagtccggg ctatttcagc tgcaggaact
     1201 ttctcaccat caggatatgc ccaggcattc atcgccatgc ggatgtgctc atgtttgatt
     1261 ttcatgaatc atttgcctct tgatgcttcg ggtatgatcg ttttcgtcat ttggttgctt
     1321 catcgacata ttctgcgaat aacatgacga gcgtcgtaag tatgtccaat caacatcagg
     1381 acgaagttct tcacacagga caccaccttt tgttgctcgt tcaatcgcag gacatctctc
     1441 agcaggcaac tgacgtacac ctttgatcca ttgatttacg cttggaggag atacacctaa
     1501 aagcctagcc attgctgatt gcccaccgac aacagcacaa gctcgtttga atgaatagtt
     1561 atcttttttc atcgaatgaa ctccaaaaaa cacacaataa tattaggctt agcctaatgc
     1621 aactgtcaat aggctatgcc taacgcttcg agagtaggta ttgcctaatg cgatgcgcat
     1681 aggagactat taagcaatgc ttagtggtaa agacttaggc cgagcgatag agcaggccat
     1741 taacaaaaaa attgcatcag gagccgtcaa atcaaaggcg gaaatcgcac gtcatttcaa
     1801 agtccaacca ccatcaatcc atgactggat taagaaaggt tcgataagta aagacaaact
     1861 tccagaacta tggcgtttct tttctgatgt ggttggtcca gagcattggg ggcttaacga
     1921 ataccccata ccaaccccat ccacttcaga tacaaaaagt gaacttttag acataaacag
     1981 cctttatcaa gccgcctctg atgaaaaaag agcaattgtg gctttcctct tatctggaaa
     2041 tgctacggag cctagttggg ttgatcatga cgttcgtgcc tacattgccg caatggaaat
     2101 gaaggtagct aactatctga aaaatcaaga atcaaaacgg aaaagccaga acatcaccaa
     2161 gacaggaact taaacttata tggtccgacg ggaaattcct agttcccgtt agttaactcc
     2221 tactacctct cccacaaacc atcacctatt aggtcgcacc caaatcatta ggcgtagcct
     2281 attgacaagc aattaggcat ttcctatagt tttcccatac caacccatcc cgtcccacac
     2341 aatacagggc aatacctaga gttacccggc agtggtcagg gattaagtag ccagcccgag
     2401 gcgtaagaac atgacggcag ggttcaactt taactatgca gcaggttttt gttccgctac
     2461 cccggcgtta aggggatatg aggtcagcat ggatactctt gatcttggca acaacgaatc
     2521 tctggtgtgc ggcgtgtttc ccaaccagga cggcacgttc accgcgatga cgtataccaa
     2581 aagcaaaacg tttaaaaccg aatctggcgc gcgtcgctgg ttagcaagaa actctgacta
     2641 atgaaatctg gtaattaagg aatcctccac ggggggagag tggtgcacac gcgccggaca
     2701 caagcaagca tccggcatgc tctttaacaa tctggatatc cataacagta aaaatctaca
     2761 gattgccgtt cagttttctg gccaactctt caatagatgg aggtgatacg taatccggat
     2821 ttttattcat aagaaactga ttttcacagt acaggcacct gcttttatga aaaaattcat
     2881 attctctaac cgggaatggc tgaagtatcg atactatctt ttgtccaaaa cattttggac
     2941 aaagatgcat gattatgctg ccgccgttca cggttacctc cttcgagtac acaaaagttc
     3001 ccgactcaag ttggctaagg atataacctt ccgtctgagc ctcaaagttt tcaaattctg
     3061 caattttagc tttgagagaa gcatttattt cttgataagt gcccaccagc tcaacgagag
     3121 acacgcattc gcgctgaata gatgcaagct ttgagttcag ttcaccaata gccgcattta
     3181 cttcagcttg agtttttgcc tcgttcatta gttttgcaat ctgggcggtt tcacgaatag
     3241 ccgtcattgc tgccgttaat tcagcgatca cattcaatac tcttattgtt gttggggata
     3301 tccagattaa caagatcctt gttgttgggg aataacgggg accacctcgc ctgacgtggt
     3361 aaaaagcagg cacacaacac gaaagcacac ggcgagggac accattaaat ggtggcttaa
     3421 gcaatcacgc gaagcgcaat ctgccattat gaccgatgaa atcccgttag atgatgcact
     3481 gttacaattg cgggaattta tcgacgaaaa ctccggtgaa ttttttgttc aggtctgggg
     3541 aaatggagcc aacttcgaca acacgatttt gcgccgttca tacgaacggc aggggatccc
     3601 ctgcccgtgg cgttactaca acgatcgcga tgtacgcaca atcgttgagc tggggaaagc
     3661 catagacttc gatgccagaa cggctattcc attcgaaggt gagcgccata atgcacttga
     3721 tgacgcccgt taccaggcaa aatacgtttc agttatctgg caaaaactga tcccgagtca
     3781 ggctgatttt taatgttcaa ccgtcgccag ttgtcgttga tattctgcaa ctggcgcgtt
     3841 ccggagtgat agccatgagc gaacagtacc tgataacgct cgacgagtgg aaaccaaaac
     3901 ggttcagtct cccaataaca aacactaccc tggtgaaata cggaaaacta ggatacatcg
     3961 ttccaagacc acaaaaaatt cgtgggcgtt ggctgataga tcgccgagca gtatttgttg
     4021 ggcctggtga aacgggaatt gcgccggaaa ttcatactgg cgatgatgat gcactgaagg
     4081 agattttaac tcatgtcacc gaggccacga aaaaacagca ctgacgtagc cggtctttac
     4141 gaaaagtttg atcgcagaac tggcagagtt tactaccagt ataaaaatcc tgtgactgga
     4201 aaatttcacg gactcggaac agacaaaggt aaggcagaaa aaatcgcttc cacagccaat
     4261 cagcgaatag ctgcagcaga agctgaatat ttcatgcgca aaattgatga aagtccgtca
     4321 gcaacaaaac gtcggggtat cagattaaag gcatgggttg atcgatatct gaaaatacag
     4381 gacacgcgac tgaaaaatgg agatattgca gctacaactc acaaagaaaa aactcgaatg
     4441 gctgcatacc tggtttcccg tctgggaaac cacccattga aagaactgga agtaagagac
     4501 tttgcattaa tactggatga gtggctggat aaagacatgg tcagtacagc gagagtaaat
     4561 cgtggattat gggttgatat ttataaagaa gcacagcatg caggggaagt tcctcctgga
     4621 tggaatcctc cggaggctac ccgtaaaccg atccctaaag taaccagagc caggctcacc
     4681 atggaagact ggcaaaaaat ttacaatgca acgcctgaaa aacactttat ccgtaacgca
     4741 atgcttcttg cgattgttac tggtcagcgc cgtgatgaca tttgccacat gcgtttttca
     4801 gatgtgtgga acgaacactt gcatatcacc cagggaaaaa ccggaatgcg tctggcgtta
     4861 ccgcttacac tacgctgtga tgccattggg ataacgttaa aagaagttat tgatgggtgc
     4921 cgagacagaa tattaagtcc atatctaatc catagtcggc accagaaaca accgaagccg
     4981 atgagtaaag acaacctgag cgactacttt gccaaagcac gggatctggc tggggtaatt
     5041 ccaccagcag gaaaaactcc gccaacattt catgaacaac gctctctatc agaacggctg
     5101 taccgtgcac agggtatcga tacaaaaaca ttactaggac ataaagtcca ggcaaccacc
     5161 gatcgctata acgatactcg aggtcaggaa tgggttaagt tggttatttg acaaacacca
     5221 tatggaatat ccgtcaccat ctcaggaaaa aagtgttgta aaatgcgtgg cctagttttg
     5281 gggaaacgtt ttggagagat tttggagaaa ggaaaaaatt gaataaattc aaaatcctga
     5341 aagtgtcccg ccttatgaat aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg
     5401 cggttttatg accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa
     5461 ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag
     5521 cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt
     5581 gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta
     5641 tcgctttcgt ggtaagccgt tcgttagcgg aatgctgttt gtggtgatga tgtcaccaga
     5701 tatcgtgatg gcgatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt
     5761 ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta
     5821 ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag
     5881 cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcagg
     5941 ctgggtgtta agctttaccc tgtcgatgga cgatgtggtg gtttcttcgt tcgtcaccgg
     6001 gccgagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc
     6061 ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag
     6121 ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg
     6181 tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat
     6241 gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa
     6301 cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc
     6361 atgtatgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc
     6421 tattacgtcg ataaaatgcg taaagaaggg atgatccaga agatcgacaa gtcgaagtta
     6481 acgaacttca gcaatctcga tccagacatg ctcaataagc cgtttgaccc gaataacgat
     6541 tactccattc cgtatatctg gggtgcgacg gcgattggcg ttaacggtga tgcggtggat
     6601 ccgaaatctg tcaccagctg ggccgatttg tggaagcctg agtacaaagg cagcctgctg
     6661 ctgaccgacg atgcccgtga agtgttccag atggcgctac gtaagctggg ctactccggt
     6721 aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca
     6781 aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac
     6841 ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac
     6901 gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat
     6961 gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca
     7021 aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta
     7081 agtccagaag tggcgaacga taagacgctc tccccggatg ctgaaaccat taaaaatggc
     7141 gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg
     7201 aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca
     7261 aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg
     7321 gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc
     7381 ttcagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg
     7441 gcaaattcat taccgacctg acttggttca agattcagct ccacggtgtg cgcgccatgc
     7501 agtttcgctt cgtgaacaaa cccagccgcc ggataaacat gcccggaagt accaatggca
     7561 atgaaaatat cggccatcga caacgccata taaatttcat ccatgccgag tggcatttcg
     7621 ccaaaccata ctacgtgtgg gcgcaagggg gccgggaact ggcagcaatg gcatttatct
     7681 tctggggtaa cgtctccggt ccagtcgaga acctgaccac tttgtgaaca acgaactttc
     7741 agcagttccc catgcatatg aatcacattg gtattacctg cgcgttcatg caggttgtct
     7801 atattctgcg tcaccagcaa aaagcgatcg ccgagagcat cttgcagttt agccagcgca
     7861 agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa
     7921 aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc
     7981 cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa
     8041 attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta
     8101 tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt tttacgaaaa
     8161 cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga
     8221 tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc
     8281 ggaacacgag ctacaggtaa gagatgacga ggcagcctgt ccgccagttg cgttgtgatt
     8341 gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg
     8401 atatttccca gacaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc
     8461 tcatcgcctt gatcataaag cgcaataatt tcgggagccg gcaacggttg atgataatag
     8521 tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga
     8581 ccacagccgc agcggcgtaa cgggaaatcc agccccatca tggttaacgc atcaaccggc
     8641 agacgcatat ggccaaactc gccggtaatg tagcttttcc cggtaatcgg tttgccgttg
     8701 aaaatcagcc cgccgccaac gccggtgccg agaatcaacc ccatcaccaa cggatattgc
     8761 gtaaattcgt catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt
     8821 acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct ggcagcaggg
     8881 acattggcgg catacagcgt accatcttct gtttccggca ttcccggaat accgatgccg
     8941 acagagcctt tacagccaaa acgttgatca gcttcggcta ccagctcgca cactgcatct
     9001 aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga
     9061 ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt
     9121 gccgctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat
     9181 attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac
     9241 cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaatagat
     9301 atcgctggag aggaactgat gaccgatcag cttttcaatc cgctcaataa tcggggtaag
     9361 ttgcagtgaa acaactacgc cgataatcac accacacagg ctaccgaata gccctgccag
     9421 caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa
     9481 tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa
     9541 acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat
     9601 atcgcgatac atatagccgt aagtaccaat ccagctttta atataaacat agctgttggt
     9661 cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc
     9721 aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat
     9781 ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaacgt gcaaacgcac
     9841 acgttttggc tgcatcagtt tatgctcagg attcgagttg gggatcataa tcgacaccca
     9901 atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc
     9961 gcccgcttta aaattgcgcc aggcatcccc ctgaacaaac gagggtaatg cgctcagacg
    10021 ctgttcctgt tgcgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc
    10081 caccagcccg gtgaaattga tatacggcgc agcggcggca atacctggca ctttttgcac
    10141 gttatccagt gcttcctgcc agttagtcca cggttgatcc accgcctcta tttcgccatg
    10201 cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa
    10261 gccgacgatc aataccgcca cgccaagggc aatgccaatg gtagaaatga cggagatcag
    10321 cgacaccatg ccgccgcgtc gccgtccgcg gctaaaacgc aggccaatca ataacgataa
    10381 aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg accatcacgc
    10441 atctccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac caccaggaag
    10501 gcggtgccct gcaagcgatt caattcccca agcaactgga agatgctgtc tgcgttacgc
    10561 gcatcgaggt taccggtagg ttcatccgcc agtaccagcc acgggttatt gaccagcgca
    10621 cgggcaatcg ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca
    10681 cgatgctcca gccccaccgc ttttaacatc tcaagtgcac ggctgttgat ttcagcgggc
    10741 tttttcttgc caatcagcag cggcatagcc acgttttcca gggcagtaaa atccggcagc
    10801 aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgcc
    10861 gaagacagtt tgctcatcgg ttgaccgtta aagatcacat cgccggaggt tggtgtatcc
    10921 agcccgccca gcaggtgcag caaggtactt ttaccggaac cagagctacc gacgatcgcc
    10981 atcatttcac cttcgcccac gctgaaactg acattgtgca gtacatcggt ttgcacactg
    11041 ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa
    11101 agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc
    11161 gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag
    11221 cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag
    11281 cagtgcgcca agcgccgctc cgaggatcgc accgataatc ccggcgctgg ccccttgcac
    11341 cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgctacttc
    11401 gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacggc
    11461 gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag
    11521 ctcgccttta cgatcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact
    11581 taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa tattgcctgc
    11641 cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac
    11701 ttcactgtta gcggcaaaag taccaatcac attgaacagg cgctgacttg gaatacgccc
    11761 catcggcgtg aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattaac
    11821 gcctagctgt gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc
    11881 agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg gatcgatacc
    11941 cagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat
    12001 aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt
    12061 aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg
    12121 cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag
    12181 ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg ctgcacgccc
    12241 acgcatgtaa cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt
    12301 gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa
    12361 gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt
    12421 tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc
    12481 attacacctc gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa agagcgtgtt
    12541 gtcctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg
    12601 gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg
    12661 ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc accctttcgt
    12721 atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg
    12781 tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc
    12841 gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta
    12901 agcaatgcct ccaatgccgc gtatgccgat tccaccggtg agttcctgca cgggatgatc
    12961 accgccagca ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa
    13021 atttttagcc gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt
    13081 aatttcgttc ccacgccgtg gtggggaatg aacagtgtga tccgcaattt gccttattac
    13141 agccttggtg catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc
    13201 cgccatctgc tgatggcttc tttgctggct gctctggcgg tcggtgcctg gttgtttact
    13261 atctcgctgc tgttgtcgct ggtgtcgatt gtggtgatca tgaagttgtt ttatcagtat
    13321 gaacaacgtt ttggcatgcg ttccaccagt ctgctgaatg tgattggctc caacactatt
    13381 gctatctaca ctacccatag aattctggta gagatattca gcttaactct gcttgcgcaa
    13441 atgaacgcag cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttaccccttt
    13501 gttagtttgt tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgagca
    13561 ttcagcgatc tgttgttctc cccgccttca ctgcccgttg ccgtcagtta ctcccgctaa
    13621 gccatcgccc cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac
    13681 agcattgctt atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac
    13741 aaccgttatg cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct
    13801 gctgggcgag ttaaccggcg ctgcctgcgc gacgctggta gcggaaattg ccgaacgtca
    13861 cgccggaccg gtggtgctta ttgcgccaga tatgcaaaat gccctgcgtt tgcatgatga
    13921 aattagccag tttaccgacc agatggtgat gaatctggcg gactgggaaa ccctccctta
    13981 cgacagtttt tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct
    14041 accgacaatg cagcgtggcg tactgattgt tccggtgaat acacttatgc agcgcgtttg
    14101 cccgcacagt tttctccacg gtcatgcgct ggtgatggaa aaaggtcagc gcctgtcacg
    14161 agatgcatta cgaacccaac tggatagcgc cggttatcgc catgttgacc aggtgatgga
    14221 gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagtt
    14281 gccttatcgt cttgatttct ttgatgatga aatcgacagt ctgcgggtgt ttgacgtcga
    14341 cagtcagcgc acgctggagg aagtagaagc gatcaacctg ctgcctgcgc acgaatttcc
    14401 gaccgataaa gcggcgattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa
    14461 gcgcgatccg gaacatattt accagcaagt gagtaaaggc acattacctg ccgggatcga
    14521 gtactggcag ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc
    14581 caataccttg ctggtgaata ctggcgatct ggaaaacagt gccgaacgtt tccaggctga
    14641 cacgctggcg cgttttgaga atcgcggcgt cgatccgatg cgcccactgt tgccaccaca
    14701 atcgctctgg ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca
    14761 gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact
    14821 gccagacctg gccgttcagg cacaacaaaa agcgccgctg gatgcgctgc gtaagttcct
    14881 cgagtctttc gacggtccgg tggtgttctc ggtagaaagt gaaggccgcc gtgaagcgct
    14941 gggtgaactg ctcgcgcgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc
    15001 cagcgaccgt gggcgttatc tgatgattgg cgctgcagag catggttttg tcgatacggt
    15061 acgtaatctg gcgctgattt gcgaaagcga tctgctcggt gaacgcgttg cccgtcgtcg
    15121 tcaggattct cgccgcacca tcaaccccga tacactgatc cgtaacctcg cggaactgca
    15181 tattggtcag ccggtggtcc atctggagca cggcgtcggg cgctatgccg gaatgaccac
    15241 gctggaagca ggcggcatta ccggcgagta tctgatgctc acctatgcca acgacgccaa
    15301 attgtatgtt ccggtgtcgt cgctgcatct gattagccgt tacgcgggtg gtgcggaaga
    15361 aaatgccccg ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc
    15421 ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa
    15481 agagggcttc gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg acagcttccc
    15541 gtttgaaacc acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca
    15601 gccgctggca atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc
    15661 gatgcgcgcc gctttcctgg cagtagataa ccacaagcag gtggcggtgc tggtgcctac
    15721 cacccttctc gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt
    15781 acgtatcgaa atgctctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga
    15841 agtggcggaa gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt
    15901 caagtttaaa gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca
    15961 taaagagcgc attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc
    16021 gatcccacgt acgctgaata tggcaatgag cggaatgcgt gacctatcga ttatcgccac
    16081 gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt
    16141 ccgcgaggcg atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga
    16201 tgtggaaaac attcagaaag ccgccgaacg gctggcagaa ctggtgccag aagcgcggat
    16261 cgccatcggt cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca
    16321 tcatcaacgt ttcaacgtgc tggtttgtac aaccattatc gaaaccggga tcgacatccc
    16381 gacagccaac accattatca ttgaacgcgc ggatcacttc ggtctggcgc aactgcacca
    16441 gttacgcggt cgcgtcggac gttcgcatca tcaggcatat gcatggctgc tgacgccgca
    16501 tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga
    16561 tctcggggca ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact
    16621 gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt atatggagtt
    16681 gctggaaaac gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac
    16741 cagccagcaa acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcattcc
    16801 tgacgtgaat acgcgtctgt cgttctataa acgtattgcc agcgccaaaa cggaaaacga
    16861 actggaagag atcaaagtcg agcttatcga tcgcttcggc ctgctgccgg atccggcgcg
    16921 taccctgctg gatattgccc gtctacgcca gcaagcgcag aaactgggga tcaggaagct
    16981 ggaaggtaat gagaaaggcg gcgtgatcga atttgccgag aagaatcacg ttaatccggc
    17041 ctggttgatt ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg
    17101 cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt
    17161 tatgcgtgaa ctggaagaaa acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc
    17221 cccatctgca acatttacaa attctttgca cttccctgca ctatccgaca ctgtcaccat
    17281 ccataattca ggcttatctg tttattacaa taaccttata tttattatgg atttttggtg
    17341 atgatcaaaa cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg
    17401 ctggcgctac cggcaaaagc caacacctgg ccgctgccgc cagcgggcag tcgtctggtt
    17461 ggcgaaaaca aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa
    17521 aaatataacg tcggctttct cgctctatta caggctaacc ccggcgttga tccttacgta
    17581 ccgcgcgcgg gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc
    17641 gaaggcattg tgatcaacat tgcggagctt cgtctctatt actacccgcc gggtaaaaat
    17701 tcggtaaccg tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca
    17761 atggtgacca ccgtttctga caagcgtgca aacccaacct ggacgccaac ggcaaacatt
    17821 cgcgcgcggt ataaggcaca aggaattgag ttgcctgcgg tagtgccggc tggaccggat
    17881 aatccaatgg gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac
    17941 ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg
    18001 gatgacgata tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc
    18061 aacactccga taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag
    18121 ccgctgtcag agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca
    18181 atgcaatcat ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat
    18241 gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aacgctgtaa
    18301 atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc
    18361 aggcattaat gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg
    18421 gaccggttac agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca
    18481 gctgctcttc cagcgatccc agatttgtcc ccgcgttagc actgattgta ccgacttttt
    18541 gttggccttc tggcgttgac tgaacttcga cagccgcaaa gctggcaaat gacatggagc
    18601 ttaaaatcgc cgcagcgatg agggttttta cgtttttcat aatagtggcc ttatgcagat
    18661 gaatgacgtc gcaagcaatt acttaacgat cgataactaa atgatagatg tgatctggat
    18721 cacatacaag atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt
    18781 taaatagaaa gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta
    18841 taataacctt tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca
    18901 atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa
    18961 ggccatgaaa ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga
    19021 agcgaccgga gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt
    19081 tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt
    19141 ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg
    19201 ctttaccggc aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc
    19261 cccagattca ggagatatcg ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac
    19321 tttgcagcag tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt
    19381 gacacatctg gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga
    19441 aggtgcatcg ttggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga
    19501 actggtgaaa taagtcgcaa aaaaagcccc tcagcctgag cgaatgaggg gccaaattgc
    19561 agagtacagc cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg
    19621 atctgcgtac ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag
    19681 gtcacatcat aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc
    19741 gtagtcgtgt aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca
    19801 ccacccagcg cccccacaac agtggcgaca tctttaccac gaccaccacc aaactgatgc
    19861 cctatcacgc cgccagcaac agcgccgagc accgacccgg taatgcgatg ttcatcctgc
    19921 accggtcgac gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt
    19981 tccttgattg gggttgcaga aacaacctga gcgtattgcg ggccacgttc aaacacgttc
    20041 agactggcca ctgccgctac gccaagcgca gctgcgacac caatcccgat acccgccaac
    20101 attgatttat tcacgggaat acctccttct ttgcagttat gccaatcatg caagcgaagg
    20161 agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa tacttaagga
    20221 ttcggaggag tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg
    20281 ctcgtaccat ttgtccaggg cggatgaggc gtttatgcca catccgccag tgtacgtcga
    20341 ttaatgcaac ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa
    20401 tccggtttta aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac
    20461 aaagcgcgca attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct
    20521 accaacggtg gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt
    20581 tttcagcagc ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg
    20641 gtgtgcagcc tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca
    20701 gtcgccaata gcgtaaatgt ctggatcgcg ggtggtttgc agcgtcagtt ccaccaccag
    20761 ctggttgata cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat
    20821 cccggctgcc cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc
    20881 gccttcatca gcactggtga ccatggtttg cgtcagaacg cgaacgccaa gtttcgttag
    20941 ctcgttgtgg gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc
    21001 ttctaccagc gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag
    21061 ttgcttgacc gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac
    21121 aatcgcaatg ttcactttgc catttgcgcc caggttggcg gagtatttca ggaagagatt
    21181 cagcatctcc tggtgaaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc
    21241 tttgacacct ggtgtattga aatcgttaga ggtgctaccc agcgccatta ccagggtgtc
    21301 ataggcgatt ttacgttccg gaaccagcag ttcacctttc tcatcgcgca gttctgcaat
    21361 agtgattgtt ttcgcgtcac gatcaatatc aatgacggaa cccagctgga actggaaacc
    21421 atgattgcgc gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt
    21481 cgctacttcg tgcagcagcg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat
    21541 tttggctttt ttcttgcgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc
    21601 agcaccgc
//
LOCUS       DBFIFS010000056        21298 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.056, whole genome shotgun sequence.
ACCESSION   DBFIFS010000056 DBFIFS010000000
VERSION     DBFIFS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.056"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            12..1655
                     /gene="sapA"
                     /locus_tag="N7A07_001976"
     CDS             12..1655
                     /gene="sapA"
                     /locus_tag="N7A07_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU2476647.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            1652..2617
                     /gene="sapB"
                     /locus_tag="N7A07_001977"
     CDS             1652..2617
                     /gene="sapB"
                     /locus_tag="N7A07_001977"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU2476648.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            2604..3494
                     /gene="sapC"
                     /locus_tag="N7A07_001978"
     CDS             2604..3494
                     /gene="sapC"
                     /locus_tag="N7A07_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU2476649.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            3494..4486
                     /gene="sapD"
                     /locus_tag="N7A07_001979"
     CDS             3494..4486
                     /gene="sapD"
                     /locus_tag="N7A07_001979"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU2476650.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            4488..5294
                     /gene="sapF"
                     /locus_tag="N7A07_001980"
     CDS             4488..5294
                     /gene="sapF"
                     /locus_tag="N7A07_001980"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU2476651.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(5346..6509)
                     /locus_tag="N7A07_001981"
     CDS             complement(5346..6509)
                     /locus_tag="N7A07_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HGU2476652.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(6518..7891)
                     /locus_tag="N7A07_001982"
     CDS             complement(6518..7891)
                     /locus_tag="N7A07_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HGU2476653.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(7895..11003)
                     /locus_tag="N7A07_001983"
                     /pseudo
     CDS             complement(7895..11003)
                     /locus_tag="N7A07_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(11003..12124)
                     /locus_tag="N7A07_001984"
     CDS             complement(11003..12124)
                     /locus_tag="N7A07_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU2476654.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(12273..12839)
                     /locus_tag="N7A07_001985"
     CDS             complement(12273..12839)
                     /locus_tag="N7A07_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU2476655.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            13317..14105
                     /gene="fabI"
                     /locus_tag="N7A07_001986"
     CDS             13317..14105
                     /gene="fabI"
                     /locus_tag="N7A07_001986"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU2476656.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            14249..15376
                     /gene="yciW"
                     /locus_tag="N7A07_001987"
     CDS             14249..15376
                     /gene="yciW"
                     /locus_tag="N7A07_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU2476657.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            15444..17378
                     /gene="rnb"
                     /locus_tag="N7A07_001988"
     CDS             15444..17378
                     /gene="rnb"
                     /locus_tag="N7A07_001988"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU2476658.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(17497..17559)
                     /locus_tag="N7A07_001989"
                     /pseudo
     CDS             complement(17497..17559)
                     /locus_tag="N7A07_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17613..19593
                     /gene="pdeR"
                     /locus_tag="N7A07_001990"
                     /pseudo
     CDS             17613..19593
                     /gene="pdeR"
                     /locus_tag="N7A07_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(19575..19703)
                     /gene="ymiD"
                     /locus_tag="N7A07_001991"
     CDS             complement(19575..19703)
                     /gene="ymiD"
                     /locus_tag="N7A07_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU2476659.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            19741..19914
                     /gene="yciZ"
                     /locus_tag="N7A07_001992"
     CDS             19741..19914
                     /gene="yciZ"
                     /locus_tag="N7A07_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU2476660.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            20004..20753
                     /gene="yciT"
                     /locus_tag="N7A07_001993"
     CDS             20004..20753
                     /gene="yciT"
                     /locus_tag="N7A07_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU2476661.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            21022..21240
                     /gene="osmB"
                     /locus_tag="N7A07_001994"
     CDS             21022..21240
                     /gene="osmB"
                     /locus_tag="N7A07_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU2476662.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         5187 a         5547 c         5246 g         5318 t
ORIGIN      
        1 cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg
       61 gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg
      121 tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa
      181 ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct
      241 atcgcctgat gccagaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc
      301 gcttccacct gcgtcgcgat gtcccgtttc aaaaaaccgc ctggtttact cccactcgca
      361 aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc aacaacccgt
      421 ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt gccgataacg
      481 tgaaaagcgt ccgtaaactg gataatcata ccgttgagtt ccgtctggct cagccggatg
      541 cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc
      601 ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcactggac
      661 cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact
      721 tctggcgcgg taaaccgtta atgccgcagg tggtggtgga tttaggctcc ggcggcaccg
      781 gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct gctgccagcc
      841 agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct gggatgaacg
      901 tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg
      961 cgctggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg
     1021 aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta
     1081 ctgaatacaa tccggcgaaa tcgcgcgaac agttgaagtt gttggggctg gaaaatttaa
     1141 cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg
     1201 ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag
     1261 aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg ttatccggtt
     1321 gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc tgcgcggcaa
     1381 ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc gtgttgcgta
     1441 agacgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa gcgcagagta
     1501 ttctggcgca ggaattgccc attttgccgc tggcgtcgtc attgcgtttg caggcctatc
     1561 ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt
     1621 atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac gccgcatttt
     1681 gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga gctatttcac
     1741 tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaacgg
     1801 cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg ccgaacagtt
     1861 aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt ttgcgctgat
     1921 tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt
     1981 gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt
     2041 gacactgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct
     2101 ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc
     2161 atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct
     2221 gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta tcgaagtgta
     2281 tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg
     2341 tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc
     2401 caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg
     2461 ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt
     2521 gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa
     2581 ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc
     2641 cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc
     2701 tgtacggctg cgcggggctg gctgtactgt gtatgtttgg cggctggttt gcgccttacg
     2761 gcatcgacca gcaatttctc ggttaccaat tattgccgcc atcatggtcg cgctatggcg
     2821 aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc cgtttactga
     2881 gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac acttgccgcg acgatctgtg
     2941 gcctggtact gggcaccttc gccggggcga cgcacggcct ccgctcagcg gtgcttaacc
     3001 atattctgga taccttactg gcgatccctt ccctgctgtt ggcaattatc gttgtagcgt
     3061 ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcgctg ctgccgcgta
     3121 tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg
     3181 cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg cctaacatca
     3241 ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg
     3301 cgctgggctt tctcgatctt ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg
     3361 gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa
     3421 ttatgattag cgtgctgttg gttaacctcc ttggcgacgg tgtccgtcgt gcaattattg
     3481 cgggggtgga ataatgccat tactcgatat tcgtaacctg actattgaat ttaaaaccgg
     3541 tgatgagtgg gttaaagccg ttgaccgggt aagtatgacc ttaaccgaag gtgaaatccg
     3601 cggtcttgtt ggtgaatccg gttccggcaa aagtttgatt gcgaaagcga tttgtggggt
     3661 gaataaagat aactggcgtg ttactgcaga ccgtatgcgt tttgatgata tcgatttgct
     3721 gcgtctctcc gcacgcgaac ggcgcaaact ggttggccac aacgtgtcga tgattttcca
     3781 ggaaccgcag tcgtgtctag atccttcaga acgtgtgggt cgccagttga tgcaaaacat
     3841 cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc
     3901 cattgaactg ctgcaccgcg tgggaattaa agatcataaa gatgcgatgc gcagttttcc
     3961 ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca
     4021 accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa cccaggcgca
     4081 aatctttcgc ctgctgacgc gtctaaacca aaacagcaat accactattt tgcttatcag
     4141 ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca
     4201 aacggtggaa accgcgccga gtaaggagtt agtgacgatg ccacatcatc cttataccca
     4261 ggcgctgatc cgcgcgatac cagacttcgg aagcgcgatg ccgcataaaa gtcgcctcaa
     4321 tacgctaccc ggcgctatcc cactgcttga acagttaccg attggttgtc gtctggggcc
     4381 acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa
     4441 tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc
     4501 tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg tttcgtcgtc
     4561 agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga
     4621 ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg ggaatgatag
     4681 aacctaccag cggcgagtta ttgattgacg atcatccact gcattttggc gattattcct
     4741 tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc
     4801 aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccaacctg gaaccggaac
     4861 agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca
     4921 gttactatcc gcatatgttg gcccccgggc aaaaacagcg tctgggtctg gcgcgcgcgc
     4981 tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg gatatgtcga
     5041 tgcgttcgca gttgattaat ctgatgctgg aattacagga aaaacagggc atttcgtata
     5101 tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc
     5161 atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg
     5221 aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacggcg gatgcgtggc
     5281 gtaaggaccg ttagtgttct gcacctcatc ctgacagggg aacccccctc aggatgaggt
     5341 attcattact tttgattgtc gattctggtg agagcaacca ggaatgccag tgtataacac
     5401 accgtaattg ccagactcat ttttaacagt gtttctccgc caaatcccga caatggcgcg
     5461 gctataccgc cgaagacaaa catgctcatc cccatcaacg cagacgccgt tccagattcc
     5521 tgcgtcccta ctgcactcat agcctctgcc ccgccaacag tgccgatgcc gctacaaaat
     5581 gcaatagtga aaaataatgc caccagtgcc ggaatgggca attgtgtcca tgcgcataag
     5641 accgtgagca atgcacacaa aattgccgct atcaggccac ctcgcaacaa tgtcatcgca
     5701 ttaatccgtc gcgccagacg cgaaaagatc catgaagcaa tgataagtcc gatgccgtta
     5761 aggccaaaca ccaggctaaa ttgcattgga ctaaagccaa attccttctg caacacgaac
     5821 gacgaagagc cgatatacgc aaacaagcct gccagcataa aactttgaat caggcaaaag
     5881 cgcataaaac ggcggttctg caccacgctt cgtccggtta atagcaatga tgagcccctt
     5941 ttattttctg gcaaggtctc attaataaat aacacgcagc ccagcaacaa tacggtgcta
     6001 atttcagcca ttacccagaa taaagtgcgc cagtcaaaag tgctaacaat gtacccgccc
     6061 agcactggcg acaacaccgg tgccaggcca ttcaccgtca ttaacagcgc aaaaaactgg
     6121 gtcaacgtta ctccctgata tttgtcacga gcaatagaac gagagagcac cgaaccaccc
     6181 gcccccgcga tcccttgaat aaaacgccag accaccagcc agtaaatatt gttcgttgtc
     6241 gcgcacaaaa tggaagaaac aataaacaat agcaacgaca agattaacgg acgtttacgc
     6301 cctattttgt cgctcagagg gccaaataac agttgtccga cgccaagacc gatcagtgat
     6361 gcagtcagcg ttaattgcgt tatggtcgtg gttgccgcca gttgctcaga catctccggc
     6421 aaagccggca aataaagatc ggtacacatc gggccgatac cggctaaaag accaagaatc
     6481 aatgcccatg aaagagagac tctagccata ttattcctta cttgagtgat gacatgccgc
     6541 cgcccagcga ctgccagagc gtgattcggt ttgtaaaatc agtcagttgc agtgccagca
     6601 gctcctgctg tgcgctccac aagctgcgct gcgccgtcaa cacggtcagg taatcaccaa
     6661 cacccgtctg atagcgacgc aagccaacat cgaccgtttg ttgctcagct ttaacatact
     6721 ggcgctgtgc atccagttgc tcttcaagcg tggtccgccg cgccagtgcg ttagcgacat
     6781 ctttaaatgc tctctgaacg gttttttcat aggtggcaat gagtccacgc ttttgtgctt
     6841 ctgcgtaacg aagctgcgcc agattacttc cgccagtgaa cagcggtaat gtgaccgatg
     6901 gcgtaaatga ccagatctgc attccgtggc tgaacaacga cgacaatgcg tcactgccaa
     6961 caccagcgct ggcagtcagc gaaatcgtcg ggaaaaagtt agcccgcgcc gcgccaatat
     7021 cggcattggc gctcttcaaa ttatgttccg cttcctggat atccgggcga cgcagcagaa
     7081 cgtcagatga cactcccgca ggaaccagac ttatcatctg ttccggcagg ctttccagtg
     7141 ttcccggtag caaattttcc tcaagcgttg tacctgccag taagttcagc gcatttttat
     7201 cctgcatcac ttgtgtctga tagctggcaa cgctggcgcg cgcttgctga taaacactca
     7261 tcgcctcact gacatctgtc gctgccgccg tgccaacctg ttgctggcgc tggataattt
     7321 tcaatgagtt ttctgcactg gtcatcgttt cttttgccag ggccaggttg ctgttatcgg
     7381 ccgccagtgt cagccaggcg gtgctgattt cagcaatcag cgttaaacgg gtgttctggg
     7441 ctgtaaattc actggcaagc caggtttctc gcgccgcccg agataaactc tgattacgac
     7501 caaacagatc taacgtaaaa ctggacaccg tgccgtcagc ctcggcagtg gtttccgtac
     7561 cattcgccag cgaacgactg cgggttgaac ttaaggccgc attcaccgtc gggaataacg
     7621 acgcattagt ttgcccatag agcgcccgtg ctgagtcaat atcagcaatc gctttttgca
     7681 catcgcggtt actgttcagt gcaatagtca ccacctgctg caatcgggga tcgtggatca
     7741 cctgctgcca gtcatggctg attgcctttc cctggccctg tgctcccggc aaggtcgccg
     7801 ggattgggga ttcaggcgtg ctgtagtggg gatcgagcga gacacatccc gcactcaaca
     7861 gcaccagaaa aataagagaa cgacgcaaca taacttactc ctgacggcgc gatttaccgg
     7921 caaacaaacg cttcaccagt acaaaaaaca gaggaacaaa gaaaatagcc agcaacgtag
     7981 cggtcagcgt accgccaata atgcccgtac caatggcgat gcggctgttc gcccctgagc
     8041 cggttgctat cgccagcggc attacccccc gcaataaacg ccagcgaggt catgatgatt
     8101 gggcgtaaac gagtctgcgc cgcgcgtaat gccgcacgac tcaaggaata accctctgca
     8161 accgccgctt cagcaaattc cacaatcaga atggcgtttt tcgacgacaa gccgatagtg
     8221 gttaatagcg ccacctggaa gtaaacatcg ttgtttaaat cacgcatcca ggccgccagc
     8281 gccgcgccaa gaagccccag agggatcacc agaatgaccg agaacgggac tgaccagctt
     8341 tcgtacagtg ctgcaaggca gaggaaaacc accagaatag aaactgcata cagactcaat
     8401 gcctgaccgc tggccagttt ctcctgcaat gacaaaccac tccaggccca ggttgttccc
     8461 gcaggaagac tgtttgccag ttcctccatt tttgtcattg catcgccgga actaaagcca
     8521 gtcgcatttt ccccctgaat ttcataggct gcagaaccgt tgtagcgcac cagtctttcc
     8581 ggtccataca gccagcgggt ggtcgcaaaa gcagagaatg gtgtcatggc gttatcacta
     8641 ccgcgcacaa accatttacc taagtctgac ggcgcggaac gaaattcact gtcgccctga
     8701 atgtagactt ttttcacacg accacggtca ataaagtcat tcacataagt accgccccac
     8761 gcactggaca gggtgtcggt gacatcattc aaactcaacc ctaatgacac cgctttatta
     8821 ctatcaatat ctacctgcaa ttgcggcatt tgtggcaaat cattggcacg aacagaatgt
     8881 aactccggac tttgattcgc tttttctatc aactgattac gcatttgcag tagtgtttcg
     8941 cgatcggttc caccgttagc taacaattca aacgtaaaac cattgctttg ccccagccca
     9001 tcaacggctg gcggcgtcat cgcaaatacc gtggcatcac gaattgtgcc cagctctttg
     9061 gttgcccgta gggcgatagc ctgggcggtg ttttctgccc ctttacgttg agaccagttt
     9121 ttcaacgaaa caaacgccat cccggtgttc tgtccgctgc cgctaaaact gaaaccatca
     9181 acggtaaaaa tgacatcggt atttgctttc tcgttaatca ggaaccagtc aacaatctgg
     9241 cgattcactt ctgctgtacg ggcagcggtc gccccggccg gcagcgtgta ctgcaccatg
     9301 atttcgccct ggtcttcagt gggtaagaaa ctgcccggca gtttccacat catcagggcc
     9361 attccaccac ccagtacgag ataaagcccc atcgttcgag cagcacggcg caggacataa
     9421 attacgcctc gctgatattt atcttcagta cggcggtaaa agcggtcaaa tgcgccgaaa
     9481 aagccttttt tatgtggcgg aacatgctgg aggacggaac cgcacagggc gggagtcaag
     9541 gtcaatgcca ccaccacgga aagcagcatt gcggagataa tggttatcga aaactgacga
     9601 taaattaccc ccgtagatcc cccaaagaag gccataggca ggaatacggc tgacaacacc
     9661 agcgcaatgg caaccagtgc gccagaaatc tcgcccattg atttttccgt ggcttcacgc
     9721 gcgggcaacc ctttgtcacg cataatgcgt tcgacgttct ccactaccac aatggcatcg
     9781 tccaccagca agccgatcgc cagcaccata gcaaacaacg tcagggtatt aatcgaatag
     9841 ccaaacaacg caagaacgcc gaatgtgccc agcaaaacga cggggacagc taacgccgga
     9901 atcagtgtgg cacgcaggtt ttgcaggaat aaatacatca cgcaaaccac caatacgatg
     9961 gcttcaaaca gcgtctgaat tacgtcctct acagagattt taataaattc agtgctgtct
    10021 ttcgggtaag cgatgtcgta tccctgcggc atgttccgct ggaattcggc aatcttatcc
    10081 ttgaccagcg tcgccgtatt cagcgcattc gcaccgggcg aaagcattac agcaaccccg
    10141 gcagacgggt gaccgttaag tttgccgata gcggtataat cttcactgcc catctcaacg
    10201 cgagccacat cttttatacg aacaactgcg ctgtctgact ggcttttcac gataatattt
    10261 ttgaactgat ccaccgtctg caagcgagac tgcgcacgaa ccgttgcggt cagttgctga
    10321 gtattcggtg aaggcaatgc ccctattttc ccggcagtaa cctgcacgtt ttgcgcttca
    10381 atagcacttt gcacgtctga aggcattagc gagtaagacg ccagtttggc cgggtcaagc
    10441 cagatgcgca tagcgtattc cgcgccaaag acttgcagac tccccacacc ttcaacacgc
    10501 gccagcgggt cctgaacgtt actaaccaac cagtcggcga tatccgaact ggaggctttg
    10561 tcagtggtgt catacacggc agcaatcagc aaaaagttgc tttgtgattt ctccaccgta
    10621 acacccgttt gctgcacttc gctgggtagg cgcgactccg cctgctgaat tttattctgc
    10681 acctgaacct gtgcagtatc tggatcggta ccttgttcaa aggtcacatt aatactgacc
    10741 gaaccatcag agctgctggt tgagctgaaa tagagtaaat tatcaagccc agtgagttgt
    10801 tgctcgatga cctgagtcac gctgttctcc agcgtttcgg cagaagcacc agtataagtg
    10861 gctgaaattt taatggtcgg tggcgcaacg tcaggatatt gcgcgacggg caatgtgcga
    10921 atagccagaa tccccgccaa cataatcaaa atggcgataa cccaggcaaa gaccgggcgg
    10981 cgaacaaaaa agcgcgaaaa catcaggcgt tgcctccatt agcctgaact tcaacagcct
    11041 tgaccgtctg ccctgaagtg actttggcag aaccttcaac aatcagtcgg tcgccgctgt
    11101 gcaggccgtt tagcaccagc catttatcac catatgtttc tcccgtttcg agcgttcgct
    11161 gctctacttt attgtcttta ttcaccacca gcgcagttgc attgccttta gcatcgcgcg
    11221 tgacgccctg ttgcggcgcg agaatagcgt cttccatcac gccttcatcg acacgagcgc
    11281 gaacaaacat tcccggtaat aattgctgtt gtggattggg gaaaatcgcc cgtaatgtca
    11341 ccgaaccggt agactcatct acagcgactt cggtgagttc cagacgccct ttttcgctgt
    11401 aggttgtgcc atcttccaga ataagtgaga cgctcatggt gtcactgttg gtcgccagtg
    11461 actgtttacg cagacgtaat aaatcgacac tggagcgagt gaggtcgaca tacattgtat
    11521 ccagaccacg aatagtcgtt aacgctgtat cttgcgacgc ggtcaccagt gcgccagggg
    11581 ttaccgacga aatgccaatg cgccctgaaa tcggtgcggt taccgtggtc cagtcaagat
    11641 taatgcgcgc agtttccagt gcggcttttt tcgccgctac actggcttta tcttgtgcac
    11701 aggtagactg agcatcatca gcatcctgtt gtgaaacacc gttctctttc accagtcggg
    11761 cataacgctg cgctttctgg caatcggctt ttaccagtgc ctgtgcttgt tgtaatgctg
    11821 cccgagcttc attccatgca gcctgataac tggccgcatc aatctggtag agcggctgtc
    11881 cagccttgac cagatcacct tccttaaata agcgtttctg gataattccc ccaacctgcg
    11941 gacgtacttc ggcactgagc gcagcactgg tgcgtccggt taattcactg actaccgaga
    12001 ccggttgaga tttgactgta acaacgccga cttctgtttc tgacggggat gaattgttac
    12061 tttgcgtatt atcacaacca gataagagca gcattgccac tacagatgtt gctatatact
    12121 tcattatttt ctccagggtg agaaataccc tcctcattaa aattaatgag gatatgtcag
    12181 ggatattcag ttataacgaa cccgcatcgc gaaatgaatt tcgcaatgaa ataaggggga
    12241 ttaatatccc ccgctattgg ttaattctgt tcttatttat ttatcaacat actaacaata
    12301 tcctgatata tttcctgcag atgttgtgga tcgctttttt gcggtgttaa acgacgataa
    12361 accgtacctt ccatcatggt agcaaacact tcaacgcagc aacgaatgtg ctcctcgcta
    12421 agatggggat gcattctttt catatgtgca caaccgttag cgaacattct ggcgtcagct
    12481 tcttcaagca ttgccataac ccgcgggttg cgtgtggctt ctgcagccac ttccagcatc
    12541 agcgcgtcgt cgtcctcatt taaggattta cgcaaggcaa ggacttccgg gaagtgatcg
    12601 gtacgcgcgt caatgtccat ttgggcaata cgaaagtcga tgatacggcg aaccatctct
    12661 tcaataatgg cgtctttgtt ggcgaagtaa cgatatatct gacccacgct aagctgagct
    12721 tcgctggcaa tttgcgacat actggcggca tgaaacccgc agcgacgaaa acaccgacgg
    12781 gcggcgttaa tgatttcatc ctgacgctgt ttgtggcgta tttccagctt gctggtcatt
    12841 tctgtctcct ggcctatcac ttggtgagaa tgatcgttct catttttgct gattatacgt
    12901 ttctccaaag taatggaaag tatttttatc ttattcatgg tgcatattct gataatgaag
    12961 agattgtgga gattctgtga agatagttga caatattaag ttggggtcaa ttcatcagaa
    13021 aggctgctac ttgttgtcgc cagttctgtt ttcatcatca aacttatcgg agctaatacg
    13081 aatagtttca tagtcagatt ctataagata tttccccaac ctaccccttg caggcgtcaa
    13141 gagtagataa cgcaaaatgc tctgtaccgc agcttctctc cggtacagaa agcgcaacta
    13201 tagccaccca cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa
    13261 acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg
    13321 gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct
    13381 acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg
    13441 acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc
    13501 aatgcgatgt tgccgaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt
    13561 ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg
    13621 gtgactatgt taacgccgtt actcgtgaag gcttcaaaat tgcccacgac atcagctcct
    13681 acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc
    13741 tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg
    13801 caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg
    13861 tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag
    13921 acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta
    13981 ttgaagatgt gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg
    14041 gtgaagtggt ccacgttgac ggcggtttca gcattgctgc aatgaacgaa ctcgaactga
    14101 aataatcgtt ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc
    14161 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    14221 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    14281 ctggtatcat gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga
    14341 agccgcaaag gtcagcgatc cctttctact cgacgtgatc cttgaaaaag aaacgctggc
    14401 ccccttcctt tcatggctgg accctgcgcg tgttcttgca gtggagttgt tccctgacca
    14461 gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggccctgac
    14521 ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc
    14581 gctccccggg cctgattcca ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
    14641 tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa
    14701 tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg gttttattgg
    14761 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt
    14821 acccgggctg gagatacaaa actacgccga cgcgtcactg tttgctgatg aatcattacg
    14881 ctggcgaagc agctatgagg tggaaaaact acctgaagaa cacacaaaaa gttcaactgc
    14941 agaactttgc caactggccg aaatactctc tctccaccct atttcacttt cccttctcga
    15001 aaggttgtta aacagcacac gggttaatac acagccggat aatcagcttg cggcgttgtt
    15061 atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt catcaaatga
    15121 atataaaaaa atcagccccc ttctgcgcaa gggcgaaaat gaaattaacc aatgggctga
    15181 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
    15241 tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat
    15301 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    15361 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    15421 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    15481 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggtt
    15541 ttggcttcct ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa
    15601 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    15661 cagagccaga agaactggtt gaaccgtttc tgactcgttt cgtgggtaaa gttcagggca
    15721 aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc
    15781 gcgcagcccg tggtctgaac cacgagttta aagaaggcga ctgggcagtt gccgaaatgc
    15841 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    15901 ttggtgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag
    15961 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt aaagatctga
    16021 ccgcgctgga ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt
    16081 tcgctaaggc gttgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg
    16141 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    16201 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    16261 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca
    16321 ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    16381 atgaccaggt ttctgactgg ctggagaata ccggtgactg gcagcctgaa agtgaagcaa
    16441 ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata
    16501 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    16561 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    16621 tgattgccgc taacatttgt gcagctcgcg tactgcgcga taagctcggt tttggtatct
    16681 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
    16741 cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc
    16801 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    16861 catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    16921 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    16981 cggttatcaa aggcgaaact gcgacgcgtc cacaggatga aatcactgtc caaatggccg
    17041 agcgtcgccg tctcaatcgg atggcagaac gtgatgttgg tgactggtta tacgcacgct
    17101 tcctgaaaga caaagccggg accgacaccc gtttcgcagc ggaaattgtc gatatcagcc
    17161 gtggcggcat gcgtgttcgt ttggttgata acggcgctat cgcctttatt cctgcacctt
    17221 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcacc gtacaaatta
    17281 aaggtgaaac ggtttataaa gtaactgacg ttattgacgt caccattgcc gaagtccgca
    17341 tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct
    17401 tatgaatggg ccgtttattt ccccgctcta ccttcatcat atcccggcag ttttttaact
    17461 gactttcgtt tgaaaactgg cgtttttcca aaactagatt acaaataaat atataaaaaa
    17521 aacaatgtgt ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg
    17581 cggactccgc tgttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt
    17641 tgtacaactt tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgttt
    17701 tgcgcttttc taccaccgaa accacagaac ctgaacgtac gttgcagtta tctgccgaac
    17761 aggctgctcg catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg
    17821 tcgatgagag cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgggaatggg
    17881 ctggcaacgc gtctgcatgg catgacacac cagcagttgc tcgtgattta tcacacggac
    17941 tttcctttgc tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc
    18001 gggggaatat ccaacgcttc aatcggttat gtgaagatta cacagggttg aacgaacacg
    18061 acgtcattgg ccaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca
    18121 ggcgcaataa ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac
    18181 caacacgtaa aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg
    18241 gcaaaaacga gattttttta atctgttccg gcaccgacat taccgaagag cgccgcgctc
    18301 aggaacgact gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg
    18361 caatgcagga gttaatcgat cacgctatta atcaggcaga taacaataaa gttggggtcg
    18421 tgtatcttga tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttatttggtg
    18481 accagttatt acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggtgt
    18541 tggcgcgtcc aggtggcgat gagtttctgg tactggcatc caacacctca caaagcgcgc
    18601 tggaagcaat ggcatcacga attttgaccc gcttacggct cccctttcgc attggtttaa
    18661 ttgaagttta taccagctgt tcagtaggta ttgcactctc tcccgaacat ggttcggaca
    18721 gcgcggctat tattcgtcac gccgacacag caatgtacac agcaaaggaa ggcggacgag
    18781 gacaattttg cgtttttacc ccagaaatga atcaacgggt atttgaatat ctctggctgg
    18841 ataccaactt gcgtaaagca ctggaaaacg atcagttggt tattcactat caacccaaaa
    18901 tcacctggcg tggcgaagtg cgcagtctgg aagcactagt acgttggcag tcacctgaac
    18961 gtgggttgat tccaccgttg gacttcattt cctacgcaga agagtcaggg ctaattgtgc
    19021 ctttaggccg ttgggtgatt ctcgatgtcg tacgccaggt ggcgaagtgg cgggataaag
    19081 gcattaacct gcgagtggcg gtaaacattt ctgcacgtca gctcgccgat caaaccattt
    19141 tcaccgccct gaaacaggtt ctccaggaac tcaattttga atactattga tgttgaattg
    19201 acagagagtt gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc
    19261 caactaggtg cgcaagtgca tctggatgat tttggtaccg cctactcttc actttcgcaa
    19321 ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac
    19381 aaacaacctg tctcgcagtc actggttcgg gcaatcgtcg ctgtggccca ggcattgaat
    19441 cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaat
    19501 gggatcaatg agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa
    19561 cgctggtata aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta
    19621 tattccgtca ttatatatcg tattcccgga tgataattag ccgttgctgt taaacacttc
    19681 tcttttagcg gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg
    19741 atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt
    19801 gtggcagacg attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg
    19861 cgccaggttg ctgaatctac gccagacatc ccgaaggcac cgtgggagat ttaacaatcg
    19921 tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt
    19981 ataacgttat aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca
    20041 ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta
    20101 ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct
    20161 ttgcagtttc gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga
    20221 agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg
    20281 aaaatggcag cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca
    20341 ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtta
    20401 ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggcccg ttgacacgcc
    20461 agtgcatcca acaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg
    20521 aaactggatt caccggtcgc gatatgatgc gtaccgatgt ggtgaatgcc gtgctggaaa
    20581 aagagtgcga agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact
    20641 ccatcggtcc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg
    20701 atctgatgca tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc
    20761 ccttctctac ggatgatttg cagtttgaca aatcatccgc tctaagatga ttcctggttg
    20821 ataattaaga ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat
    20881 ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcgg atttcacgga
    20941 ataatttcac caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc
    21001 ataaattcag gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg
    21061 gcaattaccc tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac
    21121 accgcaatcg gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg
    21181 ttgggtacat taggtggcgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa
    21241 tcgtaaataa atgtcgatta tcgactcgta attataatac gcttgattct gttcagta
//
LOCUS       DBFIFS010000057        21126 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.057, whole genome shotgun sequence.
ACCESSION   DBFIFS010000057 DBFIFS010000000
VERSION     DBFIFS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.057"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            133..273
                     /locus_tag="N7A07_001995"
                     /pseudo
     CDS             133..273
                     /locus_tag="N7A07_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(298..612)
                     /gene="fliE"
                     /locus_tag="N7A07_001996"
     CDS             complement(298..612)
                     /gene="fliE"
                     /locus_tag="N7A07_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU2476663.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            827..2485
                     /gene="fliF"
                     /locus_tag="N7A07_001997"
     CDS             827..2485
                     /gene="fliF"
                     /locus_tag="N7A07_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU2476664.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2478..3473
                     /gene="fliG"
                     /locus_tag="N7A07_001998"
     CDS             2478..3473
                     /gene="fliG"
                     /locus_tag="N7A07_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU2476665.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3466..4152
                     /gene="fliH"
                     /locus_tag="N7A07_001999"
     CDS             3466..4152
                     /gene="fliH"
                     /locus_tag="N7A07_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU2476666.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4214..5587
                     /gene="fliI"
                     /locus_tag="N7A07_002000"
     CDS             4214..5587
                     /gene="fliI"
                     /locus_tag="N7A07_002000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU2476667.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5606..6049
                     /gene="fliJ"
                     /locus_tag="N7A07_002001"
     CDS             5606..6049
                     /gene="fliJ"
                     /locus_tag="N7A07_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU2476668.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6046..7173
                     /gene="fliK"
                     /locus_tag="N7A07_002002"
     CDS             6046..7173
                     /gene="fliK"
                     /locus_tag="N7A07_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU2476669.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7278..7742
                     /gene="fliL"
                     /locus_tag="N7A07_002003"
     CDS             7278..7742
                     /gene="fliL"
                     /locus_tag="N7A07_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU2476670.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7747..8751
                     /gene="fliM"
                     /locus_tag="N7A07_002004"
     CDS             7747..8751
                     /gene="fliM"
                     /locus_tag="N7A07_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU2476671.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8748..9161
                     /gene="fliN"
                     /locus_tag="N7A07_002005"
     CDS             8748..9161
                     /gene="fliN"
                     /locus_tag="N7A07_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU2476672.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9164..9529
                     /gene="fliO"
                     /locus_tag="N7A07_002006"
     CDS             9164..9529
                     /gene="fliO"
                     /locus_tag="N7A07_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU2476673.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9529..10266
                     /gene="fliP"
                     /locus_tag="N7A07_002007"
     CDS             9529..10266
                     /gene="fliP"
                     /locus_tag="N7A07_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU2476674.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10276..10545
                     /gene="fliQ"
                     /locus_tag="N7A07_002008"
     CDS             10276..10545
                     /gene="fliQ"
                     /locus_tag="N7A07_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU2476675.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10553..11338
                     /gene="fliR"
                     /locus_tag="N7A07_002009"
     CDS             10553..11338
                     /gene="fliR"
                     /locus_tag="N7A07_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU2476676.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11628..12251
                     /gene="rcsA"
                     /locus_tag="N7A07_002010"
     CDS             11628..12251
                     /gene="rcsA"
                     /locus_tag="N7A07_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU2476677.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12295..12483)
                     /gene="dsrB"
                     /locus_tag="N7A07_002011"
     CDS             complement(12295..12483)
                     /gene="dsrB"
                     /locus_tag="N7A07_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU2476678.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12646..12873
                     /gene="yodD"
                     /locus_tag="N7A07_002012"
     CDS             12646..12873
                     /gene="yodD"
                     /locus_tag="N7A07_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU2476679.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13171..13986
                     /gene="yedP"
                     /locus_tag="N7A07_002013"
     CDS             13171..13986
                     /gene="yedP"
                     /locus_tag="N7A07_002013"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU2476680.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13983..15677)
                     /gene="dgcQ"
                     /locus_tag="N7A07_002014"
     CDS             complement(13983..15677)
                     /gene="dgcQ"
                     /locus_tag="N7A07_002014"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU2476681.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15848..16030)
                     /gene="yodC"
                     /locus_tag="N7A07_002015"
     CDS             complement(15848..16030)
                     /gene="yodC"
                     /locus_tag="N7A07_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU2476682.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16109..17026)
                     /gene="yedI"
                     /locus_tag="N7A07_002016"
     CDS             complement(16109..17026)
                     /gene="yedI"
                     /locus_tag="N7A07_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU2476683.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            17199..18119
                     /gene="yedA"
                     /locus_tag="N7A07_002017"
     CDS             17199..18119
                     /gene="yedA"
                     /locus_tag="N7A07_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU2476684.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18108..18578)
                     /gene="vsr"
                     /locus_tag="N7A07_002018"
     CDS             complement(18108..18578)
                     /gene="vsr"
                     /locus_tag="N7A07_002018"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU2476685.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18559..19977)
                     /gene="dcm"
                     /locus_tag="N7A07_002019"
     CDS             complement(18559..19977)
                     /gene="dcm"
                     /locus_tag="N7A07_002019"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU2476686.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20044..20739)
                     /gene="yedJ"
                     /locus_tag="N7A07_002020"
     CDS             complement(20044..20739)
                     /gene="yedJ"
                     /locus_tag="N7A07_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU2476687.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20779..21059)
                     /gene="drpB"
                     /locus_tag="N7A07_002021"
                     /pseudo
     CDS             complement(20779..21059)
                     /gene="drpB"
                     /locus_tag="N7A07_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5090 a         5618 c         5456 g         4962 t
ORIGIN      
        1 ataaaattac cactatcaaa aatcgctcat atccatagcc cggaacatga ataaaaacat
       61 caacaaagat aaaattatcc agaggttact gttgcgtctg tggtcgtaac aacatccgac
      121 gtggtgatgt gtcaaatgag acactctgat gttcaggggg tttatcgtgt atacggatcg
      181 tggatggcag aaaactttca ggatcaggta tccatttcaa accagataat gagtaagttt
      241 gccccatcca tgccccacgc ggtgagatcg gatgtcatta acaacaggtt acacaaccta
      301 tacctgcatg ctcattactt cctgatatgc cgccaccagc ttattacgca cctgaatccc
      361 catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta acgccacgcc
      421 gggttcaccg agagtgaatt tttctgcctg cgtgcgggcg gctgtttgtg tatcgcttat
      481 gcgatcgagc gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgattc
      541 ctgcgcacgc gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg
      601 tatcgctgac attttcatct cctggataat ttctggtagc aaagcctacc agtaagtcaa
      661 taagacaaag gcgctaaata gcaacaaaaa aacgggttta ttggcggata gaaaaaaacg
      721 aaagcacaaa taatgggagc gtcaattttt cgagtttgct gacccgggag tgagtcttgt
      781 tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc
      841 agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt
      901 gattgttgcc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc
      961 ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag
     1021 ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaagt
     1081 tccggcagat aaagttcacg agctgcgcct gcgcctggcg caacaaggtt tgcccaaagg
     1141 cggcgcggtc ggtttcgaac tactcgatca ggaaaagttt ggtatcagcc agttcagcga
     1201 acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg
     1261 cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga
     1321 acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga
     1381 agggcaaatt agcgccattg tgcatctggt ttccagcgcc gttgctggtc tgccgccggg
     1441 aaacgtcacg ctggtggatc agggcggaca tctgttaacc cagtccaata ccagctggcg
     1501 cgatcttaat gacgctcaat tgaaatatgc cagcgatgtc gaaggccgta ttcagcggcg
     1561 tattgaagca atcctgtcgc ctattgttgg taacggtaat attcacgccc aggtcacggc
     1621 gcaactggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta atggtgatga
     1681 atctcaggcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta
     1741 tccgggcggc gtaccggggg cgttgtcgaa tcaaccggct cctgcgaata acgcgccaat
     1801 cagcacgcct ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag
     1861 tgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcactattcg
     1921 tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta
     1981 caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga aacaaattga
     2041 agatctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt
     2101 taactcgccg ttcaatagta gtgacgaaag cggcggagaa ctgccgttct ggcaacagca
     2161 agcgtttatc gatcagttac tggctgccgg tcgctggttg ctggtactgc tggtggcgtg
     2221 gctgctgtgg cgtaaagcgg tacgcccgca gctaacacgt cgcgctgagg cgatgaaagc
     2281 tgtacagcaa caggcgcagg cccgcgagga agtggaagat gcggtggaag tccgcctgag
     2341 caaagacgaa caactccaac aacggcgcgc taaccaacgt ctgggggcag aagtcatgag
     2401 ccagcgtatc cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca
     2461 gtggataaat aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga
     2521 tgaccattgg cgaagaccgg gcggcagaag tgttcaagca cctctcccag cgcgaggtgc
     2581 aaaccctgag cgctgcaatg gcgaacgtca cgcagatctc caacaagcag ctaaccgatg
     2641 tgctggcgga gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg
     2701 attatctgcg ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg
     2761 aagatattct cgaaactcgc gataccgcca gcggtattga aacgctcaac tttatggagc
     2821 cgcagagcgc cgccgatctg attcgtgatg agcatccgca aattatcgcc accattctgg
     2881 tgcatctgaa gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc
     2941 acgacgtgat gttgcgtatc gccacctttg gcggcgtgca gccagccgcg ctggcggaac
     3001 tgaccgaagt actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg
     3061 gcgtgagaac ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa gaagccgtta
     3121 ttaccgccgt gcgtgaattc gacggcgagc tggcgcagaa aatcatcgac gagatgttcc
     3181 tgttcgaaaa tctggtggat gtcgacgatc gcagcattca gcgtctgttg caggaagtgg
     3241 attccgaatc gctgttgatc gcgctgaaag gggccgagca gccactgcgc gagaaattcc
     3301 tgcgcaatat gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc
     3361 cggtgcgtct gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc
     3421 ttgccgaaac tggcgagatg gtaattggca gcggcgagga tacctatgtc tgataatctg
     3481 ccctggaaaa cctggacgcc ggacgatctc gcgccaccac aggcagagtt tgtgcccatg
     3541 gtcgagtcgg aagaaaccat cattgaagag gctgaaccca gccttgagca gcaactggcg
     3601 caactgcaaa tgcaggccca tgagcaaggt tatcaggcgg gtattgccga aggtcgccag
     3661 caaggtcata agcagggcta tcaggaagga ctggcccagg ggctggagca aggtctggca
     3721 gaggcgaagt ctcaacaagc gccaattcat gcccggatgc agcaactggt cagcgaattt
     3781 caaactaccc ttgatgcact tgatagtgtg atagcgtcgc gcctgatgca gatggcgctg
     3841 gaggcggcac gtcaggtcat cggtcagacg ccaacggtgg ataactcggc actgatcaaa
     3901 cagatccaac agttgttgca gcaagaaccg ttattcagcg gtaaaccaca gctgcgcgtg
     3961 cacccggatg atctgcaacg tgtggatgat atgctcggcg ctaccttaag tttgcatggc
     4021 tggcgcttgc ggggcgatcc caccctccat cctggcggct gtaaagtctc cgccgatgaa
     4081 ggcgatctcg acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca
     4141 ggagtggtgt aatgaccacg cgcctgactc gctggcaaga actctgccgt ctggcagcac
     4201 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     4261 agccaaaatg gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg
     4321 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     4381 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     4441 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     4501 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     4561 attaggtcgc gttctggacg gcagcggtaa accgctcgat ggcctgccct cccccgatac
     4621 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     4681 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     4741 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     4801 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     4861 aaaagatttt attgagaata tcctcggtgc cgaagggcgt gcacgatcag tggtgattgc
     4921 cgctccggcg gatgtgtctc cgctcctgcg aatgcaaggt gccgcctatg ccacgcgcat
     4981 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     5041 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     5101 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     5161 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     5221 ggaccccatt gcagactccg cgcgggcgat cctcgacggt cacattgtgc tgtctcgccg
     5281 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     5341 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     5401 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     5461 gatgctcgac aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat
     5521 ttttgaacgc gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     5581 gtcataaccc aggagataac ggcagatggc agaacatggc gcgctggcga ccctgaaaga
     5641 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     5701 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     5761 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     5821 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     5881 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     5941 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     6001 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     6061 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     6121 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac taccgacaaa
     6181 gcggcccccc agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctgatc
     6241 agcgatattg tttccgacgc gcaacaagct aatttactga tccctgtgga tgaaacaccg
     6301 cctgtcatca acgacgaaca atccacatca acaccgttaa ccaccgctca gacgatggcg
     6361 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     6421 gacgtcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     6481 aaagtgactg atgtgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     6541 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac accagctcag
     6601 ccattaacac cgcaggtagc agaagcccag agtaaagcgg aagtcatcag cacgccttcg
     6661 ccggtgactg ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc
     6721 gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     6781 cagcatattt cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcacccg
     6841 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     6901 atggtttcat cgcatcagca tgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     6961 acgcagctgg ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     7021 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     7081 cctctggcgg gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     7141 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     7201 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     7261 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     7321 ggatcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     7381 ggcattcgca tcaggttgct gctgacgaca aagcgcagca acgcgtcgtg ccctccccgg
     7441 tcttctatgc gctggatacc tttacggtca atctgggcga tgcggatcgc gtgctttata
     7501 tcggcataac cctgcgcctg aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     7561 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     7621 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacactttcc accccgcttg
     7681 ttgccgggca accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat
     7741 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     7801 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     7861 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     7921 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     7981 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     8041 ccgaccaacc tgaaccttat ccatctaaaa ccgctgcgcg gcactgggct ggtggtgttc
     8101 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg
     8161 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgttg
     8221 aaactggcgc ttgaaggcta tagcgacgca tggaaggcga ttaatccgct ggaagttgag
     8281 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     8341 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgc
     8401 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     8461 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     8521 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     8581 aaacccggtg acgtcctgcc gatagaaaaa cccgaccgca tcatcgccca tgttgacggc
     8641 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     8701 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
     8761 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
     8821 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
     8881 gtgatgtcag cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga
     8941 ccgtcgagct gggccgcaca cgaatgacca tcaaagagct gttgcgtctg acgcaagggt
     9001 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
     9061 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
     9121 tcattactcc gtctgagcga atgcgccgcc tgagccgtta gtgatgaata accacgctac
     9181 tgtgcaatct tccgcgccgg tttctgctgc gccactgctg caggtgagcg gcgcactcat
     9241 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
     9301 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
     9361 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
     9421 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
     9481 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagcggg agatcctgat gcgtcgttta
     9541 ttgtctgtcg cacctgtcct tctctggctg attacgcccc tcgccttcgc gcaactgccg
     9601 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
     9661 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
     9721 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacacc ctccgcgcca
     9781 cctaaccagg tattgctggg gctggcactg tttttgacct tttttattat gtcaccggtg
     9841 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
     9901 gaggcgctgg aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag
     9961 gcagatttag ggttgtttgc cagactggcg aataccggcc cgttgcaggg acctgaagcc
    10021 gtgccgatgc gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag
    10081 ataggcttca cgattttcat cccttttttg attatcgacc tggtgatagc cagcgtgttg
    10141 atggcattgg ggatgatgat ggttcccccc gccaccattg ctctgccctt taaactgatg
    10201 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    10261 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    10321 gtcgcgctgg cactggctgc cccgctattg ttggtagcgt tggtcacggg ccttatcatc
    10381 agtattttgc aggccgccac gcagattaac gaaatgacgc tgtcgtttat tccgaaaatc
    10441 atcgccgtat ttatcgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    10501 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtagccgta ctatgttgca
    10561 ggtgacaagc gaacaatggc tatcctggtt aagcctgtac ttctggccgt tactgcgcgt
    10621 gctggcgctg atctccaccg cgccgattct gagcgaacgc agcgtaccga aacgggtaaa
    10681 actgggtctg gcaatgatga tcacgttcgc cattgcccca tcattacctg ccaacgatgt
    10741 tcctgttttt tcgttctttg ctctgtggct ggccgtgcag cagatcctga tcggcattgc
    10801 gcttggtttt accatgcaat ttgcctttgc cgctgtgcga accgctggcg aaattatcgg
    10861 tctgcaaatg gggctgtcat ttgcgacgtt tgtcgatccg gccagccatc ttaatatgcc
    10921 cgttttagcg cgtatcatgg atatgctggc gttactgctg ttcctgacat ttaacggtca
    10981 tttatggttg atttcactgc tggtcgatac ctttcacacc ctgccgattg gtggcgaacc
    11041 gttgaacagc aatgcgtttc tggcactcac caaagcaggg agtttgattt tccttaacgg
    11101 gctgatgctg gcgttaccgc tcattactct gctgctgaca ctgaatctgg cattaggttt
    11161 acttaatcgt atggccccgc aattatccat ttttgttatt ggatttccat taactctgac
    11221 tgtcggcatc tctttaatgg cggcattaat gccgttaatt gcgccttttt gcgaacattt
    11281 attcagtgaa atttttaatt tgctggctga tattattagt gaattgccat taatataatt
    11341 ccgtaacgtt tatcatgtta tcctaaggat tatccgaaaa ataataccta cgaacatctt
    11401 ccaggatact cctgcagcga aatatttgtt ttaagctcac tcacatatcg caacatttac
    11461 tttactttaa gacaattcca ggcaaattat acaacacttt acgggatagt aagtccgcct
    11521 gaaaaatcgc gagagtggcg cattaggtga cccatgttgt tccgtttagt catgatgaaa
    11581 tattcaggta aggggaatta tcgttacgca ttgagtgagg gtatgccatg tcaacgatta
    11641 ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg agtagagggg
    11701 ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata gcttgtgatt
    11761 cacagcgccc ttcagtggtg tttattaatg aggactgttt catccacgat gcttctaaca
    11821 gtcagcgtat caagctcatc attaatcaac atcccaatac gttatttatc gtttttatgg
    11881 caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta ttgatcagtt
    11941 ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg aaaaaagaga
    12001 caacgataac ctcgttttta aatatgccga cgttatcatt gagccgaacc gaatcgagta
    12061 tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctccgaccaa atgaatatca
    12121 aagccaagac cgtttcatcg cataaaggta atattaaacg taagatcaaa acgcataata
    12181 aacaggttat ctaccatgtc gtccgactga cggataatgt gactaatggt atttttgtca
    12241 acatgcgcta acacattctg actggtggtt tcccaccagt caggctgaat tagattactc
    12301 tgctttctcc acaaagatac cgtcctgatg ccctgcttca ttaaagaacc agatgccgag
    12361 cgggtagtct tccagcgaaa ccaggtacat tgtgccttca ctaaactcct caactgccag
    12421 taccacgcca ggacgacgcg gaccgccatc cgttttgact gttacccgat cattcacctt
    12481 catcgttttc ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc
    12541 caggcgcgcg cgagtgctga ttttctcgac ggtctatact taagagatgc caggcggact
    12601 taacgactgg cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa
    12661 gagtacagcg ataccgcaaa acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg
    12721 atcaatgaaa ttagcgaaag cgaagttcat cgcagccaga acgattctga acacgttagc
    12781 gtcgatggac gtgaatatca tacatggcgt gaattggcgg atgccttcga actggatatt
    12841 catgacttca gcgtctctga agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg
    12901 agggggtcgg gatgaaactt gcttaagcaa gaagcactta aaaaattcgt tacaccagga
    12961 aatctgatgt gttcatcacc ttatccgcaa tttttttcgc tgacaagaaa atattcgcta
    13021 tatgaatgat taacgctact tatgaataga aatgtgacgc acgtcacccc ttggtggctc
    13081 tggcgtcagc gggatcgctc ccgaaaaaat catctttgat aatttgtgct gttttgagaa
    13141 tcatcctggt aaggggagta ctgcgcgact atgttttcaa ttcaacaacc actactggtt
    13201 tttagcgatc ttgatggcac cctgctggac agtcatagtt atgactggca accggcagcc
    13261 ccctggctca gccgtttacg cgaagcaaat gttcccgtca ttctctgtag cagtaaaaca
    13321 tcagcggaaa tgctgtactt gcaaaaaatg ttggggctac aaggtttacc gctgattgca
    13381 gagaatggcg cagtgatcca gcttgctgaa caatggcagg agatagacgg ttttccacgc
    13441 atcatctcag gtattagcca tggcgaaatc agccaggttt taaatacgct acgcgagaaa
    13501 gaacatttta aattcacgac ttttgatgat gtcgacgatg caaccatcgc cgaatggacg
    13561 ggattaagcc gtagccaggc ggcgctgacg cagcttcatg aggcgtcggt aacgctaatc
    13621 tggcgcgaca gtgacgagcg tatggcacaa tttaccgctc gtctgaacga actgggctta
    13681 cagtttatgc aaggtgcgcg cttctggcac gtcctggatg cctctgccgg aaaagatcag
    13741 gctgccaact ggattatcgc gacctatcaa caattgtcag gcaaacgccc aaccacactt
    13801 ggcctgggcg atgggccaaa cgatgcgccc ttactggagg taatggatta cgcggtgatt
    13861 gtgaaagggc taaaccgtga aggggtgcat ctgcatgatg aggatccggc ccgcgtctgg
    13921 cgaacgcagc gtgaaggacc ggaaggatgg cgtgaagggc tggaccattt tttctccgcc
    13981 cgttaagcgt tatcgctcgc gaatacccga ttgcgcccag cctgtttagc gagataaagc
    14041 cgacggtcgg ccagtgactg gagttgttca aaatcataat caccggtttc ctcgctgcta
    14101 cttaccccca acgaggcact gatgcgtatc gtcgtactct tagcaatcaa catctctttt
    14161 tcatttaact tcaggcgaat acgttctgcg acttccgcag cctccgtcag actcgcgcct
    14221 ggcagaatca cacaaaactc ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag
    14281 gaactgctaa ttaatccggc agcatgagaa agaacacggt cgcccgcctg atgaccaaag
    14341 cggtcattaa tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg
    14401 tgcgtctgac acaatttcgc gagcggacgg gctttttcga acagtgcgcc acggttatat
    14461 aaacgcgtta aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag aacatacatg
    14521 ttgctgacca tccggcgaat cacataccag gagatgagta acatggtggt aaagagcgcc
    14581 cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttaac
    14641 gtatggacgc gcaccagcac accgtcgaaa tggtccagac gttcccagct aacatagcga
    14701 ctgtccatac gaatgccgcc ccgcgtgtca tgttccattg cctgcgccag caaggccagt
    14761 tcacgaggat caaaaatatt ccctgttgga tggtcaggat tggaagaggt caaaaatctc
    14821 agcttactgt catagagctg atactcacca tcgaggtttt tatcgatggc gtttcttaaa
    14881 aattgctgca tagtacgcac gggaatactc atccccagca cgccatacca gtagttatta
    14941 ctgtctaccg gaacactgac ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc
    15001 gaagtgaacc agcgtaccgc gcggtgacga ttttctcgtt gtgaatggcc gataaaccag
    15061 ggttgggtga catagccgta ataacgcgtc gggacattgc gcgtaaacaa ggtcggctgc
    15121 gtcgaaacgt aaaatccggc acgcgagaca tacatcgcct gttctaccat cgacgaggag
    15181 ttgtgtgcca gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc attgtcgagg
    15241 ctttcatttt cgcgagacag gagattcccc tcgctgacta atgcatccga tacgccattg
    15301 actgacaggg tgcgtcgtcg gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt
    15361 tcgaactcgg ttacagcatc acgcagagag gtgaaatcca gtggcgctac gagagcttcg
    15421 cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg cttatcgagc
    15481 gcgttggcta cgttttccag atgattacgc tggctggaga tataggcatc ttccagcacc
    15541 actacttcac gccaggtcag caaggtggaa aaaagcaata ccacgataaa gcagagatta
    15601 acgatatgac caggccctag gcggcgtgcg agttttttca accagctctg gttttccatt
    15661 tttgtctcgt gctgcaccct gacgactccc cacctgcttt tttatgattc tggcataacc
    15721 tggcgatagc gtaccgcaaa acagcgataa ttttataatg gtggcatgat aagacgcgac
    15781 tatcattcat tgcaggcatt ggcacaccag gccggatgtg atccggcctg gtcttaatga
    15841 taccctttca aacttcttcc gcagaacgac tcccctcccc tggaacaagc tcggtttcat
    15901 gaaaagcttc ccgcttgacc ccgtaaccgt cataccaacg acactcaacc ataccgctgg
    15961 agtatccagt gacaatcatc cgcgggccgc cctctttaac cgtaacttcc tcactaacca
    16021 taaagctcat actcgcctcc ttttttcgag tgaaacttgt tcaccttagt tgaagatggc
    16081 gaattttgca taatagcctg tgcgagtatt aatgtacctg accgcgcatt ttcgctacgg
    16141 ctttcactcc cagcaccacg atgccgccga tgataaatcc aagaatcaga tttaaaacag
    16201 tcggtaatat catcgccacc actgcacttt gctgcccggc gaaatgttca atggcgtgat
    16261 gcagcggcgc aataccatgt accacaatcc cgccgccgac gaggaacatc gccagcgtgc
    16321 cgacaatcga taacgctttc atcagccagg gcgcgataat caataatcct ttacctaatg
    16381 cctgcatcag cgcgctggat ttttccgcca gccaataccc caggtcatca atcttaacga
    16441 taacccctac cagaccgtag acgcccacgg tcactaccag cgcgatgcct gaaagcacca
    16501 gcacctgatt aagcaacggc gcttcggcca caatccccag cgtgatggcg acgatttccg
    16561 cagacaatat aaaatcggta cgaatcgccc cttttatttt gtccttttca aacttcagcg
    16621 gatcctgcgc cgccagcttc tccagacgct gctggctctg cgccggatct tctttatgtt
    16681 tacgcgcctc cagcatatgc agcactttct ctaccccttc aaagcagaga aacgcgccac
    16741 cgatcatcaa caatggcgta atcgcccacg ggataaacgc actgatgatc agcgccagtg
    16801 gcaccagaat cactttatta atcagcgatc ctttcgccac gccccagacc acgggaagtt
    16861 cccggttggc ccgcacgcct gaaacttgtt gcgcattgag cgataagtca tcccctaata
    16921 caccggcggt tttcttcgcc gccagtttgc ccatcacgga gatatcgtcc agcagtgtgg
    16981 cgatatcatc gagcaacgtc agtaaactac tcccggccaa aagcattccc tctcattttt
    17041 attgttgaag ggtgtaagta tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc
    17101 aacatttttt taacaatcaa gtgcaattat atctctcgcc agcagaatga ttttcacgtt
    17161 tactatgaac caccttttta tttccaccgt gagggattat gcgtttccgc cagttgttac
    17221 cgctttttgg cgcgctgttt gcgttgtata tcatttgggg ctcaacctat tttgtcattc
    17281 ggattggcgt ggaaagctgg cctccgttaa tgatggcggg tgttcgattc ctggcagccg
    17341 gtattttatt gctggcattt ttactactgc gcggacacaa actccccccg ctacgtccgc
    17401 tgctcaatgc cgcgctgatt ggcctgttat tgctggctgt cggtaatggc atggtgacgg
    17461 ttgccgaaca tcaaaatgtt ccttccggca tcgccgccgt agtggttgca accgtgcccc
    17521 tctttaccct gtgcttcagc cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg
    17581 gtattgccat tgggcttgcc ggaatcatca tgctcaatag cggtggaaat ttaagcggca
    17641 atccgtgggg cgcgattctg attttaatcg gctcgattag ctgggcgttt ggctcagttt
    17701 atggctcgcg cattacctta cctgtaggga tgatggcggg tgcgattgag atgctggcgg
    17761 caggcgtggt gttaatgatc gcgtcgatga ttgcgggtga aaaactgacg gcgctccctt
    17821 ccctttcagg cttccttgcg gtcggctatc tggcgctgtt tggttcgatt atcgccatca
    17881 acgcttatat gtatttaatc cgtaatgtca gtccggctct cgccaccagc tacgcttacg
    17941 ttaacccggt ggtcgcggtc ttgctgggta cgggactggg tggagaaaca ctgtcgaaga
    18001 ttgaatggct ggcgctcggc gtaattgtct tcgcggtggt actggtcacg ttgggaaaat
    18061 atctctttcc ggcaaaaccc gtagttgctc cagttattca ggacgcatca agcgagtaaa
    18121 tgtatcccct gcgtgtcgat ctgcgcgctg gctccttcgc cgcagatcca ctcttccaga
    18181 cgctcggtaa gcgcttcatc cttcagcttc tcgcgcccac gtaacgcgca ctcccagaca
    18241 atcaatacgc gccagccgag ttcctgcaag cgactgatat cgcggcgatc gcgctcaaca
    18301 tttttaccta tcttctccag ccagaactcg gttcgagtcg caggcacttt aaacagatag
    18361 cagtgatgat gatgccagaa gcagccatgg gtaaatatca cgcagcgata ttcatcaacg
    18421 acaaaatccg ggcgtccggg cagactggcg tcctgaacgc gaaacgccag gccctgcccg
    18481 gttaacagac tggcgaggcg cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata
    18541 tttttgctgc gagtggcctt atcgtgaacg tcggccatgt tgtgcctctt gctgacgcaa
    18601 cgccaccgcc tgtttgattt ttggctcaag cagttttgcc accgcggcaa agaccggcac
    18661 gaccaccgag ttaccgaact ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt
    18721 cgcttctccc ggcgcttcaa aacccattaa gcgcgcgcat tcccgaggcg ttaaccgacg
    18781 tggacgatgt tgctgattca gcggatcgtc aaagtctttc tcacccgtgg ccatatccca
    18841 gccgcgatcg attaaaattt ccgcgccatc tttgtaataa cgcgcagaca gcgtacgggt
    18901 gacgctttgc ggattgttcg gataaaccat tccataaccg aagccgtttc cgcgcgcctg
    18961 atgttttttc gcatatcgat agaggtactt ccacagcacc ggcgtcagga tatatttcgc
    19021 ctcgaccatc gggtccaaca gctgcgccag cgtcactcgc tgtgcaggga aacattcgct
    19081 gatatcacgc agagtaaaat cggctttcag attaagatcg cgacgaaaac ccaccagcac
    19141 gatgcgttca cggtgctgcg gcagaaaatg tttgccatcg atgattttcg gatcgtccgg
    19201 cccgttatct tctgcatcag ccacgtcata gcccagttcg tccagcgtct gcatgatgat
    19261 gcggaacgtt ttaccctggt cgtggctttt caggtttttg acgttttcga gcacaaacat
    19321 cgccggacga cgcgcgtcga taatgcgcac cacatcaaag aacagcgtgc cctgagtatc
    19381 gcaggcaaaa ccgtgcgccc gcccgagcga gttctttttc gatacgccag ccagcgaaaa
    19441 tggctgacaa gggaaaccgg ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg
    19501 ttccgccgca gcctcatcac tcacgccttc tttatggctg agggtgatgt cgcggatatc
    19561 ttcattaaaa tgatgcgtcg ccgggtcgca ataatgattg gctttataag tgcgtaccgc
    19621 atgtttgttc cattcgctgg taaacacgca ctgtccgcca atcgattcaa aaccgcgacg
    19681 gatgccgcca attccggcaa atagatcgat aaagcgaaac gcataatgcg gatgatgtgc
    19741 cggtggtttg ggtaacaacg tttgcagatg ggcgaactct ttctcactta aacggtgcca
    19801 tgccgagtca ttcgccagcg cacgttttaa aattgccgcg ctccagtgat tctcacccac
    19861 accattaagc tgagccacca gcgttttaac atcataaatt tgcagcagtt tctccagcat
    19921 tgcctgtgcg gcattcccgg tgctgtatga atcggttact gatatatttt cctgcataga
    19981 tttaaccggc catctgagat ggtcgacagg ttaacataat tacagccatt tagggccacg
    20041 cgctcagccc gcgggtgaaa acgcatctat caccttgtga tcgacacctt cattctcccc
    20101 cgccagttcg gcactgagct tcgccataaa ctcgactaaa aaatgcgcgt tgtgctgcgc
    20161 cagctgcttg cctcttacgg tttgcatgat ttgcggtagc ttcaacaact tagtctggaa
    20221 atgatccagc gcgtagcgtt tatcatcaag cgggcgatgc tgtgcgagcg gatcttcggc
    20281 atcaaacagc gccacgccca atgcccctga aacggcaaac acgcgcgcca ggccaatcgc
    20341 ccccagggct tccagccgat ccgcatcctg gacgatttta gcttccgttg ttaagggggc
    20401 gatttgcgcg ctgaaactgt gagcggcaat ggcatgacaa acggcctcga ttttctccgc
    20461 cggaaattgc acgaactctt cacggagcag gcgacgcgtc tcttctgccg ccaggattga
    20521 agaactctgc cgttgcggat gatttttcgc caggctcaca atatcgtgga aataacaggc
    20581 ggttaaaatc accagcatat cgacgtcatc atctgccgcc agcttctgtg ccgtagccca
    20641 gacgcggcga aaatgacaca cgtcatgggc cgcgtcctgg tgctgatgat ggtttttcaa
    20701 ccagttttcg aactgtgcct gccagtgttg taagtccata cgcgctccgt tgccaaagag
    20761 tcgccacctt agcagttttt attcatagcg tctgctacgt gcgggacgag gtcgtgtgta
    20821 ccaggcaatg ctgcctaaca atgccccgac cagagccata aataaaaatc cgaccagtgc
    20881 cctaaccact ttcccgcggt agagcctaaa tcgctttcaa atcccgacac gggggcatcg
    20941 gggatccggc tcatcaccca gatgtaacgc gccattgccc agacgaaata gccacaccat
    21001 gtataaaaag cccacaaagc cagtttgccg ccagggctgc gagagagtct ctcttccatc
    21061 tttgtcgaac cgtattccat tttgagataa cccggaaaat tcagacttag tactattacg
    21121 tgataa
//
LOCUS       DBFIFS010000058        21012 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.058, whole genome shotgun sequence.
ACCESSION   DBFIFS010000058 DBFIFS010000000
VERSION     DBFIFS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.058"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            66..296
                     /gene="holE"
                     /locus_tag="N7A07_002022"
     CDS             66..296
                     /gene="holE"
                     /locus_tag="N7A07_002022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU2476688.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            398..1054
                     /gene="yobB"
                     /locus_tag="N7A07_002023"
     CDS             398..1054
                     /gene="yobB"
                     /locus_tag="N7A07_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU2476689.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            1078..1740
                     /gene="exoX"
                     /locus_tag="N7A07_002024"
     CDS             1078..1740
                     /gene="exoX"
                     /locus_tag="N7A07_002024"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU2476690.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADMTGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1737..3797)
                     /gene="ptrB"
                     /locus_tag="N7A07_002025"
     CDS             complement(1737..3797)
                     /gene="ptrB"
                     /locus_tag="N7A07_002025"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU2476691.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(4006..4665)
                     /gene="yebE"
                     /locus_tag="N7A07_002026"
     CDS             complement(4006..4665)
                     /gene="yebE"
                     /locus_tag="N7A07_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HGU2476692.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(4992..5348)
                     /gene="yebF"
                     /locus_tag="N7A07_002027"
     CDS             complement(4992..5348)
                     /gene="yebF"
                     /locus_tag="N7A07_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU2476693.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(5415..5705)
                     /gene="yebG"
                     /locus_tag="N7A07_002028"
     CDS             complement(5415..5705)
                     /gene="yebG"
                     /locus_tag="N7A07_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU2476694.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            5839..7017
                     /gene="purT"
                     /locus_tag="N7A07_002029"
     CDS             5839..7017
                     /gene="purT"
                     /locus_tag="N7A07_002029"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU2476695.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(7073..7714)
                     /gene="kdgA"
                     /locus_tag="N7A07_002030"
                     /gene_synonym="eda"
     CDS             complement(7073..7714)
                     /gene="kdgA"
                     /locus_tag="N7A07_002030"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU2476696.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(7751..9562)
                     /gene="edd"
                     /locus_tag="N7A07_002031"
     CDS             complement(7751..9562)
                     /gene="edd"
                     /locus_tag="N7A07_002031"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU2476697.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(9797..11272)
                     /gene="zwf"
                     /locus_tag="N7A07_002032"
     CDS             complement(9797..11272)
                     /gene="zwf"
                     /locus_tag="N7A07_002032"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU2476698.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11610..12479
                     /gene="hexR"
                     /locus_tag="N7A07_002033"
                     /gene_synonym="yebK"
     CDS             11610..12479
                     /gene="hexR"
                     /locus_tag="N7A07_002033"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU2476699.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            12607..14049
                     /gene="pyk"
                     /locus_tag="N7A07_002034"
     CDS             12607..14049
                     /gene="pyk"
                     /locus_tag="N7A07_002034"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU2476700.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(14180..15151)
                     /gene="lpxM"
                     /locus_tag="N7A07_002035"
                     /gene_synonym="msbB"
     CDS             complement(14180..15151)
                     /gene="lpxM"
                     /locus_tag="N7A07_002035"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU2476701.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(15271..16593)
                     /gene="mepM"
                     /locus_tag="N7A07_002036"
     CDS             complement(15271..16593)
                     /gene="mepM"
                     /locus_tag="N7A07_002036"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU2476702.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(16609..17541)
                     /gene="znuA"
                     /locus_tag="N7A07_002037"
     CDS             complement(16609..17541)
                     /gene="znuA"
                     /locus_tag="N7A07_002037"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU2476703.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            17620..18375
                     /gene="znuC"
                     /locus_tag="N7A07_002038"
     CDS             17620..18375
                     /gene="znuC"
                     /locus_tag="N7A07_002038"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU2476704.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            18372..19157
                     /gene="znuB"
                     /locus_tag="N7A07_002039"
     CDS             18372..19157
                     /gene="znuB"
                     /locus_tag="N7A07_002039"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU2476705.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(19403..20413)
                     /gene="ruvB"
                     /locus_tag="N7A07_002040"
     CDS             complement(19403..20413)
                     /gene="ruvB"
                     /locus_tag="N7A07_002040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU2476706.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(20422..>21012)
                     /gene="ruvA"
                     /locus_tag="N7A07_002041"
     CDS             complement(20422..>21012)
                     /gene="ruvA"
                     /locus_tag="N7A07_002041"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU2476707.1"
                     /translation="IIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHF
                     VVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGA
                     LVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAAL
                     VALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         4999 a         5466 c         5239 g         5308 t
ORIGIN      
        1 attccggctt gccatccagg ctcactctta gtaacttttg cccgcgaatg atgaggagat
       61 taagaatgct gaagaatctg gctaaactgg atcaaacaga aatggataaa gtgaatgtcg
      121 atttggcggc ggccggggtg gcatttaaag aacgctacaa tatgccagtg atcgctgaag
      181 cggttgaacg tgaacagcct gaacatttgc gcagctggtt tcgcgagcgg cttattgccc
      241 accgtttggc ttcggtcaat ctgtcacgtt taccttacga gcccaaactt aaataaaact
      301 tatacagagt tacactttct tacataacgc ctgctaaatt atgagtattt tctaaaccgc
      361 acccataatt tgcagtcatt ttgaaaagga agtcattatg tcgttctgga aagttgcagc
      421 agcgcaatat gagcctcgta aaacctcact caccgaacag gtagctcatc atctggagtt
      481 tgtgagggcc gccgccaggc aacaatgtca gctgttagtt tttccatcgc tttctttact
      541 ggggtgtgat tattcgcgac gtgcccttcc tgccccaccc gatctctcac tgttagaccc
      601 gctttgttat gccgcaacga cctggcgaat gaccatcatt gccggccttc ccgttgaata
      661 taacgatcgc tttattcgtg gaattgcggt gttcgcaccg tggcgaaaaa cgcctgggat
      721 ctaccatcag agtcatggtg catgtctggg ccgtcgttcc agaaccatca cggtggtcga
      781 tgaacaaccg caaggtatgg atatggaccc gacctgttca ctgtttacga cagggcaatg
      841 tctgggagaa cccgacctgc tggcatcggc ccgtcgctta caattttttt cacatcagta
      901 ctcgattgct gtgctgatgg caaatgcccg tggtaacagt gcactatggg atgaacatgg
      961 tcgtctcatc gttcgcgccg atcgtggttc attattgtta gtcggtcagc gttcatcaca
     1021 gggttggcaa ggcgatatca ttccattacg ctaggctttt tcggcctgga gcatgccatg
     1081 ttgcgcatta tcgatacaga aacctgcggt ttgcagggag ggatcgttga gattgcctct
     1141 gttgatgtca ttgacgggaa aatcgtcaac cccatgagcc acctggtgca ccccgatcgt
     1201 cctattagtc cacaagcgat ggcgattcat cgcatcaccg aagccatggt cgccgataaa
     1261 ccgtggattg aagatgtgat cccacactat tacggtagtg aatggtatgt cgcgcataac
     1321 gccagctttg accgccgcgt actgcctgag atgcccggtg agtggatttg cactatgaaa
     1381 ctggcccgtc gtttgtggcc agggatcaag tacagcaata tggcgttata taaaacacgg
     1441 aagctcaatg tacagacgcc gccgggcctg catcatcacc gcgcgttgta tgactgttat
     1501 atcaccgccg cgttgcttat cgatattatg aacacctccg gctggacggc agaacagatg
     1561 gccgatatga ccggacgtcc gtcgttgatg acgaccttca cctttggcaa ataccgtggc
     1621 aaagcggttt ccgacgttgc cgaacgcgat ccgggctatc tgcgctggtt gtttaataac
     1681 ctggacagca tgagcccgga gctgcgttta acactgaaac attatctgga aaatacctaa
     1741 tccgcaggcc tagcgggtaa tgttccctgc gccagcgcga ccagaaaagc atattccatc
     1801 gctacgcctt cgtacgattt aaagcgacca gatttaccgc catggcctga gtccatgtcg
     1861 gtacagagca ataaaagatg gttatcggtt ttcagctcgc gcaacttagc gacccatttt
     1921 gccggttccc aatattgcac ctgagaatcg tgtaaaccgg tcgttaccag taaatgtgga
     1981 taagcctgtg cggtgacgtt gtcatacggg ctgtagcttt tcatatactc gtaatattgc
     2041 agatcctgcg gattccccca ctcttcaaac tcaccagtgg taagaggaat tgattcatca
     2101 agcatcgttg ttacaacatc aacaaacggt acctgggcga taacgccgtg gaataattcc
     2161 gggcgttgat taattgcaac gcccatcaac atgccccccg cactcccgcc catcgcataa
     2221 caaagcgaag gagagccata gcccagtttt aacaatgcat cgcaggcatc aagataatca
     2281 ttaaacgtat ttttcttctt cagaaattta ccgtcttcgt accattgttg ccccagctca
     2341 ccgccgccgc gaacatggac aatggcgtag acaaagccac gatctaacaa actcaagcgg
     2401 ctaaaactga aatcggcatc aatacttgcg ccgtaagaac cgtagccata caccagcagc
     2461 gggttgtgtc ctttgcgaaa atgattgcga tggtagacca acgaaaccgg aacttcgaca
     2521 ccatcacggg cgactatcca caggtgttca ctgcggtaat tcgccgcatc aaaaccagga
     2581 acttctgttt gttttaatac acgacgctca ccggtatcca tatccagttc aaacaaagtg
     2641 tctggtgtgg tcatggaaga ataaccataa cgcaatcgcg cggtttcagg ttctggattg
     2701 taggcaatcc aggtcacata ggctggatca tcaaaggcaa taccaatgac ttcccgggtc
     2761 ttgcggttaa tttggcgcaa actggttaac ccgcgctgac gctcttcaac caccagccag
     2821 tcggtaaaca gcgtaaaccc ttccagcatg atgttttcgc gtggcggaat taactcttcc
     2881 cactgttgct catcacgcat acgggtacgg tataagccaa agtttttgcc gtggcggttg
     2941 gaacgcagat aaaaacgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc
     3001 ggcagaaaaa caaacggctc ggcatctgcc atttccgcgt ccagcaggcg aacttcactg
     3061 gtggtggcgc tggccaaatg aatgactaca tagtgctttg aggtcgtttt atgcaggctg
     3121 acgtaatagg tatcgtcttt ttcttcgtaa atcagtttat cttgcgatgc tggcgtaccg
     3181 atggcgtgac gccagacctg ataaggcagc agcgtcaccg gatgcttgcg aacatagtag
     3241 aaagtccagg agtcatttgc ccagacaaag ctgggttcaa cgttatccag cagttccggg
     3301 taccagttac ctgtttccag attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa
     3361 tcttctgcca gcgccataat ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa
     3421 aactcactat gagccgcgcg cttgttggca tcgagcaata tttcccactc gtcccactct
     3481 tcactgaacg ccgattgacg ctggtagata gcatattcac agcctggttc ataaatatgc
     3541 cggtagcggt agccattttt gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg
     3601 atgatttcct ttaagatgcg atcctgcaag gcttgttgtg aggccatcac ccgatgaccg
     3661 taactatttt cttgttgcag gtagtccagg acttccggct gagaacgcgt atcgtcccgc
     3721 agccagtagt aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg
     3781 gcggcttttg gtagcatgtt attgttcttt ctggttgaaa catcttataa gggtggcaaa
     3841 actcaccggg gatgcaagcg aaacagggca gtcattgctt agatgatgac aggtaatggc
     3901 gcgaatatcg aatgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgctgttc
     3961 tggatagccc gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt
     4021 gcgtttttgc tgttcgagat cgcgttcaat gccttcacga acatcctggg ggattttcag
     4081 cgcgtcaccc agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc
     4141 gcagctcagg aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaggcg
     4201 ttgtggatcc agcggttgtt cgattgcctg ctcaatgagt acccgcccct gctcttccac
     4261 gccggcttca cgcaattgct ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc
     4321 acttttagcg gcaaagacta gcgcaaggat caaacgttcc gtacgctcat ccagcggcgt
     4381 actttgcgag ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt
     4441 attccacagc accgtacccg ctaccgctcc gccgccaatc agcaacgcgt tggtgccata
     4501 tttcgtaaga agtttacgtg ctgatttatt tgcgaccagc agtccagcca gtccgcctaa
     4561 tgcgcccggc actaaaagtt tgcccaatcc ctgatccgca gacgaggagg tagaagaact
     4621 gctttgccca agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata
     4681 accattttta gtgcctgtag catagaggat gaggatgtca gaaaatgtat atcgagacaa
     4741 aagatgcgca aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg
     4801 tatttactgt atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt
     4861 ggcattgttt gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt
     4921 gtggtgcgtt ctggggcgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt
     4981 atcagaaccc attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc
     5041 tggtaatgaa tatcggcact tttaccacgt acggttagcg gtactgacca tttatcatct
     5101 ttaccctgaa tgtcctgcaa actgacccat gccacgggat cggcctgacc gacaattttt
     5161 tgatcatctg cccagcgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca
     5221 attccggcag catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta
     5281 ttggcagcga aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt
     5341 tttttcatgt ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag
     5401 gagcgcaagt tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg
     5461 acctgtgggg acggtaattt gccggttttc agaatggtgc tcagcacatc tttttgttct
     5521 gccagccata gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc
     5581 agccaggtgt caagcagatc cgccgtatca agcattttgt cataagcatc ggcttccttt
     5641 ttgctggtaa acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc
     5701 gccatttgca gcctctcata ataactgtga ttctatacag tatatttctt ttcggttgag
     5761 agatcaacac cagcaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt
     5821 aatcagggga tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc
     5881 gtgatgttat taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc
     5941 ggcgtagagg tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat
     6001 cgctcccatg tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa
     6061 aaaccacatt atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt
     6121 gaagaggaag gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc
     6181 gagggtatcc gtcgcctggc ggcagaagag ctgcagctgc ccacttccac ttatcgtttt
     6241 gccgatagcg aaagcctttt ccgcgaggcg gttgccgcca ttggctatcc ctgcattgta
     6301 aaaccggtga tgagctcttc cggcaagggg cagacgttta ttcgctctgc agagcaactt
     6361 gctcaggcat gggaatacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt
     6421 gaaggcgtcg ttaagtttga cttcgaaatt accctgctaa ccgtcagcgc ggtggatggc
     6481 gtccatttct gtgcaccagt aggtcatcgc caggaagatg gcgactactg tgaatcctgg
     6541 caaccacagc aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg
     6601 gtgctggcac tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag
     6661 gtgattttca gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct
     6721 caagatctct cagagtttgc cctgcatgta cgtgccttcc tcggacttcc ggttggcggg
     6781 atccgtcagt atggtcctgc agcttctgcc gttattctgc cacaactgac cagtcagaat
     6841 gtcacgtttg ataatgtgca gaatgccgta ggcgcagatt tgcagattcg tttatttggt
     6901 aagccggaaa ttgatggcag ccgtcgtctg ggggtggcac tggctactgc agagagtgtt
     6961 gttgacgcca ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc
     7021 cggcaaataa acgctacaaa aatgcccgat cctcgatcgg gcattttgac ttttacagct
     7081 tagcgccttc tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca
     7141 gcgcatctgc cggaaccagc caggaaccac cgatgcacag cacgcttttc agcgccaggt
     7201 agtcacggta gttagccgga gaaataccac ccgtcgggca gaaacggacc tgggagaacg
     7261 gacccgcgat cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga
     7321 actctttcaa accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa
     7381 tcagcggaat agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa
     7441 ttgcgaactg tgcacccgct tcagtgactt ctgccagctg ctgtggattc agcaccgtac
     7501 cggcacccac aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac
     7561 actcggtacg cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca
     7621 tcggcaccgc gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca
     7681 ggattgattc tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa
     7741 atttgtcgtc ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac
     7801 gcaaggcgct gaataattca cgtcctgttc ccacgcgtga cgcgctcagg tcaggaatgt
     7861 gtggttcgcg agcagccaat tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc
     7921 cattcacacg aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaagctt
     7981 ctggtgttac gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg
     8041 taactaacgc aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat
     8101 gtaattctgg cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc
     8161 ggtccagcaa acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg
     8221 ctggcgcttc aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc
     8281 ccaggttacc gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag
     8341 cgatcacatt gctgtcgagt gatttttccg ccccttcccg ccagtccagt tcaccattat
     8401 tcagccatgg ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt
     8461 catgcagcag gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct
     8521 ggaagtggtt aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat
     8581 cagaaaggtc agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca
     8641 ccaggtgcat ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca
     8701 ccaccacttt ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca
     8761 tgcgtgtaac ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat
     8821 gaacaaaaga agagcctggc aactgcatcc ccataaactc caccaccatc tggttggtgt
     8881 tggcagtacc gtagaaagta catgttcccg gcgcatggta agacgcggct tctgactcca
     8941 gtaaggccat gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt
     9001 tatttggcaa accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa
     9061 acgacagggc tgccatcgtc agacccggga caatcttgtc gcacaccccg aggaacagag
     9121 caccatcaaa catgttatgg gacagcccga cagccgcaga catcgctatc acttcgcggc
     9181 ttagcagcga caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa
     9241 caccgcccgc aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg
     9301 gatagtgttc ataaggctgg tgagcggaga gcatgtcgtt ataggaggtg atgatggcga
     9361 tattgttacg caacatgctt ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac
     9421 cgtgtgccag gttaccgcat gccaactgcg aacgatgaac ggtcgacgtt ttcgcttgtt
     9481 ctatccgggc gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta
     9541 cgcgtaacaa ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt
     9601 cacaacgcgt ttcattcaga ggatttatga gtgaaacgcc tgtaaccgga gctcataggg
     9661 caaaaacgtt tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg
     9721 aaagtgtaaa aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa
     9781 aggataagcg cagatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg
     9841 ccaccgaggc aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca
     9901 tcgcccacgc ctcagtaatg gagtctaccc atttccaggc ttcttccact tcatcgcgac
     9961 gtacaaacag tgcctgaata ccacgcatgg tttccagcag caaacgttca taggcatccg
    10021 ccagatgcgt ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt
    10081 tatgtttgtg gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag
    10141 gttgcagacg gatagtcagt ttattctgcg gcagatcctg ccacgactct ttaaacagat
    10201 tcagttcagg tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac
    10261 cagtacgcag gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg
    10321 ccacgaaggt ttctgtattg ctgctcttgt tcgcgccctc ttcttccaga tatcccggca
    10381 cttttttgcc ctgggcgaag cccgcagtat attgcccgcg tacggttttt tcgcgtacgt
    10441 tggagcggtc gatgcggcgc agagacttca gtactttcac tttttcatcg cggatgctgt
    10501 ctgcgctcag gtcagacggc ggagacatcg caatcatgca aagaatttgc agcaggtggt
    10561 tctggatcat gtcgcgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc
    10621 ccacttcttc tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca
    10681 caaacaggga gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat
    10741 agtggtcgat acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt
    10801 cctgcgaggt cgccagcgac gtccccagcg gtttctccat gactacgcgt gccggtttag
    10861 cattcagttt tgcctcgcca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg
    10921 caaagtagtt aatggtgata cgattttttt gatccagcat cgcgccgaga cggctgaatg
    10981 cagcagtgtc attgacatcg agattacaaa aatccagacg tgcgctcagg gtgtcccata
    11041 aaccttcatc aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca actttggtat
    11101 atgccgcttt atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct
    11161 gaccggcttt ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt
    11221 tcgcgccgaa aatgaccaag tcacagacct gggctgtttg cgttaccgcc atgtcattct
    11281 ccttaagtta actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca
    11341 ctgtactgct tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg
    11401 taaaagcgcg caaagtgcgg caaaactgat aaaaaaatca tcgttttcca ttgggtaaaa
    11461 atctgacact gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta
    11521 acacagcaca caggcgtaat atctgacaaa actgcatttc gatttctttc agtgcggaaa
    11581 tcgtcattac ccgtgagtct ctttacatca tgaatatgct ggaaaaaatc cagtctcagc
    11641 tggaacattt gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata
    11701 acgcgatcca ttcgagtatt gctgctctgg cactggaagc caatgttagc gaaccgacgg
    11761 tgaatcgttt ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg
    11821 cacagagtct ggcgaatggc actccctatg ttaatcgcaa cgtcaatgaa gatgacagcg
    11881 ttgaatcata cacagggaaa atatttgagt ccgcaatggc aacgctcgat catgtccgtc
    11941 attcactgga taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa
    12001 tcgccttttt cggattaggc tcttcagccg ccgttgccca cgatgcgatg aataagttct
    12061 ttcgttttaa tgttccggtg gtgtactccg atgatattgt gctgcaacgc atgagttgta
    12121 tgaattgtag cgacggagac gtggtggtgc tgatttctca caccggaaga acaaaaaatc
    12181 tggtcgagct ggcgcagctg gcacgcgaaa acgacgctat ggtgattgcc ctcacctctg
    12241 cgggtacccc gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata
    12301 ctgacattta tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg
    12361 cgacaggatt tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg
    12421 aagcgctgaa ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa
    12481 agcgactgtc actgttctaa tcttatacga catccgaatg agattatttt atcgccatcg
    12541 cggcgttatt tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag
    12601 tattacatgt ccagaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca
    12661 gatcgcgata ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac
    12721 ttttctcacg gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc
    12781 gcaaaactgg ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta
    12841 tccaccttta aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc
    12901 aacctgggta aaggtgaagg cgacaaagaa aaagtcggta tcaactacaa aggcctgcct
    12961 gctgacgtcg tgcctggtga catcctgctg ctggacgatg gtcgcgtcca gttaaaagta
    13021 ctggaagttc agggcatgaa agtgttcacc gaagtcaccg tcggtggtcc cctctccaac
    13081 aataaaggta tcaacaaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac
    13141 aaagcagaca ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca
    13201 cgctgtggcg aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg
    13261 aaaattgttg ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac
    13321 atcatcctcg cctctgacgt ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc
    13381 gacccggaac tggtcggcat tcagaaagcg ttgatccgtc gtgcgcgtca gctaaaccga
    13441 gcggtaatca cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt
    13501 gcagaagtca tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct
    13561 gcagaaactg ccgctgggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc
    13621 ctgggtgcgg aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc
    13681 gacaatgtgg aagaagctat tgccatgtca gcaatgtacg cagctaacca cctgaaaggc
    13741 gttacggcga tcattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc
    13801 agctctggtc taccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct
    13861 ctctatcgtg gcgttacgcc ggtgcacttt gatagcgcta atgacggcgt agcagctgcc
    13921 agcgaagcgg ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt
    13981 gtcacccagg gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tattttaacg
    14041 gtagagtaag tacgttgccg gatgcggcga aaacgccaca tccggcctac agttcaatga
    14101 tagttcaaca gatttcgaat attctgaagc aaacttgaac ttatcatcag gcgaaggcct
    14161 ctcctcgcga gaggcttttt tatttgatgg gataaagatc tttgcgctta tacggctgga
    14221 tttcgcccgg tttgcgagtt ttcagcaatt ttagtatcca ggtgtattgt tctggtcgcg
    14281 gaccaacaaa aatctcgact tcttcattca tccgccgcgc aatcgtatga tcatccgcct
    14341 ctaacagatc atccatcggt gggcgcacct gaatcgtcag acgatgcgtc ttgccatcat
    14401 aaatcggaaa cagcggtaca acgcgcgcac ggcacacttt catcaaacga ccaatcgcgg
    14461 gcaacgtcgc tttataggtg gcaaagaaat ccacaaattc gctgtgctct gggccatgat
    14521 cctgatcggg taaataatat ccccagtacc cctgacgtac cgactggatg aatggtttaa
    14581 taccgtcatt tctcgcatgc agacgaccgc caaagcgacg acgcaccgtg ttccagacat
    14641 aatcaaaaac cgggttgccc tgattatgga acatcgctgc cattttctgc ccttgcgagg
    14701 ccatcagcat ggcaggaata tcgacggccc aaccgtgcgg cactagaaag ataactttct
    14761 cgttattacg ccgcatctct tcgatgatct ccagcccttg ccagtcaacg cgcggctgaa
    14821 ttttctccgg cccgcgtatt gccaactcag ccatcattgc catcgcttgc ggcgcggtgg
    14881 caaacatctc atcaacaatc gcttcgcgtt cagcttcact acgttctgga aagcagagcg
    14941 acagattgat taacgcacga cggcgtgagc tttttcccag tcgtccggca atacgtccca
    15001 gccgtgccag aatgggatca cggaactttg gcggcgttaa agcgataccc gccatcgctg
    15061 ctacgcccag ccatgctccc cagtagcgcg ggtggcgaaa ggatttatca aactcaggaa
    15121 tgtattcgct attatttttt ttcgtttcca tgcttttcca gtttcggata aggcaaaaat
    15181 caatctggtg atagtgtagc ggcgcaactt gccccgcacc aaataaaaaa agccggtact
    15241 gactgcgtac cggctgcgaa tggatgttaa ttaatcaaac cgtagctgcg gcacaatctc
    15301 tttggcctgc gccaggaatt cgcgacgatc ggagccggtc agcccttcgg tacgcggcag
    15361 ttttgccgtc agcgggttta cggcctgctg gtttatccat acttcatagt gcagatgcgg
    15421 cccggttgaa cgtccggtat taccggaaag cgcgatacgg tcgccacgtt tcaccttctg
    15481 ccccggtttc accagaatct tgcgcaagtg catataacgc gtggtgtagc tgcgaccatg
    15541 acgaatagcc acataataac ctgctgcgcc actgcgtttg gcaaccacca cttcaccgtc
    15601 acccactgaa agcactggcg taccctgcgg catggcgaaa tcaacacctc tgtgtggtgc
    15661 aacgcgaccg gtcaccggat tagtacgacg cgggttaaag ttagacgaga tacggaactg
    15721 tttcgccgtc gggaatcgca agaatccttt cgccagacca gtaccgttac gatcgtagaa
    15781 tttgccatct tcagcgcgga tagcgtaata atctttacct tctgaacgca aacgtacgcc
    15841 cagcagctgg ctttgctcac gtttaccatc aagcatttct cgagacatta ataccgcaaa
    15901 ttcatcgcct tttttcagtt tgcggaaatc catttgccac tgcatggctt taatcactgc
    15961 gctcacttcg gcgctggtca aaccggcgtt tctggcgctg gcaacaaagc tccccccgac
    16021 ggtacctttc agcagattgt taacccactc tccttgctgc atttcgctgg tcattttaaa
    16081 accgttagcg gcagtacggt cataggttcg ggtttcacga cgagacactt cccaggtgag
    16141 gcgctgcagt tcgccgtccg cggttaatgt ccaggagagt tgttgaccga ttttcaggtt
    16201 acgcaattct ttgtcggccg cagccagttg ggtgatatca cccatatcaa taccatactg
    16261 attgagaatg ctgcttagcg tatcgccagt ggaaacaaca tattcatgca cgcccgcttc
    16321 accggcgatt ttgtcatcca gttcgtcctg gggaatggct tcatcttctt gtgcagcttg
    16381 atcaatcggc tcactggctt caggtaagag cgaacgaatt tcgttctgtt ccagctcaat
    16441 ggttttgaca attggcgtgg catcgcggtg ataaacatag ggccgccaga cagcgacggc
    16501 cagagtaaga acggtgagcg accccaacat aacgcggtgt ggtcgcggta aattattaaa
    16561 cgccagggcg acagagcggg ctatctgttg cacgtaatca cttcctcatt aatctccttt
    16621 caggcagctc gcatactggt tggctaattg actcaggaat tctgaatagc ttgttttacc
    16681 cagtttgata ttcgtcccca agggatccaa cgttcccata cgaacggatg tccctcgtgc
    16741 gacgctctca acgaccgctg gcctgaactg tggctcagca aaaacgcagg ttgctttttg
    16801 ctcaaccaac tgtgttctta tttcatgtaa acgctgcgcg ccaggttgaa tctcagggtt
    16861 aacggtaaaa tgaccaagcg gtgtcagtcc gaactgtttt tcgaaatagc cgtaagcatc
    16921 gtgaaaaacg aaataacctt tccccttgag cggcgcgagc tcgttaccaa cctgcgtttc
    16981 ggttgaggct aattgtgcct caaaatcctt caggttggcg tcaagtttgg ctcgactttg
    17041 cggcataagt tccactaatt ttccatggat tgcaaccgct gtagcccgcg ctatctctgg
    17101 ggaaagccaa agatgcatgt tgaaatcgcc gtgatggtga tcttcgtcac ttttttccgc
    17161 gtggtcgtga tcatcatcat cgccgttaat acttttcatc agtagcggtt tcacatcttc
    17221 aagctgcgca atcgttacct gcttcgcttc tggtaatttg cttaccggtt tttgcataaa
    17281 cgcttccatc tccgggccaa cccaaacgac taagtccgcg ttctgtaagc gttttacatc
    17341 cgatggacgc agtgaataat catgttctga agcgccgtca ggaagtaaaa cctccgtttc
    17401 tgttacccca tcagcaatgg cagaagcgat gaacccaacg ggtttaagcg aagcgacaac
    17461 ggcggcatct gcggcctgtg ttgcaccgcc ccagagagcg gcgaataatg ctgcgaaaag
    17521 aagcgttttt ttatgtaaca taatgcgacc aatcatcgta atgaatatga gaagtgtgat
    17581 attataacat ttcatgacta ctgcaagact aaaattaaca tgacaagtct ggtttccctg
    17641 gaaaatgtct cggtttcttt tggccaacgc cgcgtcctct ctgatgtgtc gctggaactt
    17701 aaacctggaa aaattttgac tttacttggg ccaaatggcg caggtaagtc gacactggta
    17761 cgggtagtgc tcgggctggt aacacccgat gaaggggtta tcaagcgcaa cggaaaactg
    17821 cgcatcggct atgtaccaca gaagctgtat ctcgacacca cgttgccact gaccgtaaac
    17881 cgttttttac gcttacgccc tggtacacat aaagaagata ttttgcctgc actgaaacgt
    17941 gtccaggccg ggcacctgat taacgcaccg atgcaaaagc tctctggtgg cgaaacgcag
    18001 cgtgtactgt tagcgcgagc attgttaaat cgcccgcaat tattagtgct ggatgaaccc
    18061 actcaaggcg tggatgtaaa tggccaggtg gcgttatatg accttattga ccaactgcgt
    18121 cgtgaactgg attgtggcgt tttaatggtt tctcacgatc ttcatctggt gatggcaaaa
    18181 accgatgaag tgctgtgcct gaatcatcac atttgttgtt ccggcacacc ggaagttgtt
    18241 tccctgcatc cggagtttat ttcaatgttt ggtcctcgtg gtgctgaaca actgggtatc
    18301 tatcgccatc atcataatca tcgtcacgat ttacagggac gaattgtttt gcgtcgggga
    18361 aatgatcgct catgattgaa ttattatttc ccggttggtt agccgggatc atgctcgcct
    18421 gtgccgcggg tccgctgggt tcgtttgtag tctggcgtcg tatgtcttat ttcggtgata
    18481 cgctggctca tgcctcatta cttggtgtcg cgtttggttt gttgctggac gtgaatccat
    18541 tctatgcggt gattgccgtt acgctgctgc tggcgggcgg tctggtatgg ctggagaagc
    18601 gtccacagct ggcgatcgac acgttattag ggattatggc gcacagtgcc ctgtcgctgg
    18661 gcctggtggt cgttagtctg atgtctaaca ttcgtgttga tttgatggct tacctgttcg
    18721 gtgatttact ggcagtgacg ccagaagatc tcatctctat tgcaattggc gtggtcatcg
    18781 tggtggctat tttgttctgg caatggcgca atttgctgtc gatgacgatt agcccggatc
    18841 tggcgtttgt tgatggtgtg aaattacagc gagtgaaatt gttgttgatg ctggtgacgg
    18901 cattgacgat tggtgtagcg atgaaatttg tcggtgcgtt gattattact tcgttgctga
    18961 ttattcctgc agctactgcg cgtcgctttg cccgtacgcc ggaacagatg gctggtgtcg
    19021 ctgttttggt ggggatggtg gcagtaactg acggtttaac cttttccgcg ttttacgata
    19081 cgccggcggg tccgtcggtg gtgctatgtg cggcactgtt atttattatc agtatgatga
    19141 aaaagcaggc cagctaatct atcgctgaac acatttgtcg gatgcggcgc gagcgcctta
    19201 tccgacctac ggttcgctat ctctggtagg cctggtaaga cgcgaacagc gtcgcatcag
    19261 gcacactgcc aatgtcggat gcggcgcgag cgccttttcc gacctacggt tcgctatctc
    19321 tggtatatgc ctgataagac gcgaatagcg tcgcatcagg cacactgcca atgtcggatg
    19381 cggtgcgagc gaccaatccg acttacggca tttctggcgg cgttatgcca aagtgattcc
    19441 acgcccgcgt cgtcgccata cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca
    19501 aataaggttc cagcacatcc tcaatggttt cacgttcttc gccaatggct gccgcaaggt
    19561 tatccagacc taccggccca ccaaagaact tatcgattac cgccagcaac aatttgcggt
    19621 ccatataatc gaaaccttca gcatcgacat tcaacatatc cagcgcctga gcagcgatat
    19681 ctgccgagat ggtgccatcg tgcttcactt cggcgaaatc acgcactcga cgcagcagac
    19741 ggttggcaat gcgcggcgta ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac
    19801 tcatctcaag ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct
    19861 gataaaactc cagacgttgc acaataccaa aacggtcgcg caacggtgat gtcagcgaac
    19921 ctgcgcgcgt ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg
    19981 ccgccggacc ttcaccaatc atgatatcca gttggtagtc ttccattgcc gggtacagca
    20041 cttcttcaac aactggcgat aaacggtgga tctcatcaat aaacagcacg tcatgcggtt
    20101 caaggttagt gagcatcgca gccaaatcgc ccgccttttc cagcaccgga ccagaagtcg
    20161 tgcgtaaatt aacgcccatt tcattggcga caatgttggc aagcgtagtt ttacccaacc
    20221 ccggaggacc aaaaatcaac aaatgatcga gggcatcgcc gcgcagtttc gctgctttga
    20281 tgaaaatctc catctgtgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag
    20341 ggcgaatggc gcgatctgct acatcttccg gcaaagtggt accggcagaa atcagacggt
    20401 ctgcttcaat catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc
    20461 actgctggcg tcagggcgag cgattttgct caccatgcgg cttgcttctt gtggtttata
    20521 gcccagcgcc accagcgcgg caaccgcttc ttgttcagca tcgtcggtcg ccgggctggc
    20581 aggagacgtg agtaccaggt cggcggctgg cgtaaagaga tcgccatgca aacctttaaa
    20641 tcggtctttc atttcaacaa tcaagcgttc ggcggttttt ttgccaatac ccggcagttt
    20701 caccagtgcc cccacttctt cacgctcaac ggcattaacg aactgctgcg ctgacattcc
    20761 ggagaggatc gccagcgcca acttcgggcc gacgccgttg gttttgatca actctttgaa
    20821 caacgtgcgc tcttgtttat tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac
    20881 aaagtgggtg aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat
    20941 cggcatatgc acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt
    21001 ttcaatgatg at
//
LOCUS       DBFIFS010000059        20877 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.059, whole genome shotgun sequence.
ACCESSION   DBFIFS010000059 DBFIFS010000000
VERSION     DBFIFS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.059"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(102..1751)
                     /gene="actP"
                     /locus_tag="N7A07_002042"
     CDS             complement(102..1751)
                     /gene="actP"
                     /locus_tag="N7A07_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU2476708.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1748..2062)
                     /gene="yjcH"
                     /locus_tag="N7A07_002043"
     CDS             complement(1748..2062)
                     /gene="yjcH"
                     /locus_tag="N7A07_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU2476709.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2263..4221)
                     /gene="acs"
                     /locus_tag="N7A07_002044"
     CDS             complement(2263..4221)
                     /gene="acs"
                     /locus_tag="N7A07_002044"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU2476710.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4614..6050
                     /gene="nrfA"
                     /locus_tag="N7A07_002045"
     CDS             4614..6050
                     /gene="nrfA"
                     /locus_tag="N7A07_002045"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU2476711.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSHDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6095..6661
                     /gene="nrfB"
                     /locus_tag="N7A07_002046"
     CDS             6095..6661
                     /gene="nrfB"
                     /locus_tag="N7A07_002046"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU2476712.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6658..7329
                     /gene="nrfC"
                     /locus_tag="N7A07_002047"
     CDS             6658..7329
                     /gene="nrfC"
                     /locus_tag="N7A07_002047"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU2476713.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7326..8282
                     /gene="nrfD"
                     /locus_tag="N7A07_002048"
     CDS             7326..8282
                     /gene="nrfD"
                     /locus_tag="N7A07_002048"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU2476714.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8302..10020
                     /gene="nrfE"
                     /locus_tag="N7A07_002049"
     CDS             8302..10020
                     /gene="nrfE"
                     /locus_tag="N7A07_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU2476715.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            10013..10396
                     /gene="nrfF"
                     /locus_tag="N7A07_002050"
     CDS             10013..10396
                     /gene="nrfF"
                     /locus_tag="N7A07_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU2476716.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10393..10989
                     /gene="nrfG"
                     /locus_tag="N7A07_002051"
     CDS             10393..10989
                     /gene="nrfG"
                     /locus_tag="N7A07_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU2476717.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11331..12644
                     /gene="gltP"
                     /locus_tag="N7A07_002052"
     CDS             11331..12644
                     /gene="gltP"
                     /locus_tag="N7A07_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU2476718.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12822..13511)
                     /gene="yjcO"
                     /locus_tag="N7A07_002053"
     CDS             complement(12822..13511)
                     /gene="yjcO"
                     /locus_tag="N7A07_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU2476719.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13605..15752)
                     /gene="fdhF"
                     /locus_tag="N7A07_002054"
     CDS             complement(13605..15752)
                     /gene="fdhF"
                     /locus_tag="N7A07_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15333..15335),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HGU2476720.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15950..17416)
                     /gene="mdtP"
                     /locus_tag="N7A07_002055"
     CDS             complement(15950..17416)
                     /gene="mdtP"
                     /locus_tag="N7A07_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU2476721.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17413..19464)
                     /gene="mdtO"
                     /locus_tag="N7A07_002056"
     CDS             complement(17413..19464)
                     /gene="mdtO"
                     /locus_tag="N7A07_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU2476722.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19464..20495)
                     /gene="mdtN"
                     /locus_tag="N7A07_002057"
     CDS             complement(19464..20495)
                     /gene="mdtN"
                     /locus_tag="N7A07_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU2476723.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20514..20789)
                     /gene="ytcA"
                     /locus_tag="N7A07_002058"
     CDS             complement(20514..20789)
                     /gene="ytcA"
                     /locus_tag="N7A07_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU2476724.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4706 a         5510 c         5708 g         4953 t
ORIGIN      
        1 ttttttttta atgagtttat ttgtttaata ttatgggaaa aggtgatgca tttgggagag
       61 gaagagtatt ccccggtcag acgaccgggg aaggggagag attaatgcgc gcggccttgc
      121 tcaacgccaa agccggtctg ggagcggata aactgcgcgc ggaacagttc acgctcacgc
      181 gcgccttccg ctgagttatc ggttgccgag aagaaccaga tgccgaggaa tgccacggtg
      241 atagagaaca gcgccgggta ttcatacggg aagatggctt tttcgtgacc gaggatctgt
      301 acccaaatcg tcgggccgag gatcatcagt accactgcgg taatcagccc cagccagcca
      361 cccatcatcg cgccacgcgt ggtcagtttc gaccagtaca tagaaagcag aatgatcggg
      421 aagttacagc tcgccgcgat ggcaaacgcc agccccacca taaaggcgat gttctgattc
      481 tcaaacagca cgccgaggat aatcgcaatc acacccaaaa tcaggacggt gattttcgat
      541 acccgcagct cttcacgttc ggtcgcgcct tttttgaaga cgttagcgta caggtcatgc
      601 gaaaccgctg atgcgcccgc cagcgtcaga cccgcaacca ccgcgaggat agtggcgaaa
      661 gcaaccgctg aaataaagcc gaggaacagg ttgccgccca ccgcattggc taggtgaact
      721 gctgccatgt tgttaccacc aatcagatgg cccgccgcgt ctttatattc cggattcgca
      781 ccgaccagca tgatcgcgcc gaagccgata ataaaggtca ggatatagaa gtagcccata
      841 aatccggtag cgtagaacac gcttttacgc gcttcgcggg catcgctgac cgtaaagaag
      901 cgcatcagaa tgtgcggcaa gccagccgta ccaaacatca gtcctagccc cagagagagt
      961 gcggagatcg gatctttcac cagtccgccc ggtttcatga tgtcgacacc tttcgggtgt
     1021 accgccatcg cttcgctgaa cagattgttg aagctaaagc cgacgtgttt catcaccata
     1081 aaggccataa agctggcacc gaacagcaac agcacagctt tgataatctg tacccaggtg
     1141 gtcgccagca tgccgccgaa caggacgtac atcatcatca gcacgccgac cagcaccact
     1201 gcaatgtgat agttaaggcc aaacagcagc tcgatcagtt tacctgcccc caccatctgg
     1261 gcgataaggt aaagcgccac caccaccaga gaaccacagg ccgaaagaat acgaatcggc
     1321 ccttgtttca gacgataaga ggctacatcg gcaaaggtgt aacgccccag gttacgcaga
     1381 cgttcggcaa tcaggaacaa aatgatcggc cagcccacca ggaagcccag cgagtaaatt
     1441 aagccgtcat agccggaggt aaacaccagt gcggagatcc ccaaaaatga ggcggcggac
     1501 atatagtcac cggcaatcgc cagcccgttc tgaaaaccgg tgatattgcc gcctgcggtg
     1561 taatagtcat tacgcgaacg tacgcgtttt gacgcccagt aggtaatgcc gagcgtaaac
     1621 acgacgaaaa tcaggaacat aataatcgcc tgccagttcg ttggctggcg ctctacggcc
     1681 ccgctaatag catccgcggc gttagctgcg aaagggagtg tggcggcaag cgccgtcaga
     1741 actcttttca tgatgcttgt acctcatgca ggacttcatt attaagacgg tcgaattcgc
     1801 cgttcgcccg ccagatgtag ataccagtga gaacaaagga gatcactatt accccaacac
     1861 cgactggaat accgcgtgtg acgctggtgt tcggattcag cggggtgccc agccagccgg
     1921 gcgcgaaggc gatcagtaaa ataaagccga tataaactgc cagcataata atcgacagga
     1981 tggtggcaaa ccgttgccgt ttttcgacta actccctgaa atgcgcattg tcttctatcc
     2041 gctgataaat agtgccattc atcacagatt ctccagaggt aatgtaggga ttgttttaat
     2101 tcccgctccc ttatgggaga aggttaacgc tcgggtaacc cttgccgaat gtaggccgga
     2161 taaggcgtta tacgccgcat ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc
     2221 aggcctacaa accgttaccg actcgcatcc ggcaattgtg ggttacgatg gcatcgcgat
     2281 agcctgcttc tcttcaagca gcttctcgac tacgccagga tcggcaagcg tcgaggtatc
     2341 gcccaggttg ctggtatcgc ccgccgcaat tttgcgcaga atacggcgca taattttgcc
     2401 ggagcgggtt ttaggcaggg agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc
     2461 aatctcttta cgcacccagt tgcggacttc tgcgtacagt tctggtgacg gttcctcccc
     2521 gtgattaagc gtgacgtagg cgtagatcgc ctgaccttta atattgtgcg gaataccgac
     2581 gacagcggct tcggcgattt tcggatgcgc caccagcgcc gactcaatct ccgccgttcc
     2641 cagacggtga ccggagacgt tcagcacatc gtccacacgc ccggtgatcc agtaatagct
     2701 atcttcatca cgacgcgcgc cgtcgccgct gaaatacata tttttgaagg tggaaaaata
     2761 ggtctgctca aaacgttcgt gatcgccaaa cagcgtacgc gcctgacccg gccaggagtc
     2821 ggtgatcacc aggctacctt cggtagcccc ttccagcggg ttaccttcgt tatcgaccag
     2881 cgccggttgc acgccgaaga acggacgtgt tgccgaaccg gctttcagct cggtagcgcc
     2941 aggcagcggg gtgatcatga aaccgccggt ttcggtctgc caccaggtat cgaccaccgg
     3001 acatttctcg ttgccgattt ttttccagta ccactcccac gcttccgggt taattggctc
     3061 gcccacggaa ccgagaatgc gcagcgacga acggtcggtg ccttcgatcg ctttatcgcc
     3121 ttccgccatc agcgcgcgaa tcgccgtggg cgcggtatag agaatattga cctgatgctt
     3181 gtccaccacc tgtgccatac gggcaggcgt cggccagttc ggtacgcctt caaacatcag
     3241 cgtggtcgcg ccgcaggcca gcgggccgta cagcaaataa ctgtgtccgg tcacccagcc
     3301 cacatcggcg gtgcaccagt agatatcgcc cggatgataa tcaaagacat atttaaaggt
     3361 cagcgccgcg tacaccagat aaccgccggt agtgtgcagt acgccttttg gttttccggt
     3421 agaaccggag gtatagagaa taaacagcgg atcttcggcg ttcatctctt ccgcctggtg
     3481 ctgatcgctg gcttgctcaa cctggtcgtg ccaccacagg tcgcgccctt cctgccagtc
     3541 aattttcccg ccagtacgct tcagtaccac cacatgctct acgctggtga cgttcgggtt
     3601 ttttagtgcg tcatcaacgt ttttcttcag cggaatacta cgcccggcgc gcacgccttc
     3661 gtcggaagtg atcaccagtc gtgagttgga atcgataatg cgcccggcaa ccgcttccgg
     3721 cgagaagcca ccgaaaatta ccgaatgcac cgcgccaata cgggcgcagg ccagcatcgc
     3781 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3841 cagcttgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3901 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3961 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     4021 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtacg gtttgatcca
     4081 gtcgagaatt tttccctgtt cgccccagaa ggtatcaggt gcgttaatag attgttgata
     4141 catcgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4201 gtgtttgtga atttgactca tgcttttgtt ctccttgtag gatgttaata atatgtggca
     4261 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4321 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4381 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4441 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4501 ttatttgtta cacttagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4561 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4621 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4681 ctgttcacgc tgaacaaacg gctgctcccg caaaacctgt aactgtggaa gcgaagaatg
     4741 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4801 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4861 ccttctcgca cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4921 aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg tccgctaccg atggcgtgct
     4981 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     5041 tccacggtaa atgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgatt
     5101 gccataacac cgcctctcca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5161 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5221 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5281 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5341 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5401 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5461 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5521 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5581 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5641 ttgaagatca actggttcac gctcacttcg aagcgaaagc ggcgctggac gcaggtgcga
     5701 cggaagccga aatgaagccg attcaggacg atatccgtca tgcccagtgg cgttgggatc
     5761 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggtctg aggatgctcg
     5821 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacta
     5881 aaggcatcac ccatgaaatc cagatcccgg atatctcgac caaagagaaa gcccagcagg
     5941 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     6001 tcccgcagtg ggaagagcag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     6061 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6121 tgccggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6181 ggcgcaggca tctgatgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6241 tctggactgt cataagccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6301 cccgaataac aaactgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6361 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg ttggagagca
     6421 gaacagcgtc tgtatgtcct gccatctgcc ggaacagttg caaaaagcgt tctggccgca
     6481 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6541 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6601 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6661 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6721 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6781 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag
     6841 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6901 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6961 gacgtctgcc cgaccggtgc atcttttcgc gatgctgcca gtgggattgt cgatgttaat
     7021 ccggatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     7081 attcatccgg tcacgaagac agcggacaaa tgcgatttct gccgtaagac caatttgcag
     7141 gccggtaagc ttcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7201 ctggacgatc ccaacagtga gatttcgcaa ctgctgcgcc agaagcccac ttaccgctac
     7261 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7321 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7381 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7441 gtcgcttcta cccgcaggcg ggcggtgcag acagcacgtt gctgcgcacc acgctgattg
     7501 tcggacctgg cgcggtgatc ctcggtctgt tgagcctcgt cttccacctg acaagaccgt
     7561 ggaccttctg gaagctgatg ttccactaca gttttacttc ggtgatgtcg atgggggtga
     7621 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7681 atttgcttgc cctgcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7741 gcctgctgac gcccgttcat cgcggactgg aaacattgat gctggtgctg gcggtgttgt
     7801 tgggggctta taccggtttt ctgctgtcgg cgctgaaatc gtatccgttc cttaataacc
     7861 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcactga
     7921 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcag tttgtacacc
     7981 gtatggaaat ccccgtggta tggggtgaaa tcttcctgct ggcggcgttt tttgtcggtc
     8041 tggcgctggg cgatgacggt aaagtgcgtg cgctggtggc ggcattgggc ggcggtttct
     8101 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgttca
     8161 aaccgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8221 tggtcggcgt gttgatgttg cgatttttca ttctctacgc cgggcagtta acggtggcgt
     8281 aagccagaac agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgctg
     8341 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8401 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcacagtt cgcgctcctg
     8461 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8521 gtcgcccaac atagctacag cttgctgtcg tggaaactca aactggcggc ggtgtggggc
     8581 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8641 gcctggcaat atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8701 ctcatgctcg ccgcactgct actgtttgta gtgctgtggt ccgatccctt cgtgcggata
     8761 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8821 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8881 ctggcgagtc tactgcgcgg cgagtttgat ggtgcctgcg cccgaatttg ctggcgctgg
     8941 gcgttacctg gctggagtgc attaactgcg gggatcatcc tcggttcctg gtgggcctac
     9001 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaacgc ctctttatta
     9061 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9121 tttcgccact ggtcgctgtt gttggcgata gttactctga tgctgtcgct gctgggcacc
     9181 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgctctgga taacgttcgc
     9241 gctgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9301 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9361 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9421 tatccgatga tttacggcct gctgggctgg ggacgtctct ccgttggcgc accgtatttt
     9481 aaccgcgcga cgttaccgtt tggtctgttg atgttggtgg tgattgtgct ggcgacgttt
     9541 gtctctggca aacgcgcgca gcttccggcg ctgctggcgc atgcgggcat actgttattt
     9601 gccgcaggga tcgttgtctc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9661 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9721 aaaggcaatt acaccagcga aaaagcgata gtggcgctgt ttgaccatca gcaacgcatt
     9781 ggtgaattaa cgccggagcg gcgtttttac gaagctcgtc gtcagcaaat gatggaaccg
     9841 tcaattcgct ggaacggcat ccatgactgg tatgcggtca tgggggagaa aactggagcg
     9901 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9961 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaagaagcg cgatgtataa
    10021 agggcttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg ttgtagacac
    10081 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10141 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10201 gcgccatcag gtttacagca tggtggcgga ggggaaaagt gaagtcgaaa tcattggctg
    10261 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10321 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10381 gagggcgaag cgatgaaaca gcctcaaata ccggtgaaaa tgctgacaac actcacgata
    10441 ttgatggtat tcctctgtat cggtagttat ctgttaagcc caaaatggca ggcggtacgt
    10501 gcggagtatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaacccggaa
    10561 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacagtgg
    10621 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10681 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct ggcgacggtg
    10741 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10801 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10861 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10921 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10981 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    11041 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    11101 ttccataaca aatggttatt cattaatcct gccataaact cgcgttatta tgcattaatg
    11161 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11221 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgccctggca gtatgtcacg
    11281 ttctcgcgtt tctgaacggg gaacggcgct ccattgagga agtcattcat atgaaaaata
    11341 taaaattcag cctggcctgg cagattctgt ttgctatggt gctgggcatt ctcctgggaa
    11401 gctacctgca ctaccatagc gacagccgcg actggctggt cgtcaatttg ctctctccgg
    11461 cgggtgatat cttcatccat ctgatcaaaa tgattgttgt gccgattgtg atctccacgc
    11521 tggtggtggg tatcgcgggt gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa
    11581 ccattatcta cttcgaggtg atcaccaccg ttgccatcat tttggggatc actctggcga
    11641 acgtcttcca gcccggtgcc ggggtggata tgtcgcagct ggcgaccgtc gatatctcga
    11701 aatatcagag cactacggaa gcggtacaaa gcagttccca cggcattatg ggcacgattt
    11761 tgtcgctggt gccgacgaat attgtggcgt cgatggcgaa aggcgaaatg ttgccgatca
    11821 tcttcttctc ggtgctgttt ggtctggggc tttcctccct gcccgcgacg catcgtgaac
    11881 cgctggtgac cgtgttccgc tccatctctg aaaccatgtt taaagtgact cacatggtga
    11941 tgcgttatgc gccggtgggt gtgtttgcgc tgattgcagt gacggtggct aactttggtt
    12001 tctcttctct gtggccactg gcgaaactgg tgctgctggt gcatttcgcc attctgttct
    12061 tcgcgctggt agtgctggga attgtggcgc gcctatgcgg gttaagcgtc tggatcctga
    12121 ttcgtattct gaaagatgag ctgattctgg cgtactccac tgccagctct gaaagcgtac
    12181 tgccgcgaat tattgagaag atggaagcct acggagcacc ggcgtcgatc accagcttcg
    12241 tggtgccgac cggttactct tttaaccttg atggttcgac gctgtatcaa agtattgccg
    12301 ctattttcat cgctcagctg tatggcattg acctgtccat ctggcaggaa atcattctgg
    12361 tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt gccgggcgtg tcgtttgtgg
    12421 tgttgctggc aacgctgggt agcgtaggta ttccgctgga aggtctggcg tttattgctg
    12481 gtgttgaccg tatcctcgac atggcgcgta ctgcactgaa cgtggtgggt aatgcgctgg
    12541 cggtgctggt gattgccaag tgggaacaca aatttgaccg caaaaaggcg ctggcttatg
    12601 agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca ataattgaag attgccgggg
    12661 atatcccccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12721 tcagacagcg ctagcgtgat tttgtaggtc tgataaggcg tttacgctgc atccgacatc
    12781 aacgcctgat gcgacgctta acgcgtctta tcaggcctac cttaaccgtt agttaatttt
    12841 tcaaactctt cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc
    12901 gccttttgct tgttcttctc gataaaacct tcttcaccgt ttaagaacat cattcccgcc
    12961 cagtactcgg aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc
    13021 ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg
    13081 gcatcgacgg cagagtcgtt ctccagatct tccgaggcgt tttctaacag cgaaatcgcc
    13141 tttggataat ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca
    13201 ccttctttac tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc
    13261 agactgaccg gattggtgat tttgatctgc gccaacagcg cgcaggcatc agcatcaccg
    13321 ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag
    13381 ctgtcggcaa gaaaatattg tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg
    13441 ctgccaggct cggagtcgtt ggcgtgggca aagaatgtca aaaacaacat caatgcgata
    13501 atttttttca ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg
    13561 gataaaaaaa cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct
    13621 tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc
    13681 tgatcggcga tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc
    13741 gggcttaagt tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg
    13801 taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta
    13861 cgcgagtgca cccaaaccaa tgcctcatct tcaatactca gacgtttggc gtcttcggta
    13921 ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc
    13981 atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg
    14041 tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc
    14101 ggggtgtcaa acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg
    14161 cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa
    14221 tccgggcaca gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga
    14281 taacccatac gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc
    14341 ggttcaactg ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca
    14401 tgctcgcccc acgacgttga cggtaaaata acatccgccg ccgacgcggt tttggtcata
    14461 aagatgtcct gaacgataac cagttccaga tcttcaaagg ctttacgtac tgccgacagc
    14521 tccgcgtcag tttgtagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc
    14581 gctgcgcggt gcggcagctc gctgatgcga taaccggtat gagcaggcag gctttccacg
    14641 ccccaggctt tggcgaattt ctcgcggtta gccggatctt ttacgtactg gtatcccgga
    14701 tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc
    14761 gggttaacac ccgcatgtgg cttaccgagg ttaccggtca gcatcgcgag gctggtcaga
    14821 gaacgcacgg tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg
    14881 gcgcttttcg cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacgccg
    14941 gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag
    15001 ccttctgtac gggaagcgac gaatgctttg tcgtacagat tttcttcaat aatgacatgg
    15061 cccatcgcat tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca
    15121 atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta
    15181 gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg
    15241 aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc
    15301 gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta
    15361 ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta
    15421 ccacgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc
    15481 aggcgctcgg caacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca
    15541 cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg
    15601 aagtcccagc cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct
    15661 gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg
    15721 caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg
    15781 catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc
    15841 ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt
    15901 gacgtgacga aacgaaatac cgcgtgacag ccatcacacg gcagacattt tattttttct
    15961 cgacgacggg agccgcctga tacccgctgc ccagcgattt catcaactga atgctttgga
    16021 tcacccggcg gctgtccagc atcagtaatg acatctcttc ggcaagcacc ggcaaccggg
    16081 cttcggtagc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct
    16141 gggtgaagcg cgttgcttcc acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt
    16201 gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac
    16261 gttcaatcat catgttgctg gcggcgcgag tgccttcgag attggcattc aaccgtccac
    16321 cgtcaaacag cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga
    16381 ataaggtatc caggtggatg gagtccagac cgaaaaacgc tttgatatca aagcttgggt
    16441 agaacagcgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg
    16501 cttgcagatc cgggcgtctg gcgagcaact cataagagag tgtcgccgga ataccggtct
    16561 ggactcgtgg taatgccact ggtttgatct ccggcatatc gctggctccc gcgccaatca
    16621 atgcacgcag agattctcgc gtttcggtga tttgcccttt aacggcagca atttgtttat
    16681 cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgcgcttcc agaccgtgcg
    16741 ccactttact ttggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca
    16801 gatcgagcat ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca
    16861 gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg
    16921 taaccgctga gcgatgcaca ccccacaaat caagatccag tccggcaaac aaaccgactg
    16981 tggcttccgt atagtacggc ccgtccatac ccagtgctgg cgcatccatc gcataagggc
    17041 ttaaaaagcc gttcgccgag acgcgttggc ggttgagcat ccctaacgct gcgacctgta
    17101 attgtgaacc ggcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg
    17161 cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat cagcgcatcc agctgcgggt
    17221 cattgagttg tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat
    17281 cggccagttt gatttgttcc ggtttgagct gttgatgagg cgcagaatcc ttgcgtacca
    17341 gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa
    17401 gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg
    17461 ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa
    17521 ggtgggcagg atggcctgcg aggcgggtgt ctcttcaagc gttatttgag gagaacggct
    17581 gagtgcggtt gccagaccgg cagcgtattt ttccagggcg tcggcaaact gacctgcttt
    17641 gtccggctgt agtgcgttat ccagcgcctg cgccgagttc caggtggcat cccaggcgtg
    17701 aagaagatcg cggccctgat gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc
    17761 ttcgctgtcc agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa
    17821 cgccgcatgc agacctatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg
    17881 gcgtgggatc cgcattactt tacttagcat acccagcgcg ccagccagtt tttgcggcag
    17941 cgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc accgcagaca ccacggtacc
    18001 aatgatgata cccagggcgc gatcgcgaat ttccaccagg tcgtacaccg gaccaaaaac
    18061 gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg
    18121 ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa
    18181 ttcgacaata ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc
    18241 gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgatgaac cgacattcgg
    18301 attagcgacg atcacgcagg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct
    18361 gtagaaggtg taacagatca aacaggcgag cagcgttttt accgcgtagc gcatatagtc
    18421 tggattggta aaagcatcag cgaccattga tggcggtttg gcggcgggcg ttggcggcgt
    18481 attcgggtcc atctgaccca gttgtaacaa cgtctggcag atattctcca ggttacattc
    18541 ccgtgccgcc atcgcttcac tttcactgat ccgccagtcg ctttgccagc actgaccttc
    18601 ggtaatggca tgctgcagct tgttgatttc tgaagctaat ttttgtcgga attcaataat
    18661 tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt
    18721 taccgttgcc acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc
    18781 gaggcaaaag acattgagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg
    18841 tagcggtgcg aggctgtccg tcaggtggct aatggcatca tcaagccgat cattaagcgc
    18901 ttgatgcatt tgcgaaatgg cacgactggg aaaccacagt acgccgatta acgtcattaa
    18961 taacgtcggg tagaggccaa caacgataca ccacagcgtt aagcgcacga ccacttccgg
    19021 atagtcgagc atggcgggga aggtttgccc ataaatggcg acaatggcga cagcgaaaaa
    19081 gaccagtccc aaccgatggg tgcgcatcaa aaacatgcag cccatcagga tcggcccggc
    19141 gatgatcaag cggatcaacg gttcgccgta tgaccatttg tagatcaaaa acaggctgcc
    19201 gatctccagc accgtggcaa ccacaaacaa gatcgcgaca aatttggtgt aaaacgcgtt
    19261 cgactgaata ccgtaaaaca gcactgccag cgataacgcc acaaaaggga tctcaaaagt
    19321 catcgagatc agaatcacca gcaggcagcc tacccagagt tgcacggtct gcggcacgcg
    19381 acctggtcgc cgctcgctta actcttcatg aaagaacgcc agcagcctga ccaccggtaa
    19441 tggcagggag ttgagcgcgc tcattattgc ggctcaagat tagcgactgc cgaagcgccg
    19501 atgcggaaca tttccgggtc aggtttatca accatgattt tgaccggaaa acgctgggca
    19561 acacggaccc agttaataga acgagaaact ttcggcaggc cgcccagcac caggccgccg
    19621 tcatccggta gcacgccgta gccaattgaa tccactttac cctcgaaagt tttgccgctg
    19681 tcactcatca ggcgaatcgt tgcaggtgta cctgagcgaa tatttttcag atcggtttcg
    19741 cggaagttgg cgatcacgta ccagtgacga gtgtcgatta gggtaaaaat agggcgcatg
    19801 gcagaagcaa attgcccgac ggaggttttg agggaaatga cccggccatc aaacggtgcg
    19861 cgaacggtcg ccatttccag atgcagtttg gtcagggcaa tatccgcttc gaccgccgca
    19921 cgctgggcaa ctaatgcatc cacgccgctg acggcgctgg cggctgactg cgcctgcaac
    19981 aatacagcat taagatcggc ttctgcagcg cgctgcgcgg tcctcgcacg gtcaacgtct
    20041 tccgctgaga caaaaccttc tttcagtaat ggctcggtgc ggcgtaatgt atctgtggcc
    20101 tgtttagcgg cggcacgggc tttttctacc gtggcgttaa ccgagtcggc accaaactgt
    20161 tgcgcgtcaa cgctacgctg ggtgagcata atttgcttat ccagtgccgc gagggaggct
    20221 tcagatttcg ccagattggc ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc
    20281 tgtttgactg cctggttgtc ggtgaccgcc agttctacaa tgcggccgct gacttccggc
    20341 accacatcaa tggtatctgc tgacacgtaa gcgtcattag ttgatggcgc actgtctacg
    20401 cgccagataa cgaaaacaag ggcaaccagc gccaacgcaa ccactaacag agcagggaat
    20461 ttactgcgag gagctttttt cggcgtactt tccatgagcg cgtctcaaat agattagaaa
    20521 aatgccagcc agaacagcat ggcgtagaga gcaaaaaggg cggtataaat aattccgaca
    20581 aatgccagct tgatatttgc ccgctgaata actcgcctcg tgatgagcgt caatataagg
    20641 ctggcaatgg cgcagaaaaa ccagctggga taataagcgc cgatcaccgg gattgccgga
    20701 gataatgagc atcccgaaac catgaagacg ggaattatcc aggctgtttt tttgagttgc
    20761 atcgccatgc gagagagaac tgttggcata ttaaatacat cctgtctgct gcgaacaata
    20821 tctgtagtga atatcaatat ccattgaatg cgtcagtatg gctattttct tgatgaa
//
LOCUS       DBFIFS010000060        20670 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.060, whole genome shotgun sequence.
ACCESSION   DBFIFS010000060 DBFIFS010000000
VERSION     DBFIFS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.060"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            3..1187
                     /gene="proV"
                     /locus_tag="N7A07_002059"
     CDS             3..1187
                     /gene="proV"
                     /locus_tag="N7A07_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU2476725.1"
                     /translation="MKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLA
                     IEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKI
                     AMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPNELS
                     GGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHD
                     LDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSAKDIARR
                     TPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLD
                     AALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVN
                     NG"
     gene            1180..2244
                     /gene="proW"
                     /locus_tag="N7A07_002060"
     CDS             1180..2244
                     /gene="proW"
                     /locus_tag="N7A07_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU2476726.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2301..3293
                     /gene="proX"
                     /locus_tag="N7A07_002061"
     CDS             2301..3293
                     /gene="proX"
                     /locus_tag="N7A07_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU2476727.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3485..4669
                     /locus_tag="N7A07_002062"
     CDS             3485..4669
                     /locus_tag="N7A07_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476728.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4793..5530
                     /gene="ygaZ"
                     /locus_tag="N7A07_002063"
     CDS             4793..5530
                     /gene="ygaZ"
                     /locus_tag="N7A07_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU2476729.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5520..5855
                     /gene="ygaH"
                     /locus_tag="N7A07_002064"
     CDS             5520..5855
                     /gene="ygaH"
                     /locus_tag="N7A07_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU2476730.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5946..6476
                     /gene="emrR"
                     /locus_tag="N7A07_002065"
     CDS             5946..6476
                     /gene="emrR"
                     /locus_tag="N7A07_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU2476731.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDHLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6603..7775
                     /gene="emrA"
                     /locus_tag="N7A07_002066"
     CDS             6603..7775
                     /gene="emrA"
                     /locus_tag="N7A07_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU2476732.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7792..9330
                     /gene="emrB"
                     /locus_tag="N7A07_002067"
     CDS             7792..9330
                     /gene="emrB"
                     /locus_tag="N7A07_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU2476733.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9394..9909)
                     /gene="luxS"
                     /locus_tag="N7A07_002068"
     CDS             complement(9394..9909)
                     /gene="luxS"
                     /locus_tag="N7A07_002068"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU2476734.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10059..11615)
                     /gene="gshA"
                     /locus_tag="N7A07_002069"
     CDS             complement(10059..11615)
                     /gene="gshA"
                     /locus_tag="N7A07_002069"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU2476735.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11688..12116)
                     /locus_tag="N7A07_002070"
     CDS             complement(11688..12116)
                     /locus_tag="N7A07_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU2476736.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12113..12679)
                     /gene="yqaB"
                     /locus_tag="N7A07_002071"
     CDS             complement(12113..12679)
                     /gene="yqaB"
                     /locus_tag="N7A07_002071"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU2476737.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12960..13036)
                     /locus_tag="N7A07_002072"
     tRNA            complement(12960..13036)
                     /locus_tag="N7A07_002072"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13000..13002),aa:Arg,seq:acg)
     gene            complement(13139..13215)
                     /locus_tag="N7A07_002073"
     tRNA            complement(13139..13215)
                     /locus_tag="N7A07_002073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13179..13181),aa:Arg,seq:acg)
     gene            complement(13278..13354)
                     /locus_tag="N7A07_002074"
     tRNA            complement(13278..13354)
                     /locus_tag="N7A07_002074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13318..13320),aa:Arg,seq:acg)
     gene            complement(13419..13495)
                     /locus_tag="N7A07_002075"
     tRNA            complement(13419..13495)
                     /locus_tag="N7A07_002075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13459..13461),aa:Arg,seq:acg)
     gene            complement(13499..13591)
                     /locus_tag="N7A07_002076"
     tRNA            complement(13499..13591)
                     /locus_tag="N7A07_002076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13555..13557),aa:Ser,seq:gct)
     gene            complement(13907..14092)
                     /gene="csrA"
                     /locus_tag="N7A07_002077"
     CDS             complement(13907..14092)
                     /gene="csrA"
                     /locus_tag="N7A07_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU2476738.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14327..16957)
                     /gene="alaS"
                     /locus_tag="N7A07_002078"
     CDS             complement(14327..16957)
                     /gene="alaS"
                     /locus_tag="N7A07_002078"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU2476739.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17085..17585)
                     /gene="recX"
                     /locus_tag="N7A07_002079"
     CDS             complement(17085..17585)
                     /gene="recX"
                     /locus_tag="N7A07_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU2476740.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17653..18714)
                     /gene="recA"
                     /locus_tag="N7A07_002080"
     CDS             complement(17653..18714)
                     /gene="recA"
                     /locus_tag="N7A07_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU2476741.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18794..19291)
                     /gene="pncC"
                     /locus_tag="N7A07_002081"
     CDS             complement(18794..19291)
                     /gene="pncC"
                     /locus_tag="N7A07_002081"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU2476742.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19436..20521)
                     /gene="mltB"
                     /locus_tag="N7A07_002082"
     CDS             complement(19436..20521)
                     /gene="mltB"
                     /locus_tag="N7A07_002082"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU2476743.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         4801 a         5586 c         5183 g         5100 t
ORIGIN      
        1 aaattaaaaa tctttataaa atatttggtg agcatccaca gcgagcgttc aaatatatcg
       61 aacaaggact ttcaaaagaa caaattctgg aaaaaaccgg gctatcgctt ggcgtaaaag
      121 acgccagtct ggccattgaa gaaggcgaga tatttgtcat catgggatta tccggctcgg
      181 gtaaatccac aatggtacgc cttctcaatc gcctgattga acccacccgc gggcaagtac
      241 tgattgatgg tgtggatatt gccaaaatat ccgacgccga actccgtgag gtgcgcagaa
      301 aaaagattgc gatggtcttc cagtcctttg ccttaatgcc gcatatgacc gtgctggaca
      361 atactgcgtt tggtatggaa ttggccggaa ttaatgccga agaacgccgg gaaaaagccc
      421 ttgatgcact gcgtcaggtc gggctggaaa attatgccca cagctacccg aatgaactct
      481 ctggcgggat gcgtcaacgt gtgggattag cccgcgcgtt agcgattaat ccggatattt
      541 tattaatgga tgaagccttc tcggcgctcg atccattaat tcgcaccgag atgcaggatg
      601 agctggtaaa attacaggcg aaacatcagc gcaccattgt ctttatttcc cacgatctcg
      661 atgaagccat gcgtattggc gaccgaattg ccattatgca aaatggtgaa gtggtacagg
      721 tcggcacacc ggatgaaatt atcaataatc cggcgaatga ttatgtccgt accttcttcc
      781 gtggcgttga tattagtcag gtattcagtg cgaaagatat tgcccgccgg acaccgaatg
      841 gcttaattcg taaaacccct ggcttcggcc cacgttcggc actgaaatta ttgcaggatg
      901 aagatcgtga atatggctac gttatcgaac gcggtaataa gtttgtcggc gcagtctcca
      961 tcgattcgct taaaaccgcg ttaacgcagc agcaaggtct tgatgcggcg ctgattgatg
     1021 cgccgttagc agtcgatgca caaacgcctc ttagcgagtt gctctctcat gtcggacagg
     1081 ctccctgtgc ggtgcccgtg gtcgacgagg accaacagta tgtcggcatc atttcgaaag
     1141 gaatgctgct gcgcgcttta gatcgtgagg gggtaaataa tggctgatca aaataatccg
     1201 tgggatacca cgccagcggc ggacagtgcc gcgcaatccg cagacgcctg gggtacaccg
     1261 gcgactgcac cgactgacgg cggtggtgct gactggctga ccagtacgcc tgcgccaaac
     1321 gtcgagcatt ttaatattct cgatccgttc cataaaacgc tgatcccgct cgacagttgg
     1381 gtcactgaag ggatcgactg ggtcgttacc catttccgtc ccgtcttcca gggcgtgcgc
     1441 gttccggttg attatatcct caacggtttc cagcaattgc tgctgggtat gcccgcaccg
     1501 gtggcgatta tcgttttcgc tctcatcgcc tggcagattt ccggggtcgg aatgggcgtg
     1561 gcgacgctgg tttcgctgat tgccatcggc gcaatcggtg cctggtcgca ggcaatggtt
     1621 actctggcgc tggtgttaac cgccctgctg ttctgtatcg tcatcggttt gccgttgggg
     1681 atctggctgg cgagaagtcc gcgagcggcg aaaattattc gtccactgct tgatgccatg
     1741 cagaccacgc cagcgtttgt ttatctggtg ccaatcgtca tgctatttgg tatcggtaac
     1801 gtgccgggcg tggtggtgac gatcatcttt gctctgccgc cgattatccg tctgaccatt
     1861 ctggggatta accaggttcc ggcggatctg attgaagcct cgcgctcatt cggtgccagc
     1921 ccgcgccaga tgctgttcaa agttcagtta ccgctggcga tgccgaccat tatggcgggc
     1981 gttaaccaga cgctgatgct ggccctttct atggtggtca tcgcctcgat gattgccgtc
     2041 ggcgggttgg gtcagatggt acttcgtggt atcggtcgtc tggatatggg gcttgccacc
     2101 gttggcggcg tcgggattgt gatcctcgcc attatcctcg atcgtctgac gcaggccgtt
     2161 gggcgcgact cacgcagtcg cggcaaccgt cgctggtaca ccactggccc tgtcggtctg
     2221 ctgacccgcc cattcattaa gtaactctgc acttgcccgg tgacgccggg catatcaccc
     2281 tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg tttgccacgc
     2341 ttatctctac acaaactttt gctgccgacc tgccgggcaa aggcattact gttaatccgg
     2401 ttcagagcac catcaccgaa gaaactttcc agacgctgct ggtcagccgc gcgctggaga
     2461 aattaggtta taccgtcaat aaacccagcg aagtggatta caacgttggc tacacctcgc
     2521 ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat gacaacatgt
     2581 acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcgg
     2641 cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc aacatcgcac
     2701 aactgaaaga tccgaagatc gccaaactgt tcgataccaa cggcgacgga aaagcggatt
     2761 taaccggttg taaccctggc tggggctgcg aaggtgcgat caaccaccag cttgccgcgt
     2821 atgaactgac caataccgtg acgcataatc aggggaacta cgcggcgatg atggccgaca
     2881 ccatcagtcg ttacaaagag ggcaaaccgg tgttttacta cacctggacg ccgtactggg
     2941 tgagtaatga gctgaagcca ggcaaagatg tggtctggtt gcaggtgccg ttctctgcac
     3001 tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac ggcttcccgg
     3061 tcagcaccat gcatatcgtt gccaataaag cctgggccga gaaaaacccg gcagcagcga
     3121 aactgtttgc cattatgcag ttgccagtgg cagatattaa cgcccagaac gccattatgc
     3181 atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg atcaaagccc
     3241 accagcaaca gttcgatggc tgggtgaatg aggcgttggc agcgcagaag taatttttat
     3301 tcgggcggat aaggcgttta cgccgcatcc gccgttctgt acacaatgcc tgatgcgacg
     3361 ctggcgcgtc ttatcatgcc tacaaaccac accgcacatt tcaacaatct atctttcatc
     3421 ccatattcat caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa
     3481 aataatgacc aaacctaatc atgagcttag cccggcgcta atcgtgctga tgtctatcgc
     3541 caccggtctg gcggtcgcca gcaactatta cgcccagcca ttgctcgaca ccatcgcgcg
     3601 taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc agttgggcta
     3661 tgccgccggt ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat
     3721 tgtctcgatg accttactgg cagcgggcgg tatgttaatc actgccagca gtcagtcact
     3781 ggcgatgatg atcctcggta cggcattaac cggtttattc tcagtcgtgg cacaaattct
     3841 ggttccgctg gcagcgacgc tggcttcacc ggataaacgc ggcaaagtgg ttggcaccat
     3901 tatgagcggt ctgctgttgg ggatattgct ggcacgaacc gttgccgggt tactggcaaa
     3961 cctcggcggc tggcgcaccg tcttttgggt tgcctcggtg ttaatggctc tgatggcgct
     4021 ggcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt
     4081 gttgggttcc gttttcagca tgtttatcag caataagatc ctgcgcaccc gcgcgttgct
     4141 gggctgcctg acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc
     4201 cgctccacct tttaactaca gcgatggtgt aattggtctg tttgggcttg cgggagctgc
     4261 cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgac aagggcaaat cgcaccacac
     4321 cacaactttc ggtctgcttc tgctgttact ttcatggctg gcaatctggt ttggtcacac
     4381 ttccgtactg gcgttgatta tcggcattct ggtgctggac ctcaccgtac agggcgtgca
     4441 tatcactaac cagacggtaa tttatcgaat acatcctgat gcgcgtaatc gcctgaccgc
     4501 aggttacatg accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc
     4561 ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa
     4621 cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa
     4681 ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta
     4741 agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag
     4801 ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca aagacagttt
     4861 accgattgtt attagttata tcccggtggc ctttgcgttc ggtctgaatg cgacccgtct
     4921 gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca
     4981 gttcgtcatt accgcgatgc tggcagcagg gagtagtttg tgggttgctg cactgaccgt
     5041 catggcaatg gatgttcgcc atgttttgta tggcccgtca ctgcgtagcc gtattattca
     5101 gcgtctgcaa aaatcgaaaa ccgccctatg ggcgtttggc ctgacggatg aggtttttgc
     5161 cgccgccacc gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg
     5221 cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg
     5281 cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc
     5341 actctttatg agtttcctgc tcgcctcttt tcagcgcaaa caatctcttt gcgttaccgc
     5401 agcgttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc
     5461 aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga
     5521 tgagctatga ggttctgctg cttgggttac tggttggcgc ggcgaattat tgcttccgct
     5581 atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta
     5641 ttttgctcga caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac
     5701 cagaagtgat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg
     5761 gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct
     5821 atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac
     5881 aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa tgaggtcata
     5941 cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc
     6001 acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcat atgcaaagca
     6061 agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta
     6121 tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt
     6181 gtgctcttgg atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg
     6241 gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg
     6301 aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac
     6361 tctggtccgc gctcagcaca acagaaaaag accatctcga gcaaatcacc cgcaaattgc
     6421 tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg
     6481 tcatctcgct caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat
     6541 gctggccttt ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca
     6601 atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac
     6661 gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga
     6721 tttattggtt tttggtactg cgtcacttcg aagaaaccga tgacgcatac gtggcaggga
     6781 atcaaattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca
     6841 ccgattttgt aaaagaaggc gacgtgctgg tcactctcga cccgacagat gctcgccagg
     6901 cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac cagcagatga
     6961 ttaacagcaa gcagttgcag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac
     7021 aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc
     7081 tgcaacacgc ccgcgacgcc gttaccagtg cccaggcgca actggacgtc gcgattcaac
     7141 aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc
     7201 aacaggctgc caccgaagta cgtaacgcct ggctggcgct ggagcgtact cgtattgtca
     7261 gtccgatgac cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa
     7321 cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag
     7381 agacgcagat tgccaatatg cgtatcggtc agccggtcac tatcaccacc gatatttacg
     7441 gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcgt
     7501 tctcactgct tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctgc
     7561 ctgtgcgtat cgaactggac cagaaacagc tggagcaata cccgctgcgt atcggtttat
     7621 ccacgctggt gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac
     7681 gttccactcc ggtagcggta agcaccgcgc gtgaaatcag cctggcacct gtcaataaac
     7741 tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag
     7801 caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actagcgaca
     7861 ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat
     7921 cttggctcat cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc
     7981 atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctc
     8041 tggtcaacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag cagcctgaat
     8101 atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat cccgctttcg
     8161 caaagtctat tgctgaataa ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg
     8221 tcgatgacgg tgattgtcgc gccaatttgc ggcccaatcc tcggcggata tatcagcgat
     8281 aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc ggtggtgttg
     8341 atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg gattgatgcc
     8401 gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct cgaccgcggt
     8461 aaagagctgg actggttttc atcacaggaa attatcatcc ttaccgtggt ggcggtggtg
     8521 gctatctgct tcctgatcgt ctgggagctg accgacgata acccgatagt cgatctgtcg
     8581 ttgtttaagt cgcgcaactt caccatcggc tgtttgtgta tcagcctcgc gtatatgctc
     8641 tacttcggcg cgattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg
     8701 acctgggcag gtttggcctc tgcgccggta gggattattc cagtgatcct gtcgccgatt
     8761 atcggccgct tcgcgcataa actggatatg cgccgactgg taaccttcag ctttattatg
     8821 tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg
     8881 tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc
     8941 accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctccaac
     9001 tttacgcgta cgctggcggg gtctatcggc acgtcgataa ccacaaccat gtggaccaac
     9061 cgcgagtcga tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc
     9121 caggcgatgt acagtcaact ggaagggctt gggatgacac aacagcaggc atcaggctgg
     9181 attgcccagc agatcaccaa tcaggggctg attatttccg ccaatgagat cttctggatg
     9241 tcggccggga tattcctcgt cttgctgggg ctggtgtggt ttgctaaacc gccctttggc
     9301 gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc
     9361 ttttttcaat taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc
     9421 tttcggcagc gccagttctt cgttgctgtt gatgcgcacg tcacgttcca gaatgctacg
     9481 cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt
     9541 cagctccgga atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca
     9601 gacatcagca acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg
     9661 gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat
     9721 aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca cttctttgtt
     9781 cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt
     9841 tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa
     9901 caacggcatt tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca
     9961 ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg
    10021 aaattttggc cactcatgag tggccttttt cttttctgtc aggcgtgttt ttccagccac
    10081 accgcaaacg gttcggtatc tgcggcttcc atttcctgct gacggcgttc agatgcctcg
    10141 cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg cagcagatta
    10201 cggtacgctt cagcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt
    10261 aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag ttcatcacac
    10321 actttctgat acgcttcgcc gccgttaatg ctatccagcg tttgcgcgac gcgtttcaga
    10381 tcgcggaaca gatctttacc cacctgcggt aacgggaact gtgcggtttc gcagccgata
    10441 cccagcgtca gacccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta
    10501 caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac
    10561 aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc
    10621 agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggcgactcg
    10681 ccgctgcggg taacgcgttt tggacgaatc ggcgcgtaca gttcgttttc aatctgcagc
    10741 acgttgctgt tgatttgcag cctcttaccg tctttatcaa taccaatctt cgcgtactct
    10801 tccgatggcg ttttgattgc ctgtttcagg cccgctacgt actcgtaaag atcgttgaag
    10861 gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag
    10921 gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt
    10981 ttaccttgca ggaatgaaga gcatattgcc ggagacgcgc caaacagata aggaatgacc
    11041 caaccgaagc gatagtagtt gcgaataacg cggaaatagc ccgcagagat tttctcttta
    11101 gcatcagcgc ccgaaatatc gccacacttc gcttgccaga atgccatagg caaagagaag
    11161 ttgtaatgca cgccggagat ggtttgcatc agcgcgccgt agcgattttt cagcccttca
    11221 cggtacagcg ttttaaagcg tccggtgtta gaagtgccgt actgcgccag ttcgatgtcc
    11281 tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg
    11341 cgcgccgtat aacgatgcag atcgcgcata aaggtcagca tatgttcaat atcaccatcc
    11401 actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttatgcgtc
    11461 agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc agcattaaca
    11521 cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc
    11581 agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg
    11641 ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac
    11701 atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt accaaggcac
    11761 aaaaaaaaga ttaccggccc ccaggagatg cgcatccatc ccgctaacag acacagtaaa
    11821 tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag
    11881 ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg gacagaaacg cccaaggata
    11941 acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga
    12001 tgactgatcc cagaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt
    12061 gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca
    12121 agcgaacatc cacagcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat
    12181 cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg
    12241 tgtctggcgc gggtttatgg tgtttgacgt gatcggcagc gacgacggcg tcaaaataac
    12301 ggcgtaatcc caggtgagcc agcaatgcct cagcgatggc gctttcactc cccgttccta
    12361 cagccattgg gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga
    12421 cactatccag cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggt
    12481 cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat
    12541 taagcgcaat catcgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc
    12601 acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac
    12661 ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt
    12721 aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagta
    12781 gttatttaga aatgagatat ttttgaagga aattttttgg agaagatggt gcatccggga
    12841 ggatgactca ctggcgttcg cccttcgggc cgttgctaaa gcaacgttat ccttcctggt
    12901 gcctgcggtt aattccgcta cctcttcgat atcttcattg ctgaagattt cggagaatat
    12961 ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca
    13021 gctgagctac ggatgcatcg ggaaaattta ttttactgct gatattgata tcgctactaa
    13081 agccatatca aataagaaaa tccatcttaa ccatcatcac tgatgattac ggagaatatg
    13141 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    13201 ctgagctacg gatgcatcgg gaaatttatt ttactgctga tattgatacc gctactaaag
    13261 ctgtatcaag taagagatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    13321 agccgagtac tctatccagc tgagctacgg atgcatcggg aaacttactt tactgcagat
    13381 tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg gaggattcga
    13441 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg
    13501 gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg
    13561 ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac
    13621 ttgtttatcg ctcatcgtcg ctgcgtggcg cacatattac tttctgagac ttataagtca
    13681 aacaattttt cccacacttt tatcgtttgc acacttcacg ctcaattagt ctgtaaaaac
    13741 gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa
    13801 aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag
    13861 aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact
    13921 gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct
    13981 tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga
    14041 cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct
    14101 ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc
    14161 caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta
    14221 aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta
    14281 atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg
    14341 cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac
    14401 cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac
    14461 caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag
    14521 aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta
    14581 aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca
    14641 gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag
    14701 cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc
    14761 gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc
    14821 gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac
    14881 gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc
    14941 ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta
    15001 cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga
    15061 gatccatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt
    15121 cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca
    15181 ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg
    15241 cagcgtgcat caggtgcgtt gcggagtgat tcaaacgaat acgggcgcga cgagcctcat
    15301 caacatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt
    15361 gaccaatcgc ctggccgtat ttctgcgtat cttccaccac gaaggagaag ttagcgcctt
    15421 tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggc gtttgatcca
    15481 gcacgaccac agcttcctgg cctgcattga tggcatcaac cgctttacca tcaacaaaca
    15541 gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactgt
    15601 caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcccgacgac
    15661 gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac
    15721 aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagcagttt
    15781 caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc
    15841 gctccagagt acgagcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct
    15901 gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt
    15961 tgtagaagaa ggtttctttc gcgccgagca tattaccgtg acgcactgcg cgacgaatga
    16021 tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga
    16081 acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggttg
    16141 cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag
    16201 agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg
    16261 gcttcggcag cggttccatc gtgccatcgg cctggcggtt gaactgcatg aagacgatgt
    16321 tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa
    16381 tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca
    16441 tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac
    16501 gttcgcgcgg gatccctact tctttttccc agatttcgta ggcttcgtcg tcgctttcat
    16561 agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt
    16621 cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca
    16681 tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac
    16741 cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc
    16801 caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt
    16861 tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga
    16921 gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac
    16981 gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga
    17041 ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt
    17101 cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc
    17161 ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg
    17221 cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg
    17281 gaaatacctt tctgattcag ttcctggcga atacgcgcag gtccataacc tttgcggcta
    17341 cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac
    17401 caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg
    17461 cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc
    17521 agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga cgttgattct
    17581 gtcatggcat atccttacaa cttaaaaagc aaaagggccg cagatgcgac ccttgtgtat
    17641 caaacaagac gattaaaaat cttcgttagt ttctgctacg ccttcgctat catctacaga
    17701 gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct tctcgatctc
    17761 tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac cctgaccgat
    17821 cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct cttttacgcc
    17881 caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct ggaattcagc
    17941 ctgtttaaac ggcgcagcga ttttgttctt caccactttc acgcgggttt cgctacccac
    18001 cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa cagaggcgta
    18061 gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca caccaatttt
    18121 catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt tacccgccag
    18181 cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc
    18241 gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga taacgtctac
    18301 tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg tgtccggctg
    18361 ggagcacagc aggttgtcga tatcgacgcc cagtttacgt gcgtagattg ggtccagcgc
    18421 gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg caatcacctg
    18481 caacgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga tacggcccat
    18541 cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag agatggtttc
    18601 cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa attgtttctc
    18661 aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac
    18721 tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaagcaa ttatactgta
    18781 tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc aatgcatacg
    18841 cagtagcctg acgacgcacc gcatcacggt cgccgctgaa gcattcccgc agggtaatgc
    18901 cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc tcttcactgc
    18961 cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga gccgctttca
    19021 gtgcacctat cgccatttcc accacgacgg gttcactcac cgcgccatgc tgcgccagcg
    19081 tctcttcgcg tacgccgatc atttgcgctt tggcttcgtt actgtaggtg acaaatccgc
    19141 gttcaaacca ggcggaactg ccggcaatat cggtaatcac tttcgctacc caaccgccgg
    19201 tacaagactc agcagttgtt acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt
    19261 cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc
    19321 cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt acgaggagga
    19381 ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgca aatagttact
    19441 gcacgcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg taatgggtac
    19501 tgtggttata acgggtaatg gtgtagaagt tcggcagacc gtaccagtac tggtaacctg
    19561 ttccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc tgctgtggcg
    19621 ttaaacctgc cgcggcaagc tgcgaaatgc tgtacctggt tttgaagcca tttggcaagc
    19681 ctggagcctg accgtttgcc attaccgcga cctgatcgcc tttcacccag ccgtgcgctt
    19741 tgaaataatt cgccacgcta ccgatcgcat caaccggatc ccacaggttg atatgcccgt
    19801 cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt ccgtagccca
    19861 tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg cgcgccatca
    19921 gcaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca
    19981 gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag cgggtttcaa
    20041 cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac gcgcgattca
    20101 acgcatcttc atactgattc cagaaaacca caccgttctg cacattgtcc ggcgtaataa
    20161 attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc accgatgtgg
    20221 ttggtgcctg gttatccatc agccgcagta ccgaatccag acgtttcgcc tgggagagaa
    20281 tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattctgtcg atgaactgct
    20341 gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa
    20401 gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt tttggcttgc
    20461 tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga cgcttgaaca
    20521 tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc acgccccgca
    20581 caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca ttctcctcgc
    20641 tatcctttta ctcaaacttt caaattaaaa
//
LOCUS       DBFIFS010000061        20339 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.061, whole genome shotgun sequence.
ACCESSION   DBFIFS010000061 DBFIFS010000000
VERSION     DBFIFS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.061"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(387..1133)
                     /gene="cutC"
                     /locus_tag="N7A07_002083"
     CDS             complement(387..1133)
                     /gene="cutC"
                     /locus_tag="N7A07_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU2476744.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1147..1713)
                     /gene="yecM"
                     /locus_tag="N7A07_002084"
     CDS             complement(1147..1713)
                     /gene="yecM"
                     /locus_tag="N7A07_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2476745.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1929..3662
                     /gene="argS"
                     /locus_tag="N7A07_002085"
     CDS             1929..3662
                     /gene="argS"
                     /locus_tag="N7A07_002085"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU2476746.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3704..3748)
                     /locus_tag="N7A07_002086"
     CDS             complement(3704..3748)
                     /locus_tag="N7A07_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476747.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3839..4327
                     /gene="yecT"
                     /locus_tag="N7A07_002087"
     CDS             3839..4327
                     /gene="yecT"
                     /locus_tag="N7A07_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU2476748.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4447..4839)
                     /gene="flhe"
                     /locus_tag="N7A07_002088"
     CDS             complement(4447..4839)
                     /gene="flhe"
                     /locus_tag="N7A07_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU2476749.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4839..6917)
                     /gene="flhA"
                     /locus_tag="N7A07_002089"
     CDS             complement(4839..6917)
                     /gene="flhA"
                     /locus_tag="N7A07_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU2476750.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6910..8058)
                     /gene="flhB"
                     /locus_tag="N7A07_002090"
     CDS             complement(6910..8058)
                     /gene="flhB"
                     /locus_tag="N7A07_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU2476751.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8260..8904)
                     /gene="cheZ"
                     /locus_tag="N7A07_002091"
     CDS             complement(8260..8904)
                     /gene="cheZ"
                     /locus_tag="N7A07_002091"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU2476752.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8915..9304)
                     /gene="cheY"
                     /locus_tag="N7A07_002092"
     CDS             complement(8915..9304)
                     /gene="cheY"
                     /locus_tag="N7A07_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU2476753.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9319..10368)
                     /gene="cheB"
                     /locus_tag="N7A07_002093"
     CDS             complement(9319..10368)
                     /gene="cheB"
                     /locus_tag="N7A07_002093"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU2476754.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10371..11231)
                     /gene="cheR"
                     /locus_tag="N7A07_002094"
     CDS             complement(10371..11231)
                     /gene="cheR"
                     /locus_tag="N7A07_002094"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU2476755.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11250..12851)
                     /gene="tap"
                     /locus_tag="N7A07_002095"
     CDS             complement(11250..12851)
                     /gene="tap"
                     /locus_tag="N7A07_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU2476756.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12897..14558)
                     /gene="tar"
                     /locus_tag="N7A07_002096"
     CDS             complement(12897..14558)
                     /gene="tar"
                     /locus_tag="N7A07_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU2476757.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14701..15204)
                     /gene="cheW"
                     /locus_tag="N7A07_002097"
     CDS             complement(14701..15204)
                     /gene="cheW"
                     /locus_tag="N7A07_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU2476758.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15225..17189)
                     /gene="cheA"
                     /locus_tag="N7A07_002098"
     CDS             complement(15225..17189)
                     /gene="cheA"
                     /locus_tag="N7A07_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU2476759.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17194..18120)
                     /gene="motB"
                     /locus_tag="N7A07_002099"
     CDS             complement(17194..18120)
                     /gene="motB"
                     /locus_tag="N7A07_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU2476760.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(18117..19004)
                     /gene="motA"
                     /locus_tag="N7A07_002100"
     CDS             complement(18117..19004)
                     /gene="motA"
                     /locus_tag="N7A07_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU2476761.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(19131..19709)
                     /gene="flhC"
                     /locus_tag="N7A07_002101"
     CDS             complement(19131..19709)
                     /gene="flhC"
                     /locus_tag="N7A07_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU2476762.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19712..20062)
                     /gene="flhD"
                     /locus_tag="N7A07_002102"
     CDS             complement(19712..20062)
                     /gene="flhD"
                     /locus_tag="N7A07_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU2476763.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4899 a         5577 c         4933 g         4930 t
ORIGIN      
        1 gattcctcta attaaactcc agaacaccga aatcattgta taaataaata cacaacgaat
       61 tgactacttg cttaattcgg cacaacactg cctgaaataa tcgctaaaga atatgattta
      121 ttacaatgta atcattaatt gctaaggaat aatccagttg ttatttagaa tatatatttg
      181 tcagttttag caatttaaat cacataatta atgtgaatat ataaaatatt tttagcacaa
      241 cgctatgtgc actgtcaccc gctcaacaga tcgcggtaat cgttcatttt ttatactatt
      301 cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat
      361 tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc
      421 atttcagcaa ccgccgcccc gtctacgaca taacgcgaat actcgtccgc gtgtgcatct
      481 gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg
      541 gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact
      601 cctgctccgg ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat
      661 aaaccttgca gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc
      721 gcaagattat tgagtgtatt tagagggtta gcgcacatat caaaggcgcg atgaaaagtc
      781 actgccagcg gaccggcagc agccattatt ttttccattc gtgacatatc gacattaccg
      841 tcaacatcga gcacgcccgt cacgagtcca ggaaaaccta attcgcggac cgtgcgcaca
      901 tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga
      961 attatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac acccaacgac
     1021 ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc
     1081 tgcgccgtta gcgcgcattc catgctgtaa cagcaaattt ccagtaatgc catttttgct
     1141 ccttaattac gccgactgct cgctggcaac aatctcttca atggaccacg gatgaaattt
     1201 aatggtcgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgtt catgctcacc
     1261 ttttggcgaa ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa
     1321 tgccagcgca cgggcattca gcgtttctgg atcacccggc aaaacgattt caatatgttc
     1381 ccaaccttcg tgcgggtaac gtttttccct aggccacggt agctccacaa tagaaaactg
     1441 ccagtgtgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat
     1501 catattttct gacaaaagct cgccacactg ttcaaacccg cgacgccagc gctcagcagt
     1561 ggcgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga tattcaaccc
     1621 cagacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc
     1681 ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc
     1741 gttaatttac cctgttgccc tgcgccaacc aaccgctgat ttcacgccgc ttctgatgca
     1801 atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat
     1861 actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag
     1921 gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg
     1981 caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg
     2041 actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat
     2101 tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg
     2161 ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc
     2221 aggcgctggc gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg
     2281 actactctgc gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta
     2341 ttggtgacgc agcagtgcgt actctggagt tcctcggtca caaagtgatt cgcgcaaacc
     2401 acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc
     2461 aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa
     2521 agcattacga cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc
     2581 aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc
     2641 agaaccagat cacttacgat cgtctcaacg tgacgctgac ccatgatgac gtgatgggcg
     2701 aaagcctcta caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg
     2761 cagtagaaag cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg
     2821 aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata
     2881 tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg
     2941 actcccgtca gcatcaacac ctgatgcagg catgggctat cgtccgtaaa gcaggctatg
     3001 taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca
     3061 aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc cgatctgctg gatgaagccc
     3121 tggaacgtgc acgccgtctg gtggcagaaa agaacccgga tatgccagcc gacgagctgg
     3181 aaaaactggc taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc
     3241 gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc
     3301 catacatgca gtatgcatac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattgacg
     3361 aagagcaact ggctgcagct ccggttatca tccgtgaaga tcgtgaagcg caactggcag
     3421 ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg
     3481 taatgtgtgc ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc
     3541 cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga
     3601 cagcgaagac gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt
     3661 aatcgatttt tcgtgagagt gaagcctgat caggtttagc cgatcaggct tttttattac
     3721 catctaaatg tattctgaaa ataggcatgc cattgttttc tcgctgttga gtaagaggcc
     3781 agaagcgtaa tatccagctc cagggaaacg gtaacggttc aatttaagga ataccgcagt
     3841 gtttaaattt cttgtattaa cattaggcat tatctcttgc caggtttacg cagaagatac
     3901 gcttatagta aatgatcatg acatttcagc catcaaagat tgttggcaaa aaaattcagg
     3961 tgatgatact gacattaacg tgatcaaatc atgtctgcga caagaataca atctcgtcga
     4021 tgcgcaatta aataaagctt atggtgaagc ttatcgttat atagaacaag tgccacgcac
     4081 aggtgcaaaa aaacctgata ctgaacaact taacttgctt aaaaaatcac aacgagcctg
     4141 gctggatttt agggacaaag aatgtgaatt aatcctttca aatgaggacg ttcaggattt
     4201 aagtaaccct tattctgaat cagaatggct atcatgtatg atcatacaga ccaatacgcg
     4261 aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc ctttgacaag
     4321 aggataattc acatattttt ggcatgtttt gttgcaagct attcctgata aatgattgca
     4381 acaaaacatc cagccttttt cactgagtta ttaaacatac tcgcgagcgc gtaatttttt
     4441 tgtccttcag cgataattca caatcacttc attacgttgt actttcagcg acggaattaa
     4501 ccgcccaccg ccaggcactt cccagataaa gcgcaacggt tctgctgccg ctataccgga
     4561 aaaggccacg gtggttccgc tctgcccatc taattcgaca caacgagatt gcgaacacaa
     4621 gcgaacccgc agtcctgcag gtgtcgggcc gataagctga taccgccacg ccaccagcgt
     4681 catcaatcct gaagcaggtt gtcgcgtaga aagaggtgca gacgacatcg actcaccgcg
     4741 atgatttagc gtaattccga cactacttgc ctgccacatc ccctccccgg cggcttgcac
     4801 cagcagcgga aacaataata ttgctaataa ggttctcatc atttaccgcc aattgtcgcc
     4861 gtcatgcgga tatgtcggtt atcagacagt tccagattcg acagcaccac taactgcggc
     4921 aagctgcggc gcagaaagcg agacaataat ggtcgcagcg cgtggttcac caataaaact
     4981 ggcggcgcac ccaacatctc ctgacgggat agcgcttcct gagtttgcgc cagtaaacga
     5041 tccgccagcc ctggctccag tccgcccccg ccctgcaacg cctgtagcaa caaacgttcc
     5101 agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca ctgctgggta
     5161 atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt catgtggatc gctttggatg
     5221 ggcgcatgtt ccgccagcgt ttcgagaata gtgcgcatat cgcgaatcgg tactttttca
     5281 tcgaggagat tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc agggacgaga
     5341 tcttccgtca gctttggcat ctcctgggcg acgcgatcca gcagctgttg cgcctcctga
     5401 cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt tgctaccacc
     5461 gtgctggcct caaccactgt gtacccctga atctgcgcct gttcttttag cgcactttca
     5521 atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg taacgtcccg
     5581 gcagcggttc cagggttaat agccagccag cgccccggat aagcatcacc actgcctatc
     5641 tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg
     5701 tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt tcttgcggat actgcgtata
     5761 cggcccaaca actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg ataacccact
     5821 tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac aacggcatta
     5881 ttctctgcca tttttaccgg ttttggttcg gcaggcgctt tttgttcgcg tccacggatc
     5941 caccaggcca gccccagcaa tccggcagtg aacagcaaaa ataccaggtt cggcattcca
     6001 ggcaccaggc cgagtaaacc gagcacggca gcgcttaaca acataacgct tgggttactg
     6061 aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg cgtaacaatg
     6121 acccccgcgg cggtagaaat caccagagcc gggatttgtg ccaccagacc gtcaccaatg
     6181 gtcaagagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg cagcacgccg
     6241 accagcaacc cgccgacaac gttaatgacc atgatgagga tcccggcgat ggcatcgccg
     6301 cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg agtgacttcg
     6361 gagcggcgtt ttttcgcctc atcttcacca atcaatccgg cgttaaggtc ggcgtcaatc
     6421 gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc tgcgatacgc
     6481 ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacaaa caccacgata
     6541 ccgatagcga aattgccacc aacgaggaag tgaccgaacg cttcaaccac cttccctgcc
     6601 gccgctgcgc cggtatgccc ttccattaaa atgatacggg ttgaagccac gttaagtgcc
     6661 agacgcaaca gcgtggtaaa caacaaaatg gtcggaaacg cagcaaactc aagcgtgcgc
     6721 tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa ggtaaacaac
     6781 aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa caggatcaaa
     6841 atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgtgggcag gcgcagcatc
     6901 gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt ccggcaccgg
     6961 aagatgagta ggttgtacag ggcgctgtcc accagccaga cgccagcgtt tcagttgcca
     7021 gacccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt gttgaccaat
     7081 ctccacatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt
     7141 ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct gcacctttag cgaccacttt
     7201 cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg tcgggttatt
     7261 gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcgg cagctcgctg
     7321 catctgacgg atccgccctt taacatgggg gtcgccttcg ctttgtttga actcatcacg
     7381 aatatcctgc cgcgacattc gcagcttttt caggtggctg aagatttgga aaaagacgtc
     7441 aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc ctaccaaatc
     7501 catcgcatta cccatggcgg taatcggaga ctcggccatt aagcgcatca tatgcggcca
     7561 gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg atggttttca aaattgcttt
     7621 aagcaactcc gcgccagtct gtgccgagaa catccgtttt atgcccggta gcgggttgag
     7681 tttggaaaac ttcggctgca aggattttcc gctaaatacc agccccccca gcataaccgg
     7741 agaaatgagc gccaccagca ccacgccgct aatcagcggt agcagcgcca gcatggcttc
     7801 tctgatcagc agaataattt gcccgaggat cagattcggg tctttgataa tactgtgatc
     7861 aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca caccgccaaa
     7921 ccagataaca ctaacgccca ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg
     7981 gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggctt ctgttttgtc
     8041 gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcgtaa atgatgccag
     8101 agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag gcgggattta gggcttttgc
     8161 tgccacacat caagcatagt gtgcgtttgt cggatgcggc gtcatcgcct tatcagaccg
     8221 cctgatatga cgtggtcacg ccacatcagg caatacaaat caaaatccaa gactatccaa
     8281 caaatcgtcc acctgatcct gactggctac cacaccggct ttgctggtat cgacctgagg
     8341 tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg ggatgttttc
     8401 caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca tccgcttaat
     8461 gacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca gttgcgcgtt
     8521 agtaaagctg gtatgcgcgg gtacatctgc cagaaatttc cgcgtatctg ttaccagttc
     8581 acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt gggttaacgc
     8641 ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac tgttcagcgc
     8701 ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc gcgcatcggg
     8761 gatggcttcc gccgcttcgg caatggcctg atccagtccc agttcccgca aactgtcgcg
     8821 cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc
     8881 aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc tcaaagattt
     8941 tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag ccactggccc
     9001 ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc attaacactg
     9061 gcaatgccga catcgcgcca tccgcacgaa tcgttttcag caattccagg ccatccatgt
     9121 tgggcatgtt ccagtcggag ataacaaatc cataaccgcc tgcctgcaac ttattgagag
     9181 cgtcgacgcc atcttccgct tcctcaacat tattgaatcc cagctctttc agcaggttac
     9241 gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt tctttatccg
     9301 ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattt ttgccaacat
     9361 ttgctggctt acctggttaa gatcgaccac ttcgcagaca ccacccatat tgatggcctc
     9421 gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc atgccccagc
     9481 ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc
     9541 aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta ccgaaggccg
     9601 atgacggtta accgccgggc catcgtgaat tttgatttgg taatttgcgc cactacgcgc
     9661 cagctccata tgccgatcgc ccggcgcaat ataggcatgc cccggcaaaa cacgttcccc
     9721 gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg
     9781 ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc gggctggaaa gcggcaacgg
     9841 ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt
     9901 ttcagaactc aacaacggcc ctgccttcag cgttgtcggt gccgacaatg gcttatgtgc
     9961 tgcaaggctc gcctttgctg ctgtacgcac cttttcagca atcatttcgc tgtacgccag
    10021 cattccttcg cgaataccca gttgcggttt ggtgacaaaa tctattgccc ccagctccag
    10081 cgcgcgcagc gtgacttctg atcctttgcc ggtcagggaa gaaaccatca caacgggcat
    10141 tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac
    10201 atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcga ccagcggatc
    10261 aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg
    10321 gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta
    10381 cttagcgcat acaccgtttg accacgcagc gtgaagcggc gctcgaggtg gctaaagttt
    10441 tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa
    10501 atctcctgct gggtagtttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca
    10561 aacggccccg gcacggtgta ctgtttcgcc agtagattca gcgaggcaaa atcaacatag
    10621 ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa
    10681 taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata gataccgctt
    10741 ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca gcgtccgggc
    10801 gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc gccggtcgaa
    10861 gccgccgcgc tccatacgcg atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga
    10921 gggaaatgat gtgcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac
    10981 gcctgccact caccgctgtg ctgattagat tccagcaagt tcagataatg accgaaatcc
    11041 gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg
    11101 tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaatgcgcg
    11161 tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc acagggcaga
    11221 gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat ttgtaactgc
    11281 gccgactcat gtcgtgctac ttcatgttct tcaagggtaa ataccgccac acgcgacgaa
    11341 agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc taccagcgag
    11401 gcgttctgct gtgtcacctg atccatctgg ctgacagcct gcgcaacctg ctcaatcccc
    11461 cgccgttgtt cttccgaggc cgaagcaatt tctcccataa tgtcgttcac gcgggtcacc
    11521 gaactgacaa tatcggtcat ggtcgcggcg gcgttattca ccagtttcga accctgctgg
    11581 acacgattga ctgactcttc gatcagccct ttgatctctt ttgccgcctg ggcgcttcgg
    11641 ctggcaagat tgcgcacttc tcctgccact accgcaaaac cacgcccctg ctctccggcg
    11701 cgagccgctt ctactgccgc attcagggcc agaatattgg tctggaaagc aattccgtcg
    11761 ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg catggtgtga
    11821 gtcatggtac tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt ttttgcaagt
    11881 tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa ttgctccata
    11941 ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga gagatcgtta
    12001 ttgcctgcga caatctccgc gataccaatg tgcatctcct ggcttccctt acgcacatca
    12061 cttaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc tggcaaaaat ggcggtgatc
    12121 tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat gctgtcaaaa
    12181 tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca cagcgcactg
    12241 ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagcgccga aatctgatag
    12301 ttacgctggc tttgcgcact ggcggcttcc agcacatgga tgatctccag ctgccaggcc
    12361 tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa
    12421 agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa
    12481 cttttctccg tctctttttg caatgccctg acgtgctcgt tgcccgcagt catcgccata
    12541 aaacttttaa acagcgtggt ggattgcgtc agactggcac gcgccgtcgt catcaacgtt
    12601 ttaatgtcat ccgccggata gctaagcgct gtcagagtgc ctgctttgtt cagcgtggta
    12661 ctggcctgta acattaccgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc
    12721 tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg
    12781 ccaatctgca agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata
    12841 cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa
    12901 aatgtttccc agtttggatc ttgttcagca attcgcagtc gtggctgtgc cggtggttgc
    12961 tcactggcag gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacga
    13021 aacgcggaaa ccgcttgcgt taaacgacta gcctgttctt ccagcgcagc tgcagcggca
    13081 gctgattcct gcaccagcga tgcgttctgt tgcgtgacgt gatccatttc cgaaaccgcc
    13141 aatgcgactt gatcaatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata
    13201 atgtcagtca cgcgagtgac agcatcgacg atattgttca ttgtttcccc ggcgctttcg
    13261 accagcaccg aaccggtatc aacgcgcgag acggagtctt caatgagagc tttgatctct
    13321 tttgccgcct gggcgctacg actggcaaga ttacgcactt cgcccgccac cacggcaaaa
    13381 ccacggccct gttcacccgc tcgcgcggct tcaacagcgg cattcagcgc gaggatatta
    13441 gtctggaagg caataccgtc gattacgctg ataatgtcgg cgattttctt cgaactatcg
    13501 gcgatctcat gcatcgtttt cactacgcca tccaccactt tgccgccgtg ctgggcggtg
    13561 tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact
    13621 gtcgcggtga gctgctccat gctggcggca gtttcttcca gcgcggatgc ctgctgttcc
    13681 gtacgggagg aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca
    13741 tctgaacctt cgcggacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg
    13801 ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta
    13861 ccaccggcga tttcgcgaat gtgagcaata atttttgcca gcggtgtaag caacatacgg
    13921 cgaatgccat accacgccac cagcagaatc aataccacca ccagcgcgat aaccgccagt
    13981 tgccactggg caaatcggta atcatctgcg ttgtcagtga cgatatcgcg atacagtttt
    14041 tcactgctga gggcgtactg agcaaacgct tcgcccattg cattttgcat tccctgggtt
    14101 ggctgagcga aataagctcc agtattgcca taatcaagat aatcaatcag ttcagttaac
    14161 gctgtgtaat agtttttata tttttcatca atattacgac tggtagcgac catttcaggt
    14221 aacggtgcca tgcttttgaa ttttttataa tgagtcgcgg cctgggccaa tgttttcctg
    14281 gcgctatcga gcaattcaac tttggcatta ctttgttgat tggaggaatc catcatcatt
    14341 cgcaccgctg aacgactcag gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc
    14401 agctcgccct gctgttctcg taattgattg gaaaccacaa agctcttctg gctatggtga
    14461 agggaagaaa aaaacagact gccggaaata agctgtaaca gtgcgaatac ccccagcacc
    14521 attaccaaca gcgtgactac gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag
    14581 ttgatctcgt tatcggcaag gaagggggaa actttattgc tgatgccacc cgccgcgaaa
    14641 ttgaaataaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcaggagaa
    14701 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
    14761 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
    14821 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
    14881 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
    14941 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
    15001 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
    15061 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
    15121 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
    15181 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
    15241 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
    15301 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
    15361 aggtttttaa ctacaacctg atgttgacca attaattgat ccaccagcaa ggcgtagcgg
    15421 cgaccgccac tttgtaagat caccacaatt ccctgggtag cttcggtttt cgcgcccgcg
    15481 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
    15541 cgctcgccgc cggccagtgg atgcagatcg gcttcacggg gttgcagtga ttccataaca
    15601 gcattcagcg gcagaatgaa tacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
    15661 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
    15721 tcaacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
    15781 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcatcgcg
    15841 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
    15901 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
    15961 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
    16021 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
    16081 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
    16141 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
    16201 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
    16261 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
    16321 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
    16381 ttttctaccg ctacacggat gctggtggat tcactgctac gcgtcgtttt ttcccgctcc
    16441 acgcgaccgg ttggcgcttg ttcggctgcc agtttaagca ctggtggggt ggatattttt
    16501 ggcgagactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
    16561 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
    16621 ttcaccacgt cagttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
    16681 ctggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ctgctcatct
    16741 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
    16801 gcttctaatg ccagttgacg caaggcctgg cagatataat cgaagctggc ggcatccggc
    16861 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
    16921 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
    16981 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
    17041 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
    17101 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
    17161 tgataaaaat cgcttatatc catgctcacg ctgtcacctc ggttcggctg atggcattgt
    17221 gggaacactg acctgtggtg caacctcagg tttttccagg gcgcttactg gctcattctg
    17281 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccagcaggct
    17341 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
    17401 gccgaccaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
    17461 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cactggcgta
    17521 ggggaaatca tcggtatgac ccgaaaggct aatacggttg ggaataccat tcagaacggg
    17581 cgcgatggca cgcagaatgt cgcgcatata gggttcgacg tcggcactgc cagttctaaa
    17641 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
    17701 tttgagatgt ggacgtaacg cccgcagttt cggatcggac tctatcaatt ggtcgagatc
    17761 gccacgcaat ttccgcaatc gactttgctc catgcgtttt ttcaactctt cgatgttcgg
    17821 ctgcttattc acttccccct ggctttgggt gtaatcatcg ccaccgccgg gaattgggct
    17881 ttcactatta gaaatgcgat cgccgcccgt aaccgcagtc gccagtggag tccggaagta
    17941 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    18001 aaaggccatc atcgcggtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    18061 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    18121 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    18181 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    18241 ggcggtgcgt aaccgttcag attagaaagc agtgtgactt tgacgcactg catcattttg
    18301 ctggtttcgg cgcttttctg acgtaaaaca ctcgctaatg gggaaataaa tccgtaagcc
    18361 aataaaatgc cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    18421 gcaggacgat cggctgaacc taacgcgtga acgaccccca ttacagccgc gacaatacca
    18481 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    18541 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    18601 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgatgatg
    18661 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    18721 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    18781 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    18841 ttaatcgctt ttccattatt gccgacaata aacgacccaa tccctgcacc ggcaataatc
    18901 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    18961 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    19021 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gcaaaaaaaa
    19081 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    19141 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    19201 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    19261 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    19321 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag cggtccttct
    19381 tctgcttgcg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    19441 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    19501 ttttgttccc aagtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    19561 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    19621 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    19681 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    19741 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    19801 ggagatcgtc aacgcgggaa tcttgcgtca acagagtaat cgtctggtgg ctgtcaaaac
    19861 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga agagtcagtg
    19921 ccgctaatgt tgtcgccatt tcttcattta tgccgagacg aaacatagcg gacgctttgt
    19981 cctgaacaat cagacgctgt gcaagtagta aatatgacaa gttgatgtca taaatgtgtt
    20041 tcagcaactc ggaggtatgc attattccca cccagaataa ccaactttat ttttatgcgg
    20101 tttcaccgca ccccgtgatg tcgccgggaa gccccggtaa aaaataatta gcattagaat
    20161 agttgcgata agctgcaata agcagagcca cctttttggt ttaataagtc cttacaaata
    20221 gaagggggtc tttacactta tctaagattt ttcctaaatc gacgcaactg tactcgtcac
    20281 tacacgcaca tacaacggag gggggctgcg attttcaata atgtgtgatg cagatcaca
//
LOCUS       DBFIFS010000062        20240 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.062, whole genome shotgun sequence.
ACCESSION   DBFIFS010000062 DBFIFS010000000
VERSION     DBFIFS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.062"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..472
                     /locus_tag="N7A07_002103"
     CDS             <1..472
                     /locus_tag="N7A07_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2476764.1"
                     /translation="ATGERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDT
                     GAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPGNIIETNSHGWFPD
                     TDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            474..743
                     /locus_tag="N7A07_002104"
     CDS             474..743
                     /locus_tag="N7A07_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2476765.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            882..1070
                     /locus_tag="N7A07_002105"
     CDS             882..1070
                     /locus_tag="N7A07_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476766.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            1365..2990
                     /locus_tag="N7A07_002106"
     CDS             1365..2990
                     /locus_tag="N7A07_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476767.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNANIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPTKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPMSIVAYSWRGD
                     YIGCYTKPDGKQDVFVFSPANMDIRYLSTPFDCAWIDLAKDMMRVVTGDKMSVLAGDS
                     LPSMIRWHSKIFSLPERTSFSCIRVKSPAPERVGITVMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2987..4255
                     /locus_tag="N7A07_002107"
     CDS             2987..4255
                     /locus_tag="N7A07_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2476768.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            4270..4548
                     /locus_tag="N7A07_002108"
     CDS             4270..4548
                     /locus_tag="N7A07_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476769.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            4554..5171
                     /locus_tag="N7A07_002109"
     CDS             4554..5171
                     /locus_tag="N7A07_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476770.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            5262..5996
                     /locus_tag="N7A07_002110"
     CDS             5262..5996
                     /locus_tag="N7A07_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2476771.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGITAGIGLKF"
     gene            6227..6367
                     /locus_tag="N7A07_002111"
     CDS             6227..6367
                     /locus_tag="N7A07_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2476772.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            6424..6825
                     /locus_tag="N7A07_002112"
     CDS             6424..6825
                     /locus_tag="N7A07_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476773.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            6920..7576
                     /locus_tag="N7A07_002113"
     CDS             6920..7576
                     /locus_tag="N7A07_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476774.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGGSGGAKTPGTGANAIQYQAQNWRL
                     "
     gene            7579..8025
                     /locus_tag="N7A07_002114"
     CDS             7579..8025
                     /locus_tag="N7A07_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476775.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            8035..8328
                     /locus_tag="N7A07_002115"
     CDS             8035..8328
                     /locus_tag="N7A07_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGU2476776.1"
                     /translation="MGYGLLDIANQSRREALQGISEADRRREEIEASNKMMAAQQKAQ
                     KKQNIATGIGTGAAVGASVAGSSAAMGATLGAWAGPVGIAAGAVIGGIAGALF"
     gene            8339..9604
                     /locus_tag="N7A07_002116"
     CDS             8339..9604
                     /locus_tag="N7A07_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476777.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            9673..18048
                     /locus_tag="N7A07_002117"
     CDS             9673..18048
                     /locus_tag="N7A07_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU2476778.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEEAAMRDAETVQQDDAA
                     PQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRPELLEEYRRLSLKEGRTETEEQQLQAIRDVLRPQQEARPEAQP
                     ENTDDGDGSIYPTVRFRDPDEVRIEINGSGASRPAERIEKVRPDNRYFTDEKSAMGSD
                     VFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPMEQP
                     AFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRLPA
                     PGDIHPGQGYPLPGEVARTPDENQAGRGGRFTITGEVKGQSFQKGRAPAPENAAGRQG
                     ETLEGETVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQ
                     QAEVRRQARENRDILQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAAK
                     HRYAQLAETLGKTLEDRDYVTQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPA
                     KTKLNAVKWVESVKSIRTSVAGMLEGKVTAGRLAEWMEGRMPARYADTWQLLRTLPPS
                     QIDKASAYRVVSGVYQAAGGKRYDPPRKLYSLRNKDNKGSNLFFSESRDELLTKAKAW
                     FAEQEEKSQAKGDEKTALSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDA
                     RKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPKQFSDAFGFRGV
                     QFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHY
                     EPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDFMTEARRVKRVF
                     KDGRFVDAEYPVRQEVYDAFKGVIEAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARA
                     FERYVQDKARKAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLK
                     TRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDL
                     KVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEII
                     HHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLAHM
                     AEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPR
                     EDGAGASSQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRH
                     LGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLL
                     DAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSR
                     DNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQD
                     KFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADY
                     KIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQY
                     DRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGK
                     GFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDY
                     WQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYIKL
                     HDPRLMRAMKNMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNL
                     KAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVE
                     DGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALR
                     LSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRT
                     LGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVP
                     SHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNV
                     VGGVLGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGT
                     PKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRSGAVDVTPESLKYWIDYISGGTGRF
                     ISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSL
                     TGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVE
                     RDMKIAVDRFNREYNKKVGVE"
     gene            complement(18270..19202)
                     /locus_tag="N7A07_002118"
     CDS             complement(18270..19202)
                     /locus_tag="N7A07_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU2476779.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            19496..>20240
                     /gene="mlaA"
                     /locus_tag="N7A07_002119"
     CDS             19496..>20240
                     /gene="mlaA"
                     /locus_tag="N7A07_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU2476780.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDI"
BASE COUNT         5184 a         4528 c         5903 g         4625 t
ORIGIN      
        1 ggcaaccggc gaacggggac cggcaggtga tgctggtccg gcaggcccgc aggggccgaa
       61 aggtgacagg ggagagcggg gagagaccgg tctgacggga aatgcaggtc cacagggtcc
      121 aaagggagat accggtgcgg caggcccggc aggcccacag ggaccgaaag gagaaacagg
      181 tgcggctggc ccggtggggg caaccggacc tcagggaccg aagggcgacc cgggggagac
      241 gcaaatacgg ttccgtctgg ggccgggaaa cattattgag acaaacagcc atggctggtt
      301 cccggataca gatggcgcac tcatcaccgg actgaccttt cttgacccca aagatgccac
      361 acgggttcag ggtttttttc agcatttgca ggtcaggttt ggtgacgggc cgtggcagga
      421 tgtcaagggg ctggatgaag tgggcagtga tacaggcaga acaggagaat gacatgaata
      481 ttttgagaaa gcttatgcag agtctgtgcg gttgcggaaa gcatgatgac tgtgaaaacg
      541 ggcagtcgct tacagcacaa ctgcgactgg gaccggcaga cattctggag tcagatgaga
      601 atggcattat tccggagcag gacagggtaa tcacacaggt ggtgatactg gatgcagata
      661 aaaagcagat acagtgtgtg gtaagaccgc tgcaaatcct gcgtgctgac gggacgtggg
      721 aaaatattgg cgggatgaaa tagccgacag cttcacaaaa accggagccc ggctccggtt
      781 tttgttgtca tgtatagggg gggttattag agagtgaagt aataaacatg ttaatacgat
      841 ggagtgaagg atgccgtgta attctggttc aagagttttt tatgccggaa aatcgcagga
      901 ttattctgga tagtaaagaa tcctggttaa taatctgtga tagtcagttg ggccatttaa
      961 tgcgcagtat gtatcaggga cgccgtttta ttcagctgaa tctggaaaaa ttgaaagggg
     1021 tacatgatgt cgccttgcca gtgaaatggg aattcacacg aagacagtga atagctttct
     1081 gtatacggga atggcgaaaa atggactgta tggtgtgagt gtgaaacatc ttgcgtgtgc
     1141 ggagtgatgc ttctcgttgc tacagcggca atgataatgc agtgaaaaaa ggggagcaat
     1201 atgctccccc aaaccgaaag aaaattgcaa taatcaatga agttatttag tcatcatcag
     1261 aatgtcatgc aaggcatttt gtttcagtga tgccgatcgc gattttagcg aattccatca
     1321 taaatcccct gatttttaag cctgaagcag tcaaaggaat ttctatgccc tatatcgata
     1381 tcaccacgat gcgtgggatg atgccgcgcg ttgtgacatc catgctgccc gagcattccg
     1441 ctgtactggc ggaggactgc catttccggt ttggtgttat tacaccagaa cgtcagatat
     1501 ccggggttga gaaaacattc acaattaagc caaaaacaat ttttcattac cgtgacgatt
     1561 tctggtttgc atggccggat gtggtggatg tgatccgcag tccgatcgct caggaccccc
     1621 acgggcgtat ttactacact gacgggcgtt ttcctaaagt gacggatgcg actattgcca
     1681 caaaagggga cgggaatcac ccgacatcat cgtatcgtct ggggatcccc gcgccgacga
     1741 cagctcctgt ctgtactgtt cagcagggcg gtgatgtttc tgacgataac ccgaatgatg
     1801 acgaaacccg gttttatact gaaacctttg tctcagatta tggtgaagaa ggtccgcctg
     1861 gtccggcgtc tctggaggta acactccgta ctccggggac tgcggtacaa ctgacgctgg
     1921 ctccggtgcc attgcagaat gccaatatta aacgtcgccg gatttatcgc tctgcatcag
     1981 gtggaggaga agcggatttt ttacttgtgg ctgaactgga tgcgtcagtg ctcagttaca
     2041 cggacaaaat accgacgaaa aaccttgggc cttccctggc aacatgggat tacctgccgc
     2101 caccggagaa tatgacgggt ctttgcctga tggctaatgg tattgctgcc gggtttgccg
     2161 gtaatgaagt gatgttttcg gaagcgtatc tgccgtatgc atggccggaa gtgaatcgtc
     2221 acacgacggc agaagatatt gtggctatct gtccgctggg aacgtcactg gtggtggcga
     2281 caaaggggga gccttatctg ttcagtggtg tatcgccttc cacaatttct ggctccagaa
     2341 ttccttccat gcaggcatgc ctgagccgaa gaagcatggt ggcgatggag ggattcgtac
     2401 tgtatgccgg gacaaacggt ctggtatctg ttgatgtaaa cggtaataca gcactggcaa
     2461 cggaaaagat tatttcacca gaacagtggc agagtcagtt taacccgatg tccattgtgg
     2521 cttattcctg gcgtggtgac tatatcggtt gttacacaaa accggatggt aagcaggatg
     2581 tgtttgtatt cagtccggcg aacatggata tccgttatct cagcacgccg tttgactgtg
     2641 catggattga tcttgcaaaa gatatgatgc gcgtggtgac aggggacaaa atgtcagtgc
     2701 ttgccgggga ctctctgccg tccatgataa ggtggcattc aaaaattttt tcattacctg
     2761 aaagaacctc tttttcctgt atcagagtga aatctccggc acctgagcgg gtggggatca
     2821 ctgttatggc tgatgatgtt cctgtgattc attttgcgcc gggtacgttt aagggaagtg
     2881 tggtgagact tccggcagca accgggcaaa actggcaggt gatggtatcc ggattcgggc
     2941 aggtggaacg aataaccctg agtacatcga tgtcggagat gccggtatga ccagaaaacc
     3001 gtggcgtgcg gggaaggatt tatccacagt tgtggagaac atggaaattg gcaccgggca
     3061 gcgtggtgac ggacgccacg catttgtgac ccgtgaggaa ctggttggtc ttaaactcgc
     3121 ccggcgtcga acatcgggtg gtgcctcata tgcactgaat ccgggtattg agattgacag
     3181 tactttaatg actgttgatt ttcccacaaa accgctgaat tttaaggcga caggaggatt
     3241 tggctcggtt cttcttgaat gggatatgcc taattatcgc ggacattcac tgactgaaat
     3301 ctggcggggt acggaggatg accttgctga tgcagtgctg gttgccacga cgccggggca
     3361 ggtttacggc gatccggttg accctggctg gtcgggattt tactggatac gttttgttaa
     3421 cgcggcagga gtgaaaggtc catggaatgc tgaaaaaggc actcaggcac aaacacagat
     3481 cggcgtgaag gccatcattg accagatccg cgatgaggct gcaaagtcgc cggttgtgtc
     3541 cgagctgcgt aaagaaataa aaaacgcgca ggggcaggct gtaaaggatg ctgcaattaa
     3601 gacaaccgaa gttgtgggga ctctcaggga agaaacgaca agaacgattg gtggtattga
     3661 aacccgcatt agcacactgg attcgtcaac cagtgaatcg cttaatgagg tcgacaagcg
     3721 catcactaaa ctggataaag aaggcggtga ggcgtttctg gcaatgtggt caaaaaaagc
     3781 gggagttgat ggtatcactg cggggatcgg gattgtcgcc ggaaaagaca gtgaaggcag
     3841 gcctgtaagt caggttgcaa tttctgcgtc gcagttgttt gtctttgacc cgaacaaccc
     3901 ggataacacc gcctatccgt ttgcggtatc aggtggcaag gttgtgatcc cgaaagcgat
     3961 gatttatgac gcggtgattg aaacactggt gtcgcggaag gttgtggcgg atgaggtaaa
     4021 agccggggta agtatcactt cgccagttat ccggagtgcc gttattcaga acggaaactt
     4081 tcaggttgat tctcagggta acctgaatat tggaggcctt ttcagtgtta cgtcacaagg
     4141 gcaactgaca attcgttact ctaatcagaa tgtaggactg gtgatccgca atgataaaat
     4201 tgaggtttat gatcagaatg gacgactggc tgttcgcata ggcagattac gctgatcagg
     4261 aggtgagtat tggaatacgg ttttgccatt tataacagaa ataacgttaa tgttacgggc
     4321 gtgctgactc cggtattttt cctggacaga tttacagcgg agtctggctc aaagacgtac
     4381 acgaataaac ccgacgggaa atcattgcag gctgtatgtt gtttatttcc ctggaataat
     4441 gtatttgcgg atcggaaagt accgaagata accattaatg gcaatacggt gacgtggtcg
     4501 aatcttgagc agggtatggg atcttatatt tatacattct ggggataagt gtcatgtatg
     4561 gtttgagcat tatgaagccg gatggcagcg tatggataag tccaggtttt acgccgcagt
     4621 gtctgatcaa caaaggcacc ataccggcga ctgaaaagtc tttttttaaa acatcaatcc
     4681 cgtcaggcaa aagttgtttt ttctttatca gaacagagaa gaaggccgat gtcatgtaca
     4741 cgcatgaaca gattgatgga tatcatgcac taaggcttca tgtaattgtc aggggaacga
     4801 accctggtgt tacgacggtt tatgctttcg cgaatatggt tactccacct tctgagtatg
     4861 gtatcgccat gtataacccg gacggtgaga tgatttatca tggcgaaatg atgctgcttg
     4921 acgcgaagtt aatacctgtt gatatcaaat ttgaaaagga ccttggatat ccatgcgcaa
     4981 tcatgcctgc actggtcggg tattataact ggaaaagaac tccttatgat cgaccgattt
     5041 ataccacatc cactggtgct acaggaaata aaatatattc ctgtgagcat tattccggcg
     5101 gtgcaacatg ggatattcga aagccgtata tagataaggt cttggttatt aatacatcag
     5161 tatatgatta gttgaagcga gtctttaata ttcatttaaa atgtctaaaa agatgtatta
     5221 ttaaaaagtt tagcgtgtta tctgaataca ggatatctta aatgaagagt atagcaacac
     5281 tggttgtgtg tgcaatctcc gggattgcct gtgtaaattt atctgcacat gcagcagaag
     5341 gaaaccatac aatttctctg gggtatgcgc actttcagtt tccgggactg aaggattttg
     5401 taaaggatgc gactgctcat aacagggaga ctttcagtca tttcgtcaac agaaactact
     5461 tttcttcatt gggcgaatat acagatggtc gggtcagtgg atatgaaggc aaggataaaa
     5521 atccacaggg cattaatatc aggtatcgct acgagataac ggatgatttt ggcgttatca
     5581 cctcttttac atggacgcgt tctctcacta actcacagac atttattgat gtgcagtcag
     5641 ccgatcatac caggaagatt aagaatccgg cagcttctgc cagaacggat atcagggcga
     5701 attactggag tctgttagcg gggccttcat ggcgggttaa tcagtacatg agcttatatg
     5761 cgatggcagg gatgggcgtt gctaaagtta gcgctgacct gaaaattaag gacaatatta
     5821 acagtagtgg cggattttct gaaagcaaca gcacgaaaaa aaactccctt gcgtgggctg
     5881 caggtgcaca gtttaacctg aatgagagtg ttacactgga tgtggcttac gaaggttccg
     5941 gctctggcga ctggcgcacg agtggaatta ctgctggcat tggcctgaaa ttctaacctg
     6001 tatccggtaa ccgtttacta cccgctgtga tggcgggttt tttattgccc gtacagggca
     6061 aaaaccgtaa attatgcgtg ggtgcctttc ggctgatggc tggagggtga acctgaaggc
     6121 ctgatgtgga aaggccccga gtcaacttaa cgttaacccg aggccctaac acttcgtacc
     6181 ttgacaagta gaaggttagc gcctctccac tcaggaggca agcgctatgt cgcaaaaatc
     6241 gcttatcacc gtcacaattt gtatgacggt tatcttcacc atctggatgt tacacggctc
     6301 gttgtgtgag ttccggctga atttgtgggg agcggagttt gcggcgttct tacagtgtaa
     6361 gcagtaggaa aaccgcggcg gggacgaaag tccccgtcaa ctggttgctg aggttcagcc
     6421 gatatggcac ccgtttcagg tgagagaatg aacgataaaa ttctccggta tatgcagcgt
     6481 gtggtgagaa attcccgcaa ccctgaattt atgaatgaag ttaaagacgc ctgccttaaa
     6541 aagcaggcgt tttgttttga ggcacctgat ggctttctgg tgctgcgttc tgtgctcagt
     6601 gctgatggta tcccttatgt tctggtgttg ctgggcgtgt gtacggggag taacagtgtt
     6661 gagcgttatc tgccggaggt gaagacatta acccgtctgg ctggcggacg ttgggctgaa
     6721 tttcatacgg caaggcgggg atttatccgg ctgggaaaac gtctgggctt tgagcgaatg
     6781 ccggatgatg aggatggctt catggtgttc aggatagcgg tctgactgcc acagttttca
     6841 tcatcgtgtt taaaccaaca ttgcaattca cattctgacc ctgctccggc agggtttttt
     6901 tattatccag ggggccatta tgggtggaag taaaggtggt ggtgatacca aagtaaaacc
     6961 aacagcagcg caaatagcac aggaagaagt ggcctggaaa gggtggcagg attacaaaaa
     7021 tatcctccgc ccggctgaag ataacttcat ggaaaaggtc gatgacctga acagtgagca
     7081 gcagtacgac aatattgctg gcaccacaaa tctggggtat cagaaacagt ttggcgaagc
     7141 acggaaggag ctggcgggta atcttgctca gtccggtgtt gacccgtcca gtggtcgctt
     7201 taacgcggta atgaatgcga accagagtga tcaggtaacc gggcagattg acacaaccac
     7261 acgggggcag gtatcgcagg cagataagta tgttgccggg ctacaggatg ttgcagctct
     7321 cggttcaggt cagaaggcgg atgcgttaca gagttttaac tcgctggcag acagcagtct
     7381 ggcaaaagct aaatcggacg cacaggctgc gtttacgaaa cagcaggggc gagcctctct
     7441 tgttggcgct ggtttgggtg cggtaggtgc atatgcgatg cataaggctg gtggtagcgg
     7501 aggaagtggc ggtgctaaaa cacctggcac cggcgctaat gccattcagt atcaggctca
     7561 gaactggaga ctgtgattat ggagtacggt aaatacgaaa cacttgcgag atacggttat
     7621 accggagcag cccgccctca gggggactgg cagacatccg cagcgctgac ccgccagcaa
     7681 tacgacgact ggcgcaccag atatttaccc cgtgtagcaa ggctggctga ccttggcgag
     7741 aacaacagcc tgatgaatgc acagcttgcc cgggtgggcg gccttgccac ttccagtctc
     7801 cgtacagcgc agatggcgca ggataaccag atggcgagat acggggtaag ccgcccggat
     7861 aatcccgaca gtaatacgct ggggttacgt aatgccctgg caattgctgg cgcgaaaaat
     7921 ggtatccgtg aagccgaaca ggatcgccag atgaatattc tgacgggggc ttctgcaccg
     7981 gcaagacaga aactgagtgt tggcggacaa ctggtggcag cgtaaggagg aaatatggga
     8041 tacggtttac tggatattgc aaatcagtcg cggcgtgagg cattacaggg aataagcgag
     8101 gcagacagac gacgtgaaga aattgaggcg tcaaacaaga tgatggcggc gcagcagaaa
     8161 gctcagaaaa aacagaatat cgccacgggc attggtacgg gggctgctgt gggtgcatct
     8221 gtggcaggct ccagcgctgc aatgggggcc acgctcggag catgggctgg tcccgtcggg
     8281 atagctgctg gtgctgtgat tggcggcatt gcaggcgctt tgttttaagg agtggtgaat
     8341 gagcggattt gcacaggggt tacttgccgg attcagcacc gttgaccagg caatgacccg
     8401 tcgtaaggag cttggtttgc gagaagcaca gcttgctcag caacagaaaa ataacgagcg
     8461 cgattttgaa tttgcgcagt cgcagtttga gcaccgaaaa gagaatgatc agcgtacata
     8521 tgatcttaat gtcaggaatg ctgaccgtga atatgcgctg aaagagaggg aacacaaagc
     8581 agcgcagaat tatcgaaatg cgtcacttgg gctatctcag cagagactgc aactggaaaa
     8641 atacaaccag cgacggcttg agtataacga tatgattgcc catagccagc cactaatgga
     8701 agcgcttgga aaagcaattg aggctggcga tcaggaggct gcaacgcgtc tgttcgggca
     8761 gctgccaaag ggacatccat taattcttat gtcaaacgaa ggctatgcag cgaaagcggg
     8821 tcaggccgtg atcaacctgc aaaaaatctt tggtgataag ccggacatgg cgatcgattc
     8881 gctgaatacc ccggaaaatc tcgatgtgct ttccggcgtg tttgccccgg aactacaaca
     8941 gcgtattggc atgcctgatt caaccgggga aaaaacgata aaagaggcca ggattggcag
     9001 tatcgtaccg gcgcagcagg aagggtacgt acttattggc cttgatctca catacagcga
     9061 tggctccacc gcgcataaac ctgtaacaga atacggcagt gcgcaccctg atgatcaaac
     9121 cgtgctggcg atacccgttg ataaggctat cgctcaggtc agggatcgca gcaaatttgc
     9181 agagatatcg aaaaattatg gttattttat gccgaagcag cagggacttt ctctgaaaga
     9241 gcttcagaag ggggccagca acgtagcggc ggacgcgatc aagaatggcg gtaatgcaca
     9301 ggctgcggtg gatgaatatt atgctgcgac tggttcacaa ccgcatcaac agaaaattca
     9361 gcaacaaaaa cttcagcaac aggttatcaa ctgggcggga gatgatcctg ataagctgtc
     9421 atttgccaga aatgtagcgg cccgtcagcc tgaaatgctg gagcctcaga atcagaaatt
     9481 gctggagaac gggtatgcga attttctccg tattcaaaag gctagggggg aacaggccag
     9541 agatgaaagt gcttcatctg catctcagtt tatccgtgga ctgaaacaga attacgccca
     9601 gtaattcacg atattccatt aataccattt cctgatgccc ggccattgtg ccggtttttt
     9661 tatggagtct gtatggccta ttcagaggaa cagcgtcctg aggcgcaact cggtaaccag
     9721 aatcgtaaca gcctgaacat tcagcaaccc ggcgaaactg acagctatga agcatttttc
     9781 tctgatccga atcgctggaa ggataacagt acgtcgttca gcctgggcga tgtattgcca
     9841 acaatgggta aaggtttcgc ccagtccgtc cggggaacag gggaaatggc ccgtggactc
     9901 ggtgatgcga tgattcagag cccggtaaaa acaggggcgc gtattttaaa tgagttcagc
     9961 cgtatggggc tgccgggtgt cgcaactgtg caggatattt ttgccggtgg cagcaggggg
    10021 gctgatgagg tcatcgatac cctgcctgat ggcaaaaacg cggttactga tactgtcggt
    10081 aaaggtctga aggcaaccgg taaggctgtc agtgatggtg ctaaagccac tgatgaatgg
    10141 ctgaccggta agatgtcgcc gggtgcagtt cgtgcgctga atacgccgat gaccgaaggc
    10201 tatgatgatt ctgcggtctg ggtggcgaag ggtgtaaacc tgattggtgc gcttgtacct
    10261 gatatggttg ctggcggtgt ggctaaaaag gtgggtgatg tcacactgcg aaaaatgctg
    10321 accgccgggc tggagaaaaa atacatcgcg gcagggatgc agccggaaag agccacggca
    10381 ctggcagcag aagctgtcga taaaaaaatg ccggatttat tccaggcggg cctgatcacc
    10441 cattccacag ccagtgcaca ggggcagagt gcaatggcgg cagcagatgc tgttcttaat
    10501 gcggattact ctgagctggc gcagtcaccg aaatttcagc agacgttttt gtccattgac
    10561 gccgacccgc agcacgcaca gcttactgat cgccagaaaa tggatctggc aaaagagcgt
    10621 gttgccgatg aggtgcgcgc gcagctggca accgatcctg aattgctggc tgtgaatgcc
    10681 atggcggcaa aactgggtga cgcacaactg tttaatctgg tgacacgagg cacagcgaag
    10741 accgttaaaa gcggcattgt cagaaatgcc acggaacagg gggcgattaa tgcggcgcag
    10801 ggcggctatt cacgctatca ggaaaacacg gcattgcgtg agaccgccgg aatggatgtg
    10861 tcaccgtggg agggcgtggc tgacgcaacg atcgaaggtg cagcctttgg tgctgcgatg
    10921 ggggctccat ccggtgcggt tgccggatat cgtggcagac gtcaggccgc agaagaagca
    10981 gccatgcgtg atgctgaaac cgtgcagcag gacgacgcag ccccgcaacc agaatctgtt
    11041 gatccggtgg cgcagcatcg tgaatccatg caggggatga atcgcgagca gcttctggag
    11101 cagtatgctg atgcggatat ggcaacagag ggtgacgcat ccgcagctca tcgccgggaa
    11161 gctgccagcc agctgttgaa tgaactggat gaacaggcga agcgacaggc ggtgatggat
    11221 gagctgaagg cgaagccgcg tcctgaactg cttgaggaat accgcagact cagcctgaag
    11281 gaagggcgta ctgagactga agagcagcag ttacaggcta tccgtgatgt gcttcgccct
    11341 cagcaggagg ccagaccgga ggcacagcca gaaaatacag atgatggcga cgggagtatt
    11401 tatccgacgg tgcggttccg cgatccggat gaagtgcgta ttgaaattaa cgggagtggc
    11461 gcgtccagac cagcggaacg cattgaaaag gtgcgtccgg acaaccgtta tttcacggat
    11521 gagaaaagcg ctatgggtag cgatgttttt cgcaatgcca gcgccaccgg cctgaaaccg
    11581 tccgtagtga agaaaggtga gaatcagtat gccgttgaaa tggataatcc tgcgttctct
    11641 gaagatgtgg caacggaaac cattaacacc ctggctgacg gagagcgtat tactgatgct
    11701 gatccgatgg agcagccagc gttcatgcgt gacccgcgat tccggggttt tacgggagat
    11761 gatacagaag tgcaggcccg ccttgcccgt ggcaacgtgc cgacggcgga agagctggtg
    11821 cgttcacaga tggctgaagg tgatgccggt ccgacagctc aggagttaac tgagcgtccg
    11881 cgtctgcccg cccccggcga tattcatccc ggacagggat atccgttacc gggagaagtg
    11941 gcgcgtacgc cggatgaaaa tcaggccgga cgtggtggtc gttttaccat aaccggtgag
    12001 gttaagggcc agagtttcca gaaaggacgt gctccggcac cggaaaacgc cgctggtcgc
    12061 cagggggaaa cactcgaggg tgaaacggtt cgtcggggtc tgccgtcacc ggatgcgcag
    12121 aacgcgacag caccggtacg tgaagggcta ccggctcctg acattgcgcg taatgttcgt
    12181 atgcctcagc ctgaatcact tccccgcact gtacgggact cactgcctga gctcgcacag
    12241 caggcagaag tacgtcggca ggccagagaa aatcgtgaca tcctgcagcc tgagacaatc
    12301 gcacctgaat ctgaaacaac agtctctact gacagggaag ctaccgtgcg cggaggtgaa
    12361 gtcaggggca aaaaaattga agactttggt gaagaaatta agggcgcggc aaaacaccgt
    12421 tatgcacagc ttgccgaaac actgggtaaa acgctggaag acagggatta tgtcacgcaa
    12481 ccgctgagca aactgttccc gaaaccggac tacgcaaaac tggcgaacga aggtgccgat
    12541 gctgataccc tggcaatgat agcgctgtat cgtagcgata ttccggcgaa gacgaaactc
    12601 aatgcagtga agtgggtgga gagtgtaaag agtatccgca caagtgtggc gggaatgctg
    12661 gaaggaaaag tgactgccgg acggcttgct gagtggatgg aaggcagaat gcccgcccgt
    12721 tacgcggaca catggcaact gttacgcacg ctgccaccct cgcagataga caaggcttct
    12781 gcttatcggg tggtatccgg tgtgtatcag gcggcaggag ggaaacgtta cgatccgcca
    12841 cggaaacttt attcactgcg caataaggac aataagggga gcaatctctt tttctcggaa
    12901 agcagggatg aattactgac aaaggcaaaa gcctggtttg cagagcagga ggaaaaatca
    12961 caggcgaaag gtgatgaaaa aacagcactg tcaccggatg acaaaatcag ctttgacgta
    13021 taccggaata cccgcagtgg cgatattttt attgcttacg gcaaaaacaa aatgcgggtg
    13081 agaggtggct ttaagtcagc cagtgatgcg cgtaagtaca ttgattcaca tcgtgatgag
    13141 cttgttcgtc atgtgaagga gatgcgtgag atttcgcgtg aggagcagcg taacgccacc
    13201 aaccgcgatc gtaccgggcc agaacgccgc aagggtgatg tctcaccgaa gcagttcagc
    13261 gatgcgtttg gttttcgtgg tgtgcagttt ggtaactacg tggaaggtcc gcgtcgtcag
    13321 gctgatttga accgggctta tgactcgctg catgaccttg cggaagtact gaatgtaccg
    13381 acaaaagcgc tttccctgaa cggtcgtctt ggcctggcat ttggtgcccg tggtaagggt
    13441 aaggcggcag cacactatga gccgggtgag gtggcaatca acctgacaaa aggtaacgga
    13501 ccgggtgcgc tggcgcacga atggttccat tcactggata attattttgg tcgttatgat
    13561 gtttccactg acgggaagat tacgtcaggt ggtgacttta tgacggaagc caggcgtgtc
    13621 aagcgcgtat ttaaagacgg ccggtttgtt gacgctgaat atccggtgcg tcaggaggtt
    13681 tacgacgcgt ttaaaggtgt cattgaggcc attaaaaaca gtgacatgcc gcgtcgttca
    13741 gcgcttctcg atgaggtgcg ctcaaaaccg tactggtcaa cggatgttga aatggcggca
    13801 cgtgcctttg aacgttatgt tcaggataaa gcgcgtaaag ctggcgtgga gaatgattat
    13861 ctggtcaata tccgtaaggc acctgagcac aacacagata acacctacgc ttatccgacg
    13921 aatgcggaac tggatggcgg tattcgtgag gcattcgatc acctgttccg caccctgaaa
    13981 acccgtgaga cggacaaggg cgttgcgttt tattcccgta agggcgttac ccgcacacct
    14041 gaaggtaatc tcatttcgga tgttaaccgt agtgcggaag ccaaaggcag cccggtcccg
    14101 caggttgaag cggttgcccg tggcgtgatg agcggcatta aggacagtga cctgaaggtc
    14161 cgtgtggtga agtcacagaa agaggctgaa gcgctggcgg gtgaattgtt cgatggttac
    14221 ggcagggtgc acgcattcta tcgtccggat aaacgagaaa ttgtcctggt ggcggataac
    14281 atccctgacg ggcggaccgt tcgcgagaag ctgcgtcacg agatcattca ccatgccatg
    14341 gagcatgttg tcacaccagc ggaatatcag acgattatca aaaccgtgct gaaaacccgc
    14401 gacagtgata acgtcaccat ccgtgaagcc tggcgtaagg ttgatgcttc ctatggtaag
    14461 gaatcaccgg aagtacaggc gggtgaattt ctggcacata tggcggagaa acagccgaat
    14521 aaattcgtgg cggcatggga gcgtgttgtt gccctggtca aaggggtact gcgtcgtacg
    14581 gggttactga agccgacgga actgaacgat atcagacttg ttcgcgagac catccgtacg
    14641 ttaggccagc gtgtgcggga aggttacacg ccgcgtgagg atggcgcggg cgcatcgtct
    14701 cagtactccc gtagtggtaa acgtgatccg ttcaaagtgc cggaaggtga gggcgagcgt
    14761 tatcgtgatg accttgccag aatgatgaaa tctctgcgca ccacagattt aacggtaaac
    14821 atcgggcgta cgccgccggt attgcgtcac cttggtgcac cggatttgcc gctggttatt
    14881 tcccgcgata ctgtgcggaa ggccaccaat ggtgtgaaac atgtggtgcc gatggatgtt
    14941 atcgagagac taccggaact gatgcacgat ccggatgcaa tttaccgctc agcgacagaa
    15001 agaaatgcgg ttgtgatgct gcttgatgcc gtggataaaa atggtgatcc ggtggtgtca
    15061 gcggtacaca tgaaggctgt ccggtcgcgt ctggaaatca acaaggtagc ttctgtttac
    15121 ggtacagaaa atggaaaaaa actgaagagt atggaaatga ccgggttaac gttgtaccgg
    15181 agagaaaaat taagccgcga taaccttctg cacagagggc tccaattgcc caaaggggaa
    15241 cattcttatc gcggctctgc ggataaaata ctctatcctg aagatattcg caaggggccg
    15301 tattactccc gtaccagcag tctgacaccg gaagagacaa ttgcatcgcg ttttgtgcgc
    15361 cagatgcagg ataaattcca ggtgctgaaa gctgttcagg agaatatccg taaaactggc
    15421 ggcaaagtgg acgacagtaa caacgcttat atggcggaag aactcttcca cgggaaggcg
    15481 gaaaacgacc tgaacgtgat gaaggagcgc tacgttcagc cactggctaa attactggcg
    15541 gactacaaaa ttgcgcaggc cgatctggat gagtacctct acgcccgtca cgcgccggaa
    15601 cgtaacgcgc atatcgcgaa aatcaacccg aaaatgccgg acggcggttc ggggatgacc
    15661 aacgcggaag cggcggaaat catgcagcgt gtacgtaaca gtggcaaaca ggcacagtat
    15721 gaccgtctgg cagggattat tgacgatatg ctggcccgtc gccgtgagct tatccgtgag
    15781 gccggacttg aagagaacgg tgtggtggat gcctggcaga acgcctaccg ttactacgtt
    15841 cccctgaaag gtcaggatgt tgacggtgtg gtgtcactgc cccgtacagg taagggcttc
    15901 accatcggcg gacgtgaaag caggcaagcc atggggcgtg catcccgggc acagtctccg
    15961 tccactcagg cgatacagga cctgagcgaa tcgctgatcc gccatcgcaa aaacgaagtg
    16021 ggtaacgcct tcctgaaact ggtgcaggat aatcccgaca aggattactg gcaggtattc
    16081 accgatgaca gaccggatac catgcggacg attgcagagc gcaaggacca ggaaactggt
    16141 gaaaccattc gcgaagttgt cgaacgccct gtaccgatgg caatgatggc agaccggtac
    16201 ttcaccacca aaaagaacgg caaaacgtac tacatcaaac tccatgatcc gcgcctgatg
    16261 cgtgcgatga agaatatggg accggaaacc agcaatgcct ttgttcgtac gctggggaaa
    16321 gttaaccgct tcctggcaac ggtgaacacg tcgtataacc cggaattcct ggtcagtaac
    16381 ttcatccgtg acgtgcagac ggcggtgatg aacctgaagg cggagcaggg aaggagcgac
    16441 ggtaaactga aagggctgga taacttatcc gccctggctg tggtgaaaga cagccgttct
    16501 gccatgtcag ccgtatacgc cagtctgcgt ggtaaaaccc tcacgggaaa aggtgcacag
    16561 tggcagaagg tgtggaaaga gtttgttgag gacggaggta aaaccggctg gtttaacatg
    16621 ggtgaccttg aaggccagca gaaggaaatg gatcgccttg tatcgctggc gaagggagga
    16681 tggaaaggcc agagtatcgg tgcatggaat tcgttcctta accttgtcga ggatgccaac
    16741 ggggcggttg aaaacgctct gcgtctttct gcctataaac acgcccgtga tgccggtttg
    16801 tcacgccagc aggcggcgtc tcttgccaaa aacatgacgg tgaactttaa ccgtcgtggt
    16861 gagcagggag cgctgatgaa ctcgctgtac atgttcgcca acgccagcat tcaggggacc
    16921 gcaaacctgg tgagaacgct cggacatctt aatggcgacg ggccgttact ggagcgcctt
    16981 cgctggaaga atctcaatgt accgcagaaa atcgcgcttg cagctgtggg agcgggttat
    17041 ctgcttggct cgcttaaccg cagtgttgcg ggggaggatg atgacggggt taactggtat
    17101 gacaaggtgc cgtctcatgt gaaagagcgt aacctcgtca ttatgaaatc ggtgttcggg
    17161 ggcaaggccg gagagtactg gagtattcct ctgccttacg ggtacaacgt tttcttcctg
    17221 cttggtcaca cagccgaagg ggtggcagcg ggcgacctga ccgcttcacg tgctgccggt
    17281 aatgttgttg gtggtgtgct tggggcattc agtcctgtgg gcagtgaaac gtcggaaaca
    17341 ctgtccgggg cattgctgaa aaatgcagca ccgacaattc tgcgtccgtt tgcgaatctt
    17401 gccatgaacg aaaacttcat gggggcgcag atttaccagg agaacatgcc gtttggtaca
    17461 ccaaaacctg acagccagct gggaagacgt tcaacgccag aagcgtacaa ggcgtttgca
    17521 tcctggctga atgcgttctc tggtggcagc cagtaccgtt ccggtgcggt ggatgtcacg
    17581 ccggaatcac tgaaatactg gattgactat atctccggag ggacagggcg cttcatttcc
    17641 aaaaccacgg atgcggcggt gaaatcgctg aacggtattg atataccgga gcagcaggtg
    17701 cccttcctgg ggaaaatttc aggtgaggtg atgccgtatg ctgaccagca gaagatgtac
    17761 gaccggatga cagaggttgc gcagtatcac gcagagctga agagtctgac cggtgcagaa
    17821 agaacggcgt tcattgacga gaacaacgga aaattgtcga tgaacgggct tatgcaggat
    17881 acccggaaga gactgaagga tttgcgtaaa cagcgtgatg ccatttacgc cgacagtact
    17941 ctcagtctgg cgcaacagtc ggcgatggtg aaatcggttg agcgggatat gaaaattgcc
    18001 gtggatcggt ttaaccgcga gtacaacaaa aaagtgggag tggagtaaag aacatgcccc
    18061 gtacggaagt gcggggcatg cttaacgggg tgtgtacata aatgggtact gatatgacga
    18121 ttatttcgcc actttctgta tttaaatcaa tgacttaata cttatattca ttatatccat
    18181 ttaactaaga ggacaatgcg gcatgagtat acccgctaat ggactgcggg gtaagtacgc
    18241 tgccgctcga ttgcttaaac cctcgccatt tatgccgggt ttttatcatt tttcttaatg
    18301 ttttccgcac gttctgcttt ttggcgtgct tctgctttgc gcttgttgct catgtcgtta
    18361 cggatctgtg catgactcat taacgcgaag ataaaggtgc cgccgcagat gttccccgct
    18421 aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt gcagcgtacc gttaaacacc
    18481 agatagagga tttcaacaga accgacaacg atgtgagtgg tgtcacccag ggcaataagc
    18541 caggtcatca atataatcac cacaatcttt gccgcacccg ctgcaggaaa catccatacc
    18601 atagtggcga tcagccagcc ggaaatgatc gcgttggcaa acatctcgct gggggtgttc
    18661 ttcatcacat ccatgccgat tttgacaaat gcatcgcgag tttcttcatt gaagattggc
    18721 atatattcaa atgcccacgc cgcaatacct gtcccgagaa tattacccag cagcacgacg
    18781 ccccataacc gcataagtaa gccaacgttg ctcattgtcg gtttttgcat gacgggtagt
    18841 accgcagtca cggtgttttc ggtaaataat tgctggcggg ccataatgac gataataaaa
    18901 ccaaaggtat aaccgagatt ctccagcaag aagctgcccg gcacaccttc cagttcgaca
    18961 tgaaatatcc cttttgccag taatgaagcg cccatcgaca gacccgccgc aatggctgac
    19021 cacagtagtg ccattgcgtc gcgttccagc tctttttcac catcctggcg gatatgctca
    19081 tgaattgcca tcgcccggga ggggagtcga tcttcatcta tttctatttt tttgccacgc
    19141 tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc
    19201 atggtctctt ctcttgaatt agcacgtata gctaaagcgt agcggctttt ttgctcgcaa
    19261 ctggcgggag ttactttgaa aatgtagaaa aggccgcgtt tgcctttttc tgtttctata
    19321 gaatcaagta gcctacaggg cggcgattac caggctatga tcaaatcagc aaatcagggc
    19381 gtctggacat cagttgacgt gctgttacaa tcgcccacac ctaaacaggc ggatacggta
    19441 tcgttccgtc atggatggca aactgcataa gccataaaaa aacagggaga catttatgaa
    19501 gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg cgagttccgg
    19561 tacagatcag caagggcgtt ctgacccgtt agaagggttc aaccgcacca tgtacaactt
    19621 taacttcaat gtattagacc cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta
    19681 tgttccgcaa ccggcgcgta acggtttgag caattttacc ggcaaccttg aagaacctgc
    19741 ggtgatggtt aactacttct tgcagggcga cccttatcag gggatggtcc actttacccg
    19801 ctttttcctg aacaccattt tggggatggg cggctttatt gatgttgcag ggatggcgaa
    19861 cccgaaactg caacggactg aacctcaccg cttcggtagt acgcttggtc attatggcgt
    19921 gggttatggc ccgtacgttc aattaccgtt ctacggtagc ttcacgctgc gtgatgacgg
    19981 tggtgatatg gcggatggtc tttacccggt tctttcctgg ctgacctggc cgatgtctgt
    20041 gggtaaatgg acgcttgaag gtatcgaaac ccgcgcacag ctgctggatt ccgacggtct
    20101 gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga
    20161 tttcatcgct aatggcggcg aactcaaacc gcaggaaaat ccgaacgcgc aagcgattca
    20221 ggatgattta aaagatatcg
//
LOCUS       DBFIFS010000063        20139 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.063, whole genome shotgun sequence.
ACCESSION   DBFIFS010000063 DBFIFS010000000
VERSION     DBFIFS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.063"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(131..889)
                     /gene="aroD"
                     /locus_tag="N7A07_002120"
     CDS             complement(131..889)
                     /gene="aroD"
                     /locus_tag="N7A07_002120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU2476781.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(920..1786)
                     /gene="ydiB"
                     /locus_tag="N7A07_002121"
     CDS             complement(920..1786)
                     /gene="ydiB"
                     /locus_tag="N7A07_002121"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU2476782.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1798..3063)
                     /locus_tag="N7A07_002122"
     CDS             complement(1798..3063)
                     /locus_tag="N7A07_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476783.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3290..4504)
                     /locus_tag="N7A07_002123"
     CDS             complement(3290..4504)
                     /locus_tag="N7A07_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476784.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4604..4960)
                     /locus_tag="N7A07_002124"
     CDS             complement(4604..4960)
                     /locus_tag="N7A07_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU2476785.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5096..5203)
                     /gene="rprA"
                     /locus_tag="N7A07_002125"
     ncRNA           complement(5096..5203)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="N7A07_002125"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5389..6501)
                     /gene="ydiK"
                     /locus_tag="N7A07_002126"
     CDS             complement(5389..6501)
                     /gene="ydiK"
                     /locus_tag="N7A07_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU2476786.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            6890..9946
                     /locus_tag="N7A07_002127"
     CDS             6890..9946
                     /locus_tag="N7A07_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU2476787.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9943..10353
                     /gene="menI"
                     /locus_tag="N7A07_002128"
     CDS             9943..10353
                     /gene="menI"
                     /locus_tag="N7A07_002128"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU2476788.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            10453..10641
                     /locus_tag="N7A07_002129"
     CDS             10453..10641
                     /locus_tag="N7A07_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU2476789.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11190..11558
                     /gene="sufA"
                     /locus_tag="N7A07_002130"
     CDS             11190..11558
                     /gene="sufA"
                     /locus_tag="N7A07_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU2476790.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11567..13054
                     /gene="sufB"
                     /locus_tag="N7A07_002131"
     CDS             11567..13054
                     /gene="sufB"
                     /locus_tag="N7A07_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU2476791.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            13064..13810
                     /gene="sufC"
                     /locus_tag="N7A07_002132"
     CDS             13064..13810
                     /gene="sufC"
                     /locus_tag="N7A07_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU2476792.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13785..15056
                     /gene="sufD"
                     /locus_tag="N7A07_002133"
     CDS             13785..15056
                     /gene="sufD"
                     /locus_tag="N7A07_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU2476793.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            15053..16273
                     /gene="sufS"
                     /locus_tag="N7A07_002134"
     CDS             15053..16273
                     /gene="sufS"
                     /locus_tag="N7A07_002134"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU2476794.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16286..16702
                     /gene="sufE"
                     /locus_tag="N7A07_002135"
     CDS             16286..16702
                     /gene="sufE"
                     /locus_tag="N7A07_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU2476795.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16851..17855
                     /gene="ldtE"
                     /locus_tag="N7A07_002136"
     CDS             16851..17855
                     /gene="ldtE"
                     /locus_tag="N7A07_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU2476796.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17918..18154)
                     /gene="lpp"
                     /locus_tag="N7A07_002137"
     CDS             complement(17918..18154)
                     /gene="lpp"
                     /locus_tag="N7A07_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU2476797.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18465..19877)
                     /gene="pykF"
                     /locus_tag="N7A07_002138"
     CDS             complement(18465..19877)
                     /gene="pykF"
                     /locus_tag="N7A07_002138"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU2476798.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19885..20079)
                     /locus_tag="N7A07_002139"
     CDS             complement(19885..20079)
                     /locus_tag="N7A07_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476799.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5252 a         4858 c         5148 g         4881 t
ORIGIN      
        1 tcaggtgtga ttttccctac gccagatgga taattagcgc acagagactc acgaaatatt
       61 tttattaaat acggttgaat atattttttt agttcggcgg gggaggatat tcccgccgaa
      121 atattattgc ttatgcctga tgtaaaatag ttaatactgt gcgcaaatca tttaccgaga
      181 tttgccctgg cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt
      241 caccagccag acgagaaatt acgccagttt ttgccattga catcgtgatg attggacgat
      301 cggcatactg ctcctgcatc tccagggtcg cggcaagcaa cgccagcaca tcgctggtac
      361 tttgcggcat cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac
      421 gggcaatgat ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt
      481 tcacatcatg cgcgtgggcg taggcgacgg tttctttaac ctgatcatcg ccggtaaata
      541 actccagatc gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat
      601 aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca
      661 gcggtttttc tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat
      721 tggagaggtc ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat
      781 aggcgagagc ttcggatttc actcgggcga tatctttcgc catcagcgag acgatgattt
      841 taggtgcgcc cgtaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt
      901 tctgcacgcg gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag
      961 ggaaatcttt gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt
     1021 atccatcaat cgttttgcaa ccagcttgtt gcgcctgctg caataacttc gtcatatgcg
     1081 ggttatacac gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca
     1141 atgatttatt ctcaaggggt ttcataccca cttttgtgcc attggttaaa atgtcggcgg
     1201 aagccagggc ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg acgacacaat
     1261 cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac ttatcccgac
     1321 ggttaaagag tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg
     1381 aggcacctcc ggcccctaac agcaccattg ttttgccttt gatatcaaaa ccgctctctt
     1441 taatggcgcg aatatggccc gtgccgtcgg tgttatagcc gcgcaggtag ccatcatcat
     1501 taacgatggt gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt
     1561 cacacgccag ttgtttgttc ggcattgata caccagttcc gcgcattttt agagctttta
     1621 atccttcaat tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg
     1681 gcaatcccgc tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag
     1741 gataagccat caacccaatc aattcgtatt ttgcggtaac atccataatt aactccttta
     1801 acctctatgc ttaattgtgt ttaaccgggt gctaaatttg cgctcgccaa accgcacatc
     1861 attctcagga ataataaaaa cgcggtaata gcggataaaa acaataattg cggtaatcag
     1921 ggccagcagc gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag
     1981 ataaccggta atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact
     2041 ggtgactttg gctttgcttt ttgggaaaaa ctctgacatg accgaaacac cgagctgtaa
     2101 aatgccgcca gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga
     2161 agggtacagg tagataatgg ctgctgttat tgttgccagt gcagagttaa atacattagc
     2221 ccagatgggc cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccagcga
     2281 gcccatactg taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc
     2341 catcgcatat ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc
     2401 gaacagtacc gatgaaacac cttccagcca gactaacggt ttgctgttca tttgcggtaa
     2461 ttcattagct acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag
     2521 cgtgatcagt acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt
     2581 gagcaacata tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt
     2641 aaccagtatg accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc
     2701 acccgtatcc agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt
     2761 taaattcggg caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa
     2821 tatcaccgcc cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa
     2881 tcgccccaaa ccgataccgg aaattaagta ggcaatgccc gcgttgtcag tggaaaactt
     2941 ttccgccaga gatgacatat tttgggcaag cgtaataaca ctaatgccgt gcaggaagta
     3001 gctgaagtaa atacaaagaa cagccaggat aaatggcgtg ctgaaagcct tattttgaga
     3061 cattttcaca gctccttgct gaaagataat ttcgttaccg tacgtattgt tctccgacgg
     3121 tttacataat tactatactg aagatgattt tccctgggta catggcataa agtttatgtt
     3181 tccaggaata ttgcatgtca tagtttgttt ttgatcgcga ttttccttag ttagcgcaga
     3241 aagagttatt tatccgtaag atcttgcatg acctgaaaag aatattgcat tacccacccg
     3301 gagcgacatt ttcctttagc gagtgatgcc gcgttttttt gcggctgcgt aagccgataa
     3361 acagtgccag taaaaaaccg atggcagcga tggcggtatc gaaccacata atatcggcaa
     3421 tacttctttg ggacagatga gccgtaatca acggaatagt aaaggtcgca atactgcccg
     3481 cactgtaata gatccctgta gctttacctt tagcgtaagg gaaacgttca gccattaacg
     3541 tcaggccaat ttgcacaaca cctccagcag aagtaaaacc aatgacaaaa gaaaatatta
     3601 tcaccacata aaatgtggga tgcaggcaga cggtaaacag agcaataaat gagataaagg
     3661 tgtacagcat cagtaatgtt gttgggcgaa cggtattacg aatgagtgga gcggtaataa
     3721 atacacaaag cagcgaaccc acggtgtaga tactgagtag tttgatcgac atagtgtatg
     3781 acatgcctgc aacaaattgt ccgtactgtg ccagccactg gctaaccaga taaaacgttg
     3841 ccattgagat atagccatat aaggtataac tggctaaatc gataattgaa cagcgatgtt
     3901 ccgtagagct ggtggttttc tttatgacag gtaagcgacg acccggatgg ggtgggaacg
     3961 tacaacggta taaaaacaga gcgttaataa acataatgcc tgcagcaatc ataaaggacc
     4021 aaccgaacca cagttcagcc cacactaaca ggctaatgat tagcggtaat aaaaattgtc
     4081 cgctggaaac aaatgcttta attaaaatat tggctgtccc aggtgagcgt ggaaaagctt
     4141 ccatcaaact gggataagtg cctgcatcga gaaaactgtt tgccattccc gccagaaagc
     4201 caaaaacata agcgataatg atgttattgg tctgcaggat gccaaaaaag aaggccatat
     4261 agcagcacat cccgagcatg ataaaagggc ggcgaccaaa gcgatcggat aataatcctg
     4321 caaaaagcaa gacacttaat cgaccaatgc ccagcgatga gataactatc gagacacccg
     4381 cggcattaga ctgccaaagt gtctccagcg aggccatatt caggctcatc aaaatgacgc
     4441 ccataccatg caccaggtaa ttaaaataca acccaagtgc ggtagggtag tagggatttt
     4501 tcataggttc tacttagcaa aggtgcttta aaaagtgtag cgatgttgtg tcagtatccg
     4561 ttcattattg ttgtaacaac gacaattcct ttttgtggag aaattaacaa agccgctcca
     4621 gatcgtgtgc atataattca gcggctaccg attgatagat cttccagtca ataaaattgc
     4681 catcagggta gacttgttga aacgcggtta agtcggggaa aaaacgcatg gtgttgttgc
     4741 cgatgcggtt atttattttc tctatgatcg catgaagatg tttttttctt tgctgacgca
     4801 tagccaacag ttgctcgact acacaatcag gaatggcaca cctgccattt tcccattgac
     4861 gccagctttc actgtcaccg gtctgggcaa tccaggtcgc gcattcatca atagtcatgg
     4921 caaaaatatg gcggagtgcc tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa
     4981 taaacaataa aatataaata acattatcag cctgctgacg gcttgaagag agtcacagta
     5041 tcttgtgcaa cattattgga aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa
     5101 aaaaagccca tcgtgggaga tgggcaaaga ctacacacag caattcgttg tttcactcag
     5161 gggatttcca tgcttataaa tcaatatgtt gatttataac cgtgagctaa tagtaggcat
     5221 acggactttt tgcgtcgatc agattcgtct cattagttta aaaactgtaa agaatttgcg
     5281 acaggtacta ccagctgaat ggcggtaagc aggaaaatat ggggaagagc aagcaaatat
     5341 caattagcgg tggttaacaa tcaggcggca gagccgcctg atgagaaatt acttattcgg
     5401 tttctggatt tcgccaagtt cttcgagaat ttcttccggt tgatccgtcg gcggtgggac
     5461 ctcttccacc cacgcggcaa acagacgcca ggaaacggct aacagaaccg gaccaataaa
     5521 cagaccgatc atcccgaaag caatcaaacc accaataacg ccagagagga tcaggatcag
     5581 cggtaaatcg gcacccatgc gaattaacat tgggcggatg acgttatcca gcgtgccaac
     5641 cacaccgctc cacactaaca ataccgttcc ccaggtggta tcgccagtcc agtagagcca
     5701 gataatcgcc ggaatcagta ccggcaacgg gccaagctgg acaaggcagg aaaggatcat
     5761 taacaccgtt agcaaagttg cataaggtac gccggatacg gcgaggccga taccgccaag
     5821 cactgcctgt actaacgccg tcaccaccac acccagcgcc accgcgcgga tagcctgtgc
     5881 cgccagcagt acggcggcat ctccgcgaac gcctgccaga cgggtcgcaa aatggcgaat
     5941 gccttgtgcc acctgttcac cgcgccagta cagcagggca ctgaagagaa gcatcagcgc
     6001 acaatgcacc ataaagcgcc cgatatgcgc cgcttgccca acgaaccagg tagtggtggt
     6061 gccaatataa gggcggactt tcgccatgat cgccgtcccc cccatatcca gcaagttgtg
     6121 ccagcctgca tacagcttcg cgccaatcac cggaatggta ttaagccacg ccagatcggg
     6181 taacgtcatg tcaccgctgg aaatggcttt aattagcggg ccgctgccgt cgacgatact
     6241 gttaaccagc aaagcaatag ggatgataaa caccatcact aataacagcg tcatcaccag
     6301 aacggcgaga gagcggcggc caaacatgat cttttgcaaa cgtaacaata ccggccaggt
     6361 ggcgataacc accgtaccgg cccatgcaaa gccgagaata aagggttgaa caatccacag
     6421 acatgccaca atcatgatgg ctaaaaacag caccgaaagc agaatttgtg cgacatccct
     6481 gggctgacga acatttacca taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg
     6541 cgacgtgaac acgcgaaact cacctgcata atgatgagca atttcagcgg tttttaacag
     6601 gccgattctg cccttaattc tgatgggcgt gttagaaaaa aatgtggtac aacaactcag
     6661 gcaacacgca aacgatttac tcgtcgtatt tcaagttgca tatgctgcgg ctacgttcgt
     6721 tcaccccagt tacttactgt tgtaagctcc tggagttcac tcgcttgccg ccttcctgca
     6781 actcgaaatc catagagtaa ataaacatca cctttctgtg cgctattgca gccgcctgaa
     6841 agaacagtgt atcgacctac atcacagacc gcaggaaagg gtcaatataa tgattccaca
     6901 gatttcccag gcacccggcg tcgttcaact ggtgcttaat tttttgcaag agctggagca
     6961 acaaggtttt accggcgata cggcgacaag ttatgctgat cgtctgacaa tgtcgaccga
     7021 caacagtatt taccaacttc tccccgatgc agtggtattt ccgcgttcaa ccgcagatgt
     7081 ggcgctgatc gcccgtcttg ccgcgcagga acgctattca tcgctgatct ttaccccccg
     7141 cggcggcggc accggcacta acggtcaggc gctcaaccag ggtattattg ttgatatgtc
     7201 ccgccatatg aaccgcatca tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc
     7261 cggggtgata aaagaccaac tcaatcagta cctgaaaccg ttcggctact tttttgcacc
     7321 agaactttcg accagcaacc gggcaacgct cggcgggatg atcaataccg atgcatccgg
     7381 tcagggatcg ctggtctatg gcaaaacgtc agatcacgta cttggcctac gtgcggtatt
     7441 gttggggggc gatattctcg atacgcaacc tttacccgtc gagctggcgg aaacgctggg
     7501 taaatccaat accacaatcg ggcgaattta taacacggtt tatcaacgtt gccgtcagca
     7561 acgccagtta atcatcgaca acttccccaa acttaaccgc tttcttaccg gttacgatct
     7621 gcgtcatgtc tttaacgatg agatgaccga gttcgatctg acgcgcattc tgacgggttc
     7681 agaagggacg ctggccttta ttaccgaagc gcggctggat attacgcgct tgcctaaagt
     7741 gcgccgtctg gtgaatgtca aatatgactc ttttgactct gcgctgcgta acgcgccgtt
     7801 tatggttgag gcgcgggcgc tttcggtaga gacggtggac tcaaaagtgc tgaatctggc
     7861 gcgggaagat attgtctggc attccgtcag cgagttgatt accgatgtgc ctgacaaaga
     7921 gatgctcggg ctgaacattg tggaatttgc tggtgatgat gaggcgctga ttgacgagcg
     7981 ggtaaatgca ctctgtgcgc ggttggatga gctgatcgcc agtcatcaag caggtgtcat
     8041 cggctggcag atgtgccgtg agctggcggg ggttgaacgt atctatgcaa tgcgcaaaaa
     8101 agccgttggt ctgcttggca atgccaaagg tgccgctaag ccaattccgt ttgctgagga
     8161 tacctgcgta ccgccggaac atctggccga ttatattgct gaatttcgcg cgctgctcga
     8221 cagccacggc ttaagctacg gtatgttcgg tcacgtcgac gcaggtgtct tgcatgtccg
     8281 tccggcactg gatatgtgcg atccgcaaca agagattttg atgaagcaaa tctctgatga
     8341 cgtggtggcg ctgactgcga aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt
     8401 tcgcgctgaa tacagcccgg cgtttttcgg tgaggaactt tttgcagaac tgcgcaaagt
     8461 gaaagcggca tttgacccgc ataaccgact caacccaggg aagatttgcc cgccagaagg
     8521 tctcgatgcg ccgatgatga aagtggacgc ggtgaagcgc ggtacattcg atcggcagat
     8581 ccccattgcg gtacgccagc agtggcgcgg tgcgatggag tgtaacggca acggtttatg
     8641 cttcaacttt gatgcccgta gtccgatgtg tccgtcgatg aagatcaccc agaaccggat
     8701 tcattcaccg aaagggcgcg caacgctggt gcgtgaatgg ctgcgtttgt tggcggatcg
     8761 cggcgttgat ccactcaaac tggaacaaga actgcctgaa tccggcgtca gcttgcggac
     8821 gttaattgcc cgcacgcgca atagctggca tgcgaataaa ggcgaatatg acttctcaca
     8881 cgaagtcaaa gaggcgatgt cgggctgtct ggcctgtaaa gcttgttcga cccagtgccc
     8941 catcaaaatt gatgtgccgg agtttcgctc tcgttttctg cagctctatc acacccgtta
     9001 tttacgcccg ctgcgcgacc acctcgtcgc tacggtcgag agctacgcgc cgctgatggc
     9061 acgcgcgccg aaaaccttta acttcttcat taaccagccg ctggtgcgca aactctcgga
     9121 aaaacatatc ggcatggttg atttgccgct gctgtcggtc ccctcgctac aacaacaaat
     9181 ggtggggcat cgctcggcaa acatgacgct ggaacagctt gaatctctca atgcagagca
     9241 gaaagcgcgc acagtgttgg tggtgcagga cccctttacc agctattacg atgcgcaagt
     9301 ggtggcggat tttgtccgtc tggtcgaaaa attaggtttc cagcctgtgt tactgccatt
     9361 ttcgccaaat ggcaaagccc agcatattaa aggttttctt aatcgttttg cgaagacggc
     9421 gaaaaagacg gcggatttcc tcaaccgtat ggcgaagctg ggtatgccaa tggtgggcgt
     9481 cgatccggcg ctggtacttt gttatcgcga tgaatataaa ctggcgctgg gcgaggagcg
     9541 tggcgagttt aacgtcttac tggcgaatga atggctggca agcgcacttg agtcacagcc
     9601 agtggctaca gtcagcggtg aatcatggta tttctttggt cactgtaccg aagttaccgc
     9661 cttgccgggt gcgccagcac aatgggccgc gatatttgcc cgttttggcg cgaaactgga
     9721 aaatgtcagc gtgggttgct gcggcatggc agggacttac ggacatgaag cgaaaaacca
     9781 taaaaattcg ctcgggatct atgagttatc ctggcatcag gctatgcagc gactgccgcg
     9841 taaccgctgt ctggcgaccg gatattcctg ccgtagtcag gtcaaacggg ttgaaggcac
     9901 gggggtacgc catcctgtgc aggctttact ggagattatt aaatgatatg gaaacgaaaa
     9961 atccccctgg aagcactgaa tgccatgggt gaaggaaaca tggtggggtt actggatatt
    10021 cgctttgaac atattggtga tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca
    10081 aagcagcctt tcgggttgct acatggaggt gcatccgtgg tactggccga aagtatcggt
    10141 tccgttgccg gttatttatg taccgaaggt gagcaaaaag tggttggtct ggaaatcaat
    10201 gctaaccacg tccgctcggc acgagaaggg cgggtgcgcg gcgtatgcaa accgttgcat
    10261 ctcggttcgc gtcaccaggt ctggcagatt gaaatctccg atgagaaagg gcgtttgtgc
    10321 tgttcgtcac gattgacgac cgccattttg tgacgttgtc accagaaaag tgtgacgcat
    10381 attgcttttg gtcattatct gcacagtaaa cggtggtata ctgtgcaggt cttgtctaaa
    10441 agcgaggatg ctatgtctac tcaacttgat cccacccagc tggccattga atttttacgc
    10501 cgtgaccaga gcaatctttc acctgcccaa tatcttaaac gtctgaaaca actagaactt
    10561 gaatttgctg acctgcttac tctctcttct gctgaattaa aagaagaaat ctactttgcc
    10621 tggcgtctgg gcgtgcattg atcctgactt aatgccgcaa gttcgccaaa tcgacctgga
    10681 ttaacctccg tccaggttcg atgaccttct taatactgct tatctacccg cctgatattg
    10741 ttcaaaaata atactaatcg cagtttgtgt taaaacggcg gcttagcttt atgagattat
    10801 tcttatcgcc ccttcaagag ctaagccact gagagtgccg gagataagcg ccggatgggg
    10861 tagaaaccct taagcctgtg tcgcacagac ttaagggttt tcttatttct gaacgctgca
    10921 cttttttacc tatctcttaa atagtcattt tcaatacaac tttttctttt tcatcacagc
    10981 cttaatgctc cttagataat aatcattatc taacaatgag atacctaatt cttagcgtgc
    11041 ctgttaaccc acacatcagg gtctatgctt attaaaagca ggcaaaaagc atgttttacg
    11101 gtaaagcccc tgcctttgct gggttgaact gataatcatt atcactaaca tgctgttata
    11161 cgctgaaagc gatgaagtga ggtaaatcga tggacatgca ttcaggaacc tttaatccac
    11221 aggatttcgc ctggcaaggc ttaacgctga cacccgcagc cgctgtccac atccgcgagc
    11281 tggtggcaaa gcagccgggt atggtcggcg tgcgcttagg tgtgaagcaa acgggctgcg
    11341 cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat ctgctgtttg
    11401 aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat gccgtttatt gatggcacgg
    11461 aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac cctaaagccc
    11521 agaatgaatg tggctgtggc gaaagctttg gggtataggc ggtactatgt ctcgtaatac
    11581 tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata aagaaggatt
    11641 cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg tggtgcgcgc
    11701 aatttcggcg aagcgtaatg agccggagtg gatgctggag tttcgtctaa acgcctatcg
    11761 cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca agctgaatta
    11821 tcaggattac agctactact cggcaccatc gtgcggtaat tgtgacgaca cttgcgcgtc
    11881 tgaacctggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag aggtggaggc
    11941 ggcgtttgag cagttgggtg ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat
    12001 tttcgactca gtttcggttg ccactactta tcgcgaaaaa ctggcggagc agggaattat
    12061 tttctgttcc tttggtgagg cgatccacga tcacccggaa ctggtgcgta aatatctcgg
    12121 caccgtggtg ccggggaatg acaacttctt tgccgcgctt aatgcggcgg tagtctctga
    12181 cggtacgttt atttatgtgc ctaaaggcgt gcgctgcccg atggaacttt ccacctattt
    12241 tcgcattaac gcggaaaaaa ccggacagtt tgagcgcacc attctggtgg ccgacgaaga
    12301 cagctacgtc agctacattg aaggctgttc cgctccggtg cgtgacagct atcagttgca
    12361 cgcggcagtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt ccacggtaca
    12421 aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca ccaagcgtgc
    12481 tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg ggtcagcgat
    12541 tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg agttttactc
    12601 agtggcgctg accagcggtc atcagcaagc ggataccggc accaagatga tccacatcgg
    12661 taaaaacacc aaatcgacca ttatctcgaa agggatctct gccggacata gccagaacag
    12721 ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg
    12781 cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg
    12841 caataacagc gcacaactgg agcacgaggc aacgacatca cgtattggtg aagatcaact
    12901 gttttactgc ctgcaacgcg ggatcagcga agaagacgcc atctcgatga ttgttaacgg
    12961 tttctgcaaa gacgtgttct cagaactgcc gctggaattt gccgttgaag cacaaaaact
    13021 cctcgccatc agtcttgaac acagcgtcgg ataaggaata aacatgttaa gtattaaaga
    13081 tttacacgtc agcgtggaag ataaagctat cctgcgtgga ttaagcctcg acgttcgtcc
    13141 cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct tatcggcaac
    13201 gcttgccggg cgagaagatt atgaagtgac gggcggcacg gttgagttca aaggcaaaga
    13261 tttgcttgca ctgtcgccgg aagatcgtgc aggcgaaggc atctttatgg ccttccagta
    13321 tccggtggag attccaggtg tcagtaacca gtttttcctg caaacggcac ttaatgcggt
    13381 gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt tgatggaaga
    13441 gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa acgttggttt
    13501 ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg gcggtgctgg aaccggagtt
    13561 atgcattctt gatgaatcgg actccgggct ggatatcgac gcattaaaag tggtcgccga
    13621 tggcgtgaac tcgctgcgtg atggcaagcg ctcattcatc attgttacgc actaccaacg
    13681 cattctcgac tacatcaagc ctgattacgt tcatgtgcta tatcagggac gaattgtgaa
    13741 atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct ggcttaccga
    13801 acagcagtaa cgcgctgcaa cagtggcatc acttgtttga agctgaaggg acaaaacgct
    13861 ccccgcaagc acagcagcat ttacaacaat tgctgcgtac cggactgccg acacgtaaac
    13921 atgaaaactg gaaatatacg ccgctggaag ggctgaccaa tagtcagttt gtcagcattg
    13981 cgggagagat atccccacag cagcgagatg ccttagcgtt aacgttagac gctgtgcggc
    14041 tggtatttgt cgatggacgt tacgtgcctg cgctgagcga tgcgactgaa ggcagcgggt
    14101 atgaagtgag cattaacgac gaccgtcagg ggttacccga tgctattcag gcggaagtgt
    14161 ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgtg aagcgcggtc
    14221 aacggccggc aaagccattg ctgttaatgc atatcaccca gggcgtggca ggtgaagagg
    14281 tgaacactgc ccattaccga catcatctgg agctggcgga aggtgccgaa gcaacggtga
    14341 tcgaacattt tgtcagcctt aatgatgctc gtcattttac cggggcacgg ttctctatca
    14401 acgtcgcagc gaatgcccac ttgcagcata tcaagctggc gtttgaaaac ccgctcagtc
    14461 accactttgc ccataacgat ttgttgctgg ctgacgatgc caccgcattt agccacagtt
    14521 tcctgctggg tggcgcagtg ttacgacaca acaccagtac gcaactcaat ggcgaaaact
    14581 gcacgctgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt gatacccgta
    14641 cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa actatcgtca
    14701 gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt cgcgcagcac gccatcaaaa
    14761 cggatggtca gatgaccaac aataatctgc tgatgggcaa actggcggaa gtggatacga
    14821 aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg acggtggggc
    14881 gtattgatga tgaacagatg ttctatctgc gctcgcgcgg gatcaatcag caggatgccc
    14941 agcagatgat catttacgcc tttgctgccg aactgacgga agcactgcgt gatgaggggc
    15001 ttaaacagca ggtgctgacc cgaatcggtc aacggctgcc aggaggtgca agatgacttt
    15061 ttccgtcgac aaagtgcggg ccgactttcc ggtgctttcg cgtgaggtaa acggtttgcc
    15121 gctggcttat ctcgacagcg ccgccagtgc gcagaaaccg agccaggtga ttgacgccga
    15181 ggccgagttt tatcgtcatg gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc
    15241 ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta ttaatgcccg
    15301 ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa
    15361 tagctggggc aacagcaacg tgcgggcggg cgataacatc atcataagtc agatggagca
    15421 ccacgctaac attgttccct ggcagatgct ttgcgcacgc gttggcgcag agctgcgtgt
    15481 gatcccgctc aatcccgacg gtacgttgca actggagacg ctgcctacgc tgtttgatga
    15541 gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg cttggcacag aaaatccact
    15601 ggcggaaatg atcacgcttg cgcaccagca tggcgcaaaa gtgctggtgg atggcgctca
    15661 ggcggtgatg catcatccgg tggatgttca ggcgctggat tgcgattttt acgtgttttc
    15721 cgggcataaa ctgtatggcc ccaccgggat tggcattctt tatgtcaaag aagccttgct
    15781 acaggagatg ccgccgtggg aagggggcgg ttctatgatc gccaccgtca gcctgagtga
    15841 aggcactacc tggaccaaag caccgtggcg gtttgaagcc ggtacaccca ataccggggg
    15901 catcattggt cttggcgcgg cgctggagta tgtttcggcg caggggctta ataacatagc
    15961 cgagtatgaa cagaatctga tgcattacgc gctatcacag ctggaatctg taccggatct
    16021 cactctctat ggcccacaaa acaggcttgg cgttattgct tttaatctcg gtaaacacca
    16081 cgcctatgat gttggcagtt ttctcgataa ttacggcatt gctgtgcgta ccggacatca
    16141 ctgcgctatg ccattaatgg cctattataa cgtccctgct atgtgtcggg cgtcgctggc
    16201 catgtataac acccatgaag aagtggatcg tctggtgacc ggactgcaac gtattcaccg
    16261 tctgctggga taacagggag gcactatggc tttattgccg gataaagaaa agttgctgcg
    16321 taatttttta cgctgcgcca actgggaaga gaaatatctc tacattattg agctgggcca
    16381 gcgtctgcca gaattacgcg acgaagacag aagcccacaa aatagcattc agggctgcca
    16441 gagtcaggtg tggattgtca tgcgtcagaa tgcgcaggga attattgaat tgcagggcga
    16501 cagcgatgcg gcgattgtga aagggcttat tgcggtcgtc tttattctct acgatcagat
    16561 gacgccgcag gatgttgtca atttcgatgt gcgtccgtgg tttgaaaaaa tggcactcac
    16621 ccaacatctc accccatctc gttcacaagg tctggaagcg atgattcgcg caattcgcgc
    16681 caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg ctcacattgc
    16741 cggatggtca atgtttacac tgacctgcac aggagtgttt cggtttcagg tgaacataag
    16801 cgccatccga cgtttcagcg tgagtctccg gcaaatacag gaggtttaca atgaaacgcg
    16861 cgtctttgct tacactcacg cttatcggcg cttttagtgc catccaggct gcctgggcgg
    16921 ttgattatcc gctaccacca accggaagcc gactggttgg gcaaaatcaa acgtatacgg
    16981 tgcaggaagg ggataaaaac cttcaggcca tcgcccgacg ttttgatact gcggcaatgt
    17041 tgatccttga agccaataac actatcgccc cggtgccaaa acccggtacg acgataacta
    17101 ttccttcgca actgttatta cctgatgcgc cgcgtcaggg gattatcgtt aaccttgcag
    17161 agctgcgcct ttattattat ccgccgggag aaaatatcgt gcaggtttat ccgataggta
    17221 ttggcttgca ggggctggaa acgccagtga tggaaacgcg tgtggggcag aaaatcccta
    17281 acccaacctg gacgcctacg gcaggcattc gtcaacgttc gctggagcgt ggcattaaat
    17341 taccgccagt cgttcctgcc ggaccaaata acccgctagg acgttacgca ctgcgcctcg
    17401 cgcatggtaa tggcgaatac ctcattcatg gcaccagtgc gccggacagc gtcggtttgc
    17461 gcgtcagttc agggtgtatt cgcatgaatg caccggatat taaagccttg ttctccagcg
    17521 tgcggacggg aacgccggtg aaagtgatca acgaaccagt gaaatattcc gtggagccta
    17581 acgggatgcg ttatgttgaa gtacatcgac cactatcggc agaagaacag cagaacgttc
    17641 agacaatgcc atacacactg ccagcaggct ttacgcaatt taaagacaat aaggctgtag
    17701 atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg gtatccggtt gcggtgagca
    17761 gtggagcaac tcccacagcc agcaatgcgc cttcagtaga gtcagcgcag aatggtgaac
    17821 cagagcaagg gaatatgtta cgcgcgacgc agtagtggta aatagcagac aaaaaaatgg
    17881 cgcacaatgt gcgccatttt tcacttcaca ggtactatta cttgcggtat ttagtagcca
    17941 tgttgtccag acgctggtta gcacgagctg cgtcatcttt agcagcctga acgtcggaac
    18001 gcattgcgtt cacgtcgttg ctcagctggt caactttagc gttcagagtc tgaacgtcag
    18061 aagacagctg atcgatttta gcgttgctgg agcaacctgc cagcagagta gaacccagga
    18121 ttaccgcgcc cagtaccagt ttagtagctt tcattattaa taccctctag attgagttaa
    18181 tctccatgta gcgttacaag tattacacaa agttttttat gttgagaata tttttttgat
    18241 gggaaggcac ttatttttga tcgttcgctc aaagaagcat cgaacgctgg gttttagata
    18301 aaaaaattga gagaaaacag gatgcttcca tcggattcat cttagataaa gtgagattat
    18361 atagtgaaat ccgatttgat tttttattgc ttctggttat cgattaaata aaaaaagcgc
    18421 ccatcagggc gcttcgatat acaaattaat tcacaaaagc aatattacag gacgtgaaca
    18481 gatgcggtgt tagtagtgcc gctcggtacc agtgcaccag aaaccataac tacaacgtca
    18541 cctttgtgtg ccagaccgct ctgcagagcc agttctttac ccagacggta gaaatcatca
    18601 gtagaagtga tctctttaac cagctgcggc acaacgcctt tgctcagtac caactgatga
    18661 gccgtttttt cgttggtggt cagtgccagg atggtggcat ccgggaagta tttacgtact
    18721 gcgcgagcag atttaccgcc ctgggtagca accacaatca gcggagcatc cagtttttca
    18781 gcagtttcaa cggcaccacg gcataccgct tcggtaatgc gcagtttacg gttgtcattg
    18841 ttgaactcga gacggctgtt catcacgcgg tcggtacgtt cgcagatggt cgccatgata
    18901 gaaaccgctt ccagcgggta tttacctttt gcggattcac cagacagcat cactgcgtca
    18961 gtaccgtcga ggatggcgtt tgcaacgtca ccggcttctg cgcgagtcgg gcgtgggttt
    19021 ttgatcatgg aatccagcat ctgggtcgca gtgataacga ctttacgtgc acggatacat
    19081 ttttcgatca tcatcttctg ggcgaagata acttcttcta ccgggatttc tacacccagg
    19141 tcgccacgcg caaccatgat gccgtcagag gcttcgagga tttcgtcgaa gttgttgagg
    19201 ccttcctggt tttcgatttt ggagatgatg tggatgtttt cgccgccgtg cgctttcagg
    19261 tgctcacgga tttcgataac gtcagaacgc ttacgaataa aggaagcagc gacaaagtct
    19321 acgccttgtt cgcaaccaaa gatcaggtcc tgtttgtctt tttcagccag tgctggcagg
    19381 gcaatggaaa cgccaggcag gttcacacct ttgttttcac ccaggtcgcc gttgttcagc
    19441 actttacaga taactttgtt accttcaatg gcagtaactt ccataccgat cagaccatcg
    19501 tcaaccagta cggtgttgcc aacagacagg tcagttgtga aaccttcata cgttaccgca
    19561 accatttcgc tgttgccgat aacagattta tcagtagtga aagtaaaggt ctgaccagct
    19621 ttcagagaaa cgtcgttacc gccttccagt ttcatggtgc ggatttccgg acctttggta
    19681 tcaagcagga tagcggcggt tttaccagtt ttgctcatca cgttgcgcag attctgaatg
    19741 cgctgaccgt gttctgcata gtcaccatga gagaagttca gacgcataac gttcatgcca
    19801 gcgtccagca ttttagctaa catctcttca gattcggttt tcggtccgat ggtgcaaaca
    19861 attttggtct ttttcatgac agtcttagtc tttaagttga gaaggatggg agaaacttgc
    19921 tttctgggcg cactgccgga aagcgaatcc ggtattacga aagtggtggt gcctcgctct
    19981 aaggataggt gacatcgaaa aagcgtgcag aggaatgtgt gcttgtgtct cagcccaacg
    20041 tgagtgcctg tttttgagtc gttgagttct acagtccaaa gtgctgaaac cattcaagag
    20101 tcaattggcg cgcattatac gctgaaatta atccaaaag
//
LOCUS       DBFIFS010000064        19846 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.064, whole genome shotgun sequence.
ACCESSION   DBFIFS010000064 DBFIFS010000000
VERSION     DBFIFS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.064"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..616
                     /gene="ompL"
                     /locus_tag="N7A07_002140"
     CDS             <1..616
                     /gene="ompL"
                     /locus_tag="N7A07_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU2476800.1"
                     /translation="REAYNLASDQGEVMLRVGYNFDMGAGIMLTNTYTFQREDELKHG
                     YNEIEGWYPLFKPTDKLTIQPGGLINDKSIGSGGAVYLDVNYKFTPWFNLTVRNRFNH
                     NNYSSTDLNGDLDNNDSYEIGNYWNFIITDKFSYTFEPHYFYNINDFNSGNGTKHHWE
                     ITNTFRYRINEHWLPYIELRWLDRNVEPYHREQNQIRIGTKYFF"
     gene            complement(707..1972)
                     /locus_tag="N7A07_002141"
     CDS             complement(707..1972)
                     /locus_tag="N7A07_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2476801.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(2074..3054)
                     /locus_tag="N7A07_002142"
     CDS             complement(2074..3054)
                     /locus_tag="N7A07_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU2476802.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(3062..3772)
                     /locus_tag="N7A07_002143"
     CDS             complement(3062..3772)
                     /locus_tag="N7A07_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2476803.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3989..5812)
                     /gene="typA"
                     /locus_tag="N7A07_002144"
     CDS             complement(3989..5812)
                     /gene="typA"
                     /locus_tag="N7A07_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU2476804.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            6185..7594
                     /gene="glnA"
                     /locus_tag="N7A07_002145"
     CDS             6185..7594
                     /gene="glnA"
                     /locus_tag="N7A07_002145"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU2476805.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            7768..8817
                     /gene="glnL"
                     /locus_tag="N7A07_002146"
     CDS             7768..8817
                     /gene="glnL"
                     /locus_tag="N7A07_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU2476806.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            8829..10238
                     /gene="glnG"
                     /locus_tag="N7A07_002147"
     CDS             8829..10238
                     /gene="glnG"
                     /locus_tag="N7A07_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU2476807.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            10350..10460
                     /locus_tag="N7A07_002148"
     CDS             10350..10460
                     /locus_tag="N7A07_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU2476808.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(10650..12023)
                     /gene="hemN"
                     /locus_tag="N7A07_002149"
     CDS             complement(10650..12023)
                     /gene="hemN"
                     /locus_tag="N7A07_002149"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU2476809.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(12212..12718)
                     /gene="yihI"
                     /locus_tag="N7A07_002150"
     CDS             complement(12212..12718)
                     /gene="yihI"
                     /locus_tag="N7A07_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU2476810.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(12837..13003)
                     /locus_tag="N7A07_002151"
                     /pseudo
     CDS             complement(12837..13003)
                     /locus_tag="N7A07_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13300..13932
                     /gene="yihA"
                     /locus_tag="N7A07_002152"
     CDS             13300..13932
                     /gene="yihA"
                     /locus_tag="N7A07_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU2476811.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(14314..17100)
                     /gene="polA"
                     /locus_tag="N7A07_002153"
     CDS             complement(14314..17100)
                     /gene="polA"
                     /locus_tag="N7A07_002153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU2476812.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            17464..18396
                     /locus_tag="N7A07_002154"
     CDS             17464..18396
                     /locus_tag="N7A07_002154"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU2476813.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(18437..>19846)
                     /locus_tag="N7A07_002155"
     CDS             complement(18437..>19846)
                     /locus_tag="N7A07_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HGU2476814.1"
                     /translation="GVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKKKGEHAYD
                     MTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGN
                     VIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFS
                     FGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVG
                     ESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLG
                     SGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPL
                     LQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVT
                     ETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVF
                     NGQEMSEEEFMSRAGRFVH"
BASE COUNT         4860 a         5001 c         4756 g         5229 t
ORIGIN      
        1 cagggaggca tataatctcg cctccgatca gggcgaggtt atgctacgtg tgggttataa
       61 cttcgatatg ggcgcgggta ttatgttaac caatacctac acctttcagc gagaagatga
      121 actaaaacat ggatataacg aaatcgaagg ttggtatccg ttatttaaac caaccgataa
      181 attaaccatc cagcccggtg gcttaattaa tgataagagt atcggttcgg gtggtgctgt
      241 ttatctggat gttaactaca aatttactcc gtggtttaac ctgacagtgc gcaaccgttt
      301 taaccataat aactacagct caaccgattt aaacggtgat ctggataata atgacagcta
      361 cgagattggc aactactgga actttattat taccgataag ttctcatata cctttgagcc
      421 gcattatttt tataacatta atgatttcaa tagcggcaat ggcacgaagc atcactggga
      481 aatcactaac accttccgtt accgcattaa cgagcactgg ctgccctata ttgaattgcg
      541 ttggttagat cgcaacgtgg aaccgtacca ccgcgagcaa aaccagattc gtatcgggac
      601 gaagtatttc ttctgatgtg aaactgccgg atgcggcgta aacgccttat ccggcctacg
      661 gttgatacgg ttttcgttgg ccggattaag tacatccggc cattaattaa gcttcagccg
      721 ccagcatctc tttcttacgt tggctaatac gcttaaccag cattacggaa accaccgcac
      781 cacagaatgc gaagcaggcc ataatgccaa acacgatttt gtagccaata atccccggat
      841 ttaaatccag aatgtagcca tacagactga agcagaacat cgccggggcg taaccaataa
      901 agctacccaa cgccatcgcc gcgcctgttt tattttcagc aatttttgct tcgccgatag
      961 gtgcaaaaaa tacggctcgc tgtgtaaaga ctatcgcgcc aaagcccagc gtacatgcca
     1021 tccctaaata gaccggcata ctttcgtgcg gcagcataat caacagtacg agcgcagcgg
     1081 tactgatgat aaaggtgtag catagatatt tactcggcga tttcaggatt ttatctgaaa
     1141 tcatgccacc aatcggtcca ccaatcattt tcagacagta ttggttaatg atgccgtaag
     1201 cccccaccag cgcaacgggc aatagataga tgtttttcag gaatggaatg aagaatgtca
     1261 ggccacagta aaccgcgtaa acgaagaaga cgttgaaagc gataagccag atagttttgt
     1321 ctttcagcac cgaggtcata ctggtgtttt gcgatgcccc gtcttctttt ttcacctcaa
     1381 cggacggtgc ctcttctttg tcattcagga caaagaaaat agtaatgcct acggcaatca
     1441 caataaggga atagaaccag atgcctgctt taaaacctaa taagccactg ccaaaccagg
     1501 taaatactgc caacgcagaa aatgccacca cggtatcgac aatgccacgc cctgtttcga
     1561 agaagccaaa caaccgacct tgttgttcgc tattgcccaa tcgacttacc gacttgagca
     1621 agaccggcca gttcatcatg tcgcaagtaa cgccaaatag cgcccagaca aagaggatgc
     1681 cccaataccc cggcattgtc gtcaaataaa cacccagtaa tcctgtcgca atgagtgaga
     1741 atgacatggt gtatctgcgc ggtagtttat cggcaaaata aatagacaga aaaaagccca
     1801 ctgtggtaac aaatgagttt accgacatag cattaccaat ttgaccattg gtcaaatgga
     1861 aatattcctg catcgggata taaaacgcat ctttcagcga cggtaattta taaattgtac
     1921 cgccacacag tgttaataga ctaaataacg cccatttctt tttcgtgagc ataacatccc
     1981 ctaagcgatt ttaaaaatca ctgttttaaa ggcaataaga taccgaccat tcttatggaa
     2041 tggttttatt aactgcgtat gagttaaaac ttgttagtgg ttcagaagat ggctcgcctc
     2101 ttgtttgatt tgttgtagta cgttacgcac atgatttatc tgattaattg catcttcttt
     2161 ttctttacgt aaccgctcat ccagcaaatg cttttctggc aatggtgttt cactcatctg
     2221 gcgataaggg atccacggat tatcatcttc gtcttcaaag cggaacgctg gagcataata
     2281 atcaacctct tcttccaggc catatgccgt cacctgcggt tcttcatcgc cgaggcagat
     2341 aaggtgtgtt aataactctt tgaacggcat atctccctga cctgaaatac aggctttatg
     2401 acccaggcca ccttgttctt taacgatcaa ggcatctttg atatggacct gggtaatatg
     2461 cggtgccatc gtttttaagg cgtctatggg atgctcattt gcattgatca tattcgcaaa
     2521 atcaaacagt aaggaaagag actccatctc actttctttg accagcgaca ccaactcatg
     2581 actttttaaa tcttcatgct gttcgatcgt aaaagtcagg ccgctatcct gatacgcttc
     2641 ccgcacatag gcaatgtcgt tagcgatgat cgataatacg tcgcgcaaat taccttcata
     2701 acgtggataa aaacgtacgg acgatgcccc agttttcaac gcgatggcga cggcttcgtc
     2761 aatagatgcc ttatctgagg cgctggtttc aatatgaata tcaaggttca gacggcgggc
     2821 tttatcccca aaggcagaga gttctttatc gtccatatta ccaagagaaa aacgctcgcc
     2881 atccagaaca tggattttga cgccacgtag attattttca cttgcgatat caagtaaatc
     2941 agcaggcaac actctttcca gccgcatatt taaatgaaag gcataagcat gtaaataaag
     3001 aggaagagtg tcgacacgtt gtagaatctt tcttgcgtta tttattgtca ccataaaaca
     3061 cctacagatt aatgatgagg tctacggcct caaatcgcca atattcaata tctatctgga
     3121 caatattgcc ttgctggtcg gcgcgatgct tttcaattaa gatcgctggc atacccgcag
     3181 aaccgccaag atacttgctg atatcgcccg gcattctgac gggtttgaaa atcaattttt
     3241 ttactaccgt ctctttttgg tagcactttt cccagactgc agaaaatgag tggttttcaa
     3301 gttgttcaat aaaacccgga gcaacttcag gattaatata tgtttcgtga tagaaaactt
     3361 tatgtccttc cagcgccccc cagccggtga tgcgatagag ttcagttgat ggtgtcactg
     3421 caatcagtgg cgcgagcgtc tcgggaatat cgctggtacg gtttttctcg gtaaaccccc
     3481 aggaaggctc tcgcccttgc tcaattgcgg cccgctgaaa gctcgccgac agctccggac
     3541 tgtaattaaa tcgcggctgg gtcacgaacc accccttacg atccttacgg aagattttgg
     3601 attctgcctc gaggttcaac aacgcctggc gcagcgtcat acgtttaatg cccagcagtt
     3661 ctcccagctc tctttctgaa gggagttttc cacccggcgt ggtgatgcca tctttcaacc
     3721 accgatccag tttctctttt gcattttcta cggtggattg attctccgcc attgtttaat
     3781 ccttttggtt taaaccagat gaagcatctt acgcttacgc caaaaaagaa agcaattaac
     3841 ggcgtatgac tttgcagata attgtgacaa agctctctat tgacgtaacc acagacttat
     3901 aagggagata ctcgcattgg tgtgcgctca cgacaggcaa aaaaaaacct gccatcgctg
     3961 gcaggttttt tatgactaaa aaacgaaatt aatcgtcttt cggtgcgcgg ttggcgcgac
     4021 gacgatcgtt ttccgtcagg tgacgtttac gaatacggat agaggtcgga gtcacttcta
     4081 ccagttcgtc atcatcgatg aactccagag cttgttccag agtcatgcgg ataggcggaa
     4141 ccagaacaac ggcttcgtca gtaccggaag cacgcatgtt ggtcagtttc ttaccggtca
     4201 ggcagtttac agtcaggtcg ttagagcggc tatgaatacc gataatctga ccttcgtaaa
     4261 cttctgcacc gtgaccgagg aacagcttac cgcgatcctg caaaccgaac agcgcgaacg
     4321 cgaccgcttt accctgaccg ttagagatca gtacgccgtt ctgacgctga cccacttcac
     4381 ccggacgcac gtcgtcgtag tggctgaagg tggagtacag cagacccgta ccggaagtca
     4441 tggtcatgaa ctcagaacgg aagccaatca gaccacggct tgggatcacg tagtcgagac
     4501 gtacgcggcc tttaccgtct ggattcatgt ttttcaggtc gcctttacgt tcgcccagcg
     4561 cctgcattac agaaccctga tgctgttctt caacgtccag agtcacgttt tcatacggct
     4621 cttgtttacg accgtcgatt tcacggaaga taactttcgg acgggatacc gccagttcga
     4681 aaccttcacg acgcatgttt tcgatcagaa cagacaggtg cagttcgcca cgaccagaaa
     4741 cgcggaacgc atcggcgtct tcggtttctt ctacgcgcag cgcaacgttg tgtaccagtt
     4801 ctttgttcag acgatccagg atctgacgag acgttacgaa cttaccttct ttaccgcaga
     4861 acggcgaggt gttaacgcag aagaacatag aaacggtcgg ctcatcaacg gagagtgccg
     4921 gcagcgcttc aacgttttgc gtgtcgcaaa cggtgtcaga aatgttcagt tcgcccaggc
     4981 ccgtgatcgc cacgatatcg ccagcttccg ccagatcggt ttcgatacgt tccagaccga
     5041 ggtggcccag cactttacca actttcgcgt tgcgggtttt gccttcgcta tcgatgatag
     5101 taacctgctg gttcggcttc actttaccgc gcttgatgcg gccaatgccg ataacgccaa
     5161 catagctgtt gtaatcgagc tgagaaatct gcatctggaa cggaccgtca aggtcaacgt
     5221 ccggcgcagg aacgtggtca acaatcgcct ggtacagcgg ggtcatgtct tccgccatat
     5281 cttcgtggtc cagacccgcg ataccgttca gcgcagaagc gtaaacgatc gggaagtcca
     5341 gctgctcgtc ggtcgcgtcg aggttaacga acagatcgaa tacctgatcc acaacccaat
     5401 caggacgcgc gccagggcgg tcaactttgt tgataacaac aatcggcttc aggccgtaag
     5461 caaacgcttt tttggttacg aagcgcgttt gcggcatcgg gccgtcaaat gcgtcaacca
     5521 ccagcagcac tgagtctacc atggacatta cacgttcaac ttcaccaccg aagtcggcgt
     5581 gccccggggt atcaacgatg ttgatacggt aatcattcca tttgatagcg gtgtttttcg
     5641 cgaggatggt aatcccacgc tctttctcca aatcgttgga gtccatcacg cgctcttggg
     5701 tttcggcacg agagtcgaac gtaccggatt gttggagcag cttgtctacc agggtggttt
     5761 taccatggtc tacgtgcgcg atgatggcga tattacgcaa tttttcgatc acaactttgc
     5821 ctcaggcatt agaaatagcg cgttattgta cacggattaa tcgcactaca aaacaggatc
     5881 acaaacatcc tccgcaaaca agtattgcag agtccctttg tgatcgcttt cacggagcat
     5941 aaaaagggtt atccaaaggt cattgcacca atatggtgct taatgtttcc attgaagcac
     6001 tatattggtg caacattcac atcatggtgc agcccttttg cacgatgtta agcatgataa
     6061 cgccttttag gggcaattta aaagttggca cagttttcgc tttatctttt ttacggcgac
     6121 acggccaaaa taattgcaga tttcgttacc acgacgacaa tgaccaatcc aggagagtta
     6181 aagtatgtcc gctgaacacg tactgacgat gctgaacgag cacgaagtga agtttgttga
     6241 tttgcgcttc accgatacca aaggtaaaga acagcacgtc actatccctg ctcatcaggt
     6301 gaatgctgaa ttcttcgaag aaggcaaaat gtttgacggc tcctcgattg gcggctggaa
     6361 aggcattaac gaatccgaca tggtgctgat gccagacgca tccaccgcag tgattgaccc
     6421 gttcttcgcc gactccaccc tgattatccg ttgcgacatc cttgaacctg gcaccctgca
     6481 aggctatgac cgtgacccgc gctctattgc gaagcgcgcc gaagattacc tgcgttccac
     6541 tggcattgcc gacaccgtac tgttcgggcc agaacctgaa ttcttcctgt tcgatgacat
     6601 ccgtttcgga tcatctatct ccggttccca cgttgctatc gacgatatcg aaggcgcatg
     6661 gaactcctcc acccaatacg aaggtggtaa caaaggtcac cgtccggcag tgaaaggcgg
     6721 ttacttcccg gttccgccgg tagactctgc tcaggatatt cgttctgaaa tgtgtctggt
     6781 gatggaacag atgggtctgg tggttgaagc ccatcaccac gaagtagcga ctgctggtca
     6841 gaacgaagtg gctacccgct tcaataccat gaccaaaaaa gctgacgaaa ttcagatcta
     6901 caaatatgtt gtgcacaacg tagcgcaccg cttcggtaaa accgcgacct ttatgccaaa
     6961 accgatgttc ggtgataacg gctccggtat gcactgccac atgtcgctgt ctaaaaacgg
     7021 cgttaacctg ttcgcaggcg acaaatacgc aggtctgtct gagcaggcgc tgtactacat
     7081 tggcggcgta atcaaacacg ctaaagcgat taacgccctg gcaaacccga ccaccaactc
     7141 ttataagcgt ctggtcccgg gctacgaagc tccggtaatg ctggcttact ctgcacgcaa
     7201 ccgttctgcg tctatccgta ttccggtggt ttcttctccg aaagcacgtc gtatcgaagt
     7261 acgtttcccg gatccggcgg ctaacccgta cctgtgcttt gctgccctgc tgatggccgg
     7321 tcttgatggc atcaagaaca agatccatcc gggcgaagcc atggacaaaa acctgtatga
     7381 cctgccgccg gaagaagcga aagagatccc acaggttgca ggctctctgg aagaagcact
     7441 gaacgagctg gatctggacc gcgagttcct gaaagccggt ggcgtgttca cggatgaagc
     7501 gatcgatgcg tacatcgctc tgcgtcgcga agaagatgac cgcgtgcgta tgactccgca
     7561 tccggtagag tttgagctgt actacagcgt ctaatagttg aagttgtact acccggcgca
     7621 acaacgccgg gatttagttg ccgtggaaac tttcagccca tctctgcatg ggcttttttc
     7681 tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat
     7741 ggtgcaacct gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag
     7801 atcctcaact cgctgattaa cagtattttg ttaatcgatg acaacctggc gatccattac
     7861 gccaaccccg ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacgccg
     7921 ttaccggaac tgttgagcta cttctcatta aatattgagc tgatgcaaga aagtctggag
     7981 gcggggcaag gttttaccga taacgaagtg acgctggtca tcgacggtcg ctcgcatatc
     8041 ctttctgtga cggcccagcg tatgccggac ggcatgatcc tgctggagat ggcgccgatg
     8101 gataaccagc gccgcttaag ccaggaacag ctacaacatg ctcaacaagt tgctgcccgt
     8161 gatttagtgc gtggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg
     8221 gcgcagctac tcagcaaagc attacctgac ccgtcactgc tggaatatac caaagtgatt
     8281 atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc
     8341 ggtacgcgcg ttaccgaaag tattcacaaa gtagctgaac gcgtggtaac gctggtgtcg
     8401 atggaactgc cggacaacgt gcggttgatt cgtgattacg atcccagcct gccggaactg
     8461 gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag
     8521 gcgctggggc cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc
     8581 ttacacggcg aacgctatcg gctggcggcg cggattgatg tggaagataa cgggccaggc
     8641 attccgcctc atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc
     8701 accgggcttg gcttatccat cgctcgtaat ttgattgatc agcattcagg caaaattgaa
     8761 tttaccagtt ggccagggca taccgagttc tcggtttacc tgcctatcag gaaataaagg
     8821 tgacgtttat gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg
     8881 tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac aacatttgag aacggcgcgg
     8941 aggtactgga ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc
     9001 cgggaatgga cgggctggca ctgctcaagc agattaaaca gcgccatccg atgcttccgg
     9061 tcatcattat gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg
     9121 cgtttgatta tctgcccaaa ccgtttgata tcgacgaagc cgtggcgctg gttgagcgcg
     9181 ccatcagtca ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga
     9241 ccgatatcat cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt
     9301 cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg
     9361 ctcatgccct gcatcgccac agtccgcgag ccaaagcgcc atttatcgct ctgaatatgg
     9421 cggctatccc gaaggatttg atcgaatcag aactgtttgg tcacgagaaa ggcgcgttta
     9481 ccggcgcgaa taccattcgt caggggcgtt ttgaacaggc tgatggcggt acattatttc
     9541 tcgatgaaat tggcgatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag
     9601 acggtcagtt ttaccgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg
     9661 ctgccactca ccagaatctc gaacagcggg tgcaggaagg taagttccgt gaggatctgt
     9721 tccaccgcct gaacgttatc cgcgttcatc tgccgccgct gcgtgagcgt cgggaagata
     9781 ttccccgcct ggcgcgccat tttttacagg ttgccgcgcg agaactgggc gtagaagcga
     9841 agttgctgca tccggaaacc gaagccgcgt tgacgcgtct ggcgtggcca ggcaacgtgc
     9901 gccagctgga aaacacctgc cgctggctaa cggtaatggc tgccggacag gaagtgttga
     9961 ttcaggattt gcctggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc
    10021 aaccggacag ttgggcgacg cttcttgcgc agtgggcaga cagagcgcta cgttccggtc
    10081 atcaaaatct gctttccgaa gcgcagccag agctggagcg gacgttactg acgaccgcgt
    10141 tgcgacatac gcaggggcat aaacaggagg cggcgcggct actcggctgg ggccgcaaca
    10201 ccctgacgcg taagttaaaa gagctgggga tggagtgatt cacggcttgt gtgtaaagat
    10261 tgattattga gcgcaaattg ctggtatttt gcgctttact gttccgataa gttcagtatg
    10321 atcttgcccg gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga
    10381 gtggcgcagt tgacagccac acaccgcaaa ctgctgttgc tgccgtgctg tgtgccgcga
    10441 tgattgggct gtttagttga gtttcagatt gctgatgaag tgctctttgt tcatgccaga
    10501 tgctgcgtga acgccttatc cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc
    10561 tgataagcgt agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
    10621 tactttgtaa acgaagcgcc attcactatt taaatcaccc gagagaactg ctgcatccgc
    10681 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
    10741 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
    10801 gcgagcagct tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
    10861 ataggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
    10921 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atccacttgc
    10981 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
    11041 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaaatta
    11101 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catccggacg ggcaaagtga
    11161 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
    11221 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
    11281 atggtcggca gatgcgcata gttaaagacg ctcagacggt cagggtttag ctccgccacg
    11341 cgtttcaggg taaaggcgaa actttccggc gtctgtttcg gcaggccgta aatcaggtcg
    11401 atgttggtgg aggtaaagcc aatctcacgc gcatggttaa gcagcgcaaa gataaactct
    11461 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccata
    11521 ctcaggcgat taaagccctc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
    11581 ggatcgactt cgatcgaaat ctccgcatcg gcattgaact ggaagttttc gcgcagcagc
    11641 ttcatcaggc ggctgatttg cgctttatta agatacgtcg gcgttccgcc gccccagtgc
    11701 agctggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
    11761 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcagccgcag
    11821 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    11881 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    11941 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    12001 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    12061 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    12121 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    12181 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    12241 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    12301 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcactca
    12361 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttctagta
    12421 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    12481 tgactttttc agtcacgccc aatggaatag gggttttact gccaatacgt ggatctttcg
    12541 gtgcgttctg gcctttgctg cctgacgtgt tgtttccgcc cgctgcgcgg ctgcccggcg
    12601 catgaccacg acgttttttc tgacgcttgc ggtcacgagc ttcctgattc agctcttcgc
    12661 gcgttttgcg acgcgctttt gcatgacctt tgctgcgtga tggtgaagat ggtttcataa
    12721 tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta acagaaagca
    12781 agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca taacccttaa
    12841 cgggtcttac aatccttgca ggccgccaac acaccctgtt tttccattag ccggaacaat
    12901 ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc tgacaatcct
    12961 ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct gttagcgtcc
    13021 tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc gccatcagta
    13081 tttgcacatt ttcagagttt tatataaaag caattaactg atttatatag cttatttgac
    13141 atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg tctcacaaag
    13201 cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta tggttcaggt
    13261 ataatcgccg caaagctttg actgacggag acgaccgctt tgactaattt gaattatcaa
    13321 cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga taccggaatt
    13381 gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact
    13441 aaccagaaaa gcctggctcg tacctcaaaa acccctggac gcacccagct tatcaacctg
    13501 tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc
    13561 ccggaagaga tgaagcgcaa atggcagcgc gcgctcggcg agtacctcga aaaacgtcag
    13621 agcctacaag gtctggtagt gctaatggat attcgccatc cgctgaaaga tttggatcag
    13681 cagatgattg agtgggcggt agacagcaat atcgccgtgc tggtactgct gaccaaagcg
    13741 gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga agcggtgctg
    13801 gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgc tgaagaaaca aggcgtggac
    13861 aagctgcggc agaaactgga tacctggttt aacgagatgc agcctgtaga agaaacgcag
    13921 gacggcgaat aattttctta ccttaatgct tatgccggat gtggcgtatc cggcccataa
    13981 attcaataga ttacaattct accgttggcc tgataaacat ggtgcatcag gcattacgga
    14041 tctttccttt cgcccaataa aaaacgcccc agtcattact gactggggcg gctaaaatat
    14101 tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct tacctctgta
    14161 ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt tgtaattttt
    14221 tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc ttatgttgct
    14281 tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc agttttcgcc
    14341 actccccact tccaccagca acggcacatc cagacgggta cagttttcca tcagttgatg
    14401 aatctgcttc gcaacggcat cgacatcatc tctatgaact tcaaatacca gttcatcgtg
    14461 tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat caacggcaat
    14521 catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg
    14581 ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat acagacggcg
    14641 tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc gttccatata
    14701 ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg cttctttacg
    14761 tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca gaccaaagtt
    14821 gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca aaccaaacac
    14881 ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca gcaagccttt
    14941 gtcacgcgag agatgcgcca taatgcgcag ttcaatctgc gagtagtccg ctgagacaat
    15001 cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt cgttacgcac
    15061 cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag ttacagcctg
    15121 gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca gcttgtcggt
    15181 gtaagtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg gcaacggata
    15241 gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac ccggcgtttt
    15301 cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct tggtggaaga
    15361 aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag ccagacgaag
    15421 ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac cgttacgttc
    15481 aatgcgtgaa agtactggca ccagcggcat ttcgatattc tcgaagacgt tcaacggccc
    15541 tttgtgtttt tgcagatccg gccacatttt cagatgcaac tgcaaggtga catcagcatc
    15601 ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaagtca gttgattttt
    15661 gcctttaccc gcaatctctt caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag
    15721 gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca gcatggtatc
    15781 aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat cgtatttcag
    15841 gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca actcgagtgc
    15901 acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg gaatatatgc
    15961 cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagctgaga tgttatcaag
    16021 gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg caatccacgc
    16081 tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca ccgttgccgt
    16141 cacttctggt gcttcgtctg caacactggt ttcctgtggc ttcgcggctg gttttgcccc
    16201 tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga actcatactt
    16261 tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt gttcacaggt
    16321 cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat aagcaacttc
    16381 tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca acccagcaat
    16441 tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca atgcctgcgc
    16501 ggttttttca ccgacgcccg gtacgccagg aatgttatca gaggagtcac ccatcagcgc
    16561 caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct cttccggtcc
    16621 gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca gctgcgccat
    16681 atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt cgcgcgccag
    16741 agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca gtcccatcgc
    16801 tttaaccatc gcgtgcaagg gttcgatttg tgctcgcaga tcgtccggca ttggcgggcg
    16861 atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg cgtcaaagac
    16921 caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt tgaggacacc
    16981 atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg cgtgatatgc
    17041 gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga tctgaaccat
    17101 aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg ataaaaacat
    17161 gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc tcgcaaatcg
    17221 tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt atcatgcttt
    17281 ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaatcca tccctaataa
    17341 ttgagataat gggatttttt ctaatgtgga tagatatgaa ttatttttct ccttaaggat
    17401 catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt tagcgcactg
    17461 tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc gataaccctt
    17521 ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc gatcatcatt
    17581 gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt ttcgcgtttt
    17641 tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca tctgaaccca
    17701 cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg gtatctgctt
    17761 atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt tcgtaagcac
    17821 attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt tcttggcctg
    17881 gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta tttattacgt
    17941 catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga aaagtttcgc
    18001 ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca agaaaaacat
    18061 cagcaaaccc actccacttt ccaaaacttg ttgccaccaa aggcggcagg cattgcgatg
    18121 gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgtt gtgttatccg
    18181 gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg gattgtcgta
    18241 catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa tgacaaaagc
    18301 tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga ccgacttctg
    18361 acgtcgttga tgtcatcaca gcggcaggac aagtaaattt tgctcgcctc tgaatgagca
    18421 gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat tcttcttcgc
    18481 tcatctcctg tccgttaaaa acaactttac ccggtgtata acgcagctgc agcgaggcgg
    18541 tattgttgtc aatcgtgatt aactgaaaca tctgacctaa tgtcatcatc ccgctgattt
    18601 gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa ttaagctgtt
    18661 ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc gatttgatat
    18721 ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc agattggcat
    18781 ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc tcactttgtt
    18841 gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga gcaacttcat
    18901 cgtttgctaa ttcagggtgt gcagcaagct gcttctgcat cgcaatgtta tactggatga
    18961 taaattggcg caccgctgag ggatcaatag attcagcaat caatgaccac tcaccactac
    19021 cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg acattgatat
    19081 aatctttgtc tttaacgcga tccagcctgg tttttgcaac gaaagcatcg atttgggcaa
    19141 cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt tcaccaaccg
    19201 gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt gccgttactt
    19261 tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata tccaggttag
    19321 acaatgaact actatcacca ttaaaagtaa actggccctc accaaaagaa aactttccat
    19381 actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg
    19441 tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc agttcattat
    19501 tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc attaacattg
    19561 tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg cctggcttga
    19621 tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacgg gaattaaaaa
    19681 agcctttgtc aaaattttga taacttaacg tcatatcgta ggcatgttca cctttctttt
    19741 tagcatcgtt aaaatcttta ataaattttt cgacaccagg ctgaatttgc gtacctgtgt
    19801 accatgtgcc accaccccag attacggcta acgcaacaat aacacc
//
LOCUS       DBFIFS010000065        19619 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.065, whole genome shotgun sequence.
ACCESSION   DBFIFS010000065 DBFIFS010000000
VERSION     DBFIFS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.065"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            78..578
                     /gene="tssB"
                     /locus_tag="N7A07_002156"
     CDS             78..578
                     /gene="tssB"
                     /locus_tag="N7A07_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU2476815.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            613..837
                     /locus_tag="N7A07_002157"
     CDS             613..837
                     /locus_tag="N7A07_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476816.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            888..2279
                     /gene="tssC"
                     /locus_tag="N7A07_002158"
     CDS             888..2279
                     /gene="tssC"
                     /locus_tag="N7A07_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU2476817.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2370..2783
                     /gene="tssE"
                     /locus_tag="N7A07_002159"
     CDS             2370..2783
                     /gene="tssE"
                     /locus_tag="N7A07_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU2476818.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2787..4637
                     /gene="tssF"
                     /locus_tag="N7A07_002160"
     CDS             2787..4637
                     /gene="tssF"
                     /locus_tag="N7A07_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU2476819.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4601..5683
                     /gene="tssG"
                     /locus_tag="N7A07_002161"
     CDS             4601..5683
                     /gene="tssG"
                     /locus_tag="N7A07_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU2476820.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5708..6988
                     /gene="tagH"
                     /locus_tag="N7A07_002162"
     CDS             5708..6988
                     /gene="tagH"
                     /locus_tag="N7A07_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU2476821.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            6985..7509
                     /gene="tssJ"
                     /locus_tag="N7A07_002163"
     CDS             6985..7509
                     /gene="tssJ"
                     /locus_tag="N7A07_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU2476822.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7512..8843
                     /gene="tssK"
                     /locus_tag="N7A07_002164"
     CDS             7512..8843
                     /gene="tssK"
                     /locus_tag="N7A07_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU2476823.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8848..9609
                     /gene="icmH"
                     /locus_tag="N7A07_002165"
     CDS             8848..9609
                     /gene="icmH"
                     /locus_tag="N7A07_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU2476824.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9618..12389
                     /gene="tssH"
                     /locus_tag="N7A07_002166"
                     /gene_synonym="clpV"
     CDS             9618..12389
                     /gene="tssH"
                     /locus_tag="N7A07_002166"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU2476825.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12386..13129
                     /gene="vasI"
                     /locus_tag="N7A07_002167"
     CDS             12386..13129
                     /gene="vasI"
                     /locus_tag="N7A07_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU2476826.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13134..14546
                     /gene="tssA"
                     /locus_tag="N7A07_002168"
     CDS             13134..14546
                     /gene="tssA"
                     /locus_tag="N7A07_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU2476827.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14683..18117
                     /gene="tssM"
                     /locus_tag="N7A07_002169"
     CDS             14683..18117
                     /gene="tssM"
                     /locus_tag="N7A07_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU2476828.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18128..19537
                     /locus_tag="N7A07_002170"
     CDS             18128..19537
                     /locus_tag="N7A07_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HGU2476829.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19503..>19619
                     /locus_tag="N7A07_002171"
     CDS             19503..>19619
                     /locus_tag="N7A07_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HGU2476830.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQI"
BASE COUNT         4577 a         5431 c         5457 g         4154 t
ORIGIN      
        1 cggcatttcc gatatttttt gcggtacgaa tatgccgtgt tcatggcctg tacgagataa
       61 catgacggat cacttttatg agcaaaaaat ttgaaggcag cgtcgcgcct cgcgaacgta
      121 ttaatatatc gtatgtccca aaaacagatg gccagacggc tgaagtggaa cttccgctta
      181 acatgctcgt tgtcggcgat accggaaata cgcaggaaac atcctcactg gatgaacgtc
      241 aggcagtttc cgttaacaag cacaatttcg gtgcagtgat ggctgaagca gccattggcc
      301 tcaattttac cgtccccgcc acgctgaagg gaagcacaac ggacgatgaa atgaatgtgg
      361 cgctgaatat caagtcactg gatgattttt caccagatag cgtggcccgg caggttccgg
      421 aagtgaataa attgctggaa ctgcgtgaag cactgactgc actgaagggg ccgatgggta
      481 accttccggc attccgtacc cagctccagg cattgctgga aaatgaagag tcccgcgagc
      541 agttgctcaa agaaatcggc caggtgagca ataaataacc accactattt cattaaacag
      601 ggttatccag gcatgacggc acacaaaaat atctccggga cataccacct ctctgttgca
      661 gacattctgc aggtggttta ccaggtatgt ttcagcccgt ccgttgaaat caaccaggat
      721 ggtgtggctg cgctgattac aacactggat cgccgtatca gtgatctatt ggatgagatt
      781 atccattttt gtgagtttca gcagagcgcg tcgcactggc agcgcgtact tcactgacgg
      841 cgaaaagccg gtttcagaaa cacagctaaa acagaaggat tttcagtatg tctttacagg
      901 aagaggaact cgtttccagt catgccgggc agcctgagca ggaatcgtcg ctgcttgatc
      961 aaatcatggc acagacccgt attcagcccg gttcggaagg ttatgacgtt gcccgccagg
     1021 gtgtgacagc ctttattgcc agcatcctgc aatccactgc atctgccgaa ccggtcaata
     1081 aactggccgt ggacagcatg attgcagata ttgatgaacg catcagccgc cagatggatg
     1141 tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt ctggcgctca ctgaaaacca
     1201 tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa cgtgcttcat gtcaccaagc
     1261 aggaacttct ggaagacttt gagtttgcgc cagaaattat tcagtccgga ttttataagc
     1321 acgtttattc gtccggtttc ggacagtttg gcggtgaacc catcgctgcc gtgctgggcg
     1381 cttatgagtt caaaaatacc gcgcctgaca tgaaactgct gcaatacgtc agtgccgtgg
     1441 gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc ggagtttatg gggctgaact
     1501 cgtggaccga actgcctaat atcaaagatc tttatgccat ctttgaaggt ccggcctaca
     1561 ccaaatggcg cgccctgcgt gactcggaag attcgcgcta tttagggctt acggcacccc
     1621 gcttcctgct tcgccagccc tattcaccaa cagataaccc tgttaagaac tttaactatt
     1681 acgaagatgt cagccagaac cacgaagatt atctgtgggg taatacggcc tggatgctgg
     1741 catgcaatat cgcagacagt tttgccaaat accgctggtg tccaaacatt atcggtccgc
     1801 aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt cgaaacgatg gggcagattc
     1861 aggccaaaat cccaaccgag gtgctggtca ccgatcgccg cgaatttgaa ctggctgaag
     1921 agggttttat cacccttacc atgcgtaaag actctgataa tgcagccttt ttctctgcaa
     1981 actcggtaca aaaaccgaag cactttccgg gaaaagatgc agaaaccaac tataagctgg
     2041 gtacgcagct tccttatctc ttcatcatca atcggttagc gcactacatc aaggtgttgc
     2101 agcgtgaaca actggggtca tggaaagaaa ggagcgactt agaaagagag cttaatacct
     2161 ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc agacgtgcgc agccgcaaac
     2221 ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg cgaacccggc tggtactagg
     2281 ttgcgctaag cgtgagacct cattttaaat ttatgggggc aaattttgag ctttccctgg
     2341 ttggccggtt agacagggag taacacacca tgatactgga tcgggaaaga accacaggtt
     2401 ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg gcagggaggc agtattcatt
     2461 cgctcaggca atccattcgc cagaacctaa gaaacattct taatacccgg tccggtagtt
     2521 gccggggtgc gcctgagctg ggcattgatg aaccggaagg ggctgaaaac ttccgcgaat
     2581 ccatgagtcg ggccatcgag caatgtatag aacgctatga accccggatc tcacacgctg
     2641 aagtgcaggc agtggtttca tccgcctcca gcccgctgga tatgacgttt catattacag
     2701 cctgggtgac atttaacgaa acgcatgagg tgcttgaatt tgatatggca ccgaatggca
     2761 gccagcatta ccgggtggac tgacaaatgg aatttgaaga acgctatttt cgggaagaac
     2821 tcgattacct gcgccagctt agcaagctgc tggcaacgga aaaaccccat ctggcccgct
     2881 tcctggccga aaaagatgcg gatccggata ttgaacgcct gctggaaggg gtggcttttc
     2941 ttaccggcaa tctccgccag aaaattgagg atgaattccc agaactgacg cacgggctta
     3001 ttaagatgct atggcctaat tacctgcgtc cggttccggc aatgaccctt attgaatata
     3061 cgccggatat ggataagtct tctgtaccgg tgttaatccc ccgtaatgag cagtttacaa
     3121 ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc tgatgctaaa aaggaggagc
     3181 cgcctccctg taccttcacc ctctgccggg atatctggct gctgcccgtt cgcctggagc
     3241 agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa catcaccttt tcggtcgcac
     3301 cgggaacgga cttccgcacg ctggatctga acaaacttcg cttctggctc ggcaatgacg
     3361 acaactatac ccgtgaccag ctttatttat ggttctgcga atacttgcag ggtgccgacc
     3421 tgactgtggg tgaacagcat attcgcctgc ctgagtttat gctaaaagct gtcggttttg
     3481 agccgcagga tgccatgctg ccctggccga aaaacgtcca cagcggctac cggatccttc
     3541 aggagtattt ctgttacccc gatgcgtttc tcttttttga tctttgtggt tgtccggctt
     3601 tgcctgacgg attgcaggcg gaattcttta ccctgcaact gcgtttttcg cgccctttgc
     3661 ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta ttgcgcacct gccattaatt
     3721 tatttatcca ccatgcagaa gccatcacgc tggacaaccg gcgggcagac tatccgctgg
     3781 ttcccagccg ccattaccca caacattacg atgtattttc cgttaacagt gtggtgagcc
     3841 aggtccagga tatgttcagg aaaaaagatc tggggcgtcc tgtttcgacg caggccgcgc
     3901 gccagtggcc agcctttgaa agtttcagcc atcagatgga atacagccgg aagcgggagg
     3961 tggtgtactg gcatcaccgg accaaaacat ccctgttcca tcgcggcttt gatcataccc
     4021 ttgcctttat acatgctgat ggcagttatc cgtcagacga atctctgctc agtaatgaag
     4081 tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc gtcacaaatt cgttccggcg
     4141 atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc ctttcgcaac attacccgcc
     4201 cgacgcaacc actctggccg gtcattgatg gcagcctgca ctggtccctg ctctccgcca
     4261 tgaacctgaa ttatctgtca ttactggata cggacgcgct gaagcaggtc atcgccaact
     4321 ttgatcgcca cgcaatccac catccgcaga cggcgcggct gtcacaacaa aagctggatg
     4381 ccattgagcg tctggagacc cgccccgttg atcgcctgtt tacgggtatt cccgtccggg
     4441 gactggcctc cacgctgtat ctgcacccgg agccgtttgt ctgtgaaggg gaaatgtatc
     4501 tgctcggtac ggtgctttcg cattttctgt cgctgtacgc cagcgttaac tcattccaca
     4561 tgctgaccgt tgtgaacaca gaaagccagg agacatggaa atggacggaa agaatcgggc
     4621 agcatcctct tatctgagtc cgggaaatcc cccggcagat aaagagcaga atgatccgct
     4681 ggcacaggtt ttccataatg cctgctctta caattttttt gcgatggcgg agctgttgca
     4741 ccgcctggca aagggtgaaa aggggacgcc agaattatcc ctgcgcgacg atccggcaca
     4801 ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc ccgtgcagtg atatcagtgc
     4861 gctgaaacgg gatacgtcag gggcattcag gatgaccact acctttatgg ggttacaggg
     4921 gagccagtcg cctctccccg gttattacct cgatcacctg gcctggaaag cggtacatga
     4981 acaaagtccg gtaggtgatt ttctggatat gttcagccac cgcctgaccc agtttgtctg
     5041 gcatatctgg cgtaagtacc gctatcacat cagttttcgt aacggcggtg tggatgcgtt
     5101 ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc cagcttcgcg ataagctggc
     5161 gattaatcac agcaaaatgc tggcgtattc cgggatcctg gcaaatccgg gacgatcccc
     5221 ggaaattatc tgtggcctgg tgtcccactg ctttgatttg tcagaagtga cgctgcaaaa
     5281 ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa aacagcctgg ggagctattc
     5341 tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg ggcaactttg ttctgggtac
     5401 acgagtaccc gatctcagcg gaaaattcca gctcagtatt accagcctga cccgaaaaca
     5461 gttcctctct tttctgccgt ccggcgaaaa cttcctgcca ctgacgacgt tcgtgtcctt
     5521 catcctgcgc gatcagcttg cctgggactt acatctgggg ctggcgccgg agcaggtggg
     5581 cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc tggaccagct tcctcggcac
     5641 accggaagaa cgaccctcag tcaccatcag ggtacggtcg taaattatga taatcacgga
     5701 gcagaatatg ccggaagaga aactacagac gctttcattg caggtcatta acggcagtga
     5761 gctggaaagc gggcgggcgg cgcgctgtct gttcacacag cagggaaatg tcggccatgc
     5821 ccccgaatgc cactggtcgg tacaggatcg tcagcagagc attccggccc aggcttttac
     5881 cgttatcctg cacgatggca cattttgtct acgcccgcag acggcacaac tgtggctgaa
     5941 tcaggcaaaa gtcacagcaa catcagacct gatacagttg cgccagggcg atgagatcca
     6001 gatcggacgg ctgatggtga gggttcatct gaaccgggga gatattcccc attacgatga
     6061 ggaaatggcc actcccgaaa ccatcgttac caatcgcgat atgctcacgg ataccctgct
     6121 atcaacggag ggtgcgccac actatccggg aatgactcac cggcaccagc ttgcagacac
     6181 cgtggtaaat ggtttttctg ccgatccact ccaggcactt cagtccgaaa gcctgattac
     6241 cacgggcgat ccgctttcag gcattgcggc tgtccggcca tcggcaccgc tgtccgatcc
     6301 ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt atgccagccc
     6361 tggcgatcat aatgatgaca tctctgcggc agaaatggcg caacgccacc ttgcggtcac
     6421 ccccttactg cgcggtcttg gcggctcgct taccgtgagc aattccgacg atgcggatga
     6481 ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc tcgatttgca
     6541 gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata acccgctgcg
     6601 cctgaacatg gattacgcca ccgcgctcga cgtgatgttt gccgaaggta aaagcccggt
     6661 acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc accacgaaga
     6721 agctaaccgg gcggcgattg tggagtcgct tcgtgtcctg ctggatgctt tctcaccaca
     6781 aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc agccgctgga
     6841 tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct ccgatcgcca
     6901 gcaggggttt gagatgctgt ttaacgaggt ctacgcccag gtctatgacc gggtgcttcg
     6961 tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg cgtgctgatg
     7021 tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gcgaggtgat caaaaacccg
     7081 gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac cctgctgact
     7141 gaaccggaca ccaataccaa tgcggagggt gaatcagccg ccgtggatgt gcaactggtg
     7201 tacctgactg acgactcaaa attgcaggca gccgactatg accagatagc cagtacgccg
     7261 ctaccggatg tgctgggtaa aaactatatc gaccaccagg atttcaacct gttgccggac
     7321 accatcaaaa ccctgccgcc gataaagctg gatgagaaaa cgcagtttat tggtgtcgtg
     7381 gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt ggaaggaacc
     7441 ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat gaaaaaagag
     7501 gatgaataat tatggcaacc acccgcaaca aggtgatgtg gcaggaaggg atgctgatgc
     7561 gcccacacca tttccagcaa cagcagcgtt acaacgacta cctggataac cagcgtttcc
     7621 gggccatgaa tgatttatcc tggggattta ccgaactcac cctcaacaat gaactgctgg
     7681 cgcagggtaa gatcatgatt gacagcgcgt caggcacact gcctgacggc accgtctttt
     7741 ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaaaatttt ccggacgaaa
     7801 gaagccgcaa tatttacctc gctctgccgg tcgccagtga tgtgagaaat gaaatcagcg
     7861 acgggcggcg aatcgggcgt taccggctga attatgccga tgtccgggat ctgcattcag
     7921 aagaaggcga cgcgcgaaca ctgacgctgg gacaactgac gccgcgcatt atgagcggtg
     7981 cagaagatat gagtgcctat attacgctcc cgctttgccg tatcagcgat cgccatgccg
     8041 acggctccct gacgctggat gacgatttta tcccctcctg ccagaatatt caggtcagta
     8101 agaaactgcg tgtttatctc aaagaggtac agggggccat tggcggacgg gcaagcgatc
     8161 tggcaaaccg tattggctct ccggcgcaga gcggcatcgc ggatgtggcg gaatttatga
     8221 tgttgcagtt gcttaaccgt aaccagaccc ggtttaccca tcgcgcccgc cgatcccagc
     8281 tccacccgga agatttctac cttgatcttg ccgagttgct gggtgaactg atgaccttta
     8341 cagagccgtc acgcctgccc tgcccgcttg atgtgtatga tcatcatgac ctgaccaaaa
     8401 catttaaaac actgttaccg gaagtcaaac gggcgctgca taccgtactg tcgccaagag
     8461 cggtcaatct gccgctgcat ctgcgtgacg gcatctggca ggccgatgtt catgactcgg
     8521 aactgctaca gtctgccacc tttgtactgg ccgtggcagc aaatatgcct gtcgatcaga
     8581 tccagcgtca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc cgcaatatgg
     8641 tcagtgtgca gatcccgggt attcctctgc gcgcactgat ggtggccccc cgccagcttc
     8701 cctaccattc cgggttcagc tatttcgaac tcgacaagag cggccaggcc tggacagaaa
     8761 tggctgccgc cggggccgtt gcactgcatg tatccggcag tttcccggat ctgaacatgc
     8821 aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct gccaccattt
     8881 accggatacg acgaaaaaag cctgcgcgac taccatctcg cgcttcatgg caacagcctg
     8941 aatccgatga tcgacgcggc gacgccgcta ctcggtatgg taatgcgcct ttccaccatg
     9001 aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacagatgt tcaggccgtg
     9061 gagcaactgc ttcaggaaca gggctacgaa ccgggcgtga ttatctcgtt tcgctacatc
     9121 ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcgaacaa gaacgagtgg
     9181 atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggcga gaaagttttc
     9241 atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct ggaatttctc
     9301 tggctctgtt tttctctggg atttcgcggg cgctacaaag tggcagtaca ggatcagggg
     9361 gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg cggcgatgcc
     9421 cctttcccgc tgttacacca ggacaaaaaa acccaaggcg ggcgttatca gctcatcagt
     9481 cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt gttttacctg
     9541 ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct aaacaaatta
     9601 cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa cggctgaacc
     9661 tgttctcccg ccaggcgctg gagatggccg cctcagaatg tatgagccag caggcagcgg
     9721 aaattaccgt cagccatgtg cttattcaga tgctcgccat gccacgcagt gacctgcggg
     9781 ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgttg acggtagaga
     9841 actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg ctggttgagt
     9901 ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca gcacagcgaa ctgcgcggcg
     9961 gcgtgttgct gctggccttg ctgcattcgc cgctgcgtta tataccgcct gctgccgccc
    10021 ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag tggacacagg
    10081 agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca ctgacggacg
    10141 cctctgacac cctgcttgcc cgctacgcca aaaacatgac agaagacgcc cgtaacggca
    10201 ggctagcccc agtactgtgc cgcgaccacg aaatcgacct gatgatcgac attctctgcc
    10261 gccgccgtaa aaacaacccg gtggtggtgg gcgaagcagg cgtgggtaaa agcgcgctga
    10321 ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaaactg aaaaacaccg
    10381 atatcatgac ccttgacctg ggcgcattgc aggccggggc atcggtgaag ggtgagtttg
    10441 aaaaacgctt caaggggctg atggcggagg tcatttcctc tccggtgccg gtcattctgt
    10501 ttatcgacga agcgcatacc ctgattggcg cgggcaacca gcagggcggg ctggatatct
    10561 ccaacctgct gaaaccggcg ctggcgcgtg gcgaactgaa aaccatcgcc gccaccacct
    10621 ggagcgagta caaaaaatac ttcgagaaag acgccgccct gtcgcgccgc ttccagttag
    10681 tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgtggt ctgtcggcgg
    10741 tctatgaacg gtctcacggc gtgctgattg acgatgatgc cttgcaggcc gcagcaacgc
    10801 tgagcgaacg ttatctctcc gggcgtcagt taccggacaa agcgattgat gtgctggata
    10861 ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc tcggcgctga
    10921 ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag cttcgcatcg
    10981 gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat gaaacgctga
    11041 cggcgctgga tgaactggaa gcggcttggc accagcagca gacgctggtc cgggagatta
    11101 tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg ttgccggacg
    11161 cagatactgt ggaggatacg cagccagagt cagagtcaga gtcagaacag gataataccg
    11221 gtgccgtacc ggctgatgag accgacagag aacagccgga agagaccgct gaaacagttt
    11281 ccccggtaca gcggctggcc cagctcactg ccgaactgga cgccctgcat aacgaccggt
    11341 tgctggtctc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc gaatggaccg
    11401 gcgtgccgct caaccgcctg tcgcagaatg aaatgtcggt catcaccgac ctgccgaaat
    11461 ggctgggcga caccatcaaa ggccaggacc tggcgattgc cagcctgcat aagcatctac
    11521 tgaccgcacg cgccgacctg cgtcgtccgg gacgcccgct cggtgcgttc ctgctcgctg
    11581 gccccagcgg cgtgggtaaa accgaaaccg tcctgcaact ggcagagctg ctctacggcg
    11641 gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggagaaacac accgtttcgc
    11701 ggctgattgg ctcccctccg ggctatgtcg gctatggcga aggcggcgtg ctgactgaag
    11761 ccatccgcca gaaaccctac tcggtggtgc tgctcgatga agtggaaaaa gcgcacccgg
    11821 atgtcctcaa cctgttctac caggcgtttg acaagggcga gatggccgac ggcgaaggcc
    11881 gcctgattga ctgtaaaaac atcgtcttct tcctcacctc caacctcggt taccaggtga
    11941 ttgtcgaaca cgcggatgac ccggaaacca tgcaggaagc cctctatccg gtgctggcgg
    12001 acttctttaa acctgccctg ctggcgcgaa tggaggtggt gccgtacctg ccgctgtcga
    12061 aagagacgct cgccaccatt atcgccggga aactggcccg tctggataac gtgctgcgca
    12121 gtcgctttgg tgcagaagtg gtcattgaac cggaagtgac ggacgaaatc atgagccgcg
    12181 tcacccgcgc ggaaaacggc gcgaggatgc tggaatctgt catcgatggc gacatgctac
    12241 cgccgctctc gctgctgctg ttgcagaaaa tggcggctaa cacggcgatt gcccggattc
    12301 ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag aacgacgagt
    12361 ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc agacaggatg
    12421 gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc
    12481 aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga cagccacgtt
    12541 acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg actgctatga
    12601 ccacctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca aagccggttt
    12661 tgtgggcgag gcctggaccc gcgccacaga gcaggaaaaa cgccgcgagg gcaacaccac
    12721 agaactgctg gtgacgcagg tgccggggga gcgcccgacg gtggtgataa ccaccccggc
    12781 tatcggccat gttccgccgc gcccggtgct gatgttcagt tgcgtggaca atatcacccg
    12841 gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca ctctgaatgc
    12901 cgacagccgg gcattgcgca gccactggtt tgtgcgcgag aacggcaccc tgctggagtc
    12961 cagccggggg ctgtccggga ttgatgaaat taagcaactg ttcggcgcaa aaacgctgac
    13021 agtggatacc ggcgcagata acgccgcagg aaagctgacc tttaacattg acgggctggc
    13081 aagggcgatt gcccccctgc gcgatgcctg ccactgggcg ggagaataaa aatatggcaa
    13141 tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg ccggaagaaa
    13201 aactggcatc ggcgctgaaa gatgacaacc cggactggga gtatatcgac ggcgaaatcg
    13261 tcaagctggg gtctctggcc cacgctcagc ttgatattcc cgaactacaa cgcagggggc
    13321 tacagcttct ggcttctgaa agcaaagact tcaggctact ggcacacctg ctcagaaccc
    13381 tgcaacatgc cggtgatcca ctgctggcac tgcacctgct aacgctatac gtggaacatt
    13441 actggactgt ggccgcgccg cagaatatgg cgcataaaaa acgctttgcc agccagatca
    13501 ttaaacgttt tgaaacgggt attgaaggct tttcacaaaa cgctgccaca acgcagcgcg
    13561 atactctgct gggtgagctg gcgaaactgg cgcagtgctg gcagtcacat aacgtcccgg
    13621 aactggcaca ggctaccgat gatctttttg ccctgtacca gcgtacgttt aatcgtgcgg
    13681 ctcctgctcc ggtccccact ccggcggcct ccggtagttc accacaaacc accgtcacgt
    13741 ctgaaagcgg cgtgacgcaa cccagtgctc cggctcccca aatcgccatc gacagtcacg
    13801 acgacaaagc ctggcgcgac acgctgttaa aagtggcggc tattttatgt gaacgccagc
    13861 cggactcgcc gcagggctat cgcctgcgcc gccatgccct gtggcaatcc atcaccagta
    13921 caccccaggc ggaaagcgat ggacgtaccc cactggctgc ggtctctgcc gatatggtgg
    13981 cggattacca gtcccggctt gccagcgcgg atatggcgct gtggcaacag gttgagaaaa
    14041 gcgtattgct ggctccttac tggctggacg gtcactgtct ttctgcacag acggcactgc
    14101 gtctgggtta caaacaggtg gcagacacca tccgcgatga ggtcatccgc ttccttgagc
    14161 gtctgcccca gcttaccggg ctgctgttta atgaccgcac accgtttctc agtgagcaga
    14221 cgaaacaatg gctggctgct tcgcccgacg gcaaagttgc accggttgcg caaatcggtg
    14281 aggaatcgca ggcagccaga gcctgttttg ctgggcaggg tctggaggcg gcgctgcgat
    14341 atctggacat gctacccgaa ggcgatcccc gcgatcagtt tcaccgccag taccttgccg
    14401 cacagttgac ggaggaggcg gggctgatac agcttgcgca gcaacagtac cggatgttgt
    14461 tgatgatagg gagtcagatg atggtgtctg actgggagcc atcattactt acgcagcttg
    14521 aacaaaaatt cacggcagaa caataaaaga gaacaggagt ttttgtgttc agattaccaa
    14581 caccccgact actcagcgga ctcaaatcag ccctgcgacc ggcgatgccc cggtttaaag
    14641 tctccgcttt ctggctgctg atactggcgt ggatttttct gcttgctgat actggcgtgg
    14701 atttttctgc tggtatggat ctggtggaaa ggcccgacgt ggacgctgta tgaagaacag
    14761 tggctcaaac cactggcgaa ccgctggctg gcaacggcgg cgtgggggat tattgccctg
    14821 gtgtggctta ccgtccgggt aatgaagcgc ctgcaactgc ttgaaaaaca gcaaaaacag
    14881 cagcgcgaag aggccattga tccgctcagt gtggagctga acgcccagca gcgttacctt
    14941 gaccgctggc tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagtta
    15001 ccgtggtata tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcgagggg
    15061 tttccgtcag acattattta tgccccggag ggcgcgcgcg gcacagaaca acgcctgtac
    15121 cttacgcccc atgtcggtaa acaggcggtg atctttgata tcgacggcac actctgcgct
    15181 cccgctgatg cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa
    15241 gaaaagcgcg cgcgccagcc gctgaacggg attattctga cactcgattt acccgatctg
    15301 ctgaccgcag acaaacgcca ccgcgagcat ctgttacaga cgctgcgcag ccgcttacag
    15361 gatatacgcc agcatcttca ctgccagtta ccggtttacg tggtactgac ccggcttgat
    15421 ttattacagg gttttgccgc cctgttccag tccctgaaca gacaggatcg cgatgcgatt
    15481 ctgggcgtca cgttcacccg ccgtgcccat gaaaatgatg actggcgaac agagttgaat
    15541 gctttctggc agacatgggt ggatcgaatg aatctggcgt tgccggatct gatggtcacc
    15601 cagactcaca cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg aagccgtgaa
    15661 ccgctggtgt cactgcttga gggtctgctt gacggtgaaa atatgaacgt gatgctgcgt
    15721 ggtgtctatc tcacctcttc gcttcagcgc gggcagatgg atgatatatt cacccagtct
    15781 gccgcccgcc agtaccgact gggcaataac ccactggcgt cctggcctct ggtggacacc
    15841 gcgccttatt tcacccgcag cttgttcccg caggcattac tcgcagagcc taatctggca
    15901 acggagagcc gcgcctggct gatgcgttcc cgtcgccgcc tgacggtttt ctctgccacc
    15961 ggcggcgtgg cggcactgct gctcatcacc ggctggcatc actattacaa cggtaactat
    16021 cagtccggca tcaccgtgct taagcaggcc aaagccttta tggatgtgcc gcctccacag
    16081 ggggaagatg actacggcaa cctgcaactg ccgctgctga acccggtacg cgatgccaca
    16141 ctggcctatg gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga
    16201 cgacgtatcg gaccttatgt ggaacagacc tatctgcaac tgctggagca acgttacctg
    16261 ccctcgctgt ttaacgggct ggttaaagcg atgaatgccg cgccgccgga gagtgaagaa
    16321 aaactcgcgg tgctgcgcgt gatgcgaatg ctggaggaca aaagcggacg taacaatgag
    16381 gtggtgaagc agtatatggc aaaacgctgg agcgaaaagt tccacggcca gcgcgatatc
    16441 caggcacaac tgatgtccca tcttgactac gcgctggctc atactgactg gcacgcagag
    16501 cgtcaggcgg gcgacggtga cgccatcagc cgctggacgc catatgacaa gcccgtggta
    16561 tcagcacaga aagaactgag caaactgcct gtctaccagc gggtttacca gagcctgaaa
    16621 acgcgggcgc tgggcgttct tcctgccgac ctcaatctgc gtgaccaggt agggccaacc
    16681 tttgaccagg tgtttacatc tgccgatgac aacaaactgg ttgttccaca gtttcttacc
    16741 cgttacggcc tgcaaagcta ttttgtaaaa cagcgcgatg aactggttga actgacggcg
    16801 atggattcct gggtacttaa ccttacccgc agcgtgaaat acagtgacgc cgaccgcgcg
    16861 gaaatccagc gccagctcac cgagcagtat atcagcgact acaccgccac ctggcgggcc
    16921 gggatggaca atctgaatat ccgcaatttt gagtccatcg gacaactgac cggggcgctg
    16981 gagcaggtta tcagcggcga ccagcctttg cagcgggcgc tgaccgtgct gcgtgacaac
    17041 acacagccag gcgtcttttc tgaaaaactc tctgccaaag aacgggatga ggccctggca
    17101 gagccggact accagttact cacccgtctc gggcatgagt tcgccccgga aaacattacc
    17161 ctggcagtac agaaagacaa agaaagcacg atgcaggccg tgtatcagca actcaccgag
    17221 ttgcaccgct acctgctggc aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa
    17281 gccgtgcagt tacggcttga tcagaacagc agcgatccga tattcgccac ccgccagatg
    17341 gcaaaaacgc tgcctgctcc gctcaaccgc tgggttggca gactgactga ccaggcctgg
    17401 catgtggtga tggtggaggc tgtacattat atggaagtgg actggcgcga cagcgtggtg
    17461 aaaccgttta acgagcaact ggcaaataac tatccgttta atccgcgttc tgcacaggat
    17521 gcctcactgg atgccttcga acgcttcttt aaaccggatg gcatactgga taccttctac
    17581 cagcagaacc tgaagctgtt tatcgataat gacctgagtc tggaggatgg cgataacaac
    17641 gtcattattc gcgaagatat tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc
    17701 ttcttcagca aacagaacgg tctgggaaca tcctttgccg tggaaacggt atcgctttca
    17761 ggcaataaac gccgcagtgt actgaacctt gacggtcagt tagtcgatta cagccagggc
    17821 cgtaactata ccgcccatct ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag
    17881 ctgacgctca tcggcaccag cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg
    17941 gcgcagttcc gcctgttcgg ggccggacaa ctgaccggag tacaggatgg caactttacc
    18001 gtgcgcttta gcgtggacgg tggcgcgatg acctaccgtg tgcataccga cacggaagat
    18061 aacccgttca gcggtgggtt gttcagccag tttggtctgt cagacacact gtactgagga
    18121 ggcagggatg aatagtaacg tactgacaca aactatcgtt accggcagtg acccgcgcgg
    18181 gctgccggaa ttcagcgcca tccgcgagga aataaacaaa gccagccacc cgtcacagcc
    18241 tgagctgaac tggaaactgg tggagtcgct ggcgctggcg atttttaaag ccaacggtgt
    18301 ggatttacac accgccacct actatacgct tgcccggaca cggacacagg gactggcggg
    18361 attctgcgaa ggtgcggaac tgctggcggc aatggtaagc cacgactggg ataagttctg
    18421 gccgcagggc ggcccggcgc gtactgaaat gctggactgg tttaactccc gaaccggcaa
    18481 tattctgcgt cagcaaatct cctttgcgga atccgaccta ccactgatct accgcacaga
    18541 gcgggcattg cagcttatct gcgataagct ccagcaggtg gaactgaagc gcgttccgcg
    18601 cgtggagaat ctgctctatt ttatgcagaa cacgcgtaaa cggcttgaac cccagctgaa
    18661 gagtaacact gagaacgccg cacagaccac ggtcagaacg ctgatttatg ccccggaaac
    18721 acaggcatct tccacaccag aagcggtagt gcctcccctg cccggcctgc ctgagatgaa
    18781 agtggaagtg cgcagtctga cagagaatcc cccacaggcc agtgtgataa agcaaggcag
    18841 tacggtaaga gggtttatcg cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg
    18901 gtggtggcag gtctatccgg tgcagcagca actgttacag gttaacgaca ccgctcaggg
    18961 cgcagcaacg gtgtggatgg cctcacctga actcgaaaac tatgagcgca ggctgcaaca
    19021 acttcttgat acctccccgg tacagccgct ggaaaccggg atgcagatga tgcgtgttgc
    19081 cgacagtcgc tggccggaaa gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc
    19141 actcaaaacc cgcgcacaga gtagcccgca gttgcgtggc tggttgcaga cccgccagga
    19201 cttacatgct tttgcagatc tagtgatgca gcgcgagaaa gagggactaa ccctttccta
    19261 tatcaaaaat gtcatctggc aggcggagcg gggactgggg caggaaacac ccgttgagtc
    19321 tctgttgacg cagtaccagg atgcccgtgc gcagaagcag aatacagatg cgctggaaaa
    19381 acaaattaat gagcgactcg aaggcgtgtt aagccgctgg ctgctgctga agaataatac
    19441 gataccgacg ataaaaaaag cattgaattt caataacatt catgaataca aaggagtttt
    19501 aaatggcgaa tttaatttat ttaacactaa atggtgataa tcaagggctc atttcaagtg
    19561 gttgttcatc acaaccatcc attggaaata aagctcaaac agcacacatt gatcaaatt
//
LOCUS       DBFIFS010000066        19460 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.066, whole genome shotgun sequence.
ACCESSION   DBFIFS010000066 DBFIFS010000000
VERSION     DBFIFS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.066"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..617)
                     /locus_tag="N7A07_002172"
     CDS             complement(<1..617)
                     /locus_tag="N7A07_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU2476831.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(688..2802)
                     /gene="fusA"
                     /locus_tag="N7A07_002173"
     CDS             complement(688..2802)
                     /gene="fusA"
                     /locus_tag="N7A07_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU2476832.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="N7A07_002174"
     CDS             complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="N7A07_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU2476833.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="N7A07_002175"
     CDS             complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="N7A07_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU2476834.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="N7A07_002176"
     CDS             complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="N7A07_002176"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU2476835.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="N7A07_002177"
     CDS             complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="N7A07_002177"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU2476836.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="N7A07_002178"
     CDS             complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="N7A07_002178"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU2476837.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="N7A07_002179"
     CDS             complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="N7A07_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2476838.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="N7A07_002180"
     CDS             complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="N7A07_002180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2476839.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6928..7146
                     /gene="slyX"
                     /locus_tag="N7A07_002181"
     CDS             6928..7146
                     /gene="slyX"
                     /locus_tag="N7A07_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU2476840.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="N7A07_002182"
     CDS             complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="N7A07_002182"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU2476841.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="N7A07_002183"
     CDS             complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="N7A07_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU2476842.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8117..9895)
                     /gene="kefB"
                     /locus_tag="N7A07_002184"
     CDS             complement(8117..9895)
                     /gene="kefB"
                     /locus_tag="N7A07_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU2476843.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="N7A07_002185"
     CDS             complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="N7A07_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU2476844.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10577..12490
                     /gene="yheS"
                     /locus_tag="N7A07_002186"
     CDS             10577..12490
                     /gene="yheS"
                     /locus_tag="N7A07_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2476845.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12490..13512
                     /gene="yheT"
                     /locus_tag="N7A07_002187"
     CDS             12490..13512
                     /gene="yheT"
                     /locus_tag="N7A07_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU2476846.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13506..13724
                     /gene="yheU"
                     /locus_tag="N7A07_002188"
     CDS             13506..13724
                     /gene="yheU"
                     /locus_tag="N7A07_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU2476847.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13778..14647
                     /gene="prkB"
                     /locus_tag="N7A07_002189"
     CDS             13778..14647
                     /gene="prkB"
                     /locus_tag="N7A07_002189"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU2476848.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="N7A07_002190"
     CDS             complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="N7A07_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU2476849.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15408..16040
                     /gene="crp"
                     /locus_tag="N7A07_002191"
     CDS             15408..16040
                     /gene="crp"
                     /locus_tag="N7A07_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU2476850.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16091..18181
                     /gene="yhfK"
                     /locus_tag="N7A07_002192"
     CDS             16091..18181
                     /gene="yhfK"
                     /locus_tag="N7A07_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU2476851.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18248..>19460)
                     /gene="argD"
                     /locus_tag="N7A07_002193"
                     /gene_synonym="dapC"
     CDS             complement(18248..>19460)
                     /gene="argD"
                     /locus_tag="N7A07_002193"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU2476852.1"
                     /translation="EQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEYVDF
                     AGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERVVFM
                     NSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDGFGP
                     KPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQHQA
                     LLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAFHPG
                     SHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSDIRG
                     MGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEGMQR
                     FAHAVAKVVGA"
BASE COUNT         4759 a         5331 c         4839 g         4531 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaggaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcacgagcag caccgccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta
      541 gttttaccgt ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca
      601 aatttttctt tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc
      661 acgggacttt ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc
      721 aacgttactc ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt
      781 ggtcagagaa cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg
      841 gatcttaacg ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa
      901 gtcaccgata acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg
      961 ctcaagcaga actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag
     1021 tttaaacgcc agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc
     1081 catgtctact accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt
     1141 atcaacggcc gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc
     1201 gtagcctttc gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata
     1261 ctgaccacga ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg
     1321 gatagtttca cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg
     1381 cttcatacgg tcaacgatga tgtcgaggtg cagttcgccc atacccgcaa tgatggtctg
     1441 gttagattct tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag
     1501 agccagaccc attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac
     1561 cggctcaggg aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc
     1621 accagtggtt acgtctttca gaccgatagc agcagcgatg tcgcctgcgc gaacttcttt
     1681 gatctcttca cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc
     1741 tttcacggag ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa
     1801 ggtcaggtta ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc
     1861 gtcatcactt gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat
     1921 cgcaggtacg tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac
     1981 acctttgttc ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg
     2041 ctgacgcaga gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc
     2101 catcagctct tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc
     2161 cagttcaacc atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc
     2221 gttccagttg atagctttca ttttcaccag gtcaacaaca ccggtgaaat gttcttcagc
     2281 accaatcgcc agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac
     2341 aactttcagg aagttcgcac ccatacggtc cattttgtta acgaacgcaa tgcgcggaac
     2401 tttatatttg tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc
     2461 gcagtaaacc attaccgcac catcgagcac acgcatggaa cgttctactt cgattgtgaa
     2521 gtcaacgtgc cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat
     2581 accagaccag aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg
     2641 ctccatccag tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc
     2701 ggtgtagaac agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat
     2761 accgatgtta cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct
     2821 tttaggcgtt caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg
     2881 actaaaactc cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat
     2941 acggtgaacg tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag
     3001 ttcgttcgcc aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat
     3061 ccaacgcatt gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt
     3121 agaaccacca acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac
     3181 ttcgaatgct tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta
     3241 tacgatagat tcagcagtag attttttacc atctaccatc aggatattta caaatttagc
     3301 cagcagttct gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg
     3361 acgtggcatg gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt
     3421 ttgacattta agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg
     3481 cttcacgcca tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc
     3541 accacgtacg gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag
     3601 gatcacggag tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa
     3661 accgttagtc agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt
     3721 ggtagtatat acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac
     3781 gttgcttttc gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat
     3841 taaatagctc ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa
     3901 tatgaggacg ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc
     3961 cgccatcacc aggccatctg gccggagtgc ttaaccgtaa gtctgacgaa atcagtatag
     4021 tcaatgggaa tgatgtcgtt cgaaatttga ccagtcaaac cgcgggcaat aaggtcttcg
     4081 ttcagggcat agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca
     4141 actgcggcag ttacgccatc ttgcaataat agcagttcgt cgccttcact gagcagacgc
     4201 agcagcgcag caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc
     4261 tcaaaacctc aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag
     4321 cggcgtggct tcgacaacaa agggggtctg cggatctaac ccgcgttcgc gcagtgaagc
     4381 cgcacaaacc cagcactgtt caatgtcgta cagatccaac aatttaaaag tggcaatgta
     4441 atcacgcgcc agcactgcat cgggcttttg tcctggcagt agctgaaaaa cgccatcagc
     4501 aataaagaag acggccagct cgtcagttaa cgcggaagtt gccagtaaag catctaaacc
     4561 ttcccgcccg gcagcggtac catgaggcac agtagaaaaa acaaacgcaa ttcgtttcat
     4621 cagaactgta ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt
     4681 aaggtaaatc cctgctgaag gtttgacgaa gccagcccca gtcttccggc ctccatttca
     4741 tcaacaacgc cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc
     4801 gcattcaact gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaag
     4861 tggttagcgt tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct
     4921 gctatcagcg cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc
     4981 ccggtcacca cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga
     5041 actggcggat gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt
     5101 tgatcgcatc tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat
     5161 ttttggcgtt attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg
     5221 tttgggtaac cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg
     5281 tttctggcgg cacaaaggtg ctcataatct gcgagaaggg aacatcaaga ttcatattga
     5341 tgcacagcag accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca
     5401 ttaatacgcc gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg
     5461 tcatatcgtg cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg
     5521 tatgttcacc gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa
     5581 cgatttcaca gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt
     5641 tcagaatatc aaaatcggtc tgctcgaaag gacgttgatc cagtaaatcc aactcactgg
     5701 tttcgttggt taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg
     5761 ttaacttttc agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt
     5821 ccgctttcca gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg
     5881 gcggttctta atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt
     5941 gcatcagcct tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa
     6001 ttcggtggga tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt
     6061 ttgatcttac cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg
     6121 tccagacgga aagagagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg
     6181 atcagcgtac ctttgtagtt cacgacaaca gtatcgctgt ctttcggtgc ttcgccttta
     6241 ccggcttcta ctacctgata aaccagacca gtggaagagg ttttcacacc tttctctttg
     6301 gcaaatttct cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc
     6361 ttcgcctgag cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct
     6421 tggtcggaga gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct
     6481 ttatccagtt tgatgcccag tttttcttgt tcttttagag agttttccat gtaacgaccc
     6541 agcgaggcac ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg
     6601 tcagcagttg tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcaaggca
     6661 acggccattg tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg
     6721 atcggggcat ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga
     6781 acaaatctac atacagacac gccctattat catctacttt cagactcttt ttgtttaaat
     6841 tagtttcgat gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg
     6901 gcgaccattc gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca
     6961 gagctggaga gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg
     7021 accgctcatg aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag
     7081 ttaaaagcca gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat
     7141 tattgaggcg taaaaaaagc ggggattccc cgcttttttg tcaccttttc ggtattagtg
     7201 gcaaccgcaa ccgccgttgc ctttaccgcc acagcagcct tcgccaccgt gttcatgacc
     7261 gtgatcgtgg ccatgaccgc cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg
     7321 cgcgccgtga acgtggccat gagccagttc ttcttcggtc gcttcgcgaa tcgccacaac
     7381 ttcaacgttg aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccacgacgtg
     7441 atcgtcttca accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa
     7501 acgcatacct acctgcagtt catcaacgcc cataaatacg tctttaggaa cacgttgcac
     7561 caggttttcg tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc
     7621 gccaacttca tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg
     7681 caggtagtcc agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg
     7741 tacctgatag gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca
     7801 tgggaagaat agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa
     7861 aaaaactcgg cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc
     7921 gaacgtgatc gcgggcttct ttgtctgctt ctcgcatctg gtgtccgcac ttaacgcatt
     7981 caacaatatc aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacaggccg
     8041 ggcattttgc gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt
     8101 catcccagcc gtccagctag cgtcgttctt gttgcatttc acgctggaaa atctcttcca
     8161 gttcgcgtcg ggcttccctg gcgcgagaaa tttgtacggt atcggcatgc attgggataa
     8221 gctctcgcag catccgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat
     8281 gcggatgcat accaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg
     8341 tttcacggga aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc
     8401 gcgctcgcgc aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca
     8461 tggtgtcttc cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag
     8521 aacgtaaaag atctacctgc gtggcatcac cgtaataaac tttgtagcca tatttgcgca
     8581 tcaggttaac ggcgctgata tcccgctcca gcacagtaat gcgcatttta tttgccatca
     8641 acaaacgacc aatcacctgc ccaaaacgtc caaagcccac gacaatgacc tggggtttat
     8701 catcgttgac ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc
     8761 atttatccac cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca
     8821 gcaacagcgc catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga
     8881 caaaggcaaa ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac
     8941 gcacgccata caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa
     9001 ccacgcttat cactacccac aacagatggg tataaagcac cccgaggttg agtgacatcc
     9061 cgacagagat aaagaacaaa ccgagcagca agcctttaaa gggatcgata gccgtttcca
     9121 gttcatggcg atattcactt tccgccagca gcacgcctgc aataaacgta ccgagcgcca
     9181 tcgacagccc caacgcatcc ataaacaatg cggaacccaa taccagcagc agcgtcgcgg
     9241 cggtgaacac ttcccgcacg ccagaagctg caataaaacg gaataccgga cgcaataaat
     9301 agcgcccgcc aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt
     9361 cgaaatgttc gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg
     9421 ccagatcctg aaacagcagt accgaaaacc cgagctgccc ggattcgctg cgattcatcc
     9481 ctttctcacg catcaattgc aacgccattg cggtcgaaga catcgcaagg ccaatgccac
     9541 cgaccaccgc cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg
     9601 cgcttaacag cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct
     9661 tggagggatt caactcaagg ccgatgataa acatcaggaa caccacgccg agttccgaaa
     9721 agtggaggat ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc
     9781 ctgccagcaa atatcccaac acagcgccaa tacccagccg cgatgccaac ggcaccgcag
     9841 ccaccgccgc gaagagaaac agcactcctg caagtaaaaa atcggaacct tccatcagcg
     9901 gcctcctgga gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag
     9961 ctcctgtgcg ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg
    10021 gcacatgccc gccgccagtt caaaggggcg caggacatcg ctcatcgggt aacgattcaa
    10081 cgcgtcataa cggtaagcac tttccggctc gccggtggta atcacactac gccagtactt
    10141 tcccgccagt tggtttcctc ccggcccgct ggcaaaacca cggcttaata cccggtccag
    10201 ccactctttc agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac
    10261 ctcgtgctcg cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg
    10321 cgcgtaaagg tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg
    10381 gtttgccacc gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg
    10441 agacatcatc cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg
    10501 cgcccggtgc ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct
    10561 gaacatacgg caccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt
    10621 cctgctggat aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa
    10681 aaacggctgt ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg
    10741 cggcagctac acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccggc
    10801 gttaccgcaa gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga
    10861 agcgcagcta cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg
    10921 caagctggat gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg
    10981 cctcggtttc agcaatgaac aactggagcg cccggtaagt gatttctctg gtggctggcg
    11041 tatgcgtctt aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga
    11101 accgactaac cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta
    11161 tcagggcacg ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgtcgataa
    11221 aattattcat atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga
    11281 agtacagcgc gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg
    11341 cgtagcgcat ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca
    11401 ggcccagagc cgcattaaga tgctcgagcg tatggagctg attgcccccg cgcacgtcga
    11461 caacccgttc cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat
    11521 ggaaaaggtc agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct
    11581 ggtccccggt tcgcgcattg gtctgttagg ccgcaacggc gcgggtaaat cgacattaat
    11641 caaactgtta gccggtgaac ttgcgccagt cagtggtgag ataggcctgg caaaagggat
    11701 caagctcggc tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcgcctat
    11761 tcaacatctg gcacgtttag cgccgcagga actggagcaa aaactgcgtg actacctcgg
    11821 cggctttggt ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga
    11881 aaaagcccgc ctggtgctgg cattaatcgt ctggcagcgt ccgaatctgc tgctgctcga
    11941 cgaaccgact aaccaccttg accttgatat gcgtcaggca ctcaccgaag cattaatcga
    12001 gtttgaaggc gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga
    12061 cgatctctac ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc tggaagatta
    12121 tcaacagtgg ttgagcgacg tacaaaagca ggaaaaccag accgacgaag cgccaaaaga
    12181 gaacgcgaac agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agctgcgtgc
    12241 gcaaacccag ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa
    12301 cgcgcaactg gcgcaggcag aagagaaact cggcgacagc gaactgtatg accagagccg
    12361 taaagcggag ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga
    12421 gtgcgaaatg gcatggctgg aagcccagga gcagcttgag cagatgctgc tggaaggcca
    12481 aagcaactga tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa
    12541 ttcaccccta tgcgcggctt tagcaattgt catctgcaaa ccatgctgcc gcgtctgttt
    12601 cgtcgcaagg tgaaattcac cccgtactgg cagcgactgg agttgcccga cggcgatttt
    12661 gtcgatctcg cgtggagtga agcccctgca caggcgcaac ataaacctcg tctggtggtg
    12721 tttcacgggc tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg
    12781 caaaagcgcg gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac
    12841 cgtatgcacc gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg
    12901 ctgcaacgcg agtttggcca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat
    12961 atgctggcct gtttgctggc aaaagaaggc aatgatctcc cggttgatgc ggcggttatt
    13021 gtctctgcgc cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc
    13081 gtttatcagc gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc
    13141 taccccggaa cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa
    13201 tttgacgatc tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt
    13261 cagtgtagcg ccatgccgat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc
    13321 aaagacgatc cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag
    13381 gtggagtatc aactgactga acatggcggt catgttggct ttattggcgg tacattactt
    13441 catccgcaaa tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg
    13501 aaatcatgtt gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa
    13561 gctttgtgtt acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag
    13621 tcgccgacgt caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc
    13681 acgaaacggt caatatcatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg
    13741 gacgagataa taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat
    13801 tgcggtaaca ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat
    13861 attcgcgcag ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttacac
    13921 ccgcccggaa atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta
    13981 cttcggcccc gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca
    14041 gagcggcaaa gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa
    14101 tcaggtaccg ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt
    14161 ttatgaaggt ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga
    14221 cttactggtc ggcgtggtac ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga
    14281 caccagcgag cgcgggcact cacgagaagc agtgatggat tcagtagtgc gttcaatgga
    14341 agactatatc aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt
    14401 tcccaccgtc gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag
    14461 ctttgtggtg atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat
    14521 gttgcaaggc tcattcattt cccacatcaa tacgttagtg gtgcccggcg gcaaaatggg
    14581 tctggcaatt gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat
    14641 cgagtaactc tgctattacg ccggataaaa tactatccgg cttcacaacg ggatagttaa
    14701 atcacgcggc aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg
    14761 ccactgaaca atatttctca gcagagagat caaccgcacg cgcaaccgct gcgtctttca
    14821 ggtcgcgacc ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt
    14881 cgcggcgttc agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca
    14941 ggatcgaaac cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg
    15001 gacttggtgc tttatcgcct gagttgccgt ccattaaaat ctggtgacca gaggcagatt
    15061 cgcccagaaa agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac
    15121 tgcgtcaatt ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct
    15181 gggtcatgct gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag
    15241 gatgctacag taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca
    15301 gtacagttga tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc
    15361 ggcgttatct ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca
    15421 aaccgcaaac agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc
    15481 catccaagag cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta
    15541 aaggctctgt ggctgtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc
    15601 tgaatcaggg tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg
    15661 catgggtacg tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc
    15721 aattgattca ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc
    15781 tgcaagtcac ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg
    15841 cacagactct gctcaatctg gcaaaacaac cagacgctat gactcacccg gacggtatgc
    15901 aaatcaaaat tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg
    15961 gacgcattct gaagatgctt gaagatcaga acctgatctc cgcacacggt aaaaccatcg
    16021 tcgtttacgg cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt
    16081 gtttcccccg atgtggcgca gactgattta tcaccccgat attaactatg cactgcgaca
    16141 aacgctggtg ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg
    16201 tctgctcttc tctctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa
    16261 acgttttttc aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac
    16321 acagctgcta ctggcaaaag atgttcccct gccttttttg ctgaccggat taacgctggt
    16381 acttggcgtc actgctgagt tggggccatt gcacgcaaaa ttgcttcccg catcgctgct
    16441 ggcagccatt tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat
    16501 ctatgcgttg ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg
    16561 cgaacaaccg ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga
    16621 agccaaatac agcctgctta cccagcacac cgaccctgaa aaagcgctgc cgccgctgct
    16681 ggtgcgccag caaaaagcgg tcgatctaat tacccagtgc tatcagcaaa tgcatatgct
    16741 ttccgcgcaa aataatactg actacaagcg gatgctgcgt attttccagg aggcgctgga
    16801 tttacaggaa catatttcgg tcagtttgca tcagccggaa gaggtgcaaa agctggtcga
    16861 gcgtagccat gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg
    16921 cgtgctggct gatgacattc tttaccatcg cctgccgacg cgttttacga tggaaaagca
    16981 aattggcgca ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg
    17041 ctattggcac ttcagccgca tcgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg
    17101 tgacttactg gccgataaac agcggcgaat gccgttactt ccggcgctga aaagttattt
    17161 gtcactaaag tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc
    17221 cagcttgatg ggcaccgcgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt
    17281 gctggtgacg caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg
    17341 aaccgtggtc gggttaatca ttgcgggcgt ggcgctgcac tttaaaattc ccgaaggtta
    17401 caccctgacc ttgatgctga ttaccaccct tgccagctac ctgatattgc gcaaaaacta
    17461 cggctgggcg acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct
    17521 gaacggtgag cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc
    17581 tttcggtggt accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc
    17641 ccatgacgct ttagaagcct atcaggaagc gattcgcttg attctgagcg aggatccgca
    17701 accaacgcca ctggcctggc agcgaatgcg ggtaaatcag gcgcataaca ctctgtataa
    17761 ctcattgaat caggcgatgc aggaaccagc gttcaacagc cattatctgg cagatatgaa
    17821 actgtgggta acgcacagtc agtttattgt tgaacatatt aatgccatga ccacgctggc
    17881 gcgggaacac cgggcattgc cacctgaact ggcacaagag tatttacagt cttgtgaaat
    17941 cgccattcag cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt ctggcgatgc
    18001 caatatcatg gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca
    18061 gcatttacag cgggttattg gtcatctgaa caccatgcac accatttcgt cgatggcatg
    18121 gcgtcagcga ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaaggcgta
    18181 atgcaggcta aaccatcgga taaggcatgc acgccgcatc cgacatcttt tgcctgatgc
    18241 gtcttattta cgccccaacc accttcgcca ccgcgtgggc gaaacgttgc atcccttcat
    18301 cgatatccgc atcttccacc accagcgacg gcgcaaaacg catcacatcc ggtccggcat
    18361 tcagcaccat tacgccagcc tctgcgcccg catacaggaa atcacgcgcc tgacctttgt
    18421 actgtggttt cagctccgcg ccaattaaca gccccatacc gcgaatatcg ctaaacacat
    18481 cgtactgctg atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc
    18541 cttccagcac ttcaggggta ttaatgatat caaacgccgc gcccgctact gcacaggcca
    18601 gaggattacc gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg
    18661 ccgtggtcag catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa
    18721 tatccggcgt cacgccgtag tgcatgtaag caaacaaatc gccagtccgc cccatcccgc
    18781 actgtacttc atcaaacacc aataatgcct gatgttgatc gcacaactca cgcaagccct
    18841 gcaaaaactc tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca
    18901 ccgcacaggt gtgatcatcc atcaccgctt tcactgcgtg gagatcgtta aagggaacgt
    18961 ggatgatgtc tgatggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg
    19021 aaacggtaaa cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga
    19081 acgggctatg acgcacgcag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt
    19141 ccgtgccgga gttcataaac accacgcgtt cggcaaacgt cgcctcaatc agtttacgcc
    19201 caagacgcag cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct
    19261 gggtttttaa cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc
    19321 cacccgcgaa atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt
    19381 ttaccggaat gaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc
    19441 gtgtaattgc tgtttgttca
//
LOCUS       DBFIFS010000067        19404 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.067, whole genome shotgun sequence.
ACCESSION   DBFIFS010000067 DBFIFS010000000
VERSION     DBFIFS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.067"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            286..669
                     /gene="secE"
                     /locus_tag="N7A07_002194"
     CDS             286..669
                     /gene="secE"
                     /locus_tag="N7A07_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU2476853.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            671..1216
                     /gene="nusG"
                     /locus_tag="N7A07_002195"
     CDS             671..1216
                     /gene="nusG"
                     /locus_tag="N7A07_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU2476854.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1375..1803
                     /gene="rplK"
                     /locus_tag="N7A07_002196"
     CDS             1375..1803
                     /gene="rplK"
                     /locus_tag="N7A07_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU2476855.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1807..2511
                     /gene="rplA"
                     /locus_tag="N7A07_002197"
     CDS             1807..2511
                     /gene="rplA"
                     /locus_tag="N7A07_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU2476856.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2803..3300
                     /gene="rplJ"
                     /locus_tag="N7A07_002198"
     CDS             2803..3300
                     /gene="rplJ"
                     /locus_tag="N7A07_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU2476857.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3367..3732
                     /gene="rplL"
                     /locus_tag="N7A07_002199"
     CDS             3367..3732
                     /gene="rplL"
                     /locus_tag="N7A07_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU2476858.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4052..8080
                     /gene="rpoB"
                     /locus_tag="N7A07_002200"
     CDS             4052..8080
                     /gene="rpoB"
                     /locus_tag="N7A07_002200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU2476859.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8157..12380
                     /gene="rpoC"
                     /locus_tag="N7A07_002201"
     CDS             8157..12380
                     /gene="rpoC"
                     /locus_tag="N7A07_002201"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU2476860.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12593..13036
                     /locus_tag="N7A07_002202"
     CDS             12593..13036
                     /locus_tag="N7A07_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HGU2476861.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13471..14604)
                     /gene="thiH"
                     /locus_tag="N7A07_002203"
     CDS             complement(13471..14604)
                     /gene="thiH"
                     /locus_tag="N7A07_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU2476862.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14601..15371)
                     /gene="thiG"
                     /locus_tag="N7A07_002204"
     CDS             complement(14601..15371)
                     /gene="thiG"
                     /locus_tag="N7A07_002204"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU2476863.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15373..15573)
                     /gene="thiS"
                     /locus_tag="N7A07_002205"
     CDS             complement(15373..15573)
                     /gene="thiS"
                     /locus_tag="N7A07_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU2476864.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15557..16312)
                     /gene="thiF"
                     /locus_tag="N7A07_002206"
     CDS             complement(15557..16312)
                     /gene="thiF"
                     /locus_tag="N7A07_002206"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU2476865.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16305..16940)
                     /gene="thiE"
                     /locus_tag="N7A07_002207"
     CDS             complement(16305..16940)
                     /gene="thiE"
                     /locus_tag="N7A07_002207"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU2476866.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16940..18835)
                     /gene="thiC"
                     /locus_tag="N7A07_002208"
     CDS             complement(16940..18835)
                     /gene="thiC"
                     /locus_tag="N7A07_002208"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU2476867.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18916..19016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19068..>19404)
                     /gene="rsd"
                     /locus_tag="N7A07_002209"
     CDS             complement(19068..>19404)
                     /gene="rsd"
                     /locus_tag="N7A07_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU2476868.1"
                     /translation="RLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAK
                     IWPQLEANTQQIMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVL
                     DAARVKHPA"
BASE COUNT         4649 a         5003 c         5233 g         4519 t
ORIGIN      
        1 tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg agctaattgc
       61 cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt tttgcacgct
      121 ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc tgagacaggc
      181 tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt tgcctcgcga
      241 tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag tgcgaatacc
      301 gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt ggtggcattg
      361 ctcttggtgg cgattgtcgg caactatctt tatcgcgaca ttatgctgcc gctgcgtgcg
      421 ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac gacaaaaggt
      481 aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt catttggccg
      541 actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc agtaatgtca
      601 ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat cactggcctg
      661 aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg cgttttccgg
      721 ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca acatggaaga
      781 tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg gcggtcagcg
      841 tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg tgatgaacga
      901 cgcgagctgg cacctggtgc gcagcgtacc gcgtgtgatg ggcttcatcg gcggtacttc
      961 cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc gcctgcagca
     1021 ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg tccgtgttaa
     1081 tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg agaaatctcg
     1141 tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg acttcagcca
     1201 ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg cacaaggcgc
     1261 gagattggaa tacaatttcg cgccttttgt ttttatgggt cttgcccgta aaacgatttt
     1321 ttatatcacg gggagcctct aagaggcgtt attacccaac ttgaggaatt tataatggct
     1381 aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa cccgagtccg
     1441 ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg caaagcgttc
     1501 aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat caccgtttac
     1561 gctgaccgtt ctttcacttt cgttaccaag actccgccgg cagcagttct gctgaaaaaa
     1621 gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg taaaatttcc
     1681 cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg tgccgacatt
     1741 gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt agtggaggac
     1801 taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt tgatgcaacc
     1861 aaacagtacg acatcaacga agctatcgct ctgctgaaag agctggcgac tgctaaattc
     1921 gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc tgaccagaac
     1981 gtacgtggtg caactgtact gccgcacggt actggccggt ccgttcgcgt agccgtattt
     2041 acccagggtg caaacgctga agctgctaaa gctgcaggtg ctgaactggt aggtatggaa
     2101 gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat tgcttctccg
     2161 gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg cctgatgcca
     2221 aacccgaaag tgggtactgt aacgccgaac gttgctgaag cggttaaaaa cgctaaagct
     2281 ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat cggtaaagtg
     2341 gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc gctgaaaaaa
     2401 gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc caccaccatg
     2461 ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta atgcctttac
     2521 gtgggcggtg attttgtcta caatcttacc cccacgtatg ctaacgaaag ttagcagcta
     2581 aaaagatttg ttcgttggag cctggcctat ccaggcctcc gtcgaagacc gcaggagttt
     2641 cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat tcttttatat
     2701 tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg agtgaagtga
     2761 gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt aaatcttcaa
     2821 gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct gtctgcagta
     2881 gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa agcaggtcgc
     2941 gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc tgttgaaggt
     3001 actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc atactctatg
     3061 gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa tgcaaaattt
     3121 gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat cgaccgcctg
     3181 gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat gaaagaagct
     3241 tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga agctgcttaa
     3301 tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata ttcaggaaca
     3361 atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat gtctgtaatg
     3421 gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc tgctgctgct
     3481 gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt cgacgtaatt
     3541 ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg cgcaactggc
     3601 ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct gaaagaaggc
     3661 gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg cgctgaagtt
     3721 gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga tggctggtga
     3781 ctttttagtc accagccttt ttgcgctgta aggcaccagt agcgtttcac actgtttgac
     3841 tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc gacaggacgt
     3901 ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc attgcggaaa
     3961 gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca atggacagat
     4021 gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg agaaaaaacg
     4081 tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc tcctttctat
     4141 ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt atggtctgga
     4201 agctgctttc cgttccgtat tcccgattca gagctacagc ggtaattccg agctgcaata
     4261 cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa tccgtggcgt
     4321 gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc gcgaagcgcc
     4381 ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa ttccgctcat
     4441 gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt cccagctgca
     4501 ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt cgggtaaagt
     4561 gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg aattcgatcc
     4621 gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga ccatcattct
     4681 gcgtgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa aagttatctt
     4741 tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc gtggtgaaac
     4801 cgcatccttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc gccgtatcac
     4861 tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag tcccggttga
     4921 gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg gcgagctgat
     4981 ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc agtctggtca
     5041 caagcgtatc gaaacgctgt tcaccaatga tctggatcac ggcccgtata tctctgaaac
     5101 cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct accgcatgat
     5161 gcgccctggc gagccgccga ctcgtgaagc agcggaaagc ctgttcgaga acctgttctt
     5221 ctccgaagac cgttatgacc tgtctgcggt tggtcgtatg aagttcaacc gttctctgct
     5281 gcgcgaagaa atcgaaggtt ctggtatcct gagcaaagac gacatcattg atgttatgaa
     5341 aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc acctcggcaa
     5401 ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg gcctggtacg
     5461 tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc tgatgcctca
     5521 ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg gttccagcca
     5581 gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca aacgtcgtat
     5641 ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag ttcgagacgt
     5701 acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc cgaacatcgg
     5761 tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc ttgagactcc
     5821 gtatcgtaaa gtgactgacg gtgttgtaac tgacgaaatt cactacctgt ctgctatcga
     5881 agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag gccacttcgt
     5941 agaagacctg gtaacctgcc gtagcaaagg cgaatccagc ttgttcagcc gtgaccaggt
     6001 tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc tgatcccgtt
     6061 cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac gtcaggccgt
     6121 tccgactctg cgtgctgata agccgctggt tggtactggt atggaacgtg ctgttgccgt
     6181 tgactccggt gtaactgcgg ttgctaaacg tggtggtgtc gttcagtacg tggatgcttc
     6241 ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag gtatcgacat
     6301 ctacaacctg accaaataca cccgttctaa ccagaacacc tgtattaacc agatgccgtg
     6361 tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc cgtccaccga
     6421 ccttggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt ggaatggtta
     6481 caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc gtttcaccac
     6541 catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc cagaagagat
     6601 caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactggatg aatccggtat
     6661 cgtttatatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg ttacgccgaa
     6721 aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg gtgagaaagc
     6781 gtctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta cggttatcga
     6841 cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg aaatcgaaga
     6901 aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc tcgaagcggg
     6961 tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg agaagctcga
     7021 caaattgccg cgcgatcgct ggctggagct gggcctgacc gacgaagaga aacaaaatca
     7081 gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga agaaactcga
     7141 agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc tgaagattgt
     7201 taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg caggtcgtca
     7261 cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt acgatgaaaa
     7321 cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta tgaacatcgg
     7381 tcagatcctc gaaacccact tgggtatggc tgcgaaaggt atcggcgaca agatcaacgc
     7441 catgctgaaa cagcagcagg aagtcgcgaa actgcgtgaa ttcatccagc gtgcgtacga
     7501 tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg aagaagttat
     7561 gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt tcgacggtgc
     7621 gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt ctggtcagat
     7681 ccgcctgtac gacggccgca ctggtgaaca gttcgaacgt ccggtaaccg ttggttacat
     7741 gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt ccaccggttc
     7801 ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg gtcagcgttt
     7861 cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc tgcaggaaat
     7921 gctcaccgtt aagtctgatg acgtgaacgg tcgtactaag atgtataaaa acatcgtgga
     7981 cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt tgaaagagat
     8041 tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca aacaggtcac
     8101 tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc cgacgggagc aaatccgtga
     8161 aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat gcgatcaaaa
     8221 ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt aaaaagccgg
     8281 aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc gcccgtatct
     8341 ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg aaacaccgtg
     8401 gcgtcatctg tgagaagtgt ggcgttgaag tgacccagac taaagtacgc cgtgagcgta
     8461 tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa tcgctgccgt
     8521 cccgtatcgg cctgctgctc gatatgccgc tgcgcgatat cgaacgcgta ctgtactttg
     8581 aatcctatgt ggttatcgaa ggcggtatga ccaacctgga gcgtcagcag atcctgactg
     8641 aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg aaaatgggtg
     8701 cggaagcaat ccaggccctg ctgaagagca tggatctgga gcaagagtgc gaacagctgc
     8761 gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc aagcgtatca
     8821 aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc ctgaccgttc
     8881 tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt cgtttcgcga
     8941 cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt ctgaaacgtc
     9001 tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg ctgcaggaag
     9061 cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt tctaacaagc
     9121 gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc cgtcagaacc
     9181 tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtgggt ccatacctgc
     9241 gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa ccgttcatct
     9301 acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag aaaatggttg
     9361 agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa cacccggtac
     9421 tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa ccggtactga
     9481 tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac gccgacttcg
     9541 atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg gaagcgcgtg
     9601 cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca atcatcgttc
     9661 cgtctcagga cgttgtactg ggtctgtact acatgacccg tgactgtgtt aacgccaaag
     9721 gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc tctggtctgg
     9781 cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat gctaacggtg
     9841 aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt ctgtggatga
     9901 ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa aaagcaatct
     9961 ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt atttttgcgg
    10021 accagatcat gtacaccggc tttgcatatg cagcgcgttc tggtgcatct gttggtatcg
    10081 atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa gcagaagttg
    10141 ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc tacaacaaag
    10201 ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg gataacctgc
    10261 aaactgaaac cgttattaac cgtgacggtc aggaagagaa gcaggtttcc ttcaacagca
    10321 tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt cagcttgctg
    10381 gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca atcaccgcga
    10441 acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt gctcgtaaag
    10501 gtctggcgga taccgcactg aaaactgcga actccggtta cctgactcgt cgtctggttg
    10561 acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa ggtatcatga
    10621 tgactccggt tatcgagggt ggtgacgtta aagagccgct gcgcgatcgc gtattgggtc
    10681 gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt ccgcgcaaca
    10741 cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac gcggttaaag
    10801 tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc tacggtcgtg
    10861 acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc gcggcacagt
    10921 ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt ggtgcggcat
    10981 ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc aagctcagca
    11041 acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt aacaccgaac
    11101 tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct tacggtgcgg
    11161 tactggcaaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca aactgggacc
    11221 cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact gacatgatcg
    11281 acggccagac cattactcgt cagaccgacg aactgaccgg tctgtcttcg ctggtggttc
    11341 tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg aaaatcgttg
    11401 atgctcaggg taacgacgtt ctgatcccag gtaccgatat gcctgcgcag tacttcctgc
    11461 cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt gacaccctgg
    11521 cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg ccacgcgttg
    11581 cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa atcagcggta
    11641 tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct ggttatcacc ccggtagacg
    11701 gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg ttcgaaggtg
    11761 aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac gacattctgc
    11821 gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa cgaagtacag gacgtatacc
    11881 gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt cagatgctgc
    11941 gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa caggttgaat
    12001 actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg ggtgcaactt
    12061 actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc ttcatctccg
    12121 cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg ggcaaacgcg
    12181 acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg gcaggtaccg
    12241 gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct ccggctgcac
    12301 cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac gcaggtctgg
    12361 gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg
    12421 tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag gaatttctga
    12481 atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact aattgagtca
    12541 aactcggcaa ggattcgata ctattcctgt gtagctttct taaggaacga gaatgaaaca
    12601 ggaagtggag aagtggcgac cttttaaaca tccggatggc gacgttcgtg atttatcgtt
    12661 tcttgatgct catcaggtta tctacgttca gcatcatgag gacaaggagc ctttagagta
    12721 tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac atcagacgaa
    12781 cgaagaaaaa ctatcgttaa tgtaccacgc gcctaaagaa tctcgtccct tctgccagca
    12841 ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc cagaaagcaa
    12901 cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag acggaggaga
    12961 taaggcattt tactttgttg tgttcagggc tttcagtgaa atttttcacc attgcctaaa
    13021 acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca ccttaaggtg
    13081 ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt tgccttgacc
    13141 ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca tcctggaaga
    13201 tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc tcttacaatc
    13261 agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg aatttttaag
    13321 aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct gctgacggca
    13381 ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc cgtagcggct
    13441 cggaataaat tttcgttctt acattccgtc tcatagtctt tgcgaggcgc gtcccagata
    13501 gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg cagcaaccgc
    13561 ttccggtctg cgctcgtcgt gcggtgagaa ctgttccagc tcggggtgat tgtcggcata
    13621 gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg gaataacgcg
    13681 atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg gtgcaagcag
    13741 ccggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg caggctcaat
    13801 gccgccagta catggacgca ggcgtgggaa ggagacagag tagcggcttt gccagtaatg
    13861 ctgttgcagc catagcaaat gttctgcaac catatagcag tcaacgcgcc agctgtcgga
    13921 aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacggccca gccgatccgg
    13981 cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc gggcataagt
    14041 cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagtt gctttaactc
    14101 ggcgtattcc gtctccgcca gcggctgtac ttccatctgt agtgaagaga actgttcacg
    14161 cagggcaggg agatgacgac gaaagtaatc catccccact ttcgcctgat gttcaccggt
    14221 gactaaaagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc tggcaatatc
    14281 cgcttcatcc agcgttttgc gcttgatgcg gttgctcata gagaatccgc agtacgtgca
    14341 gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg tgttgccaaa
    14401 tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc cactggcggc
    14461 tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta gcgcccgctc
    14521 tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt ccagttgtcg
    14581 ccagcgatcg ctgaaggttt tcataccgat gcctccagaa atccggtcag cgggctggtg
    14641 gcatgagcaa aataactgcg gctgcccggt ccggactgag gtgccagcag gcctgcttct
    14701 actgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc aatcgccgta
    14761 ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct gggaacgccg
    14821 atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc cagcatggcg
    14881 cgggtttcca gcccctgatt cgagccaatc ggcgcgccga gcggcatcac cgctgcacag
    14941 ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg cagcacgaca
    15001 aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc gggcaacagc
    15061 cagcgggcgt cagggtgaat ctccaatttt aaccagtttg tgcctaacgc ttcacgagcc
    15121 agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt tggcagcagg
    15181 gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg caagtcgaca
    15241 cgtttcatcg ccagcgtcac cagctggctg ccggaagcgc ggattgcctc taccatcagt
    15301 tgtgaagagg cgaatttccc ggtgccggta aacagatgtg aatcaaacgt tttgtccgca
    15361 atacgtaaca tctcaacccc ctgcaataac ctgaaaaagt aggatctggt cgccatcctg
    15421 cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg ccagagccgc
    15481 gcccgcttgt cgttgaacga gttgctccag tagttcgtga acagtttgcc cggcggcgca
    15541 ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc gggcaaccac
    15601 tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac agtcggagtt
    15661 ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc tgcaaagtgc
    15721 ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt tctggctcct
    15781 ggttttctgg ccacaggcag cggtaacaac cctgccccca gggcggcgtc agtaccatca
    15841 actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt gccacgcagg
    15901 cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc acatcggctt
    15961 gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct gtcagttgaa
    16021 tatcgggatt caactgtgtc agtcgctgat ggctgacctg cgatttcggg cgatcgatat
    16081 cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg tcgtcatctg
    16141 ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt gtacccagcc
    16201 cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg tcaagagcga
    16261 tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc atcgccaact
    16321 cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg agtaatggcg
    16381 ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg tgccagactg
    16441 ataccgccaa tcgccacggt gggataatcc gacagtcgct caacatgccg tgccagctgt
    16501 tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac atgtcccagc
    16561 gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc atgggtcgaa
    16621 acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg caaatcttcc
    16681 tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta atcgttgata
    16741 aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc ggcttccacc
    16801 tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc atccaacaga
    16861 cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa aggtacagga
    16921 ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct ccgcctctgg
    16981 cacggaagtt ctccgacata tccgctattc ccatttcaat agtttgcgtg gcggcgtaat
    17041 cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg gagcaaaaat
    17101 gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg gcggtaaacg
    17161 ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct ttcgacatgg
    17221 cgttatcgcg aatttgtgcg cccggatgcc ctttcgccag gtcagcggcg tgggcggcaa
    17281 tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc agatgctctt
    17341 ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc gcaccaatcc
    17401 ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc agagtgtaaa
    17461 acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc atctgcatcg
    17521 gcacgtggcc tgggccttca atcatcacct gcacatcata ttcccaggcg atttttgtca
    17581 gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc tgaatagaac
    17641 cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag atttcacaaa
    17701 tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac catttcgcca
    17761 taatcgaacc gccgcgcgag acgataccgg tcaggcgttt cgcggtcatc ggcacatagc
    17821 gcagcagcac ccccgcatgg atagtgaagt aatccacacc ttgctcggcc tgttccagca
    17881 gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccattaacc ttctccagcg
    17941 cctggtagat cggcactgta ccaatcggca ccgggctgtt acgcaaaatc cactcgcggg
    18001 tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc cagcgcgtgg
    18061 accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag ttgccgatat
    18121 tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat tccggatgat
    18181 taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca aattccgcag
    18241 tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg cgtaaaactt
    18301 cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc atttccggtg
    18361 tgatgatgcc ctggcgggcg tagtgcagtt gtgtgacgcg gcgtcctgct ttggcgcgtt
    18421 ttggcgttaa tacgccgcta aaacgcagct cgtcgaggcc atcatctgcc agccgcgctt
    18481 tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca tcgatccacg
    18541 gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga tcgccatacg
    18601 ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc tgttctttgc
    18661 taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc acgccgggtt
    18721 gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc agggtgtcga
    18781 taaaatgttg ggcctgggcg cgttgttcgc gacgggtcag ttttgttgca gacatagctc
    18841 attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag acgatcgccg
    18901 caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag cctgatcagg
    18961 ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa gaaattagcc
    19021 ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca agcaggatgt
    19081 ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac aaagcgcgct
    19141 tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt atcatgatcg
    19201 atagcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt ggcttcgagt
    19261 tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt atgaagaatg
    19321 cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg acaaaaatca
    19381 tcaagggctt tttcgtttag cctc
//
LOCUS       DBFIFS010000068        19117 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.068, whole genome shotgun sequence.
ACCESSION   DBFIFS010000068 DBFIFS010000000
VERSION     DBFIFS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.068"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(173..1822)
                     /gene="pgi"
                     /locus_tag="N7A07_002210"
     CDS             complement(173..1822)
                     /gene="pgi"
                     /locus_tag="N7A07_002210"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU2476869.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2059..2255
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2347..3696
                     /gene="lysC"
                     /locus_tag="N7A07_002211"
     CDS             2347..3696
                     /gene="lysC"
                     /locus_tag="N7A07_002211"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU2476870.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3949..4221
                     /locus_tag="N7A07_002212"
     CDS             3949..4221
                     /locus_tag="N7A07_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU2476871.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4222..5094)
                     /gene="rluF"
                     /locus_tag="N7A07_002213"
     CDS             complement(4222..5094)
                     /gene="rluF"
                     /locus_tag="N7A07_002213"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU2476872.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5442..6389
                     /locus_tag="N7A07_002214"
     CDS             5442..6389
                     /locus_tag="N7A07_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU2476873.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6459..7265
                     /locus_tag="N7A07_002215"
     CDS             6459..7265
                     /locus_tag="N7A07_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2476874.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7275..7682
                     /locus_tag="N7A07_002216"
     CDS             7275..7682
                     /locus_tag="N7A07_002216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU2476875.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7682..8176
                     /locus_tag="N7A07_002217"
     CDS             7682..8176
                     /locus_tag="N7A07_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU2476876.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8242..9039
                     /locus_tag="N7A07_002218"
     CDS             8242..9039
                     /locus_tag="N7A07_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU2476877.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9050..9874
                     /locus_tag="N7A07_002219"
     CDS             9050..9874
                     /locus_tag="N7A07_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU2476878.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9926..11155
                     /gene="sorE"
                     /locus_tag="N7A07_002220"
     CDS             9926..11155
                     /gene="sorE"
                     /locus_tag="N7A07_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU2476879.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11538..12227
                     /gene="pepE"
                     /locus_tag="N7A07_002221"
     CDS             11538..12227
                     /gene="pepE"
                     /locus_tag="N7A07_002221"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU2476880.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12318..13949)
                     /locus_tag="N7A07_002222"
     CDS             complement(12318..13949)
                     /locus_tag="N7A07_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU2476881.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14169..17852)
                     /gene="metH"
                     /locus_tag="N7A07_002223"
     CDS             complement(14169..17852)
                     /gene="metH"
                     /locus_tag="N7A07_002223"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU2476882.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18052..18876
                     /gene="iclR"
                     /locus_tag="N7A07_002224"
     CDS             18052..18876
                     /gene="iclR"
                     /locus_tag="N7A07_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU2476883.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4504 a         4978 c         5025 g         4610 t
ORIGIN      
        1 ttaaggttat ttaattaatc gtgctatgca ttaattgccg aatacgatgt acatatcggc
       61 atcgacctgt aggcctgata agacgcgtaa agcgtcgcat caggcaccag ttgccggatg
      121 cggcgtaaac gccttatccg gcctacatag cggcatcagg catgaacgat gattaaccgc
      181 gccacgcttt atagcggtta atcagaccat tggtcgagct atcgtggctg ctgatttctt
      241 tatcatcttt cagctctggc agaatacggt tcgccagctg tttacccagt tccacgcccc
      301 actggtcgaa ggtgaagatg ttcaggatca cgccctgagt aaagattttg tgctcataca
      361 gcgcaatcaa cgcacccagg ctgaacggag tgatttcgcg caacaggata gagttggtcg
      421 ggcggttacc ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc ggatctttac
      481 cctgatcgcg atattcctgc tcaaccactt cgcgggattt accaaacgcc agcgcttcgg
      541 tctgggcgaa gaagttagac agcagtttct ggtggtgatc agagagcggg ttatgggtga
      601 tagccggagc gatgaaatcg cacggtacca ttttggttcc ctggtggatc agctggtaga
      661 acgcgtgctg accgttagtg cctggttcac cccagataat cgggccagtc tggtaatcca
      721 caacgttacc gttacggtca acatacttac cgttggactc catattgccc tgctggaagt
      781 acgccgcgaa acggtgcata tactggtcat acggcagaat cgcttcagtt tccgcaccaa
      841 agaaattgtt gtaccagatg ccaatcagcg ccaacagtac aggcaggttt ttctcggcag
      901 gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca acgaagttat
      961 caaagccgat ggagagaaca atcgacaggc caatcgctga ccacaaagag taacgaccgc
     1021 cgacccagtc ccagaactcg aacatgttgg cagtatcaat accaaactcg ccaacggctt
     1081 tggcattggt tgagagcgcc gcaaagtgtt tcgcaacgtg tttttcatca cctgccgctt
     1141 tcaggaacca gtcacgcgcg ctatgggcgt tggtcatggt ttcctgagtg gtgaaggttt
     1201 tagatgctac caggaacagc gtggtttccg ggtttacttt tttcagcact tcagcgatgt
     1261 gagtcccatc gacgttagaa acaaagtgca tgttcaggtg atttttgtac ggacgcagtg
     1321 cttcggtcac catgtacggg ccgaggtcag aaccgccgat cccgatgttc actacgtcag
     1381 tgattgcttt gccggtataa cccttccact caccggagat aatcgcttcc gagaaggttt
     1441 tcatcttctc cagcaccgcg ttgacttctg gcataacatc tttgccatca accagaatcg
     1501 gggtattgct acgattacgc agcgctacgt gcagcacggc gcggttttca gtgcggttga
     1561 tcttctcgcc agagaacatc gacttaatcg cgcccgccag atcgcactct ttcgccagat
     1621 cttgtaattt cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc accagcatct
     1681 gatcgtcgaa ggttgcggag aacttagaaa aacgatcgcc gtctttagca aaaagatcgg
     1741 cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag tgcctgccag gcagcggtct
     1801 gcgttggatt gatgtttttc attagcaata ctcttctgat tttgagaatt gtgactttgg
     1861 aagattgtag cgccagtcac agaaaaatgt gatggtttta gtgccgttag cgtaatgttg
     1921 agtgtaaacc cttagcgcag tgaagcattt attagctgaa ctactgaccg ccaggagtgg
     1981 atgaaaaatc cgcatgaccc cgtcgttgac aaccgccccg ctcacccttt atttataaat
     2041 gtactacctg cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg gtgttggagg
     2101 agccagtcct gtgataacac ctgagggggt gcatcgccga ggtgattgaa cggctggcca
     2161 cgttcatcat cggctacagg ggctgaatcc cctgggttgt caccagaagc gttcgcagtc
     2221 gggcgtttcg caagtggtgg agcacttctg ggtgaaaata gtagcgaagt atcgctctgc
     2281 gcccacccgt cttccgctct tcccttgtgc caaggctgaa aatggatccc ctgacacgag
     2341 gtagttatgt ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc tgattttgac
     2401 gccatgaacc gcagcgctga tattgtgctt tctgatgcca acgtgcgttt agttgtcctc
     2461 tcggcttctg ctggtatcac taatctgctg gtcgctttag cggaaggact ggaacctggc
     2521 gagcgattcg aaaaactcga cgctattcgc aacatccagt ttgccattct ggaacgtctg
     2581 cgttacccga acgttatccg tgaagagatt gaacgtctgc tggagaacat tactgttctg
     2641 gcagaagcgg cggcgctggc aacgtctccg gcgctgacag atgaactggt cagccacggc
     2701 gagctgatgt cgaccctgct gtttgtcgag atcctgcgcg aacgcgatgt tcaggcacag
     2761 tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc agagccagat
     2821 gtagccgcgc tggcggaact ggccgcgctg cagctgctcc cacgtctcaa tgaaggctta
     2881 gtgatcaccc agggatttat cggtagcgaa aataaaggtc gtacaacgac gcttggccgt
     2941 ggaggcagcg attatacggc agccttgctg gcggaggctt tacacgcatc tcgtgttgat
     3001 atctggaccg acgtcccggg catctacacc accgatccac gcgtagtttc cgcagcaaaa
     3061 cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg tgcaaaagta
     3121 ctgcatccgg caacgttgct acccgcagta cgcagcgata tcccagtctt tgtcggctcc
     3181 agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa tccgccgctg
     3241 ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca ctttgcacag cctgaatatg
     3301 ctgcattctc gcggtttcct cgcggaagtt ttcggcatcc tcgcgcggca taatatttcg
     3361 gtagacttaa tcaccacgtc agaagtgagc gtggcattaa cccttgatac caccggttca
     3421 acctccactg gcgatacgtt gctgacgcaa tctctgctga tggagctttc cgcactgtgc
     3481 cgggtggagg tggaagaagg tctggcgctg gtcgcgttga ttggcaatga cctgtcaaaa
     3541 gcctgcggcg ttggcaaaga ggtattcggc gtactggaac cgttcaacat tcgcatgatt
     3601 tgttacggcg catccagcca taacctgtgc ttcctggtgc ccggcgaaga tgccgagcag
     3661 gtggtgcaaa aactgcatag taatttgttt gagtaaatac tgtatggcct ggaagctata
     3721 tttcgggccg tattgatttt cttgtcacta tgctcatcaa taaacgagcc tgtactctgt
     3781 taaccagcgt ctttatcgga gaataattgc ctttaatttt tttatctgca tctctaatta
     3841 attatcgaaa gagataaata gttaagagaa ggcaaaatga atattatcag ttctgctcgc
     3901 aaacgaattc ccgtacactg aactctctgg ctcactgagg gcaccatcat ggcactcccc
     3961 cgtattaccc aaaaagagat gactgaacgc gagcagcgtg aacttaaaac actgctggat
     4021 cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg aaaccaacag cattaagaaa
     4081 gagtacatcg ataaattaat ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagtta
     4141 aagaaaaagc aggcttataa accggatcct gaagcgtcat ttagctggtc agcaaatacg
     4201 tcgacgcgcg gcaggcgctg attagcgccc cttctttttt cgccccggcg aggtaaaacg
     4261 cttaccgttg gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg
     4321 acgtttgatg cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga
     4381 attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc
     4441 cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc
     4501 atagccgaaa tgctcgcaca tgcgccggat ctgacggttc agcccctgca ccagggtaat
     4561 gcggaagaca aacggcgctt cttttttgac tttgcacttt ttagtcaccg tcccgaggat
     4621 cggcaccccc gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac
     4681 cagatactct ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg
     4741 attggtgagg aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg
     4801 tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac
     4861 aatgcctacg ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat
     4921 caactgaccg tttactttca caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg
     4981 cttgccatta aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca
     5041 aattccgctt tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc
     5101 tcctgtaaaa cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa
     5161 cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacaa
     5221 tgcaaatttg cgtggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg
     5281 ctgataacta ctgcgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca
     5341 aagagtgaaa aatcgctttg aaaggccatc atgatggggt tatatcagat caagcggtga
     5401 gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata
     5461 tccgtttgat tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa
     5521 tcgcgcgcga actgggtatt taccgcacca acatcagccg cttgcttaaa cgaggccgcg
     5581 atcagggaat tgtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc
     5641 agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg
     5701 atgaagagac tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc
     5761 tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa
     5821 acttgccgca ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt
     5881 ccggtaaact cgaaagccgc tatcacgtaa acacattaac ctacagcgcg gcagcgaagc
     5941 tgaaagggga atcgcatctc gcggattttc cggctctact ggataaccca ttaattcgta
     6001 atgggatcat gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggatg
     6061 tcgccctggt gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt
     6121 atggtggtga agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc
     6181 gcttttttga tattcacggc gaaatggttg aaacgaatat gagcgaaaaa acactctcta
     6241 tcgaaatgaa taaattaaag caggcacgat attccattgg cattgccatg agtgaagaaa
     6301 aatacagcgg aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata
     6361 gcagcacagc tgaactatta ctgaaataaa aatcttcccg atgtttatcg taataaaaat
     6421 tacggcgggt ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc
     6481 aggataaaat cattatcgtc accggcggcg catccggtat tggtctggcg attgtagagg
     6541 aattattagc acaaggcgca aacgtacaga tggtagatat tcacggtggc gatggacaat
     6601 atgaaggtca taaaggctat cagttttggc cgaccgatat ttccagcgcc aaagaggtaa
     6661 atcatacggt agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg
     6721 ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac
     6781 tcaacgaagc tgcattcgaa aaaatggtca atatcaacca gaaaggcgtt tttctgatgt
     6841 cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct
     6901 cagaaagtgg gctggaaggc tcagaaggcc aaagctgtta cgccgctacc aaagccgcgc
     6961 tcaatagctt cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg
     7021 gtatcgcgcc ggggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc
     7081 tggcgtggac gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca
     7141 ttcctattgg gcgcgccgga agattagcag aaatcgctga ttttgtttgt tatctgctgt
     7201 ctgaacgcgc cagctatatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg
     7261 gctaaggagg ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca
     7321 tgctggaatc ggttcagatg gtttacggca acgccaatgt tgaagccgtt gaatttgtgc
     7381 ccggtgaaaa cgccggagac atcgtagcaa agctggaaaa attagtaagc attcacaatc
     7441 aaaatgagtg gctgattgcc gtggatttac agtgcggcag cccgtggaat gcggcagcga
     7501 tgcttgcgat gcgcaatcct cgtctgcggg taatcagcgg actttctctg ccgctggcgc
     7561 tcgaactggt tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc
     7621 aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct
     7681 gatgaacatt acgctcgccc gcattgatga ccgcctgatc cacggtcagg tcactaccgt
     7741 ctggtcgaaa gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga
     7801 tgaagttcgc cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt
     7861 caatattgaa aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt
     7921 ttatcttttc actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg
     7981 caccctgaat attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt
     8041 ttcattagat gatgatgata ttaacgcatt tcatgagtta aataatctag gcgtcatact
     8101 ggatttacgc gtcgtagcgt cagatccctc aatcaatatt atctacaaaa taaacgaaca
     8161 gttaatcgca aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg
     8221 acacttaaaa ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt
     8281 ttttcctgta ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta
     8341 attgcctgta cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggt
     8401 ggtacgctgg aattgatagc tctcggctgg atgaacgtcg gcgcggcgca atctccggat
     8461 tctgcactcg ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc
     8521 accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc
     8581 cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag cagcggaaga agcacggttt
     8641 cgcactctcg atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt
     8701 ccggcactga ttgtctcgct gttcgtcagc gccgatatgg tgagcaatat gttaaacgcc
     8761 attcccgaat ttgtgacccg aggtctgcaa attgctggcg gttttatcgt ggtagtcggt
     8821 tacgccatgg tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt
     8881 ttcctcgcag gtggctacct cgatctcagt ctgctggcgt tcggtggcgt cggcgtgatc
     8941 atggccctgg tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactcctg
     9001 gcccccgctt ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag
     9061 aaaaattaca cgcggcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc
     9121 ctttaacttt gaacgtattc acgggctggg tttttgctac gacatgatcc ccgccatcaa
     9181 gcgactttac ccagtaaaag aggatcaggt tgcggcgctc aggcgacacc tggtgttctt
     9241 taataccacg ccagccgtat gtggcccggt catcggcgtc actgccgcca tggaagaggc
     9301 gcgggccaac ggcgcggaaa ttgatgacgg caccattaac ggcatcaaag tcggtctgat
     9361 gggaccattg gcaggggttg gcgatccact ggtctgggga acgctgcgcc cgattaccgc
     9421 cgcgctcggc gcatcgctgg cgctgtctgg caacattctc ggcccgctgc tgttcttctt
     9481 tattttcaac gcggtgcgtc tggcgatgaa gtggtatggc ctacagctcg gctttcgcaa
     9541 aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg caaaaactca ccgaaggcgc
     9601 gtcgattctc ggactgtttg tgatgggcgt gctggtcacc aaatggacgt caatcaacgt
     9661 accgttggtg gtttcacaaa cgcatgccgc cgatggctcc accgtcacca tgaccgtgca
     9721 gaacattctc gatcaacttt gccctggttt gctggcgctc ggtctgacgc tactgatggt
     9781 tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt ttcgccctgt ttggcttagg
     9841 gattatcggc aatgcgctgg gcttcctgtc ctgattcttc gccccggcac gactgccggg
     9901 gccatcgttc aacatgaggt ggtttatgaa aacaacagct ctgcgtcttt atggtaaacg
     9961 tgatttacgc ctggaaacct ttgaccttcc tgaaatgcag gaggatgaaa tcctcgcgac
    10021 ggtggtcact gacagcctgt gcctctcttc ctggaaagag gccaatctgg gtgaaaacca
    10081 taaaaaagta cccgacgatg tggcgaccaa cccaatcatc atcggccacg agttttgcgg
    10141 cgatattctg gccgtgggta aaaagtggca gcacaaattc cagccgggtc agcgttatgt
    10201 gattcaggcc aacctgcaac tccccgaccg cccggactgc cccggctact ccttcccgtg
    10261 ggtaggcggc gaggccacgc atgtggttat tcccaacgag gtcatggaac aagattgcct
    10321 gctggcatac gacggcgaaa cctattttga aggctcgctg gttgaaccgc tttcctgcgt
    10381 gattggcgcg ttcaacgcca actatcatct tcaggaaggt agttataacc acacgatggg
    10441 gattcgcccg caagggcgca tgctgatcct cggcggcacc ggaccaatgg gactgttggc
    10501 gattgattat gcgctacatg gacccgttaa cccgtcgctg ctcgtcatta ccgataccga
    10561 caacgataaa ttgagttatg cgcgcaagca ctatccatca gaaccgcaaa cactgattca
    10621 ttatctcaat gccgccgatg cagcatttga tacgctaatg gcgctgagtg gcggtcacgg
    10681 cttcgatgat attttcgtct ttgtgcctaa tgaaggactg gtgactctcg cctcttcctt
    10741 gctggcgaca gatggttgcc tgaatttctt cgccggaccg caggataaac atttcagcgc
    10801 gccaattaat ttctacgatg tgcattatgc atttacccac tacgtgggca cgtcaggcgg
    10861 caataccgac gacatgcgcg cagcggtcaa attgattgaa gagaaaaaag tgcaggccgc
    10921 aaaagtggta acacatattc ttgggctgaa tgccgcgggc gaaaccacgc ttgaattgcc
    10981 tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa tacctgccgc tgacgtcact
    11041 cacgcagatt caggatcaag cactggcggc gattctggcg cgtcatcagg ggatctggtc
    11101 cggtgaggcg gagcaatatc tgctcactca tgcagaggca atttcccatg attaatcgcg
    11161 ctacttaact ctgcatgtcg ctggctggtc accgcggcaa cttcgcgacc tgatgtgcgg
    11221 ctgattgctg aagcggccca atgtgcacga gaaggataaa aaaacatcag cgccttaagg
    11281 ggcgctcaaa ttggtggcaa agtacacatt gtttatgccg gatgcggaat aaagccttat
    11341 tcggcctaca taatctcgaa aattcaatat attgcagtta tctggtaggc ctggtaagcg
    11401 tagcgcatca ggcaatgctg tgtttgtcat cagtctcagc gcccggtgca atacgtgaca
    11461 tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta gcatgccttc cgtgatttct
    11521 tactaaccgg agaagtaatg gaactgcttt tattgagtaa ctcgacgctg ccgggtaaag
    11581 cctggctgga acatgcactg ccgctgattg ctgaacagtt gcagggtcgc cgctcagcgg
    11641 tgtttatccc tttcgctggc gtaacgcaga cctgggatga ttacacagcg aaaacggctg
    11701 cggttctcgc tccgctgggt gtttctgtca ccggtattca tagcgttgtc gatcccgttg
    11761 ccgcgattga aaatgcggaa attgtgattg tcggcggcgg taatactttc cagttgctga
    11821 aacagtgccg cgagcgcggg ctgctggctc caattactga cgtggttaaa cgtggcgcgc
    11881 tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aaccattcgt accaccaacg
    11941 atatgccgat tgtcgatccg caaggtttcg atgcgctaaa tctgttcccg ttgcaaatca
    12001 acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt gagcagcgta
    12061 ttcgcgaact gctggtcgtc gcgccagaac tgacgattat tggtctaccg gaaggtaact
    12121 ggatcacagt gagtaaaggt cacgcaacgc tgggtggccc gaataccacc tatgtgttta
    12181 aggccggtga agaagcggtt ccgctggaag ctggtcaccg tttttaagtg aattgccgga
    12241 tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg cttgacgcgt cttatcaggc
    12301 ctacaggatt cacaatatta atactcgtcc cgtccctcat cttcatcagg ctgttccagc
    12361 acactgtaag ccaccgaaca aaacagcgag ttcagacgct gcatatctcc cagtaagcct
    12421 aaatgtagcg aactggtttc aatgctttgc acgttttgct gatgcaggcg atcgacgtgg
    12481 gcgtgggaat agcggcgatt aagaatgcga aagcgatgct tgctgcggcg caaacgacga
    12541 gcgctggtga catcgccaga gaagaacacc gacattgcca gctttaaatt actgagcaat
    12601 tgctcataga gcgcatccag ttccttcaag ccatcaagcg aaaatgctcg ccttgcagcc
    12661 agcgatttgt cggcaatttc actactcatg cgctcgacga tatcggaggc ctgttcaagg
    12721 ttgagcgaca tttcgatgat ctccgcccag cggcgcgact cttcttctgc cagttcctct
    12781 tttggcatcc gcgccagata cagcttaatg gcggtataga gaacgttgat atcatctgcc
    12841 agcttacgca gctctttctc ctgccttggc tcgccgtgca tcactttatt cagcccttcc
    12901 atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc gcgcggcgtt cgccagagca
    12961 agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg ggtatccagt
    13021 tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac aaatggcagc
    13081 atggccaggc aacgtacaag gttgtagaag acgtggaaat agatcaccag ttccgctttt
    13141 ggcaatgaca acttccccat tgtctctgcc agcaaatgga caaacggcag gataatcagg
    13201 ctacccacca gcttaaacag cagactaccc agcgcgacac ggcgggctgc ggcattggcg
    13261 gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt tagcaccaat caccagacag
    13321 agcgccacgg ggaaggagat aatgcctgcg gcggtcagag tcgcggtcag cagtacagca
    13381 gcaaggctgg agtagctgat aatggcgaac atcgcgccaa tcagcgcatc cagcagaata
    13441 tcgccggtca gcgaggcaaa gatcacctga acgccgtttg cctgggtgat cggcgttacg
    13501 gcctgcacaa tcaactccag cgccagcaaa atcagcccaa gaccaatacc gacgcggccc
    13561 agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat aaaaataagt
    13621 aacggtgaca gccaggataa gtcgaaggtg agaatacgcg ccattagcgc cgtcccgaca
    13681 tctgccccca gcacaatgac cagagccggt gcgagggcta ccagatcctg agcgacgaac
    13741 gaggtcacca gcatggtggt ggcattactg ctctgtacca gtgcggtaac gccgatcccc
    13801 gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg caaacgcgcg
    13861 ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc cccagaccag cagggcgacg
    13921 gcagaaagca ggttaagcag cgttaacacg agttcaggtt ctccttatcg ttatatgttc
    13981 ctgaagcaat tgaatgacca aaggaccgga aacacgctga ctggcatctt tactgtgcct
    14041 tttgacattc gtttttccgg ctaagattat tccagcctgt ttccagtata agtgtttcat
    14101 attaataaag agacagggca ctcattgagc gccctgtttg ttgtaaggaa aagtgacaga
    14161 tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca gccagcgctc aacatcggag
    14221 acgctcatac ctttacggcg ggcatagtct tcaacctgat cgcgctgaat ttgcgccacc
    14281 gcgtagtact tgctgtccgg gtggctgaaa taccaacccg aaaccgatgc accgggccac
    14341 atggcgaaag attctgtgag tttcatgccg gtatgttttt ccacttccag cagctcccag
    14401 atggtggctt tttccgtatg ttccgggcag gccgggtagc ccggtgccgg acggatgccc
    14461 tggtagtttt cgcggatcag ttcttcgttg ctgaggttct cgttcggcgc atagccccag
    14521 tagactttac gcacacgctc atggagatac tccgcaaagg cttcggctaa acggtcggca
    14581 agcgctttca ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc atcagccagt
    14641 gcgtcctctt ccagcccgcc agtcacggca aatgcgccga tgtaatctgc tttaccagaa
    14701 agcttcggcg caacgaagtc agcgagacag tagttagcga atcccgtttt ttcggtctgt
    14761 tgacgcagat ggtggctgac gttgatcaca tgggtgcgcg tttcgtcacg gtagatttca
    14821 atgtcatcgc ccacacggtt tgccgggaac aggcccacca cgccacgcgg attcagcgtt
    14881 ttctcggcgc ttaatttatc cagcatgtcg ttggcgtctt taaacagccg ctgcgcctca
    14941 acgcccacca cttcatcttc cagaatgcgc ggatacttcc cggccagcga ccaggtcata
    15001 aagaacggtg tccagtcgat gtaattacgc agagtttcga tgctggcttc aacttcctgc
    15061 acgccgagac ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc gaagtcgtta
    15121 tcgcgcgccg cttccagcgt gaccggtggt gtgcgtggtt tcttgcgccc gtgctgaata
    15181 cgtacggttt cgtactcctt gcgggtacga gcgacaaaat catcgcgctg agtatcggaa
    15241 agcagcgccg ccaccacacc aacggtgcgc gaggcattct gcacatacac cgtcgggccg
    15301 ctgtagttct gctcgatttt caccgccgtg tgcgcttttg aggtcgtcgc gccaccgatc
    15361 agtaacggaa tggtgaagcc ctgacgctcc atctctttcg ccacgttaac catctcgtcc
    15421 agcgacggcg tgataagccc cgaaagtcca atcagatcag cattcacttc tttagcggta
    15481 cggagaattt tttccgcagg caccataacg ccgagatcga caatttcgta gttgttacat
    15541 tgcagcacca caccaacgat atttttaccg atgtcgtgga cgtcgccctt cacggtggcg
    15601 atcaccatct tgccgttggt tttgccctgc tctttgctgg cttcaataaa cggttcgagg
    15661 taggccactg cctgcttcat cacacgcgcc gatttgacca cctgtggcag gaacattttc
    15721 ccttcgccga acagatcgcc gaccacgttc atgccgtcca ttagcgggcc ttcaatcact
    15781 tcaatcgggc gcgtagcctg ctggcgggct tcttcggtat cctgctcgat aaactcagta
    15841 atgcctttga ccagcgagta ttccagacgt ttattcactt cccacgagcg ccactccgcc
    15901 tgctgggcgt tagcggtgtc gtcggttttg ctgccgcgat atttctcggc aagctccagt
    15961 aaacgctcag tgccatcatc gcgacgatta agaatcacat cttccaccgc gtcgcgcagt
    16021 tcagcgggca ggtcgtcgta aatcgccagt tgcccggcgt tgacgatccc catatccatg
    16081 ccattgcgaa tagcgtagta gaggaacacc gcgtgaatgg cttcgcgcac cggatcgttg
    16141 ccacggaatg agaaagaaac gttagatacg ccgccggaaa tcagcgcgtg cggcagttcg
    16201 cgtttgatgt cttcgcaggc gccgataaag tcctgcgcat agttgttgtg ctcttcaatg
    16261 ccggtggcga ccgcgaagat gttcgggtcg aagatgatat cttctggcgg gaagccaacc
    16321 tcttcggtga ggattttgta cgcccgacgg caaatctcaa ttttccgtgc gcgggtatcg
    16381 gcctgcccct gttcgtcaaa ggccattacc accaccgctg caccgtaacg acgcaacagt
    16441 ttcgcatgat ggataaaggc gtcgacgccc tctttcattg agatagagtt aacaatgcct
    16501 ttgccctgaa tacacttcag ccccttttcg atgacctccc attttgagga gtcgatcata
    16561 atcggcacgc gagcgatatc cggttcaccg gcaatcagat tgagaaaacg caccatcgcc
    16621 gcttcggcat cgagcatccc ttcatccatg ttgatatcga taatctgcgc gccgttttcc
    16681 acctgctgac gcgctacatc cagcgcctcg ctgtatttct cttctttgat caggcgcttg
    16741 aacttagcgg aaccggtgac gttggtgcgt tcacccacgt tcacaaacag gctatcgtcg
    16801 ccaatgttca gcggctccag gccggacaaa cggcaggcta cgggaatttc aggcagtttg
    16861 cgcggcgcta atccttctac tgcacgactc atcgctgcaa tatgttgtgg cgtggtgcca
    16921 cagcagccgc cgacgatatt gagaaaaccc gcttgcgccc attcacgtat ctgttttgcc
    16981 atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg gtagcccggc gttcgggtgc
    17041 gcggtgacgt agcattccgc aatccgtgat agttcctgca cgtactggcg cagttcatct
    17101 ggccccagcg cgcagttgag gccaaaagtc agggcttcgg catggcgcag ggagttgtaa
    17161 aatgcttcgg tggtctgtcc ggagagcgtg cgcccggagg cgtcggtgat ggtgccggag
    17221 atcataatcg gcagctcaac gcctaacgct tcgaactccg ttttcaccgc aaagaccgcc
    17281 gctttggcgt taagggtgtc gaaaacggtt tcaatcagaa tcagatccgc accgccttcc
    17341 actaatgctt tggtggattc gcgataggcc gccaccagct ggtcaaaagt gatgttgcgg
    17401 aatgccggat cgttcacgtc cggagaaatg gacgccgtgc ggttggttgg gccgagaacg
    17461 ccggcaacgt agcgcggttt ctccggcgtg cgcgcggtcc actcgtcagc acaggcgcgc
    17521 gccagtttcg ccgccgcaaa gttgatttcc gccgacaggg attccatctg gtaatccgcc
    17581 atcgcaatgg tcgtggagtt gaaggtgttg gtttcgatga tatccgcgcc cgcttcaaag
    17641 taggcgttgt ggatagcggc gatcacttcc ggtttgctta gcaccagcag atcgttattg
    17701 cctttgaggt cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc ttcgttcagt
    17761 cgatagctct ggatcatggt gcccataccg ccgtccagca ccagaatacg ttcatttaac
    17821 tgcgcacgca gttgttccac tttgctgctc acacttgctc ccgacacgct caacccagat
    17881 ttaataaaaa ttcaacaaac catactggca taaacgcatc tgtggtaaaa gcgaccacca
    17941 cgcaacatga gatttgttca acattaactc atcggatcag ttcagcaact attgcattag
    18001 ctaacaataa aaatgaaaat gatttccacg atacagaaaa aggagactgt catggtcgca
    18061 cccattcccg cgaaacgcgg cagaaaaccc gccgttgcca ccgcaccagc gactggacag
    18121 gttcagtctt taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc caatggcagt
    18181 gtggcactca cggagctggc gcaacaagcc gggttaccca attccacgac ccaccgcctg
    18241 ctaaccacga tgcaacagca gggtttcgtg cgtcaggttg gcgaactggg acactgggca
    18301 atcggcgcac atgcctttat ggtcggcagc agctttctcc agagccgtaa tttgttagca
    18361 attgttcacc ctatcctgcg caatctaatg gaagagtctg gcgaaacggt caatatggcg
    18421 gtgcttgatc aaagcgatca cgaagcgatt attatcgacc aggtacagtg tacgcatctg
    18481 atgcgaatgt ccgcgcctat cggcggtaaa ttgccgatgc acgcttccgg tgcgggtaaa
    18541 gcctttttag cccaactgag cgaagaacag gtgacgaagc tgctgcaccg caaagggtta
    18601 catgcctata cccacgcaac gctggtgtct cctgtgcatt taaaagaaga tctcgcccaa
    18661 acgcgcaaac ggggttattc atttgacgat gaggaacatg cgctggggct acgttgcctt
    18721 gcagcgtgta ttttcgatga gcaccgtgaa ccgtttgccg caatttctat ttccggaccg
    18781 atttcacgta ttaccgatga ccgcgtgacc gagtttggcg cgatggtgat taaagcggcg
    18841 aaggaagtga cgctggcgta cggtggaatg cgctgacttt ttctggcggg cagaggcaat
    18901 attctgcccg tcatacctga gtggtaatag aataagggtg tctgttaatc gcattgacgt
    18961 caaaataact tattgtcata cacttcatac acttttatta actcagcatt atttttaaac
    19021 atcaaaccac ttaattatta acaaaatcgc aagatctcat tccaccgaaa cttttacggc
    19081 tgcattatga gattgagcga tttatgatat ttcttta
//
LOCUS       DBFIFS010000069        18961 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.069, whole genome shotgun sequence.
ACCESSION   DBFIFS010000069 DBFIFS010000000
VERSION     DBFIFS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.069"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..133)
                     /locus_tag="N7A07_002225"
     rRNA            complement(<1..133)
                     /locus_tag="N7A07_002225"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            614..1306
                     /gene="yieP"
                     /locus_tag="N7A07_002226"
     CDS             614..1306
                     /gene="yieP"
                     /locus_tag="N7A07_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU2476884.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            1329..2756
                     /gene="mdtD"
                     /locus_tag="N7A07_002227"
     CDS             1329..2756
                     /gene="mdtD"
                     /locus_tag="N7A07_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU2476885.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(2722..3714)
                     /gene="rbsR"
                     /locus_tag="N7A07_002228"
     CDS             complement(2722..3714)
                     /gene="rbsR"
                     /locus_tag="N7A07_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU2476886.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3718..4647)
                     /gene="rbsK"
                     /locus_tag="N7A07_002229"
     CDS             complement(3718..4647)
                     /gene="rbsK"
                     /locus_tag="N7A07_002229"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU2476887.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4773..5663)
                     /gene="rbsB"
                     /locus_tag="N7A07_002230"
     CDS             complement(4773..5663)
                     /gene="rbsB"
                     /locus_tag="N7A07_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU2476888.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5688..6653)
                     /gene="rbsC"
                     /locus_tag="N7A07_002231"
     CDS             complement(5688..6653)
                     /gene="rbsC"
                     /locus_tag="N7A07_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU2476889.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6658..8163)
                     /gene="rbsA"
                     /locus_tag="N7A07_002232"
     CDS             complement(6658..8163)
                     /gene="rbsA"
                     /locus_tag="N7A07_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU2476890.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(8171..8590)
                     /gene="rbsD"
                     /locus_tag="N7A07_002233"
     CDS             complement(8171..8590)
                     /gene="rbsD"
                     /locus_tag="N7A07_002233"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU2476891.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8757..10625)
                     /gene="kup"
                     /locus_tag="N7A07_002234"
     CDS             complement(8757..10625)
                     /gene="kup"
                     /locus_tag="N7A07_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU2476892.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10848..12344
                     /gene="ravA"
                     /locus_tag="N7A07_002235"
     CDS             10848..12344
                     /gene="ravA"
                     /locus_tag="N7A07_002235"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU2476893.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12338..13789
                     /gene="viaA"
                     /locus_tag="N7A07_002236"
     CDS             12338..13789
                     /gene="viaA"
                     /locus_tag="N7A07_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU2476894.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13794..14786)
                     /gene="asnA"
                     /locus_tag="N7A07_002237"
     CDS             complement(13794..14786)
                     /gene="asnA"
                     /locus_tag="N7A07_002237"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU2476895.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14938..15396
                     /gene="asnC"
                     /locus_tag="N7A07_002238"
     CDS             14938..15396
                     /gene="asnC"
                     /locus_tag="N7A07_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU2476896.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15486..15929
                     /gene="mioC"
                     /locus_tag="N7A07_002239"
     CDS             15486..15929
                     /gene="mioC"
                     /locus_tag="N7A07_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU2476897.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            16308..18197
                     /gene="mnmG"
                     /locus_tag="N7A07_002240"
     CDS             16308..18197
                     /gene="mnmG"
                     /locus_tag="N7A07_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU2476898.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            18261..18884
                     /gene="rsmG"
                     /locus_tag="N7A07_002241"
     CDS             18261..18884
                     /gene="rsmG"
                     /locus_tag="N7A07_002241"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU2476899.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4746 a         4945 c         4677 g         4593 t
ORIGIN      
        1 agtttcccag acattactca cccgtccgcc actcgtcagc aaagaagcaa gcttcttcct
       61 gttaccgttc gacttgcatg tgttaggcct gccgccagcg ttcaatctga gccatgatca
      121 aactcttcaa tttaaaagtt tgatgctcaa agaattaaac ttcgtaatga attacgtgtt
      181 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
      241 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc
      301 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      361 tctgccggaa ttctcaggag aaccccgctg acccggcggc gtgcttgccg ttgttccgtg
      421 tcagtggtgg cgcattatag ggagttattc cggtctgaca agaggaaatt taaaataatt
      481 ttctgaccgc gcattttttc agcaaaagcc ccgttaatgt gccataaaac aagcaatttg
      541 ccgtttttgc aaacgtaatc acacttcctg gtggcatact aaggaggaat aaaaagaaaa
      601 aggaagatta gtaatgcctt taagcgcaca acagttagct gcacagaaaa acctttcgta
      661 tgttctggct gagaagctgg cgcaacggat cttaaaaggt gaatatgaac ccggcaccat
      721 tttgcccggt gagattgagc tgggcgagca atttggagtg agtcgtacag cggtacgcga
      781 agcggtcaaa acgttaacgg caaaagggat ggttttaccg cgaccgcgaa ttggtacccg
      841 ggtcatgcca caatcgaact ggaattttct tgatcaggaa ttgcttacct ggtggatgac
      901 agaagagaac tttcatcagg tcatcgatca ctttctggtt atgcgtatct gtctggagcc
      961 acaagcttgc ctactggcag caacggttgg caccgcggaa cagaaagcgc atctcaatac
     1021 gttaatggcc gaaatggcgg cattaaaaga gaactttcgc cgtgaacgct ggatcgaagt
     1081 cgatatggcc tggcatgagc atatctatga aatgagcgcc aatccgtttt tgacctcatt
     1141 cgcctcgcta ttccattcgg tttatcacac ttacttcacg tcaattacca gcgacacagt
     1201 gataaagctg gatctgcatc aggcgatagt cgatgcgatt atccaaagcg atggcgacgc
     1261 ggcatttaaa gcttgtcagg cgctgctacg ctcacctgat aagtgataac ccgataacag
     1321 gacattgaat gagcgataaa aagaagcgca gtatggcggg tttgccgtgg atcgcggcga
     1381 tggccttctt catgcaggca cttgatgcca ctattctgaa taccgcctta cccgcaatcg
     1441 ctcatagcct taatcgttct cctctcgcga tgcaatcagc catcatcagt tatacgctga
     1501 cggtggcgat gcttattccg gtaagcggat ggctagccga tcgcttcggt acgcgtcgca
     1561 tttttaccct tgccgtgagt ctgttcacat tgggttctct ggcctgcgca ctttctaatt
     1621 cgctaccaca gctggttgtc ttccgggtta ttcaggggat aggcggcgca atgatgatgc
     1681 ctgttgctcg gctggcctta ctgcgcgctt atcctcgtaa tgaacttctt ccagtattga
     1741 attttgtcgc catgccgggt ctggtggggc caattttagg ccccattctt ggcggcgtgc
     1801 tggtcacctg ggcaacctgg cactggatat ttttaatcaa tatccccata ggtattgcgg
     1861 gccttcttta cgcgcgcaaa catatgccca atttcaccac cgcacgacgc agattcgata
     1921 tcactggctt tttgctgttt ggcctcagcc ttgttctctt ctcaagcgga atagagctat
     1981 tcggggaaaa gattgtcgcc agctggattg ccttgacggt aattgtcacc agcatcgggt
     2041 tactgcttct ctatattctc catgcacgac gcacgccaaa cccattaatt tcattagatt
     2101 tatttaaaac ccgcactttc tcgatcggta tcgtaggcaa tattgcaacc cgtctgggga
     2161 ccggctgtgt accgttcctt atgccattaa tgttacaggt aggatttggt tatcaggcgt
     2221 ttattgctgg ctgtatgatg gcaccgacag cgttaggttc cattattgca aaatcgatgg
     2281 ttacccaagt cttacgtcgt ctgggctatc gccatacatt agtggggatc acggtgatta
     2341 ttgggctaat gatcgctcag ttctctttgc aatcaccggc aatggcaata tggatgctga
     2401 tcttgccgtt gtttatatta gggatggcta tgtcgacgca gtttaccgcg atgaatacca
     2461 tcacacttgc cgatctgacc gatgacaacg ccagcagcgg taacagtgtt ctggcggtca
     2521 cgcagcaact gtctatcagt ttaggcgtag ctgtaagtgc ggccgtcctt cgcgtttatg
     2581 aaggaatgga aggcacaacg actgtcgaac aattccacta tacgtttatc acaatgggca
     2641 ttattactgt tgcttcagca gcaatgttca tgcttctgaa aacaaccgat ggtaataatt
     2701 tgatcaaaag acagcgtaaa tctaagccga accacgttcc atcagaatcg gagtaagttg
     2761 taatcgttgt tgctgaaggg tcggctgggt tatccgatgg atgagtacat caatcgccag
     2821 ctcccccagt tcatcttttg gttgatggat cgtggttaat ggtggcgtca taaagcttgc
     2881 cagttcgata tcgtcatagc caatcaccgc gatatcctgc ggaacctgta actctgcctg
     2941 atataacgcc tggtaaacgc caacagccat agcgtcattt ccggtaaaga cggcctgagg
     3001 acgcagcgga tgtgatagca gttggcgcat agcgtcaaac ccgccgttaa attcaaaatc
     3061 accagtgact tcatagccat caggaatgtt gagacccgca cgtttcatcg ccgcccgata
     3121 accttccaac cgcaggcgcg ccggagtttt atccagcggg ccggtaatac aggcgatacg
     3181 ggtatgacct ttatcgatca gatattgcgt tgctaagtct ccgcccagca acgagttatc
     3241 ctgaataaga tcgctgtcgc catcgaacgg agcccagtcc atcatcacag taggcactgt
     3301 cggataacgt tgcatgattt cacgcgaagg ttgatgcgtt tcggtgcaca gtaacagcaa
     3361 gccatcaacg cgtttttgca tcagcgtttc cagattgcga ttcatccgct gttcatcgcc
     3421 ttcggtattg caaaggacga gactataacc gcgttcgaag cagctgcgtt caacgccacg
     3481 caccagttct gaatagaaag gattggtact ggcagtgatc aacatgccaa tggtatgtgt
     3541 ttgattgagt ttgaggctac gcgccagagc tgatggcgcg taattgagtt ctttaatcgc
     3601 cgcttcaact ttggcggtaa tcgcttcact gacgaagcga tctttattga taacgtgaga
     3661 aactgttgag gtagaaacgc ccgccaggcg ggcaacatct ttcattgtag ccaagcgtca
     3721 cctctgcctg tctaaaaatg cgtcgatctc ttcacgccac ggtacggaag gttgtgcgcc
     3781 tttacgtgtt acggcaatcg cagcggcagc atgggcaaac cgaatcgcct ctggcaatgg
     3841 tttttcttcc agcaacgccg tgattaacgc gccgttaaag gtatctccgg cagcaatggt
     3901 atcgacagcc tgcacccgga atccaggaac gcgctgacct tcaccattca cgctagccca
     3961 tacaccacga cttcctaaag taatcagtac agtacggata cctttttcat gcagtacctg
     4021 cgccgccttc gctgcatctt catcattttc aacacgaata ccggtgagct tttctgcttc
     4081 cgtttcgttt ggcgtaataa tgtccaccag cgccagcagt tcgtcaggaa gttcgcgagc
     4141 cggagccggg ttaagcgcaa cgatagtctt attttgatgg gcgattttcg ccgctgccat
     4201 cacactttcg agtggtgatt ccagctgcat taataatgct gacgcgttgg caatacgctc
     4261 acgttgcgct tccaccagcg ccggggaaag ggcagcatta gcgccggcat gaataccgat
     4321 gacattctca ccttcgccat taacaaaaat cagcgccaca cctgttgatt cgcctttgat
     4381 cacgctgacc ggagtaatat cgatgttatc agtggcgagc tgctggcgaa cgctctcgcc
     4441 aatgctgtca tcgccagtac aggcaataaa cgcgatattc gcaccgctac gcccagcagc
     4501 cacagcctga ttcgcgcctt tgccgccaaa tgcaacctga tagtggttac cggttacggt
     4561 ttcgcctgga gtaggaaaag attgaagatt aagaatgtgg tcagcattaa tgctgccaag
     4621 aacaacgagg ctgcctgcgt tttgcatatt cgggatgtcc ataaaatgcg ccaccgtgtt
     4681 agggtggcgc gtgccctgct tttctttata ttacacgttc atctgtcgat gacgtattta
     4741 tgtcaccatc aggtcataca acctgattaa aactactgct taacaaccag tttcagatca
     4801 accggatact tagcctgaac tttctcgcct ttcagcactt tatctgcggt ttcaacgcct
     4861 ttcgcgccaa tctgatcggg tagctgagcg atagtcgctg ctagtttgcc atcattcacc
     4921 gctttttcgc catccggtgt accgtcaaat ccgacgacca tcacatccga tttaccggca
     4981 gtttgcagtg cgcgcagcgc gcccagcgcc atttcatcat tctgcgcgaa tacagcctga
     5041 acatccggat gagcggtcaa caggttctgc attacgttca aacctttggt gcgatcaaaa
     5101 tctgctggct ggctggcaag aacattaaac ttgtgagcag caacggcctg ctggaagcct
     5161 tcgccacgtt cacgggctgc ggatgtacca gcaatgcctt gcagctcgat aactttggca
     5221 ccttcacccg ctttcttcgc gatgtaatca ccggcgattt tgccacccag tacgttatca
     5281 gaagcaatgt ggctcaccac ttcacctttc gttgcctggc ggtcaagagt gataaccggg
     5341 atgttcgcct ggttagccat cttcacagca ttacctactg cgtcggagtc ggtcgggtta
     5401 atcagcagaa ttttggtgcc gcgaacggtt aagtcctgca cgttcgccag ctctttcgcc
     5461 gggttgttct gggagtccag caccaccagg ttatagccaa gtttatccgc ctctttctgc
     5521 gcgccatctt tcagcgatac aaagaacggg ttgttaagcg tggagaccac cagcgcgatg
     5581 gtgtcttttg ccatcgcatt cgcactgacg gtggcgctta gcgcaacagc ggaaaccagg
     5641 gtagccagtt ttttcatgtt catattcaag atgtcctgta gtcgttatta ctgctttttg
     5701 ttgtctacca gcaccgccag caaaatcacc accgctttga cgatcatctg gtaataggag
     5761 gaaacaccta acaaattcaa tccattatta aggaagccaa gaattaatgc gccgatcaac
     5821 gtcccaacaa tgcgaccttt tccgcccgcc agactcgtac cgcccagaac caccgcagca
     5881 atagcatcca gctcatagcc agtccccgcc gtgggttgtg cggaggagag acgcgccact
     5941 tcaatgatcc cggccagtga tgccagcaga ccacaaagag aatagacgat gattttgatt
     6001 ttattgacgt tgataccaga aagacgcgtt gccgcttcgt tgccgcccag cgcgtagatg
     6061 taacgcccca gacgcgtgtg atgcagcatg taccaggccg cgaggaagac aatccccatg
     6121 atccagactg gcgtcggtac gcccagcgga cgaccaatac caaaccagcc aaacagatcg
     6181 gcgttctcag taaagccggt attcactggg ctaccgttgg tataaaccat ggtcacgccg
     6241 cgcagtaaaa gcatcataac cagcgtagcg ataaacgcct ggacgcgacc tttcgctaca
     6301 atcactccgg ttaccgcacc aattgcggca cctaacgcga gagcggcagc gacagccacc
     6361 agcgcattga cttcaatgcc gacgatagat gcagcaactg cgccggttag cgccaacaga
     6421 gaacctaccg acaagtcgat gcccgacgtc aggatcacca gcgtcatccc gaccgccata
     6481 atggcgttca ctgaggtttg ctggagaata ttgaataagt tattgatggt gaaaaagttc
     6541 gggcttaacg tcgagacaat cgcgatcagc accagcagag cgataagcga tttctgctcc
     6601 atcagccacg ctttcgtgaa ataacggcga ccagagacag tctgggttgt cattttttta
     6661 ctcctgattc acgcgattaa gcttgcccac agcggcagcc attaacactt cctgggtggc
     6721 ctgctcacga gtaaattccc cgctgagatg cccttcatgc atgacgatga tgcgatcgct
     6781 catgcctaat acttctggca tctccgatga caccagaatg atgctcaagc catcggcttt
     6841 gaactggtta atcagttgat agatctcttt tttcgcgccg acatctacgc cacgggtagg
     6901 ttcatcaagg atcaacactt tggggcgtgt catcagaccg cgggcaatcg ccactttttg
     6961 ctgattgcca ccggaaagca gaccaattgc ctgctccatc gacggcgttt tcacattgaa
     7021 caggcgaata aaatcactca cagcctgctg ttcatcggca tgcttcaaac tgccgccagc
     7081 gcggctgaag tagcgcagcg ctgtcagcga catgttctct tttactgaca tgcccaacac
     7141 taaaccgtca cgtttacggt cttcggagat atacacaatg ccgtttgcca ggccatcctg
     7201 cggtgaacgg gtaacgactt catgcccatc cagggtgacg taaccgctgg tgcgcggtag
     7261 tgcgccgtag agcactttca tcagttcggt acgacctgcg cccatcaaac cagagacgcc
     7321 aagaatttcg cctttgcgta aagtaaaaga gacatcgtta acgccaggtc cgcagagatt
     7381 atcgactttc aggcggatat ctcccggcgc tttgtccagg tgcggatatt gatcttccag
     7441 cttgcgaccc accatcatct caatcagcga atcttcggtc agtgatgcca cttcgcgctc
     7501 agcaataaat tgtccgtcac gaaaaacggt aacgtcatcg caactctcga agatttcttt
     7561 catgcggtgg gagatataga caataccgcg gccttgcgat ttcagctcgc ggatgacgcg
     7621 gaacagggat tcggtttcgg tatcggtcag cgcatcggtc ggttcatcca taatgatgac
     7681 tttcgactca aagctcagca ctttggctat ttcaaccatt tgctggtcac cgatggaaag
     7741 atcgcccacc agcttgtcgc ttttaaagcg caggttaagt ttagccagca atttatccgc
     7801 ttcggcatac atggttttcc agtcaatttt gccaaagcga ttaacaaact cacgaccgag
     7861 gaaaatgttt tcggcaatgg ttaactgcgg gatcaggttc agttcctgat ggataatccc
     7921 aatcccggct tcctgggaag atttcggccc ggtaaatgtc gtttctttcc ccagccataa
     7981 aagcgtaccg gcatcgcgag tatagatgcc agtaagcact ttcatcatgg tggatttacc
     8041 cgcgccgttt tcgcccacca gcgccatcac gcggcccgga tagacattta acgctgcgcc
     8101 cgagagggct tttacgcccg ggaaggcttt atcgatgcct ttaagctgaa gtaatgcttc
     8161 catgacggcc tcagaacgtc acgccagcac agagaatgat attcgcatac ggagaacatt
     8221 ctccgctgcg aattaccgcc tgactttctg cggtttgttg tttgaattgt tcatgcgtgg
     8281 tgtaacgaat ttcaatggta tttccctggt gtttttgcag ctgctcaagg tgagtgagca
     8341 acgtttcgtg gagttgcgga ttatggtgtt tgatctcttc cgcgataatg gcagcctcga
     8401 cctgcatttc atttgtgacg acgcccagca cctgcataaa agaaggtaca ccctgggtta
     8461 atgccatatc gatacgcgtt gtacttttgg ggatgggtaa accagcatca cacaccacca
     8521 gcgtatcggt atgtcccaga cgggagatca ccgatgaaat atcagaatta agaacggtgc
     8581 cttttttcat ttttttctcc atcagcgaaa cgtttcgctg acccgcagtt taatcctgac
     8641 tgagttggga aaaccatcgt gacgttacgg aaatgtgatc aacctcgaaa cgttttacat
     8701 ggtgattaac catgaaaaca aaaacgcccc cttatgaaga aaggaggcgt ctggcgttag
     8761 atttcgacct gagtacccag ttcgataacc ctgtttggcg ggatttcaaa ttgatctggt
     8821 gcacgcagcg cattacgttg cagcagcaag tacagcttgc cgcgcagacg caaataccac
     8881 gggcgtttgc cgaggatcaa cgactcatgc gacataaaga aggaggtttc catcatccgg
     8941 caacttaatc cttccagacc gcagcggtgg aaaacttctt ccacgtttgg cgtttctcgc
     9001 caaccataac ttgccaccac gcgccagaaa gtgggcgaca gttgttcaat ctgtacccga
     9061 cggacgttat ggacatatgg agcgtcttcg gtgcgcagag ttaacagaat cacccgctca
     9121 tgcaatacct tgttatgttt aaggttatgc atcagcgcaa agggaatgac gttgattgca
     9181 cgcgacatat ataccgcggt cccgggcacg cgaacaggcg gtgatttctc cagcgaagca
     9241 atcatcgctt ccagagagtt accatgttca tgcatccgcc gcagcaagcg gaaacgctca
     9301 ctcttccagg tggtcatcac gataaacatc accgtaccga ggctcaatgg caaccagccg
     9361 ccggagagca gtttatcgag gttagcggtg aacaatggaa tatcgacaca aaggaaagca
     9421 atcaagatca gcgcaacaaa atacttattc cagtgccagt tctgacgtgc cacggtagtc
     9481 gagagaatag acgtcagcac catggttccg gtcaccgcaa tcccgtacgc cgccgccagg
     9541 ttgctggagt gctcaaagct gacaatcaca atcacgaccg cgacatagag catccagttc
     9601 acaaacggaa tatagatttg ccctgactcc atttcggagg tgtgaataat gcgcatcggc
     9661 gacaaatatc ccagacgtac cgcctgacgc gtcaatgaaa agacgccaga gataaccgcc
     9721 tgcgaggcga ttaccgtcgc cagtgcggcg atgatcagca gcgggatcag cgcccagtcc
     9781 ggtgccaaaa ggaagaacgg gttcttaatc gcttccggat tctttaacaa cagcgctccc
     9841 tgaccgaagt aattaagggt caaggaaggt aatacgacgg tgaaccacgc caggcgaata
     9901 gggaacttac caaagtgccc catatcagca tacagcgcct cgccccccgt aatcgacagc
     9961 accactgccc ctaatgcaat aaaagaaacc gttttgtatt caaggaagaa atgcaccgcc
    10021 cacatcggat tcagcgcata cagcacttcc tggttagcaa taatgctacg taaccccagt
    10081 cctgccagaa tcaaaaacca ggtcagcatg atcggcgcaa acagcttacc gaccatagcc
    10141 gtgccatgct tttgaatcat aaataataaa gtgagaacga taattgagag gggaactatc
    10201 caggtatcca gctgcggggc gacgatttcc agaccttcaa tggctgacat caccgaaata
    10261 gcgggtgtta tgacgacttc accatagaaa aagctgccgc cgattagccc cataatcacc
    10321 agcattgatg tggttcgcgc cgacgtatta cgcccggcaa gcgacatcaa cgtcaggatc
    10381 cccccttcac cggcgttatc tgcccgcatc acgaaggtga gatatttaat ggaaaccaca
    10441 aagattagca gccagaagat cagcgataaa aagccaaaca cggcatcgcg ttcaacgcca
    10501 aaaccaaact ggccggacaa acattcacga agtgtatata acgggctggt accaatatcg
    10561 ccgtagacaa ctccaatcgc cgcgagggta atcgcgggca atgattgctt attatcagtg
    10621 ctcatagact agtctttcgt tgaaatatga aatgtgtgct tagtcccttg gcccacaaaa
    10681 aagcgcacag tatgcacgat taacggcaaa atcgtactcc taaatgcggc cacattaacc
    10741 tggctcaaag aaaaataccc cccctttgag actattttag ccaccagcaa gaaacgtcta
    10801 tactcgcaat ttacgcagaa cttttgacga aaggacgcca cttcattatg gctcaccctc
    10861 atttattagc ggaaagaatt tcccgcctga gcagttcgtt ggaaaagggg ctttatgaac
    10921 gtagccacgc catccgcttg tgtttattag cggcattaag tggtgaaagt gtgttcctcc
    10981 ttggcccgcc aggtattgcc aaaagtttga tcgcccggcg cttaaaattc gcctttcaga
    11041 atgcccgcgc gtttgaatat ctgatgaccc gcttctccac gccggaagaa gtttttggtc
    11101 ccctttctat tcaggcgcta aaagatgaag ggcgctatga acgtttaacc agcggttacc
    11161 tgccggaagc agaaatcgtc tttctggatg agatctggaa agcgggtccg gcaattctta
    11221 ataccctgct caccgccatt aacgagcgcc agttccgcaa cggtgcacac gtagaaaaaa
    11281 tcccgatgcg cctgctggtg gcggcctcca acgagctgcc ggaagcagac agcagtctgg
    11341 aagcgttata tgaccgcatg ctgattcgtc tgtggttaga taaagtgcag gataaagcga
    11401 atttccgctc catgctgacc agtcaacagg atgaaaatga caatccggtt ccagcctcct
    11461 tgcaggtcac agatgaagaa tatgaacgct ggcaaaaaga gattggtgaa attacgctgc
    11521 ccgatcatgt atttgagctg atttttatgc tgcgccagca actggataaa ttaccggatg
    11581 cgccttatgt ctcggatcgt cgctggaaaa aagcgattcg attattgcag gccagcgcct
    11641 tttttagcgg tcgcagtgct gttgccccgg tagatctcat tttgctgaaa gattgcctgt
    11701 ggtatgacgc gcaaagcctg aatttgatac aacaacaaat tgatgtattg atgaccggtc
    11761 atgcctggca acagcaaggg atgttgaccc gcctgggcgc gattgtgcaa cgtcacctgc
    11821 aactccagca gcaacaaagc gataaaacag ccttaacggt aattcgtctg ggcggcattt
    11881 tcagccgtcg tcagcagtat caacttcctg ttaacgttac tgcttccact ctgactctgc
    11941 tgctgcaaaa accgttaaaa ttgcataata tggaagtggt tcatatctcc tttgagcgta
    12001 gcgcgctgga acagtggctg agcaaaggtg gtgaaattcg cggcaaactg aacggtatcg
    12061 gctttgccca gaaactgaat ctggaagttg atagcgccca acatcttgtt gtacgcgatg
    12121 taagtttaca aggcagtacg ctggcacttc ccggttcatc ggctgaaggt ctgccaggtg
    12181 aaataaaaca acaattggaa gagcttgaaa gcgactggcg caagcaacac gctttattca
    12241 gcgaacagca aagatgtctg tttatccctg gcgactggtt aggtcgcatt gaagccagcc
    12301 tacaggatgt cggcgcacag attcgccagg cacaacaatg ctaacgctgg atacgcttaa
    12361 tgtgatgctg gccgtcagcg aagagggatt gatcgaagag atgatcatcg cgctgctggc
    12421 ctcaccgcag ctggcagtct tctttgaaaa attcccacga ctgaaggcgg caatcactga
    12481 tgatgttccc cgctggcgtg aggcgctgcg cagtcggctg aaagatgccc gagtcccgcc
    12541 ggaactcacc gaagaggtga tgtgctatca gcaaagccag ctcctctcca cgccgcagtt
    12601 tattgtgcag ctaccacaga tcctggactt actgcatcgt ctgaattccc catgggcaga
    12661 acaagcccga cagttggtcg atgctaacag cacgatcact tcagcgttac acacgctttt
    12721 tctccagcgc tggcgtttaa gtctgatcgt gcaagcaaca acgttaaatc aacagctatt
    12781 agaagaagaa cgcgaacaac tgttgagtga agttcaggaa cgcatgacgc tgagcggaca
    12841 acttgaaccg attctcgcag ataacaatac cgcagctggt cgtctgtggg atatgagcgc
    12901 cggtcagctt aaacgtggcg actatcagtt gattgtgaaa tacggtgaat ttcttaacga
    12961 acagccggaa ctgaaacgcc tggcagaaca actggggcgt tcccgggaag ccaaatcaat
    13021 accgcgcaac gatgcgcaga tggaaacctt ccgcaccatg gtgcgcgaac cggcgacggt
    13081 tcctgagcag gttgatggtc tgcaacaaag cgatgatatt ttacgtcttc tgccgccaga
    13141 actggcgaca ctagggataa cggaactgga gtatgagttt taccgtcggc tggtggaaaa
    13201 acagttgctc acctatcgcc tgcacggtga gtcgtggcgt gaaaaagtga tcgaacgccc
    13261 ggtggtgcat aaagattacg acgaacagcc gcgcggaccg tttattgtct gcgtggatac
    13321 ttccggctca atgggcggct ttaatgaaca gtgtgcgaaa gcgttctgcc tggctttgat
    13381 gcgcattgct ctcgctgaaa accggcgctg ttatattatg ctattttcca ccgagatcgt
    13441 ccgttatgag ctttcaggcc cacaaggcat cgaacaagca atccgttttt taagccagca
    13501 gtttcgtggc ggcaccgatc ttgccagttg ttttcgcgcc attatggaac gcttgcaaag
    13561 cagggaatgg tttgatgccg atgcggtggt gatttctgat tttatcgccc agcggttgcc
    13621 tgacgacgtg acgagtaaag tgaaagagct gcagcgggta catcagcatc gctttcatgc
    13681 cgtggcgatg tcggcacacg gcaaacccgg catcatgcgc attttcgatc atatctggcg
    13741 ctttgatacc gggatgcgaa gccgcctgct cagacgctgg cggcgataaa ttattacagc
    13801 agagaaggga cgctctcgcg aacagcagct ggccatactc cacactggac ctggccgata
    13861 tgcggcagtt gcagcagcag catggtcaaa cgagactggc cgataccgcc gccgatggtc
    13921 tgcggcattt caccgcgcag caacgcctga tgccattcca gctgcagacg atcttcatca
    13981 ccggtcagcg ccaactgatg cttcaacgtg tcggcatcca cgcggatccc catggaagaa
    14041 agctcaaacg catcttccag tacagggttc cacaccagaa tatcgccgtt cagacccgca
    14101 tggcccagct ctgacggggt gctccagtca tcataatccg gtgcgcgcac gtcgtggcga
    14161 tgaccatcgc tcagcttgcc gccaatcccg acaaggaata ccgcaccaag atctttcgct
    14221 atcgcccgct cacgcccttt ggcatcaaga tccggataac gagacagtaa ctcctggctg
    14281 tgtacgaagt ggatctgatc cggcaggaac ggtgccaggc caaactcttc gctaaccgca
    14341 gcttcggttg ctttaattcc cgcccaaatc gcctctaccg tgcttttcag agtcgagaat
    14401 tgacgctcac cgtcgcccat tacgcgttcc cagtcccact ggtcaacata gaccgagtgc
    14461 aacggagaaa gacggtcttc atcggggcga agggctttca tgtgcgtgta cagcccttcg
    14521 cccgcgctga agtcgtgttg ccctaaggtc tgacgtttcc acttcgccag tgaatgaacc
    14581 acttcgaact gggcatcagg cagagctttc acttttacct gtaccgcttt ttcacagccc
    14641 gacaagttat cctgcgtgcc atcccccaca cggctaagaa tcggcgcctg gacttcgatc
    14701 agccccagac gttcttccag ttgacgagaa aagtgagatt tcacgaagct aatttggcgt
    14761 tgtttggcaa tgtaagcggt tttcattttt tatactcctg cgtcctgttg cttatgatta
    14821 agcaacaaaa accgacacac atgcaataat cattcaataa aaagcctaac tcacttttga
    14881 ttcattaaaa aagaaggcta aaatagaatg aatcatcaat ccgcataaga aaatcctatg
    14941 gaaaattatc tgatcgacaa tctggaccgt ggcatcctgg aagcattaat gggcaatgcg
    15001 cgcaccgctt acgccgaact ggcgaaacaa tttggcgtca gtccggggac gattcacgtt
    15061 cgagtagaga aaatgaagca ggcggggatc attaccgggg cgcgtattga tgtcagcccg
    15121 aagcagctcg gttatgacgt aggctgcttt atcggcatta tattaaagag cgccaaagac
    15181 tacccttccg cgctggcaaa gctggaaagc ctcgatgaag tgaccgaggc gtactacacc
    15241 accggccact acagcatctt tataaaagtg atgtgccgtt cgatcgatgc tctccagcat
    15301 gtacttatca acaagatcca aacaattgat gaaattcagt ccaccgagac actgatcgtc
    15361 ctgcagaacc cgatcatgcg taccatcaag ccctgatcgg cttttttaat cccatacttt
    15421 tccacaggta gatcccaacg cgttcacagc gtacaatacg ccactcttaa taaaggtggc
    15481 ggtttatggc agatataact cttatcagcg gcagcaccct cggcggtgcc gaatatgtag
    15541 cagaacacct ggctgaaaag ctggaagagg cgggttttac caccgaaacg ctgcacggtc
    15601 cgctgttgga agatttatct gcctcgggga tctggctggt tatcagctcc acccacggtg
    15661 ccggagatat tccggacaac ctttctcctt tctatgaagc attgcaggaa cagaagcccg
    15721 atctttctgc ggtccgcttt ggcgcaatcg gtattggtag tcgtgaatat gacacatttt
    15781 gtggggctat cgataaactc gaggctgaac tcaaaaattc cggtgcaaaa cagacgggcg
    15841 aaacactgaa gatcaacatt cttgatcacg acattccgga agatccggca gaagaatggc
    15901 tgggatcgtg gattaattta ctcaaataag tatacagatc gtgcgatcta ctgtggataa
    15961 ctctgtcagg aagcttggat caaccggtag ttatccaaag aacaaccgtt gttcagtttt
    16021 tgagttgtgt ataacccctc attctgatcc cagcttatac ggtccaggat caccgatcat
    16081 tcacagttaa tgatcctttc caggttgttg atcttaaaag ccggatcctt gttatccaca
    16141 gggcagtgcg atcctaataa gagataacaa tagaacagat ctctaaataa atagatcttc
    16201 tttttaatac ccaggatccc aggtctttct caagccgaca aagttgagta gaatccacgg
    16261 cccgggcttc aatccatttt cataccgcgt tatgcgaggc aatcaccatg ttttatccgg
    16321 atccttttga cgtcatcatc attggcgggg gtcatgcagg caccgaggcc gcgatggctg
    16381 cggcgcgtat gggtcaacag actctgcttt tgacacacaa tatcgacact ctggggcaga
    16441 tgagctgcaa cccggcgatc ggcggtattg ggaagggaca tctggtaaaa gaagtggatg
    16501 cactcggcgg tctgatggcg aaagcgatcg atcaggcggg tatccagttt aggatactaa
    16561 acgcaagcaa gggaccggca gttcgcgcta cccgagctca ggcggatcgt gtgctctacc
    16621 gtcaggcggt acgtacggcg ctggagaacc aaccgaacct gatgatcttc cagcaggcgg
    16681 ttgaagatct tattgtcgaa aacgatcgtg tggtcggtgc tgttacccaa atgggactga
    16741 agttccgtgc caaagccgtc gtgctcaccg ttgggacgtt cctcgacggt aaaattcata
    16801 tcggtctgga taactacagc ggtggccgtg ctggtgatcc gccgtccatt ccgctttctc
    16861 gccgtttgcg tgaacttccg ctgcgcgttg gtcgtctgaa aaccgggaca ccaccgcgta
    16921 ttgatgctcg aaccatcgac tttagcgtgc ttgcgcaaca gcatggcgat aacccaatgc
    16981 cggtattctc ttttatgggc aatgcgtccc agcatccaca gcaggtgccg tgttatatca
    17041 cccataccaa cgagaaaacc catgatgtga tccgcagtaa cctcgatcgt agcccaatgt
    17101 acgcaggggt gatcgaaggt gtcggcccac gctactgccc gtcgatcgaa gacaaagtca
    17161 tgcgcttcgc cgacagaaat cagcatcaga tcttccttga accggaaggg ctgacttcta
    17221 acgaaattta tccgaacggt atctccacca gcctgccgtt cgatgtgcag atgcaaatcg
    17281 tccgctctat gcaggggatg gaaaacgcga agatcgtgcg tccgggttat gccattgagt
    17341 atgacttctt cgatcctcgc gacctgaaac cgacgctgga gagcaagttt atccaggggc
    17401 tgttctttgc tggtcagatt aacggcacta ccggttacga agaagccgct gcgcaaggtt
    17461 tgctggccgg tcttaacgct gcccgtctgt ctgatgacaa agaaggttgg gctccggcgc
    17521 gttctcaggc ctatctcggc gtactggttg atgacctgtg cactttagga accaaagaac
    17581 cgtatcgtat gtttacctcg cgcgcagaat atcgtctgat gctgcgcgaa gataatgcgg
    17641 atctgcgttt gactgaaatc ggtcgtgaac tgggcctggt ggatgacgaa cgttgggcgc
    17701 gctttaacga gaaacttgag aatatcgagc gtgagcgtca gcgtctgaaa tcgacctggg
    17761 taaccccgtc ggcggaagct gcagccgaag tgaatgctca cctgactgcg ccgctttccc
    17821 gtgaagccag tggtgaagat ctgctgcgtc gtccggaaat gacttatgaa aaattaacca
    17881 cgctgacgcc gtttgcccct gcgttgacag acgaacaggc ggcggaacag gttgagattc
    17941 aggttaaata cgaaggttat atcgcgcgcc agcaagatga gatcgaaaag cagctgcgta
    18001 acgagaacac cttgctaccc gcgacactgg attaccgcca ggtatccggt ctttctaacg
    18061 aagtgatcgc caaacttaac gatcacaaac cggcctctat cggccaggct tcacgtattt
    18121 ctggcgtcac gcctgcggcc atctccattc tgctggtgtg gctgaaaaaa cagggtatgc
    18181 tgcgtcgtag cgcataacgc attaaaaatg cctggtaagc ccccgcttac caggcaacgc
    18241 atcaagaaca ggtaatcacc gtgctcaaca aactctcctt actgctgaaa gacgcaggta
    18301 tttcgcttac cgatcaccag aaaaaccagc ttattgccta cgtgaatatg ctgcataaat
    18361 ggaacaaagc gtacaacctg acttcggtcc gcgatcctaa tgagatgctg gtacgccata
    18421 ttctcgatag cattgtggtg gcaccgtatc tgcaaggtga acggtttatc gatgtcggca
    18481 ccggtcctgg actgcctggc attccactct ctatcgtgcg tcctgaagcc catttcactc
    18541 tgttggatag ccttggtaaa cgcgtgcgtt tccttcgtca ggtgcaacat gagcttaaac
    18601 tggagaatat tgagccagta cagagcaggg tagaagagtt tccttcagag ccgccatttg
    18661 atggcgtaat tagccgcgct tttgcctctc tgaacgatat ggtgagctgg tgccaccatc
    18721 ttcctggtga gcaaggccgt ttctacgcgc tgaaagggca aatgccggaa gatgaaatcg
    18781 ctttgttgcc cgaagaatat caggtcgaat cagtggttaa acttcaggtt ccagccctgg
    18841 atggcgaacg tcatctggtg gtgattaaag caaataaaat ttaattttta tcaaaaaaat
    18901 cataaaaaat tgaccggtta gactgttaac aacaaccagg ttttctactg atataactgg
    18961 t
//
LOCUS       DBFIFS010000070        18883 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.070, whole genome shotgun sequence.
ACCESSION   DBFIFS010000070 DBFIFS010000000
VERSION     DBFIFS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.070"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(192..4094)
                     /gene="hrpA"
                     /locus_tag="N7A07_002242"
     CDS             complement(192..4094)
                     /gene="hrpA"
                     /locus_tag="N7A07_002242"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU2476900.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4295..4900
                     /gene="azoR"
                     /locus_tag="N7A07_002243"
     CDS             4295..4900
                     /gene="azoR"
                     /locus_tag="N7A07_002243"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU2476901.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4951..6243)
                     /gene="ynbD"
                     /locus_tag="N7A07_002244"
     CDS             complement(4951..6243)
                     /gene="ynbD"
                     /locus_tag="N7A07_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU2476902.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6257..8014)
                     /gene="ynbC"
                     /locus_tag="N7A07_002245"
     CDS             complement(6257..8014)
                     /gene="ynbC"
                     /locus_tag="N7A07_002245"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU2476903.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8030..8926)
                     /gene="ynbB"
                     /locus_tag="N7A07_002246"
     CDS             complement(8030..8926)
                     /gene="ynbB"
                     /locus_tag="N7A07_002246"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU2476904.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8926..9531)
                     /gene="ynbA"
                     /locus_tag="N7A07_002247"
     CDS             complement(8926..9531)
                     /gene="ynbA"
                     /locus_tag="N7A07_002247"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU2476905.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9702..12008)
                     /gene="ydbD"
                     /locus_tag="N7A07_002248"
     CDS             complement(9702..12008)
                     /gene="ydbD"
                     /locus_tag="N7A07_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU2476906.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIETFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12072..12932)
                     /gene="pdxI"
                     /locus_tag="N7A07_002249"
     CDS             complement(12072..12932)
                     /gene="pdxI"
                     /locus_tag="N7A07_002249"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU2476907.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13152..15611)
                     /locus_tag="N7A07_002250"
     CDS             complement(13152..15611)
                     /locus_tag="N7A07_002250"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU2476908.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15831..18809)
                     /locus_tag="N7A07_002251"
     CDS             complement(15831..18809)
                     /locus_tag="N7A07_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HGU2476909.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVKHNPVVYKNG
                     VTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSWTVHDDSNTVVFDGVNT
                     SGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQGGNTALNGGTVMTVDG
                     DNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGTAVIINGDNTKLNTAGD
                     STITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGATIKQEGDLYVSGGAHG
                     IDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLNVGQAAAGENAIGIQID
                     GDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFSTGLDVSGNNNNMTLAT
                     YELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNAQPYFFNPSTGVNISGDNND
                     VTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDDNTFTLNGGVHFVGEKNVMD
                     DGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGFENLIQLSHGAELEIGA
                     DATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNIGFAAAWNKDSTVINNG
                     SIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSVLNYPAEWNLSDGTSFN
                     NKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKSTADNEGQITLDTLWVDE
                     NDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHENGVITVYNAGAGMAAY
                     GNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAINDQTGVININAENGQAF
                     YNDGTGLIINYGTICTFGVC"
BASE COUNT         4753 a         5077 c         4269 g         4784 t
ORIGIN      
        1 ttatccgttc aaattgcgac ctcagtaaaa tatcatgtct actcattgca atacgtcctg
       61 ttcgcccaag gcgaagtatg aatgaaactt atgtaaaata gtaccgccct tgctatattt
      121 cagaattcat aaagaaaaaa cccggcgcta aatgcttacc gggttttaga tttatcaggc
      181 aaatagcagg gttaaccgct aatctgctcc attgcctgca aaatacgctt atctgaaatc
      241 ggataaggcg taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa
      301 cggatctctt tcacgtcttc atcctcacga cgtgcaggcg ggagtttgtt gaaccattgc
      361 tgccacgcct gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca
      421 accgccagtt tttccagccg tttttcaatc gcctgcaaat aacgcagcgt gtcgcccagc
      481 cgtttgaagc cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta
      541 atgtcagaaa gccccagcgc catggtcata tccacccgcc ctttcagacg tttgttgata
      601 ttgaacactg ccgtaaggat ttgctcgacc tgcttcgcaa tatccaccac cgtgtcgttc
      661 agttcggcac gcactttttc atgcagcgca gcaaagcctt cttccgtcca gaccgggcct
      721 ccattggcgt cgatcagttg atccacaccg catgagatac agtcgtcgat cagctccagc
      781 actttgccat acgggttaaa gtacagcccc agcttggctt tgtttggtaa cttctcatgt
      841 aaatacttga ttggtgaggg aatattcagc agcagtagac ggcgaagacc gttccacatt
      901 gcctgttttt gctccagcgg gttatcaaac agtttgatcg ccacactgtc gcgctcatcc
      961 accagcgccg gccacgcctt cactttgtag ttaccacgct tctgttcgta gctttccggc
     1021 aactgaccaa aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgca
     1081 gatagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct
     1141 tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc
     1201 acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg
     1261 cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg
     1321 gcatagtttg gcgcaggtac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga
     1381 gcaatcacca gttcgcggcg cagaccaggg atctgccatt caaacccgct ttcctcaacc
     1441 tggttaagta acggcagcgg aatatgtacg gtcacaccgt cagcatccgc gccgggctca
     1501 aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc
     1561 ttgctgatct tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca
     1621 ggcgtttcgc ggctgacttt tttccaccag ctgtcgaagt gacgagcgga gattacatcg
     1681 tggccgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg
     1741 cgacgtgatt tgtgttcaag ctcttccact tcggcccgca gtttcaggtt ttcacggaag
     1801 aatgcgtgac gcgtctgcca gtcaccttcc accagcgcgt ggcgaataaa gagttcacga
     1861 cataacgccg gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca
     1921 taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg
     1981 ctgtaggtac gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc
     2041 gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc
     2101 ggcggttttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg
     2161 gtatattctt gtttatcggc atctttcatg ccgatatggg aaagcaaccc cgtcagcaac
     2221 gcaatgtgaa tttcacgata ctccgccggt tcgctgttca ccggaatgcc gagttctttc
     2281 accacctggc gcaactgggt gtagatatcc tgccattcgc gcacacgcag atagttgaga
     2341 taatcggtac gacacaggcg acggaaggcg ttggaagaaa gcgccttttg ctgctcgcca
     2401 agataattcc acagattcac aaacgcgagg aagtcagact ctttgtcgtg gaagcgacga
     2461 tgtttttcgt ccgatgcctg ctgtttgtcc atcggacgtt cgcgcggatc ctgaatggag
     2521 agcgcagacg tgataatcat cgcctcacgc acgcagccat gtttttgcgc ttccagcacc
     2581 atacgcgcca gacgtgggtc gacaggcaac tgcgagagct ggcgaccgag cggcgtcagt
     2641 ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg
     2701 ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgcct
     2761 agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga
     2821 tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct
     2881 tcggacacac gaccacagcg gcctttacgc tgattggcag acgcctgcga aatgggctca
     2941 atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg
     3001 tcgataacgt atttaatccc cggtacagtc agcgaggttt ccgcgacgtt ggtcgccagc
     3061 acaatgcgtc gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc
     3121 gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta
     3181 tcgcgaattt cccgctcgcc gctcataaag atcagaatgt cgccggggct ttcctggctt
     3241 agttcgtcca cggcatcgaa aattgcctgc aactggtcgc gctcagtgtc gtcggcttct
     3301 tcaacaatcg ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcaataatc
     3361 ggcgcattat taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga agtgataatg
     3421 atttttaggt caggacgccg cggcagcaac tctttcaaat aaccgagcag aaaatcgata
     3481 ttcaggctac gttcgtgcgc ttcgtcaata atgatagtgt cgtactgcat cagcaggcgg
     3541 tcttgctgga tctccgccag caggataccg tcggtcatta gcttgaccat cgtgttatca
     3601 cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc cgttttcagc
     3661 tcttccgcaa tacggttcgc taccgttctt gccgccaggc gacgcggctg ggtatggccg
     3721 atcagccctt taatcccgcg ccccagctcc atacagattt tcggtaactg agtcgtttta
     3781 ccagaacccg tttccccggc gactatcacc acctggtgat cacgaatcgc ttcgagaatg
     3841 tcctgttttt tctgactaac cggcaaattg tcaggataag taatttccgg tcgtgccgct
     3901 tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg
     3961 gcctgttgtg catcaggatt tttaaccttc ttcacgccgt gcagacggcg agaaaaacgc
     4021 agtctgtcac gcagcatcag cgaatccagc cgctgctgca aggccgtaaa agtcaatttt
     4081 tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc tttgaaatga
     4141 tagatataga gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc
     4201 ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcaac aagcaacggg
     4261 gcatcgccca ttcaaatatc tataaggaaa caccatgagc aaggtattag ttcttaaatc
     4321 cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg
     4381 gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccaattcc
     4441 ggtactggat ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactacgcg
     4501 tcagcaggaa gctctggcac tttccgatga gctgattgcc gagctgaaag cccatgacgt
     4561 tatcgttatt gcggcaccga tgtataactt caacatctcg actcagctga aaaactattt
     4621 tgacctggtt gcacgtgcag gcgttacttt ccgctatacc gagaatggtc cggaaggtct
     4681 ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc
     4741 aacggacctg gtgacgccgt atctgtccac gttcctgggc tttatcggca ttaccgatgt
     4801 gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc
     4861 tgacgcgaaa gcagccatcg acagtattgt tgctgcataa ccggacacga tcttgatgtg
     4921 gactgtagtt cagtccacat ctcaatccac ttacctgttt tcccataatt tcaacatcgc
     4981 tttgtgctct tccttaagca caatatgcga gcgtctggct cgaataaagc taatcgcttc
     5041 atcaactgtt ttacagtgtc cgtaacataa cagccatgcc gccactacca gcgcactgcg
     5101 cgataatccc aatgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag
     5161 catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac
     5221 acagaaatag agtcgatctt ttgtcgctcg tccgcgaggg aattcaaagg tgacatccag
     5281 aaccgcattc tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtcat
     5341 tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gatagccaca tcccgatgcg
     5401 ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt
     5461 tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca
     5521 acataaccag actgaccacc ataactgaac catcatcatt agctccatca acacaatgca
     5581 cgcgcacgcg cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg
     5641 ataattccaa cgacggtcga cgggtatcat ccagtcaatc aacataccta ccgccagccc
     5701 tgtgatgaca tcaataaaat gatgctgcca ggttgtcagc atcgaaatgg cgatgagtaa
     5761 aaaccatccg ccgcagactt tacgccacct cacagccaga tgctgacgaa agtgacgcca
     5821 aagtagccag cagagaacaa tatgcagcga aggagactgg ttataaggca gatcaaacag
     5881 ttcaagttgc gaaaatagcc atcctgtcac cccactcact tcaggacgga taaaactaaa
     5941 cttcagcggg tagagaaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg
     6001 gacaagtcgg cgctgttcga atgtcgagct acaaacgaac agcgaaaatc catataaaag
     6061 atccagactc cagtaaggga caatagtcca gggaagaaaa gggatcgccg tttcccagcc
     6121 gaatacctga ctggggatat catggctgtt aaagtcctga accgcggtga actgatttag
     6181 agatccatag gtgaagaaga aaaacggggc cagcaacaat aaccagccag cgccttgtag
     6241 caatacgttc cggcgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc
     6301 actcatcaat ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct
     6361 ccccttgcga acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc
     6421 cggcaatcag ctccagttgt gggtgccacg gctgcccggt gtagaggaga atgccgcccg
     6481 gttcgatggc attggcaaga cctgcgagtg agttttttac ctgctcattt ccgggaaaaa
     6541 gctcatacag tccagagaca atcgccagcg taggccgcgg agttaacgcg ctgagttcct
     6601 ccgggttaaa cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgagcaa
     6661 tcatctcttg cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta
     6721 cggcaggctc gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa
     6781 ccacgcggac ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt
     6841 gtaaatgggt tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt
     6901 cgattaagcg cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg
     6961 aactgcctga atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga
     7021 acaatttcat cgctttacgc ataaagcgat acgccaaatc aaccggcgat aatggcacgg
     7081 gtccacctga aaggagccgc cagcgatccg ctgatggtcc agtgcggtct tcatgctgat
     7141 aatcaaattt ttgtgacttg ttagcatata aacgactaat aaagctttgc attttttcaa
     7201 atgcctgcgc cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca
     7261 gctctttcag agggctacgt aatctatgat aaaaatcaat ctgcggttgg cgatgcacca
     7321 catagtcatc gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa
     7381 caatacgttc agacgtttta taaagatcga gcaagatatt aacggcaatc gcccgtgtga
     7441 tcagcggatc attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt
     7501 tcacatagga attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac
     7561 gtgccagcgg cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa
     7621 ttgcaggcgc ataatcatga acccatgtgg caaccagcac tgcgccgacg ctctgcgcga
     7681 tcactaccac ctcttccagc ccgacctggc tgtcgctggc agcaaaacgg acaaactcat
     7741 cgacatcccg cactgaacgc gcaagagatg ggctataacc acggggcccc gaagtttgtc
     7801 catgccctcg ggcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga
     7861 tatgttgtag acgaccagaa tgttcatgac cgcgatgaaa taagacgatg acctttttcg
     7921 cccctggctg taaagcgggc cagtgccgat aaaacaacgc tgtattatca ctggtggtaa
     7981 aaaaatgttc cccagggatg cgtgaatttt ccattttctc tattttcctt cagtaacagc
     8041 agtagcgaat aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa
     8101 ggagtccgcc atgtcctggt aatagttttc cgctatcttt aacacccaca tctcgtttga
     8161 cggctgacat cactacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata
     8221 atgcctgtaa tgtatttagc ggtgtcagca acggtccaat aatcagtgac gcaatcatgg
     8281 tggtgattac gccccccacc agaccttcca gcgtttttcc cgggctgact ttggggacca
     8341 cttttcttct gccgcaggat ttcccccata aatactgtgc aatatcgttt gactctgtta
     8401 acgccaaaag aaataacacc agtaacgccc cgccctgggt atttgtggtt gtcaacatta
     8461 ataaccaggc ggcatgactc aaggcgaaaa cggtcattat ccagccccaa aaaatggcgc
     8521 tcacggtatg cagaaaaccg gaggtatcac ctactaaaac tcgccaggtg gttaatatca
     8581 gaaacccagc caaaggtata aataacagaa acagctcaaa gcaactaaaa ccgataagca
     8641 aatagttaag aggaataacc caatataacc aacgcggaaa atgtacggat ataagcgtac
     8701 aatattcttt caatgccaga aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg
     8761 gacctgaaat caccagtgaa aaacaaatga cgatacccca ccaggtacga atacgtagtg
     8821 taacttcatc cccttttctc tccggtaacc gccatacccg aaaacggttt atcagcgttg
     8881 ctaaaattaa caacgcgaaa agtgttgcca gagatttttc cagcattatc tttcagcact
     8941 catcaggagc acactccgac aacgattaat cgcagtccag agaagcagaa ttgaagcaat
     9001 gctccataaa agattattcc attgcatcca ctgtggataa atggcaacgg ccagccccca
     9061 caacccaaat attaacgcgc gatcgctttt gccaaaagga ccgacataac tacgaatacc
     9121 gttaatcgtc tgcgcgagta aaccgcaaaa ctcagtcaat atggtgcaaa ataacatgag
     9181 tattacgagt gatgcgttac tttccggtaa aaataaaaac ggtaaataga gcgcaatatc
     9241 ggaaataaca tcgccagttt catttaaaat cgcccccaga cgtgtttgct gattgcactc
     9301 acgcgccaac atgccatcca gcgcattgag tgccatacgg ataaaaagca cgatgggcaa
     9361 tagcaaaaag aggatgggtt gtgcgaccaa catcaacagc aatccggtaa taagagaaag
     9421 cgccagtgca gcaagagtga tgtgattcgc tgtaacatgg tgcttataaa gccaaaacat
     9481 cgtcggcctt aacagcgact gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc
     9541 ttgataattc ggtttgcgct atggtgcatc aattcccctg acggagatat cgcaacacga
     9601 cgcttcatta aaaattcaga atatatcaag gtattgggga ctcactgtca ggaatgatta
     9661 tccccaataa atcaagtggt taaaaattat tacgctgtta ttcaatacaa ctcatcaagt
     9721 tttctcatcg cttgttcccg tattgcgcgc gtcttaccgt taagcgccag ctggcgaact
     9781 aaatccagtt cagggttaac aagctttaac gccagcaaag gatcacgttt cagcctcgcc
     9841 tttaatacat caactggaaa acggggatta ttcatcgcca caatccacca ctcgggatca
     9901 atttctgccg ccagtactgc aggcggtgtg tggcggttgt taagtgcgcc gaacttgaaa
     9961 aatgtgagat tgcttttcgc cagtgtactc caccatatac gatccggttg ttttgccagt
    10021 acatccagtt gtgctaacgc atcggggtct agtgcataat aaacacgtaa cgcttcgtta
    10081 acctgcgcac gggaactgtt ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt
    10141 ggcgtgtttt gctgtaattc cttgagaaca acagcgggta aattatccca actaattaat
    10201 gcactctcta aaaagtccca gtcgtccgaa cgtatcagag aggtggcatt taatagcatc
    10261 tggtcatcaa cttcaccgta gctgataagc tcagacaaaa tcatatcagc gctggtttca
    10321 tatgcatctg aatctgcttt gggattattt ttccgatgcg ataaagcacg ttgccaaatt
    10381 ttttgtttca tcgacgtgct cagatactga tttttcgcca gattactcca cccctcagga
    10441 tcgttgaaac gtgtcaataa ccgttgtaaa gcagcgtcag agtgaattat ctgcatatcc
    10501 cgacagcaaa agtaggcagg ggattttttc tccatccttt taacaaaatt aaactgtaag
    10561 ttatctggca acgctaaaaa taacgcacct attagatcat cgtcattctc gaatttttgg
    10621 tataaatata ctgctatttt gtcactctct tgtggcgtca tccgcaacaa ttgaccatgg
    10681 tgtttcatct ctgccagctt taacgctaag cttgtgacaa catcgtttga tgaatcgttc
    10741 gccaactgca ttgcctgatt gtaggtgagt tgtcctccag caccgacctg acgaatagag
    10801 gaatcatcac tgcggatata gccatcaata atggattcgg gcaataacca cgcatcttta
    10861 taaagctcct gaatgatcat atcccaatct cttttcgcca gagtcatgac gatatccaga
    10921 gggagatttt cacgtagcgc aagattcaac ggaccgacac catcaggtag atgactcatt
    10981 cgcgtcatta tttcgacaga gagcggttca cggtcgagca accccagaac aacaggctgg
    11041 taacccaatt gctgatcggt caactccaga atctcattgt caggcatata tctcgccata
    11101 ctttcaatcg tttgtccatc attgctatta ataagttgtt tgcgtaggtt agctgaaatt
    11161 cctttaagcc ttgggagagt tcgcattatg gcaagatcaa atgtcgcaat atctcgtcct
    11221 tcaaatgcag caacgttgcc ttcatccagc atttgcaatt cctgcactaa ctcaggagaa
    11281 ggcttaaggc gaaacagcgc ttcttttttc atctgattac agacattccc tgccatgcga
    11341 atattgcttc gcttaaaaat ttctttcaga gcatcttcat catttattag accccaataa
    11401 tatgcatcag gatttattat catcagcgct tttttgacat tttcagtgtc atttttgtca
    11461 ctcgcacgga ccatgaattc actatttgtc gaagacgcaa caacaggtgc aattatgtca
    11521 ttgataaata tcccacgcgc attaatactc cccttaatcg tagacggatt atcgtaatat
    11581 gagtcgataa ataccagtcc acttgcattt atcgaaccgg taattaacag tggtgaatcc
    11641 gtaactatat tcgttgccgt cacgttaccg agaataaccc atggattaga aagagagaaa
    11701 gtggaaatat tacctttgac tgtcaaattg ccaagaacaa tcaatttttg attactctct
    11761 atattacctt ctaccactaa atcgccacga tgcactttcc agtccgatgc agataaaccg
    11821 aaggggacaa gattcacgct actgttgata gcttgttcaa cttgttgttt atcttcagcg
    11881 gtagccgcag aaaaaaaagt ttcaatatca actgcatttg caacgcatcg aagttccgcc
    11941 tgtactggaa gactcagcat aaacagggga agtaaaccta tccagctgca aagcctgggc
    12001 ttaaacatat gcactccatg ccattaaaaa agaaacaaaa gggaaatgca tctttccctt
    12061 ttgtatattc gttattctcg cgaaatacca tccaacgtag acaacacttt ctcagaaaga
    12121 tgcaactttt cagcagccat attctcccgt aaatgcgcaa cagaagacgt ccctgggatc
    12181 agcaaaatat tcggtgaacg ttgtaacagc cacgccagcg ccacctgcat tggtgttgca
    12241 cccaagctcg cagcaacatc ggaaagcgtg gaagattgca gcggcgtaaa gcccccgagc
    12301 ggaaagaacg gcacgtaggc aatgccgtca cgagccaaag cttcaatcat tgcgtcatca
    12361 gcacgatgag caatgttgta ttcgttctgc acacagacga tttcggcaat cttgcgcgcc
    12421 tctgcaacct gcgtcggtgt gacgttgctt aggccaatat gttttaccag gccttgttgt
    12481 tgcatctctg ccagcacggt caggctggct tcaatcgatc cttccgccgg gccatgacca
    12541 tcccccatca tcacgcgcag gttaaccaca tccagtacgt ccaggccgag attacgtaga
    12601 ttatcgtgca ccgctttttg cagctctgcc ggagaaaatg cgggcaacca ggatgcgtcc
    12661 tctccacgcc gcgcaccaat tttagtgaca attgtcagat cgtcagagta aggataaagc
    12721 gcttcgcgga taatctgatt ggtgacgtgc ggaccataaa agtcgctggt atcaatgtga
    12781 ttgacgccca atgccagcgc ctcacgcagc acggttatag cgacgtgacg atctcttggg
    12841 gggccaaaaa ccccaggacc tgccagttgc atcgcgccat aaccaagacg gttaacggat
    12901 tttgtaccga gagtaaatgt attgctgctc atcgcaccct gccctgtaat aaaacggatt
    12961 aagaaaaatc agtatagaaa cgaaaagaga taaataatgc gaaggcgtaa acaagatttc
    13021 tggatgagta gcactattgt cctgaaagac accaaaacat tagacagcaa aagataagtc
    13081 aagcgtcgca tccggcaata aaaaaccgca acctgtatca gcgttgcggt ttgtgtatca
    13141 catgctaaaa attagaacgt tttcttaaag ttcagcatca cgcctttgtc gctgatgccg
    13201 tcctctttcc agtgatacgc atccagattc agcgaacttt ctttgctact gtacttcacg
    13261 ccgacgctcg ccaggctgtt gataccgccg cccttctgac cgtcatcgac gttaaagtgc
    13321 tggctgcctg cccctgcaag gcttgccgtg cgctggctct tgctgtagct caggttcggc
    13381 ccgccttcca gcgtcgcatt cgcgctccag ccgcctttcc ctgcgtagtc cagtttcagc
    13441 ccgacgatgc tgtccaccgc cgtttcgctg ccgctgttca tgctcaggtt aaagtctccg
    13501 gcattgcgct cctgatagcc gccttccagc gagtgacgca gtttcactcc ggcgtacggg
    13561 gtgattttca gcccttcgcg cggctcaaag gttttcgccc cttcgctacg caactccagg
    13621 tactgctgtt tcacatccgt atccgccgtt ttgctgacgt cgccgtaagc gaccgagcgg
    13681 ctgctgtcga gattatgcac gtcataacgc agcgcgttgt tccaacgcat accattgtcg
    13741 aaggacatct ggtgcttcag gccaaagaac tggctgtagc cgccggttac gccattgtcg
    13801 cccgctttct gcgcaccgtc gccatccaga cgcgcgatac cgtattccag gctcatgctc
    13861 tggctttcgc tcaggtcaac ggttttacgc agtgccagca tgtcatactc ggtgttattt
    13921 ccgagttccg cacgcgggtc acctttcgcc accacgttaa aggccaggcc attgcccacc
    13981 ttcggcgcgg catccgccag catgctgaag cggttgctta acacacgcgc ttcacggaat
    14041 accgtggtcg cctggctacc gctcacctgc ttcagggcgc tattcagttc agcagtggtg
    14101 cccacgttca ggctggtata cagctcgtta ttggtgtaac ccgcatccag tgccttcgcc
    14161 acatcgttca ccgaagcatc ggtcgccaca tcggtgtagg cgtttttgct catgatgacg
    14221 tcaacgttgc cgctggcatc ggtgctgcct ttggcggtcc ataccacgga cgttgaggtg
    14281 atggcatcgg cgtcggtcag gttgatgcct tccaccacgt tgtcgaaact gaccgtggtg
    14341 tctgccgtac cggcggcgaa accggtgttg atcccgacac cattcatgct ggcgttgttg
    14401 accttcagtt taccggcgct gccgtcaacg ttggtaccga cgatatagcc gttgaggttg
    14461 ttactgctgc catcgctact accgctatca gtcgtttcac tgaaaggtga agtggtgtaa
    14521 gtgtttggca tgtccggcaa cgtgtagtcg gtgtaatgca cttcggtatt gttaccgctg
    14581 ttgccgttca ccccttccac attcacgctg tcgccctgct taatcaaccc cgaaccagtc
    14641 acgccgtcgg caatcaccac cgtaccgttg ttaacgatat gaccttctgt gcccagtacg
    14701 ctgaacgcaa acgagtcgtt agcaaaaata ttgatcgtgc cgttgttctc aattaccgca
    14761 tcggcggtgg cattggcatc cagttgcata ccagtcaatc cggtatcggt ggtgccttcg
    14821 gtaccgagat tgataacgcc gttgttaacc agctgtgcgt ttttatcgcc ttccatcgcc
    14881 acggcgttgt tgccgctgat gttcattaca ccatcgttga gagcaacata gttgttgtgg
    14941 gtgaatttaa ccgcgctggc gttgtcttta tcgaagttaa ttaccccgcc atcctggttc
    15001 cacaagaagg catcgctttc tgtcgcaccg gcagtactta ccgccacggt gactttcgca
    15061 tccaccacgc ccttgttaat aaactcgctg gcgccatcgt cgtagtgaat agcgcccgca
    15121 cctaatgcgg catccagcgt catttcaccg gtgttatata acgtggcgtt agcattgctg
    15181 ccctggaagc gaatggcgtt tttgctgtta tcaaggatga gtttgccgct gttgttgaac
    15241 gttgacttat cgtcaatatc cagcgtcccg gcattgttgt tttcatcctg ataacccatt
    15301 ttccaggtac ccgcgttatt cactaccgca gagttataga gaatcgcgcc tttacgcgcc
    15361 tcgccgcctg caaggttatt gaataccgca tcgccctgag cgttaacgcg cgccgacgca
    15421 ataccgctgt tattcaccgt accatcgtga agttgtaaag ttgacaggga accggtgtta
    15481 ttaattaccg cgttgctgtt tttctcaatt tttacgacgt cattaagcgt tccgttgtta
    15541 ttgaaggttc ctccttccag agtcaccgtg ttcaatacac cttcattggt taacgcaccg
    15601 ccttcattca cgacgatggc tgagctaatg gttccgctcg actcgttaac cagcgcacca
    15661 ttttcgagta tcctgatcgt tccattttta acagcacccg cactggtgac atccccgttg
    15721 ataatcgcac ctgacgataa taatacggtc tcacccgcag aacttaatac agcgccatca
    15781 tcatataaat cagcaatata attatccgtt ggattatatt catcggcccc ctagcaaacg
    15841 ccaaaggtac aaattgtgcc atagttgata ataagcccag tgccatcgtt atagaatgcc
    15901 tgaccatttt ccgcgttaat gttgattacg cctgtctggt cattaatggc ggtgccttcg
    15961 tgataaaccg ccatgccgac gagtttattc acaccgagag aatcgttgta attttcattt
    16021 ttctccaggt tgatggttcc ctggttaatg accgtattag tattcccata cgccgccatc
    16081 cccgcaccgg cgttgtagac agtaatcaca ccattctcgt ggttggttgc cgtggcttta
    16141 gttaatgccc cattataggc atcggtacct gaagccattc ccacaccgaa atctttcgcc
    16201 gtgctatttg caacattatt acgcagcgat gtttcatcat tttcatcaac ccacagcgta
    16261 tccagggtga tttgcccttc gttatccgcg gttgatttat cgatggctag catccctaca
    16321 ctgccacggc catacatatc aataataccc gtttcgccgt tcagaatgga tgacgagtag
    16381 ctggcaagca tacctgtcag ccctaatgcc ttgttattga aagaggtacc atcagacaag
    16441 ttccactctg ccggataatt cagcacactg tgctgattca tcattttggt ggtcatggta
    16501 ccattgttaa cgccaatccc accgtattca acatcaaaga cttttggtgc cgggtcggta
    16561 ccaaaatcgt acatcgccag gccaatggat ccgttattga ttaccgttga atctttattc
    16621 caggcagccg caaaaccgat atttttcagt tccacttcac cgttgtttgt cgccttcgca
    16681 ccagaatcaa tactaatggt ggataacgct acacgataat aataagtgaa agaatccaca
    16741 tcactcatat caaacgttgc gtcagcgcca atctccagct ccgcgccatg gctcaactga
    16801 atgagatttt cgaaccccag cgggaactcg ccgctgatgg ttgactcacc attcaggtag
    16861 accggggaat gcccatcaac gttaataagc ggtgtgtctc cgataccacg tcgagaagct
    16921 gatggttttg agccatcatc catgacattt ttttcaccga caaaatggac accgccatta
    16981 agcgtaaagg tattgtcatc gccagcaata acaatacctg caatgtgttc ctgagcaccg
    17041 tcgtaactcg cataggaacg cgacgtggtt ttactatcag caacaacggt taactgacca
    17101 tccagcgtga cgtcgttgtt gtcaccgctg atatttaccc cggttgaggg attaaagaag
    17161 taaggctgcg cattatcggc tttctgatct ttatcaacca gaatactgcc cgctatttcg
    17221 atagtattac catcacccga aacattaaca cccgtcgctt tttgccctgt cacattcagc
    17281 tcataagtcg ccagcgtcat gttgttatta ttgccagaga catcaagacc ggtggagaaa
    17341 tccccaacgt ctaacccacc cgcgagtgaa atattagctt tatccccagc aacgctgaca
    17401 ccggttccgg cattggtcgc ggaaatatca ccaacgttaa caaatgtcgc attatcaccg
    17461 tcgatctgaa tgccaatggc attttcaccc gctgcggcct gaccaacatt gaggtcgccc
    17521 atgttgatca cgctgacgcc atcaccatca agcagcatgc caatagagtt atcttcgatg
    17581 accgtaatat tacctttgtt gctaacgaac gtatcattgc cgtcaacgtc aataccgtgc
    17641 gcaccaccgc ttacgtataa gtcgccttcc tgtttgatgg tggcaccgtc accgacaatt
    17701 ttcgagccag tggaattctc tccgtcaacg ctcatggtac cttgattatc gacccgcgca
    17761 ttatcgccat tgataaggct acccgtacca ccattggtga tggtcgagtc gcccgcagtg
    17821 tttagcttgg tattatcgcc attgatgatt accgcggtac cgccatcaac ttccgtctcg
    17881 ccattattgt taatggtgat attgtcgcca gtgagtatac cgacaaccga gccttctccg
    17941 atggccgtcg ttttgccgtc gttgttgagg gagatgttgt caccatcaac cgtcatgaca
    18001 gtaccgccat tcagcgccgt gttgccgccc tgatcggtga tgatcgcgtt accaaactca
    18061 atcgcattcc cggttgtgcc gtcaacaacg acgccggcct ctttagtgat gtggatgagt
    18121 ccatcatcgt cgtaactcca ggtgatgcca cctgaggtat ttacgccatc aaagacaacg
    18181 gtgttgctgt cgtcatggac tgtccaggac ttaactatcg tctccttgcc ctcaggcgcc
    18241 gttaaggtgt agctcccatc ggcattttgg ctgtaggtaa aggtagtttc gcgaattttg
    18301 acagtttttg catcctgatc ccaagttacg ccatttttat agacgacagg attgtgttta
    18361 accggggtat cgccaccatc gtctggcgga actggcgtat caccgccgtc atctggcgga
    18421 accggcgtat caccgccgtc atcaggcgga actggcgtat caccgccgtc gtctggcgga
    18481 acaggcgtat caccgccatc atccggaact ggggagttat tatggtggtg attgtcatcg
    18541 tcacttaagg tatatatagc aattcccgta gctactgcag caagacctgc agctgcccat
    18601 ggtaaaactg cactatcttt attttctctg cactccgcgg ataaattttc tagctgctct
    18661 ttgctcaggg aatgaatatt tgccggacat gttatcttac tggacttacg tgttttttcg
    18721 gttgagtcgg gtattgaagt atccgcagcc catgtctggc tacttaacgc catggcaaca
    18781 caaacagaca ataatttttt cctttgcata taaaattcct tagatctaga ccgtgcgtaa
    18841 caagccatgc agaatttgaa gataaatcgt agtaaatatt cat
//
LOCUS       DBFIFS010000071        18518 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.071, whole genome shotgun sequence.
ACCESSION   DBFIFS010000071 DBFIFS010000000
VERSION     DBFIFS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.071"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            236..541
                     /locus_tag="N7A07_002252"
     CDS             236..541
                     /locus_tag="N7A07_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476910.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            648..1292
                     /gene="gfcB"
                     /locus_tag="N7A07_002253"
     CDS             648..1292
                     /gene="gfcB"
                     /locus_tag="N7A07_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU2476911.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1289..2035
                     /locus_tag="N7A07_002254"
     CDS             1289..2035
                     /locus_tag="N7A07_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU2476912.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2035..4131
                     /locus_tag="N7A07_002255"
     CDS             2035..4131
                     /locus_tag="N7A07_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU2476913.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4177..5316
                     /locus_tag="N7A07_002256"
     CDS             4177..5316
                     /locus_tag="N7A07_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU2476914.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5304..5750
                     /gene="etp"
                     /locus_tag="N7A07_002257"
                     /gene_synonym="yccY"
     CDS             5304..5750
                     /gene="etp"
                     /locus_tag="N7A07_002257"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU2476915.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5770..7950
                     /gene="etk"
                     /locus_tag="N7A07_002258"
     CDS             5770..7950
                     /gene="etk"
                     /locus_tag="N7A07_002258"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU2476916.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8070..9374)
                     /gene="appA"
                     /locus_tag="N7A07_002259"
     CDS             complement(8070..9374)
                     /gene="appA"
                     /locus_tag="N7A07_002259"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU2476917.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9454..9546)
                     /gene="cbdX"
                     /locus_tag="N7A07_002260"
     CDS             complement(9454..9546)
                     /gene="cbdX"
                     /locus_tag="N7A07_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU2476918.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9559..10695)
                     /gene="appB"
                     /locus_tag="N7A07_002261"
     CDS             complement(9559..10695)
                     /gene="appB"
                     /locus_tag="N7A07_002261"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU2476919.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10707..12251)
                     /gene="appC"
                     /locus_tag="N7A07_002262"
     CDS             complement(10707..12251)
                     /gene="appC"
                     /locus_tag="N7A07_002262"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU2476920.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12385..13242)
                     /locus_tag="N7A07_002263"
     CDS             complement(12385..13242)
                     /locus_tag="N7A07_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU2476921.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13239..13637)
                     /gene="hyaE"
                     /locus_tag="N7A07_002264"
     CDS             complement(13239..13637)
                     /gene="hyaE"
                     /locus_tag="N7A07_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU2476922.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13634..14221)
                     /gene="hyaD"
                     /locus_tag="N7A07_002265"
     CDS             complement(13634..14221)
                     /gene="hyaD"
                     /locus_tag="N7A07_002265"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU2476923.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14218..14925)
                     /gene="hyaC"
                     /locus_tag="N7A07_002266"
     CDS             complement(14218..14925)
                     /gene="hyaC"
                     /locus_tag="N7A07_002266"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU2476924.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14944..16737)
                     /gene="hyaB"
                     /locus_tag="N7A07_002267"
     CDS             complement(14944..16737)
                     /gene="hyaB"
                     /locus_tag="N7A07_002267"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU2476925.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16734..17852)
                     /gene="hyaA"
                     /locus_tag="N7A07_002268"
     CDS             complement(16734..17852)
                     /gene="hyaA"
                     /locus_tag="N7A07_002268"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU2476926.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18278..18365
                     /locus_tag="N7A07_002269"
     tRNA            18278..18365
                     /locus_tag="N7A07_002269"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18312..18314,aa:Ser,seq:tga)
BASE COUNT         4527 a         4920 c         4802 g         4269 t
ORIGIN      
        1 acaattttct tatctttaat ttgtattttg ctaagattta tcttatgctc acaggtcgta
       61 tttccagagg ggaatagtta acaatcttat atttccctct gcgcgccttt ctcacccgct
      121 gtatatagtg caagtggtat gaggaaaggg agatttcaca aggatgttat actcaaagta
      181 gacaatcctg atctcaagcg tacgtattgt ccatgcgatg aataaaggaa aagttatgaa
      241 acacaagctt tctgcaatcc ttatggcctt catgttgacg acgccagctg cgtttgctgc
      301 ccctgaagca gcaaacggga ccgaggcaac gacgggtact actggcacca caacgaccac
      361 taccggtgca accacgacgg ctgctaccac tggtggcgtg gctgctggcg ctgtcggcac
      421 ggcgacagtt gtcggtgtgg caacggctgt tggtgtggcg acgttggcag tagttgcagc
      481 aaatgacagt ggcgatggcg gttctcataa cacctcaacc accacgtcca ctacgcgcta
      541 attctgctta aacgcgtcga aattaaagtt aaataatacc cggttgcgat attcgtaccg
      601 ggctttagag atttgcattt ttttaattaa cctttcaagg attaaaggtg cgccctctta
      661 ttttatcgat tttcgcacta tttcttgcgg gatgtacgca cagccagcaa agtatggtcg
      721 atacatttcg cgccagcctt ttcgataatc aggatatcac cgtagcggat cagcagatcc
      781 aggcgttgcc ttattccacg atgtatttac gccttaatga agggcaacga atctttgtgg
      841 tactgggata tatagaacaa gaacaaagca aatggttatc ccaggataac gccatgctgg
      901 ttacccacaa tggacgtctt ttaaagaccg tcaaacttaa taataatctg ctggaagtga
      961 caaattccgg gcaggaccct ctgcgtaacg cgctggcaat aaaagacggc agccgctgga
     1021 cgcgcgatat tctctggagt gaagacaacc attttcgctc tgcgaccctg agttccactt
     1081 tttcctttgc tggattagag acgctgcata ttgcgggtcg cgatgtgctg tgtaatgtct
     1141 ggcaggaaga ggtgacttcc acgctaccag aaaaacagtg gcaaaacaca ttctgggtcg
     1201 attcggctac tggccaggtt cgtcaaagtc ggcaaatgtt aggcgcaggg gttattcccg
     1261 tagaaatgac gtttcttaaa cccgcaccat gaataaatta cagtcgtatt tcattgccag
     1321 cgtactttac gtaatgacac cccatgcctt tgcgcaagga acggtgacta tttatctgcc
     1381 tggcgaacaa cagacacttt ccgttggccc cgtggagaat gttgcccagt tagtgacaca
     1441 accgcaactg cgcgatcgcc tctggtggcc tggtgccctg ctgaccgact ccgcggtgaa
     1501 agctaaagcg cttaaagact accaacatgt catggcacag cttgcttcct gggaagcaga
     1561 agctgacgac gatgtggcgg caacgattaa atccgtgcgc cagcagttac tcaatctcaa
     1621 tattactggt cgcttaccgg tcaaactgga ccctgatttt gtacgagtgg atgagaacag
     1681 caatcccccg ctggtcggga actatacgct ctataccgta cagcgcccgg tgactattac
     1741 tcttctgggt gcggtttctg gggctgggca attgccctgg caggcaggcc gtagcgttac
     1801 cgattatctg caagatcacc cccgtcttgc aggcgcggac aagaataacg ttatcgtgat
     1861 tacgcctgag ggtgagactg tcgtcgcccc tgttgccctg tggaataaaa gacatgtgga
     1921 accaccgcct ggcagccagc tttggttagg cttttctgcg catgtactgc ccgaaaaata
     1981 tgctgattta aatgaccaga ttgtgtctgt tttaacgcaa cgtgttcctg actgatgaag
     2041 aagaattctt atcttttaag ctgcctggcc attgccgtct ccagtgcctg tcatgctgaa
     2101 gtattaacct acccggatcc gctgggttcg tcgcaatcgg actttggcgg cacaggattg
     2161 ttgcagatgc caaatgcgcg catcgcaccg gaaggtgaat tcagcgtcaa ctaccgggat
     2221 aacgatcaat accgtttcta ctccacctcc gtggcgctgt ttccatggct ggaaggcacc
     2281 attcgttata cggatgtgcg cacacgtaaa tatagccagt gggaagattt cagcggcgat
     2341 cagtcataca aagacaaatc attcgatttt aaacttcgcc tgtgggaaga aggttactgg
     2401 ctaccgcaag tggcgtttgg taaacgtgat attgctggta cgggtctgtt tgacggtgag
     2461 tatctggtgg ccagcaagca agcggggcca tttgatttca ccctcgggat ggcatggggc
     2521 tacgccggta atgcgggcaa tattaccaac ccgttttgcc gggtgagcga taaatattgt
     2581 catcgcgcag agtctcacga tgcgggcgat atcagcttta gcgatatctt tcgtggcccg
     2641 gcttccatct ttggcggcat tgagtatcaa acgccgtgga atcccctgcg tctgaaactc
     2701 gaatacgacg gcaacaatta ccagaatgat ttcgctggca aactgcctca ggcaagccat
     2761 ttcaacgtcg gtgcagttta tcgcgctgcc agctgggcag acctcaacct gagttatgaa
     2821 cgcggtaaca cgttgatgtt tggtttcact ttacggacca atttcaacga tctgcgccct
     2881 gccctgcgcg atacgccaaa accggcatat caacctgcgc ctgaatctga aggattgcag
     2941 tacaccacgg tagcaaacca acttaccgcc ctgaagtata acgcgggctt tgacgcgcca
     3001 gaaattcagc tacgcgataa gacgctgtat atgtctggtc agcaatacaa ataccgtgac
     3061 tctcgtgaag cggtcgatcg tgccaaccgg attctggtga ataacctgcc gcaaggcgtt
     3121 gagaagatta gcgtgacgca aaagcgcgag catatggcga tggtgactac cgaaaccgac
     3181 gtagccagcc tgcgcaaaca gctggcaggt acagcgcctg gtcaatcaga gccactgcaa
     3241 caacaacgtg ttgaagctga agatctttct gcctttggtc ggggctaccg tattcgtgaa
     3301 gatcgcttta gctactcttt caacccaaca ctttcacagt cgctgggcgg cccggaagat
     3361 ttctatatgt tccagctggg gctgatgtcc agcgcccgct actggtttac cgaccacctg
     3421 ctgcttgatg gcggtatttt caccaatatt tacaacaact acgacaagtt taagtcttcg
     3481 ctgttgcccg cggactctac cctgccccgc gtgcgcacgc atatccgtga ttacgttcgc
     3541 aatgacgttt atctcaacaa cttgcaagcg aattactttg ccgacttagg caatggtttc
     3601 tatggccagg tgtatggcgg ttatctggaa acgatgtatg ccggtgtcgg ttccgagctg
     3661 ctttatcgcc cgctagatgc cagctgggcg ctgggtgtgg acgttaacta cgtgaagcag
     3721 cgtgactggg acaacatgat gcgcttcacc gattattcca cgccaactgg tttcgtgacg
     3781 gcttactgga acccgccgac gctcaatggc gtactgatga aacttagcgt tgggcaatat
     3841 ctggcaaaag ataaaggggc aacgatcgac gtcgccaaac gctttgacag cggcgtggcg
     3901 gtaggggtat gggcggcaat cagtaacgta tctaaagatg actacggcga aggcggcttt
     3961 agtaaaggtt tttatatctc gattccgttc gacttgatga ccattggacc taaccgcaac
     4021 cgcgcggtgg tttcgtggac accattgacg cgtgatggtg gacaaatgct gtcacgcaaa
     4081 taccagctct atccaatgac ggcagagcga gaagtaccgg ttggacaata acctgacgta
     4141 attcataaca ttacattgac tgataaatat aaaccaatga aaaagaacat ttttaagttt
     4201 tcggtactga cattggctgt tctgtctttg acagcatgta cccttgttcc tggtcagaat
     4261 ttatcgacca gtaacaagga tgtcattgaa ctacctgaca atcagtatga cctggacaaa
     4321 atggtcaata tctatcccgt cacgccagga ttgattgatc agttgcgcgc caaaccgatt
     4381 atgtcgcagg ctaacccgga gctggagcag caaatcgcga actatgaata tcgtatcggc
     4441 attggtgatg tgttgatggt tacggtctgg gatcacccgg aactaaccac ccccgcaggg
     4501 caataccgta gcgccagtga taccggcaac tgggttaacg ccgatggtgc gattttctac
     4561 ccctatattg gtcgcctgaa agtggccggg aaaacactta cccaggtacg caacgagatt
     4621 acagcgcgtc tggattcagt cattgaaagc ccgcaagtcg atgtcagcgt cgcggcgttc
     4681 cgctcgcaaa aagcgtacgt caccggtgaa gtcagcaaat cgggtcagca gccgattacc
     4741 aatattcctc tgaccatcat ggatgcgatt aacgccgcgg gcggtctgac cgcagatgca
     4801 gactggcgca atgtggtgct cacccagaat ggcgtaaaga ccaaagtgaa cctctatgcg
     4861 ctgatgcagc gtggcgactt gcgccagaac aaactgctgc acccgggaga tattttattt
     4921 attccgcgca acgatgatct gaaagtcttc gtgatgggtg aagtgggtaa acagagcacg
     4981 ctgaaaatgg accgcagcgg tatgaccctg gcggaagcgc tgggtaacgc tgagggtatg
     5041 aatcaggatg tcgctgatgc gacggggatt tttgttattc gtgcaacgca aaacaagcag
     5101 aatggcaaaa tcgccaatat ttatcagctg aatgcgaagg acgcatcggc aatgatactc
     5161 ggaacggaat tccagcttga accttacgac attgtttatg tcactaccgc gcctctggct
     5221 cgttggaacc gtgtgatttc cctgctggta ccgacgattt ccggggtgca tgacctgaca
     5281 gagacttcac ggtggattca aacatggccc aactaaaatt taactcaatc ctggtggttt
     5341 gtaccggcaa catttgccgt tcgcccattg gcgagcgctt gctgcgtaaa cgtctgccgg
     5401 gcgtaaaagt caaatcggca ggtgttcatg gtctggtaaa acaccctgca gatgcgacag
     5461 cggcagatgt tgccgctaat catggcgtat ctcttgaagg acatgccgga cgtaagctca
     5521 ctgctgagat ggcacgaaat tacgatctga ttctggcaat ggagtcggaa catattgctc
     5581 aggttaccgc aatcgctccc gaggtccgcg ggaagacaat gctgtttggg caatggctgg
     5641 aacagaaaga gatcccggat ccctatcgta aaagtcagga tgcatttgaa catgtctacg
     5701 gaatgttgga gcgcgccagt caggaatggg cgaagcgcct cagccggtaa tttgagttta
     5761 taaaatacga tgacaactaa aaatatgaat acgccaccag gcagcactca ggaaaatgag
     5821 atcgatctgc ttcgtctggt cggcgagtta tgggatcacc gtaagtttat tatcagcgtg
     5881 accgcgttat tcacgctgat cgctgtcgct tactcgctgt taagcacacc aatttatcag
     5941 gcagatactc tggtccaggt tgagcaaaaa cagggcaacg ccattctcag cggcctgagt
     6001 gatatgatcc ctaactcatc gcccgagtct gcaccggaga tccaactgct gcaatcgcgc
     6061 atgattctcg gtaaaaccat tgctgaactg aatctgcgcg acatggttga gcagaagtat
     6121 tttccgattg tgggtcgcgg ctgggcgaga ttaaccaaag agaaaccagg tgagctggcg
     6181 atcagctgga tgcatattcc acaactgaat ggtcaggatc agcaactgac actcacggtt
     6241 ggggaaaacg gccactatac gctggaaggt gaagagttca ccgtcaatgg tatggtcggc
     6301 cagcgtctgg aaaaagatgg cgttgcgctg actatcgcgg atattaaggc caaaccagga
     6361 acacagtttg tcctgagcca acgtaccgaa ctggaagcga ttaacgcgtt gcaggaaacc
     6421 tttaccgtta gcgaacgcag taaagaaagc gggatgctgg aacttaccat gactggtgat
     6481 gatccccagt tgattactcg tattctgaac agcatcgcta acaactattt gcaacagaat
     6541 atcgctcgcc aggcggcgca ggattcacaa agccttgaat tcttacagcg ccagttgcca
     6601 gaagtgcgca gcgagctgga ccaggcggaa gaaaaactca acgtttatcg ccagcagcgc
     6661 gattcggttg accttaacct ggaagccaaa gccgttcttg agcagattgt gaacgttgat
     6721 aatcaactca atgagctgac cttccgcgag gcagagatct cccagctgta taagaaagat
     6781 cacccaactt atcgtgcgct gctggaaaaa cgccagacgc tggagcaaga acgcaaacgc
     6841 ctgaataagc gggtatcggc tatgccttcc acccaacagg aagtgttgcg tttaagtcgt
     6901 gacgtagaag cgggccgtgc ggtatatctg caattactta accgccagca ggagttgagt
     6961 atttcgaaat ccagtgccat tggtaacgtg cggattatcg acccggcagt cactcagccg
     7021 cagccagtga aaccgaaaaa agcgttgaat gtggtgcttg gttttattct tggcctgttt
     7081 atttcggtgg gtgccgtgct ggcgcgtgcg atgttgcgtc gtggtgtaga agccccggaa
     7141 caactggaag agcacggcat cagcgtttat gccaccatcc cgatgtccga gtggctggat
     7201 aaacgtaccc gtctgcgtaa gaaaaattta ttttctaatc agcagcgcca tcgtactaaa
     7261 aatatcccct tcctggcggt ggataacccg gcggattctg ctgtggaagc cgtacgtgcg
     7321 ctacgaacca gtctgcattt cgctatgatg gagactgaga ataacattct gatgatcacc
     7381 ggtgcgacgc cagacagtgg taaaacgttt gtcagttcaa ctctggcagc ggtgatcgcc
     7441 cagtccgatc aaaaagtgtt atttattgat gccgacttac gccgtggtta ttcgcataac
     7501 ctgtttaccg tgagtaatga acatggcttg tcggaatatc tggcaggtaa agatgagctc
     7561 aacaaagtga tccagcattt tggcaaagga ggctttgatg tgattactcg cggtcaggtg
     7621 ccacctaacc catctgaact gctgatgcgc gatcggatgc gtcaattact ggaatgggcg
     7681 aacgaccatt acgatctggt gattgttgat acgccgccga tgctggcggt gagcgatgct
     7741 gcggtcgtgg ggcgctctgt cggcaccagc ctgctggttg cgcgttttgg cttgaacacc
     7801 gccaaagagg tgagcttgtc aatgcagcgt ctggaacagg ctggcgttaa tattaaaggc
     7861 gcaatcctca atggtgtgat taaacgcgcc agcaccgctt acagttacgg ctataactat
     7921 tacggttata gttactccga gaaagagtaa aactgaaggt aaagtgaccg gatgcggcgt
     7981 gaacgccttt tccggtctac gcattagaca gttcttcgtt tgtcatcagc ctcagagcat
     8041 tcaggtagct gaatgctctt ttttatacat tacaaactgc acgccggtat gcgtgcttca
     8101 ttcacgattt gcgtaaaacc ggccaacgaa cacatgccct gcgcatttcg ctcttcacat
     8161 cctgccaggg tcagtttcac ctctccgggc ggcgtattta atgacagcgg agttttatca
     8221 cgcatctgct gtaaagtctg gaagaccagc gaaacctgaa tccactggct gttatcgctt
     8281 agccgacgcc agcgttcaaa caccagttca ccacctggcg gcgtgttatc cggctgacct
     8341 ggaagcgtcc agttgagctc cagtgcgccg ccgagatttg ccagattagt atcgtgtccg
     8401 gcgataaaca gcactgaagt gggtaatgtc acaccatacg cctgtttttg cggtggatgg
     8461 ggagtcaacg ctatcatgat caaatccaac aacggggtgg cgcggctgcg ggcaacctct
     8521 ggcgtgcgtt gtagtaaata aaattgcgcg ttatgcaaac ttagcaacgt gttccactgg
     8581 tgtgaatcag tgatccttcc ccaccccggc tccggcattc cctgtgcctg ttgcaggaga
     8641 aatatttccg tcagcattga tgcgaggctt accgcaccgg ttaatgagac attgtcggcg
     8701 ctcaccttga gttccgatgg taatgcctgc gttaatgaac agctttcgtc ctgtttctca
     8761 cggttaaggc acaagtttga ttgcggaaaa ttaagcaccc gttccagttc gcgaaacgcc
     8821 gtttgccgat gcccggtaaa gtcagcaatt gaccctcctg ccctgctgag gatcgcgtca
     8881 gtcacgttcg cgttatccag ttggcaaacg ccagttttta gaggattaaa taacggatcg
     8941 ggactggacg tatctgcctg ggtatgtacg gttattgcac agtcaggtgc cagcccggcg
     9001 gcgaaggctt cgcctgtttt acgggtacgc tcgtcgacat cagcaataat cgcgacctga
     9061 ccaggctgcg ggcagccctt tttcgtcagc aatccgtcgg ctaccagacg ctggcgttgg
     9121 taatgtccga gataagcgat tagctcacca ccgcgcggtg tcagccaacc cagttttacc
     9181 ggccagtttg gccatgcgtc tggggtgaca tcctgcatca gttgcgtggc cttggttggg
     9241 gcacgcacac catgacgact gacaatcacc acactttcca gcttcagctc cggctccggc
     9301 tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa aaatgggatc
     9361 aagatcgctt tcatcgatat gtttccgctt ttcatatccg acattattga ttgcctgata
     9421 cgacgctaat gcgccgcatc agacttacgt tcgttaactt ttcaatcgcg ggtccagcca
     9481 taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc aaagtaaata
     9541 ccacattgtt cctgctcctt agtacagctc gttttcgtta cggcggagag tttctgtcgt
     9601 catgcgcccc cacattttgt agtagctcca gagagtgtag agcaacacaa tgggcaaaaa
     9661 tatcagcacg attaccaaca taatactcag cgtcagctga ctggaagtgc tgtcccacaa
     9721 cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca gcgtgatgcc
     9781 tgccgtgaaa atcacgccaa attgcatcaa tgacgccatc aaaaatcccc aacccgggcg
     9841 gccacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca gcgggaagat
     9901 ccacaaaacg ggcgactcga caaaattatt catccaggca ccaggtagca ctgccaccag
     9961 tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa agccatcaat
    10021 accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca gtgcagcgcg
    10081 tttagtcgac agctgtgaac gcagatgaat cacaccaaca gttttcagtt gtaaccagac
    10141 gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag ggaatggcgt
    10201 cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggagtga aggcaaacgg
    10261 cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca ccagactgcc
    10321 gatgaccaga ccggcatccc acattttacg ccagcgtgca tcggcgattt ttccgcgata
    10381 atcaaaggcc agcgggcgga agaacagcga gcacagcacc aggatcatcg ccacataaaa
    10441 gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc cacctgcgag
    10501 gatcaaccag acctgattgc cttcccagtg cgcaccaacg ctattaatca ctatccggcg
    10561 ttcatcatca tcacgcgcca ccagcggcag caaacagcca atccccatgt caaatccgtc
    10621 ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga agcgcaatgt
    10681 ttcataatca aacatgattt tctcctttac ccctgttgct gcgtcggttg ttcactctgc
    10741 atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc gattaagaac
    10801 agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt ggttaaagcg
    10861 gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg accaaactcg
    10921 gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca gagcgccatt
    10981 ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag cgcaatcagc
    11041 atcaccagta gcagcaggga accacagccc accatgatgc ggaaactcca gaataccggc
    11101 gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt gacatgattc
    11161 atatccggcg catagcggga gagcaacatg ccgtagccca ggtcgccttc cagctcccgg
    11221 aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat taacagccag
    11281 gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattctt cagacccggc
    11341 acgggtttat ctaatgagtg agtggcgagg atccctaaca gcacgggaat tttgatggca
    11401 aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg tgcaggagca
    11461 ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac ttgcgcgact
    11521 tcatacgcag aactgtctcc gagttgcagg gtaccgataa tcgccagagt accgaagacg
    11581 gaaccgatgg caaacgagcg taatgcgaca tcgcgctccc gtccgcgcag taaataccag
    11641 gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac ggtgtgcaca
    11701 aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat ctccatacgc
    11761 agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt ggcattcaat
    11821 atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag caggtgctgg
    11881 tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga ttcgaggaag
    11941 aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac atagttggaa
    12001 tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc cacgccaaga
    12061 gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta gattgttttg
    12121 ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc cagggtaagg
    12181 ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga taaatcaatg
    12241 acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgtta atggtagatc
    12301 tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat attcataagc
    12361 ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc actgacatac
    12421 ctcactaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca ccacttccgg
    12481 tattgggcag atttcataac tttccagtaa cgggcctttt aaggtgtccg tacagcgtaa
    12541 atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc caatggtacg
    12601 aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag cctcgctgat
    12661 gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta catgcgccag
    12721 taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg gtaacagcga
    12781 atctgtcggc aagagatttt gcgttgccgc ctgccacaac gccagcggcg cgcagccagc
    12841 ctccagtttg tcctccagca acttttcgcc gcgacgtctg cgcacccgcc ataatccgca
    12901 gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc gcactgacac
    12961 ttcaccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga gatttaacag
    13021 atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat gttgtaactc
    13081 gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg ccatactcgg
    13141 ttcatcattc acctgacagg tgatcggcag tggattcatg ctgaaactgt catcgttcgg
    13201 ttgcgttctt ggccccagca gatggaaaaa agtttcgctc atgaggcacg ctcctgctgt
    13261 ggttcgacaa gcccacgcat caggtttatc agttccgccc acgggtgaat accattcagc
    13321 acgccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa gacgccaaag
    13381 cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca tgtgtagtcg
    13441 ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc tggcgtgcgt
    13501 ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca gtcgtcaaga
    13561 cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa cgcatcaaat
    13621 ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc aagcgaacgc
    13681 cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca ttagccggtt
    13741 gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct gcgggcagtt
    13801 gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg ggttgcagac
    13861 cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc agcacttcgg
    13921 agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga atacgttctc
    13981 cggcataggt tcgcagcgtt cctggttcca gcccgtagtc aatggcatcg agaatcaaca
    14041 gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg ccatcgacaa
    14101 tctccacaga ctcgggccag tggtaatggg catacaaccg ttccgccacc cgcacgccga
    14161 aaccttcatc ggcccacagc aggttgccca gccccattac caccacgcgt tgctcgctca
    14221 tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt tgaccatggt
    14281 ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga catgaccgat
    14341 cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt ccattgagtt
    14401 gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa tagcgtactg
    14461 gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca tcaggaaata
    14521 gccaaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg gacgttttgc
    14581 cagaaacaga taccagcgga tttcatacca cacgccctgc caccagcttt tacgccatac
    14641 tggcacgata aacagctcgc gggagtatcg attgccaaca aaagcccagt agatccgcat
    14701 cagcaagacc acggtaaaaa ccatcccggc gctgaagtga attaacctga tgtagcccat
    14761 atagaacaga tacgtcgcct cgccgctgac ggaaggtagc ggtttgccga taaagtaacc
    14821 ggtgaccatc aacaccgcca tgcataacac cgtcagccag tgccagatac gcactggcgc
    14881 ttcaaagaca tagtggctga caacgttgtc gcttttctgt tgcatgatga ttctccttcg
    14941 ctgttaacgc acctgcacgg agatcagctc gctaccgtcg tcgcccagca cgtgtgttga
    15001 acaggcgagg cacggatcaa agctgtgcag agtacgcagg atctccagcg gttgctcggg
    15061 gatcgccatt ttggtgttca tcagcgccgc ttcataagcg ccaatctgcc ctttgggatc
    15121 gcgcgggctg gcgttccagg tggtcggcac cacgcactgg tagagatcaa tcttgccatc
    15181 gcgaatggcg gcccagtggc ctaacgcccc gcgcggcgct tcggtaaaac cgacaccacg
    15241 gcactctgtc ggccaggttg tgggttccca tttttccgtg gaggcggtgg caagattgcc
    15301 gtttttcagg ttagtcatca gcttgttgaa gaaatactgc aacttacctg cggcccactg
    15361 tgcttcgtgc gcgcggcaca aaatgcggcc caacgttgac tggataccgg aaagcggcag
    15421 gttcagcgcc gacatcatgc gatcgaccga ctcaacggtc gcagcatcgc ctttgtgata
    15481 agcgattaac gtgcgtgcca gcggccccac ttccatcgcg ttaccgcgcc agcgcggcgc
    15541 tttgatccac gagtagcgtt cctgttcatt cagctgctga atgttggtat cgctgccttt
    15601 gacatcgccg gggttgtacc acgggtcggt gatgccatcg aacggatgac gcccgacctg
    15661 atcgttggga taacgatacc aggcgtgatc gacaaactcc tgcacctgct gcggatcaac
    15721 caaatccact ggcagcacat tgttgaagtc gccgttaatc accgcgccgc caggcatcag
    15781 cagacttttc tcaccaaagt cgttggcaat atccgggaat gcgccgtagc tgaggacaca
    15841 tttatcagaa agacccgtgc cgatttcgct ccacggtttg ttgaactgac cgatggctaa
    15901 ggcgtcgggg atcatcacgt tgttaatgaa gtctgccgtg cgggtgataa tcgactgcac
    15961 caggttcagg cgttccatat tgaccgcccc gaccgcgccg ctttcgtcaa tgttaatggc
    16021 gcaaggcatc ccgccgacaa tccagtttgg atgcgggttt ttaccgccaa agaccgcgtg
    16081 gattttgaca atttcacgct ggaaatcgag agcttcgaga tagtgggcaa agcccatcag
    16141 gttggcttct ggcggcaact tgtactgcgg gtgcccccag tagccattgc ggaagatccc
    16201 caactgcccg ccttcaacga atttcttcag gcggttttgt acgtcgaaga aatagccagg
    16261 ggatgatttc ggccaagagg agagactttg cgccagttcg gaggttttcc gcgggtcggc
    16321 tttcagcgca tccaacacat cgatccagtc catcccggca agctgataga agtgcaccag
    16381 atgatcgtgg caccagagcg ttgccagcat aatgttgcgg atgatattgg cgttgtccgg
    16441 cactttaata ccgatggcat cttcgatggc gtaaaccgaa gccagagcgt gtacgccagt
    16501 acagacgccg cagatacgtt caacgaacgc ccacgcatcg cgcgggtcgc gcccttgcag
    16561 gatgatctcc agcccgcgaa acatggtgcc gcaggagacg gcattggtga ttacattctg
    16621 atcgttaata ttcacttcgc agcgcatgtg gccttcaatg cgcgtaatcg ggtcgaccac
    16681 caggcggcgt ccggcattat tgatggtgta tccctgagtt tcgtactgag tgctcatgcc
    16741 tgtttatcct cattgcctgg ctgatgttcg gtttctgtag gttgctgatt atgacgtctg
    16801 cgctggtcaa cggcgctggc gactgcgtgc acaccaacag ccgctgccac cacaccaagc
    16861 gcggttaaac cgacggtatc ggcggtggaa tgagtaccca tttgtggaat atcgaccacg
    16921 cggctgtaga acgaaccgcg atcccagaaa ccattttccg cacagcccag gcagccgtga
    16981 ccagactgga ttgggaaaga aacgccatca ttccagcgtg tggaggaaca ggcgttatag
    17041 gtggtaggcc ctttgcagcc cattttgtac aggcaataac ctttgcgggc agcgtcatca
    17101 tcccaactct ggacgaactc tccggcgtcg aagtgagcgc ggcgatagca tttatcgtgg
    17161 atacgctgac cataaaacat cagtggacga cccattctgt cgacatctgg caagcgatca
    17221 aaggtcacca tgtaagtaat gatggcgctc atcacatccg ggatcggcgg gcagccaggt
    17281 actttgataa tgggtttgtc ggtgatgact ttgtcgatag acgttgcctg tgtcggatta
    17341 ggtcgcgcgg cctgcacgca gccccaggac gcacaggttc cccaggcgat aatcgcgctg
    17401 gctccggcag cggcacgttt gagtttctca ataaacggtc gaccgctgct gatacagaac
    17461 atcccctgct cgcccagcgg cggattacct tctactgcga ggatatattt gccattgtat
    17521 tgcgtgatga tgtcttcaaa gacttcttcc gcctgggttc cggcggcagc catcaaagta
    17581 tcgtcgtaat cgagggaaat cagggaaagg atgacgtcct ttgccagcgg gtgagcggag
    17641 cggataaaag attcggtaca gcaggtgcat tccagaccgt ggatccatac caccggaatg
    17701 cgcggtttgt tctccagcgc ccaggcaatc tttggtgcca ttcccgcgcc taatcccagc
    17761 gacgtggcag ccagactaca atatttgaga aagctgcgcc gggtaacgcc ctgacgccgc
    17821 atggcctggt aaaatgtttc ctcgttattc atatcgcacg tctcttctcc ttgcgacacc
    17881 ggcaggacac cgggcgctta tggtcggcaa gcgacgtccc tgtagcaagg caaaccatcg
    17941 cttcacgtga gatgctgaaa acgaaagctc atccttctgc actggcgcac gtcgccagaa
    18001 agtattgtta ataaagcgta gtgaaacttt tgcacaaaac aatacaaact gtgtggattt
    18061 atcttttagc gataaaaatg gacctatttt tctttttgcc gggcggtggg atgtttagcc
    18121 agttgctaaa cgagtaaaag agaaggaatt tatggtgatg aaatgatcaa tctgctttta
    18181 tttcacgcta tcaatgcact tttggctgtt tttcaggcaa acaaacagat taggggttta
    18241 cacgccgcat cgggatgttt atagtgcgcg tcattccgga agtgtggccg agcggttgaa
    18301 ggcaccggtc ttgaaaaccg gcgacccgaa agggttccag agttcgaatc tctgcgcttc
    18361 cgccaaataa tcaaggggtt accaaatggt agcccctttg cttttttggc tcttggtata
    18421 aacttggtat accaattggt atatttttat tcggatgaga ttatcggtca tgagcggaac
    18481 ctcaggttcc tcgtctgatg ccgctcttgc aacacgtt
//
LOCUS       DBFIFS010000072        18405 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.072, whole genome shotgun sequence.
ACCESSION   DBFIFS010000072 DBFIFS010000000
VERSION     DBFIFS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.072"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            358..702
                     /locus_tag="N7A07_002270"
     CDS             358..702
                     /locus_tag="N7A07_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476927.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            780..971
                     /locus_tag="N7A07_002271"
     CDS             780..971
                     /locus_tag="N7A07_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2476928.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(952..2130)
                     /locus_tag="N7A07_002272"
     CDS             complement(952..2130)
                     /locus_tag="N7A07_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2476929.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            2312..3736
                     /gene="sbcB"
                     /locus_tag="N7A07_002273"
     CDS             2312..3736
                     /gene="sbcB"
                     /locus_tag="N7A07_002273"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU2476930.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(3779..4006)
                     /gene="tsuB"
                     /locus_tag="N7A07_002274"
     CDS             complement(3779..4006)
                     /gene="tsuB"
                     /locus_tag="N7A07_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU2476931.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(4020..5078)
                     /gene="tsuA"
                     /locus_tag="N7A07_002275"
     CDS             complement(4020..5078)
                     /gene="tsuA"
                     /locus_tag="N7A07_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU2476932.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(5257..6615)
                     /gene="plaP"
                     /locus_tag="N7A07_002276"
     CDS             complement(5257..6615)
                     /gene="plaP"
                     /locus_tag="N7A07_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU2476933.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(6605..6667)
                     /gene="yoeI"
                     /locus_tag="N7A07_002277"
     CDS             complement(6605..6667)
                     /gene="yoeI"
                     /locus_tag="N7A07_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU2476934.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(6882..7811)
                     /gene="yeeY"
                     /locus_tag="N7A07_002278"
     CDS             complement(6882..7811)
                     /gene="yeeY"
                     /locus_tag="N7A07_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2476935.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(7857..8681)
                     /gene="yeeZ"
                     /locus_tag="N7A07_002279"
     CDS             complement(7857..8681)
                     /gene="yeeZ"
                     /locus_tag="N7A07_002279"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2476936.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            9107..9157
                     /gene="hisL"
                     /locus_tag="N7A07_002280"
     CDS             9107..9157
                     /gene="hisL"
                     /locus_tag="N7A07_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU2476937.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    9133..9256
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            9303..10202
                     /gene="hisG"
                     /locus_tag="N7A07_002281"
     CDS             9303..10202
                     /gene="hisG"
                     /locus_tag="N7A07_002281"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU2476938.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            10208..11512
                     /gene="hisD"
                     /locus_tag="N7A07_002282"
     CDS             10208..11512
                     /gene="hisD"
                     /locus_tag="N7A07_002282"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU2476939.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            11509..12579
                     /gene="hisC"
                     /locus_tag="N7A07_002283"
     CDS             11509..12579
                     /gene="hisC"
                     /locus_tag="N7A07_002283"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU2476940.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            12579..13646
                     /gene="hisB"
                     /locus_tag="N7A07_002284"
     CDS             12579..13646
                     /gene="hisB"
                     /locus_tag="N7A07_002284"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU2476941.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            13646..14236
                     /gene="hisH"
                     /locus_tag="N7A07_002285"
     CDS             13646..14236
                     /gene="hisH"
                     /locus_tag="N7A07_002285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU2476942.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            14236..14973
                     /gene="hisA"
                     /locus_tag="N7A07_002286"
     CDS             14236..14973
                     /gene="hisA"
                     /locus_tag="N7A07_002286"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU2476943.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            14955..15731
                     /gene="hisF"
                     /locus_tag="N7A07_002287"
     CDS             14955..15731
                     /gene="hisF"
                     /locus_tag="N7A07_002287"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU2476944.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            15725..16336
                     /gene="hisIE"
                     /locus_tag="N7A07_002288"
     CDS             15725..16336
                     /gene="hisIE"
                     /locus_tag="N7A07_002288"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU2476945.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(16433..17410)
                     /gene="wzzB"
                     /locus_tag="N7A07_002289"
     CDS             complement(16433..17410)
                     /gene="wzzB"
                     /locus_tag="N7A07_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU2476946.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIESSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(17556..>18405)
                     /locus_tag="N7A07_002290"
     CDS             complement(17556..>18405)
                     /locus_tag="N7A07_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGU2476947.1"
                     /translation="INPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALY
                     DNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRV
                     AYFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQ
                     SVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKR
                     IKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTR
                     DLFGSD"
BASE COUNT         4733 a         4741 c         4604 g         4327 t
ORIGIN      
        1 agaaatacga tttcgatatc tgggtattct ctccatccag aggtgttctt gacgggctac
       61 agtgggatgg ctcgatgttt actgatgatg aatatcagtt cgttattcat gacgccacgc
      121 attggatgag aaaagtatat ccagcagcac cgcagcagga tggtgaatga tgccgccagt
      181 taaagttgtg attatcactt tggtgatgat agtgattgcg agaatcatgt ctggtgaaat
      241 tgtgtggata tggtaatggc taaggcagca gcagagcgca actaacaatc ctcgcactcg
      301 cggggatttc ttttatctga actcgctacg gcgagttttg ttttatggag acaagaaatg
      361 tcagatttgg ctatgaaggt tttgaaatgg caatcaactg gcgatgtcgg catcagtagc
      421 gcaactcttg cctcaatcgc atgtggcctg aaaaagaata tctatggtca tggcttcggt
      481 gctccacatg acgcagccga tttcagacga tgcgttgcac ttgttgagca gattccagaa
      541 atcagagatt cattcgacaa ggttgcaaag cgcgttccgg cattcaaagg aatcctcaac
      601 gaatgggatt ccctcgttgc tctgttgaag tctgaaatga agatacacgg aaacaaagca
      661 ccagagactt acagaagaat cagcgagcta cgcaaggact aacgcctcac actcgatgag
      721 gcctgttcat atctgataga gccgctatat ggcggtttct ttttgcctgg agaattaaga
      781 tgaccgatac cagcctgatt cctgagaaag aagtgatgaa caagctcggt gtttcatcac
      841 gtcagacaat ctggaactat accaaacggc acggatttcc gaagccagtc agaacccacc
      901 ccaaatcata ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga
      961 aatgctcctg atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc
     1021 ctctatccag tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat
     1081 tcgctcagta acatgaggct cgacattcat ctcggacaga cgtgtaacaa gcgttcttct
     1141 gaaatcatgt gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg
     1201 gttagctgca gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt
     1261 gtacgttgtc atggcctttt cgagaaaagg cttaatttga tcgaagattg gacggcggat
     1321 aacattaccc atcttgctat gctctttcgg tacggtccat acgttgtcca gcaggtcaaa
     1381 ttctgtcttt gttgccagcc taagctctga gagcctcgcc ccccacagca taagcatctg
     1441 atgaaggagc ttatttgacg tagacgcacg gcttctttca atagcaagcc agatcttagc
     1501 cagttcgtga tacgacagta cccgatcccc tacctcagcg cgggagccga agtcccttgg
     1561 ttggatgctc ataattgcgc aactatctat caactgacgc cgcatgcacc aactaattgc
     1621 tgatcttagt tgacttagca cctgccttgc tcggcgtgga ttatcttttt cttcttcggt
     1681 aagcaggtca acccattgct taaccgtgat agaagatgcc ggacgattag ggaaggcgtc
     1741 atgcatgcgc ttcataaccg ttgatcggta aagtgcttgg gtcttttctc tgagagttgt
     1801 agagacgtag ttgtcgaacc agtagtcgag acactgggcg accgtcatgg agttctccac
     1861 cttctcttca aaataagtgc gtggatccgt tcctgagaaa tagagctttc gcaagtcagc
     1921 agtgatctgt ctggcatcct tcaaagacag ggatgggtat cgaccaagcc caagtcgatt
     1981 aggcttgcca tgccagcgat agcggtactg gaactggatg acccccttcg gtgaaattcg
     2041 tacgctgagg ccatcggcat cagccacttc ttgtgggcca gaatatggtt taccataaat
     2101 agtacgcagt tttgtgtcgc ttatagccat aaataatatt tggtacgctc agaaataatg
     2161 gtttggtaca catctggtac acaatatcac atggacaaca gcgcacagta aacaactata
     2221 tttgacgttc gtagacatac tcaggtgaaa aaagggttgt ttttcgagtt ataacagaca
     2281 gttaattaac tacattggac aatgctagac aatgacagac acagataagc aacctacctt
     2341 cctctttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt
     2401 cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg
     2461 caagcccgcg gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc
     2521 gcaggaagct cgggcgaaag gagaaaacga agccgcattt gccgcccgta ttcactcgct
     2581 ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt
     2641 cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga
     2701 taactcgcgc tgggatttac tggatgttat gcgtgcctgc tatgccctgc gcccggaagg
     2761 aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa
     2821 agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat
     2881 tgcgatggcg aaactggtaa aaacgcgtca accacgcctg tttgattatc tctttaccca
     2941 tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggttca
     3001 cgtttccgga atgtttgggg catggcgcgg caataccagc tgggtggcac cgctggcgtg
     3061 gcatcctgaa aatcgcaatg ccgtaattat ggtggatttg gcaggagata tttcgccatt
     3121 actggaactg gatagcgaca cattgcgcga gcgtttatat accgcaaaaa ccgatcttgg
     3181 cgataacgcc gccgttccgg ttaaactggt acatatcaat aaatgtccgg tgctggccca
     3241 ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct
     3301 cgataacctg aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tggcgatatt
     3361 cgcggaagcc gaaccgttta cgccttcaga taacgtggat gcacagcttt ataatggctt
     3421 tttcagtgac gccgatcgtg cagcaatgaa aattgtgctt gaaaccgagc cgcgtaattt
     3481 accggcactg gatatcactt ttgttgataa acggattgaa aagctgttgt tcaattatcg
     3541 ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg
     3601 ccgccaggtc ttcacgccag agtttttaca aggctatgca gaagagattc agatgctggc
     3661 gcagcagtat gccgacgata aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc
     3721 ggaagagatt gtctaatgtg aagcctccgc cgctggtacg gcggaggaca atgttgagtc
     3781 aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg
     3841 catgcccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg caatcaaact
     3901 caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg
     3961 gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact
     4021 taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag
     4081 gacgcagtcc agactccgag aatcataaat acaaggccaa cccagccctg ccaggtcatc
     4141 attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag
     4201 cccatcagta caccacctag cccgctacgt actgttgttt gtgcatcagc tgcgcgaacg
     4261 cggaactcac ggctcgcttt ggctgcaata aatgccccca cgaagatccc taacaccagg
     4321 aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc
     4381 gttggggaag tgatgcctaa cccaaacata cgtccggttg cttcactcag gggccaggct
     4441 aaaagcgcaa tcaaaccgat gagtacagct gtgacaaagg gatgccagcg tttttcaaac
     4501 agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc
     4561 ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac
     4621 acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt
     4681 tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa
     4741 agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat
     4801 ccacccgcca gaacaattcc cagcccgaag agatacccac ctataacagt accaagccac
     4861 gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg
     4921 ctttgtaccg aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac
     4981 atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga
     5041 gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg
     5101 ttaatgggga aattattcat gactggtgga atgcagacca ataaccaaat tctctaaatt
     5161 agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc ggagccaaat
     5221 gttcattcga ctccggttta ttattagaaa tgatgattat gctacgtctt cgtactgcgg
     5281 aaccgggtta cggaagctct tggtgacgca agccaggtaa atcagaccga tagcagccca
     5341 gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag
     5401 cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct tattacgctt
     5461 ctcacggatc cagaactgcg agatgaccga caggttaacg aaggtgaacg ccaccaacgc
     5521 accaaagttg atcagcgccg tagccattac caggtcgaag ttgattgcca gcagggcaat
     5581 cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa
     5641 gaaacttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc
     5701 gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg caccgacctg
     5761 gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa
     5821 gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc
     5881 gccgatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc
     5941 ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc
     6001 ggtaatcatc gggataacat gcgcatcgcc agaccagaat ggacgagtgc tcgccagcgt
     6061 accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag
     6121 cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag
     6181 gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg ggatggaagg
     6241 caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata
     6301 gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc
     6361 gtaagtatat gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc
     6421 cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag
     6481 gcctgaaacg ataccaaaag tatcaaacag cgtcatcggc tgcatatagg caagacccat
     6541 cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt
     6601 aacgttatgc gacatggtca ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt
     6661 gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc
     6721 ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct
     6781 gatgaaacgt cggtttttag tcattttttg acagcggcgc attgtgccta aatggggggg
     6841 aaatgacaag agaatgagag gcttgtcata ataatttttc tttaaatggc tgattttccg
     6901 tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc atcccgccac
     6961 cagccgctgg caagtcctgc ggtctgttca ttccagccaa tccagacggg ttcaaagaga
     7021 gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg ttgcgccaga
     7081 taacggggta aatagccgca gccgaggccg ctaatttgca gctccagctt ggttttaaaa
     7141 tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc
     7201 tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa tgatacgacg gtttaatggt
     7261 tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc cagatcgccc
     7321 aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacaataata
     7381 tccgctcgcc cctgagtcag ggcatcccat gaaccaccga gtacgccatt gataaacttc
     7441 aggcgcgtca cgttatgatg ttgataaaaa gcttcaatga gtggcgcaag cagagaaaaa
     7501 ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg cagtttgatc
     7561 gcctgctttt ccagttctcg cacggtatgc agaacttccc gccccttctc taataacatt
     7621 tttccggtac gggtgaattt agcgcggtga ccgctacgat caagcaattg aatattaagg
     7681 tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta gagtttagcc
     7741 gacgccgccg caaaactgcc ttctttttct aaggcatcga ggatcatcaa cacatccagc
     7801 agtggtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt gatcgtttac
     7861 tccagcggca ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc
     7921 cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa ttgcggaggt
     7981 tccagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg gtgagctggc
     8041 gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt gatagcgcca
     8101 ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggcgc ggtttttccg
     8161 gcaaagaagc gtccgggatg acgccccggt ccgaccaggc ccgcaagacg cagaatatcg
     8221 accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac tcgtccactg
     8281 ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca
     8341 gacgttgaac tggtaaaaat aattcgggga atacgatggg ccagcgcgct atccactaac
     8401 tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgggc cggaagcgta
     8461 atgaccagcg catcggcatc catcagggca tccagatcgt cagaatcgca aactaactca
     8521 ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc gacaccatct
     8581 tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat cgccagcggc
     8641 atgcctaacc accctaaccc gacaattgcg acctttttca tccgttatct cctgatacac
     8701 cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta tgaatgaaat
     8761 cgaaactgtt agcaaatata ccattgcctg atgcgctccg cttattagtc ctacaaaaca
     8821 tcaatatatt gaatttaggc aacatttgta gaccagataa ggcattcacg ccgcatccgg
     8881 caggaacaaa gcccacttta gttacagaac acaatgttct tgagggtttt aatgacatat
     8941 tttcacgatt acttttttaa cctagtcact cttagccccc tcctttcccc gctcattcat
     9001 taaacaaatc catcgccata aaatatataa aaaagccctt gctttctaac gtgaaagtgg
     9061 tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga cacgcgttca
     9121 atttaaacag caccatcatc accatcatcc tgactagtct ttcaggcgat gtgtgctgga
     9181 agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga agatcacctt
     9241 ccgggggctt ttttattgcg cggttgataa cggttcagac aggtttaaag aggaataaga
     9301 aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt ttaagtgatg
     9361 actctcgcga attgctggcg cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga
     9421 tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgac gacattcccg
     9481 gtctggtaat ggacggcgtg gtagatcttg ggattatcgg cgaaaacgtg ctggaagaag
     9541 agctgcttaa ccgccgcgcc cagggtgaag atccacgcta ctttaccctg cgtcgtctgg
     9601 atttcggcgg ctgccgtctt tcgctggcaa cgccggttga tgatgcctgg gacggcccgc
     9661 tctccttaaa cggtaaacgt atcgccacct cttatcctca cctgctcaag cgttatctcg
     9721 accagaaagg catctctttt aaatcctgct tactgaacgg ttctgttgaa gtcgccccgc
     9781 gtgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg ctggaagcaa
     9841 acggcctgcg cgaagtcgaa gttatctatc gctcgaaagc ctgcctgata caacgcgatg
     9901 gcgaaatgga agaatccaaa cagcaactga tcgacaagct gctgacccgt attcagggtg
     9961 tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgacccaa cgtctggatg
    10021 aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat tctgccgctg gcgggtgatc
    10081 aacagcgcgt agcgatgcac atggtcagta gcgaaaccct gttctgggaa acgatggaaa
    10141 aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc gattgagaag atgatggagt
    10201 gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcaa agcaacaacg
    10261 ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca ctgttaacga
    10321 tattctcgat agcgtgaaag cacgcggtga tgacgccctg cgggaatata gcgcgaagtt
    10381 tgataaaacc acggttaccg cactgaaggt gtctgctgag gaaattgccg ccgccagcga
    10441 acgcctgagc gacgagctaa aacaggcgat ggcggtggca gtaaagaata ttgaaacctt
    10501 ccacactgcg caaaaactgc cgccggtaga tgtagaaacg cagccaggcg tgcgttgcca
    10561 gcaagtcacg cgcccggtag cttcagttgg gttgtatatt cctggcggct ccgccccgct
    10621 cttctcaacg gtattaatgc tggcaactcc ggcgcgtatt gcgggctgta aaaaagtggt
    10681 gttgtgctca ccgccgccga ttgccgatga gatcctttat gcggcgcagc tgtgcggtgt
    10741 gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc gcgctggcgt ttggtacgga
    10801 atctgtgccg aaagtggaca aaatcttcgg gccgggtaac gcctttgtca ccgaggcaaa
    10861 acgtcaggtg agccagcgtc tggacggtgc ggcgatcgat atgcccgcag gcccgtcgga
    10921 agtgttggtc attgccgaca gcggcgcaac gccggatttc gtggcttctg atttgctttc
    10981 tcaggctgaa cacggcccgg actcacaggt gattttactg acgcctgacg ctgatatggc
    11041 gcatcaagtt gccgaagccg tcgaacgcca gttagcagaa ctgccgcgtg ccgaaaccgc
    11101 ccgccaggca ctgaacgcca gccgcctgat cgtgactaaa gatttagcgc agtgcgtgga
    11161 gatctccaac cagtacggcc cggagcacct gatcattcag acccgcaacg cccgcgaact
    11221 ggtcgatggc atcaccagcg ccggttcggt atttcttggt gactggtcac cggaatcggc
    11281 aggtgattac gcctccggaa ccaaccatgt tctaccgact tacggctaca ccgccacctg
    11341 ttccagcctc ggactggcgg atttccagaa gcggatgacc gtgcaggaac tgtcgaaagt
    11401 aggtttctcc gcgctggctt cgaccattga aacactggcc gccgccgagc gcctgaccgc
    11461 ccacaaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat gagcaccgtg
    11521 actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca gtcggcgcgt
    11581 cgtctgggcg gtaatggcga cgtctggctg aacgccaacg aatatcccac agccgtggag
    11641 tttcagctta ctcagcaaac gctcaaccgc tacccggaat gccagccgaa agcggtgatt
    11701 gaaaattacg cgcaatatgc aggcgtaaaa ccggaacagg tgctggtcag ccgtggcgcg
    11761 gacgaaggta ttgaactact gattcgcgct ttttgcgaac cgggtaaaga cgccatcctc
    11821 tactgcccgc caacgtacgg catgtacagc gtcagcgctg aaaccattgg cgtcgagtgc
    11881 cgcacagtgc cgacgctgga aaactggcaa ctggacttac agggcatttc cgacaagctg
    11941 gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc caaccgggca actgatcaat
    12001 ccgcaggatt ttcgcaccct gctggagtta acgcgcggta aggcgattgt ggttgccgat
    12061 gaagcctata tcgagttttg cccgcaggca tcgctggctg gctggctggc ggaatatccg
    12121 cacctggcta ttttgcgcac actgtcgaaa gcttttgctc tggcgggcct tcgttgcgga
    12181 tttacgctgg caaacgaaga agtcatcaac ctgctgatga aagtgattgc cccctacccg
    12241 ctctcgacgc cggttgccga cattgcggcc caggcgttaa gcccgcaggg aattatcgcc
    12301 atgcgcgaac gggtggcgca aattattgca gaacgcgaat acctgattgc cgcactgaaa
    12361 gagatcccct gcgtggagca ggtttttgac tctgaaacca actacattct ggcgcgcttt
    12421 aaagcctcca gtgcagtgtt taaatctttg tgggatcagg gcattatctt acgtgatcag
    12481 aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg tgaagaaagc
    12541 cagcgcgtca ttgacgcctt acgtgcggag caagtttgat gagtcagaag tatcttttta
    12601 tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg gatcgttttg
    12661 acaaactcgc ctttgaaccg ggcgtgatcc cgcaactgct gaagctgcaa aaagcgggct
    12721 acaaactggt gatgatcact aatcaggatg gtctgggaac acaaagtttc ccgcaggcgg
    12781 atttcgatgg cccgcacaac ctgatgatgc agatattcac ctcgcaaggc gtacagtttg
    12841 atgaagtgct gatttgtccg cacctgcccg ccgatgagtg cgactgccgt aaaccgaaag
    12901 taaaactggt ggagcgttat ctcgctgagc aagcgatgga tcgcgccaac agttatgtga
    12961 ttggcgatcg cgcgaccgat attcaactcg ctgaaaacat gggcattaat ggtttacgct
    13021 acgaccgcga aaccctgaac tggccaatga ttggcgagca actcaccaga cgtgaccgtt
    13081 acgctcacgt agtgcgtaat accaaagaga cgcagattga cgttcaggtg tggctggatc
    13141 gtgaaggtgg cagcaagatt aataccggcg ttggcttctt tgatcacatg ctggatcaga
    13201 tcgccaccca cggcggtttc cgtatggaaa tcaacgtcaa aggcgacctc tatatcgacg
    13261 atcaccacac cgtcgaagat accggcctgg cgctgggcga agcgttaaaa attgccctcg
    13321 gcgataaacg cggtatttgc cgctttggtt ttgtgctgcc gatggacgaa tgccttgccc
    13381 gctgcgcgct ggatatctct ggtcgcccgc acctggaata taaagccgag tttacctacc
    13441 aacgcgtggg cgatctcagc accgagatga tcgagcactt cttccgttcg ctctcttaca
    13501 ccatgggcgt gacgctccac ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaga
    13561 gcctgttcaa agcctttggt cggaccctgc gccaggccat ccgcgtggaa ggcgataccc
    13621 tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc cttgatactg gctgcgccaa
    13681 cctgaactcg gtgaagtctg ccattgcgcg tcacggttat gaacccaaag tcagccgtga
    13741 cccggacgtg gtgttgctgg ccgataaact atttttaccc ggcgttggca ctgcacaagc
    13801 agcgatggat caggtgcgtg agcgcgagct gtttgatctc atcaaagcct gtacccaacc
    13861 ggtgctgggc atctgcttag ggatgcaact gctggggcgg cgcagtgaag agagcaacgg
    13921 cgtcgatttg ctgggcatca tcgacgaaga cgtgccgaaa atgaccgact ttggtctgcc
    13981 gctgccgcat atgggctgga atcgcgttta cccgcaggca ggcaaccgcc tgtttcaggg
    14041 gattgaagac ggcgcgtact tttactttgt tcacagctac gcaatgccag tcaatccgtg
    14101 gaccatcgcc cagtgtaatt acggcgaacc attcaccgcg gcggtacaaa aagataactt
    14161 ctacggcgtg cagttccatc cggagcgttc tggtgccgct ggcgctaagt tgctgaaaaa
    14221 cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac tgtggtgcgt
    14281 ctccatcagg gcgattacgg caaacaacgc gattacggta acgacccgct gccgcgctta
    14341 caggattacg ccgcacaagg tgccgaagtg ctgcatttgg tggatctgac cggggcaaaa
    14401 gatccggcaa aacgtcaaat cccgctgatt aaaaccctgg tcgctggcgt taacgttccg
    14461 gtgcaggttg gcggcggcgt gcgtagcgaa gaagacgtgg cggcgttact ggaagctggg
    14521 gttgcgcgcg tggtggttgg ctccactgcg gtgaaatcac cagaaatggt gaaaggctgg
    14581 tttgaacgtt tcggtgccga tgccttagtg ctggcgctgg atgtccgtat tgacgagcaa
    14641 ggcaacaagc aggtggcggt cagcggctgg caagagaatt cgggcgtgtc actggaacaa
    14701 ctggtggaaa cctatctgcc cgtcggcctg aaacatgtgc tgtgtaccga tatctcgcgc
    14761 gacggcacgc tggcaggctc taacgtctct ttatatgaag aagtgtgcgc cagatatccg
    14821 caggtggcat ttcagtcatc cggcggcatt ggcgacatta acgatgttgc tgccctgcgt
    14881 ggcactggcg tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa attcaccgtg
    14941 aaggaggcca tctcatgctg gcaaaacgca taattccatg tctcgacgtt cgtgatggtc
    15001 aggtggtaaa aggcgtacag tttcgcaacc atgaaatcat tggcgatatc gtgccgctgg
    15061 caaaacgcta cgccgaagaa ggtgcagacg aactggtgtt ctacgatatc accgcttcca
    15121 gcgatggccg tgtggtagat aaaagttggg tatctcgcgt ggcggaagtg atcgacattc
    15181 cgttctgcgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt ctttcctttg
    15241 gcgcggataa aatttccatc aactcccctg cgctggcgga cccgacgtta attactcgcc
    15301 tggcggatcg atttggcgtg cagtgtattg tggtgggtat tgatacctgg tacgacgctg
    15361 aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc cgcgtcactc
    15421 agtgggaaac gctcgactgg gtacaggaag tgcaaaaacg cggtgccgga gaaatcgtcc
    15481 tcaacatgat gaatcaggac ggcgtgcgta acggttacga cctcgaacaa ctgaaaaaag
    15541 tgcgtgaagt ttgccacgtc ccgctgattg cttccggtgg cgcgggcacc atggaacact
    15601 tcctcgaagc cttccgcgat gccgacgttg acggcgcgct ggcagcttcc gtattccaca
    15661 aacaaataat caatattggt gaattaaaag cgtacctggc aacacagggc gtggagatca
    15721 ggatatgtta acagaacaac aacgtcgcga actggactgg gaaaaaaccg acggactgat
    15781 gccggtgatt gtgcaacacg cggtatccgg cgaagtgtta atgctgggct atatgaaccc
    15841 ggaagcctta gacaaaacca tcgaaagcgg caaagtcacc ttcttctcgc gcactaaaca
    15901 gcgactgtgg accaaaggcg aaacgtcggg caatttcctc aacgtagtaa atattgcgcc
    15961 ggactgcgac aacgacacgc tactggtact ggcgaatcct atcggaccaa cctgccacaa
    16021 aggcaccagc agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga
    16081 acaattgctc gccgaacgca aatctgccga cccggaaacc tcctacaccg ccaaactgta
    16141 tgccagcggc accaaacgca ttgcgcagaa agtgggcgaa gaaggcgtgg aaaccgcact
    16201 ggccgcgact gtacatgacc gctttgagct gaccaacgag gcgtctgact tgatgtatca
    16261 cctgctggta ttgttgcagg atcaggatct ggatttaaca acggtgattg agaacctgcg
    16321 taaacgtcat cagtgagttg cggggtaagc ggatgcgata ttgttgtcgc atccggcaaa
    16381 aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
    16441 cgttgtaatt acgcagagcg ttacgcccca gcacaatccc cgcgccaacc atgccgccca
    16501 gcagcaccgc cagaatcaag gtgattgcct ttttcggact atcgcgacga ataggtaacg
    16561 tcggtttcat tacatagcgg taagcatgaa tatcaagatc atcaaccttt aagttttcaa
    16621 tatccaacag gttttgacgt gtctgatagt agtttggtga gaacaccaac ggacgggtcg
    16681 cctcatgctt aatcatcgac tccagcgctt cgctccccaa aaggaacaac gtatcttgcg
    16741 tcacatcttc agtctgctga acctgtggct ttgccacctg cgcctgattc gcatactgca
    16801 acgcttcctg aatctgacgg atacgcagct ctttctgctc ctgcgcaact acttcctgcg
    16861 ttcttaaaga gtcctgcaag tttttccgtc ccagagcaat gttgtctttg agatcctttt
    16921 ctagctcttg attcacttta tcatcaactt gctgaatgta ttgggccaac ttcatttgtg
    16981 ctccctctgc agtttgccca acataagaaa cagtcaatgg taattgctgg ttcttaacag
    17041 aagattcgat ggtaagtttt tctggttctt cctgattatc cagcgtttct gctaatgctg
    17101 agaaggcaga actgaagcga ccaatcaacg tctcctgcaa atccgatact ttcggtgcag
    17161 cctgaccata gataacattc atggcattgt tatagccagc aatttgcccc acgtcgggct
    17221 gagtgataat tgctgttgac gtccatttct ccttcgctac tgccaaataa ccaatagcca
    17281 gagcaatagc cacaatgacg gaaatgatga ttgtcatctt gccacgccac aactgcacta
    17341 gtaaatcaat caaatcaatc tgttccgggt catggttttg cccagaaaca ttattatttt
    17401 ctactctcat cattacccta actgacggaa atttccaagt ccgcatagtt tagcgataat
    17461 ccctgtgaat gctataggat aagtgataaa aataatgaat tattgcggag gtaacaaaag
    17521 acaatcaggg cgtaaatagc cctgataaca agatgttagt cgctgccaaa gagatcgcgg
    17581 gtgtagacct tatctgccac atccctaagc tcttctgcca tacggttgga aataatgacg
    17641 tcggcttgtt gtttgaaggt ggcgagatca cgttccaggc gagagttgaa gaatgagtct
    17701 tctttcatca ctggctcgta gatgattact tcaacacctt tcgccttgat acgcttcata
    17761 atcccctgaa tagaagacgc acggaagtta tctgaaccgc tcttcataat cagacgataa
    17821 atacctacca cttgcggctt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg
    17881 cggttagcat cgacaattgc cgagatcagg ttattcggca cagactggta gttcgccagt
    17941 aactgcttgg tatctttcgg caaacaataa ccaccataac caaacgacgg attgttgtaa
    18001 tggttgccaa tacgtgggtc gagacaaacg ccttcgatta tttgacggga attcagacct
    18061 aaactttctg cgtagctatc cagttcgtta aagtacgcca cgcgcatcgc gaggtaggtg
    18121 tttgcaaaaa gtttaatcgc ttctgcttca gtggagtcgg taaacagggt tgggatattt
    18181 tgcttaatcg cgccttcctg taataacgca gcaaaacgtt ctgcgcgttc cgaacgctca
    18241 ccgattacaa tacgtgacgg atgaaggtta tcgtaaagag ctttaccttc gcggagaaat
    18301 tccggagaga aaataatatt ttcagtacga tatttcttat gcatcgcttc ggtaaaacca
    18361 acgggaaccg ttgatttgat gaccataacc gcataaggat ttatc
//
LOCUS       DBFIFS010000073        18209 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.073, whole genome shotgun sequence.
ACCESSION   DBFIFS010000073 DBFIFS010000000
VERSION     DBFIFS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.073"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(63..869)
                     /gene="trpA"
                     /locus_tag="N7A07_002291"
     CDS             complement(63..869)
                     /gene="trpA"
                     /locus_tag="N7A07_002291"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU2476948.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(869..2062)
                     /gene="trpB"
                     /locus_tag="N7A07_002292"
     CDS             complement(869..2062)
                     /gene="trpB"
                     /locus_tag="N7A07_002292"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU2476949.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2074..3435)
                     /gene="trpCF"
                     /locus_tag="N7A07_002293"
     CDS             complement(2074..3435)
                     /gene="trpCF"
                     /locus_tag="N7A07_002293"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU2476950.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3436..5031)
                     /gene="trpD"
                     /locus_tag="N7A07_002294"
     CDS             complement(3436..5031)
                     /gene="trpD"
                     /locus_tag="N7A07_002294"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU2476951.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5031..6593)
                     /gene="trpE"
                     /locus_tag="N7A07_002295"
     CDS             complement(5031..6593)
                     /gene="trpE"
                     /locus_tag="N7A07_002295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU2476952.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6615..6708)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6685..6729)
                     /gene="trpL"
                     /locus_tag="N7A07_002296"
     CDS             complement(6685..6729)
                     /gene="trpL"
                     /locus_tag="N7A07_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU2476953.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6867..7748
                     /gene="rnm"
                     /locus_tag="N7A07_002297"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6867..7748
                     /gene="rnm"
                     /locus_tag="N7A07_002297"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU2476954.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7745..8365
                     /gene="yciO"
                     /locus_tag="N7A07_002298"
     CDS             7745..8365
                     /gene="yciO"
                     /locus_tag="N7A07_002298"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU2476955.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8393..9976
                     /locus_tag="N7A07_002299"
     CDS             8393..9976
                     /locus_tag="N7A07_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGU2476956.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            10189..11061
                     /gene="rluB"
                     /locus_tag="N7A07_002300"
     CDS             10189..11061
                     /gene="rluB"
                     /locus_tag="N7A07_002300"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU2476957.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11101..11691)
                     /gene="cobO"
                     /locus_tag="N7A07_002301"
     CDS             complement(11101..11691)
                     /gene="cobO"
                     /locus_tag="N7A07_002301"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU2476958.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11688..12446)
                     /gene="yciK"
                     /locus_tag="N7A07_002302"
     CDS             complement(11688..12446)
                     /gene="yciK"
                     /locus_tag="N7A07_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU2476959.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12666..13715
                     /gene="sohB"
                     /locus_tag="N7A07_002303"
     CDS             12666..13715
                     /gene="sohB"
                     /locus_tag="N7A07_002303"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU2476960.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13751..14002)
                     /gene="yciN"
                     /locus_tag="N7A07_002304"
     CDS             complement(13751..14002)
                     /gene="yciN"
                     /locus_tag="N7A07_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU2476961.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14382..16979
                     /gene="topA"
                     /locus_tag="N7A07_002305"
     CDS             14382..16979
                     /gene="topA"
                     /locus_tag="N7A07_002305"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU2476962.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17189..18163
                     /gene="cysB"
                     /locus_tag="N7A07_002306"
     CDS             17189..18163
                     /gene="cysB"
                     /locus_tag="N7A07_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU2476963.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4408 a         4547 c         4837 g         4417 t
ORIGIN      
        1 ggacttttcc ggcttcgtta aagaaagtta aaatgccgcc agcggaactg gcggctgtgg
       61 gattaactgc gcgtcgccgc tttcatcggt tgtacaaaag ctttcagtgc cgccagcatt
      121 ttctttggct cattaatatg ttgctcgatg attttaacaa tcgcagaacc agaaatcgcg
      181 cccgcagctc ctgcatcaat cgctgctttt acctgatccg gggcggaaat accaaatccc
      241 tgcaatggag gtgccgcgtt gtactctttc agcttcgcaa ccagatgatt gaggggtaac
      301 gcggcgcggt tttctgcgcc ggtcacgccc gctcgtgaca gcaaatatgt gtaaccacga
      361 ccgtaagagg ctatctggcg cagcaggtcg tcatcggcat ttggcgggca gatgaagata
      421 ggtgcgacat tatgacgcaa cgcggcctgg cggaagggcg cggactcttc aactggcaca
      481 tcggcaacca gcaccgaatc gacgccgact ttctcgcact gggcataaaa ctcatcaatg
      541 cctttgttaa acaccaggtt ggcatacatc agcaggccaa tgggaatggt cgggtgtttc
      601 tggcgaatga gtgccagcat ctcaaagcac tgcgccgggg tcacacctgc cgcaaaggca
      661 cgcagtgtgg cgttttgaat cgtcgggcca tctgccagtg ggtcggagaa ggggatgcct
      721 aactccagcg tgtcagcacc ggcttcaatt agcgtatcga taattttcag cgactgctca
      781 atgcccggat caccgagcgt gacgaaagga acgaatgcgc cttctttgcg ctccttcaac
      841 tgggcaaaca gagattcgta gcgttccatc agatttcccc tcgtgctttc aaaatatcgt
      901 gaacggtgaa gatgtcttta tcgccgcgac cggaaaggtt aaccaccagt agctgctctt
      961 tttccgggtt ttcgcgcatc attttcagcg catgggccag ggcgtgggag gattccagcg
     1021 ccgggataat cccttcgtgc aggcacagcg ttttgaaggc ttccagtgct tcatcatcgg
     1081 taattgacac ataatcagcg cgtccagtgc tgttaagata cgcgtgttgt gggccgacgg
     1141 acggaaaatc gagtccggcg gagatggagt aagattcttc aatctgcccg tcttcggttt
     1201 gcatcatcgg cgctttcata ccgaaataga tacccacgcg accatgtttt agcggtgcgc
     1261 cgtgctcgcc agtttcgata ccgtgaccac caggctccac accaatcagg ccgacgttgg
     1321 tttcgttgat gaaatctgca aacatgccga tggcattcga accgccgcca acacaggcga
     1381 taacggcatc cggcaggcga ccttctcttt ccagaatctg tgctttggtt tcttcgccaa
     1441 tcatccgctg aaactcacgg acaatggtcg gataaggatg tgggccagct gcggtgccca
     1501 gcatatagtg cgcggtttca taactgccgg accagtcgcg cagcgcctcg ttacaggcat
     1561 ctttcagcgt cgcggaaccg ctatgcaccg ggatcacttc cgcacccatt aaacgcatac
     1621 gaaaaacgtt aggcgactgg cgttcaacgt ctttggcccc catataaata cggcatttca
     1681 ggccgagcag ggcgctggca agggccgacg ccacgccatg ctgaccggcc ccggtttcgg
     1741 cgatgatttc ggttttaccc atccgcttcg ccagtaaagc ctgaccgagc acctggttag
     1801 ttttatgcgc gccgccgtgc agcaaatctt cgcgcttcag atacagcgtg gtgttcgtgc
     1861 cagccgtaat gttctggcat ttggtcagcg cggttggacg cccggcatag tttttcagca
     1921 ggtcgttgaa ctgagcctga aattcaggat ctttttgcgc gctgacaaaa gcttcttcca
     1981 gctggcgcag agcaggcatc aggatttgtg gcacgtacat gccgccaaac tcaccaaaat
     2041 aggggttaag taatgttgtc attgttcctt tccttaatat gcgcgcagcg tctggaaaac
     2101 cgaggccaaa agacgtgcgt ctttgatgcc cggttgcgac tctacagcag aattaaaatc
     2161 aagtccggcg cagccggttt gtgccgcttc cacgcagtta tctgcgccta agccccccgc
     2221 cagcagaacg ttgccaagcg attgaccatt taatagtgac cagtcgaaac gttgcccgct
     2281 tccaccctgg ccgttgtcta aaacatattt atcaacgtgc tgaagctcgc gggcgggcag
     2341 ggtttcaccg acgcttaatg ctttccagat ggcaacgtgt gctggcagag cttcacgcag
     2401 cgtatcgata tacagctgat cttcattacc atgcagttgc actgccacca gtgataacac
     2461 cttagctttg tccagcacat cggcaatatc gtgattgcgg aacacgccaa catactgcaa
     2521 cggtgccgca gccatcactt cctgcgcctg ttcaacgttg acgcaacgcg gtgatgtcgc
     2581 aacaaaaatc aacccaccgt aaatcgcgcc cgcgtcgtac gctgctttag catcttgccc
     2641 acgcgtcagg ccacatactt tattctcacc cagcaacacc cgacgcacgg cggcgtgcaa
     2701 atcgtcatgg gccatcaacg ccgaaccaat tagaaaaccg tcagcgaagt ggcttaactc
     2761 gcgcacctga gcgtaagtat tgatgcctga ttcgctgatt accgtcacgt tgtgccccag
     2821 tttcggcgca agctcgcggg tacggttgag atcaatcgac aaatcacgca gatcgcggtt
     2881 gttgatgcca acgacctttg cccccaatgc aatggcgcgc tccagttcct cttcattact
     2941 gacttcggtc agcacaccca tcttcagact gtgggcgacg gcggcaagct ggcgatattg
     3001 ttcgtcatcc agtactgaaa gcattaataa gcaggcatcg gcctggtaat agcgcgccag
     3061 atagatctgg taagggtcga taatgaagtc tttacataaa atcggctgcg gggcgatttg
     3121 gctgacgatg gggaggaaat caaagctccc ctgaaaatat ttctcatcag tcagcaccga
     3181 aattgccgaa gcgtaatgtt tataaatggc ggcaatgcgt gctggatcga aatcatcaca
     3241 gatcacgcct tttgacggcg acgctttctt gcactccaga ataaacgccg tgcgcgcacc
     3301 ctgtagcgca tcataaaaat gtcgcgtgct cggctgaacc tcattctgaa aactggccag
     3361 cggttgctgc tgtttgcggg cttctaccca aatcgccttg tctgcgacga ttttcgctaa
     3421 aacggtttgc atcatttacc ctcgtgccgc cagtgcggtg actctgtcgt aagcggaacc
     3481 actgcgcagt acctcaagaa cggtttgcgc attggcttgc agatcttcat ggccatgcag
     3541 gcgcattaac atggcgacat tcgccgcgac ggctgcttca tgggcggcgt cgcctttacc
     3601 ttgtaacaag cgtgttaaaa tgtcacggtt ttcttccggt gttccgcctg ccagttgctc
     3661 ctggtggtag ggagtcaggc caaaatcttc agcggtcaat tgatagctct taatttcgcc
     3721 gtcatgcagc tcggcaacga ttgtcggcgc gtgtaatgaa acttcatcca tcccgccgct
     3781 gtgcaccacc gccgcgcgtt gataccccag cacgcgtaag gtttcggcaa tcggcagcac
     3841 cagttccgga ctataaacac caattaacgc cagcggcgga tgcgccgggt taatcaatgg
     3901 ccccagcaca ttgaacaggg tgcgggtttt tagttgctgg cgaaccggca ttgcatggcg
     3961 aaatccggtg tgatatttcg gtgcaaagag gaaacataca cctaactcat ccagcgcctg
     4021 gcgcgattta tcggcgttca tatcaagatt aataccgaac gccgccagca gatccgacga
     4081 gccagattta ctggagacgc tacggttgcc gtgtttcgcc actttcagcc cacaggccgc
     4141 ggcgacaaac gcactggcgg tggaaatatt gatgctgttg ctgccgtcac cgccggtgcc
     4201 gacgatatcg gcaaacagat aatccggacg cgggaacggc gcggcgtttt ccagtagcgc
     4261 ggttgctgct ccggcgatct cgttcgggtg ctcgccgcga attttcatgc tcaccagcgc
     4321 cgccgccagg tgttccggtt tcagttcacc acgtaccacc gctgaaaata gctgatgact
     4381 ttcctgctgg ctaagggtct gcgcctgata cagtttttcc agaatcggtt gcagcgtgtt
     4441 ggttggctct agtttctgct gcgcccaggc cagcgtttgt tccagcaggc gagcgccctg
     4501 ggtggtgaga atggattccg ggtggaactg gaatccgcaa acgcgatctg catcgtgacg
     4561 caccgccatc accatgccat taaaatgggc gttgatggtt aaaccggccg gaatattact
     4621 gccaaccagc gagtgataac gcgccaccgg cagcgggttt gttaatccgg caaacatcgc
     4681 ctgaccgtca tgttcaatgc tcgacgcttt accgtgaaga atttcgcccg cctgaccgac
     4741 atagcccccg taagcttcga caatcgcctg atgaccaagg caaatgccaa taataggcag
     4801 cttaccgcgc aggcgggtaa gcagttcagg catacaacca gcttcgctcg gcacaccggg
     4861 tccaggagaa agcatcagca ccggattgct catcgtcgca aggcgttcaa ttaaggtctg
     4921 cgccggaata tggttgcggt aaatcaccac gttatgacca ttgctgcgca actgatctgc
     4981 caggttgtaa gtaaaagagt cgatattatc gagcagcaga atgtcagcca ttagaacgtc
     5041 tcctgtgcat gatgcgcggt ggcaatagcg cgcagtacag cgcgggcttt attacgggtt
     5101 tcgtcggctt ccgactgcgg aacagaatca aggactacgc cagcaccggc ttgcacggta
     5161 gcgataccgt tttccaccag cgccgagcgg atcacaatgc aggtgtcgag atcgccgtgc
     5221 gcggtgaaat aacctaccgc gccgccgtag ctgccgcggc gacgaccttc agcctcggca
     5281 attaactgca tagcgcgtac tttcggcgca ccgcttaacg tccccatatt catacaggcg
     5341 cggtaagcgt gcagggcgtc gagatcgtgg cgcagctcac cgaccacgcg ggagaccagg
     5401 tgcatcacgt aagagtaacg gtcaactttg gtgaggtcgg cgacgtagcg gctgccgggg
     5461 gtgcaaatgc gtgccaggtc attacgggcg agatccacca acatcagatg ttcagaaagc
     5521 tctttatgat cggtacgcat ttccagttca atacggcttt caaggtccct gtcgagcgag
     5581 ccatcggcgc gacgaccgcg tggacgtgtt ccggcaatcg ggtagatctc aatctggcgg
     5641 ctggtggcgt catacttgag cgaactttcc ggcgacgcgc caaacagggt gaaatcatta
     5701 tcctgcataa aaaacatgta cgggctggga ttactctttt tcagcacgta ataggccgcc
     5761 agcggtgacg ggcagggcag agagaaacgg cgagatggca ccacctggaa aatttctccg
     5821 gcgcgaatcg ctttttgcaa caaacgcact acgccaccga actcttcatc gctctggtta
     5881 cattcacaac gcatatgcgg cacggaaacc accggcagcg gtggcgcggt ttcggtcagt
     5941 tgctggcgaa gatcgttcag gcgagcagtg agacgttgtt tttcttcttc attcggagca
     6001 aacaggctgg cctgaatgcg ggtgcttttt ttctgatggt caatcaccat cagagtttca
     6061 gcgagataaa aacagaaatc agggcagtta ttttccgctg acagttgcgg taaatcttca
     6121 aaccccgcca caaggtcata agagaacagg ccgccgaaga acatggcttc tcgttcttcc
     6181 ttcggtacat tcaacagatt ctgcaataag cggaaagcgt caaaaaccga aagggagcat
     6241 aagcgagcgt cttcatccag cagtggacca acagggggga agcgcagcac acggcagttt
     6301 ggtaattgtt cattttccac acccgcaggc agggcgttat ccagtagtgt cagcagggct
     6361 tcgccgttgc cggaaaggga ctggattgtg acagtgtcac ctaaagctgt aatgcgcagc
     6421 gcactgtcta ccagcagcag gctttttaaa tcatctttgc tgtcgatatc tgcggattcc
     6481 agcagcagcg ttgccggacg atccccacac aattggtgaa aaagcgcggt gggattgtcg
     6541 cgataagcgc cttcgcaggt tagcagttcg agagtcggtt tttgtgtttg cattgttatt
     6601 ctctaatttt gttcaaaaaa aagcccgctc attaggcggg ctgggtatct gattgcttta
     6661 cgcatggtga atacactgcc cgtttcagga agtgcgccac caacctttca gtacgaaaat
     6721 tgctttcatt gtcgataccc tttttacgtg aacttgcgta ctagttaact agttcgatga
     6781 ttgattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa
     6841 aacattatcc agaacgggag tgcgccttga gcgacacgaa ttacgcagtg atttacgacc
     6901 tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg gtgcaccgtg
     6961 cagtcgagat gcgcgtcggc accctggcga tcaccgacca tgacaccaca gctgcaatcg
     7021 cgccagcaag agaggaaatt tcacgttcgg gactggcgct gaatcttatt cccggcgtgg
     7081 aaatttccac ggtctgggaa aatcatgaaa ttcatattgt cgggctgaat attgatatta
     7141 ctcatccgct aatgtgtgag ttcctcgcgc agcagacaga acggcgcaat cagcgggcgc
     7201 agcaaattgc cgaaagactt gaaaaagcgc atatacctgg ggcgctggaa ggcgcacaac
     7261 gactggcgca gggcggcgca gtgacgcgcg gtcattttgc gcgtttcctt gttgagtgcg
     7321 gcaaagccag ttcaatggcg gatgtcttta aaaagtatct ggcgcgcggg aaaaccggat
     7381 acgttccgcc acagtggtgt acaatagaac aagctattga tgtcattcat cattctggcg
     7441 gtaaggcggt gctagctcat cctgggcggt acaatcttag tgctaaatgg ctgaaaagat
     7501 tggttgcgca ttttgccgaa cacgacggcg acgcgatgga agtcgcgcag tgccagcaat
     7561 cgcccaatga acgtacccag ctggcgacgc ttgcccgcca gcaccattta tgggcatcac
     7621 aaggatctga ttttcatcag ccatgcccgt ggatcgaact gggtcgtaaa ctctggctgc
     7681 ccgcaggcgt tgaaggcgtc tggcagctat gggaacagcc gcagaacacc acagagaggg
     7741 aattatgagc cagttttttt atattcatcc tgataacccg cagcaacgtc taatcaacca
     7801 ggcggtggag atcgtgcgta aaggcggggt gattgtttat ccaactgatt ccggctatgc
     7861 gctcggctgt aaaattgaag ataaaaacgc catggagcgt atttgtcgta ttcgccagct
     7921 gccggacggt cacaacttta ccctgatgtg tcgcgatctt tctgaactct cgacctattc
     7981 atttgtcgat aacgttgcgt ttcgtttaat gaaaaacaac acgccgggca actatacctt
     8041 catcctgaag gggacgaagg aggtgccacg ccgtctgttg caggaaaaac gcaaaaccat
     8101 cggtatgcgt gtgccttcta acccgattgc gcaggcgttg cttgaagcac tgggcgaacc
     8161 gatgctttcc acttcgctaa tgctgccagg cagcgaattt accgaatccg atcccgaaga
     8221 aatcaaagac cgtctggaaa aacaggtcga tttgattatt cacggcggct atctcgggca
     8281 gaaaccaaca acggttattg atcttaccga tgatacgccg gtcgtggtgc gtgaaggcgt
     8341 aggtgatgtg aagcctttct tataacaaat tgcgttccac ggatggaaga ctatggcagg
     8401 gaagtttcgc tgcattttgc tgttgatagt tgggcttttt ttctcttcgt taagttatgc
     8461 gaaaaacacg gagatgcctt cttatgaaga agggatctcg ctctttgatg ttgaagccac
     8521 tctgcaaccg gatggggtgc tcgacatcaa agaaaatatt cattttcagg cgcgaaatca
     8581 gcagattaag cacggatttt atcgtgattt accacgacta tggatgcagc ctgatgggga
     8641 cgctgcactg ctgaactatc atattgttgg cgtcacccgt gatggtattc ctgaaccctg
     8701 gcatcttgac tggcatatcg ggttaatgag tattgtcgtg ggcgataaac aacgtttctt
     8761 gcctcaaggc gactatcatt atcaaattca ttatcaggtt aaaaatgctt tcctgcgtga
     8821 gggagattca gatctgttaa tctggaacgt gactggtaac cactggccgt ttgaaatcta
     8881 taagacccga ttttcactca agttccctga tatcgcgggt aatccattta gcgaaatcga
     8941 tctctttact ggagaagagg gcgacacata tcgaaatggc cgcatccttg aggacggaag
     9001 aattgaatcc agcgatccgt tttatcgtga agatttcacg gtactctacc gttggcctca
     9061 ctcgttactt agcaatgccc cggctccaca aacgacgaat attttcagcc atcttctttt
     9121 accctccacg tcatcgttgt taatttggtt tccgtgtcta ttcctggttt gtggatggtt
     9181 atatctctgg aagcgcaggc cgcaatttac gtcggtagat gttttacgga tgcagaaatt
     9241 ttatctgccg cgtaaaaagt cttcgtttta ccggcctgat acttttttgc aatggggtgg
     9301 actggcaata ttggcggtca ttctttacgg taacctgagt cctgtaggct gggcaggaat
     9361 gagtctggtg ggcgatatgt ttattatgat ctgctggctt cttccttttt tattttgttc
     9421 ccttgagctt ttgtttgccc gcgatgatga caagccttgc gttaatcgtg taatcatcac
     9481 tttgttttta ccgctgattt gttcaggcgt ggccttttat tctctctata tcaatgtcgg
     9541 agatgtattc ttttactggt atatgccagc gggttatttt agcgctgttt tcctgaccgg
     9601 ttatctcact ggcatggggt atatttttct gccaaagttt acccaaactg ggcagcaacg
     9661 ttatgcccac ggtgaagcta tcgttaacta tctcgcgcgt aaagaggcag caacacacag
     9721 tgggcgtcgg cggaaagggg aaacacggaa actggattac gcgttgctag gttgggctgt
     9781 ctcagcaaac cttggaagag aatgggtagc acgtatcacc ccatcactca cagcggctgt
     9841 tcgcgccccg gaaattgccc gtagtggcgt tttgttctca ttacagatgc acctgagtct
     9901 gggggccaat accagtttat tggggcgaag ttattccggt ggtgctgcgg gtggcggagg
     9961 cggtggtggc tggtaattaa gctgatgtta atcgaaacaa gctttaacca taaagatagt
    10021 gtagtatgtt gcgcctcaaa gccaggccag ttaaaacgtc gagtcgttta cttaaggcct
    10081 gaagagttca aacagacttt actatcagtg agtcagatgt aagttctgac tcactgttgc
    10141 acaaaagcat atcagacgcc tgggaaggcg acaccaaaag gaagctctat gagcgaaaag
    10201 ctacagaaag tgctggcgcg tgccggccac ggttctcgcc gtgaaatcga atctattatt
    10261 gaagccggtc gtgtgagtgt tgatggcaaa attgctaaac tcggcgatcg cgttgaagtt
    10321 acccctggat tgaaaatccg tatcgatggt cacctgattt cggtacgtga gtccgctgaa
    10381 caaatttgtc gcgttctggc ctattataaa ccggaaggtg agttgtgtac ccgtaacgat
    10441 ccggaagggc gtccaacggt gtttgaccgt ctgccaaagc tgcgcggtgc acgctggatt
    10501 gccgtaggtc gtctggacgt taacacctgt ggtctgttgc tgttcaccac cgacggtgaa
    10561 ctggctaacc gtttaatgca tccaagccgt gaagttgaac gtgaatatgc cgtgcgtgta
    10621 tttggtcagg ttgacgacgc gaaactgcgt gatttaagcc gtggtgtgca gctggaagat
    10681 ggtccggcag cctttaaaac catcaagttc agcggcggcg aagggatcaa ccagtggtac
    10741 aacgtgactc tgaccgaagg ccgtaaccgt gaagttcgtc gtctgtggga agcggttggc
    10801 gtgcaggtaa gccgcctgat ccgtgttcgt tacggtgata tcccactgcc gaaaggtctg
    10861 ccacgcggcg gctggacgga gctggatctt gcccagacta actatctgcg cgaactggtg
    10921 gagttgccgc cagaaaccag ctctaaagtc gctgtagaaa aagaccgtcg tcgcatgaag
    10981 gcgaatcaga ttcgtcgtgc ggtgaaacgt cacagtcagg tgagcggcgg acgtcgttct
    11041 ggtgggcgta ataacggtta atcaacgtat gccggacgtc atatccggca tttttacaga
    11101 ttagtaatca atccctatct gcgcttttac gccagcatca aacgcatgtt tcacggggcg
    11161 taactcactt accgtatccg ccagttcgag tatatcccga tgacagcccc gacccgtgat
    11221 aatcaccgtc tgctgatgtg gacgttcatt taacgcctgc aacacttctt ccagtggcaa
    11281 atagtcatac gccaccatat aagtcagctc atcaagcaaa accaaatcca gtgaggaatc
    11341 agcaagcatc cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg
    11401 gttttgtgta tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc
    11461 cagcagattg cgttcgccat taggccaggt gcctttaata aactgcacga cgcccacttt
    11521 ttttccgtga ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc
    11581 attgccggta aagacgatga taataccgcg ttcatcctgg gcctgggcca tacgagcatc
    11641 tactttttct ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt
    11701 cctggtttac ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg
    11761 cccatcagcc agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct
    11821 tcggtcggga aggcgctggc acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg
    11881 acacgcaggc gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat
    11941 ttcgacgctg catatgcacc ccagttggct cgtccctgac gtccaacgct tgatgaagta
    12001 aagaccagtg aaccggcgtc cgatttgagt aataaaggaa gtagtgcctg ggtgagcata
    12061 aaggtggcat taacgttgac ctgtatgacg tcctgccaga cctgtggatt ttgttcgctc
    12121 attggacaaa catcgccgag taatccggca ttatgcaaaa caccatccag acgcggataa
    12181 ttaacggcaa tgcgctgtgc cagttgttgg caatcttcgg acctgcaggt cagcaaatcg
    12241 agaataaacc actgtggctg acgcccagtt tcttcgttta tgtggctggc tacctgacgt
    12301 aatttttctt cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc
    12361 gcggcttcac gaccaatacc atcactggct cccgtcacca ggataatgcg atcattgagt
    12421 aaatcttgtt ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc
    12481 cagtgtactt tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct
    12541 cgggcgataa caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc
    12601 gagacggctt aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg
    12661 gactcgtgga attgttgtct gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc
    12721 tagcgattgc ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc
    12781 gtggcgagtt acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg
    12841 ccgcggcgct gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc
    12901 acaagcaaga agcgaaagca gcaaaagcga aagccaaact gggcgaggtg tcaactgaca
    12961 gtaaaccccg cgtctgggtg ctggacttta aaggcagcat ggacgcccat gaagtgaact
    13021 cgctacgtga agagataacg gcggtacttg cagcattcaa accgcaggat caggttgtgc
    13081 tccgtctgga aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc
    13141 agcgtctgcg tgataaaaac attccattaa ctgttacggt agacaaagtt gctgccagcg
    13201 gtggttacat gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg
    13261 gttccattgg ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata
    13321 ttgatatcga actgcacacc gccgggcagt ataagcgtac gctgaccttg ctgggtgaaa
    13381 ataccgaaga agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta
    13441 aagattttgt gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac
    13501 actggtacgg acaacaggcg gtagagaagg gcctggttga tgaaatcaac accagtgatg
    13561 aagttattct tagcctgatg gaaggccgag aagtggtcaa tgtacgctat atgcagcgta
    13621 aacgactcat tgaccgattc accggcagcg cggcagagag cgccgatcga ttattgctac
    13681 gctggtggca gcgtgggcaa aagccattga tgtaaaagac aaacgcgagg gtaagacctc
    13741 gcgttttgct ttaatcaacc agatgatatt tttctgaaag cacatgggcc aggtgtttga
    13801 acatattaaa caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt
    13861 cgccggtaaa taccagcacg ccattacgct gtgtgacgcc ggtggcttca atccctgcga
    13921 gcgtatcctc atgctcacga atgattttgt tggcctcttt caacagcgtt tcgcggtcga
    13981 tgggttgtgt ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg
    14041 catggcttgc agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga
    14101 ttttgcatgc taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc
    14161 aatctggcaa tagatttgct tgacattcga ccaaaatttc gtcgtgctat agcgcctgta
    14221 ggccaagacc tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt
    14281 gatatccgca gagagcgagt ccatatcagt aactcgttgc cagtggaagg tttatcaacg
    14341 tgcgacgcat tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca
    14401 tcgttgagtc cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg
    14461 tgaaatccag cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga
    14521 gtgccgactc tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc
    14581 tcgtcaaccg tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc
    14641 ctggtaaaga gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct
    14701 atctcgcaac cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga
    14761 ttggtggtga tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga
    14821 tccgccaggc atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg
    14881 cgcgtcgctt tatggaccgc gtggtggggt atatggtttc gccgctgcta tggaaaaaga
    14941 tcgctcgtgg tctgtctgcc ggtcgtgtgc agtcggtggc agtccgcctg gtggtcgagc
    15001 gtgagcgtga aattaaagcg ttcgtgccgg aagagttctg ggaagtcgat gccagcacga
    15061 ccacgccatc tggtgaagcg ttggcgttgc aggtgactca tcagaacgac aaaccgttcc
    15121 gtccggtcaa caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca
    15181 gcgtgctgga acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct
    15241 ctacgctgca acaagctgcc agcacccgtc ttggatttgg cgtgaaaaaa accatgatga
    15301 tggcgcagcg tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc
    15361 tgagtcagga cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat
    15421 atctgccgga aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag
    15481 cgattcgtcc ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg
    15541 cgcagaaact gtaccagtta atctggcgtc agttcgttgc ctgccagatg accccagcga
    15601 aatatgactc cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc
    15661 gtattttgcg ctttgatggc tggacgaaag tgatgcctgc actgcgtaaa ggcgatgaag
    15721 atcgtatctt acctgcagtc gataaaggcg atgctctgac gctcgttgaa ctgacaccag
    15781 cccagcactt taccaagccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg
    15841 aaaaacgtgg tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc
    15901 gtggctatgt gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca
    15961 ccgatcgcct ggaagagaat ttccgcgagt taatgaacta cgacttcacc gcgcagatgg
    16021 aaaacagcct tgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact
    16081 tcttctcgga tttcactcag cagttagata aagctgaaaa agatccggaa gaggggggta
    16141 tgcgtccgaa ccagatggtt ctgaccagca tcgactgccc gacctgtggt cgcaaaatgg
    16201 ggattcgcac agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga
    16261 aagagcgttg caaaacaacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg
    16321 aaggcgaaga cgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccc aaatgcggca
    16381 cggcgatgga cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc
    16441 caacctgcga cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc
    16501 cgatcgttga gtgtgaaaaa tgtggttctg aaatgcacct gaaaatgggg cgtttcggta
    16561 aatatatggc ctgcaccaac gaagagtgta aaaacacgcg taagatttta cgtaacggcg
    16621 aagtggctcc accgaaagaa gatccggtac cattaccgga gctgccgtgc gaaaaatcag
    16681 atgcctattt cgtgctgcgt gacggtgctg ccggtgtgtt cctggcggcc aataccttcc
    16741 cgaaatcgcg tgaaacgcgt gcgccgctgg tggaagagct gtatcgcttc cgcgatcgtc
    16801 tgccggaaaa actgcgttat ctggccgatg cgccgcagca ggatccggaa ggtaataaga
    16861 ctatggttcg ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa
    16921 aggcgactgg ctggtcagca ttttatgttg atggcaaatg ggttgaaggg aaaaaataac
    16981 ctttaattct gtcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca
    17041 tattattttt tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag
    17101 cacctttttt attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt
    17161 ctgatggcgt ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga
    17221 ggtggtcaat cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc
    17281 cgggatcagt aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg
    17341 aagcggcaag cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg
    17401 cgaagtcctg tcgaaagttg atgccataaa atcggttgcc ggtgagcaca cctggccgga
    17461 taaaggctcg ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt
    17521 catcaaaggc tttattgagc gttatccccg cgtttctttg catatgcacc agggttcgcc
    17581 gacacaaatt gctgatgccg tctctaaagg taatgcagat ttcgctatcg ccacagaagc
    17641 gctgcatctg tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt
    17701 agtcactccg gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca
    17761 atatccgttg gtgacatata ccttcggctt tactggacgt tcagaactgg atactgcctt
    17821 taatcgcgca gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa
    17881 aacttacgtc cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt
    17941 cgccgatccg gaccttgtgc gtgttgatgc tcacgatatc ttcagccaca gtacaaccaa
    18001 aattggtttt cgccgtagta cttttttgcg cagttatatg tatgatttca ttcagcgttt
    18061 tgcaccgcat ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ccaatgaaga
    18121 aattgaggtc atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc
    18181 ggcacctttt atgtagcgaa ggtgccgga
//
LOCUS       DBFIFS010000074        18066 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.074, whole genome shotgun sequence.
ACCESSION   DBFIFS010000074 DBFIFS010000000
VERSION     DBFIFS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.074"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(28..891)
                     /gene="potB"
                     /locus_tag="N7A07_002307"
     CDS             complement(28..891)
                     /gene="potB"
                     /locus_tag="N7A07_002307"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU2476964.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(875..2011)
                     /gene="potA"
                     /locus_tag="N7A07_002308"
     CDS             complement(875..2011)
                     /gene="potA"
                     /locus_tag="N7A07_002308"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU2476965.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2261..3487
                     /gene="pepT"
                     /locus_tag="N7A07_002309"
     CDS             2261..3487
                     /gene="pepT"
                     /locus_tag="N7A07_002309"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU2476966.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3536..4657)
                     /gene="roxA"
                     /locus_tag="N7A07_002310"
                     /gene_synonym="ycfD"
     CDS             complement(3536..4657)
                     /gene="roxA"
                     /locus_tag="N7A07_002310"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU2476967.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            4906..6135
                     /locus_tag="N7A07_002311"
     CDS             4906..6135
                     /locus_tag="N7A07_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2476968.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6500..6688
                     /locus_tag="N7A07_002312"
     CDS             6500..6688
                     /locus_tag="N7A07_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2476969.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6746..7300
                     /locus_tag="N7A07_002313"
                     /pseudo
     CDS             6746..7300
                     /locus_tag="N7A07_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7493..7690
                     /locus_tag="N7A07_002314"
     CDS             7493..7690
                     /locus_tag="N7A07_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2476970.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            7683..7895
                     /locus_tag="N7A07_002315"
     CDS             7683..7895
                     /locus_tag="N7A07_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476971.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            7885..8349
                     /locus_tag="N7A07_002316"
     CDS             7885..8349
                     /locus_tag="N7A07_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476972.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            8342..8575
                     /locus_tag="N7A07_002317"
     CDS             8342..8575
                     /locus_tag="N7A07_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476973.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            8581..8880
                     /locus_tag="N7A07_002318"
     CDS             8581..8880
                     /locus_tag="N7A07_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2476974.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            8877..10277
                     /locus_tag="N7A07_002319"
     CDS             8877..10277
                     /locus_tag="N7A07_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU2476975.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            10478..10729
                     /locus_tag="N7A07_002320"
     CDS             10478..10729
                     /locus_tag="N7A07_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476976.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            10726..11136
                     /locus_tag="N7A07_002321"
     CDS             10726..11136
                     /locus_tag="N7A07_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2476977.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            11147..11419
                     /locus_tag="N7A07_002322"
     CDS             11147..11419
                     /locus_tag="N7A07_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU2476978.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            11376..11504
                     /locus_tag="N7A07_002323"
     CDS             11376..11504
                     /locus_tag="N7A07_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU2476979.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            11546..11770
                     /locus_tag="N7A07_002324"
     CDS             11546..11770
                     /locus_tag="N7A07_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476980.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            12022..12228
                     /locus_tag="N7A07_002325"
     CDS             12022..12228
                     /locus_tag="N7A07_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476981.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            12228..13283
                     /locus_tag="N7A07_002326"
     CDS             12228..13283
                     /locus_tag="N7A07_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2476982.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            13296..13631
                     /locus_tag="N7A07_002327"
     CDS             13296..13631
                     /locus_tag="N7A07_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2476983.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            13644..14057
                     /locus_tag="N7A07_002328"
     CDS             13644..14057
                     /locus_tag="N7A07_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HGU2476984.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            14263..14805
                     /locus_tag="N7A07_002329"
     CDS             14263..14805
                     /locus_tag="N7A07_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2476985.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            15061..15342
                     /locus_tag="N7A07_002330"
     CDS             15061..15342
                     /locus_tag="N7A07_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2476986.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(15943..17403)
                     /gene="phoQ"
                     /locus_tag="N7A07_002331"
     CDS             complement(15943..17403)
                     /gene="phoQ"
                     /locus_tag="N7A07_002331"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU2476987.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(17403..18065)
                     /gene="phoP"
                     /locus_tag="N7A07_002332"
     CDS             complement(17403..18065)
                     /gene="phoP"
                     /locus_tag="N7A07_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU2476988.1"
                     /translation="MVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHLPDI
                     AIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKPFHI
                     EEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMETLIR
                     NNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGYLFE
                     LR"
BASE COUNT         4895 a         4262 c         4670 g         4239 t
ORIGIN      
        1 tcctattaac cttttgaata taaagcacta attatctatt tctgttgcct tcttattcag
       61 cagacgagaa gcgcgccagt aaaccagcaa catcaggccc attacgatag tcagcgtaat
      121 gctggtggct gcaccaaacg gccagtcacg aatattgagg aactgtacct tgatgacgtt
      181 accgatcagc aggtttttcg caccgcccat caggtcggac acatagaaca ggcccatcgc
      241 tggcagcatc accagcagac atccggcaat aatccccggc atcgtcagtg gaataatgat
      301 gcggataaaa gtctgtaact tgctggcacc gagatcgcgc gccgcctcaa gcaacggctt
      361 atccagtttt tcgatactgg agtacagcgg catcaccata aacggtagca gaatgtaaac
      421 cagaccgata atcaccgcac tgggcgtgaa catgatacga attggtgtgt cgataacgcc
      481 cagccagagc aaaaactcgt tgagatagcc tttggtgctg aggaaaattt tcagcccgta
      541 gatgcgaatc agtgagttgg tccagaaggg aacaatcagc agaaacagca gcagcggacg
      601 caccttgtgt ggcagcttcg ccagaaacca ggcaaacgga tagcccagca ccaggcaggc
      661 gagggtggcg atcagcgcca tattcagcga gtgcaatagc acttcaaaat agagcggatc
      721 gagcagacgt gtgtagttat ccagcgtaaa gaccattttg acaaaactgg cgtcgtcgcg
      781 ggccaaaaag ctggtgccaa tgatcatcag gttgggcaga aagacaaaca acacaagcca
      841 accgacaata gtgacaatca ctacattctg gaacttactt gtgttcttca tcagccagta
      901 cgacctccca gctttctacc caattaatgg ccattttttg gtcgagagag tggtcaaagt
      961 caggatcgtc ttcattgaag aattcgctga ccatcaccat cttgccattt tccagttcaa
     1021 caaccgactc cagcgtcatg cctttgtagt tacgctcgcg aacgtaacca atcagccctt
     1081 cagcgtggtt gtcgtcgtta atctcttcaa cacgtaagtc ttccgggcgc agcagaacat
     1141 gcagtttttg ccccggttca acggcgaagt taacgtagat attacattcg cggccttcaa
     1201 cgttggcgcg tacgcgctgc tcgtcgagac gttcgatgac agtggcgtta aacatattga
     1261 tttcgccaat gaacccggca acaaacaggt ttttcggctc ttcgtagatt tcacgcggcg
     1321 tgccgtcttg ctcaatgcga ccatcgcgca tcaccacaat cctgtctgac atggtgagtg
     1381 cttcttcctg atcgtgagtc acgaagacga atgtaatgcc aagcttacgc tgtaacgctt
     1441 tcagctcgtt ctgcatttgc ttacgcagtt tgtaatccag cgctgagagc gactcatcca
     1501 gcaacaacag acgaggcttg ttaaccaccg cgcgagcaat ggcgacgcgt tgctgttgac
     1561 caccagagag ctgatgcggt ttacgttgag cgaaggtttc cagctgcacc atccgcaggg
     1621 cttccatcac gcggggcgta atttcagcag cgggggtttt ttgcatgcgc aacccaaagg
     1681 ccacattttc gaacacggtc atgtggggga aaagtgcgta gctttggaaa acagtgttca
     1741 catagcggtt ttccgccgga acgtgggtga tgtcctcgtt atccaacatg atgcgtccgg
     1801 aatcaacagt ttccagacct gcaatcaggc gaagaacggt tgttttaccg cagccagaag
     1861 ggccaagcag cgtgaggaac tcgccattgt tgatagtcag atccagctgg ggaatgacct
     1921 ctttaccatc aaagcatttg cgaattcccg ccaattgcac cagcggtgaa agcgaactcg
     1981 gttgtttatt caatttttta ctctgtccca tgtaaacgca acggatggct taccgatgcg
     2041 gggtttgtgg ttaaccacct tggtgactct taatgagggc ggtaattcta cggcaaaccg
     2101 cttgaatcgc caatctttgt tgtgaattac tggcttagct ttatattcat taaggtaatg
     2161 ctgataaata ttcccgcttg caggggtaaa agtgacctga cgcaatattt gtcttttctt
     2221 gcttcttaat aatgttgtca caaaaagtga gggtgactac atggataaac tacttgagcg
     2281 atttttgaac tacgtgtctc tggataccca atcaaaagca ggggtgagac aggttcccag
     2341 cacggaaggc caatggaagt tattgcatct gctgaaagag cagctcgaag agatggggct
     2401 tatcaatgtg accttaagtg agaagggcac tttgatggcg acgttaccgg ctaacgtccc
     2461 tggcgatatc ccggcgattg gctttatttc tcatgtggat acctcaccgg attgcagcgg
     2521 caaaaatgtg aatccgcaaa ttgttgaaaa ctatcgcggt ggcgatattg cgctgggtat
     2581 cggcgatgaa gttttatcac cggttatgtt cccggtgctg catcagctac tgggtcagac
     2641 gctgattacc accgatggta aaaccttgtt aggtgccgat gacaaagcag gtattgcaga
     2701 aatcatgacc gcgctggcgg tattgcaaca gaaaaacatt ccgcatggtg atattcgcgt
     2761 cgcctttacc ccggatgaag aagtgggcaa aggggcgaaa cattttgatg ttgatgcctt
     2821 cgatgcccgc tgggcttaca ctgttgatgg tggtggcgta ggcgaactgg agtttgaaaa
     2881 cttcaacgcc gcatcggtca atatcaaaat tgtcggtaac aatgttcatc cgggcacggc
     2941 gaaaggagtg atggtaaatg cgctgtcgct ggcggcacgt attcatgcgg aagttccggc
     3001 ggatgaaagc ccggaaatga cagaaggcta tgaaggtttc tatcacctgg cgagcatgaa
     3061 aggcaccgtt gaacgggccg atatgcacta catcatccgt gatttcgacc gtaaacagtt
     3121 tgaagcgcgt aaacgtaaaa tgatggagat cgccaaaaaa gtgggcaaag ggttacatcc
     3181 tgattgctac attgaattgg tgattgaaga cagttactac aatatgcgcg agaaagtggt
     3241 tgagcatccg catattctcg atatcgccca gcaggcgatg cgtgactgcg atattgaacc
     3301 ggaactgaaa ccgatccgcg gcggtaccga cggcgcgcag ttgtcgttta tgggattacc
     3361 gtgcccgaac ctgttcactg gcggttacaa ctatcatggt aagcatgagt ttgtgactct
     3421 ggaaggtatg gaaaaagcgg tgcaggtgat cgtccgtatt gccgagttaa cggcgcaacg
     3481 gaagtaagcg aaaagggatg cggcatgtga tgccgcatcc ggcttaaatc caaacttacc
     3541 cttcgaagaa ccaatacccg ctattgacca gcgccgcgag catcgcgagg aatgacggat
     3601 cttccagcgc atcgccaaaa ttctccgcag tcagcgcaat gttgctggcg agtgcatcca
     3661 gtgccggacg gtgcggggaa tcgatcttct caccattggt atacacgtcg tcgccaatgc
     3721 gcaatacgcg cagaccaccc aggcgcacca gcacatcacc ttgtttcagc gcatcgtaga
     3781 tttcatctgg ctgataaggc ggctccggcg gcgcgatatc cagttcatga cgtgactggg
     3841 atataaactc gccaaaccat tgcttaaagt gttccggctg gttgatcaat tcgagcatca
     3901 tctcacgcag tttatccatc tcttgcggca gaacgtccgc aggatgagcg cgaggtggaa
     3961 catcaggatc gctgtagtag ttgccgccca gttcgcgttg cagcacataa tcggcaaatc
     4021 cgctaattaa ttcccgcgta tttggcgcgc gaaaacccac ggaatagttc atcgcatttt
     4081 ccagcgcgta gccttcatgc gggaatcctg gcggaatata aagaatatcg ccaggctcca
     4141 gctcttcatc gatgatggct tcgaacggat cgacctgtaa cagatccgga tgtgggcagt
     4201 gctgtttcat ttgcagcttc tcgcccactc gccagcgacg acgtccggta ccctgaatga
     4261 taaacacgtc gtactgatcg agatgcgggc cgacgccgcc gccaggtaca gaaaaagaga
     4321 tcatcagatc atcaatacgc cagtccggta gttcacggaa cggtcgcatc agcgcggcgg
     4381 tcggctcatg ccagtggttc actgcttgca ctaacaatga ccagttagtt tcaccgagat
     4441 gatcgtagct ttcgaacgga ccgtggctga cctgccattt tccatcctgg tgactgacca
     4501 gtcgactgtc gacttcgctt tccatcgcca gacccgccaa ctcgtctgga gagatcgggt
     4561 caataaaatt attaaagccg cgttttaaca ccaccgggcg tttctgccag tgacgttcaa
     4621 gaaaatcggg ccagttaaga gtgagttggt attccatgtt aagcatccac aggctggtat
     4681 ctgcaaccga ttataacgga tgcttaacgt aatgcgtgaa gtatggacat attttatcaa
     4741 ttagtgtttt ttgccgtttc ataatgtttc agattgttca ttatattttt gattataaac
     4801 agatttgtga ttttccttgt tccagcttgt ttcacgttgt agccctttgt gcaacgatcg
     4861 gtgtagttaa tggtgtagtt aattgtgatg accggaatca aaattatgag cagagcactt
     4921 aacaaactga gcgatacaca gctgaggaaa atcaacggca cacccgccca aaaaacagcc
     4981 tttcttaatg acggtggaaa cctgagcgtc aggcattcaa ccagtggcct tttaacctgg
     5041 tatttcactt acagggccgg aacgggaagg ggggcaccac cggaacgcat taagctggga
     5101 aattatcctg atctgagcct gaaatcagcc agggaaaaag ccgcccagtg tcgcgcatgg
     5161 ctggcagagg ggaaaaatcc acgtcatgag cttaattaca ccgtacagga agcgttaaag
     5221 ccggtaacgg ttggcgatgc gctcacctac tggcttgagt cgtacgcaaa ggaaaaccgc
     5281 gtggattatg ccgccctgaa aaagcgcctt aataatcacg taatacagca cattggtgct
     5341 atgccgctgg ataaatgcga gctacggcac tggctggcct gttttgacca ggtggcaaag
     5401 cgaacgcctg ttactgccgg attcttgcta cagacgtgca aacaggcgct taagttctgc
     5461 cggaggcggc gctatgcaat cagcaacgtt cttgatgata tgagtgtggc ggacgttggg
     5521 aaaaaaccgg atataagcga gcgtgtctta agcaccaaag aactgggcga attattgcag
     5581 gcactggaca aaaaaatatt ctccccctac tacatcgcgt taatccgcct cctgattgtg
     5641 ttcggatgcc ggacggtaga actgaggtta tcggagatca gcgagtggga ttttaccgaa
     5701 atgctctgga ccgttccgaa ggagcacagc aaaacgaagg tagccatatt ccggcctata
     5761 ccggaagcaa tactgccgtt catcacgcag ctggtggagc agaacaggca cacgggctta
     5821 ttgctggggg aagtgaaaca ggaggccagc gtatcgcagt atggaagatt agcgcacagg
     5881 aggcttaatc accctcactg gtcactgcat gacatccggc gcacctttac aaccatgctg
     5941 aacgatttag gcgttgatcc gcatgtcgtg gagcagctta caggccacca gatgccagga
     6001 atgcagcgag tttataatca ttcccgttat ctggatgcga aacgcaatgc gctggatatg
     6061 tggacggagc ggttagggat actggcggga acacatgaaa acgtaaccac gctaccagta
     6121 gccagaagaa aataattttt ttcgtgtttt ttcagtatgc gcatactgga tatgcgaaca
     6181 gatacaacgt gcaacaatga gcaacaatgt ggaacaacta cgaacaagag gcgaaaaagc
     6241 gtacggattt ataagtagct gatttttaat gtgttactgg ttttttatac actagcgaat
     6301 cactctttaa agagcgagaa aaaaggcatg gaacaatatt ttatgccttt gtattaacat
     6361 gaatttaaaa gattttattc ttactttttc attttctgta aatcatacat cctcagtatt
     6421 tgccattcat cactctagcg ggaacaatac gacacaataa gacacctgat gactctttaa
     6481 gaaacgaaag ggggcaatag tgttaagcac tgatcggttt atacgtgaaa aagaatgcga
     6541 aaaactaacc ggccttagcc gtacgtgtcg ctaccgcctg gaaaaggccg gacaattccc
     6601 atcacgtcgt aaacttggcg gtcgttccgt tggctggtct ttatccgagg ttctggcctg
     6661 gaaggatagc tgcaaggcag ttcattaatc acgctggcgg cacacagccg ccacacatca
     6721 atcatctgaa cacagagcta taaccatgaa gattgaatat acgccagaac gtgggcgggg
     6781 attcgttcgc cctggtgaga ctggaaaacc acaaaattgg ggtttttcag gcataaaaaa
     6841 agcggccccg aaatggagcc gcctttctga acagataacc cgctgcgcct tatgtgtata
     6901 tgatcccaaa cataagcacg gggatgatag ccgctatcag gctggtgggc aatgctatca
     6961 gtctggttca gttcgttgcc atacctgcaa tgggcgcgtt tccctgtact ctttaaggaa
     7021 ttgctcaagg gcaaaagcac atggcgcgaa tctttctgat tcatgctcta tctttctgcg
     7081 ccgtcttttc cgtgccggtg ataatgtttt ggtcaattct ttatcggtca ttgtgttgtc
     7141 ctgcatagca atgcgccgta atacctcaca ccacggcgct gatagttttt atcctttggg
     7201 ttctatgccg cgttgctgta attctttgcg gagaacgcgt ttaatccaag tggctaaaga
     7261 tgtgtcgcca tcggccttag cagcttcttc tagctgcgct ctgaagtctt ccggtaatcg
     7321 catttggtat ggaggcgaac gtttttctaa tggtgttgac atggtaatta ccctttattg
     7381 ctatacctac atggtaatta ccattgtact tgccattaca caaaaatcaa cgccccgcag
     7441 tgctgggaac acatacgggg cgtctaacca acaacgtaaa ctaggagccg ttatggttgc
     7501 cgtaaatcat ataccacacc ttgtacacac acaaacggcc tttgtgtggc gttttctggc
     7561 actgagtgcc ggagaatctc aaatcatcca cgtaaccgcc tggacggaac gcgaagcgcg
     7621 taaccgttgc ccgtccggtt gtgttgctgt atttgccgcc cgtattcgtc aggaggtgcg
     7681 ccatgattaa tctgtcactt actgacctta accgcattca gtttcgtgag aaattcacag
     7741 ggcagctact ggtcaatgtg gataaaggtc gcgtggtgtg taattaccac ttaccagatg
     7801 aagcaattgt cgccacaagg gaatcattac aggaaatcac aaaacgtgcc ggaatgatta
     7861 acacgaatat tcaggggatg ctttatgcac aataaaacca caccggacgc agccgccgaa
     7921 gccatcaaaa cgctgatgca cgcgctgatt gatatttctg ttatcgcgga cagggcacat
     7981 aagcacgcca ccagcgaaac agaatatgcc ggtgctttcg ttcctcactc actggctgtc
     8041 atgcaattta gtgctgatac ggcgctgaat gaggccaaag ctatcctgat tgctgattgt
     8101 gaaaatgggg gggttatgcg tgatgatcgt tttaattccc tgaaacagga attttccggc
     8161 gttcctgatg atgcggctga tgcgctttcg tcaatgccag aacttattag agcggctttt
     8221 ttcttacttt ccacgagaga atataaatca acggggcttg atgtactgaa tatcgccgcc
     8281 gattatgcgg aatatgtggc agaggcgcgt tatagaagaa aatttcctga ggatgtaagc
     8341 catgcgtgat atttaccacc aaacaataga ccgcgcattt cttgcacttt ctcacagtga
     8401 aaacatgatg gaaatattgc gcatatggct tgaaacactt ggcgacaatg aacgcgacaa
     8461 acaaaaatca agaattgcca cggcattaat aacgcttctt gagcctgtaa taatggaact
     8521 gcaagaaata gatctattgc acgacagata taaagaacag cacaccggag aataaaaata
     8581 atgaaactta aatattctgg cttaactgcc agtggcaaca ctcaccctaa atttacgcgc
     8641 ggtgatattt accgcgacca gtacggcggc acggtaatga ttaagggcgt ggaagaacgg
     8701 cgcgtaacct accgccgtga aggttacgaa tatgattgcg tgatgcctgt ttatcagttc
     8761 cggcgtgatt tttctctggt acagaccgcg ccgcataacg tgcccaccag caacgccagg
     8821 gcacgggcaa acatccagaa gctgaaaacc atggttaacg gattcagggg caagaaatga
     8881 aaagcgcacc gaacttaaaa aaacagcctt acgacaagat gaccgaagtc attatttttg
     8941 cgggtagtga tgcctgggca catgcgaaac agtggcagga acaggacggg cgactggctg
     9001 gcgataatgt gcctcccgtt gtgctggctg atgatcaact ggatgaactg gcagaactga
     9061 gaatcatcga cgaggggcgc tattgtgtcc ggctgtacaa ggcaggccac atcaggccat
     9121 caaatattaa tgccattgcg cacaagctgg cggcggcggg tgtaactgat gcgaattatt
     9181 accccgaagg gatgcacagc catatgcggg agaactggcg cgaatacctg gaacgggtgc
     9241 gcgggaaaga gccggcggaa gaaaaaaacc accagcgaaa aaccacgcta ccgatgagcg
     9301 ttggatctac cggatacgac acgcaactgg attacgtggt taaggggatt attccggcgg
     9361 tatcgctatg cagcatatac ggggctagcg ggtcctataa atcattcctt gccggatcgt
     9421 gggcgtgcca tgttgccact ggtcgccagt ggggaggccg cagggttgca catggtgcgg
     9481 ttctctatgt ggttggtgaa ggcggtatag gtgttccgcg tcgtgtaaaa gcctgggagg
     9541 ttgtgcacga tgagcaggtg aaaaatctgt atctggtaaa ccgccccatc tttccggctg
     9601 ccccgcttga tgttgatgaa atggttatcg ctgcccgtca ggtggagcgg gaaacgggta
     9661 aacctgtacg catgattatt ctggatacgc tggcgcgttg ctttggtggg aatgatgaaa
     9721 atgattcccg tgatatgggg gcgtttatcc gtggttgtga cgaactgaaa cgacgcacag
     9781 gggccacggt gctggtggtt caccattccg gcaaggatga gacgaaaggc gcgcgcggtt
     9841 ccagtgcatt tcgtgcttcg ctggatgctg aataccggat acgcagggag gacgcaggaa
     9901 gcgaagcgct ggttatctca tgcaccaaaa tgaaggacgc ggaggaactc aaagaagccg
     9961 catatgactt acgcgtggtg gagcttttta ccgacgctga cggtgaatta atcacgtcgc
    10021 tggtggtggt ggatgatccg cgccctcctg ttgaactgga gcgcatcgag gaggcaggga
    10081 acaagacgga aaaccatacc gcgctatggg ggtgcatccg ttcacgcaca cagaacggcg
    10141 acaagtgcac gatcccgctg ttacgtgatg acatgaaaaa gctggggtat gaaatgaaaa
    10201 acttccggcg ctggctgtac aagctggaaa aagatggggt tattcgtatc gatggggatg
    10261 atgtagcgcc gctataaaag tgaggagcaa aagcgagggg gatagaaaga gggccaaaat
    10321 tagcccgctc tccctcactt ttcgacctgt atacatcctc aaaagtgagg ggtaaaaaaa
    10381 tacttatgaa acacacacat agaaaaaccg aaaatcccaa ctgcgacgaa gtgagacgct
    10441 tgaaaaagtg aggcgaaaaa gtgagaggtt gcgagaaatg acccaaaaac gcagagacag
    10501 aacagagcca aaatataaag cgttagacat gactgagcac accttaaagg tggcaatcag
    10561 aacgatagac cgccacacgc gggaaggata cgcgaaggaa catcccgacc tgataagcgc
    10621 attcatgacc acggcggcgg caaactttgc cacgctgaca gaacgggaga ttgccgaagc
    10681 ggaacaggta acaaccatca acgttaaaac cggagaggtg gaattatgac ggcacagata
    10741 gccgcttacg ggcggctggt ggacgacccg caggtaaaac agaccagcaa gggcacaccg
    10801 atgacgctgg cgcgtatggc ggtatctttg ccatgcagcc aggcacagga cggacaggcg
    10861 acgttatggt tatcggtcat cgcatttggc aaacaggccg gcttcctggc taaacatcaa
    10921 aaaggcgatg ttgccagcgt atccggcacg atgcaggtaa gccagtggac cggacagaac
    10981 ggagaaacgc ggcagggctg gcaggttatc gcagacagcg taatcagtgc ccgcgcggca
    11041 cgtccaggcg ggaacagacg caaaaccaca ggcacacagg gtaatcagcc accagcggga
    11101 ggcgatgacc cttacggtga tgatatttcg ttctgagggg gtgacgatgg tacatgaccg
    11161 catagcggag gaactggagg cgaaaggctt ttaccggagg gcggcggcgc gatggggtga
    11221 agtcatgcag ctggtggaga cagacaagga acggcatcac atcacgatgc gacggctgga
    11281 atgttcaagg aaggcacaga gggcaccgga gccgccggat aatttcggag acctgaaaaa
    11341 ggcagtcgat cgcacttatg ccgaaatggg tatagatggt gctggtgatg aaatatggcg
    11401 caattaccag gacagctaat caaacagccg gagaaatccg gctttttttg caccagttga
    11461 aacggtatgg cgcattaccg ggttttcgtc acggtcaggc atagttacta tctgaaacaa
    11521 acagacacaa cagaggaaaa aaacaatgcc gatgaaattt gatgagatat taaaacagcg
    11581 tgataaatac catgctgaca acatggagac gatgagcatc aatgattacc gcgcattcct
    11641 ggagacgggc gcactgattg aaaaggatca gcatggtttt gtgagatgtg ctctatccgg
    11701 tgaaatgctg gcggtaaatc ctgaacagat agatgcattg atagaatttc tgaaagagat
    11761 cagagactga gccagcacac agcacacata gccggagcaa tccggctttt ttgcgcccaa
    11821 aaaaagcccg ataaggtcag agggttctta tcgggctttt gcatatgagg tttttggatg
    11881 cactggcgtt cgtgatcggg ataatcattt cataatttgc aacataactc aatatcattg
    11941 cataaaatgc aattctgatt ataatcagga ctggataaac atccagttat gattttttta
    12001 gtcgaagagg aatttcttac tatggctgaa gagaaaaaaa gcggtgtttc ggtgtacata
    12061 agccccgaaa tcgtggaggt gctcaagcag cgccacaaaa aaaactatga agctggcgtt
    12121 gcggctggac tggatccgct gatgacgccg gagccgtcga taggttcact tgtacgctct
    12181 tatttacttg cggcgcttgg gatgcataaa aattatgggg gtgaataatg gcaggcaaag
    12241 caacggcact taacactaac cagcttttca tgtacctgaa tcgcggggat attgcggatt
    12301 ttaaattcag ccctctgttt accacgctgt ttttcccgaa cgtggcgaca ttcagcacgc
    12361 aaaacatcat gctggatacc ctggacattg aagaagtcac catgtcggcg ttttgttcgc
    12421 ctatggtggg tagccaggtt cagcgcgata aagggtacga aaccagcaca atcaaacctg
    12481 gctacatgaa gccaaagcac gaaatcgatc caacgaaaac catcatgcgc atggctggag
    12541 aagatccggc acagcttaac gaccctacct atcgccgtat gcgcctgatt actggcaaca
    12601 tgcgccgcca gataaacgcc attaaggcac gcgtggaatg gctggcggtg aatgcggtaa
    12661 cgaccggaaa aaacatcatt gagggcgaag gcatagaacg ctatgaaatc gactggaaaa
    12721 taccggaaaa ctgcatcata gagcaggcca agggtaaaaa atggtccgag caggataaag
    12781 acatgcacga cccaatctat gacatcgagc tttatgctga tcaggctggt tgccccgcaa
    12841 acgtcatgat tatgggcgct gaggtatggc gcacattacg cagctttaaa aaattccgtg
    12901 agctgtacga tctttcccgt ggttcagaat ccgccgccga actggcctgt aaaaacctgg
    12961 gcgaagtggt gagctttaaa ggctatcttg gtgatctggc ccttatcgtc tattccggca
    13021 aatacactga cagcgatggt accgaaaaat atttccttga gcctgatttg ctggtcctgg
    13081 gtaacaccaa caataaaggg ctggtggcct atggtgcgat aatggatcag gaagcggtaa
    13141 gaacgggcgc aacacaaaac atgttttacc cgaaaaactg gattgaggac ggcgatccgg
    13201 cgattgagta cgtgcagaca cacagtgcac cgcagccggt accggcagat attcgcaaat
    13261 ttgttaccgt caaaattggt taacgggggg attctatgga cactccatac attgagttat
    13321 ttgcaggcag tcagcaggta tccacgacgt tggtacattt tgccgctgat gctggcgtta
    13381 ttcaggaatt taccccgctg atgctggcag acaatggcga gtttaagccg tgggatggtc
    13441 aggaatctgg caaggctgtt tatctgacct cgtaccctgt ggacacgtca aagcagaaat
    13501 cagcacagtg ttacaagacg gggatattca atattgccgc cgttaactgg cctgagagcg
    13561 tcgacaccga tgcgaaaaaa tgcgccgcct ttgcgggttc cggcgtatcc gttcagccgc
    13621 tggcccgata agcaggggga acgatggcaa cgaatgaaag catcatgacg ctaccgctgg
    13681 cgagtaaatt taaagtcgaa gcgcgggcaa tggctgacag aggtttatca acctatgagg
    13741 ccgtatatca actaaacaaa ctggaagagc aggacaagcc gtgcgctgat gcgattatgg
    13801 cgcttcatga gcataaagac tatcagccgc tgttgcgtgc aatggcaaac gtgccttgta
    13861 tcgatgttgg tacggctaaa agcatcctta gcatgaccat agagcaggaa cgtccgaagg
    13921 ttgcgccgga acttaccgca gcctttgaaa actttatgga catgcacagc ccgcaagccg
    13981 tatcagctgg catggcatac gatggcagaa accagggcga tgacggcgac atcgatcgca
    14041 tactgaaaac catctgagac aaggccggag aaatccggct tttttacggg tccttcctgg
    14101 aattatggcc cgttacgggg cggcgacctc gcggattttc gctatttatg agccttttca
    14161 gggtggtggt ggtggttttg ttgtttgatc tatctttatg aatgaaaagg gaaagatgca
    14221 agcaatacac caacctgaag cagtaattaa gttggtgtat taatgaaatc tcatctgatg
    14281 aacaaaaaaa acatggcgaa aagctgccgt gtaagtgcga cagcgttcga caagtgggga
    14341 gtaactcccg ttgagcgtaa aggccgtgag gcgttttatg atgttgccag cgtgatagaa
    14401 aatagggtta acaatgcaat tagccagctt ataaacgaca aaggcgagat tgacgatgat
    14461 gaacttttac gagtcaggat cagattactg acagcacagg cagaggcgca ggaacttaaa
    14521 aacgatcgcg atcgcggtga cgtgattgat actgagtttt gcctgtacgc gctttcaaag
    14581 ctggcgagtc agatttcatc aatcatggac agcctcccgc ttactatgca aaggagcttc
    14641 ccacagatta cccccgccat gctggacagg cttaaaaggg aagtggttaa agcctgtaat
    14701 gccagtgcca gagttgccga caacctccca cagatactgg ctgattactt gaaagaaaca
    14761 accggaaacg taccggaaaa gttgctacag aagaaaggcg agtaacagac gcgcaattat
    14821 tgaacaaaaa tgagaaagga tctgataatg gcattttctg gctaaaatgc ctcgtgtcag
    14881 atcctttctt tttttaatat tttcattgtt aaacagtctg ttacagagaa gaagatcgga
    14941 tctgactttt gccggaaaat tctcataaac agataaaaac cgcgaggtcg ccgccccgta
    15001 acgggccata attccaggaa ggacccgacg acaccagact atcagagcga tgggggcaca
    15061 atgacagaag ccgaactact gggattaatc cgccgcgtcg ccggaatcag ccagcagcat
    15121 gacgaacagg ccacacagcc ggacagcgtg accgctgaaa attatgtgcg tgttgtagcg
    15181 gaagtgatgc gccgtgatgg tatagaactt aatggcgttg atatgcgcaa catacggaac
    15241 cgcgttcttg aaatgctggc ctacaatcgc cgcgttgaac tgtatcggga gaaagaaaaa
    15301 ataacgtacc actggaagaa gccggaacgg ctgcggcggt aacttgctga tattcccgat
    15361 aacgcaaaat tgcgctggct acatcctcac atctgaggac tgttaatcag aaggccggaa
    15421 tatattcaca aactacgaaa ttttcgtagt ttaattaatg gtcagattaa tcgctaaccc
    15481 gctgatattt tcggaaacct caatttgagg aagtcggaag cgtcaccctt catattctga
    15541 atggtcataa tgaccacgca gaccatcaag gcaaccacaa cgaacatcgt tacggttgga
    15601 gcttttaccc cactggggga aaggtattac gataatcata acacctaccg aacaggtaaa
    15661 acccaccagc ctgattaaca gttaaccgga aaaaaaatcc ggtagccccg ttatggggat
    15721 ccccatatcg acattaacgc ccctcatgaa ttggtgcgct tccccctgga aagattaccc
    15781 gcctgtatat ttcttgtgtc tatttgttcc acgttgtttc atacagtgca ccgaacggtg
    15841 tagttaatgg tgtagtcaat ttgctgtttt tagcactttt tgcagtgtgg gttgtttaat
    15901 taaatcaatg ggttgccttg ctgtggtgat aaaaggacat atttattcat ctttcggcgt
    15961 agaatgctgg cgaccaaaaa tcacctccat ccgcgcaccg ccaagcatgc tctctccggc
    16021 gacgatttta ccctcatatt gcttggtgat ttcgcgggct accgccagcc ccacaccttg
    16081 cccagggcgt aaagtatcaa cccgttgacc acggtcgaaa atgacctctc gcttgcttaa
    16141 tggaataccg gggccatcat cctcgaccac aatatagaga tgctcgtcgg tttgccttgc
    16201 agaaatttcg acaaactcga ggcaatattt acaggcatta tccagtacat tgcccatcac
    16261 ctcgacaaaa tcgttctgct caccgacaaa gctgatctct ggcgaaatat cgagagagat
    16321 attgacccct ttgcgttgat acactttgtt cagcgccgag gtgagattgt ccagcagtgg
    16381 ggcgaccgga tgcagctcgc ggctgagcaa tgtcccgccg tgcatactgg cacgatgcag
    16441 gtagtagcca atttgctgtg aaatgcggct gatttgctcc agcattaccg gttcagcatc
    16501 actgacgctc atcttttcac tacgtagaga acgcagcgta ctttgcagca ccgccagtgg
    16561 cgttttcaga ctatgggtca ggtcggtgag cgttgtacga tatttgtcgt aacgttcgcg
    16621 ttcacttttt aacaatcggt tcaggtttcg taccagactg gtcagttctc gcgttgtggc
    16681 tggattgagc aattcgcggt tatgttcttc cagttcgcgg acttcttttg ccagggcttc
    16741 gatggggcgt aaactccacc aggcggcgac ccacagcagc gggatcacta acagcagatt
    16801 ggctgagagc acatagataa accagctcca gaccatatag gaacttttta gctccaccgg
    16861 aatggtatcc accaccacaa tggttaactt tggcatccgc gatgttgccg ggtagacgtt
    16921 taccgccacc gagtgggtca tctccgcgtc gtcatcatct tcccgcactt cctgcaactg
    16981 ttgctgtatc gaatgatctc cactcagcaa gaggctggta tcgttaacat ccgcttcaat
    17041 ttcatgaaaa ccattcgatt tcagccagtc aggctggatc atcttcatca gccagggcac
    17101 gtcacgttgc gcccataaaa gctgcccgtt ctcatcataa attagcgtca tggtggggct
    17161 ttgcttgtcg atattttcgg gtaactcgac atgcaactta ttgttttccc acttcgcaag
    17221 ggtatagaac agattgctct cgccacgtaa cagccgaaac gtagttttat cgaaactgac
    17281 gctataaccg atcagcgcga ccattccgta ggcaagcgaa agcaccagta ctaccgctgc
    17341 cgttgccaac agaaaacgta cccgcagcga gagcgggaaa aaaagacgca gtaatttttt
    17401 catcagcgca attcgaacaa atagccctgg ccgcgaacgg tagtaatcac ttcttgcgga
    17461 tattgtgcct gaattttttt gcgcagacgt cccatcagta catcaatggt atggctttcc
    17521 cgcagctccg catccggata gagttgtagc attaacgaat ctttgctgac cactttgcca
    17581 ttattgcgta tcaacgtttc cataatggtg tattcgaacg cggtcagttt gatcacttcg
    17641 tcattaatag ataattcacg gcgagagaga tcaacctgaa acggggggag cgaaatgacc
    17701 tgtgaagcca ggccgctatt acgccgcatt aatgcctgca ttcgcgccat cacctcttca
    17761 atatgaaacg gtttagtcac gtaatcatca gcaccggcac ttaatacttc gactttgtcc
    17821 tgccagcttt cacgggcggt taataccaga atcggcagtg aaacatcatt gctacgccag
    17881 cggcaaatca gtgacagacc gtcctcgtct ggcaatccga gatcgacaat cgcaatatcc
    17941 ggtaaatgtt cattgagata ataatcggct tctttggcat cttctgcatc atcgacctga
    18001 tgaccagcat cctgaatctg aactttaagg tggtgacgta acaacgcatt gtcttcaaca
    18061 accagt
//
LOCUS       DBFIFS010000075        18051 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.075, whole genome shotgun sequence.
ACCESSION   DBFIFS010000075 DBFIFS010000000
VERSION     DBFIFS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.075"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..416
                     /locus_tag="N7A07_002333"
                     /pseudo
     CDS             1..416
                     /locus_tag="N7A07_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            519..1022
                     /locus_tag="N7A07_002334"
     CDS             519..1022
                     /locus_tag="N7A07_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU2476989.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1089..2546)
                     /gene="nuoN"
                     /locus_tag="N7A07_002335"
     CDS             complement(1089..2546)
                     /gene="nuoN"
                     /locus_tag="N7A07_002335"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU2476990.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2553..4082)
                     /gene="nuoM"
                     /locus_tag="N7A07_002336"
     CDS             complement(2553..4082)
                     /gene="nuoM"
                     /locus_tag="N7A07_002336"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU2476991.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4313..6154)
                     /gene="nuoL"
                     /locus_tag="N7A07_002337"
     CDS             complement(4313..6154)
                     /gene="nuoL"
                     /locus_tag="N7A07_002337"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU2476992.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6151..6453)
                     /gene="nuoK"
                     /locus_tag="N7A07_002338"
     CDS             complement(6151..6453)
                     /gene="nuoK"
                     /locus_tag="N7A07_002338"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU2476993.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6450..7004)
                     /gene="nuoJ"
                     /locus_tag="N7A07_002339"
     CDS             complement(6450..7004)
                     /gene="nuoJ"
                     /locus_tag="N7A07_002339"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU2476994.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7016..7558)
                     /gene="nuoI"
                     /locus_tag="N7A07_002340"
     CDS             complement(7016..7558)
                     /gene="nuoI"
                     /locus_tag="N7A07_002340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU2476995.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7573..8550)
                     /gene="nuoH"
                     /locus_tag="N7A07_002341"
     CDS             complement(7573..8550)
                     /gene="nuoH"
                     /locus_tag="N7A07_002341"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU2476996.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8547..11273)
                     /gene="nuoG"
                     /locus_tag="N7A07_002342"
     CDS             complement(8547..11273)
                     /gene="nuoG"
                     /locus_tag="N7A07_002342"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU2476997.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11326..12663)
                     /gene="nuoF"
                     /locus_tag="N7A07_002343"
     CDS             complement(11326..12663)
                     /gene="nuoF"
                     /locus_tag="N7A07_002343"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU2476998.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12660..13160)
                     /gene="nuoE"
                     /locus_tag="N7A07_002344"
     CDS             complement(12660..13160)
                     /gene="nuoE"
                     /locus_tag="N7A07_002344"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU2476999.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13163..14965)
                     /gene="nuoC"
                     /locus_tag="N7A07_002345"
     CDS             complement(13163..14965)
                     /gene="nuoC"
                     /locus_tag="N7A07_002345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU2477000.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15059..15721)
                     /gene="nuoB"
                     /locus_tag="N7A07_002346"
     CDS             complement(15059..15721)
                     /gene="nuoB"
                     /locus_tag="N7A07_002346"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU2477001.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15737..16180)
                     /gene="nuoA"
                     /locus_tag="N7A07_002347"
     CDS             complement(15737..16180)
                     /gene="nuoA"
                     /locus_tag="N7A07_002347"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU2477002.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16811..17749)
                     /gene="lrhA"
                     /locus_tag="N7A07_002348"
     CDS             complement(16811..17749)
                     /gene="lrhA"
                     /locus_tag="N7A07_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU2477003.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4478 a         5048 c         4671 g         3854 t
ORIGIN      
        1 agcggtgcca gagttaccgc gcagggcgtc cccaattggc tattacgact ctgcgcctgg
       61 tagccaggat tacccttacc cgcaatgtcc tggctttatc ccgatcgggg ggactttatt
      121 gcctggtcgg cagaatacag gatatcaacg ccgttcgtca ggtaaaaacg gcattgttat
      181 catcccggga tttatctgtt tattctatga atgccccatg gtttattcac ggtattgatt
      241 tctctgacca ccttaattat tggcaacacg atattctcgc cgtaatgatt actgacaccg
      301 ctttttatcg taataaacaa taccacttgc ccggtgatac agcagacaga ttgaattatc
      361 agaaaatggc tcagatggtg gatggtgtta taactttgtt acacaacagt aaataataaa
      421 aatttctcga ggcgggtcca gaaacagcga catgtacatt ttattttccc tcgtctgttt
      481 taagatgcga aacataaaat tcaatggagg aaaaataaat gggaatgatt ggttattttg
      541 cagaaattga ctcagaaaag attaaccaac tgcttgagtc tactgaaaaa cctttgatgg
      601 ataatataca tgacacgctt tcaggattac gccgactgga tattgataaa cgctgggatt
      661 ttttacactt tgggctgaca ggcacctctg cgttcgatcc agcgaagaat gatccgctga
      721 gtcgtgccgt gcttggtgaa cacagcctcg aagatggtat agatggtttc ctgggattga
      781 catggaatca ggagttagcc gcgacaatcg accgactgga atcactggat cgaagtgagc
      841 tgcgtaaaaa gttttccatt aagcgactca atgaaatgga gatttatccg ggagtgacct
      901 tttcagaaga gttagagggg caactctttg cttcgatcat gcttgatatg gaaaagctga
      961 ttagcgcgta tcgacgtatg ctgcgacaag ggaatcatgc gctgacggtt attgttgggt
     1021 gatggtgtca gggcaccaaa aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa
     1081 ctgatagctt acatcagcgg cattgctaac cgcacaatac taatcagcgg ttgtggccat
     1141 acacccagca ccagtaccaa cagtgcagaa atcagcacca cgataccgcc cgcgctgtac
     1201 tgccagtttg acggtgcatc gcgacccggt tgttccggtg cgtgcaggta caggctcacc
     1261 gccacgcgca ggtagtagta gaggccgatt gccgaaccga caaccacggc acccaccagc
     1321 caccacaggt gtgcctggac accaaccgcc agcacgtaga acttaccgat aaagcccagc
     1381 gtcatcggga taccggccag agacagcatc atcaccgtca tcaccgccgc gaggatcgga
     1441 cgatgccaga acagaccgcg gtaagagaac agggaatcag catccgggcc acgatacggg
     1501 ctggacatca ggctgaccac gccgaacgcg ccgaggctgc tgaacagata accggccagg
     1561 taaaccccta ccgcttccat cgacatctcg ccagtttgca gcgcaatcag cgccaccagc
     1621 agatagccga ggtgagagat agatgagtaa ccgagcagac gtttgatatt ggtctggctc
     1681 agcgccatca ggttaccgaa gatgatggag gcaaaggcga taatcgccag caccacccga
     1741 atcgcttcgc tgtcacccac cggtgcgtag aggaacagac gcatcaccac accgaagata
     1801 gcgattttgc tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg
     1861 tctggcgtcc acaggtggaa cggcaccaga gagagtttga agccgaggcc aacaatcatc
     1921 aggccgaaac ctgccagcaa cagcggctcg tttagcatac cgtcgccgag gtttttaccc
     1981 agcgcgacaa acgacaggtc gccagactgc gcatacacca gcgccatacc gaacagcagg
     2041 aaagaagacg ctgcggcaga aaggatggtg tatttgatac tggcttccag cgaacgtttc
     2101 tggcggaaag cgtaaccgac caggccaaac agcggcaaag agatcagttc aataccgagg
     2161 aacagagacg ccagatggtt tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac
     2221 accaacaggt agaactcatc cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa
     2281 gtacaggtgg cgaggctcgc caacaatacc agcccggtgt aaagcatggc gaaaccatca
     2341 acgcgcatca gcggcgtaac gtccatagcg cccgcctggc caacaaacca gagcgaaacc
     2401 agcgccgcgt taagcccaat aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac
     2461 gcaatggaga gcatcacaac caccaccgtc aagccgacga tcagcaacgg tagcagtgcg
     2521 atcaggtttt gtggagttat tgtcatggcg atttacggcc ttgtagtagt aacggaatta
     2581 acaaaccact gctggatatt gccaatcgcg gagtgcgagg tatccagaat cggctgcgga
     2641 tagaagccca gcagtaccag cagcaccacc agcaacagga tcataaacag ctcacgcagc
     2701 gacatccctg gcagttcctg gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga
     2761 tgtaacatcg ccagcgaata aacagatgca aagaccagcc caaaggtaga gataacggta
     2821 atcaccggga caacctggaa gctgccgaac agaatcataa attcgccgac gaagttaccg
     2881 gtgcctggca tcccgagcgt tgccaccgca aagaacagtg acaaggctgg cagccatttc
     2941 atcttgctcc acagaccgcc catcatgcgc atgtcgcggg tatggatacg ttcataaagc
     3001 tgaccacaaa gaataaacag acccgccgct gacaagccgt gcgcaatcat ctggattacc
     3061 gcgccctggt aggccaactg gctgccggtg tagatagcaa tcagtacgaa gcccatgtgg
     3121 gaaaccgagg tgtaggcgat cagacgtttg atatcggtct gggcgaaggc catccacgca
     3181 ccgtagaaga tgccgataac acccagccac atggcgattg gcgcgaactc tgccgacgcg
     3241 tttgggaaca gcggtaggga gaaacgcagc agaccgtaag cggcggtttt cagcaagatc
     3301 cccgcgaggt ccacggaacc ggcggtcggt gcctgtgagt gcgcatccgg cagccagcca
     3361 tgcagcggaa ccaccggcat tttcaccgca aacgcgatga agaagcccag catcaacaga
     3421 tattccacac cactggacat tggcgtattc agcagctctt catagttgaa ggtccagacg
     3481 ccggtcgcat tgtagtgaac aaaaaccagc gccaggatgg cgatcaacat caccagacca
     3541 ctcgcctggg tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt accatcagag
     3601 gctttatgcc cccacagtgc gatcaggaag tacatcggca ccagcatcat ttcccagaag
     3661 aagaagaaca ggaacatgtc gatggcaagg aacacgccga taacgccgcc caggatccac
     3721 atcaggttga ggtggaagaa gccctgatat ttttcgatct ctttccacga acagagtacc
     3781 gccagcacac cgagcagacc ggtcagcacg accatcagca gcgacagccc gtcaatggcg
     3841 agatggatag agataccaaa acgcgggatc cacggcatgt cgaattcaga ctgccactgc
     3901 ggaattccgg cggattgcgt cagtgaataa ccgccctgca accacagttg cagcgacagc
     3961 gccagcgtca atcccatggt gatcagcgcg atccagcgcg gcaccttgac gccaaagcgt
     4021 tcggtctgcc agcacaggaa gccgccgata aaggggatta gtattaacca gggtaataac
     4081 atagctaagt atgtccctta tatttactcc tgctcaggct atctcactcg ccagcccgaa
     4141 tcccggcagt gctcgcaatc ctcacgtact taagtacgct ccggttgctg tgcgctggcg
     4201 gtatccggtc tgacttcgcc gatgacgcct gtacttcagg aacagttacc taaaatttat
     4261 tcttagttca cagacccggc gcaacgccgg gtatattcac tcagccaaca actcaacgca
     4321 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4381 gcagataacc gttctcgctt aacagcaggc ctttacctgc aaagcgggaa aggacagccg
     4441 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     4501 gcttgacgaa cactttgtcg tacagccagt caaagcccca ggcgttgtac caccaggtgc
     4561 ccagcagacg gcccggcgca ctgttggcga tggaggtcac cagagtacgt ttacccagcc
     4621 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     4681 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     4741 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     4801 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     4861 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     4921 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     4981 ctagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5041 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gcgagaatga
     5101 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgggtcatc aagtgaaaaa
     5161 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5221 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5281 gcagcgtaac cgccccaaca atgcccacca gatgcagaac ttccggcgtc atcaggaaca
     5341 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     5401 gcgcggagac aggcgtcggg cccgccatcg cgtcggcaag ccatgtctgc aacggcaact
     5461 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     5521 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     5581 gttcgttgta aagaatgaac agcgcgaaag cgaggaacac gtcacccaca cgggtcacga
     5641 cgaacgcttt cattgccgct gcgccattct tcggatcggt gtaatagaac ccgatcagca
     5701 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttgtcggcaa
     5761 gcaccagaac caccatgctg gcgataaaca ggttggtgta agcgaagaag cgagagtagc
     5821 cctcttcacc gcgcatatac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     5881 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     5941 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6001 agtcaacgcc aataaaggca gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6061 tcgccgagac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6121 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6181 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6241 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct ggccccagta
     6301 acttccggcg accacaaagg ccagcgcgga agcgttaatc atgatttcca gaccaatcag
     6361 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     6421 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     6481 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     6541 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     6601 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accttgatcg
     6661 ttaacaccga gaatggcgta aacaatcacc accaacatga tggctgacaa aattgccgga
     6721 ccaatccaca cctgcggttt cagccactgg cgttcttgtt cgatttctga accgcccagg
     6781 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     6841 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     6901 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     6961 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7021 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7081 cgtcgattgc catacctgcc atccggtaga agttatactc cgggtatttg cccggaccgg
     7141 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7201 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaaa ccacagaaaa
     7261 tgcagcgtga gaagttgatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7321 ctttttgcag cgagatacag ccgaccgggc aggctaccgc gcagaggtta caggctacgc
     7381 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     7441 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     7501 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     7561 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     7621 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg
     7681 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     7741 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     7801 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     7861 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     7921 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     7981 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8041 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8101 agaagcacgc atcgcaccga gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8161 caccgcgtaa accgccagac cggccatcat caggaagaac aaaatcccga tgttcaggtc
     8221 ggcaaccacc caacccggac tgactggcac aatcgcaaag gccagcagca gcgaggtaaa
     8281 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8341 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccacccc agccaacacg
     8401 gttaggtccg taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     8461 tgccccgcag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     8521 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     8581 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     8641 gtcagccctt cagcgatttc aaccggcaga gtgaccgtgt tgccatcgta actaaaggag
     8701 acgcgtgtac ctgcgtttac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     8761 ggctgcggca tacgacactg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     8821 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     8881 ctggtgaagt aatccagacc attttcgctg gtttcaaaca gacgcacacc cggatcgcca
     8941 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9001 cccggagccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9061 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9121 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9181 cgaatacgga atgtcgcatc tggcgcagca tctttgatac ccgccagttc cgggattttc
     9241 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9301 gtgctgtgca gcgagtgcag ccagcgccaa ctttccagca tgacagtttt gctgtcgtaa
     9361 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
     9421 ccgtcgcttt cagcaaagct ggcagcggaa agcaccagat gggcgttttc catgatcgct
     9481 gtgcgttgat ggtcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
     9541 gtagcagaag cgtgacgatg cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg
     9601 gtttccagtt cggttaacgc ttcttcaagc gatccaccgc ccataatgcc caaccccatg
     9661 ctgttgacgg aacgggcaat catggtgata ccgacgtcag cgccgcgacc tttcagggct
     9721 ttcgcgacgt tagccgcagc ctgaatcacc tctaagctac cggcgttcgt cccggagata
     9781 atcaacggtt tcttcgcacc cgccagtgcc tgcacgatga cgtcgatttt gctttgcagc
     9841 tcaggttcaa taccgtcaac cgctggcgca gagttatcca gcgcatgggc gatggcaaaa
     9901 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
     9961 cgggtgtcat caacgttggt aacaaacagc ggatgcttag cacgttgacc gatattgagg
    10021 attgccgcaa tctgccagtc agccactttc tgtgctgccg ccatttcgcg cgctttacct
    10081 ttcaccgcct ggcgcactgc cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10141 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10201 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10261 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10321 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10381 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    10441 cgatctttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    10501 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccataacg ctcgccgggg
    10561 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    10621 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    10681 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    10741 ttgtcatgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    10801 acgcagcggt agcaggcgat gcagcggttc atttcgtgag agatgaacgg ccccaaatcc
    10861 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    10921 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    10981 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11041 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11101 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11161 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11221 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11281 cttccagttt tctcagtcag agactgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11341 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgatccc
    11401 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca cagcaccagg
    11461 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    11521 gatatcgccc ggctgacctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    11581 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    11641 gttacgcacc agcgacacca tgttgatttc atggtcaacc gccatcgcca gcgccgtacc
    11701 cagacggctg cccgctttac cgatactttc gaattccatc ggcaggtcaa ggtgcgcttc
    11761 ggtcaggaag tcggtgcccg cgccgcctgg ctgccaggct ttgaatttca gaccgtcacg
    11821 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    11881 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    11941 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12001 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12061 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12121 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12181 cataatgttt ttgccaagca atcccgcttc agtcgcttcg gcaatggcgc ggcgcagatt
    12241 tactgccgct tcaatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12301 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12361 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    12421 ggattcgtct ttcggcatca ggctccactt caagccagtc gaaaagcccg cgccgccgcg
    12481 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    12541 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    12601 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    12661 catttatacc gctccagcag ttcagggatc gcttccgggg tcagattcgc gtgagtgtcc
    12721 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    12781 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    12841 gcctgaatac cctgataccc attgatatga cagaccacgc tgtcgcaata acggatcaca
    12901 tggcggccaa ccggctggcg gaagatctgg ctgtagaacg ttgccacgcc ttcgacgtcg
    12961 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13021 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13081 tgcatctcgt gctcgatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13141 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13201 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13261 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13321 tcaggtagta actgttgatc cctttggtcg cctcaatcat ctggaaggat tcattggcag
    13381 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    13441 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    13501 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttaa
    13561 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    13621 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    13681 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    13741 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    13801 tcggcatcca gtcgaggaac tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg
    13861 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    13921 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    13981 cctgaataaa ggtcgagata tacagcaggt ggctgttgat gcggaacagt tcggagagca
    14041 ttacgcgaat gacgttaacg cgagccggca cggtgatccc ggccagtttc tctaccgcca
    14101 gcacgtaagg catttcgtta acgcagccgc cgaggtattc gatacggtcg gtatacggaa
    14161 tgtagctgtg ccaggactgg cgttcgccca ttttctccgc accacggtgg tggtagccga
    14221 tgtctggtac gcagtcgaca atctcttcgc cgtcgagctg caaaacgata cggaaagcac
    14281 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14341 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    14401 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    14461 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    14521 atgtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    14581 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    14641 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    14701 cgcggtgcgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    14761 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    14821 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    14881 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    14941 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagcaag
    15001 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15061 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15121 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15181 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15241 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15301 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15361 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    15421 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    15481 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    15541 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    15601 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    15661 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    15721 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    15781 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    15841 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    15901 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    15961 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16021 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16081 cgcctaccag catcaggcaa cataggccaa tggcaacgat aagaaagata gcgaatgccc
    16141 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16201 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16261 aaaaaacctt acaatcactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16321 tcctttctat aaccttttgt caactttaac aaaagtttct tcacattagt ttacataata
    16381 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    16441 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    16501 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    16561 tgtcattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    16621 ggtgattttt ttcaggcgat tatttgtgcg tacggggcgt gaatctctgg tgattgaaaa
    16681 atgaacagtt ttgtacgttc tgcacgatgt gaagaaggtt ctttactaaa aaaaagccgc
    16741 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    16801 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    16861 atattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    16921 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    16981 gtaagccatc tactccactc agcacacgca agtccggact catcatctcg accggccttg
    17041 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17101 aagccagtcg ccagggaata tctgctttat taagcgtcgc cagcaccata tcgcgaaacg
    17161 gacttggatc atccagcaat acaagaggga tcggctcacc tttttgcaat acgtattccg
    17221 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17281 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17341 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    17401 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    17461 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    17521 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    17581 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    17641 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    17701 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    17761 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    17821 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    17881 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    17941 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18001 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc g
//
LOCUS       DBFIFS010000076        18014 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.076, whole genome shotgun sequence.
ACCESSION   DBFIFS010000076 DBFIFS010000000
VERSION     DBFIFS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.076"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            83..751
                     /locus_tag="N7A07_002349"
     CDS             83..751
                     /locus_tag="N7A07_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU2477004.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVYAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            795..5561
                     /gene="ehaG"
                     /locus_tag="N7A07_002350"
     CDS             795..5561
                     /gene="ehaG"
                     /locus_tag="N7A07_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU2477005.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5929..7584
                     /gene="lldP"
                     /locus_tag="N7A07_002351"
     CDS             5929..7584
                     /gene="lldP"
                     /locus_tag="N7A07_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU2477006.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7584..8360
                     /gene="lldR"
                     /locus_tag="N7A07_002352"
     CDS             7584..8360
                     /gene="lldR"
                     /locus_tag="N7A07_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU2477007.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8357..9547
                     /gene="lldD"
                     /locus_tag="N7A07_002353"
     CDS             8357..9547
                     /gene="lldD"
                     /locus_tag="N7A07_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU2477008.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLLMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9733..10206
                     /gene="trmL"
                     /locus_tag="N7A07_002354"
     CDS             9733..10206
                     /gene="trmL"
                     /locus_tag="N7A07_002354"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU2477009.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10259..11080)
                     /gene="cysE"
                     /locus_tag="N7A07_002355"
     CDS             complement(10259..11080)
                     /gene="cysE"
                     /locus_tag="N7A07_002355"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU2477010.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11160..12179)
                     /gene="gpsA"
                     /locus_tag="N7A07_002356"
     CDS             complement(11160..12179)
                     /gene="gpsA"
                     /locus_tag="N7A07_002356"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU2477011.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12179..12646)
                     /gene="secB"
                     /locus_tag="N7A07_002357"
     CDS             complement(12179..12646)
                     /gene="secB"
                     /locus_tag="N7A07_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU2477012.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12709..12960)
                     /gene="grxC"
                     /locus_tag="N7A07_002358"
     CDS             complement(12709..12960)
                     /gene="grxC"
                     /locus_tag="N7A07_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU2477013.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13102..13533)
                     /gene="yibN"
                     /locus_tag="N7A07_002359"
     CDS             complement(13102..13533)
                     /gene="yibN"
                     /locus_tag="N7A07_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU2477014.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13778..15322
                     /gene="gpmM"
                     /locus_tag="N7A07_002360"
                     /gene_synonym="pgmI"
     CDS             13778..15322
                     /gene="gpmM"
                     /locus_tag="N7A07_002360"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU2477015.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15356..16615
                     /gene="envC"
                     /locus_tag="N7A07_002361"
     CDS             15356..16615
                     /gene="envC"
                     /locus_tag="N7A07_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU2477016.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16619..17578
                     /gene="yibQ"
                     /locus_tag="N7A07_002362"
     CDS             16619..17578
                     /gene="yibQ"
                     /locus_tag="N7A07_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU2477017.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17565..18014)
                     /gene="waaH"
                     /locus_tag="N7A07_002363"
                     /pseudo
     CDS             complement(17565..18014)
                     /gene="waaH"
                     /locus_tag="N7A07_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         4510 a         4648 c         4710 g         4146 t
ORIGIN      
        1 tcaccaataa tacaatggac aactctaccg gcattgatcg ctgggcggag gaaataagga
       61 tttttacatg aactagaaga agattttttt cagtgctgtc actgtttcag ttctctgcgc
      121 attaacggga tgtgactaca ttgaagaagg caagccagaa agtagtttat tgaaacaaca
      181 agaagaacat aacaataaaa ttgatctact tgaaaaacaa caagcacagc taaagagtca
      241 actggaaact attcaaaaac aacaaactgg cataataagc agtacaaaga ctttaactca
      301 cgtaattaaa tccgttaaag atcaacaaaa cacttttatt tttaccgagt ttaacccagc
      361 aaaaacgaag tatttcatcc tgaacaatgg ttcggtggct ttagcgggtc gagttttatc
      421 cattgacgcc acagagaatg gcagtgttat tcatatttca ctggtcaact tattaagtac
      481 acctatctca aatattggtt ttaatgcgac atggggtggt gaaaaacctg tctatgccaa
      541 agagtttgcc cggtggcaac aattactttt caacacatca atgaaatcca cattgaaatt
      601 attaccaggt caatggcaag acattaattt gaccctgaag ggtgtatcgc ccaataacct
      661 gggatatctg aagttagcca tcaacatgga aaatattcag ttcgacaatc ttccctctgc
      721 tgaaaatcgg cagaaaagaa gcaaaaaata aatgcagtta gtacgcttat aacaaggaat
      781 agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt
      841 atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt
      901 taatttctgc gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg
      961 ggaatgacaa cggtcagggt gttgattacg gtagtggatc agctggcgac ggctgggttg
     1021 ctataggcaa aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg
     1081 tgggttatga cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg
     1141 cgattggtgg tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta
     1201 tagcactggg tgcctcttcg tattcattgg gccaatactc aatggccctc ggccgttatt
     1261 caaaagcatt gggtaaattg tctattgcta tgggggactc ttccaaagcg gaaggagcaa
     1321 acgccattgc cctgggaaat gccactaaag ctactgagat tatgagtatt gctcttggcg
     1381 acaccgccaa tgcgtcaaaa gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg
     1441 aagaaaacgc tattgcgata ggtgctgaga ccgaagccgc tgaaaatgca actgctattg
     1501 gcaataatgc gaaggcaaaa gggactaata gcatggcaat ggggttcgga agccttgccg
     1561 ataaagtcaa tactatcgca ttaggaaatg gcagccaggc tctggcagat aatgcaatcg
     1621 ccataggcca gggcaacaaa gctgatggcg tggatgccat cgctctgggt aatggtagcc
     1681 agtcgagagg cttaaacacc attgccttag gcacagccag taatgcaact ggtgataaga
     1741 gtcttgcgct tggtagtaat agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag
     1801 attccattgc ggatttagac aataccgtct ctgtcggcaa tagttcatta aaacgcaaga
     1861 tcgttaatgt gaaaaatggc gcgatcaagt ctgacagtta cgatgccatt aatggttcac
     1921 agctttatgc cattagcgac tcggtagcaa aaaggcttgg aggaggggct gcagtagatg
     1981 ttgatgacgg tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg
     2041 taggggctgc gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca
     2101 aatacagcgc tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg
     2161 atggcacgat ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca
     2221 atgacgatgt cgaagccaac accgccaata tcgctactaa taccagcaac attgccacga
     2281 atacggcaaa tattgccacc aataccacca atatcaccaa cctgacggat tccgttggtg
     2341 accttcaggc tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg
     2401 gccaggatac caccagcaaa atcaccaacg ttaaagatgc cgacctgacg gctgacagca
     2461 ctgatgctgt taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca
     2521 ccaatatcgc caataacact tccaatattg ccactaacac caccaacatc tctaacctga
     2581 ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat
     2641 tcacggcagc tcatggcacc gagaccacca gcaaaatcac caacgttaaa gatggcgacc
     2701 tgacgactgg cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg
     2761 tggcgacgaa taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga
     2821 cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg
     2881 cagctcatgg caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg
     2941 caaccagttc cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca
     3001 cctacttcgg cggcaatgct tctgtgaata ctgacggtgt gtttaccggt ccaacctaca
     3061 aaatcggtga aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat
     3121 ttagcacgtc tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca
     3181 aacacggtac taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag
     3241 actccagttc tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg
     3301 atgcgctggg gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca
     3361 ccattgctaa tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc
     3421 ttgacgacgc tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc
     3481 acggaaaaga taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg
     3541 ccagcagcga cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg
     3601 cgctgggtgg tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca
     3661 ttgcgaacgc cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc
     3721 tgctgtggga tgagactgcc aatggcggtg ctggagccta caatgccagc catgacggta
     3781 aagccagcat catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag
     3841 tgaacggttc tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca
     3901 atcagctcgc tggtaacacc gacgcaacct atatccaaga aaacggtgcg ggtattaact
     3961 atgtgcgtac taacgacgac ggcttagcgt tcaacgacgc cagcgcacag ggtgttggcg
     4021 ctacagctat aggttataac tctgtcgcca aaggcgatag cagcgtagct attggtcagg
     4081 gcagctacag cgacgttgat acgggtatcg ccctgggtag cagctctgtt tccagccgag
     4141 tgattgccaa aggctcccgt gacaccagca taacggaaaa tggcgttgtt attggttacg
     4201 acaccacgga tggcgaactg ctcggtgcat tgtctatcgg tgatgacggt aaatatcgtc
     4261 aaatcatcaa cgtagccgat ggttccgaag cccatgacgc cgttacggtt cgtcaattgc
     4321 agaatgcgat tggtgcggtc gcaaccacgc cgactaaata cttccacgct aattcaacgg
     4381 aagaagattc actggcagtg ggaactgact cgctggcaat gggtgcgaaa accatcgtga
     4441 atggcgataa aggtattggt atcggttatg gtgcctacgt ggacgcgaat gcacttaacg
     4501 gcattgccat tggtagcaat gcgcaagtca ttcatgtcaa cagtattgcg ataggtaatg
     4561 gttctacgac cactcgtggc gctcaaacca attataccgc ctacaacatg gacgcaccgc
     4621 agaactctgt cggtgaattc tcagtcggta gtgcggatgg tcaacgtcag atcactaacg
     4681 tcgcagcagg ttcggctgat accgatgcgg tcaacgtggg tcagttgaaa gtaacggatg
     4741 cgcaggtttc ccagaatacc cagagcatta ctaacctgga taatcgggta acgaatcttg
     4801 attcacgcgt caccaatatc gaaaacggta ttggcgatat cgtcaccacc ggtagcacca
     4861 agtacttcaa gaccaatacc gatggtgtag atgccagcgc gcagggtaaa gatagcgtcg
     4921 cgattggttc cggctccatt gctgccgctg acaacagcgt cgctctgggt acagggtctg
     4981 tggcaaccga agaaaatacg atctctgtag gttcctctac taaccaacgt cgtatcacca
     5041 acgtagctgc aggtaaaaat gctaccgatg ctgttaacgt ggcacagttg aagtcttccg
     5101 aagctggcgg tgtacgttac gacaccaaag ctgatggttc tatcgactat agcaatatca
     5161 ccctcggtgg cggcaacggc ggtacgactc gtatcagcaa cgtctccgct ggcgtcaaca
     5221 acaacgacgt ggtgaattac gcgcagttga agcaaagcgt gcaggaaacg aagcaataca
     5281 ccgatcagcg aatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg
     5341 gtggtatcgc ttctgcaatg gcaatgaccg gtctgccgca ggcttacact ccaggtgcca
     5401 gcatggcctc tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat
     5461 cgatggtgag cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg
     5521 gtgaatactc cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt
     5581 gactaagggc aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct
     5641 ccgggtcaac aatctaaggg caattctctg atgaggattg cccttttctt taccagacat
     5701 ctccccccac aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt
     5761 ccccaattaa aactcacatc aatattgcca atacataaca tttagttaac cattcattgt
     5821 cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat
     5881 gagcaacaga ctcattacac gatgtgcgtg gactccagga gacctgcaat gaatctctgg
     5941 caacaaaact acgatcccgc cgggaatatc tggctttcca gtttgatagc atcgcttccc
     6001 atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca
     6061 tggacggtag caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac
     6121 gcgctggcct cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt
     6181 attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc
     6241 tcgtctattc tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt
     6301 ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca
     6361 ttgctggtcg gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac
     6421 accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca
     6481 ggcatcgaca gctttgagat tggccagatg gtggggcggc agctaccgtt tatgaccatt
     6541 atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa ggagacgtgg
     6601 cctgcggtcg tggttgcggg cggctcgttt gccatcgctc agtaccttag ctctaacttc
     6661 attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg
     6721 ttcctcaaac gctggcagcc agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag
     6781 gttgatatga cgctagccca caccggttac actgcgggtc aggtgttacg tgcctggaca
     6841 ccgttcctgt tcctgacagc taccgtaaca ctgtggagta tcccgccgtt taaagccctg
     6901 ttcgcatcgg gtggcgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat
     6961 aaactggttg cccgtatgcc gccagtggtc agcgaggcta cagcctatgc cgccgtgttt
     7021 aagtttgact ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt
     7081 gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa
     7141 ctggctctac ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat
     7201 tccggactgt catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc
     7261 ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac
     7321 gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg
     7381 ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc
     7441 gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact
     7501 gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat
     7561 gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg
     7621 ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg
     7681 ctgagcgcca actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg
     7741 caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct
     7801 ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg
     7861 atgatccgga ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg
     7921 catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg
     7981 aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg
     8041 cgattgccga agcctcacat aacatcgtgc tgctgcaaac catacgcggt ttcttcgatg
     8101 tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt
     8161 cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg
     8221 gggcgcgtaa agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg
     8281 atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt
     8341 cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg
     8401 cattctgccg ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct
     8461 gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat
     8521 gtctgactta agcctggaaa cgacgctgtt taatgagaaa ttgttgatgc cggtagcact
     8581 ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc
     8641 ggcggacgcg catggcattc cgtttactct ctcaacggtt tccgtttgcc cgattgaaga
     8701 agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg
     8761 ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac
     8821 cgtggatatg ccgacaccgg gagcgcgtta tcgtgatgcg cattctggga tgagcggccc
     8881 aaatgcggca atgcgccgct acttgcaagc ggtgacgcat ccgcaatggg cgtgggatgt
     8941 gggcctgaac ggtcgtccgc atgatttagg taatatttcg gcttacctcg gcaaaccgac
     9001 cggactggaa gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa
     9061 agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga
     9121 tccggaagat gcgcgcgatg cagtacgttt tggtgctgat gggattgtag tttctaacca
     9181 cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga
     9241 tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt
     9301 cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc
     9361 gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg agaaagagat
     9421 gaaagtggca atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct
     9481 ggtgcagggg ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc
     9541 ggcatagtcg tttgagcccc ctcaccctaa ccctctccct cagggagtgg ggaccgttcg
     9601 gcgctgtatg tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc
     9661 tccggggtga ggggaaaagg ccgcactgct caccaatttt ctgctatcct gcccccgcac
     9721 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
     9781 aatatcatcc gtctttgcgc taataccggc tttcgtctgc atatcatcga accgatggga
     9841 tttgcctggg acgataagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
     9901 gttacgcgtc atcatgactt tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
     9961 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa actatcagga tggcgattat
    10021 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    10081 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaacct gtccaatgcg
    10141 gtgtcagttg tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    10201 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    10261 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    10321 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
    10381 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    10441 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    10501 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    10561 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    10621 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    10681 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    10741 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    10801 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    10861 cagcggcgta ggcttcttcc accacttcac ggatagcaat agcaggcata attggcgatg
    10921 acagcttgtt cgccagcatg tagctcagtg cactaccaag gttttcgtgc ttgagtagcg
    10981 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    11041 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    11101 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    11161 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc
    11221 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttctacgccg
    11281 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    11341 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    11401 ttacgcgact ggttgtcggt acaagtaagc acaagatcgc ccagccccgc catgcccata
    11461 aaggtggcag gatcggcacc cagcgccgcg ccaagacgcg acatttcagc cagcccacgg
    11521 gtgatcagtg ccgtacgcgc attcgcacca aaaccgatac cgtcggacat ccccgcgcca
    11581 atggcaatga cgtttttcac cgcaccgcca agctgcacgc caatgaaatc cggattgctg
    11641 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    11701 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    11761 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    11821 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    11881 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    11941 agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    12001 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    12061 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtgatggca
    12121 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    12181 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    12241 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    12301 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    12361 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    12421 acaggaacgc ggtttcttcg cccagggagg ccgttacggt aacacgcagt accacttcgt
    12481 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    12541 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    12601 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    12661 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    12721 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    12781 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    12841 cttggcggca ttgccatcga tcggcaactc ctgaaaactc acgcccttgc tgctcagcag
    12901 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    12961 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    13021 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    13081 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaaa ggcaagtttt cgccagccca
    13141 gccagcgacg ccttctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagtgcgtt
    13201 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt tgtctttgtg
    13261 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    13321 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    13381 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    13441 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    13501 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    13561 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    13621 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    13681 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    13741 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    13801 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    13861 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    13921 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    13981 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    14041 caaagatcgc gctttctttg ctaatccagt gctgactggc gcggtagata aagcgaaaaa
    14101 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    14161 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    14221 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    14281 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    14341 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    14401 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    14461 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    14521 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    14581 cgaaatcact cgtgcattcg taaacgcaga tttcgacggc ttcgcgcgta agaaagtggt
    14641 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    14701 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    14761 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    14821 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    14881 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    14941 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    15001 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    15061 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    15121 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    15181 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    15241 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    15301 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    15361 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    15421 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    15481 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    15541 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    15601 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    15661 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    15721 tgccgcattc cgtcagggcg agcataccgg tattcagctg attctcagcg gtgaagaaag
    15781 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    15841 cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga
    15901 gaaacagagc gagcaacaaa cgctgttata tgagcagcgc gcccaacagg cgaaactgac
    15961 tcaggcgctg aacgagcgta aaaagacgct ggcagggctg gagtcttcca tccagcaagg
    16021 tcagcaacag ttgagcgagc tgcgcgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    16081 tgcggaagcc gcggcgaaag cgcgtgcaga acgagaagca cgtgaggccc aggcggttcg
    16141 cgaccgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    16201 atcgctgatg tcccgaactg gtggcctggg ggcgccgcgt ggtcaggcat tctggccggt
    16261 tcgcgggccg acgctgcatc gctatggtga acagctacag ggcgaactac gctggaaagg
    16321 aatggttatc ggtgcctctg aaggtactga agttaaagcg attgccgatg gtcgggtgat
    16381 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    16441 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    16501 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    16561 cgaaattcgc cgccaaggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    16621 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    16681 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    16741 ccaggtgctg gcgatgccct ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    16801 cagagagatg gcgaccaaag cgcataacag cgggcacgaa gtgttgattc atctcccgat
    16861 ggcaccactg agtaaacagc cgctggagaa aaatacgctg cgcccggaga tgagcagcga
    16921 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    16981 caaccacatg ggtagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    17041 actggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    17101 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    17161 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    17221 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    17281 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    17341 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    17401 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    17461 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    17521 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    17581 gcgctacgcg taatgcgatg gtgcgaccgc gtttttttcg cgccattgcc ataagcggaa
    17641 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    17701 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    17761 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    17821 ataaatcata attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    17881 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    17941 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcgtt
    18001 aaacatgaat tctg
//
LOCUS       DBFIFS010000077        17809 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.077, whole genome shotgun sequence.
ACCESSION   DBFIFS010000077 DBFIFS010000000
VERSION     DBFIFS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.077"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1922
                     /locus_tag="N7A07_002364"
     CDS             <1..1922
                     /locus_tag="N7A07_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HGU2477018.1"
                     /translation="NHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKAT
                     QFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSV
                     ADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSP
                     AVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADVNKKL
                     MSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVADELIN
                     NEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTEDIER
                     IAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQ
                     HDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDE
                     EMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVVSMKN
                     STRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTP
                     ASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVKTGAT
                     RKIRNEARHRLEEKQ"
     gene            1919..2368
                     /locus_tag="N7A07_002365"
     CDS             1919..2368
                     /locus_tag="N7A07_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477019.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(2453..3364)
                     /gene="rdgC"
                     /locus_tag="N7A07_002366"
     CDS             complement(2453..3364)
                     /gene="rdgC"
                     /locus_tag="N7A07_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU2477020.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3489..4397
                     /gene="mak"
                     /locus_tag="N7A07_002367"
     CDS             3489..4397
                     /gene="mak"
                     /locus_tag="N7A07_002367"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU2477021.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4540..5724)
                     /gene="araJ"
                     /locus_tag="N7A07_002368"
     CDS             complement(4540..5724)
                     /gene="araJ"
                     /locus_tag="N7A07_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU2477022.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(5850..8993)
                     /gene="sbcC"
                     /locus_tag="N7A07_002369"
     CDS             complement(5850..8993)
                     /gene="sbcC"
                     /locus_tag="N7A07_002369"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU2477023.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(8990..10192)
                     /gene="sbcD"
                     /locus_tag="N7A07_002370"
     CDS             complement(8990..10192)
                     /gene="sbcD"
                     /locus_tag="N7A07_002370"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU2477024.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            10382..11071
                     /gene="phoB"
                     /locus_tag="N7A07_002371"
     CDS             10382..11071
                     /gene="phoB"
                     /locus_tag="N7A07_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU2477025.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11129..12424
                     /gene="phoR"
                     /locus_tag="N7A07_002372"
     CDS             11129..12424
                     /gene="phoR"
                     /locus_tag="N7A07_002372"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU2477026.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            12831..14150
                     /gene="brnQ"
                     /locus_tag="N7A07_002373"
     CDS             12831..14150
                     /gene="brnQ"
                     /locus_tag="N7A07_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU2477027.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14226..15599
                     /gene="proY"
                     /locus_tag="N7A07_002374"
     CDS             14226..15599
                     /gene="proY"
                     /locus_tag="N7A07_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU2477028.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            15755..17572
                     /gene="malZ"
                     /locus_tag="N7A07_002375"
     CDS             15755..17572
                     /gene="malZ"
                     /locus_tag="N7A07_002375"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU2477029.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         4108 a         4411 c         4767 g         4523 t
ORIGIN      
        1 ttaatcatga tttcgcgtat gtcaaaggac agatgacagc gaaaggttta gtttatgctg
       61 attacaatga tcataacttt gaagtgatga agggaattag tgcgcgaggc attattgttt
      121 ccgataaagc aacgcaattt gaagtcatta aagctgaatt ttatatcaat gaagatggaa
      181 gtggtgaagg atataactgg gatgagaata ttcaaaaagc ctattcactg gtgactgccg
      241 atctttatga tcacactgaa atcgaaaccg ataacatttc caatgcgtat cctgactatg
      301 atagcgtcgc agacaacatc gttcagggtt taccgctttt tcgagataaa gccgctcctg
      361 aaattaatga gaaattaaaa tggattgaaa cggggaaact cgataatttc ccagcgaata
      421 aaataaagca tcaagatcca ctggtggcac gttttctgac tcatacagag agtttatcac
      481 ccgccgttat gctgcaactg ttacagcacc ccgatgacca gacgcgtgaa tcgatggcgc
      541 agtcctggcc cgctcaacag atgcattggc ttaccgatga attaattaaa gatgaggctg
      601 ttgccagagg gctggttaaa aacagtaata tttccgccga tgtgaataaa aaattaatgt
      661 ctgtacccgt cgagagcgta cagctggagc aggccagaca ggataatctt tctccagata
      721 ttgttgcgtc gttaagtcat agcccctttc tcagtgtacg caagacatta ctcagccatt
      781 acgattacgc ctggctggtt ccgactgcag tggccgatga gttgataaat aacgaagacc
      841 cggagttacg tgaaagaatt accggagcag atttaaccgc acaacaagca gtgatgttga
      901 gtaaagataa atcactgaaa gttcgtgaag cattagcccg gacgcttact gaattaaaaa
      961 ttacccaact gtcagcaacc ctgcgtacag aggatattga acgtatcgca gaacaaatgt
     1021 atctggataa taaagaaaat aaaaatatcg taaaggtgtt attgatagcg ttgccggaga
     1081 tgcgtcaact gtctctggca aaagaagatg tgcacaattt gcgcgaaggt gcgcgttatc
     1141 tgacatcgaa ggacgtgatt agttatctat tgactcagca tgatgttccc accgtctggg
     1201 atgagctggc acgcgataaa ttattgccgc tggaatataa gaaacagtta tggcaacgca
     1261 cattaaattt aatgatgagc aaacggcaag aagatcaaga gcaagcctac gaagtacagc
     1321 tggcgctgat tgataatggt gttgttgacg aagagatgtt aaataatgcc attgatttac
     1381 tggttgattt acccgctgaa taccgctatc gaatgcgtaa ccaattattt gataataaag
     1441 aattaccttc cggaattatc aacaaactcg atcaacagta tcgcttcaac tctgactggg
     1501 cgctggctgt tgtcagtatg aaaaatagca cccgtagaca aagtgaaaga ggattacacc
     1561 gctggaacca tgaggatagc gatatttttg cggaactggc gacgataaaa gataaatcag
     1621 atgatgaatg gtggcgtgca ttgctgcaaa gtcgcaatga tcatttgcgc caaacggcat
     1681 tacgcaacgc acataccccg gcgtcgttgt taacgacatt gactgagtct caggatcggt
     1741 cgctggctat taataatcca cagctggctg ccgatgtgaa aacggtgtgg ctaaaagagg
     1801 atccatcatt acttttattt gtcgataaac ccgatctttc gcagttacgg gatttagtga
     1861 aaaccggggc aacgcggaaa attcgcaatg aagcgcgtca ccggcttgag gaaaaacaat
     1921 gacacaacgc ccctggagca agttacagcg ggagatctat gacttactga cgccaacgat
     1981 caacctgcaa attcactgta cccgttatcc catgcgcagc caaaatggtg gaagcaccga
     2041 tttaccgcgt tattggatca cgttggataa aaacgtgatc tgggattacc cgaaagactt
     2101 tattgcaggc aacggtggag tgcgtaattt tcacggtgaa acttgctggt atccttacct
     2161 gacggatatt tgttcaattt ctgatttatt gcgtgaatat attgacaccc caaaagcgga
     2221 gttgttaaca aaacaattta ccagcgataa atgggggtta gtgaatattc tgcgtgctgc
     2281 tgacaggcga atagggatga ggcgattaga tcagttacgg cgtaaaacgc ataatattgc
     2341 tgcgctaaaa aattattgcc cgccgtagcg aataattata tgcctggtgt ggcttcgtac
     2401 gccggataag acgcggcagg cgtcgcatcc ggcattaaag gaaaatcagc aattaacgtt
     2461 gtgcttcgcc acctaatcct tcaatcaggt tttgaattaa cgctgccagt tcaccagtca
     2521 tcaggatgaa atcggcatca aaacgctggg cgaaatcttc acggtcgata tcttcgtttt
     2581 gatcgcgcag ctcgtcgcag aacttcagac gcttgagcga accatcgtcg cacatcacaa
     2641 actgaatgcg ctgctgccag tcgagcgcca gtttagtcac cacttttccg gcttcaatgt
     2701 gattggtgat ctcttcgctg gtcagatctt gtttcttcgc gcggatcact ccgccatctt
     2761 ccagcaacga tttcagctcg gcttcatcaa gcagctggaa gccctgtgcc gcactaccgg
     2821 aacgtaccca ttcggtcagc gtcagttcaa tcgggtttgc catgctcaat ggtacaaccg
     2881 gtaacgaccc caggctttta cgcagcagtg ccagcgtatc ttccgctttt ttggcactgg
     2941 cgcagtccac cataatcaaa ccgttaaccg tgtcgatcca catcattgtc tggctaaaac
     3001 ggctgaaagc acgcggcaac agagagtgca gcacttcgtc tttcagcgaa tctttttcgg
     3061 ttttcttcag cttacgcgcc tgttccgctt ccagtttggc gattttcgct tccagcgcct
     3121 gtttaatcac cggagacggt aggatttttt cttctttgcg cgcgcagata acaatttgac
     3181 cattggcaac gtgcgttaac gcatcgctgt gcgatcccat cggaggaacc cagcccatct
     3241 tcgccatgtc ctggctgccg catggggtaa atgccatcga ggctagctgt ttttccatct
     3301 cttctgcacg cagcgaaatc tcgcggctaa gacggtaaac cattaaattt ttgaaccaca
     3361 gcatgataat ttccacggcc ttgtcgttaa atttagcggg catgataacg aattgtcggc
     3421 ggccttgcat tgccaatccg gttgtccgtc tctacgctat tgatattgaa aaaaataagg
     3481 agagtaccgt gcgtataggt atcgatttag gcggcaccaa aactgaagtg attgcactgg
     3541 gcgatgcagg ggagcagttg taccgccatc gtctgcccac gccgcgtgat gattaccggc
     3601 agactattga aacgatcgcc acgttggttg atatggcgga gcaggcgacg gggcagcgcg
     3661 gaacggtagg tatgggcatt cctggctcaa tttcgcctta caccggtgtg gtgaagaatg
     3721 ccaattcaac ctggctcaac ggtcagccat tcgataaaga cttaagcgcg aggttgcagc
     3781 gggaagtgcg gctggcaaat gacgccaact gtctggcggt ttcagaagca gtagatggcg
     3841 cggcagcggg agcgcagacg gtatttgccg tgattatcgg cacgggatgc ggcgcgggcg
     3901 tggcattcaa tgggcgggcg catatcggcg gcaatggcac ggcaggtgag tggggacaca
     3961 atccgctacc gtggatggac gaagacgaac tgcgttatcg cgaggaagtc ccttgttatt
     4021 gcggtaaaca aggttgtatt gaaaccttta tttcgggcac gggattcgcg acggattatc
     4081 gtcgtttgag cggacatgcg ctgaaaggca gtgaaattat ccgcctggtt gaagaaagcg
     4141 atccggtagc ggaactggca ttgcgtcgct acgagctgcg gctggcaaaa tcgctggcac
     4201 atgtcgtgaa tattctcgat ccggatgtga ttgtcctggg gggcgggatg agcaatgtag
     4261 accgtttata tcaaacggtt ccgcagttga ttaaacaatt tgtcttcggc ggcgaatgtg
     4321 aaacgccggt gcgtaaggcg aagcacggtg attccagcgg cgtacgcggc gctgcgtggt
     4381 tatggccaca agagtaaaaa acccaggcaa ttggcgcatc atgcctgatg cgacgcttgc
     4441 cgcgtcttat caggcctaca aaatgtgcca gaaccgcgta gggcggataa ggcgttcacg
     4501 ccgcatccgg caataagcaa cgcacaccgt gacagtaatc taccccagtg gtttcgccag
     4561 caccggacta tccgccgctt gctggcgctt atagcgacca tacagcagca acgatgacat
     4621 cgcagcaaac gaaagcaggg cggcaggcag cgccacgtaa ttatatgcca gccccagcgt
     4681 cagcatcata cctccgcaat atgcgccgac ggcgctaccg aggttaaacg ctatttgccc
     4741 acctgcggca cctaataact ctccgccttt ggcattttgc agtaacaata tttgcagagg
     4801 tgctgaaagg gcaaataatc ccgcgcaaca aataaaagca aaaataagcg acgttgtttt
     4861 catgccgccg cagaaaaaga gcatcagcag tgccagtaca attataaagt cagtcactgc
     4921 tgcaatgcgc agtggtgaat aacgtcctga aatcctgcca cttagcatat ttcccagcac
     4981 catccctagc ccaactaaca tcataataaa ggtcatcgcc gtttccgaaa aaccggaaat
     5041 aaacatcatg tatggcttta cgtagctgaa ccaggcaaac acacctgcgt tgccaaacat
     5101 ggtggcggcg aaaattaacc acggggccgg gctgcgcaaa aagtgaaatt gttcgcgcag
     5161 ctttcctttc gcctcgtcgc gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc
     5221 aatattaaaa acagcgatca ataaaaaggt gtaacgccag ctaaattcct gacttaaata
     5281 cgttcccagc ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac
     5341 ggcggcggtg acttttccgg gtttgataat ttttgataac acgatcgctc cgacgccaaa
     5401 aaatgcgcca tgcggaaagc cggaaaccag gcgaccaatg gcaagcatca ggtacgacga
     5461 agagagcgtg aacatggcgt tgccaatgac gcacaacgcc accagaaaca acaagatatg
     5521 tttgagtgag tagcggctgg aaaagagtgc gattattggc gcaccgacca ccacccccag
     5581 cgcataatac gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag
     5641 ctccgtgagc acgcccataa tgccaaattc ggccatcccc aaaccaaacg tgcccagagc
     5701 caaagataaa atgacttttt tcataccacc atcacaacta ccagaacggc taccaatcac
     5761 cgtagccaat aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc
     5821 ccctctgtat tcattatcct gctgaatagt tatttcactg caaacgtact ttccagtttg
     5881 ctgtagccca ggccgttgat cttcttcact ttgatctgca caggaatacg ctctttcatc
     5941 gcttcaacgt ggctaatcac accgatggtt ttgccactgg cgttcagggc atccagcgca
     6001 tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc
     6061 gagtcaatac gtgttttatg gctgaccaga tccgaaagcg ccagcgccag cgccagacta
     6121 acgaggaaac tttcgccgcc ggaaagggta cgggtatcgc gtaccgcatc tgcctgccag
     6181 gtatcaacaa cctcgacttc cagcgcctcg ctggctttgc gctgtaacag atagcgcccg
     6241 tgcagccggg taagttgctg attagcgaga tggactaaat tatccagcgt cagcccctgg
     6301 gcaaacttgc ggaatttatc gccctctttg gaacctatta gcgaattcag atatccccag
     6361 tcctcaacct gctgcgtcat ttgagcaatt tgctgcagta aggtttgttg ttgctgacgg
     6421 ttatctgcat cctgcttcag ctgctggcga atctcgcctt gactcgtggt gttttcacgc
     6481 aacttttggt gagtttgcgc taactcttgc tgaatctgct ccaccgtcac agtgagagcc
     6541 aacccgtcag gtcggtgttg ttgatgctgt gccagcgttt ctgctgtctg agtgaccagc
     6601 gtttgcgcct gacggcgctg gttttccaga ttctgcttga gctgttccag ctgcgttagt
     6661 gtttgttcat ccattagcgc cgcaaggaac gcctgctgat cgtcaaagac gctggcctgt
     6721 agcgcggtgt caaactgcgc ctgggctttt tgcagacttt gcgccgccag aacatcctgt
     6781 tgctgtaacg tctgctgctg gctgtgtaat gcgagacatt gttcatgaac ctgccgccag
     6841 ttatccagcg ccacagtttc ttcgctgtgc gggagatcat cactttgcgg caacgtttcc
     6901 agaatcggcg tcagctgctg aatacggttt tgcagcgcgg ttaattcgtt ctggcgttgc
     6961 tgccagctct gcgcttcttg ctgacgtgtc gccaaccagc tctcttcttc atcttcctgt
     7021 ggcaatgtca gtgcataacc cgccaatgcc gttaagagct gctgctggcg ttgttcaatt
     7081 tgctgttgat actggataat ttgctgatta tgcgcggcaa tctgcccttg taattcatgt
     7141 cgttggctga gtaaccgcag ctggcgttcg tgctcatctt gtgcatccag ccacggttga
     7201 atatcgtcct gtggctgcaa ggtgatattg aggctggccg tgacggcttg ccattgttga
     7261 gtaagtgctt gctcatcttg tcggaggctt tgcgcttcgt tttcatcgcg ctgaagctgc
     7321 ttcgttaatg catccagttg cccgcgcagc gccgcacctt cttcgccgag ctttttaact
     7381 tcgttttcca gcgccagtaa tcgagactga ttaacgccag gctccagcgc ctgatacgcc
     7441 tcgaccgccg gatggctggt ggaaccacaa agtggacaag gctgacccgc ctgtaactgt
     7501 gcacgttgag cttccagcgt tttgatgcgc gcttcctgct cgcaaatggt tttcacatcg
     7561 gcaagttgct gcgtcttttc tttataacgc tggcgcattt cgttaagtgc ggcattacgt
     7621 tgcgtctgtt ccagagtgac attctggata gtgaccatta actgcgccag acgtttttgt
     7681 tggggaacaa tctgcccatg cagcgcgacc aggcgctgac gcagtgggcg ttgctcagca
     7741 tgttgcgcca gggcggtagc aacttcatcg gcggttaaca tcaacgtgat cgccgcaagc
     7801 gcattaagtt tttgctcagc atgggttaac tgttgctgcc attgccgcag atgctcgcga
     7861 tcgctggttt gttgggagaa ctgcgcacgc caacccgcca gttcgttgtt ccactgacgg
     7921 aagcggtcgt gttcctgtaa ccaggtattc aggctttgtt gctgctgctg taattctgct
     7981 gactgcttcg ccgcgtggtg gcgaatgctc gcgcgaagcg ccattgtgtt ctgtaagcga
     8041 gtatttactt cttcaatctg ctggcgagta tgcgccagcg ccgcgctgtg ttctgcgatg
     8101 cgttcccagt gcggacgaag atttcgtgcc ggttgtgcca gactgagcgc cgccagttga
     8161 ggttgcgctt tttcttcttc ggctaacgcc tgttgcaagg cctgctgacg gcggctggct
     8221 tcttgctgca attcgtccag acgcgttaac cagtttagtg attgttgttc ttgctgctgc
     8281 gcggtaagta actgtttttc ttcgtcagta agtacctgca aactcgctgt cagcgattgc
     8341 acttgttccg gcgtgagcaa cgcgacgccg ctggcctgcg cttgcagctt ctccagctct
     8401 gtgcgggccg atttgtgctg ctcaaaaacc atcgccgaga tttgcccgta gatttcagtg
     8461 ccggttaact cctcgagcaa ttccgcgcgt tctttgggtt tggcattcag gaaggcagca
     8521 aattgcccct gcgaaagcag catcgaacgg gtgaagcgcc cgtaatccag cccggttaac
     8581 gtcgctgtca gttccagctt atctttcact ttgtcggcga gaattttgcc gtcggcgcag
     8641 cgcgccagct ctacgcgtgg cacctgcaaa ttaccgtcgg gttggttacg cgcccgattc
     8701 tggctccaga acgcgcgata cgcttcacct ttcacttcaa actccacctc cgccagacat
     8761 tcagcggtat cgcgggtcat gagatcattt tgcgattgtg aaacgttaga gagacgcgga
     8821 gtttcgtgat acagcgccag acaaatggcg tccagcaggg tggttttccc cgcaccggtc
     8881 gggccggtaa tggcaaacag cccgttgctg gcgaacggct cgcgggtgaa atcaatcttc
     8941 cattcgcctt ttaatgagtt caggtttttc aggcgcaggc tgagaatttt catgcttcgt
     9001 gttctccggc gagggtatgc aacgtcgtgg tgaaaagatg ctgcagacgt tgctgctgcg
     9061 attcatccag ttcttccagt gccagacggc gattgaacac ctcttcgacg ctgagttcgc
     9121 tgagggtttc acgctgttgg ctggctaaca cacgctcgcg ctgttcacga ctccgacgta
     9181 ccagcaatac ttcaacaggc aatgattcgg ttaatgcctg gattttgcgc tgaatatcat
     9241 gcagatactc atcagtagtg atttcgatat ccagccagac aggtggctcc tgcgatacat
     9301 cgcgccactg ttccagctgt gcggtaatcg acgccagatc gcctttcagc actgccatgg
     9361 gttgcgttac cggtacgttc aggttttcca cgctctctaa tttgccgttt gaaaatgtca
     9421 ccagatggac atatttactc ttaccgcatt catcaaaact cagtggaatg ggggagccgc
     9481 aatagcgaac atgttccatg ccgccaataa tctgtgcgcg gtgaatatgc ccgagcgcga
     9541 tgtagtcggc tggtggaaag ttttgtgccg gaaacgcgtc cagcgtgcca atataaatgt
     9601 cacgcacggc gtcactttta ctggccccca cggtcgttaa atgtcccgtg gcgatgatgg
     9661 gcagaggctg atcgccgcgc agtttgcagg catcggcata gtgttgttgg taataatcgg
     9721 taatcgctgc cagtaaatgc tgctgttttt caataccgtt aagccccgcc tggctggtaa
     9781 taatgtcacg cggacgtaaa aacggaatgg ggcacagcac tgcgcctggc gtcccgtcgc
     9841 gacgaggcaa gatttgcggc gcatgtccgg cgctggcgac cacggtagta ttgaggaacg
     9901 ccatgatatc gcgcgattca ttcagcgtgg cgaccgagtc atggtttcct gccagtacca
     9961 ccagatgaca gccagtttgc tgtaaattga caacaaaacg gttgtataac gtgcgggcgt
    10021 aactgggcgg cgagccggta tcgaaaacat caccggcaac aataatcgca tccacctgat
    10081 gggtttgtgc tgtctccagc agccagtcaa gaaaagcctg atgttcagct tcgcggcttt
    10141 tactgtagaa gttctggccg agatgccagt ctgaggtgtg aaggatgcgc ataacggttc
    10201 cctggcgaaa aagcatgggc gcgattatac ccaaacagat gtgccatttg cttttttctg
    10261 cgccacggaa atcaataacc tgaagatatg tgcgacgagc ttttcataaa tctgtcataa
    10321 atctgacgca taatgacgtc gcattaatga tcgcaaccta tttattacaa cagggcaaat
    10381 catggcgaga cgtattctgg tcgtagaaga tgaagctcca attcgcgaaa tggtctgctt
    10441 cgtgctcgaa caaaatggct ttcagccggt cgaagcggaa gattatgaca gtgctgtgaa
    10501 tcaactgaat gaaccctggc cggatttaat tctccttgac tggatgttac ctggcggctc
    10561 cggtatccag ttcatcaaac acctcaagcg cgagtcgatg acccgggata ttccagtggt
    10621 gatgttgacc gccagagggg aagaagaaga tcgcgtgcgc ggccttgaaa ccggcgcgga
    10681 tgactatatc accaagccgt tttcgccgaa ggagctggtg gcgcgaatca aagcggtaat
    10741 gcgccgtatt tcgccaatgg cggtggaaga ggtgattgag atgcagggat taagtctcga
    10801 cccgacatct caccgagtga tggcgggcga agagccgctg gagatggggc cgacagaatt
    10861 taaactgctg cactttttta tgacgcatcc tgagcgcgtg tacagccgcg agcagttgtt
    10921 aaaccacgtc tggggaacta acgtgtatgt ggaagaccgc acggtcgatg tccacattcg
    10981 tcgcctgcgt aaagcactgg agcccggcgg gcatgaccgc atggtgcaga ccgtgcgcgg
    11041 tacaggatat cgtttttcaa cccgctttta acgccttgct catcggacgc agagcagggc
    11101 ttatgatttc ttaactggag tatcttacgt gctggaacgg ctgtcgtgga aaaggctggt
    11161 gctggagctg ctactttgct gcctcccggc tttcatcctg ggtgcatttt ttggttacct
    11221 gccctggttt ttgctggcat cggtaacagg actgcttatc tggcatttct ggaatttatt
    11281 gcgcctttca tggtggctgt gggtggatcg cagtatgacc ccgccaccgg ggcgtggtag
    11341 ctgggaaccg ctactatacg gcttacacca gatgcagctg cgaaataaaa aacgccgccg
    11401 tgaactgggc aatctgatta aacgctttcg tagcggcgcg gagtcgctgc ccgacgcggt
    11461 ggtgctgacc acggaagagg gcggtatttt ctggtgtaac ggtctggcgc aacagattct
    11521 tggtttgcgc tggccggaag ataacgggca gaacatcctt aacctgctgc gttacccgga
    11581 gtttacgcaa tatttgaaaa cgcgtgattt ttctcgcccg ctcaatctgg tgctcaacac
    11641 cgggcggcat ctggaaattc gcgtcatgcc ttatacccac aaacagttgc tgatggtggc
    11701 gcgtgatgtc acgcaaatgc atcaactgga aggggcgcgg cgtaactttt ttgccaacgt
    11761 gagccatgag ttacgtacgc cattgaccgt gttacagggt tacctggaga tgatggatga
    11821 gcagccgctg gaaggcgcgg tacgcgaaaa agcgttgcac accatgcgcg agcagaccca
    11881 gcggatggaa gggctggtga agcagttgct aacgctgtcg aaaattgaag ccgcaccgac
    11941 gcaattgctc aatgaaaagg ttgatgtgcc gatgatgctg cgcgttgttg agcgcgaggc
    12001 tcagactctg agtcagaaaa aacagacatt taccttcgag atagataacg gcctcaaggt
    12061 gtctggcaac gaagatcagc tacgcagtgt gatttcgaac ctggtctata acgccgtgaa
    12121 tcatacgccg gaaggcacgc atatcaccgt acgctggcag cgagtgccgc acggtgccga
    12181 atttagcgtt gaagataacg gaccgggcat tgcaccggag catattccgc gcctgaccga
    12241 gcgtttttat cgcgttgata aagcgcgttc ccggcaaacc ggcggtagcg gattagggtt
    12301 agcgatcgtg aaacatgcgg tgaatcatca cgaaagtcgc ctgaatattg agagtacagt
    12361 aggaaaagga acacgtttca gttttgttat cccggaacgt ttaattgtca aaaacagcga
    12421 ttaatccgcc tttgtcatct tttattgcca taagccagcc gatgctggct tattttcttt
    12481 gcagtcaaaa tacgggcgtt agattttaca acggttggtg attttttgtt cgcatgatta
    12541 gccatgtctt tttcacggaa atagtgtttt atactggttg gtgatttctt atcgctatat
    12601 acctctggtt tttagatccc tccttgcttt aaaacgttat aagcgtttaa attgcgcttc
    12661 aggtgctgtc atactgactg cattaacgcg gtaaatcgaa aaactattct tcgccgcgcc
    12721 tggttgggag tatttcccgc taaaattgtt taaatatacc gctgtatcat ccccagggat
    12781 tggcacaaaa atttaacgtt acaacaccac atccacaggc agtatgattt atgacccatc
    12841 aattaagatc gcgcgatatc atcgctctgg gctttatgac atttgcgttg ttcgtcggcg
    12901 caggtaacat tattttccct ccaatggtcg gcttacaggc aggcgaacac gtctggactg
    12961 cggcattcgg cttcctcatt actgccgttg gcctgccggt attaacggta gtggcgctgg
    13021 caaaagttgg cggcggtgtt gacagcctca gcacgccaat cggtaaagtc gctggcgtac
    13081 tgctggcaac ggtttgttac ctggcggtgg ggccgctttt cgctacgccg cgtacagcta
    13141 ccgtttcttt tgaagtgggg attgcgccgc tgacgggtga ttccgcgctg ccgctgttta
    13201 tctacagcct ggtctatttc gctatcgtta ttctggtttc gctctatccg ggcaagctgc
    13261 tggataccgt gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc atcctgtctg
    13321 ttgccgcgat tgtctggccg gcgggttcta tcagcacggc gactgaggct tatcaaaacg
    13381 ctgcgttttc taacggcttc gtcaacggct atctgaccat ggatacgctg ggcgcaatgg
    13441 tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa gcgcgcctac
    13501 tgacccgtta taccgtctgg gctggcctga tggcgggtgt tggtctgact ctgctgtacc
    13561 tggcgctgtt ccgtctgggt tcagacagcg cgtcgctggt cgatcagtct gcaaacggcg
    13621 ctgctattct gcatgcttac gttcagcaca cctttggcgg cggcggtagc ttcctgctgg
    13681 cggcgttaat cttcatcgcc tgcctggtaa cggcagttgg cctgacctgt gcttgtgcag
    13741 aattctttgc ccagtacgta ccgctctctt atcgtacgct ggtgtttatc ctcggcggct
    13801 tctcgatggt ggtttctaac ctcggcttaa gccagctgat ccagatctcc gtaccggtgc
    13861 tgaccgctat ttatccgccg tgtatcgcac tggttgtatt aagttttaca cgctcatggt
    13921 ggcataattc gtcccgcgtg attgctccgc cgatgtttat cagcctgctt tttggtattc
    13981 tcgacgggat caaagcatct gcattcagcg atatcttacc gtcctgggcg cagcgtttac
    14041 cgctggccga acaaggtctg gcgtggttaa tgccaacagt ggtgatggtg gttctggcca
    14101 ttatctggga tcgcgcggca ggtcgtcagg tgacctccag cgctcactaa atcactgaac
    14161 atttgtttta accacggggc tgcgatgccc cgtggttttt tattgtgttg atgggttagg
    14221 aattgatgga aagtaagaac aagctaaagc gtgggctaag tacccgccac atacgcttta
    14281 tggcactggg ttcagcaatt ggcaccgggc tgttttacgg ttcggcagac gccatcaaaa
    14341 tggccggtcc gagcgtgttg ttggcctata ttatcggtgg tatcgcggcg tatatcatta
    14401 tgcgtgcgct gggggaaatg tcggtacata acccggccgc cagctctttc tcgcgttatg
    14461 cgcaggaaaa cctcggcccg ctggcaggtt acattaccgg ctggacctac tgctttgaaa
    14521 tccttattgt cgccatcgcc gatgtgaccg cttttggtat ctatatgggt gtctggttcc
    14581 cgacggtgcc gcactggatt tgggtactga gcgtggtgct gatcatttgc gccgtaaacc
    14641 tgatgagcgt gaaggtattc ggtgagctgg aattctggtt ctcgttcttt aaagtcgcca
    14701 ccatcatcat catgattgtc gccggtttcg gcatcatcat ctgggggatt ggcaacggcg
    14761 ggcaaccgac cggtattcat aacctgtgga gcaacggcgg cttcttcagt aacggctggc
    14821 ttggcatggt aatgtcgttg caaatggtga tgtttgctta cggtgggatc gaaattatcg
    14881 ggattaccgc cggtgaagcg aaagatcctg agaaatcgat accgcgtgcg attaactccg
    14941 tgccgatgcg tattctggtg ttctacgtcg gtacgctgtt cgtcattatg tctatctacc
    15001 cgtggaatca ggttggcact gccggtagcc cgttcgtgct gacgttccag catatgggca
    15061 ttacctttgc cgccagcatt cttaactttg ttgtgctgac tgcttcgctg tcggcaatta
    15121 acagtgatgt atttggcgta ggccgtatgc tccacggtat ggcagagcag ggcagcgcgc
    15181 cgaaaatttt cagcaaaacg tcgcgtcgcg gtattccgtg ggttacggtg ctggtgatga
    15241 ctaccgcgct gctgtttgcg gtgtatctga actacatcat gccggaaaac gtcttcctgg
    15301 tgattgcttc gctggcaacc ttcgccacgg tgtgggtgtg gattatgatc ctgctgtcgc
    15361 aaattgcctt ccgtcgccgt ttgccgccag aagaagttaa ggcgctgaaa tttaaagtgc
    15421 cgggtggggt agcaacgacc atcggcgggc tgattttcct gctctttatt atcgggttga
    15481 ttggttatca cccggatacg cgtatctcgc tgtatgtcgg tttcgcgtgg attgttgtgc
    15541 tgttgattgg ctggatgttt aagcgtcgcc acgatcgtca gctggctgaa aaccaataat
    15601 acttgcgttc tgtaagccgg ttcaggagga aatcctgatc cggctattcc gaaagttatc
    15661 cgcccccgtc ctcctccccc aaatatcctt cagatgatga gtgatcctgc attaggctat
    15721 ggcaaggtga tcagattttc atcacagggg aattatgatg ttaaatgcat ggcacctgcc
    15781 ggtgccccca tttgttaaac aaagcaaaga tcaactgctc attacactgt ggctgacggg
    15841 cgaagaccca ccgcagcgca ttatgctgcg tacagaacac gataacgaag aaatgtcagt
    15901 accaatgcat aagcagcgca gtcagccgca gccaggcgtc accgcatggc gtgcggcgat
    15961 tgatctctcc agcggacaac cccggcggcg ttacagtttc aaactgctgt ggcacgatcg
    16021 ccagcgttgg tttacaccgc agggcttcag ccgaatgccg ccggcacgac tggagcagtt
    16081 tgccgtcgat gtaccggata tcggcccaca atgggctgcg gatcagattt tttatcagat
    16141 cttccctgat cgttttgcgc gtagtcttcc tcgtgaagct gaacaggatc atgtctatta
    16201 ccatcatgca gccggacaag agatcatctt gcgtgactgg gatgaaccgg tcacggcgca
    16261 ggcgggcgga tcaacgttct atggcggcga tctggacggg ataagcgaaa aactgccgta
    16321 tctgaaaaag cttggcgtga cggcgctgta tctcaatccg gtgtttaaag ctcccagcgt
    16381 acataaatac gataccgagg attatcgcca tgtcgatccg cagtttggcg gtgatggggc
    16441 gttgctgcgc ttacgccaca atacgcagca gctgggaatg cggctggtgc tggatggcgt
    16501 gtttaaccac agtggcgatt cccatgcctg gtttgacagg cacaatcgtg gcacgggggg
    16561 agcttgtcac aaccccgaat cgccctggcg cgactggtac tcgtttagtg atgatggcac
    16621 ggcgctcgac tggcttggct atgccagctt gccgaagctg gattatcagt cggaaagtct
    16681 ggtgaatgaa atttatcgcg gggaagacag tattgtccgc cactggctga aagcgccgtg
    16741 gaatatggac ggctggcggc tggatgtggt gcatatgctg ggggaagcgg gtggggcgcg
    16801 caataatttg cagcacgttg ctgggatcac cgaagcggcg aaagaaaccc agccggaagc
    16861 gtatatagtt ggagagcatt ttggcgatgc acggcaatgg ttacaggccg atgtggaaga
    16921 tgccgccatg aactatcgcg gcttcacatt cccgttgtgg ggatttcttg ccaataccga
    16981 catctcttac gatccgcagc aaattgacgc tcaaacctgt atggcctgga tggataatta
    17041 ccgcgcaggg ctttctcatc aacagcaatt acgtatgttt aatcagctcg acagccacga
    17101 tactgcgcga tttaaaacgc tgctcggtcg ggaggttgcg cgcctgcccc tggcagtggt
    17161 ctggctgttc acctggcctg gtgtaccgtg catttattac ggtgatgaag tgggactgga
    17221 tggcaaaaat gatccgtttt gccgtaagcc gttcccctgg caggtggaaa aacaggatat
    17281 ggcgttattc gcgttgtacc agcgaatgat tgcgctgcgt aagaaaagtc aggcgctgcg
    17341 tcgtggcggc tgtcaggtgc tgtatgcgga agataacgtg gtggtatttg tccgcgtgct
    17401 gaatcagcag cgtgtactgg tggcaattaa ccgtggcgaa gcctgtgaag tggtgctacc
    17461 cgcgtcaccg tttcttaatg tcgtgcaatg gcaacgcaaa gaaggccatg ggcaactgac
    17521 agacgggatt ctggctttgc ctgccatttc ggctacggtg tggatgaact aaagcatctg
    17581 ttttatttgt tacgtcgtta gtcaccagcc ctgacactac gcagcgcaaa aaagctgctc
    17641 aatgtcaggg ctgaatttgt taaatatttg tcccgatcga tatgagcgat ttttggtatt
    17701 gtttgcccat gatgttatgt acaacatgct ccaggataac cctgtagtgg agcaaagata
    17761 tgacaatttc aaattcgaat ttattgaaac cgtttattac tggtactga
//
LOCUS       DBFIFS010000078        17807 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.078, whole genome shotgun sequence.
ACCESSION   DBFIFS010000078 DBFIFS010000000
VERSION     DBFIFS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.078"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(102..1694)
                     /gene="lsrK"
                     /locus_tag="N7A07_002376"
     CDS             complement(102..1694)
                     /gene="lsrK"
                     /locus_tag="N7A07_002376"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU2477030.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1773..2726)
                     /gene="lsrR"
                     /locus_tag="N7A07_002377"
     CDS             complement(1773..2726)
                     /gene="lsrR"
                     /locus_tag="N7A07_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU2477031.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2975..4510
                     /gene="lsrA"
                     /locus_tag="N7A07_002378"
     CDS             2975..4510
                     /gene="lsrA"
                     /locus_tag="N7A07_002378"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU2477032.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4504..5532
                     /gene="lsrC"
                     /locus_tag="N7A07_002379"
     CDS             4504..5532
                     /gene="lsrC"
                     /locus_tag="N7A07_002379"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU2477033.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5532..6524
                     /gene="lsrD"
                     /locus_tag="N7A07_002380"
     CDS             5532..6524
                     /gene="lsrD"
                     /locus_tag="N7A07_002380"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU2477034.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6536..7558
                     /gene="lsrB"
                     /locus_tag="N7A07_002381"
     CDS             6536..7558
                     /gene="lsrB"
                     /locus_tag="N7A07_002381"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU2477035.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7585..8460
                     /gene="lsrF"
                     /locus_tag="N7A07_002382"
     CDS             7585..8460
                     /gene="lsrF"
                     /locus_tag="N7A07_002382"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU2477036.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8484..8774
                     /gene="lsrG"
                     /locus_tag="N7A07_002383"
     CDS             8484..8774
                     /gene="lsrG"
                     /locus_tag="N7A07_002383"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU2477037.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8831..9589
                     /gene="tam"
                     /locus_tag="N7A07_002384"
     CDS             8831..9589
                     /gene="tam"
                     /locus_tag="N7A07_002384"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU2477038.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9593..10507)
                     /gene="yneE"
                     /locus_tag="N7A07_002385"
     CDS             complement(9593..10507)
                     /gene="yneE"
                     /locus_tag="N7A07_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU2477039.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10704..12155)
                     /gene="uxaB"
                     /locus_tag="N7A07_002386"
     CDS             complement(10704..12155)
                     /gene="uxaB"
                     /locus_tag="N7A07_002386"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU2477040.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12382..13800)
                     /locus_tag="N7A07_002387"
     CDS             complement(12382..13800)
                     /locus_tag="N7A07_002387"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2477041.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13939..14298)
                     /gene="yneG"
                     /locus_tag="N7A07_002388"
     CDS             complement(13939..14298)
                     /gene="yneG"
                     /locus_tag="N7A07_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU2477042.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14298..15224)
                     /gene="glsB"
                     /locus_tag="N7A07_002389"
     CDS             complement(14298..15224)
                     /gene="glsB"
                     /locus_tag="N7A07_002389"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU2477043.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15288..16676)
                     /gene="sad"
                     /locus_tag="N7A07_002390"
     CDS             complement(15288..16676)
                     /gene="sad"
                     /locus_tag="N7A07_002390"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU2477044.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16777..17658
                     /gene="yneJ"
                     /locus_tag="N7A07_002391"
     CDS             16777..17658
                     /gene="yneJ"
                     /locus_tag="N7A07_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2477045.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17736..>17807
                     /locus_tag="N7A07_002392"
     CDS             17736..>17807
                     /locus_tag="N7A07_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477046.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAP"
BASE COUNT         4442 a         4553 c         4452 g         4360 t
ORIGIN      
        1 caagctggca tttaacaagc cagctctaaa agaagggaaa taagaataac tatactcaaa
       61 aactaacagc cacggtcatc atgatgtggc tgtcaatgaa actataaccc aggcgctttc
      121 cataacgacg tcgtcagtcc atgatcaacc agccccagct gatcctgata aactgcctgc
      181 cacttatcgc gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt
      241 tcccagcgaa ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg
      301 ccagctgcaa tggcacatcc taatgcagtg gcttctttga ccaccggaat attgacgggt
      361 aatcccgaga catcagcgag aatttgactc cataatttcc cttttgaacc tccgcctgca
      421 aagactaacg atgaaggatg aatagtcgaa aaatcagcaa tttgctgcaa gttacacgct
      481 gatacaatcg ccgcattttc ttccagcgca cggaacaatg tcgctttgtt acatttatcc
      541 ggatcaatgg acaaattaat aaaggaaggc gcagcgtgat accaggtttt aaagcgcatt
      601 ctgtcggaga agatcggcat tacgccccac gacccaggcg gcacccgact ggccatctct
      661 tccagcagcg tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag
      721 aaagcatcgc ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt
      781 accatgccag gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc
      841 gcggctaaat ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt ttgtgccgga
      901 cgcacaacgc ctaaccccag gcaaccaagc tgcacgtcgc ctcctccaac gaccaccgga
      961 gtgcccacct tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc cagcaatgtg
     1021 ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc cagcaatgca
     1081 ggtttccagt cacggatggt tagatcaaga agtcccgtgg tgccagcgtt agagggatcc
     1141 accgcaagtt cgccgctgag catataggcc agccagtcgc tgatcatggt gatggttgat
     1201 gcctgacggt aaatatcgga acgatggtgc gccagccaaa gtaatctggg gatggcactt
     1261 aaagccagtg tttgtccggt cgcgcgataa acttcgtttt caaaggtatt gttgtgcagt
     1321 tctttaagtt cgctaacttc gcgcgccgct ctggcatcca cattggcgca ggcccagatc
     1381 ggtgttcctt cattgttata taaaacaatg ccttcacgca tcgaacatgc cgaaacggca
     1441 gcgatatact ccggggctat gccggcgttg tgcagcgcct ggcgcataca ctcacacgcc
     1501 agttgccagt ttttgttgag atcaaattcc atagaaccag gaacgtccgg tactgccaga
     1561 tgccgccact ccgcctgtcc cactgctatt tgattgcctt ccaggtcgaa tatcacagcc
     1621 cgaatacttc cggtgcctgc atccagcgcc atcaggtagt actttgattc tgaaggggta
     1681 aagagtcgag ccattagatt atcctcggct tatgcgttct gtctgttcct ctatacgttc
     1741 tccatcattc ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc cgctgctgtg
     1801 tcctgatcgg taaccagtgc gttgatataa ccgcctttca ttgcagcggc aattgcttcg
     1861 gctttatttt ctccccctgc cacgccaacc cggacgggta tggtcttcag cgcgcttaaa
     1921 ggtaagccaa tcagctcgtt atgtatttta atgtccgtga caacgtcacc ttttgcatca
     1981 aaaaagtagc ctaaaatgtc gccaaccgcc ccttttcggc caatcattaa ctgttcgccc
     2041 tggctgatat aaccggagcg aatgatcgtc gcgtcgtctt gttgactaac ggccccaatg
     2101 ccgacaatcg ccacatccgc tgcttgcgcg gctaacagaa catctttgac acaattttca
     2161 ttttttagcg tacgggcaat gtcagcggag gatgcccgca acggagccgg aataatattc
     2221 acactgcacg ccgcattaag ctgtccgata cccgtcatat aagaaccgac gccaccggag
     2281 agcgtgacca ggcgaatttg ctgtgacgaa ataaaaccac ttaagcgttg cagcgtattc
     2341 atggttgcct cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa actcatcaac
     2401 atatgcgccg cgcctatccc cagtcgcccg ccgacatcag catccgcaag cccagggatc
     2461 acccggacat gttgcagcga aaactgacga cgtaattgtg tttcatattc cagacagcct
     2521 tcaaagcgag aattaatctg cacgcgaatg atgccggact gatgcccttt ctccagtaat
     2581 cgcgacactt tcaaacgggt caggccgaga cgatcgctga tctcactctg ggtcagcccg
     2641 tcgtggtagt aaaaccacgc gatccgcgcg acctgttctt cttcacacat tccctgttct
     2701 gaaattaccg aatcgttgat tgtcataatt cattcttcac tttgaacata tttaaatctt
     2761 taatgcaatt gttcagttct tgcgcattta tatctgtgat ggcaaccaca gtttgactct
     2821 acgagcatga acaaacgcaa ccgtgaaaat caaaatagta taaattgtga tctattcgtc
     2881 ggaaatatgt gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa atacatttgt
     2941 tcaaaactca cctgcaaaac tgaacggggg aaatatgcaa acgagtgata cccgcgcgtt
     3001 accgctactt tgcgcccgct cggtttataa acagtattca ggggtcaatg tcctgaaagg
     3061 catcgatttt acgttgcatc agggggaggt ccacgccctg ctcggcggca atggtgccgg
     3121 taaatcgacg ttaatgaaga ttattgccgg tattacctct gctgatagcg gtacgctgga
     3181 gattgggggc aacaactacg ccagattaac gccagttcat gctcatcagc tgggtattta
     3241 tctcgttccc caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa acatcctgtt
     3301 tgggctggca aaaaaacagc tctccatgca gaaaatgaag aacttgctgg cggcgctggg
     3361 ctgccagttt gatctgcata gtctggcagg atcgctggat gtcgccgatc gccaaatggt
     3421 ggaaatcctc cgcgggctga tgcgcgactc gcggattctg atcctcgatg aacctaccgc
     3481 ctcgcttacc cctgcggaaa ccgaacgctt gtttactcgc ttgcaagagc tgcttgctac
     3541 tggcgtgggt attgttttta tctcgcataa gctgccggaa attcgccaga ttgccgatcg
     3601 gattagcgtg atgcgcgacg gaaccatcgc cttaagcggc aaaaccagcg aactgtctac
     3661 cgacgacatt attcaggcca tcaccccagc ggtacgggaa aaatcgctct ctgccagcca
     3721 aaaattatgg ctggagttac ctggcaaccg cccacaacat gccgcaggaa cgtcggtgct
     3781 gacactggaa aatctgactg gcgaaggttt caggaatgtc agcctgacgc taaatgccgg
     3841 agaaattctg ggtctggctg ggctggtggg tgccggacgc acagaactgg ccgagacgct
     3901 ctatggtcta cgtactttgc gtggcggacg cattatgctg aatgataaag agatcaatag
     3961 attatccact ggagaacgtt tactgcgcgg tctggtttat ctgccggaag atcgccagtc
     4021 atccggactg aatctcgatg cttcgctggc ctggaacgtc tgcgccctta ctcataacct
     4081 tcgtggattc tgggcgaaaa ccgcgaaaga taatgccacc ctggaacttt atcgtcgggc
     4141 gctgaatatt aaatataacc aaccggaaca agctgcacgg acattatccg gtggtaacca
     4201 gcaaaaaatc ctgattgcca aatgcctgga agcctcgccg caagtattga ttgtcgatga
     4261 gccgacgcgc ggcgtggatg tctcggcgcg taatgatatc taccaactgt tgcgcagcat
     4321 cgccgcacag aatgtggctg tgctgcttat ctcctctgat ctggaagaga tcgaactgat
     4381 ggcagatcgt gtgtatgtga tgcatcaggg cgaaattacc cactctgcac tgaccgggcg
     4441 cgatattaat gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc gtcaggaggc
     4501 ggcatgctga agtttattca gaacaaccgt gaaatcacgg cactgctggc ggtgctgctg
     4561 ctgtttgtat tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac gctgaccatg
     4621 gtttatagca gcgcgcaaat cctgatcctg ctggcaatgg gcgcgacgct ggtaatgctt
     4681 acgcgcaata ttgatgtttc agtgggttcg attaccggaa tgtgcgcggt gctgttgggg
     4741 atgttactga acgcaggata ttcactacct gttgcctgtg tcgcgacttt actgcttggt
     4801 ttgctcgcgg gatttttcaa cggtgtcctg gtcgcgtggc taaagatccc tgccattgtt
     4861 gccacccttg gcacgttagg gttgtacaga ggcatcatgt tgctgtggac tggcggtaaa
     4921 tggattgaag ggttacccgc cgaactgaaa cagctctccg ccccgctgct gcttggcgtt
     4981 tcagcaattg gttggttaac gataattctg gtggcattta tggcctggct gctggcgaag
     5041 acagcgtttg gacgcagttt ttatgccacg ggcgataatt tacagggcgc tcgtcaactg
     5101 ggcgttcgta cggaagccat tcgcattgtg gcattttcgt tgaacggctg catggcggca
     5161 ctggcgggaa ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac cggtaccggg
     5221 ctggagatga aagcaattgc agcctgcgtg ctgggcggca ttagtttgct cggcggttcc
     5281 ggtgcgatca tcggtgcggt actcggcgca tggttcctga cgcagatcga tagcgtactg
     5341 gtgctgttgc gcattccggc atggtggaat gattttatcg cgggtctggt tctgctggcg
     5401 gtgctggtgt ttgatggacg cctgcgttgt gcgctggaac ttaatctgcg gcggcaaaaa
     5461 tatgcccgct ttatgacgcc accgccatcc gttaaacccg cttcgtcagg taaaaaacgg
     5521 gaggctgcat aatgcgtatt cgctacggtt gggaactggc tcttgccgca ctgcttgtta
     5581 ttgagattgt ctcatttggt gcaattaacc cgcggatgtt agatctcaat atgttgctgt
     5641 tcagcaccag tgactttatc tgcattggca ttgtcgccct accgctgacg atggtgattg
     5701 tcagtggcgg gatcgatatt tcgtttggtt cgaccatcgg cctctgcgcc attgcattgg
     5761 gcgtactgtt tcaaagtggt gtgccgatgc cgctggcgat tctcctgacc ttactgctcg
     5821 gcgcattgtg cgggctgatc aacgccggat taattatcta taccaaagtt aacccgctgg
     5881 tgattacgct tggcacgctg tatctgtttg ccggaagcgc cctgctgctt tccgggatgg
     5941 ccggggcgac ggggtacgaa ggtattggtg gattcccgat ggcgtttaca gatttcgcta
     6001 acctggatgt gttggggctc cccgttccgc tgattatctt cctgatatgt ctcctcgttt
     6061 tctggctctg gctgcataaa acccatgccg gacgtaacgt gtttttgatt gggcaaagcc
     6121 cgcgcgtggc gctttatagc gcgattccag ttaaccgtac cttatgtgcg ctctatgcca
     6181 tgacggggct ggcgtctgcg gtcgccgctg tgctgctggt gtcgtatttt ggttcagcac
     6241 gttccgatct cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg cttggcggtg
     6301 ccaatattta tggtggttcc ggtgccatta tcggcaccgc cattgcggtt ttattagtgg
     6361 gatatttgca acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc agcgcccttt
     6421 ccggtgcgct acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat cgccagcaaa
     6481 ttaaagagtg gctggcgcgt cgggccaata acccattgcc ataaaggata tcttcatgac
     6541 acttcatcgc tttaagaaaa tcgccttact tagcgctctt ggcattgccg caatctctat
     6601 gaatgtgcag gccgcagagc gtattgcatt tattcccaaa ctggttggcg tgggattttt
     6661 taccagcggt ggcaacggcg cacaacaagc gggtaaagag ctgggcgttg atgtgaccta
     6721 cgacgggccg acagaaccca gtgtttctgg tcaggtacag ttgattaata acttcgtcaa
     6781 tcaaggttat aacgccatta tcgtttctgc ggtttcgcct gatggcttgt gtccggcact
     6841 gaaacgcgcc atgcaacgtg gtgtgagagt gctgacctgg gactctgata ctaaaccgga
     6901 gtgccgctct tactacatta atcagggaac tcccgcccag ttgggaggta tgttggtgga
     6961 tatggcggcg cgtcaggtga ataaagacaa agccaaagtc gcgtttttct actcaagccc
     7021 caccgttacg gaccaaaacc agtgggtgaa agaagcgaaa gcgaaaatcg ccaaagagca
     7081 tcctggctgg gaaattgtca ctacgcagtt tggctataac gatgccacta aatcattaca
     7141 aaccgcagaa ggaatattaa aagcgtatag cgatctcgac gccattatcg cccccgatgc
     7201 caatgccctg cccgctgccg cacaagccgc agaaaacctg aaaaatgaca aagttgcgat
     7261 tgtcggattc agtacgccaa acgtgatgcg tccatatgtg gaacgcggca cggtgaaaga
     7321 atttggcctg tgggatgtgg ttcagcaagg caaaatttca gtttatgtcg cggatgcatt
     7381 attgaaaaaa ggatcaatga aaacgggcga caagctggat attcagggcg taggtcaggt
     7441 ggaagtctcg ccaaacagcg tccagggcta tgactacgaa gcggatggta atggcatcgt
     7501 actgttaccg gagcgcgtga tattcaacaa agagaatatc ggcaaatacg atttctgatg
     7561 tgcattactt aaccggagta agttatggca gatttagacg atattaaaga tggtaaagat
     7621 tttcgtaccg atcaaccgca aaaaaatatc ccttttaccc tgaaaggttg cggtgcgctg
     7681 gattggggaa tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg taaaaccgtg
     7741 atgctggctt ttgaccatgg ttattttcag ggaccaacta ccggacttga acgcattgat
     7801 ataaatatcg ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg cgggattttg
     7861 cgcagcgtag ttcccccggc gaccaataag ccggtggtac tgcgggcgtc aggtgcgaac
     7921 tctatcctgg cggaattaag caatgaagcc gtggcgttat cgatggatga cgccgtgcgc
     7981 ctgaacagtt gcgcggtggc ggcgcaggtt tatatcggca gcgaatatga acatcagtcg
     8041 atcaaaaata ttattcagct ggtcgatgcc ggaatgaaag tgggaatgcc gaccatggcc
     8101 gtgactggcg tgggcaaaga catggtgcgc gatcagcgtt atttctcgct cgcgactcga
     8161 atcgccgctg aaatggggac gcaaattatc aaaacctatt atgtcgaaaa aggttttgaa
     8221 cggattgttg ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa attaccggag
     8281 cgcgaggcgc tggaaatgtg ctggcaggct atcgatcagg gcgcttctgg tgtggatatg
     8341 gggcgtaata ttttccagtc tgaccatccg gtggcgatga tgaaagccgt acaggcggtg
     8401 gttcaccata acgaaacggc tgatcgggca tatgaactct atctgagtga aaaacagtaa
     8461 ctgcggattt aaggagaaga attatgcacg tcacactggt tgaaattaac gttcatgaag
     8521 acaaggttga cgagtttatc gaagtttttc gccagaacca cctgggctct gtacaggaag
     8581 aaggcaattt gcgcttcgat gtcttacagg acccggaagt gaattcgcgc ttttatatct
     8641 acgaagccta taaagatgaa gacgcagtgg cgttccataa aaccacgccc cactacaaaa
     8701 cctgtgtcgc gaaactggaa tctttaatga ccgggccgcg taaaaaacgt ctgttcaatg
     8761 ggttgatgcc gtgaggcgaa tttatcagtt ttatctacaa ttggggtaac gcgctgacgg
     8821 gagtaaataa atgtctgact ggaacccctc tttatatctg cactttgccg ctgaacgatc
     8881 gcgtccggcg gtggagctgc ttgccagagt gccgctggag aatgtcgatt atgttgccga
     8941 tcttggctgt ggcccaggta acagcaccgc cctgctgcat caacgttggc ctgctgccag
     9001 gataacgggc atcgactcgt ctccggcgat gattgctgaa gcgcgcagtg ctttgccaga
     9061 ctgccagttc gtggaagcag acatccgcaa ctggcaaccg gaacaggcac tcgatctgat
     9121 ttttgctaat gcctcactgc aatggctgcc cgaccactac gaattgtttc ctcacttggt
     9181 ttctttactt aatccgcagg gcgtgctggc agtacagatg ccagataact ggctggagcc
     9241 gacccatgtg ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc gcgggcgtga
     9301 gtcgttggct ggtgttcatg cttactacga tattttgagc gaagccggat gtgaggtcga
     9361 tatctggcga accacctact atcaccagat gccgtcgcac caggcgatta tcgattgggt
     9421 gaccgccact ggattacgtc cgtggttaca ggatctgacc gagagcgaac agcagctttt
     9481 tcttacgcgc taccatcaga tgctgaaaga gcagtatcca ctgcaagaga acggacagat
     9541 actgctggca tttccgcgtc tgtttattgt tgcccgccgt acggagtaaa tatcacgtca
     9601 gttggtaatg gcgatcggga agaacttttg ctggaatttc ggcttcatcg ttcatctgta
     9661 agaggtcaat ttcaatagcg ttacagatgg catccagcgg taaatcattg ttttcagtac
     9721 cgaacggatc ttccagttct tccgccagac aatccagcga aataaaagta taggaaatca
     9781 gcacagagat aaaaggcgtc atgtaatgca ggtccacgac cagcgcgaac ggcagcatga
     9841 tacaaaacag ataaacggta cgatgcaaaa tcagggtgta ggcaaaggga attggcgtgt
     9901 aggcaatgcg ctcgcagcct gccagaaccg ctgaaatatc gttaagccga tcgttgaggc
     9961 taataaacag gatatctgaa agttgtccat tgcgtcgctg aaccgccaac cactccccca
    10021 ttattaacaa gatgcggtta gctggagagt tcgaagccag tacacgctga agatcttcag
    10081 tcttgagata atgagccagc acttccacct gtggctgttt gcgtaatgtc atgcgtaaac
    10141 agtgggcgaa ggcgatttgc agccgggcaa actcccttac acttgccgaa tccggcaatg
    10201 tcgtttttac ctcgcgcagt aacgaccgtg aggcaatcat caactgcccc caaagtttgc
    10261 gcgcttcaac gtaacgggcg taaccggcat tattacgaaa accaagaaaa atggcgatgg
    10321 cgacaccgag aatgctgaac ggtgcgaggg tgaatttgat gcccagatgc gtgtaccagg
    10381 gcagcatgaa aataacagcg atagaaaaga gaaaattgag tagtaagcgc gaggatatct
    10441 tggataatac tgagccatgc cagacaaaaa tacggcgcag ccagtgttgt tgtggacgaa
    10501 caatcatggt tatcttcagg cgtggaaaag tcgccctatt aaacgtgatt acgatcacat
    10561 tctcaagaca ctacttacaa attacctaca aaattacaac attaacaggt gctgcatgtg
    10621 gcacctgtta atgataaagg cttataggat gttgtaacta atataatgaa ataagaaacc
    10681 cggtcgaaac cgggttcaga gtattagcac aacggacgta cagcttcgcg catccctttt
    10741 tcgagaatcg catccaggtc attagccacc tgctcgacca gaccaggcac ttgcgtcagg
    10801 tcctgctccc agtggtcttt ttctgccaac acaatcgcta ccagttcctg ggtaccgata
    10861 acacggtcac gatgctgact ccagagttgc tgataacgtt ccagccagtg tgcatcgtcc
    10921 tgtaccggat atgtttctcc attacgctca ccgcgataga acgcaatcaa tgccgctaat
    10981 gcgaaagtaa ggcgcgccgg aagtgtgccg tttgccttct gccctgccag cagctgcggc
    11041 aggatgcggg tacggaactt ggtcataccg ttgagcgcga tagacagcag ctgatgctta
    11101 atgtacgggt tacggaaacg cccggtgact gcactggcga aagattccag ttcatcgcgt
    11161 ggcaaatcca gtaccggaat aatttcttcg tagatggctt tttcaacgaa tgcgcaaatt
    11221 tcagcatcgt tcatcgcttc acctacggta tccagccctg cctggaacgc caccggcacc
    11281 aacgcggtgt gtgcaccgtt gagtattgcc actttgcgtt ctttatacgg tttaatatcg
    11341 tcaacaatca gcacgttgag cggatatttg tccagacgca gctcggtcgc taaggatttc
    11401 ggtccctgaa tcacaaacaa gtaaaagtgt tcagcggtgt cgaggaaacc atcgtgataa
    11461 cccagttctt cttccagttt agccacttca tcacgcggat aaccggtaac gatacggtcc
    11521 accagcgtag aacagaagct gttagcctga tccagccatt gaataaatgc ttctggcagt
    11581 gcccactctt gcgcatagcg cagcaccagt tcacgcaacg cgtcgccatt gtagtcgatc
    11641 aactcacacg ggatgatgat ccaaccttta tccagcgcac cgttgaaatg gctaaaacgt
    11701 tcgaacagca gacgggtcag ttttgccgga taacttaccg ctggcgcgtc atcgaattta
    11761 tcgcccgcat ggtagctgat acccgcttcg gtggtgttag agaaaacaaa gcgcatttcc
    11821 gggttgtgcg ccagtttcag gaattcatcg tattcactgt agacgctgat ttcacgatta
    11881 acagagcgaa tcagacgcgc gtcgctgacc gcttccccct tctcattcag gccacggata
    11941 atggtggtgt acagaccatc ctgcgtgctc agtgacggcg ggaatgaagt ttcaatcgga
    12001 cgaacaacga ccacaccaga attcagatcg gtgtgctcat tcaggagatc tatttgccag
    12061 tcaacaaagg cgcgcaggaa gttaccttca ccaaactgaa tgatacgttc tggatactgt
    12121 gcaccgggaa aatcgcgacg atttagtgtt ttcacaatgg gttcccttct gattagtcat
    12181 acaacctgtt tgaattggta cgacaggtta gcaaacttta atacgacgaa cccctgtttt
    12241 gatcaactcc tgatgattaa tgagcagttt tatgagaaaa gtgtggcgcg gatcatggtt
    12301 taatcgagga aatacgcctt ttcctggatc ataaagtggt agaacacatt gcattcaaat
    12361 cgcgcggaat gaataaagat gtcagacaac ttcctcaccg taacgcatag tgctggtacg
    12421 gttgcgccca tctttcttcg agcgatacag acaggtatcg gcttcgacca tcaatttatt
    12481 aaagtcatcg gtcagcgttc ggtacgatgc gcgaccacta ccgacgccaa tacttaccgt
    12541 gagataaagc gttttttgtt gccaggtgaa tggttgcagt tcaacgcctt tacggatttt
    12601 ttccgccatt agcagaccat cgacaggatt cactgacggc actgcaacag caaattcttc
    12661 gccgcccatg cgcgccacca gccccttatc accgacaatc ttctgaatat gccgggcaaa
    12721 cacgcttaac actttatcgc cacattcatg tccatagtta tcgttaatac ttttgaaata
    12781 gtcgatatca agtagcatga cagtcagatg ttgtgtctgt ttcagcgatg gacttttcag
    12841 cgcctcataa agaccggacc gtgagtaaac ctgagtcaga aaatcaaagt cggctcgcag
    12901 cgcaacttgc ttcattagcg aattgatcgc tgccacgcta aaagaaacca taattgggca
    12961 tatcgccatc gtggcaatac cgagacgtgc ggagaacatt tgcggaatgg agaacggcga
    13021 accgaccgag atatcaatca ccgaattagc caccagtacg atttccaccg caccggtgac
    13081 aaaggtcaac agacatgtta cctgcggcgt atagcgcact gcacaccaga ttaatgcagg
    13141 cagcgggaac gccagactcc ccgctccgcc aatgactacc gaggcaataa ccgacacaat
    13201 aagcgcgatg gcgggcatca tctgttctgc tttaaagcgc ggcagcactc caggaatagc
    13261 caacgtcagc atacaaggca cgatcaacac gcccgttgag aattgctcac tgaaccagtc
    13321 cgcaagcaaa ggccagaaat ccagactatc aatactgacc gaaccaatcg ccccgacaat
    13381 agcgcataat aatgcggcaa tcagacagta attaaatagc cgtaaggcac ttactggctc
    13441 atatttattt ttccccaaac gcttatcccg cacgaccagt aaggcgacgg taacaataaa
    13501 catcatattg gagaaattga tagccagtga aaccagtccc cattcggtgg ttatggcatc
    13561 ataaaccagc atcgccacat aactgatcgc atagtaatgc agtcgattaa gccagacata
    13621 gcgggcaaac acccccgcca ttacgccatt cagcggccag aaaagagaaa gttcgtgaac
    13681 cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt agcgcaaaaa tagcgatgct
    13741 attacgtagc agatgacctt cctggaacac acggaaagtt gaaatggggt gtacatgcat
    13801 taattatctg tccatacaat tgcgggtggc aattatctca acgtaataag taaattctca
    13861 taaaggatag catgttcgct gacagcggcg gcaaataaag gtaccctggg aatgctattt
    13921 tgtcgttttc cgcaattctc atggctgatt catctgtaca accagccagt gatagattac
    13981 cgccacgata taacgttgtt gttcttcgct tagcgaaaca ccatgcggga tgttatgcca
    14041 cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc tgagcgataa acaccggatg
    14101 cccgcgcatg ggggtttgct taccgtcatt agccggtaat gccggagcaa gacgtttagc
    14161 gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct ttctccagac aatactgacg
    14221 ctctttcatg cccaaacgaa agcgagatcg aaattttgaa cgggataaac gcgcgaagag
    14281 tggatcgaga gactgcatta ataaaccgaa cgccctaact gtttcgtcaa ttgttcaagg
    14341 acggcgatac ccgcaagcga gttacctgca tcatccagtt ccggactcca gactgcgatg
    14401 gccatttcat gcggaacaat cgccacaata ccgccaccaa cgccagattt cgccggtagt
    14461 cccacccgcc aggcaaactc ccccgcgttc tggtacatac cactggtcgc catcagcgcg
    14521 ttaatttgcc gtgcctgcat tggcgtcacc acaggttcat caatatgaat agctttcccc
    14581 tgattagcca gaaagacaaa cgtccgggcc agctctacac agctcatttt cagagcgcag
    14641 taatgaaaat agttttgcag aacggttgtc acgtcatgat ggaaattgcc aaacgacttc
    14701 atcagccagg cgatagccgc atttcgcgcg gaatgttcaa attcggaacg cgctaccacc
    14761 gtatcgtagg aaatatcaga cacaccgctt aagccgcgca cgacttccag catacgttgc
    14821 cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca ccagcgcacc agcattaata
    14881 aacggattac gcggaatacc ctgctccatt tccagttgca ctaaagaatt gaacggtgat
    14941 ccagacggat ctttgccgac gcgttgccag atttcctctt cggagtaatg acgcatggcg
    15001 acaacgagac tcagcacttt ggaaatagac tgaatggaaa aacgttcttg cgcgtctccg
    15061 gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc ccaatcggga accgtctact
    15121 gtagccagcg ccggaatata atccgcgact ttaccctgac caatgagcgg ccgcacttgc
    15181 cgcaagatgt tttctaaaat tgcattatcc atggcgactg ccactttcta ctcctggacc
    15241 gcaggtctga aaagacctgc gagtatatca gagctgaata tgtcgcgtca gatccggtct
    15301 ttccacaccg tctggatatt acagaattcg tgtaagccga aatgggaaag ctcacgacca
    15361 aagccactct ttttcacgcc accaaaggcc actcgcgcgt cgctggcaca ataaccattg
    15421 ataaacaccc caccgcattc cagacgtgcc gccatctgtc tggcctgtgt ttcgtcggtg
    15481 gtaaaaatgg tcgctgaaag gccgaactca ctatcattag ccagttccag cgcatgttct
    15541 gcatctttcg caatggtgat tgccgcaacg gggccaaaca tttcttcccg aaaagcggtc
    15601 atttctgggg taacattcgc cagaaccgtt ggcggatagt agttacctgc cccagccatc
    15661 ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg ttttctccac ctgatgatgc
    15721 agctcatcac gtaaatcgaa acgagccatt ggtccgagag cgttctcttc gtcacgggga
    15781 tcgtccattt tcaaggctgc cgcagctgcc acaaaacgtt cggtaaatgc cgaagcaatt
    15841 ccctcttcga taataaagcg ttttgctgct gcacaaacct gtccggtatt ctgataacgt
    15901 ccggctaccg ccgctttaac tgccagttcc agatcggcat cgttaagcac aataaatgga
    15961 tccgaaccgc ccagttccag tacgcatttt ttcagtgccg ctccagcctg tgcaccaata
    16021 gccgctccag cacgaacact tccggtcacc gtgatagcag caatgcgcga gtctttaatc
    16081 atctgagtga caccgtcatt gtcggcattc agccagccat atacgccttg tgggattccc
    16141 gcatctttaa acacctgggc aatgagctga gcacagccca tcacattcgg cgcatgttta
    16201 agtaagtatc cgttaccagc aagaatgatg ggaacagcgc cacgcatcac ctgccataac
    16261 ggaaaattcc acggcataat cgccagaatc gtccccaacg gtcgatactc aataactgcc
    16321 tgctgatttt ccaccagcgt aggttccgcc ttcagcattg ccggaccatg ttctgcatac
    16381 cagtcacaca aattcgccga tttcgccact tcagcgcgcg cctggttgat tggtttgccc
    16441 atttcgcggg tgatcatttg cgccatttct tcgctacggg cgcgcagagc cttaccgata
    16501 tcacgcagtt tttcagcacg ataatctata tttgtctcgc gccagtcacg aaagcctgct
    16561 gccgccagct gaagtgcgtt ttcgatatca tcagcgccag cccacggcaa cacagaaagt
    16621 tgttcacccg tggcaggatt tatcgaaatt gcatgagttg ccggagtaat ggtcatcggg
    16681 gtatctcctt tatgagtcat ggtatgaaga tacgcagatt tactcttgct ttaaaatgaa
    16741 taatattaag ccacttattc acgaatcgag aatattatgg atctgaccca actggagatg
    16801 ttcaacgcgg ttgccgaagc tggcagcatt acgcaggctg cagcaatagt gcatcgcgtg
    16861 ccgtcgaatc tcactacccg tctacgccag ctggaaacag aactgggggt agatctgttt
    16921 attcgcgaga atcagcgttt acgtctatct cctgccgggc ataacttttt acgctatagc
    16981 caacaaattc tcgcgttagt ggatgaagcg cggagcgttg tcgctggcga tgagccgcaa
    17041 ggtttatttt ctctgggttc gctggaaagc accgcagcag ttcgcattcc ggccacgctg
    17101 gcggaattca accgtcgtta tcccaaaatt cagttttcac tttccaccgg cccttccggc
    17161 actatgctgg aaggtgtgct ggagggaaaa cttaatgcgg cgtttattga tggacccatt
    17221 aaccatactg ccattgacgg gataccggta taccgcgagg agctgatgat cgtcacgcca
    17281 caaggtcatg cgccagtaat ccgtgccagt caggttaatg gcagtaacat ttatgccttc
    17341 cgcgccaatt gttcgtatcg tcgccacttc gagagctggt ttcatgctga cggtgccgct
    17401 ccgggaacta tccatgagat ggagtcttat cacggaatgt tggcctgtgt ggtcgcagga
    17461 gcaggtattg cgcttattcc gcgctctatg ctggaaagta tgccggggca tcaccaggtt
    17521 gaagcgtggc cgttagctga gcaatggtgt tggctaacaa cctggctggt ctggcgtcgt
    17581 ggtgcgaaaa cgcgtccgct cgaggcattt attcaactac tggatgtgcc tgactcgaca
    17641 aaacagggtt accaatgagc tatttttgat agttcttgcg gctaatatgc tctatatagt
    17701 gatgttccga tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca
    17761 attacatatc tcttcctgat gattttcccg ttcgtaatat tgcaccg
//
LOCUS       DBFIFS010000079        17745 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.079, whole genome shotgun sequence.
ACCESSION   DBFIFS010000079 DBFIFS010000000
VERSION     DBFIFS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.079"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            137..784
                     /locus_tag="N7A07_002393"
     CDS             137..784
                     /locus_tag="N7A07_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU2477047.1"
                     /translation="MILKINQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAV
                     GAYNAGFRKSERQNQRRLAYASEVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            complement(1081..1902)
                     /locus_tag="N7A07_002394"
     CDS             complement(1081..1902)
                     /locus_tag="N7A07_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2477048.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(2008..2304)
                     /locus_tag="N7A07_002395"
     CDS             complement(2008..2304)
                     /locus_tag="N7A07_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477049.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLMKQLGQINDELGALLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTSPV"
     gene            complement(2336..2458)
                     /locus_tag="N7A07_002396"
                     /pseudo
     CDS             complement(2336..2458)
                     /locus_tag="N7A07_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2528..2824
                     /locus_tag="N7A07_002397"
                     /pseudo
     CDS             2528..2824
                     /locus_tag="N7A07_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3125..3247)
                     /locus_tag="N7A07_002398"
     CDS             complement(3125..3247)
                     /locus_tag="N7A07_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2477050.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESGGK"
     gene            complement(3192..3344)
                     /locus_tag="N7A07_002399"
                     /pseudo
     CDS             complement(3192..3344)
                     /locus_tag="N7A07_002399"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3494..4280)
                     /locus_tag="N7A07_002400"
                     /pseudo
     CDS             complement(3494..4280)
                     /locus_tag="N7A07_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4277..4711)
                     /gene="psiB"
                     /locus_tag="N7A07_002401"
     CDS             complement(4277..4711)
                     /gene="psiB"
                     /locus_tag="N7A07_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU2477051.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDGQGYSVNDIIHILMAEGGQA"
     gene            complement(4766..6724)
                     /locus_tag="N7A07_002402"
     CDS             complement(4766..6724)
                     /locus_tag="N7A07_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU2477052.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDAPENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDTTERTDNLADAA"
     gene            complement(6788..7021)
                     /locus_tag="N7A07_002403"
     CDS             complement(6788..7021)
                     /locus_tag="N7A07_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HGU2477053.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(7077..7607)
                     /locus_tag="N7A07_002404"
     CDS             complement(7077..7607)
                     /locus_tag="N7A07_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2477054.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNNGVTRYITEI
                     LVKNTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEP
                     QQPSEPAYDFDDDIPF"
     gene            complement(7633..7839)
                     /locus_tag="N7A07_002405"
     CDS             complement(7633..7839)
                     /locus_tag="N7A07_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2477055.1"
                     /translation="MHIPQKFLPKSRQKLLWSAPGQSFPAVAVMPLAAKVLKRVMVNT
                     GGNKRQVPAKRQNRGSRNSTGDGG"
     gene            complement(8131..8442)
                     /locus_tag="N7A07_002406"
     CDS             complement(8131..8442)
                     /locus_tag="N7A07_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477056.1"
                     /translation="MKTPGRREDKCLSVRAGKRPRAGGAERGRADAPPLSLRGRSDGG
                     FCLFRPVICLLLMMLLFLLLLLLLLLLLFFFFLMLSLLSVLLFSCLCLFRASPWRGGL
                     S"
     gene            complement(8439..9002)
                     /locus_tag="N7A07_002407"
     CDS             complement(8439..9002)
                     /locus_tag="N7A07_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2477057.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTRVPTMIIRLRA"
     gene            complement(9050..10411)
                     /locus_tag="N7A07_002408"
     CDS             complement(9050..10411)
                     /locus_tag="N7A07_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGU2477058.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSARLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKFISSYDRISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERTMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMGNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10463..10693)
                     /locus_tag="N7A07_002409"
     CDS             complement(10463..10693)
                     /locus_tag="N7A07_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477059.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            11210..11352
                     /locus_tag="N7A07_002410"
                     /pseudo
     CDS             11210..11352
                     /locus_tag="N7A07_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11308..11534)
                     /locus_tag="N7A07_002411"
                     /pseudo
     CDS             complement(11308..11534)
                     /locus_tag="N7A07_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426396.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11722..11913)
                     /locus_tag="N7A07_002412"
     CDS             complement(11722..11913)
                     /locus_tag="N7A07_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477060.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTVQIVDEICDFVV
                     NQQAVFLGGHYIFQGSRHR"
     gene            complement(11910..12332)
                     /locus_tag="N7A07_002413"
     CDS             complement(11910..12332)
                     /locus_tag="N7A07_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2477061.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12379..12804)
                     /locus_tag="N7A07_002414"
     CDS             complement(12379..12804)
                     /locus_tag="N7A07_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2477062.1"
                     /translation="MQYAKSVILNVEECDRLSFLPYLFGQDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(13217..13993)
                     /locus_tag="N7A07_002415"
     CDS             complement(13217..13993)
                     /locus_tag="N7A07_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477063.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASVIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDVVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRG"
     gene            complement(14044..14511)
                     /locus_tag="N7A07_002416"
     CDS             complement(14044..14511)
                     /locus_tag="N7A07_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2477064.1"
                     /translation="MRALKTEKETRMYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMEISLTDHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            complement(14492..14713)
                     /locus_tag="N7A07_002417"
     CDS             complement(14492..14713)
                     /locus_tag="N7A07_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477065.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            complement(14714..15397)
                     /locus_tag="N7A07_002418"
     CDS             complement(14714..15397)
                     /locus_tag="N7A07_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU2477066.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRQGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRIDRFMAAWKRAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFEPEAA"
     gene            complement(15473..15784)
                     /locus_tag="N7A07_002419"
     CDS             complement(15473..15784)
                     /locus_tag="N7A07_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477067.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(15781..16683)
                     /locus_tag="N7A07_002420"
     CDS             complement(15781..16683)
                     /locus_tag="N7A07_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU2477068.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGVTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            17122..>17745
                     /gene="parM"
                     /locus_tag="N7A07_002421"
     CDS             17122..>17745
                     /gene="parM"
                     /locus_tag="N7A07_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HGU2477069.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHT"
BASE COUNT         3989 a         5186 c         4670 g         3900 t
ORIGIN      
        1 atataaaata atgtttttta taaaaatagt cagtaccacc cctacaaagc ggtgtcggcg
       61 cgttgctgta gctgcgttaa cgacgctgct ttaaataaat cagatttaaa caatataaat
      121 ccacaaatac aactcaatga tattaaagat aaatcagcaa aaacttgttt ttgcgtgggg
      181 tgtggtgctt ttggtggtga gaaccaccaa cctgttgagc ctttttgtgg agtgggttaa
      241 attatttacg gataaagtca ccagaggtgg aaaaatgaaa aaatggatgt tagccatctg
      301 cctgatgttt ataaatgaga tctgccatgc cactgattgc tttgatcttg caggccggga
      361 ttacaaaata gatcctgatt tactgagagc aatatcatgg aaagaatccc gttaccgggt
      421 taatgccatc ggtattaatc cggtaacggg atatggcagc ggactgatgc aggtagattc
      481 ccagcatttt aacgaactgg cccgctatgg aattaagccg gaacatctga caacagatcc
      541 ctgcatgaac atttataccg gtgcttatta tctggcaata gcatttaaaa aatggggcgt
      601 cacctgggag gccgttggtg catacaatgc aggattcagg aaatccgaac gccagaacca
      661 gagacgtctt gcctacgcat cagaggttta ccggatttat accgggataa aaagcagtaa
      721 aggtatccgg cttccggcca cgaagaaatc acttccggaa attaacagcg tgcagaacaa
      781 ttaatcttac agacattgtc agataccggt tatgccgcga tagcggcatg gaaggccaca
      841 cacaaaaata tcatatgagt gacaatactg actggccgaa gccccgcgaa gcgggaaccc
      901 atcttatata tacccggtaa ctgcgcagcc tcctgccaga ccatttttcg cgggcacaat
      961 atttacagac aaccggaaag ccggtcgatg aaattgaggt atatcagacg acacagcaat
     1021 gccgaaaaat gataatataa gcaaagcccc gaaaattttt tcggggcttt gcttatatta
     1081 tcatgacttc cactttctga actgttcggc aatcacccat aacgccttgt tgattcttat
     1141 atcaccatca atacctgtaa tggctcttgt cctggtattt ttcccggagg cacttcgccc
     1201 cgataatcca ccttttatca tattctcctg aacccgctgc caggttgtcc agagatcgtt
     1261 ctgtttgtct tcccgacgac ggggagtaat tatctgttca ggtgtcactg gcgttttatt
     1321 ttcatcttca tacctgacca tcagtgcagc tctgccaaat aaatgttgct cgtcactgtt
     1381 aagatgaatt tctttcatcg cctccatatt atcagtgacc ttatcaaaga caccgagcac
     1441 ttcatacgct ccctcgataa cctgcccgac aatatcacct ttatgtggaa cgcggatttc
     1501 gccaaaatta tttccgcaca ccaggccatt tgtgcagaca aaacgaaaaa ttccggggat
     1561 catctgataa ctggatgaac catcatgtga attaagcagg ataatttcag gaacttcctg
     1621 tccgttaata tgcccttccc tgcgaagacg taacatatgt ttactgtatt cgcgacgtcc
     1681 caaatcacga acccgactct gacaggcaaa gaatggctgg aaaccttcat cacgtaactt
     1741 attgatgata ttgattgttg gaatatacgt ataacgttca ctccgggact catgtttgtc
     1801 accagaaaaa acagacggta caaactgcat taattcgtca tccgttaacg ggcgctcacg
     1861 gcgaatggaa ttatatcggc caaaacggga agccagtcgc ataatattca ctcctcgctg
     1921 aaagaaaaaa taagtcaaaa attttttcgc ttaatttcct ccccctgatt attattaaca
     1981 aatcgacaac aatcagggaa aaaatcatca caccggactg gtcatgggct gaccggcctg
     2041 ttggctgaaa agcatcatca gtgcactcaa atcactggct ttttttctga taactggcgt
     2101 gtgattttcc acgctgtcca acagcgcccc cagttcatca ttgatttgcc ctaactgttt
     2161 catcagcgtt tccgcatcca ggctgctctg gtatgcaaga tttgcctgaa cagcacgatt
     2221 ctgcatgatg tcgatttgtg cgattgcatc cttctgctga tcgccggttg gctgataagt
     2281 ggtgttaaaa gaaaatgcgc tcataattcc tcctgtatct gatgttttca gcccccatca
     2341 cccgtttgag aaccttcgcc gcaaggggca tcactgccac gaccggaaac gactgtccgg
     2401 gggcagaccg caaaagtttt tgcctgcttt tgggcaaaaa gttttgcggg atgtacattt
     2461 cacccctgga ctggcggaga cggttgtggc tgtctgtgcc cccggcgaca gggactcata
     2521 cgggtgtgtg gcaccggaca caacaacggg cagggacccg ccgcaacgac gaagagcgcg
     2581 tgcgcgaaac ggcgttgccc cttacatccc ggcggccttt tgccgggatg ccgggagcgc
     2641 aggccacggc gcgacagagg cggcgcgacc ggaacggtcg ctgcaagcca gggggttcgc
     2701 gtcagggatg gaagcccgcc agggcggaga cagctccgct ggctccgttc acgacagccc
     2761 gccccgccag ggggacgccc ggaaaaggca taagcaggag aacaacaaaa cagacaataa
     2821 tcagggcagc agtaatcaga gtcagaacag gaggaaaaag agaataatca aagggaaggt
     2881 gatgtgattt ctgacaggtc ataaacagag aaggcggggg aaaagaacgg gcgccgcagg
     2941 cgcacgttca tccccgttcc cggagggaaa cggccttcct ggcagtctgg ttgttcatgc
     3001 ccgcacaatg ccagcaagga gaaaggggcc accggcgaac cagcagcccc tttataaagg
     3061 cgcttcagtg cagtcagacc agcatcagcc ctgaaaaggc gggcctgcgc ccgcctccag
     3121 gttgctactt accgccggat tcgtaagcca aacttgccgt aacctccctg tacccgtcct
     3181 tcagccggac ttcgcacaga cgattccggg tcaggagtgt gaatattaac aacgtgcagc
     3241 acacgattaa tacgcaccag ataagggcgt ttcccggtag tttcatggtt tactcctcct
     3301 tgcaatttgc acggtaagag gccatactga cgtggactag acatgcagag gcctcgtggg
     3361 ttaatgaaaa attaactacg gggcttttgt ccttctgcca cacgacagga taacaaacca
     3421 ccttcacgtc atgaggcaga aagcctcaag cgccgggcac atcatagccc atatacctgc
     3481 acgctgacca cactcacttt ccctgaaaat aatctggtcg ttcagccagt tcacgggaaa
     3541 tccgtgtgat tgttgccgca tcacgctgcc tcccggagtt tgttcggcac catccatcgc
     3601 tccatctcac ggacggcatg accggcctct ctggctgcca gactggcttc cgcgatgacc
     3661 tgccagaatg gcatatcctg ccactgccag cgttccattc cggccagtga cggaaaacgc
     3721 tcaccccact gccagaacag cgtttccagg tcatgctccg ccacccggtc actccagcgg
     3781 gcatgccagc ttccgaccgt ctccggcgaa tggaatgcca gttcgatttc agcctgagca
     3841 accagcgcct ggtacgcacg ggctttctgc tctgcctccc gtgcctgctg gcggctgtac
     3901 ttctgcatga ccagcgcgga tttatggcga ctgcgttccc tgacacggaa ccgcaccgac
     3961 gggaacagcc gctctgctgc atcaccggga aggggaagga ggcagacctc gccctgtgtt
     4021 tcaatcagca catcgatggc atacagccat tgaagacggt tgccgtgcag ttcttctgcg
     4081 gtcagggacg gcatgaaaaa tcgcaggtca gacagcgata tcctgcggct tccgttcaga
     4141 cagcggaaaa acgcccccgc atacggatat tccgccagcg agtttccctg atggcggcgt
     4201 ttttccgtct ccgccaccgc ccgccatgcg gcctgttgct ctgcgctgag gggaaccagt
     4261 gcctgtgaac gtgcgctcat gcctgacctc cttccgccat caggatatgg atgatgtcgt
     4321 taacgctgta cccctgccca tccagtgacg ccgccagtgc cagtgaatgg ctgacagctt
     4381 ccggtacaaa ccgccgctgc acctgaacca ccgcaaacgg ctcaccaccg gcattcacca
     4441 gaaccagcac ccagacctga gagacatcgc ccggtgaaca taacgccagg tgaaaacgtt
     4501 cgtgggcagg cgtgaagcgg acctggacgg ggaaaaagcc gccgaactcg ctgccgtact
     4561 cgccattcat cgtccagttg tccggcgcat ccagttcccg catcacggcg tgtgtcagct
     4621 cacggcgttc atcacttccg gcagcccgga tatcttcata ttcctgtgca ctcatggtct
     4681 gtaatacatt cagggtcagt tcagttttca tcacggtatc tcccttgctg ccgccccgtc
     4741 tccggcgggg cggtgtggtt gttcatcagg cggcatcagc caggttatcg gtgcgttcag
     4801 tggtatccgt ctgtggacgt ggtgcacaca tccagccagg cacccagcgg ttgtctttca
     4861 tatggtgctc tgcatgttca gccgcatcac ctttcttcat cttctctgca tcccgtgcgg
     4921 caccggacag tccggcatca ttcagggctg cgataatctg cggctttttc aggtgtccga
     4981 agaagtttgc ttttgtcggc tgccaccagt ctcgcatgtg aaagccgatg gcggtttcca
     5041 gcgtgtcaag cggactgcgt gacgtgtgac cacactcacg ggtctggaca ccgttaaggc
     5101 tgcatgcggt gcagaaactg agcagggata acagcacctc ctgactgagt gacaggaatg
     5161 tcgtcatgtc ccgtgaccat ccctccggta acagggcagc aagacgggct ttttctgcca
     5221 tcagcgccat gaatgcggca ccttccttcc ccgatggcgc atcgctggtc agcgaataat
     5281 gtttacattc caggctgatt tgtgctggtt tactgtacgc tccgctgcca aacacattca
     5341 ggcagagcgt ccatgccagc agtgccagag atttgtccgg ttgctgcatc agtgctgcct
     5401 ggactgccag cgtgcgttcg gaagacattt tcgtcagcag tggcagactg atttcctcca
     5461 ctgacgcttt ctcctgcacc tgttccgtac ggtcagcgtc gtcagccacg tcatcctcac
     5521 tgcgcaactg cacaccacgc tggacacaca cgttgccata acgccaggac accaccactc
     5581 cacctccggc acgcatctcc ggcgtccacg ccctgacctt cgccatgcag cgcatcagct
     5641 tcatttctgc ttccagcagg tcagattcct cacactggtt ttccggcgcg tcgtaacgcg
     5701 tcatcagttc gttcagacgt tcttcttccg cctccgtcag caccgcttcc ggctccggca
     5761 gacagcggta tgttccggca tcctcacggc actcaccgac aggctccatg cgtccggcgc
     5821 accattccca gccttcggct tcccgaagga actctgcgac agcctggagt ttttccagca
     5881 gggcggcatc gagcgccacg cagtccacat agccaccttc gtcgtcgctg aacaaatcgg
     5941 tgcgcagttc gtctggcgag aaggcatcag cccccacgaa gcggaattta ctgttccccg
     6001 ccaccgccac ttcactttcg gtcaccagac gacgaatggt ctgtacttcc ggtttaccgc
     6061 cccatcccga ctgacaggcg gcttcaaaca cctgcacctg acgcgcggtg tcgttctcca
     6121 gcgccagcgc ctgacagtgt tcggtggtga tgcggtcttc tgccagcgca tcgaggatga
     6181 caggcgcaag gtcagccagt ttcagcattc gctgaacgtg gcggggtgaa tagcccagca
     6241 aatcaccgat ttgtgcaggt gttttgcctt cctgcgccat tgcgcggaat ccggcaatct
     6301 gttcggcagg gtgcatatcc cgacgctgac cgttttcagt cattgatgca gccgtcgcca
     6361 gctcctgcgg aataaccttc acgcgtacag gccagtcagc cggaaggatg ccacgctctg
     6421 ccagcatgtt gagtgctgcc agtcggcgac cacctgcggc gacaccgtgg cggtcaccag
     6481 gcagggcatg aacaaccaga ttctgcaaca ggccaacgcc cttaatggac tccgccagtt
     6541 cgctgacgga ctctgcggaa tacggcaccg tgcgcacatt cagcggtgat ttaatcagcg
     6601 aggccagcga cacgctcact tcctccgtct gctccagcag ggcagacagg actgtttcct
     6661 gcgtttttgc aggtttacgg ctggatttac gctccgtttt tgtcttagac tctgtaactg
     6721 acatatattt ctcctttgac cgggatggtt gtatgtctgc cggacgcgtt cccgtccggc
     6781 acctctctta cttaagaatg ccgaaattcc ggatgacatg gttcatccag tacccggcac
     6841 cgtcctcaaa attccaggta cgccagacca ggccactgtt attgcggaca accaggcgaa
     6901 aatgcgttcc ctgatcatcc tcgataaaga cattccggta ctgtgcggca acggcttcag
     6961 cctgttcgcg ggtaaaggag ccgtcaggca gcccctgaaa tattctgaaa tattcgctca
     7021 tcctcatatc tccggtgatg ccccgcactg gacggggcgg ttgtcactgt ctttcgttag
     7081 aacggaatgt cgtcatcgaa gtcatacgcg ggttcagacg gctgctgagg ctccggctgc
     7141 gcggccttac gtccacggcc tttcgttttc gcgccacctt ttttcggttc cggctgtgat
     7201 tccggctgag gctgaccgct gaactgttgc gcctcttccg gctgagtctg agtacctgcg
     7261 gcacgtccca gcatctgcat ggtgcccgtg tttttaacaa ggatttcagt gatgtaacgg
     7321 gtgacgccgt tgttgtcgtc ccagctacgg gtacggagtt gcccctcgat gtagacctgc
     7381 gcacccttac gcaaatattc accagccact tccgcgagct tgccgaacaa caccacgcga
     7441 tgccattccg tctgctcccg catctccccc gtctgtttgt cacgccagct ttctgacgtg
     7501 gccacctgca ggtttgccac tgcgcccccg ttggggatgt aacggacttc cggatctttt
     7561 cccaggcgac cgacaagaat gaccttgtta attccacgaa ctgccatgat gtgtctcctt
     7621 ctgttgatgt tttcagcccc catcacccgt tgagtttcgg gagccgcggt tttgacgttt
     7681 tgcggggacc tgccgtttgt tccctccggt attaaccatc acccgtttga gaaccttcgc
     7741 cgcaaggggc atcactgcca cggccggaaa cgactgtccg ggggcagacc acaaaagttt
     7801 ttgccggctt ttgggcaaaa acttttgtgg gatgtgcatt tcacccctgg actggcggag
     7861 acggttgtgg ctgtctttgc ccccggcgac aggggcttaa acgggtgcag ggcaccggat
     7921 acaacaacgg gcaggcaccc gccgcaacga cgaagagcgc gtgcgcgaaa cggcgttgcc
     7981 cctcacatcc cggcggcctt ttgccgggat gccgggagcg caggtcacgg cgcgacagag
     8041 gcggcgcgac cggaacggtc gctgcaagcc agggggtttg gcgtcaggga tggaagcccg
     8101 ccagggcgga gacagcttcg ctggctccgt tcacgacagc ccgccccgcc agggggacgc
     8161 ccggaaaagg cataagcagg agaacaacaa aacagacagt aatgagagca tcagaaaaaa
     8221 gaagaacagt agcagtagca gtagcagtag cagtagcagg aacaacaaca tcatcagtaa
     8281 caggcaaatg acaggtctga aaagacagaa gcccccgtca gaacgtccgc gtaatgaaag
     8341 gggcggcgcg tcagcgcggc ccctttcagc gccaccggcg cgcggtcttt tcccggcgcg
     8401 gacagacagg catttatcct cccgcctgcc cggcgtcttc aggcccgcag acgaataatc
     8461 atcgtcggaa cacgggtata agcaaacgtg ccgcgcggca gctcctcgcg gacgtcagaa
     8521 aacggcaaca gcttctcctg ctggcgtggt ccgttgagac agacggcgac cagcacgcca
     8581 cccggacgca acagggaaaa ggcccgcagg atatgccgga tatcctgccc gtggctgaac
     8641 ggcggattca tgataacccg gctgtaatac tgcaccggct gccattccat gaagtcgcca
     8701 cactgaaccc tgacaccgtt aaaattttcc cgcagatacc ggaccagccc gctgttgatt
     8761 tccaccgcat cacacatggc ccccggcgcg gtatcccgaa tcgcccgcag tatggccccg
     8821 gtgccggcag agggttccag aatgtgatca cggttgctga tctccgccag cgtcaccagg
     8881 cgatcacaga ccggggcagg agtcacaaaa agctgagatt cagcccggat ttcagcaacc
     8941 ggcgcggcgt tatccagcct tgcgcgcatc agccggatac gttctcttaa ctgactgtgc
     9001 atatcattat ctccgtcatc ccccggggat tttcccgggg ggatatacct tatttctgtg
     9061 ggatttccgt gattttcatg tcggtgatat acacattcac caggcggaaa ttattgccca
     9121 ttgtgcggcg gtaccggtat gccccgtggt cttctgcttc tgccacactg cgcacggcct
     9181 tacagtcccg gggcagggcg gcccactgtg cctttgtcat ttcccggaaa ccttctcccg
     9241 gatagttgac taccggcgga cgcttcgcgg cctggctggc gacggcagcc tcttctgccg
     9301 atggtgcttt gtagtccgtg atgcgatcgg gcgtcacttt catcgtgccg ctgtacccga
     9361 ggaaactgta attcggcgtt gtgactgaac tcaccgcccc gttgcttttg ttcacacgga
     9421 tgatggtcag ccactcgccc cggctgaaaa cctgtccgcc cggctcaaaa tcctgtgtcc
     9481 gggtaaccac gccgccgctt tcgtccagca tggtacgctc atagatcagg cggttctgat
     9541 agtggttaac ccagcgttgt tgatgctgaa tctgccgttc atgacagcgg atcgcaattt
     9601 cgcgggcctg ctccgtggtg atgatgtcat catccagggc cgaccataac gaccggctcc
     9661 cttcatactg gcttttttct gccgggcgcg gatacgtatc cagcgggaaa caggcactga
     9721 tacggtcata actgctgatg aattttgcca tattcagatc cagcgattca gcccgccaca
     9781 tcgtcagata tttctgcgac tgtgcgatgg tcttttcagc cttgcgcaaa tcagcttcga
     9841 tttttttgat gcgacgccag cgaacgtccg gacgttcttt atacttcgcg tgaagccgtg
     9901 ccgaccgcgc ccgctcttcc cagtattccg cacgttcaaa gagcatcacg gcacgtttca
     9961 tgccgttttc aatacgctgt gcatcacgac gggcgcggcg ttcgctgtga tgccccacaa
    10021 gaatgggctg accgggcggg atcatcgcgg ccagtctttc cacttcatca agtgcctgtg
    10081 cggattcact ggcccgcttt ccgctgtatc cggtaaaccg ctccgcccgt gcttcctgac
    10141 gttcagcgag cgtgctgtct tcatcctcaa tctctccggc aagtgagagg agcacgtctt
    10201 cccggcccgg tgtccaggct ggcgcgacaa acagcgcctg tttgggtgcc cagcgaaaac
    10261 cagcatcatg cactttttta tacgtctccg ggtcaagacg tgacacggca tacaggcgca
    10321 gtttgttgtc atccggtgag taggtcgcgc ggtacatctg cacagggctg gcggcttccg
    10381 gggcggtcat ggtgattgct gctaatgtca taaaatgttc tccttccgtc tgttgccgcc
    10441 cctcgcgggg cggctcacct catcagttaa aagcgcgttt cagttcgagt gtgcggagca
    10501 cctgcccgaa agctgtgttt ttcatggcgg ggatgtgtac accacgcccc cggcgaaaac
    10561 tccgggctat gtgtcgcccg aaggctttca gcacggggtc aatttccggc gcggggatgt
    10621 cttcgcgtct gaaacgggga tgacggcggg gctttacagc cgctgcggaa ggtgaaacaa
    10681 tacttaccgt cataaatgaa actccttatg actgttgaat ggaaccgcag aaactgctcg
    10741 aacagttttt gcggtgtgga ttaagcaggt cctccccggc ctgctttttt catatctgcg
    10801 tctgacgttc tccggtgttt tcctccttct gttcctgcgt tctcattacc tgccagccgg
    10861 atgactgaca gaacacataa atgcgtattg ccggagcctg aaataaatcc tgatttttaa
    10921 agaacatcac ccgttgagtt tcgggagccg cggttttgac gtttcgcggg aacctgctgc
    10981 tgtattccct gtggtatgaa cttcacccgt ttgagaacct tcgccgcaag gggcatcact
    11041 gccacggcct gacgcacctg tcctgggcag aacgcaaaat tttttaccgg gttttgggta
    11101 aaaaattttg tgggctgttc atttaccctg gacagtcagt gcggcaggtt gtggctgtat
    11161 cagcccccgg cgacagggac tcatacgggt gtgtggcacc cggcaggcaa tgagcaggga
    11221 cccgccgcaa cgacggagag cgcgtgcgcg aaacggcgtt gcccctcaca tcccggcggc
    11281 cttttgccgg gatgccggga gcgcaggtca cggcgcgaca gaggcggcgc gaccggaacg
    11341 gtcgctgcaa gcctgtggtt ttgacgcatc ggaccgtagc gacagcccga aaccgcgcca
    11401 gcgggttcgg cggagactgg cgggtgcgcc agcagctgcc tgtcggagtc gggcctgacg
    11461 accggatgcc gccaggcagc cgggagccga aggtgcgcag gcagacagga caacactgca
    11521 gtagtcagaa tcagggcagc agagaaaaga acaacaacaa caggaacagg gtcataccat
    11581 cagaaaaaaa cattccggac acatcacaaa cgggaagggt aacgggcgac gcgccagcgt
    11641 ggcccgcttc ccttccggca ggcgtcagcc tgccagggtt ttcgcgcggg ctgacaggcg
    11701 gcttccaccg cctgccctgt atcacctgtg tctgctgccc tgaaagatgt agtgaccgcc
    11761 gaggaaaacc gcctgctgat tcaccacaaa atcgcagatt tcatcaacaa tctgcacagt
    11821 ggtgagcggt ttctgcccca tttcacgacg ccgtgcatta atcacggctt tgtaattgcg
    11881 cagaatttcg cgggtttctg tgctgatatt catgacatgt gctccccgtc cagcgcctgt
    11941 aacacaacgt ccattgcttc gcgttcttcc ctgacgaatg cgtcaccgtc gcccccgttc
    12001 tcactcccga cggcatacag gcgcttcatt tcactgcctg ccagcgccat cactttttcc
    12061 agcatcaccg ccgggactgt cacctgacgg ctggcgcgct tttcttccat cagttcacga
    12121 acaacgtcgt gaacaacgct gacatccgca ccatgctcaa aggcatcgtc cagccgctgc
    12181 atgacggttt ccagctcatc gtctgacagg ttccagtcct gggctatgtg acgcacatcc
    12241 ccacgggtca gcaccacggc aaaaacgcat tcactgtccg gcacgtctgt atccagcact
    12301 tcgcggataa tttctttaac agtacagtac atgttctcgt ctcctttaca ggggcggcat
    12361 tgccgccccg tccgttaatc agtccagagc cgcgtaaatc gcgttgcatt cagggtgaaa
    12421 ttcgatgtga cgccacaact gcgcatcgcg catccggtaa agctgggtca gtcctgcatc
    12481 accgctgtcg tggtacagcc acaactggcg gttaatcaca agggaagtca gaatgatgcc
    12541 gcaggcatca gcactcacgg tacggtcaaa ccagtttgca ccgttcacca tgtggaaacg
    12601 gtcgccatcc ggcatcatgt aaccgccacc gtccggcagg cggatgaagt gccagaatcc
    12661 gccttcatat tccggcatca tttttttcgc cagcgcgtat acagacgctt cggcatacag
    12721 aaaatcctgg ccaaacaggt aaggcagaaa agacagacgg tcgcactctt caacgttcag
    12781 aatgacagat tttgcatact gcatgggatt tcctccgttg ttcacatatt ccgcgacacc
    12841 gggcggtgcc gtgcctccat aaaagccggg cgataaggcg gggatgcaca gggcgcaagg
    12901 gaagccgcag cctgacgcac acggctggcg ggagttggag ggtgagaccg cgaaacctga
    12961 gcgggcgcac gaccggaaaa tcacggcaga cgggaaggag ggaccgggtg accgcagcgg
    13021 cgaagcgtac ccttgcatca acgccagccc ggctaagatg gaggcacggc acagcccgca
    13081 cacgacgctg aggccacata actgaaacac cacaaggcca tgccctgcat ggccgggtag
    13141 agcggagccg gcggcgacct ccttttgctc tttccggaaa ccggcaggaa aaaagcgggg
    13201 attttccccg ccatcatcag cccctgattt ttgccagggc atccgccatg ctgcggattt
    13261 cgttccgggc ttcccggacc acctcccgca gaacaccacg gtcaaaccac cggcggggcg
    13321 gttcatccgg tatacgcagc acatcgcagg ccagcccact ggacaggata tgttcaatac
    13381 aatcgctgac cagatacatt tccaccgccg aaaaacgtgc gccccggttc aggatggcat
    13441 cgagcaccac gggcaggttt tcgtcgtcct catcgagcca gcaggggtga tcgctttcct
    13501 tcacgaccag gcgaaagcgg cgcgtctggc gggtcagtaa cgtctgaccg gtggtggtca
    13561 gcgccgcccg gatacgccgc gtatcttctg ccacccggca actgacactg cgtaccacat
    13621 cgcgcaccgc cgcaagtaca gccctgattt cgtcttcggt cagccattca ccggcatacg
    13681 gaaaagacac ctgcgttccg tctgccggaa gcaccggcag cgactgccac tgactgccgg
    13741 acagaacaag ttccgctccg gttccggtca gtttgtgaat gacggaggcc aggagcggtg
    13801 gcagtacgcc gccgtgacgg ttgataagta acgtcgccag tcggggcgca tccagcagaa
    13861 cataaatgct gatccagccc ggatacttcg ggttccgctg gtcaacatcc gtctcttccg
    13921 gccagccctg cgccagcagc aggttacggg cgtgacgaca aatcagtgcg ttcagcgtac
    13981 tgttttcgtt catgattaag cgttctccct gtatcgccgc ccgcagggca ggcggcgata
    14041 acatcagttg ttttcgtgtg tgttgttttt cagcagggtg cgggcctgat taaccaccgc
    14101 ctgacggcgg gtaacgcatt ccgggacggc gaggccgtta tcacgggcgg cccactcacg
    14161 tttccacagt gcctgttcag cggtctgaat cagggacgcc agcagttcag cagggacggt
    14221 cacctggcgg ttttcgctca cgttgctgat aatctccatc accgcggcgg aagaaatacc
    14281 ggattcagtc cgctggtctt ccgggatgtc ctccatgcgc gccaggacgg tcctgatttc
    14341 atgatcggtc agagaaattt ccataccgtc agccagtgcc tgaatctctt ccggagacca
    14401 gataaccagc atcagtggcg tgtctcccgg atactttgct gccagctccc ggcacagcgt
    14461 ttcgcatgtt ccgtacattc tcgtctcctt ctcagtcttt aaagcacgca tccagttcca
    14521 gaatttcggt atatgcctcc atcaggaggc ggtttgcgcg aaacagcgtc tgacgcacca
    14581 gacgttccgg cagggtgtcg gcattaaagc tgtaccgctg ctccatctca ttcatggttg
    14641 tacgcaggtc gcacatggca ttggccacct ccgccacttc ggcgcgaagt ttttcgtgtc
    14701 cggcatagtt catttacgca gcctccggtt caaaccagtc atcattcgcg gccccctgct
    14761 gcatggcccg ttgcacggcg gccagtcgtt gctgtccggc acggtgatac tgttcaagca
    14821 gctcgatacc gatataccgg cgtccggact gaagggcggc gacgcaggtt gagccgctgc
    14881 ctgcaaacgg gtccagcaca atcgcgttcg gatgtgtgaa gctctcaatc agtggttgca
    14941 ggctggtaac aggcttttcc gtcgggtgat ggcggttgcc tgaatatttc cagcccagca
    15001 cgtccggcag cgggttttgt ggcagacgtg gacggccttt tgccaggatg taggcgcatt
    15061 cgtggcgata gcccacatat gcggccttcg atgtgtagtt tttggtgaag acgagatgcc
    15121 ctacaacgct gaatccggcg cgtttccagg ctgccataaa gcggtcaata cggttccagc
    15181 catagaaact caccatcagc gcgtcttttt tgagtacgcg gtacatctca ttactggcag
    15241 gttgcagcca gtcatcgtta acgtcaccgg caatggtgcg cccctgacgg tcacgaaaac
    15301 cgacgagata cggcgggtcg gtgaggatga aatcgacagc attgtccgga aacgtggcca
    15361 taactcgcac gcagtcaccc aggataaaac gggacataaa agcctccttc tcttacagag
    15421 ggcaccggaa gggcacccct gtggtgcccg ttccccgctg cctgtgggct gttcaccgcg
    15481 tccggtaacg ggtcaggccg accgtggaat accgcagccg tcgtcttttt gacggcaagg
    15541 ctatgccgcg aaagcggctt gaagggcgtt catcagaaaa gggcgacggg ctggcgtaca
    15601 ctgtcaccga cgccagccgg gagcggtaaa agcgcccgtc ccgtgcccgc cggacgcggg
    15661 acactgacca caggcagcgt ttatgccgcg ccgcgtcagc cggcgcagta aaaagaggag
    15721 cccggcgaca tcctttaccg gaaaagaaac gggcggttgc ccgcccgtct ccgtcatact
    15781 tcatccgcca taatgcgcca ccttgtcgac tatccacgca ggtccggtga cttccggcag
    15841 ctcatcgtca tctgtcgggg aagaccattc cagttccccc cagagcacat caacaagctc
    15901 tccacgctca tatcgctgcc agccacagaa attgcagccc tgttcggcgt accagtgctc
    15961 cagcgtgcag ccgaaacggc ggcttaattc tgcaacaacg ttgctttccg gctgacacca
    16021 cggcgtgtcg aaatccacct gaatgaagtt ttctccctga cggctgatat tcacctcata
    16081 ccccacaggc cacttcacgc catactgttc cgtataccag tgatacgcag aaggaacacc
    16141 attcagaaca ccaccgttaa agccattcac ttcaacatcc aggcgggtgg gcagaaccgt
    16201 cagcaggtca aacggcatgg ttttttccgg cagtacattg tcacacagac ggttccacca
    16261 caccgataca tcctcgttac tccagaagcc acaccagtca cctcttttgg cggtgaaatg
    16321 cacggtgatg gtttccctga cctcatccgg taatccttcc cagcgacgct ggccagtacc
    16381 actctgcagc cagagttcat gcagcatcag gcagttttgt tcatccagta gcacaccgtt
    16441 ctgcaggtgt gtcagccagc gggtgaacgc gatattctcc ggcgatacaa caccacgtcc
    16501 ggcagccgtc actccggggc acggttcaaa ctgcacatct tcagtggtct gcagaagtcc
    16561 ggcacttccg gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat aaaacggtgt
    16621 gactgcaccg ctggccagtc gtttaatctc agcaatctgt gccggttcac cagaaaaatg
    16681 catacgattt gagcaccagt ttggcatggg aattctcctc tcacatgcgg cctccatcag
    16741 gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc ccgaagccgc
    16801 agcgcgtgga ggaggaacga cgaaacgtga ccgggtgagg aggccggcga cagcgacaca
    16861 cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tggttaagta gtttccgtct
    16921 cagtttgtga cgcaaaatga gaaaccagca acgactcaaa ttgagttaca gcaagaacac
    16981 aaagagactc taaaagagtc acattacttg acattaaccc aaaatgagac acaataagaa
    17041 ataacaaaaa ctcaaaaaga tgcatcaaca taaaagatgc tcaatttgag acaaacacaa
    17101 actcaaaacg aggcaaaaag aatgctaaaa gtatcatgcg atgatggttc aactaacgta
    17161 aaactggcct ggcttgaaga tggcgaggta agaaccagtc tgtcaggaaa cagctttaaa
    17221 gaaggctgga acccgggact gtttaatgcc ggcaaagttt acaattacgt cgtggatgga
    17281 aaaaaataca cctatgattt gggcagcact gctgtcatag gtacaacaca tgtcagttat
    17341 caatacagca ccaccaatct gcttgccata catcatgcac tgctgacatc cggccttcag
    17401 ccacaggatg tagaactgac agtaacatta cctgtaaccg aattctttga taatgacaat
    17461 caaccaaatg aagaaagaat agaaagaaaa aaggcaaatg ttctccgtga gatatctctg
    17521 aataaagggg aaacatttaa aattaaaaaa gttaatgtaa tgcctgaatc tttgccggct
    17581 gcatttgaat cactaaaaaa agataaagta aacaaacttg aacgcagcct gattatcgat
    17641 cttggaggaa ccactctgga ttgtggttta attctcggtg catttgaagg catttctgaa
    17701 atccgtggct attcagaaat cgggacatcc cgcataactc atacc
//
LOCUS       DBFIFS010000080        17419 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.080, whole genome shotgun sequence.
ACCESSION   DBFIFS010000080 DBFIFS010000000
VERSION     DBFIFS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.080"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..106)
                     /locus_tag="N7A07_002422"
     CDS             complement(<1..106)
                     /locus_tag="N7A07_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HGU2477070.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMND"
     gene            complement(233..964)
                     /gene="rlmB"
                     /locus_tag="N7A07_002423"
     CDS             complement(233..964)
                     /gene="rlmB"
                     /locus_tag="N7A07_002423"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU2477071.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1145..3586)
                     /gene="rnr"
                     /locus_tag="N7A07_002424"
     CDS             complement(1145..3586)
                     /gene="rnr"
                     /locus_tag="N7A07_002424"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU2477072.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3625..4050)
                     /gene="nsrR"
                     /locus_tag="N7A07_002425"
     CDS             complement(3625..4050)
                     /gene="nsrR"
                     /locus_tag="N7A07_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU2477073.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGLMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4255..5553)
                     /gene="purA"
                     /locus_tag="N7A07_002426"
     CDS             complement(4255..5553)
                     /gene="purA"
                     /locus_tag="N7A07_002426"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU2477074.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5657..5854)
                     /gene="yjeT"
                     /locus_tag="N7A07_002427"
     CDS             complement(5657..5854)
                     /gene="yjeT"
                     /locus_tag="N7A07_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU2477075.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5936..6940)
                     /gene="hflC"
                     /locus_tag="N7A07_002428"
     CDS             complement(5936..6940)
                     /gene="hflC"
                     /locus_tag="N7A07_002428"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU2477076.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6943..8202)
                     /gene="hflK"
                     /locus_tag="N7A07_002429"
     CDS             complement(6943..8202)
                     /gene="hflK"
                     /locus_tag="N7A07_002429"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU2477077.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8288..9568)
                     /gene="hflX"
                     /locus_tag="N7A07_002430"
     CDS             complement(8288..9568)
                     /gene="hflX"
                     /locus_tag="N7A07_002430"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU2477078.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9645..9953)
                     /gene="hfq"
                     /locus_tag="N7A07_002431"
     CDS             complement(9645..9953)
                     /gene="hfq"
                     /locus_tag="N7A07_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU2477079.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10039..10989)
                     /gene="miaA"
                     /locus_tag="N7A07_002432"
     CDS             complement(10039..10989)
                     /gene="miaA"
                     /locus_tag="N7A07_002432"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU2477080.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10982..12829)
                     /gene="mutL"
                     /locus_tag="N7A07_002433"
     CDS             complement(10982..12829)
                     /gene="mutL"
                     /locus_tag="N7A07_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU2477081.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12839..14176)
                     /gene="amiB"
                     /locus_tag="N7A07_002434"
     CDS             complement(12839..14176)
                     /gene="amiB"
                     /locus_tag="N7A07_002434"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU2477082.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14195..14656)
                     /gene="tsaE"
                     /locus_tag="N7A07_002435"
     CDS             complement(14195..14656)
                     /gene="tsaE"
                     /locus_tag="N7A07_002435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU2477083.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14628..16175)
                     /gene="nnr"
                     /locus_tag="N7A07_002436"
     CDS             complement(14628..16175)
                     /gene="nnr"
                     /locus_tag="N7A07_002436"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU2477084.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16174..17313
                     /gene="queG"
                     /locus_tag="N7A07_002437"
     CDS             16174..17313
                     /gene="queG"
                     /locus_tag="N7A07_002437"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU2477085.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKRLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17296..17349)
                     /locus_tag="N7A07_002438"
     CDS             complement(17296..17349)
                     /locus_tag="N7A07_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477086.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3946 a         4974 c         4362 g         4137 t
ORIGIN      
        1 aatcattcat tttaataatt aatgcgttct cgctatttgt tacatcaata ttttgttcag
       61 gtacagtttg cagcgccgtc gccagcgcca acggattcca tgtcatatat tattccatat
      121 agattaagtt taaatattaa taaaatgaat atttgcaata cgtaattatc ttaccagcta
      181 tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc
      241 gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa
      301 cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca
      361 taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctttgataga
      421 gcgtatgatc cgcctcacct gccgtaccga cgatccagat attctcttcc tgcaacatac
      481 gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc gcgccgcagg
      541 ctactttttt cgccgtggcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcgt
      601 gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacac
      661 cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt
      721 cctggtactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc
      781 ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctgg gactcaaggg
      841 cgtggatcag cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa
      901 aacgttcagg ggcgcgctcc agcagggcct gcactgcatg gatgccgtaa atcatttcgc
      961 tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggtctacatg
     1021 atccctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca
     1081 tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctgttt gaagagggta
     1141 atgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcagctattt
     1201 tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgct gctttcggct
     1261 tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct
     1321 gacgacgctt gccgcctttt ttacctgcat cgcctttttt cgctttctcg cgcgccgttt
     1381 taccgacgtt gcgcggtgcg cgttcgctgg agatcaggct aaagtcgatt ttgcgctcgt
     1441 ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc
     1501 tggattcacc catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg
     1561 aagagacatg gaccagccca tcaatgaaca agtcgtccag acggacgaag aagccaaagc
     1621 cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac
     1681 acttcagcca gtcagacaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac
     1741 agtgctgacc caattgcagc atctcttcca tcgaataatg gtagccaccg gtttcagtgg
     1801 tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg tgcagcatca
     1861 ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccaggccaa
     1921 agtgaccacg gttttccgga tcgtaaatcg cctgtttcat cgagcgcagc agcatggttt
     1981 gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagctccgcg tagtcgcgcg
     2041 gttccggctt gttaccgccc ggcagttcca gccccagctc tgccagcact gaacggaaag
     2101 aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt
     2161 tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca attaacttgt
     2221 gtgcgtcgtt acgctgagtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga
     2281 acttcgcttc ttcgctctca aatgagatcc cgccgcgttc ttcacgggct ttatccagca
     2341 ctttatagag gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca
     2401 gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc
     2461 tcatcaccgc ttcgtagaat ttgtagccag tcaggcggcc tttcgacgaa accgtcatct
     2521 cgcacaccat acacaggcgg tctacctgcg gattgagcga gcacagaccg ttagagagca
     2581 cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtacca cggttacgcg
     2641 cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcagca atcgcgaccc
     2701 ataaacgcca gccgccgccg cgttttttct cgcagtaaac cgcatcgtca aagtcacggg
     2761 cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg
     2821 cttcttccgg cacttcttct ttcagcccag caacctgttg ctcaacagcc tgcggccaga
     2881 tgtacggaat ttcatgggta cgcagagcga tatcaaccgc catgcccgtg cccatattgt
     2941 cgcccagcac ttcgacgatt ttacccaccg ctttggtacg gcgagtcggg cgttgggtca
     3001 gttcaacaac caccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga
     3061 tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt
     3121 agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac
     3181 gacctttacg gtcagcaccc agcggctgag ccagcacctg atcgccatga atgcaggttt
     3241 tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt
     3301 agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat
     3361 agcactgacg acgagtaaag accagttgac catcgcgctc catcgcgcgc aggcggcgac
     3421 gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc
     3481 tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat
     3541 tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt
     3601 tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt
     3661 ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc
     3721 cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca
     3781 attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc
     3841 cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg
     3901 actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac
     3961 ttcagaaata ctggtcatca gcccttctgg caatgacgcc atgtagatca gcgcacgtaa
     4021 tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa
     4081 tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg
     4141 gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag
     4201 caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc
     4261 gtcgaacggg tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc
     4321 gatcggtaca ccggtcagct cttcaatgcg cttgatgtag ttcagcgccg cctgcggcag
     4381 gccgctacga tctttcacgc cgaaggtgga ttcagaccag cccggcatgg tttcgtaaat
     4441 cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagtcactt cacgaccatc
     4501 cggcatacgg taagccacac agagtttcac ctctttcagg ccatccagaa cgtccagttt
     4561 agtcaggcag aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc
     4621 cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac cctgcttgca
     4681 gaggaactcg ccagtttcat caaacagttc agtcgggaac ggacctgcac ccacacgagt
     4741 ggagtaagct ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc
     4801 ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc
     4861 gatatccagc agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc
     4921 ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat
     4981 cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg
     5041 gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag
     5101 gtcgccaaca cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg
     5161 accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac
     5221 atgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat
     5281 gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc
     5341 gttaccgatg atgctagtta cattctcgcg gagaatacct gatggaataa gatggagaac
     5401 ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac
     5461 aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca
     5521 ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa
     5581 tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga
     5641 tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg
     5701 caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag
     5761 agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa
     5821 ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac
     5881 caccgacatc cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac
     5941 gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca
     6001 tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt
     6061 agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt
     6121 caccgcgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt
     6181 cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta
     6241 ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt
     6301 tgatctgctt aattcgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt
     6361 tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg
     6421 cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg
     6481 cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct ttcacgtcca
     6541 ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt
     6601 gcgaaatgtc gccccccccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt
     6661 tgatgtaaga gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt
     6721 tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga
     6781 aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt accttaccaa
     6841 aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta
     6901 ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg
     6961 gtagtcgtta cgctgcgcat tggcgcggcg ttggtccata atatcgccct gactggtgga
     7021 cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt
     7081 gctggcaccg ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa
     7141 cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa
     7201 cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata
     7261 ttccggcaga agtttagcaa aacgcgccac ttcaccctga gcttccagga tggtctgggc
     7321 cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac
     7381 ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat
     7441 cgcatcgtca aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc
     7501 cagcagcgtg atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc
     7561 gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac
     7621 tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata
     7681 cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt
     7741 ctcgtccgac gtcagcatca caccagaagc tgccagttca cgcacggctt ccacgttcac
     7801 cggtttgact tcgtcgataa acgtcggttt ccagttcaga cctggctcaa ccagatggct
     7861 gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact
     7921 ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg
     7981 cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag
     8041 accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc
     8101 gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg
     8161 gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg
     8221 ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc
     8281 tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt
     8341 caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt
     8401 ctattgcctg aagctgataa aaacgacttc tcagacgccc ttcctgcggt ggcagacgca
     8461 atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta
     8521 tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat
     8581 caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga
     8641 tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac
     8701 gcacatccgc cgcatcaatc acatgcagca gcaatgtggc ttgccgcgtc tcttgtaacg
     8761 tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg
     8821 caagtacggt ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg
     8881 caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt
     8941 taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt
     9001 gccgcccctg ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc
     9061 gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccaa
     9121 tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgtgta gccagatggc
     9181 gcagctgcgc cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa
     9241 tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac
     9301 gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga
     9361 cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt
     9421 tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa
     9481 actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct
     9541 gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa
     9601 cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc ggtttcttcg
     9661 ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta
     9721 ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc
     9781 gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa
     9841 gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt
     9901 tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct
     9961 tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata
    10021 cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg
    10081 cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt
    10141 tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta
    10201 tgagatttcg cctccaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg
    10261 caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga
    10321 agccaacatc tgatgaaaac gctgctcaat tcgttgatgg agcagttcac ggctcgccgg
    10381 ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc
    10441 cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc
    10501 atttggatga atccttgctg ccgcaaccgg atcgatctcc tgaagttgac gatgcaatga
    10561 ctcccaacct tgctctgccg cctgttgctc aattctggcc cgtacttccg ggtctgccga
    10621 cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa
    10681 cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa
    10741 atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc
    10801 gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt aaataagggc
    10861 agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg ctaacgccgt
    10921 tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat
    10981 atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac
    11041 ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg
    11101 cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa
    11161 tattgccagg ttcgaatacg gactgcttcg ctaggtagcc tatcagttca ggaatcaaga
    11221 tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat
    11281 ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct aatgccgatt
    11341 tttcttcgcc agaaactttt agccgcaacg gaatcagcaa cggctgggcg caaacgggcg
    11401 cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc aaggctaaaa
    11461 gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc
    11521 gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc gcttttggtt
    11581 tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt
    11641 aacctggctg cgcattcggc cagggggcag ccggacgact acctgatgct ggcgcaggag
    11701 tgtagcgcgg agctactggc tcacgaactg ccggttcagc aaagtgattg cgccccgccg
    11761 ccacgcggtt ttccggaatg ggacgcggtg caggttgggg ttcatcgtcc agcggtagcg
    11821 gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaag tcatgcacca
    11881 gacgcgactg atgaaaacgc acttcgtgtt tggcggggtg cacgttgacg tccacctgat
    11941 gcgggtcgat ctccagatac aacacaaatg caggttgctg atcggccccc agtttgtctt
    12001 cacaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga ccattcacgt
    12061 agcaatactg aatttccgcc agtgcgggcg tggtgtgatt tggatcggcc acccagccgc
    12121 gcagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc
    12181 cacaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcaccgcg cggtactggc
    12241 gtacgatttt gccgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agtgcgatgc
    12301 ggcggatgat ttcatcgata tggttaaatt cggttttctc ggtgcgcagg aatttgcgcc
    12361 gcgccggggt gttgtagaac aggtccagta cctccagcgt cgttcccacc ggatgcgccg
    12421 ccggtttaac cgtcacgtcc atatcgcgcc cttcggcata ggcctgccag gcttcctgct
    12481 gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct
    12541 caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg
    12601 tggcatgacg cgccagcgcc agcgccagct cgtctttttt gataccacag ccgttatcac
    12661 gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg
    12721 catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg accacctcac
    12781 ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt
    12841 agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc gttgcaccct
    12901 gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg taaatggctt
    12961 ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga
    13021 taaaaccggt ttcgaccagt actgatggga tatccggcga gcgcagaacg ccaaggctgg
    13081 cgtgttctgg tcgacgttta tgtatttcgc caatgcgttg caactgactg atcatactgg
    13141 tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc accgcctggc
    13201 ttaaataggg gtcagactga ctgttcgcca gcacatcacc cgccccgccc agcagctccg
    13261 actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga cggttagaga
    13321 gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca
    13381 cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc gagataaagt
    13441 aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc aaagtacgca
    13501 atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc
    13561 cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccattgttag
    13621 ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt
    13681 tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgtag
    13741 gcgtttcaac gcgtttcaca accacaggcg gcggaggagg cggtggcggc gcatcggcgt
    13801 taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct tcggttttac
    13861 cattttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc gttccggagc
    13921 gaatcgcctt caccagatta ttaccgctga acaacaacgg cagtccctga atcacgcccg
    13981 tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat
    14041 cgccaataaa actcaacgtt atccgcgcct gttggttgcc gttagaaacc tgaatatcag
    14101 agagcgtcgc ggcacccacc tgcgcgcaca gcagcagcaa cgtcgctacc aaccaatttc
    14161 tgatgcgata catcatccca ccacctttca aaggttaacc ggctaaacgt gccagcaaca
    14221 attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa
    14281 tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc cactccacca
    14341 ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc tcctcgggat
    14401 cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta tagggttcga
    14461 ccagcgtata agtggggctt ttgacattac cctgatggcc caaagcctgt aaaaagcccc
    14521 ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg gttgcgccat
    14581 cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca gggagcggaa
    14641 ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg ctgtagcgtg
    14701 gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct
    14761 gccgcaccgt gcgcgacaca gcctgcacag gctgcatcat acggcgacag tttttgccca
    14821 agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgcttgc catgcctgca
    14881 tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct
    14941 ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca
    15001 ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg
    15061 atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc
    15121 cacaacatcg gtttgcgaaa attctcaact ttttgcaggg cttttttccc ccactcttgc
    15181 tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag
    15241 tccatagtca gttcatgcac catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc
    15301 tcactgcggg tcagtactcg gactaaacca gcaccagcac gtagcgccgc ttcccccgtc
    15361 atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg
    15421 cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc tgccgaaaac
    15481 cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga gtcaaaatgc
    15541 agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctggtttcag cgcaataaaa
    15601 gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg
    15661 ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg gtcgattaac
    15721 tggctaatgg attcgcgggg tgcttgctgc aagccggtac cgagcagcgc atcaacaatc
    15781 agagctaccg actcgggcca gacaatattc gaagcatgga tctcgcctcc cgcgtttaac
    15841 catgcctcgc gtgccagcgc ggcctcttcc ggcaacggtt tatcgctctc ctgggccaac
    15901 aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg
    15961 ttattaccat gaccgcacaa caccagccag tggcgggcgt caggatacgc cgaacgacac
    16021 acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag ccccagcgca
    16081 tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt gccagacggt gtgtggtata
    16141 cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag
    16201 ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc
    16261 gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac
    16321 ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttattg
    16381 cccggtacgc tgcgcgtgat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt
    16441 gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct gggccgtgac
    16501 tatcacaaac gtctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt
    16561 gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct
    16621 gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt
    16681 tcgttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga tcaaccagtc
    16741 gaggaaggat gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc
    16801 gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga actggaaggg
    16861 gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg cgatgactgc
    16921 cagcttatct gcccgtggaa tcgctattca caactcacta cagaagacga tttcagcccg
    16981 cgtaagccgc tacacgcacc ggaactcatt gagttattcg cctggagcga agagaagttt
    17041 ttaaaagtca cggaaggttc ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat
    17101 atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc gctcgagagt
    17161 cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgatggc gcagcaaatc
    17221 gagaggcgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg
    17281 gtaattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata
    17341 aaaacgcatt gcaattcaag acgaataaaa tcgtcaagtg atcgactcga caaaacgaaa
    17401 tcttaatttt taaattatc
//
LOCUS       DBFIFS010000081        17361 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.081, whole genome shotgun sequence.
ACCESSION   DBFIFS010000081 DBFIFS010000000
VERSION     DBFIFS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.081"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            224..1891
                     /locus_tag="N7A07_002439"
     CDS             224..1891
                     /locus_tag="N7A07_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU2477087.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1901..3160
                     /locus_tag="N7A07_002440"
     CDS             1901..3160
                     /locus_tag="N7A07_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU2477088.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3171..3986
                     /locus_tag="N7A07_002441"
     CDS             3171..3986
                     /locus_tag="N7A07_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2477089.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3983..4876
                     /locus_tag="N7A07_002442"
     CDS             3983..4876
                     /locus_tag="N7A07_002442"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU2477090.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(5015..6082)
                     /gene="purK"
                     /locus_tag="N7A07_002443"
     CDS             complement(5015..6082)
                     /gene="purK"
                     /locus_tag="N7A07_002443"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU2477091.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6079..6588)
                     /gene="purE"
                     /locus_tag="N7A07_002444"
     CDS             complement(6079..6588)
                     /gene="purE"
                     /locus_tag="N7A07_002444"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU2477092.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(6706..7428)
                     /gene="lpxH"
                     /locus_tag="N7A07_002445"
     CDS             complement(6706..7428)
                     /gene="lpxH"
                     /locus_tag="N7A07_002445"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU2477093.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(7431..7925)
                     /gene="ppiB"
                     /locus_tag="N7A07_002446"
     CDS             complement(7431..7925)
                     /gene="ppiB"
                     /locus_tag="N7A07_002446"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU2477094.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8099..9484
                     /gene="cysS"
                     /locus_tag="N7A07_002447"
     CDS             8099..9484
                     /gene="cysS"
                     /locus_tag="N7A07_002447"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU2477095.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(9520..10041)
                     /locus_tag="N7A07_002448"
     CDS             complement(9520..10041)
                     /locus_tag="N7A07_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2477096.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(10149..10361)
                     /gene="ybcJ"
                     /locus_tag="N7A07_002449"
     CDS             complement(10149..10361)
                     /gene="ybcJ"
                     /locus_tag="N7A07_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU2477097.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(10363..11229)
                     /gene="folD"
                     /locus_tag="N7A07_002450"
     CDS             complement(10363..11229)
                     /gene="folD"
                     /locus_tag="N7A07_002450"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU2477098.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            11709..12251
                     /gene="fimA"
                     /locus_tag="N7A07_002451"
     CDS             11709..12251
                     /gene="fimA"
                     /locus_tag="N7A07_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2477099.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            12471..13163
                     /gene="fimC"
                     /locus_tag="N7A07_002452"
     CDS             12471..13163
                     /gene="fimC"
                     /locus_tag="N7A07_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU2477100.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            13194..15803
                     /locus_tag="N7A07_002453"
     CDS             13194..15803
                     /locus_tag="N7A07_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU2477101.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            15816..16823
                     /gene="fimH"
                     /locus_tag="N7A07_002454"
     CDS             15816..16823
                     /gene="fimH"
                     /locus_tag="N7A07_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU2477102.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            16834..17349
                     /gene="sfmF"
                     /locus_tag="N7A07_002455"
     CDS             16834..17349
                     /gene="sfmF"
                     /locus_tag="N7A07_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU2477103.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
BASE COUNT         4181 a         4266 c         4665 g         4249 t
ORIGIN      
        1 tagttagaat ttatttgata atccgctcgc ttttaacctg gtttttaaaa caaccacgct
       61 tattaaaaaa taacacgtaa tagcatggcg gttatttgaa ttcttttgtt aataatccct
      121 gtgtgatatt catcacctta tttactcgtt gtcatcgata ccgtaatcgc cacattaaca
      181 ctgctcgtgc aattgccatg ggtgcaattt ttaaggagtt gttatgatcc acgcctttat
      241 taaaaaaggg tgttttcagg attcggtcag tttaatgatt atttcacgaa aactcagcga
      301 atcagaaaat gttgatgatg tttccgtaat gatgggtacg cccgccaata aagcgttatt
      361 agataccaca ggtttctggc atgacgattt taataacgcc acgccgaacg atatttgcgt
      421 ggcaattcgt agcgaagcgg cggatgcggg gatcgcgcag gcgattatgc agcagcttga
      481 agaggcgcta aaacaactgg cgcaggggtc aggcagcagc caggcgttga cgcaggtgcg
      541 tcgctgggac agtgcctgtc agaaattacc cgatgccaat ctggcgctga tttcagtggc
      601 tggcgagtat gcggcggagc tggcgaatca ggcgctggat cgcaacctca acgtgatgat
      661 gttctccgat aacgtcacgc tggaagatga aatccaactt aaaacccgcg cgcgggaaaa
      721 aggcttgctg gtgatggggc cggactgcgg tacgtcgatg attgccggca cgccgctggc
      781 ttttgctaac gtgatgccgg aaggcaacat tggcgtcatt ggcgcttccg gtaccgggat
      841 tcaggagctg tgctcgcaga ttgcgctggc aggggaggga attactcacg cgattggcct
      901 tggcggccgc gacctcagcc gtgaagtggg cggcatcagt gcgctgacgg cgctggaaat
      961 gctcagtgca gacgagaaaa gcgaagtgct ggcatttgtt tcaaaaccac ctgccgaagc
     1021 ggtgcgtctg aaaattgtta atgccatgaa agcaaccggc aaaccgacgg tggcgctgtt
     1081 tttaggttat accccggcgg tggcccgcga cgagaatgtc tggtttgcct cctcgctgga
     1141 tgaggccgca cgcctggctt gcctgctttc acgcgttacg gcgcgacgta acgcgataat
     1201 gcctgccagc agcggattta tttgcggttt gtataccggc ggtacgctgg ctgccgaagc
     1261 ggcgggatta cttgccggac accttggcgt ggaagccgat gatgcccatc atcacggcat
     1321 gatgctggac gccgatggcc accagattat tgacctcggc gatgatttct acaccgtcgg
     1381 gcgtccccat ccgatgatcg acccaacctt acgcaaccag ttaattaccg atctcggcgc
     1441 taaaccgcaa gtgcgtgtgc tgctgcttga tgtcgtgatt ggcttcggtg cgaccgccga
     1501 tcctgccgcc tcgctggtaa gcgcctggca aaaagcctgt gctgcgcgtt cagacaatca
     1561 accactgtat gccattgcca cggtgacagg cactgaacgt gacccgcaat gccgctcgca
     1621 gcaaatcgcc acgctggaag atgcggggat tgcggtcgtg agttcgctac cggaagccac
     1681 cttgctggcg gcagcgttaa ttcatccgct ctcgcctgcc gcacagcaac acacaccgtc
     1741 attactggaa aacgtcgccg tgattaacat cggattacgc agttttgcgc tggagctaca
     1801 aagcgccagc aaaccggttg tgcattacca atggtcgcca gtcgccggcg gcaataaaaa
     1861 actggctcgt ttattagaac gtttgcaata aggggttctc atgtttacat cagtggcgca
     1921 agccaatgct gcggttatcg aacaaattcg tcgcgctcgt ccacactggc tggatgtgca
     1981 accggcttct tcacttatca gcgaactaaa cgagggcaaa acactgcttc acgccgggcc
     2041 gccaatgcgc tggcaggaga tgaccggacc catgaaaggg gcgtgcgtgg gcgcatgtct
     2101 gttcgaaggt tgggcgaaag atgaagcgca ggcgctggca atactggagc agggggaagt
     2161 gaacttcatt ccttgtcacc atgtgaatgc cgtcgggcca atgggcggta ttacttctgc
     2221 cagtatgccg atgctggtgg tcgagaacgt gaccgacggc aaccgggcgt actgcaacct
     2281 caacgaaggt atcggcaaag tgatgcgttt tggcgcttac ggcgaagatg tcctgactcg
     2341 ccatcgctgg atgcgcgatg tgttaatgcc agtattaagc gcggcgctgg ggcgcatgga
     2401 gcgcggtatc gatcttactg cgatgatggc gcagggcatt acgatgggcg atgagttcca
     2461 tcaacgcaat attgcttcct cagcactgtt aatgcgtgcg ctggccccac aaattgctcg
     2521 cctcgatcat gataaacagc acatcgcgga agtgatggat ttcctcagcg tgaccgatca
     2581 gttcttcctc aacctcgcga tggcttactg caaggcggcg atggatgctg gcgcgatgat
     2641 ccgcgcaggc agcatcgtca cggcaatgac ccgcaacggc aatatgttcg ggattcgggt
     2701 aagcgggctg ggcgaacgct ggtttactgc gcctgtaaac actccgcaag gtctgttttt
     2761 caccggcttc tcgcaggagc aggcgaaccc ggatatgggc gatagcgcga ttaccgaaac
     2821 ctttggtatc ggaggtgcgg caatgatcgc agcgcctggc gtaacgcgct ttgtcggtgc
     2881 gagtggcatg gaagcggcca gggcggtatc tgaagagatg gcggaaattt accttgaacg
     2941 caatatgcag ttgcagatcc caagctggga ttttcagggc gcgtgcctgg gactggacat
     3001 tcgtcgcgtg gtagaaaccg gcattacgcc actcatcaat accggtatcg cccataaaga
     3061 ggcggggatc gggcagattg gcgcaggcac cgtgcgggca ccgctggcgt gctttgaaca
     3121 ggcgctggaa gcactggctg aaagcatggg gattggttga ggaacgcgta atgacgatca
     3181 tccatcctct gcttgccagt agtagcgcac cgaattatcg ccagtcctgg cggttagcgg
     3241 gagtgtggcg gcgggcgatt aacctgatga cggaaagcgg cgaactgtta acgctgcatc
     3301 gtcagggtag tggtttcggc cccggaggat gggtgcttcg ccgtgcgcaa ttcgatgcgt
     3361 tatgcggtgg attatgcggc aatgaacgac cacaggttgt ggctcaaggg attcgcctcg
     3421 ggcgtttcac ggttaaacag ccacagcgtt attgtttgct gcgtattacg ccgcctgcgc
     3481 atcctcaacc acttgcagct gcatggatgc aacgcgcgga ggaaaccggg cttttcgggc
     3541 cactggcgtt ggcggcaagc gatccgctgc ctgctgagtt acgccagttt cgtcactgtt
     3601 ttcaggccgc gctcaatggc gttaagaccg actggcggca ctggctgggt aaaggccccg
     3661 gattaacgcc aagtcatgat gacacgctga gcggaatgct gctggcggcc tggtattatg
     3721 gtgctttaga tgcgcgctcc ggtcgtccgt tttttgcctg ttccgacaat ctgcaactcg
     3781 ttaccacagc ggtgagcgtc agttatttac gttatgccgc gcaaggatat ttcgcctcgc
     3841 cactcctgca ctttgttcat gctctgagtt gcccgaaacg taccgctgtt gcgattgatt
     3901 cgctgctggc gctggggcat acgtcagggg cagatacact gctggggttc tggcttggcc
     3961 aacaattatt acaaggaaaa ccatgaaaac actggttgta gctcttgggg gcaacgcctt
     4021 actccagcgc ggtgaggcgc tgacggcaga aaatcaatat cgcaatatcg ccagtgctgt
     4081 acccgcgctg gcgcgcctgg cccgttctta tcggctggcg attgttcacg gcaacgggcc
     4141 gcaggtgggg ctgctggcat tgcagaatct ggcgtggaaa gaggtagaac cgtatccgct
     4201 ggatgtgctg gtggcagaaa gtcaggggat gattggctat atgctggcgc agagtttgag
     4261 cgcacagccg cagatgccgc ctgtcacgac ggtgcggacg cgcattgaag tttcgcctga
     4321 tgatccggcg tttttgcagc cagagaaatt tattggtccg gtttatcagc cagaagaaca
     4381 agaggcactg gaagcggctt acggctggca gatgaaacgt gatggtaaat atttgcgccg
     4441 ggtggtggcg tctccgcaac cgcgtaaaat tctcgacagc gaagccatcg agttgttgct
     4501 caaagagggg catgtggtga tttgcagtgg cggcggcggt gtgcctgtga cggatgacgg
     4561 agcagggagt gaagcagtga ttgataaaga tctcgccgct gcgttgctcg ccgagcaaat
     4621 taacgccgat ggactggtga tcctcaccga tgctgatgcg gtatatgaaa actggggaat
     4681 gccgcagcaa cgtgccattc gccatgccac accggatgag ttagcgccat ttgccaaagc
     4741 cgatggttcg atggggccga aggtaacggc ggtgagtggt tatgtcagaa gccgtggtaa
     4801 acccgcgtgg attggggcgt tatcgcgaat tgaagagacg ctggcgggcg aagcggggac
     4861 ctgtatttcg ctgtagtgat gagagcatcc ggcaatcggc gcaccatgcc tgatgcgacg
     4921 cttgacgcgt cttatcaggc ctacaatggg taccggatcg gtgtaggccg gataaggcgt
     4981 tcacgccgca tccggcaatc aaatgagcac caggttaaca gaacttactc tgcgcccaca
     5041 tcacgccgct ggcatattcc ggcggcagca gcgggatcaa ggcttccagc gtcgcggaca
     5101 gacgcgatgt gtcgctgtcg gtcaaattca gatgccccac tttacggccc ggacggactt
     5161 ctttgtcgta ccagtgcaga tgcaccagcg gcagtttcag ccagtcataa ttcacatcgc
     5221 taccaatcag attgatcatc accgacggat tattcaccac tggttgcggt aacggcagat
     5281 cggtaatcgc ccgcagatgt agctcaaact ggctgatgct ggcaccgttt tgtgtccagt
     5341 gaccgctgtt atgcacacgc ggtgccagtt cgttgatcag cagaccttgc ggggtgacaa
     5401 aacactccat cgccatcacg cccacatagc ccagctcctg cataatcgcc gacagcatct
     5461 cttcggcttg cgcttgctgc tgcgcattgg cctgcggaaa agcgacgctg gtgcgcaaaa
     5521 taccgtcctg atgcaggtta tgcgtcagcg gataaaacac ggtgctgcca tcaaagcagc
     5581 gcgcgccaac cagcgacact tcaccagaga agtttatgcc ctgctcgaca atacattcgc
     5641 cgtagcattc agccggtaac tgctcggttt catcggcgcg taaacgccat tgaccgcgtc
     5701 cgtcatagcc accagtgcga cgcttaacaa tcgccagctc acctaaacgc tcaaacaccg
     5761 caggccactc gctgcgttcg gcaagtaact gccacggtgc agtcggcagg tggagcttat
     5821 cgaaaagctg cttctgagtc agacggtcag caataatcgg gaacacatcg cggttcacaa
     5881 aggccggatg gcgcgccagc tcgcgggtta atgctgtttc cggccagcgt tcgatctcag
     5941 cggtaatcac gctttgttga aaaggcaccg ccgccggttc agcgtccagc ccgaccggcc
     6001 agacagcaat gcctaacggt tcgcctgcct gacgcagcat acggcctaac tgcccgttac
     6061 cgaggacgca aacctgtttc atgccgcacc tcgcgggtcc gggttttcca gcacttcatc
     6121 agtttgtgct ttgcgccagt cattcagacg ctggtgcagt tctttatcat gagtcgcaag
     6181 aatttgtgct gccagtaatg ccgcgtttgc cgcgccagct ttaccaatcg ccagcgtacc
     6241 caccggaatg ccgcgtggca tttgtacgat ggagtagagg ctatcgacac cgctcagtgc
     6301 ggcgctctgt actggcacgc ccagcactgg caccagcgtt ttggcggcaa tcatgcctgg
     6361 caggtgcgct gcgccgcctg cgcccgcaat aatcacctga taaccgttct cttcggcact
     6421 ttcggcgaag ctgaatagtt tatcgggggt gcggtgagca gaaaccactt caacgtggtg
     6481 cgggacattc aggatttcga agatttcggc ggcgaactgc atggtagccc agtcgctttt
     6541 ggaccccatc acgatggcga cacgcgccgg attattgcgg gaagacatgc gtcttaaaac
     6601 tcctgtggtg cacaactctc ggctttagag ggcacagaga atagcacgga aagagagcaa
     6661 ggaaaacggt tgcgtggctg tgaaatcagc aaagttgcgg gttttttaaa acggaaaatg
     6721 aatcagctca acgtcatccg ccgtgacttt caccatcgaa ccttccgtat gccaggcacc
     6781 cagtaccacg cgaaaagcag gttgctgatt ggcgataagt tcatgcaccg ccgggcgatg
     6841 ggtatgcccg tggatcagcc attgcacctg atgtttttcc atcgcactga ctaccgcgtt
     6901 ttggttaacg tccatgatcg ccagcgattt actgctgttg gcttctttac tgttcgcgcg
     6961 cattcgcacg gcaatgcgtt tgcgcacaaa caacggcaga gcgaggaata gcgtttgcag
     7021 ccagggtttg tggactttgg tgcgaaaagc ctgataaccc gcgtcatcgg tgcacagcgt
     7081 gtcgccatgc ataatcagca cccgacgacc ataaagttcg agcacttttt cttccggcaa
     7141 taacgtcatg ccactttcac gggcaaagcg tttgccgagc agaaaatcac ggttgccatg
     7201 aatgaaataa cagggaacgc cggaatcgga caccgctttg atcgctgccg ccatctggcg
     7261 atggagtggg ttgggatcgt cgtcgccaat ccatgcttca aacagatcgc caagaatata
     7321 cagcgcgtcg gccttgcggg cttctcccgc taaaaaacgc agaaaaccgg cggtgatcgc
     7381 cggttcttcc acgcagagat gaagatctgc aataaagagt gtcgccacga ttactcgcta
     7441 acggtcacgc tttcaatgat aacgtcttct tttggcacgt cctggtgcat accgctacga
     7501 ccggttgcaa cacctttgat tttgtctacc acgtccatgc cttcaaccac ttcagcaaac
     7561 acgcagtagc cccaaccttg caggctttcg ccagagaagt tcaggaagtc gttatcaacc
     7621 acgttgatga agaactgtgc agttgcagag tgcggagcct gagtacgggc cattgccagc
     7681 gtaccacggg tatttttcag gccgttgttg gcttcgtttt tgatcggttc tttggtggct
     7741 ttttgtttca tgcccggttc aaaaccgccg ccctgaatca taaagccgtt gataacacgg
     7801 tggaaaatgg tgttgttgta aaaaccttcg cggcagtagt ccaggaagtt tttaactgtt
     7861 tcaggtgctt tatcgtcaaa agttttgatg acaatatcgc cgtgattggt gtggaaagta
     7921 accatttttg catcctgttc cgtttgattg gtgcttcaac ccagttcggg tcatatatag
     7981 ggtggtgtta tagcataacc gcacgatcgg atcatcacgc aatgtatgct gattcgcgcg
     8041 ggaaatatgg gtattatacg caactcaatt acccacacat gtctaaacgg aatcttcgat
     8101 gctaaaaatc ttcaatactc tgacacgcca aaaagaggaa tttaagccta ttcacgccgg
     8161 ggaagtcggc atgtacgtgt gtggaatcac cgtttacgat ctctgtcata tcggtcacgg
     8221 gcgtaccttt gttgcttttg acgtggttgc gcgctatctg cgtttcctcg gctataagct
     8281 gaagtatgtg cgcaacatta ccgatatcga cgacaaaatc atcaaacgcg ccaatgaaaa
     8341 tggcgaaagc tttgtggcgc tggtggatcg catgatcgcc gaaatgcaca aagattttga
     8401 tgcgttgaac attctgcgcc cggatatgga gccgcgcgcg acgcaccata tcgcagaaat
     8461 tattgaactc actgaacaac tgatcgccaa aggtcacgct tatgtggcgg acaacggcga
     8521 cgtgatgttc gacgtcccga ccgatccaac ttatggcgtg ctgtcgcgtc aggatctcga
     8581 ccagctgcag gcaggcgcgc gcgttgacgt ggtcgacgac aaacgcaacc caatggactt
     8641 cgttctgtgg aagatgtcga aagagggcga accgagctgg ccgtctccgt ggggcgcggg
     8701 tcgtcctggc tggcacattg aatgttcggc aatgaactgc aagcagctgg gtaaccactt
     8761 tgatatccac ggcggcggtt cagacctgat gttcccgcac cacgaaaacg aaatcgcgca
     8821 gtccacctgt gcccatgatg gtcagtatgt gaactactgg atgcactcgg ggatggtgat
     8881 ggttgaccgc gagaagatgt ccaaatcgct gggtaacttc tttaccgtgc gcgatgtgct
     8941 gaaatactac gacgcggaaa ccgtgcgtta cttcctgatg tcgggccact atcgcagcca
     9001 gctgaactat agcgaagaga acctgaagca ggcgcgtgcg gcgctggagc gtctctacac
     9061 tgcgctgcgc ggcacagata aaaccgttgc gcctgccggt ggcgaagcgt ttgaagcgcg
     9121 ctttattgaa gcgatggacg acgatttcaa caccccggaa gcctattccg tgctgtttga
     9181 tatggcgcgt gaagtaaacc gtctgaaagt agaagatatg gcagcggcga atgcaatggc
     9241 atctcacctg cgtaaacttt ccgccgtatt gggcctgctg gagcaagaac cggaagcgtt
     9301 cctgcaaagc ggcgcgcagg cagacgacag cgaagtggct gagattgaag cgttaattca
     9361 acagcgtctg gatgcccgta aagcgaaaga ctgggcggcg gcagatgcgg cgcgtgaccg
     9421 tcttaatgag atggggatcg tgctggaaga tggcccgcaa gggaccacct ggcgtcgtaa
     9481 gtaattgcgc tattgccgga tgcgagtttt cgcatccggt tatcgtctgc gccaccacaa
     9541 cattcccatc agtagcatcc ccggcaacca cacccacatc aattcagaaa taatcacctg
     9601 atgcccgtac gctgtggtgt aacgagacaa tgcaaacggc gcgactttaa tcacctgcca
     9661 gggagcgaaa aagcgttcat ctgaccacgg ccacagccag ccaacccctt taccgccagt
     9721 ggttaccgaa tccagcaagc tgtgcgatag caacgagacg gttaaaaaca gccagcagcg
     9781 aatcagccca gccctgaacc atcgacgtcc aatcagcaca cataacagcg ggacaacaaa
     9841 cgcaaacacc agcgaatggg taaacccgcg atgaccaaaa acattgccgt aagcaacgcc
     9901 aaaattaaac gacaagacgt cggcatcggg cagcatcgcc aggatgattc cggcaaataa
     9961 caggcgcgga gggatgactt tcgaacccaa ccctaaacca atgcataggg gaacggcggc
    10021 gtgcgtaata acggttggca tgatggtcgc ttcggcaaaa tgtcgatgct atcagcatgg
    10081 atgaacgggg cgtagagggc aaaagtctga aaagagaacc ggcctgttga tacaggccgg
    10141 gaaagggatc aggcaacaac ctgtacgctg tgacctgcaa aactcactgt ctgcccggcg
    10201 acgattttgc agcgtttgcg cgtttcaacc gcgccgtcga ctttcacctg gccttcggca
    10261 atcgcgattt tcgcctgcgc gccgctttcg ctccagcctt ccagtttcag caagtcgcac
    10321 agctcaacgt gcggatgttt acctaaagaa aatgtcgcca tgttactcac cctgtggatc
    10381 atgatattca acgcacgcct gtagcgtgtt ttcaatcagc gtggcaaccg tcatcgggcc
    10441 aacgccgccc ggaacaggcg taatgtatga ggcgcgttta gccgcgtctt caaacacgac
    10501 gtcgcccaca actttgccat tttccagacg gttgatgccg acatcaatca caattgcgcc
    10561 ttctttgatc cagtcaccag gaataaagcc cggcttgccg acggcaacga tcaacagatc
    10621 ggcattttct acgtggtgac gcagattttt agtgaaacgg tgagtcacgg tggtggtgca
    10681 acctgcgagc aacagttcca tgctcatcgg gcggccaacg atattcgatg cgccaatcac
    10741 cacggcgttg aggccgaagg tatcaatgtt gtaacgctca agcagcgtga caataccgcg
    10801 cggggtgcag ggacgcagac gcggcgcgcg ctggcacaga cgaccgacgt tataaggatg
    10861 gaaaccgtct acgtctttgt ccgggtgtat acgttccagc actttgacgt tatcaatacc
    10921 cgccggtaac ggcagttgaa ccagaatgcc atcgatggtg ttgtcggcat tcagcgcatc
    10981 gataagctcc agcagctccg cttcgctggt ggtttccggg aggtcataag agcgggagac
    11041 gaacccgact tcttcacaag ccttacgttt gcttgcgaca taaatttgcg atgcagggtt
    11101 actacccacc agcacaacgg ccagtcctgg tgcccgcagt ccggctgcaa tacgcgcctg
    11161 aactttttga gcaacttcag agcgcacctg ctgcgcaatc gttttaccgt caataatctt
    11221 tgctgccatc agagagagga ttccatctgt tacgtaggtc gaaggggatg cgcctatttt
    11281 gtcagaagcg gggcgcgctg tcaggtttcg tttcagattt atcgcgtgaa gcgacctctt
    11341 gcgaaggtta ggcgcactgt cgctgaggtc gctgagattg aaagcttcat ttttcgtcca
    11401 tgatggcgtt gtaaatctgg aactgattta tttccttgtt taaggattaa gataatttaa
    11461 gaaatacctg acaatataaa aagaattttc agcctggtaa tttaccgctt caggtctata
    11521 tttgtgttga atatattttg cgcggaagta ttcatctaac ggggctctct attttttaga
    11581 atagagtgca tattttcaat taagacattc ttagaggata aaaaggaatt tactactatc
    11641 agtgtcttaa ataaagtaat cggttatata cggatgtgga gtcgataaat gagattgaag
    11701 gaatatatat gaaattaaga tttatttcgt ctgcgctggc tgccgcacta ttcgccgcta
    11761 cgggtagtta tgctgccgtt gtagatggcg gtacaattca ctttgaaggt gaactggtga
    11821 atgctgcctg ttcagtgaat actgactcgg cagaccaggt tgtcacactc ggtcaatatc
    11881 gtaccgatat tttcaatgct gttggtaata cctctgcatt aattccattc accattcagt
    11941 tgaacgactg cgatcctgtt gttgccgcta atgctgccgt tgcattttct ggtcaggctg
    12001 atgcaatcaa tgataattta ttggccattg catccagtac caatacaaca acagcaacgg
    12061 gtgtcggtat tgaaatactt gataatacat ccgcaattct caaacctgat gggaatagct
    12121 tctcaaccaa ccagaacttg atccccggga ccaacgttct tcatttttct gcacgttata
    12181 aaggcaccgg tacaagtgca tcagcagggc aagcaaatgc tgacgcgact tttattatga
    12241 gatacgaata atcaaaacca cgttgttttg aattatatat cacgtcttat aacaaagtaa
    12301 tgtaccggtt gtctgaagcg gtatggtggc aatgtaaatc gaaatcatgt tcactttgta
    12361 tcatgccgct ttattaaatg aaaagggaat gatgtgttgt aagaaaccaa agcaatcatt
    12421 tctttatatt tcttattttt gccgtcagga atacacaagg cgtattaact atgatgacta
    12481 aaataaagtt attgatgctc attatatttt atttaatcat ttcggccagc gtccatgctg
    12541 ccggagggat cgcattaggt gccacgcgta ttatttatcc cgctgatgct aaacagactg
    12601 cggtatggat tagaaatagc cataccaatg agcgttttct ggtcaattcg tggattgaaa
    12661 acagcagcgg tgtaaaagaa aagtcattca tcattacacc gccactattt gttagtgaac
    12721 ccaaaagcga aaatactttg cgtattattt acaccggtcc accgctggca gcagatcgtg
    12781 agtctctgtt ctggatgaat gttaagacga ttccttcggt agataaaaat gcattgaacg
    12841 gcaggaatgt tttgcaactg gcgattttat cgcgcatgaa gttatttctc cgtccaattc
    12901 aactacaaga attacccgca gaagcgccgg atacactcaa gttttcgcga tccggtaact
    12961 atatcaatgt tcataatcca tcaccttttt atgtcaccct ggttaactta caagtgggca
    13021 gccaaaagtt ggggaatgct atggctgcac ccagagttaa ttcacaaatt cccttaccct
    13081 caggagtgca gggaaagctg aaatttcaga ccgttaatga ttatggttca gtaaccccgg
    13141 tcagagaagt gaacttaaac taaccgaatc atctgacaat atcagagcta attatgaaaa
    13201 tacccactac tacggatatt ccgcagaggt atacctggtg tctggccgga atttgttatt
    13261 catctcttgc cattttaccc tcctttttaa gctatgcgga aagttatttc aacccggcat
    13321 ttttattaga gaatggcaca ttcgttgctg atttatcgcg ctttgagaga ggtaatcatc
    13381 aacctgcggg cgtgtatcgg gtggatctct ggcgtaatga tgagttcatt ggttcacagg
    13441 atatcgtatt tgaatcgaca acagaaaata caggtgataa atcaggtggg ttaatgccct
    13501 gttttaacca ggtactcctt gaacgaattg gccttaatag cagtgcattt cccgagttag
    13561 cccagcagca aaacaataaa tgcatcaatt tactgaaagc tgtacctgat gccacaatta
    13621 actttgattt tgcagcgatg cgcctgaaca tcactattcc tcagatagcg ttgttgagta
    13681 gcgctcacgg ttacattccg cctgaagagt gggatgaagg tattcctgct ttactcctga
    13741 attataattt caccggtaac agaggtaatg gtaacgatag ctattttttt agtgagctca
    13801 gcgggattaa tattggcccg tggcgtttac gcaacaatgg ttcctggaac tattttcgcg
    13861 gaaatggata tcattcagaa cagtggaata atattggcac ctgggtacag cgcgccatta
    13921 ttccgctgaa aagtgaactg gtaatgggag acggcaatac aggaagtgat attttcgatg
    13981 gtgttggatt tcgtggtgta cggctttatt cttccgataa tatgtatcct gatagccagc
    14041 aagggtttgc cccaacggta cgtgggattg cccgtacggc ggcccagcta acgattcggc
    14101 aaaatggttt tattatctat caaagctatg tttcccccgg cgcttttgaa attacagatt
    14161 tgcacccgac atcttcaaat ggcgatctgg acgtcaccat cgacgaacgc gatggcaatc
    14221 agcagaatta cactattccg tattcaacag tgccaatttt acaacgcgaa gggcgtttca
    14281 aatttgacct gacggcgggc gattttcgta gcggtaatag tcagcaatca tcgcctttct
    14341 tttttcaggg cacggcactc ggcggtttac cacaggaatt tactgcctac ggcgggacgc
    14401 aattatctgc caattacacc gcctttttat tagggctggg gcgcaacctc gggaactggg
    14461 gcgcagtgtc gctggatgtg acccatgcgc gcagtcagtt agccgacgac agtcgtcatg
    14521 agggggattc cattcgcttc ctctatgcga aatcgatgaa caccttcggc accaattttc
    14581 agttaatggg ttaccgctat tcgacacaag gtttttatac ccttgatgat gttgcgtatc
    14641 gtcgaatgga ggggtacgaa tatgattacg attatgacgg tgagcatcgg gatgaaccga
    14701 taatcgtgaa ttaccacaat ttacgcttta gccgtaaaga ccgtttgcag ttaaatattt
    14761 cacaatcact taatgacttt ggctcgcttt atatctctgg tacccatcaa aaatactgga
    14821 atacttcgga ttcagatacc tggtatcagg tggggtatac cagcagctgg gttggcatca
    14881 gttattcact ctcattttcg tggaatgaat ctgtagggat ccccgataac gaacgtattg
    14941 tcggacttaa tgtttcagtg cctttcaatg ttctgaccaa acgtcgctac acccgggaaa
    15001 atgcgctcga ccgcgcttat gcctccttta acgccaaccg taacagcaac gggcaaaata
    15061 gctggctggc aggtgtaggt gggaccttac tggaaggcca caacctgagt tatcacgtaa
    15121 gccagggtga tacctcgaat aatgggtata cgggcagcgc cacggcaaac tggcaggccg
    15181 cttacggtac gctgggggtc gggtataact acgaccgcga tcaacatgac gttaactggc
    15241 agctgtctgg cggtgtggtc ggacatgaaa atggcataac gctgagccag cctttagggg
    15301 ataccaatgt tttgattaaa gcgcctggcg caggcggtgt acgcattgaa aatcaaactg
    15361 gcattttaac cgactggcgc ggctatgcgg tgatgccgta tgccacggtt tatcggtata
    15421 accgtatcgc gcttgatacc aatacgatgg ggaattccat cgatgttgaa aaaaatatta
    15481 gcagcgttgt gccgacgcaa ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg
    15541 tgcgggcgct cattaccgtt acccagggcg gaaaaccggt gccgtttgga tcactggtac
    15601 gggaaaacag taccggaata accagtatgg tgggtgatga cgggcaagtt tatttaagtg
    15661 gtgcgccatt gtctggtgaa ttactggttc agtggggaga cggcgcgaac tcacgctgca
    15721 ttgcgcacta tgtattgccg aagcaaagct tacagcaagc cgtcactgtt atttcggcag
    15781 tttgcacaca tcctggctca taaaggaaat tatcaatgaa gataatctgc agattattat
    15841 tggcgatggc atgtttgtgt ctggcaaaca tatcctgggc tactgtttgt gcaaatagta
    15901 ctggcgtagc agaagatgaa cactatgatc tctcaaatgt ctttaatagc accaataacc
    15961 agccagggca gattgttgtt ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt
    16021 gtccacccgg cacgctggtg aattatacat accgtagtta tgtcactaac tttattgttc
    16081 aggaaactat cgataattat aaatatatgc aattaaatga ttatctatta ggtgcgatga
    16141 gtctggttga tagtgtgatg gatattcaat ttcccccgca aaattatatt cgtatgggaa
    16201 cagatcctaa cgtttcgcaa aaccttccgt tcggggtgat ggattctcgt ttgatatttc
    16261 gtttaaaggt tattcggccc tttattaaca tggtggagat ccccagacag gtgatgttta
    16321 ccgtgtatgt gacatcaacg ccttacgatc cgttggttac acctgtttat accattagtt
    16381 ttggtggccg ggttgaagta ccgcaaaact gcgaattaaa tgccgggcag attgttgaat
    16441 ttgattttgg tgatatcggc gcatcgttat ttagtgcggc agggccgggt aatcggcctg
    16501 ctggtgtcat gccgcaaacc aagagcattg cggtcaaatg tacgaatgtt gctgcgcagg
    16561 cttatttaac aatgcgtctg gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca
    16621 atcaggattt aggttttatt gtcgccgatc agaacgatac gccgatcacg cctaacgatc
    16681 tcaatagcgt tattcctttc cgtctggatg cagctgcggc agccaatgtc acacttcgcg
    16741 cctggcctat cagtattacc ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt
    16801 atttacgcgt cgattatcaa tgaggtacgg agaatgagaa gagtattctt tagctgtttc
    16861 tgcgggttac tgtggagttc cagtggatgg gcagctgacc ctttaggaac gattaatatc
    16921 aatttgcacg gtaacgttgt tgatttctcc tgtaccgtaa acacagcgga tattgataag
    16981 acggtagatt taggcagatg gcctacgaca caattactga acgctggcga taccacggca
    17041 ctcctccctt ttagcctgcg gctggaggga tgtcctccgg gttcagttgc gattttattt
    17101 acgggaacgc cggcatctga tgccaacctg ctggctctgg atgatcccgc aatggcacaa
    17161 accgtcgcca tcgaattacg taatagcgat cgctcccggc tcgcactggg ggaggcgagc
    17221 ccgactgagg aagtagatgc aaatggcaat gtcacactaa acttttttgc caattatcga
    17281 gcgttagcca gcggtgttcg gccaggtgtg gcgaaagcgg atgcgatatt tatgatcaat
    17341 tataattaat attatattaa t
//
LOCUS       DBFIFS010000082        17322 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.082, whole genome shotgun sequence.
ACCESSION   DBFIFS010000082 DBFIFS010000000
VERSION     DBFIFS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.082"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1388)
                     /gene="djlB"
                     /locus_tag="N7A07_002456"
     CDS             complement(<1..1388)
                     /gene="djlB"
                     /locus_tag="N7A07_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU2477104.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFI"
     gene            complement(1385..2092)
                     /gene="ybeR"
                     /locus_tag="N7A07_002457"
     CDS             complement(1385..2092)
                     /gene="ybeR"
                     /locus_tag="N7A07_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2477105.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2256..3233
                     /gene="ybeQ"
                     /locus_tag="N7A07_002458"
                     /pseudo
     CDS             2256..3233
                     /gene="ybeQ"
                     /locus_tag="N7A07_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3303..3785)
                     /gene="ybeL"
                     /locus_tag="N7A07_002459"
     CDS             complement(3303..3785)
                     /gene="ybeL"
                     /locus_tag="N7A07_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU2477106.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4021..6603
                     /gene="leuS"
                     /locus_tag="N7A07_002460"
     CDS             4021..6603
                     /gene="leuS"
                     /locus_tag="N7A07_002460"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU2477107.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6618..7199
                     /gene="lptE"
                     /locus_tag="N7A07_002461"
     CDS             6618..7199
                     /gene="lptE"
                     /locus_tag="N7A07_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU2477108.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7199..8230
                     /gene="holA"
                     /locus_tag="N7A07_002462"
     CDS             7199..8230
                     /gene="holA"
                     /locus_tag="N7A07_002462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU2477109.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8232..8873
                     /gene="nadD"
                     /locus_tag="N7A07_002463"
     CDS             8232..8873
                     /gene="nadD"
                     /locus_tag="N7A07_002463"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU2477110.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8897..9508
                     /gene="cobC"
                     /locus_tag="N7A07_002464"
     CDS             8897..9508
                     /gene="cobC"
                     /locus_tag="N7A07_002464"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU2477111.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9768..10085
                     /gene="rsfS"
                     /locus_tag="N7A07_002465"
     CDS             9768..10085
                     /gene="rsfS"
                     /locus_tag="N7A07_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU2477112.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10089..10556
                     /gene="rlmH"
                     /locus_tag="N7A07_002466"
     CDS             10089..10556
                     /gene="rlmH"
                     /locus_tag="N7A07_002466"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU2477113.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10587..12488
                     /gene="mrdA"
                     /locus_tag="N7A07_002467"
     CDS             10587..12488
                     /gene="mrdA"
                     /locus_tag="N7A07_002467"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU2477114.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12491..13603
                     /gene="mrdB"
                     /locus_tag="N7A07_002468"
                     /gene_synonym="rodA"
     CDS             12491..13603
                     /gene="mrdB"
                     /locus_tag="N7A07_002468"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU2477115.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13614..14702
                     /gene="rlpA"
                     /locus_tag="N7A07_002469"
     CDS             13614..14702
                     /gene="rlpA"
                     /locus_tag="N7A07_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU2477116.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14842..16053
                     /gene="dacA"
                     /locus_tag="N7A07_002470"
     CDS             14842..16053
                     /gene="dacA"
                     /locus_tag="N7A07_002470"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU2477117.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16163..16426
                     /gene="ybeD"
                     /locus_tag="N7A07_002471"
     CDS             16163..16426
                     /gene="ybeD"
                     /locus_tag="N7A07_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU2477118.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16527..17168
                     /gene="lipB"
                     /locus_tag="N7A07_002472"
     CDS             16527..17168
                     /gene="lipB"
                     /locus_tag="N7A07_002472"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU2477119.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4344 a         4355 c         4426 g         4197 t
ORIGIN      
        1 aagataaaaa tatagaaaat aattttaatg atgcccagac agcctggccc accatcgtca
       61 acgggcattg gctgcggttg cggctccggt tcaacagttt gcttctcggt aaaattcgcc
      121 gcctgaatta ataatcgacg aataattaat gcttccagtg gacaacgatt ttctgcatca
      181 agacggctta cgatcggcat taatgattct tctgtaggtt ggctggcctg cactaattgt
      241 ttggcaacta gatataatgg ccataaggta tgttctgtat cacgcgtgag tgagatccgc
      301 ggcttacatc ctttgtgcaa aaccatctcg accagtgaac gccccagagc agagagtttt
      361 tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa
      421 agtagcggtt ttccctggtc gttaaccagt tcacgagctt caatcatcat caccactaat
      481 gggtaataat cgactcgatg ctgacggcac cattgcaaca ttaaagcatc cgcctgagtg
      541 gagggatact cgtgccagta gtcacaaagt tctgccagca ggctttcgcc ttctccacaa
      601 tagacgcgtt gcgcgtattc ataataacgc gcatcttcat tatcaggttc agcttcccgc
      661 cacgaccggg cgtaatccag gagttcgggg attcccaggc gacctgcact ataccagcgg
      721 agtaaatttc tgtgcagttt tgcatcatcg gggataatca ctgcgcgatg ttgcgctaaa
      781 tacaatgtca gccattctgg atgcgatgac caaatccggg cgaacatttg gtaaaaatcg
      841 atggtgtcat tttgcacggc aacgggcaga tgcagaatga tctggaagtt aaaaacattt
      901 cccttcgcaa tggcatataa aaaggattcc agttcttctt cgtctatttc cccgacgttt
      961 tcctcctgca accaccgcaa tctttctgct aataacacca cgcactcgaa ggataaatgg
     1021 gcagtgttca tggctattgt gcacagcgac cagcgtaatt catcaatctc attcatcgag
     1081 caataattta attgctgaat gaatcgctgc caggcggagg gcagaaaacg ttcactatca
     1141 gaggcaagta acgcacgaaa ggcgagcaga atttcatgtt ctgctacctc atattcttct
     1201 ggttgccaaa cagatttagc aggcgactgc gcaatccgga gcgcttcctc atacgcctga
     1261 cgaagttgtt taaaaccctg cggatcggtt tctggatgaa aggacggtaa cagcgccaga
     1321 taagcgcggc ggataatatc gacatcagtc gtttcctcta tgttgaggat cttccagcaa
     1381 tttttcatag gtcgctcata tccttaagcg tttccgggca tgtggggtag tcagccagtg
     1441 gttgccaggg taaggtcgcg taatatgcat cgtcactggc aaataattga taaaacatat
     1501 cattgccgga atcgtcgtat tctttacgcg ctaagaattc acgtaataaa ggcagattat
     1561 catcttcggg agactgccaa tattgccgac caaaggtata accggcaaag tattgccgcc
     1621 agttactata ataacgtaga gcccgtagat gaatacgcga ctggatccat agactttctt
     1681 cttcactcaa ccagttatta agtacgccca ttcgacttaa aaaacccatc cggacataat
     1741 cccatgcttt aattcctccc tcaccacagc attcggctgt gctggcgaca aattgcgcat
     1801 aagcctgacc ttgctcattt aattcagcaa gatagtcacg ccattcacat ggagaataac
     1861 gaaaccagcg gtgataaaac cctgccaacc gcgcggcatg accgttatcg atcattcggt
     1921 gaattgtgcg cagcaggtca tcacggtcat caattcccca ggaggaagaa aggtcaaaaa
     1981 agcgtgtacc taaatacatt ttgggtgatg aatgcgaagg taaacagcat tcattgcgta
     2041 tttccatcgg agtggaaagt gcaaacaata ttttttgcga ctccatgtcc atatgattta
     2101 tccttaaata ttcattgaat gaatattagc cgcattgcga cgctcgttta tttatacttt
     2161 tctgacgcga cataatacgc ataaaataag gcttgcgcaa aaatgaaaca cataaaaatt
     2221 caatatattt tatctacaat cataaggaaa tgataatgat tttcacgtca agttgctgcg
     2281 ataatttatc aatagatgag attatcgaac gtgctgaaaa aggcgattgc gaggctcaat
     2341 atattgttgg gttttattat aatcgcgata gcgcaattga ctctccggac gacgaaaagg
     2401 ccttttacta gctgaagctg gccgctgagc aaggtcattg tgaagcacag tattccttag
     2461 ggcaaaagta taccgaggat aaaagccgtc ataaagataa tgagcaagcc atcttctggc
     2521 tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa cgcccttggc tggatactgg
     2581 atcgtggaga agaccccaac tataaagaag cggttgcctg gtatcagata gccgcggaga
     2641 gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta cagaaatggc aacggagccg
     2701 cacaagacta tacgctggca tttttctggt acaaacaagc tgcattacaa ggccatagtg
     2761 acgcgcaaaa caatctggcc gatctttatg aagacggaaa aggcgttgct caaaacgaga
     2821 cactcgccgc attctggtat ttgaaaagcg cacagcaggg taatcggcat gctcagtttc
     2881 aaattgcatg ggattataac gctggcgaag gggtggacca ggactataag caagcgatgt
     2941 actggtatct gaaggctgcc gctcagggga gcgtcggcgc ttacgtcaac atcggttata
     3001 tgtataaaca cggacaaggc gttgagaaag attatcaggc tgcctttgaa tggtttacga
     3061 aagccgctga atgcaatgac gccactgcct ggtataacct ggccattatg tatcactacg
     3121 gagaaggaag acctgtcgat ctccgacagg ctctcgacct gtatcgtaaa gttcagtcat
     3181 ccggaaccag agatgtcagt caggaaattc gtgagactga agatttactg tagagataaa
     3241 aacaagggcc ggggaatttc attcggccca tattctcttt attgctgtgc gaaatcagac
     3301 tattacggct caaacgggcg tctctggaac tggtcatgac cacatttcgg gcatagcgtc
     3361 agcacttccg gtgtgtagat cgggagatgg aagtgacatt tctcgcagac cagatttccc
     3421 agcccgacca cttctccgct gtgataaacc ccatgatgat tgaggtcctg gaaaacttcg
     3481 cgccattcaa gctgcgtttt atcggtgatg tctgccagct cctgccacaa gctttcttta
     3541 atcacccgca taaagacgct gtcagattct tctttcaggc tctcttcata gctcatggcg
     3601 aactcttcca ggtcacgtct gacagctcgc gtcagctcat cgacctcggt tcgcgttaac
     3661 tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg
     3721 ccattgcgca ggcgttcgct cagtgacgca accagttcac ggtaatattg agcaaccttg
     3781 ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc tttgttcctt
     3841 gctttgctaa tacgcccaca aactctgtaa attaatgtat gaataatccg ggcatcattt
     3901 caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc tatgcggatc
     3961 tgaaaaacca cctctaacgc tacttttgta gccgtaatga aaacaggacc actggctgcc
     4021 atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag
     4081 aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg
     4141 cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac
     4201 gtgatcgccc gttaccagcg catgctgggc aaaaacgttc tgcagccgat cggctgggac
     4261 gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg
     4321 acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac
     4381 tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc
     4441 accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg
     4501 aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat
     4561 accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac
     4621 gagctgctca acgatttgga taaactggat cactggccag acaccgttaa aaccatgcag
     4681 cgtaactgga tcggtcgttc cgaaggcgtg gaaatcacct tcaacgttaa cgactatgac
     4741 aacacgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg
     4801 gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc
     4861 tttattgacg aatgccgtaa caccaaagtt gctgaagctg aaatggcgac gatggagaaa
     4921 aaaggcgtca atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt
     4981 tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagtaatggc ggttccgggg
     5041 cacgaccaac gcgactacga gtttgcctct aaatacggcc tgaacatcaa accggttatc
     5101 ctggcggctg acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg
     5161 ctgttcaact ctggcgagtt caacggtctt gaccatgaag cggccttcaa cgccatcgcc
     5221 gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgtgactgg
     5281 ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt
     5341 accgtaatgc cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg
     5401 gacggcatta ccagcccgat taaagcagat ccacagtggg cgaaaactac cgttaacggt
     5461 atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat
     5521 gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac
     5581 tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac
     5641 ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg
     5701 aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac
     5761 ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt
     5821 atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg
     5881 tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac
     5941 accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa
     6001 tccggcgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt gtacgagcat
     6061 actgcgaaag gtgatgttgc ggcactgaac gttgatgcac tgactgaaga tcagaaagcg
     6121 ctgcgtcgcg atgtgcataa aactatcgct aaagtgaccg atgatatcgg ccgtcgtcag
     6181 accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcaccg
     6241 accgatggcg agcaggatcg cgctctgatg caggaagcac tgctggccgt tgtccgtatg
     6301 cttaacccgt tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc
     6361 gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg
     6421 ctggtcgtgg tgcaggttaa cggtaaagtc cgtgctaaaa tcaccgttcc ggtggacgca
     6481 acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat
     6541 ggcgttactg tacgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc
     6601 taagcgcggg aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa
     6661 tcaccgccgg gtgtggctgg catctgcgtg acaccacgca ggttccttcc actatgaagg
     6721 tcatgatcct ggactcaggc gatccgaacg ggccattaag ccgtgcggtg cgtaaccagt
     6781 tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc
     6841 tgcgtttggg tgctgtatca atctcacagg ataccgcgtc ggttttccgt aatggtcaaa
     6901 ctgcggaata tcagatggtg atgactgtta gcgcgtcggt attgattcct ggacgagata
     6961 tctacccgat tagcaccaaa gtcttccgtt cattcttcga taacccgcaa atggcgttag
     7021 cgaaagataa cgagcaggaa atgatcatta aagagatgta cgaccgtgct gccgaacagc
     7081 tgattcgtaa gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt
     7141 cgactacaac ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat
     7201 gattcggttg tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta
     7261 tcttttactt ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt
     7321 agctgcggca caaggattcg aagaacacca cactttttcc attgatccca acactgactg
     7381 gaatgcgatc ttttcgttat gccaggccat gagtctgttt gccagtcgac aaacgctatt
     7441 gctgttgtta ccagaaaacg gaccaaacgc ggcgatcaat gaacaacttc tcacactcac
     7501 cggacttctg catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca
     7561 agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac
     7621 accggagcag gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga
     7681 actggatgac gcggcgaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct
     7741 ggctcaggca ctggagcgtt tatcgctgct ctggccagac ggcaaactga cattaccgcg
     7801 cgttgaacag gcggtgaatg atgccgcgca tttcactcct tttcattggg ttgatgcttt
     7861 gttgatggga aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag
     7921 cgaaccggtt attttgttgc gcacattaca acgtgaactc ttgttactgg ttaacctgaa
     7981 acgccagtct gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa
     8041 ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt cagccgcagt tacgtcaggc
     8101 cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa gattacagtc agtcagtgtg
     8161 ggcagagttg gaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat
     8221 cgacggttga tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact
     8281 atggtcatct aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgagtcacaa
     8341 tcatccctaa taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtacagcgta
     8401 aacacatgct tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc
     8461 taaagcgcaa tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag
     8521 ggccggacgt gccgctggcg tttattattg gtcaggattc attgctgacc tttccgacct
     8581 ggtacgaata cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt
     8641 acccacttga aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata
     8701 acccggaaga tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt
     8761 ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatca tgtgatgatt
     8821 tattgccgga accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt
     8881 cgttacggag ggcatgatgc gactgtggtt aattcgtcat ggcgaaacac aagcgaatgt
     8941 cgatggtctg tacagcggtc atgcgcccac acccctgacc gcacgcggta ttgagcaagc
     9001 gcaaaatctg catacactgc tagatgatgt ttcctttgac ctggttttat gcagtgaact
     9061 ggaacgggca cagcataccg cgcgactggt tctcagtgac cgccagctcc ccgttcatat
     9121 catccctgaa ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct
     9181 catgcaagaa gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgctatccc
     9241 cacgaacggt gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct
     9301 tagtgaatat cagcactatc agaatatttt aatcgtcagc catcagggcg tacttagtct
     9361 gttaattgct cgtttaattg gcatgcctgc cgagtctatg tggcattttc gcgttgacca
     9421 ggggtgttgg agcgccattg atataaacca aaaattcgcg acgctacgcg tcctcaatag
     9481 ccgtgccatc ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg
     9541 accattgaca gccccgggca ggctgatatt ctccgcagcc agactttttc cgccagacac
     9601 gactttgtag aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga
     9661 tagcccactt tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt
     9721 atactgacag accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag
     9781 cactccagga ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct
     9841 tagacgttca gggcaaatcc agcatcactg actgcatgat catctgtacg ggtacgtcca
     9901 gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt
     9961 taccgctcgg agtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg
    10021 tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga
    10081 gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa
    10141 ccggttttac cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa
    10201 ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg
    10261 agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc
    10321 cctgggatac gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg
    10381 tcagtctact gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc
    10441 agagctggtc gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag
    10501 agagtctgta ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag
    10561 ggagctttga gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg
    10621 cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga
    10681 ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga
    10741 cccgctctaa tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct
    10801 acgaccgtaa cggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc
    10861 cggagaaagt cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga
    10921 ccgatgacga tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcca
    10981 ttccggtgaa aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc
    11041 gttttccggg tgttgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt
    11101 tgacccacgt catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata
    11161 atgacggcaa actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc
    11221 gttactatga agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc
    11281 gtgggcgtgt tatccgccag ttaaaagaag tcccaccgca agccggacac gatatttacc
    11341 tgacgctgga tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag
    11401 ctgtggtagt caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt
    11461 atgacccaaa cttgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg
    11521 acccgaatac accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag
    11581 ttaaacccta tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc
    11641 tgtttgaccc tggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa
    11701 aatggggcca cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct
    11761 tctatcaggt ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg
    11821 gttatggtca ttataccggt atcgacctgg cggaagaacg ttccggcaat atgcctaccc
    11881 gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg
    11941 gtatcggtca gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc
    12001 tgattaatga cggtatcgtg aaggttccgc atttgctgat gagcaccgcc gaagacggca
    12061 aacaggtgcc atgggtacaa ccgcatgaac cgcccgttgg cgatattcat tccggttact
    12121 gggagctggc gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata
    12181 aatactttgc cagcgcaccg tacaaaattg cggcgaaatc tggtaccgct caggtcttcg
    12241 gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca
    12301 aactgatgac cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg
    12361 agaacggtgg cgcgggtccg gcggttggta cgctgatgcg ccagatcctc gaccacatta
    12421 tgctgggtga taacaacacc gatctgcctg cggaaaatcc tgcggttgcc gcagcggagg
    12481 accattaatc atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga
    12541 tcccacaatg ctgctgattt tactggcatt gctggtttac agcgccctgg ttatctggag
    12601 cgccagcggt caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct
    12661 ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta
    12721 tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa
    12781 aggcgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa
    12841 aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt
    12901 gaagaacact ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca
    12961 gcctgacctg ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc
    13021 tggccttagc tggcgtctga ttggcgtcgc agtagtgctg gtagcggcgt tcattccgat
    13081 tctgtggttc ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctggaccc
    13141 ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc
    13201 cggcggatta cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc
    13261 cgaacgccat actgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat
    13321 tttgattctg ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag
    13381 agcgcaaacc acctttggtc gcgtcatggc tggcggctta atgctgatat tattcgttta
    13441 tgtcttcgta aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc
    13501 actggtcagt tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat
    13561 gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta
    13621 agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg
    13681 gtcagcaaca gacagtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa
    13741 ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc
    13801 gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg atttatccag gcgggactgg
    13861 cggcaatcta tgatgccgaa ccaggcagta acctgacggc ctctggcgaa gctttcgatc
    13921 cgacacagct gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta
    13981 acctggctaa cgggcgaatg atcgtggtgc gcattaatga tcgcggtcct tacggcaacg
    14041 accgcgttat ttcgctttct cgcgcagcag ctgaccgtct taacacgtca aacaacacca
    14101 aagttcgtat cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg
    14161 cttgtaccac agtcgccaaa cagacttacg ccctgcctgc acctcccgat ttaagcggtg
    14221 gcgcgggaac aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga
    14281 cgctaaaaag cgaagatccg accggcgcgc cggtaaccag cagcggtttc ctcggcgcac
    14341 caacgacctt agcgccaggt gtactggaag gcagcgaacc gacgcctgct ccacagcccg
    14401 ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg
    14461 cctcgcaaag cgccagcggc aactttatgg tgcaggtcgg ggccgtaagc gatcaggctc
    14521 gtgcgcaaca gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc
    14581 aaaatggcgc ggtctggcgg atccagcttg gcccattcgc caacaaagcc gaagccagta
    14641 ccttgcagca acgtttgcaa accgaagccc aattacagtc atttattacc accgcgcagt
    14701 agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg
    14761 aaagtcagat gcctgccggt agtgcatttg ctatagtagg gcactttttt taattccatc
    14821 acggatgtcg tagttcagac catgaatacc attttttccg ctcgtatcat gaagcgcctg
    14881 gcgctcacca cggctctttg cacagccttt atctctgctg cacatgccga tgacctgaat
    14941 atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg agtcctacat cctgattgac
    15001 tataactccg gcaaagtgct cgccgaacag aacgcagatg tccgccgcga tcctgccagc
    15061 ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa
    15121 gaaactgatt tagtcactat tggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa
    15181 ggttcttcgc tgatgttcct caaaccgggc atgcaggttc cggtttctca gctgatccgc
    15241 ggtataaacc tgcaatcggg taacgatgct tgtgtcgcca tggccgattt tgccgctggt
    15301 agccaggacg cttttgttgg cttgatgaac agctacgtta acgccctggg cctgaaaaat
    15361 acccacttcc agacggtaca tggtctggat gctgatggtc agtacagctc cgcgcgcgat
    15421 atggcgctga tcggccaggc gttgatccgt gacgtaccga atgaatactc gatctataaa
    15481 gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc gtaacggcct gttatgggat
    15541 aacagcctga atgtcgacgg catcaaaacc ggacacactg acaaagcagg ttacaacctt
    15601 gttgcttctg cgactgaagg ccagatgcgc ttgatttctg cggtgatggg cggacgtact
    15661 tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa
    15721 accgttaacc cactgaaagt aggtaaagag ttcgcctctg aaccggtttg gtttggtgat
    15781 tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc tgaccattcc gcgtggtcgc
    15841 atgaaagatc tgaaagccag ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa
    15901 aagaatcagg tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat cgaacaacgc
    15961 ccgctggttg tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat cattgattac
    16021 attaaattaa tgttccatca ctggtttggt taaaaattga acacttgaaa gtgtaatttc
    16081 cgtccccata tactaagcat cagtaaaaaa ctcccgcctt ctggcgggag ttgctattta
    16141 attacgttac gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac
    16201 tccttttact tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga
    16261 agtggtacag cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg
    16321 caactaccac tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact
    16381 gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt
    16441 acccggcccc tggtcgggta actcccctct cctcgctgtg atatactttt ctcccacttt
    16501 tactcattct ccacggagat gccgttttgt atcaggataa aattcttgtc cgccagctcg
    16561 gtcttcagcc ttacgagcca atctcccagg ctatgcatga attcaccgat acccgcgatg
    16621 atagtaccct tgatgaaatc tggctggtcg agcactatcc ggtattcacc caagggcagg
    16681 caggaaaggc ggagcacatt ttaatgcccg gtgatattcc ggtgattcag agcgatcgcg
    16741 gtgggcaggt gacttatcac gggccggggc aacaggtgat gtatgtattg cttaacctga
    16801 aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata
    16861 ccctggctga actgggtata gaagcgcatc ctcgggctga cgcgccaggt gtctatgttg
    16921 gggaaaagaa aatttgctca ctgggtttac gtattcgacg cggttgttca ttccacggtc
    16981 tggcattaaa cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg
    17041 ccggaatgga aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg
    17101 caccacgttt actggaaaat attttagcgc tactaaacaa tcctgacttc gaatatatta
    17161 ccgcttaatt tcatacctca atggcccaat ttacactggg tcattactca taacacctgc
    17221 taatgcttta catctccttc gcgtttgtat attttcttta tcacttgcag aaatgcgatg
    17281 ctacgccttg ctccgtaact caccccattg attaacttat tg
//
LOCUS       DBFIFS010000083        17266 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.083, whole genome shotgun sequence.
ACCESSION   DBFIFS010000083 DBFIFS010000000
VERSION     DBFIFS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.083"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            104..2779
                     /gene="adhE"
                     /locus_tag="N7A07_002473"
                     /gene_synonym="adhC"
     CDS             104..2779
                     /gene="adhE"
                     /locus_tag="N7A07_002473"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU2477120.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(3080..3697)
                     /gene="tdk"
                     /locus_tag="N7A07_002474"
     CDS             complement(3080..3697)
                     /gene="tdk"
                     /locus_tag="N7A07_002474"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU2477121.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            4301..4714
                     /gene="hns"
                     /locus_tag="N7A07_002475"
     CDS             4301..4714
                     /gene="hns"
                     /locus_tag="N7A07_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU2477122.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(4858..5766)
                     /gene="galU"
                     /locus_tag="N7A07_002476"
     CDS             complement(4858..5766)
                     /gene="galU"
                     /locus_tag="N7A07_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU2477123.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5968..6981)
                     /gene="rssB"
                     /locus_tag="N7A07_002477"
     CDS             complement(5968..6981)
                     /gene="rssB"
                     /locus_tag="N7A07_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU2477124.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(7073..7978)
                     /gene="rssA"
                     /locus_tag="N7A07_002478"
     CDS             complement(7073..7978)
                     /gene="rssA"
                     /locus_tag="N7A07_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU2477125.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            8091..8549
                     /locus_tag="N7A07_002479"
     CDS             8091..8549
                     /locus_tag="N7A07_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU2477126.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            8599..9441
                     /gene="purU"
                     /locus_tag="N7A07_002480"
     CDS             8599..9441
                     /gene="purU"
                     /locus_tag="N7A07_002480"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU2477127.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            9601..9685
                     /locus_tag="N7A07_002481"
     tRNA            9601..9685
                     /locus_tag="N7A07_002481"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9635..9637,aa:Tyr,seq:gta)
     gene            9730..9860
                     /locus_tag="N7A07_002482"
     ncRNA           9730..9860
                     /ncRNA_class="other"
                     /locus_tag="N7A07_002482"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            9895..9979
                     /locus_tag="N7A07_002483"
     tRNA            9895..9979
                     /locus_tag="N7A07_002483"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9929..9931,aa:Tyr,seq:gta)
     gene            10026..10157
                     /locus_tag="N7A07_002484"
     ncRNA           10026..10157
                     /ncRNA_class="other"
                     /locus_tag="N7A07_002484"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10204..10335
                     /locus_tag="N7A07_002485"
     ncRNA           10204..10335
                     /ncRNA_class="other"
                     /locus_tag="N7A07_002485"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(10519..11196)
                     /gene="narI"
                     /locus_tag="N7A07_002486"
     CDS             complement(10519..11196)
                     /gene="narI"
                     /locus_tag="N7A07_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU2477128.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(11196..11906)
                     /gene="narJ"
                     /locus_tag="N7A07_002487"
     CDS             complement(11196..11906)
                     /gene="narJ"
                     /locus_tag="N7A07_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU2477129.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(11903..13441)
                     /gene="narH"
                     /locus_tag="N7A07_002488"
     CDS             complement(11903..13441)
                     /gene="narH"
                     /locus_tag="N7A07_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU2477130.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(13438..17181)
                     /locus_tag="N7A07_002489"
     CDS             complement(13438..17181)
                     /locus_tag="N7A07_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU2477131.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4276 a         4574 c         4176 g         4240 t
ORIGIN      
        1 cttttgattt tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag
       61 cagatgattt actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt
      121 cgctgaactt aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt
      181 cactcaagag caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg
      241 aatcccactc gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt
      301 gatcaaaaac cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg
      361 tggtgttctg tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat
      421 tatttgcggt atcgttccga ccactaaccc gacttcaaca gctatcttca aatcgctgat
      481 cagtctgaag acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcgac
      541 caacaaagcg gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct
      601 gatcggctgg atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga
      661 catcaacctg atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg
      721 taaaccagct atcggcgtag gcgcgggcaa cactccggtt gttatcgatg aaactgctga
      781 tatcaaacgt gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg
      841 tgcttctgaa cagtctgttg ttgtcgttga ctctgtttat gacgcagtac gtgaacgttt
      901 cgcaacccac ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggacgttat
      961 cctgaaaaac ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga
     1021 actggcaggc ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt
     1081 tgatgaaagc gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc
     1141 taaagatttc gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gtggtatcgg
     1201 tcatacctct tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg
     1261 tcagaaaatg aaaacggctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg
     1321 tgacctgtat aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg
     1381 taactccatc tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa
     1441 gcgagctgaa aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc
     1501 cctgccaatc gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac
     1561 tgaccgcttc ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc
     1621 aggcgttgaa actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg
     1681 taaaggtgca gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg
     1741 ttccccgatg gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt
     1801 cgaagagctg gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat
     1861 gggcgtgaaa gcgaaaatga ttgctgtcac caccacttct ggtacaggtt ctgaagtcac
     1921 tccgtttgcg gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc
     1981 gctgaccccg gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct
     2041 gtgtgctttc ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact
     2101 ggcatctgag ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct
     2161 gccagcgtcc taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc
     2221 agcgactatc gcgggtatcg cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc
     2281 gcacaaactg ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg
     2341 taacgttatt cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta
     2401 tgaccgtccg caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc
     2461 accgggcgac cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa
     2521 ggctgaactg ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct
     2581 ggcgaacgtg gataaactgt ctgaagatgc attcgatgac cagtgcactg gcgctaaccc
     2641 gcgttacccg ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga
     2701 ttatgtagaa ggtgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa
     2761 agcgaaaaaa tccgcttaat cagtagcgct gtctggcaac ataaacggcc ccttctgggg
     2821 ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag ccttcatcat
     2881 atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg tcacaagtca
     2941 acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaactgc cgagaagggt
     3001 gtatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct
     3061 gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc
     3121 cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac
     3181 caataaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca
     3241 tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg
     3301 ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac
     3361 aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg
     3421 ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgctca tgttccgcac
     3481 gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta
     3541 tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag
     3601 tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta gacttacccg
     3661 cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat
     3721 gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac
     3781 agaagtggta tatgcgttct cccttatgaa gccttgcata atccttctga gctatcatta
     3841 caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca caatctttaa
     3901 cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat actaagtccc
     3961 tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt ctcgtaaaca
     4021 cgactaatac agaagactga aaggtcgtca gcctacgata atctccccat aaaatgtgac
     4081 atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtacaaat agggctatat
     4141 gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca aaataaagaa
     4201 caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc tctattatta
     4261 cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag cacttaaaat
     4321 tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga
     4381 agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc
     4441 tgctgctgaa gttgaagagc gcactcgtaa actgcagcaa tatcgcgaaa tgctgatcgc
     4501 tgacggtatt gacccgaacg agctgctgaa tagccttgcc gccgttaaat ctggcaccaa
     4561 agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa
     4621 aacctggact ggccagggcc gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg
     4681 taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca cttgcttaaa
     4741 atcccgccat cggcgggatt ttttattgtc cggtttaaga caatttaata agaataagct
     4801 ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat acggatgtta
     4861 cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa gggtgttatg
     4921 acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc agtcatggct
     4981 cttccctttc atatgatagg cttcaaccgt ttctttttcg attagcatat caattgcgtc
     5041 ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc
     5101 cgcactaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgcttttgg
     5161 tttttcaacc acgccaacca tcggtacgct ttcacccggc gctaattcaa cgcctttgca
     5221 atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct ggctatgacc
     5281 cgtttcatcg aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata
     5341 ttcatccaga ataacgtcag gcaaaataac agccacaggt tcatcaccca ctaccgggtg
     5401 agcacacaat accgcgtgtc ccaggccttt cgccagcccc tgacgaactt gcataatagt
     5461 tacgtgcggc ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc
     5521 cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt ttttagatga
     5581 gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg
     5641 aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa
     5701 catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc
     5761 agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca
     5821 tcccagtata tcagcacccc ggaggggcta agttctgaaa aggatgcgag ttagcataat
     5881 taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt agctggtaat
     5941 gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc gcagtcgacc
     6001 accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt gattcaaata
     6061 agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt
     6121 atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata
     6181 atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta aatggtttac
     6241 ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa gctgttcctg
     6301 taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc
     6361 tcgggttaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg caatatcaag
     6421 caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc aatgggaaat
     6481 cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg cagggttatc
     6541 aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat
     6601 gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt
     6661 cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcagt
     6721 ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct ccagcagttt
     6781 aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc
     6841 cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga
     6901 aaaccacgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga gaatctgttt
     6961 tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat agtggcatgc
     7021 tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt
     7081 tggtgcgtac caacggcaaa agttcgtcca ttttcttttc cactgccagc tgcccggccg
     7141 caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa
     7201 tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc tccagcacct
     7261 ggatagaagt ggtcataatc tctgttgccg ttggtgctgt caccgcccga cgtgtcgtta
     7321 tactgcctaa cctttctttc agacgcgcat gccacggcag agaatcacca ttctcgctat
     7381 tttcttcact gacattaaag gagagcaaat cttgttgcat caaatgagca tcgtgctgca
     7441 ggtcgaccgc tatcacaata tcagcaccca atgcacgcgt gagcgaaata ggaattgggt
     7501 taacgactgc cccatcaacc agccagtagc cgttatgcgc aacgggtgcc attagtcctg
     7561 gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac cataattcac
     7621 gcccagtact taaattggtg gcaacagccg cgaagcgacg ggaacaattt tcgatctctg
     7681 tttccggcat tatttcgcgg tattgattga agacacgctc gccgcgcagt aacccaccgc
     7741 gctgccagga gagatccatc agtcgtaaaa catcccaata actgaaagag gtcacccaat
     7801 cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa ccaattgaac
     7861 atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat
     7921 gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta
     7981 ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc ggttaacata
     8041 gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt gtgtctcagc
     8101 tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg
     8161 aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc
     8221 aagacgcaga ttatttaata aagacctggc atccatcttg tggagcagcg gcgttacgtg
     8281 ccgaattgat tgccggattt gcgcatactg aatggttggg attgaccgta tttgaacatt
     8341 gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt accgaaggtg
     8401 gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact
     8461 atattgacgg tacacgtccg cagtttggcc gcaacgatcc ctgcccttgt ggttcaggta
     8521 aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc
     8581 aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg
     8641 gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc
     8701 gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg
     8761 gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt gccagaaggc
     8821 tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagaa
     8881 gcgcattgcc ttggcgattt gttgatgaaa gccaattatg gcggcctgga tgtcgaaatc
     8941 gcggcagtga ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatataccg
     9001 tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa gatggcggat
     9061 gccattgatg cttatcaacc tgactacgtg gtgctggcga agtatatgcg ggtattaaca
     9121 ccggaatttg tgtcacgctt cccgaataag atcatcaata ttcaccattc cttcctgcca
     9181 gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggcgtgaa gattattggc
     9241 gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat gcaggacgtt
     9301 attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag
     9361 aaaaacgtct taagtcgcgc gctctacaaa gtactggcgc agcgcgtctt tgtttacggt
     9421 aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg
     9481 gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat
     9541 ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc attaccccgt
     9601 ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa
     9661 ggttcgaatc cttcccccac caccatgatt caccacagcg atgtggtaac ttcggtgaga
     9721 ttgtgaaagt ttgcactcat cggggaaggg tgagaacctt cgactaaggt tcgactcgag
     9781 cgaaagcgag agaacgtttt cacaggcaac ggcccgaagg gtgaggagcg aagcgacgaa
     9841 taatcctttc cccaccgcca tctcaagcat tacctcaaaa tttgaattac ccctggtggg
     9901 gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt cgaaggttcg
     9961 aatccttccc ccaccaccat cactttcaaa agtccctgaa ctctcaagcg aatccgtaaa
    10021 taaatattct gcccaagcgg agaaggatga gaagcttcga ctaaggttcg actcgagcgc
    10081 cagcgagaga gcgttgccgc aggcaacgac ctgaagggcg aagcgcgcag cgctgagtaa
    10141 tccttccccc accaccatca ctttcaaaag tccctgaact ctcaaacgaa tccgtaatta
    10201 aatattctgc ccaagcgggg aaggatgaga agcttcgacc aaggttcgac ttgagcgcca
    10261 gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa gcgcgcagcg ctgagtaatc
    10321 cttcccccac caccatcgct tcctgaaata atcaaaacct gttactccga gctaattttg
    10381 cccaatggca ccacgtttac cggacctaca atatcctccc ttgtaggtcg gataagcgaa
    10441 gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa accccgccga agcagggtct
    10501 atgtgaacta aaattcgctt agtgacgagc gcgcaccagc tggtacttac gtgtcagata
    10561 ctccaccggt acgctccaga tgtgtaccag acgcgagaac gggaacagca ggaataacgt
    10621 catccccagc accaggtgta gacggaagat aaacgccaca ccatcgaggt gttgagaagc
    10681 gccaccgtgg aaggtcacta ccgactgcgc ccagccaacc agtttcatca tctcgctacc
    10741 gtccatatgt tgggcggaga acggaatggt caacaggccc agcgcgcact ggataacgag
    10801 cagcgacagg atcagtatat ccgctccggt agtcgttgca cgcacgcgtg ggctgaacag
    10861 acgacgttta agcaaaagca cgccgccaat cagacacagc acgccgctgg caccaccagc
    10921 aaacatcgcc attttctgtt tcacttcaat cggcagccag gcttcataca tccagtgcgg
    10981 cgtcaacata ccgaagaagt gaccgacaaa aatccccaga ataccgatat ggaacaggtt
    11041 cgacgccagg ttcatccctt tgcgatccag catctggctg gacgccgcgc gccaggtgta
    11101 ctgcccgtag tcataacgca gccagctacc aatcaggaag accgccccgg ctatgtacgg
    11161 gtagatatca aagaagaaca tattcaggaa ttgcattagt gctgtcctcc ggtggtgata
    11221 ttcagatatt gcggcgcgac ggcaccggca aagcgacgct gatgagcagt gattgctgaa
    11281 tcaccgcagc ctttgtcagc aaagaattta acctgctctt cttcccaaac agcatccagc
    11341 gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt ccgccacttt gtcgctgtcg
    11401 atagcggtat tggccaattt cagcagcaga tcaaacagca cggcataacg gctttcacgc
    11461 tgttgcagac gcgcactcag caatgccaga atcggcgcga tatctttcaa accttccacg
    11521 gcttcgcttt gcggcagctg cgccaggtac tccagataca gcggcagatg atccggcaat
    11581 tcgcggctgt ttaactgcaa gccgtgctgc tcgtactgcg ccagcaggtc caccatcgcc
    11641 tggccgcggt cgcgggattc gccgtgcaca tgttcaaaca gcaacagtga cgtggcgcgg
    11701 ccacggtcga acagttcgct gtactgcgcc tgggcatcga gcggatccat cgtcgttaaa
    11761 tcgcgcagga aaatgcccag cgcatgggca tcctcttttg gcagattttt cgacgcggca
    11821 atcgcctcaa acatctcttg ttgatgctgc cataaggcag catccggata ttcaaggaga
    11881 cgcgatacaa tcacgagttc gatcatggat gcggctccgt tttgctggtc acatcgatgg
    11941 catcgatacg acggctgttg aacagattga atttggtatc tgaaccgtgg cagccatcac
    12001 caaaggtaaa gccgcagcca tttttctccg ggaaggcttc ccgtgccagt tcacgatgac
    12061 tactcggcac cacaaagcga tcttcgtagt tagcaatcgc cagataacgg tacatctcct
    12121 gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg ggtatcaact ttaccgtcaa
    12181 cggtttcagc acgtttgtaa tgacgcatcg ccagcatacg tttcagcgcg cgcagtaccg
    12241 gtttggtatc accggcggtc agcagattcg ccagatactg taccggaata cgcaggcttt
    12301 cgacgtctgg cagaatgccg ttgctaccca gctcacccgc gtctgctgca gactgaatcg
    12361 gagagagagg cggcacgtac cagaccatcg gcagtgtgcg atattctgga tgcagcggca
    12421 gcgccagttt ccattccatt gccattttat aaaccggcga ctgctgtgca gcttcaataa
    12481 cgctcagcgg aataccgtct ttaatcgcct gctcgatgac tttcggatcg ttcggatcga
    12541 ggaacacctc cagctgacgc tggtagagat ctttctcgtt ctcggtgctg gctgcacgtt
    12601 caatcgcgtc ggcatcgtac agcagcacgc caagataacg gatacgaccg acacaggttt
    12661 ctgagcacac ggtcggctga cccgcttcaa tacgcggata gcagaagatg cacttctcag
    12721 acttaccgct cttccagttg aagtagattt ttttgtacgg gcatccggtg atgcacatac
    12781 gccagccacg gcatttatcc tggtcgatca ggacgatgcc atcttcttca cgcttgtaaa
    12841 tcgcaccgct cgggcaggtc gccacacatg ccgggttcag gcagtgttcg cacaggcgcg
    12901 gcaaatacat catgaaggtg ttttcgaact ggctatacat cgccttctgg atgttgtcga
    12961 agttcttgtc tttcgccagt ttgtcaaact caccgcccag gtcatcttcc cagttcggcc
    13021 ctttttcgat tttcgccatc cgttccccgg taatcagcga acgcggacgg gcaatcggct
    13081 gcgatttgct gccttccggc gcggtatgca ggttctgata gtcaaaatcg aacggctcgt
    13141 aataatcgtc gatccccggc agatgcgggt tagcgaagat tttacccagc agcatggcac
    13201 ggttacccat gcgcggttgc agtttgccgt tgattttacg gatccagccg cctttgtatt
    13261 tttcctggtt ttcccagtca gtcgggaagc cctggcccgg cttggtttcc acgttgttga
    13321 accacgcgta ttccacgcct tcacggctgg tccagacgtt tttacaggta actgaacagg
    13381 tgtggcagcc gatgcactta tcgagattca gcaccatgcc gacttgtgaa cgaattttca
    13441 ttttacgctc tcctgtacct gatcattgcc ttcgccatct aaccagtcaa tgttcttcat
    13501 cttacgcact acaacaaact catcgcggtt agaacctacg gtgccgtagt agttaaagcc
    13561 gtatgccaga tgggcatagc cgccgatcat atgcgtcggt ttcggcgtga tacgggtgac
    13621 cgagttatgg ataccaccac gctgttgggt aatttccgaa ccaggcaggt taacgatacg
    13681 ttcctgcgcg tggtacatca tggtcatccc tgccggaaca cgctggctga caaccgcacg
    13741 ggcagtcaga gcaccgttgc tgttgaatac ttcaatccag tcgttatcgg cgatacccag
    13801 atctttggca tcggcttcac tcaaccagac caccggacca ccgcgaccta aagtcagcat
    13861 cagcaggttg tcgctgtagg tggagtggat accccacttc tggtgcggcg tcaggaagtt
    13921 gagcgctttt tccgggttgc cgttggattt ctggcctatc acttctttca ccgaacgggt
    13981 gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa tcacgcatcc actggtgatc
    14041 ctgatacagt tgctgacgac cagagagcgt acgccatggg atcagctcgt gaacgttggt
    14101 gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca gaccaggtcg ggctggagat
    14161 aattttgcgc ggctgtgcct gaatatcgcg gaagcggatc ttctcgtctt ctttattcag
    14221 cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg gcagcccagg ctttcacggc
    14281 tacctgaccg ttggtttccg gtgccagtgt caggatcatc tctgccgcat caattgcggt
    14341 gttcagcatc ggctggcctt tcgccggacc ttccgctttg gtgtagttga gcttacgcag
    14401 cagatccatc tcgctctggg tgttccaggc aatcccttta ccgccgttac cgattttctc
    14461 catcagcggg ccgatagagg taaagcgttc gtaagttgcc ggataatcgc gctctacgac
    14521 cataatgtgc ggtgcagttt tgcctgggat caggtcgcat tcgccttttt tccagtcttt
    14581 cacatccagc ggctgcgcca gttcagcggc agagtcgtgc tggataggca gcgtgacgat
    14641 gtcggtttct ttacccagat ggccgacgca cacttcggag aatttcttcg cgatggcttt
    14701 gtagatttcc cagtcgcttt tcgcttccca ggccggatcg accgccgcag acagcgggtg
    14761 aataaacgga tgcatatccg aagtattcat gtcgtctttt tcgtaccagg tcgccgtcgg
    14821 caaaatgatg tcggaataga gacaggtgct cgacagacgg aagtccagcg taaccaccag
    14881 atccagcttg ccttccagac cattgtcctg ccagtccact tcttccggct tcacgccgcc
    14941 ctgctgcccc agatctttac cctggatacc gtgctccgtc cccagcaggt acttgagcat
    15001 aaactcatga cctttaccgg aagaaccgag caggttagaa cgccagataa acaggttacg
    15061 cgggtggttt ttgccgtttt ccggctgttc cgcagcgaaa cggatagaac cctctttcag
    15121 ggatttcacc gtatagtcca ccggattcat cccggctttt tctgcttcgc cagcgatagt
    15181 cagcgggtta gtgcctaact gcggcgcaga cggcagccag cccatgcgtt ctgcacggac
    15241 gttaaagtcg atcagatgtc cggtatagcg ggatttatcc gccatcggcg acagcagttc
    15301 ttccgcagtc acggtttcat aacgccactg gctggagtgg ttatagaaat aagaggtgct
    15361 gttcatgtga cgcgccggac gctgccagtc gagggcaaac gccagcggct gccagccggt
    15421 ttgcggacgc agtttttcct gacctacata gtgcgcccag ccgcccccgc tctgaccgac
    15481 acagccgcag aaaatcagca tgttgatcag accacgatag ttcatatcga ggtgatacca
    15541 gtggttcagc cccgcaccga cgataatcat cgaacgaccg tgcgttttat cagcgttatc
    15601 ggcaaattca cgggcgatgc gaataatctg gctgcgagaa acgccggtaa tctgctcggc
    15661 ccaggccggg gtataagctt tcacatcgtc atagctggtt gcacagttaa cgtcgttcag
    15721 gccacgttcc agaccgtagt ttgccagcgt cagatcataa acggtggtca ccagggcggt
    15781 gctgccatca gccagttgca ggcgtttcac cggcagtttg tgcagcagca cgttttccag
    15841 ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag tacgggaagc ctacctcagc
    15901 gatctcatcc tggctaccca gcaggctcag ttgcagctcg gtttcttcgc cagttttgcc
    15961 gtcgcgctgc tcaagattcc atttgccctt ctcgccccag cggaagccaa tagaaccgtt
    16021 cggcgcaacc atttcgccat tggtattaaa ggcgacagtt ttccattccg gattgttttc
    16081 ctggcccagc gcatcaacca gatcagcagc gcgcagcata cgacctgcag cgtagtaacc
    16141 gtcgcgttct tccagcatca ccagcatcgg catgtcggtg tagcgacgca catagtcggt
    16201 gaaatactgg cttgggttgt cgagatggaa ttcacgcagc attacgtggc ccatcgccag
    16261 cgccattgcc gcatcggtgc cctgtttcgg tgccagccac agatcgcaca gtttggcgat
    16321 ttcagcgtag tctggtgtga cggcaacagt tttggtgcct ttgtaacgca cttcagtaaa
    16381 gaagtgagca tctggagtac gcgtctgcgg cacgtttgaa ccccaggcga tgatgtagct
    16441 ggagttgtac cagtcagcag attccggtac gtcagtttgc tcgccccagg tttgcggaga
    16501 cgcaggaggc aggtcgcagt accagtcgta gaagcttaag caagtaccgc caatcagcga
    16561 gagatagcgt gcacccgatg cgtaagaaac catcgacatt gccggaattg gcgagaaacc
    16621 agcaacacgg tccgggccgt agtttttgat ggtgtaaacg ttagatgcgg cgatcagttc
    16681 gttcacctcc tgccaggaag aacgaacaaa accaccgcgt ccacgcgcct gcttaaagct
    16741 tttcgcttta tcggcgtctt caatgataga agcccatgcc tcaaccggat cgctatgcag
    16801 cgccttcgct tcacgccaca ttttcatcag gcgtttgcgc atcatcgggt atttcaggcg
    16861 gttggcactg taaagatacc aggagtagct ggcaccgcgc gggcagccgc gaggttcatg
    16921 gtttggcaga tccggacggg tacgcggata gtcagtctgc tgggtttccc aggtgaccag
    16981 accgtttttg acgtagattt tccagctgca ggagccggtg cagtttaccc cgtgggtaga
    17041 gcggacgatt ttgtcatgct gccaacgctg gcgatatcca tcctcccagt cacggttggt
    17101 attgagaagc tggccatgcc catcggcaaa ggtttcaccc ttctgtttga agtagcgaaa
    17161 ccggtccagg aatttactca tcggttttct cctgtgggag cctgtcggcc ctctgataaa
    17221 tcgacattgc taatgtttaa gggcaaggta acgctctgaa acagcg
//
LOCUS       DBFIFS010000084        17114 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.084, whole genome shotgun sequence.
ACCESSION   DBFIFS010000084 DBFIFS010000000
VERSION     DBFIFS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.084"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            138..1481
                     /locus_tag="N7A07_002490"
     CDS             138..1481
                     /locus_tag="N7A07_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477132.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1630..3138)
                     /gene="putP"
                     /locus_tag="N7A07_002491"
     CDS             complement(1630..3138)
                     /gene="putP"
                     /locus_tag="N7A07_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU2477133.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3297..3506)
                     /locus_tag="N7A07_002492"
     CDS             complement(3297..3506)
                     /locus_tag="N7A07_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477134.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3561..7523
                     /gene="putA"
                     /locus_tag="N7A07_002493"
     CDS             3561..7523
                     /gene="putA"
                     /locus_tag="N7A07_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU2477135.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7563..8201)
                     /gene="rutR"
                     /locus_tag="N7A07_002494"
     CDS             complement(7563..8201)
                     /gene="rutR"
                     /locus_tag="N7A07_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU2477136.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8489..9580
                     /gene="rutA"
                     /locus_tag="N7A07_002495"
     CDS             8489..9580
                     /gene="rutA"
                     /locus_tag="N7A07_002495"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU2477137.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9580..10272
                     /gene="rutB"
                     /locus_tag="N7A07_002496"
     CDS             9580..10272
                     /gene="rutB"
                     /locus_tag="N7A07_002496"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU2477138.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10284..10623
                     /gene="rutC"
                     /locus_tag="N7A07_002497"
                     /pseudo
     CDS             10284..10623
                     /gene="rutC"
                     /locus_tag="N7A07_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
     gene            10631..11431
                     /gene="rutD"
                     /locus_tag="N7A07_002498"
     CDS             10631..11431
                     /gene="rutD"
                     /locus_tag="N7A07_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU2477139.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11441..12031
                     /gene="rutE"
                     /locus_tag="N7A07_002499"
     CDS             11441..12031
                     /gene="rutE"
                     /locus_tag="N7A07_002499"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU2477140.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12042..12536
                     /gene="rutF"
                     /locus_tag="N7A07_002500"
     CDS             12042..12536
                     /gene="rutF"
                     /locus_tag="N7A07_002500"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU2477141.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12557..13885
                     /gene="rutG"
                     /locus_tag="N7A07_002501"
     CDS             12557..13885
                     /gene="rutG"
                     /locus_tag="N7A07_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU2477142.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(13968..14075)
                     /locus_tag="N7A07_002502"
     CDS             complement(13968..14075)
                     /locus_tag="N7A07_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HGU2477143.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            14447..15043
                     /gene="wrbA"
                     /locus_tag="N7A07_002503"
     CDS             14447..15043
                     /gene="wrbA"
                     /locus_tag="N7A07_002503"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU2477144.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15064..15291
                     /gene="yccJ"
                     /locus_tag="N7A07_002504"
     CDS             15064..15291
                     /gene="yccJ"
                     /locus_tag="N7A07_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU2477145.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15329..16570)
                     /gene="agp"
                     /locus_tag="N7A07_002505"
     CDS             complement(15329..16570)
                     /gene="agp"
                     /locus_tag="N7A07_002505"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU2477146.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(16861..>17114)
                     /locus_tag="N7A07_002506"
     CDS             complement(16861..>17114)
                     /locus_tag="N7A07_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477147.1"
                     /translation="EVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYL
                     INTGNHYDVYLPPELFAQAYELNNKERNAQIGFLTRYAI"
BASE COUNT         4070 a         4551 c         4526 g         3967 t
ORIGIN      
        1 aatgagcgga ttgtttacta catcaagaac gcatccgaag tgtacatcct ggtgcgccga
       61 ctgaatcgcc cggagccatg cggatggcta cgacttaata cgaatcatta accctgtaag
      121 gatacggaga gcattacatg agaccaatat ccaacctcaa tacgagcgca gcaacctata
      181 ttccaccgca acaactcccg agtagttgtc tggaaacttt gacgcttctg gtgcagatga
      241 ataattatat caaaagggat gcggactaca gcacgggcat ggctgtagct ccactggttc
      301 ctgaagaggt tcacttgctg gctgctgcga tgacaaccga actgcatcga catcagttcc
      361 aaccggtggt aagtgcaaat gatctgcaac ttcctgaacc gttcaccttc gatgttgccg
      421 gtttcaccat caccttcacg aagacgcaag agcatgacaa cacgggcaat ctcatgaaaa
      481 ttgcagtcag caagagcggt gtgtgcacca gtacaaatat tactcttgag ctgtttcact
      541 ccattgtcac aaccctgatg tcacgcagtc aatatggaac ctttgatctc tggtccgtac
      601 ggccaatact cacggatgaa agtcaaaaca gggtgcatga agcagcccgc tactccccgg
      661 cccagcaata cggaagagaa gaaacacact ttaccaacaa tgggatgcga gaatttggaa
      721 atatgtctcc gctggcgtgg cgtaacgatg tggaattaca gcaatcgtgt aatacaagca
      781 acatcccacc aaatgctgca aatgacgaca taaacaacac caggcagacc atcgaggacc
      841 agccagaggc ggacgagcca cagcaatatg ttaaattgac tgtagacgac atgcgaaaat
      901 gggcggctat ggaccagcag gcgcgaaacg ctctacaggg agtttcagga tggtgtacgc
      961 gcaatcattt taatatcaaa aatgccagaa attacctgac cgatcacggc ctgaactacg
     1021 cgggacaggt aaaggtaaac aggccgcatg aatatgccaa attcaccctt gaacatatcc
     1081 gccagtgggc agccttaaac aagcatgtgc gaaaaccggt tggttattta gaaaagtggt
     1141 gtaaggaaag aaacctcgcg ccaactactg ccaggaatta cctgaagaac gacgggctta
     1201 ccgccctggg tgagttgaag ctgacagggc cgcagaaatg ggtcacgttc accttcggag
     1261 acatcgtaca atgggcgaat atgacccagg aggagcgtaa cagcgcaggg ggagccgaaa
     1321 agtggagtaa aaaacgcggc ttccagtggt ctactgccag atcctacctg aaatcgagcg
     1381 gagtgaccaa gcaaggtgcg agaaaactgg cgtggttaaa aaacagcggg aatatgtcga
     1441 atccattcta tctcgcccaa ccaaccagcc gcaggacata agctgaaggt accgatagcg
     1501 gcaattttgt tcatctgtgc cgggtgggca aatagcaatt gcccccgaac actccctgcc
     1561 aggcaactta taccgcatgg cagggaacat tcgggttaaa aagcaaaaga taactaatta
     1621 ctcttcagct tagccttcct gcaaccgtga cggcggagcc gaatgatagt gcgcatcggc
     1681 ctcggcaaag cgtttttgca tcgccgctga cggtgcttta cccagcaaac taaacactac
     1741 aatcccaata ctgccaaaga taaagcccgg aataatttcg tacagtccca gccagccgaa
     1801 ctgtttccag acgatcaccg tcagcgcacc gatgatcatc cccgccagcg caccgttgcg
     1861 cgtcatgcgt gaccacatca ccgaaaacag caccaccgga ccaaacgccg cgccaaagcc
     1921 tgcccacgcg tagctcacta agcccagcac gcggttttcc gggttcgctg ccaacgcaat
     1981 cgccaccagc gccaccacca gcaccatcac acgccctacc cacaccagtt ctttctggct
     2041 ggcatgttta cgcagaaacg ctttgtacaa atcttcggta atcgcactgg aacataccag
     2101 cagctggcaa cttaacgttg acattaccgc cgccagaatc gccgacagca gaatcccggc
     2161 aatccacggg ttaaacagaa tttgcgccag ttcgataaac acgcgctcgg cgttctggtt
     2221 taccgcacca gctaccgacg ggtgctcgtt aaagtaagcg ataccaaaga agccgacagc
     2281 caccgcccct gccaggcaga ggatcatcca ggtcatactg atacgacgcg catggacgat
     2341 gctgtggtga gaatccgccg ccataaaacg cgccaggatg tgcggttgcc cgaagtaacc
     2401 cagcccccac cccatcagcg agataatggc gacaaagttc agccctttga gcatatccac
     2461 gttttcgatg ctcttttgtt tgatcacttc cagcgagtca ccaaagccac cgacactgat
     2521 aatgacgata accggcgtca ggatcagggc aaaaatcatc aggctggcct gtacagtgtc
     2581 agtccagctc accgcgagga aaccgccaat aaaggtgtaa aggatcgtcg ccgcagctcc
     2641 cgcccacaga gccgtttcgt agctcatgcc aaaggtactt tcaaacagac gcgcgcctgc
     2701 cacaatgccc gaagcgcaat aaatggtgaa gaacagcaaa atcaccagcg cggagataat
     2761 gcgcaaaatg cggcttttat cttcaaaacg cccggtgaaa taatccggta atgttaaggc
     2821 gttattgttg tattcggtat gcacacgcaa ccgcccggcc accagcttcc agttaatcca
     2881 cgcgcctaat gtcaggccaa tggcgatcca actttcggaa atcccggaaa gaaaaaccgc
     2941 gcccggcaac cccattaaca gccagccgct catatccgat gcacccgccg ataatgccgt
     3001 cacgaatggc ccaaggctac gtccgcccag aatatagtcg tcaaagtttt tcgttgatcg
     3061 ccaggcgata aacccaatca atatcatgcc aaagatatag acacaaaatg tcaccaacat
     3121 cggtgtgcta atagccatct aaagtctcca aaaaattatt atcggcaatg tcgaaacttg
     3181 ccgttatatc tgccaccgga acggggtaac agagtttatg ttttaccagg gcgaccgtat
     3241 cctgccggaa gcgctggtta ttcacaatcg atttaacaca ccatttacat taaattttag
     3301 tgctcagcga cactattttt catcaggttg cactctctca cattttttgc ggttgcacct
     3361 ttcaaaaatg ttaactgccg cagagaaaaa gtctgagtta tttttttaat ccctgtcata
     3421 tcgatttctt ttattaacat ttcattcatt tttaagcttg ctacgcaggt cacatttaac
     3481 atggttgcac aaagttgcaa catcatggat atttcacgat aacgttaagt tgcacctttc
     3541 agaacaacag gagtaatggc atgggaacca ccaccatggg ggttaagctg gacgacgcga
     3601 cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg cacgccacac tggttaatta
     3661 agcaggcgat tttttcttat ctcgaacaac tggaaaacag cgatactctg ccagagctac
     3721 ctgcgctgct ttctggcgct gccaatgaga gcgatgaagc accgactccg gcagaggaac
     3781 cacaccagcc attcctcgac tttgccgagc aaattttgcc ccagtcggtt tcccgcgctg
     3841 cgatcaccgc ggcctatcgc cgcccggaaa ccgaagcggt ttctatgctg ctggaacaag
     3901 cccgcctgcc gcaaccagtt gctgaacagg cacacaaact ggcgtatcaa ctggccgata
     3961 aactgcgtaa tcaaaaaaat gccagtggcc gcgcaggtat ggtccagggg ttattgcagg
     4021 agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg tctggcagaa gcgttgttgc
     4081 gtattcccga caaagccacc cgtgatgcgt taattcgcga caaaatcagc aacggtaact
     4141 ggcagtcaca cattggtcgt agcccgtcac tgtttgttaa tgccgctacc tgggggctgt
     4201 tgtttactgg caaactggtt tccacccata acgaagccag cctctcccgc tcgctgaacc
     4261 gcattatcgg taagagcggt gaaccgctga tccgcaaagg tgtggatatg gcaatgcgcc
     4321 tgatgggcga gcagttcgtc acgggcgaaa ccatcgcgga agcgttagcc aatgcccgca
     4381 agctggaaga gaaaggtttc cgttactctt acgatatgct cggcgaagct gcactgactg
     4441 ccgcagacgc acaggcgtac atggtttcct atcagcaggc gattcacgcc attggtaaag
     4501 cgtctaacgg tcgtggcatc tatgaaggtc cgggcatttc aatcaagctg tcggcgctgc
     4561 atccgcgtta cagccgcgcc cagtatgacc gggtaatgga agagctttac ccgcgtctga
     4621 aatcactcac cctgctggcg cgtcagtacg atattggtat caacattgac gccgaagagg
     4681 ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact ctgtttcgag ccagagctgg
     4741 caggctggaa cggcatcggt tttgttattc aggcttatca aaaacgctgc ccgctggtga
     4801 ttgattacct gattgatctc gccacccgca gccgtcgccg tcttatgatt cgcctggtga
     4861 aaggcgcgta ctgggatagt gaaatcaagc gcgcgcagat ggacggcctt gaaggttatc
     4921 cggtttatac ccgcaaggta tataccgacg tttcttatct cgcctgtgcg aaaaagctgc
     4981 tggcggtgcc gaatctaatc tacccgcagt tcgcaacgca caacgcccac acgctggcgg
     5041 cgatttatca actggcaggt cagaactact atccgggtca gtacgagttc cagtgcctgc
     5101 atggtatggg cgagccgctg tatgagcagg tcaccgggaa agttgccgac ggcaaactta
     5161 accgtccgtg tcgtatttat gccccggtcg gcacacatga aacgctgctg gcgtatctgg
     5221 tgcgccgcct gctggaaaac ggtgctaaca cctcgtttgt taaccgtatt gccgatacct
     5281 ctttgccact ggatgaactg gtcgccgatc cggtcactgc tgtagaaaaa ctggcacaac
     5341 aggaagggca aactggatta ccgcatccga aaattcccct gccgcgcgat ctttacggtc
     5401 acgggcgcga caactcggca gggctggatc tcgctaacga acaccgtctg gcatccctct
     5461 cttctgccct gctcaatagt gcactgcaaa aatggcaggc cttgccaatg ctggaacaac
     5521 cggtagcggc aggtgagatg tcgcccgtta ttaacccggc ggaaccgaaa gatattgtgg
     5581 gctatgtacg tgaagccacg ccgcgtgaag tagagcaggc gctggaaagt gcggttaata
     5641 acgcgccaat ctggtttgcc actcctccgg ctgaacgcgc ggcgattttg catcgcgctg
     5701 ccgtgctgat ggaaagccag atgcagcaac tgattggtat tctggtgcgt gaagccggaa
     5761 aaaccttcag taacgccatt gccgaagtgc gtgaagcggt cgattttctc cactactacg
     5821 ccggacaggt gcgggatgat ttcgctaacg aaacccatcg tccattaggg cctgtggtat
     5881 gtatcagccc gtggaacttc ccgctggcga ttttcaccgg gcagatcgct gccgcactgg
     5941 cggcaggtaa cagcgtactg gcaaaaccgg cagaacaaac gccactgatt gccgcgcaag
     6001 ggatcgccat tttgctggaa gcgggcgtac cgccaggcgt ggtgcaattg ctgccaggtc
     6061 ggggtgaaac cgtgggcgcg caactgacgg gtgatgatcg cgtacgcggg gtgatgttta
     6121 ccggttcaac cgaagtcgct acgttactgc agcgcaatat cgccagccgc ctcgacgctc
     6181 agggtcgccc tattccgctc atcgctgaaa ccggcggcat gaacgcgatg attgtcgatt
     6241 cttcagcact gaccgaacaa gtcgtaatag atgtgctggc ctcggcgttc gacagtgcgg
     6301 gtcagcgttg ttcggcgctg cgcgtgctgt gcctgcaaga tgagattgcc gaccacacgt
     6361 tgaaaatgct gcgcggcgca atggccgaat gccggatggg taatccgggt cgcctgacca
     6421 ccgatatcgg tccggtgatt gatagcgaag cgaaagccaa tattgagcgc catattcaga
     6481 ccatgcgtag caaaggccgt ccggtgttcc aggcggtgcg ggaaaacagc gaagatgccc
     6541 gtgaatggca aagcggcacc tttgtcgccc cgacgctgat cgaactggat gactttgccg
     6601 aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt gcgttataac cgtaatcagc
     6661 tgccggagct gatcgagcag attaacgctt ccggctatgg tctgacgctt ggcgttcata
     6721 cgcgtattga tgaaaccatc gcccaggtca ctggctcagc ccatgttggt aacctgtatg
     6781 ttaaccgtaa tatggtgggc gcagtggttg gggtgcagcc attcggcggc gaagggttgt
     6841 ccggtaccgg gccgaaagca ggcggtccgc tctatctcta ccgtctgctg gcgaatcgcc
     6901 cggaaagtgc gctggcggtg acactcgcgc gtcaggatgc agagtatccg gtcgatgcgc
     6961 agttgaaagc cgcattgact cagccgctaa atgcactgcg ggaatgggca gcaaatcgtc
     7021 cagaattgca ggcgttatgt acgcaatatg gcgagctggc gcaggcagga acacaacgat
     7081 tgctgccagg gccgacgggt gagcgcaaca cctggacgct gctgccgcgt gagcgcgtat
     7141 tgtgtatcgc ggatgatgaa caggatgcgc tgactcagct cgccgccgtg ctggcggtgg
     7201 gcagccaggt gctgtggcca gatgacgcgc tgcatcgtca gttagtgaag gcattgccat
     7261 cgactgtcag cgagcgtatt caactggcga aagcggaaaa tattaccgct caaccgtttg
     7321 atgcggtgat cttccacggt gattcggatc agcttcgcgc attgtgtgaa gcagttgccg
     7381 cgcgggatgg cgcaattgtt tcggtgcagg gttttgcccg tggcgaaagc aatatcctgc
     7441 tggaacggct gtatatcgag cgttcgctga gtgtgaatac cgctgccgct ggcggtaacg
     7501 ccagcttaat gacgatcggt taaacaggta gccggaggat gttacaccct cctccggcat
     7561 cattagcgtg gtcgaatccc ctcaataata atccgctgca cgttttcaac cgtttgattg
     7621 aaaaatacct catcgcgcaa cgttgcgcct gtcaccgcct ccacctgagg ggcgaaatcg
     7681 gcgtaatgtt gagtggaagc ccaaatcata aaaatcaaat gctgcggatc aatcggcgcg
     7741 agtttgccgc ttttgaccca accggcaatc agcgccgatt tctcatcaat taatgacttc
     7801 aaatcgcccg tcagttcatc cattaacagc ggcgcgcctg ccagcatctc catacagaac
     7861 aggcgcgaag cctgcggata atcgcgtgag acttccagct tcagacggat gtactctttg
     7921 atcgccgcca gcggggcgaa atcttcacga aacgctttta acggtgccag ccagatatcg
     7981 agaatctgcc gcagcacggc aatatacagc gcctcttttg acggaaagta atacagcaga
     8041 ttggtttttg aaacacccgc caactctgca atttgctcca gccgtgtgcc gtgaaaaccg
     8101 aattgtgaaa aagtgtccag tgctgcgcta agaatcgctt ttttcttcgc gcttactgcg
     8161 cgcgaacgtt tacccgttgt tttcactgcg ccttgcgtca tgcgctctcc cctcttgtag
     8221 attggcaaca ggatagcaaa acccccgccc ggcctcgacc ttctgcactc tcatcgcgca
     8281 cagatgcatg ttttatgtgc aactgttttg accgtttagt ccacttttta ccagacattt
     8341 tagataacgc gatgttaaca cattaataac actccctttt taaaaactgg catccgcttt
     8401 gcaaacaagc caatacgcgg ctgaaaagga tatgcagaat gcagcgcccc gtctgacttt
     8461 cactttacga gacgaagaga ggttgatgat gaaaattggc gtattcgtac ctattggcaa
     8521 caacggctgg ctcatttcga cccacgcgcc gcagtacatg ccgacctttg aactgaataa
     8581 agccatcgtg caaaaagcgg agcactacca tttcgatttc gccctgtcga tgatcaaact
     8641 gcgtggcttt ggcggcaaaa ctgagttctg ggatcacaac cttgagtcgt tcaccttgat
     8701 ggcggggctg gcggccgtga cctcgcgcat tcagatttac gccaccgccg ccaccttaac
     8761 gttacctccg gcaatcgtcg cccgtatggc cgcaaccatc gactccatct ctggcgggcg
     8821 ttttggcgtc aacctcgtga ctggctggca aaagcccgag tatgagcaga tgggtatctg
     8881 gcctggcgat gactatttct cccgtcgtta cgactatctc accgagtatg ttcaggtgct
     8941 gcgcgacctg tggggcacgg gaaaaagcga ttttaaaggc gattttttca ccatgaatga
     9001 ttgtcgcgtc agtccgcaac cgagtgtccc catgaaagtg atctgcgccg ggcaaagcga
     9061 cgctggcatg gcgttctccg ctcagtatgc cgatttcaac ttctgtttcg gcaaaggcgt
     9121 aaatacaccc acggctttcg ccccgaccgc tgcgcggatg aaacaggccg cagagcaaac
     9181 cgggcgcgac gttggctctt atgtattgtt tatggtgatt gccgatgaaa ccgacgatgc
     9241 cgctcgcgcc aaatgggaac actacaaagc gggcgcggat gaagaggcgt taagctggct
     9301 aaccgaacaa agtcagaaag atacccgctc cggtactgac accaacgtcc gtcagatggc
     9361 cgatcccact tcggcggtaa acatcaatat ggggacgtta gtcggttctt acgccagtgt
     9421 cgcgcgcatg ttagatgaag tcgcaagcgt gcctggtgcc gaaggcgtgc tgttaacctt
     9481 cgacgatttt ctgtcgggaa tcgaaacctt cggcgagcgc attcaaccac tgatgcagtg
     9541 ccgcgcccat ctccctgtgc tgactcagga ggtggcatga tgacgacatt aaccgctcga
     9601 ccggaagcca ttaccttcga tccgcagcaa agtgcgctga tcgtggtgga tatgcaaaac
     9661 gcctatgcca cgccaggcgg ctacttagat ctcgccgggt ttgatgtctc aaccactcgc
     9721 ccggtcattg ccaacattca aaccgccgtg accgcagcgc gagcggcagg gatgttgatc
     9781 atctggtttc aaaatggctg ggatgaacag tatgtcgagg ctggcggccc tggctcaccg
     9841 aattttcata aatcgaacgc cctcaaaacc atgcgtaaac agccgcagct gcaggggaaa
     9901 ttgctggcga aaggctcctg ggattatcaa ctggtggatg aactggtgcc gcagcctggc
     9961 gatattgtgc tgccgaagcc gcgctacagc ggtttcttca atacgccgct ggacagcatt
    10021 ttgcgcagcc gcggaatacg ccatctggtt ttcaccagca tcgctaccaa cgtctgcgtc
    10081 gaatcgacgc tacgcgatgg cttttttctg gagtatttcg gcgtggtgct ggaagacgca
    10141 acacaccagg cggggccgga atttgtacag aaagccgcgt tgttcaatat cgaaaccttt
    10201 tttggctggg tcagcgacgt cgaaacgttc tgcgacgcgc tttctcccac gtcctttgct
    10261 cgtatcgctt aaggagttta acgatgccaa aatccgtaat tattcccgct ggcagcagcg
    10321 caccgctggc ccccttcgtt cccggcacgc tggctgatgg cgtggtgtat gtctccggta
    10381 cgctggcttt tgatcaacac aataacgtgc tgtttgccga tgacccaaag gcgcaaaccc
    10441 gccacgttct ggaaactatc cgcaaggtaa tcgagacagc gggtggcacg atggcggatg
    10501 tgaccttcaa cagcatcttt attaccgact ggaaaaatta cggcgcgatt ctgcattcag
    10561 tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca tatcgccaag
    10621 tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatgcgc ccgtagtggt
    10681 gttgatttcg ggtcttggcg gtagcggcag ttactggtta ccgcaactgg cggtgctgga
    10741 gcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc ccgacacgct
    10801 ggcagaagat tacagtatca cccagatggc agcggaactg catcaggcgc tggtagccgc
    10861 agggattgag cattacgcag tggtcggcca tgcgctcggt gcgctggtgg gaatgcagct
    10921 ggcgctggat catcccgcgt cggtaactgt gctggtctgc gttaacggct ggctacgaat
    10981 aaacgcccat acgcgccgct gttttcaggt tcgcgaacga ttactgtata gcggtggcgc
    11041 gcaggcatgg gtggaagcgc agccgttgtt cctctatccc gccgactgga tggcggcccg
    11101 cgcacctcgc cttgaggcag aagacgcgct ggcactggcg cattttcagg gcaaaaataa
    11161 tttactgcgt cgacttaacg ccctcaaacg cgctgacttt agtcaccatg cggatcgcat
    11221 ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccat cagcatgttc
    11281 cagtgaactt catgccgccc tgcccgatag ccagaaaatg gtgatgcgct atggcggaca
    11341 cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg ttactcaacg ggcttgccag
    11401 cctgttacat caccgtgaag ccgccctgta aggaattgct atgaacgaag ccgttagccc
    11461 aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac aacggctggc gggagacacc
    11521 cgtcagcgat gagacgttac gggagcttta tgccctgatg aaatgggggc cgacatcagc
    11581 taactgttct ccggcacgta tcgtgtttat ccgcactgca gagggaaaag aacgtctgcg
    11641 cccggcgctt tccagcggca atctgcaaaa aaccctgacc gcgcccgtca ccgctatcgt
    11701 cgcatgggac agtgaatttt atgaacggtt accgcaactg tttcctcacg gcgatgcccg
    11761 cagttggttt acctccagcc cacaacttgc cgaagaaaca gcgtttcgca acagttccat
    11821 gcaggcggct tatctgatca tcgcctgccg tgcgctgggg ctggataccg gcccgatgtc
    11881 gggttttgat cgtcaacacg tagacgacgc tttttttgca ggcagcacgc tgaagagcaa
    11941 tctgctgatt aatatcggct atggcgatag cagcaaactt tttgcgcgcc tgccacgtct
    12001 gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac tatgaacatt gtcgatcaac
    12061 aaacttttcg cgatgcgatg tcctgcatgg gcgcggcggt caatatcatc accacggacg
    12121 gtccagccgg gcgcgccggg ttcaccgcca gcgccgtctg cagcgtgacc gacacgcctc
    12181 ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg gccggtattc aatgaaaatc
    12241 gaacgctgtg tgtaaataca cttagcgccg ggcaggagcc gctttcaaac ctttttggcg
    12301 gcaaaacgcc catggaacac cgctttgccg ccgcccgctg gcagaatggt gtgaccggat
    12361 gcccgcaact ggaagaggcg ctggtttcgt ttgactgccg tatcagccag gtggtgagcg
    12421 tcggcaccca cgacattttg ttttgcgcca tcgaagcgat tcatcgtcac gccaccccct
    12481 acgggctggt gtggttcgat cgcagttatc acgcgctgat gcgccctgct tgttaacctc
    12541 cttaaggaga cagctgatgg caatgttcgg tcttcctcac tggcagttaa aatcgacctc
    12601 tacagagagc ggcgtggtcg cgccggatga acgattgccg tttgctcaga cggcagtaat
    12661 gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg ttaatgccga ttctgatggg
    12721 gctggatccc aatctttcca ttttaatgtc agggatcggc acgttactgt ttttctttat
    12781 taccggcggg cgcgtgccca gttatctcgg ctccagcgcg gcttttgtcg gcgtggtgat
    12841 cgccgcaacg ggctttaacg gtcagggcat taacccgaat atcagcattg cgctgggcgg
    12901 gattatcgcc tgtgggctgg tttataccgt cattggcctg gtggtgatga aaatcggtac
    12961 gcgctggatt gaacggctaa tgccgccggt ggtgacgggt gcggtggtga tggcgattgg
    13021 cctgaactta gcgcctatcg cggtaaaaag tgtatcggct tcaggctttg atagctggat
    13081 ggcggtaatg acggtgctgt gtattgggct ggtggcggtg tttacccgtg gcatgatcca
    13141 gcggttattg attcttgtag gattaattgt cgcctgtctg ctgtatggcg tgatgaccaa
    13201 cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc agccatgctg cctggttcgg
    13261 tctgccacat ttttccactc ctgcttttaa tggtcaggca atgatgctga ttgcgcccgt
    13321 ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag gcggtcgccg ggatgaccgg
    13381 acgcaatatg gacccgtata tggggcgagc attcgtaggc gatggtctgg caacgatgct
    13441 ttctggttct gtcggcagta gcggcgtcac cacctatgct gaaaatatcg gtgtgatggc
    13501 cgtgacaaaa gtctactcga cgctggtgtt tgttgctgct gccgtcatcg ccatgctgtt
    13561 gggcttttca ccgaaatttg gcgcgctgat tcataccatt ccggccgcgg tgattggtgg
    13621 tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc gcaaggatat gggtacaaaa
    13681 ccgtgtcgat ttaagccaga acggtaattt gattatggtc gcagtaacct tagtgctggg
    13741 cgcgggtgat tttgctctca cgctgggcgg ttttacgttg ggaggaatcg gtacagcaac
    13801 ctttggcgcg attttactca atgcgttatt aagcagaagg ttggttgacg ttccaccgcc
    13861 agaagttgtt catcaggagc cataaacaga aaaccgccgg ggttaccggc ggtgatggct
    13921 ttttgacgag cttaagaaaa aaatgcattc gtcattgatt agcccggcta gttgtcgctt
    13981 ttgccgtgac tgctcttgcc acctttttta cctgcttctg atgctctttc gcggtcttct
    14041 gcaaaattgc cggaaccgcc tcgatggttt gccattactg acctccgtct gggtatagac
    14101 atcatattgc tagaattaac gcggcgtatg gcggctgcca tattttcgct acgtgggact
    14161 aagcttagtg aactgacgtg tttctacccg cgtttggtaa tatcctgcaa cagcttctta
    14221 gcgagaatag atatcatctc caataattat ccataagccg ctcttatgat taaaaaaacg
    14281 cgatgagata aaaatgtgtc attttgcgac aaaattgcgt gcttgtgaaa gttgttataa
    14341 atcaaataag tggttgtgaa atttgcactc tgaaaaggac gtcttatctt taaataagtg
    14401 gtagcgaatc gctacggaat agagataaca cgaggagtgg ttagaaatgg ctaaagttct
    14461 ggtgctttat tattccatgt acggacatat tgaaacgatg gcacgcgcag tcgctgaggg
    14521 tgcaagcaaa gtggatggcg ctgaagttgt cgttaagcgt gtaccggaaa ccatgtcgcc
    14581 gcaattattt gaaaaagcag gcggtaaaac gcaaactgca ccggttgcaa ccccgcaaga
    14641 actggccaat tacgacgcca ttatttttgg tacacctacc cgctttggca acatgtccgg
    14701 tcaaatgcgt accttcctcg accagacggg cggcctgtgg gcttccggcg cactatacgg
    14761 aaaactggcg agcgtcttta gttccaccgg tactggcggc ggtcaggaac aaaccatcac
    14821 atccacctgg acgacccttg cgcatcacgg catggtgatt gtccccattg gctacgcagc
    14881 gcaggaatta tttgacgttt cacaggttcg cggcggtacg ccgtacggcg caaccaccat
    14941 cgcaggcggt gacggttcac gccagcccag ccaggaagaa ctgtctattg ctcgttatca
    15001 aggggaatat gtcgcaggtc tggcagttaa acttaatggc taatcttcaa caggaggata
    15061 cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg aatgggcatc tctgcgcaat
    15121 acgtcgccgg aaatagccga ggccattttc gaagtcgccg ggtacgacga aaaaatggcg
    15181 gaaaagattt gggaagaagg tagcgatgaa gtgttagtca aagcctttgc taagaccgac
    15241 aaagattcgc ttttttgggg cgaacagacc atcgaacgta aaaacgttta accagcgact
    15301 cccccgtagc gaacggggga gttttctgtt atttcaccgc ttcattcaac acgctatcaa
    15361 acttatccat cgggcagaaa ccatcagcgt ctatcgggca accgcttaat tccagcgtca
    15421 cgcgctgcgc aggtgcctgc agggttaacg catcggcatt acgtaactgt tccgcactct
    15481 ggtacacata ttcaattttc atcaaatcgc gattggcttt gctgtcacgc caacgctgga
    15541 aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc atgcaactga tacggtttga
    15601 aatccagcgc cgttaacaga gaggcaatgt tggagtcatg cccaactaac accgtgattt
    15661 ttggagcact ggtgcgatcg gtgaccaggg ctttgtcgat ataactgacc agcggtttcg
    15721 caacattgcg cgccacttcc ggtgaggtaa acaggctgtc ctggtaaccg tttttcagct
    15781 tcgacaacac cttccactgc tggtcagatt tgatttctcc ccaggccacc tgatccatcg
    15841 gaaaaccttc gtagtattgc aaagtaaacg catctaccag cgagttgccg actttcagcg
    15901 gcccggaaac acctggttct tgttgatact tggcgctaaa ggtatttttg ccatccacca
    15961 gcgaacactg ttgtttctct ttacaggcag gggaatcttt atagttaacg attttttcca
    16021 gtagctggta gctgtcggta agctggagtt tgctgagctc tttctccatt gccgccaccg
    16081 cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg gttaaaggtt gggtccatag
    16141 tgcccatttt ttcctggtga tgcacaggaa tatcacaccc cgggaatgcg ccggtaataa
    16201 agaactgtgc ggtcgcaacg gtacgttgca gactattggc ataggcataa acggtgtccg
    16261 gcggcgggca ttcccccgat tttaccatcc cctgctctgc cagccattca cgcatgtaat
    16321 ggcccatata cacttcgagt acgccacctt tggtggtgag ttgcccaccg gggacgtccc
    16381 attcgggcca tttattcggc gtcgactgct ccagcacact gccattgttc gccagcggcg
    16441 cacgtaagtt atggcggctc atcatgagca cttgctgtag ctgatagcct tccggtacgg
    16501 tttgtgcctg agcgtttgaa gcgagtaaaa ctacccctgc cacagctgcg gcgattagcg
    16561 ttttgttcat tcctggcacc tcttttgtta tcaatcacta aagagtgtga cagaaatatg
    16621 acttttttcc gggaacaggt tcgcaaaatg gcttgttgaa cggctgtttt tatattcatc
    16681 tttaaaaaca ggcaaggttt gacgcaaact atgcctgatt agcccctgcc tcgcgtacgc
    16741 cgctgatgat tgcacctgtg gctaaacaga atatcggcgg cgttaccaat acagaggcaa
    16801 tatttaccaa taaaaaagtg tgagactaat aacaatggtt tttagcatat gcctctgtca
    16861 ttaaattgca taacgagtta aaaaaccaat ttgcgcattc ctttccttat tgtttaactc
    16921 gtaagcctga gcaaaaagtt caggagggag gtaaacgtca taatgattgc cagtgtttat
    16981 taaataacca tactcaagat cttcatctgt tgaaaaggat tcattcccta aggagattga
    17041 tgccacatta ctattaacaa taaataatga gcaaatgcta tctgaaagag cagtgaggaa
    17101 atttttcacc tcat
//
LOCUS       DBFIFS010000085        17021 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.085, whole genome shotgun sequence.
ACCESSION   DBFIFS010000085 DBFIFS010000000
VERSION     DBFIFS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.085"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            100..1239
                     /gene="arnB"
                     /locus_tag="N7A07_002507"
     CDS             100..1239
                     /gene="arnB"
                     /locus_tag="N7A07_002507"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU2477148.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1243..2211
                     /gene="arnC"
                     /locus_tag="N7A07_002508"
     CDS             1243..2211
                     /gene="arnC"
                     /locus_tag="N7A07_002508"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU2477149.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2211..4193
                     /gene="arnA"
                     /locus_tag="N7A07_002509"
     CDS             2211..4193
                     /gene="arnA"
                     /locus_tag="N7A07_002509"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU2477150.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4190..5080
                     /gene="arnD"
                     /locus_tag="N7A07_002510"
     CDS             4190..5080
                     /gene="arnD"
                     /locus_tag="N7A07_002510"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU2477151.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5080..6732
                     /gene="arnT"
                     /locus_tag="N7A07_002511"
     CDS             5080..6732
                     /gene="arnT"
                     /locus_tag="N7A07_002511"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU2477152.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6729..7064
                     /gene="arnE"
                     /locus_tag="N7A07_002512"
     CDS             6729..7064
                     /gene="arnE"
                     /locus_tag="N7A07_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU2477153.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7064..7450
                     /gene="arnF"
                     /locus_tag="N7A07_002513"
     CDS             7064..7450
                     /gene="arnF"
                     /locus_tag="N7A07_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU2477154.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7444..7710)
                     /gene="pmrD"
                     /locus_tag="N7A07_002514"
     CDS             complement(7444..7710)
                     /gene="pmrD"
                     /locus_tag="N7A07_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU2477155.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7820..9175)
                     /gene="menE"
                     /locus_tag="N7A07_002515"
     CDS             complement(7820..9175)
                     /gene="menE"
                     /locus_tag="N7A07_002515"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU2477156.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9172..10134)
                     /gene="menC"
                     /locus_tag="N7A07_002516"
     CDS             complement(9172..10134)
                     /gene="menC"
                     /locus_tag="N7A07_002516"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU2477157.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10134..10991)
                     /gene="menB"
                     /locus_tag="N7A07_002517"
     CDS             complement(10134..10991)
                     /gene="menB"
                     /locus_tag="N7A07_002517"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU2477158.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11006..11764)
                     /gene="menH"
                     /locus_tag="N7A07_002518"
     CDS             complement(11006..11764)
                     /gene="menH"
                     /locus_tag="N7A07_002518"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU2477159.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11761..13431)
                     /gene="menD"
                     /locus_tag="N7A07_002519"
     CDS             complement(11761..13431)
                     /gene="menD"
                     /locus_tag="N7A07_002519"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU2477160.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13520..14815)
                     /gene="menF"
                     /locus_tag="N7A07_002520"
     CDS             complement(13520..14815)
                     /gene="menF"
                     /locus_tag="N7A07_002520"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU2477161.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14894..15199)
                     /gene="elaB"
                     /locus_tag="N7A07_002521"
     CDS             complement(14894..15199)
                     /gene="elaB"
                     /locus_tag="N7A07_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU2477162.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15254..15715)
                     /gene="elaA"
                     /locus_tag="N7A07_002522"
     CDS             complement(15254..15715)
                     /gene="elaA"
                     /locus_tag="N7A07_002522"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2477163.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15780..16697
                     /gene="rbn"
                     /locus_tag="N7A07_002523"
     CDS             15780..16697
                     /gene="rbn"
                     /locus_tag="N7A07_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU2477164.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16885..>17021
                     /locus_tag="N7A07_002524"
     CDS             16885..>17021
                     /locus_tag="N7A07_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU2477165.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     E"
BASE COUNT         3966 a         4612 c         4340 g         4103 t
ORIGIN      
        1 tattctatcc ttaaaatttc gctctaaatg gcaaaatata cacaacactc tttatagcaa
       61 atataagtgg acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt
      121 tcgagaccag caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt
      181 tggatcacaa ccggtccgaa gaatcacgcg cttgaacaag ccttttgcca gttgacggga
      241 aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg
      301 ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc
      361 aatatgattt ccttgcaggg tgcaacgccg gtaatggtgg atgtcgatcg cgatacgctg
      421 atggtcacgc ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg
      481 gtgcattatg ccggtgcgcc agcagatatt aacgccattc gcgccattgg cgaacgttac
      541 ggcatcgcag ttatcgaaga tgctgcccat gccgtaggta cgtattacaa agggcgacat
      601 attggcgcaa aaggtaccgc tattttttca tttcatgcca ttaaaaatat tacctgtgcg
      661 gaaggaggcc tgattgttac tgataatgaa aatattgccc gccagctacg gatgctgaaa
      721 tttcacggcc tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct
      781 gaagtcttaa cgccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg
      841 acacagttag ccaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat
      901 cagcaagcac tggcagctct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt
      961 cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat
     1021 gcgttgatgg aagcgttaaa agaaagaggt attggtaccg ggttacattt ccgcgccgct
     1081 cacacacaaa aatattatcg cgagcgtttt ccctcgctgt cgttaccgaa taccgaatgg
     1141 aatagcgaac gcatctgttc tttgccgctg ttcccggata tgactaccgc cgatgccgac
     1201 cgtgtcatca cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct
     1261 gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta
     1321 atcaggcgca ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt
     1381 gatgacggca gtagcgataa ttccgcgcat atactggtcg aagcctcaca agcggagaac
     1441 agccatattg tatctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg
     1501 ggattcagtc acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg
     1561 ccagaagaaa tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact
     1621 gtacgccaga accgccagga cagctggttt cgtaaaacag cttcgaagat gattaaccgg
     1681 cttattcagc gcaccaccgg caaagcgatg ggtgactacg gttgtatgct gcgcgcctat
     1741 cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt
     1801 ctggcgaata tcttcgcccg ccgtgccatt gaaattccag tgcatcatgc cgagcgtgag
     1861 tatggtgaat ccaaatacag ctttatgcgc ctgattaatt tgatgtacga cctggtgacc
     1921 tgccttacca caacgccgct acgtatgctg agcctgctcg gcagcattat tgcgattgga
     1981 ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg
     2041 gcagaaggcg tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc
     2101 ggcatgggat tactcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc
     2161 cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg
     2221 tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg gctgccggtt
     2281 acgaaattag cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt
     2341 cggtggctcg tctggcggcg gaaagaggca ttccggttta tgcgccggat aacgttaatc
     2401 atccgctgtg ggtggaacgc attgcccaac tctcaccaga ggtgattttc tctttttatt
     2461 atcgccatct tatttgcgac gagattttgc agctcgctcc cgcaggtgca tttaatctgc
     2521 acggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg
     2581 gtgaaacaga aactggcgtt acattgcacc gaatggtaaa acgtgccgat gccggggcca
     2641 ttgtggcgca actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata
     2701 aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta
     2761 atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg
     2821 atgacagttt cctcgaatgg cataagccgg catccgtact gcacaacatg gtacgtgccg
     2881 ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt
     2941 cgtcgcgtgt tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc
     3001 cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt caccggacaa gcgggcgacg
     3061 gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa ggttcacgct
     3121 tgaatagcca gcctgcctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga
     3181 atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt
     3241 acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg cattttcact
     3301 ttgtcgaagg cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg
     3361 atgtcgtctt gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc
     3421 gcgtatttga actcgatttt gaagagaatc tgcgcattat ccgctactgc gtgaagtacc
     3481 gtaagcgaat catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact
     3541 tcgatgagga ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt
     3601 cggtatcaaa acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac
     3661 agttcaccct cttccgcccg tttaactgga tgggaccacg actggataac cttaatgcgg
     3721 cacgaatcgg cagctcccgc gcgattacgc aactcattct caatctggta gaaggttcac
     3781 cgattaagct gattgatggc ggaaaacaaa aacgctgctt tactgatatt cgcgatggta
     3841 tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca
     3901 acattggcaa tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga
     3961 gcttcgaaaa acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg
     4021 aaagtagcag ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc
     4081 gcaatgccca ccactgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg
     4141 aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt
     4201 aggcttacgc attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct
     4261 ggaaatcttg agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa
     4321 tatgggccgc catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc
     4381 aaacgcggca tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa
     4441 agagattggt catgccaatg ccgatatcat tcgtgaagcg gctaaatatc acgaagtcgg
     4501 cctgcacgcc tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac
     4561 aatgatcgac gatattgcac gcggccttcg cactctggaa gagattatcg gtcaaccggt
     4621 aacctgttct gccgctgcgg gctggcgtgc cgaccagcag gtgatcgaag caaaagaagc
     4681 gttccatttg cgctacaaca gcgattgtcg tggggccatg ccgttccgtc cactgctcga
     4741 atcaggaaac cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat
     4801 tggtcgggat gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga
     4861 taaaggcacg ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa
     4921 ttttgtggat ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga
     4981 actgttatca gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga
     5041 aggctggctg ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt
     5101 atcggcctct tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgcta
     5161 tggcaacccg atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac
     5221 tggattgtgc cccatctgtt agggctacgt tattttgaaa aacccattgc cggatactgg
     5281 attaacagca ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt
     5341 atctttgcga ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc
     5401 gataaacgtc tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc
     5461 atcggcactt atgccgtgct cgatccgttt attgccttct ggctggtggc gggaatgtgc
     5521 agcttctggc tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg
     5581 ctgggaatca cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg
     5641 gtattaagcg tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac
     5701 ggctggctgg cggtcatcag ttgcgtactg acggttctcc cctggggact gacgatagcg
     5761 cagcgggagc ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca
     5821 ctggatgatg cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt
     5881 agtctgccgt ggctgggatt actccccggt gcactataca caggctggaa aaaccgcaag
     5941 cattccgcaa ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtt
     6001 gctaagggta aattgcccac ctatattctt tcctgctttg cacctttggc aatgctgatg
     6061 gcgcattacg ctttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg
     6121 attaacatcg cttttggcgt cactggcatt attgccacat ttgtggtctc cccctgggga
     6181 ccaatgaaca cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg
     6241 attttttcgc tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg
     6301 tcttttgccg cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac
     6361 agagtcatgg aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag
     6421 ccaagccgct atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagtctg
     6481 caacgcgatg acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat
     6541 ccggatgcga aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat
     6601 cgtcaggagg ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt
     6661 ctcgccattc cgcccgcaga tgccatcgat cgtcaggagc gtctggtgct gattcagtat
     6721 cgtcccaaat gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt
     6781 tgtgtcagaa acaggcaacc tgctttgtgg cgataagcaa acggcgcaaa catatcgtgc
     6841 tgtggctggg actggcgctg gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct
     6901 tgcagaacgt accggtaggt attgcctacc cgatgttaag tctgaatttt gtctgggtga
     6961 cgctggctgc agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg
     7021 cgttcattat tggtggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatatgggg
     7081 attattcagc gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcgagtca
     7141 tctgccgccg atgacgcact tatgggattt tatcgcggcg ctgttagctt ttgggctgga
     7201 tgcccggata ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa
     7261 aacactacat aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct
     7321 ggtatggatt gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact
     7381 actgggagta gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca
     7441 acgttactga gttttccctg ccactttaca ctgccgttcc cactcatcgg ggctataaca
     7501 gcttgcagag aggactttca ccgtatgcag cttttcacga ttaatacaat aaagcgcatt
     7561 ctgcaaaggc gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat
     7621 catttttatc gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt
     7681 gcagcacgat tttttgacca gccattccat tgtattatcc tgtttgctaa gagttttcat
     7741 atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg cagaactgag
     7801 aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg
     7861 aaattttaat accgccgttt ttcagctccg gcggcaaagt cagccaacgt accggttgct
     7921 gaaaacgggc cagcttatct ttcacccatt cactaagatc aacgctctcg tggtcatact
     7981 ccatcaccgc caccggtcga tgaccaaact ccttgtcggc aacggggacg ataaacacct
     8041 gcaaaaccgc aggatgtgca gcaattacgc gctcgacttc ttccgtctga ataccctctc
     8101 cgccactgaa gaatagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct
     8161 caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt
     8221 tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt
     8281 tcacttcccg acccggcagc ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac
     8341 acaccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc
     8401 gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc gctttcaggg
     8461 aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag
     8521 tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag
     8581 cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca
     8641 accagtcatc gtgatcgcca aacggaatca gcgataacac cccttcggca ctggcaagat
     8701 gggcctgata agtatggaca gcggctttcg gcaacccggt agaacccgag gtcaacgtca
     8761 ttgagcacag acgcgtcggc tgccacgtag cgacatgtgc gccttcaacc cgctgaatgt
     8821 gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg
     8881 tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca
     8941 cccgcgcccc gcattgcagt aacgccagcc aagcgagcag cgtttgcggc gtattccacg
     9001 cccgcaacat cacgccgctg tcctcaacca caccctgcac cgcaaatccg gaggctaatt
     9061 catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga
     9121 tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa
     9181 ccgctccagt gcatcaactt ccacgacagg cagcgtgcta cccggccagc gacgtacctg
     9241 ctgcgcctgc attagatcca gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc
     9301 agcaatccgc gccagttgcg ttaagcctaa gctcgattca atggaagaac tgatcactgc
     9361 cgtcagcccc agcgcgtgcg ccgcctgtac ctgctcgcga actttctcca gactgcccgt
     9421 gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg
     9481 ctcgcgcaag ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga
     9541 atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat aatccgggtt
     9601 aacgtatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg
     9661 caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc
     9721 ttcgtacaat cccactttga ccttcgccac tttctcgcct ggcatatctg caagtttgag
     9781 gatcagatcg tccggatcgc cattacacag tggtgccgta cggtaattgg ccgcctgcgg
     9841 gagcgtatct gccagctccg ccaatgcaca acttacgcca aaggcaacgg aaggcatctg
     9901 cggtaattcg caatcgcctg ccagccagtt atttacccag gcaagcagca cactttgcgc
     9961 ctcttcccag gtttcctgac tgaagcccgg cagtggggag atctcccccc acccttcgcg
    10021 ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag
    10081 aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga
    10141 ttccgtttga atttgctgaa gtcaggctga cgtttctggt tgaaggcgtt gcgaccttcc
    10201 tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagacct
    10261 gcctgaccgt cacagtcggc gttcagtgca gctttcaggc agcgcagcgc catcggactg
    10321 ttttgcagca tttcacggca ccagcgaacg gtttcttttt ccagatccgc cagcggtacc
    10381 acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac
    10441 cagatttcac gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg
    10501 ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgccgcgata
    10561 gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggaatagcc agccaccatc
    10621 gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga
    10681 tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg
    10741 gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgacgtt atcgtcataa
    10801 cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc
    10861 acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg
    10921 tcctcaaagc cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca
    10981 ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta
    11041 cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg
    11101 cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa
    11161 acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg
    11221 cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcacc accagctccc
    11281 ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg
    11341 cggttaatgg ttcggaacga aaacgctgcg cccattggcg atcggaacgc tgacgttccg
    11401 cgcgttgttc agcattttgc agccccggat gaccgccttc gacaacaacc ccacaaagcc
    11461 ccgccagccc ctggcaagcg gccatcatcg ccacccgtcc gccaagcgag taccccacca
    11521 gccagaagct aaggatgttg taactaacca gggttttacg cagtaagtcg gtgacatcat
    11581 caaatccatc gacgctaatc gccgccgaac caccgtgacc tgggagatca acatacaacc
    11641 gtgagtagtc ggcaaacgcc tcgccgactt cctgccattc gaggcaatcg ccggaaaaac
    11701 cgtgcaaaaa caccagccag ggtaaacctg gtttgccgtg ttttgcctgc gcgtgcagga
    11761 tcataaatgg cttacctgcg ccagcagctg ctggagcgtt tgcgcgccgt cggtgtcgtt
    11821 aaccaccatt tcaattaccg tggtggtcgg agtacgccag gcgtcggcaa gtaccgtttc
    11881 aagttcctgc cagttttgcg gacgatgata tttcagctca aacatcgcgg cagcgtgctc
    11941 aaaatggacg ttttgcggca tcagatagaa acgctcgcgc tcgcttttcg gcgttggcag
    12001 cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcggaaac
    12061 ctggcgcaat aacgccagcg cattcaggtc gtaaagtgcg gagagatcgc ccacaatcgc
    12121 cagcgtcggt ttgccgcttg cccgctgaac gccggcggca gtcgagagca gcccgtcgat
    12181 accgctggcc ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc
    12241 aatcagacgt accaccaggc tgttaccgac aaacaattgc ccctgttcag gcagatagtc
    12301 gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatcg cgacaggcaa taaccgcctg
    12361 cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg
    12421 atgcagttcc agccagtcgg caatattggc aattaagcgg cgtccacgat ggtgtgccgg
    12481 atcaagccgc ccttcgatgt catccacaat ccagtactct tcgggttcac agcttgcctg
    12541 ccattgcagg agccgtttgc ccgtcaggct gcttcccagt tgcaccacaa tttgcgcctg
    12601 ttgcagttcg ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg
    12661 tccggtttgc gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagggc
    12721 aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgccg
    12781 ccagaagaac cagtcgcgct gtttttcgct ctcaagacga ggcgcttcac gcagccacgg
    12841 cttatcatcc tgccaccagt cacccagacg ctgttgccag ctaagcccag tgtcgtccat
    12901 ctcgccatac agcggttcag caaacgggca gttgatatgg accccaccag catgaagcgt
    12961 accgagagca tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg
    13021 cggcagtgaa atactgtgcg tggggtgaga ggcgaacatt cccggctgac gaatcgcctg
    13081 attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaacagga tcagtttttc
    13141 tccggttaac ccagcttcaa tcagtgccgg atagagattt gctaccgccg tgccggaggt
    13201 cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccag
    13261 tccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt
    13321 taacggtgta gaacgcgagc caggggcgat gcagatgtgt cttacgccgt gacgcgttaa
    13381 tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc
    13441 cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg
    13501 gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt
    13561 tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc gcgccagcat
    13621 atagccgcac gacattgccg ctgatttttg ctgagcgcag ggaaacgcag aattcgcttt
    13681 gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgat
    13741 gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca
    13801 actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa
    13861 gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat
    13921 cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc tggtttttat
    13981 catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta
    14041 ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg
    14101 gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattga
    14161 gattcagtcg acgactggca gccatcatcg ccgccgcatt gaccggactt gcgaaatgca
    14221 ggtcagttgc ccgagcaagc accactttgt cgagctcacc ttcggcgatg gttttcgttg
    14281 ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtagtgg
    14341 ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tctcttgcct
    14401 taatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac
    14461 cgccacagcg tcgccattcc aggcgtggta aaagtaaatt gccctgcgac gggtcaaacg
    14521 cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg
    14581 cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat
    14641 tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat
    14701 caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa
    14761 tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga
    14821 cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg
    14881 gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagccca acggccgcac
    14941 ccacaccaat tccctgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct
    15001 gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca
    15061 gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg
    15121 agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat
    15181 caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga
    15241 caagggttat tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat
    15301 cttcttcata gatctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat
    15361 gcgcctgcgc cccgaggtag acaggcttat caggccagtg atgcgtgcaa gtgtcgaggg
    15421 ttttactcat cagttgctgc cccacttttt cgccacgcaa cgcttcactg acaatcaccc
    15481 gaccaataac gaccggctca agatcatcat cacttttcag aatcctcgca tatgccacca
    15541 gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca tcaccatcga
    15601 tatcctgata agggcaattt tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg
    15661 cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc
    15721 tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt gatgaactca
    15781 tggaattaat ttttttaggt acttcggccg gtgtgccaac ccgcacgcgc aatgtcacgg
    15841 caatattgct aaacctgcaa catccgaccc agagcggact ttggttgttt gactgcggtg
    15901 aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt
    15961 ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt
    16021 ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc cgtgaatttg
    16081 tggagaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa
    16141 ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt gaccgcttat ccaatggaac
    16201 acccactgga atgctatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg
    16261 cccaggcatt aaaagctgct ggcgtaccgc ctagcccact gtttcaggaa ttaaaagcgg
    16321 gcaaaacaat catgctggaa gatggaaggc agattaacgg cgcagattac ttagctgctc
    16381 cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcacttg
    16441 acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag
    16501 ccaaagccaa tagtcgcggt catagctcta cacgccaggc tgcgacactg gcccgtgagg
    16561 ctggagtcgg taagctaatc attacccacg tcagctcgcg ctatgatgac aaaggttgtc
    16621 agcacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactagcg aatgatttca
    16681 ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc
    16741 gcaacccgcg ctacatatat ccactcacac gggcatattg agcgattttt gatatttatt
    16801 catctcctta cgcactatat tcagagcaat aagttgcctc tttttatctt cattcagcac
    16861 aacctttgca actaaaagga gcatatgatg gttacagttg tcagcaatta ttgtcaatta
    16921 tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga ggaattactg
    16981 cagtctttaa aaaaaacagc gttatccgga gatgaagaaa g
//
LOCUS       DBFIFS010000086        16904 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.086, whole genome shotgun sequence.
ACCESSION   DBFIFS010000086 DBFIFS010000000
VERSION     DBFIFS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.086"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..253
                     /locus_tag="N7A07_002525"
     CDS             <1..253
                     /locus_tag="N7A07_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2477166.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="N7A07_002526"
     CDS             265..843
                     /locus_tag="N7A07_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2477167.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="N7A07_002527"
     CDS             840..1235
                     /locus_tag="N7A07_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2477168.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="N7A07_002528"
     CDS             1243..1983
                     /locus_tag="N7A07_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2477169.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="N7A07_002529"
     CDS             1999..2421
                     /locus_tag="N7A07_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2477170.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="N7A07_002530"
     CDS             2403..2837
                     /locus_tag="N7A07_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2477171.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="N7A07_002531"
     CDS             2830..5379
                     /locus_tag="N7A07_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2477172.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="N7A07_002532"
     CDS             5376..5705
                     /locus_tag="N7A07_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2477173.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..6403
                     /locus_tag="N7A07_002533"
     CDS             5705..6403
                     /locus_tag="N7A07_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2477174.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            6409..7152
                     /locus_tag="N7A07_002534"
     CDS             6409..7152
                     /locus_tag="N7A07_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2477175.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            7149..7721
                     /locus_tag="N7A07_002535"
     CDS             7149..7721
                     /locus_tag="N7A07_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2477176.1"
                     /translation="MKTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7782..11180
                     /locus_tag="N7A07_002536"
     CDS             7782..11180
                     /locus_tag="N7A07_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU2477177.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            11247..11846
                     /locus_tag="N7A07_002537"
     CDS             11247..11846
                     /locus_tag="N7A07_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2477178.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11911..14826
                     /locus_tag="N7A07_002538"
     CDS             11911..14826
                     /locus_tag="N7A07_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2477179.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            14826..15407
                     /locus_tag="N7A07_002539"
     CDS             14826..15407
                     /locus_tag="N7A07_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2477180.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(15527..16417)
                     /locus_tag="N7A07_002540"
     CDS             complement(15527..16417)
                     /locus_tag="N7A07_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGU2477181.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(16436..>16904)
                     /locus_tag="N7A07_002541"
     CDS             complement(16436..>16904)
                     /locus_tag="N7A07_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2477182.1"
                     /translation="AHAMEKQAEKMLESMASRIENYPELRSRIEQHISETKNQLSQLE
                     SILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYVFEQFEIACYTSLL
                     AAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSEIDGVEAKK"
BASE COUNT         4072 a         4179 c         5073 g         3580 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag agtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcttg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt
     6001 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     6061 caacggaaac agtgacgccg atccggagca ggaggtgatc agccgctggc gcattgagca
     6121 gtgcagcgaa ctgagcgcgg tgagtgcctc ttttgtactg tccacgccga cggaaacgga
     6181 cggcgctgtt tttccgggac gtatcatgct ggccaacacc tgcacctgga cctatcgcgg
     6241 cgatgagtgc ggttatcacg gtccggcggt cgcggatgaa tatgaccagc caacgtccga
     6301 tatcacgaag gataaatgca gcaaatgcct gagcggttgt aagttccgca ataacgtcgg
     6361 caactttggc ggcttccttt ccattaacaa actttcgcag taaatcccat gacacagaca
     6421 gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg cggcttcgtg
     6481 gtaagcacgc cggaggggga aagatatttc ccctgcgtga atatctccgg tgagccggag
     6541 gcgtatttcc gtatgtcgcc ggaagactgg ctgcaggcag aaatgcaggg tgagattgtg
     6601 gcgctggtcc acagccaccc cggtggtctg ccctggctga gtgaggccga ccggcggctg
     6661 caggtgcaga gtgatttgcc gtggtggctg gtctgccggg ggacgattca taagttccgc
     6721 tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg ttacacgctg
     6781 ttccgggatg cttatcatct ggcggggatt gagatgccgg attttcatcg cgaggatgac
     6841 tggtggcgtc acggtcagaa tctctatctg gataatctgg aggccacagg gctgtatcag
     6901 gtgccgttgt catcagcaca accgggcgat gtgctgctgt gctgttttgg ttcatcggtg
     6961 ccgaatcatg ccgccattta ctgtggtgat ggcgagctgc tgcaccatat tcctgaacaa
     7021 ctgagcaaac gagagaggta taccgacaaa tggcagcgac gcacacactc cctctggcgt
     7081 caccgggcat ggcgcgcatc tgcctttacg gggatttgca acgatttggc cgccgcatcg
     7141 accttcgtgt gaaaacgggg gctgaagcca tccgcgcact ggccacacag ctcccggtgt
     7201 ttcgtcagaa gctgagcgac ggctggtatc aggtacggat taccgggcgg gacgtcagca
     7261 cgtccgggtt aacggcgcag ttacatgaga ctctgcctga tggcgctgtg attcatattg
     7321 ttcccagagt cgccggggcc aagtcaggtg gcgtattcca gattgtcctg ggggctgccg
     7381 ccattgccgg atcattcttt accgccggag ccacccttgc agcatggggg gcagccattg
     7441 gggccggtgg tatgaccggc atcctgtttt ctctcggtgc cagtatggtg ctcggtggtg
     7501 tggcgcagat gctggcaccg aaagccagaa ctccccgtac acagacaacg gataacggca
     7561 aacagaacac ctatttctcc tcactggata acatggttgc ccagggcaat gttctgccgg
     7621 ttctgtacgg tgaaatgcgc gtggggtcac gcgtggtttc tcaagagatc agcacggcag
     7681 acgaagggga cggtggtcag gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga
     7741 aaccgcctcc gggcggtttt atcgtttatg gagcatgacg aatgggtaaa ggcagcagta
     7801 aggggcatac cccgcgcgaa gcgaaggaca acctgaagtc cacgcagctg ctgagtgtga
     7861 tcgatgccat cagcgaaggg ccggttgaag gtccggtgga tggattaaaa agcgtgctgc
     7921 tgaacagtac gccggtgctg gacagtgagg ggaataccaa tatatccggc gtcacggtgg
     7981 tgttccgggc cggtgagcag gagcagacac cgccggaggg atttgaatcc tccggctccg
     8041 agacggtgct cggtacagaa gtgaaatatg acacgccgat cacccggacc atcacgtcgg
     8101 caaacattga ccgtctgcgt tttactttcg gcgtgcaggc actggtggaa accacctcaa
     8161 agggggacag gaatccatcg gaagtccgcc tgctggttca gatacaacgt aacggtggct
     8221 gggtgacgga aaaagacatc accattaagg gtaaaaccac ttcacagtat ctggcctcgg
     8281 tggtggtgga taacctgccg ccgcgcccgt ttaatatccg gatgcgcagg atgacgccgg
     8341 acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcatacacc gaaattatcg
     8401 atgtgaaaca gtgctacccg aacacggcac tggtcggcgt gcaggtggac tcggagcagt
     8461 tcggcagcca gaaggtgagc cgtaattatc atctgcgcgg gcgtattctg caggtgccgt
     8521 cgaattataa cccgcagacg cggcaataca gcggtatctg ggacggaacg tttaaaccgg
     8581 catacagcaa caacatggcc tggtgtctgt gggatatgct gacccatccg cgctacggca
     8641 tggggaaacg tcttggtgcg gcggatgtgg ataaatgggc gctgtatgtc atcggccagt
     8701 actgcgacca gtcagtgccg gacggctttg gcggcacgga gccgcgcatc acctgtaatg
     8761 cctacctgac cacacagcgt aaggcgtggg atgttctcag cgatttctgc tcggcgatgc
     8821 gctgtatgcc ggtatggaac gggcagacgc tgacgttcgt gcaggaccga ccgtcggata
     8881 aggtgtggac ctataaccgc agtaatgtgg tgatgccgga tgatggcgcg tcgttccgct
     8941 acagcttcag cgccctcaag gaccgccata atgccgttga ggtgaactgg attgacccgg
     9001 ataacggctg ggagacggcg acagagcttg tggaggacac gcaggccatt ctccgttacg
     9061 gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag ccgggggcag gcacaccgcg
     9121 ccgggctgtg gctgattaaa acggagctgc tggagacgca gaccgtggat ttcagcgtgg
     9181 gcgcagaagg gcttcgccat gtaccgggcg atgtcattga aatctgtgat gatgactatg
     9241 cggggatcag catcggtggg cgtgtgctgg cggtgaacag ccagacccgg acgctgacgc
     9301 tcgaccgtga aatcacgctg ccatcctccg gtaccacgct gataagcctg gttgacggaa
     9361 gtggcaatcc ggtcagcgtg gaggtccagt ccgtcaccga cggcgtgaag gtaaaagtga
     9421 gccgtgttcc tgacggcgtt gccggataca gcgtatgggg gctgaagttg ccgacgttgc
     9481 gccagcgcct gttccgctgc gtgagtatcc gtgagaacga cgacggcacg tatgccatca
     9541 ccgccgtgca gcatgtaccc gaaaaagaag ccatcgtgga taacggggcg cactttgacg
     9601 gcgacctgag cggcacggtg aatggcgtca cgccgcccgc ggtgcagcac ctgactgccg
     9661 aagtcaccgc agacagcggg gaatatcagg tgctggcgcg ctgggacacg ccgaaggtgg
     9721 tgaagggggt gagcttcctg cttcgcctga ccgtggcagc ggacgatggc agtgagcggc
     9781 tggtcagtac ggccaggacg acggaaacca cataccgctt cacgcaactg gcgctgggga
     9841 actacaggct gactgtccgg gcggtaaatg cgtggggaca gcagggcgat ccggcatcgg
     9901 tatcgttccg gattgccgca ccggcagcgc cgtctcggat tgagctgaca ccaggctatt
     9961 ttcagataac cgccacgccg catcttgcgg tttatgatcc gacggtacag tttgagttct
    10021 ggttctcgga aacgcggatt gcggatatca ggcaggttga aaccagcgcg cgttatcttg
    10081 gtacggcgct gtactggata gccgccagta tcaatatcaa accgggccat gattattact
    10141 tttatatccg cagtgtgaac accgttggca aatcggcatt cgtggaggcc gtcggtcggg
    10201 cgagcgatga tgcggaaggt tatctggatt ttttcaaagg caagataacc gaatctcatc
    10261 ttggtaaaga gctactggaa aaagttgacc tgacggagga taacgccagc agactggatg
    10321 agttttcgaa agagtggaag gatgctaacg ataagtggaa tgccatgtgg ggcgtcaaaa
    10381 ttgagcagac caaagacggc aaacattatg tcgcgggtat tggcctcagc atggaggaca
    10441 cggaggaagg caagctgagc cagtttctgg ttgccgccaa tcgtatcgcg tttattgacc
    10501 cggcaaacgg gaatgaaacg ccgatgtttg tggcgcaggg caaccagata ttcatgaacg
    10561 acgtgttcct gaagcgcctg acggccccca ccattaccag cggtggaaat ccaccggcat
    10621 tttccctgac accggacgga aagctgactg ctaaaaatgc ggatatcagt ggcagtgtga
    10681 atgcgaactc cgggacgctc aacaacgtca cgattaatga gaactgtcag attaagggga
    10741 aactgtcagc caatcagatt gaaggcgata ttgtcaaaac ggtcagcaag tctttccccc
    10801 gcacgagcac ttatgccagt ggcaccatca cggtaagaat cagtgatgat cagaaatttg
    10861 accggcaggt catgataccg ccagtgttat tccgcggtgg taagcatgag aatttcaaca
    10921 gtaataacca acagtcatac tggtattcaa cctgccggtt aagagtgacc cgcaatggcc
    10981 aggagatttt taatcagtcc acgacggatg ctcagggcgt attttcctca gttatagata
    11041 tgcctgccgg acaggggacg ctgacactga cattcaccgt atcttcatca ggagcgaata
    11101 actggacacc aacaaccagt atcagcgatc tgctggttgt ggtgatgaaa aaatccacag
    11161 caggtatcag tatcagctga attttataac ccagaacggg cgtcagaaat gacgcctttt
    11221 ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgccgccatt ttgtccgcag
    11281 ccatttgtct ggccgtatcc ggtgcgcctg catgggcatc tgaacatcag tccacgctga
    11341 gcgcggggta tcttcatgcc cggaccaacg ttcccggcag tgatgatctg aacgggatta
    11401 acgtgaaata ccgttatgag tttacggata cgctggggct ggtgacgtca ttcagctatg
    11461 caggagacaa gaatcgccag ctgacccgtt acagcgatac ccgctggcat gaagattccg
    11521 tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat gaatggttca
    11581 gcgcgtatgc gatggcgggt atggcttaca gccgtgtttc gactttttcc ggggattacc
    11641 ttcgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccggaagt gatgacggtc
    11701 gccacagcaa cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg
    11761 tggccattga tattgcttat gaaggttctg gcagtggtga ctggcgcact gacgggttca
    11821 tcgtgggtgt tggttataaa ttctgattag ccaggtaaca cagtgttatg acagcccgcc
    11881 ggttcaggcg ggcttttttg tggggtgaat atggcagtaa agatttcagg tgtactgaaa
    11941 gacggcacag gaaaaccggt acagaactgc acaatccagc tgaaagcaaa acgtaacagt
    12001 accacggtgg tggtgaacac gctggcctca gaaaatccgg atgaagccgg gcgttacagc
    12061 atggacgttg agtacggtca gtacagcgtt attctgttgg tggaagggtt cccgccgtca
    12121 catgccggga ccatcaccgt gtatgaagat tctcaacccg gtacgctgaa tgattttctt
    12181 ggtgccatga ctgaggatga tgtccgtccg gaggcactgc gccgttttga gctgatggtg
    12241 gaagaggtgg cgcgtaacgc gtccgcggtg gcacagaaca cggcagccgc gaagaagtca
    12301 gccagcgatg ccagcacatc agcccgtgag gcggcaaccc atgcgactga tgctgcggac
    12361 tcagcacgcg cagccagcac gtcagccgga caggccgcgt cgtcggctca gtcagcgtct
    12421 tccagcgcag gaacggcatc aacaaaggct actgaagcat caaaaagtgc tgccgctgca
    12481 gagtcctcaa aaagcgcggc ggctaccagt gccggtgcgg cgaaaacgtc agaaacgaat
    12541 gcggcagtgt cacaacaatc agccgccact tctgcatcca ccgcgaccac gaaagcgtca
    12601 gaagctgcct cctcagccag ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa
    12661 acgagcgcag cctcgagcgc cagtagtgca gcctcctcgg caacggcggc aggcaattcc
    12721 gcgaaggcgg ccaaaacgtc tgagacaaac gctaagtcct ctgaaacggc agcagaacag
    12781 agtgcctccg cagcagcagg ctcaaaaaca gcggctgcat tatctgccag tgccgcgtca
    12841 acaagtgccg ggcaggcctc agccagtgcc accgccgccg gaaaatcggc agaaagtgcc
    12901 gcatcgtctg cttcaacagc cacaacgaag gctggcgaag ccactgaaca ggccagcgca
    12961 gcagcgagtt ctgcttccgc agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc
    13021 agcgcagaat cctcaaaaac ggctgccgca tcgtcagcca gttcggcggc gtcatcggca
    13081 tcatctgcgt ctgcttcaaa agatgaggcg accagacaag cgtcagcagc gaagagcagc
    13141 gccacgacgg catccacgaa ggcgacagag gcagctggta gtgcgacggc agcagctcag
    13201 agcaaaagta cggcggaatc tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag
    13261 gatattgcat ccgccgtggc gcttgaggat gcgagcacga cgaaaaaggg gatagtacag
    13321 ctcagcagtg cgactaacag cacttccgag tcactggcgg caacgccaaa agccgttaag
    13381 gccgcgtatg agctggctaa cgggaaatac accgcacagg atgcaacgac agcacagaaa
    13441 gggatagttc agcttagcaa cgcgaccaac agcacatctg aaatgctggc ggcaacgcca
    13501 aagtcggtaa aggcagccta tgaccttgct aacgggaaat atactgctca ggacgctacg
    13561 acagcacaaa aaggaattgt ccagctcagt agtgcaacca acagcgcatc tgaaacgctt
    13621 gccgcgacac cgaaagcagt gaaagcagct aatgataatg cgaatggtcg ggtaccttct
    13681 gcccgtaagg tgaatggtaa ggcgctttca tcggatataa cactgacgcc gaaagatatt
    13741 ggtacgctta actcaacaac aatgtcattc agcggtggtg ctggttggtt caaattagca
    13801 acggtaacca tgccacaggc gagttctgtt gtttcaatta cgttgattgg tggcgcggga
    13861 tttaacgtgg ggtcacctca acaggcaggt atatctgaac ttgttttgcg tgcaggtaat
    13921 ggtaatccga aggggattac tggtgcttta tggcagcgca catcgacagg gtttacaaat
    13981 tttgcctggg tcaatacatc tggtgatact tacgatattt acgttgcaat cggaaattat
    14041 gcgactggtg taaatattca atgggattat accagtaatg ccagcgtgac gattcatacg
    14101 tcaccagcat attctgctaa taagccggaa gggttaacgg acggtacagt ttattcactc
    14161 tatacgccat cagagcagtt ttatccgcct ggcgcaccaa tcccgtggcc atcagatacc
    14221 gttccgtctg gctatgccct gatgcagggg cagacttttg acaaatctgc atacccgaaa
    14281 cttgcagccg cttatccgtc aggcgtgatc cctgatatgc gtggctggac gattaagggc
    14341 aaacctgcca gtggtcgggc cgtattgtct caggaacagg acggcattaa atcgcacacc
    14401 cacagcgcca gcgcatccag tacggatttg gggacgaaaa ccacatcgtc gtttgattac
    14461 ggcactaaat ccacgaataa caccggggcg cacacgcaca gtgtgagcgg tacagccgca
    14521 agtgccggaa accatactca tagtgtcaca ggcgcatcag cagtcagcca gtggtcacaa
    14581 aatgggtcag tacataaggt agtgtctgcg gccagtgtga atacaagtgc tgcaggagcg
    14641 cacactcata gtgtcagcgg cacagctgca tctgcaggtg ctcacgcaca tactgtcggt
    14701 attggtgctc atacgcactc tgttgcgatt ggctcacatg gacacaccat caccgttaac
    14761 gctgcgggta acgcggaaaa cactgtcaaa aacatcgcat ttaactacat tgtgaggctt
    14821 gcataatggc attcagaatg agtgaacaac cacggaccat aaaaatttat aatctgctga
    14881 ccggaactaa tgaatttatt ggtgaaggtg acgcatatat tccgcctcat acaggtctgc
    14941 cagcaaacag tacctatatt gcaccgccag atattccggc tggcttcgtg gctgttttca
    15001 acagtgatga gtcatcgtgg catctcgttg aagatcatcg gggtaaaacg gtttatgacg
    15061 tggcttccgg cgacgcgtta tttatttctg aactcggctc attaccggaa aatgttacct
    15121 ggttatcgcc ggaaggggag tttcagaagt ggaacggcac agcctgggtg aaagatgcag
    15181 aagcagaaaa actgttccgg atccgggagg cggaagaaac aaaaaacagc ctgatgcagg
    15241 tagccagtga gcatattgcg ccacttcagg atgctgtaga tctggaaatc gcaacggagg
    15301 aagaaacctc attgctggaa gcctggaaaa agtatcgggt gttgctgaac cgtgttgata
    15361 catcaactgc acctgatatt gagtggcctg taatacctga agtttaaaat aaaagcccgc
    15421 ttaaaatatc gcgggcttcg aaatataaat gtattttcag aggctagagc ttaggatatc
    15481 tcagcggcaa ttaacttatg agggatagct aaataccaga agataattag tttttactat
    15541 ttatctttgt ttgtggttct ccttcagcaa gctcagcgcc agtgacagga ttgatgtctt
    15601 catgggattt caaccttgtt gccatagctt ttatcatagc ttcttgcttg gtcgaaagat
    15661 ttatcgaggc cttcccatcc ccaccgtcaa ccgctgaaac tggatcggaa acatagtcaa
    15721 agttttcatc actgttccaa ctacctctga cgtcttcacc ttctgacatg ttgtaatata
    15781 cgtttttgta tttctcaagt ggtggtaatt ttcctggtgg aaaggtgtta cgaattgaat
    15841 gtaatgcttt ttcaaatgaa agcatgtgtg ctgcttcccg ggtcattaaa aatgccagag
    15901 tgtcttttac tccaggatca tcagtaacat taatgagacg ttcgtaaatg atctttgccc
    15961 gagcttcagc tgcaatattt gaacgaagat cggccgtgac ttcgccaata gtatcaacat
    16021 aagctgcagt ccagggtact ccagctgaat tagttaatgc tgggcctcct ccgtagagga
    16081 gagaagttat atggctatca ttgccatttt gagttaagga acggtaaagc tcagcttcat
    16141 tctcagttcc ttcagcaagt gctccttttg cacctttgtt gagcatacca acaagagaac
    16201 caataatttc gagatgactt agttcttctg ttgctatgtc catcagcata tcccttcggc
    16261 ctgcatcttc atcacttaag ccttgagtga agtatcggca tgctgctgca agctcaccct
    16321 gtggcccacc gaattgttct aagagtagat tagccaagcc tgggtttggc tcacttacac
    16381 gtactgtata ttgaagtttt ttcacgtgtc taaacataat gcctcctcaa aagaattatt
    16441 ttttggcttc aactccatca atttcagatc tgaccatgaa ttgctcagtg gtgtcaggaa
    16501 tatggttaag aagccattca gccatttgtt tttcttcatt aagaattgct tcaataatgg
    16561 gtactgaagc tgtatcaccc gcatttttgg ctgctgcgag aagagatgtg taacacgcaa
    16621 tttcaaattg ttcgaacaca tagccactga tcgaaccttt gacaatttca tcagatggaa
    16681 aaattccacc aatggattgt ccaaacgcgg ccattttact catagagtct ttaatgacag
    16741 agcgcgaaat attattacga tcaagtatgc tttcaagctg tgaaagctgg ttctttgttt
    16801 cgctaatgtg ctgctcaatt ctggaacgta actcaggata gttttctatc cggctggcca
    16861 tagactcaag cattttttca gcttgttttt ccatcgcatg tgca
//
LOCUS       DBFIFS010000087        16895 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.087, whole genome shotgun sequence.
ACCESSION   DBFIFS010000087 DBFIFS010000000
VERSION     DBFIFS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.087"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1390
                     /locus_tag="N7A07_002542"
     CDS             <1..1390
                     /locus_tag="N7A07_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2477183.1"
                     /translation="DAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRA
                     NKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKK
                     IMQAANISQPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQK
                     AEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFD
                     GENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTS
                     RAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDTM
                     KWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRRS
                     YERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSA
                     IWQKLIPSQADS"
     gene            1463..1714
                     /locus_tag="N7A07_002543"
     CDS             1463..1714
                     /locus_tag="N7A07_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU2477184.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1749..1936
                     /locus_tag="N7A07_002544"
                     /pseudo
     CDS             1749..1936
                     /locus_tag="N7A07_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(2411..2497)
                     /locus_tag="N7A07_002545"
                     /pseudo
     CDS             complement(2411..2497)
                     /locus_tag="N7A07_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2703..3029)
                     /gene="ynfA"
                     /locus_tag="N7A07_002546"
     CDS             complement(2703..3029)
                     /gene="ynfA"
                     /locus_tag="N7A07_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU2477185.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            3164..3505
                     /gene="ynfB"
                     /locus_tag="N7A07_002547"
     CDS             3164..3505
                     /gene="ynfB"
                     /locus_tag="N7A07_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU2477186.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3540..4100
                     /gene="speG"
                     /locus_tag="N7A07_002548"
     CDS             3540..4100
                     /gene="speG"
                     /locus_tag="N7A07_002548"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU2477187.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(4103..4813)
                     /gene="ynfC"
                     /locus_tag="N7A07_002549"
     CDS             complement(4103..4813)
                     /gene="ynfC"
                     /locus_tag="N7A07_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU2477188.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4921..5226
                     /gene="ynfD"
                     /locus_tag="N7A07_002550"
     CDS             4921..5226
                     /gene="ynfD"
                     /locus_tag="N7A07_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU2477189.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5425..7851
                     /gene="ynfE"
                     /locus_tag="N7A07_002551"
     CDS             5425..7851
                     /gene="ynfE"
                     /locus_tag="N7A07_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGU2477190.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7912..10335
                     /gene="ynfF"
                     /locus_tag="N7A07_002552"
     CDS             7912..10335
                     /gene="ynfF"
                     /locus_tag="N7A07_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU2477191.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            10346..10963
                     /gene="ynfG"
                     /locus_tag="N7A07_002553"
     CDS             10346..10963
                     /gene="ynfG"
                     /locus_tag="N7A07_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU2477192.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10965..11819
                     /gene="ynfH"
                     /locus_tag="N7A07_002554"
     CDS             10965..11819
                     /gene="ynfH"
                     /locus_tag="N7A07_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU2477193.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11862..12476
                     /gene="dmsD"
                     /locus_tag="N7A07_002555"
     CDS             11862..12476
                     /gene="dmsD"
                     /locus_tag="N7A07_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU2477194.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12634..13926
                     /gene="clcB"
                     /locus_tag="N7A07_002556"
     CDS             12634..13926
                     /gene="clcB"
                     /locus_tag="N7A07_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU2477195.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13879..14574)
                     /gene="bioD"
                     /locus_tag="N7A07_002557"
     CDS             complement(13879..14574)
                     /gene="bioD"
                     /locus_tag="N7A07_002557"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU2477196.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14699..15919)
                     /gene="mlc"
                     /locus_tag="N7A07_002558"
                     /gene_synonym="dgsA"
     CDS             complement(14699..15919)
                     /gene="mlc"
                     /locus_tag="N7A07_002558"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU2477197.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(16054..>16895)
                     /gene="ynfL"
                     /locus_tag="N7A07_002559"
     CDS             complement(16054..>16895)
                     /gene="ynfL"
                     /locus_tag="N7A07_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2477198.1"
                     /translation="HFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAA
                     GKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
                     PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDHPL
                     ANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLV
                     SAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLN
                     ALR"
BASE COUNT         4414 a         4245 c         4169 g         4067 t
ORIGIN      
        1 tgatgcagtg gaaacggcaa ttccagacaa cgaaaaaacc gaatgcaaag tggaagtcga
       61 accatctgta gaacgtgagg ggccgttcta cttcctcttc accgacaagg atggcgaaaa
      121 atacggtcgc gcaaacaaac tttctggtct ggataaggcg ctggctgccg gggctactga
      181 aatcacaaaa gaagaatatt ttgcccgaaa aaatggcaca tacacaggct taccgcaaaa
      241 tgcaaatacc gcacaaaatt ctgaacaacc agaaccggta aaagttaccg ctgacgaagt
      301 aaagaaaatt atgcaggcag ccaatatcag ccagcctgac gccgaggaac tgcttgcagt
      361 atcacgtggt gaatttgttg aagggattag cgacccgaat gatccgaaat gggttaaggg
      421 gatccagacc cgcgattctg tgaaccagaa ccagcaagaa acggaacaga acgaccagaa
      481 agcggaacaa aacagcccaa atacgcaaca aaacgagcca gaaacgaaac aacctgaacc
      541 agtagtgcaa caggaaccgg aaaagatctg caccgcctgc ggtcagagcg gtggcggcaa
      601 ctgccctgat tgtggtgcgg tgatgggtga cgcaacatac caggaaatat tcgatggaga
      661 gaatcagcct gaagttcagg aaaatgatcc ggaggaaatg gaaggtactg cacatcagca
      721 caaggagaac actggcggca atcagcatca tgccagcgat agtgaaactg gcgaggcgtc
      781 agatccctta attaaggcga acggtcatca taatctcaca tccaccagca gagcggggat
      841 tcatctgatg atcgatcttg aaaccatggg aaaaaatccc gatgccccga ttatctcaat
      901 aggtgcaata tttttcgatc cgcaaaccgg agatatggga ccggaattta gtaagactat
      961 cgatctggat actgctggcg gagtcattga tcgggacacc atgaaatggt ggcttaaaca
     1021 atcacgcgaa gcgcaatctg ccattatgac cgatgaaatc ccgttagatg atgcactgtt
     1081 acaattgcgg gaatttatcg acgaaaactc cggtgaattt tttgttcatg tctggggaaa
     1141 tggagccaac ttcgacaaca cgattttgcg ccgttcatac gaacggcagg ggagcccctg
     1201 cccgtggcgt tactacaacg atcgcgatgt acgcacaatc gttgagctgg ggaaagccat
     1261 agacttcgat gccagaacgg ctattccatt cgaaggtgag cgccataatg cacttgatga
     1321 cgcccgttac caggcaaaat acgtttcagc tatctggcaa aaactgatcc cgagtcaggc
     1381 tgattcttaa tgttcaactg tcgccggttg tgactggtat tctgcaaccg gcgctcgtct
     1441 gatgtaagag ataaagaaat cgatgagcga agtaatcatg attgtctctc ccggcaaatg
     1501 ggtatccgaa gagcagttaa ttgcgctgaa aggaataaaa aaaggtacgt taaaaaaggc
     1561 ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg acggtatgcc
     1621 atgggataac agtccatgct tttacaacct ggaagaaatt gatcgctgga ttgagcgcca
     1681 ggcatcagca agaccaagac gtcatcttac ttgactaaaa gccacactaa ctaatgagag
     1741 aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta cgtatctggt
     1801 ttgtttataa aggcgtaaga gtcagggaaa acctggggtt cctgacacag caaaaaacag
     1861 gcgcgttgca ggtgagctac gcgcctctgt ttgttacgca ataaaaactg gtgttttcga
     1921 ctatgcaaaa cagtttccct cctcacgcaa tctggaaaaa tttggtgagg cccgacaaga
     1981 tttaaccata aaagaactgg ctgaaaaatt tctggcactg aaagaaactg aagtcgcaaa
     2041 aacatcactc aacacatacc gtgccgtcat caaaaatatc ctgagcataa tcggtgaaaa
     2101 aaatcttgcc tcatcgatta ataaagaaaa attactggag gttcgtaaag agctactgac
     2161 tggataccag atccccaaaa gtaactatat tgttacacaa ccagggagat cggctgtaac
     2221 tgtaaataat tacatgacaa atcttaacgc cgtgttccag tttggtgttg ataacggtta
     2281 cctggcagat aacccgttta aggggatctc gccattaaag gaatcaagaa ccattccgga
     2341 tcctctttcg cgggaggaat tcatccgtct tatcgatgcg tgcagaaatc agcaagccaa
     2401 aaatttatgg tgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg
     2461 gacaggtaac aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt
     2521 cgtgatgcat gaaatctacg caactgagct actaccatat aagtatgaag atcgaaaaaa
     2581 gccggagtga tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa
     2641 atagaatacc ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt
     2701 tcctacgtgc gcccccagcc cacaacaatg atcaacatgc cgcaaagcgc aatcaacggc
     2761 cccgtccagt cataaagagt cagtttcacg ccatccacaa cgcgcagcca catcaacgcc
     2821 gtgcagacat aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac
     2881 gttaacaacc agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg
     2941 gcgtttcgtt ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta
     3001 gcaaaaaata gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta
     3061 tagggcgaca taatatcatc aatataaaca ccctcctggt acgttttgcg ttcgcagtgg
     3121 atggtgtaga atcatcttta atcattcata cagggaatga attatgaaaa tcactctcag
     3181 caaacgaatc gacctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt
     3241 tcatgccgaa actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca
     3301 cgccgctatg gaaaaagagc aatggaatga cacgggcaat ctgcgccaga aagtgaataa
     3361 acgcactgaa aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgcga
     3421 gcaaagcgcc aacatcaatg cctactggga gccaaatact ttgcgctgcc tggaccgtcg
     3481 aactggccgc gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta
     3541 tgccaagcgc ccacagtgtt aagctacgcc cgctggagcg tgaagattta cgctatgtac
     3601 atcaactcga caataacgcc agtgtgatgc gttactggtt tgaggaaccc tacgaagcct
     3661 ttgttgaact ctctgatctg tatgataagc atattcacga tcagagcgaa cgacgctttg
     3721 tggtggaatg tgacggcgaa aaagccggtc tggtggagtt ggtggaaatt aaccatgtac
     3781 atcgccgcgc agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa
     3841 cccgtgccgc caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt
     3901 atctgatcgt tgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggctttt
     3961 cggttgaagg tgaattgatg cacgagttct ttattaatgg tcaatatcgt aatgccattc
     4021 gcatgtgtat attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc
     4081 tgaagccgac cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc
     4141 gttcgacggc gggttttact ccttcatcaa caatgatcag ctgacagccc accggattgg
     4201 cgtgactgtc atattcacag ctctgtttta cattaccaac tcgttgatta ttcagtaaag
     4261 taaccgctgt gtaatctaat tttttgatcg ggtccgttga tggcgtggcg ctgacagata
     4321 atgttttgtc gttacttttc gtggttttac ccagtggata accctgatca tcatagcgat
     4381 attccatctg catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc
     4441 aactcacccc ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct
     4501 tctccagcgt ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat
     4561 tattttccag atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtccccg
     4621 aaacacgttt cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg
     4681 gaccacgcag cggatcaaaa tcgaattcat tagagaaact ggccatctca ggagtaaatg
     4741 aaagtgttac ctctgtgcgg tcacatcctg tgagtaatat cgcgagtaag catggcagta
     4801 atttgtattt cacatcagtc accagagagt agagatgatt ctcaatcata gtagcaaata
     4861 cagtacttta cacgtgaaat gctatgctta aagaagttat cttcgcgtta ggagcttatg
     4921 atgaaactct caacctgtta tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc
     4981 gccgcgccgg gatcctgtga gcgcatacaa agcgatatat cacagcgcat tatcaataat
     5041 ggcgtacccg aaagcagctt cacgttaagt attgtaccca atgaccaagt tgatcagcct
     5101 gattcccagg tcgtcgggca ttgcgctaat gatacgcata aaattctcta cacccgcacc
     5161 accagcggta acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg
     5221 cagtaataca ttatcgacta cacgccggat acgacacatc cggcgatttg gacatacaac
     5281 ataatcccac catattactc atacccttct attgatatgg attaataatt attaacccaa
     5341 aatgggtaga ctccctctat tgttagcgcg ctaaatattc aatatataaa cttttatata
     5401 acgataaaga acagggagtg agttatgtcc aaaaatgaac gaatggtggg catcagccgc
     5461 agaacactcg ttaaatctac cgcgataggt tctctggcgc tggctgcagg cggtttttct
     5521 ttgccgttca ccctgcgcag tgcagcggca gcagtacaac aggccagcga acaagtggtc
     5581 tggggtgcct gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat
     5641 aatgaagtgg cctgggtgga aactgacaac accggcagcg atgagtatgg caaccatcag
     5701 gtacgcgcct gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg
     5761 aattacccaa tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg
     5821 gatgaagccc tggatactat cgccagtagc ctgaagaaaa ccgtcgaaca atatggcaat
     5881 gaggctgtat atattcagta ctcttcgggg atcgttggcg gcaatataac ccgctcttcg
     5941 ccatcagcct cggcggtcaa acgcctgatg aactgctacg gcggttcact caaccagtat
     6001 ggctcctaca gcactgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat
     6061 ggcaacagca ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg
     6121 gcagaaaccc gcatgagcgg cggtggcatc acttatcttc ttgaaaaatc ccgcgaaaaa
     6181 tcgaacgcaa aaatgattgt tatcgatccg cgatataccg atacggctgc tggtcgtgaa
     6241 gatgaatggc ttcctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg
     6301 gtattgatta acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat
     6361 gacgaaaaaa ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt
     6421 ggtgaaggtg acgataaaac agcgaaaacg ccgcagtggg cttcgcaaat taccggtatc
     6481 ccggtggacc gtatcatcaa actggcgcgt gaaattggca cagcaaaacc cgcttatatc
     6541 tgccagggct gggggccgca acgccaggca aacggcgagc tgaccgcacg cgctattgct
     6601 atgttgccta ttctgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa
     6661 tcgacctata ccattaccat agagcgcctg ccggtgctgg ataatccggt caaaacgtca
     6721 atttcctgct tcagctggac agatgctatc gatcatggcc cacaaatgac ggcaatccgc
     6781 gacggtgtcc ggggcaaaga taaactggat gtgcccatta agttcatctg gaactacgcg
     6841 ggaaataccc tggttaatca gcattctgac atcaacaaaa cgcatgaaat tctgcaggac
     6901 gaatcgaaat gcgaaatgat tgtggtcatc gaaaacttta tgacctcatc ggcaaaatat
     6961 gccgacattc tgctgccaga cctgatgact gttgagcagg aagatattat tcctaacgac
     7021 tacgccggta acatgggata tctcattttc ctccagcctg tcaccagcga aaaattcgaa
     7081 cgcaaaccga tttactggat cctgagtgaa gtcgcgaaac gtcttggacc tgacgtctat
     7141 caaaagttca ctgaaggtcg cacgcaggaa caatggttac aacatctgta cgccaaaatg
     7201 cttgccaaag atccggcgtt accgtcttac gacgaactga aaaaaatggg tatctataag
     7261 cgtaaagatc ccaatggtca ttttgtcgcc tacaaagcat ttcgtgacga ccccgaggca
     7321 aatccactta aaacaccttc cggtaagatt gaaatttatt ccagcaagct ggcggaaatt
     7381 gcccgtacct gggaactgga aaaagatgaa gtgataagcc cactgccagt ttatgcctca
     7441 acctttgaag gctgggactc ccctgaacgt agtacctttc cactgcaact gtttggtttc
     7501 cattacaaat cccgtactca ctcgacctac ggcaacattg atgtcctgaa ggctgcctgc
     7561 cgtcaggagg tgtggatcaa ccctatagac gcacagaaac gtgggattgc caacggcgat
     7621 atggtgcggg tgtataacca tcgtggcgaa gttcggctac cagccaaagt gacaccacgc
     7681 attctccctg gggttagcgc tatgggccag ggagcctggc acgaggccaa tatgtctggc
     7741 gataaaatcg accatggcgg atgtgtgaat acgctaacca ctctgcgccc ttcaccactg
     7801 gcgaagggaa acccgcagca cacgaatctg gtcgagatcg aaaaaatata acccactaca
     7861 accataaatt ctggcatgac attttgtttg aaaagcaata agtgagtaat gatgaaaatc
     7921 cataccacag aggcgctcat gaaggctgaa atcagccgta gaagtctgat gaaaacctcc
     7981 gcacttggca gtcttgcgct ggcaagcagt gctttcactc tgccattttc ccaaatggtc
     8041 cgagcggcag aggcaccggt agaagagaaa gcggtctgga gttcctgcac cgttaactgc
     8101 gggagccgct gtctgttacg tttgcatgtg aaagatgaca ccgtgtactg ggtggagtct
     8161 gatacgacag gtgacgacgt ctacggtaat catcaggttc gtgcgtgttt acgcgggcgc
     8221 tctatccgcc gacggatgaa tcatcctgat aggttgaaat atcccatgaa gcgcgtcggc
     8281 aagcgcggtg aaggtaaatt tgaacggata agttgggacg aagccctgga taccatcagt
     8341 gataatcttc ggcggatcct gaaagattac ggcaatgagg ctgtacatgt cctgtacgga
     8401 acaggcgtag atggcggaaa catcaccaac tcaaacgtcc cgtaccgtct gatgaactct
     8461 tgcggtggtt ttctcagtcg ctatggcagc tacagtaccg cacagatcag tgccgcaatg
     8521 agttatatgt tcggtgccaa tgatggcaac agcccggatg atatcgccaa tacgaaactg
     8581 gtcgttatgt tcggaaataa cccggcagaa acgcggatga gcggtggtgg tgtcacttac
     8641 tacgtcgagc aagcccgcga acgttcaaac gcacgcatga tcgtcatcga tccacgttat
     8701 aacgacacag ctgccgggcg tgaagatgaa tggctgccca ttcgccctgg caccgatggc
     8761 gcactggcct gtgcgattgc ctgggtactg attagtgaaa acatggtcga tcagccattt
     8821 ctcgacaaat attgtgttgg ttacgatgaa aaaacgctgc ccgccaacgc accacgtaac
     8881 gcgcattata aagcctatat tctgggcgaa gggcctgacg gcatagctaa aacgccggaa
     8941 tgggcagcaa aaatcaccag catcccggca gaaaaaatta tccagttggc acgagagatc
     9001 ggttcagcaa aacctgctta tatttgtcag ggttgggggc cacaacgaca ttccaacggc
     9061 gagcaaacat cccgcgctat tgccatgctt tccgttctca ccggtaacgt cggcataaac
     9121 ggcggcaact caggtgtacg cgaaggtagc tgggatctgg gggtagaatg gttcccgatg
     9181 ctcgagaatc ctgttaaaac gcagatttcc gtctttacat ggacagatgc catcgaccat
     9241 ggtacggaaa tgaccgcgac ccgtgatggt gttcgtggaa aagaaaaact ggatgtcccc
     9301 atcaagtttt tatggtgcta cgccagtaac acattgatca atcaacatgg cgacatcaat
     9361 cacaccaatg aggtgcttca ggatgacagc aagtgcgaga tgattgttgg cattgaccac
     9421 ttcatgacgg cctcggctaa gtattgcgat atcctgttgc ccgacctgat gccaacagag
     9481 caagaagacc ttatctctca tgaatctgca gggaatatgg gctatgtgat cctcgcccaa
     9541 cccgcaacct cagcaaaatt tgaacgcaaa cccatctact ggatgctgag tgaagtcgcc
     9601 aaacgcttag gaccggacgt ttatcaaacc tttactgaag gtcgcagtca gcatgaatgg
     9661 atcaaatatc tccatgcgaa aacgaaggaa cgtaaccctg agatgcccga ctacgaggag
     9721 atgaaaacga ccgggatctt taagaaaaaa tgcccggaag aacactacgt cgctttccgc
     9781 gcattccgtg aagatccaca ggcaaacccg ttgaaaacac cttcggggaa aatcgaaatt
     9841 tattctgaac gactggcgaa gattgcagat acctgggaat tgaaaaaaga tgaaattatt
     9901 catccccttc ctgcgtatac cccaggtttt gatggctggg acgatcccct gcggaaaacc
     9961 tatccactgc agttaacggg cttccactac aaagcgcgta cccactccag ctacggcaat
    10021 attgatgtgt tacagcaggc ctgcccacaa gaggtgtgga tcaaccccat tgatgctcag
    10081 gctcgcggta tccgtcatgg cgataccgtg cgggtattta acaataatgg agaaatgctg
    10141 attgccgcaa aagtgactcc gcgtattctg cctggcgtta ccgccatcgg gcaaggtgcg
    10201 tggcttaaag cggatatgtt tggtgaccgg gtcgatcacg gcggcagtat caatattctg
    10261 acctctcacc gcccttcacc gctggcaaag ggaaacccgt cgcacagcaa tcttgtccag
    10321 atcgaaaagg tttaaggagt agcccatgac cacacaatat ggatttttta tcgattccag
    10381 ccgctgtact ggctgtaaaa cctgcgaact ggcgtgcaaa gattttaaag atcttggccc
    10441 ggaagtcagt ttccgccgta tttatgaata tgctggcggc gactggcagg aagataatgg
    10501 cgtctggcac cagaacgtgt tcgcttatta tctctccatc tcctgtaacc attgcgatga
    10561 ccccgcatgt acaaaagtct gccccagcgg cgcaatgcat aagcgtgaag atggctttgt
    10621 ggtggttgat gaagatgttt gtatcggctg ccgctactgc cacatggctt gtccgtacgg
    10681 cgcgccacag tacaatgctg aaaaagggca catgacgaag tgcgatggtt gttattcgcg
    10741 cgtcgccgag gggaaacaac ccatatgtgt cgaatcctgc ccgctgcgtg cacttgagtt
    10801 cggtccaatt gaagagctgc gccagaagca cggcactctt gctgccgtcg cgccactgcc
    10861 gcgcgcgcat ttcacaaaac ccaatatcgt tatcaaacct aacgccaaca gccgcccgac
    10921 cggcgatacc actggttatc tggctaatcc ggaggaagtg taaaatggga aatggatggc
    10981 atgaatggcc actggttatc tttaccgtat tgggtcaatg cgtagtaggc gcgctgattg
    11041 ttagcggaat tggctggttc gccgccaaaa ctgacgcaga ccgtcagcac attgttcgtg
    11101 gcatgttttt cctctggctg ttgatgggca tcgggtttat tgcctctgtc atgcacctgg
    11161 gatcgccact gcgggcgttt aactcgctga atcgaatcgg agcttctggt ctgagtaacg
    11221 agattgctgc cggttccata ttttttgccg ttggcggact atggtggctg gtggcggtca
    11281 tcggtaaaat gccgcaagcg ttggggaaac tctggttact tgtcagcatg gcgctcggcg
    11341 tcattttcgt ctggatgatg acctgcgtgt atcaaatcga cactgtgcca acctggcata
    11401 acggttacac tacgctggct ttcttcgtga cggtattgtt gagtggtcca atacttgctg
    11461 ccgcaattct gcgggcagcc cgcgttacat ttaataccac accatttgcc atcattagcg
    11521 ttctggcgct gatcgcttgc gccggggtga ttgtgctgca aggtttgtct ctgtcgtcta
    11581 ttcactcatc cgtgcaacaa gccagcgcac tggtaccaga ttacgcctcc ttacaggtct
    11641 ggcgtgtggt attgttatgc gcaggtctag gatgctggct ttgtccgctc atacgtcgcc
    11701 gtgaacctca cgttgccgga cttattctgg ggttaatcct cattcttggc ggtgagatga
    11761 tcgggcgtgt gctcttttat ggcctgcaca tgacggtagg aatggctatt gcaggttaac
    11821 aaattggtgc ggggcaaccc gcacatttca ggatgttagg aatgacccat ttttcacagc
    11881 aagataattt ttctgtcgcg gcgcgcgtgt tgggcgcgct gttttattac gctccagaga
    11941 gtgctgaagc cgcacctctg gttgcggtac tcaccagtga cggctgggaa actcagtggc
    12001 ctctaccaga agcgtcatta gcgccgctgg tgacggcttt tcagacacag tgtgaagaga
    12061 ctcacgccca ggcctggcag cgtttgtttg tcggcccgtg ggcactgccg tctccgccgt
    12121 gggggtcggt ctggctggat cgcgaatctg tgctgtttgg cgattcaaca ttggcacttc
    12181 gtcagtggat gcgcgagaaa ggcattcagt ttgaaatgaa gcaaaacgaa ccggaagatc
    12241 attttggttc actgctgttg atggctgcgt ggttggcgga gaatggtcgc cagacagaat
    12301 gcgaagaatt gctggcatgg cacctttttc cctggtcaac acgttttctt gacgttttta
    12361 tcgaaaaagc agaacaccct ttctaccgtg cactgggtga actggctcgc ttaacgctgg
    12421 cgcaatggca atcacaactg ttaattcctg tcgcggttaa accactcttt cgataggatc
    12481 accgtaatat tgccggatgg tgacgattac gcgcgattcg gcaacaatac gttacagtgt
    12541 cacgtttttt gtctcttaaa gcacgcactg cttttgcagc tggcctcttt tgccgcaaaa
    12601 tagtcgcccg tgtttcattg cccatttctg ctcatgcatc atctacacac ctatccggat
    12661 ctgcgcacta tgttccgccg tctgcttatc gcaacagtcg tcggtattct cgcggccttt
    12721 gccgttgccg ggtttcgtca tgcgatgctg ctactggagt ggttgttcct caataatgac
    12781 tccggcagtc tggtcaatgc agcgacaaac ctttccccct ggcgacggct gctaactccg
    12841 gcgctcggcg gactggcggc gggtttgttg ttgatgggct ggcagaaatt tacccagcaa
    12901 cgccctcatg cgccgaccga ttacatggaa gcgttgcaaa ccgatggtca gtttgattac
    12961 gcagcaagcc tggttaaatc gcttgcctct ctgctggtag taaccagcgg cagtgcaatt
    13021 ggtcgcgaag gtgcgatgat tcttttagct gcccttgccg cctcctgttt tgcccaacgt
    13081 tttacgccac gccaggagtg gaaattatgg atcgcctgtg gggccgcggc gggaatggct
    13141 gcggcctatc gtgccccgct tgctggcagt ttatttatag ccgaagtgct gtttggcact
    13201 atgatgttgg cctctctcgg cccggtgatt atttccgccg tcgtggcatt gctggttagc
    13261 aatctgatta atcatagcga cgcgttactc tacagcgtac aactctcagt gacggttcag
    13321 gctcgtgact atgcgctgat tatcagtaca ggtgtgctgg caggtctgtg cggaccactg
    13381 ttgttaacgt taatgaacgc ctgtcatcgt ggatttgtga gtctcaaact tgcgccgccc
    13441 tggcaactgg cactaggcgg gttgatcgtg ggtctgcttt ccctgttcac acctgcagtg
    13501 tggggcaacg gctatagcac cgtacaatcc tttttaaccg ccccgccact gttaatgatc
    13561 attgccggga tcttcctttg taaactgtgt gccgtgctgg cgagtagcgg ttccggcgca
    13621 cccggtggtg tctttacacc gacgctattt atcggtcttg ccattggcat gttgtatggt
    13681 cgtagtctgg gattatggtt ccctgatggc gaagaaatta cgcttttact cggattgacc
    13741 gggatggcga cactgttggc ggcaaccacg cacgcgccga ttatgtcgac gttgatgata
    13801 tgtgaaatga ccggggagta tcagctactc cccggtttat tgattgcctg cgtaattgcg
    13861 tcggtaatct cgcggacgtt acaccgtgac tctatctacc gccagcacac tgcgcagcat
    13921 agctaagcgg atgtattgcc ccagttcacg ctgttcggcg cgcggcagat aaggcagttc
    13981 accaatgagc ggtgccggaa gttttttacc gagcacatca atgatttccg cataatgcgc
    14041 cagtcctggg ttgattcggt tagccaccca gccaatgagc ggcagcccgt cgttggcaat
    14101 cgcctgagct gttagcagtg catggttaat gcaaccttcc tgaataccga caaccatcaa
    14161 caccggcagt tgttcctgca ccacccattc agagagtgga cgcaaatcat tcatcagact
    14221 acgccagccg ccagtccctt ctaccacgac atgatcgact ttttcggtca ggtttgccag
    14281 gccgtttgaa atgagggtgt aattgattgg gcaactgtgc gccacgctac tttcttcttc
    14341 gcttaacgcg ataggattaa ctgcttcata aggcagttcg atggttgaaa cactctgcaa
    14401 caccagggca tctttattac gcagcccttc gggtgtctct ttgctcccct tcgctacggg
    14461 tttatatccc gcaaccgttt ttccctggga ggctaacgct tgtagcaatg cgcgggaaac
    14521 caccgttttc cctacagaag tgtctgtacc ggtaataaag aaacgcttca gcatcactaa
    14581 ctccaccgtt atgcttcaca aatataaacc aggaaaataa ttaaccttga aagtctaagt
    14641 tatgctttcc tggcccaaat tgagatagcg caaattttgg tagaacagtt aaaaaatgtt
    14701 aaccctgcaa cagacgaatc aacaaagaac cgttatacat cgcgtctttt accagcgcag
    14761 cccctgccat cgtgccctgg ttagaaaact gagtactctc aacgctaatg tgctgactat
    14821 acgcaggaag ggcctgctga cggatgctat ctgagatgac cgggaagagg atatctgccg
    14881 ctttacttaa cggtgagcca atcagtattt tttgtgggtt aaataaattc accatgatgg
    14941 caagaatgcg ccctacatgc gcgcccaccc cggtaatgat gtcttttgcc agcagatcgc
    15001 cgcgcaatgc cgcctgacac aatgagtcca cggttaacgg ttgcccatgt aacatcgagc
    15061 tcatggattg attgagacgc agctgtgcca gctcaagaat actgtccaca ctggcgatgg
    15121 tttcgaggca gccgtggttc ccgcaataac agcgtttacc atatgggtct acctgtgtgt
    15181 gacctatttc cacgaggcta ctgctgcctg cgtgtagcag atgaccatcg gtaatgacgc
    15241 ccgcccccac gttgtgatcg ataaccacct gaatcacatc gcgcgccccg cgtgaggcac
    15301 caaacaaggc ctctgccatc gtccatgcgc tgatatcatg ctgaatataa accggaacgc
    15361 cggtatgctg ctccagagcc tcgccgagcg gcatctcttt tacatcctcg tagaacggca
    15421 tgcgatgtac aatgccattt tccgtatcaa taattcccgg cagggttatg gcaatcgaag
    15481 ttagacgctc aagttttttc tggtggcgga taaaaaactg atcgatatgg gaaatgatac
    15541 gatccagcaa tggcgagtca tcttttaacg ccagttcctg cgactcttcc accaccagtt
    15601 tgctgctcag atcgcgcaga gcaaggaaaa tctccccgcg actaatgcgc agagaaagat
    15661 agtgccaggc ttcagtttca accaccagcc ccaccgccgg acggccacgg ttccccgctt
    15721 ctttgatttc cagctcttgc accaggtgtg cttcgagcat ctcacggaca attttagtga
    15781 tactggcagg agccagctgc gccagacggg aaagatcgat acgcgagact ggaccaagct
    15841 gatcaatcag gcgataaacc gcgcccgcgt tggtctgctt tatttgatca atgtgcccag
    15901 gctggttttc agcaaccacc gcatactccc tatattttcg cgctccgaaa taatctgtag
    15961 gctatggtga agcacttcaa tacgtgtcgt caaattttta cttaggcatg tgattaacag
    16021 cacatttttc gggctttttc gctgaaattt ccctcacctg agagcattca gcagatgaat
    16081 acgaaagtta cgcgcagctg gactttgttc atgatgtttc ggccagacca accacatttc
    16141 agaaaccgca tcctcttcag caatcggcac ccagcgcatt tcgttgagct gaacacgttt
    16201 aaatgacgca ggcaaaattg aaacacccag accggcggaa accagaccga tgatggtcat
    16261 tgcctcgccc acctcctgag tgatgacggg cgtcaaatgg taacgtcgca tcagcccgag
    16321 aatatcgtca tacagccctg tcccgacgtg cggatcaaaa aagacaaagg gttctttcgc
    16381 cagttcagcc agcgttacat tcgggttatt tgccagggga tgatcgtgcg ggatcatcgc
    16441 cataagcggt tcatggacga tgactgcgtg ttcaagcgtc tccggtaacg ctgtgttacg
    16501 cagcaatccc atatccagcg ttccttcaat gagcggagcg atttgctcgc gagtgttcat
    16561 ttcgcgggtt tgtaaatgga catcgggata atctcggcga aacagcgata acgtatcgga
    16621 caccgcccga ataaaaggag ccgacgaagt aaaaccaatg cgcaactccc ccgcttcacc
    16681 ctgatgcagt ctttcagcgc gagcggcagc gtcatccacc atagacagga tttgccgact
    16741 atctgcaaga aactgttttc ctgctgccgt cagcaataca ctgcgattgg ttcgtgccag
    16801 cagtcgggca ccaatttgtt gctccagcgc ctgaatctgc tgacttagcg gcggttgcga
    16861 aatattcagg cgggcagcgg cgcgcccgaa atgca
//
LOCUS       DBFIFS010000088        16675 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.088, whole genome shotgun sequence.
ACCESSION   DBFIFS010000088 DBFIFS010000000
VERSION     DBFIFS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.088"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..548
                     /locus_tag="N7A07_002560"
     CDS             <1..548
                     /locus_tag="N7A07_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU2477199.1"
                     /translation="KETTSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQV
                     GQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVP
                     VALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFE
                     NIKQVIDDMATDATMFDVNKK"
     gene            complement(583..1756)
                     /gene="ydcO"
                     /locus_tag="N7A07_002561"
                     /pseudo
     CDS             complement(583..1756)
                     /gene="ydcO"
                     /locus_tag="N7A07_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
     gene            1848..2384
                     /gene="sutR"
                     /locus_tag="N7A07_002562"
     CDS             1848..2384
                     /gene="sutR"
                     /locus_tag="N7A07_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU2477200.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2457..4418
                     /gene="rlhA"
                     /locus_tag="N7A07_002563"
     CDS             2457..4418
                     /gene="rlhA"
                     /locus_tag="N7A07_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU2477201.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4510..4740)
                     /gene="yncJ"
                     /locus_tag="N7A07_002564"
     CDS             complement(4510..4740)
                     /gene="yncJ"
                     /locus_tag="N7A07_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU2477202.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4962..5138
                     /gene="hicA"
                     /locus_tag="N7A07_002565"
     CDS             4962..5138
                     /gene="hicA"
                     /locus_tag="N7A07_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU2477203.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5184..5600
                     /gene="hicB"
                     /locus_tag="N7A07_002566"
     CDS             5184..5600
                     /gene="hicB"
                     /locus_tag="N7A07_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU2477204.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5679..7085
                     /gene="ydcR"
                     /locus_tag="N7A07_002567"
     CDS             5679..7085
                     /gene="ydcR"
                     /locus_tag="N7A07_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2477205.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            7330..8475
                     /gene="ydcS"
                     /locus_tag="N7A07_002568"
     CDS             7330..8475
                     /gene="ydcS"
                     /locus_tag="N7A07_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU2477206.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8493..9506
                     /gene="ydcT"
                     /locus_tag="N7A07_002569"
     CDS             8493..9506
                     /gene="ydcT"
                     /locus_tag="N7A07_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2477207.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9507..10448
                     /gene="ydcU"
                     /locus_tag="N7A07_002570"
     CDS             9507..10448
                     /gene="ydcU"
                     /locus_tag="N7A07_002570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477208.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10438..11232
                     /gene="ydcV"
                     /locus_tag="N7A07_002571"
     CDS             10438..11232
                     /gene="ydcV"
                     /locus_tag="N7A07_002571"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477209.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLESGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11254..12678
                     /gene="patD"
                     /locus_tag="N7A07_002572"
     CDS             11254..12678
                     /gene="patD"
                     /locus_tag="N7A07_002572"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU2477210.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12775..12870)
                     /gene="yncL"
                     /locus_tag="N7A07_002573"
     CDS             complement(12775..12870)
                     /gene="yncL"
                     /locus_tag="N7A07_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU2477211.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13065..13238
                     /gene="ortT"
                     /locus_tag="N7A07_002574"
     CDS             13065..13238
                     /gene="ortT"
                     /locus_tag="N7A07_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU2477212.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13324..13557
                     /gene="ydcY"
                     /locus_tag="N7A07_002575"
     CDS             13324..13557
                     /gene="ydcY"
                     /locus_tag="N7A07_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU2477213.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13558..14007)
                     /gene="ydcZ"
                     /locus_tag="N7A07_002576"
     CDS             complement(13558..14007)
                     /gene="ydcZ"
                     /locus_tag="N7A07_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2477214.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(14004..14522)
                     /gene="mddA"
                     /locus_tag="N7A07_002577"
     CDS             complement(14004..14522)
                     /gene="mddA"
                     /locus_tag="N7A07_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU2477215.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14703..15740
                     /gene="curA"
                     /locus_tag="N7A07_002578"
     CDS             14703..15740
                     /gene="curA"
                     /locus_tag="N7A07_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU2477216.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15938..16603
                     /gene="mcbR"
                     /locus_tag="N7A07_002579"
     CDS             15938..16603
                     /gene="mcbR"
                     /locus_tag="N7A07_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU2477217.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT         4198 a         4035 c         4391 g         4051 t
ORIGIN      
        1 taaaagaaac aacctcggct acaggtaaac cggttttacg ttgggtagac ccgagttttg
       61 atcaaagcaa atatgacagc atcgtctgga acccaatcac ttattatccg gtaccgaaac
      121 cttcgaccca ggtagggcag aaagttctgg ataaaatttt gaactatacc aacaccgaaa
      181 tgaaagaagc gatagcgcag cgtaaaccac tggttaccac cgcggggccg cgtagtctga
      241 ttttccgtgg ggccattacc ggtgtagata ccagcaaaga agggctgcaa ttctatgaag
      301 tggttcctgt tgcattagtg gttgcgggga cgcaaatggc tacaggccac cgtaccatgg
      361 atactcgctt gtattttgaa ggtgagctga ttgatgcagc gactaataaa ccggttatca
      421 aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga aagtacgcca atggctttcg
      481 aaaatattaa acaagttatt gatgacatgg cgaccgatgc caccatgttt gacgttaaca
      541 aaaagtagtt ccagacgcgc catcgttaga tggcgctttt tattatcggt ttctgtcagc
      601 gattaaattc aacaccacgt aacaaacgcc tccggcaatt aatccccaaa acgcagaacc
      661 aatcccgacc agcgtcaatc cacttgccgt taccagaaat gccaccaccg ccgcgtcgcg
      721 ctcacgctca ttatgcagcg cctgatacaa actgccgcca atggtactca acagcgccag
      781 acctgccagc atctggatcc aacttacggg cagggcagcc atcatcccgg taatggcact
      841 accaaacaga cctgcgagca aatagaaaat gcctgcaacg gcagctgcca gccaacgttg
      901 atctttatcc ggatgcgctt ccgggctttg gcaaatagcc gcggtgattg ccgcaatacc
      961 gacggaataa acgccgaaag gggaaaaaac cagtgccagc aatccagtaa acacaatcaa
     1021 cggcgaaaca ggagccgaat acccggctgc tttcattgct gcgatacccg gtgcgttttg
     1081 cgatgccatc gtcaccagaa aaagggggga gtgcaacgct caggctgtga gcaaacgaaa
     1141 aatcaggggt aatataagtg gggagaacgg gtttaaagac aacatcagtt gtgacaacgt
     1201 caccttgcgc gataacgatc acgatcccaa taatcatcgc ggcaattacc gcatagcgcg
     1261 gcgcaacggc cttggttgcc agccatacca gcaacatact tccacacaac gtaaattgac
     1321 cgtccagact ggcaaacgcc tgtaaaccaa agcgtaataa aatcccggca agcattgctg
     1381 ccgcaagcga gtgcggaata atgcgcatca gacgagcaaa gagtcccgtt atgccgcaga
     1441 ggactattaa cgcgttggtg acaataaaaa cgccgatggc ttcgttaagt gttagtccct
     1501 gcaatccggt gaccaacaaa gccgcgccag gcgttgacca tgcggtgaga acaggtacgc
     1561 gataccataa tgtcagagtc agcgtactga cgcccattgc cagccccagc gccgtcatcc
     1621 agccagagat ttgtgcagtg gtggctccgg cgacaatcgc tgcttgccag attattgccg
     1681 ctgaactggc gtagccaatt aataccgcca gaaaccccgc cagtagcgtg ggtggaggaa
     1741 cagaaaacag acgcatagtc acctcgtgcg ttatagcgta caggcaccgt agcatttgtc
     1801 cgttataacg cacaagtgat aaacttccgt tttgccggag gagtcgcatg gaaaatctcg
     1861 ctcgcttttt atccaccaca cttaaacaac tacgtcagca gcgcggctgg agtctttcgc
     1921 gactggcaga agcgacgggc gtttctaaag caatgttagg acagattgag cgtaatgagt
     1981 ccagcccgac ggtcgcgaca ttatggaaaa ttgccactgg gttgaacgtg ccattttcga
     2041 catttatttc tccgccacag tccgctacgc cttcagttta tgatccacaa cagcaggcga
     2101 tggtgatcat ttcgctgttt ccttatgatc cacaactctg ctttgaacac ttctcgattc
     2161 agatggcacc cggtgcaata agcgaatcga cgccccatga gaagggggta atcgaacatg
     2221 tcgtcgtcat tgatggacaa cttgatctgt gcgttgatgg tgaatggcag acccttaatt
     2281 gtggggaagg cgttcgattt gctgcagacg tcacgcatat ctatcgtaat ggtggggagc
     2341 aaaccgtaca ttttcattcc ctcatccatt acccgcgcag ttaagcggga aaactatttc
     2401 gcaacgtcgc gcttgtggct aaaatagccg ccatttttca gctactggat aagaatgtga
     2461 ccgtatcttc tcatcgactt gaactgttaa gcccggcacg cgatgccgcc attgcccgcg
     2521 aagctatttt gcacggtgcc gatgctgttt atatcggcgg ccctggtttt ggtgcccgtc
     2581 ataatgccag taatagcctg aaagatattg ccgagctggt gccgtttgcc catcgttatg
     2641 gtgcaaaaat tttcgtcacg cttaacacca ttttgcatga tgatgagctg gaacccgcgc
     2701 aacggctgat tactgacctc taccagaccg gtgtcgatgc gctgattgtt caggatatgg
     2761 ggattctgga acttgatatt ccgccgattg aactgcacgc cagtacgcag tgcgacattc
     2821 gtacagtaga aaaagcgaag ttcctctctg atgttggctt cacgcagatt gtgctggcgc
     2881 gagagctgaa tcttgatcag atccgcgcga ttcaccaggc cacggacgcg accattgaat
     2941 tctttattca tggcgcactg tgcgtggcct attcgggtca gtgctacatt tctcatgcgc
     3001 aaacagggcg tagcgccaac cgtggcgatt gctcgcaagc gtgccgtttg ccatacacat
     3061 tgaaagacga tcaggggcgg gtggtttcct atgaaaaaca tctgctgtcg atgaaagata
     3121 acgatcagac tgccaacctc ggcgcgctga ttgatgctgg cgtacgttcc ttcaagattg
     3181 aagggcgtta caaagatatg agctacgtga agaatatcac cgcccattat cgccagatgc
     3241 ttgatgccat tattgaagaa cgtggcgatc tggcgcgcgc ttcatcaggt cgtactgaac
     3301 atttctttgt tccatcgacg gaaaagactt tccaccgtgg tagcacagat tattttgtga
     3361 atgcccgtaa aggcgatatt ggcgcgttcg attcgccgaa atttatcggc ctgccggtag
     3421 gtgaagtagt gaaagtggcg aaagatcatc tcgatgttgc cgttaccgag ccactggcaa
     3481 atggcgatgg cctgaacgtg ttgattaaac gtgaagtcgt cggttttcgt gccaatacgg
     3541 tcgagaaaac cggagaaaat cagtaccgcg tctggcccaa tgaaatgcca gcagatttgc
     3601 acaaaattcg cccgcatcac ccactaaacc gtaatcttga tcataactgg cagcaggcac
     3661 tgacaaaaac ctctagcgaa cgtcgggtgg cggtagacat tgaactgggc ggttggcagg
     3721 aacaattgat tctgactctc accagtgaag agggggtcag cataacgcat acgctggatg
     3781 ggcagttcga cgaagccaat aacgcagaaa aagcaatgaa caatctgaag gatggtctgg
     3841 caaaactggg gcaaaccctc tattacgccc gcgatgtgca aattaatttg ccgggggcgc
     3901 tgtttgtacc aaacagtctg ttaaaccagt tccgccgtga agctgccgac atgctggatg
     3961 ctgcgcgtct tgccagttac cagcgcggca gccgtaaacc ggttgctgat cctgcgccgg
     4021 tttatccgca aacacatctg agtttcctcg cgaacgttta caaccagaaa gcgcgtgaat
     4081 tttatcatcg ctatggtgtg cagctgattg acgcggcgta tgaggcacat gaagagaagg
     4141 gcgaagtccc ggtgatgatc accaagcatt gtctgcgctt tgcctttaat ctgtgcccga
     4201 aacaggcgaa aggcaatatc aaaagctgga aggcgacgcc aatgcaactg gttaacggcg
     4261 atgaagtatt aacgctaaag tttgattgcc gcccatgcga gatgcacgtc attggcaaaa
     4321 tcaaaaatca catactgaaa atgccgttac cgggaagcgt agtggcatcc gtaagtccgg
     4381 atgagctgct gaaaacattg ccgaagcgaa aagggtaaaa cgccagtttt ctggttactc
     4441 acaacttatt gaatctgcat gatattgcct gccgggtaag gcgttacgcc gcatccggca
     4501 tcaaacgact caacgttgtc ccgcttctgg tttgcgcgat ttttgccagt aaaaatgctc
     4561 gcgcaaacct tccgccgact cttccgccac agcacgcaat tcatcgctgt ccgcttcatg
     4621 acgcagctga tgatccacat tctttaccca cacaaattca tgtcctttgt gccctgccat
     4681 gagttgtcct gagaacagcg cacacgttaa taagacaacc gataacgcct tcgtaaacat
     4741 cctgccacct ttttcttacc ttttgccgct atgatgccga tcgtttcttg aggttattat
     4801 tcagttttgc aaattagcgc aaagaaattc tggaatcttc cttcctgatt ttgcattgca
     4861 ttctgccgtt gcggcgattt agtgctattt tcgagcaaat tacacacgga ggtaaacact
     4921 aatgtttact ttgttgatat actcagccgc agggaggcga tgtgaaacaa agcgagttca
     4981 gacgttggct cgaatctcag ggcgtcgatg tagcgaatgg cagcaaccat ttgaaactca
     5041 ggtttcatgg gaggcgcagt gtcatgccgc gtcacccctg cgatgagatt aaagaaccat
     5101 tgcgtaaagc aatcctgaaa caactcggtt tgagttaatc gccaattaaa aaggttaatg
     5161 acatgcgaga gacagtcgaa attatgcgtt atcccgtcac tcttacaccc gcgccggaag
     5221 gcggttatat ggtttctttt gtggatatcc ctgaagcgtt gacgcagggc gaaactgtcg
     5281 ctgaagcgat ggaagcggca aaagatgctt tactgaccgc atttgatttt tattttgaag
     5341 ataacgagct tatcccttta ccttcgccat taaatagtca tgatcacttt attgaagtac
     5401 ctttgagcgt cgcctctaag gtattgctgt taaatgcttt tttacagtca gaaatcactc
     5461 agcaagagtt agccaggcga attggcaaac ctaaacaaga gattactcgc ctatttaact
     5521 tgcatcatgc gacaaaaatc gacgccgtcc agctcgcggc aaaggcgctt ggcaaagagt
     5581 tatcgctggt gatggtttaa ttacagttaa cgaaaagttg tcatttttaa caactgatat
     5641 agactgccga atcatctgca cataattacg attcgataat gaaaaaatac cagcagcttg
     5701 cagaacaatt acgcgagcag attgcgtcgg gtatctggca acccggcgat cgtttacctt
     5761 cgttgcgtga ccaggtggcg ctttctggca tgagctttat gactgtcagc catgcctatc
     5821 agttgctcga aagtcaggga tatattatcg cacgaccgca gtcgggttat tacgttgcgc
     5881 cacaggcaat aaaaatgccg aaagtgccag tcattccagt cactcgagat gaagcagtcg
     5941 atatcaacac ttatattttt gatatgttgc aggccagtcg cgatccgtcg gtcgttccgt
     6001 ttgcctcggc ctttcccgac ccgcgacttt tccccctcca acaactaaac cgctcgctgg
     6061 cgcaggtaag caaaaccgcc acggcgatga gcgtgattga aaacttgccg ccaggaaacg
     6121 cagaactgcg tcaggctatt gcccgtcgct atgccttaca ggggatcacc atttctcctg
     6181 atgaaattgt catcactgcc ggggcgttag aggcattaaa cctcagtttg caagcggtaa
     6241 ctgaaccggg cgattgggtg atagtagaga atccttgttt ctacggtgcg ttgcaggcgc
     6301 tggagcggct acggctgaag gcgttatcgg tggcgacgga tgttaaagaa gggatcgatc
     6361 ttcaggcgct ggaactggcg ttgcaggagt atccggtgaa agcgtgctgg ctgatgacta
     6421 atagccagaa tccactcgga tttaccttaa cgccgcaaaa aaaagcacaa ctggtggcgt
     6481 tgctcaatca gtacaacgta acgctgattg aagatgacgt ttacagcgaa ctttattttg
     6541 gacgggaaaa accgctgcct gcgaaagcgt gggatcgcca cgatggcgtt ttgcattgct
     6601 cttcgttttc gaaatgtctg gtgcctggtt ttcgtattgg ttgggtcgcc gccggaaaac
     6661 atgcacgtaa aattcaacgc ttgcagctga tgagtacgct ttccaccagc tcaccgatgc
     6721 agcttgcgct ggtggattac ctttccacgc gccgatacga cgcccatctt cgtcgcctgc
     6781 gtcgccagct tgcggaacgt aaacaacgtg cctggcaggc actgctgcgt tatctgcctg
     6841 cggaagtgaa aattcatcat aatgacagcg gttattttct ctggctggag ctccccgagc
     6901 cgttagatgc cggcgaattg agcctggcgg cactgacgca tcatatcagt attgcgccag
     6961 gtaaaatgtt ttctaccggt gaaaactggt cacgtttttt tcgttttaat accgcgtggc
     7021 tgtggggaga acgtgaagaa caggcggtaa aacaattagg caaacttatt caagaacggc
     7081 tgtaatagcg tttaatttaa ttcctcttag attgggtaat atgaatttcg aatagcagtc
     7141 atatttccta actccttgtc tatactccag aagataacct tacagacggc ataatgcgcg
     7201 gtagctcaca acctgattaa attttctcag gggcgaaggt gtgcctgcaa gccgccgtct
     7261 atggttaaac aaggagatat ttttacggca cggcggctga acaattaatt acgacaggag
     7321 taagacctta tgagcaagac atttgcccgc agcagcctgt gtgcgctcag catgacaata
     7381 atgaccgctc acgccgccga accgcctacc aatttagata aaccggaagg gcgactggat
     7441 attatcgcct ggccgggata tatcgaacgc ggacaaactg ataaacaata cgactgggta
     7501 acgcaattcg aaaaagagac aggctgcgcg gtgaatgtta aaaccgccgc gacttccgat
     7561 gagatggtca gtctgatgac caaagggggt tacgatctgg ttacggcatc cggcgatgcc
     7621 tcgctgcgtt tgattatggg taaacgcgtg cagccgatta ataccgcctt gattcccaac
     7681 tggaaaacgc ttgatccgcg cgtggttaaa ggcgactggt ttaacgttgg cggcaaagtt
     7741 tacggcacac cttaccaatg ggggccgaac ctgctgatgt acaacactaa aaccttcccg
     7801 acgccgccgg atagctggca agtggttttt gttgagcaaa atctgcagga cggcaagagc
     7861 aataaaggcc gcgttcaggc ttatgatggc cctatctaca ttgcggacgc tgcgttgttc
     7921 gttaaagcca ctcagccgca gttgggcatc agcgatccgt atcaactcac cgaagaacag
     7981 taccaggcgg tgctgaaagt gctgcgtgat caacatagtt tgatccatcg ctactggcat
     8041 gacactaccg tgcaaatgag cgatttcaaa aacgagggtg tggtcgcttc cagtgcctgg
     8101 ccctatcaga ccaacgccct gaaagccgaa ggtcagcctg tcgctaccgt tttcccgaag
     8161 gagggcgtta ccggttgggc tgacaccacc atgctacata gcgaagcgaa acatccggtt
     8221 tgcgcctaca aatggatgaa ctggtcatta acgccaaaag tgcagggcga tgtggcggcc
     8281 tggtttggct cgttaccagt cgtgccggaa gggtgtaaag ccagttcgtt attaggcgag
     8341 aaaggttgtg aaacaaacgg ttttaactat ttcgataaaa tagccttctg gaaaacgcct
     8401 atagcagaag ggggcaagtt tgttccctac agtcgctgga cgcaggatta cattgccatt
     8461 atgggcggtc gctaacttcg ctggggtgct ttatgacgta cgcagtggag tttgacaacg
     8521 tctcgcggtt gtacggtgac gtgcgcgcag tagatggcgt cagtattgcg ataaaagatg
     8581 gtgagttctt ctctatgctg gggccgtccg gctccggcaa aaccacctgc ctgcgcctga
     8641 ttgctggctt cgaacagctt tccggcgggg ctatctctat ctttggtaaa cccgccagca
     8701 atctgccacc gtgggagcgg gacgtgaata ctgtctttca ggactacgcg ctatttccgc
     8761 atatgtcgat tcttgacaat gtcgcctatg ggctgatggt caaaggcgtg aataaaaagc
     8821 agcggcacgc aatggcgcaa gaggcgctgg agaaagtggc gttggggttt gtacagcaac
     8881 gtaaaccgtc acaactttct ggtggtcagc gccagcgggt tgctatcgcc agagcattgg
     8941 tgaatgaacc gcgcgtattg ctgttggatg aaccgctcgg cgcactggat ctcaaattgc
     9001 gtgagcagat gcagcttgag ctgaaaaaac tgcaacagtc tctcggtatc acttttatct
     9061 gcgttaccca cgatcagggc gaagcgttat cgatgtccga tcgtgtggcg gttttcaata
     9121 atggacgcat tgagcaggtc gattccccgc gcgatctcta tatgcgcccg cgcacgccgt
     9181 ttgttgccgg gttcgttggt acatcgaatg tttttgatgg actgatggca gagaaacttt
     9241 gtggcatgac gggaagcttc gccctgcggc cggaacatat ccgcctcaac acccctggtg
     9301 aactgcaggc caatggcacg atccaggcgg tgcaatatca gggcgcggca actcgttttg
     9361 aactgaaatt gaacggcggt gaaaaactgc tggtgagtca ggccaatatg acaggcgaag
     9421 aactgcctgc cacgctcacg cccggacaac aggtgatggt ttcctggtcg cgtgatgtga
     9481 tggtgccgct ggttgaggag aggtgaatgg cgatgaacgt attgcaatca ccttcacgtc
     9541 caggtctggg taaggtgtcc ggtttcttct ggcggaatcc ggggctgggg ctgtttttgc
     9601 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctaacgctgt
     9661 tatggcaggg cttttatacc tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     9721 tggcgaacat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     9781 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     9841 tggcgcgcta taccagcggg aaaatgaaag cgttttttta tattgcggta atgttgccga
     9901 tgtgggcgag ctacattgtt aaagcctatg cctggacgtt attgctggca aaagatggcg
     9961 tggctcagtg gtttttacaa catcttgggc tggaaccact gctgacagcg ttccttacat
    10021 tacctgcggt gggcggcaat acgctgtcaa cttccgggct ggggcgcttt ctggtgtttc
    10081 tctatatctg gctgccgttc atgatcctgc ccgtgcaggc ggcgcttgag cgtttgccgc
    10141 cgtcattgtt gcaggcgtca gctgatctcg gcgcacgtcc acgacaaacc tttcgctatg
    10201 tggtgttgcc gctggcgatc ccgggtattg ccgctggctc tatctttacc ttctcactca
    10261 cactgggcga ttttatcgtc ccgcagctgg ttggcccgcc aggatatttt atcggcaata
    10321 tggtttattc ccagcagggg gcgattggca atatgccgat ggcggcggca ttcaccctgg
    10381 tgccgattgt tcttatcgca ctgtacctgg cgttcgtgaa acgtctggga gcgtttgatg
    10441 cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt tgttttccta
    10501 cattttccca tcctgataat cgccgcctat gcgtttaaca ctgaagatgc ggcgtttagt
    10561 tttccaccgc agggcctgac gctgcgctgg tttagcgtgg cagcacagcg tagtgatatt
    10621 cttgatgccg tgacactgtc acttaaagtg gcggcgctgg cgacattaat ggcgctggtg
    10681 ttagggacgc tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa aaacgccatt
    10741 tcgctgttac tgctgctgcc cattgcgctg ccgggcattg tcactggtct ggcgttatta
    10801 accgccttta aaaccatcaa tctggagtcg ggatttttca ccatcgtggt cggtcatgcg
    10861 actttttgtg tagttgtggt gtttaacaat gtcatcgccc gttttcgccg cacctcatgg
    10921 agtctggttg aggcgtcaat ggatcttggg gccaatggct ggcaaacctt ccgctacgta
    10981 gtgttgccga atctcagttc ggcgttactg gcaggaggaa tgctggcgtt tgccttgtcg
    11041 ttcgatgaaa tcatcgttac gacctttacg gcaggtcatg aacgaacgtt accgttgtgg
    11101 ttgctcaatc agcttgggcg accgcgtgat gtaccggtaa ctaacgtggt ggcactgctg
    11161 gttatgttgg taacaacctt gccgatcctg ggagcctggt ggctaacccg cgaaggcgac
    11221 aatggtcaat aaccactgat acaggaatat gctatgcaac ataagttact gattaacgga
    11281 gaactggtta gcggcgaagg ggaaaaacag cctgtctata atccggcaac gggggacgtt
    11341 ttactggaga ttgccgaggc atccgcagag caggtcgatg ctgctgtgcg cgcggcagat
    11401 gcagcatttg ccgaatgggg gcaaaccacg ccgaaagtgc gtgcggaatg tctgctgaaa
    11461 ctggctgatg ttatcgaaga aaatggtcag gtttttgccg aactggagtc ccgtaattgt
    11521 ggcaaaccgc tgcatagtgc gttcaatgat gaaatcccgg cgattgtcga tgtttttcgc
    11581 tttttcgcgg gtgcggcgcg ctgtctgaat ggtctggcgg caggtgaata tcttgaaggt
    11641 catacttcga tgatccgtcg cgatccgttg ggggtcgtgg cttctatcgc accgtggaat
    11701 tatccgctga tgatggcagc gtggaaactg gctccggcgc tggcggcagg gaactgcgta
    11761 gtgcttaaac catcagaaat taccccgctg accgcgttga agttggcaga gctggcgaaa
    11821 gatatcttcc cggcaggcgt gattaacgta ctgtttggca gaggcaaaac ggtgggcgat
    11881 ccgctgaccg gtcatcccaa agtgcggatg gtgtcgctga cgggctctat cgccaccggc
    11941 gagcacatca tcagccatac cgcgccctcc attaagcgta ctcatatgga acttggtggc
    12001 aaagcgccag tgattgtttt tgatgatgcg gatattgaag cagtggtcga aggtgtacgt
    12061 acatttggct attacaatgc cggacaggat tgtactgtgg cttgtcggat ctacgcgcaa
    12121 aaaggcattt acgatacgct ggtggaaaaa ctcggtgctg cagtggcaac gttaaaatct
    12181 ggtgcgccgg atgacgagtc tacggagctt ggacctttaa gctcgctggc gcatctcgaa
    12241 cgcgtcagca aggcagtaga agaggcgaaa gcgacagggc acatcaaagt gatcactggc
    12301 ggtgaaaagc gcaagggtaa tggctattac tatgcgccga cgctgctggc tggcgcatta
    12361 caggacgatg ccatcgtgca aaaagaggta tttggtccag tagtgagtgt tacgctcttc
    12421 gacaacgaag aacaggtggt aaactgggcg aatgacagcc agtacggact tgcatcttcg
    12481 gtatggacga aagatgtggg cagggcgcat cgcgtcagtg cacggctgca atatggttgt
    12541 acctgggtca atacccattt catgctggta agtgaaatgc cgcacggtgg gcagaaactt
    12601 tctggttacg gcaaggatat gtcactttat gggctggagg attacaccgt cgtccgccac
    12661 gtcatggtta aacattaagg ataatattgc agatcgtaag agtatgagat gatcttgcgt
    12721 actgtcagag cactgatttc tctgacagta cgtgctgcgt gcaaaatcac tacatcaaat
    12781 aaaccagcca aatctcatag agataagagt aaacaaacca acagcagcaa agacatttat
    12841 cagtactaca gttctactgg aaacattcat ttttgccacc ttttatcatt gcccctatgt
    12901 gtatagtacg ggttaagaaa atccgcaata atactcattc tgaagatgga taagggcaag
    12961 ttgctgtttg atgttttttt ctcaaaccgg ttaaaaaact tgatgcacgt caaaaaatga
    13021 cgcatatttg cgcgcgtttt attcatctgg ctggacgccc gtacatgtct ctctatcaac
    13081 acatgcttgt tttttatgcg gttatggcag caatcgcatt tcttatcact tggtttcttt
    13141 ctcacgataa gaaacgcatc cgtttcttaa gcgcctttct ggtgggggca acatggccga
    13201 tgagtttccc ggtggcgctg ttgttttcac tgttttaaat cttactcact ggcttgacgg
    13261 caccacgcag acttatatca tttggatgaa tcgataaatt tcacaagtgg ctaaggagaa
    13321 agtatgtcgc atctggatga agtcatcgcg cgcgtggatg ccgcgattga agagagcgtc
    13381 attgcccata tgaacgaatt attaatcgcc ctgagcgatg acgcggagtt aagtcgggaa
    13441 gatcgctaca cccagcagca gcgtttgcgc accgctattg ctcatcacgg tcgcaagcat
    13501 aaagaagata tggaagcgcg ccatgaacag ttaaccaaag gcggcaccat cctgtgatta
    13561 aaatgagcgt ctggctacca accaggcgcc aaccacaagt aaaattgcgc cacaaattgg
    13621 gccgaacaac gcccgtagtg gtacgccatg actgcggaag atatccagaa ccagaccgcc
    13681 aatcagctgg ctggcgacca gtactgcaat agttgtcgcc gctcccacat tctgatatcc
    13741 gctgatactg gcgaagacaa aaaacgatcc cagcaagcca ggtataagtg tccaccagcg
    13801 cacgctggac accagttcgc caaaccccgc cataccttgt ttaaaccata aaatagaaac
    13861 aaagagcaca atccccacca gtgagttcag caacatggcg atgagaatgg tagaggaggt
    13921 ctgggtgatg cgcaccatca gcgagttctg gactaccaga ccaattccgg cggcaatcaa
    13981 aaaggcaagg gtgagcgact gattcatcca atcgcgtccg gttcagtgcg ttcgtcgagt
    14041 tgcaactgca taaatgtcag atccagccag cgcccaaatt tagtgcccac ctgcggcatt
    14101 tgtgcggtga cgacaaatcc cagcgactgg tgcagatgca gcgaggcctg attttgcgat
    14161 tcgatcccgg cgaccatgac atgcttcccg caatctcgcg cttcatcaat caatcggctt
    14221 aacaatttac gccccagacc tttgccctga tgatcgggat ggacataaac cgaatgttcc
    14281 acggtatggc gaaaaccatc gaaactacgc cagtcgccaa atgaggcata tcccgtcact
    14341 acgccatctt cttcacttac cagcaccgga taacctgcta tggtccgcgc ttcaaaccag
    14401 gcaatgcggt tatctgcatc caccgtttgg tcattccaga tagccgccgt atacaacacg
    14461 gcgtggttat aaatttccgc aatggcagca cagtcggctt tgcgggcaaa acggatggac
    14521 atgtctggcc tcaataaaat aatgatgatg atgttatcaa tactatcgaa aacagcccga
    14581 agcaacggat tcctcgatga aattattgcg tgttgcagag ggggggtgac ggctgcaaac
    14641 aaaaccaatc ctgcgagcct gctactattg atggattcat gccaaaagca caggagagca
    14701 ttatggggca acaaaagcag cgtaatcgac gttgggttct ggcctcgcgt ccacatggcg
    14761 caccggttcc ggagaatttc cgtcttgaag aagatgatgt cgccacaccg ggtgaaggac
    14821 aggtgttact gcgcacagtt tatttgtccc tggacccgta tatgcgtgga cgtatgagcg
    14881 atgagccatc ttattcaccg ccggttgata ttggcggcgt gatggtcggc ggtacggtga
    14941 gccgtgtcgt ggagtcgaat catcctgatt atcagtctgg cgactgggtg ctgggctaca
    15001 gtggatggca agactatgac atatccagtg gtgatgatct ggtgaaactt ggcgatcatc
    15061 cgcaaaatcc atcgtggtcg ctgggtgtgc tagggatgcc aggctttacc gcttatatgg
    15121 gcctactgga tatcggtcag cctaaagagg gcgaaacgtt ggtggtagct gcggcgacag
    15181 gaccagtggg ggcgacggtg gggcaaatcg gcaaacttaa aggttgcaga gtggtggggg
    15241 tagccggtgg cgcggaaaaa tgccgccatg ctaccgaggt gttaggcttc gatgtttgtc
    15301 ttgatcacca cgcggatgat tttgccgaac aactggcgaa agcgtgccca aaaggtattg
    15361 atatctatta tgaaaacgtg ggcggtaagg tattcgatgc ggtgctaccg ttacttaata
    15421 catctgcgcg cattcccgtc tgcggattag tgagcagcta taacgctaca gagctaccac
    15481 ccggtccgga tcgtttacct ctgttgatgg ctacagtgct gaaaaaacgt attcgcttgc
    15541 aaggttttat tatcgctcag gattatggtc accgcatcca tgagtttcag agggagatgg
    15601 ggcaatgggt gaaagaggat aaaatccact accgcgaaga aattactgac ggtttagaga
    15661 atgcgccaca gacgtttatc ggcctgctga agggtaaaaa cttcggcaaa gtggtgatcc
    15721 gcgtggcggg tgatgattaa agtttcaacg gcggcgtaag ccgccataaa aatccaggca
    15781 aaaagcttat tttcagcttt aattaactaa ccacatcatt gttctgctgc taataacaac
    15841 aaaggtgagt ggtttatgat tattcacttt aatacaccag gtgaattcct tctgccatgc
    15901 aggcagggtt ggacagaaaa cacgcgaagg aattaaaatg cctggaacgg aaaaaatgaa
    15961 acatgtcagt ttgactctgc aggttgagaa cgacctgaaa catcagctta gtattggcgc
    16021 actaaaacct ggcgcacgcc tgattactaa aaatctggcg gagcaattag gtatgagtat
    16081 tacacctgtg cgtgaagcat tattacgtct ggtttcggtg aatgcgcttt ctgtcgcacc
    16141 agcgcaagca tttacagttc cggaagtggg gaaacgtcaa ttagacgaaa tcaatcggat
    16201 ccgctacgaa ctggaattaa tggcagttgc tctggctgtt gaaaacctca ccccgcaaga
    16261 cctggcggaa ctccaggaat tgctcgagaa attacagcag gcgcaagaaa agggcgacat
    16321 ggaacaaatc attaatgtaa acaggctatt tcgcttagcg atttatcatc gctcaaatat
    16381 gcccatcctg tgtgagatga ttgagcaact gtgggtcagg atgggacctg gtttacatta
    16441 tctctatgaa gcgattaatc cagcggaatt acgggagcat atagaaaact atcatctgtt
    16501 actcgctgcg ttaaaagcaa aagacaaaga gggatgcaga cattgtcttg ctgaaattat
    16561 gcaacaaaat attgctattt tataccagca atacaatcgt taaagtaaat gcccggcaaa
    16621 taccgggcat caaggtaatt actcaaatcg ccatgcgata ttcacgccga cacca
//
LOCUS       DBFIFS010000089        16604 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.089, whole genome shotgun sequence.
ACCESSION   DBFIFS010000089 DBFIFS010000000
VERSION     DBFIFS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.089"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            96..677
                     /gene="ddpX"
                     /locus_tag="N7A07_002580"
     CDS             96..677
                     /gene="ddpX"
                     /locus_tag="N7A07_002580"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU2477218.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            691..2241
                     /locus_tag="N7A07_002581"
     CDS             691..2241
                     /locus_tag="N7A07_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2477219.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            2243..3265
                     /locus_tag="N7A07_002582"
     CDS             2243..3265
                     /locus_tag="N7A07_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477220.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            3262..4158
                     /gene="ddpC"
                     /locus_tag="N7A07_002583"
     CDS             3262..4158
                     /gene="ddpC"
                     /locus_tag="N7A07_002583"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU2477221.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQV
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            4155..5141
                     /locus_tag="N7A07_002584"
     CDS             4155..5141
                     /locus_tag="N7A07_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2477222.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            5134..6060
                     /locus_tag="N7A07_002585"
     CDS             5134..6060
                     /locus_tag="N7A07_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU2477223.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(6116..6547)
                     /gene="osmC"
                     /locus_tag="N7A07_002586"
     CDS             complement(6116..6547)
                     /gene="osmC"
                     /locus_tag="N7A07_002586"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU2477224.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            6892..7107
                     /gene="bdm"
                     /locus_tag="N7A07_002587"
     CDS             6892..7107
                     /gene="bdm"
                     /locus_tag="N7A07_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU2477225.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7209..7346
                     /gene="sra"
                     /locus_tag="N7A07_002588"
     CDS             7209..7346
                     /gene="sra"
                     /locus_tag="N7A07_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU2477226.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            7503..9200
                     /gene="maeA"
                     /locus_tag="N7A07_002589"
     CDS             7503..9200
                     /gene="maeA"
                     /locus_tag="N7A07_002589"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU2477227.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            9334..10344
                     /gene="adhP"
                     /locus_tag="N7A07_002590"
     CDS             9334..10344
                     /gene="adhP"
                     /locus_tag="N7A07_002590"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU2477228.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            10490..10774
                     /locus_tag="N7A07_002591"
     CDS             10490..10774
                     /locus_tag="N7A07_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU2477229.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(11002..11655)
                     /gene="fdnI"
                     /locus_tag="N7A07_002592"
     CDS             complement(11002..11655)
                     /gene="fdnI"
                     /locus_tag="N7A07_002592"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU2477230.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(11648..12532)
                     /gene="fdnH"
                     /locus_tag="N7A07_002593"
     CDS             complement(11648..12532)
                     /gene="fdnH"
                     /locus_tag="N7A07_002593"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU2477231.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(12545..15592)
                     /gene="fdnG"
                     /locus_tag="N7A07_002594"
     CDS             complement(12545..15592)
                     /gene="fdnG"
                     /locus_tag="N7A07_002594"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15005..15007),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU2477232.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            15824..>16604
                     /gene="yddG"
                     /locus_tag="N7A07_002595"
     CDS             15824..>16604
                     /gene="yddG"
                     /locus_tag="N7A07_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU2477233.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALA"
BASE COUNT         3961 a         4335 c         4283 g         4025 t
ORIGIN      
        1 aactttaagc cagcattctg gcgcttatcc cggcatggca tgagatctgc ataagcggaa
       61 agcgcagcaa tttttgtctt atacaggaac cgtttatgtc ggataccacc gaactggttg
      121 atttagccgt gatcttccct gatctggaga tcgaattgaa atacgcctgc gctgataaca
      181 tcacaggtaa agctatttac cagcaagcgc gttgtctgtt acacaaggat gcgattaccg
      241 cgctggcgaa aagtatcagt atcgcccagc tatcaggctt acaactggtg atttacgatg
      301 cgtatcgccc acaacaagca caggcgatgt tgtggcaagc ctgcccagac ccgcaatatg
      361 ttgttgatgt gacggtcggt tctaatcaca gccgtggcac ggcgatcgac ctgacgcttc
      421 gtgatgagca cgggaacatc ctggatatgg gcgcagggtt cgatgaaatg cacgagcgct
      481 cccatgccta tcacccttcc gtcccgcccg ccgctcagcg caatcgcctg ttgctgaatg
      541 cgataatgac tggtggcggt tttgtcggta tctccagcga atggtggcac ttcgaattac
      601 cccaggcagc gagttaccct ctgctcgctg atcaattcac ctgttttata tcccctggca
      661 cacagcacgt cagttaatca ggaaactgcc atgaagagat cgatatcgtt tcgtcccaca
      721 ttgctcgcgc tcgtccttgc cacaactttc ccggttgcgc acgccgccgt accgaaagat
      781 atgctggtga ttggtaaggc cgccgatcca caaaccctcg acccggcggt aacaatagat
      841 aataacgact ggacagtgac ctacccgtct tatcagcgac tggttcagta caaaacggac
      901 ggtgataaag gctcaaccga cgttgaaggc gatctggcaa gtagctggaa agcgtctaac
      961 gatcaaaaag agtggacgtt caccctgaaa gataatgcta aatttgccga tggcacacct
     1021 gtcactgccg aagcagtaaa actctctttt gagcggttac taaaaatcgg ccaggggcca
     1081 gcagaagcat ttcccaaaga tttaaagatt gatgctcccg acgaacatac ggtgaagttt
     1141 acccttagcc agccatttgc accgttcctc tacacgctgg cgaatgacgg tgcatccatt
     1201 atcaatccgg cggtattaaa ggaacatgcg gcggatgatg cccgcggctt cctcgcgcaa
     1261 aataccgccg gttccggacc atttatgctg aaaagctggc aaaaaggtca gcaattagtt
     1321 ctggtgccaa atccgcatta ccccggcaat aaaccgaact ttaagcgagt atcggtaaaa
     1381 attatcggtg aaagtgcctc ccgtcgcctg cagctctccc gtggcgacat tgacattgcc
     1441 gatgcgctgc cggtggatca actcaacgcc ctgaagcagg aaaacaaagt caatgtggca
     1501 gagtatccgt cactgcgcgt cacctatctg tatctgaata acagcaaagc gccacttaat
     1561 caggcggatc tgcggcgggc catttcctgg tctaccgatt atcagggcat ggttaacggc
     1621 attctgagtg gtaacggaaa acaaatgcgc ggcccgattc cggaaggcat gtggggctac
     1681 gatgcgacgg caatgcaata caaccatgac gaaacgaaag ccaaagccga atgggataaa
     1741 gtgacgagca aacccaccag cctgacgttt ctctactccg ataacgatcc gaactgggaa
     1801 ccgatcgctc tggcaacaca atccagcctc aacaagctgg gcatcaatgt gaagctggaa
     1861 aagctggcga acgccaccat gcgcgacaga gtgggtaaag gtgattacga cattgcgatt
     1921 ggcaactgga gtccggattt tgccgacccg tatatgttta tgaattactg gtttgagtca
     1981 gacaaaaaag gtctgccggg taaccgctcg ttctatgaaa acagtgaggt cgataagtta
     2041 ctgcgcaatg cgcttgcgac caccgaccag acgcagcgta cccgagacta ccagcaggca
     2101 cagaaaatcg tcattgatga cgctgcttat gtgtacctgt tccagaaaaa ctaccaactg
     2161 gcgatgaaca aagaggtgaa aggctttgtg ttcaatccca tgctggaaca ggtcttcaat
     2221 atcaatacca tgagtaaata acgtgacctt ctggagtatt ttacgccaac gctgctgggg
     2281 gctggtgctc gtggtggcgg gcgtctgcgt gattacgttt attatctcgc atctgatccc
     2341 cggcgatccg gcgcggttac tggcgggtga ccgcgccagc gatgctatcg tggaaaatat
     2401 tcgccagcaa ctgggactgg accagccact gtatgtacag ttttaccgct acgtcagcga
     2461 cctgtttcat ggtgacctgg gaacatccat tcgtactggg cgtccggtgc tggaagagtt
     2521 gcgtatattt ttcccggcga cgctggagct ggctttttgt gccctgctgc tggcactctt
     2581 gattggcatc ccgctgggca tactctctgc agtctggcga aatcgctggc tggatcatct
     2641 ggtgcgaata atggcgatta ccggaatctc cacacctgcg ttctggcttg ggctgggcgt
     2701 catcgtgctg ttttatggtc atctgcaaat acttcccggc ggcggaaggc ttgatgactg
     2761 gctggatcca ccaacgcacg ttaccggctt ttatctgctc gatgcgttgc ttgaaggcaa
     2821 cggtgaagtc ttcttcaatg cgttgcaaca tctcatctta ccggcattaa cgctggcgtt
     2881 tgttcacctg gggattgtcg cccgccagat ccgctcagcg atgctggaac aattgagtga
     2941 agactacatt cgtaccgccc gggccagcgg cttgcccggc tggtatatcg ttctatgtta
     3001 tgcgctaccc aatgcgttga tcccttcgat taccgtattg gggctggcgc tgggcgattt
     3061 gttgtatggc gcagtgctca ccgaaaccgt ttttgcctgg cccggaatgg gggcatgggt
     3121 agtgacatca atacaggcgc tcgacttccc ggcagtgatg ggctttgccg tcgtggtttc
     3181 atttgcttat gtgctggtca acctggtggt ggatttgctc tatttgtgga ttgatccgcg
     3241 tatcggacgt ggaggtggtg aatgatgcta agcgaggaaa cgtccgccgt acgcccacaa
     3301 aaacaaacgc gatttaacgg tgcaaaactg gtatggatgc tgaaaggcag tccgctcacc
     3361 gtgaccggcg cagtcatcat tgtattaatg ctactgatga tgattttttc accgtggctg
     3421 gcgacgcatg atcccaacgc cattgattta accgcccgcc ttttgccgcc ttctgcggcg
     3481 cactggtttg gcaccgatga agtgggacgc gatctgttca gccgcgtact ggtcggcagt
     3541 cagcaatcaa ttctcgccgg attagtcgtg gttgccattg cgggtatgat tggttcgcta
     3601 ctcggatgtc tatccggtgt gcttggcgga cgcgcagacg ccatcatcat gcgcatcatg
     3661 gacattatgc tgtcgattcc ttcgttggta ctgacaatgg cactggctgc cgctctcggg
     3721 ccgagtttgt ttaacgccat gctggctatt gctattgtgc gaattccctt ttatgtgcgc
     3781 ctggcgcggg ggcaagcatt agttgtacgc cagtatacct atgttcaggc ggcgaaaacc
     3841 tttggcgcgt ctcgttggca tctgatcaac tggcatattt tacgtaactc cctaccgccg
     3901 ctgatcgtgc aggtatcgct ggatatcggt agcgcgattt taatggccgc cacgttggga
     3961 tttattggcc tgggtgctca acaaccgagt gctgaatggg gcgcgatggt agcaaatggt
     4021 cgcaactatg tactcgatca gtggtggtat tgcgcatttc cgggggcagc gattttgctt
     4081 accgccgtcg ggtttaatct ctttggtgat ggtattcgcg atctgcttga cccgaaagca
     4141 ggaggaaagc agtcatgacc caacccgttc tggacattca acaactgcat ttgagtttcc
     4201 ccggttttaa cggcgacgtt cacgcgctca acaatgtgtc cttgcagatt aaccgcggtg
     4261 aaattgtcgg tctggtggga gaatccggct caggtaaatc agtcaccgca atgctgatta
     4321 tgcgtctgct accgacgggc agttattgcg tacatcgggg gcagatttca ctgctgggag
     4381 acgatgttct taacgcgcgg gaaaaacagc ttcgtcagtg gcgcggcgca cgagtggcga
     4441 tgatctttca ggaaccgatg accgccctca atccgacacg tcgaataggt cttcagatga
     4501 tggacgtgat ccgccatcat caaccaataa gtcgtcggga agccagagct aaagcgattg
     4561 ccctgctgga agagatgcaa atcccggata ccgtggaagt tatgtcgcgc tatccgtttg
     4621 agctttcagg tggtatgcgc cagcgggtaa tgattgcgct ggcattctcc tgcgaaccgc
     4681 aattgattat tgccgacgaa ccgactacgg cgctggacgt cacggtacag ttgcaggtac
     4741 tgcgtttgct taaacataaa gctcgcgaca gtggaactgc ggtactgttc atcagccatg
     4801 atatggccgt ggtgtcgcaa ctgtgcgata gcgtttacgt gatgtatgcc ggaagcgtga
     4861 ttgagagcgg cgtgacggca gacgttatcc atcatccccg gcatccgtat accattggtt
     4921 tgctgcaatg cgcaccagaa catggagtac cacgccagcc attacccgcc attccaggga
     4981 cggtaccaaa tctcacccat ttgcctgacg gctgcgcttt tcgcgatcgt tgctatgcgg
     5041 caggtgcaca gtgtgaaaac gtcccggcgc tgacagcgtg tggtgacaac aaccagcgct
     5101 gcgcctgttg gtatccgcag caggaggtca taagtgtctg acacgttatt aacgttacgc
     5161 gacgtccata tcaatttccc ggcgcgtaaa aactggcttg gtaaaactac ggaacatgtt
     5221 catgccatta atggtattga tttacagatc cgccgtggtg aaaccttagg gatcgtcggc
     5281 gagtcaggct gcggcaaaag caccctcgca cagcttttaa tgggtatgct gcaaccgagc
     5341 cacgggcagt acatccgttc tggctcacaa cgcattatgc agatgatgtt tcaggacccg
     5401 ctctcttcgc ttaatccgcg cttaccggtg tggcgcatca tcacagaacc gctctggata
     5461 gctaagcata gtagtgaaca acagcggcga gcgttggcag aggagctggc tgtgcaggtg
     5521 ggtattcgtc cggagtatct cgaccgcctg cctcatgcgt tctccggcgg gcagcggcaa
     5581 cgcatcgcca ttgccagagc actctcttcg cagcctgacg tgattgtgct tgatgagcca
     5641 acctctgcgc tggatatctc cgtgcaggcg cagatcctca atttactggt aacgctacag
     5701 gaaaatcacg ggctgactta tgtgctgatt tcacacaatg tctcggtgat acgtcatatg
     5761 agcgatcggg tggcggtgat gtatctcggg cagattgtgg aattagggga agcgcaacag
     5821 gtgctaacgg ctccggcaca tccatacacc cgattattgc tggattccct ccccgccatt
     5881 gataaaccac tggaggaaga atgggcatta cgtaaaacgg atctgccagg aaaccgcacg
     5941 ttgccacagg gctgtttttt ccgcgaacgt tgcccccttg caaccagcgg atgcgaagtc
     6001 cggcaatcat taacgacgcg ggctgacgga cgtgagatcc gttgctggcg ggcgctgtag
     6061 agatgctaac aacgcatcag gcattcatgc gcctgacgcg tcatccggca atgctttacg
     6121 atttcaactg gtaatccagc gtaatttccg ctttcagtac ctgagagacc gggcatcctg
     6181 cttttgcttt ctggatgatg ccgtcaaagg tagaggcatc aatacccggc accgcaactt
     6241 cactcttcag tgcgattttc gtaatcgcaa aaccggcatc cactttatcc agcgacacat
     6301 cggcggtggt atcaatcgat gttggcgtga atcccgcttc ccccagcatt aatgaaagcg
     6361 ccattgagaa acatgcggca tgcgctgcgc caatcagttc ttcagggttg gttccttttt
     6421 cgccttcaaa acgcgtgtta aatccatacg gctgttggtt cagcacgcca ctctcggtgg
     6481 atactgttcc cttcccgcgt ttgatatcgc tttcccagtg tgcctgacct ttcttatgga
     6541 ttgtcattgt tgctctcctg tgggcttaaa aaggaaatta tagactacgt gagaaaccgc
     6601 acgaggataa gcaggatatt ccgaataaaa tacggcaaaa atattacttc tgcacttaaa
     6661 ttaaattccc acaatagata accggaaata tatatctcaa gagagattgc atcaatatta
     6721 atcaactcat cacaataata atacctatca atatcatggg gttaaaatat aaaaatcatc
     6781 aaccaggact aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata
     6841 aatctgcgat ccgtagcaga cactataaat acacagacac ggagaatcac tatgtttact
     6901 tattatcagg cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc
     6961 cttcgggcag agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg
     7021 gtggaagctt ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg
     7081 gtcagtggcc agcaccctac gctttaagat gctatgcttg atcggcaacc taatttaggg
     7141 gtttagcacg tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag
     7201 gagttaaaat gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaatat
     7261 ctaaccagcg caaaatagtt accgaaggtg acaaatccag cgtggtaaat aacccaaccg
     7321 gcagaaaacg ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca
     7381 ccattgcctg cgcaatggtg tttttgtttt tatctgcttt atacttgggg ccgacgccct
     7441 ggcggtaaag caaagacgat aaaagccccc cagggatgga tattcaaaaa agagtgagtg
     7501 acatggaacc aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac
     7561 tgctggaatt tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact
     7621 tcaacctgct ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag
     7681 catggatcca gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca
     7741 tccaggacac caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga
     7801 tgcctgttat ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc
     7861 gccgttcacg cggcgtgttt atctcttacc agaaccgcca caatatggac gatattctgc
     7921 aaaacgtgcc gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg
     7981 ggcttggtga ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata
     8041 ccgcctgtgg cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga
     8101 acaaccaaca gctgcttaac gatccgctgt atatgggctg gcgtaatccg cgtatcactg
     8161 acgacgaata ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccag
     8221 acgtgctgtt gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct
     8281 atcgcaatga aatttgttcc tttaacgatg acattcaggg caccgcggcg gtaacagtcg
     8341 gcaccctgat cgcagcaagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct
     8401 tccttggcgc aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc
     8461 gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt tatggtcgat cgctttggcc
     8521 tgctgaccga caagatgccg aacctgctgc ctttccagac caaactggtg cagaagcgcg
     8581 aaaacctcag tgacagggat accgacagcg atgtgctgtc actgctggat gtggtgcgca
     8641 atgtaaaacc agatattctg atcggcgtct caggacagac cgggctgttt acggaagaga
     8701 tcatccgtga gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tccaacccga
     8761 cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg gactgaaggt aacgcgctgg
     8821 tcgccacggg cagtccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc
     8881 agtgtaacaa cgcctttatt ttcccgggca tcgggctggg tgttattgct tccggcgcgt
     8941 cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac gcttgcccag tattcgccgc
     9001 tggtcctgaa cggcgaaggt ctggtactac cggaactaaa agatattcag aaagtctccc
     9061 gcgcaattgc gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt
     9121 ctgccgaagc tttgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact
     9181 accgccgtac ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc
     9241 tccctttttc agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct
     9301 gctatgcaga acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat
     9361 catcatgttg acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg
     9421 aaaatggagt gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt
     9481 gacaaaaccg gcgtaattct gggccatgaa gggattggtg tggtggcaga agtgggtcca
     9541 ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc
     9601 ggtcattgcg aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc
     9661 ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta
     9721 aaagtgccag atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc
     9781 acctacaaag ccgttaagct gtcaaaaatt cgtcccgggc agtggattgc tatctacggt
     9841 cttggcggtc tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgcgaaagtg
     9901 atcgccattg atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta
     9961 gcgattaact cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct
    10021 cacgctgcgg tggtaacagc agtagctaaa gctgcgttta actcggcagt tgatgctgtc
    10081 cgtgcaggcg gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc
    10141 ccacgtcttg tgctggatgg cattgaggtg gtcggttcgc tggtcggcac gcgccaggat
    10201 ctaactgaag ccttccagtt tgccgccgaa ggtaaagtgg tgccgaaagt cgccctgcgt
    10261 ccgttagcgg acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt
    10321 atggtgattg atttccgccg ctaagaggcc tttgctgcga ctcccatgtt cgggtcgcag
    10381 catcgcacac tctccaacat gaaatggcta aatggattat tgttgcgtag gaaggaacaa
    10441 taatgatagt gttcccagag aaggaacgct actgagagta aagacactaa tgaaaatggc
    10501 caatcatccc cgcccggggg acattattca ggaatcactg gacgaactta atgtcagcct
    10561 gcgcgagttt gccagagcaa tggaaattgc tccctcaacg gcaagccgat tgctgaccgg
    10621 aaaagcagct ttgacgccag aaatggcaat taaactttcc gtggtgatcg gcagttcgcc
    10681 gcaaatgtgg ctgaatctgc aaaacgcctg gagtctggca gaggctgcaa aaacggtgga
    10741 tgtgtcgcga ttacgccgtc tggttgcgca ataaaaaatg gcgcctttta cagcgccatt
    10801 tgaaactgat gacaaacgca acattgcctg atgcgctacg cttatcaggc ctacacgttc
    10861 actgcaatct attggatttg aacggtttgt aggccggata aggcgttcac gccgcatccg
    10921 gcatgaacaa agcctacgtt gtctacaata tgaaaatggc gccttttaca gcgccatttt
    10981 cagcttaaag ttcaaaagag attatatccc ttcttcactc tcttttttcg cttctgcctt
    11041 ctcgatttca cgataccagc gcggatggtg tttcttcgcc cagcgacggc tcaccttccc
    11101 ttcgatcatc cctttaatcg atcctttcac ccagaatgcc atatacatat ggatcaggat
    11161 ggcgtggatc aggatgatac ccgcagccgc gtggatcagc aggctgtagc gaacaacctg
    11221 catcgggaag tactgcgcaa agtacggacg ccagataatc accccggtca ccagcagcac
    11281 gaaaatcatg ctcatgatcg accagaacat catcttttgc ccggcgttgt acttaccgac
    11341 atccgccact ttatgctcat tgcctttcaa tacttcgaca atgtttaaca gccacggaat
    11401 atctttctta tccgggatgt tgtgatgcac aaaacgcaca aacataaaca tcagtgcgac
    11461 gaaaatcgca atgccgaaga atgggtgcaa aatgcgtccc atctgcggcg taccgaaggt
    11521 ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa
    11581 gaagcaaatc accacggtcc agtgacaggc gcgatcaata aatttggtgc gcacaatcat
    11641 tttcgactta ctcatgatga tcctcctcgt catcgtccac ttccttattc gggccaatac
    11701 cgatgtagtg gaaaatcaac cctgcaaaag tggcaataaa gccagccgct gccagcggtt
    11761 tcaacgcgcc tttccacagg cttaccgagg tgtcgatctt cggatctttc ggcagaccgt
    11821 gatacagctc cggctgatcg gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc
    11881 cttccgggtt gtagacgcca gcatgttcgt aaccacgcgc tttcagtttc gccacgcgct
    11941 gttccgccag ctccagcatc tccttcttgg tgccgaagtg gatagccccg gtcggacagg
    12001 ttttcacaca agccggttcc tggccgacgc tgacgcgatc gacgcagagc gtgcatttat
    12061 atacccggtt atcctctttg ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt
    12121 aaccacagcc gatgcagttt tccgactgga aatcgacaat cccgttagcg tactgaatga
    12181 ttgcaccggc agacgggcac gccttcaggc agcccggatc ttcacagtgc atacagccat
    12241 ctttacggat cagccactcc agcttgccgc tctgttcggt ttcgctaaag cgcatcaccg
    12301 tccaggactt ggcgctcaga tcggcggggt tatcgtaaac cccgacgcag tgccccactt
    12361 catcacggat gtcgttccac tccgaacacg ccacctgaca ggctttacag ccgatacagg
    12421 tggaaacgtc gataagtttt gcgacttctg ctttgtaatc acgcacctga gaaggcggcg
    12481 tgatggagtt agttgcggac cttttgataa tgtcctgcgt ttccatagcc atctgttcgc
    12541 ccccttacgc cttctcgatg ttgactaaga acgctttata ttccggcgtt tgcgagtttg
    12601 catcaccgac attcggcgtc agagtgttag ctatataacc tttacgcgcg acaccctcaa
    12661 agccccagtg gattggaata cccaccgttt caacctgctg accatttaca ttcagcggtt
    12721 tcagacgacg cgttaccaca gccaccgcgc ggataaagcc acgcttgctt gagacagtga
    12781 cacgatcgcc attattaatg cctttcgccg ccgccagcgt ttcgctgatt tccacaaact
    12841 gttccggctg agcaattgcg ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg
    12901 tcagacgata ggtcgtaccc acatacggga actgctcttt tttacccatc cgcagcgcgt
    12961 cttgttcata cagacgaacc accgggttag aaaccacgtt cggatgcagc gggttagttc
    13021 ccagcggcgt ttcaattggc tcgtagtgtt ccgggaacgg accttccgcc attttgttga
    13081 tggcaaacag gcgtcccatc ccttccggct gcatgataaa cggcccggtt ggcgtacccg
    13141 gtgcggcatt gccgaagtca gggatatcgt tacccgtcca cttgctgccg ttccactgga
    13201 tcagcatccg tttcggatcc cacggtttac cgttgatatc tgccgaagca cggttgtaga
    13261 gtacgcgacg gttaagcggc cacgcccagg cccatcccag cgtattcccc agaccggacg
    13321 ggtcggagtt atcgcggtta gccatctggt tgccctgctc tgtccagcta ccggtgtaga
    13381 tccagcaaga agatgcggtt gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact
    13441 gacctttctt cgcaatcagc acgccattag catcatagag atcttccagc gcatagccgt
    13501 tgttctcttt agctacttcg tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca
    13561 tcttcatcag cggttctacg cctttaccac cttcagcctg gtacagctcg cgcagatgat
    13621 ggtagatacc cgccagaatt tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt
    13681 tccagtgcca ctgcaaccag cgaccggagt tagcgataga accatcttct tctgcaaagc
    13741 aggtcgaagg cagacggaat acttcagtct gaatagacgc cggatcgaca tcgttcgact
    13801 cgccgtggtt ctgccagaag gtagaggttt cagtcaccag cggatcgata accaccatgt
    13861 acttcagctt gctcaggcag ctcaccactt tgtttttgtc cgggaaggac gcaaccgggt
    13921 taaagccctg gcagaaataa ccggtgactt tgccttcatc catcatgttg aaatacttga
    13981 tgacgtcgta ggtctggtcc cacttcggca gccagtcata gccccagttg ttctctttct
    14041 gcgcggcatc gccatagaaa gatttcatca ggctaacgaa gaacttcgga tagttgctcc
    14101 agtagttcac ctgaccagcc agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca
    14161 aatcaacctg tttttctgac ggcagcgtca gataacctgg caggctggta gagagcagac
    14221 ctaagtcagt cagaccctga atgttggagt gaccacgcaa tgcattcacg ccgccaccgg
    14281 ccatacccat gttaccgagc agcaactgga tcatcgccat agtacggatg ttctgcgcac
    14341 ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc
    14401 tggtggaggc cagcacttca cacactttca ggaagtcggc ttttggcgta ccacagatgt
    14461 tttcaacaac gtccggcgtg tagcgggaaa cgtgctcttt cagcaggttc cacacacagc
    14521 gcggatgagt cagtgtttca tcgcgttttg catagccgtt ttcatcgaac tgatagttcc
    14581 aggacgactt atcgtactgg cgtttttcag cgtcgtagcc gctgaacaga ccgtcttcga
    14641 aagcaaaatc atcacgcacc agcaggctgg cgttggtgta atgcttaacg tattcggcgt
    14701 tgattttgtt gttttcgatc aggtagcgca aaacgccaga caggaacgta atgtccgtac
    14761 cggaacgaat aggcgcgtag atatccgcca cagaagcggt acgcgtaaaa cggggatcga
    14821 caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg
    14881 gatgcgcttc agcagcgtta ccgcccatta ccattacgac gttagcgttt ttgatatcca
    14941 cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc aagacttgct accgttggtc
    15001 cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc gagggagcgg gcaaatttct
    15061 gggtcagcat cccggtttcg ttgctggcac cggaagcaca cagcataccg gtagaaagcc
    15121 aacggtttac cgttacgccc tgctcgttct tttcaataaa gttagcgtca cggtcagctt
    15181 tcatcagctt cgcaatacgg gagaatgctt cttcccagct aatgcgctgc catttgtcag
    15241 aacctggcgc acgatattcc gggtagcgca gacggttttc gctgttgacg taatccagca
    15301 aaccggcccc tttcgggcac agcgccccac ggcttaccgg atgatccggg tcaccttcaa
    15361 tgtgataaat cgcttctctg gcgtttttcg cgccatcacc caggctatac atcaatagcc
    15421 cgcaacctac ggaacagtat gtgcaggtgt tacggatctc tttagcgcgt aataatttgt
    15481 agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc caatgccgct actgttgttc
    15541 cagccatacc gcccgcgcag attttaaaaa attgtctgcg actgacgtcc attgctttcc
    15601 tcttttttca ggggtattac tgcgcgagga aactaacagt aaaattctga atctttttgc
    15661 gcgaaatcaa aaagaacacg tgtttgccac aaaataatcc gcctgcgcaa attgacctac
    15721 ctcaatagcg gtagaaaaac gcaccactgc ctgacaggcc agttaaaaaa atgctataaa
    15781 attcagctta atttttaacg gcaagagaga caaaacagcg agcatgacac gacaaaaagc
    15841 aacgctcata gggctaatag cgatcgtcct gtggagcacg atggtaggat tgattcgcgg
    15901 tgtcagtgag gggctcggcc cggtcggtgg cgcagctgct atctattcat taagcgggct
    15961 gctgttaatc ttcacggttg gatttccgcg tattcggcaa atcccgaaag gctatttact
    16021 cgccgggagt ctgttattcg tcagctatga aatctgtctg gcgctttcct tagggtatgc
    16081 ggcgacccgt catcaggcga ttgaagtggg tatggtgaac tatctgtggc ccagcctgac
    16141 aattctcttt gccattctgt ttaatggtca gaaaaccaac tggctgattg taccaggatt
    16201 attattagcc ctcgtcggcg tctgctgggt gttaggcggt gacaatgggt tacattatga
    16261 tgaaatcatc aataatatca ccaccagccc attgagttat ttcctggcgt tcattggtgc
    16321 gtttatctgg gcagcctact gcacagtaac gaataaatac gcacgcggat ttaacggaat
    16381 taccgttttt gtcctgctaa cgggagcaag tctgtgggtt tactattttc tcacgccaca
    16441 accagaaatg gtatttagca cgccagtcat gattaaactc atctctgcgg catttacctt
    16501 aggatttgct tatgctgcat ggaatgtcgg tatactgcat ggcaacgtca ccataatggc
    16561 ggtaggttcg tattttacgc ccgtactttc ctcagcgctt gcag
//
LOCUS       DBFIFS010000090        16508 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.090, whole genome shotgun sequence.
ACCESSION   DBFIFS010000090 DBFIFS010000000
VERSION     DBFIFS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.090"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..345
                     /locus_tag="N7A07_002596"
     CDS             <1..345
                     /locus_tag="N7A07_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2477234.1"
                     /translation="LNDASESDKSKIFFCLSAVFANISHSNVFNGIPDASKTLKRYAF
                     ALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFS
                     KVLSEWTPDAWL"
     gene            629..2779
                     /gene="dinG"
                     /locus_tag="N7A07_002597"
     CDS             629..2779
                     /gene="dinG"
                     /locus_tag="N7A07_002597"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU2477235.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2807..3769
                     /gene="ybiB"
                     /locus_tag="N7A07_002598"
     CDS             2807..3769
                     /gene="ybiB"
                     /locus_tag="N7A07_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU2477236.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3910..4995
                     /gene="hcxB"
                     /locus_tag="N7A07_002599"
     CDS             3910..4995
                     /gene="hcxB"
                     /locus_tag="N7A07_002599"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU2477237.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5062..5121
                     /gene="ybiE"
                     /locus_tag="N7A07_002600"
     CDS             5062..5121
                     /gene="ybiE"
                     /locus_tag="N7A07_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU2477238.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5224..5484)
                     /gene="ybiJ"
                     /locus_tag="N7A07_002601"
     CDS             complement(5224..5484)
                     /gene="ybiJ"
                     /locus_tag="N7A07_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU2477239.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5749..6015)
                     /gene="ybiI"
                     /locus_tag="N7A07_002602"
     CDS             complement(5749..6015)
                     /gene="ybiI"
                     /locus_tag="N7A07_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU2477240.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLC"
     gene            complement(6089..6775)
                     /gene="ybiX"
                     /locus_tag="N7A07_002603"
     CDS             complement(6089..6775)
                     /gene="ybiX"
                     /locus_tag="N7A07_002603"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU2477241.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(6817..9099)
                     /gene="fiu"
                     /locus_tag="N7A07_002604"
     CDS             complement(6817..9099)
                     /gene="fiu"
                     /locus_tag="N7A07_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU2477242.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9364..9624)
                     /gene="mcbA"
                     /locus_tag="N7A07_002605"
     CDS             complement(9364..9624)
                     /gene="mcbA"
                     /locus_tag="N7A07_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU2477243.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            9900..10826
                     /gene="rlmF"
                     /locus_tag="N7A07_002606"
     CDS             9900..10826
                     /gene="rlmF"
                     /locus_tag="N7A07_002606"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU2477244.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10823..13048)
                     /gene="ybiO"
                     /locus_tag="N7A07_002607"
     CDS             complement(10823..13048)
                     /gene="ybiO"
                     /locus_tag="N7A07_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU2477245.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13165..13887)
                     /gene="glnQ"
                     /locus_tag="N7A07_002608"
     CDS             complement(13165..13887)
                     /gene="glnQ"
                     /locus_tag="N7A07_002608"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU2477246.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13884..14543)
                     /gene="glnP"
                     /locus_tag="N7A07_002609"
     CDS             complement(13884..14543)
                     /gene="glnP"
                     /locus_tag="N7A07_002609"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU2477247.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14681..15427)
                     /gene="glnH"
                     /locus_tag="N7A07_002610"
     CDS             complement(14681..15427)
                     /gene="glnH"
                     /locus_tag="N7A07_002610"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU2477248.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15831..16334)
                     /gene="dps"
                     /locus_tag="N7A07_002611"
                     /gene_synonym="pexB"
     CDS             complement(15831..16334)
                     /gene="dps"
                     /locus_tag="N7A07_002611"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU2477249.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         4002 a         4121 c         4386 g         3999 t
ORIGIN      
        1 ctcaatgatg ccagtgaaag tgataagtct aaaatatttt tctgcctgtc tgccgtattt
       61 gcaaatattt ctcacagtaa tgtctttaac ggtattcctg atgcgtcaaa aactttaaaa
      121 cgttatgcat ttgctttatt ggcgaaagct cattctttag atgaaagtat gataagcaat
      181 caaactttta atacatacaa aacggtattg cttgatttca ataatctgtc taatgaagag
      241 gccaaccagt tgcgcataag ttcgttatat cgtgatatgg tccgctatgc acaatatcgt
      301 ttttctaaag ttttatccga gtggacgcca gacgcatggc tgtaaagatc gcagcagaat
      361 aatggcattg atggccctgg ctcaggccag ggtcggcaat accggttcct gcgaagcgat
      421 gacggcggca attgtaacca cgaaatgcgg tatcacttgc tgttttctgc cctgaagact
      481 ggcgctcact gtgccgtcga attttcaatc aaatatattg cgagagggcg tgtttgcgag
      541 ccgctttcca gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc acaatattgg
      601 ctgtttatac agtatttcag gttttctcat ggcattaacc gccgcgctta aagcgcaaat
      661 tgccgcctgg tataaggcgc ttcaggaaca gatccccgac tttattcccc gtgcgccgca
      721 gcggcagatg attgcggacg tcgccaaaac gctggccgga gaagaagggc ggcatctggc
      781 gattgaagcc cccactggcg ttgggaaaac gctctcctat ttgattcccg gcatcgccat
      841 tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcat tgcaggatca
      901 gatctacagc aaagatttgc cactgctgaa aaagatcatt cccgatctta aattcactgc
      961 cgcttttggg cgtgggcgct acgtttgccc gcgtaatctg acggcgcttg ccagcactga
     1021 gcccacgcaa caggatctgc tggcgtttct tgacgacgaa ctgacgccaa ataatcagga
     1081 agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac acttataaat gggatggtct
     1141 gcgcgatcat acggatatcg ctatagatga cgatctctgg cgtcgtttaa gtaccgacaa
     1201 agccagctgc ctcaaccgca actgttacta ctatcgcgaa tgcccgtttt ttgtcgctcg
     1261 tcgggaaatt caggaagcgg aagtggtggt ggcaaaccat gcgctggtga tggcggcgat
     1321 ggaaagcgaa gccgtattgc ctgacccgaa aaatttactg ctggtgctgg acgaaggcca
     1381 tcacctgcca gatgtggcgc gggatgcgct ggagatgagt gccgaaatca ccgcgccatg
     1441 gtatcggcta cagctggact tgttcacgaa actggtcgct acctgcatgg agcagtttcg
     1501 cccgaagacc atcccgccgt tggcgatccc tgaacgtttg aatgcgcatt gtgaagagct
     1561 gtatgagctt atcgcctcat taaacaacat tctcaatctc tacatgcctg ccgggcagga
     1621 agcagaacac cgttttgcga tgggcgaact gcctgatgaa gtgctggaga tctgccagcg
     1681 gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag ttatttctta acgatttaag
     1741 tgagaaaacc ggcagccatg acattgtacg tctgcatcgg ttgattttgc agatgaaccg
     1801 cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg ctggcttcgc tggcgcaatc
     1861 ttccggtgca ccggtgacca aatgggcgac gcgggaagag cgcgaagggc agctacacct
     1921 ctggtttcac tgcgtgggaa tacgtgtcag cgatcagctg gaaaggctgc tgtggcgcag
     1981 tattccgcac attattgtca cctccgcaac cttgcgttcg ctgaacagtt tttcgcgttt
     2041 gcaggagatg agcgggctga aagagaaagc gggcgaccgt tttgtggcgc tggattcccc
     2101 ctttaaccac tgcgaacagg gcaaaattgt tattccccgg atgcgcgttg agccttccat
     2161 cgacaacgaa gaacagcata ttgctgaaat ggcggccttt ttccgtgagc aggtggagag
     2221 caaaaaacat ctcggtatgt tggtgctgtt tgccagcggg cgtgcgatgc agcgctttct
     2281 cgactatgtg acggatttac gtctgatgtt gctggtgcag ggcgatcagc cgcgttaccg
     2341 tttagttgaa ctgcaccgca aacgcgtcgc caacggtgag cgtagtgtgc tggtgggctt
     2401 acagtcattt gccgaagggc ttgatttgaa aggtgatatg ctcagccagg tgcatatcca
     2461 caaaatcgct tttccgccta tcgacagccc ggtggtgatc actgaagggg aatggctgaa
     2521 aagcctcaac cgctatccat ttgaggtgca aagcctgccg agcgcctcgt ttaacctgat
     2581 tcagcaggtt gggcgactga ttcgaagtca cggttgctgg ggcgaagtgg tgatttacga
     2641 taaacgcttg ctgaccaaaa attatggcaa gcgactactg gatgcattac cggtatttcc
     2701 gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa cgaaatcccc
     2761 acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg actatcgcaa
     2821 aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc gggatactgc
     2881 gcgcggtctg tatgcccata tgctcaatgg tgaagtccct gaccttgagt tgggcggtgt
     2941 attgattgcg ctgcgtatca aaggggaagg ggaagcagag atgctcggct tttacgaagc
     3001 catgcaaaat cacaccatca agctgacgcc gcctgcaggc aagccgatgc cgattgtcat
     3061 ccccagttac aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg cgattctcct
     3121 gcacaaactc ggttttcctg tggtggttca cggggttagc gaagatccaa ctcgcgtgct
     3181 gaccgaaacc attttcgaac tgatgggtat tacgccaacg ttgcacggcg ggcaggcgca
     3241 ggcgaagctc gacgaacatc aaccggtgtt tatgcctgtc ggcgcgtttt gcccgccgct
     3301 ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac ataccctggc
     3361 gaaactggcg acgccatttg ccgaaggtga ggcactgcgt ctttccagcg tttcgcatcc
     3421 ggaatacatc gggcgagtcg cgaagttctt tagcgatatc ggcgggcggg cgttactaat
     3481 gcatggtact gaaggcgaag tgtatgctaa tccgcagcgc tgcccacaga tcaatctcat
     3541 tgatcgtgaa gagatgcggg tgctgtatga aaaacaggac cctgctggaa gcgagttact
     3601 gccacaagca aaagatccgg aaaccacggc gcagtggatt cagcgttgcc ttgctggccg
     3661 cgaaccgatt cccgaatcgc tgaaaatcca gatggcttgc tgcctggtgg ctacgggtga
     3721 agcggcaact atcagcgatg gcctggcgcg cgttaatcag gcattttaat tcttttctca
     3781 gccggatgag ccatgtcatc cggcttttac cccaccgcaa tatgaaattc ctgcatcttt
     3841 attgaccttc ccgcgcccgg cgtgcagcat aaaaaaacaa caaacacata acataaacag
     3901 gagttaacca tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag ctttattcag
     3961 gctgtatttc gtcagatggg tagcgaggaa caagaagcga aattagttgc cgatcattta
     4021 atcgcggcaa acctggcagg gcatgattca catggtattg gcatgatccc aagctatgta
     4081 cgctcctgga gtcaggggca cctgcaaatt aaccatcatg ccaaaatcgt taaagaggcg
     4141 ggggcggcgg tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc acatgaagcg
     4201 atggcgctgg ggattgagaa agcgcatcag cacggtattg ctgccgtggc gctacataac
     4261 tcgcatcata tcggccgtat cggttactgg gcggagcagt gtgcagcggc ggggtttgtc
     4321 tctatccact ttgttagcgt ggtcggtatt ccaatggtcg caccgttcca cggtcgcgac
     4381 agccgctttg gcacgaatcc gttctgtgtg gttttcccac gtaaagatga ttttccgctg
     4441 ttgcttgatt acgccaccag cgccattgca tttggcaaaa cccgcgtcgc ctggcataaa
     4501 ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac gaccaatccg
     4561 gcggtaatgc aggagtcgcc gttgggttcg ctgttgacct ttgccgaaca taaaggctac
     4621 gccctggcgg caatgtgtga aattcttggc ggggcgcttt ctggcggtaa aacgacgcat
     4681 caggaaacgt tacaaaccag tcccgatgcc attcttaact gcatgaccac tatcatcatc
     4741 aacccggaac tgttcggcgc gccggattgt agcgcgcaga ccgaagcctt tgccgagtgg
     4801 gtgaaagcct cgccgcatga tgacgataag ccgattttgc tcccgggcga gtgggaagtg
     4861 aacacgcgtc gcgaacggca ggagcagggg attccattgg atgcgggaag ctggcaggcc
     4921 atttgtgatg cggcgcggca gattggtatg ccggaagaga cgttgcaggc tttctgtcag
     4981 cagttagcca gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag ggtaacatag
     5041 gatcaatgag ggttagagca tatgcgtctg tcggcaaaca gacagggaaa tacttgtgca
     5101 ggacgtagcg taaacgcctg atccgtccaa cggttcggat tttgtaggcc tgataagacg
     5161 cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt aaacgcctta tccgtcctac
     5221 gaattactta taaattaccg cagtaccgct taatttattg ttgttggtgg cggaagtgat
     5281 gctatatcca ctggcaccgg ctgccgccgc tttctcagcc agtttcgctt ccagggcatc
     5341 gagggtggat gcgccatcgg cagaaaccac gccgatttta ttcatgttct gtgcctggga
     5401 tgccgttacc ggttccgcgg caaacacgcc aaatgacaga gctgaaagag ccatagcagc
     5461 aacaacagta ttgatagttt tcataattaa tctctcgcag gtgatttttg tataagtgac
     5521 gttgtttcgt cgatgtgatg agtatcacgt ttttttacga gagataaaat cgaagagaat
     5581 tgaccgcctt gttcaaataa attgattgat atctaacata ctgataaacc atttatttgt
     5641 tgtacttatg gacttgtttt atgcagcgtg gcagtcacga cagcgttaaa ggcacggaat
     5701 gccacgcaaa atacaaatta tactcgctat acatctcgcc agttgtagtt aacataactg
     5761 gctttctttc gaacctctgc gattatatcc tgtataagct ggtttttgta aatctttctc
     5821 ctgctgacaa tgaatacata agcgcacgcc aggaatggct tcccgacggg cttgcgggat
     5881 gggggcaccg cactcttcac attcatacag gctttcgcca cgcggaattt caccccgagc
     5941 gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg ttgacggcgt catcgttagc
     6001 ccaaccggat gccatatcgt cctccccata tcaatgcttg tatagttaag tgtagctaat
     6061 atagggacga acctgggcag ttcaagcgtc agatctccga ccattcccgc agcagattat
     6121 gataaagatt gagcagcgac aggatctctt cattttcacc atagcggctt ttcagcgact
     6181 gaatattctg aatatttttg tccagttcaa acagcatggc gcgcttttta tcatcgcgga
     6241 tcatcgactg gatccatata aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc
     6301 aatgcaggct gctggaggga tacaacacca gatcacctgc cgggagtttt acccgatgtt
     6361 gtccgaaggt gtcattaacg accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa
     6421 acagcgtggc agaaaggtca gtgcgcatcc agccgttttg tggatggctg cgtactgcgc
     6481 catccacatg aaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa
     6541 gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct
     6601 cattttgcag ggcggcgtat aacgtgctgc gggtgtctac ctgttgattg ttcttaactt
     6661 gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca ttcggcttgt tccagttgtt
     6721 cgcgaaaacg ggcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaag
     6781 ttctccaaaa agtggggcct gcgccccaca tctgaatcag aaatgcatat tggctgtgag
     6841 caagaaggtt cttggctcgc ccgggtgata acggtagccg ctcttattga ttgaggcgac
     6901 gtaatcggta tcaaacaggt tgtagacgtt tagctggaag tcgagattgc gattaaccct
     6961 ataccccagt ttggcatcgg cgacccagta accttcggta aacgctggcg ttcccaccgc
     7021 gccgtctgaa cctttatgca tactgccgat atagcgtgcg cccgcgccaa cagagatatc
     7081 atcggttgcc tgatattggc tccataaggt gaaggcgtgc tccggggtat acggcagcga
     7141 tgaggaacca tcctgggcaa catctttgcc gtttttgatg gttgcttttt gctgggtata
     7201 gccgccaatc acctgccacg cgggagtgat attccctgcc acggatatct catagccttc
     7261 gacgcgtttc ttaccgtatt gcgagtaagt cccgtcatca ttttgctcaa cttcattttc
     7321 gatatcagta cggaacagcg cggcggtgag caacaggcgt ttatccagaa cctgccattt
     7381 ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt tggcactgtt
     7441 accgctgcca gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct gggaaacggc
     7501 atagttaata tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt
     7561 caccagattg cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc
     7621 agctgggcag gtgatggcac cgcgtccgct gccgccgcag gcggtggcac tgtcatattc
     7681 agtatgataa ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag
     7741 cgtatcgaat gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg
     7801 cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa atatttaccg caggtaacgt
     7861 caccggatta acgccgtagt tagtctgcgt ttcacgggta aattccacgc cggtactgac
     7921 atcatgaccg atagaagcgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat
     7981 tttattactc acatctttgg tattcgccgt gcgtgaccac gtccagctat tcacatcgct
     8041 ggtgggctgg gtaatattcg acgccccacc cataatcgcc gtcatcaggt aatcctgctt
     8101 cacgcgcgac caacgggtag tgttgcgaat ggtggtgtta tcgttgatat cgtgctcaaa
     8161 acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt
     8221 atgcgtatca acttttccgg aatgattcag ggtcgccgtt cctgcagatg gggcagaata
     8281 gcccggcaaa ccgatggttg gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag
     8341 ataattaaga tacaaacgat tcgctgtacc aaggccaaaa gcgatagaag gggcgacgcc
     8401 gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccatcacatt
     8461 caggcgcacc gcagtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa
     8521 ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgcttgct
     8581 gatcatattg atcgagcccg tcggcgcgct gcgcccgtag tcggtgccgg aaggcccttt
     8641 aatcacttcg acctgctcag tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat
     8701 gccatcaata taaatactgt tagaggtatc agcaccgcgc atataaatgg cgtcgccagt
     8761 ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc
     8821 atcggtaagg ttggttgcgc cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt
     8881 gcgggtagta tccgctaccg gacgcgagaa tttcggatcg gcagattgtt gtggcgcata
     8941 aagcgaaggc gttgatgctt cgacaaccag cgtgtcatcc gcgttagttt gcccttcggc
     9001 ggcgagtgcc tgagccacag gcgtgatgcc aatacaaaga ccggcaaaga acgtgagcga
     9061 atgaaattgt ctggcaggga aattgcgatt gttttccatt ttgcaggtga ctttttctta
     9121 tatgttgaaa ttttcactgc cacgagaaag cctctctgcg gatgcgatgc cactgttaat
     9181 ctcgtgatag tgttcgtgca agtgataata attttgataa gcattatcac ttggcgcgat
     9241 tttctatcac ccattgcgcg aaaaagaaat acatttattt acattgcgtt catatttgat
     9301 gttttttgat tacaaaacag gcgaaaactg catccagagg cgaatgcagt tacgttggtg
     9361 gggttatttg tagatggttg cagtaccgaa catctggtca tttccgcctg cggaattaat
     9421 gacataacct ttcgcgcctt gctcgcgcgc tttttctgcc agtttatcct ccagatcgct
     9481 taggtttgat gcgccagttg ccgaaacggt accggcgggc cgtagctgac cgctgtccag
     9541 gttactcatc ggttgcgcgg cataagccgt gagagagatt ccgctcagga cggtggcaat
     9601 cagtagtgtg aggcactttt tcataatgac atcctcataa atacaacggg tagtgcctga
     9661 taagtgtagg tcgcctgcgc gatgcaaatg acgcaaaaaa ttgacagaga tgtgcttatt
     9721 ttttgaacga tatattttta caaaataaga caaatcaatg acatgcataa tgctgatttt
     9781 tatgatgaat tgccataaac catagccatg ttaaggtttt ggggaatgat aaacgcagcc
     9841 agatatttac cctgtcgcgt tatgcgggta tcttacgccg ctgcttaaag gagaatgcta
     9901 tgtccgccca gaaaccgggg ttgcatccgc gcaaccgtca tcacagccgc tacgatctcg
     9961 ccacgctttg tcaggtcaat cctgaactca agcaattcct cacgcttaca cccgccgggg
    10021 agcaaagcgt agactttgcc aatccgctgg cggtgaaggc gctcaataag gcgttgctgg
    10081 cccattttta cgccgtagcg aactgggata tcccggacgg ttttctctgc ccgccagtac
    10141 cgggccgggc ggattatatt catcaccttg ccgatttact ggctgaagca agcggaacaa
    10201 ttccagctaa tgccagcatt cttgatatcg gcgttggtgc gaactgtatt tatccgctga
    10261 ttggcgtaca tgaatatggc tggcgtttta ccggtagtga aaccagcagc caggcgttaa
    10321 gcagcgcgca ggcgatcatc agtgctaatc cggggcttaa tcgcgccatt cgtctgcgtc
    10381 ggcaaaaaga gagtggggcg atttttaacg gcatcatcca taaaaacgag caatacgacg
    10441 cgaccttgtg taacccgcca ttccacgatt ccgccgctgc ggcgcgggca ggtagtgagt
    10501 gtaaacgccg taacctgggg ctgaacaaag acgatgcact gaactttggc ggccagcaac
    10561 aggagttgtg gtgtgaaggc ggtgaggtca cctttatcaa aaagatgatt gaagagagca
    10621 aaggcttcgc gaagcaggtg atgtggttta cttcactggt atctcgtggt gaaaacttac
    10681 cgccgttgta tcgtgccctg acggacgtgg gcgcggtgaa ggtggttaaa aaagagatgg
    10741 cccaggggca aaagcagagt cgctttattg cctggacctt tatgaacgac gagcagcgcc
    10801 gccgttttgt caatcgccag cgttaaagcg ttggctcccc cggcggtaac ggttcagccg
    10861 gggtcgcgcc cggagcaggc agcacctgat aagtctgcac cggcgcgcga acgcccgcca
    10921 ggtcgaaatg ttttttcacc tggctgtcga gggcaaagcg taccgtccac tgtttgagtg
    10981 gcagcgtggt gaacgaaaca cgcagtgtaa acgcggtatt gcttaagcca acaatcccgg
    11041 caaagttcgg ttcaccaata atcagcccac gaatttcttc gttttccatt aattccgcta
    11101 ccgcatcttt cagtgcctga ttggctttat cagcatcttc atggcgatca acatcgtagt
    11161 ttgccactac cgaaccaatg ccgcggacaa agttggcaaa ggtggtaatc gaagaccacg
    11221 gaatgatgtg gtacgccccg gtatcctggc gtacgcccac ggagcgaatc gacatccgtt
    11281 ccacagtgcc ggtcaacggc ccaatagtca ccaaatctcc agtgttcatg ccgttttcaa
    11341 actgaataaa taccccggtg ataatatctt tcaccagcgt ttgcgaacca aacgagatag
    11401 ccagacctaa tgccccggca cctgccagca atggcgcgat attgacgccg atttccgaca
    11461 acacaatcat gatggtgatg gtactgataa tcaccgccag cgcgttacga aacagcgtaa
    11521 gcagggtacg cgtgcgggcg ctgggtagcg ggcggccatg aatatccgaa gccagccggt
    11581 tttcgatcaa actggcgagc accgtccagc caaccgccga gaagaaaaga atgagtgcga
    11641 tacggatcag gatatctacg gttttctgcc ccgcgccgtt ttgcagccag ttccagaaat
    11701 cgaacaatcc ccatgcgctc aacagcagca ttaccgccac gcagacggtc agaatacgcg
    11761 ccgttttcag cgccgccgaa agccagccat tcagccgttt ttgcaactcc ggatagttac
    11821 gctgagtatg tggcgagaga gtgatggttt ttgccagcca gcgggaaaac ataccggaaa
    11881 caaatgctgc gataccaata atcgccaggc tgcgcactgt tgcacccatc ataaatttca
    11941 ggctgttgcc cggatcgaac aacgaaaaga aaaacagcac gataaaatag gcgcttgcca
    12001 gccagtgcca caccagcgca aaggcgcgga taaacaggct gaaaaaggcc agcgaatgct
    12061 ccgcaaagtt gagcaaatgc tgggtaatct cttttttatt acgaaagatc aggtacaacg
    12121 cccagacggt catgcacagc ataatgatga cgttcgccag cgcacctatc tgtacattca
    12181 cctgattaga gataatcggc acggccacaa tcaggccata acctatcaaa ctgcctaacc
    12241 agctcaggcg acgactccag taacgggcgc tctcgtcctg aatcgtgaac gggcgcagtt
    12301 ccgccacgtt tgggcaaaaa atcaggcgta gtacggcttt gaaaaattca atcagggcaa
    12361 aggcgttgag aaacaaactt tgttggaaag cgatggtgcg actgcctgca ttcaggttgt
    12421 cgcttaatat ctggccgaca aataatgtca gtgccagtaa cagcaggtcg ataataaacg
    12481 ccccgataat catcgccgga agctgcagcc agttactgcg ctcacgattt ttttgccgcg
    12541 cccactgacc cattttgcga tacagcggca gtgcgcacag gcgaatcagc cagtaaaaac
    12601 caaacactaa aaccgctaac attgaaaaat gggtcagcgc attgctgaag gtttgtggat
    12661 taaacggctt atgcggggag ccggtgatat tgcgataaag ctgcccgaag cgggcggaaa
    12721 gggcttcacc ataatggcgg ctgaattcgg tgaccttttg cagcacggtt tgctcttcaa
    12781 ccagcgtcgg cggcacgatc ttcgggaccg gttcagcagg tggcgtcgcg gcgacggtgc
    12841 gcaactggtc gatcaactct ttacgcgagg tgtcattatc cagcacatcc gccagtgcgc
    12901 cataagccgc ttttttttgt tcgatatccg gttccggggc cggttcagtc gttgagtccg
    12961 ttgtcgttgt ggttgtaacg ccgggtatgg ataccgcgtg ggcaggtgcg cccagcaggc
    13021 agaagaggat gaacaggatc caccgcatga ctcctccagt gagaaaacag agcaaaaaga
    13081 taagtataga tgctggggga ggggtgattt taaatgagag gaatctggtg tacctccctt
    13141 tcgggtgaaa gggaggcaga gaagttaaga gacgtgctgt aaaaattcct gcaagcgctg
    13201 gctcggcgga tttttaatca acacttgtgg atcgccatct tccgcaatcc ggcctttgtc
    13261 gataaagatc aggcgcgaag ctactttctc ggcaaaaccg atttcgtggg tcacgatcac
    13321 catcgtcatc ccttcttcag ccagatcttg cataaccttc agcacttcat ggcgcagttc
    13381 cgggtcaaga gcggaagtcg gttcatcaaa cagcatcatt ttcggcttca ccgccagcgc
    13441 gcgggcaatc gccacacgct gctgttgacc accagaaagt tcggaagggt agtgatgtgc
    13501 acgttctgcc agaccgactt tcgccagcag ctcacgtgcc agtttttccg cctcttcttt
    13561 gttcgcgcca cgcacgcgta gcgggccaaa catgacgttt tccagcgccg tcagatgcgg
    13621 gaagaggtaa aactgctgga acaccatacc tgcttcctgg cgaatcaggc gctcgtcaac
    13681 tttcggatcg ttaaccttca ggccatcgac aatcagatcg ccggaggtga tttcttccag
    13741 tttgttgatg cagcgcagca gggtcgattt accggaaccg gacggcccga taatcaccac
    13801 gacttcgccc tgggcaatgt tcaaatcgat attgtgcagc acctgggttg ggccaaagtg
    13861 cttggagacg tttttaaatt caatcacagg attttcatcc ttctttccag acgacgcaga
    13921 ataaagctca gcaccagggt aataatcaga tagaacaccg ccacggcgct ccagatctca
    13981 agggcgcgga agttaccggc aataatttct tgcccctgac gggtcagttc cgccacgccg
    14041 atcacaataa acagcgaggt gtctttaatg ctgatgatcc actggttacc cagcggcggc
    14101 agcatacgac gcagtgccag cggtaaaatg acgtagcgaa tggtttccca acgtgaaaga
    14161 ccgagcgcca gtcctgcttc acgaaaacct ttgtggatag acagcaccgc accacgcgtg
    14221 atttccgcaa tgtacgcgcc ggagttgatc atgatggtga ccaccgccgc agtaaatggg
    14281 tcgatgcgta agtcattaaa cgccatcggc agggcaaaat agataaacat cacctggacg
    14341 acgataggtg tgccgcggat cacttcaata aaaaccagcg cgacgtggtt ggctatccaa
    14401 ccaccgaagg tgcgtgcaaa acctgccagc aatccgatca ccaggccgcc agccagaccg
    14461 aggaccgata tccacagggt cattttggca ccttcaatca gaagcggaat ggcaggccag
    14521 atagcactcc agtcaaactg catatgttgt tcctgttacc gtggtgtaaa taatcaaatt
    14581 cagggtaatt aatggccccg gagcgggttt gcgccctcac cctaatcctc tccccagagg
    14641 ggagagggaa ctgccagtgc gctttacagg tgtagcgtta ttatttcggt tcagtaccga
    14701 accatttttt gtagatttcg ttgtaagttc cgttctcgcg cagggttttc aacgcgccgt
    14761 tgactttgtc acgcagctcg tcgctacctt tcgggaacgc aataccgtat tgctgcgctt
    14821 ccagagagtc gcctaccgct ttgaactgac cgttaccggc ggttttgatg aagtacagaa
    14881 tgtttggcgt atcgtgcaga acggcgtctg cgcggttggt gcccagttcc atataggcgt
    14941 tatcgatgtt cgggaactga cgcagatctt tagttttgat gtttgctttc gcgtaatcaa
    15001 cggagccagt gccgctcttc accgcaacca ctttcccgtc gagatctttc acgcttttca
    15061 catcgttatt gttagctttc accatcacta acaggccgct tttgtagtag ccgtcagaga
    15121 aatcgatcgc ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc agatcgacgt
    15181 ttttggtttg cagtgccgga atgatcccac tgaaatccat cggcttcagt tcgtaatcca
    15241 gcttcagctc tttagcgatg gcagcccaca gatcaacgtc aaagcccaca tatttatcgc
    15301 cctgtttaaa ttcaaacgga acgaaggcgg tatccgtcgc gacaactaat tttttatccg
    15361 cggcatgaga agaaaccgca aaagccaggg tcagtgcagc cagtgaaact tttaatacag
    15421 acttcatagc atttcctttt ttctatccac gggacgatcc cctgcgtgac attcacatat
    15481 atgaaaaaat cgtgccagtt ttgcaactca ttgatttttc gagatgcggc aaaaacgtga
    15541 tgcacgattt atagggcaac accctgaaga tgcaccattc tggggcacca aactggtgcg
    15601 ctaaaattgt gcactcaaca cagtgattat ttagcgtaaa acctgatgaa aaaacaatct
    15661 ttatgtaacg attgtgtgat gatgtggata caaaaaattt acttaatcag ctggggatag
    15721 cagatggatg cactaaataa gtgcgttgag gcgggctgca aaacaaaacg gcctcctgtc
    15781 aggaagccgc ttttatcggg tactaaaatt ctgcaccatc agcgatggat ttattcgatg
    15841 ttagactcga taaaccacag gaatttatcc aggtcgcgag acgcggcggt caggatatct
    15901 gcggtgtcgt catctttcgc ttcgccaatc gctttgcgta cgtcattagc gacgattgcg
    15961 taacggtcag ccagttcttt caggtgatcc tgaacgttgt ggatgtccag cgggtaactt
    16021 ttcagcgggg ttttgctgtt gataacttga gtggtcccca gagctacacc gcccagctgc
    16081 actgcacgtt ctgccatggt atccagatga tcgatcagtg cagtgcggaa gccatccagc
    16141 atttcatgta cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg tttggtaatc
    16201 aaagaaagat caataaactg gataacctgg cgattcagca actctactgt tgcttttttc
    16261 tcgctgtcag agacatcgtt gcgggtataa agcagattgg tcgcttttga tttaactaat
    16321 ttagcggtac tcataatttc atatcctctt gatgttatgt cccagtaatt aacgagacta
    16381 agtatagcac cggctatgtg ttccgctatt ctggctgttc ctatcacact aatagtggta
    16441 acaagcgtga aaaacaaaac taataaagat tcaatgagtt agatatattg ataagaacaa
    16501 ttcttatt
//
LOCUS       DBFIFS010000091        16495 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.091, whole genome shotgun sequence.
ACCESSION   DBFIFS010000091 DBFIFS010000000
VERSION     DBFIFS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.091"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            207..1232
                     /gene="dgcP"
                     /locus_tag="N7A07_002612"
     CDS             207..1232
                     /gene="dgcP"
                     /locus_tag="N7A07_002612"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU2477250.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            1265..1363
                     /gene="yoaK"
                     /locus_tag="N7A07_002613"
     CDS             1265..1363
                     /gene="yoaK"
                     /locus_tag="N7A07_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU2477251.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            1366..1440
                     /gene="yoaJ"
                     /locus_tag="N7A07_002614"
     CDS             1366..1440
                     /gene="yoaJ"
                     /locus_tag="N7A07_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU2477252.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(1499..1747)
                     /gene="yeaQ"
                     /locus_tag="N7A07_002615"
     CDS             complement(1499..1747)
                     /gene="yeaQ"
                     /locus_tag="N7A07_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU2477253.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1894..2076)
                     /gene="yoaG"
                     /locus_tag="N7A07_002616"
     CDS             complement(1894..2076)
                     /gene="yoaG"
                     /locus_tag="N7A07_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU2477254.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(2080..2439)
                     /gene="yeaR"
                     /locus_tag="N7A07_002617"
     CDS             complement(2080..2439)
                     /gene="yeaR"
                     /locus_tag="N7A07_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU2477255.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(2612..3250)
                     /gene="leuE"
                     /locus_tag="N7A07_002618"
     CDS             complement(2612..3250)
                     /gene="leuE"
                     /locus_tag="N7A07_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU2477256.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(3377..4300)
                     /gene="dmlR"
                     /locus_tag="N7A07_002619"
     CDS             complement(3377..4300)
                     /gene="dmlR"
                     /locus_tag="N7A07_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU2477257.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            4403..5488
                     /gene="dmlA"
                     /locus_tag="N7A07_002620"
     CDS             4403..5488
                     /gene="dmlA"
                     /locus_tag="N7A07_002620"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU2477258.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            5739..7349
                     /gene="yeaV"
                     /locus_tag="N7A07_002621"
     CDS             5739..7349
                     /gene="yeaV"
                     /locus_tag="N7A07_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU2477259.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            7381..8505
                     /gene="yeaW"
                     /locus_tag="N7A07_002622"
     CDS             7381..8505
                     /gene="yeaW"
                     /locus_tag="N7A07_002622"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU2477260.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            8561..9526
                     /gene="yeaX"
                     /locus_tag="N7A07_002623"
     CDS             8561..9526
                     /gene="yeaX"
                     /locus_tag="N7A07_002623"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU2477261.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(9580..10695)
                     /gene="rnd"
                     /locus_tag="N7A07_002624"
     CDS             complement(9580..10695)
                     /gene="rnd"
                     /locus_tag="N7A07_002624"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU2477262.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10777..12462)
                     /gene="fadD"
                     /locus_tag="N7A07_002625"
     CDS             complement(10777..12462)
                     /gene="fadD"
                     /locus_tag="N7A07_002625"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU2477263.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(12667..13248)
                     /gene="yeaY"
                     /locus_tag="N7A07_002626"
     CDS             complement(12667..13248)
                     /gene="yeaY"
                     /locus_tag="N7A07_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU2477264.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(13288..13983)
                     /gene="tsaB"
                     /locus_tag="N7A07_002627"
     CDS             complement(13288..13983)
                     /gene="tsaB"
                     /locus_tag="N7A07_002627"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU2477265.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(14041..15951)
                     /gene="yoaA"
                     /locus_tag="N7A07_002628"
     CDS             complement(14041..15951)
                     /gene="yoaA"
                     /locus_tag="N7A07_002628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU2477266.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            16083..16427
                     /gene="yoaB"
                     /locus_tag="N7A07_002629"
     CDS             16083..16427
                     /gene="yoaB"
                     /locus_tag="N7A07_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU2477267.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4052 a         4305 c         4045 g         4093 t
ORIGIN      
        1 cagaacaaaa ctgataattt tcatttattg atctcacata tgtatccaag cttagagtat
       61 cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa catattacgt
      121 cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat aatcagcatt
      181 tacccgccgt atcctggagt tgtttcgtgt cagatcagat tatcgcccgc atctcgcaat
      241 cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag atgctggaaa
      301 tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc
      361 atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt accgtctcgt
      421 gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc gatgaagtcc
      481 ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca tttctgagca
      541 cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc agcagtgaga
      601 agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga ctgattgcac
      661 aatatattca aaaagaggca ctggttgaac agctgcgcga agccaatgct gcgctgattg
      721 cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt tttgaaaatc
      781 tgacgacgct gttttccctc gcccggcatc ttaaccaaaa gataatgatc gcgtttatcg
      841 atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc gatctgtttc
      901 tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc
      961 tcggcggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat atttcgtcgc
     1021 tgcgagaacg cattcagcag caaatacgcg gagaatatca cttaggtgat gttgatttgt
     1081 attatcctgg tgccagtctt ggcatagtag aagtcgatcc tgaaacgacc gatgcagaca
     1141 gtgccctaca tgctgccgat atcgcgatgt atcaggagaa aaaacacaaa cagaaaacac
     1201 cttttgtcgc gcattcagcg ccacattcct gaggcgtatt cacatccttt tgattggtga
     1261 taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg tcgtattttt
     1321 agcatggttt tttattggcg gctatgctgc cccgggagca taaagatgaa aaaaacaacg
     1381 atcattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg atggtggtaa
     1441 cgtcacatct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa tttacgcgtt
     1501 aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg gcaaccacga
     1561 agctgccaaa attgaagcca tcgactttac caaagccaaa cagcgtgctg atccaaccgc
     1621 cgactacggc accgactatc cccagcagga tagtcataaa gaatccacca ccatctttac
     1681 ctggcatgat ccacttcgcc agaataccgg caataagccc aaaaataatc catgacagaa
     1741 tgcccattgt ttcctcactt atctgttttg cattagcggg ttagtcgctg ataaaaagca
     1801 tagcacaaca tcgggagggc aagatttgtg acgagcatca cggaggtttt tttgcgatgg
     1861 cgcagaaatt gcgccatcaa cgatcagtga taattaccaa ccacaaacat catgttcgtt
     1921 ttccgtgtca taagaacgca cggtattcac cagatctttt atcacttcag ccgccacttc
     1981 tggcaccagc aaagtcatcg gcgtctctgt ttcataatcg acagaaacgc cattgctgtt
     2041 attggtgacg gtcacggtat acgttgcttt gcccatgatt catttcccgt tatgaatgac
     2101 tttccgttgt tgcgcacctt ccatcaggac ttcaggagcc acgaagaagt caatgttgaa
     2161 ataagtatcg tcagtcatgg cttcaatgtt gtgccacttt tctggaggga acaccgcaaa
     2221 ctgccccgct tcgataagga tcacctgatc aggctctgca ctgtgttcat cagcgtagcc
     2281 gagatatttg accgccccat gcataacgga aaggcgtggg taaacccccg ggcgcgttcc
     2341 tttatcaaga tgacgttcga atattccggc aggcgcagtt tgtttattcc agaaaggcgt
     2401 tgagcgcgta tgaatataat tctgtgggat ttgcagcatc cttttccctc cttcggtgaa
     2461 tgcgctgaaa acggtttatt ccagccgttt cagggtacgc ctgataattt gctttttaaa
     2521 taccatttat tggttacttt ttagcaccat atcagagaaa aatcagggag gattatagat
     2581 gggaagcccg cgacaacgcg ggctgaaagc atcaggattg cagcgtcgcc agtcgggcag
     2641 cgaaacccac gaacatcaaa ccaatcagtg agttgcccac tttagccagt ttctttttgg
     2701 tacgtatgta ctgcgtgaca aaagcaccag atataatcag gaagctcaaa tagcagaaac
     2761 tcaccagttc cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa
     2821 catcgataaa ctgtacgaaa aacgacacat agaacaaaat ggctttcgga ttagtcaggc
     2881 tcaaaattaa cgcgcgttta aaaatagcac cgtattgggg ctcatcggat ttggcctcgt
     2941 tatttttacc cttcagggtc gcgtaaagaa ttttactccc cagatagagc aaataaaacg
     3001 caccaagata acgtacaatg ttgaataata tcggggtggt cttaattaat gtcgccattc
     3061 cagcccatgc cagaaacatc aataccgcat cgccaataaa tacaccgcag gccgcaagat
     3121 aaccgccttt cataccgcta ctgacgctat ttttgagtac aaacagggta tttggccctg
     3181 gcaccaacac aataaaaatg gccccaacca gataggtcca gtaattcaga accccgtatt
     3241 cagcgaacac attaacctct ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg
     3301 ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt tttaacctga tatcaacccg
     3361 ataattgaat cattaatcag gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat
     3421 actggcgtaa aaattctacc gttatccgca ctttcgctga cgtcgccagc cttgaaacat
     3481 aaacggccca gacgttcgct ggctggtaat attccggtag cacttgcact aaatgaccac
     3541 tggcaatgtt ttcgctaaca tcccaccagg aacgcagagc aatcccctgc ccatcgagac
     3601 accactgatg gacaatttct ccatgattag acgacagtgg gccggtgact ttaatcgcat
     3661 ggtggccttc tttattgcgt aattgccaga caccaaaagg atgatcgcgc tctttaatga
     3721 ccaggcacgg caatgcagaa agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa
     3781 attccggcga cgcgcagaga atgcggtaat tggtcgccag tttgcgggcg attaaattag
     3841 gggcgatgtc atcgccaatg cgaatatcga gatcgacacc ttcattgacc aaatcgacca
     3901 gccggtcttc cacatcaaaa cgtaattcca gttgcggata cgccttcgcc agcgctgata
     3961 acgccggagc caccacctgt cgtccaaaac caaagctgct gataatacgc agcatcccct
     4021 gtggcacctg acgcacgtca gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc
     4081 gctgcgccca ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc
     4141 ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg
     4201 ccggtgaaac gcccagttct tccgccacgg cggcaaaacc agcccggcga gccaccagca
     4261 taaagacgcg caaatcattc agcagcggta aattattcat gattcgtgtt ttatgtttca
     4321 ccagttacgg ggattaattc cttttcagtc aattatagga tggtgatgtt gtcaattttg
     4381 atggtcagga agtgagaacc caatgatgaa aacgatgcgt attgctgcga tcccgggaga
     4441 cgggattggc aaagaagtcc ttcctgaagg gattcgcgtg ttacaggctg ccgctgagcg
     4501 ctggggcttc gccttgagtt ttgagcaaat ggagtgggcg agctgcgagt attacagcca
     4561 tcacggtaaa atgatgccgg acgactggca tgagcaactt agccgtttcg acgccatcta
     4621 ttttggtgcc gtcggctggc cggacaccgt tccggaccat atttcgttgt ggggttcgct
     4681 gctgaaattt cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc gtctctttcc
     4741 tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac atcgattttt acgtggtcag
     4801 ggaaaacacc gaaggcgaat attcctcgct cggcggtaga gtgaatgaag gtacagagca
     4861 tgaagtcgtc attcaggaat cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta
     4921 tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta acttctgcca ctaaatcaaa
     4981 cggtttagcc atcagcatgc cgtactggga tgagcgagtg gaagcaatgg ccgagaatta
     5041 cccggagatc cgctgggaca agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca
     5101 gccggaacgc ttcgatgtgg tggtggcgtc caatttgttt ggcgatatcc tttccgatct
     5161 tggcccggcc tgcaccggca ccattggcat tgccccatcc gccaacctga atccggaacg
     5221 cactttcccc tcgctcttcg agcctgtcca cggttccgcg ccggatatct acgggaaaaa
     5281 tattgctaac cctatcgcca caatttgggc cggggcaatg atgctcgatt ttctcggcaa
     5341 tggcgatgag cgtttccagc aagcgcataa cggtattctg gcagcgattg aagaagtgat
     5401 tgctcacggg ccgaaaacac cggatatgaa aggcagtgcc accacgccac aggttgccga
     5461 cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg
     5521 acgcggtttt gttattcgtt ctttgcagtt aataacctgc gtcatttcac cttttattgt
     5581 ttccgtttcg tgttttatgg ctttccgtat tcttaattgt ttaatttatg taacatgcaa
     5641 ataatcaatt cgttaaacac gacatacaac tcattaccct gcaaataatt acaactggca
     5701 aaagccagag acgggctttt atgcccagga gatgactcat gatgagcaat gttaagaaaa
     5761 aagatgtgcc gctgataagc atcagcctgg tggccattct tttcatcgca gctgcattaa
     5821 gccttttccc acaacaatcg gccgacgcgg ccaacgcaat atacactttt gttacgcgta
     5881 cgttaggttc cgcagtacag gtattggttt tgctggcaat gggactagtg atttatttag
     5941 ccaccagtaa atacggcaat attcgtcttg gcgaaggaaa accggaatac agcacgctct
     6001 cctggctgtt tatgtttatt tgtgccggtt taggttcttc tacgctttat tgggggggtg
     6061 ctgaatgggc ctattattat caaacgcctg gattaaatat cgcaccgcgt tcacaacagg
     6121 cactcgaatt tagcgtccct tactccttct tccactgggg catcagcgcc tgggcaactt
     6181 atacgctggc ctcattaatc atggcttatc actttcatgt gcggaaaaac aaaggtctga
     6241 gtctttccgg cattattgcc gcgattaccg gcgttcgccc gcaaggccca tggggaaaac
     6301 tggttgattt gatgttcctg atcgccactg tcggcgcact gactatttcc cttgttgtca
     6361 ccgcagcaac ctttactcgt ggactttccg cactgaccgg tttacccgat aacttcaccg
     6421 tgcaggcatt tgtgatcctg ctttccggcg gcattttttg cctaagctca tggattggta
     6481 tcaacaacgg tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat
     6541 tactggtgct gattgtcggt ccaaccgaat ttattaccaa cagcatcatc aatgccatcg
     6601 gcctgaccac gcaaaacttc ctgcaaatga gcttattcac cgatccgctt ggcgatggtt
     6661 catttacccg caactggacc gttttctact ggctgtggtg gatctcatac acccctggcg
     6721 tagcaatgtt tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt atctggggac
     6781 tgatcctcgg cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa agctatgcca
     6841 ttcatcagtt tatcaatggc gtaatcaacg tcccacaggt gctggaaact ctgggcggcg
     6901 aaacagctgt acagcaagtt ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat
     6961 acctgggcgt gatgattatt ttccttgcct cgcatatgga tgcagtggcc tacaccatgg
     7021 cggcgaccag tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt
     7081 tctggtgcgt ggtgatcact ctgatcccgc tttccatctt gtttaccggt gcttcgctgg
     7141 aaacgatgaa aaccaccgtc gtgctcacag cccttccctt cctcgtcatt ttactggtga
     7201 aagtcggcgg atttattcgc tggctgaaac aggattacgc cgacattccg gctcatcaag
     7261 ttgaacatta tctcccgcag acaccggttg aagccctgga aaaaacacca gtgctccctg
     7321 cgggaaccgt attcaaaggc gacaactgag cgccatcatc ctaacgataa aggtatccct
     7381 atgagcaatc tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag
     7441 gcgtggacca ttcctgcccg tttttatacc gatcagaacg cctttgaaca cgaaaaagag
     7501 aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca gcgaactggc gaatgccaat
     7561 gattatgtga cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag
     7621 gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa
     7681 ggaaaagcaa aaaatgtgat tacctgccca tatcacgcat gggcattcaa actcgatggc
     7741 aatctggccc atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa
     7801 ctggttccgg tgcgtctgga agaatatgcc ggattcgtct tcatcaacat ggaccccaac
     7861 gccaccagcg tagaagatca gttacccggt ctgggcgcga aagtgctgga agcctgcccg
     7921 gaagtccacg atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag
     7981 aacattgtcg ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc
     8041 gactccgtac aggttgatcg ttactggcac accatgcacg gtaactggac gctgcaatac
     8101 ggtttcgcca aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc
     8161 cacggtttct ggctgtggcc gtgcacgatg ctgaatgtca ccccgatcaa agggatgatg
     8221 acggtcattt atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac
     8281 ttcaccaatg aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg
     8341 ttccgtccgg aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc
     8401 tatcgtggtc aggggcgcat catggccgac agtagcggta gcggcatttc cgaacatggt
     8461 atcgcccatt tccataatct gctggcgcag gtatttaagg actaatgaca tcggcggcgg
     8521 tattttccgc cgccgggctg atttttgatg gagtacagca atgtcagact atcaaatgtt
     8581 tgaagtacag gtgagtcagg tggaaccgct taccgaacag gtgaaacgct tcacgccggt
     8641 ggcaaccgat ggcaaaccgc tgcctgcgtt caccggagga agtcacatca tagtgcagat
     8701 gagcgatggt gataaccagt acagtaatgc gtattcacta ctgagttcac cgcataacac
     8761 ctctagttat cagattgccg ttcgactgga agaaaactcg cgcggcggtt cccgcttttt
     8821 gcatcagcag gtaaaagtgg gcgatcggtt aacgatctca acgcctaata acctgtttgc
     8881 gctaattccc tcagccagaa agcatctgtt tatcgcgggc ggtattggta tcaccccttt
     8941 cctgtcgcac atggcagagt tgcaacacag cgacgtcgac tggcagctac attactgctc
     9001 gcgaaatcca gaaagttgcg catttcgtga tgagctagtc cagcatccgc aggctgagaa
     9061 agtccatttg catcattcat caaccggaac acgactggaa ttagcgcgat tattggcgga
     9121 tatcgaacct ggcacacacg tttatacctg tggccccgag gcgctaattg aagcggtaag
     9181 aagtgaagct gcgcgtctgg acatcgctgc cgatacgctg cactttgagc aatttgctat
     9241 cgaagacaaa accggcgatg catttaccct ggtgcttgcc cgttccggaa aagagtttgt
     9301 ggtgccggaa gagatgacta ttttgcaggt tattgaaaat aataaagccg cgaaagtgga
     9361 atgtttatgt cgtgaagggg tatgcggaac ctgcgaaacg gcaatactgg aaggtgaagc
     9421 tgaccatcgg gatcagtatt ttagcgatga agagcgtgcc agccagcaaa gtatgttgat
     9481 ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg ccgaagccgg
     9541 atgtggcgct gagcgcgccc agtccggctt cggaaaattt tactgcggat attcctgcaa
     9601 taaattgtgt aatgcttccg ccatcagctc accacgccag ccggaaatca gctccggcaa
     9661 attgttctgc ggtttcagtt tccagtgcca gttcagcagt tggttgattt gccgacgcga
     9721 tgccagcaat tcggcgctga tcttgtgcgt ttcgctcacg tcagtaatca gcgacttaat
     9781 cgctttaaac gctttacgat aacccggcat gtccatcagg ttaagcatcg gctgcggtaa
     9841 ggcctcttcc ggcaatgtct gcgctttttc caccagcgcc agcagcgttt taccgtgaaa
     9901 gcggatttcg ctaccggata aacccaggct gtccagttcg cctaaacttc ccggcatata
     9961 acgcgctacc gaccacaaat gctcttcacg cacgacaaag ttcaccgcca gatcgcgctc
    10021 tcgcgccttg cgcagtcgcc agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg
    10081 taattgccag gcattggtga tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg
    10141 acgcatttgc atcaggcgac attcatccag cgccgctggt agccagccgg aggcctccgt
    10201 ttctaccata agcttggcgg tgatcggtaa cagataccag acatccgccg ctgcatattc
    10261 acactgacgt tcggtcagcg gtctggccag ccagtcggtg cgcgattcac tcttgtccag
    10321 cgtaacgccg gaatactctt ccaccatgga agcgaaaccc catgacatcg ggcgtccgca
    10381 gaaggcagca aggatttgcg tgtcaatcag gggttgtggt aattcgccaa agacattgag
    10441 gaacacttcc agatcttcac tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag
    10501 gatcgctttc agcggtgacc agtcggtgat cccgagagga tcaattagcg ccagatgctc
    10561 gccatcgaaa agttgaatca accccagctg cgggtaataa gtgcgcgtac gaacaaattc
    10621 agtatccagg gctatcgccg gaaaggcacg gacggcttca cacaaagaag ccagcgcatc
    10681 gtccgtggta atcatttggt aattcaaatt gttttctctt tagtgggcgt caaaaaaaac
    10741 gccggattaa ccggcgtctg acgactgact taacgctcag gctttattgt ccactttgcc
    10801 gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc
    10861 atcacgaaac tccaccagct tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt
    10921 caccagtgac tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc
    10981 accactggag ccggaaggta caccaacagc cgcgacttcc tgtacgccag gatgctgcat
    11041 gacgacatct tcaatctcgt tgggatagac gttaaaaccg gaaaccaaaa tcatgtcttt
    11101 tttacgatcg acaatgcgca ggaatccttc ttcatccatt accgcgatgt cgccagtgtg
    11161 taaccagccg tttttgatga tttcatcggt agcatccgga cgctgccaat agcccagcat
    11221 cacctgtgga cctctgacgc aaagttcacc tggctgacct ggcgatactt cattatcgtc
    11281 gtcatccacc agtttggctt ccgtcgacgg caccggcaaa ccaatgctac cgctatgata
    11341 atcaatatca taggggttca cgctgaccag cggcgcacat tcggtcaggc cataaccttc
    11401 cagcagatac tgtccggtca gtttcaccca gcgttctgcc accacttgct gaacaggcat
    11461 cccaccgcct gcagaaaggt gcagactgga gaaatccagt tgttgaaact ctttattgtt
    11521 cagcaacgcg ttgaacaagg tgtttacgcc ggtgatagcg gtaaacggat atttcgccag
    11581 ctctttgacc agccccggaa tatcccgcgg gttagtgata agcaggttct gcccacccag
    11641 ttcgataaac agcaggcagt taatggtcag tgcaaaaatg tgatacagcg gcagcgccgt
    11701 caccaccagc tctttgcccg gatgcagcag aggcccatac gtagcgttaa cttgttccag
    11761 gttcgccagc atattgcggt gagtcagcat cgcgcctttc gccacacctg tggtgccgcc
    11821 agtgtattgc aggaaggcta aatcttctgg caccagttca ggtttgacat actgcatacg
    11881 gtagccgttg tgcagggcgc tacgaaatga aatggcatct ggcagatgat atttcggcac
    11941 cagacgtttg atgtatttaa caacgaagtt aaccagtgtg cctttcgctg tggaaagctg
    12001 atcgcccata cgggtcagga taacgtgctg aactgcggtt ttatcgacca ctttttccag
    12061 cgtgtgagca aagttggaca caatgacaat cgccgatgcg ccgctatcgt taagctgatg
    12121 ctcaagctca cgcggggtat acaacgggtt aacgtttacg acgatcatcc cggcacgcaa
    12181 aatgccaaac agcgccaccg gatattgcaa taaattaggc atcatcaacg caacgcgatc
    12241 gcctttcttc agccccaacc cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc
    12301 cagcttgcgg aaggtcatca cctcccccat attcacaaac gcaggctgat cggcgtagcg
    12361 cgcgaccgac tgctcaaaca tatctaccag agattgataa cggtcagggt tgatctccgt
    12421 cggaacgtcc gcgggataac ggttaagcca aaccttcttc aattcttcac ctctaaaatg
    12481 cgtgttcgtc gtcatcgcaa ccccaaatga tatacatgcc gttaacataa tattaactca
    12541 tcataccagc ttgataatta cccaacgaaa aggttgcgaa gcgcgtcact atttattttt
    12601 atctttaccg taagaatgca gaaacagcgg accagccgct gtttcttttt ctttaaaaac
    12661 aagcaattat tcagttacaa ctgtttgtac tctcgcgggg ccgggattat accagcccca
    12721 tccgccgtgg ccatagggcc agccacgacc gccataaaac cagggatcaa tcggctgagg
    12781 cggcataatc acctgctggg ttaaatgcca acgtttgtaa cctgttgcct gcatcaccat
    12841 aaatttatag ggcgtgttgc cgatttttcc gtcaaccgca ccagtgattg gcccgactac
    12901 cgtaaccagt tgtccacgga aatccaccgg gtccagaaaa ccgttcacat cggcataaat
    12961 gcgaccgcga gaaggttctc ccagcgtcgg tctggctccg ctgtccagcg gtacggtagc
    13021 aatttccagg cgggttttcc cttgctggtt ttgtaccgca accactttgc caccaaagcg
    13081 tgcctcctga ccaacgtaca gctgcggcgc actcatcacc cgaactaaat cttgttgcgg
    13141 cgtgggactg ctgcctttaa tggcgtccgg cacagtgaca caaccgctca gcattagcgc
    13201 aaacgtacct gccagtatgc ctttgataac attcttttga accgccatga tgcgactcct
    13261 ttttttctca gggcatactc ttaagattca ttctttgccc ggaagtttct tccatgcgac
    13321 gttgttacgt aaataaaccg gttcggcatg ttccaccgcc accgttttac cctcggcaaa
    13381 catctgacaa gcaatcggca gcatatcttc tgcagcgggc agtaaaactt ctccgtcgcg
    13441 caaaaccagt ccgctctctt taccgagatc cggccaggct tgccagcctg tacctaccgt
    13501 cacccattcc ccggaaagct gttgcattcg ttcatgaacg agttcgggtt tgagtacggc
    13561 ttcggtttct tcaccgtgcc agataccgtt ttcatcacgc tgatattcgg cccagtaaac
    13621 ttcgcccatt cgcgcgtcaa tggctgacag cacgcgtgtt gcgccgtttt tgcgccacgc
    13681 cccttgcgcc atcgtcatta gcgtggagac gccaatcatc ggtaattccg cgccaagcgc
    13741 cagcccttgc gcgatgccaa taccaatgcg cacgcctgta aagctaccgg ggccgcgccc
    13801 gtaagccaga gcgttaatat cagtcaggga agttccgctg gtggtcagga tatcctgcac
    13861 catcggtaag attcgttgag tatgttcacg agggcaaagc tcaaaatgag cgttgacagt
    13921 accgtcgttc cacagggcga cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg
    13981 catgggtctt cgtgcttaga tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa
    14041 ttacctggag gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac gggtgcgtgg
    14101 cgcgggcggc agactggcga gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc
    14161 acaaattacc aaaaccccgc gatcgtcggc gtcgcgaatc agtcgcccga ctccctgctt
    14221 gagagtaatc accgcatccg gcagttgtac ttcatcgaac gggtcgccac cgcgcaaacg
    14281 gcaatcttcc atgcgcgctt ttaacaatgg atcatcaggc gaggtaaacg gcaatttgtc
    14341 gataatcacc aatgacaatg tatcgccacg cacgtccacc ccctcccaga aactactggt
    14401 tgccacaaga agcgcgttac cggcactgac aaattgctgc aacagttgcc ctttgctggt
    14461 ttccccctgc aataatacgg gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg
    14521 catcatggcg tgcgaggtac aaagcataaa acagcgaccg ttgttagctt cgatgatcgg
    14581 gcgcagcata gccgccagtt gccgcgcgga acccggttga ttggtttgcg gcaggttgcg
    14641 cggcacacag agtaacgcct ggcggctgta atcaaacggg cttggcaata gcaacgactc
    14701 cgcctgttct atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttta ccgacagcgt
    14761 tgctgaggtg aagatccagc taccgggctt ttgcgccatt aactctttga atttatccgc
    14821 cacgctgagc ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt accagtagct
    14881 gtagcccggc tgattgatct cttttagccg cttcagccgt gtgcgataca acgtggcgcg
    14941 ctcaaatgcc gcatccagca aggcggaacg ccccagtgac agtttcgcca cgtcataaca
    15001 aagttccagg gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat tagctaacag
    15061 ctcacgcagg ttaccgcgat aacctggttc accgagttgc agacgaaaat cctgcgcact
    15121 ctgggcaaga cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt cggtgcggta
    15181 ggcgatggtg atgtcttttg ccaggtcgag cagttgtcga ctggagagtg actgaccaaa
    15241 atactggctg gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc
    15301 cgggatcaac tcgccaaatc cactctcttt aaccaccata tccgccagaa agagatgatg
    15361 gtttaccacc accacatcgg cgtccatcgc ttttttacgt gctttgacca caaagcaatc
    15421 tttatacatc gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga ccagcggcca
    15481 cgcctgcgaa tcttccgcca cgctgacgca ggtgctgata tcaccatcga ctgtttgatt
    15541 agaccaggag cgcagcagga tcacatcgct taatatttgt accggaagat cgccccccgc
    15601 cagcgcctgc tgttcgagac gttcgaggca gaggtagttt gagcgccctt tcagcaacgc
    15661 cacgttgccc gtgtatttca acgcctttga gaccgtcggc aaatcgcggc tatagagctg
    15721 atcctgcaac gcttttgagc cggtcgagat aatgactttc tttttcgccc gcagcgcagg
    15781 ggccaggtaa gcgtaggttt tgcccgtacc ggttcctgct tccaccacca gcggctggcc
    15841 tttttctatc gcctgggtga cggctaccgc catctgtcgc tgtggttctc gcggcttaaa
    15901 gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc
    15961 tgttgatttg aacagggatt atgtcaggat gagggcgctt tcgccagttg aagtggtgac
    16021 ggcgacctca cattgtggca gtctttgcag cacgaaaatg gaaggataat gaggataaaa
    16081 tgatgactat cgttcgtatc gatgctgaag cccgctggtc tgatgtagta atccacaaca
    16141 acacgctcta ctacactggt gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg
    16201 ccaacaccct ggcgcagatt gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca
    16261 ttctggatgc caccattttc ctggccgata aaaacgactt cgcggcgatg aataaagcgt
    16321 gggatgcctg ggttgtcgcg ggtcatgcgc cggtgcgctg cacggtacaa gcgggtttga
    16381 tgaacccgaa gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc
    16441 aaaataagtc agacgataat ttatcgataa tactggtcgg ttatacataa atcga
//
LOCUS       DBFIFS010000092        16490 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.092, whole genome shotgun sequence.
ACCESSION   DBFIFS010000092 DBFIFS010000000
VERSION     DBFIFS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.092"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(29..181)
                     /locus_tag="N7A07_002630"
     CDS             complement(29..181)
                     /locus_tag="N7A07_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2477268.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(445..1179)
                     /gene="cysH"
                     /locus_tag="N7A07_002631"
     CDS             complement(445..1179)
                     /gene="cysH"
                     /locus_tag="N7A07_002631"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2477269.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1253..2965)
                     /gene="cysI"
                     /locus_tag="N7A07_002632"
     CDS             complement(1253..2965)
                     /gene="cysI"
                     /locus_tag="N7A07_002632"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU2477270.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLNQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2965..4764)
                     /gene="cysJ"
                     /locus_tag="N7A07_002633"
     CDS             complement(2965..4764)
                     /gene="cysJ"
                     /locus_tag="N7A07_002633"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU2477271.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5083..5445
                     /gene="queD"
                     /locus_tag="N7A07_002634"
     CDS             5083..5445
                     /gene="queD"
                     /locus_tag="N7A07_002634"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU2477272.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5523..6794
                     /gene="ygcN"
                     /locus_tag="N7A07_002635"
     CDS             5523..6794
                     /gene="ygcN"
                     /locus_tag="N7A07_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2477273.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6785..7045
                     /gene="ygcO"
                     /locus_tag="N7A07_002636"
     CDS             6785..7045
                     /gene="ygcO"
                     /locus_tag="N7A07_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU2477274.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7062..7637
                     /gene="ygcP"
                     /locus_tag="N7A07_002637"
     CDS             7062..7637
                     /gene="ygcP"
                     /locus_tag="N7A07_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU2477275.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7785..8645)
                     /gene="ygcQ"
                     /locus_tag="N7A07_002638"
                     /pseudo
     CDS             complement(7785..8645)
                     /gene="ygcQ"
                     /locus_tag="N7A07_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8642..9421)
                     /gene="ygcR"
                     /locus_tag="N7A07_002639"
     CDS             complement(8642..9421)
                     /gene="ygcR"
                     /locus_tag="N7A07_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU2477276.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9399..10735)
                     /gene="ygcS"
                     /locus_tag="N7A07_002640"
                     /pseudo
     CDS             complement(9399..10735)
                     /gene="ygcS"
                     /locus_tag="N7A07_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10829..12283)
                     /gene="ygcU"
                     /locus_tag="N7A07_002641"
     CDS             complement(10829..12283)
                     /gene="ygcU"
                     /locus_tag="N7A07_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU2477277.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12353..13138)
                     /gene="ygcW"
                     /locus_tag="N7A07_002642"
     CDS             complement(12353..13138)
                     /gene="ygcW"
                     /locus_tag="N7A07_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2477278.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13457..14734
                     /gene="yqcE"
                     /locus_tag="N7A07_002643"
     CDS             13457..14734
                     /gene="yqcE"
                     /locus_tag="N7A07_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU2477279.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14761..16239
                     /gene="ygcE"
                     /locus_tag="N7A07_002644"
     CDS             14761..16239
                     /gene="ygcE"
                     /locus_tag="N7A07_002644"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU2477280.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3892 a         4359 c         4165 g         4074 t
ORIGIN      
        1 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
       61 taaactccat accacgctct ctgatactca actcacataa cgagtcgcct accatgagcg
      121 tgaatcccag tactgtacaa cacagtacta tgattgccac aagggcatat tttgttagca
      181 tgtcttgcct ccctgcaaag aagagactaa catccgaatt gctacgttcg aacgcgagcc
      241 tcaggttgat agaaatatcg cctggggctt ttgtccatct ggaacctcgc gaatgcttaa
      301 cgccagacag cctcaagcac ccgacgccat tctatacctg ataattcttc cagcggcgtt
      361 cacgtttaaa atcaggaatg ctgctcgcaa aataaaaacg cccccatcat tactgacaga
      421 ggcgtttaat ttatccggca atatttaccc ttcgtgtaac ccacattccc tttttaagcc
      481 aaagaaacgc gtttcttctt ccgccatgcc gggttcccat ttacgggttg tatgggtatc
      541 gcccaccgat aaatatcctt cgtcccataa tgggtgatat ttcaggccat tcttttgcag
      601 atactgatat atcgttcggt tatcccagtc gataatcggc agcactttaa atacgccacg
      661 ctgaattgcc agcaccggta aattggcgcg actgccggat tgttcacggc gcaggccagc
      721 aaaccaggtt tgcgcgttaa gttctttcag cgcccggttc atcggttcga ctttgttgat
      781 gtcattgtac ttttcaatgc cttcaacgcc ctgttcccac agttttccgt agcgtgcttc
      841 ctgccaggct gcgctttcgg tagcacggta cactttcagg ttgagcttga gtttgtccgt
      901 taactcgtca ataaagcggt aggtttccgg gaacaagtaa cccgtatcgg tgaggatcac
      961 cggaatatcc gggcgaattt gattcaccag atgcaggctc accgccgcct gaatgccaaa
     1021 gctggaagaa agtacatatt caccgggcag attatccagc gcccaggcta cgcggccttc
     1081 agcgtccagt ttttccagtt gggcgttagt ttccgccagc gccagaatgc gatctacctt
     1141 cggcagttcg ttcagggcgt ttagatcgag tttggacata gattcctcac tgtttgcctt
     1201 gcctgatgcg acgctcacgc gtcttatcag gcctacaaga ccgggctgat ggttaatccc
     1261 acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc gtaaagtcgc
     1321 cgaagccttc acccgcttcg cgctctttcg cccagcgccc tatcagttca tcaagcgacg
     1381 ccaggatttc cggctcggtg atgttttctt tatacatccg tgggatacgt gtcccaatgc
     1441 ggttgccgcc aagatgcagg ttgtagcgac ccggcgcttt acccaccagg cccacttccg
     1501 ccagcatcgc gcgaccacaa ccgttcgggc agcctgttac acgcatcacg atatgctcat
     1561 cgctgacacc atgtttcgcc attaaattat cgatgttgtc gataaaagac ggcaggaaac
     1621 gctccgcttc cgccatcgcc agcgggcaag tcgggaacga gacgcaggcc attgagtttt
     1681 cacgctgcgg cgtgacggca ttcattaacc cgctctcttt ggcgatcttc tcgatcttcg
     1741 ctttctcgct ttccggcaca ccggcgatga tcagattctg gttcgccgta atgcggaaat
     1801 cacctttgtg gatcttcgcg atctccagca ggccggtttt cagcggacgc cccggataat
     1861 caaggatgcg accattttcg ataaacagcg tcaggtgcca gttatcatca atgcccttaa
     1921 cccagccaat acgatctccg cgtccggtga actcatacgg acggatcggt tcaaatttga
     1981 tccccgcgcg acgctccact tccgctttaa acgtctcaac ccccacgcgc tccagcgtgt
     2041 atttggtttt ggcattttta cgatcggttc ggttacccca gtcacgctga gttgtcacga
     2101 cggcttccgc cactgccagc gtatgctcca gcggcagata gccaaactca ctcgccgtgc
     2161 gggcgtaggt tttcttgttg ccgtgttcga tagaaagtcc accgcccacc aacaggttaa
     2221 agcccaccag cttgccgttt tcggcgattg ccacgaagtt catgtcgttg gcgtgcagat
     2281 cgatatcgtt ctgtggcggg atcactaccg tggttttgaa tttacgcggc aggtaggtct
     2341 ggccgaggat cggttcttca tcggtggtgg cgactttttc ctgattgagc cagatctccg
     2401 catacgcgcg ggtacgaggc agcagatgtt cagagatttt cttcgcccac tcgtacgctt
     2461 ctgcgtgcag ctgcgactcg taagggttcg aggtgcagag tacgttacgg ttcatgtcgt
     2521 tagcggtcgc cagcgcatca agaccgaccg agtgcagcat ctggtgcacc ggtttgacgt
     2581 tctttttcag aatgccgtgg aactggaatg tctggcggtt ggtcaggcga atgctgccat
     2641 agatggtgtt ttcaccggca aatttatcga tcgcctgcca ctgtttggtg gtgataactc
     2701 cacccggcag acggcagcgc aacagcatcg catggcgcgg ctccagcttc tgttcagcac
     2761 gttcggcgcg gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg atcagcagga
     2821 agttatcgcc cttaaagccg ccggtcaggc cgtcgtttaa atcttccgca atggtgccgc
     2881 gcaggtagtt gctttcaagc ttcatgcgct cggcgtctgt cagttttcct tcgaccacta
     2941 aaggccctgg atgtttttcg ctcattagta gacatctcgc tgataacggc gctctacgcg
     3001 cagctcactt aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt cggcaatcac
     3061 ttccagaagt gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc agacataaat
     3121 gtgggcaccg tcattgatcc agcgccacag ctccgcgccc tgttcgcgca gtttgtcttg
     3181 tacgtaaact ttttcttttt gatcgcgcga ccaggcaaga tcgatacgcg tcagcacgcc
     3241 ctctttgacg taacgctgcc actccacctg gtagaggaaa tcttccgtaa agtgcgggtt
     3301 gccaaagaac agccagtttt tacccggcgc ttcgtcagcg gcgcgctgct gcataaaggc
     3361 gcggaacggc gcgatgccgg tgcctgggcc aatcataatc accggggttt ccgggttagc
     3421 gggcaggcgg aagttatcgt tatgttcgat aaatacgcga acttcgccct cttcttccac
     3481 gcggtccgcg aggaagctgg aggcaccacc ggcacgggcg cggccttcca cgtcgtaacg
     3541 caccacacca acggtgacgt gtacttcgtt ctcgacttcc tcctgcgagg aggcgatgga
     3601 atacagacgc ggcgtcagcg ggcgcagcag attaatcagc gcttcggcgt ccagttgcgc
     3661 cggagagaag cgcaccatgt cgacaatcgg cgtagtcgcg gcgtaatgct gtaacttcgc
     3721 tttatcgccc accagcggca acagcgtttc gctgcgggta agcgtggcat agttctcaac
     3781 aatattggcg gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt tcagaggcaa
     3841 cgttttgccc tcgacggtga caggttcatc gcctttcagc cacagcagtt cgacaagttc
     3901 tttcaccagt gccggatcgt tctgatacca gacacccagc gcgtcacccg gctggtaacg
     3961 caggcccgag tcacctaagt caatttcgat atggcgaacg tctttttcag agttacgccc
     4021 ggtaattttc tggttaaccg aaaggctcgc taccagcggc gcgtctttgc tgtacgggct
     4081 ggtgtggatt tcatttacca cgccagtagc gacggattgc gaaggtgccg cgacaggcgc
     4141 acgcgattta agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag cctggtattc
     4201 aacgtcggcg tcgacacggt cgagcaggcg ttcaccgccc agttccgcca gcttgctgtc
     4261 gaaatctttc ccggactggc agaaaaattc ataagaacta tcgccgaggc taaacacggc
     4321 aaaagcggta ttttccagct tcggcgcttt tttggagaac aggaacttat gcagcgcgac
     4381 ggcttcttcc ggcggttccc cttctccttg cgttgacgtc accacgataa gcagtttttc
     4441 gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt tcagttttgc
     4501 cgtcaataaa tcatcgcgta atgcttcagc aacccggcgc gcattgccgg tttgcgaagc
     4561 agagataata gttatacccg gcatttctgc ggctggcgct ggcgtcgctg caagcgcagc
     4621 aggctgctga ttgagcacgc cccagaaata gccagaaacc caggcaagct gggtgggagt
     4681 taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg gaagcaacgc
     4741 ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag ctgtttctgc
     4801 gccctgtcag cgcccataaa acagaagaga aggtaaggtt aacggggtga acggtgtgga
     4861 ttaaagacgg gatagcgata actaataacc aaatcgacta acctgtttta gcaatagtct
     4921 ttaacaacaa aatagattaa ccaacctaat gaaaaacaaa tgaatttagc caatcattaa
     4981 gataaatcag cgattttgcg caacaaagtc gttttagata atgcgaaaaa agagcctttc
     5041 cggtactcta cggcggtttt atcgtcctgt agagaaatta tgatgtccac cacgttattt
     5101 aaagatttca ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg gcataaatgt
     5161 ggtcgcctgc acgggcattc ctttatggtg cgactggaaa ttaccgggga agtcgatccg
     5221 catacgggct ggattatcga tttcgctgaa ctaaaagcgg cgtttaagcc aacttatgag
     5281 cgcctcgatc accattatct caatgatatt ccaggtctgg aaaacccaac tagcgaagtt
     5341 ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg
     5401 gtaaaagaaa cctgcaccgc tggttgtatc tatcgcggcg aatgataaga gtgtggcggc
     5461 ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg ttcaggatat ctggggcatg
     5521 acatggaaga cgactgcgac attattatta ttggtgccgg tattgcaggc accgcttgcg
     5581 cgttacgctg cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct gaaatccccg
     5641 gcagcaaaaa tctttccggc gggcggttat atacccatgc actcgcggaa ctcctccctc
     5701 aatttcatct gaccgcgcct cttgaacgac gcatcactca cgaaagcctt tccctgttaa
     5761 cgccggattg cgcaacgacg ttttccagct tacagcccgg cggtgaatcc tggagtgtat
     5821 tacgtgcacg gttcgatccg tggctggttg ccgaagccga aaaagaaggt gtcgaatgca
     5881 tccctggtgc gacggtggat gcactgtatg aagaaaacgg cagggtgtgt ggtgtcattt
     5941 gtggtgacga tattctccgc gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc
     6001 tggctgaacg tcacgggtta gtgactcgtc ctgctggcga agcgatggcg ttggggatca
     6061 aagaagtgct gtcgctggaa acatccgcta ttgaagaacg ttttcatctg gagaataacg
     6121 aaggcgcagc gttgctgttc agcggcggaa tctgtgatga cttacccggc ggcgcatttc
     6181 tttatactaa tcaacaaacg ctctcgttag ggattgtttg cccactctct tcccttacgc
     6241 aaagtcgtgt tccggcaagc gagctgcttg ctcgctttaa aacgcatccg gcagtgcgcc
     6301 cgcttatcaa aaacacggaa tcactggagt atggtgcgca tctggtgcca gaaggtggct
     6361 tgcacagtat gccggtacaa tacgccggta acggctggct gctggtgggc gatgcgttgc
     6421 gcagttgcgt caataccgga atttctgtgc gcggcatgga tacggcgcta actggcgcgc
     6481 aggcggcggc acaaacactg ataagcgcct gccagcaccg cgagccgcaa aatctgtttc
     6541 cgctttatca tcacaacgta gagcgcagcc tgctgtggga tgttctacag cgttatcagc
     6601 atgttccggt gcttttgcaa cgcccgggat ggtaccgtac gtggcctgcg ttaatgcagg
     6661 atatttcccg cgatttatgg gatcagggtg ataaacctgt tccaccgctg cgccagttac
     6721 tctggcgtca tttacgtcgt catggcttgt ggaatctggc gggcgatgtt atcaggagtg
     6781 ttcgatgtct gtagcccgta atctctggcg cgctgctgat gcgccgcaca ttgttccggc
     6841 tgactccgtt gagcgtcaga tggtacaacg gttgattaac gcctgtccgg taggtctttt
     6901 ttcgctcaca ccggaaggtg acttacgcgt tgattatcac ggctgcctgg agtgtggcac
     6961 ctgccgtttg ctgtgcgacg aatccacact acaacagtgg cgctatccgc cttccggatt
     7021 cggcatcacc taccgatttg gataaataat aaggataatt tatgcccctc ttacacctgc
     7081 tccgccagaa tccggtgatt gctgccgtta aagataatgc cagcctgcaa ctggccatcg
     7141 attctgaatg ccagtttatt tccgtcttgt acggcaatat ctgcaccatt agtcaaatcg
     7201 ttaaaaagat taagaacgct gggaaatatg cttttattca tgttgatttg ctggaaggcg
     7261 cgtcaaataa agaagtggtg attcagtttt tgaaactggt gaccgaggcg gatggaatta
     7321 tcagcaccaa agcatcgatg ctaaaagcgg caagagcgga aggttttttc tgtattcatc
     7381 gcctgtttat tgttgattcg atttcgtttc acaacattga taagcaagtt gcgcaatcga
     7441 atccggattg tattgagatc ctgccaggct gcatgcccaa agtgctgggc tgggtgacgg
     7501 aaaaaatccg ccaaccgctg attgctggcg ggctggtatg cgatgaagaa gatgcgcgta
     7561 atgcgattaa cgcgggtgtc gtggcgcttt ccaccacgaa taccggagtc tggacgttag
     7621 cgaaaaaatt gctttgacgg gataatcgta accaattgaa tttggtttga tttgtaggcc
     7681 gcacgccaca tccgacattc agcgcctgat gcgacgctta acacgtctta tcaggcctac
     7741 aggtccccag tccagaaccg taggcaggat catccggcag cacgttattg gcattctgcg
     7801 tcaatgtttg tcaccagtgc ctccagcatc actttccagt catcaaccac gccaacatct
     7861 gcctgtgaaa ataccgctgc gctggcgtca tgattaatcg ccaccacaaa cttgctattg
     7921 cgtacgcccg ccattaacgc tgctgcgcca gaagcgccaa caacaataca cacctcaggt
     7981 gccagcagat gtccggaaat accaatcact ttttcagcat caacgccacc gttcatcact
     8041 cgcgcccgac tgtagcccac ttcggctccc agcttttatg ccagcatagc gatttcctgg
     8101 ttatctgcct ctccgccctg ccccaccacc aacacccgtc tggcctcagc taatgggtcg
     8161 cgggtaacat ttttcaggtt ctctatgcta accagccagt ccggaagcgc acctgggact
     8221 atgttcagtt gctgcatacc agagggcaac gtggcatttt tatctgctcc cgcctggcgc
     8281 gccagggaaa gacatagcgg gcgtttttct gtttgcagcg tcgccgttag cgcatttccc
     8341 cagtgggatt tgcgcacgct tacgattgga atctctaacg aagtcacctg gcagatgctt
     8401 gcaccatgta aacgccaggc cagccgagtc gacagttcat cgccaaacgt tcccggcgga
     8461 aagagaacaa cgttcgcagg cgttcgctgc cactgttcca ccagcgcatc cagcacctgt
     8521 tccaccacta cgggctgtgg ttctatttgc cagtgcgcca gggtacaacc gctgaaatcc
     8581 tgcgccgcca gccagcccgc taatgcggca ttctcctgat ttattgtcac aattgcgata
     8641 ttcatggctg catcctttgg cgtaaatagt cctgccatag cttttgcgct ttttctgcta
     8701 ccgtttgccc atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc agttgcagac
     8761 actgcatcgc tggcaattcg gcagcaacag ttttacggat aatttccgct ttccccgcag
     8821 ccattcgctg gcgcattccc ggtactggca aggccacttc accgcactga cgcacggcaa
     8881 tcaccgcagg caagcgaatc cgacaacagc gcagcccatg ttcagtacgc tgttcgaggg
     8941 tgataaacgg cgcgtcgaga gtgaaacgct ccacctgggt gaagcaaggc cagcccagca
     9001 tttccgccag caaaaatggc gtttgcccat tctgcccttc gctgctttgg cagccggtaa
     9061 ttatcagatc cagcggattc tgacgctgcc attcggcaat atggcgagcg acaaattccg
     9121 acgcaaagcg cagatctgcc gccgtctcca gcaatacagc ttcctcaaac ccaagagcca
     9181 tgagatagcg cagccagtgc aacgcccgtt catcccccat gctcaacgcg gttaaagaca
     9241 tcggcgtacc gttttttctc tgcgccaaca gcagcgcggc ggcagcctgt tcatcagcac
     9301 cgagtaaact tcgcagtagc gaaatatccg gtccgctatt accatgagcc gccgcctgcc
     9361 actctttttc cgccagcatt ccggcatccg gttcggcttt aaacgctaac agaatgttca
     9421 ttcgcacctc ctacatttcc cgccgccacc agcgggagtg ctttggtttc tggtgcccat
     9481 agccaggtta caacaaaacc agccaacaac actgccgcca ggagcaataa ggtgacttgc
     9541 attccccact gcgccagcac ccacggcagc aggccagtac ttaccgccgc ccccaaccga
     9601 ctcatggcgg tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa actttccgca
     9661 ggcaaaatgc ccaccagatt actgactgcc gaaatggtgg tgctgaagag aacaaaagca
     9721 gcagcgttaa tgaactgccg gaaggcaaac aggccatcac taccagcgtt gccgccagca
     9781 gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca
     9841 gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca
     9901 gtccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga
     9961 ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtagcgcgaa gagaacaagg
    10021 ttttgatgtg tttatgggtc gccgttgcca cttcatcgcc cagtaaaaca tggggaccaa
    10081 aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg
    10141 gcgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg
    10201 ccagcagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg gcaatacttg
    10261 ccaacacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaaca ccgcgatggc
    10321 ggcgcgggga aaattcagcc agcaaggtgt gacctactga gtaatcgcct cccagaccaa
    10381 tgccgatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgcaaga
    10441 acgaagcgag cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt
    10501 cggagatcca cccaagaacc aggctgccaa gaaacaaacc aaggtgcgcc gagccgccga
    10561 tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagttgaata atggcgtaac
    10621 caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaattc
    10681 gacagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta
    10741 ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc
    10801 ccatcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat
    10861 tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc cacgcgctgc
    10921 cgtgttccag cttgctccag tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg
    10981 aaccaccgag gcgaatggtt tcttcgcaaa tgattttgtt gagcggattg tggtacttgt
    11041 cgatttcctc ttccggctta cagttgacaa cgttgtagtc gtagacgaag tacatgttgg
    11101 tgccgttctg atagctatga gaggagtgac cgcccagcat ggtgatgtcg tcggcgtgcg
    11161 ggaactcggt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg cagctccagc
    11221 agccggacac ttcggtagta aagcccatgt tgccggtttt gaggatctgc acacgttcgg
    11281 cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc agcttgctgt
    11341 ccacgcgttg gcattgcggg tagcgggcaa cgatttccgc aatcccttcg cccgtcgcct
    11401 tcgcaatgcg agggttacct tcagccataa agatcagcac gcattttccg tcggcaaaat
    11461 gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac ggacgatacc
    11521 cttccaccat gatttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc
    11581 cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact tcagtgatat
    11641 agcacaatgc accttcgttg ccgatgatga tgtgacgaat gtccgggcca gccgcgcggc
    11701 gtggcacgtt tttaatgcgt gtgacggtgc catctgccaa cactgcctcc agaccaacga
    11761 ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg gttgctacca
    11821 ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt
    11881 cacgcaacgc gttttccagc acctccagcg gaacaccaca ttgcgccgtc gcctgcatat
    11941 tctcaatatc aatattaatg atttgattca tggcggaacc gtcgagcacc accgagtttt
    12001 ctacaacagt ttccagccca ccttcggtgg cggaagcacc ggtacgcggc acaccgttaa
    12061 ttttgtgcgc attcataaaa ttcagcacac gggatacttg ctctgtggaa ccgagtttta
    12121 cgaccgctgc cggaatcggc aaagtataaa tgccatgaat atccggaaat ttacgaaaac
    12181 ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa
    12241 tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat
    12301 aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg ggttagcgca
    12361 ctaaataacc gccatcgacc actaataaat gcccgttgac ataattcgat gcctgacttg
    12421 cgagaaacac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat
    12481 gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta atgtctgttg
    12541 cataatagcc aggggcgata ccatttacct gaatattata ttgacctagt tcatcacaat
    12601 aagctttggt gaatccggca agagcatgtt tagtagcaga atatgcaggt gaccattgtc
    12661 cacctaagta agagaacaat gaacagatat taatgatttt accgcttttc tgcgggatca
    12721 taatttttgc cgcttcgtag cttaactcga atgcggcggt caggttcaca tcaatcatcg
    12781 ggtcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt
    12841 taaccagaat atcaactgta ccgaaacgct cacagcaggc agcgataatc ttctgcggcg
    12901 cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctga ttttcaatca
    12961 tttcctttgt ttcgccgtta tctttgacaa aacttggaat aaagacattt gcgcctgctt
    13021 tggccaacgc catggcaaat gcctggccta aaccgctatt cccgccggta acaattgcgg
    13081 ttttaccttt cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag
    13141 ttagctccaa tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag
    13201 ttacctttct cattatctct ggtaactgga gataattttt agcataagga cataactgct
    13261 aatgtgatcg taatcacagt gtgatattcg tattatcaaa tagttttaaa aagcagaagc
    13321 gtgattactc tcacacaaca cctctgcttt ccatgttatt tattatttca attactagag
    13381 acctgagaaa agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta
    13441 caaatgggaa tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat
    13501 tagttttagc ggcggcgtta gtttcgacct ggcttattta cgttatattt atcaaattcc
    13561 catggcgaaa tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg
    13621 tattgcggct attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg
    13681 caaaatgatt acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta
    13741 tccaccgctg tgggtaatgc tctgtattca ggtcgccttt gcgataacaa cgattttaat
    13801 gctgtggtcg gtgtcgatta aagccgcatc gttgcttggc gatcatagcg agcaagggaa
    13861 aattatgggc tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cagtgtttac
    13921 catgtgggtt ttttctcgct ttgcaccgga tgacagcacc agcctgaaaa cggtcattat
    13981 catctatagt gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa
    14041 caacaacctg cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct
    14101 tgccgttttg cgtatcagca ccacctggta ttgcagcatg gtgatttttg gcgtcttcac
    14161 catctacgcc attctgagtt actccaccaa ctatctgacc gaaatgtatg gcatgtcgct
    14221 ggtggcggcg agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc
    14281 acttggcggc attatcacca cctacagtaa agtgaaatcc cctacccgcg tgatccaaat
    14341 cctttccgta ctcggcctgc tggcgttaac tgccctgctc gtcacgaact ctaacccgca
    14401 atcggtcgcg atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg
    14461 cgggctgtac tgggcctgcc ctggcgaagc gagaacaccg tcttacatta tgggcaccac
    14521 ggtaggtatt tgctcggtga ttggattcct gccggatgtc ttcgtttacc caattatcgg
    14581 ccactggcaa gacacccagc ccgcagcaga agcctaccgc aatatgtggc tgatgggcat
    14641 ggcggcgctt gccatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc
    14701 tgatagcgcc cccgcaatgg ccagcagcaa gtaagccagg aaaattacag gagaaaaatc
    14761 atgtcgaaga aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg
    14821 tacgatctgg aaggtaacgt ggtttgcgaa ggcaaaggtt tattgcagcc gatgcacacg
    14881 ccagatgccg ataccgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc
    14941 ggtcacgatt tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg
    15001 ggatccatcc gttgctgccg tgcgttattg aaagccgatg gcacgcctgc tgcgccgttg
    15061 attagctggc aggatgcacg cgtaacgcgt ccctacgaac acactaatcc tgacgtggcc
    15121 tatgtcacct ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat
    15181 atcgccaact attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat
    15241 gctgcggtaa tggagaagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcca
    15301 ggcactgttc ttggacagat cacaccacaa gccgcgctgg cgacacattt cccggcaggg
    15361 ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggagc cggattactg
    15421 gatgatgaaa cggcggtgat ttcactcggt acttatatcg cgctgatgat gaacggcaaa
    15481 gccttgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg
    15541 ctgtatgaag gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg
    15601 ttaggcgagt cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc
    15661 aacaaaaaag cttcttgtgt gccacctggc tgtaatgggc tgatgacggt gctggactgg
    15721 ctgaccaatc cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg
    15781 gattacgcat ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat
    15841 tacgacaata tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggtggc
    15901 ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga
    15961 cgtaacgcca ttaacggttg tgcaagtctg ggggcagcga tcaatacggc ggtaggtctg
    16021 gggctatacc cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa agatatcttt
    16081 ataccgattg agagcaatgc caaacgctac gacgcgatga ataaaggtat tttcaaagcc
    16141 ctaaccaaac atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg
    16201 gggaatgcgg attcgatcca gagctggtcg aatgcgtaag tgaggaagac cgggcgggaa
    16261 actgcccggc ctgaacatac ctgaatggtt atccccgctg gcgcggggaa cataagttcg
    16321 acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga acataataaa
    16381 aactttaact agttcggttt atccctgttt ttacaaggaa tattaactac actcgatgtc
    16441 tttagaataa aagacaatac gaatcgcact ttatcaattt tcacttaaag
//
LOCUS       DBFIFS010000093        16463 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.093, whole genome shotgun sequence.
ACCESSION   DBFIFS010000093 DBFIFS010000000
VERSION     DBFIFS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.093"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            385..537
                     /gene="mokC"
                     /locus_tag="N7A07_002645"
     CDS             385..537
                     /gene="mokC"
                     /locus_tag="N7A07_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU2477281.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(641..1771)
                     /gene="dnaJ"
                     /locus_tag="N7A07_002646"
     CDS             complement(641..1771)
                     /gene="dnaJ"
                     /locus_tag="N7A07_002646"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU2477282.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     TKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1860..3776)
                     /gene="dnaK"
                     /locus_tag="N7A07_002647"
     CDS             complement(1860..3776)
                     /gene="dnaK"
                     /locus_tag="N7A07_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU2477283.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4153..4557
                     /gene="yaaI"
                     /locus_tag="N7A07_002648"
     CDS             4153..4557
                     /gene="yaaI"
                     /locus_tag="N7A07_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU2477284.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4583..5296
                     /gene="msyB"
                     /locus_tag="N7A07_002649"
     CDS             4583..5296
                     /gene="msyB"
                     /locus_tag="N7A07_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU2477285.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5445..6011
                     /gene="satP"
                     /locus_tag="N7A07_002650"
     CDS             5445..6011
                     /gene="satP"
                     /locus_tag="N7A07_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU2477286.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6046..6633)
                     /gene="mog"
                     /locus_tag="N7A07_002651"
     CDS             complement(6046..6633)
                     /gene="mog"
                     /locus_tag="N7A07_002651"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU2477287.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6748..7701)
                     /gene="tal"
                     /locus_tag="N7A07_002652"
     CDS             complement(6748..7701)
                     /gene="tal"
                     /locus_tag="N7A07_002652"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU2477288.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7980..9410
                     /gene="yaaJ"
                     /locus_tag="N7A07_002653"
     CDS             7980..9410
                     /gene="yaaJ"
                     /locus_tag="N7A07_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU2477289.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVYTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9480..10256
                     /gene="yaaA"
                     /locus_tag="N7A07_002654"
     CDS             9480..10256
                     /gene="yaaA"
                     /locus_tag="N7A07_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU2477290.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENTRGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10409..10705)
                     /gene="yaaX"
                     /locus_tag="N7A07_002655"
     CDS             complement(10409..10705)
                     /gene="yaaX"
                     /locus_tag="N7A07_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU2477291.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10919..12205)
                     /gene="thrC"
                     /locus_tag="N7A07_002656"
     CDS             complement(10919..12205)
                     /gene="thrC"
                     /locus_tag="N7A07_002656"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU2477292.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12206..13138)
                     /gene="thrB"
                     /locus_tag="N7A07_002657"
     CDS             complement(12206..13138)
                     /gene="thrB"
                     /locus_tag="N7A07_002657"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU2477293.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13140..15602)
                     /gene="thrA"
                     /locus_tag="N7A07_002658"
     CDS             complement(13140..15602)
                     /gene="thrA"
                     /locus_tag="N7A07_002658"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU2477294.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15628..15741)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15683..15766)
                     /locus_tag="N7A07_002659"
     CDS             complement(15683..15766)
                     /locus_tag="N7A07_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU2477295.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(15980..>16463)
                     /locus_tag="N7A07_002660"
     CDS             complement(15980..>16463)
                     /locus_tag="N7A07_002660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU2477296.1"
                     /translation="HDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVF
                     GREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQQPTKSDTTAD
                     QHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
                     "
BASE COUNT         3837 a         4345 c         4091 g         4190 t
ORIGIN      
        1 aggatttctg agggattaat taggaaaagt aataattaat ttgttgtcag tcggcgatgt
       61 ttgttaggcc aatatttcaa aaataatcca gcttgtcgtc aataattgcg atgaagagat
      121 aaattaaagt taaataatta aatcattaaa aaattacgcc ataaaaatgc gaggcgtctt
      181 ccggattaga atcgataaaa cagaatgggt taaagagagc gatataatag cggcgggtgc
      241 ttgaggctgt ctgtctcagg cattagctga acggcagata gagaaaagcc ccgagtgata
      301 ttttaccatc aacccgaggc cccctatatg ctgaacacat gtagagtgcc tcttactgac
      361 cgtaaggtca aggagaagag agcaatgaag cagcataagg tgatgattgt cgccctgatc
      421 gtcatctgta tcaccgccgt agtggcggcg ctggtaacga gaaaagacct ctgtgaggtt
      481 cacatccgaa ctggccagac ggaggttgct gttttcacgg cttacgaatc cgagtaagag
      541 caacggcggg gagtgatccc cgccacttta caggtgctcg catatcttca acgcacccta
      601 tttttaccca ggcctgccca cgggcaggct tttggggagg ttagcgggtc aggtcgtcaa
      661 aaaacttctt cacaccatca aagaagctct ttgagcgcgg gctgttgtgc tcgccggttg
      721 ggccaccgaa gctttcttgc agctcttgca gcagctgttt ctgcttctcg ttcaggccta
      781 ccggtgtttc gacgacaacg cggcacagca aatcaccctg tgcgccaccg cggacagact
      841 tgacgccttt accgcgcata cggaatagct taccggtctg ggtttcgcca ggcactttca
      901 gtttgacgcg accatcaagg gtcggtactt cgatttcacc acccagcgcc gccatagcga
      961 agttgatcgg gacttcgcaa tacaggttgt tgccttcacg ctcgaaaatc gggtgctgtt
     1021 taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc gtgttcaccc gcttcacctt
     1081 cgcccgcaag acggatgcgg tctccagtgt ccacccctgc cgggatttta acggacagcg
     1141 ttttgctgcg ctcaacacga ccatgaccat gacatttgtt gcacggatct ttgatcagcg
     1201 taccgcggcc ctgacagtgt ggacaggtct gctgcacggc aaagaaaccc tggcgcatct
     1261 gcacctgacc agaaccatga caggtcggac aggtctgcgg ctgtgtacct ggttttgcac
     1321 cgctaccgtg gcaaacgtca cactcttcca gagtcggaat gcggatctct ttggtcacgc
     1381 cacgtacagc ttcttcgagg gtgagctcca tgttatagcg taaatcagca ccgcgcgccg
     1441 cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa aacgtcacca aaaatatcgc
     1501 tgaagtctgc gccgccgcca aaaccgccgc cgcccatgcc accttgctca aacgcagcat
     1561 gaccatactg atcgtatgcc gcacgttttt gcgagtcggt cagaacttca taagcttcct
     1621 tgatctcttt aaatttcgtc tcggcctctt tgtcaccctg gttacggtcc gggtggtatt
     1681 tcatggccag gcgtttgtag gcctttttga tttcacgctc ttccgctgtt ttggaaacgc
     1741 ctaaaatctc gtaataatct tgcttagcca tcttttttaa attgccccta gatgaatgca
     1801 cgggcggaga ggaaattccc cttcgcccgt gtcagtataa taacccgttt atagggcgat
     1861 tattttttgt ctttgacttc ttcaaattca gcgtcgacaa catcgtcatc tttcgcgttg
     1921 tttgcagaag catcagcacc ggcagtctgc tgctgggcat gttgctgctg ggcgatttcc
     1981 atcagtttct gggaaacctg tgccagttcc tgcattttcg cttcgatagc ggctttgtct
     2041 tcacctttca gagcagtttc cagtgcagtc agcgcagact cgatagcagt tttgtcgtca
     2101 gccggcagtt tgtcgcctgc ttcttcaacc tgcttacggg tgctgtgcag cagatggtcg
     2161 ccctggttgc gagtctgtac cagctcttca aacttacggt cagcttcggc gttagcttcg
     2221 gcgtcgcgta ccattttctg gatttcatct tcgttcagac cagaagacgc cttgatggtg
     2281 atcttctgct ctttaccgct gtttttatct ttcgcggaaa cgtgcaggat accgtcagca
     2341 tcgatatcga aggtaacttc gatctgcggc atgccgcgcg gtgccgggtt gataccatcc
     2401 aggttgaact gacccagaga tttgttgtca gccgcacgtt tacgttcacc ctgcagcaca
     2461 tggatggtta ccgcagactg gttgtcttca gcggtagaga acacctggct gtgcttggtc
     2521 gggatagtgg tgtttttcgc gatcagcgtc gtcatcacac cgcccatggt ttcgataccc
     2581 agagacagcg gggtaacatc cagcagcagt acgtctttta cgtcaccagt cagaacacca
     2641 ccctgaacag cagcaccgat tgctacagct tcgtccgggt taacgtcttt acgcggctct
     2701 ttaccaaaga actcagcaac tttcttctga accattggca tacgagtctg accaccaacg
     2761 aggataacgt cgtcgatatc agatacggac aggccagcgt cctgcagcgc aactttcagc
     2821 ggctcgatgg aacggttaac cagatcttca accaggcttt ccagtttcgc acgagtcact
     2881 ttgatgttca tgtgtttcgg accggtcgcg tctgcagtga tgtacggcag gttaacgtcg
     2941 gtctgctgag cggaagacag ttcgattttc gctttttctg ccgcttcttt caggcgctgc
     3001 attgccagcg gatcgttgcg caggtcaatg ccctgatctt tcttgaattc ttcaaccaga
     3061 tagttgatca gacggctgtc gaagtcttca ccacccaggt gggtatcacc gttggttgcc
     3121 agaacttcga aggttttttc gccgtcaact tcgtcgattt cgataataga aatatcgaaa
     3181 gtaccaccac ccaagtcata aaccgcgata gtacggttgc cagtaccttt gtccagaccg
     3241 taagccagcg cagctgcggt cggttcgttg atgatacgtt ttacttccag accagcgata
     3301 cggcctgcgt ctttggttgc ctgacgctga gcatcgttaa agtatgccgg tacggtgata
     3361 acagcttcag ttaccggttc acccaggtaa tcttcagcgg ttttcttcat ttttttcagc
     3421 acttcagcag aaatctgcgg cggtgccatt ttctggcctt taacttcgac ccatgcgtcg
     3481 ccgttatcag cagcaataat tttgaacggc atgatggaaa catcacgctg tacttcttcg
     3541 tcctggaagc ggcggccaat caggcgttta atcgcaaaca gggtgttttg cgggttcgtc
     3601 actgcctgac gtttagccgg ctgaccaacc agagtttcac catcctgggt ataggcaatg
     3661 atagaaggcg tggtgcgatc gccttcggcg ttctccagca cacgaggagt ggtgccatcc
     3721 ataatcgcta cacaagagtt ggtagtaccc aggtcgatac caattatttt acccatctaa
     3781 acgtctccac tatatattcg gtcatcatgt ggttgtgagt ctgtaatagg ggcgaaacgt
     3841 ctggtttcaa ctgcccaatt tcattttttt gcagactcac tcactgcggt tgactactaa
     3901 atggggtcgt aacccacgtc atcaaggggg agatgcaaaa attttttgtg cttttgcgca
     3961 gaaatttcgc gatatggcag gacaagacgt aaaattgaca gcaattttat tcatataaag
     4021 aatgaatcgc agtcaaggta ttcatcaaaa aattgtaata aaaagaaaag attacgtgcc
     4081 tgaatcttct ctttatcagc agtaaactag tgggtattca tccccctacc tcttcccact
     4141 aagagaatcc ttatgaaatc cgtttttacg atttccgcca gcctggcgat tagcctgatg
     4201 ctgtgctgca cggcgcaggc aaacgaccat aaactcctcg gcgtcattgc aatgccgcgt
     4261 aacgaaacca acgctctggc gctgaaactt cctgtttgtc gcattgtgaa acgcatacaa
     4321 ctctctgccg accatggcga tttacagtta agcggtgcat cggtttattt caaagccgcc
     4381 cgtagcgcca gtcagagcct gaatattcct tcagaaataa aagaagggca aaccactgac
     4441 tggatcaaca ttaacagcga taacgacaat aaacgctgcg tctcaaaaat caccttttcc
     4501 ggtcatacgg tgaactcatc ggatatggcc acgctgaaaa ttatcggcga cgactaacgc
     4561 cagaatatag aagccacaaa aaatgaatgt taattacctg aatgattcag atctggattt
     4621 tctccagcat tgtagtgagg aacagttagc aaatttcgcc cggctgctca cccataatga
     4681 aaaaggcaaa actcgcctct ccagcgtact gatgcgcaac gaactgttta aatcgatgga
     4741 aggacatccc gagcaacatc gccgcaactg gcagctgatt gctggagaat tacagcattt
     4801 tggtggcgat agtatcgcca acaaactgcg cggacacggt aaattgtatc gggccatttt
     4861 gctcgatgtt tcaaaacgat tgaagctgaa agccgacaaa gagatgtcta cgtttgaaat
     4921 tgagcaacag ttactggaac aatttctgcg taatacctgg aagaaaatgg acgaggaaca
     4981 taagcaggag tttctgcacg cggtcgatgc cagggtgaat gagctggaag agctgctgcc
     5041 gctgctgatg aaagacaaat tattggcaaa aggcgtgtcg catctgcttt ccagccaact
     5101 gacccgcatt ttacgcaccc acgcagcaat gagcgtactt gggcatggtt tgctgcgcgg
     5161 cgcggggctg ggaggccctg taggcgcggc actaaatggg gttaaagcgg tcagcggcag
     5221 ctcctatcgc gtgacgattc cagccgtact gcaaatcgcc tgcctgcgcc ggatggttag
     5281 cgccactcag gtctgatacc ggaaaccggg gaaaattttc attttcccct caaaagatcg
     5341 tagacactgc cccactggct gattattatg ccgcgccctg aaaacactac agttattcag
     5401 ggaaattatt tcaccattca ttcgatgatg atttttgagg aattatgggc aacactaagt
     5461 tggctaatcc ggcaccgctg ggcctgatgg gcttcggcat gaccaccatt ctgcttaacc
     5521 tgcacaacgt aggttatttc gctctggacg gtattattct tgccatgggc attttctacg
     5581 gcggcatcgc gcaaattttt gctggtctgc tggagtacaa aaaaggcaac actttcggtt
     5641 taaccgcatt cacctcttac ggttctttct ggctgacgct ggttgcgatt ctgctgatgc
     5701 cgaaactggg tctgaccgat gcgccaaatg cacagttcct tggtgtctac ctgggtctgt
     5761 ggggcgtatt tacgctgttt atgttcttcg gcacgctgaa aggcgcacgc gttctgcaat
     5821 tcgttttctt tagcctgacc gtgctgtttg ccctgctggc gatcggtaac attgccggta
     5881 acgccgcaat catccacttt gccggctgga ttggtctgat ctgcggtgcc agcgcaatct
     5941 atctggcgat gggtgaagta ctgaacgagc agtttggtcg caccgttctg ccgattggtg
     6001 aatcccacta attgtcttga gatccccccg ctcgggggga tttttttatt cgctaacgtc
     6061 gcgtcttgca ctcttcggtc tgaatgcagc aaccacttct ggtgccgttt caacgtatgg
     6121 cccttccagc aactgaatgc agtacggtac gctggcaaaa ataccgtgta ccacaacgtt
     6181 accctcagcg tccttcacac cttccagcgt ctctttaata gacttcggct gaccgggtaa
     6241 gttaaggatc agcgcctgtt tgcgaatcac acccacctga cgcgaaagga tcgcagttgg
     6301 tacaaaatgc aggctgatct ggcgcatctg ttcaccaaag ccaggcatct cgcggtccgc
     6361 tactgccagc gtcgcatcgg gcgttacgtc acgacgcgcc gggccagttc cgcccgtggt
     6421 gagcaccaga tggcaactca tttcatccac cagctcacac aacgtttgct cgatgatcgc
     6481 ctgctcatcg gggattaagc gggtttccag ttcaaacggc gtggttagcg ccgatgtcag
     6541 ccattcttcc agcgcaggga tacctttatc ctgataaacg ccgctggatg cgcgatcaga
     6601 gatggaaact aagccaatac gtaaagtatt catcaagatt tttccgttta aacagtctcg
     6661 ttaaacagaa tgatacactg cgaagggagt gacagacagg cttcagaaga ggtagcgtga
     6721 ccgacttccc ggtcacgcta agaatgatta cagcagatcg ccgatcattt tttccagttt
     6781 ttcctggtca atagcaaact tacggatacc ttccgccagt ttatctactg ccattggatc
     6841 ctggttgtgc tgccacagga actcggactc agtgatacgc gccggacgcg ctttcacttc
     6901 gccggtgtaa gacagtttac gttcgatagc cccttcgctc tcagccagct ctttcagcag
     6961 tgccggtgcg atggtcagac ggtcgcagcc tgccagttcc agaatttcgc cgatgttacg
     7021 gaagcttgcg cccataacca cggtttcata accgtgctct ttgtagtact ggtagatttc
     7081 agatacagaa accacgcccg gatcttctgc cggagcgtac tctttcttat cggtattcgc
     7141 tttgtaccag tcaagaatac ggccaacaaa cggcgagatc aggaacacgc ccgcttccgc
     7201 acaagcacga gcctgagcga aggagaacag cagggtcagg ttacagttga taccttcttt
     7261 ttccagctgt tccgcagcac ggataccctg ccaggtagaa gccagtttga tcagaatacg
     7321 atcgttgcta atacctgcat cgttgtagag tttgatcagg cgttttgctt tcgcaattga
     7381 cgcttctgtg tcataggaaa gacgcgcatc aacttcagtt gagatacggc ccggaaccag
     7441 tttcaggatt tccagaccaa tatttactgc cagtttgtca gtcgcgtcca cgatctgctg
     7501 cgcgcgatcg ttgctctgct gtttcgccca ggcgacagca tcatcaatca acttacggta
     7561 ttccggaatc tgcgctgcgt taagaatgag agaagggttg gttgtggcat cctgcggttg
     7621 atacagcttc attgccgcga tgtccccagt gtcggccact acggtggtgt actgacgaag
     7681 ggaggtcaat ttgtccgtca tgatagtatt tctctttaaa cagcttgtta gggggatgta
     7741 accggtctgc cctgatgata tcacgacgca acgtcagcgc aaccgccgac aagacggtta
     7801 tcgccaggcg agactgtttc ggatttctga cactgcgttg atatcgcccg ccatttttaa
     7861 acaaaacctc atgtatgcta cgcagaagtt atcaagtacc tcgtagcgta tatacttctt
     7921 aaacaattgg taacgtttac acaggaaagt catcgcgacc ggcaataaga gggatatgca
     7981 tgccagattt tttctccttt attaacagcg tcctttgggg atcggtaatg atttacctgc
     8041 tcttcggcgc aggttgttgg ttcacttttc gcaccggatt tgtgcagttt cgctacatcc
     8101 gccagtttgg caaaagtctt aaaaatagca ttcatccaca gccaggcggt ttaacctcat
     8161 ttcagtcatt gtgtaccagt cttgcggcgc gcgtgggtag cggcaacctg gccggcgttg
     8221 cgctggctat taccgccggt ggacctggag ccgtcttttg gatgtgggtt gccgcgttta
     8281 tcggcatggc gacctcgttt gccgaatgtt cccttgcaca actttataaa gaacgtgacg
     8341 tcaatgggca gtttcgtggc ggaccggcat ggtatatggc gcgcgggctg gggatgcgct
     8401 ggatgggcgt tctgttcgcc gtctttttgc tcatcgccta cggcataatt ttcagcggag
     8461 ttcaggcaaa cgctgttgcc cgagctctga gtttttcttt tgattttccc ccgctggtga
     8521 caggcattat tcttgctgtc tttgctctgc tggcaatcat tcgcggtctt catggcgtcg
     8581 cccggctcat gcaggggttt gtcccgttga tggcgataat ctgggtactg accagcctgg
     8641 tgatttgcgt aataaatatc gggcaacttc cccacgtcat ttggtctatt tttgagagtg
     8701 cttttggctg gcaggaagcg gcaggcggcg cggcgggata taccttaagc caggcgatta
     8761 ctaacggttt tcagcgcagt atgttttcca atgaggcggg aatggggtcg acgccaaacg
     8821 cggcagcggc agcggcgtcc tggcctccgc atccggcagc gcaaggaatt gtccagatga
     8881 ttggcatttt tatcgacacc ctggtcatct gtacggcaag cgccatgctg atattactgg
     8941 cgggtaacgg cacaacctac atgccgctgg aaggtattca gcttatccag aaggcgatgc
     9001 gggtgctaat gggttcctgg ggtgctgaat ttgttaccct cgtggttatt ctgtttgcct
     9061 tcagctccat cgttgccaac tacatttatg ccgaaaacaa tctcttcttt ttacgcctga
     9121 acaaccctaa agcgatctgg tgtttgcgga tctgcacctt cgcaacggtc atcggcggca
     9181 ccttgctaag tcttccgctg atgtggcaac tggcagatat cataatggcc tgcatggcta
     9241 ttaccaattt gactgccata ttactgctct cgcctgtggt ttataccatt gccagtgatt
     9301 atctacgcca gcgtaaactc ggcgtgcgcc cggtgtttga tccgttgcgt tatccggata
     9361 tcggtcgcca gctttctcgg gacgcgtggg atgatgtttc gcaggagtaa tcacaactat
     9421 cgatcaactc attctctttt tttgctaaag tcggcataaa tttcctgcaa ggactggata
     9481 tgctgattct tatttcacct gcgaaaacgc ttgattacca aagcccgttg accaccacgc
     9541 gatatacact gccggagctg ttagacaatt cccagcagtt gatccatgaa gcgcggaaac
     9601 tgacgcctcc gcagattagc acgctgatgc gtatcagcga caaactggca ggtatcaacg
     9661 ccgctcgctt tcatgactgg cagccagatt tcacgccgga gaatgcccgc caggcgattc
     9721 tggcgtttaa aggtgatgtc tacaccggct tgcaggccga aaccttcagc gaagacgatt
     9781 tcgattttgc ccaacagcat ttgcgaatgc tttccggctt gtatggcgta ctccgcccgc
     9841 tcgatttaat gcagccttat cgtctggaaa tggggatccg tcttgagaat acccgaggga
     9901 aagatctgta tcaattctgg ggagatatca tcaccaacaa gctgaacgag gcgctcgcag
     9961 cacaaggcga taatgtggtg attaatctgg cgtcagatga gtattttaaa tcggtgaagc
    10021 cgaagaaatt gaatgccgag attatcaagc cggtgttcct cgatgagaag aacggcaaat
    10081 ttaagataat cagcttctac gctaagaaag cacgcggtct gatgagtcgt ttcattattg
    10141 aaaatcggct gaccaaacca gagcaactga ctggttttaa tagcgaaggt tacttctttg
    10201 atgaagcttc ctccagcaat ggcgaactgg tgtttaaacg ctacgagcag cgttaagttt
    10261 gtagtcaatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca tgcagattca
    10321 atatattgca gaattcacgt aggcctgata agacgcgcca gcgtcgcatc aggcgctgaa
    10381 tgccggattg ttacccggca tttgtcattt agcggtgatg tttgcctgga ccatgaccgc
    10441 cgtgatgatc atgatgagct ttcttatggt gacgcggcgg tggcggcggt ccgtgtgggt
    10501 gccagcgatt gcctcgccat tcataatgtt gtttccacca gccgtggtcg cgccagtgac
    10561 cgccatccca gtaataaccg cgattatcac gatcgcctat ctgtaatttt actgacggga
    10621 ctaacgtaat ttccgcagcc tgcgttgcca tgggagcgac cagaaccagg gaaagtgcga
    10681 gtacgataga ttgcatcttt ttcacaggtt actcctttta tcattgcccg ttatgggtcg
    10741 atatccggag attacgcgat gagagcggcg gttgttattc tccgcaatcc taatctctaa
    10801 gttaccgcat ttattgagaa tttctccttt ttccctgtcg tttctctcca tattttcttc
    10861 acaaaaaagc cgggctgcat aaaagcaaac ccggcctgat tgagataatg aatagatgtt
    10921 actgatgatt catcatcaat ttacgcaacg cagcaaaatc ggcgggcaga ttatgcgaaa
    10981 gcaagggtaa atcggcacgt tctgccagct cttttggcag atccaacgtt tcaccgagaa
    11041 tcgcttccac gctctcttta aatttcgccg gatgcgcggt gccgaggaac aagccatatt
    11101 cgcctggatt caactggtca cgcagcgcac gatacgctac ggcagcgtgc ggctccgaag
    11161 tgtagcccag ttcttttaac tcacgcattg tctgttgcgt ggtttcatca tccacggcgg
    11221 cataacccag ctctttcagt tgccagattt tgcggcggaa caactcttcc acacgcggcc
    11281 agttgttcgg ctgactcacg tccatcgcgt tggataacgt cgcctgagtc gctttgggtg
    11341 accactgacc gtcatgcagg aaacgtggca cggtatcgtt cacgttggtc gcagcaataa
    11401 aacgtttcac cggcagaccg agtgacttcg ccagcagacc cgccgtcaaa tcgccgaagt
    11461 ttccgcttgg caccgagaca accagctggt tgcgcgcttc ctgcggcagc tgcgcaactg
    11521 cttcaaagta gtagcaaatc tgcgccagca accggctaat gttaatcgag ttagctgagt
    11581 ttaaccccag cgccactttc agctcttcat catcaaacgc ctgcttcacc agcgcctgac
    11641 aggcatcgaa atcgccgtcg atggcaacag tttcgatatt gccgcccaat gtacagaaca
    11701 gtttttcttg cagtggactg attttgcctc gtggataaag gataaccact ttcacattcg
    11761 gtaaaccgta gaaagcatga gccactgccg ctccggtatc accggaggtc gcggtcagaa
    11821 tggtcactgg cttatcgccc gcaatatggg tcagcatttg tgccataaag cgaccgccga
    11881 aatctttaaa tgccagcgtt ggcccgtgga acaattccag acaaccgaca tcgctttcaa
    11941 cattggcgac cggagccggg aaggcaaacg ccgcgcgcac gcgctcttcc aggatttcct
    12001 gcgggatttc atcaccaata aacgccgaga ggatcttcgc actgcgggtg acaaaatcca
    12061 gcttcagcat ctcatcaatt tcagtcaggc tgaattccgg caggtcgtgc ggaaaaaaca
    12121 gcccctgatt tttgcccaac ccctgggtta cggcttgcgc aaagctgacc tgctcattgt
    12181 gatctttaag attgtagagt ttcatttagt tttccagtac tcgtgcgccc gccgtatcca
    12241 gccggcaaat atgaacaaaa ccttcctgat tttgcaggta gttcttaccc aaccagtcgg
    12301 caacgcgctg ggcggtatcc ggcttgtcac acagagcaaa caaggtcggg ccggagccgg
    12361 agataccgct cgctaccgcg ccgatttccg cgaccgcctg ccgcgcctgc cggaagccag
    12421 gcagtaaccg ttcacggtag ggttctgcga taacatcttt catcagcttc gcggcaagct
    12481 caggctgacg ggaatagcag gcgtgaatga agcctgccaa gtgtcgcccg tgcgcaatgc
    12541 aatcctggcg gcgatactgc gccggtaaaa tagcccgggc ttctgccgtc gagactttaa
    12601 tccccggata cgccagcacc cacagccact catcaaaccc tggcacttgc tggctgatga
    12661 tgtcgttttc ttcgatcatc aactgcatac caccaagaaa acacggtgcc acgttgtcgt
    12721 aatgaatgct gccggagatt cgtccttcca gctcgcccat caaagccagc aaacgggtgt
    12781 cattaagcgg cttgccgcag tgttcattca tcgccatcag agccgcgacc accgaacagg
    12841 cgctggagcc taagcccgaa ccgatcggca tattcttttc cagagtcatc gccactggaa
    12901 tttgcttgcc cagctcctgg caaaaacgct cccagcactg ataaacgata ttttcccgtg
    12961 gttctgacgg cagcttatcg gcaaagcgtc cgaggttgtt gagactgaat gtctctgccg
    13021 actcaaccgt gactacatct ccgagcaatg caccatcaac gggtgtcacc gccgccccga
    13081 gcacatcaaa cccgacgctc atattggcac tggaagccgg ggcataaact ttaaccatgt
    13141 cagactccta acttccatga gagggtacgt agcagatcgg caaagacacc ggcagcggta
    13201 acgtcattgc ccgcaccata tccgcgcagc accaacggca gcggctgata atagtggcta
    13261 taaaaggcca gggcgttttc gccatttttc actttgaaca gcggatcatt accatccact
    13321 tcggcaatct tcacgcggca gacgccatct tcatcaatat tgccaacata gcgcaaaact
    13381 tttccttcat cacgggcctt cgccacgcgc gcggcaaaga gatcgtcgag ctgtgacaga
    13441 ttcgccataa aagcggcaac atcaccctca gcgttaaact ctgcgggcag cacaggttca
    13501 atttcaatat ccgccagctc cagttcacgt cccgtttcac gagcgagaat taatagttta
    13561 cgcgctacat ccataccaga aagatcatct cgcggatccg gttcggtata acccatttcc
    13621 cgcgccagcg tagtcgcctc ggagaaactc atgccttcgt ctaacttgcc gaagatataa
    13681 gaaagcgaac ctgaaagaat gccggagaac ttcatcaatt catcaccagc attgagcaga
    13741 ttttgcaggt tctcaataac cggtaatcca gccccaacgt tggtgtcata gaggaattta
    13801 cgccgcgatt tttcagccgc atgacgcaac agatggtagt aatccatcga cgaggtgttg
    13861 gcctttttgt tcggcgtgac aacgtggaaa ccttcgcgca ggaagtcggc atattgatcc
    13921 gccactgcct ggctggaggt gcagtcaaca atgaccgggt tcagcagatg atattctttc
    13981 acgaggcgaa ttaagcgccc gagattaaac ggctctttgg cttgcgccag ttcttcctgc
    14041 cagttttcca gatttaggcc atgcacattg gtgagcagag ccttcgagtt agcaacaccg
    14101 cagacacgta agtcgatatg tttattcttc aaccagcttt gctgacgctt cagttgctcc
    14161 agcagcgcac cgccaacgcc accgacgcca atcacaaaca cttcgataac ctgatcggta
    14221 ttgaacagca tctgatgagt aacgcgcacg ccagtggtcg catcatcgtt atttaccacg
    14281 acagagattg agcgttcaga agatccctga gcaatagcga caatgttgat attggcgcgg
    14341 gccagcgcgg caaagaattt cgccgagatc ccacgcaagg tgcgcatacc atcacctacc
    14401 accgagataa tggccagccg ttccgtcacc gccagcggct ccagtaagcc ttctttcagt
    14461 tccaggtaga actcttcctg cattgcccgt tcagctcgca cacagtcgct ttgcggaacg
    14521 cagaaactga tactgtattc ggaagatgat tgcgtaatca gcaccacgga aatacgggcg
    14581 cgtgacattg cagcaaagac gcgcgccgcc atgccgacca ttcctttcat ccccgggccg
    14641 gaaacgctga acattgccat attattcaga ttggaaatgc ccttgaccgg taattcgtct
    14701 tcatcacgac tggcaccaat gagcgtacct ggagcttgag gatttccggt atttttaatc
    14761 aggcaaggga tctggaactg ggcgatgggg gtaatggtgc gggggtgaag aactttagcg
    14821 ccgaagtagg aaagctccat cgcttcctgg taggacatcg atttcaacaa cctcgcatcg
    14881 ggcacctgac gcgggtcgca ggtatatacc ccgtcaacgt ccgtccaaat ctcgcaacaa
    14941 tcggcgcgta aacaggcagc cagcaccgcc gcggagtagt cggaaccgtt gcgtccaagt
    15001 accaccagtt cgcctttttc attaccggcg gtgaaacctg ccatcagcac catgtgatca
    15061 gccggaatac gacttgccgc aatacggcgg gtggactctg caatatcgac agtagattcg
    15121 aggtaatgcc ccactgccag cagtttttcg accggatcga taacggtaac gttgtgaccg
    15181 cgcgcttcta atacgccggc cataatggcg atcgacattt tctcgccacg gcaaatcagc
    15241 gcagcgttaa tgctatccgg gcactgccct aacaaactaa tgccatgcag gacatgtttt
    15301 atttgggcaa attcctggtc gacgaaagtt ttcaattgcg ccagcgggaa tcccggctgg
    15361 gcggcggcga gtcccgtcag aagttcggca aaaatacgtt cggcatcgct gatattgggt
    15421 aaagcatcct ggccgctaat ggttttttca atcatcgcca ccaggtggtt ggtgattttg
    15481 gcgggggcag agaggacggt ggccacctgc ccctgcctgg cattgctttc cagaatatcg
    15541 gcaacccgca gaaaacgttc tgcatttgcc actgatgtac cgccgaactt caacactcgc
    15601 atggttgtta cctcgttacc tttggtcgaa aaaaaagccc gcactgtcag gtgcgggctt
    15661 ttttctgtgt ttcctgtacg cgtcagcccg caccgttacc tgtggtaatg gtaatggtga
    15721 tggtggtggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat gttgtgtact
    15781 ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt agtgacctaa
    15841 gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc tgtcagagac
    15901 tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa aagctgaaaa
    15961 atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc agcaaacggt
    16021 gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgttgtaac cagtcgacca
    16081 gttttatgtc atctgccact gccagagtcg tcagcaatgc catggctcgt tcgcgtaaag
    16141 cttgcagttg atgttggtct gccgttgtgt cacttttcgt cggttgttgt attaatattg
    16201 ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga taatccgcca
    16261 ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc agcccggaat
    16321 cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc tctaacagcg
    16381 gcaccagttc tactggcgta gcgtagtaat gatatttcgc ccgactgcgc gcagtggtgg
    16441 cgacagtgaa atcgacatcg tgt
//
LOCUS       DBFIFS010000094        16314 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.094, whole genome shotgun sequence.
ACCESSION   DBFIFS010000094 DBFIFS010000000
VERSION     DBFIFS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.094"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..105)
                     /locus_tag="N7A07_002661"
     CDS             complement(<1..105)
                     /locus_tag="N7A07_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477297.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIK"
     gene            complement(252..1535)
                     /gene="yeaH"
                     /locus_tag="N7A07_002662"
     CDS             complement(252..1535)
                     /gene="yeaH"
                     /locus_tag="N7A07_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU2477298.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1648..3582)
                     /gene="yeaG"
                     /locus_tag="N7A07_002663"
     CDS             complement(1648..3582)
                     /gene="yeaG"
                     /locus_tag="N7A07_002663"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU2477299.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4018..4764
                     /gene="mipA"
                     /locus_tag="N7A07_002664"
     CDS             4018..4764
                     /gene="mipA"
                     /locus_tag="N7A07_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU2477300.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4854..5708
                     /gene="yeaE"
                     /locus_tag="N7A07_002665"
     CDS             4854..5708
                     /gene="yeaE"
                     /locus_tag="N7A07_002665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU2477301.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5759..6643)
                     /gene="yeaD"
                     /locus_tag="N7A07_002666"
     CDS             complement(5759..6643)
                     /gene="yeaD"
                     /locus_tag="N7A07_002666"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU2477302.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6727..7722)
                     /gene="gapA"
                     /locus_tag="N7A07_002667"
     CDS             complement(6727..7722)
                     /gene="gapA"
                     /locus_tag="N7A07_002667"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU2477303.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8064..8477
                     /gene="msrB"
                     /locus_tag="N7A07_002668"
     CDS             8064..8477
                     /gene="msrB"
                     /locus_tag="N7A07_002668"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU2477304.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8519..8791
                     /gene="yeaC"
                     /locus_tag="N7A07_002669"
     CDS             8519..8791
                     /gene="yeaC"
                     /locus_tag="N7A07_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU2477305.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9161..10237
                     /gene="ydjL"
                     /locus_tag="N7A07_002670"
     CDS             9161..10237
                     /gene="ydjL"
                     /locus_tag="N7A07_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU2477306.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10264..11643
                     /gene="ydjK"
                     /locus_tag="N7A07_002671"
     CDS             10264..11643
                     /gene="ydjK"
                     /locus_tag="N7A07_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477307.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11660..12703
                     /gene="ydjJ"
                     /locus_tag="N7A07_002672"
     CDS             11660..12703
                     /gene="ydjJ"
                     /locus_tag="N7A07_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGU2477308.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12724..13560
                     /gene="ydjI"
                     /locus_tag="N7A07_002673"
     CDS             12724..13560
                     /gene="ydjI"
                     /locus_tag="N7A07_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGU2477309.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13565..14497
                     /gene="ydjH"
                     /locus_tag="N7A07_002674"
     CDS             13565..14497
                     /gene="ydjH"
                     /locus_tag="N7A07_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU2477310.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14507..15487
                     /gene="ydjG"
                     /locus_tag="N7A07_002675"
     CDS             14507..15487
                     /gene="ydjG"
                     /locus_tag="N7A07_002675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGU2477311.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15624..>16314
                     /gene="ydjF"
                     /locus_tag="N7A07_002676"
     CDS             15624..>16314
                     /gene="ydjF"
                     /locus_tag="N7A07_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2477312.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYII"
BASE COUNT         4239 a         3671 c         4119 g         4285 t
ORIGIN      
        1 ttttattgat ggcagagata tatagttttg accaggatca attttcgtta acgataaaaa
       61 atatttaaag aataacccca gggaaatacg tgttgacaga ataatttttc cgaatgattg
      121 ttttctggaa ctaaattgtg taaaggttat gctgtcatat ttaaagcgat tgtaagctaa
      181 tgtatgtaat aaatgagata acttacagtg agctaaaaat cagccaggcg ataatgaccc
      241 ggctgatagt tttagtcttt agctgttgca ttttgcttat gaaacagttc acggaacacc
      301 ggataaatat catcctggtc gcggatatgc tgcatcgcaa agttgtcgaa agtagattgc
      361 agatgctcat attctcgcca cagtgtctga tgcgcgcgac gggtaatttc gatatagctg
      421 taataacgaa caacgggtaa taattttttc gccagaattt catggcaaag cggagagtca
      481 tcggcccagt tatcgccgtc cgatgcttgt gcagcgtaaa tattccactg tgccgggtta
      541 taacgctctt tcactacctc atccatcagt ttcagggcgc tggaaacaat ggtgccgcca
      601 gtttcctgcg agtagaaaaa ctcatgttca tcgacttctt tcgcctgggt atgatggcgg
      661 atgtatacga cttccacgtt cttatacgtt ctgctgagga acagatacag cagaatataa
      721 aaacgcttag ccatatcttt agtggattga tccattgaac cggaaacgtc catcaggcaa
      781 aacatcactg cctggctgga gggatcgggc cgcttctcgt agttcttgta acgtaaatcg
      841 aaggtgtcaa taaaagggac gcgttcaatt ttggcacgta attctgcaat ttctttgcgc
      901 agacgttcct cttccagcag ctgcgcaggt tcactgttgc tgatgatggc caaattctct
      961 tccagtgcat gaagttcccg ccgcttgcct gccgtcatgg ctgtgcgtcg cgccagtgag
     1021 ttctgcaagg aacgcacaac gctgatattg gccggaacgc cgttagcggt ataacccgcc
     1081 cgatgcgttt tatattcggt cagctggcgt tgttggtttt gtttcagatt cggtaaggcc
     1141 aaatcttcaa agagcagatc aagatactca tctttcgaaa tctgaaagac aaattcatcc
     1201 tgaccttcac catcctggct ggcctggccc tgaccactgc cggaacctcc gccgccaccc
     1261 tggggacgtt caattcggtc gttctgaacg aaatggtcat tgcccggatg cacgcggtgg
     1321 cgcagaccgc cacgcccctg atgaaacatt ggttcgctaa tatcttccgt gggaatggat
     1381 acggactcgc cgctgtcgac gtcagtcacc gaacgcttat taatggcctc ggagatcgac
     1441 tgtttaattt gcgctttata acggcgtaaa aaacgctggc gattcaccat gcttttgttt
     1501 ttgccgttca gacgccggtc aataaaccag gtcatatgcc ccccgtacta catttgccaa
     1561 ctgtatgcta cgcctgtgtt gccatttctc tggcccacgg ttcgctgtcg ttgtaggccc
     1621 ggtagcatgt gtaccgggcc agggttgtta agacgattta cgtacgcgca aataccattc
     1681 gcacagtaaa cgcacctgtt tacgggtgta gcctttttcc atcatacggt cgacaaagtc
     1741 gtcgtgtttc ttctgctcgt cggttgacgt tttggcgtta aacgagataa ccggcaacag
     1801 ctcctcggta ttggagaaca ttttcttctc gataaccgtg cgcagttttt catagctggt
     1861 ccagttcgga ttgcgtccgc tgttattggc tctggcgcgc agtacgaagt taacaatctc
     1921 gttgcggaaa tcttttggat tactgatccc cgccggtttc tcgattttct ccagctcggc
     1981 gttaagagac tcgcggtcaa acagctgccc ggtatccgga tcgcgatact cctgatcctg
     2041 aatccagaaa tccgcgtagg taacataacg gtcgaaaatg ttttgcccat attcggaata
     2101 ggattcaagg taggccgtct ggatctcttt gccaataaac tcggcatatt tcgggatcag
     2161 ataacctttc aggaactcca gatagcgttc tgcctgctct tgtgggaact gctcgcgctc
     2221 gatctgctgt tccaggacgt agaacagatg gaccgggttt gccgccactt ctacatgatc
     2281 gaagttgaac acgcgggaga ggatcttaaa cgcaaaacgc gtcgacagac cgttcatacc
     2341 ttcatcgaca ccggcgtagt cacgatattc ctgatacgac ttggctttgg gatcggtgtc
     2401 tttcagactt tcgccatcat aaacccgcat ctttgaataa atgctggagt tttctggctc
     2461 tttcaggcgc gaaagaatgg aaaaacgtga cagtgtttcg agagtgccag gggcgcatgg
     2521 ggcgtgagtc aattcactgt gattaagcaa tttctcgtag attttgatct cttcggaaat
     2581 gcgcaagcaa tacggcacct tcacgatgta aacacgatcg aggaaggctt cgttgttttt
     2641 gttattacgg aaagtgaccc attcggactc gttcgagtgg gcaagaataa tcccgttgaa
     2701 cggcagggcg gagatacctt ccgtcccgtt gtagttacct tcctgagtgg cggttaacaa
     2761 gggatgcagc acttttatcg gtgctttaaa catctcgaca aattccatga tcccttggtt
     2821 ggcgcggcac agcgcaccgg aatagccgta ggcgtccggg tcattctgcg cgtaatgttc
     2881 gagtttacga atatcgactt tcccaaccag cgcggagatg tcctggttgt tctcatcacc
     2941 gggttccgtt ttggcgatag caatttgttg cagaattgac ggccagacct tcactacccg
     3001 gaacttagtg atatcgccac caaattcatg caggcgtttt gccgcccacg gcgacatgat
     3061 ggtgccgaga taacggcgag gaatgccata ctctttttcc agaatctgcg catcttcctg
     3121 cggattgaaa agacagaacg gatgatcgtt gaccgggcta cgctcaccgt tggcgctcaa
     3181 tacataaatc ggtacgagct gcattaatga tttcagtcgc tcagcaagcg atgatttacc
     3241 cccacccaca ggccccagca gatacaggat ttgtttcttc tcttccagcc cctgagccgc
     3301 gtgtttcaga taagagacaa tctgttcaat cgcgtcttcc atgccgtaaa actcttcaaa
     3361 cgccggataa cgtgcaatga cccggttaga aaagagtcga gaaagtctgg gttcctgggc
     3421 tgtatcgacc atgacaggct caccgatagc catcaatagc cgctcagccg cgttggcata
     3481 agcactgcga tcttgccgac aagtggtaag aaactcctgc agtgtgaact cttcgtcctt
     3541 ggcagcttca tatcgctggc gatagtgatc gaatatattc atggtatgcc gtcctttcgt
     3601 tttttagcac aggttaagag ccgttcatat gaatagtaga ggctcccgga agaggtaaac
     3661 tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc attttatcga cgatgagaac
     3721 aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg gcaaatggaa aacttgccta
     3781 cacggctttc ttaatttcaa tggcatatca acaactcatc cataaatttt gcataattaa
     3841 tgtaaagacc aggctcgcca gtaacgctaa attcatttgg ctgtaagcgc ggtgtcatcc
     3901 gcgtcaggaa aattaaacag ttactttaaa aaatgaaaac gtaaaaaggt tgggtttcga
     3961 tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt ttaaggaatg attaattgtg
     4021 accaaactca aacttctggc acttggagtg cttatcgcaa cgtctgcagg cgtagcgcac
     4081 gctgaaggta aattttccct gggcgcaggc gtaggtgtcg ttgaacaccc atataaagat
     4141 tacgataccg atgtttaccc agtaccggta atcaactatg aaggcgataa cttctggttc
     4201 cgtggcttag gtggtggtta ctacctgtgg aatgacgcaa cggataaact ttcaattacc
     4261 gcttactggt cgccgcttta cttcaaagct aaagacagtg gcgatcacca aatgcgtcac
     4321 ctggatgacc gtaagagcac catgatggct ggtctgtctt atgctcactt tacccagtac
     4381 ggttacctgc gtaccaccct ggctggcgat accctggata acagcaacgg catcgtctgg
     4441 gatatggcct ggttgtatcg ttacaccaac ggtggcctga ccgtgactcc gggtattggt
     4501 gtgcagtgga acagcgaaaa ccagaacgaa tactattatg gcgtatcgcg caaagagtcc
     4561 gctcgcagcg gtctgcgtgg ctataactcg aacgacagct ggagccctta cctggagctg
     4621 agcgccagct acaacttcct cggcgactgg agtgtttacg gtaccgcgcg ctacacccgt
     4681 ctgtctgatg aagttactga cagcccgatt gtggataaat cctggactgg cctgatttct
     4741 accgggatca cctacaaatt ctgataatgc atctttccag ggcattttgt gcataaaaac
     4801 agggcgtcac gccctgtttt gcattacggt ggtgcaatac ctgggaggaa acaatgcaac
     4861 aaaaaatgat tcaatttagt ggcgatgtct cactgccagc catagggcag ggaacatggt
     4921 atatgggcga agatgccagt cagcgcaaaa cagaagttgc tgcactacgc gcgggcattg
     4981 aactcggttt aaccctcatt gataccgccg aaatgtatgc cgatggcggt gccgaaaagg
     5041 tggttgggga agcattaacc ggtctgcgag agaaggtctt tctcgtctct aaagtctatc
     5101 cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga agccagttta cgccgtctca
     5161 atactgatta tctcgatctc tacttattac actggtctgg cagtttcgct tttgaagaga
     5221 ctgttgcagt gatggaaaaa ttgatcgccc agggaaaaat ccgccgctgg ggcgtttcga
     5281 accttgatta tgctgatatg caggaactct ggcagctgcc ggggggaaat cagtgtgcca
     5341 ctaatcaggt gctttaccat ctcggttcac gagggattga gtacgatcta ctcccctggt
     5401 gccagcaaca gcagatgccg gtgatggctt acagtccgtt agcccaggcc gggcggttgc
     5461 gcaatggact gttaaaaaac gcggtagtca acgaaattgc acatgctcac aatatcagcg
     5521 cggcgcaagt attgttggcg tgggtaatca gccatcaggg tgtgatggcg attccaaaag
     5581 cggccacgat tgcccatgtc caacagaatg cggctgtgct tgaggtcgaa ctttcgtcag
     5641 cggaattagc catgctggac aaagcgtatc cggcaccaaa aggaaaaact gcgctggata
     5701 tggtgtgatg ttgatacgtg ccggatgcat cgcacccggc acacaacatt gacgcaaatt
     5761 aacgtttggc aacgcgaatg gattgcgcca gatgtgcagg tttttctttg gtcacttttt
     5821 gcatttctga ggcgtaagct gtttctacac aaacaaaggt cttgtagcca tcgtccggca
     5881 tgtcgcccat gctaactgaa agcgccggtc ccgggttcca gcctacaacg ttcagatgat
     5941 gctggtggcc tacggcgata atacgattca gcgcttcatc attaatcacg ctgcaatctt
     6001 gcggattcag atacacgcga tcggtacggt cagggaaggt ctgaataccg tcggtcagta
     6061 catcttcttt cgcgtcattt actttatcaa tgaagcgatc gcccagccca ctgacgctta
     6121 ccttagcgat atcacccacg ttaaagtagg tatgcagggc agaggtggtt tcaaattcgc
     6181 cgtgtgcttc aagatcgatt tcacagtttt tatccacgcg gaaatgcgct aacagagtaa
     6241 agtcgtgcag ccagaatttt ttcgtctctt cgctttgtgt caattcaaaa gtcagcgcta
     6301 cgccattagc atcttcacga tgtgatttca gcgtccacgg caggttgcgg gcaaaaccgt
     6361 gcgcaggcag gccttgttgt gccgccggac caaaccacgg ccagcaaacc ggtacgccac
     6421 cgcgaatagc aacgccattt ttgaacggtg tgttgttgct caaccacaga acttcttctt
     6481 cacctgcagg tttccacgag agaaggtgtg cgccctgtaa tgcaaaagag gcttttacct
     6541 ggggatgatc gactacaatg aggtccagtt catccagttt acgacgggag aggacagggg
     6601 agatttgttc gatgaccgga agggcaaaaa ttttcttaat catgacgcag tcctttaact
     6661 tcattttatc aggtaaaaaa aagagcgacc gaagtcgctc tttttagatc acagtgtcat
     6721 ctcaacttat ttagagatgt gagcgatcag gtccagaact ttgttggagt aaccggtttc
     6781 gttgtcgtac caggatacca gtttcacgaa gttgtcgttc agagcgatac cagctttagc
     6841 atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta gatactacgt catcttcggt
     6901 gtagcccaga acgcctttca tttcgccttc agcagcagct ttaacggcag ctttgatctg
     6961 ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca actacggata cgttcggggt
     7021 cggaacgcgg aacgccatac cagtcagttt gccattcagt tctggcagta ctttacctac
     7081 agctttagca gcaccggtag aggacgggat gatgttctgg gaagcgccgc ggccgccgcg
     7141 ccagtctttg tgagacgggc catcaacggt tttctgagta gcggtagtag cgtgaacggt
     7201 ggtcatcaga ccttcgatga tgccgaagtt atcgttgata actttagcca gcggagccag
     7261 gcagttggtg gtgcaggaag cgttggaaac gatgtcctgg ccagcatatt tgtcgaagtt
     7321 agcgccttta acgaacatcg gagtgttgtc tttagacgga ccagtcataa ccactttctt
     7381 cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc aggaacagac cagttgcttc
     7441 agcgacaacg tcaacaccaa cttcgtccca tttcaggtta gccggatcac gttcagcggt
     7501 aacacggatt tttttaccgt taacgatcag atgaccgtct ttcacttcaa cggtaccgtc
     7561 gaaacggccg tgagtggagt catatttcag catgtatgcc atgtaatcag cgtctaacag
     7621 gtcgttgatt gcaacgatct cgatgtcaga acgtttctga gcagcacgga aaacaatgcg
     7681 accgatacgg ccaaaaccgt tgatacctac tttgatagtc atatattcca ccagctattt
     7741 gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct tacgcagcgt caagcggaat
     7801 cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca ttacgcgact gactcgcctc
     7861 actcttcctt tgggcttgag accacatggg gtcggcgccc cgaattttaa agggcaatta
     7921 cgataaaaat gtgattttca tcacgattta ggtgcagcca tttcgtcgag atcaagttta
     7981 ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa tcagtcacgt aatgtgagca
     8041 cgattaaagt gagatgtgag caaatggcta ataaaccttc ggcagaagaa ctgaaaaaaa
     8101 atttgtccga gatgcagttt tacgtgacgc agaatcatgg gacagagccg ccatttacgg
     8161 gtcgtttact gcataacaag cgtgacggcg tatatcactg tttgatctgc gatgccccgc
     8221 tgtttcattc ccaaaccaag tatgattccg gctgtggctg gcccagtttc tacgaaccgg
     8281 tgagtgaaga atccattcgt tatatcaaag acttgtcaca tggaatgcag cgcatagaaa
     8341 ttcgttgcgg taactgtgat gcccatctgg ggcatgtctt ccctgacggg ccgcagccaa
     8401 cgggcgaacg ttattgtgtt aactctgctt ctttacgctt cactgatggt gaaaacggcg
     8461 aagaaatcaa cggttgaaaa aacgattcag caacttattc cacaggagcg gaaaaattat
     8521 gaatcttgat gacattatca acagcatgac gcctgaggta taccagcgtt tgtcgaccgc
     8581 cgttgaactg gggaaatggc ctgatggcgt tgcgttgacc gaggaacaaa aagagaactg
     8641 cctgcaactg gtgatgctgt ggcaagcccg caataatacc gaagcacagc atatgacaat
     8701 tgacacaaat ggtcagatgg tcatgaagag caaacagcag ttaaaagaag attttggtat
     8761 ctcagcaaag cctattgcca tgtttaagta agtggttcat cccctttttg ccgcggctat
     8821 gaaggggata gccaacaatg accccacgac tcatctcaat tatctcgcca tgacctggct
     8881 aagtcccagt ccgatctctt gccgtcgcgt tcacgcctga atgacttctc ctgaacttaa
     8941 gtctcagacc tatttggccg gtaatccctc tcaaatttgc tccttttgcg ttccgcctta
     9001 catcaacctg taacagaacc agccattttc tgcgtaattg tgatctgagt tgtaaaacgc
     9061 agattattat ctgtgtttta catgtttaat gttgacctca aaaatcatgt gggtaagtat
     9121 gatgaccatc taagcaaaac aaaacacaga ggatgacgca atgaaagcac tggctcggtt
     9181 tggcaaagcc tttggcggct acaagatgat cgatgtgcca caacccattt gtggcccgga
     9241 agatgtagtg attgaaatta aagccgcggc aatctgcggc gcagacatga agcactacaa
     9301 tgtcgatagc ggttctgatg agtttaactc tatccgcggc catgagttcg caggttgtat
     9361 tgcgcaggtt ggtgaaaaag tcaaagactg gaaagtgggg caacgcgtcg tgtcggataa
     9421 cagcggtcac gtctgcggcg tttgtccggc ctgtgaacaa ggtgattttc tgtgttgtac
     9481 agaaaaagta aaccttggtc tggataacaa tacctggggc ggtggttttt ccaaatattg
     9541 tctggttcct ggtgaaattc tcaaaattca tcgtcatgcg ttgtgggaaa tccctgatgg
     9601 tgttgattat gaggacgcag ccgtacttga ccctatctgt aatgcctaca aatccatcgc
     9661 gcagcaatcg aaattcctcc ctggtcagga tgtggtcgtc atcggcactg gcccactcgg
     9721 gctgttctcc gtacaaatgg cgcgaattat gggggcggta aatatcgtcg tcgttggtct
     9781 gcaagaagat gtggcggtcc gcttcccggt tgcaaaagaa ctgggtgcga cggcagtagt
     9841 aaatggttct accgaagatg tggtggcgcg ctgccagcaa atttgtggca aagacaatct
     9901 gggactggtg attgaatgct ccggtgccaa tatcgcactg aaacaagcca tcgaaatgct
     9961 ccgtccgaac ggggaagtgg tacgcgttgg aatgggcttc aaacctcttg atttctcgat
    10021 taatgacatt accgcctgga acaaaagcat cattgggcat atggcctatg actccacctc
    10081 atggcgtaac gctatcaggc tattagccag cggcgctatc aaagtcaaac cgatgatcac
    10141 gcatcgtatc ggcctgtcgc aatggcgcga agggtttgat gcgatggtcg ataaaaccgc
    10201 aatcaaagtg atcatgactt acgactttga tgaataacca ctctcctgaa ggaacgttat
    10261 cgcatggatc agataacaaa accgcattgt ggtgcacggc tggatcgctt accggattgc
    10321 cgctggcatt catcaatgtt tgccatcgtc gcgtttggct tgcttgtctg ctggagtaat
    10381 gccgttggtg gcttgatcct cgcgcagctg aaagcgttgg gctggacaga taattccacc
    10441 acagccacat tctcagcaat cacgaccgcc ggaatgtttc tcggtgcgct ggtgggcggc
    10501 atcattggcg acaaaaccgg tcgcagaaat gcgttcatcc tctatgaggc cattcatatt
    10561 gcctcgatgg ttgtcggtgc tttctcaccg aatatggatt tccttattgc ctgccgtttt
    10621 gtgatgggag ttgggctggg agctttactg gttacgctgt ttgctggttt caccgaatat
    10681 atgcccggta gaaatcgtgg aacgtggtca agtcgggttt cctttattgg caactggtca
    10741 tatccgctct gttcattgat agcgatggga ctcacgccgc tgattagtgc agagtggaac
    10801 tggcgggtac aactgcttat ccctgcaata ttgtcgctta tcgctacggc gctggcctgg
    10861 cgctactttc ctgaatcccc gcgctggctg gaatcgcgcg gacggtatca ggaagccgag
    10921 aaagtcatgc ggagtataga agaaggcgtc atacgccata caggtaaacc tttgccgccc
    10981 gtggttattg ctgatgacgg taaagcacca caagcggtgc catattcagc cttactgaca
    11041 ggagtattac tgaaacgcgt gatattaggc tcttgtgtgc tgattgccat gaacgttgtg
    11101 cagtacacac taattaactg gttgccaacg atattcatga cccaggggat taatttaaaa
    11161 gactcgattg ttttaaatac catgagtatg tttggcacgc catttggcat ttttattgcc
    11221 atgctggtga tggataaaat tccgcgtaaa acaatgggtg tggggctatt aatcctgatt
    11281 gcggtgctcg gatatatcta ttcactgcaa accagcatgt tgctcataac gttgattggt
    11341 ttcttcctga ttactttcgt ctatatgtac gtttgctatg cctcggcagt gtatgtccct
    11401 gagatctggc cgacagaggc caaactccgt ggctccggtc tggcgaatgc ggtagggcga
    11461 atcagtggta ttgccgcacc ttatgccgtt gcagtgctgc tcagtagtta tggcgttacg
    11521 ggagtcttta ttcttctggg ggcggtttca attattgtcg caattgctat cgccaccatt
    11581 ggaatcgaaa ccaaaggtgt ctccgttgaa agtttaagta ttgatgcagt agccaataaa
    11641 taatacgggt ggattcaaaa tgaaaaattc aaaagcaata ttgcaggtgc cgggcacaat
    11701 gaaaattatt tcagcagaaa taccagtgcc taaagaagat gaagttttga ttaaagtaga
    11761 atatgtcggt atttgtggtt cagatgtaca tggttttgaa tcaggtccgt ttattccgcc
    11821 taaagaccca aatcaagaaa ttggcctggg tcatgaatgc gccgggacgg ttgtggctgt
    11881 gggaagccgt gtgcgcaaat ttaaaccggg ggatcgggta aatatcgaac ctggcgttcc
    11941 ttgcggtcac tgtcgttact gtctggaagg caaatataac atctgcccgg acgttgattt
    12001 tatggcgaca caacccaact accgcggcgc attaacgcac tatctgtgtc atccggagag
    12061 ctttacttac aaactgcccg acaatatgga cacgatggaa gggacgctgg tggagcctgc
    12121 cgcagtcggg atgcatgccg cgatgctggc agatgttaaa ccgggtaaga agataattat
    12181 tctgggagca ggttgtattg gtttgatgac gttgcaagcg tgcaaatgcc tgggagcaac
    12241 ggaaattgcc gtcgttgatg tgctggaaaa acgtctggca atggcggaac agcttggtgc
    12301 gacagtggtt attaacggcg caaaagaaga cactattgca cgctgtcagc aatttaccga
    12361 agacatgggc gcagatattg ttttcgaaac agcgggttct gcggtcaccg ttaaacaggc
    12421 accttatctg gtaatgcgtg gcggtaaaat tatgattgtt ggtactgtac ccggcgcttc
    12481 ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc cagacggtat tccgctatgc
    12541 caatcgttat ccggtcacga ttgaagcaat ttcttcaggg cgattcgatg tgaaatcgat
    12601 ggtgacgcat atttacgatt atcgggatgt acaacaggca tttgaagagt cagttaacaa
    12661 caaacgcgac attattaaag gcgttattaa gattagcgat taattaatac gggagaacag
    12721 aacatgctcg cagatatcag atattgggaa aacgatgcca caaataaacg ttatgctatt
    12781 gcccatttta atgtatggaa tgcagaaatg ttgatgggcg ttatcgacgc agccgaagaa
    12841 gcgaaatcac cggttattat ttcttttggc acaggttttg tcggcaacac ctcatttgaa
    12901 gatttctctc acatgatggt atctatggca caaaaagcaa cggtgccggt aataactcat
    12961 tgggatcatg gtcggagtat ggagattatt cataacgcct ggactcatgg tatgaattca
    13021 ttaatgcgtg atgcttccgc atttgatttc gaagaaaata ttcgtttaac caaagaggct
    13081 gttgatttct tccatccgct gggtattccg gtagaggcgg aattagggca tgtcggtaat
    13141 gaaaccgttt atgaggaagc gctggcgggc tatcactata ccgatcctga ccaggcggct
    13201 gaatttgttg aacgtacggg ctgtgactca ttggctgtcg ccatcggcaa ccagcatggg
    13261 gtttatacgt cagagccaca attgaacttt gaggtcgtca aacgcgtacg cgatgcagtg
    13321 tctgttccgc tggttttgca tggcgcatcg gggatcagtg atgccgacat taaaactgca
    13381 atttcgttgg gtatcgcgaa aatcaacatc catacggagc tctgtcaggc cgcaatggtc
    13441 gcagtgaaag aaaaccagga tcagcctttc ctgcatttag aacgtgaagt acgtaaagct
    13501 gtaaaagaac gggcattgga aaaaattaaa ctgttcggtt ctgatggcaa agcggaataa
    13561 tgatatggat aatctcgacg ttatttgtat aggtgccgct attgttgata ttccattgca
    13621 accggtcagt aaaaatatct ttgatgtgga ttcttatcct cttgaaagaa tcgcaatgac
    13681 caccgggggt gatgcaataa atgaagcaac aattatttct cgtctgggcc atcgtacagc
    13741 attaatgagc cgtattggta aagatgccgc agggcagttt attctcgacc attgccgcaa
    13801 agagaatatt gatattcaaa gcctgaagca ggacgtcaac atagatacct ctattaatgt
    13861 tggactggtg acggaggatg gcgagcggac gtttgtcaca aaccgtaacg gcagtctgtg
    13921 gaaattgaat attgacgatg ttgattttgc gcggttttcc caggcgaaat tattatcact
    13981 ggccagtatt ttcaacagtc cactattgga tggtaaagcg ctaacagaaa tttttacgca
    14041 agctaaagcc cggcagatga atatctgtgc cgatatgatt aaaccgcggt tgaatgaaac
    14101 gctggatgat atttgcgaag cattgagtta tgtcaattat gccgaggcaa aattactcac
    14161 cgggaaagag acactggatg aaattgctga cagctttctt gcgtgcggcg taaaaacggt
    14221 ggtgattaaa acgggtaaag acggctgctt tatcaagcgt ggtgacatga cgatgaaggt
    14281 gccggcggtc gcaggaataa ccgccatcga caccattggc gcgggcgata actttgcttc
    14341 aggttttatt gcggcactgt tagaaggaaa aaatctgcgt gaatgcgcac gctttgccaa
    14401 tgcaacggcg gctatctcgg ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa
    14461 gctggtcgaa caattgctgg aagaatacga aggataatga aggcaaatga aaaagatacc
    14521 tttaggcaca acggatatta cgctttcgcg aatggggttg gggacatggg ccattggcgg
    14581 cggtcctgca tggaatggcg atctcgatcg gcaaatatgt attgatacga ttcttgaagc
    14641 ccatcgttgc ggcattaacc tgattgatac tgcaccagga tataactttg gcaatagtga
    14701 agttatcgtc ggtcaggcgt taaaaaaact gccccgtgaa caggttgtag tagaaaccaa
    14761 atgcggcatt gtctgggaac gaaaaggaag tttattcaac aaagttggcg atcggcagtt
    14821 gtataaaaac ctttccccgg aatctatccg cgaagaggta gaagccagct tgcaacgtct
    14881 gggttttgat tacatcgata tctacatgac gcactggcag tcggtgccgc cattttatac
    14941 gccgatcgct gaaactgtcg cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc
    15001 tataggcgct gctaacgtcg atgctgacca tatccgcgag tatctgcaat atggtgaact
    15061 ggatattatt caggcgaaat acagtatcct cgaccgggca atggaaaacg aactgctgcc
    15121 gctatgtcgt gataatggca ttgtggttca ggtttattcc ccgctagagc agggattgtt
    15181 gaccggcacc atcactcgtg attacgttcc gggcggcgct cgggcaaata aagtctggtt
    15241 ccagcgtgaa aacatgctga aagtgattga tatgcttgaa cagtggcagc cactttgtgc
    15301 tcgttatcag tgcacaattc ccactctggc actggcgtgg atattaaaac agagtgattt
    15361 aatctccatt cttagtgggg ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact
    15421 gaatatcaac ttatcggatg cagacgcaac attgatgagg gaaatggcag aggtcttggg
    15481 gcgttaaata ctcaacacga ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg
    15541 attcaaacag gttattttga aagtaaacat cggttatgcg ataatcgcgc taatgtgatg
    15601 taaaaagata caggggtgta atcgtggcgg caaaagacag gattcaggca attaagcaaa
    15661 tggttgccaa cgataaaaag gtgacagtct caaatttgag tgggattttt caggtaaccg
    15721 aagaaaccat tcgccgcgat cttgagaagc tggaagatga aggctttttg accagaacct
    15781 atggtggtgc tgttttaaat acagcgatgt tgacggagaa tatccatttt tataagcgcg
    15841 cttcatcgtt ttatgaagag aagcagctta ttgcacgcaa ggcactaccc tttatcgaca
    15901 ataaaaccac catggcagcc gattccagta gtaccgtaat ggaattgctc aaattgttac
    15961 aggaccgtag tgacctgacg ttgctaacca actcggcgga agcgattcat gttctggctc
    16021 agtcagaaat taaagtcgtt tcaacgggtg gggaactaaa caaaaataca ctttcactgc
    16081 aaggaagaat tactaaagag atcatcagcc gctatcatgt cgatatcatg gtaatgagtt
    16141 gtaaaggtct tgatattaac agcggcgcgc tggactctaa tgaagcggaa gctgaaatca
    16201 aaaagacaat gatccgtcag gcgacagaag ttgcgttatt ggttgatcac tctaagtttg
    16261 atcgcaaagc ttttgtccag ttagccgact ttagtcatat taattacata ataa
//
LOCUS       DBFIFS010000095        15998 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.095, whole genome shotgun sequence.
ACCESSION   DBFIFS010000095 DBFIFS010000000
VERSION     DBFIFS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.095"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(185..1234)
                     /locus_tag="N7A07_002677"
     CDS             complement(185..1234)
                     /locus_tag="N7A07_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU2477313.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1366..2583
                     /locus_tag="N7A07_002678"
     CDS             1366..2583
                     /locus_tag="N7A07_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477314.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(2587..3144)
                     /gene="dcrB"
                     /locus_tag="N7A07_002679"
     CDS             complement(2587..3144)
                     /gene="dcrB"
                     /locus_tag="N7A07_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU2477315.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3217..3882)
                     /locus_tag="N7A07_002680"
     CDS             complement(3217..3882)
                     /locus_tag="N7A07_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU2477316.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            4103..4348
                     /gene="tusA"
                     /locus_tag="N7A07_002681"
     CDS             4103..4348
                     /gene="tusA"
                     /locus_tag="N7A07_002681"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU2477317.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(4450..6648)
                     /gene="zntA"
                     /locus_tag="N7A07_002682"
     CDS             complement(4450..6648)
                     /gene="zntA"
                     /locus_tag="N7A07_002682"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU2477318.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(6722..7348)
                     /locus_tag="N7A07_002683"
     CDS             complement(6722..7348)
                     /locus_tag="N7A07_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU2477319.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            7489..7848
                     /locus_tag="N7A07_002684"
     CDS             7489..7848
                     /locus_tag="N7A07_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU2477320.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(7851..8120)
                     /locus_tag="N7A07_002685"
     CDS             complement(7851..8120)
                     /locus_tag="N7A07_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU2477321.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8110..8706)
                     /gene="rsmD"
                     /locus_tag="N7A07_002686"
     CDS             complement(8110..8706)
                     /gene="rsmD"
                     /locus_tag="N7A07_002686"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU2477322.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            8856..10352
                     /gene="ftsY"
                     /locus_tag="N7A07_002687"
     CDS             8856..10352
                     /gene="ftsY"
                     /locus_tag="N7A07_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU2477323.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10355..11023
                     /gene="ftsE"
                     /locus_tag="N7A07_002688"
     CDS             10355..11023
                     /gene="ftsE"
                     /locus_tag="N7A07_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU2477324.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            11016..12074
                     /gene="ftsX"
                     /locus_tag="N7A07_002689"
     CDS             11016..12074
                     /gene="ftsX"
                     /locus_tag="N7A07_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU2477325.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12319..13173
                     /gene="rpoH"
                     /locus_tag="N7A07_002690"
     CDS             12319..13173
                     /gene="rpoH"
                     /locus_tag="N7A07_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU2477326.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13444..14547
                     /gene="livJ"
                     /locus_tag="N7A07_002691"
     CDS             13444..14547
                     /gene="livJ"
                     /locus_tag="N7A07_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU2477327.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            14697..14963
                     /gene="ataR"
                     /locus_tag="N7A07_002692"
     CDS             14697..14963
                     /gene="ataR"
                     /locus_tag="N7A07_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HGU2477328.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            14970..15497
                     /gene="ataT"
                     /locus_tag="N7A07_002693"
     CDS             14970..15497
                     /gene="ataT"
                     /locus_tag="N7A07_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HGU2477329.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(15494..15877)
                     /gene="panM"
                     /locus_tag="N7A07_002694"
     CDS             complement(15494..15877)
                     /gene="panM"
                     /locus_tag="N7A07_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU2477330.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         3920 a         4091 c         4323 g         3664 t
ORIGIN      
        1 aaaaataaca caatgttatc agtgcattct ttgagaagga tttaatcgct attcggcgtt
       61 tatatatatt tgacttaagg aatggttttt ttaaattaat tttctgaata atgtttcatc
      121 atttcctcaa atcaaatggc cactgttaga taaacggtgg ccattgcctg ctcaggaggt
      181 gctattaacc cggtaaccga cttttcggtc tgcccggccc cagtaaaatc gccagtttgc
      241 tgccgccttt cgtcgtttcc atccagattt tacacacgct ggttaatggc accgaaagta
      301 gcatccctac cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg
      361 tggacatccc caggcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat
      421 ggaccactaa aaacaatgcg ccgaccagaa tacattcgta aacaccatta aacagcagca
      481 cctgaatcat tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttaagca
      541 aaaacgccag tactgcccac atcagcgcaa actgtacccc catcagctcc agccccagcc
      601 agacgatgac acccgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc
      661 ctttaagtgc gcggtgtaat cccgcgatgt gaatctgtgg gttattcagc gcaaaacgca
      721 ttttgtaagg gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa
      781 gcacgctcgc cattgccccg gaaagcccgg tcattagcgc tgtggtgaag gtaaccactt
      841 tttccgagtc catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca
      901 acatctcctg caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag
      961 agataaattc gttaaacgat gccgccagta cgccgaccag cgcggttagt gcgatcagca
     1021 tcaccaccac tacaatcgta atggcaacgg ggcgttgtac tccccgacga ataaaccagg
     1081 tgacgagcgg gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat
     1141 ctgccgctgc gtgaatgccc gcgaggatca ctaccagcga ggccagcttg agcagaatgt
     1201 gcatgcccgt tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtat
     1261 taagtgtagc gggagtcacg acggcaatat tctgattctt actgctgatt ttcacgtcag
     1321 cacgtggtaa aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta
     1381 gccgaacccg cgctaaacgg attgcgcctg aatttgcgca ttctctccat tgtcatgttt
     1441 aacttcgcca gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat
     1501 gatgtgatgg gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc
     1561 accttgctga gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt
     1621 gtcgtcttcg gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta
     1681 accgccagtc tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt catccttggg
     1741 attgggcaaa gttttgccgg aacgggatcg accctgtggg gtgttggcgt ggttggctcg
     1801 ctgcatatcg ggcgggtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg
     1861 ggtgcgccgt taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatt
     1921 attatgggcg tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc
     1981 agtaaaggca aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg
     2041 gcgctggcac tggcttccgc cggatttggc gtcatcgcca cctttatcac gctgttttat
     2101 gacgttaaag gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc
     2161 ggtacgcgtt tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtagcgatg
     2221 atttgcttta gcgttgagat aatcggcctg ctactggttg gcgtggcgac tatgccgtgg
     2281 atggcgaaaa tcggcgtctt actggcgggg gccgggtttt cgctggtgtt cccggcattg
     2341 ggtgtagtgg cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacctac
     2401 accgtattta tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagt
     2461 tgggcgggcg taccggtgat ttatctggcg gcggcgggac tggtcgcaat cgcgttatta
     2521 ctgacgtggc gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc ctcatcatct
     2581 taaaacttac tgaataacca gcgtattaat gatgttttct gcggtggtct gcgctttttg
     2641 ctgatcgtca gctggcagag taatttgcat ggtcagcagt tgattaccca cgttacccag
     2701 aataacggaa gagtacgccg tctggccttt cgcggagata atactgtcta actgctgcat
     2761 tttgtgacct ttcagctcaa tggctttatt ggtgaccact tgcagctgtg gatcgcggct
     2821 acgttgctga tcttccagac gcttcgccag caccgccaga tcttctttcg gatcatcgcc
     2881 catgatgacg atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc
     2941 ctgcgttccc agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt
     3001 gccatcaagc aggttgacgg gattcccggt agcgttactt tccgcgaccg aaccctgcgc
     3061 cgtagcgtta gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc
     3121 gacatattta accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc
     3181 attcatccat cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat
     3241 ttcggattta tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag
     3301 gaagaaaacg atactgatta acactttgaa acagtaatcg accagcgcga tttccatcca
     3361 gtgttcagcc ataaaggcat ccgggctacg ccagaaggca atgaagaaaa aggccagcgt
     3421 gtcgctgacg ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagc ggcgactctg
     3481 acgcaggcgg ttaaaaacgt gcacgtcgag gatctgtccc agcgcgtagg ccatgaaact
     3541 ggcggtggcg atacgggcga caaacaggtt gaagtgggcg agtgcgccga atccctgcca
     3601 tgaacccata tagaatagcg acgagatgac gtaggagatc agcaatgccg ggatcatcac
     3661 cgcaaagatg atacgacggg ccagcggtgc gccaaaaata cgtacggtca ggtcggtagc
     3721 gagaaagata aacggaaagc taaacgcgcc ccaggtggta tggaaaccaa aaatggagac
     3781 gggaagctga accagatagt tactggaggt gatcaccagc agatgaaata acgataacca
     3841 gaacaacgcc ttatagcgtt gagtttgcga gaaaacgttc atattgtacc tttttgatta
     3901 accattgggg tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt
     3961 gcttaccctt ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa
     4021 ttttcacgca atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt
     4081 ttttttatgt gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga
     4141 cgcacttggc ctgcgctgcc cggaaccagt catgatggtg cgcaaaacag tgcgcaatat
     4201 gcagccaggt gaaacattgc tgattattgc tgacgatccg gcgacgaccc gcgatatccc
     4261 tgggttttgt acctttatgg aacacgaact ggttgccaaa gagacggatg gactgcctta
     4321 tcgttatttg attcgtaaag gcggttgacg ggggcagatt ggcttcgatg ccgccttttc
     4381 ccctcaccct aaccctctcc ccagaggagc gaggggaccg atcgcgctca atgttgcgat
     4441 cggtttgcct tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc
     4501 acccgtatct gccagcactg ccagccatag tccggtcatc cctaacagcg tggtgacgag
     4561 gaagatccct ttcagcccca gcgcaatagt gatgttctgg cggatattgg cgtgagtggc
     4621 gcgtgccagt tcaatcattt gcaccagacc gcgcaggtgg ttatgggtta atgctgcgtc
     4681 ggcggtttcc agcgccacgt ctgtgccgct gcccattgca atcccgatgg cggcagcttt
     4741 catcgctggc gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag
     4801 ctcggtcacc gctttgactt tatcttccgg caacaggccc gctttaaact ccagccccag
     4861 ctccccggca attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac
     4921 gcccagcgcg ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgcaa
     4981 tgcaatgata cccagcacgt catcattacg tactaccagc acaaccgttt gcccggcgct
     5041 ttccagttca ttaatcaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcatat
     5101 caacacgcgc tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga
     5161 ttcggcggcg ggaatggcga gtgcagcaac ctgtgcttcg cgcacgatgg cctgcgccag
     5221 tggatgcgtc gcgccttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat
     5281 acccgttgcc ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt
     5341 atcaaacgcc acctgggtaa cacgacccag ctgttccagc gccgctccgc ctttaatcaa
     5401 cgccccacga cgcgctgccg ccgccagccc ggaggtaatc gccgcaggcg ttgagataac
     5461 taacgcacac gggcagccaa tcagcagcag cgtcagccct ttataaatcc actcctgcca
     5521 gctggtggca aacagcagcg gtggcaccag cgtcaccagc agagcgacgg ccataatcgc
     5581 gggcgtgtaa atacggctga aacggtcgat aaaccgctca atgggtgcgc gacgctcttc
     5641 ggcttcttcg atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac
     5701 ttccagcgtt accagacggt ctacgctggt ggctcctgca ggaactttat cgcccgttgc
     5761 gcgctccacc ggaatagatt cgccggtcag ggcgctttca tcaaaactgg caaacggtga
     5821 gagcagttta ccgtcggcag gcaaacgtcc acctgcggcg acttcaatca catcgccagg
     5881 gcgcaggctg ttaatcgcca cctcttcccg ctcaccgtta cgcaggcgcg tggcggtttc
     5941 tggtttcagc gccattaacg cgctgacccc ttgacgcgcg cggctggcgg cccagccttc
     6001 cagtcgttca ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat
     6061 aaacagtgca ccaatagcgg ctacgctcat taaggtttca atggcgaagt agctgccgga
     6121 tttgatcagc cgtaatgcct gacgagcaat cgggtacagc ccaaccagcg tggtcgcgat
     6181 aaacgccagt tgcccgaacg gatgattaaa ttgctccaaa ccccagctga ttgccatcat
     6241 cacgataagc gtaatcagcg gcagattctc tttcaggcgc gatgcttgtg gctcgtcggc
     6301 ggcctgctca tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcacg
     6361 gatgtcattg tcggcatcaa ccaccagttt ttcggtggcg aacaacacct gtacctgatt
     6421 cacgcctgca agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat
     6481 gccgctgact ttccagctat agcgggtgcc ggagacgttt tcagagagag ttggcgtgct
     6541 ggaacatgcg ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa
     6601 cgcagcaaat tgaggggctt tcttgccgtg attgtcagga gtcgacatgg catcctccgg
     6661 ttaagttttt tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt
     6721 atcagagata cagcgagcgg acgatgagga agtgaccggc aaagtagcag gccgcagcaa
     6781 tcgcgttgtc cgcacggaag cgtcggcgat agtgactacc cagccagaca aagttactga
     6841 taaacagcaa cgaagcgccg acaaacgcag agagtgccgg agcggtcgga cggaagaacc
     6901 acagttcacc tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc
     6961 aacgatactc ttccagccgc gtccagataa tcgccagtaa cagcgcaccc agaaccagca
     7021 acaccagtgg cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt
     7081 acagcaggtg cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca
     7141 gggttagcgc atcgcccagc agtgaggcgc acagccctgc cagcaccaga tagctaatgg
     7201 cgtcgaacat cggcgcttgc caggccagta acagcaggag aagaagggtt aacggtttaa
     7261 acacccagcg ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa
     7321 gacagacagc gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa
     7381 gcggaatgcc tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca
     7441 taactggcag ggtatgctta tttccgcatt ttccgatgag ggataaagat gagcaaacca
     7501 cctcttttct ttattgttat cattggctta atcatcgtcg ccgcatcgtt tcgttttatg
     7561 caacagcgac gggaaaaagc tgataatgat atggcaccac tccagcaaaa gctggtgttg
     7621 gtgagcaaca agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact
     7681 ccggcaggca ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag
     7741 cagacgtttc gcctcgacgc ccagcagtac cacgccctga cagtgggcga taaaggtacg
     7801 ctgagctata aaggaacgcg ctttgttagc tttgttggcg aacaataagg ttatttttta
     7861 actttaaatt tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc
     7921 ttttcggcgg tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagt
     7981 tgcataccat gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg
     8041 cgcgggaagg gatgcaccag gttgaggatt aaaaatcccc aaacacagag cattaacaaa
     8101 cgaccaatat taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc
     8161 cacctgaccc gccacttttt cccgatgtaa cgaccagttt gccggaacgg tgggcagacc
     8221 gttttcgact tcgctttcga cataaatcag ggtttcgtca gccagccagc cgttatcttc
     8281 cagtaaattt atcgtctctt ctaacaaacc acggcggaac ggtggatcga caaacacgat
     8341 attatgcggt gtgccttttt gcgccaggaa tgacattgcg ttgctgttca ccacgcgtgc
     8401 attgcctgct tttagtgtcg ccagattctt aattaactgc tgagaaactg cacgatccat
     8461 ctcaattaac gtggcccccg cagcgtagcg cgataacgct tccagcccca gcgcgccgct
     8521 cccggcgaag caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa
     8581 cgtttcgcgt acgcggtcgg tggtggggcg cagacctggg ctatcaggaa ccgggagttt
     8641 acggcctcgc cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt
     8701 tttcatgata aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt
     8761 aagttaagtg atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct
     8821 gtgccatgaa gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct
     8881 ttttttcctg gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc
     8941 agaatgaaca aacggttgta gaagaaatcg ttcaggcgca agagcctgtg aaggcttctg
     9001 aacaagccgt cgaagagcag ccgcaggcgc atactgaggc cgaggcggaa acttttgctg
     9061 ccgacgttgt ggaagtcact gaacaggttg ctgaaaatga aaaagcgcag cctgaagcgg
     9121 aagtcgttgc acaaccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac
     9181 gtgaagagct gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc
     9241 aggctgaagc ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag
     9301 aaatcaccga cgaagagctg gaagcacagg cgctggctgc cgaagcagca gaagaggcgg
     9361 tgatggtggt tcctccggca gaagaagagc agccggtgga agaaatcgct caggagcagg
     9421 aaaaaccgac caaagaaggt tttttcgcgc gcctgaaacg cagcctgtta aaaaccaaag
     9481 aaaatctcgg ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt
     9541 ttgaggagct ggaagagcag cttttgatcg ccgatgtggg cgtggaaacc acacgtaaaa
     9601 ttatcaccaa tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct
     9661 atggcctgct gaaagaagag atgggtgaga ttctggcgaa agtcgatgag ccgctgaatg
     9721 ttgaaggtaa aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacca
     9781 cgacgattgg taagctggca cgtcagtttg agcagcaggg taaatcggtg atgctggcgg
     9841 cgggcgatac tttccgtgca gcagcggttg aacagcttca ggtctggggg cagcgcaaca
     9901 atattccggt gattgcccag cataccgggg cggattccgc ctctgttatc ttcgacgcca
     9961 ttcaggcggc taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc
    10021 agaacaaatc gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg
    10081 acgttgaagc gccgcatgaa gttatgctga ctatcgatgc cagcaccggg cagaacgcgg
    10141 taagccaggc caaactgttc catgaagccg ttggcttaac cggcatcacg ctaacgaaac
    10201 tggacggcac ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta
    10261 tccgctacat tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact
    10321 ttatagaggc actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa
    10381 ggcttatctc ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga
    10441 gatggcgttt ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg
    10501 tgggattgag cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct
    10561 gaaaaaccgt gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca
    10621 tctactgatg gaccgtactg tctacgataa cgtggcgatc ccgctgatta tcgccggtgc
    10681 cagcggtgac gatattcgcc gccgggtgtc ggcggcactg gataaagtcg ggctactgga
    10741 caaagcgaag aacttcccta ttcagctttc tggcggtgaa caacagcgtg ttggcattgc
    10801 ccgcgcggtg gtgaacaagc ccgcggtact gctggcggac gaaccgactg gtaacctgga
    10861 cgacgcgttg tcggaaggga ttctacgtct gtttgaagag tttaaccgcg taggggtaac
    10921 cgtattgatg gcaacgcacg acatcaacct gatttcacgg cgttcctatc gcatgctcac
    10981 cctgagcgat ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc
    11041 atattcggca gtttggcggg cgtcttgatc gcttccgtaa atcggtcggc ggctcaggcg
    11101 acggcggtcg taacgcaccg aaacgcgcga aatcctcgcc aaaaccggta aatcgcaaaa
    11161 ccaacgtttt caacgaacag gtgcgctatg ccttccacgg cgcattgcag gatctgaaaa
    11221 gcaaaccgtt cgccacattt ttaacggtga tggttatcgc catctccctg acgctgccca
    11281 gcgtctgtta tatggtgtac aaaaacgtta accaggcggc gacgcagtat tatccgtcac
    11341 cgcaaatcac tgtttatctg caaaaaacgc tggacgatga cgccgctgcg ggcgtggtgg
    11401 cacagttgca ggccgagcaa ggcgtagaga aagtgaacta tctttctcgt gaagacgcac
    11461 tgggcgagtt ccgtaactgg tctggttttg gtggcgcgct ggatatgctg gaagaaaacc
    11521 cgcttccggc agtggcggtg gtgatcccga agctcgactt tcaggggacg gagtctctga
    11581 atacgctgcg tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca
    11641 gctggtttgc ccgtctggcg gcgttgaccg ggctggtcgg gcgcgtttcg gcgatgatcg
    11701 gcgtgttgat ggtggcggca gtgttcctcg tcatcggtaa cagtgtacgc ctgagtatct
    11761 ttgctcgtcg tgactccatt aacgtgcaga aactgattgg tgcaacggat ggtttcatcc
    11821 tgcgcccgtt cctgtacggt ggcgcattgc tggggttttc tggcgcattg ttgtcattaa
    11881 ttttatcaga aattctggta ctgcgattgt catcggcggt tgcggaagtg gcacaggttt
    11941 tcggaacgaa gtttgatatc aatggtttat cgttcgatga atgcctgctg ttgctgctgg
    12001 tatgctcgat gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact
    12061 ttacgcctga ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg
    12121 aaagagaccc cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt
    12181 tattcacaag cctgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga
    12241 taacctcgtt gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata
    12301 tcgattgaga ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc
    12361 aacctggatt cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag
    12421 cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc
    12481 ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca
    12541 caggcggatt tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgttttaac
    12601 ccggaagtgg gtgtgcgcct ggtctccttc gccgtccact ggatcaaagc agagatccac
    12661 gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa
    12721 ctgttcttca acctgcgtaa aaccaagcag cgtctgggct ggtttaacca ggatgaagtc
    12781 gaaatggtgg cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggaatcacgt
    12841 atggcggcac aggacatgac ctttgacctg tcttccgacg acgattccga cagccaaccg
    12901 atggcaccgg tgctctatct gcaggataaa tcatctaact ttgccgacgg cattgaagat
    12961 gataactggg aagagcaggc agcaaaccgt ctgaccgacg cgatgcaggg tctggacgaa
    13021 cgcagccagg acatcatccg tgcgcgctgg ctggacgaag acaacaagtc cacgttgcag
    13081 gaactggctg accgttacgg tgtttccgct gagcgtgtgc gccagctgga aaagaacgcg
    13141 atgaaaaaac tgcgcgctgc tatagaagcg taatttccgc tattaagcag agaaccctgg
    13201 atgcgagtcc ggggtttttg ttttttgggc ctctacaata atcaatttcc cctccggcaa
    13261 aacgccaatc cccacgcaga ttgttaataa actgtcaaaa tagctattcc aatatcataa
    13321 aaatcgggta tgttttagca gagtatgctg ctaaagcacg ggtagtcatg cataaaacga
    13381 aataaagtgc tgaacaacaa catcacaaca cacgtaataa ccagaagaat ggggattctc
    13441 aggatgaaca taaagggtaa agcgttactg gcaggatgta tcgcgctggc attcagcaat
    13501 atggctctgg cagaagatat taaagtcgcg gtcgtgggcg caatgtccgg tccggttgcg
    13561 cagtacggtg accaggagtt taccggcgca gagcaggcgg ttgcggatat caacgctaaa
    13621 ggcggcatta aaggcaacaa actgcaaatc gtaaaatatg acgatgcctg tgacccgaaa
    13681 caggcggttg cggtggcgaa caaagtcgtt aacgacggca ttaaatacgt gattggtcac
    13741 ctctgttctt catcaacgca gcctgcgtct gacatctacg aagacgaagg cattttaatg
    13801 atcaccccag cggcaaccgc gccggagctg accgcccgtg gctatcagct gatcctgcgc
    13861 accaccggcc tggactccga ccaggggccg acggcggcga aatatattct tgagaaagtg
    13921 aaaccgcagc gtattgctat cgttcacgac aaacagcaat acggcgaagg tctggcacga
    13981 gcggtgcagg acggcctgaa gaaaggcaat gctaacgtgg tgttctttga tggtatcacc
    14041 gccggggaaa aagatttctc aacgctggtg gcgcgtctga aaaaagagaa tatcgacttc
    14101 gtttactacg gcggttatca cccggaaatg gggcaaatcc tgcgtcaggc acgcgcggca
    14161 gggttgaaaa ctcagtttat ggggccggaa ggcgtggcta acgtctcgct gtctaacatt
    14221 gcgggcgaat cagcggaagg gctgctggtg accaagccga agaactacga tcaggttccg
    14281 gcgaacaaac ccattgttga tgcgatcaaa gcgaaaaaac aggacccaag cggcgcattc
    14341 gtttggacca cctacgccgc gctgcaatct ttgcaggcgg gcctgaatca gtctgacgat
    14401 ccggctgaaa tcgccaaata cctgaaagcg aactccgtgg ataccgtaat gggaccgctg
    14461 acctgggatg agaaaggcga tctgaaaggc tttgagttcg gtgtgtttga ctggcacgcc
    14521 aacggcacgg caaccgatgc gaagtaacag atagataaat gaaaatgccc gatgcgctga
    14581 actgccatcg ggtttttttg tgtttgtcga aatggttgta gaaagattaa acaatccagg
    14641 atatgcttaa tgtacggtga cataccgtac atattatacg gagcaggaga gttcacatgt
    14701 ctgctgttaa aaagcagcgt atcgatctgc gtttaactga cgatgacaaa agtatgattg
    14761 aggaagcggc agcgatatct aatcagtccg tcagccaatt tatgttgaac agcgcttcgc
    14821 agcgggctgc ggaagtgatt gaacagcatc ggcgggtgat cctcaatgag gaatcctgga
    14881 cgcgggtgat ggatgcgctg agtaatccac cgtcaccagg tgaaaagcta aaacgtgcgg
    14941 caaaacgtct tcagggaatg taataattta tggatgatct gacgatagag attctgaccg
    15001 atgatgcaga ttatgatcta cagcgattcg actgcggcga ggaagcgtta aatctctttc
    15061 tgacgacaca tctcgttcgt caacatcgca acaaaattct gcgagcgtat atcctttgtc
    15121 gcaacactcc agaacgtcag gtgctgggat attacacatt atgcggcagt tgttttgaac
    15181 gagccgcatt gccctcgaaa tcgaaacaga aaaaaattcc ctacaaaaat attcccagcg
    15241 ttactcttgg gcgtctggca attgatcgtt cattacaggg gcagggatgg ggagcaacac
    15301 tggttgctca tgccatgaaa gtcgtctggt cagcctcttt agcggtaggt attcacggtc
    15361 tttttgtcga ggcgctgaat gaaaaagccc atacgtttta taaatcgctg ggctttatcc
    15421 ctttagtcgg ggaaaacgaa aatgcgttat ttttcccaac caaatccatt gaactgcttt
    15481 ttacacagag cgattaacgc ttctcccagc cgccctgctg tgccgtaaat cccagtgcct
    15541 gcataaacgc cgtcatcaca ccgcgatctt ccacgcctgc atccgccatc caccagcatg
    15601 aaacgccagg attgttacgc aaaacctctt ccagcagata ttgccccaca ccgcgacgtc
    15661 gagtgacttc ccgcacgcgc agggaatcca gtgccccctc ggtgccgctt aaggttaccc
    15721 gcacggcagc aagcaggcgc tcgttaaaac gcgcggcgta gatgcggtgg ttatcgtcaa
    15781 cctgtaaaga ggaaggggaa tactccggcc agatcttttg caggtcaatc cggtcctggt
    15841 cgctaaattt ttctaatcga atgatggtca gcttcatggg taacccgtgt aaatcacaaa
    15901 agtggaacca gtgtagcgaa ataatttaat cggaggcttt ctctttttta tttcttttgg
    15961 caggtgatta atttcttaac agcaataatt acaaagtt
//
LOCUS       DBFIFS010000096        15969 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.096, whole genome shotgun sequence.
ACCESSION   DBFIFS010000096 DBFIFS010000000
VERSION     DBFIFS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.096"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(264..935)
                     /gene="queE"
                     /locus_tag="N7A07_002695"
     CDS             complement(264..935)
                     /gene="queE"
                     /locus_tag="N7A07_002695"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU2477331.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1090..1710
                     /locus_tag="N7A07_002696"
     CDS             1090..1710
                     /locus_tag="N7A07_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGU2477332.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1724..2398
                     /locus_tag="N7A07_002697"
     CDS             1724..2398
                     /locus_tag="N7A07_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2477333.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2632..3780
                     /locus_tag="N7A07_002698"
     CDS             2632..3780
                     /locus_tag="N7A07_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2477334.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            3777..4691
                     /locus_tag="N7A07_002699"
     CDS             3777..4691
                     /locus_tag="N7A07_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2477335.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4751..6049)
                     /gene="eno"
                     /locus_tag="N7A07_002700"
     CDS             complement(4751..6049)
                     /gene="eno"
                     /locus_tag="N7A07_002700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU2477336.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6137..7774)
                     /gene="pyrG"
                     /locus_tag="N7A07_002701"
     CDS             complement(6137..7774)
                     /gene="pyrG"
                     /locus_tag="N7A07_002701"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU2477337.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(8002..8793)
                     /gene="mazG"
                     /locus_tag="N7A07_002702"
     CDS             complement(8002..8793)
                     /gene="mazG"
                     /locus_tag="N7A07_002702"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU2477338.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8864..9199)
                     /gene="mazF"
                     /locus_tag="N7A07_002703"
     CDS             complement(8864..9199)
                     /gene="mazF"
                     /locus_tag="N7A07_002703"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU2477339.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9199..9447)
                     /gene="mazE"
                     /locus_tag="N7A07_002704"
     CDS             complement(9199..9447)
                     /gene="mazE"
                     /locus_tag="N7A07_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU2477340.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9525..11759)
                     /gene="relA"
                     /locus_tag="N7A07_002705"
     CDS             complement(9525..11759)
                     /gene="relA"
                     /locus_tag="N7A07_002705"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU2477341.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11807..13108)
                     /gene="rlmD"
                     /locus_tag="N7A07_002706"
     CDS             complement(11807..13108)
                     /gene="rlmD"
                     /locus_tag="N7A07_002706"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU2477342.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13165..15921
                     /gene="barA"
                     /locus_tag="N7A07_002707"
     CDS             13165..15921
                     /gene="barA"
                     /locus_tag="N7A07_002707"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU2477343.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
BASE COUNT         3965 a         4052 c         3828 g         4124 t
ORIGIN      
        1 aacacatcca ttatgttaaa gagcacttaa cccattgatt aaaaaggtaa atatttaaaa
       61 taactctata caaactaaaa tctaccaaac tttaccgcaa taattttcac tccagcgaaa
      121 aattaacgcc acagaatttg tagagaatga taatgattag tccgggatag gcaagatgga
      181 tgagttttca gagacgacaa agaatataaa tgagtagaag tctttacagg ttccacccat
      241 ttataacgct tataaatgtt taatcaggca atatttaaat atttatgggt ttgcatcgac
      301 aaacgccaat tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg
      361 ctaatcggct gcagtgcaat gactcgcggt ttatcatcgg tcagcgtcgc caaaagttca
      421 tccagtgctt caatatcacg tacgcgtccc actggatgct tgatttcgtt ggctcgctcc
      481 agtgcctgtg acaacacttc atagccgccg cgcatgttca gctttggcga tacggtaacc
      541 caggtattcg gtgtacagcg tacttcatga gtaccactgg tttcgatctg gcagctaaaa
      601 ccgttctttt cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc
      661 gtaatcacca catgccgcgc ggtatatccc tggcgaccaa tgacagccag caagtcttca
      721 ctgctcgccg ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag
      781 acttcccgat cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc
      841 gggcatccct gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc
      901 agggtctgga acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt
      961 gttaattatt gcagatcctg ccacaacaat catgtcttat taacattctg ttacaggcag
     1021 gtttatggca aaaagtggat tagatcataa agaataaaat gaagcagagt attatgtttt
     1081 aaggaattaa ttaaaaggga tttaaacacg ctcagaatat tcatcgtaat tatatttatt
     1141 ttcaatttat caggctgtgg atataacgat attcaaacct atgatgagca ggttaacgcc
     1201 tcctggtctg aggtattgaa tcaatatcaa cgtcgcgccg atcttattcc taatcttgtc
     1261 gccagcatta aaggttattc cagccatgaa cgagatgtac tggaagccgt aacattggct
     1321 cgtagccagg ctaatcgcgc cagtagcgat cttcagcaaa ctccgggaga tgaacaaaaa
     1381 ttacaagcct ggcaacaggc tcaagcgcag gtgactcgca ctctcggtca gttaacgata
     1441 attagtgagc gttaccctga gttaaaatct caggaactct atcaaaattt aatggtacag
     1501 cttgaaggaa gtgaaaatcg gattgccgtt gcgcgaggaa gatatattaa agccattgaa
     1561 caatataatg tcactatccg caaattcccc gctgtattaa ccgcaaaggt catggattac
     1621 acaccaaaga aaaattattt gccggatgat gtcgccgcag taagtaaagc cccaacaata
     1681 gattttagcc agaatgctaa tgctcattaa gcggggaagt tctatgcgaa attttatctt
     1741 ccttatggct ttcttctgtt catctgtgtt tgccacacaa attccagtac ctgaatcacc
     1801 caagtatgtg aatgacttaa ccggcacatt aacaaacagc gaagtaaaca ccctgaccaa
     1861 ccagattaaa gccctgacgc aaaaaaacta cgcacagttg gtcgtgttag tggttgaaac
     1921 aacaggcgat gaaaccatcg agcagtacgc gacgcgggta ttcgatagct ggaaaccggg
     1981 tgataaagac cgcgatgatg gcgttctact gttagtcgcc tggcaggatc ataccgtgcg
     2041 tattgaaatc ggttacggac tggaaggtat tattacggat gcgcagtccg ggaaaattat
     2101 tcgcaacagt attattcctg catttaaaaa aggcgacctg gctggtggtt tgcaaaaggg
     2161 aatcagcgat attgaaagtc gtctgacggg gaataattta gcaacgataa caccgaccga
     2221 tcaccctttg ccctttagcg gttggtgggc attgttagtc tgggcgatag tactgacgtt
     2281 tatttctgcc agaggatata tcaaaacgct gggagtaatt tgcttcgcag ctattgtact
     2341 ggcctttgtt ttacctatag caggcttcag cggaagctgg ggagtactcg caacatgact
     2401 ctgcttcgcc acaccgttcc tggctgtcgc catcgctttt acccccttcg gcaaaaaagt
     2461 tcgggatttg atgcgtgatg ccaaccagcc atcacgccat acaagaagca attcttcctc
     2521 ccactattca tcatcgtcat cctcttcatt cgacaatgac aattttagcg gtggtggcgg
     2581 ttcatctggt ggcggtggcg cttccgggcg ctggtaacaa aaggaattga tatgcgtatt
     2641 tttctcttac ttctgatgct atgcggtttt caggtattcg ctgaacgatt gccttcagtg
     2701 actgacttaa ccgggacatt aacgacagaa gaacaaactg ctttaactca acaattacag
     2761 actctcgagc agcaaaacca cttccaggtg gccgtgttgg ttacgccgac cacagggggc
     2821 aaaaacatta aacttgcagc ctcgcagaga tacggggtat atcactggaa acccgttgga
     2881 gagaagcgat atggcgaagg tatcttaatt ctcgtcgtat ggccggaagg gctggcgtca
     2941 atgaagattg gacatggact ggaagagatg ttgccgccgg aacaagctgc gcaaattgtt
     3001 cgttaccata tgcagccaga attcgaaaaa aacaatctct ttgctggatt aacgggcgga
     3061 attgaaagca tcgcacagtt tacccatatc aaagcgaacc ttagcccttt ggatgcttta
     3121 gccaatcatc tgtttgctaa tccgcaacgt tcgctgccat gtcttgcctg gacactgctg
     3181 atgatcgttg ccatcgttgt cctctggcta tttgccagcc gcccacggcg tggcatatgg
     3241 atgatatcga tgattacccc tatggtctgg tttttctcct ttcaggacga tgtcatcatt
     3301 cggcgggtat cggtcgtttg ttttagttta ttgttagcaa caacctgcaa gcagcgtctc
     3361 gcggtagttt ttaatatgtg ccgcgtattt cctatgatgc tgaccccaaa aaataaaaac
     3421 gctaaagtga aaaaacaaaa gaagactccg caggttcgtg aatcaggacg aaattcattg
     3481 tttgtcgtga tgctgataat tattgtggga tggtgcctgg tgttcgccag taataaagat
     3541 attgcattca tctcaacaat tattgggtta attgatcatt ttatcggccc aataaccatt
     3601 gctggcatcg ttctgctgat cattgtggcc aaatttactg gcaatcttaa gctggggtca
     3661 ggagaaaaaa gcaacaggaa aacggggaat cgtaatgctc attctcgctc ttcgtcgaga
     3721 aattctttcc gcggtggtgg cggttcttca ggaggcggcg gttcatccgt ccgttggtga
     3781 tcgtaatgcg cttaatcttt attctattca ctttgtggtg ccttcctggg cttgcccagc
     3841 aaattgctgt gccggaactg cgccaacagg tgaccgatat tactggtacg ttaagcactt
     3901 ctgaacaaca atcactgaca cagcaactgc aggatattac acataaaaca cgggcgcagg
     3961 tcgctgtatt agtagtgccg tccaccggag acgattccat tgaacaatat gctacaaggg
     4021 tttttgatag ctggaaactg ggagataagc agagaaacga tggtatttta cttctggttg
     4081 cctgggagga tcatgctgtt cgtattgagg ttggctacgg gctggaaggg gtggttaccg
     4141 acctgcaagc ggcgaagatt atcagggata tattgatccc tgccttcaaa agtgatgacc
     4201 tgatgggtgg attaacactg gcaagcgaaa atatcggcgc gcttctgttg aatggtgaat
     4261 taccggaaga caggggggat tattacagta tcaatccccc tattccatta tcgcttgctg
     4321 taattatatt gctggcggta ctttcatatt ttattgtttt taccgatccg tcgaatttac
     4381 cctggatcac tctcactggc gccatttacg ggatggtatt cctctatgtt gccgagccag
     4441 gaccatggac caatttaatc gttgcctgcg gtatgttaac gccttttgcg atcgtaccac
     4501 tggttatttt ttggcttatc gtcaataaaa agctacgcgc caaatacaaa aaactcagta
     4561 aagatagagc ttcaagaaaa ggttcttctt catcctcttc cggtggcggc tcaagtggcg
     4621 gaggctcagg cggcggattt agcggcgggg gcggttcttc tggcggtggc ggcgcgtccg
     4681 gtcgctggta actcaaccat aaaaaacgcc agcccgaagg ctggcatttt taaatcagat
     4741 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
     4801 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacgcggtca gaacggctca
     4861 tagaaccagt tttgatctgg cctgcagcag taccaacagc caggtcagcg atggtagcgt
     4921 cttcagtttc gccagaacgg tgagagataa ctgcagtgta gccagcgtct ttcgccatct
     4981 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     5041 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     5101 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccatcccagt
     5161 cagattcgtc cagaccgtct tcgatggaaa cgatcgggta ctgtttggtc agttcttcca
     5221 ggaagtgagt gaattcttca gaggtgaatg ctttgttgcc ttcgccagcc agaacgtatt
     5281 taccgtcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     5341 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     5401 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     5461 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     5521 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     5581 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     5641 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     5701 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     5761 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     5821 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     5881 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     5941 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     6001 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     6061 caagtcacta gttaaactga aactccagac aaacaacgcg tacccagggt acgcgttgcc
     6121 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     6181 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     6241 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     6301 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     6361 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtacagctgg cgaaccaggc
     6421 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     6481 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     6541 ccgggtactt acagtctggc acaaattccg tagagttggc gttctccatg ttggcaacat
     6601 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     6661 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     6721 cgccaggtac gaggattgca tccagacctt taaggatttc aacgccgcgc gtttcaacat
     6781 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     6841 gtgcttcaat cacggatttg taagcgtccg gcagttcaat gtacttgccg accataccaa
     6901 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     6961 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     7021 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     7081 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggaacg gcgcgatctg
     7141 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     7201 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     7261 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatcgct tcgaggaacg
     7321 gcagggattc gatatcacct actgtaccgc cgatttcgac cagtactacg tcatgacctt
     7381 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgtacgg
     7441 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     7501 tggtgaagtt gttgcggcgg ctcattttgg tacggatgaa acgctcgtag tgccccaggt
     7561 ccaggtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     7621 tagtacctgg atcgacgttg atgtacggat ccagtttcat gatggtcaca ttgaggccac
     7681 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     7741 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     7801 agacgatgga ataaccagga cgggaaagca gtatacccga acatgacctg tgccacaaac
     7861 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     7921 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     7981 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     8041 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     8101 ctccacttcg cgaaaacgac gctcgaattt ttcgttcgct ttttgcaatg cgatttccgc
     8161 ttttgtccct aaatgacgag ccagattgac cgtggcaaac agcaggtccc ccatttcctc
     8221 ctccagttta gcctggtcga caacagcctg ccgcgcttcg tacatcacct cgtcgatctc
     8281 ttcgtagact ttatcgacta ccggaccaag cgtcgtccaa tcgaagccaa cgttggcgca
     8341 acgtttctgg attttttgcg cacgcattaa agccggtaaa ctacgaggaa tatcgtccag
     8401 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     8461 cacttcacta ctgttttcgg cagaactgtc agcaaaaaca tgcggatggc gacgctctaa
     8521 tttatcgcta atcgcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     8581 ttgggcgtaa aacaccacct ggaacagcag atcgccgagt tcaccgcgca gatcgtcaaa
     8641 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     8701 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccgggtcgc gcaggcgctg
     8761 cataatagtg agcaaacggt cgatttgatt cattagaatg tcctgaaaat tgcgggtccg
     8821 tcaggtggaa acctgtgacc agaatagaag tgagttagta acactaccca atcagtacgt
     8881 taattttggc tttaatgagt tgtaattcct ctggggcaac cgttcctttc ttcgttgctc
     8941 ctcttgcccg ccaggcgata ctttttacct gatcagctaa cgctacgcca tcacgttcct
     9001 gaccggataa aacaacttcg aacggatatc cttttgattg cgttgtacaa ggaacacaca
     9061 gacacatacc tgttttgttg ttgtacatga acggactcag gacaacagcc ggacgatgtc
     9121 cggcttgctc gctacctttt gtcgggtcaa aatcaaccca aatcagatcg cccatatcgg
     9181 gtacgtatcg gcttaccatt accagacttc cttatctttc ggctctcccc agtcgatatt
     9241 ctcgtggagg ttttccggcg tgatgtcgtt gaccagttca gcaagcgtaa atacgggctc
     9301 tttacgcact ggctcaataa ttaatttgcc atccaccagg tcaatcttca cttcatcatc
     9361 aatattcaga ttgagcgcct gcattaacgt agccgggatc cgcaccgccg gtgaatttcc
     9421 ccaacgcttt acgctactgt ggatcataac cctttcctca aaccactatc atatgtagat
     9481 acagtatata tcaatctaca ttgtagatac gagcaaattt cggcctaact cccgtgcaac
     9541 cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg ccccagcact
     9601 tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta
     9661 cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt gatatcacgt
     9721 aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc ggcggagtag
     9781 ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag ttccgccagt
     9841 tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa gccgacaatc
     9901 tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt accaacacct
     9961 tcaaccacta cacgaccgtt atctttactg cggttttgcg gcgtgtagct tttttgctga
    10021 agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa ttgcgattgc
    10081 aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc cagcaactcg
    10141 tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt caggctgatc
    10201 cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg
    10261 tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt gacgtaaccg
    10321 aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat aatttcaatc
    10381 tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg
    10441 atacagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg cgttgatccc
    10501 gcaggcaaat caacgacatc acctttaggc gtaaagacgt acacccggtc gtcaaagacc
    10561 tggctgcgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg ccacgcaatc
    10621 agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc agcagccgcg
    10681 ccctctttat atttccagtg cgcagcaaca cccaactctg catcttcatg catctgtttg
    10741 gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt atgaatagac
    10801 tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg caggtggcga
    10861 tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc ggcgacaata
    10921 cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg cattttgcgc
    10981 cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc ttcagctttc
    11041 atctcagcgc gcagatgacc gacaaactct tcgatatagt gttcgcggtc gagacggcgt
    11101 tcatgcaaca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa
    11161 tcttccagct cccatttcag ttgcccaatc ccaagacggt ttgccaacgg cgcgtagata
    11221 ttggtgcact cttttgccgc cagtacgcgt tcatcttccg gcgcatcttt tacttcacgc
    11281 agatgagcaa tacgctccgc cagtttgatg accacgcagc gaaaatcatc gaccatcgcc
    11341 aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct
    11401 ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac gaccgactta
    11461 ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag agggaacagc
    11521 agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc caccatctca
    11581 acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg cagacaatac
    11641 gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag acttgcgatc
    11701 catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac cgcaaccatc
    11761 gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgcaactat ttaacgcgcg
    11821 agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc atcgccagtc
    11881 gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc gttgcagggt
    11941 tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc ataacacctg
    12001 cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc gcccacggct
    12061 gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt aagcagttaa
    12121 gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca cccaccacac
    12181 tggcagcttg tgtcgccaat ggtagtgtaa agttgcccat accgcagaac agatccagta
    12241 cgcgatcttc aggttccacc tccagccatt ccaacgcacg cgctaccatt ttttggttca
    12301 cacccgcatt aacctgaata aaatcgcgcg ggctaaaagt taagcgcaac ccgtttgagt
    12361 cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg gcgagataca
    12421 gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc gaacttagcg
    12481 gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt tcaacatgac
    12541 caagatggcg catagcttgt aagctgccca ggcatgccct gactttgggc agcaatgctt
    12601 caagttgggg cactaaaatg ggacattgtt taacgtcgac aatgtcactg gagcccgctt
    12661 tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc
    12721 gacggcgata gccccaggga acatcggcga tcacttcaga gacttcgtgt ttcattaatc
    12781 gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt tgttgctgac
    12841 agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg ctatcgctta
    12901 accggcgtac gactttagcg cgggcatact gttttttatc ctcagtaaca gtaacttccg
    12961 cgttttcctg cggcaataat ccggggataa atagcgtttt gccgttatgt cgcgccacgc
    13021 cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc
    13081 gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc
    13141 ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg catgatgatt
    13201 ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt cgtcgtgcat
    13261 cgctataacg acttgcagcg tcaactggaa gatgccggtg ccagcattat tgagccgctt
    13321 gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg tcagttaata
    13381 agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta tgatgaaaat
    13441 aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca gctcggcagc
    13501 aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat gatcctccgc
    13561 acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga tgccaaaaat
    13621 agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt tcgcttgcag
    13681 caatataaag agatctttat ttccagcgtg atgatgctgt tttgtatcgg tattgcgctt
    13741 atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat ggtgaatact
    13801 gtcgaccgca tccgtcgcgg gcaactcgac agccgagtgg aaggatttat gctcggcgag
    13861 ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc ttatcacgaa
    13921 gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct ggagcagatg
    13981 gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt
    14041 aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa tggtgttatt
    14101 ggttttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat
    14161 acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct cgacttctcg
    14221 aaactggaag caggtaagct gattctggaa agtattccat tcccactacg cagcacgctg
    14281 gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga actgacgctc
    14341 aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt acagcaaatc
    14401 atcactaacc tggtggggaa tgcaattaaa ttcaccgaga atggcaacat tgatattctg
    14461 gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat tcgggatacc
    14521 ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg acaggctgat
    14581 gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac acaaaaactg
    14641 gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg ttcaactttc
    14701 tggttccaca ttaatctcga tctgaacccg aacattatta ttgaagggcc atccacccag
    14761 tgcctcgccg gtaaacgcct ggcctatgtc gaaccaaact ccgcagcagc gcaatgcacg
    14821 ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt ctccgcgctg
    14881 cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg cgagccgcta
    14941 acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct gatgctggca
    15001 cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat cggcgcgtgt
    15061 ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac
    15121 aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg
    15181 gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga tatggtgcaa
    15241 catgtggaac tttgcgatag cgggcatcag gcggttgaac gggcgaaaca gatgccgttc
    15301 gatttgatct taatggatat tcaaatgcct gacatggatg gcattcgggc ctgcgagctc
    15361 atccaccagc tcccccatca gcaacaaacg ccggttatcg cggtaacggc gcatgcaatg
    15421 gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc gaaaccgatt
    15481 gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg tatttcgtct
    15541 cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac cctcgactgg
    15601 caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat gctgcaaatg
    15661 ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt tggagaaaac
    15721 ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta tagcggtgtg
    15781 ccgcgtatga agaatctctg ccaacttatc gaacaacagc tacgtagcgg tactaaagaa
    15841 gaagatttgg aaccggagct gctggaactg ttggacgaga tggataatgt cgcgcgcgaa
    15901 gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt tttatgacgc
    15961 tgttttcag
//
LOCUS       DBFIFS010000097        15789 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.097, whole genome shotgun sequence.
ACCESSION   DBFIFS010000097 DBFIFS010000000
VERSION     DBFIFS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.097"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            51..425
                     /gene="yobA"
                     /locus_tag="N7A07_002708"
     CDS             51..425
                     /gene="yobA"
                     /locus_tag="N7A07_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU2477344.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            429..1301
                     /gene="copD"
                     /locus_tag="N7A07_002709"
     CDS             429..1301
                     /gene="copD"
                     /locus_tag="N7A07_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU2477345.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            1314..1655
                     /gene="yebY"
                     /locus_tag="N7A07_002710"
     CDS             1314..1655
                     /gene="yebY"
                     /locus_tag="N7A07_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU2477346.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            2051..2707
                     /gene="pphA"
                     /locus_tag="N7A07_002711"
     CDS             2051..2707
                     /gene="pphA"
                     /locus_tag="N7A07_002711"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU2477347.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(2708..2899)
                     /gene="yebW"
                     /locus_tag="N7A07_002712"
     CDS             complement(2708..2899)
                     /gene="yebW"
                     /locus_tag="N7A07_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU2477348.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(3004..3240)
                     /gene="yebV"
                     /locus_tag="N7A07_002713"
     CDS             complement(3004..3240)
                     /gene="yebV"
                     /locus_tag="N7A07_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU2477349.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(3358..4797)
                     /gene="rsmF"
                     /locus_tag="N7A07_002714"
     CDS             complement(3358..4797)
                     /gene="rsmF"
                     /locus_tag="N7A07_002714"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU2477350.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(4878..7511)
                     /gene="yebT"
                     /locus_tag="N7A07_002715"
     CDS             complement(4878..7511)
                     /gene="yebT"
                     /locus_tag="N7A07_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU2477351.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(7480..8763)
                     /gene="yebS"
                     /locus_tag="N7A07_002716"
     CDS             complement(7480..8763)
                     /gene="yebS"
                     /locus_tag="N7A07_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU2477352.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            8893..9390
                     /gene="msrC"
                     /locus_tag="N7A07_002717"
     CDS             8893..9390
                     /gene="msrC"
                     /locus_tag="N7A07_002717"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU2477353.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            9487..10185
                     /gene="proQ"
                     /locus_tag="N7A07_002718"
     CDS             9487..10185
                     /gene="proQ"
                     /locus_tag="N7A07_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU2477354.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            10205..12253
                     /gene="prc"
                     /locus_tag="N7A07_002719"
     CDS             10205..12253
                     /gene="prc"
                     /locus_tag="N7A07_002719"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU2477355.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            12445..13326
                     /gene="htpX"
                     /locus_tag="N7A07_002720"
     CDS             12445..13326
                     /gene="htpX"
                     /locus_tag="N7A07_002720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU2477356.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(13372..14745)
                     /gene="yebQ"
                     /locus_tag="N7A07_002721"
     CDS             complement(13372..14745)
                     /gene="yebQ"
                     /locus_tag="N7A07_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477357.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            14922..15713
                     /gene="kdgR"
                     /locus_tag="N7A07_002722"
     CDS             14922..15713
                     /gene="kdgR"
                     /locus_tag="N7A07_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU2477358.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
BASE COUNT         4098 a         3881 c         4080 g         3730 t
ORIGIN      
        1 agacaaggtt atgctctgta aaaaagttat tacaaaaagg aatagataat atggcttcaa
       61 ctgcacgctc ccttcgctac gcgctggcaa tcctgaccac ttcgttggtg acaccttccg
      121 tctgggcaca tgcgcattta acgcatcagt atcctgcggc aaacgcgcaa gtgacagctg
      181 caccgcaggc aatcacttta aacttctcgg aaggtgttga aacaggattc agtggtgcaa
      241 aaatcacggg gccaaaaaac gaaaatatta aaacattacc ggcgaagcga aatgagcagg
      301 accaaaagcg actgattgtt ccactggccg attcgctgaa acccggaacc tacaccgttg
      361 actggcatgt tgtttcggtg gacgggcata aaacgaaagg acactacacc tttagcgtga
      421 aataaatcat gctggcgttt acctggatcg cgctgcgatt tatccatttc acctcgctga
      481 tgctggtctt tggcttcgca atgtacggtg cctggctggc acccttaacg attcgtcgct
      541 tgctgacgaa gcgttttctg cgtttacaac agcacgctgc cgtctggagt ttgatcagtg
      601 ccactgcaat gctcgccgtt cagggcgggt tgatgggaac gggatggaaa gatgtatttt
      661 cgccaaacat ttggcaggct gttttgcaaa cgcagtttgg tggcgtctgg ttatggcaaa
      721 ttgttctcgc cctcgtaacg ttgatcgtcg cccttatgca accgcggaat atgccacgtt
      781 tgctgtttat gctcactacc gcgcaattta ttctgctggc gggagtgggg catgcgacgc
      841 tgaatgaagg ggtaacggcg aaaatccatc agactaatca cgcgattcac ctgatttgtg
      901 cggcggcgtg gtttggggga ttactgccgg tgctctggtg tatgcaactc atcaaaggcc
      961 gttggcgaca ccaggctatt caggcgctga tgcgtttttc ctggtgcggg cattttgcgg
     1021 tgatcggcgt actggcaagc ggcgtgctta atgcattgtt gattaccgga tttcccccca
     1081 cgcttacgac ttactggggc caactcttat tgctcaaagc catcctggtg atgattatgg
     1141 tggtcattgc gctggctaat cgttatgttc ttgtaccgcg catgaggcag gatgaagatc
     1201 gtgcggcacc ctggtttgtg tggatgacta aactcgaatg ggcgataggt gcggtagtgc
     1261 tgctgatcat cagcctgctt gcgactctcg aacctttttg atggattacg aaaatgatga
     1321 aaaaaagtat tctggcgttt ctgttactca ccagttctgc agcggcgctg gctgcaccgc
     1381 aggtgattac cgtcagccgt tttgaagtgg gtaaagacaa atgggcgttt aatcgcgaag
     1441 aggtgatgct gacttgccga ccgggtaatg ctttgtatgt catcaaccca agtaccctcg
     1501 tgcagtatcc tttaaacgat gtcgcacaga aggaagttgc cagtgggaag actaaagccc
     1561 aacccatttc ggtgattcag attgatgatc ccaacaatcc cggcgaaaaa atgagtctgg
     1621 caccgtttat agaacgagct gaaaaactct gttaattacc taaaatagcc ttttgatttc
     1681 caataaaaaa accgcctcag ttctttcacc agaacgggcg gtttttaaca tttaagctga
     1741 tgaccaccgc gctttttatt gaccattttg cacgcaaact ggaaaacctg gcgtcgtcat
     1801 ctattcttaa agggcaaggc aactaagcct gcattaatgc caacttttag cgcacggctc
     1861 tctcccaaga gccatttccc tggaccgaat acaggaatcg tgttcggtct ctttttatct
     1921 gttaaaagcc agaagcattt ccttcgctga ctttatagtc aaccataaca cacactctac
     1981 tgtctgagtc cagcgttttt taacattctt gttaagatta tgtgatcttt agcgcgggag
     2041 gaaaatattg atgaaacagc ctgcgcccgt ttatcagaga attgcgggtc atcaatggcg
     2101 acatatctgg ctttctggcg atatacacgg ttgtcttgag cagttgcgcc gcaaattatg
     2161 gcattgtcgt tttgatccgt ggcgagattt acttatctca gtgggagacg ttatcgatcg
     2221 tgggccgcaa agtttacgtt gtctgcagtt actggaacaa cattgggttc gtgcggtaag
     2281 aggcaatcat gaacagatgg cgatggatgc gctggcatcc cagcagatgt ctttgtggtt
     2341 gatgaatggc ggcgactggt ttattgcgct ggcagataat caacagaaac aagcgaaaac
     2401 ggcgctggaa aaatgtcagc atttgccctt tattcttgaa gtacacagcc gcaccggcaa
     2461 acatgttatt gctcatgccg attatccaga tgatgtttat gaatggcaaa aggacgttga
     2521 tttgcatcag gtcttgtgga gccgctcgcg attaagtgaa cgccaaaaag ggcagggaat
     2581 tacaggtgct gatcatttct ggtttggtca tacaccgttg cgccatcgcg tggatattgg
     2641 caacctgcat tatattgata ccggcgctgt ctttgggggc gaactgactt tcgtgcaatt
     2701 gcaataatta aaaatcacca tactcctgtg ctggtcgcca gaaaccatct ataaaatcct
     2761 caatcggaaa acaaccgcca tgacggatcc gttgatcgct catagaataa agacactgct
     2821 gttccgtgtt gtagacatcc acaacaatat cttcacaacc gccatccagg tagcaaacaa
     2881 aaagtaccag cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag
     2941 agattttaca acgggggaat aaccaggaca aataacccgc catgccgacg ggttcttttt
     3001 ggatcaggca agacgcataa tccaggcatc agattgttga tcgtaacggg tacggtaaag
     3061 gacagagtgt tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag
     3121 ttgcatacat aaatcggggt ctgatttaca aggaatggtt aacgttcgat cacccggcga
     3181 ttcaccgtgt aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat
     3241 cttcgtgctc tccatttagc tgccggttgt ggtacgacca gtccgatcgc accatttttc
     3301 agcctagttg aggccgccga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca
     3361 tgcgttactg gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag
     3421 tcgcgaacca atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc
     3481 atccgccact ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctccgcttc
     3541 ttgcggtgtc agttcaaaag cgttctcgtt gtcgggggag gcaagggcaa taaccgcttc
     3601 atgctgccag cgataacctt tgttgtgcgt ttcggcaagt ttaatcccca ggcgagaaaa
     3661 tcggacttta ccgatcaggg cttcaatgcc taccggaaac aaccacactt ctttgtcgcg
     3721 ctgccagagt cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat
     3781 ttgtgcggct tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg
     3841 ggtgggtaag gcgggaatgg cctgagtttt acgcagacga gcaacgaaga agccttcgca
     3901 gtcgtaaatt tgtgggaaaa catgcaaaaa gccttcttcg gtcagcgctt tgtttgcacc
     3961 agggaagaga tcgccaagtg gtaaaaactc tactgcgtcg gggtaagtct ctttaagcca
     4021 caggcaaacg gattcgtttt cttcccggtt taaggtacag gtcgagtaaa ccagtgtacc
     4081 gccagagcgt aatgcatgaa aggcactgtc gatcagttcc cgttgggtag ctgcgatttc
     4141 ctgattgctt tctggtgacc aattttttag cgcatcggga tctttacgca ccacgccttc
     4201 gccggagcag ggagcgtcca gcaaaatggc atcgaacatt tctggcaatg ccgcaccaaa
     4261 cacgcggcca tcaaaatgtg tgagcgcaac attactgata ccacagcggc tgatattggc
     4321 atgtaacact tttacccgac tggcggaaaa ctcattggca aggattgccc cttcgttatt
     4381 catccgcgcg gcaatttgcg tcgttttgga gcctggcgca gcagcgacat ccatcacccg
     4441 ctgtggtgca ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg
     4501 aatataaaac aggccactta aatgctcggc ggtactaccc aatggcaatg catcttcatc
     4561 gtcgcgttca atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata
     4621 aggagcggtt aatcgcagga aatcagcaac ggaggttttc agcgtattaa cgcgaatgct
     4681 gcggcgcaac gggcgctgac aggcggcaag aaaatcatca aaagagagcg tcgaaggcat
     4741 tgcttcgcgc atttgcgtca gaaaggcgtc cgggaaataa acggtgtgtt gggccacgag
     4801 catataccac tggcaaaaaa aacaggcgcg cagtttagca taaacgctcc ggcgcaggca
     4861 cgccggagca gtgggcatta tttgggaagc gcagttcccc attcacgcca ctctttcggt
     4921 tcactttcct gcaacaggaa gtgcttgcct tcctgggctt tcggtgccag tggcgtaccc
     4981 ggaggcgtgg cgaaggcgat gccgccacgg ataaactggt taaaggtgcc ggttttcact
     5041 acgccgcccg tcagaccaaa gtccagactg taacccgatg ccaaccagaa gacggaattg
     5101 ttacgcacca ggtgttgata gcgtttactg atgcgcatcg caatcatcac gcgatctgac
     5161 aatgtcccca gcgtcattcc ggtaaccgta ccgacttcca gaccacggaa cagcacaggc
     5221 gtaccgatgc ctaacgaaca ggcttccggc gcttcaacaa taatgcttaa accatccagg
     5281 taacgcgaat cagtaatggt ggcctcttgt aattcaaagt cgcgacgagg attgccacgg
     5341 cctggttcga cgttgatata cggctggagg atagtatcaa gatgctcaac gccagctgcc
     5401 gaaatttgcg gtgtgaccac tgagaagcgc gtaccaccgc gagcaaaggt ctggacatat
     5461 tccggataga gcaccgcctt tgcctgtact tcattgcgcg cggtaatcag atccagcgtc
     5521 tggatttgcc cgatatcaat accgagatag cgaattggca tcccgaccgc cagttttccg
     5581 gcatcgaaag catgaagcgt aatctgcccg ccaaccgcac gggccgctgt ttcggaagca
     5641 tacagaatac gcttatcgcc tttacgctga ctggcgctgg caccgctgag gttatcgaag
     5701 ctaatggctc cctttaatgc tctggagagt ggggatgcct gtacggtcag gccactacca
     5761 ttcagctgaa ctttcgcccc gccttctgcc cagaacacgc tattgttggt cagaaggttg
     5821 cgatactccg gcttaatatg cagatcgata tcaaacgcgt tagctcgcgg acgcacggta
     5881 ataacttcac caacttcaaa tttacggtac agcactaccg atcctgcctg cacatccggc
     5941 agcgtctctg cactcaaact cacggttgtg gtgggtaaat cgctaaggct gttctccagc
     6001 gctttttcca gattggcata cagtggatag ctggctttca tctcaccttt atcgcccggc
     6061 agaatacgta tcccgccatt gatccattct gaggcgctgg caccgagaaa ctcaacgcca
     6121 tccagcccca ccttcacatc gacacggctg ttgacgacaa atttgctatc gccttttacc
     6181 agttcgcgat gctgaggctc aatggcgacg gtaaaggtaa cgcctttgct ggtgagttta
     6241 cgatcaataa cctggcctac ctgcacgccg tgaagaatga gcggctgacc agcatcaata
     6301 ccgtaacttt ccggtgctgt cagggtcagt gtcagaacat caggttcatg cagcagtgct
     6361 ttttcgcctg gcacaacaac gaactctttg cgtggctcgc catcgccagg caccaactca
     6421 aaggtttttc cggtcagcag ggcgctgaga ttggcatcgc taagggataa tttcgggttg
     6481 cgtaattcga tgcgggtatt ttcccgtaac agcgtaacga cgctgggatc aacggtcatt
     6541 tctccggtga ctttaccacc aggatttaaa tccagtttag tcagctgtcc gacttccagc
     6601 ccctgataca ttaacggcgt cgagtcggcg gttaatccgg ccccactcgg cagttccagt
     6661 tttattatta cgccacgctg gctgtgggcc agatcttcat acagaccaaa ggtatcttcc
     6721 gcctcggcag gtttcgactc ttctggtgaa tcgaaggcaa tcgcaccgtt aaccagtgcc
     6781 gccagacttt ccagtttcac cttcgcgcca ctgatactga cgttggcatc aacgcctgaa
     6841 acgttccaga aacggctacc ttttttcacc aggtcggtaa aacgccgctc gatcaggaca
     6901 tcaatcacca cgccttgttt attgggattg atggcatagt cgtagacttt tcccaccggg
     6961 atcttgcgga aatagaccaa tgaaccgctg ttcagcgaac cgagatcggg agcttgcagg
     7021 tggatcatca gatcgccgtt atccagccga tacttcggtt gggtatcgag cgcgacaaag
     7081 tgatcctgct ctttaccttt acctggcatc atgccgatat agttaccacc gacgagggcg
     7141 tccagcccgg agacacctgc caacgatgct tttggcgtca ccagccagaa ctgagtctct
     7201 tcgcgcagcg catctttcat atcggacttg atgctgacct tgacttcaat cttacgaaga
     7261 tcgtcgctga ggctgatatc ctgcactgtt ccgacttcaa cgccctgata acgaacaggc
     7321 gtacggcccg ggacaatacc atccgccgac ataaagtcga tggtgacggt attaccccgg
     7381 tcctgataac tgtcccaaat cagccaaccg gcaatcatta gcgcgatgaa cggcagcagc
     7441 cagaaaggtg agatacggcg tttattttta atctgcgctt cagtcgtcga agcgggcgtt
     7501 tcctgactca tgtgcatccc aaagtaagcg gctgtccagc cattccacag caagaatagt
     7561 caaaattacc gctgcgccga aataaaacgc agccggtccc atagtaaaag cgaggatctg
     7621 atcgcgatta atcagcgaca tggttaaaga tatgacaaac aggtcgagca tcgaccagcg
     7681 accaatccag gtcaccatcc gcagtaacag aatgcgtgtg cgtaaacctt gctggcattt
     7741 aaaatgaatg ctcaacagta aagtgaacat gacgatcact ttagtaaacg gtaccagaat
     7801 actggcgata aacacgattc ctgcaaccgc aatgttgcta cttgccagcg acataattcc
     7861 ggaaagaatt gtatcttcct gccgtcctcc gttcagataa atgatagaaa taggcaacag
     7921 gttggcaggt aacaataaaa cgattgacgc taacagcgcc gcccagcatt tttgcagact
     7981 atgacggcga cgcaggcgta gcggaatatg acaacgcggg cagcgaccac gctgatcagg
     8041 atagccggta aaatggcacc caagacagac acgaagtttc tcgtccctac gcgtagcggg
     8101 gcgctgcgga taaaatcgct cccacagctc ctcgacatta agatgtgaca acgtcaccgt
     8161 cgttaaaatc accaacgcca caaaagagaa caagccgaca cccgcctgga tatgggcata
     8221 atcctgtacc tttatagaag caacgccaat gccgaccagg tagatatcga gcattaccca
     8281 ctctttaagt cgctcaagca tcagcagtac cgggcgtaga ttcattccca gtcggttacc
     8341 aaaccataaa taagctatgg aggtcaccag aatgagcggg gcaccaataa cgcagaaaaa
     8401 gaccatcgac cccgttatcg catcgccctg tttggtcatt tgccagatgc cttgcataac
     8461 gttggcgtca atacgaatgc ctaacagcca gatatgcaac agcggttcgc cccaggcaaa
     8521 cggcatcagc aacagcatgg tgaaggccat tgccgccagg cgcgttagcg accagtcgcg
     8581 cccgtcacga atttttgcct gacagcgcgg acaataggca ctttgatgag aatttatctc
     8641 gggcaggcta aacagcatgt cacattgcgg gcaacgttgg taatcaccac gcggcagttc
     8701 ctcgccgatt gccctcactg ttatcttttt tgtcggcgta atttgtggtg tgttaagagc
     8761 catgacgtgc caaatgtaat aagaattaac attaatctta acccatgatg ggaatgatct
     8821 tgagcattaa cgcatttaat gcttatttta ataggctggc agttccaggt aagacaacca
     8881 agtgattata taatgaacaa aacagaattt tacgcggatt taaatcgcga ctttaacgcg
     8941 ctgatggcgg gagaaaccag ttttctggca acgcttgcga acaccagtgc gttgttatat
     9001 gagcgtctca ctgacgtaaa ctgggcaggt ttttatttgc ttgaggacga tacactggtg
     9061 ctcggaccat ttcagggcaa aattgcctgt gtccggatac ccgtcgggcg tggcgtgtgc
     9121 ggcactgcgg ttgcccgcaa tcaagtgcag cgtatcgagg atgttcatgc gtttgacggg
     9181 catattgcct gtgatgcggc gagtaattct gaaattgttc tgccgctggt ggtgaaaaat
     9241 cagattattg gtgttctcga catcgatagt accgtcttcg gtcgctttac agacgaggac
     9301 gagcaaggct tacgtcagct tgtggcacag cttgaaaaag tgcttgcaac gacggattac
     9361 aaaaaattct ttgcgagcgt cgcaggataa tcaacggata acgtagcatt tactgatagc
     9421 gtcattataa tgacgcctgt tcatgcctgc gcttgttggc tacgtccgtt gtaatcagga
     9481 aatttcatgg aaaatcaacc taagttgaat agcagtaaag aagtaatcgc gtttctggcc
     9541 gaacgttttc cccactgttt cagtgcggaa ggtgaagcgc gtccgctgaa aatcggtatt
     9601 tttcaggatt tggtcgatcg tgttgccggg gaaatgaacc tgagcaaaac gcaattacga
     9661 tccgctttac gtctctacac ttcgagctgg cgttaccttt acggtgttaa acccggcgca
     9721 acgcgtgtcg atcttgacgg caacccatgc ggtgagctgg acgagcaaca tgtagagcat
     9781 gctcgcaagc agcttgaaga agcgaaagcg cgtgttcagg cacagcgtgc tgaacagcaa
     9841 gcgaaaaaac gcgaagctgc cgcagctgct ggtgagaaag aagacgcacc gcgtcgcgaa
     9901 cgcaagccac gtccgaccac gccacgccgc aaagaaggcg ctgaacgtaa acctcgtgcg
     9961 caaaagccgg tagagaaagc gccaaaaaca gcaaaagcac ctcgcgaaga acagcacacc
    10021 ccagtttctg acatttcagc tctgactgtc ggacaagccc tgaaggtgaa agcgggtcaa
    10081 aacgcgatgg atgccaccgt attagaaatc accaaagacg gcgtccgcgt ccagctgaat
    10141 tcgggtatgt ctttgattgt gcgcgcagaa cacctggtgt tctgaaacgg aggccgggcc
    10201 aggcatgaac atgtttttta ggcttaccgc gttagctggc ctgcttgcaa tagcaggcca
    10261 gaccttcgct gtagaagata tcacgcgtgc tgatcaaatt ccggtattaa aggaagagac
    10321 gcagcatgcg acggtgagtg agcgcgtaac gtcgcgcttc acccgttctc attatcgcca
    10381 gttcgacctc gatcaggcat tttcggccaa aatctttgac cgctacctga atctgctcga
    10441 ttacagccac aacgtgctgc tggcaagcga tgttgaacag ttcgcgaaaa agaaaaccga
    10501 gttaggcgat gaactgcgtt caggcaaact cgacgttttc tacgatctct acaatctggc
    10561 gcaaaagcgt cgttttgaac gttaccaata cgctttgtcg gtactggaaa agccgatgga
    10621 tttcaccggc aacgacactt ataaccttga ccgcagcaaa gcgccctggc cgaaaaacga
    10681 ggctgagttg aacgcgctgt gggacagtaa agtcaaattc gacgagttaa gcctgaagct
    10741 ggcaggaaaa acggataaag aaattcgtga aaccctgact cgccgctaca aatttgccat
    10801 tcgtcgtctg gcgcaaacca acagcgaaga tgttttctcg ctggcaatga cggcgtttgc
    10861 gcgtgaaatc gacccgcata ccaactatct ttccccgcgt aataccgaac agttcaacac
    10921 tgaaatgagt ttgtcgctgg aaggtattgg cgcagtgctg caaatggatg atgactacac
    10981 cgttatcaat tcgatggtgg caggtggtcc ggcagcgaag agtaaagcta tcagcgttgg
    11041 tgacaaaatt gtcggtgttg gtcaaacagg caagccgatg gttgacgtga ttggctggcg
    11101 tcttgatgat gtggttgcct taattaaagg gccgaagggc agtaaagttc gtctggaaat
    11161 tttacctgct ggtaaaggga ccaagacccg cactgtaaca ttgacccgtg aacgtattcg
    11221 tctcgaagac cgcgcggtta aaatgtcggt gaagaccgtc ggtaaagaga aagtcggcgt
    11281 gctggatatt cctggcttct atgtgggttt gacagacgat gtcaaagtgc aactgcagaa
    11341 actggaaaaa cagaatgtca gcagtgtgat catcgatctg cgtagcaatg gtggtggggc
    11401 gctgaccgaa gcggtatcgc tctcaggtct gtttattcct gcgggtccta ttgttcaggt
    11461 ccgtgataac aacggtaaag ttcgtgaaga cagcgatacc gacggacagg ttttctataa
    11521 aggcccgctg gtggtactgg ttgaccgctt cagtgcttcg gcttcagaaa tctttgccgc
    11581 ggcaatgcag gattacggtc gtgcactggt tgtgggtgaa ccgacgtttg gtaaaggcac
    11641 cgttcagcaa taccgttcat tgaaccgtat ttacgatcag atgttacgtc ctgaatggcc
    11701 agcgctgggt tctgtgcagt acacgatcca gaaattctat cgcgttaacg gcggcagtac
    11761 gcaacgtaaa ggcgtaacgc cagacatcat catgccgacg ggtaatgaag aaacggaaac
    11821 gggtgagaaa ttcgaagata acgcgctgcc gtgggatagc attgatgccg cgacttatgt
    11881 gaaatcaggc gatttaacgg cctttgaacc ggagctactg aaggaacata atgcgcgtat
    11941 cgcgaaagat cctgagttcc agaacatcat gaaggatatc gcgcgcttca acgctatgaa
    12001 ggacaagcgc aatatcgttt ctctgaatta cgctgtgcgt gagaaagaga ataatgaaga
    12061 tgatgcgacg cgtttggcgc gtttgaacga acgctttaaa cgcgaaggta aaccggagtt
    12121 gaagaaactg gatgatctac cgaaagatta ccaggagccg gatccttatc tggatgagac
    12181 ggtgaatatc gcactcgatc tggcgaagct tgaaaaagcc agacccgcgg aacaacccgc
    12241 acccgtcaag taatatcaat caggcacaag aaattgtgcc tgatttttta acagcggcaa
    12301 gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga cttacgcact
    12361 atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata ttgcgttttg
    12421 ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct aacgaacctg
    12481 gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc gagcagcgtt
    12541 caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt ttcgcttctg
    12601 atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca accgcgtaac
    12661 gaaagggaac gttggctggt caatactgta gcaacccagg ctcgtcaggc ggggatcgct
    12721 atgccgcaag tggctatcta ccatgcgccg gacatcaacg cttttgcaac cggtgcgcgc
    12781 cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag cccggatgaa
    12841 gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat ggtcaccatg
    12901 acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat cctggcgcag
    12961 cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa cggcaacccg
    13021 ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct ggcgagcatt
    13081 atcaccatgt ggttctcgcg tcatcgtgaa ttccatgctg atgccggttc ggcaaaactg
    13141 gttggtcgcg agaaaatgat tgccgcgctg cagcgcctga aaaccagcta tgaaccgcaa
    13201 gaagcaacca gcatgatggc tttctgcatt aacggtaagt cgaaatcgct cagtgagttg
    13261 tttatgactc acccgccgct ggataaacgt attgaagctc tgcgtacggg tgaatacctg
    13321 aagtaatatg aagtaaatac taaaaagcgc gtcctgctcg acgcgctttt tttatgccct
    13381 ggatcttggc tgagtgatac gtaacccact gatacaggca gcaatcactg ccagaatagc
    13441 cgcagccatc agcgagacgt gtgtaccatt atctccaaac tgatttagca tcagcgccac
    13501 cagcgccgcg ccgctactct gacccagtag acgagccgtt cctaacatgc cactggctcc
    13561 accgctacgt tcgcgaggcg cggaggtaat aatggtgtgg ttattgggtg actggaacaa
    13621 gccaaatcca gcgccacata agatcatcgg ccagataata ttgatatccg caggtgacgc
    13681 gggcagcaga accagggaaa aaagccccgc agccatgatg aataatccta aagcacccag
    13741 caatcctgca tgtacgcgtt caatcaaata gcctgccaac ggagccatca ccatcgttgc
    13801 taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga gcacggtttg
    13861 caggtaaaag ggcagggaaa ccattgccag catttgtgcg cagaaagagc aaacagatgt
    13921 gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca gcggtactgg
    13981 aagagaaagc tggcggcgaa taaagaaaat accaacgaca accattacaa ccagttccgc
    14041 agcaattaac gtcagcgatt gcccctgagc gaaaccactc aacgcagtga taagcaggcc
    14101 gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac tggcgcgaga
    14161 accattaggt ggcagaaaac gcatcgccag aagcagggcg ataataccca acggtacgtt
    14221 gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa ttgtcggccc
    14281 ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctac ccagaaaacg
    14341 ttgtggatag atcaggcgga taagggcggt attaacgctc atcaacgctg caccgccgaa
    14401 accttgtatg acacgcgcaa gggtgagtat ttgcagcgaa tcagaaaggg cgcagaacag
    14461 tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc caaacatatc
    14521 acccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg cgttcactac
    14581 ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca gggcgacgtt
    14641 ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta atatcgcacc
    14701 gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa ttttaaatct
    14761 ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg atgtcgcaga
    14821 attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt ataataaaaa
    14881 acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac gcagatctgg
    14941 ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg caggcgctgg
    15001 gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg cgtcatgatg tcaaaaagca
    15061 ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag gaaggggagt
    15121 cggagaaata ttccctgacc ctgaaattgt ttgaactggg cgctcgcgcg ttacaaaacg
    15181 tcgatttaat tcgtagcgca gatatccaga tgcgtgagat ctcccgcctg accaaagaaa
    15241 ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa attgactcta
    15301 tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccactgtac agcaccgcga
    15361 ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gaagcaaatt cttgagggcg
    15421 tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta ttaccggttc
    15481 tggaccaggt gcgcgagcag gggtatggcg aagataacga agagcaggaa gaagggctgc
    15541 gatgcattgc ggttccggta tttgatcgct ttggcgtggt cattgccggt ttgagcatct
    15601 ccttcccgac gttgcgtttc tctgaagagc gtttacagga atatgtcgca atgttgcata
    15661 ccgcagcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc tgatgagagt
    15721 aagaacctgt cggaatatca aacagacagg ttctttattt agcatgagaa aaataaagtt
    15781 gaaggtggc
//
LOCUS       DBFIFS010000098        15664 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.098, whole genome shotgun sequence.
ACCESSION   DBFIFS010000098 DBFIFS010000000
VERSION     DBFIFS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.098"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..601
                     /gene="ais"
                     /locus_tag="N7A07_002723"
     CDS             <1..601
                     /gene="ais"
                     /locus_tag="N7A07_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU2477359.1"
                     /translation="LAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKT
                     LGRLAQQHPVVVLFRHAEHCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFDL
                     YSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNHC
                     LTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(640..1065)
                     /gene="nudI"
                     /locus_tag="N7A07_002724"
     CDS             complement(640..1065)
                     /gene="nudI"
                     /locus_tag="N7A07_002724"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU2477360.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1344..1886
                     /gene="yfaZ"
                     /locus_tag="N7A07_002725"
     CDS             1344..1886
                     /gene="yfaZ"
                     /locus_tag="N7A07_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU2477361.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1986..3188
                     /gene="yfaY"
                     /locus_tag="N7A07_002726"
     CDS             1986..3188
                     /gene="yfaY"
                     /locus_tag="N7A07_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU2477362.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3408..4190
                     /gene="yfaX"
                     /locus_tag="N7A07_002727"
     CDS             3408..4190
                     /gene="yfaX"
                     /locus_tag="N7A07_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGU2477363.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4205..5410
                     /gene="rhmD"
                     /locus_tag="N7A07_002728"
     CDS             4205..5410
                     /gene="rhmD"
                     /locus_tag="N7A07_002728"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HGU2477364.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5467..6756
                     /gene="yfaV"
                     /locus_tag="N7A07_002729"
     CDS             5467..6756
                     /gene="yfaV"
                     /locus_tag="N7A07_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477365.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6782..7576
                     /gene="yfaU"
                     /locus_tag="N7A07_002730"
     CDS             6782..7576
                     /gene="yfaU"
                     /locus_tag="N7A07_002730"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGU2477366.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7617..8543)
                     /gene="rpnE"
                     /locus_tag="N7A07_002731"
     CDS             complement(7617..8543)
                     /gene="rpnE"
                     /locus_tag="N7A07_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU2477367.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(8736..9926)
                     /gene="glpC"
                     /locus_tag="N7A07_002732"
     CDS             complement(8736..9926)
                     /gene="glpC"
                     /locus_tag="N7A07_002732"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU2477368.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9923..11182)
                     /gene="glpB"
                     /locus_tag="N7A07_002733"
     CDS             complement(9923..11182)
                     /gene="glpB"
                     /locus_tag="N7A07_002733"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU2477369.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11172..12800)
                     /gene="glpA"
                     /locus_tag="N7A07_002734"
     CDS             complement(11172..12800)
                     /gene="glpA"
                     /locus_tag="N7A07_002734"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU2477370.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13073..14431
                     /gene="glpT"
                     /locus_tag="N7A07_002735"
     CDS             13073..14431
                     /gene="glpT"
                     /locus_tag="N7A07_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU2477371.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14436..15512
                     /gene="glpQ"
                     /locus_tag="N7A07_002736"
     CDS             14436..15512
                     /gene="glpQ"
                     /locus_tag="N7A07_002736"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU2477372.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3635 a         3837 c         4241 g         3951 t
ORIGIN      
        1 gttagctttt tgccgctcat cattgaagtc aaaaaaatat ttcatcattt tactggcgct
       61 cgctgcaatt gccggactgg gtactcatgc cgcctggagt agcaatggtt tgccacgtat
      121 cgacaataaa acgctgggca gactggcaca gcagcacccg gttgtcgttt tgtttcgcca
      181 tgctgaacat tgcgatcgtt caaccaatca atgcttgtca gataaaacag gtattacggt
      241 taaaggtacc caggatgccc gtgaactggg caacgctttt agtgctgata tccctgattt
      301 cgatctttat tccagtaata ccgtccggac cattcagtcc gcgacctggt tttcagcggg
      361 taaaaaattg acggtagata aacgacttct tcagtgcggt aatgagattt atagtgcgat
      421 taaggactta caaagcaaag cgcctgataa aaatatcgtt attttcaccc ataatcattg
      481 cctgacatat attgctaaag ataagcgtga tgcgacattt aaacctgatt atctggatgg
      541 tttagtcatg catgtggaaa aaggcaaagt ttatctggat ggggaattcg ttaatcacta
      601 aattttcaat ctgacagcca gtaatggctg tcatcattgt tacagaagac ctttcaaacg
      661 taacgttttt cgggtggcga cattcaaatc atagtgcgcc agatcttcag gttttaccca
      721 cgcgtagtcc tgaaactctt cgtttatttt cacttctcgg ttggcagaaa cgcaatcaaa
      781 aatcaggtaa atcatataaa tttcttcctt gcgaccatct gcatacgtct tggtgcgaat
      841 atcatcgctg aaggtccagg gcgtgatttc tgtcaaaagc agctgttctc ccagttcttc
      901 gcgaatttcg cggcgtagag cctcttcaat tcgttcgccg ggctccacgc cgccacccga
      961 aagcgcccat tgaccgggga aaacgccgcg atcgtcggcc attttacaca gcaaataagc
     1021 accatcattt tgaatcaaag ggcatacaat agtccgttgt cgcacagggg tattccttat
     1081 aaataaaaag aaaccatgag aatacggtaa ctcagaaata tgtgaaagtg acagtaatct
     1141 cttttgcgat gataactgca cttattatta tttttgtgtg tatcggtaaa ctgtgtttat
     1201 tttttcggcg tttaattatt taagaagtat ttagttacaa taagtgacta gatccctttt
     1261 ttcgctcatt gaatcctctg cgccaggcag tacaatcccg cagctttggg aattttgatt
     1321 aattgcttgt gggaaagggg ataatgaaaa aaattgcgct agcaggtctg gccggaatgc
     1381 tgttggtttc tgcatcggtc aatgcaatga gcatcagcgg ccaggcgggt aaagaataca
     1441 ccaatattgg tgtcggtttt gggactgaat cgacgggcct ggctttaagc ggtaactgga
     1501 cacataacga cgacgacggt gacgtcgcgg gcgtggggct ggggttgaat ctgcctctcg
     1561 ggccgttaat ggcgaccgtt ggcggaaaag gcgtttacac caacccgaat tacggcgatg
     1621 aaggctatgc cgcagcggta ggaggtggtt tgcagtggaa aattggcaac agcttccgtt
     1681 tgtttggcga gtattactac tctccggatt cactctccag cggtattaaa agttatgagg
     1741 aagcgaatgc tggcgcgcgt tacaccatta tgcgtccagt cagtattgag gcgggttatc
     1801 gctacctgaa tctgtcgggt aaagacggta accgcgacaa cgccgtggct gacggcccgt
     1861 atgttggggt taacgccagt ttctgatccc ttccggcgcg gcattctgtc gcgccattca
     1921 gtttcttact acgccttatc tccctttgcg ttatagtgat tcgataacaa aagcaggaga
     1981 taaccatgtt aaaagtggaa atgctatcca ccggggatga agtgttacac gggcaaatcg
     2041 ttgacactaa cgctgcctgg ctggccgatt ttttctttca tcaggggttg ccattatctc
     2101 gccgcaatac ggtgggggat aaccttgatg acttagtcac cattcttcgc gaacgtagtc
     2161 agcacgccga tgtgctgatc gttaacggcg ggctgggacc gaccagcgat gatttaagcg
     2221 cactcgccgc tgcgacagca aaaggtgaag gcctggtgct gcatgaagcc tggctcaaag
     2281 agatggaacg ctatttccac gaacgtggac gagtaatggc accgagcaac cgtaaacaag
     2341 cggagctgcc tgccagtgct gaatttatca ataacccggt aggcaccgcc tgtggttttg
     2401 ccgtgcagct taatcgttgc ctgatgttct ttactcccgg cgtaccgtca gaatttaagg
     2461 tgatggtcga gcacgaaatc ctgccgcgtc tgcgcgagcg tttttcttta ccacagccgc
     2521 cggtttgtct gcgtttaact acctttggtc gttcggaaag cgatctggca caaagcctgg
     2581 acactctaca actgccgccg ggcgtaacaa tgggctatcg ctcctcaatg ccgatcatcg
     2641 aactgaaact caccggaccg gcaagcgagc aacaggcgat ggaaaaactg tggctggacg
     2701 ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac cgaaggactg cccgcgcaga
     2761 tcagtcgcga attgcaaaac cgccagttca gcctgacgtt gagcgagcaa ttcaccggtg
     2821 gtttattggc tttgcaactt tctcgcgcag gtgctccact gctggcgtgt gaagtggttc
     2881 cttcacagga agaaaccctg gcgcaaactg cgcactggat tacagaacgg cgagccaacc
     2941 attttgccgg gctggcactg gctgtttcgg gtttcgagaa cgagcatctc aactttgcgc
     3001 tagccacgcc agatggcact ttagctctgc gtgtgcgttt cagcactaca cgctatagcc
     3061 tggctatccg tcaggaagtg tgcgcaatga tggcactgaa tatgctacgc cgttggttaa
     3121 acggccagga cattgccagt gagcatggct ggattgaggt tgttgagtcc atgaccttat
     3181 ccgtctgatt ccgccactct gtgccggttt actgcaccgg cacattcccc ctccggcaaa
     3241 acttgtccaa cctgttgtac tcaataagaa aaaatccgtg cgctgattca caaatttgtg
     3301 cgtaatttct ccagtccgtt ggacgaggag cgggatttga tgcgcttcac aattttactc
     3361 cccctgagca acgagactga ttccagtatc taagactgga gtccagtatg ttggaatcaa
     3421 gcaaagttcc cgccctgaca cgggctattg atattctcaa tttgattgcc cgcattggcc
     3481 cctgtaatgc tgcgacgatc attgacacat tggggatccc taaaagcacg gcctatttgc
     3541 tgcttaatga actcaggcgt cagcgttttc ttagccttga tcaccaggaa aatttttgtt
     3601 tgtggaccaa gctggtggag ctttccggtc atgcgttaag caagatggat ctccgggaac
     3661 tggctcgacc gcgtttgacg caactgatgg ataccaccgg gctgttatgt cacctgggga
     3721 ttattgataa cggcagcgct tattacattt tgaaagtgga gtcatcagcc actatcagcg
     3781 tgcgttcaca tgaaggaaaa agcctttcgc tttatcgctc cggaataggt aaatgcctgc
     3841 ttgcctggca acctgcaact gtacagcaaa gtattatcga agggctggtc tgggagcagg
     3901 cgacacccac aacgattact catccacagc aacttcacga agaactggcg cgtattcgcc
     3961 gtcagggctg gagctatgac aacggcgaag attacgccga tgtccgttgt gtcgctgcgc
     4021 cggtgtttaa cgccaataac gagcttaccg ctgcgatctc ggtggtcggt acccgcttac
     4081 aaatcaatga agaataccgt gattatctgg cgggcaaggc cattgcctgc gccagggata
     4141 tttcacgtct gttgggatgg aaaagtccct ttgacttaca agcctcataa caatggagaa
     4201 catcatgacc ctaccaaaaa ttaaacaggt tcgcgcctgg tttactggcg gtgcgacagc
     4261 agaaaaaggc gctggcggcg gtgattatca cgaccagggg gcgaatcact ggatagacga
     4321 tcatattgcc accccgatga gtaaataccg cgattacgag cagttacgcc agtcatttgg
     4381 cattaacgtt cttggcacgt tggttgttga agtcgaagca gaaaacggcc agaccggatt
     4441 cgctgtttcg acagccggtg aaatgggctg ttttatcgtc gaaaaacacc ttaaccgttt
     4501 cattgagggt aaatgtgtca gtgatatcaa actgatccat gatcaaatgc tcaatgccac
     4561 cctgtattac tccggctctg gtggcctggt gatgaacacg atttcctgtg tcgatctggc
     4621 tctgtgggat ctgttcggca aagtggtcgg gcttccggtt tataaacttt taggcggcgc
     4681 tgttcgtgat gagattcagt tctacgccac aggtgcgcgt ccggatctgg caaaagagat
     4741 gggctttatc ggtggcaaaa tgccgacgca ctgggggcca catgatggcg atgcggggat
     4801 ccgcaaagat gccgctatgg tcgcggatat gcgtgaaaaa tgcggtgagg atttctggtt
     4861 aatgctcgac tgctggatga gtcaggacgt gaactatgcg accaaactgg cccacgcttg
     4921 cgcgccctat aacctgaaat ggatcgaaga gtgcctgccg ccacagcagt atgaaggtta
     4981 tcgcgaactg aaacgcaacg cgccagtcgg aatgatggtc accagcggtg agcaccacgg
     5041 cacactgcaa tcttttcgta cgctttcaga aaccggtatc gacattatgc agccggatgt
     5101 tggctggtgc ggtggcttaa ccacgctggt ggaaattgcc gcaatcgcca aatccagggg
     5161 gcaactggtg gtgccgcacg gttcgtctgt ttactcccac catgcggtga tcaccttcac
     5221 taatacgcca ttcagcgaat tcctgatgac tagtccggat tgttcaacga tgcgtccgca
     5281 gtttgacccg attctgctca atgagccggt tccggtgaat ggtcgtattc ataaatcagt
     5341 gcttgataaa cccggttttg gcgtcgaact caatcgtgac tgcaatttga aacgccccta
     5401 cagccactaa tcactccggg cgttgctgtg acaacggtaa cgcccacata ttctaaggag
     5461 ctttttatga gcaccgcttt gcttgacgcc gtggtgaaga aaaaccgtgt gcgtttaatt
     5521 ccgtttatgt tggcgctgta tgtgctggcg tttctcgacc gttcgaatat cggttttgcc
     5581 aaacagacct accagattga taccgggttg agtaatgaag cttatgcgct gggagcaggc
     5641 attttctttg tggtatatgc gtttctgggt gttccggcga atcttttgat gcgcaaactg
     5701 ggggccagaa cctggattgg tacgacaaca ctgctgtggg gatttctttc ggcagccatg
     5761 gcatgggccg atactgaagc gaaatttctg atagttcgca ctctgcttgg tgctgcggag
     5821 gccggatttt tccctggtat gatttatctc acctcgcaat ggtttccgca gcgtaatcgc
     5881 gccagcatta tggggctgtt ctatatgggc gcaccgctgg cgttaacact gggatcaccg
     5941 ctttccggcg cgctgctgga gatgcatgga tttatggggc atcccggctg gttctggatg
     6001 tttgtgattg aaggattgtt ggcagtcggc gctggggtat tcacattctt ttggcttgat
     6061 gacacaccgg agcaggcacg ttttctgagt aaactagaaa aaacgttgct tatcaatcaa
     6121 ctggcaagtg aagaacaaca gaaagtgact tctcggctga gcgatgcgct gcgtaatggg
     6181 cgagtctggc aactggcgat tatctacctg accattcagg tggcggttta cggattaatt
     6241 ttcttcctgc cgacccaggt tgcggcattg ctgggaacaa aagtgggctt tacagcgtcg
     6301 gtggtcaccg ccattccgtg ggttgcggcc ttgtttggga cctggcttat tccgcgctat
     6361 tccgataaaa ccggcgaacg gcgtaatgtc gcagcgctga cattactggc ggcgggcatt
     6421 ggtattggtc tgtccgggct gctttctcca gtactggcga tcgtagcgct gtgtgttgca
     6481 gccatcgggt ttattgccgt gcagccggtg ttctggacga tgccgacaca actgctttcc
     6541 ggtacggcgc tggctgcggg gattggtttt gtaaacctgc ttggtgcagt gggcgggttt
     6601 attgccccga tcctgcgcgt gaaagcagaa acgttatttg ccagcgatgc ggcgggatta
     6661 ctgacgctgg cagcggtggc ggtcatcggt tcgctgatta ttttcactct gcgtgtaaat
     6721 cgcaccgttg cgcagaccga cgtggcacat cattaaatag gttaaggaac acgatgaacg
     6781 cattattaac catcccttta aagaacgttt acgcaagggc gaagtgcaaa ttggtctgtg
     6841 gttaagctca acgactgcct atatggcaga aattgccgct acttccggtt atgactggtt
     6901 gctgattgac ggggagcatg cgccaaacac cattcaggat ctttatcacc agctacaggc
     6961 ggtagccccc tatgccagcc aacccgtgat ccgtccggtg gaaggcagta aaccgctgat
     7021 taaacaagtc ctggatattg gcgcgcaaac tctactgatc ccgatggtcg atactgccga
     7081 tcaggcacgt caggtggtgt ctgccacgcg ctatcctccc tacggtgaac gtggtgttgg
     7141 ggccagtgta gcacgggctg cgcgctgggg acgcattgag aattacatgg cgcaagtcaa
     7201 cgattcgctt tgtctgttag tgcaggtgga aagtaaaacg gcactggata acctggacga
     7261 gatcctcgac gtcgaaggga ttgatggcgt gtttattgga cctgcggatc tttctgcgtc
     7321 gttgggctac ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat
     7381 tcggcggatc cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc
     7441 gcagcaatgc ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta
     7501 cagcgatgcc ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat
     7561 aaaaggtagt tattgatatc aaaggcccat ggggatcggc tgtgggactg tgttaattag
     7621 tggttattcg ctgccagatc ggcttcgctt agctgggtgg ccgcgagcac caggtcacga
     7681 tcaaagccct gttccagcat gcgcaaggca attttaatag cttgttcatg catgccttcc
     7741 tgcaaacctt ctaatttacc ttcctgccaa ccaatttgat gtccttcctg ccgtagccgt
     7801 tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat ctcctgaata
     7861 aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag tgtttgtagc
     7921 tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac cataccgatt
     7981 aaatcgcggt cgcgaatatg cttttgaatc agttcaagca gagctatacg ccgatgttgc
     8041 atgatctcat cgtcaggtac gatggtaata tccaccaacg gaaaagcttc ggtgtacaac
     8101 tgccgggcaa gttgcggatc gtcaaactca tccagccagt tgagtgagta cgggtagggc
     8161 gaggtttcgc catgataaaa cagcaacggc accaccagcg ggactctgtc atagcctttg
     8221 tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc catattcttt
     8281 tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc gctggtttcc
     8341 acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa actagtgggt
     8401 tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg gttctggtaa atgtatttcg
     8461 agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaatactgc atcatgcggg
     8521 gaggaggttg ttgattcggt catggttgtc cgtcacctga tatttatgat gacgcgacat
     8581 tacggttatc ccaggccttt gacatctgcc agtttcagcc tttgcgagac gttttcagag
     8641 gaattttcac tgcgttgcaa cgcatacatt gggcacggaa tcgtgtacgc aaaaatgccc
     8701 ggtaaccaca tgcttaccgg gcatcagaaa ggagtttaag ccagcgcctg ggccagtagc
     8761 gtaatcggat gttcgcagcg aagacttgtg gacatctcaa tctgccattt acaggtttcg
     8821 cagtcggtga ccaccagatc tgcgccgctt tcttctatct ggcggaacag tggtgcgccg
     8881 atggcttgtg aggtggggta gttctctttt ttgaaaccgt aagtacccgc aataccgcag
     8941 cactgggaat ccagcaccgt taactcaagc cccgggatgt tacgcaacag ctccagggtg
     9001 tagagcgtcc agcccatttt ttccatatgg cacggagtgt gataaaccac tttcagcggc
     9061 agcggtttca gcggtaacgt tttgccttcg tccagcttgc gccacagcca gcgggtcgcc
     9121 agttcgatat gatcgcgcaa gcctttgttg tcgacattca gcacttccgg gtattcgtcg
     9181 cgcagggcaa atgtacaggt tgaggaggtg gcaatcaccg gaatgccttt tactcccaca
     9241 gcttcgcgga tcgactctac attcgtaatt gcctgtttgc gtgctttatc ggtaaagccg
     9301 ttggcgatca gcggtacgcc gcagcatttt tctttgctga gcagttgtac accggtaccc
     9361 attgcgttga gcactttaat taaatcttta cctaactgcg gatggttgta gttaacgaag
     9421 cagccgtgaa agaaagcgac ctggtcttta tactgcgctt gctgagccgc cacgctgcga
     9481 taccagcgac gaaacgtgcc gaaggagtat ttcggtagcg tgcggcgatg atcgattttt
     9541 aacgccgcat caagcaactg ccgcaccggt ttcagcgagg tagcggtgtt gacgattggt
     9601 gcgaacggcg tggaaacgct acccatcagg tcggtatggc ttaaaacaaa attgcgcagc
     9661 gacgggcgcg tggtgtcata tttcgcccgt gcgcgctgga tgatatcgcc aatcttcaca
     9721 tcggacgggc aggcgacttc gcaacgtttg cagttgatgc aatatttcag tgcctcgtca
     9781 tacagtgcgc catcttttaa acgcagacgc tcgccatccg gcccggcttg ttttggccct
     9841 ggataaccgg gattcacccg gctcactggg caggcggtgg tgcagacggt gcacttaatg
     9901 cagttttcga agctggtgtc attcattgtt ggcctcctgc gcgttgggca atctgttgag
     9961 cggcatgtaa agcactgacg gcacaaacac cgccgccgca tccctgggcg atgggatcaa
    10021 atccgcccag caccgaaccg atggtaaaca ggttttcgat ggtttgccct gcctgcgacg
    10081 ggcgtagcgt ctgatcagtg gttacaccga actgctgcca cggttgcggc gcaaaaaaat
    10141 ctcccttata ccattcaccc cgcgtggcgg tttgtagcac atcaaggccg agaatcggct
    10201 ctcgaatgcc gttacgttcc gctaccagtc cgccactaaa gaaactgccg ctggcgagaa
    10261 ccgcgaaacg tggacgcagc gggatatcgg cgtgattgcg ggtccagatt tcattcacta
    10321 cgccattttt acaggtcact tttttcactt catcgcccgg catccacacg ccaccctgac
    10381 gcacaaactg gcgctgtaac tggttttgca gacgaatgcc cagcacggaa ggcggcagcg
    10441 ttggcaaaag catcagtgaa caaggtagtt tttcattcaa ccaacgccac agtttgtcat
    10501 cagccaggcc gaagcaggcg ggcatcagga tcatttcgca ggtattggcg acaggaataa
    10561 gcgcatcaag taacagcggc cagttttctt cattatcaag gaaacgggcg atattcaccg
    10621 cgcggaactc ggtggcgtta ttgcgcagga catccagttc cggcagctct atttctgcgg
    10681 tttcaacggc aaggtcgagt tcacgcaacg aagctgccgc aaggtgcgcc tgaaaatcca
    10741 tcagtccgct aattcccact acacatattt tcttcgcggg cagcggccag acggggactt
    10801 ctggcgaact tagccaggta gagcgcagag tgccgagcgg cgtaatccgc tggtgaggca
    10861 gttctacgct gccctgcaat tgcgcaccgc tttcagcgat taatgcctgc gcctggcaag
    10921 cgagatcgag cacgcgttgt ggcccgagaa gagagtaagg atgtgcaggt gcctgctgac
    10981 gcaaagattc cagtccactg tgaatgtctg tcacagcttg accatctggc agatggctca
    11041 gcaaatccag cgaaccggat gagaaatgca gcgcgctttg accacgcgtg acaatggcac
    11101 agcgcaggcc gtgtttttgc agctgcaggc cacagagtaa tccggcgagg ccgccgccca
    11161 taatgatagt atcaaagcgc atctttctgc tccttctcca gaccacacag tccctgataa
    11221 acccagcggg taaattcgct ttcgcgcagt gcatctcccc aggcgatggg ttgcacgcct
    11281 ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga cgtcgtgacg
    11341 ttaaaacgtt gcagcagtcc agcagcgcgg caggcgcaga gttcgccctg gcaggtaccc
    11401 atccccacac gggtacgacg gcgtaaatcc agcaggctat taacgtttaa attttctacc
    11461 gcgtactgca cttcacccgc agttaccgct tcgcactcac ataccaggct acggtgcaga
    11521 cggccttcgc tcagccaggc aggcgtgcga tcgccatgac gataaaccgc agaaccgcgc
    11581 agcggggcag gcagggagat gactttacgc aaggtaactt cagcgggttc ctgtgaaccc
    11641 ggaagtgcca gatcggcggt cgtacagggg cgcgtgttgc ccagtttgcg gcataccgcg
    11701 tcggtagccc attcagccat cagccgatag gtcatcagtt tgccaccggt gatggtgata
    11761 aatccgtcca gaccatcgcg ttcagcatgg tcgagcagca cgatgccacg gctgacgtta
    11821 cgtccgctcg ggtcgtcatc gctggcaacc agcgggcgca cgccagaata ggcccgcaaa
    11881 atgcgcgttt tcgccatcac gggggccagt ttttcccctt cacgcagcag aatatcaacc
    11941 tcttctgccg tcactcgatt atcgtcaatc tcgttgtaat caatacgtaa agaggtggtg
    12001 ccaatcagcg aaatggtatc gccaggcacc agaatatcgg cgtcggaagg tttacggcag
    12061 cggttgatca catgctgatt aatgcggtga tccatgatca gcagcgatcc tttcgccggg
    12121 aacatgcgaa tgcgcagatc ggcatattcg gcaatgtgtt gcccccagat cccagcggca
    12181 ttaaccacga caggtgcatg aagggcctga gtttcgccgg tgagatgatt acgtacacga
    12241 acaccgcaca ccgtcgcgcc ttcacgaatc agccccgtga cttcatgagc ggtaaggata
    12301 acagcaccgt gttctttggc atccagcatg tttgctgcgg tcagacgaaa tggatcaacg
    12361 gtgccatccg gaactttcac cgcgccaatc agtgccgggt taacggcagg ttcgataatg
    12421 cgcgcttgct gcgggtctat agcttctgcg ctgatccctg cttcttcgca ggcgcgaata
    12481 aaagtggcct ggaaggagag gtcatcttcc ggcagggtga taaacaggcc gttggttggt
    12541 tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt cactaatgca ttcgcgggcc
    12601 gattccgcat cggttaccgc atagcgcgca ccgctgtgca gcaggccgtg gttacgcccg
    12661 gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc gcgcagggca
    12721 cagtcgcggg caattccggc tcccgttgcg ccgccgccaa tgataatcac gtcacttgat
    12781 tgcgagtcgc gagttttcat tgttatccct ctgaagttcg ttttttacca tttagccata
    12841 gtaaaaacat gagttgtttg atttcgcgca tattcgctca taattcgaaa gtgagacgtg
    12901 atttcatgcg tcattttgaa cattttgtaa atcttattta ataatgtgtg cggcaattca
    12961 catttaattt atgaatgttt tcttaacatc gcggcaactc aagaaacggc aggttctctc
    13021 actgaatcag gctgttaatc ataaataaga ccacgggcca cggaggcttt caatgttgag
    13081 tatttttaaa ccagcgccac acaaagcgcg cttacctgcc gcggagatcg atccgactta
    13141 tcgtcgattg cgctggcaaa ttttcctggg gatattcttt ggctatgctg cttactattt
    13201 ggttcgtaag aactttgcgc tcgctatgcc ttacctggtg gagcagggat tctcacgcgg
    13261 tgatttaggt tttgcgcttt ctgggatctc gatcgcctat ggattttcga aattcatcat
    13321 gggttcggta tcggatcgct cgaatccgcg cgttttcctg cccgcaggtt tgattctggc
    13381 ggcggcagtg atgttgttta tgggctttgt gccatgggcg acgtcgagca ttgcggtgat
    13441 gtttgtactg ttgttcctct gcggttggtt ccaggggatg gggtggccgc catgcggtcg
    13501 tactatggtg cactggtggt cgcagaaaga acgtggcggc attgtgtcag tgtggaactg
    13561 tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc ctgctgggga tggcctggtt
    13621 caatgactgg cacgcggcgc tctatatgcc cgctttctgc gccattctgg tggcattatt
    13681 cgcctttgcg atgatgcgcg ataccccgca atcctgtggc ttgccgccga tcgaagagta
    13741 caaaaatgat tatccggacg actataacga aaaagcggaa caggagctga ccgcgaagca
    13801 aatcttcatg cagtacgtgc tgccgaacaa actgttgtgg tatatcgcca tcgccaacgt
    13861 gttcgtttat ctgctgcgtt acggcatcct cgactggtca ccgacttatc tgaaagaggt
    13921 taagcatttc gcgctggata aatcctcctg ggcctacttc ttctatgagt atgcgggtat
    13981 tccgggcacc ctgctgtgcg gctggatgtc ggataaagtc ttccgtggca accgtggggc
    14041 aaccggcgtt ttctttatga cactggtgac catcgcgact atcgtttact ggatgaaccc
    14101 ggcaggtaac ccaaccgtcg atatgatttg tatgattgtt atcggcttcc tgatctacgg
    14161 ccctgtgatg ctgatcggtc tgcatgcgct ggaactggca ccgaaaaaag cggcaggaac
    14221 ggcagcgggc tttaccggtc tgtttggtta cctgggcggt tcggtggcgg cgagcgcgat
    14281 tgttggctac accgtggact tcttcggctg ggatggcggc tttatggtaa tgattggcgg
    14341 cagcattcta gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatca
    14401 ggaattactg caaaaacgca acggaggcta atggcatgaa attgacgctg aaaaacctta
    14461 gcatggcgat catgatgagc actatagtca tgggaagcag tgcaatggcg gcggacagca
    14521 acgaaaaaat agtcatcgcc catcgcggtg ccagtggata tttgccggag catacgctgc
    14581 cagcaaaagc gatggcgtat gcgcagggag cggattatct ggaacaggat ttggtgatga
    14641 ccaaagacga ccatctggtt gttctgcatg accattacct cgatcgtgtt actgatgttg
    14701 ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata gatttcacgc
    14761 tggatgaaat taagtcgttg aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag
    14821 tgcagactta tccagggcgt ttcccaatgg gtaagtccga cttccgggtg cacacctttg
    14881 aagaagagat tgaatttgtt caggggttaa atcactctac cgggaaaaat atcggtatct
    14941 atccagaaat caaagcgccg tggttccatc atcaggaagg gaaggatatt gcggcaaaaa
    15001 cgctggaagt gctgaagaaa tatggttaca ccggtaaaga cgataaagtt tatttgcaat
    15061 gttttgatgc tgatgagctg aagcgtatta agaatgagct ggaacccaaa atgggcatgg
    15121 atctcaatct ggtacagctg attgcctata ccgactggaa tgaaacgcag cagaaacagc
    15181 cggacggaag ctgggttaat tacaactacg actggatgtt taagccgggt gccatgaaac
    15241 aggtggcgga atatgcagat ggtattggtc cggattacca tatgttgatt gaggagacat
    15301 cgcagccggg taatatcaaa ctcactggca tggtgcaaga tgctcagcag aacaagctgg
    15361 tagtgcatcc ttataccgtg cggtcagata aactgcctga atacacaact gatgtgaatc
    15421 agttatatga tgctctgtat aacaaagcgg gtgtaaatgg gttgtttact gatttccctg
    15481 ataaagcggt taaattcctt aataaagagt aatgctgcta ctgcaggatg gcgttcatgc
    15541 gccatcctct gctaaagcga aataatgcta ctaccggtta ttcttttcat taaccggtgc
    15601 gtcctgtaaa aaagatgcca acataatttt cgtaaaactc attcgttaaa aaagctaaca
    15661 ctgg
//
LOCUS       DBFIFS010000099        15612 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.099, whole genome shotgun sequence.
ACCESSION   DBFIFS010000099 DBFIFS010000000
VERSION     DBFIFS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.099"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            60..578
                     /gene="yfiR"
                     /locus_tag="N7A07_002737"
     CDS             60..578
                     /gene="yfiR"
                     /locus_tag="N7A07_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU2477373.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            568..1794
                     /gene="dgcN"
                     /locus_tag="N7A07_002738"
     CDS             568..1794
                     /gene="dgcN"
                     /locus_tag="N7A07_002738"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU2477374.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1810..2292
                     /gene="yfiB"
                     /locus_tag="N7A07_002739"
     CDS             1810..2292
                     /gene="yfiB"
                     /locus_tag="N7A07_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU2477375.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(2369..2716)
                     /gene="rplS"
                     /locus_tag="N7A07_002740"
     CDS             complement(2369..2716)
                     /gene="rplS"
                     /locus_tag="N7A07_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU2477376.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2758..3525)
                     /gene="trmD"
                     /locus_tag="N7A07_002741"
     CDS             complement(2758..3525)
                     /gene="trmD"
                     /locus_tag="N7A07_002741"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU2477377.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3556..4104)
                     /gene="rimM"
                     /locus_tag="N7A07_002742"
     CDS             complement(3556..4104)
                     /gene="rimM"
                     /locus_tag="N7A07_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU2477378.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4123..4371)
                     /gene="rpsP"
                     /locus_tag="N7A07_002743"
     CDS             complement(4123..4371)
                     /gene="rpsP"
                     /locus_tag="N7A07_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU2477379.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4508..5869)
                     /gene="ffh"
                     /locus_tag="N7A07_002744"
     CDS             complement(4508..5869)
                     /gene="ffh"
                     /locus_tag="N7A07_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU2477380.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6036..6827
                     /gene="ypjD"
                     /locus_tag="N7A07_002745"
     CDS             6036..6827
                     /gene="ypjD"
                     /locus_tag="N7A07_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU2477381.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6849..8134
                     /gene="yfjD"
                     /locus_tag="N7A07_002746"
                     /pseudo
     CDS             6849..8134
                     /gene="yfjD"
                     /locus_tag="N7A07_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            complement(8189..8782)
                     /gene="grpE"
                     /locus_tag="N7A07_002747"
     CDS             complement(8189..8782)
                     /gene="grpE"
                     /locus_tag="N7A07_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU2477382.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKVKA"
     gene            8905..9783
                     /gene="nadK"
                     /locus_tag="N7A07_002748"
     CDS             8905..9783
                     /gene="nadK"
                     /locus_tag="N7A07_002748"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU2477383.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9869..11530
                     /gene="recN"
                     /locus_tag="N7A07_002749"
     CDS             9869..11530
                     /gene="recN"
                     /locus_tag="N7A07_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU2477384.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARAVLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11679..12020
                     /gene="bamE"
                     /locus_tag="N7A07_002750"
     CDS             11679..12020
                     /gene="bamE"
                     /locus_tag="N7A07_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU2477385.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12082..12372)
                     /gene="yfjF"
                     /locus_tag="N7A07_002751"
     CDS             complement(12082..12372)
                     /gene="yfjF"
                     /locus_tag="N7A07_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU2477386.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12362..12799)
                     /gene="ratA"
                     /locus_tag="N7A07_002752"
     CDS             complement(12362..12799)
                     /gene="ratA"
                     /locus_tag="N7A07_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU2477387.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            12970..13452
                     /gene="smpB"
                     /locus_tag="N7A07_002753"
     CDS             12970..13452
                     /gene="smpB"
                     /locus_tag="N7A07_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU2477388.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13667..14029
                     /gene="ssrA"
                     /locus_tag="N7A07_002754"
     tmRNA           13667..14029
                     /gene="ssrA"
                     /locus_tag="N7A07_002754"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14298..14546
                     /locus_tag="N7A07_002755"
     CDS             14298..14546
                     /locus_tag="N7A07_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2477389.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(14662..14814)
                     /locus_tag="N7A07_002756"
                     /pseudo
     CDS             complement(14662..14814)
                     /locus_tag="N7A07_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(15048..>15612)
                     /gene="espM2"
                     /locus_tag="N7A07_002757"
     CDS             complement(15048..>15612)
                     /gene="espM2"
                     /locus_tag="N7A07_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HGU2477390.1"
                     /translation="FSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTISFSVRKFSG
                     NILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEERQRVFFLVS
                     QENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTIANLLADKFY
                     QKHIDSDIDIVKLRNDIPDYLMRAIQG"
BASE COUNT         3971 a         4080 c         3617 g         3944 t
ORIGIN      
        1 taatgagcat tctgaaaaaa tatcgatgca tttcgagcga agatggtggg gatccctgaa
       61 tgcgtttttc tcaccgactg ttcttactgc tgattctcct gctgacggga gcaccaatac
      121 tcgctcagga gccttctgac gttgcgaaaa acgtgcgtat gatggtttcc ggtattgtca
      181 gttacacccg ctggcctgcg ctatcagggc caccaaagtt atgtatcttt tcatcttcac
      241 gttttagtac cgccctacaa gagaatgccg caacatcttt gccttatctg cctgtcatca
      301 ttcacactca acaagaggcg atgatttcag gctgcaatgg tttttatttt ggcaacgagt
      361 caccgacatt ccagatggaa ttaaccgaac aatatccgtc aaaggcgtta ttattaattg
      421 ccgaacaaaa taccgagtgc attattggta gcgccttttg tctgatcatc cacaataatg
      481 acgtcagatt tgccgtaaac ctggatgcct tatcgcgtag cggtgtaaag gtcaacccgg
      541 atgtcttaat gctcgcacgg aagaaaaatg atggataacg ataattctct taataagcgc
      601 cccacgttta aaagagcatt acgcaacatc agtatgacca gcatatttat cactatgatg
      661 ctgatctggt tgctgctttc cgtgacctcg gtgctgcccc tgaaacagta cgcgcaaaaa
      721 aacctggcac tgacagcagc aacaatgact tacagtctgg aagcagctgt cgtttttgcc
      781 gatggccctg cagcaactga aacactggca gcgctgggcc agcaagggca attttcaact
      841 gcagaagtac gtgataagca gcaaaatatt ctggcatcct ggcattacac ccgtaaggat
      901 ccaggcgata ctttcagcaa tttcataagc cactggctct tccctgcccc catcattcag
      961 ccgattcgtc acaatggtga aaccattggc gaagtacgct taaccgctcg cgacagttca
     1021 atcagccatt ttatctggtt ttcgctcgcc gtactgaccg gttgtattct gctggcatca
     1081 ggaatcgcaa ttaccctcac ccgccatttg cacaatggcc tggtggaagc actgaaaaat
     1141 atcaccgatg tcgtacatga tgtgcgttcc aaccgcaatt tttcccgacg agtttcggaa
     1201 gaacgtatcg ctgagtttca ccgcttcgct ctcgacttca acagtctgct ggatgaaatg
     1261 gaagagtggc agcttcgttt acaggccaaa aatgcgcagc ttctacgtac cgcgctacat
     1321 gacccattaa ccgggctggc taaccgcgca gcgtttcgta gcggcatcaa tacgttgatg
     1381 aacaattccg ctgcccgaaa aacgtcggcg ttactatttc ttgatggcga taatttcaaa
     1441 tatatcaatg atacctgggg tcatgcgacg ggcgatagag tcttgattga aatcgcaaaa
     1501 cggttagctg aatttggcgg gctgcgacat aaagcatacc gcctgggcgg cgatgaattc
     1561 gctatggtgc tctatggtgt acagtcggaa tctgaagtgc agcagatatg ctcagcactg
     1621 acacaaatct ttaatctccc gtttgatctt cataatggcc atcagaccac catgacatta
     1681 agcattggtt acgcaatgac cattgcgcac gcttctgcgg aaaaattaca agagctggcc
     1741 gatcacaata tgtatcaggc caaacaccag cgtgccgaaa agctggtgag ataacaagga
     1801 tatatcgata tgataaagca cctggtagca cccctgattt tcacctcact aatactgact
     1861 ggctgccagt cccctcaggg aaagtttact cctgagcaag tcgccgctat gcaatcttat
     1921 ggatttactg aatccgccgg cgactggtcg ctgggcttat cagatgccat tctgttcgca
     1981 aaaaatgact acaaattgct cccggaaagc cagcaacaga tccaaaccat ggcagccaaa
     2041 ttggcctcga cagggctaac acatgcccgt atggatggac acaccgataa ctatggtgaa
     2101 gacagttaca acgaaggctt atcattgaaa cgggcgaatg tcgtggccga tgcatgggct
     2161 atgggtggac aaattccacg cagcaatctc accacacagg gtttaggaaa aaaatatccc
     2221 atagccagta acaagaccgc ccagggccgc gccgagaacc gccgcgtcgc agtggtgatt
     2281 actccccctt aaaacacgag caaacagata aaaaaagggc cagccaattg gccagccctt
     2341 cttaacagga tgtcgcttaa gcgaaatctt agttaagacg ctctttgata cgagcagcct
     2401 taccagtacg ctcacgcagg tagtacagtt tagctttacg aacagcacca cgacgtttga
     2461 cagaaatgct gtcaactacc ggagagtgag tctggaagac acgctcaacg ccttcgccgt
     2521 tggaaatttt acgaacagtg aatgcagagt gcagaccgcg gttacgaata gcgataacca
     2581 cgccctcgaa tgcctgcaga cgttttttgg aaccttcaac aacccatact ttcacttcca
     2641 cggtatcacc cggacggaag gaaggtacgt cctgcttcat ctgctcttgt tcaagttgct
     2701 taataatgtt gctcataatt taatctctta tcctgggtaa actgatatct cgggggctta
     2761 cgccatccca tcatgtttat gttgctgttg tgcgtgttcc gttttgaact ccgccagcaa
     2821 ccttgcttgc tcttcagtca gagccaggtt ttccagaagt tcaggtcttc taagccaggt
     2881 acggcccagc gactgtttca aacgccagcg acgtatctcg gcatggttgc ccgacagtaa
     2941 cactggcgga acttccatcc cttctaacac ctcaggccgc gtatagtgcg ggcaatccag
     3001 caatccttca gcaaaggaat cttccgttgc cgaggcttca tgtcccagta ctcccggaat
     3061 aaaccgggaa acggagtcaa tcagcgtcat tgctggtaac tcaccaccac tgagaacgta
     3121 atcgccgatt gaccattctt cgtcaatttc ggtttggatc acgcgctcat ctataccttc
     3181 gtagcgaccg cacaccagaa tcaatttttg attcgttgcc agttcgctga cgcccgcttg
     3241 atcaagcttg cgtccctgtg gtgaaagata aatcaccttt gcgccttcac ccgccgcggc
     3301 ttttgctgca tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc ccggtccgcc
     3361 gccgtaagga cgatcgtcca cggtacggtg ccggtcatgc gtgaaatcgc gaggactcca
     3421 gctctggatg ctcagcaggc catttttaac tgcccggcca gttaccccgt aatcggtaat
     3481 tgcgcggaac atttcaggaa acaggctaat tatgccaatc cacatagcgc cgtcttttac
     3541 cgtttatccg gtggtttaaa aaccaggatc ccaatctact tcgattgaac gagtagtgag
     3601 atcgactttc ttgataacct gcccatcgag gaacggtacg agacgttcct tgataccaaa
     3661 cgcatctttc aggtttgcct taatgacgag aacgtcattg gatccggttt ccatcatatc
     3721 gacgacttta ccgagatcgt agccttcagt ggttactacc tggcagccca tcaggtcttt
     3781 ccagtagtag tcaccctctt caagctgagg cagctgcgat gaatccacga caatttcaca
     3841 attcgtcagc aggttcgccg catcacgatc gtcaacgcct ttcagcttga tgatcatgtc
     3901 ctgattgtgg tgcttccagc tttccagctg gacttgctgc cactgacccg ccttctggat
     3961 aaaccagggc tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa acactctgag
     4021 ccacccacga ataccgtaag acgaacccat ttttcccaaa acgatgggat caacaggtgc
     4081 ttgcgcggtg agttgtttgc tcatcatgac caccgtgaca gattaagctg ctttgtttac
     4141 ttctttgatc agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa cccagtgagc
     4201 gatgcgatcc agatccaggc gagtgccttc ttctttttcg ctagcgattg ggttgaagaa
     4261 accaacgcgc tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga caacaacctg
     4321 gtagaacgga cgctttttag cgccgtgacg tgctaaacga atagttacca taacatcctc
     4381 ttgtgtgaat aaaacaaccg gaccccatcg aggaacggag tccggtgtca tattaaaagc
     4441 ccgaaaattt tactgatttc tggagaaaat gcaatcagca gttaataact ctgtcgcata
     4501 aggtcattta gcgaccagga aatcccggcg gcatcatccc cttcatgctt ctcatcatct
     4561 tcgccattcc gcccttcttc attttcttca tcatgcgctg catgtcgtcg aactgtttca
     4621 gaagacggtt aacgtcctgc acctgcatac cgcaaccggc agcaatacgg cgtttacgcg
     4681 aacctttgat gatttctggc ttagcgcgct ctttcatcgt catcgagttg atgatggctt
     4741 ccatacgcac cagcacttta tcgtccatct gtgacttgac gttatccggg atctgcccca
     4801 tgcccggcag tttgcccatc aggctggcca tgccgcccat atttttcatc tggcgcagtt
     4861 gctcaaggaa gtcgttgaga tcgaagccgt cacctttttt cagcttgctg gctaatttct
     4921 ctgcctgcgc gcggtcaact ttgctttcga tatcttcgat cagcgacagt acgtcgccca
     4981 tgccgagaat acgcgacgcg atgcggtccg gatggaacgg ctccagcgcc tcagttttct
     5041 cgccaacacc gaggaacttg atcggtttgc cagtgatgtg acgaatagag agcgccgcac
     5101 cgccgcgggc atcgccgtcc actttggtca acactacgcc ggtaagcggt aacgcttcat
     5161 tgaatgcttt tgccgtattg gccgcatcct gaccggtcat ggcgtcaacc acaaacaggg
     5221 tttcaaccgg gttaatcgac gcatggactt gtttgatctc gtccatcatc gcttcgtcaa
     5281 cgtgcagacg accagcggta tccaccagca gcacgtcgta gaatttcagt ttggcttctt
     5341 tcagcgccgc gttaacaata tcaaccggct tctgaccaac atcagaaggg aagaaatcaa
     5401 cgcccacctg ctctgccagc gtctcaagct gtttgattgc cgccgggcga tagacgtcag
     5461 cagaaaccac cagcactttc ttcttgtgct tctcgcgcag gaacttaccg agtttaccaa
     5521 cgctggttgt tttaccggca ccttgcaggc ctgccatcag cacgaccgca ggcggttgcg
     5581 cagccagatt cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg ttacggacga
     5641 ttttgacgaa ctcctgcccc ggcgtcaggc tcttattaac ttcatgacca accgctttct
     5701 cttttacgcg attgataaac tcacgcacta ccggcagagc gacgtccgcc tccagcagcg
     5761 ccatgcgcac ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt ccacggccac
     5821 tgatattgcg cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt gtctctcgcc
     5881 tggggtggaa acggttggcc gcaatcgcga cacatcatca gtattttgcc gcagtataac
     5941 atgaaggcgt ctttgttgtt atgcaacggt tggagcagcg ttcacctgac gctatactgc
     6001 ttctctttct tattgctcaa actgtcgaca tcactatgcc cgtttttgct ctgctcgcgc
     6061 ttgtcgccta ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa aaaaacggcg
     6121 gctggcggcg catggctatt atttctgcgg tcattgcgct ggtctgccac gcaatcgctc
     6181 tggaagcccg catcctgccc gacggtgata gcggacaaaa cctcagcctg ctgaacgttg
     6241 gttcattggt cagtttgatg atctgtacgg taatgaccat tgtggcttct cgcaatcgtg
     6301 gctggttgct gctacccatt gtctatgcct ttgcgcttat caacctggcg ctggcaacct
     6361 tcatgcccaa tgaatacatc acccatctgg aagctacgcc tgggatgctg gtgcacattg
     6421 gcttatcgct cttttcctat gccacgctaa ttatcgccgc cctgtacgcg ctgcaactgg
     6481 cgtggattga ttaccaactg aagaacaaga agctggcgtt taaccaggaa atgccgccat
     6541 tgatgagtat cgagcgtaaa atgttccaca tcacgcagat tggcgtggtg ctgctaacgc
     6601 tcacgctttg cactggcctg ttctacatgc acaacttatt tagcatggaa aatatcgaca
     6661 aggctgtgct ctctatcgtg gcgtggtttg tctatattgt gctgctatgg ggacattatc
     6721 atgaaggatg gcgtggacgt cgcgtcgtct ggtttaacgt tgcgggtgca gtcattctga
     6781 cactggccta cttcggcagc cgaattgtcc agcagttaat cagctaaacc cagaaaagga
     6841 gtttccccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
     6901 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
     6961 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
     7021 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
     7081 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
     7141 ctggtgtgct gacttttgtc gtactggttt ttgctgaggt attgccgaaa accattgccg
     7201 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccc ctgcaaattt
     7261 tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg tatgctgatg cgcatgatgg
     7321 gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgcactatcg
     7381 tgcacgaatc gcgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
     7441 atctggaaaa aatgaccgtt gatgacatca tggtgccgcc agtgaaatta tcggtattga
     7501 tatcaacgat gactggaaat ctattctgcg ccaactctcc cactcacctc acgggcgcat
     7561 cgtgctctac cgtgattcgc tggacgacgc catcagtatg ctgcgagtac gtgaagcttg
     7621 gcggttgatg tcggagaaaa aagagttcac caaagaaacc atgctgcgcg ccgcggacga
     7681 gatctatttt gtgccggaag gtacgccgct cagcacgcag ttggtgaagt ttcagcgcaa
     7741 caaaaagaaa gtcggcctgg tcgtcaacga gtatggagac attcaggggc tggtgacggt
     7801 tgaagatatt ctggaagaga ttgtcggcga tttcactacg tcgatgtcgc caacacttgc
     7861 cgaagaagtc acgccgcaaa acgacggttc ggtgattatt gatggcactg ccaacgtgcg
     7921 ggaaatcaac aaagccttta actggcatct accggaagat gatgcccgca cggttaatgg
     7981 cgtcattctt gaggcactgg aagagatccc tgtcgtaggc acccgcgtgc gtattggcga
     8041 gtacgatatc gatattctcg acgtacagga caatatgatt aagcaggtaa aagtttttcc
     8101 tgtgaaaccg ctgcgcgaga gtgtggcgga gtaacgaaaa cggcccggca ttcgcatgca
     8161 gggccgtgaa ttattacgaa aacagaaatt acgcttttac tttcgctaca gttaccatcg
     8221 ccgcacgaat cgtacgacca ttcagcgtat aacccttctg cataatgccc agtacgttac
     8281 ctggcgcaac gtcatcagat tccaccattg cgatagcctg atgcacattc gggtccagcg
     8341 gcacgttagt ttcggcgatc acttcaacac caaacttacg cacaacatcc agcatcgact
     8401 tcagcgtcag ctcaatgcct tcaaccatcg cagacatatc cgggttagct ttatcagcca
     8461 cttccagcgc acgatccagg ctatcaatca ccggcagcaa ttcgttgatg aatttctcca
     8521 gcgcgaattt gtgggctttt tcaatatcca gttcagtacg acgacgcagg ttttccattt
     8581 cggcttttac acgcaaaatg ccgtcacgtt cacgggtctg ggcttcagcc agctgagctt
     8641 cgagattcgc aattttttca tcgcgcggat ccacctgctc agcagaagct tctggctcaa
     8701 ctgcctcaat ctcttcgtgc tgatccatga taatttcttc cggggcttgc ccctcaggcg
     8761 ttttctgttc tttactactc atgaatttct ccgcgttttt ttcgcattca tctcgctaac
     8821 ttcgcttatt atggggatca gtttcagggt ttcaagggaa gcactcacat tgtcatcaat
     8881 cttcgcaaca aggacctcgg aaaaatgaat aatcatttca agtgtattgg cattgtggga
     8941 cacccacggc accctactgc actgacaaca catgaaatgc tctaccgctg gctgtgcaca
     9001 aaaggttacg aggtcatcgt tgagcaacaa atcgctcacg aactgcaact gaagaatgtg
     9061 aaaactggca cgctcgcgga gattgggcaa ctggctgatc tcgcggtagt cgttggtggc
     9121 gacggtaata tgctgggcgc gacgcgcacg ctcgcccgtt acgatattaa agttattgga
     9181 atcaaccgtg gcaacctggg tttcctgact gaccttgacc ccgataacgc ccagcaacaa
     9241 ttagccgatg tgctggaagg tcactacatc agcgagaaac gttttttgct ggaagcgcaa
     9301 gtctgtcagc aagattgcca aaagcgcatc agcaccgcga taaatgaagt ggtacttcat
     9361 ccaggcaaag tggcgcatat gatagagttc gaagtgtata tcgacgagat ctttgcgttt
     9421 tctcagcgat ctgatgggct aattatttcg acgccaacag gctccaccgc ctattccctc
     9481 tctgcgggcg gtcctattct gaccccctct ctggatgcga ttaccctggt gcccatgttc
     9541 ccgcatacgt tgtcagcacg accactggtc ataaacagca gcagcacgat ccgtctgcgt
     9601 ttttcgcatc gccgtaacga cctggaaatc agttgcgaca gccagatagc actaccgatt
     9661 caggaaggtg aagatgtcct gattcgtcgc tgtgattacc atctgaatct gattcatccg
     9721 aaagattaca gttatttcaa cacattaagc accaagctcg gctggtcaaa aaaattattc
     9781 taattttacg ccagcctctt tactgtatat aaaaccagtt tatactgtac acaataacag
     9841 taatggtttt tcatacagga aaacgactat gttggcacaa ctgaccatca gcaactttgc
     9901 tatcgttcgt gagcttgaga ttgattttca tagcggcatg accgtaataa ctggcgaaac
     9961 cggtgcgggt aaatctattg caatagatgc cctcggtctt tgtctcggtg gtcgcgctga
    10021 agccgacatg gtgcgtaccg gcgctgctcg cgctgtcctg tgcgcccgtt tttctctgaa
    10081 agatacgcca gcggccctgc gctggctgga agaaaaccag cttgaagacg ggcatgaatg
    10141 tttgcttcgt cgcgtgatca gcagcgatgg tcgctcccgt ggtttcatca acggtacagc
    10201 tgttcctctg tcacaactgc gcgaactggg tcagttgctg attcagatcc atggtcagca
    10261 cgctcatcag ttactcacca aacccgagca ccaaaaattc ctgcttgatg gctatgccaa
    10321 tgaaacctct ctactccagg aaatgaccgc acgttatcag ttgtggcatc aaagctgccg
    10381 tgacctcgcg catcatcaac agttaagtca ggaacgcgct gcccgtgcgg aactgctgca
    10441 ataccaatta aaagaactta acgaatttaa tccgcagccc ggagagtttg agcaaatcga
    10501 cgaagagtac aaacgtctgg cgaacagcgg tcaattgctg accaccagcc agaatgcatt
    10561 ggcattaatg gccgacggtg aagacgcaaa cctgcaaagt cagctttaca cggctaaaca
    10621 actggtgagc gaattgattg gcatggacag caaactgtcc ggcgtacttg atatgctgga
    10681 agaagctacc atccagattg ttgaagccag cgacgaactg cgccactact gcgatcgtct
    10741 ggatctcgat cccaaccgac tgtttgaact tgaacagcgc atctcaaaac agatttcgct
    10801 ggcacgtaaa catcacgtca gccctgagac attgccacag tattaccagt cgctactgga
    10861 agaacagcag caactggacg atcaggccga ctcacaagaa acgctcgcac tggcggtaac
    10921 gaaacatcat cagcaggcac tggaaacggc gcgcgcatta caccagcaac gccagcatta
    10981 tgcagaagaa cttgcacagc tgatcaccga cagtatgcat gcgctctcaa tgccgcatgg
    11041 gcagtttacg attgatgtca aatttgacga gcatcacctg ggcgctgacg gcgccgatcg
    11101 tattgagttt cgggtaacca ccaacccagg tcagccaatg cagcctattg ccaaagtcgc
    11161 atccggtggt gaattgtccc gcatcgcatt ggcaattcag gtcatcacgg cgcgtaaaat
    11221 ggaaaccccg gcactgattt ttgatgaagt ggatgtaggg attagcggtc caacagcggc
    11281 agttgtcggc aaactgctgc gtcaactcgg cgaatcaact caggtgatgt gtgttaccca
    11341 cctgccacaa gtcgcgggat gtggtcatca acactatttt gtcagcaaag aaaccgatgg
    11401 tgcgatgaca gaaacgcata tgcaatccct gaataaaaaa acgcggttac aagagctggc
    11461 gcgcctgctt ggtggcagtg aagtcacacg taacacactg gcgaatgcga aagaactgct
    11521 tgcggcgtaa acttttttcc tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc
    11581 ggaaagcaaa aggttttaaa gtgatgaaag gtctattatc atcggcatat tacagatgag
    11641 ccacgtactg ctcgggcccg aaaaggaatc aaatcactat gcgctgtaaa acgctgactg
    11701 ctgcagcagc agtactattg atgttgaccg caggctgttc cactctggag cgagtggttt
    11761 accgtcctga catcaaccag gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg
    11821 ttggcatgac gcaacaacaa gttgcgtacg cattgggtac accgctgatg tccgatccat
    11881 ttggtacgaa tacctggttc tatgtcttcc gccagcaacc aggtcatgaa ggtgtaacgc
    11941 agcaaacgct gacgctaacc tttaacagta gcggtgtgtt gaccaatatt gataacaaac
    12001 ctgcgctgag tggtaactaa taataaagtt gctctcaaag acgttaaaaa aggtgctcaa
    12061 tgagcacctt ttttctgtcc gttatttatt cgctgatttt tctgctcgtt gcctgcgaag
    12121 ctctttcgga tcggcaatga gagggcgata aatctccacc cgatcgccat catgcacact
    12181 atcgcttaat tttgccggac ggctgtaaat gccgactttg ttttcagtta aatcgatatc
    12241 ggtacgcaat tccagcaagc cactggcgcg aatagcttct tcaaccgtcg cgccctcctg
    12301 cagcgtcacg cgctgcaggt actgcttctt aggtagcgca taagccacct caacggcaat
    12361 tttacctggc actgtaaacc tctttcgcac gaaccgtaaa agcctggacc atattagacg
    12421 ccagctcttt aaacacgcga ccaaaagcga gttcaatcaa cttattggta aactcaaagt
    12481 cgagatgaaa ctcgatacga cacgcctcct ggctcagcgg cgtaaacttc catccaccaa
    12541 tcaatttctt gaacggccca tccaccagat tcataagaat actttggtta ctggtcaact
    12601 ggttgcgggt agtaaacgtt ttgctgatcc cagccttaga gacatctacc gccgcagtca
    12661 tctgcccagg agtggactcc agaatccgac ttccggtaca acccggcaaa aactgaggat
    12721 aagactgaac gtcattcact aactgataca tttgctccgc gctgtagggt accagtgcgg
    12781 tccggctaat ctgaggcata acaatttcca tcaacaaaaa tccaacaaat aatatcattt
    12841 atcctgcagt aaaaaaaacg ctatcccggc gctgggtaac atccggttca tgctaagata
    12901 gagccttgtc ccccgcagga ttgatatggg gtattttcga tttcagatta ccgatgattc
    12961 acgacgctta tgacgaagaa aaaagcacat aaacctggtt cagcgaccat cgcgcttaac
    13021 aagcgcgccc gtcacgaata ctttattgaa gaagagttcg aagcgggact tgccctgcaa
    13081 ggctgggaag ttaaatccct gcgcgcagga aaagccaata ttagcgacag ctatgtcctt
    13141 ctgcgcgacg gagaagcgtt cctgtttggc gcaaacatca cgccaatggc tgtggcttct
    13201 acgcatgtgg tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca gcgcgaactt
    13261 gattcgttgt acggtcgcgt caatcgagaa ggctataccg tagtggcgct ctccctgtac
    13321 tggaaaaatg cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaagaa acagcacgat
    13381 aaacgttcag atatcaaaga acgcgaatgg caggtggata aagcgcgtat catgaaaaac
    13441 gcccaccgtt aaacctgcac tccaattctt gacctgttcc tcaccgcgcc tccctctccg
    13501 gcggcgcgaa tgaacatctt attggctatc acatccgaca aaaatgttgc catcccattg
    13561 cttaatcgaa taaaaatcag gctacatggg tgctaaatct ttaacgataa cgccattgag
    13621 gctggtcata gcgctcataa atctggtata cttgccgtta cacattgggg ctgattctgg
    13681 attcgacggg atttgcgaaa cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag
    13741 ccgcaaaaaa tagtcgcaaa cgacgaaaac tacgctttag cagcttaata acctgcttag
    13801 agccctctct ccctagcctc cgctcttagg acggggatca agagaggtca aacccaaaag
    13861 agatcgcgtg gaagccctgc ctggggttga agcgttaaaa cttaatcagg ctagtttgtt
    13921 agtggcgtgt ccgtccgcag ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc
    13981 gaggacgtag gaatttcgga cgcgggttca actcccgcca gctccaccac tttaagaagg
    14041 actacaaccg gacagtagca ataaatacag ccacttacgg acacaagcca gacagtagtc
    14101 ggactgaaaa aagacaaaaa tatacacgtg aaatgcacgt gcacttctaa aaaaccccag
    14161 ttatcaccga ctggggtttt tcaatttgta actaagggta acaacccccc cgtttttttc
    14221 atctcactca ccttgatact gtttatttgt acagttaaaa ataatactgt atacaaacac
    14281 agtatagagg gacttttatg cgtattgaga tctgcatagc caaagaaaaa atgactaaaa
    14341 tgccaaacgg tgctgtggat gcgttaaagg aagaattaac ccgacgcatc agtaaacgtt
    14401 atgacgatgt agaggtgatc gtaaaagcca ccagcaacga tggcctttct gttacgcgca
    14461 ccgccgataa agattcagct aaaacttttg ttcaggagac tctgaaagat acctggaagt
    14521 ctgctgacga gtggtttgtt cactaattaa cacgtaaaat cggtaacggc tggaaatcat
    14581 tcaatactcg cactatcgaa agttcaccag ccaaccgcag cacgtcctgc atacgtcgtg
    14641 tctgcggttt ttctttttcg cttacattgt gtctggttct tccggccact caatatcagg
    14701 tgcagttgat gtatcaacac ggttcagcaa cacccgatac tttttctagg cttccagcaa
    14761 tgaggtttct tcctccgttg caatttccag atctgcagca tcctgaagtg gggcgcacca
    14821 cgcaaacatc acaaaatatt gtcgctgcga tgaacctgta caataaataa tgaatcacgg
    14881 tacgtttctg tgtataacgt actgtagttt tctttacgat ttatagctgt actggtgagc
    14941 catgagtaat ctgaatccat gcatggcgtg tggtgccgtt gtgcattttc tgcgtctctt
    15001 ttactggtcc gaagccgatg atgctgaagc agtcctctcc tctagcctca tccctgtata
    15061 gcacgcatca aataatctgg gatatcattt cgtagtttta caatatcaat atccgaatcg
    15121 atatgtttct ggtaaaactt gtccgcaagc aagtttgcta tggtatctga tgtacgattt
    15181 tttacatccc ggctcatacc atcacaaaga gcatccattt tcttgccaaa agaagcactc
    15241 cccattatta catgattaat agaactttgt gctgccttaa catcaagggt tagttgattc
    15301 tcctggctca caagaaaaaa gactctttgc ctctcttcag tggaaatttt tgcatttttt
    15361 tgaaaacagt aattatcaat ctcctggttg atcacccttg agggatatac tatccgttca
    15421 tcttttatcc actcatcaat atctttagta ctctgccttc tcaccgtttc cagtatattg
    15481 ccgctaaatt tgcgaacaga aaagctaata gtccgttctc caattgaaca ttttacgacc
    15541 tcatcattct tccctcggaa agagttcctg aaggagttct ctctatggca acttatacca
    15601 aaagagctga aa
//
LOCUS       DBFIFS010000100        15565 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.100, whole genome shotgun sequence.
ACCESSION   DBFIFS010000100 DBFIFS010000000
VERSION     DBFIFS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.100"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(105..1508)
                     /gene="yihO"
                     /locus_tag="N7A07_002758"
     CDS             complement(105..1508)
                     /gene="yihO"
                     /locus_tag="N7A07_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477391.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            complement(1548..2933)
                     /gene="yihP"
                     /locus_tag="N7A07_002759"
     CDS             complement(1548..2933)
                     /gene="yihP"
                     /locus_tag="N7A07_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477392.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            complement(2979..5015)
                     /gene="yihQ"
                     /locus_tag="N7A07_002760"
     CDS             complement(2979..5015)
                     /gene="yihQ"
                     /locus_tag="N7A07_002760"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU2477393.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            complement(5123..5998)
                     /locus_tag="N7A07_002761"
     CDS             complement(5123..5998)
                     /locus_tag="N7A07_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU2477394.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            complement(6059..6961)
                     /gene="yihR"
                     /locus_tag="N7A07_002762"
     CDS             complement(6059..6961)
                     /gene="yihR"
                     /locus_tag="N7A07_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU2477395.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            complement(7028..8269)
                     /gene="yihS"
                     /locus_tag="N7A07_002763"
     CDS             complement(7028..8269)
                     /gene="yihS"
                     /locus_tag="N7A07_002763"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGU2477396.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(8285..9163)
                     /gene="yihT"
                     /locus_tag="N7A07_002764"
     CDS             complement(8285..9163)
                     /gene="yihT"
                     /locus_tag="N7A07_002764"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU2477397.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(9187..10083)
                     /gene="yihU"
                     /locus_tag="N7A07_002765"
     CDS             complement(9187..10083)
                     /gene="yihU"
                     /locus_tag="N7A07_002765"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU2477398.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            10251..11147
                     /gene="yihV"
                     /locus_tag="N7A07_002766"
     CDS             10251..11147
                     /gene="yihV"
                     /locus_tag="N7A07_002766"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU2477399.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            11181..11966
                     /gene="csqR"
                     /locus_tag="N7A07_002767"
     CDS             11181..11966
                     /gene="csqR"
                     /locus_tag="N7A07_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2477400.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            12065..12664
                     /gene="yihX"
                     /locus_tag="N7A07_002768"
     CDS             12065..12664
                     /gene="yihX"
                     /locus_tag="N7A07_002768"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU2477401.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            12658..13530
                     /gene="yihY"
                     /locus_tag="N7A07_002769"
     CDS             12658..13530
                     /gene="yihY"
                     /locus_tag="N7A07_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU2477402.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            13527..13964
                     /gene="dtd"
                     /locus_tag="N7A07_002770"
     CDS             13527..13964
                     /gene="dtd"
                     /locus_tag="N7A07_002770"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU2477403.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            14009..14950
                     /gene="fabY"
                     /locus_tag="N7A07_002771"
                     /gene_synonym="yiiD"
     CDS             14009..14950
                     /gene="fabY"
                     /locus_tag="N7A07_002771"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU2477404.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            15361..>15565
                     /locus_tag="N7A07_002772"
     CDS             15361..>15565
                     /locus_tag="N7A07_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477405.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLF"
BASE COUNT         3900 a         3863 c         4011 g         3791 t
ORIGIN      
        1 agagaagata ataaaattat tgcattaata tttttcataa taagtccatt tttaaacgta
       61 gggaggccgc cacccaccag gatggaaata gcggctattg acatttataa cgttacggaa
      121 gccgtttttt cttctgtcgc catctcttta tttttgcgtt ggttgatttc ctcaataatc
      181 aacgcaaacc gtttttcatt gagtgtgtaa aagaatccca tcgtcaatgc agcaataatt
      241 gccagtgcac aaggccagat aaaaatgagc tgacgcaaac cctgtaatgt cgcatcactt
      301 tgcgcgatgt tgggaacata accgatttgc gtcagcataa tgcctggcaa gaagccagca
      361 agtgcggcag aaattttacg aaagaaggta taaccggtat aaacagaacc ttcggcacga
      421 ataccggttt tccattcacc gtaatctacg gtatctggca ccagtgccca gttcaggctg
      481 tttacaaagg ccgtgccaaa aaaggcgata caagagaaca tgacgaaagt gaaagagtta
      541 cttccccaga aataattcag gatatcacca accgcccata gcaccatgcc agctaaataa
      601 acctgttttt taccaaaaca ttttacagtt aatggcacca gtaatacgcc gacgaggatg
      661 catcccatac tgaagaatcc catccacgac aataaattaa tatcattcag cacatactgg
      721 gtgtaataga cctgaatcgc cagtttgata ttaaatgccg ccagggtaca caagttagca
      781 atgcataaaa ccagcaatgg cggattccga aatatcgcgc aaaaagattt gagaatactg
      841 gctttatgtc cggttggtgc tgtgtccaca taatgctctt tgacgcctct gtagcacagc
      901 atcataaaaa taaagccacc aatggagaac atcagtgccg cacaggcata accgacggtt
      961 gagtcagaaa aaagcgactg caacggaata aacgccacgg tacaaatcaa tagccctatg
     1021 gttgcaccac cctggcggta agccgcgagc tgcgcgcgct cattcgggtt tttagtaatg
     1081 gccgggatca tcgcaccata cgagcagttc atcagactgt atgaaagtcc aaacatcata
     1141 aacagcgcgg tggcaatcgt cgttttaacc ggcaaactaa aagtggtggc gataaactgg
     1201 agcgttgcaa ttaacgccgc cggaaccgct gcgtataaaa taaagggacg aaatttgcct
     1261 ttaggcccaa tatttttccg cgaatcgagt aaaaatccgg tgagcatatc ggtaaacgcg
     1321 gtaaaaaact tcgccaccag aaagataatc ccgccatagt aagctggcat ccctaattca
     1381 tcggtataaa atttgaggag atacagagta ccgatgctaa gcattaaatt cgaaccaacg
     1441 tcgcccatac cataggcgat tttttcccgc agattcagtt ttaatgtcag tggattatgg
     1501 tcagacataa tcactcctta taacgccccg cagggcattg tttattatta cgccgtgcgt
     1561 ttacgggctt ctatttcttc aacaatgcgg acatacatct tctcgttcag gctgtagaag
     1621 cagcccatcg ccacaatggt gactaccgcc agtgcgcttg ggtagatgaa gatcagctgg
     1681 cgcaaccctt caatggtgtg gtcagcctgc gccacgttcg gcacataacc gatttgcgtc
     1741 agcatccagc cggggaagaa accagccagc gcctgagaca ctttgcgaaa gaaggtgaag
     1801 ccggtgtaga ccgttccttc cgaacgaacg ccggtacgcc actcgccgta ctcgacggtg
     1861 tcggaaacca gcgcccagtt caggctgtta acaaacgctg agccgaagaa tgccaggcag
     1921 gagaacgcca cgaagctgac cgaaccgccg ctgaagaaat agttgagcag atcgcccagc
     1981 acccaaatca gtaggccgcc gatatagacc ttcttcttac caaaacgcct gactgcgcca
     2041 ggcatcagga acacaccgat gaaaatacag cccatgctga aaaatcccat atatgacaac
     2101 aggatgggat cgttaagtac gtactgggtg taatagacct ggatggcgag cttgacgtta
     2161 aacgccccta aagtgcagag gttggcaatg cacagaatga acagtgggcg gttaccggca
     2221 attgcgcgga aagattgcag caggccaggc ttttgcgccg gattggctgg ttgggtttcg
     2281 acataacgct ctttcacgcc cgagtagcag atccacataa acagcaggcc gaacagtgaa
     2341 aacagcgtgg cggcgaagat atagccaagt tgctgattac cttcgataag attcataacc
     2401 ggcacgaatc ccaccgtgca cagcagcagg cccagcgtag cgcctccctg acgccatgcc
     2461 gccagtgatg cgcgctcgtt ggggtttttg gtaatagcag gcaccatcgc gccataggag
     2521 cagttcatca tgctgaaaaa cagcccgtag agcataaaca gaatagtggc catcaccgtt
     2581 ttaccagtga catcgaacgg tgtgccgata aagttagcga ttgccaataa ggtgaccggg
     2641 aatgacgcgt acaaaatgaa agggcggaac ttacctttcg ggccaatctt acgccgggaa
     2701 tccaacatga tgccggttcc catatcggta aaggcggtaa agaatttcga gatcaaaaag
     2761 ataatgccgc cataggtgcc aggtagcccc agaacgtcgg tataaaactt caaaagataa
     2821 agcgtaccaa tatccagcag gatgttagag gccagatcgc cgataccgta agagagtttc
     2881 tctttaaagg gcaggcgcaa agttgccgga tcttccgttg tgatgtgact catcgttatt
     2941 ctccatttgc ccgtgagttt ccccacgggc gggcatgatt agatgttttt taacgaggcg
     3001 aacagtgcag cccattcgct gtcggcgcga taaaagaccg gcggcttgcc gatgggcgct
     3061 tcaacagtta tttctccacc gtggaaggtt tctccggtcc aggcgtggac ccagttatcc
     3121 tctggcagat agagcgtcca gtcgctgcga ccttcttcgt gtaccggagc aaccagaatg
     3181 tcgcgaccta acaggtactg atatttcagg gaataggtct gcgcgtcgtc ttcgtaatgc
     3241 aggaacagcg ggcgcataac cggcaggccg gacttcgcat tcagcgcgac ggcctctttc
     3301 aggtaaggtt tcagggtggt gaagacggtg gtcatacggg cgaaatgggc gatggtttcc
     3361 gcgtcgccgt caaactgcca gttgtcgcca ggacggttac cttcgtgggt gcgcatcatc
     3421 ggcgtgaagg cgctgaaatc gcaccagcgc agcagcagct ctttgctgcg cttcatctca
     3481 aacagggtgg tataaccgcc aatgtcgctg tgatgcaggc catgtccggt cattgccaga
     3541 gatagcgccg ccgggacaac cgacgccagg ccatcgtcca gactccagtc cacgttctgg
     3601 tcacccgccc acatcatggt ggagtatttc tggctaccgg tagaaccggc gcgcataaag
     3661 aagaggatct cgccgagctt gcccgtttct tcaagggctt cgtagttaca tttcgcccac
     3721 agcgcgggcc aggcgttatg cataatttcc gcgctgatgc cgttatgcag gtacgtgtcg
     3781 gtgggcagat actcgccgaa gtcagccatc cagccgccgc agccgagttc aatcatgttc
     3841 tttttgataa cttccttgaa ccaggcgtag gcttccggat tggtgagatc gacaacgccg
     3901 ccgtagaact cgccaaactc caccagatag tcgccaccgg cgacatcttt tgccagataa
     3961 ccgcgctttg ccgcctcttc gcagagatct ttatcactgg caacatacgg gttgatatag
     4021 gcaaggaact gcacgccctc tttgttccac tgcttgatac gcgaatccag ttgcgggtag
     4081 ttttcgctgt tccacttcca gttccacatc acgcgtttgc caaaagaggt catacgaata
     4141 ccggaccagt cctgcgccca gatgccgttg accttcacgc ccgcgttacg catggtgtcc
     4201 agtttcttct ggcacacttc cgtcccgccc tgaatgccga gcgttactcc gtcataaatc
     4261 cagtcgggca gttctggctg gcgtcccagc agggcggtta atttttccag cagggagatg
     4321 tatgtgtcag cacattcaaa acgcagcgtt gctttgtctt cccacagcgc cagttcatgg
     4381 tattccgggg cactaaagtc gaagttcata tagcaactgt tatcaacatg gcaataatac
     4441 ttctgcgtgc tgacaaacgt aggctgtggg aagaaagtcc agtaatagtc gccgcccgca
     4501 ttttctttgc agtcggcctg ccaggtgaca taggtttgtt tgttgcgacc aacgccttgt
     4561 tcactggtcc atagcgggaa cggtttgccg cgcagatcga agtaggaaaa ctgttcgccg
     4621 cagccgtaga tatggtcctc tggttgagcg gcaaggcgca gccagatgcg gttgtggtta
     4681 aggttgtcgt tttgtagttc cagcaataaa cggccctgat cgtcggcaga gatattcagc
     4741 gtggcgctaa tgtcagaacc acgactgaaa tggattaacc aaccatccgg tgactggctg
     4801 acgatggcgt cggtaagcgc aattttctcc tgtagtttat ctttaatgct gaaattgccg
     4861 cggaacatat cgatgtccgc tatacctgag ccaatccata aacaaggatt atctttgcta
     4921 tgggttaaaa taagacgttg tcgaaaatgt agggtaaaac tgtcgttatt ctgatgaaat
     4981 tcaaaatctg gtaattgtgg gcgtggcgta tccataaata atctccatag agtataaagc
     5041 taatatttca tgccatcctg ttattacagg tttaattaat taaagaatgt gtgcggatat
     5101 aaatttaaac ccgcataagt gattatttac ttgcttcagc ctgacggatt gccgccactt
     5161 ttttaccaaa taccagttgc agtataacta acaaggtaaa cggaggtaga agtgcggcag
     5221 cggcgggaat tcccagcgcg ccttttatca acatggaacc ggaggcaatc agcaataaaa
     5281 agaccatcac acggaacact ttgactgaaa tacgtttatg cacatattgt ccaaccagtg
     5341 aaccgaggat catggtggga atacaaaaca gcaccagttt aaagatcgaa acagtcaaaa
     5401 ttccgctggt cgccagcccg ccgatcaccg cgatgttgtt tgcggtgaag aaagcgttca
     5461 gagtgccacg aaatgccgcc gggcctaagt tacgcagcat accgtagata accatcggtg
     5521 gtccgttggt tgagaaagct gcgcctaatg cgcctgctac tgcaccgata ggtgcggcga
     5581 tccagctacg gtcataaatc ggcaggcgtg gaataaacat actgtaaaag gagtacagga
     5641 taaggaatcc gcctaagccc actttcataa aatggtcggg taaatacgac agtgcatata
     5701 aaccgacggg tattcccaga aacgagaaaa taatcagtac cattgccgat cgccaatccg
     5761 tttctttacg tgacagccag gtggcgtaca aggcggtcgc ggtgccgaca attaccgaga
     5821 gtggtgttgc catttttacg gggagcatta acgtgacgag cggcatagtg gttaaaccac
     5881 cgccaaatcc cgcgcatatc cccacaaagg tataaagaaa cattaacgcg ataacgatac
     5941 ataccacgtt tgtttccagc atgaccccat ccggtgaaaa taacgtaaaa aagtccataa
     6001 tatttcctgt tgtgtaaata caactctaaa caatgatctc gttcttttct taataaaatt
     6061 aattgtgttg cccgtaaata ttaaaaaaca ggcggtgcgt tttccctggt tccagtaata
     6121 ataagtcgag acctgaatta aaggcgttag gcgggcaact cataggttca actgccagtc
     6181 cctgacgctg taatttttca ccgctataaa tttgtaccca gggttgatcg gaacaaaggc
     6241 taaccgaaag cgcctgttgt ggatgggtaa tggttatttc ccacagatca ttagcggttt
     6301 taaaggtgtg gtcgattttt gtcgcggcta tttttttcgc ctgcgtgaaa tttaaatcca
     6361 gctcatcaac gtggtgaagc gttgtcggat tagcgtgctc atcgacggca taaacctggt
     6421 ttgccggaag ctggaacaga tattcatcaa cagatgttaa gttgcaggtt aaataaggat
     6481 gaatacccac accatagggt gcggtcacag taccaatatt ctgacttgcg atctcaacgg
     6541 aaagacccgt gcgtgcattg agcgaataaa caacctgcga agccagcata aacggatagc
     6601 cgtaactggg cggcagaaaa gctgtcaggg taacgctggt ggcggttaat tcgctgattt
     6661 gccagtcacg ccaggcgagc agcccatgaa tagcggcttt tgaaccatgt tcattaatgg
     6721 gtaactgata ttcctgcccc tgatagcgat aacagccgtt ggcgatccgg ttcggccacg
     6781 gaataaggac tttgcccaga tgtgccagcg gcatctcttc gggtttatgt ggaataacta
     6841 aatgacagcc ctgaaaggtt aattcagcca gacctgcacc gacggtcaca atggtcgcgt
     6901 gataatcccc cgcggcaagt gaaacggtct gtccactgca atgcatgttg gtgatttgca
     6961 tacgaggtac ctttattgaa taacgacata cccggcgtgc agcctgatat gtcgtttttt
     7021 tagtcagtta tttcgcgtta atatccagca gacccgccgc aactgccgga gccaggcctg
     7081 gcgctaacgg gatacgcggg atcaccaggc aatgcagcag gtgataaata tcctgtttgc
     7141 cgtcccagac tttggtggtg accttattgt ccgcatccag ctcctgccac catgaaccat
     7201 tttcgtagtc catcaggtat ttgatgcagt agtcccacca tgtctgatac caggtttcgt
     7261 actggcgatc gccggtgacg gtgtagagcg cgtaggccgt acccatcgct tcgacgatag
     7321 gccaacgcac acgttcgcgg accaccggtt ttccttccca gtcaacggta taaacaatcc
     7381 cgtccgcacc atcgggtgcc caggcatcac gcacggtggc gttaaacaga cctttggcat
     7441 cttctagcag ccatgctggc ggttgttcgc aacgggcttc cagggccgcg tggatgtgca
     7501 gcattaaacg gccccattcg atccagtggc ctggtgtacc tccgaacgcg cggaagcggt
     7561 gcgccgggtt atctttgttg taatccggca gcggattcca ctgggtatcg aagtgttcgt
     7621 taacgcgata atgattattt cttgcgacgt cgtggataat cacggaagcc acgcgaatcg
     7681 cgcgatccag ccattttttg tcatgcgtta cgtcataaac aattaagaaa gcttccactg
     7741 cgtgcatatt ggcattgccg ccgcggtact cttcggtttt gctgaaggct tcgtcccagg
     7801 actccaggca catctgctct tcttcgctcc agaagtattt ctcgataatt tcaatggtgt
     7861 aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac ggcgctggca gcacccagca
     7921 gagcaaagaa atgttgatag ccctgtttgg aggcatccac cacgccctcg tcattcacac
     7981 aggcatacca gccgccatat tttttatcgc gcagtgcgcc gttcatggct ttaataccgt
     8041 gatcaaccaa cgagtaagcg ccaggtcgac ccatcgccgc agcaacggaa taaacgtgca
     8101 acatacgagc tgtgatccac agatgggtgc ccatctcttc tttgatttgc cctttattgc
     8161 ctaaccagcc aaatccggtc ggtactacgg aatttttacc aaaatcaaag atgcgatcgg
     8221 tttcctgttc cagccaacgg ttgtgactta aagtgttaaa ccatttcatt tttgtatccc
     8281 cattttaacg gcgtttagcc atcatttcgt cgacgatatc gccaagttgt tgtaatttcg
     8341 gtgcgcaaac gtcacgcagc atcagctcgt tgtctggtaa accgacgacc gatgcccaga
     8401 cagcacgacc agccaggaag cccgatgcac ccgctgtcat tgccacgcgt acggcacgcg
     8461 ggaacagttt ttcgtcgaca ccggaagaga ggatcaccca cggcatattg atatggtcat
     8521 tcagacgttg tgaagcactg agaagctctt gttgcggacc tttaccgtaa agcggcattt
     8581 caactttgta gaggtcagcg ccactgtcgc ccagctcttt ggcggcatcg atgatcgctt
     8641 gttcgcgatc gaatttatcg ccacgacgtg gtgggcggac gactggctca ataatgctta
     8701 ccaggccgtg tgagtggcac agttcgttaa actctttcac catatccagg cgttgctgcg
     8761 catcttcatc actacgccac agcaccagca gttttaaggc tttgccaccg tcttgtttga
     8821 tctgtagcgg attgattttg cggtcaatga ccacgctatc taccggaata ccgttgccag
     8881 gaatgaactc atcggcggcg acaatcatgg cgcaactttt ggcaatcgcg ttttgctcaa
     8941 ccacctggcg gtaacagaat tgttgatcta ccagaatcgc cgaggcataa ggcgagaggg
     9001 tcttcgccgc gttaactttg aaatcagtta aaacgctatc ggctaccgga gcgggtgccc
     9061 cagccgcggc aaacatcatg cgcatggctt cgcgctgatc gaccgccagc atggcaaaac
     9121 cgcccgatgc gcgcgtaatg tcgttgatgg tgtacttatt cattcattta ttcctttatc
     9181 cagtcgttac atttttactt tggcagtcat cccggcactg acacggacct gttccagaat
     9241 ggcggaccag tcctggcgac cgcgacccgc tgcgcgcgcc tggctataaa cctcccgtga
     9301 ggcggccccc agcggcattg gcacatgcag ctggttggcg acatcaaggg cgatgccaag
     9361 atccttatgg gcaagatcga tcatgaaggc gggagaaaga tccccgctga ggactttgtt
     9421 cggccaggaa gtggtgaagt ggcctttacc ggcggcggta ccgctcatca ctttgacggc
     9481 aacatcgaag ggaagattca gggcttcgca caaaacggca gcttctgccg aaagcgcatt
     9541 gagcgcgatg ctcatatagt tgttgatgag cttaacgcgg atccccatgc ccggaccgcc
     9601 tgcgttgatc aactcactgc ccatcgccat caggatcggc gtggcacgtt caacttgttc
     9661 agcggtgccg ccagccagca gtaacagagt accggtaatg gcatttgcag aagtacggcc
     9721 taccggaaca tccatcatgt tgaagccttt ggcttgcata tcggcaatca atttatcggt
     9781 ttgcagcgga tggatggtcg acatatcaat gaccagcgca tcggtagata agctttcgca
     9841 aacgccgttt tcaccgaaca acacgctgcg caccagatcg ccattcggca acatggtaat
     9901 gataaattcg gcatctttag cggcctgcgc cgggttggcg gcgggagtcg cgcctttgtc
     9961 taccagatgc cgcacagcct cggcattcac atcaaagacg cgaagttggt gcccttgctg
    10021 caataaattg ctcgccattg gcgaacccat ttgtcctaaa ccgataaacg cgattgctgc
    10081 cataaccttc tcctgaatac agtgatgtca ctttttgtca tttatgacac gctttgattg
    10141 tctgtttttg atcgtatttg taatttatag tcaaaaaatt gacagacgtc actttttaaa
    10201 caattggtga aattaaaatg aacgcatccc aaaatgttta aggaatgacc atgattcgtg
    10261 ttgcttgtgt aggtataacc gtgatggatc gcatctatta cgtggaaggg ttaccgacgg
    10321 agagcggtaa atacgtggcg agaaattata cggaagttgg tggcgggcca gcggcgacgg
    10381 cagcggttgc ggcggcaaga ctgggagcgc aggttgattt tattgggcgc gtcggtgatg
    10441 acgacaccgg caacagcctg ctggcagagc tggaatcctg gggcgttaac acccgctaca
    10501 ccaaacggta taaacaggcg aaatcgtcgc aatccgccat catggtggat gccaaaggcg
    10561 agcggataat cattaactac cccagcccgg acctgctgcc tgacgcagag tggttggagg
    10621 aaattgattt ctctcagtgg gatgttgtgc tggcagatgt acgctggcac gacggcgcta
    10681 aaaaagcctt cactctggcc cggcaggcgg gcgtgatgac cgttctggac ggtgatatta
    10741 cgccgcagga tatcagcgag ctggtggcat taagcgatca tgcggccttt tcagaaccgg
    10801 gtctggcgcg cttaacgggc gtgaaagaga tggccagtgc gctaaaacag gcacaaacgc
    10861 tcacaaatgg tcatgtctat gtgacccagg gcagcgcagg ctgtgactgg ctggaaaatg
    10921 gcgggcgtca acatcaaccg gccttcaaag ttgatgtggt agataccaca ggtgcgggtg
    10981 atgtttttca cggcgcattg gcggtggtgc tggcaacagg tggggattta gcggagtcag
    11041 tccgcttcgc cagcggtgta gcggcgtcga aatgcacgcg tcccggtgga cgagccggga
    11101 tccctgactg tgatcaaacc cgatcttttt tgtcactttt tgtataaaat gccagggtga
    11161 tggtttttcg aggaattttc atgagcctta ccgaactaac cggtaacccg cggcacgacc
    11221 aactcctcat gctgatcgcc gagcgtgggt atatgaatat tgatgagctg gcaaatctgc
    11281 tggatgtctc cacgcagacg gtgcgccggg atattcgtaa attaagcgag caaggcctga
    11341 ttacgcgcca tcacggtggc gcgggtcggg cttccagcgt cgttaatacg gcgttcgagc
    11401 agcgtgaggt ttcgcaaacc gaggaaaaaa aagcgattgc cgaagcggtg gcagactata
    11461 ttcctgatgg ttcaacaata tttatcacca ttggtacgac cgttgagcat gttgcccggg
    11521 cgttacttaa ccataatcat ttgcggataa tcaccaacag cctgcgtgtg gcgcatattc
    11581 tttaccacaa cccgcgcttt gaagtgatgg tacccggcgg tacgttgcgc tctcataata
    11641 gcgggatcat tggcccttca gcggcgtcct ttgtggccga ttttcgtgct gattatctgg
    11701 taacaagcgt tggggcgatt gagagcgatg gcgcgttgat ggagtttgat gtaaacgaag
    11761 ctaacgtggt gaaaacgatg atggcgcacg cgagaaatat cctgctggtt gccgatcaca
    11821 ctaagtatca tgcttcggcg gcggttgaaa ttggtaacgt ggcacaggtc accgcgctct
    11881 ttaccgacga gctgccgccc gctgcgctaa aatcacgctt acaagacagc caaattgaaa
    11941 tcatccttcc ccaggaagac gcgtagattt ttgtgactgt tgacctggct tcataccttg
    12001 ccacatagcc aaacccatcc tttcccgcta cagttaattt cttgtggcgc gaaaggaggc
    12061 aaaaatgctc tatatctttg atttaggtaa tgtgattgtc gatatcgact ttaaccgtgt
    12121 gctgggaacc tggagcgatt taacgcgtat tccgctggct acgcttaaga agagttttca
    12181 tatgggggag gcgtttcatc agcatgagcg tggggaaatt agcgacgaag cgttcgcaga
    12241 ggcgctgtgt catgagatgg ctctaccgct aagctacgag cagttctctc acggctggca
    12301 ggcggtgttt gttgcgctgc gcccggaagt gatcgccatc atgcataaac tgcgtgagca
    12361 ggggcatcgc gtggtggtgc tttccaatac taaccgcctg cataccacct tctggccgga
    12421 agaatacccg gaaattcgtg atgctgctga ccatatctat ctgtcgcaag atctggggat
    12481 gcgcaaacct gaagctcgaa tttaccagca tgttttgcag gcggaaggtt tttcacccag
    12541 cgatacggtc tttttcgacg ataacgccga taatatagaa ggagctaatc agctgggcat
    12601 taccagtatt ctggtgaaag ataaaaccac catcccggac tatttcgcga aggtgttatg
    12661 ctaaaaacca ttcaggacaa agccaggcac cgtacccgtc cactatgggc ctggctaaaa
    12721 ctactctggc aacgcattga tgaggacaat atgacaaccc tggcaggtaa ccttgcctat
    12781 gtgtcgttgc tctcattagt gccgctggtt gccgttgttt ttgcgctttt cgccgctttt
    12841 cccatgtttt ccgacgtcag cattcagtta cgtcacttta tttttgccaa ctttctgcct
    12901 gctactggcg atgttattca gcggtatatc gaacaatttg ttgccaattc caacaagatg
    12961 accgccgttg gggcgtgcgg gctgatcgtc accgcgttat tgttgatgta ctccatcgat
    13021 agcgcgttga ataccatctg gcgcagtaaa cgagcgcgac ccaaaattta ctcgtttgcc
    13081 gtgtactgga tgattttaac gctggggccg ctgctggcag gggccagtct ggcgatcagt
    13141 tcctatttgc tctccctgcg ctgggcgagc gatctcaata ctgtcatcga caacgtgctg
    13201 cgtatttttc cgttgctgtt gtcgtggatc tccttctggt tgctgtacag cattgttcct
    13261 accatccgcg tacctaaccg cgacgcgatt gtcggcgcgt ttgttgctgc actcctgttc
    13321 gaagcaggaa agaaaggttt cgcgctttat atcaccatgt tcccgtcata tcagctcatt
    13381 tacggtgtgc tggcggtgat ccccattctc tttgtttggg tctactggac atggtgtatc
    13441 gtcttgcttg gcgcggaaat tactgtcact ctcggggaat accgcaaact aaaacaagca
    13501 gctgaacaag aagaagacga cgaaccatga ttgcattaat tcaacgcgta acccgtgcca
    13561 gcgtcaccgt ggagggagaa gtgacgggcg aaattggtgc gggacttttg gtgttattgg
    13621 gtgtcgaaaa ggatgacgac gaacagaaag caaaccgtct gtgcgagcgt gtgctcggct
    13681 accgcatctt tagcgatgcc gaaggcaaga tgaatctcaa cgtgcaacag gcgggcggca
    13741 gtgtgctggt ggtttcccag tttaccctcg ccgcagatac cgaacggggg atgcgcccaa
    13801 gtttctccaa aggtgcatca ccggatcgcg cagaggcgtt atatgactat ttcgtcgaac
    13861 gctgccgtca gcaagagatg aacacgcaaa caggacgctt cgctgcggat atgcaggtat
    13921 cgctggtcaa tgatggcccc gtgacattct ggttgcaggt atgagccagc ttccagggtt
    13981 gtcacgggaa acaagagaga gtatcgctat gtatcacctt cgggttccac aaacagaaga
    14041 agaattagag cgttactatc agtttcgctg ggaaatgttg cgtaagcccc tgcatcaacc
    14101 aaaaggttcg gaacgcgacg cgtgggatgc gatggcgcat caccagatgg tcgtcgacga
    14161 gcagggtaat ctggtggcgg taggccgact gtatattaat gccgacaatg aagcgtccat
    14221 tcgctttatg gccgttcatc ccgacgtgca ggacaaaggg ttaggcacgc tgatggcgat
    14281 gaccctggag tcggtggcgc gtcaggaagg cgttaagcgc gtgacctgta gcgcccgtga
    14341 agacgcggtg gagtttttcg ccaagctggg gtttgttaat cagggagaaa tcaccacgcc
    14401 aaccaccacg ccgattcgcc attttttgat gattaagccc gtcgccactc tggatgatat
    14461 tttgcatcgc ggcgactggt gcgcgcagct gcaacaggcg tggtacgaac atatcccgct
    14521 tagtgaaaaa atgggcgtgc gcattcagca atataccggg caaaaattta tcactaccat
    14581 gccagaaacc ggcaatcaga atccgcacca tacgctgttt gccgggagtt tattctcact
    14641 ggcgacgctc accggttggg ggcttatctg gctgatgctg cgcgaacgcc acctcggcgg
    14701 aacgattatt ctggcggatg cgcatatccg ctacagcaaa ccgattagcg gtaaacccca
    14761 tgcggtagcc gaccttggtg ccttaagcgg cgatctcgac cgtctggcgc gcggacgaaa
    14821 agcacgggtg cagatgcagg tcgaaatctt tggcgacgag acgccgggtg cagtgtttga
    14881 aggcacgtat atcgttctgc ccgcgaagcc atttggcccg tatgaagagg gcgggaacga
    14941 agaagagtag ctgatggaga ggggcggtgc tgcctctctc attcaggtca tttatattag
    15001 tccttggcta tttcggttat attgaataag aactctataa atttttattc ttcgttggct
    15061 ccatacgtgt cttgctacat cactgttcag gagcatttaa atattatttg tagagctgta
    15121 tagcaattat cgcagataat gcttaattca tcatcttagt tcaggtttga gcgatttttg
    15181 atagtcaaaa ccccctctgg tgccatagtt ttctcataaa tactttaaaa atctatagct
    15241 aacaaacatt atgcaaagat aatgggctgg catcattgtc agtatcattc atttactgct
    15301 ctggatgtta taaataatta tgtagcatgt ttgctcttaa accgtgggaa ggaggctgta
    15361 atggttgcca atattaatct cattaaacaa gaaagttatt ctgttgttaa tttagaaaaa
    15421 caactaatga gtaatgaatc accgaagcaa tcgctattaa atgatttttc acatcgcctt
    15481 aaaatggaag gtgaggaaca actgttccat tgtgttaacg aactgaagta tggtttgtcg
    15541 agtaataatg aatatgacct gttta
//
LOCUS       DBFIFS010000101        15509 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.101, whole genome shotgun sequence.
ACCESSION   DBFIFS010000101 DBFIFS010000000
VERSION     DBFIFS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.101"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            191..757
                     /gene="yceJ"
                     /locus_tag="N7A07_002773"
     CDS             191..757
                     /gene="yceJ"
                     /locus_tag="N7A07_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU2477406.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            761..1336
                     /gene="yceI"
                     /locus_tag="N7A07_002774"
     CDS             761..1336
                     /gene="yceI"
                     /locus_tag="N7A07_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU2477407.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1378..2430)
                     /gene="trhO"
                     /locus_tag="N7A07_002775"
     CDS             complement(1378..2430)
                     /gene="trhO"
                     /locus_tag="N7A07_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU2477408.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2655..3575
                     /gene="lpxL"
                     /locus_tag="N7A07_002776"
     CDS             2655..3575
                     /gene="lpxL"
                     /locus_tag="N7A07_002776"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU2477409.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3747..4973
                     /gene="mdtG"
                     /locus_tag="N7A07_002777"
                     /pseudo
     CDS             3747..4973
                     /gene="mdtG"
                     /locus_tag="N7A07_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            5056..5430
                     /gene="msyB"
                     /locus_tag="N7A07_002778"
     CDS             5056..5430
                     /gene="msyB"
                     /locus_tag="N7A07_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU2477410.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5431..5658)
                     /gene="yceK"
                     /locus_tag="N7A07_002779"
     CDS             complement(5431..5658)
                     /gene="yceK"
                     /locus_tag="N7A07_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU2477411.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5831..8374)
                     /gene="mdoH"
                     /locus_tag="N7A07_002780"
     CDS             complement(5831..8374)
                     /gene="mdoH"
                     /locus_tag="N7A07_002780"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU2477412.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8367..9902)
                     /gene="mdoG"
                     /locus_tag="N7A07_002781"
     CDS             complement(8367..9902)
                     /gene="mdoG"
                     /locus_tag="N7A07_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU2477413.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10295..11452
                     /gene="mdoC"
                     /locus_tag="N7A07_002782"
     CDS             10295..11452
                     /gene="mdoC"
                     /locus_tag="N7A07_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU2477414.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11460..12941)
                     /gene="clsC"
                     /locus_tag="N7A07_002783"
     CDS             complement(11460..12941)
                     /gene="clsC"
                     /locus_tag="N7A07_002783"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU2477415.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12883..13416)
                     /gene="ymdB"
                     /locus_tag="N7A07_002784"
     CDS             complement(12883..13416)
                     /gene="ymdB"
                     /locus_tag="N7A07_002784"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU2477416.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13511..13822)
                     /gene="ymdA"
                     /locus_tag="N7A07_002785"
     CDS             complement(13511..13822)
                     /gene="ymdA"
                     /locus_tag="N7A07_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU2477417.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13943..14275)
                     /gene="csgC"
                     /locus_tag="N7A07_002786"
     CDS             complement(13943..14275)
                     /gene="csgC"
                     /locus_tag="N7A07_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU2477418.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14334..14792)
                     /gene="csgA"
                     /locus_tag="N7A07_002787"
     CDS             complement(14334..14792)
                     /gene="csgA"
                     /locus_tag="N7A07_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU2477419.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(14833..15288)
                     /gene="csgB"
                     /locus_tag="N7A07_002788"
     CDS             complement(14833..15288)
                     /gene="csgB"
                     /locus_tag="N7A07_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU2477420.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3882 a         3952 c         3666 g         4009 t
ORIGIN      
        1 tattcctgct tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga
       61 agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt
      121 atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcaca aattcgtttt
      181 ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt
      241 ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac
      301 gctcagttat tacgatggct ggtatcacaa agcacccgaa ctacataaaa gcatcggtat
      361 tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg
      421 tccgctgccg agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt
      481 gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa
      541 accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca
      601 ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt
      661 tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg
      721 catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg
      781 tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat
      841 tgacaaagaa ggtcagcacg cctttgttaa ctttcgcatc cagcatcttg gctatagctg
      901 gttatatggc acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccagc
      961 tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaatc acgccgaacg
     1021 tgataaacat cttcgcagtg cagatttcct caataccgca aaatatccac aggcaacatt
     1081 cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct
     1141 gaatggtgta accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc
     1201 atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa attaagctca aggacttcaa
     1261 tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg
     1321 cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta
     1381 ttctgttgga tcaggaatgc ccagtgttgt attcagacgt ccacgagact tattaaagat
     1441 cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct
     1501 ttcttcgcag caaatttcac tacaacaacc tttgtatttt tccgcacata ctggacactg
     1561 aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcacc
     1621 gcactggtgg caatgcgcga taatctcatc agatatacgt tcgcccatcc gctcgtcaaa
     1681 aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta
     1741 ttcaataatt ccaccctcga tatgccagac tttattgaat ccgttatgtt tcatccaggc
     1801 actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg
     1861 tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc
     1921 cagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaacag
     1981 tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt
     2041 gctggcatca aagtgaggat cgtcaatacc gtcggcaacg atacgatcgc ggaccttcat
     2101 gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaagccatc
     2161 taaagccgag tcgaaggcat agagctgcgc gcgaaatgtt ttaacattgc tcgcaggtac
     2221 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
     2281 ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt
     2341 gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc
     2401 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
     2461 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca
     2521 tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc
     2581 acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg aataggttga
     2641 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
     2701 attggttaac ctggttgggt attggcgtac tttggttagt cgtgcaattg ccctacccgg
     2761 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
     2821 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
     2881 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg actgatggaa actggcatgg
     2941 cgtggttctg gccggaccgc cgaatcgctc gctggacgga agtgatcggc atggaacaca
     3001 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
     3061 tggagctggg tgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
     3121 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
     3181 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
     3241 ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtccct ttatttgccg
     3301 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc
     3361 tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc
     3421 cgccagagtg ttctccgccg ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag
     3481 tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta
     3541 aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccatgccgga tgcttcagaa
     3601 tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg
     3661 cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc
     3721 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
     3781 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggtgcc gccttcagtc
     3841 tggtaatgcc cttctaaccc ctctacgttg agcagcttgg cgttaccggc cactccgccc
     3901 tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac
     3961 cgttttgggg tggactcgcc gaccgtaaag gccgaaaact catgctatta cgctctgctc
     4021 tcggcatggg catcgtgatg gtgttgatgg ggctggcaca aaatatctgg cagtttttga
     4081 tcctgcgggc gcttcttggg ttacttggcg gatttgtccc caacgctaat gctcttatcg
     4141 ccacacaagt accgcgtaat aaaagcggct gggcactggg tacgctctcc acaggcggcg
     4201 ttagcggtgc gttgctcggc ccaatggctg gcggcctgct cgccgatagc tacggcttac
     4261 gtccggtatt ctttattacc gccagtgtgc tcatactctg ctttttcgtc accctgtttt
     4321 gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcacatg cgggaagtgg
     4381 tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcaccacg ttaatcatcc
     4441 aggtggcgac gggctcaatt gcccccattc tgacgctgta tgtccgcgaa ctggcgggta
     4501 acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc gtagctgctc
     4561 tgctgagtgc accacgactc ggcaaacttg gcgatcgaat cggacccgaa aagatcctga
     4621 ttacggcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt cagacgccat
     4681 tgcaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg
     4741 tacagacact gttggtttac aactcgagca accaaatcgc cggacgtatc ttcagctata
     4801 accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt gatgggagca gcgatttcag
     4861 cgaactacgg tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag
     4921 tctattcatg gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg
     4981 cctttcatac ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc tcatcaactc
     5041 aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc
     5101 gaagaatttc ttgcagacaa ccccggcatc gacgccgaag atgcgaatgt gcaacagttc
     5161 aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc
     5221 gacgaagggg aagaaggtga atgtttacct atgcttagcg gtgaagccgc gcaaagtgtt
     5281 tttgatggcg actatgatga gatagagatc cgccaggagt ggcaggaaga gaatacatta
     5341 catgaatggg acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc
     5401 gcagcagctg atgagtggga tgaacgttaa ttactcatac gggccatgat gaatgtcgat
     5461 cggcagcagt aaagtatcaa aaaccaggga gaatggcaga tcaaggattg tgacataacg
     5521 ccaggcggag tcacgcacat cccattgcac accgggataa tattggttgc catgcccctg
     5581 ccccggtata gtgcggctaa tgatgctgcc acagccactc agcaaggtca ccatgatgct
     5641 caccacaatt aaacgcattt gatacctctt ccgtaaaaaa ataccggcat aacgccggta
     5701 tttttattgg ctcgttgttt ttgccagatg tggcgtgaac gccttatccg acctacaaaa
     5761 tcttgcaaaa tcaacacatt gcaggagcga tgtaggcctg ataagcatag cgcatcaggc
     5821 aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga ttgagcttta
     5881 tcccttcgta ataactcacc catgaagaat atctctcagg ggaattccag acgcggaaat
     5941 gcagacgggc catcgtcacc ggatcgctta gcagcacaag gcgacgatcg cgattcagct
     6001 tctctggcgt ctcgttcagc gcctgttcaa catggcggtc acgggcgatt tccagcacct
     6061 tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat gacgggttaa
     6121 acactgcgtg cataaaacca tcatcaaggg agcgttgacg gttcatctca aggaaccgat
     6181 cggtatcaac cagtacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc
     6241 gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga
     6301 tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccaac cacgccatcc
     6361 cgacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac gcttcacccc
     6421 aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg
     6481 cgctgacgac gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaca
     6541 gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccatattct ttcgttcctt
     6601 tacaccaaat aagcaaaatg ctcaatagct ttggcaggaa caacagcacc atggtcgaag
     6661 caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac aactgccgtg
     6721 gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca gtagagagcg
     6781 cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaataccg
     6841 cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc aggttaccgt
     6901 ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggtaat tcttcataag
     6961 aacccgggag atcgtaagca atccagactc cccaacctgc acggcgcatc aacgccgctt
     7021 ccacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggggcca
     7081 gtgcacagtg ctcgataaac ggtttcacgc ggataatcgc gttatgcccc cagtagtgcg
     7141 actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg
     7201 cgaactgctg acagcgtgca tacagcgtat ccataccgga cgctttcggc gacgactgaa
     7261 tgatcccggc gttcgggttg gcttccatca ggcgcaccag gccgcacaaa caatcaccgg
     7321 tcattaccga gtcagcatcc agcaccacca tatagctgta ctggctgccc cagcgacggc
     7381 agaagtcatc gatattaccg cttttacgct tcacgcgacg gcggcggcgg cgatagaaaa
     7441 tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc
     7501 agatatccgg gttgtaactg tcactcagaa tgtagacatc aaagtgtttg gcattcccgg
     7561 tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc acgtcttcgt
     7621 tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg
     7681 ttgacgcaga tatactgtat ttatcgcgac caataagtag ttgcaggaag cccattaacg
     7741 ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc aggataccgg
     7801 tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga ccaaccatat
     7861 ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg
     7921 cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg gtaccgacgg
     7981 tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggtg
     8041 ttacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt
     8101 cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg cgcccttcgt
     8161 catctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct
     8221 ttaccgagcc ttgcggggag tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg
     8281 cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg
     8341 gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt
     8401 tcactcaacg tctgatcggc attcaccagc gcagcgcgca tttcagtggt tttcttggca
     8461 tctttcactt tcacacgcat caccagacgc cagcctttgg tgaccgggtt atagcgcacc
     8521 gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc
     8581 tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca
     8641 ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc
     8701 catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta
     8761 aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta
     8821 tcgttagttt catcgttggt tggaatttcc accagctcaa cgctgccttt accccactcc
     8881 cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag atcttcaaag
     8941 cgggagaaat cacgaccgcg ctgcaacaga ccaaagcctt gcgggttttc catggagaag
     9001 ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc gccattaccg
     9061 gcatggatag agagaccgtt agagtcgtgc aactccggac gatagttatt tgctggcgac
     9121 ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact
     9181 ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc tggcataact
     9241 acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt
     9301 ttatcagttg gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc
     9361 gatggcaagg cggtatcaat tgccagaccg cgggcagaaa ggccataaac ctgacctgca
     9421 ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg
     9481 ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg
     9541 tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg
     9601 gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac
     9661 tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc
     9721 tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct tttgggcgcc
     9781 tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc
     9841 cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc
     9901 atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc
     9961 cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat cgttcagcac
    10021 gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa
    10081 gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca
    10141 gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaactt gaagcctgcg
    10201 cctggcgcgg tcaggtcggt gtcgctatgg caacaagcct aaaggcataa cccgacaggg
    10261 ataagacgaa aaatccgaga ttagttaacc atatatgaat ccagtacccg cgcaacgtga
    10321 atatttcctc gactccatcc gcgcctggct gatgttgtta gggatccctt ttcatatttc
    10381 tttaatctat tcgagccata catggcatgt gaatagcgcc gaaccgtcat tgtggctgac
    10441 cctttttaat gacttcatcc actcgttccg catgcaggta tttttcgtta tatccgggta
    10501 cttttcctac atgctttttt tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga
    10561 acgtgtaggg atcccgatgt taacagccat ccccctactg acattgccgc aatttattat
    10621 gctgcaatac gtcaaaggga aagcggaaag ttggcctggg ctgtcattgt atgacaaata
    10681 taatacgttg gcctgggaat taatatcaca tctgtggttt ttactggtgt tagtggtcat
    10741 gacgacgctg tgcgtatgga tatttaaacg catcagaaat aatttagaaa attctgataa
    10801 aacgaataaa aaattctcga tggtaaaact atcggtgatt tttttgtgcc tcggcatcgg
    10861 ttatgcggta ataagaagaa cgatttttat tgtgtatcca cccattctga gtaatggcac
    10921 gttcaatttt attgtcatgc aaacgctatt ttatttaccg ttctttatcc tcggcgcact
    10981 ggctttcatt ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct
    11041 cgctgcagca ttggcgtttg tcgcttattt actcaaccag cgctatggca gtggcgatgc
    11101 ctggatgtac gaaaccgagt cggtgatcac catggtcctc ggtctgtgga tggtgaatgt
    11161 ggtcttctcc tttggccacc gtttgcttaa cttccagtca gcgcgggtga cttattttgt
    11221 taacgcatcg ctgtttatct atctggttca ccacccgtta acgctgtttt tcggcgcata
    11281 cattacaccg cacatcacct ccaactggct tggttttctc tgtggcctga tattcgtagt
    11341 agggattgcg ataattctgt atgaaattca tttgcgcatc ccgttactga agtttttgtt
    11401 ctctggtaaa ccggttgtta agcgtgagaa cgataaagca ccagcccgtt aagccacatt
    11461 tacaataacc attccacggg caatatcgac gccagtctga ccataacccg cttccagaaa
    11521 ctggtagcgg gttctttttt gagaacaatc tctttctttg catgacgatc aacccagttg
    11581 atccgtcccc acctgtccag acggagctgc caggccgcat catactggct ctgaataaag
    11641 cgtttatcaa ttaactgagc cagcgtttcg ctctctatca cgaagcccat ttcagtattg
    11701 agcaatgttg aacgcggatc gaaattgaaa gaaccgataa acaccgtttt accatcgatg
    11761 ctaaaggttt tagcatgcag gctggcaccg gaattaccgg ttatgccgcg atcgtgtaac
    11821 gtactacttt gttcacgcgt cggcttgagt tcatataatt ccacgccata gcggagcaat
    11881 tttttgcgcc agcgcgcgta tccggcatgg acgacagcaa catcgttagc ggcaagagaa
    11941 ttggttagga tcgcaatctt tacccctttt ctcaccatcc gtaagagttg cgccacacct
    12001 gcgcgtgtcg gtacaaaata ggaagagata atatcgatgc gttcactggg tgagcccatg
    12061 atatcgaaca ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc ccctttcgcc
    12121 ggatcatcac ttaataaacg tgttttcgcc cagataagcg gcaacgttcc atcaaccaga
    12181 tgatttataa acggactgga ttccattttg cgtaagtaac gatgcgtcat ggcatcgtta
    12241 tgccaggagg cgggtaactc gatgcgatcc gccatttcac cttccgggac atccagcacc
    12301 tgctgtaagg gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc
    12361 tctactacgg gtcctattgc catgacatct aaatccgaaa aaagtggctc ttcccctgcc
    12421 ccaaaatagg catcaccaat atttcgcccc cctaccaacg tcaccacgcc atcgacagtg
    12481 aaacttttat tgtgcatacg gcgattgaga cgggaaaagt cggtgatata accaagcgga
    12541 cgcagcaagc gaaacgagaa aggattaaaa agccggactt caatgcgggg atggctgtca
    12601 agcaagcgta aaatgtcgtc aagtccgggc gtattgttgt cgtccagcaa caaacggacg
    12661 cgaacgccac gctttgctgc ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg
    12721 tcctgccaga tgtaatactg aacatccagc gtatgctctg acatttcggc cagacgatac
    12781 cgggcggcaa acgcatccag acttttctcc agagggaaaa gaccacactg accgggatgt
    12841 tgcgaacaca gtggcagcac cgcgctcgcc agccggggca aatcattcat ctccttgttg
    12901 ggtaaggagt ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa agtatacctg
    12961 ttcgggtaaa gcgtgacggg taataaattc tgaaacggtt tttactgcga tttcagccgc
    13021 tgccgcacga gggtaaccat aaaccccagt actgattgca ggaaaagcca ctgacgtata
    13081 gctgtttgcc gccaccagtc gtaggctatt gagataggca tcctgcaaaa gctggtcttc
    13141 gttttgttca ccaccacgcc agactggccc gacggtgtgc actacggctt tagcaggaag
    13201 atcgcctgca agcgtaataa cggcatgccc cgtagggcaa tcgccctgct gttgcctgac
    13261 ttttaaacaa gcatccagca gggccggacc cgctgcgcga tgaatggccc catcgacgcc
    13321 gccacctccc attaatgaag gattagccgc attcacaatc acatcaacgg ccagtttggt
    13381 aatatcaccc tgcacaacat gaatacgcgt tttcataacg cctccttaca ccttcttgtt
    13441 tcttaagtgt acagcaggct ttttggttaa atcgcatttt tctgctcagc aacgtgagtt
    13501 gttcagaaac ttagttatat tcgatgttca ttacagccag ctttttctgt ttatcaagat
    13561 actgcacttt tactgaggct atttgcgcgt ttttgacatt gccgctcatt aacgctttgc
    13621 tggagtaggt tttacccgga acagaaccat tctgtgggca gttcatttct acgggtgatg
    13681 actgggtgct gacgtcacat gccggttcca caatttggcc ataaaaatgg atcacgcccc
    13741 cttgcgcggt gcttcctgcg atggcaataa aaggaaaaaa caaactgccg agcataagag
    13801 agtttaaaaa aggtctgaac atttattcta tttcctcaat gattagtcat ccttgagggt
    13861 tgtgttatcc atactttcag gttggctgat tcgcagcaga ccattctctc cagattcatc
    13921 ttatgctcga tatttcaaca aattaagact tttctgaaga gggcggccat tgttgtgata
    13981 aatgaagtga ctgtccatca gaaacagtaa caactatttt cacccgatcg tccggggaaa
    14041 tatttaaact caacttcgtc aaagcaatgg gttgattagc aggcaatgaa agggtctttt
    14101 cttgcttcgt ctgactttgc cctgaactgc cttcgcgcag ggacaatatt tgtactctgc
    14161 acagacaaga ttgagtaaga gtgacttcag gaataatggt gtacatatcc ccttgctggg
    14221 tcgtattaaa ggttatctga ctggaaagtg ccgcaaggag taataacgca ttcatattct
    14281 tctcccgaaa aaaaacaggg cttgcgccct gtttctttaa tacagatgat gtattagtac
    14341 tgatgagcgg tcgcgttgtt accaaagcca acctgagtta cgttgacggt ggaattagat
    14401 gcagtctggt caaccgctgc accgttgccg ccaccgaatt gtttaactgt catatgagag
    14461 tctttaccgt tccactgatc aagagtggcg ctgttaccaa agccacgttg ggtcagatcg
    14521 attgagctgt catctgagcc ctgaccaaca tctgcaccgt taccaccacc atgctgggta
    14581 atagtaagat cagagttacg agcatcagct tgcagagcaa gtgcagagtt accaccaccg
    14641 tactgataaa tattcagctc tgaattcggg ccgctgttat taccgccacc accgtggtta
    14701 ccgccaccgc cgccgtactg aggaacaaca cctgccagag cgctaccgga gaatacgatt
    14761 gctgcaattg ctgctacttt taaaagtttc atgtaaaacc cccatcggat tgatttaaaa
    14821 gtcgaatgga aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc tctgcactac
    14881 aattgccgtt ttttgagtac catactgtgt aatatttgct ttattaccag aacctttctg
    14941 gataatcatc gcagtattac cataagcacc ttgcgaaata ctggcatcat tggcactgcc
    15001 cgcctgatca atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccggttgct
    15061 actaccttct tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact
    15121 attattagtc ccagcttgac caattatggc tgcctgatta aatgaagact tactcaattc
    15181 atttaccgcg aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc
    15241 aggcgcaccc agtattgtta acatcataaa taacaatttg tttttcatgt tgtcaccctg
    15301 gacctggtcg tacatttaag aaattaaatc atttcaactt ggttgttaac gcaacctata
    15361 ttttgttaac gctgcgttac gatggaaagt atgtctgcgg aaatattttt aataactcac
    15421 ccgcgcgttg tatttttctt tttttgttca taccaaagta ttaaaaccca cgtaaatacg
    15481 ctgttatcta cgcaaagaat attttgttt
//
LOCUS       DBFIFS010000102        15460 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.102, whole genome shotgun sequence.
ACCESSION   DBFIFS010000102 DBFIFS010000000
VERSION     DBFIFS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.102"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..782
                     /gene="mhpR"
                     /locus_tag="N7A07_002789"
     CDS             <1..782
                     /gene="mhpR"
                     /locus_tag="N7A07_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU2477421.1"
                     /translation="LMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVR
                     RSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVV
                     RETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDDYQL
                     AREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAM
                     TIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            897..1673
                     /locus_tag="N7A07_002790"
     CDS             897..1673
                     /locus_tag="N7A07_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGU2477422.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            1693..2646
                     /locus_tag="N7A07_002791"
     CDS             1693..2646
                     /locus_tag="N7A07_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HGU2477423.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            2683..3765
                     /gene="lacI"
                     /locus_tag="N7A07_002792"
     CDS             2683..3765
                     /gene="lacI"
                     /locus_tag="N7A07_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU2477424.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            3888..6962
                     /gene="lacZ"
                     /locus_tag="N7A07_002793"
     CDS             3888..6962
                     /gene="lacZ"
                     /locus_tag="N7A07_002793"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU2477425.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFHHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            7014..8267
                     /gene="lacY"
                     /locus_tag="N7A07_002794"
     CDS             7014..8267
                     /gene="lacY"
                     /locus_tag="N7A07_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU2477426.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            8333..8944
                     /gene="lacA"
                     /locus_tag="N7A07_002795"
     CDS             8333..8944
                     /gene="lacA"
                     /locus_tag="N7A07_002795"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU2477427.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(9047..10228)
                     /gene="cynX"
                     /locus_tag="N7A07_002796"
     CDS             complement(9047..10228)
                     /gene="cynX"
                     /locus_tag="N7A07_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU2477428.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSIRGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(10234..10704)
                     /gene="cynS"
                     /locus_tag="N7A07_002797"
     CDS             complement(10234..10704)
                     /gene="cynS"
                     /locus_tag="N7A07_002797"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU2477429.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(10735..11394)
                     /gene="cynT"
                     /locus_tag="N7A07_002798"
     CDS             complement(10735..11394)
                     /gene="cynT"
                     /locus_tag="N7A07_002798"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU2477430.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            11504..12403
                     /gene="cynR"
                     /locus_tag="N7A07_002799"
     CDS             11504..12403
                     /gene="cynR"
                     /locus_tag="N7A07_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU2477431.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(12536..13819)
                     /gene="codA"
                     /locus_tag="N7A07_002800"
     CDS             complement(12536..13819)
                     /gene="codA"
                     /locus_tag="N7A07_002800"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU2477432.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(13809..15068)
                     /gene="codB"
                     /locus_tag="N7A07_002801"
     CDS             complement(13809..15068)
                     /gene="codB"
                     /locus_tag="N7A07_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU2477433.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         3728 a         4104 c         4100 g         3528 t
ORIGIN      
        1 gtctaatgtt attaaatatg ttaaataaac ttgacggcgg tgccagcgtc gggctgctgg
       61 cggaactcag cggcctgcat cgcaccactg tgcggcgact gctggagacg ctgcaggaag
      121 agggatatgt ccggcgtagt ccctccgatg atagttttcg tctgaccatc aaagtgcggc
      181 aattaagcga aggatttcgt gacgaacagt ggatttctgc actggcggcc ccactgctgg
      241 gcgatctgtt gcgcgaagtg gtatggccga cagatgtgtc cacgctggat gttgatgcaa
      301 tggtggtacg cgaaaccact caccgtttca gccgcttatc ctttcaccgg gcaatggtcg
      361 ggcggcgttt accgcttctg aaaaccgcct cgggcctgac ctggctggcc ttttgcccgg
      421 aacaagaacg caaggaatta atcgagatgt tagcctcccg ccccggtgat gactatcaac
      481 tggcacgaga accgttaaag ctggaagcca ttctggcgcg cgcgcgcaaa gagggttacg
      541 gacagaacta ccgcggctgg gatcaggagg agaagatcgc ctctatcgcc gtaccgctgc
      601 gcagtgaaca acgggtgatt ggctgtctga atctggtgta tatggcgagc gcaatgacca
      661 tcgaacaggc agcggaaaag catcttccgg cactacaacg ggtagcaaaa cagatcgaag
      721 aaggggttga atcgcaggct attctggtgg ccggacggcg aagcggcgtg catttacgct
      781 agttacgcca atgtgtaatt tggcccattc tcatcgttat tcgcccattt gcccacatgc
      841 acagtacggg ctgcacgcta cgctaatgac atcaatactg tgatgtggag caaatcatga
      901 aaattacgca aattcgtaat gccacccaaa ttatccaata cgctggcaaa acatttctta
      961 tcgacccgtt actcgcgcct aaaggaagtt atccgggttt tgccggaacg gcacgcgcag
     1021 aaatccgcaa tccaacggta gaattaccgt gcgatttaac aacgttactc aacgttgacg
     1081 ccctaatcgt gacccatttg catgaagatc actgggacga aacagcctcc cgcgtcgtcc
     1141 ctaaagataa actgatttat gtgcaaaatg agcaagatgc tgaatcactg aataaacaag
     1201 gatttacgca tcttaccgta cttaccgacg agactaaatt tggcgatatt actctgcata
     1261 aaacgacagg gcagcacggc tccgatcgca cctatgccga cccggcgatg gcgaagcgtt
     1321 taggcgcagc ttgcggcgtt gttttccagc acccgacaga gaaaacgctc tatctggctg
     1381 gcgataccct ctggcgtgac gacgtcgagg ctacaatgct aactttccaa ccagaagtgg
     1441 ttgtgttgaa tgccggattt gcgcatgttc tggggcatgg cgcgataatt atgggagcag
     1501 aagatattct caaaacacat tttaccctcc cagaagcgca aattatcgcg acacatatgg
     1561 aggccgtaaa ccactgtttg ctgacgcgtg atgcgctcaa ggaatacgcc agagataatc
     1621 aaattatcga atttgtgaac gtaccagaag atggtgaaac gttgacattc taattcggag
     1681 ataaagagtg ttatgcgccc gctaactgtt gccattatcg ccgttgccgg ttttagtcct
     1741 tttcaccttt ccgtaccgtt tatcgtgttt agtgaaaaga tggcggagaa aaaacgcttt
     1801 cacgtgatta tttgcgctga aaagccggga aacgtggact ctgccgatgg gttttccgta
     1861 accgccaccc atgactatac cgcagtcatc caggcagata ttgtgataat tccttactgg
     1921 ggaaccatta cacaaaaacc gccacaaaaa ctgctggaag ccttaacgac cgcacgggat
     1981 aacggcgcac agattgtcgg gctttgcctg ggcacgtttg tgctcggcta tgcaggttta
     2041 ctgaaaaata agcgtgccgc cacgcactgg gagttcgagc gtgaatttca ggcacgtttt
     2101 ccacaaacac atctggatat taacgcgttg tacgtagacg atgacggcat tattacctct
     2161 gccggtactg ccgcggcgct ggattgctgt ttgtatattg ttcggcaaca ttttggcagc
     2221 gactatgcta accatattgc ccgacggatg gtcgtaccgc catatcgcac cggcggtcag
     2281 gcgcagttta ttgagcaacc ggtgccgaaa aatacccatg atgaacgcat aaacctcctg
     2341 ctggattacc tgcggcaaaa cattgcgcaa cagcatgatc tcgactcgct ggcgcagcga
     2401 gtaatgatga gtcgccgcac attaactcgc cattttatga aagcgaccgg ttcgagtatc
     2461 gccgaatggc tcattactga acgcttacgc cgtagccagg aactgttggg atccagtcag
     2521 ttgcccgttg agcggatagc ggctgaggtg ggttttctct cacctgtgac ctggcgtcag
     2581 cattttaaat ctcacttcgg cgtcagcccc gccgaatggc gcaaaacctt tcgcggtatg
     2641 gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgcta
     2701 tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaatcag
     2761 gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat
     2821 tacattccca accgcgtggc acaacaactg gcggggaaac agtcgttgct gattggcgtt
     2881 gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
     2941 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
     3001 tgtaaagcgg cagtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
     3061 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcgctaatgt tccggcgtta
     3121 tttcttgatg tctctgacca gactcccatc aacagtatta ttttctccca tgaagacggt
     3181 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
     3241 ggcccattaa gttctgtctc ggcgcgtctg cgtctggcgg gctggcataa atatctcact
     3301 cgcaatcaaa ttcagccgat agcggaacgg gagggcgact ggagtgccat gtccggtttt
     3361 caacaaacca tgcaaatgct aaatgagggc atcgttccca ctgcgatgct ggttgccaac
     3421 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggttgcg cgttggtgcg
     3481 gatatctcgg tagtgggata cgacgatacc gaagacagct cgtgttatat cccgccgtta
     3541 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     3601 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     3661 aaaaccaccc tggcgcccaa gacgcaaacc gcttctcccc gcgcgttggc cgattcatta
     3721 atgcagctgg cacgacaagt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3781 tgtgagtcag ctcactcatt aggcacccca cgctttacac tctatgcgtc cggctcgtat
     3841 gttgtgtgaa attgtgagcg gataacaatt tcacacagga tacagctatg actatgatta
     3901 cagattcact ggccgtcgta ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3961 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4021 ccaatcgccc ttcccagcag ttgcgcagcc tgaatggtga gtggcaattt gtctggtttc
     4081 cggcaccaga agcggttccg gaaagctggc tggagtgcga tcttcctgac gccgatactg
     4141 tcgtcgtccc ctcaaactgg cagatgcacg gttacgacgc gcccatctac accaacgtga
     4201 catatcccat tacggtcaat ccgccatttg ttcccacgga gaatccgacg ggttgttact
     4261 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     4321 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     4381 acagtcgttt gctgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
     4441 tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc
     4501 ggatgagcgg cattttccgt gacgtctcgt tgctgcacaa accgaccaca caaatcagcg
     4561 atttccatgt tgccactctc tttaatgatg attttagccg cgcggtactg gaggcagaag
     4621 ttcagatgta cggcgagctg cgcgatgagc tgcgggtgac ggtttctttg tggcagggtg
     4681 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggcg
     4741 gttatgccga tcgcgtcaca ctaggtctga acgtcgaaaa cccgaaactg tggagcgccg
     4801 aaatcccgaa tatctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
     4861 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
     4921 tgaacggcaa gccgttgctg attcgcggcg ttaaccgtca cgagcatcat cctctgcatg
     4981 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctaatgaag cagaacaact
     5041 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacc ctgtgcgacc
     5101 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
     5161 atcgtctgac cgatgatccg cgctggttac cggccatgag cgaacgagta acacgaatgg
     5221 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gagtcaggcc
     5281 acggcgctaa tcacgacgca ctctatcgct ggattaaatc tgtcgatcca tcccgcccgg
     5341 tgcagtatga aggcggcgga gccgacacct ccgcaaccga tattatttgc ccgatgtacg
     5401 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
     5461 tttcgctgcc tggagaaatg cgcccactga tcctttgcga atacgcccac gcgatgggta
     5521 acagtcttgg cggcttcgct aaatactggc aggcgtttcg tcagtacccc cgtttacagg
     5581 gcggcttcgt ctgggacttg gtggatcagt cgctgattaa atatgatgaa aatggcaatc
     5641 cgtggtcggc ttacggcggt gattttggcg atacgccgaa tgatcgccag ttctgcatga
     5701 acggtctggt ctttgccgac cgcacgccgc atccggcgct gacggaagca aaacaccagc
     5761 agcagttttt ccagttccgt ttatccgggc gaaccatcga agtgaccagc gaatacctgt
     5821 tccatcatag cgataacgag ctcctgcact ggacggtggc gctggatggt aagccgctgg
     5881 caagcggtga agtgcctctg gatgtcgctc cgcaaggtaa acaggtaatt gaattgcctg
     5941 aactaccgcg actggagagc accgggcaac tctggctaac ggtacacgta gtgcaaccga
     6001 acgcgaccgc atggtcagaa gccggacaca tcagcgcctg gcagcagtgg cgtctggcgg
     6061 aaaacctcag cgtgacactc ccctccgcgc cccacgccat cccgcaactg accaccagcg
     6121 aaacggattt ttgcatcgag ctggataata agcgttggca atttaaccgc cagtcaggct
     6181 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
     6241 tcacccgcgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
     6301 acgcctgggt cgaacgctgg aaggctgcgg gccattacca ggcagaagcg gcgttgttgc
     6361 agtgcacggc agatacactt gccgacgcgg tgctgattac cactgtccac gcatggcagc
     6421 atcagggaaa aaccttattt attagccgga aaacctaccg gattgatggt agtggtcaaa
     6481 tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccggca cggattggcc
     6541 tgacctgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     6601 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     6661 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     6721 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagcc
     6781 aacaacaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggaacatggc
     6841 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     6901 cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     6961 aataataacc gggcaggcca tgtctgcccg tatttcgtgt aaggaaatcc attatgtact
     7021 atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca
     7081 tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat atcagcaaaa
     7141 gtgatacggg tattattttt gctgctattt ctctgttctc gctattattc caaccgctgt
     7201 ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt attaccggca
     7261 tgttagtgat gtttgcgccg ttctttattt ttatcttcgg gccactgtta caatacaaca
     7321 ttttagtagg atcgattgtt ggtggtattt atcttggctt ttgttttaac gccggtgcgc
     7381 ccgcagtaga ggcatttatc gagaaagtca gccgtcgcag taatttcgaa tttggtcgcg
     7441 cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc gattgtcggc atcatgttca
     7501 ccatcaataa tcagttcgtt ttctggctgg gttctggctg tgcactcatc ctcgccattt
     7561 tactcttttt cgccaaaacg gatgcgccct cttccgccac ggttgccaat gcggtaggtg
     7621 ccaaccattc ggcatttagc cttaaactgg cgctggaact gttcagacag ccaaaactgt
     7681 ggtttttgtc actgtatgtt attggcgttt cctgcaccta cgatgttttt gaccaacagt
     7741 ttgctaattt ctttacttct ttctttgcca ccggtgaaca gggtacgcgg gtatttggct
     7801 acgtaacgac aatgggcgaa ttacttaacg cctcaattat gttctttgcg ccactgatca
     7861 ttaatcgcat cggtgggaaa aatgccctgc tgctggctgg cactattatg tctgtacgta
     7921 ttattggctc atcgttcgcc acctcagcgc tggaagtggt tattctgaaa acgctgcata
     7981 tgtttgaagt accgttcctg ctggtgggct gctttaaata tattaccagc cagtttgaag
     8041 tgcgtttttc agcgacgatt tatctggtct gtttctgctt ctttaagcaa ctggcgatga
     8101 tttttatgtc tgtactagcg ggtaatatgt atgaaagcat cggtttccag ggcgcttatc
     8161 tggtgctggg tctggtggcg ctgggcttca ccttaatttc cgtgttcacg cttagcggcc
     8221 ccggcccgct ttctctactg cgtcgtcagg tgaatgaagt cgcttaagca atcaatgtcg
     8281 gatgcggcgc gagcgcctta tccgaccaac atatcataac ggaatgatcg caatgaacat
     8341 gtcgatgaca gaaagaataa aagcaggcaa gctatttacc gatatgtgcg aaggcttacc
     8401 tgaaaaaaga cttcgtagaa aaacgttaat gtatgagttt aatcactcgc atccatcaga
     8461 agttgataaa agagaaagcc tgattaaaga aatgtttgcc acggtagggg aaaacgcctg
     8521 ggtagaaccc cccgtctatt tctcttacgg ttccaacatc catataggcc gcaattttta
     8581 tgcaaatttc aatttaacca ttgtcgatga ctacacggta acaatcggtg ataacgtatt
     8641 gattgcaccc aacgttactc tttccgttac gggacaccct gtacaccatg aattgagaaa
     8701 aaacggtgag atgtactctt ttccgataac gattggcaat aacgtctgga tcggaagtca
     8761 tgtggttatt aatccaggcg tcaccattgg ggataattct gttattggcg cgggtagtat
     8821 cgtcacaaaa gacattccac caaacgtcgt ggcggctggc gttccttgtc gggttattcg
     8881 cgaaataaac gaccgggata agcaatatta tttcagagat tacaaagttg aatcgtcagt
     8941 ttaaattata aaaattgcct gatacgcttc gcttatcagg cctacaaatt cagtgatcta
     9001 tgtaggccgc atccggcgtg aacaaagcgc agaaacaagc gtcgcatcat gcctctttga
     9061 cccacagctg cggaaaacgt gctggtgcaa aacgcagggt tataatcatc agccctacga
     9121 cgcacagcgc atgaaacgcc cagtccatca agtaattgcc cctgatacta cgcagcacgc
     9181 cagaaaacca cggggcaagc ccggcgatga taaaaccgat tccctgcata aacgccacca
     9241 gcttgccagc aatagccggt tgcacagagt gatcgagcgc cagcagcaaa cagagcggaa
     9301 atgcgccgcc cagacctaaa ccacacacca tcgcccacaa taccggcaat tgcagcggca
     9361 gccagataaa gccgcagaaa cccaccagtt gtaacgccag cgccagcatt aacagtttgc
     9421 gccgatcctg atggcgagcc atagcaggca tcagcaaagc tcctgcggct tgcccaagcg
     9481 tcatcaatgc cagcaaggaa ccgctgtact gcgcgctggc accaatctca atatagaagg
     9541 cgggtaacca ggcaatcagg ctggcgtaac caccgttaat cagaccgaag taaacaccca
     9601 gcgtccacgc gcgaggagtg aaaaccacgc gaaccggaat ggttgttgtc ttgtgggaag
     9661 aggcgacctc gcgggtgctt tgccaccacc aggcaaagag cgcaacaacg gcaggcagcg
     9721 cccaccaggc gagtgtttga taccaggttt cgctatgttg aactaaccag ggtgtcaggg
     9781 cggcaccaag cccaccgccg cccatcagag ccgcggacca cagccccatc accagtggcg
     9841 tgcgctgctg aaaccgccgt ttaatcaccg aaggcatcac cgcctgaatg atgccgatcc
     9901 ccaccccacc gagcagtgcg ctgctaagca gcagcgcact ttgagggtaa agctcacgca
     9961 tcaatgcacc gacggcaatc agtaacaaac tgatggcgac gctgcgacgt tcgctgacat
    10021 gctgatgaag ccagcttccg gccagcgcca gcccgcccat ggtaaccacc ggcagagcgg
    10081 tcaacagggc agccacgcta aagctcattc cgcttgcctg gcgcaattgc ggtagcagtg
    10141 gcccgacgga ggtgagcagt ggtcgcatat taagaccaat cagcaccaga accagcagca
    10201 tcgtgaacgt cttttgatgg ttacgcatgg ctgtcagaac ggtttggtcg gcagatattt
    10261 accatctaag gtgatgaccg cacgttcgcc accttccggg tccgccactt tcttaacgtc
    10321 gagtttgaag ttaatcgcgc tgataatgcc atcgccaaat ttctcatgaa ccaacgcttt
    10381 cagggttgtg ccgtacacct gcaacatttc atagaaacga tacatcgttg ggtcggttgg
    10441 aatacggtca tcaatgcagc cacgcagtgg gatcatctgt aacagtagaa tggcgtcttc
    10501 gtcgagatcc agcttcgccc cgacctggcg ggcggcgtcg gcaggaagcg cctgctgacc
    10561 cagcaaagcc gcggtcacaa aggcttctgc cagcccggtg ccgtcggcaa tctcggcaaa
    10621 tgagagatct tttttagctt tgctgagcaa aatggcatcg gcaagatcaa gacgaatatt
    10681 gcggttaatt tgcgactgaa tcatggtgca tctcctgatg gtttaaaaat aaggttacgc
    10741 tgcggtcggt tggcgtagcg gtatggcaca aacgcgagga ttagctgcca gtggcacaaa
    10801 ctggcgggtt gcgccgtcaa acgctgcgat gctgccgctt tcaatgtcgt agacccagcc
    10861 gtgcagggcg atccgcccct cttcgagcgc caggcgcacc gatggatgag tttgcaaatt
    10921 agccaactga gcaatgacgt tttcacgtac catcgccgca gcttttgacg gtaaatcgga
    10981 atgcgggcgc gcctcattaa cgacgcgggc tgaatcggca taacgcagcc agtgggagac
    11041 ggcaggcata tggtccatgc actgacagct ggcaatggcg gtcatcgcgc cacagttgga
    11101 atgaccgcaa atcacaatgt cagatacccg aagcgcagcg acggcatact ccaccgaagc
    11161 agaaacgcca ccgggttccg gcccgtagga agggacgata ttgcccgcat tgcgaataac
    11221 gaaaagatcg ccaggctcac gttgcgtcac cagctcaggg accagacggc tgtcggagca
    11281 ggagataaaa agtgtgcgcg ggctttgctg tgtcgccaac tgtttaaata aggcttcccg
    11341 cttcggaaat gcctcgcgct ggaatttaag gaatccatca ataatctctt tcaccgttaa
    11401 cctctatctg tctctggaat gagaggcaga ctactcgccg attgtcataa ggtaaaagtc
    11461 tcatttatga tgagttctat tgaatttact tataggttgg cgaatgctct ctcgacatat
    11521 caattatttt cttgccgtgg ctgaacatgg cagcttcacc cgtgccgcca gtgcgttgca
    11581 cgtctctcag cctgcgcttt cccagcagat tcgccagtta gaggagagtt taggcgtgcc
    11641 gctgtttgac cgtagcgggc gaacgattca tctcactgat gcaggagaag tctggcgaca
    11701 gtacgccagc cgggcgttac aggaactggg agcgggtaaa cgggcgattc atgatgttgc
    11761 cgatctgacg agaggatcgc tgcgtatcgc cgtcaccccc acctttacga gctactttat
    11821 cggcccctta atggcggatt tctatgcgcg ctaccccggc atcacgctcc agctacagga
    11881 aatgtcgcag gagaaaatcg aagatctgct ttgtcgcgac gagctggacg ttgggattgc
    11941 cttcgcgcct gtgcatttgc cggagctgga ggcaattcct ttactgacag aaagtttagc
    12001 gttagtcgtg gcgcaacatc atccgctggc tgcctgtgaa caggtggcgt tgagtcgctt
    12061 gcatgatgaa aaactggtcc tgctcagcgc ggaatttgcc accagagagc agattgacca
    12121 ctactgcgag aaagcggggc tgcatccaca ggtggtcatt gaggcgaact caattagcgc
    12181 ggttctggag ctgattcgcc gcacttccct ttccacattg ttaccagcag cgattgccac
    12241 acaacatgac gggcttaaag cgattcctct tgccccgcca ctactggaga gaacggcggt
    12301 tttgttgcgg cggaaaaata gctggcagac agccgccgcg aaggcatttt tgcacatggc
    12361 gttggaggaa tgtgcggatg ttggcgagaa tgaatcgcgg taaccactga cacaattgcc
    12421 tgatgcgtta cgcttatcag gcctacgagt tcagtgctct acgtaggcct gataaggcgt
    12481 tcaagccgca tccggcataa actacgctcg ctttgtcaac acacagccca gccgatcacc
    12541 gtttgtaatc gatggcttcc ggctgctcca gatatacggt ggtttgtgcc ggttgtgtgc
    12601 tggcaatcac cttgccgcca cgtaccgaat aacgtaccgg aacctgacgg cgcagcgcat
    12661 caaatccatt ttcagccggc aggataatca ggttggcgct gtttccggcg gcaatgctgt
    12721 aatcctgcaa attcaacgtc ctggcgctgt ggtgggtgat taaattcagg ccatcgttaa
    12781 tctgcccata gcccatcagc tggcaaacat gcagccccat atgcagcact tgcagcatat
    12841 tcgccgttcc cagcggatac cacggatcga agacatcatc gtgaccaaag cagacgttaa
    12901 tgccggactc cagcatctct ttaacgcgcg tgatgccgcg acgttttgga tacgtatcga
    12961 atcgtccttg cagatgaata ttgaccagcg ggttggcgac aaagttaata ccggacattt
    13021 tcagcaagcg gaacagacgt gaggtatacg ccccgttata agagtgcatt gccgtggtgt
    13081 ggctggcggt gactcgcgcg cccatgcctt cacggtgcgc cagggcagca acggtttcga
    13141 caaagcgcga ctgctcgtca tcgatctcat cacagtgaac gtcgatgaga cggtcgtatt
    13201 tttgcgccag ggcgaaggtt ttatgcagcg actccacgcc gtattcacgg gtaaattcaa
    13261 aatgcggaat cgcccccact acatctgccc ctaagcgtaa cgcctcttcc agcaacgctt
    13321 caccgttggg atacgacaaa atcccttcct gagggaaggc gacgatttgc agatcaatcc
    13381 acggcgcgac ttccagcttc acttccagca ttgctttcag cgcagttagc gttgcatccg
    13441 aaacatcgac atgggtacgc acatgctgaa tgccgttggc aatctgccat ttcagcgttt
    13501 gccatgcgcg ttgtttcaca tcgtcatggg ttaataacgc tttgcgctcg gcccagcgtt
    13561 caatgccttc aaacagcgtg ccggactgat tccagttcgg ttgtccggcg gtttgcgtgg
    13621 tgtccaggtg aatatgtggc tccacaaacg gcggtataac taaaccttgt tcggcatcca
    13681 ggctgttttc agttatgggc atcacgccgg attgcgcatc aatggcgctg atttttccgt
    13741 cctgcagatg aatctgccac agcccctctt tgcctggcaa ccgggcgtta ataattgttt
    13801 gtaaagcgtt attcgacact gttagcctcc acatgcgtca ttgctgctgt cgttttacga
    13861 ttcaaaatcg ggttaaggat cagatagctc agcgcgccac ctaataccgc gttgaccgga
    13921 acaattcccg gtaaccagtg gcctgcagca atccccaggg cgaccgccag aatcgccacc
    13981 caattgacac tcatcatacg cgtggtcgca aagtgctcat agcggcgacg gttcatcaga
    14041 tagtcggcaa tgatcacgcc acccactgga ggaatagctg ccgaaaggaa ggtcaaccag
    14101 ccgacaaaat tgttatacag ccataatgca cagaccgtac cgataatacc gttgattacc
    14161 gaaagggttt tgctcgacat cccggtaatg ttggcgaaac ctaaacccga cgcatagagc
    14221 gcgttatcgt tggtggtcca gatatttagc cccagcacca caatcgcagg caacagcagg
    14281 ccctgagcaa tcatcacatc agagatatcc gccatcccca gtgccgcagc gcccgctgca
    14341 ccgaaaataa acatcaacga gttgccgagg aaaaaggcca ccatcgccac cagcaccgcc
    14401 agtttggcat tgcgaccaaa gcggacaaag tcagcggtga gcgtgcccgc actgataaat
    14461 gaccccacaa ccagcgccag cgcgacattg aaatctaacg gttgtgcggg aacgaccgct
    14521 tttaaggcgt ccaggccgcc catgccgtta acggccagcc acacggaata accgccaaga
    14581 caggcgatag ccggaaccgc aatcaacgaa agaaccgtca gcgccgaaat gccaaagaag
    14641 acggtgacgg tcatcagtaa accggaaacg gcaatcagca aattaatatc cagcccggtt
    14701 gccttgccca ccggaatagc aaacatcgcc acaccgacgc caaaccagcc aacctgggtg
    14761 ccgcccagca gcagtgaagg cagccatgag cctttaacac caaacgagaa gcgagcaaga
    14821 agatgagtgg tcaggccggt ttttgcgcca atgtaaccga gaaatgaagt gtaaataccg
    14881 aggagaagat taccgatgag aactgcgagg aagaaatcat gatagctaag accggttccg
    14941 agagtgccgc cggtccacat actggcggaa aagaaggtta atcccagcat gacgaacgtc
    15001 aatgccaata cccctttccg cgccgactgc gggaccggcc cctggctaaa gttgttatct
    15061 tgcgacacga aattccccct ttttgctgtt tgaaacccaa aaaatccgcc gcattctatt
    15121 catctgaaga taaaaagcaa tcgttttcgt ggggaaatat attttttata tgaatgagtg
    15181 caaaaaggtg gggggaaaat tatcggtgta aaaacagtgg gtaggcatga taagacgcgt
    15241 cagcgtcgca tcaggcaact acgcacggtg tcggatgcgg cgcgagcgcc ttatccgacc
    15301 tacggttcag gcctgtaggc atgataagac gcgcctgcgt cgtatcaggc atctgcgcac
    15361 ggtgtcggat gcggcgcgag cgccttatcc gacctacggt tcaggcctgt aggcatgata
    15421 agacgcatca gcgtcgcatc aggcactgag cacggtgtcg
//
LOCUS       DBFIFS010000103        15452 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.103, whole genome shotgun sequence.
ACCESSION   DBFIFS010000103 DBFIFS010000000
VERSION     DBFIFS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.103"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            125..328
                     /locus_tag="N7A07_002802"
     CDS             125..328
                     /locus_tag="N7A07_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU2477434.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1407
                     /locus_tag="N7A07_002803"
     CDS             328..1407
                     /locus_tag="N7A07_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2477435.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSVWKPTSRESVTLTAIEDGLAA
                     LWKKYEETVNHRDRAMTFGRLWEKFLASAYYSELSPRTQKDYLQHQKKLLAVFGKVLA
                     DSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAKN
                     RERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGKT
                     GKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNHAGKISG
                     ILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPENI
                     PRKYSKKIFQVIPSVIFVTDLMMCK"
     gene            complement(1416..1505)
                     /locus_tag="N7A07_002804"
     tRNA            complement(1416..1505)
                     /locus_tag="N7A07_002804"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Ser,seq:cga)
     gene            1599..2396
                     /gene="mtfA"
                     /locus_tag="N7A07_002805"
     CDS             1599..2396
                     /gene="mtfA"
                     /locus_tag="N7A07_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU2477436.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2497..2572
                     /locus_tag="N7A07_002806"
     tRNA            2497..2572
                     /locus_tag="N7A07_002806"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2530..2532,aa:Asn,seq:gtt)
     gene            2886..10868
                     /locus_tag="N7A07_002807"
     CDS             2886..10868
                     /locus_tag="N7A07_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU2477437.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQIVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11130..12182)
                     /locus_tag="N7A07_002808"
     CDS             complement(11130..12182)
                     /locus_tag="N7A07_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU2477438.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12496..13812
                     /gene="shiA"
                     /locus_tag="N7A07_002809"
     CDS             12496..13812
                     /gene="shiA"
                     /locus_tag="N7A07_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU2477439.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13914..15368
                     /gene="amn"
                     /locus_tag="N7A07_002810"
     CDS             13914..15368
                     /gene="amn"
                     /locus_tag="N7A07_002810"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU2477440.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4233 a         3678 c         3707 g         3834 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagaaa atattccaag taattccaag tgtgattttt
     1381 gtcactgact taatgatgtg taagtgattg aattttggcg gagagagggg gatttgaacc
     1441 cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg ctccggcatc
     1501 tctccgttca gatggttgcc atgatgccag gaaatttggc attttaacag tccctgtccg
     1561 tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg ccctggaaag
     1621 tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg atcccccttt
     1681 taacgtgtct gacggaacag gaacaaagca aattagtcac tcttgccgaa cgttttttac
     1741 agcaaaagcg gcttgttcct ttacagggct ttgaactgga ttcattaaga agctgccgga
     1801 tagcacttct attttgccta cccgttctgg agttaggact ggaatggctg gatggttttc
     1861 atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa gacgatatcg
     1921 gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa gggcctatcg
     1981 ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac ctgattattc
     2041 atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga gttcccttta
     2101 ttccgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg aacaacattc
     2161 aggaagaaat cgaattggtt ggcgagaatg cggcgagcat tgatgcttat gctgccagtg
     2221 atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca gaactttttg
     2281 ctcctcgttt cccttcattg tggcaacgtt tctgccaatt ttatcaacaa gatcctttgc
     2341 agagactgca tcacgctaat gatacagact cgttttcggc gacgaatgtt cattaattaa
     2401 caactttgca gattaattaa ccaattgaaa tgacttatga aatttagtgt tgacagacaa
     2461 ggtaccgcta agtaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa
     2521 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattctaa
     2581 aaattcgctt ttttagcgca atgtcactga ccttagttga acattgtttt ttaacggata
     2641 gcgggttttt aacatcttaa gcgccctcga cctttatggt tgagggcgtt ttgctatgaa
     2701 cgccatcacc attttcccct cgattataaa acttgagtta ttcagtagtc tcccctcttg
     2761 caactcacat ccaaaactgc ctaacgaaaa gttattaatt ttcaatcata ttgctatcag
     2821 gatttacatt ttttcgctgt gctagaaagg gcgcatttat gttagctcgt tcagggaagg
     2881 taagcatggc tacgaagaag agaagtggag aagaaataaa tgaccgacaa atcttatgcg
     2941 ggatgggaat taaactacgc cgcttaactg cgggtatctg cctgataact caacttgtgt
     3001 tccctatggc tgcggcagca caaggtgtgg taaacgccgc aacccaacaa ccagttcctg
     3061 cacaaattgc cattgcaaat gccaatacgg tgccctacac ccttggagcg ctggaatcgg
     3121 cccaaatcgt tgccgaacgt ttcggtattt cggtggctga gttacgcaaa ctcaaccagt
     3181 ttcgtacgtt tgctcgaggt tttgataatg tccgccaggg tgatgaactg gatgtcccgg
     3241 cacaagttag tgaaaataat ttaaccccgc caccgggtaa tagcagtggc aaccttgagc
     3301 aacagatagc cagtacttca cagcaaatcg ggtctctgct cgccgaggat atgaacagcg
     3361 agcaagcggc aaatatggcg cgtggatggg cctcttctca ggcttcaggc gcaatgacag
     3421 actggttaag ccgcttcggt accgcaagaa tcacgctggg cgtggatgaa gattttagcc
     3481 tgaagaactc ccagttcgat tttctccatc cgtggtatga aacgcctgat aatctctttt
     3541 tcagtcagca tactctccat cgtactgacg agcgtacgca gattaacaac ggcttgggtt
     3601 ggcgtcattt cactcccaca tggatgtcgg gcatcaactt ctttttcgac cacgatctta
     3661 gccgttacca ctcccgcgcc ggcattggcg cggagtactg gcgcgactat ctaaaattaa
     3721 gcagtaacgg ctatttgcga ctgaccaact ggcgcagcgc acctgaactg gacaacgatt
     3781 atgaagcacg cccggccaat ggctgggatg tacgcgcaga aggctggcta cccgcctggc
     3841 cgcaccttgg cggtaaactg gtctatgaac agtattatgg cgatgaagtg gccctgttcg
     3901 ataaagatga tcggcaaagt aatcctcatg ccataaccgc tggacttaac tataccccct
     3961 tcccgctgat gaccttcagc gcggagcaac gccagggtaa acagggcgaa aatgacaccc
     4021 gttttgccgt cgattttacc tggcaacctg gaagcgcgat gcagaaacag cttgacccga
     4081 atgaagtcga tgcacggcgt agccttgcag gcagccgttt tgatctggtg gatcgcaaca
     4141 acaacatcgt tctggaatat cgcaaaaaag aactggttcg cctgaccctg acagaccccg
     4201 tgacagggaa gtcaggagaa gtgaaatcac tggtttcgtc gctacaaacc aaatatgccc
     4261 tgaaaggcta taacgtcgaa gccaccgctc tggaagctgc cggtggtaaa gtggttacaa
     4321 cgggtaaaga tattctggtt accctgccgg cgtaccggtt caccagtacg ccagaaaccg
     4381 ataacacctg gccgattgaa gtcaccgctg aagatgtcaa aggcaatttt tcgaatcgtg
     4441 aacagagcat ggtagtcgtt caggctccta cgctaagcca gaaagattcc tcggtatcgt
     4501 taagtagcca gacgttgagc gcggattccc attcaaccgc cacactgact tttattgcgc
     4561 atgatgcagc aggtaatcct gttatcgggc tggtgctctc gacgcgtcac gaaggtgttc
     4621 aggacatcac cctttctgac tggaaagata atggtgacgg aagctatacc cagatcctga
     4681 ccacaggagc gatgtctggc acgctgacgc tgatgccaca gctgaacggt gtggatgcgg
     4741 ctaaagcccc cgccgtggtg aatatcattt ctgtttcgtc atcccggact cactcgtcaa
     4801 ttaagattga taaggaccgt tatctctccg ggaatcctat cgaggtgacg gtagaactga
     4861 gagatgaaaa tgacaaacct gttaaggagc aaaaacagca actgaatacc gcagtcagca
     4921 tcgacaacgt gaaacctggt gtcactacag actggaaaga aaccgcagat ggcgtctata
     4981 aggcaaccta taccgcctat accaaaggca gtgggcttac tgcgaagctg ttaatgcaaa
     5041 actggaatga agatttgcat accgctggat ttatcatcga cgccaacccg cagtcagcga
     5101 aaattgcgac attatctgcc agcaataatg gtgtgctcgc caatgagaat gcagcaaaca
     5161 ccgtctcggt caatgtcgct gatgaaggaa gcaacccaat caatgatcat accgtcacgt
     5221 ttgcggtatt aagcggatcg gcaacttcct ttaacaatca aaacaccgca aaaacggatg
     5281 ttaatggtct ggcgactttt gatctgaaaa gtagtaagca ggaagacaac acggttgaag
     5341 tcacccttga aaatggcgtg aaacaaacgt taatcgtcag ttttgtcggc gactcgagta
     5401 ccgcgcaggt tgatctgcag aagtcgaaaa atgaagtggt cgctgacggc aatgacagtg
     5461 ccacaatgac cgcgacagtt cgggatgcaa aaggcaacct gctcaatgac gtcaaggtca
     5521 ccttcaatgt caattcagca gcagcgaaac tgagccaaac cgaagtgaat agccacgacg
     5581 ggatcgccac agctacgctg accagtttga aaaatggtga ttatacggtt acggcctctg
     5641 tgagctctgg ttctcaggct aatcaacagg tgatttttat cggtgatcaa agtactgctg
     5701 ccctgaccct cagtgtgcct tcaggtgata tcaccgtcac caacacagct ccgctacata
     5761 tgactgcaac cttgcaggat aaaaatggca atccactaaa agataaagaa atcaccttct
     5821 ctgtgccaaa cgacgtcgca agtcggttct cgattagcaa cagcggaaaa ggcatgacgg
     5881 atagcaacgg gactgcaatc gcctccctga ccggcacgtt agcgggcacg catatgatca
     5941 cggctcgtct ggctaacagc aatgtcagcg atacacagcc aatgacgttt gtggcggata
     6001 aagacagagc ggttgtcgtt ctgcaaacat cgaaagcgga aatcattggg aatggcgtgg
     6061 atgagacgac tctgacagca acagttaaag atccttttga taacgtggtt aaaaatcttt
     6121 cagtagtctt ccgcacctcc cccgcagaca cgcaactgag tctgaacgcg cgtaatacta
     6181 atgagaacgg tattgccgaa gttaccctta agggcacggt tttgggtgtt catacagccg
     6241 aagccatact gcttaacggc aacagagata cgaaaatcgt caatattgcg cccgatgcca
     6301 gcaacgcgca ggtcaccctg aacatccctg cacaacaggt ggtgacgaat aacagtgaca
     6361 gcgtgcagct gacggcgacg gtgaaagacc cgtcgaatca tccggtggcg ggaataacgg
     6421 tgaacttcac catgccacag gacgtggcgg caaactttac ccttgaaaat aacggtattg
     6481 ccatcactca ggccaatggc gaagcgcatg tcaccctcaa aggcaaaaaa gcgggcacgc
     6541 atactgtgac cgccacgctg ggtaacaata atgccagcga tgcgcaacca gtcaccttcg
     6601 tggcggataa ggacagcgcg gttgtcgttc tgcaaacatc gaaagcggaa atcattggga
     6661 atggcgtgga tgagacgact ctgacggcaa cagtgaaaga tccttttgat aacgcagtaa
     6721 aagatctaca ggtcaccttc agtaccaacc ccgcagatac tcaacttagt cagagcaaaa
     6781 gcaatactaa cgacagtggt gtggccgaag ttacctttaa gggcacggtt ttgggtgttc
     6841 atacagccga agccacactg cctaacggca acaacgatac gaagatagtc aatattgcgc
     6901 ccgatgccag caacgcgcag gttacgctga acatccctgc tcaacaggtg gtgacgaata
     6961 acagcgacag cgtgcagctg acggcgacgg tgaaagatcc gtcgaatcat ccggtggcgg
     7021 gaataacggt gaacttcacc atgccacagg acgtggcggc aaactttacc ctcgaaaata
     7081 acggtattgc catcacccag gccaatgggg aagcgcatgt cacgctcaaa ggtaaaaaag
     7141 cgggtacgca tacggttacc gcaacgctga gtaataacaa taccagtgat tcacagccgg
     7201 taacgtttgt ggcggacaaa acctcggctc tggttgttct tcagatatca aaaaatgaga
     7261 tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac ggtcaaagat cagttcgaca
     7321 atgaggtgaa caatcttccg gtaacattca gcacagcttc ttcaggcctc accctgaccc
     7381 caggggaaag taataccaat gagtctggca tcgcgcaggc cactctcgca ggcgttgcct
     7441 ttggtgagca gacggtcact gcatcactgg ctaataatgg tgccagcgac aacaaaactg
     7501 tgcattttat tggcgacaca gcggcggcaa aaattatcga gttgacgcct gtcccagaca
     7561 gcataatcgc aggtaccccg cagaacagct ccggcagcgt catcaccgcc acagtcgttg
     7621 ataataatgg ctttccggtg aaaggtgtga ctgtgaactt caccagcaac gcagcgacag
     7681 ccgaaatgac gaatggcggt caagccgtga cgaacgaaca gggtaaggct accgtcactt
     7741 ataccaatac ccgctcctcg atagaatcag gagcgagacc ggataccgtt gaggccagtc
     7801 tggaaaatgg tagctccacg cttagcacat caattaatgt caacgctgat gcgtctacgg
     7861 cacatctcac cttgctacag gcactttttg atacagtctc cgcaggcgac actaccaatc
     7921 tgtatattga ggtgaaggat aattacggca acggagtacc ccagcaggag gtaaccctca
     7981 gcgtttcacc aagtgaaggt gtgaccccca gtaataacgc tatatatacg accaatcacg
     8041 acggcaattt ttacgcaagc tttaccgcta caaaagccgg ggtataccaa gtgacggcaa
     8101 ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta tgtgccgaac gtagcgaatg
     8161 ctgaaatctc gctggcagcc tcgaaggatc cggtaattgc caacaataac gatctcacga
     8221 cactaacagc aacagtcgct gatacagagg gcaatgcgat agccaacagt gaggtaacat
     8281 ttactctgcc ggaagatgtg agggcgaact tcacgctggg cgatggcggt aaagtggtta
     8341 ctgatactga aggcaaagcg aaagtcacgc tgaaaggtac aaaagcaggc gctcatactg
     8401 ttacagcatc gatggctggc ggtaagagtg agcagttggt ggtgaacttt attgcggata
     8461 cactcactgc gcaggttaat cttaacgtta ccgaggacaa ttttatcgct aataacgtcg
     8521 ggatgaccag gctgcaggca acagtgactg atggaaacgg caacccgtta gccaatgagg
     8581 cggtgacatt cacgctaccg gcagatgtga gcgcaagctt tactctcgga caaggcggtt
     8641 ccgccattac tgacatcaac ggcaaggctg aagttacact gagcggtaca aaatccggca
     8701 cctaccccgt gacagttagc gtgaacaatt atggtgtcag tgatacgaaa caggtgactt
     8761 tgattgccga tgctggtacc gcaaaactag cctccttaac ctctgtatac tcattcgtcg
     8821 tcagcacgac cgagggcgcg accatgactg caagcgtcac tgacgctaac ggcaacccgg
     8881 tagaaggtat aaaagttaat ttccgcggaa cttccgtcac gctaagcagc accagcgttg
     8941 aaacggatga tcggggtttc gctgaaattc ttgtgacaag caccgaggtc ggactgaaaa
     9001 cagtttcagc ctctctggca gataaaccta ctgaagtcat ctcgcgatta ctgaatgcaa
     9061 aagcagatat taattctgca acgattacca gtctggagat acctgaaggt caggtcatgg
     9121 tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca gtttggcaat ccgattctta
     9181 atgaatctgt aacattcagt gcagaaccac cagagcacat gaccatcagc caaaatattg
     9241 tctctactga tacgcatggt atagccgagg tcactatgac gcccgaaaga aacggttcgt
     9301 atatggtgaa agcatccctg gcgaatggat cctcttatga gaaggatctg gtggtaatcg
     9361 atcaaaaact gacactctcg gcgtccagcc cgcttatcgg tgtcaattcc ccaacaggtg
     9421 caactctgac ggcaacgcta acttctgcaa atggcactcc agtggagggt caggtcatca
     9481 actttagcgt aacgccagaa ggtgcgacgt taagtggcgg aaaagtgaga accaactctt
     9541 caggtcaggc tccagtcgtt ctgaccagca ataaagtcgg tacatatacg gtgactgcat
     9601 cgttccataa cggcgtaaca atacagacac agacaatcgt gaaagtcact ggcaactcaa
     9661 gcaccgccca tgttgctagc tttatcgctg atccatcgac tatagccgcc accaacagtg
     9721 atttaagtac cttaaaggca acggttgagg atggcagtgg taacctgatc gaaggtctca
     9781 ctgtgtactt cgccttaaaa agcggctctg ccacattaac gtcattaaca gcggtgacag
     9841 atcaaaacgg aatcgcgaca acaagcgtga gaggagcgat aacggggagc gtcacggtaa
     9901 gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat aacgctggtg gcaggcccgg
     9961 cagacgcctc gcagtccgtc cttaagaaca atcggtcatc attgaaagga gactttaccg
    10021 atagtgctga gctacatctt gttctgcacg atatatcagg caatccgatc aaagtttctg
    10081 aagggctgga atttgtgcag tcaggtacca acgcgcccta tgtgcaagtt agtgcaattg
    10141 actacagtaa aaatttctca ggcgagtaca aagccactgt tacaggcggc ggagagggta
    10201 tcgcaacgct gatccctgta ttgaatggtg ttcatcaagc gggtctgagt accacaatac
    10261 aattcactcg cgcagaagac aaaataatga gcggtacagt gttagtcaat ggtgctaacc
    10321 taccgacaac tacattccct tcgcaggggt tcactggggc gtattatcag ttgaataatg
    10381 acaactttgc cccaggaaaa acggcggctg attatgagtt ttcaagctct gcctcctggg
    10441 ttgatgttga tgctaccggt aaagtgacat ttaaaaatgt cggcagcaaa tgggagagga
    10501 ttacggcgac gccaaaaaca ggcggcccta gctatatata cgaaatccga gtgaagagtt
    10561 ggtgggtgaa cgccggcgat gctttcatga tatacagcct tgctgaaaat ttttgcagta
    10621 gcaatggcta cacacttccc cttggagacc atttaaacca tagtcgttcc cgaggcatcg
    10681 ggtcactgta cagtgaatgg ggagatatgg ggcattacac gactgaagct ggttttcatt
    10741 caaatatgta ttggtcatcg agtcccgcaa actcaaacga acaatacgta gtttccctgg
    10801 caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc ttatgcgaca tgttataaaa
    10861 acctctgatt ttccgttata atttcttaaa gagtgctcca ttcggggcac tcttttttac
    10921 attccctcta tagagcttgc ccttctctca cagaaaatga ataaataaaa atgcgatacc
    10981 gcaagaatcg cgttcgatgt gactatgatc gtatccggtg agtacagctt ttatccaaag
    11041 gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccacc gttcataaca
    11101 gatcggcatt actatgcata agtactgtct catattctcg ccaaaacact ttttaatgcc
    11161 tttgcgaatt catccagact tatattgtca attttttgcc caacgacgac tgactccgaa
    11221 acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac aacgggggta
    11281 ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcgat ataagcatct
    11341 tttatgattg ctgctgaacg tttaatcgag ggtgataagg ataaacggta gacattatta
    11401 taacaatcca ctaataccct ggctttatct tccccttttg gtccatgaac gatcactatt
    11461 ggtatatctg tttcactttg aattttttct attagatttt ctgcaatcga tagtgaaaat
    11521 gtacgatcct gcgagctacc ttctaaatta agcgcaatgt aagatcctaa cgatcgcatt
    11581 tcctcgcgca cctcatcgag tacatcctca cttagtggca attcatatat tggcctgact
    11641 gctggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacggg agcccggagt
    11701 ttctgatcca tccgggacgc gttcttacac acaggggaat agcacttcat ggttaaacca
    11761 acaacctgaa aattcgtttt tgctttcaac tgactgataa ataacatcgt tttcaggttc
    11821 tttttacgca tcgcctctat acaaagatcc ggcgtaccgt attgctgtgt tatgttcttc
    11881 gctaaatctt ttatttcttt taatgttgcg tgatcctgca tagtcattgt gactaatgtt
    11941 aatttagtct gttcaagttt gagcgcatta aagacttcta aattaattgt cgacgtaaca
    12001 attaatagat gcttaatttt atgcaattca agcgcccgaa taacaggaaa gatggccata
    12061 gcatcgccaa tctgatcggg aatatggatg acaacaaagt ctgttttttc aatattgaaa
    12121 ttataagctt tataatcgta gtaactaaat gcaatacgtc tcaacaatga tactaaaaac
    12181 ataactaacc tcgcctccct actggttata atgcaatgca gtctatcaga atcatcaggg
    12241 tgcaattttg tgcatatgcg gacttttatg tttcatatct cgaacctgtg gggcctctgc
    12301 ttaatcctta aacaacacca gcaactcctg cgctttcatc ttccagcgaa tttttcatgt
    12361 tgccgttaat cagccataaa atcatttgca gatgcgctct gtcgaggtag tctcataagg
    12421 ttcgtttata gatcgacgca atgtgagtta ccttttccat actaattata aaaagacagt
    12481 acaaacagga tcattatgga ctccacgctc atctccactc gtcccgatga aaggacggtt
    12541 tcgttaagtc gcgcccgacg agctgcgtta ggcagtttcg ctggtgccgt cgtcgactgg
    12601 tatgattttt tactctatgg cattaccgcc gcactggtgt ttaatcgcga gtttttcccg
    12661 caagtaagcc cggctatggg aacgctcgcc gcatttgcca cctttggcgt cggattcctt
    12721 ttccgtccgc tcggcggtgt cattttcggt cactttggcg accgactggg acgtaagcgc
    12781 atgttaatgc tgaccgtctg gatgatgggc atcgcgacag ccttgattgg tattcttcct
    12841 tcattctcga ccattgggtg gtgggcacct attttactgg tgacgctgcg tgccattcag
    12901 ggatttgccg tcggcggcga atggggaggc gcggcgttgc tttccgttga aagtgcaccg
    12961 aaaaataaaa aagcctttta cagtagcggt gtacaagttg gctacggtgt aggtttactg
    13021 ctttcaaccg gactggtttc attgatcagt atgatgacga ctgacgaaca gtttttaagc
    13081 tggggctggc gcattccttt cctgtttagc atcgtactgg tactgggagc attgtgggtg
    13141 cgcaatggca tggaagagtc cgcggaattt gaacaacagc aatataatca agcggccgcg
    13201 aaaaaacgca tcccggttat cgaagcgctg ttacgacatc ccggtgcttt cctgaagatt
    13261 attgcgctac gactgtgcga gttgctgaca atgtacatcg ttaccgcctt tgcacttaat
    13321 tattcaactc agaatatggg gttaccgcgc gaacttttcc ttaatattgg tttgctggta
    13381 ggtggattaa gctgcctgac aattccctgt tttgcctggc ttgccgatcg ttttggtcgc
    13441 cgcagggttt atatcacagg cgcgttgatc ggaacgttga gcgcatttcc tttctttatg
    13501 gcgcttgaag cacaatctat tttctggata gttttcttct ccataatgct ggcaaacatt
    13561 gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt tggtgccagt
    13621 tatcgctata gtggtgctgg agtcggttat caggttgcca gtgtggttgg cggtggattt
    13681 acacctttta ttgccgctgc gctcatcact tactttgccg ggaactggca tagcgtcgcc
    13741 atttatttgc tggctggatg tctgatttcc gcaatgaccg ctttgttgat gaaagacaat
    13801 caacgcgctt gatagtctgg cgaagatcat ccgatcttcg ccttacactt ttgtttcaca
    13861 ttcctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca cacatgaata
    13921 ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg ctgtatgagc
    13981 aatctgtagt cgcattacgc aacgccattg gcaactatat tacaagtggc gaattacctg
    14041 atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc tctgaccgta acctgggacg
    14101 gtagcacaac aaatcccccc aaaacgcgcg catttggtcg ttttactcac gcaggcagct
    14161 acaccaccac gattactcgc cctactctct ttcgttcgta tcttaatgaa caacttacgt
    14221 tgctgtatca ggattatggc gcgcatatct cggtgcaacc ctcacagcat gaaatccctt
    14281 atccttatgt catcgatggc tctgaattaa cacttgatcg ctcaatgagc gctgggttaa
    14341 ctcgctattt cccgacaaca gaactggcgc aaattggcga tgaaactgca gacggcattt
    14401 atcatccaac tgaattctcc ccgctatcgc attttgatgc gcgccgcgtc gatttttccc
    14461 tcgcgcggtt gcgccattat accggtacgc cagttgaaca ttttcagccg ttcgtcttgt
    14521 ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc cagatcctcg
    14581 atcctgatag tccctacatt gccctttctt gtgctggcgg gaactggatc accgccgaaa
    14641 ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag atgccagcat
    14701 ggcatttaat taccgccgat ggtcagggta ttactctggt gaatattggc gtgggaccgt
    14761 caaatgctaa aaccatctgc gatcatctgg cagtgctacg cccggatgtc tggttgatga
    14821 ttggtcactg tggcggatta cgtgaaagtc aggccattgg cgattatgta cttgcacacg
    14881 cttatttacg cgatgaccac gttcttgatg cggttctgcc acccgatatt cctattccga
    14941 gcattgctga agtgcaacgt gcgctttatg acgccaccaa gctggtgagc ggcaggcccg
    15001 gtgaggaagt caaacagcgg ctacgtactg gtactgtggt aaccacagat gacaggaact
    15061 gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc gtagcaattg
    15121 atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca tacgggacac
    15181 tactgtgtgt ttcagataaa ccgttgcatg gcgagattaa acttcccggt caggctaacc
    15241 gtttttatga aggcgctatt tccgaacacc tacaaattgg cattcgggcg atcgatttgc
    15301 tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg aacctttaat gagccgccgt
    15361 tccgataata agttctgaac cttcttatca aaaatagatg tgaaggatta agtaagacat
    15421 ctggcaaata gcccgcaata aacgcgggct gt
//
LOCUS       DBFIFS010000104        15276 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.104, whole genome shotgun sequence.
ACCESSION   DBFIFS010000104 DBFIFS010000000
VERSION     DBFIFS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.104"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            90..608
                     /locus_tag="N7A07_002811"
     CDS             90..608
                     /locus_tag="N7A07_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU2477441.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            624..1163
                     /locus_tag="N7A07_002812"
     CDS             624..1163
                     /locus_tag="N7A07_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU2477442.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1256..2833)
                     /gene="guaA"
                     /locus_tag="N7A07_002813"
     CDS             complement(1256..2833)
                     /gene="guaA"
                     /locus_tag="N7A07_002813"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU2477443.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2902..4368)
                     /gene="guaB"
                     /locus_tag="N7A07_002814"
     CDS             complement(2902..4368)
                     /gene="guaB"
                     /locus_tag="N7A07_002814"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU2477444.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4530..5900
                     /gene="xseA"
                     /locus_tag="N7A07_002815"
     CDS             4530..5900
                     /gene="xseA"
                     /locus_tag="N7A07_002815"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU2477445.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5897..6112)
                     /locus_tag="N7A07_002816"
     CDS             complement(5897..6112)
                     /locus_tag="N7A07_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU2477446.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6181..7653)
                     /gene="der"
                     /locus_tag="N7A07_002817"
     CDS             complement(6181..7653)
                     /gene="der"
                     /locus_tag="N7A07_002817"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU2477447.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7771..8949)
                     /gene="bamB"
                     /locus_tag="N7A07_002818"
     CDS             complement(7771..8949)
                     /gene="bamB"
                     /locus_tag="N7A07_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU2477448.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8960..9580)
                     /gene="yfgM"
                     /locus_tag="N7A07_002819"
     CDS             complement(8960..9580)
                     /gene="yfgM"
                     /locus_tag="N7A07_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU2477449.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9598..10872)
                     /gene="hisS"
                     /locus_tag="N7A07_002820"
     CDS             complement(9598..10872)
                     /gene="hisS"
                     /locus_tag="N7A07_002820"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU2477450.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10983..12101)
                     /gene="ispG"
                     /locus_tag="N7A07_002821"
                     /gene_synonym="gcpE"
     CDS             complement(10983..12101)
                     /gene="ispG"
                     /locus_tag="N7A07_002821"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU2477451.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12128..13141)
                     /gene="rodZ"
                     /locus_tag="N7A07_002822"
     CDS             complement(12128..13141)
                     /gene="rodZ"
                     /locus_tag="N7A07_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU2477452.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13426..14580)
                     /locus_tag="N7A07_002823"
     CDS             complement(13426..14580)
                     /locus_tag="N7A07_002823"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU2477453.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14730..15161)
                     /gene="ndk"
                     /locus_tag="N7A07_002824"
     CDS             complement(14730..15161)
                     /gene="ndk"
                     /locus_tag="N7A07_002824"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU2477454.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
BASE COUNT         3717 a         4187 c         3769 g         3603 t
ORIGIN      
        1 taatccatcc ctttatacgc aatacattta ctttcctctt ttgatgatct taaatgtctt
       61 atttttcgta atgtgtataa caaggaatag tgatgaaatt taaaaaatgt cttctgcctg
      121 tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgac gatcatgccg
      181 ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc gttaatatta
      241 tttccattct tcccgcaaaa gttgccgtag acaactccca aaataaacgg aacgcacaag
      301 ccttcggcgc gcttattggc gcagtcgctg gcggtgttat cggccacaac gtcgggtctg
      361 gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg agctgtaggc gcggcagcgg
      421 gttctatggt gaatgataaa accttagtgg aaggtgtttc tttaacgtat aaggaaggca
      481 ccaaagtgta tacctctact caggtgggta aagagtgcca gtttacgaca ggtttagccg
      541 ttgttattac cacgacgtat aacgaaacgc gtattcagcc aaataccaaa tgtcctgaaa
      601 agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc ttagcctcct
      661 taagctatcc ggctatcgcc tcatcattgc aggatcaact ctctgctgtc gcagaagcgg
      721 aacagcaagg taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg gagcgcaaca
      781 gggaaatcca gcaagagaag caacgtcgcg caaatgccca ggctgccgct aacaaaagag
      841 cggcaacggc agcggcaaat aagaaagctc gtcaggataa actggacgcc gaagccactg
      901 cggacaaaaa acgcgatcaa agttatgaag atgagctacg cagcttagag attcagaaac
      961 aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg agaaaacgaa tttatcgatc
     1021 aggagctgaa gcacaaagcc gcgcaaactg atgtcgtgca atctgaagct gacgccaaca
     1081 gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca gaagagaata
     1141 aatcggacag ctggtttaat taatcgatgt tagtaacttc aagcctataa ttcttgaaga
     1201 taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg tcaaatcatt
     1261 cccactcaat ggtagctggt ggcttgccgc tgatgtcata caccacgcgg gaaataccgt
     1321 tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa atcgtacggc agatgcgccc
     1381 agtgtgcggt cataaagtcg atggtttcga cagcacgcag agagacaacc cagtcatact
     1441 tacgaccatc gcccattacg ccaacggaac gtactggcag gaagacggtg aacgcctggc
     1501 tgactttgtc gtacaggtcc gctttacgca gttcttcaat gaagatggcg tcagcacggc
     1561 gcagcaggtc acagtactct ttcttcactt cacccagaac acgaacgcca aggcctggtc
     1621 ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc cagctccaga ccaatcttac
     1681 gcacttcgtc tttgaacagc tctttcagcg gttcaaccag gcccatcttc atctctttcg
     1741 gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt gcagacgccg
     1801 cagattcgat aacgtcaggg tagatggtgc cctgcgccag ccacttcacg tcttccagtt
     1861 tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc gatgatttta cgttttgctt
     1921 ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc ttctgccggt acgtgaacaa
     1981 tgttaagacc aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg ttgagacgca
     2041 gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc gatagcgcgg tgcagcagca
     2101 ttgcggttac ggaggaatcc acaccaccag agaggccgag gatgacttta tcgtcgccta
     2161 cctgctcgcg gatgcgagct acagcatcgt cgataatttt cgctggcgtc cacagggctt
     2221 cacactggca gatatcacgc acaaaacgct ccagcatgcg cataccctgg cgggtgtgag
     2281 tcacttccgg gtggaactgt acgccataga agcgtttttc ttcgttagcc ataatggcaa
     2341 acgggcagct ttcggtgctg gctacggtga cgaagtcgga cgggatagcg gtaactttat
     2401 cgccgtggct catccagaca tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga
     2461 taccgcgaac cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt
     2521 cgttagaggc ttcaacgtga ccgcccaact gcattgccat ggtctgcatg ccatagcaaa
     2581 cgccgaatac cggtacgcct gcttcaaaga catactgcgg cgcacgcggg ctgttttctt
     2641 cagtagtact ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt
     2701 gtgcttctgt cacatcccat gcccacagtt cgcagtaaac acccagctca cgcacgcggc
     2761 gcgcaaccag ttgagtgtac tgagaaccga agtccagaat gaggatgcga tgcttatgaa
     2821 tgttttccgt cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga
     2881 catgaagtcg ggcgaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat
     2941 ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc
     3001 tttagtacgc agttcgtcga tagtaccaca gccggtcaaa cccatacagg agcgcaggcc
     3061 gcccatctgc tggtgaatga tctctttcag gcgaccttta taagctacgc gaccttcgat
     3121 accttccggc accagtttgt cggcagcgtt atcgctctgg aaataacggt cagaggaacc
     3181 tttggacatc gcgcccaggg aacccatacc acggtaagat ttgtaagaac ggccctggta
     3241 gagttcgatt tcacccggag attcttcagt acccgccagc atggaaccta ccatcaccgc
     3301 gcttgcgcca gcggcgatag ctttggcgat gtcgccggag aagcgaatac caccatctgc
     3361 gataaccgga ataccggtgc cttccagggc ttcaactgcg tcagcaacgg cagtgatctg
     3421 cggaacacct acgccagtaa cgatacgagt cgtacagata gaaccagggc cgataccgac
     3481 tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca cctgcagctg ttgccacgtt
     3541 gccgccgatg atttgcagat ccggatattt agcacgggtt tcacggatac gctgcaaaac
     3601 gccttctgag tgaccgtggg aggagtcgat cagcagaacg tcaacgccag cggcaaccag
     3661 cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa cacgcagacg
     3721 gccttgctcg tctttacagg cgttcggttt acgttccgct ttctggaaat ctttcacggt
     3781 gatcatgccg atcaggtgga attcgtcatc aaccaccagc gctttttcaa cgcgtttttc
     3841 gtgcattttt gccagcacca cttcacgggc ttcaccttca cgcacggtga ccagacgctc
     3901 tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg
     3961 accggtgata atccccacca gttcgttttc ttcggtaacg accggatagc ccgcaaaacc
     4021 gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg
     4081 atcagtcacc acaccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc
     4141 aatggacatg tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag
     4201 gcgcgcttcc gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt
     4261 tttcgtcagc tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac
     4321 gaggagaacg tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc
     4381 gaccagagtg gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta
     4441 cccctttttg caaaaaatgc ttgctatccc cgaagggcgg gttactatcg actgaataac
     4501 ctgctgattt agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta
     4561 ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt
     4621 ggatcagcgg cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac
     4681 tcaaagacga caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga
     4741 ccttccgccc acagcatggg caacaagttt tagttcgcgc caatattacg ctctacgagc
     4801 cgcgcggcga ctaccagata atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc
     4861 aacagaagta cgaacagctc aaagcgaagt tgcaggctga aggattgttc gatctgcaat
     4921 acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg
     4981 ctgcgctaca tgatattttg catgtgttaa aacgtcgcga tccttctctg ccggtgatca
     5041 tctaccccac cgccgttcag ggcgatgacg cgccggggca aattgttcgc gccattgaac
     5101 tggcgaatca gtgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg
     5161 aagatttatg gagttttaac gacgaacgcg tagcgcgggc gatttttgcc agccgcattc
     5221 cggtcgtcag cgccgtcggg catgagacgg atgtgaccat tgccgatttt gttgccgatc
     5281 tgcgtgcacc aacaccgtcg gctgccgccg aagtggtgag ccgtaatcag caagagttac
     5341 tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc gatggattat tatctcgcca
     5401 accgcacacg tcgctttacg caaattcatc accgattaca gcaacagcat ccgcagctcc
     5461 ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa acggatgagc tttgcgctgg
     5521 aaaatcaact taagcgtgcc gggcaacagc agcagcgatt aacacagcgg ctgaatcagc
     5581 aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt
     5641 tagcagaaac cctgcgcgca cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa
     5701 cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg tggatacagc gttactactg
     5761 ctactgacgg caaggtactg aaaaaagtga agcaggttaa agcgggtgaa atgctaacca
     5821 cacgtctgga agacggctgg gtagaaagtg aagtaaaaaa catccagccg gtaaaaaaat
     5881 cgcgtaaaaa agtgcattaa gccagcacga attcaacgcg ttttttcgat atcaagccat
     5941 gcccgtgctg acagaagtaa tctaccgcac cgcaggcttt taacacctgc aacggttgat
     6001 ggcagtccgg gcaaagagct ttcatttcaa taactttccc gcaggatggg caatacgcat
     6061 ggccgttatc ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattc
     6121 ctccggtagc cacccctcta cattcataga gggaatggca gataaaatac ttacggataa
     6181 ttatttattt ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt
     6241 cagggtgtta cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg
     6301 cgatcccatt acgtccagcg atttgcggaa gtagttcatc aagtaacgct tgtaggaatc
     6361 aggcaggtct ttcacctgat taccgtgaat caccacaatc ggcggattat aaccaccggc
     6421 gtgtgcatat ttcagcttca cacgacgacc gcgtaccagc ggcggttggt gatcttcaac
     6481 agccatcgtc atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata
     6541 cgcttcacgt actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa
     6601 gtgcacacga gcaaaatcga taaagcccag acggaagtcc agcgtttctt tcacctgctc
     6661 tttcacttcc tgactcaggc catcccactt attcaccaca atgacaagtg agcgcccact
     6721 attgagaata aagcccagca gcgagagatc ctgatcggaa ataccttcgc gcgcatcaat
     6781 cactaacatc accacgttgg cgtcttcaat ggcctgcaac gttttgatta cggagaattt
     6841 ctctacagca tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata
     6901 ctcacgtcca tcgcgttcca ttggaatgta gatactgtca cgcgtcgtac caggcatgtc
     6961 ataaacaaca acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt
     7021 cggacgaccc acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc
     7081 ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag tattcagcgt cttcgtcgac
     7141 ttcctcttgc ggtgcgagat cttccatcca cggcagtagc acatgctcca gcagacttaa
     7201 tacgccacga ccgtgagacg cggcgatcgg gtagatttca cctaaaccaa gcgagtagaa
     7261 atcaaccact gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg
     7321 tttttcacgg gagcgcagat gtttggcaat cgcttcatct gccggcatca ggcccgcgcg
     7381 cgcatccacc ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc
     7441 catgcgggtt tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa tacagataaa
     7501 ctcacggcct tcaatttccg cacgaccgta cttacggtca cgagtcagac ccgggaaatc
     7561 cgcaaccagc gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt
     7621 agggcgcccg acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat
     7681 cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag gaaaacggcc cctgtccagg
     7741 agccgttttc aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc
     7801 ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga aaccggaact
     7861 atcaactttt tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcaaataacc
     7921 ttcactgtca ccgaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg
     7981 cagcagatcg ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg
     8041 gtcattttga tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc
     8101 cagttcgcgt ttccacataa tctgaccact gcgcagatca agcgccgtca ggttaccatt
     8161 ataggccagc gcgaaaacaa cgccgttaac aacggcggga gtcgtgtcaa catcgctcag
     8221 acggtcaatt tcggtagaac cggtcgcttg ggaaatacgc tgctgccaaa tcatctggcc
     8281 ctgttccatc agcactgcgc tgacgcgacc attatcgccc cccacgacgg ccgcaccaaa
     8341 agccgttgcc ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt
     8401 ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat
     8461 taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg
     8521 ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat
     8581 gtagacatgt ccaccagaca cggtcacacc gccagaaagt aatgcaggct ctttagagaa
     8641 ccagccatct ttctcggcca ggctgacaga ccagatttct ttgccatcat ccgcattcag
     8701 cgcttttact aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg
     8761 aagattggaa tagaagttgc caatgccgct accaacggaa gtgctccacg ccgtggtcgg
     8821 cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt
     8881 aaacagcgaa cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg
     8941 caattgcatc gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc
     9001 tcagtgccgg agtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct
     9061 tatcaccttt gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt
     9121 cacctttgat ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc tgtacctgaa
     9181 cgcgagcaag acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc
     9241 cctgttgtaa ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa
     9301 gttccaaaga agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg
     9361 ccgggatgct atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag
     9421 aagcggagcg tgcagaatca acctgatggc tgttccagta gcgccagcca atcagtgcgc
     9481 caacgcccaa aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa
     9541 tcgcttctac ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta
     9601 acccagtaac gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg tttgctcacc
     9661 agagcgcaaa tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac
     9721 agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc
     9781 gtggttggtc atcaatttca cgcccggtaa ttcatcacgc agacgctcag ctaatgccat
     9841 agccgcagat tgtgtatcag cacctgaagc caccaggtat atatcgacaa caggatcggc
     9901 tttaaattcc ggattaacgg cctgtactaa caatacaaga cgttcgaggc ccatcgcaaa
     9961 accgacagcc ggtgttgcac ggccgcccag ttgttccaca agaccgtcat aacgaccgcc
    10021 tgcacacacg gtgccctggg agccgagact gttagtcacc cactcgaaaa cggtacggtt
    10081 gtagtaatcc agaccacgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc
    10141 aagcagtttg cacagaccgg caaaatgctc acgagattcc tcgtccagat aatcacctaa
    10201 tgccggagcg tcgttgagaa gcgcctgcac ttccggattt tttgaatcca gcacgcgcag
    10261 cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag
    10321 gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc agcgaaccga tagaattcag
    10381 ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac cagcgggcgg tgagcataat
    10441 cagttcagcg tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa
    10501 ctgacgataa cgccctttct gcggacgctc gtgacggaac atcggcccga tataccacag
    10561 acgctgttcc tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt
    10621 cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt
    10681 ttcaaccacg tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat
    10741 cggcaagcgg atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat
    10801 gcgctgccag atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat
    10861 gttttttgcc acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca
    10921 atgtttcggg agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt
    10981 tattattttt caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc
    11041 gcacgaatgc gtgcctccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc
    11101 acgccatctt catagagacc gcttttcttg ttgccgccgg tgacgccgag tgtagaaacc
    11161 agcgcctcac ccgggccatt caccacgcag ccgataatcg aaacgtccat cggagtgatg
    11221 atatcttcca ggcgttgctc cagcgcgtta actgtaccga taacatcaaa ttcctgacgc
    11281 gaacaggtcg gacaggcgat gaagttgata cctcgcgaac ggatacgaag cgatttcaaa
    11341 atatcgaaac cgactttgat ctcttcgacc ggatcggccg ccagtgatac gcgcagcgtg
    11401 tcgccgatgc cttcagacag cagcagacct aaaccaatgg cggattttac tgccccgctg
    11461 cgcgcgccac ccgcttcggt gatccccaga tgcagcggct gatcgatttg ttttgccagc
    11521 aaacgataag actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga
    11581 tcgaagttca ggcgatcgag atgatcaaca tggcgcatgg cggattccag caacgcctgc
    11641 ggcgtcggtt cgccatactt ttcttgcaga tctttttcca gcgatccggc gttaacgcca
    11701 atacggatcg gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca
    11761 ttaccgatat tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc
    11821 gcaatgcgat agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg
    11881 agtttgaacg cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca
    11941 acgcgttcca gcgccttgat ttgattgacc gttgcttcga catctgtcgt acgcgtattg
    12001 gtcatggact gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata
    12061 cgtgttgatt ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac
    12121 ccgtctgtta ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag
    12181 ttctgataaa acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg
    12241 gcgcaccaat tttcagtttg tacggtgcct ggccggttaa gttcaaatta ccgtctttac
    12301 gctgcatacc gctaaacaat tttttaccgg tagcatcagt gacctccagc cagcaatcgg
    12361 cagtaaagtt catcaccagc gcattcggat cagccaccgg cgtggtcacg ccagcctgat
    12421 cggttggcaa cggcgcagca ccatctggcg ttgttgctgc cgttggtgcc ggggtcgcgg
    12481 cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg
    12541 gtgctggcgc agttacgaca ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg
    12601 gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga acactctgcc
    12661 cctgctcgct attactgctc agttccgccg aagattgatc ggccatagtg gtgatctctt
    12721 cctgctgagc tttgtggtct tgccaccacc aggcaccgct caggccgata accacaaaca
    12781 acaccagcca ggtgaaggtc atcagccagc cgtcgcgttt tttgcggcgt ttaccgaggg
    12841 aaaaactctg catcggcgca acttttgcag cccgtagtgg agcctgcttt tccagccctg
    12901 gcagcagttc ttcttctgga atatgtacca gacgcgcata agagcggata tatccgcgca
    12961 ggaatgttga agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa
    13021 ccttcaggca aagtcgctcg gcaacggcct gctgactaag tcctagttgt tcgcgagcat
    13081 tacgcaggcg agcgccggta gtaagtgctt cattttggtc gtgcgtggct tcagtattca
    13141 ttcgctgcag gtacgtttaa atgagaattg agatgccggt gaaccatcat gctcacccac
    13201 accgcgaaac atccgttaag ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg
    13261 tgacaaagcc cacattcact aaggctaact tactgttgca tcgttacata ctgccttaaa
    13321 gtcagcaaaa acgcaccgtt aatattgacc agacaattgc aacttaatac ttcattcata
    13381 aaacattacg ccacggtaca taaagtaacc gtggcgtaat ggctatcaga ccgctttaat
    13441 gtcgatggct tcaccctgca tccgtttacg cagggtacgt ttcgtacggt cgataacatc
    13501 gcccgccaac tgaccacagg cagcatcgat atcatcacca cgcgttttac gcacaatagt
    13561 ggtgaaaccg tagctcatca gcacttttga gaaacggtcg atacggctgt tcgagctgcg
    13621 tccatacggc gcagccggga acgggttcca cgggatcagg ttgatcttac acggcgtatc
    13681 tttcagcagt tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat
    13741 cacgtattca atagtgactc ggccctgatt ggcgttggat ttctccagat aacggcgcac
    13801 cgcagcaagg aacgtttcga tattgtactt tttgttgatc ggcacaattt cgtcacgaat
    13861 ttcgtcgttc ggcgcgtgca gggaaattgc cagcgcaaca tcgatcatat cgcccagttt
    13921 atccagcgct ggaactacac cggaagtgga aagcgtcacg cgacgtttag acagaccaaa
    13981 accgaaatca tcaagcatga tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg
    14041 ctcgcccatg cccatcatca ctacgttagt gattgggcgc tgaccggtga cttttgctgc
    14101 gccgacgatt ttcgccgcac gccacacctg gccaataatt tccgataccc gcaggttgcg
    14161 gttaaagccc tgctgggcgg tggaacagaa tttacactcc agcgcacacc ccacctgcga
    14221 agagacgcag agcgtagcgc ggtcgtcttc cgggatgtac accgtttcga cacgctggtc
    14281 acccacggca atcgcccatt taatggtgcc gtcagatgaa cgctgttctt caaccacttc
    14341 cggtgcgcgg atctccgcca cctctttcag tttgccgcgc aacactttgt tgatgtcggt
    14401 catctcatca aaattgtcgc agcaatagtg atacatccac ttcatcacct gatcggcgcg
    14461 gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc tgctgacgat tgagatccag
    14521 caggttgatt tttccatctt tggtggtgac gttttcaggt gtgactaatt gttcagacat
    14581 atgctattcc ggcctcgtta ttacacgtta tggcccctgg agggttgaaa aaagaaacgc
    14641 cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac aaattctggc gcacggatgc
    14701 cacgtttgca cgcggcattt acgaaattat taacgggtgc gcgggcacac ttcgccttcg
    14761 ccgaagaaat aagcgatttc gcgagcggca gattcgacgg aatcagaacc gtgggtaccg
    14821 ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac cggccagtgc gtttgccgga
    14881 ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt tttcaccttc cagcacggaa
    14941 accacgatcg ggccagaggt catgaattca accagaccat caaagaacgg ttttccatcg
    15001 tgttcagcat aaaagccacg tgcctgttca acggtcaggt gcagcatttt ggtgccgaca
    15061 attttgaacc ctgcagcttc aaagcgcgca aagatattac caatgacgtt ttttgctacc
    15121 gcgttcggtt tgataatgga aaaagtacgt tcaatagcca tttttacctc tgtaaattgt
    15181 tctgttgttg tctgtaccag cgtacgagat ggcgcggatt ataatgagca acagagccgt
    15241 tgactattga tgaaggtaac atttttttaa aataaa
//
LOCUS       DBFIFS010000105        15227 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.105, whole genome shotgun sequence.
ACCESSION   DBFIFS010000105 DBFIFS010000000
VERSION     DBFIFS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.105"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..2209
                     /locus_tag="N7A07_002825"
     CDS             <1..2209
                     /locus_tag="N7A07_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2477455.1"
                     /translation="AASFVPVETESDPMNVAFDKLAPEVQNAVMVKFDTCENITVDMV
                     ISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQA
                     EMRIWKKRREGERKETGKYTSVVDLARARVHRQHTENSAEKIPPVTAVIRREYKQTWK
                     TLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRKQPDALRYDR
                     QTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVPETDAV
                     ETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALAAGATEI
                     TKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKKIMQAANISQPDANQLLA
                     ASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQKAEQNSPNTQQNEPETKQ
                     PEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEG
                     AEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITSTSRTCDHLMIDLETMGKNP
                     DAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTD
                     EIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRD
                     VRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSVIWQKLIPNQADF"
     gene            2274..2522
                     /locus_tag="N7A07_002826"
     CDS             2274..2522
                     /locus_tag="N7A07_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2477456.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            2500..3630
                     /locus_tag="N7A07_002827"
     CDS             2500..3630
                     /locus_tag="N7A07_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2477457.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(3676..4314)
                     /gene="ompW"
                     /locus_tag="N7A07_002828"
     CDS             complement(3676..4314)
                     /gene="ompW"
                     /locus_tag="N7A07_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU2477458.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            4554..4643
                     /locus_tag="N7A07_002829"
                     /pseudo
     CDS             4554..4643
                     /locus_tag="N7A07_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4671..5414
                     /gene="yciC"
                     /locus_tag="N7A07_002830"
     CDS             4671..5414
                     /gene="yciC"
                     /locus_tag="N7A07_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU2477459.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            5444..5983
                     /gene="yciB"
                     /locus_tag="N7A07_002831"
     CDS             5444..5983
                     /gene="yciB"
                     /locus_tag="N7A07_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU2477460.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6088..6486
                     /gene="yciA"
                     /locus_tag="N7A07_002832"
     CDS             6088..6486
                     /gene="yciA"
                     /locus_tag="N7A07_002832"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU2477461.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(6526..7245)
                     /gene="tonB"
                     /locus_tag="N7A07_002833"
     CDS             complement(6526..7245)
                     /gene="tonB"
                     /locus_tag="N7A07_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU2477462.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            7469..7765
                     /gene="yciI"
                     /locus_tag="N7A07_002834"
     CDS             7469..7765
                     /gene="yciI"
                     /locus_tag="N7A07_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU2477463.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            8066..9319
                     /gene="kch"
                     /locus_tag="N7A07_002835"
     CDS             8066..9319
                     /gene="kch"
                     /locus_tag="N7A07_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU2477464.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9374..9547)
                     /gene="yciY"
                     /locus_tag="N7A07_002836"
     CDS             complement(9374..9547)
                     /gene="yciY"
                     /locus_tag="N7A07_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU2477465.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            9690..11150
                     /gene="cls"
                     /locus_tag="N7A07_002837"
     CDS             9690..11150
                     /gene="cls"
                     /locus_tag="N7A07_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU2477466.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            11185..11514
                     /gene="yciU"
                     /locus_tag="N7A07_002838"
     CDS             11185..11514
                     /gene="yciU"
                     /locus_tag="N7A07_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU2477467.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(11567..12571)
                     /gene="oppF"
                     /locus_tag="N7A07_002839"
     CDS             complement(11567..12571)
                     /gene="oppF"
                     /locus_tag="N7A07_002839"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU2477468.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(12568..13581)
                     /gene="oppD"
                     /locus_tag="N7A07_002840"
     CDS             complement(12568..13581)
                     /gene="oppD"
                     /locus_tag="N7A07_002840"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU2477469.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(13593..14501)
                     /gene="oppC"
                     /locus_tag="N7A07_002841"
     CDS             complement(13593..14501)
                     /gene="oppC"
                     /locus_tag="N7A07_002841"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU2477470.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(14516..>15227)
                     /gene="oppB"
                     /locus_tag="N7A07_002842"
     CDS             complement(14516..>15227)
                     /gene="oppB"
                     /locus_tag="N7A07_002842"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU2477471.1"
                     /translation="AHGDFGPSFKYKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVS
                     AGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHWLPGGGWNGGALK
                     FMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALL
                     PVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTIL
                     FNAIVDVLYAVIDPKIRY"
BASE COUNT         4025 a         3739 c         3664 g         3799 t
ORIGIN      
        1 ggctgcttcc tttgtcccgg ttgagactga atccgatccg atgaacgtcg cttttgacaa
       61 gctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac
      121 cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa gacatggcaa cattcgacgg
      181 acatatcgtt gaagcgttga tgaaaatgcc agaagttaac gccatgtatc cggagcttaa
      241 gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat ggcccgaaat
      301 tcaggcagaa atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa
      361 atacacgtct gttgttgatc tcgcccgcgc cagagtccac cgacagcaca ctgaaaactc
      421 agcagaaaaa atcccccctg tcactgcagt cattcgtcgc gaatataagc agacatggaa
      481 aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg ccggaaacat
      541 tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacgacc gcgaagactg
      601 gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg accgccagac
      661 tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg tggcactgaa
      721 caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag gaacaaatca
      781 gagcgcaact gacactctct cgtcgccagt accagaaact gatgcagtgg aaacggcaat
      841 tccggacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag agcgtgaggg
      901 gccgttctac ttcctcttca ccgacaagga tggcgaaaaa tacggtcgcg caaacaaact
      961 ttctggtctg gataaggcgc tggctgccgg ggctactgaa atcacaaaag aagaatattt
     1021 tgcccgaaaa aatggcacat acacaggctt accgcaaaat gcaaataccg cacaaaattc
     1081 tgaacaacca gaaccggtaa aagttaccgc tgacgaagta aagaaaatta tgcaggcagc
     1141 caatatcagc cagcctgacg ccaatcagtt gctcgccgca tcacgtggtg aatttgttgc
     1201 agggattagc gacccgaatg atccgaaatg ggtgaagggg attgaaaccc gcgattctgt
     1261 gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa gcggaacaaa acagcccaaa
     1321 tacgcaacaa aacgagccag aaacgaaaca acctgagcca gtagcgcaac aggaaccgga
     1381 aaaagtctgc accgcctgcg gtcagaccgg cggcggcaac tgccctgatt gtggcgcggt
     1441 gatgggcgac gcaacatacc aggaaacatt cgatgaagag tatcaggttg aagttcagga
     1501 agatgatccg gaggaaatgg aaggcgctga acatccacac aaggagaaca ctgacggcaa
     1561 tcagcatcac gatagcgata atgaaactgg cgagacggca gatcactcaa ttaaggtgaa
     1621 cggtcatcaa gaaatcacat ccaccagcag gacgtgtgac catctaatga tcgaccttga
     1681 aaccatggga aaaaatcctg atgccccgat catctcaata ggtgcaatat ttttcgatcc
     1741 gcaaaccgga gatatgggac cggaatttag taagactatc gatctggaaa ctgctggcgg
     1801 ggtcattgat cgggacacca ttaaatggtg gcttaagcaa tcacgcgaag cgcaatctgc
     1861 cattatgacc gatgaaatcc cgttagatga tgcactatta caattgcggg aatttatcga
     1921 cgaaaactcc ggtgaatttt ttgttcaggt ctggggaaat ggagccaact tcgacaacac
     1981 gattttgcgc cgttcatacg aacggcaggg gatcccctgc ccgtggcgtt actacaacga
     2041 tcgcgatgta cgcacaatcg ttgagctggg gaaagccata gacttcgatg ccagaacggc
     2101 tattccattc gaaggtgagc gccataatgc acttgatgac gcccgttacc aggcaaaata
     2161 cgtttcagtt atctggcaaa aactgatccc gaatcaggct gatttttaat gttcaacccc
     2221 ggtcgttgcc caccagctat agtggcggcg accatgatta gcgaacgacg ctcatggcaa
     2281 gacttattct gctcactgag tgggcaaaag aggaattcag tgaaccggtc ccaactccga
     2341 gtacgttaag taaatacgct aaagccggaa tgatatttcc tctccccaaa aaagttggaa
     2401 gacgctggcg agtggatccg caagctcgct ttgtcggaat ggtaaacaag ccggaggtga
     2461 tcgccacaga tcaccctgct ttgaagagga tactggaaga tggcgcgccc gcgaaaatat
     2521 aaaaccgatg ttccgggatt atctccgtat tttgacaaaa gaaataacaa agtttactgg
     2581 cgttacaggc atcccataac aggcaaaaat cacggtctcg gcagtattga ccagaaactg
     2641 gcagaaacta ttgcagcaga agcgaacagc cgtcttgccc ggcagcaaat ggaacaaatg
     2701 ctcagtctgc aggagaaaat tattagtgat accggcggtt catcaaccgt taccattttt
     2761 ctgaataatt acagaaaaat tcaacaggaa agatatgaaa acggcgagat caaactcaac
     2821 acgctgaaac agaaagcggc ccctctcagg gtatttgatg aacgttttgg caccagaccg
     2881 ttagatgcca taaccgtaaa agatgtggta tcagtactgg aagagtacaa ggccagagga
     2941 cataacagaa tgggacaaat tttcaggaaa gtactgatcg atgttttccg ggaagctcag
     3001 caaacgggcg atgtcccgcc aggctttaac cctgcagaat cggcaaaaaa accgcaggtg
     3061 cggatatcaa gacagcgact gacttttgat gagtggatga tgatttataa cgcagcggaa
     3121 aaggatggtt actttttaca gcgcggtatg ctgctggcac tgatgacagg ccagcgcctt
     3181 tcagatattt gcaaaatgca attttcggat atccgggatg gttatcttca tgtcgaacag
     3241 caaaaaacag gaacccggat tgccatccct ctggctctgc gttgcgataa attaaatctc
     3301 accctggatg atgtggtgtc atcctgccgc gattgcgttc ttagtccgtg gctattgcac
     3361 caccatcacg cgaaagggac agctaagcgc ggcgggatgg ttaagccagc aacattaacc
     3421 gttgcattta aaaaagcccg ggattctgtg gattacaact ggcgtgctaa tggcacccct
     3481 ccctctttcc atgagcagag atctttatca gagcgattgt tcagagagca gggggttgat
     3541 accaaaattt tgctaggcca ttcgaatcaa aaaatgaccg atatttacaa cgacgcacgc
     3601 ggtaaggaat ggaaaaaact ggtcatttga tgaccagttt tgcagagggg ttttgcagag
     3661 gttttgcaga gaaaattaaa aacgatatcc tgcggagaac ataaacaccc acggatccag
     3721 tcgtaccgag tcttttacgg tagtaacacc agatttgtac ttagccgtgg tatcgatatc
     3781 catgtaccac actgacatgt taaccaacca gtcacggtta atcagataat caacccccac
     3841 ctgcccggca gctccccagg aatctttcag actgagatcg gaaagccccg cctctttgcc
     3901 atgatcgtta aatccattat caaagaaggt ggtgtagtta atacctgccc caacataagg
     3961 acggaatttg ctgctggcat caccaaaata ccactgcgcc atcagtgttg gcggcagatg
     4021 atgaacggtt gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga acggcgtcgc
     4081 tgccagtaat tccacaccaa tgttgtcggt cgccatataa gtaaacgtca ggcccagttg
     4141 agtgttattg gtcacgctga atccacccag acttcctaac gtaccaccag caccttctgt
     4201 tggacgtacg gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat gcgcaaaggc
     4261 actgccagag agaagagttg ttactgccaa agccgccact gttaactttt tcatatccgc
     4321 tccgtcgtta tggttataaa aacgtgacga atatacctac attggagtaa caaatgatcc
     4381 tacatagatc acattacata tgataattca ccatttattg atctggatta atttagaagt
     4441 tgcattgaga atcctggtta acgtgattta aatcaatttt taaatatttt cgctacgcaa
     4501 atattaactc gttcgccact tgccgcggtt tgcccacgcc tgaaggccag caattacctc
     4561 cgctttacaa agatatgctt ttccatacaa gacctgatgc tgccacccga ggcttaacca
     4621 gggtacaatt gcccgctaat taacacctgc ataaaactca aggagagtgc atgtctatca
     4681 cggcgcagtc cgtataccgt gacaccggaa atttcttccg taatcaattt atgaccattc
     4741 tgttggtatc gttgctatgt gcgtttatca cagtggtgtt agggcatgtt ttctcaccca
     4801 gtgatgcaca gcttgcgcag ctcaatgacg gcgtgcccgt tagcggcagt agtgggttgt
     4861 tcgacctggt tcagaatatg tcaccggaac agcaacaaat tttgctgcag gcttcagcgg
     4921 cgtccacttt ttcaggatta atcggtaacg ccattctcgc cggaggcgta atattaatta
     4981 tccagctggt gtctgcgggt cagagagtca gtgcgctgcg agctattggt gccagtgcgc
     5041 cgatattgcc aaagttattt attctgattt ttctgactac ccttttagta cagattggca
     5101 tcatgctggt ggtcgttccg ggaattatca tggccatatt actggccctg gcaccggtga
     5161 tgctggtaca ggacaaaatg ggcgtttttg cctcgatccg tagcagtatg cggctgactt
     5221 gggcgaatat gcgtctggtg gcacccgcag tactgagctg gttgctggca aaaacactgt
     5281 tgctgctttt tgcctcttct tttgccgcat taaccccgga aattggtgcc gtactggcga
     5341 acaccttgag caacctgatt tcagccatat tgctcatcta tctgttccgc ctgtatatgt
     5401 tgattcgcca ataaccttaa gcatttgatt acggaatcgt aaaatgaagc agtttcttga
     5461 ttttttaccg ctggttgtct ttttcgcgtt ttacaaaatt tatgacatct atgcggctac
     5521 tgcggcgctg atcgtcgcca cggcgattgt gcttatctat agctgggttc gctttcgtaa
     5581 ggttgagaag atggccctga tcacttttgt tctggtggtc gtcttcggtg gcttgacgct
     5641 gttcttccac aatgatgagt ttattaaatg gaaggttaca gtcatttatg ctctgtttgc
     5701 gggagccctg ttagtcagcc aatgggtgat gaaaaagccg ctaattcagc ggatgctggg
     5761 taaagaactc acgctgccgc aaccggtatg gtcgaagctg aatctggcct gggctgtttt
     5821 ctttatcctt tgcggtctgg caaacatcta catcgcattc tggctgccgc aaaatatttg
     5881 ggtcaacttt aaagtctttg gcctgaccgc ccttacctta atcttcacat tgttaagcgg
     5941 tatctatatc taccgccaca tgccgcagga agataaatcc taaccagact gccagtcctt
     6001 tggggctggc gctgtatctc ctctgttctc ctcaggaaaa tcatagtagc atcgcgcctg
     6061 tgattttcct tttaagtcgg ttttaccatg tctacaacac ataacgtccc tcagggcgat
     6121 cttgttttac gtactttagc catgcccgcc gataccaatg ccaatggtga catctttggt
     6181 ggttggttaa tgtcacaaat ggatattggc ggcgctattc tggcaaaaga aattgcccac
     6241 ggtcgcgtag tgactgtgcg ggttgaagga atgactttct tacggccggt tgcggtcggc
     6301 gatgtggtgt gctgctatgc acgctgtgtc cagaaaggga cgacatcggt cagcattaat
     6361 attgaagtgt gggtgaaaaa agtagcgtct gaaccaatcg ggcaacgcta taaagcgaca
     6421 gacgcattat ttaagtatgt cgcggttgat cctgaaggaa aacctcgcgc cttacctgtt
     6481 gagtaatagt caaaagcctc cggtcggagg cttttgactt tctgcttact gaatttcggt
     6541 ggtgccgtta attttaaaca ggatattcac cacaatccca ctgcctggct tacccggctc
     6601 ataacgccat ctgcgcatcg catttttcac ctcacgctca aacatgttcg caggcttggc
     6661 tgagaggatt tgtacgttat ccacgcgacc atccggcgtg acgtcaaatt taactttaac
     6721 ctgcccttca atgcgcaatg cctgtgctcg tgccggatac tgcggctgat tacggcttaa
     6781 tgcgcgtggt cctgaagcca cactggtaac cggcttgctg gttgcagccg ttgctgtact
     6841 tgatgtcggg cgtgccggtg ccgtattttc aaacggtgat gccggacgcg actctacggg
     6901 tttgacatcg cgtttttgct gctcctgtac ctttttcacc ggcttcggtt ttggcttagg
     6961 tttcggcttc ggcttttcaa tgaccaccgg tgcttctttt ggcggttcgg ggatcggctc
     7021 aggttccggt tctggctcta ccaccggctc cggtggcggc tgaacggctt gtggcggttc
     7081 gagatcagca ggcgcaacca tcgtgacaga aatcggctgc gcaggcgcag gtagttcaat
     7141 aacctgatgt accgaggtat agagcagacc cgccacaaca gcaccatgaa tgcagaccga
     7201 aagtaacgtc ggccagggga agcggcgagg taaatcaagg gtcattgaag tcataatcat
     7261 ttcagtagaa aaaccaggtc tcgattttaa atgcaaatag caatcatatt caataaggca
     7321 agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact ttatgcatgg
     7381 tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata acgtacttta
     7441 caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc aagacaaagc
     7501 tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt tacagttact
     7561 gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca gtaacgatcc
     7621 aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc tggaagcagc
     7681 tcaggcctgg gccgatgctg acccgtacgt agcggcagga gtctatgaac acgtttcagt
     7741 gaaaccattt aagaaagtgt tctgagtaga gatccaatat ttaagggcac ggtttttgca
     7801 accgtgttct tgtttggcct ttccttatag gtcggtcatt gcattcacca ccacaagcag
     7861 ataataactt tatctgtatt tctttatatg atgtgagcat gttcaggaaa aatgcccttt
     7921 cctgacgcag agttgctcac tcaaaacctt tgattactgc ggataaagtc atctactatt
     7981 gtttaacaca ttcacgtgtc gcatgtatgt tcagaaaatg tgcacgttaa gataattaaa
     8041 caaaattgtg gttacagagg tatcagtgag tcactgggct acattcaaac aaacagcaac
     8101 aaatttatgg gtgaccctac ggcacgacat tctcgcgctg gccgtctttt taaatgggtt
     8161 gcttattttt aaaacaatct atggtatgtc ggtcaatttg cttgatattt tccatatcaa
     8221 agcattttca gagttggatc tctccttgct ggcaaacgcc ccactattta tgctcggcgt
     8281 ctttcttgtc ctgaactcca ttggcttact gttccgggca aagctcgcat gggcaatcag
     8341 tatcattttg ttgttgatag cgctaattta caccctgcat ttttatccct ggctgaaatt
     8401 tagtattgga ttttgcattt ttacgctggt gtttttactg atactgcgca aagacttctc
     8461 ccacagtagc gccgcagccg ggacaatttt tgcatttatt agtttcacga cattactgtt
     8521 ttactcaacc tacggtgcgc tttatttaag cgaaggtttt aatccgcgaa tagaaagttt
     8581 gatgaccgcg ttctattttt cgatagaaac catgtcaacc gtcggctatg gcgatattgt
     8641 ccctgtttct gaatcagcac gattgttcac tatttcggtc attatttccg gcattaccgt
     8701 ttttgccaca tctatgacct caatttttgg tccgcttatc cgcgggggat tcaacaaact
     8761 tgtaaaagga aacaatcata caatgcatcg taaagatcat tttattgttt gcggacattc
     8821 gattctcgcc attaatacga tcctgcaact gaatcaacgc ggacaaaacg taacggttat
     8881 cagcaacttg cctgaagatg atatcaagca acttgagcaa cgcttaggcg ataacgctga
     8941 tgttatcccc ggtgacagta atgacagttc tgtattaaag aaagcgggaa tcgatcgttg
     9001 ccgggccatt ctggcgctga gtgataacga tgcagataac gcgtttgttg tactctcggc
     9061 aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc agtgatagca aaaacctgaa
     9121 taagattaag atggtacatc cggatatcat tctctcaccg caactgtttg gcagcgaaat
     9181 tctggcgcga gttttaaatg gtgaagagat taataatgat atgctcgttt caatgttgtt
     9241 gaactcaggt catggtattt tcagcgataa cgatgaacaa gaaacgaaag ctgacagtaa
     9301 agaatcggcg caaaaataga gtttaaagac ggaagagtaa acttattggg caccgcaatg
     9361 gtgcccagta catttagata ttgtagatcg caaacaataa cgagttacgc tgtttgttta
     9421 gaatgcactt cctgatgctg tggatacgca tatttcggcg cgactgcccc tcaagccaac
     9481 gcgatttacg tcggctagcc tgacgctgca tgcgccagcg ccccacttcc gttctactcc
     9541 gcttcatgtt aactactcta tgcaataaca gaatggtcat tatacccccg cccccgacgc
     9601 tgaccagtgc ttttcccgcg tttttatttg ccagatgaat ccatatgcgt aaactcataa
     9661 caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt ttatacgttg gtgagttggt
     9721 tggccattct gggatactgg ttgctcattg caggcgtaac tttacgcatt ctaatgaaac
     9781 gacgcgcagt tccctccgcg atggcctggc tgttgattat ttacattctg ccgttagtcg
     9841 gaattattgc ctatcttgcc gttggcgagc tccatttagg caaacgccgc gctgagcgcg
     9901 ccagagcgat gtggccttcc accgcaaaat ggcttaacga ccttaaagcc tgtaagcata
     9961 tctttgccga agaaaatagc agtgtcgctg cgccattatt caagctttgc gagcgtcgtc
    10021 aggggatcgc tggggtcaaa gggaatcagc tacaactgat gaccgagtca gatgatgtga
    10081 tgcaggcgtt aatccgcgac atccagctcg cgcgccataa tattgagatg gtgttttata
    10141 tctggcagcc cggcggcatg gcggaccagg tggctgaatc attaatggcg gctgcgcgac
    10201 gcggcattca ttgccgattg atgctcgact ccgccgggag tgtagctttt ttccacagcc
    10261 cgtggcccga gctaatgcgt aatgccggta ttgaagtggt cgaagcctta aaggtcaatc
    10321 tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca tcgcaagatg atcatgatcg
    10381 ataattacat cgcgtacacc ggcagcatga atatggtcga ccctcgctac ttcaaacaag
    10441 atgcgggcgt agggcaatgg attgatctga tggcgcgtat ggaaggcccc atcgccaccg
    10501 cgatggggat tatttattcc tgcgactggg agattgaaac agggaaacgt attctaccgc
    10561 caccacctga tgtcaatatt atgccgtttg agcaggccag cggtcatacc attcacacaa
    10621 ttgcttctgg ccccggcttt ccggaagatc tcattcacca ggcattattg actgcggctt
    10681 attcggcgcg tgaatatttg atcatgacca cgccctactt tgtgccaagc gatgatttac
    10741 ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt cagtattatc cttccgcgaa
    10801 aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt ctttacggaa ctgcttgctg
    10861 ctggggttaa agtttatcag tttgaaggcg ggttactgca taccaagagc gtgctggtcg
    10921 atggcgaact aagtctggtt ggcaccgtta accttgatat gcgtagtctg tggctgaatt
    10981 tcgagattac cctggcaatc gacgataaag gttttggtgc agaccttgcc gccgttcagg
    11041 acgattatat ttcgcgttca cgtctgctcg atgcccgttt atggctaaaa cgtccattat
    11101 ggcaacgtgt cgccgagcga ctgttttact tcttcagccc gttgctgtaa aacgtcgcca
    11161 acagaggtta aacaggtagt gactatggat atggatctaa acaatcgcct gactgaagat
    11221 gaaacgcttg agcaggccta cgatattttt ctcgaactgg ctgccgacaa tctcgatcca
    11281 gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg gcggcgcgga gttattcgat
    11341 ccggcggaag actggcagga acatgttgat ttcgatttga acccagactt ttttgccgaa
    11401 gttgtgattg gtctggcgga cagtgaagat ggtgaaatca acgatgtttt cgcccgcatt
    11461 ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca tctggcggga ataaataatc
    11521 actttcggcg tgaaaaaggg ctgacagctg tcagcccttt ttgtgtttaa agcggatcga
    11581 ctttcaggca agaaacggcg tgtctgaaac tcccctccag cacaggacgt gttttggcac
    11641 actccggacc ggcaatcggg caacgggtac ggaaaacaca accggaaggc gggttgatcg
    11701 gcgacggtaa ttccccttcc agtaactgga tggttttgtt cttctccaga tccggatcag
    11761 gtatggggac tgccgacatc aatgccctgg tgtaaggatg tagtggattg tggtagacct
    11821 catcataggt ccccagttct accgcatggc cgagatacat caccaacaca cgatcggaaa
    11881 tgtgttttac cacggccagg tcatgagcga taaaaattaa tgacaatccc atctcacgtt
    11941 gcaattgctg gagcaggttg accacctgtg cctgaattga cacgtccagc gccgacaccg
    12001 gctcatcaca gataatcagc ttcggttcaa gaataagagc acgcgcaatc ccgatacgct
    12061 ggcactgccc accagagaac tcatgcggat agcggttaat caggttaggc aataacccga
    12121 ctttcagcat catcgccttc acgcgctcgc gaacttcctg gcgtgacatt ttcggatgat
    12181 aagtacgcag tggttcagcg atgatctcgc cgatggtcat acgcgggttt agcgatgcca
    12241 acggatcctg gaaaatcatc tgaatatcac tgcgaacggc acgccattca tcgggtttca
    12301 tgcccagcaa ctctttacct aaccaggcaa catgaccgtc ggtcgcttta accaggccaa
    12361 tgatggcgcg agcaaaggtg gacttaccgc atcccgattc ccctaccaca cctaatgttt
    12421 ccccttcata caggcgaaga gttacaccat ctacggcttt gagcgttttc ggcggttgcc
    12481 agaaccactg tttgccatct ttgatttcaa agtgcacttt cagatcggcg atttcgagga
    12541 ggacttttct tccttcggtt acagcattca taacagttcc tccaccggtt taaagcaagc
    12601 acgcagacgg ccaggcgtaa actcttccag cggcggtgcg ctactacaaa tttccatcgc
    12661 atgcggacaa cgtggctgga atgggcaacc tttcggtaat cgcagcaggt ttggcggatt
    12721 acccggaata gtcaacattg tttcaccttc cgcatcgaga cgaggcaccg cgttgagcaa
    12781 accgatagaa taaggatgaa cgggttgata aaagacatcg cgcgcgttgc catattccat
    12841 cgtgcgcccg gcgtacatta ccagcacttt gtcgcagatc cccgccacca cgccaagatc
    12901 gtgggtgatc ataatgatgg cggtattaaa ttcccgcttc agttcattca atagcgtcat
    12961 gatctgcgcc tgtacggtga cgtccagcgc agtagtgggt tcatccgcaa tcagcagctt
    13021 aggtcgacat agcaatgcca tcgcaatcat aactcgctga cgcatgccgc cagaaaattc
    13081 gtgcgggtac attttcatgc gtttacgtgc ttccggcatt tttaccgcat cgagcatccg
    13141 caccgactct tcaaacgcct cagctttgct catgttctta tgcagcatca gcacttccat
    13201 caactgctca ccgacgcgca tatagggatt caacgaagtc attgggtcct gaaaaatcat
    13261 tgagatttgt tcagcgcgca gtttattgag ttcacgctct ggcaaattga ggatttcacg
    13321 cccattgaag gttgccgatc cgccaatgcg tccgttggca gccaacaggc ccatcaacgc
    13381 aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca atgcccagcg tttctccggc
    13441 acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg ccgtccgggg tactaaaggt
    13501 gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt tgttgtgcga gcggcacagt
    13561 tgcagtttca attacgctca tggctgcact ccttaacgat ctttcgggtc gagggcatca
    13621 cgcaagccat cgccgataaa gttgaaacaa aacagcgtca ccacgaggaa tcccgctggg
    13681 aacaacagta accatggaga gacttccatt gagttcgcgc catcactcag caatgccccc
    13741 cagctgctta acggctcttg cgtacccaac cccaggaagc taaggaaaga ttcaaagagg
    13801 atcatgctgg gcaccagtag cgatgcgtag accaccacca caccgagtac gttcggcaca
    13861 atgtggcgaa taacaatgcc cgacgtcgat acaccgccaa cttgtgccgc ctcaataaac
    13921 tctttgcgct tcagactcag ggtttgccca cgcacaatac gagccatatc cagccaggaa
    13981 accatgccta tcgccacgaa aatcagcagg atgttttgac cgaaaaaggt caccagcaaa
    14041 atgacgaaga acatgaatgg gaaggagttg aggatttcca gcaaacgcat cattaccgaa
    14101 tccactttac cgcccagata accggaaagc gaaccataaa gtgtccccac gaccactgcc
    14161 accagtgccg cagcaacacc gaccatgagt gagatacgtc cgccaatcgc aacgcgcaca
    14221 agcaggtcgc gaccggatga gtcagtacca aagtagtgac cggactccat atccggggcg
    14281 ctggacatca tcgcccagtc agtatcgtca taggcaaact gcgaaagcat cggtgccagg
    14341 attacaaata acgcgatcag caccagcact atcagacttg caaccgccgc acggttatgc
    14401 ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt cgacctccag cttttcactg
    14461 aaattttcca gcgtctcgct gtttttctta cttaacatca tcgcgagctc cagtatcagt
    14521 aacggatttt cgggtcgata accgcatata gcacatcgac aatggcatta aacaaaatgg
    14581 ttaacgcacc aaccaggatg gtcaggctta acactaagga atagtcacgg ttcaatgcgc
    14641 cattaacgaa cagttgccca atccccggca aaccataaat ggtttcgata accatagaac
    14701 cggtaataat gccgacaaat gcggggccca tataggagag cacgggtaac agagcaggtt
    14761 ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc tttcgcccgg gcagtacgaa
    14821 taaagttgga gtgtaatact tcaatcatag agccacgggt aatacgcgca atactggcga
    14881 tataggcgag tgacaacgcc accatcggca atatcatgaa tttaagcgcc ccaccattcc
    14941 agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat catgactaat aatggcgcaa
    15001 ccacaaaact ggggataaca accccggtca ttgccagccc catcacggta tagtcccatt
    15061 tggtgttttg ttttaatgcg gcaataacgc cagcactaac acccagtatt accgcaagga
    15121 aaaatgctgc ggctcccagt ttggcagaaa cgggaaaact ggatgcaacc aggtcattga
    15181 ccgaataatc tttatattta aacgatggac cgaaatcgcc atgcgcc
//
LOCUS       DBFIFS010000106        15114 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.106, whole genome shotgun sequence.
ACCESSION   DBFIFS010000106 DBFIFS010000000
VERSION     DBFIFS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.106"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(45..659)
                     /locus_tag="N7A07_002843"
     CDS             complement(45..659)
                     /locus_tag="N7A07_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU2477472.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            928..1128
                     /gene="glgS"
                     /locus_tag="N7A07_002844"
     CDS             928..1128
                     /gene="glgS"
                     /locus_tag="N7A07_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU2477473.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            complement(1163..1462)
                     /gene="ubiK"
                     /locus_tag="N7A07_002845"
     CDS             complement(1163..1462)
                     /gene="ubiK"
                     /locus_tag="N7A07_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU2477474.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     regulatory      1584..1732
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1836..2489
                     /gene="ribB"
                     /locus_tag="N7A07_002846"
     CDS             1836..2489
                     /gene="ribB"
                     /locus_tag="N7A07_002846"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU2477475.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            2751..2921
                     /gene="yqiD"
                     /locus_tag="N7A07_002847"
     CDS             2751..2921
                     /gene="yqiD"
                     /locus_tag="N7A07_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU2477476.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(2979..3752)
                     /gene="zupT"
                     /locus_tag="N7A07_002848"
     CDS             complement(2979..3752)
                     /gene="zupT"
                     /locus_tag="N7A07_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU2477477.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            3895..4683
                     /gene="ygiD"
                     /locus_tag="N7A07_002849"
     CDS             3895..4683
                     /gene="ygiD"
                     /locus_tag="N7A07_002849"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU2477478.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(4721..5881)
                     /locus_tag="N7A07_002850"
     CDS             complement(4721..5881)
                     /locus_tag="N7A07_002850"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU2477479.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(5887..6558)
                     /locus_tag="N7A07_002851"
     CDS             complement(5887..6558)
                     /locus_tag="N7A07_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU2477480.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(6706..8187)
                     /gene="tolC"
                     /locus_tag="N7A07_002852"
     CDS             complement(6706..8187)
                     /gene="tolC"
                     /locus_tag="N7A07_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU2477481.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8392..9021
                     /gene="nudF"
                     /locus_tag="N7A07_002853"
     CDS             8392..9021
                     /gene="nudF"
                     /locus_tag="N7A07_002853"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU2477482.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            9022..9444
                     /locus_tag="N7A07_002854"
     CDS             9022..9444
                     /locus_tag="N7A07_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU2477483.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            9469..10296
                     /gene="cpdA"
                     /locus_tag="N7A07_002855"
     CDS             9469..10296
                     /gene="cpdA"
                     /locus_tag="N7A07_002855"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU2477484.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            10296..10877
                     /gene="yqiA"
                     /locus_tag="N7A07_002856"
     CDS             10296..10877
                     /gene="yqiA"
                     /locus_tag="N7A07_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU2477485.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            10906..12798
                     /gene="parE"
                     /locus_tag="N7A07_002857"
     CDS             10906..12798
                     /gene="parE"
                     /locus_tag="N7A07_002857"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU2477486.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(12862..15003)
                     /locus_tag="N7A07_002858"
     CDS             complement(12862..15003)
                     /locus_tag="N7A07_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HGU2477487.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT         3660 a         3629 c         3950 g         3875 t
ORIGIN      
        1 cattattaaa tttccttttg attaataaat tacaatattt ggggctaggg attgttttcc
       61 gccaaatatc gcgttgcaga aagtcggcaa ataattaaca ctttgtctcc tttagtgaaa
      121 atttttcctt cttcaggctc cagtagtaaa taatgtattt ggccaaaatg atcggtgagt
      181 tttccttcgc agggattacc tgaagtggct tgatgaccgg taattaacgc catgctacca
      241 atatattctt cttctgtaat agctgagcta tgatcgcgtg gaatccaggg cgcaactatt
      301 ttaccggtat aatgcaccgc aataatcgta aatagtaaac tgacaggaac cacaaacagg
      361 ttcgagagtg gtgattgcca gaccatgatg caggtatgct gcaacaggat gccaataaga
      421 ccaaaaaaac cagccagtaa gcaaagaacg acgagagctg gcaatcttcc gatattgaga
      481 taatgaagtg cctggctaat atgacctgtg gttatggaat cataatggtc aagatgtgca
      541 tcaagagcac cggagagcat gtgaccgcag ataagcgcaa aaatttctaa aaggccgatc
      601 aacagtacaa aggaaatagc gaaaagataa ggggtgttat agtcggcgaa taaaatcata
      661 tgtcactcca atgtccattt agtgacgcaa tatttacttt gtgatagttt ccttgcttac
      721 atttgtcttt gttaataata acatatgtta gagaggttct ggaagagaaa gtcttttaat
      781 atggtttttt atttttcact aaaaagtgtg atcggggaca atatatttac gcacgttatg
      841 tttaaaggca ctacactgat tggggaatac tgaaatcaga aaaagcatat ggccttaaaa
      901 ggtatgcgat aaccggagga tgtgcttatg gatcatagtc ttaattcttt aaataatttc
      961 gatttcctag cgcgtagttt tgccagaatg cacgcagaag gtcgcccggt cgatattctg
     1021 gccgttactg gtaacatgga tgaagaacat agaacctggt tttgcgcacg ttatgcctgg
     1081 tattgccaac agatgatgca aaccagagaa cttgaattag aacactgata ttacgggccg
     1141 tctggcccgt ttttgtgttg ctttattctg ctttatcaat aggcgggata gccggaggag
     1201 attgaagatc tgctaccgaa ttattgcgcg cttccagttc actgctgcgc tgctccagta
     1261 acgccagttt ttcacgagtc cgcaacagga cttgcgtctg aacgtcgaat tcttcgcggc
     1321 ttaccagatc gagacgcgtc agctgcgctt gtagggtttg gcggattttt ttctccacat
     1381 cttccccgaa ctccctgatt cctttaggca ttgattcgtg aacctggcga gcgatttgct
     1441 caattttttt cgggtcaatc attgtagttt ccctgtactg ataggtgttg agtgccattg
     1501 tagtgcgata agggtaagtc ataaaccaga attatgtgaa gctatgcgtt gctgccgcta
     1561 atcattagcg ttatagtgaa tccgcttatt ctcagggcgg ggcgaaattc cccaccggcg
     1621 gtaaatcaac tcagttgaaa gcccgcgagc gctttgggtg cgaactcaaa ggacagcaga
     1681 tccggtgtaa ttccggggcc gacggttaga gtccggatgg gagagagtaa cgattctgtc
     1741 gggcatggac ccgctcacgt tattttggct atatgccgcc actcctaaga ctgccctgat
     1801 tctggtaacc ataattttag tgaggttttt ttaccatgaa tcagacgcta ctttcctctt
     1861 ttggtacgcc tttcgaacgt gttgaaaatg cactggctgc gctgcgtgaa ggacgcggtg
     1921 taatggtgct tgatgatgaa gaccgtgaaa acgaaggtga tatgatcttc ccggcagaaa
     1981 ccatgactgt tgagcagatg gcgctgacca ttcgccacgg tagtggtatt gtttgcctgt
     2041 gcattactga agatcgccgt aaacaactcg atctgccaat gatggtagaa aataacacca
     2101 gcgcctatgg caccggtttt accgtgacca ttgaagcggc tgaaggtgtg actaccggtg
     2161 tttctgccgc tgaccgtatt acgaccgttc gcgcagcgat tgccgatggc gcaaaacctt
     2221 ctgatctgaa tcgcccgggc cacgttttcc cactccgcgc tcaggctggt ggtgtactga
     2281 cgcgtggcgg tcatactgaa gcaactattg atctaatgac gctggcaggc ttcaaaccgg
     2341 ctggtgtgct gtgtgagctg actaatgacg atggcacgat ggcgcgtgca ccagagtgca
     2401 ttgagtttgc caataaacac aacatggcgc tcgtgactat tgaagacctg gtggcatacc
     2461 gtcaggcaca tgagcgtaaa gccagctgaa aaccgctgct taatttactg ccttaatcaa
     2521 gaaaccgaag ttgtagcagg cttcggtttt tatttttccc tactatgcaa gatgttaatt
     2581 ccgcttctct ttctatgaga aaatttcatt aatatcaggc attctttttc attatgatca
     2641 aaatcatata attgttccgt tattatgcga ccatatgaat atatccgaaa aaggatatgt
     2701 gaaatttatg cgctaaatgt ttagtattgt taaatattaa gggtaaaagg atgtttatcg
     2761 cctggtactg gattgtattg attgttctgg ttgtggtggg ttatttcctg catttgaaac
     2821 gttattgtcg ggcatttcgc caggacagag acgcactgct tgaagcgcgg aacaaatact
     2881 taaacagtac gagagaagag acagccgaaa aggtagaata aggctttagt gaaactagca
     2941 gaagttgtcc ctgcgctgga caacttctga tgttgctttt aaccaattcc cgccgtttgt
     3001 agcagcacca aactgaatcc catcactgac attccacata gtacgccata gctggggtta
     3061 ttattagggt caatctcttt ggcgagcggc attaattcat caaccgagag cgccaccata
     3121 attcctgcga ccgccgccat gattgctgcc atgaccaccg gggaaatcat gctaccgagg
     3181 attaaccacg ccagcacacc accaagaatt tctgccagtc cagaaatccc cgcccacaga
     3241 attgccgtgc gtttagaacc cgttgctgca taaaccgggc ctgcaaccgc cagaccttca
     3301 ggaatattgt gcaacgcgac ggccagtgcg atgccaaatc ccagctccag gttgctgctc
     3361 gccgtgacaa aggtggcaat cccttccggg aagttatgca gactgatgcc gagagtgagc
     3421 agaatggctg tgcgcttgat cgattttggc aacggctgca ccgatttttg cattaaatcc
     3481 tgcggatgcg catgcggcaa catgcggtcc aggccaaaat agccaagcag accgaagata
     3541 aacattccat aacccaacac aggcgacatt ccttcagctg ctagtgcggc aggaagcatt
     3601 tccattaatg agatgagcaa catgatcccc gccgcaaatc ctagcgaaaa cgccagtaag
     3661 cggttcgagg gtttttgccc gagaacgccg agaaacgcgc caataaacgt ggctgccccc
     3721 gccagtatgg tcagaatgag aggtactgac atcgacaact ccttataaat cttcaccttc
     3781 aggcagatcg tcaaaataac tgatgatcat catcattgtt atccgggttc ttactccgcc
     3841 gaacaagcgt atcgtgagga tatcaaaatg acacctttag ttaaggatat catcatgtct
     3901 tcaacacgta tgccagcatt gtttttaggt cacggtagtc cgatgaacgt gctggaagat
     3961 aatttgtata cccgcagctg gcagacgttg gggatgacat tgccacgccc gcaagcgatt
     4021 gtggtagttt cggctcactg gtttacccgt ggaacaggag tgaccgcgat ggagacgccg
     4081 cccacgattc atgactttgg tggcttcccg caggcgctgt acgatacgca ttatcctgct
     4141 ccgggttcgc ctgcgctggc acagcgtctg gttgagctgt tagcgccgat cccggtgacg
     4201 ctggataaag aagcctgggg ctttgaccac ggttcctggg gcgtgctgat taagatgtat
     4261 cctgacgcgg atatcccgat ggtgcagttg agtatcgaca gtagcaaacc tgccgcctgg
     4321 catttcgaaa tggggcgaaa actcgcggcg ctgcgtgatg aaggaataat gttggtcgcc
     4381 agcggcaacg tggtgcataa cctgcgtaca gtgaagtggc acggtgatag ttcaccgtat
     4441 ccttgggcga tgtcgtttaa tgagtatgtg aaagcgaatc tgacgtggca agggccggta
     4501 gagcagcatc ctctggtgaa ttatctcgac catgaaggtg gcgcgttatc gaacccaacg
     4561 ccagagcact atctgccgtt gctgtatgtc ttaggtgcgt gggatgggca ggagccaatt
     4621 accattccgg tcgatggtat agaaatgggc agtttaagta tgctgtcggt gcagataggt
     4681 taacaattac cggatggcga atgccatccg gcatcattga ttattcaaca aaaatatgtg
     4741 gataaaaccg agacatatcc tgggtgatca atgcacggtc ttcacgaatg ccaattccgg
     4801 cgggttgatc gttcaccagc cagctaccaa tcagcatata gctgtcgccg aatttcggta
     4861 acgggtggaa ttgctgaaca atcatccctt cttcgccata cggaccttcc gctgcttcaa
     4921 tggttttgcc gttctcaatg atcgacacgt ttgcgccttc acgggagaag atcggtttaa
     4981 ccacatattt ttccatttgc ggatgatcat cttccgcaaa ataagcgggc agcaggttcg
     5041 ggtgattcgg gaacatctcc cacagtagcg gtagaagtgc cttgttggag ataatgctct
     5101 tccacgccgg ttccagccag cgtacgcctg catcctccag cttggtggaa aacatctcac
     5161 gcaacataaa ttcccacgga tacagcttga acaggttgga aattacctga tcctgtaaat
     5221 ccgtgaactg acctttttca cctaacccga tatcgtcaat gtagaggaac tcggtagcga
     5281 tttcagcttc cgtcgcgcag tcctgcaaat actgaatggt accgcgatct tccaccgtgt
     5341 cgcgacagca ggtgagatgc agcaactgga agccatactg ttcacgcagc tcaacgaagc
     5401 gatcgatcag tttttcttgc agactgttaa actggtcgct gccctccggc aagttaccgg
     5461 cgttaagctg atcttccagc cagatccact gaaagaacgc cgcctcgtat agtgacgttg
     5521 gcgtatcggc gttattttcc agaagtttag gttcgccagt gccatcccac gccagatcaa
     5581 gacgcgaata aagcgatggc tggtgcgtca gccatgactg gcgcacaaaa ctccaggtgt
     5641 gttttggaat gcggaatttg gtcatcagct catcgctggc gatcactttt tccaccactt
     5701 tcaggcacat ctggtgcagt tcggcggtga cttcttccag cttttcaacc tgggcgaggg
     5761 tcaacttgta gtaagcatct tcacaccagt acggctcgcc gtacatggtg tgaaaattga
     5821 aaccgtattc gtgggctttc tcacgccagt ccgggcgctc ggtaatactg actctttcca
     5881 tcggtatcag ccacccattg aacgagaaga ggtaccggtt gcgctacgct gcatagtgct
     5941 ttgtttggca acagattcac caaaaccgcc acgggtaacg gtagtggtgg tcgccggttt
     6001 tggtgccatg gctgtcttcg gtacggtcat ggtgcggcct ggctgggctg cgccatagtt
     6061 tttacccgtc gcgtcggtat atttaccgta agccgggctg gctgggtttt tcgaggagaa
     6121 cagcggctgc tgtgcaaatc ccgcaccgcc gcccatcaga cgacccatca tgtaaccggc
     6181 catcagcggc atccagaaac tcccgctgga ttgctgggcc tgcgcctggt tttctggtgc
     6241 catgccagcc tgggctggtg cctgctggca ctgaccttca ccaaattcag caacacagtc
     6301 ttcacgggtg gcgtatttcg gcgcagtacg ttcggcttct ttcagcgcat tgttgtacgc
     6361 ggtggtacat tcggcgcttt tgcctgggtt tgcagctgaa cagtcgtcag cattttgata
     6421 gagagacact gtttcatcac tcttttcaca gccagccagc ataaaaacag tggcaaccgc
     6481 gagagcgact ggtgtcagat ggcgtgcgct ccagtttttg cggaacgatg cgtggcgtat
     6541 ggattttgtc cgtttcattt ttgtcttccg ggacctgtgg taaataccca tcagaataga
     6601 ggatggctgg tcgaaattga agcgagaagg gggaagaatg cggcagataa cccgtatctt
     6661 tacgttgcct tacgttcaga cggggccgaa gccccgtcgt cgtcatcagt tacggaaagg
     6721 gttatgaccg ttactggtgg tagtgcgtgc ggatgtttgc tgaacgacgg gtgccgggct
     6781 atcaggcgca taaccatcag caatagcatt ctgttccggc gtttgcgggg caacgttttc
     6841 cggattagtg gaaaccggtt tgctcagcgc attgttcagt gccagcagat cctgctcgtt
     6901 caacgtaccc agggctgact taatattcag ctgattaatc aggtagttat aacgcgcatt
     6961 cgccagctct tgcttggcgt tgtacagcgt ggtggtcgca tccaacacat caacaatggt
     7021 acgcgtaccg accgagtagc ccgcttccat cgcgtctaat gagctttgag cggaaactac
     7081 ggcttgtttg taggcgttaa tactactgat agaagcatta atgttgttga aagaggaacg
     7141 tacggtctgc acgacgctac gatgcgcgct ttccagttgc tcgctggcac caacaaagtt
     7201 gtactgtgcc tgtttcacct gcgagttaac cattccgccc tgataaatcg gcagcgagaa
     7261 gctcaggcca actttgttct ggcccatatt gctatcgtca tactgggtac cagcggcacc
     7321 acgggttttc gaaccgctat aagaggtgtc agaaatcccg ctagaagccg ttaaatccag
     7381 agtcggtaag tgaccatcct gcgcctggcg aatttgctcg cgcgccaggt cctggctcaa
     7441 gcgtgcctgt aacagcgaca ggttgcgttt ttcggcttct ttcagcagcg cgttaaccgg
     7501 ctgtggtttg tcggttttaa agttttcgac attcagcgcc gccagttccg gatagtagtt
     7561 accggtgatc tggcgcagct gctctaccgc gttatcaagg ttattacgtg cggtcacttc
     7621 gttcgccagc acggtatcgt actgcgcgcg ggcgttctgc acgtcggtga tcgctaccag
     7681 gcccacgtta aaacgttggg tggtttgatc taattgacgg tagatcgctt ctttttgcgc
     7741 ctgtgtatag gaaagaacgt caatagcatt caacacgttg aaataagcgg tcgcggtgtt
     7801 gaggatcaag gtttgctgat cggtctgata cgtgacgtcc tgaatccctg ctgctttttc
     7861 ctgcagcgtt aacgcacgcc atttcgacat atcaaaaatg gattgagtta actgcaggga
     7921 cgcactggtc gcgttagagt tgatgccgtt cgcgtcgcgg tagccgttgc tataggtgta
     7981 atctgcacct aaacctagct gtggcagtaa tggactgcgc gcttcattaa ttttttcaaa
     8041 ggcagcatca cgatcggcgg cagacttacg caattccggg ttactaaggc gtgcttgctg
     8101 ataaacttgc atcaggttct cggcctggct caacgaactg aacccagaaa ggctcaggcc
     8161 gataagaatg gggagcaatt tcttcatttg cattccttgt ggtgaagcag tatttagcgc
     8221 gatcaaactg taaaattatt gcggattcta gcagaagccg ctaccgcaaa aggttggcgt
     8281 tacgtgccat agggcgttaa tgtgcctcaa tttaacacat gactgctcaa acggcagtca
     8341 aacgtcgctg aaattcacat ttaattcact attagtgcca ggacattaac aatgcttaag
     8401 ccagacaacc tgcccgttac atttggcaaa aacgatgtag aaattattgc acgagaaaca
     8461 ctttatcgcg gctttttttc attagatctt tatagatttc gtcatcgtct attcaacggg
     8521 caaatgagtc atgaggtacg gcgggaaatt tttgagcgcg gtcacgccgc agtcttgcta
     8581 ccctttgacc cagtgcgtga tgaagttgtg ctgattgagc agattcgaat tgccgcatac
     8641 gacactagcg aaaccccttg gctactggag atggttgccg gaatgattga agagggtgaa
     8701 agtgtggaag atgttgcccg tcgcgaagcg attgaagagg cggggctgat agtcaaacgg
     8761 accaaaccgg tgttaagttt cctggcaagc ccggggggca ccagtgagcg ttcgtcaatt
     8821 atggtgggcg aagtggacgc cacgaccgca agcggtattc acggtctggc tgatgaaaac
     8881 gaagatattc gcgttcatgt ggtaagccgg gaacaggcat accagtgggt agaagagggg
     8941 aaaatcgaca acgcagcgtc ggtcatcgct ttgcaatggc tgcagctgca tcatcaagcg
     9001 ttaaaaaatg agtgggcata aatgaagcgt tacacacctg actttcctga aatgatgcgc
     9061 ctgtgcgaga tgaacttttc acaattgcgc cgtttgttac cgcgcaatga cgcacccggc
     9121 gaaactgtaa gctatcaggt ggcaaacgca caatatcggc tgacgattgt ggaatcgacc
     9181 cgatacacta ccctggtgac aatagaacag actgcgcccg cgatcagtta ctggagcctt
     9241 ccgtcaatga cggtgcgtct gtatcatgac gcgatggtgg ctgaagtgtg ttcaagccag
     9301 cagatttttc gcttcaaagc gcggtatgat tatcctaata aaaagttgca tcaacgcgac
     9361 gaaaagcatc aaattaacca gtttttagcg gactggttgc gatactgttt agcacatgga
     9421 gcgatggcga ttccggttta ttagcgtcgt gaaacctaag gacaccattt ggaaagcctg
     9481 ttaacccttc ctctggctgg tgaggccaga gtcaggattt tacaaattac cgatactcac
     9541 ctgtttgcac aaaagcacga agccctctta ggggtaaaca cctgggaaag ctaccaggcg
     9601 gtgctggagg cgattcgtcc acaccagcac gaattcgacc tgattgtcgc gacaggtgat
     9661 ttagcgcagg atcaatcctc tgcggcctat cagcatttcg ctgaaggcat cgcaagtttt
     9721 cgtgcgccct gcgtctggct gccgggcaac cacgatttcc agcccgcgat gtacagcgcg
     9781 ttacaggatg cgggtatctc cccggcgaag cgggtgttta ttggtgagca atggcaaatc
     9841 ctgttgctgg atagccaggt gtttggcgtg ccgcacggtg agctaagcga gtttcagctt
     9901 gagtggctgg aacgtaaact ggctgatgcg ccagagcgcc atacgttgct gctgctgcat
     9961 catcatccgc tacctgcggg ttgtagttgg cttgatcaac acagcctgcg taacgcgggc
    10021 gaactggata ccgtgctggc gaagtttccg cacgtcaaat acttgctgtg cggtcatatt
    10081 catcaggagc tggatctcga ctggaacggt cgccgcctgc tggcaacacc gtcgacctgt
    10141 gtgcagttta agccgcactg ttccaacttt acgctggata ctatcgcgcc cggctggcgt
    10201 actctcgagt tacatgctga tggcacgctg accaccgagg tgcatcgcct ggcggacaca
    10261 cgtttccaac ctgataccgc ttcagaaggc tactgatgtc tacgcttctt tatttacacg
    10321 gtttcaacag ctcgccgcgc tctgcaaaag cgagcttgtt aaaaaactgg ctggcggaac
    10381 atcaccctga cgttgagatg atcattccgc agttgccgcc gtatccttcc gacgcggcag
    10441 agctgctgga atccattgtc ctggaacatg gcggcgattc gctgggtatt gtcggttcgt
    10501 cactgggggg atattacgcc acctggttgt cacaatgttt tatgctgccc gcagtggtgg
    10561 taaacccggc ggtgcgcccg tttgaactgc tgacggacta tctcggtcag aacgagaacc
    10621 cctacaccgg gcagcaatat gtgctagagt cacgccatat ttacgatctt aaagtcatgc
    10681 agattgaccc gctggaagcg ccggatttga tctggctgct gcaacagacg ggagatgaag
    10741 tgctggatta ccgccaggcg gtggcgtact acgcttcctg ccgccagact gtcatagaag
    10801 gcggcaacca cgcattcacg ggcttcgaag attatttcaa cccgatcgtc gattttcttg
    10861 gtctgcacca tctctgacga tcaactcgaa ttgctaactt aaaccatgac gcaaacttat
    10921 aacgctgatg ccattgaggt actcaccggg cttgagccgg ttcgccgccg tccggggatg
    10981 tataccgata ccactcgccc taaccatttg gggcaagaag tcattgataa cagtgtggat
    11041 gaagcactgg cgggtcacgc aaaacgcgtg gacgttattt tacatgctga ccagtcgtta
    11101 gaagttattg acgatgggcg cgggatgccg gtggatattc acccggaaga gggtgtaccg
    11161 gcggttgaac tgattctttg ccgtctgcat gcaggcggta aattctctaa caaaaattac
    11221 cagttctctg gcggcctgca tggcgtgggg atttcggtgg ttaacgccct gtcgaagcgc
    11281 gtagaagtta acgtgcgccg cgatggtcag gtttataaca tcgcctttga aaatggcgaa
    11341 aaggtgcagg atttacaggt tgtcggcaat tgcggtaaac gcaataccgg caccagcgtc
    11401 catttctggc cggatgaaac cttcttcgat agcccgcgtt tttctgtttc acgcctgacg
    11461 catgtgctga aagccaaagc agtactgtgc cccggcgttg agattacctt taaagatgag
    11521 atcaacaaca ccgaacagcg ctggtgctat caggacggtc tgaatgatta cctggcggaa
    11581 gcggtaaacg gtttaccgac gctgccagaa aaaccgttta tcggtaattt cgctggcgat
    11641 actgaagcgg tggactgggc gctactgtgg ctgccggaag gcggtgaact gctgaccgaa
    11701 agctacgtca acctgatccc aacgatgcag ggcggtaccc atgttaatgg cctgcgtcag
    11761 ggcctgctgg acgcgatgcg tgagttctgt gaataccgca acattttgcc gcgcggtgta
    11821 aagttgtcgg cggaagatat ctgggatcgc tgcgcctatg tgctgtcagt aaaaatgcag
    11881 gatccacagt ttgccgggca gaccaaagag cgtctctctt cgcgtcagtg tgcggcattc
    11941 gtttcgggcg tggtgaaaga tgccttcatc ctgtggctga accagaacgt tcaggcggcg
    12001 gagttgctgg cagagatggc gatttccagc gcccagcgtc gtatgcgtgc ggctaaaaaa
    12061 gtggtgcgta aaaagctgac cagcggcccg gcgctgcctg gcaaactggc tgattgtacc
    12121 gcgcaggacc ttaaccgtac cgaactgttc ctcgtggaag gtgactccgc aggcggatct
    12181 gccaagcagg cgcgcgatcg cgaatatcag gcgatcatgc cgctgaaagg taagatcctt
    12241 aacacctggg aagtttcttc cgacgaagtg ctggcctcgc aggaagtgca tgatatttcg
    12301 gtagcgatcg gtatcgatcc tgacagcgac gatctgagcc agcttcgtta tggcaaaatc
    12361 tgtatcctcg cggatgcgga ctctgatggt ttgcacattg ctacactgct ctgcgctttg
    12421 ttcgtaaaac acttccgcgc gttggtgaaa cacggtcacg tttacgtcgc actgccaccg
    12481 ctctaccgta ttgatctcgg gaaagaggtt tattacgcgc tgacggaaga agagaaagag
    12541 ggcgtacttg agcaattaaa acgcaagaaa ggcaagccga acgtccagcg ttttaaaggt
    12601 ctgggggaaa tgaacccgat gcaattgcgc gaaaccacgc ttgatccgaa cactcgccgt
    12661 ctggtgcagc tgactatcga tgatgaagac gatcagcgta ctgacgcgat gatggatatg
    12721 ctgctggcga agaaacgctc ggaagatcgc cgcaactggt tgcaagagaa aggcgacatg
    12781 gcggagattg aggtctgata atcctcccgc cagaagaata aaacatagcc cggtatacaa
    12841 gtgtataccg ggcttttttg atcaaaattc cattttcacc gtgaattgca cttcacgcgg
    12901 atcgccaatc tgattcccca gattatttgt ggcgatggaa gaggtgtaat acgttttatc
    12961 aaacaggttt ttgacgttta attgcagtgt gaccggatac tgcaatttca ttttgtatgc
    13021 ggcgaaggca tcggcaacga aatagcctgg cagataatag tcagccccat tggttgccga
    13081 acgacggctt acgccatgtc caccaccgcc aaacgtcagt gtgttattgc ctggcatgtt
    13141 atgaatgtca taggtcagga atagcgaacc ggtatgacga ggaacattcg gcaatggttt
    13201 ccctgcataa tcaggatctt ccagaacctt agcatcggta tagccgtagc tggcaatgat
    13261 attaatgttt tcagttaatg ctcccgcaag gtcgacttct acccctcttg aacgaacgcg
    13321 gcctgccgtt ttggcgatgg tttcatcacc aatactttcg gtatacaaca cgttacgttt
    13381 atggatatca aacagcgcaa tatctgcggt tataccatcg aacagctcga attttgcccc
    13441 gacttcgtaa gcattagatg attccggtgg aagatctccg atgtagctgg caattgacga
    13501 ttgcggcata aatgtttgcg aataattagc aaataaggat accgatggcg tcagtttgta
    13561 gactaacccc agtttgggcg tccattgttc atcgcggctg tcagtattga cattaaaagg
    13621 acggccttta cccgcatact gcgtgtaata ctgatagcgg atcccggcga cggcaatcca
    13681 gttatcggtc agatagagcg catcctgtgc ataagctgag tagctctcct gtttgatcgt
    13741 ctgatcactg tccgacgccg aaaccgttgt gcatttgctg gtattgccat aaacggggtt
    13801 gtagatattg aaatctttag cgtttttaca gcgaatcata tctgtgcgca gaagatcata
    13861 atattcatat gacaccccac ccagaatctc attatagaac ccagcaatat caacattccc
    13921 ttgcagatcc gcacgagtag aatgcatacg ctgggtagat ccctgagttg catcaacacg
    13981 ccgtgtcagc gttcctgtcg ttgcatcata cgcggtaacg cgagcctgat tgtcgctgta
    14041 tttatcctgg ctgtagctgt aatcaaagcg cgctgtccac tggctattga gatgatattc
    14101 tgcgttgagt tgcgccagat cggactgacc atctgtaata ttaaacggtt cgtcaaaacg
    14161 tatttttcga tcaacgttta cgggctgttt cgtcgtaagg tcgaaaatcg ttccacgatc
    14221 gaacggagtt ttatagtccc gatgggaata gagcatggtt actgttgcat tatcaccaaa
    14281 ccaggtgagt gacggggcaa taaatgtact gcgctcttta ccgaagtttc gccagtaatc
    14341 ttcatcctgc acttcccccg tcaggcggta tgccagctga gtgccttcaa tgggacctgt
    14401 gatatcaagt tgcccagtgc ctccgccaaa actggaggac gtggctgaga cagaaccatg
    14461 gaatgttttt tccgggcgct tggtcacgac gttaatcaat ccaccaggat cgagaatgcc
    14521 atacagcgtg gaggccggac cttttagcac ttccacacgt tctgtggcgg cgttgaaact
    14581 gcgaggaagt acagttcgca ggccgttggt catgatggag ccatcccggt tagcaccaaa
    14641 accacgacgt acaaaggcgt cctgagttcc gcctaatgta ttggtctgta ccacgttact
    14701 gacgttataa agcgcttcat ccagcgtcgt tgcattctga ttttccagaa cctgatcgct
    14761 aaccgtattg accacctgcg ggatatccag catcggcata tcggttaatg tcgccgtgga
    14821 agtgctcaga ggttgataac catcggtcgc ctcagttgcg gtatttgcat ccgctgtaac
    14881 agtgattgtt tcgccatctt tggtcgttgc agtgtcggtt gcatgaatca ttggtgctgc
    14941 aaagagcagt gaaaagagcg cccgaccttt gattcctgag aatgaaggtg tgaacttagc
    15001 catttagtct gttatcccaa aaactcacgg attgtcgtga gtgaaaattg tttcttgttg
    15061 ctcactaagc actatccgtt tgttatttta ccatcggata tgtaattgag aatc
//
LOCUS       DBFIFS010000107        15101 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.107, whole genome shotgun sequence.
ACCESSION   DBFIFS010000107 DBFIFS010000000
VERSION     DBFIFS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.107"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(100..2379)
                     /gene="maeB"
                     /locus_tag="N7A07_002859"
     CDS             complement(100..2379)
                     /gene="maeB"
                     /locus_tag="N7A07_002859"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU2477488.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2668..3618
                     /gene="tal"
                     /locus_tag="N7A07_002860"
     CDS             2668..3618
                     /gene="tal"
                     /locus_tag="N7A07_002860"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU2477489.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3638..5641
                     /gene="tkt"
                     /locus_tag="N7A07_002861"
     CDS             3638..5641
                     /gene="tkt"
                     /locus_tag="N7A07_002861"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU2477490.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALVRQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5736..6779)
                     /gene="ypfG"
                     /locus_tag="N7A07_002862"
     CDS             complement(5736..6779)
                     /gene="ypfG"
                     /locus_tag="N7A07_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU2477491.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6905..7480)
                     /gene="nudK"
                     /locus_tag="N7A07_002863"
     CDS             complement(6905..7480)
                     /gene="nudK"
                     /locus_tag="N7A07_002863"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU2477492.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(7548..9527)
                     /gene="aegA"
                     /locus_tag="N7A07_002864"
     CDS             complement(7548..9527)
                     /gene="aegA"
                     /locus_tag="N7A07_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU2477493.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9733..11433
                     /gene="narQ"
                     /locus_tag="N7A07_002865"
     CDS             9733..11433
                     /gene="narQ"
                     /locus_tag="N7A07_002865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU2477494.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11597..14710
                     /gene="acrD"
                     /locus_tag="N7A07_002866"
     CDS             11597..14710
                     /gene="acrD"
                     /locus_tag="N7A07_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU2477495.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14809..14868)
                     /gene="ypfM"
                     /locus_tag="N7A07_002867"
     CDS             complement(14809..14868)
                     /gene="ypfM"
                     /locus_tag="N7A07_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU2477496.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3549 a         3994 c         3931 g         3627 t
ORIGIN      
        1 tttttattat tcgcaaactc agtaataagc tttactaata tattgcagct aaaccgctta
       61 ccctgaatat tcagggtaag cgtgagagtt aaaaaagaat tacagcggtt gggtttgcgc
      121 ttcaaccacg gccagcgcca ccatgttgac gatacgacgc accgatgcga tcggcgttaa
      181 cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac cttccgagct
      241 ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca tcaccagaat
      301 attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg cttccaccag
      361 cgctgcatcg ccgtgcattt caccatcaat catcagttct ggcgcacgtt ccctgaccag
      421 ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa agttggagtg
      481 cgacaacaga gcaacgcgcg gttcaatacc gaaacgacgg acagtttctg ccgccatcaa
      541 ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat cggcaataaa
      601 ggtgttacca ctcggcagca gcagcgcgtt cattgcacct gcggtgtgaa cgccatcgcg
      661 ataaccaaag acatttttca ccacgctaaa atgttcatga taatcaccca ccgtaccgca
      721 aatcattgca tcggcttcgc cacgctgaac catgatcgcg ccgatcactg tcgggttact
      781 gatcagcgcc cgctgcgcct gttcctgagt gacgccgcga cgcttcatga tctggaagta
      841 ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgatct caaaatcaac
      901 gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc
      961 gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtggcat gcagaacgcg
     1021 cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct gggagaaaat
     1081 cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga tgtagacgtc
     1141 gaaatcagca atcggacgcg tcgccacacc cgactccatc gccgctttcg cgaccgcagg
     1201 agcaatcttg actatcagac gcggatcaaa cggttttgga atgatgtatt cagggccaaa
     1261 gctcagatcc tgatcgccat acgctgaagc caccacttca ctctgttccg catgggccag
     1321 ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg ttgcgccaac
     1381 gtccagcgcg ccacggaaga tgaacgggaa gcaaaggacg ttgttcacct ggttcgggta
     1441 gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca gcggcggcag
     1501 aatttccggt tccgggttcg ccagcgccag gatcattggc gctcgagcca ttttcttcac
     1561 catttcctgg gtcagcactt tcgggccgga acagccgagg aaaatatctg cgccttcaat
     1621 cacatcatcg agggtacgtt tgccgtcatc cactaccgca tatgcggctt tggtttccgc
     1681 catgtttggc tcacggccct gatagataac gccttttgaa tcgcaaacca cgatgttatg
     1741 tttttgcaga cccaacgcta ccagcaggtt catacaggcg attgctgcag cacccgcgcc
     1801 ggaaaccacc atacgcacgt cagagatgtt tttctccacc acgcgcaagc cgttgaggat
     1861 ggcggcagtg ctgataattg ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat
     1921 ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt cttcgaggtt
     1981 gatgccgccg aaggtcggtt cgagcgcagc gacgacttca ataaatttgt ccgggtcgag
     2041 ttcatcaact tcaatgtcaa atacatcaat cccagcgaat ttcttaaaca gaacgccctt
     2101 gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca gcaccgccgt
     2161 accgttagag atcaccgcca ccaggttacc gcgcgcggta tatttgtagg cttttaacgg
     2221 gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca gcgccagatc
     2281 gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa ctggaaattc
     2341 atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc tttcacgtaa
     2401 cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc acattattac
     2461 cttgccggag tctacagact ttgagcaagt ccaaactctc accattaata taatgttttg
     2521 gtaataatcc tataacactg atgttacctg cttaatccag caataccatg cctgtctgct
     2581 atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc actcatctaa
     2641 cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa acagttcacc
     2701 actgtcgtgg cagacagcgg cgatattgag tccattcgcc attatcatcc ccaggatgcc
     2761 accaccaatc cttcgctgtt actcaaggct gccggattat cacaatatga gcatttaata
     2821 gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca
     2881 gcgtgtgaca aactggcggt caatttcggt gctgaaatcc tcaaaatcgt acccggtcgc
     2941 gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat tgagaaggcg
     3001 cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat tctgatcaaa
     3061 ctggcttcga cctgggaagg aattcgcgcg gcagaagagc tggaaaaaga aggtattaac
     3121 tgcaacctga cgctgctgtt ttcttttgca caggcgcggg cctgtgcaga agcgggcgtt
     3181 tttctgattt cgccgtttgt cgggcgtatt tatgactggt atcaggcacg caagccgatg
     3241 gacccatatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat ctacgactac
     3301 tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg caccgaacaa
     3361 atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgct gaaggagctg
     3421 caggaaaaag tttcgccagt ggtacgtaaa ttaatcccac cttctcagac gttcccacgc
     3481 ccagctccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc gatggcggta
     3541 gaaaaactgt ctgaaggcat tcgtctgttc gccgttgatc aacgcaaact ggaagatctt
     3601 cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag accttgccaa
     3661 tgcgattcgc gcactcagta tggatgcggt acaaaaagcc aattctggtc atcccggcgc
     3721 gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta aacataaccc
     3781 taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc acgcgtcgat
     3841 gctgctctac agtttgctgc atctgaccgg ttacgacctg ccgctggaag agctgaagaa
     3901 ctttcgtcaa ctgcattcga aaacccctgg ccacccggaa atcggctata cgcccggagt
     3961 tgaaaccacc accggtcctc ttggacaagg tttggcgaac gccgtcgggc tggcgatagc
     4021 ggaacgtaca ctggcggcgc agtttaacca gccggatcat gaaattgtcg atcacttcac
     4081 ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag tctgttcgct
     4141 ggcgggcacg ctgggactgg gcaagctgat tggtttttac gatcacaacg gtatttcgat
     4201 tgatggagaa accgaaggct ggtttaccga cgatacggca aaacgttttg aagcctatca
     4261 ctggcatgtg atccatgaaa tcgacggtca cgatccgcag gcggtgaagg aagcaatcct
     4321 tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg tgattggctt
     4381 tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccgctgg gggaagaaga
     4441 agtggcgctg gtacggcaaa aactgggctg gcaccatccg ccatttgaga tccctaaaga
     4501 tatttatcac gcttgggatg cccgcgaaaa aggcgaaaaa gcgcagcaga gctggaatga
     4561 gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagttta cccgtcggat
     4621 gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca atgagttgca
     4681 ggcgaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg
     4741 gccgatgcta ccggagctgc tcggcggttc ggcggatctg gctcccagca acctgaccat
     4801 ctggaaaggt tctgtttcgc tgaaggaaga tccggcgggc aactacattc actacggggt
     4861 gcgtgaattt ggcatgaccg ctatcgccaa cggcattgcg caccacggcg gctttgtgcc
     4921 gtataccgca acgttcctga tgtttgttga atacgcccgt aacgccgcgc ggatggcggc
     4981 actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggtc tgggcgaaga
     5041 tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc caaatttcag
     5101 cacctggcga ccgtgcgatc aggtggaagc ggcggtgggc tggaagctgg cggttgagcg
     5161 ccacaacgga ccgacggcac tgattctctc caggcagaat ctggcccagg tggaacgtac
     5221 gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa
     5281 gccagatatt attttgattg ccaccggttc agagatggaa atcaccctgc aagcggcgga
     5341 gaaattagcg ggagaaggtc gcaatgttcg cgtggtttcc ctgccctcga ccgatatttt
     5401 cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt
     5461 ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga aaggggcaat
     5521 tgtcgggatg acgggttatg gggaatctgc tccggcggat aagctgttcc cgttctttgg
     5581 ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtaa aaggtgcctg
     5641 atggtgatcg ccggatgctg attgccggat gcgacgctga cgcgtcttat ccggcctaca
     5701 gcgtcttatc cggcctacat gtcccgccat tttgtttaac gggtgatcca caacgtgggc
     5761 caggcgtctg gcccatgcca gttatcgcag gtgggttctg cggcgtaacg caccaggcga
     5821 aaacgctgac cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct
     5881 ttcgctaagg tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt
     5941 tcattcgtct cctgaccgtt gttgaacggc aaacgcagcc ggaccgggcg cgaggccagc
     6001 ggctttttac gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag
     6061 ctaatcatca tcagcgcttt atcatcagtc agcgcagtga cattcacctc acgacgcaat
     6121 ggatcaagcg agcagcgcag accattcatc cgccagtttc cataatccag caaatcgttg
     6181 cgttcttcga gtgagagtgg cgtcggcgtt gggttaacca ccacgacctc tttcagcgca
     6241 ggcgcgggcg gtacgctgag cggcggttcg tcccctttct tgatccacgc ggtttcactg
     6301 ccaacgcgct tttgctgagc atcaataaac aacaacgccg cttttaagtc actcagagaa
     6361 atggtctgat tgccgtcgcg taaggtgatc gccttccctt cctgaatcat ctgcaaaaac
     6421 gcggtgatgg ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca
     6481 ccacttaacg ccaacggctc gccatctaac agcagccgtg gcgcaatttc cccttcagac
     6541 gcatccggcg acttcaaccc gccgcgctca atacgtaaaa cggcatcggt atgcgccccg
     6601 gcgctgcggc tcagggtcat caccagtcca ttatgatcgc ccgtattacg cgccacgcag
     6661 aagttttggt tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc
     6721 cacaccaaag acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg
     6781 gacggtacga ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta
     6841 gcgcggctca atcggatttg tcggattgag ccaagaataa acaacgaaat attattgtta
     6901 tttttcagtc tattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt
     6961 cacgtatctc gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt
     7021 caatatcttc atcttcgaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg
     7081 cgataaaaaa gtggattagc tcagtcacac cgcctggcga catatacagt tcaaataatt
     7141 tgcgcacttc gcccacttca tagcccgtct cttcaatcgc ttctttgcga atgcacactt
     7201 ccggttcgtc gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc
     7261 cattaaccca ggtagcgaca cggaactgac gaatcagaac cacggtcttt ttcttcgcgt
     7321 tgtacaggag gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa
     7381 cttcgccatc tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg
     7441 agagaatttt gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa
     7501 tgaacaatta tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg
     7561 actgatttta cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt
     7621 gcggtaacca ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg
     7681 gtggtctggt aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc
     7741 gttacaccgt gcgactccag ccacggcatc ccgtgcggat tgaagccaaa cgccataatc
     7801 accgcgtcgg ctggcatgac aaattcactg ccttccaccg gcactggacg ccgacgcccc
     7861 tgggcatccg gctcgccaag acgcgtgcgc aggaaacgaa tcccgcagac gtgaccttgt
     7921 tcattcagct caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg
     7981 gcgttcttca cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag
     8041 gtgacgttac tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg
     8101 ccgcccagta ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct
     8161 tcgagcccca tcacctgttt agtgttggcg atgaggaacg gcagcgcgtc ataaacgccc
     8221 ggcgcatctt cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag
     8281 accgcgtcgt attgttccaa aagcgaatcc aaagagacat ctttacccac ttcacaattg
     8341 agttcgaagt gaatccccat cgcgctgaag atttcccgac gacgtgccag cagggattta
     8401 tccagtttga aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg
     8461 tacaccgtca ccgccacgcc attgcggatc agaacatccg cacaggccag ccctgccgga
     8521 cctgcaccga taatcgccac ccgcttgtcc actttggtga catggcttaa gtcaggacgc
     8581 caacctttcg ccaacgcctg atctgaaatg tagcgttcaa tgttgccgat agttaccgca
     8641 ccgtgctcat cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt
     8701 ccggtaattt ccggcagggt gttggtcagg tgagaaagct cgacggcggc gtcgatgttt
     8761 ccggctttca ccagttcaat ccactgcggt atatggttat gcagcgggca ggtccattca
     8821 caaacgctgt gctcgccgca tttaaggcag cgcgaggctt cccgttgtgc ctgatcggca
     8881 cggaatggca gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc
     8941 ggctcgccac gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct
     9001 tgtgcagcgg tactggcatg ccacggttga tgttcctgac gtgcggtacg caagcggcgg
     9061 gatttcgcca tgcctgagag tgcggcgtca gtgaccagct gcagcgcgtc cgccgggcaa
     9121 ttctcgacac aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg
     9181 gctttaactt tgcccgctgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag
     9241 gccaccacgc aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca
     9301 acgtggctga ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga
     9361 caggtcactg cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg
     9421 tgttggctca ggacatgttg ctcatcattg tgagccatga cacaagcgat ttcacaagca
     9481 tgacaaccca gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt
     9541 tttggttgta aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa
     9601 tcggcaatgt tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa
     9661 ataatcccat cactaactaa attgcgtttc agcgaactgg aacattaatg attttttgtg
     9721 gagaagacgc gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt
     9781 tacattgtgc tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt
     9841 ttgcgcgacg ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg
     9901 ggctacgact tgcaaagtgg cactccacaa ctcaatgcac atcgccagct gtttcagcag
     9961 gcactgcatt caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa
    10021 actcgctatg cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc
    10081 gatttgccgt ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg
    10141 ctggctttac agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct
    10201 ggcggtatcg gtattttcac gctggtcttt tttactttgg gccgcatacg ccatcaggtg
    10261 gttgccccgc tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg acagttcgac
    10321 tcgccaccgc tggataccag cctgccgaat gagcttggtc tacttgcaaa aacctttaac
    10381 cagatgtcga gcgagctgca taaattgtac cgttcgctgg aggcgtcagt agaagaaaag
    10441 acccgcgatc tccacgaggc caagcgtcgt ctggaggtgt tgtatcaatg ttcgcaggcg
    10501 ctgaacacca gccagattga tgtgcattgt ttccgccata ttttgcagat tgttcgcgac
    10561 aatgaagcgg ctgaatatct ggagttaaat gtcggtgaaa actggcggat tagcgaaggg
    10621 caaccaaacc cggaattgcc gatgcagatt ttaccggtga caatgcaaga gacggtttac
    10681 ggcgaactgc actggcaaaa tagtcacgtt tcatcatcag aaccgctgct taacagcgtt
    10741 tcgtcgatgc tgggacgcgg tttgtacttt aatcaggcgc agaagcattt tcagcagtta
    10801 ttgttgatgg aagaacgtgc aaccatcgcc cgcgaattgc acgactcgct ggctcaggta
    10861 ctttcttact tacgtatcca gttgacgtta cttaagcgtt cgataccgga agataacgcc
    10921 accgcacaaa gtatcatggc cgatttttcc caggcgttga atgatgctta tcggcagtta
    10981 cgcgagctgt tgactacttt tcgcctgacg ctgcagcagg cggatctccc ctccgcgttg
    11041 agggaaatgc tggatacgtt acaaaatcaa accagcgcca aactgaccct cgactgccgt
    11101 ctgccaaccc tggcactgga tgcgcaaatg caggtgcatt tgttgcaaat tattcgcgaa
    11161 gcggtgctga atgcgatgaa gcacgccaac gccagcgaaa tcgccgtcag ttgcgtcacc
    11221 gcgccggacg gcaatcacac ggtttatatc cgtgataacg ggattggtat cggtgaaccg
    11281 aaagaacccg aaggtcatta tggtctgaat atcatgcgcg aacgcgcgga acggctaggt
    11341 gggacgctga ctttttcaca accttccggc ggcggcacgt tagtgagtat tagctttcgc
    11401 tctgcggagg gtgaggaaag tcagttgatg taatgcctcc tactgaccaa agaatacttg
    11461 cagttaaggt tcagtataaa agggcatgat aatttacatt aactcctttt tttctccacg
    11521 attggctcgt accttgccgc tacagtgaag caagtcaagc ctacaacgat acgcagaaac
    11581 acgaggtcct cttttaatgg cgaatttctt tattgatcgc cccatttttg cctgggtgct
    11641 ggcaatcctg ttgtgtctga caggtaccct ggcgattttt tcattgcccg ttgaacaata
    11701 ccccgatctc gcgccaccga atgtgcgagt gaccgctaac tatcccggcg catcggccca
    11761 gacgctggaa aacaccgtga cccaggttat cgagcaaaat atgaccggcc tcgataatct
    11821 catgtatatg tcatctcaga gcagtggcac cggtcaggca tctgtcactt taagttttaa
    11881 agcaggcacc gatccggacg aagccgtgca gcaagtacaa aaccagctgc aatcagccat
    11941 gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgact gtgcgtaaaa ccggcgatac
    12001 caacattctg accattgcct tcgtctctac cgatggttcg atggataaac aggatattgc
    12061 tgattatgtt gccagtaata ttcaggaccc gttaagccgc gtaaatggcg tcggggatat
    12121 cgatgcctat ggttcgcaat attccatgcg tatctggctg gacccggcga aactcaacag
    12181 tttccagatg acagctaaag atgtcactga tgccattgag tcacagaacg cgcagattgc
    12241 ggttgggcaa cttggtggta caccttccgt cgataagcag gcgctcaacg ccaccattaa
    12301 cgcccagtca ctgctgcaaa caccagaaca gttccgcgat atcaccttgc gggtgaatca
    12361 ggacggctca gaagtaaggc tcggcgatgt cgccaccgcc gaaatggggg cggagaaata
    12421 cgattatctt agccgcttca atggtaagcc agcctccggg ttgggggtaa aactggcctc
    12481 cggcgcgaac gaaatggcga cagcggagct ggtgctcaat cgtctcgacg agctggcgca
    12541 gtatttcccg cacggactgg aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc
    12601 ctccattgaa gacgtggtga aaacgctgct ggaagctatc gctctggttt tcctcgttat
    12661 gtatctgttc ctgcaaaact tccgcgccac gctgataccc actatcgccg tgccggtggt
    12721 gttgatggga accttctccg tactttacgc cttcggttac agcgtcaaca ccttaaccat
    12781 gttcgcgatg gtgctggcga tcggtctgct ggtggatgac gccatcgtgg tggtggaaaa
    12841 cgtcgaacgt attatgagtg aggaaggact cactcctcgc gaagccacgc gtaaatcgat
    12901 ggggcagatc cagggggcac tggtcgggat tgcgatggtg ctttcggcgg tatttgtacc
    12961 aatggccttc ttcggcggca ccaccggtgc catctatcgc cagttctcta ttaccattgt
    13021 tgcggcgatg gtgctgtcag tactggtagc gatgatcctc actccggctc tgtgtgccac
    13081 actacttaag ccactgaaaa aaggtgagca tcacgggcaa aaaggctttt ttgcctggtt
    13141 taaccagatg tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga aaattctcca
    13201 ccgtagcctg cgctggattg tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt
    13261 cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgt ggcatgttta ctacctcggt
    13321 acaattgccc agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa
    13381 atattacttc acccatgaaa aagacaacat catgtcggtg tttgccactg ttggttctgg
    13441 ccctgggggt aacgggcaaa acgtggcgcg aatgtttatc cgcctgaaag actggagcga
    13501 acgcgacagt aagaccggca cctcgtttgc cattatcgag cgtgcaacaa aggcgtttaa
    13561 ccaaattaaa gaagctcgcg ttatcgccag cagcccgcca gcaattagcg gtcttggtag
    13621 ttctgcaggt tttgatatgg agttgcagga ccacgctgga gcaggtcacg atgcgctgat
    13681 ggcagcacgt aaccagttgc tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg
    13741 ccataacggc ctcgatgaca gtccgcagtt gcagattgat atcgaccagc gtaaagctca
    13801 ggcgctgggc gttgctatcg acgatattaa cgacacactg caaaccgcct ggggttcgag
    13861 ctatgtgaat gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc
    13921 gccgtatcgc atgctgccag atgacatcaa tctctggtat gtccgaaata aagatggcgg
    13981 catggtgccc ttctctgctt tcgcgacctc acgctgggag acaggctcgc cgcgtctgga
    14041 acgctataac ggttattctg cggttgaaat tgttggggaa gccgcaccgg gggtcagtac
    14101 cggtacggcg atggatatta tggaatcgtt agtgaagcag ctaccaaacg gctttggtct
    14161 ggagtggacg gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc cggcgctgta
    14221 cgccatttcc ttgctggtgg tattcctgtg tctggctgca ttgtatgaaa gctggtcggt
    14281 accgttctcg gtaatgctgg tcgtgccgct gggggtaatc ggcgcgctgc tggcaacctg
    14341 gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg tcattggttt
    14401 atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca
    14461 cgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac
    14521 ctcgctggca tttatcttcg gcgtattgcc aatggcaacc agcacgggtg ccggttccgg
    14581 tggtcagcat gcggtgggta ctggcgtaat gggcgggatg atttcagcca ctattctggc
    14641 tatttacttc gtgccgctgt tctttgtgct ggtgcgccgc cgcttcccgc tgaagccgcg
    14701 cccggaataa gcaataaaaa aggcgacatg ccaatgtgtc gcctttttca actttccgat
    14761 taagaacctg ctcagcgggt tcttgctgtt tgtactttgt ctcaggaatt acttacgaag
    14821 cataacttca ataaagtctt tccagttccc cagttcacgt tcaatcataa caacctctct
    14881 tataattatg ggtattctac ggaaacaata taccgtggtg aagctaattt actcgattgc
    14941 tgcgatgact acctccgggg gacaaacctt atgtaaatac tatggtcctg cagtgatgat
    15001 ttgtatgtga tacacagcaa catttcgaga tattcatacg gcatctaata ctgatttaat
    15061 tctggttaaa atacagacag ataacaagat gaatattctt a
//
LOCUS       DBFIFS010000108        14969 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.108, whole genome shotgun sequence.
ACCESSION   DBFIFS010000108 DBFIFS010000000
VERSION     DBFIFS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.108"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..215
                     /locus_tag="N7A07_002868"
     CDS             <1..215
                     /locus_tag="N7A07_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU2477497.1"
                     /translation="AKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKM
                     ETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(335..514)
                     /gene="yoaH"
                     /locus_tag="N7A07_002869"
     CDS             complement(335..514)
                     /gene="yoaH"
                     /locus_tag="N7A07_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU2477498.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            588..1949
                     /gene="pabB"
                     /locus_tag="N7A07_002870"
     CDS             588..1949
                     /gene="pabB"
                     /locus_tag="N7A07_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU2477499.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1953..2531
                     /gene="nudL"
                     /locus_tag="N7A07_002871"
     CDS             1953..2531
                     /gene="nudL"
                     /locus_tag="N7A07_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU2477500.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2715..4079
                     /gene="sdaA"
                     /locus_tag="N7A07_002872"
     CDS             2715..4079
                     /gene="sdaA"
                     /locus_tag="N7A07_002872"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU2477501.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4210..5808
                     /gene="pdeD"
                     /locus_tag="N7A07_002873"
     CDS             4210..5808
                     /gene="pdeD"
                     /locus_tag="N7A07_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2477502.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5812..7368)
                     /gene="yoaE"
                     /locus_tag="N7A07_002874"
     CDS             complement(5812..7368)
                     /gene="yoaE"
                     /locus_tag="N7A07_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU2477503.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7356..7514)
                     /gene="yoaL"
                     /locus_tag="N7A07_002875"
     CDS             complement(7356..7514)
                     /gene="yoaL"
                     /locus_tag="N7A07_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU2477504.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7831..8802
                     /gene="manX"
                     /locus_tag="N7A07_002876"
     CDS             7831..8802
                     /gene="manX"
                     /locus_tag="N7A07_002876"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU2477505.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8865..9665
                     /gene="manY"
                     /locus_tag="N7A07_002877"
     CDS             8865..9665
                     /gene="manY"
                     /locus_tag="N7A07_002877"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU2477506.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9678..10529
                     /gene="manZ"
                     /locus_tag="N7A07_002878"
     CDS             9678..10529
                     /gene="manZ"
                     /locus_tag="N7A07_002878"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU2477507.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10584..11042
                     /gene="yobD"
                     /locus_tag="N7A07_002879"
     CDS             10584..11042
                     /gene="yobD"
                     /locus_tag="N7A07_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU2477508.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
     gene            11353..11475
                     /locus_tag="N7A07_002880"
     CDS             11353..11475
                     /locus_tag="N7A07_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477509.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            11472..12038
                     /gene="mntP"
                     /locus_tag="N7A07_002881"
     CDS             11472..12038
                     /gene="mntP"
                     /locus_tag="N7A07_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU2477510.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12035..12844)
                     /gene="rlmA"
                     /locus_tag="N7A07_002882"
     CDS             complement(12035..12844)
                     /gene="rlmA"
                     /locus_tag="N7A07_002882"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU2477511.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVLDCQTDFNIHLWQRSY"
     gene            complement(13010..13219)
                     /gene="cspE"
                     /locus_tag="N7A07_002883"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13010..13219)
                     /gene="cspE"
                     /locus_tag="N7A07_002883"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU2477512.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13232..13375)
                     /gene="yobF"
                     /locus_tag="N7A07_002884"
     CDS             complement(13232..13375)
                     /gene="yobF"
                     /locus_tag="N7A07_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU2477513.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(14043..14330)
                     /gene="yebO"
                     /locus_tag="N7A07_002885"
     CDS             complement(14043..14330)
                     /gene="yebO"
                     /locus_tag="N7A07_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU2477514.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(14405..14548)
                     /gene="mgrB"
                     /locus_tag="N7A07_002886"
     CDS             complement(14405..14548)
                     /gene="mgrB"
                     /locus_tag="N7A07_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU2477515.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14708..14947
                     /gene="yobH"
                     /locus_tag="N7A07_002887"
     CDS             14708..14947
                     /gene="yobH"
                     /locus_tag="N7A07_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU2477516.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
BASE COUNT         3800 a         3698 c         3650 g         3821 t
ORIGIN      
        1 cggcgaaggg aatctttaaa tatttaaaag aactgggagt acccgcgagt gccgctgata
       61 ttacggcgcg agctgaccag gaaggctgga accctgggtt cacggaaaaa atggttggat
      121 gggcaaaaaa aatggagaca ggtgaacgct ctgtgattaa aaatcctgag tacttttcaa
      181 catatatgca ggaagaacta aaagcactgg tctgagttaa atttatatca gcataaatgg
      241 gtcaggacgc ttttaatcac atataaaaag cgtccttttt ctcacaatca aataatcatc
      301 agcttctata acgaaatcga tacggattag tgtgttactc gtcttcatcc tcaaaacgcg
      361 ccacgattcg ctcgccggag tggttggcac gcagctcttc tgccaccagc gcgattgcct
      421 ggccgctgct catcccttgt gccatcagtt cctggatccg ctcgacagct ttttgctgct
      481 gttcatgggt gagtgaaggt aaacctgcaa acattgttaa ctcctgctaa attgttggcg
      541 ctaattattt catgctaccc ggcacatagc cagtagagtc aggactgatg aagacgttat
      601 ctcccgctgt gattacttta ccctggcgtc aggacgccgc tgaattttat ttctcccgct
      661 taagccacct gccgtgggcg atgcttttac actccggcta tgccgatcat ccgtatagcc
      721 gctttgatat tgtggtcgcc gatccgattt gcactttaac cactttaggt aaagaaaccg
      781 ttgttagtga aagcgaaaaa cgcacaacga ccactgatga cccgctacag gtgctccagc
      841 aggtgctgga tcgcgcagac attcgcccaa cgcataacga agatttgcca tttcagggcg
      901 gcgcactggg gttgtttggc tacgatctgg gccgccgttt tgagtcactg ccagaaattg
      961 cggaacaaga tatcgttctg ccggatatgg cagtgggtat ctacgattgg gcgctcattg
     1021 tcgaccacca gcgtcataca gtttctttgc tgagtcataa tgatgtcaat gcccgtcggg
     1081 cctggctgga aagccagcaa ttctcaccgc aggaagattt cacgctcact tccgactggc
     1141 aatccaatat gacccgcgag cagtacggcg aaaaatttcg ccgggtacag gaatatctgc
     1201 acagcggtga ttgctatcag gtgaatctcg cccagcgttt tcatgcgacc tattctggcg
     1261 atgaatggca ggcattcctt cagcttaatc aggccaaccg cgcgccattt agcgcttttt
     1321 tacgtcttga acagggtgca attttaagcc tttcgccaga gcggtttatt ctttgtgata
     1381 atagtgaaat ccagacccgc ccgattaaag gcacgctacc acgcctgccc gctcctcagg
     1441 aagatagcaa acaagcagaa aaactggcga actcagcgaa agatcgtgcc gaaaatctga
     1501 tgattgtcga tttaatgcgt aatgatatcg gtcgtgttgc cgtagcaggt tcggtaaaag
     1561 taccagagct gttcgtggtg gaacccttcc ctgccgtgca tcatctggtc agcaccataa
     1621 cggcgcaact accagaacag ttacacgcca gcgatctgct gcgcgcagct tttcctggtg
     1681 gctcaataac cggggctccg aaagtacggg ctatggaaat tatcgacgaa ctggaaccgc
     1741 agcgacgtaa tgcctggtgc ggcagcattg gctatttgag cttttgcggc aacatggata
     1801 ccagcattac tatccgcacg ctgactgcca ttaacggaca aatatactgc tctgcgggag
     1861 gtggaattgt cgccgatagc caggaagaag cggaatatca ggaaactttt gataaagtta
     1921 ataagatatt acgccaactg gagaagtaag acgtggaata ccgtagcctg acgcttgatg
     1981 attttttatc gcgctttcaa cttttgcgcc cgcaaattaa ccgggaaacc ctaaatcatc
     2041 gtcaggctgc cgtgttaatc cccatcgtcc gtcgaccgca accggggttg ttgctgactc
     2101 agcgttcaat tcatctgcgt aaacacgctg gacaagtggc attccctgga ggtgcagtcg
     2161 atgacacgga cgcatcagtt atcgccgccg cgctgcgcga agctgaagaa gaggttgcta
     2221 taccgccttc cgccgttgaa gttatcggcg tgctgccgcc cgtcgatagc gtaactggct
     2281 accaggtaac cccagtggtc ggcattatcc cgcccgatct gccgtatcgc gccagtgaag
     2341 atgaagtttc cgctgtgttt gaaatgccgc tcgcccaggc attacatctg ggtcgttatc
     2401 accctttaga tatctaccgc cgcggcgatt cacatcgggt atggctatcc tggtacgaac
     2461 agtattttgt atggggaatg accgcaggca taattcgtga gctggcgctg caaattggtg
     2521 tgaaaccctg actatactta tctttacatc tacaaaacac tacttgagac aatcatcgca
     2581 atattagtta aatcgcggtt tttgattagt ttaattcatg tgaatagtta agccagtcgc
     2641 cgcgttccct cttacactat gcgctgttat tagttcgtta ctggaagtcc agtcaccttg
     2701 tcaggagtat tatcgtgatt agtctattcg acatgtttaa ggtggggatt ggtccctcat
     2761 cttcccatac cgtagggcct atgaaggcgg gtaaacagtt cgtcgatgat ctggtcgaaa
     2821 aaggcttact ggatagcgtt actcgcgttg ccgtggacgt ttatggttca ctgtcgctga
     2881 cgggtaaagg ccaccacacc gatatcgcca ttattatggg tcttgcaggt aacgaacctg
     2941 ccaccgtgga tatcgacagt attcccggtt ttattcgcga cgtagaagag cgcgaacgtc
     3001 tgctgctggc acagggacgg catgaagtgg atttcccgcg cgacaacggg atgcgttttc
     3061 ataacggcaa cctgccgctg catgaaaacg gtatgcaaat ccacgcctat aacggcgatg
     3121 aagtcgtcta cagcaaaact tattattcca tcggcggcgg ttttatcgtc gatgaagaac
     3181 actttggtca ggatactgcc aacgaagtga gcgtgccgta tccgttcaaa tctgccaccg
     3241 aactgctcgc gtactgtaat gaaaccggct attcgctgtc tggtctcgct atgcagaacg
     3301 aactggcgct gcacagcaag aaagagatcg acgagtattt cgcgcatgtc tggcaaacca
     3361 tgcaggcatg tatcgatcgc gggatgaaca ccgaaggtgt actgccaggc ccgctgcgcg
     3421 tgccacgtcg tgcgtctgcc ctgcgccgga tgctggtttc cagcgataaa ctgtctaacg
     3481 atccgatgaa tgtcattgac tgggtaaaca tgtttgcgct ggcagttaac gaagaaaacg
     3541 ccgccggtgg tcgtgtggta actgcgccaa ccaacggtgc ctgcggtatc gttccggcag
     3601 tgctggctta ctatgaccac tttattgaat cggtcagccc ggacatctat acccgttact
     3661 ttatggcagc gggcgcgatt ggtgcattgt ataaaatgaa cgcctctatt tccggtgcgg
     3721 aagttggttg ccagggcgaa gtgggtgttg cctgttcaat ggctgctgcg ggtcttgcag
     3781 aactgctggg cggtagcccg gaacaggttt gcgtggcggc ggaaattggc atggaacaca
     3841 accttggttt aacctgcgac ccggttgcag ggcaggttca ggtgccgtgc attgagcgta
     3901 atgccattgc ctctgtgaag gcgattaacg ccgcacggat ggctctgcgc cgcaccagtg
     3961 caccgcgcgt ctcgctggat aaggtcatcg aaacgatgta cgaaaccggt aaggacatga
     4021 acgccaaata ccgcgaaacc tcacgcggtg gtctggcaat caaagtccag tgtgactaat
     4081 acttcttact cgcccatctg caacggatgg gcgaatttat ccccgctttc tcgtctgctg
     4141 taatattccc cactacactt ccactgttgc gtcaggcgtt tgtcgccata cgcttacaga
     4201 gtggcccgca tgcaaaaagc acaacggatc attaaaacct atcgccgtaa tcgaatgatt
     4261 gtttgtacga tttgcgcact cgttacgctc gcttcgaccc tgagcgtgcg atttatttca
     4321 cagcgtaact taaatcaaca acgggtagta caattcgcca atcacgctgt agaggaatta
     4381 gataaagtac tgcttcccct acaggcaggt agcgaagtct tgcttccgct gattggtctg
     4441 ccctgctctg tcgcccattt gccattacgt aaacaggcgg caaaactcca aactgtgcga
     4501 tccattggcc tggtgcaaga cggcacactt tattgctcca gcatttttgg ttatcgcaat
     4561 gtgcccgtcg tggacattct ggctgaactt cctgcaccgc aaccactttt acgcctgacg
     4621 accgaccgtg tcctgattaa aggcagtccg gttttgattc aatggacgcc tgcagcgagc
     4681 agtagcaatg ctggggtcat ggagatgatt aacatcgact tactgacggc aatgctgctt
     4741 gagccacaac tgccgcaaat cagtagcgcc agcctgacgg tggacaaacg gcatttgctc
     4801 tatggtaatg ggctggtaga ttcccttcca caacctgaag acaatgaaaa ctatcaggtt
     4861 tcttcgcaac gcttttcttt taccattaac gttaatggtc cgggggctcc ggcgctggca
     4921 tggcactatc ttccaacaca attaccgctg gcggtactgc taagtttact ggtgggctac
     4981 atcgcctggc tggcgaccgc ttaccgaatg agcttttccc gcgaaatcaa tctgggcctg
     5041 gcgcaacatg agttcgaatt gttctgtcag cctttgctta atgcgcgcag ccagcaatgt
     5101 attggtgtag agattttgct gcgctggaac aatccgcgtc agggctggat ttcaccggat
     5161 gtgtttattc ctatcgcgga agaacatcat ttaattgtgc cactgacccg ctatgtgatg
     5221 gcagaaacca ttcgtcagcg ccatgttttc ccgatgagta gtcagtttca tgttggcatt
     5281 aacgtcgcac ccagccattt tcgccgtggt gtgctgataa aagatctcaa tcagtactgg
     5341 tttagcgctc acccgattca gcaactgatc ctcgaaatca ccgaacgcga tgccttactg
     5401 gatgttgatt atcggattgc ccgcgagcta catcgtaaaa acgtcaaact ggcgattgat
     5461 gacttcggca ccggcaacag ttcattttcc tggcttgaaa cattacgtcc tgacgtgctg
     5521 aaaattgata agtcatttac cgcagctata ggttctgacg cggttaactc gacggtgacc
     5581 gatatcatca tcgcgctggg gcaaagactg aatattgaac tggtggcgga gggcgtggaa
     5641 acgcaagaac aggcgaagta tttgcgccgt catggcgtgc atattttgca agggtatttg
     5701 tacgcacagc cgatgccgct acgtgatttt cccaaatggc tggcgggcag ccaaccgccg
     5761 cccgcccggc ataatggaca tatcacgccc attatgccgt tacgttaacg cttactcatc
     5821 ttcatcgtgc gccggttgct ctttaacaat gcgaaccaga tcaacacgat aatcattggc
     5881 ttcaatgatg gtgatatgca gtggccctac atcaatcaca tcgcccacac ggggaatgtg
     5941 accatttgcc gagatcacga ggcccgcgac cgtcgcgata tcatcgtcat cggcaaggtg
     6001 ctcaacatca agcgcctgct gcaaggcatg caaatctgta ccgcctttta ccagccagcc
     6061 gtcaccatca gtaatgattt ccggcgtttc gtcagcgtcc gggaattcac ccgcaatggc
     6121 ttccagcaca tccagcggcg tgaccaagcc ttgtaccaca ccaaactcgt tggtcacgat
     6181 aacaaagctc ccgcgagcac gacgcagcac gcccagcagg ttgatcggat cgagggtttc
     6241 agggacgata atcgccggag acgccgaagc aatcgccgcc acatcaacgc cctcttccag
     6301 cgccaccagc agttctttag cacgtacgat tccgatgatt tcatccagtt caccgcgaca
     6361 taccgggaac aggctgtgcg gtgaggagag cagctgctcg cggatttcat cgaccccgag
     6421 attagcgtca acccagctta tttcaccgcg cggcgtcatg atcccgcgca gagaacgcga
     6481 cgccagcgtc agtacgccgt taatcatgta acgttcttct tcggcaaatg caccttccgg
     6541 gatcggcatc ggcatcgggt tatcggcatc gtgctgaaca ttggcctgac gtttcccgcc
     6601 catcaaacgc aggatggcat cggcagtacg cgctcgcagc ggcaaagtcg actggtgacg
     6661 aataaagttg cgacgcgcaa tctggttaaa cacttcgatg atgatcgaga agccaatcgc
     6721 ggcatacagg taacctttcg gaatgtggaa accgaaacct tctgccacca gactcagacc
     6781 aatcattaac aggaagctca gacagagcac caccaccgtg gggtgctggt taacgaatcg
     6841 cgtcagcggt ttggatgcca gcaacataac cgccatcgca atcactaccg ccgccatcat
     6901 caccggcaga tggttaacca tccctactgc agtaattacc gcatccaacg agaagacggc
     6961 gtcaaggatg acaatctgtg tgacgaccac ccagaaactg gcgtagcctt taccgtggcc
     7021 ggaatcatga tcgcggtttt ccagccgttc atgcagttcg gttgttgctt tgaacagcaa
     7081 gaatatcccc ccgaacaaca taatcaggtc gcgtccggag aaggagaaat ccatgacggt
     7141 aaatagcggt ttggtcagcg tgaccatcca tgaaatcagc gacagcagcc ccagacgcat
     7201 aatcagcgcc agtgataacc ccagcaaacg cgctttatcg cgttgttttg gcggcagttt
     7261 gtcagcaaga atggcgatga agaccaggtt atcgataccc agcacaattt cgagaacaac
     7321 aagcgtgagt agccccgccc aaattgaggg gtccattaag aattccatga caagctcctg
     7381 cttaaggaat agctattcga cgccagaaat aatgcaggcg taacgacaaa atgcaaacga
     7441 aaggtgcggc atagagtgcc agaaaggcag gcgttaaaag gcctgatgct gaaatgacgt
     7501 cggtgacgat ccatactgcg ggctactgcc ctatactcca tgattgttaa acgggagtta
     7561 aacatatcag agacgcctct gatttggcaa agatttacct tcctttgcaa acgaatgtga
     7621 caaggatatt ttacctttcg aaatttctgc taatcgaaag ttaaattacg gatcttcatc
     7681 acataaaata attttttcga tatctaaaat aaatcgcgaa acgcaggggt ttttggttgt
     7741 agcccttatc tgaatcgatt cgattgtgga cgacgattca aaaatacatc tggcacgttg
     7801 aggtgttaac gataataaag gaggtagcaa gtgaccattg ctattgttat aggcacacat
     7861 ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc tgttaggcga gcaggaaaac
     7921 gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga aaagtacaac
     7981 gctcagttgg caaaactcga caccactaaa ggcgtgctgt ttctcgttga tacatgggga
     8041 ggcagcccgt tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta tgaagtcatt
     8101 gcaggcgtta acattccaat gctcgtggaa acgttaatgg ctcgtgatga tgacccaagc
     8161 tttgatgaac tggttgcgct ggcagtagaa acaggccgtg aaggcgtgaa agcactgaaa
     8221 gccaaaccgg ttgaaaaagc cgcgccagca cccgctgccg cagcacccaa agcggctcca
     8281 actccggcaa aaccaatggg gccaaacgac tacatggtta ttggccttgc gcgtatcgac
     8341 gaccgtctga ttcacggtca ggtcgccacc cgctggacca aagaaaccaa tgtctcccgt
     8401 attattgttg ttagtgatga agtggctgcg gatactgttc gtaagacact gctcacccag
     8461 gttgcacctc cgggcgtaac agcacacgta gttgatgttg ccaaaatgat tcgcgtctac
     8521 aacaacccga aatatgctgg cgaacgtgta atgctgttat ttaccaaccc aacagatgta
     8581 gagcgtctcg ttgaaggcgg cgtgaaaatc acctctgtta acgtcggtgg tatggcattc
     8641 cgtcagggta aaacccaagt gaataacgcg gtttcggttg atgaaaaaga tatcgaggcg
     8701 ttcaaaaaac tgaatgcacg cggtattgag ctggaagtcc gtaaggtttc caccgatccg
     8761 aaactgaaaa tgatggatct gatcagcaaa atcgataagt aacgtattgt gttgattatc
     8821 agtcagtttt cacactttag tcatacgtaa acaggagaag tacaatggag attaccactc
     8881 ttcaaattgt gctggtattt atcgtagcct gtatcgcagg tatgggatca atcctcgatg
     8941 aatttcagtt tcaccgtccg ctaatcgcgt gtaccctggt gggtatcgtt cttggggata
     9001 tgaaaaccgg tattattatc ggtggtacgc tggaaatgat cgcgctgggc tggatgaaca
     9061 tcggtgctgc agttgcgcct gacgccgctc tggcatctat catttctacc attctggtta
     9121 tcgcaggtca tcagagcatt ggtgcaggta ttgcactggc aatccctctg gccgctgcgg
     9181 gccaggtact gaccatcatc gttcgtacta ttaccgttgc tttccagcac gctgcggata
     9241 aggctgctga taacggcaac ctgacagcga tttcctggat ccacgtttct tctctgttcc
     9301 tgcaagcaat gcgtgtggct attccggccg tcatcgttgc gctgtctgta ggtaccagcg
     9361 aagtacagaa catgctgaat gcgattccgg aagtggtgac caatggtctg aatatcgccg
     9421 gtggcatgat cgtggtggtt ggttatgcga tggttatcaa catgatgcgt gctggctacc
     9481 tgatgccgtt cttctacctc ggcttcgtaa ccgcagcatt caccaacttt aacctggttg
     9541 ctctgggtgt gattggtact gttatggcag tgctctacat ccaacttagc ccgaaataca
     9601 accgcgtagc cggtgcgcct gctcaggcag ctggtaacaa cgatctcgat aacgaactgg
     9661 actaacaggt gagcgaaatg gttgatacaa ctcaaactac caccgagaaa aaactcactc
     9721 aaagtgatat tcgtggcgtc ttcctgcgtt ctaacctctt ccagggttca tggaacttcg
     9781 aacgtatgca ggcactgggt ttctgcttct ctatggtacc ggcaattcgt cgcctctacc
     9841 ctgagaacaa cgaagctcgt aaacaagcta ttcgccgtca cctggagttc tttaacaccc
     9901 agccgttcgt ggctgcgccg attctcggcg taaccctggc gctggaagaa cagcgtgcta
     9961 atggcgcaga gatcgacgac ggtgctatca acggtatcaa agtcggtttg atggggccgc
    10021 tggctggtgt aggcgacccg atcttctggg gaaccgtacg tccggtattt gcagcactgg
    10081 gtgccggtat cgcgatgagc ggcagcctgt taggtccgct gctgttcttc atcctgttta
    10141 acctggtgcg tctggcaacc cgttactacg gcgtagcgta tggttactcc aaaggtatcg
    10201 atatcgttaa agatatgggt ggtggcttcc tgcaaaaact gacggaaggg gcgtctatcc
    10261 tcggcctgtt tgtcatgggg gcattggtta acaagtggac acatgtcaac atcccgctgg
    10321 ttgtctctcg cattactgac cagacgggca aagaacacgt tactactgtc cagactattc
    10381 tggaccagtt aatgccaggc ctggtaccac tgctgctgac ctttgcttgt atgtggctac
    10441 tgcgcaaaaa agttaacccg ctgtggatca tcgttggctt cttcgtcatc ggtatcgctg
    10501 gttacgcttg cggcctgctg ggactgtaag actgttgtac actaccgggg ccttttggcc
    10561 ccgttttttt atctggagga ttaatgacaa tcacggacct ggtactgatt cttttcatcg
    10621 ccgcactcct ggccttcgcg atctacgatc agttcatcat gccccgccgt aacggcccca
    10681 ccctgctggc aattcctttg ctccggcgtg gtcgcatcga tagcgttatc ttcgtcggat
    10741 tgattgtcat tcttatctat aacaacgtca cgaatcatgg tgcgttaata acgacatggt
    10801 tattaagcgc actggctctg atgggttttt atatattctg gatccgcgtt ccgaagatca
    10861 tctttaaaca aaaaggtttt ttcttcgcca atgtctggat tgaatatagc cgaatcaaag
    10921 cgatgaactt gtcggaagat ggcgtgttgg tgatgcaatt agaacagcgt cggctgttaa
    10981 tccgcgttcg aaatatcgac gatctggaaa gaatttataa acttctcgtt tcaactcaat
    11041 gagttattaa tttagccaaa gctatgttta gtgtattttt aataatcaga catagcttag
    11101 gctatattac ctcttccctt atttgttatt tattataacg tttcattgat atataaatct
    11161 aaatgaaaat cgttatcaac aaagcaataa aataatatat tccaacagtt gttttatatt
    11221 ctcaaaatat gttaaggttg cgccctcatt tggggagtag ccaatttcca gattccggaa
    11281 atgtacgtgt caacatactc gttgcaaaac gtggcacgta cggactgaag actttcagtc
    11341 aggcgagacc atatgcacat caatcgctat gcctgcatga acgcgaaaac ccatcgcggc
    11401 ttgttgttca tgtatgcatg tttgctgggg gcgatgatgt gttttatgga taccccggtc
    11461 aggacgttgt catgaatatc actgctactg ttcttcttgc gtttggtatg tcgatggatg
    11521 catttgctgc atcaatcggt aaaggtgcca ccctccataa accgaaattt tctgaagcat
    11581 tgcgaaccgg ccttattttt ggtgccgtcg aaaccctgac gccgctgatc ggctggggaa
    11641 tgggcatgtt agccagccgg tttgtccttg aatggaatca ctggattgcg tttgtgctgc
    11701 tgatattcct cggcgggcga atgattattg agggttttcg tggcgcagat gatgaagatg
    11761 aagagccgcg ccgtcggcac ggtttctggc tactggtaac caccgcgatt gccaccagcc
    11821 tggatgccat ggctgtgggt gttggtcttg ctttccttca ggtcaacatt atcgcgaccg
    11881 cattggccat tggttgtgca accttgatta tgtcgacatt agggatgatg gttggtcgct
    11941 ttatcggctc aattattggg aaaaaagcgg aaattctcgg cgggctggtg ctgatcggca
    12001 tcggcgtcca gatcctctgg acgcacttcc acggttaata agaacgctgc cagaggtgaa
    12061 tattaaagtc cgtctggcag tctaacactt cttttgctgc cagtatttgc cagacttctg
    12121 gcttcgcacg ccaggcaaac ggcgtcatct gcaataatgc gacggcttca tcaccgcgaa
    12181 gacgcatcgg ataacacaac tcatcactct tctgtaatgt aaaaccttcc agttgttctg
    12241 catgaggtgc atgaagatgt acttcattgt aaatcagccc cttcagctcc atcaaatgtc
    12301 gcggtcccgg cgtggcagta atgacccagc cgccgggctt cactactcgt gctaattctt
    12361 ctgctttaca cggcgcgtaa atacgtatta tggcgtccat actggtatcg gaaaacggca
    12421 aacggtggct ggaagcgaca caaagagtga cctgcggata gcgtttcgcc gccgctttta
    12481 tcgctacctt cgaaacatcc agaccaaacg tggtgatttc gggcaacgca tcggcaaatg
    12541 cgtgtgtgta atacccttca ccacagccaa tatccagcac cgccgtggcc ttttcattaa
    12601 gccgttcccg cagttgggcg acaattgcat cacgcagcgg ctgataatgt ccggcatcta
    12661 agaatgcgcg gcgtgcttgc atcatttccg cgctgtcgcc cggatcacga gaccgtttat
    12721 gctgaacggg cagcagattg acataccctt ctttcgccat atcaaactga tgtcgttggg
    12781 gacagatata gctgtttttt tcacgcgtaa gaggctgatg gcaaagtgga caggaaaacg
    12841 acatgacaac tccggcagga tactaaaggc cgcaagtgta acgcgaatca cgcggcgggc
    12901 aaatatccga acgccaggag cgaagataaa gcatagtaaa aagcctcgca ttcgacgagg
    12961 ctttatagcg agattgaagc gtattcacac ttcagatcag tggattcgat cagatagctg
    13021 ttacgttaac agctgccgga cctttctggc cgtcctgaat ttcgaactca acgttctgac
    13081 cttcagccag agttttgaag ccattaccct ggatagcgga gaagtgtacg aacacgtctt
    13141 tgctgccatc agcaggagta atgaagccaa aacctttaga ctcgttgaac cacttaacct
    13201 gacctttaat ctttgccatt tgaaaaattc cttagattgt tttcttcgcc cgcaggcata
    13261 acatagataa aactgacaca ttactgcatg aggcaccaat ataaggctcg gcagagaagc
    13321 ggtattcaac gtcaacgtgt ttactcagga cttctttact gaaaatgcca cacataaaca
    13381 gaactgtacc tcgtttaacc cgaaatctgt tatcacatac aacgttaatt atggcaagcc
    13441 atttttaaac atgtctcgat cagacgcaca aatctcgata cgtcttccac tttttttgca
    13501 cacttatgca acggaatacg cgccgatata tcattgtgct taaccttgtc agttccgcag
    13561 gtacttaaac tgacgtagtt tctaacatag ttcaataagt tcagttgttc caggtcagtg
    13621 ggagaagtta ttacatagtg cgtgcaatat cacattttct gctatgcaat gaataaaaag
    13681 ttatatcact ttttctcata aaacaggcag ttaacggctc tcaatcaccc caaagagaag
    13741 tcaatcccaa aggaattgga aatttaaaat aagaaaaatt gatgaataag caaaaaaatc
    13801 aagagagaaa cgtttcgcta ataaataata ccacgcttaa tcacaaatcc gatgtaaaca
    13861 tcctaataaa ttaatggggt aaatatcttt ttctgatatc ccgttacgca aataaataat
    13921 atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagttgcac catgacagtg
    13981 cgtataatca ggatgaattg agtaaccctt caacgacggg gttactcaag attgaggaag
    14041 gattatcgtt cggcgaccag gcgaataata tcttcgtctt caaccgattt ttgactctca
    14101 atagtctttt tatctgcttt ctctggttcg tttgcttcgc acaaacgacg taacagtgca
    14161 ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc gttagtccga
    14221 gaactggcac gattaataaa aaaccacaag atgagcccga taagaagaac caccaccgat
    14281 acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat ttcaccacct
    14341 ctgagtagag gcgctattct accactgctg gagaggaaga aaatctagtg ctgaaaaaat
    14401 gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat tgtacatcct
    14461 gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac accacgacaa
    14521 ccaaaacgac ccatcgaaac tttttcactc caatctccgt ttcacctacc ttatgtcatt
    14581 aaactaccat gcgttagtta aacagcgtct aactatgtcg atattcgtgc tttttcggaa
    14641 tgagtcgctt gcttcagcgc atattgccgc tacgattaag cgaacataaa aaagagaaga
    14701 ggttgtaatg cgattcatca ttcgcacagt tatgctgata gcactggtat ggataggatt
    14761 attactcagc ggatatggcg ttttgattgg aagcaaagag aatgccgcag aattaggttt
    14821 gcaatgtacc tatctgactg ccagaggcac cagcacggta caatacttgc acactaaaag
    14881 tggttttttg gggataacag attgcccgct gttacgcaaa agcaatatcg tggtcgataa
    14941 tggttaagtg taataaataa ccagattct
//
LOCUS       DBFIFS010000109        14956 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.109, whole genome shotgun sequence.
ACCESSION   DBFIFS010000109 DBFIFS010000000
VERSION     DBFIFS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.109"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            139..381
                     /gene="yjbE"
                     /locus_tag="N7A07_002888"
     CDS             139..381
                     /gene="yjbE"
                     /locus_tag="N7A07_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU2477517.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            495..1133
                     /gene="yjbF"
                     /locus_tag="N7A07_002889"
     CDS             495..1133
                     /gene="yjbF"
                     /locus_tag="N7A07_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU2477518.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1130..1867
                     /gene="yjbG"
                     /locus_tag="N7A07_002890"
     CDS             1130..1867
                     /gene="yjbG"
                     /locus_tag="N7A07_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU2477519.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1867..3963
                     /gene="yjbH"
                     /locus_tag="N7A07_002891"
     CDS             1867..3963
                     /gene="yjbH"
                     /locus_tag="N7A07_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU2477520.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4010..4288)
                     /locus_tag="N7A07_002892"
     CDS             complement(4010..4288)
                     /locus_tag="N7A07_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477521.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4558..4968
                     /gene="psiE"
                     /locus_tag="N7A07_002893"
     CDS             4558..4968
                     /gene="psiE"
                     /locus_tag="N7A07_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU2477522.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="N7A07_002894"
     CDS             complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="N7A07_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU2477523.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6859..7749)
                     /gene="malG"
                     /locus_tag="N7A07_002895"
     CDS             complement(6859..7749)
                     /gene="malG"
                     /locus_tag="N7A07_002895"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU2477524.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7764..9308)
                     /gene="malF"
                     /locus_tag="N7A07_002896"
     CDS             complement(7764..9308)
                     /gene="malF"
                     /locus_tag="N7A07_002896"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU2477525.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9462..10652)
                     /gene="malE"
                     /locus_tag="N7A07_002897"
     CDS             complement(9462..10652)
                     /gene="malE"
                     /locus_tag="N7A07_002897"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU2477526.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11017..12132
                     /gene="malK"
                     /locus_tag="N7A07_002898"
     CDS             11017..12132
                     /gene="malK"
                     /locus_tag="N7A07_002898"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU2477527.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12204..13544
                     /gene="lamB"
                     /locus_tag="N7A07_002899"
     CDS             12204..13544
                     /gene="lamB"
                     /locus_tag="N7A07_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU2477528.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13695..14615
                     /gene="malM"
                     /locus_tag="N7A07_002900"
     CDS             13695..14615
                     /gene="malM"
                     /locus_tag="N7A07_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU2477529.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14844..>14956
                     /locus_tag="N7A07_002901"
     CDS             14844..>14956
                     /locus_tag="N7A07_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477530.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGGP"
BASE COUNT         3689 a         3842 c         3943 g         3482 t
ORIGIN      
        1 ttgatattca ttccaattat cctaaaaccc catctctaat tccccgcgcc gtaattcgca
       61 tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt
      121 caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt
      181 gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca
      241 acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg
      301 ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc
      361 acgaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc
      421 tgtggctaga agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc
      481 tctggagaag agtcgtgaag cgacctgcat tcattcttat ctgcctgcta ttacaggcct
      541 gttcagccac gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag
      601 gcgtacagct gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc
      661 ttaatggtgg gccgcagttg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat
      721 gggtcactca ggatcaggcc actctcgtca cacaacatgg tcgtctggtg aagactttgc
      781 ttggcggcga caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg
      841 cacaaatcat tgatggcgca acctggatgc gcacgatggg ctggaccgag taccagcagg
      901 tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccggtacc gttaaggtcg
      961 gcagcgatga aaccgcggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct
     1021 ggcataaccg ctattggatc gacagcggag ggcaaattcg ccagtcggaa cagtatctcg
     1081 gcgcggatta tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca
     1141 aactattgtc gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt
     1201 caaggtgttc agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat
     1261 cgatctggta ggccaaccgc ggctggcaaa cagctggtgg cctggtgcgg tgattagcga
     1321 agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga
     1381 acagggcgca gattccagcg ccgacgatgc cgctgcgatt aacgccttac gccagcaaat
     1441 tcaggcgttg aaggtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt
     1501 tgctgaacgc gctaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccacc
     1561 gtccacggtc acgttgttcg ggcttatcag ccgtcctggc aatcagccat tcactcccgg
     1621 tcgcgacgtg gcgagctatc tctccgggca aagtctgctt agcggtgcgg atcgtagcta
     1681 cgcgtgggtg gtttacccgg acggacgcac acaaaaagcg ccggtggctt actggaacaa
     1741 gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg
     1801 gagtgagatg cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc
     1861 tcaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc
     1921 tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga
     1981 ttattacaaa cgcccacggc tcgcatggcg cgggaagggg agttgagtct gaactatcgc
     2041 gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca
     2101 acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc
     2161 gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac
     2221 tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg
     2281 gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgcttgg cctgggctgg
     2341 ggatatttgg gcacaagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat
     2401 tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac
     2461 ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg
     2521 aaactggagt atgaaggcaa taattatcag caggattttg ccggtaagct ggagcaaaaa
     2581 agtaagttta acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc
     2641 tatgaacgtg ccaacacttt tatgtttggc gtcacgctgc gcaccaactt taacgatctg
     2701 cgcccgtctt acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt
     2761 ttgcagcatt cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc
     2821 gatccgcaga tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat
     2881 cgtgattcgc gcgaagggat cgttcgtgct aatcggatcg tgatgaatga tctgccggat
     2941 gggatcaaga cgatccgcgt tacggaaaat cgccttaaca tgccgcaggt gacgacggaa
     3001 acggatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag
     3061 ctggcgcaaa aacgcgtcga gccagtggtt ccgaagtcca ccgagcaggg ctggtatatc
     3121 gacaaatcgc gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg
     3181 gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac
     3241 catctgctga ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac
     3301 tacaccaatc cgccgcagga ctcgcactta ccgcgcgtgc gtacccatgt gcgcgagtat
     3361 gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac
     3421 ggcttctacg gtcaggtcta cggaggttat ctcgaaacca tgtttggcgg tgcgggggca
     3481 gaagtgttgt atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt
     3541 aaacagcgcg actggcgtag tgcaaaggat atgatgaaat tcaccgacta cagcgtgaaa
     3601 accggacatc tgaccgccta ctggacgcca tctttcgctc aggatgtgtt agttaaagcc
     3661 agcgtcgggc agtatctggc aggggataaa ggcggcacgc tggagatcgc taaacgcttt
     3721 gacagcggcg tggtggtggg cggctatgcc acgatcacca acgtttcgaa agaggagtac
     3781 ggcgaagggg acttcaccaa aggcgtgtat gtctctgtac cgttggatct cttctcgtct
     3841 ggcccgacac gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag
     3901 caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc
     3961 taagtcatgg gaaaggtgcc agttttcgca atcactggca cctttattct taatggcctg
     4021 ttgagccatc aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac
     4081 attctgtctt cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca
     4141 tcattgggct ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac
     4201 cagcataagc aggaagagca gagactgata acatcaaagc agcaaaataa ggcttcattt
     4261 ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg
     4321 cattgctggt ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg
     4381 gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc
     4441 ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga
     4501 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     4561 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     4621 cttggcctgc tgtgcctcgg ccttattttg gttgtcttcc tcggcaaaga aacggtgcat
     4681 ctggctgatg tgctgttcgc gccagaacaa gccagcaaat atgagctggt agaaggactg
     4741 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     4801 tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc
     4861 atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     4921 gtggtcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg
     4981 ccgaactggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     5041 tcgtttccgg ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga
     5101 caaatttcca cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga
     5161 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     5221 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttacctcgaa
     5281 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     5341 aggcggcaac atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa
     5401 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     5461 gcttacgacc aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa
     5521 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     5581 cttccggcgc gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca
     5641 taacgccgat gacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     5701 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     5761 ttttgcgcag gataccttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac
     5821 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     5881 acatataacg ccagccgtca gtattcagcc agctggcatc accggaacgc gcaataaaat
     5941 agtttacgca gtaaactaaa agctgcccga aaataatcgc aaactggtta aatgaaacca
     6001 gtttcccgcg aatatgagca ggagcaagtt ccgcaatata cattggtgaa agcattgagg
     6061 ctaaaccaac gccaataccg ccaataatac gataaataac aaattccggg acataacctg
     6121 ccagataaac aggcactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
     6181 cagaacctac accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacgac
     6241 cgaagcggtt actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc
     6301 tggccacgca aaaccctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
     6361 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
     6421 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
     6481 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
     6541 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
     6601 gatcttaatt gtgataatta tccaaaattg ataaaaacaa tactattgcc gtgactcaga
     6661 gcacgaaaga gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg
     6721 atggaaatat taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt
     6781 ggagcgccga ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     6841 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     6901 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     6961 aaagtcaccc cacaggtagt tttgcggatt gaggtattgc tgcatcccca cggccagggt
     7021 gtagctgttt acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat
     7081 aaacgacagg atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     7141 gaaggcctgc cacggtgtcg caccatccag cgcagccgct tcttccagcg aactgtcgat
     7201 ggtttcgaaa tagcctttga tggtccagac gtgcagcgca atcccaccca gatacgcgaa
     7261 aattacgccg ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa
     7321 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     7381 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     7441 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     7501 cagcagcacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc
     7561 taatgccagt ttccagtgat cccaggagat ttgctccggg atcaggctgc cggtcgcaaa
     7621 gttcccctgg cgcagtgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat
     7681 aaaaagtagc agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac
     7741 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     7801 atcgccagcg cacccaccag caggaagatc agcgtggcaa ttgctgctgc cagaccgaag
     7861 tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt agttaacaag caggtcggta
     7921 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc
     7981 agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta
     8041 atcagcagcg gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct
     8101 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     8161 tacggatagc ccagccaggt attgacgata atcagcatcg ttcgggcggt ggtcggatcg
     8221 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ctcaccgaag
     8281 ctctggttaa acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     8341 agaatcagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac
     8401 gccagaacca tgccgactgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     8461 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgagtaaag
     8521 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg
     8581 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     8641 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac
     8701 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     8761 agcgcctgac gattctgggt aatcacgcgt agatttgcgc gttcgccttc gggctgggcg
     8821 gtcgtttctt tcagttgcag tttttgctct ctgccaaatt taaaagcgtc ggagaggtaa
     8881 tttttgccgg tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga
     8941 ccaaagttat aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgtgcacgt
     9001 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     9061 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     9121 taggctttac gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg
     9181 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     9241 agcagaccta acactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     9301 acatccatgg ggttcttcct cattccagga cggataaggc tttcatgcct tatccgacaa
     9361 caactgcctg atgcgacgct gacgcgtctt atcaggccta catacgtttc ggttttgtag
     9421 gccggacaag gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc
     9481 tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca
     9541 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc
     9601 tgggcgtttt ccatggtggc tgcaatacgt ggatctttcg ccaactcttc ctcgtaagac
     9661 ttcagcgcta cggcacccag cggtttgtct ttattaaccg cttccagacc ttcgtcagtc
     9721 agcagatagt tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata
     9781 ccggcgctca gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc
     9841 gttacaccat aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgata
     9901 gtcatcgctg tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca
     9961 ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca gacccgcttt cgcgccagcg
    10021 ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc
    10081 ccgtcagcag caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg
    10141 ctcttacctt tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc
    10201 gggttcggta gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa
    10261 gcaatcagct tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg
    10321 aacgctttgt ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg
    10381 tcgtgcgccc agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc
    10441 tcttccagtt tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc
    10501 ttaccgactt cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta
    10561 ccttcttcga ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat
    10621 gcgaggatgc gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct
    10681 cgtgaaaaca cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt
    10741 gcccctacgc cccaccgttg ctttgtgtga tctctgttac agaattggca gtaatgtgga
    10801 gatgcgcaca taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac
    10861 ctcggtttag ttcacagaag ccgcgttctc atcctcccgc ctcctccccc ataaaaaagc
    10921 tagggggtgg aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt
    10981 gctgtcgatg acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat
    11041 gtaacgaaag cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa
    11101 ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg
    11161 attgccgggc ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat
    11221 gacactccgc cagcagaacg tggcgttggt atggtgtttc agtcctacgc gctctatccc
    11281 cacctgtcag tagcagaaaa catgtcattt ggcctgaaac tggcaggcgc aaaaaaagag
    11341 gtgattaacc aacgcgtcaa ccaggtggcg gaagtgctac aactggcgca tttgctggat
    11401 cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg
    11461 gtggccgagc caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg
    11521 cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt
    11581 tacgtcaccc acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac
    11641 gccggtcgcg tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt
    11701 tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc
    11761 accgcaatcg atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg
    11821 ccagttgaaa gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa
    11881 catctactgc cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag
    11941 caactcggca acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg
    12001 taccgccaga acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg
    12061 ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag
    12121 ccgggcgttt aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga
    12181 aaagcaatga ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg
    12241 ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac
    12301 gttccggtat tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg
    12361 ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc
    12421 aggaagtgtg gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg
    12481 tcgcgcaaca gaatgactgg gaagctaccg acccggcctt ccgtgaagca aacgtgcagg
    12541 gtaaaaacct gatcgaatgg ctgccaggct ccaccatctg ggcaggtaag cgcttctacc
    12601 aacgtcatga cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg
    12661 gtctggaaaa catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg
    12721 aagcaggtgg ttcttcctct ttcgccagca acaatattta tgactatacc aacgaaaccg
    12781 cgaacgacgt tttcgatgtg cgtttagcgc agatggaagt caacccgggc ggcacattag
    12841 aactgggtgt cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg
    12901 catcgaaaga cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta
    12961 acaagtttgt tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc
    13021 agggttctgg cgttgcattt gataacgaaa aatttgccta caatatcaac aacaacggtc
    13081 acatgctgcg tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt
    13141 acgtgggtat gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg
    13201 tcggtattcg cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct
    13261 acgacaacgt cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg
    13321 cacaacaatg gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa
    13381 cctacgccaa gtgggatgag aaatggggtt acgactacaa cggcgatagc aaggttaacc
    13441 cgaactacgg caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca
    13501 gcgacgagtg gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc
    13561 ggataaggcg tttacgccgc atccggcata aaacaggttg tcattatctg aaaggggcga
    13621 aagcccctct gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt
    13681 ttgaggtgaa aacaatgaaa atgaataaaa gtctcatcgc cctctgttta tcagcagggt
    13741 tactggcaag cgcgcctgga atcagccttg ccgatgttaa ctacgtaccg caaaacacca
    13801 gcgacgcgcc agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc
    13861 aatctaaaac ccagaccacc caactggcga ccggcggcca acaactgaac gttcccggca
    13921 tcagtggtcc ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctgacgc
    13981 tgaccagcga agtgaacaaa caaaccagcg tttttgcgcc gaacgtgctg attcttgatc
    14041 agaacatgac cccatcagcc ttcttcccca gcagttattt cacctaccag gaaccaggcg
    14101 tgatgagtgc agatcggctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa
    14161 aactttatgt tctggtcttt accacggaaa aagatctcca gcagacgacc caactgctcg
    14221 acccggctaa agcctatgcc aaaggcgttg gtaactcgat cccggatatt cccgatccgg
    14281 ttgctcgtca taccaccgat ggcttactga aactgaaagt gaaaacgaac tccagctcca
    14341 gcgtgttggt cgggccttta tttggttctt ccgctccagc tccggttacg gtaggcaaca
    14401 cggcggcacc agctgtggct gcacccgctc cggtgccggt gaagaaaagc gagccgatgc
    14461 tcaacgacac ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg
    14521 ttgataaggc gttaaaactg cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt
    14581 ccacctttat cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc
    14641 aacaactggt gcgtctcctg gcgcaccttt ttttatgctt cattccaggg atatgagcga
    14701 ttttttatag taactcactt cttcttcact aagaatatcc attatctcaa tgcattatca
    14761 gagattcttt tcctttcgcc ggtagtgtct ggacgctcag gctacttttt ccagttattt
    14821 catttctgta atgcagaggt tttatgttag gtcatatctc aaagtttgac ggcaataact
    14881 ctttgataaa acatggtgtg gtgcaaggaa ataatatagt agattttgat ttactacgta
    14941 attttaatgg ggggcc
//
LOCUS       DBFIFS010000110        14913 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.110, whole genome shotgun sequence.
ACCESSION   DBFIFS010000110 DBFIFS010000000
VERSION     DBFIFS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.110"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..100
                     /locus_tag="N7A07_002902"
     CDS             <1..100
                     /locus_tag="N7A07_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477531.1"
                     /translation="LEYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            116..484
                     /gene="acpS"
                     /locus_tag="N7A07_002903"
     CDS             116..484
                     /gene="acpS"
                     /locus_tag="N7A07_002903"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU2477532.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            498..1259
                     /locus_tag="N7A07_002904"
     CDS             498..1259
                     /locus_tag="N7A07_002904"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU2477533.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            1263..1811
                     /locus_tag="N7A07_002905"
     CDS             1263..1811
                     /locus_tag="N7A07_002905"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HGU2477534.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            1833..2114
                     /locus_tag="N7A07_002906"
     CDS             1833..2114
                     /locus_tag="N7A07_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2477535.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            2150..3310
                     /locus_tag="N7A07_002907"
     CDS             2150..3310
                     /locus_tag="N7A07_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HGU2477536.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            3384..5942
                     /locus_tag="N7A07_002908"
     CDS             3384..5942
                     /locus_tag="N7A07_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HGU2477537.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            5947..7170
                     /locus_tag="N7A07_002909"
     CDS             5947..7170
                     /locus_tag="N7A07_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HGU2477538.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            7167..8120
                     /locus_tag="N7A07_002910"
     CDS             7167..8120
                     /locus_tag="N7A07_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2477539.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            8131..8838
                     /locus_tag="N7A07_002911"
     CDS             8131..8838
                     /locus_tag="N7A07_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2477540.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            8822..9478
                     /locus_tag="N7A07_002912"
     CDS             8822..9478
                     /locus_tag="N7A07_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477541.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            9479..10789
                     /locus_tag="N7A07_002913"
     CDS             9479..10789
                     /locus_tag="N7A07_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477542.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGGEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            10798..11586
                     /locus_tag="N7A07_002914"
     CDS             10798..11586
                     /locus_tag="N7A07_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HGU2477543.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            11583..12971
                     /locus_tag="N7A07_002915"
     CDS             11583..12971
                     /locus_tag="N7A07_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477544.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            12985..13926
                     /locus_tag="N7A07_002916"
     CDS             12985..13926
                     /locus_tag="N7A07_002916"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU2477545.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            13923..14909
                     /gene="ycdU"
                     /locus_tag="N7A07_002917"
     CDS             13923..14909
                     /gene="ycdU"
                     /locus_tag="N7A07_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU2477546.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWVHFNK"
BASE COUNT         3728 a         3402 c         3941 g         3842 t
ORIGIN      
        1 tcttgagtat aacttttccg tttctacccc attaaagtat ccggcgacat tagatattga
       61 tcgggtgaat gtaaatttta atttttcgta ccaaatgtaa aggataacga gactcatgag
      121 aatagggaca gatattgttg aaattgccag aataaccaaa gccattatta atggagagat
      181 ggctttttat gagcgtgttt atacttctca tgagcgctta ctcattaacc ctgcagaacc
      241 tgattatgaa cgggcctctg gattctgggc ggcgaaagaa gcgttagtta aagcgattgg
      301 ttgtggtttt cgtgatggcg tacgtttcca ggatattgaa ataaagcatg atgcagccaa
      361 agcaccttat ttcgttattt ccggaagact acaagaaatt tttatcgggc aggggttgca
      421 aaataactcc ctgagtattt cgcattgccg tacccacgcc attgcgactg tcatcgttta
      481 ttaactcaac atatgtgatg aatatgtaca aatataatga tttaaatgaa aaaaccgtac
      541 tggtgacagg agccagtggt gatattggac tgggtatttg cgaaaaatat ctggagcaga
      601 actgcgatgt ttatgcgctc tataaatcca acgacgtgca attaacggcg ctgaaagcaa
      661 gccatcctgc aggcgataaa ctgcatattg tgcagtgcga tcttgcctgc ccccagagcg
      721 tgagcgcatt gtgcgaacag atagagcgcc aagctggcaa gattgatgtg ctggtaaata
      781 acgcgggcat cgttaaagac agcctgtttg cctcgatgag ttatgaggat ttcacccagg
      841 ttatcgaaac caacatgttc agcattttcc gtctgacgaa agacgcgctg atgctgcttc
      901 gtgcggcaga aaatcccgcc attattaacg ttgcctctat tgcagcgctg atcccaagcg
      961 tcggtcaggc gaattacagc gcgtcaaaag gggcgatcct aggcttcacc cgcacgctgg
     1021 cagcagaaat ggcaccctgg ggcgtgcgcg tcaacgcggt cgcaccggga atgattgaat
     1081 cgaaaatggt gaaaaaagta tcccgcgcag tggtccgtgc ggtaaccagt accatcccgt
     1141 tacgccgtct gggtaaatgt gaagaagtgg cgaacaccat cgtcttctta agttcctctg
     1201 cctccagcta cattgtcggc cagaccatcg ttatcgatgg cggcctggtc atgcggtgat
     1261 ttatgtctaa atacacgatt ccttcgaaaa tcttcctcga aatgggcggc tggcgtcagc
     1321 cgctgctgat ggtcgataaa attgcagatt ataaatacgg tgaaaatggc tttgtcagtg
     1381 tcgtgaaaca cgtcacctat aacgaaccgt atctactggg gcatttcccg gaagatccga
     1441 ttatgcccgg ggtgattatt tcggaaatct tcggccaggc cagcgagtat ctctctttcc
     1501 tgaccgacat ttgcgatatc tggcgcgaac gttttgagga agagctgaag tcgttacgtg
     1561 atattcacgc acgcattcac cgccctgaaa tgctggagat tatccgtacc cggcgcagcc
     1621 aggtacgcgg cgtgctggcg gctcagaacc tgaaatttaa agatatcgcc tatccgggtg
     1681 attctattga tgttgtcagc aaattggctt tttcggatgc cagtggcttt aaacactatt
     1741 ccgtgaccgc gtacgtcggt aaaaagctga ttagtcaggg caccattatc aatttccgtg
     1801 aaaccaagta atccaataag agagtaaata ttatgtctac catgaatgac gctattgaac
     1861 agcgtaaaga agttctgatg accattaaaa ctgagatcat ccagcgactg aatattcaac
     1921 gcgatgaagc gcaaattgat gatgatactg cgctgtttgg taatggtttt aaactggact
     1981 ccgtcgatgc aacggaaatt gtcgttctgc tggataaagt atttgatatc aagatcagcg
     2041 aaagtgacga cccgtcctat atgcgcagta tcaatagtct ggccaccttc gttattcaaa
     2101 agaaaaacga ttaactactt attaaagaat taattatcag gacacgttta tgaaatctct
     2161 tgctgaattt cacttgaaaa ataatgcggt aatgggggta tataacaacc gtaccctacc
     2221 ttcttcttat cacgatgcta tgaccgaata taaagccgtt cgtgaaaacg cgctgctggt
     2281 ggactattct cacctctcta ttgttagcgt catgggcgat gatgcctggg cgctgatcaa
     2341 ccagctggtt tccgcagatg tctccatcat tcgtgatgag caggcaatct actcactggt
     2401 gttgaatgaa gagggcacca ttcgcggaga cgtctatgtg ctgtgcagca ttgacggtta
     2461 ttacctgctt tcagaagata tttcggctgc cgaattgatc gccagcatga ataccattct
     2521 cgaaaaagcg gaagaactgg atattcaatc gatgcctgaa attcaggata tgcgggaaaa
     2581 caactggggg gcaatcctgc tcgaaggccc atactcctgg gagatcatgt ctgaaattca
     2641 tggctttgac gtgattggcc tgccttacta cgaatacatg aataccgagg aggatctgct
     2701 gctcttccgc tgtggtaaac acggtgagta tgcgtacatg accatcggtg agcaggcgaa
     2761 actggcggag cagtgggaaa aactgttaac cgttggcgag aaatacctga tgcagaccgg
     2821 cggcctggat tatcaaaaaa tcgtacgcct cgaaaatccg tgctgggatg ccagcctctg
     2881 ggaaggtcag gcggtgaatc cggttcagtt gcagatgcag tgggcggtgc agtacgacaa
     2941 agatgatttt attggcaagg acgcggtgac agagctatcc caggagtata ccggtaataa
     3001 actcatcggc atgatcgcac aggaagaatg cgaaggcatt gaggctggcg atcgcgtgct
     3061 ggtggaaggt caagacgtgg gctatgtcgt gaaggcactc ttttccccag cgttgcagcg
     3121 tttcattgcc ctgaccctgc tggaaaagga ttacgcctgg tctgacatca gcggctacga
     3181 aattcaaact gctcacggaa ttattccggc gcaatcaaaa tgtatgccgt ttatttataa
     3241 cctgagcatg ttggttagcc caactgagca cagttatatc gacgcgtcaa aaaataaaag
     3301 cgcagcgtaa tgaatatcgc cgcgtgacta tagcttacgc ggcttaaatt aaccccaatt
     3361 ggcgggattg ccaggtatta ttcatgaaaa cgaacaggaa aagagttgtt attaccgggc
     3421 tggggatttt gtcctcactg gcggaaaata ttcaggattt ccgggaagca ttattaaata
     3481 aaaaaaatgg cgttaaggat agcgaacgct ttgcaaaatg gtttgaaaat gcgcgtgcgg
     3541 cagaagtgtt gcatgacatt gaatactccg atctgaacga agatgttatc gattcactgg
     3601 ataacgcagc gctctgggcc tacaaagtgg gtaaagatgc gctgagtcag gccggcctga
     3661 tggatagccg tgacaccctc aaagaaacag gcatgatcgt cggtgtttcg tccgcgggaa
     3721 cagaggcatt tttgccgctg ttcgaacagc gcatggaaga tttctcgctg cgtaaagcac
     3781 tcttttccgg cgcattctca tcctgttgtt ccagcgtttc aacgctgctg ggcttacagg
     3841 gtggggttga actggtggcg accgcgtgta ccgccagccc gaacgccgtg gggatggcct
     3901 ttgactacat ccagaatggt aaaagcaaaa ccatgctggc agtgggcact gagcccatct
     3961 atctgccgac ctttgccgga ttctacgcac tgaatgtcat gcatccagac acttgtacgc
     4021 cgttctcggg tcagtccggt atgtcgattg gtgaaggcgc tggcgctatc gtgctggaag
     4081 agtatgagca cgccgtagca cgcggggcga ccatttatgg tgagatcctc tcttacgcca
     4141 cttcctgcga tgctttccat gaaaccgggc cagatccgcg tgccagcgga gcggtgcagg
     4201 tcatgaacaa ggcaatggca aatgcgggcg taaccccgga gcaaatcgat tacgtcaacg
     4261 cgcacggtac gggtaccgaa gccaatgacc gtattgaaac gctggcaatg aaaaaggtct
     4321 tcgctaacca cgataaactg ctggtgagct ccaccaaatc ttactttggc cacaacattg
     4381 gcgcagcggg gattgtggaa ctgattgcct gtctggcaac gcttccggat aaccgcgtac
     4441 tgccgacgct gaatttcagc catgcacgcc cgaattgcga tctggactat gtgccgaatg
     4501 agtttcgcga tcgcaaagtg aacatcttca tgaagaataa ctatgccttc ggcggcaaca
     4561 actgctgcat gatcctgagc atggagcccg cgtccattcc ggtcagtacc tatgattcga
     4621 agcgtgtggc tatctcgggc gtcggtgcag tatccgcaat aggccacacg ctcaaccagg
     4681 ttctggaaaa catctgggcg caggaccacc acgttgaact gggtagcgta accttcccgg
     4741 acgatacact ggaagaagcc aaagagctgc tggccgtgct ggatgaatca aaccagtttg
     4801 ccgagctgtt tgatgaagag tacagcttca gcgaagctac gcttccggaa acggaaagca
     4861 actttaaaac gttccaggtt tccgggctgg agccgcgtaa acacctgcgt cgtttcgatc
     4921 agcgtaaagc gacccgtggc ggcacgtttg ccctgatcgc actcaccgaa gcgctggaac
     4981 aggcgaagcg taaaatcaaa cgcgacggcg atgagttggg catgatcatg gggatgtccc
     5041 gcggcccgca ggaaaccacc tacaaatatc tgcaaagtct gaaacccgat ccacgcaaag
     5101 tacgtacatc ggaatttcct ggctcgctga tgaatgcgat cccaacgttc tgcgggatct
     5161 ctgaagggat caaagggtat accactacgc ttgcgacagg ggaaaactcg gcgctgggcg
     5221 cgctgaccta cggttatgaa atcgtccggc agaacctgca accgcaggta ttggttggcg
     5281 gcgcggatga gtacttcccg tcgatgtcgc tgtatatgga tgcggtgaca caaaagatcc
     5341 acatgacggc tgaggcgagc gactatcaga tttacggtaa agatccgcaa ggttatgttc
     5401 cgggcgaagg ggcttgcatg ctgctgctgg aagatccgca gttggctgtg gcgcgtggcg
     5461 ctgaggttct cgcggaagtg gtgggctacg gcaagtcatg cagcaatacc tacttcgacg
     5521 cctcattgtt ggaagaaaaa tccgcgtcga tggcgctggc cattgaacgc gcccttacgc
     5581 atgcgggcat ctctgctggc gatattgatt tggtatgtgg taccagcaac ggtagcgatg
     5641 accattctcg tatcgaaatt gacgcgattt acaacacctt cgccgcgcaa aacccggcgg
     5701 tgccggtagt gaactataac gcttgctttg gttttgtcgc ctctgccgct ggtttgctta
     5761 acctcgccgt gattactgac tgcattaaga agcaggcagt acctgcgatt ccaaatacgg
     5821 tggaattctt tgatgagcgt gtgaattttg tacgccagtc gatgaaactt gcgctgaacc
     5881 atgtcctgct tgttggcgca acggaaggcg gcaactacta cgcgttcgtc attaagggat
     5941 aagactgtgg acaatgccgc tattatttcg ggttacagcg tgtggatgcc gtatgccgaa
     6001 gactgttcgc agttaattga caatctgaaa caggggaaac gcgttgcgcg tacaccctgg
     6061 tttacctcca acgaggaagc cattaaatgc gggtttaagg gtaatccgtc agtggcgacg
     6121 ttgaaacagg tggacgatag cgcattggac ctgctgtctc agttgatcga cgaagccctg
     6181 gaacaggcaa tgctggataa gcattgcctg gccgggcgca acgttcgcgt ttatctgacc
     6241 ggtattgggc cgcgtatcga cgggctggat tacaaatctt tctataatta caacgatgta
     6301 gaagatatca acttaacgca atccatcaca aatctgcatg cctcaaagat gtcgcaggac
     6361 acgatttcaa gccatcttgc gcgcaaatat cgcttgcagt atttgccgcc taacatgaac
     6421 tgcaccagta actcgtcact gactgcggtg catctggcga cgcagggcat tgagcagggc
     6481 gggattgatc tcgcgattgt gctgaactgt tcgaaaatta aaacccagga catctggttc
     6541 ctggaaacgc aatcgatgct ggacagcgaa caagtgcagc cgtttggtga aaacagtaaa
     6601 ggtgtggcgt tcgcagaagg ttttagcgcg ctcctactgg aaagcgctca tcaccgccgg
     6661 gcgcgtcaac aaagtgaggg cgtaagggtg caaacgactt acacccaaat cagcgcgggc
     6721 cgcagcaacg atgcctcctg gcttagcact aacgtgcaca aggtcatgca ggccgcgatg
     6781 aagcaggctg aaattgcgct ggatgatctt gcagccattc ttccacacgg caacggttcg
     6841 gcagtgagcg ataacgcgga ggccaaagcc atcgccatgt tcgcagggga gcgacagatc
     6901 cccgttctcg cctataaagg gcagatcggc tataccgcaa ccggatctgg tgttgtcgat
     6961 ctgatcattg gccaccattc gttgacgcat catcaactga tcgcacctgt tggcaacgac
     7021 gtcattatcg acagcatggc ttcgctggta cttacggacg gcagcgtgac aaaccatagc
     7081 aaacgccatt tgctgaaggt tggtgtgggt gtcgatggtt cagttattgg cgttgtcatg
     7141 acaaatatgc aagcggggcg cgcgaaatga cggaaaagag tatctacctg tcttcctggg
     7201 cgcttacgga accggacgac attttttatt tttatcagcc gacctggctc aacgcctggg
     7261 aagcacatat gtacgaaagg ggattatacg gcacccgtgc taatggctgg ggactgaagc
     7321 gctttggcgt ggagaacgat atttatgcac cagaacggat ccgtaatcca caatatatgc
     7381 cttaccttga ggcgatggag caggtgaccg attctgcatt acaacaaatg ccgactaaag
     7441 aacgcatgaa gttaatgaaa tcgcgcacgg catttattta tgccgattcc tggggcgaat
     7501 ccggttcgtt tgaaaaaatt tccagtgcgc tgcatatcgc cactattgat acgcttccca
     7561 agaatttact taagaagttt tcgataacgg aaccgacttg caagattcgc ggcgaaaaac
     7621 aggcattcgt tcaggcgctg cgtatggcgc aggattatct cgcctgggat gtattcgata
     7681 acgtggtgat ttgtgctgcc tatcgggcaa ttccggtgct ggtattctct gaagaagatg
     7741 tgggtaaacc gcctaagcgt ttatttcccc gtcgtgctga tgaggtgaat ttatctgttg
     7801 aacgagtggg ttgttttatt ctcagccagc gtgagagcgc catcaggctg caatgcgggg
     7861 aatatcattc cggcactgcg ccacagcatg ctacggatat tgatgtcatg gccttcagcg
     7921 aacggcaaca aatgccgtcc cggcctgctg ctggcgttat cgacctggtg gcccattacg
     7981 gtgaaagcgg ttgtctgacg cccgcgttga gtatggatta tttaacccgt catattcagc
     8041 ccggtggaaa aatgcgtacg gtcattgccg ataaacagtt tggttatcac tactttgacc
     8101 ttgaatacct tgaaggataa cgacagtcgt atgcaatcta ctattaaatc gttgcccgct
     8161 atttcgctta gcaatattta taagtgttat ggcgctgggg caggaaaggt tgatgcgctt
     8221 aaaagcatta atcttgatat caataaaggc gaatttctcg cactgtgtgg gccgtcaggc
     8281 agtggtaaaa gtactctgtt gaatattctc tcgggtattg ataaaccgac cagcggaacg
     8341 gtgatatttt taaataaatt actgaaccaa ctgccagaag agcagttggc tgtgattcgt
     8401 ggaaaacatt tgggttttat ttttcagttt tttaacctta tgccggtatt aaatgttttc
     8461 gataacgttt atttcccact ggtgcttaat ggtcagttca gtaaaaaaga agccactgaa
     8521 cggacgctgc attatctcga tagtgttgga ttaagcggtt ttggcgatcg taaacctggg
     8581 caactatcgg gcggtcaaca acagcgtgtc gccatcgcca gggcactggc ccacgaaccg
     8641 atggtggtca ttgccgatga accgacggga aatttagacc tggcaaccgg cgaggctatt
     8701 ttggacctgt tgttgacgat taatcagcag accggcacca catttgttat ttccacgcac
     8761 tccagcgaat taaaagcgcg ggcacggcga gtggttgaaa ttcaggatgg agtattgatt
     8821 catgactcag atgcataaag gatttttgct catcggcgtc tttttgtacg cggcgtttgt
     8881 ggctccgaag atttacgcgg caacacccga cgctgttaac caggttctgc aacaggggct
     8941 cccggcgcag cctgccgcgc tggcgacgct gaccgaggcg ttaaagaaag agtatgaagc
     9001 caggcacaat gtggtggcgc tggtttttta cgcctatggc ttactgcgtc aagcggacgg
     9061 ttatgcggca acaaacgatt ttatccatgc ctcagaatac gccaaaagcg gctttttctg
     9121 gctggatgaa gcggtggatt tgcaggagaa aaatcaacgc gtacggtatt tacgcgcccg
     9181 cgtagatgcc tatttacccg cagactcagg gcgttgcgtg gtgacagtca aagacaccga
     9241 acacatgctg gctgacccgg cgatatggac gacaacgatc cgcgaccata ttctggcgat
     9301 gcgttatcgc gcattacgtc actgcaagga caccacccgc gcgaatgcgt tgttggcgca
     9361 aattaaaggg caaaacgccg cgcttgcgca gtcgctgacg caggatttta atgttgtacc
     9421 agaatgggat agtgaagagt taacccaggt actgctgccg ctgatgaaag gtgagtaaat
     9481 gtccggggaa attttagctg ttaattgcgt tctgctgctg attctgtttt ttgtgtttta
     9541 tcagcttctg ttttattcac gagatgtcaa atttgcattt ttgaatctct ttcgccacaa
     9601 gcgtcgctct ttttctacca taaccgctat tgtattgggt ggcgtggcaa tttttttata
     9661 tggcggattt attgattact ctttctggat cctgaaagag cagacgattc gtaccaatat
     9721 tggtcatgta caaatttaca atcagcacta ttttgaaacg tcgaataaaa ataagagcct
     9781 gattgccgat tatgcggcgt taaagaaagc tattttgtcg gacccgacgc tggcggcgga
     9841 tatttccacg ctctccgggc aactggagtt tactggcgta atttcacatt acgagagcga
     9901 aacctccagc tatttctccg cgctgggggg cgagccgtta cccgctttaa agctgggttc
     9961 gttcgataag attatcgcgg ggagcgatct ttcccgggta aaaggtgatg aaatcaccct
    10021 gggcagcggt ctggcgaaaa cgttgaatgc gaaatatgac gactggctgg atgtgatggt
    10081 ggtcaacacg gcgggcgggc agggcgcatt gtcgctaaaa atgcgtggca tttttgagtc
    10141 aggcattaaa gattatgacg atgtcgcgat gaagatcccg ctggacaccg cgcagcgcat
    10201 gatgggtacg gatggcgtca gcaaagtgct tattctgctt aaagaggatg acaccgcggc
    10261 gtttagcgca aagctgcgtc aatttatcga acgcaatcat ttgccgctgg tggtcaaaga
    10321 ctggcgtgat gtttcactct tttatcaaca ggttgaaggg ttgctatcgg ggatctattt
    10381 cttcatcaag ctgattgttg cgctgattgt catcttcatg atcggcaact cgatggcaat
    10441 gaacattgtt gagcgcaccc gtgaaattac cactctgcgt gccataggcc tgaagccgct
    10501 tcacgtcacg cgcttgtttc tgacagaagg catttttatt ggtgtgattg gcgcagtcgg
    10561 cagcctgctg gtggggggcg tgttagcctg gattattaat ctttatggta ttgcgatgcc
    10621 gccatcgccg ggacaaacca tcggctatac cgcctttata aaaaccagta acccggaatt
    10681 gatttgggtg acggtggtat tacctatttt gacagcaaca ggtgcctctg tgttaccggc
    10741 tttacgtgct tcccggctga atatttctga cgcatttaaa ttctcatagg gctaattatg
    10801 aatatggctt tttacggcaa gtggtttgct tgcctgtggc tcgctacgtc gtgcgttcag
    10861 gcggcgagca cagataataa ggcgctggag ataattcgtc gcgctgacga aattcgttcg
    10921 ccgaataaac cgtttcgtta cacgctgacg gtaacggaat ataaagcggg cgcaacccag
    10981 ccagagaata aacaggtcct cgatatttcc atgcgcttta tgaaaccgca gggtaatgaa
    11041 aaagcggatg cacgctcgct ggtgcgtttt atttatccgc cgcgagataa aggaaaaatc
    11101 atgctttccg actggtacga tttatggttt tacacgccgg aactgcgacg tcctatgcct
    11161 atttcacggc aacaaagatt aattgggcaa atctctaatg gcgatgtcat tgtcactaac
    11221 tttgaatatg cctatgactc gaccctgatg ggggaagtca cgtgtgctga aaagcagtgt
    11281 tacaagctgg cgctggtgcg taaatcggcg gatatcacct ggccgaaggt tatctattac
    11341 gttgaaaaag acggtgataa tcgcccgtgg aaggcggcct attattcgca ggatgaccag
    11401 ttaataaaag aagtgttgta tcaggatttc cagccggtgt tggggaaaac gcgacccatg
    11461 aaaataaccg ttaccgatgt tcgtcacggc aataactatt cagtgatgga gtacagcgat
    11521 gttcgtctgg aatcgctgcc ggagtttcat ttcaccaaag agtatattca gcgaggtgcg
    11581 aaatgaaacg tacgctgaag atctcttcgt tgctgtgtgt tgcgctgccg ttgacggtac
    11641 aggcggattg cctgtcaggt gacgaggtgg cacaaaatag cgatgtgacg ctttacgagc
    11701 aggtgtctta tgtcaataaa caggcttcgg cctggcagat tgcaggcaaa aatccctaca
    11761 cccgtcacaa cggttatcag gaggcgggga ttggcatcaa tagcggttgt tcgataatcg
    11821 acaatacgct ggaccttaag ctcaatctgt atggaataaa cgaatatgcg cttaaacccg
    11881 cagggaaatt cgaaacggat gatagccgca cgcgtgcgtt aattaaccgc ctcagtctgg
    11941 tctatagcgc ctcggacagc gttcaatttg aagcaggcaa atttgccgcg ccgtcaggaa
    12001 tgttcttttt gcgttcgcct tctgatttgc aaacgcatta ttacaccgga ttccagtcga
    12061 cgcgtttgca cgatcccaaa atgacatcgg cgtatcaggc gtcatcgtgg ggcgcaaaaa
    12121 tgagcgtgga tacacgcgat tatgcgtttt cggcgagtgt cataccgaag ttagccacca
    12181 tcgacaaacg ctatgtgacg tctggcaact ggtcggctaa tcagcaggga aacagcgacg
    12241 aagcctatct gctgagcttc agcgatcatc gctttggtga gcacacgccg acagtcaatg
    12301 tgcggctcgg accatcgccg tcactcgcgc tgtctgacag ttatcactac accccgcagc
    12361 ttacgctgaa tgtcgacgca gcctaccacc gctcccagca atggcggcat ctttcccacc
    12421 gtgagacggc gcaggtcgag gaatatcagt ttccggattc gctctatgaa acgaaagatg
    12481 aaagcggcgt agagctggcg ctgggtgggc agtacaccag tgataatttc agcgtgtttg
    12541 gcgttgagta ttactttcag agcgagggtt attcgcgcgc tgaacagcgt cagcagcgcg
    12601 agttaattga cttcctcaat accacaaccg gctatgcgcc actggatcag gcatttgatt
    12661 cttacaaata cctgatggct tcagaaatca gcaataccgc caatcagggg atgctgcagg
    12721 gtaagcacta tctgaacgcc tgggccagtc tgccgctggc gggagagtcg acgctgcagc
    12781 cttcgctggt ggtgaatctc gttgatggca gcacgttgct gggcctgcat tattccacgc
    12841 cgctcagcgc tataagtaac cagcttgaag cctatgccgg tggctatagc gcgctgggaa
    12901 gtcggtactc cgaatttgcg ctttttggcg acacgttagg cctttatttg ggctttaaat
    12961 actatctata aggataaaga gatgatgaat aatgcttcac aaccgacagt actgcttttt
    13021 gccggtcagg gtaatccggt tattggtatg ggcagcgatc tttgggatct caacgccagt
    13081 acaaaacata tctgggattg tgccagcgat atttccggac tgaacctgcg tcgcctgtgt
    13141 cagaaagggc cgatgaatcg ccttattcaa acgacggtac aacaactggc ggtgaccgca
    13201 attaatgtaa cgctctatac cctgtgccgc gagcgtcttg agaatttaac ggttgtgggc
    13261 tcctgcggac acagcgttgg tgaatacagt gcgctctacg ccgctggagc tatttctctt
    13321 gagacgctat ttggcatgat tcacttcaga gccagcctga tgcatgacga aagccagaga
    13381 aataaaggcg caatgctggc ggttaaaggc atcaaccgcg aacaactgca gacgatgatt
    13441 acggaatcag gcattgcgct ggatattagc tgtgataaca cgccgcgcca gcaggttatt
    13501 gggggaacgc aggctgcgtt gaatgaattt gccacgctgc tgatggccgc tggatacgaa
    13561 ccggttaaac tcggtgtgag cggtgcctgg cacacgcgtt tgatggaaga cggcgttcag
    13621 gcaatgcgcg attatcttgc cggactggat atcgcttcgc cagagcatca ggttctgatg
    13681 aacgtaacag caaaaagcga ggtcgctccg tcaattatca aagaaaatct ctcattacac
    13741 ttaactcata cggtgaaatg gactgaatct ctcgatacat ttctgaatat gccaacctcg
    13801 gtagctttcc ttgaaataag taataagccc tatttgggga atatgttcaa cgatttcgct
    13861 ggcgtggatc agcaacgagt tatgcattgt cgtaaagcat ttagtgatgc gaaggtattc
    13921 aaatgattcc tgattattta acttttattc gctttcagga taaacgaagt ctgatataca
    13981 tttatgctat tggacttatt ctgataggct tttattggaa aaatgcagga tttacttttc
    14041 catcagagga tattggtgtc gttagtggga ttctggccct ggtgctgtat aattttattt
    14101 ttgatctcaa ggcgtactgg gcttataaat gcgtcacgaa gaatatcgat ttttcgtggt
    14161 ttaagaaaaa gcagaaccac aaaatagaat tatttcttac gcaacctctg gtggcaggat
    14221 ttctgtcgtt aatcatgttg agtgcaatga gttgggggct ataccagcgt ctaccctcgt
    14281 tatatgcgct gttcctgatt tcgttacttg ggccgttggt tatctttctg ctgtttcgga
    14341 tgatccgcac cagttatgtc aagcaggtcg ctatttcagt agcgaaaaaa gtaaaatata
    14401 aaagtctgac tcgctatgtg ctgctttcgg tgtgcatctc aacggttgtt aacctgctta
    14461 ctatcagccc gttgcgtaac agtgattctt ttgtgataga ggggcagtgg ttaacgttta
    14521 aatcgataat tgcattgctc attctttgtg gcgtagtgtt ggcgattaat ctgttttttc
    14581 tgcgcttctc caggcggtac gcttttttgg gcaggctttt tttgcaggaa atcgatctct
    14641 ttttctccag tgaaaatgca ttgtcgacct tttttgccaa gccgctttgg cttcggttat
    14701 tcatattgct ggttattgaa gtgatgtgga ttacgctggt gtcggtattg gcaacgcttg
    14761 tggaatggcg gatttggttt gaagcctatt ttttactctg ctatgtaccg tgcttaattt
    14821 actatttttt ctattgtcga ttcctctggc ataacgattt tatgatggca tgtgacatgt
    14881 atttccgttg ggtgcatttt aataagtgag gaa
//
LOCUS       DBFIFS010000111        14828 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.111, whole genome shotgun sequence.
ACCESSION   DBFIFS010000111 DBFIFS010000000
VERSION     DBFIFS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.111"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            394..1530
                     /locus_tag="N7A07_002918"
                     /pseudo
     CDS             394..1530
                     /locus_tag="N7A07_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1524..1661
                     /locus_tag="N7A07_002919"
                     /pseudo
     CDS             1524..1661
                     /locus_tag="N7A07_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(1663..2274)
                     /gene="emtA"
                     /locus_tag="N7A07_002920"
     CDS             complement(1663..2274)
                     /gene="emtA"
                     /locus_tag="N7A07_002920"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU2477547.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2374..3288
                     /gene="ldcA"
                     /locus_tag="N7A07_002921"
     CDS             2374..3288
                     /gene="ldcA"
                     /locus_tag="N7A07_002921"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU2477548.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            3384..5120
                     /gene="cvrA"
                     /locus_tag="N7A07_002922"
     CDS             3384..5120
                     /gene="cvrA"
                     /locus_tag="N7A07_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU2477549.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5509..6579)
                     /gene="dadX"
                     /locus_tag="N7A07_002923"
     CDS             complement(5509..6579)
                     /gene="dadX"
                     /locus_tag="N7A07_002923"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU2477550.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6589..7887)
                     /gene="dadA"
                     /locus_tag="N7A07_002924"
     CDS             complement(6589..7887)
                     /gene="dadA"
                     /locus_tag="N7A07_002924"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU2477551.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8250..9782
                     /gene="ycgB"
                     /locus_tag="N7A07_002925"
     CDS             8250..9782
                     /gene="ycgB"
                     /locus_tag="N7A07_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU2477552.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(9834..10553)
                     /gene="fadR"
                     /locus_tag="N7A07_002926"
     CDS             complement(9834..10553)
                     /gene="fadR"
                     /locus_tag="N7A07_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU2477553.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            10775..12316
                     /gene="nhaB"
                     /locus_tag="N7A07_002927"
     CDS             10775..12316
                     /gene="nhaB"
                     /locus_tag="N7A07_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU2477554.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12462..12992
                     /gene="dsbB"
                     /locus_tag="N7A07_002928"
     CDS             12462..12992
                     /gene="dsbB"
                     /locus_tag="N7A07_002928"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU2477555.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(13038..14306)
                     /gene="umuC"
                     /locus_tag="N7A07_002929"
     CDS             complement(13038..14306)
                     /gene="umuC"
                     /locus_tag="N7A07_002929"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU2477556.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(14306..14725)
                     /gene="umuD"
                     /locus_tag="N7A07_002930"
     CDS             complement(14306..14725)
                     /gene="umuD"
                     /locus_tag="N7A07_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU2477557.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         3506 a         3839 c         3699 g         3784 t
ORIGIN      
        1 tattagtaat gcaaaaatgg cgcatattgt ttttgttatg cagatacatc aagcgaaaat
       61 cccataacta attaggatta ttaccaaaaa tattcaccaa attatttaac cgctgtttgg
      121 tgtaatgttt ttgtcatatt aatagtaacg aattggaata agtaacgaca acttagggaa
      181 taactcgatc taaactcacc tttgtaaggg ggatgttaaa tatatattaa tttctgtgtt
      241 tctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      301 caaatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      361 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      421 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      481 tttggcgcta ttggtttgaa tgcaattctc ttttcccccc tgctgatagc tgctgataca
      541 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac tggagatacc
      601 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      661 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      721 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      781 ggtcaaacct ctgccatcgg cattaattta attggtgact atacccatgc tgacttaggc
      841 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      901 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      961 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
     1021 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
     1081 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
     1141 gtcgatctcg gaacaaacag ttccattaaa accagtggcg ataatgcaca cggcctctgg
     1201 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1261 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1321 cagggcggtg gtctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1381 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1441 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1501 ggtttgtggg cgctcagcgg cgcaagaatg aaaccatcac cactccccgt ctgagcttcc
     1561 gttttcttaa cgtcagcccg acggtggagc ggcaattaca gcggattatt ttctctctcg
     1621 agcgggaagc ccgggagaaa gcggacaaag tacgcgactg atttacatcg cgtccagtgc
     1681 ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca ggatgatttc gcgctacgtg
     1741 atcgaggaac tcgtcagcat ccagatcgtt gattttgctg attgcctttt tccgatctga
     1801 cgagaaagtc cgtagcagcg cacctgcccc gttagcatat gacaccacca gcgcatattg
     1861 cagtaccttc ggatcttcaa tgcctgccag cgggccggtt tccagaatat tcaggtaagc
     1921 cgcccccata gaaatattac gctccggatt tttcagctcg ctggtcgtcg gctcaccact
     1981 ccagcccata cgccgataaa catcacgtcc ggaggttgaa gcttttaact gcatcaaacc
     2041 aatggcattc gatttactca ccgcgttcgg attaccaccc gattcgatag cgataatcgc
     2101 cgtgatcaat tgtggatcga cgccccaggc tgcaccggct ttctggctta ttggcatcca
     2161 ctgcatcgca cgttgcaccg gaactttcgc gttccacggc gggttcgtat agtcatgctt
     2221 tgatgaacaa cccgctaata acacaatcaa aaaggcaaac catctcaatt tcacttcttc
     2281 tatccttata gcctggaatc gccgttgagg tgacaacggc acacctaagc ggcatgatat
     2341 acatttcgta tctgttgtca gcaaggaatt gccatgtctc tgtttcactt aattgcccca
     2401 tcgggttact gcattaaaca gcacgccgcg ctgcgtggta tccagcgcct taccgacgcg
     2461 gggcatcaag tcaataacgt cgaggttatt gcccgtcgtt gtgagcgttt tgcgggtacg
     2521 gaaacggagc gtctggaaga tctcaattcc cttgccagac tgactacccc caacaccatc
     2581 gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac tggcagatat tgactggcag
     2641 gcactggtgg ctcgccaaca atatgacccg ttgctcatat gcgggcatag cgattttacc
     2701 gccattcagt gcggtcttct ggcgcaaggc aatgtcatca cctttagcgg ccccatgctg
     2761 gtagcaaact ttggcgcgga tgaactgaac acctttaccg agcaccattt ctggctggcg
     2821 ttacgcaatg aaacctttac cattgaatgg caaggggaag gcccgacatg tcgggccgaa
     2881 ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac tgatcggtac accgtggatg
     2941 ccaaaaattg agaacggtat tctggtgctg gaagatatta acgagcaccc tttccgcgtc
     3001 gaacgtatgc tgttgcagct ctatcatgcg ggaattttgc cacgtcagaa ggcgattatt
     3061 ctcggtagct ttagcggcag tacgcctaat gattatgacg caggttacaa cctggagtca
     3121 gtgtacgcat ttttgcgttc ccgcctgtcg attccactca ttaccggcct cgattttggt
     3181 catgaacaac gcacggttac gctgccgttg ggcgcacatg ccattcttac taacacccgg
     3241 gaaggcactc agttgacgat tagcggtcat cctgttctta aaatgtaaaa aaaatcacgc
     3301 ttcagggcta agtaaaacgc tgtctctgcc gctaatatgg tagggtttat ataagagaca
     3361 gcgtaatcag gagtaaccga ccgttggatg ccacaacaat aattagcctt ttcatcttag
     3421 gatccatcct tgttaccagc agtatattac ttagttcatt ttcttctcgt cttggcattc
     3481 ctattctggt tatttttctg gcgatcggca tgctggcggg agtcgatggc gtcggcggta
     3541 tcccatttga taattacccc ttcgcctaca tggtaagtaa cctggcgctg gcgattattt
     3601 tgctcgatgg cgggatgcgc actcaggcca gttcctttcg tgtggcgtta ggaccggcgc
     3661 tgtcgctggc gacgctgggc gtacttatca cctctggttt aaccggcatg atggcggcgt
     3721 ggctgtttaa tcttgatttg attgaaggct tattaatcgg cgctatcgtc ggctccaccg
     3781 atgctgcagc ggtcttttct ctgctgggtg gtaaggggct taacgaacgt gttggctcga
     3841 cgctggaaat tgaatccggc agtaatgatc caatggcggt ctttctgacg attaccctga
     3901 ttgcgatgat ccagcagcat gaaagcagtg tcagttggat gttcgtggtc gatattctgc
     3961 agcaatttgg cctcgggatt gtcatcggtc ttggcggtgg ttatttactg ctgcaaatga
     4021 ttaatcgcat cgccctgccc gccggtttat atccattgct ggcattaagc ggcggtattt
     4081 taatttttgc tttaaccacg gcgctggaag gtagcggtat tctggctgtt tatctgtgcg
     4141 gttttctgct gggtaatcgg ccgattcgca accgctacgg catcctgcaa aatttcgacg
     4201 gcctcgcctg gctggcgcaa atcgccatgt tcctggtgct ggggctgttg gttaacccaa
     4261 gcgatctgct gcctattgcc attccggcgc tcattttgtc cgcatggatg attttcttcg
     4321 cccgtcctct ttcggtattt gccggattgc taccattccg tggcttcaat ctgcgtgagc
     4381 gcgtgtttat cagctgggtg ggattacgcg gcgcggtgcc gattattctg gccgtgttcc
     4441 cgatgatggc ggggctggag aatgcacgtc tgttctttaa tgtcgccttc tttgtggttc
     4501 tggtttcact gctcttgcag ggaacatcac tctcgtgggc ggcgaaaaaa gccaaagtgg
     4561 tggttccgcc cgtcggacgt ccggtgtcac gcgttggcct ggatattcat ccggaaaatc
     4621 cgtgggagca gtttgtttat caattgagtg ccgataaatg gtgcgtgggc gcggcactgc
     4681 gcgatttgca tatgccaaaa gagacgcgta ttgcggcact gtttcgtgat aaccagttgc
     4741 ttcatcctac cggcagcacc cgactgcgcg aaggcgatgt gttgtgtgta attggtcggg
     4801 aacgcgatct cccggcgctc ggtaaactgt tcagccagtc gccgccggtc gcgctggatc
     4861 aacgcttctt tggtgacttc attctcgaag ccagcgccaa atatgcagat gtggcgctga
     4921 tatatggcct ggaagacggg cgagaatatc gtgataagca acaaacgctg ggtgaaattg
     4981 tccagcagtt gttaggcgcg gcaccggttg tcggtgacca ggtagagttt gccgggatga
     5041 tctggacggt ggccgagaaa gaagataatg aagtgttgaa gattggcgtt cgagtggctg
     5101 aagaggaagc tgaatcttaa atttaatgaa gcatctatcg ccggttacga tgctttgctt
     5161 aacgcagcct gagcctgtat ataaacttgt catttcctga ttttgatatg cacattccaa
     5221 catcagaaga agcctgttaa tcaacattct gaacatgaac ccgaatctgc atcaagggtg
     5281 aagcgtgatt tatctgaagt cgagttcgag tcaacaatga agcgtaagcg ttttatgtag
     5341 aacgcctgtg cggtgagttc aggttccggg gaaatagtgt ccatcctttt aatgggcact
     5401 atcatgggca atggctctgt cacattaaca actacagttg ctgaccagcc ggatggatga
     5461 ttttttttgc aaccagaaga cgttgcctcc gatccgactt acaacaagtt acaccgtcac
     5521 aaccgggacg cgtaacgcca gcgcgcacat caactcatag cccaccgttc cggcagcggc
     5581 ggcgacatca tcaattttga tctccttgcc ccacagctca actggcgtac caatacccgc
     5641 ctgcgggcaa ggcgttaaat cgaccgccag catatccatc gagactgtcc ccaccgtcat
     5701 ggtgcgcacg ccgtccacta aaacaggggt accggtaggc gcgtggcgcg gataaccgtc
     5761 ggcgtatcct gcggcaacaa tgccgattcg ctgctcatcg cgcgcagtat aacgaccgcc
     5821 gtagcccaca cgctcgcccg cttttagcgt ctggacacca ataatctcac tgcttagcgt
     5881 catcaccgga cgtaatccag tattggcgat atcacgccac tgaccggacg gcgaagcgcc
     5941 atacaaaata atgccaggcc gaacccagtc aaaatgcgct tctgggtgcc acagagtcgc
     6001 cgccgaattg gacaacgaac gccgacactc cagcccttcc gccgcctgct caatacgcgc
     6061 catcgcgccg gaaattccat caggatgttc cgcctcggca aaatgcgaca tcagggtcat
     6121 ttcgccaaca ttcgccatcg cccgcaactg ctgccagacg gtaagcacgc gatcgggctg
     6181 gaagcccaac cgattcatcc cactgttcac tttaagataa atatccaacg gtgcttttag
     6241 ccgcgcattt tgcagtgctt tgagctgcca gttgctgtgt acgcaggtgg tcaggcggtg
     6301 ctggtcatac atttccagat cctgagcatg gaaaaatcct tccagcatca ggatcggtcc
     6361 tttccagccg cgctcacgta acgttattgc ttcttccagg ttaagtaatg caaagccatc
     6421 ggtagccccg agcgcgctcc agatacgctc aataccgtgc ccgtaagcgt tcgcttttac
     6481 caccgaccag acgcgcgcgt gcggcgcggc ctggcggaca atggacagat tctgttttaa
     6541 tgcctgcaga tcgaggctgg cctgtatcgg acgggtcatc tcgtttcctt agctgtgtgc
     6601 gccatgtaaa tggcccggac gtgatggcgt aaatccacgg ctgtagcgcg ctacgcttag
     6661 atcctcatat gggatcgctg gcgtgcgacc agagagcaga tcgcttaaca actgaccgga
     6721 accgcaagcc atcgtccagc cgagcgtgcc gtgaccggta tttagccaca gatttttaaa
     6781 gcgtgtacgc ccgacaaccg gcgtgccgtc tggcgtcatc gggcgcagac ccgtccagaa
     6841 agtcgcctgc tcgacatgac cgccgcgtgg atagagatcg cgaaccacca tctccagcgt
     6901 ttcacgacgc ggttgcaaca gctcggtatt aaaaccaaca atttccgcca ttccgccaac
     6961 acgaatgcgg ttatcgaaac gggtaatggc gattttgtag gtttcatcaa gaatggtgga
     7021 taccggcgca ccatcttcct gcgcaattgg aatggtcagc gagtagcctt tcagcggata
     7081 caccggaata tcaacaatgc ctttgagcat cgccgtcgag taagaaccaa acgccatcac
     7141 atacgcatcg gccttaatca cttcatcgcc acacttcacg ccgtagattt gctcgccgtc
     7201 gcaaagcagt tggtcaacgg gcgtattaaa gcggaattta acccccgcct gctccgccat
     7261 ccgcgccaga ttctgggtaa acagctggca gtctccggtt tcatcattgg gcaattgcag
     7321 accacccgtc agtttgtgcg ctacttctgc cagcgcgggc tccacttccg ccaggcggct
     7381 ggattccagc agctgatacg gtacgccggc atcttccagc acggcgatat cgcgggtcgc
     7441 attttcatac tgttgttcgg tacggaacag ttgcagcgtc ccaccctgac gcccttcata
     7501 ctgaatattg gtttcggcgc gcaatgcttt caggcaatca cggctgtatt ccgccagacg
     7561 caccatccgc cctttgtttt ccatgtagtg gctggtgtcg cagttacgta acatttgcca
     7621 catccatttc aactggaact gcgtaccgtc gagacgaacc gccagcggcg catggcgctg
     7681 gaacatccat ttaatcgctt ttaaaggcac acctggtgcc gcccacggtg cagcatatcc
     7741 gggggagatt tgcccggcat tcgcggcact ggtttccagg gcggcccccg gctcccgatc
     7801 aatgacggtg acctcatgtc ctgcctgatt taagtaccag gcgctggcaa cgcctaccac
     7861 accacttccc agtatgacaa ctcgcatagc cactccgata acggtagata cagtaaaaga
     7921 ataataatct aattacatct tgataactca gttgaaaata ttattcaaca tacggctttt
     7981 ttatggtgag ctggctcaca tctccctgac tctgcggcaa tatggcgtct ttagtatctc
     8041 ctgctgaacg ccctttttat ccagcataaa attccgttca gaaacggatt agtggcactc
     8101 tgataataag aaatcgcaaa gaaaaaaatt ctccggcgac gcggttttta acaggtgttc
     8161 tatgcttgaa atgaggtgct ccacacagag agcgccgaca acaatgaggt gctccacaca
     8221 gagagcgccg acaacaatga ggtgcgcgta tggcgacgat cgattctatg aataaggaca
     8281 ccacacgttt gagcgatgga cccgactgga cgttcgacct gctggatgtt tatctggcag
     8341 agatagaccg ggtggcgaaa ctctaccggc tggataccta cccgcaccag attgaagtga
     8401 taacctcaga acagatgatg gatgcctact ccagcgtcgg catgccaatt aactatccgc
     8461 actggtcatt cggtaaaaag tttatcgaga ctgaacggct gtataagcac ggtcagcaag
     8521 gactggccta tgaaatcgtc attaactcta acccgtgtat cgcttacctg atggaagaga
     8581 acaccattac catgcaagcg ctggtgatgg ctcatgcctg ctatgggcat aactctttct
     8641 tcaaaaacaa ttacttattc cgtagctgga ccgacgccag ttcgattgtc gattatctga
     8701 tttttgcccg taaatatatt accgagtgcg aagaacgtta tggcgttgat gaagtagaac
     8761 ggcttctgga ctcgtgccac gcgctgatga actacggcgt ggaccggtac aaacgcccgc
     8821 aaaaaatctc gctgcaagaa gagaaagccc ggcagaaaag tcgcgaagag tatttgcaaa
     8881 gtcaggtcaa tatgctctgg cgtaccctgc cgaagcgcga ggaagagaaa acggttgctg
     8941 aagcgcgccg ttatccgtcc gaaccacaag aaaacctgct ctattttatg gagaaaaatg
     9001 cgccactgct ggaatcatgg cagcgtgaaa tcctgcgtat tgtgcgtaag gtgagccagt
     9061 atttttatcc gcaaaaacag actcaggtga tgaacgaagg ctgggccacc ttctggcact
     9121 acaccatcct taaccatctg tatgatgaag ggaaagtaac ggaacgtttt atgctggagt
     9181 ttttgcacag ccacaccaat gtggtcttcc agccccccta taacagcccg tggtacagcg
     9241 gcatcaaccc gtatgccctc gggttcgcca tgttccagga tattaaacgg atttgtcagt
     9301 cgccaacgga agaagacaaa tactggttcc cggatatcgc cggttccgac tggctggaaa
     9361 cgctgcattt cgcgatgcgt gatttcaaag atgagagttt tatcagccag ttcctgtcgc
     9421 cgaaagtgat gcgtgatttc cgcttcttca ccgtgctgga tgatgatcgg cataattatc
     9481 tggagatttc cgctattcat aatgaagaag gttatcggga gatccgtaac cggctatcgt
     9541 cgcaatataa cttaagtaat ctggagccga atattcagat ctggaacgtg gatttgcgcg
     9601 gcgaccgttc gctgacgctg cgttacattc cacataatcg cgcaccgctg gatcgggggc
     9661 gcaaagaagt actgaagcat gtgcatcgcc tgtggggatt tgatgtgatg ctggaacagc
     9721 aaaacgaaga cggcagcgtc gagttgctgg aacgttgccc gccgagaatg ggaaatctgt
     9781 aaaaacaaca aaaaacccct cgtttgaggg gtttgttttt taaacggaag ggattatcgc
     9841 ccctgaatgg ctaaatcacc cggcagattt ttctgcatcc ggtgccaaat ctcgccactc
     9901 tcatgcccat agcgacgcac tgtttcgtac acctgatcgt gcgcgccttc actgcacaac
     9961 gccgacagtt tgtggtagaa gcccagcgcc agactgcgcg cttccggatt ggcgaaatag
    10021 tgacgaccaa tgcgcgtata cagtcccttc atcccgttaa ggatcagacc gtaaatcggg
    10081 ttgccggaag caaacgccag gccgcggaat atgttgtaat ccagctcggc aaaggcatcg
    10141 gcgtgatcgg ccacttcatt agcggtagcc agcacttcct gcgctttatc gggatgctga
    10201 cgaaacgcgg tgcgaataaa aatagttgaa atattggtac gcaccgacag caaattatca
    10261 ataagctgcg gcacactttc gtgatccagt cgcgccagtg tttcaaggat atttaaacca
    10321 gaggtttccc agaaattatt caccttcgtc ggcttgccat gttgaatggt caaccagcca
    10381 tctcgtgcca gacgctgtaa cacttcacgt aacgtagtac gcgttacgcc aattaattct
    10441 gaaagttcac gttctgcggg caaaatagtc ccgggaggga agcggttatt ccagatactt
    10501 tcaataatgt actcttccgc gaaacccgcc gggctttgcg ccttaatgac catagtgaga
    10561 tttccataac acagcaaaac aaagttggac tcatcatacc agagggctaa ctacgctgat
    10621 agcagacaaa aagaagaaaa aagggatcaa ggcttcattt tccgcatctc tttccccttg
    10681 actatcaaga cttgcctcac tcctgcctga ccgttaagct ttgcgtttaa acataaaaac
    10741 atcattgatt atttgtaagg taagggaaac catcatggag atctcctggg gccgcgcgct
    10801 atggcgcaat tttttgggcc agtcccccga ctggtacaaa ctcgccctca tcattttctt
    10861 aatcgtaaat ccgttaattt tcctcatcag ccctttcgtc gctggctggt tgctggtagc
    10921 ggaatttatt ttcactctgg cgatggccct gaaatgctac ccgctgctcc ccggtggcct
    10981 gttggctatc gaagcggtat tcatcggcat gaccagcgcg gaacacgtcc gtgaagaggt
    11041 ggcggcaaat cttgaagtct tgctgttact gatgtttatg gtggcgggta tctattttat
    11101 gaaacagcta ttgctgttca tatttacccg tttgctgtta agcattcgct ccaaaatgct
    11161 gctgtcgctc tctttttgcg tagcggcagc gttcctctcc gcgttcctcg atgccttaac
    11221 cgtcgtggcg gtggtgatca gcgttgccgt cggtttttat ggtatttatc atcgcgtagc
    11281 ctcttcccgt accgaagaca ccgacctgca agacgatagt catatcgaca agcattacaa
    11341 agtggttctg gaacagttcc gtggctttct gcgtagcctg atgatgcatg ccggtgtcgg
    11401 caccgcatta ggcggcgtaa tgaccatggt gggcgaacca cagaacctga tcatcgctaa
    11461 agcggctggc tggcattttg gcgatttctt cctgcgcatg tcgccggtga ccgttccggt
    11521 tctgatttgt ggcctgttaa cctgcctgct ggtagagaag ctgcgttggt ttggctacgg
    11581 tgaaacgctg ccggagaaag tccgcgaagt gttgcaacag tttgacgatc aaagccgcca
    11641 ccagcgtacc cgtcaggata aaatccgtct gattgtccag gcgattattg gcgtctggct
    11701 ggtgactgcg ctggcgttgc atctggcgga agttggcttg attggtttgt cagtcattat
    11761 tctggcaaca tcattgaccg gtgtcaccga tgaacatgct atcggtaaag ccttcaccga
    11821 atctctgcca ttcaccgcac tgttgacggt gtttttctcg gtagtcgcgg tgattatcga
    11881 ccaacaactg ttttcgccga ttattcagtt tgtgttgcag gcatcggaac atgctcagct
    11941 gtcgctgttc tatattttca acggtctgct gtcatccatt tcggataacg tcttcgtggg
    12001 gacgatttat atcaacgaag cgaaagcggc aatggaaagt ggcgctatca cgttgaagca
    12061 atacgagctg ctggcggtcg ccattaatac cggtaccaat ctgccctctg tcgctacgcc
    12121 gaacggtcag gctgcgttcc tgttcctgct gacctctgca ctcgcgccat tgattcgcct
    12181 ctcttatggc cgcatggtgt ggatggccct gccttacacc ctcgtcctga cactcgtcgg
    12241 actgctctgc gtcgagttta cgcttgcccc tgtaaccgaa tggtttatgc aaatgggctg
    12301 gatagcaacg ctttgataac aacttaccgg gcatataaat gcccggtttg ccttttcgcc
    12361 cgataattgt ccaattgcgt cctatttttg ctgcctcctg gtggcgcagc gaatgaattg
    12421 gtttaaactg cgcactctat gcatattgca gggaaatgat tatgttacga tttttgaacc
    12481 aatgttcaca aggccggggc gcgtggctgt tgatggcgtt tactgctctg gcactggaac
    12541 tgacggcgct gtggttccag catgtgatgt tactgaaacc ttgcgtgctc tgtatttatg
    12601 aacgctgcgc attattcggc gttctgggtg ctgcgctgat tggcgcgatc gccccaaaaa
    12661 cgccactgcg ttatgtagcg atggttatct ggttgtatag tgcgttccgc ggtgtgcagt
    12721 taacttacga gcacaccatg cttcagctct atccttcgcc gtttgccacc tgtgatttta
    12781 tggttcgttt cccggaatgg ctgccgctgg ataaatgggt gccgcaagtg tttgtcgcct
    12841 ctggcgattg cgccgagcgt cagtgggatt ttttaggtct ggaaatgccg cagtggctgc
    12901 tcggtatttt tatcgcttac ctgattgtcg cggtgctggt ggtgatttcc cagccgttta
    12961 aagcgaaaaa acgtgacctg ttcggtcgct aatccattcg gcgctcctgc gggagcgctt
    13021 ttttcctgcc gctgtgttta tttgacccgc agtaaatcag aacttcgcgt tgtataacgt
    13081 ggtgaaagca tggctcgctt catctgccat tgttgctgga tcccctgccc ggcaaaatag
    13141 agtgttcctc tgccctcttt agcattcagt gtatccatta ccgccatcaa ttgctcactc
    13201 ccggggcgcg gagcgttgtc atcgaataaa ttgagctgcg cgactccctg actgaagaaa
    13261 tcccccagca tcacgcccgc tttttggtag cgatggcccg cttgccagat ggcatccaga
    13321 gatcgcgtag cggcgttaat gatatccctg ctgtcctgag tgggcgtcag cagttttacc
    13381 gacgcgctat tgccgtaata aggttcattg agcgcaaatg gtgacgtctt aataaatgtg
    13441 gagataaacc gacaatattg atgctcactg cgaagttttt ccgccgcccg ggcagcgtaa
    13501 ctacaaatgg cctgccgcat cgacgtataa tccgtgatgc gttcaccaaa cgagcgggaa
    13561 cagataattt cctgctttgt cggtgcaaat tcttccagtt gcaaacaggg ttcaccgcgc
    13621 agttcacgca ccgttctttc gagcacgaca ttaaaatgtt tacggataaa ccggatatct
    13681 gtatccgcca aatcgagaac ggttttgatc cccatcgcgt ccagtttttt gctgatccgc
    13741 cgtccaatcc cccagacgtc atccacgggg agagcagaca ttaatttacg ctggcgttcc
    13801 agatttgata aatccaccac cccacccgtc tgccgctgcc atttttttgc cgcatgattg
    13861 gcaagcttag ccagcgtttt ggtctgggcg atccccacac caacagtaag atgggtacgt
    13921 tgtagcaccg ttgcgcgaat ttctctgcca aagtcagtca gatcgcgaca attacgcaca
    13981 cctgtcagat cgcagaatgc ctcatcaata ctgtaaatct cgacgcgggg cgatagttct
    14041 tccagcgtcg acatcacccg attgctcatg tctgcgtaaa gctcataatt gctgctaaag
    14101 caaaccacgc cacagcgacg aaacagatct ttttgtttga accagggatc gcccatttta
    14161 acgccaagcg cctttgcctc agcgtttcgg gcgataacgc aaccgtcatt attcgatagc
    14221 acaaccaccg gtttacccca taactcaggg cgaaacaccg tctcacagct ggcataaaac
    14281 gcgtttacat cacagagggc aaacatcagc gcatcgcctt aacgacgtgg atcaccacac
    14341 caaagacatc cagcgtatct tcactactga tggtaatggg cgagtacgcg ctgttcatgg
    14401 gaataagctg taccgtcggg cgtagttgca attttttcac cgtaaactcg ccgtcaacag
    14461 cagcgatgac aatatcacca tggctggcgg taatagcgct atcgacaatc agtaaatcac
    14521 cgtcactaat tccaccatca atcatagaat caccacttgc tttgacgaag taagtcgcgc
    14581 tgggatgctg gatcaacagt tgattcagat cgatgcgctg ttcaacgtaa tctgcggccg
    14641 gtgaaggaaa gccacactga acaagatcgc taaatagcgg aaaagtcaca atttcgcgga
    14701 gatccgcagg cttgataaac aacataataa tctgcctgaa gttatactgt ttttatatac
    14761 agtagtctgt tcttgccagc agatcaatac tgattcaggc tatcaatatt tgtcgctgca
    14821 tagactgc
//
LOCUS       DBFIFS010000112        14638 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.112, whole genome shotgun sequence.
ACCESSION   DBFIFS010000112 DBFIFS010000000
VERSION     DBFIFS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.112"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..541
                     /locus_tag="N7A07_002931"
     CDS             <1..541
                     /locus_tag="N7A07_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU2477558.1"
                     /translation="GRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCEV
                     PDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPKMTVRYYISSADLTAGKFATAIRN
                     HWHVENKLHWRLDVVMNKDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMR
                     KAAMDRNYLASVLAGSGLS"
     gene            690..1199
                     /locus_tag="N7A07_002932"
     CDS             690..1199
                     /locus_tag="N7A07_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU2477559.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            1196..2614
                     /gene="phrB"
                     /locus_tag="N7A07_002933"
     CDS             1196..2614
                     /gene="phrB"
                     /locus_tag="N7A07_002933"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU2477560.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2764..4245)
                     /gene="dtpD"
                     /locus_tag="N7A07_002934"
     CDS             complement(2764..4245)
                     /gene="dtpD"
                     /locus_tag="N7A07_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU2477561.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            4516..5259
                     /gene="ybgI"
                     /locus_tag="N7A07_002935"
     CDS             4516..5259
                     /gene="ybgI"
                     /locus_tag="N7A07_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU2477562.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5282..5938
                     /gene="pxpB"
                     /locus_tag="N7A07_002936"
     CDS             5282..5938
                     /gene="pxpB"
                     /locus_tag="N7A07_002936"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU2477563.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5932..6864
                     /gene="pxpC"
                     /locus_tag="N7A07_002937"
     CDS             5932..6864
                     /gene="pxpC"
                     /locus_tag="N7A07_002937"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU2477564.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKSRQDQQRYFEQLAWRLHNEN"
     gene            6854..7588
                     /gene="pxpA"
                     /locus_tag="N7A07_002938"
     CDS             6854..7588
                     /gene="pxpA"
                     /locus_tag="N7A07_002938"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU2477565.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7624..8414
                     /gene="nei"
                     /locus_tag="N7A07_002939"
                     /pseudo
     CDS             7624..8414
                     /gene="nei"
                     /locus_tag="N7A07_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
     gene            complement(8411..9457)
                     /locus_tag="N7A07_002940"
     CDS             complement(8411..9457)
                     /locus_tag="N7A07_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU2477566.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9606..10667)
                     /locus_tag="N7A07_002941"
     CDS             complement(9606..10667)
                     /locus_tag="N7A07_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2477567.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AAPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10664..11395)
                     /locus_tag="N7A07_002942"
     CDS             complement(10664..11395)
                     /locus_tag="N7A07_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2477568.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(11410..13869)
                     /locus_tag="N7A07_002943"
     CDS             complement(11410..13869)
                     /locus_tag="N7A07_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2477569.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13919..14485)
                     /locus_tag="N7A07_002944"
     CDS             complement(13919..14485)
                     /locus_tag="N7A07_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2477570.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3606 a         3572 c         3959 g         3501 t
ORIGIN      
        1 ggggcggcta aataaagcct ttgaggaaaa atttccgctg aaagaattaa ataatccaga
       61 gcatgacagt tacgcaatta gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat
      121 tgtttgcgaa gtccctgatg aacttattga tttcacgttt gaatggaaag ggctgaagaa
      181 attatgcgtg gcagtctcct ttcggtccat aatagcagaa caaaagaaag agccaaaaat
      241 gacggtcaga tattatatca gttctgctga tttaaccgca gggaagttcg ccacagcaat
      301 ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggacgtgg taatgaataa
      361 agacgactgc aaaataagaa gaggaaatgc agcagaatta ttttcaggga tacggcacat
      421 tgctattaat attttgacga atgataaggt attcaaggca gggttaagac gtaagatgcg
      481 aaaagcagcc atggacagaa actatctggc gtcagtcctt gcggggagcg ggctttcgta
      541 atcttgccct ggtattcgct gctttgatga tgatgtaaaa tgattgtttt tgaactggat
      601 ataacagctt ggtagggaag aaagcataac cccacaaaat tcatttacca tcgtctacac
      661 ttactcctgt aaaccgttca ggagcagtaa tgaatcagca acgatttgat gacagcactc
      721 taatccgtat cttcgcgctt catgagttac atcgactgaa agaacatggc ttaacgcgcg
      781 gggcgcttct cgattatcac agccgctata aactcgtatt tctggctcat tcccagccgg
      841 aataccgcaa acttggcccg ttcgtggctg atattcacca gtggcaaaat ctggatgatt
      901 tttacaacca gtaccgccaa cgcgtaattg ttttgctttc tcatcccgcc aacccgcgcg
      961 atcacaccaa cgttttgatg cacgttcagg gttattttcg cccgcatatt gagtccacag
     1021 aacgccagca gctggctgcg cttatcgaca gttatcgccg tggcgagcaa ccgcttcttg
     1081 cgccgctgat gcgtatcaaa cactatatgg cgctttatcc ttacgcctgg ctttcagggc
     1141 agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg ccattcagga gttttatgac
     1201 tacccatctg gtctggtttc gccaggattt acgtctgcat gataatctcg cgctggctgc
     1261 cgcctgccgc aattcatctg cacgcctgct ggcgttgtat atcgctacac cacgccagtg
     1321 ggcggcgcat aatatgtcgc cgcgtcaggc tgaacttatc aatgctcaac tgaacgggct
     1381 acaaatcgcg ctggcggaaa aaggcattcc tttattgttc cgtgaagtgg atgactttgc
     1441 cgccagcgtc gaaatagtta aacaggtgtg cgcggaaaac agcgttactc atctttttta
     1501 taactatcag tatgaggtga atgagcgggc gcgggatgtg caggttgaga gaactctgcg
     1561 taacgtggtg tgtgaaggat ttgatgacag cgtgatcctt ccgcctggcg cggtgatgac
     1621 cggcaatcat gagatgtaca aagtctttac gccttttaag aatgcctggc tgaaacggct
     1681 gcgggaaggg atgccggagt gcgtcgctgc accaaaagtt cgtagtagcg gatcgataaa
     1741 gcccgcgcca tccattacgc tgaattatcc tcgtcagtct ttcgatactg cgcatttccc
     1801 ggtggaagaa aaagcggcga ttgcgcaatt acgccagttt tgccagaacg gtgccggaga
     1861 atatgagcaa caacgagatt ttccggcagt ggaaggcacc agtcgtttgt ccgccagcct
     1921 ggcaacgggc gggttatcgc ctcgccagtg tttgcatcgc ttgttggcgg aacagccgca
     1981 ggcgctggac ggtggggccg gtagtgtctg gcttaatgag ctgatctggc gcgagttcta
     2041 ccgtcatctg atgacgtatt acccctcgtt gtgtaaacat tgtccgttta ttgcctggac
     2101 ggatcgtgtg cagtggcaga gcaatcccgc acatttacag gcctggcagg aaggcaaaac
     2161 gggatacccg attgtcgatg ctgccatgcg tcagcttaac agcactggct ggatgcataa
     2221 caggctacgg atgattacag ccagttttct ggtgaaagat ttgttgatcg actggcgcga
     2281 aggcgagcga tatttcatgt cgcagctgat tgatggtgat ttggcagcca ataacggtgg
     2341 ctggcagtgg gcggcttcta cgggtactga tgcagcgccg tattttcgta ttttcaaccc
     2401 gacaacccag ggcgagaaat ttgaccgcga aggcgagttt atccgccagt ggctaccgga
     2461 actgcgcaat gtaccgggga aatcggtgca tgagccgtgg aagtgggctg agaaagcagg
     2521 tgtgaagctg gattatccgc aaccgatagt cgagcacaaa gaggcgagag tacaaacgtt
     2581 ggcagcgtat gaggcggcgc ggaaggggaa ataacagacg cgtcaggcaa tcgagcccag
     2641 atgccggatg cggcgtgaac gccttatcca gcctacaaat ttgtgcaaat tcaataaatt
     2701 gcaggaaata cgtaggcctg ataagacgcg tcaagcgtcg catcaggcat cggtgctggc
     2761 ctattaagac tccagcgcca gcgcgcggtt tctgaatttc agcgcctgat acagccaaat
     2821 catcagtacc actcctacac acgccagtgc gccccaggta atttgatcaa atacttcaat
     2881 atatgcattg atggagtagt tgatcgcccc ggaagcatca aacgaagcct gcgatgtctg
     2941 atcggcaatc acgcccgcca gatagttcgc aatcgcgccg gaaagcagca tataaatgcc
     3001 ggttaatacg ccggtcacac cggggatttc aatgcgcgta atctgcgaca tagcaaccgg
     3061 gtcgataaac agttccgcaa agcccatcac cgccaggcct aataccatca gtggcagaga
     3121 agagtgacca tacattgcgg accagcgggc gcttaaggtc agaatgcaga atccggcgct
     3181 catcaggcca aggccaagag caaattttcc ccagatgcgc acggtacgat tacccgcgac
     3241 actctctttt accacccacg ccaggaacac accgcacagc ataactgcga aggcattaat
     3301 cgactggaac atcgcggtcg gaacggtata accaaacata tcgcgattaa cgaagcggtc
     3361 gatataaagg ctaatcgagc taccgccctg ttgtgcgaag gcccagaaca acatactgaa
     3421 gaaggtgagc gtcacaatca gcctcagctc cttccgctgt ttctggtttt ctgctttgcg
     3481 ataaattttt gccagtacac ccaggccaat gatagtcgcg acaatcaagg cgtataccga
     3541 ccattctttc cagaacagta cggtaatcag cgcaggcgtt gccaccagca gaaccagcag
     3601 ccatccccag ttcggcagca gaaagtttgt cgcacgcagt acttttttgt taacgccgcg
     3661 ggtatgtgtg aaatgacgat tgccacataa gaaaataacc agacccgcga tcatacccac
     3721 cgccgccagg ccaaagccca tcgcccaact gtactcttcc tgggcgtagc cacaggcaat
     3781 aggtgcgata atagacccca cgttacccgc tgcatacatc agcgagaaac cgccatcacg
     3841 acgcggatcg gttggctcat acagctcgcc gagcaggcaa ctgacattgg atttaaacag
     3901 accatagcca caaacgatta tcgccaggga cagatagagg aatgacggat ggatctcact
     3961 tgcacccaac accacatgac cgatcgccat caataacgcc cccagcatca ccgccatgcg
     4021 attgccgaga actttatccg ccaaaaagcc accgaggatt ggcgtgacat acaccagcga
     4081 acagtaggcg ctaaataact cgtaggcgtg agtatcgttg tattttagtt gattggtgag
     4141 atagagaatc agcagggcac gcatgccgta aaagctaaaa tattcccaga tttgcagcgc
     4201 aacgacatag taaatagcgc gcggttgtga tgcgtgttta ttcataataa ttccacaatt
     4261 gacggcaacg tcacgtaata gttgcgcggc gtgcttgtgt ttccttaaag ttaaaacttt
     4321 gatacagatc tgattattta tgcaatatgc tgtctgattg cataaatata cattagctga
     4381 agcgtgatga tataaacaat ttgcgtcttt tggcgtcggg tttgtttctt aatatgtcaa
     4441 aagattggct atcgacgaac aggcgaggta acgctatgtt aacggtgtag ctatcagaat
     4501 acggaaggcg cgaagatgaa aaacaccgaa ctggaacaac tgattaacga aaaactgaac
     4561 agcgcggcga ttagtgatta cgcgccgaat ggtttgcagg tggaaggcaa agagacggtg
     4621 caaaaaattg ttaccggtgt cactgccagc caggctttgc tcgatgaggc agtgcgtctg
     4681 ggcgcggatg cggtcattgt gcatcatggc tacttctgga aaggcgaatc tccggtcatt
     4741 cgcggcatga agcgtaaccg tttaaaaacg ttgctggcga atgatatcaa cctgtatggc
     4801 tggcatttgc cgcttgacgc acatcctgag ctgggcaata acgcacagct ggcggcgtta
     4861 ctggggatca cggtcatggg cgaaattgag ccgttggtgc cgtggggcga actgaccatg
     4921 cctgtgccgg gactggagct ggcttcctgg attgaagcgc gtctgggacg taagccgtta
     4981 tggtgtggcg ataccggacc tgaggtggtc cagcgcgtcg cctggtgcac gggcggcggg
     5041 caaagtttta tcgatagcgc cgcgcgtttt ggcgtggatg cttttattac tggcgaagtt
     5101 tctgaacaga ccattcattc agcccgcgag cagggattgc atttttatgc tgcaggtcac
     5161 catgccactg aacgtggtgg tattcgcgca ttgagcgagt ggctgaatga aaataccgat
     5221 cttgatgtga cctttattga tattcctaat cctgcataac gaataatcag agggatcgaa
     5281 agtgcaacga gcgcgttgtt atctgatagg tgaaacggcg gtagtgctgg aactggaacc
     5341 gccggtgacg ctggctagcc agaaacggat ctggcgactg gcgcagcgtc tggtggatat
     5401 gccgaatgtg gttgaagcca ttcccggcat gaacaatatc acggtgattt tgcgtaatcc
     5461 tgagtcgctg gcgctggatg ccatagagcg tttgcaacgc tggtgggagg agagcgaggc
     5521 cctggaacct gagtctcgct ttattgaaat tccggtggtt tacggtggtg caggcggacc
     5581 ggatttggcg gtggtcgcgg cgcattgcgg gttgagcgaa aaacaggttg ttgaattgca
     5641 ctcctccgtg gaatacgtgg tctggttttt aggttttcaa ccgggcttcc cgtatctcgg
     5701 gagtttgccg gaacaactac acacgccacg gcgcgctgaa ccgcgcttgc tcgttccggc
     5761 aggttctgtc gggatcggcg ggccgcagac tggtgtttat ccgctggcaa cgccgggtgg
     5821 ctggcagttg attggtcata cctcactcag cctgtttgat ccggcgcgtg acgaacccat
     5881 cttattacgt ccgggagaca gcgtgcgctt tgtaccgcaa aaggagggag tatgctgaag
     5941 attattcgtg cgggcatgta taccactgtg caagatggcg gtcgtcacgg ttttcgccag
     6001 tcgggtatca gccactgcgg cgcactggat atgcctgcgt tacgcattgc taacctgctg
     6061 gtgggtaatg atgccaatgc ccccgcgctg gagatcacgc tcggtcagtt aacggttgag
     6121 ttcgaaactg atgggtggtt tgctctgacg ggtgccggtt gcgaagcgcg gctggatgat
     6181 aatgccgtct ggaccggctg gcgattgccg atgaaagcag gccagcgttt aacgcttaaa
     6241 cgcccgcagc acgggatgcg cagttatctg gcggtcgcgg gtggtattga tgttccgccg
     6301 gtaatggggt catgcagcac cgatctcaaa gtggggattg gcgggctgga aggccgttta
     6361 ctgaaggatg gtgaccgact cccgattggc aaagcgaagc gtgattttat ggaagcgcag
     6421 ggcgttaaac agctgctgtg gggcaaccgc attcgcgcct tgccggggcc ggaatatcat
     6481 gagttcgatc gcgcctcgca ggatgcattc tggcgttcgc cctggcagct tagctcgcaa
     6541 agtaaccgca tgggctatcg cttacagggg caaattttaa aacgcaccac cgatcgcgaa
     6601 ctgttatctc acggtttgtt accgggcgtg gtgcaggtgc cgcataacgg gcagcccatt
     6661 gtgttgatga acgacgcaca gaccaccggt ggttacccgc gtattgcctg tatcattgag
     6721 gctgatatgt accatctggc gcaaattccg ctcggtcagc cgattcattt tgtccagtgt
     6781 tcactggaag aggcactgaa atcgcggcaa gatcagcaac gttatttcga acaattagcg
     6841 tggcggctgc acaatgaaaa ttgacctgaa cgccgatctg ggcgaaggct gcaccagcga
     6901 cgcagagcta ttaacgctgg tttcctctgc caatattgcc tgtggatttc atgcaggcga
     6961 tgcgcaaacc atgcaggctt gcgtgcgtga agcaataaaa aatggtgtcg cgattggcgc
     7021 tcatccgagt tttcccgaca ggaaaaattt tggtcgcagc gctatgcagc tgccgccaga
     7081 aaccgtttac gcccagacgc tgtatcaaat tggcgcgctg gcaacgatta cccgtgcgca
     7141 aggtggtgta atgcgtcatg tcaaaccgca cggcatgtta tacaaccagg cggcgaaaga
     7201 ggcacaactg gcagacgcca tcgccagagc ggtatacgct tgcgatccag cattggttct
     7261 cgttgggctg gcaggaagcg agctgattcg tgcaggcaag caatatggcc tgacaacgcg
     7321 cgaggaagtg tttgccgatc gcggttatca ggctgacggc tcgctggtgc cgcgaagcca
     7381 gtcaggcgcg ctgattgaag acgaagaaca ggcgcttgcg caaacgttgg agatggtgca
     7441 acacggcaga gtcaaaagta tcaccggcga atgggcaacg gtcacggcgc aaacggtctg
     7501 cctgcatggc gacggcgagc acgcactggc gttcgcccgc cgactacgct ctgcatttgc
     7561 cgaaaagggg attgttgtcg cagcataacc ccgattaata aagaatgaaa aaaggatatc
     7621 accatgcctg aaggcccgga gatccgccgt gcagcggata acctggaggc ggcgatcaaa
     7681 ggcaaaccac taactgatgt ctggtttgcc ttcccgcagt taaaaactta tcaatcacaa
     7741 cttatcggtc aacacgttac ccatgtggaa acgcgtggta aggcgttgtt aactcatttt
     7801 tccaacgact taacgctcta cagccataat cagctttatg gcgtctggcg cgtggttgat
     7861 accggcgaag agtcgcagac cacgcgagta ttgcgggtaa aactgcaaac ggctgacaaa
     7921 accattctgc tttatagcgc ctcggatatt gagatgttga ccccggaaca actgaccacg
     7981 catccgtttt tacaacgcgt tggtcccgat gtgctggatc cgaatctgac gccggaggtg
     8041 gtgaaagaac ggttattgtc gccgcgcttt cgtaaccgtc agtttgctgg attactgctc
     8101 gatcaggcgt ttctggctgg gcttggcaat tatttgcgtg tggagatcct ctggcaggtt
     8161 gggttgactg gaaatcataa agcgaagacc tcaatgcggc gcaactggat gcactcgcac
     8221 acgcgttact ggatattcct cggttttctt acgctacgcg ggggcaggtg gatgagaata
     8281 agcatcatgg ggcgctgttt cgctttaagg tttttcatcg tgatggcgaa ctgtgcgaac
     8341 gctgtggcgg catcattgag aaaaccacgc tgtcatctcg cccgttttac tggtgtccgg
     8401 gctgccagca ctaggccgta cgtttcggcg cataggttga aataaaccgc gcaatggcag
     8461 gccctgtcag caaaatactg aacaggcgca gggtttgcat cgccataatg agcgccatat
     8521 cggcattgct ccctgcggcg atgaccgcca cggtatcaag cccgccgggg ctggtggcga
     8581 ggtaggcagt cataaaatca atgtgcataa accgggtcag tccccacgcc atacccgcac
     8641 aaatagccag cagagcaaaa atcgacagca ggatttgtgg tagcgggcgt agtgcccgca
     8701 gtaagatttg tttatcgaaa ccaaggccaa tccgccagcc aattgccata tacgccatcg
     8761 ccagtagcca ttccggtagt tcgatggtga tgagctgacc agactggagc actgcgcccg
     8821 ccagcatcgg gatgagcatc gtgccggaag gaagacgcag caggcatccc actgtaccgg
     8881 caacgaccgc gagcaaaata gtaagcagga gattaatgct cactggcggg aaccagacga
     8941 tatgctggtt aaccgcttca gcgttatcgc ccagcatcat tcgcgtgacc agaactgcgg
     9001 cccccgcgac aaacagcact cgcagatact gcataaacgc caccaggcgg atatctgcgc
     9061 cgtaatcctg cgccatcgcg accattgccg ccgccccgcc aggcgaggag ccccaggcac
     9121 cagtatttcc gggcagtgag ctatagcgca ccagtagcca tcccacgata gcgctggaaa
     9181 gcaacgtcac cagtaaaata gctaacacaa tcggccagtt aacctccagc gtggtcagga
     9241 tcgaaccggt caagttttgc gcaatcatgc agccaagaat ggcctgcgcg gcgagaaaag
     9301 cggagcgggg gagttgcagg gtaaccccgc gcatactgaa gatgatcccg gcgatcatcg
     9361 gtccaagtaa gagtgccgcc gggagatgta atccgaggaa accaatagaa aggagaagtg
     9421 atagcacaca taacattccc cactgcaaaa ctggcatcct ttgctccctc attattttct
     9481 ctttggtgta tcgatacagc ataaagaatg ccgggcatgg aaaaaataac ggtaatcaat
     9541 aaaagccgga aaattggcaa ttaaccgaag aagccctctc cggctggaaa gggcaatagg
     9601 ctaaatcagt tgtagttaat tttgaaaagt accactgtat caaagggacc aacgtcgatg
     9661 ggcgtacccg gcgtggatga caattctgcc gtgtaggttt tttcatacac taacaggtcg
     9721 ctggtaaaat cggcatactc actgtatttg ttgaaagtat agggtgaggc attttcatcc
     9781 aacaatcgaa gcttcaagcc attgccaatg agcagtgatt tatcttcgtc gataagggtt
     9841 tcatcggtat aaaacgaaga gctgacttta aaaccgtcgg tacactggtc gttttgtgat
     9901 ttggttgtag ttattttaaa ttgctgcgac atcgtatggc gacgaatatc cagcacgtta
     9961 aatcggccaa aatcaacgac ctgggtttca ggtgagatag caaagttgac gctgcaatcc
    10021 agcacgcgga tattttccag accggtaatg tgatatttca gattcttcgc ggtagggtct
    10081 tcgttcacgc tgcctgcgcc atcaaactgt acgacgatgt aatcgctgag tttgctctga
    10141 taatcatggg gcggcatggc gcgaacttta acgtaaagcc gcatacgcgc cacaaacgtc
    10201 cgcgatttgt gcataacggt tggatcgccg gagcaaagtt tattttgcca gcccatcgca
    10261 atgatctgct ccggggtgta aatatcaata ttcttactgt cgatgcactg attcgtgtcg
    10321 atgcggcttt tcccaaggct tgcgtcatag tccacgcctt cataggttac gcccaattga
    10381 taatagggat cctgaatacc ggggtaaggg tttacccagg cgtagacgtg ctcgctttca
    10441 aaattaccgt ttgtgttgtt gtcacagtag accggaattt ttatatcgtc agattcccag
    10501 attttatcgc cgggtttgga attggccgga gcggcgaagg gcataatagc ttccgatttt
    10561 tctacagcgc cgtttgcggt accaaaatag cagttcagcg ccagcgccgc ttttagcggc
    10621 aaaaaaatca gacagatggc gagcaacacg cctttggcga atctcatttt ttattctcca
    10681 ccggctgcag gcggcattgc cgctggcagg agagcgtgtt catacgcaat gcgccgtagt
    10741 cgtccatgta gccaagataa aactgcttgc cgctatagct gcccgtgctg gtggtgaccg
    10801 aactgaacgg tgcgaccatc gtgcttttaa agccaggcag cacgcctttc tcatcccgcc
    10861 caagataggc aatagtcaga tagtatccgg tcggggtttt cagggtaagc tggttattct
    10921 gctggctaac ctgtagctgt tgctcaacat gatcgcccat ttttttgcgt aatgcggcgg
    10981 gtcgccagaa tagcttaatg cgactttgga cggcgacttg caggatcgca tggccctcgc
    11041 tgccttccgg gacaggcgga atttcacgca ggttatagta aaagagagat tcgcgatcgg
    11101 tgggtaattt cgcggtggcg gcttgtttca tgatccgaac ctgcgaagtc gcttttggct
    11161 ccagtcgctg gataggcggc agggcgacga gaaattcatc gcttttctct cccttttcat
    11221 tttctatcca tgaataggcg aggtagggga gtttatcgct gcggttttcc acgcgcaggc
    11281 tggttgcttt atctttgcta ttaaagatga tgcgcgtgcg gtcaggctgc actgcgccgt
    11341 ggctgcacat actggcaacc agaagaatca aagggaatcc tttaacaaat gtcatcgtct
    11401 tttccttaat taatgacagg gtaaaatcag gcgcttagtc acatcttcca gatgttccgg
    11461 cagatgaatg gcacacgttt tctggtcgcc ccagtgcacg gtaaattgtt gattttcatc
    11521 gaccccactc agccatgcgt gaccgttttc gccaaccatc ccgacgctga caccgccttc
    11581 agcctggcga acatcggcac cgagcggcgg gaaatgcccg ttagcatcgc tgataatgac
    11641 gttgagatct ttcccggcgc gggacgccag cgatttaaag ccgatggcgc cttcggtcca
    11701 ggtttctttc accacgtttt cggaaacggt aacgccgtca ggcaagtcgt tcatattgac
    11761 cgccaccgtc gtaggctggt agctggaaac aaacggtacc acggcaatac caaagcggtt
    11821 ggtgtagtca atattgccct gaatcggaat atcgcccacg ccgtcggtgc tgaccatcag
    11881 gcgtggttca ttggtggaac tgcggcgatg gaatgccgcg ccatgttgag tcgcggtaaa
    11941 cgagccgctc caactggcac tggcggaagt gtaatcattg gcggcatagg ttccggtcag
    12001 atcccattcc cccgccgaac tgaggtgttg gtaattgccg ctgacctgcg caccgttatc
    12061 cgggcgatcg gattgtattc cagccgacat tccccaactg tttcgttcat ccagggtatc
    12121 gttccacgac atgcggtggg tggtagtatt actgttgttc tgcatgtcat aacccagtcg
    12181 accactttcc ccaatcggta aggagatcga aaagtaaatc tggttgtcgc gatctttatc
    12241 ttcataatgg gtggtgttga acgaggtcga gacagaaata tctttccagt cgccaatgtc
    12301 gacgttaaaa ccgacggtaa tgttggccgt ggtggaggta tccgcattcc accagctctg
    12361 atgcaggatg ttggcgtaga gattaagatt cagcagagta ataggctgac cgaaggagag
    12421 gctgattgtt tgcttttcat cctgcgcgtc ggcgtcattg tatttgtgat caatgtagtt
    12481 agcgtagctg tgaaaatggc gatcggagaa gcgataggct gccaatgaga tggtgctgtt
    12541 ggtcgcatca acctgtttgc tgtagttaaa gcggtagctg taaccctgct catcctgttg
    12601 ggtatcaaaa tggctgtcgg cccaggtcac gtcaaacgac agcgccccca tccagagcat
    12661 attttgccca atacccagcg cgccggaacg atagtcatcg cctgcgagaa gcatgccgcc
    12721 atatagtgaa gtgttggaga gcattcccca ggagacttca tggctaatga aggtttcatc
    12781 ctcggtgtgg tgcgacatag aggaacgcgg gcgaccggca gccagtttat agcgcacctg
    12841 gccctgacgc gtcaggaacg gggtagaggc cgctgaaacc tggaagttat tcacccgtcc
    12901 atcttcttcg ctcactttga catccagtgt accttgcact gactgattca ggtcatcaat
    12961 aataaatggg cctggtggaa cttttttctg gtaaatcacg cgaccagaat ggcttatcgt
    13021 gaccgtggcg ttagtctgtg cgatgccgct gatttgcggc gcgtagccgc gtaactccca
    13081 ggggagcatt cgatcgtcgc tggctaacgc cgcaccggta taggagaaac catcaaaaat
    13141 gttagagcta aaatcggttt cgcccagggt taatcgggaa cctatttgcg gtaacgggcg
    13201 gaacaggtag gtccgtgaaa tcgccccaga ttgttcacgg ttgtcgccgc tatcggactg
    13261 gctaagctga taatcgctgc gcagacgcca tgccccggcg ttgaggcccg tggtgccata
    13321 ggcgttcagg ttgttgctgc tgctgccgct ttgtgggcga taagtgctgg caaacaggtt
    13381 gtaatccatc aggaagccag gaataccgtt attccacgtt gagggtggcg tccagttttc
    13441 tgaatgccac gccagccatg cctgcgggat tgtaatattg agctgttgat ttgcctgatc
    13501 gaaagtaaag atgatttcag ggaagacact aaaatcgaca cattctttta ttgccggtaa
    13561 ttttttacga aagtcagatt taagtccgaa gagttcgatc agcgattcat tgatacaagg
    13621 aataatttta tcgccagatt tttgccagcg aatttgttgc ccattactga ttttattatt
    13681 attaaccgta acactaacaa agtaatcgcc gggagctacg acgccttttt gattgagcaa
    13741 cgaaatatca acgctatccc gaatcgactt atcgagaacg ttaaggttaa attcagctgt
    13801 cagcgcagga ggggttacca ttgccagaca ggaaagaaca gagagtcgat aaatattcac
    13861 ggcatccata cctgaacaat attcgcaaaa ggcggctaat cagccgcctt aaaaaggatt
    13921 attggtaatc cagaacgtaa gttgcgttag cagtaacggc ccccgcggta accgaaactg
    13981 cgttatccat ctgttccatc caggcaaaga agtttaacgt ttgttctgtt gaacttgcca
    14041 ataagtcaat tgttggcgct tctgttccca atgtaacaaa tgattcatct ttgttcatga
    14101 gacgtacgcc aacgccggta gcctcgccag cactgatgtt actaaggagc ggggttgcac
    14161 ctgtcgtttt agccgtgctg tcgaaggtca cgcctacctt tgagaccacg ccacccttgc
    14221 catcatccca gcctgcaata tcacagttga ttaagcgaat attgacaggt tttttctcgc
    14281 tatgatgttc tcgatttatg tgggatgtgg ttacttcacc caggtcaatg ttcatatcaa
    14341 ggtcatcagg gtgaatttca catggtgctt caataacttc acctttaaag tggatttcac
    14401 cagatccttc atctgcagca aaaacaggtg aagcgaatag tagagatatg gccagggcgg
    14461 ccagtgtttt ttgtccttta aacatacatg aatccttcat gggctatgat aaagtatact
    14521 aattattccg acagaatagt ggttttttgt tcatgtaata atattaatgt tgctacatta
    14581 tgtttgagag tttctcaaag ctctcgaatc ataaataaag tgaattaatc ctattacg
//
LOCUS       DBFIFS010000113        14580 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.113, whole genome shotgun sequence.
ACCESSION   DBFIFS010000113 DBFIFS010000000
VERSION     DBFIFS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.113"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(201..626)
                     /gene="arsC"
                     /locus_tag="N7A07_002945"
     CDS             complement(201..626)
                     /gene="arsC"
                     /locus_tag="N7A07_002945"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU2477571.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(639..1928)
                     /gene="arsB"
                     /locus_tag="N7A07_002946"
     CDS             complement(639..1928)
                     /gene="arsB"
                     /locus_tag="N7A07_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU2477572.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1982..2335)
                     /gene="arsR"
                     /locus_tag="N7A07_002947"
     CDS             complement(1982..2335)
                     /gene="arsR"
                     /locus_tag="N7A07_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU2477573.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3079..3162
                     /gene="dinQ"
                     /locus_tag="N7A07_002948"
     CDS             3079..3162
                     /gene="dinQ"
                     /locus_tag="N7A07_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU2477574.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3216..4568)
                     /gene="gorA"
                     /locus_tag="N7A07_002949"
     CDS             complement(3216..4568)
                     /gene="gorA"
                     /locus_tag="N7A07_002949"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU2477575.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4640..5482)
                     /gene="rlmJ"
                     /locus_tag="N7A07_002950"
     CDS             complement(4640..5482)
                     /gene="rlmJ"
                     /locus_tag="N7A07_002950"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU2477576.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5685..7727
                     /gene="prlC"
                     /locus_tag="N7A07_002951"
     CDS             5685..7727
                     /gene="prlC"
                     /locus_tag="N7A07_002951"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU2477577.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7735..8487
                     /gene="rsmJ"
                     /locus_tag="N7A07_002952"
     CDS             7735..8487
                     /gene="rsmJ"
                     /locus_tag="N7A07_002952"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU2477578.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8536..10005)
                     /gene="dtpB"
                     /locus_tag="N7A07_002953"
     CDS             complement(8536..10005)
                     /gene="dtpB"
                     /locus_tag="N7A07_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU2477579.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10171..10224
                     /locus_tag="N7A07_002954"
     CDS             10171..10224
                     /locus_tag="N7A07_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477580.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10202..10255
                     /locus_tag="N7A07_002955"
     CDS             10202..10255
                     /locus_tag="N7A07_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477581.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10322..10756)
                     /gene="uspA"
                     /locus_tag="N7A07_002956"
     CDS             complement(10322..10756)
                     /gene="uspA"
                     /locus_tag="N7A07_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU2477582.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11147..11482
                     /gene="uspB"
                     /locus_tag="N7A07_002957"
     CDS             11147..11482
                     /gene="uspB"
                     /locus_tag="N7A07_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU2477583.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11553..13052)
                     /gene="pitA"
                     /locus_tag="N7A07_002958"
     CDS             complement(11553..13052)
                     /gene="pitA"
                     /locus_tag="N7A07_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU2477584.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13284..14486
                     /gene="yhiN"
                     /locus_tag="N7A07_002959"
     CDS             13284..14486
                     /gene="yhiN"
                     /locus_tag="N7A07_002959"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU2477585.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
BASE COUNT         3658 a         3731 c         3862 g         3329 t
ORIGIN      
        1 gacgcgataa ttggaatctc acgcagggag taatgtgtaa atagcaaaat cgcctgatgc
       61 gctacgcctg tcaggcctac aaataacata caatttattg aattttcatg actttgtaga
      121 tcggataaga cgcgttagcg tcgcatccga cataaataaa gcgcactttt ctaacaacct
      181 gtggggggga tatagccgct ttatttcagg cgcttacccg cttcatcaac cactttctcg
      241 ccatcttcct tggtgaatgc gcctttttgc gcatctggca gaatttccag caccacttct
      301 gaagggcggc acaggcgagt tcccagcgtc gtcaccacaa tcgggcgatt aatcagaatc
      361 gggtgctgaa gcataaagtc gattaaccga tcgtcagtaa atttatcttc cgcaaggccc
      421 agctcctcat acggttcgac gtttttacgc agcagcgcgc gtacggaaat ccccatatcg
      481 gcaatgagtt tgaccagttc atcgcgcgtt ggcggagttt ccagataatg gataatggtc
      541 ggttctgtgc cgctgttgcg gatcatctcc agcgtattac gcgacgtgcc gcaggccggg
      601 ttgtgataaa tggtaatgtt gctcatatca gtatctcatt acaaagtgaa agagagacgt
      661 agcgccagcg cagccagcgt cacaaacagc acaggcaggg tcatgataat ccctgtacgg
      721 aagtaatatc cccagctgat agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc
      781 gttgccaggc taccaatcgg ggtgattttc ggccccaaat cgcagccaat cacgttggca
      841 taaaccatcg cttctttgat aacgccagat gccgtgctgc catcaatgga caacgcgcca
      901 accagtaccg tcggcatatt gttcataata gaagagagga aggcggtgag gaatccggtg
      961 ccgagcgtcg cggcccacag gccgttatcc gccagcacgt tgagtacgcc agaaagatat
     1021 tccgttaatc cggcattgcg caggccataa accaccagat acatgccgag cgagaagatg
     1081 acaatctgcc agggcgcacc gcgcagcact ttacccgtat taatcgcatg accgcgttta
     1141 gcgacgacaa ataatatcaa cgcgcccact gccgcaatgg cgctgaccgg aatgccgagc
     1201 ggttccagga cgaaaaatcc caccagcaga agcagtaaaa caacccagcc agttttgaac
     1261 gtagcaggat ctttaatcgc ttctgcggga gatttcagca gcgccatgtc gtagttctgc
     1321 ggaatatctt tgcggaaata gagatgcaac atcaccagcg tggcaacaat cgcggcgata
     1381 tccaccggca ccatcaccga ggcatattcg cgaaagccga ggccaaagaa atctgcggaa
     1441 acgatattca ccaggttgga gacaataagc ggcaggctgg cggtatcggc aatgaatccg
     1501 gccgccatca caaacgccag cgtagtgcct ttactgaacc ctaaagccag cagcatggcg
     1561 atgacaatcg gtgtcaaaat aagcgccgcg ccatcattgg caaacagggc ggcaacggca
     1621 gcaccgagca ggacaatcca ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa
     1681 acgtgcagtg ccgcccattc aaaaaagccg gactcatcca gcagcaggct gatgataata
     1741 acggcaataa acgcagccgt cgcgttccag acgatattcc acaccaccgg aatatcgccc
     1801 ggatgtacta cgcccgtaac taacgccagt actgcgccga gcgttgcact ccagccgatg
     1861 cctaaacctt tcggctgcca gataaccaat acaatggtca ggacaaagat agcgcctgcc
     1921 agtaacataa tgcctcccgg aaaaacacat gtgcaaatcc atatgtgttt agataaattt
     1981 tttaactaca agtgttctta ctgtctacgg aacaattttg tctggccagg ttgcggacaa
     2041 tcacctgaac cttttcctgt tcgcatcgcc aggcctgctc aataattttc gcggcccatg
     2101 atggaatatg cggtgataag cggtaatgaa cccacttacc ctgcttgcga tccagcaaca
     2161 aaccgctttc acgaagaagc gccagatggc gggagatctt gggctgcgac tggtcgagag
     2221 cagtgcagag atcacagacg cataactctc ccagctcgct gagcagtaaa acgatgccca
     2281 gacgggtttc atcggcaaga attttgaaca gttggatggg taacagaaat gacatattgc
     2341 gctcctgatt gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa acatatatgg
     2401 tttaacgaat gtgtatgtgc agaggaaggt aataggtgtg aattaagcgt tggctattca
     2461 tttgaaagga ggtctgaatc aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt
     2521 ccagcccaga gttggtggct ctggctggag tgagaatgcc cacatctaac ggttcaatac
     2581 tattagccag tggctaacat tgcaagcgtt agcgatttct tcagctggag aagggacagt
     2641 ttgctataca tgttggtgaa agaggattta tatactttca tcgggtcgct gccgtaccag
     2701 gctttgtccg aatccggatg taaaaaaacc gacttcgcgt cggttttttt actcttcagc
     2761 cctgagttgg tggctctggc tggagtgaga atgccatcat ctaacgcatc aatactaata
     2821 ccatactgaa actattgcaa ggacatgctg gttttataac ctgaatatac tgtatgatta
     2881 tccagttagc tctgaggcat tttcactctg gcaatgcgca taaacgcttt caaagtcctg
     2941 ttcagtggta cgggtggtgc cgttaactga tgctctggcc ggagtgagaa taccattatc
     3001 taacaatagg gcatgcgccg tgacaggcga tggatgagta agcggatgca ttctcactcc
     3061 atcgtatgga gaacacgggt gattgataaa gcaatcatcg ttcttggggc gttaattgcg
     3121 ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa acgtaattaa
     3181 gggcaaagag cacaccactc tttgcccttt aacatttaac gcatggtcac gaactcttct
     3241 gccgccgttg ggtgaatggc gacggtatta tcgaagtctt ttttggttgc gcccatcttc
     3301 agtgccaccg cgaagccctg caacatttcg tccataccaa aaccaatgcc gtgaataccg
     3361 acaatcttct cttctggccc aacgcacacc agcttcatgc ggcacggctg gcggtgagtg
     3421 gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg atcgtcgccg
     3481 tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg atggctgaag
     3541 accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt aaacaggcgt
     3601 tcagagagac ggcgacccgc tgcaactgcc accggtgtca gttctactgc acccgtgtta
     3661 tcgcccaccg cgtaaatacc ttcaacgttg gtgttttgat atttatcgac gacgatatag
     3721 cctttttcgt tggttttaac gccagcggct tcgaggttga tgttgtcatt ggcaggctcg
     3781 cggccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc cagctccagc
     3841 gtcaggctac catcggcatt tttcactacc gctttcggga tggcgttggt gtgcaactgc
     3901 gggccttcgg cgttcatcac ttcgaccagc gtttcagaga tcatcgggtc gaagctgcgc
     3961 agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt aatcacgcca
     4021 gccagctcaa cggcgatgta acccgcgcca acaaccgcca cacgctctgg caaagcagga
     4081 agggcaaaga aaccatcaga atcaataccg tattccacgc ccggaatatc cgggtggctc
     4141 ggacgaccgc cggtggcgat cagaatatga tcggctgtga tggtttcacc gttaacttcc
     4201 agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt atttttaccg
     4261 agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct ggcgatcaac
     4321 gtttcccagt tgaatttatt gatagtggta tcaaaaccat aatccgggcc gtacatatgg
     4381 atcgcttcac ggatttgcgc cgcgtgccac atcactttct tcggtacgca gccgacgttc
     4441 acgcaggtgc cgcccagctc tttggcttca atcagcgcgc atttctgacc gtacatagcc
     4501 gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta atcatagtgt
     4561 ttagtcatga cagagtgtcc ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag
     4621 gtgccagcaa tggctgcaat tactctggca cgatccagct tacggtggcg tgcccggtgc
     4681 cagctggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgc tgttccagtt
     4741 tccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga tcgctgtctg
     4801 gcagtaccgc cagttcaatt tgcagaattt tgcgaatgcc ggtcgcttcc aggtcgtgga
     4861 tcatgcgctt aatttgctgg cgcagcacca ccggatacca caatgcgtaa gtgccagtgg
     4921 cgaaacgttt gtaaccttct gctatcccgc tgactaccgc ctgatagtcg gttttcattt
     4981 catacggcgg atcgataagg attaaaccac ggcgagaaac cggcggcagt ttggccttaa
     5041 gctgctggaa accgtcggat ttttcgacgc gcgcacggct gtctttctga aattcagaac
     5101 gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtcc tgttcacgca
     5161 gtaactgacg ggcaatcaac ggtgaaccgg ggtagtaacg caactgaccg ctacggttga
     5221 agtgttttac cacattgatg tacggctcca gctccgcagg caaatcgtcc tgctgccaga
     5281 tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgctg cctaactgat
     5341 aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc tctttcagcg
     5401 actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa
     5461 agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat cggcccgtat
     5521 tcggggcgca caaaagccta tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc
     5581 gtaacgtttc tcattgaaat tcactacact taaccccatg ctacaaacat tatgtaaagc
     5641 gcctgttgag cgcttcctta acctctttaa ccaggactgc gctaatgacg aatccgttac
     5701 tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc gttccagccg
     5761 tgactaaggc attgaatgac tgccgcgaaa atgtggagcg cgtagtagcg caaggagcac
     5821 cgtacacctg ggaaaatctc tgccagccgt tggcggaagt ggacgatgtg ttggggcgta
     5881 ttttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaattg cgcgaagcgt
     5941 acgaacaaac tctgccgctg ctgtcggaat acagcacctg ggtagggcaa catgaagggc
     6001 tgtataaagc gtaccgcgac ctgcgcgatg gcgatcatta cgccacgctg aacacggctc
     6061 agaaaaaagc ggttgataac gcactgcgcg attttgaact ctcaggcatc ggtctgccga
     6121 aagagaaaca acagcgttac ggcgaaattg ccactcgtct ttctgaactg ggcaaccagt
     6181 acagcaacaa cgtccttgat gcgacgatgg gctggactaa actcgttacc gacgaagcgg
     6241 aactggcggg gatgccagaa agcgcgctgg ctgcggcaaa agcccaggcc gaagcgaaag
     6301 agctggaagg ctacctgttg acgctggata tcccaagcta cttgccggta atgacctact
     6361 gcgacaacca ggtcttgcgt gaagagatgt atcgtgctta cagcacccgc gcttcagatc
     6421 aaggtccaaa cgccggtaaa tgggataaca gtaaggtgat ggaagagatc ctcgcgctgc
     6481 gtcacgaact ggcgcaactg ctgggctttg aaaactacgc gtttaaatca ctggctacca
     6541 aaatggcgga aaacccgcag caggtgcttg atttcttaac cgatctggca aaacgcgcgc
     6601 gtccgcaggg cgaaaaagag ctggcgcaac tgcgtgcctt cgccaaagcc gaatttggcg
     6661 tggatgagtt gcaaccgtgg gatatcgcat actacagcga aaaacaaaaa cagcacctct
     6721 acagcatcag cgacgaacaa ctgcgtccgt acttcccgga aaacaaagcg gttaacggct
     6781 tgtttgaagt ggtaaaacgt atttacggca tcaccgctaa agagcgtaaa gatgttgatg
     6841 tctggcaccc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca
     6901 gtttctacct cgacctgtat gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact
     6961 gcgtaggcca gatgcgtaaa gccgatggtt cgctgcaaaa accggtcgcg tatctgacct
     7021 gtaacttcaa ccgcccggtg aatggtaaac cggcgctgtt cactcacgac gaagtgatca
     7081 ctctgttcca cgagttcggt cacggcctgc accatatgct gacccgcatc gaaaccgccg
     7141 gtgtttccgg tatcagcggt gtgccgtggg atgcggtcga actgccgagt cagtttatgg
     7201 aaaactggtg ctgggagccg gaggcgctgg cgtttatctc tggtcactac gaaaccggcg
     7261 aaccgctgcc gaaagagttg ctggataaaa tgctggcggc gaagaactac caggcggcgc
     7321 tgtttattct gcgccagctg gagttcggcc tgttcgattt ccgcctccat gccgagttcc
     7381 gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg
     7441 tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg
     7501 gttatgccgc aggttactac agctacctgt gggctgacgt actggcggca gatgctttct
     7561 cgcgctttga ggaagagggc attttcaacc gtgaaaccgg gcagtcgttc ctcgacaaca
     7621 ttctgagccg tggcggttca gaagagccga tggatctgtt caaacgcttc cgtggtcgtg
     7681 aaccgcagct ggatgcgatg ctggagcatt acggcattaa gggctgatca ttcagtgaaa
     7741 atctgcttaa ttgatgaaac aggcaccgga gacggtgcct tatctgttct ggcggcccgc
     7801 tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacgcc ggaacatctg
     7861 gaattgcgca agcgtgatga gccaaaactt ggcggcatct ttgttgattt tgttggcgga
     7921 gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg aggcggtggc gaaagcggtg
     7981 ggcattaaag gcgattattt gccggatgtg gtggacgcta ccgcagggct ggggcgcgat
     8041 gcctttgtgc tggcttcggt cggctgccgc gtgcggatgc tggagcgtaa tccagtcgtt
     8101 gccgcgctac tcgacgacgg cctggcgcgt ggatatgcgg atgcggaaat cggcggctgg
     8161 ttgcaggagc gtttgcagtt aattcacgcc tccagcctga cggcgctgac cgatattacc
     8221 ccgcgtccgc aggtggtcta tctcgacccg atgttcccgc ataagcagaa aagcgcgctg
     8281 gtgaaaaaag agatgcgcgt ctttcagtcg ctggtgggac cggatcttga tgccgatgga
     8341 ttactggagc ctgcacgctt gctggcgacc aaacgcgtgg tggtaaagcg cccggactac
     8401 gcgccaccgc tagcgaatgt cgccacgcca aacgcggttg tgactaaagg gcatcgcttt
     8461 gatatctatg caggtacgcc agtgtaaaag taaagcaggg tagcgataac gctaccctgc
     8521 tttctgcaag aataattaat ggctttccgg cgtcgcaatc atgcgtttca gccacggcac
     8581 catcaacagc atcactactg caacgcccag cgtgaccaga ccaatcttac caaacacgtt
     8641 ggtatagaca ggcaacgtct caagcggatc ggtaatgttg tctggtactg cggtaaatgt
     8701 tgccacatag ccgcccagca agaacgcggc agcctgtgtc aggaaccaca tcccgagaat
     8761 aaagcccatc aaatgctgcg gcaccagagc agcaatcatc gccaggccaa gggcgctaat
     8821 aaacagttca cctaagctct ggaataagta caccagcacg ataaaccatg gcgatgtcag
     8881 cccttgtgca tccgcaaacc acattcccgc agctgccgcc gtcaaaaagc ccagtgagca
     8941 cataaacatg ccgagagtaa atttcatcgg catcgagagg tctttgcctt tgttacccag
     9001 atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg ggttaagcgc
     9061 ctggaagctg accgggttga tggaaaaacc gagaatttca tgatgcacgt tgttgatggc
     9121 aaagaagttc agcgatgttg gcatctgggc gtagagaatg taaaacacca ccgcttcgag
     9181 catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct tgaatgcctg
     9241 acgaaagaag atgatggtga cgacgatgga gagaacaatc agcaccagat tggcgacttc
     9301 tacgttgtgc atcagccagg cgcagacgaa gatcatcacc acgctgccaa gtaatacgta
     9361 caacagtttg ctgaagctca ttggccggaa gtcgggctca gaaccaatgt ctttcaccat
     9421 tccacgacag gcgatgtaaa ccagtaatgc gataattaac cccgcaccgc acaggttgta
     9481 ggtgactgaa tagccgaatt tatcagcgat cacaggggcc agcgataacg ctatcaacga
     9541 gccgatattg atcgacatat agaacagggt gaatgtgcca tcaaggcgcg gatctttcgg
     9601 cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt taccgacagc
     9661 gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacatgc cggtcatgaa
     9721 gtagccaatc gccagcacaa gtgctccaag aacgatggtg cgtttggtcc ccagcaggtg
     9781 gtcgccgaca tagccgccaa tggaaatgag gccatagacc agtgcagcaa aagcaccaaa
     9841 agtgacaaaa gcctgctctt gcgagaatcc aagctgttta acgaagaaaa ccgccagtac
     9901 gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa
     9961 tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta ttaaccttcc
    10021 aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt
    10081 cagagattct taatattgat gcggtgaata gattaataca ctattacaac agaaaataac
    10141 cagctgatca cactaatgat ggaaaatatg atggtgttaa atattgtatt ggctggggat
    10201 tttgattgcg aacgcagacc gtaggccaga taaggcgttt acgctgatca ggtaacaagg
    10261 cgcgacaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tggagggaag
    10321 attattcttc ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg gtgttgatca
    10381 gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca caaaccacta
    10441 gatccatatc gtatttcttg attgcatcga ccagaacctg gcccaggtcg ccgctgccgc
    10501 tcagggtttc agtgattggg tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg
    10561 tctcttcaga gatgcgtttc tgcatatcac caagattcac atcaataagc ccggtatata
    10621 ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtat gggcgagcca
    10681 tagagactgc tttctctacc agaactttgc tttccgggga gagatcgacc gcgatgagaa
    10741 tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc
    10801 gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc
    10861 cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc
    10921 cgccttgctt acccgttaga taaacaaatt ttcgacatga atatattgat agtggttaac
    10981 cttctggaaa aaaaacaacc tgatctccta cactatctat agagccgctc ggatgttggt
    11041 caccacaaaa gcggtttctc caggacctgg tgttaactga ttggcagcgt atcgggagcg
    11101 gtagccccgg ggtggaactc cgcgggcagg tcgccgggga ggaggtatga taagcaccgt
    11161 cgcattattt tgggctttat gtgtcgtttg cattgttaac atggcgcgct atttctcatc
    11221 actacgcgcg ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc aatatgttga
    11281 tggagggggc ttttttacct cacatggcca acccaacaaa caggtgcgtc tcgtttggta
    11341 tatctatgcc caacgttatc gcgatcatca cgatgatgag tttattcgcc gctgtgagcg
    11401 ggtgcgtcgg cagtttattc tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat
    11461 tgccttgatg atttggcatt aagcacttca gcaataataa cgcataaaaa aagcgggcca
    11521 gtaaactgac ccgctcgttt tggtgcgtac tattacagga actgcaagga gagccagtaa
    11581 agcccgccgg aaagcagtac cgcagccgga agggtaaaca cccaggccat cagaatgctg
    11641 gtcacggttt tacgctgtaa gccgccacca tctaccacca tcgtccccgc gacagaagag
    11701 gagagtacgt gagtagtgga aaccggcatc ccggtataac tcgccaggcc gatagacact
    11761 gccgcagtca tctgggcaga catcccctga gcgtaggtca tgcctttctt accgattttc
    11821 tcaccgatag tcgttgccac acgacgccag ccaatcatcg taccgatacc taacgccagc
    11881 gcgaccgcca tgatgatcca caccggtgca tactcgatgg tgctaagcat gtcggacttc
    11941 agtttcttca acaggcgctg atcgtcagca ctcacgccag gcatcttcac cactttgtcg
    12001 atagtgtcag aaacgcacag cataatgcgg cgcatctggc tacgttgatc aagcgacagc
    12061 ttgtcgtagc tttccacatc ggtggtcaac atacctttca ggcggttgag cgcgttaatg
    12121 gtattcgacg gatggcagtg gaactccgca ggttgcgttg cgccagcttc cggagccggt
    12181 actaactgat cagcaccagt agcttgtttc agcagcgcag gatgctgctc aaagtaagct
    12241 tcgacgttgt tgatggcatc acgggtacgg gtgatttcgt agccagtggc attcatgttc
    12301 accacgaagc ctgctggtgc gacaccaatc aataccaaca taaccagacc aatgcctttc
    12361 tgaccatcgt ttgcgccgtg cgaaaacgcc acgccgatag cggaaaggat cagcgcgata
    12421 cgcgtccaga acggcggctt tttcttgccg tctttctttt cacgctccgc tggggtcagg
    12481 tggatacggg cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg
    12541 ccagcaaaca ccaggccgac aataggggaa acgatcagag aaccgaaaat acttaatact
    12601 ttcgggatat tgagtgcatc caccactgac gtcccggtca tcaacgcatt ggttaaacca
    12661 atcccgatga tcgcgccaat cagcgtatga gagctggatg caggcaaacc aaagtaccag
    12721 gtacccaggt tccagataat cgccgccagc aacatagaga acaccatggc aaggccatga
    12781 gacgatccca tattaagcag cagatccgtc ggcagcatat gcacaatggc ataggcaaca
    12841 ctcagaccac ccagcaaaac acccaaaaag ttgaacaccg ctgccataac cacggcgagc
    12901 tgagaacgca tcgcgcgggt atagataacg gttgccacgg cgttggctgt gtcatggaaa
    12961 ccattgatgg cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa cagcccggta
    13021 tgcaaatcca ggccagcaaa caaatgtagc ataggacgtt acgccatttt gaggacatga
    13081 acgcggcgca ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag actttttttg
    13141 atttgcctcc tgtatggatt tcactcaaaa aataattatc ttatataatt caggcaaata
    13201 cttcctttta gtaatattga tgctggtgcg accactgagg aatctttaca attcacgccc
    13261 gttttttcta agaggagcgc aacgtggaaa ggtttgatgc cattattata ggcgctggtg
    13321 cggcgggtat gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg
    13381 ataatggtaa aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta
    13441 ccaaccttta tgtcgaaccg ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg
    13501 cactcgcgcg ttttacccag tgggatttca ttgatctggt caataaacac ggcatcgcct
    13561 ggcacgagaa aacgttaggg caactcttct gcgatgactc cgcgcagcag attgtcgaca
    13621 tgctggtgga tgagtgcgag aagggcaatg tgaccgtcag attgcgtagc gaagtgctga
    13681 gtgtggcgaa ggatgataca ggcttcacgc ttgaactgaa cggcatgact gtcggttgtg
    13741 aaaagctggt catcgcgacc ggcgggctgt caatgccggg cctgggcgcg tcgccgtttg
    13801 gttataagat tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc
    13861 cattcactct gcataaaccg ttgctcgaag agttacaggt gctggcgggc gtggcggtgc
    13921 cttccgtgat taccgctgaa aacggcaccg ttttccgtga gaacttactc ttcacccatc
    13981 gcggcttgtc tggaccggcg gtgttgcaaa tttccagcta ctggcaaccg ggggaatttg
    14041 tcagtatcaa tctgctaccg gatgtggacc tcgaaacctt cctgaatgag cagcgtaacg
    14101 cacatccgaa tcagagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg
    14161 aacgcttaca gcaactcggg caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg
    14221 accaacaggc actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag
    14281 gctatcgcac tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga
    14341 cgatggaagc gcgcaaagtg cctgggctgt actttatcgg cgaagtgatg gacgttaccg
    14401 gctggctggg gggctataac ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg
    14461 atttgattgc agcgaagtcg tgctgagcta tttttcctaa agatgaaaat tattagagtc
    14521 gcattaaaaa tgagctaatt ttgatagtgg ctatcttgtg attattttct aatgagcccg
//
LOCUS       DBFIFS010000114        14475 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.114, whole genome shotgun sequence.
ACCESSION   DBFIFS010000114 DBFIFS010000000
VERSION     DBFIFS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.114"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..171
                     /locus_tag="N7A07_002960"
     CDS             <1..171
                     /locus_tag="N7A07_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477586.1"
                     /translation="LVTREIQDEIKSKIQDKIDELSKNLFNTMTETIENNFDDIFRQQ
                     SENMSNYYEFVD"
     gene            complement(349..864)
                     /gene="pgpA"
                     /locus_tag="N7A07_002961"
     CDS             complement(349..864)
                     /gene="pgpA"
                     /locus_tag="N7A07_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU2477587.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(842..1819)
                     /gene="thiL"
                     /locus_tag="N7A07_002962"
     CDS             complement(842..1819)
                     /gene="thiL"
                     /locus_tag="N7A07_002962"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU2477588.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1897..2316)
                     /gene="nusB"
                     /locus_tag="N7A07_002963"
     CDS             complement(1897..2316)
                     /gene="nusB"
                     /locus_tag="N7A07_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU2477589.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2336..2806)
                     /gene="ribE"
                     /locus_tag="N7A07_002964"
     CDS             complement(2336..2806)
                     /gene="ribE"
                     /locus_tag="N7A07_002964"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU2477590.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2895..3998)
                     /gene="ribD"
                     /locus_tag="N7A07_002965"
     CDS             complement(2895..3998)
                     /gene="ribD"
                     /locus_tag="N7A07_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU2477591.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4002..4451)
                     /gene="nrdR"
                     /locus_tag="N7A07_002966"
     CDS             complement(4002..4451)
                     /gene="nrdR"
                     /locus_tag="N7A07_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU2477592.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4602..5141
                     /gene="yajI"
                     /locus_tag="N7A07_002967"
     CDS             4602..5141
                     /gene="yajI"
                     /locus_tag="N7A07_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU2477593.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5440..6324
                     /gene="tsx"
                     /locus_tag="N7A07_002968"
     CDS             5440..6324
                     /gene="tsx"
                     /locus_tag="N7A07_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU2477594.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6501..6848)
                     /gene="yajD"
                     /locus_tag="N7A07_002969"
     CDS             complement(6501..6848)
                     /gene="yajD"
                     /locus_tag="N7A07_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU2477595.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6936..7217)
                     /locus_tag="N7A07_002970"
     CDS             complement(6936..7217)
                     /locus_tag="N7A07_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2477596.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7210..7452)
                     /locus_tag="N7A07_002971"
     CDS             complement(7210..7452)
                     /locus_tag="N7A07_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU2477597.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7604..8575)
                     /gene="secF"
                     /locus_tag="N7A07_002972"
     CDS             complement(7604..8575)
                     /gene="secF"
                     /locus_tag="N7A07_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU2477598.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8586..10433)
                     /gene="secD"
                     /locus_tag="N7A07_002973"
     CDS             complement(8586..10433)
                     /gene="secD"
                     /locus_tag="N7A07_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU2477599.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10461..10793)
                     /gene="yajC"
                     /locus_tag="N7A07_002974"
     CDS             complement(10461..10793)
                     /gene="yajC"
                     /locus_tag="N7A07_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU2477600.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10816..11943)
                     /gene="tgt"
                     /locus_tag="N7A07_002975"
     CDS             complement(10816..11943)
                     /gene="tgt"
                     /locus_tag="N7A07_002975"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU2477601.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11998..13068)
                     /gene="queA"
                     /locus_tag="N7A07_002976"
     CDS             complement(11998..13068)
                     /gene="queA"
                     /locus_tag="N7A07_002976"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU2477602.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13161..13742
                     /gene="acpH"
                     /locus_tag="N7A07_002977"
     CDS             13161..13742
                     /gene="acpH"
                     /locus_tag="N7A07_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU2477603.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13763..>14475)
                     /locus_tag="N7A07_002978"
     CDS             complement(13763..>14475)
                     /locus_tag="N7A07_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477604.1"
                     /translation="DKLHKLYKTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAK
                     KTAEIIAKQNAKSIEEKNIEKNKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRP
                     FFTSDHIYCLFDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGG
                     GLHRHISVKSEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSS
                     ECFSYRCYAAPLDANAKK"
BASE COUNT         3610 a         3862 c         3449 g         3554 t
ORIGIN      
        1 ttggttaccc gtgaaattca agatgaaata aaatcaaaga ttcaagacaa aatagatgaa
       61 ctttctaaaa atttattcaa cactatgacc gaaaccattg agaataattt tgatgatatt
      121 tttcgtcaac agtcagagaa tatgagcaat tattatgagt tcgtcgactg acgatatttc
      181 agtgtcattg aattatgagt gacaaagata caccttgaaa tatcgccgca tagtatattc
      241 ggcgatgtcg ggttttactt taacagagtg ccccaaaatt ggggcactgt tcatttataa
      301 atcaaaccgg gtggtctgga atcgcctaca cctcccggtc aatggcattt aaagaatacc
      361 cagcggccag tgatgaccga taaagtacag gatgcctgcg gaaatcaccc cggcgacaat
      421 atcatcgatc atgatcccca tgccgccatg tacattgcga tcaaaccagc ggatcggcca
      481 cggcttccac atatccagaa tacggaaaat cacaaacccg gcggcgaccc actgccagtc
      541 attggtcggc agcgccatca gggtgatcca catgccaata aactcatccc atacgatgct
      601 accgtgatcg tgcaccccca tgtctttcgc cgtttgatga caaagataga cgccgataca
      661 gatccccaac atcaccacca gcgagtagag ctgccagggc aaaaaggtca tcagatacca
      721 gaacggaatc gccgccagcg atcccatcgt gccaggaacg attgggctta atccacttcc
      781 gaatccgaca gcaagcagat gccacggatt actcatcttc aggcgacttt tcgcgacatc
      841 tttatggcgt ggcaaaatgg tcatatcctt tccagtctaa cgtgacaggt tcgccgtcac
      901 gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta aacggtacgc
      961 ccagatgacc gagagccaca tccagcgcgc cacggttcag ttccggcacc gtgaaacaca
     1021 actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt acaacatggc
     1081 gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg
     1141 ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata gctgaatttg
     1201 ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga tggacggaga tgacgtttga
     1261 tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc
     1321 cggcggcgct atcgcccggt gtaccggtca catagatcca gtcacccggt ttcgccccag
     1381 agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc attgataatg
     1441 gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga agatcaaaca
     1501 aactgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt aatgccagcg
     1561 tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc gccagtgctt
     1621 tataagccag atcagcagga tcgatatcag ggaggaaatg gttgcccgcc accagcgtat
     1681 cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagagcgcaa tcgtcgccga
     1741 tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg gcaatcaggg
     1801 agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc
     1861 gcggaacagt cttccgtgaa tctaccggcc tggatattac tttttgttag ggcgaatcac
     1921 aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg cgccgaacga
     1981 tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcgc tacgtttaga
     2041 cagttcgtac agcgcaatgc gcagtactgc tttttctacc tgtcccagtt cttccagcag
     2101 gcgggacagg tatggcttca tcagtccgtc gaggtatgcg gtattagtcg ccaccccggc
     2161 cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag ccaggaactg
     2221 gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg cctggacggc
     2281 acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac taatttcagg
     2341 ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct tctgcacctt
     2401 tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca gtggtcagaa
     2461 ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac
     2521 caccagcgac atattcaaaa tgggcagtgc caccacgaat aaccgtaccc agcgcaatca
     2581 ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc tcataggcac
     2641 caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt ttcagtgcat
     2701 caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg
     2761 cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc ttccttcggg
     2821 ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg ctgcttccct
     2881 ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc tacatgacgt
     2941 atctctttga atttaaattg gggggcgtca gctaatttct caagccctgg cagtgtgcat
     3001 aatccacggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag ctcatcgact
     3061 aaccccgcct gcagcaatgc gccagcgagc gttggccccg cttccaccca gatgctgtta
     3121 atctgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc tttatgctct
     3181 ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg cgtacgcgcg
     3241 aaccaggttt cgccgggctg ctgcacaatg tgatgttccg gcgtcacgcg attttggcta
     3301 tcaatcacaa tacgtaccgg ctggcggaga tgatgttgcg ggtagagcgc ctgagtttgt
     3361 acatccagtt cagaccaacg caccgttaag gcaggatcat ccgccagcac cgtagcgctg
     3421 ctggttaaaa tggcatgact ttgtgcgcgc agtcgttgta cgtcgcgccg cgcctgaggc
     3481 gaagtgatcc actggctttc gccgctcgcc atcgccgtgc ggccatcaag cgatgcgcca
     3541 agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc tttattcaat
     3601 tgctcggctt cactcatcat caggccgtgg ctgacgtcaa tgccagcctg ttgcagacgg
     3661 taaagtccac gcccagcgac ctgcgggttc gggtcctgca tcgcagcgac cacgcgcgct
     3721 acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac gaccatgatg gctacagggt
     3781 tcgagtgtga cataggcggt cgcacctttg gctttttcac ccgccatacg caacgcgtgt
     3841 acttcggcat gtggttcacc cgctcgttgg tggtaacctt caccgacaat ttcgccatct
     3901 ttgacaatga cgcacccgac attcgggttg gggtgggtgg taaaacgtcc tcgttgcgcc
     3961 agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg
     4021 atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc aaaacggata
     4081 taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat catcttgctc
     4141 ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat tgccatttcg
     4201 acgtcatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct acgtaatttt
     4261 tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat aaccagctcc
     4321 gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg gcggcgtacg
     4381 gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc gaaacagaat
     4441 gggcaatgca tacggcgtcc tgaccaggtg gttaacggaa atctatttta ccctgaactg
     4501 acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg ctgcttttac
     4561 cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatatt aacgtttttc
     4621 gactgatcct gctgggaagc ctattcagcc ttagcgcctg tgtgcagcaa agtgaagtgc
     4681 gacagatgaa acacagcgtc agcacgctga accaggagat gacgcaactc aatcaagaaa
     4741 cagtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc
     4801 tgcctggagc gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc
     4861 tggtgaatat tacgcctgat acagatggca ccaccctgac actacgtatt cagggtgagt
     4921 cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa
     4981 tagacaactt tcaggaaatc aatgtacaga atcaattgat taatgcccct gccagcgtcc
     5041 tcgcccccag cgatgttgat attccgttac agttaaaggg gatttctgtg gatcagttag
     5101 gctttgtacg tatccacgac attcaacctg ttatgcagta aacttatagc ggggcgattt
     5161 tgtgcgtccc gcaacatcct tccccatcat tttgttactc cgcttacata acccagattg
     5221 atagtaaaag tttgcaacaa gggcgaaagt cagtacaatc cccgcccgaa tgtgtgtaaa
     5281 cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgagc
     5341 aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcgcctt
     5401 tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca
     5461 gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa
     5521 ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc
     5581 cgtttcggac cgcagatccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa
     5641 gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat
     5701 gcaaaaggta tctggaacca cggttctcct ctgtttatgg aaatcgaacc acgtttctcc
     5761 atcgacaagc tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg
     5821 aacaactaca tttacgacat gggtcgtaat aaagatggtc gccagagcac ctggtacatg
     5881 ggtctgggta ccgatatcga cactggcctg ccgatgagcc tgtccatgaa cgtctatgcg
     5941 aaataccagt ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc
     6001 aaaattaaat actttgtgcc gattaccgat ctgtggggcg gtcagctgag ctacatcggc
     6061 ttcaccaact tcgactgggg ttccgattta ggggatgaca gcggtaacgc aatcaacggt
     6121 attaagaccc gtactaataa ctctatcgct tccagccata ttctggctct gaactacgat
     6181 cactggcact actctgtcgt agctcgttac tggcacgacg gtggtcagtg gaacgacgat
     6241 gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttac
     6301 ctggtagtag gttacaactt ctgattatga aaatgccggg atttattccc ggcatttcag
     6361 attgttaaca acgtacgctt tgttcatgcc ggatgcggcg taaacgcttt gtccggccca
     6421 caaaagcacg taaattcaat atattgctga aataacgtag gcctgataag cgtagcgcat
     6481 caggcaattt tacgttttaa tcacttcttc ttgttcatca tcgctttcag gtcagcgaat
     6541 gggttgtact tcgcttcacc gacatctttc tgcgcatctt cccctgcgat aacggtcgta
     6601 ccatactgat ccgcttcggt atatttcgaa tgctcatgat cgtggcaata gagacaaagc
     6661 aattcccagt tactgccatc ttccgggtta ttggtatggt cgtgatcaat gtggtgaacg
     6721 gtaagttcac gcaggttgga ataaacaaac tcgcgggaac agcgaccgca gacccacgga
     6781 tagattttta atgctttttc gcgatagccg ctttctaacc gcgcatagtt ttttgggatg
     6841 atagccatga aaagttacct ggatgaaatg tagggttacg cgcagtttcc caagatgcgg
     6901 ggattttcgc aatcctgagt caagaaattc tcacattacc agtgacggcg aggttccatc
     6961 aactgatgaa gaatgcgcgc aatgagaatg tcatcgactc ttataacgta ataaacagtg
     7021 tgaaacctaa atgcgatctt ctttacctct ggaatcgcat catagtcttg ccctatctcg
     7081 ggcatttgag agagtgtatc cagaaaagtg ccgagagagt cggtatagcg atccgcctgc
     7141 tcatcaccaa actttcccag cgtgtagtca tagatccctc tgaaatcttc aacagctttt
     7201 ccgctaagct tatacacgat gtttttgctt cctttctgct gcgatatcac gaagcgtcat
     7261 cgaactttca ccgctctcaa gaccttcaat aacagcattt ctgacagctt catccctgga
     7321 ttcttgttgt tccaatagac gaagtgccga acgcattact tcactggccg agccataacg
     7381 accagactgg accatccggg taataaaact atctaattgt tcgccaacgc ttactgaggt
     7441 aattttgcgc ataagcgatc tccacgtatt aactattaat acaaagtata tgtcagaacg
     7501 ctttctacaa ccaaaccgta ttcagttgcc tgtgaaacag gcaaaaaaaa accgcagcgc
     7561 aaatacgcca cggttttttt aacagaacgg atttaaaaca ccattacggc agaattgacg
     7621 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
     7681 gtttcagagc caacgcagat gccacataga tggaagatgc agtaccgatg gaaacaccga
     7741 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
     7801 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
     7861 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
     7921 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
     7981 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
     8041 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
     8101 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
     8161 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
     8221 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
     8281 gcatacggac catgatgtcg tggctgctac caaagttttg cagcatcggc tcttcaaaac
     8341 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
     8401 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
     8461 cgatagcagc gattaacagc agaccagaga tgccaaaagc ccagtagtcc cagcgcataa
     8521 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
     8581 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
     8641 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
     8701 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
     8761 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
     8821 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
     8881 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
     8941 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
     9001 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
     9061 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
     9121 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
     9181 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
     9241 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
     9301 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
     9361 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
     9421 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt aagaattacg
     9481 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
     9541 acgcgaccgg atgccgcagc ggcctgatca acgttggtgt ttaccagacg gaattccagc
     9601 gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt cctgaatacc tggcagttca
     9661 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
     9721 acacggttac gcaggatatt gatgttctgc tgcaccgcat attcacgcgc ttcactcaga
     9781 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct gactgctaat caccaggtcc
     9841 ggatgacgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
     9901 atgctcaggc cgtagttatt ttctttacga acggtggtat acgggatgcc tttttcgcgc
     9961 aggtcgctgc gcaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    10021 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    10081 gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    10141 ttgtcaccca taacgcccat taatgcttca cgagcgcgca gctgggtgtc agtggagtcg
    10201 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    10261 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    10321 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    10381 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    10441 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    10501 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    10561 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    10621 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    10681 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    10741 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    10801 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    10861 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    10921 caaacgctgg tagtaacgaa ggttatgaat ggtattgagt cgcgcgccta atatttcgtt
    10981 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    11041 ctcaggatcg agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    11101 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    11161 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    11221 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    11281 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    11341 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    11401 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    11461 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    11521 gatcatgacg atatccgaac caagatcgta ctgaatctcc atcgactttt ccgggtcgag
    11581 gaaaatcggg tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    11641 aatatcgcca aggctgaaga cttggaagcc gccggagtcg gtgaggatcg gtcccttcca
    11701 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    11761 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    11821 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    11881 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    11941 cattttttct ccactacgtc agaaaaacag tccaacgttt aaaccagcgc cgcggaatta
    12001 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    12061 actaaaaaag cgatatttct cttctaccgc cgctttatag gcattcattg tgtgttgata
    12121 accggcaaag gccgaaacca gcataatcag cgtcgattcc ggaaggtgga agtttgtcac
    12181 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcaaa
    12241 gaacggttcg atgagatcgt ttttcgccgc ctgagccgcg ctttccagcg aacgtactga
    12301 ggtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    12361 cacatcctgc ggtacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    12421 gcgtaccggc tggaaggtgc ccgcaccaac gtgcagggtg acgaacgcca tctccacgcc
    12481 tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc agaccagcgg tcggggctgc
    12541 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    12601 cggacggtcg atatacggcg gcagcggcat atggccgatg ctgttgagaa tatccagcac
    12661 cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    12721 gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc
    12781 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt tcaaccagca cttcaatctt
    12841 gccgccgctg gctttacgcc caaacaggcg cgccgggatc acccgggtat tattaaaaac
    12901 cagaagatcg ccggggttga gcttatcgag taaatcggtg aaagtaccgt gcgtcagcgc
    12961 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tcggatagtg
    13021 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    13081 gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    13141 ctttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    13201 aaagctcgct ttccggcaat ttgctggctg atttcgtacg cggaaatcct gaagaaagtt
    13261 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta ttgactgaca
    13321 atctgccgga agtccgcgaa gcacgggagt ggtttcgtag tgaaactcgc cgcgttgcgc
    13381 ctattacgct ggatgtcatg tgggatcact ttctttcccg tcactggtcg cagctgtcgc
    13441 cggactttcc gctacaggaa tttgtttgtt atgcccgcga gcaagtgatg acaattttgc
    13501 cggactcacc gccacgcttt atcaacctga acaactactt atggtcagag cagtggctgg
    13561 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgtcgcc
    13621 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgcccattat gccgccctcg
    13681 aaacccgctt ctggcagttt tatccacgga tgatggcgca ggcgtcacgc aaggcgctct
    13741 gaaacttcaa actccggcgt cactattttt tagcattcgc atccagcggg gcggcataac
    13801 aacggtaaga aaaacattcg ctggaatgat tggcgatatc tttaaagttg ctttcagcaa
    13861 ccccctcgaa catgagtacc gttctggaca actctccgtt ttcatttata tgtggaaaat
    13921 caaattggct actgaatcgc catacatctg tcttttctgg ctcgctcttc acagagatat
    13981 gtcgatggag gccccctcct gtaaacgtgc gccagaacaa ttgttgatca aaatcttgta
    14041 atatcgcaga cttggatgct atttgacgta gtaaacctgc ataccgtgga ctgccattac
    14101 gagcaacatt atgcaaaccc tgactaaaag catcaaacaa acaatagatg tgatcactag
    14161 tgaaaaaagg tcttaatttt ttttgttctt catcgctgta atccaggagc atctcattga
    14221 tttcttccgg tgagaatttt aagacaaaat tatcctcaga atttgtttgt ttcttgtttt
    14281 tttcaatatt cttctcctca atgctcttcg cattttgttt cgcaattatt tccgcagttt
    14341 ttttcgcaat ttcttccgca attttttccc gagttattga ttttagacta acaccatcga
    14401 tgtcaaccgg tgttgttttc agatctctct gcgcgtcttc caaatttgtc ttatagagtt
    14461 tgtgcagttt atcgg
//
LOCUS       DBFIFS010000115        14396 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.115, whole genome shotgun sequence.
ACCESSION   DBFIFS010000115 DBFIFS010000000
VERSION     DBFIFS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.115"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            105..1676
                     /gene="garD"
                     /locus_tag="N7A07_002979"
     CDS             105..1676
                     /gene="garD"
                     /locus_tag="N7A07_002979"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU2477605.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1825..2160
                     /gene="prlF"
                     /locus_tag="N7A07_002980"
     CDS             1825..2160
                     /gene="prlF"
                     /locus_tag="N7A07_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU2477606.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2160..2624
                     /gene="yhaV"
                     /locus_tag="N7A07_002981"
     CDS             2160..2624
                     /gene="yhaV"
                     /locus_tag="N7A07_002981"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU2477607.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2679..3488)
                     /gene="agaR"
                     /locus_tag="N7A07_002982"
     CDS             complement(2679..3488)
                     /gene="agaR"
                     /locus_tag="N7A07_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU2477608.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3737..5017
                     /gene="kbaZ"
                     /locus_tag="N7A07_002983"
     CDS             3737..5017
                     /gene="kbaZ"
                     /locus_tag="N7A07_002983"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU2477609.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5040..5513
                     /gene="agaV"
                     /locus_tag="N7A07_002984"
     CDS             5040..5513
                     /gene="agaV"
                     /locus_tag="N7A07_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU2477610.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5524..6303
                     /gene="agaW"
                     /locus_tag="N7A07_002985"
     CDS             5524..6303
                     /gene="agaW"
                     /locus_tag="N7A07_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU2477611.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6293..7171
                     /gene="agaE"
                     /locus_tag="N7A07_002986"
     CDS             6293..7171
                     /gene="agaE"
                     /locus_tag="N7A07_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU2477612.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7189..7623
                     /gene="agaF"
                     /locus_tag="N7A07_002987"
     CDS             7189..7623
                     /gene="agaF"
                     /locus_tag="N7A07_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU2477613.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITSTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7620..8753
                     /gene="nagA"
                     /locus_tag="N7A07_002988"
     CDS             7620..8753
                     /gene="nagA"
                     /locus_tag="N7A07_002988"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU2477614.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9104..10258
                     /locus_tag="N7A07_002989"
     CDS             9104..10258
                     /locus_tag="N7A07_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU2477615.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10271..11131
                     /gene="kbaY"
                     /locus_tag="N7A07_002990"
     CDS             10271..11131
                     /gene="kbaY"
                     /locus_tag="N7A07_002990"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU2477616.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11298..11774
                     /gene="agaB"
                     /locus_tag="N7A07_002991"
     CDS             11298..11774
                     /gene="agaB"
                     /locus_tag="N7A07_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU2477617.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11813..12616
                     /gene="agaC"
                     /locus_tag="N7A07_002992"
                     /pseudo
     CDS             11813..12616
                     /gene="agaC"
                     /locus_tag="N7A07_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
     gene            12606..13397
                     /gene="agaD"
                     /locus_tag="N7A07_002993"
     CDS             12606..13397
                     /gene="agaD"
                     /locus_tag="N7A07_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU2477618.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13398..14153
                     /locus_tag="N7A07_002994"
                     /pseudo
     CDS             13398..14153
                     /locus_tag="N7A07_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         3491 a         3626 c         3744 g         3535 t
ORIGIN      
        1 tgcgtttaaa ctcctgacat tcttatttca cccaatgaag tcatttattt ttaaatgaga
       61 ccaggtcctc attttaataa cctctggctg gagaatattg cacaatggcc aacatcgaaa
      121 tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa
      181 ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatggg ctggaattaa
      241 ttgaacatat tccccagggg cataaagtcg cattgctcga cattccggct aatggtgaaa
      301 ttattcgtta tggcgaagtg attggttacg ccgtgcgtgc aatcccacgc ggaagctgga
      361 tcgacgaatc aatggttgta ctgccgaaag cgccgccgtt acacacgctg ccactggcaa
      421 ccaaagtccc ggaaccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg
      481 ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg
      541 caggcgtggt ggactacgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga
      601 acgtcgatgg cgtggtgggg ctgaatcatt tgtacggttg tggcgtggcg attaacgcac
      661 cggcggcagt tgtacctatc cgtaccattc acaatatttc gctgaatcct aactttggcg
      721 gcgaagtaat ggtgattggc ctgggttgtg aaaagttgca gcctgagcgc ctgctgactg
      781 gaacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg
      841 aaaagcatgt cggttttcag tccatggtcg aggatatttt gcaggtcgcc gaacgccatc
      901 tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg gttgtcggca
      961 tgcagtgcgg tggcagcgat gccttttctg gcgtaacggc aaacccggcg gttgggtatg
     1021 cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ctcagaagtc acggaagtgc
     1081 gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc aaacggctgc
     1141 tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac cgcagcgcca
     1201 acccttcgcc gggcaacaag aaaggcggtc tggcaaatgt agtggagaaa gcgctcggct
     1261 ccattgctaa atcgggtaaa agtgcaattg ttgaagtgct gtcgcccggt caacgcccga
     1321 ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac
     1381 aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc
     1441 tgatggcggt acccgtcatt aaaatggcga cccgcaccga gctggcgaac cgctggtttg
     1501 atttaatgga tattaacgcg ggcactatcg ccaccggcga agaaaccatt gaagaggtgg
     1561 gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa accttctcgg
     1621 atcaatgggg actgcataac cagctggcgg tgtttaaccc agcaccggtg acctgatttc
     1681 ttttggaacg tccctcgcaa aacatggcct tagtgccatg tttttattgt ttaaagcccc
     1741 cacgtccatt aataatgcat ttgcattacc ttagttcaag cttataattt gagcagaaaa
     1801 caggctgtaa aaggacagtg aatcatgccc gctaatgctc gctctcacgc tgtactgacc
     1861 actgaatcaa aggtcacgat acgcggacaa acaactatcc ccgcgccagt gcgtgaggcc
     1921 ttaaaactga agccaggcca ggacagcatt cattacgaaa ttctgcctgg tgggcaagta
     1981 tttatgtgcc gactgggaga tgagcaggag gatcatacta tgaatgcatt tttgcgtttt
     2041 ctggatacag atatccagaa caacccgcaa aaaactcgtc cattcaacat tcaacaagga
     2101 aagaaacttg tcgctggcat ggacgtcaac attgatgatg agattggcga cgacgaataa
     2161 tggattttcc acaaagggtt aatggttggg cgctatatgc tcatccctgt tttcaggaaa
     2221 cctacgacgc tttagttgcc gaagtcgaga cattaaaggg aaaagatcct gaaaattatc
     2281 agagaaaagc tgccacaaag ttattggcgg tggtccataa agtgattgag gagcatatca
     2341 cggtcaatcc atcatcaccg gcattccgtc atggcaagtc gttaggctct gggaaaaata
     2401 aagactggtc acgggtaaaa tttggtgctg gtcgctatcg tctcttcttt cgttacagtg
     2461 aaaaagagaa agtcatcatt ctgggatgga tgaacgatga aaacacgctg cgcacctacg
     2521 gtaaaaaaac agatgcctat accgtattca gcaaaatgtt aaaaagagga catcctcctg
     2581 ccgactggga aaccctcacc cgagagacag aagaaaccca ttgatggtgt tcacaatgcc
     2641 cgccgaacgc attcgtgttg accagcgggc accctttctc actccccgac cagaatcact
     2701 tcaaccccag cctttcgcag cccttccagg ctatccgcag gaatgccttc atcaacaatg
     2761 atcatgtcga tacgttgagt atcaatgatc ttatgtaaac tggaacgatt gaatttactg
     2821 gaatcggtga ccacgatgat ccgttccgca acttcgcaca tccggcggtt taaacgggct
     2881 tcatcttcat tatgcgtgct gacgccgcgc tccagatcga tcgcatctac accaagaaac
     2941 agcatatcga agtggtaatt ttgcagcgat tgctcagcct gatcgccgta aaaagattgc
     3001 gactgacggc gcaaatgccc gccggtcatc agcagctcaa cgccttccgc ttccagcaac
     3061 gcattagcca cgttcatacc gttggtcatc gcaattacgt cagtgtgctt gcgcatcaga
     3121 cgagcaatct caaaagtggt ggtcccggaa tcgaggatca cccgatgacc tggctgaatc
     3181 aactcaacgg cagctttcgc aacgctgcgt ttcattgcgg tgttcagcgc gcttttatct
     3241 tccactgatg gctcgactga cggcgttgtg ctatcgcaga tcaacgcgcc accataggca
     3301 cgcacagcga tcccctgctt ttccagaaac gccagatcgt tgcggatcgt cacggtagat
     3361 acgccataca atgccgacag atcgttaacc tgcacactcc cttgctgtcg cagacgctga
     3421 atgatctgtt ctcgtcgctc gctggtgcct gtcactcgct tctcacctga agcgtcggta
     3481 ttactcatag taagtccttt cgtaaaactt tcgtttcatt tcgttttgcc tattaacgcc
     3541 tttctattaa gcaaatgcaa gcccaccttg cccattgacg caagctactc tcgtttcagt
     3601 gactttcatt atgtttcttt tgtgaatcag atcagaaaac tattatcttt cgttttattt
     3661 ttatctcacc atgacgcagt atcaactgaa acaaaacgaa agattaatat cgcagtaatc
     3721 tgaactggag aggaaagtga aacatctgac agaaatggtg agacagcaca aagcgggcaa
     3781 aacaaatgga atttatgccg tttgttccgc acatccgctg gtgctggagg ctgcaatccg
     3841 ctacgccagt gcaaaccaaa cgccgttact gattgaagca acctccaatc aggtagacca
     3901 gttcggcggt tataccggaa tgacgcccgc cgattttcgc ggctttgttt gtcagctcgc
     3961 cgactcgttg aatttcccgc aggatgcgtt gattctgggt ggtgaccatc tggggccaaa
     4021 ccgctggcaa aacctgccgg ccgctcaggc aatggccaat gccgatgatt tgattaaaag
     4081 ctacgttgcg gcaggattca aaaaaatcca ccttgattgc agcatgtcct gtcaggacga
     4141 tccgattccc ttaactgatg acatcgtggc tgaacgcgcc gcccgtctgg cgaaagtggc
     4201 ggaagaaacc tgtcttgaac actttggcga agccgatctg gagtatgtca ttggtaccga
     4261 agtgccggta cctggcggcg cgcatgaaac cttaagcgag ctggcggtca ccacgccgga
     4321 tgccgcccgc gccacgctgg aagcccatcg tcacgccttt gaaaagcaag gtttgaatgc
     4381 catctggcca cgcatcattg ccctggtggt tcaacccggc gtcgaattcg atcacaccaa
     4441 cgttattgat tatcagcccg ccaaagcgac cgccttaagc cagatggtcg aaagctacga
     4501 aacgctgatt ttcgaagcgc actctaccga ttatcaaacg ccgcaatcgc tgcgccagct
     4561 ggtgattgac cactttgcca ttctgaaagt tggcccggcg ctgaccttcg ccctgcgtga
     4621 agctctgttc tctctggcag cgattgaaga agaactggtg ccagcaaaag cctgttctgg
     4681 tctgcgtcag gtgctggaaa acgtgatgct caaccgcccg gaatactggc aaagccacta
     4741 ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttat agctactcgg atcgcgtgcg
     4801 ctattactgg ccggacagcc agattgatga cgctttcgct catctggtac gtaatctggc
     4861 ggattcacca attccgctgc cgctgatcag ccagtatctg ccgctgcagt acgtgaaagt
     4921 tcgctccggc gagctgcagc caacgccacg ggaactcatt atcaaccata ttcaggacat
     4981 cctggcgcag taccacacag cctgtgaagg ccaataagca aaacaaagag gaacacgcta
     5041 tgccaaatat tgttttaagc cggattgatg aacgcttgat tcacggtcag gtcggcgttc
     5101 agtgggtcgg atttgcgggg gcaaatctgg tgctggtcgc caacgatgag gttgccgaag
     5161 atccggtaca acaaaacctg atggaaatgg tactggcaga agggatcgcc gtacgtttct
     5221 ggacgctgca aaaagttatc gacaacattc atcgcgccgc cgatcgacag aaaatcctgc
     5281 tggtttgtaa aacacccgcc gatttcctga cgctggtgaa aggtggcgtt ccggtgaatc
     5341 gcattaacgt tggcaatatg cactacgcca atggcaagca acaaatcgcc aaaacggttt
     5401 ctgtggatgc gggcgatatc gcagcattta acgacctgaa agccgctggg gtggaatgct
     5461 tcgttcaggg catcccgaca gagcctgctg tggacctctt taaattactt tgagggattc
     5521 atcatggaaa tcagtctgtt gcaggcattc gcgttgggca ttatcgcctt tatcgctggc
     5581 ctggatatgt ttaacggctt aacccatatg caccgcccag tggtcctcgg cccgttagtc
     5641 gggctggtac ttggcgatct gcataccggt attttaaccg gcggtacgct ggaactggtg
     5701 tggatggggc tggccccgct ggcgggcgca cagccgccta atgtgattat cggtactatc
     5761 gtcggcacgg cgtttgccat tactactggc gtgaaacccg atgtcgcagt cggtgtcgcc
     5821 gtacctttcg ctgtcgcagt acagatgggg attaccttcc tgttctcggt gatgtctggc
     5881 gtgatgtctc gctgcgaccg gatggcggag aacgccgata ctcgcggcat tgaacgcgta
     5941 aactatctgg cactgctggc actcggcact ttctattttc tctgcgcttt cctgcctatc
     6001 tacttcggcg cggaacatgc aaaaaccatc attgatgtcc tgccgcaacg attaatcgac
     6061 ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct gctgaaaatc
     6121 atgatgaaaa acgtctacat cccctacttc atcctgggct ttgttgccgc agcctggctc
     6181 aagttaccgg tgctggctat tgctgccgcc gcgctggcga tggcgctgat cgatctgctg
     6241 cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag aggaatttga agatggcatc
     6301 taatcaaacc accctgccga acgtcagtga aaacgaagaa acattgctga ctggcgtcaa
     6361 tgaaaacgtg tatgaagatc agagcattgg cgcggagctg acgaaaaaag atatcaatcg
     6421 cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac tacgaacgta tgcaggcttc
     6481 cggctggttg tacggtctgc tgcctgctct gaaaaagatc cacactaata aacgcgacct
     6541 ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc catccgtttc tggtgacatt
     6601 tgttatcggc attatccttg cgatggagcg ttctaagcag gacgttaaca gtattcagag
     6661 caccaaaatt gccgtcggtg cgccgctcgg cgggattggc gatgcaatgt tctggctaac
     6721 gctactgccg atttgtggcg ggattggagc cagtctggca ttgcaaggct ccattcttgg
     6781 cgcagtcgtc tttattgtgc tgttcaacgt ggtccacctg gggctgcgtt ttggtctggc
     6841 gcattatgct taccgcatgg gcgtggcggc gattccactc attaaagcta ataccaaaaa
     6901 agtcggccat gctgcgtcta tcgttgggat gacggtaatc ggtgcgctgg tggcaaccta
     6961 tgttcgttta agcaccacgc tggaaatcac agcgggcgac gcagtggtta agttacaggc
     7021 tgatgttatc gacaaactga tgcctgcctt cttaccgttg gtctacaccc tgaccatgtt
     7081 ttggttggta cgccgcggct ggagtccgct gcgtctgatt gcagttaccg tggttctcgg
     7141 catcgttggt aaattctgcc atttccttta aatacaatga ggtattagat gttaagtatt
     7201 attttgacag ggcatggcgg gtttgccagc ggcatggaaa aagcaatgaa gcaaattctc
     7261 ggtgagcaat cgcagtttat cgccatcgat tttccggaaa cctcaagcac cgcgctgctt
     7321 acatcgcagc ttgaagaggc catcgcccaa ctggattgtg aagacggcat tgtttttctg
     7381 acggatttgc tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat gcaaaaaccg
     7441 ggctgtgaag tgatcaccag caccaatttg caactgctac tggagatggt gctggagcgc
     7501 gaagggttaa gcggtgaaga attccgtgta caggcgctgg agtgtggaca tcgtgggctg
     7561 accagtctgg tggacgagtt aggccgctgc catgaagaat gtccggtcga ggaagggata
     7621 tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga gggatggctc gatgaccatc
     7681 agttgcgtat tgctgacggt gtcatcgcag caatcgaacc gattccagtg agcgtgactg
     7741 aacgcgatgc ggaactgctc tgccccgctt acatcgacac ccatgtacac ggtggtgcgg
     7801 gcgttgatgt aatggatgac gcgccggatg tactcgacaa gctggcaatg cacaaggcac
     7861 gcgaaggtgt cggcagttgg ttgccgacta ccgtaaccgc gccgcttagt accattcatg
     7921 cggcgctgaa acgtattgct caacgttgcc aacgcggcgg acctggtgcg caagtgctgg
     7981 ggagttatct cgaaggaccg tacttcacgc cgcagaataa aggcgcgcat ccgccggagt
     8041 tgtttcgcga gcttgaaatt gccgagctgg atcaattgat tgccgtttct cagcacacct
     8101 tacgcgtggt agcgctggca ccggaaaaag agggggcatt gcaggccatc cgccatctta
     8161 aacagcaaaa tgtacgagtg atgctggggc atagcgcggc gacctggcaa caaactcgcg
     8221 ccgcgtttga tgctggtgcc gacggcctgg tgcattgcta taacgggatg acaggtttac
     8281 atcaccgcga accgggaatg gttggcgcgg gattaacgga caagcgcgcc tggctggaac
     8341 tgatagccga tggtcatcat gtgcatccgg cggcaatgtc gctgtgttgt tgctgtgcga
     8401 aagagagaat cgtactgatc accgacgcga tgcaggcagc cgggatgccg gatggtcgct
     8461 atacgttatg tggcgaagaa gtgcagatgc acggtggcgt tgtccgtacc gcgtccggtg
     8521 ggctggcggg cagtacgctg tctgttgatg cggcagtgcg caacatggtc gagttgacgg
     8581 gcgtaacgcc tgcggaagcc attcatatgg cgtcgctgca tccggcgcga atgctgggtg
     8641 ttgatggtgt tctgggatcg cttaaaccgg gcaaacgcgc cagcgtcgtt gcgctggata
     8701 gcgggctgca tgtgcaacaa atctggattc agggtcaatt agcttcgttt tgatggtttg
     8761 ctccattatt gggccttcac ttcccccgta aggcctttct ttttctttcg ttttgatctg
     8821 tgcagcggtg tcggatgcga cgctaacgcg tcttatccga cctacagttg gtgaccgcaa
     8881 ggccggataa agcgtttgcg ccgcatccag caatcccttt tgcttccttt atcttttctt
     8941 tcaacgatca cggatttcgt tttatttcct ttttctccat tgaactttcg gtttcttttc
     9001 tatagatttt aatcaacgaa agacatcacc aagtgaaatg aaacgaaagg caagtgaaag
     9061 cgacaacgcc cgacgtcaag ttcatcagac taaggattga gttatgccag aaaattacac
     9121 ccctgctgcc gccgcaaccg gtacatggac tgaagaagag atccgccatc agcctcgcgc
     9181 atggatccgt tcgctcacca acatcgacgc gctacattcc gcgcttaata acttccttga
     9241 accgttactg cgcaaagaga atctgcggat catcctgacc ggagccggaa cctcggcatt
     9301 tatcggtgac atcatcgcgc cgtggctcgc cagccatacc ggtaaaaact tcagcgccgt
     9361 accgaccacc gatctggtca ctaatccgat ggactacctg aacccagccc atccgctgct
     9421 gttgatctcc ttcggtcgat ccggcaacag cccggaaagc gtcgcagccg tggaactggc
     9481 aaatcaattt gtaccggaat gctatcacct gccgatcacc tgcaacgaag cgggcgctct
     9541 ttaccaaaac gcgatcaaca gcgataacgc gtttgccgtg ctgatgcccg cagaaacgca
     9601 cgatcgcggc tttgcgatga ccagcagcat taccaccatg atggccagct gcctcgcggt
     9661 tttcgcacct gagacgatca acagccaaac cttccgcgac gtggcggatc gttgccaggc
     9721 gatcctgacc tcactgggcg atttcagcga aggtgtgttt ggttacgcac cgtggaaacg
     9781 gatcgtttat ctcggcagcg gtggcttaca gggcgcagca cgcgagtcgg cgctgaaagt
     9841 gctggaactg acggcgggta aactggcggc cttttatgat tctccaaccg gattccgtca
     9901 tggaccaaaa tcgctggtcg ataacgaaac actggtggtg gtatttgtct ccagccaccc
     9961 ttacacccgt cagtatgatc ttgatctgct ggctgaactt caccgtgaca accaggcaat
    10021 gcgtgtaatc gccatcgccg cggaaagcag cgacatcgtc gctgccggtc cacatatcat
    10081 cctgccaccg tcacgtcact ttatcgacgt tgagcaggca ttttgcttcc tgatgtacgc
    10141 ccagacgttt gcactgatgc agtcgctgca catgggcaat acgccggata ccccatcagc
    10201 cagtggcacc gttaaccgcg tggtgcaagg cgtaatcatt catccgtggc aggcataaga
    10261 ggatcgcatt atgagcatta tctccaccaa atatctgtta caggacgccc aggccaatgg
    10321 ctacgcggtg cctgctttta acattcataa cgccgagacg atccaggcga tcctcgaagt
    10381 gtgcagtgaa atgcgatcgc cggtgatcct cgccggaacg ccggggacct ttaaacatat
    10441 cgcgctggaa gagatctacg ccctgtgcag cgcctattcc acaacctaca acatgccact
    10501 ggcgctgcat ctcgaccacc acgaatcgct ggatgatatt cgccgtaaag tccacgcagg
    10561 tgtgcgcagt gcgatgatcg acggcagcca cttcccgttt gccgagaacg taaagctggt
    10621 gaaatcggtt gtcgacttct gccactccca ggattgcagc gtggaagcag aactgggccg
    10681 cctgggcggt gttgaagatg acatgagcgt tgacgccgaa agtgcattcc tgaccgatcc
    10741 acaagaagct aaacgctttg tcgaactgac tggcgtcgac agcttggcgg tagcgattgg
    10801 tacggcgcac ggcttataca gcaaaacgcc gaagattgat ttccagcggc tggcggaaat
    10861 tcgtgaagtg gtggatgttc ctctggtgct acatggtgcc agcgatgttc cggatgaatt
    10921 tgtccgtcgc actattgaac ttggcgtcac aaaagtgaac gttgccacag aattaaaaat
    10981 agccttcgcc ggcgcggtta aagcctggtt tgcggaaaat ccgcagggta atgatcctcg
    11041 ttattatatg cgcgtcggaa tggatgcgat gaaagaagtt gtcagaaata aaattaatgt
    11101 ctgtggttca gcgaatcgaa tttcagcata atcattagtt ttttatgatt tatcccaatg
    11161 tacttcccga ttaatatcgg ggagtgcctt aatggaaaag gagatgacta aaccttaata
    11221 aatagatcag tacaatatcg caacaataat atatttaaaa aaattatatt attcaacttt
    11281 atggtgagga ttacacaatg agcagtccaa atattctctt aacccgtatt gataaccgtc
    11341 tggttcacgg tcaggttggc gtgacctgga catccaccat cggtgcgaat ctgctggtag
    11401 tcgtggatga tgttgtcgct aacgatgata ttcaacagaa attaatgggt attaccgcag
    11461 aaacctacgg ctttggcatt cgtttcttta ctatcgaaaa aaccattaac gtcatcggca
    11521 aagctgcacc gcatcagaag atcttcctga tttgccgtac gccacaaacg gtacgtaaat
    11581 tggtagaagg tggcattgac ctgaaagatg tcaacgtcgg caatatgcat ttctcggaag
    11641 ggaaaaagca aatcagcagt aaagtttatg tcgatgacca ggatctcacg gacttacgtt
    11701 ttattaaaca acgtggcgtg aatgttttca ttcaggacgt ccctggcgat caaaaagaac
    11761 aaatccctga ctaaatctaa aatcgcctta atattggttt gaggtaataa aaatgcatga
    11821 aataacccta ctttagggat tatccctagc ggcgttagtt ttcgttctgg ggattgattt
    11881 ttggctggaa gccttatttt tattccgccc gataatcgtt tgtaccctaa ctggcgctat
    11941 tctcggtgat attcagactg gcttaattac cggtggtctg acagagttgg ctttcgccgg
    12001 attaacccct gcaggtggtg ttcagccgcc caacccgatt atggcgggtc tgatgaccac
    12061 cgtcattgca tggtctacgg gcgttgatgc caaaacggca attggtcttg gcctgccgtt
    12121 tagtttgtta atgcagtacg tcattctgtt cttctattct gctttctcat tatttatgac
    12181 caaagccgat aaatgcgcga aagaggcgga tacggcagcg ttttcccgac ttaactggac
    12241 aacgatgctc atcgtcgctt cagcgtatgc ggtgattgct ttcctctgta cttacctggc
    12301 acaaggggcg atgcaggcgc tggtgaaagc gatgcccgct tggctgaccc acggctttga
    12361 agtggctggc ggtattctgc ctgccgttgg ttttggcttg ctgctgcgcg tgatgttcaa
    12421 agcacaatat atcccttacc tgatcgccgg cttcctgttt gtttgctaca tccaggtcag
    12481 caacctgttg ccggttgccg tactgggcgc aggctttgcg gtgtatgagt ttttcaatgc
    12541 gaaatcccgg cagcaagcgc aaccgcagcc cgttgccagt aaaaatgaag aagaggacta
    12601 cagcaatggg atctgaaatc agtaaaaaag atatcacccg tctgggcttt cgttcgtcgc
    12661 tgctgcaagc gagctttaac tacgaaagga tgcaagcggg cggttttacc tgggcgatgt
    12721 tgccgatcct gaaaaagatt tataaggatg acaaaccggg cctaagcgca gcgatgaaag
    12781 ataacctcga atttattaat acccacccga atctggtcgg attcctgatg gggttattaa
    12841 tttcgatgga agaaaaagga gaaaaccgcg acaccattaa aggcctcaaa gtggcactgt
    12901 ttggcccaat cgccgggatt ggcgatgcga ttttctggtt tactttgttg ccgattatgg
    12961 cgggaatttg ctcatcattt gccagccagg gaaacctgct ggggccgatt ctatttttcg
    13021 ccgtttacct gcttatcttt ttcctgcgcg tcggctggac ccacgtcggt tattcagtcg
    13081 gcgtgaaggc gatcgataaa gtgcgagaga actcgcagat gattgctcgc tcggcaacca
    13141 tcctcgggat cacggtaatc ggcgggctga tcgcttcgta tgtgcatatt aacgtggtga
    13201 catcgtttgc catcgacagt acccacagcg tcgcgctgca acaggatttc ttcgataaag
    13261 tcttcccgaa cattttaccg atggcctaca ccctgctgat gtattacttc ctgcgggtga
    13321 aaaaagcgca tccggtgctg ttaatcggcg tgacttttgt gctctctatt gtttgttccg
    13381 cattcggcat tttgtaaatg gaacgaggca ctgcgtctgg cggtgcctct ttacttaagg
    13441 aatttcatcc tgtgcaaacc cttcagcaag ttgaaaacta tacggcgtta agtgaacgtg
    13501 ccagcgaata tttattggcc gtgatccgta gcaaaccgga tgccgtgatt tgcctggcga
    13561 ccggagccac gccattactg acgtatcatt atctggtaga aaaaatccac tagcagcagg
    13621 ttgatgtcag ccagctcacc ttcgtgaagc tcgacgaatg ggtggatctg ccattaacga
    13681 tgcctggtac ctgcgaaact ttcctgcaac agcatatcgt gcagccgctg gggctacgtg
    13741 aagaccagct catcagcttt cgctccgaag agataaatga gacagagtgc gaacgggtaa
    13801 cgaacctgat tgcgcgcaaa ggcggtctgg atttatgcgt tctcggattg gggaaaaacg
    13861 gtcatcttgg gctgaacgaa ccgggagaaa acctgcaacc ggcctgccat atcagtcaac
    13921 ttgatgccag aacacagcaa catgagatgt taaaaaccgc gggtcgcccc gtgactcgtg
    13981 ggatcacctt aggcctgaag gatattctca atgcccgcga ggttttgtta ctggtgactg
    14041 gcgaaggaaa gcaggatgcg acagaacgtt ttctcacggc taaagtctct acctctatcc
    14101 cggcttcgtt tttgtggctg cacagtaact ttatttgttt aattaatacc taaccagatg
    14161 aaaaatttgc tcaaaatgtt taaattaact tatgcaacag tcacgcatta tattaaataa
    14221 catttgactg ggttgaacat aacgccaata gcaaaaggag ttatgttcaa caatttaatt
    14281 ttagatatac atataacttg ttctattaat aaaccatctc gtattctgac tcgccccctg
    14341 gcgcagcaaa gtcatgcact tatactcttt tgaatacata gaaaattgat atcaat
//
LOCUS       DBFIFS010000116        14138 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.116, whole genome shotgun sequence.
ACCESSION   DBFIFS010000116 DBFIFS010000000
VERSION     DBFIFS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.116"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..132
                     /locus_tag="N7A07_002995"
     CDS             <1..132
                     /locus_tag="N7A07_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HGU2477619.1"
                     /translation="PKKYDSDVSSWLGRNRDKILEIWNALQAGAPYEPLIAELTGGV"
     gene            complement(259..2178)
                     /locus_tag="N7A07_002996"
     CDS             complement(259..2178)
                     /locus_tag="N7A07_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HGU2477620.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVELI"
     gene            complement(2269..2353)
                     /locus_tag="N7A07_002997"
     tRNA            complement(2269..2353)
                     /locus_tag="N7A07_002997"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2317..2319),aa:Leu,seq:caa)
     gene            2549..3568
                     /gene="ahr"
                     /locus_tag="N7A07_002998"
     CDS             2549..3568
                     /gene="ahr"
                     /locus_tag="N7A07_002998"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU2477621.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3698..5200
                     /gene="yjgR"
                     /locus_tag="N7A07_002999"
     CDS             3698..5200
                     /gene="yjgR"
                     /locus_tag="N7A07_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU2477622.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLVRLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5361..6443)
                     /gene="lptG"
                     /locus_tag="N7A07_003000"
     CDS             complement(5361..6443)
                     /gene="lptG"
                     /locus_tag="N7A07_003000"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU2477623.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6443..7543)
                     /gene="lptF"
                     /locus_tag="N7A07_003001"
     CDS             complement(6443..7543)
                     /gene="lptF"
                     /locus_tag="N7A07_003001"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU2477624.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7606..7656)
                     /gene="ytgA"
                     /locus_tag="N7A07_003002"
     CDS             complement(7606..7656)
                     /gene="ytgA"
                     /locus_tag="N7A07_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU2477625.1"
                     /translation="MSIIILATISTTQENK"
     gene            7810..9321
                     /gene="pepA"
                     /locus_tag="N7A07_003003"
     CDS             7810..9321
                     /gene="pepA"
                     /locus_tag="N7A07_003003"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU2477626.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9675..10118
                     /gene="holC"
                     /locus_tag="N7A07_003004"
     CDS             9675..10118
                     /gene="holC"
                     /locus_tag="N7A07_003004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU2477627.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10118..12973
                     /gene="valS"
                     /locus_tag="N7A07_003005"
     CDS             10118..12973
                     /gene="valS"
                     /locus_tag="N7A07_003005"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU2477628.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13028..>14138)
                     /gene="yjgN"
                     /locus_tag="N7A07_003006"
     CDS             complement(13028..>14138)
                     /gene="yjgN"
                     /locus_tag="N7A07_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGU2477629.1"
                     /translation="VNVLLTIITLGIYLPWALMKCKRYLYANMEVNGQRFSYGITGGN
                     VFFSCLVFVFFYFAILMTVSADMPLIGCVLTLSLLVLLIFMAAKGLRYQALMTSLNGV
                     RFSFNCSMKGVWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIVSVVLMAIVGIV
                     SIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGYI
                     IFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHF
                     MSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYL
                     LGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3650 a         3599 c         3659 g         3230 t
ORIGIN      
        1 cctaagaaat atgatagtga tgtttcgagt tggttgggga gaaatcgtga taaaatttta
       61 gaaatctgga atgcactaca agctggcgca ccttatgaac cactaatcgc agaattgact
      121 ggcggagtgt gactaactaa ataattttcc cctaaaaaaa ttcgatgtag gaaaatatat
      181 ataagtaaga atcatgagtg tccgctcctc gctcaaggcg gactgttaga cttgattgta
      241 tgctgtcaga gaaaaccgtc agatcaattc tacagcatcc agcccatcag gaatgttgaa
      301 tctcgctatc tcgggcaaag aaaaatcaat cttttcgaca acctcaacca tgtgaaccgg
      361 ttttaaatct ttgctgtatc gcacttcagt ttctgttttg cctcgcacat gcccaagtag
      421 ctgcccacgc agttccgaac taacaccctt ctctttaagc aaagttgaaa cagaatgccg
      481 gaaagagtgg aaagatttgc ttctgtctct ttcaagtttt aatcgacccg caagcaatga
      541 ttcattgaac catgcggaaa ccggcctacc atagcctttg gttttatgtg gtttaagctc
      601 gggaaaaaga cggtcatacc cggcagcttt caacgcatcc acatactcga taaaaccgag
      661 ttcaatcagc gtagaatgaa ccggaatctt gcggcgggaa ttggcgtttt ttagtttctt
      721 tccgttttcg ctatcgcttt caaggttcag gtaataaacg ccgtcatctg ccagaacatc
      781 gtccagcatt aactgtgcaa tctcgttaac ccttgccccc gtcatcaagc ccaacaacgg
      841 cgcccaatag tgaaacgggc aatattgatg gaatcgccct tgcgcattac gttcaacagt
      901 tccagcgaca aaccacggtt gcgaaaaaat tgccttaatt tcatgcggtt caaatatgtc
      961 agtgtgatcc tgctctcgct cacctttgtc atcgggaggg aaaaagttgt cgcatggcga
     1021 tttcagaaac ttaccatcat caacagccca ctccattgca ccataaaggc cgttgatata
     1081 ctttttgaca gactcttctg tcatcagttt atcgccacat tctgccgctt tggcgattaa
     1141 ctcatccagc gtcttaaccc cgtgcttacc tttcatcaaa ttacggttag caggaattgt
     1201 gcgcaacttg ctctcataag cccggaggaa atcacggtcg aaatcttcca actgaatatc
     1261 ccccatcact tcaatcatca aatcgaagta atagcgaata tcgcggagcg ccgcttctga
     1321 taccttgccc ttacgtcgat gtttcaaata ctcttcacga agccatgaca agttttcatt
     1381 gtaatgtttg cggtgaacat attcattagc gataacagga gcgacaagag gcacggcagg
     1441 gtgaatgcta actgctggtg gcgtggtctt aaccagccat gtaagtcgaa ggccttcagc
     1501 atgaactttg ctaatcaaca ggctatcagc agttatatct acgccgggta aatcgaagaa
     1561 ccaacctcca ctttggttat caccactagt tcgaaatgca gcctctcctt cggatactcc
     1621 agcgctcaac agacaatcta aagtatgccg gggattgaag gggcggagat aaccatttgc
     1681 gaagctctcg atacccagct caaacgcgct attgagaatg atgacttctt taggttgatc
     1741 cttttcatca tcagactgtt tttctcgttt tgcacccggt tcccgctcta ctgcattaaa
     1801 ttcatcaaca tagaaaccga actgaccagt cgccagccag aataccggga ggttacgatt
     1861 aaccacctct cgaatcaatt gcgatatagg caactcgatt gactgagcaa agttagaaag
     1921 cgagatcata ccctcaccag caagcagtgg ggcgcagtca cttaaagttc gataatccaa
     1981 ttgttcatac tcttgtaagg tctgatacca gaccgccaac aggagcgcgg acttctgctt
     2041 tgcttcatga agatcgtgag tgcctgttga agtgtgaatc tcacactgtc ctgcgtatcg
     2101 acgaaaacgt tggggaacgc taatgcggac aacataaatg ccgctggttc gacgatagag
     2161 tttatatcgc tgagacatcg cctcgtatac ccttttgtaa gaggggctac gcgatttcaa
     2221 gcaagattat gaattaaatt gttttaaaac aatagtttag atgagtcttg gtgccgaagg
     2281 ccggactcga accggcacgt atttctacgg ttgattttga atcaactgcg tctaccgatt
     2341 tcgccacttc ggcactgaag aggtatgcgg aaaacgttgt ggattatacc tgtcgcacgc
     2401 caccatgcaa gcgccagctt gctcgcacct cgctaagtgc tgaaattttc agcattgcat
     2461 acagcgatgt gtaacctttg tcacactcca ggcaccccgc cctgccatgc tctacacttc
     2521 ccaaacaaca ccagagaagg accaaaaaat gtcgatgata aaaagctatg ccgcaaaaga
     2581 agcgggcggc gaactggaag tttatgagta cgatcccggt gagctgaggc cacaagatgt
     2641 tgaagtgcag gtggattatt gcgggatctg ccattccgat ctatcgatga tcgacaatga
     2701 atggggattt tcacaatatc cgctggttgc cgggcatgag gtgattgggc gcgtggtggc
     2761 gctcgggagc gccgcgcagg ataaaggttt gcaggtcggc cagcgtgtcg ggattggctg
     2821 gacggcgcgt agctgtggtc actgcgacgc ctgtattagc ggtaatcaga tcaactgcga
     2881 gcaaggtgct gtgccgacga ttatgaatcg cggtggcttt gccgagaagt tgcgtgcgga
     2941 ctggcaatgg gtgattccac tgccagaaaa tattgatatc gagtccgccg ggccgctgtt
     3001 gtgcggcggt atcacggtct ttaaaccact gttgatgcac catatcactg ctaccagccg
     3061 cgttggggta attggtattg gcgggctggg gcatatcgct ataaaacttc tgcacgcaat
     3121 gggatgcgag gtgacggcct ttagttctaa tccggcgaaa gagcaggaag tactggcgat
     3181 gggtgccgat aaagtggtga atagccgcga tccgcaggca ctgaaagccc tgtcggggca
     3241 gtttgatctc attatcaata ctgtgaacgt cagcctcgac tggcagcctt attttgaggc
     3301 gctgacctat ggcggtaatt tccatacggt cggtgcggtt ctcacgccgt tgccggttcc
     3361 ggcctttacg ctgattgcgg gcgatcgcag tgtctctggc tctgctaccg gtacacctta
     3421 tgagttgcgt aagctgatgc gctttgccgc ccgcagcaag gttgcgccga ctaccgaatt
     3481 gttcccgatg tcgaaaatta acgatgccat caagcatgtg cgcgacggta aggcgcgtta
     3541 ccgcgtggtt ctgaaagctg acttctgatc tctttgccgg gtgcctgtcg cccggcactt
     3601 aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag tgcgcagccg
     3661 ttgcctatac tccaggcagg aaactggagg atatctcatg agcgaacccc tgttaattgc
     3721 ccgcacgccg gacacagaac tatttttact gccgggaatg gctaaccgtc acgggctgat
     3781 tactggcgca acggggacgg gtaaaactgt cacgctgcaa aaactggcgg agtcattgtc
     3841 ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg gtatcgcgca
     3901 ggcaggaacg gcgtcggaaa aactgctcgc aaggcttaaa aatatcgacg tcaatgactg
     3961 gcaaccgcat gctaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccattcggt
     4021 acgggcgacg gtttcagacc tggggccact gttgctggtg cggctgttga atctcaacga
     4081 tgtgcaatct ggcgtgctga atatcatctt ccgcattgct gacgatcagg ggctgttact
     4141 gctcgacttt aaagatctgc gggcgattac ccagtacatc ggcgataacg ccaaatcttt
     4201 ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc gcgggctgtt
     4261 gtcgctggaa cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga
     4321 ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg ccgagaaact
     4381 ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctctcag agttgtatga
     4441 acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaagc
     4501 acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag
     4561 gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc
     4621 ggataatgtg ctcggccagc tcggtaatcg cgttcagcac gctttgcggg cttttacgcc
     4681 caaagatcag aaagcagtaa aagctgcggc gcaaaccatg cgggccaatc cggcgtttga
     4741 taccgaaaag gcgattcagg aactgggcac cggcgaggcg ttgatctctt ttctggatgc
     4801 gaaaggaagc ccttctgtgg tggagcgtgc gatggtgatc gcgccttgtt cgcggatggg
     4861 gccggtgacg gaagatgagc gtaatggctt gattaatcac tctccggtgt atggcaaata
     4921 tgaggatgag gtggaccggg aatccgccta tgagatgttg caaaaaggct ttcaggccag
     4981 taccgagcag caaaataatc cccccgcgaa agggaaagag gtagcggtgg atgacggcat
     5041 tcttggtgga ttgaaggata ttttgttcgg cactaccgga ccacgcggcg ggaagaaaga
     5101 tggtgtggtg caaacaatgg ccaaaagcgc cgctcgccaa gtgacgaatc agattgtacg
     5161 cgggatgttg gggagtttgc tgggggggag aagaaggtaa gtcaggcgag atttgtcttt
     5221 gcctgatgcg acgctggcgc gtcttatcat gcccacccca ctgcaatata ttgaatttta
     5281 attatttttc aggaatgaac gaagcacact ttgctaacaa taataaaggg agctttcgct
     5341 ccctttattc gttcattcgg ttacgatttt ctcattaaca gccacaggct gattaagaag
     5401 aagctggcgc ttggcaacag tgcgccgatg atcggcggga tgccataaac caacgtcagc
     5461 ggaccgaaga tctggtccag tacgtagaag acaaaaccga aactgatacc ggtgaccaca
     5521 cgcacgccca tcggtacgct acgcagtggg ccaaagatga acgacagcgc catcagcatc
     5581 atcaccgcca cggatagcgg ctggaagatt ttgctccaca tgttgagctg ataacgtccg
     5641 gcatcctgac cgctcgactt cagatacttc acatagttgt gcaaaccgct gatagagagt
     5701 gcatccgggt ccagcgccac cacgcccagt ttgtccggcg tgaggttggt tttccaggtg
     5761 ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg tcagatcaga ttcatcaacc
     5821 tgcgacaaac gccagacttt atgttccggg tcaaacttcg cagtagcggc atagcgtacg
     5881 gattgcagac gacgattctc gttaaaggca taaatgctga tgccacctaa ctcttcgtca
     5941 cctttaaccc gctcaatgta gacgaagttg ttgccatctt tcgcccataa gccttgctgg
     6001 gtagagagca acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc catctgctcg
     6061 ccctgcggcg cgacccattc gccaatcgcc atcgtcagca agaccagcgg aatggcggtt
     6121 ttcatcaccg acagcgccac ctgcatacgg gtaaaaccag aagcctgcat caccaccagt
     6181 tcgctgcgct gcgccagcat cccaagacca agcaacgccc caagcagagc cgccatcggg
     6241 aagaatattt gcacatcttt cggcacgctc agcaaggtat acattcctgc gcctaacgcg
     6301 tcgtaactcc cctgcccggc ttttttcaac tgatcgacaa acttgataat gcccgacagc
     6361 gacaccagca tgaacagcgt catcatgatg gtggtgaaaa tagttttacc gatatagcgg
     6421 tcaagtacgc caaaaggttg catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc
     6481 ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc caaagccaga taaatcaggt
     6541 taacggtcca catccacagc gtcgggtcca gcttaccttt accgccgttc gatttcaggg
     6601 aggtctggat caggaagaaa agtagataca gcagcatggc tggcagcatc gacagtacgc
     6661 gtccctgacg tgggttaacc acgctcagcg gtacgaccat aagtgccatc ataaacacgg
     6721 tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc
     6781 acaatgtgcg catgtccatc tggtcggtat cgttcgggtc gagcgccacc gcctggtgac
     6841 caatgatcgc ctgataatcc tggaaatccg taatgcggaa atcacgtaac agtgcagtgc
     6901 cttcgaagcg cgttccctgg ttgagagtga cgacctggga gccgtcgcgc agctgggtta
     6961 aatgtccgga atcggccacc accacagaag gacgtgcatt accttttggt cgaatttgcg
     7021 cgaggaacac atctttgaaa tcgctgccgt caacgctttc gatgaacagc accgaactgc
     7081 cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg
     7141 cttctgctaa cacttcatcc tgatgacgcg atgaccacgg tcccgcccac atcacgttaa
     7201 ccgccgcgac gattgccgtg aataccgcaa ggatcattgc cgctttcacc agaaccgctt
     7261 tgctcaggcc gcaggcatgc attaccgtaa tttcactttc ggtatacagt ttgcccagcg
     7321 tcatcagcag cccgaggaac aggcttaatg gcaggataag ctgcgccatt tccggcacgc
     7381 ccaacccgag aagggagagc accagattcg ccggaatatc gccgtcaacc gctgcgccga
     7441 ggatcctcac taacttttga cagaagaaga tcaaaagcaa gatgaagagt atcgccagct
     7501 ggcttttgag cgtctcccgc accagatatc ttatgattat cactttaaat acgcccgtaa
     7561 aaactcgtct tttgcaggat tttagcttgt ttcatggctt aaacgtcatt tattctcttg
     7621 agtcgtcgaa atcgtcgcta agataattat actcaacgga ttcacctctc agattttgtt
     7681 ctgacgtgcc aatgccgtaa taacgttaag attaacacga agtcatcgca acagcggaca
     7741 tgagttacga aagcttgcaa ttctatctgt agccaccgcc gttgtcttta agattcagga
     7801 gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc
     7861 atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga ttgcagaaca gctcgataaa
     7921 atcagcgatg ggtacatcag cgccctgcta cgtcggggcg aactggaagg aaaaccgggg
     7981 cagacattgt tgctgcacca tgttccgaat gtactttccg agcgaattct ccttattggt
     8041 tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc aggttattca gaaaaccatt
     8101 aatacgctga atgatactgg ctcaatggaa gcggtctgct ttctgactga actgcacgtt
     8161 aaaggccgta acaactactg gaaagtgcgt caggctgtcg agacggcaaa agagacgctc
     8221 tacagtttcg atcagctgaa aacgaacaag agcgaaccgc gtcgtccgct gcgtaagatg
     8281 gtgttcaacg tgccgacccg ccgtgaactg accagcggtg agcgcgcgat ccagcacggt
     8341 ctggcgattg ccgccgggat taaagccgcg aaagatctcg gtaatatgcc gccgaatatc
     8401 tgtaacgccg cttacctcgc ttcacaagcg cgccagctgg ctgacagcta cagcaagaat
     8461 gttatcaccc gcgttatcgg cgaacagcag atgaaagagc tggggatgca ttcctatctg
     8521 gcggtcggtc agggttcgca aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac
     8581 gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt cgactccggc
     8641 ggtatctcga tcaagccttc agaaggcatg gatgagatga agtacgatat gtgcggtgcg
     8701 gcagcggttt acggcgtgat gcggatggtc gcggagctac aactgccgat taacgttatc
     8761 ggcgtgttgg caggctgcga aaacatgcct ggcggacgag cctatcgtcc gggcgatgtg
     8821 ttaaccacca tgtccggtca aaccgttgaa gtgctgaata ccgacgctga aggccgcctg
     8881 gtactgtgcg acgtgttaac ttacgttgag cgttttgagc cggaagcggt gattgacgtg
     8941 gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc acattaccgg tctgatggcg
     9001 aaccataatc cgctggccca tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca
     9061 tggcgcttac cgctgggtga cgagtatcag gaacaactgg agtccaattt tgccgatatg
     9121 gcgaacattg gcggtcgtcc tggtggggcg attaccgcag gttgcttcct gtcacgcttt
     9181 acccgtaagt acaactgggc gcacctggat attgcaggaa ccgcctggcg ttctggtaaa
     9241 gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac agttcctgtt gaaccgcgct
     9301 gggtttaacg gcgaagagta attgcgtcag gcaaggctgt tattgccgga tgcggcgtga
     9361 acgccttatc cgacctacac agcactgaac tcgtaggcct gataagacgc gcaagcgtcg
     9421 catcaggcaa tgctgttatt gtcggatgcg gcatgaacgc cttatccgac ctacacagca
     9481 ccgaactcgt aggcctgata agacgcggca gcgtcgcatc aggcaatgct gttattgtcg
     9541 gatgcggcgt gagcgcctta tccgacctac acacagcact gaactcgtag gcctgataag
     9601 acacaacagc gtcgcatcag gcgctgcggt gtatacctga tgcgtattta aatccaccac
     9661 aagaagcccc atatatgaaa aacgcgacgt tctaccttct ggacaatgac accaccgtcg
     9721 atggcttaag cgccgttgag caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg
     9781 gtaagcgcgt gctcatcgcc tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc
     9841 tgtgggcgcg tccggcagaa agctttgttc cgcataattt agcgggagaa ggaccgcgcg
     9901 gcggtgcgcc ggtggagatc gcctggccgc aaaaacgcag cagcagcccg cgcgatatat
     9961 tgattagtct gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa gtggtagact
    10021 tcgttcctta tgaagattct ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg
    10081 tggctggttt caacctgaat acggcaacct ggaaataatg gaaaagacat ataacccaca
    10141 agatatcgaa cagccgcttt acgagcactg ggaaaagcag ggctacttta agcctaatgg
    10201 cgatgaaagc caggaaagtt tctgcatcat gatcccgccg ccgaacgtca ccggcagttt
    10261 gcatatgggt cacgccttcc agcaaaccat catggatacc atgatccgct atcagcgcat
    10321 gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac gccgggatcg ctacccagat
    10381 ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact acggccgcga
    10441 agctttcatc gacaaaatct gggaatggaa agcggaatcc ggcggcacga ttacccgtca
    10501 gatgcgccgt ctcggcaact ccgtcgactg ggaacgtgaa cgcttcacga tggacgaagg
    10561 cctgtccaat gcggtgaaag aagttttcgt tcgtctatat aaagaagacc tgatttaccg
    10621 tggcaaacgc ctggtaaact gggacccgaa actgcgcacc gctatctctg acctggaagt
    10681 ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg ctgacggtgc
    10741 gaaaaccgca gacggtaaag attatctggt ggtcgcgact acccgtccgg aaaccctgct
    10801 gggcgatact ggcgtagccg ttaacccgga agatccgcgt tacaaagatc tgattggcaa
    10861 atatgtcatt ctgccgctgg ttaaccgtcg tattccgatc gttggcgacg aacacgccga
    10921 catggaaaaa ggcaccggct gcgtgaaaat cactccggcg cacgacttta acgactatga
    10981 agtgggtaaa cgtcacgcgc tgccgatgat caacatcctg acctttgacg gcgatatccg
    11041 tgaaagcgcc caggtgttcg ataccaaagg taacgaatct gacgtttatt ccagcgaaat
    11101 cccggcagag ttccagaaac tcgagcgttt tgctgcacgt aaagcggtcg ttgctgcggt
    11161 tgacgcactc ggcctgctgg aagaaattaa accgcacgac ctgacagttc cttacggcga
    11221 ccgtggcggc gtagttatcg aaccaatgct gaccgaccag tggtacgtac gtgccgatgt
    11281 actggcgaag ccggcagttg aagcggttga gaacggcgac attcagttcg taccgaagca
    11341 gtacgaaaac atgtacttct cctggatgcg cgatattcag gactggtgta tctctcgtca
    11401 gctgtggtgg ggtcaccgta tcccggcatg gtacgacgaa gcgggtaacg tttatgttgg
    11461 ccgcaacgaa gaagaagtgc gcaaggaaaa taacctcggt gccgacgttg ccctgcgtca
    11521 ggacgaagac gttctcgaca cctggttctc ttctgcgctg tggaccttct ctaccctagg
    11581 ctggccggaa aacaccgacg cactgcgcca gttccaccca accagcgtga tggtatctgg
    11641 tttcgacatc atcttcttct ggattgcccg catgatcatg atgaccatgc acttcatcaa
    11701 agatgaaaat ggcaaaccgc aggtgccgtt ccacaccgtt tacatgaccg gtctgattcg
    11761 tgacgacgaa ggccagaaga tgtccaaatc caagggtaac gttatcgacc cgctggatat
    11821 ggttgacggt atttcgctgc cagaactgct ggaaaaacgt accggcaata tgatgcagcc
    11881 gcagctggcg gacaaaatcc gtaagcgcac cgagaagcag ttcccgaacg gtattgagcc
    11941 gcacggcacc gatgccctgc gcttcaccct ggcggcactg gcgtctaccg gtcgtgacat
    12001 caactgggat atgaagcgtc tggaaggcta ccgtaacttc tgtaacaagc tgtggaatgc
    12061 cagccgcttt gtgctgatga acacggaagg tcaggattgc ggcttcaacg gcggcgaaat
    12121 gacgctgtcg ctggcggacc gctggattct ggcggagttc aaccagacca tcaaagcgta
    12181 ccgcgaagcg ctggacagct tccgcttcga tatcgccgca ggcattctgt atgagtttac
    12241 ctggaaccag ttctgtgact ggtatctcga gctgaccaag ccggtaatga acggtggcac
    12301 cgaagcagaa ctgcgcggta ctcgccatac gctggtgact gtactggaag gtctgctgcg
    12361 cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact
    12421 ttgcggtatc actgccgaca ccatcatgct gcagccgttc ccgcagtacg atgcatctca
    12481 ggttgatgaa gccgcactgg ccgacaccga atggctgaaa caggcgatcg ttgcggtacg
    12541 taacatccgt gcagaaatga acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg
    12601 ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgt ggcttcctgc aaaccctggc
    12661 gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa ggtccggttt ccgttacgaa
    12721 gatcatcgac ggcgcagagc tgctgatccc gatggctggc ctcatcaaca aagaagatga
    12781 gctggcgcgt ctggcgaaag aagtggcgaa gatcgaaggc gaaatcagcc gtatcgagaa
    12841 caagctggcg aatgaaggct ttgtcgcccg cgcaccggaa gcggtcatcg cgaaagagcg
    12901 tgagaagctg gaaggctatg cggaagcgaa agcgaagctg attgaacagc aggctgttat
    12961 cgccgcgctg taatcaaaac acagtgatga aaacgaaggc cggagcatgc tccggccttt
    13021 ttatctctta cagaaatggt aaagaaggca taattccccg tgaaatactg gctaagaagc
    13081 ctttatctgg ttgttcttct ttattgatta aaggaaggtc atccaaatcg cccagcaaat
    13141 acgtattttt tgcctgccag tcaatcatcc atattttcag cagtggataa gccagaccgc
    13201 ccgtaatccc ggatatcacc accaacgcac acatgcgata aagcataccg tggtatgtta
    13261 aagttgagcg aaaacgaata cgcccatcat tcagtgacag gttgctcata aaatgatttc
    13321 gcaacgacac cgttaaataa ctggtgctaa cagcaatccc aaaataatag attaactgcg
    13381 cgattatcat tttacgttgc atttccataa attgctgtaa attctcaata tcatcatttg
    13441 catacacaga actatcatac gcatttaata tttgatcgaa gataatataa ccagcaacag
    13501 cgagaaaagg taataaagct aaaaatgcga ggatggcata ttttatacaa tacgcagtat
    13561 ctaatttcac cttgaaacga tgtataccga aactggtatt gctccagaga aaactcatta
    13621 ccaggctata taaagtacca ttaaaaatac caatggaaac aataccaact attgccatca
    13681 gaaccacaga tacaataaca ctacttgaac tattggcatg tagcatcttt gtcgagataa
    13741 agaaaacagt ccccatccca atggccatta aaatcggcaa gaaaaaggtt acccaccaga
    13801 cccctttcat agagcaatta aaactaaatc ttacgccgtt gagactggtc atcaaggcct
    13861 gataacgcag tccttttgct gccataaata taagcaaaac caacagtgac aaagtcaaaa
    13921 cacagccaat aagcggcata tctgctgaca ctgtcattaa gattgcgaaa tagaaaaaaa
    13981 caaaaacaag acaactaaaa aaaacattcc caccagtaat tccataagaa aatcgttgtc
    14041 cgttaacttc catattagca tagagataac gcttacattt cattaatgcc catggtaaat
    14101 agatacctag cgttataatc gttaacaaca cattcacc
//
LOCUS       DBFIFS010000117        13973 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.117, whole genome shotgun sequence.
ACCESSION   DBFIFS010000117 DBFIFS010000000
VERSION     DBFIFS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.117"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..2683
                     /gene="stcE"
                     /locus_tag="N7A07_003007"
     CDS             <1..2683
                     /gene="stcE"
                     /locus_tag="N7A07_003007"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HGU2477630.1"
                     /translation="NERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYFNTSQP
                     INDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTPVQVEA
                     RDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVNKLSDA
                     SGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTVFYGDR
                     KVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLVIKQGN
                     LSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSRMIVNN
                     YAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLNSTAGL
                     GENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGHNYGLG
                     HYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPFDGHKF
                     GFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADTQEMEP
                     YEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASADNRGS
                     ILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPEQFGVP
                     VTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQLRFRLA
                     NHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQALPAK
                     ENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDNLSYNR
                     IGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2770..3645
                     /gene="gspC"
                     /locus_tag="N7A07_003008"
     CDS             2770..3645
                     /gene="gspC"
                     /locus_tag="N7A07_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGU2477631.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3646..5613
                     /gene="etpD"
                     /locus_tag="N7A07_003009"
     CDS             3646..5613
                     /gene="etpD"
                     /locus_tag="N7A07_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HGU2477632.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5613..7118
                     /gene="gspE"
                     /locus_tag="N7A07_003010"
     CDS             5613..7118
                     /gene="gspE"
                     /locus_tag="N7A07_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGU2477633.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7120..8343
                     /gene="gspF"
                     /locus_tag="N7A07_003011"
     CDS             7120..8343
                     /gene="gspF"
                     /locus_tag="N7A07_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGU2477634.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8374..8808
                     /gene="gspG"
                     /locus_tag="N7A07_003012"
     CDS             8374..8808
                     /gene="gspG"
                     /locus_tag="N7A07_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGU2477635.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8805..9359
                     /gene="gspH"
                     /locus_tag="N7A07_003013"
     CDS             8805..9359
                     /gene="gspH"
                     /locus_tag="N7A07_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGU2477636.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9356..9721
                     /gene="gspI"
                     /locus_tag="N7A07_003014"
     CDS             9356..9721
                     /gene="gspI"
                     /locus_tag="N7A07_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGU2477637.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9718..10317
                     /gene="gspJ"
                     /locus_tag="N7A07_003015"
     CDS             9718..10317
                     /gene="gspJ"
                     /locus_tag="N7A07_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGU2477638.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10314..11291
                     /gene="gspK"
                     /locus_tag="N7A07_003016"
     CDS             10314..11291
                     /gene="gspK"
                     /locus_tag="N7A07_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGU2477639.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11225..12502
                     /gene="gspL"
                     /locus_tag="N7A07_003017"
     CDS             11225..12502
                     /gene="gspL"
                     /locus_tag="N7A07_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGU2477640.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12489..13001
                     /gene="gspM"
                     /locus_tag="N7A07_003018"
     CDS             12489..13001
                     /gene="gspM"
                     /locus_tag="N7A07_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HGU2477641.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13122..13892
                     /locus_tag="N7A07_003019"
     CDS             13122..13892
                     /locus_tag="N7A07_003019"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU2477642.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
BASE COUNT         3336 a         3099 c         4047 g         3491 t
ORIGIN      
        1 gaatgagaga tggagaacac cgatgaaatt aaagtatctg tcatgtacga tccttgcccc
       61 tctggcgatt ggggtatttt ctgcaacagc tgctgataat aattcagcca tttatttcaa
      121 tacctcccag cctataaatg atctgcaggg ttcgttggcc gcagaggtga aatttgcaca
      181 aagccagatt ttacccgccc atcctaaaga aggggatagt caaccacatc tgaccagcct
      241 gcggaaaagt ctgctgcttg tccgtccggt gaaagctgat gataaaacac ctgttcaggt
      301 ggaagcccgc gatgataata ataaaattct cggtacgtta accctttatc ctccttcatc
      361 actaccggat acaatctacc atctggatgg tgttccggaa ggtggtatcg atttcacacc
      421 tcataatgga acgaaaaaga tcattaatac ggtggctgaa gtaaacaaac tcagtgatgc
      481 cagcgggagt tctattcata gccatctaac aaataatgca ctggtggaga tccatactgc
      541 aaatggtcgt tgggtaagag acatttatct gccgcaggga cccgaccttg aaggtaagat
      601 ggttcgcttt gtttcgtctg caggctatag ttcaacggtt ttttatggtg atcgaaaagt
      661 cacactctcg gtgggtaaca ctcttctgtt caaatatgta aatggtcagt ggttccgctc
      721 cggtgaactg gagaataatc gaatcactta tgctcagcat atttggagtg ctgaactgcc
      781 tgcgcactgg atcgtgcctg gtttaaactt ggtgattaaa cagggcaatc tgagcggtcg
      841 cctaaatgat atcaagattg gagcaccggg tgagctgttg ttgcatacaa ttgatatcgg
      901 gatgttgacc actccccggg atcgctttga ttttgccaaa gacaaagaag cacataggga
      961 atatttccag accattcctg taagtcgtat gattgttaat aattatgcgc ctctacacct
     1021 aaaggaagtt atgttaccaa ccggagagtt attgacagat atggatccag gaaatggtgg
     1081 gtggcatagt ggtacaatgc gtcaaagaat aggtaaagaa ttggtttcgc atggcattga
     1141 taatgctaac tatggtttaa atagtaccgc aggcttaggg gagaatagtc atccatatgt
     1201 agttgcgcaa ttagcggcac ataatagccg cggtaattat gctaatggca tccaggttca
     1261 tggtggctcc ggaggtgggg gaattgttac tttagattcc acattgggga atgagttcag
     1321 tcatgaagtt ggtcataatt atggtcttgg tcattatgta gatggtttca agggttctgt
     1381 acatcgtagt gcagaaaata acaactcaac ttggggatgg gatggtgata aaaaacggtt
     1441 tattcctaac ttttatccgt ctcaaacaaa tgaaaagagt tgtctgaata atcagtgtca
     1501 agaaccgttt gatggacaca aatttggttt tgacgccatg gcgggaggca gccctttctc
     1561 tgctgcaaac cgtttcacaa tgtatactcc gaattcatcg gctatcatcc agcgtttttt
     1621 tgaaaataaa gctgtgttcg atagccgttc ctccaccggc ttcagcaagt ggaatgcaga
     1681 tacgcaggaa atggaaccgt atgaacacac cattgaccgt gcggagcaga ttacggcttc
     1741 agtcaatgag ctaagtgaaa gcaaaatggc tgagctgatg gcagagtacg ctgtcgtcaa
     1801 agtgcatatg tggaacggta actggacaag aaacatctat atccctacag cctccgcaga
     1861 taatagaggc agtatcctga ccatcaacca tgaggccggt tataatagtt atctgtttat
     1921 aaatggtgac gaaaaggtcg tttcccaggg gtataaaaag agctttgttt ccgatggtca
     1981 gttctggaaa gaacgtgatg tggttgatac tcgtgaagcg cgtaagccag agcagtttgg
     2041 tgttcctgtg acgaccctgg tggggtatta cgatccggaa ggcacgctgt caagctacat
     2101 ctatcctgcg atgtatggtg cctatggctt cacttattcc gatgatagtc agaatctatc
     2161 cgataacgac tgccagctgc aggtggatac gaaagaaggg cagttgcgat tcagactggc
     2221 taatcaccgg gctaacaaca ctgtaatgaa taagttccat attaacgtgc caacagaaag
     2281 tcagcccaca caggccacat tggtttgcaa taacaagata ctggatacca aatcgctcac
     2341 acctgcgcca gaaggactta cctatactgt aaatgggcag gcacttccag caaaagaaaa
     2401 cgagggatgc atcgtgtccg tgaattcagg taaacgttac tgtttgccgg ttggtcaacg
     2461 gtcaggatat agccttcctg actggattgt tgggcaggaa gtctatgtcg acagcggggc
     2521 taaagcgaaa gtgctgcttt ctgactggga taacctgtcc tataacagga ttggtgagtt
     2581 tgtaggtaat gtgaacccag ctgatatgaa aaaagttaaa gcctggaacg gacagtattt
     2641 ggacttcagt aaacctaggt caatgagggt tgtatataaa taatcctgag acggtgcggc
     2701 cacttcggcc gcattagtaa cacattcgtc ccgttaatca aaacataaag tagcgtccag
     2761 cgttttatta tgttgttctt tctatcattc cggggtgacc ggggcttatt tataaaagat
     2821 atcgtactta aaatgctgac gccaaaccgg ctattgtgcg taattttact tattgccgga
     2881 tatcagctgg tgtcggttat ccatcatttc tggctgactc aggcagcatc ggtgcccggc
     2941 ttatcccgtg ttagcgcgcc ggaaacagcg gtaactggtg atcaaactga agaacgtttt
     3001 gtttttacgt tattcggcag ggcatcccca ctatcatcgg aggggagagc gcaggaaaca
     3061 atgccttccc tgtcagatga tctgctttca ggggaggatc ttgacgtgag aggtatactt
     3121 tatagttcgg ttgcagagca ttccgttgcc atatttgcac ataataacag acagttcagt
     3181 ctgagcgtcg gcgaaaaagt acccagctac gatgctacga ttagtgctat ttttagcgat
     3241 catattgtta ttaactatca gggaaaaact gtatcactgc ctctgcgata tgataatacc
     3301 gaaaaaaaga atgcatatga caataataat ttaacagttg gagacgtgat aacccaagat
     3361 aattttcggg tagaaagtgt ttttgatatt atgagctttt cagccgttac ggttaataat
     3421 acattaagcg gttatcgctt gattccgggt aagcacagtt cgttatttta taatgctggg
     3481 ttgcatgata acgatctggc cgtatcggtt aatggttcag aattgcgtga taccagacag
     3541 gcgcagcaga taatgaagca attgccagaa cttaaagaaa taaaaataac cgtcgagcgt
     3601 gatggtcagt tatatgatgc atttattgct gtaggagaaa actgattgtt caggaaatgg
     3661 ttgaatgggc gtttgccggt acttgtgttc actacagtaa ttttgggggc cattccaggg
     3721 tggggggctg aattttcggc caactttaaa gatacggata ttcaggagtt cataaatact
     3781 gtcagtaaaa atttacacaa aacggtaata attaatcctg acgtgcaggg aaccatcact
     3841 gtacgcagct acgatatgct gaacgaggaa caatattatc agttctttct cagtgtgctg
     3901 gacgtttatg gttttgctgt ggtcgatatg cacaacggta tactgaaagt agtgcgctca
     3961 aaagatgcca aaacgtcggc ggtgccggta gctagtgatg tcagtcccgg gactggtgat
     4021 gaggttgtta cccgggtggt ccccgtaagt aacgtggcag ccagagatct ggcgcctttg
     4081 ctgcgtcagc tcaatgataa tgctggcgca ggaagcgtgg tgcattatga accttctaat
     4141 gttttgttga tgaccggacg tgctgcagtg atgaaacggt tgatggagat tgttgaacgt
     4201 gtggataagg tgggtaatcg cagcgttgcc acggtcccgc tcacctacgc gtccgcaaca
     4261 gacgtagcca gacttgttac ggaactgact aaagaaacag ataagacagc tatacctgct
     4321 tggatgacgg cgaaactggt tgcagacgag aggacaaact cagtgctcgt cagcggagag
     4381 ccaatctccc aacagcgtat catctccata attaagcaac tggatcgtca ggaggatgtt
     4441 cagggtaata ctaaggtgat ttacctgaaa tatgcgaagg cgaaggattt agtggaagtc
     4501 ctgacaggta tcagcagcag tattgaaaac gactctaaaa agagtccgtc aacggaagcc
     4561 ttgcgcaaag gagtgacgat taaatcccac gaacaaacca atgccctgat cctgacgggg
     4621 gcccctgacg tcatccgcga ccttgaaaat gtgatttcgc agttggatat tcgtcgtcct
     4681 caggtcctgg tggaggccat cattgctgaa atacaggatg ctgacgggct gaaccttggg
     4741 atccagtggg tgaataaaca tgccggtgtg gcgcagttca ccagtaccgg tttacctatt
     4801 accacgatgg ttcagactcg tcagaacgaa atcttagaca gcgatcagag caatgccctg
     4861 agcatgttta acggaattgc agcggggttt tatcagggaa actgggcgat gctgttgacg
     4921 gcgctctcca caagtagcaa gaatgatatc ttggcgaccc ccagtattgt cacgctggac
     4981 aatatggagg ccactttcaa tgttggtcag gaggtcccgg tactttcggg ctcacagaca
     5041 acctctgggg acaatatttt taacacggtc gagcgcaaaa cggtggggat caaactcagg
     5101 gtaaaacccc agatcaacga gggtgattcc gtgttactgg agatagaaca ggaggtgtcc
     5161 ggtgtggcgg acactgcagt agccaccact actgacttgg gagcaacctt caacacccga
     5221 acagtgacca atgccatgct ggtcgggaat ggcgaaacgg tggtggtcgg aggattactg
     5281 gataagtcga tcagggggag tgagagtaaa gtgccactgc tgggggatat cccggtactg
     5341 gggcatcttt ttcgcgcaaa aagcgaacag acagctaagc gtaatctgat gctgttcatt
     5401 cggccaacta ttattcgtga gcgcgacgga tttcgtcatg cttcggccga aaaataccag
     5461 tcgtttaatc aggaacaggt gcagtcgcgt ggcaaagaaa caacggcgct gacgctgaat
     5521 gaggaacagc tcaggctgtc ccccgatcaa gacgatacgg ctttccggaa ggtgaaagcg
     5581 gcgattgctg cgttttatgc gcaggagatg taatgagcag agtagtacag aatgtcagtg
     5641 aatcacgtcc ccttctgcct ttttcctttt cccgtacgca gagaattttg ttgctgcgtg
     5701 aacaggaggg gaatcgagtg ttctgcatgg aggacacacc tgccagtgcc ctgttggagg
     5761 tgcgcagggt ggctgaaggt cctctgaatg tcacaacagt gtcggcggaa gcgtttgaaa
     5821 aacagctggt gagcagctat caacgtgatt ctgatgaagc gcgacaaatg atggctgaga
     5881 ttggtaatga aatggatttt tataccgtcg caggggagtt gcctgaccgt gaggacttac
     5941 tggatgcgaa tgacgatgcg ccaattatcc gcctgattaa cgcaatgctg acagaggcaa
     6001 ttaaagagaa agcctcagat attcatattg aaacttacga acgccatctg caggttcgct
     6061 ttcgcattga tggcgttctg cgggagatcc tccgtttaca taggaatctg gcttcgttac
     6121 tgatttcacg cattaaagtc atggcccggt tggatattgc cgaaaaacgc gttccccaag
     6181 atggccgcat ggtactgcgt atcggtggtc gggcggtgga tgtgcgtgtt tcaacgttgc
     6241 cttcaaatca tggtgaacgc atcgtgctgc gtttactaga caaaaatagc gttagtctcg
     6301 atcttgctgc actcggcatg tcgcagcaga atcagcgaca cattgatgca ctgatccgcc
     6361 gtcctcacgg cattattttg gtgaccgggc ctacagggtc gggtaaaagc acgacgcttt
     6421 atgccgcgtt aagcctgctg aatccccgag accgcaacat tatgacggtc gaggatcctg
     6481 ttgaatatga actggacggt atcagtcaga cccaggtcaa cccgaaggtg gacatgacct
     6541 ttgcccggag tctgcgcgct attttacgtc aggatccgga cgtggtgctt gtgggggaga
     6601 tccgtgacgg tgaaacagcc cagattgctg tgcaggcctc gctgacaggg catttggtgc
     6661 tgtcaacgtt acatacgaat agtgcagcag gcgcgctgtc gcgtctgcag gatatgggca
     6721 tccctccttt tctgctttcc acctcattac tggctgttct ggcccagcga ctggttcgca
     6781 cgttgtgtcc gcgctgtcgt cagccctgtc aggtcagtac tgagttagcg atggacatgg
     6841 acattccccc tgaaaccacg atctggcagc ctgccgggtg tcagcactgc agtttcacgg
     6901 gctatcacgg gcgcaccggg atccacgagc tgttgctgat tgacgatcgc attcggacgg
     6961 caatctatca gggggagggg gagctgggta ttacccgctt ggcagggagt cgctatctga
     7021 cgctgcgtgg cgacgggcga caaaaggtac tagccggtga gaccagttgg gaggaggtgg
     7081 ttcgcgttac tgaaagcaga ttgcaggaag aggaatgata tggcgttgtt tcattatcag
     7141 gcatcagata ttcacggtag aaaacgcagt ggcattctgg aggcggattc ggcccgacat
     7201 gcccgtcagt tgctgcgtga gcaggcgctt attccagtca ggttggatga gaaacaggtc
     7261 catcacaagc actcactgcg gagtatcctg aggtttcgtc cgcgcggggg gagcagcgcc
     7321 gaactcgcgt tactgacacg gcagttagcc acactagtgg ccgcatctct gccgcttgaa
     7381 gaggcgctgg atgcgttgtt gaggcagagt gaaaaaccgc gtcagcggaa cttaatcgct
     7441 gccgtgcgca ccaaggttct cgagggacat tccttggccg cggcgatggg catgtttcca
     7501 ggtacgtttg agcgtcttta ttgtgcgatg gtggcggcgg gggaaacatc cggtcgtttg
     7561 gacgtggtgc tcagtcgtct ggctgactat accgagcagc gccagatcat gcgaaaccgt
     7621 ctactccagg cattgctcta tccttgtgtt ctgacgttgg tggcggttgg ggttattgcc
     7681 attctgctta ctgcggttgt gccgaaagtg gttgagcagt ttattcatat gaaacagacc
     7741 cttcccttat ctacccgcgt actgatgggg gctgctgagg tgagccagac ttggggcccg
     7801 tggctgttac tggccgcagc gctgggcggg atagcggggc gcatgatatt acatcagccc
     7861 tcccagcgtc tggcttttca tcatctgctg ttgcggctgc cggtcgtggg gcgcatatcg
     7921 cgcggtctga ataccgcacg ttatgcccgc acgctgagca ttcttaatgc cagtgcggta
     7981 ccgctgctcc aggcgatgca catcagtggt gacgtactga gtaatgactg ggcccgtcat
     8041 cagttagcta ccgcggccga gttggttagg gaaggggtca gcctgcatca ggcactggag
     8101 cagacttcgc tgtttccgcc tatgatgcgg cacatgattg ccagcggtga aaatagtggc
     8161 gaactcgaca gcatgttgga acgggccgcc gacaatcagg atcgtgagtt cagcacacag
     8221 atgcaactgg cgctgggatt gtttgaaccg ctgttggtgg tgggtatggc cggggtcgtt
     8281 ttgtttattg ttctggcaat tctgcagccg ctcctgcagc tcaacaacat gatgaatatg
     8341 tgaatccttc tttaacagtc aacggagaaa aacatgcgaa aacagcatca gcgtggtttt
     8401 accttgctgg aaataatggt ggtaattgtg atcttgggag ttttggccag tctggtggtc
     8461 ccgaatctga tgggaaataa ggataaggcc gatcggcaga aagtgatgag cgacttggtt
     8521 gcgctggaaa gcacgctcga tatgtaccgg ttagataata accggtatcc gacaacggag
     8581 cagggactgc gggcactggt cagtaaaccc accgtgcaac ctgaaccccg taactaccgg
     8641 caggatggtt atatccgccg tttgccccag gatccttggg ggggggatta tcagctgtta
     8701 aatcctggtc agtacagcga cattgatatc ttttctcctg gcccggatgg tgtgccaaac
     8761 actgaagatg atattggtaa ctggacgctc ggtaacgcgc agccatgagt cagcgcggtt
     8821 tcacaatact ggagatgatg ctgatccttt tgttgatggg catcactgca gggctggtac
     8881 taatgtcatt tcctgactct gctcagaatc atctgcaaca acagcgggag cgtctgcagg
     8941 cgcagctgga ttatgccttg gatagaagcc agcaggacgg cttgctgatg gggattcagg
     9001 ttcggtttga cggatggaaa tttaaggttc tgcagcgcgg aacagcggaa agccccccga
     9061 cattagctga gggaggggat atttggcagg ggtatgtctg gcaaacttgg caaccgcgac
     9121 gtgcagccat ggggggcaaa cttccggatg atgtgcgtct tgagctgcag tatctcagag
     9181 gtttgcagtg gatgtctgag catgatgacg gggctgaacc cgatattttg ctgcttcccg
     9241 gaggtgaggt aacgccgttc agacttcttt tcaggcaggt cggagaggag gccgttgtcg
     9301 ggctgcaggt tgatgagaac ggtttgatga ctctttttga aggtgaggtc tcattgtgaa
     9361 ggggcaagaa ggaatgacat tactggaagt catagtagct ctggttgtgt ttgcgctggc
     9421 aggaatggcg ctgatgcagg cctcgaccca gcaggctgcg gggatcggac ggatggaaga
     9481 gaaggtgctg gcaggctggt tggccgataa ccagatggtg caattgcaac tcgagaaaac
     9541 ttggccggaa aatggctggg gcgagaagac gatcagcttc gctggcaccg aatggtattt
     9601 acgctggggc gggccggaca gcgacgtgcc gcagccgcgc tccttagagg tagaggtacg
     9661 gcgcactaaa gaggagacgt ccgccttggt ttccttgcgt agcagtgtgg ttcgtgaatg
     9721 agtcagcaac gggtaaaagg ttttacgcta ctggaaatgc tgttggcact ggctgttttt
     9781 gcagcgttga gcatcagcgc atttcaggtg ctgcagggtg gaatacgggc gcacgagctg
     9841 tcccgggata aagtacagcg tctggctgag ctgcagagag ggatcagcca gatggagcga
     9901 gatcttacgc agatgctccc aaggcacagc cgggggaatg aaggcctgct gctcgctgca
     9961 cctcatctgc taaaaagtga tgactggggg atttctttta cgcgaaacag ctggctgaat
    10021 cccgccggga tgctgccgcg cccggagtta cagtgggtag gttatcggtt gcgacagcaa
    10081 aagttagaac gcctgagcta ctttcatgtc gatcaccctt ccggtgtttc tccggatgtc
    10141 agggtgatgc tggacggcgt tcacgcattt cgtctgcgtt tttttgttaa cggtgattgg
    10201 caggcgcgct gggacagtac cggcatcctg ccgcaggctg ttgaagtgac gctggttatg
    10261 gatgattttg ctgaactgcc ccgtctgttt cttgtgagca aggagaccgc tgaatgaaat
    10321 tgcgggaaca gggcgtggcg cttctggtgg tattgctgat cctgtcgtta atggtaacga
    10381 tagcggcggt gattgcggag cgcaacggtc ggacattttt gcgtacagta gcgcagttgg
    10441 atcagctgca ggcaaagtgg gatggctata cggcagagac gatagcaaaa caaatcttac
    10501 agaggagcag gcaggagtcg ccccggaaaa cgcatctggc acaaaattgg gcgcagtcag
    10561 aacgacagtt tgagaccaga gggggagatg tgcgggggca gatcgtggat gcgcaggcct
    10621 gttttaatct taatgctatt aattatggag tggtggatct gaccagtata ccttatgccg
    10681 ccagaatttt tcagcagcta ttaatcaact tgcaggtaga gcttttacag gcccggcaag
    10741 tgacggcagc cctacgtgac tggatcgatc gtgatgataa gccggtaagg ggcggtgctg
    10801 aagacgaggt gtatatgggg atggaacctc cctatctggc agccaaccag ccgatgcagg
    10861 acgtcagtga gttacgcctt atccggggaa tagatgccag gctttaccgc aaacttttgc
    10921 cgtatgtttg tgtactgcct acgtcagatc tgtctgtgaa tgtgaatact ctgctggatt
    10981 cacaggcgcc gctgctggcg gcgctctttc tgacgaaacc ggacagtctt ccggtgacgg
    11041 aattactgca gcggcgaccc cgtactggtt gggagagcgt ggcggcgttt cttgacgcgc
    11101 caccgcttaa ggacattgac accagtgcgg ccatgccggt attggccgtc agcagcaact
    11161 attttctggt tcggttacat gtgcgctccg gggaacacct gttcagtcag cagactttaa
    11221 tgcagtggag ggaggagcgt ttccggatca ttcagcgaca gtatggatta acgatgaggg
    11281 aggttccctg aaaaaaaata acaaacatgt cgaggctggt cggctttttc tgcgactgcc
    11341 ggaggatgac tcggagcctt tattgtggag gctgattgcg ccagaggaga caggagccgg
    11401 cagtgtggca gtcggagaaa cggatccgca gttagtcggt ttgctgtccc gttatcccgc
    11461 ctgggtactg gttccgtcag gcagaattgc cttccatcag gtcacgctgc cacaccgcac
    11521 gtggcgtcag cgtttgcagg tgctgccctt tcttctggaa gagcagttgg cgacagacat
    11581 tgagcagctg cacttcgcca ttcttcatca gtcgggtgac aggtgtgatg tggcggtggt
    11641 cgaaaaagcg gttatgcatc gctggttagc gtactgtgga caacttgggg ttcgggaggt
    11701 gaggttgctg cctgacgtac tgatgctgcc gttggcaact gaaggatgga gtgccgtaaa
    11761 gcttgccggg cagtggctgt ttcgccggga tcgttacgcc gggatgacgg tagagttatc
    11821 gtggctgaca catttcttgt ctcttactgc gccgccggtg attgagtcct actccgtgcc
    11881 gccagtaccg tgcccgggtc cgaagaccac tgagtggcga aatcagcccg gacgcgatct
    11941 gctgcagttg gtggccgaag gtgacgggta tgatggcgca gatctgcggc agggagaatt
    12001 tgcccgccag ggaacatggt atgcacgatt tcgcccatgg aggtatgtcg caggggctct
    12061 gctggcctgt gtttttctgg ccgggagcaa cgcggggctg gcacattatc ggctctggca
    12121 gcaggctcaa ttctggcggt atgagagcgt acgtgtctat cagcagctgt ttccgagtga
    12181 aaaggcggtg aaggatcccc gccggcagat gctgagacat ctgcaacagt cgacgggtaa
    12241 caatataccg gaactcggga gcgtgatgcg ccagcttcag caactgctgt cagaaacctc
    12301 aacgatacgc ttacaggcac tcgcctggga cagcagcagc cagacactga aaattgattt
    12361 gcaggcggct tcttttcagg cgctggaaca ctttcagcag atcgccggag aaaaatacct
    12421 catccagcct ggtgagatac gacagcagcc gcagggggta gaaagtcgtc tgatcctgag
    12481 agtgaacaat gaacgagctt aaaaaacgtt tgcagtatgt tagcccccgt gagcgcaggc
    12541 tgcttatcgg atgtgttgcc ttactggttg ttgtttttgt ctattacgtc ctctggcagc
    12601 cctggctgat acgagaggat aagtggcgga ctgtagttac ccgtgaaaaa aacacagtgg
    12661 agtggatgaa acaacaggta cccaacataa agagacggga ggcaagtatg cctgaacagg
    12721 gggagccgtt aggtttatct gcggccgtga cacgtacttc agccgcatac ggtgtatcgg
    12781 tcacccggtt gcagcctcaa ggggagcgtc tggccgtcac cttagccccg gttgaattta
    12841 atgcgctgat gcagtggctt acgcaactgg agcgtcggca ccgtattcgt accatggtat
    12901 ttgatgtggc cgcgcagggc aacccacccg gacaggtaac ggttaatcgg ctggttttgt
    12961 cccaagatga cgtaaacaag gacgcttcgc ttagtcgctg atgtcgtaat ttacagatta
    13021 atgcttgatg taaaaattgg acttataaag gaggatgtgt gagtagtttg ccggaatttg
    13081 cggttgatta tcccttgttg tggctcatct gcaccggagg gctggggggc gttaccggca
    13141 gttttcttaa cgtcgtgatt catcgtctgc cgataatgtt ggagaggcgg tggcggcgtg
    13201 aagcgctgac gctattggca cattctgtgc ctgagtccgg gccggccttt aacctgatga
    13261 taccgcagtc tcattgtccc tgctgttttc atccgctcgg catcagggat aacattcccc
    13321 tgttaagcta cctatttctg aaggggaaat cacgctgttg tggcgagcgt attcccgttt
    13381 actacccgct agtcgaaata acgaactcag tgttattcat tctggccgcg agcaggtttc
    13441 caccagggct gacattagcg gctgcctggc tttttatttc catgctgttg gttctggcgg
    13501 ttattgactg ccatacggcg ctgctgccgg atgtgctgac gttgccattg ctctggcttg
    13561 gcctgttatt cagtttacag agaggcgttg tcaccttaga ggaggcggta gtgggggcgg
    13621 tatctgggta tctctgtctg tggggactgt actggctttt tcgtttcgct accgggaggg
    13681 aagggttagg ttacggtgat tttaagctag ctgcggcatt gggagcatgg atgggttggc
    13741 aggcattacc ctcaatactt ttgtttgcct cagtcagtgg gttaatcgta acagtactgt
    13801 tgcgtattgt aacagctacg gactttaccc gcccgctgcc ttttgggccg tggctcgctt
    13861 tgagcggagt gtgccatttt ttgttgatgt agcgttcggt taccggggct agtaataaaa
    13921 acggctcagc ggataaaaga actgttgtaa accggtatca ggttaaacga tac
//
LOCUS       DBFIFS010000118        13843 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.118, whole genome shotgun sequence.
ACCESSION   DBFIFS010000118 DBFIFS010000000
VERSION     DBFIFS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.118"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            245..1354
                     /gene="livK"
                     /locus_tag="N7A07_003020"
     CDS             245..1354
                     /gene="livK"
                     /locus_tag="N7A07_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU2477643.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1402..2328
                     /gene="livH"
                     /locus_tag="N7A07_003021"
     CDS             1402..2328
                     /gene="livH"
                     /locus_tag="N7A07_003021"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU2477644.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2325..3602
                     /gene="livM"
                     /locus_tag="N7A07_003022"
     CDS             2325..3602
                     /gene="livM"
                     /locus_tag="N7A07_003022"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU2477645.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3599..4366
                     /gene="livG"
                     /locus_tag="N7A07_003023"
     CDS             3599..4366
                     /gene="livG"
                     /locus_tag="N7A07_003023"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU2477646.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4368..5081
                     /gene="livF"
                     /locus_tag="N7A07_003024"
     CDS             4368..5081
                     /gene="livF"
                     /locus_tag="N7A07_003024"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU2477647.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5200..5946
                     /locus_tag="N7A07_003025"
     CDS             5200..5946
                     /locus_tag="N7A07_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU2477648.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6306..7622
                     /gene="ugpB"
                     /locus_tag="N7A07_003026"
     CDS             6306..7622
                     /gene="ugpB"
                     /locus_tag="N7A07_003026"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU2477649.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7720..8607
                     /gene="ugpA"
                     /locus_tag="N7A07_003027"
     CDS             7720..8607
                     /gene="ugpA"
                     /locus_tag="N7A07_003027"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU2477650.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8604..9449
                     /gene="ugpE"
                     /locus_tag="N7A07_003028"
     CDS             8604..9449
                     /gene="ugpE"
                     /locus_tag="N7A07_003028"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU2477651.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9451..10521
                     /gene="ugpC"
                     /locus_tag="N7A07_003029"
     CDS             9451..10521
                     /gene="ugpC"
                     /locus_tag="N7A07_003029"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU2477652.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10518..11261
                     /gene="ugpQ"
                     /locus_tag="N7A07_003030"
     CDS             10518..11261
                     /gene="ugpQ"
                     /locus_tag="N7A07_003030"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU2477653.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALSARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11248..11688)
                     /locus_tag="N7A07_003031"
     CDS             complement(11248..11688)
                     /locus_tag="N7A07_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU2477654.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11808..13553
                     /gene="ggt"
                     /locus_tag="N7A07_003032"
     CDS             11808..13553
                     /gene="ggt"
                     /locus_tag="N7A07_003032"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU2477655.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     CASDPRSVDDLTAGY"
BASE COUNT         3092 a         3489 c         3932 g         3330 t
ORIGIN      
        1 cgccctttgt gccgttattt tatgctgaca aaggcacttt tttctgttta tctatcaata
       61 aattcagaat attatctgtt cttaatcgac tgaaaaatag ggactttaat cgctattatc
      121 acaaaatact gcgctaaacc cttaatcaga caggcaaaaa cagtgcagta taaaaaaaga
      181 acagtctgat ttgttaacac ataaaaacaa agcaacacaa catcacgaat ggggattttt
      241 gactatgaaa cggaatgcga aaactatcat cgcagggatg attgcactgg caatttcaca
      301 caccgcttta gccgacgata ttaaagttgc cgttgtcggc gcgatgtccg gcccgattgc
      361 ccagtggggc gatatggaat ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa
      421 agggggaatt aaaggcgata agctggttgg cgtagaatat gacgacgcct gcgacccgaa
      481 acaagccgtt gcggtcgcca acaaaatcgt taatgacggc attaaatacg ttattggtca
      541 tctgtgttct tcttctaccc agcctgcgtc agatatctat gaagacgaag gtattcttat
      601 gatctcgccg ggagcgacca acccggagct gacccaacgc ggttatcaac acattatgcg
      661 tactgccggg ctggactctt cccaggggcc aacggcggca aaatacattc ttgagacggt
      721 gaagccccag cgcatcgcca tcattcacga caaacaacag tatggcgaag ggctggcgcg
      781 ttcggtgcag gacgggctga aagcggctaa cgccaacgtc gtcttcttcg acggtattac
      841 cgccggggag aaagatttct ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt
      901 cgtttactac ggcggttact acccggaaat ggggcagatg ctgcgccagg cccgttccgt
      961 tggcctgaaa acccagttta tggggccgga aggtgtgggt aatgcgtcgt tgtcgaacat
     1021 tgccggtgat gccgccgaag gcatgttggt cactatgcca aaacgctatg accaggatcc
     1081 ggcaaaccag ggcatcgttg atgcgctgaa agcagacaag aaagatccgt ccgggcctta
     1141 tgtctggatc acctacgcgg cggtgcaatc tctggcaact gcccttgaac gtaccggcag
     1201 cgatgagccg ctggcgctgg tgaaagattt aaaagctaac ggtgcaaaca ccgtgattgg
     1261 gccgctgaac tgggatgaaa aaggcgatct taagggattt gattttggtg tcttccagtg
     1321 gcacgccgac ggttcatcca cggcagccaa gtgaaaatcc caccgcccgt gaaatgcggg
     1381 cgggtttaga aaggttacct tatgtctgag cagtttttgt atttcttgca gcagatgttt
     1441 aacggcgtca cgctgggcag tacttacgcg ctgattgcca tcggctacac catggtttac
     1501 ggcattatcg gcatgatcaa cttcgcccac ggcgaggttt acatgatcgg cagctacgtc
     1561 tcatttatga tcatcgccgc actgatgatg atgggcattg ataccggctg gctgctggta
     1621 gcggcgggat tcgtcggcgc aatcgtcatt gccagcgcct acggctggag tatcgaacgg
     1681 gtggcttacc gcccggtgcg taactctaag cgcctgattg cactcatctc ggcaatcggt
     1741 atgtccatct tcctgcaaaa ctacgtcagc ctgactgaag gttcgcgcga cgtggcgctg
     1801 cccagcttgt ttaatggtca gtgggtagtg ggtcatagcg aaaacttctc tgcctctatt
     1861 accaccatgc aggcggtgat ctggattgtc accttcctcg ccatgctggc gctgacgatt
     1921 ttcattcgtt attcccgtat ggggcgcgcc tgccgtgcct gcgccgaaga tctgaaaatg
     1981 gcgagcctgc ttggcattaa caccgaccgg gtgattgctt tgacctttgt gattggcgcg
     2041 gcgatggcgg cggtggcggg cgtactgctc ggtcagttct acggcgtcat taacccctat
     2101 atcggcttta tggccgggat gaaagccttt accgctgcgg tgctcggtgg gattggcagc
     2161 attccgggag cgatgattgg cgggctgatt ctggggattg cggaggcgct ctcttctgcc
     2221 tacctgagta cggaatataa agatgtggtc tcattcgccc tgctgattct ggtgctgctg
     2281 gtgatgccga ccggtattct gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata
     2341 ttgcaatggc gttgctctct gccgcgatgt tttttgtcct ggcgggcgtc tttatgggcg
     2401 tacaactgga gctggatggc accaaactgg tggtcgacac ggcttcagac atccgttggc
     2461 agtgggtgtt tatcggcacg gcggtggtct ttttcttcca gcttttgcga ccggctttcc
     2521 agaaagggtt gaaaagcgtt tccggaccga agtttattct gcccgccatt gatggctcca
     2581 cggtgaagca aaagctgttt ctcgtggcgc tgctggtgct tgcggtggcg tggccgttta
     2641 tggtctcacg cgggacggtg gatatcgcca ccctgaccat gatctacatt atcctcggac
     2701 tcgggctgaa cgtggtggta gggctttctg gtctgctggt gctggggtac ggcggctttt
     2761 acgccatcgg cgcttatacc tttgcgctgc tcaatcacta ttacggcttg ggcttctgga
     2821 cctgtctgcc gatagccggg ttgatggcgg cggcggcggg cttcctgctc ggcttcccgg
     2881 tgcttcgctt gcgcggtgac tatctggcga tcgttaccct cggtttcggc gaaattgtgc
     2941 gcatattgct actcaataac accgaaatta ccggcggtcc gaacggtatc agtcagatcc
     3001 cgaaaccgac ttttttcggc cttgagttca gccgtaccgc tcgtgaaggc ggctgggaca
     3061 cgttcagtaa tttctttggc ctgaaatacg atccctccga tcgtgtcatt ttcctctacc
     3121 tggtggcgtt gctgctggtg gtgctaagcc tgtttgtcat taaccgcctg ctgcggatgc
     3181 cgctgggtcg cgcctgggaa gcgttgcgtg aagatgaaat cgcctgccgt tcgctgggct
     3241 taagcccgcg tcgtatcaag ctgactgcct ttaccatcag cgccgcgttt gccggttttg
     3301 ccggaacgct gtttgctgcg cgtcagggct ttgtcagccc ggaatccttc acctttgccg
     3361 aatcggcgtt tgtgctggcg atagtggtgc tcggcggtat gggctcgcaa tttgcggtaa
     3421 ttctggcggc aattttgctg gtggtgtcgc gcgagttgat gcgtgatttc aacgaataca
     3481 gcatgttaat gctcggtggt ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc
     3541 tgcccatgac gcgcccgcaa ctgaagctga aaaacggcgc agcgaaagga gagcaggcat
     3601 gagtcagcca ttattatctg ttaacggcct gatgatgcgc ttcggcggcc tgctggctgt
     3661 gaataacgtc aatcttgaat tgtacccgca ggagatcgtc tcgttaatcg gtcctaacgg
     3721 tgccggaaaa accacggtct ttaactgcct gaccggattc tacaaaccca ccggcggcac
     3781 tattttactg cgcgatcagc atctggaggg tttgccgggg cagcaaatcg cccgcatggg
     3841 cgtggtgcgt accttccagc atgtgcgtct gttccgcgaa atgacggtaa ttgaaaacct
     3901 gctggtggcg cagcatcagc aactgaaaac cgggctgttc tctggcctgt tgaaaacgcc
     3961 ttctttccgt cgcgcccaaa gtgaagcgct cgaccgcgcc gccacctggc ttgagcgcat
     4021 tggtttgctg gaacacgcca accgtcaggc gagtaacctg gcctacggtg accagcgccg
     4081 tctggagatt gcccgctgca tggtgacgca gccggagatt ttaatgctcg acgaacctgc
     4141 ggcaggtctt aacccgaaag aaacgaaaga gctggatgag ctgattgccg aactgcgcaa
     4201 tcatcacaac accactatct tgttgattga acacgatatg aagctggtga tgggaatttc
     4261 ggaccgaatt tacgtggtca atcaggggac gccgctggca aacggtacgc cggagcaaat
     4321 ccgtaataac ccggacgtga tccgtgccta tttaggtgag gcataagatg gaaaaagtca
     4381 tgttgtcctt tgacaaagtc agcgcccact acggcaaaat ccaggcgctg catgaggtga
     4441 gcctgcatat caatcagggc gagattgtca cgctgattgg cgcgaacggg gcggggaaaa
     4501 ccaccttgct cggcacgtta tgcggcgatc cgcgcgccac cagcgggcga attgtgtttg
     4561 atgataaaga cattaccgac tggcagacgg cgaaaatcat gcgcgaagcg gtagcgattg
     4621 tcccggaagg gcgtcgcgtc ttctcgcgga tgacggtgga agagaacctg gcgatgggcg
     4681 gcttttttgc cgaacgtgat cagtttcagg agcgcataaa gtgggtgtat gagctgtttc
     4741 cacgtcttca tgagcgtcgt gttcagcggg caggcaccat gtccggcggt gaacagcaga
     4801 tgctggcgat tggtcgcgcg ctgatgagca atccgcgttt gttgttgctt gatgagccat
     4861 cgctcggtct tgcgccgatt atcatccagc aaattttcga caccatcgag cagctgcgcg
     4921 agcaggggat gactatcttc ctcgtcgagc agaacgccaa ccaggcgctg aagctcgctg
     4981 atcgcggcta cgtgctggaa aacggccatg ttgtgctctc cgatactggt gatgcgctgc
     5041 tggcgaacga agcagtgaga agtgcgtatt taggcgggta aattatcgat aacccggcag
     5101 ctaagttctg ccgggttatt cgtccgtata cagacttgtt tcaactgacc gggaggatta
     5161 aaaccagacc acttatttgc cacggaaagg ttccctccca tgcattatgc cgttgttgtc
     5221 atgcttgaac tgctttgctg tattcacgct taccgtaccg gacaagagcg gtactggatt
     5281 tttatcattt tctgttttcc ggtgattggc tgcgtggcgt atttcgtcat agtgatgctg
     5341 cctgaaacgg gcgctgaccg tcacgggcat actttgctga tgcgcctgca ggacaaactc
     5401 aaccctgagc gccacctgcg caaattaacc gaagaactgg cgattgccga gacgaatcaa
     5461 aaccattatg cgttggctaa tgaactggcg cgtctggggc gttatcacga agctgttccc
     5521 cattatcagc aggcgttaag cgggatattc gcccatgaag cggcaatgat gctaagcctg
     5581 gcgcaggcac agtttgctat tcaggagttc gccgcctgtc agcagacgct ggaagatgtg
     5641 atgcgttata accctgattt tcagtcggca gacgggcatt tgttatttgc cagaacgtta
     5701 gccgcacaag aaaaatacgc tgatgcggaa agcgagtttg aagtattgat ttcttattac
     5761 cccggcccgc aggcgcgtat ttactatgcg gaaatgttgg cgaaaatgag tcggttacgt
     5821 gaagccaacg agcagtatgt tgccgtggtt gatacggcta aacgctcaag acctcattat
     5881 agaaaacacc accgggaatg gattaagacc gccaatgagc ggctgaagca gagtgtggtg
     5941 caatgaaaat cttctttgcc gttagaccag tgtcgcggca atcttttgtt aaaattgaaa
     6001 aatgtagctc tcatcgggca gcaaaattgg acgcagaatt tgctgccatt tttgctcttc
     6061 atggtaagga gttctggcga ttagctggcc gttaatcgcc tcttttcagt gcattctcgg
     6121 gatatccgtg ccccatctcc cattttccct cacccgtacc gtcaccgcct tgtcatcttt
     6181 ctgacacctt actatcttac aaatgtaaca aaaaagttat ttttctgtaa ttcgagcatg
     6241 tcatgttacc ccgcgagcat aaaacgcgtg aattcgcgca ttcggtacaa caagagagat
     6301 aaacgatgaa accgttacat tatacagctt cagcactggc gctcggactg gcgttaatgg
     6361 ggaatgcaca ggcagtgacg accattccgt tctggcattc tatggaaggg gaactgggta
     6421 aagaggtgga ttctctggcc caacgtttta acgccgaaaa cccggattac aaaattgtac
     6481 cgacctataa aggcaattac gaacagaatt taagcgcggg gattgccgca tttcgtaccg
     6541 gcaatgctcc ggctattttg caggtttatg aagttggcac cgccaccatg atggcgtcga
     6601 aagccattaa accggtttat gacgtgttta aagaggcggg gattcaattc gatgagtcgc
     6661 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
     6721 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
     6781 ggttagaccc ggaacaaccg ccgaaaacct ggcaggatct ggcggactat gccgcaaaac
     6841 taaaagcctc cggcatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
     6901 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
     6961 cggatgcggt gctggagttc aacaagccgg agcaggtgaa acacatcgcc atgctcgaag
     7021 agatgaacaa gaagggcgat ttcagctacg tcgggcgtaa ggatgaatcc accgagaagt
     7081 tctataacgg tgattgcgct atgaccaccg cctcttccgg ttctctcgct aatattcgcg
     7141 agtacgccaa attcaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
     7201 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaagataaag
     7261 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
     7321 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
     7381 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
     7441 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
     7501 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
     7561 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
     7621 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
     7681 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
     7741 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
     7801 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc tgcaaagcgt cgatccgttt
     7861 ggtttctcca gccggtttgt cggcctggat aacttcgtcg cgctgtttca tgacagctac
     7921 tatatcgact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
     7981 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag tcgtttctat
     8041 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
     8101 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
     8161 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcagtatgg
     8221 aagcaaatca gctacaactt cctgttcttc tatgccgcgc tgcaatccat tccccgttcg
     8281 ttgatcgaag ccgcagccat cgacggtgca gggccgattc gccgcttctt taagattgcg
     8341 ctgccgctta tcgccccggt gagtttcttc ctgctggtag tgaacctggt gtatgccttc
     8401 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
     8461 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
     8521 gcacagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtgatgca gttccgctat
     8581 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
     8641 ataccatgct gatcctcggg atcgcggtga tcctattccc gctgtacgtg gcgtttgtcg
     8701 cggcgacgct ggataaacag gccgtctacg ccgcgccgat gacgctcatc cccggcacgc
     8761 atctgctgga aaacatccac aacatctggg taaacggggt aggcacaaat agtgcgccgt
     8821 tctggcggat gttgcttaac agcttcgtga tggcgttcag cattacgctc ggcaaaatta
     8881 ccgtctcgat gctctcggca ttcgccattg tctggtttcg ttttccgctg cgtaacctct
     8941 ttttctggat gatttttatc accctgatgt tgccggttga agtacgtatc ttcccgacgg
     9001 tggaagtcat cgccaacctg aagatgctcg acagctacgc aggtttaacg ctgccgctga
     9061 tggcctcggc gaccgcgacg tttcttttcc gccagttctt tatgacccta ccggatgaac
     9121 tggtggaagc cgcgcggatc gacggcgcgt cgccaatgcg cttcttttgc gacatcgttt
     9181 ttccgttgtc caaaactaat ctggcggcgt tgtttgtgat cacctttatt tacggctgga
     9241 accagtattt gtggccgttg ttgattatta ccgacgtgga tctcggcacc accgtggcag
     9301 ggatcaaagg gatgatcgcc actggcgaag gcaccacaga atggaactca gtgatggcgg
     9361 cgatgttgtt aaccctgatc ccaccagtag tgattgtttt agtgatgcag cgtgccttcg
     9421 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
     9481 aaaagctggg atggcaaaac ccaggtgatt aaaccgctga cccttgatgt ggcggatggc
     9541 gaatttatcg tgatggtcgg gccgtcaggc tgcgggaaat cgacgctgct gcgcatggtt
     9601 gccgggctgg agcgggtgac ggaaggcgat atctgtatca acgaccagcg ggtgaccgaa
     9661 atggagccga aagatcgcgg gattgcgatg gtgttccaga actacgcgct ttatccgcat
     9721 atgagtgttg aagaaaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
     9781 attgccgagc gcgttaaaga ggcggcgcgc attctggaac tggacggtct gcttaagcgc
     9841 cgcccgcgcg agctttccgg cggtcagcgt cagcgtgtgg cgatgggccg ggcgattgtg
     9901 cgcgatccgg cggtgttcct gtttgatgag ccactctcta acctcgatgc caagctgcgc
     9961 gtacagatgc gtcttgaact gcaacagctg caccgtcgcc tgaaaacgac ttcactctac
    10021 gttactcacg atcaggttga ggcgatgacc ctcgcccagc gagtaatggt gatgaacggc
    10081 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    10141 gtggcgagtt ttattggcag cccggcgatg aatctgctga caggccgcgt gaataacgaa
    10201 ggcacgcact tcgagctgga cggcggcatt gcgctgccgc taaacggtgg ctaccgtcag
    10261 tatgcagggc gtaaaatgac tctcggcatt cgcccggaac atatcgcgct aagctcgcag
    10321 gcagaaggcg gcgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    10381 gcgcacggac gctggggcga acagaagctg gtggtacggc tggcgcatca ggagcgcccg
    10441 acggcaggca gcacgctgtg gctgcatctg ccggaaaatc agctacatct ttttgatggt
    10501 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgcc caccgtggcg
    10561 gcggtaaact ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    10621 ataagatgat cgaatttgac gcgaagttgt cgaaagatgg cgaaatcttc ctgctccatg
    10681 acgacaatct cgaacgtacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    10741 atttactacg tgtggatgca ggcagttggt acggcaaagc gtttaaaggt gagccgctgc
    10801 cgttgctttc gcaggtggcg gaacgctgtc gcgaacatgg gatgatggcg aatatcgaaa
    10861 tcaaacccac cactggcact gggccattaa cgggcaaaat ggtggcgctg tcggcacgcg
    10921 agctgtgggc cggtatgacg ccgccgctac tgtcatcgtt tgagattgat gctttagaag
    10981 ccgcacaaca ggcagtacca gaactgccgc gcggtttgtt gctggatgag tggcgcgacg
    11041 actggcgaga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttgc
    11101 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    11161 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    11221 atgcgattga tgtgattggt ccgaacttta cggcccaata gttttcaacg gtatatcagg
    11281 ctgcggcgtg ccgggctgat tcagcatgtc gccgtttctc ctctccggca acatatgctg
    11341 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccat tggtgttggg
    11401 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    11461 attattgttt atctggtttt ctaaatgctg ttgttgcaac tgagtttgcg ttttcagttg
    11521 ctgattcagc atcccttttt gctggatttg ctgcgtttgc atctgggttt gcatccgctg
    11581 ctgactgggg atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    11641 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    11701 tctgaagata ttctttaagt ttactccttt cctgcaatca tcaggattaa ttcagagtta
    11761 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    11821 cgtttttacg ccgggtggcc attgctgctc tgttcacagg aagttgtttt agtacggtag
    11881 ccgcgccacc tgcgccgtct cctgtttctt acggcgtgga ggaagatgtc tttcatccgg
    11941 tacgcgcgaa acaggggatg gtggcatctg tagatgctac tgccactcag gtgggggtgg
    12001 atatcctcaa ggagggcggg aatgccgttg atgccgccgt ggctgtgggc tacgcgctgg
    12061 cggtaacgca tccgcaggca gggaatctgg gcggtggtgg ttttatgctg atccgctcga
    12121 aaaatggcaa taccaccgct atcgatttcc gcgaaatggc acccgccaaa gcgacccgcg
    12181 atatgttcct tgatgaccag ggcaacccgg acagcaaaaa atcactcact tcgcatctgg
    12241 cttccggcac gcccggtacg gtcgctggtt tctcgctggc gttggataaa tacggcacaa
    12301 tgccgttgaa caaagtggtg cagccagcgt ttaaactggc acgcgatggt tttatcgtta
    12361 acgacgagct ggctgacgat ctcaaaacct acgggagcga agtgctgccg aatcacgaaa
    12421 acagcaaggc tatcttctgg aaagagggcg agccgctgaa aaagggcgac aagctggtgc
    12481 aggcgaacct ggcaaagagc ctggagatga ttgccgaaaa cggcccggac gaattctaca
    12541 aaggcacgat agcggaacag attgcccagg agatgcagaa aaacggtggc ctgatcacta
    12601 aagaagattt agcggcgtat aaagcggtcg aacgcacacc gataagcggc gattatcgcg
    12661 ggtatcaggt ttactccatg ccaccgccat cctccggcgg gatccatatc gtacaaatcc
    12721 tcaatattct ggaaaacttc gatatgaaga aatacggttt tggcagcgcc gacgcgatgc
    12781 agatcatggc agaagcggag aaatatgcct acgctgaccg ctcggaatat cttggcgacc
    12841 cggattttgt gaaagtcccg tggcaggcgc tgaccaataa agcctatgcc aaatctattg
    12901 ccgagcaaat cgatatcaat aaagcgaaac cgtccagcga gattcgcccc ggcaagcttg
    12961 cgccttatga gagtaatcaa actacccatt attcggtggt ggataaagac ggtaacgcgg
    13021 tggcggtgac ctatacgctg aacaccacct tcggtacggg tattgtcgcg ggcgagagcg
    13081 gtattttgct caataaccaa atggatgatt tctctgccaa accgggcgta ccgaacgttt
    13141 acgggctggt gggcggtgat gccaacgccg tcgggccgaa caaacgcccg ctgtcatcga
    13201 tgtcaccaac cattgtggtg aaagacggta aaacctggct ggtcaccggt agccctggcg
    13261 ggagcctgat catcactaca gtgcttcaaa tggtggtgaa tagcatcgat tatggcatga
    13321 acgtcgccga agcgaccaat gcgccgcgtt tccaccatca gtggttgccg gacgagctgc
    13381 gtgtcgaaaa agggtttagc ccggatacgc tcaagctgct ggaagcaaaa ggtcagaaag
    13441 tggcgctgaa agaggcgatg ggcagtacac aaagcattat ggttgggccg gacggtgagt
    13501 tgtactgcgc atccgacccg cgctcggtgg atgatttaac ggcggggtac taaggttagc
    13561 ggccctcttc gtgggaagag ggctgttttc ttgaacaaga taaattagtg tctggattgc
    13621 ttttactatt gctccgtaat atataatcat aattatcctg aatgctttta aggggcttag
    13681 gttatgcttt cttttcaaga gtatgcagaa tagtctttca tttaccgtga aaataggacg
    13741 tgcgataatt acttcctgat attcaggaaa ggaattaact atgctattaa ataaactgta
    13801 gttatagttc attttagagt gtaatttatt gatttaaata tca
//
LOCUS       DBFIFS010000119        13708 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.119, whole genome shotgun sequence.
ACCESSION   DBFIFS010000119 DBFIFS010000000
VERSION     DBFIFS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.119"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(69..233)
                     /gene="yojO"
                     /locus_tag="N7A07_003033"
     CDS             complement(69..233)
                     /gene="yojO"
                     /locus_tag="N7A07_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU2477656.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            342..836
                     /gene="napF"
                     /locus_tag="N7A07_003034"
     CDS             342..836
                     /gene="napF"
                     /locus_tag="N7A07_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU2477657.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            826..1089
                     /gene="napD"
                     /locus_tag="N7A07_003035"
     CDS             826..1089
                     /gene="napD"
                     /locus_tag="N7A07_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU2477658.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1086..3572
                     /gene="napA"
                     /locus_tag="N7A07_003036"
     CDS             1086..3572
                     /gene="napA"
                     /locus_tag="N7A07_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU2477659.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3579..4274
                     /gene="napG"
                     /locus_tag="N7A07_003037"
     CDS             3579..4274
                     /gene="napG"
                     /locus_tag="N7A07_003037"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU2477660.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4261..5124
                     /gene="napH"
                     /locus_tag="N7A07_003038"
     CDS             4261..5124
                     /gene="napH"
                     /locus_tag="N7A07_003038"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU2477661.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5121..5570
                     /gene="napB"
                     /locus_tag="N7A07_003039"
     CDS             5121..5570
                     /gene="napB"
                     /locus_tag="N7A07_003039"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU2477662.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5580..6182
                     /gene="napC"
                     /locus_tag="N7A07_003040"
     CDS             5580..6182
                     /gene="napC"
                     /locus_tag="N7A07_003040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU2477663.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6195..6818
                     /gene="ccmA"
                     /locus_tag="N7A07_003041"
     CDS             6195..6818
                     /gene="ccmA"
                     /locus_tag="N7A07_003041"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU2477664.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            6815..7477
                     /gene="ccmB"
                     /locus_tag="N7A07_003042"
     CDS             6815..7477
                     /gene="ccmB"
                     /locus_tag="N7A07_003042"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU2477665.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7519..8256
                     /gene="ccmC"
                     /locus_tag="N7A07_003043"
     CDS             7519..8256
                     /gene="ccmC"
                     /locus_tag="N7A07_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU2477666.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8253..8462
                     /gene="ccmD"
                     /locus_tag="N7A07_003044"
     CDS             8253..8462
                     /gene="ccmD"
                     /locus_tag="N7A07_003044"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU2477667.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8459..8938
                     /gene="ccmE"
                     /locus_tag="N7A07_003045"
     CDS             8459..8938
                     /gene="ccmE"
                     /locus_tag="N7A07_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU2477668.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8935..10878
                     /gene="ccmF"
                     /locus_tag="N7A07_003046"
     CDS             8935..10878
                     /gene="ccmF"
                     /locus_tag="N7A07_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU2477669.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10875..11432
                     /gene="dsbE"
                     /locus_tag="N7A07_003047"
     CDS             10875..11432
                     /gene="dsbE"
                     /locus_tag="N7A07_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU2477670.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11429..12481
                     /gene="ccmH"
                     /locus_tag="N7A07_003048"
     CDS             11429..12481
                     /gene="ccmH"
                     /locus_tag="N7A07_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU2477671.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12516..13163)
                     /gene="narP"
                     /locus_tag="N7A07_003049"
     CDS             complement(12516..13163)
                     /gene="narP"
                     /locus_tag="N7A07_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU2477672.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13483..>13708
                     /locus_tag="N7A07_003050"
     CDS             13483..>13708
                     /locus_tag="N7A07_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477673.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSN"
BASE COUNT         2927 a         3443 c         4012 g         3326 t
ORIGIN      
        1 cagatttatt aatttgcttt atgaataact taaagaatat tcagacaatt ttacctgctc
       61 ttttagtgtt aaattctgat gagagagcaa accagcagaa atatctctgg tgaattaatc
      121 gctatataaa tatatttata accatttgaa atgtgagcaa aagcccgttt ttgccacgct
      181 tcccgccact cttttgatcc tgctacaggt tttacctcga tcggggtatg catctttgac
      241 acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg tttatttggt
      301 cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc
      361 gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt ccgccctggt
      421 ccggtgatga atctcatttt ctgacccatt gcacccgctg cgacgcctgt attaatgctt
      481 gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata
      541 acgagtgcag cttctgttac gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc
      601 gccacaccag ggcctgggat ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt
      661 cagttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc tttcgcccga
      721 cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga tgtggagcat
      781 gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca cactaactgg
      841 caagtttgca gcctggttgt gcaggccaaa agcgaacgaa tttcagacat cagcacccaa
      901 ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt
      961 gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac
     1021 gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca aggtgaggaa
     1081 acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg
     1141 cggctgccgg tctcagtgtg ccgggcgttg cccgcgccgt tgttggtcag caggaagcca
     1201 ttaaatggga taaagcgccg tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa
     1261 cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc
     1321 tgaactgcat taagggctat ttcctgccca agatcatgta cggtaaagac cgtttgacgc
     1381 agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt accccaatca
     1441 cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg aaagaaaaag
     1501 ggccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa ggttatgccg
     1561 cgtccaagct gtttaaagcg ggcttccgtt cgaacaacat cgacccgaat gcgcgtcact
     1621 gtatggcgtc ggcagtagtt ggctttatgc gaacctttgg tatggatgag ccgatgggct
     1681 gctatgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgcaaac atggcggaga
     1741 tgcacccgat cctctggtca cgcatcacta accgtcgtct ctctaaccag gacgtcaccg
     1801 tggcggtgct ttcaacctac cagcatcgta gcttcgagct ggcggataac ggcatcatct
     1861 ttacgccgca atctgacctg gtgatcctga actacatcgc caactatatc attcaaaaca
     1921 atgcgataaa tcaggacttc ttcagcaagc acgttaacct gcgcaaaggg gcgacggaca
     1981 tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat ccgggttctg
     2041 acgcctccga accgatgagc tttgaagatt acaaagcctt cgttgctgag tatacgctgg
     2101 aaaaaaccgc tgaaatgact ggcgtaccaa aagatcagct ggaacaactg gcgcagctgt
     2161 atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac cagcatactc
     2221 gtggcgtgtg ggccaacaac ctggtctaca acctgcacct gctgaccggc aaaatttccc
     2281 agccgggttg cggtccgttc tccctgaccg ggcagccttc cgcgtgtggt actgcgcgtg
     2341 aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtaactaac gagaaacatc
     2401 gtgatatctg cgagaagaag tggaatatcc cgagcggcac cattccggcg aaaatcggtc
     2461 tgcatgcggt agcacaagac cgtgcgctga aagacggcaa gctgaatgtt tactggacca
     2521 tgtgtaccaa caacatgcag gccgggccga acattaatga agagcgtatg ccgggctggc
     2581 gcgatccgcg caacttcatc atcgtctccg atccgtatcc gacagtcagt gcgctggccg
     2641 ccgacttgat cctgccgacc gcaatgtggg tagaaaaaga gggggcttac ggtaacgccg
     2701 aacgccgtac tcagttctgg cgtcagcagg tacaggcgcc aggcgaagcg aaatcggacc
     2761 tctggcagtt agtccagttc tcccgccgct tcaaaactga agaagtatgg ccggaagagc
     2821 tgctggcgaa gaaaccggaa ctgcgtggca aaacgctgta cgaagttctg tatgccaccc
     2881 ccgaagtgag caaattcccg gtatccgaac tggcggaaga tcagctgaac gatgaatccc
     2941 gcgagctggg cttctatctg caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg
     3001 gtcacggtca cgatctcgcg ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc
     3061 cggtggtgaa cggtaaagaa acgcagtggc gttacagcga aggtaacgac ccgtacgtga
     3121 aagcgggcga aggctataag ttctacggta aaccggatgg taaagcggtg atcttcgcgc
     3181 tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg ctctctaccg
     3241 gacgcgtcct ggagcactgg cacaccggca gtatgactcg ccgtgtgccg gaactgcacc
     3301 gtgccttccc ggaagcggtc ctgtttattc acccgctgga tgcgaaagcg cgcgatctgc
     3361 gccgtggcga caaagtgaaa gtggtttctc gccgtggcga agtgatctcg attgtcgaaa
     3421 cgcgcggtcg taaccgtccg ccgcagggcc tggtgtacat gccgttcttc gacgccgcac
     3481 agctggttaa caaactaacg ctggacgcga ccgatccgct ctcgaaagag acggacttca
     3541 agaagtgcgc ggtcaaactg gagaaggtgt aagccattat gtcccggtca gcgaaacctc
     3601 aaaatggtcg ccgccgcttt ctgcgcgatg tcgttcgcac agcaggcggg ctggctgccg
     3661 tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc
     3721 cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg
     3781 ttcaggcttg cccttacgac accttaaaac tggcgacgct ggcctctggc ctgtcggcgg
     3841 gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac attccgtgcg
     3901 ccaaagtgtg cccgagcggt gcgctggatc gtgagattga atcgatcgac gacgcgcgga
     3961 tggggctggt ggtactggtg gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg
     4021 atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag ctggagcgca
     4081 acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac gcctgtaccg
     4141 gttgcggtaa gtgcgaaaaa gtatgcgtgc tggaacaacc ggcaatcaag gtgttaccgc
     4201 tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggctggctg gaggggaaca
     4261 atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg agaaaaaagg ctggtggcgc
     4321 agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg
     4381 agcggtccgt ggtttggtgt gtggatcttg cacggcaact atagcagtag cctgttattc
     4441 gacaccgttc cgctgaccga tccgctgatg acgctgcaaa gtcttgccag tggtcatctg
     4501 cctgccacgg tggcgttgac cggtgcggtg attatcaccg tgctctacgc cctggcagga
     4561 aagcgattat tttgcagctg ggtctgcccg ctgaacccga ttaccgactt agcgaactgg
     4621 ttacgcaggc gctttgacct caatcagtct gcgacgatcc ctcgccacat tcgctacgtg
     4681 ctgttggtgg tcattctggt gggatcagcc ttaactggca cgctcatctg ggaatggatc
     4741 aaccccgttt ccctgatggg gcgtagcctg gtaatgggct tcggcagcgg cgcgctgctt
     4801 attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg cgggcacatt
     4861 tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc
     4921 agcgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc ggaaccgcat
     4981 gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc
     5041 atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat aactacacga
     5101 tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga
     5161 cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc gtcgatttta
     5221 gccagtcgcc ggaagtatcc gggacgcagg aaggggccat tcggatgcca aaagagcagg
     5281 atcggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt
     5341 atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa agctatcgca
     5401 ccactggcgc gccgcgtatc agtcctactc actttatgga cagcgacggc aaagtgggcg
     5461 cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag gccgataccg
     5521 cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa gaggttatta
     5581 tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc
     5641 ccagccgtct ggcgctgggg acgctgctgt tgatcggttt tgtcggcggc atcgtcttct
     5701 ggggcggctt taacactggg atggaaaaag ccaataccga agagttctgc attagctgcc
     5761 acgaaatgcg taacacggtg tatcaggaat acatggattc cgtgcactac aacaaccgta
     5821 gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg ccgaagatga
     5881 tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt attgacacgc
     5941 cgcagaaatt tgaagctcat cgtctgacga tggcacagaa tgagtggcgg cgcatgaagg
     6001 acaataactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat acaaccgccc
     6061 agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa acctgtattg
     6121 attgccataa agggatagcg cacaagctgc ccgatatgcg tgaagtcgag ccaggttttt
     6181 aacagggtta ttgcgtgggt atgcttgaag tcagagagtt actttgtgag cgggatgaac
     6241 gaaccttatt tagtggcttg tcatttacgc tgaacgcagg agagtgggta caaatcaccg
     6301 gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg tctcgccctg
     6361 acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac agctaccatc
     6421 aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg gcgttagaaa
     6481 atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc ctggcgcagg
     6541 ccgggcttgc cggattcgaa gatattccgg taaatcaact ttctgccggg caacaacgcc
     6601 gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatcctc gacgagcctt
     6661 ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg cagcatacag
     6721 agcagggggg gattgtgatt ctcactaccc accagccgct caacgttgct gaaagtaaaa
     6781 ttcgccgcat ttcactgacg caaacgaggg ccgtatgatg ttctggcgca ttttccgtct
     6841 tgagctgcgc gtagcgtttc gccatagcgc tgaaatcgcc aacccgctgt ggttcttcct
     6901 gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc tggcacgtat
     6961 tgcaccgggc attatctggg ttgccgcgct gctttcatcc ttgctggcgc tggaacgact
     7021 gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc cgttaccctt
     7081 gcccgccgtt gtgctggcga aggtgatggc gcactggatg gtaaccggtc tgccgttact
     7141 catcctttcg ccactggtag caatgctact gggaatggat gtttatggct ggcaagtgat
     7201 ggcgttaacg ctactgctgg gaacgcctac gcttggcttt ctcggtgcac cgggcgtggc
     7261 gctgacagtg ggacttaagc gcggtggtgt gctgctcagc atactggtgt taccgctgac
     7321 tatcccatta ctcatctttg ccaccgccgc gatggacgcg gcttctatgc atttgcccgt
     7381 tgacgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat taagtccttt
     7441 tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg atttttgttc
     7501 gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc accacggctg
     7561 tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtagt cgtgcttacc
     7621 gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg aaatagctac
     7681 cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta tgcatcaatg
     7741 gcggtggcgg cgtttattgg ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg
     7801 gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg ctctgcatgg
     7861 ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc tgaactggtg
     7921 ctgctgtttt tgtatgtggg tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg
     7981 gcgggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc gattattcat
     8041 tactccgtgg agtggtggaa caccctgcat cagggatcaa cgcggatgca gcaaagtatc
     8101 gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct cctctctgcc
     8161 acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg
     8221 gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct tcctggaatg
     8281 aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg atgaccgtta
     8341 ttccgctggt ggttttggtc gtgcactcgg tgatgcagca tcgcgcaatt ctgcgtggtg
     8401 tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat
     8461 gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgcc gtgttggcag ggctggcgct
     8521 gactatcggt ctggtgctat atgcgctgcg ctcgaatatc gatctctttt atacgccggg
     8581 ggaaattctc tacggcaagc gtgaaactca gcagatgccg gaagtcggtc agcgtctgcg
     8641 cgttggcggg atggtgatgc cgggtagtgt gcagcgcgat cccaattcgc tgaaagtgac
     8701 cttcaccatt tacgacgctg aaggctcagt ggatgtctct tacgaaggca ttttgccgga
     8761 tctgttccgt gaagggcagg gcgttgtggt gcagggcgag ctggaaaaag gcaatcatat
     8821 cctcgcaaaa gaagtgctgg cgaaacatga tgaaaactac acgccgccag aagttgagaa
     8881 agcgatggaa gctaaccacc gtcgcccggc gagtgtttat aaggacccag catcatgatg
     8941 ccggaaattg gtaacgggct gctgtgcctg gcgctgggaa ttgcgctgct gctgtccgtg
     9001 tatccgctat ggggcgtggc gcgcggagat gcgcgcatga tggcgtcttc ccgcttgttt
     9061 gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tgctggtcaa tgctttcgtg
     9121 gtcaacgact ttaccgtcac ctatgttgcc agcaactcca atacccagct tccggtgtgg
     9181 tatcgcgtgg cggctacctg gggcgcacat gaaggctcac tgctgctgtg ggtgctgctg
     9241 atgagcggct ggacctttgc ggtggcgatt tttagtcagc gtattccgct ggatattgtg
     9301 gcccgcgtac tggcgataat ggggatggtc agtgtcggct ttttgctgtt cattctcttt
     9361 acctctaacc cattctcacg cacgctgccg aacttcccga ttgaagggcg cgatcttaac
     9421 ccgctgttac aggatccggg gctgatcttc catccgcctc tgctttatat ggggtacgtg
     9481 ggtttctcgg tggcgtttgc ttttgccatt gcttctttgc tgagcgggcg tctggacagc
     9541 acttatgcgc gttttactcg tccgtggacg ctggcagcgt ggatcttcct gacgctcggc
     9601 atcgtgctcg gttccgcatg ggcctattac gaactcggct ggggtggctg gtggttctgg
     9661 gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct gatgcactca
     9721 ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct ggcaatcagt
     9781 gccttctcgt tgtgtctgtt ggggactttc ctcgtgcgtt ccggcgtgct ggtatcggta
     9841 cacgcgtttg cgtctgatcc ggcgcgcggt atgtttatcc tcgcctttat ggtgctggtg
     9901 attggcggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac
     9961 aatgcgctgt ggtcgcggga atctttgctg ttagcgaaca acgttttgct ggtcgccgcg
    10021 atgctggtgg tgttgctggg gacgctgctg ccgctggtgc acaagcaact gggactgggc
    10081 agtatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat ggtgccgttt
    10141 gcgctactgc tgggtgtcgg tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc
    10201 cgcaatttat tgattatcgc cttcatctct acgctggtgc tgtctctgct gttgccgtgg
    10261 ctgttcgaaa gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt
    10321 gcggtgctgg caattgcgga agctgcgcta cgtatttcac gcggcacgaa aaccaccttc
    10381 agttattggg ggatggtggc ggctcacctg gggctggcag tgacaattgt tggcattgcc
    10441 tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga tagcgtcgat
    10501 attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc gaactggcgt
    10561 ggcggtgtgg cgactatcgg cgtaacgcga gagggtaaac cggaaacggt gctgtatgcg
    10621 gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcaat tgacggcggc
    10681 atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc gtgggctgtg
    10741 cgtctttact acaaaccatt tgttcgctgg atttgggcgg gcgggctgat gatggcgttg
    10801 ggcggactgc tgtgtctgtt tgatcctcgc tatcgtaagc gcgtgagccc gcaaaaaact
    10861 gcgccggagg ctgtatgaag cgcaaagtat tgttaattcc gttgattatc ttcctggcga
    10921 ttgccgcggc gctgctgtgg cagctggcgc gtaatgccga aggggatgat ccgaccaatc
    10981 tggaatcggc gctcattggc aagcctgtgc cgaagtttcg tctcgaatca ctggacaatc
    11041 cggggcagtt ttatcaggcg gatgtgctga ctcagggcaa accagtgctg cttaacgtct
    11101 gggcgacctg gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag ctttctgcgc
    11161 agggcatccg cgtggtcggc atgaactata aagacgatcg ccagaaggca atcagctggc
    11221 tgaaagagct gggcaatcct tacgcgctaa gcctttttga tggcgatggc atgttagggc
    11281 tggatctcgg tgtctatggc gcgccagaaa cgtttcttat tgacggcaac ggcatcattc
    11341 gctatcgcca tgcgggcgat ctcaatcctc gcgtctggga agaagagatc aagccgctgt
    11401 gggagaaata cagtaaggag gccgcacaat gaggttttta ttgggcgtgc tgatgctgat
    11461 gatctccggc tcagcgctgg cgaccatcga cgtgttgcag tttaaagatg aagcacagga
    11521 acaacagttc cgtcagctca ctgaagaact gcgctgcccg aaatgccaga ataacagcat
    11581 tgccgattcc aactcgatga ttgccaccga cctgcgccag aaagtgtatg aactgatgca
    11641 ggaaggcaaa agtaagaaag agattgtcga ttatatggtg gcgcgttacg gcaacttcgt
    11701 cacttacgat ccgccgttaa cgccgctgac cgtgctgctg tgggtgcttc cggtagtggc
    11761 tattggtatt ggcggttggg tcatatacgc ccgttcgcgg cgtcgggtac gcgtggtacc
    11821 ggaagcgttt cctgaacaaa gcgtgccgga aggtaagcgt gccggatatg ttgtttatct
    11881 gccgggtatt gtggtggcgt taattgtggc tggcgtcagc tactaccaga ctggcaatta
    11941 tcagcaggtg aaaatctggc agcaggccac ggcacaggct ccggcgttgc tggacagggc
    12001 gctggatccg aaagccgatc cgctcaacga agaagagatg tcgcgccttg cgctggggat
    12061 gcgtactcaa ctgcaaaaaa atccgggaga tatagaaggt tggattatgt tgggtcgcgt
    12121 tggcatggcg ctgggtaacg ccagtatcgc caccgatgca tacgctactg cgtatcgcct
    12181 cgatccgaaa aacagcgatg ccacactggg ttatgctgaa gcgttgacac gttcatctga
    12241 tcccaacgac aaccgcctcg gcggcgaact gctgcgccag ttggtgagaa cggaccatag
    12301 caatattcgt gtgttaagca tgtatgcgtt taatgccttt gagcagcagc gatttggcga
    12361 agccgttgcc gcgtgggaga tgatgttgaa actcttacct gccaacgata ctcgccgtgc
    12421 ggtgattgaa cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg agagtaaata
    12481 aaaagaggcc cgatggtgat gccatcgggc tatttttatt gtgccccgcg ttgttgcagg
    12541 aacagaatgg tggctgccac gcgtgagcgg acattgagtt tgcgcagtag attgcgaata
    12601 tgtactttta ctgtctgctc ggaaatattc aacaccgagg caatctgttt atttgacagc
    12661 ccctgtgcca gctcgtgcag aacatccagc tcgcgctccg tcagcacgct gaagggatct
    12721 tcttccgcgc caaacatttc acgttcatgt aagtactgat tgacgcgttc gctaaagact
    12781 ttgctgcctt tcgctccggc acgaatcgct tccagcaaca cttccgggtc gctgtctttc
    12841 aacagataac cgtctgcgcc tgcgtctatc agtgcaaaga catcgctgga ggcatctgat
    12901 acggtcagga taataatttg cgcggtaacg ccatccctgc gcaaggcatt gagggtatcc
    12961 aggccactca tacctttcat attgagatcc agcaagatca cgtcgatatc cagtctattc
    13021 gccagatcga tagcactcgc gccgtcgccc gcttcggcga ccacttcaaa gccaggatca
    13081 agctccagta actgacgaac accgcgtcgc ataagtggat gatcatccac aatcatcacc
    13141 tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt ttattagaat cattatgttt
    13201 gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta ccccatttta tgtatggaca
    13261 tgtagcgaaa actacaacct tgtcgttcgc tttggcatca ttttcattat aaagacggaa
    13321 ttttgcatca gcttctcatc aagaaaaaca actaaaaaat caatcacaat acttgtgcat
    13381 ttctttaaac agtttttaaa aactgactcc gggtatggag ctatgggtat tttctgtacc
    13441 caatgctttt aactgcaatt aatttcatag gatgaaagct caatgcatca atctggttct
    13501 gtttctcttt gtcgttccgc aatatctgtt ctggtagcta cagcgttata ttcacccata
    13561 gcattggcat caacagttga gtatggtgag acagttgatg gtgttgtcct ggaaaaagat
    13621 atccagctgg tttatgggac cgccaataat acgaaaatca atcctggcgg agaacagcat
    13681 attaaagaat ttggtataag tagtaata
//
LOCUS       DBFIFS010000120        13490 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.120, whole genome shotgun sequence.
ACCESSION   DBFIFS010000120 DBFIFS010000000
VERSION     DBFIFS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.120"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            81..1694
                     /gene="mppA"
                     /locus_tag="N7A07_003051"
     CDS             81..1694
                     /gene="mppA"
                     /locus_tag="N7A07_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU2477674.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVCSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1745..2776)
                     /gene="ynaI"
                     /locus_tag="N7A07_003052"
     CDS             complement(1745..2776)
                     /gene="ynaI"
                     /locus_tag="N7A07_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU2477675.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTISLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3020..3277
                     /gene="ynaJ"
                     /locus_tag="N7A07_003053"
     CDS             3020..3277
                     /gene="ynaJ"
                     /locus_tag="N7A07_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU2477676.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3327..4277)
                     /gene="uspE"
                     /locus_tag="N7A07_003054"
     CDS             complement(3327..4277)
                     /gene="uspE"
                     /locus_tag="N7A07_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU2477677.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4429..5181)
                     /gene="fnr"
                     /locus_tag="N7A07_003055"
     CDS             complement(4429..5181)
                     /gene="fnr"
                     /locus_tag="N7A07_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU2477678.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDTLAQLAGHTRNVA"
     gene            complement(5376..5891)
                     /gene="ogt"
                     /locus_tag="N7A07_003056"
     CDS             complement(5376..5891)
                     /gene="ogt"
                     /locus_tag="N7A07_003056"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU2477679.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(5902..7428)
                     /gene="abgT"
                     /locus_tag="N7A07_003057"
     CDS             complement(5902..7428)
                     /gene="abgT"
                     /locus_tag="N7A07_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU2477680.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(7465..8910)
                     /gene="abgB"
                     /locus_tag="N7A07_003058"
     CDS             complement(7465..8910)
                     /gene="abgB"
                     /locus_tag="N7A07_003058"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU2477681.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(8910..10220)
                     /gene="abgA"
                     /locus_tag="N7A07_003059"
     CDS             complement(8910..10220)
                     /gene="abgA"
                     /locus_tag="N7A07_003059"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU2477682.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10396..11304
                     /gene="abgR"
                     /locus_tag="N7A07_003060"
     CDS             10396..11304
                     /gene="abgR"
                     /locus_tag="N7A07_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2477683.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            11634..12197
                     /gene="smrA"
                     /locus_tag="N7A07_003061"
     CDS             11634..12197
                     /gene="smrA"
                     /locus_tag="N7A07_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU2477684.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12218..13450)
                     /gene="dgcM"
                     /locus_tag="N7A07_003062"
     CDS             complement(12218..13450)
                     /gene="dgcM"
                     /locus_tag="N7A07_003062"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU2477685.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         3532 a         3275 c         3325 g         3358 t
ORIGIN      
        1 tctgatggaa gttgtttgca tttctttaag gcgaaacaaa taattacgca tcaattttaa
       61 tgtcggttag agggaaactt atgaagcact ctgtttcagt cacgtgttgt gcgctgttgg
      121 tcagcagcat ttctctttcg tatgctgcag aagttccgag cggcacagta ctggcagaga
      181 agcaggagct ggtgcgccac attaaagatg agcctgcgtc gctggatccc gctaaagccg
      241 tgggcctgcc agagattcag gtcattcgcg atctgtttga aggactggtg aatcagaacg
      301 agaaagggga gattgtcccc ggcgttgcga ctcagtggaa aagtaatgac aaccgtatct
      361 ggacttttac cctgcgcgat aacgcaaaat gggcggatgg cacaccggta acggcgcaag
      421 attttgtctg cagctggcag cgtctggtgg acccgaaaac gctatcgcca tttgcatggt
      481 ttgccgcgct ggcgggaatc aacaacgcac aggcgattat tgatggtaaa gctacacctg
      541 accagcttgg cgtcaccgca gttgatgtcc atactttgaa aattcagctt gataaaccgt
      601 tgccgtggtt tgtgaattta accgctaact ttgccttctt cccggtgcaa aaagccaacg
      661 tagaaagcgg taaagagtgg acgaaacccg gaaatctgat cggcaatggc gcttatgttc
      721 ttaaagagcg cgtagtcaat gaaaaactgg tcgtggtacc gaatacccat tattgggata
      781 acgccaaaac ggtactgcaa aaagtgacct tcctgccaat taatcaggaa tccgcagcca
      841 ctaagcgtta cctcgcgggg gatattgata tcaccgaatc cttcccgaaa aatatgtatc
      901 agaagctgtt gaaggatatt ccggggcagg tttatacgcc gccgcagctc gggacctatt
      961 attatgcgtt taacacgcaa aaagggccga cggcagatca gcgcgttcgt ctggcattaa
     1021 gtatgacgat agatcgccgc ctgatgaccg aaaaagtatt agggacgggc gaaaagccag
     1081 cgtggcattt tacaccagat gttaccgccg gatttacgcc ggaaccttcg ccgtttgaac
     1141 aaatgagtca ggaagaactg aatgcgcagg caaaaacttt gttgagcgca gctggttatg
     1201 gtccgcaaaa accgctgaag ctgacgcttt tgtataacac ttcagaaaac catcaaaaaa
     1261 ttgcgattgc tgtagcatcg atgtggaaaa agaaccttgg cgtagatgtt aaattgcaaa
     1321 atcaggaatg gaaaacctat atcgatagcc gtaacaccgg caattttgat gttatccgcg
     1381 cctcatgggt gggggattat aatgaaccct ccactttcct gacattatta acgtcaacgc
     1441 attcaggaaa tatttcacgc tttaacaatc cggcatacga caaagttctg gcccaggcat
     1501 cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa cgcggcagaa aaaatcctca
     1561 tggagcaagc accgattgca ccaatttatc aatataccaa tggacgatta atcaaaccgt
     1621 ggctgaaagg ttatcccatt aataaccctg aggatgtggc gtacagtcgg actatgtata
     1681 ttgtgaagca ttgatgtgat gggaactggc gttacccttg tggataacgc cagtgatgtc
     1741 tgatttagcg accctgttcc ggcggtgtaa tgttatccat atacagcgtc tggctgggga
     1801 aggcaaagtc cgcgccgtgt gactgtacaa tatcgataat cttcaaataa acgtcttgct
     1861 gtgcagcaag ccactcagcc cataccgtgg ttttggtaaa gcaataaacc ataatattca
     1921 atgacgagtc agcaaactgg ttgaaataaa ccagtaaggt ttgtcgctgg tcgatggccg
     1981 ggtgattttt cagcatctca cgtacggctt cgacgataac gcccactttt gccgcatcct
     2041 cataacgtaa actaatggtc gtggtaatgc ggcggttggt cattcgtcct gggttttcta
     2101 cgctgatcga tgaaaacagc gagttcggta cgtacaatgg acgattatca aaggtcgtaa
     2161 ttttggtaat tcgccagcca atttccgcta ctgtaccttc gatatttctg tccggtgaac
     2221 ggatccagtc gccaatactg aaaggacggt cgaaatagag catgatcccg gaaaagaaat
     2281 tactcagaat atctttgcca gccataccga cagccagacc accaatacca ccaaaggtca
     2341 gcaagccaga aaggctcatg ccgaaatgtt cgccataaag cagaacaagc accacaataa
     2401 tggtgatttt gataatgcgc gacataatcc gcgcactggt gatatcgcga ccctttttaa
     2461 tctgctgttt ttcaaactga ttaatcagca aaaatagctt aatcgtcaga ataaccgcaa
     2521 tcagggacgt acagataaaa tcgataacgc ctggagtgat aaatttgagt ttatagtttt
     2581 ctataacata attaataatg ctaccaacag cactgataat tatggtgtag attaaaaatt
     2641 gcaccgcatg gaataaaaag ccttttcttt tacgatttcc gcggcgaaac caaaagctca
     2701 tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag attaagcgca ttatttgtaa
     2761 acagttcagc gatcattgtt ttatcaggct cctccagata attgtcgtca tgccggaaac
     2821 ccctggcggg gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa
     2881 gtagcaacat tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt
     2941 ttcagagcag acaatggtga aatgtcatgg tattgttacg tttaggtaac aagaaatttg
     3001 tctgcacaag gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt
     3061 tctctttggt ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgactat
     3121 cggcggcgtt atagaacagt acaatattcc gctgtcggag tggacgacat caatgtatgt
     3181 gattcagtca tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc
     3241 gttgggaatt tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc
     3301 gggccttgtc agttattgag gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt
     3361 gtctgatact ggtcaggttt aatgaccaac agatcgcagc gaagatgatc aatcacctgt
     3421 tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc gtcctaccgt gcccagaacc
     3481 acaatccccg cctgtaagtg ctccgccaaa tcaggaatca cctcttctgg cagacctttt
     3541 tctacgtgcg tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc
     3601 aaatgttgcc cacgaatggc atcgttataa acgctcggat caaattccgg cagttcaatc
     3661 gcgatattaa ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact
     3721 tgttctgcca gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc
     3781 tcttcactgg cgagattcac cgccaccagc gccttacctc cttccggcca cggctggtct
     3841 ttcaccatcc acaccgggct tgggcatttg cgtaacaggt gccagtccgt tggcgtaaaa
     3901 atcaccgctt ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgcccgccg
     3961 ctgatcactt cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca
     4021 atgggaacgc cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg
     4081 ctgatgacgc cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg
     4141 tatgagaagt catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa
     4201 taaacagctc gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg
     4261 ttctgataca tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa
     4321 aagattaacc catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc
     4381 agaaaaattt aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt
     4441 acgcgtatga ccagcaagct gggccagcgt atcgttattt tcgatggtga tgtatttacc
     4501 tttgactgcc agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac
     4561 cgtcaggccc agatagttac cgatatcgcc acgagtcatc gtcaggcgga attcacgagg
     4621 ggagaagccg cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc
     4681 ctcggcattt ttctttgaca acagcaggat catgtcctga tcgcctttga tttcaccgct
     4741 catcagacgc atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt
     4801 ttcgaacggg atttcacata ccatcgaggt ttccagcgcc tgcgcgaagc tcgggtgatg
     4861 gccgctgccg atggcgtcaa atcccaccag gtcgcctgct aaatggaaac cagtgatttg
     4921 ctcgtcgcct tgctcagtga tggtataact tttaatcgta ccggagcgga tggcataaag
     4981 cgatttaagt tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg
     5041 ctcaatgata ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag
     5101 ctggctgatg ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat
     5161 aattcgcttt tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa
     5221 catcttttta gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta
     5281 gatgtaaata tatgtctatc tatttgaaaa cccttaagtt gttaagggta actttacata
     5341 aaagtgtgaa caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg
     5401 cgcaataacc actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg
     5461 ccaataaccc gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca
     5521 acggcacgcg ccgcgccagg acggcccaat tgctcagcca gttcgccgta atgcattacc
     5581 tgcccgcagg ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc
     5641 gtagcagtgg gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg
     5701 tcgcttaaac cgcctggatt ggtggcagaa atgcgctcat agccttcttt gcgataatgg
     5761 atgtccagca gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga
     5821 aattgctcat cgcaaatcac ccacagtgga cccagtggtg tggcaatttt ttcttcaagt
     5881 aatctcagca tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag
     5941 gcccacaaga taccacgcca gcaacatcag cagccatacc accaaaaaga taagcggata
     6001 gggcaagact aacgaatagt aagtacccag tttcgcgtct ggtttgtagc gttgcaggaa
     6061 tccaagaaac agtggaacaa aaggagatac tggcgctaaa ggcaatacgg atgagtcggc
     6121 aatacgaaag aggatttgcg caaatgccgg gtgaaagcca agtagcataa acattggtac
     6181 gaaaatgggg gccagaatcg accagattgc ggaaccgctg gcgataaaca tgcataagaa
     6241 agaggaaagc aacgccagac cgacaaacgc cgggatgccg ctaagccctg aactttccag
     6301 gatatcggtc agccccacgg cgatgaattt ccccatgttg ctccagttaa acatggcgac
     6361 aaactgggcg aggggaaaaa ccatcacgat aaatcccgcc atctctttca tcggttcaat
     6421 cattaaatgc ggtaaatccg cctgacgtcg aattgtgcgg gtagcgatgc cataagccag
     6481 cgagacaaca aagaaaaaaa gaatgatcag tggcacgata cctttaataa agggtgatgg
     6541 catcacggtg tgattaatcg gatcgcgcaa tatcccgttt tccgggatca ccatcagcgc
     6601 aatcgcagca ataaaaagta gcgatacgac acctgctatg cgtaaaccaa aacgctgact
     6661 ttcggtcaat gtctgcagtt tctcatcgct gtttccctgc cattgaccta accgtggctc
     6721 gatgattttg tcggttatca ggccgccaac aatcgtcagt acgactacgg agctggccat
     6781 aaaataccag ttatcaatta cactgacgtg catttgcgga ttgaacgcag ctgccgcttc
     6841 cgtgctgatc cccgacagca acacgtcggt tgtgacaatc agtaaattag ccgtaaagcc
     6901 gcaacctacg cctgcaatgg cagccagtaa acctgcaact ggatgcctgc ccaccgccag
     6961 aaaaatcagc gcacccatcg gtggcatgat cactaacgcc gcatcggaag aaatgtggct
     7021 gaaaaaagca ataaacagca ccatataact ggcgtagcgg gcattaacat gcgatgccat
     7081 tttaaccatt agtgctggca gtaagccgac gcgctccgcc agaccggcac ctaaaaccag
     7141 cgccaggatc gcaccaagtg gagcaaaacc gctaaagttt ttaataacat tgggtaaaaa
     7201 ccagtgtaat ccttccacac tgagcaggtt tttcaccacc accggcgtac catcggtcgg
     7261 gtttttcgca ctgacgccaa aggccgacaa aattgccgtc gtcaccatga gtacgataat
     7321 caaatagata aagagcaaaa aaggatgggg aaccttgtta ccaattcttt cgacccagcc
     7381 atagagcttc ccggattggg aggacgacgg tatggatgac atactcatgg gcattcctcg
     7441 gttgttgtgt ttgcgttgtt gttgttattt taaaggtgac ggtgtcacgt ttttcgggat
     7501 agggcagtga tacggttgcg tgtccgtaac ttgctgatgc tcttgttggc attcttgcaa
     7561 tagccctgaa tcaatgaaga gattgagtgt ggttgctgcc atagttttcg ccgccagcag
     7621 cattccttta tgagcaatag atgttcgccc ctggctaacc agttgccacg tatgtagggg
     7681 tgtaccgacg gcaaaacagg ggctgaaaca ctgggcaaca ggcagtttcc aactgacgtc
     7741 gccgacatca gtcgatgccg caagcacgtt atcggtggcg gcatatggag cgacttcatt
     7801 cgccagtacc gtttcacgat gacgtagtgc aaaaaccttg ccgttttcgc caccggttgc
     7861 ggcgatatta ttcagactgt tttgccgatc gttggaggta agcgtagcct gaatttgttt
     7921 cgcaaaagcc agttcttcgg agttccattc cggggtacca aaatgggata gggcctggta
     7981 catggcattt tctaaggtgc gattcgggag ataactggaa caggctttgt cgaagcggca
     8041 ttcaaccgtg gtttcggtca tcaatgccgc accttcggcg attttggcga cccgatcata
     8101 aatatgctgc acgtcggtca tttcgggggc gcggataaga taaagcactt ctgcctgcgc
     8161 ctggaccacg ttgggcgaga tcccgccact atttgtgatg gcatagtgta cgcgcgcttt
     8221 ttcaataata tgttcgttga ggaagttggt gccagtggtc atcaacgtta cggcatcaag
     8281 ggcgctgcgt cccaaatgag gggaattcgc ggcatgtgct gcgatccctt taaagcgcca
     8341 tgatgcctga atgtttgcca gcgtacgggt attgaacata ccggcaaagg cttccgggtg
     8401 ccaggtgagt gccgcatcca catcatcaaa taccccctcg cggaccatga acgttttacc
     8461 cgagccgcct tcttcgccag gacaaccata aaagcgcact gtgccgcctt gcccatattg
     8521 ttccagccat ttcttgatgg ctattgcagc ggcaaaggcg gcggttccca gcaaattgtg
     8581 tccgcaaccg tgaccatttt caccgggcgt cacggatgta ggttgcgcgc aacctgcttg
     8641 ctgacttaaa cctgccaggg cgtcatattc tcccagcagg gcgataaccg gtttgccttg
     8701 accaaacgaa gcaataaagg catttgggat attgcctacg ttgcgggtaa cggtgaagcc
     8761 tgcagattcc agcgccgaag ccagatgctc cgctgaccag aactcttcaa aacgtgtttc
     8821 tggatgatcc cagatttgat cggcaatatc ggtataacgt tggcgatcgg cttcaatcgc
     8881 atcgtcgata aaacgataga tttcctgcat cagatacctc gcgtccaggg aaaattgagc
     8941 gcggtgcgcg ccagcgtttc gacggcaata gcgagaacct gctcgtcaaa atcgaatttt
     9001 tcgttgtgat gacctgccgc cagctgtgtg ccaaacacca cgtaggaggc ttgcccttga
     9061 tgttgctgca cgcgggccat cattaatgtg gcatcttcgg aacccgcagg cgcttcaaca
     9121 cgttcaatgg cctgattgac ccccgcgacc tgagccgcct gactttgcaa ccatgcgacc
     9181 cattgcggcg aaggagaact ggcggtagct gcacccatca gacgagtttc aacgccgaca
     9241 ccatacatgg ttgctgcgcc ctgaatcgct tgctgagcac ggtcaaaaac atattgatta
     9301 atgacgtcgc tggccccgcg tgtttccact ttcagcaacg ccgaggcagg aacaacgtta
     9361 cgaccgcttc ctgcctgcat aacgcccacg tttactctgg aagctccttc gctgtgcggg
     9421 gcgattgcat gcagtgcaag agtggcttgt gctgccgcca acaaggcatt gtaaccgtct
     9481 tctggttttg cgcctgcgtg agcggcggta ccggtgaagt gcgcgtcaaa tttggtggtt
     9541 gccataaaat tatcactgcc gcacaccacg gtgcccgcag gtacgccagt gccaatgtgc
     9601 acggcagtaa aataatcaac atcatctacg acacctgcat cgaccatcgc ccgcgcgcca
     9661 cgcgtacctt cctctgcagg ctgaaaaatc agtttgatga cgccatgtag tccggactcg
     9721 aattgtttaa gggtatgcgc cagcccaagc ccaatggtgg tatgcccatc atgaccacag
     9781 gcatgcatca ttccggcgtt acatgacgca aaaccgtcgc ggtaggggcg atggctgaca
     9841 tcctgctctt cactgagatc cagcgcgtcc atatcgacac ggaaagccat caccggaccg
     9901 gggcgaccgg tatccagggt ggcgacgata cccgtgaaac caccttcaaa agccgcaatc
     9961 cattgtgcta gcgcaccctg ttgacgagcg cgctcgaatt cgcgttgtag agtgagttca
    10021 tcaggtagtc ccatccggct attttcatta actacttcgc ggcccagcgc cagtgagtag
    10081 ccgagctgct gcaattcttc cgcaacaagg gtggcagtgc ggaattccac ccagccagac
    10141 tctgcatagt gatgaaaatc acgtcgccag tgcgataatt ttggggcaag cgaattaaca
    10201 aattgattca aagactccat aacctttccc gtcatcagta aaaagtgtga cccggttcac
    10261 gtagcgatag tttttactta tcactaactg atttttcaca gttttaaccg ttcataaatt
    10321 accctgacac aatcatctgc attaaagtag atgccagttt ctttggtctg ataaataacg
    10381 gttatcggtg gcgtcatggc ttttcaggta aaaattcatc aaattcgggc ttttgttgaa
    10441 gtggctcgtc agggcagcat tcgcggagcg agccgaatgt tgaatatgtc gcaaccggca
    10501 ctgagtaaat ctattcagga gctggaagaa gggttagcgg cgcaactctt ttttcgccgt
    10561 agtaaaggcg tgacgttaac tgatgccggt gaaagttttt atcagcacgc cagtcttatt
    10621 cttgaagaac tgcgcgcagc ccaagaggat attcgccaac gacaagggca actggcaggg
    10681 cagattaata tcggcatggg ggccagtatt tcccgcagtc tgatgccagc tgtaatatct
    10741 cgttttcatc agcagcatcc gcaggtaaaa gtacgcatta tggaagggca actggtgtcg
    10801 atgattaatg aattgcgtca gggagaattg gatttcacca tcaataccta ttatcaggga
    10861 ccgtacgacc acgaatttac ttttgagaaa ttactggaaa agcaattcgc gatcttttgc
    10921 cgcccgggac accccgccat tggtgcccgt tcgatcaaac agttactgga ttacagctgg
    10981 acaatgccga cgccacacgg cagctactac aaacagttga gtgaattgct tgacgatcag
    11041 gcgcaaacgc cacaggtcgg tgtagtctgc gagacgttct cagcctgtat cagtctggtg
    11101 gcaaaaagcg attttctcag catactgcct gaagaaatgg gctgcgatcc cttgcacgga
    11161 caggggctgg tgatgttgcc ggttagcgaa attttaccga aagcggccta ttatttgatt
    11221 cagcggcgtg atagtcgcca gacaccactg accgcgtcat taatcacgca attccggcga
    11281 gaatgcggct atctgcaaag ttaaaactgc ataaaaaaat agagtctgtc gacatccgcc
    11341 agactctaca gtacacacag cagtgcatcc gcgtcttaaa tccggcattg tctcctctgc
    11401 gccggtgact gtgttataac ggttttaacc tttagttgcc aattttcttc agtgacagat
    11461 ttcacgaaaa tataagttac attattaata tcgtgaatga ataatcatgc ataagcattt
    11521 tgcttaaaat atcggcaata tttggaactt attactggaa ttttgggtaa tacgttgttg
    11581 gaccgacccg gtctggttat catatcgcgc tcttaattgc gggaggatgt aacatgaacc
    11641 ttgacgacaa atcgctgttt cttgacgcca tggaagatgt ccagccgctg aaacgtgcta
    11701 ccgatgtcca ctggcatcca acgcgtaacc aacgtgcgcc gcagcgtatc gacacgctgc
    11761 aacttgataa tttcctcacc accggatttc tcgacatcat cccgctaagt cagccgctgg
    11821 agtttcggcg ggaagggttg caacatgggg tgctggataa gctgcgcagt ggtaaatatc
    11881 cgcaacaggc gagcctgaat cttttgcgcc agccggtgga agagtgccgc aaaatgatgt
    11941 tcagtttcat tcaacaagcc atggcggatg gtttgcgtaa cgtgctgatt attcatggta
    12001 aagggcggga tgataaatcg catgccaata ttgtccgcag ctatgtggcg cgctggttga
    12061 ccgaatttga tgatgtccag gcatattgta ccgcgctacc gcatcatggc ggcagcgggg
    12121 cgtgttacgt cgcactacgt aaaacggcac aggcaaagca agaaaactgg gagcgccacg
    12181 ctaagcgcag tcgttgatct cgagacgcat ccgtggctta tgccgccagc acgcggttgc
    12241 gtccatcatt tttcgcccga tacaaagcat catcaacgcg tttaaacagt tcatcgatgc
    12301 tttcatttcc ttcgtgatgc gccacaccaa tgctgacggt aaagcgtggt aagcccgaaa
    12361 tactcacttt tgccacgctt acgcggatag tttcagccag cgaaagcgcg gtatccagtg
    12421 gggttcttgg tagtaataag acaaactctt cgcctcccca acgaaacacc aaatcgcctt
    12481 tgcgagcgca actttcgaga gtgcgggcga gggcgcataa cacctcatca cctttagaat
    12541 gcccatagag atcgttaatg tgtttaaaac gatctgtgtc gatgagcaat aagctgtaat
    12601 cctgagcgat ggcgaggtgc tgcatttggc ctggttccgt aatgtgataa aactgtcgcc
    12661 gattcagtaa tccggtcatc gcgtcatggt gagcagcatg ttccagctgc tcctccagcc
    12721 gtttttgctc agtaatatca tgcacaatac ataacatgag cttgtcgcca taaatttcaa
    12781 tcggtccggc ataggtctgc acatgacgag tcgaaccatc cgccagctta tgaataaaat
    12841 tcagaggttt atgaccaccg ggtaaatgcg agatttcatg catgataggc atgacgcgac
    12901 gcccgagcat atttatttcc caggtatgtt tctggcacat cgtttcatgg ttataaccat
    12961 agaaattgag cgcggcgagg ttagcatcga cgatttgtcc atctcgtgac gggtcaatca
    13021 acaacattgg tgcagagtta gtcagaaaaa agcgcgcata aaaaccttgt tttttgcgct
    13081 gataatttgc cgagcgactg gcttttaaac ccagcgttgc cggcgcttcg ataccttcga
    13141 aaataatcac cggttctgtt tctgtcagct ctcgcaaaac aagccgacag cttaatgctg
    13201 tttcctcttc tttacgctga acagtgagga tttcgataat atcgtgttgg ttttgcagat
    13261 cggagaggta tttcggcagt tctttttgtg aggagacgga atagggaccg gttcgtagct
    13321 gactaaacgt gaggtcttgc atcaacagtc gcgccgcgct gttggcataa attaactgtt
    13381 cctcaaaggg cgaaacgatc cagacaggac tggtgagtaa gtccagagta ttgaagttgt
    13441 gcgtaatcat tgagatccca ttatttttat caatttttgt tgctatccga
//
LOCUS       DBFIFS010000121        13375 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.121, whole genome shotgun sequence.
ACCESSION   DBFIFS010000121 DBFIFS010000000
VERSION     DBFIFS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.121"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            81..1139
                     /locus_tag="N7A07_003063"
     CDS             81..1139
                     /locus_tag="N7A07_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU2477686.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1149..2291
                     /locus_tag="N7A07_003064"
     CDS             1149..2291
                     /locus_tag="N7A07_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU2477687.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2284..3057
                     /locus_tag="N7A07_003065"
     CDS             2284..3057
                     /locus_tag="N7A07_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477688.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3059..4138
                     /locus_tag="N7A07_003066"
     CDS             3059..4138
                     /locus_tag="N7A07_003066"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU2477689.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4138..5094
                     /locus_tag="N7A07_003067"
     CDS             4138..5094
                     /locus_tag="N7A07_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU2477690.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5105..6313
                     /locus_tag="N7A07_003068"
     CDS             5105..6313
                     /locus_tag="N7A07_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU2477691.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6331..6798
                     /gene="terW"
                     /locus_tag="N7A07_003069"
     CDS             6331..6798
                     /gene="terW"
                     /locus_tag="N7A07_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU2477692.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7059..7388
                     /locus_tag="N7A07_003070"
     CDS             7059..7388
                     /locus_tag="N7A07_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2477693.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7375..7716
                     /gene="tnpB"
                     /locus_tag="N7A07_003071"
     CDS             7375..7716
                     /gene="tnpB"
                     /locus_tag="N7A07_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2477694.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7798..8628
                     /locus_tag="N7A07_003072"
                     /pseudo
     CDS             7798..8628
                     /locus_tag="N7A07_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8659..10272)
                     /locus_tag="N7A07_003073"
     CDS             complement(8659..10272)
                     /locus_tag="N7A07_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGU2477695.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10303..10653)
                     /gene="tnpB"
                     /locus_tag="N7A07_003074"
     CDS             complement(10303..10653)
                     /gene="tnpB"
                     /locus_tag="N7A07_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2477696.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10650..11075)
                     /locus_tag="N7A07_003075"
     CDS             complement(10650..11075)
                     /locus_tag="N7A07_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2477697.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11129..11880
                     /locus_tag="N7A07_003076"
                     /pseudo
     CDS             11129..11880
                     /locus_tag="N7A07_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         3214 a         3524 c         3494 g         3143 t
ORIGIN      
        1 atgattgaca tgtagcttag acactcatag actcacttct caatcataaa tttgcatggc
       61 gttccggata attaaaaaaa atgaacaaga aaatttggtt tatggaaggt ttatcctccc
      121 agcgagatat tattcagggg gtaaaatcat ttgcacaaaa aaataatttt gccattaccg
      181 tttttgcctc ccaccgtaac gaaagaaatg aaatcctttc cgttgccgat tattctttga
      241 ctgaacctga agatcctcaa aaacgtcttc agtttatcca ggaaaccatt cagacctacg
      301 gcatccacca tattcatact ggccgtaaca gccagtggtt tgaagaacac cgttcagcca
      361 ttgaaccgac cggtgccacc cttactaccg gtgcaacggg cgtcgactgg ttaactctgg
      421 ctgacgaaaa agttactttt gctcagttta tggagcaaaa gggtctcccg gtcgtaccat
      481 cctggcgggt gaatacgctg gcagaattaa agacacacct cgcggccccg ccgttcactg
      541 acagcccggt atgcgtgaag ccggtgacgg gtatctatgg catgggattc tggcgctttg
      601 atgacagtgc ttcgcctatg gccgtcttta atcatcccga acatcgtctg gtcagtccgc
      661 aacagtatat tgcagcagca tcagctgctg agtcgtttaa accccttgtt ttgatgccgt
      721 acctgccagg cccggaattt tccgtcgata tcctcgcgga taagggcgaa atactcgcag
      781 ccgtgggacg ccgtaaggaa ggggctatcc agtatctggt aaacgaagga agcgcctggg
      841 aactggcgtg tgactgcgcc cgtgttatga aggccgacgg gctggtgaat gttcagacgc
      901 gaaacgatgt gaatggcaac ccggtgctgc ttgaaaccaa catgcgtccg tcaggggggg
      961 tgggttatac ccttcacagc ggtgtgaacc ttcctgggtt atttgctgcc tttaagctcg
     1021 gtctgatgtc tgaagatatg gtacgccaga gcgctaaaaa caccttttct ccggttgcgg
     1081 tgagatccat tacggatgta attgcatacc cggaatcact ctctaacctt ctgaattaag
     1141 gcgtatttat gtctgatatc ctcgaagata tgatttatcg ccgtaccctc tcatgcggta
     1201 cgattcaggt aacccgtgac cagggtgaag tttcgctcga tgacctgttt gacatcgctg
     1261 aaagacgtaa cccgaaacgc gcctttctgt ttgtcagcaa agtactcggc aggcacattc
     1321 ccgtgccacc ctcagtcatg cggcaggcct acagacagct tgccagccag ttcccctcga
     1381 cactgacagg acccgtactg tttatcggta tggctgaaac cgccgttggg cttggggccg
     1441 gtgtatttga tgaagtgcgc caccagcatc gtgaatctgt ctatctgacc tctacccgtc
     1501 acccggttga tggcacgttg ctttgcgagt tcaaggaaga acacagccac gccaccgatc
     1561 atctgatcta tctgccagat gatgaagaga aaagacgtcg tgttaccaac gcacgaacgc
     1621 tggttttgat tgatgacgag gcaaccaccg gtaatacctt tattaacctg ctttctgccc
     1681 tgcgtaatac cggcaagctt caacatattg aacaggttat cgccgtaacg ctgaccgact
     1741 ggagtggcaa tgccctgtcg gagcgcaccc ccttgcccgt cacttcggtt tcgcttgtaa
     1801 gcggtcagtg gggatggacc ccattacctg atgcacctgt cccggacatg ccgaaagtca
     1861 acgtaacttc acgaggagaa tgggacatcc aggggaaaca gtcatggggc cgactgggga
     1921 tgctcgcacc tgcggccgat ctcggccatg aggtctccgt ccacaagggg gaacgtattc
     1981 tggttctcgg gaccggggaa ttcgtctggg agccgttcct gcttgctgaa cggctcgaag
     2041 ctgccggagc acaggcattt tatggatcga ccacccgctc ccctgtcgcc gttggttatg
     2101 ccattgagtc cgccatttcc tttacggata actacgggct gggcatcccc aattttgtct
     2161 ataacgtcgc ccaccagcag tttgaccgca ttcttgtgtg tactgagaca cccgcagaaa
     2221 gtattgacac gcagcttctt aaagcgctgg ctgaggttgc gcccgtcgtg gagattgtta
     2281 cctatgaata aacccgttgt tctgagcgac cttgacgaca cgctctttca gacccgtcgt
     2341 aaaatggtgg acgagctggc cctggagcca ttccgtaccg gtgccgttga tcgcaccctg
     2401 aatccacgaa gctttatgac ggaagaacag tccatgttgg tggactggct cctggagcag
     2461 gctgaactca tacccgttac cgcacgggga actgaagaaa tcagccgcgt acggatccct
     2521 ttccactcct gggcaatcac aacgcacggg gccgtcattc tcacgcctga aggcaaaccc
     2581 gacgaggagt ggaaggccca tatgctcggc cagctggctc cctaccagga aaagctgaca
     2641 tcgatgcagc gtctgatcac tgaaatgatg gacgcaaaag gaatcaatgc ctgggcaagg
     2701 ctgaacttcg aatacggtga aacggcggtt tatatggtga tgaaacaccg cgacagcacc
     2761 cgccttgacg agctcaatgc cattgcagat gagatagaaa cggtgtttcc gaccgagggc
     2821 ttctacatcc accgcaacag taataacgtg gcctggcttc ccaccccggt tgagaaaggg
     2881 ctggcagtca gctggctgct tgaaaaactt cgggctgaac gaggagtttt ccccgtaatt
     2941 ggtttgggcg acagcctgag cgatcatcgt tttatgaaac tgtgcagctg gtttggcatc
     3001 ccgcgtcaga gtcagtttgc agatgccatt tcacagcgaa tttttggaga aaattaaaat
     3061 gcagccacat gaaacattta ccggctcata ccagcccggt gacgtggaat ttctgctaaa
     3121 gccggttgtc attgagatga cgccggttga gaaaaaagaa gaactgattc agtccgggaa
     3181 gaaacattat tcggacatgc tcagccagga gccagcgcct acacaatggc atcttgattt
     3241 gtttcaccgg gcgctggatc ggggcgcaga gaggcttgca aaagaagtca cacagctcgc
     3301 tattgctctg gccgaacgct tcggtgatga gcccattgta ctggccagtc ttgtcagggc
     3361 tggcgttccg ctcggcgtta tgctgcacca ggccctgcgt gacatgggga aaacctcatg
     3421 gcattacggt atcagcatta tccgggatcg tggaattgac ggtgcagccc tcgacgtcat
     3481 tgaagagcgg catggtacca gcggtattgt ctttgttgac ggatggacag gtaaaggcgc
     3541 aattaccgga gagcttgtac gcgcactgaa agatcgcccg ggctatcctg agcagccgcg
     3601 actggttgtc ctggccgatc cctgtggctg ctcctggctt gcagccagtg atgatgactg
     3661 gctcattcct tttggcatca tgggggcgcc ggtatcaggc ctgatctccc ggtcagtatg
     3721 gtcctctgaa ggattacacg ggtgcatggt ttgcgagcat ctcagtgaat tcgaatgcag
     3781 ccggatgctt gccgataccg tcgctcattt ccgtaagaag ttaacaccgt catccctcgc
     3841 tcccctgagc tggaatacgg aatcagcccg ggtcttatgg cagacaagtc gcgacgttat
     3901 cgcgttcctg gccgatgaat ttaaagtgga cagcgtcaat cgtattaaac ccggtattgc
     3961 tgaagcaacc cgggctgtat tacgtcgggt accggaccat gtatttgtgc gttctattga
     4021 cgacccggac gttgccttgc ttgtagggct tgctcgtgaa aagggaatag ttgttacaga
     4081 aatgggggga accctcggcc agtatcgggc tgtaaccatt atcaagaagg tactctgatg
     4141 aaaaatcgtc tttctccctg gaatctggga gccacgctat acatgcctgc aacacgggaa
     4201 gatattgctg atgccgtcct gcacgggaaa atcccgggct tgcgctctct ggtgatttgc
     4261 ctggaggatg ctgtcagtga agcagacatc cccgtcgccc tcaaaaatct ggagcacctg
     4321 ctgcacgagc tcagtaacag catgagtagc ctgggtaaaa atgactggcc tctggtgttc
     4381 attcgccccc ggcatgccga aatgggcaga tggttaaaag cgcattatga tctttccgct
     4441 gtcgacgggt ttgtgctgcc taaatttacc ctcagttcgt tagctgaatg gtgggatatc
     4501 atggtcggga ctcacctgtg catgatgcca acgctggaaa cagaagatgt ctttgacgtg
     4561 gttcagatgc gcgagctggc tacccgtctg gaggaacatc cctgccacga ccgcattatt
     4621 gcgcttcgaa tcggcggcaa cgatcttatg aacgttgtgt cgcttcgccg tccccgggac
     4681 ctgacgctgt atgacagccc gatgggctac gtcatcaaaa tgctcgtttc ggtcttcggc
     4741 ccgcgtgatt ttgccctgac cgcgcctgta tgtgagcata ttgacgatca tgccgttatg
     4801 gccagagagc tggctcttga tatggcacac gggcttgttg ggaaaacggc cattcacccg
     4861 ggtcagatag aggtcattca aaacgcgctg atggtcactc agggtgagca ttctgacgct
     4921 ctgcggatcc tgaactctac ccaggccgtg tttaagtcgc agggagcaat gtgtgaaccc
     4981 gccacacatc gccgctgggc ggctggcatt ctggacaggg ctcgttttta tgggttacag
     5041 aacgagcaaa gcgctgatgg aatcagatta cttaccgtga cccagcatca ttaaggaaat
     5101 cataatgggg ttcaggttca gaaagtcgat caacattatt cctggcgttc gcctcaacct
     5161 gagtaacggt gcaccgagcc tgagtgtcgg gccgagaggt gcttccgttt cttttggtag
     5221 ccgggggacc tatgccaatc tgggcttgcc cggtaccggg ctgagttacc gtacccggct
     5281 tgaccgggcc gcgcgttcca gaggtgaaaa ccggacggca accgacccgg ggctcagaca
     5341 ggcgcttgag cagaaagccg ctgaactcat gtcagcggta accgcaatcc gtaatattca
     5401 cgagctgacg ccggatccaa aaacaggcat cagctgggca gagctggaag cagtatacct
     5461 gcataacaga acgtcgcctt ttcaggttcc ggcaccggtg cgtccagaaa agccagacta
     5521 ccttgtattg ccggaaaagc ctgccgagag cgaaggcatt agttttttgg gtaaatggtt
     5581 tgaatcggaa tcagctaaag ctgagcgcca cgccgaaaat cttcgccggt ggcagcagga
     5641 gctgattgat gtggagcgtg agaataccct tcgacagcac cggtaccagc aacaacggac
     5701 ggcctgggcc gaacagtatg caaactggaa gtttgaagct gaagaacatg aaaaacggct
     5761 cgccacggct caggcagatg cccggcagca gttccggaca gacgccgcgt ttttcgaatc
     5821 atacctggcg ggtgtgctgg cagaaactga atggccgcgt gaaacgcttg ttgcatttga
     5881 agtaaagccg gagctatcag cagtcctgct ggacgttgat ttagctgaga ttgaagattt
     5941 ccctgataag atttacggcg ttaatgcccg gggaacggag ctgacggaaa aagccatgac
     6001 gcaaaaagcc gtacgcgaaa actatgccca ccacgtccat ggctgcttgt tccgcctggt
     6061 cggtatcgtt ttacatacgc tacctttcga caacgtgatt gtgtcaggct ttacgcaacg
     6121 ggtcagtaag cggaccggct atctggagga tgagtatatc ctgtcctgca aatgcactcg
     6181 cagccagatg tcgtcagtaa attttgcagg cataaaacac attgatccgg ttgaagcgtt
     6241 aggcgatcaa ccggttattc gaaagatgag cagtaccttc atttttcagc ctattgaacc
     6301 actaaccctt taagcaggct aaatcttacg atgcaattaa ataccagaca ggcccggatt
     6361 tttaagcttg ccaacttact gggcaccggc aagcccgttt ccgctgccga cattataacc
     6421 agtctggaat gctctgagcc gacattaacc cgggccctca aagagctacg cgagtcatat
     6481 tctgcggaga tcaaatacag caaggcaggt cattcctatc atctcgttaa tccgggccag
     6541 ttggataaaa agaccctccg ccggatgaat gaagcgctcg cacagaatgc tgaactgaaa
     6601 accggtgagt ccacagggaa ggtcgtactc gataaggata aaaaaacagc cgtgtcatta
     6661 tcgctacgca tgcggattct aagaaaaatt gaccgtctgg ctgcgctgag tgactcgacc
     6721 cgaagtgaag cggtagagaa gctggctctg cactccgttg atgaactgat aaaagaatac
     6781 agcgctaaaa agtcctgacg ctaaatctca gaccggggtg aaaactccgg ttttgtcata
     6841 tttcacctct gtattggcgt gatgcaaaat ggcatctgaa tcgatcatgg tttggcatcc
     6901 atttccgatc actgtttggc atcacaatcg atcacgattt ggcatgcttc cgctcattga
     6961 ttggcatcct ggtgtcgtta cggggaagtc atcttcacag ggatcttcag tcagttagca
     7021 ttaccgacat ccattcacca acttaccgga gataatcaat gtcaaaaccc cgatggacgc
     7081 ttgatcagaa aaaacatcac gttgccgcat ggcgcgccag cggccttacc cgtgaacagt
     7141 actgtgaact ttacgacatt ccgtttaaat ccctgcgcca gtggccgcag gatgtcgcaa
     7201 aagccgaaaa acgagccaga gcaccagaaa ttattcctgt ttcagtatct ggctcttccg
     7261 gaatgactga tggcagacca ttgtcggatg agcctgtcac tctcttcctg cccggtggta
     7321 tccggatgtg ctgccagcct tctcagttaa ccgatgtttt ccgggctctt cgccatgctg
     7381 acgcctgata acgtattcct ggtcgtaaaa cccgtcgata tgcgccgggg tatcgacaca
     7441 ctgacacagt acgtccagaa cgaacttaac gctgcatggc atgacggtgc tgcttttgtc
     7501 ttcaccaata aagtccgctc ccgcataaaa gttctccggt gggacaaaca tggcgtatgg
     7561 ctgtgtaccc gacgactgca ccggggaagc ttccgctggc cccggaaagg tgatgccaca
     7621 tggcatctca cacaggatga gtttcactgg ctggtctttg gggttgactg gcaacaggtg
     7681 aaagggcatg acctggcaaa atgggtatac cagtaagaac ccgaagatgc tcagagcaac
     7741 gcaataactc aatgaaatat aatgaatatc catgcgtgat gtcatataat ccaccgcatg
     7801 gatatctcgc ttctcagtac caccagtgac cctgaacagc ttcgcgctct ggcaattgcc
     7861 atggtgcaaa aagtcatggc tgagaatgca gaacttcaga accgtatccg catcctggaa
     7921 gagcagatga agctcgcacg ccagcagcgt ttcggtaaaa aatgcgaatc gctggccggg
     7981 atgcagcgtt ccctgttcga agaggatgtt gacgcggata tcgcagaaat ctcggcgcat
     8041 ctggacaaac tgcttcctca gaccggggac gaagaaaaaa caacaacacg tcctgttcgt
     8101 aagcccttgc cttcacctct tccccgtgct gaaaaggtta tcccgccagc tgaggaacgt
     8161 tgtcctgact gtgatgcccc cctgcacttc attcgcgatg aagtcagcga gaagctggaa
     8221 tatattccgg cacaggtcgt ggtcaaccgc tatatccgcc cgcagtacag ctgtccctgc
     8281 tgtgagaaag tgttcagcgg aaaaatgccg gcacatatcc ttccgaaaag tgctgttgag
     8341 ccatcggtca tcgcacaggt tgtcatcagc aaatataccg accatctgcc cctgtatcgc
     8401 cagcagcaca ttttctcccg aatgggggtg gagctccccg tcagtacaat ggcggatatg
     8461 gtcggtgttg ctggcgctgc actggccccg ctggcaaaat tgttacgcca cgagcttctg
     8521 acacgggatg ttattcatgc agatgaaacg tccctgaggc tccttgatac ccggaaaggc
     8581 ggtaaatcct gctccggctg gctgtgggct tacgtcagcg gagaaagagt aagcgtcaac
     8641 ggagcaccgt attgacgctt atttattggt gagtactacg ttccatggca ggagttcatc
     8701 aactcggttg gaaggccatt ctggcagtac gctcaggata tggcgcagat acgcttccgg
     8761 atcgataccg ttcagacggc aggtgccgat cagcccgtac aacagtgctc cacgctcgcc
     8821 gccgtgatcg ctgccaaaga acataaagtt tttctttccg agacagactg cacgaagcgc
     8881 tctttccgca gcattattat ccgcctccgc cagaccgtca tcactgtaat aacagagggc
     8941 atcccactga ttcagtacat agctgcacgc ttcgcccagt ctggattttt tcgacagcgt
     9001 accattcttc tccaccatcc attcatgcag tgacgtcagt aacactttgc ttcgctgctg
     9061 cctgacggca agacgctctg actccggtaa tccccgtatt tcatcctcga tggcgtacag
     9121 ttcactgatt cgcttcaggg cttcttctgc cgtcgcactt ttgctgctga tgtatacatc
     9181 gtggattttt cgccgggcat gggcccagca cgcaacttct gtcagtgcac caccttcacg
     9241 ttctgcactg aacagccgat cgtaaccgct gaacgcatcc gcctgcagga taccccggaa
     9301 gggacgaagg tgttgtaccg gatgttttcc ctgcctgtct ggtgagtagg cgaaccagac
     9361 cgccggtggc tctgatgagc ccgcattccg gtcatcccgg acatacgtcc agatgcgtcc
     9421 tgtttttgcc ttttttctgc ccggtgccag cacttttact ggtgtgtcgt cagtgtgaac
     9481 cttgcgggtg ttcatcacgt aacggtacag ggtatcattc agcagagtca ttaactgaca
     9541 acatgcgtca acccagttgg agagtaatgc ccggctcagt tcgatgccct ggcgggcaaa
     9601 gatttcactc tgacgataca gtggcaggtg ttcgcagtat tttcccgtta acacgcgggc
     9661 aagtaatccg gagcccgcga tgccgcgctc tatcgggcgg gacggcgctg gcgcttcaac
     9721 tatacagtca cattttgtac aggctttttt tacccgaaca gtgcggatca ctttcagggc
     9781 gctactcacc agttccagct gctcagcact gacttccccc agataatcca gctcactgcc
     9841 acactccggg caacaacttt cttcaggctc cagacggtgt atttcacggg gaagatgtgc
     9901 cggtaacgga cgacgatgac gtgactgtcg caactggcgg ggaacctgcg ggtcatcctc
     9961 acgcccactg taacgatcgc tttcctgttc gcgttgtttc agttgggcct cagcctgttc
    10021 aacctcacgc tgcagttttt cagaacgggt accgaacaac atccggcgca gtttttctat
    10081 ctgggccctc agatgttcta tttcccgctc ctcctcttcg atcttttcct cggcacgtgc
    10141 cagtgcagag cgcaggaagg cctccgtctc ttcaaccaga ctcagttgct gatctttctg
    10201 acggagggct tcagcctgct cagagagcag cctttccagc tcagtgatac gaatgaggta
    10261 tttccgactc atgaccgttt ttataatccg gccatgacat ttttacaaca ttgtcagtgc
    10321 attaaggcgg gatgttttgg gctgacgcca gtccagttta tcgaggagca ttgccagctg
    10381 cgagcgggta atggatacct taccgtcacg caccgcaggc cagataaact ggccttcctc
    10441 cagacgtttg gtgaacaggc acagaccatc agcatcagcc cacagaattt taatcgtgtc
    10501 accccgtcgg ccgcgaaaga taaacaggtg accggagaag gggttctcat ccagcacatg
    10561 ttgtacctgt tctcccagac cgttgaagga tttacgcata tcagtaacgc cggcaaccag
    10621 ccagattcga gtgcctgatg ggagcgagat catcgtcccc tcccggtcag ttcacggatc
    10681 aacaccgtga gcagctctgg tgaaggattt tccagcgtca tgttaccgtg gcggaactca
    10741 actttacagg aactggcact gactgtgctt tgtgaaggag tggataaaag cggagtaaga
    10801 gccgccatag gctctttctg ctcatcaggc gttatctcaa caggtaataa ttcaacgcca
    10861 gcgccagaag aggttgttac cggaagacgc cgcgatatac gcctttcgtt ctgccagagc
    10921 ctgagccatt tgaacaggag gttatcattg atatcgtgtt cccgggcaat acgggcaaca
    10981 gaggctcctg gttgtgaagc cagtttaacc atttgaagtt taaactcatt tgaaaatgat
    11041 ctgcggggtt ctgaggataa tattttctgt tccataacag gtgtccacta gttgaaaagg
    11101 tgggcaccta cgttaccaat actggcttaa tggctacata cggcggtcag tttacgctta
    11161 cgagaaagaa gcggtccgcc ggtcgtctgt tttgactcgc agaccggacg ggccctcaga
    11221 tatcctgagg cctggctgca gggctggtgt ggcggtacgc tggtgagtga cggatacagc
    11281 gtgtacaaat ctctggcgga taatgacccc ggtattacat ctgcctgttg ctggagccac
    11341 gcaaggcgcg gtttcgcaaa tctgtataag gccagcaggg agccccgtgc agccatggcg
    11401 ctgagaaaaa tcgcgggtct ttatcgcatt gagaagttca tccgtgaacg tcctgtggaa
    11461 aaaatacggc aatggcgaca acggtattcc cggccgatag tgaatgacct gtttgcctgg
    11521 cctgaagagc aggagccgtg ctgtccgccc gacggaccac taaacaaagc gattaattat
    11581 atcctgaacc ggcgcgatga actgagctgc ttcctgagtg acggagccgt gcccctggac
    11641 aacaatatct gcgagcgtgc tatccgcccg gtggtcatgg ggcggaaagc atggttattc
    11701 gccggttcac tgatggccgg gaaccgcgcg gcacagataa tgagccttct ggaaacggca
    11761 aaacgtaatg gtctggagcc gcatgcctgg ctgacagacg ttctgacgcg tctaccggag
    11821 tggccggaag accgactgga agagttgctg ccccttgagg gatttacctt ctccgggtga
    11881 gtgatacctg ccatcaggtg ttcgtgcagc gggccataac ccgcagtcgg gagttgaacg
    11941 cctgacgaca ggaaatgagc cagagcagcg accacgcggg ctgacagtga gtcagaacct
    12001 gaatcagccc ggagccattc ccggggctgt acttcatcac ctgccatcag tacccgtgtc
    12061 agtacttcgc gacctgacgc aattacccga ctcagttctt cagaacccgc ctccgatacc
    12121 agcctggcgg tcgcccctgt gcaacaaaat aaatcaacca tcgtcagacg gtgatcaatc
    12181 gtatgcgctg ccagtggctc gcctgaaaaa tgcgtcaata tgagttcgcc ggaacaatac
    12241 ctcagttctt cagaacccgc ctccgatacc agcctggcgg tcgccccggc gcaacagaat
    12301 aaatcaacca tcgtcagact gcgatcattc gtatgcatcg ctgatgatgc gcccgaaaaa
    12361 cgcgtcaata tgagttcgcc ggaatattac tgacgacacg gtttgtagtt acgttccttc
    12421 ggtgagtgag tacctgtgcg tcagggtaac gcaatcagtt caggtttagc ggggtgtcaa
    12481 gtcggtttgg ctaaccgacc gcagaattaa ctgtaccttt aaaggtcgcc cccgatatat
    12541 ctgcctgtca gacatatcgc cgattcggta tctcactaca taaaactcct taaaaaacaa
    12601 tgcgattctg ttggtacgaa gctttcagta agtcagtccg gagaagcgcc agtacgatac
    12661 agaatgaggt tgaaaaaaac gttcacgtct ggtcacttct tcaaatgtta aaaaacatct
    12721 cgtaaagagc cagtttatgc gggttcgttg agtagacgtg cgagcgtcac tttgtcagct
    12781 ttaacgctga cgaaagtgac gcccgcacag cttcaaactc cacatgcaca cgcaagcgac
    12841 caaatttcaa atggtcttat gccgtgcgca cagtcataag aagcgcatgc ggaaagtcag
    12901 aaaatatctt aagacgcaga ttttaaaggc gtttttatgc cagtctgccg ggaatacaca
    12961 gtagtccggt tgtccggaag caccacggta atccgaagga aaaatcaacg tacctcaatc
    13021 accgtagtga tatccgttcc cagaggctaa acggatgtac caccataaag gcatcctctt
    13081 tcaggctacg aggatattgc aatcacccga aggcgtttct ctcagattgc tgaaacattg
    13141 gctggcagtc acccgaaggc gtccctctca gactgctgga acattggctg gtcgcaaaag
    13201 cgacagttaa tgcaaataca ggttaacaaa aagggaaatc tgccgtcaac ccaaccatat
    13261 gaacatgttg atttcattat gaatgtgatg ttctaatata aattaatcag taacttcata
    13321 aaaagaatat aagaaacaga taatgcatgt atttacacac atatcagcac aatga
//
LOCUS       DBFIFS010000122        13257 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.122, whole genome shotgun sequence.
ACCESSION   DBFIFS010000122 DBFIFS010000000
VERSION     DBFIFS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.122"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..332)
                     /gene="yhaJ"
                     /locus_tag="N7A07_003077"
     CDS             complement(<1..332)
                     /gene="yhaJ"
                     /locus_tag="N7A07_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU2477698.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPA"
     gene            437..1138
                     /locus_tag="N7A07_003078"
     CDS             437..1138
                     /locus_tag="N7A07_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU2477699.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1161..1325
                     /gene="yhaL"
                     /locus_tag="N7A07_003079"
     CDS             1161..1325
                     /gene="yhaL"
                     /locus_tag="N7A07_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU2477700.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1459..2769)
                     /gene="cyuA"
                     /locus_tag="N7A07_003080"
     CDS             complement(1459..2769)
                     /gene="cyuA"
                     /locus_tag="N7A07_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU2477701.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2797..4128)
                     /locus_tag="N7A07_003081"
     CDS             complement(2797..4128)
                     /locus_tag="N7A07_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2477702.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4403..5767)
                     /gene="tdcG"
                     /locus_tag="N7A07_003082"
     CDS             complement(4403..5767)
                     /gene="tdcG"
                     /locus_tag="N7A07_003082"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU2477703.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5839..6228)
                     /locus_tag="N7A07_003083"
     CDS             complement(5839..6228)
                     /locus_tag="N7A07_003083"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU2477704.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6242..8536)
                     /gene="tdcE"
                     /locus_tag="N7A07_003084"
     CDS             complement(6242..8536)
                     /gene="tdcE"
                     /locus_tag="N7A07_003084"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU2477705.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8570..9778)
                     /gene="tdcD"
                     /locus_tag="N7A07_003085"
     CDS             complement(8570..9778)
                     /gene="tdcD"
                     /locus_tag="N7A07_003085"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU2477706.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9804..11135)
                     /gene="tdcC"
                     /locus_tag="N7A07_003086"
     CDS             complement(9804..11135)
                     /gene="tdcC"
                     /locus_tag="N7A07_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU2477707.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11157..12146)
                     /gene="tdcB"
                     /locus_tag="N7A07_003087"
     CDS             complement(11157..12146)
                     /gene="tdcB"
                     /locus_tag="N7A07_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU2477708.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12245..13183)
                     /gene="tdcA"
                     /locus_tag="N7A07_003088"
     CDS             complement(12245..13183)
                     /gene="tdcA"
                     /locus_tag="N7A07_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU2477709.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3453 a         3563 c         3124 g         3117 t
ORIGIN      
        1 gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat gcgtttccca accgcgcgcg
       61 agagcttccg catcagtagt cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc
      121 aacaacatcc tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc
      181 acatccagct cttcttccag tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc
      241 agctcatccg ctgccgccgc aaaactgccc cggcgatcga tcgcatccat aacccgtagt
      301 gcttccagcg ttaatgccct ttctttggcc atttcgttct cattcaggaa atttgaacat
      361 accgggcaga atatctggct aacaatgtag cgtccagccc cttaccataa aaggaagtaa
      421 agagaggtca agaattatga ttactacccg aactgccagg cagtgtggac aagcagacta
      481 cggatggttg caggcccggt atactttttc ctttggacac tacttcgacc cgaaattgtt
      541 aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg ccgcctttca
      601 gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt ctggatggag aagcagagta
      661 tcgcgacagc gaaggcaatc atgttcaggc cagcgccggt gaggcgttgc tgctctctac
      721 ccagccgggt gtcagctaca gcgaacacaa tctcagcaaa gacaaaccgt taacacgaat
      781 gcagctttgg ctggacgcct gcccgcagcg agagaatccg ctgattcaaa aactggcgct
      841 taatatgggc aagcagcatt taatcgcctc gccagagggt gcgatgggaa gcctgcaatt
      901 acgccagcaa gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca
      961 gttgcatggg ccacgcacgt atttgcaatc gattcacggg aaatttcatg cgcttacgca
     1021 tcatgaagag aaagcggcgc tgacctgcgg tgatggggcg tttattcgtg acgaggctaa
     1081 cattacgctg gttgccgatt ccccactgcg cgctttgctg atagatttgc ctgtctagtt
     1141 gtttttacag ggagatgatg atgagtaaaa aatcggccaa aaagcgccag ccagtgaagc
     1201 ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa gagatgttga
     1261 gcgagctgga agccatcgtc gcggatgctg aaacgcgttt agccgaggat gaagctaccg
     1321 cgtaaagaca ggttgccgga tgcggcgaaa acgccttatc cggcctacaa aatccaacaa
     1381 cattaacgaa ttaaacaacc cgtaggccgg ataagatgcg ccagcatcgc atccggcacg
     1441 atacccaaaa cctggcgttt atctggcctt gctcgccata atctcgataa tctgccgatc
     1501 cgtttgctgc atcgaatggc ttgctaacgc acacaggttg gcaatcgact gctcaacatc
     1561 atgcgccacg atcccttcat tgccggtcac ggcggtatca tccagcgcca ttaacaccgc
     1621 tttccacgca gccgaagcac tggtcgaaac cttcatcgcg cagctgttcg acgcaccatc
     1681 gcaaatcatg ccgctgacat cgccgatcat actgctgatc gccatcgaga tggtttcata
     1741 acgcccatcc accagccatg ccatcccggc ggcggccccc attgctgcgg tcgttgcggc
     1801 acacagcgca gacaaacgcg gtaactggtt atggatgtaa attgcgctca aatgagaaag
     1861 catcagcgca cgcgccagcc gttcatcatc cgctccgaag tgttctgcta ccaccaccac
     1921 gggcattgtt gcggtgatcc cctggttacc cgagccggag ttactcattg ccggaagcgt
     1981 ggcgccgccc atacgcgcat cggatgccgc gctggtacga atcacaatgg atgaagagag
     2041 atctttcgcc agcaaaccgc gctcgcactg tttttccagc gtcgcgccaa tatgcagccc
     2101 ccacttaccg ctcaaacctt cctgcgataa cgcacaattt agcttcgcgg aatcgagaat
     2161 aaagcggatc gccgcaaacg ggacttcatt gacgaacttc aggatctcag ccagcgtcgt
     2221 tctggaaagc accgatagcg gagactcttg ctcgccctct gccacacacg cctgctgggt
     2281 aaacaccaca ccatcgtgcg tctcaatatg cacaatgttg gtatgcccgc cgacgatagt
     2341 gacacacgcc cacttctcac cgttccagac tttggcgcgt gagaagagga tttcattgca
     2401 aggttcctgg atcttaacgg agactttccc cgccgccagc agtgctttgg catcggcaat
     2461 tgcctgcgca gttgcgtctt tcagcacttc cagcccggcg ttggcatttc cacctaacgc
     2521 ccccagcgcc gccgcaatcg gcagccccac cattcccgtg ccgggaacgg tgacgcccag
     2581 accgttcttc atcagatttg gcgaaaccca ggcttctaca cgttcaaccg gaccttccag
     2641 ttctgccgca gcaaccgccg ccgccagcgc cagtgaaatc ggttcagtac atcccagcgc
     2701 cggttttact tcctcctgaa cggcgaggat gtaacgctgc cataacggat ttaaagtcga
     2761 atcaaacata ttaaaaacct taaaatttca ggtaaatcaa gaaaatgcca ggaacggaga
     2821 aacacaaagc aacaaaccag tgacgataat caggtacaga gacatccctt tgtatttgtg
     2881 caatgccggt actttgtaaa ccagccacgc cgggatcagg caccctacca agccgaaaat
     2941 cgggctacag atagaggtga agctcaacac cggtgcgttc agtacgatgg cgctccaggc
     3001 cagcaaaatg gcgaaaatca tgatgccgcg ctgaacaaga ttttcgttaa tcttctcggc
     3061 aggcatcttg cgacgcagga tgttcattac gatcccttgc gttgcttcgc gaaaacctaa
     3121 atagacgcca aagaatgcag tcatgacagc aaagatattg agaatgacgc tgaccacttt
     3181 cacccacgct gcgccgtcac cgctaataaa ctgtgcggca atcgccagcg cggaaatatt
     3241 ctgctcgtag gcttttaccg cttcgtcatg tcccatcgcc agcgtgaacg acacggcgta
     3301 gaaaaagacg atgataaaca aaatgccaaa cgcgatattc attgcccgca atgctttatg
     3361 ccgcgctact tcaatcgatt tttcccgcga gcgataagag atcaccatcg ggcttaacgt
     3421 ctggataaac agaatcgacg tcagggtaaa cggcagcgta ataatagcgt ttttcaccag
     3481 cagccccagc ggcggtagcg aaccgacgtt atacagatgc cacattccca ccatcgacac
     3541 gcccagcgcc gcgaccacca gcagcttggt cagcaccatg ccagtcgaaa ttttgaataa
     3601 caatttctcg ccgcgtgaag agatcgccac cagaatgcaa atcagtacca gaccataaaa
     3661 gggactgtct gacagcaacc cttccgtcac gccgaaggta tgcaggtagg aagcactatc
     3721 gttggtgatg gcggtggaat agacgaacat ccaaatcacc agcattacga aatagagtgc
     3781 acctaacagg atgccccagt ttttacctaa ataaccgcta atgacgctcg ggtaatcttt
     3841 acattctggt gattctgcca gcgtattaat aaacaaccgc tgaaacagat acattgccgg
     3901 gtaaccaatc accgatgaga gcaaaaatac ccacaatccc atcaaaccga cctgcaccgg
     3961 gagaaaaaca atccccgcgc caatcgccat cccgatactc ataatcaccc agccggtgtc
     4021 agtactgtcg aatttgatcg cttctcgcca ctcgctctca ctcattccag cacgacccgc
     4081 cggggtcgaa gcgtctgcaa tgacgccttt attcgatgca atttccataa tttctcgctc
     4141 aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat gcggtctacg
     4201 tttttgcagg cgagttatta gaataaagaa atgatacgcg cgaggtcaga gaaaatcttc
     4261 acaatcaaga ctttgaaata acggagatgg ataagaattt tctactaaat taatcgcaag
     4321 agaatattca ctctctattt gcggcagatc acaaaaaaaa ggtgcacatt tgtgcaccca
     4381 aggatgaaag ctgacagcaa tgtcagccgc agaccacttt aatggccagt ccgccgcgtg
     4441 atgtttcgcg gtatttatcg ttcatatctt tgccggtttc atacatcgtc tcgatcactt
     4501 tatcgagtga aacacgcggt gccgaggtgc ggcgcatcgc catccgcgcg gcgtttactg
     4561 ctttcacggc attaatggca ttacgttcaa tgcacgggat ttgtacctgt ccggcaaccg
     4621 gatcgcaggt cagtccaagg ttatgctcca tcgcgatttc cgccgcattg catacctgcg
     4681 ccggactgcc gcccagtagt tcagttaacc ctgccgccgc cattgaacag gccacgccaa
     4741 tctccccctg acagccgacc tccgcgccag agatggaggc gttcatttta tacagcgcgc
     4801 caatagcccc ggctgcaaga aaatagcggg caattgaccg ctcgtttacc ggacgacgga
     4861 acttatcgta ataagccagc actgccggaa taatgccgca cgcaccgtta gtcggtgccg
     4921 ttaccacgcg cccgccagct gcgttttctt cactaaccgc cagcgcgtac atgttgatcc
     4981 agtcgatgac attcatcgga tcgttagaga tgttatcgct ggaaaccagc tgacgacgca
     5041 gcgctacggc acggcgcggc acattgagcg gaccaggcag cacgccttca gtgttcatcc
     5101 cgcgttcaat accgtcatgc atcacttgcc agatacgggc aaaaccggcg tcaatttccg
     5161 ctttactgcg cagcgctagc tcgttgtgca tcatcaggcc agaaatagac aggccgttgt
     5221 aatcacacat tttcagcagt tcacctgctg agtggaaatc gtaaggtacg ggtgtttcga
     5281 catcgtgcga caggccgaag tgttcttctt cgacaataaa cccgccgccg acagagtaat
     5341 aggttttact taatagctct tcctgtgcct tccaggcagt gatccgcatt ccgttctcat
     5401 ggcgaggcaa catttcggga tggaagataa tgttctttgc tacaggaaaa tcaacaatat
     5461 gcgcaccaga tgccactggc agccgcccgc tgcgcgttac taactctata aatgcaggga
     5521 tctcatcaat gacaacatcc tgcggactgt ttcctgccag tcccatgatg atggcgacat
     5581 ccgtggcatg gcctttgccc gtcagtgaca acgacccgta cagatcgacc acaatatggc
     5641 tcgtcgcggt taataagccg ctactttcca gccgatcaat aaaacttttt ccggcattca
     5701 ttggccccac ggtatgcgaa ctggagggac caatcccaat tttgaaaata tcgaatgcac
     5761 taatcatatc cacaccctcg gattgccgtt cagtgaagtg gagcggaacg accttacgac
     5821 cgtcccgctc acgaggcttt acgcactacg tactgcgata gcttcaattt ccagcttcac
     5881 atctttcggc aaacgcgcga cctgtacaca gctccgggtc gggtaggtcg cctgatgctc
     5941 atcgaagaat tgcttataga cttcgttgat ggtggcaaaa tcattcagat cggtgatgaa
     6001 cacggtcatc ttgatgatat cgcccacgct cagcccggca gcaaccacga tcgctttgac
     6061 gttttcgagg cttaaacgcg cctgatcttg cacatcagcc gggatctcac cggtctgtgg
     6121 gcaaaccgga atttgaccgg aggtgaagac catgctgcct aaatcaacgc cttgaacata
     6181 agggccgatt gcgcctgggg cacgttgcgt ttcgataatc tttttcatac atcctccggc
     6241 gtcagagcgc ctgggtaaag gtacgtgaaa taacatcctg ctgctgttca cgggtcagtg
     6301 cgttgaagcg cacggcgtag ccagagacac ggattgtcag gttaggatat ttttccgggt
     6361 gctcgatggc atccagcagc atttcccgat tcattacgtt gacgttgagg tgttgaccgc
     6421 cttcgacatc cgcttcgtgg tggaaatacc catccagcag gccgacaagg ttggttttac
     6481 gtactggatc ttctttgccc agcgccgcag ggacgattga gaaggtgtac gagatcccat
     6541 ctttggcgta ggtgaacggc agtttcgcca ccgacgtcaa tgaggccacg gcacctttgc
     6601 ggtcacgacc atgcatcggg ttagcgcccg gcgcgaacgg tgttccggcg cgacgaccgt
     6661 ccggcgtgtt accggttttc tggccgtaca ccacgttaga agtgatagtc agaatcgact
     6721 gggtagggac ggcgttgcga taggttggca gcgctttaat tttcttcata aaacgttcaa
     6781 ccaggtcgca ggcaatgctg tctacgcgct cgtcgttgtt gccgtactgc ggatattcac
     6841 cgtcgatttc aaagtccacc gccaggccgt ttttgtcacg gattggtttc acgcgggcat
     6901 atttgatcgc agacagggag tccgttgcca ccgacaggcc cgcgatgccg cacgccatgg
     6961 tgcgatagac atcacgatcg tgcagcgcca tcagcgaagc ttcgtagctg tacttgtcgt
     7021 gcatgtagtg aatgatattc agcgcgctga tgtactgcac cgccagccag tccatgaagt
     7081 gatcgaggct gtccatcact ttgtcgtaat ccagcacatc gtccatcaac ggtgccgttt
     7141 tcggcccgac ctgaatcttc agcttctcgt ccaccccgcc gttaattgcg tacagcagtg
     7201 ttttcgccag gttagcgcgt gcaccaaaga actgcatttg cttaccaatc accattgggc
     7261 tgacgcagca ggcaatcgcg taatcgtcgc tgttgaagtc agtacgcatc agatcgtcgt
     7321 tttcatactg caaggaagag gtgacgatcg acacctgcgc ggcatatttt ttgaaggcaa
     7381 tcggtaattc ttccgaccaa agaatggtca ggttaggttc cggtgcaggc cccatagtgt
     7441 gcagggtgtg caaatagcgg aaggagtttt tggtcaccag cgtacgaccg tccagcccca
     7501 tcccgccgat cacttccgtc gcccagattg ggtcgccgga gaacagcgaa tcaaattccg
     7561 gcgtacgcaa gaagcgcacc atacggatct tcatgatgaa gtgatcgatc agttcctgtg
     7621 cctgctgctc attgagtacg ccagctttaa agtcgcgctc aatgtagata tccaggaacg
     7681 atgccgtgcg gcccagcgac atcgcgccgc cgttctgtga tttcactgcc gccagataag
     7741 cgaagtagag ccactgcacc gcttcctgcg cattctgcgc cgggcgagag atatcaaagc
     7801 catatttcgc cgccatttcc tgaatctgca acagcgcatg acgatgctct gccagctcct
     7861 cacgcagacg gatggtggct tccagatcct cgcctttttc cagacgagac tggagatcgg
     7921 caaattgcag ttcgcgttca cgcaccagat aactgatgcc atacagcgct acgcggcgat
     7981 agtcaccgat aatgcgccca cggccatagc catctggtaa accggtcagc acgccagatt
     8041 tacggcagcg cagcatatcc ggtgagtaaa catcaaatac gccctggtta tgggttttac
     8101 gcagatcggt aaacagatat tcaaattcac tgtccatttc acgaccatag gcgtggaatg
     8161 aacttttaat catattaatg ccaccgaacg ggtgtaacgc acgtttcaac ggcgcatccg
     8221 tttgcaggcc aacaattttt tccagcggct ggttaatata tcccgcatca tgagcggtaa
     8281 ttgtggtggc aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt tcaatacgaa
     8341 tgccttccat caccttttcc cacaattccg tggtggctgg cgtcgcttca gcgaggaaag
     8401 attcatcgcc ttcatacggt gtatagttat gttgaataaa atcgcggaca ttaatttcgt
     8461 ttttccagtc cgtaccttta aagccaagcc atgcgtcggc gtacagctta tcgctggtat
     8521 caatatctac cttcatgaaa aataatctct ctacaatact tcaactaaat tatgcaaatt
     8581 ctgcgggtgc gttaactttg cctaaatgaa tggcatccaa agcaatcatt ttttcttcgt
     8641 tggtcggaat tacggcgcaa atgacacgcg cattttcact ggaaacaatt cgctcgccaa
     8701 aagagttaga tcgattattc atttctgtat caatctctac gcctaataca gccaaatgtt
     8761 ccatgaccag acgacgaatt aaacttgaat tctctcctat tccgccggtg aatataattc
     8821 catccaggcg acgtaatgaa gctgcgtgtc cggcaatatg acgggcaatt cggtgaacaa
     8881 aggttttaat tgccagctgc gcgcgttcgt gaccttcatg ccaggctttt tccagaacac
     8941 gtaaatccga agaaagaccg gaaataccta ataatcccga ctctttattc actacgcgtt
     9001 ccaggtcacc caggctctgg ttggtttggc tggcgaccca ggacatcgca ccaaagtcga
     9061 catcgccact gcgggtaccc atcatcaagc cttccagcgg cgtcattccc attgaggtgt
     9121 caacactctg accgttgcga actgcgcaga ttgacgcgcc attgccaaga tgcgccacaa
     9181 ccaggccgga gtcatcttcc gccagattca gcaaagaatg tgcgcgcagg gaaacatagc
     9241 ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa ctcttcataa tatttccacg
     9301 gcaggccgta taaataagct tccggagcca tcgtctggtg gaaactggta tcaaataccg
     9361 ccacctgagt tacgcccgga aataattgct gcgccgattc aataccactt aaattggcgt
     9421 aattatgcag gggtgccagt ggagaaacgc gacggatatt atcaatgact tcatcggtaa
     9481 taatggcgga ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca attaaggcca
     9541 cgctgtcatt taaattccgt ttttccagtt caaatgcaat tgccttcaat gcaccttcgt
     9601 agctgtggtg agccagcggt gctggctctc ccccatttac ggataagaat gcattttccg
     9661 agttaatacc gtcggcaata cctgacatta atacttcaca gtcgctggca tcgagtacgg
     9721 aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat tcattcatct
     9781 cttttctcat cctgagttac ggattaaaac agtttatata cgatgttcag gatggtcagc
     9841 agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc cagagacggc
     9901 gctttacgga tggcatacat cggcaacagg cacagcaggg atgcgataat cggtgcgccc
     9961 atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca ggtggagccc
    10021 atgatgaaga tcatgctgat agtgttcagt ttacccagcg atactttagt tttgtcgcct
    10081 ttataaccaa acttcaggac caggccattc agaccttcca gcgttcccag atagtgaccg
    10141 aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc cagtgtaatc
    10201 gcgaacgttg ttttggtacc ggtcatggac gcaaagtggt tagccagata agaaagcact
    10261 ggaatattct gcgctttggc ttccgccatg ttggccggag acagcgtaaa caggcagcta
    10321 aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat gatttgggaa
    10381 catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg tttagaaacc
    10441 acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga aatccccagc
    10501 cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag gtcaacctgg
    10561 tcgataactg cagagttcca gtaagggatc agcgacaaag aaatcagcac caggctggcg
    10621 ataaacggcc ataccaggta gctcatcact ttaaccatca gatccttacc aaaccagatg
    10681 acgaaagcca tcagcagcaa caggaacagc gccacaaagc cgcgattcag cggtgcaaag
    10741 ccgagctggt tttcccagaa cgtcataaag gtattggtaa tagtaacgcc ataaatccac
    10801 agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc agttttacca
    10861 aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt tagagccaga aagacacaga
    10921 cgcgccagcg cccggtggca ataaaatgcg atggggtatg ccaataccaa catcagaaga
    10981 atcgggatca gtccgccaaa acctgcgcgg atagggaaga acagcacccc ggcgccgatt
    11041 gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt tacgccagga cgattgtttt
    11101 gtctggctgg atacaatgct atctgaagta ctcatatcct atcctcaacg aattaattaa
    11161 gcgtcaacga aaccggtgat ttgagagacg cgagaaagat cgatattgcc gccggaaata
    11221 atactgacgg tttttctgtt ttgaatatat tggtctaatt taccgcttaa taatgcagca
    11281 catgccagag cgcctgcgcc ttcggtgacg actttattgc gctgaattaa ggcaatcata
    11341 ctgtttctga tttcgtcttc gctgaccagc acgatgtcat cgactaattc acgaacgatt
    11401 tcgtaagtta aattacccgg gcgggagaca tcacaaccat ccgccagggt gccggtagtt
    11461 cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac gttttcagac
    11521 tgtacgccaa taacacgaat ggtcgggtta atagatttaa ttgccaccgc aataccagca
    11581 attaaaccgc caccaccaat tggcacaatc acgttatcga catcatagag atcttccata
    11641 atttccaggc caatcgttcc ctgaccagca atcactttcg gatcatcgta aggtgggata
    11701 aaaatacggc cttccatttc gacaatttcg ctcactttag cgatagtgtc gttgaagtta
    11761 tcaccatgca gaacgacttc tgcggagtag tcgcacgttg ccgctacttt ggatttcggc
    11821 gcgccttttg gcatcaccac tttaccgtcg atacccagca tcgcgcagga gagcgaaacc
    11881 ccttgcgcat ggttgcccgc agaacaggcc accacgcctt tgcgtttttc cgcatcagtc
    11941 agtgaactta atttattaaa tgcgccacga attttaaatg aacccgtacg ctgcatattt
    12001 tcaaacttca ggaatatttc acctttgcaa cgttcactaa aatagttgga gcgaggcatg
    12061 cctgttttat aaattcgccc agccagtcgt tgtttcgctt caataatgtc atcaatagca
    12121 accggcagat cgtatgtaat atgcattata aaacctcttc gcctgaatta aatatgtagg
    12181 taaccgtaac cgacacctgc aagacagaca ggtggattat ttatgtttaa ataaaaacaa
    12241 ataactaacc aacttctatt aattgccttc gtctacaccc attataagat gaatactctt
    12301 tggctaattc caccaaaacc gatgctgctt ttttaatacg ataatttttc gaccataccg
    12361 cggcatattg tgccacaggt aatgtttctt caaccggaat agtaataaat tgattagaac
    12421 caaaaggaga cgtcatatca caaggaatta cagttaagaa atcagcattg agaacaagat
    12481 tataaattgt cacgactgag tcggttttaa cgatgttttc aatactgatg ccatttcttt
    12541 gtaacgtagt aagcagttcg ctgtagtacc ccatattagt ttgtggcaac acccactgtt
    12601 cgttcttcaa cgactccagc gtggtggtgc cggtgcatgt tcgggactta ctggctacca
    12661 gcacaaactc ggactcgaac agcggctcaa catgtaaatc ctgaagcttc atttctgcgc
    12721 ttaacgtacc aatcgcaaaa tcaagtcgac cgtcgcggat tgccggtaag aacgaagaca
    12781 gttgcgcttc atacatagaa acctgcgctt tcgggaacac ctctttgaac ttgttgatca
    12841 tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact tccaccaccg
    12901 cctcagaaga cataccgctt atctcattaa ccatattttt catttcacgg gtaatggatt
    12961 cggaacgaga gagtaacaat tgaccggcag gtgttaatgt tactccggta tttttccgca
    13021 ccactaattc cacaccaaaa taatcttcaa tatcgttaat gattttactg acggccggtt
    13081 gagttaaccc taattctttt gcagccgagc cgatagaacc acttctaatg acttcctgaa
    13141 agactaccag gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc tacgttaatt
    13201 acctcattga cggcatgaag tgtatcaaaa tgaaatgaac aggatatgtg cgaccac
//
LOCUS       DBFIFS010000123        13180 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.123, whole genome shotgun sequence.
ACCESSION   DBFIFS010000123 DBFIFS010000000
VERSION     DBFIFS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.123"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            234..1154
                     /gene="cbpA"
                     /locus_tag="N7A07_003089"
     CDS             234..1154
                     /gene="cbpA"
                     /locus_tag="N7A07_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU2477710.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1154..1459
                     /gene="cbpM"
                     /locus_tag="N7A07_003090"
     CDS             1154..1459
                     /gene="cbpM"
                     /locus_tag="N7A07_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU2477711.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1613..2212)
                     /gene="torD"
                     /locus_tag="N7A07_003091"
     CDS             complement(1613..2212)
                     /gene="torD"
                     /locus_tag="N7A07_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU2477712.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2209..4755)
                     /gene="torA"
                     /locus_tag="N7A07_003092"
     CDS             complement(2209..4755)
                     /gene="torA"
                     /locus_tag="N7A07_003092"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU2477713.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4755..5927)
                     /gene="torC"
                     /locus_tag="N7A07_003093"
     CDS             complement(4755..5927)
                     /gene="torC"
                     /locus_tag="N7A07_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU2477714.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6057..6749
                     /gene="torR"
                     /locus_tag="N7A07_003094"
     CDS             6057..6749
                     /gene="torR"
                     /locus_tag="N7A07_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU2477715.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6722..7750)
                     /gene="torT"
                     /locus_tag="N7A07_003095"
     CDS             complement(6722..7750)
                     /gene="torT"
                     /locus_tag="N7A07_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU2477716.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7833..10577
                     /gene="torS"
                     /locus_tag="N7A07_003096"
     CDS             7833..10577
                     /gene="torS"
                     /locus_tag="N7A07_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU2477717.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10649..11722
                     /gene="yccM"
                     /locus_tag="N7A07_003097"
     CDS             10649..11722
                     /gene="yccM"
                     /locus_tag="N7A07_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU2477718.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11771..11905)
                     /locus_tag="N7A07_003098"
     CDS             complement(11771..11905)
                     /locus_tag="N7A07_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU2477719.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(11933..12163)
                     /gene="ymcE"
                     /locus_tag="N7A07_003099"
     CDS             complement(11933..12163)
                     /gene="ymcE"
                     /locus_tag="N7A07_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU2477720.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12138..12326)
                     /gene="ymcF"
                     /locus_tag="N7A07_003100"
     CDS             complement(12138..12326)
                     /gene="ymcF"
                     /locus_tag="N7A07_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU2477721.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12337..12549)
                     /gene="cspG"
                     /locus_tag="N7A07_003101"
     CDS             complement(12337..12549)
                     /gene="cspG"
                     /locus_tag="N7A07_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU2477722.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12835..13047
                     /gene="cspH"
                     /locus_tag="N7A07_003102"
     CDS             12835..13047
                     /gene="cspH"
                     /locus_tag="N7A07_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU2477723.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         3178 a         3459 c         3347 g         3196 t
ORIGIN      
        1 taataacgca ctagtaaaac ttaaagtgcc tgtttcaact atcaaaaatc gctcaccctt
       61 tttcacctgt ttaaaatatg ttcagcgccc caccttgagg cgacctcctc tccgcgatga
      121 tttcaataac atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg
      181 agttacctta caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat
      241 taaaggatta ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga
      301 ccgcctatcg tcgacttgcc cgcaaatacc atcctgatgt cagcaaagaa ccggatgccg
      361 aagcccgctt caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg
      421 ctgagtatga tcagatgtgg caacatcgca acgatccgca atttagccgt cagttccagc
      481 atggcgacgg tcagagtttt aacgccgaag attttgacga tatcttctcg tcaattttcg
      541 gtcagcatgc ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg
      601 aagtggcggt attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc
      661 tgccggttta taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga
      721 agatcccggc aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg
      781 gcgaaaacgg cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc
      841 tgtttgatat tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg
      901 cgctgggtgc taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc
      961 ctggcagcca ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac
     1021 agaccggcga tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca
     1081 ctgccgcgct gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt
     1141 gggggaaagc ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca
     1201 taccggcatc tctgaagagg aattgaatga aattgtcggt ttgggggtgg ttgaaccgcg
     1261 tgagatccag gaaacaacct gggtatttga cgaccatgcc gccatcgtgg tgcaacgcgc
     1321 ggtgcgcctg cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt
     1381 aatggatgat attgcgcacc tgaagcagga aaaccgcctg ctgcgccagc ggctttcccg
     1441 gtttgtagct catccgtgat gtgtgtttgc cggatgtgat gacgcatctt agcctgcctg
     1501 caaagagtac ttttcctgtg ggccggataa ggcgtgttca cgccgcatcc ggcggcggtg
     1561 ccctaatgcc tgatgcgacg ctaaacgcgt cttatcaggc taacaaacga cgttatctgt
     1621 tttggtggtc gccctcaact aacaccagca ataactggct tagtgccgcg taaaaaccaa
     1681 agctgtcata ctgatggcaa cgcgcaacaa actctggtaa ccattgccgc aacgccgtga
     1741 gtgttttttg ccgtaaactg tcgattcttc gcgcaggaac cgtcccctct cccagcgaaa
     1801 aatgcagatg actcaacaac tcgagataga tcgccagatg atctgtcggt tcgttgaaat
     1861 tgccgctggt ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt
     1921 tgtaggccga tgcatacggc agcgccgctt gtttgtcagt catcagaaac aggccgcaaa
     1981 agtcagcggc cagttccaga cgggcatcgt cacgtactgt cagagtggca acacggtttt
     2041 ccagctcatc caccgccgca gtgagcggcg gttcgctttt cagcaacgaa aaccattcag
     2101 ccatctgcgc actggcgatt tgcgtcagtt gttcatcgtc cagttcacgg gagaacaact
     2161 gcgccagcca ggcgtaaaca caggctatct gttgtgctgt cagcgtggtc ataatttcac
     2221 ctgcgacgcg ggaacatatt cgcactgcgc caccatctcc acggggccgt taaacgccgt
     2281 tacctgctcc actgttccgt tgcacttctc aatttccacc agcgtagtgt gcgcactggt
     2341 cgcctgcgcc agctgcgaag taccgatgtc aatggtcaac acgttggggt taccgtattt
     2401 gcacagcgca ccaggctcgc cgcctttatc tggatcgtgc catgcccctt cgtgaattcg
     2461 cgccacgccg ggtgcatagc ggtcagaaac tactgcccct gccaacacct gaccgcgagc
     2521 gttaaagacg cgtaccacat caccgttacg aataccgcgc gcgctggcat cctgcgggtt
     2581 aatgaacact ggctctttac ccgctaccgt atattgctga cgcagagttt ccgactcaca
     2641 taactgcgag tgaagtcgga aatccggatg cacagattgc agatgcaacg gatacgtctg
     2701 cgagccgggc ccaccgtggg agcgttcgat tttctcaaac cacatcggat gcccctgaca
     2761 atcgtcgtag ttcatatcgg cgatggtttt cgaatagatc tcaatcaggc cactcggcgt
     2821 gcccagcggt tcgagatctg gatcttcgcg gaatgcctgg tggcgaacaa acatctgcgg
     2881 atggtcaaac tcgacgtact ctttgttatt ccagaagtca tcaaacgctg gcaaatgaac
     2941 gccgcgtcct ttgccttgct gaacaccttc ctgccagatg cgtttcagcc agcccatttc
     3001 gtccagccct tcggtaaagg cttcttcgcg attaaagcgg cggcacagct cgcggaaaat
     3061 atcaaagtcg ttgcgcgcct cgaactgcgg cggcacgacc tgtttcatgg cgataatgcc
     3121 acggttggag tggttgccgt actggtcgag atcgttacgc tcaaactgcg tggtcgcagg
     3181 cagcacgata tcggcaaagc ggcaggttga ggtccactgg ttatctatgg cgataaccgt
     3241 ttccagtttg cgccagcctt caataatgcg gttgatctgc tgatggcgat ggaaggggtt
     3301 agttccggca aaaatacaca ttttcagcgg cggcagtttt accgatttac cgttccagtt
     3361 gattactttc cccggttcga ggatcgcatc gataaaacgg gcaatcggaa tggtgctgct
     3421 gtagcctttg taatcactgt tgtcgtgaac aggcggaatc gacgtagagc cggagaaacc
     3481 actcagaata acgcctttac gccccggcgt gcctgcgccg ttatagtgcc agccaaaacc
     3541 aaagccacca cctggcaggc caatttgccc cagcatcgcc gccagaacca caatcatcca
     3601 cgcccactgt tcaccgtgct gcatacgctg tacgcaccag ccagcaataa tctgcgttct
     3661 gttcgccgcc atctgccgcg ccagtccacg aatggtttcg gcatcaatgc cggtcagttt
     3721 ttcagcccat gcggcatctt tcggctgacc gtctttctca cccagcagat acggcaggaa
     3781 ctgctcaaaa cccacacagt agttagcgag gaagtttttg tcgtacaggt tttcactgta
     3841 caacgtatgc gccagcgcca gttgcagcgg cacgtcagtt tgcgggttaa ccgcaatgtg
     3901 cttcacatgc tcgcgcccca gatactcatg ggtggatgta acaaccggat cgatgctaat
     3961 gacctcaatt tcaccggcgg cgactttcgc tttcaactgc gcgtaatatt cataaacatc
     4021 gtgatccggg caccaccagt tcgcttgctg gtttttcagc aaatcggagc cccacagcac
     4081 aatggttttg ctgttctgca ataccagcgg ccaggaggtt tgctgttcat acacttccat
     4141 cgaaccgact acgcgcggca ggatcacctg cgcagcacca gtagagtaat ctccgcccgt
     4201 accaacgcta ttaccatgca aggcaatagc tttcgccagc atccccgaag cgttatggaa
     4261 catccccgtc gattgccaac cactggcggt cagcaaggca ctcggcccgt gagttttctg
     4321 tacgcgttcc agttcttcat agaacatgtc gagggcttca tcccagctca cacgcacaaa
     4381 acggttatca ccgcgctggg aggtgtcgct cagatggcgc ttacgcagcc agtccacgcg
     4441 taccatcgga taacgaatac gcgccgcgtt gtgtacatga tccggcaatc cggcaatcat
     4501 tttcgacgga tatttatcca gttcgaacgg ttttgccgcc acaaagcgac catccttcac
     4561 cgtcgcgcgg atagcccccc agtgcgaccc ggtaagaatg ccctctttcg agatgacagc
     4621 ctcagtcgcc gcttgcgccg cagtcgcacg gcgcgacgtt aacaatgacg gccccagcat
     4681 cccggcgacg gttaagccgc cgagttgtgc cagaaaacgc cgacgtgatg cctgaaagag
     4741 atcgttattg ttcattattt ttcttccttc ttatcgccgt gagccttacc tgcggtgtca
     4801 gacgcattca tttgcagata tttcaacaag gtgcgttctt cacgtttatc gaggctggta
     4861 aagccgatca tgccgttgag cgtgccaatc cagccgttag cgtcaaagtg agagatttcc
     4921 ggtgcgccgt ggcactggtt acaggtgccg ttgtacaacg aatccgcata agcccagatc
     4981 ggtttgatat cgttcaccat gtcacctttc ttcatccacg cggtggcctg caacttgctc
     5041 cactcggtat tggtgtcggc aacggtggtt ttctccagcg tttttacctg ctgctgcaca
     5101 tcaccacgaa tcgaggcaac aaagatgcgt ttacctggga attgggtgag tacacgctga
     5161 cgtccggcgc tttctgtcca gccggtaatt tcaatttgca gccagtcgcc gtcacgttta
     5221 aggactttca cttccgaggc aggcagcaga gaaccagagg cttctttatc gcctttcgcc
     5281 gcataaattg gcttaatatc aatagagtac agcgtgtcac cactgtcatt agcaccggcg
     5341 cgcagctcat cgaactgctt acggaagccg ctactcatat ccggtaactg gtgggcaata
     5401 cctttatgac agtcgatgca ggattgatta tctttcgctg ccaccttcat ctgacgtgcc
     5461 gcttcaggat gctgcttcgc atgatccatc gcatcgtagt tatggcagga gcgacaggtt
     5521 gccgagttgt tttctttcat tcgcgcccat tcacgctcgg caagttccgc gcgtttggct
     5581 tcaaattttt caggtgtatc aatggagtgg gcaataaagg tctggtagat atcgttgctc
     5641 gcttccagtt tgcgcttcac catgcctgga atatccggcg ggatatgaca gtcatggcat
     5701 tcagctcgca cgccggaggc gttctggaaa tgcaccgact gtttatattc ttcatacact
     5761 ggttgcatac tgtggcaact gacacaaaat tcggttgtgc tggtgacttt gatcccaacg
     5821 tgtggcaata caatcagcgc aatgccaatc acaatcccaa tagcgaccag cgccagtacc
     5881 gaccaacgag cactgggtcg gcgtagcgcg ttccagagtt tccgcataat agcccctgta
     5941 aaattatggt ttagtgaagc gatcttaatg agtaaatatg aacagcggca ctggtcagga
     6001 tgaacggctt acggcagaat atgaacagat atgaacagaa tgagtaaaac cctctgatgc
     6061 cacatcacat tgttattgtt gaagatgagc cggttaccca ggcgcgatta caatcctact
     6121 tcactcagga ggggtatacc gtttccgtta cggcgagcgg tgccgggcta cgggaaatta
     6181 tgcagaatca gccggtggat ttaattctgc tggatatcaa cttgcccgat gaaaatggcc
     6241 tgatgttaac ccgcgccctg cgagaacgct caacggtggg gattattctg gttaccggac
     6301 gcagcgatcg gattgaccgt attgttgggc tggaaatggg cgcagacgat tacgtcacca
     6361 aaccgctgga actgcgcgaa ctggtagtac gggtaaaaaa tctgctctgg cgaatcgacc
     6421 tcgcgcgaca agctcaaccg tacactcagg acaactgcta tcgctttgcg ggttattgcc
     6481 tgaatgtgtc gcgccatacg ctggagcggg atggtgagcc gattaaactg acccgcgcag
     6541 agtatgaaat gttggtggca tttgtgacca atccgggcga aattctcagc cgtgaacgtc
     6601 tgctgcgtat gctctctgcg cgtcgggtgg aaaaccctga cctgcgcacc gtcgatgtgt
     6661 taattcgtcg tttacgccat aaactcagcg cggatttact ggtgacgcaa catggcgaag
     6721 gttatttctt agccgctgat gtgtgctgat aaaaatagac cggacgaaat cccccaggcg
     6781 acagcgagcg gcggatatgt tcgcggtcgg catttttcgg cgtcagaact aaaatcggtg
     6841 ggctgacatt atcagacacc gattgcccct gtaattgcct gatggcctgc tcaactgcca
     6901 gttccccctg ccagaccatt tgatcgctgg cagccataat cactcttccc cgcttcagcc
     6961 cgcgatacac ctgatgtgaa agataaaacg acaccacggt aagcggcgtt ttcaggttac
     7021 gcccttcccc cattgccgcc tctgccgcaa tggccgttcc ggcaacgacg tcgatttctg
     7081 gatggcgttc cagcatctcc tgcaacaggt tacgctggat ttcaatatcg ttatcaccaa
     7141 gcgcaatatc aacaatacgc accgggcttc cggcaatggc tgcgcgaaaa ccctcgacca
     7201 tctccttact gcccccggcg ttatcgggtc cgggcatcaa cagcacattc agtggtttac
     7261 cgtgcgccca ttgcaccaga tatcgcccag gttgatagcc catctgaaac cagggcacac
     7321 caacgcggct tttcacctgg ggagcatcaa tagcatttac cagctcgatc accggcagat
     7381 ttgctacttg cttttgcaga tcgggaaatg acgtcgtgct actaccgagc aaaatggcct
     7441 ctgcgcccca ctgtttacac tggtcgattt gtgcttgctg ggtagccaac tggctgtagc
     7501 cgcctgcctc cagcactttt aaatccacac cgtagcggcg agcagcctcc tgcataccat
     7561 agttcaacga taaccaatat gaatctttca ggctgggata aagcgcgcac agtttccata
     7621 cgcgtttggc ttttagcggc atagaggctt gcaccgtgaa atgctgcgca tcatgccagc
     7681 gcaacaggtt atcagccgaa aatgccggca acatgaaaag ggaaagaagt aaaaatagca
     7741 gcacgcgcat gatagcctca tcaataataa ggctttatgc tagatgcatt ccgctttgcg
     7801 actcaacatt tttcacctta agtgcaccga ccgtgaattt aaccctgacc cgaagactct
     7861 ggatgggctt tgccctgatg gcgctgttaa ccctgaccag taccctggtg ggatggtaca
     7921 acctgcgctt tatcagccag gtggaaaaag acaatactca ggcattgatt cctaccatga
     7981 atatggcgcg ccagttgagc gaagccagcg cctgggaact tttcgccgcg cagaacctga
     8041 ccagtgccga taacgaaaag atgtggcagg cgcaggggcg aatgctcacc gcacaaagcc
     8101 tgaagattaa tgcgttgctg caagcgttac gggaacaagg ttttgatacc accgctattg
     8161 aacaacagga gcaggagatc tcccgttcgt tacgtcagca aggggaactg gtggggcggc
     8221 gtctgcaact gcgccagcaa caacagcgac tcagtcagca gatagtcgct gccgccgatg
     8281 agatcgcacg cctggcgcaa ggtcaggcga ataatgcggc aacctccgcc ggagcgaccc
     8341 aggccgggat ttacgatttg atcgaacaag atcagcgtca ggctgctgaa agtgcactcg
     8401 atcggctgat tgatatcgat cttgagtatg ttaaccagat gaatgaactg cgccttagcg
     8461 ctctgcgggt gcagcaaatg gtgatgaatc tggggctgga gcagatccag aaaaatgcgc
     8521 caacgctgga aaagcagctc aataatgcgg tgaaaattct gcaacgtcgg caaatacgca
     8581 ttgaagatcc gggagttcgt gctcaggtcg caacaacgtt aactaccgtt agccaatata
     8641 gcgatttgct ggcgctgttc cagcaggaca gtgaaatcag caatcaccta caaactctcg
     8701 cacaaaataa catcgcccag ttcgcgcagt ttagcagcga agtcagtcag ctggtcgaca
     8761 ccattgagct gcgtaatcag cacggactgg cgcatctgga aaaagccagt gcacgcgggc
     8821 aatacagcct gttattgctg gggatggttt cactttgcgc actgattctg atcctctggc
     8881 gcgtggttta tcgctcagtc acgcgtccac ttgccgaaca aacgcaggcg ctgcaacggc
     8941 tgctggacgg tgatattgac tcccctttcc cggaaaccgc tggcgtacgg gagctggaca
     9001 ccatcgggcg gctgatggat gcgtttcgca gcagtgttca tgcactgaat cgccaccgcg
     9061 aacagctggc ggcgcaggtc aaagcgcgta cagctgaatt gcaggaactg gtgatagaac
     9121 accgacaagc acgggcggaa gcagaaaaag ccagccaggc aaaatcggcg tttctggcgg
     9181 cgatgagcca tgagatccgc acaccgctgt acggtattct cggcaccgca cagctactgg
     9241 cagataaccc cgcacttaac gcccagcgtg atgatttgcg ggcaattacc gactccggcg
     9301 aatcgttgct gaccatcctc aacgatattc tcgattattc cgctatcgaa gcaggtggca
     9361 agaatgtttc ggtcagcgat gaaccctttg aaccgcgacc gctgctggaa agtaccctgc
     9421 aattaatgag tggacgggtg aaaggtcgcc cgattcgcct ggcaacggca attgccgacg
     9481 atgtaccgac tgcgttaatg ggcgatccgc ggcgtattcg tcaggtcatt accaacctgt
     9541 tgagcaacgc cctgcgtttt actgacgaag ggcagatcgt tttacgtagc cgtactgatg
     9601 gtgagcaatg gctggtcgaa gtggaagaca gcggctgcgg tattgatccc gcgaaactgg
     9661 cagaaatctt ccagccattt attcaggtaa gtggcaaacg cggcggcacc gggctggggc
     9721 tgactatcag tagccgtctg gcccaggcga tgggcggcga actgagcgcc accagcacgc
     9781 cggaggttgg aagctgtttt tgtttacgct tgccgttacg tgttgccacg gcacccgtgc
     9841 caaaaacagt caatcaggcg gtgcgtcttg acggtttacg tttgctgtta attgaagata
     9901 acccgctaac ccagcgaatt accgttgaga tgctgaacac cagtggtgcg caggttgttg
     9961 ctattggcaa tgccgcgcag gctttagaga cactgcaaaa tagcgaaccg tttgctgccg
    10021 cactggtgga ttttgatctg ccggatgtca acggcattac gcttgcccga caactggcac
    10081 gacaatatcc gtcgctggtt ttgattggct ttagcgccca tgtcattgac gaaacgctgc
    10141 gccagcgtac cagttcacta tttcgcggga ttatccctaa accggtgccg cgtgaagtgc
    10201 tcggtcagtt actggcgcac tatctccaac tgcaagccaa taacgatcta ccactggatg
    10261 tatcgcaact caatgaagac gctcagttaa tggggacgga gaagatccac gaatggctgg
    10321 cattatttac acaacatgcc ctgccgcttc tcgatgaaat cgacattgcc cgcgccactc
    10381 aggatagcga gaaaataaag cgtgccgcgc atcagctaaa aagcagttgc tcgagtctgg
    10441 gaatgcgtag cgccagccag ctctgcgcac aactggagca gcagccatta tctgcccccc
    10501 ttccacacga agaaattaca cgcagtgttg ccgctctgga agcatggttg aataagaaag
    10561 acctgaacgc gatttgaatc aatatcagcc agttaagaat aactagaata gtggcatctg
    10621 gtatgcaact taacggactg gcaaaatcat ggcagagaaa aaaagaaccc gctggcagcg
    10681 gcggccaggc acgacgggcg gcaaattacc gtggaatgac tggcacaatg ccacgacctg
    10741 gcgtaaagcg acgcaattat tgctgttggc aatgaatatt tatattgcca tcacgttctg
    10801 gtattgggtg cgctattacg aaacggcagg tagtacgata tttgtcgcca gaccgggagg
    10861 tatcgaaggc tggctaccga ttgccggtct gatgaatctg aaatatagcc ttgcaacagg
    10921 tcagttaccg tccgtccacg ccgccgcgat gctgttattg gtcgctttta tcgtcatcag
    10981 tctattactc aaaaaggcct tttgctcatg gttatgcccg gttggtacgc tttctgaatt
    11041 aatcggcgct ctcggtaaca aactgtttgg tcggcaatgt gtccttcccc gctggctgga
    11101 tattcctctg cgcggtgtaa agtatttgct gttgagtttt tttctctata tcgcgttatt
    11161 gatgcctgct caggcgattc actattttat gttgtcgccc tacagcgtgg tgatggacgt
    11221 taaaatgctc gatttctttc gtcatatggg gaccgcgaca ttaatcagcg tgaccgtttt
    11281 gctgattgcc agcctgttta ttcgccatgc ctggtgtcgt tatctttgcc catatggcgc
    11341 gctgatgggc gtggtttcgc tattatcgcc gtttaagatt cgtcgcaatg ccgaaagttg
    11401 tatcgattgt ggcaaatgcg caaaaaattg cccatcacgg atcccggtcg ataaattaat
    11461 tcaggtacga acagtggaat gtaccggctg tatgacttgc gtagagtcat gtccggtagc
    11521 ctcaacattg accttttcac tgcaaaaacc tgcggcaaat aaaaaagcct ttgcgttgtc
    11581 tggctggtta atgacgctac tggttctggg gattatgttt acggtgattg gttacgcaat
    11641 gtatgcagga gtatggcaaa gcccggtgcc ggaggaattg taccgacgct taattccgca
    11701 agcgccaatg attggtcact aaattagcaa caagcggaac atcacctcgc acggggtgat
    11761 gttcacaaaa tcacattatt ttgattttga catcataact ttcaagcccg gtcatttttt
    11821 ctcgcgcggt gaagcgaatt tcagagactt ctttccctgt attcttgttc agctcggcga
    11881 ttttttgcgc gataaagtcg gcgatttccg tttcggcttg ttttttaact cttcaatatt
    11941 cactgttaac ctcttctggt cttaaaagac agcaacacgc cagtaaggtg ttggttcccg
    12001 gtaccctggt caggtaagaa atgttataag ccatctcccc gaaagattca ataatcaaca
    12061 ggttatcgct tactatacat acagcgttga aaaaaaataa gacagatata aaaaaggctc
    12121 cacaagggag cctgatatta ttttgtgaaa tccagcggtg catggtcagt gcgtatttgc
    12181 agcgcgacgt tatcaaaggt gatcatcgtc gatttacaaa aaatacactt agcgcccaaa
    12241 gggtttcgct ccgtcacatc gaatgccgat gtgcggtact gtgaaccatg acaacatgga
    12301 cagcgaaaat ggagatgttg agtaataacg gcaaccttag agcgtaacta cgttcgctgc
    12361 cgcggggcca cgttgaccct gctcaataga aaattcaact ttctgatttt cgttcagcgt
    12421 gcggaattca ttgctctgga tggcggtgaa atggacgaaa acgtctttgc tgccatcatc
    12481 aggagtgata aagccaaaac ctttatctgc gttaaaccat tttactaaac cagtcatttt
    12541 attagacata ttttacttcc ttcattttgg gcctttcggc aaacagggct ttaatacaga
    12601 aactacttag tgttcaggag agagactcaa aagaagggga taatgcctga taatgagaac
    12661 tgctttagta aactactttg tatgctgtct gtctttccaa ccgacgcagc tattaacgca
    12721 tgataattga gattaagcaa attttatttt agccgtccgg cggtcttaaa tatcgctggc
    12781 tttattttat taaccccgta tagtgcagcg cagtattttc agtaaggaat ttgtttgtcc
    12841 cgtaaaatga caggaattgt caaaaccttt gatcgcaaaa gcggcaaagg attcattatc
    12901 ccctccgacg gtcgtaaaga agttcaggtc catatttccg cattcactcc ccgcgacgca
    12961 gaagtgctta taccaggatt acgcgtggaa ttttgccgcg taaatggcct gcgaggacca
    13021 acagcggcaa atgtttatct ctcttaatat gacgctgctt gtttaaagct gactggtttt
    13081 attggcatag ttcaatagtc aacaccatgt attataacac aagttacttt ttcaccggaa
    13141 aactaatcca tttaaccgaa tatctctaca actaatagtt
//
LOCUS       DBFIFS010000124        13061 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.124, whole genome shotgun sequence.
ACCESSION   DBFIFS010000124 DBFIFS010000000
VERSION     DBFIFS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.124"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            170..883
                     /gene="aphA"
                     /locus_tag="N7A07_003103"
     CDS             170..883
                     /gene="aphA"
                     /locus_tag="N7A07_003103"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU2477724.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            994..1410
                     /locus_tag="N7A07_003104"
     CDS             994..1410
                     /locus_tag="N7A07_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU2477725.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1414..1770
                     /locus_tag="N7A07_003105"
     CDS             1414..1770
                     /locus_tag="N7A07_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU2477726.1"
                     /translation="MTISELLQYCMAKPGAEQSEHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1805..4627)
                     /gene="uvrA"
                     /locus_tag="N7A07_003106"
     CDS             complement(1805..4627)
                     /gene="uvrA"
                     /locus_tag="N7A07_003106"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU2477727.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4882..5418
                     /gene="ssb1"
                     /locus_tag="N7A07_003107"
     CDS             4882..5418
                     /gene="ssb1"
                     /locus_tag="N7A07_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU2477728.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5517..5798)
                     /locus_tag="N7A07_003108"
     CDS             complement(5517..5798)
                     /locus_tag="N7A07_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU2477729.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6227..7813
                     /gene="pdeC"
                     /locus_tag="N7A07_003109"
     CDS             6227..7813
                     /gene="pdeC"
                     /locus_tag="N7A07_003109"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU2477730.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7816..8139)
                     /gene="soxS"
                     /locus_tag="N7A07_003110"
     CDS             complement(7816..8139)
                     /gene="soxS"
                     /locus_tag="N7A07_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU2477731.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8225..8689
                     /gene="soxR"
                     /locus_tag="N7A07_003111"
     CDS             8225..8689
                     /gene="soxR"
                     /locus_tag="N7A07_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU2477732.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9235..10584
                     /gene="ghxP"
                     /locus_tag="N7A07_003112"
                     /gene_synonym="yjcD"
     CDS             9235..10584
                     /gene="ghxP"
                     /locus_tag="N7A07_003112"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU2477733.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10735..12384
                     /locus_tag="N7A07_003113"
     CDS             10735..12384
                     /locus_tag="N7A07_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU2477734.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12538..>13061)
                     /gene="espX5"
                     /locus_tag="N7A07_003114"
     CDS             complement(12538..>13061)
                     /gene="espX5"
                     /locus_tag="N7A07_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HGU2477735.1"
                     /translation="NLYKVIAPYINLFRADLSFSKLDLINFKHADLSRVNLNKAILQN
                     INLIDSKLFFTRLTNTFLEMVICTDSNMANVNFNNANLNNCHFNCSVLTKAWMFNTRL
                     YRVNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNTHEV
                     AMKITADIMQHAD"
BASE COUNT         3050 a         3199 c         3429 g         3383 t
ORIGIN      
        1 aaaaaacgac aggaacgtat ttactaggtt aaatataatc atcctgcttt tcatcacaaa
       61 aaccgcagat aatccttcct ttcccctgta gctggcgtta tggtcagata gtttttgcaa
      121 caaatctcac aataaaaagt ttcaacatac tgactattta gggaaaaata tgcgcaagat
      181 cacacaggca atcagtgccg tttgcttatt gttcgctcta aacagttccg ctgttgccct
      241 ggcctcatct ccttcaccgc ttaaccctgg gactaacgtt gccaggcttg ctgaacaggc
      301 accaattcat tgggtttcgg tcgcacaaat tgaaaatagc ctcgcagggc gtccgccaat
      361 ggcggtgggg tttgatatcg atgacacggt acttttttcc agtccgggct tctggcgcgg
      421 caaaaaaaac ttctcgccag aaagcgaaga ttatctgaaa aaccctgtat tctgggaaaa
      481 aatgaacaac ggctgggatg aattcagcat tccaaaagag gtcgctcgcc agctgattga
      541 tatgcatgta cgccgtggtg acgcgatctt ctttgtgact ggtcgtagcc cgacgaaaac
      601 agaaacggtt tcaaaaacgc tggcggataa ttttcatatt cctgccacca gcatgaatcc
      661 ggtgatcttt gcgggcgata aaccagggca aaatacaaaa tcgcaatggc tgcaggataa
      721 aaatatccga attttttatg gcgattccga taacgatatt accgccgcac gcgatgtcgg
      781 cgctcgtggt atccgcattc tgcgtgcctc caattctacc tacaaaccct tgccacaagc
      841 gggtgcgttt ggtgaagagg tgatcgtcaa ttcagaatac tgacagagcg ggagagcgtg
      901 acactctccc gaatgctgtt tttttaatca cacctttatc ctttcgctgt cttgctgcaa
      961 actgattaag agagttttat caaggagcag cacatgtggt atcaaaagac gctcacgctt
     1021 agcgccaaat ctcgtgggtt tcatctggta acggatgaaa ttctgaatca gctggctgat
     1081 atgccgcgcg ttaacatcgg cttactgcat ctgttgctgc aacatacctc cgcctctctg
     1141 acacttaatg agaactgcga tcccaccgta cgccacgaca tggagcgttt tttcctccgc
     1201 accgttcccg acaacggaaa ttatgagcat gactatgagg gagcagacga tatgccttct
     1261 catatcaaat cctcaatgct gggaacatcg cttgtattgc cggtgcataa agggcgtatt
     1321 cagaccggca cctggcaagg catttggctg ggggaacatc gcatccacgg cggatcgcgt
     1381 cgcatcatcg cgacactaca aggggagtaa aaaatgacca tttcggagtt gctacaatat
     1441 tgcatggcaa aaccaggcgc agaacagagc gagcataacg actggaaagc gacgcagatc
     1501 aaagtggaag atgtactgtt tgcgatggtg aaagaagtag aaaatcgccc agctgtttcg
     1561 ctgaaaacca gcccggagct ggcggagctg ctacgtcagc agcacagcga tgtgcgtcca
     1621 agccgccatc tgaataaagc gcactggagc accgtgtatc tcgacggttc gctgccggat
     1681 tcgcaaatct attatctggt ggatgcgtct tatcagcagg cggtgaattt actgccggaa
     1741 gaaaaacgta aattgctggt gcaactctga aaggaaaagg ccgctcggaa ggcggcctta
     1801 acgattacag catcggcttg aggaagcgtg ccgtatgcga agcttcgcac tccgcgacgg
     1861 tttctggcgt accggagacg aggatctcgc cgccgccact gccgccttcc ggccccaggt
     1921 cgacaatcca gtcagcggtt ttaatcacgt cgagattgtg ctcaatcacc acaatggtgt
     1981 tgccctgatc gcgcagttta tgcagtacgt cgagcagttg ctgaatatcg gcgaagtgca
     2041 gaccggtggt cggctcgtcg agaatataca gcgtctgccc ggtgccgcgt tttgacagct
     2101 cacgcgccag cttcacgcgc tgggcttcac caccagaaag tgtggttgcg gactgcccca
     2161 agcgaatgta cgtcagacca acgtccatca acgtttgcag cttacgcgcc agagctggca
     2221 ccgcatcaaa gaactcacgc gcctcttcga tggtcatatc cagcacttcg tggatggttt
     2281 tgcctttgta cttaatctcc agcgtttcac ggttatagcg tttacctttg cactggtcgc
     2341 acggcacgta aatgtccggc aggaagtgca tctccacttt gatcacgcca tcgccctgac
     2401 aggcctcgca gcgtccgcca cggacgttaa agctgaaacg tcctggcgta taaccacgcg
     2461 cacgggattc cggtacgccc gcaaacagtt cgcgcacagg cgtaaacacg ccggtatagg
     2521 tcgccgggtt agaacgcgga gtacgaccaa ttgggctttg gtcgatatcg attactttgt
     2581 cgaaatgctc cagtccctga atatcgcgat acggtgccgg ttcggcgatg gtcgcaccat
     2641 tcaactggcg ttgggcaatc gggaacagtg tgtcgttaat cagcgtcgat ttaccggaac
     2701 ctgaaacccc tgtgatgcag gtaaacagac cgactggcag cgttagcgtc acgtctttca
     2761 ggttgttgcc gcgtgcgcca gtcagcttaa gtactttttc cggattcgcc ggaacgcgtt
     2821 tcttcggcac ttcaatcttg cgtttaccgc tcatgtactg tccggtcaac gactccggca
     2881 ccgccataat cgcttccagc ggaccttccg cgaccacttc accgccatgt acacctgcac
     2941 caggaccgat atcgatcaca tgatcagcgg cgcgaatcgc gtcttcgtcg tgctccacca
     3001 caatcacggt attaccgaga tcgcgcagat ggataagcgt acccaacagg cgctcgttat
     3061 cgcgctggtg caggccgata gacggctcat ccagcacgta cataacgcca accaggcccg
     3121 caccaatctg gctcgccaga cggatacgct gggcttcacc gccggaaagc gtttctgccg
     3181 agcgggaaag cgtcaggtaa ttcaggccga cgttaacgag gaatttcaaa cgatcgccga
     3241 tctctttaag aattttttcc gcaatcttcg cccgctgacc tgcgagtttg agattgttga
     3301 agaattccat cgcatgacca atgctcatgt cggagatggc gggcagcggc gtattctcga
     3361 catacacgtg gcgcgcttcc cgacgcagac gcgtcccttc gcagctggcg cacgggcgat
     3421 tgctgatgaa cttggctaat tcttcacgta ccgcactgga ttctgtctct ttataacggc
     3481 gctccatatt gtgcagcacg ccttcgaacg gatgacgacg gatggaggta tcgccacgat
     3541 cgttcatgta tttgaattca atgttttctt tgccagaacc gtacaacacc actttatgca
     3601 cgttcgcgct caggctgccc cacggcgctt cgacgtcgaa cttatagtga tctgccagcg
     3661 atttcagcat ctggaaataa tagaagttgc ggcgatccca gccacggatc gcaccacctg
     3721 ccagcgacag ttccggattc tggatcacgc ggtcaggatc gaaatattgc tgtacgccaa
     3781 ggccgtcgca ggtcgggcag gcacctgccg ggttgttaaa cgaaaacagt cgcggctcca
     3841 gttcacgcat actgtagccg caaattgggc aggcgaagtt ggcggaaaac agcagctctt
     3901 ccgctttcgg gtcgtccata tccgccacta ccgcggtacc accggaaagc tccagcgcgg
     3961 tttcaaacga ctcggcaaga cgttgggtaa gatcgtcacg caccttgaag cgatcaacca
     4021 ccacttcaat agtgtgtttc ttttgcagtt ccagtttcgg cggatcggaa agatcgcaga
     4081 cttcgccatc aatacgagca cggatgtaac cctggcttgc caggttctcc agcgttttgg
     4141 tgtgttcgcc tttgcgctct ttaatgattg gcgcgagcag catcagacgc ttgccttccg
     4201 gctgcgacag cacgttatcc accatctggc tgacggtttg cgccgccagc gggacgtcgt
     4261 ggtccggaca gcgcggttcg ccgacgcggg cgtacaacaa acgcaaatag tcgtggattt
     4321 cggtgattgt ccccaccgta gaacgcgggt tatgagacgt cgatttctgc tcaattgaga
     4381 tggcaggaga aagcccctca atatgatcga cgtccggctt ttccatcagt gacagaaact
     4441 gccgcgcgta ggcggaaagg gattcaacgt aacggcgctg cccttcggca tataaggtgt
     4501 cgaaagcgag cgaggatttg ccagaacccg aaagcccggt cacgacaatg agcttgtcgc
     4561 gggggataac gaggttgatg tttttgagat tatgggtgcg ggcgccccga acttcgatct
     4621 tatccattca cctttcccgg attaaacgct tttttgcccg gtggcatggt gctaccggcg
     4681 atcacaaacg gttaattatg acacaaattg acctgaatga atatacagta ttggaatgca
     4741 ttacccggag tgttgtgtaa caatgtctgg ccaggtttgt ttcccggaac cgaggtcaca
     4801 acatagtaaa agcgctattg gtaatggtac aatcgcgcgt ttacacttat tcagaacgat
     4861 tttttttcag gagacacgaa catggccagc agaggcgtaa acaaggttat tctcgttggt
     4921 aatctgggtc aggacccgga agtacgctac atgccaaatg gtggcgcagt tgccaacatt
     4981 acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa agagcagact
     5041 gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggccagcga atatctgcgt
     5101 aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac cgatcaatcc
     5161 ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat gcagatgctg
     5221 ggtggtcgtc agggtggtgg cgctcctgca ggtggcaata tcggtggtgg tcagccgcag
     5281 ggtggttggg gtcagcctca gcagccgcag ggtggcaatc agttcagcgg cggcgcgcag
     5341 tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat ggactttgat
     5401 gacgatattc cgttctgatt tgtcattaaa acaataggtt atattgtttt aaggtggatg
     5461 attaaagcat ctgccagcca taaaaaagaa gcctccgtta tggaggcttc tacgtatcag
     5521 gtcaaaatca ttggccactg tggcggtgtc tgattcatct cttctatcat caccgactcg
     5581 atgttgtgcc agatggcggt gatgtccgcc attgtgatgc caagcaaccc cattgcgaac
     5641 cagcaggcgg aaaccacgcc cagaccgctg ctgatcactg caagcccgac ataatcagac
     5701 cgacgaaaac ggatgttgag cgtgctggcc agaaacatca ttacggcact aagaagttgc
     5761 cagcgaagaa gaaccacgcc agtggtgagg gtagccatca aacaattcct ctgaaaagag
     5821 ccgatgccct ggacagcgcg gcgttgttca cgggaggcgt ggtacactct ggctatcgcg
     5881 gggcttgcag aacacaaaaa tgaaacactc tgtttgtttc attaattttg tgaactatat
     5941 cacaattgat tgtttgttag ccagattagg tcgtgacttt tattgctgta cagattatgt
     6001 ggtttttcag tggcattaag ggtatatctt cccgccgcct ctgtattcct gtagaaaatt
     6061 aattttgaat atcaatgaat tattttcatc caagtgacga ttaaaaaggt atcaatttca
     6121 aatcaggcaa aagtgctatt tataccgtaa gatttatcta aagacgtcgg tagccagggt
     6181 ttcaccttgc aatggccggg tataaacagg caggaaattg atagcaatga atcatcgtgc
     6241 acgacaccaa ttactggcgt tgccgggcat tatcttttta gttctctttc ccatcattct
     6301 ttcgctatgg attgccttcc tttgggcaaa atcagaagtg aataatcagc tccgaacctt
     6361 tgctcaactg gcgttggata aatccgagct ggtcattcgc caggcagatt tagtgagcga
     6421 tgcagctgaa cgctatcagg ggcaagtttg cactccagcc catcaaaagc gaatgttgaa
     6481 tattattcgt ggctatcttt atattaatga attgatctat gcccgtgata atcatttttt
     6541 atgctcatcg ctgatagtgc ctgtaaacgg ctatacgatt gtatcggccg attataagcg
     6601 tgaacctaat gtttctatct attattaccg cgatacgcct tttttctctg gctataaaat
     6661 gacctatatg cagcggggaa attatgtggc ggttatcaac cctctcttct ggagtgaagt
     6721 gatgtctgat gacccgacat tgcaatgggg tgtgtatgat acggtgacga aaaccttttt
     6781 ctcgttaagc aaagaggcct cagcagcaac gttttctccg ctgattcatt tgaaggattt
     6841 aaccgtacca agaaatggct atttatatgc gacagtttat tcgacaaaac gcccaattgc
     6901 agccattgtt gcgacttcat atcaacgtct tatagcccat ttttataatc atcttatttt
     6961 tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc tacgtattcg
     7021 acaaaactat ttgtctccca aacgcaaatt gcaacgcgcc ctcgaaaaac atcagctatg
     7081 cctttattac cagccaataa ttgatatcaa aacagaaaaa tgtatcggcg ctgaagcgtt
     7141 gttacgttgg cctggtgagc aggggcaagt aatgaatccg gcagagttta ttcctctggc
     7201 agaaaaggag gggatgatag aacaggtaac tgattatgtt attgataatg tcttccgcga
     7261 tctgggcgcg taccttgcaa cacatgctga tcgctatgtt tctattaatc tgtcggcctc
     7321 cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc aatacgcggt
     7381 gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg tcgacaaaat
     7441 gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg atgattttgg
     7501 tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt tgaaaatcga
     7561 caaatcgttt gttgagacgc tgaccaccca taaaaccagt catttgattg ctgaacatat
     7621 catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg aaactgagga
     7681 gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt tctttgcgaa
     7741 ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc gggagttaac
     7801 gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat caaactgccg
     7861 acggaaaacg cgggagaagg tctgctgaga gacataaccc aggtccattg cgatatcaaa
     7921 aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacagacggc gttgacgaat
     7981 gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatc cgttgcaggt accactttga
     8041 atagcctgat ttttttgcga cgacatcaat gttaagcggc tggtcaatat gctcgtcaat
     8101 ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc ttttcagtgt
     8161 tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg aggtaaagcg
     8221 atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg aagtggcgaa
     8281 acgcagcggt gtggcggtat cggcgctgca tttctatgaa agcaaagggt tgattaccag
     8341 tatccgtaac agcggcaatc agcggcgtta taaacgtgat gtgttgcgat atgtcgcaat
     8401 tatcaaaatt gctcagcgta ttggcattcc tctcgcgacc attggtgaag cgtttggcgt
     8461 gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt cccaatggcg
     8521 agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac tggacggatg
     8581 tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg gcgaccgctt
     8641 aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa gcgccacaag
     8701 ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctggt acacaggagg
     8761 gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg gttggtgttg
     8821 atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac tttttagccg
     8881 caatattttt gtcatctcag acgatttttt atcgcaatcc tcaacggtat acggctcgat
     8941 aacgctgcaa tcttgcgcat cgacgataac gtttgcgcat caattgcctg gtttttcatc
     9001 gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg ctatgatcgc
     9061 cggataatcg tttgcttttt ttaccacccg ttttgtatgc gcggagctaa acgtttgctt
     9121 ttttgcgacg cagcaaattg tcgcaaacct ggagcaggaa gataacgttt cgctggcagg
     9181 ggattgtccg ccacgcatct tgacgaaaat taaactctca ggggatgttc tcttatgtct
     9241 acgccatcag cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc acaacgtgga
     9301 agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat ggtctactcg
     9361 gttatcgtcg ttccaggcat gttgggtaaa gcgggcttcc cgcctgcggc agttttcgtt
     9421 gcgacctgtc tggttgccgg actcggttct atcgtgatgg gtctgtgggc taatctgccg
     9481 ttggcgattg gttgcgccat ctccctgacg gcgtttaccg cattcagcct ggtgctgggg
     9541 caacatatta gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt gctgtttacg
     9601 gtaatttctg ccacgggtat ccgtagctgg attttgcgca acttgccgca cggtgtggcg
     9661 cacggcacgg ggatcggtat cggtctgttc ctgctgctca ttgccgctaa cggtgtcggt
     9721 ctggtgatta aaaacccgct tgatggtctg cccgttgcgc tgggtgattt cgcgaccttc
     9781 ccggtgatta tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa actgaaagtc
     9841 cctggtggca ttctgctgac cattatcggt atctcaattg tcggtttgat cttcgatcct
     9901 aacgtccatt tctccggcgt tttcgccatg ccttcattga gcgatgaaaa cggcaattca
     9961 ctgattggca gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc aagcgttctg
    10021 gcgctggtga tgacggcagt atttgatgcc accggaacta tccgcgctgt cgccggccag
    10081 gcgaacctgc tggataaaga tgggcagatc atcgacggcg ggaaagcact gaccactgac
    10141 tccatgagca gcgttttctc tggcctggtg ggtgcagctc cggcagcggt atacatcgag
    10201 tctgcggcgg gtacggcggc gggcggtaaa accggtttga cggctatcac cgttggcgtg
    10261 ctgttcctgc tgattctgtt cctctctccg ctttcttacc tcgttccggg gtatgcaacg
    10321 gctccggcgc tgatgtacgt tggcctgctg atgctgagca acgtggcgaa aatcgacttt
    10381 gctgattttg ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt gctgacctgt
    10441 aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg tctggtttcc
    10501 ggtgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct ggtgacattc
    10561 tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt ggcaatggct
    10621 gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga aaaaatgaga
    10681 attcaggcat aacgtctttc tcagatcata aaaaacatcg caaacaggga acgcatggaa
    10741 atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt ggtcactcgt
    10801 gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc gctactggcg
    10861 tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt cttgtttatc
    10921 ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct cgaacatggt
    10981 cgagagattt tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg cattggcttc
    11041 cttatttact gggtggtgcc gggcattccg ctgatccccg cctttgcgct ggcggcggtg
    11101 ctttctccga ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg catcccgaaa
    11161 aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcctccgg cctggtgtcg
    11221 ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct tcaccgtcgg cggtgcgacg
    11281 gtcgaattta tgaaagtagc cattggcggt attctcgccg gttttgtggt gagctggctg
    11341 tacggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc gacgcagatc
    11401 gtcctgctgt tcctgctgcc attcgcttct tatctgattg ccgaacatat tggcgtttcc
    11461 ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt gatgcgccgt
    11521 gcgccgctgg caatgcgcct gcgtgcaaac agcacctggg cgatgctgga atttgtcttt
    11581 aacggcatgg tgttcctgct gttaggtctg cagctgccgg gtattctgga gacgtcgctg
    11641 atggcagcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga tattattctg
    11701 atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa aaagttcagc
    11761 aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg agaaatcctg
    11821 atcgcgtctt tcgccggggt gcgtggggcg atcactttgg ccggtgtgct ctctatcccg
    11881 ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt tctggcggct
    11941 ggtgtcattc tcttctcgct gtttgtcggc gtggtgatgt tgcctattct gctacaacac
    12001 attgaagtcg cggaccattc gcaacaattg aaagaagaac gtattgcgcg agcggcaacg
    12061 gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc ggataccgaa
    12121 gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg taacctgcgt
    12181 cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa ccttgagcgt
    12241 cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct gcgcgccacg
    12301 cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt gcttgaagcg
    12361 ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga ggtaatctga
    12421 aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc atgattctaa
    12481 aacaaataag ttatctctct tttaaagtca tttcattggc tgttcttcga cttaatctca
    12541 atctgcgtgt tgcataatat ctgcagtaat cttcatagct acttcatgag tattattctc
    12601 agtttgtgac atgccagtat gagaggtaca atcttcttca aagaatttct gtagtgttac
    12661 cagggtatca ctattaatgg gaatattctc ctccccacgg agaatggaaa tgcccattcc
    12721 ctggacgcta gcctcatcga aattaacccg atagagacgg gtattaaaca tccaggcttt
    12781 tgttaaaaca gaacagttga aatggcaatt gtttaaattg gcattattaa aattaacatt
    12841 agccatatta gaatcggtgc atataaccat ttcgaggaac gtatttgtta gccgtgtaaa
    12901 aaagagttta ctatcaatta agtttatatt ctggaggatt gctttgttca gattgacgcg
    12961 agacagatca gcatgtttaa agttaattaa atcaagttta gagaagctaa ggtcggctct
    13021 aaataaatta atatatggcg ctattacttt ataaagattt a
//
LOCUS       DBFIFS010000125        12972 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.125, whole genome shotgun sequence.
ACCESSION   DBFIFS010000125 DBFIFS010000000
VERSION     DBFIFS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.125"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            18..1400
                     /gene="ydgI"
                     /locus_tag="N7A07_003115"
     CDS             18..1400
                     /gene="ydgI"
                     /locus_tag="N7A07_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2477736.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            1437..2159
                     /gene="folM"
                     /locus_tag="N7A07_003116"
     CDS             1437..2159
                     /gene="folM"
                     /locus_tag="N7A07_003116"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU2477737.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(2156..2491)
                     /gene="ydgC"
                     /locus_tag="N7A07_003117"
     CDS             complement(2156..2491)
                     /gene="ydgC"
                     /locus_tag="N7A07_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU2477738.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            2620..3339
                     /gene="rstA"
                     /locus_tag="N7A07_003118"
     CDS             2620..3339
                     /gene="rstA"
                     /locus_tag="N7A07_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU2477739.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            3343..4644
                     /gene="rstB"
                     /locus_tag="N7A07_003119"
     CDS             3343..4644
                     /gene="rstB"
                     /locus_tag="N7A07_003119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU2477740.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            4720..5649
                     /gene="tus"
                     /locus_tag="N7A07_003120"
     CDS             4720..5649
                     /gene="tus"
                     /locus_tag="N7A07_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU2477741.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(5646..7049)
                     /gene="fumC"
                     /locus_tag="N7A07_003121"
     CDS             complement(5646..7049)
                     /gene="fumC"
                     /locus_tag="N7A07_003121"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU2477742.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(7192..8838)
                     /gene="fumB"
                     /locus_tag="N7A07_003122"
     CDS             complement(7192..8838)
                     /gene="fumB"
                     /locus_tag="N7A07_003122"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2477743.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9037..10212
                     /gene="manA"
                     /locus_tag="N7A07_003123"
     CDS             9037..10212
                     /gene="manA"
                     /locus_tag="N7A07_003123"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU2477744.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            10313..11821
                     /gene="ydgA"
                     /locus_tag="N7A07_003124"
     CDS             10313..11821
                     /gene="ydgA"
                     /locus_tag="N7A07_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU2477745.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLVKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(11866..12972)
                     /gene="uidC"
                     /locus_tag="N7A07_003125"
                     /pseudo
     CDS             complement(11866..12972)
                     /gene="uidC"
                     /locus_tag="N7A07_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
BASE COUNT         3179 a         3319 c         3169 g         3305 t
ORIGIN      
        1 ttaatcactg gatatcgatg gaaaagaaac tgggactgag cgcactcacc gcgctggtat
       61 taagctcaat gctgggcgcg ggtgttttca gtctgccgca aaatatggcg gcagttgcca
      121 gcccggcagc actgctcatc ggctggggta ttactggcgc tggcatttta ttgctggcct
      181 ttgccatgct gatcctcacg cgcattcgtc ccgaacttga cggcggtatc tttacctatg
      241 cccgcgaagg gtttggcgag ctaatcggct tttgttccgc atggggatac tggctgtgcg
      301 cagtcatcgc caacgtctcc tatctggtga tcgttttttc cgcgttaagc ttttttacgg
      361 acacgccgga attgcgcctg tttggcgatg gtaatacctg gcaatcgata gtcggtgcat
      421 cggctttatt gtggattgtt cactttttga tcctgcgcgg ggtgcaaacc gctgccagca
      481 ttaacctggt ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt gtgctggcga
      541 tgatgatgtt caaactggat atcttcaagc tcgacttcac cggacttgca cttggcgtac
      601 ctgtttggga acaggtgaaa aacaccatgc tgatcaccct gtgggttttc attggtgtgg
      661 aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc aaagcgacac
      721 tgctggcggt tctctccgct ctgggcgttt acttgctggt aacgctgctt tcgctgggag
      781 tggtggcacg tcctgaactg gctgaaattc gtaacccgtc aatggccggt ctgatggtgg
      841 aaatgatggg accgtggggc gaaatcatca tcgctgccgg tttgatcgtt tccgtttgcg
      901 gcgcgtactt aagctggacc atcatggcgg cagaagttcc gttcctggct gccactcata
      961 aagcattccc acgcattttt gcgcgccaga acgcacaagc tgcgccatcc gcctcactgt
     1021 ggttgacaaa catctgcgtg caaatttgtc tggtactcat ctggctgacc ggttccgatt
     1081 acaacacgtt gctgaccatc gcctcagaaa tgattctggt gccctatttc ctggtcggcg
     1141 cgttcttgct gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc ggtgcctgca
     1201 tttatggctt atggttattg tacgcctctg gtccaatgca cttgctgtta tccgttgttc
     1261 tgtatgcacc ggggctgctg gttttcctgt atgcgcgcaa aacgcataca catgataatg
     1321 tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt aattgcctca gttccggcga
     1381 cctggatgct ggtgggataa cgttcatccc atcgttggat agcaaaggag ataacgatgg
     1441 gtaaaaccca gcccttgcca atattaatta ctggcggagg tcgtcgcatc ggcctcgccc
     1501 tcgcatggca tttcattaat caaaagcaac cggtgattgt cagctatcgg acacactatc
     1561 cagccattga tggactgatt aatgcaggtg cgcagtgtat tcaggctgat ttttcgacca
     1621 acgacggtgt gatggcgttt gccgatgaag tactaaaaag cacccatggt ctgcgtgcta
     1681 ttttgcataa cgccagtgcg tggatggcgg aaaaaccggg tgcgccactg gccgacgtac
     1741 tggcttgcat gatgcagatc cacgttaata ccccatacct gctcaaccat gcgctggaaa
     1801 gattactgcg tgggcacgga cacgccgcca gcgatatcat tcactttacc gattatgtgg
     1861 tggagcgcgg tagcgacaaa catattgcgt atgctgcaag caaagcggca ctggataata
     1921 tgacccgctc gtttgcccgc aagctggcac cggaagtgaa agtgaattct attgcgccat
     1981 cgctgatcct gtttaatgaa catgatgatg ccgaatatcg acaacaggcg ctgaataaat
     2041 cattgatgaa aaccgcgcct ggcgagaaag aagtgatcga cctggtcgat tacttactta
     2101 ccagttgctt tgtcaccgga cgcagtttcc cacttgatgg cggtcgtcat ctgcgttaat
     2161 gcagctttat ccagcatata atcaacaccc atgcgcttat cccccaacac gcaacagaac
     2221 caacaaaagc ggcgggcaag cgcatcattc cggtgaaata ccacagcgac accaggtaaa
     2281 caaaataggg aataatcgac cacatactaa aaatgatggt tgcgcgtaag gcttcaatgc
     2341 cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa ggtcgggaaa agtggaatca
     2401 gcccggcgat ataataattt ttcgtttttg ctaaaacacc aatcaacagc actaccagcg
     2461 caccgagcgc ggctttgatt accagcccca tctttttacc ttaacacttc cataacaagt
     2521 catcagtaga atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc
     2581 gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg
     2641 gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatacag
     2701 gttaccgtag agccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat
     2761 ttggtgttac tcgacatcat gctaccaggc aaggacggca tgaccatttg tcgtgattta
     2821 cgcgcaaagt ggtccggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac
     2881 atcctggcac tggaaatggg tgcctgcgac tatattctca aaacgacgcc ccctgctgtt
     2941 ttgttagcgc gtttacgttt gcatttgcgt cagaatgagc aagccacgct gaccaaaggt
     3001 cttcaggaaa cgtcactgac tccctacaaa gccctgcatt tcggcacgtt gaccatcgat
     3061 cctatcaacc gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc
     3121 gaattattgt gggaattagc tacccatgcc gggcaaatca tggaccgcga tgcattgctg
     3181 aaaaatttac gcggcgtcag ttatgacggc ctggatcgta gcgtggacgt ggctatttcg
     3241 cggttaagaa aaaaactgct cgataacgcc gctgaacctt atcgcattaa aactgtgcgt
     3301 aacaaaggct atctttttgc gcctcatgca tgggaataag cgatgaaaaa actgtttatc
     3361 cagttttacc tgttattgtt tgtctgcttc cttgtgatgt ctctgctggt tgggttggtg
     3421 tacaaattta ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat gaacagttcg
     3481 ctgtatctga tgcgcagcga attgcgtgag atccccccac atgactgggg taagacgctg
     3541 aaagagatgg atttaaatct ctctttcgat ctgcgagtcg agccactgag taaataccat
     3601 cttgatgata tttccatgca ccggctgcgt ggcggcgaaa ttgtcgccct ggacgatcag
     3661 tacacttttt tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg tcctgttcct
     3721 tatctttatt atctccatca gatgcgattg ctggatatcg ccctgatcgc ttttattgct
     3781 atttccctcg cctttccggt gtttatctgg atgcgtccgc actggcagga tatgttaaaa
     3841 ctggaagcag cggcgcaacg atttggcgat gggcatctca atgaacgtat ccactttgat
     3901 gagggttcga gctttgaacg acttggtatc gcatttaacc agatggcgga caatatcaac
     3961 gccttgattg ccagcaaaaa acagcttatt gacggtatcg ctcacgaact gcgaacaccg
     4021 ttagtgcgcc tgcgttatcg actggagatg agcgataacc tgagcgccgc cgaatcccag
     4081 gcgttgaatc gtgatatcag tcaacttgaa gctttaattg aagagctgct gacttatgcc
     4141 cgactcgatc gcccacaaaa cgagcttcat cttagcgaac cagacctgcc gttgtggctg
     4201 tcaacgcatc tggcagatat tcaggcagta acgcccgata aaacggtacg gataaaaacg
     4261 ctcatgcaag gccattatgc ggcgttggat atgcgcttaa tggagcgcgt gctggataat
     4321 ttgctcaata atgccctgcg ctactgccat tcaacggttg aaaccagcct gctactgtcg
     4381 gggaatagag cgacattaat tgttgaggat gatggcccag ggattgcccc agaaaaccgc
     4441 gaacatatct ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc aaccggcggc
     4501 tgcgggctgg ggctggcaat tgtccactct atagcactgg caatgggcgg tacggttaat
     4561 tgtgacacca gcgaactggg tggtgcccgc ttctcgttta gctggccgtt atggcataac
     4621 atcccgcaat ttacctctgc ttgacactac gcgcacggtg gtcaagtcac caccactgtg
     4681 ctataaatta aggatgttgt aactaaagtg gttaatatta tggcgcgtta cgatctcgta
     4741 gaccgactca acactacttt tcgccagatg gaacaagagc tggctgcatt tgccgctcat
     4801 cttgagcaac acaagctatt ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag
     4861 gatgagcata atccgcttaa tcgtattgag gtgaaacaac atctcggcaa cgacgcgcag
     4921 tcgcaggcgt tgcgtcattt ccgtcattta tttattcaac aacagtccga aaatcgcagc
     4981 agcaaagcag ctgtccgtct gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa
     5041 gcagcgttgg tcagtcatat tcagcacatc aataaactca agaccacgtt cgagcatatc
     5101 gtcacggttg aatcagaact ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg
     5161 ccggggctga tcacccttaa tgcttaccgc acgctcaccg ttctgcacga tcccgccact
     5221 ttacgctttg gttgggctaa taaacatatc attaagaatt tgcatcgtga tgaagtcctg
     5281 gcacagctgg aaaaaagcct gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag
     5341 tggcaaagaa aactggagcg agagtatcag gatatcgctg ccctgccaca gaatgcgaag
     5401 ttaaaaatca aacgtccggt gaaggtgcag ccgattgccc gcgtctggta caaaggagat
     5461 caaaaacaag tccaacacgc ctgccctaca ccgctgattg cactgattaa tcgggataat
     5521 ggtgcgggcg tgccggacgt tggtgagttg ttaaattacg atgccgacaa tgtgcagcac
     5581 cgttataaac ctcaggcgca gccgcttcgt ttgatcattc cacggctgca cctgtatgtt
     5641 gcagattaac gcccggcttt catactgccg accatctgtt ctggccgtac ccagctgtca
     5701 aactcggctt cgctaagata ccccagcgca agggccgcag cttttaaggt cagcccttct
     5761 ttatgcgctt ttttggcgat ctccgcggct ttgtcataac caatgtgggt gttaagcgca
     5821 gtcaccagca tcagcgattc attgagtaat tgattgattc gctcacgatt cggttcaata
     5881 cccactgcgc agtgtttgtt aaaactttcc atgccatctg ccagcaagcg caccgattgc
     5941 aggaaattgt ggatcaccat tggacggaag acgttcagtt caaagttacc ggaagcgccc
     6001 cccatgttga tcgccacgtc gttccccatc acctgacagc agagcatggt taatgcctca
     6061 cactgtgttg ggttcacttt ccccggcatg attgagctgc ccggctcatt ttccgggatt
     6121 gagatttcac caattccgca gcgcgggcca gaggccagcc agcggacatc attggcgatt
     6181 ttcatcagtg acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag
     6241 gtcgccagcg cttcaaattt gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc
     6301 agttcatctg ctacgcgacg cgcatactcc ggatgggtat ttagtccagt acccaccgct
     6361 gtaccgccaa gagccagttc cgctacgtga ggcaggctgt attcgatatg tttgagatta
     6421 tgctcgagca tcgctaccca gccggaaatc tcctgcccca gtgttagcgg ggtggcgtcc
     6481 tgcaagtggg ttcgaccgat ttttacgata tcggcaaatg cacgcgattt ttcactcagc
     6541 gtctgtgtca gggttttaag ctgcggaatg agttgcttgc gcagcgccag cagcgccgca
     6601 acgtgcatcg ccgtcggaaa gacatcgttg gaactttggc ttttgttcac gtcgtcgtta
     6661 gggtgaactt tacgttccat cccgcgcacg ccgccgagta attcactggc ccggttagcc
     6721 agcacttcat tcatgttcat gttactttgc gtgccggagc cggtctgcca gatagccagc
     6781 gggaattcgt cgtcatgctg tcctgccagt acttcatccg ccgcctgccg aatggcgctc
     6841 gctttctctt cagacaacaa gcctaaatct tcattaactt ttgccgctgc gcgcttggtt
     6901 agcgccagcg catgaatcag tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc
     6961 agcgagcgtt gagtttgtgc gccccacagc ttatctgccg ggacatcaat cgcccccatc
     7021 gaatcttttt cgctgcgtac tgtattcatg gcctactcct cacctgatta attttttctt
     7081 tctgttttgc tttcgttaag taacttttag ctcactcatt atttactatt tgataacaaa
     7141 tgtttggtct ttcgtgccat gtaaaaaaac cgccccgaag ggcggctctg tttatttcac
     7201 acagcgggtg cattgtgtga gttgtatctg ctggaagaag tcatttcctt tatcatccac
     7261 aaggataaac gccgggaaat cttccacttc aattttccag atggcttcca ttcccagttc
     7321 cggatattca acacattcca ggctcttaat acttccctgc gccaatacag cggccggacc
     7381 accgatactg ccaaggtaga agccaccgtg ttttttacag gcatccgtca cctgctggct
     7441 gcggttgcct ttcgccagca tgatcatact tccgcccagc gcttgcagtt gatcgacata
     7501 agaatccatc cgtccggcgg tcgttgggcc aagagaaccg gaggcataac cttccggcgt
     7561 tttggccgga cccgcgtagt aaatcggatg atctttgatg tactgcggca gcccttcacc
     7621 gttatccatc cgctctttca gtttggcgtg agcaatatca cgaccgacga taatcgtgcc
     7681 gttaagcgat aagcgtgtag aaacgggata ctgcgacaac tgtgcgagga tctctttcat
     7741 cggacggtta aggtcaacgc gcaccgcttc gccttctccc gctttgcgca gctcttccgg
     7801 gatatatttg cctggattat gttccagttt ttcgatccag atcccctgac ggttgatctt
     7861 cgctttgata ttacggtcag cagaacagga gacgcccata ccgaccgggc aggatgcgcc
     7921 gtgacgtggc aggcgaatca cgcggatgtc gtgagcgaag tatttaccgc caaactgcgc
     7981 acccagacca agattttgcg cttcgatcag caattctttt tccaattcca catcgcggaa
     8041 cgcctgaccg tgctcattcc cttccgttgg cagttcatca tagtatttcg cggaagccag
     8101 tttcaccgtt ttaaggttcg tttctgcaga agttccacca ataacgaacg caatatgata
     8161 cggaggacag gccgccgtac ccagcgtgcg catcttctca accaggtaat ttttcagttt
     8221 ccccggcgtc agtaacgctt tggtttcctg atagagatac gtcttgtttg ccgaaccacc
     8281 acctttggcg atacagagga atttgtattc gtcgccatca acggcataaa gatcgatctg
     8341 cgctggcaga ttggtgccgg tattcacttc tttatacata tccagcggcg cgttttgcga
     8401 gtagcgcaga ttatcttcga tataagtgtt atagacaccg cgcgccagcg ccgcttcatc
     8461 accaccaccg gtccatacac gctgcccttt tttaccaaca ataatcgcgg tgccggtatc
     8521 ctgacaggtt ggcagaacgc ctttcgccgc gatgtcggag ttacgcagga attgcagcgc
     8581 cacatattta tcattttcgc tggcctccgg gtcacgcaga atgtcggcca cctgttgttg
     8641 gtgcgccgga cgcagcatga acgacgcatc atgaaacgcc tggcgcgcca acagagttaa
     8701 cgcttcgggt gcgactttca aaatctcctg cccttcaaat tcagataagc taacgtgttc
     8761 gctggttagc aggtaatact cagtatcatc ttttttgagt ggaaaaggag cctgataatg
     8821 aaagggtttg tttgacattg ttttctcact tactgcctgg tttggttatg ctctgggcgg
     8881 gtgttccgtt gccctgttaa aagcgagtaa caatatccta cgcacttttt taacaaaaac
     8941 tgagactagt acgacttttt gcggctccag gttacttccc gtaggattct tgctttaata
     9001 gcgggattaa tttccacatt aaaacaggga ttgatcatgc aaaaactcat taactcagtg
     9061 caaaactatg cctggggcag caaaacggcg ttgactgaac tttatggtat ggaaaatccg
     9121 tccagccagc cgatggccga gctgtggatg ggcgcacatc cgaaaagcag ttcacgagtg
     9181 cagaatgccg ccggagatat cgtttcactg cgtgatgtga ttgagagtga taaatcgact
     9241 ctgctcggag aggccgttgc caaacgcttt ggcgaactgc ctttcctgtt caaagtatta
     9301 tgcgcagcac agccactctc cattcaggtt catccaaaca aacacaagtc tgaaatcggt
     9361 tttgccaaag aaaatgccgc aggtatcccg atggatgccg ccgagcgtaa ctataaagat
     9421 cctaaccaca agccggagct ggtttttgcg ctgacgcctt tccttgcgat gaacgcgttt
     9481 cgtgaatttt ccgagattgt ctccctactc cagccggtcg caggtgcaca tccggcgatt
     9541 gctcactttt tacaacagcc tgatgccgaa cgtttaagcg aactgttcgc cagcctgttg
     9601 aatatgcagg gtgaagaaaa atcccgcgcg ctggcgattt taaaatcggc gctcgacagt
     9661 cagcagggtg aaccgtggca aacgattcgt ttaatttctg aattttaccc ggatgacagc
     9721 ggcctgttct ctccgctgtt gctgaatgtg gtgaaattaa atcctggcga agcgatgttc
     9781 ctgttcgctg aaacaccgca cgcttacctg caaggcgtgg cgctggaagt gatggcaaac
     9841 tccgataacg tgctgcgtgc gggtctgacg cctaaataca ttgatattcc ggaactggtt
     9901 gccaatgtga agttcgaagc caaaccggct aaccagttgt tgacccagcc ggtgaaacaa
     9961 ggtgcagaac tggacttccc gattcctgtt gatgattttg ccttctcgct gcatgacctt
    10021 agtgataaag aaaccaccat tagccagcag agtgccgcca ttttgttctg cgtcgaaggc
    10081 gatgcaacgt tgtggaaagg ttctcagcag ttacagctta aaccgggtga atcagcgttt
    10141 attgccgcca acgaatcacc ggtgactgtc aaaggccacg gccgtttagc acgggtttac
    10201 aacaagctgt aagagcttac tgaaaaaatt aacatctctt gctaagctta ttaaaggctt
    10261 ataacacctt caggcggcca gtccgcctga tttcatttta tggataatca ttatgaataa
    10321 atcgctggta gcggtaggcg tcattgttgc gctaggcgta gtctggacag gcggcgcatg
    10381 gtatacaggc aagaagattg aaacccatct cgaagacatg gtcgcgcagg cgaacgcgca
    10441 actcaaactg accgctcctg aatccaacct ggaagtgagt tatcaaaact atcatcgcgg
    10501 cgtattcagc agtcagctgc aactgttggt gaaacccatt gccggaaaag aaaatccgtg
    10561 gattaaaagc ggtcagagcg tcatcttcaa cgaatcggtt gatcatggtc ccttccccct
    10621 tgcccagctt aaaaaactga acctgatccc gtcgatggca tcaattcaaa ccacgctggt
    10681 taataacgaa gtaagcaaac cactgtttga tatggcaaaa ggtgaaacgc cttttgagat
    10741 taactcgcgc attggttaca gcggtgattc cagttccgat atttcgctca agccactaaa
    10801 ttacgagcaa aaggatgaaa aagtcgcctt tagcggcggc gagttccagt taaatgcgga
    10861 cagagacggc aaagctatct ccctttccgg ggaggcgcaa agtggtcgga tagacgcggt
    10921 taacgaatac aaccagaaag tacagttgac ctttaataat ctgaaaaccg acggttccag
    10981 cacgctggca agttttggtg agcgcgtagg aaaccaaaaa ctgtcactgg taaaaatgac
    11041 catttcagtg gaaggcaaag aactggcact gctggaaggc atggagatca gcggtaaatc
    11101 ggatctggtc aatgacggta aaacgatcaa tagccaactg gattactcgc taaacagcct
    11161 gaaggtacag aatcaggatc tgggcagcgg caagctgact ttaaaagtcg gccaaattga
    11221 tggcgaagcc tggcatcagt ttagccagca atataacgcg caaactcagg cgctgctggc
    11281 acagccagaa attgccaaca atcccgaact ttatcaggag aaagtgacgg aagccttctt
    11341 tagcgccctg ccgctgatgt tgaaaggcga tccggtgatt actatcgcgc cgctaagctg
    11401 gaaaaacagt cagggtgaaa gtgcgctgaa tctgtcgctg ttcctgaaag atccggcaac
    11461 gactaaagaa gcgccgcaaa cgctggcgca ggaagtagat cgttcggtta aatctctgga
    11521 tgcgaaactg accattccgg tggatatggc aactgagttg atgactcagg tagcgaagct
    11581 ggaaggttat caggaagatc aagcgaaaaa actggcgaaa cagcaagttg aaggtgcatc
    11641 agcaatgggg cagatgttcc gtctgaccac cttgcaggac aataccatca ccaccagcct
    11701 gcaatatact aacggtcaga taacgttaaa cgggcagaaa atgccactgg aagatttcgt
    11761 tggtatgttt gcaatgccgg cattaaatgt tccggtcgta cccgctattc cgcagcagta
    11821 attcacagcc aaaacgccgg agatttttct ctccggcgtt attttttact tcagcataaa
    11881 gtcatactga atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc
    11941 atcgcgcttt tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc
    12001 ttcgctgacc cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg
    12061 tttactggat tcatagccgc gagtcacaat cggtgcaaat gtcagtgtaa attgggagtt
    12121 aaacgatagt tgacgcccaa ttgccaggac tgcattcctt cacggttacg atcaagactt
    12181 aatgaaaagg gatagccaat gtcggtatcc accacggcac cattcattgc atggcgcaaa
    12241 taaccaccac tgccaaatac gcttccccac ggcgcattcc aggtcaattg tccactaacc
    12301 aacgccgttt cattgggctg gctggtatta cgacttaaac cttcgagttg cgcgtaaaag
    12361 ccgagcaact cccctttcaa cgtataattc gggtcgagcg gtatgctttt ctcgagaata
    12421 ctttgtatct gattggaaac atctttctgt accgatgccc ccagagaata gcgactggta
    12481 tcatcatgcc aactggcggc taaaaaagaa cgagcgcgat gtttatcgta ttttcccgtc
    12541 tggctgttat acaccccttc atcacgaata ccggttgttg aatcagtcca taaaaatact
    12601 cgtgaacgcc acattcccca cagatcaata tttggcgtta aagcccaata acctaatgct
    12661 ccttcagaaa ctgtcggtaa tgtacgttgc gaactgccag ccattaacgg aatacgataa
    12721 ggtaagctac cataactata atccgtcccg aagcgaccta atttaagcag gctattttca
    12781 ctgggtttta tttttactgc cccgagggca aagccaaaac ttttatcacc atcaagatac
    12841 caccgggtac tcatatcagc acgagcacct aatttataaa cataatacgc tccgccttca
    12901 acgccaataa tattggaata ataaccacta ttgaaatcga gcaatcctcc ctgtacccat
    12961 gcgtaaatat ct
//
LOCUS       DBFIFS010000126        12925 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.126, whole genome shotgun sequence.
ACCESSION   DBFIFS010000126 DBFIFS010000000
VERSION     DBFIFS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.126"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            141..224
                     /gene="idlP"
                     /locus_tag="N7A07_003126"
     CDS             141..224
                     /gene="idlP"
                     /locus_tag="N7A07_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU2477746.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            221..613
                     /gene="iraD"
                     /locus_tag="N7A07_003127"
     CDS             221..613
                     /gene="iraD"
                     /locus_tag="N7A07_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU2477747.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(606..1460)
                     /gene="hypT"
                     /locus_tag="N7A07_003128"
                     /gene_synonym="qseD"
     CDS             complement(606..1460)
                     /gene="hypT"
                     /locus_tag="N7A07_003128"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU2477748.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(1582..2754)
                     /gene="iadA"
                     /locus_tag="N7A07_003129"
     CDS             complement(1582..2754)
                     /gene="iadA"
                     /locus_tag="N7A07_003129"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU2477749.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(2767..3228)
                     /gene="yjiG"
                     /locus_tag="N7A07_003130"
     CDS             complement(2767..3228)
                     /gene="yjiG"
                     /locus_tag="N7A07_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU2477750.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(3225..3908)
                     /gene="yjiH"
                     /locus_tag="N7A07_003131"
     CDS             complement(3225..3908)
                     /gene="yjiH"
                     /locus_tag="N7A07_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU2477751.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            4158..4708
                     /gene="kptA"
                     /locus_tag="N7A07_003132"
                     /pseudo
     CDS             4158..4708
                     /gene="kptA"
                     /locus_tag="N7A07_003132"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            complement(4865..10107)
                     /gene="iatD"
                     /locus_tag="N7A07_003133"
                     /pseudo
     CDS             complement(4865..10107)
                     /gene="iatD"
                     /locus_tag="N7A07_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(10318..11496)
                     /gene="yjiJ"
                     /locus_tag="N7A07_003134"
     CDS             complement(10318..11496)
                     /gene="yjiJ"
                     /locus_tag="N7A07_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2477752.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            complement(11564..12406)
                     /locus_tag="N7A07_003135"
     CDS             complement(11564..12406)
                     /locus_tag="N7A07_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU2477753.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            complement(12672..>12925)
                     /locus_tag="N7A07_003136"
     CDS             complement(12672..>12925)
                     /locus_tag="N7A07_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2477754.1"
                     /translation="QANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQAKN
                     STEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
BASE COUNT         3179 a         3149 c         3242 g         3355 t
ORIGIN      
        1 tttgatgatt tttatcaaat catgactttt ttcctgaaaa gtcagtagat attcataggc
       61 aagtaaggtt ttatactttg ctgacaggat tcaggcctgt ctcagactga catggatgta
      121 atgaacaaaa gggaatggct atggaaaatg agcatcaata cagtggtgcc cggtgttcag
      181 ggcaagccgc atatgttgct aaacgtcagg agtgcgcaaa atgatgcgcc aatcacttca
      241 ggctgtttta cctgaaattt caggcaataa aacgtcattg ctgcgtaaat cggtatgcag
      301 cgatctcctc actcttttta attctcctca ttcgacatta ccgtcattac ttgtttcagg
      361 catgcccgag tggcaagtgc ataacccgag cgacaaacat ctccagagct ggtattgccg
      421 tcagctgcgt tctgcactgt tatttcatga gccacggatt gcggcgttgc aggttaattt
      481 gaaagaagcg tactgccaca ctctggcgat cagccttgag ataatgctgt accacgacga
      541 tgaaccgcta acgtttgatc tggtctggga taatggcggt tggcgcagtg cgacgcttga
      601 gaatgtcagc taagcacaat ctccagctcg cgcagttcac gccagaagcg ttcggcaact
      661 ggattcatgc gggtattcat ccggtatgcg taggcctgaa tcgggatcac cagttcgtcc
      721 cgattcagca caacgagctg cccgctgcga atttcttgtt ggatggcata ctccggcagc
      781 caggcaatcc cacagccgtc gagggcaacc tgctttaaaa gctcgctcat cgaagagaca
      841 aaaaaggtgc tgaaacttaa ctcactgtgg cgcgtcaggg tgcgattaat caatcgcccc
      901 atgtaggagt tgcggctgta attcagtaac ggaaagtgtg gctgcacgag gtcaaaaagt
      961 gcttctccat gttcgtcact ggcgcagaca gggaacaatt gagattcaaa taagcgaatg
     1021 tggtcaaacg gcgcttccag caaatcttcg tcgtgaaagg aaaaaataca gtcactttgt
     1081 ccttcacgca gtttatcgac cgcttcatcg acatcaatgg cttcaatcgc ccaggtaaag
     1141 agcggcggca tctggctgat aatggacggt aacagaccga gggaaagaga gtgtgcagcg
     1201 gctatcttga ttttacgttg cgcgtaatcg ctgccgccac gcagctctgc aaggttgctt
     1261 tctaactgtt gcaacagatg gcggatctgc gaatggaaga ttttcccttg ttctgagagt
     1321 tgcagcggcg tcacctggcg gttaaacaat tcaacgccaa tcgcctgttc cagcgcgcgg
     1381 atgcggcggc tgaatgccgg ttgcgagacg ttacgactga ctgccgcctg ggaaaaattg
     1441 cggcattttt ccagggtcag aaaatcttaa agccatttgg tttcaatatt atgcaaaacc
     1501 gcaccacagt catccattac gttttactct gtcagccgcc cttgggggca ttctacgtcc
     1561 attagggcgg ctgacaaccg tttaagccgt ttcaaacgtt cctttcacgc aggctttgcc
     1621 gtctttgacc atcagtttgc cgcgagcgta tacctgctca atgcgcaatt ctggcgtcat
     1681 gaccagtaag tctgcgtcat tgcctggcag aatttcgcct ttcccggtca ggttaaggaa
     1741 accggctacg ctactggtga gcgggcgcag ggcatcgctg atactgaaat catagtcttt
     1801 aaccagcacc tgcacggttt ccagcaacgt ttcaaaacct gcaacgccga tatgggttaa
     1861 attcccttca tcatcgaaga acggctggct accgttgccg tcggagctga gggtgacgcg
     1921 tgccagcgga atacccgcct gaacggcgcg gacaatacct tcggcagggg cgaccggttc
     1981 gtcaatgctg ctggtgatat cgatggtgcc gcctttgcgc gcgaactcca gcgcctgctc
     2041 aaacaacggt acgttgcggt taacgtgggt cggcagcagc ttgctgatcg gcacatcgca
     2101 gttttccagc aggtcataaa taggctgtaa tgccttttta ctgtcgccca tgtggaacac
     2161 ggtgacgcca ggtttaccgc cgagcaaacc gccaacgcgg gattctgccg ccatattagc
     2221 caggtgataa acgtccggtg cggcagaacg gtgatcagag atggcacatt tcacgccaat
     2281 cacacgatcg ataatcgcca cgtctttttc cacggaaccc gtaatggtgc gggaagggac
     2341 atgataagcg ccggtcagca tccaggcgct gatgccttct tcattgagcg cacgggtctt
     2401 ggcgagcagg gattccgggt ggcgagagat agagtcggtg cccagcagac caaccactga
     2461 cgtgacgccc gcttccgtca ggcgacttag cgccacttcc ggcgtgcgcg tcgtgggacc
     2521 tgcttcgccg ccaccgccaa tcaaatggac gtgttgatca ataaaacctg ggcagaggat
     2581 ctgcccgctg agatcgacaa ccgtgcagtc cggtacaatg tcagaaggga tattactggc
     2641 aacggcgata attttgccgt tagcgacgag gacatcgcaa attccccgat cttccggcgc
     2701 atacaaatgc gctccctgca gcagggtaaa accggctgcg gtataatcaa tcatggtaac
     2761 tccttgttaa acaataagct gcataaccca gatcgacagc aatgcgttaa tcacgcagac
     2821 cgtaatgata tgcgggtaat atttggcatt cacttcggct gtccccaggc agcgaccgac
     2881 attttgcacc gggttgccca ttaaatacat cgcgggcagt aaaacggtga catcgtgacc
     2941 ggttaatgcg ccagcggttg ccagactggc ggcaacaccg actgcgccgc ccatactcat
     3001 taatgcggct aaaaggacgg ttgccgcttc accgggtaat ccccacagcg ccattacggg
     3061 ttcacagata tgccccaccc agtcgagcag gccggtaatt ttcagcgcct gaatgatgac
     3121 aaacgccatc accacgtttg gcagcaggtt ggtcgtggcg atagtaaagc cacggcgcgc
     3181 gccatcgata aacatatcca tgacgttttt gcggacctga gttgtcatgc ttgcgctcct
     3241 tgcgttgggt tgcgacgttc ttcgaagtta agccagacgc gcaaaatgtt ggctccgaca
     3301 aatttaaaca ccaggatgac ggctaaaggt acgattacgg acgtgcccag aaaggcgaac
     3361 accgcgacgc cggaagagaa gtagttggtg atgatggcgc taccgctggt ttgatatgcg
     3421 gcgaaaatca ctttatcgcg ttcggtaatt tcaccttcct gcgccagctc tttggtcata
     3481 ccggcggcgg catcagtgtt ttgcaagtta gcgatcagcg ccagcgagca gatccctgga
     3541 atgcccaaca acggttttag cacaggtgtc attaactgct gcgcggcgcg taatccacca
     3601 agaccatcgg taattgaaat aatccccaga gagagaatga ctgacggtgc cagctccagc
     3661 gcaaagagaa agccatcttt agcccccgcg ccacccgcgc cgcgaaatga ggtggtggct
     3721 ccattcgcac caggcagttg cccaaacgat ccgttcagaa cggaaaaatc aaaaacgcgc
     3781 caccagctgt ctgttccgga aaaaacaccg gaaaaaaaga ttatggtgag aaaaaaagcc
     3841 agataccctt taatgcccac tttttctgtc gccagctccc cggctacagc atccccttgt
     3901 tgtgtcataa caatccccat tgtttttttg tgtttgtgtg ttgcttgagc caggaaaatt
     3961 cctggaactt aaaaacctta tcagccatgt gggtatgggg aaaatgcaag tatgtttctg
     4021 gctatgcgtt ttttgcatag gggaaaaatt acgctgtgtg gaaaaggttc tgcgcaggat
     4081 tgagttgcag aaaattcata aacccagtga caatgacatt ggtaaataaa aacagaaaat
     4141 ccagggacga aaacgcgatg gcaaagtaca acgaaaaaga gctggcggat acgagcaagt
     4201 ttttaagttt tgtcctccga cacaaaccgg aggcgattgg tatcgtactg gaccgtgaag
     4261 gatgggcgaa tatcgataag ctcatacttt gtgcccaaaa agcgggcaag cgattaaccc
     4321 gtgcattgct ggataccgtg gtcgcaacca gcgataaaag cgttttagtt attccagtga
     4381 tggtaggtgc atccgcgcgg ttcaggggca ttcgacttcg caggtcgcta tttcctttgc
     4441 cgaaaaaaca ccgccacagt ttctctacca cggtacggca agccgtttcc ttgacgagat
     4501 aaaaaaacag gggctgattg caggggatcg tcattacgtg catctctctg ctgatgaagc
     4561 aacggcacgc aaagtgggag caagacacgg atcgccggtt gtcttaaccg tcaaagcaca
     4621 ggaaatggcg aaacgaggca tccctttctg gcaagcggaa aacggggtct ggctgacatc
     4681 aacggttgct gttgagtttc ttgagtagtc gtttatgccg gctggggtac aaaaacaata
     4741 aattcaatat attgcagggg ttatataggc ctgataagcg cagcacatcc ggcaatgtag
     4801 cgtttgttat cagtctgaaa ggataccctc gtaaaagggt atcctttaat aacaactgct
     4861 gaaattaaag tgatttacgg caggcggtca gttgggggct ggaatcagcg tcgcttccgg
     4921 tcataccggt tagcatctgc acatagtagt gccgactgta atcatcagat tcacttgtcc
     4981 agtaagctcc cggtgtaaat tgcgaattac tggaaaacgc tccaacgttc ccccattcac
     5041 catacagtga tccctgcgtg cgtttgtttt cgccagacgt caaatgtgtg atgccaggaa
     5101 gattatatcc atcattttta gcatgacaga gcgcattggc agctagccag atattggtat
     5161 gatttccatt attcacccac cagcctttca gcttaattaa ggtctggtag gttgtacctc
     5221 cttgtcgggg aacagcgctt attgtgactg acgtttgatc gccgatattt gcgaaagaaa
     5281 ctttacccga agcatcgaca gacacccaac cctgtgaact tgaaaacata taatcatcaa
     5341 cggttttacc cgcttcaaag ttatcattgt tgagtgtgta atacgctcct gcaaagcctt
     5401 cgctcgggaa tttagccgtg gagaaggtat ggttgtttgc agtgacatga ccggacattt
     5461 cttttatcga ttggatgaga ttcagcgata tgcttaagtt tgcctgatga accccgttca
     5521 gcatgggaat gagtgtcgct gttccctctt ttccgccagt gacagtcacg gtgtactcgc
     5581 cataatttct ttggctgtaa tcaatatcgc tcaacttgag aaagttcacg aaggggcctg
     5641 actggacaaa ttccagatga tcagacgtca ctatcgggtt accgtacttg tcttgcaatg
     5701 ttagcgtaag ttttgcgctc tgctggtagt cagccttcag agtgtcaaca tcggatttca
     5761 aaattgactt actttcatcg gccgtgccgg caaccaatgt aatggttgcg gattggttgg
     5821 ctgtgccagg aacttgcaca ttgactgtta aggtttctgc caatgtactg gttattgtcg
     5881 ccgtgacttg cccggactga tcggtacgcg tagttggaat attaactacc gggctgcctt
     5941 tggtgtctaa gccatgacta atatcgacac cttcaaccgg gaatccgtaa tcatcttcta
     6001 ctcgtgaagt gatggtgctg ctatcgatgc cgtcggcggt gatggtgtca ggagaagcca
     6061 cgacgctcac aacatgtgcc gttttagcat ctgccttaac cgcaaccgtt acctggctct
     6121 gaacgggaac atctttgccc attatggccg taacggtata gacattcact ttgtcactgg
     6181 tcagcgtcac tttggcctga cctgactggt cagtaaggac ttcccctgta ttgcttagcg
     6241 ttgcaccttc aggcgttacg ctaaaggtga taatttgccc tggcatcaat tcaccccgcg
     6301 catttctcgc cgttgcggtc agcgtaatac cctcactatt atggaccgta gtttctggtt
     6361 tcgatgatgt taactcgagt ttgccatttg ccacattcac gtcaacctta ctactactgt
     6421 tattgtccag cgttgccgtc acctgataaa ccccaggttg ggtgctgaga aggttaacgt
     6481 acgcataacc gttattatcg gtgtaagccg tatcgcctat cgtaatgtta gttgttggct
     6541 gcgcacttaa tctgacgcgt tgacccgcga ttacattgtt aaacgcgtcc tggacgagta
     6601 tccgtagcgt gatgttttcg cccaccgtta tcgttttttc ggctccggtt atacttatta
     6661 ccttcgccga actttcatcc ggtagcactg ataaactaaa cgttttatta tcggaagctt
     6721 gcgctttccg gctcaccacg acgttatgtt gtccggcaat attgctggtc attgttaccg
     6781 tcgcctgacc cagcgtattg gtggtgactt ctgtttcgct tagagtgaca tcgttactct
     6841 ggaaagtgac caactggtct ttaagcggat tttggtaagc gtccaggatc gttgccgtca
     6901 gggtactccc cgtactgtta cccgcaacaa tggaatttaa tgaggcagtc agcggctgga
     6961 gctgcgcact gtttgtatcc ccaataaaag tgacttgctg ataagcaacg gtattatttc
     7021 tggtcagcgt cgccgtaact gtaaactcac ccgcttttgt gcctcgcaat gtgaccacgg
     7081 catcaccgtt tgcatcggtg cgggcggttt cactacttag cgtgaagttc ccggtcatgg
     7141 aggctggcaa ggtaaatttc acatcctcat ccggcaacgg gtgtccataa acatctttca
     7201 cactggcagt cagggtaacg atatttgtat tgttagcgat cgcactgccc gacccggcat
     7261 tcaaggtcat taccgattgt tccctgtttg ggaccacggt gatcgtgacc gactggtgaa
     7321 tatttttatc cacctcaagc gaggcatcga cggaatagct accagctttt gaactggtca
     7381 ggtgcacccg cacaataccg gactggtctg ttgtggcggt cgtgttgtcc agttgcgagc
     7441 caccaggtgc cacaaaattc actttcatat cttttaacgg gttgttatga aagtctgtga
     7501 tgatggcggt cagagtactg ccctggctgt tgccagcaat aatggtatct ggatcggcaa
     7561 ccagttgttt gaattttggc gtactcgaat cgctaacgaa tttcgcaatt atccgttgtg
     7621 aaaaaccatt tggcagcgtg gcgctaatcg tattttcttc cgcaacctga cttaccatgt
     7681 ttattgtggc aacaccatat gcatccgtat tttgctgcgg cggttcgaca aactccgcgc
     7741 ttcctttttc attcttaaag gtgacctgat gatttgtcag gggattatca ctctcatcag
     7801 tgacgttgat cgtgagggta ttaaatgtct ttttatcggc gtaaacgtca ttatttgtcg
     7861 ctgataatgt ggcaacgcga gccttattct ggtttgcctc aatgttataa atatgcgatt
     7921 gcagtggagc attccagttg tgaaggctaa gttgtgccct tagtgcagtc ccttgcttat
     7981 aggccggata attcgcggca taaacccccg gctgctcttc gtgccagaca atcgtggcgc
     8041 caggtttgct gttttcgaca gtaacggctt ttaccaattc ctctttttga tatgcaaccg
     8101 gttgattgct atcgtctttc agttccaccc taaccttgat gtcgtctccg gcataatacg
     8161 atacgttatc aattgttatt gatgaatggt cgcgggatga gacaacaggg acgatattaa
     8221 cgacgatgga ttcttttacc gcactctcac cgttaatttg cggcgtcagt gttactgaac
     8281 ctgatgtcgt tccggcggtc agtatctgtg tgtaactacc atcaccgtta tctgtccaat
     8341 cagacagggt gatatcctga acgccttcac tgcgggtttg cagcgccagc cccggcaccg
     8401 gagttccgtc ggaatcgtgc gcagtaacgg tcaatgtggt cgtcgatttt ttatctgcag
     8461 ccacggttag cggattgacg gataacagag aatctttctg gcttaatgtc ggagcctgaa
     8521 taactaccat gctttgttca tgccgtgaca ggttaccttt tacatcctcg gcggtaacgt
     8581 ctatcgacca tgtattatcg gtttctgggg tgttagtgaa gcggtaacct ggcagcgtga
     8641 ccgtgatatc ttttccagac gtgctgactt tacctccggc agcttccagc gcagcggctt
     8701 cgatgttata gcctttaagg gcatatttgg tctgtagcga ggaaaccagc ggttttattt
     8761 ctccagactt ccctttcacc ggatccagca gactcaggcg aatcagctct ttcttgcggt
     8821 attccagaac gatgttgttg ttgcgatcaa tcaggtcata acgactaccg gccagactgc
     8881 gccgtccggc cacttcgtcc ggattaagct gtttctgcat tgaactgctg ggttgccagg
     8941 tcagatcaac ggcaaaacgt gtgtcatttt caccttgctt cccctgacgc tgttccgcac
     9001 tgagagtcag aagcgggaag ggggtatagt tgaggcctgc ggtaatagca tgggggttac
     9061 tttgacgatc attcttgtca aacagcgcca cttcatcgcc atagtattgt tcatagacca
     9121 gttttccccc cagttgtggc caggcaggta accagccttc cgcgcgtaaa tcccagccgt
     9181 tggccgggcg ggcttcgaag tcgttatcca attctggtgc gctacgccag ccggtcaggc
     9241 cgatataagc gttgctgctc aacttcagat aatcacgcca gtattctgcg ccaagccctg
     9301 cgcgggagtg atagcggctc aggtcgtggt caaaaaaaaa ggttgatgcc tgacatccag
     9361 ctggaggtga aatgacgcca gcccaaaccg gtgttgatct gggtacgatc gtctgttcgg
     9421 tgaagggtat gctggctgaa gagcagataa tcaggtgtgt cataccacgg atgcaggaag
     9481 tcgaattgcg aatttttcag gctaaaatct tcatccacac ccagagagat tctcgcagta
     9541 ccaaagttat ttaaccaatc agtcatcgcg cctgaggctt ctgaagacgc ccaaccacgc
     9601 gccatgccgc tggcctgctc actattcata tcttgtgaaa gcagagtgcc aactcgctgc
     9661 gaggtgctgg ctatttgatt ctccagcgtg ttttcgccat tcgcaggcgg tacggcattt
     9721 tgttgctcat ggcttttctg cgaggttgtt gccggaacat ccagttcttc accctggcgc
     9781 acgttatcaa agccgcgagc aaaagtacgg aactgattaa gacgacgaag ctcctccagt
     9841 gaaataccga agcgattagc aacgctttgc gctgattcca gcgcaccaag cgtgtaaggc
     9901 acagtattat tttctagtag aggggggggc agatgaaact gtcgtctgag gtttagcatg
     9961 taccacattt tgtgccgtcg ccatgacggg gaaaaaaaat tgtgtaaata aacaaactcc
    10021 ggcggtcagg cggcgtattt tgtcacccag accacataaa gagttttgat cattattttt
    10081 tatttgatgc gcatttgtcg aagccatagt gaccttccgt gaaaaaacag aattaaactt
    10141 gttttttcta gcacggagat ataatgcgat cctgactata atatgaccag aaataatgaa
    10201 ttagccgtgg aattgctaaa ccaggaataa taccggcggt ggctgagtac ctccaaagaa
    10261 aaaggatatt ttaattattt tacgtgatgt gctgaaaagt taaattagtt aaattactta
    10321 agaataattc gtatgcaatt tgaatatttg catcccgcag attaatgctg cgataaatag
    10381 cgccgctgcg ccgcagagtg tggcgctggc gagcgccgac gttccgttgc cgagcatact
    10441 ggtcagcgcc gggccaagaa tttgcccgat accataaatg agtgtcacaa agcccaaaag
    10501 gttaagattt cccggcacgc taagctggcg ggcgatggtc atcaccagcg aggtcgttcc
    10561 cataaaggtg ccgccaaaac caatactgct gatgatgagt aataaaggag agctgctggc
    10621 gagggttaac agcacgcaga tcgcctgcac cagcaaattc gcggtcaggc aaggtaatgc
    10681 tccccaccgt ttggctgccc acagccagcc aaagcaacct gggacaatcg acaagccaac
    10741 cagtgtccag aggtgagccg tcaacacagg ctggcccgcg tctttcgcca tgagcggcag
    10801 gtaggtggcg acgatgatat aaccaaaacc cgccagacca tacagaatcg ccagtaacca
    10861 ccagctcatg ggttgttgcg cgatttttgc caatggcgct ggcgggataa cgtgtttatt
    10921 cgacgggatg agcagcgcca gagcaagcaa tataatggca gaaagtgctc cggcaccttg
    10981 ccacaacgtt tgtgaagaga gggcaaaatg caggcctgcc agcacatatt cattacccag
    11041 agcgatgccg acgccaacac cagaaaatag cgccgcaagg acaaagggat ggcgagtatg
    11101 ttgcatgatg agtgttgagc cgaaaatcaa catcccggcg ctggcgactc ccgccagaaa
    11161 gcgaatgatg aaaaccagaa gaaacggcgg cagccacgcc atcgcgagga ttaataatcc
    11221 ggttgcgagg gcggaagcta acaggaacgg gcgcaggcgt gagggtaaat gaaacgcgcc
    11281 gaatgaaaac agcaggctcc ccgccagata cccggcatag ttaccactgg cgatccatga
    11341 gagttcgcta aatgaaaact cgccttccgc cagcatgact ggcagcatcg gcgtatagag
    11401 aaagcggcct aaacccatac ctaaggtcag aaccagcatc ccgaataacg cggtgctgaa
    11461 agaaaaacgt tctacgggat gcgatgtcga attcatgttc tgcctggttt gtattatttt
    11521 gagttaacaa gttaataaca aaaaaggaga acctgccaga ctgttatcgt gattttgtcg
    11581 acgtaaagcg ataaaaaaga ttcggttcgc caacgatata gatgttgccg ctatcatcca
    11641 tcgctatccc ttccgcctgc ttgatgttgt gtgaaaggcc atacttacct ttggttaacg
    11701 acatttcgcc gatcacgtca cctctcacgg tcacttcctg cagggcgcgt gactcatgtg
    11761 acaacaccag caacgtattt ttctgtgggt taaattccgc gccagaaaca tcgtctaaag
    11821 taaactgacg ctgcaacgtt ttatctttgc tgatatgcaa ctcatcgcta cgcaatagcc
    11881 cggttacttt gtagacctct atcggatttt tctctttaaa aaaccagaag gtatggtcct
    11941 gagaggagta agccagccct tcaaagccac agttagttgg tgtttcctgc aatgggattt
    12001 taattttttt gaggatgctc acttccgagt cagcgttaag tgaaatcaca taaatggcgt
    12061 agtcacgttc atcactgatg acaaatttat tatcaccaat gtattcaatc gtctcgagat
    12121 ccttaacaaa atcgagcgga atggtgcgga tcaagtcgcc ctcggtcgtc atttcaacga
    12181 tggttgcagg tttgttgata gtgctaaata aggtattgct ctgcgctgac caggtcagtg
    12241 aggagatatt tttagttact cctgtaatct ctttaccatc gatagtggcc tgataattct
    12301 gaaaccctgc gacatgctgg cttttacgga cgcaggattg cacaaaaaac aggcttgccg
    12361 ccacgatggc gcaaagaaaa acgataaagg tgatgcgctt tttcaactta ttgctcctta
    12421 ttattgcttt tttctcgtca aattcagtgc gacagataag gagtatacgg atgaagctta
    12481 atgaattctt aacgcgggcg atgaaatagt aaacgccgca gggggcggcg gttgtctctg
    12541 gatggcagaa cattttcagc ttacatcgac tgataaatgc gcctcatcta tgaatgatag
    12601 atgaggcggt taacgtttaa atgttaatct atatcgtctg ccatacccac tctgtttggg
    12661 tgattaatga actattcttc aactatatat gttggctgat aagacgttaa acccggaatg
    12721 gaaaatctcc tattcacttc tttctttaaa cgggcttgat taaaactgtt actgtcctgt
    12781 tgctcggtac tgttcttggc ttgtttatcg gtactgtcat tgacctgttt atcgatattg
    12841 tcattggctt gtttatcggt actgtcattg gcttgttgca ccgtactgtc aatggattgt
    12901 tgcaccgtac tgtcattggc ctgtt
//
LOCUS       DBFIFS010000127        12683 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.127, whole genome shotgun sequence.
ACCESSION   DBFIFS010000127 DBFIFS010000000
VERSION     DBFIFS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.127"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..218
                     /locus_tag="N7A07_003137"
     CDS             <1..218
                     /locus_tag="N7A07_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU2477755.1"
                     /translation="LAALLNANIELGNDPIIGIEAYPGTAEIHAKMGYKVIPGDEDAP
                     LKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(359..1132)
                     /gene="uxuR"
                     /locus_tag="N7A07_003138"
     CDS             complement(359..1132)
                     /gene="uxuR"
                     /locus_tag="N7A07_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU2477756.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1347..2807)
                     /locus_tag="N7A07_003139"
     CDS             complement(1347..2807)
                     /locus_tag="N7A07_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU2477757.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2888..4072)
                     /gene="uxuA"
                     /locus_tag="N7A07_003140"
     CDS             complement(2888..4072)
                     /gene="uxuA"
                     /locus_tag="N7A07_003140"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU2477758.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            4412..5755
                     /gene="gntP"
                     /locus_tag="N7A07_003141"
     CDS             4412..5755
                     /gene="gntP"
                     /locus_tag="N7A07_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU2477759.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5929..6831)
                     /gene="fimH"
                     /locus_tag="N7A07_003142"
     CDS             complement(5929..6831)
                     /gene="fimH"
                     /locus_tag="N7A07_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU2477760.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6851..7354)
                     /gene="fimG"
                     /locus_tag="N7A07_003143"
     CDS             complement(6851..7354)
                     /gene="fimG"
                     /locus_tag="N7A07_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU2477761.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7367..7897)
                     /gene="fimF"
                     /locus_tag="N7A07_003144"
     CDS             complement(7367..7897)
                     /gene="fimF"
                     /locus_tag="N7A07_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU2477762.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7907..10543)
                     /gene="fimD"
                     /locus_tag="N7A07_003145"
     CDS             complement(7907..10543)
                     /gene="fimD"
                     /locus_tag="N7A07_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU2477763.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10610..11335)
                     /gene="fimC"
                     /locus_tag="N7A07_003146"
     CDS             complement(10610..11335)
                     /gene="fimC"
                     /locus_tag="N7A07_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU2477764.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11372..11911)
                     /locus_tag="N7A07_003147"
     CDS             complement(11372..11911)
                     /locus_tag="N7A07_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2477765.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11976..12524)
                     /gene="fimA"
                     /locus_tag="N7A07_003148"
     CDS             complement(11976..12524)
                     /gene="fimA"
                     /locus_tag="N7A07_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2477766.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3012 a         3328 c         3106 g         3237 t
ORIGIN      
        1 cccttgccgc attacttaat gctaatattg agttgggtaa tgacccaata attggtatag
       61 aagcctatcc aggtaccgcg gagatccatg caaaaatggg atataaagtt atccccggtg
      121 atgaagatgc gccactcaaa cgcatgactc tgcaaccgtc atctcttcca gaactgtttg
      181 aattaaaaaa tggtgaatgg aattatattg gcaaataacc actgagaaga atgagtacta
      241 acattcaggt tatatagata tttaaacaat accctgcaat ttacaaagta atactcattg
      301 cgacttctta atttaagaaa gcgcaaggaa cgtttaccct tgcgcttatt ataataagtc
      361 aggcgtcgac tttatccagc ggccatgaat caaacagata gccgtcaaaa taaatatcgt
      421 caacgttcga gaattccagc agacgttgct taacgttttc cagatgctgc cacattgcca
      481 gcttcgccgc tcgggcatct tttttgatta acgcggcgag gatctgtttg tgatcgccca
      541 accactcttt gcgatacagg ctgtcatcca gatggctgtg caactggatc cacattgggt
      601 tgttttcccg ccactgccag gactgacgga acagctccac cagcatgctg ttatgcgttg
      661 cttcggcaat agcgagatgg aactgcatgt caccgctttc gctgctgccc ggcgcactgg
      721 aagccagttc acgctcttcc agttgcaatg cctgacgcat tttgacgata tcttcgcggg
      781 tagcctgcag ggcggcaaac tcggcgatgt tactttccag taactgacgc gcctgtaaca
      841 gctcaaaagg acccgcatca ttgcagacgt tggcatccgg actgtctgtg ttgtggctgc
      901 ctgagctatc aagaacatag ataccggcac cccggcgcac ttccaccagc cctttgatct
      961 ccagcatgat cagcgcttca cgtaccaccg tccgcgtgac atcaagcatt tctgcaattt
     1021 cacgctccgg cggtagccgt tcaccaggat tgtacggcgt ctttatgatc agatcgcgga
     1081 tcatcgcccc gacttcctgg taaggtcttt gcgcagaggt ggcagatttc atggtgttgt
     1141 gtccgttagg tgcggttaat caaatttttg ccggatgcgg cgtgaacgcc ttatccggcc
     1201 tacaacgggc aattttacgg cccccgttaa tctcaaacta taccattagc actggaagtt
     1261 ggcacgtaca ggtgggcaaa aaaacgaaaa ctggtcaacc aaatatcagt ttgattgacc
     1321 agtaagtctg tagggtaatc agttagttac agcgcagcca cacactcgcg cgcaccgcgt
     1381 tcgcacagct gctgatatgc cgcagtcacc gcgccaacaa agtcggcatt ctgcggcaga
     1441 tcatcggcaa aaataccgct caggcccagc agcgctttca cgcggtctgc gccctgatac
     1501 tgcgcgttaa tcttctggaa ctcggccagc atcgggtcga ccacgtcaat ggcattaccc
     1561 tgctcatcca cgccctgggt gtaacgcatc cagccagcca cgcccagcgc caggtgacgc
     1621 cagctgccgc cgttttgcag gtgcagacgc accgggtcca gcagacgctg cggtaacttc
     1681 tggctgccgt ccatcgcaat ctgccaggta cggtgacgca gagacgggtt gctgaaacgc
     1741 tcgatcagca gcgtcgcata ggcgttcagg tccgtacctt ccggcatcga cagcgttggc
     1801 gcttgttcct gcatcatcag ggcaaaggcc gctttgcgat aatccgggtt agtcatggtg
     1861 tcggcaatag tttcatagcc gccgaggtaa ccgaggtacg ccaggaaaga gtggctgccg
     1921 ttcagcatac gcagtttcat catttcgaac ggcacaacgt ctgcaacgaa ctgtgcgccc
     1981 actttatccc agtccggacg accattaacg aagttatctt caataaccca ctgacggaac
     2041 ggttcgcagg caatggcgca cgggtcgtaa acacccagct ggtcagcaat ttcctgtaag
     2101 gtttctggcg tcgccgccgg aacgatgcgg tcaaccatgg tgcacgggaa ggtgacgttc
     2161 tcttcaatcc atgccgctag ctgcgggtca cgcgcctgag ccagccccag taccgcgacc
     2221 ttcgccacat gaccgttttc acgcacgtta tcgcaggaca tcaccgtaaa tgctttcagc
     2281 cctttttcac gacgcagacg caaggcttcg acgatgtaac caatcgcgga cttcggcgca
     2341 gtcgggtttt ccagatcgtg cttgatcagc gggttattga gatccagttg accgcttgcc
     2401 gcatcagcgc agtagccttt ttccgtgacc gtcagggaga caatcgccgt ttgcggacgc
     2461 gccatcgcgt tgagaatacc ttcgcagccg tcgatttccg ggtgcagcgc ttctttcatc
     2521 gaaccgataa ttttcagctc ggtgctttcc gcgccttttt ccgccacggt gtacagcagt
     2581 tgctgttttt tcaggttttc gatcagcacg cggtcgttgc ctggcatcag gttaacttcg
     2641 cagatacccc agtcgctgtc ggtgctttcc agcagatgat gggtgtacag cgcctggtgc
     2701 gcacggtgaa acgccccgca accgagatgc acaatgcgtg attccagacg agaatgatcc
     2761 catgacgggc gggcaaccgg cagattgctg tcaacaatag tagtcatcgt aaactccaga
     2821 atccgtcttc agagagtgac ggcaaggaag aaggagccgg cacggcgcgc agccatgccg
     2881 gtgggtatta atggctaaag aaagcgcgct ggatcgccag ttcgacaccg cgaacttcgg
     2941 ccaggccttt cagacgacca attgcggagt aaccagggtt ggttttcttc ttcaggtcgt
     3001 ccagcatctg atgaccgtgg tccggacgca tcgggatcag gtcttctttg ccttccgctt
     3061 tacgacggtg ttcttcttca acaatcgctt tcaccacttc gtacatatca acgtcaccgt
     3121 tcaggtgcgc cgcttcgtgg aaggttttcg ggttatcttc acgcatggtg gagcgcagat
     3181 gggtgaagta aatacgcgga ccgaactgct tgatcatatc aaccaaatcg ttgtcagcac
     3241 gcacgccgta ggaaccggtg cacatggtga aaccgttcgc catgctgttt acggtatcaa
     3301 ccatccactg catatcttca atggtggaaa caatgcgcgg caggccgagg atcgggcgcg
     3361 gcggatcgtc cgggtgaaca gccatacgga cgccaacttc ttcggcaacc ggaataatcg
     3421 ctttcaggaa gacagcaaag ttttcgcgca gtttggcttt gtcgatatct ttgtacagct
     3481 ccaggtgctt acggaactgg tcgagggtgt acccttcttc cgcacctggc agaccagcga
     3541 tgatgttacg ggtcagacgc gctttgtctt catcgctcat agtggcgaag cgttcagctg
     3601 cctgagcaat ttcttcttcg gtgtaatccg cttccgcgcc tggacgtttc aggatatgca
     3661 tttcgaatgc agcgaattcg atctggtcga agcgcagagc tttggagccg tctggcagca
     3721 cgtattcgag gtcagtacgg gtccagtcga gcaccggcat gaagttgtag cacacggtgc
     3781 gaatgccgca ctgcgccagg ttgcgcagag tctgctgata gttagcgatc cactgctcat
     3841 agttgccagt gtgggttttg atatcttcgt gaattggtac gctttctacg acagaccaca
     3901 ccaggcctgc gtcttcaacg atcgccttgc gtttgaggat ctcttctacg gaccatactt
     3961 cgccgttcgg gatatggtgc agcgcggtaa ccacgccagt tgcgcccgcc tgacggacat
     4021 cagctaaaga aaccggatcg tttgggccgt accagcgcca ggtctgttcc atgatttttc
     4081 ctctttcaca tagtgggttc cacagcaaag agacgcggaa ccctggagtt gtttaggtat
     4141 ccggatgaac aaccagaccg cagccgtggc gttgcatcgg atgagatggc gtataagttc
     4201 taccttttgg taagacatgt caacccgaat tctgaaattg gttaaccaca tcacaagaat
     4261 ttcactctca tggttgttgc tgcaaattgc agcaaagggt aatcgatggt tattgatcag
     4321 aggatgtgac attcatcgca acaatggttg accaatttac ataacatgtc gcccaaaata
     4381 acaacggttc aaccaggtaa ggtaaaacgt tatgcatgtg cttaacattc tctgggtggt
     4441 attcggcatt ggtctgatgc tggtactgaa tttgaagttc aaaatcaatt caatggtggc
     4501 tttgttggtg gcggcgctgt ccgtcgggat gctggcgggc atggatttga tgtcgctgct
     4561 gcacaccatg aaagcgggct tcggcaacac gctgggggaa ctggctatca tcgtggtgtt
     4621 cggtgcggtc atcggtaaat tgatggtcga ctccggcgcg gctcaccaga tagcgcatac
     4681 gctgctggcg cgtctcggtc tgcgctatgt acagctgtcg gtgattatca tcggcctgat
     4741 ttttggtctg gcgatgttct atgaagtggc ctttatcatg ttagcgccgc tggttattgt
     4801 cattgccgcc gaagctaaaa ttccgttcct gaaactggcg atcccggcag tagcagctgc
     4861 cactaccgca cattcactgt tcccaccgca gccgggtccg gtggcgctgg tgaatgctta
     4921 tggcgcagat atggggatgg tttatatcta tggcgtactg gtgacgatcc caagtgtaat
     4981 ctgcgcaggt ctgatcctgc cgaagttcct cggcaatctt gagcgcccaa cgccatcatt
     5041 cctgaaagca gatcaaccgg tagatatgaa caatctgccc tctttcggcg tttcgattct
     5101 ggtgccgctg atcccggcga tcattatgat ctctaccacc atcgccaata tctggctggt
     5161 aaaagatacc cctgcctggg aagtggttaa ctttatcggt tcctcgccga ttgcgatgtt
     5221 tattgcgatg gtggttgcat tcgtactctt tggcaccgcg cgtggtcatg acatgcagtg
     5281 ggtgatgaac gcttttgaaa gcgcggtgaa gagtattgca atggtgattc tgatcatcgg
     5341 tgcgggtggc gtgctgaagc aaactatcat cgacaccggc attggcgaca ccatcggcat
     5401 gttgatgtcc cacggcaata tctcgcccta cattatggca tggctgatca ctgtgctaat
     5461 tcgtctggcg acgggtcagg gtgtcgtttc ggcgatgacc gccgccggga ttatcagtgc
     5521 tgcaatcctt gatccagcaa ctggtcagct ggttggcgtg aatccggcgc tgctggtgct
     5581 ggcgacggct gcgggttcca acaccctcac ccacattaac gatgcatctt tctggttgtt
     5641 caaaggttac tttgacctgt cggtaaaaga cacgttgaaa acctggggtc tgctggagct
     5701 ggtcaactcc gtggttgggc tgattattgt gctgattatt agcatggtag cgtaaaagaa
     5761 caccagagcc tgccaatggc aggctcagtt cggattgtcg gcaaagtgcg ctttgtttat
     5821 gccagatgcg acgctgacgc gtcttatctg gcctacaaag ggctaacgtg caggttttta
     5881 gcttcaggta atattgcgta cctgcattag caatgccctg tgatttcttt attgataaac
     5941 aaaagtcacg ccaataatcg attgcacatt ccctgcagtc acctgcccgc cggtacgtgc
     6001 gtaatttgcc gttaatccca gacttaccgc cgaagttcct actgctccta acgataccgt
     6061 gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc cctgcgctgg
     6121 tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg tgccggagag
     6181 gtaatacccc aggttttggc ttttcgcaca ataaacggta agaggaattg gcactgaacc
     6241 agggtagtcc ggcagagtaa cggtgacatc acgagcagaa acatcgcagc cgccagtagg
     6301 cactaccaca tcattattgg cgtaaatatt ccacacaaac tggaaatcat cgctgttata
     6361 gttgttggtc tgtcgcaaaa taagcacggc aattaatgag ccagctttaa tcgccacccc
     6421 gcccgcactg ctcacaggcg tcaaataaag cgccaccggc cacggcttat ccgttctcga
     6481 attataaaca acccgcggcg tttcgctggt ggtcggaaat ggatagctac tgccactata
     6541 ttttacggtc ccggaaaaat tagataacac gccgccataa gccgagcctc gttgcagtgt
     6601 gacatagtct gtaatggttt ccggataatc gttatggcaa aagatttgcg tcgaaagatc
     6661 tacgaccagg ttttgcccca cattcacggc aggcgcaagg tttacataaa cattagcgct
     6721 gccaccgcca atagggatag cggtaccatt ggcggtttta caggcgaatg accaggcatt
     6781 taccgaccag cccatcagca gtacagcaaa cagggtaata actcgtttca ttacaattat
     6841 ctcttcgggt tcagctgtag gtataggtga tgctaatcac tgcctgaatg gttccctgag
     6901 tggctccgcc atttaccgtc aatgctctga cctgtaacgg gaagtgcgct gattgtgagg
     6961 aatcatccac ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca ctgtcatcct
     7021 gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg gtactgtcgg
     7081 ctgccccgct gaagctggca gtgacccttg acgttcccac cggacaatta gtcaactcaa
     7141 gcgcaacatc atgccaggcc gatgccgccc cggcagacat cagactgaaa gaataaagat
     7201 cgccgagatc aaccgtggca ttggtggtgg aaactgtgca cggtttggcg acgaccttac
     7261 cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc gccaatattg
     7321 ccgccaataa atacccacgt ttgcgccatt tcatgagcat ctccagttac tgatattcaa
     7381 gagtgaaggt agccgtggca ttgatatgcc ccgcagtgac aggcacctgt gtcgccatta
     7441 gccgggcgta aaaattcaac gtatttggtt tacccggcgt cagggtcgtc cacgaaatcg
     7501 cggacgatgg agcattaagg ggtatctgat tttgctgctc attcagaagc tgtattccca
     7561 gtcccgaagc cgctgacacc gtattttcaa gtgcaagcag gttggcattg tggctatctg
     7621 caacgccggt aaacccaact tttacggcag aaacggcgtt accacagggt gacagcaaaa
     7681 tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt aaattgcttc gccgcgtttt
     7741 ccatcagatc aacagtaaaa ttggttgatt cagcggccac actacagccg ttatctctga
     7801 catagccgcg gatagtaatc gtgctatccg cagccaaagc gtgactcact gccagccaca
     7861 aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa cgacattcag
     7921 ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa ttggcgacac
     7981 aatgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaga ggcattccgc
     8041 tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc tctgatgtca
     8101 ccatcgcccc aaacggcagc ggcttattat tgtgggttag cgtcatgagc agttttatcc
     8161 caacgcgcgc tttaaactct gctcgcacga tcgccccacg agtgggaaca acgttagcga
     8221 ccgcgttatc taaatcgacg ttatcagcca gggtattggt atccagcgcc actctatttt
     8281 cccgatattc agtggcataa ggcagcacgg cataaccgcg ccagtcggta cgcacccccg
     8341 tctggttttc gacttttgca tcttttgcgc caggcgcttt aacaagcacc accgtatcgt
     8401 ttaacggctg ccccagcgtt acgccattgg catgagccag taccccaccg ctgactccgt
     8461 aatagagctg cttaatatca tcgctatggc tgtaaccgat attggcattg ccgtaaccac
     8521 cgcgataatt cagcgtggcg tagcctgtgc ttccgctatt accatcgcct cccccggcat
     8581 agccggtttg cacgctatag ctgaggttgt tgtcttccag caacgtaccg tatacaccag
     8641 ccagattggt catccgaccg ttgagatcgt gtgacatgct gtagctggca ctggcatgtc
     8701 gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg acgttacgcg
     8761 ctaacatctg atcacgtcct ttttgccagg cgtttttcgt caggctatag ctgagcgtcc
     8821 agttgatatc ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg acattactcg
     8881 ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgagcgc ccgagttgct
     8941 gagtaacggt gagttgtaat ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga
     9001 atttcggctt aacctgaata actccgtcct gtgtttcgat gttgtagcca ttcattcgac
     9061 tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa cccactaact
     9121 gaatattcgt gcctgactca ttgagcgatt tgttatagag aaaacgcacc gattgtccgt
     9181 catgctgact gtcatcggga agtgtggaat tagcctgagt catatccaca gacagagcgc
     9241 ccagtgcccc catatttttc ccgataccaa aattaaaagc acgataacga tctgccagtt
     9301 gcgttccacc atatattgtc cagccagctg gaaggccgtg gagtaatgta ctttggaaaa
     9361 agcggggttt ttcctgttgc gcatttccac tacggtattc tcctgccgta atggaataac
     9421 gagtatgccc ttcacgttgc aaaagcggga ctgacgaata gggtacggta aaaatctgcg
     9481 tgctgccgtc agcctcttta atcgttacct gcaagtcacc actattacct gcggcataga
     9541 tatcgttgat ggtaaaaggc cccggcggca ccgtactatt ataaatgtca tacccatttt
     9601 gtttaatagt gacctgtgca gtaccacgag caataccgtg gatcaccggg gcaaatcctc
     9661 tttggctatc gggtaacata ttgtcatctg aggccaattg tgcgccgcga aagttaatac
     9721 catcgaaaat atcaccctga gtataaccat cacccagcgt cagccgggaa cgtaacggta
     9781 ttatgtctcg ctcaagccag gtattgatat gctgccattt atttttgcta cctgatgatc
     9841 tgtcgctact gttataactc caggtggtat tgtcgcgtaa acgccacgca ccaatattta
     9901 acccactctg taggtttaaa tatgcataat ggctgttacc cccaatccga ttctgtacac
     9961 tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc cataactcag
    10021 gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc aggttcagtc
    10081 gctgctgacc aacatctaaa tgcgcagtag cgtcctggac cattgtggtt aatggcacac
    10141 aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc cccatactgg
    10201 cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc gtattaaatg
    10261 tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga tacgtccctg
    10321 gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg ggatcatccg
    10381 ctaaaaagcg cggattaaaa tagagttcgg cagatgacaa aggtgcctgt acggcaaaag
    10441 cacaggcgac aaagagccgg acaaaaaaac cagccaaacg atgcttacga atatgcaagc
    10501 attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct ccggtttaaa
    10561 atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt tattccatta
    10621 cgcccgtcat tttgggggta agtgcaccat aatcatttat tgttcggtaa gtaatattgc
    10681 ttcccgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc aaggcatttt
    10741 caagaacccg ggctccggca ttcaactctg ttaccgtcag gtaatagggt gtcgggttaa
    10801 tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg
    10861 gcaacgctaa tttagccgga cgatagtaca gtttaatgcg gctgataatt gcgagctgta
    10921 gcgtattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg ttcatccaga
    10981 ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata cgtaaggtat
    11041 tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga ccatccttta
    11101 caccatcggc attttccacc catgattgaa ttaaataggt actattttca tcattatttg
    11161 tcacggcaag ttgcacttgt ttttgccctg ccggataaat tatgcgagtc gcacctaagg
    11221 ccactcccgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg atacctgcca
    11281 gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta ctcactgtcc
    11341 tgttcctgtt atcacattat ctgctgaaca attactgata ggttaaagag aaccaggcct
    11401 gtgcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg gcgatgaaat
    11461 gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga ttaatcggta
    11521 cgaggtttcc ttcatcatca aacaacgcta cgccaatatt ggtggctatc cctggcccct
    11581 ctcccacgga aagcacatcc ggatttttac catccgcgac accgtgaaac gccacaccca
    11641 cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc accggtgcgc
    11701 tatcttcccc taccgcatga aaccggttac tgctgatttg ccccatattg accgtcattt
    11761 gtttatcacc ggcttcaatc aggcaagttt ccgcaataat gacgccctga aattgcatat
    11821 ttccgccggg cggcgtggta ttccatttat ttccggccag agcaaacatt ggcagcaacg
    11881 acgctaatag aaataatctc ttccttttca tcgttatttt tatcgcacaa gggtgggcat
    11941 ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt gatactgaac cttgaaggtc
    12001 gcatccgcat tagcagcacc cggggttgcc tcgccgattg cataataacg cgcctggaac
    12061 ggaatggtgt tagtaccgtt attcagggtt gtttgctcac tgaatgtcgc accatccagc
    12121 gtcagcgcag cacccgttct gtccaggatc tgcacaccaa cgtttgttgc gctacccgca
    12181 gctgaactct gcagagccag aacgttggta tgacccgcat caatcgccgt acctaaaaag
    12241 gcaacagcgg ctttagatgc aacattggta tcgcaatcat tcagctgaat gttaaaaccg
    12301 acagcagaac tggttgctcc gtcctgtgcc agcgatgcgg tacgaacctg tcctaactga
    12361 acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac ttccccttta
    12421 aagtgaacgg tcccaccatt aaccgtcgtg gcagcggcca gagccgctgt agaactgagg
    12481 gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct ttcctttcaa
    12541 aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg tcaacgagtt
    12601 tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc cattttgact
    12661 catagaggaa agcatcgcgc aca
//
LOCUS       DBFIFS010000128        12559 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.128, whole genome shotgun sequence.
ACCESSION   DBFIFS010000128 DBFIFS010000000
VERSION     DBFIFS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.128"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            216..1730
                     /gene="caiT"
                     /locus_tag="N7A07_003149"
     CDS             216..1730
                     /gene="caiT"
                     /locus_tag="N7A07_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU2477767.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1761..2903
                     /gene="caiA"
                     /locus_tag="N7A07_003150"
     CDS             1761..2903
                     /gene="caiA"
                     /locus_tag="N7A07_003150"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU2477768.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3032..4249
                     /gene="caiB"
                     /locus_tag="N7A07_003151"
     CDS             3032..4249
                     /gene="caiB"
                     /locus_tag="N7A07_003151"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU2477769.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4323..5876
                     /gene="caiC"
                     /locus_tag="N7A07_003152"
     CDS             4323..5876
                     /gene="caiC"
                     /locus_tag="N7A07_003152"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU2477770.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5985..6770
                     /gene="caiD"
                     /locus_tag="N7A07_003153"
     CDS             5985..6770
                     /gene="caiD"
                     /locus_tag="N7A07_003153"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU2477771.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6776..7366
                     /gene="caiE"
                     /locus_tag="N7A07_003154"
     CDS             6776..7366
                     /gene="caiE"
                     /locus_tag="N7A07_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU2477772.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7485..7880)
                     /gene="caiF"
                     /locus_tag="N7A07_003155"
     CDS             complement(7485..7880)
                     /gene="caiF"
                     /locus_tag="N7A07_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU2477773.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8141..11362)
                     /gene="carB"
                     /locus_tag="N7A07_003156"
     CDS             complement(8141..11362)
                     /gene="carB"
                     /locus_tag="N7A07_003156"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU2477774.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11380..12528)
                     /gene="carA"
                     /locus_tag="N7A07_003157"
     CDS             complement(11380..12528)
                     /gene="carA"
                     /locus_tag="N7A07_003157"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU2477775.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2945 a         3276 c         3317 g         3021 t
ORIGIN      
        1 caaactgatt aataatcaaa attaacgaga aaagcgccct ctattcaccc gaaacaaaaa
       61 tgtgataaca atcacagaat gcagcttatt gaatacccat tatgagttag ccattaacgc
      121 gtccatgagg ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc
      181 aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg
      241 gaatagaacc gaaggttttc tttccgccat taataatcgt cggcatactt tgttggctta
      301 cagtcagaga tctggatgcg gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca
      361 atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc
      421 tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accacctgaa tttagtaccg
      481 ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat
      541 cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag
      601 gagcgaaaga gttggggctg gcttacagct tgttcctctg gggacctctg ccgtgggcca
      661 cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga
      721 ttcgccccag ctccaccctg gtgccgctgg taggtgaaaa acacgccaaa gggttgttcg
      781 gcactatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg
      841 gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc
      901 tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgcct
      961 gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga
     1021 tgctgggttg ggtgttcatc gtcagcggtg ccagcttcat catgaactac ttcaccgatt
     1081 cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta
     1141 aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttacgcca
     1201 tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct
     1261 tcggcatggt gatgggactg acagcatcca cctggatcct gtggactgta ctcggtagta
     1321 acactctgct gttgatagat aaaaacatca tcaacattcc aaatctgatc gaacagtacg
     1381 gtgtggcgcg cgccatcatc gaaacctggg ccgctctgcc gctcagcacc gccaccatgt
     1441 ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata
     1501 ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaaccaccg ctgctggtgc
     1561 gtatcggttg gtcaattctg gttggcatta tcggtattgt tttgctggcg ctcggcggcc
     1621 tgaaaccgat tcaaactgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta
     1681 tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca
     1741 aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg
     1801 ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc
     1861 gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc
     1921 tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt
     1981 ggatggagct gggacgtctg ggggcaccaa cctatgtact gtaccagttg ccgggcgggt
     2041 tcaatacctt cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg
     2101 gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg
     2161 gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt
     2221 gttttattac cagtagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt
     2281 ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag
     2341 tgaccaaact tgagaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg
     2401 atgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca
     2461 aagaagagtt cgaccatgaa cgtttcctgg tagccctgac caactacggt acggcgatgt
     2521 gcgcctttga agatgcggcg cgctacgcca accagcgcgt gcagtttggc gaggctattg
     2581 gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga
     2641 aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg
     2701 cagcgatgtg caaatacttc tgcgccaatg cggcatttga agtggtggat agcgcaatgc
     2761 aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgacc
     2821 tgcgtgtaga ccgcgtttcc ggaggatctg acgaaatgca gatcctgacg ctgggtcgtg
     2881 cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgctg acgcgtctta
     2941 tcaggcctac gggagcacga atgtaggccg gataaggcgt ttacgccgca tccggcagtc
     3001 atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg
     3061 gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa attgccgggc cgttcgccgg
     3121 gcaaatgttc gcagaatggg gtgcggaagt tatctggatc gagaacgtcg cctgggccga
     3181 caccattcgc gttcaaccga actacccgca actctcccgc cgcaatttgc acgcgctgtc
     3241 gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga
     3301 tatcttcatc gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt
     3361 actgtggcaa cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg
     3421 caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct tcagtggtta
     3481 cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta
     3541 cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag cgcgtgaaac
     3601 cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta
     3661 cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga
     3721 tccctactat gccggttgcg gtctgtataa atgtgccgac ggctacatcg tgatggaact
     3781 ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcgg
     3841 tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc
     3901 actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga
     3961 acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag
     4021 caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac
     4081 ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg
     4141 aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa
     4201 cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt
     4261 cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg
     4321 caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg gcggacgttt
     4381 acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt
     4441 atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc
     4501 gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt
     4561 tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg
     4621 aaagtgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaattct
     4681 atcctatgta tcaacagatt cagcaagaag atgccactca attgcggcac atttgcctga
     4741 cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg aaaaatcaac
     4801 aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct
     4861 tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct
     4921 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg
     4981 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
     5041 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtacaga
     5101 agtaccgcgc caccattacc gaatgtattc cgatgatgat tcgtacgttg atggtgcagc
     5161 cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt
     5221 cggagcagga aaaagacaca ttttgtgaac gcttcggtgt tcgcttgctg acgtcttatg
     5281 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cgacgctggc
     5341 cgtcgattgg tcgggcgggg ttttgctacg acgcggagat ccgcgacgat cacaatcgcc
     5401 cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
     5461 tcaaagagta ttttctcaac ccgaaagcca ctgcaaaagt gctggaagct gatggctggc
     5521 tgcataccgg cgataccgga taccgcgacg aagaaggctt tttttatttc atcgatcgcc
     5581 gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca
     5641 tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg
     5701 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
     5761 ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca
     5821 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
     5881 aacggcaggg aactaccctg ccagctaata atcacgctct aaaacaatat gcattttttg
     5941 cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
     6001 atctgacccg caatggatca attctggaaa ttactcttga tcgcccaaaa gcgaatgcta
     6061 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
     6121 aattacgtgt cgccattatt accggtgccg gagagaaatt cttttccgcg ggctgggatt
     6181 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggttttgcgg
     6241 gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac ggctacgcct
     6301 ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca
     6361 gcttcgccct gccggaagcc aaactgggca tcgttcctga cagcggcggt gtgctgcgtc
     6421 tgccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg
     6481 gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga
     6541 tggataacgc ccgcgaactg gctcagcagc tggttaacag cgccccgctg gcgattgcgg
     6601 cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaatcg tatagctata
     6661 ttcgcagcgg cgtgttgaaa cactatccat cggttctgca ttcggaagat gccattgaag
     6721 ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag
     6781 ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag
     6841 tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
     6901 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
     6961 cattatgcat ggctacaccg acactgacac catcgttggg gaaaacggcc atatcgggca
     7021 cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt
     7081 gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa
     7141 agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg
     7201 cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
     7261 tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa
     7321 tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg
     7381 gcgtaaacgc cttatccaac tctgctggag agcaaccgta ggccggataa gatgcgccag
     7441 catcgcatcc ggcgaccagg tcgcacggta tcatcgaaat gacattaacg acgcatactc
     7501 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
     7561 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
     7621 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
     7681 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
     7741 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
     7801 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
     7861 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
     7921 cgttttgaag caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac
     7981 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
     8041 aaagatttac ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat tagttattcg
     8101 atcaaattag cggatgaaaa atatccgcca tgacacgcta ttatttgatc tgtgcgtgca
     8161 tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc gcggtagcga
     8221 aaccaccgtt cagggtggtg tcataatgca ctttatattg cagcgcactg cgacggatca
     8281 cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt gatgatgtag gtatattcgc
     8341 cattcttgat acggtcctga atgtgcggac ggccttcatg caccttgttt accagacgcg
     8401 gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc
     8461 cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg
     8521 aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa
     8581 acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagaacgc atttctggcc
     8641 ctaacagcgg gtcaacgccc gggaatttat tgaacggcag caccacttct ttcaccgagt
     8701 agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca
     8761 cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac
     8821 gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca
     8881 cgttcatcag gccgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatta
     8941 catcctgaat ttcctgactt aaggtgtacg ctggcagcga acatgcggag tcaccggagt
     9001 gcacgcccgc ctgctcgatg tgctccatga tgccgccaat cagcaccatt tcgccgtcgc
     9061 agatggcatc cacgtcaact tctactgcgt catcaaggaa atgatccagc aacactggcg
     9121 catcgttaga cacgctaacc gccgtctgga agtagcgacg caggtcagct tcgtcataga
     9181 cgatttccat cgcccgaccg ccgagaacgt aagacggacg caccaccagc gggtagccaa
     9241 tctcttttgc cttctcaact gccatttcaa tagcagtaac ggtggcgttc gccggttgtt
     9301 tcagtttcag acggtcaacc gcatgctgga agcgttcacg gtcttccgca cggtcgatag
     9361 catccgggct ggtgccgata accggtacgc cagcggcttc cagtgcacgc gccagtttca
     9421 gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt
     9481 ccagcacatc ttccagagtt accggctcga agtagaggcg atcggaagtg tcgtagtcgg
     9541 tagagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct tcacgcagcg
     9601 ccagcgaggc gtgtacgcag cagtagtcga attcgatacc ctgaccgata cggtttggac
     9661 cgccgccaag caccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt
     9721 cataagtgga gtacatgtaa gcggtgtcgg tggcaaactc tgccgcacag gtatccacgc
     9781 gcttgtagac cgggtgcagg tcatattggt cacgcagctt acggatttcc gcttcgcgta
     9841 cgcccgccag ttttgccaag cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga
     9901 attcagcgtt caggccagtg atgcccactt ccgccacttt ctcttccaga cgcaccagtt
     9961 cttcaatctg taccaggaac cagcggtcaa tgttagtcag gttgaagacg ccgtccacag
    10021 acaggcccgc gcggaaagca tcggcgatgt accagatacg ctctgcgcct gcgtctttca
    10081 gttcgcgacg gattttggtt aacgcttccg ggtcatccag gctcactttc gggtcgaatc
    10141 cagtcgcacc gacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac
    10201 caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccgg
    10261 cgaatttttc gaagttgaag cgaggaattt tggtaaccac gtaatcgatg gacggctcga
    10321 aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcatcgagg gtgtaaccca
    10381 ccgccagttt cgccgccact ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg
    10441 aagaacggga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca
    10501 ccgcaaactg gacattggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg
    10561 aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga
    10621 tggagtcacc ggtgtggatg cccatcgcat caaagttttc gatagagcag acgatgatgc
    10681 agttgtcgtt tttatcgcgc accacttcca tctcgtactc tttccagccg atcagcgact
    10741 catcaatcag caactctttg gtcggagaga gatccagacc gcgggcgcaa atttcttcaa
    10801 actcttcgcg gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa
    10861 taatgcacgg gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc gtatgtgcga
    10921 taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt
    10981 cttctgcttt atcaatcgca tcggcagtgg caccaatcat ggtgacgccg aactcttcca
    11041 acacgccctg acgttccagc tccagcgcgc agttcagcgc cgtctgaccg cccatggttg
    11101 gcagcaccgc gtccgggcgc tctttttcaa taatcttacg taccacttcc cagtgaatcg
    11161 gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc gggttggagt
    11221 tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc gcgccagagt
    11281 agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt
    11341 ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta
    11401 ctgctcaatt aactcgataa agtggtcgaa caacggcgcg gcgtcgtgtg gaccagggct
    11461 ggcttcaggg tgaccctgga agctgaacgc cggtttatcg gtgcgatgaa tgccctgtaa
    11521 cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc
    11581 caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcca catctttaac
    11641 cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag
    11701 cgccagcagc tgatgaccga ggcagatgcc gaagaccgga atatcggttt cgaggaattt
    11761 ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa
    11821 gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt
    11881 caggcgacag cctctatcca ccagcatccg caggatgttg cgtttggcac caaaatcata
    11941 agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa
    12001 cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc
    12061 catgccattc agacctggga acgcgcgggc tttttctaac gccagcgccg catccgggtt
    12121 atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt
    12181 acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagagaggtc
    12241 ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc
    12301 atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata
    12361 agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt
    12421 cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc
    12481 gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag
    12541 agaatattca ctcacttta
//
LOCUS       DBFIFS010000129        12526 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.129, whole genome shotgun sequence.
ACCESSION   DBFIFS010000129 DBFIFS010000000
VERSION     DBFIFS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.129"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..493)
                     /locus_tag="N7A07_003158"
     CDS             complement(<1..493)
                     /locus_tag="N7A07_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2477776.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RTVN"
     gene            complement(1101..2468)
                     /gene="rhlE"
                     /locus_tag="N7A07_003159"
     CDS             complement(1101..2468)
                     /gene="rhlE"
                     /locus_tag="N7A07_003159"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU2477777.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            2697..3368
                     /gene="cecR"
                     /locus_tag="N7A07_003160"
     CDS             2697..3368
                     /gene="cecR"
                     /locus_tag="N7A07_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU2477778.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            3371..4366
                     /gene="hlyD"
                     /locus_tag="N7A07_003161"
     CDS             3371..4366
                     /gene="hlyD"
                     /locus_tag="N7A07_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU2477779.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            4359..6095
                     /gene="ybhF"
                     /locus_tag="N7A07_003162"
     CDS             4359..6095
                     /gene="ybhF"
                     /locus_tag="N7A07_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU2477780.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            6088..7221
                     /gene="ybhS"
                     /locus_tag="N7A07_003163"
     CDS             6088..7221
                     /gene="ybhS"
                     /locus_tag="N7A07_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477781.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            7232..8338
                     /gene="ybhR"
                     /locus_tag="N7A07_003164"
     CDS             7232..8338
                     /gene="ybhR"
                     /locus_tag="N7A07_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477782.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(8300..8710)
                     /gene="ybhQ"
                     /locus_tag="N7A07_003165"
     CDS             complement(8300..8710)
                     /gene="ybhQ"
                     /locus_tag="N7A07_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU2477783.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            8843..9604
                     /gene="ybhP"
                     /locus_tag="N7A07_003166"
     CDS             8843..9604
                     /gene="ybhP"
                     /locus_tag="N7A07_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU2477784.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNTSASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9601..10842
                     /gene="clsB"
                     /locus_tag="N7A07_003167"
     CDS             9601..10842
                     /gene="clsB"
                     /locus_tag="N7A07_003167"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU2477785.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            10842..11798
                     /gene="ybhN"
                     /locus_tag="N7A07_003168"
     CDS             10842..11798
                     /gene="ybhN"
                     /locus_tag="N7A07_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU2477786.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11834..>12526)
                     /gene="ybhM"
                     /locus_tag="N7A07_003169"
     CDS             complement(11834..>12526)
                     /gene="ybhM"
                     /locus_tag="N7A07_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU2477787.1"
                     /translation="SNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYLEITATKY
                     DSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIVV
                     FSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERPIWVISCL
                     MIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAI
                     TLVWQRHTRFFH"
BASE COUNT         3001 a         3252 c         3275 g         2998 t
ORIGIN      
        1 aattaacagt cctcagaata tgcatctcaa tctgactgtc agaaagactt ttagaaaaac
       61 cactaccttc agtcagttca attttcatcc tgagtatttt atttttgagg tcgggaaatg
      121 tatttacata aggaataaaa acatctgatg aattagagtt gtttttcttt ccattaatat
      181 tatctattct aacggtgatc cctttctcat cacgaggaat tgcatcattc atatttatag
      241 agatcacatt ttcatgtctg gattgcccgg ttttaatcgt catattgata tcatttgcgc
      301 tgatctccga aatctcactg acgaattttt taaatgcatt atagcgctgg tggccagcat
      361 taaatttgcc aaatgaaaca taatatgcga aatgttcaag gaaaccatgt aattccagta
      421 aattaaccgg gtcagaaccc tgcaccatat ttaataacat cgtcgcataa ccattgcatt
      481 ttgaacaatc catacatcgc tcctttgcac ttgaatgatc tgcgaatcat aagcctggca
      541 aaatggggag tcttacagaa atcgctcatt tttaactttt cgtactgcaa attcaggatc
      601 aacctggtta tgaaagtttg ctgctgtacg gctctaaaca gactggcaat caatctttct
      661 atcaataagc tgaaatgagc gatttttgat agtttattag aactactaaa gatatacggt
      721 aatgaaacga ctgcacactt tattttgctt agggagatag tcggatacac tatagacaat
      781 gaaaataata aaatcaggga cagaatcaag ctaatactga atcacatatc tcataataat
      841 ttaccagatg atgaacaaac tctttatgca atagcatgtt tattccagat tttcccagaa
      901 tttgtttatg aatatcaacc aatgttatta acaataattc aaattgcaag gagaaataat
      961 ttcattgcca ttttagatac actagataga tcataaagca aatttattaa aacaataaaa
     1021 tcagacatgt catgcatgtc tgaccgtgtt acctgaataa tgggcaaaag cctgtgatac
     1081 tcttttgccc ttacatggta ttactgcgca gcggcaggtt tacgcggacg gcgacggcgt
     1141 tgttgttcgc ctgccggttt ggcatcgcca agacgacgag atggtttttc tgcaggcttc
     1201 gcgctagctg atttcgcacc accttctcca cggcgtggtt gctgttgacc gcgaccacca
     1261 ccaccttgcc cacgaccgcc gccgccacgt tgctggcgac cgttctggat cggttcggct
     1321 ttgattgacg ggtccggctc ataacccgga atcgcaatgc gcgggatctc ttttttcagc
     1381 agtttttcga tatcacgcag cagtttgtgt tcatcaacac acaccagcga caatgcttcg
     1441 ccagttgccg ccgcacggcc agtacgcccg atacggtgga cataatcttc cggtacgttt
     1501 ggcagttcat agttgacgac gtgcggcaac tcttcgatat ccagaccgcg cgcagcgatg
     1561 tcagttgcca ccagtacacg aatatcgccc gatttaaaat cggccagcgc acgggtacgc
     1621 gcaccttgcg atttattacc gtggatcgcc gcactacgga tgccatcttt attaagctgt
     1681 tcagcgagat ggttagcgcc atgtttggta cgggtaaaca ccagcacctg ctgccagttc
     1741 cctttcccaa tcatgtgcga cagcaattcg cgcttgcgtt tcttatcaac aaagtgaaca
     1801 tgctgggtca cctgatcaga cgcggtattg cggcgtgcca cttctatttc cagcgggttg
     1861 tgcaacagtt tttccgccag ggctttaata tcgtcagaga aggtcgcgga gaacaacagg
     1921 ttctggcgct tcgcaggtag ttttgttaac acgcgacgga tatcgtggat aaagcccatg
     1981 tcgagcatgc ggtcagcttc atcgaggacg aggatttcaa cctgatccag cttcactgca
     2041 ttctgatgtt ccaggtccag caaacgtccc ggcgttgcca ccagcacatc aacgccgccg
     2101 cgcaacttca tcatttgtgg gttaatgctg acaccgccaa acaccaccag cgaacgaatg
     2161 ttcaggtatt tgctgtaatc acggacgttt tcgccaatct gcgccgccag ttcacgggtc
     2221 ggggtaagaa tgagcgcacg taccggacga cgccctttgg cgtgcggctg gcgagtgatc
     2281 aggtgttgca acagcggcag cgtaaagccc gctgttttgc cggtgccggt ctgggcgcta
     2341 gccatcaggt cgcggccttc cagcaccgca gggatcgcct gctgctgaat aggggtgggt
     2401 tcacggtaac cctgctcggc aacggcgcgc aggatatcag ggcttaaacc caaagaatcg
     2461 aaagacataa ctactccgaa ccgccccgac cgttaccggt gtagttttca gggagatact
     2521 gaaaaggaaa atgacaaaaa ccacaatgtc atgagggagc ggagtgtagc agtttttgtg
     2581 acgcagcgca taaattatcc ctgtgactgg cgtagctaaa attcttagcc atactggaca
     2641 actcccatat ggagtcataa tcttaatcaa tcatttgatt aagaatggac tgtgcgatga
     2701 ataatcctgc catgacaatc aagggtgaac aggcgaaaaa acagctgatt gctgccgcac
     2761 tggcgcagtt tggtgaatat ggaatgaacg ccaccacccg cgagatagcc gcccaggccg
     2821 ggcagaatat cgctgccatc acctactact tcggttcgaa agaagatttg tacctcgcct
     2881 gcgcccagtg gattgccgat tttattggcg agcagttccg tccgcatgcc gaggaagccg
     2941 aacgcttgtt cgcccaacca cagcctgatc gggctgccat ccgtgaactg atccttcgcg
     3001 cctgcaggaa catgattaag ctgctcaccc aggatgacac cgtcaacctc agcaagttta
     3061 tctcccgtga gcagctctct cccacggcag cctaccacct ggtgcacgaa caggtgatta
     3121 gtccgctaca cagccacctg acacgcctga ttgccgcctg gaccggctgc gacgccaatg
     3181 acacccgcat gatccttcat acccatgcgt tgattggtga gattctggcg ttccgtcttg
     3241 gcaaagaaac aattctgtta cgtaccggct ggaccgcgtt cgatgaagaa aagaccgaac
     3301 tgatcaacca gacggtgacc tgtcatatcg acctgatttt gcaaggatta tcgcaaagga
     3361 gtttgtagtg atgaaaaaac ctgtcgtgat cggactggcg gtagtggtac ttgccgccgt
     3421 ggttgccgga ggctactggt ggtatcaaag ccgccaggat aacggcctga cgctgtatgg
     3481 caacgtggat attcgtacgg tgaatcttag tttccgtgtt ggggggcgcg ttgaatcgct
     3541 ggcggtggac gaaggtgatg ctatcaaagc gggccaggtg ctgggcgaac tggatcacaa
     3601 gccgtatgag attgccctga tgcaggcgaa agcgggcgtc tcggtggcac aggcgcagta
     3661 tgacctgatg cttgccgggt atcgcgatga agaaatcgct caggccgccg cagcggtgaa
     3721 acaggcgcaa gccgcctatg actatgcgca gaacttctat aaccgccagc aaggactgtg
     3781 gaaaagccgc accatttcgg caaatgacct ggaaaatgcc cgctcctcgc gcgaccaggc
     3841 acaggcaacg ctgaaatcag cacaggataa attgcgtcag taccgttccg gtaaccgtga
     3901 acaggacatc gctcaggcga aagccagcct cgaacaggcg caggcgcaac tggcgcaggc
     3961 ggagctgaat ttacaggact caacgttgat agcaccgtct gatggcacgc tgttaacgcg
     4021 cgcggtggag ccaggcacgg ttctcaatga aggtggcacg gtgtttaccg tttcactaac
     4081 gcgtccggtg tgggtgcgtg cttatgttga tgaacggaat cttgaccagg cccagccggg
     4141 gcgcaaagtg ctgctttata ccgatggtcg cccggacaag ccctatcacg ggcagattgg
     4201 tttcgtttcg ccgactgctg aatttacccc gaaaaccgtc gaaacaccgg atctgcgtac
     4261 cgacctcgtc tatcgcctgc gtattgtggt gaccgacgcc gatgatgcgt tacgccaggg
     4321 aatgccagtg acggtacaat tcggtgacga ggcaggacat gaatgatgcc gttatcacgc
     4381 tgaacggcct ggaaaaacgc tttccgggca tggacaagcc cgccgtcgcg ccgctcgatt
     4441 gcaccattca tgccggttat gtgacggggc tggtagggcc agacggtgcg ggtaaaacca
     4501 cgctgatgcg gatgctggcg ggattactga aacccgacag cggcaatgcc acggtgattg
     4561 gctttgatcc gatcaaaaac gacggcgcgc tgcacgccgt gctcggctat atgccgcaga
     4621 aattcggcct gtatgaagat ctgacggtga tggagaacct caatctgtac gcggatttgc
     4681 gcagcgtcac cggcgaggca cggaagcaga cttttgctcg cctgctggag tttacgtctc
     4741 ttggcccgtt taccggacgc ctggcgggca agctctccgg tgggatgaaa caaaaactcg
     4801 gcctggcctg taccctggtg ggcgaaccga aagtgttgct gctcgatgaa cccggcgtcg
     4861 gcgttgaccc tatctcacgg cgcgaactgt ggcagatggt gcatgagctg gcgggcgaag
     4921 ggatgttaat cctctggagt acctcgtatc tcgacgaagc cgagcagtgc cgtgacgtgt
     4981 tactgatgaa cgaaggcgag ttgctgtatc agggagaacc aaaagccctg acacaaacca
     5041 tggccggacg cagctttctg atgaccagtc cacacgaggg caaccgcaaa ctgttgcaac
     5101 gcgccttgaa actgccgcag gtcagcgacg gcatgattca ggggaaatcg gtacgtctga
     5161 tcctcaaaaa agaggccaca ccagacgata ttcgccatgc cgacgggatg ccggaaatca
     5221 acatcaacga aactacgccg cgttttgaag atgcgtttat tgatttgctg ggcggtgccg
     5281 gaacctcaga atcgccgctg ggtgcaatat tgcatacggt agaaggcaca cccggcgaga
     5341 cggtgatcga agcgaaagaa ctgaccaaga aatttggtga ttttgccgcc accgatcacg
     5401 tcaactttgc cgttaaacgc ggggagattt ttggtttgct ggggccaaac ggcgcgggca
     5461 aatcgaccac ctttaagatg atgtgcggtt tgctggtgcc gacttccggc caggcgctgg
     5521 tgctggggat ggatctgaaa gagagttccg gtaaagcgcg ccagcatctc ggctatatgg
     5581 cgcaaaaatt ttcgctctac ggcaacctga cggtcgaaca gaatttacgc tttttctctg
     5641 gtgtgtatgg cttacgcggt cgggcgcaga acgaaaaaat ctcccgtatg agcgaggcat
     5701 ttggcctgaa aagtattgcc tcacacgcca ccgatgaact gccattaggt tttaaacagc
     5761 ggctggcgct ggcctgttcg ctgatgcatg aaccggatat tctgtttctc gacgaaccga
     5821 cttccggcgt tgaccccctc acccgccgtg aattctggct gcacatcaac agcatggtag
     5881 agaaaggcgt cacggtgatg gtcaccaccc actttatgga tgaagcggaa tattgcgacc
     5941 gcatcggcct ggtgtaccgc gggaaattaa tcgccagcgg cacgccggac gatttgaaag
     6001 cacagtcggc taacgatgag caacccgatc ccaccatgga gcaagccttt attcagttga
     6061 tccacgactg ggataaggag catagcaatg agtaacccga tcctttcctg gcgtcgcgtg
     6121 cgggcgctgt gcgtcaaaga gacgcggcag atcgttcgcg atccgagtag ctggctgatt
     6181 gcggtagtga tcccgctgtt gctgctgttt atttttggtt atggcattaa cctcgactcc
     6241 agcaagctgc gggtcgggat tttactggaa cagcgtagcg aagcagcgct ggatttcacc
     6301 cacaccatga ccggttcgcc ctacatcgac gccaccatca gcgataaccg tcaggaactg
     6361 atcgccaaaa tgcaggcggg gaaaattcgc ggtctggtgg ttattcccgt ggatttcgcc
     6421 gaacagatgg agcgcgccaa cgctaccgca ccgattcagg tgatcaccga cggcagtgag
     6481 ccgaataccg ctaactttgt gcagggatat gtcgaaggga tctggcagat ctggcaaatg
     6541 cagcgggcgg aggataacgg gcagaccttt gagccgctta ttgatgtaca aacccgctac
     6601 tggtttaacc cggcggcgat tagccagcac tttattatcc ccggtgcggt gaccatcatc
     6661 atgacggtca tcggcgcgat tctcacctcg ctggtggtgg cgcgagaatg ggaacgcggc
     6721 actatggagg ctctgctctc tacggagatt acccgcacgg aactgctgct gtgtaagctg
     6781 atcccttatt actttctcgg gatgctggcg atgttgctgt gtatgctggt gtcagtgttt
     6841 attctcggcg tgccgtatcg cggttcgctg ctgattctgt tttttatctc cagcctgttt
     6901 ttactcagta ccctggggat ggggctgctg atttccacga ttacccgcaa ccagttcaat
     6961 gccgctcagg tcgccctgaa cgccgcgttc ctgccgtcga ttatgctttc cggctttatt
     7021 tttcagatcg acagtatgcc cgcggtgatc cgcgcggtga cgtacattat tcccgcacgt
     7081 tatttcgtca gcaccctgca aagcctgttc ctcgccggga atattccagt ggtgctggtg
     7141 gtgaacgtac tgtttttgat cgcttcggcg gtgatgttta tcggcctgac gtggctgaaa
     7201 accaaacgtc ggctggatta gggagaagag catgtttcat cgcttatgga cgttaatccg
     7261 caaagagttg cagtcgttgc tgcgcgaacc acaaacccgc gcgattctga ttttaccggt
     7321 gctgattcag gtgattctgt tcccgttcgc cgccacgctg gaagtgacca acgccaccat
     7381 cgccatctac gatgaagata acggcgagca ttcggtggag ctgacccaac gttttgcccg
     7441 cgccagcgcc tttacacatg tgctgctgct gaaaagccca caggagatcc gcccgaccat
     7501 cgacacacaa aaggcgttac tactggtgcg tttcccggct gacttctcgc gcaaactgga
     7561 taccttccag accgcgccgt tgcagttgat cctcgacggg cgtaactcca acagtgcgca
     7621 aattgccgcc aactacctgc aacagatcgt caaaaattat cagcaggagc tgctggaagg
     7681 aaaaccgaaa cctaacaaca gcgagctggt ggtacgcaac tggtataacc cgaatctcga
     7741 ctacaaatgg tttgtggtgc cgtcgctgat cgccatgatc accactattg gcgtaatgat
     7801 cgtcacttca ctttccgtcg cccgcgaacg tgaacaaggt acgctcgatc agctactggt
     7861 ttcgccgctc accacctggc agatcttcat cggcaaagcc gtaccggcgt taattgtcgc
     7921 caccttccag gccaccattg tgctggcgat tggtatctgg gcgtatcaaa tccccttcgc
     7981 cggatcgctg gcgctgttct actttacgat ggtgatttat ggtttatcgc tggtgggatt
     8041 cggtctgttg atttcatcac tctgttcaac acaacagcag gcgtttatcg gcgtgtttgt
     8101 ctttatgatg cccgccattc tcctttccgg ttacgtttcg ccggtggaaa acatgccagt
     8161 atggctgcaa aacctgacgt ggattaatcc tattcgccac tttacggaca ttaccaagca
     8221 gatttatttg aaggatgcga gtctggatat tgtgtggaat agtttgtggc cgctactggt
     8281 gataacggcc acgacagggt cagcggcgta cgcgatgttt agacgtaagg tgatgtaact
     8341 tcttatcttt cgccagcaaa gacactaccg ccgggccggc aaggattgcc agccctgcaa
     8401 ttgccagcaa aaagttgttt tccagtacgc gggacaacag ccacagcaca atcagcgctg
     8461 cgccaaaata ccaggtcgtg gtcagctctt ccagcacgtc tgccacctca cgccagcgtt
     8521 gctcggggtg gcgctgaaac accagcatca tcactaccgt gacacaatac agtgcgcata
     8581 atcccacgcc gacgtgaacc agagtcttac tcatccagcc caccaccagc agcgccacta
     8641 ccagtaaatg caacataatc tgccagcaac ttagacccgt tgcgacacga acacgttgtt
     8701 gccacttcat ggcttctctc ctgaagaatt tatctctact tactcagagt accgaacttt
     8761 acggaaaatt ccgtaagacc gcaccttttt gtgacgccga ctacactatt tcaatgagat
     8821 aagaggcaaa gacaggaaaa ttatgcccga tcaaacacaa caattttcgt tcaaggtgct
     8881 caccatcaat attcacaaag gctttaccgc gtttaaccga cgcttcattt tgccggaact
     8941 tcgcgatgcc gtgcgtaccg tcagcgccga tattgtttgc ctgcaggaag tgatgggcgc
     9001 gcacgaagtt catccgctgc atgtggaaaa ctggcccgat acctcgcact acgagtttct
     9061 cgccgacact atgtggagcg attttgccta cggtcgcaat gccgtatacc cggaagggca
     9121 tcacggcaac gccgtactgt cgcgttatcc cattgaacat tatgagaatc gcgatgtttc
     9181 ggtcgatggt gcggaaaagc gcggcgtgct ctactgccgc attgtgccgc cgatgaccgg
     9241 aaaagcgatt catgtgatgt gcgtacatct gggcctgcgt gaggcgcacc gtcaggcgca
     9301 gcttgcgatg ctcgccgaat gggtgaatga gctaccggac ggcgaaccgg tattggtggc
     9361 gggtgatttc aatgactggc ggcaaaaagc taatcatccg ttaaaagtgc aggccggact
     9421 ggatgagatt tttacccgcg cccacggacg cccggcgcgc acgtttccgg tgcaatttcc
     9481 tctactacga ctggacagga tctacgtcaa aaataccagc gccagcgcgc caaccgcgtt
     9541 gccgctgcgg acatggcgac acctttctga tcatgcccct ttaagtgcgg agattcattt
     9601 atgaaatgta gctggcgcga aggcaataag atccagttgc tggaaaacgg cgagcaatat
     9661 tatcccgcgg tgtttaaggc gattggcgag gcacaagaac gcatcattct tgaaacgttt
     9721 atctggtttg aggatgacgt cggcaaacaa ctgcatgcgg cactactggc agcagcgcaa
     9781 cgcggggtta aagcggaagt cttgctggat ggctacggtt cgccggatct cagcgatgag
     9841 tttgtcaatg aactgacggc agctggcgta gtgttccgct actacgatcc ccgccctcgc
     9901 ctttttggta tgcgcaccaa tgtgtttcgc cggatgcatc gcaaaattgt ggtaatcgac
     9961 gcgcgtatcg cctttattgg cgggctgaat tactccgccg agcatatgtc cagctacggt
    10021 ccagaggcta aacaggatta cgcggtacgc cttgaagggc cgattgtcga agatattctc
    10081 cagtttgagc tggaaaacct gcctggacag agcgcggcac gacgctggtg gcgacgtcat
    10141 cacaaagcgg aagagaaccg ccagccggga gaagcgcagg tattgctggt ctggcgcgat
    10201 aacgaagaac atcgcgatga tattgaacgt cactatctga aaatgctcac tcaggcgcgg
    10261 cgagaagtca ttatcgccaa cgcctacttc ttccccggct atcgattttt acacgccttg
    10321 cgtaaagcgg cacggcgcgg ggtgcggatc aaactgatca ttcagggcga accggatatg
    10381 ccgattgtca gagtcggtgc gcgcttgctg tataactatc tggttaaagg cggcgttcag
    10441 gtgtttgagt accgccgccg cccgctccac ggcaaagtgg cattgatgga cgatcactgg
    10501 gcgacagtag ggtccagtaa tctcgatccg ctcagtttgt cactgaatct cgaagcaaat
    10561 gtcatcatcc acgatcgtca ttttaaccag acgctgcgcg ataatctgaa cggcattatt
    10621 gccgcagatt gtcagcaggt cgatgagacc atgctgccga aacgcacctg gtggaaccta
    10681 accaaaagcg tgctggcgtt ccacttttta cgccacttcc cggcgctggt tggctggctt
    10741 ccggcacaca cgccacgtct ggcgcaggtt gatcctcccg cacaaccgac aatggaaacg
    10801 caggatcggg tagaaactga aaacacgggg gtaaaaccct gatgagtaaa tcacacccgc
    10861 gctggcgctt agcaaagaag atcctcacct ggctgttttt tatcgcggtg attgtgttgc
    10921 tggtggtcta cgccaaaaaa gtggactggg aagaggtctg gaaggtcatc cgcgactaca
    10981 atcgcgttgc gctgcttagt gcggtcgggc tggtggtcgt cagctatctg atttacggct
    11041 gctatgacct gctcgcccgc ttttactgcg gtcacaaact ggcgaagcgc caggtgatgc
    11101 tggtgtcgtt tatctgctac gccttcaacc tgacgctcag tacctgggtc ggcggcattg
    11161 gtatgcgcta tcgtttgtac tcccgtctgg gattgccggg cagcactatt acgcggattt
    11221 tctcgctcag tattaccacc aactggctgg gctatatttt actggcaggg attatcttta
    11281 ccgcaggcgt ggtggagttg ccggaccact ggtatgtcga tcaaactacg ctgcgtattc
    11341 tcggcattgg cttactgatg attatcgcgg tttatttgtg gttttgcgct ttcgcgaagc
    11401 accgccatat gaccatcaaa gggcaaaagc tggtgctgcc ttcgtggaaa ttcgccctcg
    11461 cccagatgct gatttccagc gttaactgga tggtcatggg ggcgattatc tggctgttac
    11521 ttggtcaaag cgtgaactat ttctttgtac tgggcgtgtt gctggttagc agtattgccg
    11581 gcgtcatcgt gcatgttccg gcggggattg gtgtgctgga agcggtgttt atcgcgctac
    11641 tggctgggga gcatacatcc aaaggcacaa ttatcgccgc cctactcgct taccgtgtgc
    11701 tgtattactt tatcccgctg ctgctggcgc tgatctgtta cctgttgctg gaaagccagg
    11761 cgaagaagct acgggcgaaa aatgaagcgg cgatgtgaag tttagttaag ttctttagta
    11821 tgtgcattta cgactaatga aaaaaacgcg tatgcctttg ccagacaagc gttatagcta
    11881 tcgcggcaag tatcccaaag aagccgataa aatagtaata gagtgttaat gcacctgtaa
    11941 aagcgcaacg aaccggactc cacaactctt cactatggca tttccccgcc aattcaagga
    12001 gcttatttcg tccatgcaag ataatgcctg accataacac aatcattaag caacttatta
    12061 cccaaatggg ccgttcactc attaatgcgg cattcacgat gattaccagg gccaaccctg
    12121 tcaacgtcat cataatcata aaacggtgag aaccaggatc aatattaaat aataaaccgg
    12181 ccagcatact gattaaaaac atcgcgcccg cagtgctgaa cacgacgata atggcatata
    12241 caggaactaa acttgcaacg acaatcgcca ccaaagagag taaaaacaga tatattacgc
    12301 ctgcgatggc ggcatttatc ttgttgatat ccttaattaa gccatacata caaagtaata
    12361 ataaccctat ggcgacatac attggtggtg aatcatattt ggttgccgta atttcgagat
    12421 acttagcccc ccaggccagc cctgcggttg ccaccaaacc taaagcggta atgagccata
    12481 taccgtttaa tatcctggtc tcgtgctgaa aatctaattt attgct
//
LOCUS       DBFIFS010000130        12286 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.130, whole genome shotgun sequence.
ACCESSION   DBFIFS010000130 DBFIFS010000000
VERSION     DBFIFS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.130"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            25..1077
                     /locus_tag="N7A07_003170"
     CDS             25..1077
                     /locus_tag="N7A07_003170"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU2477788.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1153..2586
                     /locus_tag="N7A07_003171"
     CDS             1153..2586
                     /locus_tag="N7A07_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU2477789.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2769..5030
                     /locus_tag="N7A07_003172"
     CDS             2769..5030
                     /locus_tag="N7A07_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU2477790.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5171..6454)
                     /locus_tag="N7A07_003173"
     CDS             complement(5171..6454)
                     /locus_tag="N7A07_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU2477791.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6606..7082)
                     /locus_tag="N7A07_003174"
     CDS             complement(6606..7082)
                     /locus_tag="N7A07_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU2477792.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(7141..8430)
                     /gene="bioA"
                     /locus_tag="N7A07_003175"
     CDS             complement(7141..8430)
                     /gene="bioA"
                     /locus_tag="N7A07_003175"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU2477793.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            8517..9557
                     /gene="bioB"
                     /locus_tag="N7A07_003176"
     CDS             8517..9557
                     /gene="bioB"
                     /locus_tag="N7A07_003176"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU2477794.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            9554..10708
                     /gene="bioF"
                     /locus_tag="N7A07_003177"
     CDS             9554..10708
                     /gene="bioF"
                     /locus_tag="N7A07_003177"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU2477795.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            10695..11450
                     /gene="bioC"
                     /locus_tag="N7A07_003178"
     CDS             10695..11450
                     /gene="bioC"
                     /locus_tag="N7A07_003178"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU2477796.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            11443..12120
                     /gene="bioD"
                     /locus_tag="N7A07_003179"
     CDS             11443..12120
                     /gene="bioD"
                     /locus_tag="N7A07_003179"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU2477797.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         2906 a         3102 c         3347 g         2931 t
ORIGIN      
        1 tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat gcgaggtgga
       61 acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac gcagcgcgat
      121 aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg tattggcggc
      181 ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc gcgtgctgat
      241 gttgattatc tgtttgctca agttatcgtc catgagaaac gtgtcgatac cacgcctaac
      301 tgcggcaata tgctgtctgg cgttggggca ttcgccattg aaaatggttt gattgcagcg
      361 acttcgccag ttacccgcgt acgtatccgc aacgtcaata cgggtacgtt catcgaagct
      421 gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat tgacggcgta
      481 ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg ccgctggaac gaaaaccgga
      541 aaagttttcc cgactgataa tcagattgat tattttgacg atgtcccggt gacctgtatc
      601 gatatggcga tgccagtcgt cattattccg gctgaatatc tgggaaaaac aggctatgaa
      661 ttaccggcgg aactggatgc cgacaaagca ttattagccc gcattgaatc tatccgtcta
      721 caagcgggta aagcaatggg cttaggcgat gtcagtaata tggttatccc taaacctgtg
      781 cttatttctc cagcgcagaa aggcggggca attaatgtgc gttattttat gccgcattct
      841 tgccatcgcg cgctggcgat aaccggtgct attgctattt ccagtagttg tgcattggaa
      901 ggtaccgtca cccgacaaat cgtcccttct gtaggatacg gcaatatcaa tattgaacac
      961 cccagtggtg ggctcgacgt tcatttaagt aatgaaggtc aggatgccac gacgttacgc
     1021 gcatctgtta ttcggacaac cagaaaaata ttttccggtg aagtttatct tccctgaaaa
     1081 gattcgttgt caggataagg acaatcaata aaggacttct gtatgagtca tacagaaaga
     1141 acaggatttt aaatgaataa gaaatcgtta tggaagctaa ttctgatatt agcgatccca
     1201 tgtattattg gctttatgcc agctccggca ggattaagcg aactggcgtg ggtgcttttt
     1261 ggtatttacc tggcggccat tgtggggctg gttatcaagc ctttcccgga acctgtcgta
     1321 ctgttaattg ccgttgctgc ctccatggtg gtggtcggta acttatccga cggtgcgttt
     1381 aagaccaccg ccgtattaag cggttactct tcaggtacta cctggctggt gttctcggcg
     1441 tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta aacgtattgc ctatctgctg
     1501 attggtaaaa tcggtaacac cacgctgggt ctgggttacg ttacggtatt cctcgatctg
     1561 gtattggctc cggcaacacc gtctaacacc gcgcgtgcgg gcggcattgt gttaccgatc
     1621 atcaacagcg tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga
     1681 cattacctga tgatgtccat ttacatggtc accaaaacca ccagctatat gttctttacc
     1741 gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg acattctgca cctgcaaatt
     1801 agctggggtg gatgggcgct ggccgccgga ttgccgggca tcattatgct gctggtcacc
     1861 ccgctggtga tttacaccat gtatccacca gaaattaaga aggtggataa caaaaccatc
     1921 gctaaagcgg gccttgccga actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt
     1981 gtctttgtgc tggcgctgct gggctggatt ttcagtaagt ctctgggggt tgatgaatct
     2041 tcagtggcta ttgttgttat ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac
     2101 gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt acggcggtat tatcggctta
     2161 agctccttat tatcgaaagt taaatttttc gaatggttag ctgaagtctt taaaaataac
     2221 ctggcatttg atggtcacgg taacgttgct ttcttcgtta ttattttcct cagcatcatc
     2281 gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc ggtatttgcc
     2341 atgctggcga acgtctccgg cgcaccgtta atgttaaccg cgctggcact gttgttctcc
     2401 aactcctatg gcggcatggt tactcactat ggcggcgcgg caggtccggt catctttggc
     2461 gtgggttata acgatattaa atcctggtgg ttggtcggtg cggtactgac gatattaacc
     2521 ttcctggtgc atatcaccct cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg
     2581 ctgtaaatat actcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     2641 gtcacttact tatgtaagct cctggggatt cactcgcttg tcgccttcct gcaactcgaa
     2701 ttatttagag tatatccatt tattttcttg ctgcgcactt cacggtgcgc agatatctgg
     2761 agcatttgat gatcaagtta tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa
     2821 ttgccgaaga acatttcacc ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg
     2881 cctggtctat tctctcttct cataatacgt ccggaaatat ggataaactt aaaattaagt
     2941 ttgattcatt agcctctcac gatattacct ttgttggtat tgtacagacc gctaaagcgt
     3001 ccggtatgga acgtttcccg ctgccgtatg tgctgaccaa ctgccataac tcactctgcg
     3061 ccgtcggcgg caccattaac ggtgatgacc atgtttttgg tttatcggca gctcagcgtt
     3121 atggcggtat ttttgtgcct ccgcatattg cggtcatcca tcaatatatg cgtgagatga
     3181 tggcaggtgg cggcaaaatg atcctcgggt cagacagtca cacccgttac ggtgcattag
     3241 ggacaatggc agtcggtgag ggcggcggcg agttggtaaa acaattgctt aatgacacct
     3301 gggatatcga ctatccggga gttgttgcgg tgcatctgac cggaaaacca gcgccgtatg
     3361 tggggccgca ggatgtggcg ttggctatca tcggtgccgt gttcaaaaac ggctacgtca
     3421 aaaacaaagt gatggaattc gtaggtcccg gtgttgctgc gctctctacc gatttccgta
     3481 acagcgttga cgttatgacc actgaaacga cctgtttaag ttctgtctgg caaaccgatg
     3541 aagaagtcca taactggctg gcgctgcacg gtcgcggcca ggattactgc cagcttaacc
     3601 ctcaaccgat ggcgtactac gatggctgca tcagcgttga tttaagcgcc atcaaaccaa
     3661 tgattgcgct gccgttccac ccgagcaacg tgtatgaaat cgacacactg aaccagaact
     3721 tgaccgacat tctgcgtgag attgaaattg agtccgaacg cgtggcgcac ggtaaagcca
     3781 aactctcgct gctggacaaa gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg
     3841 cgggctgttc tggcggtaac tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc
     3901 aatcctgtgg caatgacacc ttctcgctgg cggtttaccc gtcatcacag ccggtgttta
     3961 tggatctcgc caaaaaaggt gtggtagcag atttgattgg cgcaggcgca atcatcagaa
     4021 ccgcgttctg cggcccatgc tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta
     4081 ttcgccacac cacgcgtaac ttcccgaacc gcgaaggctc taagccagct aatgggcaga
     4141 tgtcagcggt ggcgttgatg gacgctcgtt ctatcgctgc gactgcggca aacggtggct
     4201 atttaacctc tgccagcgaa ctcgattgct gggacaacgt gccggagtac gccttcgatg
     4261 taacgccgta taaaaatcgc gtctatcaag gctttgtgaa aggggcggcc cagcaaccgc
     4321 tgatttacgg accgaacatt aaagactggc cggaattggg tgcgctgact gacaatatcg
     4381 tcctcaaagt gtgctcgaag atcctcgacg aagtgaccac caccgacgaa ttgattcctt
     4441 ccggtgaaac ctcttcttat cgttcaaatc cgattggtct ggcggagttt accctgtcac
     4501 gccgcgatcc aggttatgtt ggcagcagta aagcgactgc tgagctggaa aatcagcgtc
     4561 tggcggggaa tgtcagcgag ctgacagagg tgtttgcgcg cattaagcag attgctggtc
     4621 aggagcatat tgatccgctg caaactgaaa ttggcagcat ggtctatgcg gtgaaaccag
     4681 gcgatggttc tgcgcgtgaa caggcggcga gttgtcagcg tgtgattggc ggtctggcga
     4741 atattgccga ggagtacgcg actaaacgct atcgttctaa cgtcatcaac tgggggatgt
     4801 taccgctgca gatggtggaa gcgccaacct ttgaagtggg ggattacatt tacatccctg
     4861 gcattaaagc ggcgctggat aatccgggta cgacgtttaa aggttatgtg atccatgaag
     4921 atgcgccggt aacggaaatt acgctctata tggaaagtct gactgctgaa gagcgcgaga
     4981 ttatcaaggc gggtagtttg attaacttca ataaaaaccg tcagatgtaa aaagcgccat
     5041 gtgaatgtag gtcgcattcg gcacttattg tcggatgcga tgctggcgca tcttatccga
     5101 cctacaaatc gcatcgaacc gtaggccgga taaggcgttt acgccgcatc cggcaaatag
     5161 ttaatagctc ttacttcttc gcctctgcaa ccactttgct acccacgccg cggttattgt
     5221 attcccacat gcggttgtag ttagtgtcat tcagattgcg ctgtatttcg tcgctatcgt
     5281 caacgctgcc ggtgttaccc gcaaacggac gattagagat caccgcatcg ccccacggtt
     5341 tagccgtgtt aaaaccttcg ttgatggcgc tatcacggat caccacctga ccgttggtat
     5401 tggcatcaac atccagcgag cggcccagtt gtgccacgcc atcaccggaa gcattgaaac
     5461 ggctgtttac ggcgaggaaa ccgtagtaaa tgttggacag cgtagccggt gcaaacacat
     5521 acgcttcttg ctgagtacgt gagttcacca cgcggaattc ggtgttatcg aacaccactg
     5581 cgccgcgacc agaaacgata tccacatccc cttcaatgta gctgttggtc accagcgtac
     5641 gcggctgacg attcgtttcc agacggttct gcacaccgct gttggtgaca aagaaggtgt
     5701 tctgacgacc gagaatgtta acgttgttaa tctgtacctg gtcaccgtca gtacgcagtg
     5761 ccaccgccgg atggttaccc gcatctacgc tatcacccag cgtgttttcg atggtcaggt
     5821 tttgcagttg caggccattg ttttgtgacc agaagaccgc agaacacaga acaccgatac
     5881 tgtcgctgcg tttgctctgg cagctatcgt acatatacca cgctggttta cctggcatat
     5941 atttgccgcg cgggttgacg tcgtgacgcc agtcggcagg gctcatgccc ccatcaaggg
     6001 aaagcccaat cttcacatca atcggttttt cacctgtacc gtacagagta attccacctg
     6061 gagcagcagg gacatacacc gttccctgat actcaccagg catcacggca atatactggc
     6121 gcttgttggt gcgcttgata attgccgcat ctaccgccgc ctgaatcgtg gtatgcgtta
     6181 caccttgagt acccgccggg ccgacaacaa agtcaggttg cgcaggcagg gttatcgggg
     6241 acggattcca cgctgcagca cctggtgtca gggatgcaaa atagtgttga gcatcgaaat
     6301 tctgcgcttc ttttgccgac agaatcgggc gagaagaggt accaggcgca gtttgatcag
     6361 aaggacgttg atcgggtggt gttgagctac aggcggtcag cgtcacgcca aaagccaatg
     6421 ccagcgccag acgggaaact gaaaatgtgt tcacaggttg ctccgggcta tgaaatagaa
     6481 aaatgaatcc gttgaagtct gcttttttat actaacttga gcgaaacggg aaggtaaaaa
     6541 gacaaaaagt tgtttttaat acctttaagt gataccagat agcattgcgc catctggcag
     6601 agtgattaac taaacatcgc ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta
     6661 aacccgacca tcgcgccgct ggctccttca tcgacatcaa tacgctctac atccagcgcg
     6721 tgaacggtaa aaatgtagcg atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg
     6781 gttttaccaa aatcggtacg cgtctgcaaa acgccgtctg gcattgctac cagaccagag
     6841 ccaaaccctt gcggtaatac gcgggtatca gcgggtaaat taacaactac ccagtgccac
     6901 cagccggagc cggttggcgc atccgggtcg tagcaggtga caacaaaact tttcgttccc
     6961 gcaggaacat catcccacgc cagatgcggt gaaatattat cgccatcgta acccatgccg
     7021 ttaaagacat gacgatgcgg caacttatcg ccatcgcgca gatcgttact gatgagtttc
     7081 ataaaccctc ctttctagtt tgcagaaagt gtagccagaa actctcacgc tgacttcccg
     7141 ttattggcaa aaaaatgttt catcctgtac cgcgcggtta accgctgcgg tcagacgctg
     7201 caactgttgc gggagaataa tatagggcgg catcaggtaa atcagtttgc caaaaggccg
     7261 gatccagaca ccctgttcga caaagaattt ttgcaatgcc gccatattca ccggacgagt
     7321 tgtttcgact acgccaatgg cccccagcac gcgcacatcg gcaaccattt cggcatcacg
     7381 ggcgggggca agttgctcgc gcagctgtac ttcaatagcc gccacctgtt gctgccagtc
     7441 gccagattcg agaatcgcca ggctggcgtt tgcagccgca caggccagcg gattgcccat
     7501 aaaagttggc ccgtgcataa agcagccggc ttcgccgtta ctgatggttt ctgcaacctc
     7561 gcgcgtggtg agtgtggcgg aaagggtcat tgtgccgccg gttaaggctt taccgaggca
     7621 caaaatgtcc ggcgcgattt ctgcatgttc acaggcaaac agtttgccgg tacgaccaaa
     7681 tccggtggcg atctcgtcgg caatcagcaa gataccttcg cgatcgcaca ttttgcggat
     7741 tcgttttaac cattccggat ggtacatgcg catcccgcct gcgccctgga caatcggttc
     7801 aatgatcacc gctgcgattt catgacgatg cgccgccatc aggcgggcaa agcccaccat
     7861 atcgcgctca tcccattcac catccatgcg gctttgcggg gcgggagcaa acaagttttc
     7921 tggcagatag cctttccaca ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc
     7981 gccaaaggta tcgccatgat aaccattgcg gaaggtcaga aaatgctggc gcgcttcgcc
     8041 tttggcttgc cagtactgca acgccatttt catcgccact tccaccgcta cggaaccgga
     8101 gtccgcgaga aaaacgcatt ccagcggttg cggcgtcatt gccaccagtt tgcggcacag
     8161 ctcaatggct ggcgcatggg tgataccgcc aaacatcaca tgcgacatgg catcaatttg
     8221 cgacttcatc gccgcattaa gctgcgggtg attgtaaccg tggatcgccg cccaccagga
     8281 cgacataccg tcaaccaggc gtctgccgtc agacaaaatt agctcgcaac cttcggcgct
     8341 cgccaccgga taaaccggta gcggggaggt catggatgtg tatgggtgcc agatatggcg
     8401 ttggtcaaag gcaagatcgt ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt
     8461 taggtttaca agtctacacc gaattaacaa caaaaaacac gttttggaga agccccatgg
     8521 ctcaccgccc acgctggaca ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg
     8581 atctgctgtt tgaagcgcag caggtgcatc gccagcattt cgatcctcgt caggtgcagg
     8641 tcagcacctt gctgtcgatt aagaccggag cttgtccgga agattgcaaa tactgcccgc
     8701 aaagctcgcg ctacaaaacc gggctggaag ccgagcggtt gatggaagtt gaacaggtgc
     8761 tggagtcggc gcgcaaagcg aaagcggcag gatcgacgcg cttctgtatg ggcgcggcgt
     8821 ggaagaatcc ccacgaacgc gatatgtcat acctggaaca aatggtgcag ggagtaaaag
     8881 cgatggggct ggaggcgtgt atgacgctgg gtacgttgag tgaatctcag gcgcagcgcc
     8941 ttgcgaatgc cgggctggat tactacaacc ataacctcga cacctcgccg gagttttacg
     9001 gcaatatcat caccacacgt acttatcagg aacgccttga tacgctggaa aaagtgcgcg
     9061 atgccgggat caaagtctgc tcaggtggca ttgtgggttt aggcgaaacg gtaaaagatc
     9121 gcgccggatt attactgcaa ctggcaaacc tgccgacgcc gccggaaagc gtgccaatca
     9181 acatgctggt gaaggtgaaa ggtactccac tggcagataa cgatgatgtc gatgcctttg
     9241 attttattcg caccattgcc gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt
     9301 ctgccggacg cgagcagatg aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa
     9361 actcgatttt ctacggttgc aaactgctaa ccacgcccaa tccggaagaa gataaagacc
     9421 tgcaactgtt tcgcaaactg gggctaaatc cgcagcaaac tgccgtgctg gcaggggata
     9481 acgaacaaca gcagcgtctg gagcaggcac tgatgacccc ggacactgac gaatattaca
     9541 acgcggcagc actatgagct ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc
     9601 tgccgatgcc ctgcgtcgcc gttatccagt ggcgcaagga gccggacgct ggctggtggc
     9661 ggacgatcgc cagtatctga acttttccag taacgattat ttaggtttaa gccatcatcc
     9721 gcaaattatc cgtgcctgga agcagggtgc ggagcaattt ggcgtcggta gcggcggttc
     9781 cggtcacgtc agcggttata gcgttgcgca tcaggcgctg gaagaagaac tggccgagtg
     9841 gctgggctat tcgcgggcac tgctatttat ctctggtttt gccgctaatc aggcggtcat
     9901 tgccgcgatg atggcaaaag aggaccgcat tgtcgctgac cggcttagcc atgcctcatt
     9961 gctggaggct gccagtttaa gcccgtcgca gcttcgccgt tttgttcata acgatgtcac
    10021 tcatctggcg cgactgcttg cttccccctg tccggggcag caactggtgg tgacagaagg
    10081 cgtgttcagc atggacggcg atagtgcgcc actggcggaa atccagcagg taacgcaaca
    10141 gcacaatggc tggttgatgg tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg
    10201 gcgcggcacc tgctggttgc aaaaggtaaa accagaattg ctggtagtga cttttggcaa
    10261 aggatttggc gtcagcgggg ctgcggtgct ttgctccagt acggtggcgg attatctgct
    10321 gcaattcgcc cgccatctta tctacagcac cagtatgccg cccgctcagg cgcaggcatt
    10381 acgtgcgtca ctggcggtca ttcgcagtga tgagggtgat gcacggcgcg aaaaactggc
    10441 ggcactcatt acgcgttttc gtgccggagt acaggatttg ccgtttacgc ttgctgattc
    10501 atgcagcgcc atccagccat tgatcgtcgg tgataacagc cgtgcgttac aactggcaga
    10561 aaaactgcgc cagcaaggct gctgggtcac ggcgattcgc ccgccaaccg taccctctgg
    10621 tactgcgcga ctgcgcttaa cgctaaccgc cgcgcatgaa atgcaggata tcgaccgtct
    10681 gctggaggtg ctgcatggca acggttaata aacaagccat tgcagcggca tttggtcggg
    10741 cagccgcgca ctatgagcaa cacgcagagt tacagcgcca gagtgctgat gtcttactgg
    10801 caatgctccc acagcgtaaa tacccccacg tactggacgc ggggtgtggg cctggctgga
    10861 tgagccgcca ctggcgagag cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa
    10921 tgcttgcgca agcccgccag aaagatgctg cagaccatta tctggcggga gatatcgaat
    10981 ccctgccgtt agcgactgcg acgttcgatc ttgcatggag caatctcgcg gtgcagtggt
    11041 gcggtaattt atcaacggca ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg
    11101 tcgcgtttac cacgctggtg cagggatcgt tacccgaact gcatcaggcg tggcaggcgg
    11161 tggacgagcg cccgcatgct aatcgctttt taccgccaga tgaaatcgaa cagtcgctga
    11221 acggcgtgca ttatcaacat cgtattcagc ccatcacact atggtttgat gatgcgctca
    11281 gtgccatgcg ttcgctgaaa ggcatcggtg ccacgcatct tcatgaaggg cgcgaccagc
    11341 gaatattaac gcgttcacag ttgcagcgat tgcaactggc ctggccgcaa caacaggggc
    11401 gatatcctct gacgtatcat ctttttttgg gagtgattgt tcgtgagtaa acgttatttt
    11461 gtcaccggaa cggataccga agtggggaaa actgtcgcca gttgtgcact tttacaagcc
    11521 gcaaaggcag caggctatcg gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag
    11581 accccggagg gtttacgtaa tagtgacgcg ctggcgttac agcgcaacag cagcctgcag
    11641 ctggattacg caacggtaaa tccttacacc ttcgcagaac ccacctcacc gcacatcatc
    11701 agcgcgcaag agggcaggtc gatagaatca tcggtaatga gctccggatt acgcgcgctt
    11761 gaacaacagg ctgactgggt gttagtggaa ggcgctggcg gctggtttac gccgctttct
    11821 gacactttca cttttgcgga ttgggtaaca caggaacaac tgccggtaat actggtggtt
    11881 ggcgtgaaac ttggctgtat taatcacgcg atgttgacgg cacaggcgat acaacacgcc
    11941 gggctgactc tggcgggttg ggtggcgaac gatgtcacgc ctccgggaaa acgtcacgct
    12001 gaatatatga ccacgctcac ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg
    12061 cttgcagaaa atccagaaaa tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag
    12121 ccatcctgta tttggttaaa ttgcgagcga gatcgcgtct tcgattgact gcaatttaac
    12181 caattaaatt ctaaaataat cacgaaaaaa attttacttc cgcatcatgc ggcgaatgtg
    12241 ggaattgccc aggcggcggg ggataggggc tgcagacagt tatcca
//
LOCUS       DBFIFS010000131        12188 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.131, whole genome shotgun sequence.
ACCESSION   DBFIFS010000131 DBFIFS010000000
VERSION     DBFIFS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.131"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            96..719
                     /gene="nfeR"
                     /locus_tag="N7A07_003180"
                     /gene_synonym="yqjI"
     CDS             96..719
                     /gene="nfeR"
                     /locus_tag="N7A07_003180"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU2477798.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(873..2393)
                     /gene="aer"
                     /locus_tag="N7A07_003181"
     CDS             complement(873..2393)
                     /gene="aer"
                     /locus_tag="N7A07_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU2477799.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2811..4190
                     /gene="ygjG"
                     /locus_tag="N7A07_003182"
     CDS             2811..4190
                     /gene="ygjG"
                     /locus_tag="N7A07_003182"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU2477800.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4232..4564)
                     /locus_tag="N7A07_003183"
     CDS             complement(4232..4564)
                     /locus_tag="N7A07_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU2477801.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4783..5766
                     /gene="ebgR"
                     /locus_tag="N7A07_003184"
     CDS             4783..5766
                     /gene="ebgR"
                     /locus_tag="N7A07_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU2477802.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5950..9042
                     /gene="ebgA"
                     /locus_tag="N7A07_003185"
     CDS             5950..9042
                     /gene="ebgA"
                     /locus_tag="N7A07_003185"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU2477803.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            9039..9488
                     /locus_tag="N7A07_003186"
     CDS             9039..9488
                     /locus_tag="N7A07_003186"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU2477804.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9551..10984
                     /locus_tag="N7A07_003187"
     CDS             9551..10984
                     /locus_tag="N7A07_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2477805.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            11118..12188
                     /gene="ygjJ"
                     /locus_tag="N7A07_003188"
     CDS             11118..12188
                     /gene="ygjJ"
                     /locus_tag="N7A07_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU2477806.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
BASE COUNT         2949 a         3241 c         3073 g         2925 t
ORIGIN      
        1 agatgagtcc acatttaaaa atcattttta ttcttgcaag agtgctcaca tcagatataa
       61 attagatata tctaattaag caaaaggagg ctgatatgag ccatcatcac gaagggtgtt
      121 gtaaacatga aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg
      181 agcactgcgg acacggtcac cagcatgaac acggtcaatg ttgcggtggt cgccacggtc
      241 gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg
      301 atattctctc gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa
      361 cccaagggaa ttacacccca agcccgggcg tcatctaccc gacgctggat tttctgcagg
      421 agcagtcgct gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg
      481 aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca
      541 tcaaagcgcg ttgcgttggc gctgcgctgc gccagaaccc gcaaatgaag cgggcgctag
      601 ataattttaa agcggtgctg aatttacgcg tcaaccagag tgatatcact gatgcacaaa
      661 taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat
      721 cgccgcatcc gccagtggcg cggtgcaatt gccggatgcg acgcttgacg caccttatcc
      781 ggcctacacc cgctacacac cccgtaggcc tgataagatg cgccagcatc gcatcaggca
      841 tcgtgtatca aataccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc
      901 agtcggctgg cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctcg
      961 gcatttttct gggtgatgag gttaagctca tccactgcac gggtcaggct ggaaagacca
     1021 tcggcctgtt ccagcgttga atggctaatc tgggcaatca actgggtgac gtttttcacc
     1081 tgtgccacaa tatcttccat cgtacgtccg gcggcgtgta cctgctgcga accggattgc
     1141 accttatcag cactggcatc aatcagcttg cgaatatcgt tggcagcatt ggcgctgcgg
     1201 ctggctaaat gacgcacttc ccccgccacc accgcaaaac ctttgccctg ttcgccggca
     1261 cgcgccgctt ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta
     1321 atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg
     1381 atcaccgtgg tcatcgcctc cccgccctgc actgccgcat tactggcggt gattgacagt
     1441 ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg
     1501 gtcgccaccg tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg
     1561 gtgcctttcg ccagcgtctc actgccgttt ctgacactgg acacctggct tgagacatcg
     1621 ttaattaacc aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc
     1681 tcatcgctgc gattcagatg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc
     1741 tgacgggcga cattttctat cgggcggaca atctgccatt caaaacaggc gcttgccaac
     1801 aataccacta acgcacagag gatatacgtc accaccgggg cagcaacaaa ccaaagcatg
     1861 gccgccagca agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat
     1921 gaaggcagtt tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttaccg
     1981 gtacgtccgg cgttcagcgc tttgtacagc ggctccaccg ccgcgatctc ttcatccgtc
     2041 gcccgggtac gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca
     2101 ttggcccgca cccaataatg gtcaccattt ttgcggcgat ttttcacgat gccgctccag
     2161 ggctcccctt ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg
     2221 tgacgcacca tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc
     2281 acaaaagtgt cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg
     2341 gtatcgtccg ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg
     2401 tcctgtgcag gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg
     2461 ttatctttaa ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat
     2521 tcgttgattt aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa
     2581 aaatcaggca gttaatgccc tgaaagcgtg catttgcgca gcaattatca aatccatacc
     2641 cgacaaaaac cgtgcaaaat aacaacagat gttaacaaat agcattaaat attgcgcaaa
     2701 tgataaccac atttgtgttt atcccgattt tcgcgatcgc agccggagtg gcgcaatccc
     2761 tgcaatactt aaatcggtat catgtgatac gcgagcctcc ggagcatatt ttgaacaggt
     2821 taccttcgag cgcatcggct ttagcgtgca gcgcccacgc cctgaatctc attgaaaagc
     2881 gaacgctgga tcatgaggag atgaaagcac ttaaccgaga ggtgattgaa tacttcaaag
     2941 agcatgtcaa tccggggttt ttagagtatc gcaaatctgt taccgccggc ggggattacg
     3001 gagccgtaga gtggcaagcg ggaggtttaa atacgcttgt cgacacccag ggacaggagt
     3061 ttatcgactg cctgggaggt tttggaattt tcaacgtggg gcaccgtaat ccagttgtgg
     3121 tttccgccgt acagaatcaa cttgcgaaac aaccactgca cagccaggag ctactcgatc
     3181 cgttacgggc tatgctggcg aaaacccttg ctgcgctaac gccaggtaaa ctgaaataca
     3241 gcttcttctg taatagcggc accgagtccg ttgaagcggc gctgaagctg gcgaaggctt
     3301 atcagtcgcc gcgcggcaaa tttactttta ttgccaccag cggcgcgttc cacggtaaat
     3361 cacttggcgc gctgtcggcc acggcgaaat cgaccttccg caaaccgttt atgccactgc
     3421 tgccagggtt ccgtcatgtg ccgtttggca atatcgaagc catgcgcacg gctcttaatg
     3481 agtgcaaaaa aaccggtgat gatgtggccg cggtgatcct cgaaccgatt cagggcgaag
     3541 gtggcgtaat tctgccgccg ccgggctatc tcaccgccgt gcgtaagctg tgcgatgagt
     3601 tcggcgcact gatgatcctc gatgaagtac aaacgggcat ggggcgcacg ggcaagatgt
     3661 tcgcctgcga acatgagaac gtacagccgg acatcctgtg cctggcaaaa gccctcggcg
     3721 gcggtgtgat gccgattggc gcaaccatcg ccactgaaga ggtgttctca gttctgttcg
     3781 acaacccgtt cctgcatacc accacctttg gcggcaaccc gctggcctgt gcggcggcgc
     3841 tggcgaccat caatgtgttg ctggagcaga acttaccggc tcaggctgag caaaaaggcg
     3901 atatgttgct ggacggtttc cgtcaactgg cgcgggaata tcccgatctg gtacaggaag
     3961 cgcgtggtaa agggatgtta atggcgattg agtttgttga taacgaaatc ggctataact
     4021 ttgccagcga gatgttccgc cagcgcgtac tggtggccgg aacgctcaat aacgccaaaa
     4081 cgatccgcat tgaaccgcca ctgacactga ccattgaaca gtgtgaactc gtgatcaaag
     4141 cggcgcgtaa ggcgctggcg gccatgcgag taagtgtcga agaagcgtag tatcaaatcg
     4201 gatggcaata cgacgtcgcc atccgatctt tttatacgat acgtacgccc gcaggcatca
     4261 tccgctccgg cgttaacagc acgctttcac tgccgtcatc ggtttccgca cacaacagca
     4321 tgcattccga cgtttcacca cgcattttcg ctttttgcag attgcataag accaccaccg
     4381 tttttcccat caactcttct tcgctgtagt acggcaccag gctggtcacg gtttgcagtg
     4441 ttttttcccc cacatcaacc tgtacgatgt acagcttgtc ggcgttttca tggcgtttca
     4501 cttccacaat ctttccgacg cgcatttcca gacgtgcaaa atcagcgtaa gccacggttt
     4561 ccattgcttc ctcccttcga gtaaaatttt actaaactat agcaaaagtt tttctcaatc
     4621 ctgtaggcta aaaatggaga atgcaggcgt gatcacattc gtgagccgct gtgttaccgt
     4681 tacagcgtca aagaaacgcg ctttatttac tgaaaacagg tgacccgata agcacttcct
     4741 ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa acatggcaac actaaaagac
     4801 atcgcaatcg aagctggcgt atccctggcg acagtatcca gggtcttaaa tgacgatccg
     4861 acattgaatg tgaaagaaga gacgaaacat cgcattctcg agatcgccga aaagctggag
     4921 tacaagacca gtagcgcccg taaactacag acaggcgcag tcaaccaaca ccatattctg
     4981 gctatctaca gctaccagca ggagctggag atcaacgatc cttactatct ggcgatccgc
     5041 cacggcattg aaactcagtg cgaaaagctg gcgatcgaac tgaccaactg ttatgaacac
     5101 agcggattac cagacattaa aaacgtcacc ggtattttaa ttgtcggcaa acccacgccc
     5161 gccctgcgcg ccgctgccag cgcgttgacc gacaatatct gttttatcga ctttcacgaa
     5221 cccggcagcg gttacgatgc ggtggatatc gatctggcac gcatcagtaa agaaatcatc
     5281 gacttctata tcaaccaggg cgttaatcgt attggtttta ttggcggtga agatgagcct
     5341 ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg gccgactgaa acaagtggta
     5401 cgcgaagagg atatctggcg cggcggtttt tccagttcgt cgggttatga actggcaaaa
     5461 caaatgctgg cgcgggaaga ctatccgaag gcactgtttg ttgcttccga ttccattgct
     5521 atcggcgtac tgcgggcgat tcatgaacgt ggtctgaaca tcccacagga tatttcgctt
     5581 atcagcgtta acgatatccc caccgcgcga tttacctttc cgccgctctc caccgtgcgc
     5641 atccattccg aaatgatggg aagtcagggc gtaaacctgg tgtatgaaaa agcccgcgat
     5701 ggtcgcgcgc tgccgctgtt agtcttcgtt cccagcaaat taaaactgcg cggcacgacc
     5761 cgttaaatcc ccttacacac tgtccggcaa tcgtttttgc cgggcagtgc tgccgtttat
     5821 tttcgtgatc cagttaaagt aaatgcattt acccgctact ttttagtaaa aattttacta
     5881 aactccccag caattacaca aactaccatc accatgaatg gttccgattt ctctctaccg
     5941 ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc acgaaaaccg acttgcgccg
     6001 cgtgcgtact ttttttcata tgattctgtt gcgcaagcgc gtacctttgc ccgcgaaacg
     6061 agcagcctgt ttctgccctt aagcggtcag tggaatttcc acttttttga ccatccgctg
     6121 caagtgccag aagccttcac ctctgagtta atggctgact gggggcatat taccgtcccc
     6181 gccatgtggc aaatggaagg tcacggcaaa ctgcaatata ccgacgaagg ttttccgttc
     6241 cccatcgatg tgccgtttgt tcccagcgat aacccaaccg gtgcctatca acgtattttc
     6301 accctcagtg acggctggca gggtaaacag acgctgatta aatttgacgg cgtcgaaacc
     6361 tattttgaag tctacgttaa cggtcagtat gtgggtttca gcaagggcag tcgcctgacc
     6421 gcagagtttg acatcagcgc gatggttaaa accggcgaca acctgttgtg tgtgcgcgtg
     6481 atgcagtggg cggactctac ctacgtggaa gaccaggata tgtggtggtc agcggggatc
     6541 ttccgcgatg tttatctggt cggaaaacac ctaacgcata ttaacgattt cactgtgcgt
     6601 accgactttg acgaagccta ttgcgatgcc acgctttcct gcgaagtggt gctggaaaat
     6661 ctcgccgcct cccctgtcgt cacgacgctg gaatataccc tgtttgatgg cgaacgcgtg
     6721 gtgcacagca gcgccattga tcatttggca attgaaaaac tgaccagcgc cagctttgct
     6781 tttactgtcg aacagccgca gcaatggtca gcagaatccc cttatcttta ccatctggtc
     6841 atgacgctga aagacgccaa cggcaacgtt ctggaagtgg tgccacaacg cgttggcttc
     6901 cgtgatatca aagtgcgcga cggtctgttc tggatcaata accgttatgt gatgctgcac
     6961 ggcgtcaacc gtcacgacaa cgatcatcgc aaaggccgcg ccgttggaat ggatcgcgtc
     7021 gagaaagatc tccagttgat gaagcagcac aacatcaact ccgtgcgtac cgctcactac
     7081 ccgaacgatc cgcgttttta cgaactgtgt gatatctacg gcctgtttgt gatggcggaa
     7141 accgacgtcg aatcgcacgg ctttgctaat gtcggcgata tcagccgtat taccgacgat
     7201 ccgcagtggg aaaaagtcta cgtcgagcgc attgttcgcc atattcacgc gcagaaaaac
     7261 catccgtcga tcatcatctg gtcgctgggc aatgaatccg gctatggctg taacatccgc
     7321 gcgatgtacc acgcagcgaa ggcgctggat gacacgcgac tggtgcatta cgaagaagat
     7381 cgcgatgctg aagtggtcga tattatttcc accatgtaca cccgcgtgcc gctgatgaat
     7441 gagtttggtg aatacccgca tccgaagccg cgcatcatct gtgaatatgc tcatgcgatg
     7501 gggaacggac cgggcgggct gacggagtac cagaacgtct tctataagca cgattgtatt
     7561 cagggacatt atgtttggga gtggtgcgac cacgggatcc aggcgcagga tgacaacggc
     7621 aatgtctggt ataaattcgg cggcgactac ggcgactatc ccaacaacta taacttctgt
     7681 cttgatggtt tgatctattc cgatcagacg ccgggaccag gcctgaaaga gtacaaacag
     7741 gttatcgcgc cggtaaaaat ccacgcgctg gatctgactc gcggcgagct gaaagtcgaa
     7801 aataaactgt ggtttaccac gctggatgac tacaccctgc acgcagaggt gcgcgccgaa
     7861 ggtgaaacgc tcgcgacgca acagattaaa ctgcgcgacg ttgcgccgaa cagcgaagcc
     7921 cccttgcaga tcacgctgcc gcagctggac gcccgcgaag cgttcctcaa cattacggtg
     7981 accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac ttatcagttc
     8041 ccgctgaagg aaaacaccgc gcagcctgta cctttcgcac cgaataacgc ccgtccgctg
     8101 acgctggaag acgatcgttt gagctgtact gttcgcggct acaactttgc gatcaccttc
     8161 tcaaaaacga gtggtaaacc gacatcctgg caggtaaatg gcgaatcgct gctgacccgc
     8221 gagccaaaga tcaacttctt caagccaatg atcgacaacc acaagcagga gtacgaaggg
     8281 ttgtggcagc cgaatcattt gcagatcatg caggaacatc tgcgcgactt tgccgtagaa
     8341 cagagcgatg gtgaagtgtt gatcatcagc cgcacggtta tagcaccgcc ggtgtttgac
     8401 ttcgggatgc gctgtaccta catctggcgc atcgctgcag atggccaggt taacgtggcg
     8461 ctttccggcg agcgttacgg cgactatccg cacatcattc cgtgcatcgg tttcaccatg
     8521 gggattaacg gcgaatacga tcaggtggcg tattacggtc gtggaccggg cgaaaactac
     8581 gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga tgccatattc
     8641 gagaactatc ccttcccgca gaacaacggc aaccgtcagc atgtccgctg gacggcactg
     8701 actaaccgcc acggcaacgg tctgctggtg gttccgcagc gcccaattaa cttcagcgcc
     8761 tggcactata cccaggaaaa catccacgct gcccagcact gtaacgagct gcagcgcagt
     8821 gatgacatca ccctgaatct cgaccaccag ctgcttggcc tcggctccaa ttcctggggc
     8881 agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg ctttacgttg
     8941 ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga gttcggcgca
     9001 gggttctttt ccacaaattt gcacagcgag aataagcaat gaggatcatc gataacttag
     9061 aacagttccg ccagatttac gcctctggta agaagtggca acgctgcgtt gaagcgattg
     9121 aaaatatcga caacattcag cctggcgtcg cccactccat cggtgactca ttgacttacc
     9181 gcgtggagac agactccgcg accgatgcgc tatttaccgg gcatcgacgc tattttgaag
     9241 tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag acattacagg
     9301 tcgtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc ggagaaaccg
     9361 ttgaggtcga cgaagggcaa atcgttattt gcgatatcca tgaagcatat cggtttatct
     9421 gcaataacgc ggtcaaaaaa gtagttctca aagtcaccat cgaagatggt tatttccata
     9481 acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta ctcattttcg
     9541 gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct tgctctcgct
     9601 gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg agcttggact
     9661 ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct tctgtctgat
     9721 catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg cgtgggtaaa
     9781 aagttcgctg ggcggacgtt gggcatttat tactgcctat acctactggt tcgtaaacct
     9841 gttctttttc acctcgctgt tgccgcgcgt tattgcttat gcttcgtatg ccttcctcgg
     9901 ctatgaatat attatgacgc cggttgccac caccattatc agtatggtgc tgttcgcctt
     9961 ctccacctgg gtttccacca acggggcgaa aatgttgggg ccaattacct ccgtcacttc
    10021 aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg cgctggttgg
    10081 cggcgtacag cctgctgacc ccatcaccgt tgacgcgatg atcccgaact tcaactgggc
    10141 gttcctcggc gttaccacct ggatctttat ggccgcaggt ggcgcggagt ccgtcgctgt
    10201 gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca tcctcgccgg
    10261 gatttttatc ggtgtactat attccgtctc ctcggtgctg attaacgtct tcgtcagcag
    10321 caaagagttg aaatttactg gcggatcggt gcaggtattc cacggcatgg cggcgtattt
    10381 tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta ccgcaatgtt
    10441 cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt ttcttctccg aaatcccgga
    10501 aggcatcttt ggtaagaaaa ccgtcgaact taacgaaaac ggcgttccgg cgcgcgcagc
    10561 gtggatccag ttcctgatcg tcatcccgct gatgattatc ccgatgctcg gttccaatac
    10621 tgtgcaggat ctgatgaata ctattattaa tatgaccgcc gcagcgtcca tgcttccgcc
    10681 gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc tgccacgcga
    10741 tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga ttgcgatatt
    10801 tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga ccatcatttt
    10861 ttataacgtc ggcggtattg ttatattcct tggctttgcg tggtggaaat acagtaaata
    10921 tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca gcaatgttga
    10981 ttaaacagac taataaaaaa tgagcaggca ttttccctct tcctggatga ggagagggaa
    11041 aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac tttttcttta
    11101 gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta cttgctctgc
    11161 cgttttgcta cgccttttct gcggcaggag aagaagcacg tccggcagaa catgacgaca
    11221 caaaaacccc cgcaattacc tcgacatctt ctccttcatt tcgtttttac ggcgaattag
    11281 gggttggtgg atatatggat ttagagggtg agaataaaca taaatacagc gacggcacct
    11341 atattgaagg tggcctggag atgaagtacg gctcctggtt cggcctgatt tacggcgaag
    11401 gctggaccgt gcaggccgac cacgacggca atgcctgggt gcctgaccat agctggggcg
    11461 gtttcgaagg tgggattaac cgtttctatg gcggttatcg aaccaatgat ggcaccgaaa
    11521 tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg ggcgacttca
    11581 cacccgatct gggctacgtc attcccaata cccgcgacat tatgaccgcg ctgaaggtgc
    11641 agaacttaag cggcaacttt cgttatagcg tcaccgccac acctgccgga catcatgacg
    11701 aaagcaaagc atggctacat tttggcaagt acgatcgcta cgacgacaaa tacacctatc
    11761 cggcaatgat gaacggttac atccagtatg accttgccga aggcatcacc tggatgaatg
    11821 gtctggaaat taccgacggc acaggacaac tctatctcac gggactgctt acccctaact
    11881 ttgccgctcg cgcctggcac cataccggac gtgccgacgg gctggacgta ccgggaagtg
    11941 aaagcgggat gatggtgagc gccatgtatg aagcgttaaa gggcgtttat ctctccaccg
    12001 cttacaccta cgccaaacat cgccctgacc acgctgacga tgaaaccacc tctttcatgc
    12061 agtttggtat ctggtacgaa tatggcggcg gacgtttcgc aacggctttt gatagccgct
    12121 tctacatgaa aaatgcctct aacgatccca gcgaccaaat cttcctgatg caatatttct
    12181 actggtaa
//
LOCUS       DBFIFS010000132        12180 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.132, whole genome shotgun sequence.
ACCESSION   DBFIFS010000132 DBFIFS010000000
VERSION     DBFIFS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.132"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            238..1008
                     /locus_tag="N7A07_003189"
     CDS             238..1008
                     /locus_tag="N7A07_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU2477807.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            999..1472
                     /locus_tag="N7A07_003190"
     CDS             999..1472
                     /locus_tag="N7A07_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU2477808.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLGGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1579..2118
                     /gene="dnaT"
                     /locus_tag="N7A07_003191"
     CDS             1579..2118
                     /gene="dnaT"
                     /locus_tag="N7A07_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU2477809.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2121..2858
                     /gene="dnaC"
                     /locus_tag="N7A07_003192"
     CDS             2121..2858
                     /gene="dnaC"
                     /locus_tag="N7A07_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU2477810.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2907..3401
                     /gene="yjjA"
                     /locus_tag="N7A07_003193"
     CDS             2907..3401
                     /gene="yjjA"
                     /locus_tag="N7A07_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU2477811.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3655..5946
                     /gene="opgB"
                     /locus_tag="N7A07_003194"
     CDS             3655..5946
                     /gene="opgB"
                     /locus_tag="N7A07_003194"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU2477812.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6083..7105)
                     /gene="lgoD"
                     /locus_tag="N7A07_003195"
     CDS             complement(6083..7105)
                     /gene="lgoD"
                     /locus_tag="N7A07_003195"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU2477813.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7244..8158
                     /locus_tag="N7A07_003196"
     CDS             7244..8158
                     /locus_tag="N7A07_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU2477814.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8373..9735
                     /locus_tag="N7A07_003197"
                     /pseudo
     CDS             8373..9735
                     /locus_tag="N7A07_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9784..11448)
                     /gene="tsr"
                     /locus_tag="N7A07_003198"
     CDS             complement(9784..11448)
                     /gene="tsr"
                     /locus_tag="N7A07_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU2477815.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11677..>12180
                     /locus_tag="N7A07_003199"
     CDS             11677..>12180
                     /locus_tag="N7A07_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477816.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLK"
BASE COUNT         3012 a         3173 c         2922 g         3073 t
ORIGIN      
        1 tctattatcc tgacccgtaa aggctaaaac aataaattca ttatagtgat aatattcatg
       61 tgatagtttg tttgcggcga gagataattc gctttttatc accgagcttg tcccgataac
      121 caggattaat cagcgcaatt ttgatcttaa cctaccgcaa aacaaccttg cttcatgata
      181 caaatggatt ctcattatct gaacaatacg cagcacgtct atgacaaagg gcgagttatg
      241 caaactgagc aacagcgagc cgtaacacgg ctttgtatcc agtgtggatt atttcttttg
      301 caacatggtg cggaaagcgc gttggttgat gagctttcct cacgactggg tcgggcgctg
      361 ggaatggaca gcgtcgaaag ttctatctct tcgaacgcca tagtgctgac aactattaaa
      421 gatgggcaat gcctgacatc gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg
      481 gtgactgaag tccagcacat tgtgattctt gcggagcatc atctgctgga ttacaaaggc
      541 gtagagaaac gatttagcca aattcagcca ttacgttacc caagatggct ggtagcctta
      601 atggttggcc tttcttgcgc ctgtttctgt aaactcaata acggtggctg ggatggtgcc
      661 gtcatcacct tctttgccag tacgaccgcg atgtatatcc gccagctgct ggcacaacgt
      721 catcttcatc cacagatcaa cttttgcctt accgctttcg ccgccaccac catttccgga
      781 ttgcttttgc aacaccccac tttcagcaat acccccacca ttgcgatggc cgccagcgtt
      841 ctgctgctag tgccgggctt tccgttgatt aatgccgtcg ccgatatgtt taaaggccac
      901 atcaataccg gactggcacg ctgggcgatc gccagtctgc tgacactggc tacctgcgtc
      961 ggcgtagtga tggcactgac gatttggggg ctacgcggat gggtgtgatc gaatttctgt
     1021 tagcgttggc gcaggatatg atcctcgccg ccattcctgc ggtcggcttt gcgatggtgt
     1081 tcaacgttcc cgtgcgggcg ttacgctggt gtgcgctgct tggctcgata ggtcatggtt
     1141 cccgaatgat cttgatgacc agcgggttga atattgagtg gtcaaccttt atggcctcca
     1201 tgctgggcgg caccattggt attcaatggt cgcgctggta tctggcgcat ccgaaagtgt
     1261 ttaccgtggc ggccgttatc cctatgttcc cgggcatatc ggcttatacc gcaatgattt
     1321 cggcggtaaa aatcagccag ttaggttaca gcgaaccgtt gatgattacc ctgttaacca
     1381 actttcttac agcttcatcg attgttggtg cgttatccat cggtctttcc attcctggat
     1441 tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct cgcttaagca cacggatgag
     1501 agacagcctc ctctcctccg tgtgttacta taaaagttat ctcccttctc gttcatcgtt
     1561 ccatatttga gaaacagtat gtcttccaga gttttgaccc cggacgtcgt tggtattgac
     1621 gccctggtac acgatcacca aaccgttctg gcaaaagctg aaggcggtgt ggttgccgta
     1681 tttgctaaca atgccccggc gttttatgcc gtcacgcctg cacgcctggc tgaactgctg
     1741 gcgctggaag aaaagctggc gcgtccggga agcgatgtcg atctggacga tcaactctat
     1801 caggaaccgc aagccgctcc cgttgctgta cccatgggga aattcgccat gtatccggac
     1861 tggcaacccg atgccgactt tatccgcctg gcggctctat ggggcgtggc gctaagagag
     1921 ccggtaacca ccgaagaact ggcctcattc attgcctact ggcaggcgga aggtaaagtc
     1981 tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc tgcaaatcgg tcgtgccagc
     2041 aacggcggac tgccgaaacg agatgtgaat acggtcagtg aacctgacag ccaaattcca
     2101 ccaggattca gagggtaacg atgaaaaacg ttggcgacct gatgcaacgc ctgcaaaaaa
     2161 tgatgcctgc ccatatcaaa cctgcattca aaacgggtga agagctactg gcctggcaga
     2221 aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga aaatcgggcg atgaaaatgc
     2281 agcgcacctt taaccgctcc ggtattcgcc cactgcatca gaactgctcc tttgagaact
     2341 atcgcgttga gtgtgaaggg cagatgaatg cgttaagcaa agcgcgccag tatgtcgaag
     2401 agtttgacgg caacatcgcc agcttcatct tttctggtaa gccaggaacc ggcaaaaacc
     2461 atctggcggc ggcaatctgc aacgagctgc tgctacgcgg taaatccgta ttgatcatca
     2521 ccgtggccga tattatgtcg gcgatgaaag ataccttcag gaatagcggt accagcgaag
     2581 aacaactgct taacgatctg agcaacgttg atctgctggt gatcgatgag atcggcgtgc
     2641 agaccgaatc gaaatacgaa aaagtgatca tcaaccagat cgtcgatcgc cgctcttctt
     2701 ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga agagatgacc aagttgctgg
     2761 gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt gtgggtgatc ttcaactggg
     2821 atagctaccg tagccgtgta acaggtaaag agtattaatc cccgtttctg gcggtatact
     2881 gcgacacaat tccaccttga gtaatgatga tgaaaactgt taaacatctt ctgtgctgtg
     2941 ccattgccgc cagcgcatta atttccaccg gggtgcatgc tgcgtcctgg aaagatgcgc
     3001 tctccagcgc cgccagcgaa cttggcaacc aaaacagcac gacacaggaa ggcggttggt
     3061 cgctcgcgtc attaactaac ttgcttagca gcggaaacca ggccttaagc gcagataaca
     3121 tgaacaatgc cgcaggcatt ctgcaatact gcgcgaagca aaagctggct tcggtaaccg
     3181 atgtcgaaaa catcaagaac caggtgctgg aaaagctggg cctgaacagt gaagagcaaa
     3241 aagaagacac caactatctg gacggcattc agggtttgct gaaaaccaaa gatggtcagc
     3301 aactcaatct ggataacatc ggaacaactc cgctggcaga aaaggtgaaa accaaagcct
     3361 gcgatctggt gttaaagcag gggctgaact tcatttcctg aaccgcgaaa cgccgcgttt
     3421 tagctgttac ccggctgacg cggctctctc ctgccctttt ccctgctcct ggacggtttt
     3481 ccccctgtta cacgggaagc gaccgcgatc aaaaaagtct gtttataaac taattcctaa
     3541 caacatcgca tttttttgac actagaattg cagcaatatg gcgacgccat tacattgtgc
     3601 ttctcaaaaa gcttaaacgt tggctaaacg gcccggttta gtgaggatcg cccgttgtca
     3661 gaactactct ctttcgccct ttttctcgcc tctgtgctga tttacgcatg gaaagcggga
     3721 cgtaacacct ggtggtttgc agccacatta acggtgctgg ggctatttgt cgttttaaat
     3781 atcaccctgt ttgccagcga ctattttact ggcgatggta ttaacgacgc ggttctctat
     3841 accttaacca acagcctgac cggtgctggc gtcagcaaat acattttgcc gggtatcggc
     3901 attgtgctgg ggctgacagc ggtgttcggt gcgctgggct ggatcctgcg ccgtcgtcgc
     3961 catcatccgc accattttgg ttacagcctg ctggcgctct tactggcgct gggttcggtg
     4021 gacgccagcc cggcatttcg tcagataacg gaactggtga aatcccagtc acgcgacggc
     4081 gacccggact ttgcggctta ttataaagag ccgtcgaaaa ctatccctga cccgaaactc
     4141 aacctggttt atatctacgg cgaaagtctc gagcggacct attttgataa cgaggctttc
     4201 ccggacctca cgcctgaact gggcgcgttg aaaaatgaag gcctggattt cagccacacg
     4261 cagcagctgc cgggaacaga ttacacgatt gcgggcatgg tggcttctca gtgcggcata
     4321 ccgctgtttg ccccctttga aggcaacgcc tccgcttctg tttccagctt cttcccgcag
     4381 aacatctgtc tgggcgatat cctgaaaaac tcgggttatc agaactattt cgtgcagggc
     4441 gcgaatctgc gttttgccgg taaagatgtg ttcctgaagt cgcatggctt cgaccactta
     4501 tacggctcag aagagctgaa aagcgtggta gccgacccgc actatcgcaa cgactgggga
     4561 ttctacgacg ataccgttct cgatgaagcg tggaaaaagt ttgaagagct ttcccgctca
     4621 ggtcagcgat tctcactgtt taccctgaca gtcgataccc atcacccgga tggttttatc
     4681 tctcgtacct gtaaccgcaa aaaatatgat tttgacggta aaccgaatca gtcattcagc
     4741 gcggtaagtt gcagccagga gaatatcgcg acgtttatca acaaaatcaa agcgtcaccg
     4801 tggtttaaag atactgtcat cgtcgtctct tctgaccatt tagcgatgaa caacacggcg
     4861 tggaaatacc tcaataagca ggatcgcaat aacctgtttt ttgtcattcg tggcgacaag
     4921 ccgcagcaag agacgctggc agtgaagcgt aacacgatgg ataacggagc gacagtgctg
     4981 gacattctcg gtggcgataa ctatctcgga cttggtcgta gcagtttatc cgggcagtca
     5041 atgtcggaaa tcttcctcaa tatcaaagag aaaacattgg cgtggaagcc ggatatcatc
     5101 cgtctgtgga aattccctaa agagatgaaa gagttcacca tcgaccagca gaaaaacatg
     5161 attgccttct cgggtagcca tttccgtttg ccgctgctgt tgcgggtttc agacaaacgc
     5221 gtggaaccgc tgccagaaag cgaatactca gcaccgctgc gtttccagct ggccgatttc
     5281 gctccacgcg acaatttcgt ctgggttgac cgttgctaca agatggcaca actctgggct
     5341 ccggaactgg cactttccac cgactggtgt gtctcgcagg ggcaacttgg cggtcagcaa
     5401 attgttcagc atgttgacaa aacaacatgg aagagcaaga cggcatttaa agatacggtc
     5461 atcgacatgg cgcgttacaa aggcaatgtc gatacgctga agattgttga taacgatatt
     5521 cgctacaaag ccgacagttt catctttaac gtcgccggtg cgccagaaga ggtgaaacag
     5581 tttagcggga tttcccgtcc ggagtcgtgg ggccgctggt ccaacgcgca gctgggcgat
     5641 gaagtaaaaa tcgagtacaa gcatccgctg ccgaagaaat ttgacctggt gattaccgcc
     5701 aaagcatacg gcaataacgc cagccgtcct attccggtac gcgtaggcaa tgaagaacaa
     5761 actcttgtgc tgggcaatga agtgaccacc accacactgc atttcgataa cccaaccgat
     5821 gccgacacac tggtaattgt gccgccggaa cctgtctcaa ccaacgaagg gaatatcctc
     5881 ggacactcgc cgcgtaagct cgggatcggc atggtggaaa ttaaagtggt agaacgtgaa
     5941 gggtaattcc tgactgatgt gtaggtcgca tagggtgcga cctacacacc ttctccggct
     6001 tacaacacat tattaagaaa caaaaaaaac agcgcccaac acttccccgg acgctgcatg
     6061 gcaactttaa taatctcgcc tttcagaaag taattacgcc tttaattaac tcacgattgt
     6121 taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa cggatagcga tgggttaaca
     6181 tcatgccagc agtgattttt ccttccgcca tcagtcgacc gactttagca aaatcttccg
     6241 gcgtggcgtt gcggctgccc atcatcgtcg tttctttttt atggaattcc ggatcggaga
     6301 actgcaactc acctttaaac aggccgacaa ataccaccgt gccgccgtga cgaatcagat
     6361 tcacggtgtt attcatcgca tgttgattac ctgtcgcgtc gatcactttc tgcgccagcg
     6421 aaccaccaaa ctgcgcccgc agctgcgcgt caaaatcttc ggctgacggg tccagcacgg
     6481 gcaactccag acgcgttgcc acatgttcac ggcgcgccgg actggtatcc gccaccacca
     6541 cctgtgcgcc atcggcttta gcgattgccg ccgcgcccag accgattggc ccggccccga
     6601 ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg caccgcatga gcgctaatag
     6661 cgaaaggttc aatcaatgcg gccgcctgcg ggtcaatacc gtctgcgggc aaaatgttcg
     6721 ccaccggcac gctcaaatac tcactaaaac cgccatcctg atgcacgcca atgactgaaa
     6781 ttttttcaca gcaattggta cgcccgcttt tacacgccgg gcattgctga caggcaacat
     6841 aagggatcac agcaacttgc tgaccatttt taagatcagc aatattttta cccagcccaa
     6901 caatctcccc acatatttca tggcctaaaa cccgtggata actaaaaaat ggttgatttc
     6961 caccccaggc atgaatatcg gtaccgcaaa tcccgacaga cttaattttt attaatgctt
     7021 cattgtcacc cggaataggt atctcgcgtt gtttccagac taattctttc ggctgctggc
     7081 aaattaaaac attcatcgta gacatgattc ggctctccat tttttgttgc cttagatatc
     7141 aatgaaaatg aaaaatggca gcggaacgga aaatctgttt tgtgaaaaca cacgcataaa
     7201 aatgttttaa atcgggtttt aatggactca caaggaggaa aatatgagtc gttcacaaaa
     7261 tttacgccac aatgtgatta accaggtcat tgatgatatg gcgcggggtc atatcccttc
     7321 cccacttcca tcgcaaagtg cgctggcgga aatgtacaac atcagccgca ctacagtgcg
     7381 tcatattctc agccacttac gcgaatgcgg cgtcttaacg caggttggaa acgactatgt
     7441 tattgcgcgt aaacctgacc atgatgacgg tttcgcctgt actaccgctt caatgagtga
     7501 acaaaacaaa gtgtttgagc aggctttttt cacgatgata aaccagcgcc agttacgccc
     7561 aggggaaact ttttctgaac tgcaactggc tcgggccgcg ggtgtcagcc cggtggttgt
     7621 aagagaatat cttttaaaat tcggccgtta taatcttatt caaagcgaaa agcgcggcca
     7681 gtggagcatg aagcaattcg atcaatccta cgctgagcag ctgtttgagc tacgcgaaat
     7741 gctcgaaaca cattcacttc agcactttct caacctgcct gatcatgatc cccgctggct
     7801 gcaggcgaaa accatgctcg aacgtcatcg gctactgcgt gacaacatcg gcaatagctt
     7861 tcgcatgttc tcgcaacttg acagagattt tcactcctta ctgctttcag ctgccgacaa
     7921 tatctttttt gatcaatcgc ttgagatcat ctcagtcatc tttcattttc actatcaatg
     7981 ggacgaaagc gatcttaaac agcgcaatat cattgccgtc gatgagcata tgaccattct
     8041 cagcgctctg atttgccgca gtgaccttga tgccaccctt gcgctccgta accatttgaa
     8101 ttcagcgaaa caatcaatga ttcgatccat taatgagaac acgcgttatg ctcattaagt
     8161 acggattaaa aacagcaaac tattagcggg tgcacggttt tatcaaaaga taaataccgg
     8221 gaaattcccg cttacctatg ctcacaatca gtcgactaaa aatgtttttg tgagcggtag
     8281 taaagtccta aaactttaac ctgccgttaa tgtcaacctt gcgtaatgca caattacgta
     8341 aacaattaaa attataaata caggagtccg gcgtggaaaa agaaaatatc accctcgatc
     8401 cgcgttcttc atttactcca tcttcgtcgg cagatattcc cgtgccacca gatggattag
     8461 ttcaacgcag tacccgaatt aaacgcattc aaaccaccgc catgttgtta ttattttttg
     8521 cggcggtaat caattatctc gaccgcagtt cgctgtcggt agcaaattta acgattcgtg
     8581 aagaattggg attaagtgcc accgaaatcg gcgctttgct ctccgtgttt tcactcgctt
     8641 acggcattgc gcaacttcct tgcggcccac tattggatcg taaaggccca cgcctgatgc
     8701 tgggactggg gatgttcttc tggtcactgt tccaggccat gtctggcatg gtgcacagct
     8761 ttacgcagtt cgtgtgtcgt gcgtatcggt atggggattg gtgaagcgcc gatgaaccca
     8821 tgcggtgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg cccgatgggc
     8881 ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct ggcggcgatg
     8941 atgctggtga tgggctggcg cgggatgttt attaccattg gtgtactggg gatttttctc
     9001 gccatcggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac tgccgttgaa
     9061 caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag ttttgccgaa
     9121 tggcgcagcc tgttccgtaa ccgcacaatg tggggaatga tgctcggatt cagtggcatc
     9181 aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac agcctataac
     9241 ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg tgctgccggg
     9301 atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc tccgattaaa
     9361 agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac gctgatagta
     9421 ccacaagcga caacatccat gatggcggtt ctgctgattg gcatggcact gttctgtatt
     9481 cactttgccg gaacatcctg ctggggcttg atccacgtcg cagttgcttc tcgcatgact
     9541 gcgtcggtgg gcagtatcca gaactttgcc agcttcatct gcgcctcttt cgcgccgatc
     9601 attactggtt ttattgttga tacaacccac tcattccgtc tggcactaat catttgcggt
     9661 tgcgtcaccg cagcgggagc actggcgtac atcttcctgg ttcgtcagcc gatcaacgac
     9721 ccgcgtaaag attaaactcc ctatgcccga taacattttg cttatcgggc atttttatgg
     9781 cgattaaaac gtttcccagt tctccccgct atctgccact gccattttac gcggcgtagc
     9841 tggcgtcacg gtttttacca cagccgatgt ttcacgctgc tgttgctgct gttgctgaat
     9901 ccggaacact gccacagctt cggtcaggcg actggcctgc tcttccaacg cggcggcggc
     9961 agcggcagac tcttccacca gcgcagcgtt ctgttgagtt acccggtcca tctcagcaac
    10021 cgctaagcca acctgatcga taccacggct ctgctcatca gaagcagaag ctatttcgcc
    10081 cataatgtcc gtcacgcggg tcacagcact gacaatctcc gccattgttt ccccggcgct
    10141 ttcgaccagc gtagagccaa catccacttt ccccaccgag tcttcaatca ggcttttaat
    10201 ttcacgagcc gcctgggcgc tacgctgggc cagattacgc acttctcccg caaccaccgc
    10261 aaaaccacgt ccttgctcac ccgcacgcgc tgcttcaacc gccgcgttca aagccagaat
    10321 attggtctgg aaggcaatgc cgtcaattac gctgataata tcggcgattt tctgcgaact
    10381 ggtggagata tcacgcatag tctgcaccac gttatctacc actttgccgc cgcgttgcgc
    10441 cgtttcagaa gcacttaacg ccaggtggct ggcctggcgc gcattctcgg cgttctgttt
    10501 caccgttgcg gtgagttgct ccatgctggc tgccgtctct tccagcgaag cggcctgttg
    10561 ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt tcgctggcac cgctatagat
    10621 ggcattggcc ccgttgcgca catcaccgac ggtacgcatc agctctccct gcatatggcg
    10681 caaactctct gccagttgcc ccatctcatt agagccatcc acctcaatcg gtttcaccag
    10741 atcgccgcct gcaatatgac gaatgctgtc aatcaggcga ttcattggcg ctaccagcga
    10801 ggctttaata ccgaaccaga cggcgaagat gaccgccagt acgacgatca tcacgcccac
    10861 cagaatccac atcgcctggc tgtaggaggc attgttatcg ctgacggcga tatcatagag
    10921 ccgatcgttt tgctccatgt aagccacata ctgcttctcg aaaccgtcct gatatccctg
    10981 ggtcggctga tcaaagaact cgttgatttt gcctgcacct aacagttgga tcagctccgc
    11041 cagcgcattg tgataaatat cgtaattacg tttgatctct gccgctgcgg cggtgctctg
    11101 acgcgggtca cgcggcaacg cttcgtaatc tgcccagttt ttttccgcct gtttcaacga
    11161 aatactggca ctctgcatca gctcagcaac ggttgaaccg ctaccaatat tattctgatc
    11221 catcatgtag cggatacccg cgcggttgag ggtgttacgc gtctgcaaca acgcgaccca
    11281 gctgccattc agcgtggatt gctgctggcg aatggtttgt aaaacagtga aattttcttt
    11341 gtcattcttt aaggcattaa agaacagacc gcctgatgtc agttgtaaaa ggccaaaaac
    11401 ggccaaaacc agcagtaagc tggtcacgat cttgatacgt tttaacatgg tttctctttc
    11461 ctgtggaccg atgcaaaatt ttcggcctga aatgagaaaa ctttataggt agcgacttga
    11521 aactggttaa ttttacctca ctttttttaa tttttgttca aaaatacaat aaccacttat
    11581 tgatacattt tcttttctta tttttttcac acgaattgta tctattttga acgaaatatt
    11641 ttatagagtg tggaatttta agaaaggaca tctgaaatgt taaaccgtga cttcgatcat
    11701 ctctcaacgc tattacatct cttttttgaa cgtgaggata tttgccaaaa gcttaatatt
    11761 attgaccaaa acaatatcac cggttgggaa gtgtggttcc aggtagagtt tgctaatatg
    11821 ctctgctcaa cagaccatga atggtggcgt gaacaagcac tttcctgtga tatgcgcaaa
    11881 aaacctgagc gcccgacact acgcacagat ttcttactac gtaaaaaagg ctgggcacaa
    11941 gatagctata tcgcgcttga aataaaacaa aacagagacg cgaccagttg tgttaaaaat
    12001 atgatcgcag acttagaaaa aagcgcaaaa ataaaacgtt ccgaactcga tttacgcagt
    12061 ttctggacct tgggtatcac ccctatgata gacagggaaa ggctggactc tctcatcgat
    12121 tgctatttag atgagaaatt ttatcttact aaatctcgta aaaaacacgt tttacttaaa
//
LOCUS       DBFIFS010000133        12156 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.133, whole genome shotgun sequence.
ACCESSION   DBFIFS010000133 DBFIFS010000000
VERSION     DBFIFS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.133"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            38..907
                     /gene="yjeJ"
                     /locus_tag="N7A07_003200"
     CDS             38..907
                     /gene="yjeJ"
                     /locus_tag="N7A07_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU2477817.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1100..1453)
                     /gene="yjeI"
                     /locus_tag="N7A07_003201"
     CDS             complement(1100..1453)
                     /gene="yjeI"
                     /locus_tag="N7A07_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU2477818.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1591..3237)
                     /gene="groL"
                     /locus_tag="N7A07_003202"
     CDS             complement(1591..3237)
                     /gene="groL"
                     /locus_tag="N7A07_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU2477819.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3281..3574)
                     /gene="groS"
                     /locus_tag="N7A07_003203"
     CDS             complement(3281..3574)
                     /gene="groS"
                     /locus_tag="N7A07_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU2477820.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3850..5103
                     /gene="yjeH"
                     /locus_tag="N7A07_003204"
                     /pseudo
     CDS             3850..5103
                     /gene="yjeH"
                     /locus_tag="N7A07_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
     gene            complement(5122..5598)
                     /gene="fxsA"
                     /locus_tag="N7A07_003205"
     CDS             complement(5122..5598)
                     /gene="fxsA"
                     /locus_tag="N7A07_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU2477821.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5935..7371
                     /gene="aspA"
                     /locus_tag="N7A07_003206"
     CDS             5935..7371
                     /gene="aspA"
                     /locus_tag="N7A07_003206"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU2477822.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7489..8790
                     /gene="dcuA"
                     /locus_tag="N7A07_003207"
     CDS             7489..8790
                     /gene="dcuA"
                     /locus_tag="N7A07_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU2477823.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8906..9244
                     /gene="cutA"
                     /locus_tag="N7A07_003208"
     CDS             8906..9244
                     /gene="cutA"
                     /locus_tag="N7A07_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU2477824.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9220..10917
                     /gene="dsbD"
                     /locus_tag="N7A07_003209"
     CDS             9220..10917
                     /gene="dsbD"
                     /locus_tag="N7A07_003209"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU2477825.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAQLRDRQP"
     gene            10954..11529
                     /gene="yjdC"
                     /locus_tag="N7A07_003210"
     CDS             10954..11529
                     /gene="yjdC"
                     /locus_tag="N7A07_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2477826.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11636..11711
                     /locus_tag="N7A07_003211"
     tRNA            11636..11711
                     /locus_tag="N7A07_003211"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11669..11671,aa:Phe,seq:gaa)
     gene            11909..12068
                     /locus_tag="N7A07_003212"
                     /pseudo
     CDS             11909..12068
                     /locus_tag="N7A07_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2929 a         3139 c         2989 g         3099 t
ORIGIN      
        1 taattattta attgagaaat ggttagggag aacctacatg gccataagca tcaagggtgt
       61 taataccggt gttattcgca agagtaataa tttcattgca ctggcgctga aaattaaaga
      121 acctcgcaat aaagaatcgt tgttctttat gtctgtcatg gaactgcgcg acctgctgat
      181 tgcgctggaa agtcgtctgc atcaaaagca taaactggat gctgctgcac gccttcagta
      241 tgagcaagct cgcgataaag tcattaagaa aatggcagaa aacatcccag aaattctggt
      301 tgatgagctt aaaaatgccg atattaatcg tcgggttaac acgctggaac ttaccgataa
      361 tcaaggcgaa aatcttacct tcgtattaac gctacatgac ggtagcaaat gtgagttagt
      421 cgtcaatgag ttgcagattg aaatgctggc gcgagcaatc attcatgcaa ttaataatgc
      481 ggaaatgcgt gaactggcat tacgtattac ctcactgtta gatttcctgc cgttatatga
      541 cgttgactgc cagcaaaatg gcaatctgga atatgacacc tattcccaac cggaatggaa
      601 acataacttg ttcgaccatt atctggcggt gctttaccgc tttaaagatg aaagtggcaa
      661 agaacaattt agcggcgcag tggtgaaaac gcgtgaagca acgccgggta aagaaataga
      721 ggcgatcact cgccgaatgc ttgatttcag cccacgactg aaaaaactcg tgggtgtgcc
      781 atgtcaggtc tatgttcgca ccgtagcggc gaataacgcc caaccgctaa cccaggatca
      841 atgcttacgc gcgctgcatc acttacgtgt tcaatccacc agcaaaactg caccacaggc
      901 gaaataatct catctttgct aatgcgaggc tggagccatc cagcctgaaa tgactgataa
      961 cgtgatcgtt ggtcatgccg gatgcggcgt aaacgcctta tccggcctac aaattctcaa
     1021 gcattcaatc agttgcaatg ccttatgtag gcctgataag cgaagcgcat ctggcaatta
     1081 tacgtttgtc accatcacat cagttcgggc acttatatac ctggccgata atctggctat
     1141 ccgtcgggac aaaactggac aacattccct gcgacgggct actgatgcca taaatcacgt
     1201 taccgcccat tgcagccgcc tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc
     1261 cctcttctcc gtgttgcccg gaaagccagt tgctttgctt acctgtcgca gtaccaatca
     1321 gctggcactc tgcgcctggc tgctcgtcca caatgcgtac actctgaccg gcagcactca
     1381 attcgttgct ggagctacaa cccgccatca gtagcgcggc accgacaatc cctgctaagt
     1441 attttacgtg catgttattc cccataatca atgagctgga cgccactcgc gtcgtccgtg
     1501 tctgaatctt atactagaaa gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg
     1561 gtttgtttat ttctgcgagg tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc
     1621 acccatgccg ccagcagcgc ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat
     1681 gcattcggtg gtgatcatca ggccagccac agaagccgcg tactgcagag cagaacgggt
     1741 tactttggtt gggtccagga tacccatgtc gatcatgttg ccgtattctt cggttgctgc
     1801 gttgtaaccg tagttgccgt cgccgccttt aacggtgtta gcaacaacag acggttcttc
     1861 gccgcagttc aggacgatct gacgcagcgg agcttccatt gcacgcagtg caactttgat
     1921 acccacgttc tggtcttcgt tctgaccacg caggtcagcc agtttagacg ctacgcggat
     1981 cagcgcaaca ccaccaccag caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag
     2041 ggcgtcttca acgcgtgctt ttttctcttt catttcaact tcggtagcag cacctacttt
     2101 gataactgca acgccgcctg ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta
     2161 gtcagaagtt gcttcttcaa tctgctgacg gatctgagca acacggccct ggattgcagc
     2221 ttcttcgccc acgccatcga tgatggtggt ggtgtctttg ttgatcacaa cgcgtttagc
     2281 ctgacccagg tcttccaggg ttgctttttc cagctccata ccgatctctt cagagattac
     2341 ggtaccgcca gtcagggttg cgatatcctg cagcatagct ttacgacgat cgccgaagcc
     2401 cggagcttta actgcagcaa ctttcacgat gccacgcatg gtgttaacaa ccagagttgc
     2461 cagcgcttcg ccttctacat cttcagcgat gatcagcagc ggtttgcctg ctttggcaac
     2521 ggcttccaga accggcagca tttcgcggat gttggagatt ttcttgtcag ccagcaggat
     2581 gaacgggctt tccagttcta ctgcgccagt ttccggcttg ttgatgaagt aaggagacag
     2641 gtagccacgg tcgaactgca taccttcaac cacgtccagt tcgtcctgca gaccggtacc
     2701 gtcttcaacg gtgataacgc cttctttacc gactttgtcc atcgcttcag cgatcagttt
     2761 acctacggtt tcgtcggagt tagcggagat agtaccaacc tgagcaatcg ctttagagtc
     2821 agagcacggt acggacagcg ctttcagttc ttcaactgca gcggtaacag ctttgtcgat
     2881 accacgtttc aggtccatcg ggttcatgcc cgcagcaaca gctttcagac cttcagtgat
     2941 gatagcctga gccagtacgg ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt
     3001 agaggcaact tctttcacca tctgcgcacc catgttttcg aacttgtctt ccagttcgat
     3061 ttcacgagca acggaaacac catctttggt gatggtcggt gcaccgaaag atttatccag
     3121 aactacgtta cggcctttcg gaccgagggt aactttcact gcatctgcca gtacgtttac
     3181 gccgcgcagc attttcacac gagcgtcgtt accgaatttt acgtctttag ctgccattat
     3241 ctttattcct taaattcgta tgttcagtgt cgtgcgagga ttacgcttca acaattgcca
     3301 gaatgtcgct ttcggacatg atcaacactt cttcattgtc gatcttctcg gatttcacac
     3361 cgtagccatc gttgaaaata acgatgtcgc caactttcac atccagcggc ttcacttcgc
     3421 cattttcaag gatacggcca ttgccgacag ccagcacttc gccgcgggtg gatttagccg
     3481 ctgcagagcc ggtcagaacg atgccgccag cagatttagt ttcaacttct ttacgcttga
     3541 cgatcacgcg atcatgcaat ggacgaatat tcattgataa ctctcctttg agaaagtccg
     3601 tatctgttat gggtgacgcc ggagcttacg tggtttcccg gctggtgacc agagaaatgg
     3661 ggatgaggct tcgccccttc aagggggaaa aagaaaaaaa attctgatca gcacaaaatc
     3721 gggtgaaaac cctgattcac ctcacatttc atcgcaattt cttcatcgcc gtcataagcg
     3781 aatctgattg tgctaccatc gaaaatctac gcagttgccc aaaatttggg cgcaatcgga
     3841 catcaaccca tgagtggact caaacaagaa ctggggctgg cccagggcat tggcctgcta
     3901 tcgacgtcat tattaggcac tggcgtgttt gccgttcctg cgttagctgc actggtagcg
     3961 ggcaataaca gcctgtgggc gtggcccatt ttgattatct tagtgttccc gattgcgatt
     4021 gtgtttgcga ttctgggtcg ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt
     4081 atggcgtttg gttcgcggct tgagcgagtc accggctggc tgtttttatc ggtcattccc
     4141 gtgggtttgc ctgccgcgct acaaattgct gccggattcg gccaggcaat gtttggctgg
     4201 catagctggc aactgttgtt ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt
     4261 actcgcggtg ccagttccag tgcaaatcta caaacagtta ttgccggact tatcgtcgca
     4321 ctgattgtcg ctatctggtg ggcgggcgat atcaaacctg cgagtatccc cttccccgcg
     4381 ccaggaaata tcgaacttac cgggttattt gctgcgttat cagtgatgtt ctggtgtttt
     4441 gtcggtctgg aagcatttgc ccatcttgcc tcggaattta aaaatccaga acgtgatttt
     4501 cctcgtgctt tgatgattgg tctgctgctg gcaggattag tctactgggg ctgtacggta
     4561 gtcgtcttac acttcgacgc ctatggtgaa caaatggccg cggcagcatc gcttccaaaa
     4621 attgtagtac aactgttcgg tgtaggagcg ttatggattg cctgcgtaat tggctatctg
     4681 gcctgctttg ccagtctcaa catttatata cagagcttcg cccgcctggt ctggtcgcag
     4741 gcgcaacata atcctgacca ctacctggca cgcctctctt ctcgccatat cccgaataat
     4801 gccctcaatg cggtgctcgg ctgctgcgtg gtgagcacgt tggtgattca tgctttagag
     4861 atcaatctgg acgctcttat tatttatgcc aatggcatct ttattatgat ttatctgtta
     4921 tgcatgctgg caggctgtaa attattgcaa ggtcgttatc gactactggc ggtggttggc
     4981 gggctgttat gcgttctgtt actggcaatg atcggctgga aaagtctcta tgcgctgatc
     5041 atgctggcgg ggttatggct gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc
     5101 acatcatccg gcgtttcgac attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg
     5161 gtcatcatcc tttcgctggt actcaccatc aaaagtatta ccgccaccgg tcccggcgct
     5221 aaaaccgccg cctggcatgc gagaaaagcg caaatgcggc atcaacttca ctgtcagatg
     5281 cttttgcacc ggcggcaata aaagcagaag accgaggaag tcggtgaaaa agcccggtaa
     5341 taaaagcagc aaaccggcaa tgatcagcga aacactttta atcatctccg ccgctgggtt
     5401 ttcacccgcc gccatttttt gctgcatcag cacgaaattc ttaaagccct ggttacgtac
     5461 cagtgacata ccgataactg acgtaaatat aaccagcacg agagtcagca atacccccaa
     5521 tacatgggca acctgaataa agattgaaat ctcaatatag acataaagga aaatggcaat
     5581 aaaaggtaac cagcgcaaag gtttctcctg taatagcagc cggttaaacc cggctacctg
     5641 aatgggttgc gaatcgcgtt cagcttatat tgtggtcatt agcaaaattt caagatattt
     5701 gcgcaactat ttttggtagt aatcccaaag cggtgatcta tttcacaaat taataattaa
     5761 ggggtaaaaa ccgacactta aagtgatcca gattacggta gaaatcctca agcagcatat
     5821 gatctcggat attcggtcga tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac
     5881 atatattctg tgtgtttaaa gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca
     5941 aacaacattc gtatcgaaga agatctgttg ggtaccaggg aagttccagc tgatgcctac
     6001 tatggtgttc acactctgag agcgattgaa aacttctata tcagcaacaa caaaatcagt
     6061 gatattcctg aatttgttcg cggtatggta atggttaaaa aagccgcagc tatggcaaac
     6121 aaagagctgc aaaccattcc taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa
     6181 gtcctgaaca acggaaaatg catggatcag ttcccggtag acgtctacca gggcggcgca
     6241 ggtacttccg taaacatgaa caccaacgaa gtgctggcca atatcggtct ggaactgatg
     6301 ggtcaccaga aaggtgaata tcagtacctg aacccgaacg accatgttaa caaatgtcag
     6361 tccactaacg acgcctaccc gaccggtttc cgtatcgcag tttactcttc cctgattaag
     6421 ctggtagatg cgattaacca attgcgtgaa ggctttgaac gtaaagcggt cgaattccag
     6481 gacatcctga aaatgggtcg tacccagctg caggacgcag taccgatgac cctcggtcag
     6541 gaattccgcg ctttcagcat cctgctgaaa gaagaagtga aaaacatcca acgtaccgct
     6601 gaactgctgc tggaagttaa ccttggcgca acagcaatcg gtactggtct gaacacgccg
     6661 aaagagtact ctccgctggc agtgaaaaaa ctggctgaag ttactggctt cccatgcgta
     6721 ccggctgaag acctgatcga agcgacctct gactgcggcg cttatgttat ggttcacgga
     6781 gcgctgaaac gcctggctgt gaagatgtcc aaaatctgta acgacctgcg cttgctctct
     6841 tctggtccac gtgccggcct gaacgagatc aacctgccgg aactgcaggc gggctcttcc
     6901 atcatgccag ctaaagtaaa cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa
     6961 gtcatcggta acgacaccac tgttaccatg gcagcagaag caggtcagct gcagttgaac
     7021 gttatggagc cggtcattgg ccaggctatg ttcgaatccg ttcacattct gaccaacgct
     7081 tgctacaacc tgctggaaaa atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa
     7141 ggttacgttt acaactctat cggtatcgtt acttacctga acccgttcat cggtcaccac
     7201 aacggtgaca tcgtgggtaa aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt
     7261 ctggaacgcg gtctgttgac tgaagcggaa cttgacgata ttttctccgt acagaatctg
     7321 atgcacccgg cttacaaagc aaaacgctat actgatgaaa gcgaacagta atcgtacagg
     7381 gtagtacaaa taaaaaaggc acgtcagatg acgtgccttt tttcttgtga gcagtaactt
     7441 aaaaataaca atctaatatc aacttgttaa aaaacaagga aggctaatat gctagttgta
     7501 gaactcatca tagttttgct ggcgatcttc ttgggcgcca gattgggggg aataggtatt
     7561 ggttttgcag gcggattggg ggtgctggtt cttgccgcta ttggcgttaa acccggtaac
     7621 atcccgttcg atgtcatttc cattatcatg gcggttatcg ccgctatttc tgccatgcag
     7681 gttgctggcg gtctggacta tctggttcat cagacagaaa agctgctgcg ccgtaacccg
     7741 aaatacatca cgatcctcgc accgatcgtg acctatttcc tgactatctt tgctggtact
     7801 ggcaacatct ctctggcgac actgccagtt atcgctgaag ttgcgaagga acaaggcgtt
     7861 aaaccttgcc gtccgctgtc tactgcagtg gtatccgcgc agattgcgat caccgcatcg
     7921 ccaatctcag cggcagtggt ttacatgtct tccgtgatgg aaggtcatgg catcagctac
     7981 ctccatctgc tctccgtggt catcccgtcc accctgctgg cggttctggt gatgtccttc
     8041 ctggtcacta tgctgttcaa ctccaaactc tctgacgatc cgatttatcg caagcgtctg
     8101 gaagagggcc tggttgaact gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa
     8161 acgtccgtct ggctgttcct gctgggcgta gttggcgtgg ttatctatgc aatcatcaac
     8221 agcccaagca tgggtctggt tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc
     8281 atcatgctca gtgttgcaac tctgaccacc gttatctgta aagtcgatac cgacaacatc
     8341 ctcaactcca gcaccttcaa agcaggtatg agcgcctgta tttgtatcct gggtgttgcg
     8401 tggctgggcg atactttcgt ttccaacaac atcgactgga tcaaagatac cgctggtgaa
     8461 gtgattcagg gtcatccgtg gctgctggcc gtcatcttct tctttgcttc tgctctgctg
     8521 tactctcagg ctgcaaccgc aaaagcactg atgccgatgg ctctggcact gaacgtttct
     8581 ccgctgaccg ctgttgcttc tttcgctgcg gtgtctggtc tgttcattct gccgacctac
     8641 ccgacgctgg ttgctgcggt acagatggat gacacgggta ctacccgtat cggtaaattc
     8701 gtcttcaacc atccgttctt catcccgggt actctgggtg ttgccctggc cgtttgcttc
     8761 ggcttcgtgc tgggtagctt catgctgtaa tgaccaatcg cggggcgttc acgccccgct
     8821 ttctttctcg ccgactaaca tcctttcccc gtccgttgta tagtgacctc tctcttgcgg
     8881 ttccatctgt tcttgcgagg tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt
     8941 cgtggtgcta tgtaccgcac cggatgaagc gacagcccag gatttagccg ccaaagtgct
     9001 ggcggaaaaa ctggcggcct gcgcgacctt gatccccggc gctacctctc tctattactg
     9061 ggaaggtaag ctggagcaag aatacgaagt gcagatgatt ttaaaaacta ccgtatctca
     9121 ccagcaggca ctgctggaat gcctgaagtc tcatcatcca tatcaaaccc cggaacttct
     9181 ggttttacct gttacacacg gagacacaga ttacctctca tggctcaacg catctttacg
     9241 ctgatcctgc tactttgcag cacttccgtt tttgccggat tattcgacgc gccgggacgt
     9301 tcacaatttg tccccgtgga tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac
     9361 cttaatctga cctggcagat caaagacggt tactacctct accgtaaaca gatccgcatt
     9421 acgccggaac acgcgaaaat tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat
     9481 gagttttacg gcaaaagcga gatttaccgc gatcggctga cgcttcccgt aaccatcaac
     9541 caggcgagtg cgggagcgac gttaactgtc acctaccagg gctgtgctga tgccggtttc
     9601 tgttatccgc cagaaaccaa aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg
     9661 tcacagcctg tgtctgttcc gcagcaagag cagcccaccg cgcaattgcc cttttccgcg
     9721 ctctgggcgt tgttgatcgg tattggtatc gcctttacgc catgcgtgct gccaatgtac
     9781 ccactgattt ctggcatcgt gctgggtggt aaacagcggc tctccactgc cagagcattg
     9841 ttgctgacct ttatttatgt gcaggggatg gcactgactt acacggcgct gggtctggta
     9901 gttgccgccg cagggttaca gttccaggcg gcgctacagc acccatacgt gctcattggc
     9961 ctcaccatcg tctttacctt gctggcgatg tcaatgtttg gcttgcttac tctacaactc
    10021 ccctcctcgc tgcaaacgcg cctcacgctg atgagcaatc gccaacaggg cggctcaccc
    10081 ggcggagtgt ttattatggg gacgattgcc ggactgatct gttcaccatg caccaccgca
    10141 ccgcttagcg cgattctgct gtatatcgcc caaagcggga acatgtggct gggcggcggc
    10201 acgctttatc tctatgcgtt gggcatgggc ctgccgctga tgctaattac cgtctttggt
    10261 aaccgcttgc tgccgaaaag cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt
    10321 gtgatcctcg cactgccggt cttcctgctg gagcgagtga ttggtgatgt atggggatta
    10381 cgcttgtggt cggcgctggg tgtcgcattc tttggctggg cctttatcac cagcctacag
    10441 gccaaacgcg gctggatgcg cgtggtgcaa ataatcctgc ttgcagcggc attggttagc
    10501 gtgcgcccac ttcaggattg ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat
    10561 ctcaacttta cacaaatcaa aacggtagat gagttaaatc aggcgctcgt tgaagccaaa
    10621 ggcaaaccgg tgatgttaga tctttatgcc gactggtgcg tcgcctgtaa agagtttgag
    10681 aaatacacct tcagcgaccc gcaggtgcaa aaagcgttag cagacacggt attacttcag
    10741 gccaacgtca ctgccaacga cgcacaagat gtggcgctgt taaagcatct taatgtcctt
    10801 ggcctaccga caattctctt ttttgacgga caaggccagg agcatccaca agcacgcgtc
    10861 acgggcttta tggatgctga aaccttcagc gcacaattgc gcgatcgcca accgtgaacg
    10921 acacttgcag tgggaaagac ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa
    10981 gccctgaaat tattagaatt acaagggatt gccaacacca cgctggagat ggttgctgaa
    11041 cgtgtggatt atccactgga cgagctacgc cgcttctggc cagacaaaga ggcaatcctc
    11101 tacgatgcgc tgcgttatct tagccaacag atagatgtct ggcgtcgtca gctgatgctg
    11161 gacgaaacgc aaaccgccga acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc
    11221 gttaaaaaca accgctatcc gggctgtctg tttatcgctg cctgtacgtt ttatcccgat
    11281 cctggccacc ctattcatca actggccgat cagcaaaaaa gcgcggccta cgatttcacc
    11341 cacgaactgt taaccacgct ggaagttgac gatccggcga tggtagcaaa gcagatggaa
    11401 ctggtgctgg aaggctgttt aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac
    11461 accgcacatc ggctggcgga agatatcctg cgcttcgccc gctgccgcca gggtggtgca
    11521 ctgacctgac agaaacacag aaaagaagcg atttgccgca atcttaagca gttgaatcgc
    11581 ttttactgaa attaggttga cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg
    11641 gatagctcag tcggtagagc aggggattga aaatccccgt gtccttggtt caattccgag
    11701 tccgggcacc aaattcatat aaacggacct ccacggaggt ccgtttttcg tttcaggacg
    11761 ccacgattta agtgttctgc ctccaaatca attctaccga actcaacccg attctcccca
    11821 catcaccagc aatttgcggg catatcccaa ttcgggaaaa tttgttcctg agctatagca
    11881 ctaactaacg tgaaatgtcg tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc
    11941 aggagcgtaa cgcatccata aagcgcaaca tcggtctgaa cgccgactga tgagattttc
    12001 tgaatgagaa caaagagaaa tgtatcagtc cgcttgctca tgcaaagact aacaatccat
    12061 taaaatagta agcgctccgg acaattttcc atggattatt ttctgaacat ttttctttgg
    12121 caaagatgat gaattttgat ggtaaggaat attact
//
LOCUS       DBFIFS010000134        12085 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.134, whole genome shotgun sequence.
ACCESSION   DBFIFS010000134 DBFIFS010000000
VERSION     DBFIFS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.134"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(28..312)
                     /gene="gatB"
                     /locus_tag="N7A07_003213"
     CDS             complement(28..312)
                     /gene="gatB"
                     /locus_tag="N7A07_003213"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU2477827.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(343..795)
                     /gene="gatA"
                     /locus_tag="N7A07_003214"
     CDS             complement(343..795)
                     /gene="gatA"
                     /locus_tag="N7A07_003214"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU2477828.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(805..2067)
                     /gene="gatZ"
                     /locus_tag="N7A07_003215"
     CDS             complement(805..2067)
                     /gene="gatZ"
                     /locus_tag="N7A07_003215"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU2477829.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(2096..2950)
                     /gene="gatY"
                     /locus_tag="N7A07_003216"
     CDS             complement(2096..2950)
                     /gene="gatY"
                     /locus_tag="N7A07_003216"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU2477830.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(3249..4301)
                     /gene="fbaB"
                     /locus_tag="N7A07_003217"
     CDS             complement(3249..4301)
                     /gene="fbaB"
                     /locus_tag="N7A07_003217"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU2477831.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            4558..5835
                     /locus_tag="N7A07_003218"
     CDS             4558..5835
                     /locus_tag="N7A07_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU2477832.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            5832..6836
                     /locus_tag="N7A07_003219"
     CDS             5832..6836
                     /locus_tag="N7A07_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU2477833.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            6833..7798
                     /locus_tag="N7A07_003220"
     CDS             6833..7798
                     /locus_tag="N7A07_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU2477834.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(7772..8518)
                     /locus_tag="N7A07_003221"
     CDS             complement(7772..8518)
                     /locus_tag="N7A07_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2477835.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8570..9388)
                     /locus_tag="N7A07_003222"
     CDS             complement(8570..9388)
                     /locus_tag="N7A07_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU2477836.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(9453..10253)
                     /gene="thiD"
                     /locus_tag="N7A07_003223"
     CDS             complement(9453..10253)
                     /gene="thiD"
                     /locus_tag="N7A07_003223"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU2477837.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(10250..11038)
                     /gene="thiM"
                     /locus_tag="N7A07_003224"
     CDS             complement(10250..11038)
                     /gene="thiM"
                     /locus_tag="N7A07_003224"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU2477838.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(11073..11169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11372..11611
                     /locus_tag="N7A07_003225"
     CDS             11372..11611
                     /locus_tag="N7A07_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HGU2477839.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         2939 a         3090 c         2960 g         3096 t
ORIGIN      
        1 ataacgcatg acttctgaaa acataggtca cccctgtaag atagtcagaa ttttattttg
       61 taatgcttcg ataccgacac cagaaacaaa aggcatgccg tgaactaacg gaatatcgcc
      121 aaaactacga tccaccctgg cagtggtgca tatcaaatgc acgccatcca tataggtttc
      181 tatttcatta acccgacact ggattaattc aacaggaata ttatgactct gacacaactc
      241 tttaatttct tccgccgcca tcgtagaggt cgcaaccgcg cctccgcaag cgacaataat
      301 cttgcgtttc ataaatagtc ctttttattg ttacagaggg atttattcgt ctgaatctaa
      361 aacatacttt gtgaagtatt cctttaactg agtttcagga agagtgatta gtgtctcaac
      421 gatatcgggc tgttgtaact tgccaaataa acagcgtaaa agtttcaatt gctgctgcgg
      481 attttccaca atcaacgcaa taaccaacga taccgccacg tcattatcat catccgcttg
      541 ctgaaaatga actttatttg ttggccttaa cagataaatg gctgacgact tagcatgaat
      601 cgcctcacaa tgcggtattg caatggcgtg ctgctcaagc attatcccgg tagggaattc
      661 tgcttctctg gcaattaatg cctgtggcca ggtatcatga accacacctt tggcgagcat
      721 ctcattaccg atatgggtta aaacttcgct acgatcgaca aaagaaattc cgctacgaac
      781 aaacaggtta gtcatatacc gtccttattc cgcacagccg tagcgatagg cgcgcaaaac
      841 atcataaatt ttatccataa tcagctgatg cggtattgct gataattccc cggactgaat
      901 gcgttcaaat tgtttgggaa gatactgact aatcatgccc agtgggatgt ccacgccttg
      961 cagattcacc atcatcgttt caacgctatt tttaatccga ctatgcggcc agtaataacg
     1021 aatacgatcc gacaggctgt aacgaatatc cagtaatgaa tcgttaaaac ccgtgcgata
     1081 atattttttc cagtattgcg gttcgtcgag catcacttct tcaattaccg ccaggcaacc
     1141 gctgcgattt tcgggggcga taagttcctg ctcaatttgc gccagcgcaa atatcgcctc
     1201 acgtaaagca aaggttaatg cgggaccgac tttcaatatt gcaaagtgat cgcggactaa
     1261 ttcccaataa gccgtccggg tctggtaatc ggtagaatgt gcttcataaa ccattcgggt
     1321 attttctatc cattgcgcca gcgcctgcgc ttcctgcggc tgataatgga taatattgct
     1381 gtgatcaaat tccacacccg gctgcaccac gatggcaatc acgcgtgtta atgcctctgt
     1441 cagcccacgg gcaataaagg ccttttgatg cgtacgtaaa gtattggcgg catcttcaac
     1501 atgggtgatg tgtactgact gaatggcgct ggcctcaccg cccggaaccg gaacttcggt
     1561 gccaatgaca tagctcagtt gctcacgctg gcaatctgtc gccacacttt ccgcagcaaa
     1621 gcaaagcaca gcagctcgtt ccgcaaccgt ttctggtgct aacggtatgg gatcccccgc
     1681 gcaggacatt gacgcatcaa gatgaatttt actgaagcca gcacgaacat atgcctttac
     1741 cagctcgacg gatttttcca tcgccgcatc cacattttct tgctgccagc agtttggccc
     1801 cagatggtca ccgccgagaa taatacgctc gcgtgcaaat ccgactttat cggcaatcgc
     1861 aaaaacaaat tcgcgaaagt ctgccggtgt cattccggta taaccgccaa attgattgac
     1921 ctggtttgac gttgcttcaa tcagcacttt gcgcgtgctg ttgcgatcaa atgccagcgc
     1981 cgcttcgata accaacggat gggcagaaca gactgaacat atgccgatat gttcaccagc
     2041 tttatgccgg gcaattaacg ttttcatgtt ttttccttgt taaatggcag gtgcgttatg
     2101 ccctgccctc gcagccacaa tcggcaatca ctttgctcac cacatcgcgc attgcggatt
     2161 tagccgactg caaataatcc cggggatcgg tcgcttcggg gtgctcggtc aggtaatttt
     2221 ttaacgcctg cgagaaggca tttttcagct ccgttgcaac gttgattttg catatcccca
     2281 gtttgatggt ttgctgaata tccttagtcg ataaccctga cgcgccatgc agcaccagcg
     2341 gtaagttcac ccactggcga atgttctcca gtcgagaaaa atcaagcgcc ggtgcgctgg
     2401 catacatacc atgagccgtg ccgatggcga ccgccaggga atcaattccg gttgcctcgg
     2461 caaattcacg cgcctgagcg gggttggtat aaaaagcatc ggcttcattg acttgcacat
     2521 catcttcctg gccgccaagt tgccccagct ccgcttcgac gctgacatca aagcgatggc
     2581 aaaaatccac cacctctttg acccgtgaaa tattttgcgc aaaaggcaaa tgcgaggcgt
     2641 caatcatgac tgagcgcacg ccagaacgaa ccttctgagc gatatcgtca aatttcgtgt
     2701 gatggtcgag atgaatcgcc agtggatggt gataatgctt cgccatcgcg ctgaccagcg
     2761 ccaacagatt ttctgtacca gcatgagtaa atgtgccagg cgttccggcg atgatgaccg
     2821 gcgcatgcag gttggcagcg gtttctacca ccacttgcat cgtttcgaga ttgtgaatat
     2881 tgaatgccgg aaccgcgtaa ccgccgcgct gcgcgttgtt cagcatctgc tttgtcgata
     2941 ccacgtacat tttcacatcc tgtcgattgc tttcgatttc aaaacataat gaaatcattt
     3001 gtttttaaat gtcgagataa cgatcacaaa aacgacaata tgaaaattaa tagaggaatg
     3061 aaaggcaaaa aaaacggcct cccggaggga agccgcaaca aagtgcgcgt ggttttatgc
     3121 aggatgcggc gtaaacatat gtggcagcgc tgcgtaggca tgataagacg cttcagcgtc
     3181 gcatcaggca taggttgccg gatgcggcgt aaacgcctta tccgtcctac ggggtagtgc
     3241 cgttcgtatc aggcgatagt aattttgcta tcgagataaa cgtcctgcac ggcgttaatc
     3301 agtttcacgc cgtcagccat cgatttcttg aacgccttac gtccaagaat cagccccatt
     3361 ccgccagcgc gtttgttgat aaccgcagta cgcactgcat cactgaggtc agtttcaccg
     3421 cctgcagcac caccggagtt tatcaacccg gcccggccca tatagcagtt agctaactga
     3481 taacgcacca gatcaatcgg gttttcgctg gttaacttgc tgtacacgcg atcgtcggta
     3541 taaccgtaat taattgcttt atagccgccg ttattttccg ccattttttg tttgacgata
     3601 tctgcaccta tggttgccgc cagatggttc gcctgaccag tcaggtcggc ggagacatgg
     3661 taatcaacgc catctttctt aaaggcggag ttacgcaaat aggcccacag cactgtcacc
     3721 atgcccagct cgtgcgcacg ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac
     3781 tcttccgagc caaaatagat agtcgcacca accgccaccg cgcccatgtt gaaggcctgc
     3841 tccacgctgg catacagcgt ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga
     3901 ttaagtttta cgagaaatgg aatgcgatgc gcatagcgcc gcgataccga cgccaacacg
     3961 ccgtaagttg atgccacaca gttacagccc gcttcgatcg ccagttcaac aatgtttttc
     4021 gggtcaaagt agagcgggtt agcagcaaat gaagctccgg cagagtgctc aacgccctgg
     4081 tcaaccggca gaatagaaag atatcctgtg ccagccagac gcccggtgtt gtacaacgtc
     4141 tgcatattac gtaacaccgc tggcgggcga ttattgtcaa tcatcacgcg gtctacgtag
     4201 tcatgtccgg ggagataaag ctggtcagaa ggaatagtca tacaacggtg ctgtaaaagg
     4261 ttgtcggcgt ctttgccaag caactgcgca atatctgtca ttactatgct cccgtaaatt
     4321 ccgattggat atcggctatg gattgtcctg gcccgccttt tgcgggcaat cataatcctg
     4381 gtcgttacgg gcgagttttt ccatcttttg gatgtttttt cagcgttttc tgttggctcg
     4441 attcatcaga aaaaatgtta gcgcggtcaa gttttcaccg caaaggtatt taaaaggtat
     4501 tattaagtgg tattgtcatc gcgtacctta cattacctgt catgaaggaa ttaaaagatg
     4561 aaaacaacaa caaagctgtc gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg
     4621 tttgtgccat tgtggctgtg gttaagtaaa agcggtttta gtgccggaga aattggctgg
     4681 tcgtatgcct gcacagccat tgcggcgatc ctgtcgccaa ttctggttgg ctccatcact
     4741 gaccgctttt tctcggcgca gaaagtgctg gcggtattga tgttcactgg tgcggcgctg
     4801 atgtatttcg ctgcgcaaca gaccactttt gccgggttct tcccgttact gctggcctac
     4861 tcgctaacct atatgccgac cattgcgctg actaacagca tcgcttttgc caacgtgccg
     4921 gatgtggagc gtgatttccc gcgcattcgt gtgatgggca ctatcggctg gattgcctct
     4981 ggtctggcat gtggtttctt gccgcaaatg ctggggtatg ccgatatctc accgactaac
     5041 atcccgctgc tgattaccgc cggaagttct gctctgctcg gtgtgtttgc gtttttcctg
     5101 cccgacacgc cgccaaaaag caccggcaaa atggacatta aagtcatgct cggcctggat
     5161 gcgctgatcc tgctgcgcga taaaaacttc ctcgtctttt tcttctgttc attcctgttt
     5221 gcgatgccac tggcgttcta ttacatcttt gccaacggtt atctgaccga agttggcatg
     5281 aaaaacgcca ccggctggat gacgctcggc cagttctctg aaatattctt tatgctggca
     5341 ttgccgtttt tcaccaaacg ctttggtatc aaaaaggtat tattgcttgg tctggtcacc
     5401 gctgcgatcc gctatggctt ctttatttac ggtagtgcgg atgaatattt cacctacgcg
     5461 ttactgttcc tcggcatttt gctgcacggc gtaagttacg atttttacta cgttaccgct
     5521 tacatctatg tcgataaaaa agcccccgtg catatgcgta ccgctgcgca gggactgatc
     5581 acgctctgct gccagggctt cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg
     5641 gaaaagatgt tcgcttatca ggaaccggta aacggactga ctttcaactg gtccgggatg
     5701 tggacttttg gcgcggtgat gattgccatt atcgccgtgc tgttcatgat ctttttccgc
     5761 gaatccgaca acgaaattac ggctatcaag gtcgatgatc gcgatattgc gttgacacaa
     5821 ggggaagtta aatgaaaaca gaacgtattc tcggtgctct ttatgggcag gcgttagggg
     5881 atgcgatggg gatgccctcc gagctttggc cacgcagccg cgttaaagca cactttggct
     5941 ggattgaccg ttttctgcct ggaccaaagg agaacaacgc ggcctgttat tttaaccgcg
     6001 ccgaattcac cgacgatacc tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg
     6061 aaggcaagat cgacccgaat ctgattgggc gtaatattct cgactgggcg ctgcgtttcg
     6121 acgcctttaa caaaaacgta ctaggtccga cctcgaagat tgcgcttaac gccattcgcg
     6181 acggtaaacc cgttgctgaa ctggaaaaca acggcgtgac caacggcgca gcgatgcgcg
     6241 tctcgccatt aggttgtttg cttccggcgc gtgatgttga ttcctttatt aatgatgtgg
     6301 cgctggcgtc gagcccgacc cataaatccg atctggcggt tgcgggcgcg gtagtcatcg
     6361 catgggcgat ttctcgtgct attgacggag aaagctggtc agcgattgtt gattcattgc
     6421 cttcaattgc gcgacatgca cagcaaaaac gcaccaccac gttcagcgcc tcactggcag
     6481 cgcggctgga gattgcgctg aaaattgtgc gcaatgccga cggcactgaa tccgccagcg
     6541 aacagcttta ccaggtcgtt ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca
     6601 ttgcgctggt tgaactggca caaaccgacc cgaatcgctg cgccgtcctg tgcgctaacc
     6661 ttggcggcga tacagacacc atcggtgcta tggcgacggc aatctgcggc gcgttgcatg
     6721 gcattaacgc tatcgatcct gcgttaaagg cggagttgga tgcggtaaat cagcttgatt
     6781 tcaaccgcta tgccacagcg ctggcgaaat atcgtcaaca acgggaggcg gcatgagcgg
     6841 cgctcgatta cacacgctgc tgcctgaatt aaccacgcgt cagccggtta tggtgattgg
     6901 cgcggcagtc attgatgtga tcgccgacgc ttatgccctc ccctggcgtg ggtgcgatat
     6961 cgaactgaaa cagcagagcg ttaacgttgg cggctgcgca ctgaatattg ccgtggcgtt
     7021 aaagcgcctc ggcatcgaag cgggtaacgc cttgccgctc ggtcagggcg tgtgggcgga
     7081 gattattcgc aaccggatgg caaaagaggg cttaatcagc ctgatcgata acgccgaagg
     7141 tgataacggc tggtgtctgg cgctggttga gccggatggc gaacgcactt ttatgtcatt
     7201 cagcggtgtg gaaaatcagt ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg
     7261 cagcctgctc tatttttccg gttatcaact ggcctcgccc tgcggcgaat tattagtgga
     7321 atggctggaa aagctgcaag acgtgacgcc gtttatcgat tttggcccac gtattggcga
     7381 tatcccggag gcattactgg cgcggatcat ggcctgtcga cctttagtgt cgctcaatcg
     7441 tcaggaggct gagattgccg ccgaacgttt tgctttatcc gcagagataa caacacttgg
     7501 caagcaatgg caggagaaat ttgccacgcc gttgatcatt cgtctcgata aagagggcgc
     7561 atggtatttc agcaacgacg cttctggctg cattccggca tttcccacgc aagttgtaga
     7621 caccattggg gcgggcgaca gtcatgcagg tggcgtactt gccgggttgg cctctggtct
     7681 gccactggcg gatgccgtat tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg
     7741 gggcggtgat tgtgcgccaa cgcgcgagga actactcctc gcacacaaaa acgtatagat
     7801 cgctgcgaca gtggctaatg ctgtactcaa taggccgttg ctgttggtca agcgccactt
     7861 gcttgatcac cagcaccggt attttgctat ctaactgaat atgtgactga aactcggcat
     7921 ctggcatccg ggcgctaacg cgggaacgtg tacgttgtgg gtaaatgtgc tggctgcgga
     7981 aatagtcata aagagaaatt ccgatggcat caacatcatg aattaaatgc gccggaaccc
     8041 acgattcctc aatcgatacc gcttcttcat cgacatagcg aatacgctta agcaaaaaga
     8101 catcacttcc cgcttcgacc gccagctgct gcgcgacttc ttcgggacat ttcacgacac
     8161 gcttgttaac ccatagcgta tcgggctttt tcccgcgcaa taccacctgc tgagaaaaac
     8221 ctcgcgcttc tttcaacgag tattcgaaga tgttgttgat ctgcgtgccg taaccgcgcg
     8281 aacgcgtcac cacaccctct tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgtg
     8341 aaacgccggt taactgactt aaatcacgct caccgggcaa aatattgcca tgctccagca
     8401 ccccgctgcg cacggcattt tttaccgttt cggcaaactt aatataaagc ggcgtgttat
     8461 ccgccgctaa aatacgctcg ttcagttggg caatcagctg ggtatgcgct tgttccattt
     8521 gtctttttcc tgccagggag tctggtggga gtatatcact gcttcttatt taaggcgttt
     8581 ctttaatccc atcaacgaat gcctgcaact cctcctctgt gccattaaac acattaaaat
     8641 ccaccgaacc attaatgcca tctacctgcc cacggtcgga atgctgccag aaacgccagg
     8701 ccatgccgtc attgtccggg cgacgttgat agtagtgagc cacccaccac ggatattcat
     8761 tgaaatagcc cgccagattg gtgtgataaa aaacggctcc tgagtaaata accggctttt
     8821 ttcccgtact tttttcgacc atttttagcc actgacttac ccgcttgcgt aattcttttg
     8881 ccgataattt tccccgttct tctacgtcca gcacggcagg gaaatcgcct tgtgagaaat
     8941 ccaccgtttg cagaaataat cgcgcctgaa caggcgctgc taccgacggg gagaaataat
     9001 gatacgcccc gcgcagcagg ccattttcgc ggcttagttg ccagttacgc gaaaaatagg
     9061 ggtccaccag cttttcgcct tccgtcgcct tgataaaggc aaattgtaag cggataccgt
     9121 tatcgcgcat ttttgccacc cgctgccagt cgatccgctc ctgccagcgc gaaacatcta
     9181 ttccgtgaat ggtgtaactg gcaggaatat gaatagcgaa agatttaacc gggcgataac
     9241 cgtagaagtg gatgtagtct gtaatctgaa ctgctgtcga atagaaaaaa ttcaccgtct
     9301 gacgcggata aattgcaacg atggaaatta gcccaagagc gcataataga gcggtgagtt
     9361 tttttcgact ggtaattctt agctgcattg gtttcatccc tgaatgtcag tgccaaaggc
     9421 tgacaataac caaagcagct atagtacggt gctcaccacc aggcgtggaa gtggtgaacc
     9481 ggaccaatac cgtgaccaac ttccagcgtg tcggcctggg ctaacgccga tgaaagccag
     9541 ctttttgcct cctgtacggt gtcagcccag tttgtatggc gcgggcgtag tgcagccaac
     9601 gccgcagaga gtgtacaacc agtgccgtgg gtgtttttgg tcataatgcg cggtgcggta
     9661 aaccgttgtt caccctcgcg ggtaaacagc cagtccgggc tttgctcatc atccagatgg
     9721 ccacctttca ttagcactgc gccacagccc atcgccaaca gcgctcgccc ttgttccagc
     9781 atttcctgtt cggtgcgcgc gtgtggcgcg tcgagcaagg cagcagcttc gggcaagttt
     9841 ggcgttatta atgaaacctg tggcaataat cgactgcgta gcgtggcaac tgccgaaggt
     9901 gaaagcagtg ggtcgccact ttttgccagc ataacggtgt cgagtaccac gttttggatc
     9961 tgataacgtt gcaaccgttc tgccaccgct tcaacaatat cggtttccgc caacataccg
    10021 attttagtgg tatcgattcg cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa
    10081 tcaggctcaa tgcgatacac cgactgtacg ccacgggtat tttgcgccac cagtgcagta
    10141 ataactgagc aaccataagc gccaagtgcc gagaaggttt taagatcggc ctgaatcccc
    10201 gcaccaccac tcggatcagt accggcaatc gtcagagcgt taattcgttt catgcctgca
    10261 cctcctgcgt caattgccag agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct
    10321 cgcttctggc gactgcgcgt tctccggctt gtttcatcca gtgacaggca gatgcgacat
    10381 tttccagcat atcgcctggt aacgcacaac aggcagcgac aaccgccgat aatgcacagc
    10441 cagttcctac cactttggtc attaacggat cgccaccgtg aatgccaacg gcgcgatggc
    10501 catcggtaac ataatccacc tcgccagtga ccacgacgat tgcgccagtt tcccgtgcca
    10561 gtgtttgtgc agcgggtatc gcgttaactg cggcgtcagt ggtatccact ccccgtccac
    10621 cattagcaac gccagctaat gccatgattt ccgaagcatt accacgtatc gctgccggtt
    10681 taaaagataa aagttcatga caaaaatggc ggcgataatc gagcgcacca accgctactg
    10741 gatcaagcgt ccagggtgtt tgagagcttt ttgcttgctc aacggcagca cgcatcgcct
    10801 gtgcgcgtgg ctgcgtcagt gtgccaacgt taatcaacaa ggcactggcg atagccgcaa
    10861 actgactggc ctcttcggtt tcgataacca tcgctggcga tgcaccgagc gccagcaagg
    10921 tattggcagt aaaggtttgc accacatcgt tggtcatgca gtgcacaaga ggggaatgtt
    10981 ggtgaaaaag gtgtaacgtg tgcgcagatt gcgcggaact cagcaggtcg acttgcatag
    11041 tttgctcctg ccataacgtg aagaagcaat gacctggtgg tccgtgactt ccctacgctg
    11101 gcattatcca gatcaggtga tacgggtatt tctcagcctt cacgcagaag ggcaccccga
    11161 gtcgtttggt tgaatctcta tattttgtgc cagcttaggg gaagaggctt gcagatgcaa
    11221 gctcgcctcc attcattacg tcttcactgt gtaccccata tcagtaatga aaaagaaaca
    11281 attcacaccc atcattcagg tggtaaaaac tgtagactat cttaattagc taatttatat
    11341 tatttatcta aatttgatag gtgagataaa aatgacagaa acccagatct acgagaacat
    11401 taagcaagcc attagcagcg caccacgaaa tagccaaacg atggaaatgc acctccagat
    11461 gatcaaatac gctgaccatc tgaaaaacgt cacggcaaaa gagttttgcg aaggtgtagg
    11521 acttaaagcc agctttgcta cagagttcag caaaatgaga aatcttaccg agcgactcaa
    11581 agccgccgga cttgatacca cgaagttata acaaacgaaa tcatcaatat attactttcg
    11641 ttttccaggg ttccctgtat atgtacctat gatcataaaa accagttacc accacgtagc
    11701 tgataccata atattttcat ttttacttta aataaataga taaatttaga gagtggaata
    11761 attaaggtct attttctcta gctttttgag attgatagtt gagaatgata attctttcat
    11821 ctcaataaca ccgaagtttg atagctgttt aagccacagt tgaagaccag catcagagca
    11881 acctgctcga tatgttacat cactacattt tattagtgtt gatttcctct tatagagata
    11941 aataaggact tcatagatgc gttttccggt gtcattggat acgcgctata cccccattct
    12001 tggctaaatc ttggttcata atcatacttt aattaaaatg gatctaaaaa ttcatcatag
    12061 atggctggat ttttcataga aaaat
//
LOCUS       DBFIFS010000135        11885 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.135, whole genome shotgun sequence.
ACCESSION   DBFIFS010000135 DBFIFS010000000
VERSION     DBFIFS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.135"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            232..525
                     /locus_tag="N7A07_003226"
                     /pseudo
     CDS             232..525
                     /locus_tag="N7A07_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(583..1014)
                     /gene="tnpA"
                     /locus_tag="N7A07_003227"
     CDS             complement(583..1014)
                     /gene="tnpA"
                     /locus_tag="N7A07_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HGU2477840.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            1022..2230
                     /locus_tag="N7A07_003228"
     CDS             1022..2230
                     /locus_tag="N7A07_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU2477841.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2274..2381
                     /locus_tag="N7A07_003229"
                     /pseudo
     CDS             2274..2381
                     /locus_tag="N7A07_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2365..3003)
                     /gene="ytfB"
                     /locus_tag="N7A07_003230"
     CDS             complement(2365..3003)
                     /gene="ytfB"
                     /locus_tag="N7A07_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU2477842.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3221..3841
                     /gene="fklB"
                     /locus_tag="N7A07_003231"
     CDS             3221..3841
                     /gene="fklB"
                     /locus_tag="N7A07_003231"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2477843.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4150..5562
                     /gene="cycA"
                     /locus_tag="N7A07_003232"
                     /gene_synonym="dagA"
     CDS             4150..5562
                     /gene="cycA"
                     /locus_tag="N7A07_003232"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU2477844.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5607..6269)
                     /gene="ytfE"
                     /locus_tag="N7A07_003233"
     CDS             complement(5607..6269)
                     /gene="ytfE"
                     /locus_tag="N7A07_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU2477845.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6377..7342)
                     /gene="ytfF"
                     /locus_tag="N7A07_003234"
     CDS             complement(6377..7342)
                     /gene="ytfF"
                     /locus_tag="N7A07_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2477846.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7450..8310)
                     /gene="qorB"
                     /locus_tag="N7A07_003235"
     CDS             complement(7450..8310)
                     /gene="qorB"
                     /locus_tag="N7A07_003235"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU2477847.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8399..8779
                     /gene="ytfH"
                     /locus_tag="N7A07_003236"
     CDS             8399..8779
                     /gene="ytfH"
                     /locus_tag="N7A07_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU2477848.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8897..10840)
                     /gene="cpdB"
                     /locus_tag="N7A07_003237"
     CDS             complement(8897..10840)
                     /gene="cpdB"
                     /locus_tag="N7A07_003237"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU2477849.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11030..11770
                     /gene="cysQ"
                     /locus_tag="N7A07_003238"
     CDS             11030..11770
                     /gene="cysQ"
                     /locus_tag="N7A07_003238"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU2477850.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2832 a         2954 c         3086 g         3013 t
ORIGIN      
        1 tgaatgttca cagaaaaaaa tcgatattaa taaaaataag tttgttttct gaagtggtga
       61 aaatagcgga tggtgaaaga tattaatatc aaaaatcgct cattatggat ataacgatgc
      121 acccggggag aaaatgactt ccacgcctta caccgtacat tcttgttgtg cggtatgtaa
      181 ttattctacg ctgtcggtat cggtgtattc gttaatcagc aggttaacca gccacaaaaa
      241 gccattgaga aaattattga ttttacatgg gattattata ttgctaaccc ctggttttta
      301 aaaattgtgc atagtgaaaa tcaaagtaaa ggtgtgcatt atgcaaaatc acagcgtctc
      361 ttagaaatca accatgcgca cttgcagtta atggaatcct tattggatga agggaagaaa
      421 tacaatattt ttaagccaga tatcgaccca ttgcaggtga atattaatat tgctgcgctt
      481 gggggatatt atttgatcaa ccagcatacg cttggcctgg tttatccggt gcgccgtaaa
      541 accccgtcct tcagggcggg gatatacggc gcggttttcc acctaaccag gtgtttgctg
      601 ttgttcaatg tattgacgga tggcggatat tggcgcacct ccgcagctac tggcaaaata
      661 gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg gtcgatctcg
      721 tcgcagtaac ctacccgata cgcctttgag gctgtttacc agactggata tcgccagttt
      781 gggaggatag ttaattaaca aatggacgtg atctggttcg ccatccattt caaccagttc
      841 agcttcaaaa tcagcacata cctttgaaaa gtaagtgcgt agtttttctg tcgcgtcatg
      901 gtcaaaaatc tggcgtcggt atctggtgac aaagaccagg tgaacatgca tcaggaaaac
      961 acaatgcctt ccacgccgga tatcggtttc ttttttcaca gaccaaagta tcatttggcc
     1021 tgtgaaacga ctacaggcat ttaaattcca gttaagaccc ggtggtcaac aggagcgtga
     1081 aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg cgcttcagaa
     1141 tgagaatcat gaggccggta ataaatacat cccttacggg aaaatggctt cctggctggt
     1201 tgagtggaaa aatgccactg aaacgcaatg gcttaaagat gccccgtcac agccattgca
     1261 acagtcactg aaagaccttg agcgggctta caaaaacttc ttccggaagc gggctgcttt
     1321 tccccgattc aaaaagcggg gacagaatga tgcattccgc tacccgcagg gcgttaagct
     1381 cgatcaggaa aacagccgta tttttctgcc gaaacttggc tggatgcgct accggaatag
     1441 ccgtcaggtc acgggtgttg tgaaaaatgt cactgccagc cagtcctgcg gtaagtggta
     1501 catcagtatt cagactgaaa atgaagtatc aactccggtt cacccttcag cattaatggt
     1561 cggactggat gctggcgtgg ctaaactcgc cacgctgtca gatggcacag tctttggacc
     1621 tgtaaacagt tttcagaaaa accagaagac gctggcgaga cttcagcgac agttaagccg
     1681 caaggtcaaa ttcagcaaca actggcagaa gcagaaacgc aaaatacagc gactgcattc
     1741 ctgtatcgca aatatccgca gggactacct tcacaaagtc acaacgaccg tcagcaaaaa
     1801 ccacgcaatg atagtcattg aggatttgaa ggtcagcaac atgtcaaagt cagcagcggg
     1861 tacggtcagc cagccggggc gcaatgtccg ggcaaaatca ggtttaaacc gttcgatact
     1921 ggatcagggc tggtatgaaa tgcgccgcca gcttgagtac aagcagctct ggcgtggcgg
     1981 tcaggtgctt gctgttccgc cagcgtacac aagccagcgt tgcgcgtgct gtggtcatac
     2041 agcgaaagaa aatcgcctgt cacaaagtaa attcagatgc caggcatgtg gatatacagc
     2101 gaacgctgat gtaaatggcg ctcgtaacat tttagcggcg gggcacgccg ttcttgcctg
     2161 tggggagatg gtgcagtcag gccgcccgtt gaagcaggaa cccaccgaaa tgattcaggc
     2221 gacagcctga acgtagcagg gatccacgtc cttcagggct tggaggatgt caacacatca
     2281 gtatggtttc accccaggca ctggaagcca gacgtaaggt catcaaagag acaatcctta
     2341 gctggctttt ggttgaccct tcatctaccg cgcacgaata aaactgccat ccggctggcg
     2401 ggtgaacagg acctgttgat tattccccgt atcaatggtt aagcccgtca ccacgccgct
     2461 ggcgttctgg cggattttca ccatctgccc gttttgcaaa ttactcagcg gcttacccgc
     2521 gccttctacc tgcgccattg catagacatc ggtagcgggc agtccgtggt cgcggaacag
     2581 ctgcgccatc gttttaccgg gttcgacacg gtaagagcgc cattgattat caattccgct
     2641 atctggctgg aatggttgcg tttgggtagt tttcggctgt tcttctggct ggccttcctg
     2701 tataggttct ggagcgaccg gagccacctg atcaggataa ttttgtgggg tgactaattg
     2761 cgcccgcagc tgttcttctg tcggcggttg tgattgcgac tgaatatcta actgcgcttc
     2821 acgcgtcacc accggcgcgt tgggcgtatc atcagatgga agcagaaatc cgaccaccaa
     2881 tacaatggcg gcaataatga tcccccgacg atgcatcggc ggcagcgggt ccatgaagcg
     2941 aaaattgtcc ggtgcgtgcc agactttctc cagggttggt tttagttcaa agcgcccggg
     3001 catggtttcc tcctgctccg cgtcttgttc ctcaatcata gcctatgaat aagctaacgc
     3061 tatgatgtcc gtggtaaacc cgcctttatt atattcatac gggatattgc tattgtttct
     3121 ttttccctgg gatttgtcat cattcccgcg acaaagtttt acccaaagaa gtgtggctga
     3181 tatgctgccc gctactttat accctaagaa aggaaatacg atgaccaccc caacttttga
     3241 caccatcgaa gcgcaagcaa gctacggcat tggtttgcag gtagggcaac aactgagtga
     3301 atctggcctg gaagggctgc tgccagaagc actggttgca ggtattgccg atgcgctgga
     3361 aggcaaacat ccggctgttc cggttgatgt ggtgcatcgc gcgctgcgtg aaatccacga
     3421 gcgcgccgat gccgttcgtc gtcagcgttt ccaggcgatg gctgctgaag gtgtgaaata
     3481 cctggaagaa aacgccaaaa aagaaggtgt gaatagcacc gaatctggcc tgcaattccg
     3541 cgtgatcaac cagggtgaag gcgcaattcc ggcacgtacc gaccgcgttc gtgttcatta
     3601 caccggtaaa ctgatcgacg gcaccgtgtt tgacagctcc gttgctcgtg gtgaacccgc
     3661 tgaatttccg gttaatggcg tgatccctgg ctggattgaa gcactgactc tgatgccggt
     3721 aggttctaaa tgggaactga ctatcccgca ggaactggca tatggcgagc gcggcgcagg
     3781 cgcatccatc cctccgttca gcaccctggt gtttgaagtc gaactgctgg aaatcctcta
     3841 agcagcgcat tctgttcccc tcgaaccaga ggggagcagg cattcagcaa taaacccttc
     3901 agtttgccaa acggcgctat tttgtgttgc aaagaccccg taagcgtgta tttttgtgag
     3961 ctgtttcgcg ttatcaccgt gatatgacac tcactttaaa cataaaatta acattagatc
     4021 taaatcttag tattcatccc gcgtattgtt acctaatatc gatgagtccc gatacagatt
     4081 cgtcgtatca tagactgact aaaggccgta gagcctgaac aacacagaca ggtacaggaa
     4141 gaaaaaaaca tggtagatca ggtaaaagtc gttgccgatg atcaggctcc ggctgaacag
     4201 tcgctacggc gcaatctcac aaaccgacat attcagctta ttgccattgg tggtgccatt
     4261 ggtacggggt tgtttatggg gtctggcaaa acgattagcc ttgccgggcc gtcgatcatt
     4321 ttcgtttata tgatcattgg ttttatgctc tttttcgtga tgcgggcaat gggggaattg
     4381 ctgctttcga atctggaata caaatctttt agtgacttcg cttccgattt actcgggccg
     4441 tgggcaggat atttcaccgg ctggacttac tggttctgct gggttgtaac cggtatggca
     4501 gacgtggtgg cgatcacggc ttatgctcag ttctggttcc ccgatctctc cgactgggtc
     4561 gcctcgctgg cggtgatagt gctgctgctg acgcttaacc tcgccactgt gaaaatgttc
     4621 ggtgagatgg agttctggtt tgcgatgatc aaaatcgtcg ccattgtgtc gctgattgtc
     4681 gtcggcctgg tcatggtggc gatgcacttt cagtcaccga ctggtgtgga agcgtcattc
     4741 gcgcatttgt ggaatgacgg cggctggttc ccgaaaggtt taagtggctt ctttgccgga
     4801 ttccagatag cggttttcgc tttcgtgggg attgagctgg taggtacaac agctgcggaa
     4861 accaaagatc cagagaaatc actgccacgc gcgattaact ccattccgat ccgtatcatt
     4921 atgttctacg tcttcgcgct gattgttatt atgtccgtga cgccgtggag ttcggtagtc
     4981 ccggagaaaa gcccgtttgt tgaactgttt gtgttggtag gtttgcctgc ggctgccagc
     5041 gtgatcaact ttgtggtgct gacctctgcg gcgtcttccg ctaacagtgg cgtcttctct
     5101 accagccgta tgctgtttgg tctggcgcag gaaggtgtgg caccgaaagc gttcgctaaa
     5161 ctttctaagc gcgcagtacc cgcgaaaggg ttgactttct cgtgtatctg tctgctcggc
     5221 ggcgtggtga tgttgtatgt gaatcctagc gtgattggcg cgttcacgat gattacaacc
     5281 gtttccgcga ttctgtttat gttcgtctgg acgattatcc tttgctcgta ccttgtgtat
     5341 cgcaaacagc gtcctcatct acatgagaag tcgatctaca agatgccgct cggcaagctg
     5401 atgtgctggg tatgtatggc gttctttgtg ttcgtggtcg tgttgctgac actggaagat
     5461 gacactcgcc aggcgctgct ggttaccccg ctgtggttta tcgcgctggg gctgggctgg
     5521 ttgtttattg gtaagaagcg ggctgctgaa ctgcggaaat aaccgcatta tcatgctgga
     5581 tggcgcaatg ccatccagct tttagatcac tcacccgcca gcgcccttgg gaacagtaca
     5641 ttgttttcca ggctgatgtg atccatcagg tcatcaatca gttcattaat gccgttatac
     5701 atcgctttcc aggtggtgca ggcttccggc ggcggtgtga cgttattggt attgtgtttg
     5761 atcacttcca gcagttcacc cgcttcatcg tgctcgcttt ccattacgct gattggcccc
     5821 attgcctggc tgcccatgcc ttgtttgatc atcgggaaga ggatctgctc ttctttcatc
     5881 atgtggctgg aaagctcttc atgcagcatg gtcaggtatt ttgtcagccc ttttggcacg
     5941 ctcggtttgt cggcgtgaac gcgctcgact ttagtcgctt gcagaatcag ctccggcagt
     6001 tgctcgcggt gacgagcgtg gtagcgcacg atgatatggt cgatgatttc tgccagcggg
     6061 gcgctacgcc agtctttctc aatcggttgt tcagcaagct ttgccagctc agcttcaatg
     6121 acctcaacat ccagttcttt acgtgccgcc gcacgcgcca gcgtttgctt accgccgcag
     6181 cagtaatcca tatcatattt acgaaacaga gccgaagcgc gaggaataga gagcgccagt
     6241 tcacctaaag gttggtcgcg ataagccata gctgatacct cattcttaat aagataagat
     6301 gtattttaaa tgcatcttta aggcaaaagc tataaccctt acgtagcaga ggcttattta
     6361 actcactgcc agcgtgtcaa ctttctgaga cagattcagt taaaggtttt tctggttttg
     6421 ctctaaccgc gatgaccacg ccaatcacta acagcgcgat accgctcagc gtcattagcg
     6481 gcggcatttg ctggcggagt aaaaaggtgt acagcaaacc tgccagcgtt tcgaaaacaa
     6541 tcagcggccc gagaatcact gtcggtaatc gctggctggc gacgttccag cagagtgcgc
     6601 caacccatga gcaaagcacg gctatcgcaa tcatcagact aataaacacc agcggacggg
     6661 ggccaaaggg tagggagaag tccggcgttt gcgtattcag ccagtaacaa gcgacgagat
     6721 agccgataag tgaaaccggc agtgtgacca gcgcctgcgc tgttgcccac atcatcggat
     6781 gtttgtcggg attttcccgc agccagcggg cgttgcgcag ggcataccac gcccagcaga
     6841 ccacggaaac taacgccagc acgatgccag aggtataacg tgcccagtca aaattgggga
     6901 gtccgtggtt taactcagca atattcacac acgccaggcc gatgccaata caaatcagtg
     6961 ccggggcgag ttttccccac gcgagtttgc cgtcgcgctg gctataaagc agattggcga
     7021 agacgggaat caccaccggc agggtgccga taatcatcgt ggaaacaggc gcaccagtac
     7081 gttgaatggc actggcaagg cagaagtaat agatgaggtt gcccatcata gtgagcatca
     7141 aggcggtaag ccagtcgcga cgcgccaact gacgcagacg cacgcgtccc agccaggcaa
     7201 tgggcagcgc aattaaccct aacgccagat aacgccccat cgactgcaac atcgccgggt
     7261 attccggcac gatcaacggc ccgacaaaaa taagccccca catcaaccct gctaacaggg
     7321 cgtacagcac gccgctaatc attactggca tccattgatc tgtcagaaga aagttcagga
     7381 agcagacatg attgctcccg gcatcccgtc attatcagag aggatgcggg aggatgccac
     7441 tttaattaac tagttattaa cattaaaaag atggcttacg ctttcggcta acgttgtcgt
     7501 tgggtggcca atcaatttgc taagtgtttt gctgtcatca aacagaccgc ctttcgatgc
     7561 gccaacgtca gaatccgcca gcatatccgc cagtccgtcg ggcagtccga cgcttttcag
     7621 tgccgcggcg aaatcggctt cgctcagatt ttgataggta acctgtttgc cgctctgttt
     7681 ggtcagctct gccgctaact gtgtcaacgt ccaggcacta tcgcccgcca gttcgtaaac
     7741 cttgccttcg tgaccggctt cgctaatcac gcgtgccgca gctgccgcat aatctgcccg
     7801 cgttgctgag gcgattttgc catcgcccgc cgcaccgata aatacgccgt gttccagtgc
     7861 tgccggggcg ctggcgaggt agttttcggt gtaccagccg ttgcgcagta gggtgtaaac
     7921 gatgccagaa tcagccagca ttttctccgt ctcgatgtgc tcatcggcga ggccgagcgg
     7981 agatttatcc gcgtgaagca ggctggtata agcgataaat ttcacgccag ccgtctttgc
     8041 ggcattaata acattacgat gctgcggggc acgttgaccc acttcgctgg aagagatcag
     8101 cagtagtttt tccactccct gaagtgcaga tgtcagtgcg gcttcatcgc cgtagtcagc
     8161 ctgacgcacg gtaatgcctt gtgctgtcag tgcctgggct tttgccggat tacgaacgat
     8221 agccactatt tggctggcag gaaccgtttt catcaaggat tcaataacat actgaccaag
     8281 ttggccagtg gcaccagtaa tagcgatcat gggaagtctc catcgttttg tcttgtgttt
     8341 aaatacgcta acacctaaac ttacttttag taagtacgca caaaaaggta agtatgaaat
     8401 gagtcaggtt agcctgtcgc aacaactgaa agagggtaat ctctttgcgg aacagtgccc
     8461 gtcgcgcgag gtgttgaaac acgtcaccag ccgttggggg gtgttgattc tggtggcgct
     8521 acgcgaaggt actcatcgct ttagcgacct gcggcgcaaa atcggcgggg tgagcgaaaa
     8581 gatgcttgcg cagtcgttac aggcgttaga acaggatggt tttcttaacc gtatcgcgta
     8641 tccggtggtg ccgccgcatg ttgaatacag cctcacgccg ctgggcgagc aggtgagcga
     8701 aaaggttgct gcactggcgg actggattga gttgaatttg cccgaggtgt tggcggtgcg
     8761 ggatgaacgt gcggcataac tgccggatgc gcggcctacg gtgtatgact tgtagggcgg
     8821 ataagatgcg ccagcatcgc atccgccaat aaatgccgga tgcgattgat gatttatagg
     8881 ccgaaaaaga agtgttttac ttactcaaat ccacctgata aatcgcaaac ccgatatcat
     8941 cggtcgcgac tttattcatc ggatactgcc ctttctcttt aataaacgct gcggctttat
     9001 ctgacgggga agtttcgaaa cggatatcca gtttcttatc ggcagctatc ggtgctaaac
     9061 gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca tcagcaatcc
     9121 acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg
     9181 tcccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg aacatagcgt
     9241 tcggatcaat cggcttgcca ttaaaggtca ggttcttaat cctttccgca ttggcattaa
     9301 tcatctggca ctcgccgtca taacgggcgg gctgggtaac atcaatctga taattcacac
     9361 catcaataac atcaaagtta taagtgcgga aaccatccca gttgatgagt gactgtggtt
     9421 tcgtgctgtt gggatcaatc tggttaaact gtccggcgga gcactccagc cactctttca
     9481 cctctttacc gctggctttc accacaatca gcgtattggg atagagataa agatcggcgg
     9541 cattacggaa ggtcaactgg cctttttcca cctccacata gcttgccggg tcatttttgc
     9601 gaccaccgac tttaaacggt gcggcagctg aaagcaccgg cagttttgcc agatccggat
     9661 cgccctgaat gtaatgctcg acatacgctt tttgcgcgtt gttcactact tgcacggtcg
     9721 gatcgtcctg caccagcgcc agatagctat acatattgtc ggcagattta ccgattggct
     9781 tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc agcttgctgt
     9841 cttccgccgc gagggatttt ttattggcga tgtcgtaaat cggccgtgct tccgctttcg
     9901 cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc accccaagat
     9961 gatcgcccca catgcccggc attaccgccg gaacaccatt cagcgtgcct ttggtgatat
    10021 cagccccttc gatatcagca aaatctttac ccgggaaaac ggcgtgagca tggccaaaca
    10081 taatggcgtt aacgcccgga atttcactga ggtaataaac tgagttttcc gccattactt
    10141 tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgacatcg gcacctttct
    10201 cgcgcatttc aggctcgtat ttgcgcacgg tttcggtaat atcattcacc gtcacttttc
    10261 cggataaatt agctttatcc cagcccatga tttgcggcgg cacgacgcca atatagccaa
    10321 tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtgtcttta attaaatacg
    10381 gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca taagggaatt
    10441 tcgctcctgc cagcgcattt ttcaggtaat ccagaccgta gttaaactcg tggttgccaa
    10501 gcgttccgac ggtatagtcc agcgtattta atgccttata gaccgggtga atatcacctg
    10561 cttttaatcc tttcgccgac atgtaatcgg ccagcggact cccctgaatc aaatcgccgt
    10621 tatcgaccag tacgctgttt ttcacttcat tgcgggcatt gttaatcagg cttgccgtac
    10681 gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc
    10741 tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc acactggcgg
    10801 caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc cttttatcat
    10861 cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg aagaatgcca
    10921 caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt aatgataggg
    10981 tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa tgttagatca
    11041 agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg acggggcgaa
    11101 accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg atattgccgc
    11161 tcacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg tcctttctga
    11221 agaagatcct cccggttggg aagtccgtca gcactggcag cgttactggc tggtagaccc
    11281 gctggatggt acgaaagagt ttattaaacg taatggcgaa ttcaccgtta acattgcgct
    11341 cattgaccat ggcaaaccga ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta
    11401 cagcgcggca gaaggcaagg cctggaaaga agagtgcggc gtgcgcaagc agattcaggt
    11461 ccgcgatgcg cgcccgccgc tggtggtgat cagccgctct catgcggatg cggagctgaa
    11521 agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt cgctgaagtt
    11581 ctgcctggtg gcggaaggac aggcgcagct gtacccgcgt ttcggaccaa cgaatatttg
    11641 ggacaccgcc gctggacatg ctgtggctgc agctgccgga gcgcacgttc acgactggca
    11701 gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatccggggt tcagagtgtc
    11761 tatttactaa attcagatgg cagaaacagt gtatttcctg attctgccat cctgatttct
    11821 cccaacctaa aaagttataa ataaaaagag attgtattta aagtgcaaaa attcaattgc
    11881 taata
//
LOCUS       DBFIFS010000136        11844 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.136, whole genome shotgun sequence.
ACCESSION   DBFIFS010000136 DBFIFS010000000
VERSION     DBFIFS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.136"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            325..4308
                     /gene="ehaA"
                     /locus_tag="N7A07_003239"
     CDS             325..4308
                     /gene="ehaA"
                     /locus_tag="N7A07_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU2477851.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4450..5538
                     /locus_tag="N7A07_003240"
     CDS             4450..5538
                     /locus_tag="N7A07_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2477852.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5580..6511)
                     /locus_tag="N7A07_003241"
                     /pseudo
     CDS             complement(5580..6511)
                     /locus_tag="N7A07_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6603..7110)
                     /locus_tag="N7A07_003242"
                     /pseudo
     CDS             complement(6603..7110)
                     /locus_tag="N7A07_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7368..7973
                     /locus_tag="N7A07_003243"
     CDS             7368..7973
                     /locus_tag="N7A07_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU2477853.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8013..8876
                     /locus_tag="N7A07_003244"
     CDS             8013..8876
                     /locus_tag="N7A07_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2477854.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8866..10413
                     /gene="fdrA"
                     /locus_tag="N7A07_003245"
     CDS             8866..10413
                     /gene="fdrA"
                     /locus_tag="N7A07_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU2477855.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10413..11831
                     /locus_tag="N7A07_003246"
     CDS             10413..11831
                     /locus_tag="N7A07_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU2477856.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3127 a         2741 c         3196 g         2780 t
ORIGIN      
        1 cttttaccgg ctgtatatta aatgtatttc tcctgctttt gtttatattt tgagttgatt
       61 tgagttcccg gatacatata atgcgcaggt attagagact gggggggaat gtaatgataa
      121 agttaagagt gttaacttaa aataagtttt tagtagtaac ttgctatttt attaccctct
      181 gaactacata ctcagatatg tggttgccaa tgctatgcaa aatttttgaa tttttatatg
      241 cgatgaacag taaccggaat ggcatttaat gcgttgctgt ttatgcaatc ttggttttat
      301 ttggatgtat tgctggagat tgtgatgaat aaaatatatc ggctaaagtg gaacaggtcc
      361 cgtaactgtt ggagcgtctg ctcggagctg gggagcagag taaaaggaaa aaagtcccgg
      421 gctgttttaa ttagcgcgat aagtttatat tcatctctgg tattcgccga tgatgtcatc
      481 gtaaaccagg ataaaactat tgattttggc aaagagaacc agagcatcga ttaccgtatt
      541 acggtgacag acaatgccaa tctggtaatc aatgcgacag atacttcccg tccgcgtctg
      601 actctcgctt ctggtggtgg gttggatatt accggaggaa aggtaacaat caatggccca
      661 cttaactttt tgctgaaagg tacggggttc ctgaatgtct ccaatgctgg cagcgagtta
      721 tatgctgatg atttgtatga atcaaactca ggcatgagac acgatcgcgg ctattttaat
      781 gtctccaacg gcggcaaaat ccatgttaag ggcaccagcc gtctgaccta tttgcaggga
      841 aatgtcagtg gtgaaggtag ccaggtaaat tccgaaacct tctttatggg cgtttacggc
      901 agttacggtg gtaatcagta cctgtcagtt aataacggcg gtgaagttaa tgccaggaag
      961 caaattagcc tgggctatta tgatcaagtc tccgatacaa cacttgctgt ttcggaaggt
     1021 ggtaaaattt ctgcgcctac tattagttta agcaccaact ctgagttagc gttaggggca
     1081 caggaaggaa gcgcagcgaa ggcagcaggg attattgatg ccgaaaaaat tgagtttgtg
     1141 tgggcaaaga catccgagaa gaaaatcacc ttaaaccaca cggataaaga cgcgactatt
     1201 tccgcggata ttgtcagtgg cagcgagggc ctgggctata tcaatgcgct caatggcacg
     1261 acttacttaa ccggtgataa ctctgccttt agtggtaaag tcaaaattga gcaaaatggc
     1321 gctttaggga tcacccaaaa tataggtaca gcagagatca acaaccgcgg gaaattacac
     1381 ctgaaggctg acgatagcat gacctttgcc aataagatct ctggcaacgg tacaataagt
     1441 atcgacagtg ggacggtgga gttgaccggc aataactatg cattcagcgg atatattgat
     1501 gttgcttctg gtgctgtcgc tgttatttct gaagacaaga atatcggtcg tgcagagctg
     1561 gatgtcgatg gcaaattgca aattaatgcc aacaaagatt gggtatttga taacgatctt
     1621 gaaggtagag gcattgttga aataaacatg gggaatcacg aattctcctt cgatgagttt
     1681 gcttatacag actggttcca gggttcactg gcgttccaga acacgacatt taatctggaa
     1741 aagaatgctg agtttctgca gaaaggcggg atcactgcgg gtcagggaag cctggtaaca
     1801 gtgggtaagg gcgctcactc cattagcact ttgggattct ccggcggaac cgttgatttt
     1861 ggtgccctga cagcaggtgc acagatgaca gaagggacgg tcaacgttag taaaacgctg
     1921 gatttgcgcg gcgagggtgt gattcaggtt tctgacagtg acgttgtccg ctcagtatct
     1981 cgtgatattg actctgcgtt atcgctcact gaagtcgatg atggtaacag caccattaag
     2041 ttggttgatg cgcaaggtgc ggaagttctg ggcgatgcgg gcaatctgca attgcaggat
     2101 aaaaatgggc aaatcctctc cagcagcgcc caacgtgata ttcagcagaa tgggcaaaaa
     2161 gcggccgtcg gcacttacga ctatcgtctg acgagtgggg taaacaatga cggtctgtat
     2221 attggttacg gcctgaccca gcttgattta cacgctaccg acagcgatgc tctggtgctg
     2281 agctctaacg gtaaaagcga gaatgccgcc gatctcagcg caaagattac cggcagtggt
     2341 gacctggcat tcagcagcca gaagggtcag accgtatcgc tttctaacaa agacaacgac
     2401 tataccggtg ttaccgatct gcgcagtggg acgcttttgt tgaataacga taacgtgttg
     2461 ggtaataccc atgaactgcg tctggcggca gagactgaac tggacatgaa tggtcacagc
     2521 cagaccgtgg gcacgctcaa tggcagcgcc gattcactgc tgagcttaaa tggcggcagt
     2581 ctgacggtta ccaacggggg cacttcaacc ggttcgttaa cggggagcgg agagctgaat
     2641 attcagggcg gcacgctgga catcgcgggc gataacagca acctgacggc gaatgtgaac
     2701 attgctaatt cggctaatgt cctggtaagt catgcgcagg gattgggtag cgcaaacgtt
     2761 gagaacaacg gtaccctggc gttgaataat agcgctgaaa aaagagcggc tgcgtctgtg
     2821 aattacgccc tgggcggcaa tctgaccaac aacggtacgc tgatgaccgg aatgtcagga
     2881 cagcaagctg gcaatgtgtt agtggtgaag gggaactacc acggtaataa cggtcaacta
     2941 gtaatgaata cggtactgaa tggcgatgac tcagtaaccg ataaattggt tgtcgagggc
     3001 gatactagcg gcacgactgc cgttacggtg aataacgctg gcggtacagg tgcgaaaacc
     3061 cttaacggta tcgaacttat ccatgtagac ggtaagtctg agggcgaatt tgttcaggct
     3121 gggcgtatcg ttgcgggggc gtatgactac actctcgcgc gtggacaagg ggcaaatagt
     3181 ggtaactggt atctgaccag cggcagtgat tctcctgaac tgcagccgga gccagacccg
     3241 atgccgaatc cagagccaaa cccgaatcca gagccgaacc ctaacccgac acctacgccg
     3301 ggtccggatc tgaatgtgga taatgacctg cgaccggagg cgggtagcta cattgcgaac
     3361 cttgcagcag cgaataccat gttcaccacg cgtctgcatg agcgtctggg taatacgtac
     3421 tataccgaca tggtgacggg tgagcagaaa caaaccacta tgtggatgcg ccatgaaggt
     3481 ggtcataata aatggcgtga tggcagcggc cagctgaaaa cccaaagcaa tcgctatgtt
     3541 ctgcaactgg gaggcgatgt cgcgcagtgg agccaaaacg gcagcgaccg ctggcatgtt
     3601 ggggtcatgg cgggatatgg caacagcgac agcaaaacca tttcctcgcg aaccggttat
     3661 cgtgcaaaag cgagtgtgaa cggatatagc acaggcctct atgccacctg gtatgccgat
     3721 gacgagtcgc gtaatggcgc gtatctcgac agttgggcgc agtacagctg gtttgataac
     3781 acagtgaaag gggatgactt acaaagtgaa tcctataaat caaaaggatt taccgcttca
     3841 ctggaagctg gatacaaaca caaattagct gaatttaatg gcagccaggg aacgcgtaat
     3901 gaatggtatg ttcagccgca agcacaggtt acctggatgg gagtcaaagc cgataagcac
     3961 cgcgaaagca acggaaccct cgttcatagc aacggtgatg gcaatgttca aacccgactt
     4021 ggcgtaaaaa cctggctgaa gagccaccat aaaatggatg acggtaaatc ccgcgagttc
     4081 cagccgtttg tagaagtgaa ctggctacat aacagtaagg atttcagcac cagtatggat
     4141 ggcgtgtctg tcactcagga tggagcccga aatattgctg agataaaaac cggggtggaa
     4201 ggacagctaa atgccaacct gaatgtctgg gggaatgtgg gcgttcaggt tgccgatagg
     4261 ggatataatg acacctctgc aatggttggc attaagtggc aattctgatc tgaataatga
     4321 gtaaaaggag agagagccga acgggagata attcggctct ctgtgtcatt ttcttgcagc
     4381 cagctttatc cgtcagagtg aagactcgca gatttagctg gcgcacaaga tttaatcaag
     4441 gtgtgttcaa tggcttcgca tgatttgagt gtttttttgg aagagttcgg ggcaacggtg
     4501 aatttgacgt tgcctggtat cgtgtccgaa aaagaacggc ttttactcaa gctgctaatg
     4561 gagggaatgt cggttacaga aatctcacag tacagaaatc gcagtgcaaa gacaatttca
     4621 catcaaaaga agcagctgta tgaaaaacta ggcatccaga gtgatattac cttctggcgg
     4681 gatatctttt ttcaatatca tccgcaagtc attagtggca cgggaaataa aaataatttt
     4741 tatatccctg ataaccgttg tcaccatatc gtcacgcctg aagccatcag tctggcgttg
     4801 gaaaaccatg aatttaaacc gtggatccaa ccggttttct gcgcgcagac tggggtactg
     4861 acgggctgtg aagtgcttgt ccgctgggaa catccacaaa cgggaatcat cccgccggat
     4921 cagtttattc ctctggcgga gtcatccggt cttattgtca taatgacccg ccaactgatg
     4981 aaacagactg cggatattct gatgccggta aaacatttgc tgccggataa tttccatatt
     5041 ggtatcaacg tctcggcggg ttgttttttg gccgcgggat ttgaaaaaga atgcctgaac
     5101 ctggtcaaga aattaggaaa cgataaaatc aaactggttc tcgagctaac ggaacgtaac
     5161 cctattccgg taacgccaga agccagagcg atatttgaca gccttcatca gcataacatt
     5221 acctttgcgc tggatgactt tggtacgggt tatgcgacct atcgttatct gcaggcgttt
     5281 ccggtcgatt ttattaagat cgataagtca tttgtgcaaa tggcgggtgt cgacgaaata
     5341 tccggtcata ttgtggacaa tattgtcgaa ctggcgcgta agcctggtct gagtatcgtg
     5401 gcggaagggg tagaaaccca ggagcaggcg gatttaatga tcggtaaagg cgttcacttt
     5461 ttgcagggct atttgtactc tccgccagta ccgggtaata aatttatctc tgaatgggta
     5521 atgaaagcag gtggttgatg taaaccgcta ttcacagcgc atcgggaggt tggcagcgat
     5581 taattctccc gatgcagtaa atcctgatag atccccgtta atatgccatt tggcgcaaat
     5641 tgttttttaa tccactgcgt cacctgaccc gtagcagaat gctgagtcgc cagtaacatg
     5701 cgtgaatcct ggcgcgggtt atggatttgt cgggtaacca gcagggattg cgccatcgcc
     5761 tcacggacca tgtaatctgg caaaaaaaca atcccttcac ctaaaatctg gcactgacat
     5821 ttggtgttga aatctggcac cagaattgac tcctgcccgt gcagcaacca gcccactttt
     5881 ttattaatcg tatgcgcggt gtcctccacc ataatattag ggtagagacg cagttggctt
     5941 tcggcgatgg ttccggcaca aaggctaacg ggtgatccgg ggcgatagca aaagcccagc
     6001 gaatcgcgcc aatttcagtg taatcaatac cgccgccgtc cagcagcgtg tcgggtgcgc
     6061 cgatagcgat atttgcctga ttattaataa tcgcatccca gacgccgtta tacacttcgg
     6121 tggtgacggt aatctggcag gtaggaaact gctttttcag cacctgtaat aagcgtgcgg
     6181 tgtgtttggg cgtataaagc aactgattaa tacaaatgcg cacacgcgct tctatgccct
     6241 gcgaaatagt atcgatgctg cgtttgatgg cgtgaaaatc attcagcagg tcggtagctt
     6301 tgcggaaaaa ataacgcccg gactccgtta actcaatgct gcgggtactg cgagtgaaca
     6361 gcaccacatc cagccctgtt tccattcgct tgatggtgta gctaatggcg gaagtggtta
     6421 aacccaactc ttctgcggct ttactgaaac tgccaaaacg cgcagcggta gtaaatgcca
     6481 acagattctc ttcggtaaaa attgagttca ttaaaccatt cccgccatta tctaatatga
     6541 atttctccaa gcataactgt agaggtatca attagagatg ggtaatcatt tttgaataac
     6601 ttttaatacc cgtcgcgttt agtcgtcgct tcgcttaatt tttgtccgcc atattctgaa
     6661 attatcccca cgcagttacc cgccagaaaa cccagtaacg tcaccgtcca gttcatcccg
     6721 ctggcaaaaa acagcgtcat gccgagaaag ccaccgggaa taaatgacag taaccagaaa
     6781 cgcccttgcc agaccaccac cgccgagaag ggtacggtag taattacgct cgcccagaac
     6841 gcggacagtc cacttacaga ggccagccaa ccggcagcca gcgcggcgaa aaacgcccac
     6901 accatcccgg aataattaac cgacagactt ctgataaacc ccgatttacc gccgcctgcg
     6961 gcataaaaac tgcaaaaggc gacgaagcca atggttccta ataattccta ataattccca
     7021 tccctgagag agcccgacat gtgaagacac taactgccaa agccctgcac aaataccgac
     7081 ggtgacaccc gttgccgtga gtccgttcat cttgttcccc tctggttaga catggacgaa
     7141 agctactctt acatgtgcag actatagaga aataaaacct gattgagtga aagggtatcg
     7201 ggtcaaagaa acaaatattg cactaccgca cactgcgaaa agattgttga acatcattca
     7261 acaaaaaggc gaaatacggc gaattaaatt taagcgggtt cttttatcag attatcgttg
     7321 ctattctcaa ttcatcgaaa acagccacaa aaaccagggg aatgtgaatg agtataaaaa
     7381 atctacctgc cgactattta ttggctgcac aacagggtga tatcgataaa gtaaaaacct
     7441 gccttgcgct gggtgtcgat attaatacct gcgatcgtca ggggaaaacg gcaattacgc
     7501 tggcaagttt atatcagcaa tatgcttgcg ttcaggcatt aattgatgcc ggagcggata
     7561 ttaataagca agatcatacc tgtttaaatc cttttttaat tagctgtctg aacggtgatt
     7621 taacgctgct acgaattatt ttaccggcta aaccagacct taattgcgtc acccgttttg
     7681 gcggtgtcgg cctgacgcct gcctgtgaaa aaggccattt aagtattgta aaagagcttt
     7741 tggcgcatac ggaaattaac gttaaccaga ccaaccatgt tggctggacg ccactactgg
     7801 aagcgattgt gcttaacgat ggcgggatta aacagcaggc gattgtgcag ttattgctgg
     7861 aacacggtgc cagcccgcat ctgaccgata aatatggcaa aacgccactg gaactggcgc
     7921 gggaacgggg ctttgaagag attgcgcagt tactgattgc cgcaggtgca taaaccggga
     7981 ggcttgctat caacacacca gaaaaacggt gtgtgtgggc gctaactgcg gatgcggatt
     8041 ttctggcgca gcgagggcaa ggacaggttg aacaggtctt tgccagagcg gtaaatatcg
     8101 cactcccggc tcgccagcag ttgctgacgc tgctttgtga agagtacgac aatgcgccaa
     8161 acagttgtcg gttggcactc actcactttg atgatctgtt ccggcatggt gataaggttc
     8221 agtttgacga tcaaggtatt acggttggtc aacatcttca tatagagatg agtcgttgtc
     8281 ggcgttggct gtccccaacc ttgcaaatga ccgctgtgaa ttttcgcctt atcgcctggc
     8341 aacagtggca cgacatcatt catcagcacc tgggggaaaa tgaaaccctg tttaattatc
     8401 gcggcgataa tccgttttat caggcgttaa ataaagaatt acatattaaa cgacgggcag
     8461 ttatacaggc agtaatcgat aaacaaaata tcgcctcagc ggtcgccagt atgatgggct
     8521 tagggattgg ccttacgcca tcagccgatg attatttaac aggtctggca cttattttat
     8581 ttatttccgg gcatccggcg ggaaaataca aagaggaatt ttatctcggc ttgcaacgcg
     8641 gcagaaataa taccacgtta ttaagtgcca taacgctgga agccgcatta caacaacgct
     8701 gccgggaaaa tattcatcgt tttattcaca acattattta tgacatccct gggaacgcaa
     8761 ctcaggcaat agaaaaaatt aaacatattg gctccagttc cggctgcgac atgctgtatg
     8821 gcatggccga tggttgtgcg ctgagccaaa cctacggagg gaattatgtc agttaaaata
     8881 gtcattaaac cgaataccta ttttgattct gtctcgctga tgtctatctc cacgcgtgca
     8941 aataaactcg acggcgtcga gcaggcattt gtggcgatgg cgaccgaaat gaacaaaggt
     9001 gtgctgaaaa atttaggact gctgacgccg gagctggagc aggcgaaaaa cggcgacctg
     9061 atgattgtca tcaatggtaa atcgggggcg gacaacgagc agttgctggt ggagattgaa
     9121 gaactgttca acaccaaagc gcaaagcggc tcgcacgagg cgcgttacgc cactattgcc
     9181 agcgccaaaa agcatatccc ggaaagtaac ctggcggtga tttcggtcaa cggtctgttt
     9241 gccgctcgcg aagcgcgtca ggcgctgcaa aacgatctca acgtgatgct gttttccgat
     9301 aacgtctcag ttgaagatga actggcgctc aagcaactgg cccacgaaaa agggctgctg
     9361 atgatggggc cagactgcgg cacggcgatt atcaacggtg cggcgctctg ctttggtaac
     9421 gccgtgcgtc gcggcaacat cggtattgtt ggcgcatccg gcaccggcag tcaggagttg
     9481 agcgtccgca ttcatgaatt tggcggcggc gtttcgcaac tgattggcac cggcgggcgc
     9541 gacctgagcg agaaaatcgg cggcctgatg atgctcgacg ccatcgggat gctggaaaac
     9601 gatccgcaaa ctgaaatcat tgcgcttatc tccaaaccgc ctgcgcctgc ggtggcccgc
     9661 aaagtgctgg aacgtgcgcg cgcctgccgc aagccggtgg tcgtttgctt cctcggtcgt
     9721 ggcgaaacgc cagtggatga gcaggggcta cagtttgccc gcggcaccaa agaggcagcg
     9781 ctaaaagcgg tgatgctctc cggcgtgaaa caagaacatc tcgacctgca taccctgaac
     9841 cagccgttga ttactgacgt gcgcgctcgc ctgaagccgc agcagaaata cattcgtggc
     9901 ctgttctgcg gtggcacgct gtgcgatgaa acgatgttcg cggtgatgga aaaacacagc
     9961 gatgtctaca gcaacatcca gcccgatccg gaattccgcc tgaaggatat caaccgcagc
    10021 atcaaacaca ccttcctcga ctttggcgat gacgacttca ccaacggcaa gccgcacccg
    10081 atgattgacc ctaccaaccg catcagccgc ttgatcgaag aggcgcgcga tccagaagtg
    10141 gcggtgatcg tgatggattt tgtgctcgga tttggatcgc atgaagatcc ggtcggttcc
    10201 accatcgagg cgatcaaaga agcgaaagcg atcgccgctg ccgaaggacg cgagttgatc
    10261 attctcgcct atgtgctggg taccgatctt gatacgccat cgttagaaca acaaagccag
    10321 atgctgcttg atgccggcgt gattctggcg agcagcagca ccaataccgg attgctggcg
    10381 cgtgaattta tctgcaaagg ggaggaagcc tgatgagcca gtcactgttt agccaaccat
    10441 tgaacgttat taacgtcggc atcgccatgt ttagcgatga cctgaaaaag cagcatgtag
    10501 aagtgactca attcgactgg acgccgccgg ggcagggcaa tatgcaggtg gtgcaggcgc
    10561 tggataacat tgccgattcg ccgctggcag acaaaatcgc cgccgctaac cagcaggcgc
    10621 tggagcgcat tatccagtcg catccggtgc tgattggttt tgatcaggcg attaacgtgg
    10681 tgccgggcat gacgccgaaa accattcttc acgccgggcc gccgatcacc tgggaaaaaa
    10741 tgtgcggcgc gatgaaaggc gcggtcaccg gagcgctggt gttcgaagga ctggcgaaag
    10801 atctcgacga ggcggctgaa ctggcggctt ccggggagat caccttctcg ccgtgtcacg
    10861 agcacgactg cgtgggatcg atggcgggtg tgacctcggc ctcgatgttt atgcacatcg
    10921 taaaaaacaa aacctacggc aacatcgctt ataccaacat gagcgagcag atggcgaaga
    10981 ttttgcgtat gggcgctaac gaccagagcg tgattgaccg cctgaactgg atgcgtgatg
    11041 tgcagggacc aatactgcgc gacgcgatga aaattatcgg cgaaatcgat ctgcgcttaa
    11101 tgctggcgca ggcgctgcat atgggcgatg agtgccataa ccgcaataac gccgggacga
    11161 cgctgctgat tcaggcgctg acgccgggga ttatccaggc gggctattcc gtcgcgcaac
    11221 agcgcgaagt gtttgagttt gttgccagta gcgattactt ctccggcccg acgtggatgg
    11281 cgatgtgtaa agcggcgatg gatgcggcgc acggcatcga atacagcacc gtcgttacca
    11341 ccatggcgcg taacggcgtc gagttcggcc tgcgggtgag cggcctgccg gggcagtggt
    11401 ttaccggccc agcgcagcag gtcatcggcc cgatgtttgc tggttataag ccggaagatt
    11461 cggggctgga tatcggcgac agcgccatca cagaaaccta cggtattggc gggtttgcca
    11521 tggcgaccgc gcctgcaatc gtcgcgctgg tggggggcac ggtggaagag gctatcgatt
    11581 tctcccgtca gatgcgcgaa atcacccttg gcgaaaaccc caacgtcacc attccgctgc
    11641 tcggttttat gggcgtgccg tcggcagtcg acatcacccg cgtgggtagc agcggcattc
    11701 tgccggtgat caacaccgcc atcgcccata aagatgcggg cgtcggcatg attggcgcgg
    11761 gcattgtgca tccaccattc gcctgcttcg agaaagccat tcttggctgg tgcgaacgtt
    11821 acggcgtttg acctacctgt tcat
//
LOCUS       DBFIFS010000137        11766 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.137, whole genome shotgun sequence.
ACCESSION   DBFIFS010000137 DBFIFS010000000
VERSION     DBFIFS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.137"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..451
                     /gene="decR"
                     /locus_tag="N7A07_003247"
     CDS             <1..451
                     /gene="decR"
                     /locus_tag="N7A07_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU2477857.1"
                     /translation="KIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLEDDGI
                     LIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDY
                     LMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            481..2253
                     /gene="mdlA"
                     /locus_tag="N7A07_003248"
     CDS             481..2253
                     /gene="mdlA"
                     /locus_tag="N7A07_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU2477858.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            2246..4027
                     /gene="mdlB"
                     /locus_tag="N7A07_003249"
     CDS             2246..4027
                     /gene="mdlB"
                     /locus_tag="N7A07_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU2477859.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4208..4546
                     /gene="glnK"
                     /locus_tag="N7A07_003250"
     CDS             4208..4546
                     /gene="glnK"
                     /locus_tag="N7A07_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU2477860.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4576..5862
                     /gene="amtB"
                     /locus_tag="N7A07_003251"
     CDS             4576..5862
                     /gene="amtB"
                     /locus_tag="N7A07_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU2477861.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5911..6771)
                     /gene="tesB"
                     /locus_tag="N7A07_003252"
     CDS             complement(5911..6771)
                     /gene="tesB"
                     /locus_tag="N7A07_003252"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU2477862.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            6989..7561
                     /gene="ybaY"
                     /locus_tag="N7A07_003253"
     CDS             6989..7561
                     /gene="ybaY"
                     /locus_tag="N7A07_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU2477863.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7594..7905)
                     /locus_tag="N7A07_003254"
     CDS             complement(7594..7905)
                     /locus_tag="N7A07_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU2477864.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            8068..8164
                     /gene="ffs"
                     /locus_tag="N7A07_003255"
     ncRNA           8068..8164
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="N7A07_003255"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8284..8637
                     /gene="ybaA"
                     /locus_tag="N7A07_003256"
     CDS             8284..8637
                     /gene="ybaA"
                     /locus_tag="N7A07_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU2477865.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGTLRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8679..10229)
                     /gene="pdeB"
                     /locus_tag="N7A07_003257"
     CDS             complement(8679..10229)
                     /gene="pdeB"
                     /locus_tag="N7A07_003257"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU2477866.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(10393..10863)
                     /gene="ylaC"
                     /locus_tag="N7A07_003258"
     CDS             complement(10393..10863)
                     /gene="ylaC"
                     /locus_tag="N7A07_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU2477867.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(10979..11530)
                     /gene="maa"
                     /locus_tag="N7A07_003259"
     CDS             complement(10979..11530)
                     /gene="maa"
                     /locus_tag="N7A07_003259"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU2477868.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(11702..>11766)
                     /locus_tag="N7A07_003260"
     CDS             complement(11702..>11766)
                     /locus_tag="N7A07_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGU2477869.1"
                     /translation="ELTMNKLYDKIPSSVWKFIR"
BASE COUNT         2750 a         2863 c         3238 g         2915 t
ORIGIN      
        1 taaaattgac cgtaagctgc tggccttact acagcaggat tgcaccctct ctttgcaggc
       61 actggctgaa gccgttaatc tgacaaccac cccctgctgg aagcgcctga aacggctgga
      121 ggacgacggt atccttatcg gcaaagtcgc cctgctggat ccggaaaaaa taggcctcgg
      181 cctgaccgcc tttgtgctga taaaaacgca acatcacagc agcgaatggt attgccgctt
      241 tgtcacggtg gttaccgaaa tgccagaagt gctggggttc tggcgcatgg ctggtgaata
      301 cgattatctg atgcgcgtcc aggttgccga catgaaacgc tacgacgagt tttataagcg
      361 tctggtaaac agcgtgccgg ggctgtcgga cgtcacttcc agcttcgcga tggaacagat
      421 taaatacacc acttctttac ccatcgaata aatatccaga atcaggtcag gacacaacgc
      481 gtgcgattat ttgctcaatt aagctggtat ttccgtcggg aatggcgtcg ctatctcggg
      541 gctgtcgcct tgcttgtcat tatcgcgatg ctgcaactgg ttccaccaaa agtggttggt
      601 attgttgtcg atggcgtgac agaacaacac tttactaccg ggcagatcct gatgtggatc
      661 gccaccatgg tgctgattgc cgttgtggtt tatctcctgc gttacgtctg gcgcgtattg
      721 ctgtttggtg cgtcttatca actggctgtt gaactgcgtg aagattatta ccgtcagcta
      781 agccggcagc atcctgagtt ttacctgcgt catcgcacgg gtgacctcat ggctcgtgcg
      841 actaatgacg tcgatcgcgt cgtgtttgcc gccggagaag gggtgctgac gctggtggat
      901 tcactggtaa tgggctgcgc tgtgttgatt atgatgtcta cgcaaattag ctggcagttg
      961 accttatttg ccctgttacc gatgccagtg atggcgatca tgattaagcg caacggcgat
     1021 gctttgcatg aacgctttaa gctggcacag gcggcgtttt ccagtcttaa tgaccgcacc
     1081 caggaaagcc tcaccagtat ccgcatgatc aaagcctttg gtctggaaga tcgccagtcg
     1141 gcgttatttg ccgcggatgc cgaagatacc ggcaaaaaaa acatgcgggt ggcgcgtatt
     1201 gatgctcgtt tcgacccgac catctatatc gcgattggta tggcgaactt gctggcgatt
     1261 ggcggcggta gttggatggt ggtgcagggc agtttaacgc tgggccagct caccagtttt
     1321 atgatgtatt taggtctgat gatttggcca atgctggcgc tggcatggat gtttaacatt
     1381 gtggaacgtg gtagtgctgc gtacagccgt attcgcgcga tgctggcgga agcgccggtg
     1441 gtgaacgatg gtagcgaacc cgtgccggaa gggcgtggcg aactggatgt aaatattcac
     1501 cagttcacgt atccgcagac tgaccatcct gcgctggaaa acgtcaattt cgccctgaaa
     1561 cccggtcaga tgctgggtat ctgcgggccg actggttccg gcaaaagtac cctgttgtcg
     1621 ctcattcagc gtcatttcga cgtcagcgag ggggatattc gctttcatga tattcctctg
     1681 acgaagttac aactcgatag ctggcgtagc cgcctggcgg tagttagcca gacgccattc
     1741 cttttttctg acaccgtggc gaataacatc gcgctgggtt gcccgaatgc cacccagcaa
     1801 gagattgagc atgtcgcgcg gttagccagc gtacatgacg atattttgcg tctaccgcaa
     1861 ggttacgata cagaggtggg cgagcgcggt gtgatgcttt ccggcgggca aaaacagcgt
     1921 atctccattg ctcgtgcgtt attagtcaac gcggaaatcc tcatccttga tgatgcgctt
     1981 tcggcggtgg acggacgcac tgagcaccag atcctgcata acctgcgtca gtgggggcag
     2041 ggaagaacgg taatcatcag tgcccatcgc ctttctgcac tgacggaagc cagtgaaatt
     2101 attgtgatgc agcacggaca tatcgcccag cgtggcaatc atgatgtgct ggcacaacaa
     2161 agcggctggt atcgcgatat gtatcgctat caacaactgg aggcggcgct cgacgacgct
     2221 ccggaaaatc gcgaggaggc cgtcgatgcg tagttttagc caactgtggc cgactctcaa
     2281 gcgcctgtta gcgtacggtt caccgtggcg taaaccgctg gggattgcgg tcctgatgat
     2341 gtgggttgcg gcggcggcag aagtcagtgg gccgctgctt atcagctatt ttatcgacaa
     2401 tatggtagcg aaaaataacc tgccgttgaa agtggttgca gggctggctg cggcgtatgt
     2461 tgggctgcaa ctgtttgccg ccgggctaca ttacgcgcag tcgctgctgt ttaatcgggc
     2521 ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg gatgctgcgt tacgccagcc
     2581 attaagcgag tttgataccc aacccgtcgg gcaggtgatt tcccgcgtca ctaatgacac
     2641 tgaagtgatc cgcgatctct acgttaccgt agtggcaact gtcctgcgca gtgccgcact
     2701 ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg cgaatggcgc tggtggcgat
     2761 aatgattttc ccggtggtgc tggtggtaat ggtgatatac cagcgttaca gcacgccaat
     2821 tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac ggctttaacg aaatcatcaa
     2881 tggcatgagc gttatccagc agtttcgtca gcaggcgcga tttggcgaac gtatgggtga
     2941 ggccagtcgt tcacactata tggcgaggat gcaaaccctg cgcctcgacg gttttctgct
     3001 gcgtccgctg ctgagtctgt tttcatcgct cattctctgt ggcttgttga tgctgtttgg
     3061 cttctccgcc agcggcacca ttgaagtggg cgtgttgtat gcgtttatca gctatcttgg
     3121 gcgacttaac gaacccttaa tcgaactgac cacgcagcag gcgatgctgc aacaggctgt
     3181 tgttgctggt gagcgcgtgt ttgagctgat ggacggaccg cgccagcaat atggcaatga
     3241 tgatcgcccg ttacagagtg gcaccatcga agtcgataac gtgtcatttg cttatcgcga
     3301 tgacaatctg gtgctaaaga acattaatct ctctgtgcct tcgcgtaact ttgtggcgct
     3361 ggtcgggcat accggcagtg gtaaaagtac cctcgccagt ttattgatgg gctattaccc
     3421 gctaacggaa ggtgagattc gccttgatgg tcgtccatta agttcgctaa gtcacagcgc
     3481 gctgcgccag ggcgtggcaa tggtgcagca agatccggtg gtgctggcgg ataccttcct
     3541 cgccaacgtg acgctggggc gggatatctc cgaagaacgc gtctggcagg cgctggaaac
     3601 cgtgcaactg gcggagctgg cacgcagtat gagcgacggg atttatacgc cgctgggcga
     3661 gcaggggaat aatctctctg tcgggcaaaa gcaactgctg gcactggcgc gcgtgctggt
     3721 cgagacgccg caaatcctga tccttgatga ggcaaccgcc agcattgact ccggtactga
     3781 acaggcgatt caacatgctc tggcggcggt gcgtgaacat accacgctgg tagtgattgc
     3841 tcaccgctta tcgactattg ttgatgccga cactattctg gtgcttcatc gtgggcaagc
     3901 cgtggagcag ggcactcacc agcaactgct ggcggcccag ggacgctact ggcagatgta
     3961 tcaactgcaa cttgcgggcg aagagcttgc agccagcgtg cgtgaagaag aatcattgag
     4021 cgcctgaata gcgcaatatc tcatcgttgg tgcaaaaatg taacgcactg tgcactgtca
     4081 tagtgcgttt tcattttcaa tcttcttaac ttcctgctct ctttctcgtt tttcatttct
     4141 ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca ttaccgaatt ctgaccggag
     4201 gggatctatg aagctggtga ccgtgataat caaaccattc aagctggaag acgttcgtga
     4261 agcgttatct tccattggta ttcagggcct gaccgtcacc gaagtgaaag gtttcgggcg
     4321 tcagaaaggg catgccgagc tgtaccgggg ggcggaatac agcgtcaact tcctgccaaa
     4381 agtaaaaatt gatgtggcga ttgctgatga ccaactcgat gaagtgatcg atatcgtcag
     4441 taaggcggct tacaccggaa aaattggcga cggcaaaatc ttcgtcgctg aattgcaacg
     4501 cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg ctgtaatctc tggcacacag
     4561 caacaggaac gaaaaatgaa gatagcgacg ataaaaactg ggcttgcttc actggcgatg
     4621 ctgccgggac tggtaatggc tgcacctgcg gtggccgata aagccgacaa tgcgtttatg
     4681 atgatttgta ctgcactggt gctgtttatg actattccgg ggattgccct gttttacggt
     4741 gggttgattc gcggcaaaaa cgtgctgtcg atgctgacgc aggtgacggt gacgtttgca
     4801 ctggtctgta ttctctgggt ggtttacggt tactcgctgg cgtttggtga gggcaacaac
     4861 ttcttcggca acattaactg gttgatgctg aaaaacatcg aactgacggc ggtgatgggc
     4921 agcatttatc agtatatcca cgtagcgttt cagggatcgt ttgcctgcat taccgtaggc
     4981 ttgatagttg gggcgctggc ggaacgaatc cgcttctcag ctgtgttgat tttcgtggtg
     5041 gtatggctga cgctctctta cattcctatt gcgcatatgg tgtggggcgg tggtttgctg
     5101 gcttctcacg gtgcgctgga tttcgcgggt ggcacggtgg tgcatattaa cgccgcaatc
     5161 gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg gcttcggtaa agaggcgttt
     5221 aaaccgcaca acctgccgat ggtcttcacc gggactgcca ttctctatat cggttggttt
     5281 ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg cggcactggc atttgtgaat
     5341 actgtggtcg caacggcggc ggcaattctt ggctggatct tcggtgaatg ggcgctgcgt
     5401 ggtaagcctt cactgctggg agcgtgttct ggcgcgattg ccggtctggt cggcgtgacg
     5461 ccagcgtgtg gctacattgg tgttggcggc gcgttgatta tcggcgtggt agctggtctg
     5521 gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc gggtggatga tccctgcgat
     5581 gtcttcggtg tgcacggcgt ttgtggcatt gtcggctgta tcatgactgg gatttttgcc
     5641 gccagctcgc tgggcggcgt gggcttcgct gaaggtgtga cgatgggcca tcagttgctg
     5701 gtacagttgg aaagcattgc cattacgatt gtgtggtctg gtgtggtggc atttatcggc
     5761 tacaaactgg cggatctgac ggttggtctg cgtgtgccgg aagaacaaga gcgagaaggg
     5821 ctggacgtca acagtcacgg cgagaatgcc tataacgcat aacaagcact gcaaaaaaca
     5881 gccggacggt tttcacctcc ggctattttt ttaattgtga ttacgcatca ccccttcctg
     5941 aacggtcgag gcaaccagta cgccgtcctg ggtataaaac tcaccgcgca caaagccacg
     6001 tgcgctggac gccgaggtgc tctccacgct atacagcagc cattcattca aattaaacgg
     6061 gcgatggaac cacatggaat ggtcaatggt ggcaatctga atccccggtt cgagaaaacc
     6121 gataccgtgc ggctgtagag ctaccggcag gaagttaaga tcagaagcgt aaccgagcag
     6181 atactgatga acgcgcaggt catccggcac gctaccattt gcgcggatcc acacctgacg
     6241 atgtggttct gcgacgtgac ctttcagtgg gttatgaaac tccaccggac ggacttccag
     6301 cggacgatcg cagatgaatt tatctttaag cactggcggc agcaggtgcg ccagcgattg
     6361 ggcgatttgc gtttccgaag gcaggccatc aggcgctggc gcggacggca ttgttttttg
     6421 atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc atataaaaaa tcggtttgcc
     6481 gttttgaata gcagcaaccc ggcgggcgct gaagctgtta ccgtcacgca gcgtttcgac
     6541 atcataaata atcggcttct tactatcgcc agggcgaaga aagtagctgt gaaacgaatg
     6601 taccagccgc tcttcaggga cggtctcttt tgcagcatac aaggcctgac ccacgacctg
     6661 gccgccaaat acctggcgta aacctaaatc ttcactctgg ccgcgaaaga gtccttcctc
     6721 aattttttcc agatttaaca atgtcagtaa attttttagc gcctgactca tataactctc
     6781 cagtaacaaa gctgccgcag caagccaaag tgagttgagt ataacgcaaa tttgctactg
     6841 gtccgatggg tgcaatggtc tgaattacgg gctaattaca ggcagaaatg cgtgatgtgt
     6901 gccacaattg ttgacgttac tattttgtta accactcttc cggcgaggaa agttagcccg
     6961 ctggtgcatt gataataagg agaaatgaat gaaactcgtg cacatggcca gtggtttagc
     7021 ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg gatattcaga cgccagcccc
     7081 ggctgcaaat acgtctattt cagcaacaca acaacctgct atccagcaac cgaatgtctc
     7141 cggtaccgtc tggatccgtc agaaagtcgc actgccgcct gatgctgtgc tgaccgtgac
     7201 actttctgac gcgtcgttag ccgatgcacc gtcaaaagtg ctggcgcaga aagcggtgcg
     7261 tactgaaggt aaacagtcac cattcagctt tgttctgcca tttaacccgg cagatgttca
     7321 gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg aatgacaaac tggtatttat
     7381 taccgatacc gttcagccgg tgatcaacca ggggggaacc aaagccgacc tgacattggt
     7441 tccggttcag caaaccgccg tgcctgtcca ggccagtggt ggcgcaacaa ctaccgtacc
     7501 atcgacttca ccgacccagg tgaatccatc ttcggcagtt cctgccccta cgcaatacta
     7561 agcgttacta acccctttcc atctggagag gggttagtag ttccagcgat agcgctgtaa
     7621 gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct gtcgttgacg
     7681 ctgtaaatcc ggtccggtta gcgaaatggt gccgtggcga ttaaccaccc ggtgccaggg
     7741 caaggtgctg ccttcgggga gacgctttaa cacaccgcca acctggcgcg cggcgcgggg
     7801 cgatcccgcc agtttcgcca catcaccgta agtggtgaca tacccttcgg gaatagcggc
     7861 tacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt cttcctgaga
     7921 taatttttca gcataatctg gaaaaacgcc cgagtgaagc cgcattgcgc aagaaaccag
     7981 catctggcac gcaatgggtt gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt
     8041 ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac tctgtttacc
     8101 aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga tgtagctggc
     8161 agggccccca cccatttctg cctcccaccg tttcgtcaaa aaatcccaac atgactaaac
     8221 tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc ggaggtatgt
     8281 ctgatgaagt atgttgatgg ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat
     8341 cgggaaatgg ccgctaaggc cgcgccattg tttaaagagt ttggcacgct tcgtattgtc
     8401 gaatgctggg ccagcgatgt accggatggc aaagtgaccg attttcgtat ggcggtgaaa
     8461 gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa agaggtccgc
     8521 gacgcagcta atcaaaagat gatgtcggac ccacggatga aagagttcgg cgagtccatg
     8581 ccgtttgacg gcaagcgaat gatctatggc ggattcgagt caatcatcga cgaataggta
     8641 gcgtgacggg ctgtgcggcg tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag
     8701 cgtaagaaat cttctttcgg taatgccttg ctgtagagcc agccctgacc gtagtgcacg
     8761 ccatgctggc gtagccactc ttcttgttta ctggtttcga ttccctccgc taccattttc
     8821 agtttcagtg tttttgccat ttcgatgata tgcggcgtga catttttata ttccagcgca
     8881 tcaacgaaag atttatcgat cttcagaatg tcgacatcca gatcctgtaa ataacttaaa
     8941 cttgaatacc ccgtaccaaa atcatcaaga taaatttcat ggcccgcctc ccggtagcga
     9001 gaaattatcg gggcgctggt tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg
     9061 atctgtctgg gattaacctg atagtgattg atcatttcac gcagcaattg cgggattttt
     9121 tccgaggtga gcacggtgga ttcaagattg atcgaaatat gctgctgtgg atgctgacgc
     9181 agccagtcgc ccatatcttc aaagacgctt cttataatca gtagcgtcaa tggctcagaa
     9241 aggcccgttt gctgtgccag cggaataaaa ctatctggtg acaaccaact accgtctgtc
     9301 tgcggccagc gcgccagtgc ctcagcaccg acaattttgc cattggctaa ggagacaatc
     9361 ggctgatagt gcacgcaaat atcacgattt tcgatagcat cctgcagccg atgatgcggt
     9421 gactggatgc ggcgcaaaat gcgcagcaca aacatcgctg ccagcaggcc aatcaccaac
     9481 ccgagcggta accaaataaa gacttgccga tgccagcctt tctgcaacat tttcgatgaa
     9541 gcccaggtga taattgaaat attcatctcc gggaagggca ggatatcgta aatgattcca
     9601 ttattttcga tatgctcacc gggtgttttt tgtagcctgg taataattcc ctgagcaatt
     9661 tcatcgctgc tggttatgac aacgttatgg gcattgccaa taatggcggc atcaatttgc
     9721 catgagctat agggaatgac atcaataaag gaagcggggt cgatcatgac gacataatgt
     9781 gccgttccca tggcgaccat gtaacggata atgcctaaat cgttatgtga tgttaaccag
     9841 acacgataac catctttcga aattttacct ggctcgggga aggtatcggg cggactttca
     9901 tgctccagag acgaacactg gggaacgttg ttatcgatat aaaccacttc ctgaatatag
     9961 cgataactgt aagatacccg acgcatttcc atgagatggg cttcactaca gtcagcgcct
    10021 tgccatcttt ccagctcttg cagcgcatct ttcccttgtg tcgccacctt attggcccga
    10081 atagcgacgc gggaggaata ggtatccagc tcttcaataa acgatgtttc tacctgctga
    10141 tgggccagcc agatgcttaa gccgacaggc agcaatactg aaagaatcag tactcccgaa
    10201 ataaggccga ccagatgtcg tgttctcacc agcatatcct tattaacaca gtgtagggaa
    10261 agtatatctg attgtggaaa aaggctggca ggagataagg aggtagagct ggtatttcat
    10321 gaagtgatgg gtggaaacga tcgttgtcat ttgaccaggc gcagagcttt acgcctggta
    10381 tgtaatccta acttaagatg ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga
    10441 caattcacct ttacggacga tcattttttg cagttgttcc ttatgaacat ccgcgacacg
    10501 cggagagttc aagatggctt caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc
    10561 gccgttatag caaacgcgga aatccaacac gtcgatatct tcatagcggt agcgtgggta
    10621 gacatcgaaa aagaagaaac cattaagcag gataaataat acaaccagta gccagacaga
    10681 gcctgaaagc gtttcggact gcaacatcac cgccagggtg gcaaaaaaag caacgtacat
    10741 accgataaac agccccggat gtttacggat aaaactgata ctaaagcggg gtttgttgtc
    10801 gcgtttttcg cgggtattca gagactcaat cgtttcggtc agcaggcgtt gtatttcggt
    10861 catcgtttgc cttcgtggta tctgcaactt tccagggaac accctatttt aacgtgggaa
    10921 tagtagaggg aaagtaacag atctgccaca attttgcata acagttgcga aaaaccgatt
    10981 acaatttttt aattattctg gctggattac cgcccacgac aacgttgtcc gggacatctt
    11041 ttgtgacaac tgcacctgag gctaccacga cgttatcacc aatggtcaca ccagggttaa
    11101 tgaccgcgcg tccgccaatc cagacgttat taccgatggt gacgggtttc cccagttcag
    11161 caccgctatt acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata tgaacgccta
    11221 gtgccaacat acagttatca ccgatgcgaa tagggcagac atcaagcatc acgcaatcga
    11281 agttggcgaa aaaattatta ccgagaaaaa tgttatagcc atagtcacag cgaaacgttg
    11341 gctcaatata agcctctgtc acctgaccga atagatcagc gagaatttgc tggcgtaatg
    11401 tgtgctcttc cgccagggaa tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat
    11461 cgcgagataa cgtctcatct gccgagcgat acaactcacc agcaatcatc ttttcttttt
    11521 ctgtgctcat tactgacctc cggttgctga aaatgcggca acacagtaag acgaaaagtc
    11581 agtgaagaga acgttcccat tacatctgtg tgataaagat cacatagggt atgtcttcat
    11641 ggcgaaaaag tataaaattc ttaataaaca gccggttata gctccgaaag cgaattattt
    11701 attagcgaat aaatttccat actgaggaag ggatcttgtc gtacagttta ttcatggtca
    11761 attcgg
//
LOCUS       DBFIFS010000138        11763 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.138, whole genome shotgun sequence.
ACCESSION   DBFIFS010000138 DBFIFS010000000
VERSION     DBFIFS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.138"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..498
                     /gene="ftnA"
                     /locus_tag="N7A07_003261"
     CDS             1..498
                     /gene="ftnA"
                     /locus_tag="N7A07_003261"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU2477870.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(535..774)
                     /locus_tag="N7A07_003262"
     CDS             complement(535..774)
                     /locus_tag="N7A07_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU2477871.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            966..2177
                     /gene="tyrP"
                     /locus_tag="N7A07_003263"
     CDS             966..2177
                     /gene="tyrP"
                     /locus_tag="N7A07_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU2477872.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2239..2904)
                     /gene="yecA"
                     /locus_tag="N7A07_003264"
     CDS             complement(2239..2904)
                     /gene="yecA"
                     /locus_tag="N7A07_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU2477873.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3100..3186)
                     /locus_tag="N7A07_003265"
     tRNA            complement(3100..3186)
                     /locus_tag="N7A07_003265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3150..3152),aa:Leu,seq:taa)
     gene            complement(3199..3272)
                     /locus_tag="N7A07_003266"
     tRNA            complement(3199..3272)
                     /locus_tag="N7A07_003266"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3238..3240),aa:Cys,seq:gca)
     gene            complement(3326..3401)
                     /locus_tag="N7A07_003267"
     tRNA            complement(3326..3401)
                     /locus_tag="N7A07_003267"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3366..3368),aa:Gly,seq:gcc)
     gene            complement(3553..4101)
                     /gene="pgsA"
                     /locus_tag="N7A07_003268"
     CDS             complement(3553..4101)
                     /gene="pgsA"
                     /locus_tag="N7A07_003268"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU2477874.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4158..5990)
                     /gene="uvrC"
                     /locus_tag="N7A07_003269"
     CDS             complement(4158..5990)
                     /gene="uvrC"
                     /locus_tag="N7A07_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU2477875.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5987..6643)
                     /gene="uvrY"
                     /locus_tag="N7A07_003270"
     CDS             complement(5987..6643)
                     /gene="uvrY"
                     /locus_tag="N7A07_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU2477876.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6785..6901
                     /locus_tag="N7A07_003271"
     CDS             6785..6901
                     /locus_tag="N7A07_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2477877.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            6939..7115
                     /gene="yecU"
                     /locus_tag="N7A07_003272"
     CDS             6939..7115
                     /gene="yecU"
                     /locus_tag="N7A07_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU2477878.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7102..7326
                     /gene="yecF"
                     /locus_tag="N7A07_003273"
     CDS             7102..7326
                     /gene="yecF"
                     /locus_tag="N7A07_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU2477879.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDEPQAAVFSKTVKQIKQAYRQ"
     gene            complement(7394..8116)
                     /gene="sdiA"
                     /locus_tag="N7A07_003274"
     CDS             complement(7394..8116)
                     /gene="sdiA"
                     /locus_tag="N7A07_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU2477880.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8346..9098)
                     /gene="tcyN"
                     /locus_tag="N7A07_003275"
     CDS             complement(8346..9098)
                     /gene="tcyN"
                     /locus_tag="N7A07_003275"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU2477881.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(9095..9763)
                     /gene="tcyL"
                     /locus_tag="N7A07_003276"
     CDS             complement(9095..9763)
                     /gene="tcyL"
                     /locus_tag="N7A07_003276"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU2477882.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9778..10764)
                     /gene="dcyD"
                     /locus_tag="N7A07_003277"
     CDS             complement(9778..10764)
                     /gene="dcyD"
                     /locus_tag="N7A07_003277"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU2477883.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10869..11669)
                     /gene="tcyJ"
                     /locus_tag="N7A07_003278"
     CDS             complement(10869..11669)
                     /gene="tcyJ"
                     /locus_tag="N7A07_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2477884.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT         2893 a         3085 c         2751 g         3034 t
ORIGIN      
        1 atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct
       61 tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc
      121 gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac
      181 ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat
      241 tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa
      301 attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg
      361 cagtggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa
      421 ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc
      481 ctcgacacac aaaactaatg ctctccggcg gcaggtttct gccgccattt caaatcagtg
      541 acggcaaatc ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt
      601 tgctgccaga aacgctacca gctctcccgc tgtcatccct tctgccgagc aagtgtgaaa
      661 acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg
      721 ctcgcctgat tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc
      781 tcctcaaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcagg
      841 actgaatgct ttttattgta catttatgtt tacaccatat gtaacgtcgg tttgacgaag
      901 cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag
      961 aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg
     1021 caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct
     1081 tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc
     1141 agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg
     1201 gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca
     1261 tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt
     1321 cggcaaccgc tggcgtgttg ttgttcactt ttgttgccgg tggcgtggtt tgtgtcggga
     1381 catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg
     1441 tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc
     1501 aacaggggct ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta
     1561 gcgtgccgag tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta
     1621 taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca
     1681 gcattgattc aacaaccttt atgggattgc tggctaatca tgccggatta aacgggctgt
     1741 tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg
     1801 ctgatttagc ccttgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg
     1861 cagatttgtt tcagcgttca aataccgttg gtggacgatt gcaaactgga gcaattacct
     1921 ttctgccacc gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt
     1981 acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa
     2041 gcagaaagca caatcctcaa gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg
     2101 tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt
     2161 taccagaagt ggggtgatta gatagcctca aattccttat tgggtgccag aattaacgct
     2221 gacacccaat ttgacttctt aatgcaggca gcactgctta aattttttac cactaccgca
     2281 cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt
     2341 ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc
     2401 tacgctctct tcaaacgctt ctggcgacat cttctctacc cgctcgaagt tttcctcagt
     2461 accgtgcagc gctatcgcct ccagcgctgg ttttaacgaa tcgggcaatg ttgaccagtc
     2521 agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct
     2581 gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg
     2641 ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc
     2701 tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg
     2761 ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc
     2821 aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact
     2881 ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct
     2941 atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa
     3001 tcaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg
     3061 acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg
     3121 aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat
     3181 ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc
     3241 aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat
     3301 ccgctgacgc cgatgtcttt taaactggag cgggaaacga gactcgaact cgcgaccccg
     3361 accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt
     3421 aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta
     3481 cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt
     3541 gcgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat
     3601 tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt actcaaccca
     3661 aatgttcgga cgccacagca gccatgccaa cgccaccatc tgggcagtgg ttttcacttt
     3721 cccaatccag gagacagcca cgctactgcg tttacccaac tccgccatcc attcgcgtag
     3781 cgcagaaata ataatttcac gggcgatcat cgttgccgcc ggtaaggtca cccaccagct
     3841 gtggtaatgc tcggttacca gcaccatggc gatagccacg agaactttat ctgctacagg
     3901 gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa
     3961 ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaagtgac
     4021 aggcagataa aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa
     4081 cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct
     4141 atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc
     4201 gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg
     4261 cccatatatt tcaacaacat ttgccggcgt tttggcccga cgccttcaat ggtttccagg
     4321 gaactggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt
     4381 gattcatcgc gaatatgctg gataacatgc agcgcgggag aatctggcgg caaactgaat
     4441 ccctcacctt ccggctcaaa gaacagcgtt tccagcccag ccttacgatc tgctcctttg
     4501 gcaacaccaa gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca
     4561 tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa gtatcacatc cgggatctta
     4621 ctgtcgtcga tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca
     4681 tcgcccggcg tgatgccagt aatgttatag cgccgatact ccgcacgcag cgggccgtta
     4741 gcatcaaaca ccacacagga agcgacggtt tgttcaccca tggtatggct gatgtcaaag
     4801 cactccatcc gcttcacttc cggcaatttc aacacgctgg caagtgctgt aagccgctgg
     4861 tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg
     4921 agtttcaggt aacgcgccct atcgccacga ggtttggttt gaacattaat cttgcgtccc
     4981 gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg
     5041 atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactggcc tacgaaggtt
     5101 tctaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg
     5161 agcactttgc cctgacgaat gaacaatacg tggacacaag ccatacccgc atcgaacgcc
     5221 acaccaataa cgtcgaggtc gtcgccggta ttggaaacga attgtttttc ggtgacgcgt
     5281 cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt caaactccag attctgactg
     5341 gcagtttcca tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac
     5401 aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac
     5461 ggtcccagac agcgccctat ctgatattgc agacacggac gcgagcgatt gcgataaaca
     5521 ctattttcgc actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg
     5581 gcatagccat tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc
     5641 atcgccagac gcgggtgggt atcgccactc aggaagataa aaggatatga tttatcatcg
     5701 cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc
     5761 gcttcggttt ctgtatgagt aaccgttaca tcaatttgct ggatctgggc gaccagcgct
     5821 tcggttttgc gcgaagcgag gttgctacgg aaatagctgg aaagccgttt tttcaggtct
     5881 ttcgctttgc cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgccaggc
     5941 tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata
     6001 atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat
     6061 gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat
     6121 tgagctgttc tgagatctca ttgaccttct ggcccttcgt gatcatcagc ataatctgca
     6181 attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta
     6241 atgccatttg ttgagcgatg tcagaagcaa tgtaacgctg cccagaatag acagaacgga
     6301 tcgcactcac gacttcctgc ggagccgcgc ctttgctgag gtagcccgca gcaccggcct
     6361 gcatgacttt cgctggtaaa gggttttctg tatggacggt aagcatgatg atttttacat
     6421 cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca
     6481 tgtccattag caccacgtca acggcatttg cccggcacca cttaacggcg tcttcaccgc
     6541 acgatgcctc accgacgact tttataccct ttatatcttc cagaatgcgt cgtatccctg
     6601 cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa
     6661 tagggataac gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg
     6721 aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac
     6781 agttatggtc acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctgcgac
     6841 gaaatgtgac aaaaattacc tttattcagc aaaaatgaaa atcagcccgc tgaatatgta
     6901 acattaatta acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc
     6961 attatattaa cgcatttaat agcaccgttg acggcacgct caaaaaacaa ccactgctat
     7021 ttttagcaaa gcttatggta tactccgccg ccttaacatt tttcaccgca aattttcatt
     7081 gcaacatgac gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa
     7141 gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg
     7201 ggacaagctg acgcgggccg cgtgatgtta aagatggaac aacagcttgc gctgatcgaa
     7261 gacgaacccc aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt
     7321 cagtaatata aaaccggctg atagcgtgcc tttcagccgg tttttgcatc tggcacgcag
     7381 gacagaaaag agatcaaatt aagccagtag cggccgcgta acaggcaacc tgggtcttat
     7441 ttggtgcatt aatttttttc tgcatgtttt tctggtggaa attgaccgta ttctcagaga
     7501 ttgacaaaat catcgctatc tctgctgatg tcttcccttc cgccgtccac ctcagaattt
     7561 ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca
     7621 tcagagccat cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa
     7681 gaatgggtat ttcgcgcgcg ctgcaacggg aaaaggacaa aaagcccagc gcccggtttg
     7741 gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata
     7801 acggctgtgc ttcgttgaat aagtcatcat tccacattag atggccctga ctaaagtttt
     7861 cagggttcag caccggatca attgcgagaa agttttttgc ctgataataa ctaacccacg
     7921 cctcagggta attggtgtag aaagccactt taggtcgagt gaatggtacc gggtggcgga
     7981 cacataacga atagtaatcg tactccagct gctgagcctg aagctcaatt tcatggtaga
     8041 cctcttctgc ggtctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa
     8101 aatccgtatc ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat
     8161 aattcaaact atcacataaa taagatttat atataattta tattattcag gcaatgaatt
     8221 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat tttttaagca
     8281 gttttatcag gcttgaaatg gcgttccgcc ccgactgtcg aatcgtcggg gctgattttt
     8341 tcttattatt gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctcgggatcg
     8401 gcaaataacg cttttgcggc cccctgctcg actatccgcc cctggtccat aaaaatcgcc
     8461 cggtccgcaa catcccgggc aaagctcatt tcgtgtgtca caatcaccat cgtgcgcttt
     8521 tcctgcgcca gctgacggat ggtgttcagg acttcgccca ccagctctgg atccagcgct
     8581 gacgttggct cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg cgcaatcgca
     8641 acacgctgct gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc
     8701 ccaactttcg ccaacagctt gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
     8761 acgatcaccg gcccttcaat aatgttctcc agcaccgtac gatgcggaaa caaattaaag
     8821 ttctggaaga caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt
     8881 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt
     8941 tccagcagat ttatgctacg tagcaatgtg gttttgccgg aaccactcgg accgataatt
     9001 gccaccactt cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtgg
     9061 aattttttca ctaggttctt aacttcaatg gcactcattt gggttctctc tcctggcgat
     9121 taagttgatt ctcaaaatgg ttctgcaacg tcgataacac agtcgccatg atccagtaga
     9181 tgagcgaagc cgccagatac atggtgaata cctccagcgt acgcgaggta atcaactgcg
     9241 cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatct tttaccagac
     9301 tgataaagct gttcgagagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc
     9361 gacgcatagt ctgccatggc gtcataccaa tactggcagc tgcttcccac tgacctttat
     9421 caatagaaga aatggcggcg cgaagcgttt cggcagtata ggcggcagtg ttgagcgaca
     9481 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat
     9541 agatcataaa cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg
     9601 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aaccctagca
     9661 gtaaaccaaa aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca
     9721 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
     9781 aacgtgggga tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg
     9841 cccttcatct ttgaagcgtt tctgactgat accgtcaatc agacccgcca tcgcttttcc
     9901 ggtatacaca ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat
     9961 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc
    10021 cactgatgcg gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg
    10081 caattgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag
    10141 gtgttccagc ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac
    10201 cgatgaaata ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata
    10261 acctagcgcg ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
    10321 tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca
    10381 catttcaatc tgggtattga acagatccag caacaagcga ttgccgttgg ttaaatagtt
    10441 ttctgcggtt gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttggc
    10501 agcgactgcg gcagtctgcc gcacatggtt agactggatc gccccggcag taatcagcgt
    10561 atcggcacct tcacgcagag catctgccgc gagaaattct agcttacgta atttattgcc
    10621 gcccattgcc atgggggtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc
    10681 agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca gccgtggaaa
    10741 acgggttaaa ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttctcat
    10801 tatgcacgct gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat
    10861 tatgctgatt atttggtcac atcagcacca aaccattttt cggaaagggc ttgcaaagtg
    10921 ccgtcttttt gcatttccgc aattgcatca ttcactgctt tcagcaggtc ttcatttcct
    10981 ttacgcagcg ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta
    11041 tcgttggttt tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata
    11101 cgccctacgc gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg
    11161 ccctgaacat tctgccgcag ccactcttca tagttggtgc ccagaccgac ccccactttt
    11221 ttgcctttca gatcatcggc tgttttaatg gtgccttcgt tacctttttt caccagcgcc
    11281 tgaataccag aaatggtgta cggggttgag aaatcgtatt ttttcttgcg ctcatcagaa
    11341 atggtgacct gattaatcac cacatcaata cgtttagagt ccagcgacgc cagcataccg
    11401 tcccatttgg tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca
    11461 aattccactt caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa
    11521 gtaccttcca gccctaccag cagcgtgccg cgctctttaa ctttattaag cagaccttca
    11581 tctgcaaaac ttttaacgct catgcccgca atcagcgcta cggccatcac acccatcaat
    11641 gcctgacgtc ccagatgtgc taatttcata ttcaccccga atgttgttat gtctgtttgc
    11701 agtgtagagc cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa
    11761 tat
//
LOCUS       DBFIFS010000139        11639 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.139, whole genome shotgun sequence.
ACCESSION   DBFIFS010000139 DBFIFS010000000
VERSION     DBFIFS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.139"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            51..653
                     /gene="yebB"
                     /locus_tag="N7A07_003279"
     CDS             51..653
                     /gene="yebB"
                     /locus_tag="N7A07_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU2477885.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(655..1176)
                     /gene="ruvC"
                     /locus_tag="N7A07_003280"
     CDS             complement(655..1176)
                     /gene="ruvC"
                     /locus_tag="N7A07_003280"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU2477886.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1211..1951)
                     /gene="yebC"
                     /locus_tag="N7A07_003281"
     CDS             complement(1211..1951)
                     /gene="yebC"
                     /locus_tag="N7A07_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2477887.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1980..2432)
                     /gene="nudB"
                     /locus_tag="N7A07_003282"
     CDS             complement(1980..2432)
                     /gene="nudB"
                     /locus_tag="N7A07_003282"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU2477888.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2550..4322)
                     /gene="aspS"
                     /locus_tag="N7A07_003283"
     CDS             complement(2550..4322)
                     /gene="aspS"
                     /locus_tag="N7A07_003283"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU2477889.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4632..5198
                     /gene="yecD"
                     /locus_tag="N7A07_003284"
     CDS             4632..5198
                     /gene="yecD"
                     /locus_tag="N7A07_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU2477890.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5195..6013
                     /gene="yecE"
                     /locus_tag="N7A07_003285"
     CDS             5195..6013
                     /gene="yecE"
                     /locus_tag="N7A07_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU2477891.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6066..6461
                     /gene="yecN"
                     /locus_tag="N7A07_003286"
     CDS             6066..6461
                     /gene="yecN"
                     /locus_tag="N7A07_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU2477892.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6502..7245
                     /gene="cmoA"
                     /locus_tag="N7A07_003287"
     CDS             6502..7245
                     /gene="cmoA"
                     /locus_tag="N7A07_003287"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU2477893.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7242..8213
                     /gene="cmoB"
                     /locus_tag="N7A07_003288"
     CDS             7242..8213
                     /gene="cmoB"
                     /locus_tag="N7A07_003288"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU2477894.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8378..10807)
                     /gene="torZ"
                     /locus_tag="N7A07_003289"
     CDS             complement(8378..10807)
                     /gene="torZ"
                     /locus_tag="N7A07_003289"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU2477895.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10832..>11639)
                     /locus_tag="N7A07_003290"
     CDS             complement(10832..>11639)
                     /locus_tag="N7A07_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU2477896.1"
                     /translation="KIDSDDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIAS
                     QSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIY
                     PFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATL
                     SEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGC
                     HAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2619 a         3093 c         2874 g         3053 t
ORIGIN      
        1 attttttatg ctattacgta tattcgtatc tatttcaatg gaatgacaac gtgaatatta
       61 attaccctgc tgaatatgaa attggagata aagtctttac atgtataggt gctgccttat
      121 ttggtcaaat atcagctgca tcaaattgct ggagtaatca cgtcgggatc attatcggtc
      181 ataacggtga agacttcctg gttgcagaaa gccgggttcc cctttcaacc atcaccacgc
      241 tatctcgttt tattaaacgt tcgtctaatc aacgctatgc tataaagcga ttagacgctg
      301 ggctgacaga acaacaaaaa caacgaattg ttgaacaggt tccctcccgg ctacgcaaac
      361 tttaccacac cggttttaaa tacgaatctt cgcgccagtt ctgttcaaaa tttgtttttg
      421 atatttataa agaagcgcta tgtattccgg tgggtgaaat agagacgttt ggagaattgt
      481 taaatagcaa tccgaatgca aaactcactt tctggaaatt ctggttcctg ggttctattc
      541 cgtgggagcg taaaaccgtc acgccagcca gtttgtggca tcatccaggt ttggtgttga
      601 ttcatgcgga gggagtggaa acgcctcagc cggaactgac cgaggcggta taacttaacg
      661 cagtcgccct ctcgccaggt tcagccgcga ttcgctcatc tgcatcgcat tctgactaac
      721 gtggcagtgg gtgatggcaa tcgccagcgc atcggcggca tccgcctgtg gattagcggg
      781 cagtttcagc aaggtgcgga ccatatgctg cacctggctt ttttcggcac taccaatacc
      841 taccaccgtt tgctttacct gacgtgccgc gtattcaaat actggcaact cctgattcac
      901 cgccgccaca atcgccacgc cgcgcgcctg gcccagtttc agggctgagt cagcgttctt
      961 tgccataaag acttgttcaa tggcgaaata atcgggctgg aactgggtga tgatttccgt
     1021 cacgcccgca tagatgagtt tcagacgaga cggtaaatca tccactttgg tgcgtatgca
     1081 tccgctaccc aggtaggaca gttgcctacc tacctggcgg atgacgccgt agccggtcac
     1141 gcgcgaaccc ggatcaatgc cgagaataat agccatcacg cgtctccgtt ttgctgttta
     1201 gcaggcctca tcagagagtc gctgcgacct catcagagat ttcaccgtta tggtaaactt
     1261 cctgcacgtc gtcgcaatct tccagcatat cgatcagacg catcagtttc ggtgcggttt
     1321 ctgcatccat atcagctttg gtagacggga tcatggaaac ttccgcgctg tctgctttca
     1381 gacctgccgc ttccagagcg tcgcgcactt tacccatttc ttcccatgca gtgtagacat
     1441 caatcgcgcc gtcatcatag gtcacaacgt cttcagcacc tgcttccagt gctgcttcca
     1501 tgatggtgtc ttcatcgcct ttctcgaagg agatgacgcc ttttttgctg aacagatagg
     1561 ctacggaacc atcagtaccg aggttaccgc cacatttgct gaatgcatga cgcacttcag
     1621 caacggtacg gttgcggttg tcagacagac attcaatcat gattgccgtg ccgccaggac
     1681 cgtaaccttc gtagatgatg gtttccatgt tcgcatcatc atcaccgccc acaccacgtg
     1741 caattgcgcg gttcagagtg tcacgggtca tgttgttaga cagtgcttta tcaattgctg
     1801 cacgcagacg cgggttagcg tccggatcgc caccgcccag cttagccgcg gttaccagct
     1861 cacgaatgat tttagtgaag attttaccgc gcttagcatc ctgcgcagct ttacgatgtc
     1921 tggtgttggc ccatttacta tgacctgcca taaaaatatc tccagatagc cctgcctgtt
     1981 caggcagcgt taattacaaa ctgttcaatc gcctgccggt tgctccagga cttagtgagc
     2041 gccgccgcag caggcgcatc aagccacttg taagccagat gttcagtgaa aacgatctgc
     2101 cgctcatgcg gaagcgcaag acagaaccat gattccgtat tacgcgtcac gcccggcgca
     2161 tagcgatgac gtaaatgtga aaaaatttca aactctaccg tgcgctgaca gtcaattaag
     2221 gtcagttgtt cagcgacaac atcaatggtg acctcttcct ttacttcgcg catggcagct
     2281 tgcggcgcgg tttcaccctc ttccacgctg ccggttaccg actgccagaa atcggggtca
     2341 tcacgccgct gcaacatcag cacccgtttc gtatcttgtg cgtagatgac cactaagatc
     2401 gaaacgggac gcttatacac tttatccttc acgctgcctc ttcgttgact gccttcgctc
     2461 atcccattca catagttatc tatgctcatg ggagttcact cagttgccgc ctcgatgcaa
     2521 cgcgaatgat ttcgtgtatt tgagtcatat cagttattct cagccttctt cacaacctga
     2581 atgctcagct cagccagtgc agccgggtta gcaaagctcg gtgcttcagt catcagacac
     2641 gctgccgccg tggttttcgg gaaggcgata acgtcacgga tattgtcggt gccggtcagc
     2701 agcatggtca gacggtcaag accgaatgcc agacctgcgt gcggcggagt accgtatttc
     2761 agagcgtcga gcaggaagcc gaatttctcg cgctgttcct cttcgttgat acccagaata
     2821 ccaaacaccg tctgctgcat atcaccatta tggatacgta ctgaaccacc gcccacttcg
     2881 taaccattga tgaccatatc gtaagcgttc gccaccgcat tttccggtgc agctttcagc
     2941 tctgcagccg tcatgtcttt cggtgaggtg aacggatggt gcattgccgt caggccgcct
     3001 tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca gcggtgccca tttgctttcg
     3061 tcggtcagac caaggtcttt acccactttc aggcgcagtg cgcccatcgc gtcggcaaca
     3121 attttcttgt tgtcggcacc gaagaaaatc atatcgccat cttgcgcggc agtacgatcc
     3181 aggatggctt cgatgatttc tgcattaagg aacttcgcta ccgggctgtt gataccttcc
     3241 agacctttcg cgcgttcgtt aactttgatg taagccagac ctttcgcgcc gtagatttta
     3301 acgaagttac cgtattcgtc gatctgctta cgggtcagcg atgcgccacc cggaacacgc
     3361 agagcggcaa cgcgaccttt cggatcgttc gccggacctg caaatactgc aaactcaaca
     3421 gatctcagca gatcagcaac gtcggtcagt tccatcgggt tacgcagatc cggtttatca
     3481 gaaccataac ggcgttcggc ttccgcaaag gtcattactg ggaaatcgcc cagatccacg
     3541 cccttcactt ccagccacag atggcgcacc agcgcttcca tcacttcacg cacttgcggc
     3601 gcggtcatga aagaagtttc cacatcgatc tgagtaaatt caggctgacg atcagcgcgc
     3661 aggtcttcgt cacggaagca tttaacgatc tgatagtagc ggtcaaagcc ggacatcatc
     3721 agcagctgtt tgaacaactg tggggattgc ggcagtgcgt agaatttacc tttgtgcaca
     3781 cgagaaggca ccaggtagtc acgcgcgcct tccggcgtgg ctttggtcag catcggagtt
     3841 tcgatgtcga ggaaaccgtg gtcatccata aaacggcgca ccaggctggt gattttagcg
     3901 cgggttttca ggcgctgagc catttccgga cgacgcaggt cgaggtagcg gtatttcaga
     3961 cgcgcttctt cggtgttgac gtggttagag tcaagcggca gaacatctgc gcggttgatg
     4021 atagtcagcg aggacgccag cacttcgatt tcgccggtcg ccatatcgcg gttaatattt
     4081 ttttcgtcac gcgcacgtac ggtgcccgtg acctgaatgc agaactcatt acgcagttca
     4141 gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac gataccttcg
     4201 cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac gacgacggtt gacccaacca
     4261 cacagagtca cctgctgccc cacgtgggac aaacggagct gtccacaata ttctgtacgc
     4321 atgagatatc ccttaactta gctgccggcg gatgccccct gctgcgcagg tgaccaagtc
     4381 gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg gctattatac tggaaattct
     4441 gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt taccacttta tatcgggcct
     4501 gaaatcagac tctacgccag tttgctataa aggtgttgcc cgaactcata caaattaaca
     4561 aaatttgtcg ttccgccatc ggctaatcgc attaaggtgt gaggcacgat tttgtttttt
     4621 caggagtcat catgcttgaa cttaatgcta aaaccactgc gctggtggtg atcgatttac
     4681 aagaaggcat cttacctttt gccggtggtc cacatactgc cgatgaggta gttaatcgcg
     4741 ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc cgtgtttctg gtgcgcgttg
     4801 gctggtctgc cgattacgcc gaagcattaa aacagccggt tgatgcccct tcccccgcta
     4861 aagtgttgcc cgaaaactgg tggcaacatc cagctgcatt aggtgcaact gacagtgata
     4921 tcgaaataat caaacgtcag tggggtgcgt tttacggtac ggatctggag ttgcaattac
     4981 gccgccgagg tatcgataca atagtgttat gtgggatctc gaccaatatc ggagttgaat
     5041 ccaccgcccg caatgcctgg gaacttggct ttaatctggt gattgccgaa gatgcctgta
     5101 gtgccgctag cgccgagcag cacaataaca gtatcaacca tatctacccg cgcatcgccc
     5161 gtgtgcgtag cgtggaagag atcctcaacg cgttatgatt tacatcggtc taccgcaatg
     5221 gtcgcatcct aaatgggtgc ggttggggat caccagcctt gaagagtatg cccgtcactt
     5281 taactgcgtg gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg
     5341 ctggcgtgaa cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc
     5401 gcatcaggca gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc
     5461 accgttggct ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg
     5521 ggagctgcct gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt
     5581 ggaagtccgc catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg
     5641 tttacatcag cgcggcgtta atcaggtgat tttagacagc cgcccggttc atgcagcacg
     5701 tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc
     5761 tgtactgacg gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa
     5821 ccgggaatta tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc
     5881 ttatcttttt ttacatacgc cagatattgc ccaggccccg gaactggtac ataccctgtg
     5941 ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc
     6001 ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag
     6061 tttgtatggt aagcgcgctg tatgccgttt taagtgcgtt gttattaatg aagttctctt
     6121 ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg
     6181 aactgcaaag cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg
     6241 tgctgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca
     6301 tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgttccgct
     6361 ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc
     6421 tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc
     6481 tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc
     6541 agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag
     6601 cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc
     6661 gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg
     6721 gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg
     6781 atgattgaac gctgccgtcg tcatattgac gcctataaag cccctacgcc agtagacgtc
     6841 attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt
     6901 accctgcaat tcctggaacc ttccgagcgc caggcgttac tggataaaat ttatcaaggg
     6961 ctgaaccccg gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt
     7021 ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg
     7081 gagatcagcc agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc
     7141 cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt
     7201 aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact
     7261 tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga
     7321 ttgctaactg gcagcgcgag cagcagcacg ggttgtttaa gcagtggtca aatgcggtgg
     7381 agtttctacc tgaaattaaa ccgtatcgtc ttgatttatt gcatagcgtt acggcagaaa
     7441 gcgaagagcc actgagcgca gggcaaatta aacgcattga aacgctgatg cgcaacctga
     7501 tgccgtggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt
     7561 ccgactggaa atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc
     7621 tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc
     7681 tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac
     7741 tactgggtaa tgatcagcgc gcgcatttgt taccgttagg tattgaacaa cttccggcac
     7801 tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta tcatcgtcgt tcaccgctgg
     7861 agcatctctg gcagttgaaa gatcaactgg tcaatgaagg tgaactggtg cttgaaacgc
     7921 tggttattga tggcgatgag aacacggtgc tggtgccggg cgatcgttac gcgcaaatgc
     7981 gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt
     8041 ttgtagatat ccgcattgcg gatgtgagcg ttaccactac agaagagcag cgacgcactg
     8101 aatggatggt taccgagtct ctgtccgatt tcctcgaccc acatgatccg agtaagacag
     8161 ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg
     8221 gtattactgc cggatgcggc gtgaacgcct tatccagcct acaaaacctt gctaattcaa
     8281 tatattgcag ggactatgta ggcctgataa gcatagcgca tcaggcagct ttatgtttgc
     8341 ataacctcag cgcccgtttc cgggcgcttt tcacgtctta tgcctgaaca gctggctgat
     8401 caaacgccgt taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt
     8461 taccggcaca ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac
     8521 tgccgttttt acacaagcca ttttccagat ctggccatgc accttcatgc acgcatacca
     8581 cgcctttttt gatcccgtca gtcaccacag cgcctgtcaa aatctgacca cgtttgttcc
     8641 agacacggac cagatcgcca ttcgcaatac caaagcgagc agcatcttcg gtgtgaatag
     8701 tgattggttc acgatcggca accgcatatt ttttacgcag ttgcgcatag ttaagctgac
     8761 tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgtaa ctgcttctcg tcggcggtac
     8821 ctttccactc atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt
     8881 tttccagcgt tttggagtaa atctcaattt tgccgcttgg cgtacccagc gcatttttca
     8941 ccggatcggc gcggaaatca ccataacgaa cgtactgttc gttcttctcg ctgcggcgca
     9001 tttcgatcag tttattttgc tgccagaagg cattaaacat tggcatagtg acgcgttgcg
     9061 cacgggcacc tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac
     9121 cttcggtata gatctctttt ccgcctggtt tcagtaattc cgcaagttcg gcaaacacgt
     9181 caaaatcgtt tcgcgcttca aattgcggag cgacagcctg cttcatcggt acaatatgct
     9241 ggttgctgta atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta
     9301 atacgatatc tgcatgttta gcagctgcgg tccagtagca ttcagaaacg acgatcatct
     9361 ccggtttctg ccatgctttg atcagacgat tagtatcctg gtgatgggta aagttaccgc
     9421 cacccgccca ccagatcatt ttgatattcg gatacgtttg ttctttaccg ttatgctgat
     9481 atttgccgcc aggattttcc agcgcatcga cgatacgggc aacaggaatc gccgtcattc
     9541 cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca
     9601 cgccgccaac acgcgtcggg ttgccaccgt tggagtaatg ataagagaaa ccaaaaccgc
     9661 cgcccggtgt accaatctgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct
     9721 gttcaccata ttgctggcgc tgaattcccc agcctgccat cagcattgtg cgattcgccg
     9781 ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgtcc
     9841 aggccgcgct cttcggagta ttatcgctct tacctgtcag atactcttca aactgcggat
     9901 acccggtggt gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat
     9961 gcgcaatccc taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat
    10021 tatagccaaa gaactcgatg gtttcagagc ggataggatc aatggcaatc actggtttgc
    10081 cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa
    10141 tttttaaggt attcagcggg ttcattcccc acagcaccac aacctggctg ttttccagaa
    10201 tcagcggcca ggaggtctgc tgttcataca cctctacaga accgaccaca tgcggcatga
    10261 tcacctgtgc tgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt
    10321 tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt
    10381 aagatccggc aaaaatggcc gatggtccgt tagctttacg aatacgctca tgttgctcat
    10441 gaatcagttt taatgcctgt tcccagctca cctgtacata ggtatcttcg ccacgacctt
    10501 tcgccggttg cagtggattg tcgagatagc tttttctcac catcggatgc tgaatacgcg
    10561 ccgtggtgtg tacctgatcc gccgccgtag actgtaagga gttcggtatg gttttcgcca
    10621 gcgcgcctgt cgaagaaaca atcttgccgt ccttcacttc tacgttcatc gctccccaac
    10681 gtcccgcggt gaggatttta ccgcccttct cttctgccca tgcgggtagc ggtgctgccg
    10741 atgtcaccac cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtcttgtta
    10801 atgtcataac ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt
    10861 tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgtgccc
    10921 ccattccttt ggcaatggac ggccacgcat tgacggtgta atggtcggcg gcaatagggg
    10981 catgacaacc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcagtttac
    11041 ggtccgctaa tgcgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc
    11101 cgtattcgtc agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta
    11161 atgtggctag cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca
    11221 tttgataacc ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat
    11281 cggtaccagg attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat
    11341 gtgcgccatt agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat
    11401 ccatttttat ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc
    11461 cgcccttttg tgctttgtta tgcattttct gcgcagattc actttgcgag gcaatatcca
    11521 tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc
    11581 atactgtttc ggccatttcc tggcgatgag cttcgaactt atcgtcactg tctattttg
//
LOCUS       DBFIFS010000140        11621 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.140, whole genome shotgun sequence.
ACCESSION   DBFIFS010000140 DBFIFS010000000
VERSION     DBFIFS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.140"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1237)
                     /gene="gltA"
                     /locus_tag="N7A07_003291"
     CDS             complement(<1..1237)
                     /gene="gltA"
                     /locus_tag="N7A07_003291"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU2477897.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQL"
     gene            complement(1443..1550)
                     /locus_tag="N7A07_003292"
     CDS             complement(1443..1550)
                     /locus_tag="N7A07_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477898.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            1931..2335
                     /gene="sdhC"
                     /locus_tag="N7A07_003293"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             1931..2335
                     /gene="sdhC"
                     /locus_tag="N7A07_003293"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU2477899.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2329..2676
                     /gene="sdhD"
                     /locus_tag="N7A07_003294"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2329..2676
                     /gene="sdhD"
                     /locus_tag="N7A07_003294"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU2477900.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2676..4442
                     /gene="sdhA"
                     /locus_tag="N7A07_003295"
     CDS             2676..4442
                     /gene="sdhA"
                     /locus_tag="N7A07_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU2477901.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4458..5174
                     /gene="sdhB"
                     /locus_tag="N7A07_003296"
     CDS             4458..5174
                     /gene="sdhB"
                     /locus_tag="N7A07_003296"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU2477902.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5475..8276
                     /gene="sucA"
                     /locus_tag="N7A07_003297"
     CDS             5475..8276
                     /gene="sucA"
                     /locus_tag="N7A07_003297"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU2477903.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8291..9508
                     /gene="odhB"
                     /locus_tag="N7A07_003298"
     CDS             8291..9508
                     /gene="odhB"
                     /locus_tag="N7A07_003298"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU2477904.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9602..10768
                     /gene="sucC"
                     /locus_tag="N7A07_003299"
     CDS             9602..10768
                     /gene="sucC"
                     /locus_tag="N7A07_003299"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU2477905.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10768..>11621
                     /gene="sucD"
                     /locus_tag="N7A07_003300"
     CDS             10768..>11621
                     /gene="sucD"
                     /locus_tag="N7A07_003300"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU2477906.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEAL"
BASE COUNT         2774 a         2983 c         3222 g         2642 t
ORIGIN      
        1 acaactggcg cggacgggcg atcttcatgc cgtcactgtg catttcgctc cagtgagcga
       61 tccagccaac ggtacgggcc atagcgaaga tgacggtgaa catggaagac ggaataccca
      121 tcgctttcag gatgatacca gagtagaaat cgacgttcgg gtacagtttc ttctcgataa
      181 agtacgggtc gttcagcgcg atgttttcca gctccatagc cacttccagc aggtcatctt
      241 tggtgcccag ctctttcaga acttcatggc aggtttcacg cattacggtg gcgcgcgggt
      301 cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca tttttatctt
      361 tcgcacgacg aacaaattcc ggaatgtgtt taacggagct aatttcttcc agcattttca
      421 gcgccgcttc gttagcacca ccgtgtgcag gcccccacag tgaagcaata cctgctgcga
      481 tacaggcaaa cgggttcgca cccgaagagc cagcggtacg cacggtggag gtagaggcgt
      541 tctgttcatg gtcagcgtgc aggatcagaa tacggtccat agcacgttcc agaatcggat
      601 taacttcata cggttcgcac ggcgtggaga acatcatatt caggaagtta ccggcatagg
      661 agagatcgtt gcgcggataa acaaatggct gaccaatgga atacttgtaa cacatcgctg
      721 ccatggtcgg cattttcgac agcaggcgga acgcggcaat ttcacgatga cgaggattgt
      781 taacatccag cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca cacatgactg
      841 ccattggatg tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc tcgtggatca
      901 tggtatgacg ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc ggtttttcac
      961 cattcagcag gatgtaacaa acttccaggt agttagaatc ggtcgccagc tgatcgatcg
     1021 ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat aaaagtaatt ttagattcgc
     1081 aggatgcggt tgaagtgaag ccagggtcaa aggtgaacac accttttgaa ccgagagtac
     1141 ggatatcaat aacatcttga cccagcgtgc ctttcagcac atccagttca acagctgtgt
     1201 ccccgttgag ggtgagtttt gcttttgtat cagccattta aggtctcctt agcgccttat
     1261 tgcgtaagac tgccggaact taaatttgcc ttcgcacatc aacctggctt tacccgtttg
     1321 ttatttggct cgccgctctg tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt
     1381 gcaggtaaag gatccattga tgacgaataa atggcgaatc aagtacttag caatccgaat
     1441 tattaaactt gtctaccact aataactgtc ccgaatgaat tggtcaatac ttccacactg
     1501 ttacataagt taatcttagg tgaaataccg acttcataac ttttacgcat tatatgcttt
     1561 tcctggtaat gtttgtaaca actttgttga atgattgtca aattagatga ttaaaaatta
     1621 aataaatgtt gttatcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg
     1681 taggttaatt gtaatgattt tgtgaacggc ctatactgcc gccaggtctc cggaacaccc
     1741 tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt
     1801 gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa
     1861 atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa
     1921 taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa
     1981 tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt
     2041 ttccggtgtg atcacctttg ttgcagtggg tatcctgctg tggcttctgg gtaccagcct
     2101 ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt
     2161 tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat
     2221 gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat
     2281 ctcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac
     2341 gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc
     2401 gtcctgacgc tctacatcat ttatatggtc ggttttttcg ccaccagtgg cgagctgaca
     2461 tatgaagtct ggatcggttt ctgcgcctct gcgttcacca aagtgttcac cctgctggcg
     2521 ctgttttcta tcttgatcca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt
     2581 aaaccgctgg ctttgcgcct gatgctgcaa ctggtgattg tcgttgcact ggtggtttac
     2641 gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg
     2701 atgcagttgt gattggtgcc ggcggcgcag gtatgcgcgc ggcgctgcaa atttcccaga
     2761 gcggccagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg
     2821 cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata
     2881 tgtacgacac cgtgaaaggg tcggactata tcggtgacca ggacgcgatt gaatatatgt
     2941 gtaaaaccgg gccggaagcg attctggaac tcgaacacat gggcctgccg ttctcgcgtc
     3001 tcgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg
     3061 agcaggcggc acgcactgcg gcagcggctg accgtaccgg tcacgcactg ttgcacacgc
     3121 tttatcagca gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc
     3181 tggtgaaaaa ccaggatggc gcggtggtgg gttgtaccgc actgtgcatc gaaaccggtg
     3241 aagtggttta ttttaaagcc cgcgctaccg tgctggcgac tggcggagca gggcgtattt
     3301 atcagtctac cactaacgcc cacattaaca ccggcgacgg cgtcggcatg gctatccgcg
     3361 caggcgtacc ggtgcaggat atggaaatgt ggcaattcca cccgaccggc attgccggtg
     3421 cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg
     3481 gcgaacgctt tatggagcgt tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg
     3541 tggcgcgttc aatcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc
     3601 cacacgcgaa actgaaactc gatcacctgg gtaaagaagt tctcgaatcc cgtctgccgg
     3661 gtatcctgga gctttcccgt accttcgctc acgttgatcc ggtgaaagag ccgattccgg
     3721 ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagttacc ggtcaggcgc
     3781 tgactgtgaa tgagaaaggc gaagatgtgg ttgttccggg gctgttcgcg gtaggtgaaa
     3841 ttgcctgcgt atcggtacac ggtgctaacc gtctgggcgg caactcgctg ctggacctgg
     3901 tggtctttgg tcgcgcagcg ggtctgcatc tgcaagagtc tatcgccgag cagggcgcat
     3961 tgcgcgatgc cagcgagtct gatgttgaag catctttgga tcgtctgaac cgctggaaca
     4021 ataatcgtaa cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc
     4081 ataacttctc ggtcttccgt gaaggtgatg cgatggctaa agggcttgag cagttgaaag
     4141 tgatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag ttcaacaccc
     4201 agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt
     4261 ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc ccggatcgtg
     4321 atgatgaaaa ctggctgtgc cactccctgt atctgccaga gtcggaatcc atgacgcgcc
     4381 gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact
     4441 aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg
     4501 atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgc gacatgatgc
     4561 tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgccgctcct
     4621 gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct
     4681 gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc
     4741 caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg
     4801 agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac
     4861 agatgccaga gcagcgcgaa aaactcgacg ggctgtatga atgtattctc tgcgcatgtt
     4921 gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct
     4981 tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg
     5041 acggtttgag cgatgcattc agtgtattcc gctgtcatag catcatgaac tgcgtcagtg
     5101 tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc
     5161 aacgtaatgc gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt
     5221 gccggatgcg gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag
     5281 cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact
     5341 aagacagttt ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac
     5401 cccggcacgc acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg
     5461 cttaagggat cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct
     5521 ctggcgcaaa ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact
     5581 cggttgacgc taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg
     5641 atcaattcca ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt
     5701 actcttcaac gatctccgac cctgacacca atgtgaagca ggtaaaagtc ctgcagctca
     5761 ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc
     5821 agcaagataa agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc
     5881 aggagacctt caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc
     5941 tgctggaagc cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatta
     6001 ccagcaccga agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca
     6061 atagcgaaga gaaaaaacgc ttcctaagcg aactgaccgc cgctgaaggc cttgaacgtt
     6121 acctcggtgc aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa
     6181 tcccgatgct taaagagatg attcgccacg ctggcaacag cggcacccgc gaagtggttc
     6241 tcgggatggc gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc
     6301 aagacttgtt cgacgagttt gccggtaaac ataaagaaca cctcggcacg ggtgacgtga
     6361 aataccacat gggcttctcg tctgacttcc agaccgatgg cggcctggta catctggcgc
     6421 tggcgtttaa cccgtctcac cttgagattg tcagcccggt cgttatcggt tctgttcgtg
     6481 cccgtctgga cagacttgat gagccgagca gcaacaaagt gctgccaatc accattcacg
     6541 gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac tctgaacatg tcgaaagcgc
     6601 gtggttatga agttggcggt acggtacgta tcgttatcaa caaccaggtt ggcttcacca
     6661 cctctaaccc gctggatgcc cgttcaacgc cgtactgtac tgatatcggt aagatggttc
     6721 aggcaccgat ttttcacgtt aacgcggatg atccggaagc cgttgccttt gtgacccgtc
     6781 tggcgctcga tttccgtaac acctttaaac gtgatgtctt catcgacctg gtgtgctacc
     6841 gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga
     6901 aaatcaaaaa acatccgacg ccgcgcaaaa tctatgctga caagctggag caggaaaaag
     6961 tggcgacgct ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg
     7021 gcgattgcgt agtggcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt
     7081 acctcaacca cgaatgggac gaagagtacc cgaataaagt tgagatgaag cgcctgcagg
     7141 agctggcgaa acgcatcagc acggtgccgg aagcggttga aatgcagtct cgcgttgcca
     7201 aaatttatgg cgatcgccag gcgatggctg ccggtgagaa actgttcgac tggggcggcg
     7261 cggaaaacct cgcttacgcc acgctggttg acgaaggcat tccggttcgc ctgtcgggtg
     7321 aagactccgg tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg
     7381 gttccactta cacgccgctg caacacatcc ataacggcca gggcgcgttc cgtgtctggg
     7441 actccgtact ttctgaagaa gcagtgctgg cgtttgaata cggttatgcc accgcagaac
     7501 cacgcactct gactatctgg gaagctcagt tcggtgactt cgccaacggt gcgcaggtgg
     7561 ttatcgacca gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggtctggtga
     7621 tgttgctgcc gcacggttac gaagggcagg ggccggagca ctcctccgcg cgtctggaac
     7681 gttatctgca actttgtgct gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac
     7741 aggtttacca catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga
     7801 tgtcgccgaa atccctgctg cgtcatccgc tggcggtttc cagcctcgaa gaactggcga
     7861 acggcacctt cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc
     7921 gcgtagtgat gtgttctggt aaggtttatt acgacctgct ggaacagcgt cgtaagaaca
     7981 atcaacacga tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga
     8041 tgcaggaagt gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc
     8101 cgctcaatca gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg
     8161 gggcttctct gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt
     8221 ccgttcacca gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa
     8281 aggatacaca atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga
     8341 tgccaccgtc gcaacctggc ataaaaaacc cggtgacgca gtcgtacgtg atgaagtgct
     8401 ggtagaaatc gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct
     8461 ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc ttggtcgcct
     8521 gcgtgaaggc aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac
     8581 tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc
     8641 gatccgtcgc ctgctggctg aacataacct cgacgccagc gccattaaag gcaccggtgt
     8701 gggtggtcgt ctgacccgtg aagatgtgga aaaacatctg gcgaaagctc cggcgaaaga
     8761 gtctgcaccg gcagcggctg ctccggcggc gcaaccggct ctggctgcac gtagcgaaaa
     8821 acgtgtcccg atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa
     8881 ctccaccgcc atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg
     8941 taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt
     9001 ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaatg cttctatcga
     9061 cggcgatgac gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg
     9121 cggcctggtg acgccggtac tgcgtgatgt cgataccctc ggcatggcag acatcgagaa
     9181 gaaaatcaaa gagctggcag ttaaaggccg tgacggcaag ctgacggttg aagatctgac
     9241 cggtggtaac ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat
     9301 catcaacccg ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc
     9361 tgtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg
     9421 tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga
     9481 tccgacgcgt ctgctgctgg acgtgtagta gtttaagttt cacctgcact gtagaccgac
     9541 gtggataggg tgtcggtcta cggtttaaaa gataacgatt actgaaggat ggacagaaca
     9601 catgaactta catgaatatc aggcaaaaca actgtttgcc cgctatggct taccagcacc
     9661 ggtgggttat gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa aaatcggtgc
     9721 cggtccgtgg gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag cgggcggtgt
     9781 gaaagttgta aacagcaaag aagacatccg tgcttttgca gaaaactggc tgggcaagcg
     9841 tctggtaacg tatcaaacag atgccaatgg ccaaccggtt aaccagattc tggttgaagc
     9901 agcgaccgat atcgctaaag agctgtatct cggtgccgtt gtagaccgta gttcacgtcg
     9961 cgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg cggaagaaac
    10021 tccgcacctg atccacaaag ttgcgcttga tccgctgact ggcccgatgc cgtatcaggg
    10081 acgcgagctg gcgttcaaac tgggtctgga aggtaaactg gttcagcagt tcaccaaaat
    10141 cttcatgggc ctggcgacca ttttcctgga gcgcgacctg gcgctgatcg aaatcaaccc
    10201 gctggtcatc accaaacagg gcgatctgat ttgcctcgac ggcaaactgg gcgctgatgg
    10261 caacgccctg ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc aggaagatcc
    10321 gcgtgaagca caggctgcac agtgggaact gaactacgtt gcgctggacg gtaacatcgg
    10381 ttgtatggtt aacggcgcag gcctggcgat gggtacgatg gacatcgtta aactgcacgg
    10441 cggcgaaccg gctaacttcc tggacgttgg cggcggcgca accaaagaac gtgtaaccga
    10501 agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc gttctggtta acatcttcgg
    10561 cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc ggtgcggtag cggaagtggg
    10621 tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg gcgcgaagaa
    10681 actggctgac agcggcctga atattattgc agcaaaaggt ctgacggatg cagctcagca
    10741 ggttgttgcc gcagtggagg ggaaataatg tccattttaa ttgataaaaa caccaaggtt
    10801 atctgccagg gctttaccgg tagccagggg actttccact cagaacaggc cattgcatac
    10861 ggcactaaaa tggttggcgg cgtaacccca ggtaaaggcg gtactaccca cctcggcctg
    10921 ccggtgttca acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc ttctgttatc
    10981 tacgtaccag caccgttctg caaagactcc attctggaag ccatcgacgc aggcatcaaa
    11041 ctgattatca ccatcactga aggcatcccg acgctggata tgctgaccgt gaaagtgaag
    11101 ctggatgaag caggcgttcg tatgatcggc ccgaactgcc caggcgttat cactccgggt
    11161 gaatgcaaaa tcggtattca gcctggtcac attcacaaac cgggtaaagt gggtatcgtt
    11221 tcccgttccg gtacactgac ctatgaagcg gttaaacaga ccacggatta cggtttcggt
    11281 cagtcgacct gtgtcggtat cggcggtgac ccgatccctg gctctaactt tatcgacatt
    11341 ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg tgagatcggc
    11401 ggtagcgctg aagaagaagc agctgcgtac atcaaagagc acgttaccaa gccagttgtg
    11461 ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc gggtgccatc
    11521 attgccggtg ggaaagggac tgcggatgag aaattcgctg ctctggaagc cgcaggcgtg
    11581 aaaaccgttc gcagcctggc ggatatcggt gaagcactga a
//
LOCUS       DBFIFS010000141        11608 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.141, whole genome shotgun sequence.
ACCESSION   DBFIFS010000141 DBFIFS010000000
VERSION     DBFIFS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.141"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1344)
                     /gene="uidB"
                     /locus_tag="N7A07_003301"
     CDS             complement(<1..1344)
                     /gene="uidB"
                     /locus_tag="N7A07_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU2477907.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQ"
     gene            complement(1341..3154)
                     /gene="uidA"
                     /locus_tag="N7A07_003302"
                     /pseudo
     CDS             complement(1341..3154)
                     /gene="uidA"
                     /locus_tag="N7A07_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(3545..4135)
                     /gene="uidR"
                     /locus_tag="N7A07_003303"
     CDS             complement(3545..4135)
                     /gene="uidR"
                     /locus_tag="N7A07_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU2477908.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4364..5131)
                     /gene="hdhA"
                     /locus_tag="N7A07_003304"
     CDS             complement(4364..5131)
                     /gene="hdhA"
                     /locus_tag="N7A07_003304"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU2477909.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5243..6271)
                     /gene="malI"
                     /locus_tag="N7A07_003305"
     CDS             complement(5243..6271)
                     /gene="malI"
                     /locus_tag="N7A07_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU2477910.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLVHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6446..8038
                     /gene="malX"
                     /locus_tag="N7A07_003306"
     CDS             6446..8038
                     /gene="malX"
                     /locus_tag="N7A07_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU2477911.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8048..9220
                     /gene="malY"
                     /locus_tag="N7A07_003307"
     CDS             8048..9220
                     /gene="malY"
                     /locus_tag="N7A07_003307"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU2477912.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9324..10325
                     /gene="add"
                     /locus_tag="N7A07_003308"
     CDS             9324..10325
                     /gene="add"
                     /locus_tag="N7A07_003308"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU2477913.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(10361..11401)
                     /locus_tag="N7A07_003309"
     CDS             complement(10361..11401)
                     /locus_tag="N7A07_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU2477914.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAIMLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
BASE COUNT         2812 a         3077 c         2845 g         2874 t
ORIGIN      
        1 ctgcactttt ttacgattat caatttcaac cacgatttct ttgaattttt tatccgtgag
       61 cggataaaac cagataataa cgaatgccag tagcataaat ccgcaaggta ctaaggcaat
      121 tgatgtgcgg atgcccataa ttacttccgg cgtttgcacc tgattggcga tatatccgct
      181 taatcccaaa ataaaggcag gaattgaacc accgattgcc tgaccgcatt tacgggtaaa
      241 tgagaatagt gaataggtga gcccttcaat tcgcacgccg gtcaggtatt caccgtattc
      301 tacggtatca gcttccagcg cccacatcac ggtcatggta acgccctgac caattgaggc
      361 aatggctaac gcaaccaacg ccaccggcag cgaccagacg gaaacccaga agaacagcag
      421 ataaccgcag gttcccagca aagcgccaat caggaaggta ttctttttac cgatcctcgc
      481 gaccatcccc ggcaccaacg gtgccgatgc cacagtacca accaggtttt gcaccagtac
      541 cagcacagtg aacagcccgg tatcatttaa cacatagcgc acgtagaaca acgacgaggc
      601 gctgaccgcg aaggtcgaaa tcagcacaca cagcgcaccg atgcacaaca taaacagcgg
      661 gcgattccgt ttcagggttt gcagactgat cttcaatgac ggctgcgcca cgatacgtac
      721 cacattctca cgcgtcgatt tgaagcagat gaagtaaagc accattccgg caatcgccag
      781 cacaatcgtc cagaaatggt ataccgacac catctcttcc gggctggagt tcttaatgct
      841 cggccctatc agaaatgcca ggcagacaaa ggtcaatgaa gcggcaatcc cacgagccgc
      901 gcccagacgg gcgcgggatt gtggttgttg ggtcatcgcg gtagcaagtg aaccataagg
      961 aatattcacc aggctgtagc aaagcccgag gcccatgtag gtcaaatatg catacaccac
     1021 tttgctacca tggctccagt cggtcggcac ccagaatacc agcacgctga agatcattaa
     1081 cggcgcagta ccgaagagta aaaacgggcg gaattttccc cagcgggtat tcacactgtc
     1141 caccactcgt ccggcaaaga cgtcggcgaa ggcatcgaat acccgcacca gtaacagcat
     1201 ggtgcccgcc gcagcggcac cgacgccagc gacgtcggtg tagtaactca acaggaagag
     1261 cgcccccatt gcgaaggcga agttattggc gatgtcacca aggctgtagc cgacgatggt
     1321 gcgccaggag agttgttgat tcattgtttg cctccctgct gcggtttttc accgaagttc
     1381 atgccagtcc agcgtttttg cagcagaaaa gccgccgact tcggtttgcg gtcgcgggtg
     1441 aagatcccct tcttgttacc gccaacgcgc aatatgcctt gcgaggtcgc aaaatcggcg
     1501 aaattccata cctgttcacc gacgacggcg ctgacgcgat caaagacgcg gtgatacata
     1561 tccagccatg cacactgata ctcttcactc cacatgtcgg tgtacattga gtgcagcccg
     1621 gctaacgtat ccacgccgta ttcggtgatg ataatcggct gatgcagttt ctcctgccag
     1681 gccagaagtt ctttttccag taccttctct gccgtttcca aatcgccgct ttggacatac
     1741 caaccgtaat aacggttcag gcacagcaca tcaaagagat cgctgatggt atcggtgtga
     1801 gcgtcgcaga acattacatt gacgcaggtg atcggacgcg tcggatcgag tttacgcgtt
     1861 gcttccgcca gtggcgcgaa atattcccgt gcaccttgcg gacgggtatc cggttcgttg
     1921 gcaatactcc acatcaccac gcttgggtgg tttttgtcac gcgctatcag ctctttaatc
     1981 gcctgtaagt gcgcctgctg agtttccccg ttgactgcct cttcgctgta cagttctttc
     2041 ggcttgttgc ccgcttcgaa accaatgcct aaagagaggt taaagccgac agctgcagtt
     2101 tcatcaatca ccacgatgcc atgttcatct gcccagtcga gcatctcttc agcgtaaggg
     2161 taatgcgagg tacggtagga gttggcccca atccagtcca ttaatgcgtg atcgtgcacc
     2221 atcagcacgt tatcgaatcc tttgccgcgc aaatccgcat cttcatgacg gccaaagcca
     2281 gtaaagtaga acggtttgtg gttgatcagg aactgttcgc ccttcactgc cactgaccgg
     2341 atgccgacgc gcagcgggta gatatcacac tctgtccggc ttttggctgt gacgtacagt
     2401 tcatagagat aaccttcacc cggttgccag aggtgcggat tcaccacttg caaagtcccg
     2461 ctggtgcccc ttgtccagtt gcaaccacct gttgatccgc atcacgcagt tcaacgctga
     2521 catcaccatt ggccaccacc tgccagtcaa cagacgcgtg gttacagtct tgcgcgacat
     2581 gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc attacgctgc
     2641 gatggatccc ggcgtagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt
     2701 cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa
     2761 ctgtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg
     2821 tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag
     2881 tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt ataaagactt
     2941 cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg aactgatcgt
     3001 taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct
     3061 gctcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga
     3121 tttcacgggt tggggtttct acaggacgta acataaggga ctcctcatta agataaaaat
     3181 actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag ttatctgtga
     3241 gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa aaaaccaaaa
     3301 actgcaatac ccacagcgtg atatagatcg cattaatctt taaaacggcg ttgacagaag
     3361 ccataaaata agcaaccgaa cgttcgttcg agcatgttgg gaacgataaa ctctcatgac
     3421 atatggctac ggtgaatatt ttagggagga attccccgct ccggcgagat tgtattttgt
     3481 acagggaaat taaagtatga aacgcgggct cagttgtcac cagcccgcgg aaagagagag
     3541 agaatcagga tgcggttaag ataccgccaa tcatcgcgcg taatccctga gcaaacgacg
     3601 cttcacgcgc caggtcttcc gcttcgatat cggccagtac accgtaggtc agatccagca
     3661 gtaaacgtga agtcattgcc gtgttgatgt ctgggcttat ctcgcctttt tgctgggcat
     3721 cacgcatccg ctgagcaaca aattcagtga tcgtcatatg cttattttta agcatggcgg
     3781 cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc cgttgcccaa
     3841 aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa tgaatccctt
     3901 caatcggttc ccggaaacgg gccagcgccc tctcctggtc ctgtaagata atggcctgaa
     3961 tcaaggcttc tttggagatg aaatggtgat agagcgtccc gggactaatg gcgcaggatt
     4021 tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc tctctggcag
     4081 cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc atcattaaac
     4141 cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc tatcattgct
     4201 gttgaatagt gcgaaggcac actctattcg tatgcttttc atccagctat tggttaacca
     4261 atggccgtga tttggctgcc agttgtttag cagagacgtt gatgaattgc acacgctatc
     4321 ccggtaaaca acacatacgg ctgtttaccg gttcgttagt gtattaattg agctcctgta
     4381 ccccaccacc ggagacggtg agaatttgtc cgcttaccca gctggctgca ggcgagcaaa
     4441 ggaacagcgc tgcgttagca atatcttgcg gttggcccag acgtctgatc ggcgtgtgct
     4501 gtaacatttt ttgttcaatt tctggtgtaa taacggattt cagggcatcg gttaatattg
     4561 cccccggcgc aatgccattc acccgaatat ttttttcacc caggtcaaat gccatatttc
     4621 tgaccagatg actggccgca gctttagatg atgcatagga agtcatgttt atatttttat
     4681 tttctgccgc catagaagtg atggtcagaa taacgccacc gccatttttt tccatttctg
     4741 gcgcaacaag ttgtgacaga tggaaaaaag aaaacacatt cagttcataa gcacggcgaa
     4801 aatccgccat tggcatatca aacggtttag gtccaccgcc accggcgttg ttaaccagaa
     4861 tatcaacttt acccagctta ctgatagcaa agtctgccag tgcagagagt tcctgttcgg
     4921 aagtaatatc acaacggcag gcaaatgcct gaccacccag ttgttgaatt tcgtctacaa
     4981 catggttagc tgcgtcggcg ttaatatcac tgaccaccac agatgcgcca gctgtcgcga
     5041 atgtaatggc gatttcttta ccaatacctg cacccgcacc tgtgatgatg gcgcattttc
     5101 cgtcgagtct caggttgtca gaattaaaca cataaacctc ctgtgaactt cagtaaaaat
     5161 aaaaggcacc gcctgtgtgc cacatttttt atcattaaac ttgaggaaag gacactacca
     5221 ggtatagcag ataaagagag gattatttcg ccgcaatgag ccgggcggga ataataagat
     5281 tgcgtgaatg cgtctcttca tgggtgattt tttgcatcat gcgatccgca agtgtggtac
     5341 caagttcccg cgcaggcgtg ctggcccagg taacgggtat atcatcaagt gtggttggtg
     5401 tcgcatcggt aaatgccgcc agcgaaacct gttgctcaaa gtaacgatcg actccgcttt
     5461 ccccgctttg ccgccctgct ttcagcaaac caaaccatgc ccccatcgca atagtttcgt
     5521 tatagcaaac cacggcactg atggtcgggt tatgacgtaa aagcgccgtg atagcttccg
     5581 cggcttgctt ctggctggaa gtgcactcca acacccaatc gctgtgaaac ggcaggccaa
     5641 attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat gaggaacttt
     5701 gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc gtcaacaact
     5761 gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa ctggcacggg
     5821 aagcgaaaat caccgggata gctttttctt ctgccatccg tcgcaggtca tcgctgcttc
     5881 ctgcagcccc ggcaattacc acaccatcga caccctgatt cagtaacagt gaaaaccgct
     5941 gtgccagctg ctcgccgtct ttaccgccgt gaaccaaaaa aaccatccgt ccctgcgctt
     6001 ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga
     6061 cgatcaaacc aatgacgccg ctttgcccac cgcgcagcgc cgacgcctgg cgattgcgca
     6121 caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc
     6181 ctttgccact aagcaccagc gaaacggtac ttaccgacac gcccgcagcc agcgcaacat
     6241 catgaatggt tatttttttg gcggtagcca tgcagatgac ctactccctg attttgtgac
     6301 agataaaacg ttttaccttt tatttatctt atacccgcta ttatcgttgc gtaatgtgat
     6361 ttatgcctca ctaaaatttg ataaaacgtt ttatcttctc gcgcaattta ctgaatccag
     6421 attgttctct acgaggagtc gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg
     6481 ggagttcttc cagcagttag gcaaaacctt catgttaccc gtggcattat tgtcgttctg
     6541 cggcattatg ctcggcattg gtagttccct tagtagccac gatgtcatca ccctgctccc
     6601 ggtcctgggc aaccccgtgt tgcaggctat ctttacctgg atgagtaaga ttggctcgtt
     6661 tgcttttagt ttcctgccag tcatgttctg tatcgccatc ccgctgggtc tggcacgaga
     6721 aaacaaaggc gtagcggcat tcgctggctt cgtcggttat gcggtaatga acctcgcggt
     6781 aaacttctgg ttgaccaata aaggcattct gccaaccacg gatgccgccg ttctgaaagc
     6841 caataacatc cagagcattc ttgggatcca gtcgatcgac accgggatcc tcggtgcggt
     6901 gatcgccggt attatcgtct ggatgctgca tgagcgattc cataatatcc gcctgccgga
     6961 tgcgctggca ttcttcggcg gtacgcgctt cgtaccaatc atctcctcgc tggtgatggg
     7021 ccttgtcggc ctggtgattc cattagtctg gccgattttc gccatgggta ttagcggctt
     7081 aggccatatg atcaatagcg cgggtgattt cggaccgatg ctgtttggta ccggtgaacg
     7141 tctgctgttg ccgtttggtc tgcatcacat tctggtggca ttaattcgct ttaccgacgc
     7201 aggtggcacg caggaagtct gcggtcaaac cgtcagcggc gcattgacca tcttccaggc
     7261 gcaattgagt tgcccgacca ctcacggttt ttctgaaagc gccacgcgtt tcctttcgca
     7321 aggcaaaatg cctgcgtttc tcggcggtct gccaggtgca gcgttagcga tgtatcactg
     7381 cgcgcgcccg gaaaatcgcc ataaaattaa agggctgctg atttctggcc tgatcgcctg
     7441 tgtcgttggc ggcacgaccg aaccgctgga attcctgttc ctgttcgtag cgccagttct
     7501 gtatgtcatc cacgcgctgt taaccggcct cggcttcacc gtcatgtctg tgctcggcgt
     7561 caccatcggt aataccgacg gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg
     7621 tctgtcaacc aagtggtaca tggtgccagt ggtggtggca atctggtttg tcgtttacta
     7681 cgtcatcttc cgtttcgcta tcacccgctt caatctgaaa accccggggc gcgatagcga
     7741 agtggccagt tcaatcgaaa aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt
     7801 tccggcaatc ctcgaagcct taggcggtgc cgacaatatt gttagcctcg ataactgcat
     7861 tacccgtctg cgtttgtctg tgaaagatat gtcgcttgtt aatgtgcagg cactgaagga
     7921 caatcgggca attggcgtag tacaacttaa tcaacataac ctgcaggttg ttatcgggcc
     7981 acaagttcag tcagtaaaag atgaaatggc cggtctgatg catactgtcc aggcataagg
     8041 ataagatatg ttcgattttt caaaggtcgt ggatcgtcat ggcacatggt gtacgcagtg
     8101 ggattatgtc gctgaccgtt tcggcactgc tgacctgtta cctttcacga tttcagacat
     8161 ggattttgcc actgccccct gcattatcga ggcgctgaat cagcgcctga tgcacggcgt
     8221 gtttggctac agccgctgga agaacgatga gtttctcgcg gctattgccc actggttttc
     8281 cacccagcat tacaccgcca tcgatcctca gtcggtggtg tatggccctt ctgttatcta
     8341 tatggtctca gaactgattc gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac
     8401 acccgcctat gacgcatttt acaaggccat tgaaggtaat cagcgcacag taatgcccgt
     8461 tgctttagag aagcaggctg acggctggtt ttgcgatatg ggcaagctgg aagccgtgtt
     8521 ggcgaaacca gaatgtaaaa ttatgctcct gtgtagccca cagaatccta ccggaaaagt
     8581 gtggacgtgc gatgagctgg agatcatggc tgacctgtgc gagcgtcatg gtgtgcgggt
     8641 tatttccgat gaaatccata tggatatggt ttggggcgag cagccgcata ttccctggag
     8701 taatgtggcg cggggagact gggcgttgct gacgtcggga tcgaaaagtt tcaatattcc
     8761 cgcgctgacc ggtgcttacg ggattataga aaatagcagc agccgtgatg cctatttatc
     8821 ggcactgaaa ggacgtgatg ggctttcttc cccttcggta ctggcgttaa ctgcccatat
     8881 cgccgcctat cagcaaggtg caccgtggct ggatgcctta cgcgtctatc tgaaagataa
     8941 cctgacgtat atcgcagata aaatgaacgc cgcgtttcct gaactcaact ggcagatccc
     9001 gcaatccact tatctggcct ggcttgattt acgtccgttg aatattgacg acaacgcgtt
     9061 gcaaaaagca cttatcgaac aagaaaaagt cgcgatcatg ccggggtata cctacggtga
     9121 agaaggtcgt ggttttgtcc gactcaatgc cggctgccca cgttcgaaac tggaaaaagg
     9181 tgtggctgga ttaattaacg ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa
     9241 aaatcgttgc gcaatcgtgg atttttaccc tgctttgttt ttataatggt gcgcactttt
     9301 atatccagaa aaagagtgcg accatgattg ataccaccct gccattaact gatatccatc
     9361 gccaccttga tggcaacatt cgtccccaga ccattcttga acttggccgc cagtataata
     9421 tctcgcttcc tgcacaatcc ctggaaacac tgattcccca cgttcaggtc attgccaacg
     9481 aacccgatct ggtgagcttt ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc
     9541 ttgatgcttg tcgccgcgtg gcatttgaaa acattgaaga tgcagcccgt aacggcctgc
     9601 actatgtcga gctgcgtttt tcaccaggct acatggcaat ggcacatcag ctgcctgttg
     9661 caggtgttgt tgaagcggtg atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc
     9721 aggcgaagct tatcggcatt atgagccgaa ccttcggcga agccgcctgt cagcaagagc
     9781 tggaggcctt tttagcccac cgtgaccaga ttaccgcact tgatttagcc ggtgatgaac
     9841 ttggtttccc gggaagtctg tttctttctc acttcaaccg cgcgcgtgat gcgggctggc
     9901 atattaccgt ccatgcaggc gaagctgccg ggccggaaag catctggcag gcgattcgtg
     9961 aactgggggc agagcgcatt ggacatggcg taaaagccat tgaagatcgg gcgctgatgg
    10021 attttctcgc cgagcaacaa attggtattg aatcctgtct gacctccaat attcagacca
    10081 gcaccgtggc agagctggct gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg
    10141 ccagcattaa cactgacgat cccggcgtac agggagtgga tatcattcac gaatataccg
    10201 ttgccgcgcc agctgctggg ttatcccgcg agcaaatccg ccaggcacag attaatggtc
    10261 tggaaatggc tttcctcaac gcagaggaaa aacgcgcact gcgagaaaaa gtcgcagcga
    10321 agtaacaaaa atggatggtg caaattgcac catccatttt tcatgcaagg cacaaagtcg
    10381 cgcgatgttt ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc
    10441 ttgccggaac cggattttca ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag
    10501 ccggataatt cccaggcacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc
    10561 gggtaagtac gccatcacgc atatcgtagc cccagtcttc ctgcggcaga cgctcgccat
    10621 ttttcagacg ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat
    10681 gcacgatata ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc
    10741 gctgtggata ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact
    10801 gtgccaaatc taccgtcatg ctgaccggta aaccaaatag cataatggcc tgatcaagaa
    10861 gatgtggtgc taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat
    10921 cgcgcacctg cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca
    10981 cgccttccgc gagcaaacct tttagcgtca agaaatcgct atcccaacga cggttatgga
    11041 atacagacag aacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca
    11101 gtgtcacggt aaagggttta tcaacgacca catgtttacc cgcctcaagc gctgctttgg
    11161 ctaacgggaa atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt
    11221 taaacagatg cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg
    11281 tttcatcact gctggagatt accgccagtt ccagcccggg tgtgcccgca atcaggggcg
    11341 catggaaggt tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca
    11401 tgtcattgcc tctcattgaa gtatgatggc tatttgacac tatcctttac cctcgctcaa
    11461 cagtttaata acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga
    11521 tttttccaac gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta
    11581 ttattgctaa gtaacattta ccccctga
//
LOCUS       DBFIFS010000142        11523 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.142, whole genome shotgun sequence.
ACCESSION   DBFIFS010000142 DBFIFS010000000
VERSION     DBFIFS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.142"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            275..1663
                     /gene="narU"
                     /locus_tag="N7A07_003310"
     CDS             275..1663
                     /gene="narU"
                     /locus_tag="N7A07_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU2477915.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1745..5485
                     /gene="narZ"
                     /locus_tag="N7A07_003311"
     CDS             1745..5485
                     /gene="narZ"
                     /locus_tag="N7A07_003311"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU2477916.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5482..7026
                     /gene="narH"
                     /locus_tag="N7A07_003312"
     CDS             5482..7026
                     /gene="narH"
                     /locus_tag="N7A07_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU2477917.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7026..7721
                     /gene="narW"
                     /locus_tag="N7A07_003313"
     CDS             7026..7721
                     /gene="narW"
                     /locus_tag="N7A07_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU2477918.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7718..8398
                     /gene="narI"
                     /locus_tag="N7A07_003314"
     CDS             7718..8398
                     /gene="narI"
                     /locus_tag="N7A07_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU2477919.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8477..9370
                     /gene="yddE"
                     /locus_tag="N7A07_003315"
     CDS             8477..9370
                     /gene="yddE"
                     /locus_tag="N7A07_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU2477920.1"
                     /translation="MEPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9466..10310)
                     /gene="nhoA"
                     /locus_tag="N7A07_003316"
                     /pseudo
     CDS             complement(9466..10310)
                     /gene="nhoA"
                     /locus_tag="N7A07_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10609..11052
                     /locus_tag="N7A07_003317"
     CDS             10609..11052
                     /locus_tag="N7A07_003317"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU2477921.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11056..11283)
                     /gene="pptA"
                     /locus_tag="N7A07_003318"
     CDS             complement(11056..11283)
                     /gene="pptA"
                     /locus_tag="N7A07_003318"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU2477922.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11382..>11523)
                     /locus_tag="N7A07_003319"
     CDS             complement(11382..>11523)
                     /locus_tag="N7A07_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU2477923.1"
                     /translation="FSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
BASE COUNT         2716 a         2998 c         3030 g         2779 t
ORIGIN      
        1 agttaatata caaccctttg gattcatcat ctgaagggtt gaatcatgac atgcgtacat
       61 agcttaaccc cgtttaatgg ttagcttgtt agttacattt agtaacacat aactggctgc
      121 ttaatcccca ttctgccccc gcggttacgc tttcttatct attctttcaa tcagcacttc
      181 acccatttca tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt
      241 ttttcattac caatgtgtac atgtgaggaa caatatggca ctgcaaaatg agaaaaatag
      301 tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctggaaaa ataaaggaaa
      361 acatattgct cgaagaaacc tctggatatc agtcagttgt ctacttcttg ccttctgtgt
      421 ctggatgcta tttagcgcag ttaccgttaa tctcaataaa atcggtttta atttcactac
      481 cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat tacgtgtccc
      541 ctactccttt atggtgccta tattcggtgg acgtcgatgg acggttttta gtactgcaat
      601 cctgattatt ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg
      661 gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat
      721 gggcaatatc agtttcttct ttccaaaagc caggcaaggt agcgctcttg ggattaatgg
      781 cggattagga aacttaggtg taagtgtaat gcagctggtt gcaccgctgg tcatttttgt
      841 acccgtattt gcctttctcg gcgtcaatgg agtaccgcag gccgacggtt cggtgatgtc
      901 gctggcgaat gccgcatgga tttgggtacc gctactagcg attgccacga tcgccgcctg
      961 gtcagggatg aatgatatcg ccagttcacg cgcgtcaatt gccgaccagc tccctgtctt
     1021 acaacgcctg catctctggc tgctgagcct gctttacctt gccaccttcg gttcgtttat
     1081 cggtttttct gcgggtttcg ccatgctggc aaaaacccag ttcccggatg tgaatattct
     1141 gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cggtcggtgg gtggtgctat
     1201 ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac tttattttta tggcgatttt
     1261 cagcgccctg ctgttcctta ccttaccggg cacaggctcc ggtaacttca tcgcctttta
     1321 cgccgtattt atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat
     1381 gatcgccgtc atctttcgcc agataaccat ttatcggatg aagatgaaag gcggtagtga
     1441 tgagcaagct cagagagaag ccgtcaccga aacggcggcg gctctgggct ttatctcagc
     1501 catcggcgca gtgggcggct tttttattcc gcaggcattt ggcatgtcac tcaatatgac
     1561 cggctctcca gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact
     1621 gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgatg
     1681 tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga gcaggagtca
     1741 tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg atacctttgc
     1801 cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct atcgccagcg
     1861 ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag gctcctgtag
     1921 ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga ccgactaccc
     1981 gcgcactcgc cctgacctgc ccaatcatga acctcgcggc tgcccgcgtg gcgcaagtta
     2041 ctcctggtat ctttacagcg ctaaccgcct gaaatacccg ctcattcgta aacgactgat
     2101 tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg gtactggcgt gggcatcgat
     2161 tatgaacgat ccgcaaaaga gcctgagcta caaacaagtg cgtgggcgcg gcgggtttat
     2221 ccgctccaac tggccggaac taaaccagct gattgccgcc gctaacgtct ggactatcaa
     2281 aacctacggc ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt cgatggtttc
     2341 ttacgccgcc ggaacacgtt atctgtcact attaggtggc acctgtttaa gcttctacga
     2401 ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgagcaaa ccgacgtacc
     2461 ggaatctgcc gactggtata actccagtta catcatcgcg tgggggtcta acgtaccgca
     2521 gacacgtacg ccggacgccc atttctttac cgaagtacgc tacaaaggca ctaaaaccat
     2581 cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc tggcaccaaa
     2641 acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa aagagtttca
     2701 tctcgataat cccagcgact actttatcaa ctactgccgc cgctatagcg acatgccgat
     2761 gctggtaatg ctggaacctc gcgacgatgg tagctacgta cccgggcgca tgatccgcgc
     2821 gtctgacctg gtagatggac taggcgaaag caataatccg cagtggaaaa ccgtggcggt
     2881 taataccgca ggtgaattgg tagtgccgaa tggttcgatt ggtttccgct ggggagaaaa
     2941 aggcaaatgg aatctggaat ccattgccgc cggtaaggaa accgaattgt cgttaaccct
     3001 gctcggtcaa catgacgccg ttgcaggcgt ggctttcccc tactttggcg gcatcgaaaa
     3061 tccgcatttt cgcagcgtca aacacaatcc tgtcctggtg cgtcaattac ccgttaaaaa
     3121 cctgacatta gccggtggca gcacctgtcc agtggtcagc gtttatgatt tggtactggc
     3181 gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt acgctgaaat
     3241 caaaccgtac accccagcct ggggtgagca aattaccggc gtgccgcgcc agtatattga
     3301 aaccatcgct cgtgaatttg ccgatactgc ccataaaacg catgggcgct cgatgattat
     3361 cctcggcgca ggtgttaacc actggtatca catggacatg aactaccggg gaatgatcaa
     3421 tatgctgatc ttctgcggtt gtgtcggaca aagcggtggc ggctgggcac actatgtcgg
     3481 tcaggaaaaa ctgcgcccgc aaaccggctg gttgccgctg gcctttgcgc tcgactggaa
     3541 ccgaccaccg cgccagatga acagcacctc gtttttctac aatcattcca gccaatggcg
     3601 ctatgaaaaa gtctctgcgc aggagttact ttcaccgctt gccgatgcca gtaagtacag
     3661 cggtcatctg attgatttta acgttcgcgc cgaacgcatg ggctggctac cttctgcgcc
     3721 gcagttgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg gattatcccc
     3781 cacagaattt accgcccagg cgctgaaatc gggcgattta cgtatggcct gcgaacaacc
     3841 agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgttctaacc tgcttggctc
     3901 ctccggcaaa ggccacgagt atatgcagaa gtatctgctg gggaccgaaa gcgggattca
     3961 gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa gaagtcgagt ggcaaactgc
     4021 ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgcatgt ccagtacctg
     4081 cctgttctcc gatatcgttc tgcccaccgc cacctggtat gaaaaagacg atatgaacac
     4141 ctcggatatg catccattta ttcatccgct ttctgcggcg gtcgatccgg cctgggaatc
     4201 acgcagcgac tgggaaatct acaaaggtat tgccaaagca ttttcgcaag tgtgcgtggg
     4261 ccatcttggc aaagaaaccg acgtggtatt acaaccactg ctgcatgact ctccggcaga
     4321 gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc tgagcccggg
     4381 taaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacgt atgaacgctt
     4441 tacctcgctc gggccattga tggacaaact tggcaacggc ggtaaaggga tttcgtggaa
     4501 tacgcaggat gaaatcgatt tcctcggtaa actcaattac accaagcgtg atggcccagc
     4561 gcaggggcgt ccgctgattg acaccgccat tgacgcttca gaagtgattc tggcactggc
     4621 accagaaacc aacggtcatg ttgcagttaa agcgtggcag gcgctgggcg agatcaccgg
     4681 acgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct ttcgcgatat
     4741 tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg tctggtctgg aaagcgatca
     4801 cgtctcctat aatgcgggat acaccaacgt tcatgagtta attccgtggc gcacgctgtc
     4861 gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg aaagcctggt
     4921 ggcatatcgc ccgcctatcg acacccgtag cgtcagtgag atgcgccaga tcccgccaaa
     4981 cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg gcattcactc
     5041 aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga ttgtctggat
     5101 cagcgaaacc gatgcccgtg aactaaccat tgtcgataac gactgggtgg aagtgtttaa
     5161 cgccaatggc gcgctgacgg cccgcgcggt ggtcagccaa cgtgtaccgc cgggtatgac
     5221 catgatgtat cacgctcagg aacgcattat gaatattcct ggttcggaag taactggcat
     5281 gcgcggcggt attcataact cggtcacccg catttgcccg aaaccaacgc atatgattgg
     5341 cggttacgcg cagctggcct ggggctttaa ctactacggc accgtcggct cgaaccgcga
     5401 cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg aaggtcgcga
     5461 tcaggtacag gaggcgaaaa aatgaaaatc cgttcgcaag tcggcatggt gcttaatctc
     5521 gataaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg gaccggacgc
     5581 gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat tggttatccg
     5641 aaaaactggg aagatcagga agagtggcaa ggcggctggg tccgcgatgt gaatggcaag
     5701 atacgcccgc gtctgggcag caagatgggt gtaataacca aaatcttcgc caacccggtg
     5761 gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc
     5821 gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cgctgattga cggtaagcgg
     5881 atggacaaag tgatctgggg gccaaactgg gaagaactgc tgggcggcga gtttgaaaaa
     5941 cgtgcccgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac
     6001 accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg
     6061 acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gattgatcag
     6121 gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc
     6181 aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt acccacgaat tgagtccggt
     6241 caaccgaccg tgtgctcaga aacctgcgtg ggtcgcatcc gatatctggg cgtgctgctt
     6301 tacgacgctg accgcatcga ggaagcggcg agcaccgagc gcgaggttga cctctatgaa
     6361 cgccagtgcg aagtgttcct cgatccgcac gatccctcag tgatcgagga agccctgaaa
     6421 caaggtattc cacaaaatgt gattgacgct gcccagcgtt cgccagtcta caaaatggcg
     6481 atggactgga aactggcact accgctgcac cctgaatacc gcaccctgcc gatggtctgg
     6541 tacgttcctc cgctgtcacc gattcagtcc tacgcagatg cgggcggttt gccgaaaagc
     6601 gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct cgccaatatg
     6661 ttgagtgccg gcgataccgg tccggtactg cgggcgctga aacggatgat ggcgatgcgc
     6721 cactatatgc gttcacaaac cgtggaaggc gttactgata ctcgtgccat cgacgaagta
     6781 ggcctgagca tcgcccaggt cgaagaaatg tatcgctatc tcgccattgc caactatgaa
     6841 gatcgttttg ttatcccgac cagccatcgg gaaatggcgg gcgatgcctt cgcagaacgc
     6901 aatggctgcg gttttacctt tggtgacggt tgtcacggct cggacagcaa attcaacctg
     6961 ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa agcggagggc
     7021 gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggac gagatgttgt
     7081 gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga tgcgccgatg cttacggatt
     7141 tcacccgcaa cctgcttaac gcgccgctgc tggataaaca ggccgaatgg tgcgaagtgt
     7201 ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca tgttcatgcc gagtcccgcg
     7261 atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggc ctgcagctgg
     7321 attgtcggga actgcccgat tatctaccgc tgtatctgga gtatttaagc gtactgcctg
     7381 acgatcaggc gaaagaagga ttgctcaacg ttgcgccgat cctcgccctg cttggcggtc
     7441 gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga tgctctgctg caactggcgg
     7501 gaagtacact ttcaagtgac agtgtcacaa aacaagtaaa cagcgaagag cgcgatgaca
     7561 cccgccaggc gcttgatgca gtgtgggaag aggaacaggt gaagtttatt gaagataacg
     7621 ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagt caggatgtcg
     7681 cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg attcagtatc tgaacgtctt
     7741 tttttacgat atctacccgt acatttgtgc gacggtgttt ttcctcggca gctggctgcg
     7801 ctacgactac gggcagtaca cctggcgcgc ctcctcaagt cagatgctcg ataaacgcgg
     7861 gatggtgata tggtcgaatc tgttccatat cggcattttg gggatcttct tcgggcacct
     7921 gttcggcatg ttaacgccac actggatgta cgcgtggttt ttgccagtgg cagcgaaaca
     7981 actaatggcg atggtgctcg gtggtatttg cggtgtgttg acgttgattg gcggcgcagg
     8041 actgctgtgg cgcaggctga caaatcagcg cgtgcgtgcc acttccacta cgccggatat
     8101 catcatcatg agcattttgc tgatccagtg tctgttgggg ctaagtacca taccgttctc
     8161 agcacagtat cctgatggta gcgaaatgat gaagctggtg ggttgggcgc aaagcattgt
     8221 cactttccgt ggtggatcgt cagagatgct taacggtgta gcgttcgtct tccgcctgca
     8281 tctggtgttg ggaatgacca tcttcttgct cttcccattc acccgactgg tgcatgtgtg
     8341 gagcgccccg tttgagtact ttactcgtcg gtatcagatt gtgcgctcgc ggcgataatt
     8401 tcaccctggc cggatagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
     8461 tcacaggaga acgttaatgg aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
     8521 atttcgcggc aattctgccg gtgttgtttt ccccgccgat aatcttagcg aagcgcaaat
     8581 gcagcttatc gcccgcgaat taggtcattc ggaaaccgct tttctgctgc acagcgacga
     8641 cagcgatgtg cgcatccgct actttacgcc aacagttgaa gtgcctattt gcggtcacgc
     8701 gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt ttaggaaatt gcacggtctg
     8761 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcaccacg atggttatcg
     8821 tatttcgctg gaacaaggta cgccaggctt tgagccaccg ctggaaggtg aaacacgtgc
     8881 ggcgattatc aacgcgcttc atctcactga ggacgatatt ctgccaggct tgccaattca
     8941 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
     9001 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
     9061 tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc gatggtcgca tgttctcgcc
     9121 tgcgattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
     9181 gttggtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
     9241 ggggcgcgca ttggggcgtg acggtatgat tgaagtgacg gtgacaattc gtgataacca
     9301 accggaaaaa gtcaccattt ctggcgcggc ggtgattttg tttcatgccg aatgggcaat
     9361 taaactctga aaccgttgcc tgatgcgctt cgcctgtcag gtctacaacc gggctaatgt
     9421 atgccggata aggcgtttac gccgcatccg gcaacctgac ataaattatt ttcccgcctc
     9481 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacggtaa agccatgttt
     9541 cgcatcatcc acgcccagac caaactgttc ttgcatcaca gcatataacg acgccacatc
     9601 cggtagattt cgctgctcca ccgcgtgccc attttcataa tgggaaaatg aaaattggtc
     9661 agtgtcagct tgccgccgtc cggcaaatgg cggcacatca gcaaatgatg gcgaaaatgc
     9721 gactgcggcc agtgcgccga ccagaaatta cccatcacat aatcgctttg ttgctgctcg
     9781 cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg caacacccaa
     9841 tcatcaccct cctgcaacaa ccgatactct ccgtgtggcg tggtctgcac aagatcggga
     9901 actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc aatccatttt
     9961 tcctcttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc tggcggattt
    10021 gataacacta cccgccctaa caagctgcga acgttaaacc ccagctcgcg taacacccgc
    10081 tcaaaaacgc cattctgctc aaaacagtaa ccgccacgac gggctatcac cagtttctct
    10141 tccagcgatt gatcatcaag ctgcatttcc ctcggcagca aaacgtcgag gttttcaaac
    10201 ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg tatcaatatt gaccgcagca
    10261 gctcccgacc agttaatacg ggcaaaatag tgattcagaa tgggcgtcat caggcgtttc
    10321 ccttagtggt tttctcaact tataacctgg aatagacgcc gcaccggcta ttttgtgcga
    10381 tcgcggacat gcatcacgtt cgcaaatcgc caatcccgtt aatttcgtta cttattgcgt
    10441 tacggactac gcttaaaaaa accttcttcg cgaggcgata ccatgagccg attcatcccc
    10501 atagaattac accatgccag ccggctgtta aatcatggcc caactgtcat gatcaccagt
    10561 ttcgatgagc aatcccagcg gcgtaacatt atggctgcag cctgatcaat gccggtggag
    10621 tttgaacctc cacgcgtgac gattgtggta gataaatcga catggaccag ggagttgatt
    10681 gaacgtaacg gtaaatttgg catcgttatc ccgggcgttg cagcaactaa ctggacgtgg
    10741 gcagtgggaa gtgtgtcggg tcgtgaagaa gataaattta attgctatgg cattccggtt
    10801 gtgagaggcc cggtatttgg tttgcctctg gtcgaggaaa aatgtctggc gtggatggag
    10861 tgtcgattgc tacctgcgac ttctgcgcaa gaagaatacg acacgctgtt tggcgaagta
    10921 gtatcagcag cggcagacgc acgggtattt gtcgaaggcc gctggcagtt tgatgatgat
    10981 aagctcaata cgttgcatca tttaggtgct gggacgtttg ttaccagcgg caagcgtgtt
    11041 acggcgggtt aagcattaca tgctgtaacc gggtttcttt atcaaagcct ccatttgggg
    11101 cgcgatttcg gcatcccaga tagcttgcca agattctggt tgaatctgtt gtagagcaat
    11161 gcttatcgaa ctgtctttac tgttcagatg gcgaataata acgtcggtaa tatctgcagc
    11221 aagtgctgct ttttgttgtt cgtccagttc acgcggaaaa catttaatgt ctatgtgcgg
    11281 catgattacg ctccttttga aagatgtcag taaaattaac acacattgtg aatgtcgtgt
    11341 gtccggtaaa gcgtttttac gacaataatg ccagggcaag attacgaaag cccgctcccc
    11401 gcaaggactg acgccagata gtttctgtcc atggctgctt ttcgcatctt acgtcttaac
    11461 cctgccttga ataccttatc attcgtcaaa atattaatag caatgtgccg tatccctgaa
    11521 aat
//
LOCUS       DBFIFS010000143        11496 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.143, whole genome shotgun sequence.
ACCESSION   DBFIFS010000143 DBFIFS010000000
VERSION     DBFIFS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.143"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(282..875)
                     /gene="tehB"
                     /locus_tag="N7A07_003320"
     CDS             complement(282..875)
                     /gene="tehB"
                     /locus_tag="N7A07_003320"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU2477924.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(872..1864)
                     /gene="tehA"
                     /locus_tag="N7A07_003321"
     CDS             complement(872..1864)
                     /gene="tehA"
                     /locus_tag="N7A07_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU2477925.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            1988..2968
                     /gene="ydcK"
                     /locus_tag="N7A07_003322"
     CDS             1988..2968
                     /gene="ydcK"
                     /locus_tag="N7A07_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU2477926.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2963..3499)
                     /gene="rimL"
                     /locus_tag="N7A07_003323"
                     /pseudo
     CDS             complement(2963..3499)
                     /gene="rimL"
                     /locus_tag="N7A07_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(3562..3786)
                     /gene="ydcH"
                     /locus_tag="N7A07_003324"
     CDS             complement(3562..3786)
                     /gene="ydcH"
                     /locus_tag="N7A07_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU2477927.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(3926..5581)
                     /gene="opgD"
                     /locus_tag="N7A07_003325"
                     /gene_synonym="mdoD"
     CDS             complement(3926..5581)
                     /gene="opgD"
                     /locus_tag="N7A07_003325"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU2477928.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5806..7149)
                     /gene="ydcJ"
                     /locus_tag="N7A07_003326"
     CDS             complement(5806..7149)
                     /gene="ydcJ"
                     /locus_tag="N7A07_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2477929.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7366..8289
                     /gene="ydcI"
                     /locus_tag="N7A07_003327"
     CDS             7366..8289
                     /gene="ydcI"
                     /locus_tag="N7A07_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2477930.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8327..9967)
                     /gene="trg"
                     /locus_tag="N7A07_003328"
     CDS             complement(8327..9967)
                     /gene="trg"
                     /locus_tag="N7A07_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU2477931.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGELVSFAPV"
     gene            10366..10515
                     /gene="hokB"
                     /locus_tag="N7A07_003329"
     CDS             10366..10515
                     /gene="hokB"
                     /locus_tag="N7A07_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU2477932.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(10493..10561)
                     /gene="yncP"
                     /locus_tag="N7A07_003330"
     CDS             complement(10493..10561)
                     /gene="yncP"
                     /locus_tag="N7A07_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU2477933.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10587..10760)
                     /locus_tag="N7A07_003331"
     CDS             complement(10587..10760)
                     /locus_tag="N7A07_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477934.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11005..>11496)
                     /gene="cybB"
                     /locus_tag="N7A07_003332"
     CDS             complement(11005..>11496)
                     /gene="cybB"
                     /locus_tag="N7A07_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU2477935.1"
                     /translation="WLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISILVLMV
                     VRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGLVMMYNRGNPWFAF
                     GLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHYFWKDNTLLRMMPR
                     KRS"
BASE COUNT         2982 a         2908 c         2686 g         2920 t
ORIGIN      
        1 tattactttt taagttatag caaaaagcgc cgcttcgtgt tgtggcaaaa aaagtgttgg
       61 tgaaaataaa tttttttagc cccttttcag acgacatagt caatgccgca aaaggggcta
      121 agtcaatcat cagtagttaa atttcagtaa gttaagctgc taaaaaactt cgactactgt
      181 gattaatttc acatcccttc gataaaaaat atgcttaccg aaaagaagga cagtaaacca
      241 tctcagcgtg gggaattttc acgcgctttg ttcttaccgg gtcatttttt acgtgccagc
      301 atcgtggcga aacgcagttt aatacgatta ccgttggcgt cggtgcggtg cagctcgccg
      361 acgtcttcat tgtatttcac catctcccag ccttcgtaat atcgacgtaa ttctccctct
      421 ttgaaggcaa acgggaagcc gacggtacat ggataatcag cggtatccat cgccgccaca
      481 atcaggttgt aaccgccagg tttagtgcaa cgttgcatat tggcaatcag ccctgggatg
      541 gttttagcct caaggaacat cagcaccaca gtcgaaagaa taaaatcgta ctctccatca
      601 aatgtgaggt tattcagatc aacgacccgg gtgtgtaaat tatccagatt ttcaatggat
      661 ttaatgcgct cgacgttggc gatactcatg gcatttttat cccatgcgtc aacatcataa
      721 ccattggctg ccaggtaaag actgttacga ccattgccac agcccagatc cagcgtttta
      781 cccggtttaa ccactttcac cgcttccagt acttcagagt gtgtgcgggt taattcatat
      841 ttatcagtaa aataattttc gtcacgaatg atcattcttt gtcctctgct ttcattaaaa
      901 cggcgcgctc ggttctgact aacaattttc cctgcatcag aagcgccaaa gtacggatga
      961 gcagtattgc aataataaaa ttggtaaaga taaacagcgg caccgccagc gtatggaaaa
     1021 atccattatc gctgccactc cccagatgca aaccggtggt tgccagtgca gatacgccga
     1081 acgagaaact ccagaatgaa gcattaaatg gctgggagag ataccatggc atcagacgta
     1141 gcataaacag cagttgcagc agtccataac cgaaaagcat tttcgccagc gtgtcaccct
     1201 cgccgccgtt gacgctcagc caggcactac aggccaccag cgcaggagcg agctgaatgc
     1261 cgagtgatgt ccgcagtgcc gtgggtaatt ctcccgaact acgcagacgc tgcaagatca
     1321 ccggttcaag gcttagccat gagaaaacgc ctgcgcctaa aaacaccaga ccggcgtcgg
     1381 tgtagcccaa cgcaccacag gccattgcgc tgataaagtt gttggcaact gtcggcagat
     1441 acagtccagg cgtagtagct tcttcagggt gagatccgcg ccataatccc gcagtttgcc
     1501 aggcggcata agccaactga accacgacac caaaactgaa caggcacacc gccagtgggc
     1561 gaaaccacgg aacaaaacca atcgccacca gcatcgtcgt tgcaggaaac aaactcacaa
     1621 aactgctcag cactggatgg cgaacttccg ccagcacgct atgcggaaag cgtatgagtc
     1681 gggtaataaa tgcgctagtc aataatcccc agatgatcat cgccagaatc accagcccat
     1741 cccctaacca gtggctgacc tgccaaacct ggctggcata gcgccaggca aatcccatcc
     1801 ctatcgtccc caacacaata ccaaagtagc ctgccggcaa attgagcact ttatcgctct
     1861 gcatcttctt actcattttg tttaatttat aaagtatatt tgaaatgcat tttatggaat
     1921 tttctcgagc atagccagag ccgcagaatt tgctacggtt atagtaagtc acctgtggga
     1981 aaacgctatg cgcaaatatc gtctaagtga agaacagcga gcctttagtt atcaggaaga
     2041 tggcactaaa aaaaatgtgt tattacggca gattatcgcc attagcgatt ttaacgatgt
     2101 gatagcagga accgctggcg gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa
     2161 ttgctggatt tacgaccaga atgctattgc gttcggcgga acggtgattt ccggtaatac
     2221 gcgcatcacc ggcaccagcg tactgtgggg agaggtttac gcaacggata atgtctggat
     2281 cgataatagt gaaatcaacc agggtgcata tataagtgac agtgtcacca ttcatgactc
     2341 actggtttgc gggcaatgta gaatatttgg tcacgcctta attgatcaac attctatgat
     2401 cgtcgccgca caaggcttaa cgcccgacca tcaactcctg ctccaaattt atgatcgcgc
     2461 aagggttagc gcctcgcgta ttgtccacca ggcacaaatt tatggcgatg ccgtcatccg
     2521 gtatgctttt attgaacatc gcgccgaagt ttttgatttt gccagtattg aaggtaatga
     2581 agagaacaac gtctggctat gcgattgcgc taaagtctac ggtcacgcgc aggtgaaagc
     2641 tggcatagaa gaagatgcca ttccaacgat tcattacagt tcgcaggtgg cggaatatgc
     2701 cattgtggaa ggtaattgtg tgttaaaaca tcatgtcttg attggcggaa atgccgtggt
     2761 acgtggcgga ccgattctgc tcgacgagca cgttgtaata caaggtgaga gccgtattac
     2821 aggtgcggtg ataattgaaa accatgtgga gttaaccgac cacgccgtcg ttgaagcctt
     2881 tgatggcgac acggttcatg tccgtggtcc aaaagtcatt aacggcgagg agcgcattac
     2941 gcgaacgccg ctggcagggt tattatgaat cgataatacg cgcgtataag ttcacatcat
     3001 cataggcatc attcaggaac tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc
     3061 gcaaagcgac ctggttgctt tgcggattgt ccacccgaca tttgatcacg aagcgtctaa
     3121 gttcaccaga ctgagcgtaa tgatgaatca gtgcctgcag cgcctgagaa atgatcccct
     3181 gcccctgatg agattcgtcc agtcagtagc cgatttcagc ggttttattc agtggttcaa
     3241 tacgattaaa cgagataacg ccgataagtt catcttcttt gaaaatcatg aacattttgg
     3301 catagccgcg ttgatgcaac atcacattac cctgcaccgt ttttcgcgtg tcctcttcaa
     3361 tctgaacgaa ctgcggccag tttagcgact gctgtaacca ggttttattt ttacagatta
     3421 actgataaag aggagtgacg tgattttctg caacagcatg taattcaagt gattcgctta
     3481 cttttatcgt ttcagtcatc ttatctccag tcaatccatg cagttgttaa aaaagccgcc
     3541 tggctggcgg cttaggaaag tttacacctc tttgacgctc tcatgctgca ggattttgag
     3601 catctcatct tttaactgta gcttttgttt tttcatgcgg accacttccg cattgtaccc
     3661 tcgatcgtcg gaaccttcct ttctggcaat ttcatgatca agtttattgt gtttatcgaa
     3721 caaggacata aagcgaggat tttcgttttt cagacgggat attaaatctc gatattctgg
     3781 aaacatacgc actccctgtt agtggctgac aaggtgtatc tgaaatacac accatcagat
     3841 tactcattta acttagggaa aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc
     3901 gaagaaggtg ccgaagaaaa aacgattaac tcatcacgcg gtcgtcaaca tactgacgtt
     3961 tatccggcgc tggcgggaaa tactgataca gccatgtttc actgatagcg tcgccctgac
     4021 aacgcagata catccgcata tcgaccggat cagtggagtc cgaagtcgga taccagtcaa
     4081 actgaatacg ataaccatca atgggttcaa tatagagaat ttcgatttgc ttcgcttccc
     4141 cactggaaag cgtaatcacc ggctcaatgc cttttggtgc ggcagctttc agatcaccac
     4201 caacgaaatc gacggcaaaa cgacgcgccc atttttcggg atagtgttca cctggtgccc
     4261 aaccttccgg gaaaccgccc atgccggtac gcgtcgccat aacgcgcgct aatgggcaat
     4321 gaacaggcgg ttgcgcactc cagtacagac gatactggaa tgcaaactca tcacccgcct
     4381 ttacagcttt ttctggctgc cagaagcaga caatgttatc cagcgtttcg cccgttgttg
     4441 ggatttccat caggccgatg gtgcccttac cccacttgtt acgcggttcc acccacagac
     4501 ttgggcgttt gttataccag cccataatgt cctgataatg ggagaaatca cgatccagtt
     4561 gcaataaacc aaaccctttc gggttgttgt cggtgtaagc attgaactgc aatttttgcg
     4621 gattattcag cggacggcaa atccactcgc cgttgccccg ccacatggac agacgatcag
     4681 agtcatgaat ttgcggatga atggtgtcgc acatccgacg ttcattagta ccgcagctga
     4741 acatactggt catcggcgca atgcccagct gtttaatgtc tttgcgcgca tacaggtgat
     4801 tttccacatc cataatcacc tgacttttct cacaatggat agtgaactta taggcaccag
     4861 taatgctggc gctatcgagc aacgcataaa cggtaaaggt agttgccccc ggttttaccg
     4921 tatcaaacca gaaggcggta aagtcgggga actcttcttt actgtcggtg taagtgtcaa
     4981 tcgccaggcc gcgggccgac aaaccgtatt gatatgtgtc atcaacggcg cggaaataac
     5041 tcgctccgag gaatgatact acatcacggc gcgccagttc gggggcttta aacacgcgaa
     5101 aaccggcaaa gccgagatcg ctttgccctt ctaattgttt ggtatcaaca cctgcatcgt
     5161 tgtatttgaa caactccggg cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta
     5221 cagaaaacat acgaacgcgg cgacggaatc ccattcccat atggaagaac tgagcgtcca
     5281 gttgacggtt ctcaacgtta tgccagagcg atttttcggc gtcgtattga atactgttat
     5341 aagcctgcgg cgtcattgtc gccagcgtgt caggtaacgg acgaggcgca ccacgccacg
     5401 ctgtttgcgc taagtcgtgc gccattgact gtagaatgga gaagtcaaaa cgctgggttt
     5461 gcccgtcggc aatatcagaa tctgccgcga atgccgcctg agaaaaaaga gaagcaatgc
     5521 cgctggtacc gcacacggcg gccatagcca ttgaaccttt aataaatcgt ctacgatcca
     5581 tacctgaaag tgagtccttc tggtgttgtg aatggtcccg cgctggtcgt tttcagcggc
     5641 aagaaagtgc ttatttatga ccgctcactc tagacaatat ttattaataa tccaattgtt
     5701 gaactgagag aatatgcata aatcggcaat ttttttatgt cgatgggatg agcctgattc
     5761 gtttgtaaaa tcaggcaaat ttttgcaaag gcggatggtc agaatttaaa gcaaaccaca
     5821 gcgacgttta ctgcgttctt ccgcttcctg ataaagctgg aactcatcca aaaccggaca
     5881 acccaacgcc tgctcaaatg cttcgcgact ggcattaccg tggctgcgtg cctgcgtttc
     5941 attacccaga tttgactgga agatccccgc cgcgctaacg ggcaagaaat cttcataggt
     6001 aatgggttgc gccgcgaccc aaccacgttc aattaagggc tgtggatcgt ctccgggatg
     6061 aatcgcctga cgatgcgcct cacctgaagg cgtcagacgg taccggaacc atgccagccc
     6121 ttgctgacgc attaaaaact cactgtcagg aaaagtgcgg aaggtttcct gtaaatgcat
     6181 ttggtgagtg agattatcct gcccggttcc agcgttacgc agaagatcat catacagttg
     6241 tcgacctttc ggcgttaatg ccacgccacg ctgctcaatt tcaccaaagc gcgcagtatg
     6301 cgtgccctgt ttctgccccg caaacaacac cgtctcttcc agtgctttaa agctggtctg
     6361 gcgtagtaaa atcggtacct cgcggcgcgg cggcccttcg atcagaattt ttggttcaat
     6421 tccgcattca ggcatcatcg actgcacccg gtcaatatcc agcgtacgtg gcgtcagatg
     6481 gttgatatgg catccaggaa aacagaccac atcagcaatc aaccgatgtt cgttgtgcaa
     6541 ggcgcgatag gtttcttcat ctaccgttgc tgactggtgc caacggaacg tttccagggc
     6601 ctcctgcaca aactcctgtg cctgtgtttc gttaaaaccg ccccgctggt catactcctc
     6661 taacagttgt cgacaacgtg gggtgaagat atcgcgctga cgcagaatct ccgccgcttt
     6721 ctggcgcaaa atttcgttct cgataagctc aaggcggagt aaggaggtaa aaacgcggaa
     6781 gggattacgc gccagagaag catcatcaat gggccgaaat gctgtcgaat gtaccggcac
     6841 ccctgcctgc gagagatcgt aatagctcac cgggtacatc cccataatgg caaacatccg
     6901 ccgaagagta gcaagctctt gtgcagtccc aacgcgaatc gccccatgac gttcaacatt
     6961 cagtcgcgcc agctcgtctg catttaccat tttttcgtgc agttgaggat tgttttccag
     7021 cacagccaga ttcacatcag ctaccagttc cagcagcgtg ccatattgcg gaacttcttg
     7081 ctggtacatg gctgacattg cctgcgaaaa ctgttcccga atctcatccg ccgtgatgct
     7141 gttcgccata gtgtctgcct ccagtggtca gtaatctgga gtgtaggtaa ccgcattcac
     7201 tctggcggga agaatttaca aactgtgatc tcgccgcgaa aacatcaata ttatccattt
     7261 tgctgtaaca taattgcttt aattgttaat aatattttgc aatcaagtta tcataatcaa
     7321 acaacttcac ttgtcagcga caccgcttcg tttttaacat cgcttatgga aaaaaatagt
     7381 ctgtttagtc agcgcatccg tttgcgccac cttcatacat tcgtagctgt cgcacaacaa
     7441 ggaactttgg ggcgcgcggc tgaaaccctt aatttgagtc aacctgcgct ctctaagaca
     7501 ttaaatgaac tggagcagct gacgggcgct cgcttgtttg agcgtggtcg tcagggggcg
     7561 caacttacct tacccggcga acaattttta acgcatgcag tcagagtact tgacgccatc
     7621 aacactgccg gacagtcgct tcatcgtaaa gaaggtccta ataatgatgt cgtcagggtt
     7681 ggtgcactac ctactgcggc actggggata ttaccttcgg ttataggtca gtttcatcag
     7741 caacaaaaag agacgacctt gcaagttgcg acaatgagta accctatgat tctggcgggt
     7801 ttgaaaaccg gggaaatcga tatcggcatt ggtcggatgt cagatcctga actgatgacc
     7861 gggcttaatt acgaactgct gtttcttgaa tcgctgaagc tggttgtccg ccctaatcat
     7921 ccgctacttc aggagaacgt aacgctaagc cgggtgctgg aatggccggt cgttgtatca
     7981 ccagaaggca ctgcgccacg ccagcattca gatgcattag tacagagcca gggatgtaaa
     8041 attccttcgg gttgtatcga aacgctgtct gcttcgctat ctcgtcaact tacggttgaa
     8101 tacgactacg tgtggtttgt cccttctggc gcggtaaaag acgacctgcg tcatgccacg
     8161 ctggtggccc tgcctgttcc gggacatggt gcaggcgaac cgattggaat actgacccgc
     8221 gtagatgcga cgttctcttc tggttgccag ttgatgatta acgctattcg aaaatcaatg
     8281 ccgttctgaa aggtgaaggg atctgtcgat ccctccttta acattttcac accggagcga
     8341 aactaactag ttcacccgct ccgcgaggtt ctgccaacac agattgtttg ttcagacgga
     8401 atacgtccac cgcctcagtt aatcgtgccg cctgttcttc aagggacacc gccgccgctg
     8461 aggcctcttc taccagagaa gcattctgtt gcgtcacctt atccatttca gaaatcgcct
     8521 ggctaacctg cgttatgccc ctactttgct catccgaggc ggcggcaatt tcctgcatga
     8581 tatgtgtgac actcgcgacg gcatcaacaa tagtgctcat ggttttcccg gccgttgcca
     8641 cctcatccga tcccaggtca attaacctga ctgattcact gatcaagcct tcaatttctt
     8701 ttgccgcctg ggcgctgcgg cttgcgagtg tccgtacttc gctggcgaca acggcaaatc
     8761 cacgcccttg ctcacccgct cgcgcagctt caacggcagc attcagtgcc agaatattcg
     8821 tctggaaagc aatactgttg atgacggcgg tgatctcaga aattttcttc gaactcgtgg
     8881 agatagcgcc catcgttttt actacaccgg aaaccgtctg cccgccatcg ctggctttaa
     8941 tagaagcctc ttgcgccagt ttgctggcat aatgcgcgtt atccgcattc tgtttcaccg
     9001 tcgcagtgag ttgctccatg ctggcggcag tttgttcgat agccgccgct tgttcttcgg
     9061 tgcgagatga caggtccgca ttgccagctg aaatttcgct ggtgccacga taaatctctt
     9121 ccgcaccctg tcgaacagtc cctacggtca tccccagtga atgctgcatt tgctgtaaat
     9181 gacgacttaa gcgaccgatt tcattacgac ccgccggttc atccttcatc gtcagatcgc
     9241 cactagcgat tttttcaatc cgttgtgcgg catgttgcag tggacgaatg acgatccgac
     9301 gtagcaccat aaatgttatc agcgtcatga ccagggcaag cacaaacgca ccaatcatga
     9361 acatcccgcc caggcgggtg cgctgatggg ccagttccgc cagttggttg gctctggtgc
     9421 tacgtatctt aacggctttg ttcaaaatat cggtataagc attatccagc gttcggatct
     9481 gctcactttc atgattgata atcgcttcaa acatgccatt tttggcatat ttcaacatag
     9541 gttgcatacc cgtgatataa gcctgaaagc gttgatttaa ttcagtgtcg agggcttcat
     9601 cagcaggtgt tttcaccggc cgattctgat aagcacgata accttgctgc gactgtttaa
     9661 tctccgattc ggcttgcgca atatttcgct tcattgcttc catttccgca atacgactcg
     9721 cagccccggc ctgaatcata ttgatccggg ctgaacgcaa aaaatctgaa ctgttcgcta
     9781 acccggtccg aatctcaatc tccgcagtaa catcacgctg atcgcgatcg gcctgccaga
     9841 ggaaatagcc agccagggct gaacttaatg cgaatagaac taagataccg cctagaatgc
     9901 aggcaaataa cggaaccaac ctgatgtgat gcaaaaaacc taatcgctgt gagggagttg
     9961 tattcattac cgttgtctct cgtccaggtt tactcctgat agaaagtcat cggcatttga
    10021 cggtaattac ttacgactaa aagcctcttt gcgtcacatt tttacaacaa ttattgcaga
    10081 gaagattaat gccattggaa tgaagagagt aacgaatggt taaactggct aacccgaatg
    10141 acgaaaaatt ctggaaaaca gcacgcaatt tcgtcaggtg attattttcg tccctaagga
    10201 aaccacatag gatgctcacg ggtgcctgag actttctgtt ttcaggctaa ccgggaatca
    10261 gaaagacgaa ggccccgacc gatatttcta tcaaccgagg ccaacgaatg aaccaagcaa
    10321 ttcagatgtt agcctcttac ccgccatccg gcaaggagaa aggctatgaa gcgcaaccct
    10381 ctggtggtgt gtctgctcat tatctgcatt acgattctga cattcacact cctgacccga
    10441 caaacgctct acgaactgcg gttccgggac agtgataagg aggttgctgc gctcatggcc
    10501 tgcacgtcca ggtaagggca agcgcgggga ttttccccgc gcatctgact agcagattca
    10561 ttttctcatg gcaccccttt tacacttcag ttagtgcatg tttttttcga tgcactaata
    10621 gaaccatcat tacagacaaa tttgccgcct gccgtacagt gtgagatccc ccctttcgaa
    10681 ccagaacagg gtttgcgccc ggcatcggca taaaaggaaa caagcgctcc cataaacaaa
    10741 ataagtgcca gttttttcat ataacctcca tggtttatcg aatcttatgt tcgatttgaa
    10801 cgttatacgc tggtttattt tgataccgtt gatgagatca aagatcgggt gaattgatta
    10861 aaaataagcc agatggacgt atgggcaagg attatggttt tatttgtcat acaaataaat
    10921 ataataggcg cttccgatgt agacccgtat tcttcgcctg taccacgggt cggttttagt
    10981 acaggcgttt tctttaaata tccttcagga acgtttacgc ggcatcatgc gtagaagtgt
    11041 gttgtccttc cagaaatagt ggtgtgccag tgcagccgca gcgtgcaaac cgatgacaaa
    11101 atatcccaga ttcgccagcg tttcatgcca cgactttaag ctatctaccc gttcgaaatt
    11161 ggcctctgaa gcgtaaggca tcgtcaaacc aaacgcaaac cacgggttgc ctcggttata
    11221 catcatcacc aaaccaatca ctggcagcgc aataaacagg agataaatca ccaaatgtcc
    11281 caaatgcgcc agtcccgtca tcatcggttt tggcttaggt ataatcggcg gggttgggta
    11341 tttcagcctt aacaaaagac gaacgaccat cagcacgaga attgatatgc cgcaggaaac
    11401 atgaatcatg ttgatgagtg gccgatcgct acgtgggaag aaaccccgaa actccattgc
    11461 gcaatacgct gcgataacca gcaaaaagac cagcca
//
LOCUS       DBFIFS010000144        11345 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.144, whole genome shotgun sequence.
ACCESSION   DBFIFS010000144 DBFIFS010000000
VERSION     DBFIFS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.144"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..211
                     /locus_tag="N7A07_003333"
     CDS             <1..211
                     /locus_tag="N7A07_003333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477936.1"
                     /translation="RKIRNHASEFTDLIILILIYFEKGIGIYAVFLKILIIGMDFRAR
                     KGFYMDTLLIISSSHHQQFTSLFRV"
     gene            304..2727
                     /gene="pgaA"
                     /locus_tag="N7A07_003334"
     CDS             304..2727
                     /gene="pgaA"
                     /locus_tag="N7A07_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU2477937.1"
                     /translation="MYSSSRKRCPRTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2736..4754
                     /gene="pgaB"
                     /locus_tag="N7A07_003335"
     CDS             2736..4754
                     /gene="pgaB"
                     /locus_tag="N7A07_003335"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU2477938.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4747..6072
                     /gene="pgaC"
                     /locus_tag="N7A07_003336"
     CDS             4747..6072
                     /gene="pgaC"
                     /locus_tag="N7A07_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU2477939.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6074..6487
                     /gene="pgaD"
                     /locus_tag="N7A07_003337"
     CDS             6074..6487
                     /gene="pgaD"
                     /locus_tag="N7A07_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU2477940.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6537..7325)
                     /gene="phoH"
                     /locus_tag="N7A07_003338"
     CDS             complement(6537..7325)
                     /gene="phoH"
                     /locus_tag="N7A07_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU2477941.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7945..9216)
                     /gene="efeB"
                     /locus_tag="N7A07_003339"
     CDS             complement(7945..9216)
                     /gene="efeB"
                     /locus_tag="N7A07_003339"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU2477942.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9222..10349)
                     /gene="efeO"
                     /locus_tag="N7A07_003340"
     CDS             complement(9222..10349)
                     /gene="efeO"
                     /locus_tag="N7A07_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU2477943.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10407..11237)
                     /gene="efeU"
                     /locus_tag="N7A07_003341"
     CDS             complement(10407..11237)
                     /gene="efeU"
                     /locus_tag="N7A07_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU2477944.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         2978 a         2803 c         2658 g         2906 t
ORIGIN      
        1 tcgaaaaatc cgaaatcatg catcggaatt tactgattta attattttaa tcctaattta
       61 ttttgaaaaa ggcatcggga tttatgccgt attcctgaag atcctcatca ttggaatgga
      121 ttttcgggcg agaaaaggat tttatatgga cactctgctc atcatttctt cttctcatca
      181 tcaacaattc acgtctctct tccgcgttta ataacggatt atgaggtgca aaaatatctt
      241 tcttttcagt tacctgtaat tagatacaga gagagatttt ggcaatacat ggagtaatac
      301 aggatgtatt caagtagcag aaaaaggtgc ccgagaacca aatgggcttt gaaacttctt
      361 actgccgcat ttttagcagc gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat
      421 gcattgatta ttgaagctcg caagggtaat actcagccag ctttgtcatg gtttgcacta
      481 aaatcagcac tcagcaataa ccaaattgct gactggttac agattgcctt atgggccggg
      541 caagataaac aggttattac cgtttacaac cgctaccgtc atcagcaatt accagcgcgt
      601 ggttatgcag ctgtcgccgt cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca
      661 ctgtggcaaa aggcgctctc tctggagccg caaaataagg attatcaacg gggacaaatt
      721 ttaaccctgg cagatgctgg tcactatgat actgcgctgg ttaaacttaa gcagcttaac
      781 tctggagcac cggacaaagc caatttactc gcagaagcct atatctataa actggcgggg
      841 cgtcatcagg atgaattacg ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa
      901 caatatccca cagaatacgt gcaggcatta cgtaataatc aacttgctgc cgcgattgac
      961 gatgccaatt taacgccaga tattcgcgct gatattcatg ccgaactggt cagactgtcg
     1021 tttatgccta cgcgcagtga aagtgaacgt tatgccattg ccgatcgcgc cctcgcccaa
     1081 tacgctgcat tagaaattct gtggcacgat aacccagacc gtactgccca gtaccagcgt
     1141 attcaggttg atcatcttgg cgcgttatta actcgcgatc gttataaaga cgttatttct
     1201 cactatcagc gattaaaaaa gacggggcaa attattccgc cctgggggca atattgggtt
     1261 gcatcggctt atctcaaaga tcatcagccg aaaaaagcac agtcaataat gaccgagctc
     1321 ttttatcaca aggagaccat tgccccggat ttatccgatg aagaacttgc ggatctcttt
     1381 tacagccacc tggagagtga aaattatccg ggcgcgctaa ctgtcaccca acataccatt
     1441 aatacttcgc cgcctttcct tcggttaatg ggcacgccta cgagcatccc gaatgatacc
     1501 tggttacagg ggcattcgtt tctctcaacc gtagcaaaat atagtaatga tcttcctcag
     1561 gctgaaatga cagccagaga gcttgcttat aacgcaccag gaaatcaggg actgcgcatt
     1621 gattacgcga gtgtgttaca agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa
     1681 aaagcagaag tgatcgagcc acgtaatatt aatctggagg ttgaacaagc ctggacagca
     1741 ttaacgttac aagaatggca gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa
     1801 ccgcaagatc ccggcgttgt acgattaaaa cgtgcggttg atgtacataa tcttgcagag
     1861 cttcgtatcg ctggctcaac aggaattgat gccgaaggcc cggatagtgg taaacatgat
     1921 gtcgacttaa ccaccatcgt ttattcacca ccgctgaagg ataactggcg cggttttgct
     1981 ggattcggtt atgccgatgg acaatttagc gaaggaaaag ggattgttcg cgactggctt
     2041 gcgggtgttg agtggcggtc acgtaatatc tggctcgagg cagagtacgc tgaacgcgtt
     2101 ttcaatcatg agcataaacc cggcgcgcgc ctgtctggct ggtatgattt taatgataac
     2161 tggcgtattg gttcgcaact ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa
     2221 aatggtgtta caggcaacag tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt
     2281 aagtacggtg tctcctgggc tttcactgat ttttccgaca gtaaccagcg tcatgaagtc
     2341 tcacttgagg gtcaggaacg catctggtct tcaccatatt tgattgtcga tttcctaccc
     2401 agtctgtatt acgaacaaaa tacagaacac gataccccat actacaaccc tataaaaacg
     2461 ttcgatattg ttccggcatt tgaggcaagc catttgttat ggcgaagcta tgaaaatagc
     2521 tgggagcaaa tattcagcgc aggtgttggt gcctcctggc aaaaacatta tggcacggat
     2581 gtcgtcaccc aactcggcta cgggcaacgc attagctgga atgacgtgat tgatgctggc
     2641 gcaacgctac gctgggaaaa acgaccttat gacggtgaca gagaacacaa cttatacgtt
     2701 gaattcgata tgacattcag attttaagga taaatatgtt acgtaatgga aataaatatc
     2761 tcctgatgct ggtgagtata attatgctca ccgcgtgcat tagccagtca agaacatcat
     2821 ttataccgcc acaggatcgc aaatctttac tcgccgagca accgtggccg cataatggtt
     2881 ttgtagcgat ttcatggcat aacgttgaag acgaagctgc cgaccagcgt tttatgtcag
     2941 tgcggacatc agcactgcgt gaacaatttg cctggctgcg cgagaacggt tatcaaccgg
     3001 tcagtattgc tcaaattcgt gaagcacatc gaggaggaaa accgctaccg gaaaaagctg
     3061 tagtgctgac ttttgatgac ggctaccaga gtttttatac ccgcgtcttc ccaattcttc
     3121 aggccttcca gtggcctgct gtatgggccc ccgtcggcag ttgggtcgat acgccagcgg
     3181 ataaacaagt aaaatttggc gatgagttgg tcgatcgaga atattttgcc acgtggcaac
     3241 aagtgcgaga agttgcgcgt tcccggctcg ttgagctcgc ttctcataca tggaattctc
     3301 actacggtat tcaggctaat gccaccggca gcttattgcc tgtatatgta aatcgtgcat
     3361 attttactga ccacgcacgg tatgaaaccg cagcagaata ccgggaaaga attcgtctgg
     3421 atgctgtaaa aatgacggaa tacctgcgta caaaggttga ggtaaatcca cacgttttta
     3481 tttggcctta tggcgaagcg aatggcatag cgatagagga attaaaaaaa ctcggttatg
     3541 acatgttctt cacccttgaa tcaggtttgg caaatgcgtc gcaattggat tccattccgc
     3601 gggtattaat cgccaataat ccctcattaa aagagtttgc ccagcaaatt attaccgtac
     3661 aggaaaaatc accacaacgg ataatgcata tcgatcttga ttacgtttat gacgaaaacc
     3721 tccagcaaat ggatcgcaat attgatgtgc taattcagcg ggtgaaagat atgcaaatat
     3781 caaccgtgta tttgcaggca tttgctgatc ccgatggtga tgggctggtc aaagaggtct
     3841 ggtttccaaa tcgtttgcta ccaatgaaag cagatatttt tagtcgggtt gcctggcaat
     3901 tacgtacccg ctcaggtgta aacatctatg cgtggatgcc ggtattaagc tgggatttag
     3961 atcccacatt aacgcgagta aaatacttac caacagggga gaaaaaagca caaattcatc
     4021 ctgaacaata tcaccgtctc tctcctttcg atgacagagt cagagcacaa gttggcatgt
     4081 tatatgaaga tcttgccgga catgctgctt ttgatggcat attgttccac gatgatgctt
     4141 tgctttcaga ttatgaagat gccagtgcac cggctatcac ggcttatcag caagcaggct
     4201 ttagcgggag tctgagcgaa attcgacaaa acccggagca atttaaacag tgggcccgct
     4261 ttaaaagtcg tgcgttaact gacttcactt tagaacttag tgcgcgcgta aaagccattc
     4321 gcggtccaca tattaaaact gcacgaaata tttttgcact tccggtaata caacctgaaa
     4381 gtgaagcctg gtttgcacag aattatgctg atttcctaaa aagctatgac tggaccgcta
     4441 ttatggctat gccttatctg gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac
     4501 aattgaccaa tcaaattaaa aacatccctc aggctaaaga caaatctatt ttagaattac
     4561 aggcacaaaa ctggcagaaa aatggtcagc atcaggctat ttcttcgcaa caactcgctc
     4621 actggatgag cctattacaa ctgaatggag tgaaaaacta tggttattat cccgacaatt
     4681 ttctgcataa ccaacctgaa atagacctta ttcgtcctga gttttcaaca gcctggtatc
     4741 cgaaaaatga ttaatcgcat cgtatcgttt tttatattat gtctggtgtt atgcataccc
     4801 ctatgcgtag cgtactttca ctctggtgaa ctgatgatga ggttcgtttt cttctggccg
     4861 ttttttatgt ccattatgtg gattgttggc ggcgtctatt tctgggtcta tcgtgaacgc
     4921 cgctggccgt ggggagaaaa cgcaccagct ccccagttga aagataatcc gtctatctcc
     4981 attatcattc cctgttttaa tgaggagaaa aacgttgagg aaaccataca cgccgcttta
     5041 gcacagcgtt atgagaacat tgaagttatt gccgtaaatg acggttcaac agataaaacc
     5101 cgtgccatcc tggatcgcat ggctgcacaa attccccatt tgcgggtcat tcatctggcg
     5161 caaaaccagg ggaaagccat tgcgcttaaa accggagctg ccgcggcgaa aagtgaatat
     5221 ctggtgtgca ttgatggcga tgcgttatta gaccgcgatg cggcggcata tattgtggaa
     5281 ccgatgttgt acaacccgcg tgtgggtgcc gtaaccggta atcctcgtat tcgaacacgt
     5341 tctaccctgg tgggtaaaat tcaggttggc gagtattcct caattattgg tttgatcaag
     5401 cgaacccagc gtatctatgg aaacgtattt accgtttccg gtgttattgc cgcatttcgt
     5461 cgcagcgccc tggcagaagt gggttactgg agtgacgata tgatcaccga agatattgat
     5521 attagctgga agctgcagtt gaatcagtgg acgatttttt acgagccacg ggcactgtgc
     5581 tggatattaa tgcctgaaac gttaaaaggg ctgtggaaac agcgcctgcg ctgggctcag
     5641 ggcggtgcag aagtattcct caaaaatatg acaaggttgt ggcgcaaaga aaactttcga
     5701 atgtggccgc tgttttttga atacagcctg acgacaatat gggccttcac ctgcctggtc
     5761 ggtttcatta tttacgcagt ccaacttgcc ggtgtaccgt taaatattga attgacacat
     5821 atcgctgcga cacatactgc cggaatatta ttgtgtacgt tatgtttact gcaatttatt
     5881 gtcagcctga tgatcgagaa tcgctatgag cataatctga cttcatcgct tttctggatt
     5941 atttggttcc cggttatttt ctggatgctg agcctggcaa cgacattggt atcatttaca
     6001 cgagtcatgt tgatgcctaa aaagcaacgc gcccgttggg taagtcccga tcgcgggatt
     6061 ctgagaggtt aatatgaaca atttaattat tacgacccga caatcaccag tacgtttact
     6121 ggttgattat gttgccacaa ccatcttgtg gacattattt gcgttgttca tattcttatt
     6181 cgccatggat ctgctgacgg gttattactg gcaaagcgag gccagaagcc gacttcagtt
     6241 ctattttttg ctggcagtgg cgaatgccgt cgtgttaatt gtctgggcgc tgtacaataa
     6301 gctgcgtttt caaaaacagc agcatcatgc agcctaccaa tatacgccgc aagaatatgc
     6361 agagagctta gcaatacctg atgagctcta tcagcaacta caaaaaagcc acaggatgag
     6421 cgtacacttc accagccagg ggcaaataaa aatggttgtt tcagaaaaag cgctagtcag
     6481 ggcataaaca cccaaaacaa agcccggttc gcccgggctc tgcaccgata atacacttaa
     6541 ctgtatgcat gcagcgtacg ttggcaaagt gccgaacgta cgcaatcctc ttttccgaac
     6601 cggacgatcc caaccatttc atcttcttcg aaacgttcca gcgcgtcact taatccggag
     6661 cacacgccgc gaggcaaatc gcattgcgtg atatcaccgt tgacgataac cgtcacgttc
     6721 tccccgaggc gggttaaaaa cattttcatt tgcgcggcag tcacattctg cgcctcgtca
     6781 agaatgacga ctgcattttc aaaggtacgt ccacgcatat aggcgaacgg cgcaatttcc
     6841 accttcccta tttccggtcg caggcagtac tgcataaagg aagcccctaa gcgccggacc
     6901 agcacgtcgt agaccgggcg aaaataggga gcaaactttt ctgcgatatc tccaggtaag
     6961 aagccaagat cttcatcggc ttgcagaact ggacgggtga cgataatcct gtcgacatcc
     7021 ttatgtatca gggcctctgc cgcttttgct gcgctgatcc aggtttttcc gcacccggct
     7081 tcgcccgtgg cgaatatcag ctgcttactc tcaatagcct tcagatagtg caattgcgct
     7141 tcatttcgcg cgaggatggg cgaagtatcg cgactgtcgc gggccatacc aatggcttct
     7201 acgccgccca tctgcacaag cgaggtgacc gattcttctt cacgctgctt atggctgcgc
     7261 gaatcccgtc tcagcacacg ttttgcctcg cgacgagctt tgatcactgc tttttgtctt
     7321 cccatggaga gcaccttgag ttgtttgtat tcatcacacg cgccgttggc agcgcgatta
     7381 tgcgcacgaa catcagaggg ttggcttcct tgtaagccat agtttgcttt tggataaaat
     7441 gccgaaaaac ggctacgcgc accgtttacg gcgtcggtaa cacatgaaaa gaaaggatga
     7501 ggttgaaaat gcaaagtgac gagatgacta ccggaggaga aaactccgcg agtggtggcg
     7561 cgttgattat ctaaaacatg tccagtacag gacgttacca tccgcgatct ccatagtgac
     7621 tgactatcac tgccgggaac ttccgctgct acttaataag tacaacagtt ctcgcattta
     7681 ttgcaacaat atatttactt atatttaact ataaaacacc atttcagtga cattagtttc
     7741 tactggaaag atgacagagt gatgacagat atgaaaaaag ctgtgtgctt tcagcaggat
     7801 ttgcatcagg catcagtgca cgatcgccgt atgcggcgtg aacgccttat ccgcctaccg
     7861 aacccgcacc gattgtaggc ataagaagac gcgcaagcat cgcatcaggc atctgcgcaa
     7921 gaacgcctta tccgtctaaa aacattaaac ccgcaataac gcgcttccga gataatcgtt
     7981 cgcgtccttc actcccggca gcgcaaaaaa ataaccgccg ccgataggtt taacgtattc
     8041 ctccagcgct tcgccattga gccttttttg tactgtcagg aagccttttt ccagatcgtg
     8101 ttggtaacag acaaacagta gccccatatc cagttgcccg gagttggtga cgcccagtga
     8161 ataactgtag ccacgacgca gcatcaggct ggactcactc tccgccgtgc ggggattcgc
     8221 cagccggata tggctgtcca gcgcaatccc cttcccttcc gggtcgctgg cgtaatcagg
     8281 cacatcatgc tcatgctgca ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat
     8341 cgtctgctgt tctttcagcg gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg
     8401 tactgcctga tagctgccac cgattgtcca cgcaggctcc tgctgatctg ccgttaccca
     8461 caccactttt tgcatcaact tatcattctg gctatcggga ttagcagtgc cgtctttgaa
     8521 acccagcaaa ttaatcggcg tctctttgcc tttactacgc gccgcgtgat cggaaataaa
     8581 cccttcccgc ttccagcgca cgctgagcaa atccggcgta tgtttgatga tatcgcgcag
     8641 tgcatggatc accgtgtcct gggtgttggc gcaaatttgt agcaacacat caccatgaca
     8701 taacgccgca tccagcgagt cgttgggaaa acgcgtcatc ttctgcaact ttttcggcat
     8761 ctgtggcgca aggccaaagc gctcatcaaa caatgagtga cccaccgata acgtgatggt
     8821 gagattatcg ggcgcaatgt agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg
     8881 atttggcgtt tctggtgccg ctccgccctg agtcagaaaa gcaaaacgct gagtcaacaa
     8941 gcgaaacaac cgttcaagat cggctttatc gctggcaagc acatcaaacg ccaccagcat
     9001 cattgcggcc tgttgtggcg tcaggatccc tgcctgatgc tcaccataaa acggctgttt
     9061 ctcattgcgt gcaaccggtg aaagcgtacc cggcgcactt tgcgtttttt gtgcatgagc
     9121 gaccggacaa cttcccgcca gcgccagcgc accaatgcct ttcagtaaac gtcggcgtga
     9181 cggttcattc acgccgtttt tatcttcata ctgcataatg cttaatccaa tcccagcaca
     9241 ccgcgaagtt gcgccagatc ttccgccagc gcagtaatcg gtcctttcag tgcattccgg
     9301 tcggcatcgg tcaatttgtc gtaggtttca aaaccgtctt tagtacggta tttcgccaga
     9361 atggtatcga cctttttaaa gttggcatcg acttttgcca acagttccgg gttagctttt
     9421 tgcagttgtg gacgcagcag atcgacaatt ttctgcgagc cttcaacgtt agcctggaaa
     9481 tcccacagat cggtgtggct gtagcgatct tcttcaccgc tgattttgct ggctgccact
     9541 tcctcaatca gtccggctgc accgccgacc acttttgaag gtgggaaagc cagttcactg
     9601 atgcgttttt gcaaatcgac cacatcggta taaagctggt cagcgtactg atccatgcct
     9661 ttggtggtgt tgtcgccaaa caatgctttt tccagacggt ggaaaccggt gaattttgga
     9721 tcagcggctt tttgctcgta atcatcttca cgggcgtcaa tgctgccatc cagatcggag
     9781 aacagttcag caatcggttc aatgcgctca tagtgctggc gcgtcggcgc atacagtgct
     9841 ttcgcttttt cgatatcgcc tgctttaatc gcgtcggtaa aggctttggt gtcggtcacc
     9901 agctgtgtgg tttccgccat gacatacgct ttatatgcag taattgcacc accaagactt
     9961 aacagcgcat cactttgcgc cgcatccgcc gttgcctcac ctttgacgat caacttccct
    10021 ttcgggttag tcagcagacc gcaggtcata tcgtattcgc caggctgtaa attcgccgtc
    10081 attttctggc taaagccagg ggcgatattt tcccgctctt ccaccaccat cacgcctttg
    10141 aggatctccc attccagcgc cttctggctg tggttctgaa taatgaactg tgttttcccg
    10201 gcgttaaccg taatggtcat cggttcgcac tgcttatccg tcacggtcac tttgacctgc
    10261 ggcacatcag cggcgttagc cataaaagca gaagaaaaca gcgcagccac gctcaactgc
    10321 aatgcgttac ggcggaagtt aatggtcatg acgagactat ccctttaaag tatgttgtaa
    10381 ctaagcaaga gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc ccctgcgcgt
    10441 ggtggcagag caaatgccac cagcgccggg atgagataaa taaaccagac ggcgacttcg
    10501 ctgacgctcg gcgcttcctg atagccaaaa atcccttcca tcagcgtgcc aaacagcgag
    10561 tgagttgaga gcaccgcact catatcgaag gcgatttcct gaaagtggtt ccacaatccg
    10621 gcttcatgaa atgcgcgaat ggcaccagct gccagccctg cggcgacgaa gagaataaac
    10681 aggctggtcc atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca gtagagcagg
    10741 aagcctagca ccacggcagt agcaagaccg agcattgcac ccagcggcgg ccagatcccg
    10801 acatcttgtt gaaatgccgc cagcaggaaa aagaccgact ccagcccttc ccttgcaacg
    10861 gcaaaaaaga ccatcatcac cagcgcccag ccatgatgat ttccacgctg caatgcgcta
    10921 tcgacagcct gctccagttg tactttgacg ttgcgcgaca ctttgcgcat ccagaaaacc
    10981 atccaggtaa ggatcaccac ggcgatcacc gccacgatac cttcaaacag ttcctgctct
    11041 ttttgcggaa attcgccggt ggtttcgtta atgaagatcc ccaagcccag gcacaacgca
    11101 gcggcaagca acacgccaat ccacatcaca ccaatccatc ggcctcgctg ggtacgctta
    11161 agatagctgg caatcaaact gacaatcagc gcggcttcaa gtccttcgcg caacataatg
    11221 agaaacggaa caaacatgcc acccaccctt aaacgttttt ctgagtcaat ctatgcaaag
    11281 aaaagtaaat cgcagtgata gcgattatca ttacgctaaa gaaaaataca agcggaatat
    11341 ttata
//
LOCUS       DBFIFS010000145        11292 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.145, whole genome shotgun sequence.
ACCESSION   DBFIFS010000145 DBFIFS010000000
VERSION     DBFIFS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.145"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(246..2075)
                     /gene="glmS"
                     /locus_tag="N7A07_003342"
     CDS             complement(246..2075)
                     /gene="glmS"
                     /locus_tag="N7A07_003342"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU2477945.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2237..3607)
                     /gene="glmU"
                     /locus_tag="N7A07_003343"
     CDS             complement(2237..3607)
                     /gene="glmU"
                     /locus_tag="N7A07_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU2477946.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGSGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3959..4378)
                     /gene="atpC"
                     /locus_tag="N7A07_003344"
     CDS             complement(3959..4378)
                     /gene="atpC"
                     /locus_tag="N7A07_003344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU2477947.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4399..5781)
                     /gene="atpD"
                     /locus_tag="N7A07_003345"
     CDS             complement(4399..5781)
                     /gene="atpD"
                     /locus_tag="N7A07_003345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU2477948.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5808..6671)
                     /gene="atpG"
                     /locus_tag="N7A07_003346"
     CDS             complement(5808..6671)
                     /gene="atpG"
                     /locus_tag="N7A07_003346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU2477949.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6722..8263)
                     /gene="atpA"
                     /locus_tag="N7A07_003347"
     CDS             complement(6722..8263)
                     /gene="atpA"
                     /locus_tag="N7A07_003347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU2477950.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8276..8809)
                     /gene="atpH"
                     /locus_tag="N7A07_003348"
     CDS             complement(8276..8809)
                     /gene="atpH"
                     /locus_tag="N7A07_003348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU2477951.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8824..9294)
                     /gene="atpF"
                     /locus_tag="N7A07_003349"
     CDS             complement(8824..9294)
                     /gene="atpF"
                     /locus_tag="N7A07_003349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU2477952.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9356..9595)
                     /gene="atpE"
                     /locus_tag="N7A07_003350"
     CDS             complement(9356..9595)
                     /gene="atpE"
                     /locus_tag="N7A07_003350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU2477953.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9642..10457)
                     /gene="atpB"
                     /locus_tag="N7A07_003351"
     CDS             complement(9642..10457)
                     /gene="atpB"
                     /locus_tag="N7A07_003351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU2477954.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKLQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10466..10846)
                     /gene="atpI"
                     /locus_tag="N7A07_003352"
     CDS             complement(10466..10846)
                     /gene="atpI"
                     /locus_tag="N7A07_003352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU2477955.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2725 a         3054 c         2781 g         2732 t
ORIGIN      
        1 attgatgatt gttttacaag cgattcttga atgaacaaaa cgtgctattc attacaaagt
       61 acatcaatta tggcaattgt ttgtcaatga ttcgaggtat ttaattacta ctacggttaa
      121 aacaagtgtt caacggtgcc ccaaataata gctgaagtta attatatgga atgaggttag
      181 attattaatg ctggtaagta aaagggccgc caaaaggcag cccttcgttc agcaaatgaa
      241 taatattact caaccgttac cgattttgcc aggttacgcg gctggtcaac gtcggtgcct
      301 ttgatcagcg cgacgtgata agccagtaac tgcagcggaa cggtgtagaa gatcggtgca
      361 atcacctctt ccacatgcgg catctcgatg atgtgcatgt tatcgctact tacaaaaccc
      421 gcatcctgct cggcgaaaac atacaattgg ccgccacgcg cgcgaacttc ttcaatgttg
      481 gatttcagtt tttccagcaa ttcgttgttc ggcgcaacga cgataaccgg catatcggcg
      541 tcaatcagcg ccagcggacc gtgtttcagt tcgcctgcag cgtaggcttc agcgtgaata
      601 taagagatct ctttcagctt caatgcgcct tccagcgcga ttgggtactg atcgccacgg
      661 cccaggaaca gcgcgtgatg tttgtcagag aaatcttctg ccagagcttc aatgcgtttg
      721 tcctgagaca gcatctgctc aatacggctc ggcagcgcct gcagaccatg cacaatgtca
      781 tgttcaatgg aggcatccag acctttcagg cgagacagct tcgccaccag catcaacagc
      841 acagttaact gagtggtgaa tgctttagtg gatgccacgc cgatttccgt acccgcgttg
      901 gtcatcagcg ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt acagattgcc
      961 agcgaaccaa ggtaacccag ctctttcgac agacgcaggc ccgccagggt atccgcggtt
     1021 tcgccagact gtgacaaggt gatcatcagg ctgttacgac gcacggcaga tttacgatag
     1081 cggaattcag aggcgatttc gacgtcgcac ggaatacctg ccagcgattc aaaccagtaa
     1141 cgggaaacca taccggagtt ataagaagta ccacaggcga ggatctgaat atgctcaacc
     1201 ttcgacagca gttcgtcggc gttcggtccc agctcgctta aatcaacctg accgtggctg
     1261 atgcgcccgg taagggtgtt tttgatcgcg ttcggctgtt cgtagatctc tttctgcatg
     1321 tagtgacggt aaataccttt atcgcccgcg tcatattgca gattggattc gatatcctga
     1381 cgttttactt ccgcgccagt tttatcgaag atgtttaccg aacggcgagt gatttccgca
     1441 atatcgccct cttcaaggaa gataaagcga cgggtcaccg gcagcagcgc cagctggtca
     1501 gaagcgataa agttttcacc catacccaga ccaatcacca gcggactacc agaacgtgct
     1561 gccagcaggg tatccgggtg acgggagtcc atgatcactg taccgtacgc accacgcagt
     1621 tgcgggatag cacgcagaac agcctcacgc agagtaccgc cctgtttcag ctcccagttc
     1681 accagatggg caatcacttc ggtgtcggtt tcagaaacga aggtatagcc acgcgctttt
     1741 aactcttcgc gcagcggttc atggttttcg atgatgccgt tatgcaccac cacaatgtgt
     1801 tcagaaacat gcggatgcgc attcacttct gaaggttcac cgtgggtcgc ccagcgagtg
     1861 tgagcaatac cggtgccgcc atgcagagga tgttcttccg ctgcctgagc cagcatctgg
     1921 actttaccga ggcgacgcag gcgggtcata tgaccttccg catcaacaac ggccagaccg
     1981 gcagagtcat atccgcggta ttccagacga cgtaaacctt caagaaggat ttctgctaca
     2041 tcacgttgcg cgatcgcgcc aacaattcca cacatagttt ttgattccga tttatatcgt
     2101 tgtcggtcaa cctgtatgcc cgttttttcg ggcgccccga gccttgtaga gagtggggtt
     2161 atttttatag ttactgcttg tgggagggag attatgttat ctcctcatcc catgtgaccg
     2221 gattacccgg ccagaatcac tttttcttta ccggacgacg ccagccttct ttctgagtct
     2281 gcggcacacg gctgatagct aatgcatttt cgccgacatt acgcgtcaca gttgtacccg
     2341 cagcaatggt cgcgcctttg cctactgtta ccggggccac cagctgagtg tcggaaccga
     2401 caaacacatc gtcgccgata atggtcttaa atttattcgc accatcgtag ttgcaggtaa
     2461 tggttcccgc gccgatgtta acgttatcgc caatttccgc atcgccaagg taagtcagat
     2521 gaccagcttt cgagccttta cccagacgtg cttttttcat ctcaacaaag ttaccgacgt
     2581 gtgcaccttc caacaactca gcaccaggac gcaaacgggc aaacgggcca atggtacagg
     2641 ccgccgccag attcgcgtct tccacgacgg tatatgggct aatttcgcaa tcatcgccaa
     2701 tcacgctgtt tttaatcacg caaccggagc cgattttcac gcgatggccg agagtcacgt
     2761 tgccctcgat gataacgtta gtatcaattt caacatcgcg cccgtgagta agcgtaccgc
     2821 gcagatcaaa acgcgccgga tcgcgcagca taacgcctgc taacagcagt ttttcagcct
     2881 gttcggactg gtaaacgcgc tccagacggg agagttgcag gcggttattc acgccttcta
     2941 cttcgcttaa acgttgcgga tgaacggcga cgatttcacg cccttcctga tacgccagcg
     3001 caataatgtc ggtgatgtag tattcgccct gcgcattatt gttggtcagc ttcgccagcc
     3061 agcgtttcat atctgcgccg ttagcgatca gaatgccggt gttgatctcc tgaatctgac
     3121 gctgctcgtc ggtggcatct ttgtgctcaa caatgccggt aactttgccg ttttcacggg
     3181 tgatacgtcc ataaccggtc ggatcatcca gtttcaccgt cagcagacca atgccaccct
     3241 gcggtttagc atcacgcaga cgctggagtg tttcgacaga gatcagcggc acgtcgccgt
     3301 agagcattaa aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc atggcatgac
     3361 ccgtacccag ctgctctgcc tgaagcaccc agttcaggtt gtcgtctttc agcgcctgtt
     3421 ttagcagatc gccaccgtga ccgtacacca ggtgaacgtg cgctgcgcct aattcattcg
     3481 cagcatcaat gacatgctga accatcgctt tcccggcaag ggtatgcagc actttcggaa
     3541 gatcggaata catgcgcgtg cctttacctg cggcaaggat cactacgctc atagcattat
     3601 tcaacatacg cgtcctgact gtaatttgag aacgaattta aaccgcttca ccttgaaaaa
     3661 actacatttt tttcatcgtg aaatggacag aggataaatt gttcaataac ggattatccc
     3721 cgctgacgaa agcgccattt tcgaccatcg tcacgccttt tgtctgcaga aaattaaggc
     3781 agtaccttca tttaagcgtc aggtggatgt ttttgctctt atttcgatca atgaataaac
     3841 gggaaaacag ggggttttat atcagcagga tctatgtgaa cgctattcag gacgggtcac
     3901 acgcgcaaaa aaaagccagc ctgtttccag actggctttt gtgcttttca agccggtgtt
     3961 acatcgcttt tttggtcaac tcgataacgc gcagctgcgc gatcgctttg gccagttccg
     4021 cagacgcctg agcgtaatct acgtcgccgt gagagctgct aatgtgctct tcagccttac
     4081 gtttcgcttc catggctcgc gcttcgtcga gatcctggcc gcgaattgcg gtgtcggcca
     4141 gaacggtcac gttgccaggc tgcacttcaa gaatgccgcc agacagatag ataaactctt
     4201 cgtgaccgtg ctgtttcacg atgcgaatca taccaggctt aatggcggtg agcagcggtg
     4261 cgtggccagg gtagatcccc agttcacctt cgctacccgt tacctggatt ttctcgacca
     4321 gaccagagaa catttgttgc tctgcgctga cgacgtccag gtggtaagtc attgccatat
     4381 caccctccga ttaaggcgtt aaagtttttt ggctttttcc acagcttctt cgatggaacc
     4441 gaccatgtag aacgcctgct ccggcaggtg atcgtattcg ccttccatga tgcctttaaa
     4501 gccacggatg gtgtctttca gggagacgta tttacccgga gaaccggtga atacttctgc
     4561 cacgaagaac ggctgggaca ggaagcgctg gatcttacga gcacgcgcta ccaccagttt
     4621 gtcttcttca gacagttcat ccatacccag gatggcgatg atgtctttca gttcctgata
     4681 acgttgcagg atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag
     4741 cgggtccagc tgacggctgg tggagtccag cgggtcaacg gccgggtaga tacccagaga
     4801 cgcgatctga cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga
     4861 cgggtcagtc aagtcatccg caggtacgta tactgcctgt acggaggtga tagaaccggt
     4921 tttggtggag gtgatacgtt cctgcagaac gcccatctct tccgccaggg tcggctgata
     4981 acctaccgct gaaggcatac ggcccagcag tgcggatact tccgtaccgg ccagggtgta
     5041 acgatagatg ttgtcaacga acagcagaac gtcacgacct tcgtcacgga atttctcagc
     5101 catggtcaga ccggtcagag ctacgcgcag acggtttccc ggcggctcgt tcatctggcc
     5161 atacaccagg gatactttat cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt
     5221 cccctcacga gtacgttcac ctacgcccgc aaacacagag taaccggagt gctcgatcgc
     5281 gatgttacga atgagctcca tcatgtttac ggttttacct acacccgcac caccgaacag
     5341 accaacttta ccgcccttag cgaacggaca catcaggtcg ataactttga taccggtttc
     5401 cagcagttcc tgagagtttg acagctcttc gtaggaaggt gctgcgcggt gaatcgccca
     5461 acgctcttct tcaccgatct cgcctttcat gtcgaccggt tcacccagta cgttcatgat
     5521 acggcccaga gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt cttttacatc
     5581 cagaccgcga cgcagaccgt cggaggaacc cattgcgatg gtacgcacga taccgccgcc
     5641 gagctgctgc tgaacttcca gcaccagacg ctcattacca ttttgcacct caagagcatc
     5701 gtacacgcgc ggtacggcat cctgagggaa ttcgacgtca actacggcgc cgattacctg
     5761 gacaatcttt ccagtagcca tcttaaatcc tctacgaaat aacctgttta aaccgcggcg
     5821 gcccccgaga cgatctcggt gagttcctga gtaatgctgg cctgacgagc tttgttgtat
     5881 accaactgca gctctttaat caggctgccg ccattgtcgg tcgcggcttt catcgccacc
     5941 atacgggcgg cctgctcgct ggccaggttt tcaaccacgc cctgataaac ctgagattcg
     6001 acataacgac gcagcagggt atccagcaac gccttcggat cgggttcgta caggtaatcc
     6061 caggatttat gtttcagatc atcatcatct gatgccggta acggcagcag ctggctgatg
     6121 gtcggaacct gagacatggt gttaataaat ttgttgctga caatgtaaag cttgtccaga
     6181 cggccttcgt cgtaggcctg caacatcact tttaccggac cgatcagttc ggacagggaa
     6241 gggttatccc ccatgccggt gacctgggca acaacattgc cgcccacgga gttgaagaac
     6301 gacacgcctt tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc ggtccaggtc
     6361 ttcatttccg ccagcagttt tttgaacagg ttaatgttca aaccaccgca caaaccacgg
     6421 tcggtcgaca ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag gtaagggtgc
     6481 ttatattcca gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt ttctgcataa
     6541 ggacggctgg ccgccatgcg atcctgcgat ttacgcattt tggaagcggc gaccatctcc
     6601 atcgctttag tgatcttttg cgtgttctgg acgcttgcga tcttactacg tatctctttt
     6661 gcgccggcca tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac
     6721 gttaccagga ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt
     6781 cgtcgttgta gccaccggtc tggttgatct cttgcatcaa cggagcgtga tcacggtcga
     6841 cgtaagccag cagagcggct tcgaagctgc cgattttcga cagttcaaca tccgccaggt
     6901 aaccacgttc tgctgcgaac agaaccagag actgctgcgc aacggacatc ggcgcatact
     6961 gtttctgttt cagcagttcg gtcacttttt gaccgtggtc aagctgctta cgtgttgcat
     7021 cgtcaaggtc ggatgcaaac tgagagaacg ctgccagttc acgatactgt gccagagcgg
     7081 tacggatacc accggacagt tttttcatga tcttggtctg tgctgcacca ccaacacggg
     7141 atacggaaat acccgggtta accgcaggac gaataccggc gttgaacagg ttggtttcca
     7201 ggaagatctg accatcggta atggagatta cgttggtcgg aacgaacgca gaaacgtcac
     7261 ccgcttgagt ttcgataatc ggcagcgcgg tcagtgaacc ggttttccct ttcacttcac
     7321 ctttggtgaa agcttcaacg tattcggcgt taacacgcgc agcacgctcc agcagacgag
     7381 agtggaggta gaatacgtcg cccgggaatg cttcacgtcc tggcggacga cggagcagca
     7441 gcgagatctg acggtaagca acagcctgtt tagacagatc atcgtaaatg atcagcgcat
     7501 cttcaccgcg gtcacggaag tattcgccca ttgcgcaacc ggcatacggt gccaggtatt
     7561 gcagtgcagc ggattcagac gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt
     7621 gctcttccag tttacgtacc acgttagaaa tggtggacgc tttctggccg atagcgacat
     7681 agatacattt gataccggaa tcgcgctggt tgatgatggc atcgatagcc agtgcggttt
     7741 taccggtctg acggtcaccg atgatcaatt cacgctgacc acgaccgatt gggatcatgg
     7801 agtcaacggc tttataaccg gtctgtaccg gctgatctac ggactgacgt tcgataacgc
     7861 ccggagcgat tgcttctaca gcagagaagc cgtcgtgatc cagcggacct ttaccgtcga
     7921 ttggtgcacc cagagtgtta accacacggc ccagcaggcc acggccaacc ggaacttcca
     7981 ggatacggcc agtacactta actttcatgc cttcggcaag gtcagcatac ggacccataa
     8041 caaccgcacc aacagagtcg cgctcgaggt tcagtgcgat agcgtaacgg ttacccggca
     8101 gggagatcat ttcaccctgc atacaatcgg ccaggccgtg aatgcggata acaccgtcac
     8161 ttacagaaac aatagtacct tcgttgtgag cttcactcac aacattgaac tgagcaatgc
     8221 gctgcttgat cagttcgctg atttcggtgg aattcagttg catgctccag tccccttaag
     8281 actgcaagac gtctgcaagg cgctcaagac gaccgcgtac gctgccatca atgaccatat
     8341 cacccgctcg gatgataacg cctgccatta cagacttatc gattttgcaa ttcagcttaa
     8401 ctttgcgtga cagacgtttt tccatcgcag cagaaatttt cgcgagctgt tgttcactca
     8461 gtgcggcagc ggaaatgacg tctacctcag cggtagcctc actcacggca cgaaggtgaa
     8521 taaactgctc caaaacatcc gggagcgcgt taagacgacc attttcagcc attacccgaa
     8581 taaggttctg accgttttcg tccagttgct caccacaaac tgcgataaac gactcggcaa
     8641 gcgtttctgg cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt ttggttacct
     8701 cggcggcaaa cgccagcatg tcctgccagc gttctacact ttggtgttcg acggcaaagt
     8761 caaaagctgc tttggcgtag gggcgagcta ccgtaataaa ttcagacatc agcccctccc
     8821 tccttacagt tcagcgacaa gtttatccac gatgtcgctg ttagcagctt catccacgga
     8881 acgttcgatg atcttctcgg cgccagcaac agccaggata gcaacttgct tacgcagctc
     8941 ttcacgggca cgtttacgct cggcttcaat ttccgcctgc gcctgggcca cgattttagt
     9001 acgttcctgt tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc gtttgttcgc
     9061 ctgctcgatg attacctggg cttccgcttt cgcttttttc agctggtcgg tcgcgctggc
     9121 ctttgcaagg tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc
     9181 tttttgacgt ttttcgatgg ctgccattaa tggcggccat acgtacttca tgcagaacag
     9241 aacgaacagg acaaacgcga tggcctggcc gaggattgtt gcgttaagat tcacagcaca
     9301 atgcctctat ttagttaacg ttctgatatt gctctttaaa taaaagcaac gcttactacg
     9361 cgacagcgaa catcacgtac agacccagac ctacagcgat catcgggata gcatccacca
     9421 gacccataac gataaagaac tgagtacgca gcagaggaat cagatcaggt tgacgcgctg
     9481 cgccttccag gaatttaccc ccgaggatgc cgataccgat cgcagcaccg attgccgcca
     9541 gacccatcat cacagcggca gccatgtaca gcagatccat attcaggttt tccatgacag
     9601 tctccagttt gtttcagtta aaacgtagta gtgttggtaa attaatgttc ttcagacgcc
     9661 atcgacagat agacgatcgt cagaaccatg aagatgaagg cttgcagcgt aatgatcagg
     9721 atgtggaaaa tggcccacgg cacattcagg atccactgtg accaccacgg caacagacca
     9781 gcaatcagaa tgaaaatcag ctcaccggca tacatgttac cgaacagtcg caaaccgagt
     9841 gaaactggtt tggacagcag gcttacccct tcaaggatta agttgacagg aatgaacgcc
     9901 cagtgattga acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg
     9961 atgctgtaga acagaatcag gataaatacg cccagtgcca tagacagcgt tacgttcacg
    10021 tccgcagacg gaaccacacg cagtgcaggc agacccagta catgttcagc aatgtacggc
    10081 agcaggtcga taggcagtaa atccatcagg ttcatcagga atacccagac gaagatcgtc
    10141 agggccagcg gagcaatcag cttgcttttg ccatggtaca tgtctttcac gctaccatta
    10201 acaaagccga tcaccagctc aatcgcggtc tgaagcttac ctggcacacc gctggtcgcc
    10261 tttttggcta cgctacggaa taaaaccagg aacaacagac ccagcaccac cgagaagaac
    10321 atggagtcaa tattgattgt ccagaaggtg gctggggggt tttgtggatc caccagcgag
    10381 aatgtacgca ggtccagctg aaggttattc aggtggtgtc ctatgtaatc ctgcggcgtc
    10441 atattttctg aagccatgat gccttttacc ctttgttgtt aattacagcc ggtgccagta
    10501 tctgaaccac cagcaccaaa acccacgtaa cgatcagcgg caagaatacc gcctttaaaa
    10561 ccgccaacgc caccaccagt aacaccaaca tcgccagaac tttgaaagct tcaccaaatg
    10621 cgaatgtcca ggccacccgg cctttcgctg gtgtatgcgc ctggtgacgc caggcaaata
    10681 tcataaacaa aacgttaggc agaaagactg ccaggccccc gcttattgca gagacgcccc
    10741 agaaggggtc tttgaggctg aacagcaatc cacttgctat caccaccagt aactgaacga
    10801 gcagaagctt ccgagcaacg tttcgactca cgagcgacac agacatcacg tttttcactc
    10861 ctgctccctt cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag
    10921 catcaaaaag cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat aagtagccaa
    10981 aaggtgaata aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt tctactaacc
    11041 ctgttcgatc atgaaaaact gtaaataacg cgtaaacact ggtgataaag cgtgcttcag
    11101 atcacatatt gcgcatgttc gcgcacagca tatttattta cttggcaaat gatgcctttg
    11161 caagtttatg atatttcagt ctaaaaacag atactgtttt aataaatgac atttacacaa
    11221 caaaaaccac ccattgacat ttttaataat gttttaacag ccaatgatgg ctcttagcgc
    11281 cgatttttag ca
//
LOCUS       DBFIFS010000146        11258 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.146, whole genome shotgun sequence.
ACCESSION   DBFIFS010000146 DBFIFS010000000
VERSION     DBFIFS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.146"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            418..2007
                     /gene="purH"
                     /locus_tag="N7A07_003353"
     CDS             418..2007
                     /gene="purH"
                     /locus_tag="N7A07_003353"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU2477956.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            2019..3308
                     /gene="purD"
                     /locus_tag="N7A07_003354"
     CDS             2019..3308
                     /gene="purD"
                     /locus_tag="N7A07_003354"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU2477957.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3305..4630)
                     /gene="zraR"
                     /locus_tag="N7A07_003355"
     CDS             complement(3305..4630)
                     /gene="zraR"
                     /locus_tag="N7A07_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU2477958.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4627..6003)
                     /gene="zraS"
                     /locus_tag="N7A07_003356"
     CDS             complement(4627..6003)
                     /gene="zraS"
                     /locus_tag="N7A07_003356"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU2477959.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            6241..6666
                     /gene="zraP"
                     /locus_tag="N7A07_003357"
     CDS             6241..6666
                     /gene="zraP"
                     /locus_tag="N7A07_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU2477960.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(6668..7363)
                     /gene="yjaH"
                     /locus_tag="N7A07_003358"
     CDS             complement(6668..7363)
                     /gene="yjaH"
                     /locus_tag="N7A07_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU2477961.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7376..7648)
                     /gene="hupA"
                     /locus_tag="N7A07_003359"
     CDS             complement(7376..7648)
                     /gene="hupA"
                     /locus_tag="N7A07_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU2477962.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            complement(7835..8425)
                     /gene="yjaG"
                     /locus_tag="N7A07_003360"
     CDS             complement(7835..8425)
                     /gene="yjaG"
                     /locus_tag="N7A07_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU2477963.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            complement(8468..9139)
                     /gene="nfi"
                     /locus_tag="N7A07_003361"
     CDS             complement(8468..9139)
                     /gene="nfi"
                     /locus_tag="N7A07_003361"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU2477964.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            complement(9149..10213)
                     /gene="hemE"
                     /locus_tag="N7A07_003362"
     CDS             complement(9149..10213)
                     /gene="hemE"
                     /locus_tag="N7A07_003362"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU2477965.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            complement(10253..11026)
                     /gene="nudC"
                     /locus_tag="N7A07_003363"
     CDS             complement(10253..11026)
                     /gene="nudC"
                     /locus_tag="N7A07_003363"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU2477966.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            11121..>11258
                     /locus_tag="N7A07_003364"
     CDS             11121..>11258
                     /locus_tag="N7A07_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HGU2477967.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SY"
BASE COUNT         2611 a         3163 c         2937 g         2547 t
ORIGIN      
        1 ctttcagagt caaccctgaa tttcagaatt tttctcttca accgaaccgg ctgtttgtgt
       61 gaagtgattc acatccgccg tgtcgatgga ggcgcattat agggagttct ccgcaggccg
      121 caatagaaaa attgcagaaa aatgactgac tgctgcattc cccagcaaaa ccccgcttta
      181 taccttttta cgcacagagt tatccacaat catcagcgtt atttctctat ttctcccacg
      241 ctaatcgggg gcaaaatcgt gatcgccatc gaattaaaaa aattcgcgag cgttgcgcaa
      301 acgttttcgt tacaatgcgg gcgaaaaata aggatgcccc gttaggggcg ttagctgagt
      361 ttttcgcgaa aaattcagct aacgctctct gtaatagtca aatccagggg atttaccatg
      421 caacaacgtc gtccagtccg ccgcgctctg ctcagtgttt ctgacaaagc cggtatcgtc
      481 gaattcgccc aggcactttc cgcacgcggt gtggagctgc tgtctacagg gggcactgcc
      541 cgtctgttag cagaaaaagg tctgccggta accgaagttt ccgattacac cggtttcccg
      601 gagatgatgg atggacgcgt gaagaccctg catccgaaag tacatggtgg cattctgggc
      661 cgtcgcggcc aggacgatgc cattatggaa gaacatcaga tccagcctat cgatatggtg
      721 gttgttaacc tgtatccgtt cgcccagacc gtggcccgtg aaggttgctc gctggaagat
      781 gcggttgaga acatcgatat cggcggccca acgatggtgc gctccgccgc caagaaccat
      841 aaagatgtcg caatcgtggt gaagagcagc gactacgacg ccattattaa agagatggat
      901 gacaacgaag gatcgctgac gcttgcaacc cgtttcgacc tcgccatcaa agccttcgaa
      961 cacactgccg cctacgacag catgattgcc aactacttcg gcagcatggt tccggcttat
     1021 cacggtgaaa gcaaagaagc cgccggtcgc ttcccacgca cgctgaacct gaacttcatt
     1081 aagaagcagg atatgcgtta cggcgagaac agccaccagc aggctgcctt ctatatagaa
     1141 gagaatgtga aagaagcctc cgttgctacc gcaacccagg ttcagggtaa agccctctct
     1201 tataacaaca tcgccgatac cgatgcggcg ctggagtgcg tgaaagagtt cgccgagccg
     1261 gcatgtgtga ttgtgaagca cgccaaccct tgcggcgtgg ctatcggcaa ttcaattctt
     1321 gatgcttacg atcgcgcgta caaaaccgac ccgacctccg cattcggcgg catcattgcc
     1381 tttaaccgcg agctggatgc ggaaaccgca caggccatca tttctcgtca gtttgttgaa
     1441 gtgattattg cgccgtccgc cagcgaagaa gccctgaaaa tcaccgccgc caaacagaat
     1501 gtacgcgttc tgacctgcgg tcagtggggc gagcgtgttc cgggtcttga tttcaaacgc
     1561 gtgaacggcg gtctgctggt tcaggatcgc gatctgggca tggtcggtgc ggaagaactg
     1621 cgcgtcgtca ccaaacgtca gccgaccgaa caggaactgc gtgatgcgct gttctgctgg
     1681 aaagtggcga agttcgtgaa atccaatgct atcgtctatg ccaaaaacaa tatgaccatc
     1741 ggtattggcg cgggccagat gagccgcgtg tactccgcga aaatcgccgg tattaaagct
     1801 gccgatgaag gcctggaagt gaaaggttcc tcgatggctt ctgacgcgtt cttcccgttc
     1861 cgcgacggta ttgatgccgc cgccgctgcg ggcgtgacct gcgtaatcca gcctggcggt
     1921 tccatccgtg atgacgaagt gattgccgcc gccgacgagc acggtattgc gatgctcttc
     1981 accgatatgc gccacttccg ccattaatgg agcgacagat gaaagtatta gtgattggta
     2041 acggcgggcg cgagcacgcg ctggcctgga aagcggccca gtcgccgctg gttgagactg
     2101 tttttgttgc tccgggtaac gcaggcacag cgctggaacc cacgctgcaa aacgtcgcta
     2161 tcggcgtgac cgatatcccg gcgctgctgg atttcgcgca aaacgaaaag gtagatctaa
     2221 ccatcgtcgg cccggaagcg ccgctggtga aaggcgtggt cgataccttc cgcgccgccg
     2281 ggatgaaaat cttcggccca accgcaggcg ccgcacagct ggaaggctcg aaagcgttta
     2341 ctaaagattt cctggcccgc cataacattc ctacggcgga atatcagaac ttcaccgagg
     2401 tagaacctgc gctggcgtat ctgcgtgaga aaggcgcgcc aatcgtcatt aaagcggacg
     2461 gtctggctgc cgggaaaggc gttatcgtgg cgatgacgct ggaagaagcg gaagcggctg
     2521 ttcacgatat gctggcgggc aacgcgtttg gcgacgcggg tcatcgcatc gttatcgaag
     2581 agttcctcga cggcgaagaa gcgagcttta tcgtgatggt ggacggcgag catgtgctgc
     2641 cgatggccac cagccaggat cacaaacgcg taggcgataa agataccggg ccgaacacgg
     2701 gcggaatggg cgcttactct cctgcgccgg tagtgacaga tgacgttcat cagcgcacga
     2761 tggaacgtat catctggcca accgtgaaag gcatggcgtc ggaaggcaac acctacaccg
     2821 gttttctcta cgcgggcctg atgatcgaca aacagggcaa tccgaaggtt atcgaattta
     2881 actgccgctt tggcgatcca gaaacccagc cgattatgct gcgcatgaag tccgatcttg
     2941 ttgaactctg cctggcggcc tgtgaaggca aactagacga gaaaacgtca gagtgggatg
     3001 aacgtgcttc tctcggcgtg gtgatggctg cgggtggata tccgggcgat taccgcaccg
     3061 gtgacgtgat ccacggcctg ccgctggaag aagtggaaga cggcaaagtg ttccacgcgg
     3121 gcacaaaact ggcggatgac gagcaggtgg taaccagcgg cgggcgcgta ctgtgcgtca
     3181 ccgcgctggg tcataccgta gcagaagcac agaaacgcgc ctatgcctta atgaccgata
     3241 tccactggga cgactgcttc tgccggaaag atatcggctg gcgcgctatc gaacgcgagc
     3301 agaactaacg cgacaatttt gccaacagcg ttttgcgcgt gatccctaac tgacgggcgg
     3361 cttcggtttt gttgccgccc gttttctcca gtgccgccag aatcacttct ttttcgactt
     3421 ccaccagcgg ctgaatatcc aggctttgtg ccagcgggat cggcgtactg gcaatcgcca
     3481 gcggcagctc gcgttcggaa atatattccc cagtcagcag caccaccgcc cgttccaccg
     3541 cattttccag ttcacgaata tttcccggcc agtcgtaatg aatcaacaga tccatcgcct
     3601 ggggcgtaaa gccttttacc gccttgcgat tacgttcagc aaagcgctgc agaaaatggc
     3661 cagccagcag aggaatatct tcccgccgtt gccgcagcga tggtacttca atcgccacca
     3721 cgttcaggcg atagtagaga tcctggcgaa aacgcccggc attcacctct gcggcaagat
     3781 cgcgatgggt cgcggcaatc agccggacat caaccgaaat agtctggttg ctaccgacac
     3841 gctgaacttc gcgctcctga atcgcacgca gcagacgcac cagcatcatc ggcgagatat
     3901 cgccaatttc atcgagaaac aacgtgccgc cgtccgcctc aacaaagcgc ccttcccggc
     3961 gtttatcggc tccagtaaac gccccttttt cgtgaccgaa caattcagat tccagcaagg
     4021 attcgttgag tgccgcacag ttgagcgtta ccagcggttt ttcgctacgt gcgctactgg
     4081 cgtgaatcgc cctggcgacc agctctttac cggtgccgga atcgccgtgg atcagcaccg
     4141 tggcttccga tggcgcgacg agggcgattt cactgagcag gtgttgcatc gccgggcttt
     4201 taccgaccat accgaactgg ctggcagtca ccgcaggcgt ttcagcatca ataatgtgcg
     4261 tatgcgcgag cgccttttcc agcgtcgcct gcaggttatc gaaatccagc ggcttgatga
     4321 gataatccag cgccccggtt ttcaacgcct ctaccgccgt ctcgacgcta gagtatgcgg
     4381 tcataatcag taccggaatc gccggattta acgctttgat ctcttttagc gtggcaatgc
     4441 cgtccatctc cgccattcgc acatcgcaaa gcacaagatc aaaaacccgc tcccgcacct
     4501 gctccagcgc ctgtcgcccg ctgttcgcca gcgcgacgtt atagccccag ccgcgcagca
     4561 aagcctgcaa aatagtgcag tggctaatgt catcatccac caccagaata tcgatattat
     4621 cgtgcgtcat ccttgtgggt ccttacgcgt aatattgacc ggaagccaga gggtgaacct
     4681 tgcgcctttt ccctccaggc ttgcgacctg aattgtacca ccgtgttgtt caacaatatt
     4741 atgcacgacc gccagcccca gtccggtgcc ttcggctttg gtggtgaagt acggggtgaa
     4801 gatggcttca agctgatctg ccgcaattcc cttaccgctg tcggtaacgc tgattttcac
     4861 gcccgcgccg ctttcgctgg ccgtcacgct aatcacgcca tgctgaccaa tcgcctgaat
     4921 agcattgaga taaagattca acaggacctg agtcagcctg tccggatcgg cctgaatttc
     4981 cggcaacgtg tcgttggcgg taaagcgtaa ctggatctcc cggcagtttg catcctggct
     5041 taccagctgt aatgagtggt taatcagcgt gttgagatcc accgcctgca aagccagatg
     5101 cgttggctta accagttcca gcagctcgct taccacgcgg tttaaacggt cagcttcttt
     5161 cgccatcacc tgcgccagtt gatgcgcttc tccccctgct ggcgcgcgct cggcaaagta
     5221 ttttgccagc cctttaatcg aggaaagcgg gttacgaatt tcgtgggcaa cgcctgccgc
     5281 aagatgcccc agcgccacca gcttctcttt gcgcttcatt tcatcttgta gaagctggcg
     5341 cgagcgcaga tagcggcgat accagaagaa tgacaatacg cttgccagca agaccgtcgc
     5401 cagggcgaag aggataatca gggtgtttct ctgctcacga tcttcggcac tcacaatatt
     5461 actggcgtcg aaagcgatga aaattgcttg tggtgtcgcg gcatactgct gcatatggcg
     5521 catccggtgc attccagcag caaacatcgg ttgaaactgg cgataaattt ccagaacagg
     5581 ctcgccgtct gcgctatcga tccgccgcca cacttcttca tctcccggat gtaactgctg
     5641 catttcctgc ggggaataaa gctgttttcc caccatgccg gagttgctat gcatcacgat
     5701 tgttccttgt tcatccgtga ccgcaaacca acgtactcca ggctgcccgg ccatttcttc
     5761 cagtaaggcc tgctgctgcg catgatgcat gcgcatcccc atgccgacgc gcgagccgga
     5821 ttcgagagca cgaataagta cactgccttt ttccagcagc gtttgtctgg cggcggcagt
     5881 ctcgcggcca taatcgcgaa tcaccgtcac cgcaaacaac cctaccagcc caacaatgac
     5941 cacggggagg atcgcgctta accatttagc taaggagtct ttagaacgtt gcataaaacg
     6001 catcttcttc tttgcctgct catccctttc tgacagcaga aatcatgcca tcttttatca
     6061 gcgcttaccc tgcgctgtaa cacaaaggct taagtttcaa tgagtaaaaa tgactcgcta
     6121 cccgcagcag gcgagtcatt tttactcgtt tatcatgcca gattacccgt catatcagcg
     6181 tttcatcgtt ggcacggaag atgcaatacc cgaagtaaga caaccactgg aggattaacc
     6241 atgaaacgga acacgaaaat tgccctggta atgatggcgc tttcagcaat ggcgatggga
     6301 tcgacatctg catttgctca cggcggacac ggtatgtggc agcaaaatgc cgcgcctttg
     6361 accagcgaac aacagacagc gtggcagaaa atccataatg acttttacgc tcaaagcagc
     6421 gcactgcaac agcaactggt gacgaagcgt tatgaataca atgccctgtt agccgcgaac
     6481 ccaccggata gcagcaaaat taacgcggtc gccaaagaga tggagaattt gcgtcagtcg
     6541 ttagatgagt tacgggtgaa acgggatatt gcgatggctg aagcgggtat tccgcgcgga
     6601 gccggaatgg gcatgggcta cggcggctgc ggtggtggcg gtcatatggg tatgggccac
     6661 tggtaaatca gaacgttttc tcgttgggtt gccagcgaca gaaatcagag tttgccacta
     6721 acagtaattg cgaaccttcg ggcgcttcca gccatgccac gctgacatca acagaagagc
     6781 ggctctggcg gcgctcgata tgcgctattg cctgcgaatc taaatcaggt ttaatggttt
     6841 gcccttcgca ggtggtcacg gtctgttcca tcgcatgcca gcgcccctgg cgcagcacga
     6901 ctctcccctg acgtaaggca tcgctaatct ggcggatctg atcagcccga taacggtata
     6961 aatcgatttg gtcgttagaa agctgctgct tttccccatc ggattcacgt tgcataaagc
     7021 tcagatcacc gcgatcgtca aaacgcgcgc gaatattcac cgggggtttg ctgtaaacat
     7081 tgatattgat gagcgtgagg ttatcgccct gccagcgata ctctcgcgtc gtggtatcgc
     7141 cactgcgcca cgggctaaac acggcaagaa aatgcacatt atcgccgctg tcttttcgcc
     7201 agatgcgtac cgcgccctgg tcttcagcaa atccactggc ggtaaaggga ggaagtgaag
     7261 agttgtgact acaagcagtg agaaacagag cgccagccag catcaatgat cgacgccaga
     7321 aagacaaaag gggtgaaacc accccttcgt taaaactgtt cactgccacg caatcttact
     7381 taactgcgtc tttcagtgcc ttgccagaaa caaatgccgg tacgttagct gcggcaattt
     7441 tgatttcttt accggtctgc gggttgcggc cagtacgctc agcgcggtgg ttcactttga
     7501 aggtaccgaa accaaccagt tgtacagcat cgccttcttt cagagactca gtaattgcag
     7561 ccagagtgga ctccagagca gctttagcct gggttttgga cagttctgct ttctctgcaa
     7621 ttacatcaat cagttgagtc ttgttcataa gttatcctta caatgtgttt atcgcttgct
     7681 aagcatcgag tgcgacggaa atgccagaat agcactctcc tgcatacacg caccgatagc
     7741 cacttttttt cgccccccaa atgtagacca gacggggggc gaaagggaag actccaggta
     7801 cgacaaatca ggcgttaaat cacgttttct ggtcttattg ctgaaaaatt ataccaatat
     7861 tgctctcatc cgcctcacgc aggtctgccc taaggccttt tatcagctcg atatcgcgtt
     7921 cttcgcactc agctaaaagt cggaatattt cccactgaat gtcccattct tgctctacag
     7981 ctgggttttc tttgagctct tcatcgctca tttcgcgacc agcctgagtc atttccagca
     8041 tcgcaacggt cgtgatggag gtcttactca cttccaccgc gtgttcgagc gtttcaccac
     8101 tcaaacgcga atggaccagt tcacttaacg ccacgcaggc atcgattgcc ggataaacgc
     8161 cgtacagatc gaaatcgtcg gctgaaggaa tcgcttcttc aaatttctcc agttggctgt
     8221 cgaaatttac ttttgcatct ttaacggtca gcgtttccca gatgagatcg agaatacgac
     8281 ggtaaatttg cccatcacca aaaccggttt gctggcagaa catggcgtaa ttggggtaca
     8341 tgcgttcgca taagcaagcc atgaaagtga catgctgcca gctttccagg cgttccagac
     8401 gcagatgaat tgggttttgt aacatgttga gttctcaaat acggaaatta tccgcagttt
     8461 acctgaatta gggctgattt gctgtatagc gcacgaacgc cggacgttcc gaggccaccg
     8521 cgtccgccca gcgcgttggc tccggcagac gatagccttt catgcagcgt tgtacccacg
     8581 ccagcgcgct gtccacgctg acccgatgac cagtagcgat aaatagcggg ttacagcgcg
     8641 ctttgctgcg ccagacccag gccagctgct cgcctttatc catcagtggg gccagcgcgc
     8701 ccggttcgct ggagagcggt tcgaatttac cgcatagtcg ttttttcgct acgccaatgg
     8761 tcggcacatc caccagcaaa ccaaaatggc tggcgacgcc aagacggcga ggatgcgaga
     8821 tcccatgacc atcgacaaac actaaatccg gcttttgtga cagcatctcc cacgctgcca
     8881 gcagcgcagg atattcgcgg aaggaaagaa aacctggaat gtaaggcatg gtggtagcga
     8941 tgcgggccac tttatactcc accagctcaa gcgaggggta tttcagcagc accatcgccg
     9001 ctcgcgtcac ttctccgccc tgctcaaacc cgacatcggc tccggcgatc agatccggcg
     9061 gatccttatc gagtcgatcc tcgcggatca cagaagaagc cagttcgatt tgttgagcgc
     9121 gtaatgacgc gagatccata atcgctcctt agcggtgata ctgttcagac agtcgatgca
     9181 ctgcctccac gaacacgcca gcatgttctg gcggcacatc ctgatgaatg ccgtgaccaa
     9241 ggttaaagac atgaccttcg ccgtgaccga aacctgcaag tatagtcgct acttcttctt
     9301 caatgcgggc aggcggcgcg tacagcatcg acggatccat attaccctgc aacgcgactt
     9361 tattgcccac acggcggcgc gcatcggcga tgtccgttgt ccagtcgagg cccagcgcat
     9421 cgcaaccggt ttctgccatg gcttccagcc actgtccgcc gcctttggta aacagcgtga
     9481 ccggtacgcg gcgaccgtcg ttttcacgca gtaaaccatc aacaatttta tgcatgtaat
     9541 aaagcgagaa ctgttgataa tcgcgcccgg taagcacgcc gccccaggtg tcgaaaatca
     9601 tcacagcctg agcaccggct ttaatctgcg cattcagata caaagcgacg cttttcgcca
     9661 gtttatcgag tagagcgtgc agcgcctgcg gatcggcata catcattttt ttgatcacgg
     9721 tgaaggcttt gctgctgccg ccttccacca tgtaggtcgc cagcgtccac gggctgccgg
     9781 aaaaaccaat cagcggcact tcgcctttca gttcgcgacg aatggtacgc accgcgttca
     9841 tcacgtaccc cagttcatct tccgggtccg gaattggcag tttatcgacg tcagctttgc
     9901 aggtgactgg cgaggtaaaa cgcggacctt ctccggcttc aaaatagagc cctaacccca
     9961 tcgcgtccgg gacggtgagg atatcggaaa agaggatcgc cgcatccagc gggtagcgac
    10021 gcagcggttg caaggtcact tcacacgcca gctcggcgtt tttgcacagc gacataaaat
    10081 cgcccgcctg ggcgcgcgtg gctttatatt ccggtagata gcgacccgcc tggcgcatca
    10141 tccatacagg agtgacatca acgggctggc gcagcagcgc ccgcagataa cgatcgtttt
    10201 taagttcggt cattttggct gttccttagt gcgtcaggcg gtcagtgtat catcactcat
    10261 actctgcccg acacatcgcc accgtatctt ctatcagacg gcgcgctacg gtgccgggcg
    10321 gcgggagtaa cggcaaatcg tcatagcgat accagttcgc ctcgagcaat tctttcgggt
    10381 cgatcacgat gtcgccgctg tcatattccg ccataaacgc ggtcattaaa gactgaggaa
    10441 acggccacgg ctgggaagtc acataacgca agtgtttaac tttaattccg ctctcttcca
    10501 tcacttcccg cgcgaccgcc tgctcgaggg tttcgcccac ttcgacgaat ccggcaagta
    10561 ccgtatggac accgttacga tggcgggtat gctgggcgag gaggatcgaa tcatcgcgac
    10621 ggatggcaac aataatgcag ggggcgattt gcgggtagta acgctcacgg caatggctgc
    10681 acagcatcgc ccattcggtt ttgcttggat acatttcatg cccgcagtaa ccacagtatt
    10741 tatgcgatcg gtaaaactcc gccagttgta cgccacgtcc ggccagttga aacagcccaa
    10801 catcgagatc aatgacctga cgtaccgacc ccatatcgta acgccgctgc tgttgtatta
    10861 accaaacagg ttccccctgc cattcgccga tttgtagtgc gcgctgaccc acaagatcga
    10921 aatttgccgc ttcgccatat ggcaattctc ccttcggcaa ccataatttt tgttcatggc
    10981 tgacgaccca ccagccgtga tctaattttt caattatacg atccatagct cttgcactac
    11041 ctttgcatca ctggcatgtt taacatggtt tttacatttc tcactgagca gtttttgaat
    11101 acaaacttgc ggagtcaatc atgcttaacc agctcgataa cctgacggag cgcgtcagag
    11161 gaagtaacaa actggttgat cgctggctac atgtacgtaa gcatctgctc gtggcttact
    11221 acaatctggt tggcattaag cctggcaaag aatcgtac
//
LOCUS       DBFIFS010000147        11174 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.147, whole genome shotgun sequence.
ACCESSION   DBFIFS010000147 DBFIFS010000000
VERSION     DBFIFS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.147"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            405..2333
                     /gene="thrS"
                     /locus_tag="N7A07_003365"
     CDS             405..2333
                     /gene="thrS"
                     /locus_tag="N7A07_003365"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU2477968.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2337..2879
                     /gene="infC"
                     /locus_tag="N7A07_003366"
     CDS             2337..2879
                     /gene="infC"
                     /locus_tag="N7A07_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU2477969.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            2976..3173
                     /gene="rpmI"
                     /locus_tag="N7A07_003367"
     CDS             2976..3173
                     /gene="rpmI"
                     /locus_tag="N7A07_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU2477970.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3226..3582
                     /gene="rplT"
                     /locus_tag="N7A07_003368"
     CDS             3226..3582
                     /gene="rplT"
                     /locus_tag="N7A07_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU2477971.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3699..3823
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3705..3749
                     /gene="pheM"
                     /locus_tag="N7A07_003369"
     CDS             3705..3749
                     /gene="pheM"
                     /locus_tag="N7A07_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU2477972.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4032..5015
                     /gene="pheS"
                     /locus_tag="N7A07_003370"
     CDS             4032..5015
                     /gene="pheS"
                     /locus_tag="N7A07_003370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU2477973.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGCTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5030..7417
                     /gene="pheT"
                     /locus_tag="N7A07_003371"
     CDS             5030..7417
                     /gene="pheT"
                     /locus_tag="N7A07_003371"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU2477974.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7422..7721
                     /gene="ihfA"
                     /locus_tag="N7A07_003372"
     CDS             7422..7721
                     /gene="ihfA"
                     /locus_tag="N7A07_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU2477975.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7822..8802
                     /gene="btuC"
                     /locus_tag="N7A07_003373"
     CDS             7822..8802
                     /gene="btuC"
                     /locus_tag="N7A07_003373"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU2477976.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8865..9416
                     /gene="btuE"
                     /locus_tag="N7A07_003374"
     CDS             8865..9416
                     /gene="btuE"
                     /locus_tag="N7A07_003374"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU2477977.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9416..10165
                     /gene="btuD"
                     /locus_tag="N7A07_003375"
     CDS             9416..10165
                     /gene="btuD"
                     /locus_tag="N7A07_003375"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU2477978.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10243..10707
                     /gene="nlpC"
                     /locus_tag="N7A07_003376"
     CDS             10243..10707
                     /gene="nlpC"
                     /locus_tag="N7A07_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU2477979.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10954..>11174
                     /gene="ydiV"
                     /locus_tag="N7A07_003377"
     CDS             10954..>11174
                     /gene="ydiV"
                     /locus_tag="N7A07_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU2477980.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQH"
BASE COUNT         2807 a         2635 c         3011 g         2721 t
ORIGIN      
        1 tgaccacttc gtcattaatc ttatgatcgt ttaaatagta ccgccaaatc acgcaacgaa
       61 ctttgctgga ttcagtttat gctgctgtaa attcgctcgc agtaaacctt tcagacgctc
      121 cgtgatgtta tcagttgttc tttaagcgtt ttgctggtgt actcactaca aacgaattgc
      181 gaatcgaatc aatgtgaaac ggaaaggtac aatctccctt tttttagtgt taacatcgct
      241 caaccgggtg gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt gcgaaccaat
      301 ttagcatttg ttggctagat ggtttcgcaa tgaactgtta ataaacaaat ttttctttgt
      361 atgtgatctt tcgtgtgggt caccactgca aataaggata taaaatgcct gttataactc
      421 ttcctgatgg cagtcaacgc cattacgacc acgctgtaag ccccatggat gttgcgctgg
      481 acattggtcc aggtctggcg aaagcctgta tcgcagggcg cgttaatggc gaactggttg
      541 atgcttgcga tctgattgaa aacgacgcac aactgtcgat cattaccgcc aaagacgaag
      601 aaggtctgga gatcattcgt cactcctgtg cgcacctgtt agggcacgcg attaaacaac
      661 tttggccgca taccaaaatg gcaatcggcc cggttattga caacggtttt tattacgacg
      721 ttgatcttga ccgcacgtta acccaggaag atgtcgaagc actcgagaag cggatgcatg
      781 agcttgctga gaaaaactac gacgtcatta agaagaaagt cagctggcac gaagcgcgtg
      841 aaactttcgc caaccgtggg gagagctaca aagtctccat tcttgacgaa aacatcgccc
      901 atgatgacaa gccaggtctg tacttccatg aagaatatgt cgatatgtgc cgcggtccgc
      961 acgtaccgaa catgcgtttc tgccatcatt tcaaactaat gaaaacggca ggggcttact
     1021 ggcgtggcga cagcaacaac aaaatgttgc aacgtattta cggtacggcg tgggcagaca
     1081 aaaaagcgct taacgcttac ctgcagcgcc tggaagaagc cgcgaaacgc gaccaccgta
     1141 aaatcggtaa acagctcgac ctgtaccata tgcaggaaga agcgccgggt atggtattct
     1201 ggcacaacga cggctggacc atcttccgtg aactggaagt gtttgttcgt tctaaactga
     1261 aagagtacca gtatcaggaa gttaaaggtc cgttcatgat ggaccgtgtc ctgtgggaaa
     1321 aaaccggtca ctgggacaac tacaaagatg caatgttcac cacgtcttct gagaaccgtg
     1381 aatactgcat taagccgatg aactgcccgg gtcacgtaca aattttcaac caggggctga
     1441 agtcttatcg cgatctgccg ctgcgtatgg ccgagtttgg tagctgccac cgtaacgagc
     1501 cgtcaggttc tctgcatggc ctgatgcgcg tgcgtggatt cacccaggat gacgcgcata
     1561 tcttctgtac tgaagaacaa attcgcgatg aagttaacgg atgtatccgt ttagtctatg
     1621 atatgtacag cacttttggc ttcgagaaga tcgtcgtcaa actctccact cgtcctgaaa
     1681 agcgtattgg cagcgacgaa atgtgggatc gtgctgaggc ggacctggcg gttgcgctgg
     1741 aagaaaacaa catcccgttt gaatatcaac tgggtgaagg cgctttctac ggtccgaaaa
     1801 ttgaatttac cctgtatgac tgcctcgatc gtgcatggca gtgcggtaca gtacagctgg
     1861 acttctcctt gccgtctcgt ctgagcgcct cctatgtggg cgaagacaac gagcgtaagg
     1921 taccggtaat gattcaccgc gcaattctgg ggtcgatgga acgtttcatc ggtatcctga
     1981 ccgaagaatt cgctggtttc ttcccgacct ggcttgcgcc agttcaggtt gttatcatga
     2041 atattaccga ttcacagtct gaatacgtta acgaattgac gcaaaaacta tcaaatgcgg
     2101 gcattcgtgt taaagcagac ttgagaaatg agaagattgg ctttaaaatc cgcgagcaca
     2161 ctttgcgtcg cgtcccgtat atgctggtct gtggtgataa agaggtggaa tcaggcaaaa
     2221 ttgccgttcg cacccgccgt ggtaaagacc tgggaagcat ggacgtaaat gaagtgatcg
     2281 agaagctgca acaagagatt cgcagccgca gtcttaaaca attggaggaa taaggtatta
     2341 aaggcggaaa acgagttcaa acggcgcgcc ctaaccgtat caatggcgaa attcgcgccc
     2401 aggaagttcg cttaacaggt ctggaaggcg agcagcttgg tattgtgagt ctgagagaag
     2461 ctctggagaa agcagaagaa gccggagtag acttagtcga gatcagccct aacgccgagc
     2521 cgccggtttg tcgtataatg gattacggca aattcctcta tgaaaagagc aagtcttcta
     2581 aggaacagaa gaaaaagcaa aaagttatcc aggttaagga aattaaattc cgtcctggta
     2641 cagatgaagg cgactatcag gtaaaactcc gcagcctgat tcgctttctc gaagagggtg
     2701 ataaagccaa aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta
     2761 tggaagtgct taatcgcgtg aaagacgatt tgcaagaact ggcagtggtc gaatccttcc
     2821 caacgaagat cgaaggccgc cagatgatca tggtgctcgc tcctaagaag aaacagtaag
     2881 gccttcaagt agcactgtct gtggagcctt cgggcttcac aggctttgtt cgcctttgtt
     2941 tcgtttatta acaatgcgaa gtggaagtta ttaaaatgcc aaaaattaag accgtacgcg
     3001 gtgctgctaa gcgcttcaaa aaaaccggta aaggtggttt taagcacaag cacgctaacc
     3061 tgcgtcacat tctgaccaaa aaagcgacca aacgtaaacg tcacctgcgt ccgaaagcca
     3121 tggtttccaa aggcgatctg ggcctggtaa tcgcgtgcct gccgtacgca taagccgtta
     3181 acgtttttaa ctttttaatt agaatataga tacaggagag cacatatggc tcgcgtaaaa
     3241 cgtggtgtta ttgcacgcgc acgtcacaag aaaattttga aacaagctaa aggctactac
     3301 ggtgcgcgtt ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc tggtcagtat
     3361 gcttaccgtg accgtcgtca acgtaagcgt cagttccgtc aactgtggat tgcgcgtatc
     3421 aacgcagcag cacgtcagaa cggtatttct tacagcaaat tcatcaatgg cctgaaaaaa
     3481 gcctctgttg aaatcgaccg taagatcctg gctgatatcg cagtattcga caaagtagcg
     3541 ttcaccgctc tggttgaaaa agcgaaagca gctctggcat aagccagttg aaagagggag
     3601 ctagtctccc tcttttcgtt tcaacgccat caaaacattg acttttatcg ccgtagcctt
     3661 ttcaataaag gtcttttgaa gagtaccaaa aggtaacgca agcaatgaat gctgctattt
     3721 tccgcttctt tttttacttt agcacctgaa tccaggaggc tagcgcgtga gaagagaaac
     3781 ggaaaacagc gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg ttttgaaaaa
     3841 taaaaagcca gtctatcagg cgtaattgtc gcagccagtc tggatacggc cagtgcggag
     3901 aaaccggagc gtacttaagt acgtgaggat ttcgagcact gcccgggacc aaaatggcaa
     3961 gtaaaatagc ctgatgggat aggctctaag tccaacgaac cagtgtcacc actgacacaa
     4021 tgaggaaaac catgtcacat ctcgcagaac tggttgccag tgcgaaggcg gccattagcc
     4081 aggcgtcaga tgttgccgcg ttagacaatg tgcgcgtcga atatttgggt aaaaaagggc
     4141 acttaaccct tcagatgacg accctgcgtg agctgccgcc agaagagcgt ccggcagccg
     4201 gtgcggttat caacgaagcg aaagagcagg ttcagcaggc gctgaatgcg cgtaaagcgg
     4261 aactggaaag cgctgcactg aatgcgcgtc tggcggcgga aacgattgat gtctctctgc
     4321 caggtcgtcg cattgaaaac ggcggtctgc atccggttac ccgtaccatc gaccgtatcg
     4381 aaagtttctt cggtgagctt ggctgtaccg tggcaaccgg gccggaaatc gaagacgatt
     4441 atcataactt cgatgctctg aacattcctg gtcaccaccc ggcgcgcgct gaccacgaca
     4501 ctttctggtt tgacgctacc cgcctgctgc gtacccagac ctctggcgta cagatccgca
     4561 ccatgaaagc ccagcagcca ccgattcgta tcatcgcgcc tggccgtgtt tatcgtaacg
     4621 actacgacca gactcacacg ccgatgttcc atcagatgga aggtctgatt gttgatacca
     4681 acatcagctt taccaacctg aaaggcacgc tgcacgactt cctgcgtaac ttctttgagg
     4741 aagatttgca gattcgcttc cgtccttcct acttcccgtt taccgaacct tctgcagaag
     4801 tggatgtcat gggtaaaaac ggtaaatggc tggaagtact gggctgcggg atggtgcatc
     4861 cgaacgtgct gcgtaacgtt ggcatcgacc cggaagttta ctctggtttc gccttcggga
     4921 tggggatgga gcgtctgact atgttgcgtt acggcgtcac cgacctgcgt tcattcttcg
     4981 aaaacgatct gcgtttcctc aaacagttta aataaggcag gaatagatta tgaaattcag
     5041 tgaactgtgg ttacgcgaat gggtgaaccc ggcgattgat agcgatgcgc tggcgaatca
     5101 aatcactatg gcgggcctgg aagttgacgg tgtagaaccg gttgccggta gcttccacgg
     5161 cgtggtcgtt ggtgaagtgg ttgagtgtgc gcagcatccg aacgctgaca aactgcgtgt
     5221 gacaaaagtg aatgtcggcg gcgatcgcct gctggacatc gtctgcggtg cgccaaactg
     5281 ccgtcagggc ctgcgtgtgg cggtagcgac cattggtgct gttctgccgg gtgatttcaa
     5341 aattaaagcg gcgaaactgc gcggcgaacc gtctgaaggg atgctgtgct ccttttctga
     5401 gctgggaatt tctgacgacc ataacggcat tatcgaactg cctgcagatg cgccgattgg
     5461 cactgacatc cgcgaatacc tgaaactcga tgacaacacc atcgaaatca gcgtaacgcc
     5521 aaaccgtgcc gactgtttag gtatcattgg tgttgcgcgt gacgttgccg tgctgaatca
     5581 gctgccgctg gttgaaccgg aaatcgttcc ggttggtgcg accatcgacg acacgctgcc
     5641 gattacagtc gaagcgccgg aagcctgccc gcgttatctt ggccgtgtgg taaaaggcat
     5701 taacgttaaa gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt gcgggatccg
     5761 ttctatcgat gcagttgttg acgtcaccaa ctatgtgctg ctcgaattgg gccagccgat
     5821 gcacgctttc gataaagatc gcattgaagg cggcattgtg gtgcggatgg cgaaagaggg
     5881 cgaaacgctg gtgctgctcg acggtactga agcgaagctg aatgctgaca ctctggtcat
     5941 cgccgaccac aacaaggcgc tggcgatggg cggcatcttc ggtggcgaac actctggcgt
     6001 gaatgacgaa acacaaaacg tgctgctgga atgcgctttc tttagcccgc tgtctatcac
     6061 cggtcgtgct cgtcgtcatg gcctgcatac tgatgcgtct caccgttatg agcgtggcgt
     6121 tgatccggca ctgcagtaca aagcgatgga acgtgcgacc cgtctgctga ttgacatctg
     6181 cggtggtgag gctggtccgg taattgatat caccaacgaa gcaacgctgc cgaagcgtgc
     6241 aaccatcact ttacgtcgta gcaaactgga tcgcctgatc ggccatcata ttgcggatga
     6301 gcaggtaact gacattctgc gtcgtctcgg ctgcgaagtg accgaaggca aagacgagtg
     6361 gcaggcagtt gcgccgagct ggcgtttcga catggagatt gaagaagatc tggtcgaaga
     6421 agtcgcgcgt gtttacggct acaacaacat cccggatgag ccggtacagg caagcctgat
     6481 tatgggtact caccgtgaag ctgacctgtc gctcaagcgc gtgaaaacgc tgctcaacga
     6541 taaaggctat caggaagtga tcacctatag cttcgttgat ccgaaagtgc agcagatgat
     6601 ccatccaggc gttgaagcct tactgctgcc aagcccgatc tctgttgaaa tgtcagcaat
     6661 gcgtctttct ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga accgtcagca
     6721 gaaccgtgtg cgcattttcg aaagcggtct gcgtttcgta ccagatactc aggcaccgtt
     6781 gggcattcgt caggatctga tgttagccgg tgtgatttgc ggtaaccgtt acgaagagca
     6841 ctggaacctg gcaaaagaga ccgttgattt ctatgatttg aaaggcgatc ttgaatccgt
     6901 tctcgacctg accggtaaac tgaatgaggt tgagttccgt gcagaagcga atccggcact
     6961 gcatccgggg caatccgcag cgatttatct gaaaggtgaa cgtattggtt ttgttggggt
     7021 tgttcatcct gaactggaac gtaaactgga tcttaacggt cgcactctgg tgttcgaact
     7081 ggagtggaac aagctcgcag accgcgtggt gcctcaggcg cgcgagattt ctcgcttccc
     7141 ggcgaaccgt cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag cggatatttt
     7201 atccgaatgt aagaaagttg gcgtaaatca ggtagttggc gtaaacttat ttgacgtgta
     7261 ccgcggtaag ggtgttgcgg aggggtataa gagcctcgcc ataagcctga tcctgcaaga
     7321 taccagccgt acactcgaag aagaggagat tgccgctacc gtcgccaaat gtgtagaggc
     7381 attaaaagag cgattccagg catcattgag ggattgaacc tatggcgctt acaaaagctg
     7441 aaatgtcaga atatctgttt gataagcttg ggcttagcaa gcgggatgcc aaagaactgg
     7501 ttgaactgtt tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag gtgaaactct
     7561 ctggttttgg taacttcgat ctgcgtgata agaatcaacg cccgggacgt aacccgaaaa
     7621 cgggcgagga tattcccatt acagcacggc gcgtggtgac cttcagaccc gggcagaagt
     7681 taaaaagccg ggtcgaaaac gcttcgccca aagacgagta atctgatcta actaaaaagg
     7741 ccgctctgcg gccttttttc ttttcactgt cgaagagtca ccgtaaaatc aacgccatga
     7801 cacttcagca gaacggatac catgctgaca cttgcccgcc aacaacagcg acaaaatatt
     7861 cgctggttat tatgcctgtc agttttgatg ctgctggcgc ttctcttaag cctttgcgcc
     7921 ggtgaacaat ggatctcgcc aggtgactgg ttttctcctc gtggcgaact gttcgtctgg
     7981 caaattcgcc tgccacgtac gctggctgta ttgctggttg gtgcggcgct ggctatatcc
     8041 ggcgctgtaa tgcaggcgtt gtttgaaaat cctctggcag aacctggact acttggcgtc
     8101 tctaacggcg caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg gcaactcccc
     8161 aactgggcgc tagggctgtg tgcgattgct ggggcgctta tcatcacttt aatactctta
     8221 cgtttcgccc gtcgtcatct ttcgaccagt cggttattgc tggctggcgt tgcattaggg
     8281 attatctgta gcgcactaat gacgtgggct atctactttt ccacctctgt tgatttacgt
     8341 cagctgatgt actggatgat gggcggtttt ggcggcgtag actggcggca aagctggctg
     8401 atgctggcat tgatccccat gttgttgtgg atctgttgtc agtccaggcc gatgaatatg
     8461 ttagcacttg gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt ctggcgcaat
     8521 gtgctggtgg cagcgaccgg ctggatggtt ggcgtcagtg tagcgctggc gggtgctatc
     8581 ggctttattg gtctggtgat cccacatatt cttcggttgt gtggtttaac cgatcatcgc
     8641 gcattacttc ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc cgatattgta
     8701 gcgcgcctgg cattagctgc cgcagagctg cctattggcg tggtcaccgc aacgttgggt
     8761 gcgccggtgt ttatctggtt attgttaaaa gcaggacgtt agccgcaaaa agacggtcta
     8821 tgattaaaag ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc
     8881 tgacgaccgt agtgaaagat atcgacggtg aagtgaccac gctggagaag tacgccggta
     8941 atgtgctgtt gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt
     9001 tggagaatat tcagaaagcc tgggccgatc gaggttttgt ggtgctggga ttcccgtgca
     9061 accagtttct ggaacaagaa ccgggcagcg atgaagagat taaaacttac tgtaccacca
     9121 catggggggt gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgccatc
     9181 cgctgtatca aaaattgatt tccgcagcgc cgaccgcagt cgcgccggaa gagagcggat
     9241 tctatgcccg tatggtcagc aaaggtcgtg caccgctgta ccctgatgat attttatgga
     9301 attttgagaa atttttagtt ggcagggacg gattggtcat ccagcgtttt tccccggata
     9361 tgacgccgga agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc
     9421 tattgtgatg cagttacagg atgttgcgga atctacccgc ctggggccgc tttctggcga
     9481 ggttcgggct ggggagatcc tgcacctggt ggggccgaat ggcgcgggta agagtacctt
     9541 actggcgcga atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc
     9601 actggaagca tggtccgcaa caaaactcgc gctgcatcgt gcctatcttt cacaacagca
     9661 gacgccgccg tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac
     9721 gcgtaccgaa ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggtcg
     9781 tagcaccaat caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgct
     9841 gcaaatcaca ccacaagcca atcccgcagg ccaattgctg cttcttgatg agccgatgaa
     9901 cagtcttgat gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgcgc tgtgtcagca
     9961 aggactggcg attgtgatga gcagtcacga tctcaaccac acattgcgtc atgcgcatcg
    10021 ggcgtggttg ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag atgtgctcac
    10081 gccgccaaat ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca
    10141 cagaatgctg atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac
    10201 gctaaattaa tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt
    10261 ttgatcacag cactgtttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc
    10321 agactttctg attcgattac cgttattgcc ggtttgaacg accagctaca aagctggcat
    10381 ggcacgccgt atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg
    10441 gttgtgacga tgcgcgatcg tttcgatttg cagctgcccc gagaaaccaa agaacaagcc
    10501 tctatcggca cgcaaattga taaagacgag ttgctgcctg gtgacctggt ctttttcaaa
    10561 acgggttccg gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc
    10621 cacgcctcta ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa
    10681 aatttctggc aggcgagacg aatctagtat gtcgaaaatt ggacgtcgat gatacatcgc
    10741 cgtccattta ctccagaata cagtagtatt ttgcagacaa aaattccgta aatggattat
    10801 tcgtttataa tcaggataat ctaaaaaaag gacccctgaa aaagtggtta acatggaaca
    10861 aatgaaagtt actccttctg taattgttat gtcttatggc ctcatccctt ttagccggat
    10921 actgaaaaac atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat
    10981 cattctgatt gctatttcct gccgataaga gataaccagc aagtccttgt cggcgttgag
    11041 ttaattaccc atttctccag tgaagatggc acggtgcgta tcccgacgag tcgggttata
    11101 gcgcaactta ccgaggagca acattggcaa ttattttcag agcagttgga attactaaaa
    11161 tcatgccaac attt
//
LOCUS       DBFIFS010000148        11117 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.148, whole genome shotgun sequence.
ACCESSION   DBFIFS010000148 DBFIFS010000000
VERSION     DBFIFS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.148"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(263..328)
                     /locus_tag="N7A07_003378"
                     /pseudo
     CDS             complement(263..328)
                     /locus_tag="N7A07_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            550..2235
                     /gene="btsS"
                     /locus_tag="N7A07_003379"
     CDS             550..2235
                     /gene="btsS"
                     /locus_tag="N7A07_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU2477981.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            2232..2951
                     /gene="btsR"
                     /locus_tag="N7A07_003380"
                     /gene_synonym="yehT"
     CDS             2232..2951
                     /gene="btsR"
                     /locus_tag="N7A07_003380"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU2477982.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2998..3468
                     /gene="yehS"
                     /locus_tag="N7A07_003381"
     CDS             2998..3468
                     /gene="yehS"
                     /locus_tag="N7A07_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU2477983.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3510..3971)
                     /gene="yehR"
                     /locus_tag="N7A07_003382"
     CDS             complement(3510..3971)
                     /gene="yehR"
                     /locus_tag="N7A07_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU2477984.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(4096..6099)
                     /gene="yehQ"
                     /locus_tag="N7A07_003383"
     CDS             complement(4096..6099)
                     /gene="yehQ"
                     /locus_tag="N7A07_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU2477985.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(6096..7232)
                     /gene="yehP"
                     /locus_tag="N7A07_003384"
     CDS             complement(6096..7232)
                     /gene="yehP"
                     /locus_tag="N7A07_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU2477986.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTPPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(7225..9504)
                     /gene="yehM"
                     /locus_tag="N7A07_003385"
                     /pseudo
     CDS             complement(7225..9504)
                     /gene="yehM"
                     /locus_tag="N7A07_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(9515..10603)
                     /gene="yehL"
                     /locus_tag="N7A07_003386"
     CDS             complement(9515..10603)
                     /gene="yehL"
                     /locus_tag="N7A07_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU2477987.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2640 a         2844 c         2937 g         2696 t
ORIGIN      
        1 attttttctt tagcgataca gatctcaaca cgcataacac cctcctgtaa ctgtagttat
       61 gtacaggtgt tatttttatc tgtatggata accagtgtca attcctgata ttgtttatgg
      121 ggcatcaatg gggcatgtat gggacacttt ttatcggcga aattcgtcga agttcatcga
      181 catggtaaac gaatcatcta tccaaccctt gaaaaacggc gctcctggac gatcttcgtc
      241 gatttttaaa aatgttgcgt cacgcgcgta acgtgacagg gttaatatca caaagcaacg
      301 ccacttcacc aattgtgtaa agcgccatcg tctcaccctt gctcgcgagg tcccggttta
      361 actttagacg cagttttgcg aaccaggtag ttttgcccgt tttttgtgca tttatagggt
      421 gattttattt ttgccaggcg attttgagtg atcgtactca cgaattctca tttttctgca
      481 agagttcaaa gaaagttaaa cgcaggcaat gtatgttacg cgttttaaag ggatgtgtgg
      541 tttgcgggta tgtacgattt taatctggtg ttgctgctgc ttcagcagat gtgcgttttt
      601 ttagtcattg cgtggttaat gagtaaaacg ccattattca taccgttaat gcaggtcacg
      661 gttcgtctgc cgcataaatt tctctgctac atcgtctttt ccatcttctg catcatgggc
      721 acctggtttg ggttgcacat tgacgattct attgccaata cccgtgcgat aggcgcggta
      781 atgggcggct tactcggcgg tccggtcgtc ggtgggctgg ttggcctgac cggcgggtta
      841 catcgatatt cgatgggggg catgaccgcg ctaagttgca tgatctcgac catcgttgaa
      901 ggattgctcg gcggcctggt acacagcatc ctgatccgtc gcgggcgcac tgataaagtc
      961 tttaacccca ttaccgctgg tgccgtcacg ttcgtcgctg aaatggtgca aatgctgatc
     1021 atcctggcga tcgcccgacc ttatgaagat gcggtgcgtc tggtgagtaa tattgctgcg
     1081 ccaatgatgg tcaccaatac cgtcggcgcg gcgctgttta tgcgtatatt gctcgataaa
     1141 cgcgcgatgt ttgaaaaata cacttcggct ttttctgcca ctgcgctgaa agtggcagcc
     1201 tcgacggaag gcattttgcg ccaggggttt aacgaagtga acagcatgaa agtggctcag
     1261 gtgctgtatc tggagctgga tattggtgca gtcgcgatta ccgatcgaga gaaattgctg
     1321 gcctttaccg gaattggtga cgaccaccat ttacccggca aaccgatttc ttcaacttat
     1381 actttaaaag cgattgaaac cggtgaagtg gtctacgctg atggcaacga agtaccttat
     1441 cgttgctctt tgcatccgca atgcaaactg gggtcgacgc tggtaattcc gttgcgtggt
     1501 gaaaatcagc gggtgatggg caccatcaaa ttgtatgaag ccaaaaaccg tttatttagt
     1561 tcaatcaacc gcacgctggg cgaggggatt gcgcaactgc tttcggcgca gatcctcgcc
     1621 gggcaatatg agcggcaaaa agcgatgctc acccagtcag agatcaaact gcttcacgcc
     1681 caggtgaacc cccatttttt gtttaatgcg cttaacacca ttaaagcggt gatccgtcgc
     1741 gacagcgaac aggccagcca gctggtgcag tatctttcca cttttttccg caaaaactta
     1801 aagcggcctt cggagtttgt tactctcgcc gacgaaattg aacatgtgaa tgcttatctg
     1861 caaattgaaa aggcgcgctt ccagtcgcgg ttgcaggtca acattgctat tccgcaagaa
     1921 ttatcccagc agcaattgcc cgcgtttacc ctgcaaccga tagtggaaaa cgccattaaa
     1981 catgggacat cacaactgct ggatacaggg cgagtggcaa tcagcgtccg acgtgagggg
     2041 caacatttga tgctggagat cgaagacaat gccggtttgt atcaaccggt aaccaatgcc
     2101 agtgggctgg ggatgaatct ggtggataag cgtttacgtg aacggtttgg cgatgactat
     2161 gggataagcg tcgcctgtga gcctgatagt tacacccgaa taacgttacg actaccatgg
     2221 agggacgagg catgattaaa gtcttaattg tcgatgatga accgttagca cgggagaacc
     2281 tgcgcgtatt tttgcaggag cagagcgata ttgaaatcgt tggtgagtgt tcaaacgccg
     2341 tggaagggat cggcgcggtg cataaactgc gcccggatgt gctgtttctc gatatccaga
     2401 tgccgcgcat cagtggtctg gaaatggtgg ggatgctcga cccggaacat cgtccgtata
     2461 ttgtttttct caccgcgttt gacgaatacg ctattaaagc ctttgaagaa catgcctttg
     2521 actatctgct gaagccaatt gatgaagcgc gactggagaa aacgctggcg cgattgcgtc
     2581 aggagcgcag caagcaggat gtttcgctgt taccggaaaa tcaacaggcg ctgaaattta
     2641 tcccttgtac ggggcatagt cggatttatt tgctgcaaat gaaagatgtg gcatttgtca
     2701 gcagtcggat gagcggtgtc tacgttacca gccacgaagg gaaagagggc tttaccgaat
     2761 tgacattacg taccctggaa agtcgtacac cactactgcg ctgccatcgt cagtatctgg
     2821 ttaacctcgc gcatttacag gagattcgtc tggaagataa cggtcaggcc gagttgattt
     2881 tgcgtaatgg cttaaccgtg ccggtcagcc gccgttatct gaaaagctta aaagaggcga
     2941 ttggcctgta aaagactgct aaaatggctt tttgcctcat caacacctga aggcctcatg
     3001 ctaagtaacg atattctgcg cagcgtgcgc tacattttga aagccaataa taatgacctg
     3061 gtgcgtatac tggcgctggg taatgtcgaa gccaccgcgg aacagatcgc cgtctggcta
     3121 cgtaaagaag acgaagaggg ttttcagcgt tgtccggaca ttgttttgtc gtcattcctc
     3181 aatggcctga tttatgaaaa acgcggcaag gatgagtctg ctccggcact ggagccggaa
     3241 cgtcgcatta ataacaacat cgtgctgaaa aaattacgca tcgcgttttc gctgaaaacc
     3301 gatgacattc tggcgatcct caccgaacag cagttccgcg tttcgatgcc ggaaattacg
     3361 gcgatgatgc gtgcaccgga tcataaaaac ttccgcgaat gcggcgatca atttttacgt
     3421 tattttctgc gtggactggc agcgcgccag catgtgaaga aaagctaaga cgggtatggc
     3481 ggccatgcgc aaacatggcc gccgacagat tatttcactt ctttaaaacc agcggctttc
     3541 atcaccagtt ccatttgcgc catagtgata ccttttttgg catcttcagc agaaacgttg
     3601 attcctgaaa taccctgcag ggctttaaaa tccacttttt ccatatcgat agtcacgttt
     3661 tcctgcgcgt aggtatcggt ataggttaat ttttcttcaa cacccgcgat gtttttgtat
     3721 ttggcgctta acggctcaag tgtcttggca gcatcttctt tggtggtagc accaatggag
     3781 gcaaattgaa ttttggtttc agaagattgc ttaagcacct tgtcaccttt gtagacatag
     3841 gtaatggcaa tttcagtgcc gttcagattg gcgctgaatt tcttcgattc ttctttgtca
     3901 ccgcagccag caagagaaaa aaccagaaca gatgcaacaa caagggaaaa cagcttattg
     3961 aaagccttca tgtaaaactc cattttattt aatcaaggaa ctggtgactc tcaccagggg
     4021 ctatatagga tatgcctaat accgtggcgt gagcagtccg gaactggagt agaactctta
     4081 gtaaaaagca ctatttcatc cttgttgctg aagcaggggg aataattgtt cgcacagcaa
     4141 aacaccgtta ttcattgctt ctacccgtgc ctcgctttct gtattacgaa attgtcccaa
     4201 tacatgtgcc agccgataaa aacccaccgc ggtgaggtca ttcgccagca actctgcctg
     4261 accaatagca ctctgttcct gatagcgcca gccgttatgg agcagttgaa taagtaatgc
     4321 ctggcagcgc atcagcaact gatgagcagt agacggcaca ggcagaacgc tggcagaagg
     4381 tagtgatgcc accacaggcg cagtttctgc gtccagcgcc caggcacgag tctttgtcat
     4441 cattacctgt ggttccagtg tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag
     4501 acacacgggg tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg cataaggcgc
     4561 gctggctgag gctggcagtg ataacgtcag cagattatct tcaccttcgc cgctgattac
     4621 ctgcgcatcc agcgtctggc ggctgctatc ccaaccgagc gaaatacact cagcgaccgg
     4681 cagaataaat aagttatcga cctgattaag aggccgtatg caggaggggg gacgctggcg
     4741 taaatattcc cgcaaagcca caatgcccgg ctggcgtaac ggcgcgctca acattcgcca
     4801 ggcatcaggc gacagcggca caacgctgct taagcggttg cgggtagcta acagcagctc
     4861 gccatcggca ctgcgttttg ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc
     4921 ctgaaaacta aacagccgac gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca
     4981 actgcgcgaa aggtgcaaaa tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc
     5041 ataatgctca atatcctgcc agcaacgcat acctaaagac accagccgca gatgatcaag
     5101 ctttgcttct ccggcaatgc cagagccaac gaccgtgcgc cacggcacag gaggaacttc
     5161 accaacactg tcgcgccggg ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc
     5221 aagctgacgt aagcattctc ctgcatgata gtggctggcg cgggcgtgga aggcatcaac
     5281 gctggcgcgc agttgccgta gcgattcact cacccagcgc cagttgcagc gttccgccgc
     5341 ttgctgcgcg cggctgaaag cggcctcgta gtgaataagc ggctggctga tgccgccaag
     5401 ccataatgcc tggcttaatt gctgaacata ttgacgacac gcgttgcctt cttcgctggc
     5461 aaacgggtcg tcagatgatg tgacgtgttc gctgcgcatc tgccagatta aatgagtaaa
     5521 ttccgcttgc tgagttttgg cctcgacgaa ggcctgcaca gccagtacga catgttcgca
     5581 aagtgtgcct tcaatacaat cacagcgggc gaagcgaatg ctgctgcggg aataaaaacg
     5641 cacatcactc atcggtaagc gggctgaggg aatttcgcca ggcgcacaga acaactcaat
     5701 ggtgatgcct ttagcgacca gcacctgtgc acgtttgcgg gtggcatcgg gcagggtagc
     5761 cagttcttcc agccagattg caggctgcca ttcttcttct ttttccgtgg gccgggtggt
     5821 ggcacaaagt cgctgataac ttaacaccag catcacgcga tgacggcaca tgccgctagc
     5881 cccgcaggtg cactgagcct ctttcagtgc ctggccgttc gccagctggg tacggacacc
     5941 gtcactgaag gtggcgatta aagcgccgtt ctcatggctg atttccggga cgttgccatt
     6001 ttccagttcc ttaaggctgc gcttaacaaa accggcattg cttaacgccg tcagggcctg
     6061 cggcgtgagt tctaataatt ccggacgtag tgaattcatg actgcaaatt ctccgcaagc
     6121 caggtagcca gttcacccgg tgtcattgcg gctatttgtg cgccaacatt aaccagcgcc
     6181 tgggccatat cgcggtcata gcaaggcgtt gcggtgctgt cgagcgctgc cagccccagc
     6241 actttgatgc cgctctggac acactttttc acctgatgcg tcagcaatga tgatgaaccc
     6301 ccttcataaa aatcactcac gaggataatg acgctttttg ctggttgttc aataagttgc
     6361 cgcccatact ccacggcact ggcgatattg gtcccgccgc ccaactgtac tttcattaat
     6421 aactctaccg gatcggcaac gtctgccgtg agatcaacga cactggtgtc aaacgccacc
     6481 agatgggtac gaatgccggg taactgccac aaacaggccg ccattaccgc agagtggatc
     6541 accgaatcga ccatcgatcc gcttagatca accagtaaga ccagttgcca ttgttcactg
     6601 tggcgcttaa ttcggctgtt aaagcggggg gattcgatat acaacttgcc gtgttgcggg
     6661 tgccagtgtt gcagattggc gcgaagagta cttttgaaat caaagtctcg cgccagtgga
     6721 atagatgagc ggcgacggcg atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc
     6781 agtcgagcca taatttcttc aacaacctgg cgcactatcc ggcgggcggc agccagtact
     6841 tcggggttca tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc agaaggctgc
     6901 atacgttcca gcacgtcgag attcgtcacc acatcttcaa tgccgtagcg cagtacggca
     6961 tcgctttcca gccgctcaat cacctgttgc ggaaacagcg tgtgaatact gttgatccac
     7021 tcaggggggg tgagatttga gccacctaat ccaccggagc gttcaccacg ctggagccgt
     7081 tcaggatcgc gcccatacag ccactccagc gcgtggtcta tctgccgggc gttgtcatcc
     7141 agcccacaaa gcgtcgtttc tgccgcttcg ccaagaatta atcgccagcg ttgtagctca
     7201 cgggtggtca gaagatcgtt cagttcagac atggaaaact ccccagtttt gcagcgatgc
     7261 cagtgcctgc tgttcgagtt gttgatgatg tgcaatggct tgtggtgggc aatgcaacgg
     7321 catttgcagc gccgaaacgg gaagttgcgc cagttgataa tgttcaagca cctgatgcgc
     7381 cagtgtcccg cgttctcgtg gcggtagcca ggccatcgcc gcgcgtaaat cgggcagggc
     7441 attgataaaa tcggcatcac ttggttgatt taaatgactg ctgaaaccgg cgataaatgc
     7501 cggctgacag gccagttgat ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga
     7561 ttgtgccggg gataactgcg ccagcatcgt cagcgccgct tcggcactag cgttgggatg
     7621 ttccagacgc atcagtgcgc caagagccgc gccgcgatcc agcgccgggg catggattgc
     7681 ctgactgcgt cgctccagta aggctaccgc cgcagaaaga gaaacgccgg gtaaattaac
     7741 gccgctatgt agatcgcgca gaatatggca aagcgcttgc cagctatgca ggtgagcgtg
     7801 aaactccttt tcatccggtc tgccgttaga ttcacacagc cacagcgtgc gatcgataac
     7861 cgcgcataac gtcgtttgtg atatctgcgc accttgcata ccgctaattt catccagccg
     7921 ccataaggca tatagcactt ccagcgccgg gcccatttcg gcaaattgat tttcctaggc
     7981 gattaataat gtgagttgct ccagtaattg ttgactgaag gacgccagac ccgctaacgc
     8041 cgcctggcta agacatgtgg tgatactgcc gattccaccc gcgtccagca tatcggcttc
     8101 taatttatgg cgtgcggctt cctggagtgt ggcaccaaag caggcggcct caatcaatgc
     8161 cgcatgctgg ctaagcgggc gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt
     8221 cagtgtgctt ccctgctggc gtacaatccc tgggatctcc agtattgcca gccgatgtaa
     8281 cacctgactt tgcgctagtc cattggggtt aaagcgattt aaggtaagct ccgccgaaag
     8341 agagattgcg gtacgttcca gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt
     8401 agaaggggca agtttaccga atccgtcacc cgctaatgtg tctatcaacg ttagcagaat
     8461 cggatcggta tcgggatgaa taacgccgcg atagctccac ggcaacggcg cgttcagggc
     8521 ttctttaatc agcgagcctg ctatggcatc cagccagtca gtgcgtaacg gtagtttata
     8581 tccgcgcaac tgtgccagcg ccatcgcatg cagatgagcg gcagccatat ccgcggtcga
     8641 agcaggcaag tggtgttggc gcaaacgggt aagaaccgtt tttagcagtt gttctcccgc
     8701 ctgctgtaag ccccactgcc agcaccagct ttgccagacc ggggcaggca ttcctgaaag
     8761 gtatcctgcc agcacatcaa ggcgcttttc actgtagggt gtgagataac aacctgtaac
     8821 ggcatctgcc agcgagggca attctggctt gttaatgtcc tgcgggcatt cgcgccacat
     8881 ttttgccagt gccggagcgt gccagccacc gcagacgact aacacgtcgc cattattttg
     8941 ctgcactgcc catgcaatcc agcgagccat aaaggcttcg cgctgacgat tgagtgcatc
     9001 gccggggaaa tcgccccgca gttgggcaaa atagtgcgcc agtgcagagg gtaatgcagt
     9061 ttgctggctt tcatcttcga acaagtgatc ccacagggta tcgctgttat ccatgcgggt
     9121 ggcacgcagc agcaaggcct ggctttcctc ttgcgtatca ggcgaatcgt cctcttcttc
     9181 actctgcgcc cagcaaggca aatcgataaa gtaagtttgt gcctgaatat gacgtgcggc
     9241 ttgtagcgcc tgccactccg gcgaaaattc ggcaaatggc gtccaggcgc cgcgcccagg
     9301 agcagcaccg tcctgatact ggcaataact gtaaatagcc accggaagct ggtgggctaa
     9361 aaacagttca tctacccggt cattaaaatc agccgggcct tcaatcaaca cgtatcgtgg
     9421 tcgctggctt tcgattaacg atttcaccag tcgggcgcag gccggactat gatgccggat
     9481 gccgacaatt aacggctcgc tcatgcatct ttcctcacgg caggcggtgg cgggcttgat
     9541 aataagcctg ccagtgagct tctttatgtg tggcgacacg ctgttcgaag taacggcgca
     9601 gacgagcgcg atcttcttcg ttatctttga cgatggttcc ggcaatacag tccaccaggt
     9661 cggcaggctc ccccgcgcga ttcgctaaaa accaggcgcg gacgcccaca gcgtgggcca
     9721 cgttcacggc ttcagcggtg gacataatcg ctgttagtgt atccattgac gttttctttt
     9781 cgccgttggc acgcagatcg cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca
     9841 ctttatgcgg aataccgcta tgggccagca aacgcgccga agcactggcg acaagctcca
     9901 gctcctgggc gaaatccata atcggaaaga cagtttcaaa atcgaagcga cgctttagcg
     9961 cagcactcat ttcattgacg ccgcgatcgc gggtattggc agtggcaata atattaaagc
    10021 cttctcgcgc atagagctga ctggcttcac cagtgagttc cggcaccgtc atcaccctgt
    10081 cagaaagcat acctaacaga cagtcttgca cttccagcgg tgtacgcgtt atctcttcaa
    10141 aacggacgat tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc agcgcttccg
    10201 ttgatggacc atgattgata agcagggcgt agttccagcc gtacttgatt tgatcttcgg
    10261 tagtagatgc gccgccctgg atggttaacc cggcatcgcc gctaatagag gttgccagta
    10321 attcagacag gagagatttt gcggtgccag gttcccccac caacatcaaa ccgcgtccgg
    10381 tggcaagagt gactaacata cgttcgacag cggagggact aatcaccact ttacggctaa
    10441 tattttgcgc gctatcgcca aggataaaag cacgggccgc aggcaaactt aactgccaac
    10501 cgggcgggca aggtgcgtta tcattttgtt ttaattttgc cagttcatcg gcgtataaca
    10561 cagcagcagg cggacgctgc agatgattat tctgcggtga catatattca tccctgaaat
    10621 gtattaatga aataaacgat attttacgcg caataaatgt ttggcgcatt agttgtttga
    10681 gcgaaaaata tctgggaagc agatcacagt aaataaaaaa acctgctggt gaggcaggtt
    10741 cttttattta ctattttcag gcctaacacc acagcctgtg caacatcgtg ttccaacgaa
    10801 cgaattataa ccacggctgg accccgttgc aattaagata atgagattta taatcctgac
    10861 tgttgggacg ttgcgccgga tcgaacctat ttcaagtcct ggctaccgga cggggggtgc
    10921 tgcaggtggt gccctgatcc ggagtgcaac atcgtggtcc aacatacgta aaccagtatc
    10981 gacaggcagt gtaagggtaa ggtgctggtg ttgcgctcct taataaggag agcggatgga
    11041 ctcgctgaca caaaagttaa ccgtgctcat tgctgtactg gagctattag tagctctgtt
    11101 acggttgatt gatttgt
//
LOCUS       DBFIFS010000149        11000 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.149, whole genome shotgun sequence.
ACCESSION   DBFIFS010000149 DBFIFS010000000
VERSION     DBFIFS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.149"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            13..138
                     /gene="yahH"
                     /locus_tag="N7A07_003387"
                     /pseudo
     CDS             13..138
                     /gene="yahH"
                     /locus_tag="N7A07_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            188..1138
                     /gene="yahI"
                     /locus_tag="N7A07_003388"
     CDS             188..1138
                     /gene="yahI"
                     /locus_tag="N7A07_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU2477988.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1148..2530
                     /gene="yahJ"
                     /locus_tag="N7A07_003389"
     CDS             1148..2530
                     /gene="yahJ"
                     /locus_tag="N7A07_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU2477989.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2829..3239
                     /locus_tag="N7A07_003390"
     CDS             2829..3239
                     /locus_tag="N7A07_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU2477990.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3493..4476
                     /locus_tag="N7A07_003391"
     CDS             3493..4476
                     /locus_tag="N7A07_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2477991.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4525..6009
                     /locus_tag="N7A07_003392"
     CDS             4525..6009
                     /locus_tag="N7A07_003392"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2477992.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            6002..6973
                     /locus_tag="N7A07_003393"
     CDS             6002..6973
                     /locus_tag="N7A07_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477993.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            6970..7926
                     /locus_tag="N7A07_003394"
     CDS             6970..7926
                     /locus_tag="N7A07_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2477994.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8013..9062
                     /gene="yahK"
                     /locus_tag="N7A07_003395"
     CDS             8013..9062
                     /gene="yahK"
                     /locus_tag="N7A07_003395"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU2477995.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9305..10120
                     /gene="yahL"
                     /locus_tag="N7A07_003396"
     CDS             9305..10120
                     /gene="yahL"
                     /locus_tag="N7A07_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU2477996.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10572..10781
                     /locus_tag="N7A07_003397"
     CDS             10572..10781
                     /locus_tag="N7A07_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2477997.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10798..>11000)
                     /locus_tag="N7A07_003398"
     CDS             complement(10798..>11000)
                     /locus_tag="N7A07_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU2477998.1"
                     /translation="PTWARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVAS
                     RVIGAIIGVFALRLIYEGVTQR"
BASE COUNT         2728 a         2509 c         2931 g         2832 t
ORIGIN      
        1 aatggcacga gaattgtagg cctgataagc gtagcgcatc aggcatttgt caccattgcc
       61 ggatgcggcg tgaacgcctt atccggccta cgaatggcac gagaatttgt aggcctggta
      121 agcgtagcgc atcaggcagt ctggcgttgg tcataacccc atcaccctct gtagcggaca
      181 taacaacatg aaagaacttg tggtcgttgc cattggtggc aacagcatta tcaaagataa
      241 cgccagccag tcgattgagc atcaggcgga ggcggtgaaa gccgtggccg atacggtgct
      301 ggaaatgctg gcttccgatt acgacattgt gctgacccac ggtaacgggc cgcaggtcgg
      361 gctggattta cgccgtgcgg agattgccca cgagcgcgaa gggctgccct taacgccgct
      421 ggcgaactgt gtggcagata cgcagggcgg cattggctac ctgatccaac aggcgctgaa
      481 caaccggctg gcgcgtcacg gcgagaaaaa agccgtcacc gtggtgactc aggtggaagt
      541 ggataaaaac gatccaggtt ttgcccatcc caccaagccc attggtgcat tcttcagtga
      601 aagccagcgt gacgaattac aaaaggcaaa ccctgactgg cgttttgttg aagatgctgg
      661 acggggctat cgccgcgtgg tcgcctcgcc ggaaccgaaa cgtattgtcg aagcacccgc
      721 cattaaggcg ctgatccaac aaggctttgt ggtgattggc gcgggcggcg gcggaattcc
      781 tgtggtgcgt actgacgcgg gagattacca aagcgtggac gcggttatcg acaaagatct
      841 ctctaccgcg ctgctggccc gtgaaattca cgccgacatt ctggtgatca ccaccggcgt
      901 cgaaaaagtc tgtatccact ttggtaaacc cgagaagcag gcgctggatc gggtggatat
      961 tgccaccatg actcgctata tgcaggaagg acatttcccg cccggcagca tgttgccaaa
     1021 aatcatcgcc agcctgacgt tcctggaaca gggcggtaaa gaagtgatta tcaccacgcc
     1081 ggaatgcctg ccagcggcgc tgcgcggtga aacgggcacc cacattatta aaacgtaagg
     1141 acgtaagatg aaagaaaata atagccgccg tgaatttctg agccagagcg gtaagatggt
     1201 caccgccgcc gcgctgtttg gtacctctgt gccgctcgcc catgcggcgg tctctggcac
     1261 cacaaactgc gaagcgaaca acaccatgaa aatcactgac ccgcattact atctcgacaa
     1321 cgtgctgctg gaaaccggtt ttgactacga aaatggcgtg gcggtgcaga cccgcacggc
     1381 gcgccagacc gtggagattc agaacggcaa aattgtcgcg ctgcgcgaga ataagcagca
     1441 cccggatgcc acgctgccgc actatgacgc tggcgataag ctgatgctgc ccaccacccg
     1501 cgacatgcat attcatctcg acaaaacgtt ttacggcggg ccgtggcgct cgctcaatcg
     1561 cccggcaggc accaccatcc aggacatgat caaactcgag cagaaaatgc tgccggagct
     1621 gcaaccgtac actcaggagc gggcagaaaa actgattgat ttattgcagt cgaaaggcac
     1681 caccattgcc cgcagccact gcaatatcga accggtttcc ggcctgaaaa atctgcaaaa
     1741 tttgcaggcg gtgctggcgc gacgtcaggc gggctttgag tgtgaaatcg tcgccttccc
     1801 gcagcacggt ttgctgctgt cgaaatcgga acccttaatg cgtgaagcaa tgcaggcggg
     1861 tgcgcattac gtcggcgggc tggacccgac cagtgtcgat ggcgcgatgg aaaaatccct
     1921 cgacaccatg ttccagattg cgctggacta cgacaaaggc gtcgatattc acctgcacga
     1981 aaccactccg tcgggcgtgg cagccatcaa ttatatggtt gaaacggtag agaaaacgcc
     2041 tcaactgaaa ggtaagctga ccatcagcca cgcctttgcg ctggctacgc tcaacgagca
     2101 acaggtagat gaactggcgc atcgcatggc ggcgcagcaa atttctatcg cctcgacggt
     2161 gccgattggc acgctgcata tgccgctcaa acagttgcac gacaaaggcg taaaagtgat
     2221 gactggcact gacagcgtta tcgaccactg gtcgccttat ggtctgggcg acatgctgga
     2281 aaaagccaat ctctacgcgc agctctatat tcgtcctaac gaacagaacc tctcccgttc
     2341 gctgtttcta gccactggcg atgtattgcc gctgaatgaa aaaggcgagc gtgtatggcc
     2401 aaaagcgcag gatgacgcca gctttgtgct ggtggacgcc tcctgttccg ccgaggcggt
     2461 ggcgcgtatc tcgccgagaa ccgcaacgtt ccataaaggg caactggtgt gggggagtgt
     2521 ggcaggttga tgcgcatttg aaacgcctgc aacggtgaat agtaagagat ttaagcccca
     2581 gggagtgggg cgggaatggt ttgctaatga atgatcctga actttataat ctcccggcat
     2641 aatttccagg ggattatttg agagaaatac acaaaagtga gttttaacaa aggtatttat
     2701 caggccataa atgtgacctg cttcggataa aaaagattca gtatcgaaaa accaatgata
     2761 ctcttgctga tggttaccaa cagtaattaa gatgtgattt gtttttccgt aatcgcaaaa
     2821 atattgccat gaccataaaa gaaaaattct cacaaaaata tccccatgct tccttttgta
     2881 cttttggcga ttcagccgcg ttggccgatc acctcgcaac gttgattgcg actggtgtta
     2941 aaacagcttc ctgtggttcg ctggcgggat gtattgaaga caacgcattt ccgatgattg
     3001 gtgagtataa gattgtagaa aatagccgtg gtgagcctgt atgtgttatc agagtgattg
     3061 gcctgcattt actgcgcttt tctgatgtca ccgcggagct tgcccgtaag gaaggtgaag
     3121 gtgacctcag tcttgaatat tggcgtaatg aacaccgtcg ttttttccag gcagaaggaa
     3181 gctattcacc agaaatggac gttatctttg aagaatatgc ccttattgac gttgtgtaga
     3241 taactattta tctacatgaa ataagctcac aaacataaaa tatcgctaat tttgtttatt
     3301 ttgctgtttt ttacgtgatg aatatcacac ttattattgc tgcccgtttt gtccatattt
     3361 ataaccatat ttttaatagc tgcctgaaat agagctgttt attctgcata aaaatattcg
     3421 aaaattctta ttctctccag cgcgctaatt tgggcgaaca cttcctgact accctgcaat
     3481 gaggctgaaa tgatgaataa acgttttgtt atcaatatgg tgtcttcgct gttattaggc
     3541 gctgcgctaa tttctgcgcc gctccaggca gcggaaaaag tcgtggtgaa tatttctaaa
     3601 gtcgatggta tgccgtggtt taatcgcatg ggcgaaggtg tggttgaggc gggtaaagcc
     3661 tttggtgtta atgcctcaca ggtggggcct tcaagtactg atgctccgca acaagttaaa
     3721 attattgagg atttaatcgc ccgtaaggtt aatgcaatta ctatcgtgcc aaacgatgcc
     3781 aacgtgctgg aaccggtatt caaaaaagcg cgagatgccg gaattgtggt tctgaccaac
     3841 gaatcccccg gtcagccaag tgctaactgg gatatcgaaa tcattgataa cgaaaaattt
     3901 gccgctgaat atgttgagca tatggcgaaa cgcatgggtg gcaagggcgg ctatgtgatt
     3961 tacgtgggta gtttgaccgt gccgcagcat aacttatggg ctgacttgct ggtgaaatac
     4021 cagaaagagc attacccgga tatgcatgaa gttacccgcc gcatgccggt tgccgagagt
     4081 gtggatgtct cacgtcgtac tacactggac ctgatgaaaa cctatcctga cttgaaagcg
     4141 gttgtctcct ttggttcaaa cggtccgatt ggcgcgggac gtgcggtgaa agagaaacgg
     4201 gcgaaaaata aagtcgcagt ctatggcatg atgatccctt cacaggcggc atcactgatt
     4261 aaaagtggtg atatcaccga agggattact tacgatccgg cctctgcagg ttacgcactc
     4321 gcagccgtcg ccagcacgtt gctgaagggt gaagagatca agcctggatt agaaatgcag
     4381 aatctgggta aggccgacgt agatatggat aagcgaatta tccgtttcca taaggtatta
     4441 ctggtcaaca aagacaatat cgactcactt tattaatcca tttcttcggg gcgaaagccc
     4501 cgattcttat tgccagggga tgttatggaa accttccttt cccttcgtca tatcaacaaa
     4561 acgtttcatg ccacgcgagc gctgcgcgat gtttctctcg actttatgtc gggtgaagtg
     4621 cactgccttg ccgggcaaaa cggctgtggt aaatcgacgt tgattaaaat tatgtccggt
     4681 gtttatcgcc cggatgaggg agcggaaatt acgcttggtg ggaaaaactg gtcaaagctg
     4741 acacccgccg cttcggtggc gcaggggatt caggtgattt atcaggacct ctctttattt
     4801 cctaacctga gtgtctggga gaatatcgcc gtgaatcact atcaccacgg tctgtttgtt
     4861 aaccgccgtc gtctgcgtga ggtggcgcag gcggcaatga ctagtattaa cgtcacattg
     4921 ccactggata cgctcgtttc tgagctttca attgcccgct gtcagctggt ggcaatttgc
     4981 cgtgcactgg cgcaggacgc acggttgatc gtgatggatg aaccaaccgc ttcgctgacc
     5041 catcaggaag tgcaagggtt attacaggtg gtgcatcaat tgcgtgaacg cgggatctgc
     5101 gtggtctttg tcagtcatcg tctggaagaa gtgatggaag tttccgaccg tatttcagtg
     5161 ctgaaagatg gtgagctggt cgggactttt ccggctgcag agatgaccac aaaacaactc
     5221 ggtttcctga tgaccggcca ggagtttgaa tatcaggtgc gagagttgtg gcagggaaaa
     5281 tccagcaccc cggtgctgga ggtgcgtaat ttaagtcgtc atggggaata tctaaatatt
     5341 aacttgcggg tggaagcggg cgaagtggta tcgattgttg gcctgcttgg tgcggggcgc
     5401 acggaattat gtctgagtct gtttggtatg acccgaccag atgctggcga gatcctcatc
     5461 aacggccagc tggttacact gcatagtaac caggacgcca tccgccacgg tattggttat
     5521 gtttcggaag atcgcatgtc gcgcggtttg gtgatggcgc aatctattga agacaacatc
     5581 attagcactg tttttcacaa ggtaaaagat cgcttcggct ttctgagtga aactaaagtg
     5641 cgtgacctgg tggacaggct aatcaaggca ttgaccatca aagtctctga tcctcatctt
     5701 ccggtgaata cgctttctgg tggtaacgcg cagcgagtat cgatcgccaa atggctggcg
     5761 attgggccac gactgttgat tctcgatagc ccgacagtcg gtgttgatat cgccaacaaa
     5821 gcaggaatat acggcattat cagcgatctg gcggctcatg gcattgcggt attgatgatc
     5881 tgcgatgaaa ttgaagaagc gtggtatcaa agccaccgga ttttggtaat gcaaaaaggt
     5941 cagatcaccc atagcttcct gcctgacagc agttctcagg ccaggattgc ggaggtggta
     6001 aatggctgaa ctgaaaaaac gccatgaatt ctggctggca ttactgattg tggtgctgtt
     6061 tgttggcctt gcctggcgca gcgacgagtt tctgacattc ggtaatttgt acgatctcgc
     6121 caataactat gccatgttga ctattctcgc ctgtggtttg tttgtggtgt tgatttccgg
     6181 tggaattgat atttcgtttc cagcaatgac catcattgcg caatacggca tggtgctgtt
     6241 gctgcaaaaa attggtggca acttcgctgt cgcgtttgca ctggcaggtt gcatcggcat
     6301 tttacttggc ttaattaacg ccttactggt taatcgccta cgggtgcctt ctatcatcat
     6361 cactatctcg acgctgaata ttttctatgg cctgctgtta tggttgagta aaggtgtgtg
     6421 gctgtacgac tttccgccgt ggtttgagca gggggttatg ttgttcaagt acaccgatgc
     6481 tgatggctat gactatggcc ttggtctgcc gctgatcgcc atgattacgg tggtgctgct
     6541 gacagcgttt atcatgaatt tcaccagtgt agggcgcaaa atttatgccc ttggcgggaa
     6601 ccgcgaatca gccagtcgca tcggctttag cgtgctgaaa ctgcaacttt tcgtctacgg
     6661 ctatatggga ttgatgtctg gcgctgcggg tgtagtgcag tcgtggacgg tgatgactgt
     6721 cgcccccgat tctcttctgg gttatgagct gacagtactg gctgcggtgg tgcttggcgg
     6781 cactagtttg ctcggcgggc gcggcacgtt aactggtact ttgctcggcg tggtgttgtt
     6841 ggcagtgatg caaaacgggc taaatttatt gggagtctcg tcttactggc aaacattgat
     6901 caccggcatc atcatcgttg ccagcattag cgccacggcg tggagtcagc atcagaaccg
     6961 gagcctgcta tgaaaaaatc gtggcgtaat aacgttgagt tttacttgat cggcctgctg
     7021 gtgctaactg ttgccgcgtt cagcattact atgccggaga ttttctggtc gataagtaat
     7081 ttccagtccg tcgcttcgca aatgcccgtg ctgggcattc tggcgttggc tatggcggtg
     7141 accatgcttt gcggtggcat taacttgtca attatagcca ctgctaacgc ctgttcactg
     7201 gtgatggcgt gggtggcgac tcagtatcca ccgggtattg ctacggttgt cgctacgtta
     7261 ctggcgggcg ctggcgcggc ggtgataatc ggtttatgta acggcgtatt gattgctggt
     7321 attcgagtgt tgccaattct tgcaacgctt gggatgatga cgttgctcaa agggataaat
     7381 attctggtga caggtggtag cgccattgcc aattatccct catgggtgct gtggttgaat
     7441 catgcgcaat ggttcggtat tccgctaccg atgtggttgt ttgccgcagt ggcactgggg
     7501 ctgtggatat tgctggagaa aacgccgctc ggtaaagcca tttacctgat tgggtctaac
     7561 gaacgagcaa cactttactc cggcattaac actcgtcggg tgctgatttg ggtttatgtc
     7621 atttccgcct tgctttgtgc ggtagcggca tttttgatga tgtcgaaact caactcggct
     7681 aaggcctctt atggcgaatc gtacttgctg gtgtcaatcc tcgcggcagt actcggcggg
     7741 gttaatccgg acggcggcag cgggcggata attggtatgg tgctggcgct gtttcttctg
     7801 caaattattg aaagcggctt caatattctc ggtataagcc cctacctgac gatggcctta
     7861 tggggaacgt tgctgctctg tttcattcag gctcgcggca tgttggggct ggatcgggtg
     7921 gtttaaatat tggctatatt caatggacgc gttttgccgc gatgacatat caggcgtcgc
     7981 caaatacaca tagctaatca ggagtaaaca caatgaagat caaagctgtt ggttcatatt
     8041 ccgctaaaca accacttgaa ccgatggata tcacccggcg tgaaccggga ccgaatgatg
     8101 tcaaaatcga aatcgcttac tgtggcgtct gccattccga tctccaccag gtccgttccg
     8161 agtgggcggg gacggtttac ccctgcgtgc cgggtcatga aattgtgggg cgtgtggtag
     8221 ccgttggtga tcaggtagaa aaacatgcgc cgggcgattt ggtcggtgtc ggctgcattg
     8281 tcgacagttg taaacattgc gaagagtgtg aagacgggct ggaaaactac tgtgatcaca
     8341 tgaccggcac ctataactcg ccgacgccgg acgaaccggg ccatactctg ggcggctact
     8401 cacaacagat cgtcgttcat gagcgatatg ttctgcgtat tcgtcacccg caagagcagc
     8461 tggcggcggt ggctcctttg ttgtgtgcag ggatcaccac gtattcgccg ctacgtcact
     8521 ggcaggccgg gccgggtaaa aaagtgggcg tggtcggcat cggcggtctg ggacatatgg
     8581 ggattaagct ggcccacgcg atgggggcac atgtggtggc atttaccact tctgaggcaa
     8641 aacgcgaagc ggcaaaagcc ctgggggccg atgaagttgt taactcacgc aatgccgatg
     8701 agatggcggc tcatctgaag agtttcgatt tcattttgaa tacagtagct gcgccacata
     8761 atctcgacga ttttaccacc ttgctgaagc gtgatggcac catgacgctg gttggtgcgc
     8821 ctgcgacacc gcataaatcg ccggaagttt tcaacctgat catgaaacgc cgtgcgatag
     8881 ccggttctat gattggcggc attccagaaa ctcaggagat gctcgatttt tgcgccgaac
     8941 atggcatcgt ggctgatata gagatgattc gggccgatca aattaatgaa gcctatgagc
     9001 gaatgctgcg tggtgatgtg aaatatcgtt ttgttatcga taatcgcaca ctaacagact
     9061 gaaaaaatta ataaatacgc tgtggtttaa catattaact tcgctctcca cttaactttt
     9121 tagttaagga gagcgaaata atatcaaagt agcagtaaaa cctataacgt aaattcaaat
     9181 tgttaaatta actccctcca gtacctaata cttcacacgt tagttatgag cgatttctga
     9241 tagtgcctgg tttaatcaga gctttattat ctgcgacgtt tatttttatt taagagagat
     9301 cgcgatgata tcattaaaag ctccgcacaa taatttgatg ccatatacgc aacaaagcat
     9361 acttaatacg gttaaaaaca atcagttacc agaggatatc aaaagctccc tggtttcctg
     9421 tgtcgatata ttcaaggttt tgattaaaca atattatgat tatccttatg attgtcgtga
     9481 tgatttagtc gatgacgaca aactcattca tctcatggct gccgtacgag actgtgagtg
     9541 gtcagacgat aacgcactca ccataaatgt gcagtttaat gatttccctg gattttatga
     9601 ctggatggat taccctgatc acccggttaa gtttgttttt cgcatacttg aaaatcaaaa
     9661 agggacagtt tgggtatatg accaggatga tgcgtttctt gatattaaag caaatgtgca
     9721 ggctggacgc tttaccgggc ttaagaaatt agtgcagttt atcaattcag tgcgaacaga
     9781 ttgcaaatgt attcttcttg aacaccatat gcctttgctg agacttttcc ctaaagggaa
     9841 agagtgtatg catgtggaaa aatggcttcg cgaaatgtcg tctattccgg agacagacgc
     9901 gcccattaag caggcgcttg cgcatggatt attattacac ttaaaaaata tctaccctgt
     9961 ttttcctgaa tcactggtga tgttattgct ctctgttctg gacgtaaaaa catacagaga
    10021 tgatgcacga ttaaatgaag ggatatctaa tagagtccag gaattaggtg acagatatta
    10081 tcctgtaaat aagcacgtta aaatccgcta cactctttag ttcagaaagg gttccacctg
    10141 caagttgtta ttctacctga gagtgaatcg caatgcaggt aatcatttca tctgtatatc
    10201 tgtatgccaa ctggcattcc atctcttttt tcgccgatat tttgttgtga tcggcgacac
    10261 ttcgcttaaa aaagcaccag tagtggtttc gcagccatgc ggtgtataaa aaaaaatgat
    10321 ctcatgcaga tgttttgtga atgtgtgggt tgacattcat atgaaaaaaa tcataattcc
    10381 atcatgtttg tgtggggtct tttctgtatc ttacgcatcg cactcaagcc tgacagaaaa
    10441 tatgctgtaa ggctcatatc aaaaaccgcc attagctcat caggaagagc agacgacaac
    10501 caggattgtt gtatggtacg gggttcgagg ccccgatggc ggtccaactt ttcaaaactc
    10561 ttctgaatta gatcccctta ttatcatccc tgcaaagtgg aacattgcca ttgtttggct
    10621 attcaggacg ggtcagacca atgaaaaaag cccacacagg ggagagtggg ctgaaatggg
    10681 aagctaaaga ctcaagtaaa cttatcggaa ataaggacca cgcattacgg gggctatcat
    10741 cgccgatggt cgggattcgg caaatcaggt tgattacgta gagagtaaat tatctgctca
    10801 ccgctgcgtc accccttcgt aaatcaggcg tagcgcgaat acaccaataa ttgcaccaat
    10861 aacccgactg gcaacgcgtt gcatacgccc ataagcacga cgcacagcgg gcaaagaaaa
    10921 cgcctgacta agaaaaactc gccagataat tgatgcgagc acaatccccg cccaggccat
    10981 taaacgtgcc catgttggtg
//
LOCUS       DBFIFS010000150        10931 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.150, whole genome shotgun sequence.
ACCESSION   DBFIFS010000150 DBFIFS010000000
VERSION     DBFIFS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.150"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            136..897
                     /gene="adiY"
                     /locus_tag="N7A07_003399"
     CDS             136..897
                     /gene="adiY"
                     /locus_tag="N7A07_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU2477999.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1034..2371
                     /gene="adiC"
                     /locus_tag="N7A07_003400"
     CDS             1034..2371
                     /gene="adiC"
                     /locus_tag="N7A07_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU2478000.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2475..4118
                     /gene="eptA"
                     /locus_tag="N7A07_003401"
     CDS             2475..4118
                     /gene="eptA"
                     /locus_tag="N7A07_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU2478001.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4115..4783
                     /gene="basR"
                     /locus_tag="N7A07_003402"
     CDS             4115..4783
                     /gene="basR"
                     /locus_tag="N7A07_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU2478002.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4784..5884
                     /gene="pmrB"
                     /locus_tag="N7A07_003403"
     CDS             4784..5884
                     /gene="pmrB"
                     /locus_tag="N7A07_003403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU2478003.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5860..5949)
                     /gene="pmrR"
                     /locus_tag="N7A07_003404"
     CDS             complement(5860..5949)
                     /gene="pmrR"
                     /locus_tag="N7A07_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU2478004.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6061..7563)
                     /gene="proP"
                     /locus_tag="N7A07_003405"
     CDS             complement(6061..7563)
                     /gene="proP"
                     /locus_tag="N7A07_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU2478005.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7827..8705)
                     /gene="rdcB"
                     /locus_tag="N7A07_003406"
     CDS             complement(7827..8705)
                     /gene="rdcB"
                     /locus_tag="N7A07_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU2478006.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8702..10930)
                     /gene="crfC"
                     /locus_tag="N7A07_003407"
     CDS             complement(8702..10930)
                     /gene="crfC"
                     /locus_tag="N7A07_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU2478007.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2737 a         2861 c         2691 g         2642 t
ORIGIN      
        1 ataaaaattt gtaatattag taaaactcgt tatttttatg catgtttata ttcatcatac
       61 aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct tttattcgca
      121 ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt attgactgaa
      181 aaagatgtct ggataagggt taatgggaaa gaacctatta gccttaaagc taaccatatg
      241 gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa cactttggtt
      301 gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa agatctctcg
      361 caaataccag tatggcaacg tagcgctacg cccattctca ccctgccatg cctgacacca
      421 gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga gtcagaaaag
      481 gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag taaaaaattc
      541 ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagcgttta tcaaattatt
      601 gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt atgtcttagc
      661 ccaagtctgt taaagaaaaa gctgaaaagc gaaaacacca gttatagcca aataatcacc
      721 acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag
      781 gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt taaagacttc
      841 tacggtatga caccgctgca ttatgttagt cagcacagag aacgcactgt cgcctgattt
      901 ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt cgataataat
      961 tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag ctcgccttcg
     1021 caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa tccccgtcac
     1081 cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg caaacctggc
     1141 ctccaccggc gggattgcca tttatggatg gttggtgacg attatcggtg cgctggggct
     1201 ctcgatggta tacgccaaaa tgtcgttcct cgacccaagt cctggtggtt cttacgctta
     1261 cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct actggctggc
     1321 ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa gttacttctt
     1381 cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc tgtggatctt
     1441 cgtcctgctg aacattgtcg gtccgaaaat gatcacccgt gtgcaggccg ttgccaccgt
     1501 gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt ttcgtggtga
     1561 aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcaa ttcaaagtac
     1621 ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt
     1681 ggtgaaaaac ccgaaacgca acgtccctat agccaccatt ggtggggtat tgattgccgc
     1741 cgtttgctat gtgctctcca ccaccgcgat tatggggatg atccctaatg ccgcactgcg
     1801 cgtttctgcc tcgccattcg gtgatgccgc acggatggcg ttgggtgaca ccgccggggc
     1861 catcgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct ggacattgct
     1921 ggcgggtcaa acggcgaaag cggctgccga tgacggactg ttcccaccga tttttgcccg
     1981 tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt tgatgaccat
     2041 cttccagctc agcagcattt caccaaacgc gaccaaagaa ttcggtctgg tttcttccgt
     2101 gtcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac tgctgctcgg
     2161 acacggtcac tttggtaaag cacgcccggc atatctggcg gttactacca ttgccttcct
     2221 ctactgcatc tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt catttgtcac
     2281 cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctgcata aaaacccgta
     2341 tcccttagat gcaccaataa gcaaagatta attcccctta atccagcaaa gataaaagcc
     2401 aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc actttgttcg
     2461 atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat ttactcgcct
     2521 ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt tttaaacagg
     2581 tgttgcaggc gctgccgctg gactcgctgc ataacgtact ggttttcttg tcgatgccgg
     2641 tcgtagcctt cagcgtgatt aatattgtcc tgacactaag ctctttctta tggcttaatc
     2701 gaccgctggc ctgcctgttt attctggttg gcgcggctgc acaatatttc ataatgactt
     2761 acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact ccggcagaaa
     2821 gctatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc gtgcttgctg
     2881 cgctgattgc ctgctggatt aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt
     2941 tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc gcactatttt
     3001 ataaagacta cgcctcgttg ttccgcaaca acaaagagct ggtgaagtcc ttaagcccct
     3061 ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca aatctgccgc
     3121 tggtgcgaat tggtgaagac gcgcaccgca acccattaat gcagaacgaa aaacgtaaaa
     3181 atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct
     3241 acccgcgtga aactaacccg cggttggcga aagataacgt ggtctatttc cctaataccg
     3301 catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat atgccgcgag
     3361 agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc attcagcgag
     3421 cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg
     3481 tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac ggcgaatgct
     3541 atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag ggtgatggcg
     3601 tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc tatccgccgc
     3661 agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt accaaagagc
     3721 aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt gataaagcga
     3781 ttaatctgct gaaagaacat caggataaat ttaccaccag cctggtttat ctttctgacc
     3841 acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat gccatcgccc
     3901 cggatagcca aaaacaggtg ccgatgctgc tgtggctgtc ggaggattat caaaaacggt
     3961 atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca
     4021 atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac caggctgcgg
     4081 atgatattct gcaaacttgc aggagagtga gtgaatgaaa attctgattg ttgaagacga
     4141 tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg cgtgcgatgg
     4201 cgtgacaacc gcgcggatgg cggaacaaag ccttgaggca ggtcattaca gcctggtggt
     4261 actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta tccggcagaa
     4321 aaaatatacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc
     4381 cgggctggat gtcggtgccg acgactatct ggtgaagcct tttgcgctgg aagagttaca
     4441 tgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg agctgattgt
     4501 tggcaatctg acgctgaaca tgggtcgccg tcaggtatgg atgggcggtg aagagttgat
     4561 tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag gcagtccggt
     4621 gcatcgggaa attctctaca acgacatcta taactgggac aatgaaccct cgaccaacac
     4681 cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta tccgcaccgt
     4741 gcgcggcttt ggctatatgc ttgtcgcgaa tgaggaaaac taattgaatc tgatgcgttt
     4801 tctgcgccga ccaatatcgc tgcgccaacg gctgatattg accatcgggg ccattttgtt
     4861 ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc agattcagct
     4921 gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc gtgagatccg
     4981 cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga cgctatttat
     5041 ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa aagagctgga
     5101 agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc tcgaaatcga
     5161 agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc tggataacga
     5221 aaggttattt accgctgacg tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt
     5281 gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag tggcacggct
     5341 tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg gacagtcatt
     5401 ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc cctcgtatga
     5461 cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag agagcgccgc
     5521 cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa acctggtaga
     5581 aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc aagaagatgg
     5641 cggagcggtc atggccgttg aagatgaagg accgggtatt gatgagagta aatgcgggga
     5701 gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc tggggttaag
     5761 tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa accggcaaga
     5821 gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg caaaccagat
     5881 ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt cataaacacg
     5941 gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca gagattaagt
     6001 gaaccttaag agttcaattg gcctgataag acagcgttac atcaggccat ccgtttcagc
     6061 ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct gcaaatcggc
     6121 aatctcgtgg tcaatatcat cgattttctg ctcgatatta tcgtaatgct cgacgagaat
     6181 ttccttcgct tcctgtatat ctgacgccgc cggtgttgca cctttcaacg gacgatttgc
     6241 cgtctctttc atggttacgc cggtgattaa accaaccacc gccactacca tcaggtaata
     6301 ggctggcatc atcagattct gcgagctttc gaccagccag gccgccagtg ttggcgtcag
     6361 accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt
     6421 cgggaacatc gctggcaagg tagaagccat aacgcccgta aagcagttaa ggatcaccgc
     6481 cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca gaataaacgc
     6541 cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac gacggccaaa
     6601 acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca taatggcgat
     6661 aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat aactcggcat
     6721 ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa
     6781 caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc catcctgcaa
     6841 accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg gagtttcttc
     6901 cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa agaacggaat
     6961 acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta acaccaccac
     7021 gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc ccataaagcc
     7081 acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat attcaccgcc
     7141 gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc aaataccaat
     7201 cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa tcacaatagt
     7261 gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga agagtccgcc
     7321 aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca tctgcacgct
     7381 ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac cataaacacc
     7441 aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt tacgcagttt
     7501 accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt tccttttcag
     7561 catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg
     7621 ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta gcgggtttgc
     7681 ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat tgggcagtta
     7741 atgacctttt taccctacca gcgtttaaat ttgtgatcaa cttcacacat ggatttcgtt
     7801 taggactcat tgatgtaact gattatttac accaaattta cagagaagat ttcctcgagc
     7861 attgcccccg ccagacgctg cgctgaacag agtcgtggca tccccagcgc gacagtttgc
     7921 cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca ctttcccagc
     7981 caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc aaaccactga
     8041 tggtaatagt gacgcgtggt cggggccgca ttgatactct ctgccagatg ttgtgtcgct
     8101 ttcacgcgca aatcacccag caacaacgcc accgcttgcg ggcttcccag acgacaggca
     8161 tcgccaaatg ccccccaacg caactcttcg agaacgataa aacagcgccc aggcaacgac
     8221 caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg ttcacccgcc
     8281 tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg ggctgaaaac
     8341 cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg ctggcggtga
     8401 tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagtgccag atgaccggga
     8461 tccagcatcg acgccagttt ttccaccaat gtcagacgca gactaagcgg cgcatttaac
     8521 atcccgccgt tcgtccatgt ctgaagctgc acgcgcgcaa gaagttcctg catcagtcgt
     8581 tcacgaaact gctgctgatg ggcttgtgga aggcgggcat catcgccctg cgccagatcc
     8641 actaaaaagc ggggataaac cgactccagc acgcgaccgg ggccgtccag taacgtcttg
     8701 gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc tgagcgtcgg
     8761 tgtaaatcca gccgtgagtc atcacagtct gctgcaattg ctgttgcatc agcctgacca
     8821 ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt aattccgtca
     8881 aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat tcatcgacct
     8941 gctctgccag aactttagcc atttgttggc aataaagatc gacttctgcg cttaatgctc
     9001 gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc cgtccccagt
     9061 caggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaatcgc gtgctctgct
     9121 cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg ttgaagtggg
     9181 gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac tcgctcaatt
     9241 ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg caaagggtgg
     9301 attcaagctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc gccgtctgta
     9361 acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc tgaaacaagg
     9421 cggctaactg caccttcagc gcatcttgct gttgacgcag aaagtggttg gcggaggtca
     9481 gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg agttgcaata
     9541 gctgcaaact ttcttcgatc tggtggatat tttgccgcaa ttgttcacaa gcgacgttta
     9601 acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc aacagtttat
     9661 gcgcggcaga tcgcagagca tacaacgagg cgttagcgta agcggcatga agcaacatct
     9721 gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga cgaatatgtt
     9781 cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg gcaaaatctt
     9841 ccacccagcg ttgttgctct ggcggtggta acttaccgtt gttggctaac tcatggcgcg
     9901 cccgatttgc caggtagccc cacatcgacg acaccggaaa tatctgctgt ggcgtaatac
     9961 agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg tcactgttac
    10021 gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc cccaccgcca
    10081 aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa tccagcaccg
    10141 ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga tgcggttgcc
    10201 cggcttcatt tggcccgggg gtatccagta acgtcaattg accgggataa ctttccagcc
    10261 ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg gcgtaagcag
    10321 aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac caaatcattc aaacttttca
    10381 gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg ccattttcga
    10441 tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc agatgcttaa
    10501 tatcgcaatc acgcaggcgc tgttgtaatt tttgaattaa acaatcgatt ggcgcgacat
    10561 gtgaaaaatg cagtaccggt tccttttggc cgggcgtatg gcgaataagc gtcggcagcg
    10621 cagtcattgg gcgattgcga ttaggcagta cctccgtacc aacaatggca ttaatggtgg
    10681 ttgatttccc tgctttcatg gtaccgacaa tcgccagcac catttccagt cgggaaattt
    10741 tacgcaactc attattcagc atcgcgtgac gctcggcgat attaggctga ctccagggta
    10801 aagccagttg tggcgcatcg tctccgggta cagagagagg cattttttcc agtaactgca
    10861 actgttggcg agaaagctgt aacagccgtt cagcctcctg acttaactca tacagggtct
    10921 gtgtgtacat a
//
LOCUS       DBFIFS010000151        10887 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.151, whole genome shotgun sequence.
ACCESSION   DBFIFS010000151 DBFIFS010000000
VERSION     DBFIFS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.151"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..270
                     /gene="ecpR"
                     /locus_tag="N7A07_003408"
                     /gene_synonym="matA"
     CDS             <1..270
                     /gene="ecpR"
                     /locus_tag="N7A07_003408"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU2478008.1"
                     /translation="NSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITDREME
                     IIRMTAQGMLPKSIARIENCSVKTVYTHRRNAEAKLYSKLYKLVQ"
     gene            345..932
                     /gene="ecpA"
                     /locus_tag="N7A07_003409"
                     /gene_synonym="matB"
     CDS             345..932
                     /gene="ecpA"
                     /locus_tag="N7A07_003409"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU2478009.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            990..1658
                     /gene="ecpB"
                     /locus_tag="N7A07_003410"
                     /gene_synonym="yagY"
     CDS             990..1658
                     /gene="ecpB"
                     /locus_tag="N7A07_003410"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU2478010.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1684..4209
                     /gene="ecpC"
                     /locus_tag="N7A07_003411"
                     /gene_synonym="yagX"
     CDS             1684..4209
                     /gene="ecpC"
                     /locus_tag="N7A07_003411"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU2478011.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4199..5842
                     /gene="ecpD"
                     /locus_tag="N7A07_003412"
     CDS             4199..5842
                     /gene="ecpD"
                     /locus_tag="N7A07_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU2478012.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5811..6521
                     /gene="ecpE"
                     /locus_tag="N7A07_003413"
     CDS             5811..6521
                     /gene="ecpE"
                     /locus_tag="N7A07_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU2478013.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6834..7163
                     /locus_tag="N7A07_003414"
     CDS             6834..7163
                     /locus_tag="N7A07_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU2478014.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7411..8025)
                     /locus_tag="N7A07_003415"
     CDS             complement(7411..8025)
                     /locus_tag="N7A07_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGU2478015.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8443..9132
                     /gene="paoA"
                     /locus_tag="N7A07_003416"
     CDS             8443..9132
                     /gene="paoA"
                     /locus_tag="N7A07_003416"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU2478016.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9129..10085
                     /gene="paoB"
                     /locus_tag="N7A07_003417"
     CDS             9129..10085
                     /gene="paoB"
                     /locus_tag="N7A07_003417"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU2478017.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10082..>10887
                     /gene="paoC"
                     /locus_tag="N7A07_003418"
     CDS             10082..>10887
                     /gene="paoC"
                     /locus_tag="N7A07_003418"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU2478018.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKV"
BASE COUNT         2766 a         2903 c         2840 g         2378 t
ORIGIN      
        1 aacagcaata ttaggggcgt ggtatacgct ggactgagtc gtgatattag aaaagaactg
       61 gcctatgtga ttaatggcag gttcctgaga aaagatatta agaaagataa aatcactgac
      121 cgggaaatgg aaattatccg catgacggct cagggaatgc tgcctaaatc gattgccaga
      181 attgaaaatt gtagtgtaaa gacagtgtat acccatcggc gtaatgcaga ggccaagctg
      241 tactcaaaat tatataagtt ggttcagtaa actccaggca agttagtttt aaaaaatgac
      301 tcactgggac atcacgtcct caattcaact cgggaagaaa tacaatgaaa aaaaaggttc
      361 tggcaatagc tctggtaacg gtgtttaccg gcatgggtgt ggcgcaggct gctgacgtaa
      421 cagctcaggc tgtagcgacc tggtcggcaa cagccaaaaa agacaccacc agtaagctgg
      481 ttgtgacgcc actcggtagc ctggcgttcc agtatgccga aggcattaaa ggttttaact
      541 cacagaaagg tctatttgac gtggctatcg agggtgactc aacggctacc gcctttaaac
      601 tgacctcacg tcttatcacc aacacattaa cccagttgga tacctcaggt tccacactga
      661 atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac tggcgatacc gtgatgatcg
      721 ataccgccaa cggcgtactg ggcggcaacc ttagcccgct ggcaaacggt tacaatgcca
      781 gcaatcgtac caccgcacag gatggtttca ctttctccat catcagcggc accaccaatg
      841 gtaccaccgc agtaaccgat tacagcactc taccggaagg catctggagc ggcgacgtta
      901 gcgtacagtt cgacgcgacc tggaccagtt aatctctctg atgtaccagc aggggtagcc
      961 ccccagcttt attcctggac ggaattgtta tgaaaaagca ccttctgctt ctcgctctgc
     1021 tgttatccgg aatatctccg gcccaggcgc tggatgtcgg cgatatatca tcgtttatga
     1081 acagtgacag cagcacgctg agcaaaacga tcaaaaacag taccgacagt ggtcgcctta
     1141 tcaatatccg tctcgaacgg ctctcttcac cgcttgacga cgggcaggtt atctcaatgg
     1201 acaagccgga cgagttgcta ctcactcccg ccagcttgct gctacccgcc caagccagcg
     1261 aagtgatccg cttcttctat aagggaccag cagatgaaaa agagcgctac taccgcattg
     1321 tctggtttga tcaggccctc agtgatgcgc agcgcgataa tgccaaccgc agcgctgtgg
     1381 ccactgcttc cgcccgcatc ggcaccattc tggtcgtcgc cccccgccag gcaaactacc
     1441 actttcagta cgccaacggc accctgacaa atacaggaaa tgcgacgctg cggatcctcg
     1501 cctacggccc ctgcctgaaa gccgccaatg gtaaagagtg taaagagaat tactacctga
     1561 tgccgggcaa gtcgcgtcgt tttacccgcg tggacacggc ggataacaaa ggacgggttg
     1621 cactttggca gggtgataag ttcattcccg tgaaatagat agctgtgcag atggataacg
     1681 acaatgcctt tacgacggtt ctccccagga ctgaaagccc agtttgcctt cggcatggtc
     1741 tttttgttcg ttcagcccga tgccagcgct gctgacataa gtgcgcagca aataggtggg
     1801 gtgattattc cgcaggcctt cagtcaggcg cttcaggacg gcatgagcgt cccgctctat
     1861 attcatctcg ccggtagcca gggtcgccag gacgatcagc gaatcggcag cgcttttatc
     1921 tggttggatg atggacagct acgcatccgg aaaatacagc tggaagagag tgaagataac
     1981 gccagtgtca gcgaacaaac tcgacagcag ctgatggctc tggcgaacgc cccgttcaat
     2041 gaggccctta ccatccccct gactgacaac gcgcagctgg atctcagctt gcgccaactg
     2101 ctgctgcagc tggtggtcaa gcgcgaagcg ctgggcactg tactacgctc acgtagcgaa
     2161 gacatcgggc agtccagtgt taacaccctc agcagtaatc tgagctataa cttcggcatc
     2221 tataacaacc agttgcgtaa cggcgggagc aacacatcca gctatctgtc gctgaataac
     2281 gttactgcac tgcgcgaaca tcatgtggtg ctcgacggct cgctgtacgg gatcggtagc
     2341 ggtcaacagg acagtgaatt atataaagcg atgtatgaac gcgattttgc cggtcaccga
     2401 tttgccggtg gaacgctcga cacctggaac ttgcagtcct tagggccgat gaccgccatt
     2461 tcagcaggga agatttacgg cctttcctgg ggaaaccagg ccagctccac catcttcgac
     2521 agcagccagt cagccacgcc agtgatcgcc tttttaccgg cggcgggtga agtacatctc
     2581 acccgtgatg ggcggttact aagcgttcag aacttcacca tgggcaatca tgaagtggat
     2641 acccggggtc taccatacgg tatttacgat gtggaagttg aggtgatcgt taacggtcgc
     2701 gtgatcagca aacgcaccca gcgggtcaat aagctgttta gccgggggcg cggcgtcggt
     2761 gcaccactgg cgtggcaggt atggggcggt agctttcata tggatcgctg gtcggaaaac
     2821 gggaaaaaga cgcgaccagc taaagagagt tggctggcag gtgcctcgac ctccggctca
     2881 ctgagtacgc ttagctgggc ggcaacggga tatggatacg ataatcaggc ggtgggtgaa
     2941 acccgtctga cgctgccgct tgggggagcg atcaacgtta acctgcaaaa tatgctggcc
     3001 agtgacagct catggagcag catcggcagc atcagcgcca ctctaccggg aggctttagt
     3061 tcgctgtggg ttaatcagga aaaaacccgc attggcaatc aattgcgacg tagcgatgcc
     3121 gacaaccgtg ctatcggcgg cacactcaac ctgaactcac tgtggtcgaa gctgggcaca
     3181 ttcagcatca gctacaatga tgaccgccgt tacaacagcc attattacac ggcagattac
     3241 tatcaaaatg tctacagcgg tacctttggt tcgcttggcc tgcgggccgg tattcagcgc
     3301 tataacaacg gcgacagcaa cgccaataca gggaaatata tcgctctcga tctctcgcta
     3361 ccactgggca actggtttag cgcagggatg actcatcaaa acggctacac catggcaaac
     3421 ctgtcagcac gcaagcagtt tgatgaaggg accattcgca ctgttggtgc caatctgtca
     3481 cgagccatct ccggcgatac cggtgatgac aaaactctca gtggtggggc gtatgcacag
     3541 ttcgacgctc gctacgccag cggaacgctg aacgtcaata gcgcggcgga cggctacgtc
     3601 aataccaatt taaccgccaa tggcagcgtc ggctggcagg gtaaaaacat tgctgccagc
     3661 gggcggactg atggcaacgc tggggtgata ttcaacaccg ggctggagga cgacggtcag
     3721 atcagcgcca aaatcaacgg gcggattttc ccgcttaacg gcaagcgtaa ctatctcccg
     3781 ctctctccct atggaagata tgaggtggag ttacagaaca gcaaaaactc actcgacagt
     3841 tacgatatcg tcagcggtcg caaaagtcat ctgactctct atccaggcaa tgtcgctgtc
     3901 attgagccag aggtgaagca gatggttacc gtctccggtc gtatccgtgc ggaagacggc
     3961 acactgctgg ctaacgcacg gattaacaac catatcggcc gaacccgaac cgatgaaaac
     4021 ggcgagtttg tcatggacgt ggataagaaa taccccacta tcgattttcg ctacagtggc
     4081 aataaaacct gcgaagtggc actggaactc aaccaggcgc gcggtgccgt ctgggtcggt
     4141 gatgtggtct gcagcggcct ctcatcgtgg gcggcggtga cgcagacagg agaagagaat
     4201 gagagttaac ctactgatag cgatgataat ctttgcgcta atctggccag taactgcgct
     4261 cagagcggca gtgagcaaaa caacctgggc ggatgcaccg gcacgcgagt ttgtgtttgt
     4321 cgaaaacaac tcagacgaca actttttcgt cactcctggc ggggcgctgg atccgcgcct
     4381 gaccggtgcc aaccgctgga ccggtttaaa atacaatggt tcaggaacca tctatcagca
     4441 aagcctcggc tacattgata acggttacaa caccggcctt tataccaact ggaagtttga
     4501 tatgtggctg gaaaattcac cagtttcatc tcctttaact ggcttgcgct gcatcaactg
     4561 gtacgctggg tgtaatatga ccaccagtct tatcctgccg caaaccaccg acaccagtgg
     4621 attttatggc gcgacggtga ccagcggcgg cgcgaagtgg atgcacggca tgttgtcaga
     4681 cgcgttttac cagtatctgc aacaaatgcc cgtcggcagc agctttacaa tgaccatcaa
     4741 tgcctgccag acctctgtga actatgacgc cagcagcggc gcacgctgta aggatcaggc
     4801 ctccggcaac tggtatgttc gcaacgtcac ccatacgaaa gcagcaaatc tacggttgat
     4861 aaatacccac tcgctggcgg aagtatttat caacagcgac ggagtaccga ctctgggcga
     4921 agggaacgcc gactgccgga cgcaaaccat cggcagccgt tcaggattaa gttgtaagat
     4981 ggttaactat accctgcaaa caaacggact cagcaacacc tcaatccata tattcccggc
     5041 gatcgccaac tcgtcgttag cctcggccgt cggggcgtac gatatgcagt tcagtctgaa
     5101 tggcagttca tggaaaccgg tgagcaatac tgcctattac tacaccttca acgagatgaa
     5161 gagcgcagac tcgatctatg ttttcttctc gagcaacttc tttaagcaga tggtgaacca
     5221 gggaatcagc gatatcaaca ccaaagatct attcaacttt cgctttcaga acaccacatc
     5281 accggagtct ggctggtatg aattttctac ctccaacacg ctgattatca aaccccgtga
     5341 tttcagcatc agtattatct ccgatgaata tactcagaca ccgtcgcggg agggatatgt
     5401 cggcagcggc gagtcggcac tcgatttcgg ctatatcgta accaccagcg gtaaaacagc
     5461 tgccgacgaa gtgctgatca aggtgaccgg ccccgcgcag gtgattggcg ggcgctccta
     5521 ttgtgtcttc agctccgatg acggtaaggc gaaagtaccg ttcccggcga cgctttcctt
     5581 tattacccgc aacggagcta caaaaaccta cgatgccggg tgcgatgata gctggcggga
     5641 tatgaccgat gcgctgtggt tgaccacacc gtggactgat atctctggcg aagtggggca
     5701 gatggataag accacagtca aattttcgat tccaatggat aacgccattt ctctgcgtac
     5761 ggtagatgat aacggctggt ttggcgaagt cagcgcttca ggagaaattc atgttcaggc
     5821 gacgtggcgt aacattaact aaggccctgc tgacagcggt ctgtatgctg gcggcacctt
     5881 tgacacaggc gatttcggtc ggcaatctga ctttttcact gccgtccgag actgactttg
     5941 tcagcaaacg tgtagtgaat aacaacaaaa gcgcgcggat ataccgtatt gccatcagtg
     6001 ctatcgatag cccgggcagc agtgaattac gcacccgacc ggtggatggt gaactgcttt
     6061 tcgccccccg ccagctggcg ttgcaggctg gtgagagcga gtattttaaa ttttactatc
     6121 atgggccaca ggataaccgc gagcgctact accgggtctc atttcgcgag gtccccactc
     6181 gtaacctgac aaaacgcagc cctaccggcg gtgaggttag cacggagccg gtggtggtga
     6241 tggataccat tctggtagta cgaccacgtc aggttcagtt taaatggtcc ttcgatcagg
     6301 tgaccggaac ggtaagtaac accggcaaca cgtggtttaa gctactgatt aaaccaggat
     6361 gtgattcgac cgaagaggaa ggcgatgcct ggtatctacg tccggaagac gtggttcatc
     6421 agcctgagtt acgtcagccg gggaatcact atctggtcta taacgacaaa ttcattaaga
     6481 ttagcgattc ctgtccggct aagccccctt cggcggactg atacttatcc atcggtcgag
     6541 ggaagaattc cacctcagag actcaaaaat cgtttaaatg atgtggaagc gatcgtcaat
     6601 atcacctgta acgataggct ttctcatatg cacaccacag tgttcaggag acacggaaag
     6661 gtaccaggcg gcctgctaac gagtggtttc ttcactaatt aggacgtaaa atcggtaacg
     6721 gctggaaatc attcaatact cacactatcg aaagtacacc agccaaccgc gatacgtccc
     6781 tgcatacgac gtaccgcagt tctctttacg atttatagct gtactggtga attatgagca
     6841 atctgaatcc atgcatgacg tgtggtgcct gttgtgcatt tttccgcgtc tctttctact
     6901 gggccgaagc cgacgatgct ggcggcacta ttcccgccag gctcactgaa caaatatccc
     6961 cttttcaccg atgcatgagc ggaactaatc agaaaaaccc ccgatgtatt gcccttgcag
     7021 gaaccccggg caaaaatgcc tgctgcacga tatataaaaa tcgatcgtcc acatgcagag
     7081 aattcgccat gtctggtgag aacggagtcg tcaatgaggc ttgcaatcgt gcaagggcta
     7141 aatacgggct gacaccgtta taaatataca ataattattt gcactgcccc gccagctgta
     7201 atagcggggc ttcgtttttc aggggtaaca aaccccgata cttcttctat ttgccagcaa
     7261 caatgcctct cttctgtagc gttcttcgga tctacatcat cctgaagtag cgcgatatac
     7321 tcactgacta tctgaatcag aatataaaaa gcaatgtttt taacctataa aaatggcgct
     7381 gtatttgcgc catttttatc attcaatgca ttatctgttt gagcctaaag ggatctcagg
     7441 gtctggctca tgagtaattc tgtttcgtaa atctctgcga ataatttcaa tggaccagaa
     7501 ccagactaaa tgtccaaaaa tttcagaaac attctcatac cacgggagat caaacagagg
     7561 tggtgtcagt cccatgagag ggaatgaaat catatgaaca aaaagttggg ctaaagcacc
     7621 tgccagtaag ccctgccaga gtttaatttt tggaaatact tctgcgacca cacaataacc
     7681 gacagcaaac actatcgaaa agataatgtg cgtaacacca acccagttaa agacatgccc
     7741 ggcaaaggta taaacagccg cattgggatc tgtcagcccc agccagtctc gaagaaaaat
     7801 atacggagga ttgagaaaat tacgcgagca atcaatttgc ccagcagccc tgattaatga
     7861 ttccgggcca cacgctgcat taaacatatc caccgggcta cgtggcggca atggaacttc
     7921 agccccccac ttcacaaatg cggaaacaac gccagcaatc agcccaatga atgcagcaag
     7981 accataacgt ctgcggttcg gtggagtttg ttcaaatata ttcatatcta ccctgcttgt
     8041 accattatgt tatacacctc tttaggagta ttcataaaac aaggtaaatg taaagaactg
     8101 tattgttttg tataacaaga tggttgtcta atcaccaaag aatataagca ccatcatttc
     8161 tccctatttt tatattagaa gtgacataga tttgcagggt gatgcagagc tgacaatcac
     8221 aggtttcctt atcggttttt gcatcgtaca actaaagcaa taaaccagct cccatcatca
     8281 tcaacatccc ggcgaacatg aatttattag ccgggctcat agctgcattc gcgttttaag
     8341 aataatcctc ctgctgtcgc cgactatgct taacgtttaa aaaagcatca gcactctcgc
     8401 aacgcactct tattttcccc tttagaatac cggaggcctg gtatgagcaa ccaaggcgaa
     8461 taccccgaag ataatcgggt tgggaagcat gagccgcacg atttaagttt gacccgtcgc
     8521 gatctgatta aagtgagcgc cgcaacggcg gcggccgccg tggtttatcc tcattctacg
     8581 cttgcggcaa gcgttccggc agctacaccc gcgcctgaga taatgcccct gacacttaag
     8641 gtgaacggca aaaccgagca gcttgaggtg gatacccgaa ccacgctact ggacgctttg
     8701 cgtgaaaatc tgcatttgat cggtaccaag aaaggttgcg atcacggaca gtgcggagcc
     8761 tgtaccgtgc tggtcaatgg tcgcaggctt aatgcctgcc tgacgcttgc agtcatgcat
     8821 cagggggccg agatcaccac cattgaaggc ctgggttcac cagataatct tcaccccatg
     8881 caggcggcct ttatcaagca tgatggcttc cagtgcggct actgcacctc cgggcaaatt
     8941 tgctcatcag tagcggtgct aaaagagatt caggacggca ttcccagtca cgtcacggtc
     9001 gatttggttt cccctccaga aagaactgcc gatgagatcc gtgaacgtat gagcggcaac
     9061 atctgtcgct gtggtgcata cgctaacatc cttgccgcca ttgaagacgc tgcgggggag
     9121 ataaaatcat gaaggcgttt acctatgaac gagtgaacac ccccgccgag gcggcactta
     9181 gcgctcagcg cgtacccggc gcaaaattta tcgcgggcgg gaccaatctg ctggacctga
     9241 tgaagctgga aattgaaacg cccacccacc ttatcgatgt gaacgggctc gggctcgata
     9301 agatcgaagt gaccgacgcg ggtgggctgc gcatcggcgc actggtacgg aacaccgacc
     9361 tggtggctca cgagcgcgtg cgtcgtgatt acgcggtact ctcccgcgcc ctgctcgctg
     9421 gcgcgtctgg tcagttacgc aatcaggcaa ccacagcagg taatctgctc cagcgcacgc
     9481 gctgccccta tttttacgac accaatcagc cctgcaataa gcgcctgccc gggagcggct
     9541 gcgcggcgct tgaaggcttt agccgtcagc acgcggtggt aggcgtaagc gaagcctgca
     9601 ttgccaccca tccgagcgat atggcggtcg caatgcggtt gctggatgcg gtggtggaaa
     9661 ccatcacgcc ggagggaaag actcgcagta tcacactggc tgatttttat cacccaccgg
     9721 ggaaaacgcc gcacattgaa accgccctgc ttcccggtga gcttatcgtt gcggtgacgt
     9781 tacctccgcc gctcggcgga aaacatatct accgtaaggt gcgcgatcgc gcctcctacg
     9841 cctttgccct ggtatcggtc gcggcgatta ttcagcctga cggcagcggg cgcgtcgcgc
     9901 tgggcggagt agcacataag ccctggcgca ttgaggctgc ggatgctcag ctatcccagg
     9961 gggcgcaggc cgtatatgac gcgctgttcg ccagcgccca tcccaccgct gaaaacacct
    10021 ttaaactcct gttggcgaag cgaacgcttg cctccgtact ggctgaagcg agggcacaag
    10081 catgaaattt gataaacccg caggggaaaa cccgatcgat cagctgaagg ttgtcggtcg
    10141 tccccatgac cgcatcgacg gaccgctgaa aactaccggc acggcacgct acgcctacga
    10201 atggcatgaa gaatccccca acgccgccta tggctatatc gtcggttccg ccattgccaa
    10261 aggacgcctc accgcccttg atacggacgc cgcgcaaaaa gcgccgggcg tactgcctgt
    10321 cattaccgcc agtaacgccg gggcactcag taaaggcgac aaaaacaccg ccaggctgtt
    10381 aggcggcccc actattgagc actatcatca ggccattgct ctggtagtgg ccgagacctt
    10441 cgaacaggcg cgagcggcgg cctcgctggt gcaggcgcac tatcgccgta ataaaggagc
    10501 ttactccctg gcggacgaaa aacaggccgt cagtcagccg ccggaagaca cgcccgacaa
    10561 gaacgtcggt gactttgacg gggctttctc ctccgctgcg gtgaagattg atgctaccta
    10621 cacgaccccg gaccagagcc atatggcgat ggagccgcat gcctcgatgg ccgtctggga
    10681 tggaaataag cttactctct ggacctcaaa tcagatgatt gactggtgcc gcaccgatct
    10741 ggcaaaaacg ctgaaagtgc ccgtggagaa tgtgcgtatt atctccccgt atatcggcgg
    10801 agggtttggc ggcaagctgt tcctgagaag cgatgcgctg ctggcggccc tcgccgcccg
    10861 agcggtgaaa cgtccggtta aagtgat
//
LOCUS       DBFIFS010000152        10883 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.152, whole genome shotgun sequence.
ACCESSION   DBFIFS010000152 DBFIFS010000000
VERSION     DBFIFS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.152"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            262..1602
                     /gene="dcuB"
                     /locus_tag="N7A07_003419"
     CDS             262..1602
                     /gene="dcuB"
                     /locus_tag="N7A07_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU2478019.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1680..3326
                     /gene="fumB"
                     /locus_tag="N7A07_003420"
     CDS             1680..3326
                     /gene="fumB"
                     /locus_tag="N7A07_003420"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2478020.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            3449..4078
                     /gene="yjdF"
                     /locus_tag="N7A07_003421"
     CDS             3449..4078
                     /gene="yjdF"
                     /locus_tag="N7A07_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU2478021.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4217..5638)
                     /gene="melB"
                     /locus_tag="N7A07_003422"
     CDS             complement(4217..5638)
                     /gene="melB"
                     /locus_tag="N7A07_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU2478022.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(5741..7096)
                     /gene="melA"
                     /locus_tag="N7A07_003423"
     CDS             complement(5741..7096)
                     /gene="melA"
                     /locus_tag="N7A07_003423"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU2478023.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7379..8287
                     /gene="melR"
                     /locus_tag="N7A07_003424"
     CDS             7379..8287
                     /gene="melR"
                     /locus_tag="N7A07_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU2478024.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8486..10753
                     /gene="adiA"
                     /locus_tag="N7A07_003425"
     CDS             8486..10753
                     /gene="adiA"
                     /locus_tag="N7A07_003425"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU2478025.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQVMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2776 a         2893 c         2671 g         2543 t
ORIGIN      
        1 cagcaaacaa aacattaaca tctgcgcagt acaaacttta aacccatcgc cagagagttt
       61 ttctctctga aaaagccgct tatcacagtg cataaatttg ccgctgcttt aatcagccaa
      121 tattcactgt gaggtatttg ctaaagccgg taacgaccaa acggatattt agtcaggctc
      181 tgaaaacagt tcatacaaaa cagaacgtga ctgtgatcta ttcagcaaaa atttaaatag
      241 gattatcgcg agggttcaca catgttattt actatccaac ttatcataat actgatatgt
      301 ctgttttatg gtgccagaaa gggtggtatc gcgctgggtt tattaggcgg tatcggtctg
      361 gtcattctgg tcttcgtctt ccaccttcag ccaggtaaac caccagttga tgtcatgctg
      421 gttatcattg cggtggtggc ggcatcggcg accttgcaag cttcgggcgg tcttgatgtc
      481 atgctgcaaa ttgccgagaa gctgctgcgc cgcaacccga aatatgtctc aattgtcgcg
      541 ccgtttgtga cctgtacgct gaccattctt tgcggtacgg gtcatgtggt ttacaccatt
      601 ctgccgatca tctacgacgt cgccattaag aacaacatcc gtccggaacg tccgatggcg
      661 gcaagttcta tcggtgcaca gatggggatt atcgccagtc cggtgtcggt tgcggtcgtg
      721 tctctggttg caatgctggg taatgtcacc tttgatggtc gccatcttga gttcctcgac
      781 ctgctggcaa tcaccattcc atcgacgtta atcggtatcc tggcgatcgg tatcttcagc
      841 tggttccgcg gtaaagatct ggataaagac gaagagttcc agaaattcat ctccgtaccg
      901 gaaaaccgtg agtatgttta cggtgatacc gcgacgctgc tggataaaaa actgccgaaa
      961 agcaactggc tggcaatgtg gattttcctc ggggcaatcg ctgtagtcgc acttcttggt
     1021 gctgattcgg acctgcgtcc atccttcggc ggcaaaccgc tgtcgatggt actggttatt
     1081 cagatgttta tgctgctgac cggggcgctg attattatcc tgaccaaaac caatcccgcg
     1141 tctatctcaa aaaacgaagt cttccgttcc ggtatgatcg ccatcgtggc ggtgtacggt
     1201 atcgcatgga tggcagaaac catgttcggt gcgcatatgt ctgaaattca gggcgtactg
     1261 ggtgaaatgg tgaaagagta tccgtgggcc tatgccattg ttctgctgct ggtttccaag
     1321 tttgtaaact ctcaggctgc ggcgctggcg gcgattgttc cggtcgcgct agcgatcggc
     1381 gttgatccgg catacatcgt ggcttcagca ccggcttgct acggttatta catcctgccg
     1441 acttatccga gcgatctggc agcgattcag tttgaccgtt ccggcaccac ccacatcggt
     1501 cgcttcgtca tcaaccacag ctttattctg ccggggttga ttggtgtgag cgtatcgtgc
     1561 gtcttcggct ggatcttcgc cgcgatgtac gggttcttat aaatgcactt tgcgtgccgc
     1621 ccgttactac gcggcacgcc attttcgaat aacaaataca gagttacagg ctggaagcta
     1681 tgtcaaacaa accctttatc taccaggcac ctttcccgat ggggaaagac aataccgaat
     1741 actatctact cacttccgat tacgttagcg ttgccgactt cgacggcgaa accatcctga
     1801 aagtggaacc agaagccctg accctgctgg cgcagcaagc cttccacgac gcttctttca
     1861 tgctccgtcc ggcacaccag aaacaggttg cggctattct tcacgatcca gaagccagcg
     1921 aaaacgacaa gtacgtggca ctgcaattct taagaaactc cgaaatcgcc gccaaaggcg
     1981 tgctgccgac ctgccaggat accggcaccg cgatcatcgt cggtaaaaaa ggccagcgcg
     2041 tgtggaccgg tggcggtgat gaagaagcgc tgtcgaaagg cgtctataac acctatatcg
     2101 aagataacct gcgctactcg caaaacgcgg cgctggacat gtacaaagag gtcaacaccg
     2161 gcactaacct gcctgcgcaa atcgacctgt acgcggtaga tggcgatgag tacaaattcc
     2221 tttgcgttgc gaaaggcggc ggctctgcca acaaaaccta tctctaccag gaaaccaaag
     2281 ccctgctgac tcccggcaaa ctgaaaaact tcctcgtcga gaaaatgcgt accctcggta
     2341 ctgcagcctg cccgccgtac catatcgcgt ttgtgattgg cggtacgtct gtggaaacca
     2401 acctgaaaac cgttaagtta gcaagcgctc actattacga tgaactgccg acggaaggga
     2461 acgaacatgg tcaggcgttc cgcgatgtcc agctggaaca ggaactgctg gaagaggccc
     2521 agaaactcgg tcttggcgcg cagtttggcg gtaaatactt cgcgcacgac attcgcgtta
     2581 tccgtctgcc acgtcacggc gcatcctgcc cggtaggcat gggcgtctcc tgctccgctg
     2641 accgtaacat taaagcgaaa atcaaccgcg aaggtatctg gatcgaaaaa ctggaacacc
     2701 atccaggtca gtacattcca caagaactgc gccaggccgg tgaaggcgaa gcggtgaagg
     2761 tcgaccttaa ccgcccgatg aaagagatcc tcgcccagct ttcgcaatat ccggtttcca
     2821 ctcgtttgtc gctcaccggc accattatcg tgggccgaga tattgcacac gccaagctga
     2881 aagagctgat tgacgccggt aaagaacttc cgcagtacat caaagatcac ccgatctact
     2941 acgcgggtcc ggcgaaaacc cctgccggtt atccatcagg ctcacttggc ccaaccaccg
     3001 caggtcgtat ggactcctat gtggatctgc tgcaatccca cggcggcagc atgatcatgc
     3061 tagcgaaagg taaccgcagt cagcaggtta ccgacgcgtg tcataaacac ggcggcttct
     3121 acctcggtag catcggcggt ccggcggcgg tactggcgca gcagagcatc aagcatctgg
     3181 aatgcgtcgc ttatccggag ctgggtatgg aagctatctg gaaaatcgaa gtagaagatt
     3241 tcccggcgtt tatcctggtc gatgacaaag gtaacgactt cttccagcaa atcatcaaca
     3301 aacagtgcgc gaactgcact aagtaacctc ttcggcccag cgcctggcag catgctgcca
     3361 ggtgatcccc ctgggccacc tcttttgcga ttgtaatttc acgcttgctg gtgaatagtc
     3421 agtattttcc ctgacttgcg aactcaccat gacccgcaca ctcaagccgt taattcttaa
     3481 caccggcgca ctggcgctaa cgttaatcct gatttatacc ggcatttcgg cccatgacaa
     3541 actcacctgg ctgatggaag tgacaccggt gattattgtc gtgccgctac tgcttgccac
     3601 cgctaaacgt tatccgttaa cgccgctgct ctatacgctc attttctttc acgccatcat
     3661 cctgatggtc ggcgggcaat acacctacgc gaaagtcccc atcggttttg aggtgcagga
     3721 atggctgggg ttgagccgta atccgtatga caagctgggg cattttttcc aggggctggt
     3781 gcctgcactg gtggcacgag agatccttgt gcgcgggatg tacgttcgcg gacgtaaaat
     3841 ggtggcgttt ctggtctgtt gcgtggcgct ggcgataagc gcgatgtatg aattaatcga
     3901 gtggtgggcg gcgctggcaa tgggccaggg agcggatgat tttctcggca cccagggcga
     3961 ccagtgggac acgcaatccg atatgttctg cgcgctgctt ggcgcattaa ccacggtgat
     4021 attcctcgct cgctttcatt gccgtcagct acggcgcttt ggcttgataa ccggataaca
     4081 acgtgcactt tgttgatgcc ggatgcggcg tgaattcctt atccggtcta caaaatttgc
     4141 aaatccaaag cattccatta agacgtggcc tgataaacat atcgcatcag gccattttcg
     4201 ttagctattg tccacgtcag gctttcacat cactcactgc accgaccgga atatcagcat
     4261 gaacaggctc gggcggtatt ttgcgatatt tatccagcag atgaatctgg atcctgcgca
     4321 gcgtgtcgcc attgaggcga tagaaacgga agtagagaat cagcgttacc atgaaaaaca
     4381 gagtcggtag agcaatcata ataaactgca tacctaatag ggcttgcgta gactgttcaa
     4441 cattcggtac atagccaatc atccctaaca caaccgcaat gaaaaaagcc gcaaaggctg
     4501 aaccgccctt caccaccata gtctgcacgg agtaagcgat gctttcacag cgtacgtgca
     4561 gtttatattc accgtaatca acggtatctg ccaccatgat gacctgtaat acccagaaaa
     4621 gcgccgttcc cacattcagc aaaatacccg caatcacaat gaggacgacg ttgtgatagc
     4681 tcattaatgc cattaacagg agaacaccac agcttaacac cggaagaata gatgctccgg
     4741 cccataaaat gcgtcgggat aatgatttaa ctaagcgtgg gaagaatact aacgtcacca
     4801 ggttagcagc tcccgcatac gacagataat aggggaacaa atccgcatca ccgataacat
     4861 atgagaaata atagatagca aagccggtaa taatgttgct ggctacatta taagcaagag
     4921 ccatacccaa gaggcatgaa agctgatcgt ttttataaat aagcgcaacg atggctttaa
     4981 gtgtcagatg gcttccttca gcagacggtt gattgtctga cgaaaagact tcatgcacat
     5041 tgcgcagagt gatgatggtt gaaacaataa aaaaggcgat cagtaccagt gtgaacatct
     5101 gaaagccaaa tccccgatcg ccaccgccga cataattaac aaatggtagc gtcacacctg
     5161 ccgtaacaaa gcccgccaga ctggcaaaaa aacgcggata aggaaccagt tgttcacgct
     5221 cacgtttatc gagcgtgatg gttggaacca gcgaccagaa gggaatatcc ataatggtgt
     5281 aagtcatgcc ccagaggatg taggtcacgc aaacaaaggc aatctgagta gtaccttcaa
     5341 acagatgcgc actaaagagg agaaataaga ttacagagtt tgccaacgta ccgatcagga
     5401 tccagggttt gaacttaccc catcgcgatc gcgtagcatt tacaatccat cccataatcg
     5461 gatcgttaat agcatcccag atcctcgcca ccagaaacaa agtaccgacc aaacccacag
     5521 acagcccgac gacatcggtg tagtaataca tgaggtacat atacacaatg ccgatcgcaa
     5581 aatccttccc gaacgctcca aatccataac tgagttttgt agtcattgaa atgctcatag
     5641 ggtatcgggt cgctgttacc aacgcccgcc ttctgttatt ggtatcgtca gaggtgtgcg
     5701 ccggaataaa tcccccggcg cagtagcgtt tagtcgcgtt ttaacggtgc aaccagcctg
     5761 gcagccagtc gccgtgggcg gcaatcaggt cgtcaacaag agcatatatt tcgtcaatgc
     5821 ccagcacggc ggcagtatgc gggtccatca tcgcagcgtg gtaaacacgg tcgcgatttt
     5881 ccgtaagaat agcttcggtc agcagcgtct gtacgttgat gttggtttgc atcagggcgg
     5941 ccagatgcga aggtagcgta ccgactttgg tcggctgaat gccattagca tcaaccagac
     6001 aggccacttc cacgcaacat ccttgtggca ggttatcaat caaaccatcg ttacggacgt
     6061 tgccgtaaat cacactcggc tcgcccgtcc agatagcgtt catgattgtg ctggcatatt
     6121 cccgtgacgg tttaatatca atccgggagg cgtttttata ctcctccagc tctttatgcc
     6181 agttcgccag ctgctcgacg cagcgtttcg ggtactcatc cagcggtact ttataacgct
     6241 caatcaaatc ctcacgacct ggcttaataa accacggtgt gtactcagca aaatgttctg
     6301 acgattccgt gacgaaatag cccagctttt tgaacatttc atagcgcaca atattctggc
     6361 agcgagtatt gccgtgaata ttcggcttcg gtgcctgccc tgcgtcataa gccgccagca
     6421 gttccgggta gagattcacg taactgccgt cggcggtttt gcgctccagc tccaggtaaa
     6481 acgccatatg gttgatacct gcgcaacggt aacgcagcgt agccgggtcg atattgaggt
     6541 cacgcgccag ctcttccgcc gttccctgca ccgaatggca cagcccgacc tgtttgatat
     6601 gcggatagcg ggcatacatc gcccaggtat tcatcgccat tgggttaaca tagttgagca
     6661 tggtggcatc ggggcagact tccgtcatgt cctcgcaaat ttgccacaga tgcggaatgg
     6721 tacgtagcgc gcgcataata ccgcccggcc ccaacgtatc ggcaatggtt tgttccagac
     6781 catgccgctt acagacctcg aaatcagtca ccgtgcaagg tgcataaccg ccaatctgaa
     6841 atgccaccac gacaaaatcg gcatcctgta aggcttcttt ctgttgggtg tggcaggtga
     6901 ttttgccgct ggcccctgct gaatccatca gcttacgcac cacaatatgc gactcttcca
     6961 ggcgggtggg gtcaatgtcc atcagggcaa tatgcgccgt tttcagcgcc tcgcgatgga
     7021 acacatcacc aagaatattt ttaacgaaaa tcgtcgaacc agcgccgata aatgtaattt
     7081 tgggtgcaga catcatgcag atctcctggc ttgcttgaat aacttcatca tggcaggcga
     7141 atctgcagaa aacctccggc ttccaggttg agcattccca taaactcaga tcctgcgtga
     7201 agcagcagta aatctgagtt tatgggaata atttacctga aaagcagagt tttcggcctc
     7261 atgatgctcg gacactcccc tcgcccctct gggaagaggg tgaaaaccgt gctcccactc
     7321 gcagtcatcc tccctcactc ctgccataat tctgatattc caggaaagag agccatccat
     7381 gaatacagat acgtttatgt gcagcagcga cgaaaaacag acccgcagcc cgctgtcgct
     7441 gtattcggaa taccagcgga tggagattga gtttcgcgca ccgcatatca tgcccaccag
     7501 ccactggcat ggtcaggtcg aagtgaatgt gcctttcgat ggcgatgtgg aatacctgat
     7561 caacaatgaa aaagtgaata tcaatcaggg acatatcacg ctgttctggg cctgtatacc
     7621 gcaccaacta acagataccg gaacctgtca gagcatggcg atttttaatc tgccgatgca
     7681 tctgtttctc tcctggccgc tggataaaga cctgattaac cacgtcactc acggcatggt
     7741 gatcaaatca cttgctacac agcaacttag cccgtttgaa gtgcgccgct ggcagcagga
     7801 attaaacagt ccgaatgagc aaattcgcca gctcgccatt gatgaaattg gcctgatgct
     7861 caagcgattt agcctctctg gctgggaacc gattctggtc aataaaacct cgcgcacaca
     7921 caaaaacagc gtctcgcgcc atgcgcaatt ttatgttagc cagatgctgg gctttattgc
     7981 cgaaaactat gaccaggcgc tgaccatcaa cgatgtggct gagcacgtca aacttaacgc
     8041 caactatgca atggggatat tccagcgggt catgcaattg acgatgaaac agtacattac
     8101 cgcgatgcgc atcaaccacg ttcgcgcgtt actgagcgat accgataaaa gtattctcga
     8161 tattgccctg acggcaggct ttcgttcaag tagtcgtttt tacagcacgt tcggcaaata
     8221 tgtcggcatg tcgccgcaac aataccgcaa acttagccaa cagcgccgcc agacgtttcc
     8281 cggctaaaga cgatatcagt atcagccaaa aaaatagttt agccgtcgat aataagcagt
     8341 gaaaataatt taaaataatc acataacata ttgtttataa aaacaaatat ttcaaaaatg
     8401 tttgtttttc acgcgcttta cagcccgaaa aggccggaag atacttgccc gcaacgaaga
     8461 ttccttcata accgggtaag caatgatgaa agtattaatt gttgaaagcg agtttctcca
     8521 tcaagacacc tgggtcggta acgctgttga gcgtctggca gatgctttaa gccagcaaaa
     8581 tgttaccgtg attaaatcca cctcctttga tgatggtttt gccattctct cttcaaacga
     8641 agccattgac tgcctgatgt tcagctatca aatggaacat ccggacgaac atcaaaacgt
     8701 cagacaattg atcggtaagc ttcatgagcg ccaacaaaac gtgccggtct tcctgttggg
     8761 cgatcgggaa aaagccctcg ccgcaatgga ccgggatctg ctggagcttg tcgatgaatt
     8821 cgcctggatt ctggaagata ccgccgactt tatcgccgga cgcgccgttg ccgcgatgac
     8881 ccgctaccgc cagcagctgt tgccgccact gttcagcgcg ctgatgaaat atagcgacat
     8941 ccatgaatat tcctgggcag cgccaggcca ccagggcggc gttggtttta ccaaaacacc
     9001 cgccggacgt ttctaccatg actactacgg tgaaaatctg ttccgcaccg acatgggcat
     9061 cgaacgaact tccctcggtt ctttgcttga ccatactggc gcatttggcg aaagcgaaaa
     9121 atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg gtagtcgtcg gtacttccgg
     9181 ctctaaccgc accatcatgc aggcttgcat gaccgataac gatgtcgtgg tcgttgaccg
     9241 taactgccat aaatccatcg aacaaggttt gatgctgaca ggcgcgaaac cggtctacat
     9301 ggtgccaagc cgcaaccgct acggcattat cgggccaatc tatccgcagg aaatgcaacc
     9361 tgaaaccttg cagaagaaaa tcagtgaaag cccgctgact aaagacaaag ccgggcaaaa
     9421 accgtcttac tgcgtggtga ccaactgcac ctatgacggc gtgtgttata acgctaaaga
     9481 agcgcaggat ctgctggaaa aaacctccga tcgtctgcac tttgacgaag cctggtacgg
     9541 ctatgcacgt ttcaacccga tctatgccga tcactatgcc atgcgcggcg aacctggcga
     9601 tcacaacggt cctaccgttt tcgccaccca ctccacccac aaactgctga atgcgctttc
     9661 acaggcctct tatattcatg tacgtgaagg tcgtggggcg attaacttct cccgcttcaa
     9721 ccaggcctac atgatgcatg ccaccacctc cccgctgtat gccatctgcg catccaacga
     9781 cgtggcggtg tcgatgatgg acggcaacag cggcctgtca ctgacgcagg aagtgatcga
     9841 cgaagcggtt gatttccgtc aggtgatggc gcggctgtat aaagagttcg ccgctgacgg
     9901 tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc gacccacaaa ccggcaaaac
     9961 ctatgacttt gctgacgcac caaccaaact gctgaccacc gttcaggact gctgggtaat
    10021 gcatccgggc gaaagctggc acggcttcaa agatattccg gataactgga gtatgctcga
    10081 cccgattaaa gtcagcatcc ttgctccggg aatgggtgaa gatggtgaac tggaagaaac
    10141 cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc cacggcattg tgcctacccg
    10201 caccactgac ttccaaatta tgttcctgtt ctctatgggc gtaacccgtg ggaaatgggg
    10261 gactctggtt aacacccttt gctccttcaa acgccactat gacgccaaca caccgctggc
    10321 gcaggtgatg ccggaacttg ttgaacaata tcctgacact tacgcgaaca tggggattca
    10381 cgatctgggt gacaccatgt ttgcctggct gaaagaaaac aaccctggcg cacggttgaa
    10441 cgaagcctat tccggcctgc cggtggcgga aatcaccccg cgtgaagcgt acaacgcgat
    10501 tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg ccaggacgta tcgcggcaaa
    10561 ctcagttatc ccgtatccgc caggaatccc gatgctgctg tctggtgaaa acttcggcga
    10621 taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa tcctgggacc atcatttccc
    10681 tggatttgaa cacgaaactg aagggactga aattattgac ggtatttacc acgttatgtg
    10741 tgtgaaagcg taaccactat tccgctgaag gcgtaattgt ttaaataaca ttacgccgcc
    10801 tggccttagg ccttttgagt atggcaacgt tttcataaaa attgctgcaa acaaaaatgt
    10861 catacttttt gcgcggcccc acc
//
LOCUS       DBFIFS010000153        10810 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.153, whole genome shotgun sequence.
ACCESSION   DBFIFS010000153 DBFIFS010000000
VERSION     DBFIFS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.153"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..483)
                     /gene="fliZ"
                     /locus_tag="N7A07_003426"
     CDS             complement(<1..483)
                     /gene="fliZ"
                     /locus_tag="N7A07_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU2478026.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     H"
     gene            complement(529..1248)
                     /gene="fliA"
                     /locus_tag="N7A07_003427"
                     /pseudo
     CDS             complement(529..1248)
                     /gene="fliA"
                     /locus_tag="N7A07_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(1568..3325)
                     /locus_tag="N7A07_003428"
     CDS             complement(1568..3325)
                     /locus_tag="N7A07_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HGU2478027.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            3591..4988
                     /gene="fliD"
                     /locus_tag="N7A07_003429"
     CDS             3591..4988
                     /gene="fliD"
                     /locus_tag="N7A07_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU2478028.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            5013..5423
                     /gene="fliS"
                     /locus_tag="N7A07_003430"
     CDS             5013..5423
                     /gene="fliS"
                     /locus_tag="N7A07_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU2478029.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            5423..5788
                     /gene="fliT"
                     /locus_tag="N7A07_003431"
     CDS             5423..5788
                     /gene="fliT"
                     /locus_tag="N7A07_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU2478030.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            5866..7353
                     /gene="amyA"
                     /locus_tag="N7A07_003432"
     CDS             5866..7353
                     /gene="amyA"
                     /locus_tag="N7A07_003432"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU2478031.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(7387..7800)
                     /gene="yedD"
                     /locus_tag="N7A07_003433"
     CDS             complement(7387..7800)
                     /gene="yedD"
                     /locus_tag="N7A07_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU2478032.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            7987..9192
                     /gene="yedE"
                     /locus_tag="N7A07_003434"
     CDS             7987..9192
                     /gene="yedE"
                     /locus_tag="N7A07_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU2478033.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            9189..9422
                     /gene="yedF"
                     /locus_tag="N7A07_003435"
     CDS             9189..9422
                     /gene="yedF"
                     /locus_tag="N7A07_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU2478034.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9531..10199
                     /locus_tag="N7A07_003436"
     CDS             9531..10199
                     /locus_tag="N7A07_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU2478035.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            10310..10789
                     /locus_tag="N7A07_003437"
     CDS             10310..10789
                     /locus_tag="N7A07_003437"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2478036.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         2649 a         2689 c         2744 g         2728 t
ORIGIN      
        1 gtgttgatac ttccgtaacg cgatgcggta attgttggtg ctggtggtgg gcagccacgg
       61 ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg agggaaatat tttgctcgtg
      121 tagatgattc cccaaacggc gcagacgaac gacatattca cgcaccgtac cagggctcat
      181 ttcagtttgc tcaaaaagaa attgcttaaa gccgataata tcgaaaaaat cactctgcgt
      241 tttgcaatgt aaatcaccgc aaaatcggca caatgccgcc catgattttt gttccgtttg
      301 ccagccattt tcatcaagca gcgtgtccag gcgggaaatc tcgattttat tcactatttt
      361 gccgtcgcga actaaggtaa tgcgatcgag caatttacgg caatgcgcgc aatgggtctg
      421 gctgtgttta aagtctttaa gatagcggct taatggccgt cttttcaggt gctgcaccat
      481 cattaagaac tcctggtagt caaagttaaa gtgcggcatt tactgacgtt ataacttacc
      541 cagtttagtg cgtaaccgtt taatagcctg gctgtgtaac tgactgaccc gcgattcccc
      601 gacctccagc actgcaccaa tctctttgag attcagctct tcctggtaat agagggttaa
      661 taccagtttt tcgcgctccg gcaacgtttc gatagcttcc atcacccgct ggcgcagatt
      721 actgtccagt agttgttgta gcgggttttc tcgttgatga tcatcagtaa ccaattcgat
      781 gctatcgccg tgctcttcgc gccactcatc gtaggagaag agctagctgt tattggtgtc
      841 gagcaacatt tggcgataat cggcaatatc gatccctaaa cgttccgcta cctcagtttc
      901 cgtggcgttg cggccaagtt cctgctccag ttgccctatt gcctgtgcca cttcacgcgc
      961 gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga agttcatcca gcatagcgcc
     1021 acggatacgc tgcactgcgt aagttgtaaa tgccgttcct tgtagggcgt catagcgttc
     1081 gacggcatta agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc
     1141 gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc agcgggacat aacgctgcca
     1201 cagcgagtgt ttatccatta caccttcagc ggtatagagt gaattcacga taaacagccc
     1261 tgcgttatat gagttatcgg catgattatc cgtttctgca gggtttttaa tcggacgatt
     1321 agtgggtgaa atgaggggtt atttgggggt tacaggtaaa ttccaggcag aaaaaacccc
     1381 gccggtggcg gggaagcacg ttgctgacaa attgcgcttt atgttgccgg atgcggcgta
     1441 aacgccttat ccggcctaca agaatgtgca aattcaataa attgcaattc aacttgtagg
     1501 cctgataagc gcagcgcatc aggcaatttg gcgttgccgt cagtctcagt taatcaggtt
     1561 acaacgatta accctgcagc agagacagaa cctgctgcgg tacctggtta gcttttgcca
     1621 gcacggagtt accggcctgc tgaatgatct gcgctttcga catgttggac acttcggtcg
     1681 catagtcggc gtcctgaata cgggactgcg cttcggacag gttggtagtg gtgttgttca
     1741 ggttggtgac tgcggaatcc agacggttct ggatagcacc cagggaagaa cggaatttgt
     1801 cgatggagct gattgcgtcg tccagggcag caagcgggtt ggtcgttgca gaaccagcac
     1861 tggtagtctc agtagtgatt ttacccgcgg agttcacatt tacagcagta ccaattgctg
     1921 gagcataatc tttattggta tcagtcgctg aagcataccc ggcaacagtc acagagccgt
     1981 tatccttgtt aacgctgtaa gagactgtat agtcggcaac gttagtgata ccacctttat
     2041 catccacata agacttcgca gcatcactgg attcacccgc ggtaaaccca atagttttgc
     2101 tcagtacacc ggaattaaag gtaattgaag atgtcgcggc agccgctttg gtttcgctca
     2161 atactacatc tttactcact gtagcctgat aagtaatccc accagggatt aacggagcta
     2221 ctggagtggt tgtcgcagga gttgcttttg cgatggtata ttctgtgcca ttgaatgtca
     2281 gagaggcacc gtcactacct tcgctcgctg ctttgagcag cgctttcata tcagctttag
     2341 ctgcgctacc agcgttgtta gtcgtcaagt tgcctgcatc gtctacgtat gcctggcttc
     2401 caccgatggt gatattacct gctgaatccg tttcgaaaga aacagtacca tcttttgtgg
     2461 tgtaagaacc attaacagat ttacccacat cagcatgaag ggtggcagct aacgcatcac
     2521 gttttgaagc tgaatcagca gcctgcgccg cggcgtctac acccgtacca gcagtagatt
     2581 tagtggtagt aaaatcacca gatttagcgt tgtaagtata atctacgccg ccaactgtga
     2641 ctttatcgcc attccctaat ttatcgaatg cagcatcggt agttaacaag gtattttcgg
     2701 ttttcagatc ataaagacct gtcgtggtgt ttaacttcgc gccagcagaa gttaaatcac
     2761 ttaccgttgc agctttgttg gtaatagtac ctttaccatt tacgttaaag ccattcagac
     2821 ccagagtatc agaatcgatt tttttcaggt cgatcgtgat ggtttcgccg tcattcgcac
     2881 caacctgaat tttcattgaa ccgtctttcg ccagcacgtt cacgccgttg aactgggtct
     2941 ggccggatac gcggtcaatt tcatcaagac gagatttgat ttcgtcctgg atggagtcca
     3001 ggtcagaatc ggagttagtc cctgtagtgg cctgaaccgt cagttcacga atacgctgta
     3061 agttgttgtt gatttcggac agcgcgcctt cggtggtctg cgcaacagaa ataccgtcgt
     3121 tggcgttacg ggccgcctga gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg
     3181 cctgacctgc ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga
     3241 tagaactcga cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc
     3301 tgttggtatt aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg
     3361 tattgggttt ccacccgtcg gctcaatcgc cgtcaaacct gttatcgtct gtcgtaaaac
     3421 aacctttaga atttttttca caaacagcca ttttttgtta gtcgccgaaa tactcttttc
     3481 tctgcccctt attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcagg
     3541 ccgataaccc cggtattcgt tttacgtgtc gaatgataaa aggaaatcgc atggcaagta
     3601 tttcatcgct gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg
     3661 ccgcgcaaaa agcgacgcta acccccattt caaatcagca atcgtcgttt accgctaaac
     3721 ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat
     3781 tgtctaaagc cgatcttttt tccgccacca gcagcaccac cgcgttcagt gccaccaccg
     3841 cgggtaacgc catcgccggg aaatacacca tcagcgtcac ccatctggcg caggcgcaaa
     3901 ccctgaccac gcgcaccacc agagacgata cgaaaacggc gatcgccacc agcgacagta
     3961 aactcaccat tcaacaaggc ggcgacaaag atccgattac cattgatatc agcgcggcta
     4021 actcgtcgtt aagcgggatc cgtgatgcca tcaacaacgc aaaagcaggt gttagcgcga
     4081 gcattattaa cgtgggtaac ggtgattatc gtctgtcagt cacatcaaat gacaccggcc
     4141 ttgacaatgc gatgacgctc tcggttagcg gtgatgatgc gctacaaagt tttatgggct
     4201 atgacgccag tgccagcagc aacggcatgg aggtctcggt cgccgcccag aatgcgcagc
     4261 tgacggtcaa caacgtcgcc attgagaaca gcagcaacac catcagcgac gcgctggaaa
     4321 acatcaccct gaacctgaac gatgtcacca cgggcaacca gacgctaacc atcactcagg
     4381 acacctccaa agcgcaaacg gcgattaaag attgggtgaa tgcctataac tcgctaatag
     4441 ataccttcag cagcctgacc aaatacaccg ccgtagatgc gggagctgat agccagagtt
     4501 ctagcaatgg cgcactgctc ggcgactcca cgctgcggac gattcagacg cagttgaagt
     4561 cgatgctgag taataccgtc agttcttcca gctataaaac gttggcgcag attggtatca
     4621 cgaccgatcc cagcgatggc aaactggaac tggatgccga caaactcacc gctgcactga
     4681 aaaaagatgc cagcggcgta ggtgcattga ttgttggcga tggtaaaaaa accggcatca
     4741 cgaccaccat cggcagcaac ctgaccagtt ggctttcgac aacgggcatt attaaagccg
     4801 ctaccgatgg cgttagtaag accctgaata aattaactaa agactacaac gccgccagcg
     4861 atcgcattga cgcgcaggtc gcgcgctaca aagaacaatt tacccaactg gacgttttaa
     4921 tgacctcgtt aaacagcaca agcagctact taacgcagca gttcgaaaac aacagtaatt
     4981 ccaagtaatc attattcatc gggagacagg tcatgtacgc ggcaaaaggc acccaagcct
     5041 atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag ctggtcacca
     5101 tgctatttga tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca
     5161 atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacggactgc
     5221 gggtgagtct tgatgaagag agcaaagacg aactaaccca aaacttgatt gctctttata
     5281 gctatatggt caggcgtttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag
     5341 aagtggaaac attaatgcgc aatattgccg atgcctggaa agagtcgtta ctctcccctt
     5401 ctttgattca ggacccagtc tgatgaacca tgcaccgcat ttatatttcg cctggcaaca
     5461 actcgtcgaa aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact
     5521 catcgccagc gaaatggcgt atgttaatgc ggtgcaggag attgcacatt tgactgaaga
     5581 ggttgacccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga
     5641 caacgaaagc aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt
     5701 cggtcagtca tcggtgcaaa aatcggtgtt gagtgcctat ggcgatcagg gcggctttgt
     5761 gctggctccg caggataacc tcttttgaat ctgaatgagt cgatggctcg cgaataatcc
     5821 gattacggct acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg
     5881 ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc tgaattggcc
     5941 gagcgcgccg acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat
     6001 aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggt
     6061 gagtttgatc agaaaggcag catccctact aaatatggcg ataaagtaca actgctggcc
     6121 gccattgacg ccctgaaacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac
     6181 aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gcgttaatgc tgatgaccgt
     6241 acgcaaattg atgaagaaat cattgaatgc gaaggctgga cgcgttacac cttccccgct
     6301 cgtgccgggc aatactcaca gtttatctgg gatttcaaat gttttagcgg tatcgaccat
     6361 atcgaaaacc ccaatgaaga tggcattttt aaaattgtta acgactacac cggcgaaggc
     6421 tggaacgatc aggttgatga tgaactcggt aatttcgatt atctgatggg cgagaatatc
     6481 gattttcgca atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa
     6541 caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac atattccagc ctggttttat
     6601 aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcggag
     6661 tactggtcgc atgaagttga taagctgcaa acgtatattg atcaggtgga aggcaaaacc
     6721 atgctgtttg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat gggacgcaac
     6781 tacgacatga cgcagatttt cactgggacg ctggtggaag ccgatccttt tcacgccgta
     6841 accctcgttg ccaaccacga cacccaaccg ttgcaagccc tcgaagcacc ggtcgaaccg
     6901 tggtttaaac cgctggcgta tgctctaatt ttgttgcggg aaaatggcgt tccttcggtg
     6961 ttctatccgg acctctacgg tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat
     7021 ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc
     7081 gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt
     7141 ggcaccgacg aatatcccgg ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa
     7201 accattcatt taggagagaa ttacggcaat aaaacctggc gtgattttct tgggaaccgg
     7261 caagagagtg tagtaaccga cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc
     7321 gttagcgtgt gggttatcga agaggtgctt taaattcatc cccggcggca agccggggag
     7381 atttcattac ggcaatggcg tcggtaacgg cgctttatcc agcgcagcgg cacactctgc
     7441 cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg
     7501 ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt
     7561 caacttgccc ggcacagcaa tcacccgctg ccactgacgg caatctaggg tatcgccctc
     7621 tttagtgaca atcaggctgc ctatcgcttc cgggctgacc agcgcacgtt gcggcccttt
     7681 ggtctgccag tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt
     7741 ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat
     7801 cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc
     7861 tttccaggtt cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa
     7921 ataaatttgc atggtgtatg attcgcgact cttgtttcaa accttctgag ttcagaggct
     7981 acattcatgt catggcagca attcaaacac gcctggttga ttaaattctg ggcgcccatc
     8041 cccgcggtca tcgcggcggg tattctctcc acttactatt ttggcatcac tggcaccttt
     8101 tgggctgtca cgggtgaatt tacccgttgg ggcggtcagc tcctgcagct gttcggcgtc
     8161 catgctgaag agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgc
     8221 atcgacggga tgatgatcct cggtatgttt ggcggctgct ttgccgcagc gctgtgggcc
     8281 aacaatgtca aactacgcat gccgcgtagc cgtatccgca ttatgcaggc cattattggc
     8341 ggcattatcg ccggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcattcttt
     8401 accggtattc ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt
     8461 tcatggtttg gcgcgcgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag
     8521 aaagttgctg ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt
     8581 ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc tgctcacagc gatgaaccaa
     8641 cccaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg
     8701 caaatctgct ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg
     8761 aaagcaatca ttattggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta
     8821 ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt
     8881 ggttttggca tcgtgctggc tggcggctgc gaaaccggct ggatgtaccg cgcggtagaa
     8941 ggccaggtgc actactggtg ggtcggtctg ggcaatgtga tcggctcaac gattctggcg
     9001 tattactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat caacttgctg
     9061 aaaacctttg gtccgatggg gggcctgctg gtgacatatt tgctgttgtt tgctgcgctg
     9121 atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag
     9181 gagatcgcat gaaaaatatc gttcctgatt accgccttga tatggtgggt gaaccctgcc
     9241 cttatccggc agtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg
     9301 aagtggtgag cgactgtccg cagtcgatca ataatattcc actggatgcg cgtaatcacg
     9361 gctatacggt gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt
     9421 aatccctcat tcccgctgtg acccacagcg ggaacatcgt tacctttgta tcacatagat
     9481 tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggtttct atgtgtggac
     9541 gctttgccca atcccaaacg cgtgaagatt accttgcgct tctcgcggaa gatattgaac
     9601 gcgatattcc ctatgatccc gaacccattg gcagatacaa cgtcgcgccg ggaaccaaag
     9661 tcctactgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg
     9721 ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gcggcaacca
     9781 gtcgtatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt
     9841 ttgagtggaa aaaagaaggc gacaaaaagc agccttattt tatctatcgt gctgatggac
     9901 aacctgtttt tatggcagcg ataggcagca caccgttcga gcgcggtgac gaagccgaag
     9961 gatttttgat tgtcactgca gcagcagacc agggtctggt agatattcat gaccgccgcc
    10021 cgctggttct gtcgccagaa gctgcgcggg aatggatgcg gcaagagatt agcggtaaag
    10081 aagcctcaga aatcgcggcc agtggctgtg ttccagcaaa ccagttctcc tggcacccgg
    10141 tatcgcgcgc ggtgggtaat gttaaaaacc agggtgcgga gttaattcaa cctgtttgat
    10201 aagcctggag attattgatc aacaatactg cgtcataagt aatctctatt agacaaagat
    10261 ttcattacct gttggcatat tgcaaaaata acaccaatac ggaatcgtca tgttcacgat
    10321 taaaacagat gatctcaccc atccagcagt gcaagcatta gtggcttacc atatttccgg
    10381 catgctgcag cagtctcccc ctgaaagcag tcatgcttta gacgtgcaaa aattacgtaa
    10441 cccgacagtg acattctggt cagtatggga aggcgaacaa ctcgcaggaa ttggtgcgct
    10501 gaagttgctg gatgataaac atggcgaact gaaatcaatg cggaccgcgc caaattattt
    10561 acgtcggggt gtcgccagtc tgattttacg ccacattttg caggtcgccc atgacagatg
    10621 ccttcatcgc ctgagtttag aaacgggtac acaggctgga tttacggcct gccatcaact
    10681 ttatttgaag catggtttcg ttgattgcga accgtttgcc gattatcaac ttgatccaca
    10741 cagtcgattt ttgtcattga cgctatgcga agataatgag ttgctttgag ccagacgcag
    10801 cacattcttg
//
LOCUS       DBFIFS010000154        10444 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.154, whole genome shotgun sequence.
ACCESSION   DBFIFS010000154 DBFIFS010000000
VERSION     DBFIFS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.154"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            40..1396
                     /gene="dcuC"
                     /locus_tag="N7A07_003438"
                     /pseudo
     CDS             40..1396
                     /gene="dcuC"
                     /locus_tag="N7A07_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1439..1936)
                     /gene="sspB"
                     /locus_tag="N7A07_003439"
     CDS             complement(1439..1936)
                     /gene="sspB"
                     /locus_tag="N7A07_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU2478037.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1942..2580)
                     /gene="sspA"
                     /locus_tag="N7A07_003440"
     CDS             complement(1942..2580)
                     /gene="sspA"
                     /locus_tag="N7A07_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU2478038.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2975..3367)
                     /gene="rpsI"
                     /locus_tag="N7A07_003441"
     CDS             complement(2975..3367)
                     /gene="rpsI"
                     /locus_tag="N7A07_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU2478039.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3383..3811)
                     /gene="rplM"
                     /locus_tag="N7A07_003442"
     CDS             complement(3383..3811)
                     /gene="rplM"
                     /locus_tag="N7A07_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU2478040.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4030..5157)
                     /gene="zapE"
                     /locus_tag="N7A07_003443"
     CDS             complement(4030..5157)
                     /gene="zapE"
                     /locus_tag="N7A07_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU2478041.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5351..5749
                     /gene="zapG"
                     /locus_tag="N7A07_003444"
     CDS             5351..5749
                     /gene="zapG"
                     /locus_tag="N7A07_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU2478042.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5903..7270
                     /gene="degQ"
                     /locus_tag="N7A07_003445"
     CDS             5903..7270
                     /gene="degQ"
                     /locus_tag="N7A07_003445"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU2478043.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7360..8427
                     /gene="degS"
                     /locus_tag="N7A07_003446"
     CDS             7360..8427
                     /gene="degS"
                     /locus_tag="N7A07_003446"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU2478044.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8491..9429)
                     /gene="mdh"
                     /locus_tag="N7A07_003447"
     CDS             complement(8491..9429)
                     /gene="mdh"
                     /locus_tag="N7A07_003447"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU2478045.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9864..10334
                     /gene="argR"
                     /locus_tag="N7A07_003448"
     CDS             9864..10334
                     /gene="argR"
                     /locus_tag="N7A07_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU2478046.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2556 a         2662 c         2618 g         2608 t
ORIGIN      
        1 tatgggattg acccttttct tttgacacgg agttcaacaa tgttcggcat aattatatct
       61 gtcatcgtat taattacgat gggctatttg atcctgaaaa actacaaacc tcaggtggtg
      121 ctggctgcag gtatcttcct gatgatgtgc ggtgtctggt tagggttagg tggtgtactc
      181 gatcccgcca aaagcagcgg ctacttgatc gtcgatattt ataatgaaat cctgcgcatg
      241 ctgtccaacc gcattgccgg attggggctg tcgattatgg cggtgggcgg ttatgcccgc
      301 tacatggagc gcacaggagc cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa
      361 ctcattcgct cgccgtatat tattctgtcg gcaacttacg tcatcggcca aatcatggcg
      421 cagtttatta ccagcgcctc cggcctgggt atgttgctga tggtcacctt atttccgaca
      481 ctggtgagtc tgggagtaag ccgtctctct gcggtggcgg ttatcgcaac cacaatgtcc
      541 attgagtggg ggattctgga aacgaactcc atttttgcag cccaggtagc gggaatgaaa
      601 attgccactt acttcttcca ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg
      661 gcgatctccc actttttcgt gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac
      721 gaacaggcag agcaaaaagc tctcgataat gtcccgccgc tctattacgc cattttacct
      781 gtgatgccgt taatcctgat gctcggctcg ctattccttg cccacatcgg gctgatgcag
      841 tcagaactgc atctggtggt ggtgatgtta ctgagtttga cgatgtttgt tgagttcttc
      901 cgcaagcata acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt
      961 acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta
     1021 acgacgattg gcactgtcga tgcggttatc aggggtgcgg agcattctgg tctgggcggt
     1081 attggcgtga tgattattat ggcgctggtc attgccattt gtgccattgt gatgggctct
     1141 ggcaatgcgc cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat
     1201 gtaccagcgg ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcggtttc
     1261 gccgattact gcggtggtgg tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt
     1321 ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga ttgccacaat
     1381 cacgctattt tattaagtca ttaaaagaca aaacaggccg ccagggcctg tttttgtatt
     1441 acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggtggt tcatcgtcag
     1501 gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata acggtttcgt
     1561 tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag gcagcttcag
     1621 gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg
     1681 gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg
     1741 ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg
     1801 catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga
     1861 gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgtta
     1921 gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt ctgcttcagt
     1981 taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt tcagctcttt
     2041 cgcacccggg ccgctgaact cgatacccag ttgcggcaga cgccacagca gcggagcaag
     2101 gtagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg
     2161 cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt cagaagctga
     2221 accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat gcatgtacaa
     2281 acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc
     2341 atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt
     2401 cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac
     2461 gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata
     2521 gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat
     2581 gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga
     2641 agttgtgtta cccagtaagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca
     2701 atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg acctttgtgg
     2761 tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag
     2821 tcccttattg gcgatgtggt ttttgttttg cctgtctgtc agacggcagc aaaaagcaac
     2881 tttccatttt ttacgctgat tcagaattta accataaaaa aacccgccga agcgggtttt
     2941 ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac
     3001 gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga
     3061 agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga
     3121 taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca
     3181 ggtccagttt ctcaaccata tcgaccagtt ccagcggctg acgaactacc atgcgagcag
     3241 tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg
     3301 gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc agtgccgtag tattgatttt
     3361 cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt gccgcgtggt
     3421 tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt
     3481 ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct
     3541 cttcaaaggt cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt
     3601 cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac
     3661 cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt
     3721 cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta
     3781 cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa tagttacacg
     3841 ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct
     3901 accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa
     3961 cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg
     4021 tggcaggttt taacccgcta aatgctcgcg cttcagatac tcttcgcttt gcatctcttg
     4081 cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat aaatttcata
     4141 cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact catccaccag
     4201 cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta catcaaacaa
     4261 catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc gggcgtcgac
     4321 gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg tcgctaatgg
     4381 ccgatggttg atttctaacg tcggtgaatt ttctcgtttc ccccccgcca gcgccaacca
     4441 tagtttatcc atttgcgccc gggtttcatc gtggagtggc gaaagccaca gatgcgcctg
     4501 agtgagtgta cgcagacgat aatcaacacc agcgtccacg ttcattacat cacaatgctg
     4561 tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aagccatttc gataaagttc
     4621 gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca gggctttcat
     4681 caggccgcca agtagcatgg catcggtaat atcagagaca aaaaattcgt caaaacagag
     4741 cacgtcagtt tctgctttaa aacgatcagc aataatttcc agcggatcgg tctgcccctg
     4801 taaggcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca ggcgctgttt
     4861 ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc cgcgtcctac
     4921 accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttccc gtttacccca
     4981 cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg tgctattgat
     5041 gagttcctga taaataattt ccaagcggct gacggcctct ttttgaacgt cgtcgggttg
     5101 atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggagtaacgc tttgcatgat
     5161 cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc
     5221 gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac ggttatagtg
     5281 gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca aaagacaacg
     5341 ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca tcattattgg
     5401 tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt tgcagtacga
     5461 actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta gccactttgc
     5521 ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt atcagcacat
     5581 ggcgaaaagc tccagcagcc tgctgccgga actgtctgct gaagcaaacc cgttccgtaa
     5641 tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc ctcgcgacta
     5701 ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat ttatttttcg
     5761 ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa tctggtgtct
     5821 cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct tttcttatca
     5881 ttcaggtacg agagcaggaa taatgaaaaa acaaacccag ctgttgagtg cattagcgtt
     5941 aagtgtcggg ttaactctct cggcgtcatt tcaggccgtt gcgtcgatcc caggccaggt
     6001 tgccgatcag gcccccctcc ccagtctggc tccaatgctg gaaaaagtgc ttccggcagt
     6061 ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg aagaattcaa
     6121 aaagtttttt ggtgatgatt taccggatca acctgcacaa cccttcgaag gtttaggctc
     6181 cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg tgattaatca
     6241 ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa aactgattgg
     6301 tagcgatgac cagagcgata tcgccctgtt gcaaattcaa aacccgagca aattaacgca
     6361 aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtgg cggtcggtaa
     6421 cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag gacgcagcgg
     6481 gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta accgcggtaa
     6541 ctccggcggt gcactgttaa accttaacgg tgagttaatt ggcatcaaca ctgcaatcct
     6601 tgcgcctggc ggcgggagcg tcggaattgg atttgccatc cccagtaata tggcgcgaac
     6661 actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag gcatcaaagg
     6721 caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt
     6781 tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaaa cgggcgatat
     6841 tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc
     6901 gaccaccgag ccgggcacga aagtgaagct tggcctgctg cgtaacggca aaccactgga
     6961 agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc
     7021 agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca aaggtattaa
     7081 aatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa aagacgatgt
     7141 gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc
     7201 ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca tctatctgct
     7261 gatgcgttaa tgtcgtaaac cgggcatcag gctgacgtgt gatgtccggt taactcgtgg
     7321 tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa gctcttacgt
     7381 tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc
     7441 agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc tgccagttat
     7501 aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg tggtttgaac
     7561 accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat catggatcaa
     7621 cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat catcgtcgcc
     7681 ttacaggatg gacgtgtatt tgaagcattg ctggtgggat ctgactctct aaccgatctg
     7741 gcggtactta aaattaatgc cactggcggt ttacctacca ttccaattaa tgcacgtcgc
     7801 gtaccgcaca ttggcgacgt ggtactggcg atcggtaacc cgtacaacct cgggcagacc
     7861 attacccagg ggattattag tgctacgggt cgaatcggtc tgaacccgac cgggcggcaa
     7921 aacttcctac aaaccgatgc ttccattaac cacggtaact ctggcggcgc gctggtgaac
     7981 tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa
     8041 acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat tatggataag
     8101 ctgatccgcg atggtcgcgt gatccgcggc tacattggta tcggcggacg cgagatcgca
     8161 ccactgcacg cgcagggcgg tggtatagat caactgcaag ggatcgtggt taatgaagtg
     8221 tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat tatttcggtg
     8281 gataacaaac cggccatctc tgctctggag acgatggatc aggtggcaga aattcgccct
     8341 ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct gcaggtcacc
     8401 attcaggaat atccggcaac caattaaggc cagcgtgaaa gacaaaaaaa ccggagtctg
     8461 tgctccggtt ttttattatc cgctaatcaa ttacttatta acgaactctt cgcccagggc
     8521 gatatctttc ttgagcgtat ccagcatacc ttccagcgcg ttctgttcaa atgcgctcag
     8581 ggtaccgata gatttacgct cttccacgcc gtttttaccc agcagaagcg gctgagagaa
     8641 gaaacgggcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc
     8701 ctgcagtgcg cgaaccagag acaggccaaa acgtgcagca gcctggccca tagacagggt
     8761 tgccgacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt
     8821 ggtcagatca gccacttcct gctcggtaaa actaacgcca ggaacctgtg acaacagcgg
     8881 cagaatggta acaccagagt gaccgccaat aaccggcact tcaacttcgc ctggctgttt
     8941 gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa
     9001 cagtttgttt ttgtcataaa caccggcttt tttcagcact tcagcagcaa tcgcaactgt
     9061 ggtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg
     9121 ctgtaccagg tttttcacga tgccggcgtt aacattaaac aggtcggaac gatccatacc
     9181 cggtttacgc gctacacctg cagagataag aacgacatct gcgccttcca gcgccggagt
     9241 cgcatcttca ccagaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac
     9301 ggccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag
     9361 ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc
     9421 gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc
     9481 gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc
     9541 aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaagttacg taattaacaa
     9601 tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta ccgttcagcc
     9661 aaaacaacat caatttgata acaattaatt tacttttaag cagaatttgc atgccgtgac
     9721 gcaggcatat ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc
     9781 agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa taatgttgta
     9841 tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag
     9901 catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt
     9961 tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt
    10021 ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac
    10081 tgggtgtacc aaccacctcc agtccattga aaaatctggt gctggatatc gactacaacg
    10141 atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct cgcctgctgg
    10201 actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta
    10261 ccacccctgc taacggtttc accgtcaaag acctgtatga agcgatttta gagctgttcg
    10321 accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttatccc
    10381 tctcaacccc ctgctttccc ctgcgattaa tttaacgaat agtgcgtttt actgcgacat
    10441 gtca
//
LOCUS       DBFIFS010000155        10334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.155, whole genome shotgun sequence.
ACCESSION   DBFIFS010000155 DBFIFS010000000
VERSION     DBFIFS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.155"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1671)
                     /gene="rhsC"
                     /locus_tag="N7A07_003449"
     CDS             complement(<1..1671)
                     /gene="rhsC"
                     /locus_tag="N7A07_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HGU2478047.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRF"
     gene            complement(1831..2043)
                     /gene="rpmE"
                     /locus_tag="N7A07_003450"
     CDS             complement(1831..2043)
                     /gene="rpmE"
                     /locus_tag="N7A07_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU2478048.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            2246..4444
                     /gene="priA"
                     /locus_tag="N7A07_003451"
     CDS             2246..4444
                     /gene="priA"
                     /locus_tag="N7A07_003451"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU2478049.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4600..5625
                     /gene="cytR"
                     /locus_tag="N7A07_003452"
     CDS             4600..5625
                     /gene="cytR"
                     /locus_tag="N7A07_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU2478050.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            5717..6676
                     /gene="ftsN"
                     /locus_tag="N7A07_003453"
     CDS             5717..6676
                     /gene="ftsN"
                     /locus_tag="N7A07_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU2478051.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6769..7299
                     /gene="hslV"
                     /locus_tag="N7A07_003454"
     CDS             6769..7299
                     /gene="hslV"
                     /locus_tag="N7A07_003454"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU2478052.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            7309..8640
                     /gene="hslU"
                     /locus_tag="N7A07_003455"
     CDS             7309..8640
                     /gene="hslU"
                     /locus_tag="N7A07_003455"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU2478053.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            8707..9633
                     /gene="menA"
                     /locus_tag="N7A07_003456"
     CDS             8707..9633
                     /gene="menA"
                     /locus_tag="N7A07_003456"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU2478054.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            9726..10211
                     /gene="rraA"
                     /locus_tag="N7A07_003457"
     CDS             9726..10211
                     /gene="rraA"
                     /locus_tag="N7A07_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU2478055.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            10271..>10334
                     /locus_tag="N7A07_003458"
     CDS             10271..>10334
                     /locus_tag="N7A07_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478056.1"
                     /translation="MQNIILLFIYLLLIAVNHFRY"
BASE COUNT         2395 a         2779 c         2858 g         2302 t
ORIGIN      
        1 gaagcggtca tggtcataac gggttacata accggaacag tcggtgaagc tcagcaactg
       61 accgtaacgg ctccacgtca tggttttccg gctgccggtg gcatcttccg ttgcgcaggg
      121 taagtcactg tgtggattat cataacggta gcgggtgata tcgccgtcag gggcagtttc
      181 ctgaatcaga cggccccatt catcatattc ccggcgtatt tccagcccgt caggcccggt
      241 ggctgacgtt aactggctgt ggtggttata gtaaaacgcc gatgccctgc catccggcgt
      301 ggtgatgcgc gtgatgaggc ccgtcaccac atccgggctg tattctgttg tcctgcctgc
      361 ggcatccgtc tgtgccctga gcctgcccac cgcgtcaaac tgactctgcg tgacgctgcc
      421 gtccgcgtgt tcctttttca ccacccgctt cagcccgcct tcaccctgcg tgtgcaggac
      481 ttcacggcgg ttcaggctgt cggtgatggt gatgcggtct ttctcatact gatacgtgta
      541 gcttaagcct gccgggttta gctgttctgt cacccgcccg tcgctgtcgt aacggtaacg
      601 gatttccggc cggcccgtgt gacggtgcgc caccatccgg ccccggtatt tatcatcgta
      661 agtaaagctg cgcacctgtt tgccactacg gtcatacacc accgccagtt cgccgcgcgg
      721 cgtccagcca tagcgcacca gcggcgcggc aggtaaattc tccgggtatt ccgggtcgtg
      781 cgtcagccac acggcagaca gacggatgcc gttgtcccgg ccatattcgg tgtaacccgg
      841 cagggtatca ggaaaagcgg acggttccgt cccgccggaa atggcctgct gccgggcttc
      901 ttctgcccgc tgcgcctgcg tggtcagtac cagccggaag tgacgcccgg caccatccgt
      961 cacgccggtg atttcgccgc tgaattcacc ggcggcttcg cggtggaacg tctgtgtgcg
     1021 cccgaagcgg tccaccagcc cggtcagtac ccggtacggc ggcagcggcg caggcagcac
     1081 ctcatccgct tccggcaccc gctcacacca gccgagcagc caccacggcc cctgcggact
     1141 gtttgtcgcc agataacgat gcggacttaa gcggagttct tccggcagcg cctgccagag
     1201 tgcggccagc cggtgacctt catccagttt cgccacgccg ccgcgcacca gccagagtga
     1261 ctcgctgcgg ctgtaaccgt cctcaccggg aaacaggtgc tcaaaataca ggcttctgcc
     1321 gccgttatca ctgagtatca gtgtgttatc gcgcagctgt aagcggatat ccgcaggcat
     1381 tttccagccg gggccgaggc tccccaccgg cgcgggcgtt tttgtccggt aactgctgta
     1441 ggtgcgggag aggatgaacg gcagcgggcc gggcagggcg atgtcggttt caccgggaag
     1501 gacctttgca ccgagcaggg gattgaccgg atggccggac gtcactccgc cggggcacac
     1561 cgaacaggcc acgccggtgg gggcaccaat gcgcaccccg gctgaaccct gaacaatgct
     1621 accgccatac tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tcgtaaaatc
     1681 cttttgaaat aaataagaac cagaagccta gcgccagtca acaacaggaa atgataatga
     1741 gttaacaccc cggaattttt cagaatttat aaaaggcaaa ttacaggcac aaaaaaagcg
     1801 ccgtgcggcg cttttttcgg aaatccggtc ttatttgctg cccgggatgt tgaaacgctt
     1861 gttgaagcgg tcaacacggc caccggtagc aacatcacgc tgtttgccag tgaagaacgg
     1921 gtggcacttg ctgcacacgt cgaggttcag gtcatgacca acggtggagc ggattttcat
     1981 tacgttaccg caagagcagc tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt
     2041 catgggaaaa cctcggttta aggccgcgtc gctcttccag ccctaacgcc agacaccacg
     2101 cgatgttaaa agtatagctt caatacgatc atttcgtacg aaggcgcgaa atcatacaga
     2161 aattaaccag catatgcaaa ctgatccgca ctcttctacg gcaatgtgta tactaaccca
     2221 ccgaatttca agtcaggatg atgctatgcc cgttgcccac gttgccttgc ccgttccgct
     2281 tcctcgtacc tttgactatc tcctgccaga aggcatggcg gttaaagccg ggtgtcgcgt
     2341 gcgcgtgccg tttggcaaac agcaggagcg catcggggtt gtggtgtcag ttagcgacgt
     2401 cagtgagctg ccgcttaacg agctaaaagc ggtagtcgaa gtgctggata gtgagccggt
     2461 gtttactcac tccgtctggc gattgctgct ctgggcggcg gattactatc atcatccgat
     2521 tggcgatgtg ctgtttcatg ccttgccgat tttactgcgc caggggcggc ctgcggcgaa
     2581 cgcgccgatg tggtactggt ttgccactga acaaggccat gcggtggatc tgaacagcct
     2641 gaaacgttcg ccaaagcaac aacaggcact ggcggcattg cggcaaggca aaatctggcg
     2701 cgaccaggtt gccacgcttg aatttaatga tgccgcgtta caggcgctgc gcaaaaaagg
     2761 tctgtgtgat ttagcaagtg aaacaccaga gtttagcgac tggcgaacga actatgccgt
     2821 ttctggtgaa cggttgcgat tgaataccga acaggccacc gccgttggcg caattcatag
     2881 cgcggcggat accttctctg cttggctgct ggcgggcgtt acaggttccg gtaaaacaga
     2941 ggtttatttg agcgtactgg aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt
     3001 gccggaaatc ggcctgacac cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcccc
     3061 cgtagaagtt ctgcattccg gcctgaacga cagcgagcgt ctttcggcgt ggttgaaagc
     3121 gaaaaatggt gaggcggcga ttgtcatcgg cacccgctcc gcgctgttta cgccgtttaa
     3181 aaatctcggt gtgattgtca ttgatgaaga gcacgacagt tcctacaagc agcaggaagg
     3241 ctggcgctat catgcccgcg acctggcggt ttatcgtgcg cacagcgagc aaatcccgat
     3301 tattcttggc tccgcaacac ccgcactgga aacgttatgc aacgtccagc agaaaaaata
     3361 ccgcctgctg cgcctgacgc gtcgggcggg gaatgcgcgt ccggcaattc aacatgtgct
     3421 ggatttaaaa ggtcagaagg tgcaggcggg gctggctccg gcgttaatca cccgtatgcg
     3481 ccagcattta caggccgaca accaggttat cctctttctt aaccgccgtg gctttgcgcc
     3541 tgccttgctg tgccacgact gcggctggat tgccgaatgt ccacgttgcg atcactacta
     3601 cacgctgcat caggcgcaac agcatttgcg ctgtcaccac tgtgacagcc aacgcccggt
     3661 gccgcgccag tgcccttcct gcggttccac gcacctggta cccgtggggc tgggcaccga
     3721 acagcttgaa cagacgctcg cgccgttatt ccctggtgtg cccatttctc gtatcgaccg
     3781 cgataccacc agccgcaaag gagcgctgga acaacaactg gcggaagtgc atcgcggcgg
     3841 cgcgaggata ttgattggca cgcaaatgct ggcgaaaggt caccactttc cggatgtgac
     3901 gctggtagcg ttactggatg tggacggcgc gctgttttct gccgattttc gctcggcaga
     3961 gcgttttgcc cagctataca cccaggtagc aggccgtgcc gggcgtgcgg gtaaacaggg
     4021 cgaagtggtg ctgcaaacgc atcatccgga acacccgcta ttgcaaacgt tgctctataa
     4081 aggctacgac gcttttgccg aacaggcact ggctgagcgg cgaatgatgc aactgccgcc
     4141 gtggaccagc catgtgattg tgcgtgcgga agatcataac aatcagcacg cgccattgtt
     4201 cctgcaacaa ctgcgtaatc tgatcctctc cagcccgctg gcagacgaca aattgtgggt
     4261 tctcggtccg gttccggcac tggcacctaa acgtggcggt cgctggcgct ggcagatatt
     4321 gttgcagcac ccttcccgcg tgcgcttgca acacatcatt aacggtacgc tggcactcat
     4381 caatacaata ccggattccc gtaaggtgaa atgggtgctg gatgttgatc cgattgaggg
     4441 ttaaaccgct cacgatgcga ggcggatcga aaaattcaat attcatcaca cttttcatga
     4501 aaattctgta accgttttca cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca
     4561 tggatgtagt acgcctgacg tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa
     4621 actgccgcga ccatgaaaga cgttgccctc aaggcaaaag tctctacagc gaccgtctcc
     4681 cgagcattaa tgaatcccga taaagtctcc caagccaccc gtaatcgggt tgaaaaagcg
     4741 gcccgtgaag tgggttattt accgcagcct atggggcgca acgtcaagcg taatgaatcc
     4801 cgcaccattc tggtgattgt cccggatatc tgcgatccct tctttagcga aattattcgc
     4861 ggtatcgaag ttacggcggc aaatcacgga tatctggtgc tgattggcga ctgtgcgcat
     4921 caaaatcagc aggaaaaaac ctttatcgat ttgatcatca ccaagcaaat tgatggcatg
     4981 ttgctgctgg gttcaaggct gccgtttgat gccagcattg aggaacagcg taatctgccg
     5041 ccgatggtga tggcgaacga atttgcaccg gaactggagc tgcctaccgt tcatatcgac
     5101 aatctgaccg ccgcatttga tgcagtaaat tatttatatg agcaagggca taaacggatt
     5161 ggctgtatag ccggtcccga agagatgccg ctgtgtcact accgcctgca aggttacgtt
     5221 caggcgctgc gtcgctgcgg cattatggtt gatccgcaat acatcgcccg tggcgacttc
     5281 accttcgaag ccggaagcaa agcgatgcag cagctgcttg atcttccaca accgcctact
     5341 gctgtcttct gccatagcga tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag
     5401 gggctgaaag tcccggaaga cctttccata atcggttttg ataacatcga cctgacgcaa
     5461 ttttgtgatc cgccgctgac aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct
     5521 atgctgttat tgcttgatca aatgcagggg caacacgttg gcagtggctc tcgtttaatg
     5581 gactgcgaac ttatcatccg gggatcaaca cgcgcgttac cttaaagtaa acctttaaga
     5641 ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg gcgggctgac gaacgaataa
     5701 atacagcgaa acgatagtgg cacaacgaga ttatgtacgc cgcagccaac cggcaccttc
     5761 gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc
     5821 tatggtcgct attgctgccg ccgttcttgt gacctttatc ggtggtctgt acttcattac
     5881 gcatcacaag aaagaagagt ccgagacgct gcaaagccag aaagtgaccg gaaacggact
     5941 accaccaaaa ccagaagaac gctggcgcta catcaaagag ctggaaagtc gccagccggg
     6001 tgtgcgtgcg cccacagaac cttctgccgg tggtgaagtg aaaacgccgg agcaactgac
     6061 accagaacaa cgtcagcttc ttgaacaaat gcaggctgat atgcgccagc agccaacgca
     6121 gctggttgaa gttccgtgga atgaacagac gccggaacag cgtcagcaaa cgctacagcg
     6181 ccaacgtcag gcgcagcaat tagcggaaca gcaacgcctg gctcagcagt cgcgtacgac
     6241 tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc
     6301 gcgccagtct aaaccggctt ctactcagca gccgtaccag gatctgctgc aaacccctgc
     6361 gcacacgacc gcgcagtcga agccgcagca agctgcgccg gttactcgtg ccgctgacgc
     6421 gccaaaaccg acggcggaga aaaaagacga acgccgctgg atggtgcagt gcggttcgtt
     6481 cagaggcgcg gaacaggcag agacggtacg tgctcagctg gcgttcgaag gctttgactc
     6541 aaaaattacc accaacaatg gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga
     6601 gaacgcagac agcaccctca atcggttgaa gatggcgggt catacaaact gcattcggct
     6661 cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta taattgcatt atgccccgta
     6721 cttttgtacg gggtttgtac tctgtattcg taaccaaggg gtcagctcgt gacaactata
     6781 gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg atggtcaggc cacgttgggc
     6841 aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc
     6901 gcgggctttg cgggcggtac tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa
     6961 ctggaaatgc atcagggcca tctggtcaaa gccgccgttg agctggcaaa agactggcgt
     7021 accgatcgca tgttgcgcaa acttgaagca ctgctggcag tcgcggatga aactgcatcg
     7081 cttatcatca ccggtaacgg tgacgtggtg cagccagaaa acgatcttat tgctatcggc
     7141 tccggcggcc cttacgccca ggctgcggcg cgtgcactgt tagaaaatac tgaacttagt
     7201 gctcgtgaaa ttgctgaaaa ggcgttggat attgcaggcg acatttgcat ctataccaac
     7261 catttccaca ccatcgaaga attaagctac aaagcgtaag gatctcccat gtctgaaatg
     7321 accccacgcg aaatcgtcag cgaactggat aagcacatca tcggccagga caacgccaag
     7381 cgttccgtgg cgattgctct gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg
     7441 cgccatgaag tgaccccgaa aaatatcctg atgatcggcc cgaccggtgt cggtaaaact
     7501 gaaatcgccc gtcgtctggc taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc
     7561 aaattcaccg aagtgggcta cgtcggtaag gaagtggatt ctattattcg cgatctgacc
     7621 gatgccgccg tgaaaatggt acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa
     7681 gaactggcag aagaacgtat tctcgacgtg ctgatcccac ctgctaaaaa caactgggga
     7741 cagaccgaac agcagcagga accgtccgct gctcgtcagg cattccgcaa aaaactgcgt
     7801 gaaggccagc ttgatgacaa agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt
     7861 gaaattatgg ctcctccagg catggaagag atgaccagcc agctgcagtc catgttccag
     7921 aacctgggcg gtcagaagca aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg
     7981 ctgattgaag aagaagcggc gaaactggtg aacccggaag agctgaagca agacgctatc
     8041 gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc
     8101 ggcgagtctt ccggtccgga tgtttctcgt gaaggcgttc agcgagacct gctgccgctg
     8161 gtagaaggtt gcaccgtttc caccaaacac gggatggtca aaactgacca cattctgttt
     8221 atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt
     8281 cgactgccaa tccgcgttga actgcaggca ctgaccacca gcgacttcga gcgtattctg
     8341 accgagccga atgcctctat caccgtgcag tacaaagcac tgatggcgac tgaaggcgta
     8401 aatatcgagt ttaccgactc cggtattaaa cgcatcgcgg aagcggcatg gcaggtgaac
     8461 gaatctaccg aaaacatcgg tgctcgtcgt ttacacactg ttctggagcg tttaatggaa
     8521 gagatttcct acgacgccag cgatttaagc ggtcaaaata tcactattga cgcagattat
     8581 gtgagcaaac atctggatgc gttggtggca gatgaagatc tgagccgttt tatcctataa
     8641 tcgcgttcaa tcattttcat cattgtttga tggggctgaa aggccccatt tttattggcg
     8701 cgtattatga ctgaacaaca aattagccga actcaggcgt ggctggaaag tttacgacct
     8761 aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga cggcgctggc atggtggcaa
     8821 ggtcattttg atccgctggt cgccctgctg gcactgatta ccgccgggct attacagatc
     8881 ctttctaacc tcgccaatga ttacggcgat gcggtaaaag gcagcgataa acctgaccgt
     8941 atcggaccgc tacgcggcat gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg
     9001 ctcattatta ccgtcgtgct catctgtctc tccgggctgg cactggttgc agtggcgtgc
     9061 catacgctgg ccgattttgt cggtttcctg attcttggcg ggttgtcgat cattgccgct
     9121 atcacctaca ccgtgggcaa tcgtccttat ggttatatcg gtctgggtga tatttccgta
     9181 ctggttttct ttggctggtt aagcgtcatg gggagctggt atttacaggc tcatacattg
     9241 attccggcac tgatccttcc ggcgaccgca tgcggcctgc tggcaacggc agtactgaac
     9301 attaataacc tgcgtgatat caatagcgac cgcgaaaacg gcaaaaacac gctggtggtg
     9361 cgcttaggtg aagtgaacgc gcgtcgttat catgcctgcc tgctgatggg ctctctggta
     9421 tgtctggcgc tgtttaatct cttttcgctg catagcctgt ggggctggct gttcctgctg
     9481 gcggcaccgt tgctggtgaa gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg
     9541 atgcgaccga tgctggaacg cactgtcaag ggagcgttac tgactaacct gctgtttgtt
     9601 gtagggatat tcctaagcca gtgggcagca taactgacaa atatcaatta acaattgatg
     9661 attttgccaa cagaccacat agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta
     9721 agcctatgaa atacgatact tccgagcttt gtgacatcta tcaagaagat gttaacgtcg
     9781 tggaaccgct gttctccaac tttggcggac gggcgtcgtt tggcggacaa ataatcacgg
     9841 taaaatgttt cgaggacaac gggttgctgt acgatctgct cgaacagaat ggccgtggtc
     9901 gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg
     9961 cgcgtctggc agtacaaaat gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg
    10021 tagatgacct ggaagagttg gatatcggca tccaggcgat ggcggcaatt ccggttggtg
    10081 ccgctggcga aggcattggc gaaagcgatg tccgcgtcaa ttttggcggt gttaccttct
    10141 tctccggcga ccatctttat gccgacaata ccgggattat tctttcagaa gatccgctgg
    10201 atattgaata agtatctgcc atgcgttaaa aggggaatta aattcccctt ttttatacat
    10261 ggacgtattc atgcagaata ttattctttt atttatatat ttattactta tagccgttaa
    10321 ccatttccgc taca
//
LOCUS       DBFIFS010000156        10314 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.156, whole genome shotgun sequence.
ACCESSION   DBFIFS010000156 DBFIFS010000000
VERSION     DBFIFS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.156"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(296..2869)
                     /gene="clpB"
                     /locus_tag="N7A07_003459"
     CDS             complement(296..2869)
                     /gene="clpB"
                     /locus_tag="N7A07_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU2478057.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="N7A07_003460"
     CDS             complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="N7A07_003460"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU2478058.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="N7A07_003461"
     CDS             complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="N7A07_003461"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU2478059.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4842..5579
                     /gene="bamD"
                     /locus_tag="N7A07_003462"
     CDS             4842..5579
                     /gene="bamD"
                     /locus_tag="N7A07_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU2478060.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5850..6191
                     /gene="raiA"
                     /locus_tag="N7A07_003463"
     CDS             5850..6191
                     /gene="raiA"
                     /locus_tag="N7A07_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU2478061.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6294..6419
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6295..6342
                     /gene="pheL"
                     /locus_tag="N7A07_003464"
     CDS             6295..6342
                     /gene="pheL"
                     /locus_tag="N7A07_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU2478062.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6441..7601
                     /gene="pheA"
                     /locus_tag="N7A07_003465"
     CDS             6441..7601
                     /gene="pheA"
                     /locus_tag="N7A07_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU2478063.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="N7A07_003466"
     CDS             complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="N7A07_003466"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU2478064.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="N7A07_003467"
     CDS             complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="N7A07_003467"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU2478065.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10056..>10314
                     /locus_tag="N7A07_003468"
     CDS             10056..>10314
                     /locus_tag="N7A07_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU2478066.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLS"
BASE COUNT         2615 a         2734 c         2425 g         2540 t
ORIGIN      
        1 ccgcttcgct ttttctcagc ggcgcggggt gtgcatgata cgctttcccg ctacagagtc
       61 aagcatttat ttttgctttc tctgccggaa ttctcaggag aaccccgctg acccggcggc
      121 gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca
      181 agcgaaaaat ataaaaactt tatcgttcgc tcatttttca ggcaaaacat cttaaatata
      241 gtcttttccg tctaacttat agacaaaaac gagccccgaa gggctcgttt tatcattact
      301 ggacggcgac aatccggtct tcattaactt ccaggcgaat aactttaccc ggaaccaatt
      361 caccagacag tatttgctgt gccaacgggt tttcgatctg ctgctggata gcacgtttca
      421 aaggacgtgc accatagact ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt
      481 cagaaatgtg gatttcataa ccacgttctt ccagacgttt gtacagacgt ttcaactgaa
      541 tctgcgcaat cgaggcaatg tgctgttcgc ccagcggatg gaagaccacc acttcatcga
      601 tacggttaat gaactccgga cggaagttat ggcttaccac accgagcacc agctctttca
      661 tgtgcgcata atccagttca ccgaagcgtt cctgaatcag atcggaaccg aggttagagg
      721 tcataatgac gaccgtatta cggaagtcga ccgttctccc ttgcccgtca gtcagacgcc
      781 catcatccag tacctgcaac agaatgttga agacatccgg atgcgctttt tccacttcat
      841 ccagcaggat gacggaatac ggacgacgac gcaccgcttc ggtcaggtag ccaccttctt
      901 cataaccgac atatcccgga ggcgcaccaa ccagacgaga caccgagtgt ttctccataa
      961 actcggacat atcgatacgg accatcgcct cgtcgctatc aaacataaag ttcgccagcg
     1021 ccttacaaag ctctgttttc cccacaccag ttgggccgag gaacaggaat gaaccaatcg
     1081 ggcgatttgg atccgccagc cccgcacggc tacgacgaat agcgttagat accgcatcaa
     1141 ccgcttcgtt ctgaccaatt acgcgatggt gcagttcttg ctccatacgc agcagttttt
     1201 cgcgctcgct ttccatcatg cgagaaaccg gaatccccgt ccaacgcgcc agcacttcag
     1261 caatttcggc gtcggtcact ttattacgca acagacgcat agttttgcct tcgagctgcg
     1321 ttgcggcttc cagttgcttt tccagttccg ggattttgcc gtattgcagt tcagacatcc
     1381 gcgccaagtc acccacacgg cgagcctgtt caatagcgat tttcgcctgt tccagttccg
     1441 ctttaatggt ctgcgtacca gaaagcgatg ccttctctgc tttccactct tcttctaact
     1501 cggagtactg acgttctttg tcgctcagtt cttcgttgag catatccaga cgttttttac
     1561 tggcttcatc agactctttc attaacgcct gttgttccag tttgagctgg atgatacggc
     1621 gatcgagtcg gtcgagttct tctggttttg agtcaatctg catacgaatg ctggatgctg
     1681 cttcatcgat caggtcgatg gctttatccg gcagctgacg gtcagcaatg tagcgatgag
     1741 acaacgtcgc cgctgcaaca attgccgggt cagtaatttg cacatggtgg tgcaattcgt
     1801 aacgttcttt caggccacgc agaatcgcaa tggtatcttc aacagaaggc tcggcaacaa
     1861 acactttctg gaaacgacgt tccagcgcag catctttttc gatgtactgg cgatattcgt
     1921 caagcgtcgt ggcacctacg cagtgcaatt caccacgcgc cagcgccggt ttcagcatgt
     1981 ttccggcgtc cattgcgcca tcggctttac ccgcgccgac catggtatgt aattcgtcga
     2041 taaacaggat gacgttgcct tcctgtttgg caagatcgtt aagcacgcct tttaaacgtt
     2101 cttcaaactc accgcgatat ttcgccccag ccaccagcgc gcccatatcc agcgccagta
     2161 cccggcggcc tttcaaccct tccggcactt cgccgttgat aatacgctgc gccagacctt
     2221 caacgatggc agttttaccg acgccgggtt caccaatcag taccgggtta tttttagtac
     2281 gacgttgcag cacctgaatg gtacggcgaa tttcttcatc acgaccaatc accggatcga
     2341 gtttgccctg ttcggctcgt tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt
     2401 ggtcttcagc accttgatcg ttcacgcttt cacctccacg catttgttca atcgcttgag
     2461 taatgttggc ggtggtcgcc cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact
     2521 caagtgccgc cagaacgaac agttctgacg agataaagtt atcaccacgt ttttgcgcca
     2581 gcttgtcgca aagattaaga acgcgcacca gatcctgtga tggctggaca tcaccaccag
     2641 taccttcaac ctgcggtaaa cgatttaatg cctgattgat atctgtgcgc aactggccag
     2701 catttatgcc agcggatgtt aataaaggac taaccgaacc cccttcctga ttcagcaggg
     2761 cgctcattaa atgaagtggt tcgataaatt ggttgtcgtg cccaagtgca agtgattggg
     2821 catcggcaag agcaagctgg aatttattag taagacgatc cagacgcata actcctccca
     2881 taacggatca aagttgctac tggagattaa atgaggtcat ccctcaatta ttcaaggtta
     2941 ttgaccagat taatgtgaaa agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt
     3001 atatcagcca aatgaaactt gccatacgac cggtggtctt gtcgcgacga taagagaaga
     3061 aagtctcatt ttccgtatat gtacaacggt cgccgccgaa aatttgctca acacccacgt
     3121 tcgccagacg ctgccgggca agctgataaa tgtccgccag atacttatca ccatgctgaa
     3181 tgaaagctgt actcgcttcg gcgtctactg ccataaacgc ctcgcgaacc tccgccccca
     3241 cttcaaacgc gcgtgggcca attgccggcc ctaaccaggc gagaatattt tctggattat
     3301 cagcaaaaca ggaaactgtc tcttccagca cgcctgcgca cagtccacgc cagccagcat
     3361 gagcggcggc gacttccgtt cccgctcgat tgcaaaacag cacagggagg cagtcggcag
     3421 tcatcactgc gcaaaccctg ccgggcgtat tgctataaga ggcatctgct cgttttgagg
     3481 cataaggttc gccagtaaga ttaagcacat ctttgccgtg tacctgctca agccagaccg
     3541 gtttagaagg caaattgccc gcagcaaaaa gtcgcttgcg attctcctca acgtgatccg
     3601 ggttatcgcc acaatgggca ccgaggttga gcgagtcata cgggggcaag ctcacgccgc
     3661 cgatacgagt agagctacag gccgcaacac cttttggcag cggccactgc gggacaatca
     3721 gcttactcat aaccagtcca cttcatcttt atgctcttcg aaatcggcgc gcatcacctc
     3781 aatcagctcc accatatctt gtggaatagg cgcatgccat tccatttcga taccggagat
     3841 cgggtgataa agacgcagca tggttgcatg aagcgcctgg cggtcaaatt tacgcagcgt
     3901 ggagataaac gcttccgaag caccttttgg cgggcgcgga cggccaccat aaaccggatc
     3961 gcccaccagc ggatgagtga tatgggccat atgcacgcgg atctggtgcg tacgtccagt
     4021 ttccagacgc aaccgcagac gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt
     4081 caccgccggt ttgcccatcg gatgcaccgc catgtgagta cgtttggtcg ggtggcggct
     4141 gattggttca tctactgtac cgccagcggt catatgacca attgccaccg cttcatactc
     4201 acgagtaatt tcacgccttt gcaaagattc gactaaacgc gtctgagccg gaacggtttt
     4261 tgccacaacc atcaggccag tggtgtcttt atccagacga tggacgatgc ctgcacgcgg
     4321 tacatcagca atgggcggat agtaatgaag taacgcattc agtaccgtgc catccgggtt
     4381 acccgcgcca ggatgtacca ccagatcgcg cggtttatta atgacgatga tatcttcatc
     4441 ttcatagacg atatccagcg ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc
     4501 agcgttgatg gcaacctgct cgccacccaa tactttttct ttcggcttat cacaaagttt
     4561 gccgttaacc agcacgcgct ggtcgaggat ccattctttt attcgcgaac gtgaataatc
     4621 cgggaacatt tcggccaaag cctgatctaa gcgttgaccg agttggtttt cggacaccgt
     4681 tgcagtgagc tgtactcgtt gtgccatata tactgcttct tcgtttatcg ttggggtttt
     4741 acggctttgc cgtttaatat agtgtgctat tgtagctggt cttaaccggg agcaggaaca
     4801 gagaatctcc cgtattacat tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc
     4861 tggtggcagc cgccacacta agcctgtttt tggcgggttg ctcggggtca aaggaagaag
     4921 tacctgataa tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta
     4981 actggagaca ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt
     5041 attcgcagca ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt
     5101 tagcgcaggc tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt
     5161 atgtcatgta catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt
     5221 tctttggcgt tgaccgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact
     5281 tttccaaact ggtgcgcggc tatccaaaca gtcagtacac caccgatgcc accaaacgtc
     5341 tggtattcct gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag
     5401 aacgtggcgc atgggttgcc gtcgttaacc gcgtagaagg catgttgcgc gactacccgg
     5461 atacccaggc tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga
     5521 tgaatgcgca agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac
     5581 agaaacctga aacacaaaac ggcagccctt gagctgccgt ttttttattc tgtcagttgt
     5641 gaaactgaag cgatttagtc actatcgatc tcatcaaata tggctcgctt tgagatattc
     5701 ctcaagtaaa aaaacacctc ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag
     5761 tgacaaaatt atgagatttc catcacacat tttgacatca ggaacggtat gctgaattca
     5821 ccaagacggg aagacaagag gtaaaattta tgacaatgaa cattaccagc aaacaaatgg
     5881 aaattactcc ggccatccgc caacatgtcg cagaccgtct cgccaaactg gaaaaatggc
     5941 aaacacatct gattaatcca catatcattc tgtccaaaga gccacaaggg tttgttgctg
     6001 acgccacaat caatacacct aacggcgttc tggttgccag tggtaaacat gaagatatgt
     6061 acaccgcaat taacgaattg atcaacaagc tggaacggca gctcaataaa ctgcagcaca
     6121 aaggcgaagc acgtcgtgcc gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg
     6181 aagaagagta gtcctttata ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt
     6241 tgacagcgtg aaaacagtac gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa
     6301 cacacaccgt ttttcttcgc attctttttt accttcccct gaatgggagg cgtttcgtcg
     6361 tgtgaaacag aatgcgaaga agaacaataa ggcctcccaa atcggggggc cttttttatt
     6421 gataacaaaa aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagagaaaa
     6481 tcagcgcgct ggatgaaaaa ttattagcat tactggcaga gcggcgcgaa ctggccgtcg
     6541 aggtgggaaa agccaaactg ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg
     6601 atttactgga aagattaatt acgctcggta aagcgcacca tctggacgcc cattacatta
     6661 ctcgcctgtt ccagctcatc attgaagatt ccgtattaac tcagcaggct ttgctccaac
     6721 aacatctcaa taaaattaat ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt
     6781 cttattccca tcttgcggcg cgccagtatg ctgcccgtca ctttgagcaa ttcattgaaa
     6841 gtggctgcgc caaatttgcc gatattttta atcaggtgga aaccggccag gccgactatg
     6901 ccgtcgtacc gattgaaaat accagctccg gtgccataaa tgacgtttac gatctgctgc
     6961 aacataccag cttgtcgatt gttggcgaga tgacgttaac tatcgaccat tgtttgttag
     7021 tctccggcac gactgattta tccaccatca atacggtcta cagccatccg cagccattcc
     7081 agcaatgcag caaattcctt aatcgttatc cgcactggaa gattgaatat accgaaagta
     7141 cgtctgcggc aatggaaaag gttgcacagg caaaatcacc gcatgttgct gcgttaggaa
     7201 gcgaagctgg cggcactttg tacggtttgc aggtactgga gcggattgaa gcgaatcagc
     7261 gacaaaactt cacccgattt gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg
     7321 ttccggcgaa aacgacgttg ttaatggcga ccgggcaaca agccggtgcg ctggttgaag
     7381 cgttgctggt actgcgcaac cacaatctga ttatgacccg tctggaatca cgcccgattc
     7441 acggtaatcc atgggaagag atgttttatc tggatattca ggccaatctt gaatcagcgg
     7501 aaatgcaaaa agcattgaaa gagttagggg aaatcacccg ttcaatgaag gtattgggct
     7561 gttaccctag tgagaacgta gtgcctgttg atccaacctg atgaaaaggt gccggatgat
     7621 gtgaatcatc cggcactgga ttattactgg cgattgtcat tcgcctgacg caataacacg
     7681 cggctttcac tctgaaaacg ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa
     7741 ctgtcaataa acgcctgctt atcgccctgc tccagcaact caatcgcctc gccgaaacgc
     7801 ttatagtaac gtttgattaa cgccagatta cgctctgacg acataataat gtcggcataa
     7861 agctgcggat cctgagcgaa cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc
     7921 gaagagagcg ccagaagttg ctcaagctga acattttctt ctgccaggtg cagcccgtaa
     7981 gcaaaagtag caaagtggcg cagtgcctga ataaacgcca tattctgatc gtgctcgacg
     8041 gcgctaatac gatgcaaccg agcgccccag acctgaattt gctccagaaa ccattggtat
     8101 gcttccggtt tacgtccatc acaccagacc acaacttgct ttgccaggct accgctgtcc
     8161 ggaccgaaca ttgggtgtaa ccccagtacc gggccatcgt gcgccgccag catggcctgt
     8221 aatggtccat ttttcactga tgccagatca accagaatac aatctttcgg taaaggcggt
     8281 aatttgccaa taacttgctc agtaacgtgg attggcacac taacaatcac cattccggca
     8341 tcggcaacaa tatcagccgc tcgatcccag tcatgttgct ccagaatccg cacctgataa
     8401 cccgagagtg tcagcatctt ctcgaacaga cgtcccatct gaccgccgcc gccaacgata
     8461 actaccgggc gtaacgcagg acaaagcgtt ttaaatcctt tgtcgttttc actggagtaa
     8521 gattcacgca tcacccgacg caaaacatcc tcaatcagat ctggcggtac acccagagct
     8581 tccgcctctg cacgacgcga ggccaacata gatgcctctc gctccggaac ataaataggc
     8641 agtccaaagc ggcttttcac ctcgcccact tcagcaacca gttccagacg cttcgctaat
     8701 aaattcagca gcgctttatc gacttcatca atttgatcgc gtaatgcggt caattcagca
     8761 accataataa acctcttaag ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat
     8821 ttcacgcagc aaggcatcgg tcatttccca gctaatgcag gcatcggtta cggatacacc
     8881 gtatttcatt tcactgcgcg gttgctcgga agactgattg ccctcgtgga tattactttc
     8941 gatcatcaga ccaataattg agcgattgcc atctttgatt tgagcaacca cggattctgc
     9001 caccgcaggc tgacggcgat aatctttatt ggaattaccg tggctgcaat ctaccatcag
     9061 agacgggcgc agtcccgcct gttccatctc tttttcacat tgcgcaacat ccgcagggct
     9121 atagttcggc gctttaccac cgcgcaggat gacatgcccg tccggattcc cctgagtttg
     9181 tagcaacgca acctgccctg cctggttaat gccaacaaaa cggtgcggct gggcggcggc
     9241 gcgcatagcg ttaattgctg ttgccagact gccgtcggtg ccgtttttaa agccaaccgg
     9301 catggaaagc ccggaggcca tttcacggtg agtttgcgat tccgttgtac gagcaccaat
     9361 tgctgaccag ctaaacagat cgcccaggta ttgcgggcta ttcggatcta acgcttccgt
     9421 cgccagtggc agtcccatat tcaccagctc aagcagcaat ttacgcgcga tctgcagccc
     9481 ggcttctaca tcaaaagagc catccatatg gggatcgtta attaaccctt tccagccgac
     9541 agtggtacgg ggtttttcaa aatagacgcg cattaccaga tagaggctat cgctgacctc
     9601 tgcggcaagg gctttaaatc gacgagcata ttccagagca gtttccggat catgaatgga
     9661 acaagggcca catactacca gcagacgagg atcgcgcccg gcgataatat ctgaaatggt
     9721 tttacgcgag tcagcaatct gggcttcttg ttgcaggctt aatggaaaag cggccttcag
     9781 ttgtgccgga gtcattaaaa cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt
     9841 ttgcatgatg gcgatcctgt ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac
     9901 gtaacgataa cacataaagt aaagttttca atccatattt cgtacatttt tctttacata
     9961 agcaatttat tcgcgctttc aacccttgat tctgtataga taaatttaca ccacctttga
    10021 aaacaatccg ctatgctttg aaaaaggaga aagaaatgat gaaaaagttt atcgccccct
    10081 tgttggcttt actggttagc ggatgccaga ttgatcctta tactcacgcg ccaaccttga
    10141 ccagcaccga ctggtatgat gttggtatgg aagatgcgat atcgggcagc gccataaaag
    10201 atgacgatgc atttagcgat tcacaggcgg atcgcggtct ataccttaaa ggatatgccg
    10261 aaggacaaaa gaaaacttgc cagaccgatt ttacttatgc ccgaggactt tccg
//
LOCUS       DBFIFS010000157        10152 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.157, whole genome shotgun sequence.
ACCESSION   DBFIFS010000157 DBFIFS010000000
VERSION     DBFIFS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.157"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(56..1246)
                     /gene="bcr"
                     /locus_tag="N7A07_003469"
     CDS             complement(56..1246)
                     /gene="bcr"
                     /locus_tag="N7A07_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU2478067.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1274..1969)
                     /gene="rsuA"
                     /locus_tag="N7A07_003470"
     CDS             complement(1274..1969)
                     /gene="rsuA"
                     /locus_tag="N7A07_003470"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU2478068.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2118..3878
                     /locus_tag="N7A07_003471"
     CDS             2118..3878
                     /locus_tag="N7A07_003471"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2478069.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4003..4287
                     /gene="rplY"
                     /locus_tag="N7A07_003472"
     CDS             4003..4287
                     /gene="rplY"
                     /locus_tag="N7A07_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU2478070.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4426..5433)
                     /gene="yejK"
                     /locus_tag="N7A07_003473"
     CDS             complement(4426..5433)
                     /gene="yejK"
                     /locus_tag="N7A07_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU2478071.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5615..5842
                     /locus_tag="N7A07_003474"
     CDS             5615..5842
                     /locus_tag="N7A07_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU2478072.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5862..7622
                     /gene="yejM"
                     /locus_tag="N7A07_003475"
     CDS             5862..7622
                     /gene="yejM"
                     /locus_tag="N7A07_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU2478073.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7697..7773
                     /locus_tag="N7A07_003476"
     tRNA            7697..7773
                     /locus_tag="N7A07_003476"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7731..7733,aa:Pro,seq:ggg)
     gene            complement(7874..>10152)
                     /gene="yejO"
                     /locus_tag="N7A07_003477"
     CDS             complement(7874..>10152)
                     /gene="yejO"
                     /locus_tag="N7A07_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGU2478074.1"
                     /translation="GGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIE
                     KGGLLEVKEGGLAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGG
                     SLRVEENDFAYNTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQF
                     SIKDGVSKNYELDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGR
                     SDQNGSITYSSKAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAML
                     VGKVVVSEGASLRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGAN
                     VIMMDESVTRSSVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDF
                     KIFVTDTGASPAAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLA
                     ENRAQITPSTTDVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNV
                     TTDAGAGFEQTLTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTL
                     GAYAGWEHQNGAYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVD
                     GLWSVRPYLAFTGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLE
                     PWLKAAVRQEYADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGN
                     GAGVESPWNTQAGVVWTF"
BASE COUNT         2525 a         2761 c         2430 g         2436 t
ORIGIN      
        1 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
       61 gttttttcgg ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
      121 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
      181 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
      241 gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa
      301 acgccgcaac gccaaccacc agcgaccaaa atcccagacc cagcagcgca ctgatgacca
      361 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
      421 tacggcggac gaagcggcta ttaaagatgg tcatcacgaa caggaaaaca atgtttagtg
      481 caaagtaata accaaagttt tccggcgcga cgtggttaat ttcaatgtag acaaacggtc
      541 cggcgcttaa gaacgagaac atcccggcaa agctgaaacc actggccagc atataactca
      601 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
      661 gctggcgacg ctccggtggt aaggtttctt tgatcagaaa gaagatcatt gccgaagcca
      721 gaatcgccgc cacggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc
      781 agccgccaac tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca
      841 tcatccgcga gaactcttct ttcgggtaga tatcacgcat caaagcgttg ataaccacgc
      901 tggccgcagc cgccgccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt
      961 gtgccaacgc acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg
     1021 gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca
     1081 gaatataagt actgagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa
     1141 ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc
     1201 caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa
     1261 agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac
     1321 cgggggctaa atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca
     1381 catggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg
     1441 tcagacgaac ctgcgttggg gtaatcactt caagcaccgc tggcttagtg agatcttttt
     1501 cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acgggtgatt
     1561 ccagcgtcac cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc
     1621 actgaccatc atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg
     1681 cgtgcagttt ccacgccact ggttcatcta gaaaatagag caccgttggg tgatcagggt
     1741 cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt
     1801 gctgcgccag cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg
     1861 cattacggac gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa
     1921 tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt
     1981 tgcctgtaaa aacggaggtc gggcattgac ccgaaaatct gaactgttgt ctgcccagta
     2041 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
     2101 tctacctacg cgagaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
     2161 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggtg
     2221 caggtaaaag cctggtgata gcggaacttg cgcggctggc tcgtggtcgc gtgctggtgc
     2281 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
     2341 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
     2401 gcagcgtgca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
     2461 ttgtcgatga atgtcaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
     2521 ctcacctgac aaaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
     2581 gactgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
     2641 aagccctttt ccgtgactgc atttatgaac tgccgctgcg ttatatgatt aaacacggct
     2701 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
     2761 aggcacagag taacgggcta ttcagcgaag ccgatctcaa ccgcgagcta aaaaaacaac
     2821 aacgtattac cccgcacatc atcagccaga ttatggagtt tgctgcaacg cgcaaagggg
     2881 tgatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctacccgccg
     2941 aagatgctgc actgattact ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaag
     3001 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc ggtactgacc accggatttg
     3061 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
     3121 aacaaattgt cgggcgcggt ctgcgtctcg ctcctggcaa gactgattgc ttaattcttg
     3181 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
     3241 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
     3301 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
     3361 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
     3421 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
     3481 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
     3541 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
     3601 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
     3661 agcgaactgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
     3721 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttattgcga cacccggatt
     3781 ttgtcgttgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
     3841 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
     3901 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
     3961 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
     4021 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
     4081 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
     4141 gtcatgaaca ttcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
     4201 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
     4261 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
     4321 cggggttttt tgtatctgca gattatgcct gattacgata ttgctatttt ttgcaggcca
     4381 gataaggcgt tcacgccgca tctggcattc ataacacaaa accgattaat tgccgccgga
     4441 cgtccggcgc tgcaattgat cgcgcaaatt cggcggcgtg cctttaatag tcagggtatc
     4501 ggtcgccgga tcccagaaaa tccgttcgcc caacagcatc gcatcaaaat taatagtcaa
     4561 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
     4621 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgctgac
     4681 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
     4741 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
     4801 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
     4861 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
     4921 tttcaggaaa gtgagatagc gggtcgactc tggattggtt tcccattcgg ttaaatcgat
     4981 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
     5041 ttcgttaacg cgcatactgc tcaggttgct caggactgcc accagcagat actccaccgc
     5101 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
     5161 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcaccctg
     5221 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
     5281 atttttggcg ctatagaccc gatgcagttc tgctaccatc tcaacgacgg tttctgtcgg
     5341 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
     5401 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
     5461 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggcg
     5521 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
     5581 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtga
     5641 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
     5701 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
     5761 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
     5821 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
     5881 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
     5941 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgaccacgc
     6001 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
     6061 ccacctactt gctgattctc ttcccgctga cctttatcgt cggctcccag aggctgatga
     6121 ggtttttgtc cgtcattctg gcaacggcgg gaatgacgct attactgatc gatagcgaag
     6181 tctttactcg tttccatctc catcttaatc ccatcgtctg gcaactggtt attaacccag
     6241 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
     6301 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
     6361 ggcgcttcgc gcgtccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
     6421 tgtatatctg ggccgatgcc aacttctatc gccctatcac catgcagcgc gctaacctgc
     6481 cgctttcgta cccgatgacg gcgcgacgtt ttcttgagaa gcatggcctg cttgatgcgc
     6541 aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc gttcagtatc
     6601 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtcttg ttgattactg
     6661 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
     6721 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
     6781 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
     6841 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
     6901 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
     6961 tacgcaccca atccgacgag cagaccgcca cgcagtggat caactggctg ggacgctacg
     7021 cacaagaaga taaccgctgg ttctcgtggg tctctttcaa tggtactaac attgacgaca
     7081 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
     7141 tcaaccgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
     7201 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
     7261 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
     7321 cgctgactga tcataccgat ctgatgacga cgctgatgca acgcctgcta catgtcagca
     7381 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
     7441 gggttaccgc agcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
     7501 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
     7561 aaccacagtt aagtttgtta ttgcaagtac tgacagacga gaagcgtttt atcgctaact
     7621 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
     7681 attaacaacc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg
     7741 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caaaaaaacc aaccgtaaag
     7801 gttggttttt ttatatctgc atttaattcg ataaacagac cgcgacacat cagggcctgt
     7861 ttattgtctg ttatcagaac gtccagacca cacccgcctg ggtattccac ggcgattcta
     7921 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
     7981 accttatccc agcctgataa acgccacggg ttccagccac atcattatta aatttgccat
     8041 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
     8101 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
     8161 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
     8221 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
     8281 caacccaacg gaacccgctc tcgacatgag cacccgcgcc gttactattg taatcgccaa
     8341 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
     8401 ccactccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
     8461 agctatcgac attgcctttg ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
     8521 agaacaagcc gcgaattgtg ctgctttctt cacgggagaa acggctatcg ataccgagcg
     8581 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
     8641 tgttaattgc cgaactccac atcgccgtat cgtaactaac gccttttacg ctaccaagac
     8701 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggcc gccatattca
     8761 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgccagactc cagctatggt
     8821 taccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
     8881 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
     8941 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
     9001 cgttgagctg atcgctctga tgattcgcca tatcggtacg catataaaaa ttgccgtttc
     9061 ccgacagggt attggtggtc aacgtagtga aattttccgc acttgccgtc acagatgaac
     9121 gagtcactga ctcatccatc ataatcacat ttgcgccaga catcgcaagg ttggctaagt
     9181 taaggcgggt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
     9241 cgagcgaaac atccgctttg ctggtatcca cggcaccatg cgttcttaaa gaagcgccct
     9301 cagaaaccac taccttaccc accaacattg cgggtgcata atttatttgc ggcttcataa
     9361 cctcaagaat gccatcattt cctctgactg acacgttaac cattgtgcca gcccagacgt
     9421 tagcgcggcc attgttgata accatatttt cagagatggc tttagaggaa tacgttatac
     9481 ttccattctg atctgatcga ccgagatcat ataccgcatt tgcctgcact ctcgtaccag
     9541 tatcctttcc cagcgattgc atagtggcat gctcatcgag gatagtgtca atggcctgcg
     9601 tgtcctccat aacaataagc ccggaaccat catccagttc ataatttttt gacacaccat
     9661 cttttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
     9721 caattaattt tccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
     9781 ccagtaagcc gccactatct acagtagtat tataagcgaa gtcattttct tcaactcgca
     9841 aacttccgcc gttttccaac agcatattgt tagcaatacc attcttgatt tcgaactgac
     9901 cgagacggtt tgttccgaat acctccatga cccgcgtgct tgctttaata gcaccgcctg
     9961 ctttctgatc taccgcaatc gctaatcccc cctctttaac ctccagtaat cctccctttt
    10021 cgatagcggc aaggacggta atcccatctt tttcaacgat taagcgcccg cctttaatcg
    10081 tgggatcatt cgctgcgcca taaacctgta gcacaccatt attgagcgta gtctggtttg
    10141 ccgcgccacc ca
//
LOCUS       DBFIFS010000158        10074 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.158, whole genome shotgun sequence.
ACCESSION   DBFIFS010000158 DBFIFS010000000
VERSION     DBFIFS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.158"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            203..475
                     /gene="ynjH"
                     /locus_tag="N7A07_003478"
     CDS             203..475
                     /gene="ynjH"
                     /locus_tag="N7A07_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU2478075.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(441..848)
                     /gene="nudG"
                     /locus_tag="N7A07_003479"
     CDS             complement(441..848)
                     /gene="nudG"
                     /locus_tag="N7A07_003479"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU2478076.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            935..1555
                     /gene="ynjF"
                     /locus_tag="N7A07_003480"
     CDS             935..1555
                     /gene="ynjF"
                     /locus_tag="N7A07_003480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU2478077.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1564..2871)
                     /gene="ynjE"
                     /locus_tag="N7A07_003481"
     CDS             complement(1564..2871)
                     /gene="ynjE"
                     /locus_tag="N7A07_003481"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU2478078.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(2938..3591)
                     /gene="ynjD"
                     /locus_tag="N7A07_003482"
     CDS             complement(2938..3591)
                     /gene="ynjD"
                     /locus_tag="N7A07_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU2478079.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNTRRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3591..5126)
                     /gene="ynjC"
                     /locus_tag="N7A07_003483"
     CDS             complement(3591..5126)
                     /gene="ynjC"
                     /locus_tag="N7A07_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU2478080.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(5099..6265)
                     /gene="ynjB"
                     /locus_tag="N7A07_003484"
     CDS             complement(5099..6265)
                     /gene="ynjB"
                     /locus_tag="N7A07_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2478081.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(6275..6823)
                     /gene="ynjA"
                     /locus_tag="N7A07_003485"
     CDS             complement(6275..6823)
                     /gene="ynjA"
                     /locus_tag="N7A07_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU2478082.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(6823..7530)
                     /gene="ydjZ"
                     /locus_tag="N7A07_003486"
     CDS             complement(6823..7530)
                     /gene="ydjZ"
                     /locus_tag="N7A07_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGU2478083.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(7545..8222)
                     /gene="ydjY"
                     /locus_tag="N7A07_003487"
     CDS             complement(7545..8222)
                     /gene="ydjY"
                     /locus_tag="N7A07_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU2478084.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            complement(8227..8937)
                     /gene="ydjX"
                     /locus_tag="N7A07_003488"
     CDS             complement(8227..8937)
                     /gene="ydjX"
                     /locus_tag="N7A07_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU2478085.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            complement(9104..9910)
                     /gene="xthA"
                     /locus_tag="N7A07_003489"
     CDS             complement(9104..9910)
                     /gene="xthA"
                     /locus_tag="N7A07_003489"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU2478086.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2466 a         2662 c         2617 g         2329 t
ORIGIN      
        1 gcttatgttg tttatgtcat gtgcttttgc agttttcgac tataccatca agaatgtgac
       61 ggtaagcaag aaaaaaatga cccaggaaag caaattaata acgagagtaa tctcataacc
      121 aaatgcgata actcagacgc taaagttttg ccacccggct accgttatca gtagcagaga
      181 caataacgtt aaggagttcc ctgtgagtcg agcattgttc gccgtagtat tagcatttcc
      241 attaattgca ctggctaacc cgcattatgg tccggacgtg gaggtaaacg taccgccaga
      301 agtggtcagt tcaggcgggc agtcagcgca gccctgtact cagtgctgcg tttatcagga
      361 taaaaattat tctgaagggg cggtaattaa agccgaagga atactgctgc aatgccagcg
      421 ggatgacaaa acccttagca ctaatccgct ggtctggcgg cgcgtaaagc cataaacgcc
      481 tctaataatg gaatgtcagc aggagccagc ggatattgca gcgcctcttc aggtgagcac
      541 cagaccagcg cctgatgttc atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag
      601 gcatgaagat ggataatccg gcccgaaact tctcgctgat ggctggcaac atactcaccc
      661 acagttgctt cgatgcccaa ttcttcgttt aactcacgca ccagcgcctg ctgctggctt
      721 tcatccggct cgactttacc accggcaaac tcccataatc ccgcctgatc gctctgggcg
      781 gggcgttgcg cgagtaaaat tttgccatca cgttcaatga tggcggcaac aacttcaatc
      841 attttcataa attacacacg ggcagataag cgaaaaatgc cataatatcc gcactgttgc
      901 tcaatcagaa aggctttata tttaaggagt gcctgtgcta gaccgccatc ttcatccccg
      961 gattaaaccg ttgttgcatc agtgtgtgcg ggttcttgat aaaccgggca ttacgccaga
     1021 tggtttaaca ttagtgggat ttgccatcgg tgtgctggca ctgccttttc tggcgctggg
     1081 ctggtatctg gcggcgcttg tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg
     1141 tgcgctggcg cggcgcagag ggcttaccga cgcgggcggc tttcttgata tttctctcga
     1201 ttttcttttt tacgcgctgg taccttttgg gtttatcctc gccgcaccgc agcaaaacgc
     1261 cctggcaggc ggctggctgc tgtttgcgtt tatcggcacg ggtagcagtt ttctcgcttt
     1321 tgctgcgctg gcggcgaaat atcagattga taatcccggt tatgcgcata aatcgtttta
     1381 ttatctgggg ggattaaccg aaggcacaga aacgatctta ctgtttgtgc tgggatgttt
     1441 attccctgca tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac
     1501 ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc agtaattgca
     1561 atgttatttg ctgctgtccg ggccgcgttc accggttgct accggatttt ttggatcgct
     1621 gctccattcg taccagccgc cgtcgtaaac ggagacgtta ttccagccca ttgctcgtgc
     1681 gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc
     1741 tggtttgata ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc
     1801 atccggatta tggaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc
     1861 tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc
     1921 tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg
     1981 ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat ccggttccgc
     2041 tttcactttc ggtggcgtac cgcgctcaac gggcagaccc gcgtcggacc aggtttgcca
     2101 gccgccatcc agcaggcgca catctttcac gccggcataa agcataatct gcgccacacg
     2161 cgctgcagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg
     2221 ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc
     2281 cacttcgttg gtatcgatgt agtcagcacc gggaatatgg ctgataaggt aaagcttagg
     2341 agcgcccctg gccgcttcaa tgactttcca gtcaccggca ggtttcgccg taacctcttt
     2401 accttgttgc aggtcgtgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt
     2461 ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc
     2521 tttttgcagt cgcgttttga cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg
     2581 ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt
     2641 gtcaagccag ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa
     2701 ggtttgtggc cagccgttat aaaacgcgct ggagcgagta tctatcgctt tgccattttg
     2761 ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca
     2821 agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc
     2881 cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta
     2941 tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg
     3001 aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagttcgc gaacttcgct
     3061 gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc
     3121 cagtagcaac gcttttggct gagcgagaag ggcgcgaagc agagcaacgc gcgctcgctg
     3181 accgccagac aaagtagcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag
     3241 tgcatcattc acggcattac gtcgggtatt ccccttaagt gtcgccggta gcgccagcag
     3301 taaattttgc ccgacactga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat
     3361 ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt
     3421 acaagaaaac tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca
     3481 gccagacggc cccattaacg tgacaatgtc acctttatcc accgtaaagt taacgtttgt
     3541 cagcaagcaa ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggagt
     3601 ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc
     3661 ggtaatagca gttgccataa agccagggcg gcgagaatac cgttgctgcc gccgctgctt
     3721 aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc
     3781 atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcgt agggcgtagc
     3841 ataagcggac atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgtgca
     3901 atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac
     3961 ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc catgttcagc
     4021 catagcgcca gcgtgtactg gcccgccacc agcggcagtg ccggtaataa aatcggcaac
     4081 catagccaca actggcgacg ctgtggcccc cattccagcc acagcagtaa caggagcaaa
     4141 gcgatgaatg tcgccaccag ccccattgtc aggctgttga tgagcgcttc actattgatc
     4201 gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacactggt caagggtaaa
     4261 aaactcgcca gcgtaatgcc tggcaataaa ggcgtggcag gcttacgaac gccatctacg
     4321 cggggaatag tacgccgcca ggtacaccat agcagatagc ccagcaaaac gtaggcggcg
     4381 agtaacaaca tcagcagcag gctggcgagt gcgcctttcg tttgttgatc ggcgtcgccc
     4441 tgagttaacc actgccagct aattactgcc agcgtcggtg gattacctgg cccaagaata
     4501 atcgccacat ctacgaccga cagcgaccag gcaacaatcg ccagcatcgc cattgccagc
     4561 gcaggcgcga cggagggcaa cagcagccag ttcaggcatt gccagcggct gtagcccagc
     4621 gaatccagca cgatgacttg ctgtaaaagc cgtttttcac tcaataccgc tgctaagatc
     4681 cacagcagaa atgcgctttc tttcaccgcc agcgtgaagc ccagcccaat acccaatttg
     4741 tccattggcg gagtgaaata cgggaaatag tcataaagca gccctccgtc agcaaagagc
     4801 agaagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg cagacgggtg
     4861 cacatacgct gccatttcgg cccaggccac agcgccacaa tcaccagtag ggcaatcaac
     4921 aacgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc ctgcggtaac
     4981 tgtggatcgg caaataacgc ctgccagtgt gtcaacgaca aagcaggcga tatcagcgtg
     5041 agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag caaaaagatt
     5101 aatgcgtacc gtaacggtgt agccattctt gttccagcgc atttacccaa cctgcgtgcg
     5161 gttcagccag taccggcggc agatcttgcg gcattcttga ttgcaatgtt tcgcgctgcc
     5221 catcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacagcg ggatctgctt
     5281 tacgcaattg cgcatcgggt gagagcaaga aattggcaac taccttcgcc gcagcactgg
     5341 cattcgcgtt ggcaggaatg gtgacgaaat gcacgttgcc aatcatcccc tcgcgaaagc
     5401 caaaactgta actgcttgca ggcaaatcgc cgctggcgat tttttgctgc gcatgcgcgg
     5461 ggttaaaggt cagcgacagg cgaaatgttc cggctttcag cagagcatcc atccgcgcgg
     5521 gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagcgca tcaagatatt
     5581 gccacaaggg agcagtgaca cgggcgaaag tcgcatcgtt cggcgcttca tttaatgcgg
     5641 cgggatcggg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg gtaaagtccg
     5701 gtgggcgcgg ataggtaacc gtgccgggat tggctttagc aaactccagt aaggcttgcg
     5761 gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaatgtcagt tgtgcgcccc
     5821 cccagggcga ttcagcccct tctgtaggca ctgaaaaatc ttcccgcacc ggcagctgtg
     5881 tgtcgacata gcgccagttg ggcagagtct ctgcccagtc ggtttgcagt aaattggcct
     5941 cttttaaggt gcggaagttt tcgccgttca cccagagcag atccaccgag ccgcccgttt
     6001 tacgtccggc agcagcttcg gtctgaatgc gcttcaccgc gtctgcggca tccgccagac
     6061 gaacaatctt caggtttata gcgtaatggg ttttcatctc gccgctaacc cagtcgagat
     6121 agcggttaat tgcggtatcg ccgccccagg cgttaaacca gacagtttgc cctttggcct
     6181 catttttaat ttcttgccag tcactggcat gtgttgccag agaaaacagc gataacaatc
     6241 ccagcaacca cccacaatgg cgcatcatct ctccttaaga acgtttttcc ggaatacggc
     6301 acagcccctg agcgggaatg tccatggcag aattaaaacg ggctgacaga ttttgcaggc
     6361 cgatcaatgc cgtcagttcg gtgagctcct gagcgtcaaa atgcgcagcc agtcgggtac
     6421 gcagggcatc atcgaccgtt ggcggcgtta cgcttgcggc ttcggcgtac tccagcgcca
     6481 gccgttcttc atcgctaaag agcgggcttt gccgccagtc agccactgcc agcagtttat
     6541 cgctgctgcc ggtgcgctcg gcgactttca tactggtgat atccacacaa aactcacaca
     6601 ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgtac taccggatcg agcggtgagc
     6661 gtttgcgctc cagccagcca acaaacatcg acaccagata aaagataaac gggatccgcc
     6721 cccaccagcg aatggggctt agcacctcgc cgtaatggcg acgatgcagc cacgcctgcg
     6781 gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcaggcat tcctcttctg
     6841 gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa acagaataaa
     6901 cagtccggtg acaaaccaga aggtaccgcc tgttaacatg ctgcccgccc aggaataaac
     6961 aatagtcgcc ggtaactgac caagcccggt ggcgataaaa aacgagcgaa aacgtattga
     7021 agtcaaaccg gcagcatagc tgattggatc gaaagggaca aaaggcaata accgacagac
     7081 caggatggtg tgtttgccgt agcgagtgaa aaagccgtcc atactgtcaa gtacggtttt
     7141 gccggttaat ttttccacca cttcgcggcc catcactctg gcgataaaaa agcacagtgc
     7201 cgcgccggcc atcgaactgg tccacgacag cgagccgccc cagaacgcgc caaacagcga
     7261 cgcattggca aaggtgatca aaaacgcagg tagcggtgca gcaatggcct gtaaaatcat
     7321 caacaagaac gagacaactg ccgccagtgc gccgtaagac tgaataaagc gttctatacc
     7381 ctgttggtcc acggcggcaa acgccgcaac gctgcgattg ataaactcat ggacgccggg
     7441 aagcagcgcc catgccagca gcatagcgaa caacaggatg atgagggtta tacggtagta
     7501 ccagatttta cgcgattgca tcttcatcat cctttatcct ggctttattc ggtgatttta
     7561 aaggtaaccg ttgccagcgt gttattcgcc gggagaaccg aggcattgcc tttgaattta
     7621 acttcacctc gtttttcaac cgcaccataa gtgtatgttg cattgctgac gatgccgacc
     7681 gggcagctat ccagacacac caggcaaccc gttttcttct cttctgctgc cgttaaatta
     7741 ccgccaaagc gcatgtccag tttcttgcca ttactgtcaa caatcacttc atcgaaggaa
     7801 tacgtttttg ccgccccttg ccagttgacc gaaatatcca gtttgctgcc tgtgacatga
     7861 gtcgtttctt tattatccat cgtcatgttt tcgcccggtg taccacctgc ctctttcagg
     7921 gcttcataaa atgctttcgg ggtcgcataa cccataaaca gcgatttatg tccgttggag
     7981 ccatctttaa acacaatacc gtgacgagtg tcgtcggtga ggaaacgacc attaatttgc
     8041 accagcatgg ttaccgtatg gttattggca tcgacgcgca gcggttggct gttcgaaagt
     8101 ccgtcgtatt ccacttttgc cgccgcattc tctttttgat cgcagccgct aagcccagca
     8161 acagccagta agcagagtgc agtcagtcct tttttccatg acactgaata atgttgcaac
     8221 atcgttctat ccttcatttt ttgggtgagt aagtgggttg cggcgcgaag cagacagatc
     8281 cacatgtttg tgtcgggcgt agagttttgc gagctggacg agaataaaca gcgccaggcc
     8341 cgccagacag agttgtaaaa taaagcgcag cgtaatgcct tcattggcga gatcgcttgc
     8401 catcacggta taaataacaa taccgggcag ggtcgtgagt gccgaaataa gggtataagg
     8461 ccagaaggcg attgtggtta atccgtaagc gtaattttga atattgtaag gaaacaacgg
     8521 gattaagcgg gtcagaataa gaaaatcaat accgttacgc gcaatgcctt tttcaatggc
     8581 ctggaaggta tggctatggc caacgtattt cagcagtaaa tcacgtccca tccagcgcgc
     8641 cagcaggaat gagcacgagg aggccagcgt ggcggcaatt aatgagagta gtgtccctaa
     8701 gaacgggcca aaaactattc ctcccgcgat caccaatatg cttcctggta acagaaagag
     8761 ggtggcaatg atgaataaca gaatatagag gctataaccg aacaatccgc tctggcgaat
     8821 gagtgtctgt aagtggggta aatcggtgag cagatcgaat aaaccgaaag cgtggatagc
     8881 gtaaatggcc agcgcaaaaa taagacaggc aaaaagaaat ttacgctcag cgttcatcgt
     8941 ggagtttcct atatcaaact tgataataga gaagtggtca gacaactaaa aacgcagtga
     9001 cggtataaca aaggacggca ggcaacaaat cattactgac agtttgttga tttttctcaa
     9061 ggttaattct cctgacccag tttgagccag gagagctgct aaattagcgg cggaaggtcg
     9121 cccagacggg ggcgtgatcg gacggttttt ccatgctgcg gatttcatag tcgatgccgg
     9181 tttctacgca acattctgcc agcggttggc tggcgagcag caggtcgatg cgcagaccac
     9241 ggttatcgtc aaaacctttt gagcggtaat caaaccatga gaaacgatct gctgtttgcg
     9301 gattcgcatg gcggaaggta tcaaccaatc cccagctcat cagtctctcc atccattcgc
     9361 gttcctccgg aaggaaagaa cattttccgg tacgcagcca gcgcttacgg ttctcttcgc
     9421 caatgccaat atccaggtcg ccaggactga tattcatatc gcccataatc agcaccggat
     9481 tttcacgctt gagttcggtt tccaggtagt tttgcagatt ctgataaaac tgcgcctttg
     9541 ccgggaattt tatcggatgg tcgcggcttt caccctgcgg gaagtaaccg ttgatcacgg
     9601 tgacattgcc cagcggcgag gggatttccg ccataataat ccgccgctgc gcctcttctt
     9661 cgtcaccggg aaagccgcga cgcacggcaa tcggcgtctc tttggtcagc agcgccacgc
     9721 cataatggcc tttctgcccg tgataaaaca cgttgtagcc gagcttcgcc acctcttcga
     9781 ggggaaacat atcgtcatga acttttgtct cctgcaggcc aatcacatcc ggttggtgct
     9841 tttcgacgat ggcttcaagc tggtggggtc tggcgcgcag gccattgata ttaaaagaga
     9901 caaatttcat agtcgctgcc atttattcag ataaagagtg cgtggatggt agcagaattt
     9961 cgctttgctt accgcctgtt gttaagtgat ttgatcgatg gttgttaatg atgtcaacga
    10021 tggcgcaaaa aatgcccgct tttggtgcgc gctgcgtcag aatggcgcag taat
//
LOCUS       DBFIFS010000159        10031 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.159, whole genome shotgun sequence.
ACCESSION   DBFIFS010000159 DBFIFS010000000
VERSION     DBFIFS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.159"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            124..1182
                     /gene="citC"
                     /locus_tag="N7A07_003490"
     CDS             124..1182
                     /gene="citC"
                     /locus_tag="N7A07_003490"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU2478087.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1197..1493
                     /gene="citD"
                     /locus_tag="N7A07_003491"
     CDS             1197..1493
                     /gene="citD"
                     /locus_tag="N7A07_003491"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU2478088.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1490..2398
                     /gene="citE"
                     /locus_tag="N7A07_003492"
     CDS             1490..2398
                     /gene="citE"
                     /locus_tag="N7A07_003492"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU2478089.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2409..3941
                     /gene="citF"
                     /locus_tag="N7A07_003493"
     CDS             2409..3941
                     /gene="citF"
                     /locus_tag="N7A07_003493"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU2478090.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3945..4496
                     /gene="citX"
                     /locus_tag="N7A07_003494"
     CDS             3945..4496
                     /gene="citX"
                     /locus_tag="N7A07_003494"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU2478091.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4471..5349
                     /gene="citG"
                     /locus_tag="N7A07_003495"
     CDS             4471..5349
                     /gene="citG"
                     /locus_tag="N7A07_003495"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU2478092.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5400..6863
                     /gene="citT"
                     /locus_tag="N7A07_003496"
     CDS             5400..6863
                     /gene="citT"
                     /locus_tag="N7A07_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU2478093.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6977..7783
                     /gene="rna"
                     /locus_tag="N7A07_003497"
     CDS             6977..7783
                     /gene="rna"
                     /locus_tag="N7A07_003497"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU2478094.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8013..8423
                     /gene="rnk"
                     /locus_tag="N7A07_003498"
     CDS             8013..8423
                     /gene="rnk"
                     /locus_tag="N7A07_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU2478095.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8561..8650
                     /gene="yldA"
                     /locus_tag="N7A07_003499"
     CDS             8561..8650
                     /gene="yldA"
                     /locus_tag="N7A07_003499"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU2478096.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8654..9892)
                     /gene="ybdR"
                     /locus_tag="N7A07_003500"
     CDS             complement(8654..9892)
                     /gene="ybdR"
                     /locus_tag="N7A07_003500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU2478097.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2335 a         2705 c         2559 g         2432 t
ORIGIN      
        1 aaaaagatat acctcgatca cccccctttc tcccaagtga aaataaaagg ttatcagttt
       61 gcaacattga acaacattcg ttgcaaatcg ataacaacat gcaccttcag gatactattt
      121 attatgttcg gcaatgatat tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg
      181 gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg acaccacagt cgaagtattt
      241 attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg gaattgccgg aaatattatt
      301 aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac tggcgctgac attagccact
      361 gaactgataa acctcgccta tgagcggcac agcacgcatc tgtttattta taccaaaacc
      421 gaatacgagg cgctgttccg ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg
      481 atggtgctga tggaaaacag cgccacgcga ctgaaacgct atgccgaatc gctgaaaaaa
      541 tttcgtcatc cagggaacaa gattggctgc attgcgatga acgccaatcc ctttacgaat
      601 ggtcaccgtt atctgattca acaagctgcg gcacagtgcg actggttgca tctgttttta
      661 gtcaaagaag attcttcacg cttcccctat gaagaccggc tggatctggt gttaaaaggc
      721 accgccgata ttccacgcct gactgtgcat cgcggctccg aatacatcat ctcccgcgct
      781 acgttccctt gctacttcat taaagaacag agcgtcatta accattgtta caccgaaatt
      841 gatctgaaga ttttccgtca gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc
      901 ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc aggatatgcg ctactggctg
      961 gaaacgccga ctatctccgc agcgcccatc gaactggttg aaattgagcg gctgcgttac
     1021 caggagatgc cgatatccgc ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg
     1081 gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt tgcagaacct gcttgagcac
     1141 tcccgccagg acgcggcagc tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga
     1201 aaataaacca gcccgccgtt gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg
     1261 ccccactcga tacgcaggat atcgacctgc aaatcaatag cagcgttgag aaacagtttg
     1321 gcgatgcaat tcgcaccacc attctggacg ttctcgcccg ctacaacgtg cgcggcgtac
     1381 agctgaatgt cgatgataaa ggcgcactgg actgcatttt acgtgcacga ctggaagccc
     1441 tgctggcacg cgccagcggt atcccggctc tgccatggga ggattgccaa tgatttccgc
     1501 ttcgctgcaa caacgtaaaa ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa
     1561 tgccgcgatg gtcagcaact ccttcatcta cccggctgat gccctgatgt ttgacctcga
     1621 agactccgtg gcattacgtg aaaaagacac cgcccgccgc atggtttatc acgcgctgca
     1681 acatccgctg tatcgcgata ttgaaaccat tgtgcgtgta aacgcgctgg attccgaatg
     1741 gggtgttaac gacctggaag ccgtcgttcg cggtggtgcg gacgttgtac gtctgccgaa
     1801 aaccgatacc gctcaggatg ttctggatat cgaaaaagag atcctgcgta tagaaaaagc
     1861 ctgtggtcgt gaacccggca gcaccggcct gctggcggcg attgaatctc cgctgggcat
     1921 tacccgcgca gtggaaatcg ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc
     1981 agaagactat gtgcgcaacc tgcgtacaga acgctccccg gaaggaactg aactgctgtt
     2041 cgcacgctgt tccattttgc aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt
     2101 ctattccgac gctaacaacg aagccggatt cctgcaagaa gccgcccaca tcaaacagct
     2161 gggctttgac ggcaaatcgc tgatcaaccc gcgtcagatt gatctgctgc acaacctcta
     2221 cgcaccgacc cagaaagaag tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc
     2281 cgctcgcgaa ggcctcggcg tggtttccct gaacggcaag atggtggacg gtccggttat
     2341 cgatcgtgcc cgtctggtgc tctcccgtgc agaactttcc ggcatccgcg aagaataagg
     2401 caatcaaaat gacgcagaaa attgaacaat ctcaacgaca agaacgggta gcggcctgga
     2461 atcgtcgcgc tgaatgcgat cttgccgctt tccagaactc accaaagcaa acctaccagg
     2521 ctgaaaaagc gcgcgatcgc aaactgtgcg ccaacctgga agaagcgatt cgtcgctctg
     2581 gtttacagga cggcatgacg gtttccttcc atcacgcttt ccgtggcggt gacctgaccg
     2641 tcaatatggt gatggacgtc atcgcgaaga tgggctttaa aaacctgatc ctggcgtcca
     2701 gctccctgag tgattgccat gcgccgctgg tagaacacat tcgccagggc gtggttaccc
     2761 gcatttatac ctccggcctg cgtggtccac tggcggaaga gatctcccgt ggtctgctgg
     2821 cggaaccggt gcaaatccac tctcacggcg gacgtgtgca tctggtacag agcggcgaac
     2881 tgaatatcga cgtggctttc ctcggcgtcc cgtcctgtga tgaattcggt aatgccaacg
     2941 gctacaccgg taaagcctgc tgcggctccc tcggctatgc aatggttgat gccgacaacg
     3001 caaaacaggt tgtgatgctt accgaagaac tgctgcctta tccgcataat ccggcaagca
     3061 ttgagcaaga tcaggttgat ttaatcgtca aagttgatcg cgttggcgat gctgcaaaaa
     3121 tcggcgctgg tgctacccgt atgaccacta acccgcgcga actgcttatt gcccgtagcg
     3181 ctgcggatgt gattgtcaac tcaggctact tcaaagaagg tttctccatg caaaccggca
     3241 ccggcggcgc atcgctggcg gtaacccttt tcctggaaga caaaatgcgt agccgcgata
     3301 ttcgcgccga cttcgccctc ggtggtatta ccgcgacgat ggttgatctg cacgaaaaag
     3361 gtctgatccg caaactgctg gatgtacaga gctttgacag ccacgctgcg caatcgctgg
     3421 cccgtaaccc caatcacatc gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag
     3481 gtgcatcggt tgatcgtctc gacgtggtgg tactgagcgc gctggaaatt gacacccagt
     3541 tcaacgttaa cgtgctgacc ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact
     3601 gcgataccgc gattgcctct gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta
     3661 ttccgactct ggtggataac gtgctgacct gcatcacccc gggctccagt gtcgatattc
     3721 tggtcacaga ccacggtatc gcagttaacc cggcacgtcc ggaactggca gaacgtctgc
     3781 aggaagcggg cattaaagtg gtttccattg agtggctgcg cgaacgtgcg cgtctgctga
     3841 ccggtgaacc acagccgatt gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg
     3901 atggctcggt gatcgatgtt gtgcatcagg tgaaggaata agccatgcac ctgcttcctg
     3961 aactcgccag ccaccatgcg gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc
     4021 aagcacggca acacgtctgg ctcaagcgcc atcctgttcc actggtctcc tttaccgtgg
     4081 ttgcgcctgg gccgattaaa gacagtgagg tcacacgccg aatttttaat catggcgtga
     4141 cagccttgcg tgccttagcc gcaaaacagg gctggcaaat tcaggagcag gctgcactgg
     4201 tttccgccag cgggccggag ggtatgttga gcattgccgc cccggctcgc gacctcaagc
     4261 tcgccaccat tgagcttgaa catagtcatc ctctcgggcg gttatgggat atcgatgtcc
     4321 tgacgcccga aggcgaaatt ctctcccgcc gcgactattc actgccgcct cgccgctgcc
     4381 tgttgtgcga gcaaagcgca gccgtctgcg cgcgtggaaa aacccatcaa ctgaccgatt
     4441 tactcaaccg catggaggca ctgctgaacg atgtcgatgc ctgcaacgtt aactaaaacc
     4501 acaaagcttg cgacgtcatt aatcgatgag tacgccctgc tgggctggcg cgccatgctg
     4561 actgaagtca atctgtcacc gaaaccaggt ctcgtggatc gcattaactg cggtgcgcat
     4621 aaagatatgg cgctggaaga tttccaccgc agcgcgctgg cgattcaggg ctggctaccc
     4681 cgtttcattg aatttggtgc ctgtagtgcg gaaatggcac cagaagcggt actccacgga
     4741 ttacgcccaa ttggtatggc ttgcgaaggt gatatgttcc gcgccaccgc gggcgtaaac
     4801 acgcataagg gcagcatttt ttctttaggg ctactgtgtg cggcaattgg ccgtttgctt
     4861 cagctcaacc agttgctaac gccaacaacc gtttgttcta cggcggcaag tttctgccgt
     4921 ggcctgaccg atcgcgaact gcgtaccaat aattcacaac tgacggcagg tcaacggttg
     4981 taccagcagc ttggccttac cggcgcacgc ggtgaagccg aagcgggtta tccactggtg
     5041 atcaaccacg ccttgccgca ttacctcact ctgctggatc aggggttaga tcctgaactg
     5101 gcattgctcg ataccttgct cctactgatg gcgatcaacg gcgataccaa cgttgcttcg
     5161 cgcggtggcg aggggggcct gcgctggcta cagcgcgagg cgcaaacatt attgcaaaaa
     5221 gggggcattc gaacccccgc agatctcgat tatctccggc agttcgacag ggagtgtatc
     5281 gaacgaaatc tcagtccagg cggcagtgcc gacctgctga tccttacctg gtttttagca
     5341 cagatttaat tatttaagaa cttgataaat ttggaaatat taattttcgg agaacccgta
     5401 tgtctttagc aaaagataat atatggaaac tattggcccc actggtggtg atgggtgtca
     5461 tgtttcttat ccctgtcccc gacggtatgc cgccgcaggc atggcattac ttcgctgtgt
     5521 ttgtggcaat gattgtcggc atgatcctcg agccaattcc ggcaacagcg atcagtttta
     5581 ttgcggttac tatttgcgtt attggcagta attacctgct ctttgatgcc aaagaattag
     5641 ctgacccagc gtttaatgcg caaaaacagg cgctgaaatg gggcctggct ggtttttcca
     5701 gcactacggt atggctggta tttggcgcat ttatttttgc attagggtat gaagtttccg
     5761 ggttaggtcg tcgcattgcc cttttcctgg tgaaattcat gggcaaacgc acgctgacgt
     5821 tgggttatgc gattgtcatt atcgacattc tgctggcacc gtttacaccg tccaacaccg
     5881 cgcgtaccgg gggtacggtt tttccggtca ttaaaaacct gccgccgttg tttaaatcat
     5941 tcccgaacga tccgtccgcg cgtcgtattg gcggctattt gatgtggatg atggtcatta
     6001 gtaccagtct gagttcgtcc atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg
     6061 agttcgtcag caaaattgcc ggtatccaga ttagctggtt gcagtggttc ctctgcttcc
     6121 tgccggttgg ggttatcttg cttatcattg cgccgtggct ttcctacgtg ctgtacaaac
     6181 cggaaatcac acacagtgaa gaagtggcaa cctgggcggg tgatgaacta aaaaccatgg
     6241 gtgcgctgac acgcagagag tggacgctga ttggccttgt attgctcagc ttaggtttgt
     6301 gggtatttgg cagtgaagtc attaatgcta ctgcggttgg tctgctggca gtttcgctaa
     6361 tgctggctct gcacgttgtg ccgtggaaag acattacccg ctataacagc gcatggaaca
     6421 cgctggtcaa cctggcaact ctggttgtga tggctaacgg cctgactcgt tctggtttta
     6481 ttgactggtt cgccggtacc atgagtacgc acctggaagg attctcacca aacgcaacgg
     6541 tgattgtact ggttctggtg ttctactttg cacactacct gtttgccagc ctgtctgcgc
     6601 acaccgcaac catgctgccg gttattctgg ccgtcggtaa aggtattccg ggcgtaccaa
     6661 tggaacaact gtgtatcctg ctggtgctgt ctatcggtat catgggctgt ctgacgccgt
     6721 atgcaaccgg tcctggggtg attatttacg gctgtggcta tgtgaaatca aaagattact
     6781 ggcgtcttgg cgcaatcttc ggggtgattt acatctctat gttgctgttg gttggctggc
     6841 cgattctcgc catgtggaac taatacgttc gccaaacgct gaataaatca tcacgcccgc
     6901 caggttagcc cacctggcgg gcttttttat gatttaatag atagtattca gtgtatgtat
     6961 gagttccaca cccattatga aagcattctg gcgtaacgcc gcgttgctcg cggtttctct
     7021 gcttcccttc tcttctgcca acgccgtagc gttgcaggca aaacagtatg gcgattttga
     7081 tcgctatgtc ctggccctct cctggcaaac cggattttgc cagagtcaac acgatcgaaa
     7141 tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg accaacaaag ctgattttct
     7201 gaccgtacat ggtctgtggc caggattgcc taaatcggtt gctgcccgtg gtgttgatga
     7261 acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc ccgaatctac cagaagcgcg
     7321 cgccagccgg atgtgttcat cgccggaaac cggattatca ctggaaacgg ccgctaaact
     7381 aagtgaagtc atgccaggag ctggcggacg ttcctgcctg gaacgctacg aatatgccaa
     7441 acacggtgcc tgctttggtt ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa
     7501 tcaagagata aaagagagcg aagccggaaa attccttgcg gataattacg gtaaaacagt
     7561 gagccgccgt gactttgacg ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc
     7621 agttaagcta acgtgccagg gtaaccctgc gtatttgact gaaattcagg tctcgatcaa
     7681 agctgacgcc atcaacgctc cgctttctgc aaactcattc ttgccacaac cacacccagg
     7741 taactgtggc aaaacctttg tgattgataa agcgggttat taatcctctg atttacaaaa
     7801 cacgctaatt cgtctttcat aaaccatgac gattaagaat tatacataat gcgctgcatt
     7861 gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc
     7921 atgtgagtta aaccctcgcc gcctgacggt gagggttttc ttttgggtat gtttatctgc
     7981 gacactcgca gtaccgacga catggagtaa aaatgtccag accaactatc atcattaacg
     8041 acctggatgc cgaacgcatc gatattctgc tggagcaacc cgcctatgct ggtttgccaa
     8101 tcgccgacgc gttaaacgca gagttggatc gcgcccaaat gtgttcgcca gaagagatgc
     8161 cacacgacgt ggtgacaatg aacagccggg ttaaattccg caatcttagc gatggcgaag
     8221 tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga tagcaatact cagctttccg
     8281 ttatggctcc ggtaggtgcc gcactgctgg gactgcgcgt tggcgattcc attcactggg
     8341 aacttccggg cggcgttgca acccaccttg aagtgctgga actcgaatac cagccagaag
     8401 ctgctggcga ctacctgctt taatcccatc tgtacaagcg ataactcgct ttgcccagag
     8461 gatgcactcg catcctcttt atgcaaaatc agcaattttt cctggttctc actgttcact
     8521 tttgctgcaa aattattccg tttcgtttgt gggaataacc atggcagaag cattttacat
     8581 cctgatagga tttctgatta tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa
     8641 ccactcctga cgctcatatt gttccccccg gcatcgcatt caccagacct gaaaccgcct
     8701 tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat caccttacgg cactcctctt
     8761 cacgcttttc aaagatctca tatccccggg cggcctcttc aaacggcata tagtgggtaa
     8821 caatttcttc tggtttcagt aatcctttct caattaacgg taataattct cccagccatg
     8881 cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt atcaaaggcg tcgccaaaca
     8941 ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct aacaatgccg ccacgcctga
     9001 ccgccgcaat acactgacgc aacgctttac cgctgctgcc ctccagtttc aggttagtca
     9061 gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac ggcgtctatt actgcatcca
     9121 cgccccggtg acctgccgtt tgttcaataa ttgactgtgc cggatcgcta tcttcatcga
     9181 aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg caagcggtag ggatgatgat
     9241 caacaacaaa aatctgttcc gcaccgagca accgtgcaca ggcgattgtc aacaatccca
     9301 caggaccggc accatagaca gcaacgcttg aaccttgttg gatctgcgca ttttttgctg
     9361 cctgccatgc cgttggcaga atatcagaaa ggaaaagggc tttatcatct gaaagcaaag
     9421 gcggtacttt aaacggcccc acattccctt tagggacacg gacatactcc gcctgtccac
     9481 caggaacgcc accatacagg tgactataac caaacaatgc cgcgggagct ggtatctgtt
     9541 ttttattgag cgcagcgcct ttacccgcat tggtattttc gcaggcagca tattgctgca
     9601 gtcgacagaa aaaacagtcg ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt
     9661 tttgcaaatt ttttacatcc tttccggttt caactacttc ccccataaat tcatgaccaa
     9721 aaatatcgcc atgtttaacc tggggtattt tgcctcgata aagatggagg tcagagccac
     9781 agatcgccgt tgccgttata cgcagaataa tatcatctgc ctgttcaatg cccggatcgg
     9841 gaacattttc tacctgaacg tgatgtgggc cgtgataagt caatgctttc atgcaacctc
     9901 cagagacagt tcaaaaaaga gtcagtcttt aagggtagct atcacagcgg gaaaacgggc
     9961 ggtaccgcag gttttgggag aattcctggt gttcagtgag aatatattca tcacagaaac
    10021 agttcgtaag g
//
LOCUS       DBFIFS010000160        10016 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.160, whole genome shotgun sequence.
ACCESSION   DBFIFS010000160 DBFIFS010000000
VERSION     DBFIFS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.160"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            30..1079
                     /gene="allD"
                     /locus_tag="N7A07_003501"
     CDS             30..1079
                     /gene="allD"
                     /locus_tag="N7A07_003501"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU2478098.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1101..2336
                     /gene="allC"
                     /locus_tag="N7A07_003502"
     CDS             1101..2336
                     /gene="allC"
                     /locus_tag="N7A07_003502"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU2478099.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2347..3132
                     /gene="allE"
                     /locus_tag="N7A07_003503"
     CDS             2347..3132
                     /gene="allE"
                     /locus_tag="N7A07_003503"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU2478100.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3360..4505)
                     /gene="glxK"
                     /locus_tag="N7A07_003504"
     CDS             complement(3360..4505)
                     /gene="glxK"
                     /locus_tag="N7A07_003504"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU2478101.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4527..5828)
                     /locus_tag="N7A07_003505"
     CDS             complement(4527..5828)
                     /locus_tag="N7A07_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGU2478102.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTEVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5885..7246)
                     /gene="allB"
                     /locus_tag="N7A07_003506"
                     /pseudo
     CDS             complement(5885..7246)
                     /gene="allB"
                     /locus_tag="N7A07_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(7283..8674)
                     /locus_tag="N7A07_003507"
     CDS             complement(7283..8674)
                     /locus_tag="N7A07_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU2478103.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(8843..9721)
                     /gene="glxR"
                     /locus_tag="N7A07_003508"
     CDS             complement(8843..9721)
                     /gene="glxR"
                     /locus_tag="N7A07_003508"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU2478104.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9822..>10016)
                     /locus_tag="N7A07_003509"
     CDS             complement(9822..>10016)
                     /locus_tag="N7A07_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU2478105.1"
                     /translation="QWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVG
                     CEYKPQTTTEAGLRWMDPYR"
BASE COUNT         2664 a         2648 c         2389 g         2315 t
ORIGIN      
        1 cttcacaccg tcgttaaaaa ggaagacaga tgaaaatcag tcgggaaaca ctccaccagc
       61 taattgagaa taaactctgc caggctgggt taaaacgtga gcacgctgca accgtggctg
      121 aagtattggt ttatgccgat gccagaggga tccactctca tggcgcggtg cgcgtggaat
      181 actacgcgga acgcatttca aaaggcggca ccaaccgcga accggagttt cgtcttgagg
      241 aaaccgggcc gtgctcggca attttacatg ccgacaatgc cgccggacag gtcgcggcga
      301 aaatgggtat ggaacatgcc atcaaaaccg cccagcaaaa tggcgttgcg gtggtcggta
      361 tcagccggat gggtcacagc ggcgcaatct cttattttgt gcagcaggca gcccgcgccg
      421 gattaattgg catttcgatg tgccagtccg atccgatggt ggtgccgttt ggcggcgcgg
      481 aaatttacta cggtactaac ccactggcct ttgccgcgcc gggagaaggc gacgagatcc
      541 ttacctttga tatggcgact accgtacagg catggggaaa agtcctcgac gcccgctcgc
      601 gtaatatgtc tatcccggat acctgggcgg tcgataaaaa cggtgcacca acaaccgatc
      661 cgttcgcggt acatgctctg ctccccgcag ccgggccgaa agggtatggc ctgatgatga
      721 tgattgacgt cctctcaggc gtcttactcg gcttaccgtt cgggcgacag gttagttcga
      781 tgtatgacga tttacacgcc gggcgtaatt tggggcaatt acatgtagtt attaacccga
      841 actttttctc ctccagcgaa ttattccgtc aacatcttag ccagaccatg cgcgaattaa
      901 atgccattac ccccgcgccc ggttttaatc aggtttatta tcccggacag gatcaggata
      961 ttaaacaacg ccaagccgcc gtcgaaggca tcgaaattgt tgatgatatt taccagtatt
     1021 taatttccga cgcgctttat aacacgtcat acgaaacgaa aaatcccttt gcgcaataat
     1081 tattgagaca ggactttctt atgattacac attttcgtca aaccatagaa gaagcgctgc
     1141 cctggctttc ctcttttggc gctgacccag cgggtgggat gacccgttta ctttattcgc
     1201 cggaatggct ggaaacccag cagcaattaa aaaaaagaat ggccgcaagt gggctggaaa
     1261 cacgtttcga tgaagtgggg aatttatacg gtcgcctgag tggcaccgaa tatccacagg
     1321 aagtgattct gagcggttcg catatcgata ccgtggttaa cggcggtaac ctcgacgggc
     1381 aattcggcgc gctggcggcg cggctggcaa ttgactggct gaaaactcaa tacggcgcac
     1441 cgctgcgtac ggttgaagtg gtggcgatgg cggaagaaga aggtagccgc ttcccgtatg
     1501 ttttctgggg cagtaaaaat atctttgggc tggcgaatcc tgacgacgtg cggaatatct
     1561 gtgatgccaa aggaaatagt tttgtcgatg cgatgaaggc ttgcggattt actctgccgg
     1621 acgccccgct aactccgcgt caggatatta aagcctttgt cgaactgcat atcgaacagg
     1681 gctgtgtgct ggaaagtaat gggcaatcaa ttggcgtggt gaatgcaatt gtcgggcaac
     1741 gtcgctatac ggtgacgctg aacggcgaat caaaccatgc aggcaccacg ccgatgggtt
     1801 atcgtcgtga tacggtttac gctttcagtc gcatttgcca tcagtcgatc gaaaaagcga
     1861 aaaagatggg cgatccgctg gttctgacct ttgggaaagt agagccgcgc ccgaatacgg
     1921 tgaatgtggt gccgggtaaa accacgttca ccattgattg tcgtcatacc gacgccgccg
     1981 tgctgcgtga tttcacccaa cagttagaaa acgacatgcg ggcgatttgc gatgaaatgg
     2041 acattcgtat tgatatcgat ttatggatgg acgaagaacc cgtgccgatg aataaggacc
     2101 tggtcgccac cctgacagaa ttgtgtgaaa gtgaaaaact gaattaccgg gtgatgcaca
     2161 gtggtgccgg gcacgacgcg caaattttcg cgcctcgcgt accaacctgc atgattttta
     2221 tccccagcat caacgggatc agccataacc cggcggaacg caccaatatt accgaccttg
     2281 ccgaaggggt caaaacgttg gcactcatgc tttatcaact tgcctggcag aaataaggag
     2341 tcataaatgg gatatttaaa taacgtcacc ggttaccgcg atgatttact ggctaaccgt
     2401 gcgattgtga aacacggtaa tttcgcactg ttaacaccag acggtctggt aaaaaatatt
     2461 attccgggct ttgaaaattg tgacgcgaca atcctctcca cgccaaagct gggtgcctct
     2521 tttgttgatt atctggtcac actgcatcaa aacggcggca accaacaggg cttcggtggc
     2581 gaaggcattg aaacgttcct gtatgtgatc tctggaaata tcactgccaa agccgaaggc
     2641 aaaacatttt ccttaagcga aggtggctat ctttattgcc cgcctggctc cttaatgacg
     2701 tttgttaacg cccaggccga agacagccaa atctttttat ataagcgccg ctatgttccg
     2761 gtagaaggct atgcaccgtg gctggtttct ggcaatgcca gcgaactgga acgcattcac
     2821 tatgaaggta tggatgatgt gatcctgctg gatttcctgc ccaaagagtt aggttttgat
     2881 atgaacatgc atatcctttc ttttgcacca ggtgccagcc acggttatat cgaaacacat
     2941 gttcaggaac acggtgccta tattctttcc ggtcaggggg tttataacct cgacaataac
     3001 tggatcccgg tgaaaaaagg cgattacatc tttatgggcg cttattcttt acaggctggt
     3061 tatggcgtag ggcgtggtga agcgttcagc tatatttact cgaaagattg taaccgcgac
     3121 gtagagattt aagtcatacc actcgcctga ttatttttag ctatgttgga ttttgccacg
     3181 gtttataccg tggctttttt tgcaacatag cctgatgcgc ttcgcttatc aggcctacat
     3241 gatctctgca atatatagaa tttgcgcgat tttcaggaag gatatggtac tcatgccgca
     3301 tccggcatga gtactgcact cttatgcgat ataaatcgca tccgctttaa aggtaagggt
     3361 cagtttttaa ttccctgacc tattttaatg gcgcaggcaa tatttcgcgc gctgttgaag
     3421 agattggttt caccgctggc gagcacttcg gctaaaggtg ccagacgtgg caaaatgctg
     3481 aataccgcat caatgccgta ctggtgcacc acttccacgc catcacccaa tacgccagca
     3541 atcccaatca ccggtacatt aaactgcttc gccaccgacg caacgcccag cggcgcttta
     3601 ccgcctgccg tttgcgagtc aatgcgccct tccccggtga tcaccagtgc tgccccctgc
     3661 accgcctgct ccaggttgac cgcccgcagt acaatctcaa tacccggttt aatatccgca
     3721 ttgaggaata ccgccgccgc aatccccata ccgcccgcag cgccaccgcc cgccatctgg
     3781 cagacattaa tttcagtttg ctgttgtaaa acacgggcgt aattttgcag cccctgttca
     3841 agttcttcga ccatctccgg cgttgcccct ttttgcgggc caaacaccgc cgccgcgccg
     3901 cgtgccccta ccagcgggtt atcaacgtca catgctactt caatatggca gttcgccaga
     3961 cgcggatcgc attcctccat ctcaatgctt gccacgcgcg ctaaattgcc gccgttagcc
     4021 accagcacct gaccgtcggc atcaaggaaa cgcacaccga gcgcctgcgc catgcccata
     4081 ccgccgtcga ccgtcgcact gccgccaatg ccgagaataa tatggcgaat gccgttatcc
     4141 agcgcatgac gaattaactc ccccgtacca aaactggagg ccagcaacgg attacgcttt
     4201 tcaggcgcga ccagcatcag gccacttgct gccgccatct caatcaccgc cgttttcccg
     4261 tcgccggtaa tgccataaaa agcattcact ttttcgccca tcggcccgca gacttcaagc
     4321 gtcacgatgt tgccgcccgt cgcggcgacc atcgcatcca ccgtcccttc gccgccatcc
     4381 gctatcggca aacagatata gttcgcatcg ggaaagaggg tcgaaaaccc ggctttaatt
     4441 gcctgacaac atttttctgc actcaagctc tctttaaaag agtctggcgc aatgacaatc
     4501 ttcattatca atcctttttc aacaaattat tcgatgcgtt cccacggaat aaggttatcc
     4561 ataagaacag ccagtaaaat cccgaccaat aagccgttac tgagcagagg acgaagcgtt
     4621 aatggaaggt cttgcagata cacaggcggt aatgccatta aaaatatgcc gacaaataac
     4681 ggcaatgcga gtcgataaat attgcgagcg gtaaacgtta tttgctggct aaacaccagc
     4741 gcggaaaaga gtaaaggtag ataagaaacc agcatgaccg cactactcac gggtaaaggg
     4801 atactgcaaa acagtcgcgt gagtgccgga accagcgcca ccagcaggca aataacgctg
     4861 ccataaataa acgaacgccg cgtgtaatcg ccagtctggg ttaataaacc aatggatgaa
     4921 acaaacggtg aaaatggaat tactgccagc ggcacggtta tcagtgtcat aaatccggtc
     4981 gccacaaagc tacgacgata acgcgtattc cccgcgccct gctgcggata aaaaacatcc
     5041 gtgccccgaa tcgcaccgta ggtattgctg atattcacca gacctgtaat cacctccgtc
     5101 agaataatcc ccggcgacaa agcaccgcca ctgccgagcg ggaaccactg ccagtgcaac
     5161 tcaccggaga gcgaatgcga agaaggaaag caaaagtacc acaacaacca gccggttatg
     5221 gtgccgacca gcaggccata acgggcaaaa cgttgcggca gaaagataat catcgccagt
     5281 accaggcaca tcaccgccac cgagagcgcg aacggcggta actgaatttt aaaattcggg
     5341 tcggctatgc caaacggcag cccgagcata cctttgaaaa agatagtggt cagctgcgcg
     5401 cccagcatca acataaacaa gaccatcacc gacggcgtaa ataaccgcgc caggcgatga
     5461 cctaatccgc taaaaccaat caacatcgtc agcacgccgg agagtgcaat ccccaccgcc
     5521 aggctggtag cgatatcgtt gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagtt
     5581 aggatggttc cccaccacag gccccccggc ccttccataa tagcgcgacg atgaccgcaa
     5641 aacgcctgag cgaagcaggc cagtgcggta gcaagaaaag catattgcgt gagcgtaagc
     5701 aggctacttt gcggcaactg aaaagcagaa agtagcgtag gaggaaccac aaccgtgttg
     5761 caaaaaataa agaaaaacca ctgaaatcct gataacaggc tttcgcggct aactgcaaaa
     5821 ttgaacataa acacacctta accggagaat gcccgccgca aggacgggca ttgcaggggc
     5881 cagattactg ctgatgttta aggataaatt gacctttcgg cgcaacaggg aagccctgtt
     5941 cgatgtcgta aatcacatca ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac
     6001 ggccaacata cgggctgact ttgtggcgat attccagatc gtcattggta agaacatagc
     6061 tgctattcgg ctgaatgaag acgaagtcgg catcttttcc tggggcgata cggccttttt
     6121 gctgcagacc aaaaatatct gctgcgttag tcgccattaa tttgccgaac attggcagag
     6181 acattccgcg tttctgtacc gcttcatcga acatcacgtc catacagttt tgcagaccgg
     6241 caataccgcc ccatgcttcc atgatgttgc cggctttcat ttccggaggg catggtgagt
     6301 ggtcggaaac caggcggtcg atttcaccgt taaacagttt ttcccacatg cctttctagt
     6361 tttccagatc gcggatcggc ggtgaacact tcgccagagt accgatttct tcgaactgat
     6421 cggtatccag tacgaagtaa tgcgggcagg attcacaagt aacgtcctga ccttcctgac
     6481 gtgcacgagt cacttcttca acgccttccg ggctgctgat atggcaaacg tgcagacggc
     6541 aaccggcaac tttcgccaga tacagcacgc ggcgaatcgc ttccacttcg gtaaataccg
     6601 gacgcgaagc cacatagtca tgggcagtta cgcgaccttc acgtttcgct tcttcgccca
     6661 gtgcgtcaca gatcagcgcg ttttcgcagt gcaccagcac cggctgcccc agttcgccca
     6721 gcttctgcgc acctttgaag aactgccagt cgtttacatc acggaagtcg ttgtcgatac
     6781 cgcgatcgcc acaggtcgca acgaagcatt tgaagccgac aacgcccact tcatccagct
     6841 catgcagacg gtcgatgttg taagacacca ggccaccgag ttgtgccgca tcgatagtca
     6901 gcttgccttt agcggcatcg aacttcagtt caattgaagc gcggtcaacc gttgcaggca
     6961 gctggttgag cggcatttcg atcatggtgg tgataccacc ttttgccgct gcgcgagtac
     7021 cggtttcata accttcccag tggctacgac ccggttcaga aatatgggtg tgcgcatcaa
     7081 ccatgcccgg cgaaaccacc agaccagacg catccataac ttcttttgca tcgcccagat
     7141 cctgaccgat agcagcaatt ttgccgcctt taacggcgat atctacaacg cgagcttcgt
     7201 tttctaaaat aacggtgccg tttttaatga ttaaatcaaa agacataaca aactccttgt
     7261 aaaatacaga ttggttttta actcaagcaa aggcgacgat gacgccgaca aaccatgaaa
     7321 cacgcgataa cggttccatt aagggaataa acttaccgcc aagagataaa ataacggcca
     7381 ccagagttac tgaaaacgcg gtgaggttaa aaccgttatc gtaatattta taatcgccag
     7441 gtgcggtgta cagttcatca agattaattt gcccacgcat caccacaaaa taatgcgcca
     7501 tcatgacacc aattaccgga ccaagcattc cgccgataat atcgaggaac agataaatac
     7561 tgtcctgatt ttccattaat ttccacgggc agatcagcag gctgataata ctggcaatca
     7621 gtacgccgtt tttataggtc agttttgtcg gtgcaatggc agcaatctga taaccagccg
     7681 gaataatatt accagtggcg ttagtggaga tagtcgtcat cagaataacc agtaccgcaa
     7741 agaacgaggc aaacaggctg tcccaacgct gaacaatatc cagcacgttc caggtatcag
     7801 cgccgtaatg aatactggct ccggcaataa tacatacccc cgcgaccgca aacagaatat
     7861 aggccacaac taaacccagc gtttgcccca gcgcttgctc acgaaacgag tgggcgtttt
     7921 gcgtaaagtc ggatgcgctc accgccggtg ccgcccagac cgcaactacc gcgttaatca
     7981 ccaccaggaa caagaatcca ctgttttctg ctttctgaat accgctcgga atgtagtcaa
     8041 agattggacc gagcccgacc agcgaaatcg cccaaatcgc cataccgccg aaaacgatat
     8101 agatgcacgg gttaagaatg gcagtgaatt tatttaaaac tttgccaccg ccaaaaccta
     8161 taccaacgtt gaccagccag aagagtaaga aagtaattaa gcccggtaga gaaaggccta
     8221 acagagtgaa atcaccaccg agagtcaaaa atcccggcca gattttgcca atcagaatca
     8281 agcaggccag tgaccccgcg taacattgca ggccaaacca cataatggca gcaataccgc
     8341 ctcttaataa tccgggaaac agtgcgccac gtacgccgta agaagcacgc aggatcatgg
     8401 caaacggtac gccgtattta ctgcccgcag cgccgtttaa taccattacc gcggcaatga
     8461 aaaaggcgct gaggataatt gccagcataa tactaaaggt agacaagccg agaataaaaa
     8521 agccgccgac catcacataa ttgggaacgt tatgaaccga acccatccat aaggtaaaat
     8581 agttaaatgt tttccaggtc cgctggcttt gcgttttcgg caatagatct tcgctatagc
     8641 cgcgttgctg gaatagtttt ctctgatgtt ccataaatcc ctcttaattt cacgtatcga
     8701 ataacgttgt tttaatctag ctatgaggaa aaaataaaat aaaaatatta tttttaatac
     8761 tgaagcaaca tctttggctg ctttcttttt taatattgct ggccgattca ttttttgata
     8821 tcggccattt ttattgcggg tatcaggcca gtttatggtt agccattaat tccagcgcct
     8881 gcactaacgc agagtgatcc aactggctgc caccgtttgc cgcgcaggta ttaaataact
     8941 cctggcaggt cgcagtgttt ggcaggttca gagccagtgc cttcgcactt tgcagtgcca
     9001 ggttgagatc tttctggtgc agcgcaattt tgaaacctgg attaaaggtg cgtttaatca
     9061 tgcgctcacc gtgaacttcc agaatacgtg aggaagcaaa gccgcccatc agcgcctggc
     9121 gcacacgtac cgggtctgca ccggcttttg aagcaaacag cagagcttca gagaccgctt
     9181 caatattgag cgctacgata atctgatttg ccactttgca ggtttgaccg tcgccgttac
     9241 cgcccacaag ggtgatattt ttaccgagca gttcaaacag cggtttaaca cgttcaaata
     9301 ccgcttcgtc accgccaacc ataattgaca acgtcccttc acgcgcgccg atttcgccgc
     9361 cggagactgg cgcatcgaga taatcaccac ccagttcatt cacctgacga gcgaaacgtt
     9421 tagtttcaat cggtgaaatg gagctcatat caacaatggt tttgcccttc agcgaggctt
     9481 tggtacaacc attttcacca aacagaactt cttcaacctg aggtgtgtcc ggcaccataa
     9541 taaaaatgat gtccgctgct tccgttacct ggcgcgcggt gtcgacatta acagcaccga
     9601 gtgacagtaa ttcatcagca accggtccaa tggtcgtgac atgtaattga tgaccggcac
     9661 gcgccagatt aatggccatc ggagtaccca taatgcctaa gccaataaat cccagtttca
     9721 taattaacct ctttttaaat gtcgctattc ctgaattcag acaacacgat cccctgccaa
     9781 cttagcagcc taaaaaagca ttgtctgaat agcgttacgt tttaacggta cggatccatc
     9841 cagcgtaaac cggcttccgt ggtggtttgg ggtttatatt cacacccaac ccaaccgttg
     9901 tagtcagaat tttcgattac cttaaagaga taatcataat taatttctcc ggtccccggt
     9961 tcgccgcgat gcggattatc ggcaatttgc aggtgaccaa ttttatcagc ccattg
//
LOCUS       DBFIFS010000161         9905 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.161, whole genome shotgun sequence.
ACCESSION   DBFIFS010000161 DBFIFS010000000
VERSION     DBFIFS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.161"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(49..1389)
                     /gene="gudD"
                     /locus_tag="N7A07_003510"
     CDS             complement(49..1389)
                     /gene="gudD"
                     /locus_tag="N7A07_003510"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU2478106.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(1410..2750)
                     /locus_tag="N7A07_003511"
                     /pseudo
     CDS             complement(1410..2750)
                     /locus_tag="N7A07_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(2752..4104)
                     /gene="gudP"
                     /locus_tag="N7A07_003512"
     CDS             complement(2752..4104)
                     /gene="gudP"
                     /locus_tag="N7A07_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU2478107.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(4539..4988)
                     /locus_tag="N7A07_003513"
     CDS             complement(4539..4988)
                     /locus_tag="N7A07_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU2478108.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(5006..5788)
                     /gene="truC"
                     /locus_tag="N7A07_003514"
     CDS             complement(5006..5788)
                     /gene="truC"
                     /locus_tag="N7A07_003514"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU2478109.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(5788..6117)
                     /locus_tag="N7A07_003515"
     CDS             complement(5788..6117)
                     /locus_tag="N7A07_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU2478110.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDYPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(6739..7284)
                     /gene="syd"
                     /locus_tag="N7A07_003516"
     CDS             complement(6739..7284)
                     /gene="syd"
                     /locus_tag="N7A07_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU2478111.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            7352..8200
                     /gene="queF"
                     /locus_tag="N7A07_003517"
     CDS             7352..8200
                     /gene="queF"
                     /locus_tag="N7A07_003517"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU2478112.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8312..9676
                     /gene="ppnN"
                     /locus_tag="N7A07_003518"
     CDS             8312..9676
                     /gene="ppnN"
                     /locus_tag="N7A07_003518"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU2478113.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         2468 a         2699 c         2328 g         2410 t
ORIGIN      
        1 catcagtctc aaaccggctc cagatagagc cggttttggt tttttgtctt aacgcaccat
       61 gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc
      121 gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat
      181 atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc
      241 aaacggttct ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc
      301 agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa
      361 gtggttgtta gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg
      421 taccgaacct tgcattgtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa
      481 cgagagcgta tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag
      541 gccagtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc
      601 acacggatct tctgcataag ccagcgaacc tttcaggtat ttgccgattt taatcgcttc
      661 gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc
      721 cagtgccaca atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa
      781 atcgttgaag ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt
      841 catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata
      901 cggcagaggt gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc
      961 gctacgctgt tgaccatcgc ccagcaggga tgccacgtta acccccagat gctgccccag
     1021 cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa
     1081 tgtctgcaaa ccgcgcccgc cggcatcacg atcggcaaaa gtattacgca ccagcgtcag
     1141 aacgtttttg tattcaccca gcgttttacc cactaccagc ggaattgcat cttccagcgt
     1201 tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt
     1261 gataatcaca atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat
     1321 actgtcatgg cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg
     1381 agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg
     1441 tcaaaggtcc agccggggat caggtactgc atcgggcctg cgtcgttacg cgcaccgcca
     1501 ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc
     1561 agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg attttgggtc
     1621 aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt
     1681 gccgccgcgc ccacatgggt aaacatcgcc agagagatat cgaaatggtt gttggaatgg
     1741 cagccccagg tcagccccca gtcgtcgcaa agctgcgcca ctcggactgc accggaaagc
     1801 gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc
     1861 atttcgcgcc agttggtggc gatcatatta gtcgcgacgg gcaagccggt cgcccgccga
     1921 aactccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca
     1981 taggtaagaa catcattcag ccctttgcac agagaaatgg cttcatcaag cagccatgca
     2041 ccgttgggat caacggtaat ccgcgcatcc gggaagcgtt tcttcaatgc acgaacagtg
     2101 tcgatttctt gctcgccagg taacacgccg cccttaagtt tgaaatcttt aaagccgtag
     2161 cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg
     2221 cgcaactgat accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg
     2281 gtccgatcac cgatataaaa cagataaccg aggacggtaa tagcatcgcg ttgcttgcct
     2341 ggccctaaca gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg
     2401 gcttccagcg ccgccacggc gttaacgcgc aattcaaagg tccaggcccc tttaccgaag
     2461 gtatcaaaat cggctgcttg attaccttta tgcacctgct gaaccacttt attcaggcgc
     2521 gcaacttcct agcccagcac catcggaata gcatcgacca atgtctgata aatcacctct
     2581 ccgcctggcg cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg
     2641 cgagtgaaat atgcgttatg cgccccacca atattaagca acatgctgtc gtacccggcg
     2701 accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc
     2761 cacgacaggt ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac
     2821 cgcgattaag gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc
     2881 aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa
     2941 caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc
     3001 cagcgcccca atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc
     3061 aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg
     3121 tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc
     3181 acccagcaca ccaccgataa aaccacaaac cgccggaacg gaagccacaa agcccgcttt
     3241 cagaatcgac atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta
     3301 agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag
     3361 cagttgtttg atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg
     3421 atccatattg atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacccc
     3481 cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatgg caatgcccag
     3541 accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat
     3601 cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc
     3661 cctttcctgc gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc
     3721 ttcagcaagc ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact
     3781 aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa
     3841 gtagacgcgt tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac
     3901 ataagcccat gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc
     3961 tttggccatt tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt
     4021 gacgataaac aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct
     4081 cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca
     4141 ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt
     4201 ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc
     4261 gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg
     4321 gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa
     4381 gggttaaaac gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc
     4441 cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc
     4501 agcaccgcta aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac
     4561 tgttcgaccc acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac
     4621 agcatttcgc caacccgctg agcgctctgt tcctgcagca aggcatcgaa ctgtttgccg
     4681 ccattgcaga aattcacata actactgtcg ccgagcgcaa tcacgccata acgcagattc
     4741 ggctggaagc ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg
     4801 tcgccctgcc cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag
     4861 tcgcttaatt caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct
     4921 tctgcttctt ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt
     4981 tccgccatac cgttttctcc ctggattagg aacttctctc tccatcctga cccgatggcg
     5041 cactgaactc aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg
     5101 cctgcatcca ggtgtcgtcc aggcccgcgt gaatagtcag cggctcacca gtaaaaggat
     5161 gcgtcaatga caactgactg gcatgcagca ttaaccgctg aagaccaaaa tgctcagcac
     5221 cgctgcgatt ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat
     5281 gggcaagatg tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc
     5341 cgtagcgcgt ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc
     5401 gataatgcgt cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt
     5461 tgtccagttc ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca
     5521 caatcgcatg gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc
     5581 cggcctcgct ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag
     5641 cagtaaaaac atgctggcct atctggtcac gcacggtttg catgaccact actttctcgt
     5701 cgcgatccag ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc
     5761 attcatcctg atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag
     5821 tttttccaac tcagccagaa taagcgcgcg ttgcggatag tccgtcgcca gcgccatttc
     5881 ataatagggt gcaacggcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg
     5941 cattcgcggg atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa
     6001 cggttgggta ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc
     6061 acgcagtaac gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa
     6121 gcaacctcaa taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt
     6181 attcacagtg ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca
     6241 tccttgacaa cttttcctct ggccatgcgg ccaatcgttc atcctgaact attgcttcct
     6301 gctcacacca ccccgatgtg atacttcatc ctgaagtgtc ccaggccttc ctgacccacc
     6361 gaatcatcct gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt
     6421 catcctcttc gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtccttcag
     6481 aagtgtcatc atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc
     6541 gtctttttcg cgaactctta caaggcgctt acagacaatg ccttaaggaa aatttcatat
     6601 gaaagttagg aattaacaag caacatgttg tttttaaatg aaaataacga ttcttatctt
     6661 tttaagtatt cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa
     6721 ccagatgggg tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt
     6781 tggggctaaa tgcgtccgtt tacgtgttcc cagcgtctct ttacacactt caccggagag
     6841 attacacacc gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggtgg
     6901 cagttttagc cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa
     6961 gtcgtcttca ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat
     7021 atccccggca aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa
     7081 agcgcgttca accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac
     7141 ggcatcttca gtagttgaaa taatgcacgg tgaaggaacg ccgtacagtt cctcacttaa
     7201 cggccagctt ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag
     7261 ggcttgtgcg gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc
     7321 gtttagttta tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact
     7381 tgcgggcctg actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact
     7441 gcaaggcgtt ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc
     7501 ttttcaaggt acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt
     7561 gccgcaggtc gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc
     7621 gaagagtttt aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga
     7681 ggtgcgccag acgctggagc gcgacttaag cacttgcgct cagggtgagg ttagcgtggc
     7741 gttatatcgt cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat
     7801 tgatgaccag gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc
     7861 caccagtggt gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg
     7921 cctgatcacc catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat
     7981 tgacagagaa aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga
     8041 acagtgcgtg gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag
     8101 cgtttacgca cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag
     8161 cgattttgtc ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt
     8221 gctggattca cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga
     8281 atgacgatat tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc
     8341 catggatatg ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct
     8401 ctatcaactg tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa
     8461 cagcaaagaa ttgctgtctc gttttgaaaa tttcgatatt aacgtcttgc gccgtgaacg
     8521 cggcgtaaag ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat
     8581 tcgcgctttg caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca
     8641 aatccataat accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa
     8701 cctggtcttt tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat
     8761 ggtggtctgc tggggcggtc actcaattaa cgaaaatgag tatttgtatg cccgtcgcgt
     8821 cggaaaccag ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat
     8881 ggaagcgccg atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg
     8941 ttttattggt atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa
     9001 cgaattgatc atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca
     9061 cggcatcatt atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg
     9121 aattttaatg aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa
     9181 agagagcgcc gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa
     9241 cgcgcgccgc cattaccgca taatcattga tgacgccgct gaagtcgccc gtcagatgaa
     9301 aaaatcgatg ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa
     9361 ctggtcaatg cgcattgcgc cagatttgca aatgccattt gagccgtctc acgagaatat
     9421 ggctaatctg aagctttacc cggatcaacc tgttgaagtg ctggctgccg atctgcgccg
     9481 tgcgttctcc ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga
     9541 gtttggtcct tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct
     9601 acagggtttt gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta
     9661 cgaaatctgc acgtaatctc ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt
     9721 tggtcactta ctcatcaact catttcattt gttatatgaa tgtttcttac caccctcacg
     9781 cgacaaatat catcacagtt aatatgtcat acaatttata ttgcaacgca aacgtttccc
     9841 tattttcata aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa
     9901 agtat
//
LOCUS       DBFIFS010000162         9903 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.162, whole genome shotgun sequence.
ACCESSION   DBFIFS010000162 DBFIFS010000000
VERSION     DBFIFS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.162"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            366..1343
                     /gene="glaH"
                     /locus_tag="N7A07_003519"
     CDS             366..1343
                     /gene="glaH"
                     /locus_tag="N7A07_003519"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU2478114.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1363..2631
                     /gene="lhgO"
                     /locus_tag="N7A07_003520"
     CDS             1363..2631
                     /gene="lhgO"
                     /locus_tag="N7A07_003520"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU2478115.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2654..4102
                     /gene="gabD"
                     /locus_tag="N7A07_003521"
     CDS             2654..4102
                     /gene="gabD"
                     /locus_tag="N7A07_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU2478116.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4116..5396
                     /gene="gabT"
                     /locus_tag="N7A07_003522"
     CDS             4116..5396
                     /gene="gabT"
                     /locus_tag="N7A07_003522"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU2478117.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5634..7034
                     /gene="gabP"
                     /locus_tag="N7A07_003523"
     CDS             5634..7034
                     /gene="gabP"
                     /locus_tag="N7A07_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU2478118.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7055..7717
                     /gene="csiR"
                     /locus_tag="N7A07_003524"
     CDS             7055..7717
                     /gene="csiR"
                     /locus_tag="N7A07_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU2478119.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7718..8167)
                     /gene="kbp"
                     /locus_tag="N7A07_003525"
     CDS             complement(7718..8167)
                     /gene="kbp"
                     /locus_tag="N7A07_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU2478120.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8251..8409)
                     /gene="yqaE"
                     /locus_tag="N7A07_003526"
     CDS             complement(8251..8409)
                     /gene="yqaE"
                     /locus_tag="N7A07_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU2478121.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8592..8891
                     /gene="ygaV"
                     /locus_tag="N7A07_003527"
     CDS             8592..8891
                     /gene="ygaV"
                     /locus_tag="N7A07_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU2478122.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8901..9419
                     /gene="ygaP"
                     /locus_tag="N7A07_003528"
     CDS             8901..9419
                     /gene="ygaP"
                     /locus_tag="N7A07_003528"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU2478123.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9472..9876)
                     /gene="stpA"
                     /locus_tag="N7A07_003529"
     CDS             complement(9472..9876)
                     /gene="stpA"
                     /locus_tag="N7A07_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU2478124.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2260 a         2541 c         2712 g         2390 t
ORIGIN      
        1 gagaaccgct gaactatctc tatttgtaat ttaatgttat atctgccccg ctaaaacggg
       61 gcagataata tatttagttt actagcggtc attttgcagt gaagccattt accgtttttt
      121 atcgaccaga taatctgttc tctgatggta atccccccct aacctgttgc tttagttatt
      181 catttcctgt ctcactttgc cttaataccc tacgttaaat gttaccaatt tgttgctttt
      241 tatcacaata agaaaacaat atggcgcatt tgtgcgcatt tttcagaaat gtagatattt
      301 ttagattatg gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc
      361 ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt cgattcagac caggactata
      421 gcggattcac tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg
      481 aacagacgac caaacagttt ctcgaacagg ttgccgaatg gcccgtgcag gcgctggagt
      541 acaaatcgtt tctgcgtttt cgggtaggta aaattcttga cgatctgtgt gcgaaccagc
      601 tgcaaccatt gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg
      661 cggtgggtgt cgatgatgtg aagcaagcgg atgagatggt gaagctggcg acggcggtgg
      721 cgcatctgat tggccgttcc aatttcgatg cgatgagcgg tcagtattac gcgcgttttg
      781 tagtgaaaaa tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtaatggagc
      841 tgcacaacga cggcacctac gtcgaagaga tcactgacta tgtgctgatg atgaaaatcg
      901 acgagcagaa catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc
      961 tggaccacta tttccgccat ccaatggcgc gtcgcccgat gcgctttgcc gcgccgccga
     1021 gcaaaaacgt cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc
     1081 cggtgatgcg ctatatcgac cagttcgtcc agccaaaaga cttcgaagaa ggcgtgtggt
     1141 tgagcgagct ttcggacgcc attgaaatca gtaaaggcat tctttctgta cccgttcccg
     1201 ttggcaaatt cctgttgatt aacaacctgt tctggctgca cggtcgcgac cgctttactc
     1261 cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc
     1321 accactatca gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt
     1381 attggcggcg gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ctatccggat
     1441 gcccgcattg cgttgctgga aaaagagtcc ggcccggcct gtcatcagac gggccacaac
     1501 agcggcgtga tccatgccgg ggtttattac acgcccggta gcctgaaggc gcagttttgc
     1561 ctggcgggaa accgcgccac taaagccttt tgcgatcaaa acggcattcg ctatgacaac
     1621 tgcggcaaga tgctggtcgc cacctctgaa ctcgaaatgg aacggatgcg cgcgttgtgg
     1681 gaacgcacgg cggcaaacgg tatcgagcgc gagtggttaa acgccgatga actgcgcgag
     1741 cgcgaaccga atatcaccgg gcttggcggt atttttgtgc cgtccagcgg cattgtcagc
     1801 taccgcgaag tcacggcggc gatggcaaaa attttccagg ccagaggcgg cgagattatc
     1861 tataacgccg aagtcagcgg ccttagtgag cataaaagcg gcgtggtgat acgtacccgt
     1921 cagggcagcg actatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg
     1981 ctggtgaaaa tgctcggcct cgaaccgggc tttattatct gcccgttccg cggcgagtat
     2041 ttccgcctgg cgccggagca taaccagatt gttaaccatc tgatttaccc cattcccgac
     2101 ccggcaatgc cgtttttggg cgtgcatctc acccgcatga tcgacggcag cgtcaccgtc
     2161 gggccaaacg cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt
     2221 agcgacacgc tggagatttt gggctcgtcg gggattcgcc gggtgctgca aaaccatcta
     2281 cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct gcggctggtg
     2341 caaaagtatt gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg
     2401 gcgcaggcgg tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccaccccg
     2461 cgcacgatcc acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt
     2521 gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg
     2581 ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcgt ttaatcaata acctttgaaa
     2641 acaggatgta gcgatgaaac tgaacgacag taacttattc cgccagcagg cgttgattaa
     2701 cggggaatgg ctggacgcca acaatggtga agtcatcgac gtcaccaatc cggcgaacgg
     2761 cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa actcgcgccg ctatcgacgc
     2821 cgccaaccgc gccctgcccg tctggcgtgc gctcaccgcc aaagaacgcg ccaacattct
     2881 gcgcaactgg ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct
     2941 cgaacagggt aaaccgctgg ctgaagcgaa aggcgaaatc agctacgccg cctcttttat
     3001 tgagtggttt gccgaagaag gcaaacgcat ttatggcgac accattcccg gtcatcaggc
     3061 cgataagcgc ctgattgtga tcaagcagcc gattggcgtt acagcggcca tcacgccgtg
     3121 gaactttccg gcggcaatga ttacccgcaa agccggtccg gcgctggcgg caggctgcac
     3181 catggtgctg aagcccgcca gtcagacgcc gttctctgcg ctggcgctgg cggagctggc
     3241 gatccgcgcg ggcattccgg ctggcgtgtt taacgtggtc accggttcgg cgggcgcggt
     3301 gggtaatgaa ctgaccagca acccgctggt acgcaaactg tcgtttaccg gctcgaccga
     3361 aattggccgc cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct
     3421 gggcggcaac gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg
     3481 cgcactggcc tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata
     3541 cgtgcaggac ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact
     3601 gcatatcggc gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc
     3661 ggtagcaaaa gtggaagagc atattgccga tgcgctggaa aaaggcgcgc gcgtagtttg
     3721 cggcggtaaa gcacatgaac gcggcggcaa cttcttccag cccaccattc tggtggacgt
     3781 gccggccaac gccaaagtgt cgaaagaaga gaccttcggc cccctcgccc cgctgttccg
     3841 ttttaaagat gaagccgatg tgatcgcgca agccaatgac accgagtttg gccttgccgc
     3901 ctatttctat gcccgtgatt taagtcgcgt cttccgcgtg ggcgaggcgc tggagtacgg
     3961 catcgtcggc atcaataccg ggattatttc caatgaagtg gccccgttcg gcggcattaa
     4021 agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa
     4081 atatatgtgc atcggtcttt aactggagaa cgcgaatgag cagcaataaa gagttaatgc
     4141 agcgccgcag tcaggcaatt cctcgtggcg ttgggcaaat tcaccccatt ttcgctgatc
     4201 gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg
     4261 gcattgcggt gctcaatacc gggcacctgc atccgaaagt ggttgccgcg gtggaagcgc
     4321 agttgaaaaa actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc
     4381 tgtgcgagat tatgaatcag aaggtgccgg gcgattttgc caagaaaacg ctgctggtta
     4441 cgaccggttc cgaagcggtg gaaaacgcgg tgaaaatcgc ccgcgctgcc accaaacgta
     4501 gcggcaccat cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga
     4561 ccggcaaggt gaatccgtac tctgcgggca tgggcctgat gccagggcac gtttatcgcg
     4621 cgctttatcc ttgcccactg cacggcatca gtgaagatga tgctatcgcc agtatccacc
     4681 gaatttttaa aaatgatgct gcgccggaag atatcgccgc catcgtgatt gagccggttc
     4741 agggcgaagg cggtttctac gccgcgacgc ctgcgtttat gcagcgttta cgcgcgctgt
     4801 gtgacgagca cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg
     4861 gcacgctgtt tgcgatggag caaatgggcg tggcaccaga tctcaccacc tttgcgaaat
     4921 cgatcgcagg cggcttccca ctggcgggcg tcaccgggcg cgccgaagtg atggatgccg
     4981 tcgctccagg cgggctgggt ggcacctatg ccggtaatcc gattgcctgc gtggcggcgc
     5041 tggaagtgtt gaaggtgttc gagcaggaaa atctgctgca gaaagccaac gatctggggc
     5101 agaagttgaa agatggattg ttggcgatcg ccgaaaaaca ccctgagatc ggcgacgtac
     5161 gcgggctggg ggcgatgatc gccatcgagc tgtttgaaga cggcgatcac aacaagccgg
     5221 acgccaaact caccgccgag atcgtggctc gcgcccgcga taaaggcctg attcttctct
     5281 cctgcggccc gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc
     5341 agatccgtca gggtctggag atcatcagcc agtgttttgc tgaggcaaag cagtagcgcc
     5401 gctcctatgc cggaggcgac gctgcgcgtc ttgtccggcc tacggggatc caggtcggat
     5461 aaggcgttta cgccgcatcc gacaatctgt acgtgaacag gaataaatct atgttggccg
     5521 ggtaaggcgg agccgctctc cggcaaaaag aatcaataac aattatacgc gtgacccgac
     5581 gcgggaaatg tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc
     5641 aatcatcgca accacatgag ttaggcggcg ggctgaagtc acgccatgtc accatgttgt
     5701 ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag
     5761 cgggcccggc ggtattactg gcctatctgt tcgccgggct actggtggtt atgattatgc
     5821 ggatgttggc ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg
     5881 ataaagctat tggtcgttgg gcgggttata ccatcggctg gttgtactgg tggttttggg
     5941 tactggttat cccgctggaa gccaacatcg ccgccatgat cctgcactca tgggttccag
     6001 gcattcccat ctggttattt tccctcgtca ttacccttgc cttaactggc agtaatttat
     6061 taagcgttaa aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta
     6121 tcctggcctt tattttcctt ggtgcagtcg caattagcgg tttttacccg tatgccgacg
     6181 tgagcgggat ctcaagattg tgggatagcg gcggctttat gcccaacggc ttcggtgcgg
     6241 tattaagcgc gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg
     6301 ccgccgcgga atccgacacg ccggaaaaac atattgtccg cgctaccaac tcggttatct
     6361 ggcgtatttc tatcttctat ttgtgctcta tttttgtcgt agtggcgtta ataccgtgga
     6421 atatgccggg gctgaaagcc gttggatctt atcgctcggt tctggaattg ctcaatatac
     6481 cccatgcgaa attaatcatg gactgcgtga tattactttc cgtaaccagt tgcctgaact
     6541 cggcgctgta taccgcgtca aggatgctct actccttaag ccgtcgcggt gatgcgcccg
     6601 cggtaatggg caaaatcaac cgcagtaaaa ccccgtatgt ggcggtgtta ctctccaccg
     6661 gagcggcatt tttaacggtc gtggtgaact attacgcacc tgcgaaagtg tttaaattcc
     6721 tgatcgacag ctccggtgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt
     6781 tgcggatgcg taaaattctg cgagcagaag gaagcgaaat tcgcttgcgc atgtggcttt
     6841 acccgtggct cacctggctg gtaatcggct ttattacctt tgtgttggtg gtgatgttat
     6901 tccgcccggc gcaacagtta gaagtgatct ctaccggctt attagcgata gggattatct
     6961 gtaccgtgcc gattatggcg cgctggaaaa agctggtatt gtggcaaaaa acacccgttc
     7021 ataatacgcg ctgataatga tcaggagtca caccatgacc attacgtctc tggatggcta
     7081 tcgctggctg aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacgaat
     7141 gagtttgctg acatcgcgtt atgcacttgg cgttgggccg ttacgggaag ctctttcgca
     7201 actggtggcg gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat
     7261 gtcagagcag gagctgctcg atattttcga cgcccgcgcc aatatggaag cgatgttagt
     7321 gagtctggcg attgcccgcg gtggcgatga gtgggaggca gacgttctcg caaaagcgca
     7381 tctgctgagt aagcttgagg cctgtgacgc cagcgagaaa atgcttgatg agtgggatct
     7441 gcgtcatcag gcgtttcata ctgcaattgt ggcgggctgt ggttctcact atttgctgca
     7501 aatgcgtgaa cggttgtttg atctggcggc gcgttatcga tttatctggc tgcggcgaac
     7561 ggtgctttcg gtggaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc
     7621 ggtactggcg cgagataccg cgcgcgccag tgagttaatg cgccagcatt tactgacgcc
     7681 aattcccatt atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca
     7741 cttgccccgg ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt
     7801 tattgtacag attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag
     7861 acttaacggt ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcaa
     7921 cactggcaat accggaaata ttccccaccg caacaaggat tttctccttc gcctcctgac
     7981 tcaggccgtc accagtgacc gtcgctttgc catcggcaat ttgaatgttc actttatcgg
     8041 catcaggtat accggttttg ctcagatgct cctgcacctt cttcgcctga tcatctttat
     8101 cgtgctgacc tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca
     8161 gacccatagc tacctcattc gttaacggaa acactcagaa agtgtagtag aggaacgcgg
     8221 cgcgttaacg ccgcggggat gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg
     8281 aatcaaaccg gggatatacc caagcagtgt caacagaata ttaataatga acgcccaacc
     8341 gaacccttta ccgagcagca cgccgagcgg cggcagaatg atggtgatga cgattctcca
     8401 gaaacccata tgtactccct ataagaaaat tacttattgt ttaaaaagag attttatctc
     8461 ttaagcgtag taattttagc ggaggctgcc agtttcagct tacgctttta ctctcattta
     8521 cgctgctgag gctggtatat gttgcatttt tagtttagag tttacttaat ttagaaaata
     8581 cttaaataac tatgaccgaa ctcacgcaat tacaggccag tgctgaacag gcagcggcct
     8641 tattgaaagc aatgagccac cctaaacggt tgctgattct gtgcatgctt agcggttccc
     8701 ccggcaccag cgcgggagag ctgacgcgca ttaccggact gagcgcctct gcgacatcac
     8761 agcatctcgc tcgtatgcgg gacgaagggc ttatcaacag ccaacgggat gcccaacgca
     8821 ttctatattc cattaaaaat gaggcggtaa atgccattat cgccaccctg aaaaatgtct
     8881 attgtccgta aggagtcact atggctttga caaccatttc gccgcatgat gcacaagaat
     8941 taatcgcacg cggcgcaaag ttaatcgata ttcgtgacgc tgatgaatat cttcgtgaac
     9001 atattcctga agcagatctg gctccattat ccgtgctgga acagtcaggt ctcccggcta
     9061 aattacgtcg cgatcaaatt atattccact gccaggcagg taaacgcacc agtaataacg
     9121 ccgataaatt agcagcgatt gccgcccccg cagaaatctt tttactcgaa gatgggattg
     9181 atggctggaa aaaagcagga ttaccagtag cggtaaataa atctcaaccc ttgccgttaa
     9241 tgcgccaggt gcagatcgct gcgggtggtt taatattaat cggcgttata ctgggttata
     9301 ccgtaaatag cggtttcttc ttattaagcg gctttgttgg tgccgggtta ctgtttgcag
     9361 gaattagcgg tttttgtggg atggcaaggt tgttagataa gatgccgtgg aaccaatgag
     9421 cttgagaagc gacgccggac gcgccctagc agcgacatcc ggcctcagta attagatcag
     9481 gaaatcgtcg agagatttac cttctgccag cgcctgagcg attggcttcg gtgtacggcc
     9541 ctgaccggtc caggttttag tttcaccgtt aacatcggtg aatttatatt tcgccggacg
     9601 cggctggcgt tttttaccag cacgtggtgc agcagcagag ctattaccca ataactcttc
     9661 cgggttaatt ccgtcagctt tcatcagctc cagccaggtg ctaatttttt cctggcgctc
     9721 tgccagttca cgctgctgct gttcttcttc ttcacgtctt tctttagtga caaccctgaa
     9781 tttttcgagc atttcttcaa gaacgtcaat ggagaattcg cgagccatcg cacggagggt
     9841 gcgaatgtta tttaaacttt gtaacattac ggacataata aagaaaacct tttaacgcca
     9901 aaa
//
LOCUS       DBFIFS010000163         9874 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.163, whole genome shotgun sequence.
ACCESSION   DBFIFS010000163 DBFIFS010000000
VERSION     DBFIFS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.163"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..162)
                     /locus_tag="N7A07_003530"
     CDS             complement(<1..162)
                     /locus_tag="N7A07_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478125.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLI"
     gene            complement(210..1091)
                     /locus_tag="N7A07_003531"
     CDS             complement(210..1091)
                     /locus_tag="N7A07_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGU2478126.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(1188..1352)
                     /gene="yjiS"
                     /locus_tag="N7A07_003532"
     CDS             complement(1188..1352)
                     /gene="yjiS"
                     /locus_tag="N7A07_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU2478127.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            1529..2941
                     /gene="yjiR"
                     /locus_tag="N7A07_003533"
     CDS             1529..2941
                     /gene="yjiR"
                     /locus_tag="N7A07_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2478128.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(3184..4141)
                     /locus_tag="N7A07_003534"
                     /pseudo
     CDS             complement(3184..4141)
                     /locus_tag="N7A07_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            4608..5840
                     /gene="mdtM"
                     /locus_tag="N7A07_003535"
     CDS             4608..5840
                     /gene="mdtM"
                     /locus_tag="N7A07_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU2478129.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5881..7152
                     /gene="yjiN"
                     /locus_tag="N7A07_003536"
     CDS             5881..7152
                     /gene="yjiN"
                     /locus_tag="N7A07_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU2478130.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            7268..8419
                     /gene="yjiM"
                     /locus_tag="N7A07_003537"
     CDS             7268..8419
                     /gene="yjiM"
                     /locus_tag="N7A07_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU2478131.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVVETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            8429..9196
                     /gene="yjiL"
                     /locus_tag="N7A07_003538"
     CDS             8429..9196
                     /gene="yjiL"
                     /locus_tag="N7A07_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU2478132.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            9193..9459
                     /locus_tag="N7A07_003539"
     CDS             9193..9459
                     /locus_tag="N7A07_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU2478133.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         2318 a         2480 c         2489 g         2587 t
ORIGIN      
        1 aattaaacga attatgctca ggaaatcttc gtgagacaaa tcgtcatttt tatagtcatg
       61 ataaagcgcc agtaagaaag gatacgccac atcaaccttt aactcgcgca aatcctgaaa
      121 agccgtagca agcgatttgt cactttcttt tcccaatgcc attgcacagt aatactccgc
      181 gtagatatga atatcttcaa ccagccgatc tacgcctttt tcagcaacac tctggctgcg
      241 ggcatggagt ttaaatgcct catagacttc atctgtccga gggatctccc cgtttttacc
      301 gtcagataat gacgcataaa actgtcaaaa tattcgctgt aaccttgctg accacaagcg
      361 acttccattg gacgccagtg atcttcatac aaccgggttt gatgctctgg ttcgaggccc
      421 atcagaataa aattgcgcac cagatcggcc tgacttaacg ccttaccggt ggagttcata
      481 ctttcaaaaa tcagttgcgg attatcctga ccacgattaa gcgccacatc gacaattaat
      541 aactttgcta acccacgaca taaggggatc aagtcatcac cgagtttggc aatttgttca
      601 tcaaagaaag taaagttttc cattattcga tgggaatagt tttctggcat tggtctttgt
      661 tttattaacg ccagtaaact gtctttatca gtctcggtca gcagtaattt aaaacctttc
      721 tcgccggact cataaggatt gagcaaataa taattacgca atttcattgc cgaaaagcca
      781 tcaaaaactt cgtcttcgcc aagatggcgc gataaagcct caatcagcaa cattgcggtc
      841 gtcagccgct gctgaccatc aatgaccagc aacggagaaa taccagaaac ctgatacaat
      901 ccctgctcta tataaacaac cgaaccgata aaatgcgctg atatatcgtc acgctttcct
      961 gcacgaatga tgtcgtccca aagttgccga cattgttgtt ctgtccatga ataagtccgt
     1021 tgataaatgg gaataacaaa ctgctgcgaa cgttttaaaa aatcgagtag gcgagcttct
     1081 gtagctttca ttctgacgtc tttccgtgaa acgatgattt cgcaataata accacttcct
     1141 tatggaggtt atagagaaat cctgtttatc cgcgacgaac atacccctca ctccacatcc
     1201 tccctgcgta acccaatatc cctcaaccgt tcatcactca tctgctgtaa cacccgtcgg
     1261 gcctgcattt gccgccgcca acgcctcact gcctgccaga gttgcacaag tctgataaac
     1321 ggcgttttgg ctttgttttc gtgaaattcc atctgctgcc tcctcatatt tcagaggctt
     1381 catcatccgt cttctggcag ttcacaatac agatgcaaat attagttttg tttaacatac
     1441 agacaggtta aaacagcatc tgcatccggt tttttagcct catctgtacc ggtttttctt
     1501 tctgtatggt ccaaataagg aatacagcat gacgcgttat caacatctgg cgactctact
     1561 tgccgagcgg attgagcaag ggctgtatcg tcacggggag aaattgccgt cggtgcgcag
     1621 cttaagtcag gagcacggcg tcagcatcag caccgtgcag caggcgtacc agacgctgga
     1681 gacgatgaag ctcatcactc cgcagccgcg ttcgggttat tttgtcgcac aacgtaaagc
     1741 ccagccgcca gtgccgccga tgacgcgtcc ggtgcagcgc ccagtggaaa ttacccagtg
     1801 ggatctggtg ctggatatgc tggtggcgca tagcgacagt tccattgttc cgttaagcaa
     1861 aagcacgccg gatgtcgaaa cgcccagcct gaaaccgctg tggcgggagc taagccgggt
     1921 ggtgcagcat aatctgcaaa ccgttctcgg ttatgacttg ctagccggtc agcgggtatt
     1981 gcgcgagcag gttgcccgcc tgatgctcga cagcggctcg gtggtcactg ccgatgacat
     2041 catcatcacc agcggctgcc ataactcgat gtcgctagcg ttaatggcgg tgtgtaaacc
     2101 gggcgatatt gtcgcggtcg aatccccctg ttattacggt tcaatgcaga tgctgcgcgg
     2161 catgggcgtg aaagtgattg aaatcccaac cgatccagaa acaggcatca gcgttgaagc
     2221 gctggaactg gcgctggaac agtggccgat taaaggcatc attctggtgc caaactgtaa
     2281 taatccgctg ggatttatta tgccggacgc gcgcaaacgg gccgttctct ctctcgctca
     2341 gcgtcatgat attgtgattt ttgaagatga tgtctacggc gaactggcaa cggagtatcc
     2401 gcgcccgcgg accattcatt cctgggatat cgacgggcga gtgctgttgt gcagctcgtt
     2461 cagtaaaagt attgctccag gcctgcgcgt gggttgggtc gcaccggggc gttatcacga
     2521 taaactgatg catatgaaat acgccatcag cagctttaat gtgccgtcca cgcaaatggc
     2581 ggcggcaacg tttgtgctgg aaggtcacta tcatcgccat atccggcgga tgcggcagat
     2641 ctatcagcgc aatttggcgc tttatacctg ctggatacgg gaatattttc cctgcgaaat
     2701 ctgtattacg cgcccgaaag gcggattttt actgtggata gaattgcctg aacaggtcga
     2761 tatggtctgc gtcacgcggc agctgtgccg catgaaaatc caggtggcgg caggctcgat
     2821 tttctcagct tccggcaaat accgtaactg cctgcgcatc aactgtgctt tgccactcag
     2881 cgaaacttat cgcgaagcgc tgaagcaaat tggcgaggcc gtgtatcggg caatggaata
     2941 aattagaatt agtggcgaag cacctgattt attctcacga atcgcctgtg actcgtttta
     3001 tctttgcagg atcatagcca cgttccggca tggcttaggt aaagtgcgct ttgttcatgc
     3061 cggatgccat gtgaacgcct taccaggtct acaaaatctt taaagttcaa tatattgaat
     3121 gggctttgta gacctgataa gcgtagcaca tcaggctgct ttgcgtttgt catccgtctc
     3181 tggttagctc ttgagccatg aatagcgctc acgctcaggc aagggctgcc ttaatgcctg
     3241 catttgctct gccgaaagtc cggtaatctt ctgtatccat tcaggatctg ccccattctg
     3301 caacaacaat cgaagaatgc gctgttcccc ttcttccctt cccaacagcc atccatcatt
     3361 atgaattcgc tctgcaatcg tcattatgaa ttcgctctgc aatcgtcatt attcgctctc
     3421 tgtgttgagg cattcgactg gttagttcac tgataaactc attaaaacgc tcttcatcgc
     3481 cagtcagtaa aatgtaattt aacagcgcag ttatctggct gtcattagca cactctgtaa
     3541 ccagtaatac taccagttga tcgataagcc ccatcagatc gcgctggcga atatgctttt
     3601 ggatcaactc caacagggcg actctgcggt gctgcacaat ctcgtcgtcc ggcacgacag
     3661 tgatatccac cagcgggaac gcggcgttat aaagcttccg tgcagtagtc gggtcggcaa
     3721 attcgtccaa ccagcacaaa gaccagggat aaggactacg gctaccgtga taaaacagca
     3781 tcgggatgac cagcggtagt ggccggcgtt tatcatgctc tatatggcgc tgcatcactg
     3841 ccatggaata gcgcatcagg cgaaaggcca tatgaatatc ctcacggctc tgatgttcaa
     3901 tcaccacata gatatagcca tcgccttccc gggtctttac cgaccacaga atatcggagt
     3961 gtagcgcccg caatttttca tcgacaaagc tggcggattc cagttttaag ctgtcgagat
     4021 cgcacagttc acgtaaatct ttgggtaagt ggatctccat aaaatcccgc gcggtgtcag
     4081 ggtgcatgag aaaagattta aataatgcgt catgcggcgt gctggtcgtg aagtttgtca
     4141 tggcgatccg tcacctgttg ataagatggc ccgactttag cgactaacaa aaccattctc
     4201 acccggcaat ttctcgtttc ccgaggcgct ttccaggcta tttttgttcg tctgcaaacg
     4261 atacattttt ccggaaactg actcgcgttt tacatgtcct gtacccggca gatattcccc
     4321 cttccaccgc gctcttttga ttaatcgcaa attttaatta cacttaaggt gtatattttc
     4381 tatgcaaccc atcaattcaa gaggtgtaat gtgctgatga ctatttgtaa tcgttataca
     4441 ttctgacccg aagtcagaag gtatttctct gtctgtgtgt tcacaggcag tgtggttgat
     4501 tacatgaaat tcagtacatt tgtccttctc gcctaccttt cgtcattacc gacggtattg
     4561 aatttcgttt tccccgttgg ggttctccgg acaaggagtt gtttgttatg ccacgttttt
     4621 ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt gattttgtat gactttgccg
     4681 cctatctttc gacggacctg atccagcctg ggatcattaa tgtggtccgt gattttaatg
     4741 ccgatgtcag tctggctccg gctgccgtca gtctctatct cgctggaggc atggcgttgc
     4801 agtggctgct ggggccgctt tccgacagga ttggccgcaa gccagtgctg attaccgggg
     4861 cgctaatatt tacccttgcc tgcgctgcga caatgttcac aacgtctatg acacagtttc
     4921 ttatcgcgcg tgcaattcag ggcaccagta tctgttttat tgccaccgtt ggttatgtca
     4981 cggtgcagga ggcgttcggc cagacaaaag ggatcaagtt gatggcgatt atcacctcca
     5041 tcgtactgat tgcgccgatt atcggcccgc tttccggcgc agctctgatg cactttgtgc
     5101 actggaaagt cctttttgcc atcattgcgg ttatgggttt tatctcattt gttggcttac
     5161 tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc atttagcgcc aaaagcgtct
     5221 tgcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct ctttggcgca gcaaccatct
     5281 ctttaagcta tatccccatg atgagttggg tggctgtctc gccggtgatc ctgattgacg
     5341 caggcggctt aacgacctcg cagttcgcct ggacacaggt tccggtgttc ggcgcggtga
     5401 ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc gaccgaaccg cgctttatct
     5461 ggcgtgccgt acccattcaa ctggtcggcc tcgcgctgtt gattatcggc aatctgctgt
     5521 caccgcacgt ctggctgtgg tcggtgctgg gcaccagtct gtatgctttc gggattggtt
     5581 tgatcttccc gaccttattc cgctttacgc tgttttccaa taacttaccg aaagggaccg
     5641 tctccgcatc gctgaatatg gtgatcctga tggtgatgtc tgtctcggtc gaaattggcc
     5701 gctggctgtg gtttaacggc ggtcgcttgc cgtttcatct gttagccgtt gtggcgggcg
     5761 ttatcgtcgt tttcaccctg gccggattgc ttaatcgcgt gcgccaacat caggctgccg
     5821 agctggcaga ggagcagtga tttttgcgcg atcctgccgt caggctctat tcttaacgtt
     5881 atgaataaac tcattgaact cagacgcgcc aaaatgttgg cgctctcttt actgcttatc
     5941 gccgctgcta cctttgtcgt tacgctgttt ttgccgccca atttttgggt gagcggcgtg
     6001 aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg actggtttgc ggtggtggcg
     6061 ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata cggcgattat cccgcgtaat
     6121 aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcaga aaaaatttct cgatacccaa
     6181 tcgctggtgg cattgattcg acgtcacgaa ccggcgttgc tgattggcaa ctggtttagc
     6241 cagccagaaa acgcccgccg cgttggtcag catctgttgc agatcatgag tggttttctt
     6301 gaactgaccg atgatgcacg tattcagcgc ctgcttaagc gcgcggtcca tcgggcgatt
     6361 gataaggtcg atctttccgg caccagtgcg ttgatgctgg agagtatgac caaaaacgat
     6421 cgtcatcagg tgctgctgga tacgctgatc gcacagttga tcgcccttct ccagcgcgat
     6481 aaatcgcgca agtttatcgc ccagcagatt gttcgctggc tggagagtga gcatccactg
     6541 aaagccaaaa ttctccccac cgaatggctg ggcgaacata gcgcggagtt ggtttctgac
     6601 gcggtgaatt ctttgcttga tgatatcagc cgcgatcgtg cgcatcagat ccgccatgca
     6661 tttgatcgcg ccacttttgc cctgatcgac aagttgaaaa acgatcctga aatgacagcg
     6721 cgagctgatg ccgtaaaaag ctatctgaaa gaggatgaag cctttctcag tgaattgtgg
     6781 ggggatttac gggaatggct gaaagcggat atcaacagtg aagattctcg tgtgaaagaa
     6841 cgtatcgcgc gggcaggtca atggtttggc gaaacgttaa ttgccgatga tgccttgcgg
     6901 gcgtcgttaa atggtcacct ggaacaagcc gcgcaccgcg tcgcgcctga gttttctgca
     6961 ttcctgacgc gccatatcag cgatacggta aaaagctggg atgcccggga tatgtcgcgg
     7021 caaatagagt taaatatcgg caaagatctg cagtttatcc gtgtcaacgg tacgctggtt
     7081 ggcggttgta ttgggctaat tttatatttg ctgtcacagc tcccggcctt gttccccctc
     7141 ggcaattttt agaaatccat gaaaaactaa gtggctaacc gcgagagaga tcaaataaac
     7201 catactaaaa gaatggtaat aatgtggcct cttttcatta tctcccgtgg tacggggaag
     7261 gaaaatcatg tcacttgtca ccgatctacc cgccattttc gatcagttct ctgaagctcg
     7321 ccagaaaggc tttctcaccg tcatggatct caaggtgcgc ggcattccgc tggttggcac
     7381 ttactgcacc tttatgccgc aagagatccc gatggcagcc ggtgcggttg tggtttcgct
     7441 ctgttccacc tctgatgaaa ccattgaaga agcggagaaa gatctgccgc gcaacctctg
     7501 cccgctgatt aaaagtagct acggcttcgg caaaaccgat aaatgcccct acttctactt
     7561 ttcggatctg gtggtcggtg aaaccacctg cgacggcaaa aagaaaatgt atgaatacat
     7621 ggcggagttt aagcccgttc atgtgatgca gctgccaaac agcgttaaag acgatgcctc
     7681 gcgtgcgtta tggaaagccg agatgctgcg cttacaaaaa gcggtggaag aacgttttgg
     7741 gcacgaaatt agcgaagatg ctctgcgcga tgccattgcg ctgaaaaacc gcgaacgtcg
     7801 cgcactggcc aatttttatc atcttgggca gttcaatcct ccggcgctta gcggcagcga
     7861 cattctgaaa gtggtttacg gcgcaacctt ccggttcgat aaagaggcgt tgatcaatga
     7921 actggacgcg atgaccgccc gcattcgtca gcagtgggaa gaaggccagc gactggaccc
     7981 gcgtccgcgc attttaatca ccggctgccc gattggcggc gcagcagaga aagtggtgcg
     8041 cgcgattgaa gagaatggcg gctgggttgt cggttatgaa aactgcaccg gggcgaaagc
     8101 gaccgagcaa tgcgtggtag aaacgggcga tgtctacgac gcgctggcgg ataaatatct
     8161 ggcgattggc tgctcctgtg tttcgccgaa cgatcagcgc ctgaaaatgc tcagccagat
     8221 ggtggaagaa tatcaggtcg atggcgtagt tgatgtgatt ttgcaggcgt gccataccta
     8281 cgcggtggaa tcgctggcaa ttaaacgtca tgtgcgtcag cagcacaaca ttccttatat
     8341 cgctattgaa acagactact ccacctcgga tgttgggcag ctcagtaccc gtgtcgcggc
     8401 ctttattgag atgctgtaag gagtagcagt gacatattcg attggcattg attctggttc
     8461 taccgccacc aaagggatct tactcgcaga cggcgtgatt acgcgccgtt tcctcgttcc
     8521 aacacccttt cgaccggcaa cagcaattac tgaagcctgg gaaactctgc gcgaagggtt
     8581 agagacaacg ccgtttctga cgctcaccgg ctacgggcgg caactggtgg attttgccga
     8641 taaacaagtg acggaaatct cctgtcacgg gctgggcgca cggtttcttg cgccagcaac
     8701 gcgcgcggta atcgacatcg gtggtcagga cagcaaagtg attcagcttg atgatgacgg
     8761 taacctgtgc gatttcctga tgaatgacaa atgcgcggcg ggcaccgggc gtttcctgga
     8821 ggtgatctcg cgcacgcttg gcaccagcgt cgagcaactc gactgcatta ccgaaaatgt
     8881 cacgccgcac gccatcacga gtatgtgcac agtgtttgct gaatcagaag tgatcagcct
     8941 gcgctcagcg ggcgtcgcgc cagaagcgat tctcgccgga gtgattaacg cgatggcgcg
     9001 gcggagtgcc aatttcattg ctcgtctctc ctgtgaagcg ccgattctgt ttactggtgg
     9061 cgttagtcat tgccagacgt ttgcccggat gctggaatct cacctgcgaa tgccggtaaa
     9121 tacccatcct gatgcgcaat ttgctggcac aattggcgcg gcggtaattg gtcaacgagt
     9181 gaggacacgc cgatgaaaga gtttttattt ttatttcact ccacggtcgg cgtcatacaa
     9241 acccgcaagg cgttgcaggc agcgggcatg acttttcgcg tcagcgatat tccccgtgat
     9301 ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc cccccggcga ggaaatacaa
     9361 tgggtgatcc ctgggcaaac cgaagcgata tattgccagc agggcagcga ctggcagtgc
     9421 gtggtgcatt acgatgcaga agcgtcaacc aggcaatagg atctttaaat ataccaaccg
     9481 gataattggc tgcgcataat cctgcatgaa ggaagttgta gtaaatatct cgcctgccgt
     9541 gaaatatttc ccatccacaa aatgagcgat ttttgatagc accttcctat tgcgcgtcta
     9601 aaatacaata tacattgaat tatacttaaa cccataatca tgcatagaga aataaaatga
     9661 ttaccgggtg ttgtaatgtt aaaagataag tttctgtgca aagcttatct ttccccaccc
     9721 cctgattatt ttagatttag aagaatattt cacagttcaa ggataaaaat atgggctccc
     9781 tatttaacat atacaaagat attttcccaa cactcggcat gtattcggga ctaaaagctt
     9841 gccatgaaaa aaacaaccta ccatttgata ttaa
//
LOCUS       DBFIFS010000164         9873 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.164, whole genome shotgun sequence.
ACCESSION   DBFIFS010000164 DBFIFS010000000
VERSION     DBFIFS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.164"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..76)
                     /locus_tag="N7A07_003540"
     CDS             complement(<1..76)
                     /locus_tag="N7A07_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478134.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNS"
     gene            complement(204..2165)
                     /gene="topB"
                     /locus_tag="N7A07_003541"
     CDS             complement(204..2165)
                     /gene="topB"
                     /locus_tag="N7A07_003541"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU2478135.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2170..3213)
                     /gene="selD"
                     /locus_tag="N7A07_003542"
     CDS             complement(2170..3213)
                     /gene="selD"
                     /locus_tag="N7A07_003542"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU2478136.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3330..3881)
                     /gene="ydjA"
                     /locus_tag="N7A07_003543"
     CDS             complement(3330..3881)
                     /gene="ydjA"
                     /locus_tag="N7A07_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU2478137.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4042..5898
                     /gene="sppA"
                     /locus_tag="N7A07_003544"
     CDS             4042..5898
                     /gene="sppA"
                     /locus_tag="N7A07_003544"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU2478138.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6014..6724
                     /locus_tag="N7A07_003545"
     CDS             6014..6724
                     /locus_tag="N7A07_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HGU2478139.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            6856..7872
                     /gene="ansA"
                     /locus_tag="N7A07_003546"
     CDS             6856..7872
                     /gene="ansA"
                     /locus_tag="N7A07_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU2478140.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7883..8524
                     /gene="pncA"
                     /locus_tag="N7A07_003547"
     CDS             7883..8524
                     /gene="pncA"
                     /locus_tag="N7A07_003547"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU2478141.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSALFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8617..>9873)
                     /gene="ydjE"
                     /locus_tag="N7A07_003548"
     CDS             complement(8617..>9873)
                     /gene="ydjE"
                     /locus_tag="N7A07_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2478142.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         2451 a         2625 c         2446 g         2351 t
ORIGIN      
        1 tactattaaa tatttcccag ccattaaaag aatacttctc gctcccagga tggttttgta
       61 ataaaacttt tttcatcaca ttccctgtta ttacatactt tagaggcgat aattttatcc
      121 atgcaaaaaa aatatccaac gaattttctt gatccgctgg atatttcaga atatgactcg
      181 aatagcacga aagattcgct cgcttacgct atcgccccgc ttccggcttc atctgctggc
      241 ggactttttt tcgcactacg tttacgcggt gcagcctttt tcttatcagc actgccacca
      301 ctgcccggag ccacaatgcc gcgaaactgc cgcaccggag tacgtttggc ttgatcaata
      361 agctgatata gcgtccccac cagcggctgc ataaagtcct gatagcgaca ctgcttttcg
      421 ctgatttgcg tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt agccatttct
      481 ggcagcgaat ggaataacgc ttttccggcg tcggtggagt ggatatagcg cccttttttg
      541 atgaggaaac cacgcttgaa caacagttca ataatcccgg cacgcgttgc ttctgtcccc
      601 agaccatcgg tcgcacgaag gatctttttc agatctttgt cctgcacaaa gcgcgcgatc
      661 ccggtcatcg ccgaaagcag tgttgcatcg gtaaaatggc gcggcggctg ggtttgccgc
      721 tctaccactt cacctttttc acacagcaac tcatcgcctt tcgccaccac aggcaatggc
      781 gtaccgtcgt tttcttcatc gcgctctttg ctgcctaaca gcgtgcgcca gcctgcttca
      841 gcaagaaaac gcgctttagc gacaaatttg cctttggcaa tgtccagttc gataacacac
      901 ttgcggaaca ccgcatccgg gcagaactgc atcagatact gacgggaaat caggttatag
      961 accttcgctt cgttctccgt caggttgatc gcagaactcc gtgcggtcgg aatgatggcg
     1021 tggtgcgcat cgaccttttt gtcatcccaa cagcggttgc gtatatctgg atctaccact
     1081 ggctgcggca acagatctgg tgcatgaacg ctgatggcat tcatcaccgc gtggcgtccg
     1141 gcaaaatgtt cttctggcaa atagcgacaa tcggaacgcg gataagtgat tagcttgtgc
     1201 gtttcgtaca gcttctggca gatatcaagc acgttctgcg cgctcagacc aaagcgtttt
     1261 gccgcttcaa tctgcaacgc cgaaagcgaa aacggcagcg gcgcggattc tgattcccgt
     1321 ttatcgttat agctggtgac aatagccggt tgaccactaa tgcggttaac cacatgctcc
     1381 gccagtggac gatgtaacaa gcgcccttct tcatcctggt acggttcaca cgcttcgctc
     1441 ggttgccaga tagcggtaaa ccgctcatcg gcaggtgtca cgatatgtgc tttgacttca
     1501 aagaagtctt tcgccacgaa gttttcaatc tcttcatcgc ggcgcaccac cagcccaagc
     1561 acgggcgtct gcacgcgtcc cacggaaagc acgccctgat aaccggcatt gcgaccgaga
     1621 atggtatacg cacgggtcat attgatgccg tacagccagt cggcacgcgc tcgcgccagc
     1681 gcagaaacgc acagcggtac aaactcactg ttggaacgaa gacggtcgat cgcccgctca
     1741 accgcctgcg ggttcaggtc gtttatcaag caacgctgca cctgctggcg cttttccggt
     1801 gccagttgta gatagtccag cacttcatcc accagcagtt gcccttcgcg atccgggtcc
     1861 ccggcgtgaa cgatttcgct ggcttcatgc aggtaccgtt tgatgacgtt aagttgtttg
     1921 gtcacggagg gtcggggctg taattgccac ttttccggga caatcggcaa atccgcaaga
     1981 ttccagcgcg catagcggct gtcgtaggcg tctggctgcg cctgctcaag caggtgaccg
     2041 atacaccagg tcaccacctg accattaccg cactcgataa agccatcgcc tttccggtgc
     2101 ggtttgggca ggacatcagc aatggcgcgc gccagactcg gtttttcggc aataaacaac
     2161 cgcattgaat taacggatct caaccatggc acgaccgccg cgtgctggca ccagttcgcc
     2221 aatcgccgtc agttcaatgc caaactcggc ggctgtagct ttgacctcat tttcggcttc
     2281 cggcatgacc gccagcagca aaccgccaga agtttgcgga tcgcacagca aatcgcgcac
     2341 ttcacgcggc atttcaccca tcagatgacc gtagctggca aagttacgtt cggtgccgcc
     2401 aggcactgca cccaacttaa tgtactcttc aacccccggc aatttcggga tcgcgtcata
     2461 gtcaacgcgc gcctgcacac ctgcgccctg acacatttcg ctcaaatggc ccagcaggcc
     2521 aaagcccgtt acgtcggtca tcgcttttac gccttcaatg ttggcaaagg acgcgcctgc
     2581 gatgttcatc cggcacatca cttccgtcgc cagtccctga tgttctggtt tcagtagtga
     2641 ttttttctca gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga acagtttaca
     2701 tccggcttgt gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc ccgttaccgc
     2761 cagaccaaaa atcggctccg gcgcatcgat ggagtgaccg ccagccagcg caatacccgc
     2821 ctgacggcat gcatagcgtc caccttcggt tacttcgcgg gcaatttccg gggaaagttt
     2881 gttaatcggc cagccgagga tcgcaattgc cataatcggt ttgcccccca tcgcgaagat
     2941 atcgctgatg gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat caacgatcgg
     3001 cataaagaag tcggtggtac tgataacgct ggtgccattg cccagatcgt acaccgccgc
     3061 atcgtcgcgg gtttcattac ccacaagcaa attcggatca acaaacttcg cctgctcact
     3121 gtgcaggatg gtttccaaca cttttgggga aattttacag ccgcaaccag ctccgtggct
     3181 gtattgggtc aaacgaatcg agttctcgct catggacatc tcctgtcaat gcaatccggg
     3241 tatggtaacc ctcattccgt gaagtgataa gtgagagtgt ctgaattcct gcgcctttgc
     3301 tcacaatcca gacagtttcg cgacaattat cagaaataag tgacaaacgg cgtcgggtcc
     3361 gggacgttta tcgacgtaga tgctttcagc tgcggcgtgc cgaggtagag aaaaccgaca
     3421 attttatcct gctcacggca accgaatgct tcacgcacta ccggactttc agttaatgcg
     3481 ccactgcgcc agatgccgcc aaacccctgg gcaacggctg ccatttgcat cgccatgacc
     3541 gcgcatccgg cagacatttc ctgttcccat cgcgggactt tatgattttc ttcgcatttc
     3601 gccaccaccg tgatgatgag cggtgcgcgg aacggcgcat tacgggcttt gtcgatagct
     3661 ttgtcatcac taccggcagc aatcgccccc tgttccagta cggcgctgaa acgctcgcgc
     3721 ccttcccctt caatcacaaa aaaatgccac ggttgcatgg acttatggtc cggcgcgcgc
     3781 atacccgcac gcaggatgtt ttgcagttgt tcacccgttg gtgcgggttc agccaggcgg
     3841 gaggcgctac ggcgattgat caatagttcg agtgcatcca tttgattaac tcctgtcgtg
     3901 atatttattc acaaaattaa cacgagagtg gattttgtta cagcacagtc cgcaattcct
     3961 gctgacaagt gccggttggg tcattacgat aaccacatct attgcgcctg tgacaggtgt
     4021 gaccttaagt tgggagaata catgcgaacc ctttggcgat ttattgccgg attttttaaa
     4081 tggacgtggc gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt ctttattttt
     4141 ctcgtgctgg ttggtgtggg gatctggatg caggtcagtg gtggtgattc gaaagaaacg
     4201 gccagtcgtg gtgcactgct gctggacatt tctggtgtga tcgtcgataa acccgacagt
     4261 tctcagcggt ttagtaaatt aagccgccaa ctacttggtg ccagttccga tcgtctgcag
     4321 gaaaactcac tgtttgatat cgtcaatacc attcgccagg cgaaggatga ccgcaatatc
     4381 accggtatcg tgatggatct gaaaaacttc gcaggcggcg accaaccgtc tatgcagtac
     4441 atcggcaaag ccctgaaaga gtttcgtgac agcgggaaac cggtttatgc cgttggcgag
     4501 aactacagcc aggggcaata ttatctcgcc agtttcgcca ataaaatttg gctgtcaccg
     4561 caaggcgtgg tggatttgca cggttttgct accaacggtc tgtactacaa atcgttgctg
     4621 gataagctga aagtttccac ccatgtgttc cgcgtgggta cgtataaatc tgccgttgaa
     4681 ccgtttattc gtgatgacat gtcaccggca gcccgcgaag ctgacagccg ctggattggt
     4741 gagctgtggc aaaactatct gaatactgtt gccgctaacc ggcagatccc tgctcagcag
     4801 gtattccctg gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg cgataccgcg
     4861 aaatatgcac tggaaaacaa gctggtcgat gcactggcat cgagtgcgga aatcgaaaaa
     4921 gcactgacca aagagttcgg ctggagtaag gctgataaaa attatcgcgc catcagttat
     4981 tacgattacg cattgaaaac gccggcagat accggtgaca gcatcggtgt cgtcttcgct
     5041 aatggcgcaa ttatggatgg cgaggaaact caggggaatg ttggcggtga taccactgcg
     5101 gcacaaatcc gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct gcgtgttaat
     5161 agcccaggcg gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc agcagcccgg
     5221 gcagcgggta agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg tggttactgg
     5281 atttccacgc cagctaatta cattgtggct aaccccagca ccctgaccgg ttctattggt
     5341 atcttcggcg tgatcaccac cgtagaaaat agtctggatt cgattggtgt tcatactgat
     5401 ggtgtctcaa cttcaccgct ggcggatgtt tctatcacca gggcactgcc gccggaagcg
     5461 cagcagatga tgcagttaag cattgagaat ggctataaac gctttatcac gctggttgct
     5521 gatgcgcgtc attcgacgcc ggagcagatt gataaaatcg cccagggcca cgtctggacc
     5581 ggtcaggacg caaaagctaa cgggctggtc gatagtctcg gggatttcga tgatgcggtc
     5641 gccaaagcag cagagctggc aaaagcgaaa cagtggcatc tggaatacta cgttgatgaa
     5701 ccgaccttct tcgacaaagt gatggacaac atgtctggtt ctgtccgggc aatgttgcca
     5761 gatgcgttcc aggccatgtt acctgcaccg ctggcctcgg tagcctctac cgttaaaagt
     5821 gaaagcgaca agctggcggc gtttaacgac ccacaaaacc gttatgcgtt ttgcctgacc
     5881 tgcgccaacg tgcgttaaat cctgcaaaaa tgtggccgat atgctgtcgg ccacactcct
     5941 gaatcggact gtctttcact ccctctctcc actacattcc cttgctcatt tagcatctga
     6001 tatggagtca tcagtgaaca aggccccctc tttaattgcc gctatcgtcc tcggactggg
     6061 gattagcgcc tgtggttatt ttgttggcga tggtgttaaa catttaaaaa ccaacaaccg
     6121 ttatgtcaat gtgcgcgggc tttctgaaaa agaagttcgc gcagatacgg cggaattaac
     6181 aattgcgatt aactttaagg gtaatgttcc cggcgaactg ttcccgaaac tggaagaagc
     6241 gcagaaaaaa attgttgccg aactcaatgc tcaggggatt aacgagaaag agataattct
     6301 gggccaatgg acaagcaagc gaacagattc cttttatctg aaagatgatc ccacaatgcc
     6361 acgctataac gcagacgggt cagtgacgat aaaaacccat aatgttgctg ctgtcgaaaa
     6421 agtcgtcgcg aaattaaacg aactgcaagt cgctacggat ggcgctatcg ctgagagcaa
     6481 agtcgcctat cgctttaatg gtattggcgc actgcgcgct gagatgattg ctgccgccac
     6541 caaagatgca cgcaacgccg cactacaatt tgcaacggac tcaggtagcc aggtggggtc
     6601 aattagcgat gcctcacaag gcgtattcca gatctttgcc agtggcagtg atgaagatga
     6661 tcctacggcg attaataaaa cggtacgcgt agtcacgacc gtaacctatg cgttgcagga
     6721 ttaaccactt ttctccggcg ggacactaat ccctccgtcc tgccggactt ttctcatatc
     6781 cacagagtat ccaggtgcgc tcgctgcccc tcacgtatat acttttgctc tttcgatatc
     6841 attcatatca atatcatgca aaagaaatca atttacgttg cctacacggg cgggaccatc
     6901 gggatgcagc gttccgagca gggttatata ccggtgtcag gtcatctaca acgccaactg
     6961 gcgctgatgc cggaattcca tcgcccggag atgccagatt tcaccattca tgaatatacg
     7021 ccgctgatgg attcttcaga tatgacgccg gaagactggc agcatattgc tgaagatatt
     7081 aaagcgcact atgacgacta tgatggtttt gtcattctgc acggcaccga cacgatggcg
     7141 tataccgcct ctgcgctgtc gttcatgctc gaaaatctcg gtaaaccggt cattgtgaca
     7201 gggtcacaaa tcccgctggc tgagttacgc tctgacggac aaattaatct gctgaatgcg
     7261 ttgtacgttg cggcgaatta tccgatcaac gaagtaacac tcttttttaa taaccgactg
     7321 tatcgcggca accgcactac caaagcccat gccgatggtt ttgatgcgtt tgcctctcca
     7381 aaccttcctc cgttactgga agcaggtatc catattcgtc gtttgaatac gccacccgcc
     7441 ccgcacggtg aaggggaatt gatcgtacat ccaatcaccc cacaaccaat tggcgtagtg
     7501 acgatttatc cagggatttc tgctgacgtc gtgcgcaatt ttctgcgcca accggtgaaa
     7561 gcattgattc tgcgctccta tggcgtgggt aatgcgccac aaaacaaagc cttcctgcag
     7621 gaattacaag aagccagcga tcgcggtatt gtggtggtca acctgacaca atgtatgtcc
     7681 ggtaaagtga atatgggtgg ttatgccacc ggtaacgccc tcgcccatgc tggcgttatt
     7741 ggcggtgcag atatgactgt agaagccacg ctaaccaaac tgcattacct gcttagccag
     7801 gaactggata ctgaaaccat tcgcaaggcc atgagccaaa acctgcgcgg cgaactgacg
     7861 ccggatgatt aaggagactg caatgccccc tcgcgccctg ttactggtcg atttacaaaa
     7921 tgatttctgt gctggtggcg cgctcgccgt gccggaaggt gacagtacgg tggatgtcgc
     7981 taaccgcctg attgactggt gccagtcgcg cggtgaagcg gttatcgcca gtcaggactg
     8041 gcacccggcg aatcacggca gttttgccag ccagcacggt gtagagcctt atacgccagg
     8101 ccaactcgac ggtttgccac aaaccttctg gccagatcac tgtgtgcaga acagtgaagg
     8161 cgcacaatta catccgttac tgaaccaaaa agcgatcgca gctgtgttcc ataaaggcga
     8221 aaatccttta gttgacagtt acagtgccct ttttgataac ggccgtcggc agaaaacctc
     8281 tctcgatgac tggttacgcg atcatgaaat cgatgaattg atcgttatgg gcctggctac
     8341 tgactattgc gtgaagttta ccgtgctgga cgcgttacag ttaggttata aggtaaacgt
     8401 gattaccgat ggttgtcgtg gcgtgaatat ccagccccag gacagtgcgc acgcgtttat
     8461 ggagatgtca gcagctgggg caacgctata tacgctggca gactgggaag agacgcaggg
     8521 gtaattttac gctggcctac aattctgtac tggcattgta ggccaaataa aacacgtcag
     8581 tggcacatct ggcaattgat gccatcaacg aaagatttaa tttacctcag aaatctcttc
     8641 caacgacacc ttccgcgttt cgatgccaaa aatggagaga accagcgcac agagcaataa
     8701 catgacacca agtaccataa acaccgtgat cgacccataa tgtgttaata atgccgcaac
     8761 gccatagggc gtgaagactg cgacgatccg tccgacggca ttaacgaaac ccgaaccgcg
     8821 caggcgtaaa tgcgttggcc aaagctccgg gatataaacc gccgacgcga agcaaacata
     8881 catgtataaa aagaagatca tcaccaggcc atagattaaa atcgcccact ctgtagtctg
     8941 aattgaatag atatagccta acactgcaat aataataagt aaggtggagc caaataaccg
     9001 acgaggaaaa tgatcaataa ttaatgccgc aataaatatt cctaccggag cgccaatcat
     9061 aataacagcg gtcattaata ttgatttatc gacatcaatg ccggagttaa caaatatggt
     9121 cggtatccat acggtgatgg tataaagcga aatgttcatt gcaattaaaa cagtaatcgc
     9181 gactaatgta cgtcgtaaca tttcaccttt aaacaggagc cagaaagtac ccttgattac
     9241 tttaaccttg ctgttgctct gatacgaagt aagcgggggt aaacgaatac tcttctctct
     9301 ttcaatttgc tgctctactt cacgaagttg gcattctgca cctgcgattt gccctttccc
     9361 tgccagccat cgtggcgact caataaagta tttacctgag agaaaccagg ctaacagtat
     9421 gccaatacca cccaacagaa acattatccg ccaactaaaa aaagcgataa ccaccacacc
     9481 tatcgccgca gacagcatgg gcgaccagtt accaacaaat gagagccgcg cggaccattt
     9541 tccacgcacc gtcgcgggga taaactccgt aaatgaggca tagccaacca taatcagcgc
     9601 ccccattcct gttcccatca ggaagcgaaa gaagataagc cagtacatat cagggacaaa
     9661 agcggcccct gttgcagcaa taccgacgat gagaagattt atgcgaaacg ccctgcgccg
     9721 cccaaagtaa tcaccgatga acccaccggt aagagagccg atgaaataac caaacattaa
     9781 tgccgaggta aaggcggcat tgaggaaatt atttgaccat ccattgctta ccagctttgc
     9841 taagacgaca ttaccggagt aactcaaaaa ccc
//
LOCUS       DBFIFS010000165         9791 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.165, whole genome shotgun sequence.
ACCESSION   DBFIFS010000165 DBFIFS010000000
VERSION     DBFIFS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.165"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..184
                     /locus_tag="N7A07_003549"
     CDS             <1..184
                     /locus_tag="N7A07_003549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478143.1"
                     /translation="TCPGQNAKFFTGFWVKNFVGCSFTLDSQCGRLWLYQLPATGTHT
                     GVWHPAGNEQGPAATT"
     gene            156..665
                     /locus_tag="N7A07_003550"
     CDS             156..665
                     /locus_tag="N7A07_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478144.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(635..826)
                     /locus_tag="N7A07_003551"
     CDS             complement(635..826)
                     /locus_tag="N7A07_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478145.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(823..1245)
                     /locus_tag="N7A07_003552"
     CDS             complement(823..1245)
                     /locus_tag="N7A07_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2478146.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(1292..1717)
                     /locus_tag="N7A07_003553"
     CDS             complement(1292..1717)
                     /locus_tag="N7A07_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2478147.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(2135..2911)
                     /locus_tag="N7A07_003554"
     CDS             complement(2135..2911)
                     /locus_tag="N7A07_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478148.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(2957..3511)
                     /locus_tag="N7A07_003555"
     CDS             complement(2957..3511)
                     /locus_tag="N7A07_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2478149.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(3405..3626)
                     /locus_tag="N7A07_003556"
     CDS             complement(3405..3626)
                     /locus_tag="N7A07_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478150.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(3627..4310)
                     /locus_tag="N7A07_003557"
     CDS             complement(3627..4310)
                     /locus_tag="N7A07_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU2478151.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(4387..4692)
                     /locus_tag="N7A07_003558"
     CDS             complement(4387..4692)
                     /locus_tag="N7A07_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478152.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(4696..5598)
                     /locus_tag="N7A07_003559"
     CDS             complement(4696..5598)
                     /locus_tag="N7A07_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU2478153.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(6293..7264)
                     /locus_tag="N7A07_003560"
     CDS             complement(6293..7264)
                     /locus_tag="N7A07_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGU2478154.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(7264..8430)
                     /gene="sopA"
                     /locus_tag="N7A07_003561"
     CDS             complement(7264..8430)
                     /gene="sopA"
                     /locus_tag="N7A07_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGU2478155.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(9018..9773)
                     /gene="repE"
                     /locus_tag="N7A07_003562"
     CDS             complement(9018..9773)
                     /gene="repE"
                     /locus_tag="N7A07_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HGU2478156.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVVKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
BASE COUNT         2422 a         2749 c         2489 g         2131 t
ORIGIN      
        1 cacctgtcct gggcagaacg caaaattttt taccgggttc tgggtaaaaa attttgtggg
       61 ctgttcattt accctggaca gtcagtgcgg caggttgtgg ctgtatcagc tcccggcgac
      121 agggactcat acgggtgtgt ggcacccggc aggcaatgag cagggacccg ccgcaacgac
      181 gtgaagcgcg gtttgcgaaa cggcgttgcc ccttacatcc cggcggcctt ttgccgggat
      241 gccgggagcg caggccacgg cgcgacagag gctgcgcgac cggaacggtc gctgcaagcc
      301 agggtttcgc gtcagggatg gaagcccgcc agggcggaga cagcttcgct ggctccgttc
      361 acgacagccc gccccgccag ggggacgccc ggaaaagcca taagcaggag aggaacggaa
      421 agaagaggag gaagaggaag aggaaaaaga agaagaacag taacaggcac ggtaaccaca
      481 gaaaaataac atcagaaaaa aacattccgg acacatcaca aacaggaagg gtaacggacg
      541 acgcgtcagc gtggcccgct tcccttccgg caggcgtcag cctgccaggg gttttcgcgc
      601 gggctgacag gcggcctcca ccgccagccc tgtatcacct gtttctgctg ccctgtaaga
      661 tgtagtgacc gcccaggaaa acagcctgct gattcgccac aaaatcgcag atttcatcaa
      721 caatctgcgc ggtggtgagc ggtttctgcc ccatttcacg acgtcgcgca ttaatcacgg
      781 ctttgtaatt gcgcagaatt tcgcgggttt ctgtgctgat attcatgaca tgtgctcccc
      841 gtccagcgcc tgtaacacaa cgtccattgc ttcgcgttcc tccctgacga acgcatcacc
      901 gtcgcccccg ttctcactcc cgacggcata caggcgcttc atttcactgc ctgccagcgc
      961 catcactttt tccagcatca ccgccggaac tgtcacctga cggctggcac gcttttcttc
     1021 catcaactca cgaacaacgt cgtgaacaat gctgacatcc gcaccatatt caaaggcatc
     1081 gtccagccgc tgcatgacgg tttccagctc atcgtctgac aggttccagt cctgggctat
     1141 gtggcgcaca tccccacggg tcagcaccac ggcaaaaacg cattcactgt ccggcacgtc
     1201 tgtatccagt acatcgcgga tgatttcttt aacagtacag tacatgttct cgtctccttt
     1261 acaggggcgg cattgccgcc ccgtccgtta atcagtccag tgccgcgtaa atcgcgttgc
     1321 attcagggtg aaattcgatg tgacgccaca actgcgcatc gcgcatccgg taaagctggg
     1381 tcagtcctgc atcaccgctg tcgtggtaca gccacaactg gcggttaatc acaagggaag
     1441 tcagaatgat gccgcaggca tcagcactca cggtacggtc aaaccagttt gcaccgttca
     1501 ccatgtggaa acggtcgcca tccggcatca tgtaaccgcc accgtccggc aggcggatga
     1561 agtgccagaa gcctccctga tattccggca tcattttttg cgccagcgcg tacacatacg
     1621 cctcggcata cagaaaatcg ttgccaaaca ggtaaggcag aaaggagaga cggtcgcact
     1681 cttcaacatt cagagtgaca ggtttcgcat attgcatgat gttttcctcc gttgttcaca
     1741 tattccgcga caccgggcgg tgccgtgcct ccagaaaagc cgggcgataa ggcggggatg
     1801 cacagggcgc aagggaagcc gcagcctgac gcacacggct gacgggagtt gcagggtgag
     1861 gccgcgaaac ctgagcgggc gaacgaccgg aaaatcacgg cagacgggaa ggagggaccg
     1921 ggtgaccgca gcggcgaagc gtacccttgc atcaacgcca gcccggctaa gatggaggca
     1981 cggcaccgcc cgcacacgac gctgaggcca cataactgaa aaaccacaag gccatgccct
     2041 gcatggccgg gtaaagcgga gccagcggcg acttcctttg tgctctgtcc ggggaccggc
     2101 aggaaaaagg cggggattta ccccgccaac gtcatcattt ccggattttt gccagggcat
     2161 ccgccatgct gcggatttcg ttccgggctt cccggaccac ctcccgcaga acaccacggt
     2221 caaaccaccg gcggggcggt tcatccggta tacgcagcac atcgcaggcc agcccactgg
     2281 acaggatatg ttcaatacaa tcgctgacca gatacatttc caccgccgaa aaacgtgcgc
     2341 cccggttcag gatggcatcg agcaccacgg gcaggttttc gtcgtcctca tcgagccagc
     2401 aggggtgatc gctttccttc acgaccaggc gaaagcggcg cgtctggcgg gtcagtaacg
     2461 tctgaccggt ggtggtcagc gccgcccgga tacgccgcgt atcttctgcc acccggcaac
     2521 tgacactgcg taccgcatcg cgcaccgccg caagtacagc cctgatttcg tcttcggtca
     2581 gccattcacc ggcatacgga aaagacacct gcgttccgtc tgccggaagc accggcagcg
     2641 actgccactg actgccggac agaacaagtt ccgctccggt tccggtcagt ttctgaatgg
     2701 cggaggccag gtgcggcggc agtacgccgc cgtggcggtt aacgagtaac gtcgccagcc
     2761 ggggcgcatc cagcagaaca taaatgctga tccagcccgg atacttcggg ttacgctggt
     2821 cgacatccgt ctcttccggc cagccctgcg ccagcagcag gttacgggcg tgacgacaaa
     2881 tcagtgcgtt cagcgttgtg ttttcgttca tgattaatcg ttctccctgt atcgccgccc
     2941 gcaggcggcg ataacatcag ccgttttcgt gtgtgttgtt tttcagcagg atgcgggcct
     3001 gattaaccac cgcctgacgg cgggtaacgc attccgggac ggcgagaccg taatcacggg
     3061 cggcccattc acgtttccac agtgcctgtt cggcggtctg aatcagggac gccagcagtt
     3121 cagccgggac ggtcacctgg cggttttcgc tcacgttgct gataatctcc atcgccgcgg
     3181 cagaagaaat accggactca attcgctggt cttccgggat gtcctccagg cgcgccagga
     3241 cggtcctgat ttcaggatcg gacagggaaa tatccattcc gtcagcgagg gcctgaatct
     3301 cttccgggga ccagataacc agcatcagcg gtgtatatcc cggatacttt gctgccagct
     3361 cccggcatag cgtttcgcat gttccgtaca ttctcgtctc cttctcaatc tttaaagcac
     3421 gcatccagtt ccagaatttc ggtatacgcc tccatgaaca ggcgattggc gcgatacagc
     3481 gtctggcgtg ccaggcgttc aacaagcaca tcggaatcaa agcgacagcg atgctccata
     3541 tcgttcagcc tggcccgaag gtcacacatg gtgttggcca cctccgccac gtcggcgcga
     3601 agtttttcat gtccggcata gttcatttac gcagcctccg gcataaacca gtcatcattt
     3661 gcggccccct gctgcatggc ccgctgtacg gcagccagac gttgctgtcc ggcacggtga
     3721 tactgttcaa gcagctcgat accgatatac cggcgtccgg actgaagggc ggccacacag
     3781 gttgagccgc tgcctgcaaa cgggtccagc acaatcgcgt tcgggtgtgt gaagctctca
     3841 atcagtggtt gcaggctggt aacaggcttt tccgtcgggt gatgacgatt gcctgaatat
     3901 ttccagccca gcacgtcggg caatggcttc tgaggcagcg ccggacgccc ttttgccagg
     3961 atgtaagcgc attcgtggcg atagcccaca tatgcggcct tcgaagtgta ggttttggta
     4021 aaaaccaggt ggccaaccac gctgaatccc gcatttttcc aggcggccat aaagcgatcg
     4081 acgcggttcc agccatagaa gctcaccatc agcgcatctt ttttcagcac gcggtacatt
     4141 tcattacagg ccggttgcag ccattcatcg gttttatcgc cggcgatggt acgcccctga
     4201 cggtcacgga aaccgacgag atatggcggg tcggtgagga tgaaatcgac accattgccc
     4261 ggaatcgtgg ccataacacg cacgcagtca ccctggataa aacgggacat aacagcactc
     4321 cttctcttac agagggcacc gggagggcgc ccctgtggcg cccgttcccc gcggcctgtg
     4381 ggctgttcac cgcgtccggt aacgggtcag gccgaccgtg gaatatcgca gccgtcgtct
     4441 ttttgacggc gagaatatgc cgcgaaagcg gcttgaaggg cgttcatcag aaaagggcga
     4501 cgggctggcg tacactgtca ccgatgccag ccgggagcgg taaaagcgcc cgtcccgtgc
     4561 ccggccggac gcgggacact gaccacaggc agcgttcctg ccgcgccgcg tcagccggcg
     4621 cagtaaaaag aggagcccgg cgacatcctt taccggaaaa gaaacgggcg gttgcccgcc
     4681 cgtctccgtc atacttcatc caccgtaatg cgccaccttg tcgactatcc actccggcgc
     4741 ggtgacttcc ggcagctcat catcatctgt cggggaagac cattccagtt ccccccacag
     4801 cacatcaaca agctctccac gctcatatcg ctgccagcca cagaaattgc agccctgccc
     4861 ggcgtaccag tgctccagca tgcaactgaa acggcggctt aatactgcaa taacgtcgct
     4921 ttccggctga caccacggcg tgtcgaaatc aacctgaatg aagttgtctc cctgactgct
     4981 gatattcacc tcatacccac aaggccactt cacgccatac tgttccgtat accagtgata
     5041 tgcagaagga acaccgttca gaacaccacc gttaaagcca ttcacttcaa catccaggcg
     5101 agtggacaga accgtcagca ggtcaaacgg catggttttt tccggcagga cgttgtcaca
     5161 cagacggttc caccacacag atacatcctc gttactccag atgtcgcacc agtcacctct
     5221 ttttggggtg aaaagagcgg taatgctttc cctggcatca tccggtaatt cttcccagcg
     5281 acgccggcca gtaccgctct gcagccagag ttcatgcagc atcaggcagt tttgctcatc
     5341 cagaagtaca ccgtcctgca ggtgtgtcag ccagcgggtg aacgcgatat tctccggcga
     5401 tacaacgcca cgcccggcag ccgtcagtcc ggggcatggt tcaaaccgca catcttcagt
     5461 ggtctgcaga agtccggcac ttccggccag aaacagctga ataccttcat ttgtggcgcg
     5521 gcgataaagc ggtgtgactg caccgctggc cagtcgttta atctcagcga tctgtgccgg
     5581 ttcaccagaa aaatacatac gattcgagca ccagtttggc atgggaattc tcctctcaca
     5641 tgcggcctcc atcaggccgt gccgcacctg taaccgggcg accgcatacg aatgcaggtg
     5701 cggccccgaa gccgcagcgc gtggaggagg aacgacgaaa cgtgaccggg tgaggaggcc
     5761 ggcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca cggcctgggt
     5821 aaccaggtat tttgtccaca taaccgtgcg caaaatgttg tggataagca ggacacagca
     5881 gcaatccaca gcaggcatac aaccgcacac cgaggttact ccgttctaca ggttacgacg
     5941 acatgtcaat acttgccctt gacaggcatt gatagaatcg tagtctcacg ctgatagtct
     6001 gatcgacaat acaagtggga ccgtggtccc agaccgataa tcagaccgac aacacgagtg
     6061 ggaccgtggt cccagaccga taatcagacc gacaacacga gtgggaccgt ggtcccagac
     6121 taataatcag accgacgata cgagtgggac cgtggtccca gtctgattat cagaccgacg
     6181 atacaagtgg aacagtgggc ccagagagaa tattcaggcc agttatgctt tctggcctgt
     6241 aacaaaggac attaagtaaa gacagataaa cgtagactaa aacgtggtcg catcagggtg
     6301 ctggcttttc aagttcctta agaatggcct caattttctc tatacactca gttggaacac
     6361 gagacctgtc caggttaagc accattttat cgcccttata caatactgtc gctccaggag
     6421 caaactgatg tcgtgagctt aaactagttc ttgatgcaga tgacgtttta agcacagaag
     6481 ttaaaagagt gataacttct tcagcttcaa atatcacccc agcttttttc tgctcatgaa
     6541 ggttagatgc ctgctgctta agtaattcct ctttatctgt aaaggctttt tgaagtgcat
     6601 cacctgaccg ggcagatagt tcaccggggt gagaaaaaag agcaacaact gatttaggca
     6661 atttggctgt gttgatacag cgggtaataa tcttacgtga aatattttcc gcatcagcca
     6721 gcgcagaaat atttccagca aattcattct gcaatcggct tgcataacgc tgaccacgtt
     6781 cataagcact tgttgggcga taatcgttac ccaatctgga taatgcagcc atctgctcat
     6841 catccagctc gccaaccaga acacgataat cactttcggt aagtgcagca gctttacgac
     6901 ggcgactccc atcggcaatt tctatgacac cagatactct tcgaccgaac gccggtgtct
     6961 gttgaccagt cagtagaaaa gaagggatga gatcatccag tgcgtcctca gtaagcagct
     7021 cctggtcacg ttcattacct gaccataccc gagaggtctt ctcaacacta tcaccccgga
     7081 gcacttcaag agtaaacttc acatcccgac cacatacagg caaagtaatg gcattaccgc
     7141 gagccattac tcctacgcgc gcaattaacg aatccaccat cggggcagct ggtgtcgata
     7201 acgaagtatc ttcaaccggt tgagtattga gcgtatgttt tggaataaca ggcgcacgct
     7261 tcattatcta atctcccagc gtggtttaat cagacgatcg aaaatttcat tgcagacagg
     7321 ttcccaaata gaaagagcat ttctccaggc accagttgaa gagcgttgat caatggcctg
     7381 ttcaaaaaca gttctcatcc ggatctgacc tttaccaact tcatccgttt cacgtacaac
     7441 attttttaga accatgcttc cccaggcatc ccgaatttgc tcctccatcc acggggactg
     7501 agagccatta ctattgctgt atttggtaag caaaatacgt acatcaggct cgaacccttt
     7561 aagatcaacg ttcttgagca gatcacgaag catatcgaaa aactgcagtg cggaggtgta
     7621 gtcaaacaac tcagcaggag tgggaacaat cagcacatca gcagcacata cgacattaat
     7681 cgtgccgata cccaggttag gcgcgctgtc aataactatg acatcatagt catgagcaac
     7741 agtttcaatg gccagtcgga gcatcaggtg tggatcggtg ggcagtttac cttcatcaaa
     7801 tttgcccatt aactcagttt caatacggtg cagagccaga caggaaggaa taatgtcaag
     7861 ccccggccag caagtgggct ttattgcata agtgacatcg tccttttccc caagatagaa
     7921 aggcaggaga gtgtcttctg catgaatatg aagatctggt acccatccgt gatacattga
     7981 ggctgttccc tgggggtcgt taccttccac gagcaaaaca cgtagcccct tcagagccag
     8041 atcctgagca agatgaacag aaactgaggt tttgtaaacg ccacctttat gggcagcaac
     8101 cccgatcacc ggtggaaata cgtcttcagc acgtcgcaat cgcgtaccaa acacatcacg
     8161 catatgatta atttgttcaa ttgtataacc aacacgttgc tcaacccgtc ctcgaatttc
     8221 catatccggg tgcggtagtc gccctgcttt ctcggcatct ctgatagcct gagaagaaac
     8281 cccaactaaa tccgctgctt cacctattct ccagcgccgg gttattttcc tcgcttccgg
     8341 gctgtcatca ttaaactgtg caatggcgat agccttcgtc atttcatgac cagcgtttat
     8401 gcactggtta agtgtttcca tgagtttcat tctgaacatc ctttaatcat tgctttgcat
     8461 ttttttatta aatcttgcaa tttactgcaa agcaacaaca aaatcgcaaa gtcatcaaaa
     8521 aaccgcaaag ttgtttaaaa taagagcaac actacaaaag gagataagaa gagcacatac
     8581 ctcagtcact tattatcact agcgctcgcc gcagccgtgt aaccgagcat agcgagcgaa
     8641 ctggcgagga agcaaagaag aactgttctg tcagatagct cttacgctca gcgcaagaag
     8701 aaatatccac cgtgggaaaa actccaggta gaggtacaca cgcggatagc caattcagag
     8761 taataaactg tgataatcaa ccctcatcaa tgatgacgaa ctaacccccg atatcaggtc
     8821 acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa ctgccctcaa atttggcttc
     8881 cttaaaaatt acagttcaaa aagtatgaga aaatccatgc aggctgaagg aaacagcaaa
     8941 actgtgacaa attaccctca gtaggtcaga acaaatgtga cgaaccaccc tcaaatctgt
     9001 gacagataac cctcagacta tcctgtcgtc atggaagtga tatcgcggaa ggaaaatacg
     9061 atatgagtcg tctggcggcc tttctttttc tcaatgtatg agaggcgcat tggagttctg
     9121 ctgttgatct cattaacaca gacctgcagg aagcggcggc ggaagtcagg catacgctgg
     9181 taactttgag gcagctggta acgctctatg atccagtcaa ttttcagaga gacgatgcct
     9241 gagccatccg gcttacggta ctgacacagg gattcgtata aacgcatggc atacggattg
     9301 gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc ggttctgtaa cccgataaag
     9361 aagggaatga gatatgggtt gatatgtaca ctgtaaagcc ctctggatgg actgtgcgca
     9421 cgtttgataa accagggaaa agattcatag cctttttcat cgccggcatc ctcttcaggg
     9481 cgataaaaaa ccacttcctt ccccgcaaaa ctcttcaatg cctgccgtat atccttacta
     9541 gcttccgcag aggtcaatcc gaatatttca gcatatttaa caacatggat ctcgcagata
     9601 ccgtcatgtt cctgtagggt gccatcagat tttctgatct ggtcaacgaa cagatacagc
     9661 atacgttttt gatcccggga gagactatat gccgcctcag tgaggtcgtt tgactggacg
     9721 attcgcgggc tatttttacg tttcttgtga ttgataaccg ctgtttccgc catgacagat
     9781 ccatgtgaag t
//
LOCUS       DBFIFS010000166         9768 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.166, whole genome shotgun sequence.
ACCESSION   DBFIFS010000166 DBFIFS010000000
VERSION     DBFIFS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.166"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..550
                     /locus_tag="N7A07_003563"
     CDS             <1..550
                     /locus_tag="N7A07_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2478157.1"
                     /translation="NSGYVENWKEISKEIREKANYVCNDCGVNLSTAKNLCHVHHKNG
                     IKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLLKAESWNEIYDL
                     TDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIAVNLTPAEVEGW
                     RIYTVGELVKEIQTGAFTPAKL"
     gene            775..1017
                     /locus_tag="N7A07_003564"
                     /pseudo
     CDS             775..1017
                     /locus_tag="N7A07_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            1091..1942
                     /locus_tag="N7A07_003565"
     CDS             1091..1942
                     /locus_tag="N7A07_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGU2478158.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALVAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(2140..2327)
                     /locus_tag="N7A07_003566"
                     /pseudo
     CDS             complement(2140..2327)
                     /locus_tag="N7A07_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2447..3229)
                     /locus_tag="N7A07_003567"
                     /pseudo
     CDS             complement(2447..3229)
                     /locus_tag="N7A07_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3945..4403)
                     /locus_tag="N7A07_003568"
     CDS             complement(3945..4403)
                     /locus_tag="N7A07_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2478159.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            4526..5299
                     /locus_tag="N7A07_003569"
     CDS             4526..5299
                     /locus_tag="N7A07_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478160.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIVSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            5451..6767
                     /locus_tag="N7A07_003570"
     CDS             5451..6767
                     /locus_tag="N7A07_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478161.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(6966..7535)
                     /locus_tag="N7A07_003571"
     CDS             complement(6966..7535)
                     /locus_tag="N7A07_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2478162.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            7728..8195
                     /locus_tag="N7A07_003572"
     CDS             7728..8195
                     /locus_tag="N7A07_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HGU2478163.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            9199..9531
                     /locus_tag="N7A07_003573"
     CDS             9199..9531
                     /locus_tag="N7A07_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HGU2478164.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            9653..>9768
                     /locus_tag="N7A07_003574"
     CDS             9653..>9768
                     /locus_tag="N7A07_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HGU2478165.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVK"
BASE COUNT         2856 a         2217 c         2166 g         2529 t
ORIGIN      
        1 aaatagtggg tatgtcgaaa actggaagga aatatctaaa gaaatacgag aaaaggcaaa
       61 ttatgtttgt aatgattgtg gcgtgaattt atcaaccgcc aaaaacttgt gccatgtcca
      121 tcataaaaat ggcatcaaat atgataatca ccatgaaaac cttcttgttc tgtgcaagga
      181 ttgccatcga aaacagcccc tccacgaagg tatattcgtt acccaagcag agatggctat
      241 cattcaacgt ttacgttccc aacaagggtt attaaaagca gaatcctgga atgaaatata
      301 tgacctgact gatccatcag tgcatggtga tattaatatg atgcaacata aaggctttca
      361 acctcctgtt cctgggttag atcttccaaa ctcagaacat gaaattattg caaccgtaga
      421 agctgcatgg ccaggcctta aaattgcagt taaccttact cccgccgaag tcgaaggatg
      481 gagaatatat accgtgggtg agctggttaa agaaatacaa acaggagcct ttacgccagc
      541 aaaattgtaa attctaaaac tccgtgaaag ttaagacttt cacggaagat aaataaaatt
      601 tccctgattt gtggctcaaa ttacaaaagt agtttatggc ataacttgtc tgatttttat
      661 ggtgtaacag gtataaaagc atatgctatg gttcgcctca tacttaaaac ttccctcata
      721 tgggtgaagg ttaaagcttg gtagacagaa gacagtcaca atgaataaag caataaattg
      781 atgtcgcacg cgccgatggg catggcgggt aatgtgactt ttgtacacaa cggcaaggct
      841 tatgttactg gcggtgttaa ccagaacatc ttcaatggct attttgaaga tctcaacgag
      901 gctggaaaag attcagcagc tatagacaag atcaatgccc attatttcga caaaaaagca
      961 gaaaattatt tcttcaataa gtttttgttg tcttttgatc cctcaacaca gcaatggatc
     1021 ttcacttgca ggcttgaaca gcagaaaatt tgcgagcatg attgcaatta tttcactgcc
     1081 tgggatatca atggcttcca gtaaatcgct acagcaggca attgccaaca taaaaatctg
     1141 gcacaagggt gaacagcgcg cgccgcataa accattgtta ctgctatacg tattagcggg
     1201 atacctgaat ggacatccgc gccttttcga ttatggcacg gaaatctacg aacctctgca
     1261 cagtttactg gaacgttttg ggccacagcg ttcacagtac cgacctgata tgccgttctg
     1321 gcgattacaa ggcgacggaa tctggcaatt acacaatgcc gagcgttgct caacagcagg
     1381 gagcagccga caaccgccag tgaaggaact gaacgaatac cacgttgcag gcggtttcga
     1441 tgaacagcac tacgccctgg taaccggcaa taaaaaactc atcaatactc tggcgcaaca
     1501 gatactcgaa gcgcacttca ccgagagtat tcaggaagaa atcgctgacg agctgggttt
     1561 tgatctccag caaacccgta aacagcgcga cccgttattt cgcaaaaatg tattacgcgc
     1621 ttataactac cagtgcgcaa tatgtggctt taatatgcgc cacgatgaca ccaccgtcgc
     1681 gctggtagcg gcgcatataa aatggaaaca gcacggcggc ccttgtgaaa tccccaatgg
     1741 tctggcgctt tgcgcaatac accataaagc ctttgataaa ggatcgattg gcctggatga
     1801 aaatatgcgg gtactggtct cggatgcggt aaacggcgga gggatcgttg agagattatt
     1861 ctgggatttt gacgggaaaa ctattgcgtt gccgcaggtg cgtaaaaatt acccttatga
     1921 ggggtttgtg ggtgagagat agacattctg tttactaata atttatagcc attctctgct
     1981 cctgcatcag agatatgatg caggaatatt aaataccagc cacctataaa ttatgattta
     2041 ttaatttctg cttcagttaa atccctctga aaatagaccc gttttagttc catacatctt
     2101 taaaaatccc gaccaaataa tgagatcagc aaacatctat ttgaaacgtt ggttctcatc
     2161 ctcaagatcc ttgattattt taatatcaga aaattccata ccgccgtatc tggacttgca
     2221 gttgtagtaa gtggcttcag ataagccaca tctttaacag ttcgcccgta ttcaagcgat
     2281 ttaatcacgg cgataatctg atgctcagta aaaggggctt tacgaatagc gatctccttc
     2341 attggcagat tgattatgcc ggaggatctc taaatgtgaa tggcacgatt atgcgggata
     2401 tttactgtac cagtccaacc ctctgcttac tgcaatacca atcaagaggg atggtccaca
     2461 ttgacgtttc aaaatcgatt tctgaccagc gagcaaaatg cagcttactg gaccgaataa
     2521 agataagcag agtgagttct gtcgtacagt gggttagcgg tctgccggtg tagctgtcta
     2581 ttttgtcaag caactcaggg atacgcttta actccagcgc cggacgatgc tgtcgattac
     2641 tggaagcaac cgcccccgac atctcttgcg ccggatcgta atcaattaac ccgctttgca
     2701 ctgcataacg catgatggcc gtggtgcgct gttgaaggcg ggaagccact tcaagacgcc
     2761 cggatttctc cacggcttta ataggaatta acagatcacg ggtgcctagt tcagcaatgt
     2821 tgcgagtacc aattgtcggg aagatattgt cctccaggct cttttttaca cgattagcat
     2881 gatcttccga ccatttttgg ttggttacga gccactctct ggcaaccttc tcgaaagtaa
     2941 taatctcttt cgcctgctct tctttcacag cacgcttatg ctcacgagga tcgataccag
     3001 cagctaccag ctttctggcc tcttcccttt tcttccgcgc atccgccagt gaaacatccg
     3061 ggtaaacgcc gaacgccatc aaatgttgtt taccgccaaa acggaaacga aatcgccaat
     3121 actttgaacc gccaggtttt acaagtaagg acatgccgtc gccgtcaaca agtgaatact
     3181 ctttctcctc aggtttcgca gagcgaactt tagtatccgt tagggccata atggtcttcc
     3241 ttccattggt atctcgtgag tatacaagca atatcgaatc ggcaaatata ctcattagta
     3301 tactcacaag cctgttgatg tgggttggtt caggttgact tcagttgagg agaaaagctg
     3361 gaaaagcctt gcgtggcgcg gatttcaggc ataaaaaaag acctcagttg aggtctattt
     3421 acatactttt ggtgccgaag gccggactcg aacctaagtc tccataacct gctgtaatca
     3481 cgttcctatt agcctttaaa agaacaatcg taggaccttt ttgttctagg ggctacatct
     3541 taagtttgat ccgctggacg gaatcaaacc gagtgttcgt ggcggcacaa atcattaacg
     3601 tgccatacct gccttttgat gtttatggag gtctagtggg tcgggaggaa agaacatttg
     3661 tcagctatgt ggatgttaag taaacgccac tgcgttcgga ttttgaaacc agttaaaccc
     3721 cgtcagacct tgttcagaat aactatttaa ctcaatgtta tttatatgaa ttaagccatt
     3781 cagcgtcaaa gtaaaacgcc accgtaacgc ctacgtaacg cacccgcatt ttgctgtttt
     3841 attcactctt atagaaacaa agagctgatg aaacgtgagg ctatcagcat ctaatgaaca
     3901 ctgttcatcc atacagcatg aaaattcata aacttacact tgagctactt tctcagcttg
     3961 tcccaccgaa ccgcagatac aataccccag ttgccgctag gattaggcct acgccatttt
     4021 gctgtctcca tgctacgaag atactcaaga gcttcagaaa acgtgccaaa ccgcttctga
     4081 gccgttttat cacctacgcc aaacgagcca tcgcgagtgc ttgccagccc gggatgaaaa
     4141 cacgagccat cagctgcata aggaaccagt aattccgggt cactatcttc agatgaatca
     4201 gacaccaata gtcgccacgg ctcaacactc agagcattag ccagcacctc aatcgcatcc
     4261 aaagacgcat tagagccacc gcgttcaatg cggctcatat agctacgagc aaaaccacac
     4321 cgatcggcaa aggcttcttg gctcattccg gtggcaatgc gcaactcttt tactcgttgt
     4381 ccaaacagtt ttctcagggt cattttcatg acctgaattt gcgcagatgt ccactaaaga
     4441 gccacgctcc aatagtgaca ttttgaatca atggtctatg atgcgataac tgacaccgct
     4501 ggggtggatt ctgagggata acaacatgtc aaaaacacgg cttagtaagc ccggtttttc
     4561 agccaagtct gtgctgacca ttatgttaat cgctgcgtca tctgcatctt atgcagcaga
     4621 aagcatcgat caactggcga cgcgcatcgg tatggatcac ttccgggaag cgatcaaaaa
     4681 gggtgaacgc agcgccttca aaacgctgcc ggcaaaaggc tataaaaacg gtcttaccgg
     4741 gctgttaatc aaaactaaca cttacgttaa ccccgaatac agctttgctg attgcgatac
     4801 ggacaccgtt attatctcgg tgcaagaaca caaaacgctt cccggttgcc gtatatcttt
     4861 aaagctagcg acaaaagacg cagatggcag ttatcacgac aatggaattc gcgttgaata
     4921 cggtattgcc atcgtctcca aaaagtttat cgctaacaat tcagcgtggg cgcaacatgc
     4981 catcagcggt gataaagtgt tggaaggcgc tctccacccg gataaaacga ataacaataa
     5041 agatcaaatg aataacaata aagatcaaat gtgcatgggt atggctcaat ttaccgcaaa
     5101 cgtaaccgaa ggccgtaaca gtgggatgac gaaaagagaa tattatcagc gacttgataa
     5161 catgaacggt aaggccgacg tcacccagca gatgataacg ctctataaga cttttgttga
     5221 ctacgtatat gaacatccaa agcaagacgc aaagatttca gggacgtata tttatactaa
     5281 ctgtatgaac aacttctaga aacgtaggtg cctgaacatg atagaactca gctgccagca
     5341 tagagtggaa agcggaggct taccatcgta tattcatcgc gttatctttc cgtaactata
     5401 atctgtctca gttgatctag tgcctttcag ggctgaacta aggaataaag atgaatgacg
     5461 gtgttatgct agattctgac gttattgctg gtcttgagtg gttagcatca actcaagaga
     5521 acaaacaaca cttttaccag cgcttagcaa aagcacagca attttacata gctacaaccc
     5581 aaaaagctgc caatttcggg aaacagtttg acccagaatg gtatggttcc gacgtggttg
     5641 ctggttattt cgcacaagca aaatcgctta tcgataatag aaggtcttac gaagttacta
     5701 atgcttcaaa tataatacca tgggttaaac aattaggagt gtgtgctaaa tcgctcgata
     5761 gaattactgg cgctagagat cgcgcaatca gaatgcttaa gaatacaaca gtattaccag
     5821 acacagcctt attagagttg gttcttgctg gaaactatgc atcagaaggt tatgaagttg
     5881 agttcattcc agaacaaaaa gggatagcaa aaaccccgga gtttcgttgc cgccttggag
     5941 atggcgatta tttctttgtc gaatgcaaaa ggcttcagaa aggtccttac gtaaagcagg
     6001 aaatattaca gcatcatata cgctcacatc ttgccgaact tcatatcaaa tccaaaaaga
     6061 tgaacgtatg gactgatgtc acatatttat gtgaagttaa agacgcgcct gaaaactata
     6121 tcattagcca cttaaaaaac tttaaaggtg tattttatga atggaatgat gattatggaa
     6181 aaggaacgat tagaccggcc aacttaaatc ttatcaggga agatatcact gaaaacggct
     6241 cattactagt caacactaaa cttgctcgtc ttatcaaagg ctcccctctg gaagatgaaa
     6301 attaccaagt tgttgctatg ggacgaccag atgagcgcga ctcacgtttc ataaccaaag
     6361 ttaaacttgc ttctttgata acatggcgtt gtatcaatga aaaatctttc gatgcacgtt
     6421 cgcgacatgt aacaaaaaca ttagcagaga tagataacca gcttttggat tatggtctag
     6481 gcgtagggca tatagctttg gatgtagata ttcaaaagga tgttgccgat aaacgaaggg
     6541 aaaaaaacta tgctgccata gtaaattttg agcaaaaatc taaaatagtc cgcctgaatg
     6601 ttcattatct cgtgccacgc atagatgaga actctgcgtg gatggtagat gaaactgcgg
     6661 atgacttttt tacccatgat ctggtcaaat atgttatccc attgactaaa atattccccg
     6721 aggctgaggt atttgagaat gaccaacctg gctggcatca aaattaattt atttttgata
     6781 gtatgaacga gggtagcatt tctatacaaa tcagtgttcg gctgatagcg agttaaacag
     6841 tttactgaaa tgttctcagc aattagctgt tgccaggacc tcaaaaatcc gattctggcg
     6901 gaaagtgtgc tgttttacca agtattgctc gtatctgcaa agtgttagag catgttttaa
     6961 gtatatcagt aacccttatt cagtaatgca gcaagcgtcg ctcttgaaac accgtattca
     7021 cgcgccaatg cgctaacact aaccccagcg gcccttttgt ctaaaatcgc tccacgagct
     7081 tccggtgcga ttttagcagg cctgcccaat ttcctgcctt cagcttttgc ccgtaacagc
     7141 cccgcattag tccgttcaat cagcaggtca cgctccatct ccgcaaccgc tgctagcatc
     7201 gacaacagga gcttaccggc agcagaggtg agatcagtgg tgccaagctg atggacaata
     7261 accttgatgt ttcggtcagc caacgtcctg acagtttgca gaacgtcaat tgcatcacgc
     7321 ccgaggcggt ccagctttgc aactaccaac gtttccccat cgcggatttt gctgagcatt
     7381 tcaaaaaacg ctttgcgttg caccgcagga acctttccgc tgactacatc agaaaaccaa
     7441 aagtcgaacg tccacccagc ttgttcaagt tccatgcgct ggttctcagt atcctgctgg
     7501 gatgtcgaaa cacgcccgta gccaaaaata gccatagtca tctcctcatc cggcccctgc
     7561 cagaaaacgg tgatgaaaat aggatggcct gccggaaaag ccaaggctaa ttttccaaca
     7621 gtgtgaaagc gtgcggttgg aggatgccgg aaaacggtcg tttgttggcg cttaaaacgg
     7681 gtgaagacat gttaggctga aaccatcact tcataaggac aaccgaaatg attaaagacg
     7741 cgattttttc accgtgcggt aagtatcgct actcgttatc acgagtctgg gacgaatcaa
     7801 aaccgtatac ccttttcata gggctaaacc cttcatacgc cgatgccgag aaggatgatc
     7861 gcaccttgag ccgctgcata tcgtttgcca aaagctgggg ctacggtggc gtatacatgg
     7921 ccaacctgtt cgcattcgtt cacacccagc gacatgaaat gatgaaagcg tctgatccca
     7981 ttggcaaaga taacgatagc cacttgatac gtcttgttag cggtgccgga ctggttgtcg
     8041 ccgcatgggg aaatgaagga agacacctca aacgctcaac gaccgtccga cagctattgc
     8101 cagagagcac aatgtgcttc gtcttaaacg ctacaggtga gccgaagcac cctttgtata
     8161 tgaaaaacga cagtgttctc atacctttag gttaacatcg ataactgcgt tcagaatatg
     8221 aacgcagcca tatgatatat atgacttttc tttaaaatga tggtgtgcta gcttctctcc
     8281 tgacacctac ctcatgcggt aaaccggtcc gggtaggtgg agatcaccgc tgtttagcgg
     8341 aagacgtcag gatgggaact ctgcgtaacg ttgattgtgg taggtagggc gcgcaagcgt
     8401 ttgatgcagc caagcagagg gtgaaagtag ctatcggcgg attcttaagt agcttctttt
     8461 gcgcataatg catctttcaa gaaaggtcgt cacaccacga ggaagacttg atcttcatct
     8521 ggcaagggga accgagcaaa cctacaggct atcttctcag atggccttag ggttgatatg
     8581 tccggcttcc cccccgagga agggtttaca tcttaaagaa caagttatgt tttaacattt
     8641 ttaacaatta cacttctctg ctgtttggtg tcttatggcg ttcccgtcaa aagtaaagct
     8701 gccgttttta cctcacatgc gtaacaacca aacacgaacc agaagctgct aacgttcaac
     8761 gtgttcgcgg tgtaaatgca gccggataag ttgggagcga aaaagtggat gtcctaggtt
     8821 tggaagcgcc cctcttaagc attgcatccg ataatttccg tggcggtgag acagtcctta
     8881 tagaggcaat cagcctccaa agttaagcga cgcggtaaaa ctaaagctcg cccagatttc
     8941 tcattgattg ctcgaactcc gctacgcttg tttcaactgg cttcaccagt cactctatga
     9001 gagcgccgcg aattctgcac catgatttcg actaccttct ttaaaacact cgccagagag
     9061 ccttacagcg cgtttgaacc gcatatcgca gcttctgctt acggatgact gtctgattag
     9121 cagtagcttc atggcccagt ccggtttact ttattgctat caactaggag ccgcccaagg
     9181 gcaacacgga gataacgctt gagaaccgtc gaaatactgc aatacacctt acgcaaaggc
     9241 agcggtgccg cttttcacgc cataatgcag gaaatcagtg ttcccctaca ccaaagccac
     9301 gggatcgatg ttgtttcgtt tggcaactcg cttgatgacc tggattgcta ctacttgatt
     9361 cgcgcctttg acagcgctaa aagcatgact gcggttctcg atgcttttta cgcaagcgca
     9421 gactggcgca gcggcccacg cgaagacatc attggcagca tagagaactg cattaaaaca
     9481 gtgattagcc taccatcaga aagcgtgaaa gggctgcgga tgcaatcgta agtaatcaag
     9541 tgtattagct tagatttgac ctaaacagct ttgtgccaaa agtggactag gaaaagccta
     9601 gcgataaaga gttgagttta ataactacta actgaaattt aatgggtgat atatgaattt
     9661 agatgaagaa ttttacgaat tacaaaaact ctttgctcag aaggatctac tgactgagaa
     9721 aagacgctct agtggcggcg gattcatgga aattctcttg gtgaagag
//
LOCUS       DBFIFS010000167         9758 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.167, whole genome shotgun sequence.
ACCESSION   DBFIFS010000167 DBFIFS010000000
VERSION     DBFIFS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.167"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..119)
                     /locus_tag="N7A07_003575"
     CDS             complement(<1..119)
                     /locus_tag="N7A07_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478166.1"
                     /translation="MSYALIDNASLTAVERTLGDILVKNPDTINGDLVAFENL"
     gene            complement(306..482)
                     /locus_tag="N7A07_003576"
     CDS             complement(306..482)
                     /locus_tag="N7A07_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478167.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            785..1411
                     /locus_tag="N7A07_003577"
     CDS             785..1411
                     /locus_tag="N7A07_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2478168.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            1762..1929
                     /locus_tag="N7A07_003578"
     CDS             1762..1929
                     /locus_tag="N7A07_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU2478169.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(2009..3256)
                     /locus_tag="N7A07_003579"
     CDS             complement(2009..3256)
                     /locus_tag="N7A07_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU2478170.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3328..4242)
                     /locus_tag="N7A07_003580"
     CDS             complement(3328..4242)
                     /locus_tag="N7A07_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU2478171.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4458..5891
                     /gene="cscA"
                     /locus_tag="N7A07_003581"
     CDS             4458..5891
                     /gene="cscA"
                     /locus_tag="N7A07_003581"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU2478172.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5899..6894)
                     /locus_tag="N7A07_003582"
     CDS             complement(5899..6894)
                     /locus_tag="N7A07_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2478173.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            7137..7355
                     /locus_tag="N7A07_003583"
                     /pseudo
     CDS             7137..7355
                     /locus_tag="N7A07_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7373..8701
                     /gene="dsdA"
                     /locus_tag="N7A07_003584"
     CDS             7373..8701
                     /gene="dsdA"
                     /locus_tag="N7A07_003584"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU2478174.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8809..>9758)
                     /gene="emrY"
                     /locus_tag="N7A07_003585"
     CDS             complement(8809..>9758)
                     /gene="emrY"
                     /locus_tag="N7A07_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU2478175.1"
                     /translation="TETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIIIL
                     TVVSVIFLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLL
                     QETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRVLVTFSFLMYAVCYYWRSV
                     TFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFFRTLSGS
                     VGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEIT
                     QQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
BASE COUNT         2810 a         2352 c         2239 g         2357 t
ORIGIN      
        1 ataagatttt caaaagcgac taaatctccg ttaatagtat ctggattctt aacaagaata
       61 tcacccagtg ttctttcgac tgctgttaac gaagcattat caatcaacgc ataagacatt
      121 aaactacctc cattagataa tatctaataa cattatattt caattaggtt taaaaattgt
      181 gatacttatg acagatttga gtgacatttt atcaatctac tgattttgat ggtgaaattt
      241 attagaaggt atgcgtagtg ctacgggaga aatgagaaac gcgcccagca atggcgcatc
      301 ctttcttaag caagttggct agtatctata gcatccatcc aatcaggagc aattcccaaa
      361 cgttccgcga actcttcatt ggtcacgtct tccattgaga gactagccag acaatcgtta
      421 atttcagaat agacggggga aagtaatagg tattcccgca actctttcag gtctttttcc
      481 atcttaacac tccttgttaa tagaccgtcg aacaatggta cagatttgta ccattccaag
      541 tactggcaag caaccccacc agcacaacaa attatctcat acggtaaaaa cggtgtcggg
      601 ctgtttcttt cggctagctc aatatatcac cttaaacacc agccgttatc attgtctcaa
      661 ttgggtatac cgtaatgaca aggtgataca aagtctcaat agggttgtat aggaacattt
      721 tgactacaca aactgcatta tgtatcattt gcacttatat tcacaaccct tttgagacaa
      781 tgcaatgaac gttagaattt actgccgagc atcaactgag gggcaacacg ctgatcgagc
      841 acttaccagc ctacgtgaat tttcaaaatc aaaaggctgg cagattgcag gagagtatat
      901 agaaaatgct agcggggcga agcttgagcg cgtagagctt atgcggctat tgtctgaagc
      961 ccagtccggc gatctgttgc tagtggaagc tatagatcgt ctgagtcgcc tagaacatag
     1021 tgcatgggtc gaactgaaag atacgctcaa ccgaaaaggg ttaatcatcg tatcaatgga
     1081 tttaccaacg agctggcaga tggtcgagat ggcaggcaat gatctaacca gcggcatcct
     1141 gagagccgta aatgccatgt tgattgatat ccttgccaca atggcacggc aggattacga
     1201 aacacgccgt aaacgccaac agcaaggcat tgaacgagct aaaagcgaag gcatatacat
     1261 tgggagggcg aaaaatcagg aagcccgaga gatcgttaga gagatgttgg agcaaggagt
     1321 aaaacctgaa ctcatcatga aggcagcagg aatcagccga gcgacttact accggataaa
     1381 aaacgagcta ttgatcgtaa aatcagaata aaatttgaaa ggtcgtcagg cttgtttcat
     1441 caggtaaaaa ccagcatttc tgatcacaac gcttgggggc acctattggg gcactcacca
     1501 aaatcgaaag agaaataaac tacaattcaa attgttagct aatttttcga ttcctgcagg
     1561 gacaccagat acccttcaaa ccaaatctac cttcatcccg taaacgatgg gtttggcagc
     1621 acacttgccc tatatctact catttttact gcaacaggtt gaaatctcag cactgtcaga
     1681 aagcgctgat gactaaacag cccagggccg ggcgatgtaa ccatcacaca gaatcctgat
     1741 agcgaaatac ggcgtgactc gatacttcac tccgcaatgc attccttgat gaattcgcag
     1801 gcccgtgata cacgggacag gtcgcagaat gacgacaatg tcctggaaat cagcgaaccg
     1861 tgtatccgga gtacatttga gcgactgtac cagaacatga atgaggtgtt tggattaggc
     1921 gattattagc agggctaagc attttagtat tattattttc cggttgaagg atatggagat
     1981 atcgacaaca accggaaaaa gtttacgtct atattgctga aggtacaggc gtttccataa
     2041 ctatttgctc gcgtttttta ctcaggaaga aaatgccaaa tagcaacatc aggcagacaa
     2101 tacccgaaat tgcgaagaaa actgtctggt agcctgcgtg gtcaaagagt atcccagtcg
     2161 gcgttgaaag cagcacaatc ccaagcgaac tggcaatttg aaaaccaatc agaaagatcg
     2221 tcgacgacag gcgcttatca aagtttgcca cgctgtattt gaagacggat atgacacaaa
     2281 gtggaacctc aatggcatgt aacagcttca ctaatgaaat aatccagggg ttaacgaaca
     2341 gcgcgcagga aaggatacgc aacgccataa tcacaacacc gataagtaat gcattttttg
     2401 gccctacccg attcacaaag aacggaataa tcgccatgca cagcgcttcg agtaccacct
     2461 ggaatgagtt gagataacca tacaggcgcg ttcctacatc gtgtgattcg aataaacctg
     2521 cataaaagac aggaaaaagt tgttgatcaa aaatgttata gaaagaccac gtcccaacaa
     2581 taaatatgac gaaaacccag aagtttcgat ccttgaaaac tgcgataaaa tcctcttttt
     2641 ttacccctcc cgcatccgcc gctacgcact ggtgatcctt atctttaaaa cacatgttga
     2701 tcatcataaa tacagcgcca aatagcgaga ccaaccagaa gttgatatgg ggactgatac
     2761 taaaaaatat gccggcaaag aacgcgccaa tagcatagcc aaaagatccc caggcgcgcg
     2821 ctgttccata ttcgaaatga aaatttcgcg ccattttttc ggtgaagctg tcaagcaaac
     2881 cgcatcccgc cagatacccc aggccaaaaa agagcgcccc cagaattaga cctacagaaa
     2941 aattgctttg cagtaacggt tcataaacgt aaatcataaa cggtccggtc aagaccagga
     3001 tgaaactcat acaccagatg agcggtttct tcagaccgag tttatcctga acgatgccgt
     3061 agaacatcat aaatagaatg ctggtaaact ggttgaccga ataaagtgta cctaattccg
     3121 tccctgtcaa ccctagatgt cctttcagcc aaatagcgta taacgaccac cacagcgacc
     3181 aggaaataaa aaagagaaat gagtaactgg atgcaaaacg atagtacgca tttctgaatg
     3241 gaatattcag tgccataatt acctgcctgt cgttaaaaaa ttcacgtcct atttagagat
     3301 aagagcgact tcgccgttta cttctcacta ttccagttct tgtcgacatg gcagcgctgt
     3361 cattgcccct ttcgccgtta ctgcaagcgc tccgcaacgt tgagcgagat cgataattcg
     3421 tcgcatttct ctctcatctg tagataatcc cgtagaggac agacctgtga gtaacccggc
     3481 aacgaacgca tctcccgccc ccgtgctatc gacacaatcc acagacattc cagcaaaatg
     3541 gtgaacttgt cctcgataac agaccaccac cccttctgca cctttagtca ccaacagcat
     3601 ggcgatctca tactcttttg ccagggcgca tatatcccga tcgttctgtg tttttccact
     3661 gataagtcgc cattcttctt ccgagagctt gacgacatcc gccagttgta gcgcctgccg
     3721 caaacacaag cggagcaaat gctcgtcttg ccatagatct tcacgaatat tgggatcgaa
     3781 gctgacaaaa cctccggcat gccggatctc cgtcatcgca gtaaatgcgc tggtacgcga
     3841 aggctcggca gacaacgcaa ttgaacagag atgtaaccat tcgccatgtc gccagcaggg
     3901 caagtctgtc gtctctaaaa aaagatcggc actggggcgg accataaacg taaatgaacg
     3961 ttccccttga tcgttcagat cgacaagcac cgtggatgtc cggtgccatt catcttgctt
     4021 cagatacgtg atatcgactc cctcagttag cagcgttctt tgcattaatg caccaaaagg
     4081 atcatccccc acccgaccta taaacccact tgttccgcct aatctggcga ttcccaccgc
     4141 aacgttagct ggcgcgccgc caggacaagg cagtagccgc ccgtctgatt ctggcaagag
     4201 atctacgacc gcatccccta aaacccatac tttggctgac atttttttcc cttaaattca
     4261 tctgacttac gcatagtgat aaacctcttt ttcgcaaaat cgtcatggat ttactaaaac
     4321 atgcatattc gatcacaaaa tgtcatagtt aacgttaaca tttgtgatat tcatcgcatt
     4381 tatgaaagta agggacttta tttttataaa agttaacgtt aacaattcac caaatttgct
     4441 taaccaggat gataaaaatg acgcaatctc gattgcatgc ggcgcaaaac gcactagcaa
     4501 aacttcacga gcgccgaggt aacactttct atccccattt tcacctcgcg cctcctgccg
     4561 ggtggatgaa cgatccaaac ggcctgatct ggtttaacga tcgttatcac gcgttttatc
     4621 aacatcaccc aatgagtgaa cactgggggc caatgcactg gggacatgcc accagcgacg
     4681 atatgatcca ctggcagcat gagcctattg cgctagcgcc aggagacgag aatgacaaag
     4741 atgggtgttt ttcaggtagt gctgtcgatg acaatggtgt cctctcactt atctacaccg
     4801 gacacgtctg gctcgatggt gcaggtaatg acgatgcaat tcgcgaagta caatgtctgg
     4861 ctaccagtcg ggatggtatt catttcgaga aacagggtgt gatcctcact ccaccagaag
     4921 gaatcatgca cttccgcgat cctaaagtgt ggcgtgaagc cgacacatgg tggatggtag
     4981 tcggggcgaa agacccaggc aacactgggc agatcctgct ttatcgcggc agttcattac
     5041 gtgaatggac tttcgatcgc gtactggccc acgctgatgc gggtgaaagc tatatgtggg
     5101 aatgtccgga ctttttcagc cttggcgatc agcattatct gatgttttcc ccgcagggaa
     5161 tgaatgccga gggatacagt taccgaaatc gctttcaaag tggcgtaata cccggaatgt
     5221 ggtcgccagg acgacttttt gcacaatccg ggcattttac tgaacttgat aacgggcatg
     5281 acttttatgc accacaaagc tttgtagcga aggatggtcg gcgtattgtt atcggctgga
     5341 tggatatgtg ggaatcgcca atgccctcaa aacgtgaagg ctgggcaggc tgcatgacgc
     5401 tggcgcgcga gctatcagag agcaatggca aacttctaca acgcccggta cacgaagctg
     5461 agtcgttacg ccagcagcat caatctatct ctccccgcac aatcagcaat aaatatgttt
     5521 tgcaggaaaa cgcgcaagca gttgagattc agttgcagtg ggcgctgaag aacagtgatg
     5581 ccgaacatta cggattacag ctcggcactg gaatgcggct gtatattgat aaccaatctg
     5641 agcgacttgt tttgtggcgg tattacccac acgagaattt agacggctac cgtagtattc
     5701 ccctcccgca gcgtgacacg ctcgccctaa ggatatttat cgatacatca tccgtggaag
     5761 tatttattaa cgacggggaa gcggtgatga gtagtcgaat ctatccgcag ccagaagaac
     5821 gggaactgtc gctttatgcc tcccacggag tggctgtggt gcaacatgga gcactctggc
     5881 aactgggtta acataatatc aggtggaaca acggatcaac agcgggcaag ggatccgcgt
     5941 cacacttccc ccttcacgac cttcaataat atgcaatgca gcttcccgcc cgataatgtc
     6001 atgtggaagc tgaattgtgg tcagcggcgg taaaaacaga tgcccgacgc caaccagatt
     6061 atcaaagccc attacggcga catcctgcgg gatacgtacc cccttagcca ggagaacctg
     6121 ataagccaca aaggctgcgc gatcgttacc acatatcaga acatcaaaat ctggtttgcc
     6181 cgatttgaag tggtcattga gtaaacttgc gagatcggtg tagtgatcat cacctgttgc
     6241 catgtgaaat tgtttcacct cagccagatc tcgtccagca tcacgccagg cctgctcaaa
     6301 tccctgccga cgataccctg ttgccaacgc actttccggt agccagaagc ataacggttg
     6361 acgatagccc gccgcgagca aatgctgtgt tgattcatat tgtgcagtgt aatcatcagg
     6421 gatataactg ggtaacgctg ggtcatccgc cacacagttc gccaatacaa tattttcacc
     6481 atacagagac tcaggtagcg tgatatgtcg cagccccatt gtagtataga taatgccatc
     6541 cggacggtgg gcaagcagct gacgtgccgc gcgggcagcg tcatcttcag aaaaaatatt
     6601 gattaaaaaa ctattccagc cgaactcgct ggcggtttgc tcaatggcaa gcagaatatc
     6661 aacagagaaa ggagtggtag cagtgtcctg cgccagcacg gcgagagtcg acggcttacg
     6721 tccttgagcg cgcatcttac gggcggaaag atcaggaaca taattcaggg tctggattgc
     6781 ctgcaatacg cggtcacgcg ttgcaggacg cacagattct gcattatgca tcacccggga
     6841 gactgtcatc atcgacactc ccgccaggcg tgcgacatcc tttaatgaag ccatacccaa
     6901 gccgtttgcc gtaaaacggg cactgtagca gaaacagacg tcactggcga gatccaacga
     6961 aattctcgac gccctatcac ccgacacggc aatacaataa aaaataacaa taattgccgg
     7021 ataattgtac ccaattccgc ctctgttctc gccaacgagt gaccatatag gcaagtaaga
     7081 aaaacatgcc aactggcatc gccaacctgg agaagaaaat ataaatatgg atcattgtgc
     7141 tgccgctgta tcccgacatc agcccggaaa ttattgcgat tgctatcggt tcaggcgcga
     7201 ttggctgcac gatcgttacg gactcgcttt tctggctggt gaagcaatat tgcggcgcta
     7261 cgctcaatga aacatttaaa tactatacga cagcgacatt tatcgcttca gtcgtcgctc
     7321 tggcgggcac attcctgctg tcatttatca tctaagcgca aagagacgta ctatggaaaa
     7381 cgctaaaatg aactcgctca tcgcccagta tccgttggta aaggatctgg ttgctcttaa
     7441 agaaaccacc tggtttaatc ctggcacgac ctcattggct gaaggtttac cttatgttgg
     7501 cctgaccgaa caggatgttc aggacgccca tgcgcgctta tcccgttttg caccctatct
     7561 ggcaaaagca tttcctgaaa ctgctgccac tggggggatt attgaatcag aactggttgc
     7621 cattccggct atgcaaaaac ggctggaaaa ggaatatcaa caaccgatca gcgggcaact
     7681 gttactgaaa aaagatagcc atttgcccat ttccggctcc ataaaagcac gcggcgggat
     7741 ttatgaagtc ctggcacatg cagaaaaact ggctctggaa gcggggttgc tgacgcttga
     7801 agatgactac agcaaactgc tttctccgga gtttaaacag ttctttagcc agtacagcat
     7861 tgctgtgggc tcaaccggaa atctggggtt atcaatcggc attatgagcg cccgcattgg
     7921 ctttaaggtg acagtgcata tgtctgctga tgcccgggca tggaaaaaag cgaaactgcg
     7981 cagccatggc gttacggtcg tggaatatga gcaagattat ggtgttgccg tcgaggaagg
     8041 acgtaaagca gcgcagtctg acccgaactg tttctttatt gatgacgaga attcccgcac
     8101 gttgttcctt gggtattccg tcgcaggcca gcgtcttaaa gcgcaatttg ccgagcaagg
     8161 tcgtattgtc gatgctgata accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg
     8221 tcctggtggc gtcgcattcg gacttaagct ggcgtttggc gatcatgttc actgcttttt
     8281 tgccgaacca acgcactccc cttgtatgtt gttaggcgtc catacaggat tacacgatca
     8341 gatttctgtt caggatattg gtatcgacaa ccttaccgca gcggatggcc ttgcagttgg
     8401 tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg ctggatggct tctataccct
     8461 tagcgatcaa accatgtatg acatgcttgg ctggctggcg caggaagaag gtattcgtct
     8521 tgaaccttcg gcactggcgg gtatggccgg acctcagcgc gtgtgtgcat cagtaagtta
     8581 ccaacagatg cacggtttca gcgcagaaca actgcgtaat gccactcatc tggtgtgggc
     8641 gacgggaggt ggaatggtgc cggaagaaga gatggagcaa tatctggcaa aaggccgtta
     8701 ataacgtttc aacgcagcat cgcaatcctt tccctgggtg agcgatgctg ccgatggcgc
     8761 agacttaaga tccccagtct tacccgctat aaccccctgc ttttaccatc acccaacgcc
     8821 tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc gtaagtaaga taaaagcaat
     8881 agccgccata cggaaaatct catttgcaga aatagaaagt gactgctggg ttatttcatt
     8941 attaatttca ttaagaactc ctgaaagcga accatagtat ttatccataa tttgtgacga
     9001 tgaattaaac acggggttaa attgatcgat ggtttctgtc aactgactat ggtgtaacga
     9061 ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca actgatcctg acaaggtacg
     9121 aaaaaaatta ctcatactcg aggcattggc aaatttatta tctggcaagc ctgaaaacga
     9181 aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat ccctgaaaaa actgcggcaa
     9241 aatgatacct gtaaaatcaa tcgttggcat aaatgtcaca gaacgccagt aatagcaaac
     9301 cgcatacatc aaaaaactga atgtcaccaa cacccgcatg tctattttgt tgccataacg
     9361 tcctatcaaa ggtgaaatta atagtggcat gatgccgatg ggcgcataag caagtccggc
     9421 ccatatcgca ttatacccca tcgtttcctg gagtaactgc ggcataagga cgatcgctcc
     9481 agagtaaaat aaatacgcgc atgtgatact cacaatacca atggtgaagt tacgggactt
     9541 aaacaaactg agatcaagaa tcgggttctc tgaggtcgac tcccaaatga ctaaagagat
     9601 cagaaaaata actgatacta ctgttaatat tattattgta ctcgagttga accaatccag
     9661 atcgcgccct ttatcaagca taatttgcaa gccaccaaca ccgagcacta acagggtcag
     9721 tcctggtaga ttcattttga ccggtgaagt ctcagttt
//
LOCUS       DBFIFS010000168         9525 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.168, whole genome shotgun sequence.
ACCESSION   DBFIFS010000168 DBFIFS010000000
VERSION     DBFIFS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.168"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            214..1278
                     /gene="ulaG"
                     /locus_tag="N7A07_003586"
     CDS             214..1278
                     /gene="ulaG"
                     /locus_tag="N7A07_003586"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU2478176.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1386..2141
                     /gene="ulaR"
                     /locus_tag="N7A07_003587"
     CDS             1386..2141
                     /gene="ulaR"
                     /locus_tag="N7A07_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU2478177.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2138..2887)
                     /gene="yjfP"
                     /locus_tag="N7A07_003588"
     CDS             complement(2138..2887)
                     /gene="yjfP"
                     /locus_tag="N7A07_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2478178.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3069..3398
                     /gene="bsmA"
                     /locus_tag="N7A07_003589"
     CDS             3069..3398
                     /gene="bsmA"
                     /locus_tag="N7A07_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU2478179.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTLQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3547..3822
                     /gene="yjfN"
                     /locus_tag="N7A07_003590"
     CDS             3547..3822
                     /gene="yjfN"
                     /locus_tag="N7A07_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU2478180.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3939..5564)
                     /locus_tag="N7A07_003591"
     CDS             complement(3939..5564)
                     /locus_tag="N7A07_003591"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU2478181.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5648..6811)
                     /locus_tag="N7A07_003592"
     CDS             complement(5648..6811)
                     /locus_tag="N7A07_003592"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU2478182.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6814..7452)
                     /locus_tag="N7A07_003593"
     CDS             complement(6814..7452)
                     /locus_tag="N7A07_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU2478183.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7462..7860)
                     /locus_tag="N7A07_003594"
     CDS             complement(7462..7860)
                     /locus_tag="N7A07_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU2478184.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7878..8537)
                     /locus_tag="N7A07_003595"
     CDS             complement(7878..8537)
                     /locus_tag="N7A07_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU2478185.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8588..9286)
                     /locus_tag="N7A07_003596"
     CDS             complement(8588..9286)
                     /locus_tag="N7A07_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU2478186.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9305..>9525)
                     /locus_tag="N7A07_003597"
     CDS             complement(9305..>9525)
                     /locus_tag="N7A07_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU2478187.1"
                     /translation="ISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKS
                     SLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2484 a         2507 c         2262 g         2272 t
ORIGIN      
        1 gcgggattca cgcaacgaaa caattattca tttaaattca tttagttaat tgaattatac
       61 attccattaa atcttcccat actcaaaatc aacaacaact tccctggcaa aaagtcaaaa
      121 aatggtcatt gccttccaga aaaaatagtg tcaggatcaa tcaaaaccaa tcacatattg
      181 attcgaattg aactatcatg aaggtaaatg gcgatgagta aagtgaaaag tatcacccgt
      241 gaatcctgga tcctgagcac tttcccggag tggggtagct ggttgaatga agaaattgaa
      301 caagaacagg tcgctcctgg cacatttgcg atgtggtggc ttggctgcac cgggatctgg
      361 ttgaaatcgg aaggtggcac caacgtttgc gttgatttct ggtgcggcac tggcaaacaa
      421 agtcacggta acccgttaat gaaacagggt caccagatgc agcgcatggc tggcgtgaaa
      481 aaactgcagc caaacctgcg taccaccccg tttgttcttg atccgtttgc gattcgccag
      541 atcgacgcgg tactggcgac tcacgatcac aacgatcata tcgacgttaa cgtcgctgct
      601 gccgtgatgc agaactgtgc tgatgacgta ccgtttatcg gaccgaaaac ctgtgtggat
      661 ttgtggattg gctggggcgt accgaaagag cgttgcatcg tggtcaaacc gggcgatgta
      721 gtaaaagtga aagacattga aattcatgcg cttgatgctt tcgaccgtac tgcactgatc
      781 accctgcctg ccgatcaaaa agcggctggc gtactgccag atggcatgga cgatcgcgcg
      841 gtgaactacc tgttcaaaac gcctggcggc tccctgtatc acagcggcga ctcccactac
      901 tctaactatt atgcgaagca cggtaacgaa catcagatcg acgtggcgtt aggatcgtac
      961 ggcgaaaacc cgcgcggtat caccgacaag atgaccagcg ccgatatgct gcgtatgggt
     1021 gaagcgctga atgcgaaagt ggttatcccg ttccaccacg atatctggtc aaacttccag
     1081 gccgatccgc aagagatccg cgtgctgtgg gagatgaaaa aagatcgtct gaagtatggc
     1141 ttcaagccgt ttatctggca ggtgggcggc aaatttacct ggccgctgga taaagacaac
     1201 ttcgagtacc actatccgcg cggtttcgat gattgcttca ctattgaacc ggatctgccg
     1261 ttcaagtcat tcctgtaatc tgtcgtttat accggatagc gtggagaagt tcgcgctatc
     1321 cggcgtagta tttcctggct atttcaaata tcatctaaaa aaatcaaatt ttatcggaat
     1381 agctcatgac tgaagcacaa agacatcaaa tcctcctgga aatgctcgca caattgggct
     1441 ttgtgaccgt tgagaaagtc gttgagcgtc tgggaatttc gcctgccact gcgcgacgcg
     1501 atatcaataa acttgacgaa agcggcaaac tgaaaaaagt gcgcaatggt gcagaagcta
     1561 ttacccaaca gcgcccgcgc tggacgccga tgaatctgca tcaggcgcag aatcacgatg
     1621 aaaaagtacg tatcgctaaa gcggcctcgc agctggttaa tccgggcgaa agcgtagtca
     1681 tcaactgcgg ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag ccagtgcaaa
     1741 tcatcactaa ttatctaccg ctggcaaatt acctgatcga tcaagaacat gacagcgtga
     1801 tcattatggg cggacagtac aacaaaagtc agtccatcac tttaagcccg cagggcagcg
     1861 aaaacagtct ctatgccggg cactggatgt ttaccagcgg aaaagggctg accgcagaag
     1921 ggttgtataa aaccgatatg ctgacagcaa tggcagagca gaagatgctg agcgtggtag
     1981 ggaaactggt ggtactggtt gatagcagta agattggcga acgcgcggga atgcttttta
     2041 gccgtgccga tcaaatcgat atgcttatca ccggcaaaaa tgctaacccg gaaatcctgc
     2101 aacaactgga agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg cggaaaaatg
     2161 tcacggcagc atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc tgccatgaac
     2221 aggttagcag tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt agtgattcgt
     2281 cggcaggcac aacgtcatcg tcgaggccat accacagcag tagaggtctg tcactaagtt
     2341 gttccaggtg gtttgtcgct tcccactctg ccagtggcgc gacaatgtta ttgaattcat
     2401 tctgctgtgc taccgtttca ggtatcagcg gtggaaacag tgaacgggcg agtgatgtaa
     2461 aatagcccga tcccatcatg ctggcggtac atctcaccgt ggggtggcga gcggtaatcc
     2521 ccagtgccgt catcgcgccc atcgacgcac caccgactgc cagacggtca tcaagcagcc
     2581 acttttcttc ggctattgcc gcacgtaaag tagtgaattc ctgcatactt tgtagcaaga
     2641 tttgccagaa ttgatttaac cgccgcgctg cgtcaccact aaaacggcta ccgtgatcgg
     2701 gcgcatccgg catgatcacc cgcaaaccag cctgcgccag cgcaacggca aaatagctat
     2761 acaccagact ggacgaagta aagccgtgat aaaaaatcac gcacggtaac ggggtatctt
     2821 tttgtccgac aggataagca tgaagaacgg gaatatctgc cagctcgcgt gattctattt
     2881 caatcatctg tctctccttt ttgtctggta tgacatgaag aaatccttag cacaagtttc
     2941 tgctggcggt attagttcat aatgttgaga tgtgggttac gctttcgtca cgttttcatt
     3001 cgaaaatcgc gtaccaggta acaattcggg aacattcccc caaagttaaa ttaataaggc
     3061 actacactat ggttagcagg aaacgtaata gcgttattta ccggtttgcc agtttattat
     3121 tggtgttgat gttaagtgcc tgtagcgcac tgcaaggtac gctacagcca gcaccgccag
     3181 taacggacca tccgcaagag attcgccgcg accagacgca agggttacag cgaataggta
     3241 gcgtaagcac aatggttcgg ggttctccgg atgacgcatt agcagaaatc agagcaaaag
     3301 ctgtcgctgc aaaagctgat tattacgttg tcgtcatggt ggacgaaacc atcgtgacag
     3361 gacagtggta ttcacaagcc attttgtacc gtaaataaat cagacaactt acatagtcag
     3421 ctttacattg ctttgcgtcc ctatgcgttt acctgtgcac aataaattac cggctggaag
     3481 cctgggcgtt tattaccgcg atggaatgcc ctgcaacatg tggggaaacg aaaaatggag
     3541 ctgacgatga aacaattact tgcctcaccc tcgctgcaat tagtgactta tcctgcgagc
     3601 gccacggcac agtctgcaga attcgccagt gctgactgcg tgacgggcct gaatgaaata
     3661 ggtcagatct cagttagcaa tatttcaggg gatccgcagg acgtggaacg tatcgtggcg
     3721 ttaaaggccg atgaacaagg tgcgtcatgg taccgcatta ttaccatgta cgaagaccag
     3781 cagcctgata actggcgcgt acaggcgata ctgtatgcat gagtgtcatc tacagcaaat
     3841 gaacaatgcc ggatgcggcg cgagcgcctt gtccggccta caattttcac atatttcatt
     3901 tagttaatcg aaaccagcgt cgcatcagtc gtatacgctt acacacacac tccccccgtc
     3961 gcccgcagca ataaatcatt ctggatctgc tctgacaacc gtacgccgcc gcgcgtatct
     4021 aacatcacct gacaccacgc ctgcgccatt ggcggagaag catatttcaa catttgcgca
     4081 ccgcagccca gcaggaatag ctgatgagta atctctcgcc ccagttcttc agctggctta
     4141 cgcagctgct gctgtaaacg acgaaccgcg caatcaaaat agcgatcctg ccctttcact
     4201 tctacaaatg cttccgacaa taagtcgtat acgcccgctt gcttattgag aacgcgcagc
     4261 acatccaggc acataatatt gccggaacct tcccaaatac tgtttaccgg catctcccgg
     4321 taaagccgcg gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac ctccatcgct
     4381 tcggccacaa acggcatacc tcgtttgcag atcacaaatt ttgccgcagg cgtaaataaa
     4441 cgcgcccaca gagcttcttt ggcatcggcg cgccggtccc acgctcgcgc aagacgaaac
     4501 agcaacgccg tttgcccttc aagctgaagt gccatgcgac ttaagacatg acgcataagg
     4561 ggctgttgga tcaatggatt accaaaaaca tggcgttgat gcgcatgata aatcgccagc
     4621 gagaacgcac ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg cgtcatcccc
     4681 cccattttca ggatcagacg aattccttcc ccttcctgcc ccaataacca gccaatggca
     4741 tcctgaaact ccacttcaca actggcgtta gagcgattac ccagcttatc tttcagccgc
     4801 tcgaggcgaa tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa aaagcaggac
     4861 aaaccgcccg cggtctgcgc cagcaccaga tgcgcatcgc tttgcggaac tgagaaaaac
     4921 catttatgcc ccaccagccg ataagagcca tcctccagac gctctgcgcg ggtggtgttg
     4981 ctcatgacat cggaaccgcc ctgcttttcc gtcattccca tgccaatcaa caaaccgcgt
     5041 ttttgcccac ctggcaataa gtgagaatcg tagcgatcgc tcaacagcgg tgtggtccag
     5101 tcctgaaacg gcgcgggtaa catctgtaac aacaatggcg tggcggcaaa ggtcatggtt
     5161 atcggacata acgaccccgc ctcaacctgt gcgtgcaaca taaaacgcgc cgcgcgcgcc
     5221 acaaatgcgc cggagcgagc gtcttcttcc caggcaagat tgtgcacccg attggtacat
     5281 agtgcctgca tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag acggcgtcct
     5341 tgcgcatcgt agcgcaacag ttcaggcgga ttcacattcg ccagccgccc cagttcaagg
     5401 gattcagccg ttcctaactg ctgaccaata ctggctagaa aatcgctatc ccagccagca
     5461 ccttcacgcg ttaccgcttc gcaaagcgcg ccatcagaca ggtataagtt gctgttattt
     5521 aatggtatag gttgattaaa aacggtgtga gtttgccagt gcactgtgtc tccctccatc
     5581 aatggcagtc accattagta tggtcactgc catggattca tgacagaaag agattgctaa
     5641 aacattctta tccagcaatg taatgcggaa cgaaacgtga ggtgtctttg gtgatcagtg
     5701 tgttatcttc acggatcccc attccgcacg cttcatcatc gacaatccag ctaccgatga
     5761 gtgtgtagct atcgccaaac cgcggcagag gttgaaacgc ctggtagatc atcggttcat
     5821 cggcgtaatc accatcagcg tggtcaacga cgttattctg gccgtcgaaa atggtgacat
     5881 ttccgccttc gcgcgagtag atcggtttac gcacatagct ttcgccagcg gcaatctgcg
     5941 gtttttcacc ctcaaaccag gatgcaagaa ggttaggatg accagggaag aagcgccaaa
     6001 gcagcggcat tagcccttta ttactcaaga tacttttcca taacggctcc acccattgct
     6061 cgcgacgctt gcgcagcagc ggaccgttat cgtcacgcat catccactcc agcggataca
     6121 gcttaaatgc acgctggatg acattatcat caagatcggt cagtacgccg ccgacgccca
     6181 aaccgagatc ttcaatgtag ataaaccgcg actcctgccc tgcctgctgg gcgcagtcct
     6241 gcaagtacag cacggtactc ctgtcttcat cggtgtcctg acagcagcaa aaataaaacg
     6301 gttcccgact gtaaagctcg ctaaagcgtg aaatcaggcg ttcctgaata gcattgtact
     6361 gatcggcatc acgcggaata atgccgctgc gccgggcatc ctccagccac agccactgga
     6421 aataagccga ctcgtacaat gaagttggcg tatcggcgtt gtactccagc agcttcaccg
     6481 gcgcattgcc acaccaggca aaatccatcc gtccatacag cgaaggatcg cgggcgcgcc
     6541 agctttcagc gatcacatcc cagtacaacg gcgggatcgc cagttgcgtc aggatctctt
     6601 catcttttac cgcgcgatcc accacctcaa ggcacatctg atgcagttcc gcagtcggtt
     6661 tttcgatctg ctcttcaatc tggcgcagag taaaacggta agcccgactc tcatcccaat
     6721 agatttcatt gtcgatgata tgaaagtcga aaccgttgtc agcggcgatc tggtccagat
     6781 cccgtcgcac aggaacgttg tgtctcagca tgattagcct ccccaatgcc cacgggcgct
     6841 ggaagaacga ccatagccgc cgcgagaaac ggttgacgct tttttggtgg taaagccgcc
     6901 tgaagagtaa gactcttttt tgccagaacc ggagcgccag gagtaatcac cggaagtgtt
     6961 gcgccagacg gggcgggaag caaatgagga tccgccgctg ttatagacaa acggctcatc
     7021 gcgatcttta cgaataaccc ggcttaacaa aaatccagaa acgaccggga tccaactctg
     7081 ttcaacattg tcgtagtagc aattttcgta cttagactga cagttctgct gagtcatatt
     7141 cttcggaaca tcggcataaa atgccgcttt ggcgttgttc cagccacgcg cgcaaatgtc
     7201 ggcattatta ccgtcatcaa tacaatcctg cactgtcgaa taaaacgttc cgtcgccgtc
     7261 gttatcgaca tcgccactat cgccacaacc tttcaataca aaaaaagcgg caccgcccat
     7321 tagcgccaac gttaagtatt tttgtgcgta gcgattgcga cgcggttcaa aaggattatt
     7381 cggtctacca atgcgactga tcgctccgtg accaatttta ctgttatttc ttgatttgcg
     7441 ttttctggcc atgatcgtcc attaccatgt catacaagct gcgttaaaaa taccgccagc
     7501 cagcgcggcg gttcccataa acattcctgc tgcggtattg tgattaataa ttttttcgct
     7561 taatgcgggc atataaagtc tgacaccagc aaaaacgagt aactgaatca ccagtgcaat
     7621 cccgccccag gcgaaatagt ctggaatact caccgcattg attgccgcac tggataaggg
     7681 gatcacgtaa cccaacaatg taccgctgaa cgccagtgac gctgcggtat tattgttttt
     7741 gattaattgc cattcattgt gcggtgtaat tttggagtag ataaacagaa aaataatcac
     7801 catagccacg ccaataaaaa aataggcact aaaggcaaga agtgaatcca gtatatgcat
     7861 tgtaaacgtc cttgtaatta accaataata tgaagtgatg tcagcgggat atcgacaccc
     7921 aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt taaaagattc ctcaccattt
     7981 agcagcaaat attcgtaagt atcttcggtc acttgccgct ggtagcccat ggtaaaatta
     8041 tggacttccc atttagcatg attttgattt tctacttttt ccagcatgta gacgggttcg
     8101 atatttcccg gttcttcgct gttaaaaaat cgctgccagc gtttttcctg ccagtttaag
     8161 gtcattgccc ccatcgtttt ggcgttgatc gcctcgcgcc agtgattctc tttactgata
     8221 ccgtagctct cttcatagac aaagagcttg atatcatcaa tgtcatcaat atcttcgccg
     8281 ccggtggtat tgatttgcag aaactcatcg cctgaagtgt agtagcggaa aatctgacta
     8341 ccgccgccca gatcgatgtg gctgacagcg actaccgtaa attcctcacc cggcagcgca
     8401 atcagcaatt catcttccag caaacgaaac gctaacgtgt cgagcgtaaa gccactattg
     8461 agatgaagtc ccagcggacc gcgagcgata gcaggcttat tatccttgcc aaacagacgc
     8521 tggaaaaaac cagacatatt ttatccttaa aatagttgcc agccttgcag tgaggctggc
     8581 aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg gcgctacttt
     8641 tattgctacc gccaattccg gcttccgcca gcttttcgtc aaggtcgcga ccgtctgcga
     8701 ctttttctaa ctgtgcggca gcatccaggc gagcctgacg ttcggcctga cgcgtttgca
     8761 ggcgttttaa ggattctgct gctgtcgaaa cgctggaaga tgcgccaacg gtagaagtcg
     8821 ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc atttgctgct
     8881 caaactgagc gatacgctgc gctgtcgctg caactgcttt ttccacgcca tcacgggaga
     8941 cttccagatt cgacaacact tgctcttcag cggtaatgag attctcaagg cgcgcgattt
     9001 cttcagcaac ttcgttaatc aacgacggat taacgttctt gcttaacgct tccagcgtac
     9061 gggcttccag actggctttg cgctcacgta aatctttcag cttatcgtga cttaatttca
     9121 cccgcgccag cagatcaacg cgagattttc cggctttatc gagttcagct ttagcgtcac
     9181 gaatatgctg ttccagcatg cgcacaccct gggtttcttc aatggattct tccgcctggg
     9241 agataaacga tttccccagc gtaaataaac tttttaaaat tcccataaat acccgtcctt
     9301 gtaattagtg tgaatattct tctgtaattt cagccagatc caatgcgtta tctaccagcg
     9361 aggttatctc aagcaggata tcttcaagag aagatttaag cgataacgcc ccgaaaacaa
     9421 tgtaatactc ttcttgttgt acgctggaga ttcctaccga tgacaacggc atcatcttct
     9481 gatttcttaa taagaaggta ttaaattcat ctggattgct gatac
//
LOCUS       DBFIFS010000169         9524 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.169, whole genome shotgun sequence.
ACCESSION   DBFIFS010000169 DBFIFS010000000
VERSION     DBFIFS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.169"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..243)
                     /locus_tag="N7A07_003598"
     CDS             complement(<1..243)
                     /locus_tag="N7A07_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2478188.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGP"
     gene            complement(462..1439)
                     /gene="epmA"
                     /locus_tag="N7A07_003599"
     CDS             complement(462..1439)
                     /gene="epmA"
                     /locus_tag="N7A07_003599"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU2478189.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1764..3572
                     /gene="frdA"
                     /locus_tag="N7A07_003600"
     CDS             1764..3572
                     /gene="frdA"
                     /locus_tag="N7A07_003600"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU2478190.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3565..4299
                     /gene="frdB"
                     /locus_tag="N7A07_003601"
     CDS             3565..4299
                     /gene="frdB"
                     /locus_tag="N7A07_003601"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU2478191.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4310..4705
                     /gene="frdC"
                     /locus_tag="N7A07_003602"
     CDS             4310..4705
                     /gene="frdC"
                     /locus_tag="N7A07_003602"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU2478192.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4716..5075
                     /gene="frdD"
                     /locus_tag="N7A07_003603"
     CDS             4716..5075
                     /gene="frdD"
                     /locus_tag="N7A07_003603"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU2478193.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5138..6271
                     /locus_tag="N7A07_003604"
     CDS             5138..6271
                     /locus_tag="N7A07_003604"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU2478194.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6359..6892
                     /gene="blc"
                     /locus_tag="N7A07_003605"
     CDS             6359..6892
                     /gene="blc"
                     /locus_tag="N7A07_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU2478195.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6889..7206)
                     /gene="sugE"
                     /locus_tag="N7A07_003606"
     CDS             complement(6889..7206)
                     /gene="sugE"
                     /locus_tag="N7A07_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU2478196.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7382..7528)
                     /gene="ecnB"
                     /locus_tag="N7A07_003607"
     CDS             complement(7382..7528)
                     /gene="ecnB"
                     /locus_tag="N7A07_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU2478197.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7639..7764)
                     /gene="ecnA"
                     /locus_tag="N7A07_003608"
     CDS             complement(7639..7764)
                     /gene="ecnA"
                     /locus_tag="N7A07_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU2478198.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7816..8382)
                     /gene="efp"
                     /locus_tag="N7A07_003609"
     CDS             complement(7816..8382)
                     /gene="efp"
                     /locus_tag="N7A07_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU2478199.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8424..9452
                     /gene="epmB"
                     /locus_tag="N7A07_003610"
     CDS             8424..9452
                     /gene="epmB"
                     /locus_tag="N7A07_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU2478200.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2408 a         2453 c         2440 g         2223 t
ORIGIN      
        1 tggtccgaca ctattattca tccaggaata gataccgcct tcttctttgc gataagcagc
       61 tcccatttca gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata
      121 aaagggaatc gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa
      181 tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta tcgtgtgagg
      241 catgaaacca tccttcattt cagcgcgtgt ataaaacgcg cccactgaac tgcactgccc
      301 aacattgagc gggcagcgta aaaaccaata cagaattgat acctggatga tatcaaatat
      361 ccagtcttaa attgtggcct ttagcggaaa aatgaaaaca tatgctacag aatggcgctt
      421 atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg gtcaacgcta
      481 aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaaca
      541 cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctga
      601 tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt
      661 tgttgctgct cacgggcatc cgtcaattca tggaaaccat tcgccagctc aataccttta
      721 taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat
      781 gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttaccaat atttggttct
      841 acgccaaagg taaacagcaa ttgtagcaac gtgtcgcggt cttcttcggt atcagcaaca
      901 ttgctcaaat ccagtttcgc tgccacttcc cgcagttgcg ttttgtcggc agagagcggg
      961 tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccgggcag
     1021 tccagcacct gttgtaagag atcgtccacc tcgttcatca accggtacat atcatagtgc
     1081 ggtcgatacc actcaagcat agtgaactca gggttgtgat aacgccccat ctcttcatta
     1141 cggaaactgc ggcacagctg gaataccggc ccgcaaccgg caaccagcag gcgtttcata
     1201 tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggccaacg
     1261 aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc
     1321 gtctccacct ccagcactcc acgatcggca aagaaacgac ggatctccgc cataatcgcc
     1381 gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata
     1441 gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa
     1501 attttacgca ggaataaaac aacggtgggc agtgactaaa aaaagcacga tctgatggtt
     1561 tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg
     1621 atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata aaggagcagt
     1681 ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg ggctgtggga
     1741 taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg
     1801 ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa
     1861 tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct
     1921 ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg
     1981 gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg
     2041 aaatgaccca actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca
     2101 acgtacgtcg cttcggcggc atgaaaatcg agcgtacctg gttcgccgcc gataagaccg
     2161 gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt
     2221 ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag
     2281 caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta
     2341 ctggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg
     2401 gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc
     2461 atccaaccgg tctgccaggt tccggtatcc tgatgaccga aggttgccgc ggtgaaggcg
     2521 gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa
     2581 ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc
     2641 aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt ggtgatgtgg
     2701 tttatctcga cctgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct
     2761 gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc
     2821 cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta
     2881 aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg
     2941 gctccaactc cctggcggaa ctggtggtct tcggccgtct ggccggtgaa caagcgacag
     3001 agcgtgcagc aactgccggt aatggcaacg aagcggcaat tgaagcgcag gcagctggcg
     3061 ttgaacaacg tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc
     3121 gcgacgaaat gggcctggct atggaagaag gttgcggtat ctaccgtacg ccggaactga
     3181 tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca
     3241 ccgacacttc cagcgtgttc aacaccgacc tgctctacac cattgaactg ggccacggtc
     3301 tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac
     3361 accagcgtct ggacgaaggt tgcaccaagc gtgacgacgt caacttcctc aaacacaccc
     3421 tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta
     3481 cgctaccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag
     3541 cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg
     3601 cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac
     3661 gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg
     3721 agctaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac
     3781 gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa
     3841 gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa
     3901 agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact
     3961 aacatccaga ccccggcgca gatggcgaag tatcaccagt tctccggttg catcaactgt
     4021 ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct
     4081 gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt
     4141 atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctactgctcc
     4201 gaagtctgcc cgaaacacgt cgatccggct gcggccattc aacagggcaa agtagaaagt
     4261 tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa
     4321 acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg
     4381 cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat
     4441 tttcgggctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg tcgacttttt
     4501 acaaaacccg gttattgtga tcattaacct gatcactctg gcggcagctc tgctgcacac
     4561 caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat
     4621 gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat
     4681 cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt
     4741 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg
     4801 cgccggtgat gatcctgctg gtgggtattc tgctgccgct ggggctgttt ccgggtgatg
     4861 cactgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
     4921 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
     4981 atctgaaaat ccacgtacct gcgggcaaat gggttttcta cggtctggct gctatcctga
     5041 cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta aatccggccc
     5101 gcctatggcg ggccgttttg tatggaaacc agaccctatg ttcaaaacga cgctctgcgc
     5161 cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca acgatattgt
     5221 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
     5281 aatttatcag ggtaaacctt attactttac ctgggggtat gcggacatcg ccaaaaagca
     5341 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
     5401 gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata
     5461 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc tcgcaaccta
     5521 cactgctggc ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct
     5581 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
     5641 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
     5701 ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
     5761 gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc
     5821 gccaggggcg ttagatgctg aaacttatgg tgtgaagtcg accattgaag atatggcctg
     5881 ctgggtacga agcaatatga atccccgtga tatcaacgac aaaacacttc agcaagggat
     5941 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggtc tgggctggga
     6001 aatgctggac tggccggtaa atcctgacat catcgttaac ggcagtgaca ataaaattgc
     6061 actggcagca cgccccgtaa aagcgattac gcctccaact cctgcagtac gagcatcatg
     6121 ggtgcataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     6181 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     6241 cgcctggcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     6301 tttctgcgct tttccttgct gtcatctaca cttagaaaaa accagtaagg aaacatttat
     6361 gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg cctgcagttc
     6421 tcctacgccg ccgcgtggcg tgaccgtagt gaataatttc gacgctaaac gctatctcgg
     6481 tacctggtat gagattgccc gttttgatca ccgctttgaa cgtggactgg aaaaagtcac
     6541 cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag gctataaccc
     6601 tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg caccaactcg
     6661 cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg ttattgcact
     6721 cgatcgggaa taccgccatg cgctggtttg cggaccggac cgcgactacc tgtggatact
     6781 ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag tcgcgacccg
     6841 ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt agtgagtgct
     6901 gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccagac gcatcgggtt
     6961 agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc caatacccgt
     7021 ccacacggca taagccgtcc ctactggtaa cgatttcatc gcccaggcaa gtagcgccat
     7081 actgacaatc atcgccgtca cggtaataac actcggcgtc aaacgactaa agccgtgggt
     7141 atatttcagg cctacggccc ataccacttc cagcagacca gcaataacta agataatcca
     7201 ggacatatca ggctccggag aattggggcc gtccccggtg aaaagacgcg tttgcaggtc
     7261 gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcgggggaa tttcaactat
     7321 tacctttgtg actatcgcag aaaaaacaaa cggcacgaca cagctgtcgt accgttattg
     7381 cttattgctg cgctttcgtt gctgcaccag aaatcgcgtt accgccatca gaaatgtctt
     7441 caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa
     7501 agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt
     7561 ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat ctgatgattt
     7621 ttcggaagag aagaaaaatt agctggcagc gcgggagatg gagttgccga gatgtttgat
     7681 gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac
     7741 aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca gcctgcaggc
     7801 cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt
     7861 gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag tagacagggt
     7921 agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatcaacgat
     7981 ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac
     8041 gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt ttgcgtcagc
     8101 agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag
     8161 gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag atttgaaggt
     8221 tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc
     8281 tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat
     8341 tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg
     8401 ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag
     8461 aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt
     8521 tgaatataga cgcggacgaa aaactgttgg ccggacgcaa cgccaaaaag ctgtttgccc
     8581 tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac gatccgcttt
     8641 tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gcccggattc tccaccgacc
     8701 cgctggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac aaccgggcgc
     8761 ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc
     8821 cctatgccga aaatcagggc aacaagcgta actggcaaac tgcacttgag tatgttgctg
     8881 cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc
     8941 acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga
     9001 ttcacagtcg tctgccgatt gtgatcccgg cacgcatcac agatgcgctg gttgaacgct
     9061 tttcacattc tacgctgcaa atcttgctgg tgaatcacat caaccatgcc aatgagatag
     9121 atgaaacatt ccgtcaggcg atggctaagt tgcgccgggc gggcgttact ttgctgaacc
     9181 agagcgttct gttacgtggt gtgaacgata acgcacaaac gctggcaaac ctgagtaatg
     9241 cgttgttcga tgcaggcgtg atgccgtatt acctgcatgt gctcgataaa gtacagggcg
     9301 cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacac
     9361 tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa
     9421 cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt ttaatctttg
     9481 cccgttacat aacctttaat tattcatata cattgctgtt gtta
//
LOCUS       DBFIFS010000170         9408 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.170, whole genome shotgun sequence.
ACCESSION   DBFIFS010000170 DBFIFS010000000
VERSION     DBFIFS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.170"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            245..1660
                     /gene="tnaA"
                     /locus_tag="N7A07_003611"
     CDS             245..1660
                     /gene="tnaA"
                     /locus_tag="N7A07_003611"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU2478201.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1751..2998
                     /gene="tnaB"
                     /locus_tag="N7A07_003612"
     CDS             1751..2998
                     /gene="tnaB"
                     /locus_tag="N7A07_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU2478202.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3130..4305
                     /gene="mdtL"
                     /locus_tag="N7A07_003613"
     CDS             3130..4305
                     /gene="mdtL"
                     /locus_tag="N7A07_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU2478203.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4280..5239
                     /gene="yidZ"
                     /locus_tag="N7A07_003614"
     CDS             4280..5239
                     /gene="yidZ"
                     /locus_tag="N7A07_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU2478204.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5396..6145
                     /locus_tag="N7A07_003615"
     CDS             5396..6145
                     /locus_tag="N7A07_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU2478205.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6167..6733
                     /gene="yieF"
                     /locus_tag="N7A07_003616"
                     /gene_synonym="chrR"
     CDS             6167..6733
                     /gene="yieF"
                     /locus_tag="N7A07_003616"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU2478206.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6787..8124)
                     /gene="adeP"
                     /locus_tag="N7A07_003617"
     CDS             complement(6787..8124)
                     /gene="adeP"
                     /locus_tag="N7A07_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU2478207.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8290..8955
                     /gene="yieH"
                     /locus_tag="N7A07_003618"
     CDS             8290..8955
                     /gene="yieH"
                     /locus_tag="N7A07_003618"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU2478208.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9092..>9408)
                     /locus_tag="N7A07_003619"
     CDS             complement(9092..>9408)
                     /locus_tag="N7A07_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2478209.1"
                     /translation="KDYDDLLREIKELIISRNKSLEDKRTLFDNKRDNWNSIEVQDEV
                     NALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNILDWFERYPDIVQNIT
                     QA"
BASE COUNT         2315 a         2345 c         2318 g         2430 t
ORIGIN      
        1 aagattgtcg atcaccgccc ttgatttgcc cttctgtagc catcaccaga gccaaaccga
       61 ttagattcaa tgtgatctat ttgtttgcta tatcttaaat ttgccttttg caaaggccat
      121 ctctcgttta tttacttgtg ttagtaaatg atggtgcttg catatataca tggcgaatta
      181 atggatattg cagatgcaat attcacaggg atcactgtaa ttaaaataaa tgaaggatta
      241 tgtaatggaa aactttaaac atctccctga accgttccgc attcgtgtta ttgagccagt
      301 aaaacgtacc actcgcgctt atcgtgaaga ggcaattatt aaatccggta tgaacccgtt
      361 cctgctggat agcgaagatg tgtttatcga tttactgacc gacagcggca ccggggcggt
      421 gacgcagagt atgcaggccg cgatgatgcg cggcgacgaa gcttacagcg gcagccgcag
      481 ctactatgcg ttagccgagt cagtgaaaaa tatctttggt tatcaataca ccattccgac
      541 tcaccagggc cgtggtgcag agcaaatcta tattccagta ctgattaaaa agcgcgagca
      601 ggaaaaaggc ctggatcgca gcaaaatggt ggcgttctct aactatttct ttgataccac
      661 gcagggccat agccagatta acggctgtac cgtgcgtaac gtctatatca aagaagcctt
      721 cgatacgggc gtgcgttacg actttaaagg caactttgac cttgagggat tagaacgcgg
      781 tattgaagaa gttggtccga ataacgtgcc gtatatcgtt gcaaccatca ccagtaactc
      841 cgcaggcggt cagccggttt cactggcaaa cttaaaagcg atgtacagca tcgcgaagaa
      901 atatgatatt ccggtggtaa tggactccgc gcgctttgct gaaaacgcct atttcatcaa
      961 gcagcgtgaa gcagaataca aagactggac catcgagcag atcacccgcg aaacctacaa
     1021 atatgccgat atgctggcga tgtccgccaa gaaagatgcg atggtgccga tgggcggctt
     1081 gctgtgcatg aaagacgaca gtttctttga tgtgtacacc gagtgcagaa ccctttgcgt
     1141 agttcaggaa ggcttcccga cgtatggcgg cctggaaggc ggcgcgatgg agcgtctggc
     1201 cgtaggtctg tatgacggca tgaatctgga ctggctggct tatcgtattg cgcaggtgca
     1261 gtatctggtc gatggtctgg aagagattgg cgttgtctgc cagcaggcgg gcggtcacgc
     1321 ggcattcgtt gatgccggta aactgcttcc gcatatcccg gcagatcagt tcccggcaca
     1381 ggcgctggcc tgcgagctgt ataaagtcgc cggtatccgc gcggtagaaa ttggctcttt
     1441 cctgttaggc cgcgatccga aaaccggtaa acaactgcca tgcccggctg aactgctgcg
     1501 tttaaccatt ccgcgcgcaa catatactca aacacatatg gacttcatta ttgaagcctt
     1561 caaacatgtg aaagagaacg cgtcgaatat taaagggtta acctttacct acgaaccaaa
     1621 agtattgcgt cacttcaccg caaaactgaa agaagtttaa ttaatactac tgagtggcta
     1681 taaggatgtt agccactctc ttaccctaca tcctcaataa caaaaatagc cttcctctaa
     1741 aggtggcatc atgactgatc aagctgaaaa aaagcactct gcattttggg gtgttatggt
     1801 tatagcaggt acagtaatcg gtggaggtat gtttgcttta cctgtggatc ttgccggtgc
     1861 ctggtttttc tggggtgcct ttatccttat cattgcctgg ttttcaatgc ttcattccgg
     1921 gttattgtta ttagaagcaa atttaaatta tcccgtcggc tccagtttta acaccatcac
     1981 caaagattta atcggtaaca cctggaacat tatcagcggt attaccgttg ccttcgttct
     2041 ctatatcctc acttatgcct atatctctgc taatggtgcg atcattagtg aaacgatatc
     2101 aatgaatttg ggttatcacg ctaatccacg tattgtcggg atctgcacag ccattttcgt
     2161 tgccagcgta ttgtggataa gctcgttagc cgccagtcgt attacctcat tgttcctcgg
     2221 gctgaagatt atctcctttg tgatcgtgtt tggttctttc ttcttcctgg tcgattactc
     2281 cattctgcgc gatgccacca gctccactgc gggaacgtct tacttcccgt atatctttat
     2341 ggctttgccg gtgtgtctgg cgtcatttgg tttccacggc aatattccca gcctgattat
     2401 ttgctatggc aaacgcaaag ataagctaat caaaagcgtg gtcttcggtt cgctgctggc
     2461 gctggtgatt tatctcttct ggctctattg caccatgggg aatattccgc gagaaagctt
     2521 taaggcgatt atttcctcag gcggcaacgt tgattcactg gtgaaatcgt tcctcggcac
     2581 caaacagcac ggcattatcg agttttgcct gctggtgttc tccaacttag ctgtcgccag
     2641 ctcgtttttt ggtgtcacgc tgggattgtt cgattatctg gcggacctgt ttaagattga
     2701 taactcccac ggcgggcgtt tcaaaaccgt gctgttaacc ttcctgccac ccgcgttgtt
     2761 gtatctgatc ttcccgaacg gctttattta cgggatcggc ggtgccggac tgtgcgccac
     2821 tatttgggcg gtcattattc ccgcagtgct ggcaatcaaa gctcgcaaaa agtttcccaa
     2881 tcagatgttc acggtctggg gcggcaatct tattccggcg attgtcattc tctttggtat
     2941 aaccgtaatt ttgtgctggt tcggcaacgt ctttaacgtg ttacctaaat ttggctaaat
     3001 ccttcaagaa gccagtcatt cgctggcttc ttgcctctca ggaaatcact tatgtccaaa
     3061 tggcaactcg cctgatcctc cttcaccacg tatgctttgc gtcaccttac tatcaggacg
     3121 ctttagccca tgtcccgctt tttgatttgt agttttgccc tggttttact ttatcccgct
     3181 gggattgata tgtacctcgt tggtttaccg cgcatcgccg ccgatctcaa tgccagcgaa
     3241 gcgcagttac atattgcgtt ctccgtttat ctggcaggta tggcagctgc gatgttattt
     3301 gccggtaaag tggccgatcg ttcagggaga aaaccggtcg ccatccccgg cgcggcgcta
     3361 tttattatca cttcggtgtt ctgttcactg gctgaaacca gcacgttatt tcttgcaggg
     3421 cgctttctac aggggttggg cgcgggctgt tgttacgtgg tggcattcgc cattttgcgc
     3481 gacacgctgg atgatcgacg tcgggcaaaa gtgctgtcgt tactcaacgg cattacctgc
     3541 atcattccgg tgttagcgcc ggtgctcggt catctgatta tgcttaaatt cccgtggcag
     3601 agtctgttct ggacgatggc aataatgggc atcgcggtac tgatgttgtc tttgtttatt
     3661 ttaaaagaaa cgcgccctgc cgcccccgca gcttcggaca aatcacgaga aaatagcgag
     3721 tcgctgctta atcggttttt cctcagccgt gttgttatca ccaccctcag cgtttcggtg
     3781 atcctcacct tcgtcaatac atcgccggta ttgctgatgg aaatcatggg ttttgagcgc
     3841 ggagaatacg ccaccattat ggcgttgacc gctggcgtca gcatgaccgt ttcattttcc
     3901 acgccatttg cgctgggaat ttttaagcca cgtacgttga tgatcacctc acaggtgtta
     3961 ttcctggcgg cgggcatcac tcttaccgtt tcaccttccc atgcggtttc tctgtttggt
     4021 atcacgctga tttgcgccgg tttctcggta ggttttggtg tggcgatgag tcaggcgtta
     4081 gggccgtttt cattacgcgc gggcgtagcc agctcgacct taggtattgc gcaggtttgc
     4141 ggttcgtcac tgtggatttg gctggcagcg gtggttggta tcagcgcatg gaatatgctg
     4201 atcgggattc tgattgcctg tagcatagtg agcctgttgc tgattatgtt cgtcgcgcct
     4261 ggacgcccgg ttaccgctca tgaagaaatc catcaccacg cttgatctca atctgctgct
     4321 ctgtctgcag ctgctgatgc aggagcgcag cgtaaccaaa gcggcgaagc ggatgaacgt
     4381 gacaccttcg gcggtgagta agtcgctggc aaagttaaga gcgtggtttg acgacccgct
     4441 ctttgtgaac tcaccgttgg ggctgtcgcc cacaccgcta atggtcagca tggagcaaaa
     4501 tctggcggag tggatgcaaa tgagcaacct gctgctggat aaaccgcacc accagacacc
     4561 gcgcggcctg aagtttgagc tggcggcaga atcaccgcta atgatgatca tgcttaatgc
     4621 gctgtcgaaa cagatctacc aacgttaccc gcaggcgacc atcaaattac gtaactggga
     4681 ttacgattcc ttagatgcca ttactcgtgg tgaagtggat atcggttttt ccggtcgcga
     4741 aagtcatccc cgttcgcggg agctgttaag ctcgctaccg ttagccattg attatgaagt
     4801 gttgtttagt gatgtgccct gcgtctggtt acgcaaagat catccggcac tgcatgaaaa
     4861 gtgggatctc gatacctttt tacgttatcc gcatatcagc atttgctggg aacagagcga
     4921 tacctgggcg ctggacaatg tgttacagga gctggggcgc gaacgtacga ttgcaatgag
     4981 cctgccggaa ttcgagcagt cactgtttat ggcagcgcag cccgacaatc tgctcctggc
     5041 gaccgcgccg cgctactgtc agtactacaa tcaactccac caactgccgt tggttgctct
     5101 tcctctcccg tttgacgaaa gccagcaaaa aaagctggaa gtccctttta ccctgctgtg
     5161 gcataaacgg aacagccata atccgaagat cgtctggtta cgggatacca ttaaaaatct
     5221 ttacgcgtcg atggcataac cgaatcgtat gaaacgcgac ccgatttcac aataaaatgt
     5281 aaaaaagttg taataagctt gtctgaatcg aacttttagc cgctttagtc tgtccatcat
     5341 tccagtaaat gattactctt gtattcataa tggaccatta agcatggagc gaaaaatggc
     5401 gactcacttt gcccgaggga ttttaacgga aggacatctg atttctgttc gtctcccctc
     5461 ccagtgtcat caagaagccc gaaacattcc ccctcatcgt caaagccgtt ttctggcgtc
     5521 cagaggttta ctcgcagaac tgatgttcat gctgtatggc attggcgaat tgccggaaat
     5581 cgtcaccctg ccgaaaggta aaccggtttt cagtgataaa aatttgcctt cgttttctat
     5641 ttcctatgcc gggaatatgg ttggcgtggc gttaacaacc gaaggtgaat gtggcctcga
     5701 tatggaactg cagcgtgcga cgcgcgggtt tcatagccca cacgcgcccg ataaccacac
     5761 attttccagc aatgaatcgc tatgggtcag taaacaaaat gatccaaacg aagcgcgggc
     5821 gcagctcatc acgctgcgcc gaagcgtgct aaaactaacc ggtgatgttt tgaatgacga
     5881 tccgcgcgat ctgcaactgc tgcccattgc cggacgcctg aaatgtgctc atgtaaatca
     5941 tgtagaagcg ttatgcgacg cggaggacgt gctggtgtgg tccgtggcgg tcacgcccac
     6001 gattgaaaag ctcagtgtct gggagttaga tggcaaacac ggctggaaaa gcctgccgga
     6061 tattcacagc cgcgccaaca atcctaccag ccggatgatg cgttttgccc agctctctac
     6121 catgaaggct ttttcgccaa attgatagac aaccacagga gtcattatgt ctgaaaaatt
     6181 gcaggtggtt acgttactgg ggagcctgcg caaaggctca tttaatggca tggttgcacg
     6241 taccctaccg aaaattgctc cggcgagcat ggaagtcaat gcgttacctt ccattgccga
     6301 cattcccctg tatgacgctg acgtacagca ggaagaaggt tttccagcaa cggttgaagc
     6361 tctggcggaa cagatccgtc aggctgacgg tgtggtgatc gtcacgccgg aatataacta
     6421 ttcggtaccg ggtgggctga aaaatgccat cgactggctt tcccgcctgc cggatcaacc
     6481 gctggccggt aaaccggtgt tgattcagac cagctcaatg ggcgtgattg gcggcgcgcg
     6541 ctgtcagtat cacctgcgcc agattctggt tttcctcgat gcaatggtga tgaacaagcc
     6601 ggaatttatg ggcggcgtga ttcagaacaa agttgatccg caaaccggag aagtgattga
     6661 tcagggtacg ctggaccacc tgaccgggca attgaccgca tttggcgagt ttattcagcg
     6721 agttaagatc taaataaaaa acccgccagc aatcatgcat ggcgggtttt taacgcgcta
     6781 tcgattttag tgagcgtcga taaatacaat cttcaggata aacagcagcg caacgatgat
     6841 tacgcacggg ctaaggtcac gaatacgccc ggtaccaatc ttcatcacgc agtaggagat
     6901 aaagcccagc gcaatacctt cggtaatcga gaagctgaac ggcatcatca cagcggtaat
     6961 aaacgccgga acagattcag taagatcctg ccagttcacg cgagcaagac ttgaggtcat
     7021 caacacgcca acgtaaatca gcgcgccagc tgcagcgtag cctggcacca ttcccgccag
     7081 cggcgacaga aagataacca gcaggaacag cagaccaaca accactgccg tcagaccggt
     7141 acgaccgcca accgatacgc cggaagagga ctcaatataa gccgtaacgg aagaagtacc
     7201 gataaacgaa ccggtcacgg aagagatact gtcgacatac agcgcctgct tcatgcgcgg
     7261 gaatttcccc ttctcatctg ccagaccggc tttatcggtc acgccaatca acgtgccgga
     7321 ggagtcaaac aggttgacca gcatgaaaga gaaaatcacc cctgccagcc cgaggttaaa
     7381 cgacccggct aaatctacat gacccacgac agtcattacg ctcggcggcg cagaaacgat
     7441 gccattgtag tggacatcac ccagcatcca gcccagcagc gttgtcacca cgatagaaac
     7501 cagcaccgct gcgtgaatgt tgcgtgaggc cagaatagca atgatgaaga agccgaggat
     7561 ccccagcagc acgctgtgag aagtcagatt accgatgctg accagcgttt ccgggttagc
     7621 gacaatcaca cctgcgtttt tcagccccat catgccaatg aacagaccga taccgctggt
     7681 aatgcccaca cgcagactca ccggaatgtt ggcaatcatc cagtagcgaa cgcggaaaat
     7741 cgtcagtaac agcagaccaa tcgcgcccca gaagattgcg cccatcccga cttgccacgg
     7801 caagcccatc gcctgcacaa cgacaaaagc gaagaacgca ttcaggccca tagcgggtgc
     7861 cagtgcaact ggcaggttag caaacagccc catcataata ctgccgaatg cagcaatcag
     7921 acaggtagtg acgaagacgg cgctggtatc catgccagca acgccaagaa tttgcgggtt
     7981 aacaaaaacg atgtaaacca tcgtcaggaa ggtggtaaaa ccggcgatca cttcggtccg
     8041 tgccgtcgtg ccatgttcgc gcagtttaaa cacgcgttcc agcatcccct ggccagaagc
     8101 ctgggttgtg tgttgttgac tcattatcta tttccgaaca aggagggaaa atccgtcgct
     8161 atcgtatacc aaaatgagac aatatggacg gctgaagata acttttttca ttgtatttgc
     8221 ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa ctaacgataa
     8281 aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac gctggtcgat
     8341 agcgaggtca tttgctctcg cgcctatgtc acgatgttca gggaatttgg cattcacgtt
     8401 gacctgaaag agatttttac ccgttttaaa ggcgtaaaac tctacgaaat catcgatatc
     8461 atcagtaaag aacagggcgt gacgatggtc caggccgatg ccgagcacgt ttaccgcgca
     8521 gaagtagcac gtctgtttga taccgaactt gaggacatcg caggagcgaa cgaattgctg
     8581 gcgagcatta acgtgccgat gtgcgtcgtc tctaacggtc cggtcagcaa aatgcagcat
     8641 tcgcttggta agctcaacat gctgcactac ttcccggaaa aactgttcag cggatatgac
     8701 attcagcgtt ggaagcccga tccggcatta atgttccatg cggcaaaagc gatgaatgtg
     8761 aacgttgaaa actgcattct ggtcgatgac tccagcgctg gcgctcaatc agggatagat
     8821 gccggaatgg aagtgtttta cttctgcgct gacccgcaca ataagccgat cgttcacccg
     8881 aaagtcacca cctttaccga gctgtcgcag ctgcctgcgc tctggaaggc acgcggctgg
     8941 aatataacgc gataatcgcc tccttacttc aacacttaca catcagtcac gctggtttga
     9001 gatacagaac accaggtgtc tgatgagtaa gcgtctattc cctatgaata atttatgtcg
     9061 ttattcgtcc tgtcatccac cctctgtatg gttaagcctg ggttatattt tgtacaatat
     9121 cgggataccg ttcgaaccaa tccagaatat tacgcatcgc gtcactgatg tttttatcgt
     9181 ctttatattg attctcaagt ctattatttc gaacaatagt aagcttagaa cgtattttat
     9241 catcacaatc gacaattttc gcattcaatg catttacttc atcctgcact tctatactat
     9301 tccaattgtc ccttttgtta tcaaataaag tccttttatc ctctaatgat ttattacgac
     9361 ttatgattaa ctctttaatt tctcgcaata agtcatcgta atctttaa
//
LOCUS       DBFIFS010000171         9368 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.171, whole genome shotgun sequence.
ACCESSION   DBFIFS010000171 DBFIFS010000000
VERSION     DBFIFS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.171"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..271
                     /gene="ghoS"
                     /locus_tag="N7A07_003620"
     CDS             <1..271
                     /gene="ghoS"
                     /locus_tag="N7A07_003620"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU2478210.1"
                     /translation="YVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNEN
                     SFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            299..472
                     /gene="ghoT"
                     /locus_tag="N7A07_003621"
     CDS             299..472
                     /gene="ghoT"
                     /locus_tag="N7A07_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU2478211.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(591..2108)
                     /gene="lysS"
                     /locus_tag="N7A07_003622"
     CDS             complement(591..2108)
                     /gene="lysS"
                     /locus_tag="N7A07_003622"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU2478212.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2345..3802)
                     /gene="dtpC"
                     /locus_tag="N7A07_003623"
     CDS             complement(2345..3802)
                     /gene="dtpC"
                     /locus_tag="N7A07_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU2478213.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(3861..6008)
                     /gene="cadA"
                     /locus_tag="N7A07_003624"
     CDS             complement(3861..6008)
                     /gene="cadA"
                     /locus_tag="N7A07_003624"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU2478214.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6088..7422)
                     /gene="cadB"
                     /locus_tag="N7A07_003625"
     CDS             complement(6088..7422)
                     /gene="cadB"
                     /locus_tag="N7A07_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU2478215.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7788..9326)
                     /gene="cadC"
                     /locus_tag="N7A07_003626"
     CDS             complement(7788..9326)
                     /gene="cadC"
                     /locus_tag="N7A07_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU2478216.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         2637 a         2182 c         2127 g         2422 t
ORIGIN      
        1 ttatgttgtt tcttttgatt atccatcatc ttattcctca gtgttcttaa ggttaagatc
       61 attgatgtat gatatgaatt tctcttctat cgtggctgat gaatatggga taccacgaca
      121 attgaatgaa aattccttcg caataacgac atcgttagcc gcaagtgaaa tcgaagattt
      181 aatcaggctt aaatgcttag acttaccgga tattgacttt gacctcaaca ttatgacagt
      241 tgatgactat ttccgtcagt tttacaagta gccagtaagg ataagaagaa cataaaaaat
      301 ggcactattc tctaaaatat taatttttta tgtgattggt gtgaacatat cctttgtcat
      361 tatctggttt atctcacatg agaaaacaca tattcgttta cttagtgcat tcctggtcgg
      421 aataacctgg ccaatgagtc tgcctgtggc attacttttt tctctctttt aggagctgta
      481 gttgagtgga cccgctggtt atcgtgaaac gaagcgtctc tcctcaaaac tggatttgcg
      541 gaacgatgaa cgaagcagtc gcctgactgc ttcgttcatt aaagtgaaat ttatttctgt
      601 gggcgcatcg ccgggaagag aataacgtcg cgaatagtat ggctgttagt aaacagcata
      661 atcattcggt cgataccaat acccagacca gcggttggcg gcagaccata ttccagcgca
      721 gtcacgtaat cttcgtcata gaacatggct tcgtcgtcac ctgcggcttt agcgttaacc
      781 tgttcctgga aacgttcagc ctgatcttct gcgtcgttta attcgctaaa accattaccg
      841 atttcacgac caccgatgaa gaattcaaaa cggtcggtga tttccgggtt aacatcatta
      901 cggcgtgcca gcggtgacac ttctgccgga tattccgtaa taaaggttgg ctgaatcaga
      961 tgtgcttctg ccacttcatc aaagatctcc gtgacaatac gtcccagccc ccagcttttc
     1021 tctaccgtaa taccgataga ttcagctaat gctttagcag catcaaaatt atccaggtcg
     1081 gccatatcgg tttctggacg atattttttg attgcttcgc gcatggtgag tttttcaaac
     1141 ggtttgccga aatcaaacac atgctcgcca taagtgactt tagtggtacc cagaacctct
     1201 tgtgccaggg tgcggaacag ggactctgtc agttcaatca aatcgtggta atccgcatac
     1261 gccatgtaga gttccatcat tgtgaactca ggattatggc gaacagaaat accttcatta
     1321 cggaagttac ggttgatttc gaatacccgt tcaaaaccgc ctacaaccag acgtttcaga
     1381 tacagctccg gcgcgatacg cagatacata tctaaatcca gagcattatg atgggtaata
     1441 aacgggcgag cagatgcccc acctggaatt acctgcatca tcggggtttc tacttccata
     1501 aagccgcgcg cgaccatgaa ctggcggata gcggccagaa tttttgaacg gacaacaaac
     1561 gtttgacggg atttatcgtt agcgatgagg tccagataac gttgacgata acggacttcc
     1621 tgatcctgca gaccatggaa tttatctggt aaaggacgta gtgctttagt cagcaggcgc
     1681 aactcagtac agtgaatgga aagctcaccc gtttgcgtct taaacagcgt accgcgggca
     1741 ccgataatgt cacccagatc ccatttttta aactgatcgt tataaacacc ttctggcagg
     1801 ctatctcgtg cgacgtacag ttgaatacgg ccaccgacat cctgcaacgt tacaaaggag
     1861 gctttcccca tgatacgacg ggtcatcatt cggccagcaa ccgagacttc aatgtttaag
     1921 gattccagtt cctggttatc cttcgcatca aactcttcgt gcaactggtc agaggtatgg
     1981 tcgcggcgaa aatcattggg aaacgccaca ccttgctgac gtagtgccgc cagtttttcg
     2041 cggcgatttc tcagttcatc gttaaaatca atagcctcat tggctccccg tgtttcttgt
     2101 tcagacattt tggttcctct aaatccagct ttcaatttag cgtagataaa gagacagatc
     2161 ggtacactgt attatctgcc tcgactatta ataactcagt ttatcttatc gaaactaacg
     2221 cttacctcaa gttgatatca ctaataaata accatcaaaa tggtttaaat gacttattaa
     2281 tctcgattta ctagtgtgat aaactgaaag ttaatttatt cataaccaat tgtttttacg
     2341 acagttaatc gttgctctcc tgtaccatat tcgtcggcgt gctaaacagg aaacgggtag
     2401 caaaggccaa taccacgatg atcgcgacac aagccaacgt ccactctccc atctgagaaa
     2461 agaatcgctg gtaagctgca attgccatac cgctaatttg cgactctgtc gtctgctgtg
     2521 ccactacgcc tgccagccag ttggcgaccg cgcctgttgc cagcatataa atcccggtta
     2581 atacgccaga cattttcaga cgcgtgattt gcgcaatcgc caccgggtca ataaagagtt
     2641 cggcaaagcc cattagcgcc agcccggata ccatcacgcc cattgacgct tgaccgtcag
     2701 ctgctgcatg tcgggcatca aatgccagca acataaagcc acaagccatc agcagtaagc
     2761 caaaggcaaa cttcagccag acgcgcaatg ttgagttgcc gtggctttct ggcgacgcca
     2821 gccaggccag aacaacccca gcgagcatca ccgcaattgc attcaccgac tggaatagtg
     2881 cagtaggtac ttcaatattg aatgcctgac gattcacaaa gcgatcgata aacaagctga
     2941 tggtactgcc gccctgttgt gccagtaccc agaacaatgt cccgacaaac atcaacaata
     3001 caatttgcca aagagcacgg cggtgttcgg ggaatttgat catcatgcgg gcaatgattt
     3061 gtgcggcaat gaggcaaacg atcgccagca aatatccgga ccagtcgttc tccagcagca
     3121 gggtaaaaaa tactggggct aaacagagca tcaccactaa ccagctccat accggtaagg
     3181 caaatttgac gctagtgagc gcttttttat ccatactacg cgtggactgg aaatgacgat
     3241 gcccgcttaa gaaaatcaac aaaccgataa acatgccgcc acccgcaagg gcaaagccaa
     3301 catgccatcc ataccactga gcagccaggc cgcaggcgat tggggcggcg atagaaccga
     3361 tattgcccgc agcatacagc agcgaaaaac cgccatcacg tcgatgatcg ttctcgtcgt
     3421 agagctcgcc aagcaaacag ctgatgtttg atttgaataa accgtagcca caaataatga
     3481 ttgccagcgc cagatacagg ctaaaggttg aatttgtatc aatacccagc accacatggc
     3541 caagggtcat taacagcgcg ccggcaatca ctgcagtgcg gttgccgagc aggcggtcgg
     3601 caagccagcc gccgagaata ggggtaacgt aaaccagaga agcatatgcg ctgaacaggc
     3661 tgatggcatg gttatcatca aaaccaagct gatgggtgag atagagaatg agtaaggcac
     3721 gcatgccgta aaaactgaag tactcccaga tttggatcgc cacgatatag tatatcgcgc
     3781 gcggctgtga gggtgttttc atgtgttctc cttatgagca aaaaagggaa gtggcaagcc
     3841 acttcccttg tacgagctaa ttattttttg ctttcttctt tcaatacctt aacggtatag
     3901 cggccatcag cctgacggta tgcaccgtga atatcggttt caaagcccgg atagtgagcg
     3961 ccgatttcac acagcatctg caggaactcc agaaccggac ggctttcttc ggtgatcatt
     4021 tcacccggca ttaccagagg aactcccggc ggatacggaa ggatcatatt ggcgttaata
     4081 cgaccgacca tttcgtcgag gtaaacttct tcggtcatac cgtgcagctc tttctggaat
     4141 gcagcatacg gagtcattac catcgtcggc agcacttcaa atgcgcgata catcagatcc
     4201 ggcagattgt ggtgaacaat cagtttgtgg atattctgag ccagttcctg aatacgcatg
     4261 ttttcataga attcaggatc ttcacgatac agagacggca gcatgttttt cacacgcagg
     4321 ttcaggtcga acgcacgttt gaagtcagtc agagcacgca gcaggctcag tgctttggtc
     4381 ttatcgatac cgatgctgaa caggaacagc aggttatacg gaccggtttt ctcaacaacg
     4441 atgccatgtt cgtcgaggta tttcgccacg atgctggccg gaataccaaa gtcgctcatg
     4501 gtgccgtctt tttccatccc cggagtcagc agggtgactt tgatcgggtc aagatacatg
     4561 tgctcgttat cgatgttttt gaagccgtgc caggtgctgt cagaacgcag cggccagcat
     4621 tcagtcgtat cgatatgatc cggctgccat acatcaaaga accagccatc agattccgtt
     4681 ctcagacgtt tgatctcttt acggaatttg atcgcacgtt caatggaacc gttgatcaga
     4741 cgcttacctg cattgccttt catcatcgcc gcagcggttt cagtggacgc cacgataccg
     4801 tagtgcggag aagtggtggt gtgcatcatg taggcttcgt taaaggtttc ttcgtttacg
     4861 tcacctttaa cgtggatcat ggaagcctga gagaacgccg ccagcagttt gtgagtggac
     4921 tgggtttcgt aaatcacttt cccttctaca cggccaccgc tcataccgca tttaccttcg
     4981 taaatcggtg agaagttggt gtaaggcacc cacgcggagt caaagtggat ggatttcaca
     5041 tccagtgttt tcttgatgaa gtcggtgttg tacagcagac catcataggt agagttggta
     5101 attacagcat gtaccggcca ggttgcgttt ggtgtttctt tcacgcgctt agcaatggta
     5161 gcgtgctgga attcactctg tgggatacca ccaagaatac cgtaagcgtt acgggtcggg
     5221 cggaaataga ttggcgtaac atcgctcatc atcatcaggt gggtcagcga tttgtggcag
     5281 ttacggtcaa tcagaatggt gctgcctgcc ggagcagagt acataccaac aattttgttc
     5341 gcagtggaag taccgttggt caccatgtag ctgcggtctg cgttaaagac gcgagcgata
     5401 tactgttctg cttctttgtg tggaccactg tgatccagca gagaacccag ttcagatact
     5461 gaaatggaaa tatcagattt catggtattc ggaccaaaga aatcatagaa caggctacct
     5521 accgggcttt tctggaatgc agtaccaccc atgtgaccag gagtacagaa agtatattta
     5581 ccttcacgaa catatttaaa cagtgcttta gtcagcggag gcagaatagt gttgatatat
     5641 tcgtcagtgg tctgcttgat cttgttagca atatcttcag cagcacccag cgcatattca
     5701 aagaagctaa tctgtaaacg caggtcattc aggcttacat cgagagtgga atacgtatta
     5761 gcgaacgcgt acaacggcag gttctcgttc attttgctaa tttcttcgca cagctcgaga
     5821 ttatatttat cccagtcaaa aataacgccg cacagacgcg cattgttttc gatcagtttt
     5881 aataagtcgt cacggtcgtt cgggtaaaca atctggaagt tcagacgttc aagcgcgcga
     5941 tgaagttcac ggatgggttc ttctttaaaa taaaccccca tgtgattcaa tattgcaata
     6001 acgttcatag tcatatctcc aggtaaaaaa ggcccctccc aacacatggg acaaaatgaa
     6061 aggaggagcc tcggaaaata cttttaatta atgtgcgtta gacgcggtgt ggttatccat
     6121 tgagtggctc tggcgctcgt gcattttgcg agcgtagaac atcaggataa tcaggctgac
     6181 gatgaaggta cctgccagct cgaaggagct tgcgcccatc agcgcgatga agcagaacac
     6241 gcaacccagt acagagcaga tcaggctgac aaagttgcgg atgttaacgc cttcaaaacg
     6301 aatcaggtca acgcaagagt agaagtacgg cagcatagtc agcagtactg cgataccggt
     6361 cagttcaccg aacaggtcag atgctttacc accggcagag ttcatcagag tgatgaggat
     6421 catcagggca gtcattttca ctgcagccag cagcagacct tttttcggaa taccgttgct
     6481 gtcgacttca ccataaactt tcgggaagtt accgtcgtta gcggcacgta cacctgcctg
     6541 gcctaccaac atcatccagg agcccagaga agtcaggcac gcaaaggcgg tgaatgcaga
     6601 aaccagcggc gcagcccagt taccgaggat agttgaagca ctgattgcaa acggagcacc
     6661 ggaagccgcc attacagaag acggatacat accggaaagc acctgagtcg cagcgatgta
     6721 aacaatacct gctaaaccag tacccagcat ggttgccagc ggaacggtac gtttcgggtt
     6781 tttaaccata ccagtactta cagctgcgga ttcaacaccc acgaaggccc acaggcagag
     6841 cagaatactt ttaatgatcg catgaccatc agtggtatcc gcagtattcc agttagctgc
     6901 ataagttgcc gcatcaaacc aatgccagcc aacaatagca gtcatcacca caggaataag
     6961 aaccagcacc agaccaatag tggttaaacg gcttacccag gtaccgccga gcatatttac
     7021 aaaggtaaat acccagacga tagcaataca ggcgataccc gccggaacag gatcatttaa
     7081 tactgggaag aaggtggaaa gataagatac agcggtaata ccaatcgcca ggttaccaat
     7141 ccagttagca tggtaataaa gaacacctgt ctgaaaacca aatgcaggag aaatttctcc
     7201 ggcataagca attgggccac cttgttgcgg gttttttgtt gccagtcggg catatacata
     7261 cgccagcgac attgcaccaa taatagagat aatccaaccc cagatagcaa taccaccgat
     7321 acttgctagg ttcgcaggta ataatgcaat accgctcccc atcatattac cggcaacaac
     7381 accggtacag gcaaatagcc cgatcttctt ggcagaactc atgctcttct cctaatttca
     7441 tttttgaatt tggagtccgg gtcatgatgt ataactattt cctgaccaga ccaaactggc
     7501 gctaagatta ctcacgaaaa aaggattaat cctaaagatt aggtgaaata acacaaaagt
     7561 ttctgtaagt gagaacttga ggttttttat taacacatca ggatcgcaag ttgatatcat
     7621 gaaaagataa acattcaatg tttacaatag attgcgtgac gtcttctggt taaatttatg
     7681 taataaaaat tatgcggcaa ataaattgcc gcaacatatt ataccaacag gaacatacaa
     7741 aaactcaaca acaaatattc ccgagcatga atcaacccgg gggttactta ttctgaagcg
     7801 agaaatttgt cgagataagg tacaacataa ggaacagaag tctggaatat accattttca
     7861 atccagtaaa gggtgtttgc ccctgggcgt aaattaaagg cggtgagata tgcatcagcc
     7921 gcttcccggt tcatcccctt catttcataa accttgccaa gcaatacata atttagccag
     7981 gacatttcaa gatcaatgcc agtatttatc gcctggtaag actcatctgt tttacctttt
     8041 accagagcac tgaccgcttt tatttgatat ataatggaca ggttattcaa ttccggtagt
     8101 gtaacaatgt tatctatttc tgtgttcagt gctgctaatt gtttttcatc taaaggatgt
     8161 tgagaatggc gcacgatatc aactaatgct ttttctgctc tcgcgtaggt aaattctggg
     8221 gatgattgaa caatctcacc taataattca ctggcacggt tcaatgattt atcatcgcca
     8281 tgcagtaaat aatcatgtgc ctgataaaaa ttagttaata acgcaccacg atgaggcaaa
     8341 atttgctgga gcgtctcctg cattcgttgt ggccacggtt ggtttaacgc ttttgataaa
     8401 ctctccagta aatcattttg aatcgccagc tgattaccgt tagtgatgac ataacgttta
     8461 tccagcatgg ttgaaccatc tgcattgtct accaatttta tcgacataaa gcattgttga
     8521 gcacggtatt ggcgctgatt aacaaacgca atagataatg ttttaccgga actgctcggt
     8581 tcatcaatgt tgtagttgat tttgtcatgc accataaagg tggagaaggt gttaagtgat
     8641 gtcgccacca aatcacccac gcctatcgcg taagagagct gatacgggga actccagctg
     8701 ttacaacttt tatttaccat attaatgtca atatcgcgtg gattgagcaa aatacgcgat
     8761 ttgctcatcg gaagacgtgt atcaagactt gaaaacgcta ccagtgctac acagatacct
     8821 aaggacaaca ggaaaaaaaa ccatacccaa aaggtagtga atcgtttgct tttaactggg
     8881 gattgttcag gtggcgttgc ggtgttttga atgttaagac tgtgggaggg agaatctgtg
     8941 gcaggaaccg cctctggtat agggggaggc gaagatagca ttatttcctc tccctcttct
     9001 tcgctgtacc agataaccgg caccattaat ttatagccgc gctttggtac agtagcgata
     9061 tagacaggac tatcttcatc attatctttt aatgacttac gtagttctga gatactctgc
     9121 gtcacaacgt gattggtgac aatacttctc ttccagacat tatcgataag ttcatccctg
     9181 ctaagtactt cgccactgtg ttgagcaaag aaaaccagaa ggtcgattaa tctcggctca
     9241 agggtaagtt gacgcccatt gcggctaatt tggtttatgg acggagtaac aagccattcg
     9301 ccaacgcgaa ctacaggttg ttgcataata gaaactcatt cgaaaaggga atgatgcaat
     9361 gataattg
//
LOCUS       DBFIFS010000172         9198 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.172, whole genome shotgun sequence.
ACCESSION   DBFIFS010000172 DBFIFS010000000
VERSION     DBFIFS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.172"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            115..477
                     /locus_tag="N7A07_003627"
     CDS             115..477
                     /locus_tag="N7A07_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478217.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(656..1114)
                     /gene="ybbJ"
                     /locus_tag="N7A07_003628"
     CDS             complement(656..1114)
                     /gene="ybbJ"
                     /locus_tag="N7A07_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU2478218.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1111..2028)
                     /gene="qmcA"
                     /locus_tag="N7A07_003629"
     CDS             complement(1111..2028)
                     /gene="qmcA"
                     /locus_tag="N7A07_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU2478219.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2174..2851
                     /gene="fetA"
                     /locus_tag="N7A07_003630"
     CDS             2174..2851
                     /gene="fetA"
                     /locus_tag="N7A07_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU2478220.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2838..3617
                     /gene="fetB"
                     /locus_tag="N7A07_003631"
     CDS             2838..3617
                     /gene="fetB"
                     /locus_tag="N7A07_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU2478221.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3680..4534)
                     /gene="cnoX"
                     /locus_tag="N7A07_003632"
     CDS             complement(3680..4534)
                     /gene="cnoX"
                     /locus_tag="N7A07_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU2478222.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4595..5404)
                     /gene="ybbO"
                     /locus_tag="N7A07_003633"
     CDS             complement(4595..5404)
                     /gene="ybbO"
                     /locus_tag="N7A07_003633"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU2478223.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5394..6017)
                     /gene="tesA"
                     /locus_tag="N7A07_003634"
     CDS             complement(5394..6017)
                     /gene="tesA"
                     /locus_tag="N7A07_003634"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU2478224.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5988..6674
                     /gene="ybbA"
                     /locus_tag="N7A07_003635"
     CDS             5988..6674
                     /gene="ybbA"
                     /locus_tag="N7A07_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU2478225.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6671..9085
                     /gene="ybbP"
                     /locus_tag="N7A07_003636"
     CDS             6671..9085
                     /gene="ybbP"
                     /locus_tag="N7A07_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU2478226.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2089 a         2370 c         2532 g         2207 t
ORIGIN      
        1 cgaaataaat tctgtttctg gactaaacgt gaagcggttc ctgatgtgtg ctaaaatgcg
       61 tttctttgat aaatctcaat ttttaagagg tttacgccag gcaggaaaat ttaaatgaat
      121 gaatttgaga agatttttaa tgaaatgaat ctggaccggg cattgctacc gatccttttt
      181 cgttcgaacc ggtcgacagt gtggaagtat ttaagcggcg atagtacggc tcctgcctct
      241 gccatgtcgc tgattatgct gttgcagttg atacagaaac gaaaccccga tctgttagcc
      301 gaatggttaa cgctttctga tttcaccatt ccgccagaag tttacctgga ccagcctgat
      361 tactggaaag ggtgggtgta tacgcaacac aaggtcaata aaaacgtcct ggaatatcta
      421 aaaaaacact atccggatga agaccaaaaa agtatgagta aaggccggga agagtaaatt
      481 gccaatgcca gttaacattt aacatggcgg aggcggccta cggttttggc gtgatatgac
      541 gcgcccgcgt cgcatcaggc gtcggtgcac gactgccgga tgcggcgtaa acgccttatc
      601 cggcctacga ttcgggcgca gacttccggg catgataaga cgcgtcagcg tcgcatcagg
      661 cgatgaccgc acggatgatc agcgttatcc cttctatcgc aatgacttca acatgcgtac
      721 ctgcgccgag atcctcgctg gcgctgacag gccatgaact gtcaccgacg cgcatatgac
      781 cgcgcccgtt gaccagcgga gattccagca caaaacgtcg gccaatcagc tgctgcccgc
      841 gctggtttaa atgactgtcg ctgtgctttt gttcgcgcac ccgccgcgac aaccatttcc
      901 accacagcca ggcggcgagc agcgtcagga cggcaaacat caccccttgc cactcccaac
      961 ccagcggcac cagccagacc accaggccag taatcactgc tgccacgcca ctccacaaca
     1021 aataaccatt tccgcccagc atctcggctg ccagcagcaa accgccgaga ctgagccaga
     1081 aaatatgtgg atgaacgact attaactcca tcatggctga gtccgcttgt tggcgctgtc
     1141 tttcaccagc tcggcaatcc cggcaatcga ccccatcagg ctgctggcct ctaatggcat
     1201 catcactact ttgctgttac tggaggaacc gatctgctgt aacgcttcgg tgtatttctg
     1261 cgctacgaag tagttcaccg cctgaatatc accggaggcg atggcttcag acaccatttt
     1321 ggtggcgcgg gcttctgctt cagcggaacg ttcacgcgct tcagcctgta aaaacgccga
     1381 ctgacgttcg ccttccgctt tcaggatttg cgactgtttt tcaccttcgg ctttgaggat
     1441 ttccgcctga cggatccctt ccgcttcaag aatgtaagcg cgtttggtac gttccgcttt
     1501 catctgcgcg ttcattgaag agataagctc tgccggtggg cgcacgtcgc gaatttcaat
     1561 acgggtgact ttaatccccc acgggttggt ggcctcatcg acaatacgca gcaggcgtga
     1621 gttgatgctg tcgcgctgag agagcatttc gtcaagttcc attgaaccca acacggtacg
     1681 gatgttagtc atggtcaggt tgatgatcgc cagctccaga ttgctgactt cataagccgc
     1741 gcgtggcgcg tcaatcacct gaataaaaca gactgcgtcg atggtaacgt tggcgttatc
     1801 tttcgagata acttcctggg aagggatatc gagcacttgc tccatcatat tgatcttgcg
     1861 accaatgcga tccataaacg gcaccaccag actgagcccc ggctgtaacg ttttggtata
     1921 gcgaccaaaa cgttctaccg tccattgata gccctgcggc acgattttga cgcccgcgcc
     1981 gacaatgacc agcgcgacaa aaatgagaat cgggataaag ataagcatcg gaaaaacctc
     2041 ctgttgtacc gtccataatc agcaaaattg ctgcttgatt aaacaaatta tacctgatta
     2101 ctgaaagaga gttccccctt attcctgcga aggataaact gtttttagta aaaatcagaa
     2161 aaagggaaca gcgatgcagg aaaatagtcc tttgcttcag ctacaaaacg taggatatct
     2221 ggcgggtgat gcgaagattc ttaataacat caatttttcg ctgcgtgctg gcgaatttaa
     2281 gttaattacc ggtccttctg gttgtggcaa aagtacgctg ctaaaaatag ttgcttcatt
     2341 gatcagccca accagcggaa cgttactgtt tgaaggtgag gatgtcagca cactaaagcc
     2401 agaaatctac cgtcagcaag tctcttactg cgcccagacg ccgacgctgt ttggcgatac
     2461 ggtatacgat aatctgatct ttccctggca gatccgtaac cagcagcctg acccggccat
     2521 ttttctcgat tttcttgaac gcttcgcctt gccggacagc attttgacga agaatatcgc
     2581 cgagctatct ggtggtgaaa aacaacgcat ctcattgatt cgtaacctgc aatttatgcc
     2641 gaaggtttta ttgctggatg aaatcaccag tgcgctggat gaaagtaata aacataacgt
     2701 taatgagatg atccatcgtt atgtgcgcga gcaaaatatt gccgtgctgt gggtgacaca
     2761 cgataaagac gaaattaatc atgcggataa agtgattaca ctgcaaccgc atgccggaga
     2821 aatgcaggaa gcacgctatg aactcgcata atattactaa cgaatcatta gcactggcat
     2881 taatgctggt agtggtggca atcttaatta gccataaaga aaaactggcg ctggagaaag
     2941 atattctctg gagcgtcggg cgcgcgataa ttcagctgat tattgttggc tatgtgctga
     3001 agtatatttt cagcgtggat gatgccagcc tgacattatt gatggtgtta tttatctgct
     3061 ttaatgcggc gtggaacgcg caaaaacgca gtaaatatat tgctaaagct tttatctcat
     3121 cgtttattgc cattacggtc ggggcgggaa ttaccctggc ggtgctgatt ctctcagggt
     3181 cgattgaatt tatcccgatg caggtgatcc ctatcgccgg gatgattgcc ggtaacgcca
     3241 tggtagcggt ggggttgtgt tacaacaatt tagggcaacg ggtcattagc gaacagcaac
     3301 agattcagga gaaactgagt cttggcgcga cgccgaagca ggcttcagcg atattgattc
     3361 gcgacagtat tcgcgcggct ttaattccga cggtcgattc agcaaaaacg gttggcttag
     3421 tgagtttacc aggaatgatg tccgggctga tatttgccgg gattgatccg gtgaaggcga
     3481 ttaaatatca gattatggtg acctttatgc tgctctcaac cgccagcttg tcgaccataa
     3541 ttgcctgcta tttaacctat cgtaagtttt ataattcgcg ccaccagttg gtggtgacgc
     3601 aattgaagaa gaaatgatgc gatgccggat gcgcagcatc gcatccggcg ttgtggttca
     3661 tgtgccgatc aacggaatat caatacaaca atgcatacag ctggcggcga tacttcgacg
     3721 ccagtgcatc acccgtaccc agcgcagcga ggatctcctg gaacgtttta cgcgtctgac
     3781 cgtctgcggc ggtgagatct ttacgcagat gcccgaacag caactccagc gcctcttcat
     3841 tgcgcccaac ctgatgcagt tgcagcgcca gttgcgtcgc cagtgcggca tcttctggat
     3901 tctccgccac ctgctgttgc aactgttgaa tttccggcgt atcagccgcc tgcttcagca
     3961 gttcgatttg cgccaccagc ccctggtagc gggtgtcctg atcctgcaac ggaatggttt
     4021 tcagcaccgc ttccgcatct tcagaacggt tcagcgcaat cagcgtttct gccagcagca
     4081 ggccgatctc cccgttctga ttcgacaact gccaggcgtc tttcagcaat ggcagggcat
     4141 cggtgtaatt gccttcctgc atcagttgca tcgcctgctg cgctttcagc tcttcttcgc
     4201 gcggcagcac tttatccagc agggcgcgga tcgcctcttc cggttgcggc ccctggaagc
     4261 catctaccgg ttgcccgttc tggaacagat acacggtcgg aatcgcgcgc agaccaaact
     4321 gggcggcaat catctgctcc gcgtcgcagt ccagcttcgc cagaataaat tgcccgttgt
     4381 actgcgccgc aaggctttcc agaattgggg ttaactgcaa acagtgctgg ctacgttcag
     4441 accaaaaata gaacagcacc ggagtggtca tcgactgttc aagaacctgt tgcaggttag
     4501 attcgttaat gttgacaata ttttctacgg acatggagtc gctctctgtt gtcgatattt
     4561 tctttgacat gggggcttaa gcgcgcgctt caactcaccc ctgcaatatt ttgtccatca
     4621 cgcgccccgg cagcaggcgc ttaagcacca ttaccgccca ggtcaccagc gttaccggat
     4681 agcgcatctt cggcttctcg ctaataaaag catggcgtac tttgtccacc accgcttccg
     4741 gtcccaacgt aaagcgggcg gcgatgccgg gattttcgac tggtttatca ctttgcgtct
     4801 ggttgacgtt gtcggtgaag cgagtacgaa tgggaccggg ttcgatcagg ctgactttaa
     4861 ttccgctgcg gcgcagctcc atgcgcagcg cgtctgacca cgcctccagc gcatatttac
     4921 tggccgcgta agcgccacga cccggtgtgg agattaatcc catcaccgat gaggtcatca
     4981 caatacgccc ttcaccgtgc ggcaacatcg cgggtaacag gcgcatggtg agctggtgtg
     5041 cgccgaaaaa gttggcggaa aactgctgtt ccatctgcgc acggctgatg gtggaaaggg
     5101 ggccatacat gccgaatccg gcattgttaa agatcccata cagacaatta tcggtcaggg
     5161 cgatcacctc gtcggctgcg cgatcaacac tttctggtga atccagatcg atcaacacgc
     5221 cggtaaatcc catgctgttc atgcgctcaa catcatccgg tttccggcaa cctgccagca
     5281 catgaaaacc ctggcgtttt aattcgagcg cgctttccag gccaattcca ctggaacatc
     5341 cggtaattaa gaccgatttt tgcataactt tacctgtcag gatctccgtt gctttatgag
     5401 tcatgattta ctaaaggctg caactgcttc gccatccagt cggcaataaa cggctgggcg
     5461 tcgcggttgg gatgaatacc gtcatcctgc atccattgtg gcttgaggta gacctcttcc
     5521 attaaaaagg gcagcagcgg aacatcaaac tctttggcga gtttggggta aatggcgcta
     5581 aaggcttcat tataacggcg accatagttt gcaggcagac gtatttgcat taacaatggt
     5641 tcagcgttgg cggctttgac atcctgcaaa atctggcgca gcgtttgctc ggtttgctgt
     5701 ggctgaaaac cacgcaaacc gtcattgccg cccagttcaa ccagcaccca acgcggctga
     5761 tgctgtttca gcagagccgg aaggcgcgcc agcccttgtt gcgaggtgtc gccgctgatg
     5821 ctggcattga ctaccgacgt tttactctgc cacttatcat tcaacaaggc aggccaggcc
     5881 gcgctggcag acattcgata cccggcgctc aggctatcac ccagaatcaa taacgtgtcc
     5941 gctgcggcag cacggaaggt taacaggacc agaaacagga agggcaaatg ccagcggaaa
     6001 acattgttga agttcatcat cttaagaagt ccgtcggtca gggggagcat gaactctcca
     6061 tcctcaccgg agttgagctg gttgtcaaac gtggcgagac catcgcactg gtgggcgagt
     6121 cgggatcggg taagtcaacc ttgctggcga tcctcgccgg gctggatgac ggcagcagtg
     6181 gcgaagtgag tctggtggga caaccgctac ataatatgga cgaagaagcg cgggcaaagt
     6241 tgcgcgcgaa gcacgtcggc tttgtttttc agtcatttat gttaattcct acccttaacg
     6301 cgctggaaaa cgtcgagctt ccggcgctgc tgcgcggtga gagtagcgcg gaaagtcgta
     6361 acggagcgaa agcgttgctc gaacagttag ggctgggtaa acgtctggat catcttccgg
     6421 cacagctttc cggcggtgaa cagcaacgag tggcgctggc acgagccttt aacggtcgac
     6481 ctgatgtgct gtttgccgac gaacccaccg gtaaccttga ccgccagacg ggcgataaaa
     6541 ttgccgacct gctgttttcc ctcaaccgtg agcatggcac cacgttgatt atggtgaccc
     6601 acgacctgca actggcggca cgctgcgacc gctgcttacg gctggtgaac gggcagttgc
     6661 aggaggaagc atgattgcac gctggttctg gcgcgaatgg cgctcgccgt cgctattaat
     6721 tgtctggctg gcgctaagcc tggcggtggc ctgcgtgctg gcgctgggca atatcagcga
     6781 tcgcatggag aagggcttaa gccaacaaag ccgggagttt atggcgggcg atcgggcgtt
     6841 gcgcagttcg cgcgaagtgc cgcaagcgtg gctggaggaa gcgcaaaagc gcggcctgaa
     6901 agtcggcaag cagctgactt tcgccaccat gacatttgca ggcgacacac cgcagctggc
     6961 gaacgttaaa gcggtggatg atatctaccc gatgtatggc gatctgcaaa ccaatccccc
     7021 tggcctgaaa ccgcaggcgg gcagcgtatt gctggcccca cgcctgatgg cactgcttaa
     7081 cctgaaaacg ggcgacacca ttgacgtggg cgatgccacc ttgcggattg ccggagaagt
     7141 gattcaggaa ccggattccg gttttaaccc cttccagatg gctccgcgtc tgatgatgaa
     7201 tctggcggat gtcgataaaa ccggagccgt gcagccgggg agtcgggtca cctggcgcta
     7261 taaattcggc ggcagcgaga accagctcga cagctatgag aaatggttgt tacctcagct
     7321 taaacccgaa caacgctggt acggtctgga acaggacgaa ggcgcgctgg ggcgatcgat
     7381 ggaacgctcg caacagttcc tgctgctttc ggcacttctg accttgctgc tggcagtggc
     7441 agcagtggcg gtagcgatga atcattactg tcgcagtcgc tacgatctgg tggcgatcct
     7501 caaaacgctg ggggcagggc gagcgcaact gcgtaagcta atcgtcggtc agtggttgat
     7561 ggtgctgacg ctttcagccg ttaccggtgg ggcgataggc ctgttgttcg aaaacgtgtt
     7621 gatggtgctg ctcaagcccg ttctgcctgc tgcactaccg ccagccagcc tctggccatg
     7681 gctatgggcg ctgggcacca tgacggtcat ctcgctgctg gtggggctac gaccataccg
     7741 cttgttgctg gcaacgcagc ctttacgcgt attacgtaat tatgtggttg cgaacgtctg
     7801 gccgctgaag ttttatctgc cgattgtcag tgtggtggtt gtgctgctgc tcgccggatt
     7861 aatgggtggc agcatgctgc tttgggcggt gctggcgggc gcggtagtac tggctttgct
     7921 gtgcggtgta ctgggctgga tgctgctgaa tgtacttcgc cgcatgacgc tgaaatcgct
     7981 gcctctgcgc ctggcggtta gccgcctgtt acgtcagccg tggtcaacgt taagccagct
     8041 ttcggcattt tcgctctcct ttatgctgct ggcactgcta ctggtgctgc gtggcgatct
     8101 gctcgaccgc tggcagcagc agttaccgcc agaaagcccg aactactttt taatcaacat
     8161 cgccacagaa caggttacgc cgctaaaagc gttcctcgcg gaacatcaga tagtcccgga
     8221 atcgttttat ccggtggtgc gagcgcggct gacggcgatt aacggcaagc cgacagaagg
     8281 caatgaagat gaggcgctca accgcgaact caatcttacc tggcaaaata cgcggcccga
     8341 tcataatccg attgtcgccg gtaactggcc gccaaaagct gatgaagttt cgatggaaga
     8401 ggggctggcg aaacgcttaa acgttaccct cggcgatacc gtgactttta tgggcgatac
     8461 ccaggagttc cgcgctaaag tgaccagcct gcgcaaagtg gactgggaaa gtctgcggcc
     8521 taatttctat tttattttcc ctgaaggggc attagacggg caaccgcaga gctggcttac
     8581 cagtttccgc tgggagaatg gcaacggcat gttgacgcaa ctcaaccgcc agttccctac
     8641 cattagcctg ttagacattg gcgcgatttt aaaacaggtc ggtcaggtgc tggagcaggt
     8701 aagtcgggcg ctggaggtga tggtggtgct ggtcaccgcc tgcggtatgt tgctgttgct
     8761 ggcacaggtg caggtgggaa tgcgtcagcg tcatcaggag ctggtggtgt ggcgcacact
     8821 cggtgcgggg aaaaaactgc tgcgtaccac gttgtggtgt gagttcgcca tgcttgggtt
     8881 tgtttccggc ctggtggccg caattggtgc ggaaacggca ctggcagtgt tgcaggcgaa
     8941 agtgtttgat tttccgtggg agccagactg gcgattgtgg attgttctgc cgtgcagcgg
     9001 tgcgctgctg ctgtcgcttt tcggcggctg gctgggcgcg agactggtta agggtaaggc
     9061 gctgttcagg cagtttgcgg ggtgatgaga gtaaataagg ggcgttctgg ttgaatggaa
     9121 cgccttgttt aaattaattc tacaatatgt ttcattgatg atgttattga attggtgatt
     9181 tcctatcttt ctattgct
//
LOCUS       DBFIFS010000173         9190 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.173, whole genome shotgun sequence.
ACCESSION   DBFIFS010000173 DBFIFS010000000
VERSION     DBFIFS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.173"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(118..441)
                     /locus_tag="N7A07_003637"
     CDS             complement(118..441)
                     /locus_tag="N7A07_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU2478227.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(449..634)
                     /locus_tag="N7A07_003638"
     CDS             complement(449..634)
                     /locus_tag="N7A07_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU2478228.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(631..3270)
                     /locus_tag="N7A07_003639"
     CDS             complement(631..3270)
                     /locus_tag="N7A07_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU2478229.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3478..4467
                     /gene="ldhA"
                     /locus_tag="N7A07_003640"
     CDS             3478..4467
                     /gene="ldhA"
                     /locus_tag="N7A07_003640"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU2478230.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4578..5000
                     /gene="hslJ"
                     /locus_tag="N7A07_003641"
     CDS             4578..5000
                     /gene="hslJ"
                     /locus_tag="N7A07_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU2478231.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4997..5263)
                     /locus_tag="N7A07_003642"
     CDS             complement(4997..5263)
                     /locus_tag="N7A07_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU2478232.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5537..9061
                     /gene="nifJ"
                     /locus_tag="N7A07_003643"
     CDS             5537..9061
                     /gene="nifJ"
                     /locus_tag="N7A07_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU2478233.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2287 a         2406 c         2262 g         2235 t
ORIGIN      
        1 gggaaggata tataaggttt ttgtagaaat acccacggta attaagcatg ctaacgtcag
       61 tttaaatact gtaaaagatc attacattaa tagatacatt ttgtagccaa ctataattta
      121 tttgcgcaac catttaccat taactccctg aacatattgc cccgacttcg cgcgagcaac
      181 aagcttttgt ccagccagtt tggctatctc tttagtggat acattatttt gctttgccag
      241 ttgttggtaa ctatggttac gagcttcatt aatatctttt actaatgcct gtgtttcggc
      301 atccgtttgt agcgccacca ggtaaccgtt aagcgtttca cctacccgcc cctgacttct
      361 ggcttcgttc agggttaaag ccattgcact gtcgctcatc aggccaatgg cccatacagc
      421 tatgagtaat gttcttttca tcatcacctc aaaatagatc gctacgggtt ttgagcaggt
      481 cttcaacgtc cttatccgct ttaatgatga tttcatgctc aattttaacg ttcatattaa
      541 tggtgattgg ctctttcggc gcagccactt caatgcgagg cgtgcaacca accaacataa
      601 aagaggacgc aaacgcggct atcactattt tcattgtttt tcctcacact cttttcctga
      661 tgggcagcca gtttccggta gctgggcatt ctgctccagc catacctgga gattatcgcc
      721 aaagcgtaaa ctgcgccata acgtaaaaat attctcttca tggtgataat taaggtttac
      781 cgtaccgctt ttaccatcga cgcgactggt gcccgaaatg ttggcctgca tggttaacac
      841 accgagatta tctaaattaa tccttgttga tgagcgactg atttccatat aacgcaacca
      901 gttgatggca gttccagcgg tcatattgtc tttggccacc gcatctgcag tgtctttatc
      961 cagacgcagc gtcatcggcc ccgcgtttgc caaccagcca tctttgacaa tccatttttc
     1021 attgttcagc catagtggca acgccccact aaatgcccca gacatagcaa actgtttcgg
     1081 attaaccgcg ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc
     1141 atgttgcggc atacgtaatt gtttcatcag cacgttaccg cctaacacat cgacgctaac
     1201 atcagtcaac agcaatggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt
     1261 aatatttttc gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg
     1321 agtccccaga tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt
     1381 aacctgatta tctggcatcc aggcactacc gcctttcaac acgccgtgcc ctcccgcgcg
     1441 gaatccttgt tcaggcgcag cagaaaatgc aacctgagca tacagttcac catcgcgtaa
     1501 gttcatcttc cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gtttaggcca
     1561 ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat
     1621 ctcaccagca tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct
     1681 aaattttaag gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc
     1741 cagtgacagc gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt
     1801 gtcgagaatt aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc
     1861 ggtggagaga tcggtcagcg taatcgtgct gtcatgccac tcaccttttc ctgcgacatc
     1921 ccaggtggca ttcatcggtg taaaactccc ttttccccag tagcgccact gccagcggcc
     1981 agcgtcaggg agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc
     2041 attttcatgc gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt
     2101 tacacccgct aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt
     2161 tgaacgaagt aacgcgcctg gctcaaaggt cagcgttggg tcagacagac ttccacttag
     2221 ctgcgcaggt aaacgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg
     2281 cagaggcagc tggctgttat ccatgcttaa ttttcctggg ccaaaattaa gcaccgcgtt
     2341 gcccttaccc gcttgcccct gggtcagcac actcagtcgt ccgctgacca gagcgttctc
     2401 aagccctgcc tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc
     2461 atacggccag ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg
     2521 caaatcgagc aacggatcgc cgttatcccg tgccagcacg atcaattgcc cgctattttc
     2581 ctgccagtcc agctcggcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt
     2641 agcatgccca cttacaggaa gtccatccgg catgagcggc atagtaaatt cccccaccag
     2701 tttaaccgac ggctgatttt caaacgcgac gacatccagt tcgctgactg taagttgttg
     2761 ccctttcagc tggccttgaa atttaacttt ttcgccctga taacgcagtt gctggatatc
     2821 agaggttaat gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt
     2881 atcaatattg atccaggtgt taggcagcat ggactgccat tgcgcgaggg tttttggagc
     2941 agctggcgat tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccgt
     3001 gccgacgttc aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag
     3061 ctgacaatct cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg
     3121 tgggctttca tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg
     3181 aacccacagc ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag
     3241 cgcgagaacg gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa
     3301 ttaagcattc aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac
     3361 actaagcata gttgttgatg aatttttcaa tatcgccata gctttcaatt aaatttgaaa
     3421 ttttgtaaaa tatttttagt aacttaaatg tgattcaaca tcactggaga aagtcttatg
     3481 aaactcgccg tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag
     3541 tcctttggct ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact
     3601 gccaatggct gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg
     3661 gaagagctga aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac
     3721 gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat
     3781 ccagaggccg ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac
     3841 cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact
     3901 atgtatggca aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc
     3961 attctgaaag gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg
     4021 ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc
     4081 tctctgcact gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat
     4141 cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag
     4201 gcagcaattg aagcgctgaa aaatcagaaa attggttcgt tgggtatgga cgtgtatgag
     4261 aacgaacgcg atctgttctt tgaagataaa tccaacgacg tgatccagga tgacgtattc
     4321 cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca
     4381 gaagctctga ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggt
     4441 gaaacctgcc cgaacgaact ggtttaatct tgccgctccc ctgcaatcca ggggagctga
     4501 ttcagataat ccccaatgac ctttcattct ctattcttaa aatagtcctg agtcagaaac
     4561 tgtaattgag aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg
     4621 cgggatgtgt aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct
     4681 ttgtgctgga aagcgtaaac ggtaagcccg tgacctgcga taaaaatccg ccagaaatca
     4741 gctttggtga aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca
     4801 aactgtctaa tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta
     4861 acccgcagct taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag
     4921 tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc
     4981 tggcggattt aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac
     5041 agcgtttgcc gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa
     5101 cagaaagcga accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt
     5161 ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac aggctcacta ctacacgccg
     5221 acaacaataa agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc
     5281 ttcaagccaa acgacaccgc cataaataat aggcagcaca gagggcgacg tcgagagctg
     5341 tcctgcgcgt tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat
     5401 caaaacattc attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt
     5461 tgctaaaatt gaaagattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc
     5521 aatgtaaggg tgtcatatga ttactattga cggtaatggc gcggttgctt cggtggcgtt
     5581 tcgcaccagt gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca
     5641 ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg ggagacacac cacgcgtggt
     5701 tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc
     5761 cctttcaaca tcgtttacgt catcgcaggg tttgctgctg atgatcccga cgctgtacaa
     5821 actggcaggc gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca
     5881 tgcactctct atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc
     5941 gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc
     6001 gacgctgaaa agccgcgtgc catttattca tttctttgat ggtttccgta cgtcccacga
     6061 aatcaataaa attgtcccgc tggccgatga cactattctt gatctcatgc cgcaggccga
     6121 aattgatgct catcgcgccc gggcgctcaa cccggaacat cccgtgatcc gcggtacgtc
     6181 agccaatcct gacacttatt tccagtctcg cgaagccacc aacccatggt acaacgcggt
     6241 ctatgaccat gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca
     6301 gccgtttgaa tattacgggc atccgcaagc ggaacgggtg attatcctga tgggctctgc
     6361 cattggcacc tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagttggcgt
     6421 gctgaaagtt cgcctgtacc gtcccttctc cgccaaacac ttgctgcaag ctctgccggg
     6481 atccgtacgc agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc
     6541 gctctatctg gatgtaatga ccgcactggc agaagccttt aataatggcg agcgcgaaac
     6601 tctgccccgt gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg
     6661 tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg
     6721 tatttacgat gatgtgacca atctgtcact gccgttgccg gaaaacaccc tgccaaactc
     6781 ggcgaaactg gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa
     6841 aaacaatatc aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta
     6901 cgactccaaa aaggcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat
     6961 tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat
     7021 cgataaatat cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc
     7081 gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa gttcaggccg tgctaaatca
     7141 gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc gcccgcgaat gtggcctggc
     7201 ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacacaaa ttctgcctgg
     7261 cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggtca
     7321 ggatctggtg gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt
     7381 tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc
     7441 ccctgctttc gtgaaaaccg taaccgctgc gatgctcgcc gggcttggtg atgccctccc
     7501 cgtttcggcg ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg
     7561 caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg
     7621 cgtcgccgct tgcccacact cagctattcg cgcaaaagtg gtaccgcctg aagcgatgga
     7681 aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa
     7741 atatgtcttg caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg
     7801 cccggcgaaa gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga
     7861 acatgtcgaa gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg
     7921 tagcaaactg gaacgtattg atattcgcac atcgcagctg attacaccgc tgtttgaata
     7981 ttccggtgct tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta
     8041 tggcgaccgg atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct
     8101 gccctctaca ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct
     8161 atttgaagat aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt
     8221 ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc
     8281 gttgaaatca gacgccacgc cagaggttcg tcgtgaacag gttgcagctt tacgccagca
     8341 actcaacgat gttgccgaag cacatgaact gctgcgtgac gcagatgcac tggtggaaaa
     8401 atcaatctgg ctgattggtg gcgacggctg ggcttacgat atcggctttg gcggtctgga
     8461 tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc
     8521 caacaccggt ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga
     8581 gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt
     8641 ttatgtggct cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga
     8701 agcggaagcg tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg
     8761 ttacgatctg gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc
     8821 gctatatcgc ttcgatccgc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc
     8881 acgcccgccg tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct
     8941 gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca
     9001 aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta
     9061 aatgctctgg ataaggatta tccaattcta aaaaaaagcc cggacgactg ttcgggcttg
     9121 tctttttata tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt
     9181 gacaataaag
//
LOCUS       DBFIFS010000174         9152 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.174, whole genome shotgun sequence.
ACCESSION   DBFIFS010000174 DBFIFS010000000
VERSION     DBFIFS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.174"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(47..610)
                     /gene="pabA"
                     /locus_tag="N7A07_003644"
     CDS             complement(47..610)
                     /gene="pabA"
                     /locus_tag="N7A07_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU2478234.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(642..1244)
                     /locus_tag="N7A07_003645"
     CDS             complement(642..1244)
                     /locus_tag="N7A07_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU2478235.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(1234..1401)
                     /locus_tag="N7A07_003646"
     CDS             complement(1234..1401)
                     /locus_tag="N7A07_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU2478236.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(1506..2078)
                     /gene="ppiA"
                     /locus_tag="N7A07_003647"
                     /gene_synonym="rotA"
     CDS             complement(1506..2078)
                     /gene="ppiA"
                     /locus_tag="N7A07_003647"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU2478237.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            2349..3530
                     /gene="tsgA"
                     /locus_tag="N7A07_003648"
     CDS             2349..3530
                     /gene="tsgA"
                     /locus_tag="N7A07_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU2478238.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            3792..6335
                     /gene="nirB"
                     /locus_tag="N7A07_003649"
     CDS             3792..6335
                     /gene="nirB"
                     /locus_tag="N7A07_003649"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU2478239.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            6332..6658
                     /gene="nirD"
                     /locus_tag="N7A07_003650"
     CDS             6332..6658
                     /gene="nirD"
                     /locus_tag="N7A07_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU2478240.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            6784..7590
                     /gene="nirC"
                     /locus_tag="N7A07_003651"
     CDS             6784..7590
                     /gene="nirC"
                     /locus_tag="N7A07_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU2478241.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            7609..8982
                     /gene="cysG"
                     /locus_tag="N7A07_003652"
     CDS             7609..8982
                     /gene="cysG"
                     /locus_tag="N7A07_003652"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU2478242.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARHIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         2146 a         2368 c         2398 g         2240 t
ORIGIN      
        1 ataatcacaa aatatgcata aaaaatcatt gaatggcaat cagaaatcag cgatgcagga
       61 aattagccag cagttgatgt ccttgttcgc taagaatact ttctggatgg aactgcacac
      121 cttccagatc ccactggcga tggcgaatcc ccataatctc gcgggtttcg ctccaggccg
      181 tcacttcaaa gcacgctggt aacgagtcag gttccactac cagcgaatga tagcgtgtca
      241 cggtaagtgg atttgccagc ccccgaaata cgccctcacc gttatgtgta atcggcgagg
      301 ttttgccgtg catgaccttt gcggcgcgca caactttacc gccaaatgcc tgcgccattg
      361 cctgatgacc gaggcagacg ccaagaatcg gcaagcgccc ggcatagtgg cgaataacgt
      421 caagggagat cccggcttca tctggcgtac aggggccggg tgagatgaca actttttgtg
      481 gtttaagggc gtcgatatcc gccagcgtca acgcatcgtt gcgcttaacc agcacatccg
      541 cccccagttc acaaaagtac tggtagaggt tccaggtaaa agaatcgtag ttatctataa
      601 gcaggatcat ggcggctccg gtacgaaaga accgcgctat tttactcaga ttccccggct
      661 tcgcttacca ctttgctaaa tatcatctgc aacgcggtga gatcccccat tgccccactc
      721 tgatttgcct gattccaggc ctctttttcg ataccctgcc agctcagttg atatccggca
      781 tgaatcgcca gttgctcgaa gaagatccgc tgtgccagac cacttcccac ccggaaggga
      841 tgcagcacgt tgatttcaca atagtaatgc gccagccgct cgacgaactt cgttttctcc
      901 aggccaacca gataaccttc ttcctccaga tcctgcatca gggcattgcc ctctttttcg
      961 atataagcaa agtggcagaa cggcgtatcg ccctgataaa tatcaacttc acgcagttgc
     1021 cctgcccagt cgaaaatatc ctgatacagc tggcgatgga tagtgcgcaa atgcggtaaa
     1081 ccgcgcacca gcggaccaag ctcaatggtc gcagcacgca gcgccgtcat ttcgtaagcg
     1141 gcctgttcca gccgttgttg ctggcggatg ttcagccggt tacgcatgat atcaaggcca
     1201 ggataaagat acggatcgcg cccttcgccg aatttatcgc tcatagtgac tcctcagctc
     1261 ttcaaggcgc gctaaagcct ctgcggcagt aagagtgact aaaggcatct cgacgccttc
     1321 aagacggcga ctggcctgaa aattacgatt acgctgaagc tcccagagac gggacttttg
     1381 cttatcggtg agtttcttca cttgatgcct ccctgaacgt gtccatttgc cacaagtata
     1441 agcggcaaat ccgggttacg ccgggagagc agaagcgcgg gcaagattgc ccgcgcgaga
     1501 aatcattacg gcaggacttt agcggaaagg ataactaccg gttttgacgg cacattctgg
     1561 tacggaccaa cgtcatgagt cggcacctgg gaaatcttat cggcaacgtc catgcctttc
     1621 accactttac caaataccgc gtaaccgaaa tcacgctgac catggtcaag gaaggcgtta
     1681 tcggcaacgt tgataaagaa ctggctggtg gcgctgtctt tgtcagcggt acgcgccatc
     1741 gcgatggtgc cacgcgtgtt gcgaaggccg ttatcggctt catttttgat tggcgggttt
     1801 ggttttttct gctgcatctg ctcggtgaaa ccgccgccct gaatcataaa gccaggaatg
     1861 acgcggtgaa aggtagtgtt gttataaaaa ccgctgttca cataatcgac aaagttttgc
     1921 acagacactg gcgctttttg tttatccagc tccagttcga tgttaccagc tgaggttgtc
     1981 aacaatacgt gcgggtcccc tttcgctgcc attgctgcgg gagaaagagc agaaagagcg
     2041 aaaacagcag ccatcgccgc cagggtcgat ttgaacatga gatttcctta acaaacagag
     2101 cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa gaacaaggcc atccctttac
     2161 ctaattttac gtatctgaaa tatatgtaac ctgacgatca ctttttattc cgtgatcaaa
     2221 atcacctctt aaaatgcaat ttagcaaccg attgcaataa aacatttaaa cagatcacaa
     2281 aatcacctaa aatcgcccgc tcgcggcata tcccgctatg ccattttatt tttgaacgca
     2341 ggccaatcat gactaacagc aatcgcatca agctcacatg gattagcttt ctctcctacg
     2401 cactgactgg tgcgttggtt attgtcaccg ggatggtgat gggaaatatc gccgattatt
     2461 tcaatctgcc tgtttccagt atgagtaaca ccttcacctt cctcaacgcc ggcattttaa
     2521 tctctatctt cctcaacgcc tggctgatgg aaatcgtccc gttgaaaacg cagttacgtt
     2581 ttggctttct cctgatggtg ctggcggttg ccggtttgat gttcagccac agcctggcgc
     2641 tgttctcggc ggcgatgttc attctcgggg tggtcagcgg catcaccatg tcgattggta
     2701 cattcctggt aacacaaatg tatgaagggc gtcagcgcgg ttcccgcctg ttatttaccg
     2761 actccttctt cagtatggct gggatgattt tcccaatgat cgccgcgttt ctactggcgc
     2821 gcagcattga gtggtactgg gtttatgcct gcatcgggct ggtgtatgtc gctattttta
     2881 ttctgacctt cggctgtgag ttcccggcgc tgggcaaaca tgcaccaaaa acggatgctc
     2941 cggtagcgaa agaaaagtgg gggatcggcg tactgtttct ctctgttgcg gcgctgtgct
     3001 acatcctcgg tcagttaggt tttatctcct gggtgcctga gtatgccaaa ggcctgggca
     3061 tgagcctgaa tgatgctggc acgctggtga gtaacttctg gatgtcatac atggtcggca
     3121 tgtgggcgtt cagctttatt cttcgcttct ttgatttgca acgcattctg accgtactgg
     3181 ctggtctggc tgcgattctg atgtacgtct ttaacaccgg aacaccggca catatggcgt
     3241 ggtcaattct cgccctgggc ttcttctcca gcgcgatcta taccaccatc atcaccctgg
     3301 gttcacagca gaccaaagta ccgtcgccaa aactggttaa ctttgtcctg acctgcggaa
     3361 ccatcggtac tatgttgacc tttgtggtta ccggcccgat tgttgaacat agcggtccgc
     3421 aggcggcact gcttacagca aacggtctgt acgctgtcgt ctttgtgatg tgcttcctgt
     3481 taggtttcgt cagccgtcac cgtcagcata acaccctgac ctctcattaa ttgctcatgc
     3541 cggacggcac tatcgtcgtc cggccttttc ctctctaacc ccgctacgtg catctatttc
     3601 tataaacccg ttcattttgt ctattttttg cacaaacatg aaatgtcaga caattccgtg
     3661 acttaagaaa atttatacaa atcagcaata taccccttaa ggagtatata aaggtgaatt
     3721 tgatttacat caataagcgg ggttgctgaa tcgttaaggt aggcggtaat agaaaagaaa
     3781 tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg taacggtatg gtcggccatc
     3841 gctttatcga agatcttctt gataaatctg atgcggccaa ctttgatatt accgttttct
     3901 gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc gtcttacttc tctcaccaca
     3961 ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga gaaacacggc atcaaagttc
     4021 tggtcggcga acgcgctatc accatcaacc gtcaggagaa ggtgattcac tccagcgccg
     4081 gacgtaccgt tttttatgac aagctgatca tggcgaccgg ttcctacccg tggatcccgc
     4141 caattaaagg ttctgatact caggactgct ttgtctatcg caccattgaa gacctcaacg
     4201 ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt tgttggtggc ggcctgttag
     4261 gtctggaagc cgctggcgcg ctgaaaaact taggtattga aacccacgtt atcgaatttg
     4321 cccctatgct gatggcagaa cagcttgatc agatgggtgg cgagcagctg cgtcgcaaaa
     4381 tcgagagcat gggcgtgcgc gttcacacca gcaaaaacac ccttgagatt gtgcaggaag
     4441 gtgttgaagc gcgtaaaacc atgcgttttg ccgacggcag cgaactggaa gtcgacttta
     4501 tcgtcttctc taccggtatc cgtccgcgcg ataagctggc aacccagtgt ggtctggacg
     4561 ttgctccgcg tgggggtatt gtcattaatg attcctgcca gacttccgat ccggatatct
     4621 acgccatcgg tgaatgcgca agctggaaca accgtgtatt tggtctggta gcaccaggct
     4681 acaaaatggc gcaggtcgcc gttgaccata ttctcggtag cgaaaacgcc tttgaaggtg
     4741 ctgaccttag cgccaagctg aaactgctgg gcgtagacgt aggcggtatt ggtgatgcgc
     4801 acggtcgcac gcctggcgca cgtagctacg tttacctcga cgaaagcaaa gagatctaca
     4861 aacgccttat tgtcagcgaa gacaacaaaa ccctgctcgg tgcggtactg gtgggcgata
     4921 ccagcgacta cggcaacctg ctgcaactgg tgctgaatgc tatcgagctg ccggaaaacc
     4981 cggattctct gattctgccg gcgcactcgg gtagcggtaa gccgtctatc ggtgttgata
     5041 aactgccgga cagcgcgcaa atctgctcct gcttcgacgt caccaaaggt gatctgattg
     5101 ctgccatcaa caaaggctgt cacaccgttg cggcgctgaa agccgaaacc aaagcgggta
     5161 ctggctgcgg tggctgtatc ccgctggtca ctcaggtact gaacgcggaa ctggcgaaac
     5221 agggcatcga agttaacaac aacctgtgcg aacactttgc ttattcgcgt caggaactat
     5281 tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga actgctggcg aaacacggca
     5341 aaggttacgg ttgtgaagtt tgtaaaccga ccgtcggttc gctgctggcc tcctgctgga
     5401 acgaatacat tctgaagccg gaacatactc cgctgcagga ttctaacgac aacttcctcg
     5461 ctaacatcca gaaagacggc acctactcgg tgatcccgcg ctctccgggc ggtgaaatca
     5521 ccccggaagg gctgatggcg gtaggtcgta tcgcgcgtga atttaatctc tacaccaaga
     5581 tcactggctc ccagcgtctg gcgatgtttg gcgcacagaa agacgatctg ccggagatct
     5641 ggcgtcagct gattgaagcc ggcttcgaaa ccggtcatgc ctatgcgaaa gcactgcgta
     5701 tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg cgttggcgac agcgtcggcc
     5761 tcggcgtgga actggaaaac cgctacaaag gcatccgtac gccgcacaaa atgaagttcg
     5821 gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg taaagacgtg ggtattatcg
     5881 ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg cggcatgaaa ccgcgtcatg
     5941 cggatctgct ggcggcggat atcgatcgcg aaacgctgat caaatatctc gaccgcttca
     6001 tgatgttcta catccgtact gccgacaaac tgacgcgtac cgcaccgtgg ttagaaaacc
     6061 tcgaaggcgg catcgattac ctgaaagcgg tgatcattga cgacaaactg gggctgaacg
     6121 cacatctgga agaagagatg gcgcgcctgc gtgaagcggt agtgtgtgag tggactgaaa
     6181 cggtcaatac gccgtctgcg cagactcgct tcaaacactt catcaacagc gacaagcgtg
     6241 acccgaacgt gcagatggtg ccagagcgcg aacagcaccg tccggcaacg ccgtatgaac
     6301 gtatcccagt aactctggtg gaggacaacg catgagccag tggaaagaca tctgcaaaat
     6361 cgatgacatc ctgcctgaaa ccggcgtctg cgcgctgtta ggtgacgagc aggtcgcgat
     6421 tttccgcccg tatcacagcg atcaggtgtt tgcgatcagc aacatcgacc cgttcttcga
     6481 gtccagcgtg ctgtcacgcg gactgattgc ggaacaccag ggcgagctgt gggtcgccag
     6541 cccgctgaaa aaacagcgtt ttcgcttaag cgacggcttg tgcatggaag acgaacagtt
     6601 ttccgtcaaa cattacgaag cgcgagtgaa agacggcgtg gtgcagctgc gcggttaatg
     6661 ttttaacggg aggcgcaatg cctccccttt ttgcatggtc ctgtaataac cttcggtata
     6721 ttgcaggaca ttttttaaac tttttgtttt attttttgtt tttatttttt aaaggataat
     6781 caaatgttta cagacactat taataagtgt gcggctaacg ctgcgcgcat tgcacgcctg
     6841 tcggcaaata acccgctcgg cttttgggtc agctccgcca tggcgggcgc gtatgtgggt
     6901 cttgggatca tcctgatttt tacgctcggt aatttgctcg atccatccgt acgccctctg
     6961 gtgatgggcg cgacctttgg tatcgcctta acgctggtga ttatcgccgg ttctgagctg
     7021 ttcaccggac acaccatgtt cctcaccttt ggggtgaaag cgggcaccat cagccacggg
     7081 caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc tggtcggttc cgtcttcgtt
     7141 gccatgctct atagctgggg tggtggtagc ctgctgccgg tagataccag catcgttcac
     7201 tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac tgttcttcaa aggtgcattg
     7261 tgtaactggc tggtttgtct ggcaatctgg atggcgctgc gcactgaagg ggcggcgaaa
     7321 tttatcgcaa tctggtggtg tctgctggca tttatcgcgt ccggctacga gcactccatc
     7381 gctaacatga cgctgttcgc gctctcctgg ttcggcaacc acagcgaagc ctacacgctg
     7441 gcgggtattg gtcataacct gctgtgggtg acgctgggta atactttatc aggtgccgta
     7501 ttcatgggat tgggttattg gtatgctacg ccgaaagcga atcgtccggt tgcggacaaa
     7561 tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg gtttttacgt ggatcatttg
     7621 cctatatttt gccaattacg cgatcgcgac tgtctgattg tcggcggtgg tgatgtcgcg
     7681 gaacgcaaag caaggttgct gttagacgca ggcgctcgct taacggtgaa tgcattagcg
     7741 tttattccac agttcaccgc atgggcagat gcaggcatgt taaccctcgt cgaagggcca
     7801 tttgatgaaa gccttctcga cacctgctgg ctggcgattg cagcgacgga tgatgacgcg
     7861 cttaaccagc gcgtcagcga agccgctgaa gctcgtcaca tcttctgtaa cgtggtcgat
     7921 gcgccgaaag ctgccagctt cattatgccg tcgattattg accgctcacc gctgatggtg
     7981 gcggtttcct ctggcggtac ttctccggtt ctggcgcgcc tgttgcgcga aaaactcgaa
     8041 tcactgctgc cgctgcatct gggccaggta gcgaaatacg ccgggcagtt acgcggtcgg
     8101 gtaaaacaac agttcgccac gatgggtgaa cgccgccgct tctgggaaaa actgttcgtt
     8161 aacgaccgtc tggcgcagtc gctggcgaac aacgatcaga aagccattac tgaaaccacc
     8221 gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag tggtgctggt tggtgcaggt
     8281 ccgggcgatg ccgggctgct gacactgaaa ggtctgcaac aaattcagca ggcagatgtg
     8341 gtggtctacg accgtctggt ttctgacgat attatgaatc tggtacgccg cgatgcggat
     8401 cgtgttttcg tcggcaaacg cgcgggatac cactgcgtac cgcaggagga gattaaccag
     8461 atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc ggctgaaagg cggcgatccg
     8521 tttatttttg gccgtggtgg cgaagagctg gaaacactgt gcaacgcggg tattccgttc
     8581 tcggtggttc cgggtattac cgcagcttct ggttgctcag cctattcggg tattccgctc
     8641 acgcatcgcg attatgccca gagcgtgcgc ttaattaccg gacacttaaa aaccggtggc
     8701 gagctggact gggaaaacct ggcggcagaa aaacagacgc tggtgttcta tatggggctg
     8761 aatcaggctg cgactattca gcaaaagctg attgaacacg gtatgcctgg cgaaatgccg
     8821 gtagcaattg tcgaaaacgg aacagcagtc acgcagcgcg tgattgacgg tacgctcaca
     8881 caactgggcg aactggcgca gcaaatgaat agtccatcgc taattattat tggtcgggtt
     8941 gttggcctgc gcgataaact gaactggttc tccaaccatt aatttaatca gggccagaga
     9001 attctggcct ttttcgttta caactctctt tatatattca gcagataaaa atataactca
     9061 ttaacaaaaa tcatttgttt ttgccccgtt atattcaccg ttacagcgac ttacaaaatt
     9121 aagtcattca ctcactgacg ccgattaaat at
//
LOCUS       DBFIFS010000175         9109 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.175, whole genome shotgun sequence.
ACCESSION   DBFIFS010000175 DBFIFS010000000
VERSION     DBFIFS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.175"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(130..420)
                     /locus_tag="N7A07_003653"
     CDS             complement(130..420)
                     /locus_tag="N7A07_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU2478243.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            854..1564
                     /locus_tag="N7A07_003654"
     CDS             854..1564
                     /locus_tag="N7A07_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU2478244.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1614..2588)
                     /gene="ghrB"
                     /locus_tag="N7A07_003655"
     CDS             complement(1614..2588)
                     /gene="ghrB"
                     /locus_tag="N7A07_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU2478245.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(2692..3351)
                     /locus_tag="N7A07_003656"
     CDS             complement(2692..3351)
                     /locus_tag="N7A07_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU2478246.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3504..5837
                     /gene="bisC"
                     /locus_tag="N7A07_003657"
     CDS             3504..5837
                     /gene="bisC"
                     /locus_tag="N7A07_003657"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU2478247.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(5806..6246)
                     /locus_tag="N7A07_003658"
     CDS             complement(5806..6246)
                     /locus_tag="N7A07_003658"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2478248.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6243..6806)
                     /gene="tag"
                     /locus_tag="N7A07_003659"
     CDS             complement(6243..6806)
                     /gene="tag"
                     /locus_tag="N7A07_003659"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU2478249.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQTGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            6964..7662
                     /locus_tag="N7A07_003660"
     CDS             6964..7662
                     /locus_tag="N7A07_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU2478250.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            7891..9093
                     /locus_tag="N7A07_003661"
     CDS             7891..9093
                     /locus_tag="N7A07_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2478251.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2136 a         2259 c         2375 g         2339 t
ORIGIN      
        1 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
       61 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
      121 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
      181 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
      241 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa
      301 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
      361 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
      421 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
      481 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
      541 gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
      601 aaccactcct ggtttgtaaa gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct
      661 ggctacaaaa aaggcaacgg caatatcatt ttttaacaat gaaacaacaa attaaccttg
      721 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
      781 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
      841 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
      901 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
      961 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
     1021 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     1081 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa
     1141 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
     1201 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
     1261 ctctgcgcta aaacagagtg atgacctgaa accggtcttt gatcaggcat tcaccaaagt
     1321 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
     1381 acaactggtg atggttggag actacattgc ccaacaaggt actcaggtga gctttgtagc
     1441 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     1501 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     1561 ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc
     1621 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
     1681 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     1741 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg
     1801 aagacatcca gcccggcggc gtgaatttcc cctttctgca atgctgcgat cagtgcattt
     1861 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     1921 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
     1981 aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     2041 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
     2101 cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     2161 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     2221 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     2281 acggtttctg ttaagacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     2341 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
     2401 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
     2461 aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
     2521 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     2581 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     2641 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     2701 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
     2761 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     2821 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcagatcgtg
     2881 accacccgtg ctgtcagtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     2941 caccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg cgctgctgct
     3001 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     3061 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     3121 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
     3181 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
     3241 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
     3301 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
     3361 ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca
     3421 tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat
     3481 ctaacctttc aggagtcttc ggtttgacca actcatcctc acgatattcc gttctgactg
     3541 ccgcccattg ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg
     3601 gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgtgac caggttcaca
     3661 gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc
     3721 cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc
     3781 ttattcacca acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg
     3841 gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct
     3901 atatggcgct ggcaggcggt tataccgggc atctggggga ttattcgacc ggcgcggcac
     3961 aggcgatcat gccgtatgtc gtgggtggta gtgaagttta tcaacagcag accagttggc
     4021 cgctggtgct ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc
     4081 tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttct gcactgcgtg
     4141 acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg
     4201 gcgataaaat ggagtgggtg gcaccgcaca tgggcactga tgttgcgctg atgctgggga
     4261 tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca
     4321 ccggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaccg
     4381 ccgaatgggc agcagagatt tgtggtgtta acgcagagaa aatccgcgag ctggcggcta
     4441 ttttccacca aaataccacc atgctgatgg ctggctgggg aatgcagcgc caacagtttg
     4501 gtgagcaaaa acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac
     4561 ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg
     4621 ctgcggtact ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa
     4681 tccctgtcgc ccgcattgtt gaagcactgg aaaaccccgg tggcgcatat caacacaacg
     4741 gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta
     4801 ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag ctggtggtga
     4861 tctctgaatg cttctggacg gcggcggcaa aacacgcgga tatcgttctg cctgcgacta
     4921 cctcttttga gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc
     4981 cgatgaagca agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg
     5041 agttaagtga acgctgggag aagggcggtt atgcacgttt tacggaagga aaaagtgagc
     5101 tgcaatggct ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg
     5161 aattgccgcc atttactgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc
     5221 cggacagcga gcggtttatt cgcttcgctg atttttgccg cgatccgcag gcgcatccat
     5281 taaaaaccgc cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc
     5341 cggattgccc ggggcatcca atgtggctgg tgccggacga atggcagggc aatgccgaac
     5401 cggaacagtt acaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt
     5461 acagttctct gcgcgaattg tacgcggtgg caaaccgtga gcctgtcacc attcatcctg
     5521 acgatgccca ggcgcgcggc atacaagatg gcgatactgt tcggctgtgg aacgcacgcg
     5581 ggcaaattct tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc
     5641 acgaaggggc atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag
     5701 tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata
     5761 cggcgctggc atggctagaa aaatacaacg gtccggaact gacacttaca gcgtttgaac
     5821 caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac
     5881 aatgtgaaaa ccctgcgcct ggtaaaaatc tatcgccggt tgattttttt gataaacctc
     5941 cagcatcaga tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg
     6001 tctgacggcc ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac
     6061 aaaaccgaga agcttaccgt cttcttccca gacccagttt ttcgcattgg caagatacgc
     6121 atcccgcacc agcggaatgc actcgcgcca gtaattcact tttataaagg gatgccccca
     6181 ggttgtactt tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg
     6241 aatcatggtt tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca
     6301 catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttc
     6361 agtgccttag atagggcgtc ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg
     6421 gcttgtgtca cctgtggctg atgatttaca aacgaccaga caaagtcggc aaacggttcg
     6481 ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg
     6541 cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgcg
     6601 gcaaccttca ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg
     6661 acggtgatcc acgataaccc agtctgctgc ccttccaggc agatcatttc gaacagtttt
     6721 ttcctgtcag tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc
     6781 tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt
     6841 tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc
     6901 ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg
     6961 ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta
     7021 atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg
     7081 caacccgggc attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac
     7141 gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg
     7201 gcggaagaaa cccctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta
     7261 tatgaacagg ttacaactgg cccggtcgcg gcattacatt acgatggcac aaccacctcg
     7321 atgtataacg agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc
     7381 gtgagtacct taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa
     7441 atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg
     7501 atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg
     7561 ctcaatcaaa atattgccgc ctatgccgcg ctatctcagg cagaaaatac cactaacaat
     7621 agcgattatc tgtatacgat gggggttagt gccagatttt aacgtaacaa tcacaattga
     7681 aaccattgaa taacaatagt tgtggcgata gtgggtgcta acttaccaaa taataaattt
     7741 ggtgaataat tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc
     7801 atttcatgcc gtttttcccc aggcataaag tgcacttcgt tatggttgtc ggcagagatt
     7861 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc
     7921 tggttgacac tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg
     7981 agcctgttta acggcgcgct ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc
     8041 tctttcggct tgttaagtct ggggctggca atatcgtctt ctgttgcggg caaattgcag
     8101 gaacgttttg gtgttaaacg cgtcaccatc gcttccggca ttttgctggg attagggttc
     8161 ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc
     8221 ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa atggttcccg
     8281 gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt
     8341 ttcaaattta tcgacacgca gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt
     8401 tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca
     8461 aaacaggaag tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg
     8521 atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc
     8581 ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt
     8641 tctgcagcca atgcagtcac tgttatttcc atcgccaacc tttcaggtcg tctggtgctg
     8701 ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtaatttcg
     8761 ctggtgggta tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg
     8821 attgcctgcg tggcatttaa ctttggcggc actattaccg tctttccgtc actggtcagt
     8881 gagttcttcg gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt
     8941 atcggtagca tttgtggttc gattatcgcc tcactgtttg gcggcttcta tgtgaagttc
     9001 tacgtcatct ttgctctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca
     9061 gagcagaaag tattgcgtga ggcgcatatt tagccagaaa ctacaggtt
//
LOCUS       DBFIFS010000176         8831 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.176, whole genome shotgun sequence.
ACCESSION   DBFIFS010000176 DBFIFS010000000
VERSION     DBFIFS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.176"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            2..181
                     /locus_tag="N7A07_003662"
     CDS             2..181
                     /locus_tag="N7A07_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2478252.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            222..494
                     /locus_tag="N7A07_003663"
     CDS             222..494
                     /locus_tag="N7A07_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2478253.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            571..786
                     /locus_tag="N7A07_003664"
     CDS             571..786
                     /locus_tag="N7A07_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2478254.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            791..1324
                     /locus_tag="N7A07_003665"
     CDS             791..1324
                     /locus_tag="N7A07_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2478255.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALSCWGIDR"
     gene            1598..2167
                     /locus_tag="N7A07_003666"
     CDS             1598..2167
                     /locus_tag="N7A07_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU2478256.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2167..2316
                     /locus_tag="N7A07_003667"
     CDS             2167..2316
                     /locus_tag="N7A07_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478257.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            2324..2788
                     /locus_tag="N7A07_003668"
     CDS             2324..2788
                     /locus_tag="N7A07_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2478258.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGYRSLRINATCPGP
                     VREATGTARVDNATGPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(2820..3113)
                     /gene="bor"
                     /locus_tag="N7A07_003669"
     CDS             complement(2820..3113)
                     /gene="bor"
                     /locus_tag="N7A07_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU2478259.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGTENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            3522..4328
                     /locus_tag="N7A07_003670"
     CDS             3522..4328
                     /locus_tag="N7A07_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2478260.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTNQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            4309..6015
                     /locus_tag="N7A07_003671"
     CDS             4309..6015
                     /locus_tag="N7A07_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2478261.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            6015..8159
                     /locus_tag="N7A07_003672"
     CDS             6015..8159
                     /locus_tag="N7A07_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU2478262.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     DTEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQL
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            8317..>8831
                     /locus_tag="N7A07_003673"
     CDS             8317..>8831
                     /locus_tag="N7A07_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478263.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYL"
BASE COUNT         2364 a         2012 c         2430 g         2025 t
ORIGIN      
        1 tatgacattt ctgaaccagt taatgctgta cttctgtacg gtggtctgtg tgctgtatct
       61 cctttcgggt gggtacaggg ccatgcgtga cttctggcgc agacagattg acaaaagggc
      121 cgctgagaaa atcagcgcca gtcagtcagc cggaagcaaa cccgaagagc cgctcattta
      181 gcggcaactt tcttaatcac acctttcgac gagaaaatcc catgtcagaa attacatccc
      241 tggtcactgc agaggcagtg aaggacgtcc tgcgctctga agaagtccgg agcgcactga
      301 aacagaaact tcgccataac ctggaagcgc gtcttgatgc agaagtggat gccattctgg
      361 atgaactgct tggtgtacag gcagagccac cgactgaagc gggagatacc accgcagaga
      421 gcggtgaagt tcagcctgaa tcaccggtcg ccgatgcgac tgaacctcaa cccgaatcgg
      481 tcatgatgct gtaacgggga gtcagggcca tcagtaaaca gctgctggcc tttttcatgt
      541 tgtgagcttc cggattgcgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt
      601 gtgtcataca ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg
      661 gtttccccgt ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tggtctgctg
      721 acgtacctga cgaacctgta tttcaagatt aaagaagacc ggcgtaaggc ggcgcgggga
      781 gagtaagctg atgagcagga aattccgcta tggtttatca gcggtagttc tggcgctgat
      841 tgccgcaggt gcttctgcgc ctgaaatcct cgaccagttt ctggatgaaa aggaaggtaa
      901 ccacaccacg gcataccgtg atggtgtggg tatctggacc atctgccgtg gagccacccg
      961 ggtggatggt aagcctgtga ttcctggcat gaagctgtcg aaggaaaaat gcgaccgggt
     1021 taacgctatc gaacgggata aggcgctggc atgggtggcg aagaacatca gagtgccact
     1081 gactgaaccc cagaaagcgg gtattgcgtc attctgtcct tacaacattg gccccggtaa
     1141 gtgtttcccg tcgacgtttt ataaacgaat taatgcaggt gatcgaaaag gtgcctgcga
     1201 agcgattcgc tggtggatta aggacggtgg cagagactgc cgtatccgct caaataattg
     1261 ctacggtcag gtatcaagac gtgaccagga gagtgcgctg tcatgctggg gtatcgacag
     1321 ataagcagaa tattttgctg aaaaatgacg ttggccaacg cggcagataa cacgaaatcc
     1381 tgcaaactgg caaaatgtaa gtgaataaag ttaacaagat tgtttcatga agaggcaccg
     1441 taatggtgcc tttgtcattt ctgcgcttcg cacaagcgta aataaaccaa agaacctttc
     1501 aggatgagcc ctggtggata accggcagtg gtctggttaa ccctctttgg gctggttatt
     1561 cctgtgcgca gggttcatca ctaaaaggaa ataaccgatg aatatgatga ccgtgccgtt
     1621 tcacggcgat tctctttatg tggttaacca taacggcgaa ccatacgttc ccatgaaacc
     1681 tgtcgttgcg gggatggggc tggcctggca atcacagttg gctaagttaa gacagcgttt
     1741 tgcgtcaact ataacggaaa tcgttatggt tgctgaggat gggaaacgac gcaatatggt
     1801 gtccctgccg cttcgaaaac ttgcaggctg gttacaaacc atcaatccca acaaagtaaa
     1861 acccgaaatc cgcggcaagg taatccagta tcaggaagag tgtgacgatg ttctctatga
     1921 atactggacg aagggttttg tcgttaatcc ccgtcgaatg agtgtgatgg aagaactcaa
     1981 tcaggcttgc gctgacatga aacgggataa aaacattgcc agtgtgtttg ctaccgggct
     2041 gaatgagtgg aaacaggtta aatccgcgca tgtatcaaaa atccgcacat tgataaacga
     2101 agcgaatctg ctgattgatt ttgtcctggc tgatacaggc aaagggaaaa taacaaaggc
     2161 ggattgatgg agtggtggct aatgatatcg gataaactca taacgctggc gaagatcctc
     2221 tgtgtaatcg tcggcatttc atttttagtc atgctggttg ccattttctt ttccaccgcc
     2281 tggcgagtcc tgacgttatc gggactggtg gggtgaaaga gagatgaacc gtgttctgtg
     2341 tgtggtgatt attgtcctgc tggtagcctg tggtgcgctt agtctggggc tgaatcatta
     2401 ccgcgataac gccataacct acaaagagca gcgcgataaa aaagtcagtg agctggagct
     2461 ggcaaatgca accattactg atatgcagca gcgccagcgt gatgttgctg cacttgatgc
     2521 cagatactcg agggaattag ccgatgcgag agctgaaaat gaaactcttc gcgctgacgt
     2581 tgccgctggt taccgcagcc tgcggatcaa cgccacctgt ccaggtcccg tgcgtgaagc
     2641 caccggcacc gcccgcgtgg ataatgcaac cggcccccga ctggcagaca ccgctgaacg
     2701 ggattatttc accctcagag agaggctgat cactatgcaa aaacaactgg aaggaaccca
     2761 gaagtatatt aatgagcagt gcagatagag ttgcccatat cgatgggcaa ctcatgcaat
     2821 tattgtgagc aatacacccg cgcttccagc ggagtataaa tgcctaaagt aataaaaccg
     2881 agcaatccat ttacgaatgt ttgctgggtt tctgttttaa caacattttc tgtgccgcca
     2941 caaattttgg ctgcatcgac agttttcttc tgcccaattc cagaaacgaa gaaatgatgg
     3001 gtgatggttt cctttggtgc tactgctgtc tgtttgtttt gaacagtaaa cgtctgttga
     3061 gcacatcctg taataagcag ggccagcgca gtagcgagta gcattttttt catggtgtta
     3121 ttcccgatgc tttttgaagt tcgcagaatc gtatgtgtag aaaattaaac aaaccctaaa
     3181 caatgagttg aaatttcata ttgttaatat ttattaatgt atgtcaggtg cgatgaatcg
     3241 tcattgtatt cccggattaa ctatgtccac agccctgacg gggaacttct ctgcgggagt
     3301 gtgcggggat aatcaaaaac gatgcacacc gggttttctc atttttcacg agatgggagc
     3361 gatttcccgc gaagccgcct gtccggtgcg gtggtggaag aaaccggata aaacaaccgc
     3421 attgtgcaaa tatcgatcaa atatggtgct gctgtgtgaa atctgaaaaa tcacagcggt
     3481 cattatgcat cagtttttaa cacaggacgt cagaacgtga catggcaaag ctggactgga
     3541 aaaagctgga gcaggcattc cgacgcgaac atgccgaaac gggaataaca ttactggact
     3601 ggtgccggaa gaaaaagatt aattacaaca ccgccagaac ccgtataaaa atgggcaaaa
     3661 tcgatcatga aattgatcat aaaaccgatc atgaaatcga tcatgacatc tcagatgaag
     3721 aaccctgcaa tgacgcgggt tccggcgatg aaaaatgcgc aaaaaactct gaaaaaaact
     3781 gcgcaaattc ggcagaaacg aaacggattc gtggttcccg acttttacca ccttcaaacg
     3841 ctttttctca gcgaaacacc cacgccgtaa gacaccgtgg atatgcgaag tatcttgagg
     3901 cagataacct catggatgat gcgtccgaca tggtgctgtt cgatgaactg gtgttcaccc
     3961 gggcccgcgc actttcagta actaaggcac ttaaagggat gttcgccgac ctggaagagg
     4021 caactgacgt ggaaacccgt gttgctcttt acgacaaaat actcaaagct gaacaggccc
     4081 ttgaccggaa tattgcccgt atcgagtcaa ttgaacgctc attgctgacg ctggacgtcc
     4141 tggctgagac agcaccaaaa cttcgtgctg accgggaaag aatcaacgcc gccagagata
     4201 aactcagagc tgaaaccgat attctgacca accagcgtcg gggcgttgtt acgcctgtca
     4261 gtgacatcgt gtcatcgctg catgaaatga gtaattcggg gagactggat gacattccgg
     4321 aagaatgaac cgcgatgtga tgagccgtca gaaatgaccg aggctgaaca acgtctgttc
     4381 atcatgacta aactgagcaa tccctggtgg cggctcaatc atctctacaa aatacagaac
     4441 gaaaaaggtg aactggtcac cttcagaatg cgaccggcgc agcgccagtt gttccggagc
     4501 atgcacaata aaaatattat cctgaaagcg cgccagctgg gattttccac agccattgat
     4561 atttatcttc tcgaccaggc attattcatt ccgcatctca aatgcgggat cgtcgctcag
     4621 gataaacagg ctgccagtga aattttccgc acaaaaattg ctgtaccgtt tgatcatctc
     4681 cctgactggc tgagagcctc attcaccatc gttgaacgtc gtagcggtgc cagcggtggc
     4741 tatatcctgt ttggtcacgg ctcgagtatc caggtggcaa cctcattccg ttcaggtacg
     4801 gtgcagcgcc tgcatatctc agagcacggc aaaatttgcg cgaaatatcc ggctaaggcg
     4861 aaagaactgc gaaccggtac gcttaatgcc gtctctgatg aatgcattat ttttgatgag
     4921 tccactgctg aaggcgtggg tggtgatttt tacgagatga gtaaccgagc acaggagatc
     4981 actgcatcag gcttattgct gacggcacag gattataaat tccattttta cgcctggtgg
     5041 caggatccta aatacagcgc cagagtgccg gaaagcgggc tgaagctgtc acgggaaaaa
     5101 atgacgtatt tttctgcggt tgagaaggca atgaacatca cgcttactga tgaacagaag
     5161 cagtggtaca tcaataagga aactgaacag cgtgaggaaa tgaagcagga gtttccctca
     5221 acgccacagg aggcgtttct gacgtccgga cgacgtgtgt tcagtgccga aagtacgttg
     5281 caggcagaat cattctgttc gccaccgatg attgtttatg acattgaacc tgttacagga
     5341 gcgaagacta aagctcagtc tctgcgtgaa ggaaataaaa acgagttgca gcggacgctg
     5401 atgaattatc tgctggtatg ggaactgccg gatccggatg aagagtatgt ttgtggggca
     5461 gatactgccg aagggctgga gcacggagac cgctcatcgc tggatgttgt caaacgcagt
     5521 aatggcgagc aggtggctca ctggttcggg catctcgatg ctgaactttt tgctcatctc
     5581 atttcgcagg tctgtcgtat gtataacaac gcgtttgtgg ggccggagcg taataatcac
     5641 ggacatgcag ttatcctgaa actccgggaa ctctatccga cacgttatat ctacaacgaa
     5701 cagcatcttg accaggcata tgacgacgat acgccccgcc ttggctggct gacaacccgt
     5761 cagagcaaac ctgttctgac cgaaggaatg aaaacgcttc tgaataatgg aatatcaggg
     5821 atccgctggt caggcacatt atcggaaatg aacacctacg tttatgacgc gaaaggctcc
     5881 atgaatgcac aggaaggctg ctttgatgat cagctcatga gctacatgat tgcccaggag
     5941 atgcgcgcca gaatgccggt gagggtaaaa cagaaaacgg ataaacgcag aaccacacac
     6001 tggatggcac actgatgaaa aatgaaatta acaccacagc gatgaaaaac gatcatggat
     6061 ccacgccgcg tttttctcag cgtcagttac tgtctctctg ttctgatatt gacagtcagc
     6121 ctctctggcg tgatgccgca aacaaggcct gtgcgtatta tgatggcgac cagctggcac
     6181 cggaagttat ccaggtactg aaagatcgcg gtcagcccat gaccatccat aacctcattg
     6241 cccccacggt agatggtgta ctgggaatgg aggcaaaaac aagaacggac ctgatagtga
     6301 tgtcagacga tccgaacgat gatacagaga aactggcaga ggccattaat gcggagtttg
     6361 ctgatgcgtg ccgtcttggc aacatgaata aggcccgctc cgatgcctat gcggaacaaa
     6421 tcaaggcggg cctcagttgg gtggaggtca gacgaaacag tgatccgttc gggcctgaat
     6481 ttaaggtgtc tactgtcagc cggaatgagg ttttctggga ctggctgagc cgggaggctg
     6541 atttaagtga ctgccgctgg ctgatgcgtc gccgctggat ggataccgat gaggcaaaag
     6601 ctacattccc gggaatggct caggttatcg attatgccat tgatgactgg cgtggttttg
     6661 tcgataccac ggttactgaa ggccagccca gtccgttgat gagtgcatgg gaagagtatc
     6721 agtcatggga tcgacagcag aacgaatggc ttcagcgtga acgccgtcgt gtgctgcttc
     6781 aggtggttta ttaccgtaca ttcgagcgtc ttccggtgat tgaactcagt aatggacggg
     6841 tggtggcctt tgataaaaat aatctgatgc aggcggtagc tgtggcatcc gggcgggttc
     6901 aggtgaaagt cgggcgggta agccgtattc gtgaagcctg gtttgtcggg ccgcacttta
     6961 ttgtggatcg cccctgtagt gctccgcagg ggatgtttcc gctggttcct ttctggggat
     7021 accgaaagga taaaaccggg gagccatacg ggctaatttc ccgcgccatt ccggcacagg
     7081 atgaggtgaa ttttcgtcgt atcaagctga cctggttgct tcaggccaaa cgtgtgatta
     7141 tggacgagga tgccacccag ttgtcagaca acgacctgat ggagcagctc gaacgtccgg
     7201 atggcattat taaactgaat ccggtccgaa aaaatcagaa aagtgtcgca gatgtttttc
     7261 gggttgagca ggattttcag gttgccagcc agcagtttca ggtcatgcag gaatcggaaa
     7321 aacttatcca ggataccatg ggagtgtatt ccgcatttct cgggcaggat tcaggtgcga
     7381 cgtcaggcgt ggctatcagt aacctggtgg agcagggggc cacaaccctt gcggaaatca
     7441 acgataacta ccagtttgcc tgccagcagg tgggaagact gttgctggct tatcttctcg
     7501 atgacctgaa aaaacgccgt aatcatgcag tggtgattaa tcgcgatgat cgccagcgtc
     7561 gccagaccat tgtcctcaat gctgaaggtg ataatggtga actgaccaat gatatttcaa
     7621 ggttaaatac acatattgcg ctggcgcctg ttcagcagac accggcgttt aaggcacagc
     7681 ttgcacagag aatgtcagag gttattcagg ggctgccgcc tcaggtgcag gctgttgtgc
     7741 tcgacctgtg ggttaatctt ctggatgtgc cgcagaaaca ggagtttgtt gagcgtattc
     7801 gtgctgcgct ggggacgcca aaatcaccgg atgaaatgac gccggaagaa caggaagtag
     7861 cggcacaaca acaggcactt cagcaacaac aggcagaact ccagatgcgc gagatggctg
     7921 gcagagtggc aaaactggaa gctgacgccg ccagggcaca tgcagctgca caacgggata
     7981 atgccagtgc acagcgggaa gtcgccctga cacaggggca gcgttatgtg gatgcgctta
     8041 accaggcaca tacggcagaa atcattaccg gcgtacagaa tatggaacag gagcaggacg
     8101 ttcttcagca acagatgctg tatacgttac aacagcggat gaatgaaatg tcgctctgaa
     8161 aactctggct tcaactgaac cccgtcatcg tacggggttt tttgtttccg gaggtaagcg
     8221 ttccgggagc ggtgcgctta ttcgcggggg cagcgataag ccttatttac tcaaccattc
     8281 ggatctgtcc gataaacaga ccatgcggag ttatttatgg attttgaatt tacgggtgaa
     8341 gaaaccccgg aacaactgga aaaaatgctg gaaggacttg gggatgtgga tattgacagt
     8401 cacgcacagg acgtcgtgac ggaagatacc acggaaaaac atgcggatga ggaagcacag
     8461 actcagacgg gcgataacaa tgtggcaccg acgccggatg ccagtgtgga gcagacgcag
     8521 gacgtgaagg agccggaagc gaagggggtg ctcacccgcg acggtaaaca cgtcattccc
     8581 tatgaagtcc ttgaggctga acgttccggt aagcaacggg ccgaacagga agccgcactt
     8641 cttcgtgggc agatagctga agaaaaacgc agggtggaac tgctgacgtc tcagatccac
     8701 caggccggta tgaagcccac accgttaccg gaaaacgaaa aaatttctga tgagcagatt
     8761 gcccgtatca gggagatgta tccggaaatt ggtgacgcgg tggcttcgct catccgtaaa
     8821 aataactatc t
//
LOCUS       DBFIFS010000177         8750 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.177, whole genome shotgun sequence.
ACCESSION   DBFIFS010000177 DBFIFS010000000
VERSION     DBFIFS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.177"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(110..2499)
                     /gene="dosP"
                     /locus_tag="N7A07_003674"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             complement(110..2499)
                     /gene="dosP"
                     /locus_tag="N7A07_003674"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            complement(2524..3906)
                     /gene="dosC"
                     /locus_tag="N7A07_003675"
     CDS             complement(2524..3906)
                     /gene="dosC"
                     /locus_tag="N7A07_003675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU2478264.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(4283..5602)
                     /locus_tag="N7A07_003676"
     CDS             complement(4283..5602)
                     /locus_tag="N7A07_003676"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU2478265.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5733..7268)
                     /gene="gadC"
                     /locus_tag="N7A07_003677"
     CDS             complement(5733..7268)
                     /gene="gadC"
                     /locus_tag="N7A07_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU2478266.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(7424..>8750)
                     /locus_tag="N7A07_003678"
     CDS             complement(7424..>8750)
                     /locus_tag="N7A07_003678"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU2478267.1"
                     /translation="IAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDD
                     ENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSS
                     EACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPG
                     QLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHID
                     AASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVF
                     NVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAK
                     LGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATD
                     IVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         2229 a         2172 c         2136 g         2213 t
ORIGIN      
        1 agaggggaag ttaaggaagg aatctcccgg aatcgtagct gaaatcacag tatttaagtg
       61 acagtgtcac gttaaatgaa aacccgcgag tgcgggagag aggaatttgt cagattttca
      121 gcggtaacac gctgctcatc cagcctggaa tttcttcggc gggtaggggg cgggaaaaga
      181 aatatccctg aataacgcga cagtggatct ttcgtagcat ctcaaattgc tctttggttt
      241 cgacgccttc cgccacgacg gttaaattga ggctttgccc aatgctggta atggcttcaa
      301 gtaaggccag gatgcgtttt tccgtcagac aacgatcgac aaaacttttg tcaattttga
      361 tttccgttac cggaagactg actaagcggg ataatccgga aaagcccgtg ccaaaatcat
      421 ctaccgataa acccacgccc atatcacgca ggatctgaat gcgcttaaag atttcggtat
      481 cgtgttccat catcatgctt tccgtgattt ctaccgtcag ctggtggccg tcaatacccc
      541 aggcttgcat tgcatcagac acctgattag gcagctgatt actgcgaaag tgcagcgccg
      601 acaagttcac ggataacgcc gggatatgaa tattctggct acgccattct gctaactgac
      661 ggcaagcttc cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc tcttctgcga
      721 gaggaataaa ccgtgaaggg ggcacatgac catgctgggg atcgtgccag cgagcaaggg
      781 cttcgatgcc gtagagttca cccgtttctg cgaagatttg cggctggtaa accagtttca
      841 gttggttatt gctaatcgct tctttcagcg ctgcgcctaa aaccaaacgc tcttttacca
      901 tttcgttcat cgccgggctg aagaactgcc agccgttacc gccattcttg cgaatataat
      961 ccatcgcgtt atgggcagtg gagagcaggt aatcgcgatt tttacccacg tcgtagctga
     1021 tgccaatact caaggtaagc gggaaggggt tatcgtcaat cattatcggc ttgctgacca
     1081 cattccgtag ctcatcggcg atctgggtaa tgttactgac gtcgttttcc aggctcacga
     1141 ggacaaactg cgtaccttcg atacgacaga gatactgatc cggtttgagt ttttcacgaa
     1201 agcgattgac cacttccagc aatgcctgat cggcccacgc atagccaagg ctatcaatca
     1261 catcctgaat atggtcaaca ccgatactgg agagacggct ttgtcgacca ggtcatcgag
     1321 gtaattgtgc aggttattgc gatttggcag accggtcatc ggatcaaatt ggatgagttg
     1381 ttcaatatgc tgacggcttt tttcctgttc cagcgccagc gcggccatat gctggctgat
     1441 atctgccacg cgttcgataa aggcgctggt ttctgctcct gacgaggttt taatttgcag
     1501 gatccccgca ggcgcgccat cacgctgacg aatggtcgct gaccagcttt gcgcattttg
     1561 aacttctgca ccgtgcgaag atgacgccca gtgtatcggc atcccgttgc gcagtgcgaa
     1621 cagcgaaaca tgcgattcgt tgagaacaga ttcgatgtta cgacaaatga tttcccccat
     1681 ttcatgaaat ggcgggctgc tgcacatggc ggcgagaata ttgccttcaa gctggcggat
     1741 ttgccgttct tcggtgatat ccgagaaggt cattaccagg ttctgcagat gcgcgagcac
     1801 gtcataaacc gggctgatag aagctttaat ccagattttt tcaccggtgc gcgtcaacag
     1861 cagaaattcg tcctgatcgc gggcggtttt ccaaagcaac tgttgtaaac gaatgcggtt
     1921 atcggcaggg aattcaggaa tgttcaggag tgtatcaggc tgcataccgc tggcttcgct
     1981 aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac tgcacaatat ggcgttccgg
     2041 atcgaggaca atcaccggtc ggtcgagatg gtcaacggca ataatcaatt gcctggtctg
     2101 ttctttttgc gccatttcta cgctggcatc ccgtaccagc gccaggtaat aaactttccc
     2161 ctcggcgctc actttcgata gcgcaaaacg ggtccagatt ttactgccgt cttttttctc
     2221 cagctgcagc tcccgactca tcccctcgac gcgcgcttta ccgccttcac ggttgtgacg
     2281 aatgtattca ggatgcgcag gacgcaaatc ccgcggaatc agcatatcaa tgttattgcc
     2341 aatgacttct tcacgtttgt acccccagag cttctctgcg gcggggttga aaaacatcac
     2401 ttcatcattt tcgttaatta acaccgcacc catcatattt tgctcaaggg cggggaaaaa
     2461 aatgccatcg gcggcattat ccgcatcggt tagcttcatg attacctctg catcctggcg
     2521 catctaaaga ctggctttcc agagttcaac acggtttcta cctcgtcttt tggcgatata
     2581 cagagcttca tcggctattt gaatgaggcg ctcatagtca ggatgaccat taaacatggc
     2641 ggcaccgatg gaaagtgaga gggcaatatc ttcgccgttt gcggctttca gtttggtttt
     2701 ctccacccga ctgcgaatac gttctgcggt acgtaacgtt tcgttttcag aagcttcagt
     2761 caaaacaatg ataaattcat cgcccccgta gcggaaaaca taatcactac tgcggacgtt
     2821 gtcataaaag gcctgagaga ctttacgcag aatttcatcg ccagtgttat ggccccacgt
     2881 atcgttgatc tctttgaatt tatcaacgtc aataatcagc actgacagcg gtgtaccggt
     2941 ccggttggca tgggcaattt cgcgtttgaa gatagtcggc aggaaacggc ggttaagtaa
     3001 tttcgtcagt acatccatac cgacttcgtg gcgcgatact tcttcaaaca attcacgcag
     3061 caaggtaata atttgcgata cggtatttct tatctgtaat aaaaatttca cccgcagact
     3121 tctgttattc aaattcctgg tgttacgcat ggtttgattg aaaataccgt cgaaatcctg
     3181 aatcagacga gagatatggc ccacttcagc aatgccgctg aaataatgtc gacctttatg
     3241 gttaaaccac aggccaaaat cagcctggct taaaggcaaa ctactgccta aatcagaatc
     3301 cagcaggatt ttatagataa tatctatttc ccatgaaagt attgaggcta tttgccgttc
     3361 tttttcttct tcggcgtttt ccagtaacga gaagatacga tagttttcat cttcttttga
     3421 ggcactactg tcactaaagg taaacgcgcg ggtcatcact tccatagcga tatcaatact
     3481 gttaatcgag aaatggtaga cctgaagttt ttctgcggca gaataatccg aagagaagat
     3541 taccggatag aggatctttt tcagcacccg aaaccccatc tcgacaattt ctaccggaat
     3601 tcctatgcgg gcatgcactt ccgcgacggt atgctggatt tgtattagcc tttcgacatc
     3661 gtcaacctgg gcagaaagca cgttaataat ccagcgttcc atcgcacttt tcaactgccg
     3721 ctcaacttgt tcattactca agaattcttc ggcatgcggg tcgatgcgga caattcgata
     3781 aaactcgata ctcagataat gagcatgcgc aacggcaatt tccgcggctt tagcacgaat
     3841 gggcggatct gcctgctcga caagtccggt ccactcatct ttcattcttt taaaatacat
     3901 ctccataatt cacaccctta taaggctggg aaatcagacg gaatcaaaat gaaacgcgac
     3961 gtgcgagatc gactaactgc accatattct cctgaaatat gaagatatac tgaaaagaaa
     4021 taagcgactt aggacagttt caatctacgc tactgttctt cagaagagta tagcccatcg
     4081 taattatttt tcggtgactg cgaatatcgt atggtttttc atattcatac atttttatta
     4141 ggggatttat ggctgtttaa ctaagcgtgg ttaattgcgt ggttaatttg actttagtaa
     4201 gcatgattgc tggtgggata gttcaagagg gtaacaagcc agtgggtaaa gcaccggctt
     4261 gttacaaagt aagaatggga gtttaactgc cccaacgact ttgcagatag ctgaccgctt
     4321 gttgagtctg cggtttattc agatagtcct cacggaacaa gatggtgccg ctaatttctg
     4381 gtaccgcatc gttaagatcg agctgctttt tcagctccgg tacgccgccg ttaatcatcc
     4441 agtctggctc tatctttgaa ggttcaccca ctttatagaa ggcgataccg atatacaggc
     4501 gggtcctggt cggtttaacg acatccgccc accattttgc caacacgtca taacgcgcgg
     4561 cactccgtga gaacggccag taaatttggg gagcaatgta atccagcaat ccttgttcca
     4621 cccatcgacg agtgtcagcg taggattcgt cataggctgc cgcgcctcgg gtatcggaac
     4681 cgagcggatc gtgtgatcgg ttacgccaca cgcctgccgg gctaacacca aattcgactc
     4741 ccggcttaat gcttttaatg gtgtgcgata cctttgcaat taactgctga gtattgttgc
     4801 gccgccagtc tgcttttgac gcaaatgcgc ctccgtattt acggtacgtt tcgttatcat
     4861 ttagccgtga acccggtgac tcagtataga aatagtcgtc aaactgcacg ccatctaccg
     4921 gatagcggga aaccacttct gcgactattg atgtgatcca gtcctgaacc tcagggatgc
     4981 ccgggtcgag gacaaagcga tcgccagacg ttctgatcca gtcacggtgt tgcacataga
     5041 cgctcgccgg ttgttgagac agagtgctat tcagttccct gatagtaccg ggcttcgtat
     5101 taaccgatac gcgatagggg ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt
     5161 cgtcgagcat aaattgcagc ggatcgtaac ccggattttc accaatctta ccggtcataa
     5221 gatcggacca cggcaaaatt ttcgatggcc acagggcggt accgtccggc ttgacctgga
     5281 aaaagaccgt gtttataccg agacgttgca aatgatccag tttgtcgatc atcgcctgtt
     5341 gttgtacacg ggcccggctg gtggggttac taatgttaac cgaggaaacc ggtggccagt
     5401 cgaggcgaga caccgtggcc agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg
     5461 tggctggtgg ctttgaaccc gctggtggtg tcaccatgga ctctggaggc gtgcttttac
     5521 aactacacag taaaagtgcc agtgcaacca gtatcgctgg tcttctaatc gttaatttct
     5581 tgtttcggga gcagatatcc atatgtgcta gtttccggta aacagatgtg cgctcattct
     5641 cgtacttatc ccggttaagt caatacgaca gcaagcacga aaaagggagc gatgaattat
     5701 cgctccctta tcttataacc attcagacat agttagtgtt tcttgtcatt catcacaata
     5761 tagtgtggtg aacgtgcacg cgggtgcagg aagaagtgac cttttggtgc gttctgactg
     5821 ttgattggct ccagagtgac gccggtattc gctttgcctt tacgatcatg aacagcatag
     5881 agaataaagg gcagggcaag taccaccagg aaactaacga ccagtaattc aacatacata
     5941 tcggtagagt caccctggat gttatccggc ggcaggaagg aaacaataaa cgccataatt
     6001 gaagtcagca gaccgacaat tgccacgacc agtttcaccc ctttaccacc agggatatta
     6061 aatgtgcgtt ttaagtcagg atgtttaaga accaacacaa tgtagccaat aaacagcatg
     6121 aaataagcac acagataaat caccaccgtc agcgccagtg cgatcaggaa ggacatgttg
     6181 ttaccgccac cggtattggt gaggatgatc aacgcgatag aagtaatcac cagctgcgaa
     6241 atgaccagcg ttaccggtac gccatttttg ttcattttag cgaatgccgc tggcagcagg
     6301 tttttctgcg ctgtgacata catcccgcga gaaggaccaa caatccagga ggcgatttcc
     6361 gccagaacac ccagcaacag cagtgcggag atcacgcgaa ccgtccactc aatttccggt
     6421 gccacatggg acatcagaac ggtaaaggtt tgcattaccc ctgcggagag gttgatttca
     6481 ttacccggaa tgaccatcgc aatagacaaa ccgccaacag agcttaagca gattgccgcc
     6541 accatcagca gtaacatagc cagtggatag tcgcgccctg ggttactcat ttcattgacg
     6601 tgggttgcgg aagcttctac gcccatataa ctcaaaatga aggcaacaaa tacaaccagg
     6661 gtgcccactt tagagaagtc agggaagaag gtcttcgaat ccatttcgat agcaacgggg
     6721 gcaccggagt gcagataaat agccgctaat gcaatcaaaa taaatgcagg taacaggata
     6781 ccggcgaaga agccaacttt agcaattcgc gccgtgtatt tcgtgccacc aaactgcgtt
     6841 aatgccagcg cccaaagaat gatgagtgct gcaatagttt tggtaatggg gtcttcattc
     6901 agcgctggcc atttcaggat gtaggagagt gcccctaaca cgaaatagag catcggaata
     6961 aaaccaatgg cgatttgcag atagccaaat gagatcgctg caaatcccca tctcggcccc
     7021 agagtatttg atacccaggc gaagacacca ccttcttccc agccgtcgac ggtggccatt
     7081 tccgcagcac aaagtcccac gggaataaac cataaaatcc cgcctaatag caggaagaag
     7141 actaatgaaa agcccgatgt tgcgaaggta gggtattcat aaacagccat taccatcgat
     7201 gccgttatgg caaaaaagcc gagtaatgtg agctgcttag ctttacctgt ctgtactaat
     7261 gtagccatat tatcccccta aaacggtatt cctgtcggaa ccgcacctgt tttgttctga
     7321 agcgtattca gaacaatatt ttccgttgct aatgccagtg aacagacttt ggaaattgtc
     7381 ccgaaacggg ttcgtttcgg gacaccgtta ccgttaaacg ttatcaggta tgtttaaagc
     7441 tgttctgttg ggcaataccc tgcagtttcg ggtgatcact gagatatttc agggaggctt
     7501 tgtagtcttc cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga cacataatgc
     7561 gcatcaccac gatgtcggtg gcttcaccgc cgagagtgaa ggccggaacc tgccagccgc
     7621 gcagacgcag acgttcagag aggtcataca gggtgtatcc cggatcttca ccatctttca
     7681 gtttgaagca aaccgccgga atgccttcgt ccgggcgacc ggtacagatg aactcatacg
     7741 gccccagttt ggcgatttca tccgccagat aagcggcaac ctggtaagag gcgttctgta
     7801 ctttggtata gccttcacga ccgaggcgca ggaattcata gtactgtgca attacctgac
     7861 ccgccgggcg ggagaagttg atggcgaaag taccaatctg accaccgagg tagtcaacgt
     7921 tgaacaccag ttcctgcggc agcgcttctt catcacgcca gataacccag ccgcagccca
     7981 gcggagccag accgaatttg tggcctgaag cactgatcga tttcacacgc ggcaggcgga
     8041 agtcccagac gatatccggg gcgacgaacg gtgccaggaa gccaccactg gcggcgtcga
     8101 tgtgcatgtc gatgtcgata ccggtgtcgg cctggaattt atccagcgca tcgtgcagcg
     8161 gttgcgggaa ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc acgccgatgg
     8221 tattttcgtc gcaggcttca atcatgcgtt tcggatccat aaacaactga ccggggcgca
     8281 tagggatctc acgcagctcc acatcccagt agcgggcgaa tttatgccag cagatttgca
     8341 ccgggccgca caccaggttt ggtttatccg ttggtttgcc tgcagcttcc atacgcttgc
     8401 gccaacgcca tttcatcgcc atcccgccga gcatacaggc ctcggaagaa ccaatggtgt
     8461 tggtgccaac ggcctgacca tttttcggcg caggcgcatg ccacagatcg gcaaccatat
     8521 ttacgcaacg caggtcgatg gctgcagatt gcggatattc ttctttgtcg atccagtttt
     8581 tgttaatgga taaatccatc aacttgtgga cgttttcgtc gtcccaggtc tggcagaaag
     8641 tggccaggtt ctgacgagcg ttgccatcaa gatataattc gtcattgata atctgaaatg
     8701 cgacatcgtc gcgcatttcg tgcagcggaa aacgttttga ttctgcgata
//
LOCUS       DBFIFS010000178         8744 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.178, whole genome shotgun sequence.
ACCESSION   DBFIFS010000178 DBFIFS010000000
VERSION     DBFIFS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.178"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..287
                     /locus_tag="N7A07_003679"
     CDS             <1..287
                     /locus_tag="N7A07_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HGU2478268.1"
                     /translation="QPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGIS
                     ESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            287..5557
                     /gene="traI"
                     /locus_tag="N7A07_003680"
     CDS             287..5557
                     /gene="traI"
                     /locus_tag="N7A07_003680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGU2478269.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQYNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDTVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMIFTPGSTVIVD
                     QGEKLPLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMRDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVSSV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKTRAGETSLETAISHQ
                     KSALHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVMPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGEIPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTEQLQSIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDNGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTREIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTSDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5577..6323
                     /gene="traX"
                     /locus_tag="N7A07_003681"
     CDS             5577..6323
                     /gene="traX"
                     /locus_tag="N7A07_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU2478270.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6378..6938
                     /locus_tag="N7A07_003682"
     CDS             6378..6938
                     /locus_tag="N7A07_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU2478271.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7069..7269
                     /locus_tag="N7A07_003683"
     CDS             7069..7269
                     /locus_tag="N7A07_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478272.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAAR
                     VAPRTDWQQFARQRAERCQRRR"
     gene            7655..8254
                     /locus_tag="N7A07_003684"
     CDS             7655..8254
                     /locus_tag="N7A07_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HGU2478273.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNN
                     VALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYD
                     GIVRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTF
                     NNYLKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
BASE COUNT         2108 a         2123 c         2820 g         1693 t
ORIGIN      
        1 aacagccagt gtcttctgtc atcaatgata agaagtcaga tgcaggtgtg agtgttccgg
       61 caggggggat cgagcaggag ctgaaaatga aaccggaaga agagatggaa cagcaactgc
      121 cacccgggat cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcaac
      181 aggaaaatca tccggacatc cagcagcaga tgcagcgtcg tgaagaggtg aacattaatg
      241 tgcaccggga gcgcggggag gatgttgagc cgggagatga tttctgatga tgagtatcgc
      301 gcaggtcaga tcggccggaa gtgccggtaa ctattatacc gacaaggata attactatgt
      361 actgggcagc atgggagaac gctgggccgg caagggggct gaacagctgg gactgcaggg
      421 cagtgtcgat aaggatgttt ttacccgtct tctggaggga aggctgccgg acggagcgga
      481 tctaagccgc atgcaggatg gcagtaataa gcatcgtccc ggctacgacc tgaccttctc
      541 cgcccccaaa agtgtctcca tgatggccat gttaggtggc gataagcgcc tgattgatgc
      601 acataaccag gccgtggact ttgctgttcg tcaggtggag gcgctggcct ccacacgggt
      661 gatgacggac ggacagtcag aaacggtact gaccggtaat ctggtgatgg cactgtttaa
      721 ccacgacacc agtcgcgatc aggaaccaca gttacacacg catgcggtgg tggctaatgt
      781 cacgcagtat aacggtgagt ggaagacact gagcagtgac aaagtgggga aaacggggtt
      841 cattgagaat gtgtacgcta atcagattgc ctttggcagg ctctaccggg aaaaactgaa
      901 agagcaggtt gaggcgctgg gctatgaaac cgaagtggtg ggtaagcacg gtatgtggga
      961 gatgccgggc gtaccggtgg aggccttttc cggacgctca caggctatcc gggaggccgt
     1021 cggggaagat gcctcgctga aatcccggga tgtggcggca ctggatacgc gaaaatccaa
     1081 acagcacgtc gatcctgaag tcagaatggc tgaatggatg cagacgctga aggaaaccgg
     1141 gttcgacatc cgggcatatc gtgacgcggc ggatcagcgt gcggagatcc gcacgcaggc
     1201 gcccggggct gtttcacagg aggggccgga tgtgcagcag gcggtgacac aggcgattgc
     1261 cggattaagt gaacgaaaag tgcagttcac gtatacggac gtgctggcca ggacggtcgg
     1321 catactgccg ccggaaaatg gtgtgattga acgggcacgc gccggtatcg atgaggccat
     1381 cagccgtgag cagcttatcc cccttgaccg tgagaagggg ctgttcacat ccgggattca
     1441 tgtgctcgat gagctgtcag tccgggcact cagtcgtgac atcatgaaac agaaccgggt
     1501 gaccgtacat ccggagaaaa gtgtcccccg gacggccggt tacagcgata ccgtcagtgt
     1561 tctggcacag gatcgcccgt cgctggccat tgtgtccggg caggggggag ccgccgggca
     1621 gcgtgagcgg gtggctgaac tggtcatgat ggcccgggag caggggcggg aagtgcagat
     1681 tatcgctgct gaccgtcgct cgcagatgaa cctgaagcag gatgaacggc tgtccggtga
     1741 gctgataacc ggacgtcgtc agctgcagga gggcatgatc ttcacgccgg gcagtaccgt
     1801 tatcgttgac cagggcgaaa aactccccct gaaagagacg ttaaccctgc tggacggtgc
     1861 cgcacgtcat aacgtacagg tcctgataac cgacagcggg cagcgaaccg gtacaggcag
     1921 tgcgctgatg gccatgaggg atgccggggt gaacacatat cgctggcagg ggggagaaca
     1981 gcgaccggca accatcatca gtgaaccgga ccgtaatgtc cgctatgccc ggctggccgg
     2041 ggattttgcg gccagcgtga aagccggaga agagagcgtg gcacaggtca gcggggtacg
     2101 ggaacaggcc atactgacac aggccattcg cagtgagctg aaaacgcagg gcgtgctcgg
     2161 acatccggag gtgaccatga ccgcgctgtc accggtctgg ctggacagcc ggagtcgtta
     2221 tctgcgggat atgtaccgcc cggggatggt gatggagcag tggaacccgg agacacgcag
     2281 tcatgaccgc tatgtgatag accgggtgac ggcgcagagt cacagcctga ccctgcggga
     2341 tgcgcagggc gaaacgcagg tggtgcgtat ttcttctctg gacagcagct ggtcgctgtt
     2401 ccggccggaa aaaatgccgg tggcagacgg cgagcgtctg agggtgacag ggaaaattcc
     2461 cggactccgc gtctccggcg gtgaccgcct gcaggtgtcg tccgtcagtg aggacgcgat
     2521 gacggttgtt gtgccgggac gggctgaacc ggccaccctg cctgtgagcg attcaccgtt
     2581 cacggcactg aagctggaga acggctgggt ggaaacgccc gggcattccg tcagcgacag
     2641 tgcgacggtt tttgcctccg tcacacagat ggcaatggac aatgccaccc tgaacggtct
     2701 ggcccgcagt ggtcgtgatg tccggctgta ttcctcactg gatgaaaccc gtactgcgga
     2761 aaaacttgcc cgccatccgt cctttacggt ggtttctgag cagataaaga cgcgggccgg
     2821 tgagacatcg ctggaaacgg ctatcagtca tcagaagtcc gcacttcaca cgccggcaca
     2881 gcaggccatt catcttgccc ttccggtggt ggaaagtaaa aacctggcct tcagcatggt
     2941 ggacctgctg acagaggcaa agtcgtttgc tgcagaagga accggtttta ctgaactggg
     3001 aggggaaatc aatgcgcaga taaaacgggg tgatttactg tatgtggatg tggcaaaagg
     3061 ctatggcaca ggtctgctgg tttcccgtgc gtcgtatgag gcagaaaaga gcattcttcg
     3121 ccatattctc gaaggtaagg aggcggtcat gccgctgatg gagagagtac ctggcgaact
     3181 catggagaaa ctgacatcag gacagcgtgc cgccacccgc atgatactgg aaacgtccga
     3241 ccgtttcact gtggtacagg gctatgccgg tgtgggtaag accacacagt tccgggcggt
     3301 gatgtcagcc gtgaacatgc tgccggagag tgagcgtccc cgcgtcgtgg ggctgggtcc
     3361 cacacaccgt gcggtcgggg agatgcgcag cgccggcgtg gatgcgcaga cactggcgtc
     3421 ctttctgcat gacacgcagc tgcagcagcg cagcggagaa ataccggatt tcagcaacac
     3481 gctgttcctg ctcgatgaga gctcaatggt gggcaatacc gacatggcac gggcatatgc
     3541 cctgattgcg gccggtggcg gtcgggctgt ggccagtggt gacacggagc agctgcagtc
     3601 catcgcgccc ggtcagcctt tccgcctcca gcagacgcgc agtgctgccg atgtggccat
     3661 catgaaggag attgtgcgtc agacgccgga actgcgggag gcggtataca gcctgattaa
     3721 ccgggatgtg gaaagggcac tgtccgggct tgagagtgtg aaaccgtctc aggtgccacg
     3781 tcaggagggc gcatgggcac cggagcactc cgtgacggag ttcagtcaca gccaggaagc
     3841 gaaactggca gaagcgcagc agaaggcgat gctgaaaggc gaggcttttc ctgatgtccc
     3901 catgacactg tatgaagcca ttgtccgcga ctataccggc aggacaccgg aagcacggga
     3961 gcagacgctg attgttacgc acctgaatga agacaggcgc gtactgaaca gcatgattca
     4021 tgatgcacgg gaaaaggccg gtgagctggg aaaagagcag gtcatggtgc ctgtcctgaa
     4081 cacagcgaat atacgcgacg gggagttgcg tcgtctctcc acctgggaga cacatcggga
     4141 cgcacttgcc ctggtggata atgtgtatca ccggattgcc ggtatcagta aggataacgg
     4201 gctgataacc ctgcaggatg cggaaggtaa cacgcggctg atttcgcccc gggaggcggt
     4261 ggctgaaggc gtcacactgt acactccgga caccatccgg gtgggaaccg gtgaccggat
     4321 gcgcttcacg aagagtgacc gggagcgcgg ttatgttgcc aacagcgtct ggacggtgac
     4381 agcagtttcc ggtgacagtg tcacgctgtc ggacggacag cagacccggg agattcgccc
     4441 cggccaggag caggcagagc aacatattga cctggcctat gccatcaccg cccacggtgc
     4501 gcagggtgcg agtgaaacct ttgccattgc gcttgaaggc acggaaggta accggaaact
     4561 gatggccggc tttgagtcag cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt
     4621 gtacaccgat aaccgtcagg gctggacgga tgccattaac aatgccgtac agaaaggaac
     4681 tgcccacgat gtgctggagc cgaaaccgga ccgggaggtc atgaatgcag agcggctgtt
     4741 tagtacggcg cgggagctgc gggacgtggc ggcagggcgt gccgttcttc gtcaggcggg
     4801 gcttgccggg ggagacagtc ctgcacggtt tattgcaccg ggacgtaaat atccacagcc
     4861 gtatgtggca ctgccggcgt ttgaccgtaa cggcaaatca gccggtatct ggctgaaccc
     4921 gctgacctcg gatgacggaa acgggctgcg gggattcagt ggtgaaggcc gtgtgaaagg
     4981 cagcggggat gcgcagttcg tggccctgca gggcagccgt aacggagaaa gcctgctggc
     5041 tgataatatg caggatggtg tccggattgc ccgtgataat cctgacagtg gtgtggtggt
     5101 aagaatcgcc ggtgaaggcc gtccgtggaa tcccggtgcc atcaccggtg gccgcgtgtg
     5161 gggggatatc ccggacaaca gcgtccagcc gggagccgga aatggcgagc cggtcacggc
     5221 agaggtactg gcacagcggc aggctgaaga ggccatccgc cgtgaaacgg aacgccgtgc
     5281 ggatgaaatt gtccgtaaaa tggcagagaa caaacctgac ctgccggatg gcaaaacaga
     5341 gcaggctgtc agggagattg ccgggcagga gcgtgaccgg gctgccataa ctgaacggga
     5401 agccgcgctg ccggagagtg tactgcgtga accacaacgg gagcgggaag ctgtccggga
     5461 ggttgcccgg gaaaatctgc tgcaggagcg tctgcagcag atggagcggg atatggtccg
     5521 tgacctgcag aaagagaaaa ccctgggcgg agactgatac aggaagataa acgctgatga
     5581 caacggataa cacgaacacg acacgtaacg attcactggc tgcccggacc gatacctggt
     5641 tgcagtcgtt gctggtctgg tcacccggac agcgggatat catcaaaacg gtggcactgg
     5701 tgctgatggt actggaccac atcaatctga tattccagct gaagcaggaa tggatgtttc
     5761 tggccgggcg tggagccttt ccgctgtttg ccctggtgtg ggggctgaat ctgtcccgtc
     5821 atgcgcatat ccggcaaccg gccattaacc ggctgtgggg atgggggatt attgcccagt
     5881 tcgcatatta tctggcaggc tttccctggt atgaggggaa tatcctgttt gcctttgcag
     5941 tggcggcaca ggtgctgacg tggtgtgaaa cgcgcagtgg gtggcgtaca gcagcggcca
     6001 ttcttctgat ggcgctgtgg gggcctttgt ccggcaccag ttacggcatt gccgggctgc
     6061 tgatgctggc agtcagccac cggctgtacc gggcggaaga cagagcggaa cgtctggcgc
     6121 tggtcgcctg cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg
     6181 cggtggccgg tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat
     6241 cattaccccg tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg
     6301 tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg accgcaagag gatgattcgt
     6361 cagaggcaga acacagcatg acagagcaga agcgaccggt actgacactg aagcggaaaa
     6421 cggaagggga gacgcctgtc cgcaggcgga aaaccatcat caatgtcacc acgccaccaa
     6481 agtggaaggt gaaaaagcag aagctggccg agaaagcagc ccgggaagca gagctggcgg
     6541 caaaaaaagc gcaggccaga caggcactgt ccatttatct gaatctgccc acgctggatg
     6601 aggccgtgaa taccctgaaa ccctggtggc cgggattgtt tgacggtgac acgccccggc
     6661 ttctggcctg cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc
     6721 tctcgcataa aaaactgcgc agggcgctga aggccatcac ccgttcagaa agctatctgt
     6781 gtgccatgaa agccggtgcc tgccggtatg acacggaagg gtatgtgacg gagcatattt
     6841 ctcaggagga ggaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaaccgga
     6901 taaaggcaga acttcaggcc gtgcttgatg agaaataaaa aaagcctcca tcagagagga
     6961 ggcagggaaa taaggcttaa aaggaaataa cttccgtacc cgaaaggaag ccaattcagt
     7021 agattattta acaccataat actgacgggt taaagaggag tgataaagat gaatggattc
     7081 agaaacagtt cacggaacgg ccaggtctgg cgctggcagc gtgccggaag ccgtgccgtc
     7141 agcctggagg tcaggggatt ccggaaggag gccgctgaag cgtggctcct ggctgcccgt
     7201 gttgctcccc gtacagactg gcagcagttt gcacggcaga gagccgaacg ctgtcagcga
     7261 cggagataat gaaaaaaaac ggcaaaaaaa agacttcccc tgagcaaagg aggccaaaaa
     7321 tcattcatag cgtatcactg acattgaatg aatgctctgt acatcaaccc tgtgaagcta
     7381 ccaccggtta atgccgggag gttgcgggaa gaaagataac acacagtcat tacgtggtta
     7441 atatctgtca gaatgttttt tgtgaccata aactgtcaga tggataaaaa agaccgtcat
     7501 gtgacggtca gtgttgtcct gtcagggaca aatgcattca gtaaggagag caaaataata
     7561 acatcacgtt gcccggctgt taatatcagg cgatattaag tgttatttat gactctttta
     7621 tctggttgtg aagtctgtta atcaggaaga gaaaatggcg accggaaaat cagagaagca
     7681 acaggtgatt gcctttatcg attacgagaa ctgcagtaac ctgacagaaa tttccttaaa
     7741 tatatatacg gagttaatta tattcgcggg tgcgaaacag aataatgtgg cactgccagt
     7801 aaacgctttc cctgaagcag taaaaatcac actccggcat gtcagtgaag tctcccggaa
     7861 caatgttgat tttcatctgg tgctggagct ggggcagtgt gtatgccact acgggccgga
     7921 gaaggaatac catattgttt ccgctgacaa gggatatgac ggtattgtcc ggacactgca
     7981 ggcgcgcggg attcgctgcc gtcgcgtctc cccggataag gcgtcatcag taaagatggc
     8041 tgcacccgac atggatatcg ttatccggtg ggcaaacaaa attcaacagg cagcgcggga
     8101 ggttcgtaat atgccggtaa cagaaaagac atttaataat tatttaaaat cacagatgcg
     8161 cggtgaatgg cgtgatgtcc tgaccagagg tatccgggat gaactggtcc gtcgcggggt
     8221 aatgaaaatt caggggaata aaatcatctg gtaagactgc cagaataaaa tgatatttat
     8281 tttatttata aaaacagaaa ggtcgaaaat caataatggc atttatttcc atgacaagac
     8341 agaaagcgcc gtacagatat gcaagcaaga cccggttatc gataagacga acagactcag
     8401 gctctgccgg gtgttatgtg acggttgccg ttcgggggaa gcaggaattt attattgctg
     8461 aacttgattc agaaatccgg aaaatcaggc tgaaactgac ggactcctat gaagaagggg
     8521 taaggctgtc ttcgggcacg tttacgcttc cggccagatt ctgccgggaa atactgccgg
     8581 acgacgtcag gagtatcacc attattctgg aaaaaagcga cgatgagtgg tggtacggga
     8641 gttactgata tgtggcagaa ctccggacac tgacgccttc gcgttgctca gttgtccaac
     8701 cccggaaacg ggaaaaagca agttttcccc gctcccggcg tttc
//
LOCUS       DBFIFS010000179         8717 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.179, whole genome shotgun sequence.
ACCESSION   DBFIFS010000179 DBFIFS010000000
VERSION     DBFIFS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.179"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(39..350)
                     /locus_tag="N7A07_003685"
     CDS             complement(39..350)
                     /locus_tag="N7A07_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478274.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(343..570)
                     /locus_tag="N7A07_003686"
     CDS             complement(343..570)
                     /locus_tag="N7A07_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478275.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            complement(567..848)
                     /locus_tag="N7A07_003687"
     CDS             complement(567..848)
                     /locus_tag="N7A07_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2478276.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            complement(881..1597)
                     /locus_tag="N7A07_003688"
     CDS             complement(881..1597)
                     /locus_tag="N7A07_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2478277.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(1631..2092)
                     /locus_tag="N7A07_003689"
     CDS             complement(1631..2092)
                     /locus_tag="N7A07_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2478278.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(2085..3128)
                     /locus_tag="N7A07_003690"
     CDS             complement(2085..3128)
                     /locus_tag="N7A07_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2478279.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            complement(3197..3622)
                     /locus_tag="N7A07_003691"
     CDS             complement(3197..3622)
                     /locus_tag="N7A07_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2478280.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(3606..3848)
                     /locus_tag="N7A07_003692"
     CDS             complement(3606..3848)
                     /locus_tag="N7A07_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2478281.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            4240..4578
                     /locus_tag="N7A07_003693"
     CDS             4240..4578
                     /locus_tag="N7A07_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU2478282.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            4871..5023
                     /locus_tag="N7A07_003694"
     CDS             4871..5023
                     /locus_tag="N7A07_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2478283.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            5035..5673
                     /locus_tag="N7A07_003695"
     CDS             5035..5673
                     /locus_tag="N7A07_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478284.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(5674..5883)
                     /locus_tag="N7A07_003696"
     CDS             complement(5674..5883)
                     /locus_tag="N7A07_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478285.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            6448..6636
                     /gene="dicB"
                     /locus_tag="N7A07_003697"
     CDS             6448..6636
                     /gene="dicB"
                     /locus_tag="N7A07_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2478286.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            6633..6821
                     /locus_tag="N7A07_003698"
     CDS             6633..6821
                     /locus_tag="N7A07_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2478287.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            6914..8152
                     /locus_tag="N7A07_003699"
                     /pseudo
     CDS             6914..8152
                     /locus_tag="N7A07_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            8140..8691
                     /locus_tag="N7A07_003700"
                     /pseudo
     CDS             8140..8691
                     /locus_tag="N7A07_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2263 a         2259 c         1946 g         2249 t
ORIGIN      
        1 aaatcttcag tatttagttg ttagttggtt tatcgccttt atgcttcagc cttatttcgc
       61 aaccagacac aaaccgggcc atcttccgta tcatgaatgg aaccaataaa ccagccatcg
      121 ccctctggtc gttccggttc ccatgcagaa atatcagcat cacacgcatc aaggtcagca
      181 catccttcat ctctgaagca gaggacgtat tgaagattat tttcctccat ccaggcgtta
      241 aactcttccg ttgaaatata ttcccgacca tcacagaatt tttcatattc aggatgtgtc
      301 cagtagccat atccgtcacg aactaccggc atttctttaa tttcactcac tgttaacctc
      361 ctgcaacgct acacgatacg ccttctttat ccacgcctta ctgccatata atttcgtctt
      421 cataataaac acacctgcac gactcgccga tatccccgga caggttaaca gcacagcatc
      481 caccacacgg ttatgcttcc gaaactccat tacagtactg ctgataacca cctgccccac
      541 cgggccgtaa tcctgataca ggattttcac gcagacaccc tcctgtcgaa ataaacgtag
      601 ttattcacta tgcgcagcgg catgcctaat tttctggcaa tttcccttct ttgcatgcct
      661 ctctgatgca gttgccgcgc cagctcaata tcacgctgag aatattttgc cgacgggtga
      721 aaatcaccac gtaacatcat gctgatgccc agctcccgtg ccttcgttct cactgccgct
      781 tcagttcgtc cgataagtgc gccaacgctt tttaccttca tcgttcccgc acactgccgg
      841 agtatcatga tttccgcccg gcaccacgtc ttccacccac tcaccgctgc tgttctctgg
      901 tggcggtaat atcccggaga atatcccggc acttgttcag ctcccgcagc gcggcgcaga
      961 ctcgctccca cttctgaacc tgaccttttg cccggcgcag ctcgcggtta gccacatgca
     1021 gcgatggtaa aatcagacca tccggatgct ttctggtgaa cgacggctgt gactgcactg
     1081 tgaccgccac actttccgtt tttatttctt cctgtgtttc cgcttccccg actggtaacg
     1141 caacacctgc tggctgagga aaggctttac catccgtttc cgttatggat gcggctttcg
     1201 gctctgccgg taaatcagcg cccggtatgc agtaccgaaa tttaccgccc tgattcacgc
     1261 gaatcagacg ccctttgctg attgccatgg ccagtgatga attcgcccgg cgggaggtaa
     1321 tcccgaacat cagtgccagc tcatccgccg tttgtgggcc atgatgttca atcgcctcag
     1381 tcagcatttg cgctgtcact ttcggtaccg gtgacactgg ttcactttca ccagtctgag
     1441 tcagccacca catcgacccc ttgttatccg cttcaccacg gcgcttcagt ttccacagtt
     1501 cgttgacagc atcttcgcgg ctgattccaa ggcgcgatgc cactacctgt gaagaggctt
     1561 ttttcagtgc tttcagtgcg tcaaatacgg tttccattaa tatttcctcc gacaaaatcg
     1621 tttctcagat tcaaataaaa ccagctgcct tccggcgttc gtattcctgt ttcagccgtt
     1681 caattggcgt tggcccttgc gggtgtttcg ccccttccag ttgtcgtcgc actggcggaa
     1741 cactcatccc gttaccaaca tgctttgccc atttcgtcag ttgccgttcc gcaagtcgtt
     1801 ttaactcacc ttgcgtcatc tggcgctcaa tccctctggt acgcatttcg aggcagatgt
     1861 ggtacagcac aggctgtggc cacgggtatt tatcactccc gtcgtatcgc caggattcat
     1921 tgcgccagcg ccggtactct tccatcacgg catccaccgt aagaccaaat ggatttgccc
     1981 cactctccga aatcagcgca acaaactcag ccaggtccgg gggccacgtt tcacccgccc
     2041 ggcagcggtc catgcactga cggcacacca gacggatttg ctgttcagtc atcgcaccaa
     2101 tctgggcaat ccagagcttc gaaggtgcgg ccccgttctt ctgagtccag cggttcgaat
     2161 acacctcccc catgagctcc cacagcttcc aggccgtttc cgttgctgat aaatccgttg
     2221 tcacgttccc actgttcgcg tgcagcccgg atttcctgaa ctgcccgtga tgccgtgcca
     2281 cctgatgctg catggcttcc ccccttgctg actggtttta cctgtgccct gacgtgctgc
     2341 acgtggcggg caaatttctg ctcccactga acctgcgtga aaaccttccc ctccgccatc
     2401 cagtaatccc ggaatgcggc aagctcagca ggtgtaaatt ccggctcagg cagagccata
     2461 ccccacactg ctgcccgttg tcgaaaatcc ggcgacggct gccagacagt agtcatcgaa
     2521 aatttcccga tcggttcgct caggccgtcc aggtattcag gttcggctgt ctgcaacggc
     2581 gcaccattcg actcactggt cggaatactc tcgcgcgcgt tatgtgtggg gtttaattct
     2641 gtatctgtat ctttatctgt atctttatct gtcgtgactt gtcgtgacag atgcgtgaca
     2701 cgtcgtgact catcgtgaca atcagcatta tgtttccgca gcttttcccg ctcccgctgc
     2761 gctctcttgc gctctgccgg ggattttgcc gtttgcgaaa cgttaccatt gtcctctttc
     2821 agcacctgac gtttttccca tccggaaata aggtcaccat ccagaacccg cccctgcatt
     2881 gcatgcaaaa ttgaatcaat tacgtcttcc gtcacatcaa gcgcacttgc taaatcttcc
     2941 gtcgtgacat caatgtgacc acgtagtgac acgccgtgac atgtcgtgac atttcgtgac
     3001 gcgctcacca gaagatggat atacactgcc atcactgttg cgattggctg tcctgatacc
     3061 ctggcaatcg ttcgccactt ggggtcattt ggcatgtcat gccacaatct gagccaggca
     3121 ttagccatac tcacctcttc tgataccgaa ctttaccctc gaacatccgg aagaaatccg
     3181 gcatgaatat tgttggtcaa tgcacgacaa cagcattacc aggctgaccc ccactgttag
     3241 tcagggtgcc ccaggcgatc gccgctgcga caaaatcatc cacatctttc accagccgat
     3301 ccctccgttc gacgatctca cggtaatatt cagagctgtg actgcgcata cgggccacca
     3361 gcagaggcgg cattgccttt tcgatcgccg gtaacagagc ctgcattttt tcaactgcat
     3421 caggggtgtc tttctctatc cagcggaaaa ttttctgggt attgcgagcc agggcttccg
     3481 gatggctgtc gtcgtacagt tcaggaaacg tcatacccaa ctcaaaataa gcctgggtta
     3541 ttccagctgc cggaactttt tcgccatcag gacgcgccca ggcattcatc gccatgcgga
     3601 tgtgttcatg cttgattttc atgaatcccc ccttggttag aaggcggatt atgatcagaa
     3661 tcgggaatga caaccgtcgg tatgtgtaac tcatatttga gcgccccggc agtgactgcc
     3721 tgaattagca acgcccattt ccacggaacg tcttcccccc acatgctgac tgtggttttt
     3781 gacgttccta gagcggcggc tgttttaaca actccgccaa aatagcctaa tacttctgat
     3841 tttttcatga gtcgctccat aaaactgaac gccaaaagtt taataatcaa aaccaaagaa
     3901 agtcaagaaa caaaaccatc tgtgttttaa aatcaaaaca tgaacaagca aacaatatct
     3961 gaacgcataa cccaacgtat gcatgcgcta aacttgaaag gcaaagacct tgtcaatgcc
     4021 actggcgcat caaaaggctc cgtaagtcaa tggatgaacg gtggaggagc gccgtcctcg
     4081 cgttacataa gttcactggc aaagatattg aaagtaaacg aaaattggct tcttaatgga
     4141 ggagagttaa atacaggtga ttcgcttgat ctatctttac cgccgataaa aacggttccg
     4201 ctactatcac ttcagcaggc agcaagctgg agtgattata tgaaaaattc ctcaataacc
     4261 tcttgtgtgc agcttgtcgg agaaatcccg gccaatacct ttgctgttgt tctagagagt
     4321 gacagtatgt cgacatctgg tggtggagtt tccatcccaa atggttcaac agtttttgtt
     4381 gatcccgatc gaatcgtaca accaggaaat attgtccttg ccttacccaa agggaccaca
     4441 acgcctgtca ttcgcaaact ggagatagaa gggccggata ttcttttagt ccccacgaat
     4501 cctcgctacc cttcaattat gctggatgat ctatcttgca tattgggagt atgctttaaa
     4561 attcaacaag atatttaacc aacctcatct atttgattaa ctgtatgcca tcgtagtgat
     4621 ggcgtaacag ctgcctgctt aaaatgtttt gataaaaaaa cattgacctt aaatgttcgt
     4681 ttttctaaac ttcattcatt ccctcacccc gccccacaga atgcagggca atacttcgag
     4741 ttaccaggca gtggtcaggg gttaagtagc cagcccgagg cgtaagaaca tgacggcagg
     4801 gttcaacttt aataactatg cagcaggttt ttgttccgct accccggcgt taaggggaaa
     4861 tgaggtcaac atggatacgc tcaatcttgg caacaacgaa tctctggtat gtggcgtgtt
     4921 ccccaaccag gacggtacgt tcaccgcgat gacgtatacc aaaagcaaaa cgtttaaaac
     4981 tgaagctggc gcgcgtcgct ggttagccag aaacactgac tgatgaggtt gacgatggaa
     5041 tttaaagatt tacctccttc aatccaggag attgcagcac acacacttcg tcatcgtctg
     5101 aacgaacttg aattggaatc ggtaacgaaa aaagacactg ataatatggc tcgtaatgtg
     5161 cgcgatgcgt ttaccggatt gtatttctgt gcgtctataa ataaacacga ctcagagagt
     5221 gtggcaaata aaattgcaga aacgacagcg caaaacatca atacgaaacc aacggaagaa
     5281 gaaattgatc agtttgctca tgatgctggt ttaaaaaaca agaaagaaaa atcgccatat
     5341 gcggggaaca tgtttgttta tgacaatctc atcagaattc gtggcgaaat tccggcggaa
     5401 tacctggcaa gagtccatca ggcattgctt aaaaatttgg aaacagaatt atttgatggc
     5461 aacactaacg gtttcttcat ggtatcaggc cttgagaaag actgggatgc agaaaaacgc
     5521 tggaatgttg ctacatggtt attcagtaac agagccgctg cactggaagc ttcggcatgt
     5581 atttgcggcc tgttcttaac agaccacaaa tataatctgg atgtgtacag ttatatttac
     5641 gctgaacacg gtccactctg gattgactgg taattataag gaaacaccag cagggccgcg
     5701 gcgaccaaca gaacgattaa aatcaataat gccattataa aggacattat ttaatttatc
     5761 gtcgaatgct gattctgtga gcctcaactc tgaatgagtt tttaataacc ctgattgcct
     5821 gagttgattt accaggcatt caatctgttt ttcaataagc ggatttcttt ttttgtttgg
     5881 cattttatcc tccattgagg ttctgggtta aaaatggaga ccaacacgct gtcacgtgtg
     5941 gtcgtgcgcc ggacacggat aagaatccgg tactgacagt ttactgaaag gatatttccc
     6001 tgaaaagtca gtgcataacg cgaaagcgta cggcgaagct ctttccctta gaaggcttgt
     6061 cgttagattt cttcgaacgt gcgcttccgg ttgtggcact ccgcgaaatg gcgcggcggt
     6121 aagtatggcg gggttattcc ttccccattg aggacaccgg gttgtcaggt tgaccatacg
     6181 cttaagtgac aaccccgctg caacgccctc tgttatcaat tttctggtga cgtttggcgg
     6241 tatcagtttt actccgtggc tgctctgccg ccctttttaa agtgaatttt gtgatgcggt
     6301 gaatgcggct aagcgcacgc ggaacagtta aaaccaaaaa cagtgttatg ggtggattct
     6361 ctgtatccgg cgttaattgt taactggtta acgtcacctg gaggcaccag gcactgcatc
     6421 acaaaattca ttgttgagga cgcgataatg gaaacgttat taccaaacgt taatacgtct
     6481 gaaggttgtt ttgatattgg tgttctgctc agtaaccggg agtttactga agatgccatt
     6541 aacatgagga aatatgagcc ttatctgctc aatgataatt ccatactttc ccgaattgct
     6601 cttcttgaac ttggtatttt cggagaacgt caatgacttc agcatttgca ctgatgatga
     6661 cggtttttct tataacgggt gaatcacaga atgtgattac cggaatttat gccagtaaag
     6721 aatcctgcct ccaggcaaga gacgagcaaa aaatttctgg tgaatgcctc ccggtaaaaa
     6781 aagtatcgct gtacctgaat aacgaaacac cggctggata accctccagc catattaaca
     6841 ccataccaac ggattaaaaa tgccagcaat ggcagggatt tgttcaccct taaatctgta
     6901 atgaggttta tcaatgagca ctgataaaga agaatttgcg ctatattgcg aagcaaaaaa
     6961 tgacaaagtc agaaaacgtc tgggaattaa aggtggtttt tactggacta cagcaaaaaa
     7021 attatctgtt gccatctccc gctgcattac cgcaatggat gacaacgatt atgatgaaga
     7081 cgactttaaa aaacccgttc gcgtccattt acccgttgtg aatgaccttc cacctgaagg
     7141 cgtgtttgat accgaattct gcaaccgata cgaaaaaggc ggggaagatg gcattacaat
     7201 ggtatttatc gcgccctctc cctccgtgca ggagaaacca gccagtactg acaataccaa
     7261 cgtcaacggc gaagacatga cggagattga ggagaatatg ctcctgccgg tttctggtca
     7321 ggagctgccc attcgctggc ttgcgcaaca cggcagcgaa aaaccagtaa cgcacgttgc
     7381 acgggaagaa cttcaggcat tacatatagc acgggcggaa gaactgccgg ctgttactgc
     7441 cctggccatt tctcacaaaa caaagctgct cgacccgctg gagattcgcg accttcacaa
     7501 actggtacgc gacactgaca aagttttccc taatcccggt aattcagacc tgggactgat
     7561 aactgctttt ttcgaggcat acctggacgc tgactacact gatcggggtc tgctgacaaa
     7621 agagtggatg aaaggaaatc gtgtttcgcg tatcacccgt acggcttccg gtgcaaatgc
     7681 cggtggcggg aacaaaaccg atcgcaatcc gaatttagta cacaccttcg atacgctgga
     7741 tgtggagatt gcagcagcca cacttccgat ggattttaat atttatgaaa ttccgggcag
     7801 cgtttatcgt cgcgcaaaag aaatcgtcct gaaaagagaa agtccgttca aagaatggtc
     7861 cgcagcactt cgcgcaaccc cgggtattct ggactattcc cgcgccgcta tttttgcact
     7921 tatccggagc gctcaccctg agttttatca ctacccggga cgccttcagg ggtatatcaa
     7981 cgcctactta acggagactg atcacgagaa ccccagcaag gaaactctca ctgctgcacg
     8041 acatacaccg gaaaaagata tcctggaaga agttaaccgc gaactgtctg ctaagcagga
     8101 aacagaagaa gaaaatgatg aagaaaaacc gcaaccatct tgcgcaatgg cagaatgata
     8161 atcagaagaa atctggcaaa agaccgtttt acagtaccaa aaacacaggc gggaaccaat
     8221 cgtgtgattc accttattaa gcccgcaatc gacgctctcc ggagtcagat ggcactaacg
     8281 agactgagca aagagcatat catcgatgtt cacctcagag aattcggcag aacagagaaa
     8341 caaaaatgta cctttgtttt tcaacctgaa gtgtcagcga aagtaaaaaa ttatggcgac
     8401 cattttaccg ttgactcaat aaggcagatg tgggacgcag cggtaaagcg tgccggaatc
     8461 cgccatcgca aatcgtatca gtcgagacac acttatgcct gctggtcgtt aacagcagga
     8521 gctaacccgg cattcattgc aaaccagatg ggccatgcag atgcgcaaat ggtgtttcag
     8581 gtgtacggga aatggatgtc agaaaataat aatgcgcagg taacgctgtt aaatacacag
     8641 ttaagcgagt ttgccccaac catgccccat aacgaagcga tgaaaagtta attttttatt
     8701 tatcaattag ttagatt
//
LOCUS       DBFIFS010000180         8685 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.180, whole genome shotgun sequence.
ACCESSION   DBFIFS010000180 DBFIFS010000000
VERSION     DBFIFS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.180"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(351..614)
                     /gene="rpsT"
                     /locus_tag="N7A07_003701"
     CDS             complement(351..614)
                     /gene="rpsT"
                     /locus_tag="N7A07_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU2478288.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            717..941
                     /locus_tag="N7A07_003702"
     CDS             717..941
                     /locus_tag="N7A07_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU2478289.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            949..1890
                     /gene="ribF"
                     /locus_tag="N7A07_003703"
     CDS             949..1890
                     /gene="ribF"
                     /locus_tag="N7A07_003703"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU2478290.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1933..4749
                     /gene="ileS"
                     /locus_tag="N7A07_003704"
     CDS             1933..4749
                     /gene="ileS"
                     /locus_tag="N7A07_003704"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU2478291.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4749..5243
                     /gene="lspA"
                     /locus_tag="N7A07_003705"
     CDS             4749..5243
                     /gene="lspA"
                     /locus_tag="N7A07_003705"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU2478292.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5331..5780
                     /gene="fkpB"
                     /locus_tag="N7A07_003706"
     CDS             5331..5780
                     /gene="fkpB"
                     /locus_tag="N7A07_003706"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2478293.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5782..6732
                     /gene="ispH"
                     /locus_tag="N7A07_003707"
                     /gene_synonym="lytB"
     CDS             5782..6732
                     /gene="ispH"
                     /locus_tag="N7A07_003707"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU2478294.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6798..7712
                     /gene="rihC"
                     /locus_tag="N7A07_003708"
     CDS             6798..7712
                     /gene="rihC"
                     /locus_tag="N7A07_003708"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU2478295.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7879..>8685
                     /gene="dapB"
                     /locus_tag="N7A07_003709"
     CDS             7879..>8685
                     /gene="dapB"
                     /locus_tag="N7A07_003709"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU2478296.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLD"
BASE COUNT         1987 a         2166 c         2461 g         2071 t
ORIGIN      
        1 gattgctgtt aataatttac aaggtctaat gaggagatat ttatgaatgg ttaaagtata
       61 taggaattta attcaaaaat agagtggtct taagtttaat acctcaatgt gtatcacaat
      121 atccacattc tttgtagggg agtctggaga ttgagtagat attcttgttc agaatgtatc
      181 agccgatggt tctacgattc ttaagccacg aagagttcag atagtgcaac ggcatgtctc
      241 ttttgactat ctggcaaccg gcagtgtgtt ctctcacgca tcacaaaagc agcaggcata
      301 aaaaaacccg cttgcgcggg ctttttcaca aagcttcagc aaattggcaa ttaagccagt
      361 ttgttgatct gtgcagtcag gttagcctta tgacgtgcag ctttgttttt gtggatcaga
      421 cctttagcag cctgacggtc cacgatcggt tgcatttcgt taaatgcttt ctgtgcagca
      481 gctttgtcgc cagcttcgat agctgcgtat actttcttga tgaaagtacg catcatagag
      541 cgacggcttg cgttgtgctt acgagccttt tcagactgaa tggcgcgctt cttagctgat
      601 ttgatattag ccaaggtcca actcccaaat gtgttctata tggacaattc aaaggccgag
      661 gaatatgccc ttttagcctt cttttgtcaa tggatttgtg caaataagcg ccgttaatgt
      721 gccggcactc gttacgtagt gatggcgcag gattctacca gcttgcgggg tgtgaataca
      781 gcttttccgc gataaaaatt gcagcaggca gtcagtttct tcccgtgatt tgcgccatga
      841 ccatgacaat gaaaagccac ttctttctga tttcggtact caatcgccgg ttaaccttga
      901 ccgctgtaca aggtatactc ggacgatttt cactgttttg agccagacat gaagctgata
      961 cgcggcatac ataatctcag ccaggccccg caagaagggt gtgtgctgac tattggtaat
     1021 ttcgacggcg tgcatcgcgg tcatcgcgcg ctgttacagg gcttgcagga agaagggcgc
     1081 aagcgcaact taccggtgat ggtgatgctt tttgaacctc aaccactgga actgtttgct
     1141 accgataaag ccccggcacg gctgacccga ctgcgggaaa aactacgtta ccttgcagag
     1201 tgtggcgttg attacgtgct gtgcgtacgt ttcgacaggc gtttcgcggc gttaaccgcg
     1261 caaaatttca tcagcgatct tctggtgaag catttgcggg taaaattcct cgccgtgggg
     1321 gatgatttcc gctttggcgc tggtcgggaa ggcgatttct tgttattaca gaaagctggc
     1381 atggaatacg gcttcgatat caccagtacg caaacttttt gcgaaggtgg cgtgcgtatc
     1441 agcagcaccg ccgtgcgtca ggcccttgcg gatgacaatt tggctctggc agaaagttta
     1501 ctggggcacc cgtttgccat ctccgggcgt gtagtccacg gtgatgaatt agggcgcact
     1561 ataggtttcc cgacggcgaa tgtaccgcta cgccgtcagg tttccccggt gaaaggggtt
     1621 tatgcggtag aagtgctggg cctcggtgaa aagccgttac ccggcgtggc aaacatcgga
     1681 acacgcccaa cggttgccgg tattcgccag cagctggaag tgcatttgtt agatgttgca
     1741 atggaccttt acggtcgcca tatacaagta gtgctgcgta aaaaaatacg caatgagcag
     1801 cgatttgctt cgctggacga actgaaagcg cagattgcgc gtgatgaatt aaccgcccgc
     1861 gaattttttg ggctaacaaa accggcttaa gcctgttatg taatcaaacc gaaacacgga
     1921 accgagaatc tgatgagtga ctataaatca accctgaatt tgccggaaac agggttcccg
     1981 atgcgtggcg atctcgccaa gcgcgaaccg ggaatgctgg cgcgttggac tgatgatgat
     2041 ctgtacggca tcattcgtgc ggctaaaaaa ggcaaaaaaa ccttcattct gcatgatggc
     2101 cctccttatg cgaatggcag cattcatatt ggtcactcgg ttaacaagat tctgaaagac
     2161 attatcgtga agtccaaagg gctttccggt tatgactcgc cgtatgtgcc tggctgggac
     2221 tgccacggtc tgccgatcga gctgaaagta gagcaagaat acggtaagcc ggatgagaaa
     2281 ttcaccgccg ccgagttccg cgccaagtgc cgcgaatacg cggcgaccca ggttgacggt
     2341 caacgcaaag actttatccg tctgggcgtg ctgggcgact ggtcgcaccc gtacctgacc
     2401 atggacttca aaactgaagc caatatcatc cgcgcgctgg gcaaaatcat cggcaatggt
     2461 cacctgcaca aaggcgcgaa gccggtgcac tggtgcgtag actgccgttc tgcactggca
     2521 gaagcggaag ttgagtatta cgacaaaact tctccgtcca ttgacgtcgc tttccaggcg
     2581 gtcgatcagg atgcgctgaa agcgaaattt ggcgtaagca acgttaacgg cccaatctcg
     2641 ctggtgatct ggaccactac gccgtggact ctgcctgcga accgcgcaat ctctattgca
     2701 cctgatttcg actatgcgct ggtgcagatc gacggtcagg ccgtgattct ggcgaaagat
     2761 ctggttgaaa gcgtaatgca gcgtatcggc gtgaccgatt acaccattct cggcacggta
     2821 aaaggtgcgg agcttgagct gctgcgcttt gcccatccgt ttatgggctt cgacgtcccg
     2881 gcaatcctcg gcgatcacgt taccctggat gccggtaccg gtgccgttca caccgcgcct
     2941 ggccacggcc cggacgacta tgtgatcggt cagaaatacg gcctggaaac cgctaacccg
     3001 gttggcccgg acggcactta tctgccgggc acttatccga cgctggatgg cgtgaacgtc
     3061 ttcaaagcga acgacatcgt cgttgcgctg ctgcaggaaa aaggcgcgct gctgcacgtt
     3121 gagaaaatgc agcacagcta tccgtgctgc tggcgtcaca aaacgccgat catcttccgc
     3181 gcaacgccgc agtggttcgt cagtatggat cagaaaggtc tgcgcgcgca gtctctgaaa
     3241 gagatcaaag gtgtgcagtg gatcccggac tggggccagg cgcgtatcga gtcgatggtc
     3301 gctaaccgtc ctgactggtg tatctcccgt cagcgtacct ggggcgtacc gatgtctctg
     3361 ttcgtgcaca aagacacgga agagctgcat ccgcgtaccc tcgaactgat ggaagaagtg
     3421 gctaaacgcg ttgaagttga tggcatccag gcgtggtggg atcttgatgc gaaagagatc
     3481 ctcggcgatg aagctgatca gtatgtgaaa gtgccggata cgctggatgt atggtttgac
     3541 tccggctcta ctcactcttc tgttgttgac gtgcgcccgg aatttgccgg tcacgctgcg
     3601 gacatgtatc tggaaggttc tgaccaacac cgtggctggt tcatgtcttc tctgatgatc
     3661 tccaccgcga tgaagggcaa agcgccgtat cgtcaggtgc tgacccacgg ctttaccgta
     3721 gatggtcagg gccgcaagat gtctaaatcc atcggcaaca ccgtgtcgcc gcaggatgtg
     3781 atgaacaaac tgggcgcgga tattctgcgt ctgtgggtgg catcaaccga ctacaccggt
     3841 gaaatggccg tttctgacga gatcctgaaa cgtgctgccg acagctatcg tcgtatccgt
     3901 aacaccgcgc gcttcctgct ggcaaacctg aacggttttg atccggcaaa agatatggtg
     3961 aaaccggaag agatggtagt actggatcgc tgggccgtag gttgtgcgaa agcggcacag
     4021 gaagacatcc tcaaggcata cgaagcatac gatttccacg aagtggtaca gcgtctgatg
     4081 cgcttctgct ccgttgagat gggttccttc tacctcgaca tcatcaaaga ccgtcagtac
     4141 accgccaaag cggacagcgt ggcgcgtcgt agctgccaga ctgcgctgta tcacatcgca
     4201 gaagcgctgg ttcgctggat ggcaccaatc ctctccttca ccgctgatga agtgtggggc
     4261 tacctgccgg gcgaacgtga aaaatacgtc ttcaccggcg agtggtacga aggtctgttt
     4321 ggtctggcag acagtgaagc aatgaacgat gcgttctggg acgagctgtt gaaagtgcgt
     4381 ggcgaagtga acaaagtcat tgagcaggcg cgtgccgaca agaaagtggg tggctcgctg
     4441 gaagcggcag taaccttgta tacagaaccg gaactggcgg cgaaactgac cgcattgggc
     4501 gatgaattac gatttgtcct gttgacctcc ggcgctaccg ttgcagacta taacgacgca
     4561 cctgctgatg ctcagcagag cgaagtgctc aaagggctga aagtcgcgtt gagtaaagcc
     4621 gaaggtgaga agtgcccacg ctgctggcac tacacccagg atgtcggcaa ggtggcggaa
     4681 cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtga aaaacgtaag
     4741 tttgcctgat gagtcaatcg atctgttcaa cagggctacg ctggctgtgg ctggtggtag
     4801 tcgtgctgat tatcgatctg ggcagcaaat acctgatcct ccagaacttt gctctggggg
     4861 atacggtccc gctgttcccg tcgcttaatc tgcattatgc gcgtaactat ggcgcggcgt
     4921 ttagtttcct tgccgatagc ggcggctggc agcgttggtt ctttgccggt attgcgattg
     4981 gtattagcgt taccctggtt gtgatgatgt atcgctcgaa ggccacgcag aagctaaaca
     5041 atatcgccta cgcgctgatt attggcggcg cgctgggcaa cctgttcgac cgcctgtggc
     5101 acggcttcgt tgtcgatatg atcgacttct acgtcggcga ctggcacttc gccaccttca
     5161 acctcgccga tactgccatc tgtgttggtg cggcactgat tgtgctggaa ggttttttgc
     5221 cttctaaagc gaaaaaacaa taataaaccc tgccggataa ggcctacaga ttgctgcgaa
     5281 atcgtaggcc gcatccggca aaaatcctca aaatataaga gcaaacctgc atgtctgaat
     5341 ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca
     5401 ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggc gatgcttctc
     5461 tttccgaagg gctggagcaa cacctgctgg ggctgaaagt gggcgataaa accactttct
     5521 cgctggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc
     5581 gccgtgagtt tatggatgca ggcgagccag aaattggcgc aataatgctt tttaccgcaa
     5641 tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgtcg
     5701 atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg
     5761 atccggcact ggaggcgtaa catgcagatc ctgttggcca acccacgtgg tttttgtgcc
     5821 ggggtagacc gcgctatcag cattgttgaa aacgcgcttg ccatttacgg cgcaccgata
     5881 tatgtccgtc acgaagtggt gcataaccgc tacgtggtcg atagcctgcg cgagcgtgga
     5941 gctatcttta ttgagcagat cagcgaagtg ccggacggcg cgatcctgat cttctccgca
     6001 catggtgttt ctcaggcggt acgtaacgaa gcgaaaagcc gtgatttgac ggtattcgac
     6061 gccacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag ccgtcgtggc
     6121 gaagagtcta ttctcatcgg tcacgccggg cacccggaag tggaagggac gatggggcag
     6181 tacagcaacc ctgaaggggg aatgtatctg gtcgaatcgc ctgacgatgt gtggaaactg
     6241 acggtcaaaa acgaagagaa gctctccttt atgacccaaa ccacgctgtc ggtagatgac
     6301 acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa
     6361 gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag
     6421 gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag
     6481 ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga tatccaggaa
     6541 gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt
     6601 ctggtgcaga atgtggtggc acgtttgcag cagctgggtg gtggtgaagc cattccgctg
     6661 gaaggccgtg aagaaaatat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt
     6721 gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttatcggca
     6781 ttttttatgg agaaaacatg cgcttaccta tcttcctcga tactgacccc ggcattgacg
     6841 atgccgtcgc cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca
     6901 ccgtcgcggg taatgtctcg gttgagaaaa ctacccgtaa cgccttgcaa ctgctgcatt
     6961 tctggaatgc ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc
     7021 gtgatgcagc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca
     7081 accgaaagcc actcgggata ccggcgtttc tggcgattcg ggatgccctg atgcgtgcac
     7141 cagagcccgt taccctggtg gccatcggcc cgttaaccaa tattgcgctg ttactttcac
     7201 aatgcccgga atgtaaaccg tatattcgcc gtctggtgat catgggtggt tctgccggac
     7261 gcggcaactg tacgccaaac gccgagttta atattgctgc cgatccagaa gctgctgcct
     7321 gtgtcttccg cagtggtatt gaaatcgtca tgtgcggttt ggatgtcacc aatcaggcaa
     7381 tattaactcc tgactatctc tctacactgc cgcagttaaa ccgtaccggg aaaatgcttc
     7441 acgccctgtt tagccactac cgtagcggca gtatgcaaag cggcttgcga atgcacgatc
     7501 tctgcgccat cgcctggctg gtgcgcccgg acctgttcac tctcaaaccc tgttttgtgg
     7561 cagtggaaac tcaaggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc
     7621 tgggcaaacc agccaatgta caggtggcat tggatctgaa tgtaaaaggc ttccaacagt
     7681 gggtggctga ggtgctggct ctggtgccgt aacctgtcac atgttatcgg catgcagtca
     7741 ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc
     7801 agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat
     7861 taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg
     7921 gggcgtatgg gccgccagtt gattcaggcg gcactggcat tagaaggcgt gcagctgggc
     7981 gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt
     8041 gcagggaaaa caggcgttac cgtgcaaagc agtcttgatg cgataaaaga tgattttgat
     8101 gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag
     8161 catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggaaa acaggcaatt
     8221 cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac
     8281 gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatattgaa
     8341 attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg
     8401 ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt
     8461 gaaggccata ccggtgaacg tgtgcctggt accattggtt ttgccaccgt gcgtgcaggt
     8521 gacatcgttg gcgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc
     8581 cataaggcgt ccagccgcat gacatttgct aacggtgcgg taagatcggc tttgtggttg
     8641 agtggtaagg aaaatggtct ttttgatatg cgagatgtgc ttgat
//
LOCUS       DBFIFS010000181         8672 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.181, whole genome shotgun sequence.
ACCESSION   DBFIFS010000181 DBFIFS010000000
VERSION     DBFIFS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.181"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(362..1207)
                     /gene="sseA"
                     /locus_tag="N7A07_003710"
     CDS             complement(362..1207)
                     /gene="sseA"
                     /locus_tag="N7A07_003710"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU2478297.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            1414..6375
                     /gene="yfhM"
                     /locus_tag="N7A07_003711"
     CDS             1414..6375
                     /gene="yfhM"
                     /locus_tag="N7A07_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU2478298.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            6376..>8672
                     /gene="pbpC"
                     /locus_tag="N7A07_003712"
     CDS             6376..>8672
                     /gene="pbpC"
                     /locus_tag="N7A07_003712"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU2478299.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASV"
BASE COUNT         2039 a         2202 c         2442 g         1989 t
ORIGIN      
        1 tcggttaaca gcgcaccaga aacaataagt acgatacaga agcaccgtat cttcattgtg
       61 cggtccatgc gaagaccgca tcacgacagc accactggcg atattgccgc gatagcaagc
      121 actgtggggt gcctgtattt acggtcatgg aacattcttt cctaaagcca gcgcccgttt
      181 gcgagacggc gaaaagggcc attaagaata atactctcat gtttgaataa ttactctgtt
      241 tattatcttg acacagccag gcataaaaga gagtatttca acactgtggg gtgcctgcgt
      301 tgctccggca acacaagtca acctgctgga aaggccgcga accagaccag caataaaaag
      361 cttatttcaa tggctcaacc ggtaaatctg cccgcgcgcc ccattcactc catgcgccgt
      421 cgtacagttt cacgtttggc acatccagcg tcgcgagtgc caacaaaacc acggctgctg
      481 ttacaccaga gccgcagctg acgataattg gtttgtcata gctgacgccg cgaccaaaaa
      541 atatcgcatc cagttcatcg gtcgttttta gttcgccttc gcgcaccagt tccgtccacg
      601 gaacgttcaa tgcaccggga atatgtccgc gacgtaaacc tgggcgaggt tcatcaactt
      661 ctgcgttaaa acgtgcagcc gggcgggcat caataatttg cgccgtattt tcatggcttg
      721 ccaataacac atcggttact ttcaccactg cttcaggatt aaacgcggcg ttaaactctc
      781 cttccggcag ctctactgca ccttcttcta acaacagatc atcgcgctgc cagcctgcaa
      841 gtccaccccc cagaatcgac actttctcta caccaaaggt gcgcagcatc caccatgctc
      901 gtggggctga gaaaagatta ccttcgtcat agacaatcag gtgcttatcc tggttaacgc
      961 ctaattcacg catcgccacg gcgaacgttt ccgggcgcgg cagcatgtgc ggaagcgggg
     1021 aagtgtgatc agaaagcgct tcgatatcaa aaaacactgc gccgggaata tgtccattca
     1081 gatactcctg agcaacgtta cgatcttcct gtccaggcga cgccatgcgg gcatcgataa
     1141 tctgaatttc cgggtcatca atatgttcgg cgagccagtc ggctcctaca aaccatgtcg
     1201 tggacatagg catctccatt tttgccgggg ttaggctaaa ttgtcggatg tttagtgaaa
     1261 agcgacaagc aaaagaggct gatttggctg tctgagccac aattctcact aaaattcgga
     1321 ttgtatgggc gtgatactga attacataac gtaaattctc acgcataata attattcgac
     1381 gttgctaacg ggccattgtg gcagggatga aaaatgaaaa agttacgcgt agccgcctgc
     1441 atgctaatgc tggcgctggc agggtgcgac aacaacgata acgcgccaac agcggtgaaa
     1501 aaagatgcgc cttctgaagt tactaaagcg gcctcttcag aaaacgcgag ttcagcaaaa
     1561 ctctccgcgc cagagcgaca aaaactggcc caacagagtg ccggtaaggc gctgacattg
     1621 ctggatctct ctgaagtcca acttgatggt gcagccacgc tggtgctgac gttctccatc
     1681 cctctcgacc cggatcagga tttctcacgc gttattcatg tcgtcgataa aaaaagcggc
     1741 aaagtggatg gtgcctggga gctatcagat aatcttaaag agctgcgttt acgccacctc
     1801 gaaccgaaac gtgatttgat cgttacaatt ggcaaggagg tcaaagcact caacaacgca
     1861 accttcagta aagattacga aaaaactata actacccgcg acatccaacc cagtgtcggt
     1921 tttgccagcc gtggttcgct gctgcctggt aaagtcgttg aagggctgcc ggtaatggcg
     1981 ctcaacgtta ataatgtcga tgttaacttc ttccgcgtta agccagaatc tctgccagca
     2041 ttcattagcc aatgggaata ccgcaattcg ctggcgaact ggcagtcaga caaactgctg
     2101 cagatggcgg atctggtcta caccggacgg tttgatctca atcctgcgcg taacacccgt
     2161 gaaaaattat tgctgccgct gggcgatatc aaaccgcttc agcaggcggg cgtgtatctg
     2221 gctgtgatga atcaggctgg acgttacgat tacagtaatc ccgcgacgct gtttacgtta
     2281 agtgatatcg gcgtttcagc tcaccgttat cacaatcgtc tggatatctt tacccaaagt
     2341 ctggaaaacg gcgcggccca gcaaggaatt gaagtctctt tattaaatga gaaagggcag
     2401 actctgactc aggcaaccag tgacgctcag gggcatgtgc agctggaaaa tgataaaaac
     2461 gccgcattat tgctggcacg taaagacggt cagacaacgc tactcgattt aaaacttccg
     2521 gcgctggact tagcagaatt taacattgct ggcgcgccag gctatagcaa acagtttttc
     2581 atgtttggcc cgcgcgatct ttatcgcccg ggtgaaacgg taatcctcaa tggtttgctg
     2641 cgtgatgcag acggtaaagc gttgcccaat caacccatca agttagacgt gattaaaccc
     2701 gatgggcagg tactcaggag cgtcgttagt cagccggaaa atggcctcta ccactttacc
     2761 tggccactcg atagcaatgc ggcaaccggt atgtggcata ttcgcgctaa cacgggcgat
     2821 aaccagtatc ggatgtggga tttccacgtc gaagatttta tgccagagcg tatggcgctg
     2881 aatctgaccg gtgagaaaac cccgcttacg ccgaatgatg aagtgaaatt ctccgtggtg
     2941 ggatactacc tgtacggtgc gcctgctaat ggtaatactt tgcaagggca acttttcctg
     3001 cgcccactgc gtgaggctgt gtcagcctta cctggttttg aattcggcga tatagctgcc
     3061 gaaaaccttt cccgcacgct ggatgaagtt cagttgacgc tggatgataa agggcgcggc
     3121 gaagtttcta cagaaagcca gtggaaggaa acgcattccc cattgcaggt tattttccag
     3181 ggcagtttgc tggaatcggg cggtcgcccg gtgacgcgcc gcgctgagca ggctatctgg
     3241 cctgccgatg cattgccggg gatccgtccg cagttcgcct cgaaatcggt ttacgattat
     3301 cgcactgaca gcacggtgaa acagcccatt gttgatgaag gcagtaacgc cgcttttgac
     3361 atcgtttata gcgatgcgca aggcgtgaaa aaagccgtgt cgggcttgca ggtgcgcctg
     3421 attcgcgaac gccgcgatta ctactggaac tggtcagaag atgaaggctg gcaatcacag
     3481 tttgatcaaa aagatctgat tgaaaatgaa caaactctgg atctgaaagc ggacgaaacc
     3541 ggcaaggtca gtttcccggt agagtggggc gcttatcgtc tggaagtcaa agcgccgaat
     3601 gaagcggtca gtagtgttcg tttttgggct ggctatagct ggcaggataa tagcgacggt
     3661 agcggcgcag tgcgacccga ccgtgtcacg ctgaaactgg ataaagccgg ttatcgccct
     3721 ggcgatacca ttaagttgca tatcgccgcg ccaacggcgg gtaaaggtta tgcgatggtc
     3781 gagtccagtg aagggccgct gtggtggcaa gagattgatg ttccggctca agggctggat
     3841 ctgacgattc cggtcgataa aacctggaat cgtcatgatc tttatttgag tacgctggtg
     3901 gtgcgtcctg gcgataaatc tcgctccgcg acgccaaaac gcgcggttgg tgtgttgcat
     3961 ctgccgcttg gtgatgaaaa ccgtcgcctc gatctggcgc tggaaacacc agcaaaaatg
     4021 cgtcccaatc aaccattaac cgtgaaaatt aaagccagca ctaaaaatgg cgagaagcct
     4081 aaacaggtga atgtgctggt gtctgccgtt gatagtggtg tgctgaatat tactgactac
     4141 gtcacgccag atccgtggca ggcgttcttt ggtcagaaac gctatggcgc agacatttac
     4201 gatatttacg gtcaggttat tgaaggtcag gggcgtctgg cagctctgcg tttcggtggc
     4261 gatggtgatg agctgaaacg tggtggtaaa ccgccggtca atcacgtcaa tattgtcgcg
     4321 cagcaggcgc tgccggtaac gctcaacgaa cagggcgaag gctcggttac actgccgatt
     4381 ggcgatttta acggtgaatt gcgcgtcatg gcgcaagcct ggacggcaga tgaattcggt
     4441 agcaacgaaa gcaaagtgat agttgccgca ccggtgattg ctgaactgaa catgccgcgc
     4501 tttatggcga gtggcgataa ctcgcgtctg acgctggata tcactaatct taccgataaa
     4561 ccgcaaaaac tgaacgttgc cctgaccgcc agtggtttgc ttgaactggt cagcgattca
     4621 cccgcagccg ttgaattagc gccaggtgtg cgtactacgc tgtttatccc ggtgcgagca
     4681 ttgccgggtt atggcgatgg agaaattcag gccaccatta gcgggttagc gttaccgggt
     4741 gaaaccgttg ccgatcagca taagcagtgg aaaatcggcg tccgtccggc gttcccggca
     4801 caaacggtta attacggtac ggcgttacag cctggtgaga catgggcgat tccggcggat
     4861 ggattgcaaa acttctcgcc tgttacgctg gaagggcaat tgttgttgag cggcaaacca
     4921 ccgctgaaca tcgcacgtta tatcaaagag ttaaaagcgt atccgtacgg ctgtcttgag
     4981 caaaccgcca gcggcctgtt cccgtcactt tataccaacg cagcccaact gcaggcgttg
     5041 ggcatcaaag gcgacagtga tgagaaacgc cgtgcatcgg tcgatatcgg catttcccgt
     5101 ttgctgcaaa tgcaacgtga taacggcggc tttgcgctgt gggataaaaa cggtgacgaa
     5161 gagtactggc tgacggctta cgtgatggat ttcctggtcc gcgcaggcga acagggttac
     5221 agcgtgccga cagacgccat taaccggggt aatgagcgtc tgctgcgcta tttacaagat
     5281 ccgggcatga tgtcgatccc gtacgcggat aatctcaaag ccagtaaatt cgccgtacag
     5341 tcttacgctg cgctggtgtt ggcccgtcag caaaaggctc cgctgggtgc gctgcgtgaa
     5401 atctgggagc atcgtgcaga tgccgcttct ggtttaccgc tgctgcaact tggcgttgcg
     5461 ctgaaaacca tgggtgatgc gacgcgtgat gaagaagcga ttgcgctggc gctaaaaacg
     5521 ccgcgtaata gtgatgagcg gatatggctg ggtgattacg gtagtccact gcgcgacaac
     5581 gcgttgatgc tctccttgct ggaagagaac aaactgctac ccgatgagca gtactccctg
     5641 ctgaacacac tttcgcagca ggcgtttggt gaacgctggc tatcgacgca ggaaagtaac
     5701 gcgttgttcc tggctgcccg tacgattcag gatttaccag gtaaatggca ggcgcaaacc
     5761 tctttctcag ctgagccgct gacaggcgag aaagcgcaaa acagcaatct gaatagcgat
     5821 caacttgcca ccttgcaggt gaccaacagt ggcgagcagc cgttatggct gcgtgtggat
     5881 gccagcggtt atccgcaatc cgcaccttta ccggcgaaca atgtgctgca aattgagcgt
     5941 catattcttg gtactgatgg taagagcaaa tcgctggact cgttacgtag cggcgatctg
     6001 gtgctggtct ggttgcaggt aaaagctagt aacagcgtgc cggatgcgtt agtcgtggat
     6061 ctgctgcctg cgggtctgga actggaaaac cagaatctgg cgaacggtag cgccagcctg
     6121 gagcaaagtg gtggcgaagt gcagaactta ctgaaccaga tgcagcaggc gagtattaag
     6181 cacattgagt tccgtgacga tcgctttgtg gcggcggttg ccgttgatga ataccaaccg
     6241 gtaacgctgg tgtatctggc gcgggcggtg acgccgggaa cgtatcaggt accgcaaccg
     6301 atggtggaat caatgtatgt tccccaatgg cgggcgaccg gcgcggctga agatttgctg
     6361 attgtcagac cgtaaatgcc tcgcttgtta accaaacgcg gctgctggat aacgctggca
     6421 gctgcgccat ttctcctttt tcttgcagcg tggggagcag ataaactctg gcctctaccg
     6481 ctgcatgaag tcaatcccgc acgagtggtc gtggcgcagg atggtacgcc gctctggcgc
     6541 ttcgccgatg ctgacggcat ctggcgttat cctgtaacaa tcgaagatgt ttctccacgt
     6601 taccttgaag cgctgatcaa ttatgaagat cgctggttct ggaagcatcc tggggtgaat
     6661 ccattctcgg tggcgcgcgc agcatggcaa gatctcactt cgggacgggt tatttccggt
     6721 ggcagcacgc tcactatgca ggttgctcgt ctgcttgatc ctcaccccaa aacatttggc
     6781 ggcaaaattc gccagctctg gcgcgcgttg caactggaat ggcatctgtc taagcgtgaa
     6841 attctgacct tgtatcttaa ccgcgctccg tttggcggta cgttgcaggg gatcggtgcg
     6901 gcaagttggg cttatctcgg aaagtcgcct gcgaatttaa gctattccga ggcggcaatg
     6961 ctggcggttt tgccgcaagc gcccagccgt cttcgcccgg atcgttggcc ggagcgtgcc
     7021 gaagtcgcgc gtaataaagt gctcgaacgg atggccgtgc aaggtgtgtg gtcccgtgag
     7081 caggtaaaag agtcaaggga agaacccatc tggctggcac cccgacaaat gccgcaactg
     7141 gcaccgctgt tttcgcgcat gatgctcggt aaaagcaaaa gcgacaaaat cgttactacg
     7201 ctggatgccg gtcttcaacg acgtctggaa gaactggcgc aaaactggaa aggacgatta
     7261 ccaccgcgca gctcactggc gatgatcgtg gttgatcata ccgatatgcg tgttcgcggc
     7321 tgggtgggtt cggttgatct caacgatgat tcacgctttg gtcatgttga tatggtcaat
     7381 gcgatccgat cgccaggatc ggtgctcaaa ccgtttgttt acggtctggc gctggatgaa
     7441 ggattgatcc acccggcatc actgctgcaa gacgtccccc ggcgtaccgg tgattaccga
     7501 cctggcaact ttgacagcgg ttttcatggc ccgatcagca tgagcgaggc gctggtgcgc
     7561 tcgctgaatt tacctgctgt gcaggtgctg gaagcctatg gaccgaaacg gtttgcggca
     7621 aagttacgca atgttggatt gccgttatat ttgcccaacg gtgctgcgcc gaatctttcg
     7681 ctcattctcg gcggcgctgg tgcgaaactg gaagatatgg cggcagcgta taccgcgttt
     7741 gctcgccacg gcaaggcagg caaattgcgc ttacagcctg acgatccgct gcttgaacgg
     7801 cctttaatgt cgtcgggagc ggcgtggatc attcgccgga ttatggctga tgaagcgcta
     7861 cccttgccgg atggtgcctt gccgcgcgtc gccccactgg catggaaaac gggcaccagc
     7921 tatggctatc gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg
     7981 actggcagac cggacggcac gcccgttgtt ggtcagtttg gttttgccag cgccgtacca
     8041 ttgttgaatc aggtcaataa tatcttactg tcgcgcagtg ctaatctgcc ggaagacccg
     8101 cgcccgaact cagtcactcg tggcgttatt tgctggccgg gcggacagtc tttgccggaa
     8161 ggtgacggta actgtcgccg ccgcctggca acctggctgc tggacgggag tcagccgcca
     8221 actctattac tgccggagca ggaaggcatt aatggcattc gtttccccat ctggctggat
     8281 gaaaatggca aacgtgttgc cgctgattgc ccgcaagcgc gacaagaaat gataaatgtc
     8341 tggccgctac cgctggaacc ctggttgccc gcatcagaac gtcgcgctgt gcgcttacca
     8401 ccggcctcga caatttgccc gccatacggt cacgatgccc agctcccgct gcagctgaca
     8461 ggcgttcgcg atggcgagat tattaaacgt ttaccgggcg cagcggaagc gactttgccg
     8521 ttgcaatcaa gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact
     8581 gaacgcgggc gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg
     8641 atggatgacg tagggcaaat cgcgtcagta ca
//
LOCUS       DBFIFS010000182         8660 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.182, whole genome shotgun sequence.
ACCESSION   DBFIFS010000182 DBFIFS010000000
VERSION     DBFIFS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.182"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(49..342)
                     /gene="cas2e"
                     /locus_tag="N7A07_003713"
     CDS             complement(49..342)
                     /gene="cas2e"
                     /locus_tag="N7A07_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU2478300.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(339..1262)
                     /gene="cas1e"
                     /locus_tag="N7A07_003714"
     CDS             complement(339..1262)
                     /gene="cas1e"
                     /locus_tag="N7A07_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU2478301.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(1259..1909)
                     /gene="cas6e"
                     /locus_tag="N7A07_003715"
     CDS             complement(1259..1909)
                     /gene="cas6e"
                     /locus_tag="N7A07_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU2478302.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(1891..2637)
                     /gene="cas5e"
                     /locus_tag="N7A07_003716"
     CDS             complement(1891..2637)
                     /gene="cas5e"
                     /locus_tag="N7A07_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU2478303.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(2648..3703)
                     /gene="cas7e"
                     /locus_tag="N7A07_003717"
     CDS             complement(2648..3703)
                     /gene="cas7e"
                     /locus_tag="N7A07_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU2478304.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3715..4251)
                     /gene="casB"
                     /locus_tag="N7A07_003718"
                     /gene_synonym="cse2"
     CDS             complement(3715..4251)
                     /gene="casB"
                     /locus_tag="N7A07_003718"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU2478305.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(4248..5810)
                     /gene="casA"
                     /locus_tag="N7A07_003719"
                     /gene_synonym="cse1"
     CDS             complement(4248..5810)
                     /gene="casA"
                     /locus_tag="N7A07_003719"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU2478306.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(5908..8607)
                     /locus_tag="N7A07_003720"
     CDS             complement(5908..8607)
                     /locus_tag="N7A07_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGU2478307.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAP
                     ELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
                     GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKE
                     DGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT         2093 a         2387 c         2043 g         2137 t
ORIGIN      
        1 tctaccaact aaattcaaac atttccttat ttttaaagaa cacataacct attgattatc
       61 aacaggaaga aaagaaacca aacgtaaccc atccaaatcc accggaatac gtctgttttc
      121 tccccaggtc tgaaattcaa aacccgactc ggtattggtc gcccaggcca tcaccacatt
      181 tccgcaacca gccagttggg taatttgctg ccagatcatc tcccgaatac gttttgatgt
      241 atcaccaaca tacacaccgg cacgcacttc cagtagccag attgcgagcc gtccacgtaa
      301 gcgcggaggg acattttctg taacaaccac gaccatgctc atccgccgcg cccccggtga
      361 ccactatcac ccagcgtttc aggttcaggg atggcaggcg gtaacatatc cggcgcgggt
      421 tgtggtggtt caatttcacc tgcagcaagg actttctcaa ttaacggtat taatttgccc
      481 gttaacttag tgctacggaa aatatcgcga caggctaatc tgacttcttt atcaggttct
      541 gcgggttgcc tcgctgctat ttcaaatgcc tttggcacaa ccgaatcaaa tttaatgata
      601 tcggctatgt cataaacaaa tgaaagcggt ttgccactat gaataaatcc aatagcgggc
      661 gcatatcccg cggctaatac tgccgcttca gaaataccgt acagacatga tgtggcagca
      721 ctgatgcagc gattcacaac atcgcctttt tcccagtctt taggatcgta tttgcgacca
      781 ttccatttca caccatattg tttcgccagt aatgcatagg tctggcgaac gcgggatccc
      841 tcaattcccc gtagctgatc cactgaacgg cgagctggcg gtggctcacg aaaacgtaat
      901 tcatacattt tgcgcaccac cttcaggcgt agatcttccg ttaaagccag ctttgcctgg
      961 tagagtaatt tatctgcccg cgcccctccg ggttgtccgg aagagtaaac gcgaacgccc
     1021 gcttcaccga cccagaccag cagtgttccc accgtggcgg ccagatgcac cgccgcgtgg
     1081 gaaactctcg ttcccggttc gagcataatg caggcgaccg atcccaccgg aatgtgcgtg
     1141 cggatcccgg ttttgtcgat cagcacgaaa gcgccgtcca gtacgtcgat ttgaccgtac
     1201 tggaggaaga tcatagaggt gcgatctttt aacgggatcg gactcagtgg tacaaacgtc
     1261 acacctctgc tccgggtttg atcagcatca gaccacagcc gaacgcgcgg cttttgccat
     1321 acccctgact gagacgttgc agaaataacc cgggatcggt gaccgtaagc atccccgtat
     1381 aatccacgct actgaactgg atcagttgcc gggagttttc ccgccgcagt tgctgttgtc
     1441 ggtaggcatc aaccgaggta tccagcagtg taaaaccgct tctctccccc tgcgctgcca
     1501 gccagtccag cgccgcctgt tgttgatgca accagacatc acttccttcc gcctgccccc
     1561 tcacctgccg tttcgcctcc atcagcagat cgtggcgctt gcccgcttta cagattgttg
     1621 gattcgcccg caggttgaaa caaagttgtt gtccggtacg cagctcgggc acaaatgacc
     1681 ggcattcgat ggtgaacgtt tcactttccg ccgggcgttc ctgcgacagt acaaaaaagc
     1741 gaaacgcgcc ctggagttct tcccgacgat aaagaaattg cctttctttg ccgccaggga
     1801 agagatccca cagccactga tgcatcacat attccccgcg atccaccaaa tgcagcaact
     1861 gcgcaggcga aagctggccg gtatgtaagg ttattcttga gaggtacatg gttcctcctt
     1921 gctgagccac ggcccctgat tgatggtgcg ttccccaaac agccactgct gacgatttaa
     1981 aggaacatcc cggcgacgta atattttgct cgcgaccagg ccgtcgtgtt ccccttccca
     2041 ccagcattca tcctgaagtt tcgggagtga gactttcagt tcgcggaaac tatcctgata
     2101 ctgttggtat gcgttacgta agacatcaga cgcgttgcct tcgagcagta acggcgcaag
     2161 tggtaacgcc agcggatgac tttttcgccc cagataaagc ggaaaaaccg gatgacgtaa
     2221 accgtcctgc aactgttcaa ggctgtaagg cgcatcgggg gttgttgcca ccgccaccat
     2281 ccaccaggca tcggtgtagt agtcgcgccg ggagataatc gcgctcagaa gatcaggggc
     2341 gctcaactct tcgcgacggc tgaaataacg cgctttacgc acctcttttg gcatctggac
     2401 cgtgtgataa tcccgtgccc agcgcgggtt acggctggcg caaaccacca gtgaatagtg
     2461 gcggttaaac gcgtttaatc gttcggtatc atcacgccga atccctaccc cggcagccag
     2521 cagccccagc aatgctgagc gcgaaggcag ttcatgggta tgacgcactt cgccgggggc
     2581 atcgacgccc caggatgcca ttggcccatg aagctgaaaa atcaaatatt ggctcattag
     2641 ccgcccctta cgcgcagata aagtccagca cgtccttcat gcttccctgt ttgttcatca
     2701 cgtcaaagct tgcgcattcg gtcttctgtt catagaccgt attcatattt tcgcgaagcg
     2761 ttgtaatacg ctgcaccgcc acatctaact gccgggtgcc attaatgggt tcatagaaag
     2821 ccgccgccag agaacgtggt tgttcggtgc ctttttctgc cagcgcccag gaggcgtagg
     2881 cacggctggc aaagctgttc tgtttgccgg ttggggagac tttaagtgcg gcttccgtaa
     2941 aggcgcgcaa ggtctgatta gctaacgctt cgtcaccgcc gaggttttcg accagcagat
     3001 ctttatcgat gcagatatag gtgtagaaca gcgcagaacc gaatccggtt tcaccaagat
     3061 gcccggcacc ggcatcttca gaagcctggc gcaaatcatc aacggcggtg aaaaaatcat
     3121 cttcgacaat cgtttcactg acaccaaatg catgcgcgac ctggcaggcg gcttcaacat
     3181 taaattcggg tttattcgcc agcatacgac caaacatagc gatatctact gccatgcgat
     3241 ctttacgtaa caaagcgaga tcttcctctt ttggcgcgcg cttttcttcg gccagttgat
     3301 gggccagcgc ttttacggcg tcaaattctg ccgggctgat atggactaat tgttcagttt
     3361 cggcgttagt gagcggatct tttggttttt tgtcgttttt agctttccca agataatccg
     3421 caatttttgc cgcccattcg atggcttttt tctcttcgat gcctttctca atcaggatag
     3481 ttgccgcctc acgcgcaata cgcccactgc gaataccaat atggcccgcc agtgcctgtt
     3541 caaaaagtgc agaagtgcgc cacgcacgtt tcagactttg cgaggaaacg cgcagtcgcg
     3601 ttgctccacc caggaccacg gttttcggcg ctccggtgtc atcacggtta aggttggctg
     3661 cagggtaagc ggttaacaaa tgaagctgaa taaatgtcgt catgagatag tcctttattg
     3721 attttgttcg ttatcgacgt cgccagcctg gtaatattcc agcgcccagc ggatacggat
     3781 aaattcggtt ggccgctgat gacggcggtg atgattgagc agatcgtcgc tctcctggca
     3841 ccagcggaag acaccctctg ccagagagtc aagattaacg gaaccgttta ataatctgac
     3901 tacgcggcgt aactggcgca gtaattcatc cggtgttttt actgccgaca ggcgagtaaa
     3961 acgccccttt gacattaccg ccgccagctg cgcagcaaaa ggcagccgtt catcaatggc
     4021 tttaacatta gcgctaagtg cggctatcag cgccagtgcg gtaatacgcc attcaggttc
     4081 atcctgccac tttatttgtc tgttcttcag aaacaggcga aatccatccg tcagacaaac
     4141 atcattaacc gtcgtactac gccgcagact ggcacgttcg ccgcgtttct cctgcaattc
     4201 ctcatgccat ttgcgcagcg tggctttgtg ctcctctttt acaatactca ttcagcagcc
     4261 tcctgctttt tctccctggc ggctttagca ctttgcttct ccgccgatgt tgtaaaatat
     4321 ttcttgcgcg cggtcatgac gcgttccaaa tcaacgggct cataaggatt ggtgaataca
     4381 cgctcgtcaa aatcctgacg tgcgaataac caaatttcct tttgccattt gccgagtaat
     4441 tcatccgcat cctgaccttc ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt
     4501 ttgttccaga agtcgatatc cacaaaactg aaatcacccc ttgcaccttt tggatcggag
     4561 aaccatgctt ctttcaatgc actccgtaac agactcagaa tccgtgaagc cgtttgcgca
     4621 gccagccgca gcttcggtat ctggccttct tttttattga gcagcagcgg gaaatggtgt
     4681 tcgtaccaac agcgcgcttt catgttgtcg aaatcataac caaatcccca caggcccact
     4741 tttgcctgtt tcagactgct ggcattaaag agtttcacca ccagcgcggg aagttccgta
     4801 ttgttttctg acttacccgt ttcgataagg cctaaccagt cgcgccagat taaaccgccc
     4861 ggttgtggtt taacggagta aaactcaccg ccctctttaa gtggtacacg gtaaggcgtt
     4921 aagggatgct gccacatggc ataattcgca ccgtaatttt tggtagtcat caaactcaga
     4981 agcgcgtcac tctgctcacc gcaaatatcg cagttgccga ctgtcgtggt attaaaatca
     5041 atacgaatac gccgcggcat tccccagtac gcctggagtt tattgacctg atcatcggtt
     5101 accaccgcac cggccagttc gctggtacgc gtcgggccaa gccaggggaa aaccagatcg
     5161 tcaaattttt tgggtagcgg taagtcggct tcatcctgcg gcatcacgtt gagccacagt
     5221 ttgcgccaca agggggcttg ttgattgccc tgatactcct gcaattcaat cagagtcgtc
     5281 atcggcccac cgccgcgtaa accggtgcga tagcctttgc cacctgacgg cgcatttaac
     5341 tgtagggaga acagagctaa cgcagaacaa tgagagcata cgtgttcagt cacgccacgc
     5401 ttaataaagt ggtctttatt aaacttcgtt gtttgagcgc cgggaatctc aggcagtagc
     5461 gaagcgacct gaactttatc gcccatgagc acctcgaaat cctgcataaa tgaaggtgaa
     5521 tctgggccaa actggaaagg gtgttctaat gacagcaatg cttcccgtag cttttcagct
     5581 tccagcccgt cttcccagat atcatcccaa cgacgataat cttttggcgc gaaactgctt
     5641 tgtagtaacc ccagcaaaaa ctgccatgcc gccccctgga gatctgcccg cggcgcagcg
     5701 atatcgacaa cattttcatc cgccagatcg actggcgcca gcttgcctgt tgttccgtct
     5761 ttaaaacgaa cgggcaacca cggggttgtc agaagtgaaa acgagttcat atgtatcctc
     5821 tcgtaaaaaa gaccatccct ggtcggttgt ccatcattcc atccttttcg gtgtatcaac
     5881 tattcagaga gtcctgtcgc aggtatctca atcaaccttg ccaatcaatc cctccctggc
     5941 cgaatacccg caactttcgt catcagtgac taaaatcacg tttgccattt ccggatcttg
     6001 ccgctgttca atgcaccact gcctgaacgc ttccccttcc agtaaagaaa actcatcccg
     6061 atgttttttc caccagcttc gacgcactct gacaacgctc atttcccatg cgtgagcacc
     6121 ggtggcataa ggcttcacca caccggcaat acaggtagcc agccacaggg aaacagattc
     6181 ctcagccaga cgtgtcgaca gcttttccgg aaggtaatcg ttgatattgg cggcatagcc
     6241 aggcttgaag ttcaggacaa actttttagc cattgcgcga tcgcagtaat atttgcccac
     6301 ttgctcctgc tcgctgcggg caaatccttc cggcattacc acgtcctcac cgtagactga
     6361 ttcaataaga aggcgggctg cgtgtggcat ttgaatagcg ccttgctcac gcagtacacg
     6421 ctgcgtcagc cagattcgtc catgatcggg atagacatat gcactgttac gcatggcact
     6481 gccgaaccat tcgtcaccag gagcgtcgtc ccagacgggg gccagaatca gcaattcagg
     6541 aggggaacgc tcgtcttttc cgtcacgctt taactgacca ttaatatcgc ggatatgccg
     6601 ctgtaatcgc cccgctcgct gaatcagcaa atcaacaggg gccaggtcgg agatcatttc
     6661 gtccaggtca caatcaacgc tctgctctaa gacctgagta caaatgagga cttttccggc
     6721 acgctgtgaa ccgtcttctt taccaaagcg tgccagcgtc tccatttcaa ttcgctggcg
     6781 atcgctaaaa gcaaagcggc tatgaaagag tgaaaggctg gaagcgggaa tgacgccgcg
     6841 ggcaagcagc tgacgatgaa ccttaatagc gtcatcgaca gaattccgga tccaggcgat
     6901 gcattttccc tgacttaccg ccgattcgat acgcgcaata ctctcttgtt cactatgaag
     6961 ccaacccacg ctgacgctac gctcaacgtc tttgcgcgtc gctacccggt gtgagttcac
     7021 atcggatttc gtgacatgcg tcagccaggg gtaatcatcc ttttcaagga acggagcttc
     7081 ttgctggccc tctgtgccac gcgcaaaggc ggcgacgagt ttgtcgcgct gctgttggga
     7141 taacgtagca gaaagcaaaa tgacgctgtt tccgccacgc gcctgccgct cgatcagccc
     7201 ttcaagaatg cacgacatgt aagcatcaca ggcatggatc tcatcagcca gcaggatttt
     7261 gttactcaac cccagaagcc gcagattatt atgtttaaac ggcatcactg ccatcatcgc
     7321 ctgatccagc gtgccgacgc caatttcagc cagtagcgcc ttcttgttac tgttggcaaa
     7381 ccaggccgca catccctgac tgaatgtttg ttcatccggt tcttctgacc cgactaaatc
     7441 accggaccag agtgattcat tgaagcggtc cattaatgtg cgggcactgt gtgccagcac
     7501 caagctgggg cgggactctg gcgaatagaa agcaagccag gttttgacca gccgatcgta
     7561 catggcattg gccgttgcca ttgttggcag gccaaaaaac aaaccctgtg ctttcctcgc
     7621 agccatcaac ctgtgcgcca ggataagcgc cgcttctgtt ttacctgcgc cagtcacgtc
     7681 ttccagaata aataactgtg gccctggctg gctgatatcc agatccagta ccttttgctg
     7741 taatggtgtc gggtgctcaa taaaaggaaa cagcgtatta attccggtga aaggtgcggt
     7801 ttctgctttt ggaggaaaga cggttaaggc gttttgagcc tgaactaaag ttttctgcca
     7861 gtaatcttta atatccattg ggtgtgcgac gcgtggaaaa aatcgcgttg acgaacccgt
     7921 ccagtctgcg agtacgactg ttgcagagat ataccaggaa agttgtttta aaagttcaac
     7981 gccctcgtca tcatcccaga atgtgggaat ctctatgagc ggaaacagtg ccttgatttc
     8041 aaggagaaaa tctcgcgcgg cagctttgtc ttcaggcaga aaattatcca gctcatcaat
     8101 acggtcaggt ggtcgaccat gatgcccggt agttatggac atccacatct ctattacacg
     8161 tgtaagttta cgagaagaga gtgaagatga aggaagcaac tcctcacatt cacttaaata
     8221 ataattccac agccagtaac ccagcgttga atgagagatc ttttcgtaat tctttctgga
     8281 accttccgga atcttgagtt caggggccag gtaaagttgc tgaaaagagc gggcaaattt
     8341 tccaatatcg tgccagcaca gcaaccaagc gaaaaattga gccgcctgtt ccttgtcaga
     8401 aatccctaat tgacgaaagt aatcagccag cccgaagcaa tttcttttaa ccattaaata
     8461 gcccattgcg gccacatcca gcgaatgcca gcaaagaagg tgatagccgt cgccaccctc
     8521 tttctcgcca cgtcgggttt ttccccagaa atcaaagaaa gtcacaatat ttttatcctt
     8581 cagtaaactt aaaggatatt tacgcatatt ttaaaaatta tctgtgatat atatcagcca
     8641 ataaacattt atattatgaa
//
LOCUS       DBFIFS010000183         8626 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.183, whole genome shotgun sequence.
ACCESSION   DBFIFS010000183 DBFIFS010000000
VERSION     DBFIFS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.183"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..302
                     /locus_tag="N7A07_003721"
     CDS             <1..302
                     /locus_tag="N7A07_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU2478308.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..1369
                     /locus_tag="N7A07_003722"
     CDS             299..1369
                     /locus_tag="N7A07_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU2478309.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            1709..2527
                     /locus_tag="N7A07_003723"
     CDS             1709..2527
                     /locus_tag="N7A07_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2478310.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2618..3103
                     /locus_tag="N7A07_003724"
     CDS             2618..3103
                     /locus_tag="N7A07_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2478311.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            3119..3595
                     /locus_tag="N7A07_003725"
     CDS             3119..3595
                     /locus_tag="N7A07_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU2478312.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3658..3879
                     /locus_tag="N7A07_003726"
     CDS             3658..3879
                     /locus_tag="N7A07_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU2478313.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4042..4416
                     /locus_tag="N7A07_003727"
     CDS             4042..4416
                     /locus_tag="N7A07_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU2478314.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            4463..4837
                     /locus_tag="N7A07_003728"
     CDS             4463..4837
                     /locus_tag="N7A07_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU2478315.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            4834..5325
                     /locus_tag="N7A07_003729"
     CDS             4834..5325
                     /locus_tag="N7A07_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2478316.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            5337..5534
                     /locus_tag="N7A07_003730"
     CDS             5337..5534
                     /locus_tag="N7A07_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU2478317.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            5619..6461
                     /locus_tag="N7A07_003731"
     CDS             5619..6461
                     /locus_tag="N7A07_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU2478318.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6611..6698)
                     /locus_tag="N7A07_003732"
     tRNA            complement(6611..6698)
                     /locus_tag="N7A07_003732"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6662..6664),aa:Ser,seq:gga)
     gene            6932..7870
                     /gene="ghrA"
                     /locus_tag="N7A07_003733"
     CDS             6932..7870
                     /gene="ghrA"
                     /locus_tag="N7A07_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU2478319.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            7925..>8626
                     /gene="ycdX"
                     /locus_tag="N7A07_003734"
     CDS             7925..>8626
                     /gene="ycdX"
                     /locus_tag="N7A07_003734"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU2478320.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESR"
BASE COUNT         2109 a         2213 c         2258 g         2046 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcaggggc
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct accgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct gggatcatct ggggaataat caggtgctgt cggtaaacag
      541 attggtttac ttttcattgc tgaaaaaatt gttccaggca atgaacctct gccagatccc
      601 cggacgtgca ggagcactgc ttacgacgct tcttcgccat atatcgacaa tcaacgggtt
      661 tgacaatctg atggctcagc cgttgttgtc agatgagccc ctgacagcgc tgatggatca
      721 ttatcttgat actgatgctc tggcagacgg gctaccgctg tatgtgtcgc tgtaccccac
      781 agaagggggc atgcaggata ttattgactg cattcgtgct gaactgggtg tcggaaccac
      841 gaaaaacgcc gtttttcagc atatccagag cctgccccgc ggacagcaga aagaggctct
      901 gcttgcgtca gccgcgctgc ccctgctgtt ccgtccccgt gaggttcagg ggacaatgtt
      961 cggtgatggt ggtatgggag gatggcgaaa tatgcaggga aatacccctg tgacgcctct
     1021 ggtcgatgcc ggatgcaata tggtgattgt gacgcatctg agtgacggtt ctttatggga
     1081 tcgccaggct tttccggaca ccacaatcct tgagatccgt ccccggaaaa ggctgaaata
     1141 tgcaggtgat ggtggcaaca gcggcggtct gctcagtttt acatcggcac ataccgacgc
     1201 ctggcgtcag cagggctatg aagacacgat gctggcgatg gagcatatcc ggaaaccgct
     1261 ggcagcacgt caggcactga cccggtcaga ggcggtattg cagaaaagcc tggatataac
     1321 ggaagaggca gatttggcac tgagaaacgc gatggcccgg attaaataaa gacgccccaa
     1381 agaaacgcca ccgcctgtgg tggtgttttt cctgtctggt gttattgaaa atattttcag
     1441 atatatatta tctgggtgat ggttaagatg aatttattta attttggggg aagatacaaa
     1501 aacaaccagt gaacatattt ccggcgggtt atccgcaatg ctgaaggtca gttgcactgg
     1561 cgatgccgga atacagctta atacgtggtt cgccagtgaa gggcttctca gacaataaac
     1621 ctcattcatt tcacccatca ggccgcgtct tctccgggag acgcggcttt ttcatttata
     1681 tcgcttaatc attcataagg agcaaatcat gagattagcc agtcgttttg gttatgcgaa
     1741 ccagatacgt cgtgatcgtc cgctgacaca cgaagaactg atgcgccatg taccgagtat
     1801 ttttggggaa aaccgacata cctctcgcag tgaacactat gcgtacattc ccactatcac
     1861 tgtcctggaa aatctgcagc aggaaggctt tcagccgttc tttgcctgcc agacccgcgt
     1921 gcgcgaccag agccgccggg aatacacaaa acacatgctg cgtctgcggc gggccggaca
     1981 gataaccggt cagcatgtcc ctgaaattat tctgctcaac tcccatgacg gttcatccag
     2041 ctaccagatg ttacccggat attttcgtgc catttgtacc aatggactgg tctgcggtca
     2101 gtctctggga gaattgcgtg ttccacaccg gggaaatgtg gtggacagag tcattgaagg
     2161 ggcttacgag gtggtgggcg tttttgaccg gatagaggag aagcgtgatg ccatgcagtc
     2221 gctggtcctg ccgccaccgg cacgccaggc gctggcacag gcggcactga cttaccgtta
     2281 tggtgacgaa catcagcccg tcaccaccgc cgacattctg acaccacgac gccgggagga
     2341 ttacggtaag gacctgtgga gtgcttatca gaccatccag gagaatatgc tgaaaggcgg
     2401 gatttccggt cgcagtgcaa aaggaaaacg tatccatacc cgggccattc acagcattga
     2461 taccgacatt aagctcaatc gcgcattgtg ggtgatggca gaaacgctgc tggagagcat
     2521 gcgctgatgc cgtttcactg aaagtgcagt cctgactcac atctgttcct gccccggggc
     2581 ttcttccatt catttacttt ttataaggaa taacctcatg acaaccgttt cgcataattc
     2641 caccacacct tctgtttccg taaccactgc atcagggaat aacccacctc agctggttgc
     2701 cactcccgtc cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc
     2761 acagtgggtg accctggagc cccgtgtctt cggctggatg gaccgtcttt gcgaagacta
     2821 ctgcggtggt atctggaatc tgtacaccct gaacaacggc ggggcattta tggcacccga
     2881 accggatgac gatgatgacg aaacatgggt actgttcaat gccatgaacg gcaaccgcgc
     2941 tgaaatgagc ccggaagccg ccggtattgc tgcctgtctg atgacgtaca gtcatcatgc
     3001 ctgtcgtacg gagtgttatg ccatgacggt ccactattac cggctgcggg attacgccct
     3061 gcagcatccg gaatgcagcg ccattatgcg catcattgac tgaatggagg cagggacaat
     3121 gcagcagctt tcctttctgc ccggagagat gacgcccggc gagcgcagtc tcattctgcg
     3181 ggccctgcaa accctggacc gccatcttca tgaacccggc gtggccttca cctccacccg
     3241 tgcggcacgg gaatggctga ttctgaacat ggcgggactg gagcgggaag agttccgggt
     3301 gctgtatctg aacaaccaga atcagctgat tgccggtgaa accctcttca ccggcaccat
     3361 caaccgtacg gaagttcatc cccgggaagt gattaaacgc gccctgtacc acaatgccgc
     3421 tgccgtggtg ctggcgcaca atcacccgtc cggtgaagtc acacccagta aggcagaccg
     3481 gcttatcacc gaacgtctgg tacaggcact gggcctggtg gatatccggg tgccggacca
     3541 tctgatagtc ggtggcaacc aggttttctc ctttgccgaa catggtctgc tttaacccgt
     3601 cacaaccaca tcacacctgt tttcactttt atcttctgtc ttcagaggta tcacatcatg
     3661 agaatcatca cccgtggtga agccatgcgt attcaccaac agcatcctgc atcccgtctt
     3721 tttccgttct gtaccggtaa gtatcgctgg cacggcagtg ctgaagcgta tactggtcgt
     3781 gaagtgcagg atattcccgg cgttcttgcc gtgtttgcag aacgccgtaa ggacagtttt
     3841 ggcccgtatg tccggctgat gagtgtcacc ctgaactgat gttccgtcac ccgggaaacg
     3901 agtacgacca gatgacctca gacgccgctg tggatgaggc cattctcatc aatgcataca
     3961 tcttcaccga aaccggtcag cgcgccggct gacctgtcat cgttaaacca ttcttaacca
     4021 cttttcagag aggattttat cgtgtcagac aaactctccg ggataactca tcccgatgac
     4081 aatcacgacc gcccctggtg ggggcttccc tgcacagtga ggccctgttt tggcgctcgt
     4141 ctggtgcaag agggtaaccg cctgcattac cttgccgacc gcgccggtat cagaggccgc
     4201 ttcagcgacg cagatgcata ccatctggac caggcctttc cgctgctgat gaaacaactg
     4261 gaactcatgc tcaccagcgg tgaactgaat ccccgatatc agcataccgt cacgctgaat
     4321 gcaaaagggc tgacctgcga agccgacacc ctcggctcct gtggttacgt ttatctggct
     4381 gtttatccga caccagcacc cgcaaccacc tcataacccc gaagctctca cctttagcac
     4441 taccagttac agagaactta agatgaaaac attatccgac acacatgtac gggaggtatc
     4501 tcgctgcccg tctcccgtca ccatctggca gacactgctc atccgactgc tggaccagca
     4561 ttatggcctc acactgaatg acacaccgtt tgccgatgaa cgtgtgattg agcagcatat
     4621 tgaggcaggc atttcactgt gtgatgcagt taattttctc gtggaaaaat acgcgctggt
     4681 gcgtaccgac cagccgggat tcagcacctg tccccgctct cagttaataa acagtattga
     4741 tatcctccgg gctcgccggg cgaccggcct gatgacccgc gacaattaca gaacggtaaa
     4801 taacattacc ctgggtaagt atccggaggc gaaatgatga aactggccct gacactggaa
     4861 gccgacagcg ttaacgtaca ggcactgaac atggggcgca ttgtcgttga cgtcgatggt
     4921 attgagctcg ctgaactgat taacatggtc tgcgataacg gctactccct tcgtgttgtt
     4981 gatgaatctg accagacctc agcagaatgc acgccaccat ttgctaccct taccggcata
     5041 cgctgcagta ccgcacatat cacggaaacg gacaacgcct ggctgtactc gctgtcacac
     5101 cagaccagtg acttcggtga atcagaatgg attcatttta ccggtaacgg ctatctgttg
     5161 cgtaccgatg cgtggtcgta cccggttctg cggcttaaac gcctgggtct gtcaaaaacg
     5221 ttccgtcgtc tggtcgtcac actcatccgg cgttatggcg tcagtctcat tcatctggat
     5281 gccagcgctg aatgcctgcc gggtttaccc actttcgact ggtaaccagg aacaacatga
     5341 aatcattaac cacggaaaca gcgctggata ttctgattgc gtggctgcag gacaatatcg
     5401 actgcggatc gggaattatc tttgacaacg atgaggataa aacggattca gcagcactgt
     5461 tgccctgtat cgaacaggcc cgggaggatg tccgtaccct gcgtcatctg cagcttctgc
     5521 accagaaccg gtgaagctca ctcaccagac ttcattcctc tgacgccagc ctgcacaggg
     5581 ctagcgtttt catttatctg caaaaaggaa tatcgattat gtcagatatc acaatctccc
     5641 gtccggaagt ggtcaacggg catacggacg ttatctgctc aaccagcatt cgccacattc
     5701 tggctgtacg aaagagtaca ctgttgcaaa tcgacacact tatccgacaa ctggctgaaa
     5761 tctcggtgct gacggaaagc atcggaggta aaactgcccc ggactgggcc atgaaacagg
     5821 attttcgctg cggctgctgg ctgatggaga aacccgaaac agcaatgaaa gccattaccc
     5881 ggaatcttga tcgcgaaatc tggcgtgacc tgatgcaacg ttccgggatg ctgtcattaa
     5941 tggatacgca ggcccgtgat acatggtacc ggtcactgga gtacgataat tttccggaaa
     6001 tcagcgaagc gaacattctg agcacatttg aacaactgca ccagaataag gatgaggtgt
     6061 ttgagcgagg ggtgatcaac gtcttcagag ggctgaactg gaattacaaa accaattgcc
     6121 cctgtaaatt tggcagtaaa attatcgtca acaatctggt gagatgggac cggtggggat
     6181 ttcatcttat caccggacaa caggctgacc gactcgccga tctggaaaga atgttgcacc
     6241 tgttcagcgg taaaccaatc cccgacaacc ggggaaacat cacccttcgt ctggatgatc
     6301 acattcagtc tgttcagggt aaagagagct atgaagatga gatgttcagc atcagatact
     6361 ttaagaaggg ctccgcgcat atcacgttca gaaaaccaga actggttgac agactgaatg
     6421 atattatcgc aaaacactat cctgatatgc tggcagtata gctttgccag tatgctcaaa
     6481 gatcggaaat tgtccgataa gatatgatcg cggggcatat aaaccggttt cagatggcat
     6541 acccgatggc atactgtctg gaagggaaaa taaaaaagcg catgttaaac aataacatgc
     6601 gcttataatc tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact
     6661 ttccaggcgt gctccttcag ccactcggac acctcaccaa attgtcgttc ctgtcttgct
     6721 ggaacgggcg ctaatttagg gaaatcatga cctgaggtca acaaactttt ttaaaaaatc
     6781 gcgcgtttat tcaaacttca atcaatatgc agttttaata agcaaaatct gcttttttgg
     6841 ccaccgacca cggatttgtt atgctggtgg tctttgtaga tcataacgat aagtgcgaat
     6901 aaatttcgca caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt
     6961 cgatacccaa tggtggatag aggcactgcg caaagctatt cctcaggcaa gagtcagagc
     7021 atggaaaagc ggagataatg actctgctga ttatgcttta gcctggcatc ctcctgttga
     7081 aatgctggca gggcgcgatc ttaaagcggt gttcgcactc ggggccggtg ttgattctat
     7141 tttgagcaag ctacaggcac accctgaaat gctgaagcct tctgttccac tttttcgcct
     7201 ggaagatacc ggtatgggcg agcaaatgca ggaatatgct gtcagtcagg tgctgcattg
     7261 gtttcgacgt tttgacgatt atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc
     7321 tgaatatcat cgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggcagtaa
     7381 agttgctcag agtctgcaaa cctggcgctt tccgctgcgt tgctggagtc gaacccgtaa
     7441 atcgtggcct ggcgtgcaaa gctttgccgg atgggaagaa ctgtctgcat ttctgagcca
     7501 atgtcgggta ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca
     7561 acaattactc gaaaaattac cggatggcgc gtatctcctc aacctggcgc gtggtgttca
     7621 tgtcgtggaa gatgacctgc tcgcggcgct ggatagcggc aaagttaaag gcgcaatgtt
     7681 ggatgttttt aatcgtgaac ccttaccgcc tgaaagtccg ctctggcaac atccacgcgt
     7741 gacgataaca ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg agtacatttc
     7801 tcgcactatt gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg
     7861 cggctactaa taaagcatca ggattcctgc tatccttggc gggaattgaa tacaggagag
     7921 agttatgtat cccgtcgacc ttcatatgca taccgttgcc agcacacatg catatagcac
     7981 attgagtgat tacattgccc aggccaaaca aaagggcatt aaactttttg cgattaccga
     8041 tcatggcccg gacatggaag atgcgccgca tcactggcac ttcattaaca tgcgtatctg
     8101 gccgcgagtg gttgatgggg tagggatcct gcgcggcatc gaagctaaca ttaaaaatgt
     8161 tgatggtgaa attgattgca gcggtaaaat gtttgactcg ctggatctga ttattgccgg
     8221 ttttcatgag ccggtttttg caccacatga caaagcgacc aatacacaag cgatgatcgc
     8281 cactatcgcc agcggcaatg tgcatataat aagccatccc ggaaatccga aatatgagat
     8341 agatgttaaa gccgttgctg aagcagccgc gaaacatcag gtggcgctgg aaatcaataa
     8401 ttcctcattt ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagccgcagc
     8461 ggtacgtgat gctggaggtt gggtggcatt aggctcggat tctcacaccg cgtttaccat
     8521 gggggaattt gaagagtgtc ttaaaatcct cgacgcggta gattttccgc cagagcgcat
     8581 tttgaatgtt tctccgcgcc gcttactgaa cttccttgaa tctcgc
//
LOCUS       DBFIFS010000184         8544 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.184, whole genome shotgun sequence.
ACCESSION   DBFIFS010000184 DBFIFS010000000
VERSION     DBFIFS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.184"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            113..457
                     /locus_tag="N7A07_003735"
     CDS             113..457
                     /locus_tag="N7A07_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU2478321.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(461..2050)
                     /gene="yejF"
                     /locus_tag="N7A07_003736"
     CDS             complement(461..2050)
                     /gene="yejF"
                     /locus_tag="N7A07_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU2478322.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(2052..3077)
                     /locus_tag="N7A07_003737"
     CDS             complement(2052..3077)
                     /locus_tag="N7A07_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2478323.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(3077..4171)
                     /locus_tag="N7A07_003738"
     CDS             complement(3077..4171)
                     /locus_tag="N7A07_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU2478324.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(4172..5986)
                     /locus_tag="N7A07_003739"
     CDS             complement(4172..5986)
                     /locus_tag="N7A07_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU2478325.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(6068..7624)
                     /locus_tag="N7A07_003740"
     CDS             complement(6068..7624)
                     /locus_tag="N7A07_003740"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU2478326.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(7805..8371)
                     /gene="mepS"
                     /locus_tag="N7A07_003741"
     CDS             complement(7805..8371)
                     /gene="mepS"
                     /locus_tag="N7A07_003741"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU2478327.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
BASE COUNT         2240 a         2148 c         2142 g         2014 t
ORIGIN      
        1 gccacgcagg cgaggcatca atctttacga tctgtataaa gacggattgt tgatgatgtg
       61 ttaaaattga tgtaaacaaa ttgtgaagta aatgtgcttc cggggaaaat aagtgacttc
      121 attacaactc tcaatcgtcc atcgactgcc gcagaactat cgctggtctg ctggtttcgc
      181 aggttcgaag gttgaaccga ttccgcaaaa tggaccgtgc ggtgacaaca gcctggtggc
      241 gcttaaattg cttagcccgg atggtgataa tgcatggtcg gtgatgtata aactaagcca
      301 ggcgttaggc gacatcgaag ttccatgttc ggtgctggag tgtgaaggtg agccgtgcct
      361 gtttgtaaat cgccaggacg agtttgctgc aacatgccga ttgaaaaatt ttggtgtggc
      421 aattgccgaa cccttttcaa actacaatcc tttttaagcg tcagctcaac gccagtagct
      481 gacgcgtata ctcctgctgc ggtgtggcaa acacgcgcgc gcacggtcct tgttccacta
      541 cttccccttg tcgcagtacg ataacctgat gacataacgc gcggacaacg tgcaaatcgt
      601 ggctgataaa caaataggcc agttgatgct tttgttgcaa tgatttcaat agcgtcaata
      661 tttgcgcctg taccgttttg tcgagtgatg atgtcggttc atcaagtatg atcagcgagg
      721 gcttaagaat taatgccctg gcaatcgcaa tacgttgtcg ctgaccacca gagaactccg
      781 ccggataacg gtggcgtgtt tcaggatcta atcccacttc atgcatcacg tctatcactt
      841 gttgttcgcg ttgtgcggca gaaagcgtcg gctggtgaac ccgtaagcct tcctcaataa
      901 tctgcaaaac gttgagtcgt gggttgagcg aggagtttgg atcctgaaat accacctgaa
      961 tgcgatgacg aataggtaac agctggcggc gatttaaatt ttgcagtggc tgaccgtcaa
     1021 agatgatgct gccctgagaa ttaatcagtc gcagcagcgc cagtcccgtc gtgcttttcc
     1081 cggaaccgga ctcgcccact aaacccagtg tttcacccgc tcgtagcgta aaactgatgt
     1141 ttttcaccac cacattatga tccacaatgc gcttcaaaat ccctttgcga atggggaagg
     1201 caacctgaag ctgttcaaca tccagcaacg ttgaggcagg ttctggcaac ggcactggat
     1261 cgcctgacgg ttcactgttg agtagctttt gtgtgtaagg atgagtgggt gaggcaaata
     1321 gcgtagcggc gtaattttgc tcgacacagc gaccgttttg cattaccgcc acgcggtggg
     1381 ccagttttct gacaatgctg aggttatgag taataaacag catgcccata ttcaactcgc
     1441 cttgcagttc gcgcaacagc tgtaaaatct gcgcctggac agagacgtcc agtgcggtgg
     1501 tcggttcatc ggcaattaat aattccggtc gcgttaacag cgccatcgca atcatcaccc
     1561 gctggcgttc gccgccggag agctgatgcg gataatctgt cagccgtttt gccgcctggc
     1621 ggataccaac gcgatcaagg cagttaagaa tttcgccacg agccgcttcc cgacgcatcc
     1681 cgcggtggag tgaaagcact tcataaagct gtttttccag ggtatgcaat ggatttaacg
     1741 acaccatcgg ctcttgaaaa atcatggcga tcttattacc gcgtacaccg cgcaacgttt
     1801 ggtcgctggc atgaagcagc gattcgccat gaaaacgaat atcgccggag agatattcaa
     1861 ccggcgggga agggagcagg cgtaaaattg acagcgcggt aacgcttttg cctgaacctg
     1921 actcacccac cagcgccagc gtttcgccag cctcaatctg tagtgaaaca tcattgacta
     1981 ctgtacgtac ggtttgctga tggcgaaaac ccaccgacaa atcttcaatc gctaacagag
     2041 tttgcgtcat gctacaccgc cttattagga tcaaatgcgt cgcggacggc ttcaccaata
     2101 aagatcagca aagacaataa tatcgccacc gacaagaagg cggtgatccc aagccacgga
     2161 gcctgaaggt tatttttccc ttgtaacagc agttcaccga gtgacggtga accgagcggc
     2221 agaccgaagc cgaggaaatc gagtgaggtc agggtggtga tcgaactaca taaaataaac
     2281 ggtaaaaagg tgagggtagc gaccattgca ttcggcaaca tatgacgcag aataatactg
     2341 cgatcgctga cgccaagcgc ctgtgccgcg cggatgtagt cgaaattgcg ggtacgtaaa
     2401 aactccgccc gcaccacgcc gaccagactc atccagccaa ataagacagt aattgccagc
     2461 agccaccaga agttaggttg cacaacgctg gaaagcagaa taatcaaaaa cagcgttggc
     2521 atccccgacc atacttcaat aaagcgctgc ccccagagat cgaccttacc gccgtaatag
     2581 ccttgtagcg cccccgccag cacgcccatc acgctggaac agagagtcag catcaggcca
     2641 aacagaaccg agatccgcgt gccatagaga atacgtgcca gcacatcgcc gccgttggca
     2701 tccgttccca gccagttttg ccgggagggg ggagaaggga agggcttatc ggtagcaaag
     2761 ttgatactgg tagcaccaaa gcgaatcggt gcccacagta cccagccatt attttccagc
     2821 cgttgtttca gccacgggtc ctgataatca gcctgacttg ccagcgggcc gccaaaatcg
     2881 ctttcgctgt agtttttcaa caacgggaaa taccaactgc cgtcataacg caccagcaac
     2941 ggtttatcgt tggcgataag ttcagaacac aaactcaaac caaacaaaac gaggaaaatc
     3001 cataacgacc agtagccgcg acggttatgg cgaaaacgcg cccaacgggc ctgattgacg
     3061 gggttgagtc gcgacattaa cgtccctcaa aatctatacg cggatcgacc agcgtatagc
     3121 tgatatcact gacaatattc agcagcaggc caatcagggt gaaaatatac aaggtaccaa
     3181 acattacagg ataatcgcgc gagacggtcg cttcgtagcc cagtaagcca agaccattga
     3241 gtgaaaacat cacctcaatc agcagcgagc cggtaaaaaa catgctgata aacgtcgccg
     3301 gaaaaccggc aatcaccagc agcatggcgt tgcggaacac atgtttccag agaatatttt
     3361 tttcacttac ccctttcgca cgcgcggtca ccacgtattg cttgcgtact tcatcaagga
     3421 atgagttttt tgtcagcata gtcagcgccg caaagccacc aatcaccgtc gccagcaccg
     3481 gcagcgtgat atgccacaga taatcggtga ttttctgata ccacggcagc gaatcaaagt
     3541 tagcggaaac caggccgcgt agagggaaca ggtcgaaata gctgccgcct gcgaagaaga
     3601 caatcagcag gatggcaaac aaaaaggccg gaatggcgta gccgatgatg ataaatgcgc
     3661 tactccagac gtcaaagcgg ctcccattat aaacagcttt gcgaatgcct aacggaatcg
     3721 acaccagata gataatcagc gtgctccaca atccgagggt gatggaaacc ggcagactgt
     3781 ctttaatcag cgtcagcacc gaggcgctgc gaaacaggct atcgccaaaa tcaaagcgga
     3841 tgtagtccca gagcattttg aagtaacgtt cgtggatcgg cttatcgaaa ccgtagcgat
     3901 gagtgatctc ggcgatcact tctggatcta atccgcgtcc gccacggtaa ttactgtcgc
     3961 tgatattacc gacgcccgtt tgcgcatggc tggcgcgaac gccttcaccg cctgcgccgg
     4021 gtaatactcc ggcattacca aactcaatgg cggcgatggc ctggtcgacc ggaccgccag
     4081 gcgcaatttg cacgatgaaa aagttgatag tgataatcgc ccataacgtt gggatcacca
     4141 gcaacagacg gcgaatcaga taagcgccca tctactctcc ctgttgtctg gctgacggca
     4201 gtttggccgc tttattaaca tcataccacc aggtatcgat acccagactg taaacagggc
     4261 gcacagcggg ttgggagaat ttatcccacc aggcgagacg gtcttccgcc atgtaccaca
     4321 ttggcagcat gtaataattc cacgttaata cgcgatccag tgctcgccct aacggcagta
     4381 atttttcttt atttccctgc gcggcaataa tttggttgat cagcgagtcg ataaccgggc
     4441 tttgcacgcc gggggcatta taagtggaat tgatatattc cgatgaccag gaaatctgta
     4501 aatcggaact gggccacggc atcgcccgcc ataggcgcgg catcatgtca tagtcgcgac
     4561 tgcgcatgcg gttggtgatt tgcgagtgat ccaccttgcg aatgtccatg ttgataccca
     4621 gccgttgcag gctgtgctgg aacggcaata cccactgact attgctgctt gcaggaagca
     4681 ataattcaaa gctgagcggc tgacccgtag tggcattaac gcgttgctga cctttcagca
     4741 cccagcccgc ttcgttgaga agtttgtcgg cttttaacag gttgtcacga tcgtagccat
     4801 cgcctttcga taccggcggc tggtagattt gtgtgaagac ttctggcggt aaatcttttt
     4861 tcattggtgc cagaagcacc agctccgcgg cgtcggggta atttctggcc gcgtattcgg
     4921 tattctgaaa ataactgttc gtgcgactcc aggcattgta aaacaacgcc ttgttcatcc
     4981 attcaaagtc aaaggcgagg gtgattgctt cccggacccg gcgatcgctg aataccggac
     5041 gttggatatt aaacgccagc cagcgcgtat cctgggctga ttcgttcttt tgctcatctt
     5101 tgatgatgta ttttttatcg aaatttttac cgctatagcg tgtggcccag tttttggcgt
     5161 cgttttccat acgcaaatca aaggcacctg ctttaaacgc ttcaaaggcg acattatcat
     5221 cgaggtaata atcgtagcga atggtgtcga aattccagcg tccacggttt accggtaagt
     5281 ttgctgccca gtaatctttc acgcgggaat agacaatatt ttgccccatt ttccaggacg
     5341 taattcggta cggaccactg gcaagcggag gcgtggcgag cggatcgcta agtttgtgat
     5401 ccttccagta cttttctgga aataccggca gcgaaaacag actcaacata tcttctttgc
     5461 cgggtttagc taactcaatg cgcacggtta acggcgcaat ggctttgacg gtggtgcctt
     5521 tgtagaccag acgaaattgc ggcacgcctt cagtcataaa tttttgaaaa gtaaactcta
     5581 catcgcgggc agtaatgggc gaaccatcat gaaaacgggc gcgaggatta atagcgacct
     5641 ccacccagga ataatcgtca gcatagcgtg cgctttcagc aatcagcggg taataactgc
     5701 ctggttcgtc atcggaagtc gtaaatagcg tgtcgtacag ctgctcggtg cgtgcgcccg
     5761 ggttgccgcg cagtgcatag cggttgaaat tatcgaaggt accgagggct gacaacgtta
     5821 tctgcccacc ttttggcgcg gcggggttca cataatcaaa atggttgaaa ttaaacgcgt
     5881 accggggttc gcccagcacg gcaaaagcat agctttcctt gatagcctgc gcctgcgcac
     5941 caaaggtgaa cagagcgata aacagcagca gtatgcgcac aatcatctgc aacggatttc
     6001 cttttactca tcattagccg caatccggca tgctttcact atgaataata agggacgtaa
     6061 gcacacctta ccactgcggc gtatacggtt tcttcagcca gcggacaaaa tcgtccagcg
     6121 gtaacgggcg actaatccag tagccttgca tgaaattaac gccgcgttcg cttagccatc
     6181 gcgcctgttc cggcgtttcg accccctcag caaccgtcag catattgagg cgtttcgcca
     6241 gcgtcagcac cgcgtcaagt acgggtgaag tgatcgtttc cgtaccgatg gcgttgataa
     6301 atccacggtc aattttcaga taatcgagcg taaaacgctc aagatagata agcgcgctgt
     6361 gcccggtgcc gaagtcatca atagcaattt ctacgccgac cgagtgcagc caggcgaaga
     6421 gttgtgtggc ttcttgctct ttcagcatat cgcgctcggt aatttccagc acaatctgga
     6481 aatggtgtgc gggtagggaa gtgagcagtt tctggatatt tgctttaaag ctttcgctgt
     6541 gcagatggtc cggcgcaatg ttaatgccaa atttgacgcc taccggcagc actttttcta
     6601 attctgcggc atcgcgggca attaactcaa acaggtgctg agtcagcggc acaatcatct
     6661 tttgcgattc ggcaaagtta atgaaggcat ccgggggaat ttctcccgcg acaggatgcc
     6721 gccagcgtag cagtacttcc aggcccgtta ctcgcaaagc ttgtgtatcc accaccggtt
     6781 gatacgccac gtaaaattgt tcgcgcttga tggcggtcat gatttctctg ccggggcgca
     6841 tacgtacgct catcaggtaa tagcagagca ggccaacgac agttccactc atgccgccca
     6901 gtaaaaatgc gtaccacaca tcgttccatg tccagtcatc tgcataaagc cgaacggtca
     6961 gaggaatgcc cgcaatttta gtttcacgga ctggcatgtc ggttaattcg ttaacgttca
     7021 tcaaacgtga agaaaaggta gatagcgcag tattgccaat aatgagggcg acgccatcgt
     7081 aatcttcctg ccgtgagcta taaaagagag aaggcgtcag gttgagattc agagcggcaa
     7141 agacgccgct atttttcagc aaagggttgc gataccagat gacgattgcg ggtttgttcg
     7201 gcaccatcgg cgtgccgggt aagatcgcca tatcgacatt tttattaatg tcgagcgcag
     7261 gaatcaattc attgagtgga atgtccatct caccggtcgc agatgaacag aatgtttttt
     7321 tatctttcac cagcacaaac gttcggacat tcatgctaaa cgccgcgcgg gcggtcagtt
     7381 cggggttagc ttgcttgcat gtatctaagg tcagcggctg gagccggtca gtagtggatt
     7441 tcaggtcggc aaaataggta tcgagatact tttgtacgtc ggtaatcagt gtgtcgtatt
     7501 tgacttcgtg cttatgccag gccactaaaa actgaaggca gctcaccagt atcgcaatca
     7561 ttacgcctgc aacaatgcag gtaagcagga gcttacgtcc agaattggga gcgcgtatga
     7621 acatgattcc gttaatttcc tgaaattacg acaacattta aaagataaca atgaccggac
     7681 tatagcctgg atcaagacgc ggaaacatca ttttctctga tgaatgaaaa caaaaaagca
     7741 ctgcctaaac agtgctttca gttaactcgt caggatagcc aagggattgc atccaaacgg
     7801 tttattagct gcggctgaga acccggcgtg cttcgttgta acgcttcttc cagtacggtt
     7861 cattcatgct ggaaataata acaccactgc tggtggaagc atggacaaac tgattgttgc
     7921 cgatataaat accgacatgg cgtcccgttg aaccggcacg gaacagaact aaatcacccg
     7981 tacgcaaatt actgcgggaa acagatttac ccatttcctg ctgttcgtaa gtcgaacgcg
     8041 gaagttctaa gccaaattgc tcacggaatg tacgctgtac gaaaccagaa caatcgatac
     8101 cttttttagt gctgccgccc agacgataac gtacgccttt ccagtcagca tactgatcca
     8161 taattcgcga ttttacgtcg acattacgaa ccaggttttc aaattcatcc tgagaagctt
     8221 gcagtgatga tgtttcacta cccactgcac gtgtctcagg atgcatattc tttgcggtgt
     8281 tatttgcact acatgcagaa agcagaaccg ctactgcaat cgcggggatc ccgcgcaaga
     8341 tatatctcaa aatcggttga gatttgacca tgttgtttgt tttcccttga agtccttaac
     8401 gacaaatatc gttataaaaa aaatgccaag cgacacgaga ctaattacca gggcatgttg
     8461 aaacaattcc ttaaggccaa atctgtggac taacgcacaa aattatatgc tgtgaggata
     8521 atctaccgcc gaggcaaaac gagt
//
LOCUS       DBFIFS010000185         8525 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.185, whole genome shotgun sequence.
ACCESSION   DBFIFS010000185 DBFIFS010000000
VERSION     DBFIFS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.185"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            95..214
                     /locus_tag="N7A07_003742"
                     /pseudo
     CDS             95..214
                     /locus_tag="N7A07_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(225..473)
                     /locus_tag="N7A07_003743"
     CDS             complement(225..473)
                     /locus_tag="N7A07_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2478328.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(535..1632)
                     /locus_tag="N7A07_003744"
     CDS             complement(535..1632)
                     /locus_tag="N7A07_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2478329.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            1721..2758
                     /gene="dusA"
                     /locus_tag="N7A07_003745"
     CDS             1721..2758
                     /gene="dusA"
                     /locus_tag="N7A07_003745"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU2478330.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     LHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2892..3134
                     /gene="pspG"
                     /locus_tag="N7A07_003746"
     CDS             2892..3134
                     /gene="pspG"
                     /locus_tag="N7A07_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU2478331.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(3300..4283)
                     /gene="qorA"
                     /locus_tag="N7A07_003747"
     CDS             complement(3300..4283)
                     /gene="qorA"
                     /locus_tag="N7A07_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU2478332.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4366..5781
                     /gene="dnaB"
                     /locus_tag="N7A07_003748"
     CDS             4366..5781
                     /gene="dnaB"
                     /locus_tag="N7A07_003748"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU2478333.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5834..6913
                     /gene="alr"
                     /locus_tag="N7A07_003749"
     CDS             5834..6913
                     /gene="alr"
                     /locus_tag="N7A07_003749"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU2478334.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            7166..8359
                     /gene="tyrB"
                     /locus_tag="N7A07_003750"
     CDS             7166..8359
                     /gene="tyrB"
                     /locus_tag="N7A07_003750"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU2478335.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2031 a         2048 c         2178 g         2268 t
ORIGIN      
        1 gattgtagga agagataatt gtgcttttga ccttgacaga ggtaacttcg ttttaaaaaa
       61 ttaagatagc aatggcgggt taacttttcg taaaatgaaa ttaattctcg gtacgacatg
      121 taaaacgcgg cacctcgtat gcaagaacgt actgcggttg gctggtgaac tttcgatagt
      181 gctagtattg aatgatttcc agccgttacc gatttacgtg tttattagtg aacaaaccac
      241 tcgtcagaag attcccaggt atctttcaga gtctcctgaa caaatgtttt tgcagaatcc
      301 ttatctgcag tgcgtgtaac agaaagccca tcattgctgg tggcttttac gatcacttct
      361 acatcgtcat aacgcttact gatgcgccga gttaattctt cctttaacgc atccacagca
      421 ccgtttggca ttttagtcat tttctctttg gctatgcaga tttcaatacg cataaaaatc
      481 cctctatact gtgtttgtat acagtgttat ttttatctgt ataaataaac agtgtcaaga
      541 ggtcttgttt ctgctctttt ggagttcttc aaaacgatta tgtaaagatt tcggatacag
      601 ttcggtatat acctgccata gcacgtttaa tgaacgatgc cctgtaactt gagcgacttc
      661 ctcaatacta aaaccagcct caaataagcg acttgcccct tctctacgca aatcatgata
      721 tcgcagatct ttaataccta atttgcttct taccctctga aatcctgcgg taacagaagt
      781 gctgttatat ggaaaaatga attctgattt ttttggttgt cgctggacga tatcccaggc
      841 ttccccaagc aaggcaactt tcatatggtt accttccttt ttacgtggat ctttcctatc
      901 tcttactagt atagattttt gttcttggtc gagatcttcc catcgtaacc ggcatacttc
      961 tccgattcgc atacaggacc acacagaaaa tttgaggata tcaacgaacg gaatttttga
     1021 gcatttatga gtagatcgtt gttgaaggcc ttcaatgagc atgtcaagtt catcagatgc
     1081 tggtctacga ttacgacggt ttgatttacc aatcaaaccg agtttaagta gatatggacg
     1141 agcgcttttc gccgggtttg atgtgtaatt gattccgtat acaggttttg ccgcatccag
     1201 aacactgcca agataactaa catcgtggct gactgttgct gggcctgcac cagcgttgtt
     1261 tcttagcctg caatgttcaa ttacgtcatt ttctgtcagt tcagatagtt tgatcgcgga
     1321 tatgtcacta tccataagca gttccagcac atatctttta gtacggcctg ctttacctcc
     1381 ggcatttggg tcatttaaat atttgtgtag taagtcacgg actgtaagtc cgtcaactgc
     1441 atttgatgat ggaatgccat atagatctaa ttccatcact ttctgtgtgc cccatgtttt
     1501 ggcatgagca tgtttaggga atgttttgct ttccctgtaa gtgataacac ctttttcttt
     1561 gataatcaca ttacagcgat agcgtggtgt gccatcggat tttagtcgtt tctctatgtt
     1621 atagtacgcc attacacgac ctcgttattt cgggttccca taaaacgtgg gaacctgtgc
     1681 gggaacctaa cgcgagaaaa atagcctgaa atgttcaaaa atgcacgata atcatgaagc
     1741 acaaaaaatt aatcaaacca gcgtgatgcc tgaaaaaact ggtgtttact ggaattctcg
     1801 gtttagcgtt gcaccaatgc tcgactggac ggacagacat tgccgctatt tcttgcgtct
     1861 gctttcccgc aatacgttgc tgtataccga aatggtgact acaggggcga ttattcacgg
     1921 taaaggtgat tacctggcgt acagtgaaga agaacatccg gtagcgttgc aactcggcgg
     1981 tagcgatccg gcggcgctgg cgcagtgtgc gaagctggca gaagcgcgtg gatatgatga
     2041 gatcaacctg aatgtcggct gcccgtctga ccgggtgcag aacggcatgt ttggtgcgtg
     2101 tctgatgggt aatgcgcagc tggttgccga ctgcgtgaaa gcgatgcgcg atgtggtgtc
     2161 gattccggtg acggtgaaaa cgcgtattgg catcgatgac caggacagct atgaatttct
     2221 ctgcgatttc atcaacaccg tttccggcaa aggcgagtgt gagatgttca tcatccacgc
     2281 acgtaaagcc tggctttcgg ggttaagccc gaaagaaaac cgtgaaatcc cgccgctcga
     2341 ttatccgcgt gtgtatcaac tgaagcgtga ctttctgcat ctgacgatgt cgattaacgg
     2401 tggtatcaag tcgctggaag aggctaaagc gcatttgcaa catatggatg gcgtgatggt
     2461 cgggcgcgaa gcgtatcaga atccgggtat tctggcggcg gtagaccgag agatctttgg
     2521 ttcctcggat accgatgccg atccggtggc ggtagtgcgc gccatgtatc cgtacattga
     2581 gcgtgaactc agccagggga cgtatcttgg ccatattacc cggcatatgt tgggcttgtt
     2641 ccagggtatt cctggcgcgc ggcagtggcg gcgttattta agtgaaaatg cccataaagc
     2701 gggtgcagac attaacgtgc tggaacacgc gctcaaactg gtggcggata agcgttaact
     2761 tttcaccaaa aagtagtcaa attcaccacg ccctgcgcac cgtcgcgggg cgttttgctg
     2821 ttaaatcaat agattatttt tggcatgatt cttgtaatgc ctgcaagaga tttcatattt
     2881 gggagagcat catgctggaa ctactttttg tgattggctt ttttgtcatg ttgatggtca
     2941 ccggcgtttc gttgctgggc attatcgccg cgctggttgt ggcgacggcc attatgttcc
     3001 tcggcggtat gctggcattg atgattaagt tgctgccgtg gttactcctg gcgattgcgg
     3061 tggtgtgggt tattaaggcg attaaagcac caaaagtgcc gaaatatcag cgttatgacc
     3121 gctggcgtta ctaagggatt gtgcggatga tcacaaccta aggttttatc cttagaacaa
     3181 aataggaatt gataatcaaa tctgtcacta ttgcgcctct aacagattca tcgtgctgta
     3241 ccctacatac agccgaacta taaaaagaaa gggcttccca ggtggaagcc ctatttcttt
     3301 tatggaatca acaggctgga accttgcgtc gcgcggcttt ccagaatctc atgcgcacgc
     3361 tgcgcatcct tcagcggata tttctgctgc tcggcgacat cgaccttaat cacaccgctg
     3421 gcaatcaaag agaacagttc attactggcc tcggttaatt cctcccgcgt ggtgatatag
     3481 ccttgcaggg aagggcgtgt cacatacaac gagcctttct gattgagaat gcctaagttc
     3541 acaccggtaa ccgcacctga tgagttgcca aaactcacca ttaagccgcg gcgttgcagg
     3601 caatccagcg accgttccca ggtgtctctg cccacggaat cgtacactac gcgcactttt
     3661 ttaccaccgg tgatctcttt taaccgctcg accagattct cttcacgata gttaataacc
     3721 tgccacgcgc ccgcttttag cgcgctctgc gctttttgcg cggttcctac ggtgccgata
     3781 agtttcgcgc ccagggcttt tgcccactgg caggcaatca agccaacgcc gccagctgct
     3841 gcgtggaaca ggaactgttc atcgggttta atttcatagg ttttgcgcag cagataataa
     3901 accgttaagc ctttcaggaa agatgctgca gcttgctcaa aagaaattgc cgcaggcaga
     3961 atcgccgctt tatccgcatt aatgttatgc acagagctgt aagcgcctaa cgccgactgc
     4021 gcatagacta cacgatcgcc tgccttaata tgctttacac cactgccgac tttactcacg
     4081 atgcctgccg cttcggtacc taatccgctg ggtagtgatg gcggcgggta aaggccgctg
     4141 cggatatacg tgtcgataaa attgatgccg atggctttat tttcgacctg gatttcattc
     4201 tccgccggat cggcaggagt gaactctacg gcttgaagta cttccgggcc accgtgcttg
     4261 tgaaattcaa ttcgtgttgc catgtgtcct ccagaacgta tcgtcagggt ctgcttcata
     4321 tggtaaagtt tcgacccatt ctttatctcg gtaactccat tcactatggc aggaaataaa
     4381 cccttcaaca aacagcaggc tgaaccccgc gaacgcgatc cacaagttgc cgggctgaaa
     4441 gtgcctccgc actcgatcga agcggagcag tcggtgttgg gcggtttaat gctggataac
     4501 gaacgctggg atgatgtagc cgagcgtgtg gtagcagacg atttttacac ccgcccacac
     4561 cgtcatatct ttactgaaat ggcgcgtttg caggaaagcg gtagtcctat cgatctgatt
     4621 acccttgcgg aatcgctgga acgccagggg caactcgata gcgtcggtgg ttttgcttat
     4681 ctggcagagt tgtcaaaaaa tacgccaagt gctgcgaaca tcagttccta tgcggacatc
     4741 gtgcgtgaac gtgccgttgt ccgtgagatg atctcggttg cgaatgagat tgccgaagct
     4801 ggttttgatc cgcaggggcg taccagcgaa gatctgctgg atctggctga atcccgcgtc
     4861 tttaaaattg ccgaaagtcg tgcgaacaaa gacgaagggc cgaagaacat cgccgatgtg
     4921 ctcgacgcaa ccgtggcgcg tattgagcag ttatttcagc agccacacga tggcgttacc
     4981 ggggtaaaca ccggttatga tgatctcaac aaaaaaaccg ctggcttgca gccgtcggat
     5041 ttgatcatcg tcgccgcgcg tccgtcgatg ggtaaaacaa catttgcgat gaacctcgtc
     5101 gaaaacgcgg cgatgttgca ggataaaccg gtacttattt tctcgctgga gatgccatca
     5161 gaacagatca tgatgcgttc tctggcgtcg ctgtcgcgcg ttgaccagac taaaatccgt
     5221 accgggcagc tcgatgatga agactgggcg cgcatttccg gcaccatggg tattttgctc
     5281 gaaaaacgca atatctatat cgatgactcc tccggcctga cgccaacgga agtgcgttcc
     5341 cgcgcacgcc gtattgcccg tgaacacggc ggcatcgggc ttatcatgat cgactacctg
     5401 caactgatgc gcgtaccggc actttccgat aaccgtacgc tggaaattgc agaaatctcc
     5461 cgctcgctga aagcactggc gaaagaactg aacgtgccgg tggtggcgct gtcccagttg
     5521 aaccgttctc tggaacaacg tgccgacaaa cgtccggtca actccgacct gcgtgaatct
     5581 ggctctatcg agcaggatgc ggacttgatc atgtttatct atcgtgatga ggtgtatcac
     5641 gaaaacagtg atttaaaagg catcgcggaa attattatcg gtaaacaacg taacggccca
     5701 atcgggacgg tacgcctgac ctttaacggt caatggtcgc gcttcgacaa ctatgcgggg
     5761 ccgcagtacg acgacgaata ataattattt tatgaattag gtaattaaag caaacactta
     5821 tcaaggaaca caaatgcaag cggcaactgt tgtgattaac cgccgcgctc tgcgacacaa
     5881 cctgcaacgt cttcgtgaac tggcacctgc cagtaaaatg gttgcggtgg tgaaagcgaa
     5941 cgcttatggt cacggtcttc ttgagaccgc gcgaacgctc cccgatgctg acgcctttgg
     6001 cgtagcccgt ctcgaagaag ctctgcgact gcgtgcgggg ggaatcacca aacctgtact
     6061 gttactcgaa ggcttttttg atgccagaga tctgccgacg atttctgcgc aacattttca
     6121 taccgccgtg cataacgaag aacagctggc tgcgctggaa gaggctagcc tggacgagcc
     6181 ggttaccgtc tggatgaaac tcgataccgg tatgcaccgt ctgggcgtaa ggccggaaca
     6241 ggctgaggcg ttttatcatc gcctgaccca gtgcaaaaac gttcggcagc cggtgaatat
     6301 cgtcagccat tttgcgcgcg cggatgaacc aaaatgtggc gcaaccgaga aacaactcgc
     6361 tatctttaat accttttgcg aaggcaaacc aggtcaacgt tccattgccg cgtcgggtgg
     6421 cattctgctg tggccacagt cgcattttga ctgggtgcgc ccgggcatca ttctttatgg
     6481 cgtctcgccg ctggaagatc gctccaccgg tgccgatttt ggctgtcagc cagtgatgtc
     6541 actaacctcc agcctgattg ccgtgcgtga gcataaagtc ggagagcctg tcggttatgg
     6601 tggaacctgg ataagcgaac gtgatactcg tcttggtgta gttgcgatgg gctatggcga
     6661 tggttatccg cgcgccgcgc cgtccggtac gccagtgctg gtgaacggtc gcgaagtgcc
     6721 gattgtcggg cgcgtggcga tggatatgat ctgcgtagac ttaggtccac aggcacagga
     6781 taaagccggg gacccggtca ttttgtgggg tgaaggtttg cccgtagaac gtatcgctga
     6841 aatgacgaaa gtaagcgctt acgaacttat tacgcgcctg acttcaaggg tcgcgatgaa
     6901 atacgtggat taatcgttct gtaatatttg attgtctgtg ccggatgcgg cgtgaatgcc
     6961 ttatccggcc aataaaatcc taccaattca ataagttgat attctttcat gctcttataa
     7021 aggtcgtgcc tctggcggat gtacgtttgt catgagtctc actctgttgc taattgccgt
     7081 tcgcccctga acatccactc gatcttcgcc ttcttccggt ttattgtgtt ttaaccacct
     7141 gcccgtaaac ctagagaacc atcgcgtgtt tcaaaaagtt gacgcctacg ctggcgaccc
     7201 gattcttacg cttatggagc gttttaaaga agaccctcgc agcgacaaag tgaatttaag
     7261 tatcggtctg tactacaacg aagacggaat tattccacaa ttgaaagccg tggcggaggc
     7321 ggaagcgcgc ctgaatgcgg tgcctcatgg cgcttcgctt tatttaccga tggaagggct
     7381 taacagctat cgccatgcca ttgcgccgct gctgtttggt gccgaccatc cggtactgca
     7441 acaacagcgc gtagcaacca ttcaaaccct tggcggctca ggggcattga aagtgggcgc
     7501 ggatttcctg aaacgctact tcccggaatc aggcgtctgg gtcagcgatc ctacctggga
     7561 aaaccacgta gcaatattcg ccggggctgg attcgaagta agcacttacc cctggtatga
     7621 cgaagcgact aacggcgtgc gctttaatga cctgttggcg atgctgaaaa cattacctgc
     7681 ccgcagtatt gtgttgctgc atccatgttg ccacaaccca acgggtgccg atctcactaa
     7741 tgaccagtgg gatgcggtga ttgaaattct caaagcccgc gagcttatcc cattccttga
     7801 tattgcctat caaggatttg gtgccggtat ggaagaggat gcctacgcca ttcgcgccat
     7861 tgccagcgct ggattacccg ctctggtgag caattcgttc tcgaaaattt tctcccttta
     7921 cggcgagcgc gtcggcggac tttctgttct gtgtgaagat gccgaagctg caggccgcgt
     7981 actggggcaa ttgaaagcaa cagttcgccg caactactcc agcccgccga attttggtgc
     8041 gcaggtggtg gctgcagtgc tgaatgacga ggcattgaaa gccagctggc tggcggaagt
     8101 agaagagatg cgtactcgca ttctggcaat gcgtcaggaa ctggtgaagg tattaagcac
     8161 agagatgcca gaacgcaatt tcgattatct gcttaatcag cgcggcatgt tcagttatac
     8221 cggtttaagt gccgctcagg ttgaccgact acgtgaagaa tttggtgtct atctcatcgc
     8281 cagcggtcgc atgtgtgtcg ccgggttaaa tacggcaaat gtgcaacgtg tggcaaaggc
     8341 gtttgctgcg gtgatgtaat gcaggaaagc aggctggaat tacccagcct gcagtgaaat
     8401 taaactgtcg tcgctttcac tctttcttta tagatgattt ttttgatacc atcgttctac
     8461 gtgagagata ataaacgttg ttagttcttt tattgttaag tttatcccaa ttatctggaa
     8521 ttcct
//
LOCUS       DBFIFS010000186         8456 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.186, whole genome shotgun sequence.
ACCESSION   DBFIFS010000186 DBFIFS010000000
VERSION     DBFIFS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.186"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            137..2515
                     /gene="ppsA"
                     /locus_tag="N7A07_003751"
     CDS             137..2515
                     /gene="ppsA"
                     /locus_tag="N7A07_003751"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU2478336.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2572..4217)
                     /gene="fadK"
                     /locus_tag="N7A07_003752"
                     /pseudo
     CDS             complement(2572..4217)
                     /gene="fadK"
                     /locus_tag="N7A07_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(4274..4567)
                     /locus_tag="N7A07_003753"
     CDS             complement(4274..4567)
                     /locus_tag="N7A07_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU2478337.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4564..5853)
                     /locus_tag="N7A07_003754"
     CDS             complement(4564..5853)
                     /locus_tag="N7A07_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2478338.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5909..6847)
                     /locus_tag="N7A07_003755"
     CDS             complement(5909..6847)
                     /locus_tag="N7A07_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU2478339.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6867..7631)
                     /locus_tag="N7A07_003756"
     CDS             complement(6867..7631)
                     /locus_tag="N7A07_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU2478340.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7947..>8456
                     /locus_tag="N7A07_003757"
     CDS             7947..>8456
                     /locus_tag="N7A07_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU2478341.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYR"
BASE COUNT         2015 a         2165 c         2196 g         2080 t
ORIGIN      
        1 tgggatcagt cttaacaagt aaaaaaatat atttgcttga acgattcacc gtttttttca
       61 tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt tctcaaaccg ttcatttatc
      121 acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg ctggtgcttt ggtataacca
      181 actcggcatg aaggatgtag acagggttgg gggcaaaaat gcctccctgg gtgaaatgat
      241 tactaacctt tccggaatgg gtgtttccgt tccgaatggt ttcgccacaa ccgccgacgc
      301 gtttaaccag tttctggacc aaagcggcgt aaaccagcgc atttatgaac tgctggataa
      361 aacggatatt gacgatgtta ctcagcttgc gaaagcgggc gcgcaaatcc gccagtggat
      421 tatcgacact cccttccagc ctgagctgga aaacgccatc cgcgaagcct atgcacagct
      481 ttccgccgat gacgaaaacg cctcttttgc ggtgcgctcc tccgccaccg cagaagatat
      541 gccggacgct tcttttgccg gtcagcagga aaccttcctc aacgttcagg gttttgacgc
      601 cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt aacgatcgcg ccatctctta
      661 tcgtgtgcac cagggttacg atcaccgtgg tgtggcgctc tccgccggtg ttcaacggat
      721 ggtgcgctct gacctcgcat cttctggcgt gatgttctcc attgataccg aatccggctt
      781 tgaccaggtg gtgtttatca cttccgcatg gggccttggt gagatggtcg tgcagggtgc
      841 ggttaacccg gatgagtttt acgtgcataa accgacactg gcggcgaatc gcccggctat
      901 cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt tacgcgccga cccaggagca
      961 tggcaagcag gttaaaatcg aagacgtacc gcaggaacag cgtgacatct tctcgctgac
     1021 caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa attgagaaac actacggtcg
     1081 cccgatggat attgagtggg cgaaagatgg ccacactggt aaactgttca ttgtgcaggc
     1141 gcgtccggaa accgtgcgct cacgcggtca ggtcatggag cgttatacgc tgcattcaca
     1201 gggtaagatt atcgccgaag gccgtgctat cggtcatcgc atcggtgcgg gtccggtgaa
     1261 agtcatccat gatatcagcg aaatgaaccg catcgaacct ggtgacgtgc tggtcactga
     1321 catgaccgac ccggactggg aaccgatcat gaagaaagca tctgccatcg tcaccaaccg
     1381 tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa ctgggcattc cggcggtagt
     1441 gggctgtggt gatgcaacag aacggatgaa agacggtgag aacgtcactg tttcttgtgc
     1501 cgaaggtgat accggttacg tctatgcgga gttgctggaa tttagcgtga aaagctccag
     1561 cgtagaaacg atgccggatc tgccgttgaa agtgatgatg aacgtcggta acccggaccg
     1621 tgctttcgac ttcgcctgcc tgccgaacga aggcgtgggc cttgcacgtc tggaatttat
     1681 catcaaccgt atgattggcg tccacccacg cgcactgctt gagtttgacg atcaggaacc
     1741 gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt gattctccgc gtgaatttta
     1801 cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc gcgttttatc cgaagcgcgt
     1861 cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc aacctggtcg gtggtgagcg
     1921 ttatgagcca gatgaagaga acccgatgct cggcttccgt ggcgcgggac gctatgtttc
     1981 cgacagcttc cgcgactgtt tcgcgctgga gtgtgaagca gtgaaacgtg tgcgcaacga
     2041 catggggctg accaacgttg agatcatgat cccgttcgtg cgtaccgtag atcaggcgaa
     2101 agcggtggtt gaggaactgg cgcgtcaggg gctgaagcgt ggtgagaacg ggctgaaaat
     2161 catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc gagcagttcc tcgaatattt
     2221 cgacggcttc tcaattggct caaacgacat gacgcagctg gcgctcggtc tggatcgtga
     2281 ctccggcgtg gtgtctgaac tgttcgatga gcgcaacgat gcggtgaaag cactgctgtc
     2341 gatggcgatt cgtgccgcga agaaacaggg caaatatgtc gggatttgcg gtcagggtcc
     2401 gtccgaccac gaagactttg ccgcatggtt gatggaagag gggatcgata gcctgtctct
     2461 gaacccggac accgtggtgc aaacctggtt aagcctggct gaactgaaga aataaaataa
     2521 atccccggcg gcgttttgtc gccgggttta tgtgatcccc gaagatgaaa cttattcaat
     2581 ctcttcacag acatcctgcg ttaatcgccg cataatatct ttccgtaaca aaaacttttg
     2641 tattttacct gaggcagtgc gcggtagttt ttcgattacc acgatatgtt caggatattt
     2701 atattttgcg acccgtttac ggctaaaaaa gccaccacct cttccagcga taatgaatga
     2761 tgcggcgctt tcagcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt
     2821 gcaaccacac aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg
     2881 ctgctaatat tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata
     2941 tagccagcct catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagg
     3001 gcacgggcgg ttaattcagg ttcatcaaaa tatcccataa acacattggg gccacgcgag
     3061 gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc
     3121 tctacacctg cggcagcgta accatcggtg tgcataaagc gcgacaaagg atcatcgaga
     3181 ttcaccaccg catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgccg
     3241 cgctgctggc attcacgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag
     3301 cgcagcgctg aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac
     3361 ggcgttgcgc cgagcataca ggagcaacgc tgctgctcaa gcagcgcgag acacgcatca
     3421 ggagtgaaaa tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca
     3481 tgcagaaagc ccgttgcgtg accaagtggc gcaggcatca taaagacatc ctgccaggtt
     3541 agattcagcc gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgtaagcatc
     3601 acgccctttg gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg
     3661 tgggtcgtta tcgccgtggt cagtggggta ttgtcggcga taatctgact taatgagagg
     3721 gaagaggtgg cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga
     3781 ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgtaaag
     3841 aacatttttg cctgacactt attaagcacc cataccagtt ctgcttcccg ccaggaaggc
     3901 aacagcggta cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca
     3961 caccagcctg gcagttgaaa tgctatgcga tcgcctgatt caataccctt cgctaacatc
     4021 cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgctccatga
     4081 ttatcgacca cggcaatttt gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc
     4141 agcgaagcat cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac
     4201 gttaatgtca ctttcatttt ggactctcga aacagaacat caggcccgag atgcgggcct
     4261 gtgggatgca tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc
     4321 actgttcgag aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg
     4381 cggaatcaaa atgaatgttt cctgcgtcat cctgcttata aagtccggca gggcaggctt
     4441 tcattaattt acggaattca ttgatatcgg gatttgccgc caaaatgata tgcgggtggc
     4501 cctcatcaac atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct
     4561 ggctcatagc gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt
     4621 cttcgcgtgt cccatgatca ttttgcgtac cggctggtta ggcttaccgt caatggtgaa
     4681 catctcgttc atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc
     4741 catcagcgcc gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag
     4801 ctcacgtttg tattgcgcca gactgctggc ggagaaatcc gcgcgttctt tggcggcgat
     4861 cactgttgtg gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa
     4921 acccaaattc aggcagaagc ctgcggcgtc accaacgatc atcacgccat cgttaaccag
     4981 ttgcggtacc atcgccagac cgccttctgg caccatatgc gcggaatatt caagcagttt
     5041 gccgccgcta atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg
     5101 cacgcttttt tgcgcatggg cgatatcagc caatccacaa accagcccca aggatatgga
     5161 atccttgttg gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa
     5221 cagccaggcg gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt
     5281 gaggccaata acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat
     5341 tcccagcgag cggccaagca tcgagttaac gccatcggct aaaatcacca cattggcttc
     5401 gagaatatca tccccggcct ggactccagt gaccttgttt ccttcacgaa ccaacgcatc
     5461 gacgcgaact cccgggataa actgtgcgcc cgcctgctcg gcttgttcca tcaaccacgg
     5521 gtccagacga ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg
     5581 gtgaaaatcg agggtaacgg cgctctcttc ggttaagaag gagattttct cgcgtgtgac
     5641 cttgcgttct accggcgctg atgctgcaaa gcctggaatg attgcttcaa gtgtgtgggc
     5701 ataaagacgc ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag
     5761 gatatccagc ccggctcgcg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc
     5821 gaccacaatg gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt
     5881 acagcgggag atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga
     5941 cgactttgta gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt
     6001 tatctttatt gatggcgaca atcacttttg cgccgttgcc gccaaccata tgctggatct
     6061 gcccggagat ccctagcgtc aggtagagat cggatttcag caacacgccg gagacaccga
     6121 tataacgttc acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt
     6181 cagcattcag caccgccgcc agttcgtgga ccattttcag gtcatcctgc gctgccagac
     6241 cacgaccgac gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg
     6301 cacggcgttc gcgacagaga atttcatgac gcggagcaac gtaaggtacc gtttctgtcg
     6361 ggcactgatg agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga
     6421 tcgccagcga gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt
     6481 cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat
     6541 tcagttgcac acttaaccgt gctgccagcg ctttaccacg tttcgtcgcg gccagtagca
     6601 acatagcggg gtgtttatct ttcagcagag cagcaatgct ttcggcgtaa ttttcagtgc
     6661 gttgcagcgc gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg
     6721 cctgcgcctg gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc
     6781 cgccaaacag ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt
     6841 gactcatcgt ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc
     6901 tcggcaatgg cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc
     6961 ggtacgcgaa tgccggtcag ttcagcaagt ggtgcgctct ggctccagcc aatatcactt
     7021 gcctgccact gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg
     7081 cgtggcacgt taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact
     7141 tcaacgtcat cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca
     7201 ctcactgcat taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg
     7261 gcataaaggt cgccggaacc tttaccaaag atcagtaaat cgaagccgat cttttcaact
     7321 gctgccgcca gcgcttttgc ggtatcgaga ggcagtgcat gctcaagttg tgcatcctgc
     7381 accatataca ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc
     7441 aacaatgagc cgccaacggt cagcgcggct atctcatcgt catctgttgc aagctggctt
     7501 gcagcttcaa tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc
     7561 agggtgtatt ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt
     7621 attattttca tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa
     7681 atggcctgaa aatagtgtgc gatagcggtg cggggttttc gacggagcaa gctgttttcc
     7741 ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca aaagtgcaat ttgcggcaat
     7801 atagttattc catgaccgta caatcttgca ttcagtgtaa ataatcttgt ttagctctcg
     7861 ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta tccggcatat attgtttttg
     7921 cttgctcatg gaatagggag gttgtcatgt atcaacgctg ttttgataat gccagtgaaa
     7981 cgctgtttgt cgctggtaaa acgccacggc tttcacgttt tgcatttagc gatgatccaa
     8041 aatgggagtc tggacatcac gttcatgaca atgaaaccga gctgatttac gtcaagaaag
     8101 gggtggcaag gtttaccatc gattcttcgt tatatgtcgc gcatgcagat gatatagtgg
     8161 tgatagaacg cggcaggctg catgcggtgg cctctgacgt taacgatccg gcaacgacgt
     8221 gtacttgtgc gctgtacggc tttcagtttc agggggctga ggaaaatcat ctactgcaac
     8281 cgcattcttg tccggtaatt gccgcggggc agggaaaaga agtcattaaa accttattta
     8341 atgagctaag tgtgattttg ccgcaaagta aaaatagcca aacatcttcg ttatgggatg
     8401 catttgccta tacattagca attctttact acgaaaactt taaaaatgct tatcgt
//
LOCUS       DBFIFS010000187         8437 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.187, whole genome shotgun sequence.
ACCESSION   DBFIFS010000187 DBFIFS010000000
VERSION     DBFIFS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.187"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            137..1240
                     /gene="ompC"
                     /locus_tag="N7A07_003758"
     CDS             137..1240
                     /gene="ompC"
                     /locus_tag="N7A07_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU2478342.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1352..2407
                     /gene="apbE"
                     /locus_tag="N7A07_003759"
     CDS             1352..2407
                     /gene="apbE"
                     /locus_tag="N7A07_003759"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU2478343.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2481..3545
                     /gene="ada"
                     /locus_tag="N7A07_003760"
     CDS             2481..3545
                     /gene="ada"
                     /locus_tag="N7A07_003760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU2478344.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3545..4195
                     /gene="alkB"
                     /locus_tag="N7A07_003761"
     CDS             3545..4195
                     /gene="alkB"
                     /locus_tag="N7A07_003761"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU2478345.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4271..5914
                     /gene="yojI"
                     /locus_tag="N7A07_003762"
     CDS             4271..5914
                     /gene="yojI"
                     /locus_tag="N7A07_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU2478346.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6132..7778
                     /gene="mqo"
                     /locus_tag="N7A07_003763"
     CDS             6132..7778
                     /gene="mqo"
                     /locus_tag="N7A07_003763"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU2478347.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7928..8416)
                     /gene="eco"
                     /locus_tag="N7A07_003764"
     CDS             complement(7928..8416)
                     /gene="eco"
                     /locus_tag="N7A07_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU2478348.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2015 a         2122 c         2292 g         2008 t
ORIGIN      
        1 aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttgcc
       61 gactgattaa tgagggttaa tcagtatgca gtggcataaa aaagcaaata aaggcatata
      121 acagagggtt aataacatga aagttaaagt actgtccctc ctggtcccag ctctgctggt
      181 agcaggcgca gcaaacgctg ctgaagttta caacaaagac ggcaacaaat tagatctgta
      241 cggtaaagta gacggcctgc actatttctc tgacgacaag tctgtagatg gcgaccagac
      301 ctacatgcgt cttggcttca aaggtgaaac tcaggttact gaccagctga ccggttacgg
      361 ccagtgggaa tatcagatcc agggcaacag cgctgaaaac gaaaacaact cctggacccg
      421 tgtggcattc gcaggtctga aattccagga tgtaggttct ttcgactacg gtcgtaacta
      481 cggcgtagtt tacgacgtaa cttcctggac cgacgttctg ccagaattcg gtggtgacac
      541 ctacggttct gacaacttca tgcagcagcg tggtaacggt ttcgcgacct accgtaacac
      601 tgacttcttc ggtctggttg acggcctgaa ctttgctgtt cagtaccagg gcaaaaacgg
      661 tagcgtaagc ggcgaaggca tgactaacaa cggtcgtgaa gcactgcgtc agaacggcga
      721 cggcgttggc ggatctatca cttatgatta cgaaggcttc ggtatcggtg ctgcagtttc
      781 cagctccaaa cgtactgatg atcagaacag cccgctgtac atcggtaacg gcgaccgtgc
      841 tgaaacctac actggtggtc tgaaatacga cgctaacaac atctatctgg ctgctcagta
      901 cacccagacc tacaacgcaa ctcgcgtagg ttccctgggt tgggcgaaca aagcacagaa
      961 cttcgaagct gttgctcagt accagttcga cttcggtctg cgtccgtccc tggcttacct
     1021 gcagtctaaa ggtaaaaacc tgggtgtcat caatggtcgt aactacgacg acgaagatat
     1081 cctgaaatat gttgatgttg gcgcgaccta ctacttcaac aaaaacatgt ccacctatgt
     1141 tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg gcatcaacac
     1201 tgataacatc gtagctctgg gtctggttta ccagttctaa tctcgattga tatcgaacaa
     1261 agggcctgcg ggcccttttt tcattgtttt cagcgtacaa actcagtttt ttggtgtact
     1321 cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc gcgtggcact
     1381 gctggctgcc gcgctcttct ttgttggttg cgatcaaaaa ccacaacccg ccaaaaccca
     1441 cgctactgaa gttaccgttc ttgaaggcaa aactatgggt accttctggc gtgccagcat
     1501 cccgggcatt gacgccaaac gcagcgccga acttaaagaa aagattcaga cccagctgga
     1561 cgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc gctttaacga
     1621 ctcgcaaagt ttatcgccgt ggccggtaag tgaagcgatg gccgatatcg tcaccacctc
     1681 gctacgcatt ggcgcgaaga ccgatggcgc gatggatata accgtcgggc cgctggtgaa
     1741 tctgtggggt ttcggtccgg aacaacagcc ggttcaaatt ccgagccagg aacagatcga
     1801 cgcgatgaaa gccaaaactg gtttacagca ccttacggtc attaatcagt cgcatcagca
     1861 atatctgcaa aaagacctgc cggatttata tgtcgatctc tccaccgtcg gcgaaggtta
     1921 tgcggcggat cacctggcac gcttgatgga gcaggaaggg atttcccgct atctggtgtc
     1981 ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggcctgccgt ggcgggtagc
     2041 gattcaaaaa ccaaccgata aagaaaacgc ggttcaggcc gtggtggata tcaacggtca
     2101 tgggattagc acctctggca gctaccgtaa ctattacgaa ctggacggca aacgtctttc
     2161 ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac aatctggtat ccgtgacggt
     2221 gattgcaccg acggcgctgg aagccgacgc ctgggataca ggtttgatgg tactcgggcc
     2281 ggagaaagcc aaagaagttg ttcgccggga agggctggcg gtttatatga tcaccaaaga
     2341 aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg tcagcgaaaa
     2401 aaattaaagc gcaagattgt tggtttttgc gtgatggtgg ccgggcagac taaaggctat
     2461 ccttaaccag ggagctgatt atgaaaaacg ccacatgctt aactgacgat caacgctggc
     2521 aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc gtgcgcacca
     2581 caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa aacgtctcct
     2641 tctacgcaaa tgccagcgag gcactcgccg ccggatttcg cccctgcaaa cgctgccaac
     2701 ctgataaagc caatgcccag caacatcggt tggataaaat cacccacgcg tgtcgactgc
     2761 tggaacagga aacgcctgta acgttggaag ccttagccga ccaggtggcg atgagtccat
     2821 ttcatctaca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc tggcaacagg
     2881 cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg acgacgtcta
     2941 ttcttaacgc cggattcccc gacagcagca gttactacca caaagccgat gaaacgctgg
     3001 gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt tacgcgctgg
     3061 ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt tgcgcgatat
     3121 tgctgggcga tgatgacgcg acattaatca gcgagttgca gcagatgttt cccgctgccg
     3181 acaacgcgcc tgccgatctg acgtttcagc aacatgtgcg tgaagtgatc gccagcctca
     3241 atcaacgcga tactccgctg acgttaccgc tggatattcg cggcactgct tttcagcaac
     3301 aagtctggca ggcactgcgc acgatacctt gcggtgaaac cgtcagttat cagcaactgg
     3361 ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt gccgccaaca
     3421 agctggctat cgtaattccc tgtcatcgag tggtccgtgg tgatggcaca ctttccggtt
     3481 accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa aatgaggaga
     3541 ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg cggctggtgc
     3601 ggtaatttta cggcgttttg cttttaacgc tgcagagcaa ctaatccgcg atattaatga
     3661 cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc gggggatata ccatgtcggt
     3721 ggcgatgacc aactgtgggc atctgggctg gacgacccat cggcaaggtt atctctattc
     3781 gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg ccgcagagtt ttcataattt
     3841 atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg cttgtcttat
     3901 caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg aaccggatct
     3961 gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat ttggtggcct
     4021 gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg tggtatgggg
     4081 cggtgaatcg cggctgtttt atcacggtat tcaaccgttg aaagcggggt ttcatccact
     4141 caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag aataaaaata
     4201 agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat tcattgtccg
     4261 ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc catttatcag
     4321 tgtgatggcg ctaagcctcg ccagtgcggc tttaggcatt ggcttaattg cttttatcaa
     4381 tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttgccggagt ttctgggatt
     4441 attgctgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca ctttggggca
     4501 tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata ctcacgtcga
     4561 gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg atgtgcgcaa
     4621 tatcaccatt gcttttgtgc gtctgccgga actggtgcag gggatcattc tcactatcgg
     4681 ttcagcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg ctatctggat
     4741 ggcagtcacc atctggggcg gttttgtgct ggtggcgcgg gtgtacaaac atatggcgac
     4801 cctgcgtgaa accgaagaca agctgtacac ggattttcaa actgtacttg aagggcgcaa
     4861 agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct acattcctga
     4921 tgcgcaagag tatcgccatc atattattcg cgcagacacc ttccatctta gtgccgtgaa
     4981 ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg cgaacagtct
     5041 cggttgggct gataccaacg ttgccgcgac ctattcgttg acgcttttat tcctgcgtac
     5101 gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg tggcgtttaa
     5161 caagctgaac aaattcgcgc tcgcgccttt caaagcagag tttccgcgcc cgcaggcgtt
     5221 tcccagctgg caaacgctgg agctgcgtaa cgtgacgttt gcttatcagg ataacgcgtt
     5281 ttccgttggt ccgattaatc tcaccatcaa acgtggcgag ctgctgtttc tgattggcgg
     5341 caacggtagc ggaaaatcga cgctggcgat gttgttgacg ggcttgtatc agccacaaag
     5401 cggcgaaatc ttgctggatg gcaaacctgt cagcggcgaa caaccggaag attatcgcaa
     5461 actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg ggccggaggg
     5521 taaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa tggctcataa
     5581 gcttgagtta agaaacgggc gtattgttaa cctgaagtta tcaaaagggc agaaaaaacg
     5641 cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg atgaatgggc
     5701 ggcggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc cgctgatgca
     5761 ggagatgggt aaaactattt tcgccatcag tcatgatgat cattacttta tccacgccga
     5821 ccgcctgctg gaaatgcgca acgggcaact tagcgagctg acgggcgaag agcgcgatgc
     5881 cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc tggcggactg
     5941 ctgccgccag gtcaatatgc taaaaatccc ttcatttcta ataaccctat aattaatcaa
     6001 cgaaattata atgtttctaa aattagaata taatttataa acattattta aatgttgtta
     6061 cttaagtgtt accgttgatg ccgcgcaaat ctcacctgca ataaagcgac taaaagtaag
     6121 gcattaacaa gatgaaaaaa gtgactgcca tgctcttctc gatggccgtg gggcttaatg
     6181 ccgtttcgat ggcggcaaaa gcgaaagcgt ccgaggagca ggaaactgat gtactgttga
     6241 ttggcggcgg cattatgagc gccacgttgg ggacctattt acgcgagctg gagcctgaat
     6301 ggtcgatgac catggtggag cgcctggagg gtgtcgcgca ggagagttcg aacggctgga
     6361 ataacgccgg aaccgggcat tctgcactga tggaactgaa ctacaccccg caaaacgccg
     6421 atggcagcat cagtattgaa aaagcagtcg ccattaacga agcatttcag atttcccgcc
     6481 agttctgggc gcaccaggtc gagcgcggcg tgctgcgtac tccgcgttca tttatcaata
     6541 ccgttccgca tatgagcttt gtctggggcg aggataacgt caatttcctg cgcgcccgtt
     6601 acgccgcgtt gcaacaaagc tcgctgtttc gcggtatgcg ttactctgaa gatcacgcgc
     6661 agatcaaaga gtgggcaccg ttagtgatgg aagggcgcga tccgcaacag aaagtggcag
     6721 ccacgcgtac ggaaattggt accgatgtga actacggcga gatcacccgc cagttaattg
     6781 cttccttgca gaagaaatct aacttctcgc tgcaactcag cagcgaagtc cgcgccctaa
     6841 agcgtaatga cgataacacc tggaccgtta ccgttgccga tctgaaaaat ggcactgcac
     6901 agaacatccg tgcgaaattt gtctttatcg gcgcgggcgg tgcggcactg aagctgttac
     6961 aggaatcggg gattccggaa gcgaaagact acgccggttt cccggtgggc ggacagttcc
     7021 ttgtttcgga aaacccggac gtggttaatc accatctggc gaaggtttac ggtaaagcat
     7081 ccgttggcgc accaccgatg tcggttccgc atatcgatac ccgcgttctg gacggtaaac
     7141 gcgtagtgct gtttgggcca tttgccacct tctcaaccaa attcctcaaa aacggttcat
     7201 tgtgggatct gatgagttcc accaccacct ctaacgtgat gccgatgatg cacgtcgggc
     7261 tggataattt cgatctggtg aaatatctgg tgagtcaggt gatgttgagt gaagaggatc
     7321 gttttgaagc gttgaaagag tactatccgc aagcgaaaaa agaggactgg cgtttgtggc
     7381 aagcggggca gcgtgtgcag attatcaagc gtgatgccga taaaggtggt gtactgcgtc
     7441 tgggtactga agtggtcagt gaccagcaag gaaccattgc cgcgctcctg ggggcatcgc
     7501 caggggcgtc aaccgccgcg ccgattatgt tgaatctgct ggaaaaagta tttggcgatc
     7561 gcgtttccag cccgcaatgg caggctacgt tgaaagcgat cgttccatct tatggacgca
     7621 agctgaacgg tgatgtagcg gcaacagaac gcgagttgca gtacaccagc gaagtgctgg
     7681 ggctgaaata cgacaaaccg caagcagcag atagtacgcc gaaaccgcag ttgaaaccgc
     7741 aacccgttca aaaagaagtg gcggatattg cgttgtaatg atgtgccaca tccggcatgg
     7801 tatgccggat gtggcgtatg ctgataagac gcgccagcgt cgcatcaggc aaccggctcg
     7861 ggcgttagat gtcggatgcg gcgtgaccgc tttatccaac ctacggggtg ccgcacttca
     7921 cggcagttta gcgaactacc gcgttgtcaa ttttctcttc cgccttccag acgcggtact
     7981 tcacatctac attgtctggc gtatacacca cgatcggcag cttgctgttg taacgcagca
     8041 ttccagtatc gcccagatac gcggtgacaa atttcttctc tttcttgcca tccgggcagg
     8101 ccatcatcgt ggaaaccggc gagctaactt tatcaaagac ataataatca tagccccagc
     8161 cttccagcgt tttgctttcc agcttcccgc cgagacgatg caaattgcaa tcgacttcca
     8221 gcgtctgacc gattaacagt tctactttca gggtagattc atcttcttgc ggggttaact
     8281 gaatcacctg acgcttcatg cctttttcag cttgtggata aggcgcgatt ttttccagtg
     8341 gctggacgct ctctgccgcc caggcggaag tggtggcgaa agcggcaaac aatactgcag
     8401 gtagaatggt cttcattttt tgctttcctt cttcatt
//
LOCUS       DBFIFS010000188         8427 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.188, whole genome shotgun sequence.
ACCESSION   DBFIFS010000188 DBFIFS010000000
VERSION     DBFIFS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.188"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..394)
                     /locus_tag="N7A07_003765"
     CDS             complement(<1..394)
                     /locus_tag="N7A07_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU2478349.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRG"
     gene            541..816
                     /gene="yahO"
                     /locus_tag="N7A07_003766"
     CDS             541..816
                     /gene="yahO"
                     /locus_tag="N7A07_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU2478350.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(914..2500)
                     /gene="prpR"
                     /locus_tag="N7A07_003767"
     CDS             complement(914..2500)
                     /gene="prpR"
                     /locus_tag="N7A07_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU2478351.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            2739..3629
                     /gene="prpB"
                     /locus_tag="N7A07_003768"
     CDS             2739..3629
                     /gene="prpB"
                     /locus_tag="N7A07_003768"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU2478352.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            3785..4954
                     /gene="prpC"
                     /locus_tag="N7A07_003769"
     CDS             3785..4954
                     /gene="prpC"
                     /locus_tag="N7A07_003769"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU2478353.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            4988..6439
                     /gene="prpD"
                     /locus_tag="N7A07_003770"
     CDS             4988..6439
                     /gene="prpD"
                     /locus_tag="N7A07_003770"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU2478354.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            6479..8365
                     /gene="prpE"
                     /locus_tag="N7A07_003771"
     CDS             6479..8365
                     /gene="prpE"
                     /locus_tag="N7A07_003771"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU2478355.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         1978 a         2250 c         2337 g         1862 t
ORIGIN      
        1 atccacggcg aaaaaagaca taccaggggg cgttaatcgg ttgttgtagt gtgctcattt
       61 gcggtgttga ctggcggcgc atgctgcacc acgcaaacca taagagataa gcgccgccga
      121 cgattctgat aagcgaaaaa atctcctcac actgcgtaat tagcgttgca agaccaaaca
      181 aacccaaccc ggaataaaat gcatcgccca gcgccacgcc cagtccggtc agcactcctg
      241 cgcgtcgacc ggaagccagg ctggtttgta ctaccacaaa aagattggct cccggattaa
      301 aaaaagtaat cacgaacagt cctacggtca ggtaaacggc atgcaaagga tccatagtga
      361 tttcatccat aaataagtga actaactgca tcatatttct accaaaaata atcgggtgcg
      421 agagagatca caaagtgtct tatttccggt tactggcgtt tatgccctga ctgaactaat
      481 tattaatcaa cccaataatg tcggtgggtg atagtgtgat aacaactctg gagccgtaat
      541 atgaaaataa tctctaaaat gttagtcggt gcgttagcgt ttgccgttac caatgtctat
      601 gccgctgaat tgatgaccaa agcggaattt gaaaaagttg aatcgcagta tgaaaaaata
      661 ggtgatattt caaccagtaa tgaaatgtcg actgcagatg caaaagaaga tttaatcaaa
      721 aaagccgatg aaaaaggggc tgatgtgttg gtactgacct ccggtcaaac tgacaataag
      781 atccacggca cggcggatat ttataagaag aagtaattct gaatcctatg taaacatccc
      841 cgatgcgtaa gtttatcggt gatcatctat tgaaatttat gccggataaa gcgttcgcgc
      901 tgcattcggc agttcagctt ttcagccgcc gccagaacgt cgtccggctg atgcctaaat
      961 aattcgccgc tgctgtttta tcgccattaa atttctccag tgcctgttgt ggtgtcagca
     1021 agcgtggagc gggaattttc gccgactcgc gcgccagttc cggcagtagc agctgcaaaa
     1081 actgcggcgt taaatccggc gtcggttcca cacttaaaaa tagcgccagt cgctccatca
     1141 tattgcgcag ttcacgaata ttacctggcc agtcgtagtg caccagcacg gtttcgcttg
     1201 cctgtaatcc ctggcgtaat gcggcagaaa atggggcgga gagcgccgcc agagacactt
     1261 tcaaaaagct ttccgccagc ggaagaatat ctgccacccg ctcgcgcagt ggtggcaatt
     1321 gcagacgcaa aatactcagc cgataaaaca gatcacggcg aaactgccct tgccgcatat
     1381 cttcttccag attgcagtga gtggcgctaa tgacccgtac atctaccgga acaggctgat
     1441 gcccgccgac gcgggtgacc tctttttctt ccagcacccg taacaggcga gtctgcaaag
     1501 gtagcggcat ttcgccaatc tcatccagaa acagcgtgcc accgtgggca atttcgaaca
     1561 gcccggcgcg acctccgcgt cgcgagccgg taaacgcccc ttcctcatag ccaaacagct
     1621 ccgcttccag cagcgattcg gcaatcgccc cgcagttgac ggcaacaaac ggatgcgact
     1681 ttttgccctg tcgcgcatcg tggcgggcaa aatattcccg atgaattgcc tgggccgcca
     1741 gctctttgcc cgtccccgtt tccccctcaa tcaacaccgc cgcactggag cgggcataca
     1801 gcaaaatagt ctgccgcacc tgctccatct gtggtgattg accgagcata tcgcccagca
     1861 cgtaacgagt acgcagggcg ttgcgggtgg catcgtgagt gttatggcgt aacgacatgc
     1921 gcgtcatatc cagcgcatcg ctgaacgcct ggcgcacggt ggcggcggaa tagataaaaa
     1981 ttccggtcat tccggcttct tctgccagat cggtaatcag ccccgcgccg accaccgctt
     2041 cggtgccgtt agcttttagc tcgttaatct gcccgcgtgc gtcttcttcg gtaatgtagc
     2101 tacgttgatc gaggcgcaaa ttaaaggttt tttgaaacgc caccagtgcc ggaatagttt
     2161 cctgataagt gacaacgccg atagaagagg tgagttttcc ggcttttgcc agtgcctgta
     2221 acacatcgta gccgctcggt ttaatcaaaa taactggcac tgacaggcgg cttttcaggt
     2281 acgcgccgtt agagccagcg gcgatgatgg cgtcacagcg ttcgtttgcc agtttcttgc
     2341 ggatgtaggt cactgctttt tcaaagccaa gctgaatagg ggtaatgttc gccaggtgat
     2401 caaactcgag gctgatatcg cgaaacagct cgaataagcg cgttacagat accgtccaga
     2461 taaccggttt gtcgtcattc agccgtggtg gatgtgccat agcgcaccgc aaagttaaga
     2521 taccgaatat tgggtttagt cttgtttcat aattgttgca atgaaacgcg gtgaaacatt
     2581 gcctgaaacg ttaactgaaa cgcatatttg cggattagtt catgacttta tctctaacaa
     2641 attgaaatta aagatttaat tttattaagg caattgtggc acaccccttg ctttgtcgtt
     2701 atcaacgaaa ataacaagtt gataacaaag gatgggctat gtctctacac tctccaggta
     2761 aagcgtttcg cgctgcactg actaaagaaa atccattgca gattgttggc accattaacg
     2821 ccaaccatgc gctgttggcg cagcgtgccg gatatcaggc aatttatctc tccggcggtg
     2881 gcgtggcggc agggtcgctg gggctgcccg atctcggtat ttctactctt gatgacgtgt
     2941 tgacagatat tcgccgtatc accgacgttt gttcgctgcc gctgctggtg gatgcggata
     3001 tcggttttgg ttcttctgct tttaacgttg cgcgtacggt gaaatcgatg attaaagccg
     3061 gtgcggcggg attgcatatt gaagatcagg ttggtgcaaa acgctgtggt catcgcccga
     3121 acaaagcgat cgtctcgaaa gaggagatgg tggatcggat ccgcgcggcg gtggatgcga
     3181 aaaccgatcc tgattttgtg atcatggcgc gcaccgatgc gctggcggta gaggggctgg
     3241 atgcggcgat cgagcgtgcg caggcctatg ttgaagcggg tgccgagatg ttgttcccgg
     3301 aggcgattac cgaactcgcc atgtaccgcc agtttgccga tgcggtgcag gtgccaatcc
     3361 tcgccaacat taccgaattt ggcgccacgc cgctgtttac caccgacgaa ttacgcagcg
     3421 ctcatgtcgc gatggcgctg tacccgcttt cagcgtttcg cgccatgaac cgcgccgccg
     3481 aacatgtcta caacgtcctg cgccaggaag gcacgcagaa aagcgtcatc gacaccatgc
     3541 agacccgcaa cgagctgtac gaaagcatca actactacca ctacgaagag aagctcgacg
     3601 acctgtttgt ccgtagccag gcgaaataaa aaacgcccgt tggttgtatt cgacaaccga
     3661 tgcctgatgc gccgctgacg cgacttatca ggcctacggg gtgaactgta ggtcggataa
     3721 gacgcatggc gtcgcatccg acaacaatct cgaccctaca aatgataaca atgacgagga
     3781 caatatgagc gacacaacga tcctgcaaaa cagtacccat gtcattaaac cgaaaaaatc
     3841 tgtggcgctt tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg gtaaaagtgg
     3901 caatgacttg cattaccgtg gctacgatat tcttgatctg gcggaacatt gcgaatttga
     3961 agaagtggcg catctgctga tccacggcaa actgccgacc cgtgacgaac tcgccgccta
     4021 caaaacgaaa ctgaaagccc tgcgcggttt accggctaac gtgcgtaccg tgctggaagc
     4081 cttaccggcg gcgtcacacc cgatggatgt tatgcgcacc ggcgtttccg cgctcggctg
     4141 cacgctgcca gaaaaagagg ggcataccgt ctctggcgcg cgggatattg ccgacaaact
     4201 gctggcgtca cttagctcga ttcttctcta ctggtatcac tacagccaca acggcgaacg
     4261 catccagccg gaaaccgatg acgactccat cggtggtcac ttcctgcatc tgctgcacgg
     4321 cgaaaagccg tcgcaaagct gggaaaaggc gatgcatatt tcgctggtgc tgtacgccga
     4381 acacgagttt aacgcctcca cctttaccag ccgggtgatt gcgggcactg gctctgatat
     4441 gtattccgcg attattggcg cgattggtgc actgcgcggg ccgaagcacg gcggggcgaa
     4501 tgaagtgtcg ctggagatcc agcaacgcta cgaaacgccg gacgaagccg aagccgatat
     4561 ccgcaagcgg gtggaaagca aagaagtggt cattggtttt ggtcatccgg tttataccat
     4621 cgccgacccg cgccaccagg tgattaaacg tgtggcgaag cagctctcgc aggaaggcgg
     4681 ctcgctgaag atgtacaaca tcgccgatcg cctggaaacg gtgatgtggg agagcaaaaa
     4741 gatgttcccc aatctcgact ggttctccgc tgtttcctac aacatgatgg gcgttcccac
     4801 cgagatgttc acaccactgt ttgttatcgc ccgcgtcacc ggctgggcgg cgcacattat
     4861 cgaacaacgt caggacaaca aaattatccg tccttccgcc aattatgttg gaccggaaga
     4921 ccgccagttt gtcgcgctgg ataagcgcca gtaaacctct acgaataaca ataaggaaac
     4981 gtacccaatg tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg aaatcgttga
     5041 tatcgtcgat tacgtcatga actacgaaat cagctccaaa gtagcctacg acaccgcaca
     5101 ttactgtctg ctcgacacgc tcggctgcgg tcttgaagct ctcgaatacc cagcctgtaa
     5161 aaaactgctg gggccaattg tccccggcac cgtcgtacct aacggcgtgc gcgtccccgg
     5221 aactcagttc cagctcgacc ccgtccaggc ggcatttaac atcggcgcga tgatccgctg
     5281 gctcgatttc aacgatacct ggcttgcggc agagtggggc catccttccg acaatctcgg
     5341 cggcattctg gcaacggcgg actggctttc acgcaacgcg gtcgccagcg gcaaagcgcc
     5401 gttgaccatg aaacaggtgc tgaccggaat gattaaagcc catgaaattc agggctgcat
     5461 cgcgctggaa aactccttta accgcgtcgg cctcgaccac gttctgttag tgaaagtggc
     5521 ttccaccgcc gtggtcgccg aaatgcttgg cctgacccgc gaggaaattc tcaacgctgt
     5581 ttcgctggcg tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc cgaacaccgg
     5641 cacgcgtaaa tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc tggcactgat
     5701 ggcgaaaacg ggcgaaatgg gttacccgtc agccctgact gcgcctgtgt ggggcttcta
     5761 cgacgtctcc tttaaaggtg aatcgttccg cttccagcgt ccgtacggtt cttacgtgat
     5821 ggaaaatgtg ctgttcaaaa tctccttccc ggcggagttc cactcccaga cggcagttga
     5881 agcagcgatg acgctctatg aacagatgca ggcagcaggc aaaacggcgg cagatatcga
     5941 aaaagtgtcc atccgcaccc acgaagcctg tattcgcatc atcgacaaaa aggggccgct
     6001 caataacccg gcagaccgcg accactgcat tcagtacatg gtggcgatcc cactgctatt
     6061 cgggcgctta acggcggcag attacgagga caacgttgcg caagataaac gcattgacgc
     6121 cctgcgcgag aagatcaatt gctttgaaga tccggtattt accgctgact accacgaccc
     6181 ggaaaaacgc gccatcgcca atgccattac ccttgagttc accgacggca cacgatttga
     6241 agaagtggtg gtggagtacc ccattggtca tgctcgccgc cgtcaggatg gtattccgaa
     6301 actggtcgat aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc aacagcagcg
     6361 cattctggag gtttctctcg acagagctcg cctggaacag atgccggtca atgagtatct
     6421 cgacctgtac gtcatttaag taaacggcgg taaggcgtaa gttcaacagg agagcattat
     6481 gtcttttagc gaattttatc agcgttcgat taacgaaccg gagcagttct gggccgagca
     6541 ggcccggcgt attgactggc agacgccctt tacgcaaacg ctcgatcaca gcaatccgcc
     6601 gtttgcccgt tggttttgtg aaggccgaac caacttgtgc cacaacgcta tcgaccgctg
     6661 gctggagaaa caaccagagg cgctggcgct gattgccgtc tcttcggaaa cagaagaaga
     6721 gcgcaccttt acctttcgcc agcttcatga cgaagtaaac gcggtggcgt caatgctgcg
     6781 ctcgctgggc gtgcagcgtg gcgatcgggt gctggtgtat atgccgatga ttgccgaagc
     6841 gcatattact ctgctggcct gcgcgcgcat tggcgctatt cactcggtgg tgtttggggg
     6901 atttgcctcg cacagcgtgg cggcgcgaat tgatgatgct aaaccggtgc tgattgtctc
     6961 ggctgatgcc ggagcgcgcg gtggcaaaat cattccctat aaaaaattgc tcgacgatgc
     7021 gataagtcag gcgcagcacc agccacgcca tgttttgctg gtggatcgcg ggctggcgaa
     7081 aatggcacgc gtcagcgggc gggatgtcga tttcgcgtcg ttgcgccatc aacacatcgg
     7141 cgcgcgggta ccggtggcgt ggctggaatc caacgaaacc tcctgcattc tctacacctc
     7201 cggcacgacc ggcaaaccta aaggcgtgca gcgtgacgtt ggcggatatg cggtggcgct
     7261 ggcgacctcg atggacacca tttttggcgg caaagcgggc ggtgtgttct tttgtgcttc
     7321 ggatatcggc tgggtggtgg ggcattcgta tatcgtctac gcgccgctgc tggcggggat
     7381 ggcgactatc gtttacgaag gattaccgac ctggccggac tgcggcgtgt ggtggaaaat
     7441 cgtcgagaaa tatcaggtta gccggatgtt ctcagcgccg accgccattc gcgtgctgaa
     7501 aaaattccct accgctgaaa ttcgcaaaca cgatctctcg tcgctggaag tgctctatct
     7561 ggctggagaa ccgctggacg agccgaccgc cagttgggtg agcaatacgc tggatgtgcc
     7621 ggtcatcgac aactactggc agaccgaatc cggctggccg attatggcga ttgctcgcgg
     7681 tctggacgac aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg gctataacgt
     7741 gcagttgctt aatgaagtca ccggcgaacc gtgtggcgtc aacgagaaag ggatgctggt
     7801 ggtggaaggg ccgctgccgc cggggtgtat tcagaccatc tggggcgacg acgaccgctt
     7861 tgtgaagacg tactggtcgc tgttttcccg cccggtgtac gccacctttg actggggcat
     7921 ccgtgacgct gacggttatc actttattct cgggcgcact gacgatgtga ttaacgttgc
     7981 cgggcatcgg ctggggacgc gcgagattga agagagtatc tccagtcatc cgggcgttgc
     8041 cgaagtggcg gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg tggcgtttgt
     8101 cattccgaaa gagagcgaca gtctggaaga tcgtgatgtg gcgcattcgc aagagaaggc
     8161 gattatggcg ctggtggaca gccagattgg caactttggc cgcccggcgc acgtctggtt
     8221 tgtctcgcaa ttgccaaaaa cgcgatccgg aaaaatgctg cgccgcacga tccaggcgat
     8281 ttgcgaagga cgcgatcctg gggatctgac gaccattgat gatcctgcgt cgttgaatca
     8341 gatccgccag gcgatggaag agtaaggtat tgtcggatgc gtcgcgtggc gcatccgaca
     8401 gttgtgcgca gatgcctgat gcgacgc
//
LOCUS       DBFIFS010000189         8248 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.189, whole genome shotgun sequence.
ACCESSION   DBFIFS010000189 DBFIFS010000000
VERSION     DBFIFS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.189"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            289..2526
                     /gene="nrfB"
                     /locus_tag="N7A07_003772"
     CDS             289..2526
                     /gene="nrfB"
                     /locus_tag="N7A07_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU2478356.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2513..5485
                     /gene="nfrA"
                     /locus_tag="N7A07_003773"
     CDS             2513..5485
                     /gene="nfrA"
                     /locus_tag="N7A07_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU2478357.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5486..6376
                     /locus_tag="N7A07_003774"
     CDS             5486..6376
                     /locus_tag="N7A07_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU2478358.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6559..7320
                     /gene="envY"
                     /locus_tag="N7A07_003775"
     CDS             6559..7320
                     /gene="envY"
                     /locus_tag="N7A07_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU2478359.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7366..7442)
                     /locus_tag="N7A07_003776"
     tRNA            complement(7366..7442)
                     /locus_tag="N7A07_003776"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7406..7408),aa:Arg,seq:tct)
     gene            7685..>8248
                     /gene="fimZ"
                     /locus_tag="N7A07_003777"
     CDS             7685..>8248
                     /gene="fimZ"
                     /locus_tag="N7A07_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU2478360.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNI"
BASE COUNT         2056 a         2126 c         2096 g         1970 t
ORIGIN      
        1 ttgcggggag cgggctttcg taatcttgcc ctgcctgctc acctccaggc acttccacta
       61 catcatgacc tgatcggtgc caccactcct cttcactatg acgttaacct cccctccttg
      121 acaccacgct tttgtctaca ttctacgcaa acggcaactt ttataaccag tgtaaaaata
      181 acgtgccgca atagtcatac ctattaatgg taaaaagctg tcacaattca taaaaaacct
      241 taatatacgc caccctaaac ataaccagcg ttaatgtaag gtttttgtgt ggactggctt
      301 cttgatgttt ttgctacctg gctctatggc ttaaaagtaa tcgcgataac gttagcggtc
      361 atcatgttca tcagcgggct ggacgatttt tttattgatg tcgtctactg ggtacgccgc
      421 attaaacgca agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat
      481 aaaccagatg aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac gggcgtcatc
      541 ggcaatatgg ccgagctggc ggcgaccacg ctcgactacg aaaactatca tatctttgtt
      601 ggcacctacc ccaacgaccc ggacactcag cgtgatgttg acgaagtgtg cgctcgcttc
      661 ccgaatgtgc ataaggtagt ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg
      721 aacaacgtgc tggacgccat cacccagttt gagcgtagcg ccaatttcgc ttttgctggt
      781 tttattctgc atgacgccga agatgtgatt tcaccgatgg aattgcgtct gttcaactat
      841 ctggtcgagc gtaaagatct gattcagatc ccggtgtatc cgtttgaacg cgaatggacg
      901 cacttcacca gcatgactta cattgatgag ttttcagagc tgcatggcaa agatgttccg
      961 gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg tttcagccgc
     1021 cgcgccgtga ccgctctgtt agctgacggt gacggtattg ctttcgacgt gcagagtctg
     1081 actgaagatt acgacattgg cttccgcctg aaagaaaaag gtatgacgga aatttttgtc
     1141 cgttttccgg tggtggacga agccaaagaa cgcgagcagc gtaaattttt acagcacgca
     1201 cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg ataccttttc gactgcggtt
     1261 cgacaaaaat ctcgctggat catcggcatt gttttccagg gctttaaaac ccataaatgg
     1321 acctccagcc tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc aatcagtaac
     1381 tttgtcagct tcctcgcaat gctggtgatg atccagcttt tgctgttgct ggcgtatgaa
     1441 agtttgtggc ccgatgcctg gcatttcctt tctattttta gcggcagcgc atggttaatg
     1501 accctgctgt ggctaaactt tggcttgatg gttaaccgca tcgtgcagcg ggtgattttc
     1561 gtcactggct actacggcct gacgcagggg ctactatctg tcctgcgtct tttctggggc
     1621 aacctgatta actttatggc caactggcgc gcgttaaaac aggtacttca acacggcgat
     1681 ccacgtcgcg tcgcgtggga taaaacaacg catgacttcc ccagcgtcac tggcgatacc
     1741 cgctcgttgc gcccgttagg tcaaattctg ctggaaaatc aggtcatcac tgaagaacag
     1801 ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg
     1861 caggggctga ttagcgccga gcagctggca caggcgctgg cagagcaaaa cggcgtggcg
     1921 tgggaatcca tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat gccggcctcc
     1981 gtggcgctgc attatgcggt actgccgctg cgtctggaaa atgatgagtt aattgtcggc
     2041 agtgaagatg gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa
     2101 gtgcgttatg tcattgttct gcggggacaa attgtcacgg ggttacgtca ctggtatgca
     2161 cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca tcagtggctc
     2221 acggaacagc aggccggtga aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc
     2281 gaaatactga ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc
     2341 catgaacgca gttctctgcc gctcggcaag tttttggtca ccgaaggcgt tatcagccag
     2401 gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac aagtttcgat gcaatcacta
     2461 ttactcaaag caggtttaaa cacagaacag gttgcgcaac tggagtccga aaatgaagga
     2521 gaataacctt aatcgcgtca tcggatggtc tggtttactg ctgacgtctt tattgagtac
     2581 cagcgcactc gcagacaata tcggcaccag cgcagaagag ctggggctga gcgattatcg
     2641 ccattttgtt atttatcccc gtctcgataa ggcgctgaag gcacagaaaa ataacgacga
     2701 agcaaccgcc atccgcgaat ttgaatatat acaccagcag gtgccggata atattccgct
     2761 gactttatac cttgcggaag cctatcgcca ttttggtcat gatgaccggg cacggctgtt
     2821 gcttgaggat caactgaaac gtcacccagg agatgcccga cttgagcgca gtctggcggc
     2881 tattccggtt gaagtgaaaa gcattacaac tgttgaagaa ctgcttgccc aacaaaaagc
     2941 gtgcgatgct gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct
     3001 ggcacagtta cctgtcgcca gagcgcaact gaacgacgcg acgtttgctg catcgccgga
     3061 aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc tacctgaaac aatggtccca
     3121 ggcagatacg ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg
     3181 tcagtggttt gacgtgcttc ttgccgggca gctggacgat cggatcctgg cactgcaatc
     3241 acaggggatc ttcaccgatc ctcagtcata tattacttac gcgaccgcgc tggcttatcg
     3301 tggcgaaaaa gcacgcctcc agcattatct cattgaaaat aagccgctgt ttaccacgga
     3361 cgcacaagag aaaagttggc tctatctgtt atctaaatac agcgccaacc ccgttcaggc
     3421 attggcgaat tatacggtgc agtttgccga taaccgccag tatgttgttg gcgcgacgct
     3481 accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc
     3541 cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta acaaggctga
     3601 agctctgcgt ctggcacgct tgctgtatca gcaagaatcg gcaaatctta cccgcctgga
     3661 tcaactaacc tggcaactga tgcagaacga gcagtcacgg gaagctgccg atttattgct
     3721 gcaacgctat cctttccagg gcgatgcgcg tatcagccag actttaatgg cgcgactggc
     3781 gtctctgctg gaaagtcatc cttacctggc aacgccggcg aaggtggcga ttttatcgaa
     3841 acccttaccg ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa
     3901 ttgcccggca atagttcgct tgctgggcga tatgtcgcct tcctacgatg ccgccgcctg
     3961 gaaccgtctg gcaaagtgtt atcgggacac gctacccggt gtggcgttgt atgcatggct
     4021 tcaggccgaa caacgacaac cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta
     4081 tcaggttgag gactacgcca ccgcactggc ggccaggcag aaaatcagtc ttcacgacat
     4141 gagcaatgag gatctgcttg ctgctgccaa taccgcccag gcggcaggaa atggtgcggc
     4201 tcgcgatcgc tggctacaac aggcagaaca acgtggactg ggaagcaatg ccctctactg
     4261 gtggctgcat gcgcaacgtt acattcctgg tcagccggaa ctcgcactga acgatctcac
     4321 gcgctcaatc aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga caatttatcg
     4381 ccaacgtcat aatgtcccgg ccgcggtgag tgatttgcgc gccgcgctgg aactggaacc
     4441 gaataatagc aacacccagg cagcgcttgg ttacgccttg tgggatagcg gtgatatcgc
     4501 acagtcgcgg gaaatgctcg aacaggcgca taaagggcta ccggacgatc cggcactgat
     4561 ccgacaactg gcctatgtga accagcgtct ggatgacatg cctgcgacgc agcactacgc
     4621 ccggctggtg attgatgaca ttgataatca ggcgctgata accccactga ccccagagca
     4681 aaatcagcaa cgcttcaatt tccgccgtct gcatgaggag gtcggtcgcc gctggacgtt
     4741 cagtttcgat tcttccatcg gcttgcgttc cggggcaatg agtaccgcta acaataatgt
     4801 tggcggcgca gcgccaggga aaagctatcg tagctacggg caactggaag ccgaataccg
     4861 catcggacgc aatatgctgc tggaaggcga cctgctctca gtttatagcc gcttctttgc
     4921 cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct
     4981 gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc agttgccgct
     5041 gaacgggcaa aatggcgcat ccgataccat gctgcgcgcc agcgcctcat tctttaatgg
     5101 cggcaaatac agcgacgaat ggcacccgaa cggttcaggc tggtttgccc aaaacctgta
     5161 cctcgatgcg gcgcaatata tccgccagga tattcaggcg tggacggcag attatcgcgt
     5221 cagctggcat cagaaggtag ctaacggaca gactattgag ccttacgctc acgttcagga
     5281 caacggctat cgtgataaag gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg
     5341 gaatatctgg accggcgaga cgcactacga cgcctggccg cacaaagtca gtctcggcgt
     5401 cgagtatcaa cataccttta aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt
     5461 tctcaccatt ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc tgacactgct
     5521 tttggtcagc cctttttcct ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga
     5581 tagtcaggtt accgataccc agtggcaggg gctgatgagt cagttacgtt tgcaaggctt
     5641 cgataccctg gttttgcaat ggacccgtta cggcgatgca ttcacccagc cagaacagcg
     5701 cgcgttattg tttaagcggg ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg
     5761 gctgaacgcc gatccggaat tttttatgca ccagaaacag tcatccgcgg cgctggaaag
     5821 ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc agattatgga gtgccgtacc
     5881 tggcgtaacg ccggatggct ggtacttcag cgctgaaatt gacgacctga actggcgcag
     5941 cgaagccgcc cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc tgattagcga
     6001 tgtttcagca aaaccggttt atatcagtag ttttttcgcc ggaaacatgt cgccagatgg
     6061 ctatcgccaa ctgctggaac acgttaaagc aaccggcgtt aatgtctggg tacaggatgg
     6121 cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga
     6181 ttgccaaagc tccgccccag caagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg
     6241 caaaaccttt acagcgaaac cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg
     6301 ttcctcttgc ggtaaagaca ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg
     6361 cattctcgag tattaaatct cctccaggta agtcgggtac gacctggctt acctctttcg
     6421 ctctcgtcag aattccatca agattaattg ctaaaaagcg tgctaaagaa agaagatcgt
     6481 tatcggcgaa cgggccgatc actcatgcag gaagcataga tcctttgagc ggagtttctg
     6541 atatctgagg agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt gatcctgacc
     6601 gaaaaagagg tagaggtaag cgtcaataac catgctacgt ttacccttcc gaaaaactac
     6661 ctggccgcct tcgcgtgcaa caataacgtc attgaactct caacgttaaa tcacgtatta
     6721 atcacccaca tcaaccgtaa catcatcaac gattatctgt tgtttttaaa taagaactta
     6781 acctgtgtaa agccctggtc gcggctggct accccggtta tcgcttgtca tagccgtaca
     6841 ccggaagtgt tccggctagc cgccaaccac agcaagcagc aacccagcaa accctgcgaa
     6901 gcggagttga cgcgcgcatt gctttttacc gtattgtcta actttcttga gcaatcgcgg
     6961 tttattgccc tactgatgta tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt
     7021 attcaaagcg atattcagca ttactggaat ctgcgaattg tcgccagttc gctatgttta
     7081 agccccagcc tgctcaaaaa gaaattaaaa aacgaaaata ccagctatag ccagattgtc
     7141 acagagtgtc gtatgcgtta cgccgtacag atgttattga tggataacaa aaatatcact
     7201 caggtggcgc aattatgtgg ctatagcagc acgtcgtact ttatctctgt ttttaaggcg
     7261 ttttacggcc tgacaccgtt gaattatctc gccaaacagc gacaaaaagt gatgtggtga
     7321 agggcaaagc ggaaacggat aagacgggca taaataaaga agaaatggcg cgccctgcag
     7381 gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg agctaagggc
     7441 gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca
     7501 atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc tgatttattt
     7561 cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt
     7621 catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta tgagactggt
     7681 aactatgaaa ccaacgtcgg tgatcattat ggacactcat cctatcatca gaatgtctat
     7741 tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg atgattaccg
     7801 cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg atatagactt
     7861 gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga gcacagtgaa
     7921 agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga tacaagctgg
     7981 tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg ccgttcagat
     8041 gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa aaagcaataa
     8101 atgtagtacg aatagttcaa cggtcactgt gctatctaat cgtgaagtga ccatattacg
     8161 ttatctagtt agcggattat ctaataaaga aattgccgat aagttattac ttagcaataa
     8221 aacagttagt gcgcataaat ctaatatt
//
LOCUS       DBFIFS010000190         8233 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.190, whole genome shotgun sequence.
ACCESSION   DBFIFS010000190 DBFIFS010000000
VERSION     DBFIFS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.190"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            90..1406
                     /gene="fucP"
                     /locus_tag="N7A07_003778"
     CDS             90..1406
                     /gene="fucP"
                     /locus_tag="N7A07_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU2478361.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1439..3214
                     /gene="fucI"
                     /locus_tag="N7A07_003779"
     CDS             1439..3214
                     /gene="fucI"
                     /locus_tag="N7A07_003779"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU2478362.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3323..4741
                     /gene="fucK"
                     /locus_tag="N7A07_003780"
     CDS             3323..4741
                     /gene="fucK"
                     /locus_tag="N7A07_003780"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU2478363.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4743..5165
                     /gene="fucU"
                     /locus_tag="N7A07_003781"
     CDS             4743..5165
                     /gene="fucU"
                     /locus_tag="N7A07_003781"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU2478364.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5223..5954
                     /gene="fucR"
                     /locus_tag="N7A07_003782"
     CDS             5223..5954
                     /gene="fucR"
                     /locus_tag="N7A07_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU2478365.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(5998..7098)
                     /gene="rlmM"
                     /locus_tag="N7A07_003783"
     CDS             complement(5998..7098)
                     /gene="rlmM"
                     /locus_tag="N7A07_003783"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU2478366.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7091..7486)
                     /locus_tag="N7A07_003784"
     CDS             complement(7091..7486)
                     /locus_tag="N7A07_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU2478367.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7505..>8233)
                     /gene="gcvA"
                     /locus_tag="N7A07_003785"
     CDS             complement(7505..>8233)
                     /gene="gcvA"
                     /locus_tag="N7A07_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU2478368.1"
                     /translation="EEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIH
                     WLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYL
                     LPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHS
                     AMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKI
                     AAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         2052 a         2171 c         2035 g         1975 t
ORIGIN      
        1 tcactgatat gaagcccgaa ctcgctagtt ttgcactttt gaaaacataa ccgattacgt
       61 gcttaagctt ctgaacctaa gaggatgcta tgggaaacac atcaatacaa acgcagagtt
      121 accgtgcggt agataaagat gcagggcaaa gcagaagtta cattattcca ttcgcgctgc
      181 tgtgctcact gttttttctt tgggcggtag ccaataacct taacgacatt ttattacctc
      241 aattccagca ggcttttaca ctgacaaatt tccaggctgg cctgatccaa tcggcctttt
      301 actttggtta tttcattatc ccaattcctg ctgggatatt gatgaaaaaa ctcagttata
      361 aagcaggaat tattaccgga ttatttttat atgccttggg tgctgcatta ttctggcccg
      421 ccgcagaaat aatgaactac accttatttt tagttggcct atttattatt gcagccggat
      481 taggttgtct ggaaactgcc gcaaaccctt ttgttacggt attagggccg gaaagcagtg
      541 gtcacttccg cttaaatctt gcgcaaacat ttaactcatt tggcgcaatt atcgcggttg
      601 tctttgggca aagtcttatt ttgtctaacg tgccacatca atcgcaagac gttctcgata
      661 aaatgtctcc agagcaattg agtgcgtata aacacagcct ggtattatcg gtacagacac
      721 cttatatgat catcgtggct atcgtgttac tggtcgccct gctgatcatg ctgacgaaat
      781 tcccggcatt gcagagtgat aatcacagtg acgccaaaca aggatcgttc tccgcatcgc
      841 tttctcgcct ggcgcgtatt cgccactggc gctgggcagt attagcgcaa ttctgctacg
      901 ttggcgcaca aacggcctgc tggagctatt tgattcgcta cgctgtagaa gaaattccag
      961 gcatgactgc aggctttgcc gctaactatt taaccggaac catggtgtgc ttctttattg
     1021 gtcgtttcac cggtacctgg ctcatcagtc gcttcgcacc acacaaagtc ctggccgcct
     1081 acgcattaat cgctatggca ctgtgcctga tctcagcctt cgctggcagt catgtgggct
     1141 taatagccct gactttatgc agcgccttta tgtcgattca gtacccaaca atcttctcgc
     1201 tgggcattaa gaatctcggc caggacacca aatacggttc gtccttcatc gttatgacca
     1261 tcattggcgg cggtattgtc actccggtca tgggttttgt cagtgacgcg gcgggcaaca
     1321 tccccactgc tgaactgatc cccgcactct gcttcgcggt catctttatc tttgcccgtt
     1381 tccgttctca aacggcaact aactgaacat attttccgaa taaagtgagg aatctgtaat
     1441 gaacaaaatc agcttaccga aaattggtat ccgcccggtt attgacggtc gtcgcatggg
     1501 tgttcgtgag tcgcttgaag aacaaacaat gaatatggcg aaagctacgg ccgcactgct
     1561 gaccgagaaa ctgcgccatg cctgcggagc tgccgtcgag tgtgtcattt ccgatacctg
     1621 tatcgcgggt atggctgaag ccgctgcttg cgaagaaaaa ttcagcagcc agaatgtagg
     1681 cctcaccatt acggtaacgc cttgctggtg ctatggcagt gaaaccatcg acatggatcc
     1741 aacccgcccg aaagccattt ggggctttaa cggcactgaa cgtcccggcg ctgtttacct
     1801 ggcagcggct ctggcagctc acagccagaa aggcatcccg gcattctcca tttacggtca
     1861 tgacgttcag gatgccgatg acacatcgat tcctgccgat gttgaagaaa aactgctgcg
     1921 ctttgcccgc gccggtttgg ccgtcgccag catgaaaggt aaaagctatc tgtcgctggg
     1981 cggcgtttcg atgggtatcg ccggttctat tgttgatcac aacttctttg aatcctggct
     2041 gggaatgaaa gtccaggcgg tggatatgac cgaactgcgt cgccgtatcg atcagaagat
     2101 ttacgacgaa gccgaactgg aaatggcact ggcctgggct gataaaaact tccgctatgg
     2161 cgaagatgaa aataacaaac agtatcaacg caatgccgag caaagccgcg cagttctgcg
     2221 cgaaagttta ctgatggcga tgtgtatccg cgacatgatg caaggcaaca gcaaactggc
     2281 cgatattggt cgcgtggaag aatcacttgg ctacaacgcc atcgctgcgg gcttccaggg
     2341 gcaacgtcac tggaccgatc aatatcccaa tggtgacacc gccgaagcga tcctcaacag
     2401 ttcatttgac tggaatggcg tgcgcgaacc ctttgtcgtg gcgaccgaaa acgacagtct
     2461 taacggcgtg gcaatgctaa tgggtcacca gctcaccggc accgctcagg tatttgccga
     2521 tgtgcgtacc tactggtcac cagaagcaat tgagcgtgta acggggcata aactggatgg
     2581 actggcagaa cacggcatca tccatttgat caactccggt tctgctgcgc tggacggttc
     2641 ctgtaaacaa cgcgacagcg aaggtaaccc gacgatgaag ccgcactggg aaatctctca
     2701 gcaagaggct gacgcttgcc tcgccgctac cgaatggtgc ccggcgatcc acgaatactt
     2761 ccgtggcggc ggttactctt cccgcttcct taccgaaggc ggcgtcccgt tcaccatgac
     2821 tcgtgtcaac atcatcaaag gcctgggacc ggtactgcaa atcgcggaag gctggagcgt
     2881 ggaattgccg aaggatgtgc atgacatcct caacaaacgc accaactcaa cctggccaac
     2941 cacctggttt gcaccgcgcc tcaccggtaa agggccgttt acggatgtgt actcggtaat
     3001 ggcgaactgg ggcgctaacc atggggttct gaccatcggc cacgttggcg cagactttat
     3061 cactctcgcc tccatgctgc gtatcccggt atgtatgcac aacgttgaag agaccaaagt
     3121 gtatcgtcct tctgcctggg ctgcgcacgg catggatatt gaaggccagg attaccgcgc
     3181 ttgccagaac tacggtccgt tgtacaagcg ttaatacatt actccctgat gtgatgcccg
     3241 gtcgctccgg ctgccgggcc tgaacaagca agagtggtta gccggataag caatgttatc
     3301 cggctatatt gcaggagcga tcatgaaaca agaagtgatc ctggtactcg actgtggcgc
     3361 gaccaatgtc agggccatcg cggttaatcg gcagggaaaa attgttgccc gcgcctcaac
     3421 gcctaatgcc agcgatatcg cgatggaaaa caacacctgg caccagtggt ctttagacgc
     3481 cattttgcaa cgctttgctg attgctgtcg gcaaatcaat agtgaactga ctgaatgcca
     3541 catccgcggt atcgccgtca ccacctttgg tgtggatggc gctctggtag ataagcaagg
     3601 caatctgctc tatccgatca ttagctggaa atgtccgcga acagcagcgg ttatggacaa
     3661 tattgaacgg ttaatctccg cacagcggtt gcaggctatt tctggcgtcg gagcctttag
     3721 tttcaatacg ttatataagt tggtgtggtt gaaagaaaat catccacaac tgctggaacg
     3781 cgcgcacgcc tggctcttta tttcgtcgct gattaaccac cgtttaaccg gcgaattcac
     3841 tactgatatc acgatggccg gaaccagcca gatgctggat atccagcaac gcgatttcag
     3901 tccgcaaatt ttacaagcca ccggtattcc acgccgactc ttccctcgtc tggtggaagc
     3961 gggtgaacag attggtacgc tacagaacag cgccgcagca atgctcggct tacccgttgg
     4021 cataccggtg atttccgcag gtcacgatac ccagttcgcc ctttttggcg ctggtgctga
     4081 acaaaatgaa cccgtgctct cttccggtac atgggaaatt ttaatggttc gcagcgccca
     4141 ggttgatact tcgctgttaa gtcagtacgc cggttccacc tgcgaactgg atagccaggc
     4201 agggttgtat aacccaggta tgcaatggct ggcatccggt gtgctggaat gggtgagaaa
     4261 actgttctgg acggctgaaa caccctggca aatgttgatt gaagaggctc gtctgatcgc
     4321 gcctggcgcg gatggcgtga aaatgcagtg tgatttattg tcgtgtcaga acgctggctg
     4381 gcaaggagtg acgcttaata ccacgcgggg gcatttctat cgcgcggcgc tggaagggtt
     4441 aactgcgcaa ttacagcgca atctacagat gctggaaaaa atcgggcact ttaaggcctc
     4501 tgaattattg ttagtcggtg gaggaagtcg caacacattg tggaatcaga ttaaagccaa
     4561 tatgcttgat attccgttaa aagttctcga cgacgccgaa acgaccgtcg caggagctgc
     4621 gctgttcggt tggtatggcg taggggaatt taacagcccg gaagaagccc gtgcacagat
     4681 tcattatcag taccgttatt tctacccgca aactgaacct gaatttatag aggaagtgtg
     4741 aaatgctgaa aacaatttcg ccgttaattt ctcccgaact attgaaagtg ctggcagaga
     4801 tgggacatgg agatgaaatt attttttccg atgctcactt tcccgcccat tcgatgggac
     4861 cgcaggtgat ccgcgctgat ggcctgttgg tgagcgactt gctccaggcg attatcccgt
     4921 tatttgagct ggacagttat gcaccgccgc tggtgatgat ggcagcggta gaaggtgaca
     4981 ctctcgatcc tgaagtagaa cgacgttacc gtaatgcgct ttcactacaa gccccgtgtc
     5041 ctgacatcat ccgcatcaat cgttttgcgt tttatgaacg ggcgcaaaaa gcctttgcga
     5101 tcgttatcac aggcgaacga gcgaagtacg ggaatattct tttaaaaaaa ggggtaacac
     5161 cgtaatctca taccggtacg cccgcatgac gcgggcggtt atcgaatgat ggggtgaaaa
     5221 atatgaaagc ggcacgccag caagcgatag tcgacctgct gctgaaccat accagcctga
     5281 ccacggaagc tctctctgaa cagctaaagg tcagtaaaga aaccattcgt cgcgatctca
     5341 atgaattaca gacgcagggt aaaattctgc gcaatcatgg acgcgctaaa tatatccacc
     5401 gtcaaaatca agacagtggc gatccctttc acatcaggct gaaaagccat tatgcgcata
     5461 aagcagatat cgcgcgcgag gcgctcgcgt ggattgaaga agggatggtg atagccttag
     5521 acgccagttc aacttgctgg tatctggcac gccagttgcc tgacatcaac attcaggtct
     5581 tcaccaatag ccatccgatt tgccatgaac tcggtaaacg cgaacgcatt caactgatca
     5641 gttccggcgg cacacttgag cgcaaatatg gctgttacgt caatccctcg ctgatttccc
     5701 aacttaaatc gctggaaatc gatctgttta ttttttcttg tgaagggatc gatagcagcg
     5761 gcgcactgtg ggactccaat gctatcaacg ctgattacaa atcaatgcta ttaaaacgcg
     5821 ccgcgcaatc gttgttattg attgataaaa gtaaatttaa tcgttcaggg gaagcccgca
     5881 tcgggcatct ggatgaggta acgcacatta tttctgatga acgccaggtt gcaacttctt
     5941 tggtaacagg ctgacggaaa gggtagcagg ccggagacga cgccccggcc ttgcctgtta
     6001 tcgctcgtcg cgacgaccac ccaccgcagc ccagatacgg cggacgtgca ccgtcacttc
     6061 ttcgcgatcg tgatacaact gccgcgcctg aatctgagca tttatgccat gttcatcaag
     6121 ctgtgcctga atatacgcca gattgtgtga cacttcttcg tagcgttttt tcatcggcag
     6181 tttgaggttg aaaatggttt cacgacacca gccattaacc agccactgcg ccatcaatgc
     6241 cgcaactttc gccggttttt caaccatatc gcataccatc caggagatat tgctgcgcgt
     6301 cggacggaat ttgaaaccgt cttcccgcag ccacgtcacc tgtccggtat ccatcagact
     6361 ttgcgccatc gggccgttgt cgacggaata aacccacatg ttgcgcttca ccagttggta
     6421 ggtccagccg ccagggcaag cgcctaaatc caccgcccac atcccgttcg ccaggcgttc
     6481 atcccactca tccgcaggaa taaacacatg aaatgcctct tccagtttga gcgtggaacg
     6541 actcggcgca tctgccggaa atttcaggcg cggaatgccc atatagaacg gcgaattatt
     6601 gttgctgtat gagtaaccgg tatagcagca gcctggtgca ataaagaata catgcacaac
     6661 cggacgcttc ggcgtttcat agttcgccag caccccggca tcgcgcaagg cagcgcgtag
     6721 cggcacggtg aatttacggc agaatttcag caactctttg ctttcgttgg tatcggcaac
     6781 ttcaacacgc agttcaccgc ccttctctac tacgccctgc aacatgccga caatgggggt
     6841 aatacgatct tctggcggca aatgctgcaa gagttccccc accacaaacc actggcgggc
     6901 aaaaattaat gaactgaacg gcagctcacg gattaactta tcgccatcat caggttgata
     6961 acattcataa atgacataac ccgcattctc tttcacgcgg gcaaaaccga aaatctcccg
     7021 ctggccggct ttatcggtaa tttctgcggc gcactctttt tcaaagcccg gacggcacag
     7081 caatacaacc ttattcatga cttacgccct tgcgctttaa acggatagca ccaactaaca
     7141 ttaacgccca gcccgcaagg aagctcacgc cgccaaccgg agtgacaaac gcccacaaac
     7201 gcagatggga cagtgccagg caataaaggc tgccgctgaa cagcaccgtg cctaacgcga
     7261 ggaaaacgct actccagtaa aaccagatac tgatgcgacg ctgcattgcc accgccagac
     7321 ctaagatcgc cagcgtatga aacgcctggt attcgaggcc ggtctggatc cagcccatct
     7381 caacggcccc catggtttta cttaacacat gcgcgccaaa agcgcccagt gccacaaaaa
     7441 tgaagccgct aatggcggcg aaaatcagca taaaacggct ggtcatggtc gtaccctacg
     7501 taaattattg ttcataacga aagcggaatt tttcttgttc agcagcggct ttcgccagga
     7561 tccattggcg aaaggcggct attttaccca gttctgcctg gctgtcatga caaaccagat
     7621 aaaaagcgtt tttactgacc agaacatcat taaacgggca aacaagacgt ccggcttcaa
     7681 tttcagattg cgccatcacg ttatttgcca gcgccactcc ctgcccgtga atagccgctt
     7741 gcagcaccat ggcgctatgg ctaaaaattg gcccttgctg aacgttgata tgatttaacc
     7801 ccaattgacg agtatatgtc tgccagtcac ggcgtgacgc atcatgtaat aacgtatgtt
     7861 tagccagatc ttccggggtc ttcaagggtt tttcgccagt cagcagtagc ggcgaacaca
     7921 ccggcaataa atattcggcg tacagttttt ccacccgtag ccccggccag ttgccccgac
     7981 cataaaatat cgccacatca acatcatccg ccagcttatc ttcctgacga tcaaccgcct
     8041 ggattcgaac gtcaattccc ggataagctg aattaaagct ggaaagtcgc ggaaccaacc
     8101 aatgaatggc gaaactgggg agtaaactga ccgtcaacgc ccccttggcg ctacgggcct
     8161 ggagtttacg cgtcgcttcg gttaattgcg aaaatatctc tttgatatcg aggaaatagc
     8221 tttgcccttc ctc
//
LOCUS       DBFIFS010000191         8149 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.191, whole genome shotgun sequence.
ACCESSION   DBFIFS010000191 DBFIFS010000000
VERSION     DBFIFS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.191"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..305
                     /locus_tag="N7A07_003786"
     CDS             <1..305
                     /locus_tag="N7A07_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478369.1"
                     /translation="DNLRTFNTNELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPT
                     KDTNWNSTYIYKSRHEMLPVNLTPETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            528..1025
                     /gene="ubiC"
                     /locus_tag="N7A07_003787"
     CDS             528..1025
                     /gene="ubiC"
                     /locus_tag="N7A07_003787"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU2478370.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1038..1910
                     /gene="ubiA"
                     /locus_tag="N7A07_003788"
     CDS             1038..1910
                     /gene="ubiA"
                     /locus_tag="N7A07_003788"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU2478371.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2065..4488)
                     /gene="plsB"
                     /locus_tag="N7A07_003789"
     CDS             complement(2065..4488)
                     /gene="plsB"
                     /locus_tag="N7A07_003789"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU2478372.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4659..5027
                     /gene="dgkA"
                     /locus_tag="N7A07_003790"
     CDS             4659..5027
                     /gene="dgkA"
                     /locus_tag="N7A07_003790"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU2478373.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5137..5745
                     /gene="lexA"
                     /locus_tag="N7A07_003791"
     CDS             5137..5745
                     /gene="lexA"
                     /locus_tag="N7A07_003791"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU2478374.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5818..7143
                     /gene="dinF"
                     /locus_tag="N7A07_003792"
     CDS             5818..7143
                     /gene="dinF"
                     /locus_tag="N7A07_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU2478375.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7259..7468
                     /gene="yjbJ"
                     /locus_tag="N7A07_003793"
     CDS             7259..7468
                     /gene="yjbJ"
                     /locus_tag="N7A07_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU2478376.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7510..8025)
                     /gene="zur"
                     /locus_tag="N7A07_003794"
     CDS             complement(7510..8025)
                     /gene="zur"
                     /locus_tag="N7A07_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU2478377.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1839 a         1995 c         2236 g         2079 t
ORIGIN      
        1 aagataattt aagaaccttt aacacgaatg aattaacacg aaacgataat atctttatct
       61 tgttctcctc tgtcgatgat gggccagtta tggtggtaag ctcccagcgc ttacatgata
      121 tgttgaatcc tacaaaagat accaattgga attccacgta tatctataaa tccagacatg
      181 agatgttgcc tgttaatctt actccggaaa cacttttcgg ctccaaatct tatgataaac
      241 atgcgctttt ccccattttt actgcgagtt ggcgagctaa tcgtataaag aataaaggta
      301 tttaagtaaa ggaaaacatc accgctcttg gcatcatggg cggtgatgcc cctatggttg
      361 ccctcgccag cacgggcatc ggcaaagcgt aaggttcaac agcgttttac cacttcatgc
      421 gattgttgcg tttttgttgc gcattagatc acttaatttg ctttacatct cccgtaaaca
      481 cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg tcacaccccg
      541 cgttaacgca actgcgtgcg ctgcgctatt gtaaagagat ccctgccctg gagccgcaac
      601 tgctcgactg gctgttgctg gaagattcca tgacaaaacg ttttgaacag cagggaaaaa
      661 cggtgagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc cccgaagaac
      721 tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta tgtgccgatg
      781 gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc gggccggagc
      841 tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca tcatcgacat
      901 taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga cgttcccgct
      961 tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg tcaccgttgt
     1021 actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg gcgtttcatc
     1081 gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca acattatggg
     1141 cgttgtgggt ggcgacaccg ggcgttcccc agctctggat cctggcggtg tttgtcgcgg
     1201 gtgtctggct gatgcgcgct gccggatgtg tggtgaatga ttatgctgac cgcaagtttg
     1261 atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta acagagaaag
     1321 aggcgcgcgc gctgtttgta gtgctggtac tgatttcgtt tttactggtg ctgacgctga
     1381 atacgatgac cattctgttg tcgattgccg cgctagcgct ggcgtgggtg tacccgttta
     1441 tgaagcggta tacccatcta ccgcaagtgg tgctgggcgc ggcgtttggc tggtcgattc
     1501 caatggcttt tgctgctgtg agtgagtcgg tgccattgag ttgctggtta atgttcctcg
     1561 ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac cgtgatgatg
     1621 atgtgaagat tggcattaaa tccaccgcaa tcctgttcgg ccaatacgat aaattgatta
     1681 ttggtatttt gcagattggc gtactggcgc tgatggcgat tatcggtgag ttaaatggct
     1741 taggctgggg atattactgg tcaattctgg tggctggcgc gctgtttgtt tatcaacaaa
     1801 aactgattgc caaccgcgag cgcgaagcct gctttaaagc atttatgaat aataactatg
     1861 ttggtctggt actgttttta gggctggcaa tgagttactg gcatttctga tgatgtaaaa
     1921 aagccggatg atcatccggc tttcttctgg gttgcctgat gcgctgcgct tctcaggcct
     1981 acacaacaga tcgcaattta ttgaatttgc agattatgga aggccggata aggcgtttgc
     2041 gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct caatcgtcaa
     2101 acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg tttctgccgg
     2161 ttcggcatcg ccgctatcgc tgatataccc ttcatcacgc agcgtaagca ccagagaact
     2221 gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg agagacgttg
     2281 cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt tggcgctcaa
     2341 cagccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg cgagcagctg
     2401 tagcgtgcga gaatgcaccg ggttgatatg caactcatca tcttgcaggg taatcagccc
     2461 ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt cgcgatccca
     2521 gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct ccatcaatac
     2581 gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag gtagcaccaa
     2641 catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc gcggcagaat
     2701 gatgatgtcg ccgatagtgt ctttctcaac ttcaaacttg ttcatttgca gcgcgtgatc
     2761 gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag gcacgttgcg
     2821 catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga gtgagcgctg
     2881 acgtgatgcc agtagcgcag tacagcacag gttcatggcg tttgccgcgc ctgcgttgtt
     2941 aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc atgccggacg
     3001 cactgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt taaggtaggt
     3061 catcaacggc attggttcac cgaaattgac gtaaccctgg ccgagattac gcagcttgct
     3121 taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcgccgc gcagttcttt
     3181 ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa tcagcgtaat
     3241 cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac cggttttcgg
     3301 atccagcaaa cgccccgtac gggaacgacc gccttctacg aagtactcga cggaataacc
     3361 acggctgaac agttcgccga gatactcacg gaaaacggtg gaatagagct tattgccttt
     3421 aaaagtacgg cgaataaaga acgcacccag acggcggaaa atcggcccgg caggccagaa
     3481 attcaggttg atcccggcgg cgatatgcgg cggcactagc ccctggtggt acagcacgta
     3541 agaaagcagc aggtagtcca tgtgactgcg gtggcaaggc acatatacca gctcatgacc
     3601 gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct ggtaaaggcg
     3661 gttccaggta aagcccagaa tacggtcagt caggcggatc atctcgtaag agaaattcgc
     3721 cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg agattttttt
     3781 gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca gcttattaaa
     3841 cagatcctga cgagcaggca gacgtggtcc tacggcagcc agacgttgac gggcaaagtg
     3901 catacgcgcc acgcgcgcca gtttctgcgc gatagtttta tccgtgccgt gttcatccgc
     3961 catacggcgc agcgaaactg acggcgagaa acgcacaaaa ctgtcgcgac cgagccacag
     4021 tacagcgaaa aatttctgta cgccgttaag catacgcagc ggcgggttca cttcgccttt
     4081 ttcacgcccc ggcgcgcgac caaacatcac cgacactggc accatctgca catccagatt
     4141 tgggttgcta cggtgcaaat cgagatagtc gtggaacagc ttaatagact cttctttcgg
     4201 cgtgtaatag gtgaatacac gcggcccgcc gtgaatgaac acatagcgcg gcagtagcgt
     4261 gccgtcgatt tccagcggct ctaacgggtc aggcaagtca tgtgccagac actgggcgcg
     4321 caacgtcagc aaatctgctt tcgagttgta cggtaaaacg tacataattg gacgagaggt
     4381 atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta ccaggatgct
     4441 taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa acgatgtaaa
     4501 gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg gaaattctgt
     4561 ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat ttaacggtac
     4621 actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat accactggat
     4681 tcacccgaat aatcaaagct gctggctatt cctggaaagg tttacgcgct gcatggatca
     4741 acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc atcgcctgct
     4801 ggctggatgt ggacgcgatt acccgcgtgc tgcttatcag ctccgtgatg ctggtgatga
     4861 ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc tctgaatacc
     4921 atgagctttc cggacgcgct aaagatatgg gatccgccgc ggtgctgatt gcgattatcg
     4981 tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc ttccagaatt
     5041 cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt gctgtatata
     5101 ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac ggccaggcaa
     5161 caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt
     5221 gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag
     5281 gcgctggcac gcaaaggcgt aattgaaatt gtttccggcg catcacgcgg gattcgtctg
     5341 ttgcaggaag aggaagaagg gttgccactg gtaggtcgtg tggctgccgg tgaaccgctt
     5401 ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt caagccgaat
     5461 gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat tatggatggc
     5521 gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt
     5581 attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg
     5641 ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag cttcaccatt
     5701 gaagggctgg cggttggcgt tattcgcaac ggcgactggc tgtaacatat ctctgagacc
     5761 gcgatgccgc ttggcgtcgc ggtttgtttt tcatctctct tcatcaggct tgtctgcatg
     5821 gcattcctca cttcatctga taaagcactt tggcatctcg ccttacccat gattttctcc
     5881 aatatcactg ttccgttgct gggactggtc gatacggcgg ttattggtca tcttgatagt
     5941 ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct ctttatgctg
     6001 ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg tgccaaaaat
     6061 cctcaggcat tagcccgtgc gctggtgcaa cctttgctgt tggcgttggg ggctggggcg
     6121 ttaattgcgc tgctgcgtac gccgattatc gatctggcgc tgcatattgt tggcggtagt
     6181 gaagcagtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag cgcaccggcg
     6241 tcgctggcga atctggtatt acttggttgg ttactcggcg tgcaatatgc ccgtgcgcca
     6301 gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg gctggtgatg
     6361 ggactgcata tgaacgtgcg gggcgcggcg ctggcgacgg ttattgcgga atatgcaaca
     6421 ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat ctccggcgaa
     6481 atgctgaaaa cttcctggcg aggaaacttc cgtcgcttgc tggcacttaa ccgcgatatc
     6541 atgctgcgct cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact tggcgcgcga
     6601 ctggggagtg acattatcgc tgttaacgcg gttctgatga cgctactcac ctttaccgcc
     6661 tatgcgctgg acggttttgc ctacgcggtt gaagcacatt ccggtcaggc atacggtgcg
     6721 cgcgacggta gccagttgct ggatgtctgg cgggcagcat gccgccagtc ggggatcgta
     6781 gcgttactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc gttactggcg
     6841 tcgttaaccc agattcagca gctggccgac cgctatctta tctggcaggt gattttgccg
     6901 ttggtcggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac gcgtgctgct
     6961 gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct ccttacgctg
     7021 ccgtggctgg gtaatcatgg tttgtggttg gcgttaaccg tctttctggc gttgcgcggg
     7081 ctttctctgg cagctatctg gcggcgtcac tggcgcaacg gtacctggtt tgccgcaacg
     7141 tgacggttaa aaattctgaa taaataatcc taagccaaat tgctgactac acttaatctc
     7201 acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg tcttgattat
     7261 gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga aagagcaatg
     7321 gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc aactggtcgg
     7381 taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg tcgtggattg
     7441 ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg tacaaggatg
     7501 tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct ggcactgttc
     7561 aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg cttcaatcac
     7621 attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcatgatgt cttccacgcc
     7681 ttcagcacat tcttctttca ccgcgccgca gcgatcgcaa ataaacatag ctgacgtatg
     7741 ggtgggctgg tcgaacagat ggcagagcac ataactgttg gtggattcca ccttatgcac
     7801 aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct tggcttgcgg
     7861 ttcagcttcg cgcagcaaat caagcagatc ataagcgctg atagcgccat cctgcagact
     7921 catcaggcgc aacacttcca ggcgctgtgg ggtcaggcgc acattacgct gcgcgcagat
     7981 tttgtcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc acctcaaagt
     8041 gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga acccttattg
     8101 ttgccgtaat gttgcttttc tgttttgtag gtaagatgtt gtgttgcct
//
LOCUS       DBFIFS010000192         8133 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.192, whole genome shotgun sequence.
ACCESSION   DBFIFS010000192 DBFIFS010000000
VERSION     DBFIFS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.192"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(58..249)
                     /locus_tag="N7A07_003795"
     CDS             complement(58..249)
                     /locus_tag="N7A07_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2478378.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(246..434)
                     /gene="dicB"
                     /locus_tag="N7A07_003796"
     CDS             complement(246..434)
                     /gene="dicB"
                     /locus_tag="N7A07_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2478379.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1008..1193
                     /locus_tag="N7A07_003797"
     CDS             1008..1193
                     /locus_tag="N7A07_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478380.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1380..1769)
                     /locus_tag="N7A07_003798"
     CDS             complement(1380..1769)
                     /locus_tag="N7A07_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478381.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1781..1909)
                     /gene="ydfB"
                     /locus_tag="N7A07_003799"
     CDS             complement(1781..1909)
                     /gene="ydfB"
                     /locus_tag="N7A07_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU2478382.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1911..2066)
                     /locus_tag="N7A07_003800"
     CDS             complement(1911..2066)
                     /locus_tag="N7A07_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2478383.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2344..2631)
                     /locus_tag="N7A07_003801"
     CDS             complement(2344..2631)
                     /locus_tag="N7A07_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2478384.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2631..2822)
                     /locus_tag="N7A07_003802"
     CDS             complement(2631..2822)
                     /locus_tag="N7A07_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU2478385.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2850..3251)
                     /locus_tag="N7A07_003803"
     CDS             complement(2850..3251)
                     /locus_tag="N7A07_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478386.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3360..3632
                     /locus_tag="N7A07_003804"
     CDS             3360..3632
                     /locus_tag="N7A07_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2478387.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3616..4041
                     /locus_tag="N7A07_003805"
     CDS             3616..4041
                     /locus_tag="N7A07_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2478388.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4248..4703)
                     /locus_tag="N7A07_003806"
     CDS             complement(4248..4703)
                     /locus_tag="N7A07_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478389.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4782..5873
                     /locus_tag="N7A07_003807"
     CDS             4782..5873
                     /locus_tag="N7A07_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2478390.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5880..6626
                     /locus_tag="N7A07_003808"
     CDS             5880..6626
                     /locus_tag="N7A07_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2478391.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGLKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6648..7418
                     /locus_tag="N7A07_003809"
     CDS             6648..7418
                     /locus_tag="N7A07_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2478392.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7434..7847
                     /locus_tag="N7A07_003810"
     CDS             7434..7847
                     /locus_tag="N7A07_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2478393.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2183 a         1733 c         2109 g         2108 t
ORIGIN      
        1 aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg tgctgtttta
       61 aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg gataacagtt
      121 accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac cgtataagcc
      181 agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga gtgcaaatgt
      241 tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat tgaaggaatg
      301 catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc ttcaataaat
      361 gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct ggcattacat
      421 gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg cctggtgcct
      481 ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc gttactcagc
      541 aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc accgcatcac
      601 aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga taccgccaaa
      661 tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta agcgtatggt
      721 caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac ttaccgccgc
      781 gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta acgacggctc
      841 taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc agggatatat
      901 cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga ccacacgtag
      961 cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg ccaaacaaac
     1021 catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat tacattttta
     1081 actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt acaaagccaa
     1141 atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat taagggcgta
     1201 gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa accagtaatt
     1261 ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa gttcgggagg
     1321 aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg cagtcccttt
     1381 catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg tgtcatctga
     1441 ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc gctctgcaat
     1501 ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg aaaacccatc
     1561 accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac cgaaacagag
     1621 tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta tttcttcgtt
     1681 gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc aatttcctgg
     1741 atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta gattttgccg
     1801 aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct tcggcaaggc
     1861 cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata tcactcacct
     1921 gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt gcttttggta
     1981 tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac cagagattcg
     2041 ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc cgggtagcgg
     2101 aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc atacgcctcg
     2161 ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc ggcgttctgt
     2221 ggggcggggt gggtggttgg tgtatgtaat ctacaataaa aaactgtttt tgtgtcaaca
     2281 gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg aaaattgttc
     2341 aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa tctcaatgct
     2401 tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct ctctcaatcc
     2461 agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta ataccgatgt
     2521 ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc tccttggcga tgtaagttat
     2581 gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca ttactcgctc
     2641 ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac ctcgtcatgt
     2701 ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa ccattcgttg
     2761 taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag ggctgtaccc
     2821 atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg tgccgccagc
     2881 cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg aagcattttt
     2941 tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg gcgaggacca
     3001 attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa taaaaaccaa
     3061 tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt aggtgtagcc
     3121 ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg ggcaagctca
     3181 gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga tacttctagg
     3241 gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca ataaaaaact
     3301 gttgattgca tacagttttt tattgtaggc tttgcttata gtttttagag gagggcaaaa
     3361 tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa ataggtcgtt
     3421 atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt cccccaaagc
     3481 aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa atccgcccag
     3541 atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac acatcaaatg
     3601 cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat gaatgcctgg
     3661 gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta tttcgagctg
     3721 ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc tcgcaatact
     3781 cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa aattcaggcg
     3841 ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat gcgcagccac
     3901 agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa agatattgat
     3961 gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg cccggcagga
     4021 aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa aattgacact
     4081 gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg tggagctggc
     4141 agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca gagaacagcg
     4201 caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta ttgaggatta
     4261 tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga gcagggagtc
     4321 aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg aggtgggtat
     4381 tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa gaaaatcagt
     4441 tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg cgataccaga
     4501 atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt ttgaagttcg
     4561 ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct gtaatccggg
     4621 agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac atgagcctga
     4681 taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt gggaaccaca
     4741 gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat tacgcaaaag
     4801 tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg ggtgcagaag
     4861 cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg ggtttgtatt
     4921 acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg gcttcaaagg
     4981 ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca gagatggtct
     5041 gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc gataaccgtt
     5101 gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg tcagtgtttt
     5161 acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc cgaaattcgg
     5221 taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa caggagcagg
     5281 agaaagaaca ggaacaggac aaaaacacta tggttcatgg caaaaaaaac accacgaacc
     5341 aggcagggga tgttcagacc gtcaatcctg gtcagccagc aggcacgaca ccggaagccg
     5401 attcgggcgc tgtgcagcag gtgatgaccg cagggtcgga gcaatcacac caactgcagc
     5461 agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg gaaagcaccg
     5521 ggcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg cgggaatacc
     5581 cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag gccagattgc
     5641 gcgagggggt gccaccagag accatgctgg atggtgtgag gcgttacgcg agatacctgg
     5701 cggcgaccgg gaaagcggga acggaatttg ttcagcgagc gacgacgttt tttgggccgg
     5761 accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac cagcgttgtg
     5821 tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg tgatgtggca
     5881 tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca ccacaggcgt
     5941 cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgct gaaggacgaa
     6001 aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa atcctgaatc
     6061 gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag gtgcagaacg
     6121 acgggctaaa atacgcgctg agccaggcca aatccatagc tgacgaactg atgaccgggt
     6181 gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt gcagcggcga
     6241 tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg tctgatgtca
     6301 tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt ttacaggagc
     6361 tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag acgaaaaacg
     6421 agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc agtgtcggga
     6481 tgttaacaaa cctgaatcat gccgcaatga gtacacttct tggtgagagg attatggacc
     6541 gcatgaccat gaacggtggt cgatgggtga cgtttaactg ggatagctgg cgtccaaatg
     6601 tcagcaatat gagggttgtg aagtaatttt gtccggagga aattttaatg gaaaccgtat
     6661 ctgacgcact gaaagcactg aaaaaagcct cttcacatgt ggtggcagct cgccttggaa
     6721 tcagtcgtga agaggctgtc aacgagctgt gggaactcaa aataaatggc gtcgttgata
     6781 aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc gaagagcggc
     6841 cagtaaaatc tgaagcacag gatatgctga ccggggaggt cgaacaaaaa gttaccgcag
     6901 acatgatgat tgagtttatc ggtcaggatg gggctaaaac gtgtgaggaa ctggcgggta
     6961 agttcggtgt cagtactcgc aaggttgctt ccacgctggc ggtggtaacc gcaacggggc
     7021 ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc gataatttac
     7081 cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg taaggccttt cctcagccag
     7141 caggtgctgc gttaccagtc cgggaagccg caacacagga agaaattaaa acagaaactg
     7201 tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac gcaagcagat gagctgattt
     7261 ttccgtccct tcgcagggca aacctggcgc tgcgcagggc gaaaagtgat gttcagaagt
     7321 gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatt gttcgacaga
     7381 ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg cttatggcaa
     7441 aagtatttac acaggaagag cgggaaaaaa ttaaagggca ggttgttgag ctagtacgcc
     7501 ggagtgggcg cgagacgtta cggcaactgg aagccaagac aggtgcgaca agatatctga
     7561 tgagcgttct tgccagagag ctggttgcca gtggcgatgt atacaactct ggttacgggt
     7621 tattcccatc tgaacaggct cgtaaggact ggcaaaacgc ccgcaaaaaa ctatcgaggg
     7681 caaaactgaa gaaaccggtt gtggttgatc ctgaccttat ctggtcatta ccagacggag
     7741 aaatacgccg ctatgacagg caattgaaca taatttgtag tgagtgccgt aacagtgaag
     7801 tgatgcagcg agttttgata ttttacacag gagtaatgat ggaatagtga aaataacatg
     7861 aaacctttgg attctggtgt ttcagtggat gggaaaagag cagatacata gaaaatgaat
     7921 agcaataatt cacagtctgg atgttgtttg tatgcttata aaatgatcct agtatgcatt
     7981 gccggagttg aatcagctcc aatgaagaaa ctgagaggag tatttagaag atagctaaat
     8041 ggatgtaatt attagtatct aaaaaatagt atttttgaaa tgggtctaag aacacaacaa
     8101 tgtacaaatg agaagttatc ttttaatatg ttc
//
LOCUS       DBFIFS010000193         8106 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.193, whole genome shotgun sequence.
ACCESSION   DBFIFS010000193 DBFIFS010000000
VERSION     DBFIFS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.193"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..152)
                     /locus_tag="N7A07_003811"
     CDS             complement(<1..152)
                     /locus_tag="N7A07_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methylase"
                     /protein_id="HGU2478394.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELNRPG"
     gene            complement(379..546)
                     /locus_tag="N7A07_003812"
     CDS             complement(379..546)
                     /locus_tag="N7A07_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478395.1"
                     /translation="MKFKFTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQAA"
     gene            complement(589..1212)
                     /locus_tag="N7A07_003813"
     CDS             complement(589..1212)
                     /locus_tag="N7A07_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2478396.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFVESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMEKAQDISKRLYPILKELNSSYASKLYD
                     IAFETFYTVTKNRDALLREATRIDQTSAIFERARPMLKSLRARQFEF"
     gene            complement(1568..1852)
                     /locus_tag="N7A07_003814"
     CDS             complement(1568..1852)
                     /locus_tag="N7A07_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU2478397.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYLAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGSIIVVTTQSDMTIQIHKPKEEGRG"
     gene            complement(1852..2325)
                     /locus_tag="N7A07_003815"
     CDS             complement(1852..2325)
                     /locus_tag="N7A07_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2478398.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVN"
     gene            complement(2338..2469)
                     /locus_tag="N7A07_003816"
                     /pseudo
     CDS             complement(2338..2469)
                     /locus_tag="N7A07_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2473..2760)
                     /locus_tag="N7A07_003817"
     CDS             complement(2473..2760)
                     /locus_tag="N7A07_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478399.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            complement(2762..2980)
                     /locus_tag="N7A07_003818"
     CDS             complement(2762..2980)
                     /locus_tag="N7A07_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU2478400.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMTPISPLIFVWAIGKII
                     ELVIELYNDVVWASFNTLHNKINPYKEN"
     gene            complement(2982..3197)
                     /locus_tag="N7A07_003819"
     CDS             complement(2982..3197)
                     /locus_tag="N7A07_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478401.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQTPDFGEYIGTSWAGCAKEAR
                     KDGWRINKDKTRAFAPGHKILRSNKGE"
     gene            complement(3217..3663)
                     /locus_tag="N7A07_003820"
                     /pseudo
     CDS             complement(3217..3663)
                     /locus_tag="N7A07_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3665..4612)
                     /locus_tag="N7A07_003821"
     CDS             complement(3665..4612)
                     /locus_tag="N7A07_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2478402.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVISDGSKHIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDYD
                     RIVWVDEDGNEANKLQSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            complement(4609..4830)
                     /locus_tag="N7A07_003822"
     CDS             complement(4609..4830)
                     /locus_tag="N7A07_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2478403.1"
                     /translation="MADLIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(4929..5144)
                     /locus_tag="N7A07_003823"
     CDS             complement(4929..5144)
                     /locus_tag="N7A07_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2478404.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(5221..5412)
                     /locus_tag="N7A07_003824"
     CDS             complement(5221..5412)
                     /locus_tag="N7A07_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2478405.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(5385..5567)
                     /locus_tag="N7A07_003825"
     CDS             complement(5385..5567)
                     /locus_tag="N7A07_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2478406.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            complement(5564..6244)
                     /locus_tag="N7A07_003826"
     CDS             complement(5564..6244)
                     /locus_tag="N7A07_003826"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2478407.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARVLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYLAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(6241..7026)
                     /gene="bet"
                     /locus_tag="N7A07_003827"
     CDS             complement(6241..7026)
                     /gene="bet"
                     /locus_tag="N7A07_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2478408.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            complement(7032..7328)
                     /locus_tag="N7A07_003828"
     CDS             complement(7032..7328)
                     /locus_tag="N7A07_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU2478409.1"
                     /translation="MNAYYIQDRLEAQSWERHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(7383..7547)
                     /gene="kil"
                     /locus_tag="N7A07_003829"
     CDS             complement(7383..7547)
                     /gene="kil"
                     /locus_tag="N7A07_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU2478410.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            complement(7516..7680)
                     /locus_tag="N7A07_003830"
     CDS             complement(7516..7680)
                     /locus_tag="N7A07_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU2478411.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            complement(7753..>8106)
                     /locus_tag="N7A07_003831"
     CDS             complement(7753..>8106)
                     /locus_tag="N7A07_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU2478412.1"
                     /translation="KYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYRLNKHG
                     SLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIRITDID
                     TSGIFDSDDMTIKAA"
BASE COUNT         1900 a         2019 c         1801 g         2386 t
ORIGIN      
        1 ccggggcggt tcagttccca taccgggagg cggcagatat ccagaagact catgaccgga
       61 tatttaaaac ttgcaccgcg ttgaccatcg gttgctttgt ctcggaatgt ccagggggga
      121 tctgcgtaaa tgagagtgta ttttttcgtc atagcgcgtt ttcaaactca tcaatgtaaa
      181 gtgcaacaag tgcaacgcat cgtctgaaat cggctgcgtc atgtggagca ccgaagctat
      241 gaccatagat attctttttc agtccacatg cgattgaggc aagagttgcg ctactgatgc
      301 caacatagtc agtcgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
      361 tccaataaaa aaccgccatc aggcggcttg gtgttctttc agttcttcaa ttcgaatatt
      421 ggttacttct gcatgtgcta tctgcgccca tatcatccag tggtcatagc agtcattgat
      481 attctccgct tcgataactc tgttgaatgg ttctccattc cattcacctg tgaatttgaa
      541 tttcattaag tttcacctgt aaaaaaagga gccgaagctc ctttgatatt aaaattcgaa
      601 ttgtctcgcc cgaaggcttt ttaacattgg tcttgcccgt tcgaaaatgg cgcttgtctg
      661 gtcaattcgt gttgcctccc tgagcaatgc gtctctgttt ttcgtcaccg tgtagaaagt
      721 ctcaaacgcg atgtcataca gcttgctcgc gtatgatgag ttcagttcct tcaatatcgg
      781 atacaggcgt ttgctgatgt cctgtgcttt ctccatctgt acctgcatgt agcagaggag
      841 gatgatttcc tcgtctgtga attgctgtgc tggttgcatg ctgcgaagtt tcttttcgca
      901 ctcgatgaag tatcggcgta tctggcggcc tttttcgtta cgctcaacca tcgccagttc
      961 tttggctgtg tcgagagtta ggtggtagtc cttgctacgg cgatcacctc ctcgcccttt
     1021 ggtttgccca ttttggcaaa tcaaaatata gtcctggctt tcaacgaatc catattcagc
     1081 aatgcgttct ttaacccagt gcgcgaacat tctgcccaca ccaagaaaag agtgtagatc
     1141 acgagcgtta actagaagaa ttgtttcgtt gtcgatagtg ccgttgaata cggggatgag
     1201 ttgactggtc atgatgacct cctttgaagt tttagttagc aatcaccagt tagtggctgg
     1261 tgatcgggtg tcaactagag ccttcaaaga tgctccgggc atattcccct tgcgggtatt
     1321 gtattacgcc tctccacccg acctttgtac ggatgtgact atgccaaatt gcaggcataa
     1381 aaaagccgca aagctatcgg gtgcggatga ccgctttgaa gttctagtgc ggtcagtatg
     1441 cgatagctct ggcggatttg tcaaatcatg taggccttat cttgctgtaa gccgcgccat
     1501 tcgggctttt ccccagattt gggggaaact atctgcgaag tattcacctt taacggcaag
     1561 ttgcaggtta gccacgacct tcctcctttg gcttgtgaat ttgtatcgtc atgtcgcttt
     1621 gagtggtgac tacaatgata gaaccaggct gaaggctgtt aagattgaat gcttcgtaaa
     1681 acgaatccaa ggccagcgct tttttattct ttcggttcca ccaacgccat cccttgctac
     1741 aggctacact gacaatccac tgtccactcc tataagccaa ataaaaccag atgagcaaaa
     1801 cctgaaggaa tgctatccag tcaataatcg tatatttcgc gaaggagtcc atcaattaac
     1861 ctcctgcggc ggttctggta gcggcatcca gtgagttgct tgctcaatac cattacccgg
     1921 cttaatcgtt gcatctccgc gccgaaaggt gcttccggta tagcgtgcgg agcatattag
     1981 cggttcaacc agagagctat cgaaattcac cgaaataagc acgttctggc ccttttcagg
     2041 cattcgatca ctacagctta tccaactatc cggagttacc ggagagttac cagcctcata
     2101 agcggatttc atccagtgcg taagcgtttc gatgcttaca catccgcaat caacgtctat
     2161 tttttctttt tgttctgaca accattcctg gaatgacagc ttggcagtct ggcttgctgg
     2221 atcaattcgt ggcaggccga tatatagtgg tacatttccc ggctccatcg aattgtcggg
     2281 acaaataaac gtgttacaac catatttaac gagctcaatt cccactgtgt cgatagtggc
     2341 gaatggttca gtggtcaatg cagtcaacgc aatttcataa gcacggcgct caatattatc
     2401 tcgcacgtcc aggctgccga ttcgctcttt gatttcttta atcatttctt tgtcggtgaa
     2461 cgttgtcatg tgttagtcct tatccacttc aacgccatct ttcagcgtga tgccgtgcca
     2521 atcatcagcc caactggtta acccaggcgc atcaatgcta ggcatataga cgcttgcagt
     2581 gtggtagccc ttatcgttat caatgctggc aacgtgctcg ccgttgtatg cgctcagcgt
     2641 gtctaggaca ctataaaact ttcctccggc tgccctgaaa tcctttacag ccttcacaag
     2701 acgattccac gctttttcct gttctggcgt caggtcgatt aattcctgca aagttgccat
     2761 ttcagttttc cttatatggg ttaattttat tgtgcagtgt gttgaacgac gcccatacca
     2821 cgtcgttata caactcaata actagctcaa ttattttccc gattgcccag acaaaaatta
     2881 gcggggatat cggtgtcatc aacacgataa acagaatgag aaacaaaaat tctgtcgctc
     2941 tactttttcg cggatattct tttctgaata atgtaggcac atcactctcc tttgttgctc
     3001 ctcaaaattt tatgccctgg cgcaaaagca cgcgttttgt ctttgtttat tcgccagcca
     3061 tccttgcgcg cctcttttgc acagccagcc catgacgtac cgatatactc accgaaatct
     3121 ggcgtttgat atttaccatt tgtacactgg cgacaatcac agtagagatg catggtgtaa
     3181 cttgcggcaa tagccatatc cccctccttt accctgaagc atggcgtcgc tccgctctat
     3241 accatccagc gcgattcgca gtgcctgaat tgtggtagtg ctatcgtttg gggctattcc
     3301 atatcgctcg aatacagcta aatggttgcg cataatctca ggcgtaagct ctttgtaagc
     3361 ataagcaaga ggctctgatg cattatccgg cacaaccgac gcaggcgcgg cagcataaac
     3421 aggaataacg tccgcttgct ctttattgct ttcatccgtt aaagcccaga ataatttccc
     3481 ggccggatgt ttgaaaatat aagcaactgg ttctgcacta tcagcttcgc gccgcttctg
     3541 tagctctgct gccatcgctc tcacgacttc aactggtgcc cttgcagcaa actccatgtt
     3601 ggtgatcagc tcattaagaa attgctcgct gggatactgt ttcttatcgg ttatagtggt
     3661 catatcactc tcctttgatg cgaatgccag caagccagtt tcttatgccg atatattcag
     3721 cgttcctgaa accgcttttt acatatataa atggcaatcg aagattgtga ccattgactg
     3781 ctaggtaatc tttacaaccc tgttcggtga aacagcaggt aacgaattca tcaatatctt
     3841 tcacagcaac gcgccgccat ttttctggtg gctcccgaaa gttttcatga agtagttcga
     3901 gacgacgact ttggagttta ttggcttcat tgccatcttc atcaacccag acaatccggt
     3961 catagtcata atcagcatca acaacgattt cgcgcttttg atacacacaa aacataggat
     4021 ctgacgttat tcgattatcc tgtgttcgaa tattttcacc gatgatgcca aacgaatctg
     4081 gtgcagattt tgtctgcatc tcttcgatac gttcagccat cgcagcacac tcttcaaagt
     4141 tgcttaatgc ttttcgctcc cattcggcgc attgtttttc cagttctgct atgtgcttac
     4201 ttccatccga gataacgccc tcgtaatact cacgctgctc gttgagtttt gattttgctg
     4261 cttcaagctc aacgcgcagc ttccctaccg ttagcgcaat atcctcgttc tcctggtcgc
     4321 ggcgtttgat gtattgctgg tttctttccc gttcatccag cagtgccagc gcaacatttg
     4381 gattaaaggc agcaataaat tcagcgtttg cgtaagcctg agcatctgtt tcaaccaggc
     4441 agttaacatg acattctgca atcacgccac cgggttctcc tttccatttt tggcaaacaa
     4501 aaactcctgt taaattgccg tgttggttaa cagatgtatg ccctacgatg tagcttcctt
     4561 tagttgcttt ctctgccttt tcacgcagtg cctgataatt aatttcgctc acttcgaacc
     4621 tctctgttta ctgataagct ccagatcctc ctggcaactt gcacaagtcc gacaaccctg
     4681 aacggccagg cgtcttcgct catctatggg atcgccacac tcacaacaat gagtggcaga
     4741 tatagcctgg tggttcaggc ggcgcatttt tattgcggtg ttgcgctgta attcttcaat
     4801 ttctgatgct gaatcaatga ggtctgccat ctttcattaa tccctgaatt gttggttaat
     4861 acgcttgagg gtgaatgcga ataataaaaa aggagcctgt agctccctga tgattttgct
     4921 tttcatgttc accgttcctt aaagacgccg tttaacatac cgattgccag acttaagtga
     4981 gtcggtgtga atcccatcag cgttaccgtt tcgcggtgct tcttcagtac gctacggcaa
     5041 atgtcatcga cgtttttatc cggaaactgc tgtctggctt ttttgatttc agaattagcc
     5101 tgacgggcaa tgctgcgaag ggcgttttcc tgctgaggtg tcattgaaca agtcccatgt
     5161 cggcaagcat aagcacacag aatataaagc ccgctgccag aagaatgcat tcagtggttg
     5221 tcatacctgg tctctctcat ctgcttctgc tttcgccacc atcatttcca gcttttgtga
     5281 aagggatgcg gctaacgtat gaaattcttc gtctgtttct actggtattg gcacaaacct
     5341 gactccaatt tgagcaaggc tatgtgccat ctcaatgctc gttcttaact caacaggaga
     5401 tgctttgtgc atacagcccc tcgtttatta tttatctcct cagccagccg ctgggctttc
     5461 agtggatttt ggataacaga aaggccggga aatacccagc ctcgctttgt aacggagtag
     5521 acgaaagtga tcgtgcctac gcggatatta tcgtgaggat gcttcatcgc cattgctccc
     5581 caaatacaaa accaatttca gccagtgcct cgtccatttt ttcgatgaac tccggcacca
     5641 tctcgtcaaa actcgccatg tacttttcat cccgctcaac cacgacataa tgcagtcctt
     5701 cacgcttcat acgcgggtca tagttggcaa agtaccaggc atcttttcgc gtcacccaca
     5761 tgctgtactg cacctgggcc aggtaagccg attttatggc ctcgaaacca ccgagccgga
     5821 atttcatgaa atcccgggag gtaaacgggc atttcagttc aaggccgttg ccgtcactgc
     5881 ataaaccatc gggagagcag gctgtacgca tactttcgtc gcgatagata atcggggatt
     5941 cagtaacatt cacgccggag gtgaactcaa acaggactct ggcgtcgttc tcgtattgtt
     6001 ttccccaggc cagtgctttg gcattaactt ccggagccac accggtgcaa acctcagcaa
     6061 gcagggtgtg gaagtaggac attttcatgt caggccattt ctttcctgaa cggggttttg
     6121 ctatcacgtt gtgaacttct gaagctgtaa tgacgccgag ccgtaatttg tgccacgcat
     6181 catccccctg ttcgacagct ctcacgtcga tcccggtacg ctgcaggata atgtccggtg
     6241 tcatgcagcc accttctgtt cagaggcttt ctgtttcagg aatccaagag ctttcactgc
     6301 ttcggcctgt gtcagttctg acgatgcgcg aatgtcgcgg cgaaatatct gggaacagag
     6361 cggcaataag tcgtcatccc atgttttgtc cagggcaatc agcagagtgt taatctcctg
     6421 catggtttca tcgttaaccg gagtgatgtc gcgttccggc tgacgttctg cagtgtatgc
     6481 ggtattttcg acaatgcgct cggcttcatc cttgtcatag ataccagcaa atccgaaggc
     6541 cagacgggca cactgaatca tggctttatg ccgtaacatc cgtttgggat gcgactgcca
     6601 cggtccggtg atttctctgc cttcgcgggt tttgaatggt tcgcggcggc attcatccat
     6661 ccactcggta acgcagatcg gatgattgcg gtctttgcgg taaatccggc atgtgcagga
     6721 ttcattgtcc tgctcaaagt ccatgccatc aaactgctgg ttttcattga tgatacggga
     6781 ccagccatca acgcccacca ccggaacgat gccgttctgc ttatcaggaa aggcgtaaat
     6841 ttctttcgtc cacggattaa gaccgtactg gttggcgacg atcagcaatg cgatgaactg
     6901 cgcatcgctg gcatcacctt taaatgccgt ctggcgaaga gtggtgatca gttcctgtgg
     6961 gtcgacagaa tccatgccga cacgttcagc cagcttccct gccagcgttg cgagtgctgt
     7021 actcattcgt tttatacctc tgaatcaata tcaacctggt ggtgagcaat ggtttcaacc
     7081 atgtaccgga tgtgttctgc catgcgctcc tgaaactcaa catcgtcatc aaacgcacgg
     7141 gtaatggctt ttttgctggc cccgtggcgt tgcaaatgat cgatgcatag cgattcaaac
     7201 aggtgctggg gcaggccttt ttccatgtcg tctgccagtt ctgcctcttt ctcttcacgg
     7261 gcgatctgct ggtagtgacg ctcccagctc tgagcctcaa gacgatcctg aatgtaataa
     7321 gcgttcatgg ctgaactcct gaaaatggct gtgaaaatat cgcccgcgaa atgccaggct
     7381 gattaggaaa acaggaaagg ggggtagtga atgcttttgc ttgatctcag tttctgtatt
     7441 aatatccatt ttttataagc gtcgacggct tcacgaaaca tcttttcatc gccaataaaa
     7501 gtggcgatag tgaatttagt ctggatagcc ataagtgttt gatccattct ttgggactcc
     7561 tggctgatta agtatgtcga taaggcgttt ccatccgtca cgtaatttac gggtgattcg
     7621 ttcaagtaaa gattcggaag ggcagccagc aacaggccac cctgcaatgg catattgcat
     7681 ggtgtgctcc ttatttatac ataacgaaaa acgcctcgag tgaagcgtta ttggtatgcg
     7741 gtaacgccgc gctcaggcgg ctttgatagt catatcatct gaatcaaata ttcctgatgt
     7801 atcgatatcg gtaattctta ttccttcgct accatccatt ggaggccatc cttcctgacc
     7861 atttccatca ttccagtcga actcacacac aacaccatat gcatttaagt cgcttgaaat
     7921 tgctataagc agagcatgtt gcgccagcat gattaataca gcatttaata aagagccgtg
     7981 tttattgagt cggtattcag agtctgacca gaaattatta atctggtgaa gtttttcctc
     8041 tgtcattacg tcatggtcga tttcaatttc tattgatgct ttccagtcgt aatcaatgat
     8101 gtattt
//
LOCUS       DBFIFS010000194         8076 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.194, whole genome shotgun sequence.
ACCESSION   DBFIFS010000194 DBFIFS010000000
VERSION     DBFIFS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.194"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            88..564
                     /locus_tag="N7A07_003832"
     CDS             88..564
                     /locus_tag="N7A07_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU2478413.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            561..2684
                     /locus_tag="N7A07_003833"
     CDS             561..2684
                     /locus_tag="N7A07_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2478414.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2681..2893
                     /locus_tag="N7A07_003834"
     CDS             2681..2893
                     /locus_tag="N7A07_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478415.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2893..4395
                     /locus_tag="N7A07_003835"
     CDS             2893..4395
                     /locus_tag="N7A07_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2478416.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4340..6364
                     /locus_tag="N7A07_003836"
     CDS             4340..6364
                     /locus_tag="N7A07_003836"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU2478417.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            6452..6778
                     /locus_tag="N7A07_003837"
     CDS             6452..6778
                     /locus_tag="N7A07_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGU2478418.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            6771..7052
                     /locus_tag="N7A07_003838"
     CDS             6771..7052
                     /locus_tag="N7A07_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU2478419.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            7055..7678
                     /locus_tag="N7A07_003839"
     CDS             7055..7678
                     /locus_tag="N7A07_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2478420.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            7691..>8076
                     /gene="gpU"
                     /locus_tag="N7A07_003840"
     CDS             7691..>8076
                     /gene="gpU"
                     /locus_tag="N7A07_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU2478421.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
BASE COUNT         1870 a         1927 c         2531 g         1748 t
ORIGIN      
        1 ttttttgttc gacatcttta tatggcggca tttctttaca aaaaaacaga gtcacttctg
       61 ttctgcggtt tatgtggagg gatgtaaatg gacggcgagc tgaaaaatat gaagttaaat
      121 attaatcaac tggcagccct ttcaggtctg caccggcaga ctgttgccgc cagaatggcg
      181 gatgttcctc ttgcaccagg cagtaatgaa aagaaaaaac tgtatctcct gacggatttg
      241 ataacttcgt tgctggaaaa accaccatct tccgaagatg aggatatgga tcctcacgct
      301 cgtaaggcat ggtatcagtc cgagcgcgag cgtcttaaat ttcagcatga aactgttcag
      361 cttgtgccag tcagtgatgt caggcggtcc ttttctgtcg tggtgaaagc gatagttcag
      421 gtactggaaa cctggcctga ccggctggag agggacaggg ggtggactgc atcacaactg
      481 aatgaagtac agattgtggt tgatgagatc cgcgacacac tggaaaaggc agtcattgac
      541 tgttgtgatg aggccgatat gtgaatcagg tgaacgagag ccatagccgc gcatccgata
      601 tctggcgcga agtggcctcg ctgtttcgcc cacccagccg gttaccagta gcggaagcca
      661 tcaggcgtta tatgcgggtt ccacggggag ccaatacttc cggtccgtgg gagtcatcgc
      721 tgacgcccta tatgattgac cccattaata cattatcagc ccgtgaatat gacgcggtgg
      781 tgtttgtggg acctgcgcga accgggaaaa ccgaagggct gattgatggc tggattgtgt
      841 acggcatcat ctgtgatccg gcggatatgc tggtggtgca gatgactgag acgaaggcgc
      901 gtgagcattc cagaacgcgt ctttccagga cgtttcgcca cagtccggag gtcagcaagc
      961 gcctcagtcc ttcccgtaat gacaacaacg tccacgataa aatgtttctt gacggctcct
     1021 tcctgaagat tggctggccg tcgatcaccg tcttttcctc ttcggattac cgtcgtgtgg
     1081 cgctgacgga ttatgaccgt ttccctgaaa atgtggacgg ggaaggggat gccttcacgc
     1141 tggcctcaaa gcgtaccacc acctttatgt cctcggggat gaccctggtc gagagttcac
     1201 cggggcggga tatcaccgat accaaatggc gttgtggtgg cgcacatgag gcaccgccaa
     1261 caacggggat cctgtcactg tataaccggg gagaccgccg ccggtggtac tggccgtgtc
     1321 cgcactgcgg ggaatatttt cagccggtga tggataacat gaccggatac cggaataacc
     1381 cggattttgt ggctgccggt caggctgccc gtctgatgtg tccgcattgt cgcgggctga
     1441 ttgcccctga gcagaaacgc gaactgaata accaggggat ctggcttcgt gaaggtgaac
     1501 gggcggcggc ggacggcagt atcaccggaa cgccacgaaa ctcccggatt gcgtcatttt
     1561 ggatggaggg gccagctgcg gcgtttcaga cctgggaaca actgattttt aaactgctgg
     1621 cggcagaaga agagtatgag cgaaccggca gtgaagagac cctgaaagcg gtggtgaaca
     1681 ccgatatcgg acgaccctat ctgccccgtt cagccacgga acagcgtaaa agtgaactgc
     1741 ttgaacagcg tgccgagccg tttccccggc gatctgtgcc ggatggtgtg cgttttattg
     1801 aggcaacggt tgacgtacag ggcggtaaaa atcgccgttt tgttgtgcag atcaccggat
     1861 acggagagca gggggaacgc tggattgttg atcgctacaa catccggcat tcactgcgct
     1921 gcagtcccaa cggtgaaagt ctgccggttg atccggcggc atatccggag gactgggatt
     1981 tgttgctgac ggatgtgttc cataaaacat ggccgctggc ttctgatccg gatgtgcgca
     2041 tgcgtctgat ggccatggcg gtggatacgg gaggggaagc cggggtgaca gataacgcct
     2101 atcgtttctg gcgtcgttgc cggagtgacg gactgggcaa cagggtgttt ctgttcaagg
     2161 gggatggact tcgccgtgac aggctgatta accgaacctt cccggataat accggcagaa
     2221 gtgcccgccg tgccagagcc agtggcgatg tcgcgctgtg gctggttcag acggatgcgt
     2281 ttaaggatcg tgtaaataat gccctgtggc gtgacacacc agggccgaac tatatccact
     2341 ttcccgactg gctggggcgg tggttttacg atgagctgac ctatgaagag cgcggcagtg
     2401 acggaaaatg gcgaaaaccg ggcaggggcg ctaacgaagc gtttgacctg ctggtttatg
     2461 cggatgcgct tgccgttctg catggttacg aaaagatccg ctggccctcc gcaccggact
     2521 gggcacagcg ggaaacgtgg ctcgtcttcc cgcaggagcg ttctggtgaa acggtatccc
     2581 cggaactgac ggccggggca gaaaaacgcc gtcgccggaa gaaaaaactg cggacggagc
     2641 gtgcggaaga taatccatgg ataacatcag gaggctggtt gtgagcacag aagaagccag
     2701 agaaatgata cagcggtacc gtgaagcgga aatggccgta ctggagggaa agtctgtcat
     2761 cttcaacggg cagcaactga cgctggaaag cctttctcag atccgcgccg gacgtcagga
     2821 gtgggaacgc aggcttgccg cgatggtgag ccgcaggcgg ggaaaaccgg gatttaaact
     2881 ggcgaggttt taatggcaat tattgatgat gtgatcggcg tgttttcccc cgggtggaaa
     2941 gcagccagac tgcgttcaag ggcgttaatc atggcctatg aggcggtgaa accgacccgg
     3001 acacataaag cccggcggga aaatcgctct gctgatcagc tcagtaaata cggtgcggtt
     3061 tccctgcggg agcaggcccg ttttctggat atcaatcatg acctggtgat tggtgtgttt
     3121 gacaagctgg aagagcgggt gattggtgcc aggggaatta ttgtggagcc tcagccatta
     3181 cgaaaaaacg gggaaatggc ggctgagctg gctgcggata tccgccgttt gtgggctgaa
     3241 tggtccgtga gtccggatgt gacagggcag tatacccgtc ctgtgcttga acgtttactg
     3301 ctgcggacct ggctgcggga tggtgaagtg tttgcgcaga tggtcagtgg tgcgggaaac
     3361 ggtctggaac ggacggcggg agtgccattc tggcttgagg cgatggagcc ggattttgtt
     3421 cccatgcgca ctgatgaatc cgccggactg aatcaggggg tttttcttga tgagtgggga
     3481 agaccgaaaa aatatctggt ttataaaaat tatccggtca gcggccggca gagtgatacg
     3541 aaagaaatcg ctgccggaaa aatgatccac ctgaagttca ctcgtcgtct gcatcagacg
     3601 cgaggctcat ccatgttatc gggggtgctg atgcggatca gtgcccttaa ggagtatgag
     3661 gatgcggaac tgacagcggc gcgtattgct gcggcgctgg gactgtatat ccgtaaaggt
     3721 gacggacagg actatgaaga tccggggatc aaagagaccg agcgggaagt ccatatcacc
     3781 ccgggtatta tttatgacga tttgcgcaag ggcgaggata tcggcatggt caaatctgac
     3841 cgtcccaatc ccaaccttga aactttccgc aacggccagt tgcgtgcagt ggcagcaggc
     3901 agtcgtctga gtttttccag tgcggcgcgt aactataacg gcacctacag cgcccagcgg
     3961 caggagctgg tcgagtccac ggatggttac ctgatcctgc aggactgttt tattggcgcg
     4021 gtaacccgcc cggtgtaccg gacatggctg aatatggtgg ttgcggcagg tctgctgaaa
     4081 attccggcgg atgtggagat gaaaacgcta tataacgcga cgtattccgg tccggtgatg
     4141 ccgtggatcg acccggttaa ggaagctgaa gcctggagaa ttcagatccg gggtggtgca
     4201 gcgacagaat ctgactgggt gcgtgccggt gggcgcaatc cggatgaggt caaacgtcgc
     4261 cgcaaggctg aaattgatga aaacagcaga ctggggctgg tctttgatac tgaccccgtc
     4321 aacgacaaag gaggcaacag tgccggaact gaacgacagt atcagcgcga caccgaaagc
     4381 cagcatgaag aataaatcct ggttcaggat gcaagctggg gggccgggtg acgcggatat
     4441 ttatatttat gacgagattg gtttctgggg agttaccgcg aagcagtttg tcagcgaact
     4501 gaatgcactg ggtgatatca cccacattaa tctccatatc aattcaccgg gtggcgatgt
     4561 ctttgaaggc atcgccattt ttaatgccct gaaaaatcag ggggcgacca ttaccgtgta
     4621 tgtggatggc gttgccgcct cgatggcatc tgtgattgcg atggccggtg atacggtcat
     4681 tatgccggaa aatgccttca tgatgatcca taagccatgg ggattcagtg gcggggatgc
     4741 tgaggatatg cgcagttatg ccgatttgct ggataaagtc gaatcggtac tgttgccagc
     4801 ctatgcgcag aaaaccggaa aaaccaccga tgaaattgcc gccatgctgg cggatgaaac
     4861 ctggatgtcc ggtgccgaat gtctggcaca cggatttgct gaccaggtga cacccgctgt
     4921 tgaggcaatg gcatgtattc agtcaaaacg tacagaggaa tttaaaaaga tgccggaatc
     4981 catccgaaac atgattactc cgccacgcaa cagtgccccg cgtgatacca cagtgacaat
     5041 ccctgcaccg gcggtaacag aaccatcacc ggtaccggca gtgtctgatg aggcgaccat
     5101 tcgcgcccgc gttatggctg agcagaaagc ccgcatgtca ggcattaacg atctgtttgc
     5161 catgttcggc ggtcgctatc agacgcttca ggcacagtgc gtggctgatc ctgactgttc
     5221 gctggaaatg gcccgtgaac gtctgctgaa tgaaatgggc aaggagtcct cgccgaccaa
     5281 caaaaataca ccggcccata tttatgccgg aaacggcaat tttgtggggg acgggatccg
     5341 ccaggcgatg ctggcccgtg ccggatttga aaatgtcgag aaggataacg cctataacgg
     5401 gatgaccctg cgtgaatggg ctcgcatgtc actgacggag cgcggtattg gggtggccag
     5461 ttataacccc atgcagatgg tcgggctggc gctgacgcac agcacctctg attttggcaa
     5521 tattctgctg gatgtgtcga acaaggggct gatccagggc tgggaggaat cagaagaaac
     5581 cttccagaag tggacccgta agggacgcct gtcagacttc aaaacagcgt atcgcgtggg
     5641 gatgggcggt tttggttctc tgcgccaggt tcgtgagggg gcggagtata aatacatcac
     5701 cacctcagat cgcaaggaga ccattgcact ggccacttac ggggagattt tctccatcac
     5761 ccgccaggcc attatcaatg atgatctgaa tatgctggtg gacgtgccga tgaagatggg
     5821 gcgtgcggcg aaggcaacga ttggtgacct ggtctacaag gtgctgacgg ataacccgaa
     5881 actgtccgac ggtaaggcgc tgttccatgc cgatcacaaa aatattgcca ccggggggat
     5941 ctccgtttcc ggactggatg cggcccgtca gatgatgcgc ctgcagaaag aaggcgatcg
     6001 tgccctgaat atccgtccgg cctttatgct ggtaccggtg gcactggaga cggtggcgaa
     6061 ccagaccatc aaatcggcca gtgtgaaagg ggcggatgca aacgccggtg tcattaaccc
     6121 tatccagaac tttgctgagg tgattgcaga agcgcgtctt gatgcggcag acccgaaaac
     6181 ctggtatctg gcggcggcac agggcactga caccattgaa gtggcctggc tggatggtgt
     6241 ggacacgcca tacattgatc agcaggaagg tttcaccact gacggcattg ccacaaaaat
     6301 ccgtattgat gccggagtgg caccacttga ctggcgcggg ctggtgcgtt cgtcggtggc
     6361 ctgataaccg cgttatcaca atcactgccc gaaagggctt tttttatgcc tgaaaaacag
     6421 ccccacaggg gctgtccgga gaaacagcat tatggcgaaa aattttgtac aggacggtac
     6481 caccattgaa ctggtgaatg ccggagatca gaccatcctg agcggtgctg cggtggtggt
     6541 cggcagtatg gtggccgtgg ccattaccga tattcctgcc ggtgaggccg gtgacggttt
     6601 tgccgaaggc gtgttcctgc tgcccaaaca gtctgctgac gacattcagt ccggcgcggt
     6661 ggtttatctg aaggacgggg ttgtgcagct ggctgcagac ggtgcggtgg cagcgggggt
     6721 agcctgggaa aatgcccctg caaacagcgc cactgtggcg gtaaaaatca atgtctgatc
     6781 tgtttacgcg aatgtgttgc cggatggacg tggcgaccgt tcgggtgatg ggcaaacagg
     6841 cggagattaa cggcgtcgtg tacgacgtga tgccggagga agagtccgcg gagatggggg
     6901 cgctttcggg cagccagttg tcactggtgg tgttttcagc ccggtaccgt ccggcccgtc
     6961 atgatgttgt tgtgtttgcg gggcgcacac tgacggtgac ccgttatgac acgtacaacg
     7021 gtaaaccccg gatttttgtc gaacaggaat gagtatggca ataaaaggtc tggcgcaggc
     7081 catgaaaaat ctggatgcaa ttgatcgccg tgccgttccc cgggcctctg ccacgacact
     7141 gaaccgagtg gcgggggcca ttattgcgaa aacggcctct tcagttgcca gggagctggc
     7201 cgttccccgt cgtcttatcc gtgcccgcat ccggttaagt ccggcacgac cggataaggt
     7261 ttacgcaaag gtttacatca ataccggcaa cctgcccgcc atcaaactgg gggaggcccg
     7321 cgttcgactt tcccgcagaa aacggagaaa gaaaggacag cgtgcggccc tgaaaggggg
     7381 cggcagtgtg ctgattgtgg ggaaaagacg gatcccggac gcctttatca cccggctggc
     7441 taacggacgc tggcatgtga tgcagcgtat gccgtgggca tcatcatcca ccggcgcgga
     7501 cagcaaaggg aggccgaaac gccaccgtct gccgatcgaa gtggtgaaga ttacgactgc
     7561 cggaccgctg gcagaaacct ttgaacgtga acgggaccgg atgtaccggg aaaaattacc
     7621 ggcgcagatg atgaaagcca tgacgcatca gttacgcctg gtgctgaaaa gaaaatgact
     7681 gggagggtgt atgaaacacc gtgaaatacg ggcggcagtt ctgtctgccc tgaaagaaaa
     7741 tatttctgag agggtgagct ggtttgacgg tcgcccggtt tttattgatg aacaggaact
     7801 gcctgctgtt gctgtttacc tgacagatgc gtctgctgct gacgagttcg ttgatgaggg
     7861 aacctgggag gcgacactgc atattgaggt ttttctcagg gcaaaagaac cggactcggc
     7921 actggatatg tggatggaag aaaaaattct tcctgcgctg gaggcagttc ccgggctcag
     7981 tgcattactg ctgaagatga atcttcaggg gtatgactac cgccgggatg atgagtttat
     8041 gatgtgggga tcggcagatc tcctgtggaa aattac
//
LOCUS       DBFIFS010000195         8062 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.195, whole genome shotgun sequence.
ACCESSION   DBFIFS010000195 DBFIFS010000000
VERSION     DBFIFS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.195"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            230..370
                     /gene="ymiA"
                     /locus_tag="N7A07_003841"
     CDS             230..370
                     /gene="ymiA"
                     /locus_tag="N7A07_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU2478422.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            373..540
                     /gene="yciX"
                     /locus_tag="N7A07_003842"
     CDS             373..540
                     /gene="yciX"
                     /locus_tag="N7A07_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU2478423.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            654..749
                     /gene="ymiC"
                     /locus_tag="N7A07_003843"
     CDS             654..749
                     /gene="ymiC"
                     /locus_tag="N7A07_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU2478424.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            913..3588
                     /gene="acnA"
                     /locus_tag="N7A07_003844"
     CDS             913..3588
                     /gene="acnA"
                     /locus_tag="N7A07_003844"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU2478425.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDCEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3652..4242)
                     /gene="ribA"
                     /locus_tag="N7A07_003845"
     CDS             complement(3652..4242)
                     /gene="ribA"
                     /locus_tag="N7A07_003845"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU2478426.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4412..5176
                     /gene="pgpB"
                     /locus_tag="N7A07_003846"
     CDS             4412..5176
                     /gene="pgpB"
                     /locus_tag="N7A07_003846"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU2478427.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5325..5633
                     /gene="lapA"
                     /locus_tag="N7A07_003847"
     CDS             5325..5633
                     /gene="lapA"
                     /locus_tag="N7A07_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU2478428.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            5640..6809
                     /gene="lapB"
                     /locus_tag="N7A07_003848"
     CDS             5640..6809
                     /gene="lapB"
                     /locus_tag="N7A07_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU2478429.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7002..7739
                     /gene="pyrF"
                     /locus_tag="N7A07_003849"
     CDS             7002..7739
                     /gene="pyrF"
                     /locus_tag="N7A07_003849"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU2478430.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7739..>8062
                     /gene="yciH"
                     /locus_tag="N7A07_003850"
     CDS             7739..>8062
                     /gene="yciH"
                     /locus_tag="N7A07_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU2478431.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1971 a         1883 c         2156 g         2052 t
ORIGIN      
        1 ttttatccct catatttata gggtaaatta cctgtaaagt agtgtctaat tacccgcctc
       61 gcaaattcca gttttcaaat atctatttca ggtcaaaaga ttgaattatt cactggagac
      121 gattcgtaaa ttcgctggat ttttgactaa agtttcttta ggatttgtct catcgtatga
      181 ttaattacac caatcgttca acgtcttgct gataagtgat ggcgattata tgaggttagc
      241 aatgccttct ggaaatcagg aaccccgccg cgatcctgaa ttaaaacgta aagcctggct
      301 ggcggttttt cttggttctg cacttttctg ggtggttgtc gcactgctga tttggaaagt
      361 gtggggataa ctatggttgg tcaggagcaa ctggagtcgt caccattatg ccagcatagt
      421 gacaatgaac ccgaaacgaa acgggaatgt tccgtcgtta ttccagacga ctggcaacta
      481 acatcgcagc agcaagcctt tatagaactg tttgctgaag atgatcagcc gaaacaataa
      541 ttatcatcat tattattacc catttttaat gaattaaagg gcttttaata caccgcagca
      601 ataacaggtt gagttatctc aacacaaagt aataaccgtt aagggtgtag cctatgatca
      661 acacaaatat gaaatattgg tcctggatgg gtgcattttc tctgtcgatg ctcttctggg
      721 ccgaactcct ctggatcatt actcactgat ccttgacccc gctgcggcgg ggttgtcatt
      781 tgctttgcca caaggtttct cctcttttat caatttgggt tgttatcaaa tcgttacgcg
      841 atgtttgtgt tatctttaat attcaccctg aagagaatca gggcttcgca accctgtcat
      901 taaggaggag ctatgtcgtc aaccctacga gaagccagta aggacgcgtt gcaggccaaa
      961 gataaaactt accactacta cagcctgccg cttgctgcca aatcactggg cgatatcacc
     1021 cgtctaccca agtcactcaa agttttgctc gaaaatctgc tgcgctggca ggatggtaac
     1081 tcggttaccg aagaggatat ccacgcgctg gcgggatggc tgaaaaatgc ccatgctgac
     1141 cgtgaaattg cctaccgccc ggcaagggtg ctgatgcagg actttaccgg cgtacctgcc
     1201 gttgttgatc tggcggcaat gcgcgaagcg gttaaacgcc tcggtggcga tactgcaaag
     1261 gttaacccgc tctcaccggt cgacctggtc attgaccact cggtgaccgt cgatcgtttt
     1321 ggtgatgatg aggcatttga agaaaacgta cgcctggaaa tggagcgcaa ccacgaacgt
     1381 tatgtgttcc tgaaatgggg aaagcaagcg ttcagtcggt ttagcgtcgt gccgccaggc
     1441 acaggcattt gccatcaggt taacctcgaa tatctcggca aagcagtgtg gagcgaattg
     1501 caggactgtg aatggattgc ttatccggat acactcgttg gtactgactc gcacaccact
     1561 atgatcaacg gccttggcgt gctggggtgg ggcgtgggtg gcatcgaagc agaagccgca
     1621 atgttaggcc agccggtttc catgcttatc ccggacgtag tgggctttaa acttaccgga
     1681 aaattacgtg aaggtattac cgccacagac ctggttctca ctgttaccca aatgctgcgc
     1741 aaacatggcg tggtggggaa attcgtcgaa ttttatggtg atggtctgga ttcactaccg
     1801 ttggcggatc gcgccaccat tgccaatatg tcgccagaat atggtgccac ctgtggcttc
     1861 ttcccaatcg atgctgtaac cctcgattac atgcgtttaa gcgggcgcag cgaagatcag
     1921 gtcgagttgg tcgaaaaata tgccaaagcg cagggcatgt ggcgtaaccc gggcgatgaa
     1981 ccgattttta ccagtacgtt agatctggat atgaatgacg ttgaagcgag cctggcaggg
     2041 cctaaacgcc cacaggatcg cgttgcactg cccgatgtac caaaagcatt tgccgccagt
     2101 aacgaactgg aagtgaatgc cacgcataaa gatcgccagc cggtcgatta tgttatgaac
     2161 ggacatcagt atcagttacc tgatggcgct gtggtcattg ctgcgataac ctcgtgcacc
     2221 aacacttcta acccaagtgt gctgatggcc gcaggcttgc tggcgaaaaa agccgtaact
     2281 cttggcctca agcggcaacc gtgggtcaaa gcgtcgctgg caccgggttc gaaagtcgtt
     2341 tctgactatc tggcaaaagc gaaactgaca ccgtatctcg acgaactggg gtttaacctt
     2401 gtgggatacg gttgtaccac ctgtattggt aactctgggc cgctgcccga tcctatcgaa
     2461 acggcaatca aaaaaggcga tttaaccgtc ggtgcagtgc tgtccggcaa ccgtaacttt
     2521 gaaggccgta tccatccgct ggttaaaact aactggctgg cctcgccgcc gctggtggtt
     2581 gcctatgcgc tggcgggaaa tatgaatatc aacctggctt ctgagcctat tggtcatgat
     2641 cgtaaaggcg atccaatata tctgaaagat atctggccat cggcacaaga aattgcccgt
     2701 gcggtagaac aagtctccac agaaatgttc cgcaaagagt acgcagaagt ctttgaaggc
     2761 actgcagagt ggaaagaaat taacgtcgca cgatccgata cctacggatg gcaggaggat
     2821 tcaacctata ttcgtttatc gcctttcttt gatgaaatgc aggcaacgcc agcacctgtg
     2881 gaagatattc acggtgcgcg gatcctcgca atgctggggg attcagtcac cactgaccat
     2941 atctctccgg cgggcagtat taagcccgac agccctgcgg gtcgatacct acaaggtcgg
     3001 ggtgttgagc gaaaagactt taactcctac ggttcgcggc gtggtaacca tgaagtgatg
     3061 atgcgcggca ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg cgttgaaggg
     3121 gggatgacgc ggcatttacc tgacagcgac gtagtctcta tttatgatgc tgcgatgcgc
     3181 tataagcagg agcaaacgcc gctggcggtg attgccggga aagagtatgg atcaggctcc
     3241 agccgtgact gggcggcaaa aggtccgcgt ctgcttggta ttcgtgtggt gattgccgaa
     3301 tcgtttgaac gaattcaccg ttcgaattta attggcatgg gcatcctgcc gctggaattt
     3361 ccgcaaggcg taacgcgtaa aacgttaggg ctaaccgggg aagagaagat tgatattggc
     3421 gatctgcaaa atctacaacc cggcgcgacg gttccggtga cgcttacgcg cgcggatggt
     3481 agccaggaag tcgtaccctg ccgttgtcgt atcgacaccg cgacggagtt gacttactac
     3541 cagaacgacg gcattttgca ttatgtcatt cgtaatatgt tgaagtaaca agtatttgct
     3601 tgccggttat tttgcttccg gcaagcaaat taattacaca atgcaagagg gttatttgtt
     3661 cagcaaatgg cccattttct cggctttggt atcgagataa tgttcgttat tggggttacg
     3721 acctacaatc aatggtacgc gttcaacaat attaatcccc gcttcggtca gaatttcgac
     3781 ttttttcggg ttattggtta acaagcggac ttcattgacg ccaaggagtt tgaacatatc
     3841 agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt tagcctctac
     3901 agtatcgtaa ccttgatcct gcagtgcgta agcgcggatt ttattcagca gaccaatgtt
     3961 acgaccttcc tgacggtgat acagcaaaat accacggcct tcctcggcaa tttgcgtcaa
     4021 tgccgcttcg agctggaagc cacaatcgca gcgcaagctg aacagggcgt caccggtcag
     4081 acattcggaa tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc catagactag
     4141 cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc accatcagga aatcgcccca
     4201 tggggttggc agtttggctt ctgccacacg tttaagctgc atgaaattct ccagataatg
     4261 ctggttctgt attggcttat tttgccataa cgagaagggg ttcacctaat cacgacgcgt
     4321 cgatcgttca cggaatggca caaatctgtc aatttttcct ggaactggca ttttcagtta
     4381 tgattgtggg acttatcaaa aaggagaggc catgcgttcg attgcaagac gtaccgcagt
     4441 gggagctgca ctattgcttg ttatgccagt agccgtatgg atttctggct ggcgttggca
     4501 acctggagaa caaagttggc tactaaaagc ggctttttgg gttactgaaa ctgtcaccca
     4561 gccctggggc gtcattacac atttgatttt attcggctgg tttctctggt gtctgcgttt
     4621 tcgcattaag gctgcctttg tattatttgc cattctggcg gccgcaatcc ttgtgggaca
     4681 aggcgttaaa tcctggatca aagacaaagt ccaggaacca cgaccttttg ttatctggct
     4741 ggaaaaaaca catcatattc cggttgatga gttctacact ttaaagcgag cagaacgcgg
     4801 aaatctagtg aaagaacagt tggctgaaga gaaaaatatt ccacaatatt tgcgttcaca
     4861 ctggcagaaa gagacggggt ttgcctttcc ttccggtcac acgatgtttg ctgccagttg
     4921 ggcactgctg gccgttggtt tgctgtggcc gcgtcggcga acgttaacca ttgctatctt
     4981 gctggtctgg gcaacgggag tcatgggaag ccgcctgctg ctcgggatgc attggccacg
     5041 cgatctggtg gtagctacgt tgatttcgtg ggcactggtg gcggtggcaa cgtggcttgc
     5101 gcaacgaatt tgcgggccat taacaccacc tgcggaagaa aatcgcgaaa tagcgcaacg
     5161 agaacaagaa agttaacgct ggttgatttt ccgaatttag cccttaaatc atcaacaatg
     5221 cgcgtggatg ccatttcgca gacggcgcga aaatggtact ttaaagggct attgcggtaa
     5281 gttgaccata atttattcgc tctaaccaca taacgggaag taatgtgaaa tatttactca
     5341 ttttcttact ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg cagaacgatc
     5401 aacaggtgac gtttaattat ctgttagcgc aaggggagta ccgtatttcc acattgctgg
     5461 cggtattgtt tgctgcgggt tttgctatcg gttggttgat ttgtggcctg ttctggctgc
     5521 gagttcgtgt ttccctggtg cgcgctgaac gtaaaataaa gcgactggaa aaccagcttt
     5581 cacccgcgac tgacgtggct gtagtgcagc actcgtcagc ggcgaaggaa taactttcta
     5641 tgctggagtt gttgtttctg cttttgcctg tagccgctgc ctatggctgg tatatgggcc
     5701 gcagaagtgc gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt gattacgtag
     5761 cgggggttaa cttcctgctt agtaatcaac aggataaagc ggtagacctg tttctcgata
     5821 tgcttaaaga ggatacaggc accgttgaag cccaccttac gctcggaaac ctgttccgtt
     5881 cgcgtggcga agttgatcgc gctattcgca tccatcagac cctaatggaa agcgcctcgc
     5941 tgacctatga acagcgtctg ttggcgattc aacaactggg gcgtgattac atggccgccg
     6001 ggttatatga ccgcgcggaa gacatgttca atcagctgac cgatgaaact gacttccgca
     6061 ttggcgcgct gcaacagttg ctacaaatct accaggctac cagtgagtgg cagaaagcaa
     6121 ttgatgttgc cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc gaaattgccc
     6181 atttctactg tgagttagcc ctgcagcata tggccagcga cgatctcgat cgtgccatga
     6241 ccttgctaaa aaaaggggcg gcggcagata aaaacagcgc ccgcgtatcc atcatgatgg
     6301 gacgcgtgtt tatggcgaaa ggagaatacg ccaaagccgt cgaaagtttg caacgcgtga
     6361 tatcccagga cagagaactg gtcagcgaaa cgctggaaat gctgcaaacc tgctatcagc
     6421 agttgggtaa aactgccgaa tgggcagaat tcctgcagcg cgcggtggaa gagaacaccg
     6481 gtgccgatgc tgaattgatg cttgctgata tcatcgaagc gcgcgacggt agtgaggccg
     6541 cacaggtcta tattacgcgc cagcttcagc gtcatccgac catgcgtgtg ttccataagt
     6601 taatggatta ccacttaaat gaagcggaag aagggcgtgc caaagagagc ctgatggtgc
     6661 tgcgtgacat ggttggcgag aaggtacgta gtaagcctcg ttatcgctgc cagaaatgtg
     6721 gttttaccgc atacaccctc tactggcatt gtccgtcttg tcgtgcctgg tcaactatta
     6781 aaccgattcg cggtcttgat ggcctgtaat tttttaaaaa aaccgacttt agttacaaca
     6841 tactagttat ttatgtccca ttgtgctccg gcaacggcgg cgcagaaaaa gcctgccagg
     6901 ggagaaatcg caactgttaa ttttttattt ccacgggtag aatgctcgcc gtttacctgt
     6961 ttcgcgccac ttccggtgcc catcatcaag aaggtctggt catgacgtta actgcttcat
     7021 cttcttcccg cgctgttacg aattctcctg tggttgttgc ccttgattat cataatcgtg
     7081 atgccgcgct ggcctttgtc gacaagatcg acccacgcga ttgtcgtctg aaggtcggca
     7141 aagagatgtt tacattgttt gggccacagt ttgtgcgcga acttcaacag cgtggttttg
     7201 atatctttct tgacctgaaa ttccacgata tccccaacac tgcagcgcac gctgtcgctg
     7261 ctgcagctga cttaggcgtg tggatggtga atgttcatgc ctctggtggg gcgcgtatga
     7321 tgaccgcagc gcgtgaggca ctggttccgt ttggcaaaga tgcaccgctt ttgattgctg
     7381 tgacagtgtt gaccagcatg gaagccagcg acctggccga tcttggcgtg acactgtcac
     7441 ctgcagatta tgcagaacgt ctggcagcac tgacgcaaaa atgtggcctt gatggtgtgg
     7501 tgtgttctgc tcaggaagct gtgcgcttta aacaggtatt cggtcaggag ttcaaactgg
     7561 ttacgccggg cattcgtccg caggggagtg aagctggtga ccagcgccgc attatgacgc
     7621 cagaacaggc gttggcggct ggtgttgatt atatggtgat tggtcgcccg gtaacgcaat
     7681 cggtagatcc agcgcagacg ctgaaagcga tcaacgcctc tttacagcgg agtgcatgat
     7741 gagtgattcc aacagccgtc tggtctactc aacagaaacc gggcgtattg atgagcccaa
     7801 agcggcccca gtacgctcta aaggcgatgg tgtggtgcgt attcagcgtc agaccagtgg
     7861 acgtaaaggt aagggcgttt gcctgattac cggtatcgat ctcgatgatg ccgaactgac
     7921 aaaacttgca gcggaactga agaaaaaatg cggctgcggc ggagcagtta aagatggagt
     7981 tattgaaatc cagggcgata agcgtgattt attaaaatca ctacttgaag cgaaagggat
     8041 gaaggtaaaa ctcgcaggcg gt
//
LOCUS       DBFIFS010000196         8057 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.196, whole genome shotgun sequence.
ACCESSION   DBFIFS010000196 DBFIFS010000000
VERSION     DBFIFS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.196"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..193)
                     /locus_tag="N7A07_003851"
     CDS             complement(<1..193)
                     /locus_tag="N7A07_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HGU2478432.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYR"
     gene            complement(455..1564)
                     /gene="apbC"
                     /locus_tag="N7A07_003852"
     CDS             complement(455..1564)
                     /gene="apbC"
                     /locus_tag="N7A07_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU2478433.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1696..3729
                     /gene="metG"
                     /locus_tag="N7A07_003853"
     CDS             1696..3729
                     /gene="metG"
                     /locus_tag="N7A07_003853"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU2478434.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3870..7679
                     /locus_tag="N7A07_003854"
                     /pseudo
     CDS             3870..7679
                     /locus_tag="N7A07_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7692..>8057
                     /locus_tag="N7A07_003855"
     CDS             7692..>8057
                     /locus_tag="N7A07_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGU2478435.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     TAAAEKAALKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSH"
BASE COUNT         2133 a         2035 c         1962 g         1927 t
ORIGIN      
        1 cacgatagtt ggtttcaact cgttgtaaca actgcccaca acgttgctga agatgattat
       61 taacaacatc catactgcat gtaggggaac taattctgcc attgatggtt aatgttgtag
      121 tatcagaaga gaacgcgggt gatgctagtc cataagcaag aaaaacagct cctgacaacc
      181 aatacttatt cattgaacgt tatccctgta gtaaaggtta tgccgggtag attgagtgca
      241 taacacacta tagctgtaca tccgctacac agccacgagg gatgataaag agacagtggt
      301 tgcacaatca atcgcctttg tacagtttcg gactaagatc cactgaatat tcgcagtatt
      361 aaataagtgt tcattaatgc ggcaaaaaca aatacaccgc atcaatataa catctctgcg
      421 gtgtatgaag aataaccaga atggttaata ggcgttagac cgcgcggaag gaaatctcgc
      481 ctggaatgac ttcaccctgc cagtagagct gagctgcaac gcggtcagcc agctggcggt
      541 agatagcggt aaattcgctc tccggacggc tgataaccgt tggcgtgcct ttatcgagat
      601 cctcacgtaa ggagatgtgt aacggcatct gccccagcag ctgtgtatgg tatttttcag
      661 ccagtttctc tgcaccaccg gtaccgaaaa tcggttcatg gtgaccacag ttactgcaaa
      721 tatgcacact catgttctcg acaataccga gcaccggaac ctcgactttc tcgaacatca
      781 caatgccttt tttcgcatcg atcaacgcga tgtcctgcgg ggtggtgacc acgactgcac
      841 cagttactgg aatgttttgc gccagcgtca attgaatgtc accggtgcct ggcggcatat
      901 caagcaccag atagtcgaga tccggccaca aggtttcctg caacatctgc attaacgcct
      961 tgctcgccat cggtccacgc cacaccatcg cattgtcgtc agtgaccaga taaccaattg
     1021 aattggttgc caggccatga gacataatcg gtgccatatg ggtaccgtcc ggcgaagttg
     1081 gacgctgatt ttctgcgccc agcatggtgg gaattgatgg gccgtagata tcggcgtcca
     1141 gaatgccgac tttcgcccct tccgccgcca gtgccagcgc caggtttacc gccgtggagg
     1201 atttacccac gccgccttta ccggaactga ccgcaatgat gttcttcacg ccattaatac
     1261 ccggctgatt cttcacgcgt tttaaggtgg cgatattgtg cgacagtttc cagtcgatag
     1321 ctttcgcccc ggtgatacgc agcagttcgg cgctacattg ctcttttaac tcttcaaacg
     1381 cgctatgcca gacgaatggc ataaccagtt ccacatgcaa cgtgtcgtcc atccaggcga
     1441 catggtgcaa cgctttcagc gtggtgaggt tatgcttcag ggttgggtgc tgaaaattgg
     1501 ccagtgtccc ggcgaccatt gcgcgcaagg cttctggtga tttggcctgg gattgttcgt
     1561 tcatcccgac tccttttttg tatagataaa ccatcagctg atagtttacc tgaagaatat
     1621 agagaagtac ttacttaaca ttttcccatt tggtactatc taaccccttt tcactattaa
     1681 gaagtaatgc ctactatgac tcaagtcgcg aagaaaattc tggtgacgtg cgcactgccg
     1741 tacgctaacg gctcaatcca cctcggccat atgctggagc acatccaggc tgatgtctgg
     1801 gtccgttacc agcgaatgcg cggccacgag gtcaacttca tctgcgccga cgatgcccac
     1861 ggtacaccga tcatgctgaa agctcagcag cttggtatca ccccggagca gatgattggc
     1921 gaaatgagtc aggagcatca gactgatttc gcaggcttta acatcagcta tgacaactat
     1981 cactcgacgc acagcgaaga gaaccgccag ttgtctgagc ttatctatac tcgcttgaaa
     2041 gaaaacggtt ttattaaaaa ccgcaccatc tctcagctgt acgatccgga aaaaggcatg
     2101 ttcctgccgg accgttttgt gaaaggcacc tgcccgaaat gtaaatcccc ggatcaatac
     2161 ggcgataact gcgaagtctg cggcgcgacc tacagcccga ctgaactgat cgagccgaaa
     2221 tcggtggttt ctggcgctac cccggtaatg cgtgattctg aacacttctt ctttgatctg
     2281 ccctctttca gcgaaatgtt gcaggcatgg acccgcagcg gtgcgttgca ggagcaggtg
     2341 gcaaataaaa tgcaggagtg gtttgaatcc ggtctgcaac agtgggatat ctcccgcgac
     2401 gcgccgtact tcggttttga aattccgaac gcgccgggta aatatttcta cgtctggctg
     2461 gatgcgccga ttggctatat gggttctttc aagaatctgt gcgacaagcg cggcgacagc
     2521 gtaagcttcg atgaatactg gaagaaagac tccaccgccg agctgtacca cttcatcggt
     2581 aaagatattg tttacttcca cagtctgttc tggccagcaa tgctggaagg cagcaacttc
     2641 cgcaagccga ccaacctgtt tgttcatggc tatgtgacgg tgaacggcgc gaagatgtct
     2701 aagtctcgcg gtacctttat taaagccagc acctggctga atcattttga cgccgacagc
     2761 ctgcgttact actacactgc gaaactctct tcgcgcattg atgatatcga tctcaacctg
     2821 gaagatttcg ttcagcgcgt gaatgccgat atcgttaaca aagtggttaa cctggcctct
     2881 cgtaatgcgg gctttatcaa caagcgtttt gacggcgtgc tggcaagcga actggctgac
     2941 ccgcagttgt acaaaacctt cactgatgcc gctgaagtga ttggtgaagc gtgggaaagc
     3001 cgcgaatttg gtaaagcagt gcgcgaaatc atggcgctgg ctgatctggc taaccgctat
     3061 gtcgatgaac aggctccgtg ggtggtggca aaacaggaag gccgcgatgc tgacctgcag
     3121 gcgatttgct caatgggcat caacctgttc cgcgtgctga tgacttacct gaagccggta
     3181 ctgccgaaac tgaccgagcg cgcagaagca tttctcaata cagaactgac ctgggatggt
     3241 atccagcaac cgctgctggg ccacaaagtg aatccgttca aggcgctgta taaccgcatc
     3301 gatatgaagc aggttgaagc actggtggaa gcgtctaaag aagaagtgaa agcagctgcc
     3361 gcgccagtta ctggcccact ggcagatgat ccgattcagg aaaccatcac ctttgacgac
     3421 ttcgccaaag ttgacctgcg cgtggcgctg attgaaaacg cagagtttgt tgaaggttct
     3481 gacaaactgc tacgcctgac gctggatctc ggcggtgaaa aacgcaatgt cttctccggc
     3541 attcgttctg cttacccaga tccgcaggca ctgattggtc gtcacaccat tatggtggct
     3601 aacctggcac cacgtaaaat gcgcttcggt atctctgaag gcatggtgat ggctgccggt
     3661 cctggcggga aagatatttt cctgctaagc ccggatgccg gtgctaaacc gggtcatcag
     3721 gtgaaataat cccccttcaa ggcgctgcat cgacagcgcc ttttctttat aaattcctaa
     3781 agttgttttc ttacgatttt gtctctctct aacccgtata aatactggta gcatctgcat
     3841 tcaactggat aaaattacag ggatgcagaa tgagacactt tatctatcag gacgaaaaat
     3901 cacataaatt ctgggcggtt gagcagcagg gaaacgagct gcatatcaac tggggcaaag
     3961 tcggcactaa cgggcaaagc cagataaaaa gttttgcgga tgttgcagca gcggaaaaag
     4021 cgaaactgaa gcttatcgcg gagaaaacga aaaaaggcta tgtggaaaat gcttcagcaa
     4081 acgtgcatat cccccccatc accaaagcca ctcctgaagt tgaaacttcc cctgagagta
     4141 aaaaccaacg cccgtggctg gctgatgatg cagttattcc ggtaactgac gatattaacc
     4201 gatttgcttt tcctcatcgc tctcgcccca gagagatcaa ttatctgcgc aaagacggtg
     4261 agatatggaa gcgtattgca gataacacca gggcatatga tcccgacaac aattaccgct
     4321 cttatcctga aaactggcag caggcttttg ctgagttaca aaggcgaatt ctggataatc
     4381 aacagacagg gagtgcgcaa tctgatgcag cattgctctg gagtttttgg aactcttact
     4441 ctgcagatga actggtcgat gacttagtca ttcgctgtgg tctggaaagc gccgttgaaa
     4501 tcgtcctttt tgcccttcaa ctcaaatata aaccagtaaa aggcgcagta accaccatca
     4561 ttccacctaa tcacgaagca gaatcgctac ctagttagca tcaacgtcta tgtcatcatc
     4621 tttcactcgc ttcagaagat gagtggcaac gctgtgtaga taaagtactc accgccattc
     4681 cgacgctatc ccccgcacgg caaccttttg cggcgttact gctccctgaa cgcccggata
     4741 tcgcccatgc tatagatcta cgctatgcag accaaaacgt cccggcgatg acctggttaa
     4801 gactggtgat aaccgatgat actgctctga gtacagtgga aaaatacgac ttcccaccgt
     4861 tgtatcgtga ttttcgtaat tttcgcgctt atctggcaat gttgttggca aataatgggg
     4921 tgcgcggtgt aagccggata cttcttgagt ttacggaaga ccattctgac aatcccactt
     4981 atctcttcga acgcatcagc gagactgaaa atcttgtcaa atggctatgg aaaacgaatc
     5041 acccggatgc gattcaaatt ctgatcctcg gtgtaattgg taagaaaaag cacctggaat
     5101 acttaagcaa ggccagtcaa aagcatcccg ctgcggctat tgcggcttat gctactttgc
     5161 tggcaataca tgaagataaa gagtggcgta aagcgcttgt caaactgatt accgccacgc
     5221 cagagttagt atgcgacgtc attccctggg ttaatgctaa agcggcaggc atcttatctg
     5281 aatgtcgtcc gcagtcagtc gctgaagaat gtgaatatgc cactgtggat atgttgccgg
     5341 agctgcttgt ttcgccgccc tggatgacca aagagaaaaa gaaaaatacg cctgtgtttg
     5401 atcttccagt gcttccagtt ccttctgttt ctgatgtcac tccagagatt accaaaaaac
     5461 tgacccgtac ttaccttgtc acccacttcc agcaaatagc ccaacagcag gcgacaaaac
     5521 aaacattgtt taccgacctg ccgccgatca aaaaagcaag ctgggagaaa cacttaatac
     5581 cgcttacacc ggagcaacaa atactgtggc atcttggttt tgaaaaatgg cgggagagcg
     5641 gagaaaaaat atacgaaaaa attccggcgc cacaaagcgc cgttgatgcc ctgctacgct
     5701 tcgattttcc agcactcaat gctgaatttg tgcattatca taacaatgca tacaaaagct
     5761 ggaatctcat tgccctctgt tacctgcctg gtcaacaggc catttctttt cttaaccaga
     5821 tagtcaaaga agataactac tctggtgagg gaaatattct ggcaattttt ggtagcgccg
     5881 ccatacctgc atttatggcc tgcctgcaac gtgatccacg acgtctgtgc ttcttcccct
     5941 ttttcctcgg tgtcagtgaa ctggccttac cgatggcgca acaattacag aaaaaaatgt
     6001 cttatgaaga tgcgcgaaac tggctaactg attatccacg ccatgccgcc gccgggctgc
     6061 ttcctgtggc cctgggcaaa aaaggaaaag atcgtgattg tgcccgccag gcgcttcgtc
     6121 tgctggtgaa tttaaaccag cgagaaacga ttgaggaaat cgcgcaagga tataatcaac
     6181 ctgatgtttt agctgcgctg gcaacattgt tcgatagcga cccactggaa gaatatcccg
     6241 ctaaaatcgc cccactgccc ggcttttatc agttcacctt gtggcgcaga ccacggctta
     6301 aaagtaacaa cctgcctctg tcagatgacg ctatgcgcca cctcggcact atgctgagct
     6361 ttcctcgcga cattaccgcc tacgctgggc tggatatcat tagagaaatc ttcacccgcg
     6421 aatcactggc tgaatttggc tgggatctgt atcccgcctg gacagaagct ggcgcacccg
     6481 caaaagaaaa ctgggcattt acttcgcttg ggattttagg caatgacgac accgcgcgta
     6541 aattaacccc gcttatccgc gcctggcctg gggaatccca gcataaacgg gcggtgtccg
     6601 ggctggatgt attagctgat attggcagcg atgttgcgct aatgctgctt aatggcatcg
     6661 cgcgaaaaat taaattcaaa gcattacagg aacatgcccg cgagaaaatc aacatagttg
     6721 ctgaaaaccg tgggctgact atggctgaac tggaagaccg cctggctcca gatttagggc
     6781 ttgatagcag tggctcgctg atactggatt tcggcccccg caaattcacc gttggttttg
     6841 acgaaacctt aaaacctgta gtgtgcgatg caaacggcaa agtcctgaaa gatttaccta
     6901 agccaaacca gagcgatgaa aaaactcagg caactgacgc ggttaatctc ttcaaacagt
     6961 tgaaaaaaga tgtacgcgcc atagccagcc agcagattga tcgtctggaa caggctatgt
     7021 gccagcgcag acgctggacg gcagagcagt tccgcctgtt tctggtggag catccgctgg
     7081 tacgtcactt aacccgacgg ctgttatggg gggtttataa cgatgaaaac gcccttatca
     7141 cctgttttcg cgtggcagaa gacagcactt acagcgatgc gcaggatgag ttattcacgc
     7201 tgccagcagg aaacatcggt attccgcatg tgctggaaat atcccctgaa tccgccgctg
     7261 cattcaggca aatttacgct gattacgaac tgcttccccc tttccaacag ctcgagcgag
     7321 gtagctatca ccttgctgat aatgaacgta atactcacga actgacacgc tggcaaggac
     7381 ggctttgcca ggccggacgc attgtcgggc tggaacgcag aggctggcaa cgtctggaag
     7441 aaagcggcag cgtttatgcg atgcgcaaaa caacccctca tggcgatctc gaacttgaaa
     7501 cggaaccttt ttcattaatt tatggtgaaa cggggtatgg cgaccagcac ccggttgaaa
     7561 gcgtaaaaat cacctcgcca gatgatcgct acggtaaaca atcctcactc actttctcca
     7621 tgctcgacga catcaccgcc agcgagctga ttaacgacat tgaatcactg tttgattaaa
     7681 aggatttaac aatgaaaacc tatatttatc aggacgaaaa atcacataaa ttctgggcgg
     7741 tggagcagca gggaaacgag ctgcatatta actggggtaa agtcggtacc aacgggcaaa
     7801 gtcaggtaaa aagctttgca gatactgcgg cggcggaaaa agcagcactg aagctgattg
     7861 cggagaaggt gaagaagggg tatgtggagc aagtagacgc taacgctcac tcacatgatg
     7921 cctcagcaaa gaaagtcctg gtcacagagg aaaaagcctc aataaatcgc caggcagcaa
     7981 ctaatggttt accctggctg gcagacaacg atcccattat tcttcccccg aatattgccc
     8041 gccatgccct cagccac
//
LOCUS       DBFIFS010000197         8016 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.197, whole genome shotgun sequence.
ACCESSION   DBFIFS010000197 DBFIFS010000000
VERSION     DBFIFS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.197"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            5..211
                     /locus_tag="N7A07_003856"
     CDS             5..211
                     /locus_tag="N7A07_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2478436.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            237..689
                     /locus_tag="N7A07_003857"
     CDS             237..689
                     /locus_tag="N7A07_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2478437.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            746..979
                     /locus_tag="N7A07_003858"
     CDS             746..979
                     /locus_tag="N7A07_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU2478438.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            1045..3003
                     /locus_tag="N7A07_003859"
     CDS             1045..3003
                     /locus_tag="N7A07_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU2478439.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            3058..3492
                     /gene="psiB"
                     /locus_tag="N7A07_003860"
     CDS             3058..3492
                     /gene="psiB"
                     /locus_tag="N7A07_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU2478440.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            3489..4250
                     /locus_tag="N7A07_003861"
     CDS             3489..4250
                     /locus_tag="N7A07_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU2478441.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            4424..4573
                     /locus_tag="N7A07_003862"
                     /pseudo
     CDS             4424..4573
                     /locus_tag="N7A07_003862"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4515..4640
                     /locus_tag="N7A07_003863"
     CDS             4515..4640
                     /locus_tag="N7A07_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGU2478442.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            4774..6795
                     /locus_tag="N7A07_003864"
                     /pseudo
     CDS             4774..6795
                     /locus_tag="N7A07_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6863..7294
                     /locus_tag="N7A07_003865"
     CDS             6863..7294
                     /locus_tag="N7A07_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HGU2478443.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
BASE COUNT         1959 a         1904 c         2422 g         1731 t
ORIGIN      
        1 tgaaatgcac atcccgcaaa actttttgcc caaaagcagg caaaaagttt tgcggtctgc
       61 ccccggacag tcgtttccgg ccgtggcagt gatgcccctt gcggcgaagg ttctcaaaaa
      121 ggtgatggtg aataccggag ggaacaaaag gcaggtcccc gcaaaacgtc aaaaccgcgg
      181 ctcccgaaac tcaaaagatg atgggggctg aaaacatcaa cagaaggaga cacatcatgg
      241 cagttcgtgg cattaacaag gtcatcctcg taggacgcct gggaaaagat ccggaagtcc
      301 gttacatccc caacgggggc gcagtggcaa acctgcaggt ggccacgtca gaaagctggc
      361 gtgacaaaca gacgggggag atgcgggagc agacagaatg gcatcgcgtg gtgctgttcg
      421 gcaagctcgc ggaagtggca ggtgaatatc tgcgcaaggg cgcgcaggtc tacatcgaag
      481 gtcagcttcg cacccgtagc tgggaggata acggtatcac ccgttacgtc accgaaattc
      541 ttgttaagac cacgggcacc atgcagatgc tggtacgtgc cgcaggtgct cagactcagc
      601 cggaagaggg gcaacagttc agcggtcagc ctcagccgca accgccggag ggtgacgatt
      661 acgggttttc agacgatatc ccgttctgaa cgactgactg tgacaaccgc cccgtcctgt
      721 gcggggcatc accggagaga tgaggatgag cgaatatttc agaatacttc agggactgcc
      781 tgacggtcct tttacccgca aacatgccga agccgttgcc gcacagtacc ggaacgtctt
      841 tatcgagaat gaccacggcg aacagtttcg cctggttgtc cgtaataacg gtgcaatggt
      901 ctggcgtacc tggaattttg aggacggtgc cgggtactgg atgaaccatg tcatccgtga
      961 tttcgggatt attaagtaag agaggtgccg gacgggaacg cgtccggcag acaatcatta
     1021 ttaccgtttt gggaggtact aactatgtca gttacagagt ctaaggcaaa aacggagcgt
     1081 aaatccagcc gtaaacctgc aaaaacgcag gaaacagtcc tgtcggccct gctggcgcag
     1141 acggaggaag tgagcgtgcc gctggcctcg ctgattaagt caccgctgaa tgtgcgcacg
     1201 gtgccgtatt ctgcggagtc cgtcagcgaa ctggcggact ccattaaagg tgtcggcctg
     1261 ttacagaatc tggttgttca tgccctgcca ggtgaccgtt acggtgtcgc cgcaggtggt
     1321 cgccgactgg cagcactcaa catgctggca gagcgtgaca tcattccggc tgactggcct
     1381 gtccgtgtga aggtcattcc gcaggagctg gcgactgccg catcgatgac cgagaacggt
     1441 catcgtcggg atatgcaccc tgccgaacag attgccggat tccgtgcaat ggcgcaggaa
     1501 ggcaaaacac ctgcacaaat cggtgatttg ctgggttatt cgccccgcca cgttcagcga
     1561 atgctgaaac tggcagacct tgcgcctgtc atcctcgatg cgctggcaga agaccgcatc
     1621 accaccgagc actgtcaggc gctggcgctg gagaacgaca ccgcgcgtca ggtgcaggtg
     1681 tttgaagccg cctgtcagtc gggatggggc ggtaaaccgg aagtacagac cattcgtcgt
     1741 ctggtgaccg aaagtgaagt ggcggtggca gggaacagta aattccgctt cgtgggggct
     1801 gataccttct cgccagatga actgcgcacc gatttgttca gtgacgacga gggtggctat
     1861 gtggactgcg tggcgctcga tgccgccctg ctggaaaaac tccaggctgt cgccgaacac
     1921 cttcgggaag ccgaaggctg ggaatggtgc gccggacgta tggagcctgt tggtttctgc
     1981 cgtgaggatg ccggaacata ccgcagtctg ccggagccgg aagcggtgct gacggaggca
     2041 gaagaagaac gcctgaacga actgatggca cgttacgatg cgctggaaaa ccagtgtgag
     2101 gaatccgacc tgctggaagc cgaaatgaag ctgatgcgct gcatggcgaa ggtcagggcg
     2161 tggacgccgg agatgcgtgc cggaagtggc gtggtggtgt cctggcgtta tggcaacgtg
     2221 tgtgtccagc gtggtgtgca gttgcgcagt gaggatgatg tggctgataa cgattaccgc
     2281 acggaacagg tgcaggagaa agcctcagtg gaggaaatca gtctgccgct gctgacgaaa
     2341 atgtcctcag agcgcacgct ggcagtccag gcggcactga tgcagcaatc agacaaatcc
     2401 ctggcacttc tggcatggac actctgtctg aatgtatttg gcagtggagc gtacagcaat
     2461 cccgccagaa tccgcctgga atgtgagcat tattcgctga ccagcgatgc gccgtcaggg
     2521 aaggaaggtg ccgcattcat ggcgatgatg gcagaaaaag cccgtcttgc cgccctgctg
     2581 ccggatggat gggcacggga tatgacgacg ttcctgtcac tcagtcagga ggtgctgtta
     2641 tcactgctca gtttctgcac cgcgtgcagt atccacggtg tccagacccg tgagtatggt
     2701 cacacgtcac gcagtccgct ggacacgctg gagagcgcca tcggctttca catgcgcgac
     2761 tggtggcagc cgacgaaagc aaactttttc ggacacctga aaaagccgca gattatcgca
     2821 gccctgaatg aggcaggact atccggtgcc gcacgggacg cggagaagat gaagaaaggc
     2881 gatgcggctg aacatgcaga gcaccatatg aaagacaacc gctgggtgcc tggctggatg
     2941 tgtgcaccac gcccacagac ggatgccact gaacgcaccg ataacctggc tgatgccgcc
     3001 tgatgaacaa ccacaccgcc ccgccggaga cggggcggca gcaagggaga taccgtgatg
     3061 aaaactgaac tgactctgaa tgtattacat accatgaacg cacaggaata tgaagatatc
     3121 cgggctgccg gaagtgatgc acgccgtgag ctgacacacg ccgtgatgcg ggagctggat
     3181 gcgccggaca actggacgat gaacggcgag tacggcagcg agtttggcgg ctttttcccc
     3241 gtccaggtcc gtttcacgcc tgcccacgaa cgttttcacc tggcgttatg ttcaccgggc
     3301 gatgtctccc aggtctgggt gctggttctg gtgaatgccg gtggtgagcc gtttgcggtg
     3361 gttcaggtac agcggcggtt tgcaccggaa gccgtcagcc attcactggc actggcggcg
     3421 tcactggata cgcagggata cagcgttaac gacatcatcc atatcctgat ggcggaagga
     3481 ggtcaggtat gagcgcacgt tcacaggcac tggttcccct cagcacagag caacaggccg
     3541 catggcgggc agtggcagag acggaaaaac gccgcaatca gggaaacacg ctggcggaat
     3601 atccgtatgc gggggcgttt ttccgctgtc tgaacggaag ccgcaggata tcgctgtctg
     3661 acctgcgatt tttcatgccg tccctgaccg cagaagaact gcacggaaac cgtcttcagt
     3721 ggttgtatgc catcgatgtg ctgattgaaa cacagggcga ggtctgcctc cttccccttc
     3781 ccggtgatgc agcagaacgc ttgttcccgt cggtgcggtt ttgtgtcagg gaacgcagtc
     3841 gccataaatc cgcgctggtc atgcagaagt acagccgcca gcaggcgagg gaggcagagc
     3901 agaaagcccg tgcgtaccag gcgctggtcg cccaggctga aatcgaactg gcgttccatt
     3961 caccggaaac agtcggaagc tggtatgcac gctggagtga tcgggtggca gagcatgacc
     4021 tggaaaccct gttctggcag tggggcgagc gttttccgtc actggccgga atggaacgct
     4081 ggcagtggca ggatatgcca ttctggcagg ttatcgcgga agccagtctg gcagcaaagg
     4141 aggccggtca tgctgtccgt gagatggagc ggtggatggt gccgaacaaa ctccgggagg
     4201 tgcgtgatgc ggcaacaatc acacggattg cccgtaaaca gcctgaatga gcgggttatt
     4261 ttcaggaaaa gtgaatgtgg tcagcgtgct ggtatatggg ctatgatgtg cccggcgctt
     4321 gaggctttct gcctcatgac gtgaaggtgg tttgttaccg tgttgtgtgg cagaaggaca
     4381 aaagccccgt agttaatttt tcattaaccc acgaggcccc tgcatgctta gacaacatca
     4441 ggatagcctc ttaccgcgct ttgcgcaagg agaagaaggc catgaaacta ccacgcagct
     4501 ctcttgtctg gtgtgtgttg atagtgtgtc tcacactgtt gatattcact tatctgacac
     4561 gaaaatcgct gtgcgagatt cgttacagag acggatacag ggaggtggcg gctttcatgg
     4621 cttacgaatc cggtaagtag caacctggag gcgggcgcag gcccgccttt tcagggctga
     4681 tgctggtctg actgcactga agcgccttta taaaggggct gctggttcgc cggtggcccc
     4741 tttctccttg ctggcattgt acgggcatga acagcctggc gacgcgggaa aaagcagacg
     4801 ttcagggaat atattgctca gatgtcgatt gccgggctgc gtgaagagcc cgcacaggac
     4861 tggttatctt tgcataaacg gctggccagt gatggtctgt atatcacgat gcaggaaggg
     4921 gaactggtgg tgaaggatgg ctgggacaga gcgcgggaag gtgttgcact cagttcgttc
     4981 ggaccgttat ggacagctga aaaactgggg cggaaactgg gggaatatca gcctgtaccg
     5041 acagatattt tcagtcaggt ggggacaccc ggtcgttatg atccggaagc cataaacgtt
     5101 gatatccggc cggaaaaagt ggcagaaacg gagagtctga aacagtacgc ctgccgtcat
     5161 tttgccgaac gcttaccgga aatggcgaga aatggtgagc tggaaagttg cctggatgtg
     5221 cacagaactc tggcaacggc tggattatgg atggggatcc agcacgggca tctggtactg
     5281 catgatggat ttgataaaca acagacacca gtacgtgcag acagtgtgtg gccgttaatg
     5341 acgctggatt atatgcagga tctggatggt ggatggcagc ctgtaccgaa ggatattttt
     5401 actcaggtta tacccggaga acggttccgg ggacgtaatc ttggtactca ggctgtcagt
     5461 gattacgaat ggtatcgtat gcggatgggg acaggtccac agggagcaat aaagcgcgaa
     5521 ctgttttctg ataaggaaag tctgtggggg tacacaactg ttcagtgtga gtcgttgatt
     5581 gaagatatga tcgcgggcgg gaatttcagc tggcaggcgt gccatgagat gtttgcgcgt
     5641 aaagggctta tgttgcagaa acagcatcat ggtcttgtga ttgtggatgc gtttaatcat
     5701 gagctcacac cagtgaaggc cagcagtatc cacccggatt tgacgttatc cagggctgag
     5761 ccgcaggcag ggccatttga gattgcaggt gcagatatat ttgaacgggt gaagcctgaa
     5821 tgtcgctaca atccggaact tgctgccagc gatgaagtgg agcccggctt ccggcgcgat
     5881 cctgtactgc gtcgtgaacg ccgtgaagcc cgtgcagctg cgcgggaaga tttgcgcgcg
     5941 cgttatcttg catggaagga gcactggcgt aaacccgact tacgttacgg agaacggtta
     6001 cgggaaattc atgctgcctg ccgtcggcgc aaggcgtata tccgtgtgca gttccgggat
     6061 ccacaattgc gtaagctgca ttaccatatt gcggaagtgc agcgtatgca ggcgctgatc
     6121 aggctgaagg agagtgtgaa ggaagagcgg ttgtcactga ttgaggaggg taagtggtat
     6181 cctttgtcct accggcagtg ggtggagcaa caggcggtac agggagacag agctgcatta
     6241 tcacagttac gtggctggga ttatcgtgat cgtcgcaaag acaagcgacg aacaacgaat
     6301 gccgaccgct gcgtgattct ctgtgaaccg ggaggaactc cactttatga agacactggc
     6361 gttctcgaag cccgtctgca gaaagatggc agtgtgcgtt ttcgtgatcg gaggaatggc
     6421 gagttagtct gtgtggatta tggggaccgt gtggtgttct accatcatca ggacagaaat
     6481 gaactggtgg ataagctgaa tctgattgct cctgtattgt ttgatcgtga gccaggaatg
     6541 ggttttgaac cggaaggctc atatcaacag tttaatgatg tatttgcgga aatggtggcc
     6601 tggcataatg cagctggaat aaccggaaat ggacactttg taatcagccg cccggatgtg
     6661 gatttacatc gtcagcgtag tgaacagtac taccacgaat atatcaggca acagaaaagc
     6721 atatccgggg ggcatggcgc atcttatgct ccagttcagg ataatgagtg gacgccacca
     6781 tcacctggaa tgtagggatg aggagtggat attaaaaatc ttaacttgta ttagttaaga
     6841 ttttctttgc tctgacagag ctatggtact gagatctata tgggttgagg agcaatatat
     6901 gggggtaaag aaaatcgtta tactggctat tttagtcagc tttgtggcgg gatgtgcgcc
     6961 attacatcct tcaaattgcc ataaaactac agccctgggt agttgcagtt caggacgttg
     7021 ggatgatcag gatgaatggg gggagcaggc acggggaatc agggatgcaa ttaatgctaa
     7081 acttgatgat ccgcaaaaat ggaaaaggaa aaaatgcagg ttgcatatgg aattctcccg
     7141 ggatggcacg gcgttaaaaa tatcaaccag taatggcgat aaagcctatt gcgaagcgat
     7201 aaagtcagca gcccataaag ctaaattccc ggccttcaac aatccggaag tttatagaga
     7261 ttttcaaaaa tccggctttg acatgcgcgg ttagctacac gacatatctg tagggctaac
     7321 agaagactgt caggtcggct gattactgga acctgaggta ctttgcctca ggttatgttg
     7381 acatactcaa aaaaagattc ttacattatc ccggtaacgg cgtccttcat cggcctgtcc
     7441 gttaaactca ggctacctca cacctcacac ctcacacctc acacctcaca cctcacacct
     7501 cacacctcac acctcacaac agcgggtctg gacagaaagc ggctcttgat gtggattatc
     7561 gcatggtgtg ggctttcagc ggcatggtga ctggaaagta acgatattgc atctgtggca
     7621 acgaaaattg cagagttggt actctttgaa ctgcataagt aagcagaaag gtaaggttat
     7681 gctttgcgat tgaaattcag ccttaacgta caataatttt cgatatccaa actgcccccc
     7741 tataacagca aaaggtccca caagacgctc gtggaatata ttttaaaaga gaacaaaaat
     7801 tgcaatatgt ggtggctcca tacagaggat tgacaacatt cccatgctaa ttgttaagat
     7861 tcaaattgaa gtatatctat ctgtcatttt tgcaaatgac atgcggtagc ataacgttcc
     7921 gggggctcct gtgccgtcat gcgtgacgac attgatcagc caataatggc acgtgaccac
     7981 agccagatac ggccagcctt catccgtacg taaata
//
LOCUS       DBFIFS010000198         7986 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.198, whole genome shotgun sequence.
ACCESSION   DBFIFS010000198 DBFIFS010000000
VERSION     DBFIFS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.198"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1217
                     /gene="yjeM"
                     /locus_tag="N7A07_003866"
     CDS             <1..1217
                     /gene="yjeM"
                     /locus_tag="N7A07_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU2478444.1"
                     /translation="IWMVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVA
                     WMILVTVVASKGINKIARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLAS
                     PNPGYQSGLAMLSFVVFAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYS
                     LAIFLWGVSTNWQQVLSNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFAR
                     ITGLSMFLAYTGAFFTLCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLV
                     TVFILLVSFGGGTASAFFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKT
                     RMSAMIATVVVVLVVTFANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYC
                     SRMANKPELALD"
     gene            complement(1368..4691)
                     /gene="mscM"
                     /locus_tag="N7A07_003867"
     CDS             complement(1368..4691)
                     /gene="mscM"
                     /locus_tag="N7A07_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU2478445.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4713..5681)
                     /gene="asd"
                     /locus_tag="N7A07_003868"
     CDS             complement(4713..5681)
                     /gene="asd"
                     /locus_tag="N7A07_003868"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU2478446.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5778..6830)
                     /gene="rsgA"
                     /locus_tag="N7A07_003869"
     CDS             complement(5778..6830)
                     /gene="rsgA"
                     /locus_tag="N7A07_003869"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU2478447.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6925..7470
                     /gene="orn"
                     /locus_tag="N7A07_003870"
     CDS             6925..7470
                     /gene="orn"
                     /locus_tag="N7A07_003870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU2478448.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7681..7756
                     /locus_tag="N7A07_003871"
     tRNA            7681..7756
                     /locus_tag="N7A07_003871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7714..7716,aa:Gly,seq:gcc)
     gene            7792..7867
                     /locus_tag="N7A07_003872"
     tRNA            7792..7867
                     /locus_tag="N7A07_003872"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7825..7827,aa:Gly,seq:gcc)
BASE COUNT         1813 a         2098 c         2150 g         1925 t
ORIGIN      
        1 tcatctggat ggtgagcacc tccgcgaaag tttgggtacc gttctcaaca ttcctctatg
       61 gtagcgacat gacccagcac tggcgtattg ccggactgga gcctacgcag gtggttggtc
      121 tgctggcagt ggcatggatg attctggtca ccgtcgttgc ttcaaagggg attaataaaa
      181 ttgcccgcat tactgcggtg ggcggtattg cagtaatgtg tctgaattta gtattgctgt
      241 tagtaagcat tactattttg ttattaaatg gtgggcattt cgcgcaggat attaatttcc
      301 ttgcatcacc gaacccaggt tatcagtccg gtctggcaat gctatcgttt gtggtatttg
      361 ctatttttgc ctatggcgga attgaagcgg ttggtggtct ggtcgataaa acggaaaatc
      421 cagaaaagaa ctttgccaaa ggtattgttt ttgccgctat tgttatttca atcggttatt
      481 cgctggcaat atttttatgg ggcgtcagca caaactggca gcaggtatta agtaatggtt
      541 ccgttaacct cggcaatatt acctatgtgc tgatgaagag cctcggggtg acgctgggta
      601 acgcactgca tttgtcacct gaagcgtcat tgtcgctggg cgtatggttt gcgcgtatta
      661 ccggactttc gatgttcctc gcttataccg gtgcgttctt tacgctttgc tattcaccgc
      721 tgaaagccat catccagggg acgccgaaag cgttgtggcc ggaaccgatg acgcgcctga
      781 atgcgatggg gatgccgtct atcgctatgt ggatgcagtg cgggttggtt actgtcttca
      841 tcctgctggt ttcgtttggt ggcggtaccg catcggcgtt ctttaacaag ctgacgctga
      901 tggcgaacgt gtctatgacg cttccttacc tgttcctcgc gctggctttc ccattcttta
      961 aagcacgtca ggatctcgac agaccatttg tgattttcaa aacgcgtatg tcggcaatga
     1021 ttgcgacggt ggttgtcgtt ctggtggtga catttgcgaa cgtcttcacc attattcagc
     1081 ctgtggttga agccggagac tgggacagca cattgtggat gattggcggc cctgtcttct
     1141 tctcgctgtt agcgatggcg atttaccaga actattgcag tcgcatggcg aataaacctg
     1201 agttagctct cgactgatca tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt
     1261 gtcaggaagt aatgcgtatg gatgatactc tccagggccg gtgataactg aagatgatgt
     1321 taagctgcta aatgagccac gttgacatca tttggcagct tcatgcatta caaacttccc
     1381 gcctgacgac ctttgcccgc agacgtcagc gttctccccg tttgtttacc gttgaggctt
     1441 tccagacgca tctggaaggg cgggaatggc atatcgatac cgtgggcatg gaagccagcc
     1501 agaatcagct ggtggatctc atggcgtagc ggcatacggt gacccatctc agcggcgtaa
     1561 atacgcagct cgaaaatctg aatcccctgt tgcagatcca ccaggaagac ttccggtgcc
     1621 gggttgtcga tcaccagcga gcagcgacgc gctgcggtga gcaggatttc cgtcacttct
     1681 tcactgttgg catcggcagg ggccggaatc gtcaacacca cgcgcgtgac cgagtcagag
     1741 agcgaccagt tgataaactg ctcggtaata aacgccttgt tcggcacgat tatctcttta
     1801 cggtcccagt cgctgatggt ggttgcgcgg gtattaattt tcgtcacgct accagtaaga
     1861 tcgcgaattg tcaccgtatc gccaatgcga atcggttttt cgaacaggat gatcaggcca
     1921 gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggcg
     1981 accagccact gcaatttcga ccactcaata ccaatcatcg agaagccgac cagcccgcca
     2041 atcagcatca gcagatattt ggtgatggtg gtgatggcgt agccggtacc cggcgttaaa
     2101 tccaggtgct gcaaaatcgc cagttccagc agcgcgggca gattgcgcac cagctgcgtg
     2161 gtgatgataa acaccagaat ggcaatcagc accgcaccga gggtaatcgg ctccagactt
     2221 tctacgccct gtaccgtgga ggtgacatcc cacagcgaaa tattttcgag gaagccgaaa
     2281 gcggaatgga tttctgacca cagcacaatg accgaaagca gggcgatcaa catcaaaatt
     2341 gagcgcacta gccgcaagga ctgcgcactg atggcatcga gatcgacttc gctttcatcg
     2401 acttcaattg ccccttccgg gctactgtga tgatgtgctt cctcttcgcc acgcgcacgt
     2461 tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa acgccagcct gcgtcgttgg
     2521 atcagcatcc agcggcggat aacgtgataa accaccagta gcaggaacca gatggcaacc
     2581 gaggtttcaa gcctcgctaa cagcgcctgt gccgttgcca gataacccac cgccgacgcc
     2641 agaatggcaa ccagtggcgc gccaatcatc atgttccaca acatatggtt ggtaatattg
     2701 tcgccgctgc cttctttgtt gagatacagc gggatcccgg cctttttcag gctgagggtg
     2761 accaccgcca gcgcaccgca aatgaggata aagcaaagcc gtcccagcga accggaaaat
     2821 tcgcggtcgt cgaggttatc gaacatcatc agcgccataa tcagtggcac aataagcccg
     2881 atgctcatca ggtagtagcg catcccacga gaaacacgtt cgcgcggcca gccaaaatga
     2941 gcgataaaca agccgttcgg gcgggcaaag gtagcgcaaa tcatcactac ccacagcagc
     3001 ggcacggtgg ctgtgacgcc atcaccaatc gcgaccgcca gcggataagg ccacgcctcg
     3061 cgcaagccgt aacccagcgt catccacagc accggcagcg gtgacgcgac gagaatcgac
     3121 cagaaaagag tgcgcaacgt cagccagaag tgatcctgag tcactttgcc gactttcgcc
     3181 gccgaacgtt caagaaaacg ggtgaaatag cggcgcgagt aaatactgca accgaccaga
     3241 atcaacgcgc caaacagcgg caaaatcgtc tctttgctgg tcagcatcat cacactggct
     3301 ttgcccaact gactgaaggt gtccagcgaa atgagacgac gcagatcctg ggcgatttcc
     3361 agcggccagg cgatggtcat cgggcgcacg tcagaggtcc agaacagata gcggtgggtc
     3421 gcttcgttca cttctttcag cgcatcttcc agttgcccgt tggagacttt cagcttggtc
     3481 agttccagca gtagcgtatc gccaccctgc aacaatgagt tcagcaactc acgctgagtg
     3541 cgtagctgtg cttccagaat acggttttgc tcagcggtca gcggctgacc gtcggcctga
     3601 tgaatttgcc gtagcagcgg ctgtttatta agcagatcct cataacgtaa ccgttgcaca
     3661 cgcaactgcg ccatttcggt atcaagctgt tgtggtttcg gcatttccgg cagccgtgcc
     3721 acctgcgccc gcaacgcttc accgagcaga ttggacgatc ccagccattg cgactgttca
     3781 cgcagcgtat tcaacgcctg ccggacctgt aacgtctggc tggcagcctg acgctgttgc
     3841 gaggcaacga gatccatccg ctgcgcctgt tgattcagcg ccgccgatag ttcgcggtta
     3901 attttgaatt gcgcgacgat atctttcggc aaatcggcac tgttttctgc tagtaattcg
     3961 gtactttcca gcgcccgctc tgcctcaagc tggcgttggc tgttcaattg attacgcaag
     4021 gcctgcaaat acgcatccag ttgctggctc tctttttcag ccagctcaga gcgcaagcgc
     4081 gctaattcct ggcggttatt ggcagacagc tgcgccagct ccagttcatc aacgagcgcc
     4141 ttaagacgtg cagagtcaga ctgcaacgcg aaattttgtg cctgattgag cggagtattg
     4201 ccggtaagcg tccccaggcg gcgctcgatc tcatttaact gacggcgggc gtcggtttgc
     4261 tgttgcggca gttgattcag cgaatcggca atctcgcggg cgcgctcctg ctcttgctgg
     4321 gcctgacggc ttttatccag caactgactg ctgacctgga gaatttcctg attcagcgcg
     4381 tcggtagaca tccccggcga cacgctgcgc ggctcgtcac gcatgttgtt taattgtgcg
     4441 cgcagagtag cggagagttt cggataatta tcaatgactt cttgatattg cttgatgcgc
     4501 tcaagggaac cttttcgttc ctcaagcgca tttaaggcag actggagtgc ctctacgact
     4561 tctggctgcg cgggtttcgc cgcttttgcc tgctccagtt cctgggtgat ttgtttgcta
     4621 tcgggggccg tcgcggcgta cgccccccaa ctgaggcacc aggccatcag aaaagtgata
     4681 atcaggcgca cgtcagcgtt tcctttgatg gattagacct ggtctttttt atcgtcaacc
     4741 aatgggctgg cgtcgtgttc tgcttcgatc tcttcagcag gaagcggggc gggttcagcg
     4801 tctggcgtaa caaaggtttc ggtagatact gccagcggct ggccaatttt cgtgacagac
     4861 aggctttcca gttgctcaac cagattcact ttacccggtg caaacaggtt gataacggta
     4921 gagccaagtt taaagcgacc catttcctgg cctttcagca gcgccacaga accgtcgttt
     4981 tccccggcag gccaggtcca gcgcttgatg atgccttcgc gcggcggcgt aatggtgccc
     5041 gcccagaccg tctcaatgct gcctacaatc gtcgccccga ccagaatctg cgccattggg
     5101 ccaaattcgg tatcgaacag gcagatcacg cgttcgttac gggcaaacag attcggcacg
     5161 ttctgagccg tgagatggtt aacggagaag agatcgcccg gcacgtagat catctcacgc
     5221 agaataccgt tgcacggcat gtgtacgcgg tggtagtcac gcggggagag gtaagtggtc
     5281 acaaacgtac cgttgcggaa caggtccgcc atcagatagt tgcctgccag cagggcttcg
     5341 aggctgtagt tgtggccttt ggcttgcagg attttatctt cttcgatttt acccaactgg
     5401 ctgataacgc catcggcagg catgaccagg acattcggat cggtatcgat tgggcgtact
     5461 tcgtcacgca gcggacggac aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc
     5521 ttttgcgcct ctttcatgtc gaccttgtag tatttaacga acagatcgat aaccagtttt
     5581 gtcagccatc ctgcccgctt gcttgcgccc caacccgcca ggcgagtaag ccatagtttc
     5641 ggcagaatgt actgtagcga aagtttaaat gaatttaaca aggtagcctc caggccattg
     5701 ttttgtcgtt cctgatccgg cctaaatgcc ggatcctgaa aaaaagggga cgattctaac
     5761 gacggttagc ttaattgtca gtcatccgta tcagaaaagt ttttacgcgt ttttacctgc
     5821 gccatgcttt ccagaatacg gtgatagttt tcgaaacggg tttccgcgat ttttccttcc
     5881 tcaaccgctt cccggatagc gcagcccgga tcggtatcgt gtttgcaatc gcgatattta
     5941 cacagaccta aatagtcatg gaattcgaca aagccctgag tgatttgttc cggctccagg
     6001 tgccagaggc cgaactcacg cacaccaggg gaatcaatca catcaccgcc gtgcgggaag
     6061 tgatacaggc gtgcggcggt cgtggtgtgc tgaccaagcc ccgagttgtc agagacatcg
     6121 ttggtcagga tctctttttg cagccccagc aacgcgttca gcaggctgga tttgccgacg
     6181 ccagactgcc cggcaaaaat gctgatgcgc ccggtcaacg cctcttccag cggttttagc
     6241 ccatcctgag tatggctgga aaccatcaat acgcgataac cgatattgcg gtagatatcc
     6301 atctgttcgt tgacgaacgc catgccttcg tcgtccagca ggtctatctt gttgagcaca
     6361 ataatcggct caatctgcaa ggtttcgcag gccaccaggt aacggtcgat aatattgagc
     6421 gacagctccg gcaaaatggc ggagacaatg acaatctggt cgatgttggc ggcaataggt
     6481 ttcacgccgt cgtagaaatc cggacgcgtc aacactgagg tgcgctcatg caccgcttcc
     6541 acgatccctt tgacatttac gccttccgcc gccggtttac ccggacgcca gactacgcgg
     6601 tcgccggtca ccagcgaacg gatggtacgg cgaatattgc agcggtgaac gtcgccatcg
     6661 gcggattcca catcagcgtg cataccaaag cggctgatga cgataccttc atcaggctcg
     6721 ccaaacagat tgtcgtcgta gtcgggcttc tccttagacg ttttaagacg acgctggtga
     6781 ttggcgttca cgcggcgctg ctggcctttg gagagtttat ttttactcaa tcgtactgac
     6841 tcctggtcgc ccctgatggg caaaacatct atgatacacg caattgtgga tcaatatagt
     6901 cactgtgaat gggtggaaaa tagcatgagt gccaatgaaa acaacctgat ttggatcgat
     6961 cttgagatga ccggtctgga tcccgagcgc gatcgcatta ttgagattgc cacgctggtg
     7021 accgatgcca atctgaatat tctggcagaa gggccgacca ttgcagtaca tcagtccgat
     7081 gaacagctgg cgctgatgga tggctggaac gtgcgcaccc ataccgccag cgggctggtg
     7141 gagcgcgtga aagcgagcac gatgggcgat cgtgaagctg aactggcaac gctcgaattt
     7201 ttaaaacagt gggtgcctgc gggtaaatcg ccgatttgcg gtaacagcat cggtcaggat
     7261 cgtcgcttcc tgtttaaata catgccggag ctggaagcct acttccacta ccgttatctc
     7321 gatgtcagca ccctgaaaga gctggcgcgc cgctggaagc cggaaattct tgatggtttt
     7381 accaaacagg ggacgcatca ggcgatggat gatatccgtg aatcggtggc ggagctggct
     7441 tactaccgcg agcattttat caagctgtaa aatttttgct ggtcacgtaa gcgccggaat
     7501 ccacttgcgg cggcgtgatt ttgacgctaa attgaacgtc ttgctgataa tttcagcact
     7561 tgagataaaa actcaaaaaa aaatttttag gggggttgca gagggaaaga tttctcgtat
     7621 aatgcgcctc ccgtaacgac gcagaaatgc gaaaattacg aaagcaaaat taagtagtac
     7681 gcgggaatag ctcagttggt agagcacgac cttgccaagg tcggggtcgc gagttcgagt
     7741 ctcgtttccc gctccaaaat ttgaaagtgc tgtaaggcac agaccaccca agcgggaata
     7801 gctcagttgg tagagcacga ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc
     7861 cgctccaaat tcttctctca ataaaatatc cacagcgacg cgatgcgtta ttgctggttt
     7921 ttgttgtctc tgacaaactc ttgtaaacag agttatccac agcctcaggc cgtaatctta
     7981 atttca
//
LOCUS       DBFIFS010000199         7972 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.199, whole genome shotgun sequence.
ACCESSION   DBFIFS010000199 DBFIFS010000000
VERSION     DBFIFS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.199"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(337..909)
                     /gene="hdeD"
                     /locus_tag="N7A07_003873"
     CDS             complement(337..909)
                     /gene="hdeD"
                     /locus_tag="N7A07_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU2478449.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1164..1496
                     /gene="hdeA"
                     /locus_tag="N7A07_003874"
     CDS             1164..1496
                     /gene="hdeA"
                     /locus_tag="N7A07_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU2478450.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1612..1938
                     /gene="hdeB"
                     /locus_tag="N7A07_003875"
     CDS             1612..1938
                     /gene="hdeB"
                     /locus_tag="N7A07_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU2478451.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2002..2649
                     /gene="yhiD"
                     /locus_tag="N7A07_003876"
     CDS             2002..2649
                     /gene="yhiD"
                     /locus_tag="N7A07_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU2478452.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2701..3471)
                     /locus_tag="N7A07_003877"
     CDS             complement(2701..3471)
                     /locus_tag="N7A07_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HGU2478453.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3468..4460)
                     /gene="shuU"
                     /locus_tag="N7A07_003878"
                     /gene_synonym="chuU"
     CDS             complement(3468..4460)
                     /gene="shuU"
                     /locus_tag="N7A07_003878"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HGU2478454.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEPQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4509..5132)
                     /gene="chuY"
                     /locus_tag="N7A07_003879"
     CDS             complement(4509..5132)
                     /gene="chuY"
                     /locus_tag="N7A07_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HGU2478455.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5132..5626)
                     /gene="hutX"
                     /locus_tag="N7A07_003880"
     CDS             complement(5132..5626)
                     /gene="hutX"
                     /locus_tag="N7A07_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HGU2478456.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(5639..6976)
                     /gene="hutW"
                     /locus_tag="N7A07_003881"
     CDS             complement(5639..6976)
                     /gene="hutW"
                     /locus_tag="N7A07_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HGU2478457.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(6996..7910)
                     /gene="shuT"
                     /locus_tag="N7A07_003882"
     CDS             complement(6996..7910)
                     /gene="shuT"
                     /locus_tag="N7A07_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HGU2478458.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         2123 a         1917 c         1942 g         1990 t
ORIGIN      
        1 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
       61 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
      121 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
      181 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
      241 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
      301 atcaagtgac tatgatccgg gtgacaaccg ggataattat tgctgcttaa cgaacaaact
      361 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
      421 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
      481 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
      541 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
      601 gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
      661 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataatc caacaataag
      721 cgcaatgccc gaacagatta ataatgcacc tactactgtg cttaaaatat cgccagaaac
      781 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
      841 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg ccttatctat
      901 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
      961 aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg
     1021 catctgtaac tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg
     1081 acatatacag aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc
     1141 actttatagt tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc
     1201 ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac
     1261 cggtcaactc ctggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg
     1321 cagttggttt tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg
     1381 ttcagggtat tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag
     1441 ccaactttaa agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc
     1501 gggaatgcgt tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa
     1561 gcagctattc ctcctgttca tatataatct ctatattgaa tgggttacaa aataaatatt
     1621 tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac
     1681 gcacaatctg cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt
     1741 gatctgaatc caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta
     1801 tataaaggtg gcgatacagt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg
     1861 atcgaatact gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct
     1921 aatgacttgc cgaattaatg aggtacaagt aaaaaggagt ggcaagttga gccatcttgc
     1981 tgctcctttt tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca
     2041 attgcctgtg gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc
     2101 actcatgtat taattggcat gggaagcgca ctgtttatga ttgtttcgaa atatggtttt
     2161 gctgacgtgc tgtctttaga tcacgtcgga ctggacccca gccgtattgc tgctcaggtg
     2221 gtgacgggcg tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta
     2281 ggtttgacga cggcagcgga tatctgggtg accgccgcca taggtatggt tattggcagt
     2341 ggtatgtacg aactgggtat ttatggttca gtgatgacct tgctggtgct ggaagtcttc
     2401 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     2461 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     2521 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     2581 acgtcgatag aagatctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     2641 attagctaat ctgtatagtt atttattcgc aggagtacca gtgcggtact cctgttgctt
     2701 ttaatgatcg aggacgatca gtggtgaatg gttggcagga tctttaagta cggtaatatc
     2761 cgcgccgtag agcattgtca gcgcctgctg tgtcaatacg tcctgtggtt taccatttgc
     2821 gataaccttt cctttttgca ttaatacaac acggtctgca taatgcgcgg caagatttag
     2881 atcgtgcaat atgcagcaga cattgaactg ccgctcgtga actaactggc gtaataaacg
     2941 aaacaagtgc tgctgatggt gaatatccag cgccgaagtt ggttcatcaa gaaataacca
     3001 tttcggtgag ggagttggtt cccagagttg caccagcagg cgcgctaatt gcacgcgctg
     3061 ttgttcacca ccggataatt gccgatagtc gcggtttgct aacgcctgac agtcacataa
     3121 cgccattatt tgtgctgttt cattatcctg attaccagta cgatgcggat gccgacccat
     3181 ctggataact tcttgtacgc tgaatggaaa cgccatgtgg ctattttgac gcatcacggc
     3241 ccggtgtttc gccagttcag taatcgacca ttcatttaat ggcttattaa acaaccgaca
     3301 ttcgccgcta tcaggttgaa gatagccggt taactggcga agcagcgtag attttcccgc
     3361 accattaggg ccgagaatgg caacaatttc cccaccggga aaagtcagcg agacattatc
     3421 agtcagacgc cgcccctgaa ggctataaac cagattctgg gcgctgatca tgtgatcctc
     3481 cgatttcgca gaatcaacca cataaaataa gggccaccaa gcaggctggt taataatccc
     3541 acgggcattt ctgctggctg taccagcgtg cgggcaagcg tgtctgccat cagcaataaa
     3601 caggctccgg cgagggcgga acaagggatg agccagcggt gatttgctcc ggtagtcatg
     3661 cgaatcagat gcgggatcac caggccaata aagccgataa tgccgcttac cgatacggca
     3721 gcaccaacga gcagtgagct cactaatagt aattgctggc gtttacgctt aacgttcacg
     3781 ccgaggtaat gggcttcttc atcaccgagc tgcagtaaat tcagcgttcc ggcgagacaa
     3841 gttgttgcga taatggccgg aaggatgaat gaactggcaa ccaataacgt cggccattgc
     3901 gcctgtccaa gattgcccat catccacaat gtgagttgtc gtagctgcgg ctcatcgcca
     3961 atatagctca ggatgccgat cgctgcgccg cacagggcat tgatggcgat accggcgagt
     4021 aataattgca tcatgctgcc atcgcgatgg cgctgcgtga tgagaaaaat gatggcgcag
     4081 actactaaac ttccggcaat ggcaaacacc atttgctcat agagcaccag caccactggg
     4141 aaggagaagg gcagcacaat tgcaacgcca accattaatg cagaaccgct actgacgccg
     4201 agtaatcctg ggtcagccat tgggttgcga aaaagcccct gcataattac ccctgcggtt
     4261 gccaacgctg cacctaccag caccgccagc aaaacccgtg gtagacgaat attgagccag
     4321 atatcgcggt attcctcatc aaaaagggca tcaaaactga tctttaacgc tccctgacta
     4381 gcaccgaaca gcaccagggc aagcaataat agtgatagcc ccataaagac cctggcggat
     4441 gaaaatgaat ccttgagcac gcaaaactcc gttacgacat tacggcgcgg acatgccgcg
     4501 ccggggtatc acgccggttt caggtccggt tcaatcagac tgtagacctg ctggttaagt
     4561 gctggcgcat ttgccagttc atggatatgt gcggcgacat cggcacggcg gacaaaacca
     4621 tgacactcct gattttgtat ccgttgtgct ttgcctgttg ctgcgccgtc aagcagacca
     4681 ccagggcgga gaatggcgta gtctagctgg ctggtttgca gccagctttc cgccagcgtt
     4741 ttttcccgta ccgcctgacc aaacgcagcc tttgcccgct ccgataaaaa cggccagcta
     4801 tcgccgcatc ccaaagaggt caccagaatc atgcgactga tgccggcctt ttcggcctca
     4861 tcaatcactg ttctgtgcgc cagataatcc tgagctcctc ccattgttga gatgataagc
     4921 gcatccgggc cagctgcgcg gcaagcagcc gcgactacgc tggcatcaca ggcgtcgcct
     4981 gtaaaaacct gtacgccttg ttgcgccagt ttcgtggcgg catcggcatg acgaatgaca
     5041 gcaacgaccg gacgttgttc cgccagcgcc agttcaagcg ttctggcacc cacacctttt
     5101 ccgcctgcgc caaagagtag ccacggcgtc atcaggcgtg ttcctttaac gatgccgcca
     5161 gagtatggaa ggcgctgact tgttcgctca ggagctgacg atggtcatcc ctgccgagga
     5221 agattttcag catggcgctg ccttctttgt tgaaaaagag aatggacgcg gtgtccatgc
     5281 ccataaattt acgttcgatt aaggcaatat gcgtgcagtt ttctgctttg atatgcccgg
     5341 acatgccgtg ctttccgcgt aggttgaaat agccgtggcg atggaaaccc gaaggcagtt
     5401 cgccgctaaa ttcgaggatt acatcggcag tatgtactaa cgtggtgacg ttgccccatt
     5461 cgcagacggt atcccagaca gtatcgaatt tatcgcctgg cactacggta gaggagggta
     5521 gatttctcac tacctccagc agtgtggtgt tgtattgttc ggctaccacc tccagcgtac
     5581 cgtccggctc cgttttcaaa aattcttgta acgagacatg gctcataaat cactcctgtt
     5641 atggtttttc acgcataatc gctggcgcat taagtacctg aattagttca ttaagagact
     5701 gcaaaatatt gcttgcccag aaacgacctt cattagtcag ccgcaggcaa gttgaagcat
     5761 cgcgacttaa gcctttttgg tgccattgag ccagtaacgg cgcgagtttt tcggcatgtg
     5821 gcgtaagttc atccagcggt acgcgcgctg tttcaacacc tgactgcaag gtatgacgcc
     5881 attgcgcatt acgttcggcg ttacgcatga tcaacatcag cggttttttg cctgccgcga
     5941 cggattcatg ccaggtctga agattgcgtt cgttcatcca ggagtaacca ttaatcgaac
     6001 cgccggctcc ggaaccaaag gccagacaat cggcaccttg tttaatcagc aggttataga
     6061 gattgcgttc gcgtgtggta cggccccagt ggctgttact gatgcagcgc caaccggcat
     6121 cgtccataaa atcacatcct tgcagataaa gatcgcgacg ttctgccggt gagggtacgg
     6181 tagtacgccc attttccacg gccttgccca gcggtgtatt ggacaggaca ttgagcgcat
     6241 agagatcgac gccgtcgaga ccgatatcgc gggcaatagc cagatcttct ccccaggttt
     6301 gcgcatcctg acccggcagg ccaaacagca ggtcacagac caccgcagca cggtcacgtt
     6361 tgaccaggct ttccataaac gcaatagcgg ttgggccatc cgaggtgcgg gccattttct
     6421 tgcggatttt gctgttaaac gactggatac caattgagaa gcggtttgct cctgcatcaa
     6481 ggcaagcatc gattcgctca gcgtcaaaat tcaatacccg tccttcgata gtgatttcac
     6541 aatcaggggc cagtggcagc ttttctcgta atgtggtgat gattctggcc aaatcgtgtg
     6601 ccgaaagtgc ggaaggcgta ccgccaccga aatagaccgc gtggatgggg gcagactgat
     6661 gcaatacgct gtccgcttcc atttctattt cacgaatcag ggcgtcggta taatgagcac
     6721 acgcatcttc attaaaacgg ttctgataaa aaccacaaaa cgtgcagtgt gtcgcacaaa
     6781 aaggaatgtg taggtaaacc agtcgcttac gaggtgatgc cgtttgattg atcatctctt
     6841 gccaggtctg tgccagttgc tctttggcaa ccggaatggc tccacgaaac ggcatcatgg
     6901 cgcgcctgtc tttaaatggc tgatcaccgt ctaaagcaaa atgtggcgtg agatccaggg
     6961 tgttatttgc gttcattcgt ctgatttaaa tccatttatt gcggccaaag ctgctggtgt
     7021 aaagactcaa cgacatcagc aatacgcgga cccattccca gaatcaggtt ttgatcaacg
     7081 gtgataatgc gctggttttt ccatgcggca gtgtgggtaa ttccagcaat agaacgtagc
     7141 cgattaatat cgccatcgac catttgtgag gttacgacaa tcacttctgg attagccgca
     7201 atcagtgatt ctgcactgta gcttttgtac tgctggtggg tagcgacgtt ttctgctccg
     7261 gcaagcgaaa gaatggcgtc cgcgacgctg cctttaccgg caacttgtgg cgcacttccg
     7321 cctgcggaga gaataaacat ggctttaacc ggggcttttt tggtcgccac gttttgctgt
     7381 actcgctcca ggcgttggct gatttgtgtc accagcgcct cgccttgttc aggaacctgt
     7441 agcgtttttg ccagttggcg aatgttggcg tacatctgct gaagggtggc aggcacacgc
     7501 ggcagggtga cgacgttgac tttctgcgct cgtagttggt cgagcacaat ttgtggcccg
     7561 gcatcctgcc aggtaattac gctatccggg cgaagtgaca aaatgccttc gctgctgagt
     7621 tgtttccagt agccaatatg cggcagtttg gcggtttctg gtggataaga tgtcgtttca
     7681 tcgacaccaa ccacgcgttc gccagcgccc atcgcgtaga ttagctccgt cagcgatcct
     7741 cctgcgacca cgatacgttc ggcagccgtt acgctaaaag cccagctaag agccagtact
     7801 gccgtaaaca attttttacg ttttactgcg gtcttcgcgg cagaggccac ggctgaaata
     7861 cataacgtca gtggggaaaa aagaaacggc ctggtgatga tccttggcat aatatgagaa
     7921 ctcttatcaa attgagaatg atcatgatta tcatttgagt tatatatgaa at
//
LOCUS       DBFIFS010000200         7964 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.200, whole genome shotgun sequence.
ACCESSION   DBFIFS010000200 DBFIFS010000000
VERSION     DBFIFS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.200"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..234
                     /gene="yafQ"
                     /locus_tag="N7A07_003883"
     CDS             <1..234
                     /gene="yafQ"
                     /locus_tag="N7A07_003883"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU2478459.1"
                     /translation="VKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGY
                     RDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            390..1130
                     /gene="dpaA"
                     /locus_tag="N7A07_003884"
     CDS             390..1130
                     /gene="dpaA"
                     /locus_tag="N7A07_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU2478460.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1101..1868)
                     /locus_tag="N7A07_003885"
     CDS             complement(1101..1868)
                     /locus_tag="N7A07_003885"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU2478461.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1973..2551)
                     /gene="lpcA"
                     /locus_tag="N7A07_003886"
     CDS             complement(1973..2551)
                     /gene="lpcA"
                     /locus_tag="N7A07_003886"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU2478462.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2791..5235
                     /gene="fadE"
                     /locus_tag="N7A07_003887"
     CDS             2791..5235
                     /gene="fadE"
                     /locus_tag="N7A07_003887"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU2478463.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5278..5724)
                     /gene="ivy"
                     /locus_tag="N7A07_003888"
     CDS             complement(5278..5724)
                     /gene="ivy"
                     /locus_tag="N7A07_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU2478464.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5905..6675
                     /gene="yafV"
                     /locus_tag="N7A07_003889"
     CDS             5905..6675
                     /gene="yafV"
                     /locus_tag="N7A07_003889"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU2478465.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(6793..>7964)
                     /locus_tag="N7A07_003890"
     CDS             complement(6793..>7964)
                     /locus_tag="N7A07_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HGU2478466.1"
                     /translation="SIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEWQ
                     EIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITDG
                     EIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLNL
                     LDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNYK
                     GDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAEG
                     VSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISGI
                     KKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1919 a         2011 c         1972 g         2062 t
ORIGIN      
        1 gtaaaacttg cacaaaagcg tcataaggat atgaataaat tgaaatatct tatgacgctt
       61 cttatcaata atactttacc gcttccagct gtttataaag accacccgct gcaaggttca
      121 tggaaaggtt atcgcgatgc tcatgtcgaa ccggactgga tcctgattta caaacttacc
      181 gataaacttt tacgatttga gagaactgga actcacgcgg cgctctttgg gtaaagcccg
      241 ctgccgatga aatttgtaaa ccctgcgccg acaggcttgc gtaaaactct tttggtgata
      301 atgtgagcct tcccaatcca gactattccg atttgggatc cattttgccg tcctggcgtg
      361 tgtaaccgtt ttatcaagga ataagcagta tgcgtaaaat cgcattaatt cttgcgatgt
      421 tgttgatccc gtgcgtttcg tttgccggtt tgctgggcag cagtagctcg accacgccag
      481 tgagcaaaga gtataagcag cagttgatgg gatcccctgt ctacatccag atcttcaagg
      541 aagaacgtac gctcgatctc tacgtcaaaa tgggcgagca atatcaactg ctcgacagct
      601 ataaaatctg taaatattcc ggcggcttag ggccaaaaca gcgtcagggc gatttcaaaa
      661 gcccggaagg gttttatagc gtccagcgta atcagttaaa accagacagc cgttactaca
      721 aagcgattaa tattggtttc cccaatgcct atgaccgtgc gcatggttat gaagggaaat
      781 acctgatgat ccacggcgat tgtgtttcca tcggctgcta cgcgatgacc aatcagggca
      841 ttgacgagat tttccagttc gttactggcg cactggtatt tggtcagccg agcgtgcaag
      901 tgagcattta cccgttccgc atgaccgacg ccaatatgaa gcgccataaa tattccaact
      961 ttaaggactt ctgggagcaa ctgaagccag gctacgacta ctttgagcaa acccgtaagc
     1021 caccgaccgt ttctgtggtc aatggccgtt acgtggtcag caagccgtta agccacgaag
     1081 ttgtgcaacc acaactggca tcaaactaca cgctccccga ggcaaaataa gcgccattcg
     1141 cctggcataa tcttttgcca ggtttcattg cccgtcagcg gctgtgtcgc aatcaccgtg
     1201 accacatcat tcggtgtggt ctgcgagctg aagtcgattt ccacatcctg atccagcaac
     1261 gttgccacgc caaacggcgc gcggcgggtg atccagtgca gattggtcga gcaatacgcc
     1321 attacatagc gcccatccga aagcagcatg ttgaaaacgc ccttctgccg cagttcatcc
     1381 gccagtgagg cgatatattt aaataccgcc gccatattgc ccggcgtacg cggataacgc
     1441 tgcgttaatt tatacagcag ccagcaaaag gctttttcgc tgtcggtttt acctaccggg
     1501 cggaagttgc cggtttccag tgatttatag cccgtcagtt gtccgttatg ggcataagtc
     1561 cagttacgtc cccataactc gcgggtaaac gggtgagtat tttccaacgc cacctcgccc
     1621 cgatttgcct ggcgaatatg agccacaacc gaacaggatt ttatcgggta gtcctgaaca
     1681 agtttggcaa tgggggaatt aaagctgggt tgtggatctt taaatgtgcg acagccttta
     1741 ccttcgtaaa aggtaatgcc ccagccatct ttatgtggcc cggttccacc accacgctgc
     1801 accagcccgg tgaaactaaa gcaaatatcg gtagggacgt tggcgctcat cccgagcaat
     1861 tcgcacatcg gtaaccccca caacagcggg agcgcatggc ccccggcgaa gattgactta
     1921 agaattttca ggccggatgc ggcgtaaacg cattattcgg cctacgccag acttacttaa
     1981 ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga atttcctgaa
     2041 tgcggtcggc ataaccaaaa tgtggaacgc gaatttcaat atccgccgta ccagccattt
     2101 tgccgccatc tttgccggtc agggtaatca ctttcattcc cttctcacgc gccgccgcga
     2161 tcgctttgat cacgtttgca gagttaccag acgtggagat ccccagcagt acatcgcctt
     2221 cgcggcctac cgcttcaaca tagcgggaga aaatatcatt gaaaccaaaa tcgttaccga
     2281 cgcaggaaat atggctgacg tcagagatgg caatcgccgg gtagcccgga cggttttcac
     2341 ggtagcgacc ggtcagctct tcggcaaagt gcatggcgtc gcaatgggag ccgccgttgc
     2401 cgcaggaaag caccttgcca ccggctttaa agctgtctgc taacaggacc gccgctcgct
     2461 gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc gccgcttcgt
     2521 tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata aatgtaatag
     2581 acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt gctatgacca
     2641 ggactttttg ccctgaagtg tagataaaaa cagcaacaat gtgagcttcg ttgtaattat
     2701 attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc acaagtggtc
     2761 agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct cgctacagtt
     2821 gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag tctgattttg
     2881 ctcgcctgga cagctgccct cggcgtcgct ggtctgtggt cggcgtgggt actggtgcct
     2941 ctggccatta tcctcgtgcc gtttaacttt gcacctatgc gtaagtcgat gatttccgcg
     3001 ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg
     3061 attgatgcgg gtaccacctg gtgggaaggc gacttgttcc agggcaagcc ggactggaaa
     3121 aagctgcata actatccgca gccgcgcctg accgcagaag agcaagcgtt tctcgacggc
     3181 ccggtagaag aagcctgccg gatggcgaat gatttccaga tcacccatga actggcggat
     3241 ctgccgccgg agttgtgggc gtatctgaaa gagcatcgct tcttcgcgat gatcatcaaa
     3301 aaagagtacg gcggcctgga gttttcagct tatgcccagt ctcgtgtgct gcaaaaactc
     3361 tccggcgtca gcgggatcct ggcgattacc gtcggcgtgc cgaactcatt aggccccggc
     3421 gagctgctgc aacattacgg cactgacgag cagaaagatc actatctgcc gcgtctggcg
     3481 cgtggtcagg agatcccttg ctttgcactg accagcccgg aagcgggttc cgatgcgggc
     3541 gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca ggtgctgggg
     3601 atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac tgtgcttggg
     3661 ctggcgttta aactctccga cccggagaaa ttactcggcg gtgcagaaga tttaggtatt
     3721 acctgtgcgc tgatcccgac caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg
     3781 ctgaatgtgc cgttccagaa cggaccgacg ctcggtaaag atgtcttcgt accgatcgac
     3841 tacattatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc
     3901 tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg
     3961 ctggcaacgg gcgcgtatgc tcacattcgc cgtcagttca aaatctctat cggtaagatg
     4021 gaagggattg aagagccgct ggcgcgtatt gccgggaacg cttatgtaat ggacgctgcg
     4081 gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc
     4141 gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat ggatattacc
     4201 ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta ccagggcgca
     4261 ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat gatcttcgga
     4321 caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc gaagaacaat
     4381 gacgtcaacg tgttcgataa actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa
     4441 gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat
     4501 gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc cctgctttct
     4561 gatgtctcga tggcagtgct gggcggcagc ctgaaacgtc gtgagcgtat ctcggcacgt
     4621 ctgggtgata ttttaagcca gctttacctc gcctctgccg ttctgaagcg ttatgacgac
     4681 gaaggccgta atgaagccga tctgccgctg gtgcactggg gcgtacagga tgcgctgtat
     4741 caggcagaac aggcgatgga cgatttactg caaaacttcc cgaaccgcgt ggttgccggg
     4801 ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccgtc tgacaagctg
     4861 gatcataaag tggcgaagat tttacaagtg ccgaacgcca ctcgctcacg tattggtcgc
     4921 ggtcagtacc tgacgccaag cgagcataac ccggttggtt tactggaaga ggcgttggtg
     4981 gatgtgattg ccgccgaccc gattcatcag cggatctgta aagagctcgg taaaaatctg
     5041 ccatttaccc gtctggatga actggcgcac aacgcgctgg cgaaggggct gattgataaa
     5101 gatgaagccg ctattctggt gaaagcggaa gaaagccgtc tgcgcagtat taacgttgat
     5161 gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa
     5221 gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat ccgctaatta
     5281 tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg cgaacagcac
     5341 cgttttacca tcaatcgaaa gcgcatcgtt cacattcaac caggtcagtt tctcttgcga
     5401 cgttttctca tcaatagtcg agaacagccc cgtcatctga ttagatttct cggaccacat
     5461 cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca cctgatacgt
     5521 ctcatctccc aacgttacgg tttgtgcagg ggtataagta ccgcctttca tcacccaggc
     5581 aggcagctta tgcccttgca ccatctgatt aaatgcagct ttggtggttt cgccctttgc
     5641 aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga ccagtgcggc
     5701 gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca tgttattaac
     5761 ctcctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa tatcagcatt
     5821 tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc
     5881 cttcgcataa aggagttgta caccgtgcct ggtttgaaga ttacgctttt gcagcaacca
     5941 ctggtgtgga tggatggtcc tgccaacctg cgtcattttg atcgtcaact ggaaggtatt
     6001 accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc catggaagcg
     6061 gctgcttcgt cgctagcaca agatgacgta gtgaactgga tgacagccaa agcgcagcag
     6121 tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc ggttaaccgc
     6181 tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca tctgttccgc
     6241 atggcagatg agcatctgca ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc
     6301 ggctggcgta ttttgccgtt ggtgtgctac gacttacgtt ttccggtgtg gtcgcgcaat
     6361 ctcaacgatt atgacctcgc catctacgtt gccaactggc ctgctccgcg ctctctgcac
     6421 tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat
     6481 cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt gattaatcca
     6541 caaggcgaga ttatcgctac tgccgacgcg catcaggcaa cgcgcattga tgcggagctg
     6601 tcgatggtgg cattgcggga atatagagaa aagtttccgg catggcggga tgcggatgag
     6661 tttaggttgc ggtgagtggg ggctgtattt agggcgggga attcagaaag ggatgtttag
     6721 tgtgctgagc gagagttaaa taataaaaaa ctgggaagat ttaataatcc ccccgcaggg
     6781 caagagcatg aattaaatct gtgttaaatt tttcattttg tcagttcgaa acccaagctc
     6841 tgagcaggat gcaagcactt cttctatata acatgtgttt aacgcaacct tctttctttt
     6901 cctcttcacc ccacccttaa tgtctttgca atctcttaat aaattcagtg ccatcttctt
     6961 tattccagat attatttcgg ctgcgtttcc tcttcttatc cggctggcat cttcattcat
     7021 ttttacatct aacacccaat gaagactgtg ctcgatcagc cagtgcgctc tgatagcatg
     7081 tgcaaattct ttagcatcca tatcctttga tgaaatataa tatcggatgc ttacaccttc
     7141 tgctgattta tcttctttct tctgcctgaa tgacaatgct acacaaagct tttttaatcc
     7201 cttccattcg aattcaaaat cacaaaattc aggcgttacg ttactgacaa tatgcaaacg
     7261 tgtttctttt cttccatgac ttatctcctg cgtactaaac gaatcgcctt tataattaga
     7321 aaacacattt acaggaaatt tttcctcgaa tgcatgatgt aatttcccct gattgccttt
     7381 tactgccaga agataatctg cttttttatc tttgatcttc gaagcgatat ctttctgaca
     7441 gcccatagca tcaatggtta tcaaattttt ctttaaatcc agtaggttaa gcaactctgg
     7501 aatggctgta atctcattac ttttggcttc cgtttttacc tgccccagta caacaccatt
     7561 ttcgttcgag aatgcactca ccatatggat tgctcctttt ctttttccct tatcaaagga
     7621 gcctcttatg gtctttccat ctattgctat aatttcacca tcagtgattt catggcactc
     7681 ctgcatccat tcaataaata tcttttcaaa ggccaaactg tcaatgttac tcacaacgcg
     7741 tgcaatggtg tcatcgaccg gaatgccatt atcaaaatca ccatatttct ttagccattc
     7801 aagtctttca tgtccaaaat cttcaatttc ctgccactca tcggcacctg caattactgc
     7861 acatacggtg agaaacaaaa tatcagataa tttatgttta acctttcctt gttgtcgtat
     7921 atcagggatc actgaaatat aatcaagcaa actttgaata ctca
//
LOCUS       DBFIFS010000201         7959 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.201, whole genome shotgun sequence.
ACCESSION   DBFIFS010000201 DBFIFS010000000
VERSION     DBFIFS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.201"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            170..694
                     /gene="lpfA1"
                     /locus_tag="N7A07_003891"
     CDS             170..694
                     /gene="lpfA1"
                     /locus_tag="N7A07_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HGU2478467.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            759..1445
                     /gene="lpfB"
                     /locus_tag="N7A07_003892"
     CDS             759..1445
                     /gene="lpfB"
                     /locus_tag="N7A07_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU2478468.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1474..4047
                     /gene="lpfC"
                     /locus_tag="N7A07_003893"
                     /pseudo
     CDS             1474..4047
                     /gene="lpfC"
                     /locus_tag="N7A07_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            4062..5117
                     /locus_tag="N7A07_003894"
     CDS             4062..5117
                     /locus_tag="N7A07_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478469.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5122..5652
                     /gene="lpfE"
                     /locus_tag="N7A07_003895"
     CDS             5122..5652
                     /gene="lpfE"
                     /locus_tag="N7A07_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU2478470.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5907..7598
                     /gene="eptB"
                     /locus_tag="N7A07_003896"
     CDS             5907..7598
                     /gene="eptB"
                     /locus_tag="N7A07_003896"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU2478471.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7690..7766
                     /locus_tag="N7A07_003897"
     tRNA            7690..7766
                     /locus_tag="N7A07_003897"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7724..7726,aa:Pro,seq:cgg)
BASE COUNT         2019 a         1840 c         2063 g         2037 t
ORIGIN      
        1 gaaaatgtaa tgcaatgatt tttttaaggt ttgaaattgg atgcgctatt aatatattat
       61 tcgtataatt ttattattcg tatttcattg tataatcctt ccttcaaagt tgtcgatttt
      121 aatacatcaa gattttcttt ttaatgtaat tttttaaatg gagtttttca tgaaaaaggt
      181 cgtttttgcc ttaaccgcac tggcgttaac ttctggcact gtattcgcag cagagtctgg
      241 tgacggcacg gttaaattta caggcgagat cgtggattca ccttgcgtac tgtccgttga
      301 ctctcagaac caggaagttg tgttgggtca ggtacaaaaa tctgtatttg ctgcggttgg
      361 tgacaaatcc ccggcaaaac ctttcgaaat caaactggaa gattgcgata ccaccacgat
      421 gaaaaaagcc aatgtgagct ttagtggtgt tggtgatgca gataaatctg atctgatttc
      481 tgttagcact gaagcgggcg cggccaaagg tgttggtatc ggtatttatg ataacagcaa
      541 taccctggtc gctcttaacg gtggtaaagc aagtgttgat ctgagtaaag gccagacggt
      601 tctctacttt accgctaact acgtttctac tctggcaacg gtaacgaccg gttacggcaa
      661 tgcgcaggtc gatttcaacc tgtcttacga gtaatcgtcc gttttacaaa tggcggcgta
      721 attgccgcca ttagtcgtca aagttcatgg atcgaatgat gaaaagtaaa tttgttgcgt
      781 tggctctctc tctgttcctt tcacagtcag ttctggcggg tggcgtcgga cttagctcta
      841 ctcgcgtaat ttatgatggc agtaagaaag aagcctcgct gaccgtacag aataaaagta
      901 agacggaaga gtttcttatc caatcctggg tggatgatgc cgcgggcagc aaaaaaacac
      961 cgtttattat tacgccccca ctattcaagc ttgatccaga aaagaacaat attttgcgta
     1021 ttgtgaatat tacgcccggc ctgcctcagg atcgcgaatc ggtctactgg gttaatgtga
     1081 aagccattcc atctaaaagt gacgacagcg aaaacaaaaa tgttttgcag attgccgttc
     1141 gcacccgaat caaacttttt tatcgcccgg caggtttaaa gggtgatgta aaaaccgcgc
     1201 caaatgaact gcgatttacg cgtaacggta atcagctccg cgttgataat ccgacagttt
     1261 ttaatattac ctttaatcaa ttttttgcga atgacaaaga aattgaaaaa gcaggcatgg
     1321 tacccgctaa aggggcatta aatatcacat tacccgccgg ggtaggtagc gtttctaaaa
     1381 ttaaatataa caccatcaat gactttggat cgtcagcgga aatgatcact aaaaatgttg
     1441 attgattaat atcttttcag gagaaatagc tcaatgtcac ggaagaccgt ttcacgtact
     1501 ttttcttcct tttctatctc tgtggtggcg gttgctgttg ccagcacgtt ttctgcacat
     1561 gccggaaagt ttaaccccaa attcctggag gacgttcagg gtgtgggcca gcatgtcgat
     1621 ttaacgatgt tcgaaaaagg ccaggaacag cagttacccg gcatctatcg tgtctctgtt
     1681 tacgtcaatg aacagagaat ggaaacccgc acactggaat ttaaagaggc cacagaagca
     1741 cagcgcaaag cgatggggga atcccttgtg ccttgcctta gccggacgca actggcagag
     1801 atgggcgtgc gcgttgaaag ctttccggcc ctcaatctgg tgtctgccga agcctgcgta
     1861 ccgtttgacg aaatcattcc cctggcctca agccatttcg acttcagtga acaaaagctg
     1921 gtattaagtt tccctcaggc ggcgatgcac caggtggcgc gcggcacggt gccggaatct
     1981 ttatgggacg aaggtatccc tgcgttgctg ctggattaca gttttagcgg gagcaacagt
     2041 gaatatgact cgacggggtc ttccagcagt tatgtcgatg ataatggcac tgttcatcat
     2101 gatgatggta aagatacgct aaagagcgac agctactacc tcaacttacg cagcgggctg
     2161 aaccttggcg cttggcggtt acggaattac agtacctgga gccattccgg tggcaaagcg
     2221 cagtgggata atatcggtac gtctttgagt agggccatca tccctttcaa agcgcaatta
     2281 acaatggggg atacggcgac cgcgggggac atttttgaca gcgttcagat gcgtggcgcg
     2341 atgttagcct ctgatgaaga gatgctgccc gacagccagc gaggctttgc acccatcgtt
     2401 cgagggatcg ccaaaagcaa cgccgaagtc agtattgaac aaaacggcta cgttatttat
     2461 cgcacctatg tgcagcctgg tgcctttgaa ataaacgatc tttaccccac tgcaaacagc
     2521 ggtgatctca cggtcattat taaagaagcg gatggcagtg aacaacgctt tatttagcca
     2581 ttctcatcgg tgccaatttt ccagcgtgaa ggccatctca aatatagctt tgccgcaggt
     2641 gaatatcagg ccgggaatta tgacagcgcc tcgccgcgtt tcgggcagct tgatctgatc
     2701 tacggtttac cgtgggggat gacggcctac ggcggcgtat taatctctaa taattacaat
     2761 gcatttacat tagggatagg gaaaaacttt ggttatatcg gggcgatttc cattgatgtg
     2821 acgcaggcta aaagcgaact gaataacgat cgcgatagcc agggacaatc ttatcgtttc
     2881 ttatattcca agagcttcga aagcggcacc gatttccgcc ttgcgggcta tcggtactct
     2941 accagcggtt tctatacctt ccaggaagcc accgatgtgc gcagtgacgc tgacagcgac
     3001 tataaccgtt atcacaagcg cagcgaaata cagggtaacc tgacgcagca attaggggcc
     3061 tatggctctg tttatttaaa tttaacgcag caggattact ggaacgacgc aggtaaacag
     3121 aacacggtat cggcgggtta caacggacgt attggcaagg tcagttacag tattgcatat
     3181 agctggaata aaagccctga atgggatgaa agcgatcgct tgtggtcttt caatatttcc
     3241 gttccactag gccgggcctg gagtaactat cgcgtcacga ccgaccagga tggtcgtacc
     3301 aatcaacagg ttggggtcag cggaacgctg cttgaggatc gcaacctgag ctacagtgtc
     3361 caggaaggct acgccagcaa cggtgtgggt aacagcggta acgctaacgt tggctatcag
     3421 ggtgggtccg gtaatgtcaa cgtaggctat agctacggga aagattaccg gcagctcaac
     3481 tacagcgttc gcggcggcgt gatagttcat agcgaaggcg tgacgctttc ccaaccgcta
     3541 ggcgaaacca tgacgctcat ctccgtaccc ggtgcgcgca atgcccgcgt ggtgaataac
     3601 ggcggcgttc aggttgactg gatgggtaac gcgatcgtgc cttatgccat gccgtatcgt
     3661 gaaaacgaaa tctcactgcg tagcgattcg ttgggtgacg atgttgacgt tgaaaatgcg
     3721 ttccagaaag tggtgccaac gcgtggagcg attgtcagag cgcgttttga tacccgcgtt
     3781 ggttaccgcg tattaatgac gctgcttcgt tccgcgggca gcccggtgcc ctttggagca
     3841 acggcaacgc taatcaccga taaacaaaac gaggtgagca gtatcgttgg tgaagaagga
     3901 cagctctata ttagcggaat gccagaggaa ggacgggtat tgattaaatg gggtaatgac
     3961 gcgtcgcagc aatgcgtggc gccttataaa ttatccctgg aattaaaaca gggcggaatt
     4021 attcctgttt cggccaattg ccagtaagca aggagagcga aatgaaagcg gcaatagcat
     4081 tatccttatt aggatgcgta ttcggatttt ctggcaaggc atttgccgga gatgcgtggg
     4141 ggccatgtac ccccgcggat ggcacgactt atcactataa tgtcgatgtg gatgttggta
     4201 tacctgatgc ggcaaaaaac gtcgcaggaa cggttttacc ggatgtcctt aactggtcta
     4261 atggacaaaa cgtatcgcta atttgtgaat gtcctgattc ttataagaat gaaaaagata
     4321 ctctagtgca gggggtgagt atgcttcccc cttctggccg tacggttgac agtatgaaat
     4381 attatacttt gacggaggaa ctggaggttg cgacgaacat tcgcatcagt accagtgtgt
     4441 atgggtttgt tcctttcaaa aaccagcagg cgctgcaaac aaccggatgc aacaaagtca
     4501 ttactacgcc ctatatgggc ggcgcaggtc tactttcctt tgctattact aaacccttta
     4561 tcggcgattc cgtcatccct ctgacgttaa ttgccgaact gtatgcctcg aaaacaaata
     4621 aagattacgg aacaataccc atatcttccg tatctattca ggggcgtgtc accgtaaccc
     4681 aggattgcga aattaaaccc ggcacggtgc tggatgtgcc atttggtgaa ttcccctcct
     4741 cggcgtttaa aaacaggcaa gggcaaatgc ctgaaggtgc gacagagcaa gaaattaacc
     4801 tcagcttcga ttgcaacaat atctctgatg gtatcaaggt tgcgttgcgc cttgaggggg
     4861 caaccaatgc tgacgatccc cgagcggtgg atatgggcaa ccccgatatt ggcgtactgg
     4921 tgaaagattc cagcggcaaa attttggtac cgaatgattc aagcagcacc acgttattaa
     4981 atttgagcag cctggattct aaaactcacc gcaatgccgc gattcgactg ctggctttac
     5041 cgattagcac gacgggtaaa gcgcctaaag gcgggacgtt tgaaggcgta acgaccatat
     5101 atcttgaaat ggagtgatgc catgaagttc aaacgattgc tgcatagcgg catcgccagt
     5161 ttgagtctgg ttgcctgcgg ggtgaatgcg gcgacggatc ttggcccggc aggggatatt
     5221 catttctcca tcactatcac cactaaagct tgcgagatgg aaaaaagcga tctcgaagtc
     5281 gatatgggaa caatgacgct gcaaaaacct gcggcagtcg gtacggtgtt gagcaagaaa
     5341 gatttcacca ttgaactcaa agagtgcgat gggatatcca aagcgaccgt tgagatggac
     5401 agtcagtcgg acagcgatga tgattccatg tttgcccttg aggctggtgg cgcaacgggt
     5461 gttgcgttga agatagagga cgataaagga acgcagcaag ttcccaaagg ctccagcgga
     5521 acgccgattg aatgggcgat tgatggcgaa accacgtcgc ttcactacca ggcgagttat
     5581 gtggtcgtca acactcaggc cactggtggc acagcgaatg cccttgtcaa tttttccatc
     5641 acctatgagt aagttactgg gccagcatcc tggcccaacc tatctctgaa tagtcgttaa
     5701 tccgttaaaa tggtctattc cggacttgca cttttgtaaa tttgtgcttc atgcacactc
     5761 tttccccaca ctttttccct ttgctgtggt ctacttattc gcgcgtgtag attttactta
     5821 tctgactacc tccgcacttt tcccctgctg ggcctgaaaa gccgctaagc agggtgttat
     5881 cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc
     5941 tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttaccgcc
     6001 gcttcggcag ctatgcgcac gattttaccg tctggaaagg catttctgct gttgttgaac
     6061 tggccgccac cgtactggtg accttctttt tactacgtct tctttcgctg tttggccgcc
     6121 gcagctggcg tattctggca tcgctggttg tgctcttttc cgcaggtgcc agctattaca
     6181 tgaccttcct taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata
     6241 tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagcg
     6301 cattgcctct tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc
     6361 gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt actggcggga attatggttt
     6421 gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg
     6481 atttgccgag ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg
     6541 cgttggggct gtacgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta
     6601 acccggcgaa gaaattcact tatcaggcac cgcaaaatgt taatgatact tatgtcgtgt
     6661 ttatcatcgg tgaaaccacg cgttgggacc atatgggcat tttcggctat gagcgtaata
     6721 ccacgccgaa gctggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata
     6781 ccgcaaccaa actctctctg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc
     6841 cgcagcgcac attaaaagaa cagaacattt tcgcggtact gaagcagtta ggattcagtt
     6901 ctgacctcta cgctatgcag agcgaaatgt ggttctacag caatacgatg gcggacaata
     6961 ttgcttaccg tgaacagatt ggtgcggagc cacgtaaccg tggcaagccg gtagatgata
     7021 tgttgctggt agacgaaatg cagcaatcgc tggggcgcaa cccggatggt aagcatctaa
     7081 tcattctgca taccaaaggt tcgcacttta actacaccca gcgttacccg cgcagctttg
     7141 cgcagtggaa gccggaatgt attggtgttg atagcggatg taccaaagcg cagatgatca
     7201 actcctatga caactcggtg acctatgtgg atcactttat ctccagtgtg attgatcagg
     7261 ttcgcgataa gaaagcgatt gtgttctacg cagctgacca cggcgagtca attaatgaac
     7321 gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga
     7381 tgatggtctg gatgtcagat aaatatctgg aaaatccggt caatgcgcag gcgtttgcgc
     7441 agctgaaaaa agcagccgac atgaaagtgc cacgccgtca cgtagagctg tacgacacca
     7501 tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt
     7561 gtcacatccc gcagacaaaa gaggcagcgg ctaactaaga ggcctgctga ctttctcgcc
     7621 gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg
     7681 ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
     7741 aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     7801 tgcatctgca gcccatgagg aagagaacct ccgggggcag ggaggttcga ctcgacgtag
     7861 gcctgataag acgcgtcagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg
     7921 gcgtgaacgc cttattcgac ctacacgcca cacatttcc
//
LOCUS       DBFIFS010000202         7905 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.202, whole genome shotgun sequence.
ACCESSION   DBFIFS010000202 DBFIFS010000000
VERSION     DBFIFS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.202"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            100..189
                     /gene="kdpF"
                     /locus_tag="N7A07_003898"
     CDS             100..189
                     /gene="kdpF"
                     /locus_tag="N7A07_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU2478472.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            189..1862
                     /gene="kdpA"
                     /locus_tag="N7A07_003899"
     CDS             189..1862
                     /gene="kdpA"
                     /locus_tag="N7A07_003899"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU2478473.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1885..3933
                     /gene="kdpB"
                     /locus_tag="N7A07_003900"
     CDS             1885..3933
                     /gene="kdpB"
                     /locus_tag="N7A07_003900"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU2478474.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3942..4514
                     /gene="kdpC"
                     /locus_tag="N7A07_003901"
     CDS             3942..4514
                     /gene="kdpC"
                     /locus_tag="N7A07_003901"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU2478475.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4507..7191
                     /gene="kdpD"
                     /locus_tag="N7A07_003902"
     CDS             4507..7191
                     /gene="kdpD"
                     /locus_tag="N7A07_003902"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU2478476.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7188..7865
                     /gene="kdpE"
                     /locus_tag="N7A07_003903"
     CDS             7188..7865
                     /gene="kdpE"
                     /locus_tag="N7A07_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU2478477.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1621 a         2072 c         2351 g         1861 t
ORIGIN      
        1 tttatacttt ttttacaccc cgtccgcaga tttttgcgaa atctttgcag ccagaattct
       61 acccttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      121 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat ccatgcggag
      181 gcattctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      241 tgctggcgcg tcccttaggt agcgggctag cgtggctgat taatgatatt cctcttcctg
      301 gtacgacggg cgttgaacgc gtactcttta gcgcgcttgg cgtctctaac catgagatga
      361 actggaagca gtatctctgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
      421 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
      481 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
      541 atagcggtga aaccacgttg agctatttca gccagatggc gggcttaacg gtgcaaaact
      601 ttctttctgc cgccagcggg attgcggtga tttttgccct cattcgtgca tttacccgcc
      661 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
      721 tcgtccctgt ggcgttggtg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
      781 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta ccaatgggtc
      841 cggtggcttc tcaggaagcg atcaagatgc tcggcactaa cggcggtggt ttctttaatg
      901 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
      961 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1021 gtcaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1081 tgatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1141 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1201 tgactacggc ggcgtcctgt ggcgcggtga ttgcaatgca tgattcgttt accgctctcg
     1261 gtggcatggt gccgatgtgg ctgatgcaaa ttggtgaagt ggtattcggc ggtgtcggtt
     1321 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     1381 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgtgagatg aaactgactg
     1441 cactggcgat tctggtcacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga
     1501 ccgacgccgg acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt
     1561 acgccgtgtc atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     1621 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     1681 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     1741 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     1801 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     1861 gatgatattg agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     1921 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     1981 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2041 atggcaagcg gcgcgatgcc tggcaatgcg ctgtttagcg tggccattag cggctggctg
     2101 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2161 caggccaaca gcctgaaagg ggtgaaaaaa acggcctttg cccgcaagct gcgtgagccg
     2221 aaatatggcg ctgcggcgga caaagttccc gccgatcaac tgcgtaaagg cgatatcgta
     2281 ctggtagaag caggcgatat tatcccctgc gatggtgaag ttattgaagg cggcgcatcg
     2341 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     2401 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     2461 gttaaccccg gcgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     2521 cgcaaaacgc cgaacgagat tgccctgacc atcctgctga ttgccctgac tatcgtcttt
     2581 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc agtcagcgta
     2641 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     2701 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtggttgc caccagcgga
     2761 cgtgcagttg aagcggcagg tgacgttgac gttctgctgc tggataaaac cggcaccatc
     2821 acactcggta accgtcaggc atcggagttt atccctgcgc agggcgtgga tgaaaaaacg
     2881 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt
     2941 gtgatcctcg ccaagcagcg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3001 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaatatcga caaccgcatg
     3061 atccgtaaag gttctgtcga tgccattcgc cgccatgttg aggctaacgg tggtcacttc
     3121 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggggccac accgctggta
     3181 gtagtagaag gttctcgtgt gctgggcgtt attgcgttga aagatatcgt caaaggcggc
     3241 attaaagaac gcttcgccca actgcgcaaa atgggtatta aaacggtgat gattaccggc
     3301 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gcgtcgatga ttttctcgcc
     3361 gaagcgacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggccgtttg
     3421 gtagcaatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     3481 gtggcgatga actccggtac tcaggcggcg aaagaagcgg gcaatatggt ggatctcgac
     3541 tctaacccga ccaagttgat cgaggtggta catattggca aacagatgct gatgacccgt
     3601 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     3661 gcggcattcg cggtaacgta tccacagtta aatgcgctga acatcatgcg cctgcattcg
     3721 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     3781 cccctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     3841 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     3901 gatttactac tgaccgtttg cggtctggtg tgaggtttac catgcgtgga ttacgtccgg
     3961 cattatcgac atttatcttt ctgttattga ttactggcgg tgtttacccg ctgctgacca
     4021 ccgcgctggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4081 atacggtgcg gggttcggca ttaatcgggc agaattttac cgacaacggc tattttcatg
     4141 gtcgcccgtc ggcaacggca gaaatgcctt ataatccaca ggcttctggc gggagcaatc
     4201 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4261 ccgctaaccc gaatgccagc acgagcgttc cggttgaact ggtgacggcg tcggcaagcg
     4321 ggctggacaa taacatcacc ccgcaagcgg cggcctggca gatcccacgc gtagcgaaag
     4381 cgcgtaatct cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccac
     4441 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcactggata
     4501 aacttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     4561 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gcgcaggcgt cgggaagacc
     4621 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     4681 gtggtagaaa cccacgggcg gaaagatacc gccgccatgc tggaagggct ggctgttctg
     4741 ccgccaaaac gcctggcgta ccgtgggcgg cacatcagcg agtttgatct cgatgccgcc
     4801 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     4861 tctcgtcatc ctaaacgctg gcaggatatc gaagaactgc tggaagctgg cattgatgtt
     4921 ttcacaaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     4981 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtg
     5041 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5101 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5161 cgcaaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5221 gggcatcctg gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     5281 cataacaccg gcagcgaaaa actggtccgc gcggcggcgc ggctggcgtc acggctgggg
     5341 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     5401 cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca
     5461 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     5521 aagattattc tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     5581 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     5641 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     5701 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     5761 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctacttgg cgtggtggtg
     5821 gtggcgctat tttatggacg ctggccttcg gtggttgcta ccgtcattaa tgtggtgagt
     5881 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     5941 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgcgggtgtg
     6001 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6061 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6121 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6181 cagccgttaa cacatccgca aggaatgacg ccgtgggacg atgccatcgc gcagtggagt
     6241 tttgataaag gcctgcctgc gggtgcgggc accgacacgt tacccggcgt gccgtaccag
     6301 attttgccgt taaaaagcgg tgagaaaacc tacgggctgg tggtggtgga accggggaat
     6361 ctgcgtcagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     6421 gccaatgcgc ttgagcggct gacgctgacc gccagcgaag aacaggcgcg gatggcaagc
     6481 gaacgtgaac agatccgcaa cgccctgctg gcggcgcttt cgcatgattt acgcactccg
     6541 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     6601 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     6661 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     6721 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     6781 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     6841 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     6901 attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     6961 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7021 gagtcggcag taccgggggt agggcttgga ctggcaattt gtcgggcgat agtggatgta
     7081 cacgggggca ctattaccgc gttcaaccgg ccggaaggtg gtgcctgttt tcgtgttaca
     7141 cttccccagc aaactgcccc tgaacttgaa gaatttcatg aggatatgtg acaaacgttc
     7201 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7261 ggatgcgcgt ctatgaggcc gaaacgctgc aacgcggctt gctggaagcg gctacccgta
     7321 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     7381 gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     7441 gcgacaaaat cgccgcgctg gatgccggag cggatgatta tctaagtaag ccgtttggca
     7501 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     7561 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     7621 ggggtgagga agaggtgcat ctcaccccga ttgagtttcg cctgctggca gtgctgctca
     7681 acaacgccgg aaaagtgctc acccagcgcc aactccttaa ccaggtgtgg gggccaaacg
     7741 cggtcgaaca cagtcattat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     7801 aggatcccgc tcgccccagc catttcatta ctgaaaccgg tattggttat cggtttatgc
     7861 tttgaatttt tattcaataa attcgcctaa atagtgatta attta
//
LOCUS       DBFIFS010000203         7882 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.203, whole genome shotgun sequence.
ACCESSION   DBFIFS010000203 DBFIFS010000000
VERSION     DBFIFS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.203"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            593..1042
                     /gene="alaE"
                     /locus_tag="N7A07_003904"
     CDS             593..1042
                     /gene="alaE"
                     /locus_tag="N7A07_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU2478478.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(1079..1423)
                     /locus_tag="N7A07_003905"
     CDS             complement(1079..1423)
                     /locus_tag="N7A07_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU2478479.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1575..1904
                     /locus_tag="N7A07_003906"
     CDS             1575..1904
                     /locus_tag="N7A07_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU2478480.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(2054..3388)
                     /locus_tag="N7A07_003907"
     CDS             complement(2054..3388)
                     /locus_tag="N7A07_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2478481.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3476..3907
                     /locus_tag="N7A07_003908"
     CDS             3476..3907
                     /locus_tag="N7A07_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU2478482.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            4116..4361
                     /gene="nrdH"
                     /locus_tag="N7A07_003909"
     CDS             4116..4361
                     /gene="nrdH"
                     /locus_tag="N7A07_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU2478483.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4358..4768
                     /gene="nrdI"
                     /locus_tag="N7A07_003910"
     CDS             4358..4768
                     /gene="nrdI"
                     /locus_tag="N7A07_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU2478484.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4741..6885
                     /gene="nrdE"
                     /locus_tag="N7A07_003911"
     CDS             4741..6885
                     /gene="nrdE"
                     /locus_tag="N7A07_003911"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU2478485.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6895..7854
                     /gene="nrdF"
                     /locus_tag="N7A07_003912"
     CDS             6895..7854
                     /gene="nrdF"
                     /locus_tag="N7A07_003912"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU2478486.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
BASE COUNT         2000 a         2004 c         1930 g         1948 t
ORIGIN      
        1 cacaaaattt aatctattta cagtcctgcg cgagattatt tcagctgtta ttttctgaaa
       61 ctctcagaat aatttcaaga ttatatatta gccattttga tgagtaattt agattattgg
      121 tgaacagaac ggcgctctct gctcgctaat tccgcaagcc tgacaaaacc aaagtgaata
      181 aaaatgcaat ataacagaat aaaataaaac acccctcact ggtgctttct gctgttaatg
      241 tacattttct ggcataaaaa atgctgtaaa tgacccggtc acgacataca ccgaattctc
      301 tacgcttata gcgttacctc acccccaaac tttcattagt gaaattaaag gaaaggtcat
      361 atccaattga tttaattgaa aataaatcat atcggcatgt aaaaatccac aatgcacaaa
      421 aaacgagccg ttacggaata ttttatctac aaaaactgac taaataaaaa tttttcacta
      481 attgattagt catagccagc gatatacgct atgcgaaaat gcagatggca atgagatcca
      541 ctgctttcat ctccattaac atcccattac gcttttatta aggagcatta gcatgttctc
      601 accgcagtca cgcttgcgtc atgcagttgc agatacgttc gcgatggttg tttactgttc
      661 tgtcgtgaac atgtgtattg aagttttcct ctccggaatg agcttcgaac agtcttttta
      721 ttccagattg gtagcgattc cggtgaacat cttaattgca tggccatacg gtatgtaccg
      781 cgatctgttt atgcgcgcgg cacgcaaagt tagcccgtcg ggctggataa aaaatctggc
      841 ggatatcctg gcttatgtga cgttccagtc accggtgtat gtggcgatct tgttagtggt
      901 gggcgcagac tggcatcaga ttatggcggc ggtcagttca aacatcgttg tttcgatgtt
      961 gatgggggcg gtttatggct acttcctcga ttattgccgc cgactgttta aagtcagccg
     1021 ttaccagcag gtaaaagcct gactcttctt ttcgcgactg gcatcgccag tcgcgtcttt
     1081 aactggcttc gccaaataaa ccattcaaat aacgttcaag cgcaacacgc gaactaaagc
     1141 catgcgggat accgtaatgc tcgtgtcgct cgcctgctaa atagagcgga aattgctggt
     1201 aatgatcgca agttttaata tcggaagcgg gttcagccag tgtcgaactg cgttctttta
     1261 atgttggatg aattaccaga gcggtacgtc ccattcgcgc ttcgcgatta acatagacat
     1321 aattttcccc acggcggtaa ccatacgctt tctgggttac cacatcgaca gtaaaaccga
     1381 ctttttcaag tacgcgcgcc acctcgtctg gtcgtaaata catatttgat cctcgttatt
     1441 atcaaccgcg ggcctacctt acctgattgc gcattcacaa cgctttcaga aaagtccata
     1501 aagccgcgat gaacctcagt taagaaaatt atggtctaca ctgaaaatta catcgaattc
     1561 taatggggga tcatatgttt aaccgaccga accgcaacga cgttgatgat ggcgtgcagg
     1621 atatccagaa tgatgtcaat caattagctg acagcctcga atctgtattg aagtcctggg
     1681 gcagcgacgc caaaggggaa gctgaagccg cacgcagtaa agctcaggca ttgctgaaag
     1741 aaaccagggc gcgaatgcat ggtcgtactc gcgtccagca agccgcgcgc gatgccgtag
     1801 gctgcgcgga ttcttttgtt cgtgaaagac cctggtgtag tgtgggaact gctgctgcgg
     1861 taggtatttt tatcggtgcg ttgttgagca tgcgtaaatc gtagtgagac gatgacaagc
     1921 agtagtctga cttctgaaaa gtactcagtg cgtcaacgcc ttgtccgcct acaggcctga
     1981 aaaacatagc gcatcagggg attttgccct ggtcaccagt cttataggac cccgaagcct
     2041 ggcttcgggg ttattaattt aaagcactgc gcaattgctg tgctacctgt cgcgccatac
     2101 tttcgctaac gatattcgta aacgagagca ataatcctcc gtcctcgctt gagcggatac
     2161 gccagtcgct aagtgcctgt acggcaagac cagcagcatt ggctttatgc acgaatgtca
     2221 cgtcattacc tgctagtttg atcaccatct ggataccgcc tttttgcggc acaacctgaa
     2281 atccctgctg cgtaagtgcc tgctcaatcc actgccgtcg ctgggcataa tgctggcgca
     2341 ttttcttcag atgccgccag aaatgcccct cacggatgaa atctgccagt gtgttctgcc
     2401 acagtacggg tacagcacat ggttccagtg acgcctgctg ccggaattgc gaaatctgct
     2461 ccaccggcac caccagccac gcacagcgca atgcaggaaa tagcgctttg ctgaatgttc
     2521 cggcataaat cacccgctgc ggcgcgtcga gactttttag tgccggtaac ggcttaccgt
     2581 gatagcgaaa ctcactgtcg taatcatctt caataatcca tgcctgacta cgctctgccc
     2641 attccagtat ctgataccta cgcgctaaag agagtgccac acccagcggg ctttggtgtg
     2701 ccggagtaat cagggcaaaa cgcgcatcag gataattttg tattccgctt gtgacatcca
     2761 gtccgttgtc atcaaccggc acaggtaaaa tttccacacc gtggcgagtg acaatcgggc
     2821 gaatcagcgg aaagccggga tcttccatcc acatcccgtc tcccggttgc gccagagcgt
     2881 gcagaattaa agccattgag gccgcataac cgtgagtgat aaagacctgc tctggctgac
     2941 aatcaatccc acgtgaaacg cgtaaataat caacaatagc ctcgcgcaag gccgcctcgc
     3001 cgcagacatc gcctaatgcc aaatcaaagc gcgtctgggt acgcagacgg cggcccatca
     3061 cccgcgccca caattcacgc ggaaaaagat caagtgcagg tagccccatc tgaaaaggca
     3121 gcggctgctg actttcacca gcaaaagcca cgacatccac agcagactgt tgcggatgta
     3181 tctgctcact gacaaaggta cccgcctgtc cccgcctgac cagccatccc tgcgccacca
     3241 gctcagcata agcattttcg acagtggatc gagagacacc cagctcctgc gaccaggtac
     3301 ggctcgaagg tagccttgcg ccaggtttca gttttccttg ctcgataacc gtttttaact
     3361 gacgggcgat atcctgataa cgtggcatat ggtctgcttt tttaggtaat ttatggctct
     3421 ctttagcagt ccattatagt gctacattgc gggcgttaat aagaggagaa actgtatgac
     3481 tacattacgt cagccttact acgaacttag cccggcagtg tataacgcac tggtgcaggc
     3541 caaaacggca ctggaaaata gcacgctgga taccacgttg atggagttaa tttatttgcg
     3601 cgtctcgcaa atcaacggct gcgcattttg tctggagatg cacagcaaag cattgcgcaa
     3661 atccggtgtg ccacaacaca aactggacgc cctggcaggc tggcgcgtaa gccatcattt
     3721 tgatgaacgc gagcgagcgg cgctggcatg ggcggaatct gtgaccgata ttgccagaac
     3781 ccatgcggaa gacgaggttt atcagccttt gcttgagcat ttcagcgcag cagaaatcag
     3841 cgacttaacg tttgccatcg ggctgatgaa ttgttttaac cgtctggccg tttccatgcg
     3901 gatgtaattt gctgttgccg ggtatgcgcc cggcaaaaaa acacaaaatc ccctaccccg
     3961 ttgagccatt caccggtggc gtaatcaatc taaaattacc atatcttgtg tggttgaatc
     4021 ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac gcgtatcatc
     4081 gcgccgtatc ttcattttaa acggaaatac gaatcatgcg cattactatt tacactcgta
     4141 acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc tttgattttg
     4201 aaatgattaa tgtcgatcgc gttcctgaag cggcagaagc gttgcgtgct cagggctttc
     4261 gtcagttgcc ggtagtgatt gctggcgatc ttagctggtc tggtttccgt ccggacatga
     4321 ttaaccgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg tctacttctc
     4381 cagcagctcc gaaaacacgc agcgttttat cgaacgttta ggtctgcccg cggtgcgcat
     4441 cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga tcgtgccctc
     4501 ttacggcggc ggcggtacgg ctggcgcggt gccacgacag gtaattcgct ttttaaacga
     4561 cgagcacaac cgggcgttgc ttcgcggcgt tattgcttcg ggtaatcgca actttggtga
     4621 ggcgtatggc cgcgccggag atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg
     4681 ttttgaactc atgggtacgc aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt
     4741 ttggcaacga caaccgcaga atgcctgacg cagaaaacga tggattacca cgcgctgaat
     4801 gcgatgctta acctctacga tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc
     4861 gttgacgcct ttatggcgac gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag
     4921 cagcgcctga actggctggt caacgaaggt tactatgatg aaagcgttct taatcgctac
     4981 tctcgcgact ttgtcattac gctgtttgcc cacgcacaca ccagcggttt tcgtttccag
     5041 acattcctcg gggcatggaa gttttacacc agctatacgt tgaagacatt cgacggtaaa
     5101 cgttatctgg aagattttgc cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc
     5161 gatgagacgc tggcgttgca actgacagat gaaatgctgt caggacgctt tcagccagcc
     5221 acgccaacat tcctcaactg cggtaagcag cagcgcggcg aactggtttc ctgttttttg
     5281 ctgcgtattg aagacaatat ggagtcgatt ggtcgggcgg taaattccgc actgcaactg
     5341 tcgaaacgcg gcggcggcgt agcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca
     5401 attaaacgta ttgaaaatca atcttctggc gtaattccgg tgatgaaaat gctggaagac
     5461 gcattttcct atgccaacca actcggcgct cgtcaggggg ctggtgcagt ctatttacat
     5521 gctcatcatc ccgatattct gcgttttctc gacacgaaac gggaaaatgc cgacgaaaaa
     5581 atccgcatta aaacactgtc gcttggcgtg gtgatcccgg atatcacttt ccatctggca
     5641 aaagagaatg cgcagatggc gctgttttcg ccttatgacg tagagcgagt ttatggcaag
     5701 ccgtttgccg atgtggccat cagccaacac tatgacgaac tggttgccga tgaacgcatt
     5761 cgcaaaaaat acctcaacgc ccgtgatttc ttccagcgac tggcagaaat ccagtttgag
     5821 tccggctatc cctacatcat gtatgaagac acggtaaacc gtgctaaccc tgtcgccggg
     5881 cgcataaata tgagtaatct ctgctcagaa attttgcagg ttaacagcgc ctcagagtat
     5941 gacgagaatc tcgactatgc ccgcacaggc catgatatct cctgcaattt aggttcgttg
     6001 aatattgcgc acaccatgga ttcccccgat tttgcccgca cggtagagac tgccgtgcgc
     6061 ggtttaacgg cagtatcaga tatgagtcat atccgcagcg tgccgtccat cgaagccgga
     6121 aatgccgcct cgcacgccat cggactgggg cagatgaatt tacacggcta tctggcgcga
     6181 gaaggcatcg cttatggttc gccggaagca ctggatttca ccaatctcta tttctatgcc
     6241 atcacctggc acgcactgcg tacctcgatg ttgctggcac gcgaacgcgg tgaaaccttc
     6301 gccgggttca aacagtcacg ctatgccagt ggtgaatatt ttagccaata tctgcaaggg
     6361 aactggcagc cgaaaacggg gaaagttggc gaactgtttg cccgtagcgg tattacgtta
     6421 cctacccgtg agatgtgggc gcaactgcgc gacgacgtga tgcgctacgg catttacaac
     6481 cagaatcttc aggcggtgcc gccaacaggt tctatctctt atatcaacca tgctacgtcg
     6541 agtattcatc cgattgtggc gaaagtagag atacgcaaag agggcaaaac aggacgcgtt
     6601 tactaccctg ccccgtttat gactaacgag aatctggcgc tgtatcagga cgcttacgaa
     6661 attggcgcag aaaagatcat cgacacctac gcggaagcga ctcgccatgt cgatcagggg
     6721 ctgtcgctga cgctgttttt ccccgatacc gccaccactc gcgatatcaa caaagcgcag
     6781 atttacgcct ggcgcaaagg tatcaaaacg ctctattaca tccggctgcg tcagatggcg
     6841 ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa
     6901 ctctcacgta tcagcgccat caactggaac aagatatctg acgataaaga tctggaggtg
     6961 tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg tgccgctgtc gaacgatatt
     7021 cctgcctggc agacattaac tgtcgtagaa caacaactga cgatgcgcgt ttttactggc
     7081 ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgca
     7141 ctcacgcctc atgaagaagc ggtattatcg aatatcagct ttatggaagc ggttcatgcc
     7201 cgctcttaca gttcgatttt ctcgacgctg tgccagacca aagatgtcga tgccgcctac
     7261 gcctggagtg aagaaaacgc gccgttgcag cgaaaagcac agattattca gcaacattac
     7321 cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc
     7381 tattccggtt tctggctgcc gatgtatttt tccagccgcg gaaagctgac caataccgcg
     7441 gacctgatcc gtctgattat ccgcgatgaa gcagtccacg gttactacat aggctataaa
     7501 tatcagaaaa acatggaaaa gatatctctg ggacaacgtg aagagttgaa gagtttcgcc
     7561 ttcgatttgt tgctggaact ctacgacaac gagttgcaat acaccgatga gctgtacgcc
     7621 gaaaccccgt gggctgacga tgtgaaagcg tttctctgtt acaacgccaa taaggctttg
     7681 atgaatctgg gctacgaacc gttatttccc gcagaaatgg cggaagtgaa tccggcaatc
     7741 ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc
     7801 tcttatgtga tggggaaagc ggttgaaaca gaatatgaag actggaattt ctgagggtgt
     7861 tattttcaaa aatatcacta cc
//
LOCUS       DBFIFS010000204         7850 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.204, whole genome shotgun sequence.
ACCESSION   DBFIFS010000204 DBFIFS010000000
VERSION     DBFIFS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.204"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(15..986)
                     /gene="ftrA"
                     /locus_tag="N7A07_003913"
     CDS             complement(15..986)
                     /gene="ftrA"
                     /locus_tag="N7A07_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HGU2478487.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            1081..1476
                     /locus_tag="N7A07_003914"
     CDS             1081..1476
                     /locus_tag="N7A07_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU2478488.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            complement(1517..2704)
                     /gene="ydeE"
                     /locus_tag="N7A07_003915"
     CDS             complement(1517..2704)
                     /gene="ydeE"
                     /locus_tag="N7A07_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU2478489.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            2899..3798
                     /gene="eamA"
                     /locus_tag="N7A07_003916"
     CDS             2899..3798
                     /gene="eamA"
                     /locus_tag="N7A07_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU2478490.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            complement(3829..4047)
                     /gene="marB"
                     /locus_tag="N7A07_003917"
     CDS             complement(3829..4047)
                     /gene="marB"
                     /locus_tag="N7A07_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU2478491.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4079..4462)
                     /gene="marA"
                     /locus_tag="N7A07_003918"
     CDS             complement(4079..4462)
                     /gene="marA"
                     /locus_tag="N7A07_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU2478492.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(4482..4916)
                     /gene="marR"
                     /locus_tag="N7A07_003919"
     CDS             complement(4482..4916)
                     /gene="marR"
                     /locus_tag="N7A07_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU2478493.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            5128..5793
                     /gene="marC"
                     /locus_tag="N7A07_003920"
     CDS             5128..5793
                     /gene="marC"
                     /locus_tag="N7A07_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU2478494.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            complement(5818..7008)
                     /gene="ydeA"
                     /locus_tag="N7A07_003921"
     CDS             complement(5818..7008)
                     /gene="ydeA"
                     /locus_tag="N7A07_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU2478495.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHLSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7159..7850)
                     /gene="yneK"
                     /locus_tag="N7A07_003922"
                     /pseudo
     CDS             complement(7159..7850)
                     /gene="yneK"
                     /locus_tag="N7A07_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2062 a         1817 c         1990 g         1981 t
ORIGIN      
        1 gctgaatcag gcgattattg tgatggggaa cggctgtttt tgcgatattg cgaaggagat
       61 aataaaaaac gttggtgaaa gtgatgacgc aataacgctg aattgccaaa acccgtcagc
      121 tcggcaattt tctcgatgtt aaaaaccgaa ttttgcagat aatcctgtac tcgtattaag
      181 cgctcattga gcagccagcg ggcgggcgtc atgccagtta acgcctcaaa acgtcttaaa
      241 aaagtgcgcg gactcatatt tacccgttcg gcgagggaag acacagtatg tgtttgtgcc
      301 agattctgct gcaaaaagtc gaataaggca cctaacgtct ggctttcacg cggtgaggcc
      361 attgggcgcg tcagattttg tggttgatcc ccctggcgat gtggctgaat gaccagtcgc
      421 cgggcgacat tattagcaat ctcatgacca aaatctttgc gcacgatatg taaacacaga
      481 tcgatccccg cggcgcttcc ggcggaggtc atcacttgtc cttcatcctg atataacaca
      541 ttgtcactta cgctgattga agggtaacgg tgctggagtt gttcagtgta gcgccagtgg
      601 gtggttgctt ttcgctgatg aagaaggccc gcagcagcaa ggacaaacac gccagagcat
      661 attgatacca ggcgagtacc gcgctggtgt gcctcgcgca atgactggca tagcatctcc
      721 ggcactttgg catctattcc gcgccagcca ggaataataa tcatatcggc gttgggcaat
      781 aactccagtc caccatcagc aaccacgtgg atccccccgg tggcttgtag cgcacctggt
      841 tctaccgcag caacagcaaa acgataccag ttatcgccca gttccggtcg tggcaggccg
      901 aatatttcaa cggcaacgcc aaattcaaaa gtacatagcc cgtcatatgc cagtgcaatg
      961 accagcggac gttgtaacgt tgtcatcatt ttgctcattt ctggcatatc gagaggcggt
     1021 cagtgaaagg tagattagtt acattattgt taacacaaca aaccaaaagg acaaagcaat
     1081 atgcgttatg tcactgaatt taccacagcg aagccagaag ttgctgaggc gcattttctc
     1141 gcgcgcttga gtgtcgaaac agattgcgcc gatgttcatg actctctggc ttatattggc
     1201 gatgatttcg ttttacttca tgtggttgga cgtgaagagg acttcgcgcg tcggcatatc
     1261 ccaggcgctc tccatttacc ccataagcaa atgaccgcag aaagaatggc cgaatggccc
     1321 gacgatacgc tatttgtggt gtattgcgcg gggccacatt gtaacggtgc agaccgtgcg
     1381 gcactcaaac tggctcgact cggaaagccg gttaaaatca tgctcggcgg gataaccggc
     1441 tgggaagatg aaggctatgc ctttgcctca gaataatgat tagcgaagcc gtgccagtag
     1501 tggaacggca cgaacgtcaa cagagtgctg gttgcctctg gggtctggcg ttaatacctt
     1561 ttaacatcag cgcccacgcg gcaatgatga ctaacgccag aatgataaac agcgaagacg
     1621 acgggagagt ggtaagtaca acaccactca ctaatggatt taccgctgcg cccaaccagc
     1681 ctaatgactg ggcggaaaaa tagctggctt tcattcctgg cggcgcaata tggtcaatca
     1741 acatatactc gcccggcgca taaattattt caccgacagt aaataccgca gctgacatac
     1801 cccacaatag caggctgttg ccggaaaaaa taaaaccgat cagaccgatg acgaaacaga
     1861 gggtgcctgc tgtcatcagc gcgcggatgt tagccgggtt aaggcggcgg cccacggaat
     1921 attgcaacgt aaccaccatg gcagcattca ctggaagaac aaccgcgacc accttttcgg
     1981 caaaatcacc atcagcaatc accatcacat attgtgaaat gcatgaagca aatgcgccac
     2041 taacaaaaga agccagaaag ccagagcagg taaaccacaa cagtgcctta tcttgtaata
     2101 aaactttcgg cgaccagaca ctgcctgttt ccgtggcgat gattttctcg ctgcgcttta
     2161 cccaaatttg aatgaaaaac atgggaaacg cagagcagat agcggccagc caaaagggca
     2221 gattgatgct ctgcatgacc aacagcgtgc cgagtggcgg gccgacggtc cagccaatat
     2281 tgagcatggt gtagttgatc gagaagatct tcgttttgct ggtggatgaa agattgtcgg
     2341 caaaccaggc tttcagcacg gtagcaaaaa cggaataggc gcagttaatg agggcaaaaa
     2401 agagcaccac cagcgtcacg ttattcacta aaggaatggc aataaaaccg ctggcgaagg
     2461 cggtaattgc cagtaacata tagcgtttct tgtcgaactt atccgccagg ataccaaaac
     2521 cgaggctaaa aacgacgcca atagtgagcg caattgtcat cgcataaccg attagatcga
     2581 cactcaggct gtactggcga ctcaagtaaa tggtcataaa tggcagcgtg gcgccgcgtc
     2641 cgatggttaa taacaacgac gaggcaagaa gggcgctggt agagcgtcgt agggataagt
     2701 tcatttgtct gtccggcagt gcgtttcgtt atgttttgtt ttagagatgt aaagaaatag
     2761 catggcaaaa atccgaagta tagcgactta tttgcgcatt aatgccgtcg taaccggcga
     2821 ctaacgaatc aactgttaaa gaaaagtaaa ttcagctaag ttgattgctt acaaaagatg
     2881 aaattcagag gcggtagaat gtcgcgaaaa gatggggtgt tggcgctact agtcgtcgtt
     2941 gtatgggggc ttaattttgt ggtcatcaaa gtggggcttc ataacatgcc accgctgatg
     3001 ctggccggtt tgcgctttat gctggtcgcc ttcccggcaa tctttttcgt ggcacgacca
     3061 aaagtaccgc tgaatttact gctggggtat ggtttaacca tcagttttgc acagtttgcc
     3121 tttctcttct gcgccattaa cttcggtatg ccggctggac tggcctcgct ggtcttacag
     3181 gctcaggcgt tttttactat cgtgcttggc gcgtttactt ttggcgaacg gctgcacggt
     3241 aagcaactgg cggggattgc gttagcgatt tctggcgtac tggtgttaat cgaagatagc
     3301 tttaacggtc agcatgtggc gatgctcggc tttatgttga ctctggcagc ggcatttagt
     3361 tgggcgagtg gcaatatctt caacaaaaag atcatgtctc attcatcccg tccggcggta
     3421 atgtctctgg tcgtctggag cgcattaatc ccgataattc cgttttttgt cgcctcgctc
     3481 attctcgatg gctccggaaa aatgattcac agtctggtca ctatcgacat gaccaccatt
     3541 ctggcgttga tgtatctggc atttgtagcg acgatagttg gctacgggat ttggggtacg
     3601 ctgcttggac gctatgaaac atggcgtgtc gcaccattat cattactggt gccggtagtc
     3661 ggtctggcaa gtgcagcgtt actgctggat gaacgcttaa caggtctgca attcttaggg
     3721 gctgtgctca ttatggccgg actgtatatc aatgtatttg gtttgcagtg gcgtaaagcg
     3781 gtgaaggtaa gaggttaaca agccccgata tgccggggcc tgaatatact acatggcttg
     3841 ttggttataa tagggcacgc ccagcgcatc cgatttgtca ctgcctgtac ccatgtgatt
     3901 taaatcgaac ggtggttgtt cctgcgatgg gggaacaacc accacatcgc cacaggaagt
     3961 aacgagcggt tgctgggttt gttccgcaac gccctgcgcg gaaaagagaa taagcgcagc
     4021 tgctattgcg gataaaagcg gtttcataat tgcctcagtg acgttgtcac gttttcaact
     4081 agctgttgta atggtttaat ggatgtaaaa agcgcgattc gccctgcata ttggtcatcc
     4141 ggtatttatg cggcggaaca tcaaagtaat ttttgaaggt tcgggtcaga gtttgttgcg
     4201 actcgaagcc atatcgttct gccagataga gtatcggctc gttactttcc ttcagctttt
     4261 gcgcgatttc cgtcatctta cggctgcgga tgtattggcc taatgaatga ccggtttctt
     4321 ttttaaacat ccgttgcagg tgccatttag agtaacccga acgctctgac actttctcca
     4381 gtgacagtgg tgattccagg ttgtcctcga tccagtccaa aatgctatga atggtaatag
     4441 cgtcagtatt gcgtctggac atcgtcatac ctcttttttg tttacggcag gactttctta
     4501 agcaaatgct caagtgttgc cacttcgtcc gccgtcaggt tttttgttaa ttcttgatgc
     4561 aggtcctggc caactaattg atggcattgt tcacatattg ccgcgccgct ggtggtaagt
     4621 tttaccagta cgccgcgctt atcattcggg ttcggcaacc tttctaccca gcctttacag
     4681 accaggcgat ccagcatacg ggtcagtgct cccaggtcga ccgacaacac ttttttcagt
     4741 tcaaccggag taatacacgc cgcgcagcgg atagagcaga gcaccttaaa ctgtgccgcg
     4801 gtaatatcca gcggagacag atactcgtta agcagacgat ctttcttctg attaaccata
     4861 tggattaagc gacccaatgg aataatttca ttgaacagat cgctggtact tttcacatta
     4921 gttgccctgg caagtaatta gttgcagggg ataatattgc ccaggcaagt ataagtcaaa
     4981 tgaatgaatt gaccgatgcc acgttttgct aaatcggttc aagttttggt caatgcgatg
     5041 caaggttagc taaagagtga cactttatcc ttgtttaccc cttatacttt tcgctgataa
     5101 cccagataca caggataaca accaccaatg ttagatttgt ttaaagcaat tggcctgggg
     5161 ctggtggtgt tactgccgtt agctaaccca ttaacaactg tcgcgttatt tcttggcctg
     5221 gcaggcaaca tgaacggtgc cgaacgtaat cgtcagtcgt tgatggcctc ggtgtacgtc
     5281 tttgccatca tgatggtggc gtattacgcc gggcaactgg tgatggatac atttggcatt
     5341 tcgattcccg gtctgcgaat tgcaggcggc ttaatcgtcg cgtttatcgg ttttcggatg
     5401 ctttttccgc aacagaaagc gattgattca ccggaggcga aaagcaagtc ggaagagctg
     5461 gaagatgaac ccagcgccaa tattgctttt gtaccactgg caatgccaag taccgccggt
     5521 ccgggaacca ttgcgatgat tattagctcg gcgtccacag tgcgtcagag ttcaaccttc
     5581 gctgattggg tactgatggt tgcgccgccg ctgatctttt tcctggtggc ggttattttg
     5641 tggggaagtc tacgcagctc tggcgcaatt atgcggctgg tgggcaaggg tggcattgaa
     5701 gcgatctccc gcttgatggg gttcctgctg gtatgtatgg gtgtacagtt tattattaac
     5761 ggcatcctgg aaatcattaa aacgaatcat taaaaaaggc ccgaatgggc ctttcaacta
     5821 ttgcgtctgt tcttcgagtg tcactggcca gcggcgaaat ataatgattg accatattaa
     5881 cgcggcaaaa gcaggcaccg tgcccacata accaatcatc gacattgaca agtgcagact
     5941 cacctgatta cctaccaacg cacccgcccc gattccaata ttaaatatcc cggagaatag
     6001 cgccatcgcg acgtcggtgg catctggtgc cagcgccagc actttaacct gcataccaag
     6061 cccgatgatc atcatcgcga tcccccagaa aatactcagc accccgaggt gtatttcact
     6121 gttcgccgca ggtagcagca gtgccaggca caccagcaac agcgcaatcg ccgtacttac
     6181 caacgcagac gcatattgat tacccagttt accgaaaatc acgctgccaa taatgcccgc
     6241 accaccgagt aataacagta atgccgtggc aaagttggcg ctgaatcccg caatgttttg
     6301 cacaaaaggc tcaatatagc tgtatgccgt gtaatgggcg gtgacaacca ccacagttaa
     6361 caaataaatg ctcatcaatg ccgggcggcg gaataatagc gggaggcttt tcaatgaacc
     6421 ggaatgctca ctgggcagta agggaagtaa cttaatcagg cacaaaaggg taataagcgc
     6481 cccgatacca atcgcgaaga aggtcattcg ccaaccgaaa tactgcccca caatgcgccc
     6541 gagaggtaat cctaatacca ttgccagtgc tgtacctgtg gcaattaaac tcaatgcctg
     6601 tgctcgcttc ccggccggag ccatacggat cgccagggac gccgtaatcg accagaaaat
     6661 cgcatgtgca aaagccacac caatgcgact gatcaccaga acggtaaagc tccacgataa
     6721 aaacgacagt acgtggctgg caataaacac cacaaacagg cagatcagta atttgcgccg
     6781 ttcaacctgg ctggtcatta acataaaagg caatgacatt agcgctacta cccatgcgta
     6841 aatggtcaac atgatgccga cctgagcggt ttgcatgtga aaactttgcg caatgtcaga
     6901 gagcaggcca acagggacaa attcggtggt gttgaagatg aaggcggcga ctgccagcgt
     6961 aacgacccgt agccacgcca ctttgcggga aacagtgttt gttgtcataa cagattatcg
     7021 ggtgagttgc gaaaaagtga tgaaatgatc ttaaaacggt taatgatgtg aatacaatgc
     7081 atttgtgatc tggatcgcgt tttccgtttc tctgacgctg attctttcac gcgtcagaga
     7141 gatggtaaac atcacggttt aatgacgatc aggaatgtct ggcgttttgg aggttgcggg
     7201 tataggcggt ctgacagggg agcgaggcct gtttttcacc agatcttcat gagtaacttt
     7261 tggcggtgtt tcatttttta caaacagttt gagggtctta cgcgccatat gtttgataat
     7321 tttatcatca cttaatgatg ctggcatcaa cacgtcattc aaatcacttt cattgggatt
     7381 cataaattgg tgatagtgta acaacatatt ggcgaaaaat aaaaacaagt cctgaaaatg
     7441 aggagattct tgttctgcta aaagggataa aaaacagatg cagacttagg gtagtacatg
     7501 tttgttagaa cctaatgaat ccatcaggct ttgcaataaa tcctctttta attcgctgtc
     7561 cgttatgtcg agaatggcgg tgatcaagtt aggatcttta ttgacgaacc atctgtgaac
     7621 ctcagagtta ttccataatc gggggagtat gacaaaatta ccgcgagaga taaaattcag
     7681 gacaagcaaa tcaaactggc gtaagtaatc tgcagcggta tggtgatccg gaggcagggg
     7741 ttcaatatgt ggtgtatgcg ctcttttgat gggaacgcca gggttgatca ttgggaattg
     7801 ttcaataaaa tcattcagaa acagaaagag cacacgctcg acgccggtct
//
LOCUS       DBFIFS010000205         7799 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.205, whole genome shotgun sequence.
ACCESSION   DBFIFS010000205 DBFIFS010000000
VERSION     DBFIFS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.205"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            71..655
                     /locus_tag="N7A07_003923"
     CDS             71..655
                     /locus_tag="N7A07_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU2478496.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(680..772)
                     /locus_tag="N7A07_003924"
                     /pseudo
     CDS             complement(680..772)
                     /locus_tag="N7A07_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            773..1519
                     /locus_tag="N7A07_003925"
                     /pseudo
     CDS             773..1519
                     /locus_tag="N7A07_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1891..2157)
                     /gene="minE"
                     /locus_tag="N7A07_003926"
     CDS             complement(1891..2157)
                     /gene="minE"
                     /locus_tag="N7A07_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU2478497.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2161..2973)
                     /gene="minD"
                     /locus_tag="N7A07_003927"
     CDS             complement(2161..2973)
                     /gene="minD"
                     /locus_tag="N7A07_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU2478498.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2997..3692)
                     /gene="minC"
                     /locus_tag="N7A07_003928"
     CDS             complement(2997..3692)
                     /gene="minC"
                     /locus_tag="N7A07_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU2478499.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4212..4580
                     /gene="ycgJ"
                     /locus_tag="N7A07_003929"
     CDS             4212..4580
                     /gene="ycgJ"
                     /locus_tag="N7A07_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU2478500.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4683..5084)
                     /gene="pliG"
                     /locus_tag="N7A07_003930"
     CDS             complement(4683..5084)
                     /gene="pliG"
                     /locus_tag="N7A07_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU2478501.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5155..5313
                     /locus_tag="N7A07_003931"
     CDS             5155..5313
                     /locus_tag="N7A07_003931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478502.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            5325..5618
                     /locus_tag="N7A07_003932"
     CDS             5325..5618
                     /locus_tag="N7A07_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU2478503.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5690..6349
                     /gene="ycgM"
                     /locus_tag="N7A07_003933"
     CDS             5690..6349
                     /gene="ycgM"
                     /locus_tag="N7A07_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU2478504.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6441..6887
                     /gene="ycgN"
                     /locus_tag="N7A07_003934"
     CDS             6441..6887
                     /gene="ycgN"
                     /locus_tag="N7A07_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU2478505.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7094..>7799)
                     /gene="hlyE"
                     /locus_tag="N7A07_003935"
     CDS             complement(7094..>7799)
                     /gene="hlyE"
                     /locus_tag="N7A07_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGU2478506.1"
                     /translation="MDSQDKYFETTQTVYEWCGVATQLLAAYILLFDEYNEKKASAQK
                     DILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVD
                     KIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTLSNTV
                     KQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAANKMINTCNE
                     YQKRHGKKTLFEVPEV"
BASE COUNT         2029 a         1716 c         1877 g         2177 t
ORIGIN      
        1 cgtgtaaaat catttattta atcgcaattt atgtaatggc ataatcaggt cggtgaaaga
       61 ggggagaaga atggataaaa gagctaaaaa tcagatagtc gatagcgata ttgcacgttt
      121 gttactcaag ctcagaaagt ctcgaaactt gactgttaca gagcttgccc aacgatctgg
      181 tgtcagtcag gcgatgatca gcaaagttga acgaggcact tcaagcccca gtgcaacaat
      241 acttagtcgg ttagctaatg cactgaatat tacgctatca aagctttttg cagagcttga
      301 gatgcaacag aattcacttg tacttttggc tgaccagcaa caacattgga ttgatgaaga
      361 aacaggcatt acccgctggt ctttgtcccc agcgggtgca tgtccggaac taataaaagt
      421 tgaaatcccc cctatggggc aattgactat ccctgcctct gcaaatgagc atctttctgg
      481 acaaactcta tggatgttaa gcggcagcct tgattttcga gtcaatcatc agactcatca
      541 tttacaagcc ggtgattgtc ttgctcttac ctttgccagc gagtaccaaa tgagaaaccc
      601 agatagcggc cagagttgtt cttacattgt cgctttcagc caaaaaaatc catgatgtaa
      661 ttctcctaaa attatcatgt cacattgcgt tataaagatg taatagaggg tagggtctgc
      721 cttgattgtc ccgctcggaa cgtcctgaaa gtttaaagcc catatgttga taaatgcggg
      781 tgccgggttt gagcagacgc tgaccggaat gacagtgggg atcgacagcc gtaatgatat
      841 tcctgagggg attgcgacgc tgggcgcttt tatgggttat tcccattcac atatcggttt
      901 tgatcgtgga ggacatggca gtgtggacag ttattctctg ggcggctatg ccagttggga
      961 acatgaaagt ggtttctatc tggacggtgt cgtgaagctg aaccgttttg aaagtaacgt
     1021 agccggtaaa atgagcagcg gtggagccgc caatggcagt taccatagca acgggctggg
     1081 cggtcacatt gaaaccggga tgcgatttac cgatggtaac tggaacctga cgccgtatgc
     1141 ctcgttaacg gggttcaccg ctgataaccc cgaatatcat ttatccaatg gcatggaatc
     1201 gaaatcagtc gatacccgca gtatatatcg tgaactgggt gcaacgctga gttacaacat
     1261 gcgtctgggg aacggtatgg aagttgagcc gtggctgaag gcggctgtgc gcaaagaatt
     1321 tgtcgatgat aaccgggtga aagtgaatag tgacggtaat ttcgtcaatg atttgtcggg
     1381 cagacgtgga atataccagg caggtattaa agcctcattc agcagtacgt taagcgggca
     1441 tcttggggtg gggtatagca acggtgctgg tatggaatcc ccgtggaacg cggtggctgg
     1501 tgtgaactgg tcgttctgac catcaacgat taaactgcgc ttcggcgcag ttttcgttta
     1561 caggatgtta aaagagaaaa ttctggggta aaaaaagccc gccagttacg gcgggaaacc
     1621 tcatcctatg ggagaacaat gaataatgaa attgcggggt tatcatctcc cagtatatca
     1681 acactaacaa taaggttatt tactcaacca ggcataaata ttttgttttg tgcgtgggaa
     1741 cagccttaag gtgtaaaggg ggaggtggaa atagcaatga ggagtatcag caagaatact
     1801 cgccgcttta ccacaacgtg gatgagaggg atgaaaaact caaggcagag ataactctgc
     1861 cttgaagata aatgcgcttt tacagcggga ttatttcagc tcttctgctt ccggtaaggt
     1921 cacgttcagc tcaagaatag aaatatcgcc atctttttgc tcaagctgta cggttaccat
     1981 ctcaggatca atttgtacgt atttacaaat gacctcaaga atatctttac gcaactgcgg
     2041 cagataatgc ggttctgcat cgctgcgacg gcgttcagca acaataatct gcagccgttc
     2101 ttttgcaatg ttggctgtat ttttcttccg cgagagaaag aaatcgagta atgccataac
     2161 ttatcctccg aacaagcgtt tgaggaagcc tttcttctct tcttcaatga agcggaaagg
     2221 acgttcttct cccaacagac gttctacggt atctgcgtag gctttacccg catcggcgtt
     2281 aatgtcgaga atgaccggtt caccctggtt agaggcgcgc aatactgatt gatcctctgg
     2341 gatcacgccg acgagtttga tgcgcaggat ctccagcaca tcttccatgc tcagcatgtc
     2401 acctctgctt acgcggcctg ggttatagcg cgttaacagc aggtgctctt taataggctc
     2461 ttcgccattt tctgcgcggc gtgattttga cgccagaatg cctaaaatac ggtcagagtc
     2521 gcgtactgag gagacttccg ggttggtggt aataatggct tcgtctgcaa aatagagtgc
     2581 cattaacgca ccggtttcaa tccctgccgg ggagtcacaa acgataaatt caaaatccat
     2641 cgctttcaga tcatcaagaa ctttagcgac cccttcacgg gtgagggcat ctttatcgcg
     2701 tgtttgcgat gccggcagaa tatagagatt ttcagtacgc ttatctttaa ttaacgcctg
     2761 atttagcgtt gcatcgccct gaatgacgtt gacgaaatcg taaacgaccc ggcgttcaca
     2821 acccataatc aggtcgagat tacgcaggcc gatatcaaaa tctatcacga cagttttctt
     2881 tcccttctgg gccaaaccag tggcgatggc cgcgctggag gttgtcttac caacaccccc
     2941 tttgcccgaa gtaacaacaa taatgcgtgc catagaaatt ccttgttaaa aagggatcaa
     3001 tttaacggtt gaacggtcaa agcgttttcg actaactgca gtcgcgccgc tttgccataa
     3061 aattctgctg ggatttgatc actcagccag tattcacctg cgatggacac caattccgcc
     3121 atcaggttcg tacaaaatat ttgcgtttcc cggtcaccac ttgcccctgc cagcgcacga
     3181 ccgcgcatca tgccatagac atgaatgttc ccatcggcaa tcaattcggc cccagcgcta
     3241 acgtggcttg taacaatcag atcacattgt ggagcataaa tacgctgacc ggaacgcacc
     3301 ggggtatcta ttaaacgcgt ttttgtgacc ggcgttgtat tttgcgctgg agcctgcggt
     3361 gcgggagctg gacgtggcgc tttttccttt ccttccgtca ggataggcag ccccattttt
     3421 tcaatttcgg ctttaagttg cgcatctttg cagccactta cgccaataac ccgcaaaccg
     3481 gttgccgaaa ccgccttatg catcgctgac cagtttaccg ggtcttccag tgcactgacg
     3541 ttgagtacaa cgggggcatg ttttaaaaat gcgggggcct gagcgatttt gtcttccagc
     3601 gcctgatgga taaccttagg ttctgcctca tgcagatgaa ccacagataa agtgaagcta
     3661 ctgcctttaa gctcgattgg cgtgtttgac atcctggcct tactcaatta gctattaatc
     3721 atcgccagcg cgcgatgatg ttccgaagac tataaggcat gttatagtct ggattatatt
     3781 gaggcaagtc accctcccat ttattcagag taaaagtcta ttctgtgata aatggcgctg
     3841 attcatagct taaaaaatac ccttgtcaat caacccattg ccgtcgtact tttgattctt
     3901 cttatttacg cttccttttt ccgcacccta atcgtgacag catctccaga cctgtttaaa
     3961 tatccagtat tgaggcaagt gtattttttc accacgccag gctttcttaa ctttccattt
     4021 tttgcagcat cattaaagaa cagatacccg ttatcttaat atccgccaca gaattgctct
     4081 atcatagaaa ccgcagaaga cgtctgatac ttctgtcagg tcatcgtaac tcgtccaggt
     4141 cagcggtttt gattacggat tgatgatgaa atgagcataa gtgttgtacg gttggattat
     4201 caggatgtgc tatgaatatg atgcgtattt tttatatcgg attgtcaggt gtggggatga
     4261 tgttctcatc aatggcgtct ggacatgacg ctggcggact tcaatctccg gcgtgcggcg
     4321 ttgtttgcga cccgtatatt tgtgtgaact cagatggcat ttccccagag ttaacaagga
     4381 aatatctcag cgaaaaagcc gctgaaaact tacaatcatt acaaggctac gatcccagcg
     4441 aatttacatt cgctaacggt gtattttgcg atgttaaaga aaaattatgc cgtgatgatc
     4501 gttattttgg tgtagatggt aagcggagtg gaaaaatcaa tcaaaccacc acaaagatgt
     4561 tatttatgtg tcgtgagtga tgtcttgttt acattatgtt ctattcgtca ttaacctctt
     4621 gagcgacaga gaggagtggg gcgtcaacgt ttatgacggg gagactcccc gtcggttgac
     4681 atttacttaa tttgaatatc gacgttatat tttttagttt tgtttttacg ggcgtcgttt
     4741 cgggtttgca gcactcgcag ctcatatttg ccagaagcag ggagtgagta ttgtccgtga
     4801 ctatcgagtt caggggaata tctggataaa tcaacagaat catcgattcc tggcccgaag
     4861 aggtaagtat cagcgccttc attagagata ctcacatgca ctttctgacc ttttttggca
     4921 tagaaggtat atgtatcgta atcgtatccc tttatttcgc cggagtactg agcgctgcta
     4981 tggccttttc tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc
     5041 agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag tcttctgttc
     5101 agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct
     5161 ttcctgcgtt cggtaaaatc ccgtcgcggc atttttgccg gggggattac ttataaaggg
     5221 gcatggactc aacaaaccgt agaaatttac agctggctgg cttgtggcgc gggtttcatt
     5281 gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt tgtgtgattt
     5341 atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc
     5401 gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgc
     5461 tggatgggcg gaagaaactg gtcaatgccg atattgaaaa ggttaaacag gcattaaccg
     5521 agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag caacatcttt
     5581 ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc ggtggcatta
     5641 tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta tgtatcaaca
     5701 tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagttt gtgttggcag
     5761 taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt
     5821 tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc catccgattt
     5881 cggttcagtt catcatgaag tcgaactagc ggtgttgatt ggcgcgacgc tgcgtcaggc
     5941 tacggaagag catgtccgca aagccattgc cggttacggc gtggcgctcg atctgacgtt
     6001 gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt
     6061 tgataactct tgtccgcttt ccgggtttat tcccgcggcg gaattcaccg gcgatccgca
     6121 aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga
     6181 catgatccat aaaatcgttc cgctgatcgc ttatatgagc aagtttttta ccctcaaggc
     6241 cggtgacgtt gtgctgacag gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga
     6301 gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc
     6361 gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta
     6421 acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa accctggacg
     6481 aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata
     6541 aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca
     6601 atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cccgactgca
     6661 ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct accaatgacc tgcgcttatc
     6721 gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc gctgcttact ggttcgaaag
     6781 cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga
     6841 ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga
     6901 tgttgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag ccctgttttt
     6961 gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg taactctcaa
     7021 tctttaaaaa acgtctgtat ttttaaatgt gataaacttt ataccttgag tacatggaga
     7081 gaataattgc tgatcagact tcaggtacct caaagagtgt cttcttaccg tgtcttttct
     7141 gatactcatt acaggtgtta atcattttat tggccgcttc ttttagcaaa gaaagcatta
     7201 aatcatcata atcaacatag aatctggttg tttcagtttc cgtttttatc tccccgatgg
     7261 cggctatttc ggtggttaat ttcaatttgg cggcatcgat atctttattc gcttgtttaa
     7321 ccgtgttaga cagggtggta aagaaactct gcacagattt taacttgttc ttcaattctg
     7381 gaatcagttt cccttcaact acgcccgcag caatagaata ggaaatgatt aaaccaaatg
     7441 gaccggcgac gacaccggct gcggcaccgg catacgcttc cttcctgatt ttatctacct
     7501 gtgactggaa atagctgctt ttttctgaaa aatcattggt taactggcta tctaacgcca
     7561 gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac cagcagggat ttttgcgctt
     7621 cattcagctt cgtgatgccg tcatccagta ccttaatgag aatgtctttc tgggcggatg
     7681 ctttcttctc attatactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa
     7741 caccacacca ttcatacact gtttgggttg tttcaaaata cttatcctgg ctatccatt
//
LOCUS       DBFIFS010000206         7761 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.206, whole genome shotgun sequence.
ACCESSION   DBFIFS010000206 DBFIFS010000000
VERSION     DBFIFS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.206"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..786
                     /gene="gadX"
                     /locus_tag="N7A07_003936"
     CDS             <1..786
                     /gene="gadX"
                     /locus_tag="N7A07_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU2478507.1"
                     /translation="RHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRD
                     TLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKR
                     ALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPS
                     LLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNY
                     YGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
     gene            1154..1882
                     /gene="gadW"
                     /locus_tag="N7A07_003937"
     CDS             1154..1882
                     /gene="gadW"
                     /locus_tag="N7A07_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU2478508.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2245..5358)
                     /gene="mdtF"
                     /locus_tag="N7A07_003938"
     CDS             complement(2245..5358)
                     /gene="mdtF"
                     /locus_tag="N7A07_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU2478509.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5383..6492)
                     /gene="mdtE"
                     /locus_tag="N7A07_003939"
     CDS             complement(5383..6492)
                     /gene="mdtE"
                     /locus_tag="N7A07_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU2478510.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6600..6878
                     /locus_tag="N7A07_003940"
     CDS             6600..6878
                     /locus_tag="N7A07_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478511.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6879..7406)
                     /gene="gadE"
                     /locus_tag="N7A07_003941"
     CDS             complement(6879..7406)
                     /gene="gadE"
                     /locus_tag="N7A07_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU2478512.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2005 a         1816 c         1844 g         2096 t
ORIGIN      
        1 agacataaat atattctcac catggttaat ggtgaatatc gctattttaa tggcggtgac
       61 ctggtttttg cggatgcaag ccaaattcga gtagataagt gtgttgaaaa ttttgtatta
      121 gtatcaaggg atacgctttc attatttctc ccgatgctca aggaggaggc attaaatctt
      181 catgcacata aaaaagtttc ttcattactc gttcatcact gtagcagaga tattcctgtt
      241 tttcaggaag ttgcgcaact atcgcagaat aagaatcttc gctatgcaga aatgctacgt
      301 aaaagagcat taatctttgc gttgttatct gtttttcttg aggatgatca ctttataccg
      361 ctgcttctga acgttttaca accgaacatg cgaacacgag tttgtacggt tatcaataat
      421 aatatcgctc atgagtggac actagcccga atcgccagcg agctgttgat gagtccaagt
      481 ctgttaaaga aaaaattacg cgaagaagag acatcatatt cacagttgct tactgagtgt
      541 agaatgcaac gtgctttgca acttattgtt atacatggtt tttcaattaa gcgagtcgca
      601 gtgtcctgtg gatatcacag cgtgtcgtat ttcatttacg tctttcgaaa ttattatggg
      661 atgacgccca cagagtatca ggagcgatcg gcgcagggat tgccgaaccg tgactcggcg
      721 gcaagtattg ttgcgcaagg gaatttttac ggcactgact gttctgcgga aggaataaga
      781 ttatagagtt ttactcagac ataaaaaaaa cccggcatag gggaccggga agaggatagt
      841 ctgccgtctc cagactaata aaccgttata acactccctg ttggcacggg aaactttgtg
      901 ctctcagtaa gttaaatata acttttactg gaaataagat cagccatttt tttataaaca
      961 taagctatac gctgtgcgaa aatatattcg tgttgcattt acttattatc aattaactgt
     1021 tatgcaaaac tactttgtgg ataaattttg gtcctaccaa atttggcagt ttttgcgcta
     1081 agaaacagtc tggcatcatt tcattagtat actgaaattg aaataatcgc agtatgaaat
     1141 ataagggata atcatgactc atgtctgctc ggtgatcctc attcgtcgtt cattcgatat
     1201 ttatcatgaa cagcaaaaaa tatcgctgca taacgagagt atcctgctgc tggagaaaaa
     1261 tttggcagac gattttgcgt tttgttcacc ggatacgcga cgactggata tcgatgagct
     1321 gacagtttgc cattacttac aaaatattcg tcagctacca cgcaatttag ggttacatag
     1381 caaagaccgt ctgttaatta accagtcacc ccccatgccg ctggtgacgg cgatttttga
     1441 tagcttcaat gaatccgggg taaattcacc gatactgagc aatatgctct acctttcctg
     1501 tttatcgatg ttttctcata agaaagaact gatcccctta cttttcaata gcatcagcac
     1561 tgtttcagga aaagttgaac gccttattag ctttgatatc gccaaacgtt ggtatctgcg
     1621 cgatatcgcg gaaagaatgt ataccagcga gagtctcatc aaaaaaaagt tgcaggatga
     1681 aaacacctgt ttcagtaaaa tattactcgc ctccaggatg tcgatggcca gacgattact
     1741 cgagttacgt caaattcctc tgcatactat tgcggaaaaa tgtggctata gcagtacgtc
     1801 gtactttata aacacatttc gacaatatta tggtgtaacg ccacatcagt ttgcgcaaca
     1861 ttcgccaggt accttttcct gacatatttt gcatttgaat attggtcagg atctcacacc
     1921 tgcttcatgt gaaactcttc cctgatgatt tctgccgggc taccggctag ttctctttcg
     1981 cagtttgcca ggcggtatag gtattttcag gaggaaaaga ctcggcatgt ttgggattat
     2041 taagctgaca attcatacga ttacgggaat gtgggtcagt attgtgctgt tcaaattgat
     2101 gacgaatggc tggagtggtt tctactttca gtgctgtgta ctaagcctgg tattcctgac
     2161 ggttagctgg ttgttgagtg gtgaatggtt agcaggaaag agtaaggctg aaccttcacg
     2221 ttcaacctta ctctcattta cacgttacgc ttttttaaag cgggcaaaga gatgttccac
     2281 tacaacgaaa aagaccggaa caaagtaaat tgccagcact gttgcggcaa acatcccgcc
     2341 catcacgccg gtacctaccg cgttttgcgc gccagaaccg gcaccatgac tgataaccag
     2401 cggcagcacg ccgagaataa aggccagaga ggtcatcagg attgggcgta aacgcatccg
     2461 cgccgcttcg atgattgcct ctatcggcgt tttcccttct ttctgcatca tctcaacggc
     2521 aaattcgaca atcaggatgg cgttcttggc ggaaagcccg atggtggtca gcaaaccaac
     2581 ctggaagtag acgtcattac ttaagccgcg cagatcggtg gccagtaatg cgccaacgac
     2641 gcctaacgga acaaccaaca tcaccgagaa cggaattgac cagctctcat aaagtgcggc
     2701 gagggcgagg aacaccacga ccagtgaaat cgcatacagc gcaggagcct gatttgagga
     2761 taacgcttcc tgatacgaca gtccggtcca tgagtagccg acgcctgccg gaagtttagc
     2821 gaccaggtct gccataaatt tcatggcgtc accggtactt ttcccggccg ccgcttcacc
     2881 taaaatctcc attgacggga tgccgttgta gcgttccagt cgcggtgaac cataggtcca
     2941 ttcggtagac gagtaggcag aaagcggtgc catcgtgcca gaggcgttgc gtacatacca
     3001 ttggttgatg ttatccggca acatacggaa cggcgtgcct gcctggacat acactttttt
     3061 cacccgcccc tggttgagga agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt
     3121 ctggttgata tcagacagcg ccacacccat tgcctcagct ttcgcggcgt tgacgttcac
     3181 tttgaacatc ggcgtatctt ccaggccgtt cgggcgtacc ccggtgacct gattcggtga
     3241 ttgcgctgcc agtgataaca gctcgtttcg tgcctgggtt agtttttcgt gccccaggtt
     3301 accgttgtcc agcagttcca tatcaaaacc tgacgcggta cccagttcag ccaccgcggg
     3361 taagttgaac gggaagacga cggctttatt gatactgctt agcgcaatca ttgcccgctg
     3421 aatgatcgcg gtaaccgagt tttcctcacc gacacgttca gaccacggct tgagactgat
     3481 aaacgccaga ccgttgtttt gcccctggcc gctgaagcca aagccgccaa cggtaaacac
     3541 cgactgaaca ttatctttct cttttgtaag ataataatcc gtcacctgtt gcagcacttt
     3601 cgtggtgtta accatggtgg caccggaagg taactgcgcg gtggtcataa ataccccctg
     3661 atcctcttct ggtaagaaag aggtcggcgt gcgcaggaac agcaccgcca tcccggcgca
     3721 aatcagcagg tagatcacca tgtagcgacc ggtacaacgc aacagtgagc gggtgctatc
     3781 ggtatagtgc tgagttgatt tttcaaacag cgtgttgaag cgtgcgaaca gggcattagg
     3841 tttgtgaccg ccttccggcg cggctttcag aatggtggcg cacagggcag gggtcaggct
     3901 cattgccaca aatactgaaa gcagcatgga ggagatcagc gtgatggaga actggcggta
     3961 gatctccccg gttgcaccgc tcataaaggc catcggcata aacactgcgg aaagaacaac
     4021 ggcaataccg accagcgcac gttggatctg ccccatcgat ttatgcgtcg cttccttcgg
     4081 tggtagctta tcttccgcaa tgacacgctc gacgttctcc accaccacga tggcgtcatc
     4141 caccagtaac cctatcgcca gcaccatccc gaacatagtc aacgtgttga tggtgaaacc
     4201 gaccgccgac aagatcgcaa acgtcccgag aataaccacc ggtacggcaa tcgtcgggat
     4261 gattgtggca cggaaattct gcaaaaacag atacatgacc aggaagacga ggatgatagc
     4321 ctcaaccagt gttttgaaaa cttcctgaat agaaatttcg ataaacggcg tggtgtcgta
     4381 aggataaacc gtcttcagac ttgccgggaa ataggctgat aagcggttca gttcctcttt
     4441 gactgcccgc gaggtatcca gggcgtttgc tccggtagcc agtttgatgg cgatcccggc
     4501 agcaggtttg ccgttatagc gcgccacggt ggaataatct tccgccccaa gttcgacgcg
     4561 ggcgacatca cgcagcagca cttgcgaacc atcttgctga actttcaaca ggattttgcc
     4621 aaattcttcc ggcgtttgca gacgcgtctg cacaatgatc gaggcgttta gttgctggtc
     4681 tgccgcctgt ggcatgccac ccagttgacc accagaaatc tggttgtttt gcaccttaat
     4741 ctgggaaata acatcggaag gtaccagatt gtatttattg agtttttgcg ggtccagcca
     4801 gatacgcatg gcgtactcgg aaccaaagag ctgtacgcta ccaacgcccg cggtacggct
     4861 tagcgggtct ttgatattag acgctacata gtccgcgata tcgtactggt tgaggctgcc
     4921 gttatcagaa ataaacgccg ctaccatcag gatattactg ctcgatttat cgacgctaat
     4981 cccctgctgc tgcactgctt caggtaatga aggcatagcg agttgcagtt tattttgcac
     5041 ctgaacctgt gcgatatcag gagatgtccc agtctcgaag gtcagagtga tagaggcatt
     5101 acccgccgca tcactggttg aagacatgta catcaggcca tcaagcccat tcatattttg
     5161 ctcaatcacc tgagtgaccg agtcttctac cgtttgcgca tcggcacccg gataggtagc
     5221 gctgacggta atggtcggtg gcgcaatctg cggatactgc gcaaccggta agttcatgat
     5281 cgccagacca cctgcaagca tcataataat ggcaagtacc caggcaaaaa ccgggcgatc
     5341 aataaaatag ttagccatga aagtcccctt aagcctgcaa cgttattgtt tcgattcggt
     5401 gctggcgttt tcctggctgg aggaaattgc tcgtgctttg atacccggac gaatgcgttg
     5461 caaaccggaa acgatcaccc gatcgccagc ctgcaagcca gaggtgacaa cccactggtc
     5521 gccgatggct ttgctggctt caatttcgcg tagcttcacg acatcgtctt tatccagaat
     5581 gagcgccgtt gctttacctt gggcgttgtg ggtgacgcct tcctgcggca ccagtaatac
     5641 attctggcgg ctaccttcat ccactaatgc cgtgacatac atgccaggca gcaagtcacc
     5701 atttgggttg gggaaaatcg cccgtaacgt cacggagccc gtggtttcat caaccgtcgg
     5761 gtcggagaat ttcagcgtgc cggtctggct gtaacgttta ccattttcca gattgagctg
     5821 tactggcgta ctgccctgaa cctgtttgat ttgcccactg gcgacctctt ctttcatgcg
     5881 taagaaatct tgcaccgact gcgtgagatc gacataaatc gggtccagac gttgtacggt
     5941 aaccagcgaa tctgcctgat tagcggtaac gagtgcgccg acggtcaccg acgatttccc
     6001 gctgacgccc gtaatcggcg aggtgacatt cgcgtattgc agattgatcg tcgcctgttc
     6061 aacagccgct ttggcgacgg tgacattggc ttctgcttca ttcaactggg tgcgcgcggt
     6121 gtcgtaatcc tgacggctaa cgtagttggt cttcagcaac gatgcctggc ggttaaaggt
     6181 gatgcgggca ttgctggcgg tagagagcgc tttcgccagc gagcctttgg cggagtttag
     6241 ctcggcctgt aaaggtgcag gatcaatctg atacagcgaa tcgccctggt tcactttatc
     6301 gccttcgata aagttgcgtt taatgataat accgcccacc tggggacgta tctcggcaac
     6361 ttcataagga acggttctac cgggcaattc gctcaacaca ttgaccgacc caggggagag
     6421 tgtgacgaca ccgacctcag gcgtcatggc ggcggtgttt tccgccgatt tgtcatcgca
     6481 ggcggtgagc atcgcgccgc agaataacaa cggtattaac agctttcttc ttctgttcat
     6541 tttagtccct gaaaattctt gagaaataac ggttctttaa attgcgcgcg aggagagata
     6601 tgagggattt acagaccagc ggcatagtgg ggttgtcagc cagtaaggtg ggatacaggc
     6661 atagtgatcg acatggtgag gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttc
     6721 ctgccagcat tcgggcaacg aagggttcta ctggtggata cacataccag gggaataaag
     6781 tcgttttagc ctctccttta gtaatcaccg ggggtaatga aatcagcatc tgcatccctc
     6841 gtcatgccag ccatcaattt cagttgctta tgtcctgact aaaaataaga tgtgataccc
     6901 agggtgacga tgtcgctcat acgtcttagt ttcagcttgt acataatgtt gaatttctga
     6961 atctttaatg tttgttgcga aatattgagc gtcgacgtga tattgctttc atttttattt
     7021 ttcagaatca gttgcataat tttgaactga ttatgtgaaa aacataattg tgaattctta
     7081 tggggcaagt gtttaccata agtaatctca agaagatttt tgatactttc tttgcggcta
     7141 acaaaataaa taggcgcttt agcttttagt ttactgatgt gataaggtgc caaaacaata
     7201 attctctcgg catctaattt ctccagaaat ttaatcgctt cttcatcaag gatatgattg
     7261 tgataggtat caatgataac cttgaagggt ttattgccta cttttttgat ctctgacagg
     7321 gaattgattt ttatcatttc gtgattatct ttcaactgcc aaaagccctg taaaagaaaa
     7381 gaatctttcg tcatgagaaa aatcataact tgctccttag ccgttatcgt ttcgaacatg
     7441 tcatcctttt cattatttac atccttgtcc gaatcgttgt tcaatatagt aaacgcctat
     7501 aagaactttg cccttcgtga actaatacgt ctattaacac taatcaatac ttcagattta
     7561 tccatcacgc actccatata ttgatcctaa gtaagattcc tggttgttat cagcttgtaa
     7621 atagtaaaca aaaagttaac gtttgcggat tactgcccaa gaataagtta tcaataacca
     7681 gtttgtgatc tctgaagaat attactaaag ttaaaaagta atccgattta aatatcgagt
     7741 ctccttgttt cgacttaagc t
//
LOCUS       DBFIFS010000207         7748 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.207, whole genome shotgun sequence.
ACCESSION   DBFIFS010000207 DBFIFS010000000
VERSION     DBFIFS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.207"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1018
                     /gene="espL4"
                     /locus_tag="N7A07_003942"
     CDS             <1..1018
                     /gene="espL4"
                     /locus_tag="N7A07_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGU2478513.1"
                     /translation="NYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGL
                     FLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDAD
                     MLKIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALP
                     SLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDK
                     KNMKPLLLANNSNEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQHK
                     APQKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASP
                     HTVR"
     gene            complement(987..2723)
                     /gene="aceK"
                     /locus_tag="N7A07_003943"
     CDS             complement(987..2723)
                     /gene="aceK"
                     /locus_tag="N7A07_003943"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU2478514.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2907..4211)
                     /gene="aceA"
                     /locus_tag="N7A07_003944"
     CDS             complement(2907..4211)
                     /gene="aceA"
                     /locus_tag="N7A07_003944"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU2478515.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4241..5842)
                     /gene="aceB"
                     /locus_tag="N7A07_003945"
     CDS             complement(4241..5842)
                     /gene="aceB"
                     /locus_tag="N7A07_003945"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU2478516.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6111..7040)
                     /gene="metA"
                     /locus_tag="N7A07_003946"
     CDS             complement(6111..7040)
                     /gene="metA"
                     /locus_tag="N7A07_003946"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU2478517.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7197..7640
                     /locus_tag="N7A07_003947"
     CDS             7197..7640
                     /locus_tag="N7A07_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU2478518.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         1914 a         1936 c         1953 g         1945 t
ORIGIN      
        1 aaattatggt aatgcggata tcgttgagac tatttttaat tcattgtcag agccaggata
       61 tgaaggatta ctctcgaaaa aaaatctcat gcatattctg gaggcaaaag ataaaaatgg
      121 tttttctgga ttatttttag cgatatcacg taaggataaa aatgttgtaa cctcgattct
      181 gaacgcctta cctaaactgg ccgcaacaca tcatttagat aacgaacaag tgtataaatt
      241 cctgagtgcc aaaaatagta cgtccagcca tgttttatac catgttatgg cgaatggtga
      301 tgccgacatg ctgaaaattg ttttggacgc gttatctttg ttaattcgca catgtcattt
      361 gactaaagaa caggtactcg atctcctgaa ggcaaaggat ttttatggtt gcccaggact
      421 atacctggcg atgcaaaatg gacatagcga tatcgtgaaa gttattcttg aagcattgcc
      481 cagcctggcc caggaaatta acatttcagc ttccgatatt gtagatcttc tgaccgctaa
      541 aagtcttgcg cgcgacacgg gtttgtttat ggccatgcag cgcggacata tgaacgttat
      601 taatactatt tttaacgcat tacccactct gtttaatacg tttaaattcg ataaaaaaaa
      661 tatgaagccc ctcctcctgg caaataattc taatgaatac ccaggtttgt tttcagcgat
      721 acagcataaa cagcaaaacg ttgtagagat ggtttatctt gctttatctg accatgcacg
      781 cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa
      841 atactctgcc tttgagttgg cttgtgaatt gggtcaccgg gttattgctg aattaatctt
      901 taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc
      961 ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat
     1021 ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt
     1081 agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca
     1141 atacgcgggt cggcgcatag ccaatggcga aactcttccg ggaaaacatc gcccggcgag
     1201 acgctgtacc acggttcgct ggcaagttcg tcttccgggt agcgcggcgg cgggatatcg
     1261 cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg
     1321 tgacgggtga caccgaagtt tttaaacagc atgtcgcccg ggaaaatgtt agcagcggca
     1381 agctggcgaa tggcattgcc gtactcttca atggcgtcgc gcagctgctg accttccact
     1441 tgttccagcc agatattgag cggcaccatc cgccgctcaa tataaagatg gcgaatcaca
     1501 atttgttcgc cgagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat
     1561 gccggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt atccgccatt
     1621 cggcccacgc gatcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac
     1681 atctctttct gcggcgcgaa cttgtctttg atgactttaa atacacggtc aaagcccggc
     1741 agcgtgaaca ccaacatcac catgccacga atacccggcg cttcaatgaa ctgctcatta
     1801 cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag
     1861 ccgatagcca tatacaattc agcggtggtt ttacctggca gaatttcccg taaccactcg
     1921 accagcgctg cgggcagcgg cgcataaacc ataaaataag aacgcgcaaa gccaaaaaca
     1981 atgctcgctt cggcggtcgt cgtcagacag gtatcaataa acagttcgcc gtcatccgtc
     2041 tggtggatcg gcaataaaaa tggcaatgtg ccggaaggcg ttattagttt gcccaccagc
     2101 caggcggctt tattacggta aaacagttcg ttcgccacct gtaaatgact tttggagagg
     2161 ttttctggcc ccaacgtttc cgtcagatgg cgaataatgt aatggatgtc acggctttta
     2221 ttctgccagt gcagacgcaa cggcaggtcg ctgataacgc gcatcagcag agattcccag
     2281 ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct
     2341 ggctgggagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga
     2401 cagtacacgg agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc
     2461 cgggtgtaat gctctttgac gcgcagtaaa aattccgcgt ccgtgctttg gccgttggta
     2521 atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt
     2581 ttcatcgcct gctggacagc atgccagtcg gcttgttcga aacgctgctg cgccccggaa
     2641 gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc
     2701 agtaattcca ggccacgcgg catttagcgc cctcatcagg agcagagaat tgccggatgc
     2761 ggcgtaaacg ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca
     2821 agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtatatgcc ttatccggcc
     2881 tacagtcagc aacgtttgta ggttgcttag aactgcgatt cttcggtgga gccagtcagc
     2941 gcggtaaccg acgacgtgcc gccctgaata atggtcgtca ctttatcgaa gtaacctgta
     3001 cccacttcct gctggtgaga tacgaaggtg tagccatctt tcgcggcggc gaattccggc
     3061 tgctgcactt tctcaacata gtgcttcata ccctcgccct gggcataggc gtttgccaga
     3121 tcaaacatgt tgaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag
     3181 cccatatccg acagctgctg ctggaagctg gcaatagtgt tgtcgtcgag gtttttctgc
     3241 cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata
     3301 gcttccgcaa agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccataccagg
     3361 tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta
     3421 cggaagaagc cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa
     3481 tcggaggtga tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg
     3541 cccgtcacgt cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc
     3601 accaaaactt tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag
     3661 tgaactgccg ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca
     3721 ccgccaaaac cggcttccgc gtcggcaacg atcggcagga agtaatcgac atagcgcgga
     3781 tcgcccggct caatgcccgc ggaccattgg atctgatccg cacggcggaa agtgttgttg
     3841 atccgctcca ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg
     3901 ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac cgcttcaata
     3961 cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag
     4021 ccttttttcg actcgccatg cagcagacgc cacattttcg ccgcgcccag ttgcgccagc
     4081 gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcgct gtatgggcga
     4141 gtaattcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta
     4201 cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc
     4261 ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg
     4321 cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt
     4381 tcatctcttc gcccagcatc tggcggaaca aggctttagt taccggtttg ccattgctca
     4441 acgttttttg atgatggatc cactgccaga ttgaggtacg ggaaatttca gccgtcgccg
     4501 catcttccat caggccataa atcggcacgc agccgttgcc ggagatccat gcttcgatgt
     4561 actgcacagc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag
     4621 gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact tcaagctgat
     4681 ttttacggga gccgagaatg tcgttgaata ccgccattgc cgtgtccgca aggcctgggt
     4741 gagcgatcca tgtgccatcg tgaccgttat tggcttccag cgatttatcc gcttttactt
     4801 tgttgagcac ctggttattg tgctcttcat ctttgctcgg aataaacgcc gccatgccgc
     4861 ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag taagcattca
     4921 ggaatggttt atccatcgtt actgcctgtc tgtctggcag gacgcgatcg ggatagtttt
     4981 tcaacgtttt gatatagctg aagatgtaat cccaacgacc gcagttcaga ccaacaatat
     5041 ggtcacgcag cgcgtgaagg atttcatcca tctggaacac ggcgggcagc gtttcaatca
     5101 gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga
     5161 cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac
     5221 cactgccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca
     5281 ggctgccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc
     5341 gtacccgaca aatcaaaacc gctggattgg gcttgagctg gtaaattttg cctgcttcat
     5401 tggtgtaact gatggtgccg ttaaccgcat cacgcaggtt aatttgcccg tcgatcactt
     5461 tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt
     5521 tgagcgcgtt gatcaccatc ttgcgctcta cggatccggt aatctctacg cggcgatctt
     5581 ctaagtccgc aggaatcccg cgaattttcc agtcagcatc acgaatggaa gctgtttccg
     5641 aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca
     5701 gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt
     5761 cggcagtaag aatttgcttc tcctgctcgc catacggcct tatgaaagcc agttcatcgg
     5821 ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttccccac
     5881 tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa
     5941 atcaaaatca aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa
     6001 tatgaataaa atttattcac ctactgaggt gtgtttcgga aataaaaaag gcacccgaag
     6061 gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc
     6121 gttggattca tgtgccgtag atcgtatggc gtgatctggt agacgtaata gttgagccag
     6181 ttggtaaaca gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatca
     6241 ttgtgcggga aatagttata cggtacttcc gggtctagtc cggcttccac atcgcggaaa
     6301 tattcctgcg ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc
     6361 ttatctttac tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga
     6421 tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccaag
     6481 aatgaatcat caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa
     6541 acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgct
     6601 gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc
     6661 acctgtttga tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca
     6721 ccggttacaa tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag
     6781 ttgttcagat gctctgcggg cgtgttacgc gattcacggg aatcgatgcg caacagctga
     6841 atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc
     6901 ttcttcggca tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca
     6961 cgagaagttg tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg
     7021 tccggcacac gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca
     7081 tccagatagc tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga
     7141 gaaagtttca aggaaaagac tacgttagaa tataagaatg actaaaagga gagaaaatgg
     7201 ttattagtat tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc
     7261 gatctgtcga tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc
     7321 tggttcgttc ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggatcagc
     7381 cggttgggtt tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg
     7441 tgcgcggctg cggcgtgggc cggatgctgg tgaagcatgc gctctcaatg gccccggaac
     7501 tgacaaccaa tgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt
     7561 ttaaggttac gggacgctct gaggtggacg atttggggaa accgtatccg ttgctggatc
     7621 tggcgtatgt gggggaataa gctgccccct atcgtgcctg cgtaagctgg ataaaacgcg
     7681 ccagcgtcgc atccggcatt tttttgcacc aacgcaaaaa ggccatccgt caggatggcc
     7741 tttcgctt
//
LOCUS       DBFIFS010000208         7715 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.208, whole genome shotgun sequence.
ACCESSION   DBFIFS010000208 DBFIFS010000000
VERSION     DBFIFS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.208"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            143..361
                     /locus_tag="N7A07_003948"
     CDS             143..361
                     /locus_tag="N7A07_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU2478519.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            578..820
                     /locus_tag="N7A07_003949"
     CDS             578..820
                     /locus_tag="N7A07_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU2478520.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1150..2079)
                     /gene="fdhE"
                     /locus_tag="N7A07_003950"
     CDS             complement(1150..2079)
                     /gene="fdhE"
                     /locus_tag="N7A07_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU2478521.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2076..2711)
                     /gene="fdoI"
                     /locus_tag="N7A07_003951"
     CDS             complement(2076..2711)
                     /gene="fdoI"
                     /locus_tag="N7A07_003951"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU2478522.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2708..3610)
                     /gene="fdoH"
                     /locus_tag="N7A07_003952"
     CDS             complement(2708..3610)
                     /gene="fdoH"
                     /locus_tag="N7A07_003952"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU2478523.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(3623..6673)
                     /gene="fdnG"
                     /locus_tag="N7A07_003953"
     CDS             complement(3623..6673)
                     /gene="fdnG"
                     /locus_tag="N7A07_003953"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6086..6088),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU2478524.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6867..7700
                     /gene="fdhD"
                     /locus_tag="N7A07_003954"
     CDS             6867..7700
                     /gene="fdhD"
                     /locus_tag="N7A07_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU2478525.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1780 a         1998 c         2056 g         1881 t
ORIGIN      
        1 tgaaacaact gcaacgctct tattatttct ggaaaacgtc tcgcaaaaac aaaacaacat
       61 gcaaaaatcc gctttcgtta attaattttt cggcgtaatt ttcgcacata cattaatgag
      121 taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt tatcagaaga
      181 ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat ctgcaatttt
      241 acgcgaagcg gtagagcaat atctggaacg tcagcaattc ccggtgcgta aagcaaaggg
      301 aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc ataaggaatg
      361 acaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt attaatagaa
      421 ggctgtttta cttgaactta taataactgc aactgttaca tcatatctgg aaaacgcctc
      481 gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcgacaa gggtactttt
      541 ctctccatag atatcaaatg atattggtca aatatttatg aactctctgg caggtattga
      601 tatgggcaga attatactcg atttatcgaa tgaggtgatt aagcaacttg atgatcttga
      661 ggtgcagcgt aatcttcctc gtacggagct attacgggaa gcggtagatc aatacttggt
      721 aaatcaatcg caaacagtaa gaaccagtgc ttttggcata tggcaagggt gtgaggaaga
      781 tggtgtcgaa taccagcgta agctgcgcga ggaatggtaa tgggaaaaaa tgatatcaat
      841 cagattgctg acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc cttacccggc
      901 ctacaaagtc gtataaattc aatgtattgc agtttattgg taggcctaat aagcgcggcg
      961 catcaggcaa tcttacgctt atcatcagtc tcatacccat aaccttattg atgtgggggt
     1021 gggtaaattg aggcaaaaca ggcactggcg gcatagcttg aggtggtaac gcggaatacg
     1081 agagatggtg tcgtcacacc ctatgagctg taatccgggc gaccagcgcc acccggaaaa
     1141 cgtcagagat tactcccctt cacccggaaa caggaacggg ttgatggaac tgcgggcata
     1201 gccttcttgc tccattcgcg cgtccagtac cagagaggcg aggtcatctg ccacggcttc
     1261 aactttcggt tctttttcct gatagagaat tttcaggtaa gtgccgcagt catcgcagct
     1321 ttcggcttta atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg
     1381 ctcgcagttg ctgcatttta cgcgcacaac gtgccattca gtttcacaca ggttgcagtg
     1441 caggtaacgt agcccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagaacc
     1501 acaaaccggg caatattgac gttgctcgcc gtattcagcg cgggctttgc cggggatcag
     1561 attggccatc tgcgcccagt agagcgacag tgcagcccag ataaacggcg ctttatcgct
     1621 gctgacggac gagaaatcag aggcaaacag tgcgctggcc atatcttcca gctcctgagt
     1681 cgatgccttc tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag
     1741 ctcagcaatc agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat
     1801 atccagcggg ggcttgcctt gtgcgctggc ttctttaatg cgcgcagtca gatccatctc
     1861 cagcggatgg tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag caaagcgcag
     1921 gtaatcaccc agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta
     1981 taaattcttg agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct
     2041 cgaacccagc tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc
     2101 ttgcggacct cacggtacca gcgcgggtga tgtttcttcg cccaagcgct ggtaacccat
     2161 ccttccacca tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg
     2221 ataaccacaa ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc
     2281 gggattgaga aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc
     2341 agaaccagga aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg
     2401 gtgtcaccta cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca
     2461 tcccgattga ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac
     2521 ataaccacgc cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc catgatttgc
     2581 atcaaccagt tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag
     2641 cagaaggcgg tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca
     2701 cgtcgtttca tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg
     2761 catggttcgg accgacaccc acgtagtgga agatactggc tgcgaaggta gccgcaaagc
     2821 caacagctgc gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg
     2881 ggttctccgg caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacataa
     2941 cgtgtgtacc accgacgcct gccggatcgt acaggcccgc attgtcgtaa ccgcgggttt
     3001 tcagctccgc cacgcgctcg ctcgccagcg ttttcatcga ctctttcgta ccgaagtgaa
     3061 tcgcgcccgt tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc acgcggtcaa
     3121 cgcacagcgt acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg
     3181 gacagcccgc aatgcaataa ccgcagccaa tgcactgctc ggactggaag tcgacgatac
     3241 cgttggcata ctgaataatt gcaccttccg ccgggcacgc tttcagacag cctggatcgg
     3301 aacagtgcat acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt
     3361 ccgagaagcg catcaccgtc cacgatttgg cgcttaaatc attggggttg tcgtacaccc
     3421 caatgttatt gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg
     3481 ctttacagcc gatacaggtg gtgacgtcga tgagtttcgc cacttcttcc tggaagtccc
     3541 gcgcctgagg cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgagatt
     3601 gataagccat atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact
     3661 ccggcgtctg cgtgttcgca tcaccgacga atggcgtcaa cgtattggca ataaagcctt
     3721 ttttcgcaac accttcatag ccccagtgaa taggaatacc gatggtatcg atatctttgc
     3781 cgttcgcttt cagcgtgcga atacgtttgg tcaccaccgc tttggctttg atatagccac
     3841 ggttggagga gactttcacg gtatcgccct gggcaatgcc aagtttattc gccagcgact
     3901 ccccgatttc cacaaactgc tctggttgca aaatcgcgtt caacagcgcg tgtttggtcc
     3961 agtagtggaa gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt
     4021 tacccaatgc ttcggcgtcg tctttaaaga tacgtgcagc cgggttcgag ataacgtttg
     4081 gatgcagcgg gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac
     4141 cttccgccat cttatcgagg gcaaacagac gccccatgcc ttcctgctgc atgataaacg
     4201 gcccgacgcc gctacccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact
     4261 tagtgccgtc ccatttcagc aactgacgct tcggatccca cgggttaccc tgcggatctg
     4321 cggaggcgcg gttatacaga atgcggcggt taagcggcca tgcccatgcc caacccagcg
     4381 tgttaccgag gccagacgga tcggcgttat cacggcgtgc catctggttg ccttccggcg
     4441 tccagctacc ggcgaaaatc cagcaaccac aggaagttgt accgtcatcg cgcagttggg
     4501 caaacgagct aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa
     4561 tatcggccag cgccttaccg ttgctctcca tcgctacttc ttccgacttc ggctcatgcg
     4621 gaatggcgta gttccaggtc atgttcagca cctggtccgg gttcgcgcca ccctgttcgg
     4681 catacatctt gcgcaagcgc aggaagatac cggagaggat ctcgccatca gtcagcgcaa
     4741 tccccggggc gtccgcacct ttccagtgcc actgcaacca gcgacctgag ttaacgattg
     4801 aaccgttctc ttctgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg
     4861 acgagtcaac ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca
     4921 gcgggtcgat ggtgacgagg aacttcagtt tcgacagaca actgatcact ttgtttttgt
     4981 tcgggaatga ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccttctt
     5041 tcatcatctc gaagtattgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa
     5101 agccccagct attttctgcc gtcgctttat caccaaagaa ggccttcatc atagagacga
     5161 agaatttcgg gtagttgccc cagtagtttc cctggccttc cagcagcggt tttggcgtgt
     5221 tggcggtaag gtaggtttgc agatcggtct gcttctcgct tggcagcgtc atgtaacctg
     5281 gcaggctctg cgacagcagc cccaggtccg tcagcccctg aatattggag tgaccgcgca
     5341 gggcgttaac gccgccgcca gccatcccca tgttaccgag cagcagctgg atcatcgcca
     5401 tcgtacgaat gttttgcgca ccaacggagt gttgcgtcca gccgagggca tacaggaacg
     5461 aggcagtttt atcgtgagca ctggtttctg cgatgtattc gcagactttc aggaacgcgt
     5521 cttttggcgt accacagatg ttttcaacca catctggcgt gtagcgggaa acgtgctgtt
     5581 tcagcaagtt ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg gcgaagccgt
     5641 tttcgtccag ttcataagtc caggaggatt tatcgtactt gcgtttttcc gcgtcgtagc
     5701 cggtgaacag gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat
     5761 aggcttcagt gtattcgcgg ttgaattttt cattgttcag caggtacagc aatacgcctg
     5821 acaggaaagc aatgtcagta ccggaacgaa taggggcata gtagtcagcc accgcagccg
     5881 tacgcgtaaa gcgaggatcg atcacaatca gcttcgcgcc attgtgaatt ttggcttcca
     5941 tcgcccagcg gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga
     6001 ggttggcgtt cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag
     6061 caagacttgc taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac
     6121 ccagcgcgcg ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac
     6181 acagcatccc ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt tgcgcaatgt
     6241 agttagcatc gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac
     6301 tgatttgctg ccatttatca gaacctggcg cacggtattc cggaaacttc agacggcttt
     6361 cggagtggat gaaatccacc aggccagcgc ctttcggaca aagtgcaccg cggttgaccg
     6421 ggtgatccgg atcgccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac
     6481 cgaggctgta catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt
     6541 cgcgggtgcg cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac
     6601 ccagtgccgc tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc
     6661 tgctgacctg catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt
     6721 gctgcgtgtg cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga
     6781 attggctcaa tttccctcca tccaggaagg gtttgtaaca gaataccata atgttggtgt
     6841 gtgtgttctt atctggttaa gagaaagtga aaaaaacaca gcaaaaagaa atcgaaaatg
     6901 tgacaaatat cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat ttacaacacc
     6961 cacggttaga tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac ggcatttcgc
     7021 atgtggtgat gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt
     7081 ccgaggggat aatagaaagt ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta
     7141 atggtcttga agtacaaatt gagctttcca gccgccgctt tatggggttg aaggagcgcc
     7201 gccgggcgct ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa cttaatgaca
     7261 tcggaaaacc ggtgcagccg ctaccgttca cccagacgtt tgatctcaac aaactggatg
     7321 atgcattacg tcatctcaac gattttcagc cagtgggaca actgaccggt tgtactcacg
     7381 ccgccgcctg gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc
     7441 atgtggcgct ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcagcaag
     7501 gtgcggtact ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg
     7561 gcgtagagat tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa gtggctgagc
     7621 gctgtaatct gacactggta ggtttttgta aaccgggtcg ggcaacggtt tatacccatc
     7681 cgcagcgttt gagcaattaa ttaaaataag tcctg
//
LOCUS       DBFIFS010000209         7693 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.209, whole genome shotgun sequence.
ACCESSION   DBFIFS010000209 DBFIFS010000000
VERSION     DBFIFS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.209"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..2413
                     /locus_tag="N7A07_003955"
     CDS             <1..2413
                     /locus_tag="N7A07_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGU2478526.1"
                     /translation="QKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNAS
                     AAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELK
                     DIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWG
                     FYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTAD
                     YYKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTL
                     CNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPIS
                     SIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWV
                     DIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFT
                     TEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGL
                     GLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLS
                     ILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGF
                     ELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQV
                     AHIVPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQ
                     CIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVF
                     CQQNN"
     gene            complement(2469..3610)
                     /gene="yfdE"
                     /locus_tag="N7A07_003956"
                     /pseudo
     CDS             complement(2469..3610)
                     /gene="yfdE"
                     /locus_tag="N7A07_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(3684..4628)
                     /gene="yfdV"
                     /locus_tag="N7A07_003957"
     CDS             complement(3684..4628)
                     /gene="yfdV"
                     /locus_tag="N7A07_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU2478527.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(4698..6392)
                     /gene="oxc"
                     /locus_tag="N7A07_003958"
     CDS             complement(4698..6392)
                     /gene="oxc"
                     /locus_tag="N7A07_003958"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU2478528.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6446..>7693)
                     /gene="frc"
                     /locus_tag="N7A07_003959"
     CDS             complement(6446..>7693)
                     /gene="frc"
                     /locus_tag="N7A07_003959"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU2478529.1"
                     /translation="STPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVT
                     RHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAID
                     HMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPL
                     VSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLD
                     KLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNW
                     ENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPC
                     APVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHT
                     AAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2231 a         1804 c         1625 g         2033 t
ORIGIN      
        1 gcaaaaagcg cctgacagtg aacaaacatt aaaaaaagga atgaaagttg ccattccata
       61 ttattatgag cttcattcgc aattaaaaga gatgtatccg gaggtagagt ggataaaagt
      121 cgataacgcc agcgctgcat ttcacaaggt caaggaaggc gaacttgatg ctctggtcgc
      181 gacacagtta aattcacgtt acatgatcga ccattactat cctaatgaac tgtatcattt
      241 tcttattccc ggcgttccga atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga
      301 acttaaggat attattaata aagcactgaa tgcaatcccc ccaagcgaag ttctgcgcct
      361 gaccgaaaaa tggattaaaa tgcccaatgt gaccattgac acatgggacc tctatagcga
      421 gcaattttat attgttacga cattatccgt tttattagtt ggcagtagcc ttttatgggg
      481 attctacctg ttacgctcag ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca
      541 aatatcattc cggaaagcgc tctcggactc cttaccgaat ccaacttatg ttgtaaactg
      601 gcaaggtaat gtcattagtc acaatagtgc atttgaacat tatttcactg ctgattacta
      661 caaaaatgca atgttgccat tagaaaacag tgaatcaccc tttaaagatg ttttttctaa
      721 tacgcatgaa gtcacagcag agacgaaaga aaaccgaaca atatacacac aggtatttga
      781 aattgataat ggcatcgaga aaagatgcat taatcactgg catacattat gtaatctgcc
      841 agccagcgaa catgctgttt atatttgtgg ttggcaagat attaccgaga cgcgtgattt
      901 aattcatgca ctcgaagtag agagaaataa agcgatcaat gcaactgtcg caaaaagtca
      961 gtttctggca acaatgagtc acgaaataag aacaccaata agctccatta tgggcttcct
     1021 ggaacttctg tcgggttctg gtcttagcaa ggagcaacgg gtggaggcga tttcactagc
     1081 ctacgccacc ggacaatcac tcctcggctt aattggtgaa atccttgatg tcgacaaaat
     1141 tgaatcgggt aactatcaac ttcaaccaca atgggtcgat atccctactt tagtccagaa
     1201 cacttgtcac tctttcggtg cgattgctgc aagcaaatcg atcgcattaa gttgcagcag
     1261 tacatttcct gatcattacc tggttaagat cgaccctcag gcgtttaagc aggtcttatc
     1321 aaatttactg agtaatgctc tcaaatttac caccgagggg gcagtaaaaa ttacgacctc
     1381 cctggttcac attgatgaca accacgctgt aatcaaaatg acgattatgg attctggaag
     1441 tggattatca caggaagaac aacaacaact gtttaaacgt tatagccaaa caagtgcagg
     1501 tcgtcagcaa acaggttctg gtttaggctt aatgatctgc aaagaattaa ttaaaaacat
     1561 gcagggtgat ttgtcattag aaagtcatcc aggcatagga acaacattta cgatcacaat
     1621 cccggtagaa attatccaac aagtggcggc tgtcgaggca aaagcagaac aacccatcac
     1681 actacctgaa aagttgagca tattaatcgc ggatgatcat ccgaccaaca ggctattact
     1741 caaacgccag ctaaatctat taggatatga tgttgatgaa gccactgatg gtgtgcaagc
     1801 gctacacaaa gtcagtatgc aacattatga tctgcttatt actgacgtta atatgccgaa
     1861 tgtggatggt tttgagttga ctcgcaaact ccgtgagcaa aattcgtcct tacccatctg
     1921 ggggcttaca gccaacgcac aggctaacga acgtgaaaaa gggttaaatt gcggcatgaa
     1981 cttatgtttg ttcaaaccgt tgaccctgga tgtactgaaa acacatttaa gtcagttaca
     2041 ccaggttgcg catattgtac ctcagtatcg ccaccttgat atcgaggccc tgaaaaataa
     2101 tacggcgaac gatctacaac tgatgcagga gattctcatg actttccagc atgaaacaca
     2161 taaagattta cccgctgcgt ttcatgcact agaagctggc gataatagaa ctttccatca
     2221 gtgtattcat cgcatccacg gtgcggctaa catcctgaat ttgcaaaagt tgattaatat
     2281 tagccatcag ttagaaataa cacctgtttc agatgacagt aagcctgaaa ttcttcagtt
     2341 gctaaactct gtaaaagaac acattgcaga gctagaccag gaaattgctg ttttctgtca
     2401 acaaaataac taaatagtgg ctcccacaat gttcaaatgt gggagctatt taccagcaca
     2461 tcttacattt atgatgagaa ctcctggcga atttgctccc catgctggtc gagcgttgcc
     2521 gctcccggca taacatgcgg gtccgcgcag ccactgattt ttatcggatt acccggcatc
     2581 attattcccc cggcttcaat caacatattt ctcgcctgag tttgtggcaa attaatggcc
     2641 tcagccacac ttaataacgg cgcgacgggc acaccaactt catgtattct ggctaaccaa
     2701 acttcagctg cctgtgtttt taacgtccgc tcaatatatt gtttaagaat agtctggttt
     2761 tgtacgcgta aaatattgct gctaaatctg ggatcattaa ccagttccgt aagctccagt
     2821 gcctggcata acgcagagaa aagcttgtca ttaccacaac aaatcgtaat cggcttatcc
     2881 tgagtatcga aaacatcaaa aggtgccatg taggggtggc gatttcccag gcgttgtggt
     2941 gacttccctg tcgcaatata tgccatcaga ccatgctcca gaaaactcag cgtggcatca
     3001 aacatcgcta tatcgacatg cgcccctctt tggctctttt cgcggccata aagtgcactc
     3061 actattccgc tgaataaata aacaccgccg catagatccg caagagaggt accaacacgc
     3121 actggcggag catcagggta ccctgtttcc atcattatcc cgctcattgc ctgaatgatg
     3181 gtatcgtagg caggagcatc ttttagcgga ccggtatgtc cgaaacctga cgatgaagca
     3241 tatatgaggc gcgggttgat ttcttgtaac ctttcccatg aaagccccag tttttccatt
     3301 gtacctgggc gaaaattctc agctaataca tcagcttgtt taagcatatt tataaatata
     3361 cttttatcgt gatcattctt taaatcaaga accacactct ctttaccatg attaataaaa
     3421 ctgtaataga gtgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacccg
     3481 gcggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt ccgaaaggtc
     3541 cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct ttgctttcat
     3601 tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta
     3661 tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat ccacaaggga
     3721 gccgtgacga caaaacccag tacgctcaca gccaatgacg ctgtaccagt gcgggtgtag
     3781 acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt
     3841 accatcatct gcagatgttc gctgttcaaa tgacatgcca taccaacaag aagcagcgcc
     3901 agtggcatca gaatcagctt caggaaggtg ttataagcaa tttcggcact gaactcgaat
     3961 ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca
     4021 atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccaa caccaggatc
     4081 gttgccagaa caggtgccca tacaactggc tcctttgccg cagaaattaa tgcgctcaga
     4141 ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga
     4201 atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt tgatacggaa
     4261 tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacctgca
     4321 attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac
     4381 caggagaaaa agaaacatcc gacaataacc acgagtgata caagggtcag acgagtgtcc
     4441 gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagggctgc aggaagcgcg
     4501 tagtttaata ccagtttatt aaatgcccgg gcttgatctt ctgaaaaagt ttctcgtctg
     4561 ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat
     4621 gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga gttggcaaga
     4681 ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg
     4741 ccactttcag ttcctgctgc agggtcgatg accacattaa taatggtcgg tttgcgcgac
     4801 tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg
     4861 ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc tgttggtgat
     4921 ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc attattaaaa
     4981 ataacgatcg ttaccggcag gttatatcga caaatcgttt caatttccat cccactgaaa
     5041 ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaggtcac gctagcaccg
     5101 atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag acgacgacgt
     5161 ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc ttcattaact
     5221 aaataaatat cctggttctc acgcaataca tcgcgcacag cacttaatgc attaaagtaa
     5281 tttaatggtt gggtatctgt acttaatttt tcatgcattt tttgtgcatt ttgctgcttg
     5341 tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg tttcagctct
     5401 gccagcatac cttgcatact ggatgcaata tcgccaacga ctggcacagc aatggggcgg
     5461 ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat
     5521 ccttttttac cgtgtgccag taaccaattc agtcgtgcac caacaagcat gacaacgtca
     5581 gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcgagg
     5641 atccctttcg ccatagacat tggcaggaat ggaatctggg cactttcaat aaattcacga
     5701 agctgttcgt cagcttgtga atacgccgcg cctttgccaa ggataattaa tggccgttca
     5761 gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc
     5821 gacggatttt caacttttac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc
     5881 aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac tgagacccgg
     5941 atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg
     6001 gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg ctgtaggtcg
     6061 acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgcg
     6121 ttggccaatg cggtcaaacc attgaggaat cctggcgcag acactgtcag gcagatccct
     6181 ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg ctcatgacga
     6241 aaaccaatat aacgaatgcc ttccgcctgg gcatggcgcg ccatatccgt cacaggaata
     6301 cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc
     6361 ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat
     6421 tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt
     6481 catcgtcgct ataacccagc tcctgcaata ccgcagcggt atgttcacct aatagaggcg
     6541 cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaact gtcagatatt
     6601 ttccacgcaa tggttgttcc acttcgacaa cactgccgct ttggcgtaaa gagggatcaa
     6661 gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaataag
     6721 ccactgcttc atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga
     6781 aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc caatctggtt
     6841 tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg
     6901 cgttaggatc agtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac
     6961 ccgcattacc tccgcgggga actgcatcac caaatgtgcc attcggatac tgcgggtatt
     7021 cttccagata acccaattta tcgagacgct gctggtcgcg taatttcacg cggcaaaggt
     7081 tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat
     7141 gcagcaaagc agcaagtaaa ccgatcagta aatgcattcc ggtgttgctg tcacccaacg
     7201 ctgcagcgct taccagcggc ggaccgtccc aaaaacccgt agtggatgcc gcgccacccg
     7261 ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc
     7321 ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc
     7381 ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca
     7441 gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata gaacgtttgt
     7501 tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc agctggtgac
     7561 gcgttacgtc accaacgccg gggcgttcaa ttttaatgac gtcagcgcca aaccaggcca
     7621 gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt
     7681 gaagtggagt tga
//
LOCUS       DBFIFS010000210         7663 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.210, whole genome shotgun sequence.
ACCESSION   DBFIFS010000210 DBFIFS010000000
VERSION     DBFIFS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.210"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..636
                     /locus_tag="N7A07_003960"
     CDS             <1..636
                     /locus_tag="N7A07_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478530.1"
                     /translation="VIARAGEYKNYSRDEIKYWRYTSYKGGKLPEGFTDEKFSSAIYN
                     GRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGACPSTYELYE
                     GDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGDGCKANTCGW
                     DFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
     gene            652..1062
                     /locus_tag="N7A07_003961"
     CDS             652..1062
                     /locus_tag="N7A07_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478531.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1283..2104
                     /locus_tag="N7A07_003962"
     CDS             1283..2104
                     /locus_tag="N7A07_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2478532.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2186..2665
                     /locus_tag="N7A07_003963"
     CDS             2186..2665
                     /locus_tag="N7A07_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2478533.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSSIMRIID"
     gene            2680..3156
                     /gene="yeeS"
                     /locus_tag="N7A07_003964"
     CDS             2680..3156
                     /gene="yeeS"
                     /locus_tag="N7A07_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU2478534.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3219..3440
                     /gene="yeeT"
                     /locus_tag="N7A07_003965"
     CDS             3219..3440
                     /gene="yeeT"
                     /locus_tag="N7A07_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU2478535.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3514..3882
                     /gene="cbeA"
                     /locus_tag="N7A07_003966"
     CDS             3514..3882
                     /gene="cbeA"
                     /locus_tag="N7A07_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGU2478536.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3971..4344
                     /gene="cbtA"
                     /locus_tag="N7A07_003967"
                     /pseudo
     CDS             3971..4344
                     /gene="cbtA"
                     /locus_tag="N7A07_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4341..4535
                     /gene="yeeW"
                     /locus_tag="N7A07_003968"
     CDS             4341..4535
                     /gene="yeeW"
                     /locus_tag="N7A07_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2478537.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4581..4661
                     /locus_tag="N7A07_003969"
                     /pseudo
     CDS             4581..4661
                     /locus_tag="N7A07_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4950..5096)
                     /locus_tag="N7A07_003970"
                     /pseudo
     CDS             complement(4950..5096)
                     /locus_tag="N7A07_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5150..5332
                     /locus_tag="N7A07_003971"
     CDS             5150..5332
                     /locus_tag="N7A07_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU2478538.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5433..5762)
                     /gene="tmaR"
                     /locus_tag="N7A07_003972"
     CDS             complement(5433..5762)
                     /gene="tmaR"
                     /locus_tag="N7A07_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU2478539.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5934..6992)
                     /gene="yeeA"
                     /locus_tag="N7A07_003973"
     CDS             complement(5934..6992)
                     /gene="yeeA"
                     /locus_tag="N7A07_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU2478540.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7191..>7663)
                     /gene="sbmC"
                     /locus_tag="N7A07_003974"
     CDS             complement(7191..>7663)
                     /gene="sbmC"
                     /locus_tag="N7A07_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU2478541.1"
                     /translation="NYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVPK
                     EWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVVG
                     DDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT         1987 a         1969 c         1866 g         1841 t
ORIGIN      
        1 gttattgcac gtgccgggga atataaaaat tacagccggg atgaaataaa atactggcga
       61 tatacatcat acaagggggg gaaacttccg gaaggtttca ctgatgagaa attttccagc
      121 gccatttaca acggaagaat atttacaatg aaacgtttac atacccttat gttatttctg
      181 gcggttctgt tcacgggctt taactttaac gcagaagcgg ctaccgtaaa acaagctctt
      241 agttgtaacc cggaagcctg ggctgaacaa cctggagcgt gtccatcaac gtacgagttg
      301 tacgaaggtg acgctaccta caaagccgct atcgacaaag cattaaaacc ggtcggactg
      361 agcggcatgt tcggtaaagg cggctatatg gatggccctg gtggaggtat cacgccagta
      421 aacattaacg gtacagtctg gttccagggc gacggttgca aagccaatac ctgcggctgg
      481 gactttatcg taacactcta taacccaaaa acccatgaag ttgttggcta ccgttacttt
      541 ggtttagatg acccggccta cctggtttgg ttcggtgaaa ttggcgtgca tgaattcgcg
      601 tatctggtga aaaactacgt agctgcggtt aactaattta aggagtctat catgaaaact
      661 tttatcaaaa ctttactcgt tgctgtaact attctgttct ctgtcttcgc tactgcgaaa
      721 caagtaaaac tgccaaataa catcaaatac gttaatacta cagaagcgtt ttcctgtacc
      781 gaaattgacg gtatgaattg ccagacaaag aatcagttca actataaaga taacagctat
      841 gttttcgtgc ttgaacgtgg tggtgcctgg tgctacgact acactgtctc ggtagttaac
      901 ctgaaaaccg ggaaagcaca aatgattgaa tacggggaca atcaactgtg ctcaggtagc
      961 aacaaaccgt tcttcgaaat caaaaatggc gtaccgacgg taggagtcat cgacacatcc
     1021 ggaaaacctg tcggtgtagc ccaggacaaa cttaaaatct aaggagtaaa ccatgcgttt
     1081 ctctgcacat tattaatatc aaaggtaaat acagccccag ccattgttac cggaaagccg
     1141 cttaatgcgt ggctcgccag tgaaaggcct ctcagacaat aaaccttatt catttcaccc
     1201 gtcaggccgc gtcttctccg ggagacgcgg cttttttcat ttattgcacc agtaaatctt
     1261 aaccaccgat aaggagcaaa gtatgcgatt agccagtcgt tttggttatg ctgcaaacca
     1321 gatacgccgt gaccgtccgc tgacacatga agaactgata cgccatgtac ccagtatttt
     1381 tggggaagac cggcacacct cccgcagtga acggtatgcg tacattccca ccattaccgt
     1441 cctggaaaat ctgcagcggg aaggctttca gccgttcttc gcctgccaga cccgtgtgcg
     1501 cgacccgggc cgccggggat acacaaaaca catgctgcgt ctgcggcggg ccggagagat
     1561 aaacggagaa catgtccctg aaattattct gctcaactct catgacggta cctccagcta
     1621 ccagatgctg ccgggttact tcaggttcgt ctgccagaac gggtgcgtct gtggccagtc
     1681 tctgggggaa gtgcgtgttc cacaccgggg aaatgtagtg gagaaagtta tcgaaggggc
     1741 ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag cgtgatgcca tgcagtcgct
     1801 ggtcctgccg caaccggcac gccaggcgct ggcacaggcg gcactgactt accgttatgg
     1861 tgacgaacat cagcccgtca ccaccgccga cattctgacg ccgcgacgcc gggaggatta
     1921 cggtaaggac ctgtggagcg catatcagac catccaggag aatatgctga aaggcgggat
     1981 ttccggtcgc agtgcaaaag gaaaacgtat ccatacccgg gccattcaca gcattgatac
     2041 cgacattaag ctcaatcgcg cattgtgggt gatggcagaa acgatgctgg agagcctgcg
     2101 ctgatgccgt ttccctgagt gagggaatca ttcagctttc gttcccgaaa aacacacatt
     2161 tatttgttat ttaaggagtt atctcatgaa aaccgtttct cagaataccc ccacaattta
     2221 ttcagctaca acaccagaga ataatccgcc tcagttggtt gccagcctcg tccctgatga
     2281 acagcgcatc agcttctggc cgcagcattt tggcctcatt ccacagtggg tcaccctgga
     2341 gccccgtgtc ttcggctgga tggaccgtct gtgcgaagac tactgcggtg gtatctggaa
     2401 tctgtacacc ctgaacaacg gcggggcatt tatggccccc gaaccggatg atgatgatga
     2461 cgaaacatgg gtactgttca atgccatgaa cggtaaccgc gctgaaatga gcccggaagc
     2521 cgccggtatt gccgcctgtc tgatgacgta cagccatcat gcctgtcgta cggagaatta
     2581 tgccatgacg gtccattatt accggttgcg ggattacgcc ctgcagcatc cggaaggcag
     2641 ctccattatg cgcatcattg actgaaaggg gccggaataa tgcaacagat ttcctttctg
     2701 cccggagaaa tgacgcccgg cgagcgcagc ctcattcaac gggccctgaa aaccctggac
     2761 cgccatcttc atgaacccgg tgtggccttc acctccaccc gtgcagcacg ggaatggctg
     2821 attctgaaca tggcgggact ggagcgtgaa gagttccggg tgctgtatct gaacaaccag
     2881 aatcagctga ttgccggtga aaccctcttc accggcacca tcaaccgcac ggaagtccat
     2941 ccccgggaag tgattaaacg cgccctgtac cacaatgccg ctgccgtggt actggcacac
     3001 aatcacccgt ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg
     3061 gtacaggcac tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac
     3121 caggttttct cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg
     3181 ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaattatc acccgtggtg
     3241 aagccatgcg tattcaccaa caacatccga catcccgtct ttttccgttc tgtaccggta
     3301 aataccgctg gcacggcagt gctgaagcgt ataccggtcg tgaagtgcag gatattcccg
     3361 gtgtgctggc cgtgtttgct gaacgccgta aggacagttt tggtccgtat gtccggctga
     3421 tgagcgtcac cctgaactga gtgggaattc tgatgagcag aattatcgcc actaccggac
     3481 cattcttagc cgattttctg taaggatttt atcgtgtcag acacactccc cgggacaaca
     3541 cttcccgacg acaatcacga ccgcccctgg tgggggctgc cctgcaccgt gacgccctgt
     3601 ttcggggcac gtctggtgca ggagggtaac cggttgcatt accttgccga ccgcgccggt
     3661 atcagaggcc tgttcagcga tgcagatgcg taccacccgg accaggcctt tccgctgctg
     3721 atgaaacaac tggaactcat gctcaccagc ggtgaactga atccccgcca tcagcatacc
     3781 gtcacgctgt atgcaaaagg gctgacctgc aaagccgata ccctcagcag ttgtggttac
     3841 gtttatctgg ctgtttatcc gacgcccgaa atgaaaaatt aactctccag aatagccttc
     3901 tgctacggcc tggtgttttc accacgccac ttttccattt ttatatctgc atatcaggaa
     3961 aatcttcagt atgaaaacat tacctgtatt acccgggcag gcggccagtt ctcgcccgtc
     4021 tcctgttgaa atctggcaga tactgctgtc ccgactgctg gaccagcact atggcctcac
     4081 actgaatgac acaccttttg ccgatgaacg tgtgattgag cagcatattg aggcaggcat
     4141 ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gcgctggtgc taccgaccag
     4201 ccgggattca gcgcctgtac ccgctctcag ttaataaaca gcatcgatat cctccgggct
     4261 cgcagggcga ccggcctgat gacccgcgac aattacagaa cggtaaataa cattaccctg
     4321 ggtaagtatc cggaggcgaa atgatgacac tggaagccga cagcgttaac gtacaggcgc
     4381 tggatatggg gcacattgtc gttgacattg atggtgttaa tatcactgaa ctgattaata
     4441 aggccgctga aaacggttat tcactccgcg tggtggatga ccgtgactct accgaaacac
     4501 cggcaactta tgccagccct caccagttgc tgtaagacaa tgcagtgatg cagggtatag
     4561 tgccattaac cacagaaaca gcactgttgt cctgtattaa gcaggcaaga gaagatgccc
     4621 ttaccctgcg ccatctgcag cttctgcacc agaaccggtg aatctcactc aacaggcttc
     4681 atttcccctg acgccagcct gaatacagct ggcgttttca tttataaaca gaaaggaaaa
     4741 ccgataataa tggaatggac gccccgacca tgaagcaacg gtaggatatt actgataaac
     4801 cttccagtgg aggtgcgtta tggccgtttt aacgattagc attgacctga caaaaaatga
     4861 attccaaatc cacggtctgg gtaggaacag aaaaatctaa gctcagaaaa cggattaaag
     4921 taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagaactac gttccatggc
     4981 aggagttcgt caacacggtt ggagggccat tccggcagta tgctcagaat atggcgcaga
     5041 tacgcttccg gatcgatacc gttcagacgg caggtgcctg cttttccggc aacaagcagg
     5101 aatctcagcg ctgaagcagg aattagaggt aaaaacgccc taccgggcca tgaatcaccc
     5161 ggtcattgga gtagtaacca aagcagattt agccagcatg gaacaaatat ctttggtgaa
     5221 atgttggtta cgggaagctg gagcgcacaa cgtgttagta accagtgcgg ttaataacaa
     5281 tggagttaca gaactttttg ccttgctgca tacagaagaa ggctgccgct aatttcgata
     5341 ggttctcaga tgagaatgat gacggtgata cacagataag aatcaatgag caatgagggt
     5401 tgccgggcaa ccctcattga ataaaacagg aattacttcg cttcgccgtt tttcatttcg
     5461 cccatagctt tcagcttttt ggagatatcg cggcgttctt tggagagctc ggcatttttg
     5521 atgatgtaat cgtcaacgcg atcttcatag tcacctttca tgctggcgat gatgccctgg
     5581 attgcttcaa cgctcatccc tggcttgatg taatcgctca ggttgtccag caacaggacg
     5641 cgcttctggt tgtcacggat ctttttctca acgtcctgaa tttcacgttg cagtttgttc
     5701 ttacgacgga acagacgaac aaattccagt acgtcctgga atgaaggctt ggtagtttcc
     5761 atttttatac ccctgataat gtgagagtcg gattcgttta agcaaccgct attcgttagg
     5821 gccaacatta ctcatagctg ccgcagatga caatgctttt atcccttatc actatcatac
     5881 cctttatcct tgctgaatcg aagcagcagc aagatgattc tgaagttcag gaattatttg
     5941 cgcaaggccc ggcaaatcag actcgatagc aactcaagct gatgtgccgt ttccatgttc
     6001 agccacacat aaccatagat tggtgtttcc acaaccttca ggtcatggtg gttattcagc
     6061 aactgacgca gctcttccac agcatcgttc aatttttccg tattggcaaa aaccggctgc
     6121 ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat catgtgctgg
     6181 gtatcacgaa gtttttgcgc gttcaataac acgaaatggc tggggcgcgt ggcccagtat
     6241 gcattgattt gcaactccag catacaaacc agattgcggt taatggtctg gataccttca
     6301 tatatcgatt ttggaatacg agtttctttg ctggcgggcg caatcagccc acgcattttc
     6361 acggcatcgg tcaggagttt ttgtagatgg ctttccagac gtgggcgttc gagtaagttc
     6421 ggtgagaatg cagattgata gacccgatta tactcggtca gacttttcgc cagttgaatg
     6481 cgccagtgga tgaacgcccg ttgtggccag ataccggtaa acaacattgc cagtaaagag
     6541 ccgaggatca catcgccgct tcgccataac gccgtatcaa tttcacctgt cggggaaccc
     6601 acaacaattg ccagcgtcac cccaatcaat aatccttgat acggtttctt gcccagcgcc
     6661 agccaaccgc aaagaaacat cgccgccgcg caccagacta acatcagcgg taacgaaatt
     6721 aactccagtt gcagagcgat aagacctaaa atcgacccca acaccgtacc gccaatacgt
     6781 tcgaaggcac gagggacaac gttcccccag aacgagattg gccccataat caccaccatg
     6841 gtgaccagcg gccaggtgcc ttccgggata gtaaacaggc ggataatgag aaaagtgagc
     6901 aggaatgcca gcgcgacccg agtaccatgc acaatgcggt aatggcggta tacccggatt
     6961 tcaaacgggc ttaatgactt attggcacgc acgcagaaac tccgttaatg aagattaaaa
     7021 gcgctcataa cacatctgtc ggtcagaagg cgcgcctctg aattatccga cggacaaacc
     7081 gggaacagtg taatttcccg gcaggagcga aaaaattgaa aggcgcatct tactcttttc
     7141 gctttcaaaa aaagagtggt cttcgcgtca acacaccgcc ctgagatgaa ttagtgatgt
     7201 tttggctgca ccgcaacata catttcgata tcccagtacc catcttccgc gccattgttc
     7261 aagtaaacct cgaagcaggg ctttggtaac atttcataag cactgtcctg caagaggcta
     7321 ttaaagaact gataccaggg tttagcaaaa tcatcaccga ctacacgagc caccgccacc
     7381 gcatattgac cacctgaaat ttctgtcaga atgacgccct cactgttttc ggggagcgta
     7441 aagttattcg gtaccgtcac gacggtgtcg cagcgtaatt tttcggcggg tgtttcatcc
     7501 ggattgtcgt aatagacagc aacccactcc ttcggcacaa tatttttgct atctacccac
     7561 atcatcaact gctcaaaacc tttctttacc gtctgttccc acgggccaac gagatggaaa
     7621 cctgcaacgg tacgtttatc ttcctgctta atctcgtagt tca
//
LOCUS       DBFIFS010000211         7579 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.211, whole genome shotgun sequence.
ACCESSION   DBFIFS010000211 DBFIFS010000000
VERSION     DBFIFS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.211"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1274
                     /gene="ydfJ"
                     /locus_tag="N7A07_003975"
     CDS             <1..1274
                     /gene="ydfJ"
                     /locus_tag="N7A07_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HGU2478542.1"
                     /translation="LYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFI
                     FGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGA
                     EISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWR
                     IPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLR
                     FGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRI
                     PYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFG
                     CKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKM
                     PEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            1363..2823
                     /locus_tag="N7A07_003976"
     CDS             1363..2823
                     /locus_tag="N7A07_003976"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU2478543.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2859..3062)
                     /gene="ydfZ"
                     /locus_tag="N7A07_003977"
     CDS             complement(2859..3062)
                     /gene="ydfZ"
                     /locus_tag="N7A07_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU2478544.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3239..3925)
                     /gene="rspR"
                     /locus_tag="N7A07_003978"
                     /gene_synonym="ydfH"
     CDS             complement(3239..3925)
                     /gene="rspR"
                     /locus_tag="N7A07_003978"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU2478545.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4014..4760)
                     /gene="ydfG"
                     /locus_tag="N7A07_003979"
     CDS             complement(4014..4760)
                     /gene="ydfG"
                     /locus_tag="N7A07_003979"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU2478546.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4897..6942
                     /gene="dcp"
                     /locus_tag="N7A07_003980"
     CDS             4897..6942
                     /gene="dcp"
                     /locus_tag="N7A07_003980"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU2478547.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6987..7505)
                     /locus_tag="N7A07_003981"
     CDS             complement(6987..7505)
                     /locus_tag="N7A07_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU2478548.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1869 a         1784 c         1928 g         1998 t
ORIGIN      
        1 agttatattc gctcggcgca gcgttagtgt ttcatgaaat attttttcct gaatcatcaa
       61 cggcaatggc gttaattctg gcaatgggaa cctacggtgc aggttatgtg gcgcgtattg
      121 tcggagcatt tattttcggc aaaatgggcg acagaatagg gcgtaaaaaa gtgctcttta
      181 ttaccatcac catgatgggg atctgtacca ccttaattgg agtgttaccg acctatgcac
      241 agattggtgt ttttgccccc atcttgctgg tgacgctgcg tattattcag ggattgggtg
      301 caggtgcgga aatttccggt gccggtacga tgctggcgga atatgcgcca aaaggtaagc
      361 gcggaattat ctcctcattt gtggctatgg gaactaactg cggaaccttg agcgcaacgg
      421 caatctgggc ctttatgttc ttcattctca gtaaagagga actgctggcg tggggatggc
      481 gtataccgtt cctggcgagc gttgtcgtga tggtctttgc tatctggttg cgtatgaatc
      541 tgaaagaaag cccggtcttt gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg
      601 cacctgctgg tagcatattc cagagcaaat ccttctggct ggcaacaggg ctgcgttttg
      661 gtcaggcagg taactccggg ttaattcaga ctttccttgc aggctattta gtacagacgt
      721 tattgtttaa caaagcaatt ccaacagatg cattgatgat cagttcgatt cccggcttta
      781 tgaccattcc gttccttggt tggttatccg ataaaattgg tcgccggatc ccgtatatta
      841 ttatgaatac ctccgcgatt gtgctggcat ggccaatgct ttctatcatc gtagataaaa
      901 gctatgcccc gagcaccatt atggttgcac tgattgtgat tcataactgt gcggtgctgg
      961 gattatttgc tctggaaaac attaccatgg cagaaatgtt cggctgtaaa aaccgcttta
     1021 cccggatggc tatttctaaa gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc
     1081 tggcgggtat tttctgcacc atgacggaat cctggtatcc gatcgccatt atgatcatgg
     1141 catattcagt gattggttta atctctgcgc tgaaaatgcc agaagtgaaa gaccgtgatt
     1201 taagtgcgct ggaagacgct gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt
     1261 ccagaagtct gtaaatcctc aatcccttct cttaccggag aggggatttt tattcatata
     1321 acaaaacatg tagcttgcca tatttaaatt taaggaattg ttatgggaaa taatttgtta
     1381 tcagcaaaag cgacactccc tgtttatgat cgtaataacc tggccccaag aattgttcat
     1441 ttaggctttg gtgcatttca ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca
     1501 gaacatttca gtgactgggg atattatgag gtcaacttaa tcggcggcga acagcaaatt
     1561 gccgatttac atcagcaaga taatctttat accgttgcgg aaatgtcggc cgatgcgtgg
     1621 acggctcgcg tcgttggcgt cgttaaaaaa gccttgcacg tacagataga tggcttagaa
     1681 accgtgttgg ctgcgatgtg tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa
     1741 aaagggtatt tccactctcc ggcgaccgga cagttaatgc tcgatcaccc gatggtcgct
     1801 gccgacgtgc aaaatcccca ccagccgaaa acagcaacag gggtgattgt tgaggcgctg
     1861 gctcgccgta aagcggcagg acttcccgca tttaccgtca tgtcatgtga caacatgcca
     1921 gaaaacggtc atgttatgcg tgacgttgtc acttcctacg cacaagccgt tgatgtaaaa
     1981 ctagcacaat ggatcgaaga taacgtgact ttcccatcaa caatggtgga ccgtattgtg
     2041 cccgcagtga cagaggatac gctggcgaaa atcgaacagc ttaccggtgt gcgcgatcct
     2101 gctggcgttg cctgtgaacc tttccgccag tgggtaatcg aagataactt tgttgccgga
     2161 cgtccggaat gggaaaaagc gggagccgaa ctggttagtg atgtgctgcc ttatgaagag
     2221 atgaagttgc gcatgctcaa cggcagtcat tcattcctgg cgtatctggg ttatcttgcc
     2281 ggatatcagc acattaatga ctgtatggaa gatgaacatt atcgtcatgc ggcgtatgcc
     2341 ttgatgttgc aggaacaagc gccgacgctg aaagtgcagg gcgttgattt gcaagattac
     2401 gctaaccgat taattgcacg ctatagcaac ccggcgttac gtcatcgaac ctggcagatt
     2461 gcgatggatg gcagccagaa attgccacag cggatgttgg attctgttcg ctggcatctg
     2521 gcgcatgaca gcaagttcga tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc
     2581 ggtggtgttg atgaacaggg aaatccgata gaaatcagtg acccactgtt acctgttatt
     2641 cagaaggctg tacaaagtag tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt
     2701 aaggcgattt ttggtggtga tttgccagac aatagcttgt ttactgcaaa agtgacggaa
     2761 gcgtacttgt ctttattagc gcatggtgcg aaagcgaccg tggcgaaata ttccgtgaag
     2821 taaaaaaagc aatgccgcca tcggcggcat tcacacgctt agttcatgcc gagacgaatc
     2881 aggtccagtg gtgccagttt ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg
     2941 atttgctcag cggtcagacc ttcagtatca atcgacagga tcttgccagt gtgaccggtg
     3001 ccgctaacca taacacggct gccagtggtg attgcgttac ggttacgatc gtaggtcgtc
     3061 ataggtgttt tctcctttct gatttacagt cgaagcccac gtaacgggcg caatataaat
     3121 actcctgtcg agtgaaatat tttttgtttt tgatcaagct cacatctttt ttgatgcatg
     3181 catgagccat aaggcggggg aaaagactct caccccggat gggatgagag gaaatgaatt
     3241 actcttcgct aaaccagtcg ctattttcct ggcggatctg gcgcacggat tcgctgattt
     3301 cctgcaaatg ttgcgtcatt gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag
     3361 agaatatatc aagatgctgg cgtaacagca tttctggtgg agaaacatgg tcgaaactca
     3421 tatagcgcac gcgatcaacg gtcgctttca ggttctcaat ggtatcccac gccagttgac
     3481 agtcggcaat ctgcgtcagg agttgatgga agttgtcatc aagttcaaaa aaatcatcca
     3541 gttgcttgcg ctcaatggca atgcgttgct ggtgaagatt ttgttccagt tggtagcact
     3601 ggctttcggt aatcatactc gccgcccgac gcgccaccgc gcactcaatg gcctgacgga
     3661 taaaactgcc gttgcgcacc tgggccatgg aaattttgtt gacgtagctg ccacgctgcg
     3721 gacgaatttg aatcaggccg ttttccgcca gtttaataaa ggcttcacga accggctggc
     3781 gtgacacatt gaaacgaaca gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca
     3841 ggcaatggac aatgtcgcga cgaagaatac gataaatctg ctgattgaca ggctgtgtgg
     3901 gattaagttg cgtttcgacg gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt
     3961 ctaccacttt tttgtgacaa gcaataaccc ggcagttaca ccgggtataa aaattactga
     4021 cggtggacat tcagcccggc atagctttgg gtaaccggca tcatttccag ggtattgata
     4081 ttgacgtgag caggcagcgt tgacacccac cagacggctt cgctgacatc ttctggcgtt
     4141 aatgcaacgg tattttgata ggttttttcc gctttaccgt catcgccttt aaagcggaca
     4201 ttggaaaact cggtgccacc caccagaccc ggttcgatgt cggtgacgcg caccgccgta
     4261 ccatgcaggt ccgtacgcag attcaggcta aactgacgaa caaacgcttt cgtcgcaccg
     4321 taaacgttac caccggcata cggccagcta cctgccgttg agccaatgtt aataatatga
     4381 ccatgattac gttcaaccat acccggtaag acggcgcgcg tcatatatac caggcctttg
     4441 ttgttggtat caatcatcgt ttcccagtct tcaatgctgg ctttatgcgc aggctccatg
     4501 cccaacgcca gaccggcatt atttaccagg atatcaatat tgcaccactc ggcaggaagc
     4561 gatgccagca tctcttcaat agcggcgcgg ttgcgaacgt ccagttgggc gatgtacaga
     4621 ttatctccca gttcgtcttt taactcctgc aaccgttcct ggcgacggcc agtggcgata
     4681 actttatgcc cttgttgaat aaaacgacga gtaatgcatt caccaaaacc tgccgttgct
     4741 ccagttacta aaacgatcat ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc
     4801 acgtgatgct taagagcggg taatactggt ttgcaagatt gcactccgtt atcagtaagc
     4861 ctacgctatg gaacacctcg cgttcaggag aagaaaatga caacaattaa tcctttcctt
     4921 gtgcaaagca cactgccgta tctggctcct cattttgatc aaattgccaa tcatcactat
     4981 cgcccggcat tcgatgaggg aatacagcaa aagcgggcag aaattgctgc catcgcgctt
     5041 aacccgcaaa cacctgattt caacaatact attctggcac tggaacaaag cggagaatta
     5101 cttacccgcg ttaccagcgt cttttttgcg atgactgcgg cgcataccaa tgatgaatta
     5161 cagcgtcttg acgagcagtt ttccgctgaa ctggcggaac tggctaatga tatctatctg
     5221 aacggtgaat tattcgcgcg ggtagatgct gtctggcaac gccgtgaatc tctggggctt
     5281 gatagtgaat ccatccgcct ggtggaggtg attcatcaac gttttgtcct agccggagcc
     5341 aaacttgcac aagctgataa agcaaaatta aaagtactga atacagaagc tgcgacactg
     5401 accagtcagt ttaaccagcg gttactggca gcaaataaat ccggcggtct ggttgtgaac
     5461 gatatcgcgc agctggcagg aatgagtgag caagagattg cgctggcggc agaggcggct
     5521 cgcgagaaag gtctggataa caaatggctg attccgctgc tgaataccac ccaacaaccg
     5581 gcgcttgccg aaatgcgcga tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg
     5641 cgagcggaaa aaaatgatgc caatgatacc cgcgctatca ttcaacgtct ggtggagatc
     5701 cgtgcacaac aggctacatt actcggtttt cctcattatg ccgcatggaa aatcgccgat
     5761 cagatggcaa aaacacctga agcagcactc aactttatgc gggaaattgt tccagcggcg
     5821 cgtcaacgtg cgagcgatga attagcctcc atacaggcgg ttattgataa gcagcagggc
     5881 gggtttagcg cgcagccgtg ggactgggca ttttatgccg aacaggtccg gcgagagaaa
     5941 tttgatcttg atgaggcgca gctcaagcca tattttgaat taaacacggt gttgaatgaa
     6001 ggtgtattct ggaccgcgaa tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt
     6061 cctgtctacc atcctgacgt tcgggtgtgg gaaatttttg atcataatgg cgtggggctg
     6121 gcgttatttt acggtgattt cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc
     6181 aattttgttg agcaatcaac gcttaatgaa acgcatccgg taatttataa cgtctgcaat
     6241 tatcagaaac ccgctgccgg tgagcctgcg ttgttactct gggatgatgt cataacctta
     6301 ttccatgaat ttggtcatac gctgcacggc ctttttgccc gccagcgtta tgccacgctc
     6361 tccggcacca acacgccgcg tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg
     6421 gcaacgcatc cgcaggtatt cgctcgctac gcccggcatt atcagagcgg ggcagcaatg
     6481 cctgacgaac tgcaacagaa aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg
     6541 agcgaactgc ttagcgccgc acttctcgat atgcgctggc attgcctgga agaaaacgaa
     6601 gcaatgcagg atgtcgatga ttttgaattg cgggcgctgg tggcagaaaa tatggatctt
     6661 cctgctatac cgccacgcta tcgcagcagt tattttgccc atatttttgg tggcggatat
     6721 gccgcaggtt attacgctta tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg
     6781 tttgttgagc agggcggatt aacgcgtgaa aatgggctgc gttttcgcga ggcgatcctt
     6841 tccagaggta acagcgaaga tctggaacgc ctgtatcgac aatggcgcgg taaggctcct
     6901 cagattatgc cgatgctgca acatcgtggc ttgaacatat aaacaccacc aatggctgtc
     6961 ctggagatat cgccaggcca gcctggttat agcagtaact gcagcagctg ggcgagggca
     7021 aacctcaccg ctaaagctaa taccgtttcg cagtcgccag caaaatgcat aaccgcagtg
     7081 tagttctggc ctttaatatg ccacgcgaac cagacggtac ccgctggcgt accatcttca
     7141 ccgccctccg gtccgccgta gccggtaatg gcaatactga catcagcatc cgcacgctct
     7201 atggcaccgg ttgccatttc tgctgccact ttctcactca ccgcagaata tcgttcaaga
     7261 gattgctggc ttacgctgag gattttcatc tttgcctgat cggtgaaagt aacaaagcct
     7321 gcaccgtaaa atttgggtgt atcttcagct gcacacaggg cgctagccaa cttaccgccg
     7381 gtgcatgatt cggcggtagt taagcgtaat tgatccgcga taagtctttg actcaacgcg
     7441 gatgtcaggt tttcaatagt gtcggtatct gcaagttgta caattttatt tctattaatg
     7501 ttcactgttt tttgtccacg cttttatatg taaaggtgat ttgctttttc tgtatgctcg
     7561 tccaatataa taattttca
//
LOCUS       DBFIFS010000212         7521 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.212, whole genome shotgun sequence.
ACCESSION   DBFIFS010000212 DBFIFS010000000
VERSION     DBFIFS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.212"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..4782)
                     /locus_tag="N7A07_003982"
     CDS             complement(<1..4782)
                     /locus_tag="N7A07_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2478549.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPAPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDITLNKT"
     gene            complement(4802..5263)
                     /locus_tag="N7A07_003983"
     CDS             complement(4802..5263)
                     /locus_tag="N7A07_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU2478550.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITRDPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5291..7192)
                     /locus_tag="N7A07_003984"
     CDS             complement(5291..7192)
                     /locus_tag="N7A07_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2478551.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1443 a         2250 c         2001 g         1827 t
ORIGIN      
        1 tgttttatta agtgttatat caaatccttc aataactgga tgtaatccat attggtttgt
       61 aggagatgtg atatcaataa taccggcatc tagacgtttg gaaccacctg tataatgagt
      121 gccggatgtc cacttaccat ttactttaaa tggtccattc gttgtcgcag gaactcgctc
      181 tccatttttt atggctggtt gaatctcaac tctaaattta ctacctgtta attctagtct
      241 tttatttatc atgtttatgt atttattgaa tgcagcctct gattttatcc aatcaccatt
      301 agcatattta agtgctctat tccctaatga gttcaaaaca tcagatatgt tgctttgctc
      361 actcccccat ttcgtccagc gggctaactg acatatagcc agccccaaag gatcgatata
      421 cccacaagga ttcggcacat accgataagt ctgctcccct ccctgcaacc ccagcggatc
      481 actcaccagg tacatcccgg tttccggctc gtagtaccgg aaccggttat agcacagccc
      541 cgattctgca tcctgccact gtcccgcata cagcaggccg gggtccgctt ccgggtcccg
      601 ctccccgcgc ggggccgggt agtctgtgtc tgcgggcagg ctgagtgcca gcccccacag
      661 gctggtctgc cccggccgcc agacggtttt accttcactg ttaaagagca tcagcgggcg
      721 gcccgtcagg tcgctcaccg catgattcgt tcgcgtcacc cactgcgggg ccacggacgg
      781 atgcggctgt cgtacccggc tgaactgctg gcttatcagc tcaaagccgt taaacaccag
      841 gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa tactgtcgcc
      901 gtcccacagg tacgtaaaac ggattttctt ccggtcgcag cgtttttccg tccgtctgcc
      961 gctggcgtcg tggcggtatt cccactgctc cccctgtgcg ctgcgatacc cggtcagctg
     1021 gtcccggctg tcccaccgga accgctctgt ttctgggcgg tagccgtcac ggtgttttgt
     1081 gcggctgacc atccggcctg cggcgtcata gtcatactgt gtgtttccgg cctgtttcag
     1141 ccggtggcct gcggcatact ggtcagtctc tccgcttatc cggtgtctgc ccgcagactg
     1201 cgccttcagg tagccgcagc tgtcatagcc ataagtctcg tcgccttcgc catagcccag
     1261 cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtcaagcc ggtactcccg
     1321 gtgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gatacgttac ccgcgctgtc
     1381 gtaacggtat tcccgtagca gtgaagggag cagtccgccc catgtggctt ccgccgggaa
     1441 aagtccggcg cgctggctga caggctgacc ggctgcattc cagccctgtt cacaggcaaa
     1501 accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga acgtcagcgt
     1561 ctcatccccc ggcaggataa cctccgtcag ttctccggcg gcgttgtagc ggtatgtggt
     1621 tttcagcagg gatccgtcgg ggtgtctgtc gcgttcatct tccggtggca ggatacagcg
     1681 gatgacggtg tgttcgtcgg ggtagtggta ttcaatggcc cgtcggccct gagtggtgac
     1741 attcacccgc ccgcgttcat catagccggt gcgctgtgat gtgccgtccg ggctgaagat
     1801 acccgtcagc ctgccggatt tgtcatacgc atactgcgtt ttgccttccg gagtgatgac
     1861 ttctgccagg cggcctgcgg catcccggct gtagaggatg gtttcctcca ggccgttgac
     1921 cctggcggta cacaggccat cagcgtcata acggtaacga cagaccacgc cgccccagtc
     1981 acgttcttcc gtcacccggc cacagttgtc cagaatataa agataagact cgccctgcgg
     2041 gttaatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc agcggtggcc
     2101 ttcaccgtcc gttcgcgcca ccggcaaatc aaacgggccg tactccattg tccagacaac
     2161 gccgttaccg tccgtgtacg ccagcaggtt gccgtggcgg tcgtagcgga atccttccgc
     2221 gccggttcct gcccgctcaa ggccactcag tttatggtgc tgctgccact gcaaccgcac
     2281 tgactgaccg ccggggccgc gcatcttcac cggcaggtcg tgaccgctgt attcggtgtg
     2341 gtgttcgcgg cccatcacgt cggtgagtgt ttccggccag ttgtggtggt cgtactctat
     2401 gcccagacgc cgttcatcct ggcggaatat ccccgacagc agcccctgag gggtgtacgc
     2461 cagccgggtc tcttcgccct gtggcgatac cagactcacc ggcaggccgc gttcatcaca
     2521 ctgctgcgtc cacgtctgcc cggcaggggc ggtgatgttg ctgagcaggc cgcgctcatc
     2581 atagcagtac tgccaatccg cgccgtctgc cgccgtgtaa cggcttaact gaccgtgtgc
     2641 ggcgttgtat tcaaacttct ccacccgccc ggcaggggag tggcgggcca gcaggtgatg
     2701 gaattcatcc cattcatacg tggtggtgct gccgtccggc gcttcttccc gcagaatatt
     2761 gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc
     2821 gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacgg tcacgcaccg
     2881 gccctgtgca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc agcggatgat
     2941 ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat cctgttcgta
     3001 ggtcgccagc gtctgtatgc cgttatccgt gcggtgaatg gctttcaggt agccgctggc
     3061 gtggtacgtc agtttaagcc ggatcccgtc ggtgtgactg acacccgtga tccggtactg
     3121 ggtgtcgcgc tcaaacgtga tgcggttgct ggcggtgtcg ctcatcccgc ccagcatgta
     3181 catccggtct gcggggctgt gtgccaggcg gtcatcgctg gtatcgtcag tcaccgggtg
     3241 gcgcagacgc attcccggaa aggcgtcata aaagaagcgg ctgctcagtg catccctgtc
     3301 gaacagctcc agataatcat cacggcggcg cagaatgtac tgggcgtgat acgggttaac
     3361 cgccgtatca ctttcaccgt cgaaggcgaa gttcagcgtg gctcccaggc tgatgacatc
     3421 cacccggttt ccctgttcac gcacccaggc gtattcgctc caggagtcgc tccagcccac
     3481 gcccgtcagg ccatgcagcg gcagtacaga actgtggaaa cgggtgaagg agagggggag
     3541 tgtctggccc agcgtaaagt cggtacgttc gtcgcagtat gcgccggtta ccggatcgac
     3601 aggatctgtt acccatctga tgactttatt ggcaatactt tttaacgaat tacctgtttt
     3661 ccccagcaac gcgccgagct ttgcgcccag cagcttaccg cttttcccca gcaggtttcc
     3721 cagtttgccc agtttcgcca gattccccag ttttcccagc agtcctgctg gcggcagaag
     3781 atagctggct atcgttattc ctcccaggat cattctctgc catggcggga actccggctg
     3841 gatatccaga taggtgacct gctcaccccc aataaagacg tccggcgaac cttccacgat
     3901 tgccgcgctg cactccagtt tgtcgccctt gcgcgcggca ggctgattgt tgataaagac
     3961 gttgctgctg ccctgggcta tcctcggtgg tttgttctcc ttgtcgcaga gcgccgtgtc
     4021 ctcaccggct tccgggaaat ccacatcttt tttgatttcg ccacgcccgc ccaccatggt
     4081 ggcaaactcc attaataccg gaccggcggg aaacagaagc gatgcgccgg cgctgacgcg
     4141 atccataaaa ccggcattgc tgtccgtcag ggtgataaca gcgttccccc aggccttcgc
     4201 cagtggtact gctttttccg ctgccgattc aagcaggtca gtggcaatat caccccatga
     4261 gggttccccc tgtgggactt caggtattgc tccaggtggg ggaggagggg gaagggtgac
     4321 ggctgcacgg gctgctggct ttccgttaat ttttacattt ttagaaccgg tattgatggc
     4381 gccgtccgtg tcacaaaagc tgtttatcca tgattcaagc tggttctgtt tctcctccac
     4441 ataatcactc gccttatagc tggcatacat cagcgcaggc accagaacca gcgcataagg
     4501 gccggtggca caggcggcca taatcaggca accttctgct atggtcacta tcgcccccag
     4561 cacggcccct gccagcgccc ccaaaaaact cttgtgctga atttcatcgc ccagcctggc
     4621 ggcggggggg ccagactttt ttgatgccaa catctgtgca atggctttgt cgttggctgc
     4681 ccgtttcatc tggctggcgg ccatcgcccg ctgcgacagc atccgcattg ccagcgtacc
     4741 gcctgcggtc gctccctgtg gcccgcctgg tccttcactc atgttttatc cctcgtttat
     4801 gtcagcgcgg ctggtccttt tggatgtcat tctccggtgg caacggggtg aagctcttta
     4861 tcacctccag cagcatcgtc ttttgactgt tcttcaggga taaggaggtg ctgaaagtga
     4921 aggtcagcag cgtatctcca cgccgctgga atacggctat ctggctaatc cgcccctgat
     4981 tcccttcatc ttccggaccg ttccaggaat aatccagcag ccaggccggg gcctctgcca
     5041 gagtgatttc ccggcaggca tgttcggtgt acccatcaag tttgccctgg tacagcgcca
     5101 gcgtttcctg gtacagctct ggtgtcaggg ttccctgtga aacggggtcg cgggtaatga
     5161 ccaggttgat gccactgtta tccgtggata ccaggacctg catcgactga tcctgccatt
     5221 gcgatggcag ttgaatatga ccttcattaa aagtgaactg cataaaatac tccctgttaa
     5281 tctgagtgaa tcagttgatg aaaacagaac cgcccacgat ggtgatgcca tccttcgaca
     5341 gcctgattac cgttgattcc ccgacctgta gctgaatgcc atccggcgtc agcgcaattt
     5401 tgctttcctc cacggccagc ataatttcct taattgccgt ataagcctgt ataccgttgc
     5461 cttcgccacc attgtttaca gcgtcaacat gcaccgaacc ggcaatcgtg cctctgtcct
     5521 gaaagacatc ctcagataaa gaccctttca gtactccggt gatcttgtgg cctttttcca
     5581 ccgtaagaag gcggttacct tcctggatcg tgaccgtctg gtcatttttt acggtttcgc
     5641 gatgatcgtg ttttacggtg gtggtacggt cgttgagcac ttcggtatcc atgttcttct
     5701 gcgcgtggat gtagacctgt tctctccctg tcgcatcatc aaacttcagc tcattaaacc
     5761 cgctgcccat atacgttttg gaacggatgg tcatctgcgt cttcgtcccc ggcaggctgc
     5821 cgggggtgcg gttttcctgg tggtaggtgc gccccataat gatcggctgg tccggatcgc
     5881 cgttgaggaa gtccactatc acctcctgac ccacacgcgg tatcgccagg ttgccaaaac
     5941 cggtgcctgc ccacgcctgt gccacacgga tccagcacga gctgtcctgg tctgccgggt
     6001 tataacggtc ccagttgaat ttcacccgca ctcggccatg ttcatcacag aagatttcct
     6061 caccctccgg ccccgtcacg acggcgctct gcggaccgtc caccagcggt tttagccgcg
     6121 gctggggtcg ccatgtccgg tctgccggga tcaccgcaaa atggttctcc agcgccgtcc
     6181 ctgctccccg ccttcccggc accgcctgcg gctgctcgcc gtgcagttca cttgccacca
     6241 cctgccattc ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg
     6301 gccatatctc cggcgagcga ctcattcccc gcgcggtttc tgcattgttt cgccagccct
     6361 ccatctgcca gcgggcaaag ttctgcccgt gggcgccctt gaaacgtccg gggtagtcat
     6421 acacctcata ctgcgtgcgc tggtaatcct ggtgctggcc ttcctgatca aaacgtcctg
     6481 cccagccggg gcgtttaaag gtgtagtctt tggtcaccac ggaagaaggg cggatttgtg
     6541 cgctgtagcg aaactggctg atgcagaggg tgctcacctc ggtacgggtg ttcgggttcc
     6601 aggggatttc aaaagattcg ggcagatggc ggaccgtgtc gcacagcacc aggctctggt
     6661 cggtactttt gtaagcatgc tcctcataaa agaagatgcc ttcctccgcc gccatccggc
     6721 acaggaaatc gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa ggatgcggtt
     6781 cactgaacag cggactccac tcggtcaccc cgttttcctg caatatcgtg ccgaggatgc
     6841 ttttgatgtc ctcgttctgg aagatacgga agttctggcg cagaccggca cgccacagcg
     6901 gcgggtgcac cttcatgctg tacagcatct ggtttttgtc gttctccccc agttcaaacc
     6961 aggtcaccac acctttcacc cggcgctgta catcatcacc ctgccatatt gtcaggtagg
     7021 ccattttgtc cagcacctgc gcaaattcaa gggagagaaa ctgctggctg accaaggaga
     7081 gatcgaggga aaagagcgaa gagagtgact gggtgagatg aaaggaaacc accgcaaaag
     7141 cgtccggtgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac atagcaacct
     7201 ccggtaaaat gaataataat ccgcaccgta caactgcgga taatgagata agcgggacga
     7261 cttaagacgt tattagggaa ggaacaaaaa atgctatgat tatgtgagga ttttgtttaa
     7321 atattacttt acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa gctcaactta
     7381 ttgtttcctg acgttattca ggacaggtgc gcacatcaca gcgagtaaca aaaacgcaaa
     7441 gttaaaccaa tattcataat atgtttttat tcacaactaa ataacccaat taacatcatt
     7501 aaaatgaatg attgcttaaa a
//
LOCUS       DBFIFS010000213         7477 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.213, whole genome shotgun sequence.
ACCESSION   DBFIFS010000213 DBFIFS010000000
VERSION     DBFIFS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.213"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            64..1461
                     /gene="ulaA"
                     /locus_tag="N7A07_003985"
     CDS             64..1461
                     /gene="ulaA"
                     /locus_tag="N7A07_003985"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU2478552.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1477..1782
                     /gene="ulaB"
                     /locus_tag="N7A07_003986"
     CDS             1477..1782
                     /gene="ulaB"
                     /locus_tag="N7A07_003986"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU2478553.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1792..2256
                     /gene="ulaC"
                     /locus_tag="N7A07_003987"
     CDS             1792..2256
                     /gene="ulaC"
                     /locus_tag="N7A07_003987"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU2478554.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2270..2920
                     /gene="ulaD"
                     /locus_tag="N7A07_003988"
     CDS             2270..2920
                     /gene="ulaD"
                     /locus_tag="N7A07_003988"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU2478555.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2930..3784
                     /gene="ulaE"
                     /locus_tag="N7A07_003989"
     CDS             2930..3784
                     /gene="ulaE"
                     /locus_tag="N7A07_003989"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU2478556.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAVEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3784..4470
                     /gene="ulaF"
                     /locus_tag="N7A07_003990"
     CDS             3784..4470
                     /gene="ulaF"
                     /locus_tag="N7A07_003990"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU2478557.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4599..4874)
                     /gene="yjfY"
                     /locus_tag="N7A07_003991"
     CDS             complement(4599..4874)
                     /gene="yjfY"
                     /locus_tag="N7A07_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU2478558.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5201..5596
                     /gene="rpsF"
                     /locus_tag="N7A07_003992"
     CDS             5201..5596
                     /gene="rpsF"
                     /locus_tag="N7A07_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU2478559.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5603..5917
                     /gene="priB"
                     /locus_tag="N7A07_003993"
     CDS             5603..5917
                     /gene="priB"
                     /locus_tag="N7A07_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU2478560.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5922..6149
                     /gene="rpsR"
                     /locus_tag="N7A07_003994"
     CDS             5922..6149
                     /gene="rpsR"
                     /locus_tag="N7A07_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU2478561.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6191..6640
                     /gene="rplI"
                     /locus_tag="N7A07_003995"
     CDS             6191..6640
                     /gene="rplI"
                     /locus_tag="N7A07_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU2478562.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6711..>7477)
                     /locus_tag="N7A07_003996"
     CDS             complement(6711..>7477)
                     /locus_tag="N7A07_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU2478563.1"
                     /translation="PAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDHNCDGRRTVEI
                     HKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLT
                     AELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSEL
                     PTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSSAAIYSGLGFE
                     RVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1784 a         1835 c         2042 g         1816 t
ORIGIN      
        1 gagtgagtgc acaacattcc gggtgtgtgg aatacccggt tacctcttct tcaggagatc
       61 gttatggaga tcctctacaa catctttacc gtatttttta accaggtcat gaccaatgcc
      121 ccgttgttgc tgggtattgt gacctgtctg ggctacatcc tactgcgcaa aagtgtcagc
      181 gttattatta aaggcacgat taaaaccata attggtttca tgttgttgca ggcagggtcc
      241 ggcatcctca ccagcacctt caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt
      301 aacggcgcaa tttccgatac ctacgcttca atgatggcaa ccatcgaccg catgggcgat
      361 gcctatagct gggtgggtta cgccgtattg ttagcgctgg cactgaacat ctgttacgtg
      421 ctgttgcgtc gcattaccgg cattcgcaca atcatgttga ccggccacat catgttccag
      481 caggccgggt tgattgccgt tacgctgttt atcttcggct actccatgtg gaccaccatt
      541 atctgtaccg cgattctggt ttcgctctac tggggcatca cttccaacat gatgtacaag
      601 ccgactcagg aagtgacgga tggctgtggt ttctccatcg gtcaccagca gcagtttgca
      661 tcatggattg cctataaagt cgcgccgttc ctcggcaaaa aagaggagag cgttgaagac
      721 ctcaaactgc cgggctggct gaacattttc cacgacaaca tcgtctccac ggcgattgtg
      781 atgaccatct tctttggcgc cattctgctc tccttcggta tcgacaccgt gcaggcgatg
      841 gcgggcaaag tgcactggac ggtctacatc ctgcaaaccg gtttctcctt cgcggtggcg
      901 atcttcatca tcactcaggg cgtgcgcatg tttgtggcgg aactctctga agcatttaac
      961 ggtatctccc agcgcctgat cccaggtgcg gttctggcga ttgactgtgc ggctatctat
     1021 agcttcgcgc cgaacgccgt ggtctggggc tttatgtggg gcaccatcgg tcagctgatt
     1081 gcagttggca tcctggtcgc ctgcggctct tccattctga ttattctagg ctttatcccg
     1141 atgttcttct ccaacgccac catcggcgtg ttcgctaacc acttcggcgg ctggcgtgca
     1201 gcgctgaaga tttgtctggt gatgggaatg atcgaaatct ttggttgcgt ctgggcggtg
     1261 aaactcaccg gtatgagtgc ctggatgggc atggcggact ggtcgattct ggcaccgccg
     1321 atgatgcaag gcttcttctc catcggtatc gcctttatgg ccgtcatcat tgtaattgca
     1381 ctggcttata tgttcttcgc tggccgcgcg ctgcgcgcag aagaagatgc agaaaaacaa
     1441 ctggcagaac agtctgctta ataaggagtt ttgattatga ccgtacgtat tctggctgtg
     1501 tgtggcaacg gacaaggcag ttccatgatc atgaagatga aagtggacca gtttttaacc
     1561 caatcaaaca ttgaccatac ggtaaacagc tgcgcggttg gcgagtacaa aagcgagttg
     1621 agtggcgcgg atatcatcat cgcttctacg cacattgcgg gcgaaatcac cgtgaccggc
     1681 aacaaatacg tggttggcgt gcgcaacatg ctctctcctg ccgactttgg cccgaaactg
     1741 ctggaagtga tcaaagagca tttcccgcag gacgtgaagt aaggacgcgc tatgaaatta
     1801 cgtgattcgc tggctgaaaa taaatccatc cgtctgcagg ctgaagcaga gacatggcag
     1861 gaagcggtga aaatcggcgt tgacctgctg gtggcggcag atgtggtaga gccgcgttac
     1921 taccaggcga ttctggatgg cgttgaacag ttcggtcctt atttcgttat cgctccgggc
     1981 ctggcaatgc cgcacgggcg tccggaagag ggcgttaaga aaaccggttt ctctctggta
     2041 acgctgaaaa agccgctgga gttcaaccac gatgacaacg atccggtgga tatcctcatc
     2101 accatggcgg cggtcgatgc caatactcac caggaagtgg gcatcatgca gatcgtcaac
     2161 ctgtttgaag atgaagagaa ttttgaccgt ttacgcgcct gccgtaccga gcaggaagta
     2221 ctggatctca ttgaccgcac caacgcggca gcttaagaag gaattgaaca tgtcattacc
     2281 gatgttgcaa gtcgcgctgg acaaccagac tatggatagc gcctacgaaa ccactcgcct
     2341 gattgccgaa gaagtcgaca ttatcgaagt gggcaccatt ctgtgcgtgg gcgaaggcgt
     2401 gcgtgcggtt cgtgacctga aagcgctcta cccgcacaaa atcgtactgg cagacgccaa
     2461 aattgccgat gcaggcaaaa tcctttcgcg tatgtgcttc gaagccaacg ctgactgggt
     2521 gacggtaatt tgctgtgcgg atatcaacac cgccaaaggc gcgctggacg tggcaaaaga
     2581 gtttaacggc gacgtgcaga tcgaactgac cggttactgg acctgggaac aggcgcaaca
     2641 gtggcgcgac gcaggtattc agcaggtggt ttatcaccgc agccgtgacg cgcaggccgc
     2701 aggcgtggcg tggggcgaag cggacatcac cgcgatcaaa cgtctttccg atatgggctt
     2761 caaagtcacc gtcactggtg gcctggcgct ggaagatctg ccgctgttca aaggcattcc
     2821 gattcacgtc tttatcgcgg gccgtagtat ccgtgatgcc gcttctccgg tggaagccgc
     2881 acgtcagttc aaacgttcca tcgccgaact gtggggctaa ggagcggata tgttgtccaa
     2941 acaaatcccg cttggcatct atgaaaaagc gctccccgcc ggggagtgct ggctggaacg
     3001 cctgcaactg gcaaaaacgt tgggcttcga ttttgtcgag atgtcggtag atgaaacgga
     3061 cgagcgcctg tcgcgcctcg actggagccg cgagcagcgt ctggcgctgg tgaatgcgat
     3121 tgttgaaacc ggcgtgcgcg tgccgtccat gtgcctttct gctcatcgtc gtttcccgct
     3181 gggcagtgaa gatgacgcgg tgcgggcgca ggggctggag attatgcgta aagctatcca
     3241 gttcgcccag gacgttggta ttcgcgtgat ccaactggcg ggctatgacg tttactatca
     3301 ggaagccaat aacgaaacgc gtcgtcgttt ccgtgacggc ctgaaagaga gcgttgagat
     3361 ggcgagccgc gcgcaggtga cgctggcggt ggagatcatg gattatccgt tgatgaactc
     3421 catcagcaag gcgctgggct acgcgcacta tctcaacaat ccgtggttcc agctctaccc
     3481 ggatatcggc aacctgtcgg cgtgggacaa cgatgtgcag atggagttgc aggccggaat
     3541 cgggcatatc gtcgcggtac atgtgaaaga caccaaaccg ggcgtcttca aaaacgtgcc
     3601 atttggcgaa ggtgtggtgg atttcgaacg ttgcttcgaa acgctcaaac agagtggcta
     3661 ttgcgggccg tacctgattg agatgtggag cgaaacggcg gaagacccgg cggcagaagt
     3721 ggcgaaagcg cgtgattggg tgaaagcgcg catggcgaaa gcgggcatgg tggaggcggc
     3781 ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca tggatctgcc gcgctatggg
     3841 ctggtgacct ttacctgggg caacgtcagc gctatcgacc gcgaacgcgg gctggtggtg
     3901 atcaagccca gcggcgtcgc ctacgaaacc atgaaagcag acgatatggt ggtggtcgat
     3961 atgagcggta aggtggtgga aggggaatat cgtccgtctt ccgacaccgc gacgcatctc
     4021 gaactctacc gtcgttatcc gtcgcttggc ggcattgtcc atacccactc cactcatgct
     4081 accgcatggg cgcaggcggg gctggcgatc ccggcgttag gcaccacgca cgctgactac
     4141 ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag aagaggtgca gggtgagtat
     4201 gaactgaaca ctggcaaagt gattatcgaa acgctgggca acgccgagcc gctgcatacg
     4261 ccgggaattg tggtgtatca gcacggaccg ttcgcctggg ggaaagatgc ccacgacgcg
     4321 gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg cgtggattgc gcgcagtatt
     4381 aatccacaac tcaatcacat cgacagcttc ctgatgaata aacacttcat gcgtaagcac
     4441 ggccccaacg cttattacgg gcagaagtag aacacgcgct gcggaaattt ccttcctcgg
     4501 gagataactg gtctaattcc gcagccgttt ttcaaaaaaa gccccctgcg aagggggcaa
     4561 agcaaactat ggcaatgttt cgttggttat acctggtgct agcgataaat atccgcgctg
     4621 gcgtgcatat tgccgttact ccccggttcc cgcatcagaa ttacgtggta gtacgttgcg
     4681 ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt cgctttcagt ggcgaaatta
     4741 tgattgatat aaatcacgcc taagctttgc acatcgtcca tatttctggc ctggtggtta
     4801 ttaatttcaa tggctgccca tgtatttgca cttagcaaaa gcacagccag aagggctaaa
     4861 actcgactga acatagatac ctcctcgacg gctgactttg tgtgctctcc tctgtgatga
     4921 tcttctgatt taattttaat caatgataaa gaagttgatg gtgaccattt ctgatgcagt
     4981 tgttcgaaaa aacaccatga tgaagtgtga tgaacttcaa atcagcgtgt tagtccactt
     5041 tggtgaaaga tgagatttat ttgtcggctg gccttgagtt tagcggggta tgtcagtact
     5101 ataacgcgtc atttttcagc cgacctttaa cacgttcctt gcctccccgg gattcggctg
     5161 acccagacag gaggctgaat aatccgtaag gagcaattcg atgcgtcatt acgaaatcgt
     5221 ttttatggtc catcctgacc agagcgaaca ggttccgggc atgatcgagc gctacactgc
     5281 tgccatcact ggtgcagaag gcaagatcca ccgtctggaa gactggggcc gccgtcagct
     5341 ggcttacccg atcaacaaac tgcacaaagc tcactacgtt ctgatgaacg ttgaagctcc
     5401 gcaggaagtg atcgatgagc tggaaactac cttccgcttc aacgatgccg ttatccgcag
     5461 catggttatg cgtaccaagc acgctgttac cgaagcatct ccgatggtta aagcgaaaga
     5521 cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc gctgatgatg ctgaagctgg
     5581 ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg ttgtccggca ccgtgtgcag
     5641 gactcccctt cgaaaggtca gtccatcagg aattcctcac tgccagttcg tgcttgagca
     5701 tcgttctgtg caggaggaag ccggctttca ccggcaggca tggtgtcaaa tgcccgttat
     5761 tgttagcgga cacgaaaacc aggccattac tcacagtata acggtcggca gtcgcataac
     5821 cgttcagggg ttcatttcat gccacaaggc aaagaacgga ctgagcaaaa tggttttgca
     5881 tgccgagcag attgaattga tagattctgg agactagcca tatggcacgt tatttccgtc
     5941 gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga gatcgactat aaagatatcg
     6001 ctacgctgaa aaactacatc accgaaagcg gtaagattgt cccaagccgt atcaccggta
     6061 cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa acgcgctcgc tacctgtccc
     6121 tgctgccgta cactgatcgc catcagtaat cggtcacggt ccattaatac gactttgaga
     6181 ggataaggta atgcaagtta ttctgcttga taaagtagca aacctgggta gcctgggtga
     6241 tcaggtaaac gttaaagcgg gctatgctcg taacttcctg gtaccacagg gtaaagctgt
     6301 tccagctacc aagaaaaaca ttgaattctt cgaagcacgt cgcgctgaac tggaagctaa
     6361 actggctgaa gttctggcag ctgctaatgc tcgcgctgag aaaatcaatg cactggaaac
     6421 tgttaccatc gcgtctaaag ctggcgacga aggtaaactg ttcggttcca tcggtactcg
     6481 cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg gctaagagcg aagttcgtct
     6541 gccgaacggc gttctgcgta ccactggcga acacgaagtg agcttccagg ttcacagcga
     6601 agtattcgcg aaagtgatcg taaacgtagt agctgaataa ttcgttattc aacgagacgt
     6661 aaaaacgccg accactggtc ggcgttttgc tttctatttt tcgtcaggta ttagtttcgc
     6721 aagtagatcc attcaccatt ctcttgtgtc cataagtcag gatggtttgc tggcgttaga
     6781 tacatacgag cgcaactgat gtctcgctcc tgcggcactc gctcgaaacc caaacccgag
     6841 tagattgcgg ctgaacttga taaggaacgc agaccaattt tcaaatcatg gggtaaatca
     6901 gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg cattagaaac gcctgtaaca
     6961 gtgcgaagtt ggtcgatata aagatcgatt gttggtagtt cagatgacca gcaattaggc
     7021 atttgggcag gaagggttac aacgtatcct atcgtttggc cggtatagtc tggagtacca
     7081 taaccaggtc ccaaaaccac ttcgtgtatt gtgtatttac tgttgggttc gaaggattcg
     7141 acatcttgca ataaatattt cagttctgcg gttaacctcg cacccgcgta agctttattg
     7201 tatttctggg cgtgaatatt atctaatgta gcttcatggt tccatccaaa agaattatca
     7261 ccattcattg ttactccatc gtaagcatta cacttcgttg ctaacttatt gaatgcttgc
     7321 cagtctgaga gatctagttt gtggatctca acagtacgtc tgccatcgca attatgatct
     7381 cttccacgat ttgaaagtaa tgtagcgcgt gcgtattgat gaaattttaa ttttactggt
     7441 ttgtcggtgg acgaaaaacg agataaatat gcaggaa
//
LOCUS       DBFIFS010000214         7473 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.214, whole genome shotgun sequence.
ACCESSION   DBFIFS010000214 DBFIFS010000000
VERSION     DBFIFS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.214"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1726
                     /gene="adeD"
                     /locus_tag="N7A07_003997"
     CDS             <1..1726
                     /gene="adeD"
                     /locus_tag="N7A07_003997"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU2478564.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQPVSASDFALQFTPGK
                     RYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFA
                     FTTLEVTE"
     gene            complement(1772..3163)
                     /gene="uhpT"
                     /locus_tag="N7A07_003998"
     CDS             complement(1772..3163)
                     /gene="uhpT"
                     /locus_tag="N7A07_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU2478565.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3301..4620)
                     /gene="uhpC"
                     /locus_tag="N7A07_003999"
     CDS             complement(3301..4620)
                     /gene="uhpC"
                     /locus_tag="N7A07_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU2478566.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4630..6132)
                     /gene="uhpB"
                     /locus_tag="N7A07_004000"
     CDS             complement(4630..6132)
                     /gene="uhpB"
                     /locus_tag="N7A07_004000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU2478567.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6132..6722)
                     /gene="uhpA"
                     /locus_tag="N7A07_004001"
     CDS             complement(6132..6722)
                     /gene="uhpA"
                     /locus_tag="N7A07_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU2478568.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6884..>7473)
                     /locus_tag="N7A07_004002"
     CDS             complement(6884..>7473)
                     /locus_tag="N7A07_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478569.1"
                     /translation="SFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDGIKPEKI
                     AKIISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGR
                     NYHQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVH
                     SDESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
BASE COUNT         1917 a         2100 c         1907 g         1549 t
ORIGIN      
        1 ggcggaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
       61 tgataatgtc tctattctcg acctgattaa tggcggagaa atttccggcc caattgtgat
      121 taaagggcgt tacattgccg gtgttggcgc agaatacact gatgctccgg ctttgcagcg
      181 gattgatgcc cacggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
      241 atccagcatg atgacgccgg tcacctttga aaccgccacc ctgcctcgcg gcctgacaac
      301 tgttatttgc gatcctcatg aaatcgtcaa cgtgatgggc gaagccggtt tcgcctggtt
      361 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc
      421 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
      481 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattag
      541 cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc tggacggtca
      601 ctgcccgggt ttgggtggta aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
      661 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
      721 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
      781 taacagcccg caatgcatgc tctgtactga tgaccgtaac ccgtgggaga tcgcccatga
      841 aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
      901 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
      961 actggcacct ggtaagcagg ctgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     1021 gcagcaggta ctggtgaaag gcgaaccgat tgacgcgcaa accttacagg cggaagagtc
     1081 ggcgaaactg gcacaatccg ctccgccata tggcaacacc attgcccgcc agccagtttc
     1141 cgccagcgac tttgccctgc aatttacgcc cggaaaacgc tatcgggtca ttgacgtcat
     1201 ccataacgaa ttgattacgc actcccactc cagcgtctac agcgaaaatg gttttgagcg
     1261 caatgatgtg tgctttattg ccgtacttga gcgttacggg caacgtctgg ctccggcttg
     1321 tggtttgctc ggcggctttg gtctgaatga aggcgcgctg gctgcgacgg tcagccatga
     1381 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctag cggtcaatca
     1441 ggtgattcag gatggcggcg ggttgtgcgt ggtacgtaac ggccaggtcc aaagccatct
     1501 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggagcaaat
     1561 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     1621 gatggcgttt ctttccctgc cagtgatccc cgcgctgaaa ctaaccagcc aggggctgtt
     1681 tgatggcgag aagtttgcct tcaccacgct ggaagtcact gaataataaa aaaagcccgg
     1741 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     1801 tctcgcggcg gattttgcgt tcttccatca ccgccactat cgccatcagg cagatacaac
     1861 caatcgcggc gatatccagc gcagcgaagg tgcctgccca gccggtaagg ccgaatactg
     1921 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     1981 cgaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     2041 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     2101 ccagatagat atattcgttg ctggcatgtt ggtacacacc gagcgtggcg ataatcagcg
     2161 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc taggtcagag agccagcccc
     2221 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     2281 ctttagagag tttcagttcc tggaacgcgt acacagttga ccactggtcg ataccaatac
     2341 gcaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     2401 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     2461 cgctgatctc ctcgccgaac agctcttcag ctttgccgag gccataagat tccggggagt
     2521 cgctgccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     2581 acatgccgat gacatggcca tcgaacaggt aatttgcgcc gaacagtgcc acaccagctg
     2641 cgcctgcacc gccaaggttg tgagaaatgt tccagaaacc gaggaacgtc ccgcgtttac
     2701 gacgcggcgt ccatttggtg atggtggagt aactgcacga acccccggta ctctggaaaa
     2761 agccacttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     2821 tggcgctaaa gcccagcata caaatggcag acaggatcag catgaacggc aggaattgtt
     2881 tggtgttttt gccgtcggcg tagtaggaaa ccagcgtttt acccacgcca taagtgatgg
     2941 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     3001 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     3061 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     3121 ggtccagggt cggcttgcgg acctggttta agaaagccag catgggttac tcctgaaatg
     3181 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     3241 caggcaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     3301 tcacgcttcg cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     3361 ggcggcaatg gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     3421 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     3481 cgcccctgcc gcctctttgt gggaacattc tgccgccgcc atgccgatta acatctgcgg
     3541 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     3601 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcagcgaaaa tcaaattcat
     3661 cggcccgcgg ttgccgttaa acagtttgtc cgagccccaa cccgctacca gcgcaccgat
     3721 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
     3781 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccgaacca catagaccag
     3841 cacatagcaa aacgaaagca gccagatata cggattcaac aacacatact tggtgaggat
     3901 ctctttacgc gtcagccctg ccccttcttg ttgttgagca atctccagcg cgtcgtgtcg
     3961 ccattcaccg accgctggta aacctaacgc ctgcgggcga tcgcgcagcc gccagcagag
     4021 aaaaatcccc acgacgatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
     4081 atgcagcgca gctgctgcca tcacaatggg aatgagcgca ccgccaacgt tatgcgccgt
     4141 gttccataat gcccaccaac caccgcgttc ggtacgagaa taccaggccg ttaacagacg
     4201 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
     4261 cgcccatagc gacgtcgaga agccaaacag aatgttgatg atgcccgtgg cgataagccc
     4321 tatccccata aaataacggg catttgagcg gtcgctgaca atgccggaga caaacttcga
     4381 cacgccgtag gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
     4441 gccattagca aggatttctg gtacggcggc gttaaaactt ttacgcgtga agtaaaacag
     4501 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
     4561 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
     4621 catcaaacct tagacatagc gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
     4681 ggaaatggtc aaggtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
     4741 accttgttgc ccggaacccg gcggtaaacc gctgccatcg tcttcaatca ccagcatcaa
     4801 ccgttcatcc tgcagccagc cttgcagggt gaccgcgctg gcatcagcat gtttcacaat
     4861 gttattcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaacgc
     4921 tgactcttcg attcgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
     4981 ccgcatcagt gagcggatgg cctgctctag ggtgagatca tccaactggc gcggacgtaa
     5041 ccgccctaac aaacggcgca ccgcgtcgta cacgcccagc gacagttgtt cgatgagctg
     5101 cccgctctgc ttcacactgg cgttatctgc cgccagtcgc tggacaatgc ccgcttgagt
     5161 acgaatggca gtgatggtct ggccgatatc atcatgcagc tcacgcgcca catcacggcg
     5221 cacgctctct tcagtttcta acaaacgttc agcgagatgc tgattgcgcg ccagttcctt
     5281 ttgcagcgac tggttaagtt cacgcaaccg ctggatgcca gcgcccagta acaaccctgt
     5341 cagactttgc accagcagcg agagcaataa atccacagga tgatcgcgcc aggtttgact
     5401 ggcgatcagc gcgatggcgt tcatcaatgt cgctatcagc gccccttgcc agccgtagtg
     5461 ccaggccagc gcgataatcg gcagcgccag gcagaatggc gtaaagcgcg acagttcgtc
     5521 cggcaagccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
     5581 ccgcccgcgc cagttgatcg gttgagaaac taacgacggc ccgagcggca gccaggtgtt
     5641 attggcgaga taatgccaga acaccagaca tatcggggcc agcgtcaggc cgccagttaa
     5701 agttaacagc agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
     5761 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgttgatg
     5821 gcgatagcgc gagatcagcg ccacgggcag taacgtcagt aaactaccga tcatcaataa
     5881 ggagaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgctcc
     5941 cagcagcacc ggccagtatc cgcgcgggca ttgcagcatt agccctagcc gcagaccaaa
     6001 cggaaataac agcaccgcca tatcagggcg ctcaaccagg tgcaggctga tactccacag
     6061 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
     6121 caacgtcttc atcaccagcc atcaaacata cggcgcgcca gttcaacgtc gttactgacg
     6181 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
     6241 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctgccgttca
     6301 cgtttggtta gtgggtcctg gcgaccggat gccagtttaa tggcaatatc cggcgtcaga
     6361 taacagccgc ctgtggcaac cgtatgcacc gcagcaatca gttcgtcagg gctacaacgc
     6421 ttggagagaa agccacgcgc ccccgcgtta agcgcctgct caaccagcgc cggactgtca
     6481 tgaacggaga gcataattgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
     6541 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
     6601 gccagcgcct cgcgccccga accaaactcg gcaactactt gcaaatcagg ttccagcccc
     6661 agcaactgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
     6721 atgattgggt cctggcggat aaaaaacgcg cataccttaa cgataagcgc gatgttgttc
     6781 aagttggata tggggtatgt attggcttaa tgatgttgag cttatcgtca aaacgcttac
     6841 ctgagaggta agcgtttggt gcgtgttatt gagaaaaagc gaattatttg taggttgcaa
     6901 tcgtaatatt gcctgagaaa tttctcctta gattgcggat atcattatcc cttgcccaat
     6961 aatcatcttt gtgtctataa ctctcatcgg aatgtacata gggagaatca tcttcagcgt
     7021 cgttatggat aatattttcg cctttagtgg tatagatata attagcaaca tcaaaggtgt
     7081 cattcgcgtt atttacgact gcgcctaaga cgcaaagggt ttgagtgaat ccgtattctg
     7141 agaaatcgac atgctgatgg taatttctac cattcaaagc aacaaaactc ggacaaacat
     7201 ctattgtatc gccaacagac gtagcggcgc gacgcacatc aagtgcagcg ttatcgttag
     7261 caacatgaaa tgcttgtgaa caggcataaa acaaatttct aatcccggta cgactggata
     7321 aacaagaaat aatttttgct attttttctg gctttatacc atcctgtact aaagaatctc
     7381 tcaccagatc acattcacca ggtgtgaaaa caactttctt gaatatttcc tcataaacat
     7441 cataagaaaa atattcagag agagtgaaag att
//
LOCUS       DBFIFS010000215         7429 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.215, whole genome shotgun sequence.
ACCESSION   DBFIFS010000215 DBFIFS010000000
VERSION     DBFIFS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.215"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..128)
                     /locus_tag="N7A07_004003"
     CDS             complement(<1..128)
                     /locus_tag="N7A07_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2478570.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGAR"
     gene            complement(228..1079)
                     /locus_tag="N7A07_004004"
     CDS             complement(228..1079)
                     /locus_tag="N7A07_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HGU2478571.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1506..6092)
                     /gene="ypjA"
                     /locus_tag="N7A07_004005"
     CDS             complement(1506..6092)
                     /gene="ypjA"
                     /locus_tag="N7A07_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HGU2478572.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6157..6276)
                     /locus_tag="N7A07_004006"
     CDS             complement(6157..6276)
                     /locus_tag="N7A07_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU2478573.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6500..6658)
                     /locus_tag="N7A07_004007"
                     /pseudo
     CDS             complement(6500..6658)
                     /locus_tag="N7A07_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7028..>7429)
                     /locus_tag="N7A07_004008"
     CDS             complement(7028..>7429)
                     /locus_tag="N7A07_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478574.1"
                     /translation="DDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQ
                     YVNWGMSLFDVDTPCIFNTPANHESYEKSLKPVSENGLNGVLSDRNKKIKMITGVAPF
                     DDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
BASE COUNT         1840 a         1858 c         1594 g         2137 t
ORIGIN      
        1 cgcgcaccga ttttaacggg ggaaggaaaa cgattttggt tgatgaggtg atagatccaa
       61 gctttaccgt atccagttcg atgcagaacc tctggtaaac gaataaggcg ggtgttgtgc
      121 tgattcatag tcgtttcctc tgtgccgttt tgatggaaat gactatacaa atgaatcagc
      181 aggaaaaaga ccgaagctgg gaacgccaga gctaatggag gttggtctta gtcagaatca
      241 gcgattttca tcacgtctga aactctgatc tttttcaaat ggctgttctt gttgatgaac
      301 agattaaact ggcgaataaa aggtatactc gccgccttca aagcaagagg tttgtctgta
      361 tccctgattt ggtggttcag gcgagtgttg atctcatcat cgtcatctgt atacaacagg
      421 cgagaaaggc ggtcttctga tatacgtact ttatataatt ttgaccatac cagaatgtcg
      481 atcatgggaa ggagtctgta attaatgatt ttcttaatcg ttccataacc aaagcgaata
      541 gcttccgtta catctggttc tatgccacgc actttacgcc attgaggaag tgcagccttt
      601 agggattcag cgatctcttc atctgtcccg tttgctaggt cgatttcgac catgacagta
      661 tccctgaaca attttggcaa aacttccgat acagggctat cgaggaattc tctgttaaca
      721 gaatactcat cgcttccatc ccaaaaaaac agtgagttcc gtaatccaac tatgctgagt
      781 tgagccagtc tttcggttgt cgtcaaaaag aagtgcgggg gctggtaaag agtatcctct
      841 cttgtgagat agccgagatg cttttctgtg atgagaaagg gctttccagt gaagatcctg
      901 tcgatgtaga gccttacggc ttctgcttca aattcttcat gatatggttt gaagaacaat
      961 gtccttgcca gcaattcatg gtagaggtgt ataagcggaa gctcttcgaa tccgcgatat
     1021 gtatctatat ccagccactt cttcacctct ctcgtatgtt caactgacca gtttttcata
     1081 acgatctcca acaggcatta gacttaatgt atgtagcaaa cgtaatcgac atgttaaagg
     1141 aagaccaatg aaatgagttc agatgccatc aggaagttga accaaaaggt ttgatatttt
     1201 ttgatacagt tcgctaacgg actattttca ggggtttatg ccgttacgat ttatggttcc
     1261 tttatagaac caaaggtaaa gtcagaaaca gcagttatct agaaaattca atcaaataga
     1321 tatctagtta cagggacgcc agccaatcat gattggacgg tgtaaggaca acaccaacaa
     1381 aaacaggaag ttagaagtct cagcaggaca ccgaccagac ggtgaggaga cataaaagga
     1441 tacgcaaagg agccgcggct cctggtgaca tgaaagccca cagatgtggg ctttttcgtt
     1501 gatggtcaga acgaccagtt cacaccagct accgcgttcc acggggattc cacaccggca
     1561 ccatggctat accccacccc aagatgcccg cttaacgtac tgctgaatga ggctttaata
     1621 cctgcctggt atattccacg tctgcccgac aaatcattga cgaaattacc gtcattattc
     1681 accttcaccc ggttatcatc gacaaattct ttgcgcacag ccgccttcag ccacggctca
     1741 atttccatac cgttccccag acgcatgttg taactcagcg ttgcgcccag ttcacgatat
     1801 atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata ttcggggtta
     1861 tcagcggtga accccgttaa cgatgcatac ggcgtcaggt tccagttacc atcggtaaat
     1921 cgcatcccgg tttcaatgtg accgcccagc ccgttgctgt ggtaactgcc attggcggct
     1981 ccaccgctgc tcattttacc ggctacgtta ctttcaaaac ggttcagctt cacgacaccg
     2041 tccagataga aaccactttc atgttcccaa ctggcatagc cgcccagaga ataactgccc
     2101 acactgccat gtcctccgcg atcaaaaccg atatgtgaat gggaataacc cataaaagcg
     2161 cccagcgtcg caatcccctc aggaatatca ttagggctgt cgatccccac tgtcattccg
     2221 gtcagcgtct gctcaaaccc ggcccccgca tcggtggtga cattattacg ggtgttatac
     2281 gtcgcccccc agacattatt gttgtgtgga ctcgctttca ttatgttcaa ccgctcgcga
     2341 atactgttta gctcagcatc aaataccaac ggtaatgttg ctgccatatt gagtacggct
     2401 gcggtagaag gcgtgatgcg tttctccgga acgggtgtcg gcgttggctc gggagtcggg
     2461 tctggtttcg gatccggttt tgggtctggt tttggatccg gcttcggatt tgggttggga
     2521 tttgggttgg gatccgggtt gggtttgaca tcattggtca ggttccagtt gctgttgcca
     2581 tcgcttttca ggacatactc ataggtccca agatcaacga aaccgccagt attgcccagc
     2641 gaaaacgaag catcccctcc ccctgttttc accagcgtca tcgcgtcgtc agactgagga
     2701 ctgacgccgg tatcctgaac aaagatttta aaattaccag tggcgttgtt gttgacgacc
     2761 agttgatcgc cccgagagcc tgcaacgttg gtatgcaggt agaaattacc gctaccggaa
     2821 agttcattgg ttgtcagcgt attgtatata ccgttagttg tggcaggcgc tgctgtttgt
     2881 gctgataaat aaacattacc gtcgttaagt agcaaactgt ttactctata ctcgcagttg
     2941 ctggtgcctg cacaggaatt attgctgtta agccagacac tgccccggct ggcagccgtc
     3001 aggtcggcaa gcgtcgtatc aacgccattg ccgatagtta atgtcgcgct atcggtaatc
     3061 cggatcgccc cttcgagttt aactggcgta acattatccc gtggcgtcat taacgacagg
     3121 cttcctgtcg cgccactgac cgatgcatcc gccagcgtac ctgcgtagac gattgctgtc
     3181 ccgccagcaa cctggagatc ttctgcccgg gccagagcct gaaactcatc ttttgaccgc
     3241 ccaagggtat attgcccacc agagttaacc tttgtggcgg agtcctgacc ctggttttgc
     3301 attgcccccc ccttgccaac cgtggagtcg cgagcttcgg tacctgctaa taccaataaa
     3361 ttaccgccat tttccagcaa catattggtc gctaaattgc cggaaatgga aaaagtgccg
     3421 tactggtgag taccgctgat ttcaataccg ttagccgtgc tcgtctggag agcggcaccg
     3481 ctgttctgga cgatatctgt cgctttgcca ttatcgttga ctgtcagcgt accgccttca
     3541 ttgatcgttg tttttattgc ctctccgtta gctgaaactg tttgtattcc gccgtcgtta
     3601 attgttgtct cattcgccac accctcgaca atttgttcac cgccggtgag cgtcgtgcct
     3661 gtcgcagtgg cttttgtttt gacgatctcc cgtccgcccg tattgacctg tgttttgtca
     3721 gaagaggtgt ctgactccac ggttaacact ccgccatttg ccagcaggat attgttcgcc
     3781 gcaccctgct cgatgctgaa cgcgacgcca tccgcgcgtg ttcctgtgac ccgcgtcgcc
     3841 ctggtggttg caaccaacgc gccctggcta ctctgctgta tccccgttgc gctgcctttc
     3901 tcccgcacat cgagtgtgcc gccatcatta agcaccgagt tttcagccag accgccctca
     3961 ttaactatct gtgaaccgcc attaataatg gaaccttccg ctgtcccgtt tgccataatt
     4021 tgttgtaggc cagagacgat atcggtattg atcgccttac cataattctg aacggtttgc
     4081 gtgccaccat tgatgtgtgt tttctctgtt gacccaccat caacaatttg ttcaccactt
     4141 tcgatatttg cttcagtggc taaaccatat accgtttgct tgccaccttt gatatttgct
     4201 ttatcagaag tggcactggc atatattgtt tgggtgccag cactattgag tacagtgcca
     4261 acatcttttc cataaacatc cattttgccg ttggcattaa taatcgtgtc aactgcccgg
     4321 gaaccagtga cgactgttaa tgagccagcg ttttccagca ctacattttt agcttctgaa
     4381 ttcctgatgt agaaagcatc accataactg ttggttcctt cgataagtgt tccggaggtc
     4441 gtggaagcaa ttaatgcgcc gccggattgt tgctcaacat gcttagcctc accaccgtcc
     4501 tgaacctcca gaacgccgcc attattaagt ctggtgctat ctgttttagc ctccttctgg
     4561 acaatcagct taccgccagc atcaatggta gtatttttcg ccgaggtttt agccactacc
     4621 gtcagttcgc cggtattttc cagcacaaca taattagcct cccctccggt aatagtgaag
     4681 tgagagagct tgttgtatcc ttcgatatca gtccctgcac ccgtgttggc aactaaagca
     4741 ctgcccgtct cctggttaac cccatgtgca ataccgccgg tatagacaat cagcgagcct
     4801 ccggcgctaa tattgctgcc aattgccgta ccatctttct caacaacctg ccggctcccg
     4861 gaggatataa ttgttgtgtc agctttcccg ccgcttttga tattttgcgt tccgctgttg
     4921 atattggtgc ctgtggctat gccataatta ttaatattct gtgtgccacc attaattatg
     4981 gtatttgtcg cgtttcctgc aacatccata accccgccat tatctattcg ggtcgcatca
     5041 gctttagcat tggttaaaac tgacattgtt cctttatctt taataatcgt cttgtttgcc
     5101 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
     5161 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
     5221 ttagttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
     5281 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgtt atcaataatt
     5341 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttactatt cagagactga
     5401 gttccgcctt caatcgtcgt gtccagcgca cggctctcat atactctttg ctcaccgcca
     5461 tttttgatgg tggttgtttc tactgtgctc tgttcaacat actgtcgacc accatttatg
     5521 gttgtgttcg ttgccagact tccttgtact acgtcctgag agccagactt atttatcgtt
     5581 gtaccatcag catgcccttg aacttttact atctggctac caccatcaat gagtgttcca
     5641 ttcgcactcc ctccctctac gcgtgaagca ccgcccttaa ttgtcgttcc attgctgata
     5701 ccccctttat aaacgtcctg attgccactc tcgattatcg tacctgtgga aatacccccg
     5761 tcatgaattg actgtctgcc cccgttaatg gtggtattat tagactgccc cacataattg
     5821 ttatgccttc ctacatcttg atatccacca gattcaataa gacttccatt agataccccg
     5881 ccataaacat tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     5941 tctactcgtt ggctgccatc tacagtctca tcgtttacca caccaataac atcaggagtg
     6001 aaggcagcca tcccgggcgg ggcatatatc aaggcagata tcaataagga aagtactgag
     6061 cgacgacaat aatagggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     6121 gagtagcgtt taagcgacct tagctttgct gcaacatcag cccacaggca ccagaccagg
     6181 ggattcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     6241 gatgaaatga tgatattccg cataagaatg aggcattttt taaacgcagt gcgctgaagt
     6301 gtggttggat aaaaaagtca atccattcag gaaatacggg cgtattcttt tctttcgaca
     6361 atgaggccgt tggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     6421 cacataaaaa tcacacaata actttgagat cgcagattgt tttactttta cagcattcgt
     6481 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgttcgc
     6541 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg ttcagtttac
     6601 agactagcag tgctttccct gccgatgatg tcctgagcag ttttttcagt ccggacctcc
     6661 tgccacggtt ttacgttgaa gcggtaaccc tgagcacgca gttcttcagt caggcgtggt
     6721 gcaccgtaac gctgttattg ctgggtgaga tcaaaaaact ttcaggcagc taaggaaagt
     6781 tgaaccagac aatagatgaa atatttcaac caattacagc accaattcag tcactgccag
     6841 cccaccaaat aaatcaaggg gttacatgaa aacgtagccc ctttttcttt ggtagtgaca
     6901 ctaaaatgga tgtagtgtga agaataatcc cgtttactca atcaataata catattgttt
     6961 caatctacgt tattatctct ttataaaaat tgccatttat ttatcattga aaactgcttt
     7021 tagaacttta tacaagagga ctattcacaa caggactatt ctcaatggga gcatcctcag
     7081 gggagttatc atcaaagtca tcatccataa ataaaatatc atcgaatggt gccacacccg
     7141 tgatcatttt tattttttta ttacgatcag ataagactcc atttaaaccg ttttcgctta
     7201 caggttttaa tgatttttca taactctcat ggttagcagg cgtattaaaa atacacggag
     7261 tatcaacatc aaacaatgac attccccagt tcacatattg aatatcatag ttactgaagt
     7321 tctgtccaga aaagaagcat cccttaaaat ccaatccacg tagattatat aagccaccct
     7381 cttctttttg gagagtaatg ttaattttgg ctatctcccg gacatcatc
//
LOCUS       DBFIFS010000216         7422 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.216, whole genome shotgun sequence.
ACCESSION   DBFIFS010000216 DBFIFS010000000
VERSION     DBFIFS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.216"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(180..815)
                     /gene="nth"
                     /locus_tag="N7A07_004009"
     CDS             complement(180..815)
                     /gene="nth"
                     /locus_tag="N7A07_004009"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU2478575.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(815..1510)
                     /gene="rsxE"
                     /locus_tag="N7A07_004010"
     CDS             complement(815..1510)
                     /gene="rsxE"
                     /locus_tag="N7A07_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU2478576.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(1514..2134)
                     /gene="rsxG"
                     /locus_tag="N7A07_004011"
     CDS             complement(1514..2134)
                     /gene="rsxG"
                     /locus_tag="N7A07_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU2478577.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(2138..3196)
                     /gene="rsxD"
                     /locus_tag="N7A07_004012"
     CDS             complement(2138..3196)
                     /gene="rsxD"
                     /locus_tag="N7A07_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU2478578.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3197..5515)
                     /gene="rsxC"
                     /locus_tag="N7A07_004013"
     CDS             complement(3197..5515)
                     /gene="rsxC"
                     /locus_tag="N7A07_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU2478579.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(5508..6086)
                     /gene="rsxB"
                     /locus_tag="N7A07_004014"
     CDS             complement(5508..6086)
                     /gene="rsxB"
                     /locus_tag="N7A07_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU2478580.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(6086..6667)
                     /gene="rsxA"
                     /locus_tag="N7A07_004015"
     CDS             complement(6086..6667)
                     /gene="rsxA"
                     /locus_tag="N7A07_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU2478581.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(6744..7184)
                     /gene="ydgK"
                     /locus_tag="N7A07_004016"
     CDS             complement(6744..7184)
                     /gene="ydgK"
                     /locus_tag="N7A07_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU2478582.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(7270..>7422)
                     /gene="ydgT"
                     /locus_tag="N7A07_004017"
     CDS             complement(7270..>7422)
                     /gene="ydgT"
                     /locus_tag="N7A07_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU2478583.1"
                     /translation="LYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQVPKS
                     VWHYVQ"
BASE COUNT         1817 a         1978 c         1912 g         1715 t
ORIGIN      
        1 tgatcactaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc atttttggtt
       61 atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac gttaaaaaga
      121 tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac cccttttctt
      181 cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga gccacagcgg
      241 ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg gcagtcgact
      301 ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac gtttttcccc
      361 ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc
      421 cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc gggcagggct
      481 tcaagcgcag cacgatcttc cggaacctcg ctattatgct gctccagtaa gatacggcag
      541 gttttgatga tattttctgc tttgctgtta taaagcccaa tcgttttgat ataggttttc
      601 accccttcaa cgcccagttc aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc
      661 gccgtcgcct tattaacact gacatcggtt gcctgagcgg aaagcagtac ggcaatcagc
      721 aattcaaaag gcgaactgaa attaagctca gtggtgggat gaggattgtt ctcacgcagg
      781 cgagtgagga tctccaggcg ttttgctttg ttcatcagac attccctgtt tcaccgtttg
      841 gcaatgcacg ttcggcagct gcttcagtac ggcgcttttt cattctttca tcaatcaggt
      901 attttcctgc cagcatcagt cccaggccaa taaatgcacc tggtggcagc atcgccagca
      961 ggaaagggga gtcggtgtgg aaaatctcca cgcgtaatac ttttgcccag ctacctaaca
     1021 gcgcatctgc accgtcaaac aatgtgccat tgccgataat ttcgcgtagt gaacccagca
     1081 cgaacatggc gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg
     1141 gacctttttt ggcagcgaag gcttcagcgc ggcccacaac gatacagtta gtgacaatca
     1201 gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg atcagcatct
     1261 gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg atctcggctg
     1321 gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg
     1381 caagtcctaa acccagagcg ttagtggcag tagacgtgac agccaacaga ggacaaaggc
     1441 cgagcaactg gaccagcgca gagttgtttt tccacaaccc ctgaacaata acgtctttaa
     1501 tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgttcc ggtaacgtct
     1561 gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta atcgtcgcgc
     1621 cggtgaactg gtcgaaatta ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac
     1681 cactgatttt tttacccgca aaatgggtga tccagtcaga aaggcgcagt tcgattttat
     1741 cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc gtgccgttaa
     1801 aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg gttgcttcca
     1861 gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc tcacctttac
     1921 ctaactctgg cgcagttacc agatagcaac tctgtgccag cgcattgtta tagcgttcgg
     1981 ctggcagcac ctgatcaaat aaagcctttt gttgcagact ggcctgttca gcaatcgtcg
     2041 ttttggtcat ctggttgatg gccgcagtta accctgttga acccgctgca aatagcgcca
     2101 acgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga tggccgtaga
     2161 cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc aggacggcaa
     2221 aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat aagcccgcaa
     2281 gcgcgccgaa aataagacga ccacgattgg tcgtagaagc ggtaaccggg tcagtcaaaa
     2341 taaagaatgc gccgagcatg gtcgctccag acagcagatg aatttgcggt gctgccagtg
     2401 tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact aagaagctga
     2461 ggggaatatg ccagcgaatc gctttctgcc atagcaacca tacgccgcca gccagccagg
     2521 cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc ggatattgca
     2581 taatctgttc aaccgaatga ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg
     2641 cctgactaat gccatcaata cctaagcgta gtgtgttcat atcaccacca ctggcggtat
     2701 gcccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc gcaatttcat
     2761 gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc acataaccaa
     2821 tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta gcgatgataa
     2881 ccgcaaacac cgtacccagc acgaccatcc accatggcgc gaggggggga atacttaccg
     2941 ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgtggcggct accgactgct
     3001 tgcgtagttt gagtacgaga gcttcggcta acagagcact aaccgatgcc aacaggatct
     3061 gaacgagagt accccaacca aaaaaccaca gttgcgctgc gattcctggc acggctgcga
     3121 gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa ggggagctag
     3181 ctattctgaa taccatttag tcctcgttta caaccttttg ctggacggct tttttggcct
     3241 gaacgcgggc aatagccgcg gcaactgccg ctttgcgcgg atcaacctgt tcttctggtt
     3301 ctgcattagc ctgttgctgt tccagcttgc gtgctttggc gcgtgcaata gccgctgcga
     3361 cggcagcttt gcgcggatca acctgttctt ctggctccgc attagcctgt tgctgttcca
     3421 gtttgcgcgc tttggcacgg gtaatagcgg cttcgacggc ggctttgcgc ggatcgaccg
     3481 gttcttctgg ttccgcatta gcctgttgct gttccagctt gcgtgctttg gcacgggcaa
     3541 tagcggcttc gacggcggct ttgcgcggat cgaccggttc ttctggttcc gcattagcct
     3601 gttgctgttc cagcttgcgc gctttggcgc gggcgatagc ggcttcgacg gcggctttgc
     3661 gcggatcgac ctgttgttct ggttccgcat tagcctgttg ctgttccagc ttgcgtgctt
     3721 tggcacgggc aatagcggct tcgacggcag ctttgcgcgg atcgacctgt tgttctggtt
     3781 ccgcatttgc ctgttgctgt tccagcttgc gcgctttggc gcgggcgata gctgcttcaa
     3841 cggcagtttt acgtggatca gtaacggttg ctgcgtcgtt agtttgctgc agttctgcct
     3901 gtttcgctct ggcttgcgct ttacgggctt cccgtgctgc aataattgca ctgttatccg
     3961 ggcgttcgcc cgctttaatc acaataggct gtgtagcctg ggcctgtttc tctttcaccc
     4021 gcgccagagc ggcagcaatc gcatctttat ctttggctgc aggttgaacg gctgcgctct
     4081 tatgtcgttc aaggcgagcc gctttttcgc gctccagacg agcctggcgc gcttcgaaac
     4141 gcgctttggc ttctgcggcg cgcttttctt cctgacgaat agccgcaatt tcagcttttt
     4201 cctgacggaa atattgcacc aggggaatat tgctcgggca aacccacgcg caagccccac
     4261 attcaatgca gtcggcaatg ttatgcgtgg tagctttatc gtgttgctga cctttgctga
     4321 accagtacaa ctgttgcggc aaaagatccg cagggcaggc gtcagcacag gcgctacacc
     4381 ggatgcagct ttgttcttcc tgtggttcgc caagctcatt ggcagaggga gccaacagac
     4441 agttggtaat ctttacgacc gggacatcca gccatggcaa ggtaaagccc attagcgggc
     4501 cgcccataat caccatttga tcggcagagg ggcagaatcc ggcgtcattc aataaatgac
     4561 gcactggcgt ccccagccgt gcccagacgt tgcccgggcg agcgattgct tcgccagtca
     4621 gggttacgac acgctcggta atcggctcgc catcaataac ggcacgtttc actgcataag
     4681 cagtgccgac gttttgcatt aatacgccga tatcggatga acgcccgcca tgtggaacct
     4741 gcttcccggt cagaatgtag gttaattgtt tagcaccgcc agaaggatat ttggttggaa
     4801 tcacccgcat agaaatatcg tgagagtccg ccagcaccgc gcgcagcatg gaaatcgcct
     4861 gcggtttgtt atcttcaatg ccgataagaa tttcgcgtgg ctgcagaata tgcgcaagaa
     4921 tgcgaatacc ctctacgacc tgagccgcgc aatcctgcat caaacggtca tcggcggtaa
     4981 tgtatggctc gcactcagca gcgttgataa tcaacgtctc aatcttatct ccgccgccct
     5041 gcaatttaac gcctgtcggg aatcctgcac cacccagccc ggcaacacca aactgatgaa
     5101 tgcgctcgat taactcttcg cgacggcgag agcgataatc ggcccagccg tctcgtggga
     5161 tccagcagtc ttcaccatcg gcatcaataa tcacgcttaa ttcagctaaa gctgaaggat
     5221 gagccgtaga gtggggcgca atagccgtaa cggtacccga ggtgggcgcg tgaacaggca
     5281 gcattttgcc gcgaccacgg gtaagcggct ggccgcgcaa tactttatcg ccgacgctaa
     5341 cgcacaactc accttcagcg ccaatatgct gtttcagtgg aatgacaaaa cgctgcgcca
     5401 gtggtacctg gcgcaggggt gtaccgttgg actgggtttt catctccggt ggatggatgc
     5461 cgccgttgaa atcccagatt ttattttttc tgaatgcaga gaataactta agcatggtgt
     5521 tccacgggaa tgatacgcac gggaatggtg ttcagatccc atttccagga gtcaggtgtt
     5581 tctgcgaccg gttgcaacga gatgcagtgc gtcgggcacg gatcaacaca taaattgcag
     5641 cccgtacaga gatcactcat taccgtatgc atggcacggg tagcgccaac gatggcgtct
     5701 accggacacg cctgaataca tttagtgcag ccaatacagt tattttcatc aataaccgcc
     5761 accatccgcg caggcgttag ctcttgcgct tcgccatcca gcggctgcgg ctcgacatta
     5821 agcaactcgg caatttttag catcacagct tcgccaccag gggcgcaacg gttgattttt
     5881 tcaccgttac agctgatggc ttccgcgtag gggcgacagc cgggataacc gcactgacca
     5941 cactggctct gcggtaagat ttcgtcaatt ttctcaacga ccggatcgtc ttccaccgca
     6001 aaacggcggg aggcataacc cagaatggcg ccaaacgcca ggcccagcag gctcacggcg
     6061 gcaacggcaa tccagatagc attcattaca acttcaccaa accactaaag cccataaagg
     6121 ccagagacat aagacctgcg gtaattaacg caatggcatt accgcgaaaa ggtgccggga
     6181 catcagccac agcaaggcgt tcgcggatgg ctgcgaagag aaccatcacc agcgaaaaac
     6241 cgacagcggc ggaaaaaccg tacagcgccg actgcaagaa attgtgcccg agattgatat
     6301 tcagcaacgc cacgccgagc acggcacagt tggtggtgat aagcggcaaa aaaatcccca
     6361 gcaaacggta aagcaccggg ctggttttgc gcaccaccat ctcggtgaac tgcacgacca
     6421 cagcaatcac cagaataaat gccagggtgc gcaggtaaat cagattaagt gggatcaaaa
     6481 tccacgtatc gataagccag gcgcaaatag acgccagcgt catcacaaac gttgttgcca
     6541 gccccatgcc catcgcggtt tccagctttt tggaaacccc cataaacgga cagagaccga
     6601 gaaacttgac cagtacaaag ttattgacca gtacagttcc gacaaacagt aacaggtagt
     6661 cagtcatttt tcggcctgaa ataaaaaagc cgcctattat ccgttaatcc agagcaggcg
     6721 acaacaggtt aactgtaagg ttattacgga ttcacaaacg tcgctttcac ccgcgacgag
     6781 cgtttgaaat aggggacgat caatgcggtt gccaacaaag taaacagcaa ctggcgaacg
     6841 gctatgccat cttccacggg tgaaaaagcg aacgctttta ccgccagcaa tacggaaata
     6901 agcagccaga taatatagtg tttaggaaca caacgccgac gtttaaagaa agcgatggtc
     6961 aaccacagtg tgtaatacca catggcgata gcggtaataa aagaaacgcc ccataagatt
     7021 tgtgtcgtca gtgcttgtgc gccgagcgtt tgaaatgttt gcggagaaga taacgcagcg
     7081 gtatacagca acaacgccag cgtcgtactt aacagagcga ctaaaagcca ggccaacggg
     7141 ccaagcaacc agcctccaat acgttgtggc gttgtggtgg tcataggttc tccagaatag
     7201 cgacgcaaac aggcaaatct aattttaggg gcgttattat aaggctttta taaccgatcg
     7261 ctactttctt tattgaacat agtgccagac ggacttcggt acctggccga ggtcaaacaa
     7321 acgcccgcca gaaaccagct ctgcgcgacg gtgatcggca gcacgataca tattgatcag
     7381 ttcctgatcg tccgtcaggg tgtaattaag atggtcgtag ag
//
LOCUS       DBFIFS010000217         7413 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.217, whole genome shotgun sequence.
ACCESSION   DBFIFS010000217 DBFIFS010000000
VERSION     DBFIFS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.217"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            53..289
                     /locus_tag="N7A07_004018"
     CDS             53..289
                     /locus_tag="N7A07_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2478584.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            279..1421
                     /locus_tag="N7A07_004019"
     CDS             279..1421
                     /locus_tag="N7A07_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2478585.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(1535..2785)
                     /gene="icd"
                     /locus_tag="N7A07_004020"
     CDS             complement(1535..2785)
                     /gene="icd"
                     /locus_tag="N7A07_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU2478586.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            2957..3610
                     /gene="rluE"
                     /locus_tag="N7A07_004021"
     CDS             2957..3610
                     /gene="rluE"
                     /locus_tag="N7A07_004021"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU2478587.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            3620..4081
                     /gene="nudJ"
                     /locus_tag="N7A07_004022"
     CDS             3620..4081
                     /gene="nudJ"
                     /locus_tag="N7A07_004022"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU2478588.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            4135..5241
                     /gene="mnmA"
                     /locus_tag="N7A07_004023"
                     /gene_synonym="trmU"
     CDS             4135..5241
                     /gene="mnmA"
                     /locus_tag="N7A07_004023"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU2478589.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            5277..5918
                     /gene="hflD"
                     /locus_tag="N7A07_004024"
     CDS             5277..5918
                     /gene="hflD"
                     /locus_tag="N7A07_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU2478590.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            5922..7292
                     /gene="purB"
                     /locus_tag="N7A07_004025"
     CDS             5922..7292
                     /gene="purB"
                     /locus_tag="N7A07_004025"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU2478591.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
BASE COUNT         1923 a         1895 c         1824 g         1771 t
ORIGIN      
        1 tcatgtcaga tatatcggtc acaaactccc tcagcagcta agaggaggac aaatgtctcg
       61 actaatcact ttacgggact gggctaaaga agaatttggg gacttagcac caagtgagcg
      121 agttctgaaa aaatacgcgc aagggaaaat gatggcccca cccgctataa aagttggtcg
      181 ctactggatg attgaccgaa attcccgttt tgtaggaacg cttgcagaac cgcaactccc
      241 aataaacgca aacccaaaac tccaacggat aatcgctgat ggctgctagc ccccgatctc
      301 acaaaatctc tatacccaat ttatattgca aattagataa gcgaaccgga aaggtatatt
      361 ggcaatacaa acatccacta tccggtcgtt ttcatagctt aggaactgat gagaatgaag
      421 caaaacaagt tgctactgaa gcaaatacca ttattgctga acaacgtacc cgacaaatat
      481 taagcgtcaa tgagcgtctg gaaagaatga aaggcaggcg ctcagacatt acggtgacag
      541 aatggcttga taaatataat tctatccagg aggacaggct gcaacataat gaactaagac
      601 ccaactccta tcggcaaaaa ggcaaaccca tccgtctttt ccgtgagcat tgtggaatgc
      661 aacacctcaa ggatattacc gcacttgata ttgccgaaat aattgatgct gtaaaggctg
      721 aaggtcataa caggatggcg caagtcgtga gaatggtgtt gatcgacgtc ttcaaagaag
      781 cacaacacgc aggacatgtt ccgccaggat ttaacccagc gcaggcaaca aaacaaccgc
      841 gaaatcgagt aaaccgtcaa agattatcac tgcccgaatg gcaggcaata tttgacagcg
      901 taagcagacg gcagccctat ttaaaatgcg ggatgctact tgctcttgtc actggacaac
      961 gtttaagcga tatctgcaat ttgaaattct ctgatatctg ggacgacatg ttgcacatta
     1021 ctcaggaaaa aaccggttca aaacttgcta ttccgcttaa cctgaaatgc gatgctctga
     1081 atattaccct tcgtgaagtt atatctcagt gcagggatgc tgttgttagt aaatatctgg
     1141 tccattaccg tcacactacc tctcaagcaa acagaggaga ccaggtgtct gcaaatactc
     1201 ttacaacggc ttttaaaaag gccagggaaa aatgtggcat aaaatgggag caaggaactg
     1261 cgcccacatt tcatgagcag cgatctctgt cagaacggtt atatcgggaa cagggtctgg
     1321 atacgcaaaa gttgttaggt cataaatcca gaaaaatgac cgaccgatac aatgatgatc
     1381 gtggtaaaga ctggattatc gtagatatca aaacagcata gaaaatagcc agttttgggg
     1441 aagggttttg gggaaagttt tggggaagat tttaaatcat cataaaacaa cgggcgtgtt
     1501 atacgcccgt ttcaatattt aacacatgta gtgattacat attcttgatg atcgcgtcac
     1561 caaactctga acatttcagc agtttagcgc cttccatcag acgttcgaag tcataggtta
     1621 cggtcttggc attgattgcg ccttccatac ctttaacaat caggtcagcc gcttcagtcc
     1681 aacccatatg gcgtaacatc atctcagcag agagaataat agaacctggg ttaactttgt
     1741 cctgaccggc atatttcggc gcagtaccgt gggtggcttc aaacagggcg cattcgtcac
     1801 cgatgtttgc gccaggggcg ataccgatac cgccaacctg cgctgccagg gcgtcagaaa
     1861 tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga cgcagcagga
     1921 tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg ccggtgttcg
     1981 ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac tcttcacgcg
     2041 ccagctcgta gccccagtct ttaaacgcgc cttcggtgaa cttcatgatg ttgcctttgt
     2101 gcaccagagt cacagagtca cgatcgttag caattgcgta ttcgatcgct gcacgaacca
     2161 gacgtttggt gccttcttca gaacacggct taataccgat accgcaatgt tccgggaagc
     2221 gaattttctt cacgcccatc tcttcacgca ggaatttaat cactttctcg gcgtcggcag
     2281 agtcagcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg aagataacca
     2341 tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ctgatagtaa cgtaccggac
     2401 gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag cgaataccgc
     2461 caccaaccgg agtggtcagc gggcctttaa tggcaacgcg atattcacga atcagatcaa
     2521 gggtttcagc aggcagccag acgtcctgac cataaacctg tgtggatttt tcaccggtgt
     2581 aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact gcagcgtcga
     2641 ccactttcag catggctggg gttacatcta caccgattcc atcaccttca atgtaaggga
     2701 taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc ttgccttgtg
     2761 ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg tttgctcgtc
     2821 tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga gcaatttttt
     2881 gttaatgatt tgtaattggc ttgtcaatac taccctattg tttgccgtca tgaaagatgc
     2941 gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc aaaaaacttc
     3001 ttttagaaat caccaggtta agcgattcag ctcacaacgt tctaccaggc gtaaacctga
     3061 aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc cgcagttcac
     3121 cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg tttatgcagc
     3181 aggtcgtctt gaccgcgata gcgaagggtt actggtgctg accaataacg gcgcgttgca
     3241 ggcgcgttta acccagccgg gcaaacgcac cggaaaaatc tattatgtgc aggtggaagg
     3301 tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa atgatggccc
     3361 taccctgccc gcaggcgcgg agctggttga cgaacccgcg tggttatggc cgcggaatcc
     3421 accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct tatatgaagg
     3481 acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc tgcgactgat
     3541 tcgctatgcg atgggtgatt actctttgga taaccttgcc aatggcgaat ggcgagaagc
     3601 aacagaataa ggatgcacaa tgtttaaacc gcacgttacc gttgcttgcg tagtgcacgc
     3661 agaggggaaa tttttagtcg ttgaagagac gattaatggt aaagccttat ggaaccaacc
     3721 tgccgggcat ctggaagccg atgaaacctt agtggaagca gccgcccgtg agctgtggga
     3781 agaaaccggc atcagcgcgc agccgcaaca ctttattcgt atgcatcagt ggattgcgcc
     3841 agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa tatgcccgac
     3901 ccagcctcat gacagcgata tcgactgctg tcgttgggtc agcgccgaag aaattttaca
     3961 ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc aaagcgggca
     4021 acgctatccg ctggagatga ttggcgattt taactggcct tttacaaagg gtgtcatcta
     4081 aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga tccaatgtct
     4141 gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc cgtttctgcc
     4201 tggctgttgc aacaacaggg atatcaggtc gaaggcctgt ttatgaagaa ctgggaagaa
     4261 gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca ggctgtctgc
     4321 gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg ggacaacgtc
     4381 ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga tattctgtgc
     4441 aacaaagaga tcaaatttaa agccttcctc gaatttgccg ccgaagattt aggtgccgat
     4501 tatatcgcta ccggtcatta cgttcgtcgg gcagatgtcg atggcaagag ccgcctgctg
     4561 cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag ccatgagcag
     4621 attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg taagattgct
     4681 gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg cttcattggc
     4741 gaacgtaaat tccgcgagtt ccttggccgt tatctcccgg cgcaaccggg caaaatcatt
     4801 acagtcgatg gcgatgaaat tggtgagcat caggggctga tgtatcacac cctcggtcag
     4861 cgcaaaggtc tgggtatcgg tggcaccaaa gaaggcaccg aagaaccgtg gtatgtggtc
     4921 gataaagacg tcgaaaacaa cattctgatt gttgcgcagg gccatgaaca cccgcgtctg
     4981 atgtctgtcg gtctgattgc ccagcagctg cactgggtcg atcgtgaacc attcaccggt
     5041 actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccctg caccgtcaag
     5101 gcgctggacg atgatcgcat tgaagtgatt ttcgatgagc ccgttgccgc cgtgacgccg
     5161 ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat tattgagcag
     5221 cgtctgccgc tgccgatctg attattatct ttacttaaaa caaggaagca gtgaacgtgg
     5281 caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg gcacgcctgg
     5341 tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc tcactcaaca
     5401 gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc gaagccaacc
     5461 tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc cagggcttaa
     5521 acgccgaatt aacccgctac acactcagcc tgatggtgct taagcgcaaa ctctcctcag
     5581 cgaaaggtgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc cagctcgaac
     5641 acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat gttgatgtga
     5701 tcagcccgct tggcccgcgc attcaggtca ccggttcccc tgctgtactg caaagcccac
     5761 aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc gtgctctggc
     5821 accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg accactcagg
     5881 caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta tcctcactga
     5941 ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg cgcgggattt
     6001 tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg ctgcaaaaac
     6061 tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca atcggttacc
     6121 ttgatgcaat tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa accatcgagc
     6181 gtactactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa gtggcggaga
     6241 tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg gaagatatca
     6301 ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc ctgccgtact
     6361 ggcgtcaact aattgatggc attaaagatc tcgccgttca gtatcgcgat atcccgctgc
     6421 tgtcccgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag atggcaaacg
     6481 tcgcctaccg tatggagcgc cagtaccgcc agcttaacca ggtggagatc ctcggcaaaa
     6541 tcaacggcgc ggtcggtaac tataacgccc acatcgccgc ttacccggaa gttgactggc
     6601 atcagttcag cgaagagttc gtcacctcgc tgggtattca atggaacccg tacactaccc
     6661 agattgaacc gcacgactac attgccgaac tgtttgattg cgttgcgcgc ttcaacacca
     6721 ttctgatcga ctttgaccgt gacgtctggg gttatatcgc ccttaaccac ttcaaacaga
     6781 aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac ccgatcgact
     6841 tcgaaaactc cgaagggaat ctgggccttt ccaacgcggt attgcagcat ctggcaagca
     6901 aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg cgtaacctcg
     6961 gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc gtgagcaaac
     7021 tggaagtgaa ccgtgaccat ctgctggatg agctggatca caactgggaa gtgctggcag
     7081 aaccaatcca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag aagctgaaag
     7141 agctgactcg cggtaagcgc gttgacgccg aaggcatgaa gcagtttatc gacggtctgg
     7201 cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggctaac tacattggtc
     7261 gcgccatcac catggttgat gagctgaaat aaactcgtac cagtgccgga tggcgatgct
     7321 gtccggcctg cttattaaga ttatccgctt tttatttttt cactttacct cccctccccc
     7381 gctggtttat ttaatgttta cccccataac cac
//
LOCUS       DBFIFS010000218         7300 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.218, whole genome shotgun sequence.
ACCESSION   DBFIFS010000218 DBFIFS010000000
VERSION     DBFIFS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.218"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            75..5503
                     /locus_tag="N7A07_004026"
                     /pseudo
     CDS             75..5503
                     /locus_tag="N7A07_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5619..6683
                     /gene="hipA"
                     /locus_tag="N7A07_004027"
                     /pseudo
     CDS             5619..6683
                     /gene="hipA"
                     /locus_tag="N7A07_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            6954..7223
                     /locus_tag="N7A07_004028"
                     /pseudo
     CDS             6954..7223
                     /locus_tag="N7A07_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1963 a         1540 c         2050 g         1747 t
ORIGIN      
        1 ttaataaatt tcatttttaa tgtctgacga ctcatctctt tcgtggctgt attgtttgta
       61 tttggagcgt ttatatgaat agaatctatc gcgtgatatg gaattgcact ctacaggtat
      121 ttcaggcctg ctcggaatta actcgcaggg taggtaaaac atcaacggtt aatttgcgaa
      181 agtcctctgg actgacaacg aaattcagta gattgacgct gggtgttttg ctggcactaa
      241 gcggttcagt gtctggtgcg agtctggaag ttgataatgg tcagattacc aatattgata
      301 ctgatgttgc ttatgatgcc tacctggttg gctggtatgg cactggagtg cttaatattt
      361 tggctggcgg taatgcctcc ttaaccacta ttactaccag cgtcattggc ggtaatgagg
      421 attcggaggg taccgttaat gttttgggtg gcacctggcg attgtatgat agcggaaata
      481 atgcaaggcc tttaaatgtg ggtcaatccg gaacggggac gctgaatatt aaacagaagg
      541 gtcacgtcga tggaggctat ttaagattag gtactcaagc tgcaggcgtc gggacggtta
      601 atgttgaggg agaggactcg gttttgacga ccgaattatt cgaaataggg agttacggta
      661 cgggctcatt aaatattacg gataagggtt acgtcacgag ttcaatagtc gccattttag
      721 gatatcaagc gaacagtaat ggtaaggttg tcgttgaaaa gggtggcgag tggctaataa
      781 aaaataatga ttcctcaatt gaatttcaaa ttggtaatca aggaactggg gaggcgacta
      841 ttcgcgaggg tgggctgatt acggctgaaa atacgattat cggtggcaat gccaccggtg
      901 tcggaaccct gaatgtgcag gatcaagact ctgtcatcac ggtacgcaga ctctataatg
      961 gatatttcgg taatggcgca gtcaatattt ccaataatgg actaattaat aacaaagaat
     1021 attcattggt gggcgttcag gacggttccc acggtgtcgt caacgtgacc gataaagggc
     1081 attggaattt cctcggaacg ggcgaagctt tccgctatat ctatatcggt gatgctggcg
     1141 acggtgaact taatgtctcg cgtgaaggta aagtagattc gggaattatc actgcgggga
     1201 tgaaagaaac aggcacaggc aaccttactg ttaaggataa gaactccgtt atcactaatc
     1261 tcggaactaa tcttggttat gacggccacg gcgaaatgaa tatcagtaat gaggggcttg
     1321 ttgtcagcaa cggaggaagc tctctcggtt atggagaaac cggcgtcggg aaggttagca
     1381 tcaccacggg ggggatatgg gaggtcaata agaatgtcta taccaccatt ggtgttgcgg
     1441 gcgtcggaaa cctcaatatt agcgatggcg gtaagttcgt atcgcaaaat attacttttt
     1501 tgggcgataa agcaagcggt atcggcacac tgaacctgat ggatgcgaca tcgtcgttcg
     1561 atactgtggg tatcaatgtc ggtaattttg gtagcggtat cgtaaatgtc agtaatggtg
     1621 ccacccttaa ttcaacgggc tatggattta tcggaggaaa tgcctccggt aagggaatag
     1681 ttaatatttc aacggatagt ctctggaatt taaagacgtc ttctactaac gcccaattgc
     1741 tacaggtcgg tgtattaggc acgggtgaac tgaatattac caccggaggt atagttaaag
     1801 cgcgtgatac acagatagct ctcaatgaca aaagtaaggg cgacgtgagg gtggatgggc
     1861 agaactctct tcttgaaaca ttcaatatgt acgtagggac atctggtacg ggtacgttaa
     1921 ccctgacgaa tagcggcacg ctgaatgtcg aaggtggaga agtttactta ggtgtttttg
     1981 aacctgctgt aggaacgcta aacattggtg cagctcacgg tgaggcggcg gcagatgccg
     2041 gatttatcac caacgcgacg aaagtagagt ttggctctgg cgaaggtgtt tttgtcttta
     2101 atcatactaa taacagtgat gccggctacc aggtcgatat gctgattaca ggtgacgata
     2161 aagacggaaa agtgatccat gatgcaggcc atacggtgtt caatgcaggg aatacttata
     2221 gcggtaaaac gctggtcaat gacggcctcc tgaccattgc gtctcatacg gcagatgggg
     2281 taacgggcat ggggtcgagt gaagtaacca ttgcaagccc cggtacgctc gacattctcg
     2341 catcaacgaa cagtgcagga gattacacgc tgaccaatgc gctcaaaggc gatggcttga
     2401 tgcgagttca gctgtcatcc tccgacaaga tgtttggctt tacacatgca acagggactg
     2461 aattcgccgg tgttgcccaa ctgaaagaca gtaccttcac tctggaacgc gacaacaccg
     2521 ctgcgcttac tcacgcgatg ttgcagtctg acattgaaaa taccacatcg gtaaacgtgg
     2581 gagagcaatc cattggtgga ctggccatga atggcggtac gctcattttc gatacggata
     2641 ttcctgctgc gacgcttgca gagggatata tcagcgtcga tacgctggtt gtcggcgcga
     2701 gtgactacac ctggaaaggc cgtaactatc aggtaaacgg gacgggcgac gtgcttatcg
     2761 gcgtgcctaa accgtggaat gatcctatgg cgaataaccc tctgacgacg ctcaatttgc
     2821 tggaacacga tgataaccat gtcggcgttc aactggtgaa ggcgcaaacg gttattgggt
     2881 cgggtggctc attaacgtta cgtgatttac agggcgacga ggtggaagcg gacaaaacgt
     2941 tacacattgc gcaaaacgga acggtggtcg ccgagggtga ttatggattc cgcctcacga
     3001 ccgcaccagg tgatggtttg tacgttaact atgggctgaa agcgctgaac atccatggag
     3061 ggcaaaagct gacgttagcc gaacatggcg gagcctatgg cgcaacggcc gatatgtcgg
     3121 caaaaatcgg tggtgaaggg gatctggcaa tcaatacggt gcgacaggtt tcgctttcca
     3181 acggtcagaa cgactatcag ggggcaacct acgttcagat ggggacatta cgtaccgatg
     3241 cggatggcgc gctgggcaac acccgggaac tgaacatcag caacgcagcc atcgtcgatc
     3301 ttaatggatc gacgcagacg gtagagacat tcaccgggca gatgggttcg actgttttgt
     3361 tcaaagaagg gtcgctgacg gtaaataaag gtgggatcag tcagggtgaa ctgacaggtg
     3421 gcggaaacct gaatgttaca gggggaacgc tggctgtcga ggggcttaat gcacgctaca
     3481 atgcgttaac cagcgttagc ccaaatgcgg aagtcagcct cgataatacg caggggttag
     3541 gcagaggaaa tattgccaat gacggtctgt taacgctaaa aaacgtgact ggcgaactgc
     3601 gtaatagcat aagcgggaag ggtatcgtga gcgcaaccgc caggacagat gtagagctgg
     3661 atggcgataa tagccgcttt gtggggcaat tcaacattga tacaggcagc gcgctcagcg
     3721 tcaacgagca gaaaaacctg ggtgatgctt ccgttatcaa taatggcctg ctcaccatct
     3781 ccactgagcg tagctgggcg atgacgcaca gtatcagcgg tagcggtgat ttgacaaaac
     3841 tgggtaccgg gatcctgact cttaacaacg attcctcggc gtatcagggt acgacggata
     3901 tcgtgggggg ggaaattgct ttcggttccg actctgccat taatacggca agtcaacaca
     3961 ttaatatcca taacagcggt gtgatgtcgg gaaatgtcac cactgcaggt gatgtgaacg
     4021 ttatgtctgg ggggacactg cgtgtcgcta aaaccacaat cggcgaatcg gcggcaactt
     4081 ggagaatggc ggcacggttc aaatgaacag tgaaggagga aaaccgggga atgtactgac
     4141 cgttaacggc aactataccg gaaacaatgg cctgatgacg ttcaacgcga cgctgggcgg
     4201 cgataattca cccaccgata agatgaacgt gaaaggcgat acccaaggga acactcgcgt
     4261 tcgggttgat aacattggcg gcgtcggtgc gcaaacggtc aacggtattg aactcattga
     4321 ggttggcggt aattctgcag gtaatttcgc gctgaccacc ggaactgtcg aagctggggc
     4381 ttacgtctac acgctggcta aagggaaggg gaatgacgag aaaaactggt atctgaccag
     4441 taaatgggac ggcgtaacgc cagcggatac acccgatccc atcaataatc cccctgttgt
     4501 ggatccggaa ggcccatcag tttatcgccc ggaggccgga agctatatca gcaacattgc
     4561 cgcagccaac tcgctgttta gccatcgctt acacgaccgt ctgggtgagc cgcaatatac
     4621 agattcactg cattctcagg attcagcaag cagtatgtgg atgcgtcatg tcggggggca
     4681 cgaacgttcc agtgccggag acggccagct aaatactcag gctaaccgct atgtattgca
     4741 gctaggcggc gatttggcgc agtggagtag caacgcgcag gatcgctggc atcttggcgt
     4801 gatggcaggc tacgccaatc agcacagtaa tactcagagt aatcgtgtgg gttataaatc
     4861 ggatgggcgc atcagcggtt acagcgctgg gctgtacgcg acctggtatc agaacgatgc
     4921 gaataagacc ggcgcttatg ttgacagctg ggcgctgtat aactggtttg ataacagcgt
     4981 cagttccgat aaccgttctg ctgacgacta tgattctcgc ggtgtgacgg cctctgttga
     5041 gggtgggtat acctttgaag cgggaacatg tagcggcagc gaagggacgc tgaatacctg
     5101 gtacgtccag ccacaggcgc aaatcacctg gatgggtgtg aaagattctg accatgcccg
     5161 gaaagacgga acgcgcattg aaacggaagg cgacggaaac gtgcaaacgc gacttggggt
     5221 gaaaacctac ctgaatagcc atcaccagcg tgacgatggt aaacagcgtg agttccagcc
     5281 ttacattgaa gcgaactgga tcaacaatag caaagtctac gccgtgaaga tgaatggtca
     5341 aaccgtaagc cgtgatggtg cgcgaaatct cggtgaagta cgtaccgggg ttgaggcgaa
     5401 agtaaataac aaccttagcc tgtgggggaa tgtcggtgtg caactaggtg ataaaggcta
     5461 tagcgatact cagggcatgc tgggagtgaa atatagctgg taaacagtat aagccgcatg
     5521 tcgagatggc atgcggctta atattgccaa cttcaaaagg cttttaaagc ctccctttta
     5581 tccgcgatcg cggatatcgc agcgtttatc ccgtacagtt tgatttattg tcagaaatag
     5641 ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga cgaaaccgta acgcgtccga
     5701 taatggcatg ggaaaagctt actgaagtca gacttgaaga agtattaacg gcttataaag
     5761 cagatatccc gctaggcatg attagagaag aaaatgactt tcgcatctcg gttgctggcg
     5821 cacaggagaa gacagcactg ctcagaatag gcaatgactg gtgcattccg aaaggaataa
     5881 cgccgacgac gcacatcatt aaattaccga ttggggaaat caggcagccc aatgcgacgc
     5941 tcgatctcag ccaaagcgtt gataatgagt attactgtct gctgctggcg aaagaacttg
     6001 ggttgaatgt cccggacgca gaaatcatta aagcgggaaa tgtgcgcgcg ttagcggtcg
     6061 aacgttttga caggcgttgg aatgctgagc gaacggtttt acttcgcttg ccacaggagg
     6121 atatgtgtca gacattcggt ttaccttcat cggtgaaata tgaatcagat ggaggcccag
     6181 gcatcgcgcg gatcatggct tttttgatgg ggttcagcga ggcgcttcgc gatcgttatg
     6241 attttatgaa attccaggtc ttccagtggt tgattggcgc aacggatggc catgcaaaaa
     6301 acttctccgt atttattcag gctggcggca gttatcgact cacgccattt tacgacatca
     6361 tttcagcatt tccggtcctt ggcggtacgg gaatacacat cagcgatctc aaactggcaa
     6421 tggggcttaa cgcatccaaa ggcaaaaaaa cggcaatcga taaaatttat ccgcgacatt
     6481 ttttggcgac agcaaaggtg ctgagattcc cggaagtgca gatgcatgaa atcctgagtg
     6541 actttgccag aatgattccg gcagcactgg ataacgtgaa gacttcatta ccgacagatt
     6601 ttccagagaa cgtggtgacg gcagttgaaa ccaatgtgtt gaggttgcac ggtcggttaa
     6661 gccgagaata cggtattaag taagagtaag atatggggtt tggtcgttgt taatgagcag
     6721 gacaatcatg accgccaacg actagattct cccttggttt atattggtgc gcataatgta
     6781 tattatgtta aatcgtatct gagtgatacg aagtgatttt ccggtagtcg ttattcgttc
     6841 aggattccag aacttaagct tctcctcttt cataattggc ctgtgcatga cggatatagg
     6901 ctggctggta tgccgatttg ccgtttgccg catcttccca gcgttgaaga ctcaagctgg
     6961 caaaccgcgt gcaaatgagc acgccctctt tccatcaaca cttcaggcag cttgcgggaa
     7021 tgagtccact ccggtatcag aaatggctac gtctcaatga agtcaggcga acgatgctga
     7081 atgaacatta cgatgtcacc acagcagcct atgctgtcgg ttacgaaagc ttatcccatt
     7141 tccgacggga atatttgcgg atgtttggag agtcacctaa gagagatatt accaggttaa
     7201 gaaagtctgt cggtcagctt taataaaccc tgcacttatc tgtttagagc aattcggtgt
     7261 tagttacacc aggcaaacat taactatagc taatgattta
//
LOCUS       DBFIFS010000219         7248 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.219, whole genome shotgun sequence.
ACCESSION   DBFIFS010000219 DBFIFS010000000
VERSION     DBFIFS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.219"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(126..830)
                     /locus_tag="N7A07_004029"
     CDS             complement(126..830)
                     /locus_tag="N7A07_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU2478592.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(966..1418)
                     /gene="moaE"
                     /locus_tag="N7A07_004030"
     CDS             complement(966..1418)
                     /gene="moaE"
                     /locus_tag="N7A07_004030"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU2478593.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1420..1665)
                     /gene="moaD"
                     /locus_tag="N7A07_004031"
     CDS             complement(1420..1665)
                     /gene="moaD"
                     /locus_tag="N7A07_004031"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU2478594.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVSGG"
     gene            complement(1658..2143)
                     /gene="moaC"
                     /locus_tag="N7A07_004032"
     CDS             complement(1658..2143)
                     /gene="moaC"
                     /locus_tag="N7A07_004032"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU2478595.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2146..2658)
                     /gene="moaB"
                     /locus_tag="N7A07_004033"
     CDS             complement(2146..2658)
                     /gene="moaB"
                     /locus_tag="N7A07_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU2478596.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2680..3669)
                     /gene="moaA"
                     /locus_tag="N7A07_004034"
     CDS             complement(2680..3669)
                     /gene="moaA"
                     /locus_tag="N7A07_004034"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU2478597.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3657..3801)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4066..4974
                     /gene="yvcK"
                     /locus_tag="N7A07_004035"
     CDS             4066..4974
                     /gene="yvcK"
                     /locus_tag="N7A07_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU2478598.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5165..7186)
                     /gene="uvrB"
                     /locus_tag="N7A07_004036"
     CDS             complement(5165..7186)
                     /gene="uvrB"
                     /locus_tag="N7A07_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU2478599.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1726 a         1938 c         1835 g         1749 t
ORIGIN      
        1 atggatacgg aaaactcttc cgctcggcca taaggcgtat tttatacctg gtacttgtta
       61 agtgtcgttt ttagtaagaa aaaaaatccc gacacatggc cgggatattg atggtgaaaa
      121 aagaattaac gacgattgcc aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc
      181 agatacaagg ttaatgcgca aagaatggaa tatttgcgca ggttcgacgc gtcgcgggta
      241 tctatctgct cacccatatt tttcagtttc tgggtgtcat acgccgtcaa tccgacaaag
      301 acaatcacgc cgatgtaggt gactgcccac atcaacgctt cgcttttcag ccagaagttg
      361 accagcgatg ccaacacaat gccgattaac gccataaaca gcatattgcc aaagccactt
      421 aaatcgcgct tcgtggtgta accgtacagg ctcattgcgc cgaacatccc ggcagtaacg
      481 acgaaggtac tggcgataga agcagcggta taaacaatga aaatactgga aagcgtaaga
      541 cccgtcagcg ccgaataaag cataaagagc atcgtcgtta cacctgcgct cagcttttga
      601 atcatcgctg ataacacaat aaccaatgct aattgcgcga tgatcagacc gattaaaaag
      661 acacggttag tgaacaacag ctccatcacg gccgcggaat tagccgcata ccaggcaaca
      721 aatgcggtca gcaataagcc aacggtcatc cagccataga cttgcgccat ataagtctgc
      781 aagccagccc ggggttgtac aattgaatca gaacgtggga atctgtccat gacgatctcc
      841 tgaagatata aggaatatct taaggatact gcaaaatgat gaggctgtgc atcgacgcag
      901 cgtaaacgca tgtactgagc ggtgaaattg ccggacgcag cggtgcctta tccggctaac
      961 aaaaactacc agcgttttgc cgcctgctga tcgctctccc gagcttcgac ccagcggtcg
     1021 ccctccggcg tggcttcgcg cttccagaac ggtgcgcggg ttttgagata atccataata
     1081 aactgcccgg cttcaaacgc actgctgcga tgcgcactgg tgacaccgat aaaaacgatt
     1141 tcatcgcccg gccataattc cccgatgcgg tgaatcaccg tgacgcgccc cagcggccag
     1201 cggttacgcg cttcatcgac aatttctgcc agtgcttttt cagtcatccc cggatagtgt
     1261 tcgagggtta atgctttgac gctgtcgccc aggttatggt tgcgcacctt accggtaaag
     1321 gtgactaccg caccgtcttc gtcacgctcc gccagccacg ggtactcttc tcctacgctg
     1381 aacggctgcg gaccaacaac aattttggtt tctgccatct taacctccgc ttaccggcgg
     1441 gaagaaagct acttcgtcgc cgtcagtcag cggatggtca aaactcacca gcgtctggtt
     1501 gacggcagcc agtaatttgc catcttccag cgccagcgcc cagcgatcgc tctgcgcagc
     1561 caggtgctgg cgtaacgctt caacggttgg gaaatccgca gccacttcgg ttgcatctgt
     1621 tcctaccaac tcgcgcacct gggcgaaaaa aagaacttta atcatccgct tccaccttaa
     1681 agtcacccga cttgccgccg ctcttcgcca gcaaacgtac cggaccaatc accatatctt
     1741 tttgcaccgc tttgcacatg tcataaatgg tcagcgccgc cacggaggcc gcggttaacg
     1801 cttccatttc gacaccggtt ttcccggtca ggcggcataa ggtttctata cgcacccgat
     1861 tgtgctccgg ctcggcctgt aaattgactt caactttgct gagcatcagc ggatgacaca
     1921 gcgggatcag atcccaggtg cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa
     1981 atacgtcgcc tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg
     2041 tgacaaaggc ttccgcccgc gcttcacgca cggtttccgc tttggcggag acatccacca
     2101 tgtgcgcttc gccagcggcg ttgatatggg tcagttgcga catacttatt tcttcaaatg
     2161 tggatggaaa ttacacggac gcgtacgggc atccagctgc ggcgcgatga tattttccca
     2221 tgcggtacgg cacgctttgg tcgaacccgg catggcgaaa atcagcgttt tgttagcgac
     2281 gcccgctacc gcacgagatt gcaacgtgga agtgccaatc tcttcaaacg acaacatacg
     2341 gaacacttca ccaaaacctt caacttcacg gtcgaacaac ggcagcaatg cttcgggagc
     2401 ctgatcacct tccgtcaggc cagtcccccc cgtaatcaac accacttgta catcgtcgct
     2461 ggcgatccac gcagatacct gagcgcgaat agcgtagcgg ttttctttca caatggcttt
     2521 atcgacaacg tgatggcccg cttcttgcgc cgaatcgcgc aaatagtgac cggaggtatc
     2581 gtcttcttca ccgcgacgat tagaaaccgt aagaatagca atacgggtcg ggataaattc
     2641 agtgcttacc tgactcatct gatctctcct tttgacgttt tagccgccaa tgtacgataa
     2701 gttttgcgta ataccggtgt tgttttgatg caggaaatgg gtctgtttct tctcccgcag
     2761 cgccgctgaa atacgcgctt ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg
     2821 caggttaacg ccgccttcac caaacaggca gagatggagt ttaccaatgg aggaaacgcg
     2881 cagacggttg caagtggcgc agaagtcttt ttcatacggc atgataaggc caatctctcc
     2941 ggcgtaatcc ggatgacaaa agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg
     3001 gatccagccg cgacgcagta gctcgtcacg cagaacctga ccagagatgt gatgcttacg
     3061 gaaaagctcg ctgccctcgc ccgtttccat cagttcgatg aaacgcagct ggatagggcg
     3121 atgctggatc cagttcagaa aggtgtcgag ctggtgatga ttcacatcac gcatcagcac
     3181 ggtattgact ttgaccttct caaaaccggc ctcaaatgca gcatcgatcc ctgccatcac
     3241 ctgattgaat ttatcctggc cggtaatggc atgaaactgg cgggcgtcca gactgtcgac
     3301 gctgacgtta atgccagtaa gtcccgcatc gcgccagttc gccacatcgc gttccagacg
     3361 gtaaccattg gtggtgaccg ctatctggcg aatagcgtcg ttttctcgca cagcggcgat
     3421 gatatcggta aagtcgcggc gtaaagacgg ctcaccgcct gtcaggcgca ctttttcggt
     3481 gcccagactg gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtca gaaagccttt
     3541 attggtgacg ccgctcggtt tgtagccatc cggcaggcag taggtgcaac gaaagttaca
     3601 cacatcggta atcgacaggc gcaagtagta aaacttacgc gcaaatgcat cagtcagttg
     3661 tgaagccatg tacacctttc cagatacggg aggcgaagtc atttcttcct tcgccctggt
     3721 ggcaatcttt acgagcattg tcacggccaa agcaccatat cagttgaccc aggtgcagag
     3781 gctagagtgt ttagtggtta agccgatact agcgtgtaaa tgagattttt accatccaca
     3841 ctttcgctat gtaatcatgt atatagcgtc ctgatcgtgc tatttcgcta cagaataagg
     3901 gaaaatggcg gttttacttt gatatacatc atcagggagg catagcgtgc atcgtccttt
     3961 agaggtagaa gaaaatagtt tgtatcctca atattggcag gttaattgct gtttccccgc
     4021 aatttgcgct actgtagcgc ggataatctg actccaggaa actatatgcg caatcgtacg
     4081 ctggctgatc ttgatcgtgt cgttgctctc ggcggagggc atggactggg acgcgttctc
     4141 tcatcacttt cgtctttggg ttctcgttta acgggtatcg tcaccaccac ggataatggt
     4201 ggctcgacgg ggcgtattcg ccgttcagaa ggcggcattg cctggggcga tatgcgcaac
     4261 tgccttaacc agctgataac tgaacccagc gtcgcttcag caatgtttga atatcgtttc
     4321 ggcggtaatg gcgaactttc cgggcacaac cttggaaacc tgatgttgaa ggcactggat
     4381 caccttagcg tgcgacctct tgaagcgatc aatctgattc gaaatctgct gaaagtggac
     4441 gcacatttga ttccgatgtc ggagcatcct gttgatctga tggcgattga cgatcagggg
     4501 catgaagttt acggcgaggt caatatcgac cagttaacta cgccgattca agagttattg
     4561 ttaacgccta atgtacccgc aacgcgtgag gcggttcacg ctatcaatga agcggatctc
     4621 atcattattg ggcctggcag tttttacacc agcctgatgc caattctgct gctgaaggaa
     4681 atcgcgcagg cattacgccg cacgccagcg ccgatggtct atatcggcaa tctggggcgt
     4741 gagttgagtt tacctgcggc caatttgaag ctggaaagca agctggcaat tatggagcag
     4801 tatgttggta aaaaggtcat tgatgcggtc atcgtcgggc caaaagtgga tgtctcggcg
     4861 gtgaaagagc ggattgtgat tcaggaggtg ctggaggcca gcgatattcc gtatcgtcat
     4921 gaccgccagt tgttacatag cgcactggaa aaggcgttac aggctttagg ttaacggaaa
     4981 tccgaaggaa aattccggct tcctaatgaa gacaaagtgc gcgttgttta tgacggatgc
     5041 ggcgtgaacg ccttatccgg cctacaaacc gcgctaattc aatgtattgc agaatcatgt
     5101 aggcctgata agcgtagcac atcaggcaat tttgcttttg tcatctgtct tcttcgttat
     5161 cctgtcacga agccgcgata aacaattcac gcagctgatg caactggtca cgaatttgtg
     5221 ccgcttcttc gaactccaga ttttgcgcgt gttgcatcat taacccttcc agctcatgga
     5281 ttttctgttg caacgcttta ggcgacatat ccatcggcac attatccggc tcaacaatcg
     5341 gtcgcgattt tcctctgccc ttcgctttgg ttttagcaat gttctgcccc aacgccagga
     5401 tatcgaccac tttcttgttc aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct
     5461 gctgtttctc gcggcgacgt tcggtttcgc caatcgcttt cgccattgac ggggtgatct
     5521 tatcgccgta gagaatcgct ttaccgttaa cgttacgcgc cgcacgacca atagtctgga
     5581 tcaacgaacg ttcagaacgc aggaagcctt ctttgtcggc gtcgaggatc gccaccagcg
     5641 aaacctccgg catatccaga ccttcgcgca gtaagttgat ccccaccagc acgtcgaact
     5701 cgcccagacg caagtcgcgg ataatctcca tacgttcgac ggtgtcgata tctgagtgaa
     5761 gataacgcac gcgctcaccg tgttcttcga gatattcggt aagatcttcc gccattcgct
     5821 tggtcagtgt ggtgaccagt acgcgttcgt taattgccgc tcgctgacga atctccgaaa
     5881 gaagatcatc aacctgtgtc gccactggcc gcacttcgat aatcggatca agcaatccgg
     5941 ttggacgcac cacctgatcc accacatcgc cgccggattt ttccagctca taattacctg
     6001 gcgtcgccga aacatagatg gtttgcggcg ctaatgcttc gaactcttca aatttcagcg
     6061 gacggttatc cagcgctgat ggcaggcgga agccgtactc caccagtgtc tctttacgcg
     6121 cccggtcacc gcgatacatg ccgccaattt gtggaatggt gacgtgagat tcatcgacca
     6181 ccagcagccc atcggcaggc aggtaatcaa acagcgtcgg cggtggctca cccggtccac
     6241 gaccggagag gaagcgcgag tagttttcaa tccccgaaca gtagcccagc tcgttcatca
     6301 tctccagatc aaactgggta cgctgggtca gccgctgctc ttccagcagt ttgttgtttt
     6361 ccaatagcac tttgcgtctg gcggccagct cttctttgat ctcctccatc gcctgtacga
     6421 tgcgctcgcg cggtgtgacg tagtgcgttt tcgggtagat ggtaaaacgt ggaatggtgg
     6481 aaacaatctg cccggttagc gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca
     6541 gttccacgcg aagtgcaatg tcatccgatt ctgccgggaa gatatcaatc acctcgccac
     6601 gaacacggaa agtaccacgc tggaatgctt gatcattacg agcgtattgc agctccgcca
     6661 gtcggcgcag aatcgcgcgc tgatcgataa tcataccgac cgtgagatgg agcatcatct
     6721 tgagatataa atcaggatcg cccagaccat aaatcgcgga aacagacgcg accacaacaa
     6781 catcacgccg ctccagcatc gctttggtgg cggacaaacg catctgttcg atatgttcgt
     6841 ttaccgaggc atctttctca atgaaggtgt cggaactcgg tacataggct tctggctgat
     6901 agtagtcgta gtaggagacg aaatattcca ccgcgttttc cgggaagaac tctttcattt
     6961 cgccatacag ctgggccgcc agcgttttgt tgggcgcaag taccatggta gggcgctgaa
     7021 ggtcagcaat gacattggca atggtgaagg ttttcccgga accagtcaca ccaagtaacg
     7081 tctggtgcgc caggccatct tccagcccct cttcgagacg tcgaatcgcc tctggctgat
     7141 cgccagaagg tttaaaagcg gaattcagtt tgaacggttt actcatgagt cgctacctga
     7201 aggagttggg cgggcaggta tgtaatttta ctcgtcgtac ttaattat
//
LOCUS       DBFIFS010000220         7226 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.220, whole genome shotgun sequence.
ACCESSION   DBFIFS010000220 DBFIFS010000000
VERSION     DBFIFS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.220"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            293..4546
                     /gene="fdeC"
                     /locus_tag="N7A07_004037"
     CDS             293..4546
                     /gene="fdeC"
                     /locus_tag="N7A07_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU2478600.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(4667..5524)
                     /locus_tag="N7A07_004038"
     CDS             complement(4667..5524)
                     /locus_tag="N7A07_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478601.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5773..6642
                     /locus_tag="N7A07_004039"
     CDS             5773..6642
                     /locus_tag="N7A07_004039"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU2478602.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6802..>7226)
                     /gene="rclC"
                     /locus_tag="N7A07_004040"
     CDS             complement(6802..>7226)
                     /gene="rclC"
                     /locus_tag="N7A07_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU2478603.1"
                     /translation="EHPEDYKQYLTHEGEYKPEARAWQSSNNTYGFSNGLGVVEVIIA
                     LLVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRL
                     VLKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
BASE COUNT         1970 a         1615 c         1873 g         1768 t
ORIGIN      
        1 ataatgaatt attcactgtc cgcagagtaa gaaatataac ttaagtatct atttaatgac
       61 ttgcacaaaa agctaaattt tcacccataa ataaaaatat aatcccgcgc ccaaccacct
      121 gatgagtggc tacaggcact ggatatatta ggtggcggtg tactttctta cataaaggta
      181 tttccttttc tgcggaaaag gaaaccggga aatccccggt ttttctgaca agcagacgcc
      241 attatttgtg tctgcctatg ttcgttaatt cgttcatcag gaaattatct caatgtcacg
      301 ttataaaaca ggtcataaac aaccacgatt tcgttattca gttctggccc gctgcgtggc
      361 gtgggcaaat atctctgttc aggttctttt tccactcgct gtcaccttta ccccagtaat
      421 ggcggcacgt gcgcagcatg cggttcagcc acggttgagc atgggaaata ctacggtaac
      481 tgctgataat aacgtggaga aaaatgtcgc gtcgtttgcc gcaaatgccg ggacattttt
      541 aagcagtcag ccagatagcg atgcgacacg taattttatt accggaatgg ccaccgctaa
      601 agctaaccag gaaatacagg agtggctcgg gaaatatggt acagcgcgcg tcaaactgaa
      661 tgtcgataaa gatttctcgc tgaaggattc ttcgctggaa atgctttatc cgatttatga
      721 tacgccgaca aatatgttgt tcactcaggg ggcaatacat cgtacagacg atcgtactca
      781 gtcaaatatt ggttttggct ggcgtcattt ttcaggaaat gactggatgg cgggggtgaa
      841 cacctttatc gaccatgatt tatcccgtag tcatacccgc attggtgttg gtgcggaata
      901 ctggcgcgat tatctgaaac tgagcgccaa tggttatatt cgggcttctg gctggaaaaa
      961 atcgccggat attgaggatt atcaggaacg cccggcgaat ggttgggata tccgcgcaga
     1021 gggctattta cctgcctggc cgcagcttgg cgcaagcctg atgtatgaac agtattatgg
     1081 cgatgaagtc gggctgtttg gtaaagataa gcgccagaaa gacccgcatg ctatttctgc
     1141 cgaggtgacc tatacgccag tgcctcttct gacactgagc gccgggcata agcagggcaa
     1201 gagcggtgag aatgacactc gctttggcct ggaagttaac taccgaattg gcgaaccttt
     1261 ggcgaaacaa ctcgatacgg atagcattcg cgagcgtcgg gtactggcag gcagccgcta
     1321 tgacctggtt gagcgtaata acaacatcgt tcttgagtac cgcaaatctg aagtgatccg
     1381 tattgctctg cctgagcgta ttgaaggtaa gggcggtcag acactttccc tggggcttgt
     1441 ggtcagcaaa gcaactcacg gactgaaaaa tgtgcagtgg gaagcgccgt cattactggc
     1501 tgaaggtggc aaaattaccg gtcagggtag tcagtggcaa gtaacgctcc cggcttatcg
     1561 tccaggcaaa gacaattatt atgcgatttc agcagttgcc tacgataaca aaggcaatac
     1621 ctcaaaacgc gtgcagacag aggtggtcat taccggagct ggtatgagcg ccgatcgcac
     1681 ggcgttaacg cttgacggtc agagccgtat tcaaatgctt gctaacggta atgagcaaaa
     1741 accgctggtg ctgtctctgc gcgacgccga gggccagcca gtcacgggca tgaaagatca
     1801 gatcaagact gaactaactt tcaaaccggc tggaaatatt gtgactcgtt ccctgaaggc
     1861 cactaaatca caggcaaagc caacactggg tgagttcacc gaaactgaag caggggtgta
     1921 tcagtctgtc tttactaccg gaacgcagtc aggtgaggca acgattactg ttagcgttga
     1981 tggcatgagc aaaaccgtca ctgcagaact gcgggccacg atgatggatg tggcaaactc
     2041 caccctgagc gctaacgagc cgtcaggtga cgtggttgct gatggtcagc aagcctatac
     2101 gttgacgttg actgcggtgg actccgaggg taatccggtg acgggagaag ccagccgctt
     2161 gcgatttgtt ccgcaagaca ctaatggtgt aaccgttggt gccatttcgg aaataaaacc
     2221 aggcgtttac agcgccgcgg tttcttcgac ccgtgccgga aacgttgttg tgcgtgcttt
     2281 cagcgagcag tatcagctgg gcacattaca acaaacgctg aagtttgttg ccgggccgct
     2341 tgatgcagca cattcgtcca tcaccctgaa tcctgataaa ccggtggttg gggggacagt
     2401 tacggcaatc tggacggtaa aagatgccta tgacaaccct gtgaccagcc tcacgccgga
     2461 agcgccgtca ttagcgggtg ccgctgctga aggttctacg gcatcgggct ggacaaataa
     2521 tggtgatggg acgtggactg cgcagattac tctcggctct acggcgggtg aattagaagt
     2581 tatgccgaag ctaaatggac agaatgcggc agcaaatgcg gcaaaagtaa ccgtggtggc
     2641 tgatgcgtta tcttcaaacc agtcgaaagt ctctgtcgca gaagatcacg taaaagccgg
     2701 cgaaagcaca accgtgacgc tggtggcgaa agatgcgcat ggcaacgcta tcagtggtct
     2761 tgcgttgtcg gcaagtttga cggggaccgc ctctgaaggg gcgaccgttt ccagttggac
     2821 cgaaaaaggt aacggttcct atgttgctac gttgactaca ggtggaaaga cgggcgagct
     2881 tcgcgtcatg cctctcttca acggccagcc agcagccacc gaagccgcgc agttgacggt
     2941 catcgccgga gagatgtcat cagcgaactc tacgcttgtt gcggacaata aggctccgac
     3001 cgtcaaaacg acgacggaac tcaccttcac cgtgaaggat gcgtacggga acccggtcac
     3061 cgggctgaag ccagatgcac cagtgtttag cggtgccgcc agcacgggga gtgagcgtcc
     3121 ttcagcagga aactggacag agaaaggtaa tggggtctac gtgtcgacct taacgctggg
     3181 atctgccgcg ggtcagttgt ctgtgatgcc gcgagtgaac ggccaaaatg ccgttgctca
     3241 gccactggtg ctgaacgttg caggtgacgc atctaaggct gagattcgtg atatgacagt
     3301 gaaggttaat aaccaactgg ctaatggaca gtctgctaac cagataaccc tgaccgttgt
     3361 ggacacctat ggtaacccgt tgcaggggca ggaagttacg ctgactttac cgcagggtgt
     3421 gaccagcaag acggggaata cagtaacaac taatgcggca ggtaaagcgg acattgagct
     3481 tatgtcaacg gttgcgggag aacacaatat ttccgcttcg gtgaatggtg ctcagaagac
     3541 ggtcacggtg aaattcaacg cggatgccag caccggtcag gcaaacctgc aggtagacgc
     3601 cgctgctcaa aaagtggcaa acggcaaaga tgcctttacg ctgacggcga acgttgagga
     3661 taaaaatggt aaccctgttc cagggagcct ggtgaccttt aatctgcccc ggggtgtcaa
     3721 gccgcttaca ggcgataatg tctgggtgaa agccaacgat gaggggaaag cagagttgca
     3781 ggtggtttca gtgactgccg gaacgtatga gatcacggca tcggcaggga atagccagcc
     3841 ttcgaatacg cagactataa cgtttgtagc cgataaggct accgcaaccg tctccggtat
     3901 tgaggtgatt ggcaactatg cactggcgga cggcaatgcc aaacagacgt ataaagttac
     3961 ggtgactgat gccaataaca acctgttgaa agatagcgaa gtgacgctga ctgccagccc
     4021 ggcaaattta gttctgactc ccaatgggac ggcgaaaact aatgagcaag gacaggctat
     4081 tttcaccgcc acgaccactg tcgcagcgaa atatacactc acggcgaaag tgagtcaggc
     4141 cgacggtcag gaatcgacga aaactgccga atctaaattc gtcgcggatg ataaaaatgc
     4201 agtactcacc gcatcatctg atgtgacttc tctggtggcg gatgggatat cgactgcgaa
     4261 gctggaggtg acactgatgt cggcaaataa ccccgttggg gggaatatgt gggtcgacat
     4321 taagacgcca gaaggggtga cggagaagga ttatcagttc ctgccgtcga aaaatgacca
     4381 tttcgtgagc ggaaaaatca cgcgtacatt tagtaccagc aagcctggtg tctatacgtt
     4441 cacatttaac gccctgacgt atggcgggta cgaaatgaag ccagtgacgg tgaccattac
     4501 cgcggtggat gccgatacgg caaagggcga ggaggcgatg aactaatatt aagaatatgg
     4561 aagtaacatt ctaataaaca ataatggcgt tgacatcttc aacgccatta tttattgaat
     4621 tgcctgacag tagtctcgtt ataagttcat ttagatatag cgacagctaa accggacgaa
     4681 cagggataaa tatttcgcaa acaacatcat tgctatcctt ttctacgaaa tcttcattgt
     4741 aataaaaccg ctcaatatca ctgccgttca ctctggctaa ccccctggcg ggtaattcca
     4801 tcagatagag gtttcgcgcc cattcagggt agtgctccca ctcaccttca tagcggaaag
     4861 aggcatacag tcctcggtta attgtccact ctttcggcat ctctgaatcg gtttgtttgc
     4921 ataccgtacc ggcaaaagtt tcaactgtga gtaaatcgac cgattttgtt gacggttcaa
     4981 acgtggagac gaaatagata tccgtcttaa aattcttcat acatctggct aaatgatgac
     5041 gtcgcagttt gacttcttca tccggtgatt tattcgacac ttcaatatca tatttaaatg
     5101 aattgccaaa aataggtgtc tcaggaaagt tgactaatct acactccgtc ttttgctgtt
     5161 gacgtattaa aaaagaaggg aagatatttg cgagatccca ataatcacgg tggcggtata
     5221 cacggggaga gcagccgaat aactttttaa attcgcggct gaatgactgc tgtgaatcaa
     5281 aatgcaaaga gattgcaata tcaagcatag atttcgcggt gagccggaca agaatggcgg
     5341 cacgacaaag ccttcgtttg cgaatgtatt ctcccaaagg cacatgcatg aaatttcgga
     5401 acagaagctg gatgttgcgt ctgctgtagc cagatttctg tgcgatatct tcgattgaaa
     5461 tagggtgctc aagattgcac tcaatccact ccaggagctg ctgtagaatc ttctgcctga
     5521 tcatcacatt ctccgttaat tctttgtcat atcgcacatt ttcgttatgc cctgggccct
     5581 aagtgtatca aaaacagcaa gggcgaaggc aaaagcactg gtagcgtctg cttcgttgtc
     5641 cccgaaaata gagaggtttc agtcctacat tattaatgaa ttttttgcat aagtgatatc
     5701 aaaatccaca tactaatttg aggttacgtt ttaacgtaga ctcattgttc atgcctaatg
     5761 gagggactga cagtggaatt ttccgtatta agtaacaatc tgaaaatgcc gatgatggga
     5821 tttggtgttt ttcaggttac cgataaaaac gtgtgcaaac agtcagtgtt gaacgctatc
     5881 cgcacggggt atcgactcat cgacactgca gcggtatacg gtaatgaaga tgccgtcggt
     5941 gaagccgttc gtgaagctat atccgaaggt ttatgtaccc gagaagagtt atttataacg
     6001 tctaaattgt gggtgcagga tatgttgaat caggatacgg cagcagcagg tattgaagca
     6061 tcattaaaaa aatcgggact agaatacttc gacctctatt tattgcatca ggctatgcgc
     6121 gattatttca gtgcgtggcg tgcactcgaa gatgcctatg aagagggcaa attaaaagcg
     6181 attggggttt ccaatttcta tcctcatgtt ctggcgaact tttgtgaaac ggtaagagtt
     6241 aaaccgatgg tcaaccaggt cgagttgcat ccctattttg cccaaccaga ggcgctggca
     6301 accatgaagt attataacgt gcagcctgaa gcatgggctc cgttaggtgg tggacgacat
     6361 aaaccctttg aaaataatct gcttcagagt attgcagatg cccatcaaaa atcgatttct
     6421 caagtcattc tgcgttggaa tattcaacgg ggagtggtcg ttattccgaa atcgacacat
     6481 caacagcgta tcgaagaaaa ttttgctatc tgggatttct cactgacaga gaaagaaatg
     6541 gcacaaatta gttcgcttga tttgggttat gttggggagt cggtaaaaca ttttaatcct
     6601 gaatttgttc gtggttgtct tgctgtaaaa atacatgatt gatattaatc atgtatttta
     6661 cctgagacga caagaatctt ttaacagggg agtgatattg atcttcactc tgtcatatct
     6721 ccggtaatat ggcgtcaggc ttcatcatca gaaagggggc ctgacgcctg aaaaagtgaa
     6781 caacagacag tgttcggatt atcaatattc agtttttaac gttgaactgg attcattact
     6841 gccttgttta agaatttccc gcgccgaatc tgccattatc atgacagcac ctgccagcat
     6901 cagagtatct ttcaatacca gacgaccagc accggataaa taagggaaac catgatgagc
     6961 gtcacccaat gcgggtaccc atgcctccgg ggtggtgatt aaaaatgaga gtgttaccag
     7021 cggtgtggta aatgccatca gccctcccaa taaacctaac cagcgattga caggattagc
     7081 caaaaccagc agagcaataa tcacctccac gacgccaaga ccgttggaaa aaccataggt
     7141 attattggac gattgccatg cccttgcttc tggtttgtat tcgccttcgt gagtcagata
     7201 ctgtttatag tcttccgggt gttcat
//
LOCUS       DBFIFS010000221         7188 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.221, whole genome shotgun sequence.
ACCESSION   DBFIFS010000221 DBFIFS010000000
VERSION     DBFIFS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.221"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(371..643)
                     /locus_tag="N7A07_004041"
     CDS             complement(371..643)
                     /locus_tag="N7A07_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU2478604.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(649..1200)
                     /locus_tag="N7A07_004042"
     CDS             complement(649..1200)
                     /locus_tag="N7A07_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU2478605.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1197..1949)
                     /locus_tag="N7A07_004043"
     CDS             complement(1197..1949)
                     /locus_tag="N7A07_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HGU2478606.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            2883..3143
                     /locus_tag="N7A07_004044"
     CDS             2883..3143
                     /locus_tag="N7A07_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2478607.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3140..3697
                     /locus_tag="N7A07_004045"
     CDS             3140..3697
                     /locus_tag="N7A07_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2478608.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            3694..3915
                     /locus_tag="N7A07_004046"
     CDS             3694..3915
                     /locus_tag="N7A07_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478609.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            3915..4238
                     /locus_tag="N7A07_004047"
     CDS             3915..4238
                     /locus_tag="N7A07_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HGU2478610.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4252..6585
                     /locus_tag="N7A07_004048"
     CDS             4252..6585
                     /locus_tag="N7A07_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU2478611.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(6718..>7188)
                     /locus_tag="N7A07_004049"
     CDS             complement(6718..>7188)
                     /locus_tag="N7A07_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478612.1"
                     /translation="ANGHVTSTGFSRNLMDAINISLAEHKIASLHNEVMSIEEYDRDS
                     LQEKGNTARRYLEYILMLVNIRIMHLNNVQYQEQMLGSLVSVIEALDYEPLMKNDVEI
                     TKDILNACSHHGGVRIEKKDVIFSLEVIENLIKAIKKTDINKLQLDGMFKSIQK"
BASE COUNT         1714 a         1715 c         1781 g         1978 t
ORIGIN      
        1 tatatttcgt ttcgagcgct cagttcattt ctaaataatt tgcaaaatta ttttgggtac
       61 tttcgcctct acacttcctt cgggtggttc cacaataatg gatctcaccg gcgtttgata
      121 ttttataatc taacccctta gcgcgcgcag ccccccacgc ctgctcgctt cgcttaacag
      181 actggttttc atgcattccg caaatcgtct cagaagccac cacacaaggg ctttcgcgtc
      241 aaaaatggtg catgagaatc atgcgttttc atgcgctata gacatgcact catgcgctct
      301 caggccagcc agggaaaagg cgtaaaaaat cccagtactg gaccgggatt acgtgggctt
      361 tttttgctaa tcagacagga atttgctgac ggcttgacag ctttgacgcg gagccatagc
      421 gattgagagt ttgctgttgt ttttccggca tttgtgcttt ctctgattcc tgttcattgc
      481 gccggggctt catgattatc gtatccacac tttccagtgc ggtaaatgtg taggaacatt
      541 caagattctg gcactgatac catgagcgtt taaccgatgg tgcttcatag gcggaggttc
      601 tggcgtgtgc cgtcgtaccg cattcgggac atttaagtgc cattaatatt actcctgcat
      661 gccgttaatg taatccagac gttgctgaaa gcgcagttgt tgtgcagggg tgtaagagct
      721 ctggcaatca cgaaccatca tggggtcagg catcacaccg gattcatcca gaatcatacg
      781 ataatccccg gcaatttcag gcggtgttat tgccagctgg cgaaccagag cattgcgtaa
      841 tatacgggac gcaacgtcaa cccctccgcg ccccttcagg aacggagcca gtgcagatgt
      901 cagggctgct ccattcgcct gcataaaatt gctgagagcg tcttctgtgc aggcctccat
      961 cagttgatgc tgcgagtaaa tgcattcgcg cgcagcacag ttaatttgcc atttcagcac
     1021 atccagatgt tcccgctgta attcgagctg tcgagcataa tctgctggtt tttccatggc
     1081 agtgaggagt gattctatct gttgttcaac ggcttttcgg ctctcactat gtccagccca
     1141 cagaatacgg gcatttctaa aactgataag cgcttggtct ggagtgactc gggccatcat
     1201 actgctttct ccttctcagc acgttcctga cgaaccttca ttttatgcat agctgctgga
     1261 gagagtttat tactgaaatc cagacgggcc gcataatccg gggcaactcc cgccagttta
     1321 aacacggggt cctgttcggg catcgcatcg ttagtcagag ccggtctggt gatatgttta
     1381 tgaataaaat ctttcagcag tgtgtccggg tcattaactg aatgcaccac accgacaact
     1441 gcgctggctt ccctgcccat tgttgatttg agcaggctta gtgtctggat tagtgctttt
     1501 ccatgcgact gcataaaatc ttcccagatt tgttttgcgc gaatacccac aagggtgtcg
     1561 tgcgcgtgga tatactttcc ggctaattcc gcagcctcct gcggcaaaag tgcattttcg
     1621 ctttcctgag ccgccagcag ctcatcaaaa tcctcaagcg tttctcgtcc cagcgcaatt
     1681 tccgtgcgca gttttttcat ttcctttgag actacgccct gactttcacg aaatagcgtg
     1741 cgccactcct cattcagggc gttcgtcgtg gcttccattt cagcgcggcg ctggcggatt
     1801 gtcgcaatat tatccgctgc cgctttttgc tgacgacgtg cttcaagcca tgcagatttg
     1861 gtggcattca ctttctcaag cgcctgttgc gtcgaaacag ggatagaaat ttcaacgatc
     1921 gggttattgt tctgttctgg gattgtcatg tcagcaccta gtggtttgtc gtggtgtcaa
     1981 ttgtgctgtg atgcatacaa agctgccatt gaacgccatt gtgcgagcga caagacaaag
     2041 actgaaccgc cccggacctc ttttggctag ccagaaaagg cctcgtttaa ctaaggctgt
     2101 ttcagactta ccttaactat tcactactat tcacttaggt aaaaaaaata ggtaatacag
     2161 aaggttataa ggtgaagggt tggcatggca gtattcatcg actgttcata actgttcaca
     2221 tgggaataaa attatttatg gtattttttc ataatgggtg gtttttttat tcctcgtagc
     2281 tatttgtttt ttgctcctca ccactattca cccttattca ggagtattca acaggcacat
     2341 aaatagactg catgtttttg ccggggaata acattttcgc ctctggccat ggtgttgcat
     2401 ttcatactga tttttataaa actagatctg tccggttctt tatggattca ttcggatata
     2461 ttttacgatt taaggtgatg ttatttaata tcaaggtttt tatgcaggcc caatgtttat
     2521 aagttggatg cgattcaaaa atatgcccgg aaattggggc ctgcaatgca aggcctcgtg
     2581 tagaagttgt actgccttgc tgtattggcg gtggggttag cactatcagg tgcaatgacc
     2641 cataggatag taaaccctgg gtgaatggta gttcctatta atgctcctgt atttatagtc
     2701 cgcaggcatg gtatttatta tgtcatttta tatggtttaa tcgggcttta aattccataa
     2761 aatcaatatg tgacgctaaa taaaaaatgc gtgcgcaggt ggtttatata cacataataa
     2821 ggaactacct gaacccggat aaattcatcc ggactgttgt ggaaattaaa gagggtagat
     2881 aaatgcgtaa tatttctgtg tctgcgccag ctcctgctgc gccattattt cctgtatcga
     2941 accatcatga gcgattttta cgcctgcctg aggtgatgca cttatgcggg ttgtcccgtt
     3001 cgaccgttta cgacctgatc agccgcgatg cgtttccgaa gcagatcccg cttggcggta
     3061 aaaatgtcgc ctgggcacag tctgaggtca gcgcatggat ggcggaccgc atcagcgccc
     3121 gtgggcgggg atgtgatgca tgatgatacc tgcatacatt aaataccttt attctggctt
     3181 gcttactgtc gtcatttcca ggtatagttt ttccgctgtc gcaaaatcgg cagccggaat
     3241 tggcgttccg tataacttat tggcgacaat tgacgcccct tgcgtctttt tttacgtcgt
     3301 agctcaggca caaccatttt cagggctgtg gtgtttatgc ttacaccatg gttctatcga
     3361 gataatggta gtccgggcgg ggcagccttc gggctggccg gtttccaata aggccggtta
     3421 cgccaacccc gttcgggctg ccaccagtga aattggcgtt tccggtggta gcaataaccg
     3481 ctacttattg gaggctgcca tcatggctac aatcctcacc ccgtcacacc cgcaatatgt
     3541 ctttgtgttt gccgcaatcc gccgtgctga cactcacccc cgcatctgta tgcttcgcac
     3601 tgtctcctgc gatgagcgtt ccgcgcgccg tttgctagtc cgagattatg tactctccct
     3661 ttctgcacgg ctgcctgccg gggaggtgac gctatgaacc agtttgagat ttcctacgac
     3721 gatgttgtaa ggctgaaaca tttacgcaat gtgggtgagt atgtaactgg catggcagcc
     3781 ctgcaggact gttatgaaaa gccagcaggt gcccaatgcg aacaactggt ttccctcatt
     3841 tatctgatga cagagcaact ggatggagtg gtgcaacgct gccatgacga cctgatgaat
     3901 gcggaggtgg cctgatgata tgctgtgaat ctcttctggc acttcgtgcc gctctgtatc
     3961 gccgtgccgt tgcctgtgcc tggctggctc taagcaatca tcaggaacgt tattccggcc
     4021 tgacgctcgc tgaacttgaa gatgccatag cccgcgagct ggaagggttc tatctgcgcc
     4081 agcatgggca gcaaagaggg ctggaaattg cctgcgcgtt gctttcggat ctgatggagt
     4141 cggggccgct taaagcctgt ccggttctct cactgctcgg aatgacggtc atggatgaac
     4201 tttgttcccg tcacctcaac aaaccagcgc tgcactaagg agggccgcac aatgtcagga
     4261 atgaaagtta gccaggctga gaaagcagct cggggtcact ggtcaagaat tttacctgcg
     4321 ctgggcgtaa atgtactgaa aaatcggcac cagccctgcc cggtctgtgc cgggaaagac
     4381 cgctttcgat ttgatgacca ggaagggcgg ggaacgtggt tctgtaacca gtgcggggca
     4441 ggtgatggcc tggcgcttgt aagtaaagta ctggatgtag gcattagtga agcggcagac
     4501 agaataaacg gcattatcgg aaacctgctg ccagtatctc agggaatgct tgaatctggt
     4561 tctcctgaaa aagaggacgg gagaaaagct gcagcagtgc tggctgcccg tttgtttgat
     4621 aagtcccgcc agaccactgg caatgcctat ctgacgagta aagggtttcc tgcactgcct
     4681 tgccgggaat taaccgctat gcataaagtc ggtggtgtgg catttcgcgc gggagatctt
     4741 gtcgttccat tgtatgcaga tggagagctg gtaaatctgc agttaatcaa cgctaatggg
     4801 ggcaaatgct tccttaaagg cggtcaggtt aagaatgcct tttacctggt tgaaggtact
     4861 gccaaagcag ccaaacggct ctggatagcg gaaggatatg ccaccgcact tactatcaac
     4921 tatctgactg gcgatgctgt catggtggcc ttttcgtccg tcaatttcct ttccctggcg
     4981 agcattgcct gcagtgagta cccaacgcac cagataatta ttgctgctga ccgcgatctc
     5041 aacggtgcgg ggcaaacaag gggcgcagct gttaccgggg cctgcaattg cacaatggcg
     5101 ctcccgcctg tgtttggtga ctggaacgat gcattcacgc aaaacggcga agaagccacc
     5161 cggcatgcaa ttcatgaagt aataaaacca gctgttgcca gccccttcga cacaatgagc
     5221 gaagctgaat ttaccgcgct gagcgtcagc gaaaaagcgc agagggtagt ggatcactat
     5281 aaaaattcac tggcagtaga cccgaacggg cagctccttt cacgctatga ggcgggggcc
     5341 tggaaagtta tctattacgc cgattttgcc cgtgatgtcg ctgcgctgtt tcagcgcctc
     5401 gacgcacctt tttcatccgc gaaaattgcg tctctcgtgg aaaccctcaa actgatcgtt
     5461 ccgcaacagc agaatccggc gcggcaactt atcggatttc gcaacggtgt gctcgatacc
     5521 cggacaggat tgttcagccc gcacgataag aagcactggt tacgtacgct gtgcgaggtg
     5581 gattacacgc agcccgttga cggtgagtca ctggaaaccc atgccccggc attctggcgc
     5641 tggctggatc gtgccgcagg ttttaatcct gaaaaacggg acattattct ggctgcattg
     5701 tttatggtgc tggctaaccg ttatgactgg cagctgtttc tggaggtcac tggccctggc
     5761 ggaagtggaa agagtattct tgctgaaata gcaaccatgc tggcgggtga agataacgcg
     5821 acctcggcaa ccattgaaat gcttgagtcg ccaagagaac gagctgcgtt aataggtttt
     5881 tcactgattc gacttcccga ccaggaaaag tggagcggtg acggggccgg actaaaagcc
     5941 atcactggcg gcgatgcggt atccgttgat cccaaatatc agaacgccta ttcaacccac
     6001 atcccggcgg tcatcctggc tgtgaacaat aatccgatgc gcttcactga tcgtagtggt
     6061 ggagtttcac gccgaagggt gatcctgcat ttccccgacc agatagcccc ggaggaacgc
     6121 gatacccagc tcaaagagaa aattgccagt gagctagcgg tgattgttcg ccagcttatg
     6181 cagcgtttca gcgacccaat gagtgccagg acattgcttc agtcacagca gaactccgat
     6241 gaagcgctca ccatcaaacg tgatgctgat tcagcttttg atttttgcgg ctaccttgag
     6301 gtcctacctg acaccacggg catgtttatg gggaacgcta atattgttcc acgtcagcct
     6361 cgaacttacc tctaccatgc ctatctggtc tacatggagg ctaacggcta taaaaatacg
     6421 ctcagtctga ccatgtttgg caaggggcta ccgttaatgc tcaaggaata tgggctgcag
     6481 tatgagaaac gacggaccaa tcaaggaatg cagactaatc tggccctaag agaggaaagc
     6541 aatgctgact ggttgccaaa atgcgatgag tttgcagcga aataactgac ctaagaccgg
     6601 ctgtgccggt cttaaatccg ctacaggaaa ttatctcaaa atatatatcg ataaaattta
     6661 aaagttatat gctaaggcgt cagcggaaaa tgcatctaat tcattgttag atgatgttta
     6721 tttttgaatt gatttaaaca tcccatcgag ttgtaattta tttatgtcgg ttttctttat
     6781 ggctttaatt aagttttcaa taacttccaa agaaaaaatt acatctttct tttcaattct
     6841 aacccccccg tggtggctgc aagcattaag gatgtccttt gtaatttcaa catcattttt
     6901 cattaatggt tcatagtcca gagcctcaat aacagaaact aaactaccta gcatttgctc
     6961 ttggtattga acgttgttta aatgcattat acgaatatta acaagcatga gaatatattc
     7021 taaatatctc ctggcagtat ttcctttctc ttgtaaagaa tcacgatcat attcctcaat
     7081 actcatgact tcattatgta aagaggctat tttgtgctct gctaatgaaa tatttatcgc
     7141 atccatgagg tttcgtgaga agcctgtaga tgtaacatga ccatttgc
//
LOCUS       DBFIFS010000222         7099 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.222, whole genome shotgun sequence.
ACCESSION   DBFIFS010000222 DBFIFS010000000
VERSION     DBFIFS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.222"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(199..1335)
                     /locus_tag="N7A07_004050"
     CDS             complement(199..1335)
                     /locus_tag="N7A07_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2478613.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1338..1700)
                     /locus_tag="N7A07_004051"
     CDS             complement(1338..1700)
                     /locus_tag="N7A07_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU2478614.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2237..4150
                     /gene="mtlA"
                     /locus_tag="N7A07_004052"
     CDS             2237..4150
                     /gene="mtlA"
                     /locus_tag="N7A07_004052"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU2478615.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4245..5393
                     /gene="mtlD"
                     /locus_tag="N7A07_004053"
     CDS             4245..5393
                     /gene="mtlD"
                     /locus_tag="N7A07_004053"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU2478616.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5393..5980
                     /gene="mtlR"
                     /locus_tag="N7A07_004054"
     CDS             5393..5980
                     /gene="mtlR"
                     /locus_tag="N7A07_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU2478617.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5992..6201)
                     /gene="yibT"
                     /locus_tag="N7A07_004055"
     CDS             complement(5992..6201)
                     /gene="yibT"
                     /locus_tag="N7A07_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU2478618.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6486..6848
                     /locus_tag="N7A07_004056"
     CDS             6486..6848
                     /locus_tag="N7A07_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU2478619.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1815 a         1775 c         1793 g         1716 t
ORIGIN      
        1 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
       61 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      121 catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      181 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      241 tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg
      301 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      361 cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct gcgcctgata
      421 agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg
      481 tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg
      541 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag
      601 cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      661 cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      721 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      781 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      841 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca agtaacgttg
      901 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
      961 agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1021 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1081 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1141 agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1201 taaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1261 atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1321 aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1381 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg aggcgatttc cgggtcagtc
     1441 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1501 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1561 gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag ataagggatg
     1621 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1681 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gtttttgggc
     1741 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1801 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1861 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     1921 attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta ctcacattta
     1981 aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg ttttgtgatg
     2041 aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc acacaaaagg
     2101 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2161 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2221 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2281 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2341 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2401 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2461 acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc
     2521 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2581 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2641 aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc cgattgttga
     2701 aaccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2761 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2821 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat caatcttctt
     2881 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     2941 tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat
     3001 ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct
     3061 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3121 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa
     3181 catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa
     3241 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3301 gaaagctgag tctaaaggtg catctccgct gtctgcgggc gatgtgacta acgacctgag
     3361 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg cgatgggcgc
     3421 aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca cagatttcgg tcactaacag
     3481 cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3541 acgcgctatg cgccaggttc ctcaggcaca gcatatttcg ctaaccaact tcctcgacag
     3601 cggcctgtac accagcctga ctgaacgtct ggttgctgcc cagcgacata ctgaaaacga
     3661 agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3721 actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag aagcgattcg
     3781 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggagtacg ttcaggcgat
     3841 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     3901 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     3961 aggcgtgcgc ttcggtgaag aagaagatga cattgctcgt ctggtgattg gtattgctgc
     4021 ccgtaacaac gagcacattc aggttatcac cagcctgacc aacgcactgg atgatgagtc
     4081 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg
     4141 tcgtaagtaa tccaatccta ccctctcctc acggagaggg tttgggtgag ggaaaagcct
     4201 caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt
     4261 ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac gcgggtatcc
     4321 aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct
     4381 atcaggttca tgtggtcggt gaaaccgagc aggtagatac cgtttccggc gtcgatgctg
     4441 tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggtcgattta gtcactaccg
     4501 ccgttggccc ggttgtgctg gaacgtattg ctccggctat cgccaaaggg ctggtgaaac
     4561 gtaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac atggtacgcg
     4621 gtaccacgca gctgaaaggt catgtgatga acgccctacc agaagacgcc aaagcgtggg
     4681 tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg ccttcggctt
     4741 cggcaactaa cgatccgctg gaagtgacgg tagaaacttt cagcgaatgg attgtcgata
     4801 aaacgcagtt caaaggcgca ctgccgaaca tcccaggcat ggagttaacc gacaacctga
     4861 tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata accgcgtacc
     4921 tcggaaaact ggccggtcat cagaccattc gtgacgctat tctcgacgag aaaatccgcg
     4981 cggtggtaaa aggtgcgatg gaagaaagtg gtgcggtact gatcaagcgc tacggctttg
     5041 acgcagacaa acatgcggcg tacatccaga aaatcctcgg tcgttttgag aacccgtatc
     5101 tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt gctggcgacc
     5161 gtctgatcaa gccattgctc ggtacgctgg aatacagcct tccgcacaag aatctgattc
     5221 aggggattgc tggtgcaatg cacttccgca gtgaagacga tccgcaggct caggaactgg
     5281 cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc gatcttgatg
     5341 ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa taatggtgga
     5401 ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga
     5461 acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg
     5521 aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca
     5581 ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga
     5641 cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa
     5701 ccgccaggaa tacgaagatg cggagctgct gatggcattg cgtgaagaac taaatcacga
     5761 cggcaacgag tacgccttta ccgacgacga gatccttgga ccctttgggg aactgcactg
     5821 cgtggcggcg ttaccaccgc cgccacagtt tgaaccagca gactccagtt tgtatgcaat
     5881 gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac tgactgagct
     5941 gatttccaga atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc
     6001 ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta gatataacgg
     6061 cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt ttttcagata
     6121 ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa gaagcggaac
     6181 gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg
     6241 ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt gctgaatcct
     6301 caataatcat ccgctgctgt ttattatctt ttttctatag atttcttaat taatcaataa
     6361 attgcgtagt ttttaagcat gttaatgatt acgttacaga actattgcaa tgtcatcagt
     6421 tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt atcagaagcc
     6481 atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgt ctggacgcca
     6541 tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg
     6601 aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgtgaagtt catagccaaa
     6661 aactgagtaa agaagcacag aagctgatga agatgccgtt tcagcgcgca attaccaaaa
     6721 aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg gttgtgcacc
     6781 caatgaccgc gctgggccgt gaaatgggcc tgcaggagat gactggattc tctaagactg
     6841 cgttttaagc gcctcagacg actggttcga tcatatcaac ccgcatctgg cttgccttag
     6901 ctggtcaatg cggggactct cctgtctact cgcctaatta ccaaacaatt cactcccttt
     6961 acgtcgattt accgctggtt gatattctga aaatgcattt ttaccgaaat agccacctat
     7021 aaacaataaa aatatataga agatatcatt tattaattaa caagcagtta cgtctctttt
     7081 tttcggattt aattgtgaa
//
LOCUS       DBFIFS010000223         7089 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.223, whole genome shotgun sequence.
ACCESSION   DBFIFS010000223 DBFIFS010000000
VERSION     DBFIFS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.223"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..474)
                     /locus_tag="N7A07_004057"
     CDS             complement(<1..474)
                     /locus_tag="N7A07_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU2478620.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGT"
     gene            complement(487..2268)
                     /gene="gcl"
                     /locus_tag="N7A07_004058"
     CDS             complement(487..2268)
                     /gene="gcl"
                     /locus_tag="N7A07_004058"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU2478621.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2358..3173)
                     /gene="allR"
                     /locus_tag="N7A07_004059"
     CDS             complement(2358..3173)
                     /gene="allR"
                     /locus_tag="N7A07_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU2478622.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3251..3733)
                     /gene="allA"
                     /locus_tag="N7A07_004060"
     CDS             complement(3251..3733)
                     /gene="allA"
                     /locus_tag="N7A07_004060"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU2478623.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3963..4889
                     /gene="allS"
                     /locus_tag="N7A07_004061"
     CDS             3963..4889
                     /gene="allS"
                     /locus_tag="N7A07_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU2478624.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4958..6052
                     /gene="mnmH"
                     /locus_tag="N7A07_004062"
     CDS             4958..6052
                     /gene="mnmH"
                     /locus_tag="N7A07_004062"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU2478625.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            6168..6533
                     /gene="ylbG"
                     /locus_tag="N7A07_004063"
                     /pseudo
     CDS             6168..6533
                     /gene="ylbG"
                     /locus_tag="N7A07_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1804 a         1850 c         1729 g         1706 t
ORIGIN      
        1 agttccggtg agatggaaac caggaatatc aaaatggtta ataggttcaa tcagtaataa
       61 aatatcttct ttcatcagca tattcgcggc ataacgcagg ttttctacaa gcgttgcgtg
      121 aatctgttca ctgctgaaac cagccggcgt tttaccgacc agacagttaa tatttttatt
      181 acccagcgca cgggcataac gaatcgctgc tgctacgcca tcccgaaact cttcttcacg
      241 gccaggaata caggcaatac cgcgctcccc cgccgcccag tcacccgccg gtaaattgtg
      301 cagcgtatgt tcgagtttat tactcgccag cacctgtttt aattcttcaa tgtcgtagtc
      361 ataaggaaac ataaattcaa cgccgcgaaa accacactgc gcagctttct caaaacgggc
      421 gagaaaatca tattcaccaa ataacatcga taaattagca gagaaacgta acattattta
      481 tctcccttat tcatagtgca tgaagcaggt ttcagtcggt gcatccgctg cgttatcggc
      541 gatatcttca aattccatga cgttatccag ttcgctgccc atcgaaatat ttgtcacacg
      601 ctcgagaata acttccacga ctaccggtac ccgatattgc gccattaagg ctttcgcctg
      661 ttcaaaggcc ggtgcaatat cttccggttt gaagacgcga atagctttac aacctaaacc
      721 ttccgctact tttacgtggt cgacgccgta accgttcact tcgctggagt tgatattctc
      781 gaaagcgagt tgcacgcagt agtccatgtc aaaggcgcgt tgtgactgac gaatcaggcc
      841 gagataggcg ttattcacca gcacatggat gtacggaatg ttgaactgcg cgccaacggc
      901 taactcttca atcaggaact ggaagtcgaa gtcaccggaa atcgccacca cattgcgttt
      961 cggatcagcg gcacaaaccc ccagcgccgc cggaatcgtc cagcctaacg gaccagcctg
     1021 accacagttg atccagtggc ggtctttaaa gacatgcagc atttgtgccg cagcgatttg
     1081 tgacaggcca atggtggtga cataacaaac atcgcgacca aaggctttgt tcatctcttc
     1141 atacacgcgc tgcggtttca ccggcacgtt gtcgaaatgg gttttgcgca gcaaagtgcg
     1201 tttgcgctgc tggcaatcgg cgacccattc tttacgacac ggcagacgac ccgctttttg
     1261 catctcctgc gccacttcaa ccagcagtgt cagcgccgct ttagcatcag agacaatgcc
     1321 gagatccgga cacagcacgc ggccaatttg cgtcggctca atatcgatat gaacgatttt
     1381 gcgcccttcg gtgtatttct ctaccgaacc ggtatgacgg ttagcaaaac ggttaccgat
     1441 accaaacacc atgtcggacg ccagcaacgt tgcgttaccg taacgatgcg cggtttgcag
     1501 accgaccatc ccggccatca gttcatgatc gtccgggata cagccccagc ccatcagcgt
     1561 tgggatcacc ggaacgctgg tcagttcagc aaactgttgt aacagtgcag ctgcgtcagc
     1621 attgattacc ccgcccccgg caacaatcac cggacgttcg gcctggatta acatttctac
     1681 agctttttcg atctgcatac ggctggcagc aggtttgtag accggcagcg gttcgtacat
     1741 gtcaggatca aactcgattt ccgcaacctg aacgtcgaac ggtaaatcca ccagtaccgg
     1801 acccggacga ccagaacgca tcaggtgaaa tgcctgttgc agcacgcgag gcaccagcgc
     1861 cgcttcacga actgtaaccg ccattttgct gaccggttta gcaattgctt caatatctac
     1921 ggcctgaaaa tcttctttat gcagacgggc gcgcggtgcc tggccggtaa tgcacagaat
     1981 aggaatggaa tcagcagaag cggaatagag cgcggtgatc atgtccgtgc ccgcaggacc
     2041 ggaagtcccc agacatacgc cgatattccc tgccgttgcg cgggtataac cttccgccat
     2101 gtgcgaagca ccttccacat gacgcgccag aatgtgacga ataccgccgt gcttacgcat
     2161 cgctgagtag aacggattga ttgcagctcc cggaacgccg aaggcggtgg tgataccttc
     2221 tttctccagc acatacattg ccgcgtcaac ggctctcatt tttgccattt tattcctacc
     2281 tctattaatt ggaaaaattt tccaactttc aaattttgtc aaaaaaaccc gctccgcaga
     2341 gcgggatgcg acagacacta tggatgtgct ttcagtccca acgccgtgct gatatcgcgg
     2401 gcggtgtctc tgaccagctc accctgactg acaaaacgat cttctgtcag tcttgatgaa
     2461 ggcccggaga tggagatagc ggcaacaacg ctacctacat catcgtaaat tgctgaagct
     2521 atgcaattca gacctacaac atgctcttct ttatctacgg tatagcccag ttcacgcgct
     2581 tgttccaggt ccttcagcag ggtgggcata tccacaagcg tagttggcgt aaactgctgc
     2641 aaaccggttt gcagaatgat gctcatcaac tcctcttccg ccagcggata aagcagcgct
     2701 ttgcccgcac cggaagcatg cagtggcaga cgactgccca gtggcgcgca catcctgacc
     2761 atcgatttac actctaactg accaattaat accgcttcat tgccgttacg gatcgcgaca
     2821 ttgaccgttt cgccggaaag taacatcagg cggcgcataa acggcccggc gacggagagg
     2881 acatcgcggt tatggatgta cgccgcaccg acgttaaaga cacctaatcc tatatgccac
     2941 cagcctaact gactgtcctg atagacaaaa tccgctgcct gtaaaacctt cagcaagcga
     3001 aaggtcgtgg agagcggcaa atccagattg agagaaatat cgctaaccga cgaacttccc
     3061 ccactttttt ccagatattg cagaatcgca attccccgct ctaatgcctg tgcgcccttc
     3121 tgtgccacag gctccgcctg tcctggcctg ccgcgccgtc taacttccgt catacctacg
     3181 ttaactccga cctacaagtg agcataaaca atgaacaatt agtttatctt atgcaagtcg
     3241 gttgcaggcg tcacgcaaaa cagagttcct gttcaggaat actttcaaca tcacagttgt
     3301 cactgccgcc gcgatcgatg gtcagaaaat cggtgacgcg ctgccaggcg aaaagtgggt
     3361 gatgccagac gttacgatgg taattcactc cctgttcgcc gttggtaata aacgcccgca
     3421 gggttgacag gtctggtttg tcgtcaccta acgctacgac caccacaaac acctcacctt
     3481 tcatcgggat aaaggcctga gtacccagcg gatgacgttc gagttcgtga atggtcagcg
     3541 gcagattcgc cggttgcgct cggttaatgc tgataagcgt acggtcttgc tcaagaatct
     3601 caaccagcgc caaatcgtgg taacgctcca ccaggccatt gttaatatgg aaaaaatccc
     3661 gttgctgcgt ttcgattacg tcgccataag cactaaaggc ttcctgactt aacggtaata
     3721 cctgaagttt catctttttg tccttgtaac ataattccgt tcaaccagcg ccgaccggtc
     3781 agcttggttt caataacagg caatctaaaa ccttaagaga acaacatcaa cacaaaactt
     3841 ggaatatttt tccaaaacta gatttagtcc acaaattgaa cagggttatc atgtaaaaat
     3901 actgataaat cagaggataa gaaaatcacg caacttgcgg atttaaaagg ggatacctga
     3961 agatgttcga tccagaaacc ttgcggactt tcattgcggt tgctgaaaca ggaagttttt
     4021 caaaagcggc agaacgatta tgtaaaacca cggcgacgat cagttatcgc attaaacttc
     4081 tggaagagaa taccggagtg gcgctgtttt ttcgcacgac tcgtagcgta acgttgacag
     4141 cggctggcga gcatctactt tcccaggcca gagactggct gagctggctg gaaagtatgc
     4201 ccagcgagct gcaacaggtg aatgatggcg tggaacgcca ggtgaatatt gtcatcaaca
     4261 acctgctcta caacccccag gccgtcgccc agttgctggc gtggctgaat gaacgttacc
     4321 cctttaccca gtttcacatc tcccgacaaa tctatatggg cgtctgggac tcgctattgt
     4381 acgaaggttt ttcactggct atcggcgtca cgggaaccga agcgctggca aataccttta
     4441 gtcttgatcc cttaggatcg gtgcaatggc gatttgtcat ggcggcggat catccgctgg
     4501 cgaacgttga agagccgcta acagaagcgc agttgcggcg ctttccggcg gtcaatattg
     4561 aagacagcgc ccgcacctta accaaacgcg tcgcctggcg attgccaggg caaaaagaga
     4621 ttattgttcc tgatatggaa acgaaaatcg ccgcccatct ggcgggcgtt ggcattggtt
     4681 ttttgccaaa atcgctttgc cagtcaatga tcgataatca acaactggtc agccgggtaa
     4741 tcccaacgat gcgccctcct tcgccattga gtctggcatg gcgcaaattt ggcagcggca
     4801 aagcggtaga agatattgtg accttgttta cccagcgcag gccggaaatc agcggatttt
     4861 tagaaatttt cggcaaccca cgcagttaaa aatcaagttt ccttgcgcgt tggcgcgtaa
     4921 aatagcgcgc aagctgattt gaggttcact tttaactatg caagagagac acacggaaca
     4981 ggactatcgt gccctgctga ttgctgatac gcccattatt gatgttcgcg cccctatcga
     5041 gtttgagcaa ggcgcaatgc ccgccgctat caatctgccg ttaatgaata acgatgaacg
     5101 cgccgccgtt ggcacctgct ataaacagca aggctcagac gcagcgctgg cgctgggaca
     5161 taaactggtt gcgggtgaaa ttcgtcagca gcgcatggac gcctggcggg cagcctgcct
     5221 gcaaaatccg caaggtattc tctgctgcgc ccgtggcggt cagcgctcac atattgtgca
     5281 aagctggttg catgcagcgg ggattgatta tccgctggtg gaaggcggtt ataaggcact
     5341 gcgccagacc gcgattcagg cgactattga actggcacaa aaaccgatag tgctgattgg
     5401 cggttgtacc ggcagcggta aaacgctgtt agtgcagcaa cagccgaacg gtgttgatct
     5461 ggaagggttg gcgcgtcatc gcggttcggc gtttggtcgc acgttacaac cacaacttag
     5521 ccaggcgagt tttgaaaacc tgctggctgc cgaaatgcta aaaaccgacg cccgtcagaa
     5581 tttgcgcctg tgggtgctgg aagacgaaag ccggatgatc ggttcgaatc acctgccgga
     5641 atgcctgcgc gagcgaatga ctcaggcggc gattgcggtg gtagaagatc cgtttgagat
     5701 ccgtcttgag cgcctgaacg aagagtattt cttgcgtatg catcatgatt ttacccacgc
     5761 gtacggcgac gaacagggct ggcaggaata ttgcgaatac ctgcatcacg gactttcggc
     5821 tattaagcgt cggctggggc tacagcgata taacgaactg gctgcaaggc tggatgcagc
     5881 actgacaacg caactcacca ccggcagcac cgacggtcat ctggcctggc tggtgccgtt
     5941 acttgaagaa tattacgacc cgatgtatcg ctatcagctt gagaaaaaag cggaaaaagt
     6001 tgtctttcgc ggtgagtggg cggaagtggc ggaatgggtt aaggcgcagt aatgagggac
     6061 gtatttgtat tagtcgcgac ttgatctgac acatggcctt gaaaggttga gagttaccgg
     6121 ttttgatatg agtgtcgaat ccttatccaa aacaagaggt aactctcatg cttcatactg
     6181 ccaatcccgt catcaaacac aaagccggtt tgctcaatct ggctgaagaa ctcagcaacg
     6241 tatcaaaagc ctgtaaaatc atgggcgttt cgcgtgatac gttttatcgt taccgcgaac
     6301 tggtcgctga aggcggtgtg gatgcgcaga ttaatcgtag tcgccgcgct cctaacctta
     6361 agaaccgtac cgatgaggca actgaacagg ctgttgttga ttacgccgtc gctttcccga
     6421 cacatggtca gcaccgggcc agcaacgaac tgcgtaaaca gggggttttt atctccgaca
     6481 gtggtgtccg ttctgtctgg ttactccata accttgagaa tctcaaaaga cgatactgaa
     6541 caaattttat caggtgacgt tccgtaaaaa gttgtatgga gttctcgatg cagtcggcgc
     6601 gactatcagt acccccgctg aaccctggac tatcgggccg ccgtactggg gcatgtcacc
     6661 ctggcgtgcc gctggttttc cgctaatggt ttatttcctc tttctttccc ggcaagaatt
     6721 tattttaatc cggaaggatt atttattttc agatagggac tcacattaaa cacgtaacat
     6781 tttaattaat gcatggctga atatgtatta cacaatgtca gatatcgtaa aagaccagtg
     6841 caattctatg taaactctct gactgttcat tttatttgtt gtttcagggt cggaactcac
     6901 cctcaataaa caactgaata cgagtagata ttcatcgtct gagctatatg gctttacaca
     6961 atagccgaca tgtcttttct cagtcaggaa ttttactttt cctcactacg aagttgacga
     7021 cttcgatatg ggatagactc ttaattcaag caattactct gcacaagcat caggcaatcc
     7081 agctccaat
//
LOCUS       DBFIFS010000224         7013 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.224, whole genome shotgun sequence.
ACCESSION   DBFIFS010000224 DBFIFS010000000
VERSION     DBFIFS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.224"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..514
                     /gene="ykgH"
                     /locus_tag="N7A07_004064"
                     /pseudo
     CDS             1..514
                     /gene="ykgH"
                     /locus_tag="N7A07_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(757..1452)
                     /gene="ykgG"
                     /locus_tag="N7A07_004065"
     CDS             complement(757..1452)
                     /gene="ykgG"
                     /locus_tag="N7A07_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU2478626.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1445..2872)
                     /gene="ykgF"
                     /locus_tag="N7A07_004066"
     CDS             complement(1445..2872)
                     /gene="ykgF"
                     /locus_tag="N7A07_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU2478627.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2883..3602)
                     /gene="ykgE"
                     /locus_tag="N7A07_004067"
     CDS             complement(2883..3602)
                     /gene="ykgE"
                     /locus_tag="N7A07_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU2478628.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4129..4983)
                     /gene="rclR"
                     /locus_tag="N7A07_004068"
     CDS             complement(4129..4983)
                     /gene="rclR"
                     /locus_tag="N7A07_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU2478629.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5209..6534
                     /gene="rclA"
                     /locus_tag="N7A07_004069"
     CDS             5209..6534
                     /gene="rclA"
                     /locus_tag="N7A07_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGU2478630.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6643..6879
                     /gene="rclB"
                     /locus_tag="N7A07_004070"
     CDS             6643..6879
                     /gene="rclB"
                     /locus_tag="N7A07_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU2478631.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6891..>7013
                     /locus_tag="N7A07_004071"
     CDS             6891..>7013
                     /locus_tag="N7A07_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HGU2478632.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEAD"
BASE COUNT         1933 a         1707 c         1510 g         1863 t
ORIGIN      
        1 cctaaaaata aatcagacaa cacccggcat acttgttaac tgtgatagca ataataactt
       61 tgcctgtcag acaacaatga ctgaagatgt tattcagcga attactacat tttttcacac
      121 cagcccagat gtcaagaaca gagaaataaa gctggaatgg tcaggagata agagagattt
      181 accaactgct gaagcggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc
      241 agaataccat aatggcaggt aagttctcga tgagatacaa acgccttcag caattaacag
      301 tgagctttat acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga
      361 tggctgtgta actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct
      421 ggctctggga ttttttctaa gcattgtaat ttctgtaatg ttttgtcttg tcaaaaaaat
      481 ggtagatgaa taccaacaaa actctgggca gtaataaaca gcattctgat agagagtgcc
      541 tgtataaagc tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg
      601 tctttttcac tgtaaatatc ctggcattag taatgccagg atatttacag aagaggagca
      661 taaccatgac ggaacggggt taagtcaggt tgtccttaac gctattttca acctcgtatg
      721 ccttcttcag gtttatgtcc agacttcata cctctctcag caatcctcaa taatcagata
      781 caccgctttc accggaccat gaaccccgac gactttgata agctcaatgt ccgccgttga
      841 gctgggacca ctgatgatgt taatacatga cggcatgcgt tcaccggcct gcgctttctg
      901 atgcaatctt tctgcgagtt gcgctacacg cggcaggata gtgcttttac gcaggataaa
      961 aagagatgat tccgggagca ggctcaatga acgcccacgc tcggcggcgg aaaaaagaac
     1021 cacgcctccc gattcggtta aaccatattc agcatacacg acacccactt tagcctgctc
     1081 tgcctgcgag atattcttgg cacctttcgc cggatcccaa acaacggcat tgcattcctg
     1141 ctgcaaacgt tcgctaatcc ctaattcctc cagcctcgtg tcaccgctaa tcacgaccga
     1201 ctgatctccc agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt
     1261 cagctcacag cgcgtcaaca taacatcgct ggcaaactga ataaacgcgt cacagcgctg
     1321 ctgttggtta agttgggtaa gccgctcgtt agcatagtta ttaagcggcg catcttctgc
     1381 ttgcggttca agtcgcagcg ggcgacccag tgcctgagca acgttattca aaaattcgcc
     1441 tcgattatcc attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct
     1501 ctccgtcagc ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga
     1561 gtggtgtttt gccgccattg ataaaccagc ttgccgcatg agcaccggcc atcatcccga
     1621 ctttccacaa tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg
     1681 cttttgcggt gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca
     1741 gcggaatacg caccggacac acgttgtcac aagctgtgca taaagagcag gcgtagggta
     1801 aatctttaaa atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg
     1861 gataaataga gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac
     1921 aagccccgca gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac
     1981 gcccgttatc gacaataacc agatgaaact cttcaggacc atcaacgtgc cccgcttcgc
     2041 gaggtcctgt cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca
     2101 gcatggtgat caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca
     2161 ctgcaatatg cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca
     2221 ggcataccga acctgtctct gccaccgcga aattacagcc cgtaatacct atttcagcac
     2281 tgaggaaatc ttcgcggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg
     2341 gcccctcata gcccagacgt tcgtgtagca ctcgacggat ctgatggcga tctttatgaa
     2401 ttgccgggac cacaacatga gagggcggat cttgatccag ctggagaata tattcaccca
     2461 aatcggtttc aatcacctga atgccagcat cctgcaacac atgattgaca ccaatctctt
     2521 cggtcaccat cgatttagat ttcaccacct tccgggcatt tttgcgttgg gcaacctgta
     2581 aaatgtagcg ggtagcgtct tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg
     2641 tcactttttc tgagagctgg tacagataag cgtcgagatt actcagaaca tgatcacgta
     2701 tctgggcggc ccgatcgcgc cactcctccc agagccccaa ttcatcgacc attttttgcc
     2761 gatttgcccc aatacgctgc tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa
     2821 tttgctgacg gatgcgtgtc ttaaaatctg tattactggt tttgatcgac atctttatat
     2881 cctcagcggc tcatcaacac ttcagcaata tgcatcactt tgactttctg cccttcccgt
     2941 tgtaatcgcc cactgatgtt cagcaggcaa ctcacgtcag caccaattaa atactcaggg
     3001 cggacttcca tcaggtgcgc aaccttttct ttcaccatct cgccggatat ttcggccatt
     3061 ttgaccgaga acgtgccgcc aaatccgcag caggtatcct gttcagcaaa ggtcaacagc
     3121 tccagtccac gcacattttt cagcagcgta agtggctcgt ccttcacccc cagcttacgg
     3181 gccaggctac aagatgggtg atacaccgct ctcccttgca aactggcacc tacatcgact
     3241 acccctaatt tattaacaat aaaagaggtg agatcctgca tacgcgcggc aaccttttcg
     3301 gctcgtgatg cccattcagg ttcatccgcc agatacgtcg ggtaactttt tacggcatag
     3361 gtgcaagagc cagccggtga aataatggga tcgtcgttat cctccagtgc ggcgatcaga
     3421 tttttcatcc ctggaatcgc ttctttgata taaccactat tgatcgcagg ctgaccgcag
     3481 catccctgtt tctccgggaa atttacgcga cagccgagtt tttccagtag cagcacggag
     3541 tctcgtgcca ttcttgattt cagggcgtca ccaatacagg tgacaaagaa attgacattc
     3601 acaactaata ctccattact tcatgcccat ttattcccga tgtttccccc cacccgggga
     3661 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc accccttatt
     3721 tgtatgacaa atgatagtct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     3781 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     3841 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     3901 tacactgatg gtatacaaaa aaattaccga caataatcac gtatcaagta atagtcggga
     3961 ttttatttaa ttgaagcaat ttagtttgca tttttattta aaaactgagc tagagtccat
     4021 cagaaaaaaa tatataaagc ccaaaccacc gttggaaata gaaatagttg aaaggctcac
     4081 aactcccgga atgcggatta tctttgccct gatttctggc ggttttattc agggtgcaag
     4141 ctgtctgacc ctttcccgat attctcccgg agtacaacca aactcgcgga caaacgcctt
     4201 gtgaaaagat gattcactgg catagcctac tgactcagcg atcaccacaa cagggagcat
     4261 ttcccgggaa aacatctggg ccgctatttg tagacgcaac tttgttaata cagccagcgg
     4321 cgtggttccg gaaacatcac ggaaaagctg ggcaaaactt gcccgggaca tgtgggcgat
     4381 gctggccagc gattcgacgc tccaggcgtg tcctggcatt tccagcattt gctgtattac
     4441 cgcaccaaga cgtggatgca gaagcaaact gagaatgttt ttctcagtat taacctgtgc
     4501 aatccactca cgcaccgcga gggtaaagaa tgtagcgcag atctggctac acagtgcatc
     4561 cactcccggc attgccgatc tggattcctg ttgtaaaaac gggatcgcct ccctcagcca
     4621 gttatattcc acgctgtggt taaccggtgc taaaaacagc gtttccggca aagacgttaa
     4681 aaaataacgt gccgaatgtt gcagccgaag agtaccacag acaatacagg tcgattcatt
     4741 atcgacatga ctcaggcgat gagctgaatt ttgtggtagc aggaccacat ttcccgggcg
     4801 taatgtaaaa atctctcccg tcggcatttc cagcttcgcc gctccttgcg ttaacgcatg
     4861 ccaacgaata actgataatt ccccggcacc atgtggaagc tgccagtcac ttcctaacac
     4921 gcaattctta tcgatcgttc cttgtggagc gttaagcatc aacaaacggc taagggcatc
     4981 catatcagac tcctgagtgg tttttgatta caaacaccaa caatcttagt aaacaactaa
     5041 tctaatacca tcaaatagtt aatcatgatt tttgttttgt gattctgatt aagaaaaaga
     5101 aaataccaga cgataagaca aaatatcaag cgttgtgaag aaatgttatt tgctcttttg
     5161 cgtctatagt catgatgtca aatgaacgcg tttcgacagg aaatcatcat gaataaatat
     5221 caggcagtga ttattggttt tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa
     5281 gcaggttggc gagtggctct catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt
     5341 aatatcggct gcatcccaac caaaacattg gttcatgacg cacagcagca cacagatttt
     5401 gtccgagcca tacagcgtaa aaatgaagtg gttaattttt tacgtaataa gaattttcat
     5461 aatcttgcag atatgcccaa tatcgacgtg atcaacggcc aggcggagtt tatcaataat
     5521 catagcctgc gtgttcatcg gcctgaggga aatctggaaa ttcatggcga gaaaattttt
     5581 attaataccg gtgcacaaac cgtggttccg ccaattcctg gaattaccac cacgccagga
     5641 gtatatgaca gcaccggatt acttaatcta aaagaattgc ctgggcattt aggtattttg
     5701 ggcggcggat atattggcgt tgagttcgcc tctatgttcg ctaattttgg cagcaaggta
     5761 accattttag aagcagcttc gctgtttttg cctcgggaag aacgggatat tgctgataat
     5821 atcgcgacga ttttacgcga tcagggcgtc gatattatcc tcaatgccca tgtggagcga
     5881 atcagtcacc atgaaaatca agtgcaagtg catagcgagc acgcccaact ggcggtggat
     5941 gcactgttaa tagcttccgg tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc
     6001 ggtatcgcag taaacgagcg cggggcaatt gtcgttgaca agcgattaca taccaccgca
     6061 gacaatattt gggcgatggg agatgttacc ggcgggctgc aatttactta catatcactg
     6121 gatgattacc gcattgtacg tgatgagtta ctgggtgaag gcaaacgtag tactgatgat
     6181 cggaaaaatg tgccttattc cgtatttatg acaccgcccc tgtccagggt tggtatgaca
     6241 gaagaacaag ccagagagag tggtgctgat attcaggtgg tgacattgcc tgtagctgca
     6301 attccgcgcg ccagagtgat gaacgatacc cgtggggtat taaaagcgat tgttgataat
     6361 aaaacccaac gtatattagg ggcatcactg ctgtgtgttg actcccacga gatgattaat
     6421 atagtgaaaa tggtgatgga tgccgggctg ccttatagca tattacgcga tcagatattt
     6481 actcatccgt cgatgagcga atcactcaat gatctatttt cattagtcaa ataaactcaa
     6541 aatcagacgc cagaacaaat attctggcgt ctcagagaaa agaatcttat taattccctg
     6601 ttactctata acttcttaat cacttcattg atggtatttt atatgtttaa aaaatccgtt
     6661 ttatttgcaa cactattatc tggcgttatg gcattttcca ccaatgcaga tgataaaata
     6721 attctgaaac atatcagcgt ctcgtcagta tcagcatcac cgacagttct ggaggatgcc
     6781 attgctgata tagccagaaa atataatgct tcatcctgga aagtcacatc gatgcgaatt
     6841 gataataatt caaccgcaac agcagtattg tataaataag gatattcaca atggaaaaat
     6901 acctgcacct gttaagtcgg ggagataaaa ttggcctggc attgattcgt ctgagtattg
     6961 cgattgtttt tatgtggatt gggttattaa agtttgtccc ttacgaggca gac
//
LOCUS       DBFIFS010000225         7010 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.225, whole genome shotgun sequence.
ACCESSION   DBFIFS010000225 DBFIFS010000000
VERSION     DBFIFS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.225"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            69..1402
                     /gene="adeQ"
                     /locus_tag="N7A07_004072"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             69..1402
                     /gene="adeQ"
                     /locus_tag="N7A07_004072"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1455..1907
                     /gene="yicN"
                     /locus_tag="N7A07_004073"
     CDS             1455..1907
                     /gene="yicN"
                     /locus_tag="N7A07_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU2478633.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2023..2346)
                     /locus_tag="N7A07_004074"
     CDS             complement(2023..2346)
                     /locus_tag="N7A07_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478634.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2330..2683)
                     /locus_tag="N7A07_004075"
     CDS             complement(2330..2683)
                     /locus_tag="N7A07_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2478635.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2917..4107
                     /gene="nepI"
                     /locus_tag="N7A07_004076"
     CDS             2917..4107
                     /gene="nepI"
                     /locus_tag="N7A07_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU2478636.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4148..4441)
                     /gene="yicS"
                     /locus_tag="N7A07_004077"
     CDS             complement(4148..4441)
                     /gene="yicS"
                     /locus_tag="N7A07_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU2478637.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4663..5481
                     /gene="nlpA"
                     /locus_tag="N7A07_004078"
     CDS             4663..5481
                     /gene="nlpA"
                     /locus_tag="N7A07_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU2478638.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5485..6408)
                     /gene="yicL"
                     /locus_tag="N7A07_004079"
     CDS             complement(5485..6408)
                     /gene="yicL"
                     /locus_tag="N7A07_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU2478639.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6519..6692)
                     /locus_tag="N7A07_004080"
                     /pseudo
     CDS             complement(6519..6692)
                     /locus_tag="N7A07_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6927..>7010
                     /locus_tag="N7A07_004081"
     CDS             6927..>7010
                     /locus_tag="N7A07_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478640.1"
                     /translation="MCGGQKKADLPLKMQVAKVVKNAPGEAE"
BASE COUNT         1703 a         1570 c         1758 g         1979 t
ORIGIN      
        1 tcactgaacc gttgttgtca tttgttttac aaaagcaagg gattaaatct cctccaatgg
       61 acaaaaaaat gaataatgac aataccgatt acgtgagtaa tgaatcaggg acgctttcgc
      121 gattatttaa actacctcag catgggacca ccgtccgcac agaattgatt gcggggatga
      181 ccactttttt aaccatggtg tacatcgttt ttgtgaaccc gcaaatcctc ggcgcggcac
      241 aaatggaccc gaaagtggtg tttgttacca cctgtttgat tgccggtatc ggcagtattg
      301 cgatggggat atttgctaac ttacccgtgg cgctggctcc ggcaatgggg ctgaacgcct
      361 tctttgcgtt cgtggtcgtg ggggcgatgg gtatctcctg gcagaccggg atgggcgcga
      421 tattctgggg cgcaattggg ctatttttgc tcacgctatt tcgtatccgg tactggatga
      481 tctccaacat tccattaagt ttacgtattg gtatcaccag cggaatcgga ttatttatcg
      541 ccttaatggg attaaaaaat actggcgtta ttgtcgccaa taaagacact ctggtgatga
      601 ttggcgattt aagttctcac ggcgtgttgt taggtatttt agggtttttt attataaccg
      661 tgttgtcatc acgtcatttt catgccgcgg tgcttgtttc tattgtggtg acgtcttgct
      721 gtggattatt tttcggtgat gttcatttta gcggcgtcta ttccattccg cctgatatta
      781 gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac acttgaactc gccggtatca
      841 ttttctcctt tatgctgatc aacctatttg attcatcagg aacattaatt ggtgtaactg
      901 ataaagctgg cttaatagat agtaacggta aattccccaa tatgaataag gcgctatatg
      961 ttgatagcgt agttcggtgg cgggcgcgtt tatcggcacc tcgtctgtta ccgcctatat
     1021 tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg ctgactgcgg ttgtggtcgg
     1081 cgttatgttc ctgttggtta tgttcttctc accgctggtg gcgatggttc ctccttacgc
     1141 aaccgccgga gcgttaatct ttgttggcgt gttgatgacc tcaagtctgg cgcgcgttaa
     1201 ctgggatgat tttaccgaat cggtgcctgc gtttgttacc acggtgatga tgccctttac
     1261 tttctcgatc accgaaggga ttgcacttgg ctttatgtcg tactgcatca tgaaagtgtg
     1321 caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg gtcgcagctc tgtttgcact
     1381 gaagattatt ctggtggatt agtaactggc gagggtttcc agatatcatg agttctgatt
     1441 acgcaggaga actcatgatc tggataatgc tcgccacgct ggcggtagtg tttgtggttg
     1501 gttttcgggt gctgacatcc ggggccagaa aagcgattcg ccgtctcagc gatcggctga
     1561 atatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat gggaaagtca gccggtgacg
     1621 aatttttacg ttatttgcat cgtccggatg agtcgcacct gcaaaacgcc gcgcaggtgt
     1681 tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca gaacctgctg caatggcatc
     1741 ggattttaca aaaagcccgc cttgccgctc ccattactga tgctcaggtc aggctggcgc
     1801 tgggttttct gcgcgaaacc gaacctgaaa tgcaggatat taatgctttt cagatgcgct
     1861 ataacgcgtt ctttcagcct gccgagggcg ttcactggct gcattaaaac cgcttcgtct
     1921 tatcagccta cagaccgcgc ctgacctgta gggtggataa ggcgttcaca ccgcatccgg
     1981 tatcggaact gacagtaggt gaacgcatta tctacgttct atttacagca gcacctccaa
     2041 cccgtgtttt tgcacaatgc tgagcagttt taatgaaatg ccattggggc gtttagcgcc
     2101 actttcccat ttttgtacag ttgaaacgct ggtattgaga tagtgagcga agacgggctg
     2161 actcactttt aatttttccc gcagtgcttt tatctcaaca ggttgtaagt cttgcacgct
     2221 ggtcaggcag gctttgtcaa aagtacgcat cgtttcagca gaaatagcat tgacgctgga
     2281 caatccagcc gctgcgctgt gaatagcctc aaatgcagga cttttaaatt tagtttttgc
     2341 agtcatagca gatctctgtc agttctttag tgttaatcat tgctgttagt tgggctttcg
     2401 tcaggaaggc ataatgcttt gcaatttcac gaaacccagc cagttcctga ctattgatgt
     2461 tcgacatatc ctgttttgca tataaaaacg tgtaaaacca attgcttacc cctttcgcca
     2521 gaataattgc acgctcacga ttttggtgaa ggcgcttttt aaaaacgcca ccgccaagtg
     2581 catcagcttt accttgttct gtttctaaaa tggcccggca caactcttca tctgttattg
     2641 catgagacct ggcttccctt gcaaaccatt tggttttaaa cacgcgtata tcgttgaggt
     2701 cctgttatta aagtatatca ccaagtgcaa tactttatcc cttgttccca acacaatcgc
     2761 gaaaatacat atcaccttgt cctatgattt ttatttcttc gagcagcctc gagcttttcc
     2821 cacaacaccg taaaagttgc aaaaacgtta aatgcgtcac acatttcaat gttaaagtgg
     2881 tcggcttttc ccctgaaaca tgccacgggt aacaccatga gtgaatttat tgccgaaaac
     2941 cgcggcgcgg atgccatcac ccgaccgaac tggtcagccg ttttctcggt ggcgttttgc
     3001 gtcgcctgtc tgattatcgt tgagtttttg cctgtcagtt tgttgacgcc aatggcccag
     3061 gatttaggca tttcggaagg ggttgccggg caatcggtga ccgtgaccgc ctttgtggca
     3121 atgtttgcca gtttgtttat tacccagaca attcaggcca ctgaccgccg ctacgttgtt
     3181 attttgtttg ccgttttgct gacgctctcc tgcttgctgg tttcctttgc taactcattc
     3241 agtttgcttt taatcggtcg tgcctgtctg gggctggcgc tgggcgggtt ctgggcgatg
     3301 tcggcgtcgc tgaccatgcg tctggtgccg ccgcgtacgg tgccgaaggc gctgtcggtg
     3361 atcttcggcg cggtttctat tgcgctggtg attgccgcgc cgttgggctg ttttttaggc
     3421 gagcttatcg gttggcgcaa tgtctttaat gcggcggcgg cgatgggtgt gctgtgtatt
     3481 ttctggatta tcaaatcact gccttcgctg ccaggtgaac cttcgcatca gaaacaaaat
     3541 actttccgct tattacaacg tccgggagtg atggccggga tgatcgccat ctttatgtcc
     3601 tttgccggac agtttgcttt tttcacttat attcgcccag tgtatatgac tctggcggga
     3661 ttcggtgttg atggcttaac cctggtgctg ctgagttttg gtatcgccag ctttgtcggg
     3721 acatcgcttt cgtcgtttat tctcaaacgt tcggtgaaac tggccttagc gggcgcaccg
     3781 tttgtgctcg ccctgagcgc gctggtactg acgctgtggg gaagcgataa aattgttgct
     3841 actggtgtgg cgattatctg ggggctgacc tttgctttaa ttcccgttgg ttggtcaacg
     3901 tggatcactc gctcattggc cgatcaggca gaaaaggctg gatctattca ggttgccgtt
     3961 attcagcttg ccaatacctg cggtgcggca atcggcggtt atgcgttgga taacattggt
     4021 ctgacttcgc cgctgatgtt gtccggcaca ttgatgttgc tgactgcatt gttggtgact
     4081 gcaaaggtga aaatgaagaa atcctgatat caggtaaaga agcgttggtc agtggttgtt
     4141 gccgttctta catatccggg cattctacct tacccaacgc ttcccaataa ttgctctgat
     4201 tattacgttc catcgccacg atggcctcgc gtatatgttg tttattgttt tcatctgaca
     4261 acattaactt ttcccacgct tcatctgata atgacgcctg tggcgtgcaa attttacgta
     4321 aatcttgcaa taccgtggtt ttctcttttg ccgactgtag agaactgaat ggcgactcgg
     4381 cctgagaaag ggctggaaaa atcaggaata cggtaatcat gagtagcatc gttggcttca
     4441 tagcggcaac cttctgcaaa tgtgcatcga atgaagcata agtgtagttt gcttttgccg
     4501 ctctcatcat gctgtacgaa aggtgtttgc gtggtcagta agaagtgcca gactttatat
     4561 tccactttta ttccttttta ttcttatcga tagcgttttg ttttttaaac tgcagcgacc
     4621 ttaccgctat agtccggtaa tcattaataa aaggataaaa aaatgaaact gacaacacat
     4681 catctacggg cgggggccgc attattactg gcaggagttc tgctggcagg ttgcgaccag
     4741 agcagcagcg atgaaaagca tattaaagtt ggcgttatta atggcgcaga acaagatgtc
     4801 gcagaagtcg ctaaaaaggt agcaaaagag aaatatggcc tcgatgttga actggtagga
     4861 tttagcggtt cgttgctacc gaacgatgcg acaaataatg gcgagcttga tgcgaacgtc
     4921 ttccagcatc gaccctttct tgaacaagat aatcagacgc atggttataa actggtagcg
     4981 gtgggaaata cgtttgtctt ccctatggcg ggctattcca aaaaaatcaa aaccgtggcg
     5041 caaataaaag aaggtgcgac agtggcgatc cccaacgacc cgactaacct tggtcgcgcg
     5101 cttttactgc tgcaaaaaga gaagctaatt actctgaaag agggaaaagg gttattacct
     5161 accgcgttgg atataaccga taatccacgc catttgcaga taatggagct ggaaggggca
     5221 cagttgccac gtgtactgga cgacccgaaa gtggatgtag cgattatcag caccacttac
     5281 atcgagcaaa caggactttc tccggttaac gacagcgtat ttattgaaga taaaaattcg
     5341 ccgtatgtga atattctggt ggcacgggaa gataataaga atgcagaaaa cgtgaaggaa
     5401 tttctgcaat cttatcaatc acccgaagtc gctaaagcgg cagaaaccat ctttaacggt
     5461 ggtgcggtgc ctggctggta attctcactc atgccgcgcc ggacgattta tttttctggc
     5521 gcggcggcgg gaatccattg aaatcaaaat caccgatgac agaatcagca gcgttcccag
     5581 ccagtccggc aatgtgaagg tgatccccag caacagcaaa gagagcagcg cgctacttaa
     5641 tggttcagca cagcttaaaa tgctggcttt tggaccgcca attaattgtg ctcctttcag
     5701 gtacagacta aatgtcaggg acgtaccaat gaccaccaaa taaaaaaacg ccagaatcaa
     5761 acttccgtta acgataaagt ttgtcccttg tccggcataa aagggcagca ggatcagacc
     5821 gccaatgagc atactccagc cgacgaccgg taacgtgcca tagcgggcaa tcagcgttga
     5881 gggataggtg gtatagaatg cagcagtaaa tgccgaggca atgccccaga ataacgcggc
     5941 aggagagatc gataatgacg tcggattacc gtgtgtcacc aataaaaaag tcccgatcag
     6001 cgatgtcaaa atagcgcaga aaaccagaat gcccgggcgg gatttacgca ccagtgagaa
     6061 ccaggcgacg ataatagtcg gtgagaggaa ttgcagcacc gttgccgtgg ctgcgttcga
     6121 tttttcgatg gttagcaaaa aagtgagctg tacggttagc gcgccaacca cggaaaaaat
     6181 cagtaggcta atggcatctt tgtgattgtt aataatagaa aagattttat caccatgaac
     6241 aaatgatagc gtcagcagaa tcaaaccggc gaatatcaaa cgcgtcatag tcaaaaactg
     6301 cgacgacatc tggctttgct ccatgatgta ttgcgcgcag acccctgaac ttccccacaa
     6361 cacggcggca atcagaacgt tcagcatccc ctttctggtg gaacccattt ttccctctgc
     6421 atactgtttt gtcgtttatg tatgcaggca tgatagcaaa aatggcgagg atggctcagt
     6481 ttgcgccacg ccgatttttc ttagcccaga ctggtgagct aaatatcttt aataaacagc
     6541 aagcaaatca ttgcaatacc caacatccct atcgccagcc agaacaacgt gtggtagctc
     6601 cagatttcaa ccataatttc gtcaacagag ccggcgataa tctagccgaa gcgtgaggta
     6661 tttgcatata acgttgttgc ggaacctatt cttatttatc ggagtgcctt gccagtaatt
     6721 tactgacgag gatcctcata atagcgttac ccatctgttg cattgagact ttacaggccg
     6781 ttattctaaa tgaccaaaat taaatcagtg cataagggga agtattctgt agtgatgtct
     6841 ccctgagatg caggttccac tggagaaaat tatgagcagt ttgtttcaga atgaaataaa
     6901 ggataaattt aaaactggac ttgcatatgt gcgggggcca gaaaaaagcg gatcttccgc
     6961 tcaaaatgca ggtagcgaag gtggtaaaaa atgcgccggg agaagcagaa
//
LOCUS       DBFIFS010000226         6943 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.226, whole genome shotgun sequence.
ACCESSION   DBFIFS010000226 DBFIFS010000000
VERSION     DBFIFS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.226"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..603)
                     /gene="yeeO"
                     /locus_tag="N7A07_004082"
     CDS             complement(<1..603)
                     /gene="yeeO"
                     /locus_tag="N7A07_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU2478641.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLN"
     gene            764..839
                     /locus_tag="N7A07_004083"
     tRNA            764..839
                     /locus_tag="N7A07_004083"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:797..799,aa:Asn,seq:gtt)
     gene            complement(877..1827)
                     /gene="cbl"
                     /locus_tag="N7A07_004084"
     CDS             complement(877..1827)
                     /gene="cbl"
                     /locus_tag="N7A07_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU2478642.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1929..2846)
                     /gene="nac"
                     /locus_tag="N7A07_004085"
     CDS             complement(1929..2846)
                     /gene="nac"
                     /locus_tag="N7A07_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU2478643.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3172..3247
                     /locus_tag="N7A07_004086"
     tRNA            3172..3247
                     /locus_tag="N7A07_004086"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3205..3207,aa:Asn,seq:gtt)
     gene            complement(3303..4238)
                     /gene="ldtA"
                     /locus_tag="N7A07_004087"
     CDS             complement(3303..4238)
                     /gene="ldtA"
                     /locus_tag="N7A07_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2478644.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4300..5379)
                     /gene="cobT"
                     /locus_tag="N7A07_004088"
     CDS             complement(4300..5379)
                     /gene="cobT"
                     /locus_tag="N7A07_004088"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU2478645.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(5391..6134)
                     /gene="cobS"
                     /locus_tag="N7A07_004089"
     CDS             complement(5391..6134)
                     /gene="cobS"
                     /locus_tag="N7A07_004089"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU2478646.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6131..6676)
                     /gene="cobU"
                     /locus_tag="N7A07_004090"
     CDS             complement(6131..6676)
                     /gene="cobU"
                     /locus_tag="N7A07_004090"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU2478647.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6828..>6943)
                     /locus_tag="N7A07_004091"
     CDS             complement(6828..>6943)
                     /locus_tag="N7A07_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGU2478648.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         1872 a         1878 c         1527 g         1666 t
ORIGIN      
        1 attcaggcta ccgttaatca gtagcggtat tttcgtattc cctgcaccac gaagtgcccc
       61 gctaccaatt agagtgatgg cagctgctgg ataactgagt accgtcagct ccagataagt
      121 caacgccagt gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa taatttgctc
      181 gccaaaatga tgaataagcg ttgccaacag tacggcaaac aacgtcatga tcaccaatga
      241 ctgccgcgtc gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa atgccacaac
      301 gacagtagta ccaagatcga tagcagcaaa aaaagccata atgaccatat tgaagctgtc
      361 cgccaatccc acgccggcca tcgcatcttt tcccagccag ctgaccagaa aagtgctcag
      421 aacccccatc aacaggacac aggcattctc catgaagata ggaacagcaa acggggttat
      481 ctcgcgccag aacaacactt tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac
      541 aacctggcgt aaagcagagg agatattcaa agccgacctt aattgcagaa agtgaaacca
      601 catttcaaat aatgagggag aatcagcaaa gctgcaaaga ttttcgccat caaattgtct
      661 gcaaatgcaa caaactgttg atagaaacgg caaacagttg ggaaaattaa aaatcgggtt
      721 tgacaaaaga tttttcgccg ttaagatgtg cctcaacagc gattcctctg tagttcagtc
      781 ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa
      841 atttctgaaa agcccgcttt tatagcgggc tttttgctat atctgataat caatttcctc
      901 ttcactgttt tccatcacct gccgcttgat atcctcaact gaaagtcctg cattgcaaag
      961 ttccagaaag cgccagacat agttacgctg aagttgtcct cgcttcagtc ccaaccagac
     1021 agtattagca tcgaaaagat gccgcgtatc caggcggatt aaattctttt cctcttgttc
     1081 accgctggat tgttcggcaa ctaatccgat cccaagccca agagcaacat aggttttaat
     1141 gacatcagaa tcctgcgcac ttaatataat atctgccagc aaacctttgc gggcaaatgc
     1201 gtcatcaata cgtgagcgcc ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc
     1261 tattgattcc agcgtcaatg gtgtaatttg cgtcaaggga tgatcgtgtg gaacaagcaa
     1321 actatggtgc caacgaaacc acgggaaggc gacgagctgc gggtcattac tcaaacgctc
     1381 gctggcgata ccaatatcag cttcaccatt atgcaacaac gtcgcaattt cctgtggcgt
     1441 cccctggatt agctcgagcc gaacctccgg gaaaagttcg cgaaaagctt taatgacctc
     1501 tggcaagcta taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc
     1561 gttggtaaac aggtctgcaa gccgacgaac attactggct tcattgagaa tacgttctgc
     1621 aatgaccagt aatgctttgc ccggttcagt catgcccagc agtcgcttac ctcgtcgaac
     1681 aaatatttcg atgccaagtt catcctccag ttcccgaata tgacggctga cgcctgactg
     1741 tgaggtaaaa agcatattcg caacctctgt caggttgtaa tcctgacgtg cagcttcgcg
     1801 gattatcttt agttgttgga aattcacggt aaactccggg cagttcagat atcccgttat
     1861 tgttaaagtc taacgcccgg cataacaaat aataaaaacc cgcatcttat tccatcccga
     1921 tataacgctt agctcaccaa ttgccactgc cttttttcca tcactggaga actaatcact
     1981 gacattaaca actctttcac tgcctgtgcc tgtggcgata agttcgctct ggcgggtaaa
     2041 tttaatgaca aagagagact catggaaggc gtggtaatgc gtgacatcca cccatttact
     2101 gcgccacata acgaacgcgc ggccgattcg ggtaatactg caacgcccat gccgctggca
     2161 atcgctgcgg taagcgtagc aatagactca atttcaccaa tgacttttgc cgtgagccgc
     2221 cgtagggaaa acgcctcatc aacacgaagt ctaacagcgc tgtaatcact ggggagaaaa
     2281 aggttcattt gtgcaatagc attaacatca acgctttgcc ccgggcaatc ttgagttcct
     2341 accaggaaaa gatcttcttt cagcaaagcc tgactggata caccagccac aggggaatgc
     2401 tcataaatca ccgccatatc gagttggtga tttatcagtt tttcgttaag cactgcaccg
     2461 ctattttcat gaagatagat aacgacctcc gggaattcag cgcgaaccgc ctgtaataag
     2521 ggcatggtga tggatgacgc agcggttcct ggcgcaaagc caatcgagac ttgccccgat
     2581 aatgcctgac caacgttatg caccgccagt tgggcctgct cacactgacg taaaatggcc
     2641 cgcgcatggg tatagagaat ttttccggcg tctgttggcg taacgccccg ttttgtgcga
     2701 atcaaaagtt gttgatttaa ctcaccttcg agtgtggcaa cctgctggct gagcgctggt
     2761 tgtgcgatat gcaatacttc agcagcctga gtcaggctac caatatctac aatttttacg
     2821 aagtatttca ggcgtctgaa gttcatgttg cctccggttt ttgagaatcg gcccaagtgc
     2881 cgccattact tacaaccaga ttgcaagatg cttgccagtt ttattttggc gttgatgtac
     2941 aagctaacca actgtcaaat aagagattat gatagattcg tcattggctc ttttcaccag
     3001 cagtcgcgtt cccctgccct ataaaaggag ggtatgcacc acgatggttc attaccaata
     3061 agattgaaat ctcatcactt tgttgaaatt gacagcaaac aaacaaaaaa atgcatttca
     3121 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     3181 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     3241 ggagccaaat tcaaaaaagc ctgctttcta gcaggctttt tgctttctaa ttaccaacgc
     3301 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgacgttgc
     3361 aacgcagcat ttgcgcgatt cacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     3421 ggggttaccg gcaatggcac ttttcggtca gactcatatt cagcacgatt gcgcgacagc
     3481 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     3541 tcaataattt gcacacgagt acctacagga acattatcaa acagatattt gatatcgtca
     3601 ttgcgcagac gaatacagcc ctgacttacc cggagcccaa taccaaaatt ggcattggta
     3661 ccgtggatgg catatagcct gccaatataa atcgcgtaca gccccatggg attatcgggc
     3721 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     3781 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     3841 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccacagt attactgtct
     3901 ggtgggtagt aataaagacg catctcagcg acgttaacca caatcccttt acgaacagtg
     3961 acgggcaaaa tcagttgctg cggaatggtg agttgcgaac ctgacttcgg taagaagaca
     4021 tcagcgcccg gattcgcttc cagcatgtta cttaaccctt gcccgtattg tgcggcaaaa
     4081 gtctccagcg gctgggtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     4141 cggctaccct ctggaggtaa tggataagtt accgccaggc tagtatggct ggcaaaaagc
     4201 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaaa
     4261 gctatcggtt aagtatagct tttatcagac ttttcgtttt taactgttca aatcagaagt
     4321 cgtattcccc ggtagaacaa ttttactggc agcaagttcg cccatgttgt tgtatatcgc
     4381 acaagcagct tcgatgatgg gcatcgccag agctgcgcca ctcccctcac ctaaacgcat
     4441 atccatattg agaaaaggct ccagccccaa atgcgacagc gctatacgcg cccctttttc
     4501 tgccgacagg tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     4561 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     4621 gcctaacatc acgccagcca ttccgaccaa atcaaatcca cccacttttg ccagaacatt
     4681 aacgccatcc tgcggatttg gctgattcaa cgtaatcgcc cggcgcacaa catcaatttt
     4741 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     4801 gccagtgatt gtgctgacta ttgctgctgc cggtgtcgtg tttgccatcc ccagttcacc
     4861 tacaccaaac agcgtgacac cgttttttgc cagctcccgc gtataacata tgacgtccaa
     4921 aagtaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     4981 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     5041 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccgcg
     5101 ggtcatattt tcagcctgaa tggctgtcac ttcttttggg gaaatagcga ccccttcctc
     5161 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     5221 attcaacccc ggcattcccg ccagttgtac gccaagcgcc tccagccttc ccaggctacc
     5281 aacaggtttg agtaacccgt caatatgccg ttgtgcacgc gacatagcgg caggatcgat
     5341 ggcagggatc gtattcagta aattggcaag tgtttgcatg tcacgtctct ttatagcagt
     5401 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gccggtttgc
     5461 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     5521 atagcagcca caccatgcat acccggcaat aatacggcag caaaaatagc ggctaagccg
     5581 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     5641 tcccgggcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
     5701 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaaa
     5761 atctttgcca gtacaacaaa aatcaatgcc agaccgccgt gggtacctaa acggctatca
     5821 cgcatgattt ccaacatgcg atcgcggcta cgtgcagaaa atacgccatc acaggtatca
     5881 gcgaggccgt ccagatggaa tcccccggtc atcagcacca gcacaagtac gctaaacagt
     5941 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
     6001 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
     6061 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
     6121 aataatttac tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
     6181 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
     6241 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
     6301 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
     6361 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
     6421 ttgtggtaac gcattcaagc aacacaacct cattcgggtt aatgtctgca tgaattaatt
     6481 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggcct tgccgatgat
     6541 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
     6601 gtgaagagtc cccaataagc gcctctgcgt ggcgactctt cccgctccgt gcgccccccg
     6661 tcaccaaaat catcataccg actcctgatt caaatgtatg atggtattca ttgaacagaa
     6721 caatggatcc cccaccgttg ctgaccgcaa aacatttacg cgattaatac tgcgcgtaat
     6781 ataattaaaa atccagcatt ctcaatccat tacgaaataa gatggcatta cggaatagcc
     6841 agactttctc gtgttaacgt ccgacaaaat gcgtcattga cagcggtttc tgtggattga
     6901 cacatttcat tcagataatg aattaatgct actgccggaa caa
//
LOCUS       DBFIFS010000227         6903 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.227, whole genome shotgun sequence.
ACCESSION   DBFIFS010000227 DBFIFS010000000
VERSION     DBFIFS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.227"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(239..724)
                     /gene="sixA"
                     /locus_tag="N7A07_004092"
     CDS             complement(239..724)
                     /gene="sixA"
                     /locus_tag="N7A07_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU2478649.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(927..3071)
                     /gene="fadJ"
                     /locus_tag="N7A07_004093"
     CDS             complement(927..3071)
                     /gene="fadJ"
                     /locus_tag="N7A07_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU2478650.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TPYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="N7A07_004094"
     CDS             complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="N7A07_004094"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU2478651.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4561..4845)
                     /locus_tag="N7A07_004095"
     CDS             complement(4561..4845)
                     /locus_tag="N7A07_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU2478652.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5217..6557
                     /gene="fadL"
                     /locus_tag="N7A07_004096"
     CDS             5217..6557
                     /gene="fadL"
                     /locus_tag="N7A07_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU2478653.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1735 a         1845 c         1620 g         1703 t
ORIGIN      
        1 agacacgaaa atatcatttc ttttttatta ttaactgttc tattagtttg atgctttgta
       61 aaaatacatt acaacttagt ctcatttcgt cagaaattaa tagggaagca gaggaaaaag
      121 tgcgatgaat tctgtatcga agcgtatcaa ctccgtgaag ggttttgagt gaatttctac
      181 tattgcaaaa agacaaatgc ccgatgtaca gcttacatcg ggcatttttt caaacgaatc
      241 agatagcttt cgccatcttc aaattacatg ggctcatctg ccagttgaac tcgccttttc
      301 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      361 ccggacataa ctcggcgacc agatacccaa ctaacggtaa gtgggagata accaacaccg
      421 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      481 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tccccaactt
      541 cctccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      601 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccgtta gtggtcagag
      661 gacgaaccga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      721 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
      781 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      841 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacaatc cgccattcag
      901 cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
      961 ctttccccac gcgcacccat ctcgactaaa cgctcgcaag gggtaaaacg tgaaccatac
     1021 ggcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1081 cgaaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
     1141 tcacgcacgc tacggataac ctgctcatca acacaacgta cagcttcatt cagcatcaac
     1201 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1261 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1321 tagaaacctc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1381 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1441 ccggtgtcga ttcctacctc atccaaaagt tggattggac ctaccggaaa accaaatttc
     1501 actagcgcgg catcaatgtg cgctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1561 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1621 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg tgctgatgta
     1681 cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg
     1741 ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg taaagatgac
     1801 gtattcgaag caaagatggt atgagcggcg caattttgct caacttccgc caccatctgt
     1861 tgtttcaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     1921 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     1981 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     2041 ccctgcgggt tgatatcttt aattctgacc ggaatccccg ctttacaagc agtgacataa
     2101 gcaataccgc cgcccatcaa gccaccacct aaaatcccca cgctgtttaa tggcgcaggc
     2161 ggcgcatcac tgccgggatc tttcttcact tccgtactgg caaaaaagat actacgcaaa
     2221 gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca
     2281 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2341 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2401 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2461 ttctttgcca gctcaacagc ggcttccagc agaatggagt gcggaacaac atcatccacc
     2521 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat
     2581 gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2641 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2701 tggcacgcca gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata
     2761 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagagct
     2821 tccgcttctt gcgccgtttt gcaattgccg atcatgttga tgtctgcgcc agcaatgaag
     2881 ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc
     2941 ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc
     3001 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
     3061 gtcatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3121 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3181 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3241 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagcgcttca cgagcaaaac
     3301 gttcactacc cagtagctga atattggcca acgtctgggc ggcaaaggct tcgtgcatat
     3361 caatcaatgt cagatctgcc attgtcaaac cggcacgctc cagcgccagc ggtgttgacc
     3421 aggctggacc gagcaacatg tcctgccaga catcgatagc ggtaaatgcg tagctgcgca
     3481 gatatcccag cggtaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3541 ccgctgcgcc atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttacgat
     3601 caaacgccgg acgcagcttt gcgaaatcag caagcgagga attaccgcga atattgttgt
     3661 cttccgcgag cagttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3721 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3781 gttctcgggt gatgccgtag gttttcgcca tttgctctgc agtgtcgccc atccgcaagc
     3841 cggtagaata ttctgctacc gcaggtggta cgggcattaa gtcgcgcaaa cgcaggcgag
     3901 agaagagttt cagacgctgg ctcattgtgc gggctttgtt gacatcaacc agcacgcgcg
     3961 ccagtttttt actgacgcca attggcaata ccgaagagga atctgcccca ccggcaatcc
     4021 ccgctcggat agtccccgcc atcaggcttt ctacgacgtt tgcaaccgcc tggaaactgg
     4081 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattcca gtaccgagaa
     4141 caatttcacg cgcaatgttg ggagcttcag gcatttgtac gacctgacca aagaccagtt
     4201 gttcaatcac ttcggcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4261 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4321 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca
     4381 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
     4441 tttttacatt tagccaagcg gagaaaaggg aaattgggag ctatgacaca gtgaagacag
     4501 gagaaagaaa agaaaacgcc cctacctgtt gctggcagga gcgtacttat ccgagaggaa
     4561 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4621 atccaggcgc acaccatcag actcttcagt gaaagtcggg gagattttgc acggttcgga
     4681 ttccacgcta cgggctttct cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
     4741 tgcaaacacg cggctgtaag acgctgtgca gtcggagttg tccataatgg tgccaacatc
     4801 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4861 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     4921 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     4981 aaaacaaaat tcacccgaat ccatgagtgc gccacctcca aattttgcca gctggatcgc
     5041 gtttcttaga tcatatttga aaaaagatag aaacatactt gcaacattcc agctggtccg
     5101 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5161 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5221 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5281 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5341 attcagggga aggcgcaatt gccgatgatg caggtaacgt cagccgtaac cccgcattga
     5401 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtaa
     5461 atatcagcgg aacgtctcca tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5521 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5581 ctattacctc taactatggc ctggcaacag agtttaacga tacttatgca ggcggctctg
     5641 tcgggggtac aaccgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5701 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5761 gtttcgcagg cgatctgggg cagctggttg ctggtcagat tatgcaatct cctgccggga
     5821 agactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     5881 tcgctcatct gaacggcaac cagtggggct ttggatggaa cgccggtatc ctgtatgaac
     5941 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     6001 gtaactacag cagcgatctt aatcgtgtgt ttaataacta cggtttgcca attcctaccg
     6061 ccacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
     6121 tgtcgggtta taaccgtgtt gatccgcagt gggcgattca ctatagcctg gcttacacca
     6181 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6241 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6301 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gcacagaatc
     6361 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcgt
     6421 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6481 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6541 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact
     6601 ttaactcccc cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa
     6661 cagcctgatg gcagctagcc ctttcaaatc agtaagtggt atccacagac aaacctgaag
     6721 ggaaacgcat attttcaggc gttctgctcg tccttctcaa agagtttact tttctgaatt
     6781 tccaggatac tcaaccccct ggctattgtg cgctcataca cgcaaattaa agataggttc
     6841 taaataaatg agcgtttttt gatagtctat ttcattaggt aatatatatt tgtaacaaat
     6901 caa
//
LOCUS       DBFIFS010000228         6865 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.228, whole genome shotgun sequence.
ACCESSION   DBFIFS010000228 DBFIFS010000000
VERSION     DBFIFS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.228"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            421..1410
                     /gene="araF"
                     /locus_tag="N7A07_004097"
     CDS             421..1410
                     /gene="araF"
                     /locus_tag="N7A07_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU2478654.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1480..2994
                     /gene="araG"
                     /locus_tag="N7A07_004098"
     CDS             1480..2994
                     /gene="araG"
                     /locus_tag="N7A07_004098"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU2478655.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3009..3995
                     /gene="araH"
                     /locus_tag="N7A07_004099"
     CDS             3009..3995
                     /gene="araH"
                     /locus_tag="N7A07_004099"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU2478656.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4162..4962
                     /gene="otsB"
                     /locus_tag="N7A07_004100"
     CDS             4162..4962
                     /gene="otsB"
                     /locus_tag="N7A07_004100"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU2478657.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4937..6361
                     /gene="otsA"
                     /locus_tag="N7A07_004101"
     CDS             4937..6361
                     /gene="otsA"
                     /locus_tag="N7A07_004101"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU2478658.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(6368..6796)
                     /gene="uspC"
                     /locus_tag="N7A07_004102"
     CDS             complement(6368..6796)
                     /gene="uspC"
                     /locus_tag="N7A07_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU2478659.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1718 a         1611 c         1798 g         1738 t
ORIGIN      
        1 taatgacgaa atgaatgacg tgcattttcc acatctttga gttgcggtta ttacaccatt
       61 tcaaaaactc aacgccaggt aatgcggcct attgactggt gaaaaagaag acatcccgca
      121 tgggtaccaa agacaacaag gatttccagg ctaatcttat ggattaatct gctgtgcatt
      181 caacaatttg tctgacaaat tggcttttcc ttatgtcttt tcccgctaaa tttatgcacg
      241 ttctcactgt aattctgcga tgtgatattg ctctcctatg gagaattaat ttctcgctaa
      301 aactatgtca acacagtcac ttatctttta gttaaaaggt aatgctttgt tttccgatta
      361 atttaacgaa tgtcattcgt ttttgccctg cacaaaacga cactaaagct ggagagaacc
      421 atgcacaaat ttactaaagc cctggcagcc attggtctgg cagccgttat gtcacaatcc
      481 gctatggcgg agaacctgaa gctcggtttc ctggtgaagc aaccggaaga gccgtggttc
      541 cagaccgaat ggaagtttgc cgataaagcc gggaaggatt tagggtttga ggttattaag
      601 attgccgtgc cggatggcga aaaaacattg aacgcgatcg atagcctggc cgccagtggc
      661 gcaaaaggtt tcgttatttg tactccggac ccgaaactcg gctctgccat cgtggcgaaa
      721 gcgcgtggct acgatatgaa agtcattgcc gtggatgacc agtttgttaa cgccaaaggt
      781 aagccaatgg ataccgttcc tctggtgatg atggcggcga ccaaaattgg cgaacgtcag
      841 ggccaggaac tgtataaaga gatgcagaaa cgtggctggg atgtcaaaga aagcgcggtg
      901 atggcgatta cctccaacga actggatacg gctcgtcgcc gtactacggg ttctatggat
      961 gcgctgaaag ctgcaggatt cccggaaaaa caaatttatc aggtacctac caaatctaac
     1021 gacatccccg gggcatttga cgctgccaac tcaatgctgg ttcaacatcc ggaagttaaa
     1081 cattggctga tcgtcggtat gaacgacagc accgtgctgg gcggcgtacg cgcgacggaa
     1141 ggtcagggct ttaaagcggc cgatatcatc ggtatcggta ttaacggtgt ggatgcggtg
     1201 agcgaactgt ctaaggcaca ggcaaccggc ttctacggtt ccctgttgcc aagcccggac
     1261 gtacatggtt ataagtccag cgaaatgctt tacaactggg tagcaaaagg tgttgaaccg
     1321 accaaattca ccgaagttac cgacgtggtg ctgatcacgc gtgacaactt taaagaagaa
     1381 ctggagaaaa aaggtttagg cggtaagtaa tttgccggaa aaattcccct ttgcatgatg
     1441 cagagggggt gtgaacgacc agtgattcac ggagacgtta tgcaacagtc taccccgtat
     1501 ctctcatttc gcggcatcgg taaaacgttt cccggcgtta aggcgctgac ggatattagt
     1561 tttgactgct atgccggtca ggttcatgcg ttgatgggtg aaaatggcgc aggaaaatca
     1621 actctcttaa aaatcctcag cggcaactat gcgccaacca cgggttctgt agtgattaat
     1681 gggcaggaaa tgtccttttc cgacacgacc gcagcactta acgcgggtgt ggcgattatt
     1741 taccaggaac tgcatctcgt gccggaaatg accgtcgcgg aaaacatcta tctcggccag
     1801 ctgccgcata aaggcggcat tgtgaatcgc tcattgctga attatgaggc gggtttacaa
     1861 cttaaacatc ttggtatgga tattgacccg gacacgccgc tgaaatatct ctccattggt
     1921 cagtggcaga tggttgaaat cgccaaggcg ctggcgcgta acgctaaaat tatcgccttt
     1981 gatgagccaa ccagctccct ttctgcccgc gaaatcgaca atcttttccg cgttattcgt
     2041 gaactgcgaa aagaggggcg ggtgatctta tacgtttctc accgtatgga agaaatattt
     2101 gccctcagcg atgccatcac tgtctttaaa gatggacgtt atgtcaaaac ctttaccgat
     2161 atgcagcagg ttgaccacga cgcgctggtg caggcgatgg tcgggcgcga cattggcgat
     2221 atctacggct ggcaaccgcg tagttatggc gaggagcgcc tgcgtcttga tgctgtgaaa
     2281 gcaccaggcg tgcgtacgcc aataagtctg gcggttcgca gtggtgaaat tgttgggctg
     2341 tttggtctgg taggggcggg gcgtagcgaa ttaatgaaag gcatgtttgg cgggacgcaa
     2401 atcaccgccg gtcaggttta tatcgaccaa cagccgatcg atattcgtaa accgagccac
     2461 gccattgccg caggcatgat gctctgcccg gaagatcgca aagcggaagg cattattcct
     2521 gtgcactccg ttcgcgacaa tatcaacatc agtgccagac gtaaacatgt gctcggcggt
     2581 tgtgtaatca acaacggttg ggaagaaaac aatgccgatc accacattcg ttcgctcaac
     2641 atcaaaacgc cgggcgctga gcaactgatt atgaatctct caggtggaaa tcagcaaaaa
     2701 gccattctgg gccgctggtt atcggaagag atgaaggtca ttttgctgga tgaacctacg
     2761 cgcggcattg atgttggtgc taagcacgaa atatataacg tgatttatgc gctggcggcg
     2821 cagggtgtgg cggtgctgtt tgcctccagc gacttacctg aagtcctcgg cgttgccgac
     2881 cggattgtgg tgatgcgcga aggtgaaatc gccggtgaat tgttacacga gcaggcagat
     2941 gagcgtcagg cactgagcct tgcgatgcct aaagtcagcc aggcagttgc ctgagtaagg
     3001 agagtatgat gtcttctgtt tctacatcgg gttctggcgc acctaagtcg tcattcagct
     3061 tcgggcgtat ctgggatcag tacggcatgc tggtggtgtt tgcggtgctt tttatcgcct
     3121 gtgccatttt tgtcccgaac tttgccacct ttattaatat gaaagggttg ggcctggcaa
     3181 tttccatgtc ggggatggtg gcgtgtggca tgttgttctg ccttgcttcc ggtgactttg
     3241 acctttctgt cgcctccgta attgcctgtg cgggtgtcac cacggcggtg gttatcaatc
     3301 tgactgaaag cctgtggatt ggcgtggcag cggggttgct gcttggcatt ctctgtggcc
     3361 tggtcaatgg ctttgttatc gccaaactga aaataaatgc tctgatcacg acactggcaa
     3421 cgatgcagat tgttcgaggt ctggcgtaca tcatttccga cggtaaagcg gtcggtatcg
     3481 aagatgaaag cttctttgcc cttggttacg ccaactggtt cggtctgcct gcgccaatct
     3541 ggctcaccgt cgcgtgtctg attatctttg gtttgctgct gaataaaact acctttggtc
     3601 gtaataccct ggcgattggc gggaacgaag aggctgcgcg tctggcgggt gtaccggttg
     3661 ctcgcaccaa aattattatc tttgttctct ctggcctggt atctgcgata gccggaatta
     3721 ttctggcttc acgtatgacc agtgggcagc caatgacgtc gattggttat gagcttattg
     3781 ttatctccgc ctgcgtttta ggtggcgttt ctctgaaagg tggcatcgga aaaatctcat
     3841 atgtggtggc gggtatctta attttaggca ccgtggaaaa cgccatgaac ctgcttaata
     3901 tttctccttt cgcgcagtac gtggttcgcg gcttaatcct gctggcagcg gtgatcttcg
     3961 accgttacaa gcaaaaagcg aaacgcactg tctgatgctt ttttccacaa caatttaacg
     4021 ttttttccca ccacagccag ccgccacaac ggttggctgt tcttcgttgc aaatggcgac
     4081 ccccgtcaca ctgtctatac ttacatgtct gtaaagcgcg ttctgcgcaa cacaataaga
     4141 aaagagaagg aggagaaccg ggtgacagaa ccgttaaccg aaacccctga actatccgcg
     4201 aaatatgcct ggttttttga tctcgatgga acgctggcgg aaatcaaacc gcatcccgat
     4261 caggtcgtcg tacctgacaa tattctgcaa ggacttcagc tactggcaac cgcaagtgat
     4321 ggtgcattgg cattgatatc agggcgctca atggtggagc tggacgcact ggcaaaacct
     4381 tatcgcttcc cgttagcggg cgtgcatggg gcggagcgcc gtgacatcaa tggtaaaacg
     4441 catatcgttc atctgccgga tgcgattgca cgtgatatta acgtgcaact gcatacggtc
     4501 atcgcccagt atcccggtgc ggagctggag acgaaaggga tggcttttgc gctgcattat
     4561 cgtcaggctc cgcagcatga agacgcatta atgacattag cgcaacgtat tactcagatc
     4621 tggccacaaa tggcgttaca gcagggaaag tgtgttgtcg agatcaaacc gagaggtacc
     4681 agtaaaggtg aggcgattgc tgcctttatg caggaagctc cctttatcgg gcgaacgccc
     4741 gtatttctgg gcgatgattt aaccgatgaa tctggcttcg cagtcgttaa ccgactgggc
     4801 ggaatgtcag taaaaattgg cacaggcgca actcaggcat catggcgact ggcgggcgta
     4861 ccggatgtct ggagctggct tgaaatgata accaccgcat tacaacaaaa aagagaaaat
     4921 aacaggagtg atgactatga gtcgtttagt cgtagtatct aaccggattg caccaccaga
     4981 tgagcacgcc gccagtgccg gtggccttgc cgttggcata ctgggggcac tgaaagccgc
     5041 aggcggactg tggtttggct ggagtggtga aacagggaat gaggatcagc cgctaaaaaa
     5101 ggtgaaaaag ggtaacatta cgtgggcctc ttttaacctc agcgaacagg accttgacga
     5161 atactacaac caattctcca atgccgttct ctggcctgct tttcattatc gcctcgatct
     5221 ggtgcaattt cagcgtcctg cctgggacgg ctatctacgc gtaaatgcgt tgctggcaga
     5281 taaattactg ccgctgttgc aagacgatga cattatctgg atccacgatt atcacctgtt
     5341 gccatttgcg catgaattac gcaaacgggg agtgaataat cgcattggtt tctttctgca
     5401 tattcctttc ccgacaccgg aaatcttcaa cgcgctgccg acatatgaca ccttgcttga
     5461 acagctttgt gattatgatt tgctgggttt ccagacagaa aacgatcgtc tggcgttcct
     5521 ggattgcctt tctaacctga cccgcgtcac gacacgtagc gcaaaaagcc atacagcctg
     5581 gggcaaagcg tttcgaacag aagtttaccc gattggcatt gagccaaaag aaatagccaa
     5641 acaggctgcc gggccactgc cgccaaaact ggcgcaactt aaagcggaac tgaaaaacgt
     5701 acaaaatatc ttttctgtcg aacggctgga ttattccaaa ggtttgccag agcgttttct
     5761 cgcctatgaa gcgttgctgg aaaaatatcc gcagcatcat ggtaaaattc gttataccca
     5821 gattgcacca acgtcgcgtg gtgatgtgca agcctatcag gatattcgtc atcagctcga
     5881 aaatgaagcc ggacgaatta atggtaaata cgggcaatta ggctggacgc cgctttatta
     5941 tttgaatcag cattttgacc gtaaattact gatgaaaata ttccgctact ctgacgtggg
     6001 cttagtgacg ccactgcgtg acgggatgaa cctggtagca aaagagtatg ttgccgctca
     6061 ggacccagcc aacccgggcg ttcttgttct ttcgcaattt gcgggagcgg caaacgagtt
     6121 aacgtcggcg ttaattgtta atccctacga tcgtgacgaa gttgcagctg cgctggatcg
     6181 tgcattgact atgtcgctgg cggaacgtat ttcccgtcat gcagaaatgc tggacgttat
     6241 cgtgaaaaac gatattaacc actggcagga gtgcttcatt agcgacctaa agcagatagt
     6301 tccgcgaagc gcggaaagcc agcagcgcga taaagttgct acctttccta agcttgtgta
     6361 ggagcggtta atctcccgta agtggaacta acagcacatc gacctcactt gaggcaatca
     6421 ctcttttcgc cgagcaggat gctcgcgaaa agaaactgtg attgtgatta ccgcaaatca
     6481 ccaaatcgaa atgatatttg cgacaaactt ccagaatatg ttcgcttaac tcaccgtagg
     6541 caataaacgt cttgtcaacg ggataccccg catcctgaat taacttatca agaaagcttt
     6601 gcgtttcttc ctgcatcacg ctgcgtaaat cttcaagcat cggcgcagct aattgattgt
     6661 acatttccgg gtcggaagcg agagtaatta aactgatgtg tccctttact ggcctggcga
     6721 tagataccgc ttttgccagc agttgctgac tttccggtgt aaccgcaaca gcgacaagaa
     6781 tattgctata gctcataacc tgttccttat tctgtgaact tcaggtgaca ttaaagcttt
     6841 actcctaatg gagaaacgac gcaat
//
LOCUS       DBFIFS010000229         6862 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.229, whole genome shotgun sequence.
ACCESSION   DBFIFS010000229 DBFIFS010000000
VERSION     DBFIFS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.229"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(547..864)
                     /gene="yehK"
                     /locus_tag="N7A07_004103"
     CDS             complement(547..864)
                     /gene="yehK"
                     /locus_tag="N7A07_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU2478660.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(926..4555)
                     /gene="yehI"
                     /locus_tag="N7A07_004104"
     CDS             complement(926..4555)
                     /gene="yehI"
                     /locus_tag="N7A07_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU2478661.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            complement(4565..6862)
                     /locus_tag="N7A07_004105"
                     /pseudo
     CDS             complement(4565..6862)
                     /locus_tag="N7A07_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1662 a         1667 c         1697 g         1836 t
ORIGIN      
        1 aatatctgga aagtagatcg cagaaaataa aaaaacctgc tggtgaggca ggttctttta
       61 tttactacag tgacgctaac ttgctgattg tgcaacattt cgtaccaacg aataaattat
      121 aaccatatcc acactccatt gcaattcagt gagtgagatt tataatcctg cccgttggga
      181 ggttgtgccg gatcggacca attttaagtc ctggctgccg gacgggtggt gccgcaggcg
      241 gtgccctgat ccggagagca acgtttcgta ccaacatacg taaaccagta tcgacaggca
      301 gtgtaagggt aaggtgctgg tgttgcgctc cttaataagg agagcggatg gacacgctga
      361 cacaaaagtt aaccgtgctc attgccgtac tggagttatt agtggctctg ttacggttga
      421 ttgatttgtt gaagtaacgg gcaaattgaa acgcatctaa gggggaatac cgtttccccc
      481 tttaagaaag ctatactcgt catacttcaa gttgcatgtg ctgcgacttt aattatctag
      541 agtatattat tctatttctt ggttgaatat attctgtagt tcacgattaa aaattgctat
      601 tacagtttca ggtgattcac ctcgtaattt ccgctcatca atacactcat ctcttatata
      661 aatcaacgca tcatttatta gcgggtcagc atcttctcga tcacgttgat gaaatgttga
      721 acgtagccgg aatcgctcca gtcgatagac ggaacctgta aataattcag agcatttatg
      781 gatctcaagt ctgaaggaaa acggatcttc aggtacaggg acctcataat cgtaaatagc
      841 aaccagctct ttttgcacga tcatcgcttt ccccgctggt aagtcgtgtt gataacgggc
      901 agttgcatca ctgcccgctg tgtttttaat ggaaaatgac gtcaggtgcg cttaacgcct
      961 cactcaaatt aaacacatca agttcggcaa aagtgacgga tttattgtca cggtacactc
     1021 gaagagaacg gaagctcatt aacgctttgc tatcaacatt gtagtggtta aaaccgggtg
     1081 aaaacttaag gaccagccgg tactcaccca ggtcatagac aaatgcgtcc tcataacctg
     1141 actcccagcc tttgtatcgc attcccataa gttgcccaac ggtagcggat ttaccttccc
     1201 agcgagtcag gctgttactg gttgactcat caggtgtgag cagcaccgtg cggcgcgcca
     1261 actggcgaaa aggcggcata atttcgtaat cggcaaaaag ctgtgcaaat tcactgcgca
     1321 tttctgctgt catttctaac gggtgggcaa tgccaatcag agcgtccgca ggcaggtcaa
     1381 ttggctcatc ttgcgcattg cagaaccccc cctctgcggc cacacgaaag gcgttgagta
     1441 aacgacgcgg ttcatttgcc ggataaaccc cccatattaa tcgctgggta accagacggg
     1501 taaagggatg attaacaaac agagaatgaa aatctgccag cgaccagcgt cgggtggtac
     1561 gtagggcagc ttcaagacgc gggagcaggt ttttgctcac ctgagtagca tcttttttta
     1621 gcccttttag tcgggccagt gcctcgggcg ctttcagttg atcgtcatcg gcgcgcaacc
     1681 ggggaacgct tttctggcga acgttttgct gatcaaagat aaccgggttg aggttttcat
     1741 cgaagcgaac ggtaaactgc cggggaccaa aatcaaaact caacgcctgc ggatcatcaa
     1801 ggcccagggt tgggactaat ctgtccgcta gctcttcctg gcttagcccg cgattttctg
     1861 cgactacctg aagaaattcc gccgcgttat cacgtaaggg gcgggattta gcccgttgcg
     1921 atatatgatg cagctgtatc agcgccatat cattattcat cagggtaagg atgttcaggc
     1981 cgctgacagc acgggcagat ttgccttgtg gccaggcgag gatctgtgtt gttagatccc
     2041 gtgccgtgct ttcgtcacca aagagactta acgccagaaa tgcccagttg tcttttgcgg
     2101 gggcaccagc ttgttgccac gcagtgaaga gatcccaggc aaaagctgcc agcgtttgtg
     2161 gctggcagaa cgttttcagt tgttccagcc cgctataaaa acgtcccccc tgggtaaagc
     2221 gcagcatttc gccgataatt tccagagcgt catcggtaac aggttgatta ttagtgataa
     2281 ggcgcggcct ggaccacatt tgcggatgcc agaaatcagg ggcttttgga atgcgtgccg
     2341 ggtaaatttc catgggaccc tgtttcagta aatgctccag ggcaggccat acatctgtac
     2401 gctgccaacg attggctaca gtttgtagca attcgccatg cccatgttcg taaagtaaac
     2461 gcagggcaag taatgcagct tcgcttttat cgctagattt ggtgaagaca agagggataa
     2521 gtgcagtagc cgtatgttcc ggccattgta aaatccactg gcgagccaga tcacgctgcg
     2581 ccgcgaaacg tcgccagacg cgggcaacgg gcagggccag ttctgttgcg ccgaaattta
     2641 aaattaacgg aaatgtttct tttggacgat gtgaaaaagc caacagcaga cctggtagcg
     2701 catccaatcc gaggatactg agaaaatagt cctctcccgc gaacagatgc tttttctcat
     2761 tgatacgcag ccagaaagtc aaagccatct cacgcggcaa ctgtgccaat aaatacaggt
     2821 tccagacact cttttccgga cgataacgtg agtagttgtc attttcccat atgttaatta
     2881 acgcttctat gtgaccgctg cggagcgcct gttctgctaa ttgtgcttcc ggggagttcc
     2941 agccattcag gatctcttca acccgcccct ggtagtaatg ccactgaaca atgagtgaat
     3001 tgcgtgcagc atccagtttt ccagggagta tataattcaa aaacagccat acatcatccc
     3061 caaaacccag ttcctgtaat acaactcgcc cacttttttg ttccatgcgt tgcgcaagag
     3121 attccgtgct gaaataacgg gcagactcag tggcggcaag cctctctcgt tcgcctggtt
     3181 gccagtaaac ttgcggagtt aattcgagcc gtgccaaatc aagacgtgga gctgtcattt
     3241 ttttcttact acgccacggt ggtgaaacga ggatttcagg cagattgctg ttatcggtgc
     3301 catcaataac cggttttggc agtgccgata aataactctt cagcactgcc ctggcttgag
     3361 tacttaacca cgacatcact tctgctgcca gttgtggctg cgatgccatc agcgtttgta
     3421 acagcgtacg ccagtattct tctcgagctt tttgctgtgc aggcagcttt ttgtcttcga
     3481 tgattggata accagggcgg gctggtggtt cttttaacgc cagcagttct gccagtgcgg
     3541 cgagcgtcgc atgggggaag tttttactgt atttagccac acgttgcagg ctgggtttgt
     3601 ttttatcagc aactaacagc aaggtgcgga tgacttgcgg atggttaatt tgtaccagca
     3661 gactgccgca atcttcttta tgagcataca ttgccaggcg cggaacggct gccagtcctt
     3721 gttcgcgcaa aagggaggcg caggccgcgt agccgaagcg gttttcttgt gacatgtagc
     3781 tggcttcgcg atcgctaaat acgtccaggc cccagtatcg ttcgagtttt tttaccgccg
     3841 tattatcagt agcaacgacc tttaaccact cctttgaatg gagacttgac cgggagcttt
     3901 caagactggc gagttcattg gctatttcag gtttttccgg gaggatcagc gcaataaaag
     3961 gacggcgaat tttgggtatt cctggcagtg cggcaatgag tttatcggca caacgctgcc
     4021 agcaactttc ctctgccagc gacagatgct tacgcaaacg gagatcgaaa tcgttatagg
     4081 tcgcggagcc ataaccgtag ccatagtcct cgtcctgata ctgcaaagtg acgaccagtg
     4141 agtcactctc ataacgccgc gcaatgcact gacgcgatat cactacatcc gtcgcatatt
     4201 ccagcccctc ttgttgcacg atttcatcaa gtaactgctg gctgtcattt tgcgcgatac
     4261 agatcagagc cgccatggta agggctggga ttgacggttt atgctcgcca gtcaaattga
     4321 ttgcctgctg gatggcggac tgccactcca gtgagcattt atcataattc caggtgatat
     4381 tttccttctg gcctgcttgt tgtagtaatt catctcccaa tgtctggatg agacttccag
     4441 tgataacgga cactggttcg cccggccagc gacgatgact gagcggcgtt ttgccttttt
     4501 tatatttcag ttccagttgg gcgttatccg ccagccacgg taattccttg tccatgctct
     4561 ttccttattc aaacagggct tcaatatcgt taattagctc gctggcggtg atggcatcga
     4621 gtacggagaa ctgcgcttcc tgtgttgaat tactccccca gccatagctt tctgcgttgc
     4681 cttcccatag ccacagcttg cttaagattt gctcggcact cagttcggct ggcgacatgc
     4741 ctaccgcaaa accttcatcg atttccatga ttaacgacca gcggcccaga ggtttgatca
     4801 tccagccgat ccagcctcca tcctgcgggg tgccctttat ccagccttta ttcgccagcc
     4861 ccatgacccg accgctcggg cattttctgc ctgcccagcg ggtcagttcg ctggcgttgc
     4921 gctcggcttc tgtcagggtg taactgttac ggtcaagctg acggaacggt ggtagcagtt
     4981 catagtcggc aaaaagctga ccaaaggcgg tagcatccgt cggtgatatt tccagaacgt
     5041 gaggaatgcc aacagagata tcgccttccg gcagggtgaa aagatcatcg tcagcggtgc
     5101 tgtagctgtt atcttccgcc acgcggaagc acgtcagtag ctggttttcg gtgctataaa
     5161 cgccccaaat cagacggcgg gttaagtggc gtaccagtgg atgttcaacc aggaagagct
     5221 gaaagttttc tggcgaccag cggcgacgca ggcacatggc ggattccaac cttgctacct
     5281 gctgggcggc gacggtacgc gcatcttttt tcagcagttt gtagcggtta acagcatcgt
     5341 tcgcctgtga ttcatcatcg cttttgtttg gtttgggcag gtctttcaga cggctgccag
     5401 aaacatcacg cacaaacggt tttaaggttt catcaaagct gacggtgaac tggcgtgagc
     5461 cgaaatccag cagcaacgaa ccgttatcat ccagaccgag atccggtgct aaccgatctt
     5521 caagctctgc caccgtgagt tcgcggcttt cggcaatgcc ggcaattttt tcttttgccc
     5581 gctcctgtaa tgctttgaat ttcagtttct gggcaatacc gttaagctgc atcagggcga
     5641 tatcactacc gatagcggcg agaatatcca acccaacggt ggcgcgttta tgctgggatt
     5701 caccaggcca ggcgcgtatt aatggcgtca gtttgcgggc ggtgtcatcg ttaccaagaa
     5761 cgcctaacgc agtgaacgcc caactctctt tcgacggtgc gcccgcagtc agccaggcgg
     5821 taaacagatc ccaggcaaat tcagccagtg agtcggcggt acaggcatct ttcacctgca
     5881 ataatcccgg atacagggcc tcttcctgag ggaagcggag catttcaccg aggtgctgga
     5941 gtgcgctatc tggcagtacc ttgctgctat ttttgagttt cgggcgagtc cataatggag
     6001 gttgataaaa caacggtaaa ggggggattt tcgccgggta tttacccaca ggactgttgt
     6061 cgttattttt attttgagtt tcccagggcg gcgagactag taccattggt agaatttctg
     6121 agtttgcaaa atcttcattg cgactattca gcaattgctg acattgttgc aataaatttg
     6181 ccgcttgcga cgataatccg atgactaatt tttcgacaaa taaaggattt gcattaagta
     6241 atgaaaccag caatgtatgt ttgcgtttat catctttttt taccaatgct tcggctaatg
     6301 ccactatcgt tgcagaagga aaattttcgg cactttttat tcgtaatggc caacggatga
     6361 ttaatacatt tcagtacgtc accgcaatta tcttcttcag catagggagt caggcgagcc
     6421 agtgccgcta taccttgctc ccgtaatacc gtagctgacc aactggcacc ataggtgtta
     6481 aatacatcaa tgtattgata gttctccagt atttcgagcg tgaatggatc tgtcgctacc
     6541 agtttcaaca tttcaagttg tggaacatct gcgacctggt gtaaacgatg tacgatctca
     6601 tgggccactt cgggtatttc tggcattaaa atggcaatta acggttggcg atagagtgat
     6661 atatctggta acgcaacgag taattttgca atgcaatttt gccatacttc ttcttctgct
     6721 agtgataaat gtttacgcag acgtaaacta aatttgtcgt tattccttct ataactgtcc
     6781 ttgctgctat ctgcataatt aataaagaaa gtcaaatgtt catctttata gtcaaaatcg
     6841 aaatcaattt gccaccagaa ga
//
LOCUS       DBFIFS010000230         6841 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.230, whole genome shotgun sequence.
ACCESSION   DBFIFS010000230 DBFIFS010000000
VERSION     DBFIFS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.230"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            206..397
                     /locus_tag="N7A07_004106"
     CDS             206..397
                     /locus_tag="N7A07_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGU2478662.1"
                     /translation="MNKQQQTALNMARFIKSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFSDDGENL"
     gene            1055..1168
                     /locus_tag="N7A07_004107"
     CDS             1055..1168
                     /locus_tag="N7A07_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU2478663.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLLPLAAATGN"
     gene            complement(1566..4202)
                     /locus_tag="N7A07_004108"
     CDS             complement(1566..4202)
                     /locus_tag="N7A07_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU2478664.1"
                     /translation="MAKKPKSVGSEKLLFNRLKQFDEPGLFHDNYQTPDYIQENLKDA
                     LRPYQHGALRYLHYTQRKRDDALLHYRHLLFHMATGAGKTMVMAGTILYLFKELGYQN
                     FIFFVHTDAIIQKTRENLLNPQSPKYLFSQELEIDGEKITIEPIETFPSIPERNTIYL
                     KLSTIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEE
                     QTWERTIENILNLRPDNRLFEFTATIDLANKDIGQKYRGKVVYQYDLKQFMSDGYSKK
                     VMLLEANQNDSDKMLDAVLLSQYRKLTAADHGITGFKPVILFKSNKIAISKAKQEEFS
                     QLIAAMTPESVRRHLANKKLQLSSDTSIWHKVIQRYANSDLVTVTGQIQEDFNDFNLL
                     NVNKSDLLEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKT
                     STDSEAQLIGRGARYYPFIYDGQRSFTRRFDNSTKDLSVLEQLHYHTINEPAYIKTLH
                     ASLEQADIDVHQDGGGTIEHARLKEDFKKSAVYQAGKLYFNEVEEIESSSRNWETYSL
                     ETRFEIPYQTVCEESLDNLTGTKTGITKPELLVLDERFYRKAMQRISFYALDNLQRFF
                     PKLTGIREFIRSDAYLGKLKITVIVPQSLDFTTVPAKDKLHLLETVLLRISENVRRND
                     QKVKGTYRFISQPVKEVIKDYSLHIDPSVVINQKITAAPTIGKKWYVYDNAILNQLEH
                     RLIKTLEAFMPKLKARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNT
                     YWQVFLEPKGDDRLLDDAWKERMLETLNDRERIVIDENEHVRLVGIKFFANSQMDVFV
                     SDMQNKLNDGESLETSSLSLPL"
     gene            complement(4207..6189)
                     /locus_tag="N7A07_004109"
     CDS             complement(4207..6189)
                     /locus_tag="N7A07_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HGU2478665.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKA
                     LVDNEKVRSVYGADIDGIFIFDFDKLCGLLKYKEYWANSFTKYRNKVGLTSEGKYLDY
                     SSDVVLDFPFKDCVLEGGMSKEDQGKSEVYYNEIIARDEIDNLFSPKVLKEVNRYSKE
                     GVKKERIFNRNDNLIIRGNNLVALHCIKNIYEKSVKLIYIDPPYNTDTDSFKYNDKFS
                     HSTWLTFMKNRLEVAKDLLCDDGLIAISIDDNELNYLASLADDIFGRKNRIAIFPRIT
                     KKGGKSASIFAKNHDYILLYVRSLENKRMYAVSHDDDNFKYKDEYFAERGSYKLNQTL
                     DYDSLQYSKSLDYIIEINGEKYYPGGDENLFNDRMNGKHNRADWAWRWSRKLFDFGFE
                     NGFIEIRNGGQRPRIYTKTYQNAKISKDGNDYVITYFDRTKPLSTLEFTENKYSNDNA
                     TKHISSLLGKNIFDYTKPETLLAKLIEITSTKNDLILDFNLGSGTTAAVAHKMGRRYI
                     GIEQMEYINDITVPRLKKVIEGEQGGISKDVNWQGGGSFVYAELMELNAYFVHEIQKA
                     QSTEELEKLFTMMKTEAHLNYQVELENVLSAEYEVDGIFRKVAFSELELHEQKQLLIE
                     ILDKNKLYVNVSDMEDSELSISESDKAFTRSFYGME"
BASE COUNT         1722 a         1639 c         1502 g         1978 t
ORIGIN      
        1 aaaaaaaacc tcgacgcaga gcgacatttt atgcaacagc ggctgtacag cacagggatt
       61 atttatcagt tgatatgctg tgccatgata cctcctaaaa aacaggacag gatgtgcaat
      121 tgtaataccg tcgcgcgcaa cgtcttacaa ttaccacctg gtcagggtcc acccgacacc
      181 cggtactgac ggagaacaac cgaaggtgaa caaacagcaa caaactgcgc tgaatatggc
      241 gcgatttatc aaaagccaga cgctaaccct gctggaaaaa ctcaacgagc tggacgcgga
      301 cgaacaggcg gacatctgcg aatcacttca cgaccacgcc gacgaactct accgcagttg
      361 cctcgctcgc ttcagtgatg atggtgaaaa cctctgacac atacagctcc cggagacggc
      421 cacagcttgc ctgtgagcgg atgccgggag cagacaagcc cttcagggca catcaggggg
      481 tttcggggcg aagccctgaa ccagtcacgt agcgatagcg gagtgtatac tggcttaact
      541 gtgcggaatc agtgcagatt gtaagaaaac agcggcatat gcggtgtgaa atatcgcata
      601 gatgcgtaag gagaacatgc ctgttcaggc actcttccgc ttcctcgctc actgactcgc
      661 tacgctcggc cgttcggctg cggcgagcgg tatcagccca ctcaaaaatg gtaatgcggt
      721 gatccacaga atcaggggat aaagccaaaa ggagcatgtg agcaaaaaac aagaaccagg
      781 aaaaggccgc gccgatggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact taaagatacc aggagtttcc
      901 ccctggtggc tccctcgtgc gctctcctgt tccgaccctg ccgattaccg gatacctgtc
      961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcag
     1021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatt
     1141 gccactggca gcagccactg gtaactgatt tagaggagtt tgtcttgaag ttatgcacct
     1201 gttaaggcta aactgaaaga acagattttg gtgactgcgc tcctccaagc cagttacccg
     1261 gttagataat ctgccaggat tagctaacct tcgaaaaacc acctgccagg gtggtttttt
     1321 cgtttacagg cagcagatta cgcgcagaaa aaaaggatca caagaggatc cttgaatctt
     1381 ttccccctca tccccctagg cataaacata gcgaacggtt tttaaccaca attcaaaact
     1441 aatgattcgc aacagcaaga acaaacaatg caaccattac aggagcaatc atcaactccc
     1501 ggggaggtgg ttagggataa caataaaata acggagcgta atgctccgtt atttattgtc
     1561 gtgatttaca gtggtaggga aagcgaggag gtttcgagcg attctccatc attcagcttg
     1621 ttctgcatgt cgctgacaaa aacgtccatc tggctgttgg caaagaattt aatcccaacc
     1681 agtctgacgt gctcattttc atcaattaca atgcgctccc tatcattcag ggtttcgagc
     1741 atccgttctt tccatgcgtc atccagcaaa cgatcatccc ccttcggttc aaggaacacc
     1801 tgccagtaag tgttgtccga atggcgcgtc agtatcatga tgaaatctgg cataaacccg
     1861 cgcacgccgc cgaactccgt gagcttaaag cgcgttgact gctcgtcgtt acgcagtacg
     1921 taaatatcgt cataacgcgc ttttagcttc ggcatgaatg cttccagcgt tttaattagg
     1981 cgatgctcaa gctgattcag tatcgcatta tcgtacacgt accatttttt accaatggtg
     2041 ggtgcagcag tgattttctg attaataact accgacgggt caatgtgcaa actgtaatct
     2101 ttaataacct ctttcaccgg ctggctgata aagcggtacg tgccttttac cttctgatca
     2161 ttgcggcgaa cgttctctga gatacgcagc agaacggttt ccagtaggtg cagcttatct
     2221 tttgccggta cggtggtgaa atccagtgac tgcggtacga tgactgtaat tttcagctta
     2281 ccaagatagg cgtcactgcg gatgaactca cggatgcctg tcagtttcgg gaagaaacgc
     2341 tgcaggttat caagtgcgta aaagctgatc ctttgcatcg ccttgcggta aaaacgctcg
     2401 tccagtacca gtagttccgg ttttgtgatt ccagtctttg tgcctgtcag gttgtccagc
     2461 gactcctcgc agacggtctg atacggtatc tcaaagcgtg tttccagcga gtaggtttcc
     2521 cagtttctgc tgctgctctc aatctcctcc acttcgttga agtacagctt acccgcctga
     2581 tacacggcgg actttttaaa atcttctttc agtctggcgt gttcaatcgt accgccgcca
     2641 tcctgatgca catcaatgtc cgcctgctcc aaagaggcat gcagtgtttt gatgtacgcc
     2701 ggttcattga tggtgtgata gtgcagttgc tccagtacgc tcaaatcctt cgtactgtta
     2761 tcaaaacggc gggtgaagct tcgttgaccg tcataaatga acgggtagta acgcgcgcct
     2821 cgcccgataa gctgcgcttc gctgtccgtg ctggttttgc tgctgctcgc ctgctcgctg
     2881 atgcggacaa tatcgtagag gttgagaacg tcccagcctt cattcacctt cgcgacggca
     2941 aagacggctc tcaccggatt atcgacttct tctagtgtat tcagcaggac gggattctct
     3001 tccagcagat ctgacttgtt gacgttcagc aggttaaagt cgttaaagtc ctcctgaatt
     3061 tgcccagtca ccgtcactaa atcactgtta gcgtaacgct gaatcacttt atgccagata
     3121 ctggtgtcag agctgagctg gagttttttg ttagctagat gacgtctgac gctctcaggt
     3181 gtcatggcgg ctatcagttg cgaaaactcc tcctgcttcg cctttgatat cgcaatctta
     3241 ttcgacttga acagaatgac cggtttgaag ccggtgatgc cgtgatctgc tgccgtcagt
     3301 ttacggtact ggcttaaaag taccgcatca agcatcttgt cgctgtcatt ctggttggct
     3361 tccagcagca tgactttctt cgagtaaccg tctgacatga actgtttcag gtcatactga
     3421 tacaccacct taccacggta tttctgcccg atatctttgt tcgcaaggtc aatggtggcg
     3481 gtgaactcga acaggcggtt atccggtcgc aggttgagga tgttctcaat cgtgcgttcc
     3541 caggtctgct cctcgttctc cggtgaggtt ttcgacttgc ctctagcttt agtaccggcg
     3601 ttgaaatgat gtgcctcgtc accaagcagc acaaggggga tttcttttaa atcctcgtag
     3661 gtgatactgt tctcacgata gctgttcagc tcgtcgtgca tcttatggat agtagagagc
     3721 ttgaggtaaa tcgtgttacg ttccggtatg gacgggaatg tttctatcgg ttcgatggtg
     3781 attttttccc cgtcgatttc cagctcctga ctgaacaggt acttcggcga ttgcggatta
     3841 agcaagttct ctcgcgtttt ctggataatc gcgtctgtat gcacgaagaa aatgaagttc
     3901 tgatacccta actctttaaa cagataaagt atcgttcctg ccatcaccat cgttttccct
     3961 gcaccggttg ccatgtgaaa cagcaggtgg cgataatgca gcagcgcatc atcccgttta
     4021 cgttgcgtgt aatgcaggta acgcagtgca ccgtgctgat aggggcgcag ggcatctttc
     4081 aggttttcct gaatatagtc cggcgtctga tagttatcgt ggaacagtcc tggttcatcg
     4141 aactgcttca gcctgttaaa cagtagcttc tcactgccga ctgacttcgg tttcttcgcc
     4201 atgccgtcac tccattccgt agaagctgcg ggtaaaggct ttatcgctct ccgaaatgct
     4261 cagttcgctg tcctccatat cagagacatt cacgtagagt ttatttttat cgaggatttc
     4321 aatcagaagc tgcttctgct catgcagttc cagttcactg aaagctacct tacggaagat
     4381 gccatccact tcgtactcag cggacagcac attttccagc tctacctgat agttgagatg
     4441 ggcctctgtt ttcatcatgg taaagagctt ttcgagttct tccgtggact gcgctttctg
     4501 aatttcatgc acgaaatagg cgttcagctc catcaattct gcgtagacaa agctgcctcc
     4561 cccctgccag ttaacgtctt ttgagatccc cccctgttca ccttcgatga cttttttcag
     4621 gcgaggtacc gtaatgtcgt tgatgtactc catctgctca ataccgatat agcgacgccc
     4681 cattttgtga gcaacagcag ctgtagttcc actaccaaga ttaaagtcta gaattaggtc
     4741 atttttagta gatgttattt cgattagctt agccaatagg gtttctggtt ttgtataatc
     4801 aaatatattt ttccctaaga gggagctaat gtgttttgtc gcattgtcgt tagaatactt
     4861 gttttcagta aactcaagtg ttgacaatgg ctttgttcta tcaaagtagg ttatgacata
     4921 atcatttccg tcctttgata tctttgcatt ttgatatgtt tttgtatata tccttggcct
     4981 ttggccgcca tttcttattt caataaaccc attttcaaac ccaaaatcga ataatttcct
     5041 actccatctc caagcccagt ctgccctgtt atgcttgcca ttcattctat cattaaataa
     5101 attttcatca ccacctgggt aatatttctc accattgatt tctattatat aatcaagtga
     5161 tttgctgtat tgaaggctat cataatccaa ggtttggttg agtttgtagc ttcctctctc
     5221 agcaaagtac tcatctttat atttgaaatt atcgtcgtcg tgcgaaactg catacatacg
     5281 cttgttttct aaacttctaa cataaagcag tatgtaatca tgattctttg cgaaaatgct
     5341 ggcggatttc cctcctttct ttgttattct tgggaatata gcaatcctat tttttcggcc
     5401 aaaaatatca tcagccaacg aggccaaata atttaattca ttatcatcaa tactaattgc
     5461 aattaacccg tcatcgcaca acaaatcttt tgcaacttca agtctgttct tcatgaaagt
     5521 cagccacgta gagtgactaa atttatcatt atatttaaag ctatctgtat cagtattata
     5581 tggtggatct atataaatta atttaactga cttctcgtat atatttttta tacagtgcaa
     5641 cgcaactagg ttatttcctc tgataattaa attatcatta cggttaaata ttctttcttt
     5701 ttttactcct tctttagagt aacgatttac ttctttgaga actttcggtg agaacaaatt
     5761 atctatttca tctcgcgcga tgatttcatt atagtaaacc tctgattttc cttgatcttc
     5821 tttcgacata ccaccttcaa gaacacaatc cttgaaagga aaatccagca ccacatcaga
     5881 actgtaatca aggtatttac cttcgctagt taatcccact ttgttccggt atttagtgaa
     5941 gctattagcc caatattctt tgtattttag aagaccacat aatttgtcaa agtcaaagat
     6001 aaaaatgcca tcaatatccg caccataaac agagcggact ttttcattat cgaccaatgc
     6061 cttgataagg tctggctgct tttgactgat agcgtcactc accattgaac gttgtaattc
     6121 ctctccgttc cagaattgcg ggaatgcttt taaaaccttg ttaagttctt ttattaattc
     6181 ggtctgcatg tcatggcctt atgactgtta aatattggaa tgattgtaag gtatagcgtt
     6241 cggtgctgga acccgacagc tatgagttgg atgcagttgc ggggaaatgc atcagaatgg
     6301 gtggtattta acataaacgc tcttgcgcgc accactaaaa cgcgctgaac gcgattctga
     6361 cgcaaacacc gcaaaaagag atgttttacc cagtgaaaac cagaaaaatg cgtcaggatc
     6421 gttttcagct cgatgcatag ctatgcatga aagtgaatga cgatcggttt gagtgcattc
     6481 ggtgccaata aggcacattt tcagacattt tcagttcgtg aattccaggc atatctgagc
     6541 agacacccct aggggcgctg ctaggggtgt cctgtaaaaa aatgcgacgc gcccctaggg
     6601 acgcccatac attaattaac tgttttttat aaagattgtc agtactgacc ctaagtaggg
     6661 tcgccatagg gtcgtcaacg acgtgctttt gcataaatat gaaaaagaaa atacattttt
     6721 gaaaccataa caccatgatt tcaaaagcaa atcagcaaaa aattagtttt gcgtggggtg
     6781 tgggcatttc aggcatgaga acagactcgc gtttttccgg ggtatctgcg gaatttttga
     6841 t
//
LOCUS       DBFIFS010000231         6724 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.231, whole genome shotgun sequence.
ACCESSION   DBFIFS010000231 DBFIFS010000000
VERSION     DBFIFS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.231"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..105)
                     /locus_tag="N7A07_004110"
     CDS             complement(<1..105)
                     /locus_tag="N7A07_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478666.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEV"
     gene            434..1306
                     /locus_tag="N7A07_004111"
     CDS             434..1306
                     /locus_tag="N7A07_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU2478667.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1691..4540
                     /gene="cah"
                     /locus_tag="N7A07_004112"
     CDS             1691..4540
                     /gene="cah"
                     /locus_tag="N7A07_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGU2478668.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4651..>6724
                     /locus_tag="N7A07_004113"
     CDS             4651..>6724
                     /locus_tag="N7A07_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU2478669.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSR"
BASE COUNT         1691 a         1697 c         1902 g         1434 t
ORIGIN      
        1 aacttctccc ggacacgggc gttcacgata atagtcatcg tcatagtttt catcgtcaga
       61 taatgatatg tcgtattctg aatatgattc accacaatta tacatgaaat catctccttt
      121 ggggggggca ataattagtg agtttattcg aaaaaatgat gagcgccaga ccaatccacc
      181 caacttttga ttataatcat gaagatactt gtctcagtac aaattgaatc aaactcaatg
      241 cagcatgatt cacctttata ttctctcagg gaaaccaaat accactactc aactattaag
      301 aacaaaattc gggatgtatg attatcagaa gatacctgca tcctaaataa tttcagacag
      361 atattatcgt tgtgaatccc ccgccacctt tccggtgcgc ggggttttgt cttttttcac
      421 cgggaataca tgtatgaatc cgtctgatgc cattgaggca attgaaaaac cgctctcctc
      481 cctgccttac tcgctttccc gtcacatcct ggaacatctg cgcaaactca ccagtcacga
      541 acccgtgatt ggcattatgg gtaaaagcgg ggccggtaaa tcctcactct gtaatgcact
      601 gtttcagggg gaggtcaccc cggtcagtga tgttcacgcc ggcacccggg aagtgcggcg
      661 cttccgtctg agtggccatg gtcacagcat ggttatcact gacctgcccg gggtgggcga
      721 gagccgggac agggatgcag agtatgaagc cctgtaccgt gacattctgc ctgaactgga
      781 cttggtactg tggctgatta aagccgatga ccgtgccctg tctgtggatg agtatttctg
      841 gcgacacata ctgcaccggg gacatcagca ggtgctgttt gtggtgacgc aggccgacaa
      901 aacagagccc tgccatgaat gggatatggc aggtattcag ccttctcccg cacaggaaca
      961 gaacattcgc gaaaaaacag atgcggtatt ccggctgttc cggcccgtac atccggttgt
     1021 tgccgtatcg gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc tcatgacagc
     1081 gcttcccgac catgccgcca gtcccctgat gacccgcctg caggacgagc tgtgcacgga
     1141 atctgtctgg gggcaggccc gtgaacagtt taccggtgcg gtggaccgga tatttgacac
     1201 ggtggagagc gtctgtgttg cctctgttgc acgcacagtt ctgcgtgccg tccgtgacac
     1261 ggtggtctct gttgcccgcg cggtatggaa ctggattttc ttctgaacag tctgccaggg
     1321 gaaatgtctg attacattgc gttaaccaac tgaaaacatg atacaaacac ctcatgcccc
     1381 tcctgtttct cttgcgtgac tgctctactg ttaatagaat aaaacgatcg ataaaacagg
     1441 tatcgatcgt ttatatcgat cgtttatatc gatcgtttat agtggttaat ggcctgcgaa
     1501 aaggcagctg aatctcttca tcatgcagaa cggaattgca cacaacagac tgatacttct
     1561 ctgtctgatg aagggggaga gcgtttacgc ccgggacgta tcgctgtgcc cgataactct
     1621 gttttgtacc tgccggtatc cacttttgtg ggtaccggct tttttattca ccctctgtaa
     1681 ggaaaagctg atgaaacgac atctgaacac cagctacagg ctggtatgga atcacattac
     1741 gggcaccctg gtggtggcct ccgaactggc ccgctcacgg ggaaaacgcg ccggtgtggc
     1801 ggttgcgctg tctcttgctg ctgtcacatc agtcccggca ctggctgctg acaaggttgt
     1861 acaggcggga gaaaccgtga acgatggaac actgacaaat catgacaacc agattgtctt
     1921 cggtacggcc aacggaatga ccatcagtac cgggctggaa ctggggccgg acagtgaaga
     1981 aaacaccggt gggcaatgga tacagaatgg cgggatagcc ggaaacacca ctgtcaccac
     2041 aaatggtcgt caggtcgtgc tggagggggg aacagccagt gatacggtta ttcgtgacgg
     2101 cgggggacag agcctgaacg gactggcggt gaacaccaca ctgaataaca gaggcgagca
     2161 gtgggtgcat gagggcgggg ttgccaccgg tacaattatc aaccgcgacg gttaccagag
     2221 cgttaaaagt ggcgggctgg caacaggaac catcatcaac accggcgcag aaggcggccc
     2281 tgattctgac aactcgtata cgggtcagaa ggtccaggga acagcagaat ccaccaccat
     2341 caacaaaaat ggacggcaga ttatcttatt ttccgggcta gcccgtgaca ctctcattta
     2401 cgcaggtggt gaccagtcgg tacacggaag ggccctgaat accacactga atggcggtta
     2461 ccaatatgtg cacagggacg gacttgcgct gaacacggta attaacgagg ggggctggca
     2521 ggttgttaag gcaggtggcg ctgccggtaa caccaccata aatcagaacg gtgaactgag
     2581 ggtacatgcc ggcggggaag ccactgcagt cacccagaac acgggcggtg cactggttac
     2641 cagtactgct gcaactgtca tcggcacaaa ccgtctgggg aatttcacgg tggaaaacgg
     2701 taaggctgac ggtgttgttc tggaatccgg cggtcgtctg gatgtactgg agagccattc
     2761 agcacagaat accctagtgg atgacggcgg taccctggca gtgtctgccg gcggtaaggc
     2821 gacaagtgtc accataacat ccggtggtgc cctgattgca gacagtggtg ccactgttga
     2881 ggggaccaat gccagcggta agttcagtat tgatggcaca tccggtcagg ccagcggcct
     2941 gctgctggaa aatggcggca gctttacggt taatgccggg ggacaggctg gcaacaccac
     3001 tgtcggacat cgtggaacac tgacgctggc tgccggggga agtctgagtg gcagaacaca
     3061 gctcagtaaa ggcgccagta tggtactgaa tggtgatgtg gtcagtaccg gcgatattgt
     3121 taacgcaggg gagattcgct ttgataatca gacgacaccg aatgccgcgc tgagccgtgc
     3181 tgttgcaaaa agtaactccc cggtaacgtt ccataaactg accaccacga acctcaccgg
     3241 ccagggcggc accatcaata tgcgtgttcg ccttgatggc agcaatgcct ctgaccagct
     3301 ggtgattaat ggtggtcagg caaccggcaa aacctggctt gcgtttacaa atgtcggaaa
     3361 cagcaacctc ggggtggcaa ccaccggaca gggtatccgg gttgtggatg cacagaatgg
     3421 cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg cttcaggccg gcgcctttaa
     3481 ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg cgcagtgaaa atgcttatcg
     3541 tgctgaagtc cccctgtata catccatgtt gacacaggca atggactatg accggattct
     3601 ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa aataacagcg tccgtctcag
     3661 cattcagggc ggtcatctcg gtcacgataa caacggcggt attgcccgtg gagccacgcc
     3721 ggaaagcagc ggcagctatg gcttcgtccg tctggagggt gacctgctca gaacagaggt
     3781 tgccggtatg tctctgacga caggggtgta tggtgctgca ggccattctt ccgttgatgt
     3841 taaggatgat gacggttccc gcgccggcac ggtccgggat gatgccggca gtctgggcgg
     3901 atacctgaat ctggtacaca catcctccgg cctgtgggct gacattgtgg cccagggaac
     3961 ccgtcacagc atgaaagcgt catcggacaa taacgacttc cgcgcccggg gctggggctg
     4021 gctgggctca ctggaaaccg gtctgccctt cagtatcact gacaatctga tgctggagcc
     4081 acaactgcag tacacctggc agggactctc cctggatgac ggccaggata acgccggtta
     4141 tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc ggtttccgtc tgggcagcca
     4201 caacgatatg acctttggtg aaggcacctc atcccgtgac accctgcgcg acagtgcaaa
     4261 acacagtgtg agtgaactgc cggtgaactg gtgggtacag ccttctgtta tccgcacctt
     4321 cagctcccgg ggtgacatga gcatggggac agccgcagcc ggcagtaaca tgacgttctc
     4381 accgtcccgg aatggcacgt cactggacct gcaggccgga ctggaagccc gtatccggga
     4441 aaatatcacc ctgggcgttc aggccggtta tgcccacagc gtcagcggca gcagcgctga
     4501 aggctataac ggtcaggcta cgctgaatat gactttctga ttatacggta tcccggaaat
     4561 acgatttcag gaaaattcat gcagtaatgg gaaaggacgc tcattgctgt tcaccgcagt
     4621 tctgaattat tgaatttata aaggtgaatg atgcacgaaa agaacatcgc cctgctttgt
     4681 gatgaagccg accggctttt gcaactgaat atcaatctgc tccggcaaat ggttgatgag
     4741 ccggatgtgt tatctgacag taagaacgaa aacgggctgc tttttgataa acggaaagcg
     4801 ctgaaaagaa ttgaggagct ggagggcgaa caaatcaaaa ccgcccgcag ggagatggtg
     4861 ctggctgtgg tcggcacgat gaaggcgggc aaatcaacca ccatcaacgc cattgtgggg
     4921 caggaaattc tgcccaaccg taaccgcccc atgacctcgg taccgacatt gatccgccac
     4981 gttcccggaa aaacggaacc ggtcctccat ctggaacata ttcagcccgt ccgcaattta
     5041 ttaatcacac tgcaggaaaa actcgccacc ccggcagggc agcaagtcgc acagtccctg
     5101 cagcaaaccg gggatacccg cgaactactg gatattctgg cggatgatgt ctggcttaaa
     5161 aatgaatacc acggggaaga cgaaatcttt accggactgg catcgttaaa tgacctggtt
     5221 cgtcttgctg cggcaatggg gactgaattt ccttttgacg aatacgcaga agtgcagaaa
     5281 ctaccggtga tcgacgtggc gttcagccac ctggtgggaa tggatgcatg tcagggaaca
     5341 ctcacactgc tggatacccc cggccctaat gaagccggac aaccacagat ggaaatgatg
     5401 atgcgggatc aactacagaa agcctctgcg gtgctggcgg tgatggatta cacccagatg
     5461 aactcgaaag ccgatgagga ggttcgtaaa gagcttaatg ccattgctga cgtctcagcc
     5521 ggtcgtctgt ttgtgctggt caataaattc gatgaaaaag atcgcaatgg tgatggtgcg
     5581 gatgccgtac gccagaaggt gccagcaatg ctgaacagcg atgtgctgcc cgcctcccgc
     5641 gtttatcccg gctcatcacg tcaggcgtac ctggctaacc gtgcgcttca tgagttacga
     5701 aaaaacggtg cgcttccggt tgatgaagcc tgggtcgatg attttatcag ggaagctttc
     5761 ggacctatgg tggaagagga cgactggaaa gacagcacta aggtaaataa aaaggcagaa
     5821 aaactctgga atatatctct gatcgatcaa ctgataacgg aagtcataca gagctcccat
     5881 tccagagcgg cggcactggc ggttgactct gccgccgcaa aactgatgca gaatgcagaa
     5941 aatgtcagcg aatatctgtc attacgtcat caggggctgc agcaaagcat tcagtcacta
     6001 caggcgcata ttaccagcct gctggcggat atccaggaga ttgaggaatg tcagaatcag
     6061 gtgaccggtg atgtcagaat ggccatggag gacatcaata ccaaaaccgg ggaattactg
     6121 acgaaggtct gtgcctcact ggaggaggag ctgaacgact atttcagaag cggtaaacgc
     6181 aaagaacagc aaatgctgga ggaagaaaat tcagcgcagc cccgggagag aaacgcattc
     6241 gctttctttc acgatatttt cggaaccgga aatcagcatg acaggatgcg ggactttgat
     6301 ccggacagcc ccgaaataaa attcagtgat cgcagggcag cccttgaact gatgacgcaa
     6361 atcgaatcga ccgtgaccag cctgcaccgt gaggctgaag cacagttccg gcctgagctg
     6421 gagaaaatcg tcagcgggat tgaaacaggt tttcgtggca cggccctgta cgccacagaa
     6481 aacattgccg gtcgcatcaa tgcccgcctg gaggatgagg gctttaccgt aaaaatcagt
     6541 tttccggcag tcagccagtt acagacccgg ctcgcggtaa aaataaatct gagtgcgctt
     6601 atggaggaaa gaacggagac cgtcacccgt cgccgtcggc agagtggcgt atggggaacc
     6661 gtttgtcgat ggtttggcac cagtgacctg ggctgggaaa actatgacga ggatgtgagt
     6721 cgca
//
LOCUS       DBFIFS010000232         6698 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.232, whole genome shotgun sequence.
ACCESSION   DBFIFS010000232 DBFIFS010000000
VERSION     DBFIFS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.232"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1082
                     /locus_tag="N7A07_004114"
     CDS             <1..1082
                     /locus_tag="N7A07_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU2478670.1"
                     /translation="IYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSL
                     ALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHT
                     ATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNY
                     TIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLL
                     SRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGEV
                     FGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISL
                     VMTEADKALREAKSEGGNKVIIHHI"
     gene            1263..2753
                     /gene="dgcJ"
                     /locus_tag="N7A07_004115"
     CDS             1263..2753
                     /gene="dgcJ"
                     /locus_tag="N7A07_004115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU2478671.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2796..3299
                     /gene="yeaK"
                     /locus_tag="N7A07_004116"
     CDS             2796..3299
                     /gene="yeaK"
                     /locus_tag="N7A07_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU2478672.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3300..3404)
                     /gene="yoaI"
                     /locus_tag="N7A07_004117"
     CDS             complement(3300..3404)
                     /gene="yoaI"
                     /locus_tag="N7A07_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU2478673.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3574..4020
                     /locus_tag="N7A07_004118"
     CDS             3574..4020
                     /locus_tag="N7A07_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU2478674.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(3977..4798)
                     /gene="nimR"
                     /locus_tag="N7A07_004119"
     CDS             complement(3977..4798)
                     /gene="nimR"
                     /locus_tag="N7A07_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU2478675.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            4895..6076
                     /gene="nimT"
                     /locus_tag="N7A07_004120"
     CDS             4895..6076
                     /gene="nimT"
                     /locus_tag="N7A07_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU2478676.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6131..6478
                     /locus_tag="N7A07_004121"
     CDS             6131..6478
                     /locus_tag="N7A07_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU2478677.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6500..>6698)
                     /gene="yoaF"
                     /locus_tag="N7A07_004122"
     CDS             complement(6500..>6698)
                     /gene="yoaF"
                     /locus_tag="N7A07_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU2478678.1"
                     /translation="PSQPEAPKPPQIGMANPASVYCQQKGGTLIPVQTAQGVSNNCKL
                     PGGETIDEWALWRLDHPAGEK"
BASE COUNT         1622 a         1613 c         1602 g         1861 t
ORIGIN      
        1 ttatctattt agttgaaacc attgtaatta tccataaacc aattaacggc agtacactga
       61 tccagacaaa gtcgaatgat gtttctattt tctatatttt ccgccaactc agttttattt
      121 gtttaacctc gctggcgctc ttttgttatg gaaaagacaa catccttgac aacaataaga
      181 aaaaaactgg cattctgttg ctggcgctga tccctttttt agtttttccc cttctggcac
      241 acaatctgag cagttataac gctgactatt ctttgtatgt cgtcgattac tgtccggaca
      301 atcatactgc gacctgggga atcaactata caaaaatatt ggtttgtctg tgggcatttt
      361 tactgttctt tattatcatg cgtacacgat tagccagcga actatggccg ttaatagcat
      421 tattatgtct ggcatcgcta tgctgcaact tacttctact gactctggat gagtataatt
      481 ataccatctg gtatatcagt cgcgggattg aagtctccag taaactgttt gttgtgtctt
      541 ttctgattta taacattttt caggagctgc aactctccag caaactggca gttcatgatg
      601 tgctgaccaa tatttataat cggcgctact ttttcaacag cgtagagtca ttattgtcgc
      661 gacctgttgt taaggacttc tgtgtcatgc tggttgatat taatcagttc aaacgcatca
      721 atgcccaatg gggacatcgt gtgggtgata aagtgctggt ttcaattgtc gatattatcc
      781 agcaaagcat ccaccccgat gatattttag cgcgactgga gggtgaggtg tttggcttgc
      841 tatttaccga actcaatagt gcccaggcaa aaatcattgc ggaacgtatg cgtaaaaatg
      901 tcgaactcct gaccggcttt agtaacagat atgatgttcc tgaacaaatg accatcagta
      961 ttggcacagt tttttcaacg ggtgacacgc gtaatatctc gcttgtcatg acggaagcag
     1021 ataaagcctt acgcgaagcg aaaagcgagg ggggcaacaa agtgattatt catcatattt
     1081 aagtgcaaaa atattcagag ccatgctttt tgcgtggctt ttgcatacaa tttattacta
     1141 ttacccctaa atttcccctc cccagcggcg cagagatgag tataattagc gcccctgtgc
     1201 caggccgcaa tcgaacttta tctggttttc tcgtttcact aaccgaagga gtgccattta
     1261 tcatgaaatt gcaccataga atgctccggc attttatcgc cgcaagtgtc attgtgctga
     1321 catcttcctt ccttattttt gaacttgtcg ccagcgacag agcaatgagt gcctatctgc
     1381 gctatatcgt gcagaaagca gactcctcct ttctttatga taagtatcag aatcagagta
     1441 ttgccgcgca tgtgatgcgc gctctcgctg ctgagcagtc ggaagtgtcg ccagaacagc
     1501 ggcgcgccat ctgcgaggct tttgagtctg ccaataatac ccatggctta aacctgactg
     1561 cccataaata cccgggctta cgcggcacat tacaaaccgc atccactgac tgcgacacaa
     1621 ttgtggaagc tgcagcacta ttacccgctt ttgatcaggc agtggaaggc aaccgccacc
     1681 aggatgatta cggttcaggt cttgggatgg ccgaagagaa atttcactat tatctcgatc
     1741 tcaatgaccg ctatgtctat ttttatgagc cggttaatgt tgaatacttt gcgatgaata
     1801 actggtccat cctgcagtca ggaagtattg gcatcgatcg caaagatatt gaaaaggtat
     1861 ttaccgggcg taccgtattg tcgagcattt accaggatca gcgtactaaa cagaacgtga
     1921 tgagtttgct gacgccggtg tatgttgcag ggcagctaaa agggattgtg ctgctggata
     1981 ttaacaaaaa caatctgcgg aatatctttt acactcatga ccgccctctc ctctggcgtt
     2041 ttctcaatgt cacgctaacc gatacagatt cggggcgcga cattatcatc aaccagagcg
     2101 aagataatct gttccagtat gtcagttacg tccatgactt accgggcggc attcgtgtct
     2161 cgttatccat tgatattctt tactttatca cgtcttcgtg gaaaagcgtt ctgttctgga
     2221 ttttgacggc gttaattttg ctgaatatgg tgcggatgca cttccgctta taccaaaatg
     2281 tgtcgcgaga aaatattagt gatgcgatga ctggactgta taatcgcaaa attttaaccc
     2341 ctgaactgga gcagcggttg cagaaactgg tgcaatccgg ttcttcggtg atgtttattg
     2401 caattgacat ggacaagtta aagctaataa atgacaccct cggtcatcag gaaggggatt
     2461 tagcgattac gttgttagct caggcgatta aacaatcgat tcgtaaaagt gattatgcca
     2521 tccgactcgg tggcgatgaa ttctgcatca ttcttgtcga ttcgacgccg caaattgcag
     2581 cacaactgcc tgaacgtatc gaaaaacgtc tgcaacatat cgcgccgcag aaagagatcg
     2641 gcttctcttc cggtatttac gcgatgaaag aaaacgatac gttacatgat gcgtataaag
     2701 cttccgatga gcgtttatat gtcaataagc agaacaaaaa cagccgttca tgataacctt
     2761 ctgtggttgt ttgcttgtaa tctcaggagc gtgaaatgac tgaaatggct aaaggaagcg
     2821 tgacgcatca gcggttaatc gctttattat cacaagaagg tgctgatttc cgcgtcgtta
     2881 cccatgaagc ggtaggtaaa tgtgaagcgg tgtcagaaat tcgtggcacc gctctaggcc
     2941 agggtgctaa ggcactggta tgtaaagtca aaggcaatgg cgtaaatcaa catgttctgg
     3001 cgatcctcgc agccgatcaa caggccgatc tgagccaact cgccagccat ataggcggat
     3061 tacgcgcttc gttggccagc ccggcggaag tcgatgaact cacaggctgt gtcttcggcg
     3121 cgatcccccc tttcagcttc catccaaaac tcaaactggt tgccgaccct ctactcttcg
     3181 aacgatttga tgaaatcgcc ttcaatgcag ggatgctgga taaatccgtt attttgaaaa
     3241 ccgcggatta tttgcgcatt gcgcaaccag aactggtcaa tttccgccgc actgcgtaac
     3301 tagcctgtcc gttcaataag cagaacggac aaaaccagca caacagcaat ggcaaaaaac
     3361 gatgacgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcata cccccgggag
     3421 atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac agttcgcctg
     3481 attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaag cgtaaagtaa
     3541 gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt gctgatcctg
     3601 ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt ttcaattctg
     3661 gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat tgaaaaacag
     3721 gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat cgccagcggg
     3781 acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct ggtggcaatt
     3841 gcagtaggcg ttattgtctc ctggctgggt gggcgcggcg tgacgttaat gggcagccag
     3901 ccgcaactgg ttgctgggct gctggtgggc actgttttag gcgtagcgct gtttcgcggc
     3961 gtaccggttg gcccgcttat tgccacgggt ctggtttcgc tgattgtggg gaaacagtag
     4021 ttaatcccgc aatatatcgg cctggcgttt gccccaggcc tttcttaaac atagtaataa
     4081 aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta tcgcctttca
     4141 ctaatccctg aagcgccata atcagctgta actgctgtcg ccactggcga aaactcaacc
     4201 ccgtctcttt tactatcagt cgggcaagat tgcgttcact catcgcaaaa aagccagccc
     4261 attgccccaa tgccccccac tcgacgggct ctttcgccat catctccacc atcgcgcgga
     4321 ttttaggatg agaagaaacg ggtaggtgca gctgttgttg tggctgctga gggagttcat
     4381 caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc gtcgtcctgt
     4441 ttgccagagt taaaattaat tcccggcata agggcgatat tttcaatgtg taacatgttg
     4501 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     4561 cttgattgct atgttccacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     4621 acaaagcatt ttccaccgta caggtaattg caccatgtag cgccagtatc agctgtcctt
     4681 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     4741 ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg tgcatcattt
     4801 tgtccgaatt tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     4861 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     4921 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccactacat tacgcgtcac
     4981 cttcaccggc gcagcaccgt tactcgatac gattcgttcc gcttactcgc tgacgactgc
     5041 gcaaaccggc ttattgacca ccctgccatt attggccttt gcgctaatct cacctctggc
     5101 tgccccggtg gctcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     5161 ctgtgcaggt atcgcaattc gctctctccc ttcgccttac ttattatttg gcggtacagc
     5221 ggtcattggc ggtgggattg cattaggtaa tgtcttactg ccaggattaa ttaaacgcga
     5281 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcggc
     5341 ggctctggga tcggctatgg tagtgccgct ggctttgaac ggttttggct ggcaaggcgc
     5401 gttgctcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     5461 aagccaacaa catgcaaatt tgagtacttc acgcgcctta catactcggg gtatctggcg
     5521 ttcgccgctt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     5581 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     5641 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     5701 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     5761 gggcgcggtt gggctctgct tcatgccagc gcacgcgatc acctggactc tgcttttcgg
     5821 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtctgc gggctagttc
     5881 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgttggcagc
     5941 ctgtgggccg ccgctgatgg gtaaaataca cgatgctaac ggtgactggt ccgtaccact
     6001 aatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgtg ccgggagaga
     6061 caaagaaatt cgctaatatc cggtactata gtgacgtgac aaatcatgcg tgaaagggag
     6121 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggcagaac agagcgatgg
     6181 ttatcgcgta ctggtcgacc gcctctggcc acgcggtatc aaaaaaaccg atttagccct
     6241 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     6301 agtcgtcgat tttgcaacct ttcgcgagca atatcttgca gaactggcac aacacgagca
     6361 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctactcagc
     6421 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     6481 ttagtacagc atccggcggt tatttttcac cagccggatg gtccagtcgc cacaatgccc
     6541 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     6601 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     6661 caatctgcgg cggtttaggt gcttctggct gagaaggg
//
LOCUS       DBFIFS010000233         6592 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.233, whole genome shotgun sequence.
ACCESSION   DBFIFS010000233 DBFIFS010000000
VERSION     DBFIFS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.233"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            464..697
                     /locus_tag="N7A07_004123"
     CDS             464..697
                     /locus_tag="N7A07_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478679.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(675..1082)
                     /locus_tag="N7A07_004124"
     CDS             complement(675..1082)
                     /locus_tag="N7A07_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478680.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(1105..1323)
                     /gene="ydfC"
                     /locus_tag="N7A07_004125"
     CDS             complement(1105..1323)
                     /gene="ydfC"
                     /locus_tag="N7A07_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU2478681.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1396..1695)
                     /locus_tag="N7A07_004126"
     CDS             complement(1396..1695)
                     /locus_tag="N7A07_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478682.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(1959..2366)
                     /locus_tag="N7A07_004127"
     CDS             complement(1959..2366)
                     /locus_tag="N7A07_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478683.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2443..2670
                     /locus_tag="N7A07_004128"
     CDS             2443..2670
                     /locus_tag="N7A07_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2478684.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            2654..3205
                     /locus_tag="N7A07_004129"
     CDS             2654..3205
                     /locus_tag="N7A07_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2478685.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            3177..4217
                     /locus_tag="N7A07_004130"
     CDS             3177..4217
                     /locus_tag="N7A07_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2478686.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            4210..4671
                     /locus_tag="N7A07_004131"
     CDS             4210..4671
                     /locus_tag="N7A07_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2478687.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            4759..5439
                     /locus_tag="N7A07_004132"
     CDS             4759..5439
                     /locus_tag="N7A07_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2478688.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            5436..5600
                     /locus_tag="N7A07_004133"
     CDS             5436..5600
                     /locus_tag="N7A07_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478689.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            5605..6138
                     /locus_tag="N7A07_004134"
                     /pseudo
     CDS             5605..6138
                     /locus_tag="N7A07_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6299..>6592
                     /gene="paa"
                     /locus_tag="N7A07_004135"
     CDS             6299..>6592
                     /gene="paa"
                     /locus_tag="N7A07_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU2478690.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLR"
BASE COUNT         1805 a         1440 c         1680 g         1667 t
ORIGIN      
        1 cccataacac tgtttttagc tttaactgtt ccgcgtgcgc ttagccgcat tcactgcatc
       61 acaaaattca ctttaaaaag ggcggacatc agttcatggg caaacagatg ccgccaaacg
      121 tcaccagaaa attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca
      181 acctgacaac ccggtgtcct caacggggaa ggaataaccc cgccatactt accgctgcgc
      241 catttcgcgg agtgccacaa ccggaagcgc acggtcgacg aaaatttaac gacaggctat
      301 ctatgaacca gcaacctcgc cgtgcgcttt cgcgttatgc tctgactttt cagagaaata
      361 tcctttcagt aaactgtcag tgccggatgt tcacccgtgt ccggcgcacg cactccactt
      421 cacccgtgga gaactcctta attaccaacc ctcaggaggg tgaatgttaa aatcaactct
      481 tattgctaaa tgcctttatc aaaatcgcat ggtaagcagc atttcaatag gcgagtctgc
      541 agttaaaagt attttcgaag agtactttcc cgggcatgat tttaataaat ggaataccaa
      601 attaccgcca gcagtttcaa cgcgtattct gaaagcaacc gaaagagcaa gtacaattcg
      661 cgttaactat ttcattaaag atttgtggga tctttgatat ccacagagcc taaagtatgt
      721 gcatatggat gtgctattat gcgccctcgc agatttgcat cattttctaa attcactgaa
      781 cgaaacaggg catcaacaag gctctgtaca atgcaaaggc aatcgaagac tgtcgccgtt
      841 tctgttttga ttgatgaaag aacatggcca ttcacgcaaa cagaaattac ccgtttatta
      901 acatcgcttt cctgcttttg attatcagaa ccatatagcc cagaaaaagc attgcgcaca
      961 ttacgaacca tattatcgat ggtttctttt tctgcggtac taaggtcaag agtagccagt
     1021 tgtgaacgaa ctatattcga tgccatttcc tgtaatggcg ttggtaaatc tttaaattcc
     1081 attattagcc tcgttggtta gctattaacg tgggtatgta atcattctgg caatgcttaa
     1141 tgccgctgct ttttccagat tggtgatatc ctgctccaga gcggacagat tttcagcctg
     1201 cttagccctg gcttcattgg cccatttcag gtcctgcacc gccttaattt tctggtgcat
     1261 ccactcataa agttcatcat cggtatagtc tggcgcgatg atgacggggt ctcgtttctg
     1321 catgtcggct ccttgtggtt agcgttgcct gctttttaac cacgtcaggc gaggtggtat
     1381 cctctgaggg gtctgttact cgagaggaaa ttggttatga atacaatcaa gttttcttgc
     1441 ccagaatgtg gtggctaagt ctttgacaca tcctttaagc cgcagggctc tgacagtttc
     1501 gcgggagcca tctgcaaaaa ttgtggtcac cttgtaactg aagatgagtc ctcgcagttc
     1561 gatgacgaaa tcgttgacaa tatcttcggt gcactcacca gagactttct gaagtaaagg
     1621 cgcataccgc ttagttaccg ctctgataac tcttacctgt ccggcaatgg cgctgatatc
     1681 aatataaagc gccatcgctg tttctttgct gatccctgga cgccttccat tctgatgttt
     1741 gactcgccca ctgagaaatc ctctgcttcc ccttaacgcc gggtagcgga actgtttgct
     1801 gagaacaccg tgcggtgtct tgatgagtag aatttagaat agcctaagag ttatggtcaa
     1861 gctttttatg tagaaaaacc taagcttctt gatgtaaaaa acacaagtat ttgaaagttt
     1921 gtgcttttta ttacagagag ttgcgaaaaa aaggggggtt atttatttgc gcttcttttg
     1981 cgagctttga gtagttcttc aaaaagtttg ttgaaattct caactcgagc acgcatctct
     2041 gacaacagag ccttttgctc tgactcaggc agtgcgtcga acagttgaag caactctttt
     2101 tgatcttctg tcagattgac tggctgatta tctgggatcg gttcgcctgg ttgcttatct
     2161 tcatctccaa aaagaagcca agtcggcgag cactgaagcg cctggctcag tgcgaataat
     2221 ctcttccccg ctggctgtgt ttcatctctt tcccattgag aaattgttac gtgagccact
     2281 ttgaccagct tacctaatgc ggcctgagac agttttaatt tttttcgcct gtataagagg
     2341 cgagcaccga aggtttcgtt tttcatatta ggtaattcta atttttcttg acttaggttt
     2401 ctctacgatc tagtttcctt aggaaaatct aagggtttcg atatgttgaa aattgatgct
     2461 atagcgtttt ttggcagcaa aacaaagctt gccaatgtcg caggagttag gctggcaagc
     2521 gttgctgcat ggggggaact ggttcctgaa ggtcgcgcga tgcgcctgca agaggcatcc
     2581 ggcggggaac ttcagtacga ccccaaagtt tatgacgaat atcgtaaggc aaagcgggcg
     2641 gggcggttga acaatgaaaa tcaccactga acaggtttgt gaggctctgg atacctgggt
     2701 atgccgacca ggaatgacac aggagcaggc gacgatatta atcacggaag cattctgggc
     2761 tctgaaagaa cgcccgaaca tcgatgttca acgcgtcacg tttaatgatg gcgaggttga
     2821 tcaacgggcg ctgggcgtta accgggtgaa gatattcgaa cgctggaaag ctatcgacac
     2881 cagagataag cgtgacaaat tcacggcgct gattccggca attatggagg ctatccggat
     2941 cagcgatttc agattgtatt gtgaaattac tgacggaaaa agcattacgt acatgatcgc
     3001 cgggttaaac aaagaatatg gcgatgtggt ggagtccggg ctgctttttg cggatccagt
     3061 tgttgtggaa cgtgagactg acgagcttat agaaaaagct attgctttca agcacgcgta
     3121 tcgtcagcaa taccaatatt actttgcaga taaacaaatg tctgccaggg gggcgtatga
     3181 gtatcgatgc actacgatgg gctaaaaagg tgaaaaccgg cagttcatcc agtaagtcag
     3241 tattgacctg gcttgctgat atgtgcggtg ccgatttgtg tgcatacccg tctgtatctg
     3301 cactggcaga agtaacggaa ctgaacaaaa agactgtgca ggacagctta cgacacctga
     3361 tggagattgg gttaattgtt gataccggtg agagaaaagg cagaacaaag caaattgtgg
     3421 tgtaccgact tatcggtgta gaagaaagtg ttgccgagcc tgaatacacc caaaaacggg
     3481 agtctttaaa ggtgggtaaa attggtgctg ttaataaaaa cagtaccgaa aatggttatg
     3541 tttcagcaca aaacagaccc aaaaacagaa ctcttagctg catggaaaat aaccaaagac
     3601 acccaaattt tccatcaaag acacccaaaa acggatcacg gaacccaaag gaacccaaag
     3661 atctaaaccc cacacataac gcacgcgaga gtgctccgac cagtgagcag gaagttttgt
     3721 cgttacaggc agccccccct gtattcctgt atggcctgag cgaacccatc ggaaaattcc
     3781 cgatgagcga tagctggtat ccgtcacggg attttcgacg acgggctgcg ttgtggggga
     3841 tggctttgcc ggagacagaa tttacacctg ctgaacttgc cgccttccgg gactactggg
     3901 cagcggaggg gaaagtgttt acgcagattc agtgggagca gaaattcgcc cgtcacgtaa
     3961 atcacgtcag ggcgcaggtt aaaccagtca gcaaaggggt aaaccatgca gcagcaccag
     4021 gtggcaccgc atcacgggca gttcaggaaa ttcgggcagc acgtgagcag tgggaacgtg
     4081 aaaacggatt tatcagcgac ggaaacggcc tggaagctgt gggaactcat gggggtggtt
     4141 tattcgaacc gctggatcca gaagaacggg gccgcacctt cgaagctctg gattgcacag
     4201 attggcgcga tgactgagca gcaaatccgg caggtctgcc gccagtgcat ggaccgctgc
     4261 cgggcgggtg aaacatggcc tccggacctg gctgagtttg tggcactgat ttcgaaaagc
     4321 ggagccaatc cattcggtct gacggtggat gctgtgatgg aggagtaccg ccgctggcgc
     4381 aatgagtcct ggcgatacga cggaagtgat aagtacccgt ggtctcagcc tgtgctgtat
     4441 cacatttgcc tcgagatgcg ttcaaagggg attgagcgcc agatgaccga aggggaatta
     4501 aaacggcttg cagaacggca actgacgaaa tgggcaaagc atgttagtaa cggcctgagc
     4561 gttccgccag tccggcgaca actggcggcc cccaaacgcc cgtcggggcc aacgccaatt
     4621 gagttgctga aacaggaata tgaacgccgg aaagcggctg gttttgtttg agttgagaag
     4681 tgatttttta ccgggaggaa atttatggag actgtttttg acgcactgaa agcgatggga
     4741 aaagccacgt cggtatagct ggctgcgcga cttgatatca gtcgtgaaga agtactgaac
     4801 gagctgtggg aactgaaaaa ggctggcttc gttgataaaa gcgtatacac ctggcgtgtg
     4861 gctgataaca acgttcagca ggaacagcca gcgccagaag aaaccaccac ggcaacagaa
     4921 gcgaaaatct cagagtgcga tttaaccgcg acgattgaac aacgcggacc acaaacggcg
     4981 gatgaactgg ctacgctgtt cggtacaaca tcccgcaaag ttgcttcaac gctggcaatg
     5041 gcaatcagca aaggtcgtct gattcgcgta aaccagaacg gtaaaattcg ttactgcata
     5101 ccaggtgata atttaccagc agagccgaaa gttgaatcgg tagcggaaac cgatggtaaa
     5161 gcctttcctc agccagccgg tgttgcgtta ccggtacaga aagatgcaac acaggaagat
     5221 attaaaacag aaactgtggc ggacattgtg cagtcgctgc catcgtttac tgcaacgcga
     5281 gaagatgatt tgattttgcc atcgctgcat atggcaaatc gcgaactgcg tagggcgaag
     5341 aatcatgtcc agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg
     5401 gatatggttg ccgggatttg tcggaagtcc gggcaatgag cggatggtgc aggcctgaaa
     5461 tcatgatact aacaatgaag gtaaaatgca tcggcagtct gattggtcgt agtgaggcgg
     5521 cggtcaggat gaaagcccag gttaagggaa taagcctgat tctgcggggt gattttcacc
     5581 agtcaacaaa atatccgtag cgcgataacg gtcaaaaatt atggcgctga cacttttgtg
     5641 ccactggaga tgactgtacc taagttcagg ggagaagaac acgtccggtg ggatggtcgg
     5701 gccagattta aagggcaggt catggctcca gcctgtacgc tggcaatgga ggctgcctgg
     5761 cgggaaattg atatgggaac cacgccactc agggatttac tgccggtcca gagaataaat
     5821 tcctgttacg gttacaccac tgtgatcttg caagtgcagg aaagtaggtc tacacggtaa
     5881 cgcgagtgcg tgtaactttt gatgtcattc ccgtagaaac accggacaaa ttttcgctga
     5941 caggtcatgc agaaggtata aatctgcaga ttatggacaa ttacggatat ccggcaagag
     6001 ccggaaaaag catgccgcct ctaattctca gtggaagatg gacttgatta tactcattgc
     6061 attgtcagaa atagttatcc attaaaggct ggctattcca aacaggatgt tgattacaaa
     6121 aatgtaatca acatgtaagg tttatactct tcaatatgcg tataattttc cttattttgt
     6181 tgactttaaa taacaagcta tgcacgaggt aaagtcggat aagtttatct ggatgtaata
     6241 tatattattt gtagtgttta taactttatt tcatgataac caataaaagg agttttttat
     6301 gaggaacata atggcaggtt ttttaatatt cctgtcttct gctgcttatg ctgatatcaa
     6361 tctgtatggt cctggtggcc cgcatacagc cttgcttgat gcagccaaac tttatgccga
     6421 aaaaacaggt attatagtga acgttcatta cggcccacag aacaaatgga atgaagatgc
     6481 caaaaaaaat gcagatatct tgtttggcgc atcagaacaa tctgctctgg ctatcattcg
     6541 ggaccataaa gacagcttca gtgaaaaaga tattcagcct ctttatctgc ga
//
LOCUS       DBFIFS010000234         6555 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.234, whole genome shotgun sequence.
ACCESSION   DBFIFS010000234 DBFIFS010000000
VERSION     DBFIFS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.234"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..2689)
                     /locus_tag="N7A07_004136"
     CDS             complement(<1..2689)
                     /locus_tag="N7A07_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU2478691.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQL"
     gene            complement(2746..5118)
                     /locus_tag="N7A07_004137"
     CDS             complement(2746..5118)
                     /locus_tag="N7A07_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU2478692.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5156..>6555)
                     /locus_tag="N7A07_004138"
     CDS             complement(5156..>6555)
                     /locus_tag="N7A07_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU2478693.1"
                     /translation="KLLTIRWREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRI
                     AEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTV
                     VLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQE
                     LSDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKS
                     RQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADA
                     SIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADS
                     WYVSQTPLIKTGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILS
                     SGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPG
                     VWNLADDICDISAVL"
BASE COUNT         1661 a         1521 c         1494 g         1879 t
ORIGIN      
        1 acaattgctc ctgctcagtc catgctgcag gatcgtcata ttgaataaga ctatttacaa
       61 tagtgttcgc taattgttga acgctacgct gttggatatc gaggctgcgc tgaacgtttt
      121 gctggtattc gttcagttct tgctcactga tccctttagt caaacgctta accatcactt
      181 cattcgctaa cgttaacagt tcatcatgtc gttctggttg acaagtaaaa gccagcaaat
      241 gactgatatc tttagcctga ggatcaaccg agaggcgaga agaaacgctg tatgccccag
      301 atgcctgttc acgaatatta acgcgtagat cttttgccag tgcgacgtta aaagcatcga
      361 gcgccatacg cgtcgccaga ttaacaggtg tccgggaatc ataacgcttc cactgtgaaa
      421 cctgtgccac aggttcattt tgttccttta cagtaaccga tgcgttgtcc gtcgcgcgag
      481 ttaatggttt acctgcggct aatggcgaat cagagtgttt gattgatcct aagtaacgcg
      541 taattaacgc cacgagtttg tcttctgcga cattaccgac aatgacaaac gtgatatccg
      601 ctggagatga aaacaactgg cgatcggcag ccagcgcatc tgcggcagta aactgtgcaa
      661 tctgattttc ttgcagtaat ttcgtgcggt catcagcata gcgcgtctca tacatctgct
      721 gggcgaattt ctccgctgga cgctggtcga gcgttttcaa tgcctgaatt tgagcatttt
      781 gtagcgatgc ccaaatattg tcgttaatcg tgctgtgggt gattcgctgg ttaatcaact
      841 gaaaaccagg ttcagggtta ttagtccgcg cgctaacgga gagcaacgta ttcatgccac
      901 tgactttact gctcatggtt accgagtttt ccgcactcca gcgtttcagg ctggaagagg
      961 agagttcgcc aacgccgctt ccgctaactg ctttatttgc cagcgcgata agtgattttt
     1021 gctgcgcagg gaaacttaaa tcgcctttat tcgatacggc aataatttgt agcttttgct
     1081 cttcacccgc ggattttgcc agaataaccc ttgcaccatt ggaaagtgtt aatgatgtca
     1141 gattctcagc cagcgtttct ttgctgctaa tttccgcctg tgggtcagcg tctactgtta
     1201 acgataaatt tctgcctggg aagatgtaag ccgccagctt tttgttggcg tactcctttt
     1261 ccagcgccag gatagccgca ggagacaatg cttttttggc ggcaacctca ttgtttacca
     1321 tttgctccca aaatgcgtcc tggttctttc ttaactgctg ccatttttcc gccagacttt
     1381 gcacggtaat ttgctgccac agacgtttcg aaagttgata tgtctcttcc ggcgacaaga
     1441 acggcgtatt atttaatgag ctggatgcca ggcgactggt cagcatacgt aaatcacgtt
     1501 cagcttgctg atcaaccgca tttttcagcc aggtgaggcg ggtagatttg acatcatcga
     1561 gttcttcagc agaaaaacca tgctgatcaa tggttgccaa ctcttccatt aatgcattcg
     1621 cagcatcctg catattatcg tctcgtgcat ttacacggaa aaacagcgac tgataatcgg
     1681 gtgcaatttt aacgctgcgc gcagtgccgc cagaaatagt cttcaactcg cccgactgta
     1741 tacgttcctg cagacgttga ttgaacagct gaactaacat gctccattca gcttgttcga
     1801 taaagctttg ctcatcgttc acttgtacca ttggcaggcg ataatagagt gcgatgccgt
     1861 tcacccggtt ttctttatca ttaatgatat taaagcgcag gtggttttcg gcttttgtcg
     1921 gccagacgcg attttcagct gctttgttag ccggaagctt acttaaatta tcctttatca
     1981 gcgccagcgc ttctttactg tcgatatcgc caaccacgat aaaggtcata ttatttggtt
     2041 gataccagcg ttgataaaat tggagcaatt gtgccggtgt gaccgtggcg acagtatcca
     2101 tcaggccgat aggttcacgg tctaaattac gggtatttgc cagcaggaaa gggcggcgcg
     2161 cctgagaggt gcgccatttc gcatcctgat gggcacgcca ttcctcagta attacgccgc
     2221 gttcagcgtc tacttcgagt ttttcaaagg ttgcggcatt actccattca ctgaagattg
     2281 ccatcacttg ttgcagattt tgtttctgcg tagttggcaa actcacctga tacaccgttt
     2341 cgtcatagct ggtataggca ttaacatcgc gaccaaaacg caggcccatt gactcaaatg
     2401 tttcgatgac tttattaccc ggccatgttt ttgtgccgtt aaacatcata tgttctacaa
     2461 aatgagccac gccgcgctca ttgtcttctt cctgcaatga accggtatga atttgcagcc
     2521 ataaatttac ctgatccttt ggatgagcat gcggataaat catatatcgc aagccattgt
     2581 ccagttgccc ggtaattaac ttttcatcct gcggtaaggc ggcggcaatc agccgcccgg
     2641 ggagcaacag agttgccact aacgtcagta agaaacagag gtttctcata attatctcca
     2701 tgcgaaaacc gggcgaattt acccggttaa gtaaaatccg aactattaaa atttcatgct
     2761 gacatcaagc cagaaagtac ggccactggc gtaggtcgcc acaccggtat tcgttgtatc
     2821 aaccgcggtt ttgctatcca gtacattgag aatatcggca ctgatcgtca ggttttgttg
     2881 tttcagaaat tgtggcgtcc aggacaagcg ggtgtcccag gtcaggctgc tatccagttt
     2941 ttcgtcaaca tactgcttgt aatctgaata ttcgctgatg tattgcgcat ttgtcttacc
     3001 caggataatg cgagctttac gcgcttcttg ccaggccagc gtattcgccc acaccaaccc
     3061 gctcggttga tgcgtgaaat ccatgtttaa ggagatcttt aatgggttat taaaatctgc
     3121 cactggaacg ctatcgtaag agacaagatt accgttataa accacctggt tatcaccggt
     3181 attgctctcc tcataaccat tgtttaacga caagttgccc ttgctcttga tatagctaaa
     3241 gacaatttgc gggttaatat ctacctggct gatatgcagg ggttcggcca gttcaaaact
     3301 gaggttgaac gaatgcgttt tggttttgcc gtcgttgtta tattcagtaa tggtggtttt
     3361 agtcgcgctg tcggtacgac tgcttttgct gatttgatca tgcgcttcac ggtaaacata
     3421 gtttgcgcgc gcaataacgt tcttaccgat tttttgctgc aatcccattg ccagttcatc
     3481 gttataaggc gttttcaaat cctgataacg cgtcagggtt ttattacctg ataccgattc
     3541 cgtccagcta ttgcggatat cacgtaatcc catatcaaga atattcccgc catagtaacg
     3601 gttataacct gctgtaatca ttgaggtttg atcagcaaaa atatcccatt ccgtcataaa
     3661 gcgcggggag atattgtggt ttgacagata gttgtcatag tcataccgca cgccgggcat
     3721 taatgacaca ttacgccagc taatgcgatc cgccatatac agtgtgtagt tgtcaattcc
     3781 caggctgcct ttacctttat ggtaaatggt atggttagtt ttctttccgg cagcattaat
     3841 cacataggat tcagactggt tatggcgttc agtccacgca tcggaataga tgtattccgc
     3901 gccgaagtag ggttgatgcg aaacattacc cacggcgaat ttttgccagt ccaggcgtgt
     3961 tttgaaggtg taattatcta cagcctggga aatgtgtcct aatccgccac gggtgcaacg
     4021 ccctgtaata tcaccatatg tacatgaaag ttcggtgtac cagatgtcat gatcgtgacg
     4081 ggtataatca ctaatatgat cccaaccaac ggtggtacgt agtttggccc atgcgagctg
     4141 cgtatccata tcccatgcca taccatagga tttattaccc atttcgcgat cagactgcgg
     4201 gaaggtgctg gtattataat cacggctgga gccggtatat tttaaggtta aatcgtgggt
     4261 aaagcggtcg ctggcaaacc aggtaaattt gctcaatgca gtatcgataa cgtttttata
     4321 ctgtgcccga ccggcgacaa tgccgtcatt cgaaacataa tccgcgcggg tgatatcaga
     4381 ctggcggcgc gataaaccgg cggtaacgcc aaagttatca gcgagttcct gattaaacga
     4441 caaggtataa aagttctttt taaaatctgg ggagtaataa gtacttcctg aagagccttg
     4501 attaaatgcg ctcttgttat tctcattgat atgcgatgtt aaccagtccg aacgcgtagt
     4561 gcgataaccc agtttcacgc tgctatcatc agcgttgaag cgtttgaact ttgcatcaat
     4621 taccccgcca ttgaagcgac cgaattcaac cggcacaaaa ctgtcataaa gcgtcacatt
     4681 gtccagtaag ctgacatcaa gataataccc ctgtgacatc ccgctaatat tggttgcact
     4741 actggcatcg gactcattcg ctgggttcag gttattagtg gcactaatac cgtcaatcaa
     4801 ataggcattc tgatagggcg acgcaccgtg aatagagatt ttctccgggc gaatatctcc
     4861 ctggttcaac gaggtacttt gcgttgaatc catgcgtacc gcagggttgg ttctcagcag
     4921 atcgctgata ttgccattcc cggtcggtaa acgctcgatg ctttcactgg tgtaatgcgt
     4981 gttgccgttt acgggtacag ggacaggagc atagaccgtc atctcttcaa aaaaatgcat
     5041 gtacatgttt ttgtcatcga cggcctgcgc cacatcagcg ccacataaaa tgacgccagg
     5101 aataagaact cgcttcattt atttatatct ccgtacagca gggcttttat tgtttttata
     5161 aaaccgcgct aatgtcacaa atatcatcgg ccaggttcca gacaccgggt tgatgtgtaa
     5221 ccataatgac gccgcttgtg ggtagttttt cacgcactag acgcagtaag cggatagcct
     5281 cttgttcctc aagatgagag gtagtttcgt caagaaatat ccatttcgga cgtcgtaaaa
     5341 ttaatcgtgc cagggcgata cgttgttttt cgccgctgga aagaatatct ccccagcgat
     5401 cgtggtcatg aatacgcgca gccaatttcc caagaccaac ctgatgcagt acttcgctca
     5461 atgatttatc gtctacgggc aggggaagtg ctttacaaat aatctctttc agtaagccgg
     5521 ttttgattaa cggtgtttgt gacacatacc aactgtcggc aggagaagaa atatcacctt
     5581 taaaccacgg ccagcagtgg gataatgttt taagcagtgt ggtttttccc gcaccagagt
     5641 aacctttcag taatagccat ttgcctggcg aaacatgaaa gttcaggttc tctaatatga
     5701 tcttattatc aggagtacga atactcgcgt cagccacttg taccgcatgt tggcaatttt
     5761 taggcttatt cgtagggcgc tgttcggtga gttgatggaa ctcatacaag cgatcgataa
     5821 ccgcagccag ttcagcaagt tcatcatatt tataaataaa ccagcttaga ttgttcgata
     5881 ccagcataaa tgcctggcgc gatttcatca gtccgcccag attgatctgc ccgctaataa
     5941 actgcggcaa taacagaaag tagggaagaa cgctaagcga gcgcgaataa atattctgcc
     6001 agtaatcaag ccaccgctga cgattcatta aacgatgcca gttctcttta atggtatgaa
     6061 aattgtcgct caactcctgg cgttgtagag actcagcatt gcttagcgca atcagttctg
     6121 cctgcttatt atgctgcaca agattagttc gaaatgtcgc ttcgctacgt tgtttttcca
     6181 cattaagcgg acgaatacgt ttacccacct tatgggtaaa taaagttcca ccgatcacaa
     6241 tgagcacaac ggtatagacc atatacccct ggatattcca ttctgttcca ccaacagtga
     6301 atgagagcat acccgcgctt tgccaaagaa taacggtaaa ggtgatcagc atgctgagtg
     6361 actggatgaa gccaaatgaa agactgagcg ttttgctgat cagtaagaga atgtcttcag
     6421 cgatacgttg gtcagggtta tctgtatttt tatgctcgcc gtagatctgc gtgaagtagt
     6481 agtttttatc tgcaaaccag cgattaaggt agtaatctgt taaccattcg cgccagcgaa
     6541 tagtcagtag tttaa
//
LOCUS       DBFIFS010000235         6411 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.235, whole genome shotgun sequence.
ACCESSION   DBFIFS010000235 DBFIFS010000000
VERSION     DBFIFS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.235"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            191..316
                     /locus_tag="N7A07_004139"
                     /pseudo
     CDS             191..316
                     /locus_tag="N7A07_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            381..641
                     /locus_tag="N7A07_004140"
                     /pseudo
     CDS             381..641
                     /locus_tag="N7A07_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            691..930
                     /locus_tag="N7A07_004141"
                     /pseudo
     CDS             691..930
                     /locus_tag="N7A07_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(978..1595)
                     /gene="ureG"
                     /locus_tag="N7A07_004142"
     CDS             complement(978..1595)
                     /gene="ureG"
                     /locus_tag="N7A07_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU2478694.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1607..2281)
                     /locus_tag="N7A07_004143"
     CDS             complement(1607..2281)
                     /locus_tag="N7A07_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGU2478695.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(2282..2746)
                     /gene="ureE"
                     /locus_tag="N7A07_004144"
     CDS             complement(2282..2746)
                     /gene="ureE"
                     /locus_tag="N7A07_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU2478696.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2756..4459)
                     /gene="ureC"
                     /locus_tag="N7A07_004145"
     CDS             complement(2756..4459)
                     /gene="ureC"
                     /locus_tag="N7A07_004145"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU2478697.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(4452..4772)
                     /locus_tag="N7A07_004146"
     CDS             complement(4452..4772)
                     /locus_tag="N7A07_004146"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU2478698.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4781..5083)
                     /locus_tag="N7A07_004147"
     CDS             complement(4781..5083)
                     /locus_tag="N7A07_004147"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU2478699.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(5174..5872)
                     /locus_tag="N7A07_004148"
     CDS             complement(5174..5872)
                     /locus_tag="N7A07_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU2478700.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            6253..>6411
                     /locus_tag="N7A07_004149"
     CDS             6253..>6411
                     /locus_tag="N7A07_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478701.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTG"
BASE COUNT         1496 a         1658 c         1696 g         1561 t
ORIGIN      
        1 gataatataa caacacccat tattttattc tgacctaaaa acttcttatg aaataaacgt
       61 ttacccgacg tatctgatcc cgctgttttt acctgtatcc tttaacatac ttgagaagcc
      121 cgctgaaaaa atataccgta aaggcggttt ctctgtcttc cataaacctg tcggataaga
      181 agggcattaa atgaaaacca aaaaagttat gatgattgca ctggtcagct ctgcgctggc
      241 tctttcagga tgtggagcca tgggcacagc gatcaaaaaa cgaaatctgg aagtaaaaac
      301 ccagatgagt gaaacctgag gtagcctgag tttaacggac actccttcct gaaatagaat
      361 ggcatcagaa ggagctaata atgagcagaa aaacccaacg ttactctaaa gagttcaaag
      421 ccgaagctgt cagaacggtt cttgaaaatc aactttcgat cagtgaaggc gcttcccgat
      481 tatctcttcc tgaaggcact ttaggacaat gggttaccgc cgccagaaaa gggctcggta
      541 ctcctggttc ccgcacggtg gctgaactgg aatctgaaat tctgcaactg cgtaaggcgt
      601 taaatgaagc tcgccttgag cgagatatat taaaaaaagc agctgtagat tcaatctgtc
      661 aatgcaacac ccctttcaat tatctctttc actgcccgcg acggtatcgg ttatcagttt
      721 gacctgctgg ctaacggtga aaccgtactg cgtgatgcat ccggcgaaaa acgcatcaag
      781 ggtgaaaaag acggtcgtat gctcatgaat gttggcctga atgcggaaat tcgcgacaac
      841 atccgcttcg gtctggagtt tgaaaaatcg gcatctggta agtacaacgt ggataacgcg
      901 gtgaacgcca acttccgtta ttccttctga taaaaacaag gggccgcctg ccggccctga
      961 tacagaggat gttgtgatta cttacccagc atgccctgct cttcgataaa ggcgatgatg
     1021 ttttgcagac cttcaccact tttcaggtta gtgaaccccc atgggcgttc tccgcgcata
     1081 cgtaaagtat cacgctccat tacctcgagg gaggcgccca cgtatggtgc aaggtcagtt
     1141 ttattgatca cgaggaagtc ggatttggtg atccccggac cgcctttacg cgggatcttt
     1201 tctccttctg ccacatctat gacatatatg gtaaggtccg ccagttcggg gctgaaggtc
     1261 gcactcaggt tgtcgccgcc gctttccaca aagatcagct cgagattacc aaatttctca
     1321 ctcagtgctt ccacggctgc cagattcatt gatgcgtctt cacgaatggc tgtatgaggg
     1381 cagccccccg tttcgacacc gacaatgcgt tccgcttcca gtgctccggc ctcagtgaga
     1441 atgcgttgat cttctctggt gtagatgtcg ttggtgacta cggctaattg ccaggtgtca
     1501 cgcatggatt tgcagagggc ttccagaagc gcggttttac ctgagccgac cggaccgccg
     1561 acgccgacac gcagcggttg cttgattatg ttcattttat tttctcctaa gaacggaata
     1621 atcgggaata ctgggtttca tgtcgcgcgg aagcgataga tgcaagtggc gttgctgaac
     1681 caattgcgtg atccggggtt gcaagggcac tgtccatgtt cgccgcatag cgggcacaca
     1741 ggcgcataat taactgctgg gcagccagct ggccgtaggg taccagcctg acgcccgcca
     1801 tcactgcgct ttcaatccag ctgtagccaa ggctcagggc caggtctgaa acggatattt
     1861 gccagcgtac gccgagccat gccataccgg ttaactggct ttgctggcat agtttgcgcc
     1921 attcggccgg gcagtcgggc tgccagtcga ccagaagtct ggtgaaggct gcaccgcggt
     1981 tacgttcttc atcgcgtagc tcacgggttt ccctgcaggc cagcagataa gcggtccagc
     2041 gcccggcact gtcaggatcg cccgcttcac aggcccggta tagccgggca agcagtggca
     2101 ggtcgacggc aaaaaaactc tgttccatct gcaactgctg ccagtgttca aaggcggcgc
     2161 tatcttctac ccagcctgct tccactgccc actccagtcc ctgggaccag ctgtagcctc
     2221 caacagggag gctgttactg gcaagctgca tcagccgcag acgtttttcc ggagtcggca
     2281 ttcagtggct gtgctcctga tcgtgattat gggcgtgcga cttgctggcg taagcgccgg
     2341 cttccggctc aaacggaaga tgtgcaaaat caacatccag cccaaactgg cgcagcatat
     2401 catcgaggac gtgatcgtga tggtagcgca gctctccggg cattatttgc agcggaacgt
     2461 ggcgattgcc gagatgccag caggctttcg cgagcataaa aggatctgcg cagcgcacta
     2521 cagaaacggc ttcatcagcg gcgatcacct taatgaattc atcaccgttt tcgttactga
     2581 gaatatcacc atcgcgcagc agcagaccgc gtggcagcaa cagtccagcc tgacgtccat
     2641 cgttaagggt gactttaata cggcttttta cccgcatgtc aacagggagg gtaacgctgg
     2701 cagttgtctg agccggggtc tccacccgcc gggtcaaata aagcatttgt gctccttaaa
     2761 acaggaaata acgttgcgcc attggcagaa tgtccgccgg ttcactggtt atcagttcgc
     2821 cgttgatacg cacttcgtag gtttgtgaat ccacggttat atcaggcaac aggctgttgt
     2881 ggcgcatatc ggctttttgt actgtgcggc agccttttac cacagcagtt gtgctgtgca
     2941 ggttaagctg ttcagcgacg ccatttgctg ctgctgcctg cgacaggaaa gtcacacgac
     3001 agcggtgacg ggcactgccc aatgcagcga acattgggcg atagtgcacc ggctgcggtg
     3061 tggggataga gccgttgata tcacccatcg gcgccatggc tatcattccg cctttgacga
     3121 tagtcgccgg ttttacgcca aagaacgccg gggaccagag caccagatcc gccagttttc
     3181 ccacttcaat cgagccgact tcatgagcaa taccgtgggt taatgccgga ttaatggtgt
     3241 atttagcgat atagcgcttc acgcggacgt tgtcgttatc accactttct tccggtaacg
     3301 ggccgcgctg aactttcatc cggtgtgcca cctgccaggt tcgtaacact acttctccga
     3361 cgcgtcccat ggcctgcgaa tcggacgagg tgagggagaa cgcgccaagg tcgtgcagga
     3421 cgtcttccgc ggcaatggtt tcctggcgaa tgcgcgattc ggcaaaggct acgtcctcgg
     3481 cgatatccgg gtcaagatga tggcaaacca tcagcatgtc cagatgctca tcaatagtgt
     3541 tgacggtata gggtagcgtc ggattggttg aggaaggcag aatattgggg tgcgcgcagg
     3601 cggtgataat atccggagca tgaccaccac ctgcaccttc tgtatggaag gtatggatag
     3661 tgcgcccgcc gatggcagtc agagtatcct caacgaatcc tgattcgtta agcgtatcac
     3721 tgtgtagcgc aacctgaacg tccatttcgt cggctacagt cagtgcgcag ttgattaccg
     3781 caggtgtagc tccccagtct tcgtgaattt tgaggccgat aaccccggcc gcgacctgct
     3841 cacgaagcgc atccggattg gagcaattgc ctttacccag caacccgata ttgaccggca
     3901 ggctgtctgc agcctgcagc atttgataaa tgtaccatgg tcctggggta caggttgtgg
     3961 cgttagaacc cgctgtcggg ccagtaccgc caccgatcat ggtggtaatg ccggatgtca
     4021 gcgcttcttc agcctgctgt gggcagatcc agtgaatatg cgtatcgacg ccacctgcgg
     4081 taacgatcct gccttctgcg gcaataattt ccgtggatac gccgattggg atagtgacgt
     4141 tgggttgtat atcaggatta ccggctttgc cgatagcaaa tatccttcca tctttgacgc
     4201 cgatatcggc tttaacgatc ccccagtaat cgatgatcag ggcattggtc agcaccagat
     4261 cagcgcagcc ggcggagagc atttgcccct gtcccatacc gtcgcggatt actttaccgc
     4321 cgccgaattt gacctcttcg ccgtaggtag ttaaatcatc ttcgacctcg atccacagct
     4381 cagtgtctgc cagacgaatt ttatcaccgg tggtagggcc gaacatgtca gcataggcct
     4441 ggcgtgaaat attactcatc atttgcctcc agctcaccca ttatatctcc gcggaaaccg
     4501 tgcacgatac gggcaccgct cagcgccacc agttcaacct cacgcttttg gcctggttcg
     4561 aagcgcactg cagttccggc ggcgatattg agacgatagc ctcttgcctt ctggcgatcg
     4621 aacttcagtg ccgggttaac ctccgcgaag tgataatggg aaccgacctg aattggtcgg
     4681 tctccgtgat tttcaacgat aatactgcag gtagcccggc cgatgttaag ctctatatag
     4741 ccaggtttaa ccttatattc tccagggatc atctgttacc tcagattatc ggattatgga
     4801 cggtaaccag cttggagccg tccggaaagg tggcttcgac ctggatatcg gggatcattt
     4861 ctggtatgcc ttccatgacc tgctcgcgac tcaggacatg ccgtccttct tccatcagcg
     4921 cagcgacgct tttgccgtcg cgagcgccct ccattataaa agcgctaatc agggccacgg
     4981 attcgggata gttaagtttc aggccgcggg ccagacgacg ctctgccagc agtgcagcgg
     5041 taaacagtaa tagtttgtct ttttctcttg gagtcagttc catagcgttc tcttaagtca
     5101 gccagatccg gggaagtacc ggtgatttgt cagtcagttg cggccgcata aattgccaga
     5161 tatcacgcat tacctactgg caacgcagat tatcatcact taaaaagcga actgtcagta
     5221 gaccatcggt gagcgtggct ccggcgtagt tttccagtgg tgtgagtttt tcgcgtatgt
     5281 cttccagttg cgtttcattc cccaggtaga acagcatcgt tcccacccat ggtttccggg
     5341 caacgcaggt aagttctcca ttggccacgt gcagacgctc aatcaacagc ggagaaccgt
     5401 cgacccatat ctccagacgg ttagcgagtg ttccgtggct gaaggtttct ccgatgaccg
     5461 gacgtccaag gcacagaaga tcccaggcca gcagtgtact ggaggacgac agatgaaaca
     5521 cggtatgcaa cgtggcgtga gcgccgggaa aaaaaatcga atcctgtggc aaccactcca
     5581 gcgttgcgtt attgcacagc gtcaggtttt gctgaaggtg cgcctgcgcg ccgctgctgc
     5641 ggtaaaattt actggcacca ggcatggtga tcagcgtatg gcaatcagag tcaattattg
     5701 cgttgatgat gagttcgtca ccgccgacga taccaccggg tggatgaagc agacagaggt
     5761 ggcaggtttc ttcttcggga taaaaaggac gctgaacagt gagcagaccg acatgatgac
     5821 gggtggtgag cgtggttttc ccgtgcagac gctgaaaacg gagatccagc atggcctgcc
     5881 agcctttttt attttgtggt aacgcggtac tgagcaatct tacgcccctg ataaataaag
     5941 ataaaacgac agtaaagagc cggatttcct tgttatataa aagatgatgc ctttccagtg
     6001 agttttcgtc tgaataatac gaatgtttgt tttcgtcatg aagcgttccg gctcccgggg
     6061 ctatacatcg ccagaaaagc aatgcatatt gtcgggatta tccagagaat caggcagaaa
     6121 gatccgacaa tgggctgtga ttatgccatc tggctcagaa tgatatcaag gtgttatggg
     6181 gataacttgc tgatacaata gcagtatgac agattcatta atgcggaaac tgctcactga
     6241 aacagagtgt tcatgactga gcactggtcg ggtcatgcag atgataattt ccccttttct
     6301 tggggaagcg acagtatttt catgcatgcc tgtcttcagt catacgggga gagcatctgt
     6361 gatacagtcc atacatttgt tgcggacgtc gctcctgagt cactgactgg t
//
LOCUS       DBFIFS010000236         6401 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.236, whole genome shotgun sequence.
ACCESSION   DBFIFS010000236 DBFIFS010000000
VERSION     DBFIFS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.236"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..261)
                     /locus_tag="N7A07_004150"
     CDS             complement(<1..261)
                     /locus_tag="N7A07_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HGU2478702.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDN"
     gene            complement(258..1628)
                     /locus_tag="N7A07_004151"
     CDS             complement(258..1628)
                     /locus_tag="N7A07_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HGU2478703.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(1632..2873)
                     /locus_tag="N7A07_004152"
     CDS             complement(1632..2873)
                     /locus_tag="N7A07_004152"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HGU2478704.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMPMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(2873..4318)
                     /locus_tag="N7A07_004153"
     CDS             complement(2873..4318)
                     /locus_tag="N7A07_004153"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HGU2478705.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(4337..5725)
                     /gene="glmL"
                     /locus_tag="N7A07_004154"
     CDS             complement(4337..5725)
                     /gene="glmL"
                     /locus_tag="N7A07_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HGU2478706.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(5725..6174)
                     /gene="glmS"
                     /locus_tag="N7A07_004155"
     CDS             complement(5725..6174)
                     /gene="glmS"
                     /locus_tag="N7A07_004155"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HGU2478707.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         1446 a         1657 c         1667 g         1631 t
ORIGIN      
        1 attgtcacct acattcccgg agataatatc tcgtggcatg acgattttta ctgcgttaga
       61 aggatcaaac cagacaatac gatgaataaa gtcaggttca acatgatata agcgtgcaat
      121 cagttctggg gttaattgcc ctgatgattt aactcgctca tagttttctt tcgttttaaa
      181 taaaatatct aaaaccagtt cgtaaggtcc ggcatttttc gaacgaatga cttgcgccag
      241 tgagacaatt gactgtttca tgcctgaact ccttctggcg tcacctgttc aatatggaaa
      301 tcaaaacgca gagcgtcgtt ggcttcaatc aggtgatata tggaaaattc gtataccggc
      361 ccgccctgga tatcagaagg ggagaacggg aacgccagat tacctgcggt agcgatgcgg
      421 ttttcatagc cgtagtgcag catggtagag cgcaccagcg agcaaacgct gttggcaatt
      481 tcctgggtcg gtgcaactac atcaaggaca atccccagct catgcccggc agtttgcatc
      541 ggttcatggt ctcccatcac cccgtttttg ccgtacaggt ggaaattgat gcgaatgctg
      601 tcatcgtcga gcgacaggtt acgcgaaacg ctggttttga cttcatcgat gattttatcg
      661 atcccggcga tcataatcgg gtcgcgcgtt ccggcgatgg tcagacagcg gaagcccacc
      721 cgtcgtgcac cttccagttt cagggcatac ggcgtttctt catgcttaga accgctgacg
      781 tagacttcgc cgtcatttac tgctttaaat gtgcagcctt tcaggttcaa cacgccgcca
      841 ggtcctggca ggaagtaggg atcggatttc tcatacagtg tgtgtgcagc tgctgacgtt
      901 tcggtaaatt tacgcttcgg attaaatgtt ttcagcgtaa agccgttgtc atcaatgatg
      961 cccatcgcac agtcagagcc tgagccgggc gttgcggcga ttgccgcgca ttcaaggatt
     1021 ttcccacaat gcagcgccag accttcatca aagccttgca tgatcgggag cgcagcaaag
     1081 caggccgggt cgtaagcgcg cccgcccagc accacttgcg cgcgttcttt cagcgcccgc
     1141 tggaagggtt cgatgcccat ttgcgcgacg atatacgtac tttcgtcaat cgcgtcgtgg
     1201 gttaacggcg ggacaaaatc cagcgccgtg attttgccgt tatccagcgc ctggtggacg
     1261 atggctttgt caacgtctgc cgggatcaac gccatcgaga acgagaggtg ctcttcccgg
     1321 gcaatctcat gaattatttc tcggcaccac tccaggtgtg gtgcggcacc agaaccgcca
     1381 gccgttccga tcaccaccgg tatattattt tgcacgcctg cggtgatcat atagcgcaga
     1441 tcgcgcttga cccctgcgcg atcggtaaac ggtttgcctg ccccgaggta gtgggggcct
     1501 ggatcggaag agcctgcatc aacggcgatc agatcgggcg attctgccat cgctttgcgg
     1561 aagctctctt ccgggaatcc atacccgagg attgccgttg gcgataagac tttaaaagaa
     1621 cgagccattt gttaatcctt agcctgcaac agcgcgatag tacgattcat ttcgttgaag
     1681 acgatatcga ggccttcgtc aaagcccatc cctggtttta ccagcatagg catggggcga
     1741 gcggcaaggg cgacgtggac acaggtgcgg gcactgacat cagtttcatt gcaggtgccg
     1801 ccctggtacg cttccatgct gtggctgttg cagtaaagaa ccgcatcgac gatgttgtga
     1861 atactgccca gatccggggt tttgatttgc accatgtgac aactggcagc atcagtgaaa
     1921 tcaacaatat cctggtaggt gttacaccat tcatcggcca caattttcac gccggaaccg
     1981 aggcgcgtca gctctttggt aatcgcggtc agcaggcgaa tttgatcggg cttgttaccg
     2041 gcatcgaccg gcccttcgat gtagagcggc aggccttgcg cttctttttc caggctggcg
     2101 atgtattgcg cacagcgaag cggatccata tcgaagatca gaccgatagt gccgtataca
     2161 tcgatgtgca gggtagggtg gtagcgtgcg ctggtgcgct tgcttagaat gcgatccgac
     2221 aaccagcgga catattcgcg cagtttttca cctttaaagc ccagcttctc ttcgacgtta
     2281 ttaatcagcg catggggcag cacgtcgatg cctttaagga tcatcttatc gacggcgata
     2341 tatcgatcgt cgccgctctg accaaataat ggaatggatt ccgccacgcg tggcaactgc
     2401 cattcatcac agaccacttc agttttcaga cggccggttg ccagcgcggt agcatcaagc
     2461 agcgcctgtg ataatccgta gcgcacggcg gtatgcagca agttgccgtc aatacgcaat
     2521 ttgtcgaaga aacgggcatt cggcaggaaa gcatccacat cgcggcctac cagtaatggc
     2581 ttgatatggt cgttgaggaa cggaataaag tgctctgcga ggaacagtgg atcgcgaccg
     2641 cctgccccgg aatactgtac ggcagtacag tcacctacgg cgaccgcgcc gttttccaga
     2701 atcaactgta ccgatacgca ctccccggcc tgacgtacgg cgttaaaccc ctgtgttact
     2761 ggctccccgg tataaaaaaa gccgtcatga cccgctccgt tttttatcgc ctgctggtca
     2821 tcgaaataga atgaggagta gccagcggtg aacagagcct gttttatttt cattatggtc
     2881 ttcctattaa tttactgtgg gatacagcat tgatgtcatc tacaaccatc tggaatgtcg
     2941 gttggcgtcc ttcacagcgt gcgcgttccg caacgtaatc gtggtggagg tcgagaatat
     3001 cttttggcaa tggaacggct ccggcttcca gtacgcgtat cgcgccggtg ttgtcacgga
     3061 ccggaagaat tttgcccgcg ttgcaggcgg ctggcgcaaa cggcacatcc agtacgcccg
     3121 cttcaaatgc cagcaccgtt ccgcgcgcaa tatcaccatt acccagttcg aacactttat
     3181 tcagtaccgc gcgaacctcg ctcttaatca gttcaatttc cagttcaacc gcagggcacg
     3241 gcgggaattt ctgctcattg accatgttga gcatttggcg ggaggctttc agcccctgaa
     3301 tgttggcagc ggcagtcggg ataccccagg cttcgtgcgg acttttggtg atgactttgg
     3361 tggcaccaga catccccgcg acggctgcgc cccaggagat aatagcgaac gctttcgatt
     3421 cgtcttccgg aaagccgccc atccactggt gaaacacggt actcaattca tagtcggtat
     3481 agccatagct ctgaaaatat tcgtgtgcaa gttcacgcag agactggatc gccgcaacat
     3541 cctgcgtcag gctgccgacc tggccataac caacggtgat agatttcaca ccctgttcca
     3601 gcgccagcaa gccttcgatt atcgcgatag agtgcgagat aaacggcggg atcagcgtac
     3661 ccgttagtgg gccaaacggt tcgcggttga tgcggatccc gtgttcttca tacatcccca
     3721 ttaaacggtc gcagtactgc cagtcgcgga tcgatttttc cagcgttacg cgtttggcat
     3781 acggaatgtt gtaggaaatg ccaccgcctt cataactggt aaagccgctg gccattgaga
     3841 tttcagccag cagacgcgca tctggcgtgc cgtggcggat ctgtagcggt ttttgcaggg
     3901 tttcggtgag gcgacggcaa gctgcgacac catggttaac taccggtaaa ccgttcagtt
     3961 tagaggttcc ggcttcgatg gattttttaa tccctacagc cgcttcttcg taccgattca
     4021 gacgggtata tgcatcgatg gtgcttggca gcagatcgca ctcttcttgt agtgttttca
     4081 gcagttcgat gtgttcgtcc atcagcgcca cacctgcgcg cggttggcta agggttttac
     4141 cttccttgtc tgctttcagc agcgcaagag agaaacgttt atgctcaggg atggtttgtt
     4201 ggtatttaac gccatcttca aagttttcaa catccttgcc ggtttcccag gttttcagta
     4261 cctgttgccg ctcggtcatg aattcgtcat gggttaattt cttatttcga agttccattg
     4321 attgcagcct taattgttat agggttaagc attgaatact ggtgcgcgcg gcggccagcg
     4381 gatcgactct ggcgacgttt gccagcagtg gtaatagccc ccgggaatcg cggtaatatt
     4441 caaattcggt cggtaagaga atgcgtttac cgtcgtcgtt taactcgcgg tatttcaacc
     4501 agtgatgcat atcgaactga ctggcacgcg agagccatcc gccggatccg acgactttac
     4561 gcacggttgt caggtcacgt cccatctgta aatcgacatt gccgacgcag gtgcagacct
     4621 cttttttggt gcccgcatgt ctttcggtgg cataacccac acacagtccg gccaggacgg
     4681 tatcgaaata tttctcttct gcatttgcgg gtaagtagtc gggatgagcg actagatgac
     4741 ggaggtaatg ataaaaagcg tccagctgtg cgggttgctg agcaaaattc acattgacta
     4801 actctttcgc actttcacca accaccactg ccgagacgcg cattcccaga tcgccttcca
     4861 ccgtccgttt tacgaagggt tccggtacgc cgtgtaaaac ggtgtcgggc gagagcgtgt
     4921 tggcacaggc ggaataaaca tcggtggttg cgccgcccat gtcgatcagc atgaactctt
     4981 tccagctgtg atcgacgtta ctgattgcct tgaccagttc aaagaccgtc catggtgtcg
     5041 gcattggttc ttcgcctgtt ttatcaacaa tgacatccag ccctttccct ttaacgatgc
     5101 gtttaaggaa tatgtcgcaa atagcctggc gtgcagctac agggttcgga tggtcaagat
     5161 cgggcagaac gttgtcgaca atggtcaggt ctttatgccc gaggatctcc tgcacctcgt
     5221 cctgaatatc ccggtttccg gcataaataa tggcgcagtc gagtttggat tctgccagca
     5281 cacgggcgtt attcagtccg tagctttctt ctccgccgtc ggtgccgccg gtgaacaaga
     5341 gaatatcggg ctgcgtttct tccagtgcct ggatatcacg gcggttcagc ttgtaggcgt
     5401 aatattgcgc aattttagca cccgcagagt gagcggttac tttcgcggtt tccagggtga
     5461 tagaaggcac caatcccatt gcggcaaccg ccagcccacc tttggcagag gaggaatact
     5521 tcaatgcaac ctcaccgctg tttaacaggg ggagggcatt atctacgttt aacacctggt
     5581 tcaggctgga gaaaaacccc ttcgccagat gatgggtggt ggttggggtc agtacgtggt
     5641 taaccaacgt taacgcgtcc ccctcccggg taaagagggc tgctttggtc cacgtcgatc
     5701 caatatcgac agagacgatt tgcatcagat agcctcttca agaatacgag tatcaacgtc
     5761 atgacgttgg ttgatatcgt gggccatcag ctggcaaaca tcttctaagt catgacttgg
     5821 ggcaaagacg cgatcaaatc ccatctcttt gaatttggtt tccacgtcag cgaagtcatg
     5881 ttttccgacc accaggttac cgcccacata gagcaggatg tcccccagtc cacgctcgat
     5941 gcagcgttcg cgcatgccca gacagtcgat atcgccatgc ccatagatag aggagacgac
     6001 aatcgcgtca gcgccagttt caatggcagc atcaatatat tcgtcctggc tgaccattac
     6061 gcccagatta atgactctga agtcgtggtt gctaaaaacg cggtccagaa ctttattgcc
     6121 tactgcatgg cagtccgcgc caataacgcc aatcacaagt gttgctttct tcatgggtaa
     6181 tcctttgtaa gacagagagt aaaataagtt acacacgtcg tatttatggc cgttcataac
     6241 ggcacaggtt taatatcaca ggagtgtctt tatctgaccg taatgttgca attgatctat
     6301 tttcaaaatg tctttcggga aatgattgtt tgaagtgtgt ttttaaattt ccatgttaat
     6361 tcagtgtgtt atgtcaacta atgtcatgag tgttaaattt t
//
LOCUS       DBFIFS010000237         6371 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.237, whole genome shotgun sequence.
ACCESSION   DBFIFS010000237 DBFIFS010000000
VERSION     DBFIFS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.237"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..234
                     /locus_tag="N7A07_004156"
     CDS             <1..234
                     /locus_tag="N7A07_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2478708.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..2135
                     /locus_tag="N7A07_004157"
                     /pseudo
     CDS             206..2135
                     /locus_tag="N7A07_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2119..2325
                     /locus_tag="N7A07_004158"
     CDS             2119..2325
                     /locus_tag="N7A07_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU2478709.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2322..3914
                     /locus_tag="N7A07_004159"
     CDS             2322..3914
                     /locus_tag="N7A07_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2478710.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3904..5409
                     /locus_tag="N7A07_004160"
     CDS             3904..5409
                     /locus_tag="N7A07_004160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU2478711.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5446..5793
                     /locus_tag="N7A07_004161"
     CDS             5446..5793
                     /locus_tag="N7A07_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2478712.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5851..6102
                     /locus_tag="N7A07_004162"
                     /pseudo
     CDS             5851..6102
                     /locus_tag="N7A07_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6099..>6371
                     /locus_tag="N7A07_004163"
     CDS             6099..>6371
                     /locus_tag="N7A07_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU2478713.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKG"
BASE COUNT         1454 a         1561 c         2000 g         1356 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg gggggcgggt
      481 attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat tctgccgctg
      541 aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc
      601 tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa cgcttttcat
      661 cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg
      721 ttgacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa aaagagggct
      781 cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa tccattcgcg
      841 gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc
      901 attttatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat ctgaaatttg
      961 gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa agtgttttct
     1021 acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag agcaacgggc
     1081 ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc
     1141 ggggtgatga aattccgccg ccgcgctcca tcatgttcca tatctggacg gcgtacagtc
     1201 cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa gatcccaacg
     1261 gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa
     1321 aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg gtgcctgccc
     1381 gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag atgtatgtct
     1441 ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt atggggcgtc
     1501 ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg
     1561 cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg atcgatggtg
     1621 aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg
     1681 catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag cggggcgtgt
     1741 atctgtgtga agtgggaacg gacaccgcaa aagaaattct ctatgcccgt atgaaagccg
     1801 atccctcgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat gatccggaga
     1861 ttttttcgca gacagaggcg cagcaactgg tggcggaata gctggtggag aagtgggaaa
     1921 aaggaaagat gcgtctgctg tgggataaca aaaagcggcg taacgaagcg ctggactgcc
     1981 tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg ctggcagctt gatctggctg
     2041 tactggcaaa atcccgggaa gaagagacga cccggccaac cctgaaagaa ctggcagcga
     2101 agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg tcaggcgcgc
     2161 cttgatttat taaccggtaa acgggtggtg tctgtccaga aagatggtcg cagaattgaa
     2221 tatacggcgg cttctctgga tgagcttaac cgggcgatca atgatgcgga gtcggtactg
     2281 gggacaaccc ggcgtcgccg tcgtccgctg ggagtgaggt tatgaaacga acgcctgtcc
     2341 tgattgatgt gaacggcgtt ccgcttcgtg agagtctcag ctacaacggg ggcggtgcag
     2401 gatttggcgg gcaaatggcg gagtggttgc caccggcgca gagtgccgat gcggccctgc
     2461 tgcccgcgtt gcgtctgggg aatgcccggg cagatgatct ggtgcgcaat aacggaatag
     2521 cggccaatgc ggtggcactg cataaggatc acattgtcgg gcatatgttt cttatcagct
     2581 accgtccgaa ctggcgctgg ctggggatgc gggagaccgc agcaaaaagc tttgtcgatg
     2641 aggtggaggc ggcctggtcg gaatacgccg aagggatgtc tggcgagatc gacgtggaag
     2701 gaaaacgcac gttcacggaa tttatccgtg aaggtgtggg cgttcatgcg tttaacggcg
     2761 aaatctttgt gcagccggtc tgggatacgg aaaccacgca gttattccgt acgcgtttta
     2821 aagccgtgag tccgaaacgg gtggacacgc caggacacgg tatggggaac cgttttctgc
     2881 gggccggggt ggaggtcgat cgatatggcc gtgccgttgc gtaccatatc tgtgaggatg
     2941 attttcctcg ctccgggagt ggacgatggg aacggatccc gcgtgaactt cccaccgggc
     3001 gtccggccat gctgcatatt ttcgagccgg tggaggacgg gcagacccgt ggggccaacc
     3061 agttttacag cgtcatggaa cggctgaaga tgctcgattc cctgcaggca acacagcttc
     3121 agtcggccat tgtgaaagcc atgtatgcag cgacgattga aagtgacctt gataccgaaa
     3181 aggcctttga atatatcgcc ggtgcgccgc aggggcagaa ggataatccg cttattaata
     3241 ttctggagaa gttctccagc tggtatgaca cgaataacgt gacgctgggt ggtgtcaaaa
     3301 ttccgcacct tttccccggg gatgatctga aactacagac tgcgcaggat tcagacaatg
     3361 gattttcggc gcttgaacag gcgctgctgc ggtatatcgc cgccggtctt ggcgtttcct
     3421 acgaacagtt gtcccgtgat tactcgaagg tcagttattc aagtgccagg gcctctgcca
     3481 atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg ctggccacgc
     3541 agatgttttc ctactggctg gaagaggcac ttcttcgggg gattatccgt ccgccacggg
     3601 cgcgttttga tttttatcag gcgcgatcag cctggtcacg ggcagagtgg attggtgccg
     3661 gaagaatggc cattgacggg ctcaaggagg ttcaggaatc ggtgatgcgc attgaggccg
     3721 gactgagcac gtatgagaaa gagccggcgc tgatgggcga ggattatcag gacattttcc
     3781 gccagcaggt cagggaatct gcagagcggc aaaaagccgg actctcacgt ccggtgtgga
     3841 tagcgcaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag gagacaacac
     3901 cccgtgagac gtaatctttc acacattatt gccgcagcat tcaatgaacc gctgcttctg
     3961 gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gcgagatggg ggcagcaagt
     4021 ctttcggtac cacagcagca ggtacagctt gatgctcccg gaatgctggc tgaaacggac
     4081 gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc gggtggtgaa cggtattgct
     4141 gtactgccgg tgaccggcac gctggtgcac cggctggggg gtatgcggcc attttccgga
     4201 atgacaggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga tagccaggtg
     4261 cggggcgtac tgctggacat tgacagtccg ggcgggcagg ccgccggcgc gtttgactgc
     4321 gctgacatga tttaccgcct ccgtcagcag aagccggtct gggcactgtg caatgacacg
     4381 gcctgttctg cagccatgct gctggcgtcg gcctgctccc gacggctggt tacccagaca
     4441 tcccgtatcg gctccattgg cgtgatgatg agccatgtca gctatgccgg tcatctggcg
     4501 caggccggtg tggatatcac gctgatttac tcaggggcgc acaaggtgga tggcaatcag
     4561 tttgaagcct taccggcaga ggttcgccag aacatgcagc agcgcattga tgcggcgcgc
     4621 cggatgtttg ccgaaaaagt ggccatgttt accggtctgt ctgttgatgc cgtcacggga
     4681 acagaggccg ccgtttttga aggtcagtcc ggcattgatg ccgggctggc ggatgaatta
     4741 gtcaatgcgt cggatgccat cagtgtgatg gccacggcgc tgaacagtaa tgtcagagga
     4801 ggcactatgc cgcaattaac tgcaacggaa gccgccgcgc aggagaacca gcgagtgatg
     4861 gggatcctga catgccagga agcgaaagga cgtgaacagc ttgccacgat gctggcagga
     4921 caacagggca tgagcgttga acaggcccgg gcgattctgg ccgcggcggc accgcagcag
     4981 ccggtggcat ccacgcagag tgaagccgat cgcattatgg cgtgtgaaga agcgaacggt
     5041 cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga aaaagcccgc
     5101 ccgatcctgg ctgcttcacc gcaggcggat gccggaccct cactccgtga tcagatcatg
     5161 gcactggatg aggcaaaagg ggctgaggcg caggctgaac agctggctgc ctgcccggga
     5221 atgactgtgg agagcgcccg ggctgtgctg gctgcgggat caggtaaggc agaaccggtc
     5281 tctgcatcca caaccgccct gtttgaacgc atcatggcga accattcacc ggctgcggta
     5341 cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat gctcatggcc
     5401 atgccatgaa gtcagtgctg accatcaaca ggaggttttt acaatatggt aacgaaaaac
     5461 atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc tcataccgcc
     5521 acaggcagca gcgggatttc ctcggcaaca ccggcactga cgcccctgat gctggatgaa
     5581 gccagcggga aactggtggt ctgggacgga cagaaagccg gtagtgcagt tggcatactg
     5641 gtactgccgc ttgaaggcac agagacggta ctgacctatt acaagtcggg gacctttgcg
     5701 acggaggcaa tccgctggcc tgaaagtgtg gatgaacaca aaaaggcaaa tgcctttgcc
     5761 ggcagtgccc tgagtcacgc ggcgctgccg taacacgtta tcaggccacc gcggtggcct
     5821 gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg ccagttactc
     5881 ggttataccg aacaaaaagt gaaatttcgt gcgctgtttc tggagctgtt tttccgccgt
     5941 acggtgaatt tccataccga agaggtgatg ctggacaaaa ttaccggaaa aacgccggtg
     6001 gcggcctatg tctccccggt tgttgaagga aaagtgctgc gtcatcgcgg tggtgaaacc
     6061 cgcgtgttac gtccgggcta cgtcaagccg aaacacgaat tcccctggag ccggtaaaag
     6121 gagccggtac caccctgtgg gtttataacg gtcagggtga cgcctatgca aacccgttgt
     6181 cagacgatga ctggcagcga ctggctaagg tgaaggatct gacgccgggc gagatgacgg
     6241 cagaatccta cgatgataac tacctggatg atgaagacgc ggactggacc gcgaccgggc
     6301 aggggcagaa atctgcaggt gataccagtt ttacgctggc ctggaaaccg ggagaggaag
     6361 gccagaaagg g
//
LOCUS       DBFIFS010000238         6334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.238, whole genome shotgun sequence.
ACCESSION   DBFIFS010000238 DBFIFS010000000
VERSION     DBFIFS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.238"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(56..2089)
                     /gene="betT"
                     /locus_tag="N7A07_004164"
     CDS             complement(56..2089)
                     /gene="betT"
                     /locus_tag="N7A07_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU2478714.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2218..2805
                     /gene="betI"
                     /locus_tag="N7A07_004165"
     CDS             2218..2805
                     /gene="betI"
                     /locus_tag="N7A07_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU2478715.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2819..4291
                     /gene="betB"
                     /locus_tag="N7A07_004166"
     CDS             2819..4291
                     /gene="betB"
                     /locus_tag="N7A07_004166"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU2478716.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4305..5993
                     /gene="betA"
                     /locus_tag="N7A07_004167"
     CDS             4305..5993
                     /gene="betA"
                     /locus_tag="N7A07_004167"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU2478717.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1536 a         1772 c         1753 g         1273 t
ORIGIN      
        1 gagtcaatga ttcccgcgcc atatgcagac atggcgcggt tttatgcaat aacaatcacg
       61 cgtccgggaa catcacgcta tggcccggcg cttcacgatg gagatgaata aagttaaggt
      121 gccgctcgta ctggtcaaga atatcggtga tcacctgctc tttgctgtag tccatcaggt
      181 cgttgccctg gctgccttct aacaggaagg tttccagccg gtagtaggtc gatttcccgc
      241 tacgtgcgcg gtaggtaaag cccggcaccg agtattgctg cggccaaatc tgatagacaa
      301 agttttgctc ttcgcccata tgcaccaaca aatccagatg ccccaactgt tgtccctctt
      361 ccggcggcag gctttttagc tccacgtacg cgccgcgcaa ccgcaactcc tgcgccactt
      421 cttccattgc cgggtaacag accgtctcca tcatctgttt agtgtaacgc gtgcccggat
      481 aattcatcag gcgcgagaga cgttttttcc agctcaggcg atcctgaagc cccagcggtc
      541 gcggtgcggt atcgcggttg gcactttcac ggcggtaatc ttctaccttc agagatttat
      601 acaaccccgc catcacgaag aagatcacaa agctgaacgg cagccccata atcaccgtgg
      661 tgttttgcag cgcggatatc ccgttggtca tcagcatgcc gagcgtcagc aggccaatcg
      721 ccaccgacca gaagacgcgc agccagccgg gggcgtcgct gttgatatct ttaagctgcg
      781 aggtgaaatt ccccagcacc agcgccccgg agtccgccga ggtcacataa aacagcaggc
      841 cagtaatggt ggcgacggag gcgctaaagg taaacgccgg atactgcgcc agcaggctgt
      901 agaagccgcg ctccggatgg accatcgctt cctcggcaaa tgccgcgccg ccgtggatga
      961 tttcatacag cgcgctattg ccgaacaccg agagccataa cagcgtgaag gtaaacggaa
     1021 taatcaacgt gcccagcacg aactggcgaa tggtacgccc acgcgagata cgcgccagga
     1081 acaagccgac aaacggcgac catgccaccc accatgccca gaagaagagc gtccagttat
     1141 tcatccactc aaccgggcgg tcgaaggcaa aactgttgag cgtcatgccc ataaagcgat
     1201 tcacatagtc gccaacattc agcaccagtg cattaagcag gaacgaagtg tcgcccataa
     1261 acaacacgaa caggatcaat cccagcgcca gcgcgacatt aagctccgat aacacgcgaa
     1321 tgcccttatc gacaccggat gtcaccgaga tagtggcgat tatcaccgac aaggcgatca
     1381 gtgccgcttt tgccgccatc gaatcgggaa tatcaaacag tacgctcaag ccatagttaa
     1441 gctgcaccac accgataccg agcgtagtgg caataccgaa gatagtgccg atcaccgctg
     1501 caatatccac tgagtgacct atcggcccgt taatccgttt accgaagatc gggtacagcg
     1561 ccgagcggat ggtgagcggc aaattataac gatagctaaa gtatccgagc gccatgccca
     1621 tcagcgcata catcgaccag ccggttaagc cgtagtgaaa cagcgtccag accatcgcct
     1681 gacgcgcggc ctcaatcgtc tgtcccgcgc cttccggcgg ctgcatatac tgcgttaccg
     1741 gttcggctac ggaaaagaac atcagatcga taccgatccc ggcagcaaac agcatcgccg
     1801 cccaactcag caggctgaat tccggtttgg attgttctgg cccgagcttc accgaaccaa
     1861 aacgcgaaca agcgatacag accacaaaga caatatagag cgttgccgcc agcagatagt
     1921 accaaccgaa ggttttagaa acccagtcca gcgtgcggcc aatccacagg gccgagaagt
     1981 cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag tccggcggag gtgtaaaaca
     2041 ccaccggatt gattttgtcc ttttccctgc tgtgtgaaag gtctgtcatc cagtatcccc
     2101 actgttattg ttaccattta aaatcaaatt cgtaacaatt aagacacatt ttatattgaa
     2161 cgtccaatca ataaccgctt taatagataa acaacgctga tgaatggagt ggcaaaaatg
     2221 cccaaattgg ggatgcagtc gatccggcgc agacaactga tcgacgccac actggaagca
     2281 ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg tgcaggcgtt
     2341 tctacgggga tcatcagcca ctatttcagg gacaaaaatg gtctgctgga agcaaccatg
     2401 cgcgatatca ccagtcagct gcgtgacgcg gttttgaatc gattacatgc acttccgcag
     2461 ggcagtgcag agcagcgatt acaggcgatt gttggcggaa acttcgatga aacgcaggtg
     2521 agcagtgcgg cgatgaaagc ctggctggcg ttctgggcca gcagtatgca tcagccgatg
     2581 ctctatcgtt tacagcaggt cagcagccgc cgcttgctgt cgaatctggt gagcgagttt
     2641 cgccgcgaat tgccgcgcca ccaggcacag gaagcgggct acggccttgc cgcgctgatt
     2701 gacggattat ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc tctcgcacac
     2761 tcgctgaccc gtcactttat tactcagcat ttacccaccg attaaccgag gagacgtgat
     2821 gtcccgaatg gcagaacagc agctttatat acatggtggt tatacctccg ccaccagcgg
     2881 tcgcaccttc gagaccatta acccggccaa cggtaacgtg ctggcgaccg tgcaggccgc
     2941 cgggcgcgag gatgtcgatc gcgccgtgaa gagtgcccaa caggggcaaa aaatctgggc
     3001 ggcgatgacc gctatggaac gctcgcgtat tctgcgtcgg gccgtcgata ttctgcgcga
     3061 gcgcaatgat gaactcgcca aactggaaac cctcgacacc ggaaaagcgt attcggaaac
     3121 ctcaaccgtc gatatcgtta ccggtgcgga cgtgctggag tactacgccg ggctgatccc
     3181 gtcgctggaa ggcagccaga tcccgttgcg tgagacatca tttgtctaca ctcgccgcga
     3241 accgctgggc gtggtggcag ggattggcgc atggaactac ccgatccaga ttgccctgtg
     3301 gaaatccgcc ccggcgctgg cggcgggtaa tgcgatgatt ttcaaaccga gcgaagtcac
     3361 tccgcttacc gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc cggacggtgt
     3421 atttaacgtg ttgccgggcg tgggcgcgga gaccgggcaa tatctgaccg agcatccggg
     3481 cattgccaaa gtgtcgttta ccggcggcgt cgccagcggc aaaaaagtga tggctaactc
     3541 ggcagcctct tccctgaaag aagtgaccat ggaactgggc ggtaaatcac cgctgatcgt
     3601 tttcgacgat gcggatctcg atctcgccgc cgatatcgcc atgatggcaa acttcttcag
     3661 ctccggtcag gtgtgtacca atggcacccg cgtcttcgtt ccggcgaaat gcaacgccgc
     3721 atttgagcag aaaattctgg cgcgcgttga gcgcattcgc gcgggcgacg ttttcgatcc
     3781 gcaaactaac ttcggcccgc tggtcagctt cccgcatcgc gataacgtgc tgcgctatat
     3841 cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac tgaaaggcga
     3901 tggcctcgat aacggcgcat gggttgcacc gaccgtgttc accgattgca gcgacgagat
     3961 gaccatcgtg cgtgaagaga tcttcgggcc ggtgatgtcc attctgacct acgagtcgga
     4021 agacgaagtc attcgccgcg ccaatgatac cgactacggc ctggcggcgg gtatcgtgac
     4081 ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta tttgctggat
     4141 caacacctgg ggcgaatccc cggcagagat gcccgttggc ggctacaaac actccggtat
     4201 tggccgcgag aacggcgtga tgacgctcca gagttacacc caggtgaagt ccatccaggt
     4261 tgagatggct aaattccagt ccatattcta accaggaggt ttatttgcaa tttgactaca
     4321 tcattattgg tgccggctca gccggcaacg ttctcgctac ccgtctgact gaagatccga
     4381 atacctccgt gctgctgctt gaagcgggcg gcccggacta tcgctttgac ttccgcaccc
     4441 agatgcccgc tgccctggca ttcccgctac agggtaaacg ctacaactgg gcttacgaga
     4501 cggaacctga accgtttatg aataaccgtc gcatggagtg cggacgcggt aaaggcctgg
     4561 gtggatcgtc gctgatcaac ggcatgtgct atatccgtgg caacgcgatg gatctcgaca
     4621 actgggcaaa agaacccggt ctggagaact ggagttatct cgattgcctg ccctactacc
     4681 gcaaggccga gacccgcgac gtgggcgaga acgactacca cggcggcgac ggcccggtga
     4741 gcgtcaccac ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt gaagcgggcg
     4801 tgcaggcggg ctacccgcgc acggacgatc tcaacggtta tcagcaggaa ggtttcggcc
     4861 cgatggatcg caccgtcacg ccgcagggcc gccgcgccag caccgcgcgc ggttatctcg
     4921 atcaggccaa atcgcgccca aacctaacca ttcgtactca cgccatgacc gatcacatta
     4981 tttttgactg taaacgcgcg gtgggcgtcg agtggctgga aggcgacagc accattccga
     5041 cccgcgcgac ggcgaacaaa gaagtgctgt tatgtgcagg cgcgattgcc tcaccgcaga
     5101 tcctgcaacg ctctggcgtc ggcaacgctg aactgttggc cgagtttgat attccgctgg
     5161 tgcatgattt acccggcgtc ggtgaaaatc ttcaggatca tctggagatg tatctgcaat
     5221 atgagtgcaa agaaccggtt tccctctacc ctgccctgca gtggtggaat cagccgaaaa
     5281 tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac tttgaagcag
     5341 gcggatttat tcgcagccga gaggaatttg cgtggccgaa tattcagtat cacttcctgc
     5401 cggtagcgat taactataac ggctcgaatg cagtgaaaga gcacggcttc cagtgccacg
     5461 tcggctcgat gcgctcgcca agccgtgggc atgtgcggat taaatcccgc gacccgcacc
     5521 agcatccggc aattctgttt aactacatgt cgcacgaaca ggactggcag gaattccgcg
     5581 acgcaattcg catcacccgc gagatcatgc atcaacccgc gctggatcag tatcgtggcc
     5641 gcgaaatcag ccccggcacg gaatgtcaga cggatgagca gctcgatgag tttgtgcgta
     5701 accacgccga aaccgccttc catccgtgcg gtacctgcaa aatgggctac gacgagatgt
     5761 ccgtggttga cggcgaaggc cgcgtgcatg ggctggaagg cctgcgcgtg gtggatgcgt
     5821 caattatgcc gcagattatc accgggaatt tgaacgccac gacgattatg attggcgaga
     5881 aaatggcgga tatgattcgc gggaaggaag cgttgccgag gagcacggcg ggatattttg
     5941 tggcaaatgg gatgccagta agagcgaaaa aaatgagtcg tgatttgaac tgactcagga
     6001 accgatcaga ttgagagtta ccgttccagg tatggggaca gaatcgttat ataaaacata
     6061 ggggtaactc tcatgcttca tagtgacaac ctcgccagca aattctaagc tgactcgttc
     6121 aatctcgctg atgaaggtac tatgaatgca ctgataaacc gcaggtgaaa tgccactcag
     6181 cttaagaatt acgccgctag tgttcctggc tatggtgact tgttcactcc cgctcacggt
     6241 tagcatatac gaaaagggtt cttgtaacga ggacaaaaat aagtcagacc aaaacaacgt
     6301 ggagctttaa acacaattcg tataatttat tagc
//
LOCUS       DBFIFS010000239         6315 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.239, whole genome shotgun sequence.
ACCESSION   DBFIFS010000239 DBFIFS010000000
VERSION     DBFIFS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.239"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..336
                     /gene="ydjO"
                     /locus_tag="N7A07_004168"
                     /pseudo
     CDS             1..336
                     /gene="ydjO"
                     /locus_tag="N7A07_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(340..1731)
                     /gene="tcyP"
                     /locus_tag="N7A07_004169"
     CDS             complement(340..1731)
                     /gene="tcyP"
                     /locus_tag="N7A07_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU2478718.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1864..2454)
                     /gene="ydjM"
                     /locus_tag="N7A07_004170"
     CDS             complement(1864..2454)
                     /gene="ydjM"
                     /locus_tag="N7A07_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2478719.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2617..3285)
                     /gene="hxpB"
                     /locus_tag="N7A07_004171"
     CDS             complement(2617..3285)
                     /gene="hxpB"
                     /locus_tag="N7A07_004171"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU2478720.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3432..3968
                     /gene="yniB"
                     /locus_tag="N7A07_004172"
     CDS             3432..3968
                     /gene="yniB"
                     /locus_tag="N7A07_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU2478721.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4009..4869)
                     /gene="yniA"
                     /locus_tag="N7A07_004173"
     CDS             complement(4009..4869)
                     /gene="yniA"
                     /locus_tag="N7A07_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU2478722.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4975..5265)
                     /gene="ghoS"
                     /locus_tag="N7A07_004174"
     CDS             complement(4975..5265)
                     /gene="ghoS"
                     /locus_tag="N7A07_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU2478723.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5366..6295)
                     /gene="pfkB"
                     /locus_tag="N7A07_004175"
     CDS             complement(5366..6295)
                     /gene="pfkB"
                     /locus_tag="N7A07_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU2478724.1"
                     /translation="MVRIYTLTLAPSLDSATITPQICPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1629 a         1578 c         1550 g         1558 t
ORIGIN      
        1 tgcgattcaa aaaaatgaga gtctcctaaa gtttccttcg ataatatcaa tgcttaatat
       61 caatcttctt tttaatccac taaatcttcc gggaatggga agtggtatac ttgaagatat
      121 tatgagtatt ccagatagtt cacttagaaa acgtctcggg tatgaggttt taagcttctc
      181 gctacaggca cattcattga gccaggaatg tattgataag ttggacattt tttcgctgac
      241 gatctgttta aatatgagtc agtatgcatc gccgcgatgg aacatttaaa aagtaaggca
      301 acggcccctt tacaaaacgg accgttgcca gcataagaat taatggtgtg ccagttcggc
      361 gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc agccactggc tggtcagcgt
      421 gccagctgtc atcgagccac taacgtttag cgccgtacgg cccatgtcga taagcggttc
      481 aacggagatt aacagcgcca ccagggttac tggcaggccc atcgcaggca gcacaatcag
      541 tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggagctaa cggtaacaat
      601 accgaccagc gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg taggcgcaac
      661 catcaccgcc agcattgccg gatacaaacc ggcgcagccg ttctgaccaa tggtcgcacc
      721 gaaagaggcg gcgaaactgg cgatggattc aggaacaccg agtcgacgcg tttgtgcttc
      781 cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg tcagcacagg
      841 ccatacctta cggaagtact tcagcggact cacgccatta atgcccagca gaatgccatg
      901 cactgcaaac ataatcagca gaccgaggta ggacgcgaca acgaaacttc ccagtttgat
      961 gatgtcttgc aggttagaac ctgcaaccac tttggtcatt agtgccagaa cgccgtaagg
     1021 ggtcaactgc atgaccaggc gaactagttt catcacccag ctttgcaggg tatcgatagc
     1081 ggttaagacg cgttcacctt tcggcgcatc atccttcagc agtttcagcg cagctacacc
     1141 gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc cggtcagatc
     1201 ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca ggtcagagac
     1261 tttaccaaca tagttacttt cgatggcgtt cagacgtgca gtttctgcac caccctgaac
     1321 cagaccttca gccgtcaaac caaacaggtt ggttaccagc acaccgacca gcgccgcaat
     1381 cagcgtggta aacaaaagcg taccaatggt cagaaaactg attttgccta actgagatgc
     1441 gttatgcaga cgggcaaccg cgctcagaat agaggcgaag actaacggca taacgatcat
     1501 ttgcagcagt tgaacatagc cgttaccaac aatgttaaac cactgtacag aatctttaag
     1561 tacctggctg tcagaaccat aaatggtatg cagggcaagg ccaaaaacta cacccatcac
     1621 cagacccacc agcacttttt tcgccagact ccactgttta tggcgggtct gagccagcgc
     1681 aaacagcagt acaacgaaca ccacgatgtt cgcaattaat ggaaagttca tccccgttct
     1741 cctgatctta ttatttctat cactgcgtag cagtcatagt gttggcgcaa gattaacaga
     1801 actgtgagct ggctcttata ttcaaatgga attgcttatt acagaaaggc gatttttgcc
     1861 tttttagtat tcaacctgat gcttaataag ccgatgaaac tggatctgca aggtattgat
     1921 catttgcgat gaccaacgaa cagcgctgtt ttcgggtaat gaatgaggca tccataccga
     1981 ccagacaaat aatgccatgc agataaaacg ttccagttgg ttaccctttt ggggaaccag
     2041 gataggcaag cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg cgggtgtcag
     2101 catatcggca agtatgtggc ttaaataacc cagcaccatt ccttgtagcg catcagccgg
     2161 aatgaaccag ctttcaggaa ccttaaggta aaaggttgcc agcagcgcaa ataccgccag
     2221 cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg atcggttttg atatccactt
     2281 taatcgctgc ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac acgtcaggat
     2341 tgcggaaggg acaatatgcc accagtcacc ctgtgccagc acgggcgtca gctcggcatt
     2401 tttggcaaat accgcacaag caatagaaaa gagaaggtga ccttccgccg tcatgataaa
     2461 actcactaaa ctgtcgatac gtacagtata ggatttttat acagtgcacg gaagtggtga
     2521 cggtgtaact ctttctctta aattgacgat taatccgtca gttgaatgca aattcagata
     2581 aagaaccagg ggcagcgtga tcactgcccc tggtcattaa ccgagaaggt cttttgcggt
     2641 gagttctgtc agcgatgaga gtttgacgtt tgctaataca aaacgtggat cattttgcgc
     2701 ttctggcgca ggaacgacga tggaacgcat gcgggctgct ttagaggcga tcatgccatt
     2761 taccgaatcc tccagcgcta cgcaggtcag tgggtcaacg cccagttttg ctgcgcagtc
     2821 gagatatact tgcggatgcg gcttgctgta aggcagtttt tcggccgagg cgagggcatc
     2881 gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc agcatatgta gtggtgacgc
     2941 ggaggccagt cccaccaata aaccttgttc tttgcataac gcaacggctt cgcgcacgcc
     3001 tggtaataat ggacgtgtct cttcaaccag tgaaatggca cgggcaataa cccgttctac
     3061 tacttcctga cggcttggcc cattccacgg ttgccgggcg taccaaagat cgaccaccat
     3121 atcgatgcgt aaacctaagg tgtccggcag ctcgttacga cgggagatat ccacccccag
     3181 gcttgccatc acatccagtt cggctcgatc ccataaaggt tctgagtcga taagtaatcc
     3241 atccatatca aaaattgcag caagaatctg acgcggggtt gacattacaa cctctccttt
     3301 agagcattat gtgtaaggta ttaagccgtg cttatgatta agcgcctatc atatcgcctt
     3361 taaagaatgg gcgaaaacgg atatctacag gtaaactaag tgaaacgacg cgtcttaatc
     3421 aaggagaact catgacgtat caacaagctg gacgcattgc tgttttgaaa aggattatgg
     3481 gttgggtgat ttttatacct gcattgatct ctacattgat ttctttgctg aaatttatga
     3541 atacccggca ggaaaaccag gaaggcatta atgcggtcat gctcgacttt actcatgtca
     3601 tgatcgatat gatgcaggcg aatacgcctt ttttgaatct gttctggtat aactccccga
     3661 cgcccaattt taatggtggc gtgaacgtta tgttctgggt gattttcatc ctgatttttg
     3721 ttggactggc gttgcaggat tccggtaccc ggatgagtcg ccaggcgcgc ttcttgcggg
     3781 aaggtgttga agatcaactc attctggaaa aagccaaagg ggaagagggg ctaacgcgtg
     3841 aacaaattga gtcacgtatt gttgttccgc accatactat tttcctgcag tttttctcac
     3901 tgtatatcct gccggttatt tgtattgctg caggttatgt gttcttttct ctgcttgggt
     3961 ttatttaatc aaaaggccgc acggagcggc cattatcttc aacccatatc acgcagctaa
     4021 taatctatcc agtgactgct gagcaataac caaatgctga ccgccaaata atcttgcacg
     4081 atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg caggtagcgg
     4141 ggatactgac tgatagccgt catagatttg tggcggctgt tcagtatgca gcggtaacat
     4201 cgccaggtcg cactctcggt caccccagta gcaggccggg tcgaaaatgt acgggccatc
     4261 cggacccagc gcacagttgc cggaccataa atcgccgtgc aacagagaag gctgcggttg
     4321 atgtgaggcg agacgctgct gaatatgctc gacgatggca tcgatattgc cgaaagcgat
     4381 ccctttttct gctgccagtt ccaactgcca gccaatccgt tgttcagcaa aaaacgttga
     4441 ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag agcgagttat caaaatcgag
     4501 tccaaattgt ggttggtcgc tccattgatg taaacgcgca atttgctgac caagaataaa
     4561 tgcgctatgc gcatccagcg gacgaggtgg gagataatcc atcaccagaa aactgtagtc
     4621 acggtcagcg ccaactgccc aaaccttagg cacggtgacg gttttactac gcgacagtaa
     4681 ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt tccctttcat cacatttgac
     4741 gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca tggacttctc cgccaggcag
     4801 ttcattacgc agttcgattt cgccttcacc taactgctcg ctcaaaagac gactgattgc
     4861 ctgccacatg atgtctctcc catgttgtct gccagatcat aaggttagcg caagaatgcg
     4921 gtgtaaaaac acgagctaac gcacaccaca gactgattat tgcccaaaaa ccatttattt
     4981 tctgttgcta ttccattcct cccaggtcgt gatggtgatt tcaggatctt tgccggttgc
     5041 actttgggta agccccgaaa ccagttctct aatttcttct tcactttggg tactgataag
     5101 tccaaaagtg ttagtcccca gctcatggat atttccctca tcatccgtca tggtgagcaa
     5161 aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg tatcctcatg
     5221 caacatgacg gttatgacat aacggacaag atcgccactg gccatagttc acccctttgt
     5281 tatcacatga cttttgagca tagtcggaga aacgcgttgc cgacaggttg gtgatgattc
     5341 ccccaatgct gggggaatgt ttttgttagc gggaaaggta agcgtaaatt ttttgcgtat
     5401 cgtcatggga gcacagacgt gttccctgat tgagtgtggc tgcactcccc gcagctacgc
     5461 caaaacgaac catctcttca agagaggcat tttctgccag tttcagtgtc atcgcgccga
     5521 ccatgctatc accagcgcca acggtactct ggcttttcac cggtggtggc accacctgaa
     5581 tgcagttttc actatcaaca cccagcgctc cttgtggacc cagggaaacg acaacccgtt
     5641 tggccttgcc gctattaacg atttcctgcg cggctttgcg gacatcgtct ggctgggtga
     5701 gttcgcgatt caccagcgca ctgagttctt tttggttagg cttaaccaac tcgatgttac
     5761 caatcgccag tgctgcactt aacgcttcgc cagaactgtc gatgatgcag cggatccctt
     5821 ggttttgcgc agcggaaatc agttgggtta atttttccag cttcacacct ggcggcaggc
     5881 ttccgcttat gaccaggatg gccccggatt caatttccag aacttgctct tcaagctggc
     5941 gaaactcatc ttcatttaat gccgcgcctg gcataacaaa acgatactgc tcaccgcttg
     6001 cttccacatg tacgtgtaaa ttctgccggg tccagtcttt ggcttctaca gtagcgacgg
     6061 ggacattttc atccgccaac agtgaaacca ggtgttcgcc ggtcgcgcca cccgccggga
     6121 agatcgctgt ggcactgcct ccaagatggg caatggcgcg ggcgacgttg atgccgccgc
     6181 ccccgggttc gaacaccggt gcggtacagc gcagttttcc ttcgggacaa atttgcgggg
     6241 taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt atagatacgt accatcattt
     6301 cctcctatag gctga
//
LOCUS       DBFIFS010000240         6277 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.240, whole genome shotgun sequence.
ACCESSION   DBFIFS010000240 DBFIFS010000000
VERSION     DBFIFS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.240"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..92)
                     /locus_tag="N7A07_004176"
     CDS             complement(<1..92)
                     /locus_tag="N7A07_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478725.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMM"
     gene            170..1264
                     /gene="ddlA"
                     /locus_tag="N7A07_004177"
     CDS             170..1264
                     /gene="ddlA"
                     /locus_tag="N7A07_004177"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU2478726.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1288..1500)
                     /gene="yaiZ"
                     /locus_tag="N7A07_004178"
     CDS             complement(1288..1500)
                     /gene="yaiZ"
                     /locus_tag="N7A07_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU2478727.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1760..2068
                     /gene="yaiY"
                     /locus_tag="N7A07_004179"
     CDS             1760..2068
                     /gene="yaiY"
                     /locus_tag="N7A07_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU2478728.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2127..3221)
                     /gene="yaiW"
                     /locus_tag="N7A07_004180"
     CDS             complement(2127..3221)
                     /gene="yaiW"
                     /locus_tag="N7A07_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU2478729.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHISAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3234..4454)
                     /gene="sbmA"
                     /locus_tag="N7A07_004181"
     CDS             complement(3234..4454)
                     /gene="sbmA"
                     /locus_tag="N7A07_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU2478730.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4806..5963
                     /gene="ampH"
                     /locus_tag="N7A07_004182"
     CDS             4806..5963
                     /gene="ampH"
                     /locus_tag="N7A07_004182"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU2478731.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5964..>6277)
                     /locus_tag="N7A07_004183"
     CDS             complement(5964..>6277)
                     /locus_tag="N7A07_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478732.1"
                     /translation="LIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLS
                     MIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSE
                     Y"
BASE COUNT         1569 a         1629 c         1541 g         1538 t
ORIGIN      
        1 ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt caccagtacg
       61 ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga atcccccgtt
      121 ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact
      181 gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc
      241 aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga
      301 taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc
      361 tgcccatatt gcgttgcgcc cttcggcgac cagcctcgcc caggtgccag gtaaacatga
      421 gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg tcattttccc
      481 gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa
      541 tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggata aagatgtcac
      601 caaacgtctg ctacgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc
      661 taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac cactgtttgt
      721 aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg aagaacaata
      781 cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg agcaagggat
      841 caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag ccagcacctg
      901 tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca ttgacgaaga
      961 tggcgcgaaa gtggtagttc cggcagccat tgcgccagaa atcaacgata agatccgggc
     1021 gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt
     1081 tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa
     1141 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac
     1201 acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     1261 gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg
     1321 acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata
     1381 ggtctgccaa ccttttgctt caaatcccag taaccccacc acgccattaa gaatgaatat
     1441 aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat
     1501 ggctgctccg ttaacgaaaa acaacatcct tgcatattct gcaacaaccg tctgtggtgt
     1561 agacaacatt ttcgcaattg ttaataaatt taacttaaag ttgctatttt gtgactcgca
     1621 acgaagattc acgcttcggg aatattccca acatcaattt accatgattc tgatattgac
     1681 aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag
     1741 atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa
     1801 atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg
     1861 cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg
     1921 tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac
     1981 cattttcggg ctgatcccgt ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg
     2041 gctgcactgg cgacatcgcc gccagtaaga tttacgttgc cagatacggc ctaaaacgcc
     2101 ttatctggca tgacattcct gtttgttcac tgtttcatac aacgcgcccg ccgttcgtct
     2161 acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga
     2221 atgccaggca acatttcgcg ggggagtgat ttacccgttt tcgcttcggc aagttgataa
     2281 actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc tagctgacga
     2341 cgaatttcgc tgtcgctcat ccccagtttt gccgccagtt tgcgcgttgc cagttcggtt
     2401 ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg
     2461 ctggccttac tgaccgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta
     2521 aaatcagcaa aacggtatat cggtgcgcta taactggcgg gataattcag taaatggtaa
     2581 gtaccaaacc acaacccgcc gcggcgacta aagacttcct gacggactgt accgtccatt
     2641 ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca
     2701 ccggtgcgca ccggattaag cgaaccaaac aacgtctgcc ccattggcac catgctgatc
     2761 agatcgtcaa aaatcccgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta
     2821 taacttttgc cgtttggcga tttgattttt agcgcagtat gaaccagaaa agcagaaatg
     2881 tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg
     2941 gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga gcagacgttt
     3001 tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca
     3061 tcgcggtctt tcacgctcgc aggcatcttc tgccgcacca cgctagccac atctatcgct
     3121 ttttcgccct ttttcaacgg ctgtggcgcc tgactgctac accccgctaa aaccagaacc
     3181 gccaaaaatg aaagcgaaga cagatgattg actcgcgaca ttacttctcc tttttagctc
     3241 aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga
     3301 cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa
     3361 gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta
     3421 ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag
     3481 agaatgcgag cgatgttgaa atacatatag tgaaaataga ggcggaaata gtttttccgt
     3541 acggcgctaa acagctcgcg taccgttggc ggcgtcgcgc gcgtggcatc gtcttcacca
     3601 taaaccagct ctttacggta ggcagcctct acacgctggt ttttaaactc cagccccggc
     3661 agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca
     3721 atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc
     3781 gttaccaaca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacaccc
     3841 atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcgcct
     3901 tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag
     3961 cggaacacgt agtgactgac aaagaagtta ttcagcacac tgatcactac cgcgatcagc
     4021 gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc
     4081 gaacttagcg cggtttgaat cagatcatag aacggtgcat accaggcgtt gacggcaacc
     4141 ccaacttcca ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag tattgaccag
     4201 tattgccaac ggtgcgggct gtagataaac cagaacaatg caaaaagtcc tacgcaaaca
     4261 atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg
     4321 atctgcccgg aagcgccggt gatacgcgcc acccagtccc ccccaccggc ttgccagaag
     4381 ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa
     4441 aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt
     4501 aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga ctcatagcaa
     4561 ggcgcttagt tcgccagcct ggccataaga attgtttcga cggatttcac aaaccaaaga
     4621 ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga
     4681 aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt
     4741 tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat ttatggacac
     4801 caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg
     4861 tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg
     4921 ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta
     4981 accagcgcgt ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc
     5041 tggattcggt cgtgcgtatt gcttccctca ccaagctaat gaccagtgag atgctggtga
     5101 aattgctcga ccaggggacc gtgaagttaa acgatccgct aagtaaatat gctccgccag
     5161 gcgcacgcgt gccaacctac aatgggacgc ccattacgct ggtcaatctg gcaacccata
     5221 ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc
     5281 caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca
     5341 gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt
     5401 ccggcaagcc ttatacccag ttgtttgaag agcagattac ccgtccattg gggatgaaag
     5461 acaccaccta cactccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg
     5521 ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg
     5581 gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc
     5641 aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca
     5701 tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag
     5761 agggtcgtcc ggggattatt cagaagacag gtggtggtgg cggtttcatt acctatatgg
     5821 cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc
     5881 gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac
     5941 cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt
     6001 ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg
     6061 acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc
     6121 aattcttcat tccagtcaat cattgataat aatgttgcgc ggatttgttc gtaggaatta
     6181 tgcccaatga gctgcacgtc gcgtaattcc agaattctat tttgccaggc taaccagtaa
     6241 aaagcgtctc gccagagttg attttgttca ataagcg
//
LOCUS       DBFIFS010000241         6246 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.241, whole genome shotgun sequence.
ACCESSION   DBFIFS010000241 DBFIFS010000000
VERSION     DBFIFS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.241"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(107..250)
                     /gene="ypaB"
                     /locus_tag="N7A07_004184"
     CDS             complement(107..250)
                     /gene="ypaB"
                     /locus_tag="N7A07_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU2478733.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            378..2663
                     /gene="nrdA"
                     /locus_tag="N7A07_004185"
     CDS             378..2663
                     /gene="nrdA"
                     /locus_tag="N7A07_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU2478734.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKHFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2890..4020
                     /gene="nrdB"
                     /locus_tag="N7A07_004186"
     CDS             2890..4020
                     /gene="nrdB"
                     /locus_tag="N7A07_004186"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU2478735.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4020..4274
                     /gene="yfaE"
                     /locus_tag="N7A07_004187"
     CDS             4020..4274
                     /gene="yfaE"
                     /locus_tag="N7A07_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU2478736.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4328..4978)
                     /gene="inaA"
                     /locus_tag="N7A07_004188"
     CDS             complement(4328..4978)
                     /gene="inaA"
                     /locus_tag="N7A07_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU2478737.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5059..>6246)
                     /locus_tag="N7A07_004189"
     CDS             complement(5059..>6246)
                     /locus_tag="N7A07_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2478738.1"
                     /translation="KEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPDK
                     AGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLFI
                     RVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWEA
                     FNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFFM
                     TFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYPC
                     LLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSLF
                     FNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVAK
                     EIH"
BASE COUNT         1617 a         1688 c         1520 g         1421 t
ORIGIN      
        1 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
       61 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      121 aacagttatt tataacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      181 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      241 agagggccat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      301 tctaagcagc ttcccgtact acaggtagtc tgcacgaaac tattgcggaa agaattccaa
      361 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      421 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      481 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
      541 cctctgacat ccacgaaacc attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
      601 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
      661 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
      721 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
      781 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcaactg gaaggcaaat
      841 atctggtaca gaaccgtgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
      901 tggttgctgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tatgtgaagc
      961 atttttacga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1021 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggc gacagcctgg
     1081 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1141 gcatcaacgc cgggcgtatt cgtgcgctgg gtagcccgat tcgcggtggc gaagcgttcc
     1201 ataccggctg cattccgttc tacaaacatt tccagactgc ggtgaaatcc tgctctcagg
     1261 gcggcgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1321 gcctgctggt gttgaaaaac aaccgtggtg tggaaggcaa ccgcgtgcgt catatggact
     1381 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1441 tgttcagccc gtccgacgta ccggggctgt acgacgcatt cttcgccgat caggaagagt
     1501 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     1561 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     1621 agaacgttga ccactgcaat acccatagcc cgtttgatcc ggccatcgcg ccagtgcgtc
     1681 agtctaacct gtgcctggag atagccctgc cgaccaaacc gctgaacgac gtcaacgacg
     1741 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     1801 tggatgaact ggaagagctg gcaattctgg cggttcgtgc acttgacgcg ctgctggatt
     1861 atcaggatta cccgatcccg gccgccaaac gtggagcgat gggtcgtcgt acgctgggta
     1921 ttggtgtgat caacttcgct tactacctgg cgaagcatgg taaacgctac tccgacggca
     1981 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2041 ctaatgagct ggcgaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2101 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgaaccgc
     2161 tgcattacga ctgggaagct ctgcgtgagt caatcaaaac acacggcctg cgtaactcca
     2221 cgctttctgc tctgatgccg tccgagactt cttcgcagat ctctaacgcc actaacggta
     2281 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ctgcgccagg
     2341 tggtgccgga ctacgagcat ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2401 acgatggcta cctgcaactg gtgggtatca tgcagaaatt tatcgatcag tcgatctctg
     2461 ctaacaccaa ctacgatccg tcacgcttcc cgtcaggaaa agtgccgatg cagcagttgc
     2521 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     2581 gtgacggcgc tgaagacgcg caggacgatc tggtgccgtc aatccaggac gatggctgcg
     2641 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     2701 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gcacaggatg
     2761 cggcgtgaac gcctaatccg gcctacggct cggatttgta ggcctgataa gacgcgccag
     2821 cgtcgcatca ggcacaggat gcggcgtaaa atgccttatc cggcattaaa ctcccaacag
     2881 gacacactca tggcatatac caccttttca cagacgaaaa atgatcagct caaagaaccg
     2941 atgttctttg gtcagccggt caacgtggcc cgttacgatc agcaaaaata tgacatcttc
     3001 gaaaagctga tcgaaaagca gctctctttc ttctggcgtc cggaagaagt tgacgtttct
     3061 cgtgaccgta tcgattacca ggcgctgcct gaacacgaaa aacacatctt tatcagcaac
     3121 ctgaaatatc agacgctgct ggactccatt cagggtcgta gcccgaacgt ggcactgctg
     3181 ccgctgatct cgatcccgga actggaaacc tgggtagaaa cctgggcgtt ctcagaaacg
     3241 attcattccc gttcctatac tcatatcatt cgtaatatcg ttaacgatcc gtctgttgtg
     3301 tttgacgata tcgtcaccaa cgagcagatc cagaaacgtg cggaagggat ctccagctac
     3361 tacgatgagc tgatcgaaat gaccagctac tggcacctgc tgggcgaagg cacccacact
     3421 gttaacggta aaaccgtgac cgttagcctg cgcgagctga agaaaaaact gtatctctgc
     3481 ctgatgagcg ttaacgcgct ggaagcgatc cgcttctacg tcagctttgc ttgttccttt
     3541 gcctttgcag aacgcgaact gatggaaggt aacgccaaaa ttattcgcct gattgcccgc
     3601 gacgaagccc tgcacctgac cggtactcag catatgctga atctgctgcg cagcggtgcg
     3661 gatgatccgg agatggcgga aattgccgaa gaatgtaagc aggagtgcta tgacctgttt
     3721 gtccaggcgg cacagcaaga gaaagactgg gcggattatc tgttccgtga cggttcgatg
     3781 attggtctga ataaagacat tctctgccag tacgttgaat acatcaccaa tatccgcatg
     3841 caggcagtcg gtttggatct gccgttccag acgcgctcca acccgatccc gtggatcaac
     3901 acctggctgg tgtctgataa cgtgcaggtt gctccgcagg aagtggaagt cagttcttat
     3961 ctggtcgggc agattgactc ggaagtggac accgacgatt tgagtaactt ccagctctga
     4021 tggctcgcgt taccctgcgc atcactggca cacaactgct gtgccaggat gaacaccctt
     4081 cccttctggc ggcgctggaa tcccacaatg tggcggttga gtaccagtgc cgcgaaggtt
     4141 actgcggctc ctgtcgcaca cgcctggttg caggccaggt ggactggatt gccgaaccgt
     4201 tagcctttat tcagccgggg gaaattttgc cctgttgttg ccgagcaaaa ggcgatattg
     4261 aaatcgagtt gtgatcgggt actagcgcca gatacgacgc ttacgcgtat tatctggcct
     4321 acaacgatta catggcgtag taatacgctt ttacctgctc ccagtccgct ttggggatcg
     4381 gctccagata tttttccagc tggcggaagt catgattaat cgctttatcg cgacgcaagc
     4441 ggcgacgact tttctccaga tcaagaaaac cagcttctgc cttaccttct gttttcacat
     4501 agatatggcg aacataacaa cagccatgct gacgattaac gctatgcatt ttcttaaacg
     4561 ccagcgccac tgctttcaac atggcttgcc gtacttcgtc agaatagggc gttactgcat
     4621 gctgggcata ccagtcagca atgctgatga accccgccat atcttcagtc accagcaacg
     4681 cgcgccattc cccctcaatt ttcaccgctt caccaaaaac gatcttcggc acgatgacac
     4741 cggcccgttc cagttcttta attaccgcaa cctcacggac aatcgttggt cggccgaaag
     4801 gataacgtac ggaatgaaac agatgatgcg tcatgcgctt tacatatagc tttttgccgt
     4861 tgcgctcgac gcattgcacc ccgctcatac cattacggcg atagttaggc tcttcaaccc
     4921 agtcgccctc tgttgcccac cagtgattaa attcgtcgtg ctttgctgaa actgccatac
     4981 cctatcgcct gtcatttttt attaacgaca atgactatag gtggttacct gcgaaaaatc
     5041 ttaatgaaac gtgtcgtatt aatgaatttc ttttgccact actagcgcga caccgctttt
     5101 tcccttcatt tgcagcgcat agactccacc agccactacc actaatagtg cgcccagcag
     5161 ataaatcatt cggtagccag tatgtgcaac catcatcccc atcacacagg ctcccagcgc
     5221 caggcctgca tccatcgcat tgaagaagag ggagtttgcg acaccgattt tatgcggttc
     5281 cactgaactg ataatctgcg tctgaaaaac tggcgtcacg gaaccatacc cgataccaat
     5341 caacgcgcca gaaacgacca tcattacgct gccctgggtg tagccgagaa ccaccagccc
     5401 aaccgtaaag aacagcaggc aggggtaaac cacatatttc gggccttttt tgtcgcaaac
     5461 attgccggtg aaggtacggc agatcatcag gaagatggcg tagcagagca ggaaattact
     5521 ggccgccgac atcagattaa gttcgcgggc gtaaagcgcc agaaaggcag aaaccccggc
     5581 atacgaaaag gtcataaaga atgtaaccat ggcaaacggc aatgccgcgc gatcgaacat
     5641 ggcggcgaag cccatctttg gctttgaacc gtcggcatgg ctgataaccg gcggcacggt
     5701 cataatcagc gacaggataa tccccaccgc cgcaacgcca gtacataacc agaaagccat
     5761 attaaaggcc tcccagcgcg ccatattcag gccaatccac ggcccgacca ccattgccag
     5821 ccccattgcc agagagaaaa aactgatgcc ctccccacgt cgggaggccg ggatcagccg
     5881 cgcggaaatc gttcccttta ccgttgtgat aaccccgaag gtaataccat gaagcaccct
     5941 aataaaaagc agtgattcta ttgagtggca aaaaggatac agcgcagtga ccaccaaaaa
     6001 ggccagagaa gagagcacca gaatagtttt attcgaatat ttacccaccc attgcccggc
     6061 aaagggtcga ataacaatcg ccgcaattaa aaataaagtc accaacaaac ctgctttatc
     6121 aggagaggca tgcaagcggt cagagatata aattggcaac aatgttagta aaacataaaa
     6181 gacgaagaaa ataataaagc tgatgatggt tattgcccaa aaatccttcg tccataactt
     6241 ctcttt
//
LOCUS       DBFIFS010000242         6240 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.242, whole genome shotgun sequence.
ACCESSION   DBFIFS010000242 DBFIFS010000000
VERSION     DBFIFS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.242"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1647
                     /gene="hcp"
                     /locus_tag="N7A07_004190"
                     /gene_synonym="priS"
     CDS             <1..1647
                     /gene="hcp"
                     /locus_tag="N7A07_004190"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU2478739.1"
                     /translation="CVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQGLS
                     AWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQCLA
                     VDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGA
                     AAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDA
                     GETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAH
                     GYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIV
                     GWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVSRE
                     KLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLPRL
                     VDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVKNI
                     VTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            1659..2627
                     /gene="hcr"
                     /locus_tag="N7A07_004191"
     CDS             1659..2627
                     /gene="hcr"
                     /locus_tag="N7A07_004191"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU2478740.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            2760..4478
                     /gene="poxB"
                     /locus_tag="N7A07_004192"
     CDS             2760..4478
                     /gene="poxB"
                     /locus_tag="N7A07_004192"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU2478741.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            4515..5516
                     /gene="ltaE"
                     /locus_tag="N7A07_004193"
     CDS             4515..5516
                     /gene="ltaE"
                     /locus_tag="N7A07_004193"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU2478742.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            5527..>6240
                     /locus_tag="N7A07_004194"
     CDS             5527..>6240
                     /locus_tag="N7A07_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU2478743.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLP"
BASE COUNT         1423 a         1685 c         1742 g         1390 t
ORIGIN      
        1 tgtgtgcaat gtgaacaaac tatccgtact ccggcaggaa acggctgctc atacgcgcag
       61 gggatgtgtg gtaaaacggc ggaaacttct gaccttcagg atttactcat cgcggcgctg
      121 caagggcttt ctgcctgggc ggtaaaagcg cgtgaatacg gcatcatcaa ccacgatgtc
      181 gacagcttcg cgccacgtgc ctttttctca accctgacca acgttaactt cgattctccg
      241 cgtattgtcg gctacgctcg tgaagcgatt gccttgcgcg aggcgctgaa agcacaatgc
      301 ctggctgtag atgccaacgc ccgcgtcgat aacccgatgg ctgacctgca actggtgagc
      361 gacgatctcg gcgaactgca acgtcaggca gcagaattta cccctaacaa agataaagcg
      421 gcgattggcg aaaacattct cggcctgcgt ctgctgtgcc tgtatggcct gaaaggtgcg
      481 gcggcctata tggaacacgc acacgttctc ggtcaatacg acaacgatat ttatgcccag
      541 tatcataaaa tcatggcgtg gctggggacc tggcctgccg atatgaacgc gcttcttgag
      601 tgttcaatgg aaatcggcca gatgaacttc aaagtgatga gcattctgga tgcaggcgaa
      661 accggtaaat acggtcaccc gacgccaacc caggtcaacg tcaaagcgac ggcgggtaaa
      721 tgcatcctga tttccggtca cgatctcaaa gatctctaca acctgctgga acagaccgaa
      781 ggcacgggcg ttaatgtcta cacccacggc gaaatgctgc ctgcgcatgg ctacccggag
      841 ctgcgtaaat tcaagcatct ggtcggtaac tacggcagcg gctggcagaa tcagcaagtg
      901 gagttcgctc gtttccctgg ccccatcgtg atgacctcga actgcatcat cgacccaacc
      961 gtaggcgctt atgacgatcg tatctggacc cgcagcatcg ttggctggcc tggcgtgcgt
     1021 catctggatg gcgaagattt ctctgcggtc attgcccagg cgcaacaaat ggcaggcttc
     1081 ccatacagcg aaattccgca cctgatcacc gtgggctttg gccgccagac gctgcttggc
     1141 gcagcggata cgctaattga tctggtgagc cgtgaaaaac tgcgtcatat cttcctgctt
     1201 ggtggctgtg acggcgcacg cggcgaacgt cactacttca ccgatttcgc caccagcgtg
     1261 ccagatgact gcctgatcct gactctcgcc tgtggtaaat atcgctttaa caaacttgag
     1321 tttggcgata tcgaaggtct gcctcgtctg gtggatgccg gtcagtgtaa cgatgcttac
     1381 tcagcaatta ttctggcggt tacgctggca gaaaaactgg gctgcggtgt caacgatctg
     1441 ccgctgtcgc tggtgctctc ctggtttgaa cagaaagcga ttgtcattct gctgacgctg
     1501 ctttctctgg gcgtgaaaaa tatcgtcacc ggcccgactg cacctggttt cctgacacca
     1561 gatctgctgg cggtgctgaa cgagaaattc ggcctgcgtt ctatcaccac tgttgaagaa
     1621 gacatgaagc aactgttgag cgcgtaagga gtttgtcgat gacgatgcca acgaatcaat
     1681 gcccgtggcg gatgcaggtt catcacatta agcaagaaac gccggatgtg tggacgattt
     1741 ccctgatttg ccacgattac tacccatacc gcgccgggca atatgcactg gtcagcgtgc
     1801 gtaactcagc ggaaacgctg cgtgcttaca ccatttcctc cacgccaggc gtgagtgaat
     1861 atatcaccct gaccgtgcgg cggattgatg acggtgtcgg ctcccagtgg ctgacccgcg
     1921 atgtaaaacg cggtgattat ctctggcttt cagacgcgat gggggaattt acctgcgacg
     1981 ataaagcaga agataaattc ctgttgctgg ctgcaggctg cggcgtcacg ccgattatgt
     2041 cgatgcgtcg ctggctggcg aagaaccgtc cacaggccga tgtgcgggtg atctacaacg
     2101 tgcgtacgcc gcaggatgtt attttcgccg atgagtggcg taactatccg gtaacgctgg
     2161 tggcggaaaa taacgttacc gaaggcttta tcgctggtcg tctcactcgc gaactcctga
     2221 ctcgcgttcc tgacttagct tcacgtaccg tgatgacctg cggcccggct ccgtatatgg
     2281 attgggtaga gcaggaagtg aaagcactcg gcgtgacgcg tttctttaaa gagaaattct
     2341 tcaccccagt agcggaagct gcgaccagcg gtctgaaatt caccaaactg caaccggcgc
     2401 aagaatttta cgctcctgtt ggcaccacgc tgctggaggc gctggaaagc aataacgttc
     2461 cggttgtcgc cgcctgccgc gcgggtgttt gcggctgctg taagacgaaa gtggtttccg
     2521 gtgaatatac ggtgagcagt acaatgacgc tgaccgacgc cgaaatcgct gaaggttacg
     2581 tactggcctg ctcctgccat ccgcaggggg atttggttct cgcataatcg ccttatgccc
     2641 gatgatattc ctttcatcgg gctatttaac cgttagtgcc tcctttctct cccatccctt
     2701 ccccctccgt cagatgaact aaacttgtta ccgttatcac attcaggaga tggagaacca
     2761 tgaaacaaac ggttgcagct tatatcgcca aaacactcga atcggcaggc gtgaaacgca
     2821 tctggggagt cacgggcgac tctctgaacg gtcttagcga cagtcttaat cgcatgggca
     2881 ccatcgagtg gatgtccacc cgccatgaag aagtggcggc gtttgccgct ggcgctgaag
     2941 cacaactttc aggagaactg gcggtctgtg ctggatcgtg cggccccggc aacctgcact
     3001 taatcaacgg cctgttcgat tgccaccgca atcacgttcc ggtactggcg attgccgctc
     3061 atattccttc cagcgaaatt ggcagcggct atttccagga aacccaccca caagagctat
     3121 tccgcgaatg tagtcactat tgcgagctgg tttccagccc ggagcagatc ccacaagtgc
     3181 tggcgattgc catgcgcaaa gcggtgctta accgtggcgt ttccgttgtc gtgttaccgg
     3241 gcgacgtggc gttaaaacct gcgccagaag gggcaactac ccactggtat catgcgccac
     3301 agccagtcgt gacgccggaa gaagaagagt tacgcaaact ggcgcaactg ctgcgttatt
     3361 ccagcaatat cgctctgatg tgtggcagcg gctgcgcggg ggcgcataaa gagttagttg
     3421 agtttgccgg gaaaattaaa gcacctattg ttcatgccct acgcggtaaa gagcatgtcg
     3481 aatacgataa tccgtatgat gttggaatga ccgggttaat cggattctcg tcaggtttcc
     3541 ataccatgat gaacgccgac acgttagtgc tactcggtac tcagttcccc taccgtgcgt
     3601 tctacccgac cgatgccaaa attattcaga ttgatatcaa cccagccagc atcggcgcac
     3661 acagcaaggt ggatatggcg ctggtcggcg atatcaagtc gaccctgcgt gcattgctgc
     3721 cattggtgga agaaaaagcc gatcgcaagt ttctggataa agcgctggaa gattaccgcg
     3781 atgcccgcaa agggctggac gatttagcta aaccgagcga gaaagccatt cacccgcaat
     3841 atctggcgca gcaaattagt cattttgccg ccgatgacgc catctttacc tgtgacgtcg
     3901 gcacgccaac ggtgtgggcg gcgcgttatc tgaaaatgaa cggcaagcgt cgcctgttag
     3961 gttcgtttaa ccacggttcg atggctaacg ccatgccgca ggcgctgggc gcgcaggcaa
     4021 ccgagccgga acgtcaggtg gtcgccatgt gcggcgatgg cggttttagc atgttgatgg
     4081 gcgatttcct ctcggtagtg cagatgaaac tgccggtgaa aattatcgtc tttaataaca
     4141 gcgtgctggg ctttgtggcg atggagatga aagccggagg ctacctgaca gacggtactg
     4201 agctgcacga caccaacttt gcccgcattg ccgaagcgtg cggaattacg ggtattcgtg
     4261 tagaaaaagc ctctgaaatc gatgaagccc tgcaacgcgc cttctccatc gacggtccgg
     4321 tattggtgga tgtggtggtc gccaaagaag agttagccat tccaccgcag atcaaactcg
     4381 aacaggccaa aggtttcagc ctgtatatgc tgcgcgcaat catcagcgga cgcggtgatg
     4441 aagtgatcga actggcgaaa acgaactggc taaggtaaaa agggtggcat ttcccgtcat
     4501 aataaggaca tgccatgatt gatttacgca gtgataccgt aacccgaccg agccgcgcca
     4561 tgctcgaagc gatgatggcc gccccggttg gggacgacgt ttacggagac gaccctaccg
     4621 ttaatgctct gcaggactat gcagcagagc tttccggtaa agaagccgcc atttttctgc
     4681 ctaccggcac tcaggccaac ctggtcgctc tgctcagtca ctgcgaacgc ggcgaagagt
     4741 atattgtcgg tcaggccgcg cataactatc tgtttgaagc cggtggcgcg gcggtgctgg
     4801 gcagtattca accgcaaccc atcgacgcgg ctgccgacgg cacgctaccg ctggataaag
     4861 tggcgatgaa aatcaaaccc gacgatatcc atttcgcccg caccaaatta ctcagtctgg
     4921 aaaacaccca caacggcaaa gtgttgccgc gtgaatacct taaagatgca tgggaattta
     4981 cccgcgagcg caatctggcg ctgcatgttg acggtgcgcg catctttaat gccgtggtgg
     5041 cttacggcag cgaactgaaa gagctcacgc aatattgtga ttcgttcacc atttgcctgt
     5101 cgaaaggtct tgggacgcca gtcggttcat tactcgtcgg taatcgtgat tacattaaac
     5161 gtgccattcg ctggcggaaa atgacaggtg gcgggatgcg ccagtccggc attctggctg
     5221 ccgccgggat gtatgcgctg aaaaataacg tcgcacggtt gcaggaagat cacgacaacg
     5281 ccgcctggat ggcggagcaa ctgcgtgaag caggcgcgga tgtgatgcgt caggacacta
     5341 atatgctgtt tgttcgcgtc ggcgaagaaa atgctgccgc gttaggcgaa tacatgaaag
     5401 cgagaaacgt actgattaac gcctcgccga ttgtccgcct ggtgacccat cttgacgtct
     5461 cgcgcgaaca actggcagaa gtcgccgccc actggcgcgc attcctggcg cgttaaggag
     5521 ggagacgtgc cgcaacgcat tttagttctt ggtgccagtg gctacattgg tcagcatctg
     5581 gtgcgcacac tcagccagca agggcatcag atcctggcgg cggcacgtca tgtcgacagg
     5641 cttgcaaagc tgcaactggc aaacgtcagt tgccataaag tcgatctcag ctggccggat
     5701 aaccttccgg ccctgttaca ggatatcgat acggtctatt ttctggtgca tagcatgggc
     5761 gaaggcggcg attttatcgc tcaggagcgc caggtggctc tcaacttccg cgatgcgcta
     5821 cgtgaagtac cagttaagca attaatcttt ctcagttcgt tgcaggcccc gccacatgag
     5881 cagtcggacc atctgcgcgc gcgtcaggct acggcggaca ttcttcgtga agcgaatgta
     5941 ccagtgaccg aattgcgtgc cggaataatc gttggtgcag gttcagcagc gttcgaagtc
     6001 atgcgcgata tggtctacaa cctgccagtg ttaacgccgc cacgctgggt acgttcacgc
     6061 accacgccca tcgcgctgga aaacttgctg cactatctgg tggcgctgtt agaccatcca
     6121 gccaacgaac accgcatctt cgaagccgcc ggaccagagg tgctcagtta tcagcaacag
     6181 tttgaacatt ttatggcggt gagcggtaag cgccgctggt tgatccccat ccccctcccc
//
LOCUS       DBFIFS010000243         6124 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.243, whole genome shotgun sequence.
ACCESSION   DBFIFS010000243 DBFIFS010000000
VERSION     DBFIFS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.243"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            21..1241
                     /gene="astC"
                     /locus_tag="N7A07_004195"
     CDS             21..1241
                     /gene="astC"
                     /locus_tag="N7A07_004195"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU2478744.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            1238..2272
                     /gene="astA"
                     /locus_tag="N7A07_004196"
     CDS             1238..2272
                     /gene="astA"
                     /locus_tag="N7A07_004196"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU2478745.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2269..3747
                     /gene="astD"
                     /locus_tag="N7A07_004197"
     CDS             2269..3747
                     /gene="astD"
                     /locus_tag="N7A07_004197"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU2478746.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVLGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            3744..5087
                     /gene="astB"
                     /locus_tag="N7A07_004198"
     CDS             3744..5087
                     /gene="astB"
                     /locus_tag="N7A07_004198"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU2478747.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            5080..6048
                     /gene="astE"
                     /locus_tag="N7A07_004199"
     CDS             5080..6048
                     /gene="astE"
                     /locus_tag="N7A07_004199"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU2478748.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1306 a         1545 c         1842 g         1431 t
ORIGIN      
        1 tatttactgt agaggtcgct atgtctcagc caattacgcg tgaaaacttt gatgaatgga
       61 tgatacctgt ttacgctccg gcaccgttta taccggtacg tggcgaaggt tcgcgcttgt
      121 gggatcagca ggggaaagag tatatcgact tcgcgggtgg cattgcggtg aacgcgctgg
      181 gccatgcgca tccggaactg cgtgaggcgc tgaacgaaca ggcgagtaag ttctggcata
      241 ccggcaacgg ttacaccaac gagtcggtac tgcgactggc gaaaaaattg atcgacgcca
      301 cgtttgccga tcgcgttttc ttttgtaact ccggtgcgga agccaacgaa gcggcgctaa
      361 aactggcgcg taaattcgct cacgaccgct acggcagcca taagagcggt atcgtggcgt
      421 ttaaaaatgc gtttcatggt cgcacgctgt ttactgttag tgcgggtggg cagccagcct
      481 attcacagga ttttgcgcca ctgccaccag atatccgtca tgctgcatat aacgatatta
      541 actctgccag cgcgctgatt gacgactcta cctgtgcggt gattgtcgaa cccatccagg
      601 gtgaaggcgg tgtggtgcca gccagcaacg catttttaca aggtctgcgt gaattgtgtg
      661 atcgccacaa tgcgctgttg atttttgatg aagtacaaac cggcgtcggg cgtaccgggg
      721 aactgtatgc ctatatgcac tacggcgtga cgcctgatct gttaactacc gccaaagctc
      781 tgggcggcgg tttcccggtc ggtgcgttgt tagcaaccga agagtgcgcc agcgtgatga
      841 ccgttggcac tcatggcacc acctatggcg gcaatccgct ggcctcggcg gtggcaggca
      901 aagtgctgga gctcatcaat acgccagaga tgcttaacgg ggttaagcaa cgtcacgact
      961 ggtttgttga gcgtcttaat attattaatc accgctatgg tttgttcaat gaagttcgcg
     1021 gcttaggttt gctgattggc tgtgtactga atgccgatta cgccgggcaa gcgaaacaga
     1081 tctctcagga agcggcgaaa gcaggcgtga tggtactgat tgcgggtggc aacgtggtgc
     1141 gttttgcgcc tgcgctcaat gtcagcgaag aagaggtgac gaccggactg gatcgctttg
     1201 cagttgcttg cgaacacttt gttagccgag gttcatcatg atggtcatcc gtcccgttga
     1261 gcgctcagat gtctcggcgc tgatgcagct tgccagcaaa acgggcggcg gcctgacgtc
     1321 gcttcccgcc aatgaagcca cgctttcggc gcgtatcgaa agggcaatca aaacctggca
     1381 aggtgaactg cccaaaagtg agcagggtta tgtgtttgta ctggaagata gcgagacagg
     1441 caccgtggcg gggatttgcg ccattgaggt ggcagttggg ctgaacgatc cctggtacaa
     1501 ctatcgcgtc ggcacgttgg ttcacgcctc aaaagagctg aatgtctata acgcattgcc
     1561 gacgttgttt ctcagtaacg atcacaccgg cagcagcgag ctgtgcacgc tgtttctcga
     1621 cccggactgg cgcaaagagg gcaacggcta tttgctgtcg aaatcgcgct ttatgtttat
     1681 ggcggctttt cgcgacaagt ttaatgacaa agtggttgct gaaatgcgcg gggtgattga
     1741 cgaacacggc tattcaccgt tctggcaaag cctcggtaaa cgcttctttt cgatggattt
     1801 tagccgcgcc gattttctct gcggcaccgg gcaaaaggca tttattgcag aactgatgcc
     1861 gaaacatccg atctatacgc actttttatc ccaggaagcc caggacgtca tcggtcaggt
     1921 acatccgcaa accgcgcctg cccgcgcggt gctggagaaa gaaggttttc gctaccgcaa
     1981 ctatatcgac atcttcgacg gtgggccgac gcttgagtgt gacatcgacc gcgtgcgcgc
     2041 catccgtaaa agtcggctgg tggaagtggc agaagggcag cctgcgcagg gcgattttcc
     2101 ggcctgcctg gtcgccaatg aaaattatca ccatttccgc gtggtgctgg tgcgtaccga
     2161 tccggcaacc gagcgtttga ttttaaccgc cgcacaactg gatgccctca aatgccatgc
     2221 aggggatcgc gttcgtctgg tgcgcctgtg cgcagaggag aaaacagcat gactttatgg
     2281 attaacggtg actggataac gggccagggt gcatcgcgtg tgaagcgtaa tccggtatcg
     2341 ggcgaggtgt tatggcaagg caatgatgcc gatgccgctc aggtcgggca ggcttgtcgg
     2401 gcagcccgtg cggcgtttcc gcgctgggcg cggctctcat ttgctgaacg tcaggccgtt
     2461 gtcgaacgct ttgccggatt gctggaaagg aataaaggcg aattaaccgc aatcattgcc
     2521 cgagaaaccg gcaagccgcg ctgggaggcg gcaaccgaat tgacggcgat gatcaataaa
     2581 atcgcgatat caattaaggc gtatcacgtt cgtaccggcg agcagcgtag tgaaatgccg
     2641 gacggtgcgg cgagcctgcg acatcgcccg cacggcgtgc tggcggtgtt tgggccgtat
     2701 aatttccctg gtcatttgcc gaacggacat atcgttcccg cattgctggc aggtaacacc
     2761 attatcttta aacccagcga actgacaccg tggagtggcg aagcggtaat gcgtttatgg
     2821 cagcaggctg gcttgccgcc aggcgtgctg aacctggtgc tgggcgggtg tgaaacgggt
     2881 caggcgctga gtgcgctgga ggatctcgac ggcttgctgt ttaccggcag cgccaatacc
     2941 ggctaccagc tgcatcgcca gctctccggt cagccggaga aaattctcgc ccttgagatg
     3001 ggcggcaata acccgctgat tatcgatgag gtggcggata tcgacgcggc tgtccatctg
     3061 accattcagt cggcgtttgt tacagccggg caacgctgca cctgcgcccg ccgtgtattg
     3121 ctgaaaagcg gggcgcaggg cgatgcattt cttgctcgtc tggttgccgt cagccagcga
     3181 ttaacgccgg gcaactggga tgacgaaccg cagccgttta ttggcgggct gatttctgaa
     3241 caggccgcac agcaggtgtt tactgcctgg cagcaactgg aagcgatggg tggacgaacc
     3301 ctgcttgcgc cgcgcttatt acaagcaggg acatcgttgc tgacgccggg gatcattgaa
     3361 atgacaggcg ttgctggcgt accagatgaa gaggtgttcg gaccgttatt gcgcgtctgg
     3421 cgttatgatt ctttcgagga agcgattcga atggcgaata acactcgctt cggactctct
     3481 tgcggtctgg tttcccccga gcgggaaaaa ttcgatcaac tgttgctgga ggcgcgggcg
     3541 gggattgtta actggaacaa accgcttact ggtgctgcca gtaccgcgcc attcggcggc
     3601 attggtgcat ccggtaacca tcgccccagc gcctggtatg ccgcagatta ctgcgcatgg
     3661 cctatggcga gcctggagtc ggactcgtta actttgcccg caacgcttaa ccccgggctg
     3721 gatttttccg atgaggtggt gcgatgaacg cctgggaagt taatttcgac gggctggtag
     3781 ggctgacgca tcattacgcg ggcctgtcgt ttggtaataa agcctctacc cgtcaccgtt
     3841 ttcaggtctc taacccgcga ctggcggcaa aacagggctt actgaaaatg aaaacccttg
     3901 ccgatgcggg attcccccag gccgtgatcc cgcctcacga gcgtccgttt attccggtgc
     3961 tgcgtcagtt gggattcagt ggtagcgatg agcaggtact ggaaaaggtt gtacgccagg
     4021 caccgcactg gctttccagc gtcagttccg cttcgccaat gtgggtagcc aatgcggcaa
     4081 cgatcgcgcc atctgccgat acgctggatg gcaaagtgca tctcaccgtt gctaacctga
     4141 acaataaatt tcaccgttcg ctggaagcgc ccgtcactga atcgctgtta aaagcgattt
     4201 ttaacgacga agagaaattt agtgtccatt cggcgttgcc gcaggtagcc ttgctcggtg
     4261 atgagggggc ggcaaaccac aatcgtctcg gcggtcatta cggtgaaccg ggtatgcaac
     4321 tttttgtcta cgggcgagaa gagggcaatg atacccggcc ttcccgttat ccggcgcgac
     4381 agactcgcga agccagcgag gcggtggcaa ggctgaatca ggtgaatccc caacaggtga
     4441 ttttcgccca gcaaaacccg gacgttatcg accagggcgt ttttcataat gacgtgattg
     4501 ccgtgagtaa ccgccaggtg ctgttttgcc accaacaggc gttcgctcgc cagtcacagt
     4561 tactggcaaa cctgcgtgcg cgggtgaacg gttttatggc gatagaagtt ccggcaactc
     4621 aggtttccgt gtcggatgcg gtgtctacat atctgtttaa cagccaactg ttgagccgcg
     4681 atgatggttc catgatgttg gtgctgcctc aggagtgtcg ggaacacgcc ggagtatggg
     4741 gttatctcaa tgaactcctt gccgctgaca acccgattag cgaactaaaa gtctttgatt
     4801 tacgtgaaag catggcgaat ggcggtggcc cggcgtgcct gcggttgcgg gtggtattga
     4861 cacaagaaga acgccgggcg gtgaatccgg cggtgatgat gaacgatacc ttgtttaatg
     4921 cgctcaatga ctgggtggat cgttactacc gcgatcgtct tactgctgcc gatctggccg
     4981 acccgcaatt gctgcgcgaa gggcgggaag cactggatgt attgagccaa ttactgaatc
     5041 tcggttcggt ttatccgttc cagcgcgagg gagggggcaa tggataattt tcttgctctg
     5101 accttaacga gtaaaaaacc tgtcatcacc gagcgagaaa tcaacggcgt tcgctggcgc
     5161 tggctgggcg atggtgtgct ggaactgacg ccattaacgc caccgcaagg cgctctggtg
     5221 atttctgcgg ggatccacgg taatgagacg gcacctgtgg agatgcttga tgcattgctt
     5281 ggcgcgatat ctcacggcga gatcccgtta cggtggcggt tgctggtgat cctcgggaat
     5341 cctcctgcgc tgaagcaagg gaaacgttat tgccatagcg atatgaaccg aatgtttggc
     5401 ggtcgttggc agctgtttgc tgaaagcggg gaaacctgtc gggcgcgcga actggaacag
     5461 tgcctggaag atttttatga ccagggcaaa gaatctgtgc gctggcacct tgatctacat
     5521 accgcaattc gtggctcctt gcatccgcag ttcggtgtat taccgcaacg cgacattccc
     5581 tgggacgaga aattcctgac gtggctgggt gcggcggggc tggaggcgct ggtgttccat
     5641 caggagcctg gtggtacgtt tacccatttc agcgccagac attttggcgc gctggcctgt
     5701 acgctggaac ttggcaaagc gttgcccttt gggcaaaacg atcttcgcca gtttgcagta
     5761 actgccagcg caatcgctgc gctgctatct ggtgagagtg tcggtatcgt gagaacaccg
     5821 ccgctccgtt atcgggtggt ttcgcaaatt actcgccact cgccgtcctt cgaaatgcat
     5881 atggcaagtg acacgctgaa ttttatgccg tttaagaaag gaacgttgct ggcgcaggac
     5941 ggtgaggaac gttttaccgt aacccatgat gtagagtatg tgttattccc taatccgttg
     6001 gtagcgctgg gattacgcgc gggattaatg ctcgaaaaaa taagctaata cttacccgca
     6061 gaaatctttc tgcgggtatt aagttacctt cttataaatt aatacagatt aatcctggtt
     6121 attt
//
LOCUS       DBFIFS010000244         6061 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.244, whole genome shotgun sequence.
ACCESSION   DBFIFS010000244 DBFIFS010000000
VERSION     DBFIFS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.244"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            106..1161
                     /gene="phoE"
                     /locus_tag="N7A07_004200"
     CDS             106..1161
                     /gene="phoE"
                     /locus_tag="N7A07_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU2478749.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1200..1601)
                     /gene="crl"
                     /locus_tag="N7A07_004201"
     CDS             complement(1200..1601)
                     /gene="crl"
                     /locus_tag="N7A07_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU2478750.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1659..2903)
                     /gene="frsA"
                     /locus_tag="N7A07_004202"
     CDS             complement(1659..2903)
                     /gene="frsA"
                     /locus_tag="N7A07_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU2478751.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2995..3453)
                     /gene="gpt"
                     /locus_tag="N7A07_004203"
     CDS             complement(2995..3453)
                     /gene="gpt"
                     /locus_tag="N7A07_004203"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2478752.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3714..5171
                     /gene="pepD"
                     /locus_tag="N7A07_004204"
     CDS             3714..5171
                     /gene="pepD"
                     /locus_tag="N7A07_004204"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU2478753.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5228..5749)
                     /gene="prfH"
                     /locus_tag="N7A07_004205"
                     /pseudo
     CDS             complement(5228..5749)
                     /gene="prfH"
                     /locus_tag="N7A07_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5748..5951)
                     /locus_tag="N7A07_004206"
                     /pseudo
     CDS             complement(5748..5951)
                     /locus_tag="N7A07_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
BASE COUNT         1546 a         1539 c         1498 g         1478 t
ORIGIN      
        1 attaatctgt aatatatctt taacaatctc aggttaaaaa ctttcctgtt ttcaacgggg
       61 ctctcccgca gaatattcgc gcgttaatta aaatcaggaa tgaaaatgaa aaagagcact
      121 ctggcattag tggtgatggg cattgtggca tctgcatccg tacaggccgc agaaatatat
      181 aacaaagacg gtaataaact ggatgtctat ggcaaagtta aagccatgca ttatatgagt
      241 gataacgaca gtaaagatgg cgaccagagt tatatccgtt ttggttttaa aggcgaaaca
      301 caaattaacg atcaactgac tggttatggt cgttgggaag cggagtttgc cggaaataaa
      361 gcggagagtg atactgcaca gcaaaaaacg cgtctcgctt ttgccggatt gaagtataaa
      421 gatttgggtt ctttcgacta tggccgtaac ctgggcgcgt tgtatgacgt ggaagcctgg
      481 accgatatgt tcccggaatt tggtggcgac tcctcggcgc agaccgacaa ctttatgacc
      541 aaacgcgcca gcggtctggc gacgtatcgg aacaccgact tcttcggcgt tatcgatggc
      601 ctgaacttaa ccctgcaata tcaagggaaa aacgaaaacc gcgacgttaa aaagcaaaac
      661 ggcgatggct tcggcacgtc attgacatat gactttggcg gcagcgattt cgccattagt
      721 ggggcctata ccaactcaga tcgcaccaac gagcagaacc tgcaaagccg tggcacaggc
      781 aagcgtgcag aagcttgggc tacaggtctg aaatacgatg ccaataatat ttatctggca
      841 actttttatt ctgaaacacg caaaatgacg ccaataactg gcggctttgc caataagaca
      901 cagaactttg aagcggtcgc tcaataccag tttgactttg gtctgcgtcc atcgctgggt
      961 tatgtcttat cgaaagggaa agatattgaa ggtatcggtg atgaagatct ggtcaattat
     1021 atcgatgtcg gggctacata ttatttcaac aaaaatatgt cagcgtttgt tgattataaa
     1081 atcaaccaac tggatagcga taacaaattg aatattaata atgatgatat tgtcgcggtt
     1141 ggcatgacct atcagtttta atgattattg ccggatgtga tgcatccggc acatttcact
     1201 cacgccgtta acttcaccgg ctcgtcacga aaatcatccg ccggttccaa cttcagattc
     1261 agcgtcgtca gcagttcacg cagcttctcg tgaaactcac gcagggtgtg ctccagtcgt
     1321 tcaaccactt cagtatcttt taccggaaca ctcttccagt cgcctgcttt atcgaacaga
     1381 ccaaactggt aactgtaggt aaaacgggat tcctgcgctt caagctccat ccaccagccc
     1441 cagaactcac gcacttccgg tgccggtttc acgttgacgc atacagccag acaatcgaaa
     1501 aagaatcgat tatcttcgca cttaccttca cgaatatacg ggcctagtac ggtaaatttt
     1561 ttgatcaatc tgctcttcgg gtgtccactc ggtaacgtca ttgctatctc ctgttgtgat
     1621 gcaactgttt taccaaattg gcaaaattta gcaaattttt aacacaagcg tttttcgatc
     1681 cagtcggtga tttcctgaag acctttgtca aaattccgat acaccgggtt aaatgggatc
     1741 tctaataatt taccgtcagc agatgatgag gtgattaagc gtgagtcctc ttccgggctg
     1801 aacggatcgt tcttccagta gcctgataac attggcgttg ggcatcgacg tcccagcaat
     1861 ccttgcactt ttaatgaata gcgattcagc tccacgcgca acgcttcgtc ggaagcatca
     1921 tgcatcccca aacgactcgc cagaacgtca agatacattt ccggcacctg ttgctggcac
     1981 ttaaaatcac tcaacagggt atgaactacc ggaccaagac aggcaaccgc tttcagacgc
     2041 ggcgattcaa gatatgccag acgcacggca acgttagcgc cgaaacggaa accaaaggcc
     2101 gcgacgcgag tgtgatccac ccacggtacg ttaggcagag cctttaagac gcgctgatgc
     2161 aacaggcagg agtcctgggt gagcttccat tttgaagaaa agcccaccga cggcatatca
     2221 atagtcagca tcgcaatgcc gcgcggcgca aaataacgtt catacaggct gtaatagtcc
     2281 gtctgcatcg catccagacc accacacatt aataccgtcg ggaacgggcc atcgcctttc
     2341 ggcatatgca aaaagccggt gatgggcgca ccgccgggta cggtaaactc catctgtcgc
     2401 atcgtgcccg gtagacgctg agcggcctct tcataggcgc ggtttgacaa agcctgcgct
     2461 tgctcggcca ggtcatctcc tttcagatga ggataggcgg caatgttgta caacgtagcc
     2521 gcatgcagcc agtgacgacc actgagttgc ggatcttctt ccgcacaggc tttttgttgc
     2581 cacaccatcg cctgggtggc ccactcataa atccagttgc cgccacggta gccaatcacc
     2641 gtatcgtata aatcatcgtc ggtacgttcg gcatcgctca tcacaatacg tgcctggacg
     2701 tcgaggattt cgcgtgggtc aatgccgcgc cagatccaca tcagacggtt aatcatgcga
     2761 taccagtgag ggatggtttt accatcaagg gccgactgca caggcggttg tgcgccgtga
     2821 ttaaagcggc ggactagcgt cgaggtttca ggatgtttaa agcggggttt gaacagggtt
     2881 tcgctcaggt ttgcctgtgt catggatgca gcctccagaa tacttactgg aaactattgt
     2941 aactcgcctg aagttaaaaa gaacaacgcc cggcagtgcc aggcgttgaa aagattagcg
     3001 accggagatt ggcgggacga atacgacgcc catatcccac ggctgttcga tccaggtatc
     3061 ttgcgggata tcaacaacat aatcatcaac cagcggacga ccagccggtt ttgcaaagat
     3121 ggtgacaaag tgcgcttttg gatacatttc acgaatcgca accgcagtac caccggtatc
     3181 caccaggtca tcaataacga tgaagccttc gccatcgcct tctgcgcgtt tcagcacttt
     3241 aagctcgcgc tggttgtcgt gatcgtagct ggaaatacaa acggtatcga catgacgaat
     3301 acccagttca cgcgccagta acgcacccgg taccagaccg ccacggctta cggcaataat
     3361 gcctttccat tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa
     3421 catgtcccag gtgacgatgt atttttcgct catgtgaagt gtcccagcct gtttatctac
     3481 ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact
     3541 aaaaaccagt atttcacatg agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg
     3601 ataaagtggt attctcaaac atatctcgca agcctgtctt gtgttgacaa cattttctgc
     3661 taaccctgtg acctgcaata ctgttttgcg ggtgatcgac aaggagactt aacgtgtctg
     3721 aactgtctca attatctcca cagccgctgt gggatatttt tgccaaaatc tgttctattc
     3781 ctcacccgtc ctatcatgaa gagcaactcg ctgaatacat tgttggttgg gcaaaagaga
     3841 aaggtttcca tgtcgaacgc gatcaggtag gtaatatcct gattcgtaaa cctgctaccg
     3901 caggtatgga aaatcgtaaa ccggtcgtct tgcaggccca cctcgatatg gtgccgcaga
     3961 aaaataacga caccgtgcat gacttcacga aagatcctat ccagccatat attgatggcg
     4021 aatgggttaa agcgcgcggc accacgctgg gtgcggataa cggcattggt atggcctctg
     4081 cactggcggt tctggctgac gaaaacgtgg ttcacggccc gctggaagtg ctgctgacca
     4141 tgaccgaaga agccggtatg gacggtgcgt tcggcttaca gagcaactgg ttgcaggctg
     4201 atattctgat taacaccgac tccgaagaag aaggtgaaat ctacatgggt tgcgcggggg
     4261 gtatcgactt cacctccaac ctgcatttag atcgtgaagc ggttccagct ggttttgaaa
     4321 ccttcaagtt aaccttaaaa ggtctgaaag gcggtcactc cggcggggaa atccacgttg
     4381 gcctgggtaa tgccaacaaa ctgctggtgc gcttcctggc gggtcatgca gaagaactgg
     4441 acctgcgtct tattgatttc aacggcggca cactgcgtaa cgccatcccg cgtgaagcct
     4501 ttgcgaccat tgctgtcgca gctgataaag tcgacgccct gaaatctctg gtgaatacct
     4561 atcaggagat cctgaaaaac gagctggcag agaaagagaa aaatctggcc ttgttgctgg
     4621 actctgtagc gaacgataaa gctgccctga ttgcgaaatc tcgcgatacc tttattcgtc
     4681 tgctgaacgc caccccgaac ggtgtgatcc gcaactccga cgtggcaaaa ggtgtggtcg
     4741 aaacctccct gaacgtcggt gtggtgacca tgactgacaa taacgtagaa attcactgtc
     4801 tgatccgttc tctgatcgac agcggtaaag actacgtggt gagcatgctg gattcgctgg
     4861 gtaaactggc tggcgcgaaa accgaagcga aaggcgcata tcctggctgg cagccggacg
     4921 ctaattctcc ggtgatgcat ctggtacgtg aaacctatca gcgcctgttc aacaagacgc
     4981 cgaacatcca gattatccac gcgggcctgg aatgtggtct gttcaaaaaa ccgtatccgg
     5041 aaatggacat ggtttctatc gggccaacaa tcaccggtcc acactctccg gatgagcaag
     5101 ttcacatcga aagcgtaggt cattactgga cactgctgac tgaactgctg aaagaaattc
     5161 cggcgaagta attatttgat ttgctgccgg atggcgttta atcgccttcc ggcagtttca
     5221 tccttcatta tccttcgata aaagccatcc ctgtgaacgt tcgtcgcggg ttgccacgtt
     5281 caatctggtg atggaacatt cgccgctgcg atttcagcac tgcgctattt tcctgttgct
     5341 gctgctccag cttccaggca atcagcaatc gcgccagccg tttgttagca tgctggctgc
     5401 gttctgactg aaccttcacg ctaatcccgg ttgccagatg cgtggcgcgt accgccgagt
     5461 cggttttatt gacatgttga ccgcccggcc ccgacggacg cagcgtctca taacggattg
     5521 catccgattg ttcctgctca tcagcggtaa aacgcccaat gcccagaaac cagtttttgc
     5581 gcccatgatg aggccgatac ggactcagac aaatccactg aatagtgccg caccaacttt
     5641 cgcttaacgc ccatgcgttg tcgccatcca gagaaatcag cgccgaacgc agcgtgtcag
     5701 agtagcggcc cgtttctgtt tccagcaccg ttaccgcgac gtcttgtcgg gtaaaaacga
     5761 ccaacagaga agaacgctgc gccaatcggg tagccgcgtc cggggtgtaa atctggcatc
     5821 cccactacgc gctgcatgtt gggtaaattt gccgtggtgt gtaattgttg gatcgctaaa
     5881 ctctcgatcc acaggtcatc agatgcgatg gtaaataccg catcggataa gggacgaata
     5941 tatttgccca tgtaccattc catttatact cgtcatactt caagttgcat gtactgcgac
     6001 tgcgttcgtt caccccagtc acttacttat gtaagctcct ggggattcac tcgcttgtcg
     6061 c
//
LOCUS       DBFIFS010000245         6012 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.245, whole genome shotgun sequence.
ACCESSION   DBFIFS010000245 DBFIFS010000000
VERSION     DBFIFS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.245"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..203)
                     /locus_tag="N7A07_004207"
     CDS             complement(<1..203)
                     /locus_tag="N7A07_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2478754.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(268..867)
                     /locus_tag="N7A07_004208"
     CDS             complement(268..867)
                     /locus_tag="N7A07_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2478755.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(935..1657)
                     /locus_tag="N7A07_004209"
                     /pseudo
     CDS             complement(935..1657)
                     /locus_tag="N7A07_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            1645..2061
                     /locus_tag="N7A07_004210"
                     /pseudo
     CDS             1645..2061
                     /locus_tag="N7A07_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            2189..2725
                     /gene="yfdR"
                     /locus_tag="N7A07_004211"
     CDS             2189..2725
                     /gene="yfdR"
                     /locus_tag="N7A07_004211"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU2478756.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            2716..3066
                     /locus_tag="N7A07_004212"
     CDS             2716..3066
                     /locus_tag="N7A07_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HGU2478757.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            3063..3536
                     /locus_tag="N7A07_004213"
                     /pseudo
     CDS             3063..3536
                     /locus_tag="N7A07_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            3683..3880
                     /locus_tag="N7A07_004214"
     CDS             3683..3880
                     /locus_tag="N7A07_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478758.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            3838..4188
                     /locus_tag="N7A07_004215"
                     /pseudo
     CDS             3838..4188
                     /locus_tag="N7A07_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4225..4506
                     /locus_tag="N7A07_004216"
     CDS             4225..4506
                     /locus_tag="N7A07_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HGU2478759.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(4554..4727)
                     /locus_tag="N7A07_004217"
     CDS             complement(4554..4727)
                     /locus_tag="N7A07_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478760.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(4924..5457)
                     /locus_tag="N7A07_004218"
     CDS             complement(4924..5457)
                     /locus_tag="N7A07_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478761.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(5604..5708)
                     /locus_tag="N7A07_004219"
                     /pseudo
     CDS             complement(5604..5708)
                     /locus_tag="N7A07_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         1673 a         1444 c         1360 g         1535 t
ORIGIN      
        1 gggaatcctt ccaccaacag aatgacgctg tactgaccgt actcaacgtc catgctgtaa
       61 cgaccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtc
      121 cgtctggctt tcagttgaat ggtgcagttc tctaccggtt ttcctgtgcc gtctttcagt
      181 acacctgaaa tctttactgc catattcacc ccacaaaaaa gcccgcctga accggcgggc
      241 tgtcataaca ctgtgttacc tggctaatca gaatttatag ccgacaccca cgatgaagcc
      301 gtcagtgcgc cagtcgccac tgccggagcc ttcataagca atatcaatgg ccacggattc
      361 ggtcgggtta aactgcacgc cagctcccca cgccagagac gtgttgctgt ggcgaccgtc
      421 atcacttccg gtcagcacat catgcgtttt ccccttgttg tcagttacgc ggagataatc
      481 cccggagaaa gtcgacacac ggctgtaagc cacacccgcc atcgcatacg cgctgaacca
      541 ttcattcacg cgcacagagg gccccgccat cacgctgaac cagcggttac gcacggaatc
      601 ttcatgccag cgggtatcgc tgtaacgggt aagctggcga ttcctgtctc ctgcatagct
      661 gaatgacgtc accatcccca gtgtgtccgt aaactcataa cggtatttca cgttaatccc
      721 gttaagatta tcgctaccgg gagcgctcgt ccgggcatga agataccccg cgctcagcgt
      781 ggcctgctgc tcagacgccc atgcaggcgc gccggatacg gccagacaga tggctgcgga
      841 caaaatggcg gcataaagtt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc
      901 gccatttctg gcgcccgtat atgggttata aaattcagct gatactgatg cctgcggtgg
      961 ctttcttcat caccacaacc agcaaatcgc tgatacttgc tgtgggatac cagtcattta
     1021 ccagccatgc tgacaccgaa aactccagcg tcatgtgacc gtgaccggca ggcatatcaa
     1081 taacgccact gtaaatcagc gtattatcca gcgcggtacg gttataaatt tcagcaccgt
     1141 ttttccgcac tatcagacgg catgaggagt aaatatcagt atgctctctc tcatgcttag
     1201 cgccactgaa tgccaccgcc ggaataacaa tctgccggtc aaacggctga tcgtcataaa
     1261 ccctgacggt aatggtccct gatggccacc gttccggtgc acgggagtcc cgggggaaag
     1321 ctttgcccac tgttttaacg agatcgcctt caatctggtt cgcggacagt tttcccagaa
     1381 cccgacagtt ctcgttaatc gtgacgttgt tgagcgtccc ggagttcgca ttcacgttac
     1441 cactgatatc cgcattttta gcagtcagct ttccatccgg tgtcagggaa aatgccggtg
     1501 gatttccacc actggtaatg gtgggggccg tcaggcgctt caggaacacg tcgttcatga
     1561 atatctggtt gccctgcgcc acaaacatcg gcgtttcatt cccgtttgcc gggtcaataa
     1621 acgcgatacg attggcggca accagaatgg attgaagact gggccgacta ccttgtgggc
     1681 tttgatgcta atggtgacac cattcaggca acaaaagcgg ctgcggcggt ccgtaaaatc
     1741 acgattgaag caaaccagac cgctgatttt gaagataatg acttcagcgg caaacgctcc
     1801 ctgatggagt ctgtcgaagc gaagaccaaa gacattatgc cagtggcatt tgaatttaaa
     1861 tgcattccgt ttgaaggtct gaaagaacgt ccgtttaaat tacgcctcag cattatcact
     1921 ggcgatcgtc cggtactggt tctgcgcatt attcagctgg aggcggtgca ggaagaaatg
     1981 gctaacgaat ttcgtgatct gcttgttgag aaattcaagg acagcaaagt agaaaccttt
     2041 attggtactt tcaccgcctg atttcattac tgcaaatgcc cctgaggggg catttatgga
     2101 aacgtaattt actcaataat cgccggatgg tgagggattc tttttaccag aattcagcgc
     2161 ggtgcagcgc atatacgtgg agaacaaaat gtcatttatt aaaacttttt ccgggaagca
     2221 tttttattat gacaggataa ataaagacga catcgatatt aacgatatcg cggtttccct
     2281 ttcaaatatc tgtcgctttg ccggtcatct ttcgcacttc tacagcgtcg cccaacatgc
     2341 ggttctttgc agccagctgg taccgcagga atttgctttt gaagcattaa tgcatgatgc
     2401 aacagaagcg tattgccagg acatccccgc tccactgaaa cgccttcttc ctgactataa
     2461 acggatggaa gaaaaaattg acgccgtaat ccgtgagaaa tacgggttac ccccagttat
     2521 gagtacgccc gtgaaatatg ccgatcttat catgctggca accgaacgcc gcgatctcgg
     2581 gcttgatgat ggctctttct ggcctgtact ggaaggcatc ccggcaacag agatgttcaa
     2641 cgtgattcca ctggcaccta gccatgccta cgggatgttt atggaacgtt ttaacgagtt
     2701 atcggagtta cacaaatgcg catgaatgtt ttcgaaatgg aagggtttct tcgcgggaaa
     2761 tgtgtaccgc gagatctgaa agtgaatgaa acaaatgctg agtatctggt gcgtaaattc
     2821 gatgaagtac gtgctgaggc tcgcaacgag ggtattaact ataccgcaag ccgtcttgct
     2881 gctgctttca atcacggatt tatcaataag cctttggctg aagttttcga cgttacacgc
     2941 atgattctgt cagcaaaaga agagttagct aatgaatcgc atccgattga tggcctgtcc
     3001 ggtgaatatg cagagaaatc ccttgaagaa tgggcggaac ggcttcgcaa aggaggcagc
     3061 cagtgactgg acatgcagca atcctcgaca tgtgctgtgg cagtcgcatg ttctggttcg
     3121 ataagaatga cgaccgggcg atatttagcg atatcagaaa ggaagagcac acattgtgtg
     3181 atggacgacg cctgattatc agtcctgatc tgatagctga ttttcgtgca ctaccatttg
     3241 cagacgcatc tttttcgatg gttatattcg accctccgca tcttgagcgt gttggtgata
     3301 acgcctggat gggaaagaaa tatggacggc tgaataaaga tacctagcgt gatgatttgc
     3361 ggcagggatt taaagaagcc tttcgtgtgt tgcgtccatc cggcgttctg atttttaaat
     3421 ggaatgaaac gcaaatacct gttcgccaga tattggtact gaccgacaga aaacctgtta
     3481 tcggtcaacg aacaggaaaa aacgataaaa cccactggat tatttttatg aaataggcat
     3541 ccagtgagta ggttcgtaag gttacagata cgtatatctg aataattaaa ttcagttctg
     3601 taaataaaat ttaatcctta accggaggga ttcctgcacc ctcagaacat caggagaccg
     3661 cccgaaaggg cggtaatgaa aaatggctga attaaccaaa tggctacaaa acacgattac
     3721 cggaattgaa acggtagtag acgataaatc gtttgtatgt gatgaaatag tattcaaaat
     3781 cgatgtggtt aaaaacgtac ttaccgcatt taaagtcgcg ctggtatcgc tggaagtatt
     3841 gatgatactt tgtcgatcag cgatgctctc tcagaactaa accatttcat taaccggcac
     3901 gcagacaata cgaaatattt aaaagtctgg ggtaacgggg ccaccttcga caacgtaatt
     3961 ttacgtggag cttacgagcg agcaggacaa atctgcccgt gggcgtactg gaatgaccac
     4021 gatgtacgca cgatcgttac gcttgggcgt tccatcggat tcgaccccaa aatggacatg
     4081 cctttcgatg gcgaacggca caacgccctg gctgatgccc gtcatcaggc aaaatatgtt
     4141 tccgctatct ggcagaaatt aattcctgcc accagcacag aattatgatt ttcccgggtg
     4201 cagccggttt tgatggagaa aattatgaac accttgtttt tactgatggc tgaattcaat
     4261 accccaaaca ttgaactctc agcagttagc caaaagtact ttggtatgag tccagccacg
     4321 gcagaagcaa aagcaaacgc ttgtaagttg cccgttccaa catatcgcat cggcacatca
     4381 caaaaagcaa aacgttgcat caatattcaa gatcttgcgg aatacataga caaaaggcga
     4441 gaagaaggac gtatcgagtg ggaacaggtc agaacagtca aacagaagga caaagaacat
     4501 cactaaagaa aaaacccgcc tgaaggcggg ttttcaaaaa gcaccagcta tgatcatgct
     4561 gctttgcgac gacgaagctt accctgctgc tctttaccag agacagtagc gtgagtgaac
     4621 gcattaggag cagccttcat cagaacttca acagcagcac ccatacctgc gaatgctttc
     4681 attgtgtcga acttaacctg tggcttggtt gctttttgat ctttcataga aaactcccga
     4741 gacagtaaag gcgtctctaa ccctttcttt aaagctagct tgtttcgcta acttatgcca
     4801 atcgatcatg tcgattggtg acatcgtttc ttagtagttt aagcacaaaa cgactgccat
     4861 agatgtacct ttaaggtaat ctggacgggt atcctacaat ttgtagaccc ttctcgtcta
     4921 tacctactga gcaaatttaa gaaagatatc ctgcagctca tcaatgactg ccgacatcac
     4981 ataaccgcac tgttccatgc ggaaaccaaa agactcgtaa tactgcacca gttctggtac
     5041 tggctctaca atgtggacaa ctttacattc aacagcttta caaaatataa aagcactcat
     5101 aagagtgagt aaaaccatgc gccctttcaa tgggtgagat tcatcttctc tagaaaacct
     5161 ttcgatcata tggatacgaa agatgttttc ttcaacccca taaacacaaa ttgctgctcc
     5221 tgatggtatt ccctgaaccc gaccttgctg aacaagtttt atgcagaact catacttttc
     5281 tctggagttg ccataggtac ttaacgcata gtcccattca agctcaccat agccaccaca
     5341 cagaatcttg taatcatcat cactgagcgg accaacagca agaggtaagc cgacatgatc
     5401 aataatcaac tggatattgt tacgtactga ttgacctatc tcgtccaggg taagcatcat
     5461 ggcctctcaa gcggaacact aaaagtcgca ttatatctca ttcttaagcc gcgtatggat
     5521 tacaccttga aatgaaaacg ccgggttccc aataggctcc cacaaagtat ataactattt
     5581 gtttttcaaa aacggtacat cctatcgagc attggtgcaa cgctaaaccg accactccag
     5641 tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat ttcagaatta
     5701 ccgtgcattt tcaaatgtag agattatttt atcgatatat catgtggtta tgttattcag
     5761 catcactgtt caggaggctc aataacgggg tactatacca taacaacagg aagcgcctgt
     5821 ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga agtgaatatc
     5881 taatcttatt agatgttagc gttgtgaaaa gcaatgataa acaatacaga ttcgacgata
     5941 aataaaaatc acacattaaa ctccggtgat atctctccct gctaatagca ctgatagaga
     6001 aaaaaagaac cc
//
LOCUS       DBFIFS010000246         5977 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.246, whole genome shotgun sequence.
ACCESSION   DBFIFS010000246 DBFIFS010000000
VERSION     DBFIFS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.246"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            254..901
                     /gene="fucA"
                     /locus_tag="N7A07_004220"
     CDS             254..901
                     /gene="fucA"
                     /locus_tag="N7A07_004220"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU2478762.1"
                     /translation="MERNKLARQIIETCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            929..2077
                     /gene="fucO"
                     /locus_tag="N7A07_004221"
     CDS             929..2077
                     /gene="fucO"
                     /locus_tag="N7A07_004221"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU2478763.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2132..2887)
                     /gene="xni"
                     /locus_tag="N7A07_004222"
     CDS             complement(2132..2887)
                     /gene="xni"
                     /locus_tag="N7A07_004222"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU2478764.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2999..4366)
                     /gene="sdaB"
                     /locus_tag="N7A07_004223"
     CDS             complement(2999..4366)
                     /gene="sdaB"
                     /locus_tag="N7A07_004223"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU2478765.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4424..5713)
                     /gene="sdaC"
                     /locus_tag="N7A07_004224"
     CDS             complement(4424..5713)
                     /gene="sdaC"
                     /locus_tag="N7A07_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU2478766.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1502 a         1476 c         1599 g         1400 t
ORIGIN      
        1 ggcattcctg gcttgggtga atagaaaaat gaccgttttg ataaatattt ttaagagcgg
       61 tcatttttaa tggggttata aaaagagaac ttgcagttat ttactgtgat tagttgaact
      121 aggtcacaaa atataagctt gtgaatttct ttcttgttaa tcaggaagaa gatggtgggt
      181 aattaaacgg ctaattcaat agtgtgaaag gaacaacatt attgccctgt tttgaatcag
      241 agagaggtag ctaatggaac gaaataaact tgctcgtcag attattgaaa cttgcctgga
      301 aatgacccgc ctgggactga accaggggac agcggggaac gtcagtgtac gttatcagga
      361 tgggatgctg attacgccta caggcattcc atatgaaaaa ctgacggagt cgcatattgt
      421 ctttattgat ggcaacggta aacatgagga aggaaagctc ccctcaagcg agtggcgttt
      481 ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg gttgttcaca atcatgccgt
      541 tcattgcaca gcagtttcca ttcttaaccg accgatcccc gctattcact acatgattgc
      601 ggcggctggc ggtaattcta ttccttgcgc gccttatgcg acctttggaa cacgcgaact
      661 ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca actttgttac aacatcatgg
      721 gcttatcgct tgtgaggtga atctggaaaa agcgttatgg ctggcgcatg aagttgaagt
      781 gctggcgcaa ctttacctga cgaccctggc gattacggac ccggtgccag tgctgagcga
      841 tgaagagatt gccgtagtgc tggagaaatt caaaacctat gggttacgga ttgaagagta
      901 atttcgtaaa gcaacaagga gaaggatgat ggctaacaga atgattctga acgaaacggc
      961 atggtttggt cggggtgctg ttggggcttt aaccgatgag gtgaaacgcc gtggttatca
     1021 gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc ggcgtggtgg cgaaagtgac
     1081 cgataagatg gatgctgcag ggctggcatg ggcgatttac gacggcgtag tgcccaaccc
     1141 aacaattact gtcgtcaaag aagggctcgg tgtattccag aatagcggcg cggattacct
     1201 gatcgctatt ggtggtggtt ctccacagga tacttgtaaa gcgattggca ttatcagcaa
     1261 caacccggag tttgccgatg tgcgtagcct ggaaggactt tccccgacca ataaacccag
     1321 tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg gcagaagtga ccattaacta
     1381 cgtgatcact gacgaagaaa aacggcgcaa gtttgtttgc gttgatccgc atgatatccc
     1441 gcaggtggcg tttattgacg ctgacatgat ggatggtatg cctccagcgc tgaaagctgc
     1501 gacgggtgtc gatgcgctca ctcatgctat tgaggggtat attacccgtg gcgcgtgggc
     1561 gctaaccgat gcactgcaca ttaaagcgat tgaaatcatt gctggggcgc tgcgaggatc
     1621 ggttgctggt gataaggatg ccggagaaga aatggcgctc gggcagtatg ttgcgggtat
     1681 gggcttctcg aatgtggggt tagggttggt gcatggtatg gcgcatccac tgggcgcgtt
     1741 ttataacact ccacacggtg ttgcaaacgc catcctgtta ccgcatgtca tgcgttataa
     1801 cgctgacttt accggtgaga agtaccgcga tatcgcgcgc gttatgggcg tgaaagtgga
     1861 aggtatgagc ctggaagagg cgcgtaatgc cgctgttgaa gcggtgtttg ctctcaaccg
     1921 tgatgtcggt attccgccac atttgcgtga tgttggtgta cgcaaggaag acattccggc
     1981 actggcgcag gcggcactgg atgatgtttg taccggtggc aacccgcgtg aagcaacgct
     2041 tgaggatatt gtagagcttt accataccgc ctggtaaatg cgctgatgtg ataatgccgg
     2101 atgcgacgtt tgcggcgtat ccggcacgcc gttaccgtac caaccgcaat tgctgtaaat
     2161 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
     2221 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
     2281 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
     2341 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
     2401 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
     2461 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
     2521 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
     2581 cttcgttgcc gctggttgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
     2641 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg acccgctttg taatctggta
     2701 aacgctgatg acgccagccg ctactgcggt tttcatcgtc aaaaacggcg accgcgtggg
     2761 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
     2821 acccctgaac ggcatgaatg cgacgaataa gattcagtgc atcgacaata agcaaatgaa
     2881 cagccacgat aaccccctta aagatcaggc gataagggta acattcgata ctggaacagg
     2941 ctatggctgg atgagaaatc gggaagaggc ctcgcaaaac gaggcctctg gagagcgatt
     3001 aatcgcaggc aacgatcttc attgccaggc cgccgcgaga ggtttcgcgg tacttggcgt
     3061 tcatatcttt acctgtttcg tacatggttt cgataacttt atcgaggcag acgcgcggct
     3121 cgctggtacg gcgcagcgcc atacgtgcgg cgttcaccgc ttttactgcc gcaatggcgt
     3181 tacgctcgat gcatggcacc tgtacctgtc cggcgaccgg gtcacacgtc agaccgaggt
     3241 tgtgctccat ggcgatttcc gccgcgatgc acacctgcgc cgggcttgca cctaacagtt
     3301 ctgccagacc agccgccgcc attgagcacg ccacaccaac ttcaccctgg caacccactt
     3361 cggcaccaga aatcgacgcg ttcatcttat aaagagaacc aatggcgctg gctaccagca
     3421 ggtaacgagc cagtgagtta gcgttcactt cgcggataaa cttatcgtag tacgccagca
     3481 ctgccgggat aatcccgcac gcaccgttag tcggcgcagt caccacgcga ccgccagcag
     3541 cgttctcttc gttcactgcc agtgcaaaca tgttgatcca gtcaacaacc gccatcgggt
     3601 cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca cggcgtggaa
     3661 cacgcagttt gccaggtaac acaccttcgg tggaaatacc gcgctcaata ccgccacaca
     3721 tcacttccca gacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc agcgccagct
     3781 cgtttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt ttttgcagat
     3841 cggctgctga actgtacgga taaggaactt caaccggtgc gctatcctgc tggccaaaat
     3901 gctcttcatc aacgataaag ccaccgccaa tagagtagta agtctgactg taaacgactt
     3961 tatcacccgc cagcgcggta atgcgcatac cgttttcatg cagagaaagg ttgtcggcat
     4021 ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt gccagcatca
     4081 ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata tccacggtat
     4141 ccggcaggtt acccgccagg cccataataa tggcgatatc agtgtggtgg cctttaccag
     4201 tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta agcaggttac
     4261 gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg gtatgagaac
     4321 tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga aatacatcgc
     4381 gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg aacagagagt
     4441 agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg ctgatgtgac
     4501 cgctgtactt acgcattgcc ggtactttct gaattgcgta catcggcatc aggaacagga
     4561 tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc gggttcaggg
     4621 tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc agcttgttga
     4681 tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg aagccttcac
     4741 gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg ataatcggag
     4801 ccatccacgc gataaccggt gcgttaaagt ggttagccag gtaagacaga atcgagatgt
     4861 tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag ctgaagacga
     4921 agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatcttc gagcatttct
     4981 gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag atgatcggag
     5041 agtggttgaa cgagaacacc attaccggaa ttgccagcca cagggtcatc cacagaccgt
     5101 ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg ttccactgcg
     5161 ggatcaggta cagagccagc agcatcagta cgccaacaaa cgggaatacc agaatactca
     5221 tcgctttaac gatcatctgt tcaccaaagc gaacgatggt catcataccc acgatcagga
     5281 tcagcgacag aatcgcacgc ggcggtggtg tcatacccag ctggtgagac atgaagcttt
     5341 caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata gcgaagaagt
     5401 agagcagggt aatcagttta cctgcgccaa taccaaagtg ttcttctaca acctcggtaa
     5461 tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg tgagcaaaaa
     5521 acgtcatcgg gaacgcaagg atagccatga tgatcagcgg gatcataccg ccaacaccgg
     5581 cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc
     5641 acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc gtgctggttt
     5701 gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt agccagttca
     5761 gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta tcggcgatgg cttaatcatt
     5821 taccgccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct atcagtgatc
     5881 ttgatcccaa atacaataat ctacttggaa gatagattat cccaacgctt tactctgtaa
     5941 atcaaaagta aaagagaatt taagggcaat atttgcc
//
LOCUS       DBFIFS010000247         5970 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.247, whole genome shotgun sequence.
ACCESSION   DBFIFS010000247 DBFIFS010000000
VERSION     DBFIFS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.247"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            141..659
                     /locus_tag="N7A07_004225"
     CDS             141..659
                     /locus_tag="N7A07_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU2478767.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            869..3010
                     /locus_tag="N7A07_004226"
     CDS             869..3010
                     /locus_tag="N7A07_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2478768.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3086..>5970
                     /locus_tag="N7A07_004227"
     CDS             3086..>5970
                     /locus_tag="N7A07_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU2478769.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1358 a         1596 c         1929 g         1087 t
ORIGIN      
        1 accgtaaatt ctagctcggt ttgtatgtaa aaaatactga aaaatgatgc ggttccgtga
       61 agtgagctgc atctgcctgt ctgggattaa cggcaggtca ttgtaagtag taattaacca
      121 cgttaacaag gagtttacaa atgccaacac cgtgttatat ctccatcact ggtcagaccc
      181 agggaaatat tactgcaggt gcatttaccg ctgattctgt cggcaacatc tatgtgcagg
      241 ggcacgaaga cgagatgctg gtgcaggaat tcctgcataa cgttactgtc ccgaccgatc
      301 ctcagtccgg tcagcctgcg ggtcagcgtg ctcacaagcc tttcattttt accgtggcgc
      361 tgaacaaagc ggttccgctg atgtacaacg cactggcctc cggcgaaatg ctgccaacca
      421 ctgaacttca ctggtggcgt acctctgtgg aaggcaaaca ggagcattat ttcactaccc
      481 gcctgactga ttcgacgatt gtcgatatga aactgcatat gccgcattgc caggacccgg
      541 cgaaacgcga gttcacccag ttgctcgaag tttcactggc ctaccgcaaa attgaatggg
      601 aacacgtcaa atctggcacc tccggtgccg acgactggcg cgcaccgctg gaagcataag
      661 ttaagccaac agcatccggc tatcaggccg gatgctgttt ttgtattacg ccgtagaaag
      721 aaaggttgtc agtaaggggt tacgttcacg ggagatggcg gctgccggaa acttcggcgg
      781 tagcgtgcct gaacgcaagc gtagcactgt actgtcacag ccgctttaac agcgaactgt
      841 ctgtttttcc ttaaccccgg aggttgctat gtcaaccgga ttacgtttca cgctggaagt
      901 ggacggcctg ccaccggatg cttttgcggt ggtctccttt catctgaacc agtcactctc
      961 ttcgcttttt tccctcgctc tctctctggt cagccagcag tttctctccc ttgaattcca
     1021 gcagatcctc gacaaaatgg cctacctgac gatatggcag ggcgatgacg tacagcgccg
     1081 ggtgaaaggt gtggtgacct ggtttgaact gggggagaac gacaaaaacc agaagctgta
     1141 cagcatgaag gtatgcccgc cgctgtggcg cacagggctg cgccagaact tccgtatctt
     1201 ccagaatgag gacatcgcaa gcatcctcgg cacgatattg caggaaaacg gggtgaccga
     1261 gtggagcccg ctgttcagcg agccacatcc ttcccgtgag ttttgtgtcc agtacggtga
     1321 gactgattac gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga
     1381 gcacgcgcaa aaaagtaccg accagagcct ggtcctgtgc gataccgtgc tttatctgcc
     1441 ggagtccttt gagatcccct ggaatccgaa cacccgtacc gaggtaagca ccctctgcat
     1501 cagccagttt cgctacagcg cacaaatccg cccttcttcc gtggtgacca aagactacac
     1561 ctttaaacgc cccggctggg ccggacgttt tgaccaggaa ggccagcacc aggattacca
     1621 gcgcacacag tatgaagtgt atgactatcc cggacgtttc aagggcgccc acgggcagaa
     1681 ctttgcccgc tggcagatgg acggctggcg aaacaacgca gaagtggcgc gcggaacaag
     1741 ccgttcgccg gagatatggc cgggacggcg aattgtgctg acggggcatc cgcaggcgaa
     1801 cctgaaccgg gaatggcagg tggtggcaag tgaactgcac ggtgagcagc cgcaggcggt
     1861 gccgggacgc aggggttcag gcaccacgct ggataaccat tttgcggtaa taccggcaga
     1921 cagaacatgg cgaccacagc cgttgctgaa accgctggtg gacggcccgc agagcgccgt
     1981 cgtgacggga ccggcaggcg aggaaatctt ctgtgatgaa catggccgcg tgcgggtgaa
     2041 atttaactgg gaccgctata acccgtcaaa ccaggacagt tcatgctgga tccgtgtggc
     2101 acaggcgtgg gcaggcaccg ggtttggtaa cctggcgata ccgcgtgtgg gtcaggaggt
     2161 cattgtggac ttcctcaacg gcgatccgga ccagccgatc attatggggc gtacctacca
     2221 ccaggaaaac cgcacacccg gtagcctgcc ggggacgaag acgcagatga ccattcgttc
     2281 gaaaacctat aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga
     2341 acaggtctac atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac
     2401 ggatgtgata aacaaccatg ccgaaaaaat aggtaacaac caggcgatca ccgttaccaa
     2461 taaccagatc ctgaacattg gcgttaatca gatacagacg gttggtgtca accaggtgga
     2521 aacggtaggc agtaaccaga ttatcaaagt gggatcaaac caggttgaaa aggtggggat
     2581 cattcgtgcg ctgacggtgg gtgtggcgta tcagacgacg gtaggcggca ttatgaatac
     2641 ctcggtggcg ttgttgcagt cctcacaggt agggctgcat aaatcactga tggtgggaat
     2701 gggctacagc gtcaatgtag ggaataacgt caccttctcg gtgggcaaga cgatgaagga
     2761 aaacaccgga caaacagcag tttattctgc cggtgagcat cttgaactct gctgtggtaa
     2821 ggcaaggctg gtgctgacga aggacggaag catatttctt aatggtacgc acattcatct
     2881 ggaaggggag tcggatgtga acggtgatgc gccggtgatt aactggaact gtggtgccac
     2941 acaacctgta ccggatgcgc ctgtgccgaa agatttaccc cccggaatgc cggatatgcg
     3001 gcaattttga gtcaggtgca aaaaatcaaa acggaactgg tgcagtgacc agataaacca
     3061 tattaaaagc agaaggaatt gattcatggg aggaaaaccg gcggcgcggc agggtgacat
     3121 gacccgcaag ggactggata ttgtgcaggg ttcagcaggg gtgctgatag gtgcgccgac
     3181 gggcgtggcc tgctcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
     3241 gctgggtgcg aaggtgctgc cgggcgagac ggaccttgcg ctgcccggcc cgctgccgtt
     3301 tattctttcc cgcgcctaca gcagctaccg gaccagaaca cccgcgccgg tgggggtgtt
     3361 tggtcccggc tggaaagcgc ccttcgatat ccgcttacag atacgcgatg aaggcctgat
     3421 actcaacgac aacggcggca ggagcattca ctttgagccg ctgtttcccg gcgagataag
     3481 ctacagccgc agcgagtcgt tctggctggc gcggggcggg gtggcggagc agcacagttc
     3541 gcagccgcta agcgcgctct ggcaggtgct gccggaagat gttcgcctga gtccgcatat
     3601 gtacctggcg acaaacagcc ttcaggggcc gtggtggata ctcaactggc cggagcgggt
     3661 gccgggggcg gacgaggtgc tgccgccgga gccgcccgca taccgggtgc tgacgggcgt
     3721 ggtggatggc ttcgggcgga cgctggcctt tcaccgggcg gcggagggtg atgtggcggg
     3781 cgcggtgacg ggggtgacgg acggcgcggg ccgccgtttt cacctggtgc tgaccacaca
     3841 ggcacagcgg gcggaagtat tccgtaaaca gcgcgccacg tctttatctt cccctgccgg
     3901 tccccgttct gcttcctctt ctctggtttt ccccgacacg ctgcccgccg gtacagaata
     3961 cggtgccgat aacggtatcc ggctggaggc ggtatggctg acacacgacc cggcataccc
     4021 ggatgaactg cccgccgcgc cgctggcgcg ctacacgtac acggccagcg gagaactgcg
     4081 ggcggtgtat gaccgcagcg ggacgcaggt gcgcgggttt gcttatgatg cggagcacgc
     4141 cgggcggatg gtggcgcacc attatgcggg taggccggag agccgctacc ggtatgatga
     4201 taccggccgg gtgacggagc tggtcaaccc ggaggggctg gactaccgct ttgagtacgg
     4261 gcaggaccgt gtgaccatca cggacagcct gaaccggcgg gaggtgctgt acacggaagg
     4321 cgagggtggc ctgaaacgtg ttgtgaagaa ggaacatgcg gacgggagca tcacccgcag
     4381 cgagtatgat gaggcgggga ggctgaaggc acagacggat gcggcgggac ggcggacgga
     4441 gtacagcctg catatggcgt cgggtgcggt gacagcggtg acggggccgg acggcaggac
     4501 ggtgcggtat ggctataaca gccagcggca ggtgacgtca gtgacgtacc cggacgggct
     4561 gcgcagcagc cgggagtatg atgagaaggg aaggctggcg gcggagacct cgcgcagcgg
     4621 agagacgacg cggtacagct atgatgaccc ggcgagtgag ctgccgacag ggatacagga
     4681 cgcgacgggc agtacaaaac agatggcatg gagccgttac ggtcagctgc tgacctttac
     4741 ggactgctcg gggtacacga cgcggtatga gtatgaccgg tacggtcaac aaatcgccgt
     4801 tcaccgggaa gaaggcatca gcacttacag cagttataac ccgcgtggcc aactggtcag
     4861 tcagaaggat gcgcaggggc gtgaaacccg ttatgagtac agcgccgcag gcgacctcac
     4921 cgctatcgtt gccccggacg gcagccgcag tgagatacag tatgatgcgt ggggaaaggc
     4981 cgtcagcacc acgcagggcg gtctgacgcg cagcatgggg tatgacgctg ccgggcgcat
     5041 caccgtgctg accaacgaga acggcagcca gtccacgttc cggtatgacc cggtggacag
     5101 gctgactgaa cagcgcggtt ttgacggccg gacgcaacgt taccactatg acctgaccgg
     5161 aaaactcacg cagagtgaag acgaggggct tgtcaccctc tggcactacg atgcgtcgga
     5221 ccgcatcacg caccggacgg tgaacggcga cccggcagag cagtggcagt atgatgagca
     5281 cgggtggcta accaccctca gccataccag tgaaggccac cgggtgtcgg tccactacgg
     5341 ctatgacgat aaaggccgcc tgacgggtga acggcagacg gtggagaacc cggagacggg
     5401 ggagatgctg tgggagcatg agacggggca cgcgtacagc gaacaggggc tggcgacccg
     5461 tcaggagccg gacggtctgc cgccggtaga gtggctgacg tatggcagcg gttatcttgc
     5521 ggggatgaag ctgggcggaa cgccactggt cgagtacatg cgggaccggc tgcaccgtga
     5581 gacggcccgc agcttcggcg gggaggcata tgaacttgcc accgcctgga ataccagcgg
     5641 ccagctccgg agcaggcacc tgaaccttcc gcagcttgac cgtgactacg actggaacga
     5701 caacggacag ctaatccgca tcagcggccc gcaggagagc cgggagtacc gttacagtga
     5761 cacgggaagg ctgacgggcg tccacaccac ggcagcgaac ctggatatcg atatcccgta
     5821 tgcaacggac ccggcaggaa accggctgcc ggacccggaa ctgcatccgg acagcacgct
     5881 cacggcgtgg ccggataacc gcatcgcgga agatgcgcac tatatctatc gctacgatga
     5941 atacggcagg ctggcggaga agacggaccg
//
LOCUS       DBFIFS010000248         5926 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.248, whole genome shotgun sequence.
ACCESSION   DBFIFS010000248 DBFIFS010000000
VERSION     DBFIFS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.248"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            91..1204
                     /gene="ompN"
                     /locus_tag="N7A07_004228"
                     /pseudo
     CDS             91..1204
                     /gene="ompN"
                     /locus_tag="N7A07_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1345..1779
                     /gene="uspF"
                     /locus_tag="N7A07_004229"
     CDS             1345..1779
                     /gene="uspF"
                     /locus_tag="N7A07_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU2478770.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1955..2890
                     /gene="ttcA"
                     /locus_tag="N7A07_004230"
     CDS             1955..2890
                     /gene="ttcA"
                     /locus_tag="N7A07_004230"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU2478771.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3019..4392)
                     /gene="dbpA"
                     /locus_tag="N7A07_004231"
     CDS             complement(3019..4392)
                     /gene="dbpA"
                     /locus_tag="N7A07_004231"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU2478772.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4425..4595)
                     /gene="ynaL"
                     /locus_tag="N7A07_004232"
     CDS             complement(4425..4595)
                     /gene="ynaL"
                     /locus_tag="N7A07_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU2478773.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4870..5853)
                     /gene="zntB"
                     /locus_tag="N7A07_004233"
     CDS             complement(4870..5853)
                     /gene="zntB"
                     /locus_tag="N7A07_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU2478774.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5867..5914)
                     /gene="ydaN"
                     /locus_tag="N7A07_004234"
     CDS             complement(5867..5914)
                     /gene="ydaN"
                     /locus_tag="N7A07_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU2478775.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1561 a         1499 c         1404 g         1462 t
ORIGIN      
        1 tttatcggct aactgaactt ctcctttagg atgttttcac tccccgaaat ggggataaaa
       61 ggcaaataaa ataactaaag gatttattca atgaaaagca aagtactggc acttttaatt
      121 cctgccctgc tcggcgcagg tgctgcacat gcagccgaag tttataataa agacggcaac
      181 aaattagatc tgtatggcaa agttgatggc ctgcattatt tttctgataa ttcagcgaaa
      241 gatggcgacc agagctatgc gcgtctgggt tttaaaggcg aaacccaaat taacgatcaa
      301 cttactggct acggtcaatg ggaatacaat attcaggcaa acaacactga atcttcaaaa
      361 aaccagtcat ggacccgtct ggcatttgcc ggactgaaat tttcagatta cggttctttc
      421 gattacggac gtaattatgg gctggaccga tatgctgcct gaatttggcg gtgactctta
      481 taccaatgca gacaacttta tgactggtcg tgccaatggc gtcgcgactt atcgtaatac
      541 tgatttcttc ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag gtaacaacga
      601 aggtgccagt aatggtcagg aaggcaccaa caacggacgt gatgttcgcc atgaaaacgg
      661 tgacggctgg ggtctttcca caacatatga tttaggcatg ggctttagcg ctggtgcggc
      721 atacacctct tctgaccgca ccaatgacca ggttaaccat actgcggcgg gtggtgataa
      781 agcagacgcg tggactgctg ggctaaaata cgatgctaac aatatttacc tggcaaccat
      841 gtattcagaa acgcgtaata tgaccccgtt tggcgacagc gattatgctg ttgcaaacaa
      901 aacccagaac tttgaagtca ctgcacagta ccagtttgat tttggtctgc gtccggcagt
      961 ctctttcctg atgtctaaag gccgtgacct gcacgctgcc ggtggtgcag acaacccggc
     1021 aggtgttgat gataaagatc tggttaaata cgccgatgtt ggcgcgactt actatttcaa
     1081 taaaaacatg tccacttatg ttgactataa aatcaacctg ttggatgaag atgacagttt
     1141 ctacgctgcc aacggcatct ctaccgatga tattgtcgct ttaggtctgg tttatcagtt
     1201 ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc ttattcttcc tcctttgatc
     1261 taaattaaaa atgtgaactc cgtcattaca caaaaagtgt catctggcgt tacactttat
     1321 gcggatacta aaacaggagg ttttatgaac agaacgattc ttgtccctat cgatatttcc
     1381 gattcagaat taactcaacg cgtgattagc cacgttgagg cagaggcaaa gattgatgat
     1441 gcagaggttc atttcctgac ggtaatacct tcactgccct actatgcctc tctgggttta
     1501 gcgtattccg cagaattacc ggcaatggat gacctgaaag cggaagccaa atcgcaactg
     1561 gaagagatca ttaaaaaatt taaactgcca accgacagag tgcatgtcca tgttgaggaa
     1621 ggctcgccca aagaccgcat tctggaattg gcgaagaaga tccccgctca tatgatcatc
     1681 attgcttccc atcgaccgga tatcaccact tatctgctcg gttccaacgc cgcagctgta
     1741 gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac actaacgccc gcacattgct
     1801 gcgggctttt tgattcattt cgcaaatgtg ctgacatttt cccctctaat ccgtaccata
     1861 cacgccacag tttttatatc agatttctta tgctgggcgt tccggcatga cgcatactct
     1921 tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag aaattagcaa
     1981 gaaagaacaa tacaacctga acaaattaca aaaacgcctg cgtcgtaacg tgggcgaagc
     2041 cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc tctccggggg
     2101 taaagacagc tataccatgc tggagattct gcgcaatttg cagcaaagcg cgccaatcaa
     2161 tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg aacacgttct
     2221 gcccgagtat cttgaaaagc tgggcgttga gtacaagatt gttgaagaga acacttacgg
     2281 tatcgtgaaa gagaagattc cagagggcaa aaccacttgc tcactgtgtt ctcggctacg
     2341 tcgcggtatc ctttatcgca ccgcaacgga actgggggcg acgaagatcg cgttgggcca
     2401 ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg ttctacggcg gcaagatgaa
     2461 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgttattc gtccgctggc
     2521 ctactgccgc gagaaagata ttcagcgatt tgccgatgca aaagcgttcc cgattattcc
     2581 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtgattgctg acatgttgcg
     2641 tgactgggat aaacgttatc cagggcgtat cgagacgatg ttcagcgcga tgcagaatgt
     2701 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatca cccacggttc
     2761 tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa gagatcccac tacaaccggc
     2821 gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
     2881 agtgaaataa ctcggatagc gcccgatgcg caaacgtatc gggctactct aatggaggct
     2941 gaataagacg cggtcagcgt cgcatccgac aatcccgacg aagatgttta cttttgcatc
     3001 aggcaattca acattttatt attttaataa ccgcacccgg cacgtttttc ctttaatctt
     3061 cccgccctgt aactgtttcc atgctttatg agcaacagcc tgacggaccg cgacatagac
     3121 atgcgccgga tgcacggcga ttttgccaat atctgcgcca tcaagcccga tatctcctgt
     3181 cagtgcacct aatacatcac ccgggcgcat tttggctttt ttcccgccat cgatacacaa
     3241 cgttgccatt tctgcttcca gcgtcacaat ggaactatta gctggtggcg tttgccagtt
     3301 aagttttatc tgcaacatgt cagaaatgat attggcccgc tgtgcttctt ccggagcaca
     3361 gaaactgatc gccagaccgc tatttcctgc acgagctgta cgaccgatgc gatgtacatg
     3421 aacttcaggg tcccacgcca gctcaaagtt caccaccagc tcaagcgatt taatatccag
     3481 accacgcgca gcaacatcag tcgcgaccag tacacgggcg ctaccgttag caaaacgtac
     3541 cagggtctga tcacgatcgc gttgctccag atcgccgtgt aatgacaatg cactttgccc
     3601 tacttcattc agcgcgtcgc agacagcctg gcaatctttt ttggtattgc aaaacaccac
     3661 gcaagaggat ggctgatgca agcttaataa ccgttgcaac agaggaattt tgcctttgct
     3721 ggatgtctca taaaattgtt gttcaatggg tggcaaagca tctgttgagt caatttcaat
     3781 cgccaaagga tcgcgttgca ctcgtccgct gattgcggcg atggcttccg gccaggttgc
     3841 cgaaaacaga agcgtctgtc gagatgcagg cgcaaaacgg atgacatcat caatggcatc
     3901 gctaaatccc atatccagca tgcggtcggc ctcatccatc accaaagtat tcagcgcatc
     3961 cagtgatacc gtgccttttt gcaggtgatc cagcaaacgg cccggcgttg ccacgataat
     4021 atgcggcgca tgttgcaacg aatcacgctg cataccgaac ggttgaccac cgcacaacgt
     4081 caaaattttg gtatttggca gaaaacgcgc cagccgacgc aattcacctg ccacctgatc
     4141 cgccagttca cgcgtaggac acagcactaa agcctgggtt tgaaatagcg acgcatcaat
     4201 ttgctgtaac aagccgaggc caaaagccgc cgttttgccg ctgccagttt tcgcctgcac
     4261 gcgaacatct tttccggcaa ggatcgccgg aagcgcggcg gcctgcaccg gcgtcatggt
     4321 taaataaccc aactcattaa ggttcgtgag ttgggcggga ggcaaaacat tcagggtaga
     4381 aaaagcggtc acaatctatt ctcgtggtca tcgacgcaaa gtcatcaggc gcgtatcctc
     4441 gcagatctac gctcacgatg cgacaattta atcggttctt catcgggtgg tgggtcaggc
     4501 atgggttgcg ggcgagggat cggatcgggc actggaacag gatcgccagg aatcggttca
     4561 gggacaggaa tttgcaaata aataagtgtc gtcatatttc cctccggtca ttgggtggac
     4621 tcttaaaggg tagacgctga taaataacag gcaaaaaaaa gccgactcat caaagtcggc
     4681 gtcgtacgaa tcaattgtgc tatgcagtaa ttcaaaaaag gaagtaagac aatatggagc
     4741 gcaacgccca tcgcttgacg ttgcattcac ctgcaagaga gatattgccc tgaatgggta
     4801 gagagtttat tgacttcgct caaactttgc ggcgtttttg tatacagaca gccggaaaaa
     4861 ttgcttttgt tacaaccatt tactacgatg caaccataaa gcaacaccac caataagaac
     4921 aactaacaga atacaaaaaa ttgaaaatcc gaattgccac ccgccgccag ggatcccacc
     4981 aaggttgacg ccaaataacc ctgtcagaaa ggtactgggt aaaaagacca ttgccatcaa
     5041 tgacattgta taggtacgac gagctaaatt ttcctgcatc acctgagcga tttcatccgc
     5101 catcacacca gtccgtgcta tacaggcgtc gatttcgtca aggccgcgcc caaggcgatc
     5161 ggcaatatcc tgcatccgac ggcgttggtc atcgctcatc cacggcaaac gttcactggc
     5221 aagacgagca taaacatcac gttgcggtgc catatagcga cgcatcacaa ttaattgttt
     5281 gcgcagcaga gccaggaatc cacgcggcgg aatttgctga tcaaggagat tatcttcaag
     5341 gtcgataatt ttatcgtgca gctgctcgat aaattcgctg gaatgatcgg tcaacgcatc
     5401 gcacacatcc accagccatc ccccgcaatc ggtcggaccc gtgccctctt ccagatcact
     5461 caccacatcg tccagcgcca gcactttgcg ttgtcgggtc gaaacaatta accgcccgtc
     5521 catatataca cgcatggcga ccagttgatc ggggcgttca tcggtgctgc cgtttataca
     5581 gcgcaatgta atcagcgtgc cttcaccgag acggctgact cggggccgtg tgctctcgcc
     5641 cgccagcgca tcacgtacgt tattgggaag cagcggtgtt gtcgccagcc attgggcgct
     5701 atcatggtgt acataattaa ggtggagcca gcagggatgc gcttcatcaa tcacatctgt
     5761 attttccagc ggtttaacgc cgcctctacc atccagcatc caggcaaata ctgcatccgg
     5821 gacattaacg tccgatccct taatcgcttc cacagtgcct ccatcatcaa cgcattattt
     5881 tgtagtctag ccttctggcc ctgttacgca acatctcatc acccca
//
LOCUS       DBFIFS010000249         5912 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.249, whole genome shotgun sequence.
ACCESSION   DBFIFS010000249 DBFIFS010000000
VERSION     DBFIFS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.249"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..866
                     /locus_tag="N7A07_004235"
     CDS             <1..866
                     /locus_tag="N7A07_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478776.1"
                     /translation="NCDSKHSNGAYHEIYYTADPAPGMVYSTTASGLAFYYLNEYVDV
                     GTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLKTGADAKISFRIKRSI
                     NGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSCQINAGQVIYFDFDTI
                     PASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTGTNRSSDDTMVATDNA
                     DVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAAPASFTGARPQPGVFN
                     ATATLTIEFVN"
     gene            1078..2118
                     /gene="pstS"
                     /locus_tag="N7A07_004236"
     CDS             1078..2118
                     /gene="pstS"
                     /locus_tag="N7A07_004236"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU2478777.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2205..3164
                     /gene="pstC"
                     /locus_tag="N7A07_004237"
     CDS             2205..3164
                     /gene="pstC"
                     /locus_tag="N7A07_004237"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU2478778.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3164..4054
                     /gene="pstA"
                     /locus_tag="N7A07_004238"
     CDS             3164..4054
                     /gene="pstA"
                     /locus_tag="N7A07_004238"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU2478779.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4237..5010
                     /gene="pstB"
                     /locus_tag="N7A07_004239"
     CDS             4237..5010
                     /gene="pstB"
                     /locus_tag="N7A07_004239"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU2478780.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5025..5750
                     /gene="phoU"
                     /locus_tag="N7A07_004240"
     CDS             5025..5750
                     /gene="phoU"
                     /locus_tag="N7A07_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU2478781.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         1415 a         1468 c         1562 g         1467 t
ORIGIN      
        1 gtaactgtga ttcaaaacat agcaatggcg cttatcacga aatatattat accgcagacc
       61 ctgctcccgg tatggtttat agcaccaccg caagtggtct tgctttttac tatcttaacg
      121 aatatgtcga tgtgggaaca aaaatatctg tgctaaatgc ggggtatacg gcagttcctt
      181 ttgaacatgt ttccaaccag gcaactacaa cagatcacac ttgtcaggga aacaaaacta
      241 cagcggttgg cgtgagcctg aaaactggag cagatgcgaa gatttcattt cgtattaaac
      301 gttcaataaa tggaacggta gtaataccta tcaccgatat tgcattgctg tatgccaaca
      361 tatccagcac cacgacccgt ggtgaggcga ttgcaaaagt tcgaatttca ggcagtttga
      421 ccgcaccaca gtcttgtcag ataaatgcag gacaggtgat ttattttgat tttgatacta
      481 ttcctgcgtc cgaattttca tctaccgccg ggcaagccat tacttcacga aaaatcacta
      541 aaacagtgag tattgagtgt acggggatgg ggtatgagcg tacgcagaaa gtcgatgctt
      601 cttttacggg gacgaaccga agcagtgacg atacgatggt ggcgacagac aatgctgatg
      661 tcgggatcaa aatttacaat aaatcgaatg ctgaagttag cgtcaacaac ggcaagttac
      721 ccgcagacat gggcaacacg accatttttg gtcgtaaaaa tggttcggta actttttcgg
      781 cagcacctgc cagctttacc ggtgcccggc ctcagcccgg cgtttttaac gctaccgcga
      841 ccttaaccat tgaatttgta aactaacctt ttgcggctgt tttatgacag ccgttcatat
      901 ttcctctcct ttcatgaaaa aatgctgata tttttcccct tcaatatcaa taagtaataa
      961 ctttcctttg ggtgtcatca aactgtcaca ttccttacat ataactgtca cctgtttgtc
     1021 ctattttgct tctcgtagcc aacaaacaat gctttatgaa tcctcccagg agacattatg
     1081 aaagttatgc gtaccaccgt cgcaactgtt gtcgccgcga ccttatcgat gagtgctttc
     1141 tctgtgtttg cagaagcaag cctgacaggt gcaggtgcaa ccttccctgc gccggtgtat
     1201 gccaaatggg ctgacactta ccagaaagaa accggtaata aagttaatta ccagggtatc
     1261 ggttcttccg gtggcgtaaa acagattatc gccaataccg ttgattttgg tgcttctgac
     1321 gcgccgctgt ctgacgaaaa actggctcag gaaggtctgt tccagttccc gaccgtgatt
     1381 ggcggcgtgg tgctggcggt taacattcca ggtctgaagt ctggtgagct ggtgctggat
     1441 ggtaaaaccc tcggcgatat ctacctgggc aaaatcaaga agtgggatga tgaagccatc
     1501 gccaaattga atccgggtct gaaactgcct tcacagaaca ttgctgtagt gcgccgcgca
     1561 gatggctccg ggacttcctt cgtcttcacc agctacctgg cgaaagtgaa tgaagagtgg
     1621 aaaaacaacg ttggtactgg ctctaccgta aaatggccga tcggtctggg tggtaaaggt
     1681 aacgacggta tcgctgcgtt cgttcagcgt ctgccgggtg caattggtta cgttgaatat
     1741 gcttatgcga agcaaaacaa cctggcgtac accaaactga tctccgctga tggtaaacct
     1801 gttttgccga ccgaagaaaa cttcgctaat gcagcaaaag gtgcagactg gagcaaaacc
     1861 ttcgctcagg atctgaccaa ccagaaaggc gaagatgcgt ggcctattac ctctaccacg
     1921 ttcattctga tccacaaaga tcagaagaaa ccagaacaag gcacagaagt gctgaaattc
     1981 ttcgactggg cgtacaaaac cggggctaaa caggcgaacg acctggatta cgccagcctg
     2041 ccggatagcg tagttgaaca ggttcgcgct gcgtggaaga ccaatattaa agacagtagc
     2101 ggcaagccgc tgtactaata aaactccagg ccggataagg cattaaacgg cttatccggc
     2161 attacaaaat gactttgtaa acgcgtttaa ctgaagagta acttatggct gcaaccaagc
     2221 ctgcttttaa cccaccgggt aaaaagggcg acataatttt cagcgtgctg gtaaaactgg
     2281 cggcgctgat tgtgctattg atgttgggtg gcattattgt ctctctgatc atctcctcat
     2341 ggccgagcat tcagaaattt ggtctggctt tcctgtggac caaagagtgg gatgcgccga
     2401 acgatatcta cggggcgctg gtgccgatct acggtacgtt ggtgacttcg tttatcgcgc
     2461 tgctgatcgc cgtcccggtg agtttcggta tcgccctgtt cctgactgag cttgcgcctg
     2521 gctggctgaa acgcccgctg ggtatcgcca ttgagctgct ggcagccatt ccaagtatcg
     2581 tttacggcat gtggggcctg tttatctttg cgccgctgtt cgccgtttac ttccaggagc
     2641 cggtcggcaa tatcatgtcg aatatcccga ttgtcggcgc gctgttctct ggcccggcat
     2701 ttggtatcgg tatcctcgcg gcaggcgtga tcctcgccat catgattatt ccgtacattg
     2761 cggcggtaat gcgcgatgtg ttcgaacaaa ccccggtgat gatgaaagag tcggcctacg
     2821 gtattggctg caccacctgg gaggttatct ggcgtatcgt tcttccgttc accaaaaatg
     2881 gtgttatcgg cggcattatg ctggggctgg gccgcgcgct tggtgaaacc atggcggtga
     2941 cctttattat cggtaacacc taccagctcg acagcgcctc gctgtacatg ccgggcaaca
     3001 gtatcacctc tgcgctggcg aacgaatttg ccgaagcgga atccggtctg cacgttgccg
     3061 cactgatgga actgggcctg atcctgtttg tgattacctt catcgtcctc gccgcatcga
     3121 agtttatgat tatgcgcctg gctaagaatg agggggctcg ctaatggcta tggttgaaat
     3181 gcaaaccact gcggcgctgg cggaatcccg ccgcaaaatg caggcgcgtc gccgcctcaa
     3241 aaaccgtatt gcactgacgc tctcaatggc gacgatggcc ttcggtctgt tctggctgat
     3301 ctggatttta atgtccacca tcacccgcgg tatcgacggt atgtcgctgg cgctgttcac
     3361 tgaaatgacg ccgccgccca atacggaagg tggtggtctg gcgaacgctc tggcaggtag
     3421 cgggctgtta attttgtggg ccacggtatt cggtacgccg ctgggcatta tggcggggat
     3481 ttatctggct gaatatggtc gtaaatcctg gctggcagaa gtgattcgct tcattaacga
     3541 cattctgctc tctgcgccgt cgattgtggt tggtctgttt gtttacacca tcgtggtggc
     3601 gcagatggag cacttctccg gttgggcggg tgtgattgcg ctggcgttgt tgcaggtgcc
     3661 gattgttatc cgcaccaccg agaacatgct gaaactggtg ccggacagcc tgcgtgaagc
     3721 ggcttatgcg ctgggtacac cgaagtggaa gatgatctcc gcgattacgc tgaaagcgtc
     3781 ggtgtccggg atcatgaccg gtatcctgct ggcgattgcc cgtattgctg gtgaaaccgc
     3841 gccgctgctg tttaccgcgc tctccaacca gttctggagc acggacatga tgcagccgat
     3901 cgccaacctg ccggtgacga tctttaagtt tgcgatgagc ccgtttgcgg aatggcagca
     3961 actggcctgg gccggggtat tgatcattac cctgtgcgta ctgctgctga acattctggc
     4021 gcgcgttgtt ttcgcgaaga ataaacacgg ttgatattgc tgacacggtt ttcccctcac
     4081 cctaaccctc tccccagagg ggcgagggga ccgaccgagc gcctttttga ctctgtacac
     4141 ggttaacact ttgccggatg cggcgtgaac gcctgatccg gcctacggta agcctgattc
     4201 gcgaagtgca tcaggcacga tgaggaaaag attgcaatga gtatggttga aactgccccg
     4261 agtaaaattc aggttcgtaa tttgaacttc tactacggca aattccatgc cctgaaaaac
     4321 atcaacctgg atatcgccaa aaaccaggta acggcgttta tcgggccgtc cggctgcggt
     4381 aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac tgtacccgga acagcgtgcg
     4441 gaaggtgaaa ttctgctcga tggcgacaac atcctgacca actctcagga tatcgccctg
     4501 ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc cgttcccgat gtccatctac
     4561 gacaacatcg cctttggcgt tcgtctgttt gagaagctct ctcgtgccga catggacgag
     4621 cgcgtgcagt gggcattgac caaagccgca ttgtggaacg aaaccaaaga taaattgcat
     4681 cagagcggtt actctctctc tggtggtcag caacagcgtc tgtgcattgc gcgtggtatc
     4741 gccattcgcc cggaagtgct gctgctcgac gaaccatgtt cggcgctcga ccccatctcc
     4801 accgggcgta ttgaagagct gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc
     4861 acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc
     4921 gaattgattg agttcagcaa cacggacgat ctgttcacca agccagcgaa gaaacaaaca
     4981 gaagactaca tcaccggtcg ttacggttga ttcaggagtg cgttatggac agtctcaatc
     5041 ttaataaaca tatttccggc cagttcaacg ccgaactgga aagtatccgc acgcaggtga
     5101 tgaccatggg cggcatggtg gagcagcagc tttctgatgc aatcaccgcg atgcataacc
     5161 aggacagcga tctggcgaag cgcgtgatcg aaggcgacaa gaacgtcaac atgatggaag
     5221 tggcgatcga tgaagcctgc gtgcgcatta ttgccaaacg ccagccgacg gcgagcgacc
     5281 tgcgattggt tatggtgatc agtaaaacca ttgccgaact ggagcgtatt ggcgacgtgg
     5341 cggacaaaat ctgccgtact gcgctggaga aattctccca gcagcatcag ccgttgctgg
     5401 taagtttgga gtcgctgggc cgtcatacta tccagatgct gcacgacgtg ctggacgcgt
     5461 tcgcgcggat ggacattgac gaagcggtac gtatttatcg tgaagataaa aaagtcgatc
     5521 aggagtacga aggtattgtt cgtcaactga tgacctacat gatggaagat tcgcgcacca
     5581 ttccgagtgt acttaccgcg ctgttctgtg cgcgttctat cgaacgtatt ggcgaccgct
     5641 gccagaatat ttgtgagttt atcttctact acgtgaaggg tcaggatttc cgtcatgtag
     5701 gcggtgatga gctggataaa ctgctggcgg gaaaagaccc caaagagtag tgctaactgt
     5761 aggccagata aagctttttg ccgcatctgg caatcagccc ggtcatacat agttaccggg
     5821 ctgatacact ttctcactga aatattgcca cacaaactca cctcgaaact ctccccagta
     5881 cattgaatat acattatttc atttgaatta tt
//
LOCUS       DBFIFS010000250         5880 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.250, whole genome shotgun sequence.
ACCESSION   DBFIFS010000250 DBFIFS010000000
VERSION     DBFIFS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.250"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            110..556
                     /locus_tag="N7A07_004241"
     CDS             110..556
                     /locus_tag="N7A07_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU2478782.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            567..2006
                     /locus_tag="N7A07_004242"
     CDS             567..2006
                     /locus_tag="N7A07_004242"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU2478783.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1996..3066
                     /locus_tag="N7A07_004243"
     CDS             1996..3066
                     /locus_tag="N7A07_004243"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2478784.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3066..4814
                     /locus_tag="N7A07_004244"
     CDS             3066..4814
                     /locus_tag="N7A07_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU2478785.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4864..>5880)
                     /locus_tag="N7A07_004245"
     CDS             complement(4864..>5880)
                     /locus_tag="N7A07_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU2478786.1"
                     /translation="IMALGLTGCDDKKAETETPLPANSQPAAPAPEAKPTEAPVAKAD
                     AKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQGPTGKES
                     TVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYY
                     TQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREG
                     KTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFI
                     NSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDY
                     NSLR"
BASE COUNT         1367 a         1500 c         1486 g         1527 t
ORIGIN      
        1 atccttcact ctgctttata ttgtcacgac ttcacgacat atccgcgctc atcacaaacc
       61 cgccccgcta ttacaattaa ctgaaatatt ttttcatgga gtccgattaa tggcagctct
      121 tactgcaagc tgtattgacc tgaatattca gggcaatggc gcttattccg ttctgaagca
      181 gttggcgaca atggcgttac aaaacggttt tatcaccgac tcacaccagt tcctgcaaac
      241 cctgctgctg cgcgaaaaaa tgcactccac cggttttggt tctggtgtcg ccgtgccgca
      301 cggtaaaagc acctgcgtta aacaaccgtt cgtgttattc gcccgcaaag cgcaggctat
      361 tgactggaaa gccagcgacg gcgaagacgt caattgctgg atctgcctcg gcgtaccgca
      421 aagtggcgaa gaggatcagg tcaaaatcat cggcacactg tgtcgcaaaa ttattcacca
      481 ggaatttatt catcaactgc aacagggcga taccgaccag gtgcttgcct tgttaaatca
      541 aaccctcagc tcataaggaa gtggcgatgg agtcatcctt acgtattgtc gcgatcacca
      601 actgccccgc cgggatcgct cacacctaca tggtggcgga agccctggaa cagaaagccc
      661 gttctctcgg ccataccatt aaagtggaaa ctcaagggtc cagcggcgtt gaaaaccgct
      721 tatccagcga agagattgcc gctgccgatt acgtgattct cgccaccggg cgtggcctga
      781 gcggtgacga tcgcgcacgg tttgccggga aaaaagttta tgagattgcc atctcccagg
      841 cgctgaaaaa tatcgatcag attttcagtg aattaccgac aaattcgcag ctttttgccg
      901 cagatagcgg cgtgaagcta ggcaaacagg aagtacaaag cggcagcgtg atgagccacc
      961 tgatggcggg cgtctctgcg gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc
     1021 ttgccaacat gctggtgcag ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt
     1081 cgtttacctg ggtagttgaa tccatcggtt accttggctt cacctttatg atccccatca
     1141 tgggcgctta tattgcctcg tcgattgccg ataaaccggc tttcgctccg gcgtttctgg
     1201 tttgctatct ggcgaacgac aaagcgctgc tcggcaccca gtcgggtgcg ggattcctcg
     1261 gcgcggtggt gctggggctg gcgattggct atttcgtctt ctggttccgt aaagtccgtc
     1321 ttggcaaagc attacaaccg ctgctcggct ccatgcttat cccattcgtt actctgctgg
     1381 tttttggcgt gctcacttac tacgttatcg ggccggtgat gtccgacctc atgggcgggc
     1441 tactgcactt cctgaatacc attcctccat caatgaagtt tgcggcagcg tttctggtcg
     1501 gtgcgatgct ggcgttcgat atgggcggcc ccatcaataa aaccgcctgg ttcttctgct
     1561 tctcactgct ggaaaaacac atttatgact ggtacgccat cgtcggcgtt gtccccgtcg
     1621 cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa aaagaggctg
     1681 ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctatt ccttacgctc
     1741 tggcagctcc gttgccgatg attaccgcca atacgctggc gggcggcatt accggcgtgc
     1801 tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt gacccgctga
     1861 tcggcctgat gtcgccggta ggttcgtttt atctggtgct ggcgattggc ctcgcgctga
     1921 acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg aaagccgcgc
     1981 aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc cagcgccgtc
     2041 agcggcgatg aacaggaagt tcgcgacatt ctgataaaca cgctggaacc ttgcgttaat
     2101 gagatcacct ttgatggtct gggcagcttt gttgcccgta agggaaataa aggtccaaaa
     2161 gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat cgacgagagc
     2221 ggttttctgc gctttaccac cattggcggc tggtggaatc agtcgatgct caaccaccgg
     2281 gtaacaatac gcacacacaa gggagtgaaa atccctggtg tgattggttc cgtagcgcct
     2341 catgcgttaa cggaaaagca aaagcaacaa ccgctgtcat ttgatgagat gttcattgat
     2401 attggcgcga acagtcgcga agaagcgaaa aaacgcggtg cggaaattgg cgattttatt
     2461 agcccggaag ccaattttgc ctgctggggc gaagataaaa tagtcggcaa ggcgctggat
     2521 aatcgcatcg gctgcgcgat gatggctgag ctactacaga cagtaaataa cccaggaatt
     2581 acgctgtacg gcgtcggcag cgtggaagaa gaagttgggc tacgcggggc acaaacctcg
     2641 gctgaacaca ctaaaccgga tgtggtgatc gtgctggata ccgccgtcgc gggcgatgtt
     2701 ccgggcattg ataacattaa atacccgctg aaactgggca acgggccggg gctgatgctg
     2761 tttgacaagc gctacttacc caaccagaaa ctggtggcgg cgttaaaaaa ctgtgccgca
     2821 cataacggtt taccgctgca attttccacc atgaaaaccg gagcgacgga tggcgggcgc
     2881 tacaacgtaa tgggcggagg gcgtccggtt gtcgcgctgt gtctgccaac tcgttatctg
     2941 cacgctaaca gcggtatgat ttcaaaagcc gattatgatg ctctgctcac gctgatacgg
     3001 gattttctga cgaccttaac tgcggagaaa gtcaacgcgt ttagccagtt ccgtcaggtg
     3061 gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa cagccgcgca
     3121 cgcctggcga gctggcgcaa cagactggcg tttcaggcag gaccatcctg cgtgatattg
     3181 actatctcaa ctttaccctt aacggcaaag ctcgtatctc tgccagtggc agtgcgggct
     3241 atcagttgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag cacgataacg
     3301 acgatcggct gctggcgctg ttattactga atacctttac tccccgtgcg caactcgcct
     3361 cggcgcttaa tttgccagaa acttgggtcg cagagcgtct gccccggtta aaacagcgtt
     3421 atgaacgcac ttgttgcctg gccagtcgtc ccggtttggg ccatttcatt gatgaaacag
     3481 aagagaaacg cgttatcttg ctggcgaact tgctacgcaa agatccgttt ttaatacctt
     3541 tggctggcgt aacacgagac aaccttcagc atttatccac ggcctgcgac aaccaacacc
     3601 gctggccgct catgcagggg gattatctct ccagcctgat tctggcgatt tacgccctgc
     3661 gtaatcaact gaccgatgag tggccgcaat atcccggtga cgagataaaa caaatcgttg
     3721 aacataacgg tctgtttctt ggtgataacg ctgtaagaac cctgacaggt ttgatagaga
     3781 aacagcatca gcaagcgcag gtaatttctg ccgatcatgt gctggggttg ctgcaaaggg
     3841 tgccaggtct cgcgtcattg aatattattg atacgcagct ggttgagaac attaccggtc
     3901 atttattacg ttgccttgcc gcaccagtgt ggattgctga gcatcgccaa agcagcatga
     3961 ataacctgaa agccgcctgg cctgcggcct ttgatatgag tctgcacttt attacgctac
     4021 tgcgtgaaca gctcgatatt ccccttttcg acagcgatct gatcggtttg tattttgcct
     4081 gtgcgctgga acgacatcaa aacgaacgcc agccgatcat tttgctctcg gaccagaacg
     4141 cgattgccac tattaatcag ctcgccattg agcgcgatgt tttacattgt cgggtgatta
     4201 ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga gattgagccg ttattgatca
     4261 ttaacaacag ccattattta ctggatgacg cggtaaataa ttacatcacc gtaaaaaata
     4321 tcattacggc tgccggtatc gaacaaataa aacattttct ggcgacggca tttattcgcc
     4381 agcaaccgga gcgttttttc tctgcccccg gaagttttca ttattcgaat gtacgcggtg
     4441 aaagctggca acatattacc cggcaaattt gtgcgcaatt agtcgcacaa caccatatta
     4501 ccgccgatga agcgcaacgg atcatcgccc gcgaaggcga aggtgaaaac ctgattgtta
     4561 atcgcctcgc catcccacat tgctggagcg aacaggagcg acgttttcgt ggatttttta
     4621 ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat taaccatgtc ttgatcgcct
     4681 gcgccgccgc cgatgcgcgt catgagctaa aaatatttag ctatctggca agcgtattgt
     4741 gtcagcatcc ggcagaagtt attgccgggc taacaggata tgaggcattt atggagttac
     4801 ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac actgcattta tcatcaaaaa
     4861 cgtttaacgc aggctattat agtcatcaac catttcgttg tactcgcgta acgctcgtgg
     4921 aaatgagtct tcgaccatcc agcttgtgtt tgcatcctga agatgctctt tatcccagtc
     4981 agagtacggg actttatctc tgacgcggcg aacatatttt ttagtctcga gttgatactg
     5041 gtctgccgaa ttaatgaacg agaaattcat gccgccttta tccgcttctt tggcctgcgc
     5101 caccagcgtt tccagttcag acactttctt cattgccgca tcaacatcaa acttctcttg
     5161 cgatatcagg ttattaattt gtttcgctga aatcatgact gccagagaat agtagtggaa
     5221 tgttttccct tctctttctt caatattttt cagttccgca agctggcgct tatcattaat
     5281 ttcctgaatc gccgcatgat aagattctgc tacaggttca aaggcttcca gattttttaa
     5341 gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg tctttataat tttcctgggt
     5401 gtaatattta tccatttcgt taatggtatt acccaacgcc acggcagcat cgatataact
     5461 caccgccacg ccatcaattg gttgcagcgc aggcgttaac gccacagcac tttttacgcc
     5521 tttttcacac tcagcgaggt tggattcact aatgccgtag atgccataaa cagtactctc
     5581 tttaccggta ggcccctgtt taaaatcttt cagccagtca gcataacgcg ccagactgcg
     5641 ctgtaccggg atctgtaact tgttgtagca cttgatatag acatccattt tttcgtcgaa
     5701 aaccgcttgt tcatcgacca gcggttgcgc aggtgtttca ggtttagcat ctgcttttgc
     5761 aacgggagct tcagtaggtt tcgcttcagg agctggtgcg gcaggttgac tattggcagg
     5821 caggggcgtt tctgtttcgg cttttttatc atcacaaccc gtcagaccga gggccatgat
//
LOCUS       DBFIFS010000251         5796 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.251, whole genome shotgun sequence.
ACCESSION   DBFIFS010000251 DBFIFS010000000
VERSION     DBFIFS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.251"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            231..791
                     /locus_tag="N7A07_004246"
                     /pseudo
     CDS             231..791
                     /locus_tag="N7A07_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            999..1355
                     /locus_tag="N7A07_004247"
     CDS             999..1355
                     /locus_tag="N7A07_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478787.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(1643..2569)
                     /locus_tag="N7A07_004248"
     CDS             complement(1643..2569)
                     /locus_tag="N7A07_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2478788.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            2726..3646
                     /locus_tag="N7A07_004249"
     CDS             2726..3646
                     /locus_tag="N7A07_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2478789.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            3881..5023
                     /locus_tag="N7A07_004250"
     CDS             3881..5023
                     /locus_tag="N7A07_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HGU2478790.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            5497..5597
                     /gene="ykgR"
                     /locus_tag="N7A07_004251"
                     /pseudo
     CDS             5497..5597
                     /gene="ykgR"
                     /locus_tag="N7A07_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT         1756 a         1281 c         1179 g         1580 t
ORIGIN      
        1 gaaatacgtc gaagtaggta tatacgccag gataaaccga tatatttttt atggatattc
       61 attttccttc atcggactgt aaattgcgga gatatcgaac ttgcactacc catcgcaaca
      121 ctcaaacaga tttatgaatt tcgttcataa cccctgacaa atcctgccgt ctaatcgagc
      181 tcttctcttt acgttatgct tctcatgaac gataacggaa ggataacacc atgcaaaaag
      241 taaaactgaa taacggtatt gaaatgcccc tgctgggctt tggtgtattt cagatgaccg
      301 atgccgccga atgcgaaaga gcggtcattg acgctattaa caccggttat cgactgattg
      361 acaccgccgc ctcctatcaa aatgaacccc aggtcgggaa cgcactgaaa caaagcggta
      421 tcgcccgtaa cgaactcttt gtcaccacga aactctggtt gcaggatacc agttacgaag
      481 gggccaaagc ccagttcgaa cgctctctga atcgactgca acttgattac gttgacctgt
      541 atttgattca tcagccttac ggtgatgtcc acggtgcctg gcgtgccatg gaagagctgc
      601 aacaggccgg taaaattcgc gctattggcg tcagtaactt ccatcctgac cgactggctg
      661 accttatcgc ctttaataac gtcgttccag cggtaaacca ggttgaagtt aaccctttca
      721 atcagcagct acacgccgtt ccgtggatgc aaagccgtgg tattcagcca gaagcctggg
      781 caccgtttgc agttacaggg cggtaagcga ttatgcatct acctatttac gtcggtcact
      841 cagtcagagt cttgcccata ttcaatgagg ttcaacaccg cagccaatga aaagtaggcc
      901 atgcggggat taatagcatt actcattaac caatcgctct tttcatttac gtgcttttta
      961 tgtaacatat tgcttttatt gatcttcctg gagtttgcat gaataagaat ctcatcctcg
     1021 catttgcatt attttcctta cctgtatttg cagaagaaga tctggggcca ggcaaatatg
     1081 tttgtgacat taggatttcc agcctggata ccgctactca aattctgtca aaatccgcaa
     1141 cagttctcga taatggaaac aatttcatcg ttcaaatgcc aaatggagat caattgtact
     1201 ctccagatct ggaaaatgtt gatgatggta taaaacaaaa agcaacaata ggtggagtga
     1261 catttattcg tcgtccaact tttaacgatc gcttcatcgt ggaagatgga aatacaggat
     1321 tcttctataa gatgcgaaac tgcgagaaga aataaaggaa gtttgatagc gatatcctcc
     1381 tgatttcagg ctttgtacct gttcaagact tacaactaca agccgctacg tgttaacgcc
     1441 tcccaaaata accaccgtta accgtagcgg tcatagatac tattatttta accacattac
     1501 tggtgataat gataattgat attatggccg tatagaaaat aagtattaat tatcagaaac
     1561 tttattatgt aaacgcatcc taatgattac gttattcctc ttaccgatat tcgcccagag
     1621 caaccatcaa ttacacatat catcaatttt cgagttttac tcttttctcc accataaaat
     1681 caataaaagc acgaacttta gagggcattc tcacccgtga tgaaacataa atataaaacc
     1741 caggaaatga aacagcccaa tcctcaagaa tcggctgtaa cttgcccgca ttgatatgtt
     1801 cagcgattgc aaaatccaga tgttgaatga tacccgttcc attcagcgcc attcgtatca
     1861 tgctttcgtc atcattagaa atatacttac ctgaaacctc tatttcgaac tcttcattac
     1921 cccccacaac cttagaaaac tcccatgcag agagtgcacc gctactttta tgccgcaacc
     1981 gaaggcaatt atggttactc agttcctgag gcgtttcagg aataccgtgt tcttttaaat
     2041 aatctggcga tgccacagta accagtttaa taagcggaga aacaggaacg gcagtcatat
     2101 gctcatccag tccttgctca agacgtatgc ccacatcaca gccgtccgca ataatgttag
     2161 ataaaccgtc atccataatg agttctatct ttgttttggg gtaacgagta agaaactcgc
     2221 caatatgagg ctcaacaagc atccgtgctg caacacggga ggaatttatt ctcaacaggc
     2281 ctgtaggttc aagttgggta tcaccaacat ctttcaaagc atcatcaatt tgccccaaag
     2341 ccgacaccag cacttcatac agacgctgtc cttcctcagt aagcgagata ttgcgggtcg
     2401 tacggtatat cagcttggtg ttaagacgtt cctcaagaga ttttaaattc tgggacaatg
     2461 cggccagcgt aatacccatt tcagaagcgg ctctggaaaa actgccggca cgaacaatca
     2521 gcgcaaacca ggcaagagaa ggcaaaattg aaggctctat tttcatcatt gtaaacctta
     2581 acttaacact gtgatagata ttagaccatt gatgataacc aatacaagtc atactattac
     2641 gccgttctcg atatgaacac aaatgccagt tgcggataat agaaatgata tttaaatctg
     2701 tttaggcaaa tccccggagc ttgaaatgaa aactgtttcg ataaaacaca cttactggga
     2761 tatagctgct gatatctatt tccctgacga ttttaatagt gaaaaaaagt atcctgccat
     2821 tatcagcgcc catccaattg gaagctgcaa agaacagaca tcgggtagtg tgtatggtgc
     2881 cgccctggca aaagctggct ttatcgtcat tgcttttgat gctagttttc agggaagcag
     2941 cggtggggaa ccgcgttatc tggaagatcc aacaatgcga gtaaaagact tcagcattgt
     3001 cgttgattac cttaccagct taccttacgt tgatgccggg cgtattggtg tactgggtat
     3061 ttgtggtgga ggtggatatg ccattaatgc cgcaatgaca gaacgccgaa ttaaagctat
     3121 tggtactgtc acgggtgcaa actacgggcg tcttatgcgt gagggattta cagcgtttaa
     3181 tcctatcgga gcacttgaag caatggcgca acaaagaaca gacgaagcta acggcgcaaa
     3241 acttcgcgta gatgatttac tcccctcttc tccggatgca gctcttgaag cgggactaac
     3301 agaaatagac ctttttgaag ccacagaata ttatcgttcg ccacgaggct atgcacctaa
     3361 tggggtaaat cgttcgctat tctcacatca gactgttgcc gtgggttggg atgcatttca
     3421 tcttgcagaa gtgctgttaa ctcaaccaat aatggttgtg gtgggcgata gagtgggcgc
     3481 atttggtgcc taccgtgacg gctgcgaaat tattggccgc gctgcttcaa aacataaaga
     3541 gctggtcgtg gttgaaggtt attcacatta cgacctgtat gataagccag aacctgtgaa
     3601 acaggcactc gataaattaa ttccttttta taaaacacat ttgtaataat tttacaggga
     3661 gatcccttat ctccctgaca tttatccttt attgactaca caacaataat aaaattattc
     3721 aaaaagaaat ttcattaaaa aacaaaaaag cagcaactca attattagaa accctttaaa
     3781 gggattataa ataaagccag aactcttggt tccaaagctg gcaacaacta accagttaaa
     3841 acatacgcat caaaatcaga taaacatttg aggtattgtt atgacaaata aacacccatc
     3901 attgttttct ccttttatgc tgacagaaaa aataaagcta cgtaatcgta tcgttatggc
     3961 tcctatgacg acctggtcag cgaatcctga tggaaccatc tctgagcaag aactcgaatt
     4021 ttataaacgt cgctcacaaa atgttggttt agtaataaca ggatgtacct atgtcacgcc
     4081 aagcggcatt gggtttactc atgaatttgc cgcgtatgac gaccgtttca ttaacagcct
     4141 tgaaaagtta gcagcggcag ccaaaagtgg cggtgctccg gctattttgc agattttcca
     4201 cgccggtaat aaagccatcc cggagcttgt accaaataac gatgttatta gtgccagcgc
     4261 atcaagcgta aaatctggtg actttatgaa gagagtggta caaagcaggg aaatgactga
     4321 aaacgaaatt caggaaacca ttagagcatt cggagatgtc acaaaaaggg cgattaaagc
     4381 aggttttgat ggcattgaac ttcatggtgc gcacggattc cttctacaga attttttctc
     4441 ccctctattt aaccagcgta atgaccgatg gggaggagat ctcgaaggac gtatgcgctt
     4501 cccgctggcc gtattacagg aagtaaaaaa tgtagtctat gaatatgcga caaagccctt
     4561 tgccatcggt tacagaatat ctcctgaaga atctgcaaca ggggggctgc ggatagagga
     4621 tacttataaa ctccttgatc ggctcatttc atccggtatt tcgtatatac atacctcgct
     4681 ggtcagtatt aacgatagct accctgttga gtcgccaaat ggcccccgaa ctattgaatt
     4741 gattttaaat catattgcag gcagagtgcc ggtaatcgct gcaggtaaaa tacgcacacc
     4801 gtctcaggct caagaagcaa tttccactgg cctgccactt gttgcgatcg gtaaagggct
     4861 ggtgataaat ccagaatggg tgactttagc tgaatcaggt agaggccatg aaattcaaac
     4921 aacccttaat ccacaacttg ttcctgaatt aaccattccg gataaactat gggatcaaat
     4981 tcaggcatcg aaaggaactg gctggtttcc gttaatggat taaatatcta ttactctccc
     5041 gagagtaaaa ctatcaaatc aatgagtaac cgatacagcc tcacaaattc tttcagataa
     5101 ttaacccgtc actgcggcac gttctctcat aaggcgtgcc tgcagtgtaa tctagtatgt
     5161 atccggcata gttgacggtt taatagagct caattcctcg cccaggttac caacaacgat
     5221 atcttgtata acaccccagg agatacgaca ttatgatgag atgcgatatg ctcctcagcg
     5281 ccaatcaacg ccaggactgg gccagtcttg agatcagact cattcccgga actgacttca
     5341 ggcgacatat atcaatgacg ttggggcaat aaaagatgcc tgatgtaata catctattca
     5401 aatttgtgat acatgtcaaa aaattggttg accaaactcg ttatttatat aagggcactt
     5461 acgaagtgcg ctctttttta aagcgaggaa gtaccaatga aagagaataa agtacaacaa
     5521 atcagtcata aactgattaa tatcgttgtt ttgtcgcaat tgtagaatac gcctatttat
     5581 ttctccattt ctattaataa cggcaataaa ctgttcactt cagtgatatt taaaatatgc
     5641 atcctctccc tttttgtaag taattattat atccgtggga gaggaataca cgctgtcagg
     5701 taatcaatca tactgcgata aatcatcggc cagtaaagtg gagataacct ccattctcga
     5761 aaaatccata ctctcagcga aactatcatc aatcac
//
LOCUS       DBFIFS010000252         5782 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.252, whole genome shotgun sequence.
ACCESSION   DBFIFS010000252 DBFIFS010000000
VERSION     DBFIFS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.252"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            277..2709
                     /gene="hsdR"
                     /locus_tag="N7A07_004252"
     CDS             277..2709
                     /gene="hsdR"
                     /locus_tag="N7A07_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGU2478791.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2776..4245
                     /locus_tag="N7A07_004253"
     CDS             2776..4245
                     /locus_tag="N7A07_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU2478792.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4245..>5782
                     /locus_tag="N7A07_004254"
     CDS             4245..>5782
                     /locus_tag="N7A07_004254"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU2478793.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFS
                     RQYFA"
BASE COUNT         1732 a         1355 c         1364 g         1331 t
ORIGIN      
        1 tcatataccc aatcaaacaa tcaacaataa cattatcttt cttgctcgaa agaaacgcaa
       61 ctataagatt tttatatatt ttgttagagt tgatatggtt cctgtaactt ctgtttatct
      121 gtctctgata tgcccagatt aattcaagca agatataaca gagatatcaa tgtgatctct
      181 gctacgtact ttacgttgaa acaccgccct aaatcatagc caggctattg attttatgta
      241 caatcagaat cgtcctgata aaagcgagaa taccccatgg cggaactgaa cctaagtaac
      301 ctgacggaag cagacatcat taccaaatgc gttatgccag ccattctcaa tgcaggctgg
      361 gacaacacaa cgcaaatcag acaggaggtc aaactccggg acggtaaagt catcgtacgt
      421 ggtaaagttg cggcacgcag aacggtaaaa tctgctgaca tcgtgctgta tcacaaacct
      481 ggcattccct tagccgtgat tgaagcaaaa gccaacaaac atgaaattgg caaagggatg
      541 caacagggca ttgaatatgc gcgcctgctg gacgttcctt ttgtttttgc caccaacggt
      601 gatggcttta tcttccgcga tgccaccgca gccgaaggtg aatgccttga aaagcaaata
      661 acgctggatg acttcccctc ccccgctgaa ctctggcaaa aattctgcct ctggaaagga
      721 tacacacaag ctcagcttcc ggtgattact caggactatt acgacgatgg cagcggtaaa
      781 tcgccacgtt attaccagct tcaggcaatc aacaaaacca ttgaagccgt ctccaacggg
      841 caaaaccgcg ttctgctggt catggcgacc ggaacaggga aaacctatac cgcattccag
      901 atcatctggc gcctgtggaa atcaaaaaat aaaaaacgca ttttgttcct tgccgatcgc
      961 aatattctgg tcgaccaaac caaaaataat gatttccagc catttggtac ggcaatgacc
     1021 aaagtcagcg gacgcaccat tgatcccgct tatgaaattc acctcgcgct ctatcaggct
     1081 ataactggcc cggaggaaga ccaaaaagcg tttaaacaag tcgcaccaga tttcttcgat
     1141 ctgatcgtga tcgacgaatg ccatcgcggc agcgcatccg aagacagcgc ctggcgagaa
     1201 atccttgatt attttagttc cgccacccaa attggcttaa ccgccacgcc aaaagagacg
     1261 catgaagtct ccagcacgga ttacttcggc gatccggttt acgtctactc actaaaagaa
     1321 gggatcgaag acggcttcct cgccccttat aaagttgtcc gtgttgatat tgatgttgat
     1381 ctgcaaggct ggcgcccaac caaagggcaa actgacttaa acggcgaagt gatcgacgat
     1441 cgtatctata accagaaaga tttcgatcgc acgatggtaa tcgacgaacg cacagaactg
     1501 gttgccagaa ccattaccga ctacctcaag cgtaccaatc cgatggataa aaccatcgtc
     1561 ttctgtaacg acatcgatca tgcagaacgt atgcgccgcg ccctggttaa tctcaacccg
     1621 gagcaggtga aaaagaacga caaatacgtc atgaaaatca ccggcgatga tgaaattggc
     1681 aaagctcagt tggataactt catcaacccg aaaaaaccgt acccggttat cgcgaccact
     1741 tcagagctga tgaccaccgg tgtggatgct aaaacctgca aactggtagt actggaccag
     1801 aacatccagt cgatgaccaa attcaagcag attatcggtc gtggtacacg catcgacgaa
     1861 cgttacggca aactctggtt taccatcctc gactttaaaa aagccaccga actgtttgcc
     1921 gatgagcgtt tcgatggcat tcccgaaaaa gtcatggata ccacaccaga ggatatcgcc
     1981 gatccagaat ctgattttga agagaaactc gaagaaatca gcgaacatga cgacgaacag
     2041 gtaacaggcg ttgatgaacc gcctgcgcca ccataccagg ttaaagatac cgatgatgtc
     2101 ggcccacttc cggaagaaga cgagaagaaa atccgcaagt ttcacgtcaa cggtgtagca
     2161 gtgggcgtta ttgcccagcg tgttcagtat tacgacgccg acggtaaact ggttaccgaa
     2221 tcctttaaag attacacccg caaaacactg ctcaaagaat atgcctcgct ggatgacttt
     2281 acccgcaagt ggcaggacgc cgatcgcaaa gaagcgatca ttcacgagct ggagcaacag
     2341 gggatcatct gggaagtact ggcagaagaa gtcggtaaag atctcgaccc gttcgacatg
     2401 ctttgccacg tagtgtatgg tcagccgccg ttaacccgca aagagcgcgc cgagaacgtg
     2461 cgcaagcgga actacttcac aaaatactct gaagcagcgc aagccgtgct cgataatctg
     2521 ctggataaat acgccgatgc gggcgtacag gagatcgaaa gtattcaggt gctgaaactt
     2581 aagccattcg acagcatggg caccttaccg gagattatta aaaccggatt tggcgaccgt
     2641 aacgggtata atcaggcgct cagcgagctg gaaaacgaaa tctaccaatt accgccccgc
     2701 tctgcttaac aactctcttt tgcgccttct gcaaccatca tgttgcagaa ggccttgttt
     2761 ttcatggaat acaacatgtc tatcagctca gtaatcaaat cattacaaga tattatgcgc
     2821 aaagatgccg gtgtggatgg cgatgcgcag cgtctcggtc agctctcctg gctgctgttt
     2881 ttgaaaatct tcgatgccca ggaagaggcg ctggaactgg agcaggataa ctatcaatat
     2941 ccgatcccac agcgttattt atggcgcagt tgggccgcca acgcgcaggg cattaccggt
     3001 gattccctgc tggaattcgt taatgatgat ctgttcccgg cgttgaaaaa cctcactgcg
     3061 cctatcgata aaaacccacg cggctacgtg gtgaagcagg cgttcagcga tgcctataac
     3121 tatatgaaaa acggtacgct actgcgtcag gtgatcaata agctgaacga aattgacttt
     3181 accagcgcca gcgaacgcca tctgtttggt gatatttacg aacagatcct taaagatctg
     3241 caatctgcgg gcaatgcggg cgaattctat actccacgcg ccgtcactcg ctttatggtg
     3301 gatcgcgttg atccgaaact cggcgaatcc attatggacc cggcctgcgg tacgggcggt
     3361 tttcttgcct gcgcatttga tcatgtaaag aacaaatacg tgaagagcgt cgccgatcat
     3421 cagacgctgc aacaacagat ccacggtgtt gagaaaaaac agcttccgca cctgctggcg
     3481 accaccaata tgctgctaca cggcattgaa gtgccagtgc aaattcgtca cgacaacact
     3541 ctgaacaaac cgctttcctc ccgggatgag caactggatg tcattgttac caacccgccg
     3601 tttggtggca cggaagaaga cggtattgag aagaactttc cggcagagat gcaaacccgc
     3661 gaaacggcgg atttgttcct gcaactgatt gtggaagtac tggcgaaaaa tggtcgcgcg
     3721 gcggtggtat tgccggatgg cacactattt ggcgaaggcg ttaaaaccaa aatcaaaaag
     3781 ctacttaccg aagagtgcaa cctgcatacc atcgtgcgct taccgaatgg tgtgtttaac
     3841 ccctataccg gcattaaaac caacctgttg ttctttacca aaggtcagcc aaccaaagag
     3901 atttggttct atgagcatcc gtatccggcg ggcgtaaaaa actacagcaa aactaagccg
     3961 atgaagtttg aagagtttca ggcggagatc gactggtggg gtaacgaggc cgatggtttt
     4021 gccagccgcg tagagaatga gcaggcgtgg aaagtcagca ttgatgacgt gattgcgcgt
     4081 aacttcaatc tggatattaa aaacccacat caggcggaaa ccgtcagcca tgatccggcc
     4141 gagctgttag cgcagtatgc aaaacagcag gcggagatcc agacgctgcg taatcaactg
     4201 cgcgatattc ttggcgctgc gctgtctggc aaggaggtta actaatgagt gtggaaaagc
     4261 tgatcgttga tcatatggaa acctggacct cggcgttgca aacccgttcc accgccgggc
     4321 gcggcagttc cggaaaaatt gatttatatg gcattaagaa attacgtgag ctgattctgg
     4381 aactggctgt gcgcggtaaa ctggtgccgc aggacccgaa cgatgaaccg gcgtcggagc
     4441 tgctgaagcg tattgcggca gaaaaagcag agctggtgaa gcaaggtaaa attaaaaagc
     4501 aaaaaccact gccggaaatt agtgaggaag agaagccgtt tgaattgccg gagggatggg
     4561 agtgggtgcg tattagtgaa attggacatg actggggaca aaaaactcca gataaagatt
     4621 ttacttacat tgatgttggt tcaataaata aagaatatgg aattattgaa gagctatcta
     4681 ttctttcagc gaaagatgcc ccatcgcgag cgagaaaaat tgttcagcaa gggactatca
     4741 tatactctac tgttcgcccg tatttgttaa atatagctat tattgaaaac gaaatactac
     4801 ctgaaccgat cgctagtact gccttcgcta ttattcatcc atatacggcg atggatgcta
     4861 atttcattta ttattatcta cgttctccgg tatttgtttg ttatgttgaa aattgtcaga
     4921 caggggttgc ctatccagca atcaatgaca aacaattttt ttctggaata actccagttc
     4981 ctccatcctt ggaacaagtt cgcatcgcaa acaaaatcaa agaattaatg tccctctgcg
     5041 accaactgga acagcaatcc ctgaccagtc tggacgcaca tcaacaactg gttgaaaccc
     5101 tgttgggaac gcttacagac agccaaaccg ccgaggaact ggctgaaaac tgggcgcgta
     5161 ttagcgagta tttcgacact ctatttacca ccgaagccag cgtggatgcg ttaaaacaga
     5221 ccattctgca actggccgta atgggcaaac ttgtgccgca ggatccgaat gatgaaccag
     5281 cctctgaact gctcaaacga attgcgcagg aaaaagctca actggtgaaa gaaggaaaaa
     5341 taaaaaaaca aaaaccgttg ccgccaatta gcgatgagga aaaaccgttt gaacttccgg
     5401 aagggtggga gtggtgttta tttgaagata ttattgatat tcaaagtggt atcactaaag
     5461 gaagaaattt atcaaataga actttggtaa aagttcctta tttacgtgtt gcaaacgtcc
     5521 aacgcggata tcttgatctt acggaaatta aacagattga aatccctatt gaggaaaaag
     5581 aaaaatatca agtagtcaag ggagatttat tgataacaga aggcggcgac tgggatacag
     5641 tcgggagaac tacagtatgg tgtcatgact ggtatatagc aaatcaaaac catgtattca
     5701 aaggacgaaa tatagggcaa gatgttgatc catattggtt agaaacatat atgaatagcc
     5761 cattctcaag acaatatttt gc
//
LOCUS       DBFIFS010000253         5776 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.253, whole genome shotgun sequence.
ACCESSION   DBFIFS010000253 DBFIFS010000000
VERSION     DBFIFS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.253"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..2021)
                     /gene="pqqU"
                     /locus_tag="N7A07_004255"
     CDS             complement(<1..2021)
                     /gene="pqqU"
                     /locus_tag="N7A07_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU2478794.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIV"
     gene            2263..3324
                     /locus_tag="N7A07_004256"
     CDS             2263..3324
                     /locus_tag="N7A07_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU2478795.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3439..4938)
                     /gene="ansP"
                     /locus_tag="N7A07_004257"
     CDS             complement(3439..4938)
                     /gene="ansP"
                     /locus_tag="N7A07_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU2478796.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5205..>5776
                     /locus_tag="N7A07_004258"
     CDS             5205..>5776
                     /locus_tag="N7A07_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU2478797.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVC"
BASE COUNT         1518 a         1488 c         1400 g         1370 t
ORIGIN      
        1 ttgacaatga cagaaccaac gtattcttta tcgaataaat tatcgacacg accaaacaaa
       61 tccacagtta aattgtggta attgtattta tacccggtga ataagccgac gagagtataa
      121 gacggcgctt tggccgtatt ttcatcatct gccataatat cgcccatata acgcgcttcc
      181 atgcctgcat accaaccatc ttccggtaca taacctatcg acgcaaagcc catattacgg
      241 gcgatccctg gcatccgatt accgttacaa tcctgttcgt tgcaaacatt gctgcgatag
      301 gtcgcatcaa gccaggtcca ggacgcgttt acgcgaaaat ctcccgcgaa acgttgatcc
      361 catgccagtt cagcgccttg acgacgggtc tttccagcat ttttgtaagt cgtacgccca
      421 ccgctactgc tatcgacaac aatttcatca tcggtgtcgg tctggaacaa tgcgagacta
      481 agcagcccat caccaatacg cgttttactg ccgatctcaa ttgtatcgtt ggtggatggt
      541 tttaaaccaa agttcatacc gctttgccca tcagcgcgat aagacagctc attaatcgtc
      601 ggcgtttcaa aaccacgccc ggctgccaga tagatattcc aggcatcggt cattgcatat
      661 tttaacgatc cggctggtag ccatttgtga taactggcat caccgctgtc atcgccgtta
      721 cccggagtaa cgtaatggtc gttggaatca aaccacacgg agctgtagcg cacgccagca
      781 tccagcgaca gtttttcgct cagctgccac tgcgtctgta aatagggatc gatgttccac
      841 atcagattgc gttcgtcgcg acgcaactca cctttttgcc cgtactccgg catgccgcta
      901 ttcaggcgga agttattgta gcccttgcgg ttttcactca tgttttcgta gttcaggccg
      961 gtagtgaacg tgaccggaac gcccagttca ccacggtgtg tccagcggct gtctattccc
     1021 tggtaatggc gttgcagggt aatcacgctg cccgcatgtg acgggttaag ttgtggtgcc
     1081 atgggtattg actggtactg ggtcgtttct cgctctccgg catacatcat cacactcata
     1141 tcatcccgcg aactcaggct acgttcatag cgcaacccag cctgagtttg cttgatggtt
     1201 tttcgcgtgt cgtactgttc tgcacgaggc gcttgttgcg gattcgcttt ccattctgct
     1261 ttggttagcc cacctgggtc atctgctttg atatccacgc tattgaaaat caggcttaat
     1321 ttgctggcgt catcaatgcg tacgcccagt ttggcattag ctaaattttt ctgtgcgcca
     1381 ctatggtcac gatagccgtg ggtcgtaaaa cgcgtggttg agacggtgta atcaacatcg
     1441 ccaggctgtg tgccgtctcc cgttgcgccc gttgctttca gcccatagcg ccagctgcca
     1501 aaactgccgt agtaactact ggcttcaatg gttggtggct gttgcccggt ctgggtggtg
     1561 acattcatta caccgccaga cgcgttgcca tacagggcag agaaggggcc acgcagcact
     1621 tccacatttt gcacactgct taaatcgatg ttggatgttt gcccttgccc gtcgggcatg
     1681 gtagcgggaa taccgtccac atacaggcga ataccgcgaa ttccgtaagt ggagcgggag
     1741 ccaaatccac gaatcgacag ctgtaaatct tgcgcatagt tctgccggtt ttgtacctgc
     1801 aaaccaggca cgccggtcag tgattcggac aagttaatgc gcggtgttgc caggcgcatc
     1861 tcctcgccat ccaccacgct tactgctgca ggggtatcca gttctgaaac cacctgcggc
     1921 gcggcactga caatcatggt ctgttcatca gcggcaaaaa caacggggga aaggacaagc
     1981 agggcgggca aaacggtctg tcgaacgaaa acaatcttca tgaaaaaagc caggttaaga
     2041 atgggaaaac accgtcatgg taatgaaatt gtaaatttat ggaaaacgaa acggcacgat
     2101 acgttaatta attgagagaa ttgtagtcgt aacggcaaga aatgctccac atttgagtaa
     2161 ataatgatta ccattcccat ttataacaag agcgtaacga tgattacgct tagcgaagca
     2221 ttgtgaagca gcaaaaatat cggttcatca aagggagtcg tcatgcattt acgtcatctg
     2281 ttttcattgc gcctgcgtgg ttcattactg ttaggttcat tgcttgttgc ttcatcattc
     2341 agtacgcagg ccgcagaaga aatgctgcgt aaagcggtag gtaaaggtgc ctacgaaatg
     2401 gcttatagcc agcaagaaaa cgcgctgtgg ctcgccactt cgcaaagccg caaactggat
     2461 aaaggcggcg tggtttatcg tcttgatccg gtcactctgg aagtgacgca ggcgatccat
     2521 aacgatctca agccgtttgg tgccaccatc aataacacga ctcagacgtt gtggtttggt
     2581 aacaccgtaa acagcgcggt cacggcgata gatgccaaaa ctggcgaggt gaaaggccgt
     2641 ctggtgctgg atgatcgtaa gcgcacggaa gaggtgcgcc cgctgcaacc gcgtgagctg
     2701 gtagctgatg atgccacgaa caccgtttac atcagtggta ttggtaaaga tagcgtgatt
     2761 tgggtcgttg atggcgagaa tatcaaactg aaaaccgcca tccagaacac cggtaaaatg
     2821 agtaccggtc tggcactgga tagcaaaggc aaacgtcttt acaccactaa cgctgacggc
     2881 gaattgatta ccatcgacac cgccgacaat aaaatcctca gccgtaaaaa gctgctggat
     2941 gacggcaaag agcacttctt tatcaacatc agccttgata ccgccaggca gcgtgcattt
     3001 atcaccgatt ctaaagcggc agaagtgtta gtggtcgata cccgtaatgg caatattctg
     3061 gcgaaggttg cggcaccgga atcactggct gtgctgttta acccagcgcg taatgaagcc
     3121 tacgtaacgc atcgtcaggc aggtaaagtc agtgtgattg acgcgaaaag ctataaagtg
     3181 gtgaaaacgt tcgatacgcc gactcatccg aacagcctgg cgctgtctgc cgatggcaaa
     3241 acgctgtatg tcagtgtgaa acaaaaatcc actaaacagc aggaagctac ccagccagac
     3301 gatgtgattc gtattgcgct gtaatagata tggcgagggt gataacggag agacttgcct
     3361 gactttgatc acgccgcatg cggcacacct tcgtcagtaa tctaaatttg cccggcacgt
     3421 agccgggcaa ttttgcaatt agctcgccgt ttcaggctta aacacaattt cctgtttttc
     3481 ttcatcttcc tcgacgactg gcgcagtgct gtgaatttca gcaacgcgtt tgcgcacacc
     3541 aaaccagcct ataaccagca gaataccgat aatcggcagc gccgcgatag tataagtccc
     3601 gttcgggtaa tcgaacgcca tcagcacaag gacactcagt aaaaacagta atgtcagcca
     3661 ggaagtgaag ggcgcgccag gcagtttaaa actgacatcc gctgctttgc cttctttaat
     3721 cgctttacgc aggcgcatct ggcacacgat gataaacgcc catgaagcga tgattcccag
     3781 cgacgcgaag ttcaacacaa tctcaaatac gcgcgacggc accagatagt tgaggaatac
     3841 gccgacgaca tacacaacca gtgtcgccag aatcccggca tacggcacat gctgacgact
     3901 cattttcgcc ataaaactcg gtgcggaacc gcccatcgcc attgagcgca gaatacgtcc
     3961 ggtgcagtac agacctgaat tcaggctgga gagggcagcg gtcagcacca caatgttcat
     4021 aatgctgccg atatatggca cacccagttt agagaaaaac gtcacgaacg gactttgccc
     4081 cgcctgatac gcgctccacg gcaataacat aaccagcaac accacggagc cgacgtaaaa
     4141 caggccaata cgccaaatca cactgttaat cgctttaggc accatggtct gcggatcttt
     4201 acattctccg gcagctgtac ccaccatttc aatggaggca aaagcaaaca ctacgccctg
     4261 aatcaacacc agcgcaggca gcaaaccgtg ggggaagaag ccgccattat cggtgattaa
     4321 atgaaagcca gtggtgttgc catccagcgg ctgaccacta ccgaggaaca ccgtacccac
     4381 gaccaaaaag gtcacaatgg cgagcacttt aataagcgca aaccagaact ccatctccgc
     4441 aaaccatttc acaccgatca tattcatggt gccaacgatg gtaagtgcag cgagcgcaaa
     4501 gacccactgc ggcacgccgc caaacgcacc ccagtaatgc atatacagag cgacggcggt
     4561 aatatcaaca atccccgtca tcgcccagtt gatgaagtac atccagccag caacataagc
     4621 ggctttctca cccaaaaact cacgggcata agagacaaaa ctgccactgg aagggcggtg
     4681 tagcaccagc tcacccaatg cacgcagaat aaaaaacgaa aacaagccac aaattaaata
     4741 aaccagtgcc agtgctggac ccgccatttg cagtcgggct cctgcaccta aaaacaagcc
     4801 ggtgccaatc gcgccgccaa tggcgatcat ctgtacctgg cgattgccca tcgctttgtg
     4861 atacccctct tcgtgggcat taagccagcg gcgtttcgcg gcgtgttgat ctgaagtgtc
     4921 ggtgtcgtgt ttactcattg ctctccctga ttgctttaat gaaaaagtca tataagttgc
     4981 catgaacaat gtttattcat cctggcgatg atagttatca ccattattct ttatgggtat
     5041 aaaactatcg gcgcagcatc ctacccgtta tcgataaacg atgcaaaaca tccccttaca
     5101 atcctgaagg ggattaatac aactgacgaa aaaatgacaa atccttttgc tggttaacct
     5161 ttgtactgtc ctacatttaa tctttaaaag attgtgaggg gcatatgatt aaggtgtatg
     5221 gcgtacccgg atggggctct acaatcagtg agctgatgct aacactggct gatattcctt
     5281 atcagttcgt tgatgtcagt ggttttgacc acgagggaac ttcacgcgag ttattgaaaa
     5341 ccctgaaccc gttgtgtcag gttccgacac tggcactgga aaatgacgag attatgacgg
     5401 agacagcggc gattgcattg atggttctcg atcgtcgccc ggaccttgcg ccaccggttg
     5461 ggcgtgccga acgtcagcag tttcaacggt tattggtctg gctggttgct aatgtctatc
     5521 caacgttcac tttcgccgat taccctgagc gttgggcatc tgacgcacct gaacagttaa
     5581 agaagaatgt tattgaatat cgtaaatcgc tttatgtctg gctgaattca caattgaccg
     5641 ctgaacctta tgcgtttggt gagcaactaa cgctggtgga ctgttatctt tgcactatgc
     5701 gcacatgggg gcctgggcat gaatggtttc aggataatgc cacgaatatc agtgcgattg
     5761 ctgatgcagt atgtca
//
LOCUS       DBFIFS010000254         5749 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.254, whole genome shotgun sequence.
ACCESSION   DBFIFS010000254 DBFIFS010000000
VERSION     DBFIFS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.254"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..130)
                     /gene="yaiA"
                     /locus_tag="N7A07_004259"
     CDS             complement(<1..130)
                     /gene="yaiA"
                     /locus_tag="N7A07_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU2478798.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISE"
     gene            complement(180..704)
                     /gene="aroL"
                     /locus_tag="N7A07_004260"
     CDS             complement(180..704)
                     /gene="aroL"
                     /locus_tag="N7A07_004260"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU2478799.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(887..1345)
                     /gene="yaiI"
                     /locus_tag="N7A07_004261"
     CDS             complement(887..1345)
                     /gene="yaiI"
                     /locus_tag="N7A07_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU2478800.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1465..2274
                     /gene="proC"
                     /locus_tag="N7A07_004262"
     CDS             1465..2274
                     /gene="proC"
                     /locus_tag="N7A07_004262"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU2478801.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2291..3406)
                     /gene="adrA"
                     /locus_tag="N7A07_004263"
                     /gene_synonym="dgcC"
     CDS             complement(2291..3406)
                     /gene="adrA"
                     /locus_tag="N7A07_004263"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU2478802.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3508..3828)
                     /gene="psiF"
                     /locus_tag="N7A07_004264"
     CDS             complement(3508..3828)
                     /gene="psiF"
                     /locus_tag="N7A07_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU2478803.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3947..5362)
                     /gene="phoA"
                     /locus_tag="N7A07_004265"
     CDS             complement(3947..5362)
                     /gene="phoA"
                     /locus_tag="N7A07_004265"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU2478804.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(5463..5723)
                     /gene="iraP"
                     /locus_tag="N7A07_004266"
     CDS             complement(5463..5723)
                     /gene="iraP"
                     /locus_tag="N7A07_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU2478805.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1346 a         1547 c         1410 g         1446 t
ORIGIN      
        1 gttcactgat caacgagtct ggtttaggat gatcggctct gagttggtac caggttaccg
       61 tctgtcctgg ctttcctttt tcaattgtca ctatatatgc ttcacgagga taaggcggtt
      121 tcgttggcat cgttcttcct tatttcacgg gatgaacgtt aagtataggc gctcgaaaat
      181 caacaattga tcgtctgcgc cagggcgctg cgaatttcag aaatcacctg gctgggttcg
      241 tttgttgcgt cgatgataat atgcgcaact tcgcgatata gcgcatcgcg ttcttccagc
      301 acttcctgaa cttcttcgct cagcggtttt cccgttaagg ttggccgtaa atcttcttcc
      361 ggtgcagctt gcagtcggtt aaccaggact gatactggcg cacacaaata aaccacgatc
      421 ccgttatttt gcatgaagtg acgattaaat tccgtcagaa taatgccgcc gcctgtagcg
      481 ataacggtgg atggcgcagt taccgcttcc agcgccgccg tttctctggc gcgaaatccc
      541 gcccactctt ccctttcgac gatctccgcg accgtcatat tgagctgtga ttgcaaccac
      601 tgatcggtat cgacaaaccg acggttaagc gaatcggcaa gggccattcc gaccgttgtt
      661 ttaccacagc cccgaggccc gatcagaaaa agaggttgtg tcatcgtggg ttttccccaa
      721 taggtcgcaa tgcggcgaaa gccggtgtca tgagaatagc gatcatacca tcaaactagt
      781 acaatttcga ttgtaaagaa aaaattccac ttaaagtgaa aatctcaata catcccttac
      841 tataccaata aatattcaag aatgaagtgt aaataataaa ttacatttag ccacgactac
      901 gttgcacttc cagccaccac ttctccagct ccgcggcaaa ggcctggcgg tcacgttgtg
      961 aaaggctatc tggtccgccg gtctggatcc cactggcacg taaggtatcc ataaaatcgc
     1021 gcatcgtcag gcgctcacga atggtcgctg gcgtgtaacg ttcaccgcgc ggattaagcg
     1081 ccgcagcgcc tttctcgatg gcttcagcag ccaaaggtat atctgcggtg atcaccaaat
     1141 ctcccgcttc acactgccgg acaatttcgt tatcggcaac gtcgaaacct gccgcgacgc
     1201 gcagcgtacg aataaatcgc gatggcggca cgcgtaaact ctggtttgct accagtacca
     1261 gcggcatctg catacgttcc gccgcgcgat acaaaatctc tttaattaca ttgggacacg
     1321 cgtcggcatc cacccaaatt gtcataaagt catcctttgt tgggtaatcc tctattgtgt
     1381 cgcgcttttg ccttccggca tagttctgtt tatgcttctg ccagcgatta tcaaaacaat
     1441 gaatttcacg gcaggagtga ggcaatggaa aagaaaatcg gttttattgg ctgcggcaat
     1501 atgggaaaag ccattctcgg cggtctgatt gccagcggtc aggtgcttcc agggcaaatc
     1561 tgggtatata ccccctcccc ggataaagtc gccgccctgc atgacaagtt cggcatcaac
     1621 gccgcagaat cggcgcaaga agtggcgcaa atcgccgaca tcatttttgc cgccgttaaa
     1681 cctggcatca tgattaaagt gcttagcgaa atcacctcca gcctcaataa agactctctg
     1741 gtcgtttcta ttgctgcagg tgtcacgctc gaccagcttg cccgcgcgct gggccatgac
     1801 cggaaaatta tccgcgccat gccgaacact cccgcgctgg ttaatgccgg gatgacctcc
     1861 gtaacgccaa acgcgctggt aaccccagaa gataccgctg atgtgctgaa tattttccgc
     1921 tgctttggcg aagcggaagt aattgctgag ccgatgatcc acccggtggt cggtgtgagc
     1981 ggttcttcgc cagcctacgt ctttatgttt atcgaagcga tggccgacgc cgccgtgctg
     2041 ggcgggatgc cacgcgccca ggcgtataaa tttgccgctc aggcggtaat gggttccgca
     2101 aaaatggtgc tggaaacggg agaacatccg ggggcactga aagatatggt ctgctcaccg
     2161 ggaggcacca ccattgaagc ggtacgcgta ctggaagaga aaggcttccg tgccgcagtg
     2221 atcgaagcga tgacgaagtg tatggaaaaa tcagaaaaac tcagcaaatc ctgatgactt
     2281 tcgccggacg tcaggccgcc acttcggtgc ggttacgtcc ggctttcttt gctttgtaga
     2341 gcgccaaatc tgccgatttc aaccactcgc gatagtgact catttgtggg ttcagcggcg
     2401 caacccccac actaatccgt aaatttacct gtggcgtatt cggcaaacgt aatgtattta
     2461 gcccttcatg cacccgtaac atggcggtaa tggcgctctc agctggcgta ccggacatga
     2521 ttaccgcaaa ctcatcgccg ccaaaccgac caatcacatc gctaccgcgc agggtaattt
     2581 gtaactgtcg ggtaagcgcc acaatcgctt catcgcccac atcatggccc caggtatcgt
     2641 tgatgctctt gaaatggtcg atatcgataa tcagtaacgt tgcatcgcga ttatgccgcc
     2701 gacagttatc aaattcattg cgtaacatag tttcccaatg acgtcggtta tacacgcccg
     2761 tcatgccgtc gcgggtactc atgacctgca acctgcgttt atgttccgcc agtttggttg
     2821 ccgtctggta gctgacccag ccaaacagca gaggataaat gacaataatg ggaagggaga
     2881 gccaccattc cagcggcgca ctattgaacg acacggtaat gcccgtcagc tcgagggtga
     2941 caaggcagga aaccaccatc aacaccagac ccgcgacaaa cagacggggg ccgcctgccc
     3001 ccatcaaatt cagacacata atcatcaaca tcgcggtgga aggcagcacg tttacgccca
     3061 ttacgcctac ccacattccc gctaatactg catcggtttt taagttgtaa atttcccggc
     3121 taagcggatc gacggccctg ctcgctatct gccaggctaa atgcggccag acgaacgccc
     3181 agccgaccaa caccagccac caccagcccg gcggcgggtg tgaaaccagc gttgaagcaa
     3241 tcggtaagaa catgccagcc aggccaaccg cacggggtag tctgacgcga cgggcgaagc
     3301 gcagcccgga ccgctgatgt tcgttttgag gagttaaagg aggttcctcc ccgtgcgccg
     3361 ccgctttttt gaaaaagttt tcatcattca ttatttttgg gaacattcag aaacaatttt
     3421 cccaaattat agagacgggg ctaaaggcag aagtatgaaa tttcgggtga gccattttag
     3481 tagctcaccc gttagcacta tcaggtatca ggcggctttc ttgaggcagg cgctcataaa
     3541 cttattacga tcatcacctt tcagtgattg ttgcgttgct tgattattac attcgcgcat
     3601 cttttgctgc tgtggcgtca aacttttttc accaggcgca gacttgctgt tcttcaggca
     3661 atcactcatg taggtcttac gagcatcccc tttcaacgcc tgtgccgtcg cctgctgatt
     3721 acaggaggtc atacgctgtt gttgtggggt taaggttctc tcggcagcgc cgacggtggt
     3781 taaaaaaacc agaccgaaaa gcaaggtaac cagtaatgta attttcatag caccatccct
     3841 cttcatgttt taaccatgag cgtatgcgcc cgtgatctga cattaagtct ggttgctaac
     3901 agcaaaaaaa ccacccggca gcgaaaattc actgccggga gcggttttat ttcagcccca
     3961 gagcggcttt catggtgtag aagagatcgg tctggtcggt cagtccaacg acattggcgg
     4021 catgcgggcc atacgccgca atacgcaact gactgccggt atgttcttgt gaatcctctt
     4081 cggagttccc gtaactcatc accatcactg cgccatcttt ggtatttagc gcctgagtga
     4141 ggcccggagc tttggtatcc ggcgcaacaa tctggctggc gtgggcgtga tcagcggtga
     4201 ctatgaccag cgtgttacca tcctttttgg cgaattccag cgcccgttgt acggcttcat
     4261 cgagatcgac cgtctcgcca atttgcccac aaggattcgc agcatgatcc tgtttatcga
     4321 ttgacgcacc ttcaacttgc aggaaaaagc ctttctcatt tttactcaac aattcaatgg
     4381 ctttgtcggt catctgtgcc agggtcggta cactgtcatt acgttgcgga ttaggcgtac
     4441 aggtgactgc gggcttatcg atattaccgt ggtacgttgc tttcggtcct agccagcgca
     4501 ctggcatatt gccgtcagca aacaggccaa gcaggggttt ttgctgattc gcttccgtca
     4561 ccgaattcag tgaggcagca tcgctcacca actgataacc acgcgcctgt gcctgttcac
     4621 gcagcgtttt tccctgccat tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc
     4681 cgccaagcgt aacgtcggca cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc
     4741 ctttttccag agcgttaccc ggacattttt cactggtcgc gctcggaccg tagcatttgc
     4801 gcgaggtcac atgcgccacc agcgcggcgg gcgtggcatc ctgcaactct gcggtagaaa
     4861 cgttaccggt cgccagacct gcggcttttg ccatttccag aatcgttggg tgatcttttt
     4921 cgtgaatatc gacgcccagc gcgccgttat aggttttgac accggttgac caggcggttg
     4981 ctgatgcagc cgagtcggtg acgtagtccg gttttccggt ttttttattc agcgcatagt
     5041 gagtgtattg cccggtaagc ggtaaggcat cgataccttt aaaaaagccg cccgcacctt
     5101 cggcataatt acgtgcggca gtaatttccg aatcccccat cccatcgcca atcagcaaaa
     5161 taatattttt tgcaggttta tcgctaagag aatcacgcag agcggcggtc tgatcagccg
     5221 ttaagcggcg agcaccgccg ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa
     5281 caggcatttc tggtgtccgg gcttttgtca caggggtaaa cagtaacggt aagagtgcca
     5341 gtgcaatagt gctttgtttc actttatttt ctccatgtac aaatacatta aaaaataaaa
     5401 acaaagcgac tataagtctc ggccgtgaca actttatgac agctgttgaa aagattaact
     5461 ttttactgac gaggatgctt caataacttc tttacgtaat cgcgcagcag ctccgtatcg
     5521 tcgtcaggaa tgctggcatc gggctttacc tcgtacagcg ccccctctac ctgatcaatc
     5581 aaccgctgtt ggtcattttg cgccatattg cgaagcattg cagtgacgat aatctccaaa
     5641 gcttctacct gcgcacacag ttctttcgac tcttcttctt tttgggcaag cttaaataac
     5701 aactcagcaa tgagattttt catgtctgta tttccttatc caaagtatg
//
LOCUS       DBFIFS010000255         5628 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.255, whole genome shotgun sequence.
ACCESSION   DBFIFS010000255 DBFIFS010000000
VERSION     DBFIFS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.255"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..936)
                     /gene="ydgH"
                     /locus_tag="N7A07_004267"
     CDS             complement(<1..936)
                     /gene="ydgH"
                     /locus_tag="N7A07_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU2478806.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADL"
     gene            1460..2992
                     /gene="pntA"
                     /locus_tag="N7A07_004268"
     CDS             1460..2992
                     /gene="pntA"
                     /locus_tag="N7A07_004268"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU2478807.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            3003..4391
                     /gene="pntB"
                     /locus_tag="N7A07_004269"
     CDS             3003..4391
                     /gene="pntB"
                     /locus_tag="N7A07_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU2478808.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(4416..5450)
                     /gene="tqsA"
                     /locus_tag="N7A07_004270"
     CDS             complement(4416..5450)
                     /gene="tqsA"
                     /locus_tag="N7A07_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU2478809.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
BASE COUNT         1390 a         1354 c         1484 g         1400 t
ORIGIN      
        1 cagatctgca ctgacggtca ggttattacc acgttcctgc cactggcggg tgatgtggta
       61 atatttagca cctttcttcg cggctcgttt cgcaacctga taggagactt cggtcatgtt
      121 gccgtagttg ccagagaatt tgatgctgtc gaacgggacc atcatcgctg ctgtcgcttt
      181 gttcagttct tcgactttag tgccatccgg aagcgtgaca gtgtaacgcc cgccttttga
      241 tgactgggtt tcaaagaagc gaccgacttc agaacttggt gatgcggtag tcgcaacacc
      301 cgggatctca actttcttcg cggcttcgcc accggcagcc agagctgcac gtcctgcttc
      361 ggaatctgcc gggatcacat ccgggctctg gacgatacgt ttcttagcat cttttttata
      421 gatgaatgca gtaatacgct ggttgccgcc ctggttggca tcgatttgac gaacgatgta
      481 gaaagagtag gcacctttcg cttttgccgc tttggtgatg gcatcattga cttctggctg
      541 gctacgatag aagccctgga cggtgaccgt gtcaaacggt tcaatcagaa cagcctgatc
      601 tttcggcagt tcgacaacac cgttaattac gcgattactt gtttcttctg ctttttcagc
      661 atcggcttta tagaggtcag cgaccacacg ccagttaccg ctgttcccaa aatcagaagt
      721 gtcgacaaca taaaaagagg cggcaccttc tttatctgcg cgacgagaaa cggctttcac
      781 cgcttcgcca atagcattaa aacgaccggt aaccactaca cggtcaaaag gtttaaccgc
      841 tgccgcttgc tccggtgtca gttctgttgc tgcgttaacg gagaatgccg tagcagaaag
      901 cagtgccgac gccaggaggg tgttcttaag cttcataaaa ataatccttc gccttgcgca
      961 aaccaggtac tggtattgtt attaacgaga aacgtggctg attattgcat ttaaacggtg
     1021 taactgtctg cgtcattttt catatcacat tccttaagcc aattttaatc ctgctcaaat
     1081 gaccgtctat gcttaaaaaa cagccgtatc agcatcatta ctactgaagc aactgaattg
     1141 tataagttaa tttaatgtta agtagtgatt cgtgccgggg cgatgtctcg ttttacccga
     1201 ccgtcgaaga caattatcag tctttatccg gcgttctaag gtgtttatcc cactatcacg
     1261 gctgaatcgt taatattttg cgagttcacg ccgaaatact gatttttggc gctagatcac
     1321 aggcataatt ttcagtacgt tatagggcgt ttgttactaa tttattttaa cggagtaaca
     1381 tttagctcgt acatgagcgg cttgtgtggc tcctgacaca ggcaaaccat catcaataaa
     1441 accgatggaa gggaatatca tgcgaattgg cataccaaga gaacggttaa ccaatgaacc
     1501 ccgtgttgca gcaacgccaa aaacagtgga acagctgctg aaactggggt ttaccgtcgc
     1561 ggtagagagc ggcgcgggtc aactggcaag ttttgacgat aaagcgtttg tgcaagcggg
     1621 cgctgaaatt gtagaaggga atagcgtctg gcagtcagag atcattctga aggtcaatgc
     1681 gccgttagat gatgaaattg cgttactgaa tcctgggaca acgctggtga gttttatctg
     1741 gcctgcgcag aatccggaat taatgcaaaa acttgcggaa cgtaacgtga ccgtgatggc
     1801 gatggactct gtgccgcgta tctcacgcgc acaatcgctg gacgcactaa gctcgatggc
     1861 gaatatcgcc ggttatcgcg ccattgttga agcggcacat gaatttgggt gcttctttac
     1921 cgggcaaatt actgcggccg ggaaagtgcc accggcaaaa gtgatggtga ttggtgcggg
     1981 tgttgcaggt ctggccgcca ttggcgcagc aaacagtctc ggcgcgattg tgcgtgcatt
     2041 cgacacccgc ccggaagtga aagaacaagt tcaaagtatg ggcgcggaat tcctcgagct
     2101 ggattttaaa gaggaagcgg gcagcggcga tggctatgcc aaagtgatgt cggacgcgtt
     2161 catcaaagcg gaaatggaac tctttgccgc ccaggcaaaa gaggtcgata tcattgtcac
     2221 caccgcgctt attccaggca aaccagcgcc gaagctaatt acccgtgaaa tggttgactc
     2281 catgaaggcg ggcagtgtga ttgtcgacct ggcagcccaa aacggcggca actgtgaata
     2341 caccgtgccg ggtgaaatct tcactacgga aaatggtgtc aaagtgattg gctataccga
     2401 tcttccgggc cgtctgccga cgcaatcctc acagctttac ggcactaacc tcgttaatct
     2461 gctgaaactg ttgtgcaaag agaaaaacgg taacatcact gttgattttg atgatgtggt
     2521 gattcgcggc gtgactgtga tccgtgcggg cgaaattacc tggccggcac cgccgattca
     2581 ggtatcagct cagccgcagg tggcacaaaa agcggcaccg gaagtgaaaa ctgaggaaaa
     2641 atgtgcctgt tcaccgtggc gtaaatacgc gttgatggcg ctggcaatca tccttttcgg
     2701 ctggctggca agcgttgcgc cgaaagagtt tcttgggcac ttcaccgttt tcgcgctggc
     2761 ctgcgttgtc ggttattacg tggtgtggaa cgtatcgcac gcgctgcata caccgttgat
     2821 gtcggtcacc aacgcgattt cagggattat tgttgtcgga gcactgttgc agattggcca
     2881 gggcggctgg gttagcttcc ttagttttat cgcggtgctt atagccagca ttaatatttt
     2941 cggtggcttc accgtgactc agcgcatgct gaaaatgttc cgcaaaaatt aaggggtaac
     3001 atatgtctgg aggattagtt acagctgcat acattgttgc cgcgatcctg tttatcttca
     3061 gtctggccgg gctttcgaaa catgaaacgt ctcgccaggg taacaacttc ggtatcgccg
     3121 ggatggcgat tgcgctaatc gccaccattt ttggaccgga tacgggtaac gttggctgga
     3181 tcttgctggc gatggtcatt ggtggggcaa ttggtatccg tctggcgaag aaagttgaaa
     3241 tgaccgaaat gccagaactg gtggcgatcc tgcatagctt cgtgggtctg gcggcagtgc
     3301 tggttggctt taacagctat ctgcatcatg acgcgggaat ggcaccgatt ctggtcaata
     3361 ttcacctgac ggaagtgttt ctcggtatct tcatcggggc ggtaacgttc acgggttcgg
     3421 tggtggcgtt cggcaaactg tgtggcaaaa tttcgtctaa accgttgatg ctgccaaacc
     3481 gtcacaaaat gaacctggca gctctggtcg tttccttcct gctgctgatt gtatttgttc
     3541 gcacggatag cgtcggcctg caagtgctgg cattgctaat aatgaccgcc attgcgctgg
     3601 tatttggctg gcatttagtc gcctccatcg gtggtgcaga tatgccggtg gtggtgtcga
     3661 tgctgaactc gtactccggc tgggcggctg cggctgcggg ctttatgctc agcaacgacc
     3721 tgctgattgt gaccggtgcg ctggtcggtt cctcgggggc tatcctttct tacattatgt
     3781 gtaaggcgat gaaccgttcc tttatcagcg ttattgcggg tggtttcggc accgacggct
     3841 cttctactgg cgatgatcag gaagtgggtg agcaccgcga aatcaccgca gaagagacag
     3901 cggaactgtt gaaaaactcc cattcagtga tcattactcc ggggtacggc atggcagtcg
     3961 cgcaggcgca atatcctgtc gctgaaatta ccgagaaact gcgcgctcgt ggtatcaacg
     4021 tgcgtttcgg tatccacccg gttgcggggc gtttgcctgg acatatgaac gtattgctgg
     4081 ctgaagcaaa agtaccgtat gacatcgtgc tggaaatgga cgagatcaac gatgactttg
     4141 ctgataccga taccgtactg gtgattggtg ctaacgatac ggttaacccg gcggcgcagg
     4201 atgatccgaa gagtccgatt gctggtatgc ctgtgctgga agtgtggaaa gcgcagaacg
     4261 tgattgtctt taaacgttcg atgaacactg gctatgctgg tgtgcaaaac ccgctgttct
     4321 tcaaggaaaa cacccacatg ctgtttggtg acgccaaagc cagcgtggat gcaatcctga
     4381 aagctctgta accctgacgg cctctgcgga ggccgtcact ctttattgag atcgcttaac
     4441 agaacggcga tgctttgacc tcccgctgtt tgttcaagcg caattttgac aataattgtt
     4501 aacggcacgg aaagcagcat acccaccggt cctaacaacc atccccaaaa aatcaacgac
     4561 aaaaatacca ccaatgtgga aagtcccagc ccacgcccca tgatgcgtgg ctccagaata
     4621 ttgccgaaga ccagattaat cagcagatat cccgccagca ccaacaacgc ttcgtagaag
     4681 ccattaaaca ccagtacctg agcaataggg gggattgccg cgagaactga accaatatta
     4741 gggatgtaat taagcgcaaa ggccagcaat ccccagacaa aagcgaagcg aacatcgagg
     4801 gcggcgagca tcgcccatgc gaccaggccg gtgatgatgc tgatggctgt tttcagcacc
     4861 agataatgag acacactgtc aatcgcacgc tgaatcgccg ccatcccttc aaccggacgc
     4921 gccatcattt gcttaaattt tccgggcaat tgtggcactt cgagcagcat aaacagcacc
     4981 gtcagcagca ataaaaatat tgatgacatg gcattagata actgcgtcaa taagttggtg
     5041 agcaacgtca tcgccgcgtt cggatcaatg taatgcgcca gttggtcaac tgagacgtca
     5101 atccctacgc gttgcaacaa cggctcaaga gcttgcagcg gcgtcataat agagttgcga
     5161 tattgcggta acgtccgcgt caactcgttg agcgtggaac ccagataagc taacagcaac
     5221 accatagcca tcacgatgat ggtcatcaaa atcgacactg ccagtacacg cggcacacgc
     5281 cagcggacca tgtgttgcac cagcgggtta agaataacag caataaataa tgcgagaata
     5341 aacggcacga tgatctcggc ggcaaaacgg ataccgcaga gaataatgac cagcattccc
     5401 aacataatga cgatttttag gccattgagc gtgatgagcg gctttgccat gcttgtcccc
     5461 gttttttctt cctttgctaa taataatgtc tttttcgctc cgtcatcata acgaaaatca
     5521 aaccgctcag gtaaagaagt gaaaatattt tgagttaatt cttaagctat gatacaaatc
     5581 aggcgtgttc aactaccgag gacaattatc atccgcgatg acgagaag
//
LOCUS       DBFIFS010000256         5611 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.256, whole genome shotgun sequence.
ACCESSION   DBFIFS010000256 DBFIFS010000000
VERSION     DBFIFS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.256"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1052)
                     /gene="dinB"
                     /locus_tag="N7A07_004271"
     CDS             complement(<1..1052)
                     /gene="dinB"
                     /locus_tag="N7A07_004271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU2478810.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLG"
     gene            complement(1123..1908)
                     /gene="lafU"
                     /locus_tag="N7A07_004272"
     CDS             complement(1123..1908)
                     /gene="lafU"
                     /locus_tag="N7A07_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU2478811.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            1880..3592
                     /locus_tag="N7A07_004273"
                     /pseudo
     CDS             1880..3592
                     /locus_tag="N7A07_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(3737..4234)
                     /gene="rayT"
                     /locus_tag="N7A07_004274"
                     /pseudo
     CDS             complement(3737..4234)
                     /gene="rayT"
                     /locus_tag="N7A07_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(4410..5159)
                     /gene="yafL"
                     /locus_tag="N7A07_004275"
     CDS             complement(4410..5159)
                     /gene="yafL"
                     /locus_tag="N7A07_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2478812.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            5369..>5611
                     /gene="dinJ"
                     /locus_tag="N7A07_004276"
     CDS             5369..>5611
                     /gene="dinJ"
                     /locus_tag="N7A07_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU2478813.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLF"
BASE COUNT         1305 a         1492 c         1491 g         1323 t
ORIGIN      
        1 aatcccagca ccagttgtct ttccatttga gggtcaagca acgtcacatg cagccccacc
       61 agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt ggcgattaag
      121 tcagctttat tcagccgcgg ccagacgtgc tcctgggtgg tttgctgaaa atcgtcgaac
      181 tttaatttca ccccctggcg agcaatcagt aaatcaggtt ttacctttgc cagacggcgt
      241 tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg atgaatatct
      301 tccgccatcg tgcgttccac gccgacggat tttcgtaacc gttcgctgtt aacgtcgcgc
      361 tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa gcgtttaagc
      421 agaatcacca gatcacactt ttgtacatca ccgcaggtcc gcagccccat cgcttccagc
      481 tttgccgctg agactttgcc aacaccggga atttttgcca gcggtaaggt ttgcagaaat
      541 gccgaaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat gtcggaggcg
      601 attttggcga gaaactttac tggtgccacg cccgcagacg ccgtcagttg cagctcgctg
      661 aaaattgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca atggacgcta
      721 tcagtgacat cgaggtaggc ttcatccagt gacaacggtt caatacgcga ggtgtagcgc
      781 gagaagattt cacggatatg atttgaggct tctttgtagg cgtcaaagcg ccccggaagc
      841 aaggtgagat gtgggcataa tttgagcgcc atccctgtcg gcatagcgct gcgtacgcca
      901 aatttacgcg cgggataatt ggcggtgctg atcacccccc gacgttcgcg gctgccgcca
      961 atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccactgc ggcgaaaaag
     1021 cagtccatat ccacatgaat gattttacgc attactcacc tctcaatact ggtaaagtat
     1081 acagtgattt cagggtttga gaaatgcgta aagattcagc atttagcgcg ttcgctgcga
     1141 aagcacctgc tgcttatcca gcttttgcac cagcggctgc accactttat ccccatgctg
     1201 accaaagtat tgatacagcg tatcggacgc acttttggtc agcaccataa tctcaatgcg
     1261 tcggttgccc gcgctttgcg gatttttcgc gtccagcagc atctggtccg ccattgcgct
     1321 tacctgcatc actttatctt ccggcattcc ggcctcttcc agcacccgac gagctgaaag
     1381 cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg tttttgtagg ccatcgcatc
     1441 ggtatgcccg gtaataataa ttttgttatc gagcgagtcg aacactggtg cgagctccac
     1501 cagcaaagtt ttaaagaacg gcataatctg cgcgctgccg cgttcgaaca tattgcgatt
     1561 ctggtcgtct ttaatcagca cgcgtaatcc ctgcgggaca atctccatct ccagattcgc
     1621 ttccatatgc gcatcacggg caatggtgtt gatactggtt gccagctcgc caagctcggt
     1681 ggcggatttc ttcttcagca gcgccgtttt ctcattcaca tcacgcgctt ttttctccgt
     1741 ctcttccggc accgccacgg tggcaggttt tggcggatga gacgggctga ttttatttaa
     1801 cggcgacagc ccaccaccgt taaaaatcga ctgcccgtgc agcgcggcga taatgctttc
     1861 acgctcggat ttactgacgc tgttgacaat caactttatc gtcgtcacca aaggggccga
     1921 gcgtatttcc gaggtttctg cccgctttac cctggatgcg atgcccggca aacagatggc
     1981 gattgacgcc gatcttaacg ccggattgat caaccaggca caggcgcaaa cccggcgtaa
     2041 agatgttgcc agcgaggccg atttctacgg cgcgatggac ggggcatcga agtttgtgcg
     2101 cggggatgcc atcgccggga tgatgattct ggcgattaac ctgatcggcg gcgtctgtat
     2161 cgggatattt aaatacaacc tgagcgccga cgccgccttc cagcaatatg tgctgatgac
     2221 catcggcgat gggctggtgg cgcagatccc ttccctcctg ctctccaccg cagcggcgat
     2281 tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat gacgtgcgcc accagctgct
     2341 ggcaagcccg tcggtgctct acaccgccac cgggattatg tttgtgctgg cggtggtgcc
     2401 gggaatgccg cacctgccgt ttttgctgtt cagcgccctg ctgggtttta ccggctggcg
     2461 gatgagcaaa cagccgcagg cggcggaggc ggaagagaaa agcctcgaaa cgctgacccg
     2521 caccatcact gaaaccagcg agcaacaggt cagttgggaa accattccgc ttatcgagcc
     2581 tatcagttta agcctcggct acaagctggt ggcgctggtc gataaagcgc agggcaaccc
     2641 gctcacccag cgtattcgcg gcgtacggca ggtgatatcc gacggtaacg gcgtgctgct
     2701 gccggagatc cgcattcggg aaaacttccg cctcaagccc agccagtacg ccattttcat
     2761 caacggcatt aaggccgatg aagcggatat tccggcggat aaactgatgg cgctgccctc
     2821 cagcgaaacc tacggcgaga ttgacggcgt gctggggaac gacccggcgt acgggatgcc
     2881 ggtgacctgg atccagcccg cgcagaaggc gaaggcgctg aatatggggt atcaggtgat
     2941 cgacagcgcc agcgtgattg ccacgcatgt gaacaagatt gtgcgcagct atattcctga
     3001 tttgtttaat tatgatgaca tcacgcagct gcataaccgc ctgtcgtcga tggccccgcg
     3061 cctggcggaa gatttgagcg cggcgctgaa ttacagccag ttgctgaaag tgtaccgtgc
     3121 gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc atcgccaccg tgctggtcgc
     3181 cagtagcgcg gtgactaaag atcatattct gctggcggcc gatgtgcgcc tggcgctgcg
     3241 gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg acggtgtata cgctgaataa
     3301 tgagctggaa aatctgctga ctaacgtagt gaatcaggcg caacagggcg gtaaagtgat
     3361 gctcgacagc gtgccagtgg acccgaatat gctcaaccag ttccagagca cgatgccgca
     3421 ggtgaaagag cagatgaaag cggcggggaa agacccggtg ctgctggtgc cgccgcagct
     3481 gcgccctttg ctggcgcgct atgcaaggtt gtttgcgccg gggctgcatg tgctgtcgta
     3541 taacgaagtg ccggatgagc tggagttgaa gataatgggg gcgttgagtt gattggcctt
     3601 taaggggcgc atccggcatt ggtgcacaat tgccggatgc ggcgtgaacg ccttatccgg
     3661 cctacggttg agtgcagttt tgtaggcatg ataagacgcg ccagcgtcgc atcaggcatc
     3721 cggcaccagc gcacaattat gaaatgatac gctccccggc actaaaatcc gttatgtctc
     3781 ccgcccaatc aatgggatat aaccctcgcg ccacatcgcg atggaacgtt gagaatggcc
     3841 aatcactcac ttgctttacc cagccatgtt ttactggatt tatataaata taatcaacat
     3901 gatggcgata gtctttggta ttgcggatgg tgtgctccta aaaacgtggt tgccagatat
     3961 ttttcaatcc acaagcatgg gtgaactgct ttttaatctc ccgccagcgg gaggaaaaat
     4021 catcatcgct ttcgggtaat gtccatatgc aatgcatatg ctccggcaaa acgacccagg
     4081 cgttaatttc aaaaggtctg tctcgcttaa cgttaataat ggcgttacgg agcatctgga
     4141 actgagcggt cagaaggtgg cttcgacgat ttcgtaggtt aaccgtgaaa aaccaggttc
     4201 cccctttgat gtaataccga cgatattcag acatggcaac tcctttttct caacggtaag
     4261 aagaaaagga aaggtcggca atgaggtgat ggaataacgg gaaagcggct cgcaaaaacg
     4321 cacagatttg taggcatgat aagacgcgtc agcgtcgtat caggcatctg cgcacaactg
     4381 ccggatgcgg cgtgaatgcc ttatccgtcc tacaaaatcg tctcttccgt caaaatccgg
     4441 cgcgcgccta aaaagtgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
     4501 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
     4561 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
     4621 gccacaatcg ttgcccgatg gtagtgatac atctcattag ccgtacgcgg gagcttcgcc
     4681 tcgaggatct tgttgtaggc ataaaaaacc aacccgctac agtcaaagcc tttatcaggc
     4741 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccaggcggtg aatcgccact
     4801 tcggtaatgt tatgcagatg gtcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
     4861 cagcgtctgt cactcgcctt gagcggtgca ggaaaccatt ctggatgctg ttttatctgt
     4921 tcgcggttgt gctggcgtag cgcccttctg ctttcggcat tgccttccac aatatagctg
     4981 acctgctttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
     5041 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgctggcaaa tgaagaaaac
     5101 ggcaaacaaa tcaacagtaa tcccgacaat acaaaggatg gaatcgacgg caaggacata
     5161 ctcaataatg gctggtcatg cattgatgtt acagtgtatc ccggcccgta cctgaacaaa
     5221 cttaataaac cattattata aaattaataa atatatttca gttaattatg taaaaacatc
     5281 cgtttattaa attactcata gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     5341 ataaatatac agcataggag atatcccaat ggctgctaac gcatttgttc gcgcccgaat
     5401 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     5461 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     5521 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     5581 tcataaggcc aaagacgccg atgatttatt t
//
LOCUS       DBFIFS010000257         5470 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.257, whole genome shotgun sequence.
ACCESSION   DBFIFS010000257 DBFIFS010000000
VERSION     DBFIFS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.257"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..585
                     /locus_tag="N7A07_004277"
     CDS             <1..585
                     /locus_tag="N7A07_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2478814.1"
                     /translation="KTIQLRNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAA
                     GQALRLLYTGNNLPQDRESVFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSI
                     VGKSSDAPKNLTYQVKQNRIEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTT
                     EWTLPSGISVAPGAQIHLVTVNDYGVNVTSEHAL"
     gene            638..3155
                     /locus_tag="N7A07_004278"
                     /pseudo
     CDS             638..3155
                     /locus_tag="N7A07_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3171..4505
                     /locus_tag="N7A07_004279"
     CDS             3171..4505
                     /locus_tag="N7A07_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478815.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            4502..5191
                     /locus_tag="N7A07_004280"
     CDS             4502..5191
                     /locus_tag="N7A07_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2478816.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(5337..>5470)
                     /locus_tag="N7A07_004281"
     CDS             complement(5337..>5470)
                     /locus_tag="N7A07_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2478817.1"
                     /translation="GRIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1452 a         1363 c         1336 g         1319 t
ORIGIN      
        1 aaaaccatcc agttgcgaaa taccagcgat cagccctata tcattaatat ccatgttgag
       61 gatgaacgtg gttctgacaa gaatgtaccg tttatgccaa ccccgcagac atttcgcatg
      121 gaagctgccg caggtcaggc gttacgcctg ctctacactg gtaataattt accgcaggat
      181 cgcgagtctg ttttctggtt tagtttcagt caactacctt atctgaataa gaatgataaa
      241 agtcagaacc agctcatcct ggccctgact aatcgagtca aaattttcta tcgtcccagc
      301 tcgattgtcg gtaaatccag tgacgcaccc aaaaacctga cttaccaggt aaaacagaac
      361 cgcattgaag tgacgaatcc cacgggctat tacgtcacaa ttcgcgccgc tgaactgctt
      421 aataatggta aaaaagtccc cctcgcgaat tcggtaatga ttgctcctca aagcacaact
      481 gaatggacac taccctctgg catcagtgtc gctcccggtg cgcagatcca tttagtgacc
      541 gtcaacgact atggcgtaaa tgttacgtct gagcatgcct tataacctct ttctcctttt
      601 ttgagcggta tcctttttca cgcacgagta cgtcgagatg aataaatccg ttgtgtcaat
      661 ttctgcggca atgttggttt tactttgcca accggtcatg gggagcgaaa tctcacccgc
      721 aacaccgtca gatgaagaca actacacctt tgacccgcaa ctcttccgcg gcagcagatt
      781 tagtcagtcg tcattagcaa aactgacaac acgtgagtct gttgcaccgg gcaattataa
      841 aatggatatc tacaccaaca ataagttgtc aggcagttgg aatgtcacgt ttaaagaagc
      901 cgctgatggt cgcgttctgc cctgcctgac gcctgaagtc gcggacgcga taggcctcaa
      961 aacaggggaa gataaggggg aaaaagatcc tgtctgtacg tttgctaagg aactcgctcc
     1021 cggcatcacc agccagacac agttgtcaca attgcgcctg gacttatcgg tgccacagag
     1081 tcaattgatt agtcgccctc gcggctatgt tccccccagc gagctggata ccggagcatc
     1141 gctggcgttc atgaattata ttgccaacta ttacaacgtt gcctattcag ggcagaatgc
     1201 tcatagccag cgttcgctat gggcatcatt taatggtggc atcaaccttg gtgcctggca
     1261 atatcgtcag ttatccaaca tgacctggga taatgacaaa gggaatcagt ggaacaatat
     1321 tcgtagctat ttgcaacgcc cgctgcccgc cataaatagc cagttaatga tggggcagct
     1381 tatcaccagc ggaagatttt tctctggact cagttatcac ggcgttagtc tcgcgaccga
     1441 tgaacgtatg ctgccggact ccatgcgcgg ctatgcgccg actattcgcg gcgtggccgc
     1501 aacaaacgcc agagtctcgg taatgcaaaa cggtcatgaa atatatcaga ccaccgtggc
     1561 tcctggccct ttcgagataa acgacctata ccccaccagc tacagcggcg atctggatgt
     1621 caccgttacg gaagctaacg gcgcagtcag tcgtttcagt gtcccctttt cagccgtacc
     1681 agaatcgatg cgtccaggaa cttcccgtta taacgtggaa gtaggtaaaa cgcaggatag
     1741 tggtgatgac tcgatgtttg gtgaccttac ctggcagcac gggatgacta atacgctgac
     1801 atttaacagt ggttcgcgta tcgctgatgg ctaccaggcg ctgatgctgg gcggagtcta
     1861 tggcagttcg ctgggggcat ttggggcaaa cctcacttgg tcccatgcgc gtgttcccga
     1921 aagcgaagcg cagagtggtt ggatgtcgca attaacctgg agtaaaactt tccagcctac
     1981 ttcaaccacc gtctccctgg caggttatcg atactctacc agcggctatc gtgatctggc
     2041 tgatgtgctg ggagagcgtc atgctgccag caataaacag tcatgggact ccagccagtg
     2101 gcgtcaacag tcgcgcttcg atcttacgtt aagtcagagc cttgcgaatt acggcaacct
     2161 gtttgtgtca ggttcaacac agaactaccg tggcggcaag agccgtgata cacagcttca
     2221 gttaggttac agcaatagct ttagccatgg catcagtatg aaccgtcgga cgccaaagaa
     2281 tgggcggcta taaagacaat tctgatgata tgcagacggt aacatccctt tcattctcat
     2341 tcccacttgg cggcaatgga cctcgtgtac caagtcttag caacagctgg acccattcaa
     2401 ctgacggtag ctcgcaatta caaagctcgc taaccggaat gcttgatgaa gcacagacca
     2461 ccaactacag cctgaacgtc atgcgcgatc aacaatataa gcagacgacg cttagcggaa
     2521 acatgcaaaa acgtttttca caaactaccg tcggattgaa cgcatcgaag ggccaggatt
     2581 actggcaggc ttcaggtaac gtacaaggcg cgatggctgt gcatggtggc ggcattactt
     2641 tcggacctta tctgggtgaa acgttcgccc tggtcgaagc taaaggcgca gaaggtgcaa
     2701 aagtctataa ctccagtcag ctggaaatta atgacagtgg ctatgcgctt gttccggcag
     2761 taacgcccta tcgctacaac cgtatatctc tcgatccaca aggaatggat ggcgatgccg
     2821 agttggtcga cagtgaaaga caggtagcac cggttgcggg tgcggcggtg aaagtaattt
     2881 tccgtacccg tcctggtaaa gcgttgctga ttaaatcccg catggcagat ggttcggaac
     2941 tgccaatggg agccgatgtg ctggatgaga ataatacagt cgtcggtata gccggtcagg
     3001 gggggcaaat ttacctccgc acagaacaga caaaaggcca cttgtcagtt cgctggggtg
     3061 aaggtgctaa cgatagctgc caattgccct ttgatatcag cgggaaggac agcaatagcc
     3121 ctatcatccg cctgaatgaa acctgtcagt cttgatacag agaaaacccg atgtctaaat
     3181 cgactttttt acacattctg attagctcca tcatattggt ggcactaata caatcatccg
     3241 cttgggctaa ctgtacaaac acacagatag gtcaaactga agatggtcgg acagcgctta
     3301 ttgagttcgg aaaaattaat atgaccgaca cctattttgc gccagcaggt tcactcctcg
     3361 cgacaacggt cgtacctccc actaactaca catcaggtgg cgcgacagga agctccgtat
     3421 tgtgggaatg tgatgcaaca gatttgccaa acatctattt tctggtcgct accaatggcg
     3481 acgaccgcgt ggggggcttt tacgatgcag gcgggcctga tggtctgagt gatgtctatg
     3541 ccacctggtt cgcttttgtc ggtctcaagc agaccatggc gggcgtgacg cttggtcgtt
     3601 actggaagaa agtgcccatc accagttatg ccactcaggg aactaaaatc cagattcgct
     3661 tacaggatat ccctcctctt catgctgagc tttatcgcat cagtacgcta cctgatacat
     3721 cagcaacaac aagttggtgc ggtaataata atacagatag tagtggagtc ggattcgcaa
     3781 aaccttccgg tacaatctat aactgtgttc agcccaatgc ctatattcag ctttccggta
     3841 ccagcggcat tttatttggt catgatgagc ccggcgaaga tagttctgtt cattgggatt
     3901 tctggggtgc tgataatggt tttggttacg gaatgcgttc ggccaatcga ctctacaaca
     3961 atgccacctg cgttgcccgc agcgccacgc cgttagtatt gctgccgaca attgcagaag
     4021 cacaactgaa tgcgggcatg gaaagtaccg gtaattttaa tgtccgcgtc gagtgtagta
     4081 actcggttca atcagggatt agcgatactc agacagcatt aggaatccag gtgtctgaag
     4141 gtgcatatac agcggcgcaa aaactgggga ttatcaatag caacggcggc gtcagcgccc
     4201 tggtctctga taattatgac gcagcagaga tggcaaaggg cgttgggatc tacatttcta
     4261 acagtgctca ccccgatacg gcgatgacgc tggttggtca accgggcatc gcgaagttaa
     4321 cccccggagg aaatgcagcg gggtggtatc ctgtatttga aggggcaaca ttagaaggtg
     4381 cgactcaccc cggatactcc agctatagtt actcttttat cgcccggttg aagaaactgc
     4441 caaatcagac agtcagtgcg ggaaaagtgc gggcaacggc ttatatattg gtgaaaatgc
     4501 aatgaaatcc gttttgctgc tcttaaccat cttgttcgta cagtcagtgg aagccggcat
     4561 catgccggct tcttcacgga ttatttatcg cgatggcgat cgggaaaaga cactgatgct
     4621 ggtcaatacc aatgactatc cggtactggt gcaaagttgg gtagataatg gtgaaggttc
     4681 cccagaagct gcttctcccc cctttgtggt gctgccaccg gttttccgcc tggctgcgag
     4741 acgaacccag gggttgcgga ttatctataa tcatgaccca cttccccagg atcgcgaatc
     4801 tgcattctgg ttaaatcttt atgaaatgcc gccagaaact ccccatcaag atcccacaca
     4861 tttaacgctg gcaatgaata cgcaacttaa aatcttttac aggccaaaat ctgtaactat
     4921 gacgccccaa gaagctatgc gtaaaataag ttggcatctg ggtaacgacg gcagcagtag
     4981 ctatattgag cttaataacc caacaccact gaatatctct ttcacctcca tcagcattgg
     5041 ggctacaaag gtgcagcaag aaagcgatat gatgataaaa cccttcagtc aacgacggta
     5101 ctggttagca agtaataatg ttagtggtgt ggttgcggcg acatatctcg atgataatgg
     5161 tgcacagata aaacaacaat tagcgcaata atgctctgag tggtatgtaa actcatcgcg
     5221 atggttcaac gctaacgaat aaccactata cccacctgaa gtaccggaag tggagaaatg
     5281 acaaaatctc tccactccat tttccagtca aaacacctca acgcagccaa aaatcgttaa
     5341 cgaattgaat atccaccatc aatgactaat agttgtccat tgacataatc tgaagcggca
     5401 ctggcgagga acaccattgc gcccataaga tcctgtgccc ttccccagcg ttgagccggt
     5461 atacgccctt
//
LOCUS       DBFIFS010000258         5430 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.258, whole genome shotgun sequence.
ACCESSION   DBFIFS010000258 DBFIFS010000000
VERSION     DBFIFS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.258"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..384
                     /locus_tag="N7A07_004282"
     CDS             <1..384
                     /locus_tag="N7A07_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2478818.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="N7A07_004283"
     CDS             419..1117
                     /locus_tag="N7A07_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2478819.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="N7A07_004284"
     CDS             1139..1363
                     /locus_tag="N7A07_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478820.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="N7A07_004285"
     CDS             1360..1716
                     /locus_tag="N7A07_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HGU2478821.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="N7A07_004286"
     CDS             1749..1901
                     /locus_tag="N7A07_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478822.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="N7A07_004287"
     CDS             1898..2209
                     /locus_tag="N7A07_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478823.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="N7A07_004288"
     CDS             2336..2899
                     /locus_tag="N7A07_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478824.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="N7A07_004289"
     CDS             3009..3113
                     /locus_tag="N7A07_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478825.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="N7A07_004290"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="N7A07_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2478826.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="N7A07_004291"
     CDS             3680..3958
                     /locus_tag="N7A07_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478827.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="N7A07_004292"
     CDS             3960..5009
                     /locus_tag="N7A07_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2478828.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            5022..5381
                     /locus_tag="N7A07_004293"
     CDS             5022..5381
                     /locus_tag="N7A07_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2478829.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVPGERLGIKITELECA"
BASE COUNT         1444 a         1225 c         1521 g         1240 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggtcacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 taccttaccc gccgacggtg aacacctact ggcgacgtcg tggcagcaca tattttgtat
     5101 caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag cagcggctga
     5161 aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg gataagcgcc
     5221 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
     5281 taatggacga tgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg
     5341 agcggctggg gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt
     5401 tgtgcgtgag ctgctcatta tcgctaccgc
//
LOCUS       DBFIFS010000259         5324 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.259, whole genome shotgun sequence.
ACCESSION   DBFIFS010000259 DBFIFS010000000
VERSION     DBFIFS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.259"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..83
                     /locus_tag="N7A07_004294"
     CDS             <1..83
                     /locus_tag="N7A07_004294"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU2478830.1"
                     /translation="NVINEETLLQQPEERFAWLRSKKDRF"
     gene            155..1513
                     /gene="mltD"
                     /locus_tag="N7A07_004295"
     CDS             155..1513
                     /gene="mltD"
                     /locus_tag="N7A07_004295"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU2478831.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(1561..2331)
                     /locus_tag="N7A07_004296"
     CDS             complement(1561..2331)
                     /locus_tag="N7A07_004296"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2478832.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2409..3209)
                     /gene="yafD"
                     /locus_tag="N7A07_004297"
     CDS             complement(2409..3209)
                     /gene="yafD"
                     /locus_tag="N7A07_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU2478833.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3450..4364
                     /gene="yafC"
                     /locus_tag="N7A07_004298"
     CDS             3450..4364
                     /gene="yafC"
                     /locus_tag="N7A07_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU2478834.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4361..5164)
                     /gene="dkgB"
                     /locus_tag="N7A07_004299"
     CDS             complement(4361..5164)
                     /gene="dkgB"
                     /locus_tag="N7A07_004299"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU2478835.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1401 a         1274 c         1323 g         1326 t
ORIGIN      
        1 ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa
       61 ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg
      121 atcggtcgtc ttttaagcaa ctattgacgc acacatgaag gcaaaagcga tattactcgc
      181 ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct
      241 ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga
      301 tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat
      361 gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag
      421 ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag ccgggcaagt
      481 taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga
      541 tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg
      601 gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc
      661 aacaactgcc gcgctgaata tgatgcagcg tctgaacaaa atgtttgatg gcgactggct
      721 tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa
      781 caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca
      841 gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt
      901 acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gtagcccggt
      961 tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa
     1021 cgctggcgtg aaaggctcca cgctgggcgc aagtggtccg cagtacgtga tggtgccaaa
     1081 gaagcatgca gatcaactgc gtgaatctct ggcttcaggc gaaattgctg ctgtacagtc
     1141 gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga
     1201 cacgctttca agtatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa
     1261 caaactgcgc ggctctaagc tgaaaccagg ccaaagtctg acgattggcg caggtagtag
     1321 cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc
     1381 gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag
     1441 cgatactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacagcat
     1501 gccagactcc tgacaaacca gataatagaa aggcaccgat tcccccggtg ccttttttat
     1561 ttatgccgct ttatgtgcat ctaccatgat gatatcactg gtgaaagagc catcattctg
     1621 caaggcaaaa tacgttctca cctctgtcga tgcgctctgt tggtaaatgc gaatagcgtc
     1681 tactaacgct tctggcgtac gcattctcgc gacccatgaa gaaaattcca gcggtaactt
     1741 atctgtaatt aaattatcaa ctatcagatt ggcttcattg attaacctca accactcacc
     1801 gctggcgtag tttcgtacgt gagaggtatc gcataatgct tctaccgtct gtaaccagat
     1861 gtcgcgcact gggtggcccg gagacattac gtccatcaca atcagcctac cgccaggttt
     1921 caatatccgg ttcacttcac gcagtgctgc accaacatca tgccagtgat gggcagaata
     1981 acggctgata acaatatcaa atgcgttatc ggcaaatggc agactttcgg catacccctg
     2041 gcgggtggtg atatttttca gttgccggac ttcggcagct tgtgccacga catccagcat
     2101 ttgggcagat aagtcatacg ccaccaccgt gctcacattt tgcgcagcga caaagctggc
     2161 atgccctgct ccgcagccca tatcaagtac acttgcatca ggataatcag ccagacacac
     2221 cgccaggagc tgtaaatctc gtccggatgc atgtacggta ctggtcagat attcgctggc
     2281 ttgagagcta aactgctttt ctacgtggtc atggtgggat tgtgttgtca ttgcactgtc
     2341 cttttgttgt ttgagtataa aagggcagct ccaaacgttg gtctgccctg tggcagacct
     2401 gacatacctt atttatcagg cttgccggga ctgaattcaa cgagtagcgg attgtgatcg
     2461 gacgcgcgcg taaccagtac agaagcttcg ctgacgttca gaccacggta gaaaacaaaa
     2521 tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac ctgacgcagc
     2581 gacatttccc gcgcaaaacg atataacgcg ttcatccttc tacggctcca agcattgaaa
     2641 tctcccgcca taatgaccgg gccgctgtgg tgagctatct gatcaccaat aggaagtaac
     2701 tgcttactat aaacatccac gcccagactg aagttgacgg cgtgtatatt aaccaccatt
     2761 aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa
     2821 atgggttctc gttcacgtaa cgggcagcaa tacactggat gtgccgccga aagggtcatt
     2881 acgccagaag gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc aagatagtta
     2941 gcagtcgcaa actgtaccaa ctctggcgtt gtttgcgctt cctgcaataa caccagatgt
     3001 gcgtctttgc cgtaattctt taataccgac aaccattcag cgcgttgctg tttgtatatg
     3061 ttccacacca ggatccgaat acgctcttcg gtacttaacg gttctccggg tggtaatgcc
     3121 tggccgatgc tcgcaaaaga ccccggcggt aagatcctct ccgcaggttg tccggcaaca
     3181 tagcgcatgg cataggtgtt ttttcgcact tttgactttg aaacctctat tacgtcaacc
     3241 cttccgcact cgggagggtt ctactgttat agggatttta gctcactctt tcaacgagca
     3301 attgtatatt gttatgtaag caagtgctta caccggtcag aattttgctc attattgaga
     3361 acattaaatc ggatgacata tcacgcatcc tgatggttta atgacaaata aattcaaaat
     3421 atctcatcta ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt
     3481 ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta gggcaagcaa
     3541 actcagcggt aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt agcctgctta
     3601 atcggaccac gcggcaactt agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac
     3661 agtcaatttt gcaggagatg gcagcggcag aatcagaaat tatggagacg cgtaatacac
     3721 cgcgtggact gttgcgcatc gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt
     3781 tgattaagcc ttttcgtgaa cgctatccgg aagtcacttt atcgctagtc tcctccgaaa
     3841 cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat acgcgccggt acgttaacgg
     3901 attccagctt acgtgccagg ccgttattta acagttatcg aaaaattatc gcctcccccg
     3961 attatatttc ccgctacggg aagccagaaa cgatcgacga tttaaagcaa catgtttgcc
     4021 tgggattcac tgaacccgct tccctcaata cctggccggt agcctgtagc gatggacaat
     4081 tacatgaggt gaagtacggt ttgtcatcca atagtgggga aacactgaaa cagctttgcc
     4141 tgagtggcaa cgggattgcg tgtttgtccg actatatgat cgacagagaa atcgctcgcg
     4201 gagaattggt ggagttaatg gcagataaag tgttgccagt ggaaatgcca ttcagtgccg
     4261 tctattacag cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat ttccttagcg
     4321 agcatgtaaa aacagctccc ggaggagctg tcagagaggc ttaatcccat tcaggagcca
     4381 gaccttccgg gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct tttttatctt
     4441 cggcatcaag ctgtaaattt tgtgccttaa gattactctc caggttttca cgtttagtag
     4501 aagaaggaat tactgagtaa ccttccccca tagcccacgc cagaatcact tgtgccggag
     4561 tcgcattgtg tttagctgcg atacgagcaa taacctcatc tttcagggcc ttaccatacg
     4621 ccagcgtcat ataggaagta atatggatgc cgtgctgttt agcccaggca accactttac
     4681 ggttttgcag ataaggagag agttcaatct ggttagtagc gatgttttca gcgccaacag
     4741 cagcaatcgc cttttccatc aatgggatcg tgaagttgga aataccgatc tcacgcgtca
     4801 acccttcttt tttggcttcc agcagcgcct gcataaactc ttcaacagag acttcatcgt
     4861 ttggtgacgg ccagtggatt agcgtcagat caacataatc ggtacgcaat ttttgcaggc
     4921 tctcttttag actcgggatc aatttgtctt tgctgagatt ttcaatccag attttagtgg
     4981 tgatgtagag ttcatgacgt ggcacgccac tttctgcaat cgccagacct actgcggctt
     5041 cgttatcata gatttgtgca gtatcaattg cgcgataacc aagttcaagc gccgttttca
     5101 cagatgaaat aacaacgtcg tctttcagac ggaaagtacc taaaccaaat gcagggatag
     5161 ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg gctttagaat
     5221 gttgcggaaa aagggcgtaa aacgcagagg atttttgcga ttttagcaat aatagaaatg
     5281 tagacgaaaa aaaacctcga gcgataactc gaggcttctt aata
//
LOCUS       DBFIFS010000260         5316 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.260, whole genome shotgun sequence.
ACCESSION   DBFIFS010000260 DBFIFS010000000
VERSION     DBFIFS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.260"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..541)
                     /locus_tag="N7A07_004300"
     CDS             complement(<1..541)
                     /locus_tag="N7A07_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU2478836.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
     gene            complement(538..1143)
                     /locus_tag="N7A07_004301"
     CDS             complement(538..1143)
                     /locus_tag="N7A07_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2478837.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            complement(1143..1865)
                     /locus_tag="N7A07_004302"
     CDS             complement(1143..1865)
                     /locus_tag="N7A07_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU2478838.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(1940..2620)
                     /locus_tag="N7A07_004303"
     CDS             complement(1940..2620)
                     /locus_tag="N7A07_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2478839.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVWLWDYANRSQALFRELYPA
                     LKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKE
                     LPPSLHRY"
     gene            complement(2876..3634)
                     /locus_tag="N7A07_004304"
     CDS             complement(2876..3634)
                     /locus_tag="N7A07_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU2478840.1"
                     /translation="MTTKISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLRVTLSNSQNLQPSLRGL
                     QISPKARSLILWTERGAARHAKMLETDRAWEVFEKLEDCYFSQKIPEQLQLPESTLSI
                     NYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRILNELHSKGYNVDAAVAE
                     FNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(3909..4091)
                     /locus_tag="N7A07_004305"
     CDS             complement(3909..4091)
                     /locus_tag="N7A07_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2478841.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(4088..4615)
                     /locus_tag="N7A07_004306"
     CDS             complement(4088..4615)
                     /locus_tag="N7A07_004306"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU2478842.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGLGVRRAA"
     gene            complement(4612..5058)
                     /locus_tag="N7A07_004307"
     CDS             complement(4612..5058)
                     /locus_tag="N7A07_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU2478843.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            complement(5015..5251)
                     /locus_tag="N7A07_004308"
     CDS             complement(5015..5251)
                     /locus_tag="N7A07_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HGU2478844.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
BASE COUNT         1190 a         1326 c         1227 g         1573 t
ORIGIN      
        1 tgtccgcgcc tttgatttct ttcacgatcc cgtcttgtga gttgctgatt cgttcgcggt
       61 tccagattac ctgctgatga tcaactggct ttccaaactc gtatttatca caaggataat
      121 cggcgtggca gatgacatat ttttttcctt tactcaccag ttcgatgatt aacggaagtt
      181 catctgcttt atgggcaaga gctttagcca gaatttcttt gtcgtaatcg agattaaaga
      241 accagccacc gccattaagc agccagtgat tgacgtttcc acgctctgat aagccatcaa
      301 tcatcatttg ctcatggttt ccacgtacag ctctgaacca ggggaatgtg attaattcca
      361 ggcattctac gttctctgta ccgcgatcaa ccaaatcgcc caccgagata agcaggtctt
      421 ttttggtgtc gaatcctatc gtctccagtt ttttcatcag gttcgtgtag catccgtgca
      481 gatcgccaac tacccaaata tttcggtatt tgctgccatc aattctttcg tagatattca
      541 tgcaacctca cttctgctgt ttcgcagttt tttaagtttc tgttgatact ccgccttgat
      601 ggccctgcac tcttcgacag tccagcgatg gcggttatgg tttgattcga tttcgtctac
      661 tgcttcctgc ccgatgcggt taatcagttc gacgcgatac ggaacgagat ttccgctttt
      721 atgttggttg cacaccacgc attgcttgtg aatattgcgt tcatcaaatc ggagttgagg
      781 cgccgcagca gttgtccggt aatgtccggc atcccactga gcagacgtga gcgttccgca
      841 cgagatacat ggtaagtcgc ggtctctttc tctgatgaag gcgtttacgg cttgttgggc
      901 ttgtttaatc cagtaactgc ggggctttaa ggcgagtttt cgaatcttaa ttttatcttt
      961 ctgtttctgc tcctctcgtc gtcgtttctt ctctgctgtt ttttccgcct tttcgcgttc
     1021 tttatttcgt cgttcgagtg ctaatttagt tccgtgttcc gggctgcacc accactgatt
     1081 tgagaatgcc gggtgaaacc attccttaca gattttgcat ttccttcgcg ctggtttagc
     1141 cattaagcag cctcccctgt tactttaagc attccgttat ctagcagctt tcttgtcagc
     1201 cactgttgac cacgcccggt gatttttgtg gtgaacgata tctgtattcc gtgatttgta
     1261 ttgaccgctg tttctttcac tgtgaaatag ccgcgatcca tatattcctg cattggcaca
     1321 ttgcgccggg cacctgaagc aataaggatt ttgtgatcgc gcatccacgc aaaccgtttg
     1381 tttggaccaa taccaacaac ctttgcaaag tttccaatca aaattccgct ggcctcgcca
     1441 actcgatcgg caaactcaac tttaggtgct gcgagagcaa gctgtttctc cagttcagcc
     1501 ttctggtctt caaggtcggc cgcaaggcgc aatgcctcag aaaaggtttg tggtattttc
     1561 gcggttgccc cttcgagttc tcgccagcgg tcaacaaggc gagcggtgaa ttccggcgac
     1621 aactgagcga cgacaataat gctgtctcgc ttaccttgtt cgccttcgaa tatatacacg
     1681 cttgtgaatt tgttagggct aattgtttgt ttattttcaa ctttttgcat tgaaggaagt
     1741 tgaatcaccc cacgctttgc cagacgttct attgatattc tgacattacc gtgttggctt
     1801 cccaccaact cagcgatttc aatgcttgtc attttgatgg cattgctatt tatcagctca
     1861 ttcattgtca tgtcctctca cattgaaaat tcagcaataa aaaacccagc cgaagctggg
     1921 tttgttaagt tgtcaattgt cagtagcgat gcagtgaagg cggcaactct ttgttcttaa
     1981 gcctttccca tgccagaagg ttcgtcggcc cgtcaggctc ataaatatct atatcccgcg
     2041 tgtgattaat taaaacgccc ctcgccctcc cgatgatata cgagaactca tagccgtagt
     2101 cgtggcatat gccggaatag ccagactgaa tcagttttaa tgcgggatac aactcacgga
     2161 acaatgcctg tgagcggttg gcataatccc acagccatac aaggctgtct gtttcttttg
     2221 cggaaagccc gttgagcttc ttctcttgtt tgccagtatt tttctcgcac tggctgaaat
     2281 agcagtcttc cagtttttcg aacacttccc acgcccgatc ggtttcgagc atttttgcgt
     2341 gacgggctgc gccgcgttct gtccagagga tgagggagcg aacgttacgg gcgattttca
     2401 cagaaggtct taaagacatg ctgtgcttaa attcgcgcaa ctcctcattc tcaagtaaaa
     2461 agtagtgctt accgataaca aatcgtccag aattccgatt gaaatttttt cggatgtaat
     2521 ctggttctgt gccatataag ctggcaagaa gctctgtagt caccacaggt atattactaa
     2581 atgaaattat ggagagggac tccacggaga tggttttcat gctttatgtc ctacttgata
     2641 ttttgattta ccccttttga gagggtgacc gggcgctcaa aaccgcaagt agtcggcggg
     2701 catattccca ttgctggtgt tgtattagcc gcacgcccgg tcataaacca agaagactgg
     2761 acacaaaaaa accgcatgtc tgtcgggcgc ggtaaccgct acttgctaag gtgttttgag
     2821 caccatgagg cggaatatat ccccgttaat gcagcattgt caaacgatct gttctttaaa
     2881 gacttaacga gaagcctttt cgggaatttc gatctgacaa tcttgaaata tcctgtagag
     2941 tacggcgcat ttcttccgtc agatgcttga aggcgttaaa ttcggcgaca gctgcatcga
     3001 cgttatagcc cttgctgtgt agctcgttga ggatgcgcat agttgggctt ggcatatcga
     3061 agagcacaga aacgtccagg ttaagagttt ttcgatcaac atagctcatc atggagtagg
     3121 ggtgatgctc tgaaaaccac gagagagggt agttgataga tagtgttgat tctggaagct
     3181 gaagttgttc tgggattttt tgactgaaat agcagtcttc cagtttttcg aacacttccc
     3241 acgcccgatc ggtttcgagc atttttgcgt gacgggctgc gccgcgttct gtccagagga
     3301 tgagggagcg ggctttcggg gaaatttgta accctcttaa agatggttgc aaattttgtg
     3361 agttacttaa agtaacccgc aaattttgtg agttacttaa agtaacccgc aaattttgtg
     3421 agtagtttaa agctacccgc aattctttaa ggtcattacc aacaactttg aaaaagtgtt
     3481 tcccttcaac gaagcgtact ttgttctcat gatgattctg gcgaatacgc accggctcag
     3541 tgccgtaaag ctgcgccaaa agttcggtgg taataacagg aatctggtta tgggtgatcg
     3601 gggagagagt ttcaacagaa attttagttg tcataatgac gccctctggt ggtttcttaa
     3661 taactcacca ccgacgacgc caatcgtctg gtggtgaact gtgcagggtt ggcgtaaccg
     3721 ggaaaccgac cggcgcggat ctctccgccc ccacacagcc caccataatt cagatgtgcg
     3781 cgtgcatacg acaataaaaa acacgctcgc ggcgtgtatc tgtcgcggtc tctatccagg
     3841 acgccaatcc cgacgccaga ttttgctggc gtttgaggaa tatagccccg aataaatcat
     3901 cacgtcaatc accttgtttt cctcgcacga tgtcttagcc accggatatc ccacaggtga
     3961 gccgtgtaat tgaaggtttt tacgtcagat tcttttggga ttggcttgcg tttatttctg
     4021 gagcgtttcg ttggaaggta tttgcagttt tcgcagatga tgtcggtgat acttcgtcgc
     4081 tgtcgcctca tgcagccctc ctgacgccca gcccgatcgc catcaatgcc gctttggata
     4141 cggtagtaaa catccgtcga ggactgatga acggtcgcca aatcagcaac attgagcctt
     4201 tgctgtttcc cttcttctcc agccctgtcg atggttcgat aaaattaatc cgtccatcag
     4261 tgatgatgcg aacttcgtca acactctcca gagccttgct gaaccatccg acagacatat
     4321 cctctggcac aagcatcact accgtctgtc gctgttgtat gcactgctca gcggcttttt
     4381 ccacccacgg cctgatattg ctgtacggtg ggttattcca gattgcaccg tggcttatcc
     4441 actcagaatt tagcgcgtcg tcagcctcag ttagccagtg agcgcacaga gcgtttttgt
     4501 cgctcgcagc tgaatccagc cagaatccaa actcaatatc cagcgcatca aaaagccaga
     4561 gcggcgtttg ccagcagtcc ttgtcgtgtg ctggcgtatt tgatttgata gtcatgcagc
     4621 cttccctttt cgttgtgacc attcatactc tcgccgggag tcatcactcc accgcacgtt
     4681 gcgctctgag ccgaaccaga acatgatttc gataagctca gtcatgctgg cctttcgcat
     4741 tttgctggta cgcacgccaa gcatgacaac gccaccgtcg ataccaggcg cacttctttg
     4801 ctccagtttt ttggtcttaa gccacagggc agtgaacagg tctttccagt cttccggcgc
     4861 cagccgttga ccatgccata gcacctgacg cgaaacatcg ttcagcatcg gccacatgcg
     4921 gtcattctgc gctttgctgc gcttgggttc tttaacgtgg acttcgtggg gtgacttgtc
     4981 gtcgatgggt agtgagagaa tggcgtctat ggcgttattt ctgattgctt cgttgcgaag
     5041 cagaaaggtt tgcttcatct cctgctctcc ggttccattt ttcagtagcc gcagcaactg
     5101 atggtgccca tgccaccctg gcttcacaga ggtcacattc tgcatagccc cacacatcaa
     5161 tatttattcc ggcctcaacc cacagacgag cattaccgcc gcaaaacgga cattctttta
     5221 gctttggctg ggttaatgat aggtcgctca tgctcactcc ttcacttaaa atccagactc
     5281 cggataattc tgttgcgctg aaactcattg ttgagt
//
LOCUS       DBFIFS010000261         5219 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.261, whole genome shotgun sequence.
ACCESSION   DBFIFS010000261 DBFIFS010000000
VERSION     DBFIFS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.261"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(23..1399)
                     /locus_tag="N7A07_004309"
     CDS             complement(23..1399)
                     /locus_tag="N7A07_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU2478845.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRNEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(1396..2217)
                     /locus_tag="N7A07_004310"
     CDS             complement(1396..2217)
                     /locus_tag="N7A07_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2478846.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2204..2365)
                     /locus_tag="N7A07_004311"
     CDS             complement(2204..2365)
                     /locus_tag="N7A07_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478847.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(2398..2694)
                     /locus_tag="N7A07_004312"
     CDS             complement(2398..2694)
                     /locus_tag="N7A07_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU2478848.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(2836..3051)
                     /locus_tag="N7A07_004313"
     CDS             complement(2836..3051)
                     /locus_tag="N7A07_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2478849.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            3127..3822
                     /locus_tag="N7A07_004314"
     CDS             3127..3822
                     /locus_tag="N7A07_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2478850.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4324..4845
                     /locus_tag="N7A07_004315"
     CDS             4324..4845
                     /locus_tag="N7A07_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478851.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            complement(5023..>5219)
                     /locus_tag="N7A07_004316"
     CDS             complement(5023..>5219)
                     /locus_tag="N7A07_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478852.1"
                     /translation="SNKYNWLCVFLGERKRKPGMEAGLMISGVALSDNAHTHVQDFHA
                     LSVHRHSHELLDRIGHSNCM"
BASE COUNT         1337 a         1256 c         1203 g         1423 t
ORIGIN      
        1 attaactcag tgatgtagat ggtcatacgt cagccccttg tgcatatcgt ctgccacgcg
       61 cagcaggtgc atttgatgct gtgcaaatct gtctggcttc atcctggtca catgcaacaa
      121 agtgtccgtt acagaaccgc tggtaaactg taccaagcga gccaaaacgg tttttcgtca
      181 cgatgatttc agcaaatggc gcggcgctac tgttctcatc atataccgct tcccgataga
      241 gcatgatgat tgagtctgcg tcctgttcaa tgcttcctga atcacgcaaa tctgcgtttg
      301 tcgggcgttt gtttggtcgc ttctcaacat cgcgcgaaag ctgactcagg gagataaccg
      361 gtgttttcag gtctttcgcc atcgccttca ggcttccgga gatgtgagca attgcgaggt
      421 cgttgcggtc tgctttcggc ttctcaatca ggccaagata atccgccatg atgagtgaca
      481 ggtttggatt ttcctgctta tgccgttctg cgattgagcg tatttcttcg accgataacc
      541 gcgaagcatc gactacccat acatccaaat ctgcaagctg actcatgccg ttagcaacac
      601 gtgcccagcc ctcgtcatcc atcgatgcag gatttcgcag tacgctaacc gacatcctcc
      661 cggcgttggc aatgcttcgc tctgcaatct gcaatgcgct catttccatt gagaaaatca
      721 ataccccgcg ccggacgtca gaaccaggaa taacgcggct tgccacacct tcggcaatct
      781 tcagcgccag ttcggttttc cccataccag gacgagcggc gataatcacc aggtcttctg
      841 cgttcatccc tccagtgata gcgtcaagct cttcgattcc ggtcttcagg gtatctgact
      901 cttctccgtt cctcagacgc ctgtcaagcg tgtcagtgta gtcagtgatg atttccccta
      961 accgtacagg tttaacctcg ttacggggct ttctgatggc tgaaagacgc tttacaagtt
     1021 catccatcgc ctgactcgat gcgtcgatgg ttccgctttg gattggttca cgcatttcat
     1081 ccatgatttc cagcaccaga cggcggtgat agttatccgc gaccattccg gcatatccct
     1141 tcaggtttgc ggcactcggg cagtttttgc tggtcatcag gattgacgtg aaatgctcct
     1201 ctccgcacgc ctcggcaacc atcagcgcat cgattaggtt tctgtttctc gcctgcttgc
     1261 gaataacctc gaaggctttc cggtagagcg gaattgaaaa cgcttccggc tccagcgttg
     1321 ccagaacgtc actggcggtt ggagttaatc caccaatcag caggccaccg ataacgctag
     1381 cttcgatatc ctgtctcatg caatccccct gtctgcaaac ttcccttccc gaactcccgt
     1441 taacgaatct tccctcagca ggtaatcaaa atctgccgtc cagcccgtgt cgttgtctcc
     1501 gaagtaaaac ggcttggcct gatgcacaaa cgccctgaca tacgctctga aaccgtccac
     1561 gtttggcgtt ttcagttgcg ggataatttt cttcaggcgg cgttttcgtt tctcgttgac
     1621 cgcaacagcg tgtggaagtc tgtcaccgac ttcggtgttg taggcgttca ggaaggattc
     1681 gtagtcgatt cgttctgcct tgcgacgttc aggtttaacc tgcccatcgc catccccgtt
     1741 agggggtaag ggggtatttg tatttattgt cttttgtata ttgtcttttg tgtttagctg
     1801 acttggctta tacccattag ccgacttggc taatgtttta ttagctgttt tagctaatgt
     1861 taagctgtcc tggctaatcc actgagaaac caccttgttc actccgattt tcacgccatc
     1921 agcaatgagg aatttacgct caataagctg gcgcttggca gcgcaaacat gagtgtgatg
     1981 aatacctgtc atggctgcta tctgcgtgtt tgtgagtcga tccatcggct tattgaatcc
     2041 gtatgtcttg cgcatgatag cgagcatcac cttcaactgc cggacggtta aatcagccat
     2101 cagcagactg tcggtaatct cgttagcaac gcgcatgaaa ccatcttcgg tatctgccac
     2161 gcgatgctcc acgacctcca gttgaggcct gtaatcagct aacttaacga cgcccatgtt
     2221 tcactcctgc tttggctagt ctgtaaacac caacaaggcg ctctgcgaac gccctgttat
     2281 ttgctgcggc taccactaat ccctcaggtg aatcagggtg tcgaatctct tctttttcct
     2341 ggtatttctt acgacgtttt gtcataatta ctcctgtgga ttgatccagt aattccctca
     2401 gaattgcata tcaatttgct taaaatcctc ggtggcagcc ggggattttt tctttgtgat
     2461 ttcatcaagc gcatacttaa aagccctgct aatcggactg atgtctgatg ccattccgaa
     2521 agcacacaag accgaagcaa taaaccgcca gtccgttctg cttatcttcg attcatgaca
     2581 gccaatcatc tttgccagac cgcgctgtgt aagcgttgac aggttgatga gtaaatctgt
     2641 ttctgcgcga tcaacgtcgc gctgtgatag tttgctgtaa cttgttcgtt ccatttctta
     2701 agatttccaa tagtgaatag ttagttgaaa ggtatgcgtg gaaacgcata tggccttagt
     2761 tggtcagata tcttgggact cgcttttcag cgacgtagga cgaatgtccg ttgttacaaa
     2821 gagcggctcc gcttattaag cggctttgtg ctccggcggg aacacgtcat caagacttac
     2881 ttttgcgcct aacttgttta gacacgcaac aagagcacgg catgttttaa ggtctgggaa
     2941 gcgacgacca gattcccaat gtccgatagc tccctgtgtg catccaactg ccttagcaag
     3001 tgttgtttga gagatattca gtgactctcg atattttcgt aggttgctca tatgccctcc
     3061 atagtaacca cgaataaaaa aatacaatat gtacttctca aatacaagta aaaatacaca
     3121 ttgtgcatgg atggttccag tacagagcgt aataataagg acatgaaaat gaaatggtat
     3181 gaactggcta gatccagaat gaaagagctc ggcataactc aagagaagtt agccgaagag
     3241 ctaggtatga cgcagggtgg aattggacac tggttgcgcg gatctcgtca tccatctctt
     3301 agtgatattg gtgtggtgtt taaatacctt ggtattgata acatatcatt caaccacgac
     3361 gggacatttt cacctgttgg cgaatactca tcggccccag ttaaaaaaca atatgagtac
     3421 cctgtttttt ctcatgttca ggctgggatg ttctctccag aactcagaac ctttaccaaa
     3481 ggtgatgcgg agagattggt aagcacaacc aaaaaagcca gtgactctgc attctggctt
     3541 gaggttgaag gtaactcaat gaccgcacca acaggttcca aacctagttt tcctgacggg
     3601 atgttaattc tggttgaccc tgagcaagct gttgagcccg gcgatttctg catagccaga
     3661 cttggtggtg atgaatttac cttcaagaaa ctgatcaggg atagcggtca ggtgtttcta
     3721 caaccactaa acccgcaata tccaatgatc ccatgcaatg agagttgttc cgttgtgggg
     3781 aaagttatcg ccagccaatg gcctgaagag acgtttgggt aaagaggata gatggagttt
     3841 aatggccatg agtatcaagc ggtcaaaaag aagttcatct attcattgaa agcataaggc
     3901 catctgagta tatccgtaat gatctggata ttgtctataa catcaatgac cggacgatag
     3961 atatcggtga gcagcgtcct gtatggcagg gtgaggcagg tgaaaaaacg tcctgccatc
     4021 aacaagaatc aagtgcatcc gctctctgga cagatggaaa atttattgga tgcaaaaaga
     4081 tatgaaataa cacctgtata gcacggagct ttctctgacc gatgcacttg aagttgttag
     4141 ggctgaccca gcctgctgtt tctttaggta agagaaaaga gatttaggag atgaaaggtc
     4201 gcagaggtgc ggcctttttt attgagagtg gatcttgaac ggaaattctc aagacttgag
     4261 tcttgcatgc aaatcaattc ctggataaac tcgatctgag tcaataactt agagagagag
     4321 caaatggcaa agtcaaacgt tagcgtgcag gcattcaagg acttccttga agagcttatg
     4381 tcgctgaaca taatgaagga ggccaccgct cgaaatttaa aaaactcatc cgctcgcctc
     4441 ttaacggtag tccaagaaga ggaaatgggt gatgttactc agcttgatgt gaatgagctt
     4501 gccgagcgat acatcaacgc aactgagccg aagcctagcg acagcagcat tactgcatat
     4561 aaaagccgca tggaaagtgc aatcaaaaag tttgtagctt tccagtctgg tgaagaaatc
     4621 ccatacactc cgattgacaa agaatccagt gaggaaaaag atttgactgg cgaaccaaca
     4681 aaagtcgaag gcaaggctaa tgcacttcat acctatgatc ttccagtagt tcttcgacct
     4741 gaatcagggg ttacagtaac gattaaaggc attcctaacg atatcacaaa cgaagaagcc
     4801 gaacgcatct cttcaattct gaaggtttac gttcggcctc aataatgcaa aagcattcag
     4861 cacaatgtcc aactccccag tccgacattg atgctgttta accagagcct ccaaaaggag
     4921 cccttgttaa ggtacacaat atttgcgatg taaccttagc gcgtctggta cattttttca
     4981 agcggttgta gggctgccgc caatatgaga aaacacagat gtctacatac aatttgaatg
     5041 accaattcga tcgagaagtt catgtgaatg cctatgaacg gataaggcat ggaaatcttg
     5101 aacatgtgtg tgagcattat cgctcaaggc cacaccgcta atcatcaacc cggcctccat
     5161 gccgggtttt cttttcctct cgcccaaaaa aacacataac caattgtatt tattggaaa
//
LOCUS       DBFIFS010000262         5175 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.262, whole genome shotgun sequence.
ACCESSION   DBFIFS010000262 DBFIFS010000000
VERSION     DBFIFS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.262"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..139)
                     /locus_tag="N7A07_004317"
     CDS             complement(<1..139)
                     /locus_tag="N7A07_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478853.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RD"
     gene            complement(272..550)
                     /locus_tag="N7A07_004318"
     CDS             complement(272..550)
                     /locus_tag="N7A07_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478854.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            complement(621..911)
                     /locus_tag="N7A07_004319"
     CDS             complement(621..911)
                     /locus_tag="N7A07_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU2478855.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(908..1609)
                     /locus_tag="N7A07_004320"
     CDS             complement(908..1609)
                     /locus_tag="N7A07_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2478856.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1606..2544)
                     /locus_tag="N7A07_004321"
     CDS             complement(1606..2544)
                     /locus_tag="N7A07_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2478857.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(2577..2873)
                     /locus_tag="N7A07_004322"
     CDS             complement(2577..2873)
                     /locus_tag="N7A07_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU2478858.1"
                     /translation="MEQTNYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITNKKRPAATERSEQIQMEF"
     gene            complement(3012..3239)
                     /locus_tag="N7A07_004323"
     CDS             complement(3012..3239)
                     /locus_tag="N7A07_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478859.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            3318..4025
                     /locus_tag="N7A07_004324"
     CDS             3318..4025
                     /locus_tag="N7A07_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU2478860.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            4086..4427
                     /locus_tag="N7A07_004325"
     CDS             4086..4427
                     /locus_tag="N7A07_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HGU2478861.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            4495..4956
                     /locus_tag="N7A07_004326"
     CDS             4495..4956
                     /locus_tag="N7A07_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478862.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTAETNEAVLAALSAEVRGC"
     gene            4950..>5175
                     /locus_tag="N7A07_004327"
     CDS             4950..>5175
                     /locus_tag="N7A07_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGU2478863.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQY"
BASE COUNT         1323 a         1188 c         1170 g         1494 t
ORIGIN      
        1 ggtcacgcgg atagcgtcgt gcggcaggtg aatgctcata caactcatca agcggcaaac
       61 tggacgatga acgataccga taccaacgca ccaactcttc acgaaaatta gccctgacaa
      121 gctcagctat cgtactcatt tcttaaaacc tcctcaaacg cattctgacg catttttcat
      181 tctcgctgct tatcggtata ccttgcacgt gcttacctca ccacagagcg attgtgatgc
      241 cttaaaagcg atttattgaa gtgatatttg cttaatcgaa attcttttct ttgattcctg
      301 cggccctgat ggctttcatt actgcaatta ccgttttgtc acgcccatcc tcataaccca
      361 tcgcataagc accttcttca ccatctttcc aaaggtcgtc attcgattcg ggccagtcga
      421 tatccagttc aatagcagag cgcgatgcct gccatatcac ccaggcaaac tcttttaatt
      481 catcgtctcc cgtgaactgg cttttgtctt ttgaccacca gttttcaaac tgtcggtagc
      541 tatcgttcac ttccctctcc cccaaataaa aaggcctgcg attaccagca ggcctgtcat
      601 tagctcagtg atgtagatgg tcatctttta actccatata ccgccaatac ccgtttcatc
      661 gcggcactct ggcgacactc cttaaaaatc aggttcgtgc tcacctttcc ttcccgttct
      721 tccctggtag caaaccggta atacaccgtt cgccagacct taccttcgat aaccagaaga
      781 cctgcccgtg ccattttagc tgcggcctga tttatgctgg ttactgttgc gcctgttagc
      841 gcggcaacgt ccggcgcaca gaagctatta tgcgtcccca ggtaatgaat aattgcctct
      901 ttgcccgtca tacacttgct cctttcagtc cgaacttagc tttaatttct gcgatcttcg
      961 ccagcgcctg aacacgattt agaggtctgc cgcccatgac aggaagttgt tttactggtt
     1021 caggtatcgt ctcaccacgg ttaattcgcg ctgtcataca ggtcagttca tcggcagcct
     1081 tgcgtcgtaa ttccgcgtca gtcagcgcat tggcccgcat gttctggtac aggttggtaa
     1141 ccagccagta gtgcgcgttc gatttccacg gataagactc cgcatccgga tacaggcctc
     1201 gcttccggca atactcgtaa accatatcaa ccagctcgct gacgtttggc agtccggcga
     1261 taacggatgc ttcttcccgg caccatgcaa caaactgccc gggtgatggc agaaatggtc
     1321 gattctgccg acgggctacg cgcattcctg cgttaacctg ttccattgtg gtgatcccgt
     1381 tttcccggaa agccagaacc cactggcggc ggatttcatt cagttcgttc tggtcccggt
     1441 tagccagact cgccgggaaa gttgccagta actggctgaa cacaccgttg atgatctgcg
     1501 ctacctgttg tacctgcggc ttttcgtcgt actgttccgg catgttgttg gcgattcgac
     1561 gcatctgctc acggtcaaag ttaaccatct gtgcggcgat gtttttcata aatccacccc
     1621 gtaaatccag tcagtgtttg tcaggtcgag ttttggtttg ctggctgtca cgcctgcctg
     1681 ttgcttgtta cggttgattt cgagctgggt ccacttgtcg cggagtttgg ccggactcag
     1741 cacgttaccg gaccagaagt tgtcctggca ggcccagcgg aaaagcacac acatatcgcg
     1801 gtggttacgt ccgtcacgtt cacgcatcag gcggatatcg ttagcccacc cagcaaaatt
     1861 cggttttctg gctgatggcg cgatagtctt caccatgtca aacatccact ctgcggcggt
     1921 caggtcttct gctgttcccc acttgctgcc gctctgaatt gcagcatccg gtttaaccac
     1981 agaaagatcg ttttctggct ggtcagagga ttcgccagaa ttctcggacg aataatcttt
     2041 tcttttttct tttgtaatag tgtcttttgt gtccccctgt tttgagggat agcaatcccc
     2101 taatttgagg gatgttttat ccctcgtttt aggggatttt ccctcgtttt gagggatgtc
     2161 cctcatttta ggggaacctc cctcgttttg agggatgcac cattctgaga tgtttttatt
     2221 tggtccaaac atgccgcctt gctgcttgat aatattcatt ctgacgagtt ctaacttggc
     2281 ttcattgcac cgtttgacag gtaactttgt aatctcgcta agttgagaat cggtgattct
     2341 gtccattggt ttattccacc cataggtttt acgcagaatg gcaagcagca ctttaaactg
     2401 tcgcttggtc agatctgcgc ctgaataagc ctcaatcagc atatttgata gtctggcgta
     2461 accatcatcg agatctgcca cattacgctc ctgttcggca aagttacctc tgccgaagtt
     2521 gagtattttt gctgtatttg tcataatgac tcctgtagat tgatccagta atgacctcag
     2581 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgatt
     2641 tcatcaagcg catacttaaa agctctgcta atcggactga tgtctgatgc catgccaaaa
     2701 gcacacaaga ccgaagctat aaacctccag tctgttctgc ttatcttcga ttcatgacag
     2761 ccaatcatct ttgccagacc gcgctgagta agcgttgaca ggttgataag taaatctgtt
     2821 tctgcgcgat caacgtcgcg ctgtgatagt ttgctgtaat ttgtttgttc catttcttaa
     2881 gatttccaat agtgaatagt tagttgaaag gtatgcgtgg aaacgcatat ggccttagtt
     2941 ggtcagatat attgggactc gctttgtcag cgacgtagga cgaatgtcca ttgtgaaaat
     3001 agcggtgtta cttatgcagc cagaaggttc tttttgctta tttcaagcat ttcgcttgct
     3061 tgatatttgc caccagaaat ctcttcgatt tttgatgcgt atttagtttt cccaaaaaac
     3121 tcagtcttag ggaggaagcc gtttttgagc cacttataga cagccctttc gctaactcca
     3181 caagccttcg caacttcagg gatgccgaca cctttaatcg gctcatcaag attttgcata
     3241 ggaatatcct ttttcgtact ttcagtacgt attatggttg aactgaaagt ttttgcaagt
     3301 gctttagtat cgtactcatg gttcagaatg aaaaagtgcg caaagaattc gcccagcggc
     3361 tagcgcaagc ctgtaaagaa gctggtcttg atgaacatgg taggggaatg gctatagccc
     3421 gtgccctttc tctttcgtcc aaaggcgtta gcaaatggtt taatgctgag tctttaccgc
     3481 gtcaggaaaa aatgaatgcg cttgcgaaat ttctaaacgt tgatgttgtt tggcttcagc
     3541 acggcacttc gttaaatgga gcgaatgatg aagatactct ttcatttgtt ggcaaattaa
     3601 aaaaagggtt agtgcgcgtg gttggtgagg caattcttgg tgttgatggt gccatcgaga
     3661 tgaccgaaga gcgcgatggg tggctcaaaa tttatagcga tgatccagat gcctttggtc
     3721 ttcgtgtgaa aggagacagc atgtggccca gaataaaatc aggagaatat gtactcattg
     3781 agcctaacac caaagtattc ccgggtgatg aggtgtttgt cagaaccgtt gaaggacaca
     3841 acatgattaa ggttcttggc tatgacagag atggagaata ccaatttaca agcattaacc
     3901 aggatcacag gcctataacg ttgccttatc atcaagtagc aaaggtggag tatgtagctg
     3961 gtattctgaa gcaatctcgc catctggatg acatcgaggc aagggagtgg ctgaaaagtt
     4021 cgtgacttca tcgtcacata gctggtaacc agtggcctga agagacgttt gggtaaggag
     4081 gatagatggc gttcaatgac cttgaatatc aagcagtaaa aaaagaagtt caccaattca
     4141 ttgaaagcat aaggccgcct gaacatatcc gcaatgaact ggatattgtt tatagcatca
     4201 atgaccaaac gatagatatc ggcgaacagc gccccgtgtg gcagggcaat ccaggtgaaa
     4261 caaacatcct gccatcagca agaatcaagt acatacgttc tctggataga tggaaaatct
     4321 attggatgcg gaaggatatg aaatggcatc agtacagtac tgaactttcg ctgactgatg
     4381 cgcttgagct tgtgcgtgct gacccggatt gctgcttctt cggatgagtg aagagacgtt
     4441 tggatgatgg atggtcgcag agatgcggcc tgattctaaa ataggatata aaaaatgaga
     4501 atactaggtg ttagagcggc gcccaaagtt acatcttttg ttgtatattg cactaatgag
     4561 tctgcactca aatgtgttga tgttattaaa ataccttcga ccttagacac accagaaaaa
     4621 ttaaagtatg tgagaaataa catcctcgac attcttaatt tatataatgt tgaattagct
     4681 gccatacgcg ttactgaatc aaactctgat aatcttagca ttgaccgcct ttatatagaa
     4741 gctgttattc aagaagcatt ttcaagcagt gatgtaagaa aatattacac tattagaaaa
     4801 tctggcatga aatcatcatt gaacctaaca gagatcgagt ataaagaaat attgaagtca
     4861 caccgcaata taaatggaat cgataattct ggttttacag ctgaaacaaa tgaagctgtt
     4921 ttggctgcac tatctgcgga ggtaagggga tgctaactcc atacaaaaga gctgatgtag
     4981 aattcgaatg gattagtgat ctagaagaac agggttgttt ttcaaaagta tatctggctc
     5041 atgacagaca cctagctcat gacttggtga ttaaagaaat agaaaaaaaa gaaaacacta
     5101 accacgacga ctactttaat gaagcaaggc ttctctataa acatgcacat ccaaatattg
     5161 tgcaagttca gtatg
//
LOCUS       DBFIFS010000263         5148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.263, whole genome shotgun sequence.
ACCESSION   DBFIFS010000263 DBFIFS010000000
VERSION     DBFIFS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.263"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..379)
                     /locus_tag="N7A07_004328"
     CDS             complement(<1..379)
                     /locus_tag="N7A07_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478864.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="N7A07_004329"
     CDS             complement(436..831)
                     /locus_tag="N7A07_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2478865.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="N7A07_004330"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="N7A07_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="N7A07_004331"
     CDS             complement(1642..2382)
                     /locus_tag="N7A07_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2478866.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="N7A07_004332"
     CDS             complement(2389..3351)
                     /locus_tag="N7A07_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2478867.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="N7A07_004333"
     CDS             complement(3374..3799)
                     /locus_tag="N7A07_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2478868.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="N7A07_004334"
     CDS             complement(3796..4098)
                     /locus_tag="N7A07_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2478869.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="N7A07_004335"
     CDS             4196..4567
                     /locus_tag="N7A07_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478870.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="N7A07_004336"
     CDS             4588..4779
                     /locus_tag="N7A07_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGU2478871.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="N7A07_004337"
     CDS             4781..5059
                     /locus_tag="N7A07_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2478872.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT         1230 a         1431 c         1141 g         1346 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgaagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gtttagtttt tacttgac
//
LOCUS       DBFIFS010000264         5148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.264, whole genome shotgun sequence.
ACCESSION   DBFIFS010000264 DBFIFS010000000
VERSION     DBFIFS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.264"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            101..1453
                     /gene="celF"
                     /locus_tag="N7A07_004338"
                     /gene_synonym="chbF"
     CDS             101..1453
                     /gene="celF"
                     /locus_tag="N7A07_004338"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU2478873.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNGVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1466..2224
                     /gene="chbG"
                     /locus_tag="N7A07_004339"
     CDS             1466..2224
                     /gene="chbG"
                     /locus_tag="N7A07_004339"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU2478874.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2271..4532)
                     /gene="katE"
                     /locus_tag="N7A07_004340"
     CDS             complement(2271..4532)
                     /gene="katE"
                     /locus_tag="N7A07_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU2478875.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            4715..4978
                     /gene="cedA"
                     /locus_tag="N7A07_004341"
     CDS             4715..4978
                     /gene="cedA"
                     /locus_tag="N7A07_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU2478876.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1254 a         1170 c         1358 g         1366 t
ORIGIN      
        1 ttatacctga caattcacat atctgtcctg ttgctggatt atttatgtcc gggggggcag
       61 atatgccaga tatcagtatt ctgtactgaa gggagaaatt atgagccaga aattaaaagt
      121 cgtcactatt ggtggcggga gcagctatac cccggagtta ctggaaggat ttattaagcg
      181 ttatcacgaa ttgccggtca gcgaattatg gctggtggat gtcgaaggtg gcaaagctaa
      241 actggatatt attttcgatc tctgccaacg gatgattgat aacgctggcg tcccgatgaa
      301 gctttataaa acgctggatc gccgcgaagc attgaaagat gctgatttcg ttactaccca
      361 actgcgcgtt ggccaattac cggcgcgtga actggatgaa cgtattccat taagtcatgg
      421 ttatcttggt caggaaacca acggcgcggg cggtctgttt aaaggtctgc gtaccattcc
      481 ggtgattttt gacatcgtaa aagatgtcga agaactttgt ccgaatgcat gggtgattaa
      541 cttcactaac ccggcgggaa tggtcactga agccgtttat cgtcataccg gatttaaacg
      601 ctttatcggc gtgtgtaata ttccgatcgg catgaagatg tttattcgcg atgttctgat
      661 gctgaaagac agcgatgatt tatctatcga tctgttcggc ctcaaccata tggtgttcat
      721 taaggatgtg ctggtaaatg gcaagtcgcg ctttgccgaa ttgcttgatg gtgtggcatc
      781 cgggcagtta aaagcatctg gcgttaaaaa tattttcgat ctgccattta gcgaaggctt
      841 aattcgttct ctgaatctgt tgccgtgttc ttatttgctt tattacttca agcaaaaaga
      901 gatgctggct attgaaatgg gcgaatacta caaaggcggc gcacgagcgc aggtagtaca
      961 gaaagtcgag aaacaacttt ttgagctgta taaaaacccg gagttgaaag ttaagccgaa
     1021 agaactggaa cagcgcggtg gggcttatta ctctgatgca gcgtgcgaag tgatcaacgc
     1081 tatctacaac gataagcaag cagaacatta cgttaatatc ccgcatcatg ggcatattga
     1141 taatattccg gcagactggg cggtagaaat gacctgtacg ctggggcgcg atggcgcgac
     1201 gccacatccg cgcattacgc atttcgatga taaagtgatg gggctgattc acaccattaa
     1261 aggcttcgag attgctgcca gcaacgccgc acttagcgga gaatttaacg gtgtgttact
     1321 ggcgctaaac cttagtccgt tggtgcattc cgatcgcgat gctgagctgc tggcacgcga
     1381 gatgattctg gcgcacgaga aatggctgcc aaattttgcc gactgcatcg cagagcttaa
     1441 aaaagcacat taaccgaggc tgattatgga acgcttactg attgttaatg ccgatgattt
     1501 tggcttaagc aaaggtcaga actacggcat tatcgaggcc tgtcgcaatg ggattgtcac
     1561 atcgacgacg gcgctggtga atgggcaggc tattgaccat gcggtgcagt tgagtcgtga
     1621 tgaaccgagt ctggccatag ggatgaactt tgtccttact atgggcaagc cactgacagc
     1681 tatgccgggg ttaacccgcg atggtgtgct gggaaaatgg atctggcagt tggcagaaga
     1741 agatgcttta ccgctggaag aaattactca ggagcttgcc agtcagtatt tgcgtttcat
     1801 tgagctattt ggacgcaaac ctacgcatct tgatagccat catcatgtgc atatgttccc
     1861 gcagattttc cctattgttg ccaggtttgc ggctgaagag gggattgcgt tgcgcatcga
     1921 ccgtcagccg ctatctaacg ctggtgattt accggctaat ctgcgcagtt cgcacggatt
     1981 cagtagcgca ttttatggtg aagagattag cgaggcactg ttcctgcaag tgcttgatga
     2041 ttccagccat cgcggggaac gctcgctgga agtcatgtgc catccggcgt ttgtcgataa
     2101 tacgattcgc cagagtgctt actgtttccc acggttaacg gagctggaag tgctgacatc
     2161 agcgtcgttg aaatatgcaa ttgctgagcg tggttatcgg ttgggtagtt atcttgatgt
     2221 gtaagtaagg tggtaaggtc actccccagg ggacgtgacc ttatccatat ttacgccgga
     2281 attttgtcaa tcttaggaat gcgtgaccac acgcggtgtg ctgccatcag cgttaacagt
     2341 tcatccataa aactaccgtc ggcgctgtca gcttccacaa tcccttcttc accctggtca
     2401 gcgaccttga ttgttgcttt aaacttgcgc gcgtctcccg ccagcgcaat cggtttaagg
     2461 tgtttgtagg cttccatcag gtagtagttg gcatcgccgt tgtcagcgat atccgcgata
     2521 ttgccgcaag ggacaatgac cgcatcgacc gtcagcgaag gcgcaccggc aaaggtagcg
     2581 gctataggca acaccgtacc gtcatccgca gtcacttcac ccattcggga gtagagcagt
     2641 ttggcatgaa cgcctttggc cttcagcgcc ttgagaatgg ccagaaggtc tgccgatctc
     2701 acttcatcat taagtaaaat cgctaccacg cgacctttca catcaccgtc aggaatggcg
     2761 tacaaactta aggatggatc ctttttcaga ccgttgacgt ccggaggtgg ggtgatattc
     2821 agctggtcgt cagtcagttc gataccgaga tttttcgcca ccgcctgggc cagagtgaga
     2881 tcaatatgcg ccagctggtc aacaacgcgc tcacgaatat acggacgaac gactttgctt
     2941 aactcaaaac tgaaaccatc gacaatatgg cgctgctcga atggcgtctg acttagccag
     3001 aacagacgcg gatgggaata atattcgcca aaagatggac tgcgctcgcg aactttattt
     3061 ccttccacgc gttcctgata tgattcaaaa ccgccgcgtt tcggtcccgg cggtgtttcg
     3121 cgcggccagt tatcgttgat cgagttcggt tcgtaattcg ccgggttagt gtcgatcccc
     3181 atacgatgca tgccgtcacg ctggaaatta tggtaagggc aggttgggcg gttaatcgga
     3241 atctcatgga aattcggccc accaagacga ctgatttgtg tatcggtata ggagaacaaa
     3301 cgtccctgca acaggggatc gttggtgaag tccagaccag gaacaatatg acctggatgg
     3361 aatgctgctt gttcgttttc ggcaaagaag ttatccggat tgcgattgag caccattttg
     3421 ccgacacgct gaacgggcac cagttcttcc gggataagtt tagttggatc gagaagatcg
     3481 aagtcgaact tgaattcatc ttcttcagga atcaactgga agcccagttc gtattccgga
     3541 aaatcgcctg cttcaatggc ttcccacaac tcgcggcggt ggaagtccgg gtcacgtccg
     3601 gttagttttt gtgcttcatc ccaaacgagt gaggctttac ctgccagtgg tttccagtgg
     3661 aaacgtacaa acgttgcctt cccttcggca ttaatcaggc ggaaggtatg aataccaaag
     3721 ccttccatgg tgcggtaact gcgggggatg ccgcgatccg acatcgccca catcacgttg
     3781 tgcagagttt caggttgcag agaaacataa tcccagaaag tatcgtgggc gctttgccct
     3841 tgtggaattg cccagtgtgg ttctggtttt accgcatgaa caaaatcggg gaatttatgc
     3901 gcatcctgga taaagaagat tggcgtgtta ttgccaacga ggtcaaaaat accctcttca
     3961 gtatagaact tggtggcaaa gccacggata tcacgcacgg tatcggcaga gccagcacca
     4021 ccctgaacgg tagagaacct aacaaatact ggggtgattt tgttcggatc tgagaggaaa
     4081 tccgctttgg tgatatcgct taagctttta tatggctgga aataaccgtg agcggctgat
     4141 ccacgtgcat gaacaatacg ttccgggatg cgctcatggt caaagtgggt gattttctcg
     4201 cgcagaataa aatcttccag cagcgttgga ccacggctac cggcacgcag tgagttttga
     4261 tcgtcggcga tgcgcacgcc ctgattagtg gtcagcgcat aattttcact gcctttgcgt
     4321 acgtcttcca gagaattaag tttttcgtta cgcgtatcag gggctttcag gctccctgag
     4381 gcggtaggtt gtgcacctgg cggtgttggt tcagccgctg gacgatgaga gccgtcctca
     4441 ggtgccagtg agtccatccc cggtttcgct tcgctggaat cgtgtagtgg tgactggtgc
     4501 tgatgtgggt tcttttcgtt ttgttgcgac attgaactcg tctccttaat ttattactga
     4561 aagggccgct gttttggtca aaaaactctc taactataga ccaccagcca gaccccgctt
     4621 cggagactat tctggaaacg gtgttatgaa atacacgcaa agcgggacgt acaggggcta
     4681 aatcggctaa actacagttt ttctgatttt taatatgcgt ttagtgaagc cagtaatgaa
     4741 gaaaccgctc cgccagcaaa accgccagat tattagctat gtcccacgca cggaacccgc
     4801 gccgccagaa catgcgataa agatggattc gtttcgtgat gtctggatgc tgcgtggcaa
     4861 atatgttgcg tttgtactga tgggagagtc atttctgcgc tcaccggcgt ttactgtgcc
     4921 tgaatccgcc cagcgttggg caaatcagat ccgccaggaa ggggaagtga ctgagtaaag
     4981 attacgtacc tcccggtttt gccgggaggt acatggcctg aaattctcta tttcattgct
     5041 acattcattc aaaccacctg taacaaaaag ctgcctttga cgatctattg ctataaataa
     5101 gtgatctttt ttctttaatt gtttatttat tttaaaatgg ttaagttt
//
LOCUS       DBFIFS010000265         5064 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.265, whole genome shotgun sequence.
ACCESSION   DBFIFS010000265 DBFIFS010000000
VERSION     DBFIFS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.265"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1341
                     /gene="paoC"
                     /locus_tag="N7A07_004342"
     CDS             <1..1341
                     /gene="paoC"
                     /locus_tag="N7A07_004342"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU2478877.1"
                     /translation="QHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGA
                     NRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQID
                     PADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEK
                     SGARVHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSA
                     GSGGQWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATA
                     GGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPK
                     TARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDT
                     DPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1351..2307
                     /gene="paoD"
                     /locus_tag="N7A07_004343"
     CDS             1351..2307
                     /gene="paoD"
                     /locus_tag="N7A07_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGU2478878.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(2486..3613)
                     /locus_tag="N7A07_004344"
     CDS             complement(2486..3613)
                     /locus_tag="N7A07_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2478879.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3755..4813)
                     /locus_tag="N7A07_004345"
     CDS             complement(3755..4813)
                     /locus_tag="N7A07_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2478880.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
BASE COUNT         1175 a         1452 c         1409 g         1028 t
ORIGIN      
        1 cagcacttgc gtatcggtgc cgaccagagc gggaaaatca ccgctatctc acatgaaagc
       61 tggtctggaa acctgcccgg cggcacgccg gaaacggcgg tacagcaaag cgaattactc
      121 tacgccgggg caaaccgtca taccggcctg cggctcgcca cgcttgattt gccggaaggg
      181 aacgccatgc gtgcgcccgg cgaagccccc ggtctgatgg cgctcgaaat cgcgatcgac
      241 gaactggcgg aaaaagcggg catcgatccc gtcgagtttc gcatcctgaa tgacactcag
      301 attgaccccg ccgacccgac gcgccgcttc tctcgccgtc agcttatcga gtgcttgcgc
      361 accggagcgg ataaatttgg ttggaagcag cgcaacgcca cacccggaca ggtgcgcgac
      421 ggggagtggc tagtcggcca cggcgtcgcg gcgggctttc gcaataatct gctggaaaaa
      481 tcgggggctc gggttcacct cgaaccaaac ggcaccgtta ccgtggaaac ggacatgacc
      541 gacattggca ccggcagcta caccattctg gcccagacgg cagcggaaat gcttggcgta
      601 ccgctggagc aggttgcggt tcacctcggc gattccagtt tcccggtttc tgcgggttct
      661 ggtggacaat ggggcgcgaa tacctccacc tccggcgttt acgccgcctg tgtgaagctt
      721 cgcgaaatga ttgcctcggc agtcgggttt gatcctgagc agtcgcagtt tgccgacggc
      781 aagattacca acggtacccg aagcgccata ctacatgagg ccaccgcagg cggcagactg
      841 acagcggaag agagcattga attcggaaca ctgagcaagg agtaccagca gtcgaccttt
      901 gccgggcatt ttgtggaggt cggcgtgcat agcgcgacgg gagaagttcg ggtccggcgt
      961 atgctcgctg tgtgtgctgc aggacgcatc ctgaatccga aaactgcacg cagccaagtc
     1021 attggcgcaa tgactatggg catgggcgcg gcactgatgg aggagctggc ggtggatgac
     1081 cgtttgggct acttcgttaa tcacgatatg gcggggtatg aggtgccggt ccatgcggat
     1141 atcccaaaac aggaagtgat tttcctggat gataccgacc ccatatcctc cccgatgaag
     1201 gccaaaggtg tcggtgagct gggcctgtgc ggcgtgagcg cggctatcgc caacgcggtg
     1261 tataacgcca ccggtattcg ggtacgcgat tatcccatca ctctggataa gctgctcgat
     1321 aagctgccgg atgtggttta aggaggaaca atgtcatacc cgctttttga taaagacgaa
     1381 cactggcata agccagagca ggcgtttctc accgatgacc accggaccat tctgcgcttc
     1441 gccgtagagg cgctaatgtc cggtaaagga gcggtgctgg tgacgctggt ggagatacgc
     1501 ggcggcgcgg cgcgcccgct cggggcgcag atggtggtgc gcgaagatgg tcgttactgc
     1561 ggttttgtct ctggcggctg cgtggaggcc gctgccgctt ttgaagcgct ggagatgatg
     1621 ggctcaggct gcgatcgcga aattcgctat ggcgaaggtt cgccgtggtt tgacatcgtt
     1681 ctgccctgcg gcggtgggat cacgctgacg ctccataaac tacgctcggc acagcctctg
     1741 ctcgccgtgc tgaaccgcct ggaacagaga aaaccggcgg ggctgcgcta cgatccgcaa
     1801 gcacaatcgt tggtgtgcct gcccacgcaa acccgaacgg gctggaatct caatggcttt
     1861 gaggtggggt tcaggccatg cgtcaggctg atgatttacg gacgttctct tgaggcgcag
     1921 gcaaccgcga gtcttgcagc agccacaggc tatgacagcc atatcttcga tctttttccg
     1981 gcctcagcca gcgctcagat cgataccgat acggcggtca ttttgctgtg ccatgatctc
     2041 aaccgggagc tgccagtgtt gcaggccgcg cgagaagcaa aaccctttta tctcggcgca
     2101 ttgggcagct atcgaaccca cacgttacgt ctgcaaaagc tccacgagct gggatggtcc
     2161 agggaggaga cagcgcaaat ccgggcaccc gtcgggatat tccccaaagc ccggaatgcg
     2221 catacactgg ccctctccgt gctggcagaa gtcgcctctg tacgtctcca tcaggaggag
     2281 gattcatgcc tgcccccgtc gtcctgatcc ttgcggccgg gcgtggaaag cgctttctca
     2341 cctccggggg aaataccata aattaagaga aaaatttgca gtagccgttt tagaaaatca
     2401 cctaaatcgt ttaaaagagc gccctaccca tagtggttgc tcatcgcctt gtgtaacttt
     2461 ttcagcaacg cgcgccctcg ccggactaaa gcgcaccggc cttctggcgt gctgtaacaa
     2521 aggtcagtac gcccgcgcaa aggagcagca cactactcat ggcaaacgta ctctgccagc
     2581 caaggcggtc aaacacgatg cctcctgccg ttgatccaag ggcgattgag agctgaatca
     2641 ccgcgaccat gagcccgcct ccggcttcgg cgttgtcagg caacgtacgc gcaatccatg
     2701 tccaccatcc ggttggcgcg gcggtggcca gcatgcccca gagtcctaac agcagcgaga
     2761 ccatccagac gtgatgcccg gtaagcatca gaccgatggc aatgacggcc atcagtccgg
     2821 gtatggccat cagcgtgggg tagaattttc tgttgagaaa tatcgaaaca accagcgtgc
     2881 caataaaacc ggccacgccg ataatcagca aaatcaacga caagcctaag gaattaaccc
     2941 gtgtcaccga ctccagaaat gggcgcacat aggtgaatag cgcaaactgc cccatgaaaa
     3001 acaggccaca agctatcatg ccgaccacaa ccatgcggcg gctaaataag cggaataccg
     3061 cgccacatga cgtgtggctt ttattcgcat ccatgctggg cagactaatg cactgccaga
     3121 taaaggcgac aaccgccatg ggcaccaagc acaagaaagc gcctcgccat ccgaccgtag
     3181 cccccagata gcttcccagc ggcgcggcca cgaccgtcgc cagcgcatta ccggcgttga
     3241 aaatcgccag cgcacgcgtg acctgatgct gcggcaccag acgaatcgcc gttgccgctg
     3301 acatcgacca gaatccgcca atggccacac caatcatcgc acgaccgacc atatacatca
     3361 gataactggt agccagcgca ataataagcc ccgatacggc catcagaacc gtcatcccca
     3421 gcagcaggaa cttacgattc atctttccag ctagcgttga aagcgtcagg ctggtcagaa
     3481 ccgccagcgc accggagata gcaatccccc gccctgccag cccctccgtc acgcccaggt
     3541 cacgggcaat cggcgtgagc aggctgacgg gcataaattc agaagcaatc agcacaaaca
     3601 cgcacagggt catggcaaaa ataccgcccc agtatgcgtg ctgatgatga ggtgatggtg
     3661 tttggcttag tgtcgacata gtgaaatttc acaaagatca ggataaaagc caccggccat
     3721 agaccggtgg ctttgcatca attcagggga ggttttattt cagtttggtc tggaaaaatt
     3781 gttcgaactt cgcgaaaggt atttttccgg ccacgttatc gtacaggtcc acgtgatttg
     3841 cccctggtac caccaccaac tctttgtctt tactgccaac cgccttgtaa gcatcttcag
     3901 caaaatagcg tgaatgtgct ttctcaccgg tcacaatcag cgtcggaatg gtgatttcac
     3961 tggcgtagct caacagcggc atattcataa atgacagcgg catcgttgcc gtccacgccc
     4021 cggtagagtt aacggaacgt tcgtggaagc cgcgcggcat gcgatagtag tcaaagaact
     4081 ctttcagtac cggatgagga tttgccggca gcgtttctgg cagaatgcgc tcgcccgcac
     4141 tgaccttgcc gttgctatcg acattaatat catgaccacc gtgagcgaac gttccgctct
     4201 gcgcatcctt ccagcgctgt tcattcaaat attgcagaac agcacgacgg tcggcggtag
     4261 aataacggtc ttcgccatcc cccacaccat ggcccatcgc ccggctcatg tcatacatca
     4321 cgctggtagc aaccgcttta acgcgggtat ccatcgccgc gtcgttcagc gccatgccgc
     4381 cccagccaca aataccgagc agcccgatac ggttgcgatc tacctctttt tgcagcccta
     4441 agaaatccac cgccgcgctg aaatcttcag tgttgatatc cggtgaggcg acatttcgcg
     4501 gatagccgcc gctttccccg gtgtaagaag gatcgaacgc cagggtaaca aaccctcgtt
     4561 ccgccagcgt ctgcgcatac aagccgctgg attgctcttt caccgcgcca aatggcccac
     4621 tgaccgcaat cgccgccagc ttgcgatcgc cacggtcttt aggcaggtaa agatcgccca
     4681 ctaaggtgat gccatatcgg ttcgggaacg agactttacg gtgatcgact ttctggctct
     4741 cggcgaacgt tttatcccat ttatcggtca tggacacagg ggcattagga ttggttgaat
     4801 cagcataact cattgtcgtg actccactta atgatgcgaa aagcagcatg accggcatcg
     4861 cggtttttag cgttcgggtg aaggctttca taaaaatccc cataaaaaac aacagagaga
     4921 tacaggtttc cgacggacaa cgctgcggtg ctttactttg atggggtcat tatagtgagc
     4981 agaattagtg ctgattaggg ggcgattaat gctaagatta ataccatatt gttataaatt
     5041 aatgctgaga ttgccggcat gaag
//
LOCUS       DBFIFS010000266         5049 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.266, whole genome shotgun sequence.
ACCESSION   DBFIFS010000266 DBFIFS010000000
VERSION     DBFIFS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.266"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            84..1322
                     /gene="alaC"
                     /locus_tag="N7A07_004346"
     CDS             84..1322
                     /gene="alaC"
                     /locus_tag="N7A07_004346"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU2478881.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1387..1458)
                     /gene="ypdK"
                     /locus_tag="N7A07_004347"
     CDS             complement(1387..1458)
                     /gene="ypdK"
                     /locus_tag="N7A07_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU2478882.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1814..2734)
                     /gene="lpxP"
                     /locus_tag="N7A07_004348"
     CDS             complement(1814..2734)
                     /gene="lpxP"
                     /locus_tag="N7A07_004348"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU2478883.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3087..3329
                     /gene="yfdY"
                     /locus_tag="N7A07_004349"
     CDS             3087..3329
                     /gene="yfdY"
                     /locus_tag="N7A07_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU2478884.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3406..3681)
                     /gene="ypdI"
                     /locus_tag="N7A07_004350"
     CDS             complement(3406..3681)
                     /gene="ypdI"
                     /locus_tag="N7A07_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGU2478885.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            3977..4612
                     /gene="yfdX"
                     /locus_tag="N7A07_004351"
     CDS             3977..4612
                     /gene="yfdX"
                     /locus_tag="N7A07_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU2478886.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKKLTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         1369 a         1141 c         1222 g         1317 t
ORIGIN      
        1 ttaatcaggc tattttcaaa acagccacca tcgtggcagc gtcgctcgga cggtccgggc
       61 gctaacgtta atctgaggaa attatggctg acactcgccc tgaacgtcgc tttacgcgca
      121 ttgatcgtct cccgccctac gtttttaaca tcaccgctga actgaaaatg gctgcgcgtc
      181 ggcgcggcga agatattatc gatttcagca tgggtaaccc ggacggtgcg actccgccgc
      241 atatcgtcga aaaattatgt actgtggccc agcgtccgga cacgcatggt tactccactt
      301 cacgcggcat tccgcggtta cgtcgcgcca tttcccgctg gtatcaggat cgctacgacg
      361 ttgaaatcga tccggaatca gaagccatcg tcaccattgg ttcgaaagaa gggctggcac
      421 acctgatgct ggcgacgctg gatcatggtg acacggtgct ggtgccgaat ccaagctacc
      481 cgatccatat ttatggcgcg gtgattgccg gggcgcaggt acgctcagtg ccgctggtgg
      541 aaggtgtcga tttcttcaac gaactggagc gcgccattct tgaaagttat ccgaaaccga
      601 agatgatgat cctcggcttc ccgtcaaacc caaccgcgca atgcgtggaa ctggagttct
      661 tcgaaaaagt agtggcgctg gcgaaacgct acgatgtgct ggtggtccat gacctggcct
      721 atgccgatat cgtctacgat ggctggaaag cgccgtcaat catgcaggta ccgggtgcac
      781 gcgatgtggc agtcgagttc tttacgctgt cgaaaagcta caacatggcg ggctggcgta
      841 tcggctttat ggtcggcaac aaaacgctgg tcagtgccct ggcgcgaatc aaaagctatc
      901 acgattacgg cacttttacg ccgttgcagg tggcggcgat tgcggcgctg gagggcgatc
      961 aacagtgcgt gcgcgacatt gccgaacagt acaaacgccg ccgcgatgta ttggttaaag
     1021 ggctgcatga agcgggctgg atggtcgaaa tgccgaaggc ttcgatgtat gtctgggcga
     1081 aaatcccgga accatatgcg gccatgggat cgctggaatt tgccaagaag ctccttaacg
     1141 aagcgaaggt ctgtgtctct ccagggattg gctttggtga ctacggcgac acgcatgttc
     1201 gctttgcact gattgaaaac cgcgaccgta ttcgtcaggc gattcgtggg attaaagcga
     1261 tgttccgtgc cgacggttta ctacccgcca gcagcaaaca tattcacgaa aacgcggaat
     1321 aaacaccgtc gtcagaaatg caaacaggag cctgatggct cctgtttttc attgcacagt
     1381 ttgcttttag atcatcaggg caaaagtacc ggcccaaacg atgaccataa aagaaatgcc
     1441 cataaagaaa tatttcactt tacatctctc cgcgtaatta cggttacgcc tgaatgactt
     1501 catcacacct gatacagtga gttgacaccg ggcaaaacga ccttaaaagt cctgtatttt
     1561 accgtgggca tcaccccaga attctgagct aaactgctcg agacggcgct aatgtacgcc
     1621 taaaactgaa acgagacaag aggcattttc ttatttactt ttagtaactt acaggtgtct
     1681 catatcggcg acagtttaat ttctggcggt aataattaga tattcagcga aggaaaaaat
     1741 cttaagaaaa aagccataaa aaccatgagg ttattatggc cgatttgagg agggaaagag
     1801 taagagcagt ttgttaaatg tacaacgacg attctcccac cgggcgcgtt ttaaagcgac
     1861 ggtggatcca gaggtactgc tccggtgcgc gcatgatctc tttctcgata atcttgttca
     1921 tataggcagc ggcttgattt tcatctgtcg ggtagccttc catctctggg gtgatgaaca
     1981 aacgatatcc gctgtaatcc gcttttctta ccatcgttac ggtcaacatg gctgcgccag
     2041 agagacggga gagaacatag gtgccattgg ttgtggcgac attttccacc gcaaagaacg
     2101 gcgcgaagga gctgccttta cgaccataat cctgatcggg agcaaaccat accgcttcac
     2161 ctttcttcag tgcaccgaca atgccgcgca gattatttct gccgatcatc gctttgttag
     2221 agcgcatgcg cccacgggtc tgcacccatt ccatcagctg attattatgt ggacgatagg
     2281 tagccatcat tggctgacaa agtcccatca cgcggccgcc cagttccagc gacataaaat
     2341 ggacgccgac aaccattacg ccgcgatttt gcatttgtgc gcgtttaagg ttatccagcc
     2401 cttcaacatc aaaccattta cgtacgcgac tgtcgggcca gaaccacgcc atgccggttt
     2461 ctaccagagc catgccgagt gaacgaaagt tttcggcaat catcttctcg cgttcttccg
     2521 cagaatgctg cgggaagcaa agttccaggt ttttacgggc gatagattca cgccgtttca
     2581 ggaatggtcg cgccattgcg ccaatacgcg tgccgagaaa gcagagaaca ggataaggaa
     2641 gctgtaccca aagccagagt acaccaagcc caaaccatgt gagccagtag cgaggatgta
     2701 gaaactcgcg ggaaaatttg cattgtggaa acatagttac cttctgtaga tacgaattgt
     2761 tgtatcgcag aagattccat gtcgacagcg gtacactcaa ttggttgggc catcatgtac
     2821 gacaatggtt cctgtaaaga gtgtaaagac tagtcaggac ataaccacga ggagcatgaa
     2881 tgcggtatct gtactttatc actggctgac tgctttcagc tactgattat taatcgtgca
     2941 aagtaaaaaa ttttccgcac gcaatagtat tgcatttaac atgctgtttt cattgacgct
     3001 tagcaaaaat tgccagaggt taaggattgt cttactatta aagtgactga agttacaggt
     3061 tttttagcgc ggcaggaggt tgagctatgt tttatttatg gatgtttctc gccctgtgta
     3121 ttgtctgcgt aagcggatac atcggtcagg tactgaacgt ggtatcggcg gtctcttctt
     3181 tcttcggcat ggtgatcctc gctgcactca tttattactt caccatgtgg ttaactggcg
     3241 gtaatgaact ggtgacgggg atatttatgt ttcttgcccc ggcttgtggc ttgatgattc
     3301 gctttatggt ggggtatggc aggagatagt gggtcaggat ggttcatttg ggcaggtgtt
     3361 cacatcagca tcagacatga ttgttcacct gccaggtgac agacgctatc gcacaggatg
     3421 aataagataa ttcgcctttg tcggaaaagg gggcgtggtt tcttctgtga ggtttaatgg
     3481 tggtgcatct tctctcatct taaacaaaat cgcctgtgat ggctgaaatt ttgtagagtg
     3541 agttgtcgtc atcgctgacg ttttttctgg ctctttatag ctcgtctcac taccaccacc
     3601 agaattagaa aatgcaaaaa cattgcatgc cataatagag accaataaaa ataagctaaa
     3661 aagtattaag tttactttca tagaaagtta tcctgaaaat ggttcactga gaatgtacga
     3721 tgttatggct gtatttacat ataattaatc aatatttaca tcgatataat aaatgacatc
     3781 tctttgtggt acataagaat agttctctgt gacaggaagc atattcctac aattgtaaga
     3841 ctaaaatact tcttgcgata ataactacaa ctgtaagata accctttcaa aatgaccgtt
     3901 gctctctgat ttctcatttc atgctcaccc aatatgatgg cggcgttttc taaaactgtt
     3961 aaagaatgag gtaagtatga aacgtttaat tatggctacg atggtcacag ctattctggc
     4021 atcttcaact gtatgggctg ctgataatgc tccggtagcg gcacaacagc aaactcagca
     4081 agttcagcaa acccagaaaa cggcagctgc agcagagcgt atctctgaac aaggtttata
     4141 tgcgatgcgt gacgtgcagg tagcgcgcct ggctctgttt catggcgatc cggaaaaggc
     4201 aaaaaaactg accaatgaag cttcagcttt gttgtcagat gacagtaccg aatgggcaaa
     4261 gttcgctaag ccaggtaaaa agaccaatgt caatgatgac caatatattg tcattaatgc
     4321 atctgtcgga atttctgaga gttatgtagc gacacccgaa aaagaagccg cgataaaaat
     4381 tgccaatgaa aaaatggcga aaggggataa aaagggcgca atggaagagc ttcgcctggc
     4441 cggtgttggt gtaatggaaa accaatatct gatgccgctt aaacagactc gtaatgcact
     4501 tgctgatgcg cagaaactgc ttgataaaaa gcaatactac gaggcaaacc tggcgcttaa
     4561 aggtgcggaa gatggaatca tcgtcgatag cgaagcactg tttgtgaact aatgctgaca
     4621 ggcagtatat tgaggtaaag gatttaactc cctccagccg acaatttttt atgaccacaa
     4681 tacgacaggt tgtggtcata aaattatttg gcgctcgctg ttctataaac tcgcatttta
     4741 tattcctcaa agtaaaccgc aataatcccg tgtcatcaca aatcatttaa tatgttatat
     4801 tttatggtac gctctaaaaa gcgtaatcat acccttactt gtaaatcacc ggcgcttctt
     4861 acagttgtaa gaataacatc acaaatgtcg cgactctctc tgattaccac gaaccaatat
     4921 ttatcatatt cttcttttcg tcctcactac ttaccatgca gaaaggaatt ggaagtagcg
     4981 atgaaatgat tttcagcact tgatacagga atttaaaaat atcgagtgcc gctatgacga
     5041 tattttgcg
//
LOCUS       DBFIFS010000267         5023 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.267, whole genome shotgun sequence.
ACCESSION   DBFIFS010000267 DBFIFS010000000
VERSION     DBFIFS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.267"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(456..1031)
                     /locus_tag="N7A07_004352"
     CDS             complement(456..1031)
                     /locus_tag="N7A07_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2478887.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(1100..1678)
                     /gene="terD"
                     /locus_tag="N7A07_004353"
     CDS             complement(1100..1678)
                     /gene="terD"
                     /locus_tag="N7A07_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU2478888.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1727..2767)
                     /gene="terC"
                     /locus_tag="N7A07_004354"
     CDS             complement(1727..2767)
                     /gene="terC"
                     /locus_tag="N7A07_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU2478889.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2790..3245)
                     /locus_tag="N7A07_004355"
     CDS             complement(2790..3245)
                     /locus_tag="N7A07_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU2478890.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(3268..4425)
                     /locus_tag="N7A07_004356"
     CDS             complement(3268..4425)
                     /locus_tag="N7A07_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2478891.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4425..5006)
                     /locus_tag="N7A07_004357"
     CDS             complement(4425..5006)
                     /locus_tag="N7A07_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2478892.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1254 a         1351 c         1205 g         1213 t
ORIGIN      
        1 aaggggtatg ttttgtccgg attgtaaatt catattaact ctcctgattt ttgattatta
       61 ttaatgcgca gcgtttatat atgttcccta ggcttagttc tggacgctgg atattcggtg
      121 aggcgtaaat atggtatgac accatttttc ataacgctga agtttctata cctgttgaat
      181 ttgaattttc attgaccggg tatcttattt tccagggccc gttccttcat aagtcgcaaa
      241 agtaacatat atccgaaggg catgctgttg atatcagaca cggaatactg gctttaacca
      301 gcaaccacag ataaacccgg ggcctgcata aaaggttcat gccagaatta acatagctct
      361 gctttctgcc cccccccttt cccatattca cccgttgata gcggatcgat acccaaaaaa
      421 aaccccggca ttgccggggt cagactcagc gtcttttaga cattgacgcc gtgctgggaa
      481 gcaagcgctg acagaccacc ggcaaaaccc tggccaacag ctttaaactt ccactcagta
      541 ccatggcgat acagttcacc gaagaccatt gcggtttcgg ttgaggcgtc ttcagacaga
      601 tcgaaacggg caatttccgt cccgttgtcg ttgttgtaaa cgcgcatgaa gctgttgctc
      661 accatgccga agttttgttt acgcgcttct gcatcataga tggtaacggc aaataccagt
      721 tttttgatgt ctgctgagac tttggtcaga tcgattttga cctgctcatc gtcgccgtcg
      781 ccttcaccgg tacggttgtc gccctggtgc tctactgcgc catcagggct ggttttatta
      841 ttgaagaaaa tgaaatgggc atctgacagt actttaccgt cttcacctac tgcgaatacg
      901 gaagcgtcca gatcaaaacc ctgaccatcg gttacacggg catcccagcc caggccaacc
      961 atagcgacat tcatggttgg tgcttctttg gtcagagata cgttgccgcc ttttacgaga
     1021 gaaactgcca tttttagctc ctgcaaacag ttgaatgagg ctgaattaac agccccagat
     1081 atgaaaagtt acttttcgat caggacgcgt taatgccgta ctgagcacat acagatgcca
     1141 gaccaccagc ataaccctgg cctactgcgc ggaatttcca ctcaccattg tggcgataca
     1201 gctcgccgaa cagcatggca gtctcagtgg acgcatcttc ggtcagatcg tagcgagcga
     1261 cttcagtctg gttatcgtca ttaaccagac gaataaacgc accggatacc tgaccaaagc
     1321 tctggcgacg agcctgagca tcgtggatgg tcacaacgaa gatgatcttg tcaacttcag
     1381 acgggacggc gtccagttta attttcagcg attcatcatc accatcgccc tcaccggtgc
     1441 ggttatcgcc ggtgtgcgtt acggaaccgt cggatgacgt caggttgtta tagaagatga
     1501 aatctgaatc gccgcgcact ttgccgtttg aggccagcag gaatgctgaa gcatccaggt
     1561 caaagtcctg accgtctgtt gaacgcgcat cccagccaag gcccaccagg acatttttca
     1621 ttgacggagc tgctttactc agggagacgt tcccgccttt ggaaagagaa acactcataa
     1681 aataacctct tcgattagta attgttcagg ttaacactta aggggattaa ctcccctttt
     1741 cctcagattc aggtttgccc gggaacatga cgcttgcgag aatgcccagc gccagtacac
     1801 ccagtacaac atacaggctg gttgttgccg cgatgctgta accatgatgc cagatgtgat
     1861 cggtcgcatt caggccgagt tttgccgcga tgaagaacag cagcacgata acggccttct
     1921 ccagatgaac caggtactgt ttcagtgcct caaggacaaa atacagagta cgcagaccca
     1981 ggatagcaaa catcatggca ctatagacga taagcggttc acgactgacg gcaatgattg
     2041 ccggtaccga gtcaaacgcg aacattacgt ccgagagttc aaccacagcc acacacagga
     2101 acagcggggt cgcatacagc gccgcttttt taccacggcc aatggtgaca tcgctgtttt
     2161 ctggtttcgc cagttcagca tccacttcct tctggttaag taggaaggca tgccctctga
     2221 gcttcggcca gatagggaag aagcgtttaa ccatacggta agccagatgc tgggagtaat
     2281 cctcaatttc atcatcgtca tcaccgcttt taagcatcat gaccgccgtc caggcaacga
     2341 taatagcgaa gacaacttca acgtacggcc ccagactcag caggctcgta ccgatggcga
     2401 caaagatgcc cctgaagacg atggcaccaa tgatccccca gtagagaaca cggtggcgat
     2461 aacgatccgg aacggcgaac caggagaaga ttgccatcat gacgaacagg ttatcgaccg
     2521 acagcacttt ctccagcgca taacccgtga caaacagact ggcaacctca gcaccgtggt
     2581 ggatatagag gaaaccggca aatgccatcg caaccacaac ccagaatacg gaccagagcg
     2641 cagcactctt cagcgagatt ggcttgtcat cacggtgcat aaacaggtca ataaagatgg
     2701 caccgactga aagcgcaata aaaacaatga ccgtttcagt cgggaagccg atgtgtgtgg
     2761 aaaccatatt ttacccttag ggatgtggat cagaggccaa attcagccgg gtcgaagccc
     2821 aacgcgatag cgatctcgcg ggaggccagt ttctcgtcct gatcgaagtt accgtcactt
     2881 ttcgcaacgg caataccaac acgtaaggcc agctgagcgg cctcaggctg atctttcagc
     2941 gccaggatgt atttcatggt ttcgcccttg ccaatttcaa catcgaaatc gaagcttgaa
     3001 accagtttat tgaagaactc aatcacctca ttggtatcga agaccttcag ctcttctgaa
     3061 gagcgcagaa agcccatcat tttctgcttt tcttccgaac ttacgccgtc actcgatacg
     3121 gcaatacggg cacatacagc aacggtgccc tgcatgaatt ttttgttttt gaaacggcca
     3181 acctggcggg tcagttcgtc acggcctgag ttaatggcac ctttaacttt gtcgaaaaag
     3241 ctcatacctg tttcctttgt tgctgtgtta tttagaaccg gcgctccagc gaaatcccca
     3301 gccaaatgcc cggtccattt cgtcctggcc tttgaagtac tggttaattc gctcgacttt
     3361 aattgcgccg ttttcgttta cgagtctggc aatggcgcac aatgtgcgac ggttctcacc
     3421 ttcggtcagg cgggtctcaa tgggcggttg atccggcaca tgaatggtga ctacaccatc
     3481 agtcttgtcc cagctgggaa caccttcata aataaaggcg taaatcagca cttcgcggat
     3541 atgcttccat tcacgtccat tgatatgcag ccactcgccg tctgatacat cacccgtccg
     3601 gtcatcgccc ttgagctgaa catacggttc atcgcgataa tcaccgaatg cattaccgag
     3661 cgcctgaatg accgatttat acccgtcctg aagctcaaca aaggccccca gatccaggtc
     3721 gattcctttg gagccgaaca tacctgcaaa gcctgatttg ctactgcccc ggtgccagtt
     3781 caggttgatc cggatttcac cgaagttatc ccgtttggtc aggctgattg caggcttctc
     3841 ttttgtcagc gataccttgc tcaggctgat ttttgtctca actggcggag gggttacaac
     3901 cggaggagtt ggggctgcgg gagccggttc gtcggcaata tttacgccga aatgctctgc
     3961 cagcggttta agtccaccgt tgaacccctg cgcaacgaag cggaatttcc agtcattatt
     4021 acgacggtaa aattccccca gaattaaggc tgcttcctga cggccgctca gttcgacact
     4081 gccgctaacc aggccggtag ccccttgctc cacgtcgatg gaaagattac gaagaccgga
     4141 caccgtctgc ccaccgtcac aggtcacggt gaacgcaatt ttctggacat caggtttcag
     4201 ccggttgagc gcgacagtaa aggttgagta ctggccttca ctgaccaggc tgacggtacc
     4261 atcatcgtta cgtggctgac cataaaacac catgtccgca tcaccctgca ccttcccgtc
     4321 ggcatacagg cgaaacgccg aagcatccac ctggccgccc gaggtgatcc ggacccgcaa
     4381 ctcctgggct ggaacggggg cattgccccc cggcgtcagg ttcattagcg aataaccgct
     4441 gaaacgatat cggagtgcat atcgtcaatt gtgcggccgc gacaggcatg gccaagggcg
     4501 gtaaagtccc agttgccgtt attacggcgc agcgaagaaa taacgatccc ggtatgtgag
     4561 ccctgctcag tcagcttgta gcgagccagc tctttgccgg tttgatcgac aacgcggcag
     4621 aaagcgtttt cgacgtcatt gaacgactga ccacggaagc tgtttaccgt aaacgccagg
     4681 tattcaacgt ttgcaggcag gcgggagagg ttgaccttaa tcacttcatc gtcaccgtca
     4741 ccctcaccgg tcaggttatc gccactgtgc acaacagcgc cgcaggtgga tttcagtttg
     4801 cggaaccaga tggtgtcaat tgttttacct gtgctgtcca ttaatacaca gccagcatcg
     4861 agatcgattg agtcgttgcc tccaaacaat cctccaagaa gacctttttt cttaaccgga
     4921 tcccaaccga gaccgaaatg aagctggctt aatgcagatg attctttgct gagtgatacc
     4981 gtctggttct taactaatga aaccatgttt tattccttaa aat
//
LOCUS       DBFIFS010000268         4912 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.268, whole genome shotgun sequence.
ACCESSION   DBFIFS010000268 DBFIFS010000000
VERSION     DBFIFS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.268"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(49..180)
                     /locus_tag="N7A07_004358"
     CDS             complement(49..180)
                     /locus_tag="N7A07_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HGU2478893.1"
                     /translation="MFFVAKHRKIIGVFAFLAPVNPHQNQFPATLRRGQPQNSLNDQ"
     gene            241..315
                     /gene="tap"
                     /locus_tag="N7A07_004359"
     CDS             241..315
                     /gene="tap"
                     /locus_tag="N7A07_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU2478894.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            308..1165
                     /gene="repA"
                     /locus_tag="N7A07_004360"
     CDS             308..1165
                     /gene="repA"
                     /locus_tag="N7A07_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU2478895.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2162..3199
                     /locus_tag="N7A07_004361"
     CDS             2162..3199
                     /locus_tag="N7A07_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2478896.1"
                     /translation="MRPAIFEPQQIIEAGLALQAEGRNITGFALRNRVGGGNPGRLKQ
                     VWDEYISSQTEVTTGPVAELPVEVAEEVKAVSSALAERISQLATELNDKAVRAAERRV
                     AEVTRAAGEQTTQAERELADAAQTVDDLEETLDTLREEHSATLAALDDSRRKEQAQAV
                     ELAQLRERLAATERLLGESEKAGKEAAEQHRQQQADLQKRLDAAGNKLAEATTRHATD
                     LQGVREQYAGEVKELKAQHARNESDLQRRLDAAEKKLAEAITNHAIEIKGIKEQHTGE
                     IKELKAQHARSEKELQNRLDTELKARQAALDKALRDATDAREAKATLTGELQSLKTHN
                     DQLAALLAGKK"
     gene            3375..3644
                     /gene="yacA"
                     /locus_tag="N7A07_004362"
     CDS             3375..3644
                     /gene="yacA"
                     /locus_tag="N7A07_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HGU2478897.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            3644..3922
                     /gene="yacB"
                     /locus_tag="N7A07_004363"
     CDS             3644..3922
                     /gene="yacB"
                     /locus_tag="N7A07_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HGU2478898.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLTAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            4186..>4912
                     /locus_tag="N7A07_004364"
     CDS             4186..>4912
                     /locus_tag="N7A07_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGU2478899.1"
                     /translation="MLIKNKSEIHLGPHFMLNYELLQPGDIILERGYAWYSEKIAKHT
                     NSRYSHAMIYVGGTIIEATRGGGVYSRIPNRSTVRDISDFKVLRLKEFPGKDILQVIC
                     DHARSLCGSQYSVSQALKVKGPDFLRKFANDSRKQFCSRLVAQCYQKAGIQLTENINF
                     CSPGDIERSDYLVEQPEMVYRASEEDVAHAQGESPHTQHTKNAVNFVRNALSIFENYG
                     IKTVGSPDGEVVITTLNDITLAVY"
BASE COUNT         1368 a         1139 c         1296 g         1109 t
ORIGIN      
        1 tgtgatgatg ggcttacatt cttgagtgtt cagaagatta gtgctagatt actgatcgtt
       61 taaggaattt tgtggctggc cacgccgtaa ggtggcaggg aactggttct gatgtggatt
      121 tacaggagcc agaaaagcaa aaaccccgat aatctttcta tgtttggcga cgaagaagat
      181 taccggggct aacaagaaac cgtatagcca acaattcagc tatgcgggga gtatagtcat
      241 atgcccggaa aagttcaaga cttctttctg tgctcgctcc ttctgcgcat tgtaagtgca
      301 ggatggtgtg actgatcttc aacaaacgta ttaccgccag gtaaagaacc cgaatccggt
      361 gtttacaccc cgtaaaggtg ccggaacgct gaagttctgc gaaaaactga tggaaaaggc
      421 ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg gcgcatgccc gttcgcgtgg
      481 tctgcgtcga cgcatgccgc cggtactgcg tcgacgggcc attgatgcgc tgctgcaggg
      541 gctgtgtttc cactatgacc cgctggccaa ccgcgtccag tgctccatca ccacactggc
      601 cattgagtgc ggactggcga cggagtctgc tgccggaaaa ctctccatca cccgtgccac
      661 ccgtgccctg acgttcctgt cagagctggg gttgattacc taccagacgg aatatgaccc
      721 gcttatcggg tgctacattc cgaccgacat cacgttcaca ctggctctgt ttgctgccct
      781 tgatgtgtct gaggatgcag tggcagctgc gcgccgcagt cgtgttgaat gggaaaacaa
      841 acagcgcaaa aagcaggggc tggataccct gggtatggat gagctgatag cgaaagcctg
      901 gcgttttgtt cgtgagcgtt tccgcagtta ccagacagag cttaagtccc ggggaataaa
      961 gcgtgcccgt gcgcgtcgtg atgcggacag ggaacgtcag gatattgtca ccctggtgaa
     1021 acggcagctg acgcgcgaaa tcgcggaagg gcgcttcact gccagtcgtg aggcggtaaa
     1081 acgcgaagtg gagcgtcgtg tgaaagagcg catgattctg tcacgtaacc gtaattacag
     1141 ccggctggcc acagcttctc cctgaaagtg acctcctcag aataatccgg cccgcgccgg
     1201 aggcattcgc acgcctgaag cccgtcagtg cacaaaaaat cagcaccaca tacaaaaaac
     1261 aatctcatca tccaccttca ggtgcatccg gttccccctg tttttgatgc aaaacacctc
     1321 ccacagacgg gtaattttgc ttatccacat ttaactgcaa gggacttccc cataaggtta
     1381 caaccgttca tgtcataaag cgccagccgc cagcgttaca gggtgcaatg tatcttttaa
     1441 acacctgttt atatctcctt taaactactt aattacattc atttaaaaag aaaacctatt
     1501 cactgcctgt cctgtggaca gacagatatg cacctcccac cgcaagcggc gggccccgac
     1561 cagaaactat ttagttacaa cactcaaata caaccaccgg aagaaccccg gtcccgcgca
     1621 gaactgaaac cacaaagccc ctcctcataa ctgaaaagcg aggccgcccc cgcccaaagg
     1681 gcgggaacaa catcgctttt aattatgaat gttgtaacta cattctcatc gctgtcagtc
     1741 ttctggctgg aagtcctcag tacacgctcg taagcggccc tcacggcccg ctaacgcgga
     1801 gatacgcccc gacttcgggt aaaccctcgt cgggaccact ccgaccgcgc acagaagctc
     1861 tctcatggct gaaagcgggt atggtctggc agggctgggg gtgggtaagg tgaaatctat
     1921 caatccgtac cggcttacgc cgggcttcgg cggttttact ccggtatcat atgaaacaac
     1981 agaatgccgc cttacatgcc gctgacgcgg catctgtcaa cttaagtcaa aacaaaaaac
     2041 caacaaattt agtcgttaaa aaatctaaat cctgtaacgg tgtcacgttt ttagttagtt
     2101 tgttagttta tttgtttgtt gtgtataatg aatgcacaat caccagaaca ggagcaggtt
     2161 tatgcgtcca gcaatatttg aaccacagca gattattgag gcaggtctgg cattacaggc
     2221 cgaagggcgg aatatcaccg gattcgcgct acgtaaccgc gttggtggtg gtaatcccgg
     2281 gcggcttaag caggtgtggg atgaatatat ctcgtcgcag acggaagtca ccaccgggcc
     2341 ggtggccgag ctgcctgttg aagtggccga agaggttaag gccgtatcgt cagcactggc
     2401 cgaacgtatc agccagttag ctaccgagtt aaatgataaa gccgtcagag ccgccgaacg
     2461 gcgggtagcc gaagtgaccc gcgcggcggg tgaacagacg acacaggccg agcgcgagct
     2521 ggccgacgcc gcacagaccg ttgacgattt ggaagaaaca ctggataccc tgcgtgaaga
     2581 acacagcgcc acactggcgg cgctggacga cagccggagg aaagagcagg ctcaggccgt
     2641 cgaactggct caactacggg aacgcctggc cgcgacagag agactgctgg gggaatcaga
     2701 gaaggcagga aaagaggccg cagagcagca ccggcagcaa caggcagacc tgcaaaaacg
     2761 gcttgatgca gccgggaata aactggccga agccactacg cgtcatgcga ctgacctgca
     2821 gggtgtcagg gagcagtacg ccggagaggt taaggagctt aaggcgcaac atgcccggaa
     2881 cgaaagcgac ctgcaaaggc ggcttgatgc tgccgagaaa aaactggccg aggccattac
     2941 gaaccatgct attgagatta agggtatcaa agagcagcac actggcgaaa ttaaagagct
     3001 taaggcacaa catgcccgga gcgaaaaaga actgcaaaat aggcttgata ctgaactaaa
     3061 agcgcgtcag gcagcgctgg ataaagcact cagagatgca acagatgcgc gggaagcaaa
     3121 ggcgactttg actggtgaac ttcagtctct gaaaacgcat aacgaccagt tagcagcgtt
     3181 gctggccgga aaaaaataaa tctgcagcgg taaaaccaaa aattcgaaca accgtattgg
     3241 cttacgccgg ttttactcct gtgccatatg caacaacgga gtgccgcctt ccatgccgct
     3301 gatgcggtat atccaggtga cgatatctga atcattatat actgtgtata tacgtagtaa
     3361 tgacgaggtg ataaatggca caggttaata tgagtttaag aatcgacgct gaactgaagg
     3421 atgcttttat ggccgcggca aaaagcatgg accgtaatgg ctctcagtta atccgggatt
     3481 ttatgcgcca gaccgttgaa cggcagcata atacctggtt ccgtgaccag gttgaggcag
     3541 gacgtcagca actcgaacgc ggcgatgtgc ttcctcatga catggtcgaa tcctccgctg
     3601 ctgcatggcg ggatgaaatg agcaggaagg tcgccggtaa atgatggaga tattctggac
     3661 catgctggcc agccaggaca ggaagcgcat tcgtgagtac attgcggagc agaacctgac
     3721 ggccgccatt gaactggacg aacggattgg ttattcagcc agcagcctgg ccgggcagcc
     3781 gtataaaggt cgcaatggtc gtgtggaagg tacccgggaa ctggttattc acccgcattt
     3841 tgttctggtt tatgaggtcg atagccagtg gggaaaagtg tatattctgc gagtgttgca
     3901 tacagcacag aagtggccat aaagaagagt atatttttac cttactgggg gctgattatc
     3961 tgttgttact gttgtggcat acatagaaaa acagttgggt agttaaaata gcataaaggt
     4021 ttgtgaaatg attggaaaat ctatatctcg tttatgccaa aatgaacgca aaaacagcat
     4081 tgcacaatct tgcagtaata atcgagtata aaaggaaaca ctatctgata agtgttttaa
     4141 ctaaagataa gagttctaat agtacaggta ctaaagataa ttaatatgtt aataaaaaat
     4201 aaatcagaaa ttcatttggg cccgcatttt atgcttaatt atgaactcct acaaccagga
     4261 gatattattt tggagagagg ttatgcttgg tattcagaaa aaatagctaa acacaccaat
     4321 agtcgttact ctcatgcaat gatatacgta ggtgggacaa taattgaggc aactagggga
     4381 ggtggggtgt atagccgaat tccaaaccgt tctactgtac gtgatattag cgactttaag
     4441 gtactaaggc ttaaagagtt tccgggaaag gatatcttac aagtaatatg tgaccatgct
     4501 cgtagtctct gtggtagcca gtattcagta agccaggctc tcaaggttaa agggccggat
     4561 tttttaagaa aatttgccaa cgacagtaga aaacaatttt gttcacggct tgtggctcaa
     4621 tgttaccaga aggctggaat acaactgaca gagaatataa atttctgttc accaggggat
     4681 attgagagaa gcgattactt agtcgaacag cctgaaatgg tctatcgtgc atcggaagaa
     4741 gatgttgcac acgcgcaggg ggagtcgccg catacacaac atacaaaaaa tgcagtaaat
     4801 tttgttagaa atgcattgag tattttcgaa aattatggga ttaagactgt cggctctcca
     4861 gatggagaag tcgttattac gacgttaaat gatataactt tagcagtata tg
//
LOCUS       DBFIFS010000269         4863 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.269, whole genome shotgun sequence.
ACCESSION   DBFIFS010000269 DBFIFS010000000
VERSION     DBFIFS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.269"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            77..1411
                     /gene="garP"
                     /locus_tag="N7A07_004365"
     CDS             77..1411
                     /gene="garP"
                     /locus_tag="N7A07_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU2478900.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1427..2197
                     /gene="garL"
                     /locus_tag="N7A07_004366"
     CDS             1427..2197
                     /gene="garL"
                     /locus_tag="N7A07_004366"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU2478901.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKTIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2227..3117
                     /gene="garR"
                     /locus_tag="N7A07_004367"
     CDS             2227..3117
                     /gene="garR"
                     /locus_tag="N7A07_004367"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU2478902.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3214..4359
                     /gene="garK"
                     /locus_tag="N7A07_004368"
     CDS             3214..4359
                     /gene="garK"
                     /locus_tag="N7A07_004368"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU2478903.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4392..4768
                     /gene="rnpB"
                     /locus_tag="N7A07_004369"
     ncRNA           4392..4768
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="N7A07_004369"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1145 a         1171 c         1395 g         1152 t
ORIGIN      
        1 acggcataaa tgaaactcat aaataacatc ttccccataa taataaaaat cagcataagt
       61 acccgaggta aataaaatga ttctggacac cgttgacgaa aaaaagaaag gcgtgcatac
      121 ccgctattta atattactga ttatttttat tgttaccgcc gttaactacg ccgatcgtgc
      181 aacgctgtct attgctggta ccgaagtggc aaaagagttg cagttaagtg cggtttcgat
      241 gggttacatc ttttctgctt ttggctgggc ctacttgctg atgcaaatcc ccggcggctg
      301 gctgcttgat aagtttggct cgaaaaaagt ttacacctac agcctctttt tctggtcgct
      361 attcaccttc ctgcaaggct ttgttgatat gttcccgctg gcctgggcag ggatctccat
      421 gttctttatg cgctttatgc tcggcttctc ggaagcgcca tcattcccgg cgaacgcccg
      481 tattgtcgcc gcctggttcc cgacgaaaga acgtggtact gcctccgcca tctttaactc
      541 ggcgcaatat ttctcgctgg cgctcttttc gcctctgctt ggctggctga ctttcgcctg
      601 gggctgggag cacgtcttta ccgttatggg ggtgattggt tttgtgctga cggcgctgtg
      661 gatcaaattg attcataacc cgacagatca cccacgtatg tctgcggaag agctgaagtt
      721 tatctctgaa aatggcgcgg tggtcgatat ggaccacaaa aagccgggca gtgcggcagc
      781 aagcggaccc aaactgcatt acatcaagca attgctctct aaccgcatga tgttgggcgt
      841 atttttcgga caatatttta tcaacaccat cacctggttc ttcctcacct ggttcccgat
      901 ttatctggtg caggaaaaag gtatgtcgat tctgaaagtg ggtctggtcg cctcgattcc
      961 agcactgtgt ggttttgcgg gcggcgtgct gggaggtgtc ttctcggatt atctgatcaa
     1021 acgcggttta tccctgaccc tggcacgtaa gctaccgatt gtgctgggaa tgttgctggc
     1081 ttccaccatc atcttatgta actacaccaa caacaccacg ctggtggtca tgctgatggc
     1141 gctggctttc tttggtaaag gatttggtgc gctgggctgg ccggtgattt ctgacaccgc
     1201 gcctaaagag attgttggcc tctgcggcgg cgtctttaac gtctttggca atgttgcctc
     1261 cattgtcact ccactggtga ttggctacct ggtaagtgaa ctgcactcct tcaatgcagc
     1321 actggttttc gtgggatgtt cagcgctaat ggcgatggtc tgctacctct tcgtagttgg
     1381 cgacattaaa cgtatggaat tgcagaaata agcaaaggta taagcgatga ataacgatgt
     1441 tttcccgaat aaattcaaag ccgcactggc tgcgaaacag gtacaaattg gttgctggtc
     1501 agcactctct aacccgatta gcactgaagt tcttggtttg gctgggtttg actggctggt
     1561 gctggatggc gaacatgcgc caaacgatat ttcaacgttt attccgcagt taatggccct
     1621 gaaaggcagc gccagcgcgc cagtagtgcg agtgccgacc aacgagccgg taattattaa
     1681 gcgtcttctg gatatcggtt tctataactt cctgattcct tttgtagaaa caaaagagga
     1741 agcagagcag gcggtggcat cgacgcgtta tccgccagaa ggcattcgcg gcgtctccgt
     1801 ttctcaccgc gccaatatgt ttggcaccgt ggcggattat ttcgctcagt cgaacaagaa
     1861 catcactatt ctggtccaga tagaaagtca gcagggcgta gataacgtcg atgctattgc
     1921 cgctaccgaa ggcgtagacg gcatcttcgt cggccccagc gatctggccg cggcattagg
     1981 ccatctcggc aatgcatcac acccggatgt acaaaaaaca attcagcaca tttttaaccg
     2041 tgccagcgcg cacggcaaac ccagcggtat cctcgcgccg gtcgaagccg atgcgcgtcg
     2101 ttatctggaa tggggcgcga cgtttgtggc tgtcggcagc gatctcggcg tcttccgctc
     2161 tgccactcag aaactggctg atacctttaa aaaataacca ccaccgcaac aagagagatg
     2221 attgatatga ctatgaaagt tggttttatt ggcctgggga ttatgggtaa accaatgagt
     2281 aaaaaccttc tgaaagcagg ttactcgctg gtggttgctg accgtaaccc agaagctatt
     2341 gctgacgtga ttgctgcagg tgcagaaaca gcttctacgg ctaaagcgat cgctgaacag
     2401 tgcgacgtca tcataaccat gctgccaaac tcccctcatg tgaaagaggt ggcgctgggt
     2461 gagaatggca ttattgaagg cgcgaagcca ggtacggtat tgatcgatat gagttctatc
     2521 gcaccgctgg caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat tgatatgctg
     2581 gatgctccgg tgagcggcgg tgaaccgaaa gccatcgacg gtacgctgtc agtgatggta
     2641 ggcggcgaca aggctatttt cgacaaatac tatgatttga tgaaagcgat ggcgggttcc
     2701 gtggtgcata ccggggaaat cggtgcaggt aacgtcacca aactggcaaa tcaggtcatt
     2761 gtggcgctga atattgccgc gatgtcagaa gcgttaacgc tggcaactaa agcgggcgtt
     2821 aacccggacc tggtttatca ggcaattcgc ggtggactgg cgggcagtac cgtgctggat
     2881 gccaaagcgc cgatggtgat ggaccgcaac ttcaagccgg gcttccgtat tgatctgcat
     2941 attaaggatc tggcgaatgc gctggatact tctcacggcg tcggcgcaca actgccgctc
     3001 acagctgcgg ttatggagat gatgcaggca ctgcgtgcag atggtttagg aacggcggat
     3061 catagcgccc tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac tcgttaatga
     3121 cgtcgtgccc ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa taagagaaac
     3181 gccctccggc gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc cccagactct
     3241 tataaagaaa gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg atttcgggaa
     3301 atttttcctg atgcacagta cgtttctgtt cctgttgccg acggtggcga aggaacggtg
     3361 gaagcgatga ttgcagccac ccagggttcc gaacgtcacg cctgggttac agggccgctg
     3421 ggcgagaaag tgaatgccag ttgggggatc tccggcgatg gcaaaaccgc gtttattgaa
     3481 atggcggcgg ccagtgggct ggagctggta cctgcggaaa aacgcgatcc actcgtgacc
     3541 acttcacgcg gcacaggcga gttaatcctg caggcgctgg agagcggcgc gacaaacatt
     3601 attatcggca ttggcggcag cgctacaaat gatggcggcg caggcatggt acaggcgctg
     3661 ggggcgaaat tatgcgacgc caacggcaat gaaattggtt ttggcggcgg tagtcttaat
     3721 actctgaatg atattgatat ttccggcctc gatccgcgct taaaagattg cgtcattcgc
     3781 gtcgcttgtg atgtcaccaa tccgctggtg ggcgataacg gcgcatcgcg catctttggc
     3841 ccacaaaagg gagccagtga agcgatgatt gttgagctgg acaataacct ctctcactat
     3901 gccgaggtca ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg cgcaggagct
     3961 gcgggtggta tgggcgcggc gctaatggcg tttcttggtg cggaactgaa aagtggtatt
     4021 gaaatcgtca ctacggcgct gaatctggag gaacatattc acgattgtac gctggtgatc
     4081 accggtgaag ggcgtattga cagccagagt attcacggca aggtaccgat tggtgtcgca
     4141 aacgtggcga agaagtacca taaaccggtg attggcattg cgggtagcct gaccgatgat
     4201 gttggcgttg tacatcagca tggcattgat gcggtcttca gcgtattgac cagcataggt
     4261 acgttggacg aagcattccg cggggcttat gacaatatct gccgtgcttc acgtaatatc
     4321 gccgcgacac tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc aaaccctcta
     4381 tactgcgcgc cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct tcgggggaga
     4441 cgggcggagg ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa cgcctggggg
     4501 ggaaacccac gaccagtgca acagagagca aaccgccgat ggcccgcgca agcgggatca
     4561 ggtaagggtg aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt ccgtggcacg
     4621 gtaaactcca cccggagcaa ggccaaatag gggttcataa ggtacggccc gtactgaacc
     4681 cgggtaggct gcttgagcca gtgagcgatt gctggcctag atgaatgact gtccacgaca
     4741 gaacccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc ggcgggtttt
     4801 tgcttttgga ggggcagaaa gatgaatgac tgtccacggc actataccca aaagaaagcg
     4861 gct
//
LOCUS       DBFIFS010000270         4861 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.270, whole genome shotgun sequence.
ACCESSION   DBFIFS010000270 DBFIFS010000000
VERSION     DBFIFS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.270"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            47..1705
                     /gene="dpiB"
                     /locus_tag="N7A07_004370"
     CDS             47..1705
                     /gene="dpiB"
                     /locus_tag="N7A07_004370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU2478904.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1674..2354
                     /gene="dpiA"
                     /locus_tag="N7A07_004371"
                     /gene_synonym="citB"
     CDS             1674..2354
                     /gene="dpiA"
                     /locus_tag="N7A07_004371"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU2478905.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="N7A07_004372"
     CDS             complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="N7A07_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU2478906.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIIEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            4369..>4861
                     /gene="pagP"
                     /locus_tag="N7A07_004373"
     CDS             4369..>4861
                     /gene="pagP"
                     /locus_tag="N7A07_004373"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU2478907.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQ"
BASE COUNT         1331 a         1094 c         1234 g         1202 t
ORIGIN      
        1 taattacatc cgtcaaagag gctcgggaca acccgcaagg aaaacaatgt tgcagcttaa
       61 cgagaataaa cagtttgcat ttttccaaag actggcattt ccgctgcgta tctttttgct
      121 gattctggtg ttctcaatat ttgtcattgc agccctggcg caatatttta cggccagttt
      181 tgaggactat ttaacgcttc atgtacgcga catggcaatg aatcaggcga aaattattgc
      241 ctccaatgac agtgtcatct ctgaggtgaa aacgcgtgac tacaaacggc tggcgaccat
      301 cgctaacaaa ttacaaagag ataccgattt tgattatgtg gtgattgggg accggcactc
      361 gatccgcctt taccatccta atccggagaa aattggttat cctatgcagt tcaccaaaca
      421 gggcgcgctg gagaaagggg agagctactt cattaccggg aaagggtcaa tggggatggc
      481 gatgcgcgcc aaaacgccaa tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat
      541 tggctacctg gtgagtaaaa tcgatagctg gcgggctgag tttttattac cgatggcagg
      601 cgtgtttgtc gtgctgttag ggattctgat gttgctgtcg tggttcctgg cagcgcatat
      661 ccgtcggcaa atgatgggca tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga
      721 ggcgctgttt agttcggttt atgaagggct gattgcggtg gatccgcatg gttacattac
      781 cgccatcaat cgtaacgcaa gaaagatgct ggggctgagc tctcccggac ggcaatggtt
      841 gggtaaaccc attgctgaag tggtcaggcc cgccgatttc tttaccgaac agattgatga
      901 aaaacgtcag gatgtggtgg ctaactttaa cggtctgagc gttattgcca atcgggaagc
      961 tattcgttca ggtgatgatt tgctgggggc cattatcagc tttcgcagta aagacgaaat
     1021 ttccaccctc aatgcgcaac tgacgcaaat aaaacaatac gttgagagcc ttcgtacatt
     1081 gcgacacgag catctcaatt ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta
     1141 tgatcgcgtg ctggcgatgg tgcaggggga gtctcaggcc cagcaacagc ttattgacag
     1201 cctgcgcgag gcgtttgccg atcgccaggt ggcggggctg ctttttggta aagtgcagcg
     1261 cgcccgggaa ctggggctaa aaatgatcat tgtccccggt agccagcttt cgcaactgcc
     1321 gccaggactg gatagcaccg agtttgcagc cattgtgggc aatttacttg ataacgcctt
     1381 cgaagccagc ctgcgtagcg atgaaggaaa caagatcgtt gaattattcc tcagcgatga
     1441 aggcgatgat gtggtgattg aagtcgccga tcagggctgc ggcgttccag agtctctacg
     1501 agacaaaata tttgagcagg gggtcagtac gcgtgctgac gagcccggtg aacatggcat
     1561 tgggttgtac ttgattgcca gctacgtaac gcgctgcggt ggtgttatca ctctcgaaga
     1621 taatgatccc tgcggtacct tattttcaat ctatattccg aaagtgaaac caaatgacag
     1681 ctccattaac cctattgatc gttgaggacg aaacgccgct ggcagagatg catgctgaat
     1741 atattcgtca cattcccgga ttcagtcaga tattactggc gggaaacctg gcgcaggccc
     1801 gaatgatgat cgagcgtttt aagccggggc taatcttgct cgataactat cttcctgacg
     1861 gtagagggat taatttactg catgaactgg tgcaggcgca ttatcccggc gacgtggtgt
     1921 ttaccactgc agccagcgat atggaaacgg tgtctgaagc cgtacgttgt ggtgtatttg
     1981 attatctcat taagcccatt gcctatgaac ggctggggca aacgctaacc cgtttccgcc
     2041 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
     2101 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
     2161 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
     2221 tggcgcaggc actgaccatc agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca
     2281 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagaccg caacgcatat
     2341 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
     2401 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
     2461 caaaccaaca agcaccggta ccgaggtgcg ttttacgact tcaaacggcg agatcttcgc
     2521 catcccggca accgcaacga ctacgccaga aacgggcgaa agggtacggc ccaggtttga
     2581 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
     2641 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
     2701 cagcattgtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     2761 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     2821 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     2881 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     2941 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     3001 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg caggggcagt
     3061 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     3121 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     3181 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcttgcgccg ccagcaccac
     3241 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagcagctgc
     3301 gccacgactg ataccaacgt ttaccatcac cggaaatagg gttgccatca gcaaaacacc
     3361 cagaccggtt gcggaagaga cggccagaga cattagacag gcgacaaaat aggcggcaat
     3421 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     3481 atcattcgcg ccgatatggg tcatgtaagc ggcaaatcca cacagcatca taatcatcat
     3541 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaatga tatccgtggc
     3601 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     3661 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     3721 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     3781 aatttttcct gtctccaggc cccaaagtaa ataataaaaa attcctaaag cttaaggaaa
     3841 aaatatgccc aataaattgg cgatgaatgc tgattaaaat caagaaaaac tgccattaag
     3901 acattgaagt tgctgttttt atacgtagat aacaaaacgc atatttagcg gttggcaatt
     3961 atcacataat aatgtgtgta aattgatgga ggattgatgc aaatttgtta attgcaatgg
     4021 tgttagctgc ggttgtgtgc tcaaaaaata atctgcaatg ggcgtgggtt gacgatatat
     4081 aatatattcc ctgttaagat ggtttttggt atcttattaa aaaataagtc attttttctt
     4141 ggttgcattt tgttgtcaga aacaactagc tttttctttt atgactattc ccatcgcaga
     4201 aaacgatgtg tcattttaaa tcgatgcgcg gaaatattta acttgaacaa gcggaaataa
     4261 atagagcagc tattcagatt attctttatg ttggggctgt taaggttatg ttaattgtag
     4321 ctttgctatg ctagtagtag atttttgata aatgttttat ggtcacaaat gaacgtgagt
     4381 aaatatgtcg ctatcttttc ctttgttttt attcagttaa tcagcgttgg taaagttttt
     4441 gctaacgcag atgagtggat gacaacgttt agagaaaata ttgtacaaac ctggcaacag
     4501 cctgaacatt atgatttata tattcctgcc atcacctggc atgcacgttt cgcttacgac
     4561 aaagaaaaaa ccgatcgcta taacgagcga ccgtggggtg gcggttttgg cctgtcgcgt
     4621 tgggatgaaa aaggaaactg gcatggcctg tatgccatgg catttaagga ctcgtggaac
     4681 aaatgggaac cgattgccgg atacggatgg gaaagtacct ggcgaccgct ggcggatgaa
     4741 aattttcatt taggtctggg attcaccgct ggcgtaacgg cacgcgataa ctggaattac
     4801 atccctctcc cggttctact gccattggcc tccgtgggtt atggcccagt tacttttcag
     4861 a
//
LOCUS       DBFIFS010000271         4841 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.271, whole genome shotgun sequence.
ACCESSION   DBFIFS010000271 DBFIFS010000000
VERSION     DBFIFS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.271"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            22..450
                     /gene="uspG"
                     /locus_tag="N7A07_004374"
     CDS             22..450
                     /gene="uspG"
                     /locus_tag="N7A07_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU2478908.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(571..2136)
                     /gene="ahpF"
                     /locus_tag="N7A07_004375"
     CDS             complement(571..2136)
                     /gene="ahpF"
                     /locus_tag="N7A07_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU2478909.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2265..2828)
                     /gene="ahpC"
                     /locus_tag="N7A07_004376"
     CDS             complement(2265..2828)
                     /gene="ahpC"
                     /locus_tag="N7A07_004376"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU2478910.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3200..3946
                     /gene="dsbG"
                     /locus_tag="N7A07_004377"
     CDS             3200..3946
                     /gene="dsbG"
                     /locus_tag="N7A07_004377"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU2478911.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4156..>4841
                     /locus_tag="N7A07_004378"
     CDS             4156..>4841
                     /locus_tag="N7A07_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2478912.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGS"
BASE COUNT         1263 a         1188 c         1122 g         1268 t
ORIGIN      
        1 gattaacagg gagaaagggt tatgtataag acaatcatta tgccagttga tgtatttgaa
       61 atggaattga gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt
      121 attcatctac ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct
      181 gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag aagaacgttt gcaaacgatg
      241 gtcagccact tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg
      301 cgggatgaag tcaatgagtt agcagaagaa ctgggggctg atgttgtagt tattggttct
      361 cgcaacccat cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccac
      421 gccaatctgc ctgtactggt cgttcgctaa cagataaaag tatttatgcc gccctgcctg
      481 gaacatcccg gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc
      541 taagcaattg caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc
      601 aaaagcactc agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg
      661 aaccgtcgta cagtcacccg ctgcgaacac gcctttcacg ttggtttcgc atttcgcatc
      721 aatgataatc tcgcccatgc ggttacgttc gactgcgcct tcgagccagt tggtgttcgg
      781 cagcagacca atctgcacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac
      841 acgatctcga tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg
      901 cgcattcaga ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc
      961 ggctttcatt tctggcgcaa attccagcag cgttacgtgc tcaacgatac ccgccaggtc
     1021 aattgccgct tccacgccgg agttaccgcc gccgataacc gctacgcgtt tacctttaaa
     1081 cagcgggccg tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc
     1141 cggaacgttc atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag
     1201 taccgcgcca gaagctgttt caatctgatg cagaccacct tcaactgctg ctgggatcag
     1261 tttgctggcg ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag
     1321 tgcgcctgcc agcttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac
     1381 ggtatcgagg atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg
     1441 tgcggagtaa attgctgccg ctgcacccgc cgggccggaa ccgacgatta atacgtcata
     1501 agcatcacgc ttgttcagct cttctgccgc acgtttttcc gcgcccgtat caattttggc
     1561 aacgatttca gtcaaagtca tgcggccctg accaaactct ttcccgttca cgaacactgc
     1621 cggaacgccc atcacgttac gatcagtgat ctcgttctgg aaggtgccgc cgtcaattgc
     1681 agtgtgcttg atgcgcgggt tcaatacgct catcaggttc agcgcctgta ccacgtccgg
     1741 gcagttatgg caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg
     1801 aatctgctcc agcagagact gcgcttcttt cgacggatga ccaccggtcc acagtaacgc
     1861 cagtaccagc gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt
     1921 agaacctggg ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt
     1981 aaaggtgact ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga
     2041 tttagcgctg tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta
     2101 agccttgagt tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat
     2161 catcatgcaa gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga
     2221 aaatcgggtg ccgcagcacc cgattaatta cgcgattgcg gaacttagat tttaccaacc
     2281 aggtccagag acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca
     2341 cctgggtgag aagctacgta ctgtgctgct ttgattttac gcagcaggtc agacgcgtca
     2401 cggccaatgc cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg
     2461 aaggtcgcac ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg
     2521 gcgccagtcg ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg
     2581 tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt
     2641 ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta
     2701 aagtcagccg ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt
     2761 tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc
     2821 aaggacatct atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttaccga
     2881 ttcggctaat gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac
     2941 aactttgtga actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt
     3001 gaccgtgtaa tctgaactac cctttggtat cttcagcgcc agtctgcagg ctgcgttgtt
     3061 gctcttacaa atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac
     3121 ctgatctaag ctcttaccta tgacaatgat aggttatgcc ttttactcga cttttgcact
     3181 gactgaaaag gacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat
     3241 cgccttcgca gaggaacttc ctgctccagt aaaagcgatt gaaaaacagg gcattacaat
     3301 catcaaaacg ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat
     3361 gggcgtcacc atctacctga ctccagatgg taagcacgct atctctggtt acatgtacaa
     3421 cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg
     3481 acgcgaaatg tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc
     3541 gccggtcatt gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca
     3601 gcaggcgcgc ccgtgggtag attctggcaa agtacagttg agaacattgt tggttggggt
     3661 tatcaaacca gaaagtccgg cgacagccgc ggcaattctt gcctccaaag atcccgcaaa
     3721 aacctggcaa gaatatgaag cctctggtgg caagcttaag ctaaacgtgc ctgcaaacgt
     3781 aagtacagag caaatgaaag tgttaagcga caacgagaaa ctcatggacg atctgggggc
     3841 aaatgtcact ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg
     3901 gttgcccgat cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg
     3961 atggttgaac tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctctg
     4021 ccctaagtgg ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaaacataa
     4081 tcaataaaaa tatatacgca aaaaacattg attaagtgaa tatatcatgg aagaaaaata
     4141 taaccggagt agtgtatgac caatctctac gacttgaaaa agttcgactt aaatcttctt
     4201 gtcatatttg agtgtattta ccagcattta agcattagca aagctgctga atcactatat
     4261 atcaccccat cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct
     4321 ttattcattc gctcaggaaa aggtatcgcg ccgacgacaa cagggttaaa tctgcatcac
     4381 catcttgaga aaaatctcag aggccttgaa caaacaatta atatagtcaa taagtccgaa
     4441 ctaaagaaaa actttattat tcacggccct cagctcattt cttgctctaa taatagtatg
     4501 ctgatccgct gcctacggca agattcctct gtcgagattg agtgccatga tatcctcatg
     4561 tcagcagaga atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa
     4621 atgccagtga tcagccgttc cgtcatttgc atgccattgc atacgattcg caatactcta
     4681 atttgtagta ataggcatcc acgaattact gacaatagca cttatgaaca aattatggca
     4741 gaagagttca cccaattaat atctaaatca gcaggggttg atgatattca aatggaaata
     4801 gacgagagat ttatgaatcg caaaatttca tttcgtggtt c
//
LOCUS       DBFIFS010000272         4806 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.272, whole genome shotgun sequence.
ACCESSION   DBFIFS010000272 DBFIFS010000000
VERSION     DBFIFS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.272"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..491
                     /locus_tag="N7A07_004379"
     CDS             <1..491
                     /locus_tag="N7A07_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478913.1"
                     /translation="SRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSV
                     AVSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEK
                     VKTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEK
                     LG"
     gene            515..1729
                     /locus_tag="N7A07_004380"
     CDS             515..1729
                     /locus_tag="N7A07_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGU2478914.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            1785..2174
                     /locus_tag="N7A07_004381"
     CDS             1785..2174
                     /locus_tag="N7A07_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478915.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            2224..2685
                     /locus_tag="N7A07_004382"
     CDS             2224..2685
                     /locus_tag="N7A07_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478916.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            2669..3232
                     /locus_tag="N7A07_004383"
     CDS             2669..3232
                     /locus_tag="N7A07_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478917.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            3232..3882
                     /locus_tag="N7A07_004384"
     CDS             3232..3882
                     /locus_tag="N7A07_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU2478918.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            3879..>4806
                     /locus_tag="N7A07_004385"
     CDS             3879..>4806
                     /locus_tag="N7A07_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HGU2478919.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTVVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIP"
BASE COUNT         1162 a         1120 c         1444 g         1080 t
ORIGIN      
        1 agtcccgtgt tcagcaatca gcacagcagg cagaaggtaa tggtggtgag gatttatcac
       61 cggttcttga tgcgatgaat gccgtgccgg tgctgaaaac gtggcaggag tccgatccag
      121 atcgcttctc ggttgctgta tccatcgacg ggaagctcca gaatgacccc gcatggaaag
      181 acaaaacgct cactgaacgt ttcgctgaag tggcccgtcg tacgcaggtt gctttcggtg
      241 aagtcagtga gtcgtctgct gacaacaagg cagacaaaac ggatatccgg aaaacggcgg
      301 aagagaaagt gaagaccgct gaacaggagc aggcagtacc tgcttccccg tcagatttag
      361 gcaccacggc ttccgtcgga accggtgata attttgaacg gttacttggc gcttctcatt
      421 cagaggcaga ggcgattatg cgcggtatga cgaatgctga aatagacgcg cttctggaga
      481 agctcgggta acttactgaa ggagaactga agtaatgacg actgtaacat cagcccaggc
      541 gaataagctg tatcaggtgg cgctttttac cgctgccaac cgcaaccgct cgatggtaaa
      601 tatcctcact gaacagcagg aagcgccaaa agcggtttcg ccggacaaga aaagcacgaa
      661 gcagaccagc gcgggtgcgc cggttgtccg tatcacagac cttaacaaac aggccggtga
      721 tgaagtgacc ttcagcatca tgcacaaact ctcaaaacgt ccgacgatgg gagatgagcg
      781 tgttgaaggt cgtggtgagg atctcagcca tgctgacttc tccctgaaaa tcaatcaggg
      841 acgtcacctg gtggatgcag gcggacgtat gagtcagcag cgcacgaagt ttaacctggc
      901 atcctcagcc agaacgcttc tggggacgta ctttaatgac ctgcaggacc agtgtgcgat
      961 agtgcatctt gctggagctc gtggtgattt tgttgctgac gacactattc tgccgacagc
     1021 ggagcaccct gaattcaaaa aaatcatgat caacgatgta ctgcctccga cacatgaccg
     1081 tcactttttt ggcggtgatg cgacaagctt tgagcagatt gaagcggcag atattttttc
     1141 tattggcctg gtggacaatc tctccctgtt cattgacgaa atggcgcatc cgttacagcc
     1201 ggttcgtctg tccggtgatg aacttcacgg agaagatcca tattacgtcc tgtacgtcac
     1261 gccgcgtcag tggaatgact ggtacacctc gacgtccggt aaggactgga accagatgat
     1321 ggttcgtgcc gtgaaccgtg caaaaggttt taatcatccg ctgttcaaag gtgaatgtgc
     1381 gatgtggcgc aatatcctgg ttcgtaagta tgcgggtatg ccgatccgtt tctatcaggg
     1441 gtcaaaggtt ctggtatcag agaataacct gacggcaacc acgaaagagg tcgctgctgc
     1501 aaccaatatt gaccgcgcca tgttactggg ggctcaggcg ctggcaaatg cttacggtca
     1561 gaaggcgggc ggtcacttca acatggttga gaagaaaacg gatatggata accgtactga
     1621 gatagcaatc agctggatca acggtctgaa aaaaatccgt ttccccgaga agagcggcaa
     1681 gatgcaggat cacggcgtga ttgccgttga tacagcagtg aagctctgat tttttccttt
     1741 ccctatgccg ggttttcgcc cggctttttc aggagtcatt aattatggca aagactatcc
     1801 ttgccccgtc actgagtgaa cgggtctata cgggtacgca cggtaatgag tcggtggcag
     1861 aaggcgtatt tacggtgaat gctgcggaag cggacagtgt tattcatctt ctctcactgc
     1921 cagtgggcat ccgtatcaac tcactccaac tggtttcaac gggtggtctg ggtactgcaa
     1981 ccgtcagcat taagtccggt gagcatgctc tcatcgataa cagcgaagct gtttctgcaa
     2041 aatttgccag atatgtgcca gtggagccgt acaccacaca gcgtgacggg gagctggtta
     2101 ctgtcaccat taagactgcc gctgcaaccg gcaccctgaa tgttctgctg cgttataccg
     2161 tggtgggata ctgattaaaa ccttccggcc cgcgtcatgc gggcttttta tccggggaat
     2221 tatatgagtg agaaaattgc cgttgtctat atcggcccaa aacccgtgaa aaaggacacc
     2281 attaccggaa gtcgcacgct gttcccacgt cttgagccgg tgcatgttga cagcgcgatg
     2341 gcctggcaac tgctggggtt tccggatgtc tgggttcgtc atgaagagct tgatgatgtt
     2401 ctgaaaaagc aacaacagaa tgagcagttg cggcaggcac agcaggcgca ggaaagagtg
     2461 cttgctgcgc tggcagaagc ggagaacagt tttgttgttt ctgttaacgg gcaggaggtg
     2521 gatttaagta agctcacctc agcacggctg gcgacgctgt gtgaggcaga agagctggat
     2581 attcacaaag acccgaaaga aacggctgag gcattccgta tccgggtgcg tgaggcattt
     2641 cgccgtcgtg ttgcggagac tgaacagcat ggcggaactg agtgattttt taccgtatgt
     2701 ccgtcgtcat atcagcggtc cactgaacat tatgatgacg gatgctctgt caatggctgc
     2761 cgtggcattc agccgccagt cgttggtgtg ccgtcgggag gttactgttg taccagtagc
     2821 aggaaaagaa atcgtgcttc cgtatgacaa agatgatgag gagtgcgttc atatcatccg
     2881 tatctctgac gataatcatg agctttttgt cggtcgggat gtggatatca gctccggacg
     2941 ctccctgcga tttgcctgtt ctcccggtga ggtgagcgtg ctttatgccg tcgctccgaa
     3001 agccggacgc agccagatac cggatgaact cctcacatgg cctgaagaag tggctgcggg
     3061 ggcacttgag cggttgttca tgcagactgg tgtttcatgg tcagatccgt tacgcgcaca
     3121 gtatttttct gtgcagtttt ctgaggggat ccgtcgggca tatcgtcata cactggcgac
     3181 aagcccgtac tcttcatacc gcaaccctgt acgcaggcag aggtttttct gatgacgacg
     3241 attacagaaa tcatcggacg tgtgaataca caactggttg acccgatgat ggttcgctgg
     3301 cccctgcagg aattgtgcga ttattacaat gatgctgtga gggcagtgat tctggcgaga
     3361 ccggatgctg gcgcaagcct ggaaacaata agttgtgttc ctggcgcccg tcaggttttg
     3421 cccgatggtg taatacaact tcttgacgtg atatgcctca gtgacggtag tgcagtcaga
     3481 ccattatccc gggaggtgct ggatgcgcag tatcctgagt ggcccacaat gaagggtatt
     3541 cctgaatgtt ttatcagcaa cgacctgtcc ccgcgcgtat tctggctgtt tcctgctcct
     3601 gacaaagaga taagtattga tgcagtggta agccggatac cggaggcagt gtatgttctg
     3661 acgcaggacg atgatacgcc agttccactg gaagaggctt atgttaaccc actggtggag
     3721 tggatgttgt ttcgcgcttt cagtaaggat gctgccggtg gcgcagaatc ggggctggct
     3781 gcgcagcatt atcagagttt tgttgagcaa cttgggatca aacagggggc agacagtgca
     3841 ttgtctgccc gtaaaaaagt gtttaacgga ggtggagtgt gagtgttgtt gtttcgggga
     3901 cgctgaaatc tcctgatggt gaggcgatat caggagcaaa tattaccctg acggcgctga
     3961 cagtttcacc ggatgcgctc agcggcacca gtgcgtcggc agtgacccgt gaaggtggat
     4021 attacggaat gacgatggat ccgggggagt atgcggtttc ggtgacggtg aaagggaaga
     4081 ctgttgtcta cggacgtgtg cgtattgagg ggaccgaaag tacggtgacg ctcaatatgc
     4141 tgttacgccg cagtcttgtt gaggttagca tacccggaga actgctgaca gatttccggc
     4201 agatacagaa taatgtggct gatgaccttg ccactattcg tcgcctgaat gaagacacgg
     4261 cgacaaaaaa cactcaggcc acacagtcaa aagaaagtgc agcagccagt gcgaagagtg
     4321 catctgacag tgcaaagacg gcaaccagca gggcggctga agccggacaa aaagcgactg
     4381 atgccactga ggctgcgacc cgtgcagtca cagcagcggg gaatgcagag gaaagctcga
     4441 cccgtgccgg agagtctgaa aaagccgccg gagctgatgc agaaaaagcc agacagcatg
     4501 ctgaaaaggc caggctggcg caggagagcg ccggagagat ccttaagcgg gcagaggctg
     4561 ccactgtcag tgctgaagag gccagacgta tggctgagaa tgcacggggg ccccgggggc
     4621 ctcagggaga aactggtccg aagggggatg tcggtcctaa aggcgaaaca ggtccagtgg
     4681 gccctcaagg gcccgcaggg ccgaaaggtg agcgtggtga cgttggtgct cagggggctg
     4741 tagggcctgc tggtccgcgt ggtgagaagg gcgaacaggg ggagcgagga ccgcagggaa
     4801 taccag
//
LOCUS       DBFIFS010000273         4777 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.273, whole genome shotgun sequence.
ACCESSION   DBFIFS010000273 DBFIFS010000000
VERSION     DBFIFS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.273"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            153..719
                     /gene="slp"
                     /locus_tag="N7A07_004386"
     CDS             153..719
                     /gene="slp"
                     /locus_tag="N7A07_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU2478920.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            875..1405
                     /locus_tag="N7A07_004387"
     CDS             875..1405
                     /locus_tag="N7A07_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU2478921.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1466..2494)
                     /gene="chuS"
                     /locus_tag="N7A07_004388"
     CDS             complement(1466..2494)
                     /gene="chuS"
                     /locus_tag="N7A07_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HGU2478922.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(2543..4525)
                     /gene="chuA"
                     /locus_tag="N7A07_004389"
                     /gene_synonym="shuA"
     CDS             complement(2543..4525)
                     /gene="chuA"
                     /locus_tag="N7A07_004389"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HGU2478923.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1275 a         1167 c         1079 g         1256 t
ORIGIN      
        1 ccacgcccca aaataacgga attgaactaa acagaaggga tataaacatc agacaggttt
       61 acgttactat caggcatatc gcctcagaat cagatgaaaa ctataaagaa atatctatta
      121 tggttttaat atttgttgat aaggatagta acatgaacat gacaaaaggt gcactcatac
      181 tcagcctttc atttttgctt gcagcatgta gttcaattcc gcaaaatatc aaaggaaata
      241 accaacctga tattcaaaaa agttttgttg ctgttcataa ccagccgggg ttatatgttg
      301 gtcaacaagc gcgctttggt gggaaggtta tcaacgttat caatggcaaa acggatacgt
      361 tgttagaaat cgctgtatta ccgttggata gctatgcgaa gcctgatatt gaagccaact
      421 atcagggccg actgctcgcc agacaaagcg gcttccttga tccagtgaac tatcgtaatc
      481 actttgttac catcctcggc accattcagg gtgaacaacc tggctttatc aataaagtcc
      541 cgtataactt cctggaagtg aatatgcagg gcatccaggt gtggcatttg agagaagtgg
      601 ttaataccac ctataacctg tgggattacg gctatggtgc attctggccg gaaccgggct
      661 ggggtgcgcc ttactacacc aatgcggtga gtcaggtaac acctgagctg gtcaaataac
      721 accaccgaaa gatgcagatg ctttctcagc atctgcatca tgcattacat caaattaata
      781 cacagtaagc taactattat tattataagc cctgtcctgt taattacctt tggcaaactg
      841 attataaagt taatgtccgc accaggagtc ggttatgttt cttataatta ccagggatac
      901 gatgttcttc accgcgatga aaaacattct gagtaaaggt aatgtcgttc atatacagaa
      961 cgaagaagag atcgacgtaa tgttgcatca gaatgccttc gtcattattg atacattaat
     1021 gaataatgta tttcattcta attttctcac tcaaattgaa cgattaaaac ctgtccatgt
     1081 cattattttc tcccccttta atattaaacg ctgcctgggg aaagtgccgg tgacctttgt
     1141 tccgcggact atcactatca ttgattttgt cgcactcatc aatggcagtt actgctctgt
     1201 gcctgaagcg aatgtgtcac tttcgcgcaa gcaacatcag gttctgagct gcattgcgaa
     1261 tcaaatgaca acggaagata ttctggagaa actgaaaata tcgctaaaaa cgttctactg
     1321 ccataaacac aatatcatga tgatcctcaa tcttaagcgg atcaatgagc tggtacgcca
     1381 tcagcatatt gattatctgg tgtgaacgat tgatcaatat aaagaggccc agcaataaat
     1441 agccagacct cccgttaatt atacgttatg cagcaacgcc ttccggtatc agcgaagcaa
     1501 tttgcttacg ccattgcgct tgctcctgtt cgccttctgt acgttgacca taaagttgcg
     1561 ctatctgcgt accatcatgg gcaaacaatt ccagactggt tacgtagcca tcgctggtcg
     1621 gtttacgggt aacccaggct tcagcaatgc tctcttctaa tagatgaaga gtaaacgtcg
     1681 ggttgaaaat attcagccaa cctttcattg gcaccacttt ttctaccaca ccggtgaaaa
     1741 tctgtacgca gccacggttg ccaacaaaca ccatgatttc attaccatcc tgctgtgcag
     1801 attcaagaat ttgcgccaac gcactgttgg atactttgca ggccaaatcg tctgccacca
     1861 gattgaacgc ctgttggcgc gtcaggttgt ggcgcttgag caacgtaaaa aactgatgaa
     1921 cgtcggtcat cgcccgccac tcttgctcga ccacagtggc atcggctcgc gtttgaacaa
     1981 ctggcgcatc aacggctttt aactcaagcg gcgtattctc atcggtgata aaccgtgcca
     2041 gaagctcact ccatgccgcc atatcggtat tgtcggtggc ataaactttt agtaatgcat
     2101 cgccctgatg atcaaagaac tgaatactct ggcgttcgcc acgagccgtg ttttctttga
     2161 tgtggaaaac actggcccat tgattgagaa acagacgtaa atccagcgcg cgcggattga
     2221 ggatcaatcc ggcatgtccg ttcaaatgct ggtttgtgaa cgtaccaact tgctcatgga
     2281 ctgcatattc attacgacaa atacatttgg tttcgccaac actttcgagc gccgccagaa
     2341 tttcacggat atcgccgtgc atccgccacg catcgtgcgt gacgcgtgca aatgccagtt
     2401 ctgcttctct aatattcatt aaccctgcga tgtcacgcgc gtactttccg ggattttgtt
     2461 cttttaactc aagccagcgt gtgtagtggt tcattgtctc tcttccttcc agataaatgc
     2521 cccgaaatat cggggcagat gattaccatt gataactcac gaaaattttt ccgttacgac
     2581 catcctgtgg gatgccttgc ggcgaccagt actctttgtc gaaagcgtta cccaacacca
     2641 aagtagtggt catacctttg agcgcctgtt gtccttgata actgacgtag aaatcattca
     2701 cgccatagcc tggttgtttg ctgtaactgc tgctgatatg tgttgagcga tcggcaaacg
     2761 taccaaccca cccaacagag aagccactgt gagcgatcgg aatattcaga gtgctggtaa
     2821 cagtatccgg gttaatgctg gagatgtatt cgccggtatc ggtgtctttg ccgcgggtac
     2881 ggttataggc cacatcaagg ctaaacagat cagtggtata tttcgtcatc acatcccagc
     2941 cccagatttt ggcgttcggg acgttatacg acatagtcgt cgccgccgcg aaatcgacgg
     3001 tcgtggagat gtaatccttc gctttggtat caaagtagct ggctttaaat tccagagcat
     3061 cattggacaa catcaggtca tcaaaacgca gcccaaaacc gtactcctga gtttcgttag
     3121 tttccggacg taagttcggg tttggcaccc aatagttggt atagaagcga ccaatcgaga
     3181 agtgcttaga atcgttatac atttcgccca tcgtcggggc gcggaatgcc tgggcatatg
     3241 agccaaataa catcagccag ttagtcggat tgatagtcat ccccgcacga gatgaccatt
     3301 tgtcggcatc aacatctttg taaccgtcac tgctaccgcg ataactgtca tagcgggttc
     3361 cgccaagcag ggtaatcggc agatcgcgta aggtgatctc atcctgtagc cagccggagc
     3421 taaaatcgat ttttgcttgc gggaagcccg tcgtcgcgcc gcccggatgt tgttcctgac
     3481 gataatactc accgccatat gtcagtaagt gagaagcgaa actgtcggca aagagagtgg
     3541 aacggttctc cagcctggct ccttttgtta tctgttcacg atactcgccg gaactccccg
     3601 tgttttgcgc attaatacgg acttccgacc aataaatttt tgcatctgca tttaaccagt
     3661 cgttgccctg cggggcgagt ttataagaaa gctgcgcatc gcgttgaatt gttgaacgat
     3721 caaccatcgg gttgctgctt tcagaagcct caacggtctg cggatttttt ggttcacgcg
     3781 cgtcgttgtt gtagtaacgc actaaaccgc tcagagactg ggctgaatca atttgccagg
     3841 tccctttcgc cagcatgtta ttaatggact cgtcattcgg cgcggtttca ccattgctct
     3901 ggcgtaaatc accccgatcg cgactggacc aggccacaat accatccaga ttttcagttc
     3961 gcccaaacgc gctcgcgcct aatcccaggc tatggtcccc cgtgccgcca gtaccaaaga
     4021 cacgaaaacc actgctttgt ccttcctgca ataaatcttt tgcatcgacc gtatcgtagg
     4081 agatcactcc acccagcgcg ccactgccat acagtaatgc tgaaggtcca cgaacaatct
     4141 caacacgctt gatcagcgcc ggatcgagaa aagtgccatt caggtgtccg gtatccgttc
     4201 cctgacgaac accatcgaca agaaccagca cgccgcgatg atcatagcca cgcatattta
     4261 catcctgacc gttggttcgt ccggtaccat ccagagtaat tccaggaaca tgacgcagca
     4321 gatcggtggc tgaagtagcc gtttgatttt caggagcgga agtgtcgatg acgctgacca
     4381 tcataggcgc ttcgaaggaa ctacgggcat tccccgttgc cgtaacggtc atggtttcag
     4441 tagcaaaagc aaacgttggc aaggtggcag aaacagctaa ggccaataaa ctcaaacgca
     4501 acgaggtaaa ttgcggacgt gacatagcga ttctccatga ggaaaataaa aaatgctggc
     4561 tgcctgggtt aataccgggg tggctggcga aaaataaatt tatttaaaat tcagagcgat
     4621 aatattcgcc gggtgagttt acaagcgttt atatcccatg aaattgcctg ataactcggc
     4681 aattacgcaa aagcacgccc tctttgtcag gcaaaacgat atgccccatc aggagtgaag
     4741 agagctgtgc gttatccctt gcaaatgcta tggtttt
//
LOCUS       DBFIFS010000274         4710 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.274, whole genome shotgun sequence.
ACCESSION   DBFIFS010000274 DBFIFS010000000
VERSION     DBFIFS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.274"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            131..274
                     /locus_tag="N7A07_004390"
     CDS             131..274
                     /locus_tag="N7A07_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478924.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(493..2031)
                     /gene="aldB"
                     /locus_tag="N7A07_004391"
     CDS             complement(493..2031)
                     /gene="aldB"
                     /locus_tag="N7A07_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU2478925.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2139..3434)
                     /locus_tag="N7A07_004392"
     CDS             complement(2139..3434)
                     /locus_tag="N7A07_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGU2478926.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3564..>4710)
                     /gene="yiaY"
                     /locus_tag="N7A07_004393"
     CDS             complement(3564..>4710)
                     /gene="yiaY"
                     /locus_tag="N7A07_004393"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU2478927.1"
                     /translation="SSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTKLG
                     MAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCA
                     KGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIKMA
                     IVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAV
                     TMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHG
                     VCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDI
                     PAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1130 a         1249 c         1141 g         1190 t
ORIGIN      
        1 atgatgcaat gcaatttcct tatttatata acgacatatt gttttttata tactttctga
       61 agtgtgctgc gtggcttttt aacaggtcga ggcttatcat attgaaataa tggaactatc
      121 agccaaacta atgaataact tcagtaaaac atccacaatg cagattaagc gtattaaacc
      181 ttcaataatc taccgtttat tgctgattgt cctcggaata ccaatggtga tttacggtct
      241 ggtttgcccg ctcaccatcg aaacgcgaga ttaaactcct ggcaaaacac gccccagaaa
      301 cgcacttttg ttggcgtagt ctgggttatt gcacacgtag gtttaaaacg taaattgtaa
      361 caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc ttatccggcc tacggtcagg
      421 tccccatagg catgataaga cgcgtaagcg tcgcatcagg caattaatac ccgatgcgac
      481 cagcttctta catcagaaca gccccaacgg tttatctgag tagctcacta gcaggcactt
      541 ggtttgctgg taatgctcca gcatcatctt gtgggtttcg cgaccgatac ctgattgttt
      601 gtagccacca aacgccgcat gtgccgggta agcatgataa cagttggtcc acacgcgccc
      661 agcctgtatg ccgcgcccca tcttataggc cagattaccg ttgcggctcc agacgcccgc
      721 gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg ttttgaaggt
      781 agtcaccgcc agcaccgggc caaaaatctc ctcctggaac acgcgcatat tgttctgacc
      841 aaacagaatc gtcggttcga ggtagtagcc gtctttcagt tcaccttcca gcagcttgcg
      901 ccgcccgccg gtgagcacat cagcgccctc ttttttaccg atatcaatgt agttgaggat
      961 ggtttccagt tgcccgtgag aaacctgcgc acccatttgc gtcacgctgt cgagtgggtt
     1021 accgctgcga atgctttcga cacggcggat ggcgcgttcc ataaagcgtt cgtagattga
     1081 ctcctgcact aaggcacgac tcggacaggt gcaaacttcg ccctggttaa aggcaaacag
     1141 tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca catcagcaaa
     1201 gaagatattt ggcgacttac cgcccagctc cagcgtcacc ggaataatgt tttgcgtggc
     1261 gtattgcata atttgttgac ccacttccgt tgagccggta aacgccactt tggcgatgcg
     1321 tttcgaggtc gccagatatt cgccaatttc cccacctgca ccgttgacca cgttcaccac
     1381 gcccggcggc agtaaatcac cgacgatttc cattagcagc agtacagaaa gcggggtaag
     1441 acgtgcgggt ttcagcacca cacagttgcc cgccgccagc gcgggagcca ttttccagct
     1501 cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta acggttcatg
     1561 gaaatgatag gccacggttt cgctatcaac ttcactgatc ccaccttcct gcgcccgaat
     1621 acacgaggcg aaatagcgga aatggtcaat cgccagcggt acatccgcag cactggtttc
     1681 gcgaatgggt ttgccgttat cccaggcttc agctgtcgct aacagctcga ggttttgttc
     1741 cattcgatcg gcaatcttaa acagaatcgc cgcgcgatcc tgcaccgagg tgtgcgccca
     1801 tttatctttc actttgtgcg cagcatccag cgccagatcg atgtctcgtt tgcccgaaga
     1861 cgccacttcg cacagcagct gcccggtcac cggcgtcagg ttctggtaat actcaccatc
     1921 ggcaggggct acccattcgc cgccaataaa gttgtcatag cggtttttta acttgagggg
     1981 gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca tgatcgtctc
     2041 cttgcggtgt gaggtataac aagggtagac gtggctggcg aaatcttcgc cagtcggtaa
     2101 cagcttttcg acggctatca cgaatttacg ggcaagggtt acagcggata atgcgcgaag
     2161 tacacggctt gctggtcatt aattttcttc tcttgcgcaa acccttcttt aacaattgtc
     2221 cgtaaatcag ctcgaaccgt attatcagag acgcccaggc tcgcgctaat ttcagcaact
     2281 gtgtactctt tccccggatt ggcgagcatc acattcagga gcgttacctg gcgcggattc
     2341 aggcgtttaa gcacaccaga gctaaacagc acactatcga gttttgcgct tcgttgtgta
     2401 atttcatcga tatgctgctg ccagttttgc agagcacgtt taatgacacc cgcctgatat
     2461 tcaaggaaat aagtcagatc cataccatct gattcagtgt actggtagct ggcggcatat
     2521 ttgaccggtg cagcatgaag caaacggctg atggaaatat atttaaagac atcgtagccc
     2581 gatttcagca tataccagta aaacagcgcc cgactggtgc gcccgttgcc atcccgaaac
     2641 ggatgttcat gggccagcat aaaatgcaga atgcaggcac ggaccaacgg atgaatatac
     2701 ccttcatggc tgtttaacca ctggcaaact ttttccagcc gttccggtaa caatgccgca
     2761 gcgggcggtt gatgcactat attgccatca tagtcggcga tcacaacatc atctgtttca
     2821 cgaaattctc ccggacgata tttcgcgtca ttgattccac ccataccaat ggcatgtaac
     2881 tggcgaataa gcgcaggtgt cagcggttct gcgagtaagt gcggaatttc cgccatcagc
     2941 cgggcatttc ccgcaatcat atgctcgtct tcagtgcgtg gcgaccgtcc tgtttccagc
     3001 atttttctcg ccaccagcgt tgtagtattc gcaccttcca gctgggagct ggtgatcggc
     3061 tcttccaggc gtaactggct taactcggct ccggccccgc gtaaccgttc agtctgatct
     3121 ttcagcgcca gcgcggtagc acgtttgtcg acaagctcgc acgcttctgc aatcaccggg
     3181 gtaatgttga atccggcctg ttcacccgct tcattgcaat aattaattcg ttggatcgcc
     3241 gcgttgcgcg ccagacgggt aagcgtccac gccacatcga cgttttctcc ttttttgatg
     3301 cggtactgca actcatcaaa aggcagataa cgtccttttt catcggtaac ccggttaaag
     3361 cgcaggtggg cctggagttc ctcacccgat aagtgtgata aaggaatcag cgtgggaggt
     3421 gatttgatag ccatttgctt atctcaaaaa agcgcaatat tgagatagtg tatagcagga
     3481 aagagaaact atctcaaaaa aagcaagttt tgagatagtt gaaaaaatgg ctgacaattt
     3541 gccagccgtt gtggaaatga tgattacatc gctgcgcgat aaatcgccac aatttcttcg
     3601 tgagttgcct ggatcgggtt agtaaagcca caggcatctt tcagggcatt agccgccaga
     3661 accgcgaaat cttcttcttt cacgttcagg tcgcgtaggc ctgccgggat atccactttc
     3721 ttcgccagtt cacggatggc gttaatgcag gcttcagcac cttccgcgtc gtttttacct
     3781 gtcacgttca cgcccattgc agcggcacag tcacgcagac gtgcagcggc gactttgctg
     3841 ttgaatacct gaacatgcgg cagcagaacg gcgttacata caccgtgtgg caggttgtag
     3901 aaaccgccca gctggtgcgc catcgcatga acataaccca gagaagcatt attgaacgcc
     3961 ataccggcga ggaactgggc ataagccatt gcttcacgcg ctttcgcatt actgccatct
     4021 tcaacggcta acggcaggtt ttcggcaatc atggtcacgg ctttcagtgc acaagcgtca
     4081 gtgatcggcg tggcggcaat agaaacatac gcttcgatag cgtgcgttaa ggcatccata
     4141 cccgttgcgg cggtcagtga cttcggcata ccaatcatca gagaggagtc attgacagaa
     4201 agcagcggag tgacatgttt atcaacaatc gccattttga tatgacgcgc ttcgtcagtg
     4261 atgatgcaga aacgggtcat ttcagaggcc gtacccgccg tggtattgat ggcgatcatc
     4321 ggcagctgcg gttttgcaga gcggtcaacg ccttcgtaat cgcgaatatc gccgccattg
     4381 gctgccacca gcgcaatacc ttttgcgcag tcgtgtggag aaccaccgcc taaggagatc
     4441 acgctatcgc aattattctc tttaagtaat ttcaaacctg cggcgacgtt ttccgtggtg
     4501 gggttaggtt gggtgccatc ataaataacg ctaaaaatat tgcgttcttc cagtgctttt
     4561 tgcacatcgc ccgccatacc taatttcgtt aacatactgt cagtgacaat taaggtacgg
     4621 gtaaatccat aatctgccat catattcatt gcatcagtca atgaatcagc gccgatgaca
     4681 ttcacagaag gaataaagaa agttgaagat
//
LOCUS       DBFIFS010000275         4694 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.275, whole genome shotgun sequence.
ACCESSION   DBFIFS010000275 DBFIFS010000000
VERSION     DBFIFS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.275"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..500
                     /locus_tag="N7A07_004394"
     CDS             <1..500
                     /locus_tag="N7A07_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478928.1"
                     /translation="TIAAGNNGTARFYGTIEDSPCSIVPDDHKLEVDMGDIGSGILKN
                     NGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTNSGNYYTIYNTDTGAAFNNVSLA
                     IGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDFKAWLVGAADAPDLGNFEANTTF
                     QITYL"
     gene            582..3221
                     /locus_tag="N7A07_004395"
     CDS             582..3221
                     /locus_tag="N7A07_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU2478929.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            3244..4002
                     /locus_tag="N7A07_004396"
     CDS             3244..4002
                     /locus_tag="N7A07_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2478930.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            4013..4513
                     /locus_tag="N7A07_004397"
     CDS             4013..4513
                     /locus_tag="N7A07_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478931.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            4510..>4694
                     /locus_tag="N7A07_004398"
     CDS             4510..>4694
                     /locus_tag="N7A07_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478932.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVP"
BASE COUNT         1180 a         1197 c         1263 g         1054 t
ORIGIN      
        1 caacaatcgc cgctggcaac aatggtacag cacgtttcta cggcaccatt gaagattccc
       61 cgtgctctat cgttcctgat gatcacaaac tggaagttga tatgggtgac attggttcag
      121 ggatcctgaa aaataacggg acttctacac cgaaagcttt ccagatccat ctgcaagact
      181 gtgtgtttga cacccagaca acgatgacca ctaccttcac cggtaacgcg tcttctacca
      241 acagcggcaa ttactacacc atttacaata ccgatactgg tgcggcattt aacaatgtca
      301 gcctggccat tggtgacgct cagggaacct cttataaaag cggcgcgggt atcgaacaga
      361 aaatcgtaaa cgatacggcg accaacaaag gcaaagcgaa gcagacgctg gactttaaag
      421 cctggctggt gggcgctgct gatgcgccag atttaggtaa ttttgaagcc aacacgacct
      481 tccagattac ttatctctaa gtgatacccc catagctttc gggctatggg gtttttaaat
      541 ggaattcgcg caaattataa taattacgga tggatacgtg tatgcctgac cattctcttt
      601 tccgactgcg ggtacttccc tgctgcgttg cattggcaat gtccgggagt tatgtcaatg
      661 cctgggctga aaatgaaatt cagtttgatt cccgttttct ggagttaaaa ggcgacacaa
      721 aaatcgatct gaagcgattt tccagccagg gttatgtcga acctgggaaa tacaatttac
      781 aggttcaact aaataaacag ccgctgacgg aagaatacga tatttactgg tacgcctctg
      841 agaacgatgc cagtaaaacc tatgcctgcc tgacgcctga actggtcgcg cagtttggct
      901 taaaagagga tgtggcaaaa aacctgcaat ggatccacga cggcaaatgc ctgaaacccg
      961 gtcaactgga aggcattgat attaaagctg acctgagtca gtcagcgtta gtcatttcat
     1021 taccccaggc ttaccttgaa tataccgaca tcaactggga tccgccttca cgctgggatg
     1081 acggtatatc tggtttaatt gctgactaca gtattaccgc ccagacacga catgaagaaa
     1141 atggcgggga tgacagcaat gaaattagcg gtaacgggac ggttggggtg aacctcggcg
     1201 catggcgtct tcgtgccgac tggcagactg attatttgca tagtaaaagc aatgatgacg
     1261 atgttatcaa cggtgatgac acgcaaaaaa actgggagtg gagccgctac tacgcctggc
     1321 gagccttacc gtcgctaaaa gccaaacttg gccttggcga agactacctg aattctgata
     1381 ttttcgacgg ctttaactac gtgggtggca gtatcagcac cgacgatcaa atgttgccgc
     1441 cgaatctgcg cggctatgcg ccggatatct ccggcgtggc gcacaccacc gcgaaagtga
     1501 ccgtcagcca gttgggccgc gtcatctacg aaacccaggt cccggcgggg ccgttccgca
     1561 tccaggatct tggcgattcg gtctccggta cgctgcatat ccgcattgaa gaacagaacg
     1621 gtcaggtgca ggaatatgac atcaacaccg cctcgatgcc gttcctgact cgccccggcc
     1681 aggtgcgcta taaactgatg atgggccgcc cgcaggagtg ggggcaccac gtggaaggcg
     1741 gtttcttctc cggcggcgaa gcttcctggg ggattgccaa cggctggtcg ctatacggcg
     1801 gggcgctggc agatgaacac tatcagtcgg cggcgcttgg cgtcggtcgc gacctgtctg
     1861 tgtttggtgc ggtggccttt gatatcaccc actcgcatac ccgtctggat aaagagaccg
     1921 cctacgggaa aggttcactg gacggcaact cgtttcgcct gagctattcc aaagacttcg
     1981 atgaactgaa cagccgcgtc acttttgccg gataccgctt ctcggaagag aacttcatga
     2041 ccatgagcga gtatctcgat gccagcgaca gcgaaatggt gcgcaccggc aacgacaaag
     2101 agatgtacac cgccacctat aaccagaact tcagggatgc cggtgtgtct gtttatctca
     2161 actacacccg ccatacctac tgggatcgcg acgaacagac caactacaac gtcatgctct
     2221 cgcactactt caacctgggc agtatccgca acatgagcat ttccatgacc ggataccgct
     2281 acgagtatga caaccaggcc gataaaggtg tgtacatatc gctcagtatg ccgtggggtg
     2341 acagcagcac catcagctat aacggcaact acggcagcgg ttcggacagc agccaggtgg
     2401 ggtatttcag ccgtgtcgat gacgcaaccc attaccagtt gaacgtaggc accagcgaca
     2461 atcactccag cgttgacggt tattacagcc acgacggatc gctggcgcag gtcgatctca
     2521 gcgctaacta ccatgaaggg cagtacacct cggcgggtat ttccttacag ggcggcgcga
     2581 cgctcaccgc acaaggtggc gcgctccacc gtacccagaa tatgggcggt acgcgtctgc
     2641 tgattgatgc cgacggtgtg gctggtgttc cggtggaagg aaatggcgcg gcggtttaca
     2701 ccaatatgtt cggtaaggca gtggtggcag acgtcaacaa ctactaccgc aaccaggcgt
     2761 atatcgacct aaacaacctg ccggaaaacg ccgaagccac ccagtccgtg gtgcaggcca
     2821 cgcttaccga aggggccatt ggctaccgta agttctcggt gatcagcggg caaaaagcga
     2881 tggcggtgct gcgtctgcaa gatggcagtt atccgccgtt tggcgcggaa gtgaaaaacg
     2941 acagcgcgca gaacgtcggt ctggttgacg atgacggcaa cgtctacctc gcgggcgtaa
     3001 aacctggcga gcatatgatc gtttcatggg gcggtgtggc ccactgcgat attcatctgc
     3061 ctgacccgct gccagccgat ctgttcaatg gcctgttatt accatgccag caaacagggg
     3121 cgatatctcc ttcgatgcct catgaaatta agccggtgat ccaggagcag acccagcagg
     3181 tgatgccaac ggaagcgcca gtatcggtat cagccaatta ataacgtgat taaggaatga
     3241 tccatgacga atctattttg ctctgccaga acggccgcaa cgacgctggc gttactgata
     3301 actgccgcca gcctgtcagt acaggcctct gtcacgccgg accgtacacg cctgattttt
     3361 aacgaaagtg ataaatcaat cagcgtcacg ctgcgcaata acgacccgaa aatgccgtat
     3421 ctggcacaaa gctggatgga agatggacag ggtaacagga ttagctcgcc actgacggtc
     3481 ctgccgccag tgcagcgcat cgattccatg atgaacggtc aggtgaaggt acagggcatg
     3541 ccggatatca acaagctgcc tgccgaccgc gagagcgtgt tctatttcaa cgtgcgtgag
     3601 atcccgccga aatcgaacaa acctaacacg ctacagatag cgctgcagac gcgcattaag
     3661 ttgttctggc gaccaaaggc gctggaaaac gtcagcatga aaaacccgtg gcagcataag
     3721 gtgaccttaa ccctcagtgg tcaggagttt accgtcaata acccgacgcc gtacttcgtg
     3781 gttatcagta acgccagtgc gcagaaaaat ggcaatccgg cagcgggatt ttcccccatg
     3841 gtgatatcgc caaaaacctc tgtgccgtta aaagtcaaaa tggatagcgt cccggtgctg
     3901 acctatgtga atgattatgg tgcgcgtttg ccgctgttct tccgctgtac gggaaatacc
     3961 tgtcaggtgg acgaagagca aaacagcaaa ggccagggat aggagcaata gcatgaaagg
     4021 agcttattcg gcctgggcgt ggttattgct gtgtagcatt gtgagcacgc ctgtaatggc
     4081 ggggagtaaa tcggttgcga tggtcttgcg tgtgctcgtt gatgccccgc cgccctgtac
     4141 ggtaacgggt gcgagcgtgg agtttggtaa tgtcttcatt tccaagatcg atggggtaag
     4201 ttacaaacgc cccattgact actcgcttgt gtgcaacaat ctggcaatgg acgacctgcg
     4261 cttaaatatg caggcaacca caaccgtaat taacggagaa acggtaatcg atacgggtat
     4321 tgccggtttt ggtatccgta ttcagaaagt aagcgatcac tccattttgg atttaacgcc
     4381 tggcgcatgg ttaccgttta acttttcatc tggcgcactg gctctggaag cggttcctgt
     4441 cgtgcagagc ggtgtatcgc taacggcggc ggaatttagc gccagtgcca caattgtggt
     4501 ggattatcaa tgaaacggat ctctctctta atgttgtgga gctttagctt tatggcgctc
     4561 tcgaatgtct cttttcatgg ttatttagtg cagccaccca attgctctat tagtaatggg
     4621 caaaatatag agttgacgtt tcgggacgtg aatattgatg acattaacgg tagcaattac
     4681 gaacaggtcg tgcc
//
LOCUS       DBFIFS010000276         4682 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.276, whole genome shotgun sequence.
ACCESSION   DBFIFS010000276 DBFIFS010000000
VERSION     DBFIFS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.276"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..794
                     /locus_tag="N7A07_004399"
     CDS             <1..794
                     /locus_tag="N7A07_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2478933.1"
                     /translation="RQVSALEEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLER
                     DTNTIMTWQPTVQVIELAVNPTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNR
                     EYDAVIMRENFCAPWAEVEYLFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQST
                     RITGWEEWFALSGVSSPLVNNGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDM
                     VIPCDVPIRTGNRFIMTWQEEKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(828..1430)
                     /gene="cobO"
                     /locus_tag="N7A07_004400"
     CDS             complement(828..1430)
                     /gene="cobO"
                     /locus_tag="N7A07_004400"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU2478934.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1440..3092)
                     /locus_tag="N7A07_004401"
     CDS             complement(1440..3092)
                     /locus_tag="N7A07_004401"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2478935.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3200..4480)
                     /locus_tag="N7A07_004402"
     CDS             complement(3200..4480)
                     /locus_tag="N7A07_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGU2478936.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
BASE COUNT         1172 a         1133 c         1121 g         1256 t
ORIGIN      
        1 tcagacaagt aagtgcgctg gaggaatttt tacgtactcc gttattttca cactctaaaa
       61 aacgcatttt tcttaatgat acagggaagt attacctggg gatagtaaaa gaaacattaa
      121 ataagctgga acgcgatacc aataccatta tgacctggca gcctacggtt caggttattg
      181 aacttgccgt taatcctact tttagtaccc actggttaat cccaaattta catgaattca
      241 caaagttaca tcccgatatt attgttaata ttcattcttt agctaataac ggcgattttc
      301 ttaatcggga gtacgatgct gtcattatgc gcgaaaattt ttgcgcgccc tgggcggaag
      361 tagaatatct ctttgaagag gagattttgc cggtatgcag cggcagtttg cttgcaatgt
      421 ccgatcaaaa actgtcagtg gcagaactgc ttactgagtt accacttctg catcaaagca
      481 cccgtattac tggctgggaa gagtggtttg ctttatctgg cgttagcagt ccgctggtca
      541 acaacggacc acgtttcgat ttgctttcaa tgctgattgc cgccgtgcgc tctaatctcg
      601 gcgtggcgct gttaccacgt ttcgctattc aacatgattt ggatagtggc gatatggtga
      661 ttccctgtga tgtgcctatt cgtaccggta atcgttttat tatgacgtgg caggaagaga
      721 agtctgactc accgcacttg cagcaatttc gtgaatggtt gctggcgaaa tcggtcgtac
      781 cgcaggagat gtaaaacctg cgcctggcgg gatgccaggc gcagttatta ccagtcgata
      841 cccggctggg cctgaatgcc gttatcaaaa gcatgtttga cggggcggat ttcgctgacc
      901 gtatctgcca tttttaatat ctgactgtgg caaccgcgtc cggtgacaat gacactttgt
      961 tgtgccgggc ggttttgcag agaagcgatc acttcttcgg tatccagata atggtaagcc
     1021 agcatatagg tcagctcatc aagcaccact aagtcgtaac gcttgtcagc cagcatccgt
     1081 ttgctttccg accagacctc tttcgccgcg tcgatatccg cttgccggtt ctgtgtctcc
     1141 caggtaaaac cggttcccat aatatgaaac tcaaccccca gcggctggag aaggttgtat
     1201 tcgccgttgt cccattgccc cttgatgtac tgcgcaaccc ccaccgtttt gccatgaccc
     1261 accgcgcgcg tcacagtacc aaacgcggca gtggatttcc ctttgccgtt gccggtgaaa
     1321 acaatcaaga tccctttttt ctctatcgcc gccgcgacgc gcgtatcgac ctgttccttc
     1381 aatttctgtt ggcgctgcac atgccgttca gttgaaattc gcgcttccat tcattttcct
     1441 taatgtcccg cagggcaact ggcgcagcgg tgttgctgct caaactgact aaagaaatta
     1501 ttgcctttat catctaccag gatgaatgcg ggcagatttt ccacttccat catccacacc
     1561 gcttccatac ccagctccgg gtattccagg caacgcagac ttttgacata ttcttgcgcc
     1621 agtaacgcag ctgcaccgcc aatacttccc agattgaagc cgccatgttt gtgacaggca
     1681 tcggtgacct gctggctgcg gttacctttc gataacatca ctagactgcc gccagccgcc
     1741 tggaaggtat cgatataacc gtccattcga ccgcccgtgg ttggacccag tgaaccgcag
     1801 gccatgttct ccggtgtttt cgccgggcca gcgtaataga caatatgatg cttcaggtac
     1861 tccggcatcg gttcgccgct atccagccgc gcttttattt tggcgtgggc gatatcgcgc
     1921 gccactacga tgggaccact taacgacacg cgggtgccta ctggtaatct ggcgagatcc
     1981 tgcatcacgt cacgcaacgg acggtttaga tccagttgta catgttgcgc gtgattttct
     2041 tcccgcagtg aggctggaat atattgcccc ggattgtgtt cgagtttttc cagccagata
     2101 ccgtgtttgt taatttttgc tttgatattg cgatcggcag aacaggagag cgccatagct
     2161 atcgggcaag agccaccgtg acgcggcaga cgaatgacgc gaatatcatg agcgaaatat
     2221 ttacccccaa attgcgcacc aataccaaat tgctgactgg cctcgagcaa cactttttcc
     2281 agctcaatat cacggaatgc ttgtccctgc tcgtttccgc tggtgggcaa attgtcgtaa
     2341 tatttcgttg aggccagctt ggcaattttt aaggtttgat cggctgaaag ccctccgacg
     2401 acaaaggcga tgtggtaagg cgggcaggca gcggtgccca gcgatttcat tttttcgatt
     2461 aaaaatgcgg tcagtttttc tggttgcaat aaggatttgg tttcctgata aagcgcggct
     2521 ttattggcgg agccgccccc tttattaaca cagagaaaat ggtactcatc accagcgaca
     2581 gcgctgatat caatctgcgc cgggagattg gtttgcgtat tgacttcggt atacatatcc
     2641 agcggcgcat tttgtgaaaa gcgcagatta ttttcctgga aagtactgta aatgccttta
     2701 cttaaggctt cggcgtcgtt acctccggtc catatctgtt gccctttact ggcaacaata
     2761 gttgcggtgc cggtatcctg acagttgggt aatacccctt ttgctgagac ttcagcatta
     2821 cgtaataact gcaacgcaac gtatttatca ttactgctgg cctgctgatc gttaagaatg
     2881 ctggcgacct gttgcaaatg agcggaacgt aagaaaaaag aggcttcata aaatgcctgc
     2941 cgggcaagca gcgttaatgc ttcaggagcg atttttatca cttcttcgcc gtctaattcc
     3001 gttactgtaa tatgttgatc gctaattaat gtatattctg taccgtcttt attttgtaga
     3061 aacggttctt gccagataaa tggtttggac atatttcact tttcacctta aacgttagtg
     3121 ttacttaatt gccgtctaac ttcatgatcc ggcttataaa aatgcgtagt cattggcttc
     3181 tgattgctta agatccccct cagggcagag caacgttcga ttcattgggt tttaatgctt
     3241 taccccgaca gccctcgccg aaatgttcgc cttcccaccg actcaccatc gcagcgccca
     3301 ttgcgttgct catgacgttt gtcgcggaac gccccatatc aaggaacgga tcaacgccca
     3361 tcagcaagat cagccccgct tccggaatat tgaactggtt gagcgtggcg gcgataacca
     3421 ccattgaggc gcgtggtacg ccagccattc ctttcgaggt caacatcagg atcaacagca
     3481 tggtgatttg ctcaccgatg gataaatgga tattgcaggc ctgggcgatg aaaactgtgg
     3541 cgaaggagca gtaggccatt gatccaacga gattaaatga gtagccaatg ggtaagacaa
     3601 agctggcaat tttgggggaa acgccaaatt gctccagttt ttcaagcgtt cccggaaaag
     3661 ccgcttcaga actggatgtg gtaaatgcca gcagggcggg ttccgaaagg gcgcgggtca
     3721 ggcgtctgat gcaggggccg acataaacga tggccagacc gataagcagt acccaaagta
     3781 ataacatggt gaaataaaat tcacccataa agatcccggc gctcaccata actgccagtc
     3841 ctcgttcagc aatcaatgct gaaatagcag cgaatacggt caggggagcg aagagcatga
     3901 cgtagccagt gagctttaac atggcatgtg ccagcgaatc taaggcgtga acgatggcgc
     3961 tgcctttctc gccaatcgcc gtcaggctac agccgaggaa aattgagaac accacgattt
     4021 gcaaaatttc attgtgcgcc atggcatcga caatgctggt ggggaaagca tgcgaaataa
     4081 atacttttag cgtaaagggt tctgccgcga ccactccggt ggtttcggca ccgtgtgcaa
     4141 caaaattaat tcctgtacct ggcatgaaga aatttaccgt tatcaagcct aaggcaattg
     4201 acagcaatga ggcgcaaata aataaaaaga gtgttttaga aaaaatacga ccaagggctt
     4261 tggcatcgcc cattttagca atacctacca ccagggtaga gaccactaac ggagcgataa
     4321 tcatttttat cagtcgtaag aaaatcgtcg tgaatatcga tatttcttgc gcatagagtt
     4381 ttgcggtttc cggtgaagca gtgttattga gcactacgcc gataatcatt ccaagtacca
     4441 gcgccaaaag aatcatcgtg gttaaactta ttttcttcat tttttcctcc atcaggtgca
     4501 cggcgataaa tcagaatcag gagaagtaga tgcctgaata gagattcagg catctacctg
     4561 ctattacaac cttacaaata gctgaagtgt tgaactgcaa taagtgacgg gatgtcagtt
     4621 ggctaaaagt attcggattt ttacaaatcg aaactcatat ttaataaagg tgcatgttgc
     4681 tg
//
LOCUS       DBFIFS010000277         4666 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.277, whole genome shotgun sequence.
ACCESSION   DBFIFS010000277 DBFIFS010000000
VERSION     DBFIFS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.277"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            36..2417
                     /locus_tag="N7A07_004403"
     CDS             36..2417
                     /locus_tag="N7A07_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HGU2478937.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
     gene            2414..3043
                     /locus_tag="N7A07_004404"
     CDS             2414..3043
                     /locus_tag="N7A07_004404"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HGU2478938.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            3036..3857
                     /locus_tag="N7A07_004405"
     CDS             3036..3857
                     /locus_tag="N7A07_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HGU2478939.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            3857..>4666
                     /locus_tag="N7A07_004406"
     CDS             3857..>4666
                     /locus_tag="N7A07_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU2478940.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQN"
BASE COUNT         1151 a         1072 c         1225 g         1218 t
ORIGIN      
        1 gtgaatattt attttttcat tttcgggaac gcaagatgga tacgccagac gaaaaggaac
       61 gcattacatt tccgcaagta tcacgacgta gttttttaca agcgacttcc gcgcttatca
      121 ctttgccttt tatttcttct accgcaaagg cgcaatcccc agatgcgtcg ccagaagtta
      181 cagctccagt agctgataaa gtcgtgccca cctgtagcac atttgactgc ggtggtaagt
      241 gcgatattcg ggcgcatatg cgcgacggcg tggtgacgca aattaccacg cttcccgata
      301 acgaattgga tccacaaatg ccgattatgc gcgcctgtgt tcgtggtcgc ggctatcgta
      361 aatttgttta tcatccagac cgtttgaaat atccgatgaa gcgggtaggt aaacgaggcg
      421 agggtaaatt tgagcgtatc tcatgggatg aggcgacgac cttaattgcc gataacctga
      481 aaaggatcac gcaacaatat ggcccagctt cacgttatgt ccatgtcggg acggctgttt
      541 ctggtggaac attttctggc gatgcgatgg cgcgtcggct gttaaattta accggtggtt
      601 atcttgaata ttatcactca gttagtctgg gtaatactgc ggcggccacg ccttacacgt
      661 atggcgtggc cgcgagcggt aactcgatgg atactcttct ggataccaaa ctggtgattc
      721 tctgggggca taacccaacg gaaaccatat tcggtcacac caattactat tttcaaaaaa
      781 tgaagcaaaa cggtacacgt tttatcgtgg tggatccgcg ctattccgac accgtatctt
      841 cacttgccga tcagtggatc ccgctgttgc cgaccacgga caatgcgttg atggacgcga
      901 tgatgtacgt cattatcagc gagaatctcc acgataagac atttatcgac acatatactc
      961 tcggttttga tgaaaactcc atgccggaag gcgtaccagc caacgaatcg ttggttgcct
     1021 acctttttgg cgcaaaagat gggatacaca aaacgccgga atgggcagaa aaaattaccc
     1081 acgttccagc acaatccatt cgtcaactgg cacgcgatta cgccactaca aaacccgcag
     1141 cgttgattca gggatggggg ccacagcgac atatttgcgg cgaacgcacc gcccgaggtt
     1201 caactttact ggcctcgatt accggtaacg tcggcatcaa aggtggctgg gcggcaggtt
     1261 acggcggtag ttctaatcgt aaattctgcg ttggaccaga tatgccagaa aatccggtac
     1321 aagcgaaaat ctcgattatg aactggatgc aggcggcgga tgatgcttct aaagttacgc
     1381 cgcaagatgg gcttaaaggt gtcgataaac tcgacagtaa tatccgcctg ctgttctcac
     1441 tggcaggtaa ttatctggcg aaccagaacc cggacgtcca ccaggccgcg aagctccttg
     1501 aagacgaatc taaaatagaa ttcatcgtac tcagcgatct gttcatgact ccgagcgcga
     1561 aatatgccga tgtgttgctg ccggaaacca gctttatgga acgctggaat atcggcgaaa
     1621 catggggaac tgcgagctat cttattctgt cagaaaaacc gattgagccg gattttgaac
     1681 gtcgcaccga ttatgactgg ttgcgtgacg tggcgaaaaa actgggtgtc gaagccgagt
     1741 tcagtcaagg acgtgatgag aagcaatgga tcgagcatat ctgggagcaa actcgtctgg
     1801 cgatgccgga tgaaaatttg ccggacttcg caaccctgca aaaaacacgt cgacatcttt
     1861 tcaaaagcgc gccgcacatc gctttcgaag caaatattcg cgatccgcag aataatcctt
     1921 tcccgacgcc ttccggcaaa attgagatct tctctaagcg tctttttgat atgcaagatc
     1981 cagaaattcc cgcgctttcg cactatgtcc ctgcctttga agggccagaa gataaactga
     2041 cggcgaaata cccgttgcag ctcatcacct ggaaagggaa aaaccgtgct aattccaccc
     2101 agtatgctaa cccgtggctg caagaagttc agacgcaaaa actctggctg aatccgcaag
     2161 atgcaaaaca gcgcgggatt agcgaaggag atagcgtaaa aatttataac gatcgtggtg
     2221 tgagcattat cccggtggaa ataacccccc gtatcatccc tggcgtagtt gctatgcagg
     2281 cgggggcatg gtggcaaccg gatgcacaag gtatcgatcg cggaggctgt gccaatgtac
     2341 tgagttcgac ccgcattacg gcgctggcga aagggaattc gcatcaaaca atgctggtgg
     2401 aggttgagaa agcatgagcc aatttaaaga atttgcatct gtcagcgaca aacaattagg
     2461 gttctttatt gactcttcgc gttgttcggg ctgtaaagcg tgtcaggtgg cctgtaaaga
     2521 caaaaacaac cttgaggttg ggcgacgttt tcgccgcgtg tatgaagtga acggaggtgg
     2581 cttcatcgcc actggagagg gcggtgtgag tcataatgtc tttgcttata ccctttctgt
     2641 ttcctgtaac cattgcgcgg atccgatctg taccaaaaat tgcccgacga tggcgatgca
     2701 taagcgtccc ggagacggta ttgtgcgcgt gaatactgat aagtgcgtcg gttgcggtta
     2761 ctgcgcctgg tcctgtcctt acggcgcacc acaaatgaat gagcagaccg ggcagatgtc
     2821 gaaatgcgat ttctgtattg atctgcaagc taaaggagaa caacccattt gcgtggcaac
     2881 gtgcccgttg ggggcgatta agttcggccc tattgatgag ttgcgtgaaa agtatggcga
     2941 tgtcagtgat gtgaaaggtt tacctgattc gtcgattacg cagccaaatc tggtgattaa
     3001 accgcatcag ggcgcggaaa aggaggcgtc acatcatgca tgaattaccg ttaatggctt
     3061 ttacgctgtt gttacaggca tcggtcggta tgacgttgtg gctggcgttt cttcatcgtc
     3121 atattttaat cacctccagc gcccgcccgg ttatgcggcc gccattactg ttggcttttt
     3181 tggcaggtgc cgtagggtta ctgatttcta ccttacacct gggttatcca cttaatgcga
     3241 tgcatgcgct gagccacttt tccagttcat ggctgagtcg ggaaattatc tttggtgcgc
     3301 tgtatctggc gctattgggt ttatccacat tactggtcat gttacgcaaa ggcggctggc
     3361 aactcctgct tatgctagcg gcagtggtcg ggattgttga tgttttctgc atggcgcaag
     3421 tttacatcca tacgtcgatt atcacctggc agcattacaa cacgctgatt atgttcttag
     3481 gtacagtggg aattctcggt tcagcgctgg tagcagtgtt tcgtattagc ggaattttgc
     3541 cgcagattga tgccctgcgt aatggatgcg tgttggtgat cgctctcctg gttctgctgc
     3601 gtttgttggt tcagccgttg tggatagggg atttaacggc aaatgcgatg cagatagcca
     3661 cattgccaca tgcgccatta gcgatgctgg gacagttaaa acccatactg acactaagct
     3721 ggggaatttc cgtcattggg atgatgttct ttgctgtcgg tggttgtaaa aagaatatcc
     3781 cggcggcgtt attcggtagc gtaatgctag tggggagtga agttatgctg cgttttgtct
     3841 tttttagtat cggctaatgt taagtcataa cataacgatt ggcgcggaat gcgcgcgcca
     3901 acgctttcgt tttgcaacat gtcatgcctg tgtcgaggct tgcccggcac aggcgctaac
     3961 gctgacagaa gagggcatag cggtcgacgc ggaacaatgt attgattgtg cggtttgcca
     4021 gtttatttgt ccgcaagagg ctattcgtgg cgttaattcc cctttacgtt atttttgcaa
     4081 tcacaccctg gtcgcacctt tcaccactat tgcgccgacg ctggaagaac ttttgctatg
     4141 gcataaagag caggggatca cagcgatcgc gattgatctg agtcaggcga aggcatggga
     4201 gcgcgcatta ttgctgttaa acgaatgctt acggcaatat ggcgaggaag gctggtctgt
     4261 ggtatctcca cataccagtg aggtgaatgt ttcccggcgt cgtttgttcc atgtgcagcg
     4321 tgcggatgtt cagatgacac aaatacaacc aggattacgt cggttacatt atttatggcc
     4381 ccagattagt gatgctcgcc cgatgatgga tcctcaaaag tgtcagcttt gcggtgcatg
     4441 ctggcgggcc tgcgagcaac aggtcttttc gcttaacgaa ggccatttac aaataaatga
     4501 tgccttgtgt aacggatgcc aaaattgtat tgctgtttgt tttcatcagg caatgaccgt
     4561 tgaattgacg attctaccgg caaaaattgt gaatcttcat gctaatcgca aagtctgtaa
     4621 aacctgtcaa aaatcctttc ttacttttca gcaaaatgcg caaaat
//
LOCUS       DBFIFS010000278         4625 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.278, whole genome shotgun sequence.
ACCESSION   DBFIFS010000278 DBFIFS010000000
VERSION     DBFIFS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.278"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..617)
                     /locus_tag="N7A07_004407"
     CDS             complement(<1..617)
                     /locus_tag="N7A07_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU2478941.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(732..807)
                     /locus_tag="N7A07_004408"
     tRNA            complement(732..807)
                     /locus_tag="N7A07_004408"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Thr,seq:ggt)
     gene            complement(814..888)
                     /locus_tag="N7A07_004409"
     tRNA            complement(814..888)
                     /locus_tag="N7A07_004409"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Gly,seq:tcc)
     gene            complement(1005..1089)
                     /locus_tag="N7A07_004410"
     tRNA            complement(1005..1089)
                     /locus_tag="N7A07_004410"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Tyr,seq:gta)
     gene            complement(1098..1173)
                     /locus_tag="N7A07_004411"
     tRNA            complement(1098..1173)
                     /locus_tag="N7A07_004411"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Thr,seq:tgt)
     gene            1535..2485
                     /gene="coaA"
                     /locus_tag="N7A07_004412"
     CDS             1535..2485
                     /gene="coaA"
                     /locus_tag="N7A07_004412"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU2478942.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2514..3479)
                     /gene="birA"
                     /locus_tag="N7A07_004413"
     CDS             complement(2514..3479)
                     /gene="birA"
                     /locus_tag="N7A07_004413"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU2478943.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3476..4504)
                     /gene="murB"
                     /locus_tag="N7A07_004414"
     CDS             complement(3476..4504)
                     /gene="murB"
                     /locus_tag="N7A07_004414"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU2478944.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1169 a         1167 c         1057 g         1232 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaggaatcc aagaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcgcgagcag caccgccgta ggttttagcc agtacggtag tgattgcagc ggtcagcgtt
      541 gttttaccat ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca
      601 aacttttctt tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac
      661 atcaaccagg caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag
      721 gagaaaagaa gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt
      781 gcgctctacc aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg
      841 catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc
      901 ggctacctga ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt
      961 aagcgaactt atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg
     1021 attcgaacct tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg
     1081 aaccccaccg gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca
     1141 agtcagttgc tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac
     1201 atgcgagttc atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga
     1261 cacgatgaag aaacagccga atggagggca gattagcaca ctttttcaac atcattgtgc
     1321 tcaacaatgc gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt
     1381 gcttttcaac gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt
     1441 taccctcctg cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt
     1501 tgccgataga gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat
     1561 gacgccttac ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat
     1621 gacgttatcg gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga
     1681 agaagttgcc gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa
     1741 tctgcgccgt caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta
     1801 cattatcagt attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca
     1861 ggcactatta agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt
     1921 ccttcaccct aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga
     1981 atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa
     2041 cgttacagcc cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac
     2101 tgttgttcag cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga
     2161 ttatccacac gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt
     2221 tgatgcaccg gaagacttac ttcagacatg gtatatcaac cgttttctga aattccgcga
     2281 aggggctttt acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga
     2341 agctattaag actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa
     2401 tattctacct actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt
     2461 agaagaggtc agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt
     2521 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     2581 agcaataaag cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     2641 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa
     2701 ggtgccaatc cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg
     2761 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     2821 ttaacgacac tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt
     2881 tgcgccgcat cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc
     2941 tgcagataga ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat
     3001 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     3061 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     3121 ccccggcgac cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc
     3181 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacagttaca
     3241 ctaccgccat ccagctgacc caatatctgt tcagcattaa gtaactggat aggctcaggc
     3301 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     3361 atgtgtttat taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag
     3421 tgaaattcgc cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg
     3481 aaattgtctc cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa
     3541 gccagacatt aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat
     3601 cttcgctttt tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcaa
     3661 ccccacccat ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt
     3721 ttactgaacc atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt
     3781 tagccgtttc ggcagataca acagggtttt tgaagaaact accggcattg ccattcactt
     3841 ttggatcagg gagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg
     3901 ttactgttgt aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt
     3961 ttggcagacg cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa
     4021 tactgtcgcg atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg
     4081 ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa
     4141 tattctggat aggtgatgag ccgacacaac caggaattaa tgccagattt tccagaccag
     4201 gcataccttc ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat
     4261 gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca
     4321 ccgtgccgcg atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg
     4381 gttgcccttc tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac
     4441 atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt
     4501 tcatagctgc tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc
     4561 ttgtagttat gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg
     4621 ggcct
//
LOCUS       DBFIFS010000279         4597 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.279, whole genome shotgun sequence.
ACCESSION   DBFIFS010000279 DBFIFS010000000
VERSION     DBFIFS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.279"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..700
                     /locus_tag="N7A07_004415"
     CDS             <1..700
                     /locus_tag="N7A07_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2478945.1"
                     /translation="HLIKLFQTYGFYIHGEKETHNGKEFVYARSLHEPYGDILLDYPR
                     IMTSRANKYLLAIYPEYHTRLFPDSKLVNESPDIVKDISHANSIHKIYICGMRSVMGM
                     KRGDIIVIYRTGDKKGPARYRSVASTLCVVESVKNISEFLSEDSFVDYCIRFSVFSED
                     ELRKIYKERRYPFIIRFTYNLSLPKRPNRAILIDHVGLNGSRAFRWSHFKLTNEQFLK
                     IIELGKINESFIIH"
     gene            678..1055
                     /locus_tag="N7A07_004416"
     CDS             678..1055
                     /locus_tag="N7A07_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU2478946.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            1536..1742
                     /locus_tag="N7A07_004417"
     CDS             1536..1742
                     /locus_tag="N7A07_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU2478947.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1818..2114
                     /locus_tag="N7A07_004418"
     CDS             1818..2114
                     /locus_tag="N7A07_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU2478948.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2120..2905
                     /gene="bet"
                     /locus_tag="N7A07_004419"
     CDS             2120..2905
                     /gene="bet"
                     /locus_tag="N7A07_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2478949.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2902..3579
                     /locus_tag="N7A07_004420"
     CDS             2902..3579
                     /locus_tag="N7A07_004420"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2478950.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            3579..3761
                     /locus_tag="N7A07_004421"
     CDS             3579..3761
                     /locus_tag="N7A07_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2478951.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            3734..3925
                     /locus_tag="N7A07_004422"
     CDS             3734..3925
                     /locus_tag="N7A07_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2478952.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            4002..4217
                     /locus_tag="N7A07_004423"
     CDS             4002..4217
                     /locus_tag="N7A07_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2478953.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4316..4537
                     /locus_tag="N7A07_004424"
     CDS             4316..4537
                     /locus_tag="N7A07_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2478954.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            4534..>4597
                     /locus_tag="N7A07_004425"
     CDS             4534..>4597
                     /locus_tag="N7A07_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2478955.1"
                     /translation="MSEINYQALREKAEKATKGSY"
BASE COUNT         1365 a         1054 c         1075 g         1103 t
ORIGIN      
        1 ccatctaata aaacttttcc aaacgtacgg attttacatt catggtgaaa aagaaacaca
       61 taacgggaaa gagtttgtat atgcgaggtc tttgcatgag ccttatggtg atattttatt
      121 agattaccct cgaataatga catcaagagc caacaaatat ttactggcga tttatcccga
      181 atatcacact agactattcc ctgattcaaa acttgtaaat gaatcaccag atattgtcaa
      241 agatatatcc catgctaaca gcattcataa aatttacata tgtggaatgc gttctgtgat
      301 gggaatgaaa agaggagata tcattgtcat ctatagaacc ggagacaaaa aagggccagc
      361 tcgctatcgt tctgtagcca gtacattatg tgtagttgag agcgtaaaaa atatttctga
      421 atttttaagc gaagatagtt ttgtagacta ttgtattcgt tttagcgtat tttctgaaga
      481 tgaactcaga aaaatctata aagaacgtcg ataccctttc attataagat tcacatacaa
      541 tctgtctttg ccaaagagac ccaatcgtgc tattttaata gatcatgtgg ggctaaatgg
      601 ttcgcgtgca ttccgatgga gtcactttaa actcacaaat gagcagttct taaagatcat
      661 cgagttaggc aagataaatg aaagttttat tatccattaa gcctgagttt gcagaaaaaa
      721 tattgaacgg aacaaagcgg ttcgagtttc gtaaaggtat attcaaaaat ccgcaaatta
      781 gcaccgttgt tatttatgcc acgatgccat taggtaaagt tgttggtcaa ttccgtattg
      841 aatcaatact aagtgacgaa ccggaatctc tttggaaaaa gacggaaaaa cacgcaggta
      901 tttctaagca attttatgac tcatattatt caggtagaga aaaggcctac gcaataaaaa
      961 ttggtgaagt ggaaagatat aaagaaccaa ttcctatctc tgctctaggt agtaatatta
     1021 agccaccaca atcatatctt tacctacctg cgtaagaatc ccggccaccg tgccgggttt
     1081 tcttttgccc ctccccctca tcacacaaac cattcaaaaa accaccacga cctcgcttca
     1141 gttatcgcta tgcgatgcaa gtcacaaaat aaatccatcc taaatacaac catttacatc
     1201 taaaaaaacc aataaaacaa cttttgttgt tgacgacaaa acaactatag ttttaaataa
     1261 attcatcgca acaacacaac gatacggcaa ccacctgatt caccgttgcg atgaccgctt
     1321 agatccgcag cttgaatttc agcaggctcc gggaagtgcg aggggtgaag tggacgcgtg
     1381 aacgtcggtg tgaccagctg aaatcaactc aacacctcat acctcagtcg cttcaacgag
     1441 gcggcttagt tatgacaacc ggcggccatc caccgcctga atacgcgcag aagtctctat
     1501 atgttcagca gcccagctta cgggcaggag tttttatggt tcatcaacat tacggaacgc
     1561 agaccgttaa tcgcggtgcg gtcatgccag gaatgctggt caaacacaaa gatggtacct
     1621 ggactgcatc agctaattta cgcggacggc tttatctgca tcgcggcatc gagcgcactt
     1681 atacccgtga tttgctcgtg gaagtttttc tcgacggacg cggcaacggc ctgaatcact
     1741 aatccccttt cctgtttacc taatcagccc ggcatttcgc gggcgatata ttcacagcca
     1801 ttttcaggag ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcagagct
     1861 gggcgcgtca ttaccagcag attgcccgtg aagagaaaga ggcagaactg gcagacgaca
     1921 tggaaaaagg tcttccacag cacctgtttg aatcactctg catcgatcat ttgcaacgcc
     1981 acggggccag caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc
     2041 gcatggcaga acacatccgg tacatggttg aaaccattgc tcaccaccag gttgatattg
     2101 attcagaggt ataaaacgga tgagtacagc actcgcaacg ctggcaggga agctggctga
     2161 acgtgtcggc atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc
     2221 atttaaaggt gatgccagcg atgcgcagtt catcgcattg ttgatcgtcg ccaaccagta
     2281 cggccttaat ccgtggacga aagaaattta cgccttccct gataagcaga acggcatcgt
     2341 tccggtggtg ggcgttgatg gctggtcccg catcatcaac gaaaaccagc agtttgatgg
     2401 catggacttt gagcaggaca atgaatcctg cacatgccgg atttaccgca aggaccgtaa
     2461 tcatccgatc tgcgttaccg agtggatgga tgaatgccgc cgcgaaccat tcaaaacccg
     2521 cgaaggcaga gaaatcacgg ggccgtggca gtcgcatccc aaacggatgt tacgtcataa
     2581 agccatgatt cagtgtgccc gtctcgcctt cggatttgct ggtatctatg acaaggatga
     2641 agccgagcgc attgtcgaaa ataccgcata cactgcagaa cgtcagccgg aacgcgacat
     2701 cactccggtt aacgatgaaa ccatgcagga aattaacact ctgctgatcg ccctggataa
     2761 aacatgggat gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgtgc
     2821 atcgtcagaa ctgacacagg ccgaagcagt aaaagctctt ggattcctga aacaaaaagc
     2881 cactgaacag aaggtggcag catgacaccg gacattatcc tgcagcgtac tgggatcgac
     2941 gtgagagctg tcgaacaggg agatgatgcg tggcacaaat tacggctcgg cgtcatcaca
     3001 gcttcagaaa ttcacaacgt aatagcaaaa ccccgatcag gaaagaagtg gcctgacatg
     3061 aaaatgtcct acttccacac cctgctggct gaggtttgca ccggtgtggc tccggaagtt
     3121 aacgctaagg ctctggcctg gggaaaacag tacgagaacg acgccagaac cctctttgag
     3181 ttcacttccg gcgtgaatgt tactgaatcc ccgatcatct atcgcgacga aagtatgcgc
     3241 accgcctgct ctcccgatgg tttatgcagt gacggcaacg gccttgagct gaaatgcccg
     3301 tttacctccc gggatttcat gaagttccgg ctcggtggtt tcgaggccat aaaatcggct
     3361 tacatggccc aggtgcagta cagcatgtgg gtgacacgaa aagatgcctg gtactttgcc
     3421 aactatgacc cgcgcatgaa gcgtgaaggc ctgcattatg tcgtgattga gcggaatgaa
     3481 aagtacatgg cgagttttga cgagatggtg ccggagttca tcgaaaaaat ggacgaggca
     3541 ctggctgaaa ttggttttgt atatggggag caatggtaat gaagcatcct cacgataata
     3601 tccgggtagg cacgatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg
     3661 gcctttctgt tatccgaaat ccactgaaag cacagcggct ggctgaagag ataaataata
     3721 aacgaggggc tgtatgcaca aagcatctcc tgttgaatta agaacgagta tcgggatggc
     3781 acatagcctc gctcaaattg gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga
     3841 atttcatacg ttagccacat ccctttcaca aaagctggaa atgatggcgg cgaaagcaga
     3901 agcaaacgag agagacccgg catgacaaca acagaatgca tttttctggc agcaggcttc
     3961 atattctgtg tgcttatgct tgccgacatg ggacttgttc aatgacacct cagcaggaaa
     4021 acgcccttcg cagcattgcc cgtcaggcta attctgaaat caaaaaagcc agacagcagt
     4081 ttccggataa aaacgtcgat gacatttgcc gtagcgtact gaagaagcac cgcgaaacgg
     4141 taacgctaat gggattcaca ccgactcatt taagtctggc gatcggcatg ttaaacggcg
     4201 tctttaagga gcgatgaaca tgaaaagcaa aatcatcagg gagctacagg ctcctttttt
     4261 attgttcgca ttcaccctca agcgtattaa ccaacaattc agggattaat ggaagatggc
     4321 agacatcatt gattcagcat cagaaattga agaattacag cgcaatacag caataaaaat
     4381 gcgtcgcctg aactaccaga ctgtatccgc aactcattgt tgtgagtgtg gcgatccgat
     4441 agatgaacga agacgcctgg cagttcaggg ttgtcggact tgtgcaagtt gccaggagga
     4501 tctggagctt atcagtaaac agagaggttc gaagtgagcg aaattaatta tcaggcactg
     4561 cgtgaaaagg cagagaaagc aactaaagga agctaca
//
LOCUS       DBFIFS010000280         4597 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.280, whole genome shotgun sequence.
ACCESSION   DBFIFS010000280 DBFIFS010000000
VERSION     DBFIFS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.280"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            216..1373
                     /gene="acrE"
                     /locus_tag="N7A07_004426"
     CDS             216..1373
                     /gene="acrE"
                     /locus_tag="N7A07_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU2478956.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1385..4534
                     /gene="acrF"
                     /locus_tag="N7A07_004427"
                     /pseudo
     CDS             1385..4534
                     /gene="acrF"
                     /locus_tag="N7A07_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1117 a         1118 c         1200 g         1162 t
ORIGIN      
        1 tttttaattc tgcctctttc aacccgcgtc aaaataaaac agtagaatat taatcttttt
       61 ttgtgtttat gtgccttgag acgcctgtat tcataactat tccttacatc gacgaatgat
      121 aatttgtagg atagcgaact gtatttttct ttctgcgagt taacgcgttg cctttttggg
      181 taaataacgc gcttttggtt ttttgaggaa tagtaatgac gaaacatgcc aggtttttcc
      241 tcctgccctc ctttattctg atctccgcgg ctttaatcgc cggttgtaac gataagggag
      301 aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca tattgtaaaa acggccccgt
      361 tagaagttaa gactgaatta ccaggccgca ccaatgctta tcgtatagcc gaagttcgcc
      421 cacaggttag cgggatcgta ctgaatcgca atttcactga aggcagcgat gtgcaagcag
      481 gccagtccct gtaccagatc gatcccgcga cctatcaggc aaattatgac agcgcgaaag
      541 gcgaactggc gaaaagtgaa gccgccgccg ccatcgcgca tttgacggta aaacgttacg
      601 ttccgctcgt gggtacgaaa tacatcagcc agcaggagta cgaccaggcc attgctgatg
      661 ctcgccaggc cgatgccgcc gtgattgccg caaaagccac agtcgaaagc gctcgcatca
      721 atcttgctta taccaaagtg actgcgccaa ttagcggacg tatcggcaaa tcgactgtga
      781 ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact ggcaactgtc cagcagctcg
      841 atcctatcta tgttgatgtg acccaatcca gcaacgattt tatgaggctg aagcagtccg
      901 tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa cgtagagttg gtcatggaaa
      961 acggtcaaac ctatcccctg aaaggtacgc tgcaattctc cgatgtgacc gttgatgaaa
     1021 gcaccggctc cataacccta cgtgctgtct tccctaaccc gcaacatacg cttttgccgg
     1081 gtatgtttgt gcgtgcacgg attgatgaag gcgtccaacc tgacgccatt ctcatcccgc
     1141 aacaaggcgt tagccgcaca ccgcgtggtg atgcaaccgt gctgattgtt aacgataaaa
     1201 gtcaggtgga agtgcgccct gtcgttgcca gtcaggcgat tggcgataaa tggttgatta
     1261 gcgaaggtct gaaatctggc gatcaagtca ttgtcagcgg cctgcaaaaa gcgcgtccgg
     1321 gagagcaggt taaagccact accgataccc ccgcagatac tgcatcgaag taaggtaatc
     1381 tgacatggca aactttttta ttcgacgacc gatatttgca tgggtgctgg ccattattct
     1441 gatgatggcg ggcgcactgg cgatcctaca attgcccgtc gctcagtatc caaccattgc
     1501 accgcctgcg gtttctgttt cagcaaacta tccgggcgct gatgcgcaga ccgtgcagga
     1561 tacggtgacg caggttatcg aacagaatat gaacggtatc gataacctga tgtatatgtc
     1621 ctccaccagc gattccgccg gtagcgtgac aattaccctt accttccagt ccgggaccga
     1681 tcctgatatc gcgcaagtgc aggtgcagaa caaactccag ctcgccacgc cgttgctgcc
     1741 gcaggaggtt cagcagcagg ggatcagtgt tgaaaagtcc agtagcagct atttgatggt
     1801 ggcaggcttt gtctctgata acccagacac cacacaggac gatatctcgg actatgtggc
     1861 ctctaacgtt aaagatacgc ttcgccgtct gaatagcgtc ggtgacgtac agcttttcgg
     1921 cgcacagtat gcgatgcgta tctggctgga cgccgatctg ctaaacaaat ataaactgac
     1981 accggttgat gtgattaacc agttgaaggt acagaacgct cagatcgctg ccggacagtt
     2041 gggcggaacg ccagcgttac cagggcaaca gttgaatgcc tcgattattg ctcagacgcg
     2101 gttaaaaaat ccggaagaat tcggcaaagt gaccctgcgc gtaaacagtg acggctcggt
     2161 ggtgcgtctg aaagatgtcg cacgggttga acttggcggt gaaaactata acgttatcgc
     2221 tcgcatcaac ggaaaaccgg cggcgggcct gggtattaag ctggcaaccg gcgcgaatgc
     2281 tctcgatacc gcgaaagcca ttaaggcaaa actggcggaa ttacagccat tcttcccgca
     2341 gggaatgaag gttctctacc cttatgacac cacgccattc gtccagcttt ctattcacga
     2401 agtggtaaaa acactgttcg aagctattat gctggtgttc ctggtgatgt atctgttctt
     2461 gcagaatatg cgagcaacgc tgatccccac cattgcggta cccgtggtgt tgttagggac
     2521 gtttgccatc ctcgccgctt ttggttactc catcaacaca ctaacgatgt tcgggatggt
     2581 gcttgccatc gggctgctcg tcgatgatgc gatagtggtg gtggagaacg tcgagcgcgt
     2641 gatgatggag gataagctcc cgccaaaaga agcgacggaa aaatcaatgt cgcaaataca
     2701 gggcgcactg gtgggtatcg cgatggtgct gtcagcggta tttattccga tggcattctt
     2761 cggcggttct actggggcaa tttatcgtca gttctctatc accatcgttt cggcaatggc
     2821 gctttctgtt ctggtggcat tgattcttac ccctgcgtta tgtgcaacac tgctcaaacc
     2881 cacctcagct gagcatcacg aaaataaggg tggtttcttc ggctggttta ataccacctt
     2941 cgatcatagc gttaaccact acaccaacag cgtcggcaaa atcctcggtt ccacaggacg
     3001 atatttactg atctatgcgc tgattgttgc aggaatggtg gtgttgtttt tacgtcttcc
     3061 gtcttccttc ttacctgaag aggatcaggg tgtctttctg accatgattc agttacccgc
     3121 tggcgcgacg caagagcgga cgcaaaaagt gttggatcaa gtgacggatt actatctgaa
     3181 gaacgagaaa gcaaacgttg aaagtatctt tacggttaac ggctttagct tcagcggcca
     3241 ggcacctccg caaccccatg ttttcacata actgttgcgt tgaccaattg aatctacagg
     3301 tatggccttc gtcagtctga aaccgtggga agagcgtaat ggtgatgaaa acagtgcgga
     3361 agcggtaatc catcgtgcca aaatggagtt gggcaagatc cgcgacggtt ttgtcattcc
     3421 attcaatatg ccagccattg ttgaactggg cacggcaacg ggtttcgact ttgagttaat
     3481 tgataaggct gggctgggtc acgatgccct aacccaggcc cgtaaccagt tgcttggtat
     3541 ggcggcgcaa catcctgcca gcttagtcag cgtgcgtccc aatggcctgg aagacaccgc
     3601 gcagtttaaa ctggaagttg accaggaaaa ggcgcaggca ttaggtgttt cactttctga
     3661 catcaatcag accatttcaa cggcgttggg tgggacttac gttaacgact tcatcgaccg
     3721 tggtcgcgtg aaaaaggtgt atgttcaggc ggatgccaaa ttccgtatgc tgccagaaga
     3781 tgtcgataaa ctttatgtcc gcagcaccaa cggcgaaatg gtgccattct ctgcctttac
     3841 cacttcacat tgggtgtatg gctctccgcg actggaacgc tacaacggtc tgccgtcaat
     3901 ggagattcag ggggaagccg cgccaggaac cagttccggc gatgccatgg cgttgatgga
     3961 aaacctagcg tcaaaattac ctgcgggcat tggttatgac tggacgggta tgtcgtatca
     4021 ggaacgctta tcgggaaacc aggctcccgc tctggtagca atttcctttg tggttgtttt
     4081 cctgtgcctt gctgcactct atgaaagctg gtcaattcct gtctcggtta tgttggtcgt
     4141 gccgttaggg attgtcggcg tgctgctggc ggcgacactc tttaatcaaa aaaatgacgt
     4201 ctactttatg gtaggcttgc taacgacaat tggcttgtcg gccaaaaacg ctattttgat
     4261 tgttgagttc gctaaagatc tcatggagaa agagggtaaa ggtgttgttg aagcgacact
     4321 aatggcagta cgtatgcgtc tgcgtcctat cctgatgacc tctctcgcct ttattctcgg
     4381 cgtattaccg ctggctatca gtaacggtgc cggcagtggc gcgcagaacg cagtgggtat
     4441 cggggtaatg ggaggaatgg tctctgcaac gttgctggca atcttcttcg taccggtgtt
     4501 ctttgtggtg atccgccgtt gctttaaagg ataaatctga aacataaagg cgctttcggg
     4561 tgcctttatg ttttccagtg aaacccataa aaattaa
//
LOCUS       DBFIFS010000281         4549 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.281, whole genome shotgun sequence.
ACCESSION   DBFIFS010000281 DBFIFS010000000
VERSION     DBFIFS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.281"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            322..921
                     /locus_tag="N7A07_004428"
     CDS             322..921
                     /locus_tag="N7A07_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2478957.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            918..1538
                     /locus_tag="N7A07_004429"
     CDS             918..1538
                     /locus_tag="N7A07_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478958.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            1535..1735
                     /locus_tag="N7A07_004430"
     CDS             1535..1735
                     /locus_tag="N7A07_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478959.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            2143..3120
                     /locus_tag="N7A07_004431"
     CDS             2143..3120
                     /locus_tag="N7A07_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU2478960.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3405..4145)
                     /locus_tag="N7A07_004432"
     CDS             complement(3405..4145)
                     /locus_tag="N7A07_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2478961.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4266..4496)
                     /locus_tag="N7A07_004433"
     CDS             complement(4266..4496)
                     /locus_tag="N7A07_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGU2478962.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1110 a         1194 c         1243 g         1002 t
ORIGIN      
        1 cgactttccg gaagtgtacc acctgagaaa atcatcatat cgctgttttc cgcccctgcg
       61 cggcgcatca cagtccagag atacaacaca acatatagtg ccatgggcaa ctgaatgagg
      121 cactgcatgc tgtgctgacg ggatgtaacg ctgtgcggag ggagaggaag atccgtaacc
      181 accagaaacc ggatctgacc aggaaaagag aaagagtgta tgtaagttct gtactgtatg
      241 tatgaattgt attgtatgta ttgtgtattt ttcataccgg gcatacgatg gcgccattcc
      301 ctgagaaacc ggagccagac catgacatcc ctctccccgg acaccgtccg ccgcattgaa
      361 gacgccgccg ccgccctgat cgccgccggc accccgaacc cgaccaacga gcaggtccgt
      421 cagcacctcg gcggcggctc cctgtcccac atctccccgg tgatgcgcgc gttccgcgcc
      481 cgccagcgcg agcaggccac gcccctgccg ccggagctgg cgcagctgct gaccggccag
      541 ctggggctgc tgtggcaggc ggccgtgaaa caggctgaag ccggggccct ggcggcgcgc
      601 gagcaggccg acgacgacat tgcccgggcc gacaaggagc gggatgaggc gctggcgaac
      661 gtggccgcgc tggaaagcga gctggcggtg ctgcgcgagg tggtggccga acgtgaccgc
      721 ctgctgcagg aggtacgcga gctacgcgcg gaggcgctgc cgctgcgcga gcaggtggcc
      781 cgcctgaccg ccaccggcga acacctggcg gcgcagctgc aggacacgaa ggcggagctg
      841 aaggaagccc gggaggacgg ccgccagctt cagaccgaac tgctggctct ggcccgccag
      901 gacgggaagg tgaagaaatg agacaggaaa gagtattacg tataaaggca tttcgacggg
      961 cactggagct tggtggcggt gaacgggctg acgggtgtcg tcattttccc cgctggaaaa
     1021 cggaactgag gaatttcccg cacagcagct gcgatttagc cagcaatact ctggcgtact
     1081 acctcaaaga acgagagaga tgccatccgt gcatcatttt tatggaaggg aacgctggct
     1141 ttcatgaagc ggaaaactca acagttcacg ggcatgtcat cgtattgctg gacggagagt
     1201 acatcgattt gacgctggat caattcgacg aatatccgga ctacattcct tacgaacctg
     1261 tcgaatctga cgggcagatt ggtaagctgt tgcgtaatat catgaagcat gaggacccgg
     1321 taaaaacccg ccagattgat ctcgatggtg gcgaagagct gtatgcctgg ttacgcgata
     1381 ccgccgacga agtgctggca gcagatccgg aatggcaggc ccgggaacgg tcgatagaag
     1441 aagcaaggga agccgctatt acggtgttcc cgtttctgtc tgacgtgcaa aaaactgagt
     1501 gcgaacaggg cccggacagt caggaggctg ccagatgaag atttatcatt acactgacct
     1561 gaacggccta aagggcatca tcgaatcagg ctcactgtgg gcgacgcact tttcctttct
     1621 caatgacagt aatgagctga ctcacggcat gaactgcctt gagaatgccc tgcagtacct
     1681 gagggacgac tttaacccga aaacccttaa gttcatcgaa caggcaccct tataagcggt
     1741 tgtgtatgct gtttccctgg aatgatttat ccacatatcc ggaggctcga tccagtaaac
     1801 agatccatga atgatcaaca aaggatccat taaagatccc cataccgctg caaaccttgt
     1861 cactcatggg ccgggaccac gatcacataa gcagtggcat gttactgata aactgtaaca
     1921 tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg acataaacta
     1981 tggtcagtac gccagactca gctgttaaat acaggctgca ggtttttctt cagtcagtta
     2041 gcggggctct gacacacgat ttgctgttta ttcttttact gtccacaggc aggaggcttt
     2101 ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa gtcgtccggt
     2161 gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc gctgatgcgt
     2221 ctgggcgttt ttgtaccgac ccttaaatca ctgaagaaca gtaaaaaaaa tacactgtca
     2281 cgtactgatg ccacggaaga gctgacacgt ctttccctgg cccgtgctga gggattcgat
     2341 aaggttgaga tcaccggccc ccgcctggat atggataacg atttcaagac ctgggtgggg
     2401 atcattcatt cctttgcccg ccataacgta attggtgaca aagttgaact gccttttgtc
     2461 gagtttgcaa aactgtgtgg tataccttca agccagtcat cacgcaggct gcgtgagcgc
     2521 atcagccctt ccctgaaacg cattgccggt accgtgatct ccttttcccg caccgatgag
     2581 aagcacaccc gggaatacat cacccatctg gtacagtcag cctactacga tactgaacgg
     2641 gatattgttc agttacaggc tgatccccgc ctttttgaac tgtaccagtt tgacagaaag
     2701 gtccttctcc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc ccaggcactc
     2761 tacaccttta tagagagcct gccccgggat ccggcaccgg tatcgctggc gcggctgcgt
     2821 gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag acgggcaatg
     2881 gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg gcggacaaaa
     2941 ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga gagtaaggaa
     3001 aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg agatggcgga gaagcttgcc
     3061 ctgcttgaga aactgggcat cacgctggat gacctggaaa aactcttcaa atcccgctga
     3121 acataaactg tagtcagtga agagtgttcc tttactgact acagcttata ttatcaggtg
     3181 cagtgagtgg tctgctcact gcagtttata ttcagtttcc tgcagtgctg cctgtagctg
     3241 agctgtcatc tgccggtccc ttacgtgagt caccccgtaa cctgatgctg aggcattgct
     3301 cccttcataa aacatgactt actcactaca gcttatatac atgctccagc ttatgttatg
     3361 tctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct caggggaggc
     3421 agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac tgccagcgtg
     3481 gcagccatat ccagcgcaaa cactctggta taaacctcca tcgaacgtgg atccctgtga
     3541 cctgccaggg cctggatgac tttccggggc tggcggtgat agagcatatg catgatatag
     3601 ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtactccgtc agcttcggcc
     3661 cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacggc ccataatggt
     3721 tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac atagcttata
     3781 tccgtcagcg gaaccaggcg gacttcatct tttggcgggc gtccgcgtcg cgcacgcact
     3841 ttttcggaca ggatccgcac aaacggcctt gctccatcca ggtcaaatga ttccggcgtc
     3901 agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa cagcatatgg
     3961 tgacgctgat ccggcatata gaaaagaagg gcactcactt ccggggccag caggtaggcc
     4021 ggtgtggccc ctgtggaagt ggccattctt ctcagggaga gcgctgttgc aaaatccacc
     4081 cccggcgcaa tgggtaacag gaagacgcgt tctggtgaat tctgcagggg aatgacattg
     4141 ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta ttctgacctg
     4201 aacgcccgga tccttccacg acaataaatc gcagcgtgcg gatccttttg tttgcttatc
     4261 acgcattatt gcgggtaaaa ccaaatacgt aaaggtacgt tttgcagcag ggatcccgta
     4321 ttaagccagt cgtggcgcgg gctgagggct ggtttgccgt ttttgggaga attggggtaa
     4381 tgccaggacg ctaatgatct atcaatggat ggatgtaagt gacaattgaa aactgaaaaa
     4441 tgcgctatgt agattctgac agatttatgc acctgaaata cgttcaaaat ccacatagcg
     4501 taactctgat tttccttacc agaaaaaaga gggggaataa ccagtatct
//
LOCUS       DBFIFS010000282         4541 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.283, whole genome shotgun sequence.
ACCESSION   DBFIFS010000282 DBFIFS010000000
VERSION     DBFIFS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.283"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1305
                     /locus_tag="N7A07_004434"
     CDS             <1..1305
                     /locus_tag="N7A07_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU2478963.1"
                     /translation="LPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSI
                     GPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVV
                     NVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGI
                     AIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLII
                     GILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLI
                     GLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRG
                     NLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQGGSPITWLLI
                     QVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLAE"
     gene            1353..2393
                     /gene="gatD"
                     /locus_tag="N7A07_004435"
     CDS             1353..2393
                     /gene="gatD"
                     /locus_tag="N7A07_004435"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU2478964.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            2499..3272
                     /locus_tag="N7A07_004436"
     CDS             2499..3272
                     /locus_tag="N7A07_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2478965.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(3354..4253)
                     /gene="yegS"
                     /locus_tag="N7A07_004437"
     CDS             complement(3354..4253)
                     /gene="yegS"
                     /locus_tag="N7A07_004437"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU2478966.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1106 a         1021 c         1192 g         1222 t
ORIGIN      
        1 ttgccgattg tcatcattat tttttctaaa attttaggca tgaaggcagg cgattgcttt
       61 aaagcgggtc tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg
      121 ctggattcca ttggcccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat
      181 gtggtcgatg tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg
      241 gtggcgattc cgattgcgat tctggttaac gtggcgatgt tactgacccg tatgacgcgg
      301 gtggtaaatg ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcat
      361 ctggcaaccg gttcatggat gatagggatg gcaggtgtgg taattcacgc ggcgtttgtt
      421 tataagctcg gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggcatt
      481 gctattccgc acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc
      541 atcgagaaaa tcccaggcgt taaccgtatt aaatttagcg ccgacgatat tcagcgcaaa
      601 tttggcccgt ttggcgagcc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc
      661 ctcgcgggtt acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcggtgatg
      721 ctgctaatgc cacgggtgat taaacccatc atggatggtt taacgcccat cgctaagcag
      781 gcccgtagtc gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc
      841 gcattactgc tggggcatac ggcggtggta tcggcaagcc tgatttttat tccgctcacc
      901 attttaattg ctgtttgtgt tcctggtaat caggtactgc cgtttggcga tcttgccact
      961 atcggcttct tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta
     1021 atctcgggtg tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg
     1081 cacacccaac tggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg
     1141 gatcagggcg gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt
     1201 cccggtttca ttattatcgg cgcaatttat cttaccggta ttttcatgac ctggcgtaga
     1261 gcgcgtggct ttattaaaca agagaaagtc gttctcgcag aataattttt acctgagggg
     1321 gaggttatcc cccttaacaa ttaggagttt ttatgaaatc agtggtgaat gatactgatg
     1381 gtatcgtgcg cgttgcagaa agcgtcattc ctgaaattaa acatcaggat gaggtgcggg
     1441 taaaaattgc cagctcgggc ttatgtggtt ccgatttacc caggatattt aaaaatggtg
     1501 cacattatta tccaataacg ttaggccatg aatttagcgg ctatattgat gcggtaggat
     1561 ccggtgttga tgatttacac cctggcgatg cggttgcctg tgtgccgtta ttaccctgtt
     1621 ttacttgtcc agagtgtttg aaagggtttt attcccagtg cgcaaaatat gattttattg
     1681 gctcacggcg tgatggtgga tttgccgaat atattgtcgt taagcgaaaa aatgtctttg
     1741 ctctacccac ggatatgcct attgaggatg gggcttttat tgagccgatt accgttggcc
     1801 tgcatgcttt tcatttagcg caaggttgtg agaataaaaa cgttattatt attggtgccg
     1861 gaaccattgg cctgctggct attcagtgcg ctgtcgcgct gggagcaaag agtgtgacgg
     1921 ctatcgacat tagctcagaa aaactggcac tggcaaaatc tttcggtgcg atgcaaacat
     1981 ttaacagtag cgaaatgagc gcgccgcaaa tgcagagcgt tttacgcgaa ctgcgcttta
     2041 atcagcttat cctcgagacg gctggcgtac cgcaaactgt cgaactggcg gtagagattg
     2101 ccggtcctca tgcccaactg gcactggtgg gcacgttgca tcaggatctg catttaacat
     2161 cggcaacgtt tggcaaaata ttgcgtaaag agctgacggt tatcggcagt tggatgaact
     2221 actccagccc ttggccgggg caggagtggg aaacggcgag ccggttgctg acagaacgta
     2281 agttaagcct ggagccatta atcgctcacc gtggaagctt tgaaagcttc gcccaggcgg
     2341 tgcgtgacat cgctcgtaat gctatgccgg gcaaagtgtt gctcattccc tgaaaccgcg
     2401 ggccagcgtg atgctggccc ggtattgtgc aaaacagatc attcaccaat ggtccccctt
     2461 cgtttacact agccacaatt gaatatgggt aaatgacgat gaattcattc gagcgaagga
     2521 ataagatcat ccaattagtg aatgaacagg gaaccgtgct tgttcaggat ctggcgggag
     2581 tatttgctgc ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa caaaaaggcg
     2641 tggttacgcg ctttcatggc ggtgcggcga aaataatgtc tggtaatagt gaaaccgaga
     2701 cccaggaagt cgggtttaaa gagcgatttc agcttgccag cgcgccaaaa aacagaatag
     2761 cgcaggcggc agtcaaaatg atccacgaag ggatgaccgt tattctcgac agcggaagta
     2821 cgacaatgct tatcgccgaa ggattaatga ccgccaaaaa tatcacggtg attaccaaca
     2881 gtctcccggc agcgtttgcc ctttccgaaa ataaagacat tactctggtc gtctgtggtg
     2941 gcacggtccg ccataaaacg cgctcgatgc atggttccat tgccgagcgt tcattgcagg
     3001 atattaatgc cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt aatggtatta
     3061 cgacctttaa tgaaggttat tcgattagcg gggcgatggt caccgccgca aataaagtga
     3121 ttgccgttct cgattcatcg aaatttaatc gtcgcggttt taatcaggta ctgccaatcg
     3181 aaaaaattga cattattatt accgatgatg ccgtgtcaga tgtggataaa ctggcattgc
     3241 agaagacacg ggtaaaatta attacggtgt agtattgttc tgtatgccgg ataaggtgtt
     3301 tacaccgcat ccggcattac ggattctccc tggttcaaga gagaatagtt tgattaacgc
     3361 aataatggac aatctggtgg taatcgacaa cgcaacgccg ccggaagtat ttcaatatgg
     3421 aagttttgcc cactcaacgg ttcgccatca agattaaaag tgatttcgtg tggggcttgt
     3481 atatcaaacc acgacgaagc gccttcgata atattcgggt tatcttcgtc agattttaag
     3541 gttgatacga gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg cagcaagcca
     3601 tcgttaatta acgcgttcgg gcacaattgt tgaccgccac cggcctgacg cccgttacca
     3661 ataccaatga ccagggcgtc accttgccag tgaaagtttt caccgcggat ttcacaacgg
     3721 tccggttgca gagtatccat gcgcattaag ccatgaatga tgtaagagac gccacccagc
     3781 gcggctttta atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc gcctgtcgcc
     3841 atattaataa aacaggtttg tttgttgacc tgcgccatat ctatcgcaat ggcgttaccg
     3901 gcaattgcca gtttcagcgc cttatccagt gcctcaggaa tccctacact ggtggcaaaa
     3961 tcattggcgg ttcctaatgg caaaattccc agcgcgggta tgtcatcccc ctcacactga
     4021 atcaacgccg tagaaacttc attaatggtg ccatcgccac caccggcaat caccgttgcg
     4081 acgcccaact tccgggcctc ctctacatat cgtgcggcat cgcctttctc ccaggtgacc
     4141 cgcacatgga tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg caagggtaga
     4201 ttgtcagtac ttttgccatt aagaatcagt aagctggcgg gaaattctgc catgatcgta
     4261 tgtgccttta tgattggtct atagatagtg tagagcagaa aagaaaaggt ggagtcagaa
     4321 caggatgaaa gtcggaggat aaatagtcag ttcaagtatg gaggattcag tggttaccac
     4381 caggagcttg caatggggac agcgaatact aacggcagcc accgataaat ttcaaaaaag
     4441 agcatatacc taatattcaa ctaaacagtg gcttcttcaa tataatatat taaagcctcc
     4501 atggagttac ccggaagggc ctccatgtcc gtaattccta c
//
LOCUS       DBFIFS010000283         4464 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.284, whole genome shotgun sequence.
ACCESSION   DBFIFS010000283 DBFIFS010000000
VERSION     DBFIFS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.284"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            514..1287
                     /locus_tag="N7A07_004438"
     CDS             514..1287
                     /locus_tag="N7A07_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478967.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(1345..1899)
                     /gene="pinE"
                     /locus_tag="N7A07_004439"
     CDS             complement(1345..1899)
                     /gene="pinE"
                     /locus_tag="N7A07_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HGU2478968.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            1962..2423
                     /locus_tag="N7A07_004440"
                     /pseudo
     CDS             1962..2423
                     /locus_tag="N7A07_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2423..3016
                     /locus_tag="N7A07_004441"
     CDS             2423..3016
                     /locus_tag="N7A07_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2478969.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(2988..3431)
                     /locus_tag="N7A07_004442"
     CDS             complement(2988..3431)
                     /locus_tag="N7A07_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HGU2478970.1"
                     /translation="MDEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGDELTQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(3452..4162)
                     /locus_tag="N7A07_004443"
     CDS             complement(3452..4162)
                     /locus_tag="N7A07_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2478971.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALQNLGLGEAAKRDVGTGENQIPDMASFASGNGWMKLPNGKILQYGRGAVTP
                     TLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVMTAEGPTLTGFNSAYRT
                     SSTSDTVSMNYSWWAVGE"
     gene            complement(4162..>4464)
                     /locus_tag="N7A07_004444"
     CDS             complement(4162..>4464)
                     /locus_tag="N7A07_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
                     /protein_id="HGU2478972.1"
                     /translation="IAAQMVNFDREQMRRIANIDNQDMTISVWVFPDTDISDVSRELI
                     AAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFDVGAWGVLL"
BASE COUNT         1205 a         1078 c          998 g         1183 t
ORIGIN      
        1 ccccttctct acgcaacttc agttcccacc accaactttg cggcagcttt gtaggatcaa
       61 tgtctaaaag aataatggtg accgataaga aaacgactga ataactgcag attttcgctc
      121 aaaaccttcc tgtcagatcc atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa
      181 aacagtgacg atctaaccct tcaagaatat tctacgattg ttctgtttag gaaaagcaag
      241 acgggaagtc gggagataag tcattgataa agtggcggag agagggggat ttgaaccccc
      301 ggtagagttg cccctactcc ggttttcgag accgacccga tcatcaaacg aaacataaaa
      361 ttagctcaca ttatgaggaa aggtatcttt ttgcgctatg taaattcaaa gggttagcct
      421 cattttcccg atggctttct caacactact agttgtgagc ccttgcaatg ttcattaata
      481 tacgtctcac aaataattca cagatattgc aaaatggata ttactgagtt tccttctgga
      541 gtaattgaac accttggctg gtatgtatac cgattgattg atcctaggga cggaagcacc
      601 ttctatgtag ggaaaggcaa aggtaaccgc gtatttgccc atatgcgcgg tgaagtggca
      661 gcggctgatg atgacgactt actgagcaac aagctaaagc aaatcagaga aataagatta
      721 gcgggacttg aagttatcca tgtcatccat cgacatggaa tgactgatga aaagacggcg
      781 tacgaagttg aagcagcact tattgatgcc taccctgggt taacgaatat catgaatggt
      841 gctggcagca atgaattcgg cgccgcgcat gtcaaagagt tgatagcaac atatcaaccc
      901 gaaaccataa catttcatca taaagcatta atgatttccg ttaacagaag tgcaaaagat
      961 tcagagcttt atgatgcggt tcgatttagc tggcgcatta atgtctcgcg cgccagtaaa
     1021 gcagaagtca ttcttgctac tgtaaggggg atcgttcgag gagttttcat tgctgataaa
     1081 tggctaaaat caacgcgtga acattttcct acgatgaaat actgggacga agaccctgac
     1141 tttgaggcaa cacaaagctc tcgctatggt tttgaaggtc gagaagcccc acctgaaata
     1201 gcaaaccttt atcttgggaa aaaaatacca gatgaattaa gaaaaaaagg agctatgtct
     1261 ccagttcgtt actcacctaa tttttgaatc ttcaagtggt aagcataaac cgcagcacga
     1321 tcttgcatac gacgtgctac ggtttcattt atctccggcc ggaaacttct tatacagtgt
     1381 cgatacacca acatcataga tgatcgctac cttctggcga ggaattcctg ctgcaattaa
     1441 tcgtccggcc tgcgcccatt gttctggtgt aagtttggga cgacgtccac caattcgtcc
     1501 ctgtgcgcga gcagcttcca gtccagcttt tgttcgttca acaatcagtt ctcgttccat
     1561 ttcagccagg gcccccatca catgaaagaa aaagcgcccc attggggtac tggtatcaat
     1621 agcatccgtc aggctgcgaa aattaacgcc acgttcgcgc aactcctcaa ccagaatgac
     1681 cagatgccgc atactgcgcc ccagccgatc cagtttccag acaaccagag tgtcacctgc
     1741 cgataatgtc ctgagcagtt ttttcagtcc cggcctttcg gactttgtac cgcttatctt
     1801 gtcttcaaaa atcagctcgc atcctgcaca gttcagcgca ttacgttgta gatctgtgtt
     1861 ctggtcattt gttgacacac gtacatagcc aataagcatg gtagatctcc ctgacaaaag
     1921 caggaatgat gccatttgct cgttatttct gcattttcat aaacgttggt ttgggagaag
     1981 gtgctccagc tattggcgtt ccgttcttct ggccgtccgc tgcaatgcca aatactgtaa
     2041 tcgacagctg gtcctgtatg gtgtttttga agttcaacgg cgcgaaattc tctgccactg
     2101 attatcctgt gctggcgaaa gtgtttcctt cgctggtatt acctgaagcc cgcggtgatt
     2161 tcattcgtat ctgggatgac gggcgaggtg cagacggtgg tcgcgaatta ttaagctggc
     2221 aggcagctac aaacttttct cagtttgccg ggaatatagg cgaaggtgcg ggacacgcaa
     2281 ttaactttca tgatggcatc gccggaaatc agccaggatt ttcacgattt aatttcacca
     2341 gtaactctgt gggtgatggt gtgaattttg ttgcagtcag accgcgaaat attgcattta
     2401 actttctggt gagggctaaa taatgaaacc tgtttttgat gaaaatgggc tggctacagt
     2461 gccgggcgat atgcgttgtt tttattatga tgctgaaaca tctgagtata cgggctggtc
     2521 tgatgaatat attaatactg gcgtaagtat gcccgcctgt tccactggta ttgaccctgg
     2581 cgaaaacatt ccgggaagag tggcagtatt tacaggtaag ggatggagcc atgaagaaga
     2641 ccatcgcaat gagactgttt actcaatcga aaatggcgca gctgttacag tggattatat
     2701 cggtgccatc aaagacggtt atgtcacgat ttcaccgtta acgccatacg ataaatggga
     2761 tggtgagaaa tgggtgacag acactgaggc acaacacagt gccgcagtag acgcggcaga
     2821 agcacagcgc caatcactga ttgatgcagc aatggcttcc attagtctga ttcagctgaa
     2881 gttacaggcc ggacggaaac tgactcaggc agaaacaacc cgacttaacg ccgtgctgga
     2941 ttacattgac gcggtgacgg caacagatac cagcaccgcg ccggatgtca tctggcctga
     3001 actgccggag gcgtaggcca ttcaatatct ggcgcaccgg aagtatcgac cagctccagt
     3061 gcgtccagat aatccagcca caaattatat tgcgtcagtt cgtcaccttt cagacgacca
     3121 atagccgctt taccgggcca ttgcttactg ttcatgtatt cgttggcctg gttaattagt
     3181 agctgtcttt ctgattcagt aatttcaata agctcttcat gcgtgggtgg aggaatatct
     3241 gcccacgcag gcagcccatc atctccggca atacggattt ttccttgtgg cggttcagcc
     3301 ataaactcac tgataatatt ttgatttact tccttagcgt ctgataaatc ccatccctct
     3361 gatttatatt tatcaatcat atccacaggg aaaaaagcat tatgccttgc gctataaaca
     3421 tattcgtcca tataaatcac cctgaataaa attactcacc aacagcccac caactgtaat
     3481 tcatcgatac cgtgtcgctg gttgatgacg ttctgtaagc agaattaaag ccggttaacg
     3541 ttgggccttc tgcagtcatc acgaaccctc gcccagcgcc taaaggcgca ccgccatcac
     3601 cagaatgagt aagcatggcg cagtccgctt ttttggggaa agggatgctg aatgtaattc
     3661 tcattgtttg cgtcgataat gtcggcgtaa ccgcaccacg accatattgc aggattttcc
     3721 cgttgggtaa tttcatccat ccattaccac tggcaaaaga ggccatgtcc ggtatctgat
     3781 tttcccctgt ccccacatcc cgttttgccg cttctcccaa accaaggttt tgaagagccg
     3841 ttttcaccgt gccgtccgat ttgatatcgc caaacggatt cttgcggctc aggtattcaa
     3901 cagcaaaccc cgatcccagc aattcaacaa aaccgggcag atcaccatta tcaagcacat
     3961 cccgttgcgt tttatcactt acaaactggg ccagagctgc agcaataaag ctggcctgcc
     4021 gaataacctt attgacttgc gcactggatg ctttccctgc tgtaaatccg gatataagcg
     4081 caggcaacgc ttcccattcc tcctgcgaca taacattggc atttcgatca gttgcaaacg
     4141 ctttaaagtc atttttcgcc atcagagtaa tactccccat gcccctacat caaaaccact
     4201 gatgaattcg ttatccatat caaaaccaaa aaatttagag ccttccgatg gagtttccac
     4261 cgaaggtgtt tcaatgcccc ccgcccatac cccggcggct tttactgtta gatacccctg
     4321 tttaattgcc gcaattaact cacgcgatac atctgaaata tcagtatcag gaaagaccca
     4381 gaccgatatc gtcatgtcct ggttatcgat gttggcgatc cgacgcatct gctcacggtc
     4441 aaagttaacc atctgtgcgg cgat
//
LOCUS       DBFIFS010000284         4459 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.285, whole genome shotgun sequence.
ACCESSION   DBFIFS010000284 DBFIFS010000000
VERSION     DBFIFS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.285"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..454
                     /gene="ydiV"
                     /locus_tag="N7A07_004445"
     CDS             <1..454
                     /gene="ydiV"
                     /locus_tag="N7A07_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU2478973.1"
                     /translation="PQVATLLLERDNYAGELLKYPFIELLINENYPHLNEGKDNRGLL
                     SLSQVYPLVLGNLGAGNSTMKAVFDGLFTRVMLDKSFIQQQITHRSFEPFIRAIQAQI
                     SPCCNCIIAGGIDTPEILAQITPFDFHALQGCLWPAVPINQITTLVQR"
     gene            517..1953
                     /gene="selO"
                     /locus_tag="N7A07_004446"
     CDS             517..1953
                     /gene="selO"
                     /locus_tag="N7A07_004446"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU2478974.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1957..2148)
                     /gene="hemP"
                     /locus_tag="N7A07_004447"
     CDS             complement(1957..2148)
                     /gene="hemP"
                     /locus_tag="N7A07_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU2478975.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2280..3326)
                     /gene="aroH"
                     /locus_tag="N7A07_004448"
     CDS             complement(2280..3326)
                     /gene="aroH"
                     /locus_tag="N7A07_004448"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU2478976.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3483..4316)
                     /gene="ppsR"
                     /locus_tag="N7A07_004449"
     CDS             complement(3483..4316)
                     /gene="ppsR"
                     /locus_tag="N7A07_004449"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU2478977.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1092 a         1057 c         1156 g         1154 t
ORIGIN      
        1 gccacaagtg gctacattat tattagagag ggataattac gcaggcgaac tattaaaata
       61 tccgtttatt gaactgttga ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa
      121 tcgcggtctt ttatccttat cgcaagtgta tccccttgtg ttaggtaatt tgggagcagg
      181 caacagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtaatgc tggataaaag
      241 ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg cgattcaggc
      301 gcaaatttcc ccttgttgca attgcatcat cgcggggggc attgatacgc cagaaatatt
      361 agcacagatt acccccttcg atttccatgc tctccaggga tgcctgtggc ctgccgttcc
      421 cataaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg tgtttaagac
      481 gagagtaacc gtctacacta tcaaacagga ggctctatga ccctgtcttt tattacccgc
      541 tggcgcgatg aattgccaga aacctataca gcactttccc ctacgccttt aaataatgcc
      601 cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc gtcgctgttt
      661 aaaaatggcg caggtgtctg gggcggcgaa accctactgc ctggtatgtc accactggcg
      721 caggtttaca gtggtcatca gttcggcgtc tgggcgggcc aactgggtga tgggcgcggc
      781 attttacttg gcgaacaact gcttgctgat ggcactacaa tggactggca tctgaaaggt
      841 gctggcctga cgccttattc gcgaatgggt gatggacggg cggtattacg ttcgacgata
      901 cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac ccgcgcgtta
      961 agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg cgcgatgctg
     1021 atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg aacattttta ctatcgccgc
     1081 gagccggata aggttcgtca gttggctgac tttgccattc gtcattactg gtcacatctt
     1141 gaagatgatg aggacaaata ccgtctctgg tttaacgatg ttgtcgcacg taccgcatcg
     1201 ttaattgccc aatggcagac ggtcggcttt gcacatgggg tgatgaatac cgacaacatg
     1261 tcgctgctgg gactgacgct tgattacggg ccgtttggtt ttcttaatga ttacgaaccc
     1321 ggttttattt gtaatcactc ggatcatcaa ggacgttaca gctttgacaa tcaacctgct
     1381 gtcgcgttgt ggattttaca gcgtctggcg cagacattgt caccatttgt tgccgtagat
     1441 gccctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta tggacaacgg
     1501 atgcggcaga aactgggctt tatgacggag caaaaagagg ataacgcgct actgaatgaa
     1561 ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacatttcg catgctgagt
     1621 ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg agtttattga tcgtgcggca
     1681 tttgatgact ggtttgcccg ttatcgaggg cgtttgcaac aagacgaggt tagcgatagt
     1741 gagcgtcagc aactgatgca aagcgttaac cccgctctgg tattgcgcaa ctggttggcg
     1801 caacgggcga ttgaagcggc agaaaagggt gatatgacgg aattgcaccg cctgcatgag
     1861 gcgttgcgaa atcctttcag cgacagagat gatgactatg tcagtcgtcc acctgactgg
     1921 ggtaaacggc tggaagtcag ctgctcgagt taaaggctac ttggtcaaca gcagcttgcc
     1981 agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca gtttgccatc
     2041 cggacctaac agtgtctggc tggaaattcg ccgaataggc tgcggggaag cggtcttgtg
     2101 attggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa aaattaataa
     2161 taattgatat aacaatgatt cttattatca atattatggc tgaccagagc aagcagtaat
     2221 gtgttgagaa gacaacaagt tgcgcagaaa tgcccatccc atcaggaatg ggcacgcatt
     2281 cagaagcggg tatctaccgc agaggctagt ttttcgacca ggcgttcggt atcttcccag
     2341 cccagacacg ggtcggtaat ggattgaccg taagtgagcg gctgaccgcc gacgattttt
     2401 tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt agagccattg
     2461 cggatttgct gacaaatatc ctcacaaact tctaactggc gacggtgctg cttctggcag
     2521 ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg cagcgtatcg
     2581 caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg cataataatg
     2641 tggccatacg ggttgccgct ggtctgatag atggtcatct gaccattttt gtctggcgag
     2701 aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cagcaatccg cgtattgcca
     2761 tcggtaccat ttttaaaacc taccggacag gagagtgccg aagccatttc acggtggatc
     2821 tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc aataaactga
     2881 ccggtcacca tatcgaggaa ctcagtcgcg gttgggacgc ccagctcatt tacctgtaaa
     2941 agtaatttgc gcgccagctc cagaccgtga tttatccgat agctgccgtt taaatctgga
     3001 tcggagatta gtcctttcca gccgacaaca gttcgtggtt tttcaaaata ggtgcgcatt
     3061 acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt ggcgtactcc
     3121 attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag tcgcttatct
     3181 tcaccattga gtattttttc aattctgcgg cgggagtcgg tgacatgggt ggcgacgcca
     3241 ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc aatacgcgca
     3301 gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct tctacggcag
     3361 gactgccggg aagtgatgcg catcacgata aaccaatcgc tgctgatttc aagtgcgagg
     3421 tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aataagctaa cgcactagtt
     3481 ctctagtaca ttcggcgact aaggcccatg atatcgagga tcttggtggc aatctcttct
     3541 accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa ggcttccact
     3601 tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc ccgacgttcc
     3661 tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt atgctgaagc
     3721 ggtttgagcg acgcgggtag caccagatta tccatatcgt cggcaataaa ggggtagttt
     3781 gccgcgcgga taccaaattg cattgccaga tacagactgg tgggggtttt accacagcga
     3841 gaaacaccga gcaggatcac ctgagcctga tccagattgc gcaacgaaat gccgtcatcg
     3901 tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat atttattgag attattaggg
     3961 ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc ttgttgtagc
     4021 ggggcaacca gcgcctgaac gatatcctgg caaaaacctt cactttgcaa gatgatggcg
     4081 cgaatttccg gcaacacgat ggagtagaag accagcggac gcacgcctgt ctggtgataa
     4141 attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac aaacggcagc
     4201 gtgatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa tacctccgca
     4261 gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt atccattttg
     4321 ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc acagaagcgt
     4381 agaacgttat gtctggttta taaaatgaac cttcaatttt attttttatg aaaacagcat
     4441 ttcattttta tggtttcgt
//
LOCUS       DBFIFS010000285         4330 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.286, whole genome shotgun sequence.
ACCESSION   DBFIFS010000285 DBFIFS010000000
VERSION     DBFIFS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.286"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            68..1174
                     /locus_tag="N7A07_004450"
     CDS             68..1174
                     /locus_tag="N7A07_004450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU2478978.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            1264..2985
                     /gene="cptA"
                     /locus_tag="N7A07_004451"
     CDS             1264..2985
                     /gene="cptA"
                     /locus_tag="N7A07_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU2478979.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            3059..4057
                     /gene="lpxM"
                     /locus_tag="N7A07_004452"
                     /gene_synonym="msbB"
     CDS             3059..4057
                     /gene="lpxM"
                     /locus_tag="N7A07_004452"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU2478980.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            4110..4298
                     /locus_tag="N7A07_004453"
                     /pseudo
     CDS             4110..4298
                     /locus_tag="N7A07_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1086 a          988 c         1099 g         1157 t
ORIGIN      
        1 attatacaac gccacttttt tcttcagtac tggctcttca taaggggcca agattaccag
       61 ataattaatg atgaaaattt tattcactga atcttcgtca gatattgggg gccaggagct
      121 gcaggcactg gcccagatga ctgctcttca aaagcaggga cattccgttt tacttgcctg
      181 cagagaaaaa agtaaaattg ctccggaagc cagaaaaaga gggcatgatg tcacttttat
      241 tcctttcagg aacagccttc accttccttc catcctcaga cttcgacgga ttatcgggga
      301 atttaaacct gatctggtca tctgccatag tggccatgac agtaatatag caggactgag
      361 ccgtctgata tgctgtcacc gcttcagtat tgtcagacaa aaaacctaca tcacccgtaa
      421 gaccagaacg ttttccctga attatctgtg tgatttcatt gttgtgccta gctcagccat
      481 gatggcgcat ctgatggcgg aaggtgtcag gacaccggta acggttattc caccgggttt
      541 cgactggccc gcattgcata atgaagccat gcgaccgtta cccctgcata tccacgcctg
      601 ggctgcatca gcagacaatg ttccgcttat cgttcaggtg gggatgctac gtcccgaaaa
      661 aggacatgaa tttatgctgc gtgtattgta tcagctgaag atggaaggga aatcatttcg
      721 ctggctcgtg gtgggcgccg ggcgtgaaga atacgaagca cgtctgcgtc agcagacaga
      781 acacctggga atgagcggag atgtgttaat ggcaggtgcc cttttcccag cgttaccggt
      841 ctaccggatt gccagtgtgg tggtcatgcc ttcggaaaat gaagcgtttg gtatggtact
      901 ggctgaagcg tcagtctccg gcgtgcccgt tatcgccagt gagacgggcg ggatccctga
      961 tgtcattcag aagaatgtga cagggacgct gttacccgtg ggggatgtgt ctgcatggac
     1021 cggcgcactg cgcgattttt tatcccgacc ggaacgcttc cggatgatgg cagccagcgc
     1081 cagagaagat attgagtacc ggtttgatat taacagaacg gcacagatca ttgtgtctct
     1141 ggctagtcag gctaagggga aatgcaacag ataacggcgg atcctctttt cagcccgaat
     1201 tttgttcagc ggttttccgg taacggagta atttatcaca ttgtttaaat acggggacgg
     1261 tcaatgcatt taaataccgg acagaacagg cctactttca gctggagcgc gctgggatgg
     1321 gcaatttttt attttggctt tttttccact ctcctgcagg tcatcatctt cagcagtggg
     1381 tacagcggaa cgaatggaat acgggactca ctgttattca gttgtctgtg gttgatcccg
     1441 gtgtttctct atcctgatcg gataaaaata attgcagctg ttgtcggttt cattctctgg
     1501 ggcacgtcgc tggcagcact ttgttattat tttctctatg gtcatgaatt ctctcaaagt
     1561 gttcttttcg ttatgtttga aacgaatgcc agagaggctg gtgaatattt cagccagtat
     1621 ttcagcctta aactgttgct tatctcgctg gtatatactg cggtgtccgt ttttctgtgg
     1681 acacgtctgc gtcctgtata tattcctttg ccatggcgga gaattgtctc tttcctgctg
     1741 ctttatgctc tgcttctgca tccggttgtt ctgaaatcgt taatcagaca ggagccgctg
     1801 aatgatactc taggcaaact ggcatcccga atggagcctg ctgccccctg gcagtttgta
     1861 tccagttatt atcagtacca tcagcaactg aatgcactga caaccttcct gaatgaaaat
     1921 agcgcactgc caccactggg taatctcagg gatgaatcag gggagagacc acgcacactg
     1981 gtcctggtga ttggtgagtc gacacagcgc gaacgcatga gcttatacgg gtatctacgt
     2041 gaaacgacgc cggagctgga tgcactgcgt aaaaccgatc cgggtcttac tgtgtttaat
     2101 aatgtggtgg catcgcgtcc gtacaccatt gaagcattgc aacaggccct tactttcgcc
     2161 aacgaaaaga atcctgatct gtatctgacg cagccgtcgc tgatgaacat gatgaagcag
     2221 gcaggctata aaacattctg gattaccaac cagcagacaa tcacagcccg taacaccatg
     2281 ctcactgtat tttcgcgcca gacggacagg cagtactaca tgaatcagca gcgaacacaa
     2341 agtgcgcgtg aatatgacac taacgtgctg aagccgttcc gggaagtgct gaatgaccct
     2401 gcaccaaaga aactgatcat cgtgcatctg ctgggtacgc acattaagta taaataccgt
     2461 tatccggaag gtcaggggcg gtttgatggc attacagggc atattcccac tggattaaat
     2521 gcgaaagagc tggaagtgta taacgattac gataatgcca atctgtttaa cgatcatgtg
     2581 gtggccagtc tgataaagga cttcagagca actgcgccgg acggctttct gctttatttt
     2641 tcagaccatg gtgaagaagt atatgatact ccgccatata aaacgcaggg acggaatgaa
     2701 gataacccca cacgtcccat gtacactgtt ccgttcctgc tgtggacctc ggaaaagtgg
     2761 catgctgcac atccgcgaga tttttcgcag tatgttgacc gcaaatacag tctggctgaa
     2821 ctgatccaca cctggtcaga tttggcggga ctgacatacg atggttacga tccgacccgt
     2881 tctctggtga atccgcaatt cagggaaacc acccgctgga ttggaaatcc gtataaaaag
     2941 aatgggctca ctgatttcga cactcttccg tatggtgagc catagtattt tcttatcttc
     3001 ggttccgttc tgaaggcagg aggatgtgtt ttcctctgct gtccggtggc ctccagggat
     3061 ggtaccacct tctgctgtac tctgttatca taatgagata agcaggcaga taccggtaaa
     3121 tatgaaaaat atcagaactg agtttattcc ccgctttaac cttacattat gcttcccccg
     3181 ttactggatg acgtggacag gtatcggtat tatctgtgtg tttgcaatgg tcccccccgc
     3241 actgagagat ccattgttgg gaaaattggg catgctggtt gggcgtctgg ggaaaagcgc
     3301 acgtcaacgg gccttgatta atttatcgct ttgttttccg gagtacagtg ataaagaaaa
     3361 ggagaatatc gttgatgcca tgtttgcaac agcctccatg gccgttgtgc ttatggctga
     3421 attagcactg tccgggcctg ataaaatttc gcatcgtata cgctggaatg gtctggagat
     3481 tgtggagaag atggcacaga acaatgaaaa ggtcattttt cttgtgccac atgcctgggg
     3541 agtggatatt cccgcaatgc tgatggctgc gtcgggcaga aaaatggcag cgatgtttca
     3601 taaccagaga aatccggtgg tggattatgt ctggaacagt gttcgtcgtc gtttcggagg
     3661 aaaactgcac gcacgtaatg acggtattgc ctcctttgtc cgctcagtcc ggcagggata
     3721 ctggggatat tatcttcctg atcaggatca tggcccggaa ttcagtgagt ttgcagattt
     3781 ttttgcgacg tataaagcaa ctttaccggt tatcggacgg ctcagcagga tcagtggtgc
     3841 acggataata ccgcttttcc ctgtttatga cggaaagacc catcatctga ctattcatgt
     3901 cagcccgcca cttgctatac gacagaagag tgatgctcat attgcgcgac agataaacga
     3961 agtggttgaa aatttcgttc gtccacatcc tgagcagtat acgtggatcc tcaagttgct
     4021 gaaaacgcgt aaagaagggg aagaggatcc ttactgaggc agctttttca ggataatgag
     4081 cttacccagc aatagtggac acacgctaag tgagtaaact ctcagtcaga ggtggctcat
     4141 atgacaaaaa aacagtacca accagtaaaa aaccccgtaa atagcattcg cctgaatttc
     4201 gcagtgaagc cctgaagctt gctgaacgca tcggtgttgc tgtcgcagct cgtgtgctca
     4261 gtctgtatga atcacagctt gtaagcggct cgtcagaact gtattgatat ttactgagcg
     4321 gtcagttcaa
//
LOCUS       DBFIFS010000286         4330 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.287, whole genome shotgun sequence.
ACCESSION   DBFIFS010000286 DBFIFS010000000
VERSION     DBFIFS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.287"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..274)
                     /gene="chbB"
                     /locus_tag="N7A07_004454"
     CDS             complement(<1..274)
                     /gene="chbB"
                     /locus_tag="N7A07_004454"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU2478981.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLG"
     gene            complement(574..912)
                     /gene="osmE"
                     /locus_tag="N7A07_004455"
     CDS             complement(574..912)
                     /gene="osmE"
                     /locus_tag="N7A07_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU2478982.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1114..1941
                     /gene="nadE"
                     /locus_tag="N7A07_004456"
     CDS             1114..1941
                     /gene="nadE"
                     /locus_tag="N7A07_004456"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU2478983.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2171..3058
                     /gene="cho"
                     /locus_tag="N7A07_004457"
     CDS             2171..3058
                     /gene="cho"
                     /locus_tag="N7A07_004457"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU2478984.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSQEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3018..3593)
                     /gene="ves"
                     /locus_tag="N7A07_004458"
     CDS             complement(3018..3593)
                     /gene="ves"
                     /locus_tag="N7A07_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU2478985.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3796..4281)
                     /gene="spy"
                     /locus_tag="N7A07_004459"
     CDS             complement(3796..4281)
                     /gene="spy"
                     /locus_tag="N7A07_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU2478986.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1066 a         1067 c         1046 g         1151 t
ORIGIN      
        1 cgcctaaacc atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg
       61 gtaacaaacg ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat
      121 cggcattctg acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa
      181 cttcatattt ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg
      241 cagaacaaaa cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct
      301 gtcagccaga cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc
      361 aacatgactt tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc
      421 tttgacctta taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc
      481 aaataattcg cgaagataat taagaactca ggccaaaaaa aaaccggcgc aatggccggt
      541 ttccgttgtt actcaagctt tcagacgaag tgattacttc gcagcctgtg gatcagtgtc
      601 gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac
      661 aaagtaggtt tctgctttac catcacgttg acccaggatg taggtctggc aagtaccacg
      721 agcatggatc atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg
      781 gctcatgcct tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc
      841 ataagccgta caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat
      901 attcttgttc atattccgtc ctattgttta tcagcgtgtt agataagcct ggaatacatt
      961 gggcgctttt tcaagcccat gaacgaaacg gctccgcttt cagaggattc ctgtatgacg
     1021 ttttaaccac cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa
     1081 ggaagttttg tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag
     1141 gcgctgggcg caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tgtcgatttt
     1201 ctgaaaagct acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc
     1261 ggtcaggact ccacgcttgc cggtaagctg tgccagatgg cgattaatga gctgcgccag
     1321 gaaaccggca acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc
     1381 gacgaacaag attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt
     1441 aatatcaagg gcgcggtatt ggctagcgag caggcattgc gggaagcatg cattgaactg
     1501 agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt
     1561 gcgggtatga ccagcggtgt cgtggtgggc accgatcatg cagcagaagc cattaccgga
     1621 ttcttcacta aatatggtga cggcggtacg gacattaatc cactgtatcg tctcaacaaa
     1681 cgtcagggta aacagttact ggcagcatta ggttgcccgg aacaccttta taagaaagcg
     1741 ccaacggccg atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg
     1801 acctatgaca atatcgacga ctatctggaa gggaaaaacg tacctcaaca ggtcgccaga
     1861 acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc
     1921 gatgatttct ggaaaaagta ataatttgca cctattggat tgtgcaaaaa agagtaattt
     1981 gttcacgccg gatgcggcgt gaacgcctta ttcgacctac aaaactatgc aaattcaata
     2041 cattgcagga gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca
     2101 tcgacccgcc tcttttttaa tcgcttcccg gctgttacac tggatagata accagcattc
     2161 ggagtcaaca gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat
     2221 ttatgaatat cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt
     2281 gtatctgttt catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaatat
     2341 ccgcagtcgg gtgctttctc atttacgcac cccggatgaa gccgccatgc tacggcaatc
     2401 ccggcgaatt agctggatct gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg
     2461 attaatcaaa gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg
     2521 tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc
     2581 acgagccccc aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca
     2641 gagcatcgct gatgaacaaa aactttgtta tggtctgttg ggactggaac cgttaagtcg
     2701 cggtcgtgcg tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga
     2761 gagccatgag gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg
     2821 ttggccttgg caaggggcgg tggcgctgaa agaacagcac ccggaaatga ctcaatatca
     2881 tattattcaa aactggctgt ggctgggggc ggttaattcg caggaagaag cgacaacgtt
     2941 aattcggaca cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct
     3001 ttccggtaac tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt
     3061 tcactgaaca acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac
     3121 cagcacgcgc cctggtcggt agtgagcagt ttgtcgccca gttgccacgc cccgttaatc
     3181 acaaacacca ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact
     3241 ttcgccttac agacatccag gcgcgtcata atattgaaat ccatcgacat ctgccctgcc
     3301 gtcagtttcg cttttaccac ctggtccgcc gcaaaggcaa aaggctgtaa cggttttaag
     3361 gtatggttaa agtggtctgc gctttcaagg aacatctcgc cgccttccag caacgtcact
     3421 atcctttcca taccaggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg
     3481 gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg
     3541 gcagcgtttc gccacaaatt caccgacatt ttacgcatat caaagtattc catactcgct
     3601 cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg
     3661 ggtgatttac gttagtggtg atcaggcttt ttttacaact gtttgagaag agaaaagaaa
     3721 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt
     3781 tttttggctg aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc
     3841 tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt
     3901 gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat
     3961 ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg
     4021 cattgcgcga cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat
     4081 ttcgcgaatc tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg
     4141 atgcggaccg aacttgcctt tgtggtgcat catcggcttc gcgtcagccg gtgctgcggt
     4201 agtggtgtct gcggcatggg ccaggttagc cgcgccaaga gccagggtag aggcaacaaa
     4261 cagtgcagtt aatttacgca tattctatat ccttcctttc agttatttat tacggctttc
     4321 ttaagtagcg
//
LOCUS       DBFIFS010000287         4301 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.288, whole genome shotgun sequence.
ACCESSION   DBFIFS010000287 DBFIFS010000000
VERSION     DBFIFS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.288"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..198
                     /locus_tag="N7A07_004460"
     CDS             <1..198
                     /locus_tag="N7A07_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2478987.1"
                     /translation="FNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESV
                     FWLNVKNIPPSIDKKIPMFLR"
     gene            351..500
                     /locus_tag="N7A07_004461"
     CDS             351..500
                     /locus_tag="N7A07_004461"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478988.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            522..3056
                     /locus_tag="N7A07_004462"
     CDS             522..3056
                     /locus_tag="N7A07_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2478989.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            3068..4138
                     /locus_tag="N7A07_004463"
     CDS             3068..4138
                     /locus_tag="N7A07_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478990.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4166..>4301
                     /locus_tag="N7A07_004464"
     CDS             4166..>4301
                     /locus_tag="N7A07_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2478991.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     T"
BASE COUNT         1184 a          977 c         1037 g         1103 t
ORIGIN      
        1 tttaataaag aggataaaag caaaccgcca ttttctgtga tacctcccgt ttctcgcatt
       61 gaaccacagc aagagaaaat attacgcatt ttaaaaacgg ctggcgagtt accaaaagat
      121 cgggagtccg tattctggtt gaacgtgaaa aacatccctc cttcaattga taaaaaaata
      181 ccaatgttct tgagatagcc ataaaaacaa gaattaagtt attctggcga ccagccgcgg
      241 taaagataat ccctgagcag gctattaaaa atgttaaatg gtcgcggcag ggtaataatc
      301 tcatcgtcga aaatccaaat ccgatccata ttaatgtaat gagtgtgacg gtggatgata
      361 aagacgtgtc gctaaatatg atccgccctt ttgaaatatt gacattacca attccggctg
      421 gcgtggcagg aaaatcgctc gtttggcgct ttattaatga ttacggtgca attagtcaac
      481 cactaaagaa aaacctttaa gcgccggata aggatatatc gatggcctct tcgcatcttt
      541 tcattacgct tgcatcgggc atatgtctgc tctgttccat atctgctttt gcccgggata
      601 gcttgttcaa ccccagatta ctggaactgg atcatcctgc ggataatatt gatattcacc
      661 agttcaaccg ttcgaatacc ttacctgcgg gaacatacaa agttgatgtg atgatcaacg
      721 gcatgctctt cgaacgccag gaagttaaat tcgcccagga taaccctgat gctgaactcc
      781 acccatgcta cgtggcgata aaaaacgtgc tggcgaccta tggtataaaa gttgatgcga
      841 taaaatctct ggcgaatgtt gatgacaaaa catgcgtaaa tccagttccg ctgatcgacg
      901 gggctacctg gttactggac gccagtaaac ttgcattgaa tattactatt ccgcaaattt
      961 atctcaacaa tgcagttaat ggttatatca gcccttcccg ttgggatcag gggatcaatg
     1021 ccatgatgat gaattatgat ttttcggcat cgcataccat ccggtcaaat tatgacgacg
     1081 acgatgacag ttattatctg aatttgcgta atggtattaa tttaggcgca tggcgttttc
     1141 gtaattacag caccctgaat tcttatgacg gtaatgtgga ctaccattcc gtcagtaatt
     1201 acattcagcg cgacatcatg gcattacgta gccagattat gattggcgat acctggacgg
     1261 caagcgatgt atttgatagt acacaggtgc gtggcgtgcg gctgtatacc gatgacgata
     1321 tgttgccctc cagccagaac ggctttgcgc cagtggtaca tgggattgcg aaaactaacg
     1381 ccacggtgat catcaaacaa aacggctacg ttatttatca atcagccgta ccacagggcg
     1441 catttgccct caccgactta aacacgacca gtagcggcgg cgatctcgat gtcactatca
     1501 aagaagaaga tggcagcgag cagcacttta ttcagccatt tacttcactg gccattctca
     1561 agcgtgaagg tcagaccgat gtagacctta gcattggaga agtgcgcgac gaaagcggct
     1621 ttacgcctga ggtcttgcag ttacaagcaa tgcacggttt ccctttggga ataactttgt
     1681 atggcggaac acaattggca aatgattacg cttctgccgc gctgggtatt ggtaaagata
     1741 tgggggcgct gggcgcgatt tcttttgacg tgactcatgc ccgctcgcag tttgactacg
     1801 acgataatga gagtggtcaa tcgtatcgtt ttctctattc caaacgtttt gaagacacca
     1861 ataccacctt tcgtctggtg ggttatcgct actctatgga ggggttctac accctcaatg
     1921 aatgggtgtc gcgacaggat aatgattctg atttctgggt aacgggcaac cgtcgcagcc
     1981 gcttcgaagg tacctggacg caatctttca cgccaggctg gggcaatatt tatttaacat
     2041 tcagtcgaca ggaatactgg cagaccgatg aggtcgaacg tttattacag ttcggctata
     2101 acaacaactg gcgaaacatc tcctggaacg tttcctggaa ctatacggac tcgatcaagc
     2161 gctcattggg caaccatcat gatgataaca atgatgattt cggcaaagaa cagattttca
     2221 tgttctcaat gtcgataccg ctatcgtgct ggatggaaga cagctacgtc aactattcgt
     2281 taacgcaaaa caaccaccat gaaagcacga tgcaggtcgg tctgaacgga acgatgctgg
     2341 aagggcgtaa cctgtcttat aacgtacagg aatcgtggat gcactctcct gatgactcct
     2401 acagcggcaa tgccggaatg acctatgacg ggacttatgg ctcggtcaat ggtagctatt
     2461 cctggagccg tgactcccaa cattttgatt atggcgccag aggcggcgtg ctggtgcata
     2521 gtgacggagt gaccttctcg caggaactgg gcgaaacggt ggcattggtc aaagcgccgg
     2581 gcgcagaagg cctgtccatt gaaaacgcca ccgggatttc taccgactgg cgtggttata
     2641 ccgtaaaaac gcagcttagc ccgtatgacg aaaaccgcgt ggcattgaac agcgactatt
     2701 tctccaaagc caatattgaa ctggaaaaca ccgtcatcaa cctggtacca acgcgcggtg
     2761 cggtggtgaa agccgaattt gtcacccatg tcggttatcg cgtgctattt aacgtccgcc
     2821 aggtcaacgg taaaccaata atgtttggcg cgatggcaac cgcctctctc gaaacgggca
     2881 cagtcaccgg gattgtcggt gataacggcg aactgtatct ctccgggatg cctgaaaaag
     2941 gcgagttttt attgagttgg ggacaagctg cggatgaaaa atgtaaggcg gcctatcaca
     3001 tcacccataa acctgatgat accagcctgg ttcaaatgga tgcgatttgt cgctaaagga
     3061 agcatgaatg aaaaaaatat tatcagggtt gattctgctg ctttgctgtc cttatggttt
     3121 cgccgctaat ggtgatggcg caacgcacat gtcaaattta tcatttggtc cgctgacggt
     3181 ggcagcggcg aataaccact ccggatacaa tattttcgag gcactgagca acacgactgg
     3241 aacatacccg gtgcgctgtc actgtgatga cacgcatggc ggaccgggcc aacaaacagc
     3301 attttttcct atcttctaca cgggagatgc cgcaccgggg cttgtgcttg agcgcactct
     3361 taatgggtta aattactatg ctctgaatga ttatttatcg gtcggcgtga cgatttttat
     3421 tattaataac caatatgcgg ccattccttt tgaacactta tccaaccaat ccacctcacc
     3481 gcaacatacc tgtggagcag gtaataatgg gagcactgta aatctggatt cagggcgctc
     3541 ggcaaaatta tctttctatg ttcggcattc tattactggc acggtgacaa tacccacaac
     3601 ggaagtcgcc tggttgtacg cgggcatgtc cgatcatttt cccaaaacga cccccgtttc
     3661 taaagtgaca attcgcggac aactaacggc cccgcagaac tgtgagttaa cgccaaatca
     3721 gagcatcgat gtcgattttc aaaaaattaa tagcgctgag ttctcctcaa cggcgggttc
     3781 aattattgca gaaagaaaga ttaaaaccga agtcacagta tcctgtaccg ggatggaaga
     3841 cgtaaggtcc acggaggtgg tgagtgcgtc gatgattgcg gcaaacagaa gtgccgatgc
     3901 caccatgatc gtgacgagta atccggatgt gggaattaag atttttgata agaacgaccg
     3961 tccagtgaat gtggatgggg gcaacttacc cgctgatatg ggtgctatta gtcgattagg
     4021 aaaaaccgat ggtagcgtaa cgttttattc agcgcccgcc agtctgacgg gcgcaaaacc
     4081 agcgcctgat aatggattta ccgctacagc cacgctggtt attgaattta ctaactaatc
     4141 ttgtgttagt aaaaggaata atttcatgaa aaaatggcat tatatatttt gcataattct
     4201 ctttcattta gggttaccgt gcgggtatgc ggcaaatgat ggcacgtgtg caacaagagg
     4261 cggcacacat acattaagcc ttaattttcc tctgacaacg g
//
LOCUS       DBFIFS010000288         4254 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.289, whole genome shotgun sequence.
ACCESSION   DBFIFS010000288 DBFIFS010000000
VERSION     DBFIFS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.289"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            18..311
                     /gene="bor"
                     /locus_tag="N7A07_004465"
     CDS             18..311
                     /gene="bor"
                     /locus_tag="N7A07_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU2478992.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(402..584)
                     /gene="rzoD"
                     /locus_tag="N7A07_004466"
     CDS             complement(402..584)
                     /gene="rzoD"
                     /locus_tag="N7A07_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU2478993.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(801..1277)
                     /locus_tag="N7A07_004467"
     CDS             complement(801..1277)
                     /locus_tag="N7A07_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU2478994.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(1264..1569)
                     /locus_tag="N7A07_004468"
     CDS             complement(1264..1569)
                     /locus_tag="N7A07_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2478995.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(1891..2580)
                     /locus_tag="N7A07_004469"
     CDS             complement(1891..2580)
                     /locus_tag="N7A07_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2478996.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(2577..2717)
                     /locus_tag="N7A07_004470"
     CDS             complement(2577..2717)
                     /locus_tag="N7A07_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2478997.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(2714..3076)
                     /gene="rusA"
                     /locus_tag="N7A07_004471"
     CDS             complement(2714..3076)
                     /gene="rusA"
                     /locus_tag="N7A07_004471"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU2478998.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(3073..3363)
                     /gene="ybcO"
                     /locus_tag="N7A07_004472"
     CDS             complement(3073..3363)
                     /gene="ybcO"
                     /locus_tag="N7A07_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2478999.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3356..3526)
                     /gene="ninE"
                     /locus_tag="N7A07_004473"
     CDS             complement(3356..3526)
                     /gene="ninE"
                     /locus_tag="N7A07_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2479000.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3526..3981)
                     /gene="ybcN"
                     /locus_tag="N7A07_004474"
     CDS             complement(3526..3981)
                     /gene="ybcN"
                     /locus_tag="N7A07_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HGU2479001.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3978..4076)
                     /locus_tag="N7A07_004475"
     CDS             complement(3978..4076)
                     /locus_tag="N7A07_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479002.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
BASE COUNT         1050 a         1082 c          999 g         1123 t
ORIGIN      
        1 gcatcgggaa taacaccatg aaaaaaatgc tactcgctac tgcgctggcc ctgcttatta
       61 caggatgtgc ccaacagacg tttactgttc aaaacaaacc ggcagcagta acaccaaagg
      121 aaaccatcac ccaccatttc ttcgtctctg gaattgggca gaagaaaact gtcgatgcag
      181 ctaaaatttg tggcggcgca gaaaatgttg ttaaaacaga aacccagcaa acattcgtaa
      241 atggattgct cggttttatt actttaggca tttatactcc gctggaagcg cgtgtgtatt
      301 gctcacaata attgcatgag ctgcccatcg atatgggcag ctctatctgc actgctcatt
      361 aatatacttc tgggttcctt ccagttgttt ttgcatagtg atcagcctct ctctgagggt
      421 gaaataatcc cgttcagcgg tgtctgccag tcggggggag gctgcattat ccacgccgga
      481 ggcggtggtg gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc
      541 agcggcaaca tcatcatgca gagcatcatt ttcagctttc gcatcagcta actccttcgt
      601 gtattttgca tcgagcgcag caacatcacg ctgacgcatc tgcatgtcag taattgccac
      661 gttcgccagc ttcagttctc tggcattttt gtcgcgctgg gctttgtagg taatggcgtt
      721 atcacggtaa tgattaacag cccatgacag gcagacgatg atgcagatga gcagagcgga
      781 gataatcgcg gttattctgc tcatacctca ctctctctga ccgttccgcc agcttctttg
      841 aattttgcaa tcaggctgtc agccttatgc tcgaactgac cataaccagc gcccggcagt
      901 gaagcccaga tattactgca acggtcgatt gcctgacgga tatcaccgcg atcaatcata
      961 ggtaaagcac cacgctcttt aatctgctgc aatgccactg cgtcctggct tttgggggag
     1021 aagtctttca aaccaagctg tttacggtaa gcatcccacc agcgtgaaag aagctgataa
     1081 cgtccggcgg ctgttgattt gagtttgggg tgtagcgtga caagtttgcg agggtgatcg
     1141 gagtaatcag taaacagttc gccgccaaca ataacatcat aaccgtggtt acgtgtcggt
     1201 tgtcgcccgt tatccgttcc ttctgaccac gccaacatat cgaggaaggc tttacgctga
     1261 ggattaagat tttgcatttt tcacccctgt cagtcgttcc cagaagtacg tcagtgcaac
     1321 cgaacccatc gcaccactaa tccccgctgt cgcgagaatc atgtaaatac tgaatccact
     1381 ttcgatgctg atcaggccac caataacacc ggtgaatcct gataccacta tttgagccag
     1441 agcatttatc caactccacg ttgctttact ctgcttcaca tctatcaggt agcggaccag
     1501 accgccccaa cctgcgatga tcagcaaaac gagccagaac gctccggcaa ggctctcttt
     1561 ttcgtgcata tgaatagcca atgtttcgcc gccgacaaaa ggccgggacg ttaaatgtca
     1621 gaaatcaggc tcacggggta atttaacgac aaagcacgga gttgatgctc cccacaagcc
     1681 tggaataaaa aagccagcat gtagctggca acagagggct aagcaatatc aactcaacag
     1741 ctgaagaccc cctggctggg gtaggttgga aggctactca ccgttcagaa acagaaaagc
     1801 ccaaggcttt aaacctcggg cttgaatttg gattactgcc agtgcgtaca acattggcaa
     1861 aatatcagat ttacataaaa tatatgattt ttaatccagt tttgcaatat cttgctgtga
     1921 aaatgtggtc ttttgttttg aacgtgtttt cgttaaaagc aataaagctt ggctatcaag
     1981 ctgtagaaaa atgtgtttca ttgcaaccca gcgttcagta aatgtctcag accagttttt
     2041 tgatgtcact cccaccagtg atgccagctc ctggtattca taggtcttac gccctgccag
     2101 ctcgttcttc acatcctgtg ccgccagcca gatcaacttc ttcaaacgtt ccagtgtctt
     2161 acctgcaatt ttcctggtac ccaacagagt cttaaactcg ctccatgccc actgcgttat
     2221 ggtgacctga tgttcccagc gaacactttc gctgtaactc cacagcaacc acgctttctg
     2281 atgttcttcg agagacagaa ccgcgcggcg ccatgacgag gttgagaact caacctggct
     2341 gaccagtgca atggatgaac cttttgcgta cgactgctta ccggaaatcg gcggattatc
     2401 cagcgtaatc atcctgccag ttacctcatc cagaatgcgc ggcttctttc gtttgtatgt
     2461 accagtatca aattgtgcat gctccagcca ggcttcaagc tgtcctttcg ttgctccgct
     2521 caaatcagcg gtagccacaa tgagttgctc gcggacatac tgtaaatatt gggtattcat
     2581 gcggcagctc ctttcagtgt tttggcgtaa ttcttcagta tccggtaatc ggtcaaaaca
     2641 gaaccaggaa aacgatataa gcgcaggcgc atccagcggt ggcgaagacg ttctgccata
     2701 taagactcaa acatcattca ttccccagtt cggtgatggt cagctccagc ttcccacctt
     2761 tggtaacggg catcttcaca acacggtaat caacgacctg agcatcatcc agccagaaac
     2821 ctgctttggt gagtgcgtca aaagcggctt tttgcagatt atccaggtca cggcgacggc
     2881 gatccggcat gtggcactca atacggattt tcactggcat agccaggccg atatccagca
     2941 tggagccttt gatgattcgg gcgacgttat cacggtatgc ctgcccttct gcgctgatgt
     3001 gcgtgcgccc gcgattatgc cggtagtagc gattattgct cggaggccag ggtaatgtga
     3061 tgctgtaggt attcacgcct taataacccc ctctttcagc cagataacct gtgttctcgc
     3121 cataccttcc agcgcgcatt cttttgcata tgcagcatcg acaaaatgtg tgcggcggtc
     3181 gatttcgtcg tggcaggcag aacatgcaat ggtggcaatc aagtctggcg gtttggtacc
     3241 ggtgccgcac aatccagtca gccggatatg tgccagtaca gacgtttcag ggttgccatt
     3301 acatacgcca gggattctta cttggcattc ccgactacgc gctgcttttc tcaaatcagc
     3361 catgattcct ccttgctgcc agtcgcaacc attttttatc aaccaggctg gcggtatatc
     3421 cgagcagtgt tggtatttcg gatggcttca gctcaggttt acgcttacga cgatttggta
     3481 ctttgtagat gtgtccgttc atgacacgaa taagcggtgt agccattacg cctcctgctt
     3541 gtcacgcagc agctggaact cgcagctctg tggaatagtc aggtggcagc caatattcat
     3601 cgcccaggct tcaaccttac acaggaagac atacatctct ccggtaccaa gatcggaggt
     3661 atggcgtaac gactggatag tagtgatttc gccggttacg acatcaacca ggtccttggt
     3721 ttcataaccg aggtatgtgt gtttgagagc atcttttacc catgctgaag tagcgaacga
     3781 tttccccctg ctgatgaggt attcactgat ttcgctgtac cacatgtggc tgagtgcatt
     3841 ctgggaaaga ctgcgtctct cgcgccacgg tttaagcacc atgcgaaagc atttgccgtc
     3901 ttccagataa gtctggatct gctgaccgac agcggtgaag ttaccgcgat gtaatttgat
     3961 gccatcttgt gggaggttca cgcttcacct ccgcagagat caaacgctgg atgcaagaca
     4021 tcgcaggtgc atttctgcat ctgtgacaag gtaaggagtt cagattgtgg tcgcatttaa
     4081 gtccccttaa atgcgcagaa gtcaccggag ttgttcaagc tccggtgaca ttattatgga
     4141 aggttgatac taaaaaatca aaaattgaat atcattggcg atagtctagc ttgtgatatc
     4201 cacaagctac ttcaatgaag cgacgagtaa cattttgtgt catccatgtt tttt
//
LOCUS       DBFIFS010000289         4221 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.290, whole genome shotgun sequence.
ACCESSION   DBFIFS010000289 DBFIFS010000000
VERSION     DBFIFS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.290"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            227..835
                     /locus_tag="N7A07_004476"
     CDS             227..835
                     /locus_tag="N7A07_004476"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU2479003.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="N7A07_004477"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="N7A07_004477"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU2479004.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..4165
                     /gene="selB"
                     /locus_tag="N7A07_004478"
     CDS             2321..4165
                     /gene="selB"
                     /locus_tag="N7A07_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU2479005.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          975 a         1068 c         1211 g          967 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtgacgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     1381 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc ggaaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt agacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggttctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg
     2821 cgagcacgcc agccaacata gtttccgcct cgcgattgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gccaatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact
     3241 ggtcttcgac cccccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc
     3301 tgcccgcaac acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaaacca gagtatctgc aatgcctggc ttccctcgcc agggcacaga atgatagcga
     3421 agcgttagcc gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcattatta tttccggaaa aataaggaaa tgattaatta atttttaaaa taaattaata
     4201 caaatttctt atgattttaa a
//
LOCUS       DBFIFS010000290         4208 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.291, whole genome shotgun sequence.
ACCESSION   DBFIFS010000290 DBFIFS010000000
VERSION     DBFIFS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.291"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1033
                     /locus_tag="N7A07_004479"
     CDS             <1..1033
                     /locus_tag="N7A07_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU2479006.1"
                     /translation="RNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTVDSGEASL
                     SLVITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAEKVLPADFVKS
                     TVHKFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGND
                     VFERHAGHIAALIRYIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSH
                     FKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDF
                     VVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKL
                     GAFLSGLTG"
     gene            1534..2766
                     /locus_tag="N7A07_004480"
     CDS             1534..2766
                     /locus_tag="N7A07_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2479007.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            2751..3389
                     /locus_tag="N7A07_004481"
     CDS             2751..3389
                     /locus_tag="N7A07_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGU2479008.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            3468..3737
                     /locus_tag="N7A07_004482"
     CDS             3468..3737
                     /locus_tag="N7A07_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU2479009.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            3758..>4208
                     /locus_tag="N7A07_004483"
     CDS             3758..>4208
                     /locus_tag="N7A07_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479010.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYD"
BASE COUNT         1121 a          852 c         1124 g         1111 t
ORIGIN      
        1 caggaacatg gtgccagtaa cccttcttaa agagcattat cgttgtcatc ctaaaatcat
       61 tcaattttgt aataagcagt tttatgataa tgctctgata ccgttaacgg ttgattccgg
      121 tgaagcctca ttatctctgg tcatcacggc aaaaggaaac catacccgta atttttcaaa
      181 tctcagggag cttgaatctc tggaaggtca ttactgggat gaagaaagta gcaggggata
      241 cattgcccca tataatgcac aggttaatct ggctgaaaaa gtgctgcctg ctgattttgt
      301 gaaatcgacg gtgcataaat ttcagggacg ggagtgcgat gaaattgtgt tttccactgt
      361 gctggataag aagcgcagca gccagcacag tcggaacatc gcgtttgttg ataatcctga
      421 actggtgaat gtcgctgttt caagggcccg aaataaattt acgctggtga ctggtaatga
      481 tgtatttgaa cgccacgctg gacatattgc ggcgctgatt cgttacatca aatactacgc
      541 tgatgacgga gagattttcg agagcccggt catatcagca tttgatttgc tgtacagtga
      601 gtatgacaaa tcactggaac gactgaatag tagattgaat tcaaatgatt cacactttaa
      661 gtcagaacag attgtggcct gcctgttgcg ggatatcctg tcgcaggata gttatcggag
      721 tatgatgttt cattcacaaa tagcgctgaa tcagttggtg ttgctggagc ggggggattt
      781 tacacaccgt gaacagttat tcatgcgtaa ccgggccagt tgtgactttg ttgtttacta
      841 taaggtcggg aagactccat taggggttat tgaagtggat ggaggttatc atctgaccag
      901 tgttcaggct gaacgtgatg aacttaaaaa cagtatactg aagaaatgcg gcctgccatt
      961 gctaagatta cgtactatag acagtgatat agaagggaag ttgggagcgt ttctgtcagg
     1021 cctgacaggc taaaatctga ataaaaaata ttgcagcggg aggtctcaaa cctgatttgg
     1081 tcatgtgcca cacacaattg tacgaacccc atggacacag gcttctttgt tttggttaca
     1141 tcaacgtacg tattcctgat acatataaaa ataagtgcta ttgctcaaag ctaacaggga
     1201 atgtttctgt tgaataattc cttagtgaat aaactttatc agggggtaat tctattacag
     1261 tgaatacttc ctgcaaaatg atatttgaat tgttcaccat ctgcatcata ttttcttatt
     1321 tacaagttga aaggatagta tatgttgaaa atgcatggat tttccaggtg tgttaccagt
     1381 aagaagcagg tggataaagg atgtgataac aaatccaaca accaggttaa tatctgaaga
     1441 tttttgttgt tattatttat ccccaaagcg gtgagttatt aatgaagttt gcctgaatgt
     1501 ataaacagaa ttattcgaca taaagccagg aaaatgagtg atacgctttt aaccgaaaaa
     1561 atccttaccg gagaaaatgt tttgcgtgct gcaatcgccc gcattgagtg gatttttgaa
     1621 acatttccgt cggtatgtct gtctttttca ggtggaaagg attccaccgt gcttttccat
     1681 cttgtggcag aggtggcccg caggagaaaa cgtcatttct ctgttctgtt cattgactgg
     1741 gaggcccagt atcggtgcac cattgaacat attcagaaga tgcgggaaat gtaccatgat
     1801 gtgacggaaa ccttttactg ggtggcactt cccctgacca cggtaaacgg cgtctcgcag
     1861 tttcagccgg agtggatatg ctgggagcca ggagtgacct gggttcgcca gccaccggaa
     1921 gaggccatta cggatatgac atattttccc tttttccggt atgccatgac gtttgaagaa
     1981 tttgttccgg cattttcttc ctggtttgcc ggtaaccggt gtggagtggc agtgctgacc
     2041 ggtgttcgtg cggatgaatc cctcaatcgc tttatggggc tggtgtctca gcgcaaactg
     2101 agatatgccg atgataagcc ctggaccacg gcatcgcctg aagggtttta ttacaccatg
     2161 tatccgttgt atgactggaa agcccgcgat atctggatat ataacgccag agcttgtgcc
     2221 atttacaatc ctctgtatga cctgatgtac cgtgccgacg tgccgttacg caacatgcgt
     2281 gtctgtgaac cttttggtcc tgaacaacgt aaggggctgt ggctttacca tgttctggag
     2341 ccggaaacct gggccaggat gtgtgagcgg gtgtcaggcg ctgccagcgg ggcgctttat
     2401 gccaatgaaa gcggtgccta ttttgccctg cgtaaacgta tatcaaaacc agcccatcat
     2461 acctggcgca gctatgcgat gttcctgctg gatgtgatgc cggaaagaac ggcagaacat
     2521 taccgtaata aaattgctgt ctacctgcgc tggtatcaga cgcggggctt cccggatgac
     2581 atcccggatg aacaggagaa tgacctgggg agccgggata ttccgtcctg gcggcgtatc
     2641 tgtaagacac ttataaagaa tgatttctgg tgccggcccc tctccttcag tccgaacaaa
     2701 ccccggcact atgaacgtta tctgcagcgt atgaaagaaa ggaggaagga atgggggatt
     2761 ctgtgacacc ggagataatt ggcaacatga ttcgtcagta cttcagccag gaacgaccag
     2821 aagaagagac catacaggcc cttaatcacc tgcgcagagt gcttcatgaa gtcagtcctt
     2881 ttgcacatga gccagtggac tgtgtgctgt gggtaaaagc agatgaggtg gtggccaatg
     2941 attacaatcc taatgtcatg gcgccgggtg agaagaaact gctgaagcag tcactggaga
     3001 aagatggttt cacacaaccg gtggtggtgt ctgaggaaaa gaaccattac ctggtggtgg
     3061 atggttttca tcgccaggtg ctgggcaggg aagcaggtac gggaaaacgt ctgaagggct
     3121 ggctgccggt ggtctgtatt aatccggaga gaaagggaca ggcctcacgt attgctgcga
     3181 ccatccgaca taaccgggcc aggggcaaac atcagataac ctcaatgtct gatattgtca
     3241 gggacctgtc gcgcctgggc tggacgaatg aacgcattgg tacggagctg ggtatggacc
     3301 aggatgaagt tctcagattg aaacagatta gcgggctgac ggagctgttt caggaagagg
     3361 atttcagccc ggcatggacg gttcgctgag accgtcagat atgtatatta tttcagtgag
     3421 ttaatgtttt gagcaggaaa aagagtaccc cggaagttgt ggaagacatg gttgcgcaga
     3481 aactggaggc tgctggttgc tggcgcagag cttctgaccg ctggcttttt gttatgggaa
     3541 atgttgaatg tacagaagca caacgggagt ggttgttgtt gcgccggaat tattgtctgg
     3601 cgcagatatc gtccccgccg ctacctgaaa cgctggatat cagcgaggtg gcgaaagctg
     3661 cagatgccac gctgaggcga atggggattg ccactccgtc cggggaggta ttcagaaaag
     3721 gtacaccggt ctgttgatat tcaggtaaca ggtattaatg atgaagcaag gaatggcaaa
     3781 cagtatatca atgacatggc aacttttatg cgatgaacat ggttttaccc gggagcacta
     3841 caatcagctg aagaagttca gcccggagac attacgtgaa attattgctg aaatagccag
     3901 ctgtcatcca tcaaccagtg tattgctcag gaataagtgg ctgacaccgc cggaggatat
     3961 tctggagcag attacccgtg aatacgaacg aagaatacaa aactgtccac cgttcaggag
     4021 cgagaaggaa gcggaatcct ggttaaatga actatctttt gctgtgattg ctccgctata
     4081 ccgtgttgcc cccgaacaaa cagaacaggt tgagtccttt gttctgcagc ttattgccga
     4141 gcaggagcgg gtttgggagc ttattttaac caatgatggt tattcatggc attgcgcact
     4201 ttatgata
//
LOCUS       DBFIFS010000291         4153 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.292, whole genome shotgun sequence.
ACCESSION   DBFIFS010000291 DBFIFS010000000
VERSION     DBFIFS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.292"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..916
                     /locus_tag="N7A07_004484"
     CDS             <1..916
                     /locus_tag="N7A07_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU2479011.1"
                     /translation="KSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTP
                     SAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDK
                     QRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMF
                     CTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLRQRI
                     QEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQ
                     IDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1125..2090
                     /gene="lipA"
                     /locus_tag="N7A07_004485"
     CDS             1125..2090
                     /gene="lipA"
                     /locus_tag="N7A07_004485"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU2479012.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2191..2394)
                     /gene="tatE"
                     /locus_tag="N7A07_004486"
     CDS             complement(2191..2394)
                     /gene="tatE"
                     /locus_tag="N7A07_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU2479013.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2523..3311)
                     /locus_tag="N7A07_004487"
     CDS             complement(2523..3311)
                     /locus_tag="N7A07_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU2479014.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3404..3787
                     /gene="crcB"
                     /locus_tag="N7A07_004488"
     CDS             3404..3787
                     /gene="crcB"
                     /locus_tag="N7A07_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU2479015.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3841..4050)
                     /gene="cspE"
                     /locus_tag="N7A07_004489"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3841..4050)
                     /gene="cspE"
                     /locus_tag="N7A07_004489"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU2479016.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1085 a         1070 c          949 g         1049 t
ORIGIN      
        1 aaaatcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaatct
       61 tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct
      121 taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga
      181 cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca
      241 tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta
      301 tgataagcaa cgaacgataa ctattgcgac caccccctcg gtcggagctc tggtccttcc
      361 tgtcatttat cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat
      421 cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatag ataacatgtt
      481 ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg
      541 ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc
      601 acatgtactc ctgttaccgg aaggacaaaa tttcagcggt ctgcggcaga gaattcaaga
      661 gatgtttccg gaccggcaga tcagcttcac cagctacaac attttgacaa tcgctgcact
      721 ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagccg
      781 ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc
      841 tatccactac aacaaattca gcctgcgtga tcccatcctg cacggagtaa tagatgttat
      901 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
      961 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
     1021 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
     1081 ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg
     1141 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
     1201 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
     1261 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
     1321 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa
     1381 cgtttatgat cctgggcgct atttgtaccc gccgttgtcc gttctgtgac gttgcccacg
     1441 gtcgcccggt agctcctgat gccaatgagc cagtgaaact ggcgcagacc attgccgata
     1501 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
     1561 cccaacactt tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
     1621 aaactctggt gccggatttc cgcggtcgta tggatcgtgc tctggatatt ctgactgcaa
     1681 cgcccccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac
     1741 ggcctggcgc agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc
     1801 cggaaatccc gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta
     1861 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt
     1921 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
     1981 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
     2041 gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa tcctgtaata
     2101 attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca
     2161 agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg
     2221 aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa
     2281 ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag
     2341 cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac
     2401 cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccaaagaata ttaacggtca
     2461 ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa
     2521 catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag ttgcgcgcgc
     2581 acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct
     2641 gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg
     2701 cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt
     2761 gaccagtggt gctctttgag cggcccgcga acccaggcgg caggaagtac caggatttca
     2821 gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc
     2881 agaccgacct tcatcccctc cacctcgagt aacggagcaa tttcattacc agcatcaaca
     2941 cggcgtgatt cctgaatggc aaatgcatca tagagatgca atttggcata acgggcgacg
     3001 atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt tgaaggaaca
     3061 tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg
     3121 aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc
     3181 gccagcaatg cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc
     3241 aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca
     3301 gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc
     3361 gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt
     3421 agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa gtatgcgatt
     3481 taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt
     3541 catcatagga atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa
     3601 agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga
     3661 agtggtgttt ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa
     3721 ccttctgggg tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgc
     3781 acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc
     3841 ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc
     3901 tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac
     3961 gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa
     4021 ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac
     4081 aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtccagt gcaactttac
     4141 taaaaagtga taa
//
LOCUS       DBFIFS010000292         4134 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.293, whole genome shotgun sequence.
ACCESSION   DBFIFS010000292 DBFIFS010000000
VERSION     DBFIFS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.293"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..487
                     /locus_tag="N7A07_004490"
     CDS             <1..487
                     /locus_tag="N7A07_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGU2479017.1"
                     /translation="AADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKR
                     QTDKLVMGMGSTTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKL
                     VQLLGDVPRIELFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGIT
                     S"
     gene            447..659
                     /locus_tag="N7A07_004491"
     CDS             447..659
                     /locus_tag="N7A07_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2479018.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADTELH
                     LFGEILSRKLDELEKLVEEADTSPIA"
     gene            695..1072
                     /locus_tag="N7A07_004492"
     CDS             695..1072
                     /locus_tag="N7A07_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2479019.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQPVQNNIIGDLLRKSRKEARRAGRKQKR
                     WEGACKALQELNKYRDLINELSE"
     gene            1151..1333
                     /locus_tag="N7A07_004493"
     CDS             1151..1333
                     /locus_tag="N7A07_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2479020.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(1317..2486)
                     /locus_tag="N7A07_004494"
     CDS             complement(1317..2486)
                     /locus_tag="N7A07_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2479021.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPVFTQ
                     VKPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            2682..2756
                     /locus_tag="N7A07_004495"
     tRNA            2682..2756
                     /locus_tag="N7A07_004495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2715..2717,aa:Arg,seq:cct)
     gene            2918..4075
                     /gene="intS"
                     /locus_tag="N7A07_004496"
     CDS             2918..4075
                     /gene="intS"
                     /locus_tag="N7A07_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU2479022.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
BASE COUNT         1234 a          938 c          952 g         1010 t
ORIGIN      
        1 ggcagccgat aattgcctgt tggctatgtg gtgggtgcct acacaaccac ttgaagcatt
       61 gaaggttgta gaagcgtggg gctttcgtct ggtgacgatg aaaggattaa cctggaacaa
      121 atgcgggaaa agacagaccg acaagctggt catgggtatg ggtagcacca ctcgcgctaa
      181 cagcgaagac tgcctttttg cagtgaaagg aaatctgcct gaacgcatta acgccggaat
      241 aatccagtca ttcactgcac cgcgccttga tcactcccgc aagccggata tggcgcgaga
      301 aaaacttgtg caacttcttg gcgatgttcc ccggatagaa ctgttcgccc gtcacacctc
      361 gcatggattt gatgtctggg gtaaccaatg cggaacacca tccattgaga tggttccggg
      421 tattgttaaa tttctggaga aaaccaatga gcgaaaaaac gacgttgaca aaggcatcac
      481 cagttgaatt aaggcagtgc ctggaaatcg caaatcaact tgccagaagt ggaatacgct
      541 ttgttccgat cccgatcaca gcagatacag aacttcatct gtttggtgaa attctttccc
      601 gaaagctgga tgaactggaa aagctggtag aagaagctga cacctcacca atcgcataac
      661 acccccaccg acattaaaat atcaggagaa aaaaatgaac gccgtactca cagaattgaa
      721 caaattagga aaagcaacag ccgacagcat ttctaaaggt ctcaatattg atttgaatga
      781 cgtcattgat acgctgtgga agttgaaaaa ccaggggatc gtaaaggtaa aaaacggcat
      841 ctggcaggca gtagagggag ttggaagcaa aacagatatt gattcagttc aaccagttca
      901 accagttcag ccagttcagc cagtgcagaa caacattata ggtgacctgc tacgtaaatc
      961 acggaaagaa gcgcgccgcg ccgggcggaa acagaaacga tgggagggtg catgtaaggc
     1021 gttgcaagaa ctgaataaat accgtgactt gatcaacgaa ttgtcagagt gaacgtagcg
     1081 ggaatccacg tcctttaggg cgtggatgat atcaggctgt tgaaatacat gtagaaaagg
     1141 agcgcatact atggaagtca acttaatgac cgaaaaagag gtttctgatt tgttacaaaa
     1201 aaggcgcaca gctttgtata atttgcgaaa aaaacacgga ttcccggaac cagtacttac
     1261 tcatccggcg cgatatagtc gtcaggccgt tgaacaatgg cttaaagccg gaggaattaa
     1321 ccgagctgtt tagcgtgcca gaaaatttta tcagcataca actcatacgc atctttctgt
     1381 tccgacaacc agtcgtgttt attatacacg gccataactc ccccaagttc atgccccagc
     1441 attttttcag tgacgtgggg cataattcct tccccagata aattcgttac cagagaacgc
     1501 ctaaaatcat gcgttctcca ttcaggaata tcaattttct cccttaactt tttcatgtac
     1561 aaattagccg acgaacgatc aataggttta tctatttcct gcccggggaa tagcacatca
     1621 aaacccgcat taagcaatct ctcgatataa ggtttaacct gagtgaatac cggacgccgg
     1681 ataatattcc ccatttttga atgctctttt ggggttgtcc agaccagatc ttccatgttg
     1741 aactcactgg ctgtcgcgag acgtagctca gaaagtctgg ccccccatag caatagcaat
     1801 tgatgtaaca ccttgttgga agtaactact tttgagttct ccagcgccag ccaaattttt
     1861 gccagttccg tataggtaag tacacgactc cctacatctg gttttttccc aatattctta
     1921 acactaagct tgagtaattc gcatgatggg attaactggc ggctgataca ccagttaata
     1981 actgaacgca gttgtaacaa caaaacacga gcctttttct tgttgaggct ttcttgtttg
     2041 tcaaagaaac gagcccatgc cgatactggg atactggcaa ctggcgagcc ttcaaactgt
     2101 gtgtacatag tgttgtacac gacggatctg taaagcgtct gtgtattggg tttcagatca
     2161 gtaacatact tatcccacca ctgatccagg cattctttta atgtcagttc gccgtcactg
     2221 ggcgcaaaat aatttttagg gttaactccc ttcatgtaca atgcgcgcat ctcaccgacg
     2281 ataacacgcg cgtccttcag tgaagtagac ggatagcgcc cgacagtaag acgtactggc
     2341 ttaccattcc agcgataacg gtactgaaac gtgatagttc ctgctggagt aatccgcaca
     2401 cttaacccgt caccatcggt gatttcagca gggccagagt agggttttcc gtagaggcct
     2461 cgtagtttgg tgtcgctcag tgccaatgct ttatatcctg tacacaagtt tataatgtat
     2521 tctgtactca atatgtacgc attagcaagt gaacaaaaca cttttcaacg caaaagaaga
     2581 taatcaacca tgaacaaaaa caaacttaca ccttgatttt aaaaccataa agacggtatt
     2641 atcagtcata tggtgaacaa ccaaaatcaa tacgcaacaa tgtcctctta gttaaatgga
     2701 tataacgagc ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta
     2761 tcagttcgcc tccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtaga
     2821 ttcacagttc gtcatggttc gcgtcagatc gttgacagcc gcacgccatg acgggtaaaa
     2881 agtggataaa ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga
     2941 agcagcaaag ccgaaagaaa aaccataccg cctactcgat ggtaatggcc tgtaccttta
     3001 tgtccctgta tcagggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa
     3061 aatcctgacc gtcggaaaat atccgcttat gactttgcaa gaggcaaggg ataaagcatg
     3121 gactgcgagg aaagacatct cggttggcat cgatccggta aaggcgaaaa aggcttcgtc
     3181 taacaacaat tcctttagtg cgatttacaa ggaatggtac gagcataaga ggcaagtctg
     3241 gtcagccgcc tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacctatcat
     3301 cggcggcctt gaaattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt
     3361 tgaagatcgc ggggcaatgg agcgagccaa caaagcacgc agaagatgcg gcgaggtttt
     3421 ccgttacgct attgtcaccg gaagggctaa atataacccg gcacctgacc ttgctgaagc
     3481 catgaaggga taccgcaaga agaacttccc gtttttacct gccgaccaga tcccggcatt
     3541 caacaaagca cttgcaacat tttcaggaag tatcgtatct ctcattgcga ccaaagtttt
     3601 acgctacaca gcactaagaa cgaaagagct tcgttccatg caatggaaga acgtcgattt
     3661 tgaaaacagg attatcacca tcgaggccag tgtgatgaag ggacgcaaga ttcatgtggt
     3721 tccgatgtcg gaccaggttg ttgaacttct cactacgcta agctccatca ctaaaccagt
     3781 atcagagttt gtttttgccg ggcgcaacga taagaagaag tcaatctgtg agaacgctgt
     3841 actgcttgtg atcaaacaaa tcggctatga aggtctggaa agcggtcacg gattcaggca
     3901 tgaattcagc acgattatga acgagcacga atggcctgct gacgctattg aagtgcaact
     3961 agcacatgcc aacggcggat ctgtgcgtgg gatttacaac catgctcagt atctcgataa
     4021 gcgcagagaa atgatgcagt ggtgggcgga ctggcttgat gggaaggtgg agtagttatt
     4081 acaacgattc ttataaaaaa gaaaagcctt gcacatatag caaagctttt cact
//
LOCUS       DBFIFS010000293         4114 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.294, whole genome shotgun sequence.
ACCESSION   DBFIFS010000293 DBFIFS010000000
VERSION     DBFIFS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.294"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(23..268)
                     /locus_tag="N7A07_004497"
                     /pseudo
     CDS             complement(23..268)
                     /locus_tag="N7A07_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(275..526)
                     /locus_tag="N7A07_004498"
                     /pseudo
     CDS             complement(275..526)
                     /locus_tag="N7A07_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            583..1971
                     /locus_tag="N7A07_004499"
                     /pseudo
     CDS             583..1971
                     /locus_tag="N7A07_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            1991..2737
                     /gene="traX"
                     /locus_tag="N7A07_004500"
     CDS             1991..2737
                     /gene="traX"
                     /locus_tag="N7A07_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU2479023.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2796..3656
                     /locus_tag="N7A07_004501"
     CDS             2796..3656
                     /locus_tag="N7A07_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2479024.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3759..>4114
                     /locus_tag="N7A07_004502"
     CDS             3759..>4114
                     /locus_tag="N7A07_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU2479025.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHK"
BASE COUNT          956 a          983 c         1269 g          906 t
ORIGIN      
        1 aaccagaagt gggcgggaga catcacgtat tgtgtgcccg gagttcaggg cgagcatgga
       61 ggctcaaatg aaccacgagt ctgtctggaa tattgaaccg gtaactctct cctttgatat
      121 tcagcgtggt tgagtgatgt agcagccggt ccaggatcgc tgttgccagc acatgctctc
      181 cgaacatttc tccccagtct gcgaaccctt tgtttgacgt cagtatgatg ctcgcttttt
      241 catatcgacg gttcagtagc cggaagaact gaacgcgcag ttcacgattt tcacgttcca
      301 gctctttcag acgctgacgt tcagcggtgg tgagtccacc atcaccactc ccggtatccc
      361 gctcatgctg acgaacccac acacgcagag tctctggtgt acagccaatc tttggggcaa
      421 tggaacaaat tgccgcccat tgtgagtcat attcgccctg actttccaga accatacgaa
      481 ctgcccgttg acggacctcg ggggaaaaac gagtattttt agtcatcctg tttacctctt
      541 tctcagggag tttagtctcc aggatttccg gggcggttca atcaccggat tgccggtatc
      601 agcagggatg acgggctgat aactctgcag gatgcggaag gtaacacgcg gctgatttcg
      661 ccccgggagg cggtggctga aggcgtcaca ctgtaccccc cggacaccat cagggtgggg
      721 accggtgacc ggatgcgctt cacgaagagt gaccgggggc gtggttatgt ggccaacagc
      781 gtctggacgg tgacagcggt ttccggtgac agtgtcacgc tgtcgggcgg acagcagacc
      841 cgggtgattc gccccgccca ggagcgggca gagcaacata ttgacctggc ctatgccatc
      901 accgctcacg gtgcgcaggg ggcaagtgaa acctttgcca tcgcgcttga aggtacggaa
      961 ggcagccgga aactgatggc cggctttgag tcagcctacg tggccctgtc gcgtatgaag
     1021 cagcatgtgc aggtgtacac cgataaccgt cagggctgga cggatgccat taacaatgcc
     1081 gtacagaaag gaacagccca cgatgtgttt gaaccgaaac cggaccggga ggtcatgaat
     1141 gcagagcggc tgttcagtac ggcgcgggag ctgcgggacg tggcggcagg gcgtgccgtt
     1201 cttcgtcagg cggggcttgc cgggggagac agtcctgcac ggtttattgc tccgggacgt
     1261 aaatatccac agccgtatgt ggcactgccg gcgtttgacc gtaacggcaa gtctgccggt
     1321 atctggctga acccgctgac cacggatgac ggaaacgggc tgcggggatt cagtggtgaa
     1381 ggacgggtga aaggcagcgg ggatgcgcag ttcgtggccc tgcagggcag ccgtaacgga
     1441 gagagcctgc tggctgataa tatgcaggat ggtgtccgga ttgcccgtga taatcctgac
     1501 agcggtgtgg tggtgagaat cgccggtgaa ggtcgtccgt ggaatcccgg tgccatcacc
     1561 ggtggtcgcg tgtgggggga tatcccggac aacagcgtcc agccaggagc cggaaatggc
     1621 gagccggtca cggcagaggt actggcacag cggcaggctg aagaggccat ccgccgtgaa
     1681 acggaacgcc gcgcagatga aattgtccgt aaaatggcag agaacaaacc tgacctgccg
     1741 gacggcagaa cagagcaggc tgtcagggag attgccgggc aggagcgtga acgggctgtc
     1801 acttctgaac gggaagccgc gctgccggag agtgtactgc gtgaaccaca acgggagcgg
     1861 gaggcggtcc gtgaggttgt ccgggaaaac ctgctgcagg agcgactgca gcagatggag
     1921 cgggatatgg ttcgtgacct gcagaaagag aaaaccctgg gcggagactg atacaggaag
     1981 acaaacactg atgacaacgg ataacacgaa cacgacacgt aacgattcac tggctgcccg
     2041 gaccgatacc tggttgcagt cgttgctggt ctggtcaccc ggacagcggg atatcatcaa
     2101 aacggtggca ctggtgctga tggtactgga ccacgcaaac cgcattctgc atctggacca
     2161 gacgtggatg tttctggccg ggcgtggagc ctttccgttg tttgctctgg tgtgggggct
     2221 gaatctgtcc cgccatgcgc atatcaggca gtcggccatt aaccggctgt ggggatgggg
     2281 aattattgcc cagttcgcgt attatctggc aggctttccg tggtatgagg ggaatatcct
     2341 gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt gaaacgcgca gtgggtggcg
     2401 tacagcagcg gccattctcc tgatggcact gtgggggcct ttgtccggca ccagttacgg
     2461 cattgccggg ctgctgatgc tggcagtcag ccaccggctg taccgggcgg aagacagaac
     2521 ggaacgtctg gtgctggttg catgcctgct ggccgtgatt ccggcactta accttgcctc
     2581 cagtgatgcg gcggctgtag ctggtcttgt gatgacggtg ctgaccgttg gtctggtgtc
     2641 ctgtgcaggg aaatcattac cccgtttctg gcccggagat tttttcccga cgttctatgg
     2701 ctgtcatctg gctgtgctgg gcattctggc gttgtgactg gtatgatact gacgctgatt
     2761 ctgcttatta ataaacaaca taacacagga tatttatgaa aattacggat cacaaactct
     2821 ctgaaggtat tgccctgaca tttcgtgttc ctgaaggaaa tataaaacat cctttgatta
     2881 tcttatgcca tgggttttgc ggtatcagga atgttctgct cccttgcttt gcgaatgcct
     2941 tcacagaagc aggattcgca acgattactt ttgattatcg aggattcggt gagagtgatg
     3001 gagaacgagg gcgtctggtt cctgcaatgc agaccgaaga tattatttca gttatcaatt
     3061 gggcagaaaa gcaggaatgt attgataatc agcgtattgg gttatgggga acatcgctgg
     3121 gaggggggca tgtcttcagt gcagctgcgc aggaccaacg cgtaaaatgt attgtcagcc
     3181 agctggcttt tgctgacggt gatgttctgg ttaccggaga gatgaatgaa tcggagagag
     3241 cgtcttttct atccacgctg aataaaatgg cagagaagaa gaaaaacacc gggaaagaga
     3301 tgtttgtggg ggttacacgg gtactaagtg atgatgaatc gaaggtattc ttcgagaaaa
     3361 tcaaagcccg gcatcccgaa atggatataa aaataccttt ccttacggtc atggaaacgt
     3421 tgcaatataa accggcagag agtgcagcca gcgtccagtg cccagttttg gttgttattg
     3481 ccggccagga tagcgttaat ccaccggaac agggaagagc attatatgat gctgttgctt
     3541 ctggtactaa ggaattatac gaggaagctg atgcatgcca ttatgatatt tatgagggcg
     3601 cgttttttga acgtgtagta gctgtgcaga cacaatggtt taaacaatat ctttaattgt
     3661 cagtatttat acgttaaagg tcgttgaaat taacttcctg ttacgatgag ccgtttcaac
     3721 ggcctgcagg gcatattttc acgaaggtca gagtaagcat ggcagagcaa aaacgaccgg
     3781 tactgacact gaagcggaaa acggaaggag agacgcctgt ccgcagccgg aaaaccatca
     3841 tcaatgtcac cacgccacca aaatggaagg tgaaaaagca gaagctggcc gagaaagcag
     3901 cccgggaagc ggagctggcg gcaaaaaaag cgcaggccag acaggcgctg tccatttatc
     3961 tgaacctgcc cacgcaggat gaggccgtga acaccctgaa accctggtgg ccgggattat
     4021 ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta ctggaagacg
     4081 tggcgcagcg gaatatcccg ctctcgcata aaaa
//
LOCUS       DBFIFS010000294         4106 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.295, whole genome shotgun sequence.
ACCESSION   DBFIFS010000294 DBFIFS010000000
VERSION     DBFIFS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.295"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            122..838
                     /gene="nanC"
                     /locus_tag="N7A07_004503"
     CDS             122..838
                     /gene="nanC"
                     /locus_tag="N7A07_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU2479026.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            858..1964
                     /gene="nanM"
                     /locus_tag="N7A07_004504"
     CDS             858..1964
                     /gene="nanM"
                     /locus_tag="N7A07_004504"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU2479027.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2029..3009
                     /locus_tag="N7A07_004505"
     CDS             2029..3009
                     /locus_tag="N7A07_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU2479028.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            3132..3827
                     /locus_tag="N7A07_004506"
                     /pseudo
     CDS             3132..3827
                     /locus_tag="N7A07_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1160 a          934 c          963 g         1049 t
ORIGIN      
        1 aatacagcta ccggttgcca aagacactat aagcctggca aaaaaatatt acacaacata
       61 aatgctaatt gtttatgcgg gctttgtatt gctttctgta tcctacaaat gagtgaaatt
      121 tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt caccattaat
      181 ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc acgcctatga
      241 gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca tggaaagtaa
      301 tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg atgttcaggt
      361 tgaagttaat tacgcgatta aacttgatga tcaatggacg gtgcgcccgg gaatgttaac
      421 gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct gggacgcgac
      481 aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc gacaacaaga
      541 cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg tcacttacca
      601 tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac agaacgatta
      661 tcgctatgca aaccataaga aatgggcgac ggaaaatgca tttgttctac aataccatat
      721 gacgcccgat attacgccat acatagaata tgactacctg gaccgtcagg gtgtttacaa
      781 cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta aactgtagta
      841 gacaggagac agtcacaatg aataaaacaa taacggcgct tgctatccta atggcttcat
      901 ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtacc atttaaaagt ggtaccggag
      961 caattgataa cgacactgtc tacattggtt taggtagcgc aggtacggca tggtacaagc
     1021 tggagacaca ggccaaagat aaaaaatgga cagcgttagc tgcattccct ggtggaccaa
     1081 gagatcaagc aacctcggca tttattgatg gcaatctgta tgtgtttggc ggcattggca
     1141 aaaacagcga gggcttgact caggtattta atgacgtaca caaatacaac cccaaaacca
     1201 atagctgggt taaattgata tcgcacgcgc cgatgggcat ggcgggccat gtgacttttg
     1261 tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc aatggctatt
     1321 ttgaagatct caacgaagct ggaaaagatt caaccgctgt agataaaatc aatgcacact
     1381 attttgacaa aaaagcagaa gattatttct ttaataagtt tctgttgtct tttgatccct
     1441 ctacacagca atggagttac gctggcgaat ctccctggta cgggacggct ggtgcggcgg
     1501 ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaaccc ggattgcgaa
     1561 cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat aggcttgctc
     1621 ccgtctcatc accagatggc gtcgctggcg gttttgcggg gataagcaat gattctctta
     1681 tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccaaaac ggtaagaact
     1741 atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg cataacggga
     1801 aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca ttgccctgga
     1861 ataatagtct attgattatt ggcggtgaaa ctgcaggcgg caaagcggtg acggattcag
     1921 ttttgatctc tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc agggccccgg
     1981 taaaggggcc ctgctatcaa tttgtcattt caattacgcg aaatttatat gaacgcaata
     2041 atatcgcctg attattacta tgttcttact gttgctggtc agtctaatgc catggcgtat
     2101 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc atcccagaat taaacaatta
     2161 gcgagatttg cgcatacgca tcccggaggc ccgtcatgtc actttaacga cattattcca
     2221 ctgactcact gcccacacga tgttcaggat atgcagagtt atcaccatcc tctggcaacg
     2281 aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg gaaattactg
     2341 cccttcattc ctgataatgc agggattctc atcgttccgt gttgccgtgg cggatcggct
     2401 tttaccgcag gcagcgaggg gacatattca gaacggcacg gagccagcca tgatgcttgt
     2461 cgttggggaa cggatactcc gctataccag gatttagtca gcagaacgcg agccgcactg
     2521 gtaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
     2581 atgaccagtg actacgcgtc acatcctcaa cactttaatc atatggttga agcctttcgt
     2641 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc gtggttttgc
     2701 ggcgatacca cctggtactg gaaagaaaat ttccctcatg cgtatgaagc tatttatggc
     2761 aattatcaaa ataatatttt agctaatatt attttcgtcg acttccagca acaaggtgca
     2821 agagggctga cgaacgcgcc tgatgaagat ccggacgatt taagcacggg atattacggt
     2881 tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca tttcagctca
     2941 gcagcccgtc gggggattat ttctgacagg tttgtagaag caattttgca gttttggcgc
     3001 gaaaggtgag cgttcattaa tttgatttat tcgcatccat cagcgctttt aactcctccg
     3061 gcgtgcggca ctataacgac ttccacaaca aggagccatt ttcttatacc actttcagtg
     3121 accatccgct tatgccacca ccctctacgc aactgtttgc agagcacctt cccaccgagt
     3181 ggatacagca cttcctgacg ctttctgctc atgcgaccgt tcgtcgtctt aacctgagcg
     3241 tggatggcga agcggggatg aacctgctgg cgcccgcagc gctgtcaccc aggcgcgtca
     3301 gcgcgtgggg gccgccccgg tggaatggct cttccgccag accacacaga catggggcgc
     3361 ggaacgttac ctgaaggatg actggcacgg cctgcaactt tttgccattg atggcgcaca
     3421 gttcaggaca cctgatgaac ccgagctgcg tgaatattat ggctctgcca acacatccac
     3481 tgagcggcag agcgcctacc ccgtcatgcg tctggtggcg ttaatgaatc tgggaatcac
     3541 attctactga atgccatgac cgcgcctacc ggcggagcga tacggcgtga ggcaacaaaa
     3601 gcggcgtcag agatcagctt taagttcgcg ttccagttta tcgtgacaga aatgatagtg
     3661 ctgggaaaca cggcgtcacc ggggactatc ccgaaacgac tggagcatct gcgggggctc
     3721 tggaagtggt gttcataaca aaacgccccc gaccatcaag ggcgagggcg gttaagatat
     3781 caaaaacccg ttatcctgtg aaacacagtg ccgccccgct taagtgaacg gcattgtgtt
     3841 tctgagggac cttaattttc cctgattctc tgacgttcct taccattgat atttccttca
     3901 ttcctcacca gagaaataaa aaatcactat actcatcaaa atatgagatt acctttattc
     3961 aggtgatcac cttactattt tttacggaaa ataaattatg aataacaaga aatcactgac
     4021 aaaccacctg taaaatgtca atcattttag ttcaattgcc tttgtcatga ttatcttttt
     4081 atcgtcctga cgatactatt aacaat
//
LOCUS       DBFIFS010000295         4023 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.296, whole genome shotgun sequence.
ACCESSION   DBFIFS010000295 DBFIFS010000000
VERSION     DBFIFS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.296"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1058)
                     /locus_tag="N7A07_004507"
     CDS             complement(<1..1058)
                     /locus_tag="N7A07_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU2479029.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLLRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRY"
     gene            complement(1068..2249)
                     /locus_tag="N7A07_004508"
     CDS             complement(1068..2249)
                     /locus_tag="N7A07_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU2479030.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(2317..3342)
                     /locus_tag="N7A07_004509"
     CDS             complement(2317..3342)
                     /locus_tag="N7A07_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2479031.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(3773..3994)
                     /locus_tag="N7A07_004510"
     CDS             complement(3773..3994)
                     /locus_tag="N7A07_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479032.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1128 a         1038 c          975 g          882 t
ORIGIN      
        1 ctatagcgcg acatgctgaa acagaagatc cagtagatca gcgcggcgaa aacataccct
       61 tccgtcgaca tacccaacca ggcgggatca acggttgctt gctgaacgct actgaaaaga
      121 tcgaacaacc cgataatgat caccaggctg gtatctttga agagtgcgat gatggtattt
      181 accagcccag gaattaccag cttcaacgcc tgtggcagaa taaccagccc ctgagttttc
      241 cagtaaccca acgccagcga ctctgccgct tcatattgcc ctttaggcag cgcctgtaat
      301 ccacctcgca cgacttccgc aacatatgct gactgaaaca ggatcacgcc aaccagcgcg
      361 cggagcagtt tgtcgatact ggtgccttct gccataaaca aaggcagcat gaccgaagac
      421 ataaacagaa cggtaatcag cggcacgccg cgccagaatt cgataaaaat gaccgataag
      481 atgcgcacaa tcggcatatg ggagcggcga cctaacgcca gtaagatccc ccacggtagc
      541 gcccccgcaa tcccaactga tgcaataatt aaagttagcg tcagcccgcc ccattgccgg
      601 gtttcaaccc gctcaagagc aaaaaaaccg ccatacatca gccaccagac aatcagtggg
      661 taaatcactg cccagaccgc aatatagcga ccgcgatgcg ggagtatttt ccagaacatt
      721 ggtgcgattg acacaaggcc aatcagtaat gccaagttaa ttcgccagcg ttggtcgtgt
      781 gggtaaagtc catacataaa ctgaccaaat cgttcgtgga tgaagaccca acaggcaccg
      841 gcttttgtgc agtcggcacg cgttgagcca acccagttag cctgcaaaaa tgcccagttc
      901 agcaacggtg ggatcagttc ccacatcaac catatgcagc caatagtcag caggctattg
      961 ctccagctgg agaacagatt ttttcgcacc cacaccatcg cgcggcttga gttatggctc
     1021 gccgggcgcg agggatgaga cagcaatact tttgtcatca tgactcctta gcgttccacg
     1081 atcgcgatgc ggcggttata gatattcatc agcagcgaga tagtcaggct gataatcaga
     1141 tagaccgaca tggtcatggc tatcgtctcg atggcttgcc ccgtctggtt cagcacggtg
     1201 ccagcaaaca gcgaaaccat atcgggatag ccaatagcgg cggcaagaga ggagtttttg
     1261 acgatgttga gatactggct ggttaacggt ggaataatca cccgcagtgc ctggggaata
     1321 atgacctggc gtagcgtaac cgggttgggt aatcccagtg accgagccgc ttcatgttga
     1381 ccataaggca ctgcctggat ccccgcgcgg ataatctcgg cgataaatgc agaggtataa
     1441 accgaaagtg ccagcgttaa ggctgccagt tcaggaatta aaaccatccc gccgcggaag
     1501 ttaaagcctt gtagggctgg gacatcccag tgtagtgctg cgccaaaaag ccattgcgcc
     1561 agtaaaggca aaccgatgat caacactgcg gcgatgggcc aggtgcggcg cagctgtccg
     1621 gtctttatct ggtatgtttt attaaagcgg aatagcccaa cagaaaggac tatagccata
     1681 acaacagcca ggataaacgc aataaatccg tctcccagct gcggtgacgg aatataaagc
     1741 ccacgattgc tcaaaaaggc cagatcaaac gcgctcacag cctggcgtgg tccgggcaaa
     1801 ttgcgcaaca cggcaaagta ccagaagaag atttgcagca gcgggggaat attacggaag
     1861 atctcaatat aaattgtgga aagctttcgt agcagccagt tatccgaaag tctcgccaga
     1921 ccgataaaga agcccaaaac agaagcgaac acaatacaca atgcagaaac cagtagcgta
     1981 ttgagtaagc cgacaataaa aacgcgtccg taggtgtcgc cctgctggta atcgatcaaa
     2041 tgctggacaa taccgaagcc agcgccgcga tccagaaagg caaaacctga agtaatgcca
     2101 cgattattga gattcgttac agtgttgtga aataaccaac caacaatgcc gacaacagca
     2161 acaacggcaa ggatctggaa taaccaggcg cgaaccgtag ggttggcaaa agagagtgag
     2221 cctttaacgg ttgagcggcg atgagacata gaaacctcgg aaagcataac gctgaagaga
     2281 gcgccgccgt agcgacgctc gttacctcac accgacttaa cgcacgggcg gtgcgtactg
     2341 aataccgccg ttattccaga gattattttg cccacgttta attttcagcg ggctttctga
     2401 acctacgtta cgctcgaaaa tttccgagta gttacccacc tgtttgatga tgttataggc
     2461 ccatttatta tccaacttca gatccttgcc gtaatcgcct tctttaccca gcagatgtgc
     2521 catatcaggc gttgctggat tagccgcttt ttcatcgacg ttctgggaat tgatgcccat
     2581 ctcttcagca tttagcatgg cgaaaagcgt ccagcgtaca atcgagaacc attcatcatc
     2641 gccacgacga actaccggcc caagcggttc tttagagata acctccggta agacaatcca
     2701 ttcagctggg ttgcttaatt tgatgcgcag ggcatacagt tgggattgat ccgaagccag
     2761 cgtatcgcag cgaccggatt ccagtgcctt cgctgattcg tcagaacgat cgaaagtcac
     2821 tggtgtgtac ttcatattgt ttgccttgaa atagtcggcg acgttgagtt cggtatcagt
     2881 acccgcctga atacagacgg tagcgccatc cagttctttc gcgcttttta gccccgcttt
     2941 atcgtgcgtc aggaagccaa tgccgtcgta ataagtgacg ccagtaaatg ccattcccat
     3001 cccggcatcg cgagatgaag tccaggtcgt attacgggag agcaaatcca cttcccctga
     3061 ctgtaaagcc gtgaagcgtt cttttgctgt gagtggggta tatttcactt tcgtgtcgtc
     3121 accaaataca gcagcggcaa caccacgaca aacatcaaca tcaatacctg aaaacttacc
     3181 gtctgcatcg gcataagaga acccaggtaa tccatcactg atcccgcatt gcacaaaacc
     3241 ttttttctga acggcatcaa gcgtcgcgcc agcatgcgcc tgatttgcaa cggcaagcag
     3301 cacgctggcg gcagccagtg tggctatcat catctttttc ataatgcttc ctgtgaggcg
     3361 aaaattatcg ttataggacg tttgggaaac gtcttttgcc tgtctgtggc catcctctta
     3421 taagcaaaag tagtgccagc ttttgcgcac gatttcgcgt cgcaataagc tgagtgtaga
     3481 caggaaataa taaaaacagc gccccgcaat ggtgcgaaaa tgaaacatgc gccatatttt
     3541 ggcgcaaaac atttacgcgt gttttactaa gcagaataat gattgttagc gtgagtatat
     3601 ttctggtggg aagtgcgaaa gttaaattga tgcgttattt cgaaagcagc gaaataacac
     3661 gatataacat gctaaatcag cagagaatta gccagcagaa tatagcaata ttttatctta
     3721 tgtaaagata ctgtatcgtt aaacagagcg cgaaactccg cgccctgttc agttatttcg
     3781 acagagcgat gatccccatc gtcaggccag caacagccgc tgccccgcca gcaatagccc
     3841 cagccgtttc ccagttatcc tgagtcgtgc ctttcataca ggtattggtg tgaaccagac
     3901 gaccctgatc gtcataaacc ggaacgcagg gcgaatcgtg agcacagccc gccagcaatg
     3961 cggtgagcaa tgccacagga attaatcttt tcatggtgtt acctcagtaa taaacgctaa
     4021 cgc
//
LOCUS       DBFIFS010000296         4017 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.297, whole genome shotgun sequence.
ACCESSION   DBFIFS010000296 DBFIFS010000000
VERSION     DBFIFS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.297"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..731)
                     /locus_tag="N7A07_004511"
     CDS             complement(<1..731)
                     /locus_tag="N7A07_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU2479033.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVG"
     gene            complement(822..898)
                     /locus_tag="N7A07_004512"
     tRNA            complement(822..898)
                     /locus_tag="N7A07_004512"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(862..864),aa:Arg,seq:tct)
     gene            complement(912..988)
                     /locus_tag="N7A07_004513"
     tRNA            complement(912..988)
                     /locus_tag="N7A07_004513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Arg,seq:tcg)
     gene            complement(996..1071)
                     /locus_tag="N7A07_004514"
     tRNA            complement(996..1071)
                     /locus_tag="N7A07_004514"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1513..1947)
                     /locus_tag="N7A07_004515"
     CDS             complement(1513..1947)
                     /locus_tag="N7A07_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2479034.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(1940..2134)
                     /locus_tag="N7A07_004516"
     CDS             complement(1940..2134)
                     /locus_tag="N7A07_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU2479035.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(2131..2736)
                     /locus_tag="N7A07_004517"
     CDS             complement(2131..2736)
                     /locus_tag="N7A07_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2479036.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(2736..3458)
                     /locus_tag="N7A07_004518"
     CDS             complement(2736..3458)
                     /locus_tag="N7A07_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU2479037.1"
                     /translation="MSELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(3451..3660)
                     /locus_tag="N7A07_004519"
     CDS             complement(3451..3660)
                     /locus_tag="N7A07_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU2479038.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCSDCCAELMSDPNSSMYEEEDDE"
     gene            complement(3620..>4017)
                     /locus_tag="N7A07_004520"
     CDS             complement(3620..>4017)
                     /locus_tag="N7A07_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479039.1"
                     /translation="LIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREATN
                     YWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYKKAWKEHRDRYQQDMEKLES
                     ENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT          952 a         1038 c          879 g         1148 t
ORIGIN      
        1 ctccccactc tgacaccatc ctctccccga tactccggaa gcacattgct gattcgcccc
       61 cagttcagag tgaggtccac gtctcccggc gtcatcgtat acacaggagc agtttcagac
      121 agtgcctgac gaaattctct ctgtatctgc ctgaagcgta aggcttctgc tgtgacagtg
      181 acaaaacgca gaactgctct ggatgcatct ctggtcattg tattaccact gaactccatt
      241 aacgccagat atgatgaaac cagtgagtga cgactgattt gcattccgga acgttccagc
      301 gctgcgacac gttgcagagt ggtataactg ctgtccgttg tcatggaaac cgttgtcaca
      361 ccgggcactg atatatgtgt aaaatctgaa aaacggtaga aagtatttgt tgccgtatta
      421 acgaacccgg ccacatataa attattttgc tcaataatca gacgaagatg gtcaaaacgc
      481 gcctgataga catcaagccc tcgtatatcc acagcaaaat aactgcccgg tggggtgtgg
      541 ttaataacag acaccgatgt ggtcccctga gatatatgtt caagaggggt cgatatctct
      601 gttcgtatac tatttaacga agagacataa ctttgttggg tcgaaaagtc tatcgtaaac
      661 tcccgggaat aggataccga agaaaaaccc agtaacaggc acagtaccca tttaaataat
      721 atacacttca tatacaggtg ttccttttgg ctgaagtaat cagcaccaga cccggcgcag
      781 atataaaaaa ggcccgcaaa agcgagcctg gtaaataaat atggcgcgtt gtactggatt
      841 cgaaccagtg accgattgct cagaaggcaa ttgctctgtc cggctgagct aacaacgcat
      901 aatgcagata atggaccgcc atcgaggact cgaaccccgc gcagccagct tcgaaggctg
      961 gcgctctatc ccgatgagct aatggcggta tgtgatggtg gcccttgctg gatttgaacc
     1021 agcgacctgg cgattatgag tcgctcgctc tcaccactga gctaaagggc cgggcgcagg
     1081 ataataacgg tacgtaacta attctgcaat atcatccgtt ctgactgact acattctgaa
     1141 cttccctgac cgtctgctca aaacgcccgc tctccagctc aacgccaatt gcacgacgcc
     1201 ctagcgccat tgctgctttg acgctacgga cataaaaaag ccagccactg ggggaggctg
     1261 gcaaactcgt agagcaaaat gctgttacgc aaacttcgtt acagggttat cctgcaatac
     1321 ttaaaatata caatatttag aaaactaata gtgctatatg cgatttttaa gattttgtta
     1381 ttaattacgg tcgcaccttc ctttctgtgt actttccgta tagctcacag gattcggggt
     1441 acaaaaaaac ccgcgcatcg gcgggttcga ctgcgtggca atgtaaccac tcttatcatg
     1501 atatgcagat ttttacgatc gtaaactatt ttttcgctga taaaatacag aggttctccc
     1561 tcccggcaat tcacgctcaa cataccgatc catctcaagc ctcactccca gcatcatcag
     1621 catgccttca acaatcccct ccgctttgtg aaggcgttta cctatacagg tgtcagagca
     1681 cccatgtttc cgtgccaacg ccatgaacgt ctcccccaac acgtaataat caaccagcaa
     1741 gtcatgcaga tcgcgattgt tccggtaaag gcgggctatg cacccgcata tcaccatcgc
     1801 atcatcgtca cagcactgtg gacgtgattt tactttttcg gggatcagtc ccttaaatcc
     1861 ggcagcaatg ggcgaccatg taacatcctc atagttattt gccgcccatg ccccccagcg
     1921 ctcaagaacc tgccggatat cacgcatcag tatctttacc ccatccgcgg tgaaccataa
     1981 gaacaccgtt gacgatagcg tgtcttttcc cttctttatc gccagtgtat tttctgaccg
     2041 tgttgcgact acagttcagt attctggcta cctcggtctg attttcatat gcctcaacga
     2101 gcatgtcagg aatggttttt actgtgaacg tcatgcggcc tcacttctgc tgtttcgcag
     2161 gtctttaagt ttctgctgat acttcgcctt gatcgccctg cattcttcga cagtccagcg
     2221 atggcggtta tggttcgatt cgatttcgtc tactgcttcc tgcccgatgc ggttaatcag
     2281 ttcgacgcga tacggaacga gatttccgct tttgtgctgg ttgcacacca cgcattgctt
     2341 gtgaatattg cgttcatcaa atcggagttg aggtgtcgca gcagttgtcc ggtaatgtcc
     2401 ggcatcccac tgagcagacg tgagcgttcc gcacgagata catggtaagt cgcggtctct
     2461 ttctctgatg aaggcgttta cggcttgttg ggcttgttta atccagtaac tgcggggctt
     2521 taaggcgagt tttcgaatct tcagtttatc tttctgtttc tgctcctctc gtcgtcgttt
     2581 cttctctgct gctttttccg ctttttcgcg ttctttactt cgtcgttcga gtgctaattg
     2641 agttccgtgt tccgggcagc accaccactg atttgagaat gccgggtgaa accattcctt
     2701 acagattttg catttccttc gcgctggttt agccattaag cagcctcccc tgttacttta
     2761 agcattccgt tatctagcag ctttcttgtc agccactgtt gaccacgccc ggtgattttt
     2821 gtggtgaacg atatctgtat tccgtgattt gtattgaccg ctgtttcttt cactgtgaaa
     2881 tagccgcgat ccatatattc ctgcattggc acattgcgcc gggcacctga agcaataagg
     2941 attttgtgat cgcgcatcca cgcaaacagt ttgtttggac caataccaac aacctttgca
     3001 aagtttccaa tcaaaattcc gctggcctcg ccaacgcgat cggcaaactc aactttaggt
     3061 gcggcaattg cgagctggtt ttccagttgc attttctgct cagcaagatc agcagcaagg
     3121 cgcaacgctt ctggtagcgt tttggggata ttaaccgcag cttcttcaag ctctcgccag
     3181 cggtcaacaa ggcgagcggt gaattccggc gacagctggg ctacaacgac aatactgtct
     3241 cgctttcctt gttcgccttc gaagacgtaa tgctcgtact gaacattgaa ccctaagtta
     3301 ttgattcttt cggaaacctc aatttgagga agccggataa caccattttt agccagcgtt
     3361 tcgatggtac gtttcacatt gtcatgacgc ttacccacca actcagcgat ttcaatgctt
     3421 gtcattttga tggcattgcc atttattaac tcactcatcg tcttcttcct cgtacattga
     3481 gctattcgga tcgctcatca gttctgcgca gcaatcggag cacacgtgaa cttccagcac
     3541 atgcagcttc tgaccgcagt tagcgcacgt taaagcccgc tcgacgcttt ctttctggta
     3601 ttgaagggat tgggatggac taagcatggc tttcaccatt aaaaagtcgc ttgtaagcat
     3661 caatgtctcg ttttgcttca ccgagctttc gtcttaattc catgttttct gattcaagct
     3721 tttccatgtc ttgctggtat cggtcgcggt gttctttcca tgctttttta tacgccttca
     3781 tgtatgtcat gttggccttt ctctttgcct gacgaactgc gtggtggttt ttcacaaacc
     3841 agtcagggtc gttaaatgct gctctggcgc aggtatacca ataatttgtt gcctccctgt
     3901 ttagccaata aatactgata aatggcaacc ggatagacac catttttcgt tgagactctt
     3961 tctcgccaaa catgtgccct tttttgatgc taaggccaaa tccaggttga attaaaa
//
LOCUS       DBFIFS010000297         4014 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.298, whole genome shotgun sequence.
ACCESSION   DBFIFS010000297 DBFIFS010000000
VERSION     DBFIFS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.298"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..3766)
                     /locus_tag="N7A07_004521"
     CDS             complement(<1..3766)
                     /locus_tag="N7A07_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HGU2479040.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLA"
     gene            complement(3823..>4014)
                     /locus_tag="N7A07_004522"
     CDS             complement(3823..>4014)
                     /locus_tag="N7A07_004522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479041.1"
                     /translation="FSPKEQGWGTFKLLCFSKLGSSPFDEKTITLSCFFESLFQLIFR
                     DFLLRQVIGFAAIIFADIP"
BASE COUNT          951 a         1052 c          895 g         1116 t
ORIGIN      
        1 acgctaaacc gtagaagcca ccgagaacag cgtctttacc gctattcgta accttatttg
       61 ccgatacgcc agcgacacca taactcagca tattgaggta gttataaata ttaccggtgg
      121 ttttaacacc cagagaataa tcacccacaa tatttccacg gttattaatg tcattcacgg
      181 cattgatcgc gatggtttgt cccaacatat tactgttgct gttgttggta acatacttct
      241 gcgtatcaat agtcagcgca gtacccgcca caatgtcttt aaagttctca acaccgttca
      301 gggcattaat ggtcagcgcg ttgtccgccg cgatggcatt acggttatac agcgtgcctt
      361 tcagaacatt gaaatgcaca tctcctttac cgctgatcca accatagtta gtgacactgt
      421 tatcaacgct cagatccagg tttttgtcag acgcaatcac gcccgtggcg gtattgtctt
      481 ccaggcgacc atcgctggcg ttgttcaacg acgccgcata aacgtcgaga ttaccggcgc
      541 tgtacgtggt ggcatagttg ttgtagaacg tccctgccgc acggatgatg gcatcagccc
      601 cactgctaag aagcgcgcgg gtattatcca gcgttcccct ggacagcaga ttaagcggac
      661 cattttcagc gatgatgctg ctgttattgt tgtagacgtt ctgagcagaa agcgtgacac
      721 cttctttacc tacgatgccg ccaacgttgt cggtaattcc aaacagcgcg ccgttattac
      781 gcaggaagtt attcccgtcg cggttttcga ggttgccgcc agcgttaata accagctttt
      841 tattcgccgc gatcagcgca gtgttgttgt aaatcgtcga tttagcatcg acagaaacat
      901 tctcaccggc aatcaaacca taggcgttgt ctacaatgtt cgccgtcaga gtcacgtcgc
      961 ccgtttcaga ggtcagcgaa cccttatagc tgtcgtagtt gcccgtcagt gccagcgtga
     1021 catcgccata accacgaatt tcaccgttat cgttgttcag attgttgcct ttaacagaca
     1081 acacgccgtt caacgcatgc agattaccgc ggttgttacc cacgttaccc ttcgaatcga
     1141 taatcagatc gcctgtcgct tcgatggtcg cgttattgtt cgccacgcta tttgccgaca
     1201 atgcgatatc cccataccct accatataga gatagttata caggttgcct ttggtcgtca
     1261 cgttcaggtc agcgtcactg acaatgttgc cacggttgtt gatatcgtta accgcgttaa
     1321 taacaatatt ctgccccacc atattgctgt tggagttgtt ggtcacatgg cgattggtgt
     1381 tcatcgtcag gtcgccgcca gcagaaatat ccttccagtt ttcaataccg ttcaacgcgg
     1441 caatatccag ccccttttca gccgcaatgg tattgcggtt atacaggttg ccgttattca
     1501 ccgtgacatc aacatcgcct ttaccgctga tccagccgta gttggtaagg ctattcacca
     1561 cttccagtga caggttttta tcagatgcta tgaacccggt cgcattgtta tcgatcatcg
     1621 taccgctgct gctgttttgc agcgtggtcg cgtcgatatc caggttgccc gcactccagg
     1681 tggtagcgta gttgttataa tacgttccgc caacctgaat agatgcatcc gccccgctgg
     1741 tgaccagagc acgcgtgttg tcgaacgtgt tctgcgcctg cagagtcaga ggaccatcct
     1801 cagcgataag acggctgttg ttgttataga tgttctgccc ggaaagctcg atgccttcct
     1861 tgccgaccat tccacccgtt tgctgaggca tgccgaagta cagaccataa gcattaccga
     1921 agttattgcc atcgcggttt tcgatactgc cgcgcgcatt tatcttcagt tttttgctgg
     1981 ccacgatcag cgctgtgttg ttattgacgc cagacatcga ctccatcgtg atgttctgcc
     2041 ccatcatgaa gccgccgtgg ttatccacgg agttggctaa cagggagata tcaccctctt
     2101 cagagctgat cacgccaata ttattggtca ggctgccgcc aacggcgact tcaataccct
     2161 gcttaccgct gatccccccg gtatcgttac gcagagagct tcccttgata atcactttgc
     2221 tgttggacag aattttgccc gcatagtcgt cgatcgtgct gtaacttgac agggagacgt
     2281 tgccattaga cgcaatctgt ccgccgttgt tattgatgtt gttcgcacca atttctacgc
     2341 cggtatctgc aataatgccc agagagtcag aactgccggt atcggcggtt ttggttgaac
     2401 cattatttac gctacctgcc gcgccaataa cgatatgccc ggtggacgaa cggatcagac
     2461 ctttgttgtt atccacatta ccgttactga taatggcgat atcgccggtg gtctggatat
     2521 caccgttgtt gttattcagc gcagcggatt caagacccac atagccaccg cgaatctgac
     2581 cattgctgtt gttgagcgtt ccggttttca gcgccacgag ccccgcttca atcccgacag
     2641 aactcccttt accagagtta atcagcgtgg cgttattggt atcaaccgcc agcattcctg
     2701 cagccgcaag cttgccgtta gtattgtcaa tcgtaccgct gttaacgtag atatcgccca
     2761 tcgtagagat gttacccgca cgggtattca cgatagtatt cttgttggta ttaagcgaga
     2821 tagtgcctac agatgtcacc gtaccggtgg tgttatccag agtaccattt gttgtcaggt
     2881 tgatcgtgct tgcagactga atctgggcgt tactgtttaa caggttacct ttggaatcga
     2941 tgctgacacc attaacaccc ccagcaataa cgccgaggtt gcgaaccccc acacctttct
     3001 cggtgctgac cagactgatt ttgttcgcat acattccgcc cagtttggca acatctacgc
     3061 tgtaaccgtt acgggacccc gtggcggata cgctaccggt cacctggcct gcggcattaa
     3121 catagttatt gccagcaaca acgttcagct catcggcaga cactttgccg ttaactacca
     3181 cgttacggga cagaatttct gtcgggctgg cgttatccag tttaccgagc gtaatggtac
     3241 cgccgttcac ggagtaaccg gccagcttgt catcctggat atccggcgtc ccggtggtta
     3301 aggtaagttt acctgtattg attgaaccgc caccgtttac agtaatacca ttcgggttgg
     3361 caataatcag atccgcttta tcccctgcaa cttccatcat cccattaatg gttgaaggat
     3421 ttttggaagt aacctcattc aggatcacct tcgccgaccc ggaggtcaga ttactgtttc
     3481 cctgaatatt tccggcaagt gaagtactgg attcattagc gctattattg aaaacgacac
     3541 catttttatc aacgtttagg ttatcccaga tattatgaga taaaccgcta ccgttaggtt
     3601 tgttgatgtc aacgactggc acaccattgg cgttatatac cgttccattt tttatgacta
     3661 atcccgccgc ttgagaagct acaggaaaag acaacatcat aagtatagca ataggggtta
     3721 atgctttccc caccgttgct tccttactta aatttatcat cgccactaac gttttcctta
     3781 ttgatgctat tcattttgga catgcagatc acttttgatg actcacggaa tatcagcaaa
     3841 aatgattgcg gcaaatccta tcacctggcg caataaaaaa tcacgaaaaa taagctgaaa
     3901 taaggactca aaaaaacaac taagagttat tgttttttca tcgaacggcg aagaacctaa
     3961 cttactaaaa cacaataatt taaaagtccc ccatccttgc tccttgggtg aaaa
//
LOCUS       DBFIFS010000298         3947 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.299, whole genome shotgun sequence.
ACCESSION   DBFIFS010000298 DBFIFS010000000
VERSION     DBFIFS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.299"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            123..488
                     /gene="mdtJ"
                     /locus_tag="N7A07_004523"
     CDS             123..488
                     /gene="mdtJ"
                     /locus_tag="N7A07_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU2479042.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            475..804
                     /gene="mdtI"
                     /locus_tag="N7A07_004524"
     CDS             475..804
                     /gene="mdtI"
                     /locus_tag="N7A07_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU2479043.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(843..1664)
                     /gene="ydgD"
                     /locus_tag="N7A07_004525"
     CDS             complement(843..1664)
                     /gene="ydgD"
                     /locus_tag="N7A07_004525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU2479044.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1764..1847)
                     /locus_tag="N7A07_004526"
     CDS             complement(1764..1847)
                     /locus_tag="N7A07_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479045.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(1940..2248)
                     /gene="asr"
                     /locus_tag="N7A07_004527"
     CDS             complement(1940..2248)
                     /gene="asr"
                     /locus_tag="N7A07_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU2479046.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2519..2623
                     /locus_tag="N7A07_004528"
     CDS             2519..2623
                     /locus_tag="N7A07_004528"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU2479047.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(2672..3925)
                     /gene="ynfM"
                     /locus_tag="N7A07_004529"
     CDS             complement(2672..3925)
                     /gene="ynfM"
                     /locus_tag="N7A07_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2479048.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
BASE COUNT         1009 a          946 c         1003 g          989 t
ORIGIN      
        1 gcgcaacgcg ctttagtcct ttcttctgcc taagcttgcc ctatacgcgg ggcttgacca
       61 ctttactttg gtttcgctga attaagcgaa aattaaaata attctcttgc aggagaagga
      121 caatgtatat ttattggatt ttattaggtc tggctattgc tacagaaatt accggtacgc
      181 tgtctatgaa atgggcgagc gtcagtgagg gaaatggcgg ctttatttta atgctggtga
      241 tgatttctct gtcgtatata tttctctctt tcgctgttaa aaaaatagcc ttaggtgtgg
      301 cttatgcgct gtgggaaggt atcggtattt tatttattac cttgtttagc gttttgttat
      361 tcgacgaaag tttatcgctg atgaaaattg ccgggttaac caccctggtc gccgggatag
      421 tgttgatcaa atcaggtacc cgtaaagcac gtaaacctga actggaggtg aaccatggcg
      481 cagtttgaat gggttcacgc cgcctggctg gcattggcaa tcgtgctgga aatcgttgct
      541 aacgtctttt tgaaattttc tgacggcttt cgtcgcaaaa tatttggctt gctctccctg
      601 gcggcggtgc tggctgcctt tagtgcgctt tctcaggccg ttaaagggat cgacttgtct
      661 gtcgcttatg cattgtgggg cgggtttggt attgccgcca cgttagccgc aggttggatc
      721 ttgtttggtc aacggttaaa tcgtaaaggc tggattggcc tggtcttgct gttggctgga
      781 atgatcatgg tgaaacttgc ctgatgaaga cgctgcccgc gctgtcgggc agcgtttgaa
      841 cattattttt gcgacagttg atccagcttg tcgcgaaaac cggtaacaga aatggcccgg
      901 ttatcggcgc gccagcgatc tttcgcggca ggagccgaac tttgcacccc aattaattgc
      961 cagccgtcat cggtatgcaa catcagaggc gaaccgctgt cacccggcaa ggtatcgcac
     1021 tgatgtgaca tcaccgacgt ttgcgcccag ccagtcactt cacagttttg atgactgtac
     1081 aacgtatcga gatgatcttc agggtatcct gcctgagtca ctttacgacc tgccgctttt
     1141 aatgcggcgg taagcgcggc tttatctccc tcaaataacg gcaacggcgt aatgccagaa
     1201 gggggattac gtagcacaat caatccgaag tcccacggcg cggctgcggg aggtacaatc
     1261 caaccatccc catctgcttt taaccgcttt cccagtgtcg gatcaacgcg gccttctatg
     1321 tcgtggatgt catagcgcca aagaccttta tttgacacaa aacgcagcgc cactgcttta
     1381 tcggctttac cctttggtgg cgtcaataaa cagtgtcctg ccgttaatgc cagattgggt
     1441 gcaatcagcg tcgccgtaca taaattgccg ctggccgttt ccagttgccc aaccgcatcc
     1501 cacggtgatt gggtcgtgtc attcactggc acacgatcat catgaccaaa aaacagggtg
     1561 ctgacctcat cgtttgccga tttggcaacg tctggtttat ctgcaaacac aaaagcagac
     1621 gtcaaactaa ttgcacccaa cactacagca atggttgtac gcatatcaca ctctggtggg
     1681 taattatgat tattaaaagc aaaccctcat aaatactata gacgggacgg gatgaaagtg
     1741 ggagtaaaat cagataacta cgatcaggaa agataaaaac gggcggcaat cagtgcgcat
     1801 aaaataagga tgataagaat gaactcaaag cgataacggc ccaccatact accctccgct
     1861 aaaggcggtg ctaaacgtta cccgcttagc accgtaattt tcaggcgcga gggggcgcgc
     1921 cagcattact gttgaaaact tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat
     1981 ggctgtgttt cttggcgtgt ttcttcgctg cctgcgcttt ttgttctggg gctttctgtt
     2041 cagctttcgt atttttatga tgctttttag ccgcctgcgc tttctgggca ggtgctgctt
     2101 tatgctgttt tttatgatgt gtagttttcg ccggcgctgc tttggtggtc gtcgcagtcg
     2161 gagcaggtgt ggtcgcagtc tctgcagcaa aggcggcaga agacagaccc atagcagcgg
     2221 caacaaccag agctaatact tttttcattg tcataccctc aatttgtttt ttcatttaac
     2281 cccactgcgg ggccgttgaa agaactatat ccctgggaat ggcagacttc cgtgagtggt
     2341 tggtttcagc gtgtaacgat atgtacaaac gctgaataaa ttacagcgtt gataataacg
     2401 tattgtgact taaggaaaat ttagctacca ataatagtag tcttgatcgg gttaactatt
     2461 ttactactta caaagcggta gaataactgc gcatcagtaa taaatgacac acagcaaaat
     2521 gaatccgttt atttgggtac ttataatcct gatgacgcta gacgcgctgc gggaattggc
     2581 tggcgcttcg tctattttag gatggctatt aacgttggtt tgagcaggca ataagtccgg
     2641 acgggtattt accgcagtcc ggacttattt ttcaggcgtg cagacgacga tgcaaacgcg
     2701 tcccgaccag cagagcaatg accagcatca gagcaataaa tgcgccgacg ccgttccagc
     2761 catagttatg ccagaaaaca ccacccagcg tcccggcaat actcgacccc agatagtaac
     2821 tgaacagata cagcgaggag gcctggcctt tagcgcgttt tgcgcggggg ccgatccagc
     2881 tgctggcaac tgagtgggct gcgaagaatc ctgctgagaa gagtaacatt ccggcaaaga
     2941 tcagccacag cgagctgaat aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa
     3001 acaacatcac tggaccacgc ccatagcggg tggtcatggt tccggctttg ggtgagctcc
     3061 atgtaccggt caaataagcc agcgataata agccaaccac ggcctgactg acatgccagg
     3121 gggagagcat caaccgatag ccgatgtaat taaacagcgt gacgaacgac cccatcagca
     3181 aaaagccttc tgcgaacaat aacggtaatc cccggtcacg ccagtgcaga cgaaagttga
     3241 taaacaacga cttaggtcgc agcgaagtcg ggcgaaaatg gcgtgattcc gggaggattt
     3301 tccagaacat caacgccgag gctagcgcga aacaaccgat agccgccaga gcaattcgcc
     3361 agttgaaaaa gtccgtgaag acaccactaa ttaagcgtcc gctcatgccg ccaattgagt
     3421 tgccgctgat atacaacccc atcgaaaagg ccacgaaact ggggtggatt tcctcgctaa
     3481 gataagtcat gccaacagct gccacgccac ttaacgaaag cccaatcaag gcgcgcataa
     3541 tcaaaatgcc gtgccagctg gtcatcattg tcgaaagtaa cgtacaaatg gaggccaata
     3601 gtagcgccgt taccatcact ggtttgcgac caatggcatc ggataggggg ccagtaaaca
     3661 gcaaaccaat agccaacatc gccgtggaaa tggacagtga aatactactg ttcgcgggcg
     3721 ttaagccaaa ctcctgcgaa agcaccggaa ggataggttg cacacaatag agaagtgcaa
     3781 atgttgccag tccggcagag aacagcgcca gggtgacgcg cataaattgc ggcgtaccgc
     3841 gtttaataaa ttgatttggc tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc
     3901 catcaacagt tgtagtacgg ctcacttgaa atccttgcta aatatgc
//
LOCUS       DBFIFS010000299         3936 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.300, whole genome shotgun sequence.
ACCESSION   DBFIFS010000299 DBFIFS010000000
VERSION     DBFIFS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.300"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(83..751)
                     /gene="artM"
                     /locus_tag="N7A07_004530"
     CDS             complement(83..751)
                     /gene="artM"
                     /locus_tag="N7A07_004530"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU2479049.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(751..1467)
                     /gene="artQ"
                     /locus_tag="N7A07_004531"
     CDS             complement(751..1467)
                     /gene="artQ"
                     /locus_tag="N7A07_004531"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU2479050.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1474..2205)
                     /gene="artI"
                     /locus_tag="N7A07_004532"
     CDS             complement(1474..2205)
                     /gene="artI"
                     /locus_tag="N7A07_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU2479051.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2223..2951)
                     /gene="artP"
                     /locus_tag="N7A07_004533"
     CDS             complement(2223..2951)
                     /gene="artP"
                     /locus_tag="N7A07_004533"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU2479052.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3169..3684)
                     /locus_tag="N7A07_004534"
     CDS             complement(3169..3684)
                     /locus_tag="N7A07_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2479053.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          997 a         1027 c          983 g          929 t
ORIGIN      
        1 acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc tacaaaaccc
       61 ccggcgaatg tacgcagcca cattaattcc ttcgctcaaa cgccagcgct ttgcgctcga
      121 tcagacgcat catcagcgtc agcaggccgt taacgaccag ataaataatc cctgccgcgc
      181 cgaacaccat gacatcgtag gtgcgtccgt acaacaactg gctgtatccc atcacttcca
      241 tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac cagcaccact tcgttggaat
      301 aagaagagag cgagcgttta aaggcatacg gcagcaggat cgccagcgta tcttttttgc
      361 tcattcccag ggcgctacag gactgccact gaccttccgg gatcgcacga attgcaccgt
      421 aaaacagctg cgtggtatac gccgcactgt tcagcgacaa cgcaatcagc gcacataacc
      481 acggttctga caacaaatgc cacagtgccg gatactcctg caacgtcggg aactgacccg
      541 gcccgtaata aatcaggaag atctgcacca gcagcggtgt accggtaaac agcgtgatat
      601 aaccccgcac cagccacacc agcaccggcg ttttcagcgt caggatgatg gtaaaaatca
      661 acgccagaat cagtgccaca atcagcgagg caacggttag cgtcaggctg gtgtgcagcc
      721 ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa aacgtgtcgc
      781 gcgcaggtca atgcgtttga gaatgtactg actgagcagg gtgatcacca ggtaaatcgc
      841 cgccgccaca atgtaccagg taaatggttc ctgggtacga gtagcgatgc ttttggtttg
      901 cagcattaaa tcattcacac taatcaaact gaccagcgcg gtatctttca gcagcaccag
      961 ccactggtta ccgaggccag gcagcgcatg acgccacatc tgcggcatca ccagacggaa
     1021 aaagatagcc gatttcgaca gccccagcgc ctggccggat tcccactgac ccatcggcac
     1081 cgctttcaac gcgccgcgca gcgtttgcga ggcataggcg gcatacagca gtgacagagc
     1141 gatgacacca caaaggaacg ggctaacgtc gaagttttca atctgcatct gcaccgggat
     1201 ctctacaatc ccaagattga tagtgaagcc atccgaaagt gtcagcagca gctgcgagga
     1261 gccaaaatag ataaacagca ccaccagaat ttctggcagg ccacgcagaa tggttaccag
     1321 cgctgaacct gcccacgcga caggacgcca tttcgcagac tcccataccg caaagaacat
     1381 cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg ccgacggtca tcccggcggc
     1441 gcttgctaaa ggaaaaaatt cattcatcag gaattacttc tggaaccatt tgttgtagat
     1501 ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca gtgttgagtt tctgctgcag
     1561 ctcagtgttg ccctgacgta ccgcgatgcc gaggccagta ccgaagtaat ctttatcggt
     1621 cactttatcg cccactgccg ccagtttcgg gttatctttc agccattcag ttaccactgc
     1681 ggtgtcacca aatacgctgt cgatacgacc gttttgcaga tccagttttg cgttctggta
     1741 gctgtcatac ggcacggtgg tgatttccgg gtgcttatcc ataatgaatt tctggtgagt
     1801 cgtaccattc tgtacgccga cttttttgcc tttcagctga tcaacgctgg tgtatttacc
     1861 ctgctgaccc acaaacaggg cagagttgtc atagtacggc gtggtaaaca gtacctgctt
     1921 ttcacgttcc gcggtgatat ccatcccggc catcaccgct tccacgcgac ggaatttcag
     1981 gcttgggatc aggctgtcaa atgcctggtt agagaaagta caggtcgcat caatctcttt
     2041 acacagcgcc tgtgccaggt cgacgtcaaa accaacgatc tggttgtttg catcaatcga
     2101 ttcaaacgga ggataggagg cttcggtagc aaaacgaatg gtttcggcag ctgtggcgga
     2161 aagactaaaa cctgcaatta acgcggcaat cagaactttt ttcattgttg ttatcccgaa
     2221 tcttagtgag agagatagtt tttaaatgct tcggtttgcg gctcggtaaa gcagctcgcg
     2281 tcgccttgtt ctacgatatg accgttttcc atatacacca ctcggctggc ggttttacgc
     2341 gccacttcaa cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc cagctcacga
     2401 atgatgctga cgatttgtgc cgtaatttcc gggtccagtg cggcggtcgg ttcatcaaac
     2461 agcagtacct gcggttccat cattaacgca cgggcaatag caacacgctg ctgctgacca
     2521 ccagaaagat gcagcgggta acgatcgcta taaggtttga gacgcagacg ttccagcagt
     2581 ttttctgcac gggccagcgc ctgatcttta ctcaacccca gtacacggca gggcgcttca
     2641 atcaggtttt gctgcacggt cagatgcggc cacaggttgt attgctgaaa caccatgcca
     2701 acgttacgac gcaaatcgcg aatcgctttg tctgagggtg ttttggtgaa atcgaaatgg
     2761 ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa gcagattgag tacacgcagc
     2821 agcgagcttt tacccgcgcc gctggggcca agtaacacca gcgtttcgcc ctgtgggcaa
     2881 tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga agcaattaat gccgtttaat
     2941 tgaatactca ttgacactcg tatactggca gtctgatagc tattgaggtc gaagatagta
     3001 cctttgacag aataattatg caatatttct gctttaaaag ttaaaagcaa agcgcattat
     3061 tcaataaaca tagcacaaaa taacgggggc ggtggtcggc gagcataaat gtcggcattc
     3121 ctcacgaaat gccggacaat ttacggggtt tattggttga tcaaggcgtt agcgattctc
     3181 gatggactga cggagcgtac ccgccgtggc atgaacgcta ccgcctaagt agcgcacatc
     3241 gtcgatcacc caacactggc cttcctgaat cattaacact tcatcctgcc agccctggtc
     3301 accctgcttg agatccacgc gcaatggaat gttacgggca tcacgattag ggatagtcga
     3361 tgcactggca acgtgggcgc tgtctggcaa ggtggttcga ctggagaacg gatcactgct
     3421 taacaattca cgatgactat tatcgcggct ggcatcgcta agtagggttg ccagtttgtc
     3481 actcaaatag gggcgcaggg cggtaatgtc attgctgcga tgttgaatgt ggtagtcata
     3541 gaattgttgg gccacgctgt caggcccccc ctggacacag gaaccggtgc ggggaccatt
     3601 atctttataa gctggcgtga cggtggtaca ggcactcagc agcagtgcac atggaataag
     3661 tagagatagt gaacggtagc gcataatcat ttccttatta gcttgctgtc tgcgtcacac
     3721 tacgaccaaa cgctatcaga ttatttaata cattgttttt aatacaattc tgaaacaatc
     3781 tgaacacctg attgggtttg agtccattgc tggcgaccca ctcaaggttg ctcgtagctg
     3841 acttacttaa attacccaaa caatagacag tgtaaaccat ttgtgaggtt gagcgtgtta
     3901 tatggctgaa atgatcattt aaggtaaata tgatag
//
LOCUS       DBFIFS010000300         3923 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.301, whole genome shotgun sequence.
ACCESSION   DBFIFS010000300 DBFIFS010000000
VERSION     DBFIFS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.301"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..185
                     /locus_tag="N7A07_004535"
     CDS             <1..185
                     /locus_tag="N7A07_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2479054.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="N7A07_004536"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="N7A07_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2479055.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="N7A07_004537"
                     /pseudo
     CDS             563..2148
                     /locus_tag="N7A07_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="N7A07_004538"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="N7A07_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="N7A07_004539"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="N7A07_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3923)
                     /gene="pitB"
                     /locus_tag="N7A07_004540"
                     /pseudo
     CDS             complement(2735..3923)
                     /gene="pitB"
                     /locus_tag="N7A07_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
BASE COUNT         1021 a         1024 c         1057 g          821 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgataaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aat
//
LOCUS       DBFIFS010000301         3879 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.302, whole genome shotgun sequence.
ACCESSION   DBFIFS010000301 DBFIFS010000000
VERSION     DBFIFS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.302"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..827)
                     /locus_tag="N7A07_004541"
     CDS             complement(<1..827)
                     /locus_tag="N7A07_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2479056.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLG"
     gene            complement(883..1215)
                     /locus_tag="N7A07_004542"
     CDS             complement(883..1215)
                     /locus_tag="N7A07_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2479057.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1225..2544)
                     /locus_tag="N7A07_004543"
     CDS             complement(1225..2544)
                     /locus_tag="N7A07_004543"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU2479058.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(2525..>3879)
                     /locus_tag="N7A07_004544"
     CDS             complement(2525..>3879)
                     /locus_tag="N7A07_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2479059.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDG
                     QTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQ
                     EQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLL
                     RYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLE
                     EAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTY
                     EKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA
                     "
BASE COUNT          768 a         1208 c         1061 g          842 t
ORIGIN      
        1 ttccccagca ccatcgtgtt gtccggcagg aagttctttt tgacgccgtt ttccacgtac
       61 tgtccggaat acacgacgat ggccacatcg ccatacattc ccttataaga caccgctttg
      121 cccaggtctt ttaccgctgt ctccagttcg gaatgagagc cgcgacgggt atccagcttc
      181 tccctgacgg ctttgaagga acggaacagc gcccagcctt tcgggtcaaa cacgatgata
      241 ttcaccacgc cgctggcgtt cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg
      301 gacttgtcac gcttgctcca ctccgtgccg ccggactgcg tgatgttgtt cgccgcactg
      361 cggcccatat ccacctcaac cggatcgaag gcttcaccgg tcatggtgta tttgcccttg
      421 agcacggcag aaactgcctg catctcttcg acctgagcaa tggccagctc ttcgtcacgc
      481 atgttctgca tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct
      541 tcatccggca ggcgacgcag ggtcatctgc ggattcactt catgcttcgg cttgacatat
      601 cccggcgtaa attcagaggt ggagccgcca cgggaacgga taacctcacc agaaacaatc
      661 ggcgaaacgt acagcgccat gtttaccagt cccggaattt gtgagagata gactttctcc
      721 gtggtgaagg gatagctctc acggaaaaag agacgcagaa acagcggatc aaacttaaat
      781 ttctgctcat ttgccgccag cagttgggcg gttgtgtaca tcgacataaa aaaatcccgt
      841 aaaaaaagcc gcacaggcgg cctttagtga tgaagggtca ggttaaacga tgctgattgc
      901 cgttccggca aacgcggtcc gttttttcgt ctcgtcgctg gcagcctccg gccagagcac
      961 atcctcataa cggaacgtgc cggacttgta gaacgtcagc gtggtgctgg tctggtcagc
     1021 agcaaccgca agaatgccaa cggcagcacc gtcggtggtg ccatcccacg caaccagctt
     1081 acggctggag gtgtccagca tcagcggggt cattgcaggc gctttcgcac tcaatccgcc
     1141 gggcgcggtt gcggtatgag ccgggtcact gttgcccagc ggctggtaat gggtaaaggt
     1201 ttctttgctc gtcataaaca tcccttacac tggtgtgttc agcaaatcgt taacggcatc
     1261 agatgccggg ttacctgcag ccagcggtgc cggtgccccc tgcatcagac gatccagcgc
     1321 agtatcactg cgcgcctgtg cactctgtgg tgctgcggcc agaatgcggc gggccgtttc
     1381 cacggtcata ccgggggttt ctgccagcac gcgggcctgt tcttcgcgtc cgtgagcctc
     1441 ctcacagttg aggatcccca taatgcggct gttttctgcc gcaaccgcag cggtgatctg
     1501 cgcgttcacg tccggctgcg ccgcgctggc gttttcgccc tccgtcgctg gcaccacgcc
     1561 agtaacgtca gcctgcgaag cagtggctga aacagttgtt gattgagtct ctttggtcat
     1621 tcgccctcct gagagacggg atttacgtgc atccagtgca tcacgcataa cggtgatcgc
     1681 atcggtgctg ttgacaagtt catcagccag tccggcatca atggcctcct gaccgctgta
     1741 cactgcagcc tcggtatcca gcacagcctg cacggacagg ccggtatatg ccgacacctt
     1801 ctgcgcaaac atccggcggg ttgcatccat ccgggactgc agtgtttccc ggacatcacc
     1861 cggtagatgg ctgtaggggt tgccatccac cttatggctg ccgctgtaaa tcagcgtgat
     1921 ttccacgccc tgtttctcca gggcagcacc gtaattactg tgagccatca tgacgccgat
     1981 ggagcctgtc cgggcggtct gcgtgaccag acgccgggag gcggcgctgg caagcagctg
     2041 acctgcactg cagttcatgt cgttggccag cgcccatacc ggttttatgt ctcgcacacg
     2101 ggcgatgatg tcagcacagt caaatgctcc cgccaccatc ccgcccggtg tgtccatatc
     2161 gagcagaatg ccgtccacca tcggatcgct ggcagcctgt tgcagacggg cgataatgcc
     2221 gttgtaaccg gtcattcccg aatacggctg cagcgcccgc gtccggctga ccagcgtacc
     2281 ggacaccggc agcacggcga tgccgttcat gacctgataa ctccgggcct gtcgtggtcc
     2341 gtcatcatca ccggataacg ccagcgccgc gggtgcctct ccggcagtca ggctgtcgcc
     2401 ggatactgca tccgtcaggc ggctgatccc aagctggcct gcaagcgcac aaaagaaaac
     2461 ccgcgcatag gcgggttcaa gcatcagcgg ctcattaaaa gccatgctgg caatatgcgg
     2521 gagattacgc agctctgctg tcactcttct cctcctctgt tgattgtcgc agcccggatt
     2581 caaatgccgc agccgcccag gcgggcggtt taagaccagc tgcgcggcgc tccatcgttt
     2641 cacggacctg ctgggcaaaa atttcctgat agtcgtcacc gcgtttcgcg cactctttct
     2701 cgtaggtgct cagtccggct tctatcagca tcaccgcttc ctgaacttct ttcagaccat
     2761 cgatggccat acgaccggag cctatccagt cgcagttccc ccaggcactg cgggcttcct
     2821 gaaagctgaa gcgcgctttt gaaggtaacg tcaccacgcg gcgaacgatg gcctcttcca
     2881 gccagcacag aaacatctgg ctcgcctgac gggatgcgac gaattttcgc cgccccataa
     2941 agtacgccca cgactcgttc gcactggccc gtgccgtgga gtagctcatc tgggcgtaat
     3001 tccgggaaag ctgctcatac gagacaccca gcccggcagc gatataccgc agcagtgact
     3061 gctcaaacac ggagtagccg ttatccgtgt cctgagccgt ctgcaggttc agtgagtccc
     3121 ccggcatcag gtgcggcact tttgcgcctc ccagacggac cggtgctgcg gcgtaatacg
     3181 cggcaatttc accaatccag ccggtcagcc tttcccgctg ctcctgactg ttcgcgccca
     3241 gaataaaatc catcgctgac tgcgtatcca gctcactctc aatggtggcg gcatacatcg
     3301 ccttcacaat ggcgctctgc agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct
     3361 ccatcacgct gtaaaacaca tttgcaccgc gggtctgccc gtcctccacg ggttcaaaga
     3421 cgtgaatgaa cgaagcacga ccgccgggta actcgcgggg tatccatgtc catttctgcg
     3481 gcatccagcc aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat
     3541 taatctgcac accggcacgg cagttccggc tgtcgctggt attgttcggg ttgctgatgc
     3601 gcttcgggct gaccatccgg aactgtgtcc ggaacagtcg cgagggacgg gtatcccagg
     3661 tggcctgaac gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca
     3721 tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcaaca gtcatcttcg gcaaactctt
     3781 tccatgccgc ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcccagat
     3841 agcgccagct tgggcgatga ctgagccgga aaaaagacc
//
LOCUS       DBFIFS010000302         3868 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.303, whole genome shotgun sequence.
ACCESSION   DBFIFS010000302 DBFIFS010000000
VERSION     DBFIFS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.303"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            288..2438
                     /gene="btsT"
                     /locus_tag="N7A07_004545"
     CDS             288..2438
                     /gene="btsT"
                     /locus_tag="N7A07_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU2479060.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2488..2691
                     /gene="yjiX"
                     /locus_tag="N7A07_004546"
     CDS             2488..2691
                     /gene="yjiX"
                     /locus_tag="N7A07_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU2479061.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2702..3658
                     /gene="yjiA"
                     /locus_tag="N7A07_004547"
     CDS             2702..3658
                     /gene="yjiA"
                     /locus_tag="N7A07_004547"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU2479062.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3727..>3868)
                     /locus_tag="N7A07_004548"
     CDS             complement(3727..>3868)
                     /locus_tag="N7A07_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2479063.1"
                     /translation="SIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLW
                     LV"
BASE COUNT          859 a         1038 c         1015 g          956 t
ORIGIN      
        1 atagtatgaa tataattttt aaatgagtta cacaaccgag ggctaaaaat cgcttcggtt
       61 gtgatcatca tcacataaat aaccttcata aaccttgctc aaaaccgctc aaccgcaaaa
      121 ctgaccgctt acgtccctaa aataaccact cagttattta ccttacttta cgcgcgcgta
      181 actctggcag catcattgca ggatagcggt caatttacct cctcaaacgc aacgcaaacc
      241 tagaacggct tcggccaact attaatcaat acatgccagg ttttactatg gatacgaaaa
      301 aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
      361 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
      421 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
      481 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
      541 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctag
      601 tgggaccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
      661 gggtagtgct ggccggtgcg gttcaggact ttatggtgct gtttatctcc tctcgccgta
      721 acggcgcatc tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg
      781 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgtcctggcg ctgattgtgg
      841 ttaaagccct ggcagaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccaattg
      901 cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg
      961 ttattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
     1021 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
     1081 atgcgtttgt ttccgcgctg ctgccggtgt ggctgatcct cgcaccacgc gactatctgg
     1141 caaccttcct gaagatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
     1201 cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag
     1261 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
     1321 cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca
     1381 tcggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgctgcat
     1441 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcccgcgggc cttggcatca
     1501 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga
     1561 aagacgttac cgcacacgcg gcagcgaccg tcagctcttg gggcttcgtg atttcgccag
     1621 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
     1681 gtgcgccgac gctggcggta ggtatcgctc atgtgttcca caaagtgctg ccgatggctg
     1741 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
     1801 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
     1861 cgttcctgaa aaaaaccgac tctctggttg ctggtatcat cggtactgcg ggctgtgtgg
     1921 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
     1981 tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca
     2041 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
     2101 tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc
     2161 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
     2221 accttacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
     2281 caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
     2341 cctggcttgc ggtacgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
     2401 tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc
     2461 ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat
     2521 ctcggccagg cggcgaagat gttgattggc attccagact acgacaacta cgtcgagcat
     2581 atgaagacca accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc
     2641 cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag
     2701 catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca aaaccaccct
     2761 gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt
     2821 tggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga tcaaaacgct
     2881 gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaagacgcac tactcgacct
     2941 gctggataat ctcgataagg gcaatattca gttcgatcgt ctggtcattg aatgcaccgg
     3001 catggccgat cccggcccga ttattcagac ctttttctcc catgagattt tatgccagcg
     3061 ttacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa
     3121 ccagttcacc atcgcccagt cccaggttgg ctacgccgac cgtattctgc tgaccaaaac
     3181 cgacgtcgca ggcgaagcag aaaaactgcg cgaacgcctg gcgcgcatca acgcccgtgc
     3241 accggtctac accgttaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg
     3301 ttttatgctg gaagaaaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa
     3361 acaaaacgat atttcgtcga ttgtggtaga actggattac ccggtagata tcagcgaagt
     3421 ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg
     3481 gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta
     3541 cagcgccgac tgggaccgcc cgtggggcga tgaaaaaccg catagcacaa tggtttttat
     3601 cgggattcag ttgccggagg aagagattcg ggcagcgttt gcggggttga ggaagtaagt
     3661 tcgggaaaga tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta
     3721 gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt
     3781 cagttcagcc aatccccatt ctgaagaatt acaaacatct tttgttaaat caaaagagct
     3841 tttactatta agtttctcat aaatagat
//
LOCUS       DBFIFS010000303         3809 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.304, whole genome shotgun sequence.
ACCESSION   DBFIFS010000303 DBFIFS010000000
VERSION     DBFIFS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.304"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..219
                     /locus_tag="N7A07_004549"
     CDS             <1..219
                     /locus_tag="N7A07_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2479064.1"
                     /translation="DYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSV
                     NVGYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            355..1146
                     /locus_tag="N7A07_004550"
                     /pseudo
     CDS             355..1146
                     /locus_tag="N7A07_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1151..2521)
                     /locus_tag="N7A07_004551"
                     /pseudo
     CDS             complement(1151..2521)
                     /locus_tag="N7A07_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2541..2888)
                     /gene="tnpB"
                     /locus_tag="N7A07_004552"
     CDS             complement(2541..2888)
                     /gene="tnpB"
                     /locus_tag="N7A07_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2479065.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(2885..3559)
                     /gene="tnpA"
                     /locus_tag="N7A07_004553"
     CDS             complement(2885..3559)
                     /gene="tnpA"
                     /locus_tag="N7A07_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU2479066.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          842 a          940 c         1033 g          994 t
ORIGIN      
        1 gattatgagt ggagaaacag tgtgggtgct gatgagtctg atggatattt aagtgagtct
       61 gtatcaaaaa aatccactga ttttgcttat gcagctggtg ttattattaa tccgtggggc
      121 aatatgtctg taaatgttgg gtatgaggga actaaagcag atatatatgg aaaacattcg
      181 gtcaatggat ttactgttgg agttggttat cgtttttaat cattagacgg gactggataa
      241 ttgcacttgc gtagtgaagt ttactaaatg tttgtatttt aatgaagtat tcgacatatt
      301 cccttcagac ggaatatgtc gacggttatg gttttactgg agcatgagta tgttatggcg
      361 aaactgataa atatatcagc gtccactgag gagagaatga cgccagggga gaggagggtg
      421 gcttcacgac tggagagctt tctgaatgat gattgctttg tctggtacga tatcccggtg
      481 ggacggaaaa accgacatcc ggattttgtc attattgacc cggataacgg gctggtattt
      541 ctggaggtca aggactggac tgtatccaca ttacgtaaag caaatcagga gcaggttaca
      601 ctggaaaccg acggtcttct gaaatcagaa ataaatccgc tggtacaggt tcgtcgttat
      661 gcctgtgata cggttaatgc cttacctgct gacccgtgct tacggcagaa tgacggtcag
      721 tataaggggc ggctgaacct ggcctgggcg tatggtgtgg tgtttacccg cattacccgt
      781 cagcaactga aggcactgac agggaataat gagaatgcgg tggaaaagat ttttccttca
      841 gcacagacaa tctgtcagga tgaaatgacg cagtctgttt tacctgaggt attccggcaa
      901 aaaattgccg ggatgtttac cacggggttc agaacccggg taacaccccg gatgagggat
      961 attctgcgcg cccatctgtt tcctgaagtg accgtgaaac agaactcaca aatcaaaatt
     1021 atggatattc agcaggagat tctggccaga aatattggtg acgggcatcg tgtcattcat
     1081 ggtgtggccg gttccggaaa aaccatgatt cttttgttcc gttgccttta cctggcagaa
     1141 accacgtccg gtagttacgc cgccccagtg caactacccg gagggcgttt tcacacaggt
     1201 tattgtcgat ctccacccag ccattgcggc agtattcgtt cagagcgtcc cattgcttca
     1261 gcagataggt gaacgctttc gctgtatccg agtggcgcga cagcgtgttc atctgcccct
     1321 agagccactc atacaacgac tgcattagcg gtaccgttct ggcttttctg accgccagtc
     1381 gctcttctgc cggactgccg cggatctcag cctcgatagc gtacagttcc ccgatacgac
     1441 ggagcgcttc tcccgttact gtcgttggat ggcggacatg tacatcgtgg atcttgcgtc
     1501 gggcatgtgc catacaagcc gcttcggttg cctgaccgct ttcgtaaaga gcattgtaac
     1561 ccgcatatgc atcggcctgc aggatacctc tgtagtccgc cagatgttgc tgtgggtgga
     1621 tgcctttgcg gtcgggagag tatgagaacc acaccgctgc cggcatggtc gaaccggcgt
     1681 tgcgatcgtc acgaacatag acccacagcc gtccggtacg ggttttaccc tgacccggct
     1741 ccagtacatt taccggcgtg tcatcggcat gcactttacc cggcatcagc acatagtgct
     1801 tcagttcatc atacagcggg cgaagctgct ctcccatgat gtcaacccag cgccccatcg
     1861 tattgcggtg cagctccacg ccctggcggg catagatttc cgactgacgg tacagcggca
     1921 gatgctcggc gaacttagcc atgattatgc gggccagcag agccggactg gcgtaactgc
     1981 gctcgatggg ttttggtggc tgcggagcct gaactataca gtcgcaccgg ctgcaggccc
     2041 gttttgggcg aaccgtttcg attaccctga acgcggtgtt gatgatatcc agttgttcag
     2101 agatgctttc tcccagcggt ttcagtttac cgctgcagac ggggcattcg gtttctgccg
     2161 gggagataac ctgcctgtca cggggaagtg ttgccggaag tgctttgcgg acgggagagt
     2221 ctgatgtttt cggcgctgtc tctccggcca ttgaggtgag ttgcaactgc gcctcaccaa
     2281 gcctgttctg gagctcggtt atacgcgttt ctgcccgtgc gatcttcttt tctatcttct
     2341 cgcggctttt ctcgctgctg cgaccgaaca acattctctg cagtttagcg accagcgctc
     2401 tgagtgagct gatctcgcgg gcatagccgg ttatttcacc agacagacgg acgataacag
     2461 cctgctgtgc gatcagcagg gccttcagtt gctcgatgtt gtcggggagt gtgttgttca
     2521 ttcccctgtt ttatcacggg ttatatccgg atgccaggcc gttctgtccg tttgggatgt
     2581 tgccatgcga tcccctccag tagcatggat aactgagctg gcgtcaggtg cactttccct
     2641 tcccgggtta ccggccagac gaagcggccc cgttccaggc gtttggcgaa caggcataac
     2701 ccgtcacgat cggcccacag tattttcacc attttgccac tgcggccccg gaagacgaag
     2761 atatgcccgg agaacggggc atctttcagc gtgttctgca ccttcgaagc caggccgttg
     2821 aagccacaac gcatatctgt gatgccagcg atgatccaga ttctggtacc ggttggcagc
     2881 gttatcatcg ggtacctcct ttcatttcgc ggattagcgc ccgtaacatt tccggagtga
     2941 gagggtcaaa cagttttacc acacctgatt taagatgcag ctcgcaccgt gggacgtttc
     3001 cgggatcact ctcagggcac tcatcaggct tgttacgcca gaagggattt gtaactggtc
     3061 tggtcggctc tggcgtatca gtcagagcca ccgggacagg catgcattcc tgtatgtcat
     3121 catcgctcag taagccgtcc tcgtactggc ttttccattt taacagcagg ttatcattga
     3181 taccgtgctc tctggcgatc cgggcaacaa cagcaccggg ctgtaatgcc tgttttacca
     3241 gcctgatttt aaattcacga ctgtaggagg ttcgtcgttc ttttcgccat gtgccttcgc
     3301 tgatttgagg ctctgttaat tccttctttc tgttggcata aaggatggcg tcaagctgag
     3361 ctaatgaaac tgaatcgggc aatggccatg cgataccgga tgcaataaat cgctgaaaaa
     3421 gcgtatgtat tgtggaatga ctgagaccta gacgctgagc gatggcccgg atggtcagtt
     3481 tatcttcaaa tcttaaacgc agagcatcag gcaaataaga acggaagcag ggaatatctt
     3541 tttttgtctg ggaattcatc gttcgtgtcc atctatatag atgggcgcga ttgttgccag
     3601 acaggacaat tttcacaaga cgtcgcagat ggggcgctta cacaacaatg gcgcgaggga
     3661 aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct cgatgcagct
     3721 gccgaacagc cagaatcgct cgcagaggac ccggaaaccc tcagtatcag ctgttgtcaa
     3781 cgacggatga ctattgcacc gttcgtgac
//
LOCUS       DBFIFS010000304         3801 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.305, whole genome shotgun sequence.
ACCESSION   DBFIFS010000304 DBFIFS010000000
VERSION     DBFIFS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.305"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(17..430)
                     /gene="hiuH"
                     /locus_tag="N7A07_004554"
     CDS             complement(17..430)
                     /gene="hiuH"
                     /locus_tag="N7A07_004554"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU2479067.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            563..1234
                     /gene="hprR"
                     /locus_tag="N7A07_004555"
     CDS             563..1234
                     /gene="hprR"
                     /locus_tag="N7A07_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU2479068.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1234..2592
                     /gene="hprS"
                     /locus_tag="N7A07_004556"
     CDS             1234..2592
                     /gene="hprS"
                     /locus_tag="N7A07_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU2479069.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(2700..3551)
                     /gene="hchA"
                     /locus_tag="N7A07_004557"
     CDS             complement(2700..3551)
                     /gene="hchA"
                     /locus_tag="N7A07_004557"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU2479070.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
BASE COUNT         1072 a          745 c          894 g         1090 t
ORIGIN      
        1 tttacatatt tatgcattaa ctgccacgat aggttgaata cccatattgg ctaagtaata
       61 aaggcacatg ataatgctcg ttcactttat taatatgaaa ttcaacgggg atctcaggga
      121 agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg acacggtaat
      181 cgcccgtagt tgcagtttgc tcgggccaca gtgccttaat tcgtccatcc ttatctgttt
      241 tggcggtatt aagttgtaac cagccgttgt ccgctttctt ttcaagagtg actgtcacgt
      301 cggcagcggg ttttcctgtt tgctggttca aaatgtgcac gctaagaatg ttttgttgtg
      361 cagcatagac caaagaaggt aatgaaaatg cagccgttac tacggagagt actaaataac
      421 gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg aaaaagcgtc
      481 cagggtaacc ttacagcagc attacaattt tgtaatgaac caggctgttt ctatgacata
      541 tgatttatgg catattattt tcatgaagat tttacttatt gaagataatc aaaggaccca
      601 ggagtgggta acgcaggggc tttccgaagc gggttatgtc attgatgccg tttctgatgg
      661 cagagatggg ctttatcttg cgctgaagga tgattatgca ttgatcattc tggatattat
      721 gcttccgggt atggatggct ggcagatctt acaaacgtta agaacggcaa agcaaacccc
      781 tgttatttgc cttactgcaa gggattctgt cgatgacaga gtcagagggc tggacagtgg
      841 agcaaatgat tatctggtaa aacctttttc attttctgag ttgctggcaa gggttcgggc
      901 acaattaagg caacatcacg ctttgaattc aacattagaa atcagcggct taagaatgga
      961 ctctgttagt caaagtgtga gcagggacaa tatcagtatt acactgacgc gcaaggagtt
     1021 tcagttactt tggctactgg cctccagagc tggcgaaatt atacccagaa cggttattgc
     1081 gagtgaaatt tggggaatca actttgatag tgataccaat acggtggatg tcgccattcg
     1141 caggctccgc gcaaaagttg atgatccttt tcctgaaaag ctaattgcca caatccgtgg
     1201 gatgggatat tcgttcgtag cggtaaaaaa ataatgaaaa gattatctat aaccgtccgt
     1261 ttaaccttgc tttttatatt gctgctgtct gttgctggcg ccggaattgt ctggactctc
     1321 tataatggcc tcgcaagtga gttgaaatgg cgcgatgata caacactcat taaccggaca
     1381 gcgcagatca agcagttatt aattgatggg gtaaatccag atacgttacc tgtatacttt
     1441 aaccggatga tggatgttag tcaggatatc ttgatcattc atggtgatgg catcaataaa
     1501 attgttaacc ggacaaatgt cagtgatgac atgttaaata acatacctgc tagtgagaca
     1561 atcagcgcag ctggcattta cagaagcatt attaatgata cagagataga tgctttacga
     1621 attaatattg atgaagtttc gccatcatta acggttactg tggctaaatt ggcttcagcc
     1681 agacataaca tgcttgaaca gtataaaatc aatagcatta taatttgcat tgtcgccatt
     1741 atactgtgct cagtattaag tccgctgtta atccgaacgg ggttacgaga gatcaaaaag
     1801 ttgagtggtg taacggaagc gctgaattat aacgatagcc gggagcctgt tgaggttagc
     1861 gcattaccga gagaactaaa acctcttggg caggcgttga ataaaatgca tcatgcttta
     1921 gtcaaagatt ttgagcgtct aagtcagttt gctgacgatc tcgctcatga acttagaacg
     1981 ccaattaatg cattactggg tcagaatcag gttacgctca gtcaaaccag aagtatcgct
     2041 gaatatcaaa aaacaattgc cggtaacatt gaagagctgg aaaatatttc gcggttaaca
     2101 gagaacatac tgtttcttgc cagggcagat aaaaacaatg ttttggtgaa actggactcg
     2161 ctttctctca ataaggaagt cgaaaatttg ttggactatc ttgaatatct ttcagacgag
     2221 aaagagattt gctttaaggt cgagtgcaat cagcaaatct ttgcggataa aattttactg
     2281 caacgaatgt tatcgaatct tattgttaac gctattcgat attcgccaga aaaatcgcgt
     2341 attcatataa ccagttttct tgataccaac agctatctta atattgatat cgccagccct
     2401 ggagcgaaaa ttaatgagcc tgaaaaactc ttccgtagat tttggcgggg agataattcg
     2461 cgtcattccg taggtcaggg acttggtctt tctttagtca aagcgattgc cgaattgcat
     2521 gggggaagtg ctacgtatca ctatctcaat aagcataatg tgttccggat tacgttgccg
     2581 caaagaaatt aacatagggc ttcagtacgc cataaattaa ccgtgaaaaa acgcagccac
     2641 gtcgtgagta ctaacgcggc tgcgattgat tatgcgctta cattcaaacg taacagggat
     2701 taacccgcgt aagctgccag catttcctgc gccgccagtt tacccaacgc attcgctgca
     2761 aaaggactgt cgccggtgag aagtttacgg tccttatgta ctcgcccggt gatgtcgtca
     2821 ttaatgatgt tcatgcccat tttcttcagt tcttcgccga agtaccaggt gagatgaccc
     2881 ggcatatagc caatctctgg cgtttgtttg tctgcggcgt ctgggaatgc gcaaatggaa
     2941 taaccattca gtggattatc gccgtggcga agcgccagaa aagccgccgg gccgtggcaa
     3001 agggagataa caaaacggtc atttttgatt gcccactgta aagcggcagc tacgtcctgg
     3061 ctttcaggta aaccaataag tgcgccatga ccaccaggaa caaagattgc tgcatattcg
     3121 ctatcagcgt tgaggctggc gacaacatcc gcgagtttct tcggattgcg gaacaacgat
     3181 ttatgctgct caaagaatgg cattactttt tcatctttct gcggcatagc ccagtattca
     3241 aacttggtca tcagaccgga aatggtcgcc acttcgaatt cgaaacctgc agcatggaga
     3301 tgatacaacg gcagcaacgt ttcaatcgga tggttaccgg tcgagaacag ttttccgtta
     3361 tcggtcggca aataacgttc gtccgcggcg atcaccagaa ttttatgttt accgcgataa
     3421 ggttttggat agtccacgcc atcaagatca gagacaggac tggtatattg gctaagcgaa
     3481 tattctgaag ggaagaatgc attatcttca gcaatatcga cctgcggatt tttactggtt
     3541 tgaacagtca tagtgtattc cttattgttg cttagttagg gtagtcacta tatttgcgac
     3601 tgagctggtc gctattttta accaggctgg aaaattgagt caatatatcg aaagcggtag
     3661 tcggcgggtg taagtatgta atgatgatgc tgcactggtg ttgaatatta ttcacgccaa
     3721 attttcctta ttgagcaact tcgtcgcaag tggcctcaat gattttagac cattatcaga
     3781 ggtacggggc ggcggggaga g
//
LOCUS       DBFIFS010000305         3688 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.306, whole genome shotgun sequence.
ACCESSION   DBFIFS010000305 DBFIFS010000000
VERSION     DBFIFS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.306"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1156)
                     /gene="rhsD"
                     /locus_tag="N7A07_004558"
     CDS             complement(<1..1156)
                     /gene="rhsD"
                     /locus_tag="N7A07_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HGU2479071.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLPAAPLVRYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSY"
     gene            complement(1223..3331)
                     /locus_tag="N7A07_004559"
     CDS             complement(1223..3331)
                     /locus_tag="N7A07_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2479072.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          762 a         1117 c          972 g          837 t
ORIGIN      
        1 ggtaactcag gcctgccggg ttcagttgct ccaccacccg ccccgcatcg tcgtagcggt
       61 agcgcatctc cggccttccc gcataacggt gccccaccat ccggcccggg tgctgcgcgt
      121 catacgtgaa agcgcgcacc tgcgtattgc tgcggtcata taccgccagc agttcaccgg
      181 cttccgtata cgtgtaccgc accagtggcg cagcgggcag gctctccggg tatgccgggt
      241 cgtgcgtcag ccacaccgcc gaaaggcgga tacctctgtc gggaccgtat tcggtaccgg
      301 gcagtgtgtc ggggaacgct gagtctgaaa gagggcgggg ggtgtcaggg gaagataaag
      361 aagcggtgtg ctgtttacgg gcctcttccg cccgctgcgc ctgcgtggtc agcaccagac
      421 ggaactcccg cccggcaccg tccgtcacgc cggtgatttc cccggccagg tcaccggcgg
      481 cctcacgcct gtacgtcagc gtccgcccga agcggtccgc catcccggta agcacccggt
      541 acggcggcag cggcgctggc agtacgtcct cagcacccgg cacccgctct gaccacccca
      601 gtatccacca cggcccctgt gcgctgttgg tcgccaggta aagatgcggg cttaaccgga
      661 tatccggcgg cagcgccccc cacagccgcg ccagcgtgtg gccgtccggc tgcgctgcct
      721 taccaccgcg caccagccac attgactcgc tgcggctgta caccgcctcc cccggcagca
      781 gcggctcaaa gtgaatgctc cgcccgccgt tgtcattgag taccagtgca tcatcgcgta
      841 gctgtaagcg gatatcagaa ggcgctttcc agccggggcc aaaaatcccc accggcgcag
      901 gcgtccgggt ccggtagctg ctgtaggtgc gggagagaat gaacggcagc gggccgggca
      961 gcgcaaggtc cgtctcgccg ggcagcacct tcgcccccag cagcggattt accgggttgc
     1021 ccgaagtcat cccgcccggg cacaccgagc acgccacgcc agttggcgcg ccaattctta
     1081 cacctgccga accctggaca atgggaccgc catactgagt catatctccc tgacgcgccg
     1141 ctggttttcc gctcatgatt caattccttc ttctgtttat ttttaatgct tgtttgttac
     1201 tgaataccga acaggtacgg gatcagtact ctctcatatc cggcgtatcc tgcttttcat
     1261 caggggtctt cggtggggat ttcgaggctg cacagttcca gttaatcaac agtgagtcac
     1321 cattaacctg ctccttaccc tgcaaatgaa tttttgtgcc gttgagaaaa atttgtccgt
     1381 ccttcgtcag caccagcctt gccttaccac agcagagctc cagatgctca ccggcggagt
     1441 aaatcgcggt ctgcccggta tcatcctttt tcgttttacc aacggtgaag gtaacgttat
     1501 ttccgacttt gacgtcataa cccagcccga ccctcaacga tttatgcaaa ccgatctgcg
     1561 aggactgcat cagagccacc gaagtgttca taatgccacc taccgtcgtc tggtacgcca
     1621 cgcccacggt cagcgcacga acaagtccaa tcgtttccac ctgaacagaa cccacgttaa
     1681 tgatctggtt actgcccacc gtctctatct ggttaacgcc caccgtctgt atctgattgt
     1741 tggtaaccgc aatcatctga ttgttgccaa tggtctcagc atggttgttt atcacatccg
     1801 tggtgcggtt attcagcacc tcggtgttca tgttcttctg cgcgtggatg tagacctgtt
     1861 ctttccctgt cgcatcgtca aacttcagtt cattaaaccc gctgccctta tagtttttcg
     1921 aacgaatggt catctgcgtc ttcgtccccg gcaggctgcc gggtgtgcgg ttttcctggt
     1981 ggtaggtacg ccccataata atcggctggt ccggatcgcc gttgaggaag tccacaatca
     2041 cctcctgacc cacacgcggt atcgccaggt tgccaaagcc ggtgcctgcc cacgcctgtg
     2101 ccacacggat ccagcatgaa ctgtcctggt ttgacgggtt atagcggtcc cagttaaatt
     2161 tcacccgcac gcgaccatgt tcgtcgcaga agatttcctc gcctgccggt cccgtcacga
     2221 cggcgctctg cgggccatcc accagcggtt tcagcaacgg ctgtggtcgc catgtccggt
     2281 ctgccggtat taccgcaaag tggttattca gcgtggtacc tgaacccctg cgtcccggca
     2341 ccgcctgtgg ctgctcgccg tgcagttcac ttgccaccac ctgccattcc cggttcaggt
     2401 tcgcctgcgg atgccccgtc agcacaattc gccgtcccgg ccatatttcc ggagaacggc
     2461 ttgttccgcg cgccacttct gcgttgttgc gccagccatc catctgccag cgggcaaagt
     2521 tctgcccgtg ggcacccttg aaacgtccgg ggtagtcata cacttcatac tgtgtgcgct
     2581 ggtaatcctg gtactggcct tcctgatcaa aacgccctgc ccagccgggt cgtttaaagg
     2641 tgtagtcctt ggtcaccacg gaagaagggc ggatttgtgc gctgtagcgg aactggctga
     2701 tgcatagggt gctcacctcg gtacgggtgt tcgggttcca ggggatctca aaggactccg
     2761 gcagataacg cacggtgtcg cacaggacca ggctctggtc gatacttttt tgcgcgtgct
     2821 cctcataaaa gaagatgcct tcctccgccg ccatccggca caggaaatcg taatcagttt
     2881 cgccgtactg gacacaaaac tcacgggaag gatgcggctc gctgaacagc ggactccact
     2941 cggtcacacc gttttctttc aggatcgtag cgagtatgct ttcgatgtcc tcattctgga
     3001 agatacggaa gttctggcgc agccctgtgc gccacagcgg cgggcacacc ttcatgctgt
     3061 acagcatctg gtttttgtcg ttctccccca gttcaaacca ggtcaccaca cctttcaccc
     3121 ggcgctgtac gtcatcgccc tgccataccg tcaggtaggc cattttgtcc agcacctgcg
     3181 caaattcaag ggaaagaaac tgctgactga ccagagaaag gtcgagggaa aaaagtgaag
     3241 agagtgactg gttcagatga aaggagacca ccgcaaacgc gtccggtggc aggccgtcca
     3301 cttccagcgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
     3361 cgcacatatt gcatgtacaa catgtcgggc ggatgaaata agtgagtcga catctggcgt
     3421 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
     3481 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
     3541 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
     3601 tcaaaccaat attcataatg tctttttatt cacaacaata aataatttaa taacaatcat
     3661 tgaaatgaat gatgatttgt ataaggaa
//
LOCUS       DBFIFS010000306         3687 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.307, whole genome shotgun sequence.
ACCESSION   DBFIFS010000306 DBFIFS010000000
VERSION     DBFIFS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.307"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..533
                     /locus_tag="N7A07_004560"
     CDS             <1..533
                     /locus_tag="N7A07_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU2479073.1"
                     /translation="FMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKDTPSFGHSCRHS
                     MCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKSYRISRQLGDIW
                     RRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILR
                     RSRLPEKSNRGRPRHN"
     gene            688..3048
                     /locus_tag="N7A07_004561"
     CDS             688..3048
                     /locus_tag="N7A07_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU2479074.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3210..3386)
                     /locus_tag="N7A07_004562"
     CDS             complement(3210..3386)
                     /locus_tag="N7A07_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479075.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3524..3687)
                     /locus_tag="N7A07_004563"
                     /pseudo
     CDS             complement(3524..3687)
                     /locus_tag="N7A07_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          951 a          769 c          953 g         1014 t
ORIGIN      
        1 acttcatgat tctgaaagac gtgaatgaac acatggaagg tctgtttcaa cgtttttcta
       61 aactgttacc tttcagaatt gattttgcct acaggaaaga cacgcccagt tttggtcatt
      121 cctgtaggca cagtatgtgt attgaaatgc accgcttact tagtgaaacc caaacgatgc
      181 tggcgggata ttactgggta atggaatata cgtcaaacaa gggacttcat attcatttta
      241 tcggctacct ggatgggcaa cgtcacaaca aatcttatcg gatatccagg caactgggag
      301 atatctggag gcggatcacg gaaggggagg gttattttca cctgtgcagg gctaaagaca
      361 aatacccggt gcgtatcgat cacgtaattc actatagtga taaatctgcg gtagacgact
      421 tacgttacgc ccttagctat ctagccaaac aggatcagaa agaacacggt attattctga
      481 ggcgtagcag gcttcccgaa aaaagtaacc ggggccgacc acgacataac tgaattacag
      541 ctgtattagg tattagaatt aagtttctgt aagttgttgg gaggtgcttg agttaatagg
      601 cagaagaggg cggggcgctt tgtcatcagt gtaggggacc cgaatttctg ccgtggtgcc
      661 tcagtggatc ggatacaggt aacgaatatg gttcataaat ctgacagtga tgaattagct
      721 gcattgaggg cagaaaatgc ccgtctggtc tcattacttg aagctcatgg gattgaatgg
      781 cgacgtaaac cgcagactcc tgtgcagcgc gtttctgtat tatccaccga cgagaaggtt
      841 gcattatttc gtcggttgtt tcgtgggcgt gatgatgtat gggcacttag atgggaaagt
      901 aaaaccagcg gcaaatcagg gtactctcct gcctgcgcta acgaatggca ggcgagaata
      961 tgcggtaaac cccggataaa atgtggggac tgcgctcacc gtcagttgat tcctgtatcc
     1021 gatctcgtca tctaccacca tctggccggt acccatactg tcgggatgta tccgttgctg
     1081 gaagatgatt cctgttattt tctggcagtt gattttgatg aagctgaatg gcagaaggat
     1141 gcatccgcat tcatgcgatc ctgtgatgag ctgggtgtac ctgctgcgct ggaaatatcc
     1201 cgttcacgtc agggggcgca tgtctggata ttttttgcct cacgagtttc ggcccgcgaa
     1261 gctcgccgtc tggggactgc tattattagc tatacgtgta gtcggacccg acagctgcga
     1321 ttggggtctt atgaccgatt atttcctaat caggatacta tgccaaaagg gggatttggt
     1381 aatctcatag cgttacctct gcaaaaaaga ccgcgtgaat tggggggaag cgtttttgtt
     1441 gatatgaatc tccagcctta tcctgatcag tgggcttttc ttgtatcggt gaccccgatg
     1501 aatgtgcagg atattgaacc gacgatatta cgggctacag ggagtatcca tcctctggat
     1561 gtgaatttta tcaacgaaga agacctgggt acgccgtggg aagagaaaaa atcatcagga
     1621 aacagactga atatttctat tgcagaaccg ctgaaaatca cgctggcaaa ccagatctat
     1681 ttcgaaaaag cgcaattacc tcaggtgctg attaaccgac ttattcggct ggcagcattt
     1741 ccgaaccctg agttttataa ggctcaggca atgcgtatgt cagtctggaa taagccccgt
     1801 gttataggtt gtgcggagaa ttacccgcaa cacattgcgt tgccccgggg atgtctggac
     1861 agcgtattat ctttccttag ggacaacaat attgctgcag aattaatcga taaacgattt
     1921 gccgggacgg aatgtaatgc cgtttttatg ggaaacctca gagcggagca ggaagaggcc
     1981 gtttcggcat tactccgtta tgacactggt gtgctttgtg cgccaacggc ttttggtaag
     2041 acagttaccg cagcggcagt gattgccaag cggaaagtga atacactgat actggtacac
     2101 cggactgaat tgctgaagca gtggcaggag cgtctcgcgg tgtttctgca ggccggtgac
     2161 agtattggta ttatcggggg aggtaaacat aaaccctgtg gcaatattga tattgcggtg
     2221 gtgcagtcca tatccagaca aggagaagtt gaacctctgg tcaggaatta tgggcaaatc
     2281 attgtggatg agtgccatca tattggcgcg gtttcatttt ctgcgattct gaaggaaacg
     2341 aatgccagat atctgcttgg cctgacggca acaccaatcc gacgggatgg tctgcatccc
     2401 attattttta tgtactgtgg tgccattcgc catacagcgg tccgcccgaa ggaaagccca
     2461 cataatctgg aggtactgat ccgttcccgt tttacatctg gtcatttacc atcggatgcg
     2521 agaatccagg atattttcag agaaattgct ctggatcatg acagaacggt ggcgatagct
     2581 gaagaagcca tgaaagcttt cgggcagggg cgaaaagttc tggtactgac tgaacgtaca
     2641 gatcatctgg atgagatagc atcagtgatg aattcactga aattgtctcc ctttattctc
     2701 catggtcgac tatcgaaaaa aaagcgtgcg atgctgatat ccgggctgaa tgctcttcct
     2761 cccgattctc ctcgaatttt gttgtcaaca ggcagactta ttggtgaggg atttgaccac
     2821 cctccgctgg atacgctgat tcttgccatg cctgtgtcat ggaaagggac attacagcag
     2881 tatgcagggc gtcttcacag agagcatacc ggtaaaagcg atgtcaggat cattgatttt
     2941 gtggataccg cgtatcctgt gctgctcaga atgtgggata aacgtcagcg gggttataaa
     3001 gcgatggggt acaggattat agctgacggc gatgaatcag tgatttaatt ttaaggagta
     3061 ctttaccctt tttaaataat ttgagctccc cgggttgttc gggcaacaaa gagtcccagt
     3121 atacaggctt caggaatatg ttgtagtatc aggcttttca tcgctttaaa atgactaccg
     3181 gcagtgagaa cgtcatcaaa aataacaatc tagggcctgc acccttccag taattgttca
     3241 tccagttggt agtttgccat aatcgcttct ggtaagggcc ggtttgttgc ggtatgagag
     3301 gctattgttg atacgttttg cacaaccagt tcccagaaat ccagccatgg ttcgtaggtt
     3361 tgcgcaattc gcagaatttc tgtcatacgg tcatcgtaag cggcatctgt acggcatttg
     3421 gatggtggta cgggaacaaa tgtgtattcc tcaagattac cgatatttga tatgagcatc
     3481 tgagcaacat actcaatagc agccttttta tagtggtaat caggctacga aagtacagcg
     3541 ttttataaat tgtctcaggt gatattcgca gcgttttttg acgtggtttt gttcgcctta
     3601 accatcctga tatttgctct ggagaccatt tcatctccag cttttccaga acaagctttc
     3661 gcaatggtaa attttgatcc agtaagc
//
LOCUS       DBFIFS010000307         3629 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.308, whole genome shotgun sequence.
ACCESSION   DBFIFS010000307 DBFIFS010000000
VERSION     DBFIFS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.308"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..885)
                     /locus_tag="N7A07_004564"
     CDS             complement(<1..885)
                     /locus_tag="N7A07_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGU2479076.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDA"
     gene            complement(895..1575)
                     /locus_tag="N7A07_004565"
     CDS             complement(895..1575)
                     /locus_tag="N7A07_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU2479077.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1562..2029)
                     /locus_tag="N7A07_004566"
     CDS             complement(1562..2029)
                     /locus_tag="N7A07_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGU2479078.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2029..2565)
                     /locus_tag="N7A07_004567"
                     /pseudo
     CDS             complement(2029..2565)
                     /locus_tag="N7A07_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2629..3276
                     /locus_tag="N7A07_004568"
     CDS             2629..3276
                     /locus_tag="N7A07_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2479079.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT         1021 a          857 c          837 g          914 t
ORIGIN      
        1 agcatcacct aaaagtatca taactttatt tgatattatt tttgaaataa taccaccatt
       61 aaacgaaaac actaatattg aaaaggccat tgatggtata aaaagcgcat catactgtaa
      121 tccattgcta ttttgacttt ttgccaccat atagacagac attaggataa agactacaga
      181 tgatatttca gcaaatgata gaactaaata tgacggcttt gacttaaact caaaatctcc
      241 ggcctgattg ctgcggattg tctctgccag cctgcctcgg tcaatctcac gaccagccat
      301 cttgtcctga acattgaagt agtcaatcgg accgagagca gccatcccaa ggtgataaac
      361 aaactcacca aaccctgaag gattctgctg atacatctga gcaacgttgt tagggtcaac
      421 accgacgcgc gccagttcct tggcgttgtt ttgcagccat gattgcattg cttctggaga
      481 cgaggccgca aggcgtgcgc cagccgctaa ggtgccgata gaattgcgct ggtcttcgtc
      541 tgcccacttc ataccagact gaatcttctc taattgacca ggatatttgg tcatcagatc
      601 tcgaacctgc tgtcgatcgc cggactggat ggctgccgca tattcttttt ggaatgcagc
      661 atccgcttcc tgttgctttg cggcttgaga tgtttgagcg acactaccaa gtccctgcaa
      721 cgcctgaagg ccgatgttat tgcgacctga acgctccatt tcgttgttct ggcgaatata
      781 ggccaacgcc tcacttacat cacttgcctt tggcgcattt gagttttgcc caccgatacc
      841 agcaagaaaa ccgcctgagt tgattccttg ttgccaagta gccatattcc caccttaaaa
      901 caatgatcca agaccaccga taacaccgcc agcaagagca ccaataccag tcccaataac
      961 aggaactgca ctaccaatca ttgcacctga tgcggcccca ccaatggcac ctgtagcaaa
     1021 actctgtagg cctgaaggct tattcgcatt tgctgccgat gctgccgcct gctgttgata
     1081 caattggctg acattgttag cgtagttctg tccggcgttt gcctgacctg taagagcacc
     1141 aaggccgata tttgccagat tgttgtagtt gttcatctga cctgacagcc agttttgacc
     1201 gagtgtaggt gcgattgctg ctaactggtt tcctgttgct gtagagccta atccacccgt
     1261 tgcctctgct gctgccagac tctgatagcg cgcctgccct gcaaggtctt tatactgctg
     1321 agagttgtaa tactggttaa gcgcctgacc ttgcccctga agagaggaaa gattctgcaa
     1381 ctgtgatacg tactgctgag cgagtggcgt gaacggtgca aggttttgca tgttcgtctg
     1441 ccacatttca cgctgcagtt cgatgccctt ttcagttgcg cgtgcctggg ctttagatcc
     1501 ggagtcgctg ccgcctttca tatacccatt catgggaagc aatttattct tgaagctttc
     1561 gctaagtact aacatttaat agctcctcat atttcgaacg aggtaattga tagagggtga
     1621 ttcctaccgg ttttccgtta ctcatgtacg catcatcaag gtgaccaaca cgggtagcgc
     1681 caagcaaacg gataattgcc cgtccgtatt tggtggtgtc aggaaccatg gtgatgctgt
     1741 taaggaatgg tgagttttcg agaagccatt tgcagaataa tcgatgccct tgtagtgcat
     1801 attcgccacg gaatccgggg tcatacaccg catggcattc aacaacgcta tgccagaagt
     1861 tacgcacttc atgaactcca gccagcacta atccttcgta gatgccgaga tataccgcat
     1921 caggcttgat gtagtattta tctccactgt ctacgatatt ccccgtgttt accgggttgt
     1981 taaggaattc tgcaagtttc accgggttat cgatgagctt tatttccatc actgctccgc
     2041 aatgattttg atggttgtgg cagtaaacgc cgcgccattc gactgaatgg ttaacgtgct
     2101 gccatttgtg gcaagaaagc cgtctttatc cacgctgaag aacgtagcta acaggatgtt
     2161 gtcggttgtt gttgccgagt tgcgactgct tactagcgtg tcaggaacag aaccggagaa
     2221 tgttagctgc attgacctgt tggcggttcc gctgggccac gtcccgacaa tcgacagctt
     2281 gaagaacaag gttttgttct cgttgaacac aaccatcttg ttgttaacgg tgtcgaagaa
     2341 tggtgccaac gtcccggatg acggcgtaag cgttttcagc aggctaacaa ggttggtcgg
     2401 cgctgtcggg atggttactg atacgccaga gtaaacaacc tctgacttct tgcgcgtagt
     2461 ggcatactcc agagcattaa tgcgcgtttc atggtctgaa acctgcgatt ccagcgactg
     2521 aactctggta tcaagcgatg caatatcgct ttcattcaag gcaatcagtg ggtttacgtc
     2581 atggagtggg ctcaacacca gtcagagccg catgtacacg caataatcat ggtgaacgaa
     2641 agaagcatta atgggcaagc tggcaaaatt gtcagcaaat gggtaacact ggcaaacaat
     2701 gcaatcccat caaatgttcc tccccagtgg gatctagatt tgaaaggtca gcaatattca
     2761 gagcctacaa agtgtatagg taaacagatt gattacatgg caaagcccga aactaagtta
     2821 aatgacttta atagagaggc gagcaagagc ctatatgatt ggtcagatgc caacgtttgg
     2881 ggttattcga atggctgggc gcggcatgag ataaaaaaag aaacactttc tatcactggc
     2941 tttcatgctg taagacgcat actaaaagcc gttcaggcat caaggaaaga caactacaaa
     3001 gctaagcgac agatacagcg tacattgaaa cgacaagaca taaatcgatc accaatcacc
     3061 ccgttccaag caccgttaat tagcaaacac gattgggatt tatcagtgat agcggtcacc
     3121 aaagaggcag agacagagcg cagggaatta ctatcctcac agataataaa ctcgaacaaa
     3181 gtacaaaccc acccactgtt aaggatgtta agaagagcac gagtgagagc atccagagcc
     3241 aaaagagaaa gcggaggtta tgaccattac tactagtagt gacagatcgt gacaaggcgg
     3301 tgcaaaaaac gaccaataat aagagagata tgcctgccaa accgccaaaa gcgcggagcg
     3361 cggcaaacgc cgcgagaggc gcaagaaggc gaacctatcc attgaaacgg tcagaagcgc
     3421 atcgcgctcg acgaccgaag tccccactaa caccgttaac gaaagatcaa agtcttagaa
     3481 aaaaatgacg tttctttgat ggacttccat ccgtaaaaat caaattttga gaaaaagtac
     3541 ttatagaaat taatcgctct ataaggataa gttgacgtta gtttaaagtg ataaaacatt
     3601 aaagaaaaaa cgtaaaatca aatacataa
//
LOCUS       DBFIFS010000308         3584 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.309, whole genome shotgun sequence.
ACCESSION   DBFIFS010000308 DBFIFS010000000
VERSION     DBFIFS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.309"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            29..679
                     /gene="csgD"
                     /locus_tag="N7A07_004569"
     CDS             29..679
                     /gene="csgD"
                     /locus_tag="N7A07_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU2479080.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            684..1073
                     /gene="csgE"
                     /locus_tag="N7A07_004570"
     CDS             684..1073
                     /gene="csgE"
                     /locus_tag="N7A07_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU2479081.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1098..1514
                     /gene="csgF"
                     /locus_tag="N7A07_004571"
     CDS             1098..1514
                     /gene="csgF"
                     /locus_tag="N7A07_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU2479082.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1541..2374
                     /gene="csgG"
                     /locus_tag="N7A07_004572"
     CDS             1541..2374
                     /gene="csgG"
                     /locus_tag="N7A07_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU2479083.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVTQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2438..2929)
                     /gene="ycdZ"
                     /locus_tag="N7A07_004573"
     CDS             complement(2438..2929)
                     /gene="ycdZ"
                     /locus_tag="N7A07_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU2479084.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3031..>3584)
                     /gene="ycdY"
                     /locus_tag="N7A07_004574"
     CDS             complement(3031..>3584)
                     /gene="ycdY"
                     /locus_tag="N7A07_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU2479085.1"
                     /translation="NEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQD
                     ELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPLA
                     DTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFWR
                     TMAPLTRDAISAMWDELEEDSEE"
BASE COUNT          997 a          878 c          822 g          887 t
ORIGIN      
        1 cgatcaataa aaaaagcggg gtttcatcat gtttaatgaa gtccatagta ttcatggtca
       61 tacattattg ttgatcacta aaccttcttt gcaggcgaca gctctcttgc agcaccttaa
      121 acaatcgctg gcaattacag gaaaattaca taatattcaa cgttctctgg acgatatctc
      181 ttcaggctct attattcttc tggatatgat ggaagcggat aaaaagctta tccattattg
      241 gcaggatact ttgagcagga aaaacaacaa tatcaaaata ttgttgctaa atacgcctga
      301 agattacccg taccgcgaca ttgaaaactg gcctcatatc aacggcgttt tttatgccat
      361 ggaggatcaa gaacgcgttg tcaatggatt gcaaggcgtc ctgcgcggcg aatgctactt
      421 tacgcaaaag cttgccagtt acctgattac acattctggt aactatcgtt ataacagcac
      481 ggaatcagcc ctccttactc atcgggaaaa agagatcctg aataagctgc gtatcggcgc
      541 gtctaataac gagatcgctc gttcgttgtt catcagcgaa aatacggtta aaacgcatct
      601 ttataatctt ttcaagaaga tagccgtaaa aaaccggaca caagcggttt cctgggcaaa
      661 cgataacctc aggcgataaa gccatgaaac gttatttacg ctggattgtg gcggcagaat
      721 ttctgttcgc cgcagggaat cttcacgccg ttgaggtaga agtcccggga ttgttaactg
      781 accatactgt ttcatctatt ggccatgatt tttaccgagc ctttagtgat aaatgggaaa
      841 gtgactatac gggtaactta acgattaatg aaaggcccag tgcacgatgg ggaagctgga
      901 tcacaataac ggtcaatcag gacgttattt tccagacttt tttattcccg ttgaaaagag
      961 acttcgaaaa aactgtcgtc tttgcactga ttcaaactga agaagcacta aatcgtcgcc
     1021 agataaatca ggcgttatta agtacgggcg atttggcgca tgatgaattc taaataaaaa
     1081 attgttcgga ggctgcaatg cgtgtcaaac atgcagtagt tctactcatg cttatttcgc
     1141 cattaagttg ggctggaacc atgactttcc agttccgtaa tccaaacttt ggtggtaacc
     1201 caaataatgg cgctttttta ttaaatagcg ctcaggccca aaactcttat aaagatccga
     1261 gctataacga tgactttggt attgaaacac cctcagcgtt agataacttt actcaggcca
     1321 tccagtcaca aattttaggt gggctactgt cgaatattaa taccggtaaa ccgggccgca
     1381 tggtgaccaa cgattatatt gttgatattg ctaaccgcga tggtcaattg cagttgaacg
     1441 tgacagatcg taaaaccgga caaacctcga ccatccaggt ttcgggttta caaaataact
     1501 caaccgattt ttaagcccca gcttcataag gaaaataatc atgcagcgct tatttctttt
     1561 ggttgccgtc atgttactga gcggatgctt aaccgccccg cctaaagaag ccgccagacc
     1621 gacattaatg cctcgtgctc agagctacaa agatttgacc catctgccag cgccgacggg
     1681 taagatcttt gtttcggtat acaacattca ggacgaaacc gggcaattta aaccctaccc
     1741 ggcaagtaac ttctccactg ctgttacgca aagcgccacg gcgatgctgg tcacggcatt
     1801 gaaagattct cgctggttta taccgctgga gcgccagggc ttacaaaacc tgcttaatga
     1861 gcgcaagatt attcgtgcgg cacaagaaaa cggcacggtt gccattaata accgaatccc
     1921 gctgcaatct ttaactgcgg caaatatcat ggttgaaggt tcgattatcg gttatgaaag
     1981 caatgtcaaa tctggcgggg ttggggcaag atattttggg atcggtgccg acacgcaata
     2041 ccagctcgat cagattgccg tgaacctgcg cgtcgtcaat gtgagtaccg gtgagatcct
     2101 ctcctcggtg aacaccagta agacgatact ttcctatgaa gttcaggccg gggttttccg
     2161 ctttattgac taccagcgct tgctggaagg ggaagtgggt tacacctcga acgaacccgt
     2221 tatgctgtgc ctgatgtcgg ctatcgaaac aggggtcatt ttcctgatta atgatggtat
     2281 tgaccgtggt ctgtgggatt tgcagaacaa agcagaacgg cagaatgaca ttctggtgaa
     2341 ataccgccat atgtcggttc caccggaatc ctgacagata aaaatgccgg aattaataat
     2401 ccggcattta ctgtttaagc gaaagcgtga gtgcccctca cgctttattt ttgatttcct
     2461 gctcacggag agccgtcttt gcactacgcg ccgccagcca caggccgctg tttttcattg
     2521 cgtaaccaaa tatcagccca agcgccagcg aaggtaacac cagtttccag tcaccctgcc
     2581 cggcaaacgt cgcgcatgcg cctataaacg tccccggaac aaatgaaagc agcagctgtt
     2641 tggcctgaat acacatcaga aacgcgacaa taccggttat gacataactg agaatttcca
     2701 gatgtggcgc cagtgcacta ccgtaaataa tgaccatcgc ccacaccacg ccactaagca
     2761 gagttgcagc ggagatcgcc agccctttca gcccaccttg cgggcaggca aagtaggccg
     2821 tacagcccag gaagcccgcc cagcttagta agcccaggga aacagccacc catccccaga
     2881 taccggagag aatgcctgtt gtgattgcaa tagagagaag tatgttcatg ccgcgcatga
     2941 tagcagaaaa cgcggccatg aatgagatcg agcacacatt ttatgcaaca gcaaattatc
     3001 tgttgcagtt atgagtgacg catatcacat ttactcttca gaatcttctt ccagctcgtc
     3061 ccacattgca ctaatggcat cgcgggttag cggtgccatg gtgcgccaga aaggcgtggt
     3121 tgcatgggcc tccactttgc caaggaacgc accacaccag ggtaacagat actcactgaa
     3181 cagtgtttcc agtgcttcgc tctcatcttc cgttgactga tcttccagcc aggaagccgc
     3241 aagcagcaat gtgccgatgt gatcggcagg cgtatccgct aatggcatcc ctcgttcaga
     3301 aagaaaagcg cgcacttccg cttccgttgc gtcctcaacc catgcgctac gatatggcgg
     3361 cacggcacat tcatcgccga taaacaacgc attgtaatcg gcagagactt gcgtcatatc
     3421 acagcttttc tgcaaacgtg tcagcaactc atcctgctcc agtggccagt tcgcagccag
     3481 tttcccctca cgaatcaggg taaacagcgg caccagtaaa ggatcttgcg gttggcggta
     3541 atagagcgaa cccagcacac gacagaggat agaaaactcg ttca
//
LOCUS       DBFIFS010000309         3553 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.310, whole genome shotgun sequence.
ACCESSION   DBFIFS010000309 DBFIFS010000000
VERSION     DBFIFS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.310"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1265)
                     /gene="escN"
                     /locus_tag="N7A07_004575"
     CDS             complement(<1..1265)
                     /gene="escN"
                     /locus_tag="N7A07_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU2479086.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQ"
     gene            complement(1249..3276)
                     /gene="escV"
                     /locus_tag="N7A07_004576"
     CDS             complement(1249..3276)
                     /gene="escV"
                     /locus_tag="N7A07_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU2479087.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3273..>3553)
                     /gene="cesL"
                     /locus_tag="N7A07_004577"
     CDS             complement(3273..>3553)
                     /gene="cesL"
                     /locus_tag="N7A07_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HGU2479088.1"
                     /translation="RVEIDFNGFSFFIEIIDNNVFVTFALEYNENAFFSFFSALAPER
                     TQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTFQQHVQIIERVRRMTS"
BASE COUNT         1125 a          824 c          651 g          953 t
ORIGIN      
        1 ttctgaatta atttcctatt ttttattgcc ttatctgctt caggatcttg ccccatcgtg
       61 tactcaccaa tacgaataag cagctcaaca tttttataag ttgcaataag ctttttgcat
      121 tctgccgccg cacgcaaatg ctccgatgta acaacgttat gcataacacg actggcactt
      181 aaaccaatat caattgcagg gaaatggttt tcctctgcaa gttctcgggt aagtacgatg
      241 tgcccatcaa gaatagagcg gacttcatcg ccaataggat cattaacatt atcgctttct
      301 aacaacaccg tataaatcgc ggttattgaa ccttttggcg caggccccgc tcgctctaat
      361 aatttgggta acgaagaaaa aacactcgga gggaatcccc cccttacatc aggttctccc
      421 gatgcaagcc caacatcacg tgcggctcgg gcataacgag ttacagaatc catcattaat
      481 aatacatttt taccttgatc gcggaagtac tctgcaatag tcgtcgccgt aaatgcggct
      541 ttcatccttt ccagcgcggg gcggtctgac gttgtgacga ccagtacaca tttagaaagc
      601 gtggattgag gtaagagcgc gaggaattca tttacttcgc gaccacgttc gccgataaga
      661 gcaaggacaa taatatctgc ggatgcgcca ttacaaatca tccccaaaag cgtactttta
      721 ccaacgcctg aaccagcaaa aataccgata cgctgcccaa taccgcaagt tagtagccca
      781 tcaattgccc tgacgccaag tataaatggc tgatcaataa cctgccttaa taaaggatct
      841 ggtggttcag catacaagct gcgttcaaac ggaagatagg gggcagcaat attactctcc
      901 attggtctgc ctatgccatc aacaagccgc ccaagtagtg cgtctccaac acgaatcttg
      961 aactcatctc cctgataact taaccactga ccacagtaca tgccggaaac atgctcaaaa
     1021 ggcaacaaga aaacctcatc ttcatcaata gcgatcacct cggctaaacg ttgactgggt
     1081 tctatcttat aaaaggcgcc aatacgcgct tttggtaaac gcgccttaat aatcgtcccg
     1141 ccgatattga taatcttgcc ttcatatctg gtagacgaat ttaatgataa tgccggcaca
     1201 gtgctattga gcactttctg aatacgtggg tatttttcca atacagaatc atgctctgaa
     1261 atcatttacc gttcctaata ctttaagttc gatattattc ccaacctcct gaaaagagag
     1321 cacggggact gacgggaacg aaccttcaat cattttcttc acatagcgcc tgatatctaa
     1381 agcggtcaga atgacgccgt tccccgttgg attcatgata ttttttaaga agccaattat
     1441 ctgctcagtt cgctccggtg agatattcag atacgaacct gatgacgttt gtctgatcga
     1501 ctctcgtagc tcattttcaa tatcagagcc aataagccaa acgttcagaa gtgtaccgct
     1561 aacgctatag cgacctaaaa tatgccgacg cagggcaata cgaacgtatt cgcacaagat
     1621 aaccacatcc ttttcttttg ttgaccaaaa aataagcgtc tcaaagatag ttctcaggtc
     1681 tctaattgag acattttcct ctacgagacg ttgtaggatg tcaacaattt tgctcaaacc
     1741 aagctggcgc tgcagctctt tcacaagctc gttatatttt ctctccatga tgtccatcaa
     1801 ataacgcgtt tcttgtacgc cgatgaactc tttggcatta gacaatacaa gtttctttag
     1861 caaaaacgtt atcttatcat ttgttgttgc ataaacatcc atacctgtaa ggtgagcgag
     1921 ctgttgatta gtcttattcg tcttatatac gatagagtta gtcgaaagct tttcctggac
     1981 aaattcgaat gatatctctg catgcccagc ctcaaaatag acagtatcat ctataagagt
     2041 atctttgtat attgactctt gatagagcaa aaattctatt gcgctatcat tatttttaac
     2101 cggattaaca ataatcttag gcaatggaat tccactttcc tcaaagaaat tccaacgcat
     2161 gacctcaatt tgctgtgtaa tgtctgaact ataaatatta ctacttaagt ttaaaatcaa
     2221 tggctcagcg cccggtgaga tgttttggct attagacaaa tcagctccca tagcatcctt
     2281 attattgcca gttccaaatg ttcttttctt ctgtaatttc caggctatcc ccaacaggca
     2341 aaccgccaga aataagaaga ccaatgcagg aaagcccgga ataatcgcca ttacgagcat
     2401 tagtgcggca ctgattaaaa aagggcgact attaacagaa acctgttgaa caatatcacc
     2461 tgcaaggttt tcttcttttt cgctttcacc gggcacacga gtaacaacca cgcctgcggt
     2521 gacagaaata ataagcgcag ggatctgggc aaccaaagca tcaccgacag acaatacaga
     2581 aaacaggcta agtgccgcac taaatggcat gtcaaattgc cacataccaa tgagcacgcc
     2641 accaaaaagg ttaactaaaa caataatgat accagcaata gcgtcccctt ttacaaactt
     2701 catcgccccg tccattgaac cataaaactg gctttccttt tgcactcggg agcgaagaac
     2761 ttttgcctca agcgggtcaa taacgcctgc gcgcatatca ccatcaatac tcatttgctt
     2821 tcctggcata ccatcaaggg aaaaacgagc actcacttca gcaacacgct cggccccttt
     2881 tgtaataacc ataaattgaa caatggtgat aatagtaaaa ataaccaggc caacaacaat
     2941 attaccgcca acaacaaagt taccgaagga gtaaataatg tcacccgcat cgtgatgcag
     3001 cagaataagt ctggttgtac taacactgag cgacaggcgc atcaacgtcg taatcaacag
     3061 gattgtgggg aaagaagtta gttcaagagg attttttata tagattgaga gcattaataa
     3121 aagtaaagct gtcgaaatat taatcgcgat aatgatatcc accacaactg ttggtagtgg
     3181 aataatcatc atcattacag ccataaagaa gaagagagcc agaataagat cgtgatatga
     3241 ctctgctttt ttaaatatat ttaagagttt attcatgatg tcatcctgcg aacgcgctca
     3301 ataatctgca catgctgttg aaaagtattc attagaaaaa aaacatcgat attggttaac
     3361 aaacaactta agcacaattt attatcgtag acaaaaatat gttcaatcac tccctgcgta
     3421 cgctcaggag caagggcact aaagaaagag aaaaatgcgt tttcgttata ctctaaagca
     3481 aacgtaacaa aaacattatt atcaattatt tcaataaaaa aagaaaaccc attaaaatca
     3541 atttcaacac ggt
//
LOCUS       DBFIFS010000310         3542 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.311, whole genome shotgun sequence.
ACCESSION   DBFIFS010000310 DBFIFS010000000
VERSION     DBFIFS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.311"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            280..1212
                     /gene="rfaD"
                     /locus_tag="N7A07_004578"
     CDS             280..1212
                     /gene="rfaD"
                     /locus_tag="N7A07_004578"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU2479089.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1222..2268
                     /gene="rfaF"
                     /locus_tag="N7A07_004579"
     CDS             1222..2268
                     /gene="rfaF"
                     /locus_tag="N7A07_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU2479090.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2272..3264
                     /gene="rfaC"
                     /locus_tag="N7A07_004580"
     CDS             2272..3264
                     /gene="rfaC"
                     /locus_tag="N7A07_004580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU2479091.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3261..>3542
                     /locus_tag="N7A07_004581"
     CDS             3261..>3542
                     /locus_tag="N7A07_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU2479092.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASM"
BASE COUNT          910 a          853 c          898 g          881 t
ORIGIN      
        1 aaaagaatta tatcataaat attaacagaa tgtatactgt atattatcca ttcaaatgct
       61 tccgttattt atcatacatc ggaatattga tactaaagca ctattcacat gcaaaaccaa
      121 catccgccat gaaggactag ctaaaaccca aactagtttg ttgcaattag catccttgca
      181 cctctatgta aagggctgaa gggattcgga tgtgatggta tgattacaga cattcgtgtc
      241 tgagattgtc tctgactcca taattcgaag gttacagtta tgatcatcgt taccggcggc
      301 gcgggcttta tcggcagcaa catcgttaaa gccctgaatg ataaaggcat caccgatatt
      361 ctggtggtgg acaacctgaa agacggcacc aagtttgtga acctggtgga tctgaatatc
      421 gcagactata tggataagga agacttcctg atccagatta tggctggcga agagttcggc
      481 gatgtcgaag cgattttcca cgaaggcgcg tgctcttcca ccaccgagtg ggacggcaag
      541 tatatgatgg ataacaacta tcaatactcc aaggagctgc tgcactactg cctggagcgt
      601 gaaatcccgt tcctgtacgc ttcttccgca gccacctacg gcggacgcac ctccgacttt
      661 attgaatccc gcgagtacga aaaaccgttg aacgtctacg gttactcaaa attcctgttt
      721 gatgaatatg ttcgccaaat cctgccggaa gcgaactcgc agattgttgg cttccgctat
      781 ttcaacgttt atggaccgcg tgaaggccat aaaggcagca tggcgagcgt cgctttccat
      841 ctcaacaccc agcttaataa cggtgaatca ccgaagctgt tcgaaggtag cgagaacttt
      901 aaacgcgact tcgtctatgt tggcgatgtg gcagatgtaa acctgtggtt cctggaaaat
      961 ggcgtttccg gcatcttcaa cctcggcacc ggtcgtgcgg aatccttcca ggctgtagct
     1021 gatgctacgc tggcttatca caagaaaggc cagatcgaat acattccgtt cccggataag
     1081 ctgaaaggcc gctaccaggc gttcactcag gcagatctga caaatctgcg cgcggcgggt
     1141 tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg aatacatggc ctggctgaat
     1201 cgcgacgcat aagagctctg catgaaaata ctggtgatcg gcccgtcttg ggttggcgac
     1261 atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc gctatcccca ggcgataatc
     1321 gatgtgatgg caccggcatg gtgccgtcca ttattatcgc ggatgccgga agttaacgaa
     1381 gctattccta tgcctctcgg tcacggagcg ctggaaatcg gcgaacgccg caaactgggt
     1441 catagtctgc gtgaaaagcg ctacgaccgc gcctacgtct tacctaactc cttcaaatcc
     1501 gcattagtgc ctttcttcgc gggtattcct catcgcaccg gctggcgcgg cgagatgcgc
     1561 tacggtttac tcaacgatgt acgcgtgctc gataaagaag cctggccgct aatggtggaa
     1621 cgctatgtcg cgctggccta tgacaaaggc attatgcgca cagcacaaga tctgccgcag
     1681 ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa aatcatatac ctgtaatcaa
     1741 ttttcgcttt catcagaacg tccgatgatt ggtttttgcc cgggtgcgga gtttggtccg
     1801 gcaaaacgct ggccacacta ccactatgcg gagctggcaa agcagctgat tgatgaaggt
     1861 tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag caggcaatga gattcttgcc
     1921 gctttgaata ccgagcagca ggcatggtgt cggaacctgg cgggggaaac acagcttgat
     1981 caagcggtta tcctgattgc agcctgtaaa gccattgtca ctaacgattc tggcctgatg
     2041 cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt atggtccgag tagcccggac
     2101 ttcacaccgc cgctatccca taaagcgcgc gtgatccgcc tgattaccgg ctatcacaaa
     2161 gtgcgtaaag gtgacgctgc ggagggttat caccagagct tgatcgacat tactccccag
     2221 cgcgtactga aagaactcaa cgcgctattg ttacaagagg aagcctgacg gatgcgggtt
     2281 ttgatcgtta aaacatcgtc gatgggcgat gttctccata cgttgcccgc actcactgat
     2341 gcccagcagg caatcccaga aattaagttt gactgggtgg tggaagaagg gttcgcacag
     2401 attccttcct ggcacgctgc cgttgagcga gttattcctg tggcaatacg tcgctggcgt
     2461 aaagcctggt tctcggcccc tataaaagct gaacgcaaag cgtttcgtga agcgctacaa
     2521 gcagagaact atgacgcagt tatcgacgct caggggctgg taaaaagcgc ggcgctggtt
     2581 acgcgtctgg cacatggcgt aaagcatggc atggactggc aaaccgctcg cgaaccttta
     2641 gccagcctgt tttacaatcg taagcatcat attgcaaaac agcagcacgc cgtagaacgc
     2701 acccgcgaac tgtttgccaa aagtttgggc tatagcaaac cgcaaaccca gggcgattat
     2761 gctatcgcac agcattttct gacgaacctg cctacagatg ctggcgaata tgccgtattt
     2821 cttcatgcga cgacccgtga tgataaacac tggccggaag aacactggcg agaattgatt
     2881 ggtttactgg ctgattcagg aatacggatt aaacttccgt ggggcgcgcc gcatgaggaa
     2941 gaacgggcga aacgactggc ggaaggattt gcttatgttg aagtattgcc gaagatgagt
     3001 ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg tagtgtcggt ggatacgggg
     3061 ttaagccatt taacggcggc actggataga cccaatatca cggtttatgg accaaccgat
     3121 ccgggattaa ttggtgggta tggggagaat caagtagagt gtcgttctac aagtatgtct
     3181 cttgcagatt tgccagctca aaccgttttt caaaatttaa atcttgaaat aataaccaat
     3241 aagttgacat cggagataag atgacctcaa cattattttt ctctctcgag aaaaaaaact
     3301 ggatagcgta ctggaacaga gctctcgtat tcttattcat taccacctat tttttgggtg
     3361 ggattacaag gtataaacat cttattgtta ttcttatgac aataacgaca atcgtctatc
     3421 tctgcaaacg gccaaaacac tatctctcac tatttaaaac atttcttttt ggtagtgttg
     3481 ccatattaac tattgctgca ttgctgtcac ttcttcaatc ccctgatgca ggtgctagca
     3541 tg
//
LOCUS       DBFIFS010000311         3528 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.312, whole genome shotgun sequence.
ACCESSION   DBFIFS010000311 DBFIFS010000000
VERSION     DBFIFS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.312"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            68..804
                     /locus_tag="N7A07_004582"
                     /pseudo
     CDS             68..804
                     /locus_tag="N7A07_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            983..1279
                     /locus_tag="N7A07_004583"
     CDS             983..1279
                     /locus_tag="N7A07_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2479093.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1587..1703
                     /locus_tag="N7A07_004584"
     CDS             1587..1703
                     /locus_tag="N7A07_004584"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479094.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1922..3307
                     /locus_tag="N7A07_004585"
     CDS             1922..3307
                     /locus_tag="N7A07_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU2479095.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
BASE COUNT         1074 a          690 c          791 g          973 t
ORIGIN      
        1 cagaaggccc cggtgatgcc ttctgacgat agattaatgt tgtattctat tggtatctgg
       61 agaccatatg gcaacagtta cagtatattc tccccgctgt aattcctata aagtgtaccg
      121 ttatgatcac agcacctcag atcatgagcg tttccagtgc taatcctgca aacgtgtatt
      181 tcaactgaat tacatctacg aaacccgaaa accaggcatt aaagagcaaa ttgttgaaat
      241 ggttcataac ggagccgggg ttcgcgatag ctccagaacg ctgaaggtgg atatcaacac
      301 cgtcattctc actttaaaaa acgctcacca cgtaaagtaa ccatatctcc agccgccagt
      361 gtcgatgtgg cacttatctg tgaacttgat gaacaatgga gttttgtcga aaacaaagct
      421 cgtcagcagt ggcactggta cgcgtataag accaaagctg acggtgtgct ggcttacact
      481 tttggtcctc gcactgatga aacatgccgt gagttgccgg aattcctgaa gccattctcc
      541 gcaggcatga taaccagaga caaccggagc agttatacca gagaaatgcc acaggataag
      601 catcttgtcg gcaagatatt tacccggcgc attgagcgca acaatctgac acttcgtact
      661 cacatcaaac gacctgcccg gaaaacgatt tgctttttac gctccctgga aattcacgaa
      721 aagccattgg ttcatttatc gaaaacacat gtactactga caggagtcat taccagggcc
      781 tcttttgctg tttttttgcc atgaatgata atgagaatcg acagcgataa gggaaaagta
      841 aaataacact aatgctgtcg ttttctatca tattgatttt taattattta ttgtcttttt
      901 tctcaaccca tttttttacg cagacaaatt tacccgggga atacaaactg taacgacagt
      961 tcaccacact atgggggata aaatgccaga tcaatccgga cactgcagga ctccttcacg
     1021 catcttacgg atgcctgaac tgagccaatt gttaggtata tctcgttcga caatttacga
     1081 aaaaatgaat ccgttatcga aatactacga tgcaaccttc cccaggccgg tacgtcttgg
     1141 tagtggttct gtgggctggc gttcatcagc tattgacgaa tggcttactt tacatactgt
     1201 acctgccaga tcagcagtca aaggtgttaa tgacgaagtg agacatacgc tttcttcaga
     1261 tatgaccatg agcagataag tttttgtcat cacgtgatca cctcaggagg aaaaagaagc
     1321 ataagaacaa tttagccaga agtttaattg ataaacagaa tttccagcat tcattgttga
     1381 aagagcagct attgtcgcta ttgtagcggt aattgctgct ataaccgaaa cgtattacga
     1441 aaaaggaagc atttatgcct tacatcaaac cggatatgtc gtattcagta tatgcatgac
     1501 catcaatgat agggaaggca ataattaaag ctcaatacaa taaacaagta cctctgactc
     1561 tggttgcaat gtcagtatta acagcgatgt caattgcctg tcagaatcag gttgacgtat
     1621 gcagtcccgg aaatttacgt ggggccgtta atatttacac tatggttctg gagaaaggga
     1681 aaagaccggg ggaaaggtat tagtgaaata atttccggag gtattaagaa tgagaaatat
     1741 gaatgatatt tcgtggtgaa taatacagtc ttcactgaat taaccttatc ggtttattaa
     1801 acatcagatc tacaaatgct ctgtatttct cagagcatca ggtctgaata gcagctattt
     1861 tcgctattgt agcgggattt gctgctattt tactaaccca ttaatgaaaa gagaggcttt
     1921 tatgtgttta cttgcaccag aaaatcctta tccgatatat gcactaccac cgctggtgag
     1981 aaatgcaata attgaaactc aaaaaaatac acaggcacct ttggctatgg tggcaacatc
     2041 cgcattaaca gcgatctcaa ttgcctgtca gaatcagatt gacgtgtgca gacctggaaa
     2101 tttacatggg cctgttaatc tttactctct gattctggct gattccggtg aaaggaaaac
     2161 gaccgtggat aaagtgttta tgaaagcatt ttatctcagg gatgaagccc tggcggatga
     2221 atacgcgaaa ctggttgaga attacagtac agaaaaggaa atatgggagc aaaaacaaaa
     2281 agcgctggaa tcaaaatttc ataaagagat tcgtgccggt aaagattaca aggcaacaga
     2341 atcagagctt gaaacgcatc tgaataaacc tcctgttccg ccgcagatac gtcgaacaat
     2401 ttttaatgag acaacgatag agggaatgtt aaaatattac tccgatagca atcgctcttt
     2461 tgctcttgta tccagtgaag ggggggtaat ttttgacagc cgggccatga gtaaactggg
     2521 aattattaac actttgtggg atggaggttc tcttttcatc gacaggaaat catctcccgg
     2581 aattaatttg aaggagccaa gactgacgat atcggtgatg attcagcctg atgtttacca
     2641 caaaggtttt tgtacgcgaa aaaaagaaat tgtgaaaacg tcaggacatc atgcaaggtt
     2701 tttgatgtgt caaccaacat caacgcaggg gacaaggata atgatcaatg agcttattga
     2761 tgaaagtctg gcgatgagtg gtgaacgacg ttgccttcac ttttctcctc aggctgccag
     2821 aatctggacg gattattaca atgatgtgga atcgaagctg gggggattgg ggcctttaag
     2881 acattgccgg gaatatgctg ctaaaaatgc agagtatatg gcaagactgg caggacttat
     2941 ttaccattcg agcggtgaag agggggagat ttccccttac actgcagaaa tggcgagaga
     3001 attagcaata tggtacggta atgagtatgt gcggttgtct aatccgttaa cttttgacaa
     3061 ctctgccctg accgtacctg tgcgactcat tccagaggag cttgaacttt tcaactggat
     3121 aaaaagctat tgtattgaga aggggatcct ctgtatgaaa aaaaatgaca ttttacagcg
     3181 tggtccgaac cgtttccgga aaaaggataa aatcaactgg ttactggatt tactgtatga
     3241 acaaaacaga gttgtaccgg ttattgaggg aaaaacgttg tgtgtcgcac ctaactttga
     3301 cctctgaacg acttaatcgt tgagtacata tagtctcctc tgctacttgc ggaggagact
     3361 aatgtcttta ccatgtcagc gttgtattag aaaactctgg tacaggtcag tcagtaaata
     3421 taactggtgt aaccactatt tactattgga tagagtgttg ctcaggatat tgtcggcttt
     3481 agtctgaggt gaggtggggc cttcattgtt ttcgtgttca ataaataa
//
LOCUS       DBFIFS010000312         3494 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.313, whole genome shotgun sequence.
ACCESSION   DBFIFS010000312 DBFIFS010000000
VERSION     DBFIFS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.313"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            31..390
                     /locus_tag="N7A07_004586"
     CDS             31..390
                     /locus_tag="N7A07_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2479096.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="N7A07_004587"
     CDS             399..929
                     /locus_tag="N7A07_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU2479097.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="N7A07_004588"
     CDS             1171..1368
                     /locus_tag="N7A07_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU2479098.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="N7A07_004589"
     CDS             1519..2577
                     /locus_tag="N7A07_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU2479099.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="N7A07_004590"
     tRNA            2618..2693
                     /locus_tag="N7A07_004590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="N7A07_004591"
     tRNA            2774..2850
                     /locus_tag="N7A07_004591"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
     gene            3374..3466
                     /locus_tag="N7A07_004592"
                     /pseudo
     CDS             3374..3466
                     /locus_tag="N7A07_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          898 a          763 c          993 g          840 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aaggggagga ataatgacat ttaaacatta tgatgttgtc agggcggcgt cgccgtcaga
     3421 ccttgcggaa aagctgacac acaaactgaa agagggctgg caaccgctat tctgaacgca
     3481 gccgggcgca cctc
//
LOCUS       DBFIFS010000313         3484 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.314, whole genome shotgun sequence.
ACCESSION   DBFIFS010000313 DBFIFS010000000
VERSION     DBFIFS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.314"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..933
                     /locus_tag="N7A07_004593"
     CDS             <1..933
                     /locus_tag="N7A07_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2479100.1"
                     /translation="RLFCEGGVRPPLEKIMPLLDKLREQYGVGPVCSELHIAPSTYYH
                     CQQQRHHPDKRSARAQRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVA
                     RLMAVMGLAGVLRGKKVRTTVSRKAVSAGDRVNRQFVAERPDQLWVADFTYVSTWQGF
                     VYVAFIIDVFAGCIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYISLA
                     YTERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLGRLGHIPPAEAEKAYYASIRNDDLAA"
     misc_feature    1..117
                     /locus_tag="N7A07_004593"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1527..1718
                     /locus_tag="N7A07_004594"
     CDS             1527..1718
                     /locus_tag="N7A07_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2479101.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(1771..2004)
                     /locus_tag="N7A07_004595"
     CDS             complement(1771..2004)
                     /locus_tag="N7A07_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479102.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(2100..2723)
                     /locus_tag="N7A07_004596"
     CDS             complement(2100..2723)
                     /locus_tag="N7A07_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2479103.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(2812..3321)
                     /locus_tag="N7A07_004597"
     CDS             complement(2812..3321)
                     /locus_tag="N7A07_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479104.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          975 a          874 c          817 g          818 t
ORIGIN      
        1 cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaataatgcc gctgctggat
       61 aagctgcgtg agcagtacgg ggtcggaccg gtatgcagtg aactgcatat tgccccgtca
      121 acgtattacc actgtcagca acagcgacat catcctgata aacgcagtgc ccgtgctcag
      181 cgcgatgact ggctgaagag agagatacag cgcgtatacg atgaaaatca tcaggtgtac
      241 ggtgtgcgta aagtctggcg ccagttgtta cgcgaaggta tcagggtggc cagatgtaca
      301 gtggcgcgcc tcatggcggt tatgggactt gccggtgttc tccggggtaa aaaggtccgt
      361 actaccgtca gccggaaagc cgtttccgca ggcgaccgcg taaaccgtca gttcgtggca
      421 gaacgtcctg accagctgtg ggtggctgat tttacttacg tcagcacatg gcagggcttc
      481 gtctatgtgg cgttcatcat tgatgtgttt gccggatgta tcgtggggtg gcgagtctca
      541 tcgtctatgg aaacgacatt cgtgctggat gcactggagc aggcgttgtg ggcccgtcgg
      601 ccgtccggca cagtccatca cagtgataaa ggttctcagt atatatcact ggcctatacg
      661 gagcgactaa aagaagcaaa actgctggca tcaacaggga gtacaggtga ctcgtatgac
      721 aacgcgatgg cggagagcat caatggtctt tacaaagcgg aggtaataca ccgtaagagc
      781 tggaaaaacc gtgcagaagt ggaactggcc acactcacgt gggtggactg gtataacaat
      841 cgacgattgc tgggaaggct gggtcatatc cctccggcag aagcagaaaa agcttattat
      901 gcttccatca gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
      961 aaccggggcg gttcaccagc ctcctgacga tacctcccac catgttttgc gatggcacgc
     1021 taccgccctt ccggtattta cccgcatcaa ccggtaccag aatgctgagt aacaaaccag
     1081 ctagaattga cttaattttg atcattatag aataatgatc tgaactacag caacatgaca
     1141 gatttatcga tcgatataac acaaatgaac agttctttat tctgacaaat atcgcaaaaa
     1201 cagcacatta caaaatttat aaccataatt actggaaaag tgcagaaaat tattccactg
     1261 aatgctttta atacagttgg ttagcttaaa aaatagcaat ttttgatttc agtcattgaa
     1321 agttgcaatg tttgacgatt caacagcatt taagtgagtt tcaggatatg ggaaggacat
     1381 gaaaggagga gcgcaataaa atacacagga agaaggggaa aatgtttcgt aaaaacagaa
     1441 aattaacagc gcacatcagg aaacgcagtc tcctgattca gtgctaccac atgaaaattt
     1501 agtcaactgt taccgtacta aaatttatgt cagaagatgt tccattaccg aaagtcaatc
     1561 agcgatacaa agacgatcat ggtgcgcttg tcaccctgac cagtgttgag gaaacccggg
     1621 tggtgtttat gcaggacggc tatccgcatc cctgcatgcg accgatgtac aactttctgg
     1681 gaaaattcaa acccgaaccc cgggaagaaa cagaataaaa cgaaatccct gccggcaatc
     1741 acactacctg atagcagcat aatccactta ttaattctca tccccggaga caagcccaga
     1801 aaaccactcc gggtgttcac tgtatgccgt gttcagcgca tccctggaaa cgtttatata
     1861 aaagctgtcc tgcaatttgc gggtaacaac ttctgccagg cgattccggt tatccaccag
     1921 tccggcataa cctctctctg ccagccactc attcagttca tgttccagag acagattgat
     1981 aatatcataa tcggaccttg ccattcccgt ttccttagca catgttttca ggcacgggga
     2041 taaagtataa agaatgtcat actgctgctg ccagagcaat attccccctg atggttgtat
     2101 cagagatggt atgccggtac tttgcgggtt gttcccgtca ggttatcgtg cacctccacg
     2161 gtcgtggtca ccaccggcat tccggccttt ctcagcctca gaacatcagc cgcaatacgc
     2221 tgactgccaa acctgaacag gccgtccagt gcagtcacca gtaaacccgc ctccagtgca
     2281 tacttcagcc gttcacgggg cgctttcact tcccgggcaa cctgctgata tggcaatgat
     2341 gtgttttcat tcccaaccac gcgacgaata cggtgagaca gatgataccg gtatgtatcc
     2401 ggcacaccag aaaggctggc cttcaggctg tacacgcagc caaaccgttt atcgttaaac
     2461 accacatttt tctggctgat gccccattct tcacgcagcg cggcaatcaa ttgcggtgta
     2521 cgggtaagca gcaagcgaaa aggcagttca aaactggtga cgtaatccac attcagcagt
     2581 gcaatacgca accgttcttc tggcccggcc tctgtctgcc ggcactcctc cagaacatcc
     2641 tgccactgcc ggcgaagacg gggggattcg ttccgttctg taaaacagta tttatccgcc
     2701 agatagtcaa ttcgtgtacg catactgaaa ggaattccgt ataaagattc agcagacaaa
     2761 actttatcag ccagtaaaaa ttaacggaag cattgatatt cctcccgaca atcaccggat
     2821 gattgttgca ataacctgcg acataaagga ctgaacagcc gtttttaacg ccacgtattg
     2881 ccctgatgaa ccaggccggg ccacccgaaa acatctttcc acccggcatt gtccgccagc
     2941 acttatcacc gaccaggtct gttgcagtaa atccaccagt tgcgcacatt cttcatcagc
     3001 catgacgccg actcgcggat ggcaaatact cgtctggctg agcagcaaca gacatcgtat
     3061 ctgtgctcct tcatgttgtt cccgcaacca gcgtaatacc accggcgctg atgggctgac
     3121 cgtgtgatta tgctccgtaa tacgttcgtg tacccgtcgg tgaaaggaac tacaaaatgt
     3181 ctgaatctgt tgaccataga ttccttctgc cgaatagagc gtgaagtgaa atgccaacag
     3241 atttggccag tggtccaccg cctgctgaac aaaacgccgg atttcccccg gctctgaaat
     3301 taaggcttcg gttatttgca cgaaatgttc cttttcaacc cctgttgaat tttgtcaagc
     3361 cggtgcagac acagtaaagc gcgcatcaac acctgaaagg tacgatacaa acacaagtta
     3421 caaacaataa gaacggaagg acaattaatc agtgaggaat aataactaaa ttaaccaaaa
     3481 gaat
//
LOCUS       DBFIFS010000314         3472 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.315, whole genome shotgun sequence.
ACCESSION   DBFIFS010000314 DBFIFS010000000
VERSION     DBFIFS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.315"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..194
                     /locus_tag="N7A07_004598"
     CDS             <1..194
                     /locus_tag="N7A07_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HGU2479105.1"
                     /translation="KITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCF
                     RSNTLGAKMQALRIFLSRK"
     gene            469..1665
                     /gene="kbl"
                     /locus_tag="N7A07_004599"
     CDS             469..1665
                     /gene="kbl"
                     /locus_tag="N7A07_004599"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU2479106.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1675..2700
                     /gene="tdh"
                     /locus_tag="N7A07_004600"
     CDS             1675..2700
                     /gene="tdh"
                     /locus_tag="N7A07_004600"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU2479107.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            2939..>3472
                     /gene="waaH"
                     /locus_tag="N7A07_004601"
     CDS             2939..>3472
                     /gene="waaH"
                     /locus_tag="N7A07_004601"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU2479108.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVK"
BASE COUNT          827 a          788 c          938 g          919 t
ORIGIN      
        1 aaaaaataac actaaataat aacattgtgg atgatgacca gggcattttt gtcatgtgct
       61 attacaaacg ccccgatctc ttcaacctaa actatctggg gagaggaaaa tggttcgatt
      121 tgtttcgctg cttcaggagt aacactttag gggcaaaaat gcaggcgctg agaattttcc
      181 tttcaagaaa ataggtttcc ctccattagt gggtagcgtt ttgataccaa ctgagatagg
      241 aaaaatgatg ctttggcatc attttttctt tttatatcaa tgcagtaaaa tcaatcatca
      301 ttatgcccag ccaaaactgt acagtttttc tactatctgt aggtgttgag agacatcaag
      361 ttacacgtca tttatcctga attttgcaga agagttaacg cgtcatctcg tcgcgaccta
      421 taagtttggg taatatgtgc tggaatttgc cctgtctgga gaatcgcaat gcgtggagaa
      481 ttttatcagc agttaaccaa cgatctggaa accgcacggg cggaagggtt gtttaaagaa
      541 gagcgcatta ttacgtctgc gcagcaagca gatatcactg tggctgatgg aagccacgtc
      601 attaactttt gtgccaacaa ctatctcggg ctggcgaatc atccggatct gattgcggca
      661 gcaaaggcgg gaatggattc tcacggtttc ggcatggctt cggtgcgttt tatttgcggc
      721 actcaggaca gccataaaga gcttgaacaa aaactggcgg cattcctggg gatggaagat
      781 gcgattctct actcttcctg ctttgatgct aacggtggac tgtttgaaac gctgctgggt
      841 gcggaagacg ccattatctc cgacgcactg aaccacgcgt ctattattga tggtgtgcgt
      901 ctgtgcaaag ctaaacgcta tcgctatgcc aacaacgata tgcaggagct ggaagcacgt
      961 ctgaaagaag cgcgtgaagc cggtgcgcgt catgtgctga ttgccaccga tggtgtgttc
     1021 tcaatggacg gcgtgattgc caacctgaaa ggcgtttgcg atctggcaga taaatacgat
     1081 gccctggtga tggtggatga ctcccacgcg gtcggttttg tcggtgaaaa tggtcgtggt
     1141 tcccatgaat actgcgatgt gatgggccgg gtcgatatta tcaccggtac gcttggtaaa
     1201 gcgctgggcg gggcttctgg tggttatacc gcggcgcgca aagaggtggt tgagtggctg
     1261 cgccagcgtt ctcgtccgta cctgttctcc aactcgctgg caccggccat tgttgccgcg
     1321 tccatcaaag tactggagat ggtcgaagcg ggcagcgaac tgcgtgaccg tctgtgggcg
     1381 aacgcgcgcc agttccgtga gcaaatgtcg gcggcgggct ttaccctggc gggagccgat
     1441 catgccatta ttccggtcat gcttggcgat gcagtagtgg cgcagaaatt tgcccgtgag
     1501 ctgcaaaaag agggcattta cgtcaccggt ttcttctatc cggtcgttcc gaaaggtcag
     1561 gcgcgtattc gtacccagat gtctgcggcg catacccctg agcaaattac gcgtgcagta
     1621 gaagcattca cgcgtattgg taaacaactg ggcgttatcg cctgaggatg tgagatgaaa
     1681 gcgttatcca aactgaaagc ggaagagggc atctggatga ccgacgttcc tgtaccggaa
     1741 ctcgggcata acgatctgct gattaaaatc cgtaaaacag ccatctgcgg gactgacgtt
     1801 cacatctata actgggatga gtggtcgcaa aaaaccatcc cggtgccgat ggtcgtaggc
     1861 catgaatatg tcggtgaagt ggtaggtatt ggtcaggaag tgaaaggctt caaaatcggc
     1921 gatcgcgttt ctggcgaagg ccatatcacc tgcggtcact gtcgcaactg tcgtggcggt
     1981 cgtacccatc tgtgccgcaa caccattggt gtcggtgtta accgtccggg ctgcttcgcc
     2041 gaatatctgg tgattccggc gttcaacgct ttcaaaatcc ccgacaatat ctccgacgac
     2101 ctggcttcca tttttgatcc cttcggtaac gccgtgcata ccgcgctgtc gttcgatctg
     2161 gtgggcgaag atgtgctggt ttctggtgca ggtccgatag gtattatggc tgcggcggtg
     2221 gcgaaacacg ttggtgcacg caatgtggtg atcaccgatg ttaacgaata ccgccttgag
     2281 ctggcgcgca aaatgggtat cacccgtgcg gttaacgtcg cgaaagaaaa ccttaatgat
     2341 gtgatggctg aactgggcat gaccgaaggc tttgatgtcg gtctggaaat gtccggcgcg
     2401 ccgccagcgt ttcgtaccat gctcgatacc atgaaccacg gcggtcgtat tgcgatgctg
     2461 ggtattccac cgtctgacat gtctatcgac tggaccaaag tgatctttaa aggcttgttc
     2521 attaaaggca tttacggtcg tgagatgttt gaaacctggt acaagatggc ggcgctgatt
     2581 cagtctggcc tcgatctctc gccgatcatt acccatcgtt tctctatcga tgatttccag
     2641 aagggctttg acgctatgtg ttcgggccag tccgggaaag ttattctgag ctgggattaa
     2701 cacgaaaaag ggctggtatt ccagcccttt tgcctgagga taatctgtta aatatgtaaa
     2761 atcctgtcag tgtaataaag agttcgtaat tgcgctgatc ttttatatag ctgttctcat
     2821 tatctctcta ccctgaagtg actctctcac ctgtaaaaat aatatctcac aggcttaata
     2881 gtttcttaat acaaagcctg taaaacgtca ggataacttc agaggtcgtc ggtaatttat
     2941 gatgaacagc accaataaac ttagtgttat tattccgtta tataatgcgg gcgatgattt
     3001 ccgcacttgt atggaatctt taattacgca aacctggact gctctggaaa tcattattat
     3061 taacgatggt tcaacggata attctgttga aatagcaaag cattacgcag aaaactatcc
     3121 gcacgttcgt ttgttgcatc aggcgaatgc tggcgcatcg gtagcgcgta atcgtgggat
     3181 cgaggtggcg acgggcaaat atgtcgcttt tgtcgatgct gacgatgagg tctatcccac
     3241 catgtacgaa acgctgatga ccatggcgtt agaggacgac ctcgacgtgg cgcagtgcaa
     3301 cgctgactgg tcttttcgtg aaacgggaga aacctggcaa tccatcccca gcgatcgcct
     3361 tcgctcaacc ggtgtattaa ccggcccgga ctggctgcgg atggggcttt cttcgcgccg
     3421 ttggactcac gttgtctgga tgggggttta tcgccgtgat gttattgtta aa
//
LOCUS       DBFIFS010000315         3455 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.316, whole genome shotgun sequence.
ACCESSION   DBFIFS010000315 DBFIFS010000000
VERSION     DBFIFS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.316"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            177..3105
                     /gene="ehaB"
                     /locus_tag="N7A07_004602"
                     /pseudo
     CDS             177..3105
                     /gene="ehaB"
                     /locus_tag="N7A07_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
     gene            3193..>3455
                     /locus_tag="N7A07_004603"
     CDS             3193..>3455
                     /locus_tag="N7A07_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2479109.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLIS"
BASE COUNT          962 a          771 c          788 g          934 t
ORIGIN      
        1 acttattctt tgtgcgtata gatattttta tatatgtccg caatcggact taggggaaag
       61 aataattgaa ccattcctct ataacgccgc ataatcgtca acgcgaaaca taagatgttt
      121 tctatgcatt gataattgat ggatcaactt attacgtccc tgaggaggga tgacaaatgc
      181 actcctggaa aaagaaactt atagtatcac aattagcatt ggcttgcact ctggctatca
      241 cctctcaggc taatgcagcg accaacgata tttctggtca aacttacaat actttccatc
      301 actacaacga cgccacctat gctgatgacg tttactatga tggttatgta ggctggaaca
      361 actatgccgc tgatagctat tacaacggcg atatctaccc ggtcattaat aacgctaccg
      421 ttaacggcgt gatttctacc tactatctgg acgacggtat ttctaccaat accaacgcca
      481 atagtctgac aatcaaaaac agcactattc acggtatgat tacctctgag tgcatgacta
      541 ctgattgtgc tgatgaccgt gctactggtt atgtttatga tcgtctgaca ctgagcgttg
      601 ataattcaac gatcgatgac aactacgagc attatactta caacggtacc tataataatg
      661 ccgctgacac tcatgttgta gatgtttacg atatgggtac tgctattaca ctggatcagg
      721 aagttgatct gtccatcact aataactctc atgtagcagg tattacgctg actcagggtt
      781 atgagtggga agatattgac gacaacacag tcagcactgg cgtaaacagc agcgaagtgt
      841 ttaataacac tattactgtt aaagattcta ctgtgacctc tggttcatgg actgatgaag
      901 gtactactgg ttggtttggc catactggta atgccagcaa ctatagcaac acgctgactg
      961 cagacgatgt tgcaattgcc gcaatcgcaa atccgtatgc tgataatgcg atgcagacta
     1021 cagtaacttt agacaactca acactgatgg gtgatgttgt tttctccagt aatttcgatg
     1081 aaaacttctt cccacaaggt gctaacagct atcgcgatgc tgatggtgat gtagatacca
     1141 acggttggga tggcacagac cgtatggatg tgactctgaa caacggcagc aagtgggttg
     1201 gcgctgcaat gtctgttcat atggttgatg aagatggtga tggttcttac gacggatatg
     1261 ctgttggtac tgaagcaact gcaactctgc tcgatattgc agctaacagc ctgtggcctt
     1321 catcaactgt cggtgttgat aacatcaata ctcaatatga cgaaaatggc catatcgtag
     1381 gaaacgaagt ttaccagagc ggtttgttta atgtgacttt gaacggtggt tcagagtggg
     1441 atacaacaaa atcttctctg attgatactt taagtattaa cagcggttcc caagttaatg
     1501 ttgcagactc tcgtctgatc tctgactggc ggttctaacc tgaacatcgg tgaagacggt
     1561 catgtagcga ctaataccct gaccatcgac aatagtaccg ttaaaatgtc tgatgatgtt
     1621 tctgcgggct ggggtttaga agatgctgca ctgtacgcaa ataccatcac cgtaactaac
     1681 gacggtctgt tggatattaa cgttgatcag ttcgatgcta acccgttcca ggccgatacc
     1741 ctgaatctga ccagtaccac tgatactaac ggcaacattc acgctggtgt attcgatatc
     1801 catagcagtg attacgtaat ggataccgat ctggtcaacg atcgtaccaa cgatactacc
     1861 aagtcaaact acggttatgg cttaatcgca atgaactctg atggtcacct gactattaac
     1921 ggtaacggcg ataacgacaa cactgcttct atcgaagctg gtcagaacga agttgataac
     1981 aacggtgacc atgttgcagc cgcgaccggt aactacaaag ttcgtatcga caacgctact
     2041 ggtgctggtt ctatcgctga ctacaacggc aacgagctga tctacgtcaa cgacaaaaac
     2101 agcaacgcga ccttctctgc tgctaacaaa gctgacctgg gtgcatacac ctatcaggct
     2161 gaacagcgcg gtaacaccgt tgttctgcaa cagatggagt tgaccgacta cgctaacatg
     2221 gcgctgagca tcccatctgc gaacaccaat atctggaacc tggaacaaga caccgttggt
     2281 actcgtttga ccaactctcg tcatggcctg gctgataacg gcggcgcatg ggtaagctac
     2341 ttcggtggta acttcaacgg cgacaacggc accatcaact atgatcagga tgttaacggc
     2401 atcatggtcg gtgttgatac caaaattgac ggtaacaacg ctaagtggat cgtcggtgcg
     2461 gctgcaggct tcgctaaagg tgacatgaat gaccgttctg gtcaggtgga tcaagacagc
     2521 cagactgcct acatctactc ttctgctcac ttcgcgaaca acgtctttgt tgatggtagc
     2581 ttgagctact ctcacttcaa caacgacctg tctgcaacca tgagcaacgg tacttacgtt
     2641 gacggtagca ccaactccga cgcttggggc ttcggtttga aagccggtta cgacttcaaa
     2701 ctgggtgatg ctggttacgt gactccttac ggcagcattt ctggtctgtt ccagtctggt
     2761 gatgactacc agctgagcaa cgacatgaaa gttgacggtc agtcttacga cagcatgcgt
     2821 tatgaactgg gtgtagatgc aggttatacc ttcacctaca gcgaagatca ggctctgact
     2881 ccgtacttca aactggctta cgtctacgac gactctaaca acgataacga tgtgaacggt
     2941 gattccatcg ataacggtac tgaagggtct gcggtacgtg ttggtctggg tactcagttc
     3001 agcttcacca agaacttcag cgcctatacc gatgctaact acctcggtgg tggtgacgta
     3061 gatcaagact ggtccgcgaa cgtgggtgtt aaatatacct ggtaatattc ttcactccga
     3121 agaaatactg gtaatttaat ctaaataatg cccgtcaagg atttgacggg cattactgca
     3181 aaggacgcgc aaatgttatc tgtagttaaa cctcttcagg aatttggtaa gctcgataaa
     3241 tgtttgtcca gatacggtac gcgcttcgag tttaataatg aaaagcaagt tatattttcc
     3301 agtgatgtca ataacgaaga tactttcgtt attttagagg gagttatctc tctgcgtaga
     3361 gaagaaaacg tacttatcgg tattacccag gctccttata ttatggggct ggctgatggt
     3421 ttaatgaaaa acgatatacc atacaaatta atatc
//
LOCUS       DBFIFS010000316         3439 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.317, whole genome shotgun sequence.
ACCESSION   DBFIFS010000316 DBFIFS010000000
VERSION     DBFIFS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.317"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            111..1214
                     /gene="proB"
                     /locus_tag="N7A07_004604"
     CDS             111..1214
                     /gene="proB"
                     /locus_tag="N7A07_004604"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU2479110.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1226..2479
                     /gene="proA"
                     /locus_tag="N7A07_004605"
     CDS             1226..2479
                     /gene="proA"
                     /locus_tag="N7A07_004605"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU2479111.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2594..2669
                     /locus_tag="N7A07_004606"
     tRNA            2594..2669
                     /locus_tag="N7A07_004606"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2627..2629,aa:Thr,seq:cgt)
     gene            complement(2684..>3439)
                     /locus_tag="N7A07_004607"
     CDS             complement(2684..>3439)
                     /locus_tag="N7A07_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU2479112.1"
                     /translation="TPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKN
                     KRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINP
                     EESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDAN
                     TAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAP
                     NHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          794 a          878 c          940 g          827 t
ORIGIN      
        1 cccgcgcaac aaaacgccat gctttgctcg cagatggttg gcaaccgacg acagtcctgc
       61 taaaacgttc gtttgatatc atttttccta aaattgaatg gcagagaatc atgagtgaca
      121 gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg cgccgcctga
      181 accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc
      241 ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg ggttacccgg
      301 aactgccagc gactatcgcc tcgaaacaac tgctggcggc ggtagggcag agtcgactga
      361 ttcaactgtg ggaacagctg ttttcgattt atggcattca cgtcgggcaa atgctgctga
      421 ctcgtgctga tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt
      481 tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct acggcagaga
      541 ttaaagtcgg cgataacgac aacctttctg cactggcggc gattctggcg ggtgccgata
      601 aactgttgtt actgaccgat caaaaaggtt tgtataccgc tgacccgcgc agcaatccgc
      661 aggcagaact gattaaagat gtttacggca ttgatgacgc actgcgcgcg attgctggtg
      721 acagcgtttc aggcctcgga actggcggca tgagtaccaa attgcaggcc gctgacgtgg
      781 cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc gttattggtg
      841 atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa
      901 accgtaaacg ctggattttc ggtgcgccgc ctgcgggtga aatcacggta gatgaagggg
      961 caaccgccgc cattcttgaa cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga
     1021 ctggcaactt ctcgcgtggt gaagtcatcc gcatttgtaa cctcgaaggt cgcgatatcg
     1081 cccacggcgt cagtcgttac aacagcgatg cattacgccg tattgccgga caccactcgc
     1141 aagaaattga tgcaatactg ggatatgaat acggcccggt tgccgttcac cgtgatgaca
     1201 tgatcacccg ttaaggagca ggctgatgct ggaacaaatg ggcattgccg cgaagcaagc
     1261 ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc
     1321 cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct aacgcccagg atgttgctga
     1381 cgcgcgagcc aatggcctta gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg
     1441 gctgaaaggc attgccgacg atgtacgtca ggtgtgcaac ctcgccgatc cagtggggca
     1501 ggtaatcgat ggcggcgtac tggacagcgg cctgcgtctt gagcgtcgtc gcgtaccgct
     1561 gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct
     1621 gtgcctgaaa accggtaacg cggtgatcct gcgtggtggc aaagaaacct gtcgcactaa
     1681 cgcggcaacg gtggtggtga ttcaggacgc cctgaaatcc tgtggcttac cggcgggtgc
     1741 cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa
     1801 atacatcgac atgctgatcc cgcgcggcgg ggctggtttg cataaactgt gccgcgagca
     1861 gtcgacgatc ccggtgatca caggtggtat aggcgtatgc catatttatg ttgatgaaag
     1921 tgcagagatt gctgaagccc tgaaagtaat cgtcaatgcg aaaactcagc gtccgagcac
     1981 atgtaatacg gtagaaacgt tgctggtgaa taaaaacatc gcctatagct tcctgcccgc
     2041 attaagcaaa caaatggcgg aaagcggcgt gacgttacac gcagatgcat ctgcgctggc
     2101 gcagttgcag acaggccctg cgaaggtggt ggcggttaaa gccgaagagt atgacgatga
     2161 gtttctgtca ttagatttga acgtcaaaat cgtcagcgat cttgacgatg ccatcgccca
     2221 tattcgtgaa cacggcacgc aacactccga tgcgatcctg acccgcgata tgcgcaacgc
     2281 ccagcgtttt gttaacgaag tggattcgtc cgctgtttac gttaacgcct ctacgcgttt
     2341 taccgacggc ggccagtttg gactgggtgc ggaagtggcg gtaagcacac aaaaactcca
     2401 cgcgcgtggc ccaatggggc tggaagcact gaccacttac aagtggatcg gcattggtga
     2461 ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccgttt gattcacaag
     2521 gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac gttcgtgaac
     2581 atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta atgcgaaggt
     2641 cgtaggttcg actcctatta tcggcaccat ttaaatcaat aagttacaca tcattagtac
     2701 cttccttatt ttttgactgg gacaaatttg ggaccgatgg gttcaggatc gagtctattt
     2761 gccgtgcgtg ttcggtaagg tgattaggtg caaggtgagc atatcgacga accatttcga
     2821 tagactccca gcctcccatt tcctgtaaca ctgacaacgg gactccggct tgaaccagcc
     2881 aacttgccca ggtgtgtctc aagtcgtgaa atctgaaatc atcaatacca gcccgtctca
     2941 gcgccgcttt ccaggctgtg tttgcgtcat accgcatctt ccttactgtt ggcgctttcg
     3001 ttccgtctgg tttggtacag ctttccttgt acacaaatac ccaacggtga tgattaccga
     3061 tttgtttttt caatacgcga catgcagtat cattcagcgc aacgccaatt gcgcggtttg
     3121 atttactctc ttccgggttt atccatgcca cccggcgctg catatctatt tgttgccatt
     3181 caaggttgat gatgttcgag cgtcttaagc ctgttgccag tgcaaattca acaacagact
     3241 ttaatggctc cggacattca tcaatcagcc tttgtgcttc atggggctcc agccagcgga
     3301 tccgtttatt ctttggttga ggcactttaa taattggtgc cttatcgagc attttccatt
     3361 cacgctctgc ggctcttagc agggccttta taaatgaaag atgcgtagcc ttcgttgcaa
     3421 cggacgctgg ttttggcgt
//
LOCUS       DBFIFS010000317         3392 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.318, whole genome shotgun sequence.
ACCESSION   DBFIFS010000317 DBFIFS010000000
VERSION     DBFIFS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.318"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(151..870)
                     /gene="dcuR"
                     /locus_tag="N7A07_004608"
     CDS             complement(151..870)
                     /gene="dcuR"
                     /locus_tag="N7A07_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU2479113.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(867..2498)
                     /locus_tag="N7A07_004609"
     CDS             complement(867..2498)
                     /locus_tag="N7A07_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU2479114.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQGATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2679..2909
                     /gene="yjdI"
                     /locus_tag="N7A07_004610"
     CDS             2679..2909
                     /gene="yjdI"
                     /locus_tag="N7A07_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU2479115.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2921..3193
                     /locus_tag="N7A07_004611"
     CDS             2921..3193
                     /locus_tag="N7A07_004611"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2479116.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          869 a          777 c          849 g          897 t
ORIGIN      
        1 cgttcacctg aagagatatt aatttttagc gatgatggag ggataattat atttgatctg
       61 gcacaagttt tactgatgaa ggatgtaact tgtaccaggg gtattttgca ttacggtaat
      121 aattattact tacagataac cagcgatccg ttattggcaa tattgtttca gtagtgagta
      181 gtgttctgcc tgaatacggt aacggtaaac tggacgcccc gtgacgccat aatggatact
      241 ggtgaacaag atgtggcagt tgaccagcca gataaggtat ttacggcagg aaacacgcga
      301 aatgttaacc tcgttggcta gctcgtcggt tgaaaactca tagtcctgat gcgcgtcaat
      361 ccactggcac agtgtgcgta acgtctgcgg cgttaagcct tttggcaagc gacgaggatc
      421 ctgctcgttg gagctgctgc cgtggattag ctgatcaagc tcggcctggt cataatactg
      481 atgtttttcc agcgccattt tcttttgccg ccagccggtg agcgcctctt caaagcggga
      541 agcctggaag ggtttgatca ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt
      601 tgccgcatcg gctgcggagg agatgacaat cacatcactt ttgcaacgcg cgttatgcag
      661 gacaggcagt aaatcgagcc cgttctcttt ttgcatatag atatcgagca atatcaggtc
      721 gataggcgta tcgctattga agataatctc tttggctttc tccagcgtcg aggctgttcc
      781 acagcattga aagcctggga tttgtgctac gtatcggcga ttcagctccg cgaccattgc
      841 gtcgtcatcg ataattaata cattgatcat ctgttcgacc tctccccgtc ccagggtatc
      901 tggacaaaaa attgtgtgaa aatcccgggt tccgattcca cggcgatgct gccgccgaga
      961 ttttctacct gttgtttgac aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag
     1021 acacctttgt caaaaatgtg atcgattttg tcgggtgcga tccccggtcc atcatcgtta
     1081 acttcgcagt gcagccagcc gtgacggtag tgcaatgtta cgctaatttc gccaccgggt
     1141 tccggcccta atgcttccag cgcgttttct atcagatttc ccaatgtggt aatcagcgtc
     1201 gcgccctggt cctcactgcc gctgtctggc agctggcttt cactgtttaa aatcagcgta
     1261 tggcctaaat cggtcgcgcg gttaatcttg ctgattaaaa aaccagcgat aaccggagat
     1321 ttgatcttac ccagcagaga gccaatctct tcctgatagt tattggctgt tttgagaatg
     1381 taatcttcca actgcttata actcttcaga tgcaataatc cgagaatcac atgcaattta
     1441 ttcataaatt cgtgggatcg ttcacgaagt gcatcagcat agttgaccag accgtcgagt
     1501 cgctgcatca gtttacgaac ttcagttttg tccctgaagg ttgaaatggc accgatgata
     1561 acgccattac tgcgcaccgg aacggtgttg atcagtaata gccggtcttt aatcgtaatc
     1621 tcttcgtcgc ggcgcggcgt accgtcgcgt aacacttccg agacatctac cacctgtgac
     1681 catgagtggc tgagcgtcga cagtttctca tcgtcctgcg acttacggta attcagcaat
     1741 tcttgtgcgg catcgttgat cagcgtgacc tcgccgcgat cgtccacggc aacgacgcct
     1801 tctttgatag actgcaacat ggcctggcgt tgctcaaaca gcgtggagat ttcgtagggt
     1861 tccaggccga aaaggatttt tttcagtacc ttaaccagaa tgcaggtgcc aatcagtccg
     1921 accagcatgc caaataatac cgaccagata atgctccagc gactgtcatt gatctgttgg
     1981 gtcacgcggc ttaactcaag gccgatcgcc accacgccaa tttgtttatg attttcatcg
     2041 tagatggggg taaatacgcg taaagcctgc gccagaaaac cgcgattgat agcgacattt
     2101 tcttcgccat tcagcgcttt aaggatgtca tcacctttaa atggctgacc aatacgctgg
     2161 gcttcaggat gcgagtagcg aagactttgc atatcggtaa cgacaataaa cagcagatcg
     2221 ttgcgtttgc gcacggcttc cgcgatggcc tggatgccgc tctcctgcgg ttttttctgc
     2281 aagccctgac ggatttccgg cgagtcggcg agagtacgcg ccactgccag tgccttgttg
     2341 gctagcccgt ctcgcgtcat atcactgatt tgcgagaagt aaatcagatg caccaccaat
     2401 agcaccgaga acagtaccgc gctgaccatt aagatcactg tggtactcaa tttcatcgga
     2461 cgtttgcgta acatgcggta gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt
     2521 tcttaagcat tatctctgat gaggcgggta attcaaaggg agtaagaatg attggctata
     2581 taggggaaga gactctggca acggaaactg ccagtgctgt atgaagattc cagggctatg
     2641 cttatagtga aaaccatact gatgagagag ggaaggtcat ggatcaggcg ctactggacg
     2701 ggggttatcg ctgttatacc ggcgaaaaga tcgatgtcta tttcaacact gcgatatgtc
     2761 agcattctgg caattgcgta cgtggcaacg gcaagttatt taatctcaaa cgaaagccgt
     2821 ggatcatgcc ggatgaagtc gacgttgcca ctgtggttaa agtgattgat acgtgcccga
     2881 gtggtgcgct gaaataccgt cataaataag cgagggtaaa atggaaatac gcgaaggcca
     2941 caataaattt tacattaatg acgaacaagg caagcaaatc gctgaaattg tctttgtgcc
     3001 gaccggagag aatttagcga ttatcgaaca taccgatgtc gatgaaagcc tgaaagggca
     3061 agggattggt aaacagctgg ttgcgaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa
     3121 aattatccca ttatgtccat ttgcgaaaca cgaatttgat aaaacgcggg agtatgatga
     3181 tattcgcagt tgatggaaga gtacagattc acgatatttt tcatgctctc cgcggtgtga
     3241 tgcaggagag catctgaagg gtagggggat gcacaaagaa tgggcagaga gcgcgttttt
     3301 ttgtcccaag tcatcccctt tactgagcaa aaaaaagaat atctcctata tgagaatcat
     3361 caatcggggt taataaattt tgcgtcccca ga
//
LOCUS       DBFIFS010000318         3372 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.319, whole genome shotgun sequence.
ACCESSION   DBFIFS010000318 DBFIFS010000000
VERSION     DBFIFS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.319"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..631
                     /locus_tag="N7A07_004612"
     CDS             <1..631
                     /locus_tag="N7A07_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU2479117.1"
                     /translation="DLLADDTALRALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDT
                     QAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIGGKERYFFGNILWQTRALMDR
                     AIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKG
                     DYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            730..1743
                     /gene="ybjS"
                     /locus_tag="N7A07_004613"
     CDS             730..1743
                     /gene="ybjS"
                     /locus_tag="N7A07_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU2479118.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1740..2570)
                     /gene="amiD"
                     /locus_tag="N7A07_004614"
     CDS             complement(1740..2570)
                     /gene="amiD"
                     /locus_tag="N7A07_004614"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU2479119.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2567..2890)
                     /gene="ybjQ"
                     /locus_tag="N7A07_004615"
     CDS             complement(2567..2890)
                     /gene="ybjQ"
                     /locus_tag="N7A07_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU2479120.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3101..>3372)
                     /locus_tag="N7A07_004616"
     CDS             complement(3101..>3372)
                     /locus_tag="N7A07_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU2479121.1"
                     /translation="KVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARMIFAYIND
                     KSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          801 a          905 c          849 g          817 t
ORIGIN      
        1 cgatctgctg gcggatgaca ccgcgctacg tgcgctcatc ccacaacggc tgatcgcttt
       61 cgatgacgcg gtacgtcgca ccctgaaaga agaagaaaag ctggtcaact ccagcgactg
      121 gggatacgac actcaggcct ttgcccgctg gcgaccagag tatggttatt ttgccaaaca
      181 ggcgggattt accgttaaaa cgtccgccag ccttgcggct ttatggcagg tggtgaacca
      241 aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac gggcgttgat
      301 ggaccgtgcg atcggtcata aattagcgaa aggccgtccg gagcgcgaat atttgcaaac
      361 tggcgatgcg gtggatagct ggaaagtgat tgtcgttgaa ccggaaaaac aacttacgtt
      421 gttatttggc atgaaagcac cggggctggg acgactgtgt tttaccctgg aagataaagg
      481 cgactatcgt actatcgatg tccgcgcttt ctggcatccg cacggtatgc cggggctgtt
      541 ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg caaaacgaat
      601 cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat tctttcatgt
      661 ttcccattgc atcaaggggg aaatcacgga agaatgcgca cgtcattttc tcaacaacag
      721 tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg aaacgcggta
      781 gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga ggcaatgggt
      841 aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga gctggtctca
      901 tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc cagctttacc
      961 tctccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac tcgccgccta
     1021 ggtgaatggg ctgtcgcctg gggtgtacgt aactttattc atatctcttc tccctccctg
     1081 tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc
     1141 aacgagtttg cccgcagcaa agcggccagc gaagaagtga tcaatatgct ttcgcaggcg
     1201 aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc gcacgataaa
     1261 gtctttatcc cccgtcttgc gcatatgatg caccactacg gcagcattct gttaccgcat
     1321 ggcggcagtg cgctggtgga tatgacctac tatgaaaatg ccgtgcacgc catgtggctg
     1381 gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat caccaacggc
     1441 gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa tattgactgt
     1501 cgtattcgtt ccgtccctta cccgatgctg gatatgatcg cccgcagcat ggagcgttta
     1561 ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctcgaa gcttaatttt
     1621 gactttacgc tggatattac gcgggcacag gaagagttag gttatcagcc ggtcatcacc
     1681 ctggatgaag gtatcgagaa aactgccgcc tggctgcgcg accacggaaa actgccgcgc
     1741 taatcctgcc cgtatttctc cagcaacgct tcggcaatcg cctgagtttc tgcatccgct
     1801 tcgccgttat ataacgtcgg gcggaaatgc atctggaacg ccataatcac gcgccgctgc
     1861 tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag ctccagcaat
     1921 gacgcggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt cacccgctgc
     1981 gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa taatggcccc
     2041 ggatcgtctt tgcgctgcgg ggcgatatcc gcatgtgcca ctacgttttc cggcttgatg
     2101 tgataacggg caataatatc tttcgccagc ggaataagcg cctgaatctg tgccggttca
     2161 aacggggcaa aatatttcac ccccgccgat ttttgccagc cacggttttc cagctcaatg
     2221 ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag
     2281 gccagttctt gttccggcac caattgccag atgcgcggtt taccgttgta tcgcggtggt
     2341 accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc cagcgagcta
     2401 tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata cgccgcctgc
     2461 gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt ttcgcctgcg
     2521 caccctgcca gtaacagagc gacagcgatc agccagaaga ctcttctcat cgacgcgttt
     2581 tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg accgtttcgt
     2641 agtcgatatc aataccgact acggcatcgg cccccagcgc ccgcgcctgg gagccaagct
     2701 cctcaaaggc gatctcccgc gctttacgta gctctttttc gtaggcaccg gagcgtccgc
     2761 caacgatatc gcgaatacca gcaaagaaat cacggaaaat attcgcgcct aaaatcgctt
     2821 cgccagtcac cacaccgcaa tattcaacga tggtctggcc ttccagagtt ggagttgtgg
     2881 aaaattgcat gatcgatctc cttgtttgtt aagccttcga tctctaaagc attatcggac
     2941 tgatatgcca tgttgggaag aaggttttga tcagtttagg aaatcatctc attgtgtcaa
     3001 aaacagctac ataagcctgt acaattatct gtttcgtaac cggatattgg tttcattatg
     3061 gagcaccgct tacctgccct attatgttgt gccggcaaca ttaatgagtg ggaagagtca
     3121 acttatattg taaatgattc ctaaagtctt ccccatcttg cgcctgaggt atataccgct
     3181 ggattgcttt ttggtccgcc gccgcaaaac aagaaagtaa taagatctca atgcttttgt
     3241 cattaatata agcgaagatc attcttgcaa attcgtatat aaacgcccga tcctgagcgc
     3301 ttaatagttt aatacaatat ttatttttaa gagaataaag tatttcaaga aaagcatccg
     3361 cagcaacctt ca
//
LOCUS       DBFIFS010000319         3372 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.320, whole genome shotgun sequence.
ACCESSION   DBFIFS010000319 DBFIFS010000000
VERSION     DBFIFS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.320"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..269)
                     /locus_tag="N7A07_004617"
     CDS             complement(<1..269)
                     /locus_tag="N7A07_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU2479122.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQG"
     gene            complement(272..2851)
                     /locus_tag="N7A07_004618"
     CDS             complement(272..2851)
                     /locus_tag="N7A07_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2479123.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEEKIAAQAQTFEEKLASQSVVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR
                     GTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKT
                     FSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRD
                     AGG"
     gene            complement(2797..3018)
                     /locus_tag="N7A07_004619"
     CDS             complement(2797..3018)
                     /locus_tag="N7A07_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479124.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(3063..>3372)
                     /locus_tag="N7A07_004620"
     CDS             complement(3063..>3372)
                     /locus_tag="N7A07_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU2479125.1"
                     /translation="SFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLE
                     MQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG
                     "
BASE COUNT          670 a         1119 c          824 g          759 t
ORIGIN      
        1 tccccctgaa tcagttcttc ccgctccgca agatccggat tccggtacag catcccggtc
       61 agtcgcatgg cagccccctt catccgggtt aatgcctcgc ccgggatcag ttcaccgtcc
      121 tcacgaatca ccttatcccg gctgccctga atgtaggcca gcagcacggc ggtagcctga
      181 cgaaccttgt ccatcagcat gtcatcatcc gcgtcatggt caacacgcag atgtgccttg
      241 atctcttcca gtgtcagtaa tgccgtcatt ttcagcctcc tgcatcccgc ccacgttttg
      301 cagccagggt ccaggctgat gaatgagctt ctccgggttt atcttcggtc atactgttgc
      361 agtgccacag cgagcccccc cacgtcaccg tatcgccggg gtggtaggtt tcaccggctc
      421 tgaacacacc gcggtagagc atcaccggca gggaaaatgt tttttccgta cgctggccac
      481 tgctctgccg gaccaccaca gagaacaacc gctcacccgt catgctgacg tcaatatccg
      541 ccaccccgtc aaccaggcat tcccatcccc gcatcccgtg cgttttttca tacgcccgcc
      601 agagtccgcc ctggtgtgtg gcatacgtgc cccggggaaa ggatttttga tcgtcaatgg
      661 cggggagtat ttccagtgcc gtggcatcac gcccgtcctg cggagccggc agggcactca
      721 ccgcatccag aaccgccttc tgcagaacat ccggatcgta gtcacgacca tcacgcggaa
      781 caggaatatg gcttaccgcc tccttcacca tctgttcaag catcggacgc acatcatccg
      841 gggtgagact tttaccgtct gccggctgcg gaatatttgc gaccgcatca ttcaccgcct
      901 tctgcagtac ttccggatca tagtcacgac cgtcgcgcgg aacagggata tggcttaccg
      961 cctccttcac catctgttca agcatcggac gcacatcatc cggggtgaga cttttaccgt
     1021 ccgccggctg cggaatattt gcgaccgcat cattcaccgc cttctgcagt acttccggat
     1081 cgtagtcacg accatcacgc ggaacaggga tatggcttac agcctccttc accatctgtt
     1141 caagcatcgg acgcacatca tccggggtga gacttttacc gtccgccggc tgcggaatat
     1201 ttgcgaccgc atcattcacc gcctgctgca gtacatccgg atcataatca cgaccatcac
     1261 gcggtaccgg aatggccccc acagcgtcat ccaccatcgc ctgcagaacc ggacgcacct
     1321 catccaccgt cacatgcttc tgtaatacca cagactgaga agccagtttt tcttcaaacg
     1381 tttgtgcctg cgcggctatt ttctcctcaa atgtgcgctg taaatccgcc agcactgtgg
     1441 aaaattcttc tcccagtgca cgaataatgg acagttcccg ttccgtcatt ttctcagtat
     1501 cccccctgaa catcgctttc accgcatcat gctctgtttc actgattgcc ttattaccgt
     1561 cagatgcgcc gtcaggcagc tgtgatgaaa ctgttttccc ggcagacgcg aacggatcct
     1621 cacgggcatc acgacgggac agcgcctcca gactgtagtt ctgctgctga agatacagtg
     1681 catcaccgcc ggccaggggc ggcaggttct cccgtttacg ggcctcattg ggcgtgagaa
     1741 gcgtattttt caccgcatcc cccagcgttt tcatgcgccg ctcactgtcc attctcagca
     1801 gcgtggtgac atcaaattct gtactctcgt tttcccccgt ttccagcgcc tcatccagta
     1861 acagttcaat ggactcaatc agcgtctgca ggcactggga ataatactgc tgctccagcg
     1921 cctccacgtt gtcactggaa ggcggttgtc ccacgccaat cttgtaggcc gggacacgga
     1981 acaccgaaca gacaatttca gcggtcatct tcagttgttc caccgtctgc gcatccaccg
     2041 gtgaaaacgt cgtggggttg tattttgccc cgttgctcag aatggccgtt ttccccgcat
     2101 tttcgcctgt atacccgctg tcccagttgc tcttcagttt tttcgcattt tcttccgtaa
     2161 tactgccggg gatctcaatc accccggacg gcctgccgcc atttctgaaa aaagacgtcg
     2221 aattttcctg aatatgatgc ccctgcgtgg ccgccagccc ggcggcatac accggcggca
     2281 atcctataag cggatgaaaa aaacagttaa accggtcgtg gatcacttcc cgggcaggca
     2341 ccgtcaccgc ctccgtgatc ccgcagttcc ggtccggcgt gatgcggtag aacacctcgc
     2401 cgtcatccgc caccagaggt tcaacccggc tccagtccag aatacgcagt tctttgatct
     2461 gcccccgggc attacggatt ttcagcacca ccgtattgcc atgacgcagt ttggcgttca
     2521 gccacagttc aaaaaactgg atgcggttct gctgggcgtt gggacgacga cagaggcggg
     2581 caatatcccc ccggcgcgtt tccctgcgta tcccatgcgc atccgtctgc ataagacgca
     2641 gccgcatttt ggcgatatcc tgggatatca gcgaaataca tgcaaacacc gcatgaaagg
     2701 agaggacggc ttcaggatcg gctttcacgc cctgctgcca ggcgccggaa aagggctcag
     2761 ccaccgcctg aaacaggctg gtccagcccg cctctcttac gtcacgtcct gatttctggt
     2821 tttttcgggt tcgccgtaaa aggttccaca ttcgccatgc tccgcatcac gtttcttttt
     2881 ctgacctgcc ggacgtcgca ccgtgatgta ctccgccttt cccaggcgaa ccagcacctc
     2941 cgcacacggc tgtgccacat cacggatatc cccggcccgg gcatcatgcg tgccctgcag
     3001 atatcggatc tttgccataa cctgttacgg gaggcgcacg cctcccgtcc tccttatcag
     3061 actcagccgc cggacgcact gccgtagttc actccggtga tcaccgccac cgccgcagta
     3121 cggcgacgac gccagttgat ccagcgctcc gcacggatgg ccacgctgcc tgtctggaac
     3181 atggaaacca gctccaccgg ggacggcgtg gtactgtcgc cgcccggctc agactgcatt
     3241 tccagtgatg cctcgcggga catatccact gccacgccgc cgtcatccgc cagataaata
     3301 tccggggcat tcaccagcac cagctggtca cccacgtact gggagacaat caccggcagc
     3361 ccctggaagg ag
//
LOCUS       DBFIFS010000320         3341 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.321, whole genome shotgun sequence.
ACCESSION   DBFIFS010000320 DBFIFS010000000
VERSION     DBFIFS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.321"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..910
                     /locus_tag="N7A07_004621"
     CDS             <1..910
                     /locus_tag="N7A07_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HGU2479126.1"
                     /translation="NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKR
                     RDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSS
                     CQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLF
                     ARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYR
                     IKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPH
                     HYNGGLTPEESENRYHFYCKTVASIT"
     gene            919..1071
                     /locus_tag="N7A07_004622"
                     /pseudo
     CDS             919..1071
                     /locus_tag="N7A07_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            1161..1535
                     /locus_tag="N7A07_004623"
     CDS             1161..1535
                     /locus_tag="N7A07_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU2479127.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1532..2023
                     /locus_tag="N7A07_004624"
     CDS             1532..2023
                     /locus_tag="N7A07_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2479128.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            2035..2232
                     /locus_tag="N7A07_004625"
     CDS             2035..2232
                     /locus_tag="N7A07_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU2479129.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            2317..2658
                     /locus_tag="N7A07_004626"
     CDS             2317..2658
                     /locus_tag="N7A07_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479130.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            2719..2862
                     /locus_tag="N7A07_004627"
     CDS             2719..2862
                     /locus_tag="N7A07_004627"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479131.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            2856..3275
                     /locus_tag="N7A07_004628"
                     /pseudo
     CDS             2856..3275
                     /locus_tag="N7A07_004628"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          888 a          864 c          830 g          759 t
ORIGIN      
        1 caaccgcgct cttgatgtcc gactcgctga acggttcacg atagttgcca gactgagtga
       61 cagccactca gttgtcagcc tctgttctgc tctggaaata caccgcagca gttaccagta
      121 ctggcgaaaa cgacgcgata cggttaatcc ggcgcgagtc aggttgtgca gcgaaatacg
      181 ccgggcgtgg aaccaaagta ggggctctgc gggggcgcgc acgctggctg aaatgctgac
      241 ccaaaacggc gtcccgatga gccgttaccg tgccgggcgt ctgatgaaat atctgaacct
      301 gagcagttgt cagcccggaa aacatcagta caaaaatgct cgtcaggaac atacctgcct
      361 gccgaatctg cttgagcgcc agttcgctgt gcccgagcca gatagggtat ggtgcggaga
      421 tattacgtat atctgggcag gaaatcgctg gtgctatctg gcggtcgtca tggatctttt
      481 tgcccgcagg gttatcggct ggagcctgtc agcgaatgcc gatactgccc tgataagcag
      541 tgccctgcgg atggcgtatg aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga
      601 ccagggaagt caatatacag gactgaaata tcaacaactt ctctggcgtt acaggataaa
      661 gcaaagtgtc agccgacggg gaaactgctg ggataacagt ccaatggaac gcttcttccg
      721 cagtctgaaa acagaatggg tgccaaccga tggctacaca ggcaaagacg tggcccggca
      781 gcaaatcagc agttatattc tgaattatta caatagcgtc aggcctcacc attataacgg
      841 agggctgacg ccggaagagt cagagaacag atatcatttt tactgtaaaa ccgtggccag
      901 tattacttga ccactacact ggaactcatg ctcaccagcg gtgagctgaa tccccgccat
      961 cagcataccg tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcggcagt
     1021 cgaggttacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta aatcatcaga
     1081 ataaccttct gctccggcct ggtgttttca ccacgccact tccccatttc tcatctctgc
     1141 atatcaggaa aaactttagt atgaaaacat tacccgatac acacgtacgg gaggcatcgc
     1201 gctgcccgtc tcccatcacc atctggcaga cactgcttgg ccgtctgctg gaccagcact
     1261 acggcctcac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg
     1321 aggcaggtat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gtgctggtgc
     1381 gtaccgacca gccgggattc agcgcctgta cccgctctca gttaataaac agcatcgata
     1441 tcctccgggc tcgcagggcg accggcctga tgacccgcga caattacaga acggtaaata
     1501 acattaccct gggtaagcat ccggaggcga aatgatgaaa ctggccctga cactggaagc
     1561 cgacagcgtt aacgtacagg cactgaatat ggggcgaatt gtcgttgaca ttgatggtgt
     1621 taatctcgct gaactgatta acatggtctg cgataacggc tactcccttc gtgttgttga
     1681 tgaatctgac cggacctcag cagactgcac gccaccattt actgccctta ccggcatacg
     1741 ctgcagtacc gcacatatca cggaaacgga caacgcctgg ctgtactcgc tgtcacacca
     1801 gaccagtgac ttcggtgaat cagaatggat tcattttacc ggtaacggct atctgttgcg
     1861 taccgatgcg tggtcgtacc cggttctgcg gcttaaacgc ctgggtctgt caaaaacgtt
     1921 ccgtcgtctg gtcgtcacac tcatccggcg ttatggtgtc agtctcattc atctggatgc
     1981 cagtgccgga tgtctgccag gtttacccac ttttaattgg taactaggaa caacatgaca
     2041 tcattaaccc cggaagcagc actggatatt ctgattgcgt ggctgcagga caatatcgac
     2101 agcgaatccg gaattatctt cgacaacgat gaggataaaa cggattcggc agcattgttg
     2161 ccctgtatcg aacaggtcag ggaagatgtc cgtaccctgc gccaactgca gcttctgcaa
     2221 cagaaccggt gagtctcact caccatactt cattccactg acgccagcct gaacagggct
     2281 ggcgttttcg tttataaacc aaaaggaaaa ccgataatgt caggcatcat aatctcccgt
     2341 ccggaagtgg acaccgggca tacggacgtt atctgctcca ccagtatccg acacgttgtg
     2401 acggtacgga atgcagcact gcaacaaacc gaaacgctta tccggcaact ggcagaaatc
     2461 tcagtcctga cagcagcaga cattggcggc aaaaccgccc tggaccgggc gatgaaacag
     2521 gattttcact gcggctgctg gctgatggag aaacctgaaa ccgcaatgaa agccatcacc
     2581 cgcaacctcg actgcgaaat ctggcgtgac ctgatgcaac gctccgggat gctgagtctg
     2641 atggatgcac agggctagga tacatggtac cgctcactgg agtacgataa tattccggaa
     2701 atcagcgagg cgaatatcat gaatgcattc aaacaactgc accagagtaa ggatgaagtg
     2761 tttgagcgtg gggtgatcaa cgtcttcaga gggccgaacg gaattacaaa accaattccc
     2821 cctgtaaatt cggcagtaaa attatcgtca acaacttggt gagatgggac agatggagat
     2881 ttcatcttat cagtggaatg caggcagatc gcttagctga cctggaaaga atgttgcatc
     2941 tgctcagcgg taaaccgctc cccaacaacc gagggaatat caccattaat ctggatgacc
     3001 acctacagtc cgttcagggt aaagagcgct atgaagacaa gtaaatactt taagaagggc
     3061 ccgcacacat cacattcaga aagccagagc tggttgacag gcttaatgat attattgcca
     3121 gacactacct aggaatgtta ccttcacaaa aatctctaag aattgattca attgtgtatt
     3181 ttagcctcat tccctcatgt aaagtcattt gggggttatt atgggggtca atcatttatc
     3241 caacacgcat taatcattta aaatcaatgc attaataatc atattcgact cctgtgatct
     3301 ccgccaaact tcctcttctg aactctcccg acgtcaacaa a
//
LOCUS       DBFIFS010000321         3326 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.322, whole genome shotgun sequence.
ACCESSION   DBFIFS010000321 DBFIFS010000000
VERSION     DBFIFS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.322"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..384
                     /locus_tag="N7A07_004629"
     CDS             <1..384
                     /locus_tag="N7A07_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2479132.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="N7A07_004630"
     CDS             418..1134
                     /locus_tag="N7A07_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2479133.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1131..1448
                     /locus_tag="N7A07_004631"
     CDS             1131..1448
                     /locus_tag="N7A07_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2479134.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1445..1747
                     /locus_tag="N7A07_004632"
     CDS             1445..1747
                     /locus_tag="N7A07_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU2479135.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1737..2054
                     /locus_tag="N7A07_004633"
     CDS             1737..2054
                     /locus_tag="N7A07_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU2479136.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2008..2325
                     /locus_tag="N7A07_004634"
     CDS             2008..2325
                     /locus_tag="N7A07_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479137.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2312..2749
                     /locus_tag="N7A07_004635"
     CDS             2312..2749
                     /locus_tag="N7A07_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2479138.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2751..2942
                     /locus_tag="N7A07_004636"
     CDS             2751..2942
                     /locus_tag="N7A07_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479139.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2945..>3326
                     /locus_tag="N7A07_004637"
     CDS             2945..>3326
                     /locus_tag="N7A07_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479140.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          920 a          693 c          992 g          721 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtccagga aacggctgca
      901 caggaagaaa ttaaaacaga agcggtggag gacattgtga agttgcagca atcgttcact
      961 gaagcgaaag cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcgaaaa gtaatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac
     1081 aaacacaggg atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct
     1141 ggaagaagtg gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgagag
     1201 tcgaaagcat cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg
     1261 aactgggtat cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc
     1321 gtgatattga gctggcgtgg caactgcatc agcgtggtgt accccgacgg gaaattgccg
     1381 aaaagtttgg gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc
     1441 aggagtgagg gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg
     1501 cgaggcattt cacaaaacgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc
     1561 ggcagtactt ccggacgggc tgactcagcc acactacatg gatatttgca tggcaatgct
     1621 ccgttgcgtg gatgcggttt acatgctgaa aggctggcag cagtcggcag gtgcaggggc
     1681 tgagctggca ctggaggaga aaccgggcca tgcggtgatt tttcaggagg tgggcagtga
     1741 atattgaccc ggcgataacg attgatatgg ccctgaacgc cggcctggca cttcttggtt
     1801 atttctacat tatgttctgc agcggacgat ggctgtcact gttgttcatg aaaaaatgga
     1861 ataaacgccg taagcaggag caacgccaga aggcaatgga tgcatttttc gaagccttcg
     1921 gaattgacgg catggaacca ggggatccag ctcgcgcaat cagcagaggg ggtgtagtaa
     1981 tccttgtata tcggagtgaa gagaaaaatg acgatcacaa aacaacgtgt agaagaaatc
     2041 atatcccgca ttgaaatgta tggacatggt gcagggtata tcgcagacga ggttaatgat
     2101 ctggctatac tggcgctgaa tttatcaaat atcgcgaacc tgaagcgata cgagcttgat
     2161 atgggaggtt gcgactcgtg cggtcaggat tgtggcgctg atatgactga agatcctgat
     2221 ggtgattatg tcctgtttga tgacgtggtt aagttgtttg aatttgatac aaccactcaa
     2281 aagttagaaa tcccggcaaa ggaggcagcc agtgggcaag attgactatc aggcactgcg
     2341 tgaggcagca caaaactatc aatcgacgct ggcgtggtat caggctaccc cggacagccc
     2401 aaatgctgaa cgggattgtg atgcggctct tgctgcgttt aagcgtcaca tccgtcatcg
     2461 ggaagcggat attatcgctg atttgctgga tggactggaa gaagcaaaat cacaactcaa
     2521 cgagcagcgt gagtattacg aaggcgttat ctctgatggg agcaagcgta ttgctgaact
     2581 ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac acccgataaa
     2641 tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt acctgattga
     2701 cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagagtgag atgaacggac
     2761 aaatatcaat tgttcgacca ggagcatgtg acgatcgcga aatacgaatg attattcgtc
     2821 tggcgatggg gaaaacaata actgctctca ttactccaga aaatctcgca ttagcattaa
     2881 caggaaagtc agacatgcca gtagagctaa agctgcgaaa tgttgagatt aaggtgaaat
     2941 agctatgacc actattacca aagatcgact gctgacaatc cagcattggc gcgaaacata
     3001 cggaccgggt agcaacgttg tgcttccagc agaagaagcg gaagagctgg cacggattgc
     3061 gctggcatca ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa
     3121 gcgtttcgga gaatctgtag atcgccgcag agcaaaaaat ggcgatagag attacatggt
     3181 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     3241 aaactttcgg gaaaataagg gttcgtcaac caataatttt cgggaaatct cggaaacgtc
     3301 aaccaactat ccggtaactc cggatg
//
LOCUS       DBFIFS010000322         3322 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.323, whole genome shotgun sequence.
ACCESSION   DBFIFS010000322 DBFIFS010000000
VERSION     DBFIFS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.323"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..426
                     /locus_tag="N7A07_004638"
     CDS             <1..426
                     /locus_tag="N7A07_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU2479141.1"
                     /translation="NDARTLFEFTSGVNVIESPIIYRDESMRTACSPDGLCSDGNGLE
                     LKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYV
                     VVERDEKYMASFDEMVPEFIEKMDEALAEIGFVFGEQWR"
     gene            423..605
                     /locus_tag="N7A07_004639"
     CDS             423..605
                     /locus_tag="N7A07_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2479142.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            578..769
                     /locus_tag="N7A07_004640"
     CDS             578..769
                     /locus_tag="N7A07_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2479143.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            846..1061
                     /locus_tag="N7A07_004641"
     CDS             846..1061
                     /locus_tag="N7A07_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2479144.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1160..1381
                     /locus_tag="N7A07_004642"
     CDS             1160..1381
                     /locus_tag="N7A07_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2479145.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1378..2325
                     /locus_tag="N7A07_004643"
     CDS             1378..2325
                     /locus_tag="N7A07_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2479146.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     EGIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            2327..2503
                     /locus_tag="N7A07_004644"
     CDS             2327..2503
                     /locus_tag="N7A07_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479147.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            2837..3193
                     /locus_tag="N7A07_004645"
     CDS             2837..3193
                     /locus_tag="N7A07_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479148.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            3190..>3322
                     /locus_tag="N7A07_004646"
     CDS             3190..>3322
                     /locus_tag="N7A07_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479149.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     "
BASE COUNT          982 a          688 c          868 g          784 t
ORIGIN      
        1 aacgacgcca gaaccctgtt tgagttcact tccggcgtga atgttattga atccccgatc
       61 atctatcgcg acgaaagtat gcgtaccgcc tgctctcccg atggtttatg cagtgacggc
      121 aatggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt ccggctcggt
      181 ggtttcgagg ccataaagtc ggcttacatg gcccaggtgc agtacagcat gtgggtgaca
      241 cgaaaagatg cctggtactt tgccaactat gacccacgta tgaagcgtga aggactgcat
      301 tatgtcgtgg ttgagcggga tgaaaagtac atggcgagtt ttgacgagat ggtgccggag
      361 ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatttgg ggagcaatgg
      421 cgatgaagca tcctcacgat aatatccggg taggtgcgat cactttcgtc tactccgtta
      481 caaagcgagg ctgggtattt cccggccttt ctgttatcag aaatcccctg aaagcacagc
      541 ggctggctga ggagataaat aataaacggg gagctgtatg cacaaagcat ctcccgttga
      601 gttaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca ggtttgtgcc
      661 aataccagta gaaacagacg aagaatttca tacgttagcc gcatcccttt cacaaaagct
      721 ggaaatgatg gcggcgaaag cagaagcaaa cgagagagac ccggcatgac aacaacagaa
      781 tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga catgggactt
      841 gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag gctaattctg
      901 aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt tgccgtagcg
      961 tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact catttaagtc
     1021 tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa gcaaaatcat
     1081 cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta ttaaccaaca
     1141 attcagggat taatgaaaga tggcagatat cattgattca gcatcagaaa ttgaagaatt
     1201 acagcgcaat acagcaataa aaatgcgtcg cctgaactac cagactgtat ccgcaactca
     1261 ttgttgtgag tgtggcgatc cgatagatga acgaagacgc ctggcagttc agggttgtcg
     1321 gacttgtgca agttgccagg aggatctgga gcttatcagt aaacagagag gttcgaagtg
     1381 agcgaaatta attatcaggc actgcgtgaa aaggcagaaa aagcaactaa aggaagctac
     1441 atcgtagggc atacatctgt taaccagcac ggcaatttaa caggagtttt tgtttgccaa
     1501 aaatggaaag gagaacccgg tggcgtgatt gcggaatgtc atgttaactg cctgattgaa
     1561 tcagatgctc aggcttatgc aaacgctgaa ttcatagcag aggctaaccc ggctaccgtg
     1621 ctggaactgc tggatgaacg ggaaagaaac cagcaataca tcaaacgccg cgaccaggag
     1681 aacgagggga ttgcgcttac ggttgggaag ctgcgtgttg agcttgaagc agcaaaatca
     1741 aaactcaacg agcagcgtga atattacgag ggagtaatcg cggatggaag taagcgcata
     1801 gcagaactgg aaaaacaatg cgccgaatgg gagcgaaaag cattaagcaa ctttgaagag
     1861 tgtgctgcga tggctgaacg tatcgaagag atgcagacaa aatctgcacc agattcgttt
     1921 ggcatcatcg gtgaaaatat tcgaacacag gacaatcgaa taacgtcaga tcccatgttt
     1981 tgtgtgtatc aaaagcgcga aatcgctgtt gatgctgatt atgaccatga ccggattgtc
     2041 tgggttgacg aagatggcaa tgaagccaat aaacgccata gtcgtcgtct cgagctactt
     2101 catgaaaact ttcgagagcc accagaaaaa tggcggcgcg ttgctgtgaa agatattgat
     2161 gaattcgtta cctgctgttt caccgaacag ggttgtaaag actacctggc agtcaatggt
     2221 cacaatcttc gcttgccatt tatatatgta aaaagcggtt tcaggaacgc tgaatatatc
     2281 ggcataagaa actggcttgc tggcattcgc atcaaaggag agtgatatgg cgttaacaca
     2341 ccgcgaactc tgtcagattg cgtacaagtt ccttaagcgc aacggtttca aggtttgttt
     2401 tcatgaccgc tttatagctg taaccagtac cggagaacag ccagatgcta tgggattcag
     2461 aaattcagca tcatgcctga ttggcgaaat gttctcgtgc tgacttgttg gcagatagaa
     2521 aaaagcgttt tcgtaaaaat ccgtctcttg gaatgggcga ctggcgattc tttattagtg
     2581 agccgggaat tatttcaatt gaggatttac cacctggctg gggattactt cacgttgtta
     2641 acggaagagt acggaaagta catgggtggc ccaagggtaa ttgctgttgg ggtaatcctg
     2701 acgataagcc atttactgga aataagcagg ttgaatgcga ttacatgttg tctgcattaa
     2761 ggcgcatgga gttgagaggg caccttaatg aaatatatga cggtgtaatt gttaataaga
     2821 aagaaggaaa cgcggcatga ccactattac cgacaaagaa ctgattaaag aaatcaaaga
     2881 gcgcataggc agcctggacg ttcgagacaa tattgagcgc cgtgcttatg aaattgcact
     2941 ggcatcgctg gaagcagaac cgatagcgtg ggaatgcggt gaaaacataa tcctgtttaa
     3001 ccctgacaca gttgaagcat acgcaaaacg tgcggaaata tcacctaaac cactattctc
     3061 cgcgccgcca gcgctggtag tgcctgataa gttgccgcgt gaatacagaa acggttggcc
     3121 tcttgcgtat agtgattatg ctgaaggctg gaacgactgc cgcgaagcca tgcttcaggg
     3181 agataaatca tgattaatcg tatcaagctg gagcacatcc tcgaatatgc caggcagcag
     3241 aggcatattg gtcagcattg taaaattcca ccaggagata tggttgaaat catggagatt
     3301 gccatgcgca aggctggcaa ct
//
LOCUS       DBFIFS010000323         3301 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.324, whole genome shotgun sequence.
ACCESSION   DBFIFS010000323 DBFIFS010000000
VERSION     DBFIFS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.324"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            185..979
                     /gene="hyxR"
                     /locus_tag="N7A07_004647"
                     /pseudo
     CDS             185..979
                     /gene="hyxR"
                     /locus_tag="N7A07_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            1133..1894
                     /gene="hyxA"
                     /locus_tag="N7A07_004648"
     CDS             1133..1894
                     /gene="hyxA"
                     /locus_tag="N7A07_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HGU2479150.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1936..3018
                     /locus_tag="N7A07_004649"
                     /pseudo
     CDS             1936..3018
                     /locus_tag="N7A07_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3008..3196
                     /locus_tag="N7A07_004650"
     CDS             3008..3196
                     /locus_tag="N7A07_004650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU2479151.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
BASE COUNT          933 a          694 c          812 g          862 t
ORIGIN      
        1 ccgccaaggt cataccagtg attaactttc actcatgttg ttaaatgcaa ccaaattaaa
       61 aacaataaaa atcaaacaaa ttagatttat attccaagaa aattaaatat tcaggcaatt
      121 aaacaaaacc tgacattttg aatcaaaatg tcaggttttg tagctatggc gtggacgagt
      181 ctgaatgaag aagcctctgg ttattatcag tgcctgtcag tttaccaggc tggcactgga
      241 gtctctcatc ccagcggaca ggtatatcgt gcgggtgtac agcaacgtca ctacagaggt
      301 tgaattcgtt ttaaagacct cctgtggcta tctgttggct gatatccctt agcttccttc
      361 gggggagtta gttcttctgg cttgtcttgt ccgcttgcgg agtgatgccg ggcaatggca
      421 ggtctgcctg accggtgact ccgactggtt cgataatgct cctgctgcaa gtctgtataa
      481 cggcgtccca cgcatcggca ccatgctgac agcgagtcgt tatcagaatc aaattatccg
      541 ctggttacta cgtcctttgc ccggcgatat gagcgatttc tggctcctga ctgcacggga
      601 gttgagcgtg cttaagattc tgatgcaggg catgagtttc agtgagattg cccgttatga
      661 acaacgcagt tcaaagaccc tgcatgcgat tgccacacag gcattaatga aacttggact
      721 gggaactctg agcgatttca ggctgctcta tacgggatgc ggtgacagca gagtgaaacg
      781 gaatatacgg ctacatgcaa gatatgtcgg acaacattcc gctcatcgac tcactcagta
      841 tgtgcagaac atggtatccg gaattcttta tccagagaaa gttgccgttg gtgaatcaat
      901 aaattctgac gccatttccg gaaaagtact tcccggacaa agaaaaaata tttctaaaaa
      961 aagaccagga ttgttatgac acaacagcgt tgttactacc ccccactgcg gcgcgtacgg
     1021 actcgctaca gtgtaggggc caaaatattt ctactgctct gggctgcagg attgggaggc
     1081 acgctgtaca gcctgtacag cgtgctggcg tactgacatc tgtgagggaa ggatgcgttt
     1141 cgttcttttc tgtccctcag gaattgtacc agcgcagttc gcagctctat caaccggttc
     1201 tgtcggcaat gttacctgca tcggaacaga agaggagtta cgcaataaat tgcggcacag
     1261 gccacagagt gtcgtgatat cggcagggcg acctgccgaa tgcgctgaaa tgtggttccg
     1321 attttatcgg gatcactcat tcgtggtggt gttgtgcgtc gcgccatttt ttctgccacc
     1381 ggatgtgagt atttctggcg ttctcaaaaa cctgaggtta ctgaaaccag gaatgtcagt
     1441 agagcatgtc atcagtattg caaattcctc tggtgggtta tcagggctga aacatgcaga
     1501 aaatttgcca gttatggaca gttactctgt ctttatgaaa gaggtgaata accgcacaaa
     1561 gacaatcgtc atgtcagaac gcttcccgga aaagcaaaag aaggtgttgt cgttgcttct
     1621 tgccggacac agctgggaat acagtgcgca gtttctcaaa acggggataa gacagatatg
     1681 gcttgccgag cagtcgctaa aaaagcgttg gggaataccg gacagcatgt cattgcggga
     1741 agccctgctg ctgagcagca ataaatttca cggtgatgga aatgcattgg aaaaaacgaa
     1801 tgccatgaca ttgcgtgaaa acggaaatac caacaggtat tccgtggtgg taaatgcagg
     1861 gacgcaggtt ttatctctgc ataaatataa ataagtggga aattgaaatt agttaccaga
     1921 atggagaatt tcttcatgaa aaacagtaag gcattttacc gcagcgcatt agcgacagct
     1981 attgttatgg ctctttctgc accagcattc gctgctgata acgcggtatc aactgattcg
     2041 gttacgctga ataaagataa gacgactctg gatcaagatg ttgttattag caatacagca
     2101 gacaaacaga ttacagccgt aacaattaat gcggcagatg aagaccttaa tgttgctttt
     2161 gctggtcatg atattactgc tgaatcaacg gcagacaaaa aattcgttga aggtgtaaaa
     2221 gttagtggta acaaaaatgt tgtgattaat gctacagact ccaccatcac agctcaaggt
     2281 gaaggcacct atgtccggac tgcaatggtc attgattcaa ctggcgatgt tgttgttaat
     2341 ggcggtaatt tcgttgcaaa aaatgaaaaa ggtagtgcga cagggatatc tctggaaggg
     2401 gccaagggaa ataatgtcac gcttaatggc acaaccataa atgctcaagg taataagagt
     2461 tccagcaacg cctccacggc aatttttgct caaaagggta gcttattgaa tagttttgaa
     2521 ggtgatgcaa ccgacaacat tacccttgct ggctcaaata ttattaatgg tcagattgaa
     2581 acaatagtta ctgccgggaa taagacggga attcatacag tcaacctgaa tattaaggat
     2641 ggctcagtaa ttggggcggc taataataaa caaacaattt atgcctctgc ttcggcacaa
     2701 ggcacaggtt cagcaacgca aaatttaaat ttgtctgttg ctgattcaac catctactct
     2761 gatgtcctgg ccctttctga aagcgagaat tcagccgcta ccacaacaaa tgtaaacatg
     2821 aacgttgccc gctcttactg ggaaggtaat gcttatactt tcaatagcgg cgataaagcg
     2881 ggtagtaatc tggatataaa tctttccgat agttcagtct ggaaaggcaa agtttcaggg
     2941 gcaggaaatg ccagtgtatc tctgcaaaac gagtctgtct ggaatgttac gggatcctca
     3001 actgttgatg ctctggctcc gtctgggtga tgagtctgtc aatgccagca ttgacggtgc
     3061 agcattccgc gtgggtgcag gtgtacaggc tgatatcacc aaaaacatgg gagcatatgc
     3121 aagccttgac tacaccaaag gtgacgacat tgagaatccg ctacagggtg tagttggtat
     3181 caatgtgacc tggtaagaat aaagcagtcg gttcccctgt cgactgttat accccctgca
     3241 ggcagcagta tgctgcctgc aggctataaa tatccagtat tcatgatgct ggctttttgc
     3301 t
//
LOCUS       DBFIFS010000324         3265 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.325, whole genome shotgun sequence.
ACCESSION   DBFIFS010000324 DBFIFS010000000
VERSION     DBFIFS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.325"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(600..797)
                     /locus_tag="N7A07_004651"
     CDS             complement(600..797)
                     /locus_tag="N7A07_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2479152.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(863..1133)
                     /locus_tag="N7A07_004652"
                     /pseudo
     CDS             complement(863..1133)
                     /locus_tag="N7A07_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1438..1626
                     /locus_tag="N7A07_004653"
     CDS             1438..1626
                     /locus_tag="N7A07_004653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479153.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1954..2148
                     /locus_tag="N7A07_004654"
     CDS             1954..2148
                     /locus_tag="N7A07_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479154.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2491..2619
                     /locus_tag="N7A07_004655"
     CDS             2491..2619
                     /locus_tag="N7A07_004655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479155.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2929..3264)
                     /locus_tag="N7A07_004656"
     CDS             complement(2929..3264)
                     /locus_tag="N7A07_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479156.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          962 a          751 c          719 g          833 t
ORIGIN      
        1 agtttgtagg agctttcttt tttgaacaat gatttgcagg acagttatgc taaactagcg
       61 gaatcagtat cacaacaata aatgactgat catacctgaa atttgaatta acaggcatca
      121 tgaatgtcaa tagtctaagc ggtgactact tatatcgctt gcataaatac taaacactga
      181 ctagaaaagt attggataac cccgtcacta aaagtaacgc ttcggttatt tgctcggaat
      241 gtattaacgc caagattgtt agccgttacg ccttatatca ctggcgctga caaatgtttc
      301 ttttctaatt tactgaaccg gcgcagacac gcaggaaagc acgccggcac cggtgtaatg
      361 ccagttctaa tcaataagaa tggaatgacg aatcagtcag gaagggataa caacccgctg
      421 ctgaacggaa ggtatcagca ggaagatgaa cgggaatatc gccagcacca agggcagggc
      481 cgggttccac aataccagcg ctggcatttt gcacattgcc agcgtaccgg cattacaaca
      541 cgtttcatgc tgctgccccc caggattcct cgattcgttg ctacatccac tgctctactt
      601 cacttccact gctccacttc acttttgtac cagcggctgc tgcgccccag tttgatgggt
      661 ttagggaaat ggccttcact gatcaacttg taaaaccatt tgtcggtcat accggtgaaa
      721 ctggtgatat agttcatgtc gatcagggaa ttatcggcac gcagaaatac gctgtcgtgg
      781 cttgttgtag tcaacatggt cagaatgtcc tgtgagattt atgaagattg tgcgaatgcg
      841 ggaaatctac tgagctgtgc tttcagaact ggcctgttac gggataccag ggattaccgg
      901 cggggtaacg ggcttccgga tcatacacac cacgattatc gcggacaaaa tcactgaacg
      961 cccatatcac ctctttaagt atgtcttcgc agtccggtaa atgacgatcc agcgccacat
     1021 cccgggtggt acagctttga tgcgcccggt gacacaaagc ccggattgtt ccagacatcc
     1081 tgaatcaaac gccccagatt aggggcgtcg aaatatgcct ctctgaccat tatattccgg
     1141 cgtataggta gcaggtctga agtgacaatg cgtcacctga cgttaaaagt cacccaagat
     1201 gaccccagtg ggggcaacaa gtataaaacc gttttttgat taagtggtcg gatatgaaaa
     1261 ggggaaggtt tatgtgaaaa atacctgtgt tttctttgaa aacacacaac agtatcaggt
     1321 tatttatgtt tatacagtgc gttccaaact gacctgaata attacgcaag ttgcaacaat
     1381 aaaacatttt tgccgtagtg tgaggtactc cggtaaattg cttcccaggt gcagacaatg
     1441 actaacgaag aactacaggc aatcgcatta tccatgcttg agcgccagcg cgaaaaagta
     1501 caccagcaac gagagtcttt gcttaaccca tctccattca ccaggccaaa acgattggag
     1561 cttgaagcat tcttggacaa atacccacgg cggcttaaat caggcaaaac cagaccacca
     1621 ggctgatagc tcacagtttg ttctccgatt caataaaacg gataagaacc atcagcgcgg
     1681 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
     1741 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
     1801 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
     1861 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
     1921 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
     1981 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
     2041 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
     2101 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
     2161 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatatcca
     2221 ctgacgagtt tgccggtcat tctggtatca tgcagatatt tacggcgtac aagcacacta
     2281 gcatgacatt gtaataaggc ctgagcctaa cgctttaatg tgtgtcatac actcagtggc
     2341 gtaatcgttg acaatatggt gcatgtaatc gtaagcgtcc agcgaaatcc atctggtcat
     2401 aatctgaagc atccgacaaa gtggtgtcca ccaaataagt gatctgcgcc caatatcctg
     2461 agccggaact ttgacaaagg ccaacgaaaa atgggagcgg atgttacaga gttctcggta
     2521 caggggaaaa gtggtacctg tcgccagttc tccgatctgt ttaaccggga aattatctcc
     2581 tacagcttgt cagaaaggtc ggtgatggag catcgttaat acgatgctat acgatgcatt
     2641 cacagtgccc ggccagagga tgccccgtcg ctgcatatgg atcagggttg gcaatatcga
     2701 attgcaggct ataggcaaag ttaaagcccc atggagtagc acaaaatatg ccgcacaaag
     2761 gaaacggtct gaataacgca gtgatgaaga acttcttcag cacactgcta aaacgcatag
     2821 tgatcgagcg ctgattctgg cgaacaactg aactaataca tcagaatctg cattatgtta
     2881 aataaatata aaaagatggt ttaaataccc cgttactttt gacttacact atacggtatc
     2941 gcatcgttta atattcgcac cggccagatt tttatttcta ttagttgtca caatactgaa
     3001 tgcgtacgac cacagtattc tggctcctgt gtggttatgc tttaattctg cgttccgggc
     3061 agataagcag ttgcttgcag gaatccttct tgtgttaatg tcagttcccc ttttaccagt
     3121 gctgatttcc acattccgtc caacagagct tatagccttt ccctgtatta tagcattgtc
     3181 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
     3241 ataacccact gtactgataa ccata
//
LOCUS       DBFIFS010000325         3264 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.326, whole genome shotgun sequence.
ACCESSION   DBFIFS010000325 DBFIFS010000000
VERSION     DBFIFS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.326"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(107..733)
                     /gene="dsbA"
                     /locus_tag="N7A07_004657"
     CDS             complement(107..733)
                     /gene="dsbA"
                     /locus_tag="N7A07_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU2479157.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(750..1736)
                     /gene="srkA"
                     /locus_tag="N7A07_004658"
     CDS             complement(750..1736)
                     /gene="srkA"
                     /locus_tag="N7A07_004658"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU2479158.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1813..2082)
                     /locus_tag="N7A07_004659"
     CDS             complement(1813..2082)
                     /locus_tag="N7A07_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU2479159.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2152..2736
                     /gene="mobA"
                     /locus_tag="N7A07_004660"
     CDS             2152..2736
                     /gene="mobA"
                     /locus_tag="N7A07_004660"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU2479160.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2718..3245
                     /gene="mobB"
                     /locus_tag="N7A07_004661"
     CDS             2718..3245
                     /gene="mobB"
                     /locus_tag="N7A07_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU2479161.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          865 a          781 c          780 g          838 t
ORIGIN      
        1 tgaaataatg tccagcggca ggatgcatta tcagaatgta tttatattta ttaaataata
       61 aaaaaagccc gtgaatattc acgggcttta tgtaatttac attgaattat tttttctcgg
      121 acagatattt cactgtatca gcatactgct gaacaaaaac atccatattg ctggtatcca
      181 taccctgcgg attcagctga tatttaccgt taacaaacat cgccggaaca ccacgcaatt
      241 gcacgtcagc tgcagctttt tcctgctgag cgaccagaga tttcaccacg aagctgttcc
      301 acgccgcgtc gtactcttca cctttaatac ctgcgttgat aaatacatcg cggatatcag
      361 atgctgaacg aatggtctgg gttttctgta cgccttcaaa cagcggaaca gtcactttgt
      421 cttccacgcc cagcgccatc gccacagccc atgcctgagt cagatctttg cccaggtcac
      481 cacccatgaa gttgacgtgg tatttagtca tcttcacgcc ttccggcagt tttttcttca
      541 cattatcaga aatatgcaga acttcttcaa actgatagca gtgcgggcag aagaaagaga
      601 aaaactccag cacttgcggc gcgccagcta ccggtttttc cagggtagtg tactgtttac
      661 catcttcata ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc agcgccagcc
      721 aaatcttttt catgatctac tctctccgat taatacatag gtgttaattg caaagggggt
      781 tcttgtagaa cttttgcctg ttctataaaa gtcgccgtct gtcgtagcca gtaatcttcc
      841 ccggttaacc acgggaaatt tttcgggaac gcgggatcag cccaacgccg cattagccag
      901 gcaagataat aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat ttcagcggtg
      961 tcgaactcgc taaattcttc ataagcttca ataatagttt ccagttgcat ccgctgctcg
     1021 gctttatcgc cattgagcaa catccacaaa tcctgaatag ctggaccatt acgtgcatca
     1081 tccagatcaa caaacattgg accatcgcgc cagagaatat tcccggcgtg gcagtctcca
     1141 tgtagccgca gaacggtgaa atcttcccgc cagtgtgctg taacggcggc gatcagctca
     1201 tctgtcgctt tcaggaatgc cgctttcaac ccggaaggta tcagtgtagc gtcctcaaac
     1261 agcttgcgtg gctcaatgag atattcattc agaccgatgg tcgggcgatg gataaaaagc
     1321 tgtttgcgcc ccgtctggtg catacgccct aaataacgcc caaccgcttc catctgatcg
     1381 atattatcag cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa ataaaatccc
     1441 tgatgattca ataaagtctg accgttaaaa gccacaggtg ctgcaaccgg aacttcatca
     1501 tttaccagct gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt ccaacgttca
     1561 ggacgataaa atttgacgac aaaacgtcga cgatcttcgt cctgaaattg atagacacgg
     1621 ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc ggatcccttg ctcaaacaga
     1681 gcgtccatga tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt gttcatccca
     1741 tcatccggaa aacattacga atgatgaagg atatcatttc acgatgattt tggtggcgcc
     1801 gcttacaagc ttttactctt taattacgcc gcgagcgcgt aacagcgccg ttttgaagtc
     1861 ttcttcataa tctttctgca aacccgggat caccgcatct ttcgcggaat cacgcatttt
     1921 caggtgatag atcagaatgt catccgtcaa atccgccagt tcgccgtcaa aacctgactc
     1981 tttcgccagt ttctgcaaaa attgcaacag gttaaggtct ggctcttttt gccaggctgg
     2041 ctggaggagt tcaataactt cattcagacg tttacatttc atagtaatgc tccttactct
     2101 tgagacagac acgttagcag ggtcaatccc acaataaaag aggcgatatc ggtgaatctg
     2161 atgacgacga taacaggcgt tgtgctggca ggcggtaaag ccagacgaat gggcggcgta
     2221 gataaaggat tgctggactt aaatggcaaa ccgctgtggc aacatgtcgc tgacgcgctt
     2281 atgacgcagc tctctcacgt cgtggttaat gctaatcgtc atcaggaaat ctatcaggca
     2341 ggcggtctga aagtgattga agattcactg gcggattacc caggccctct ggcagggatg
     2401 ctttcagtaa tgcagcagga agctggtgag tggtttttgt tttgtccgtg cgatacgcct
     2461 tacattcccc atgatttagc cgcccggctt aatcatcagc gcaaagatgc gcctgtcgtg
     2521 tgggtccatg acggtgaacg cgatcacccg actattgcta tggtaaaccg cgctattgag
     2581 cctttattac tagaatatct gcaagcagga gaacgccggg taatggcatt tatgcgtctg
     2641 gctggcggtc atgcggttga tttcagcgat cataaagatg catttgttaa cgtgaatacg
     2701 ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc tttgccgcgt
     2761 ggagtggtac cggaaaaact acgcttttga aaaaactgat cccggcatta tgcgccagag
     2821 ggatccgtcc agggctgatt aagcatacgc accatgatat ggatgttgat aagccaggca
     2881 aagatagcta tgagctgcgc aaggctggcg cggcacaaac catcgttgcc agccagcaac
     2941 gatgggcctt gatgacggaa acaccagacg aagaagagct ggatctacag tttctcgcaa
     3001 gtcgaatgga tacctcaaag ctggatttga ttctggtcga agggtttaag catgaagaga
     3061 tcgcaaagat tgtgctgttt cgcgatggag ccggacatcg acctgaagaa ttagtgatag
     3121 acaggcatgt tattgctgta gccagtgatg tgccgcttaa tcttgatgtc gcattactgg
     3181 atattaatga tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa aatcaggacg
     3241 gataagcaag agtaggctgg agca
//
LOCUS       DBFIFS010000326         3251 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.327, whole genome shotgun sequence.
ACCESSION   DBFIFS010000326 DBFIFS010000000
VERSION     DBFIFS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.327"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(67..756)
                     /locus_tag="N7A07_004662"
     CDS             complement(67..756)
                     /locus_tag="N7A07_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU2479162.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQPTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            861..1091
                     /locus_tag="N7A07_004663"
     CDS             861..1091
                     /locus_tag="N7A07_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2479163.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAHQIRPDLPKLFPHTEKNAA"
     gene            1161..1700
                     /locus_tag="N7A07_004664"
     CDS             1161..1700
                     /locus_tag="N7A07_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HGU2479164.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1697..2716
                     /locus_tag="N7A07_004665"
     CDS             1697..2716
                     /locus_tag="N7A07_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2479165.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNALIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGMIASKPKLDLTNTDWI
                     YGVDL"
     gene            2713..>3251
                     /locus_tag="N7A07_004666"
     CDS             2713..>3251
                     /locus_tag="N7A07_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2479166.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAA"
BASE COUNT          913 a          778 c          790 g          770 t
ORIGIN      
        1 taaaatgaat aattaagttg ttttactgaa tgagcacaat gcccggttga taatttttaa
       61 ttggtactat ccatgcttcc tatacgtctg cggcatgctc ccaataactt tcccgaagat
      121 aaacacccgg ttcatctcgt ctttctcgat tgggtcccac ggtgagtagc tcttgttatc
      181 agagataacc agcagtttat ccttcatcat ttgcaggcgc tttacatggg ctgtgtcgtc
      241 gtacagaaac gcatagatac catcaccgtc gaaagattta accgtgatat caacgaacag
      301 cagatcacca ggttcaatcg ttcctgacat gctgtcaccg cgtacgttaa tgatgcggat
      361 attttctgcc ttcctgccat cgaacatgtg acgagcatcg tcaaacgagt actcaaccga
      421 gcgtaggact tctacaaact cacggttgat gacacccggc ccggcactca cttctatatc
      481 aagaacgtca atcttaaagt atttggaatg gctgacagtt ggttgtattg gttgcactgt
      541 actgtctgac atatttccaa cgccagaaga taaccattct gcgcgcacac ccaaagcgtt
      601 cgcgatctcc acgattttag ttgtttgatt agctttccct gtttcgattt tctgaatagc
      661 agcttggcta accccgacca aatccccaag cgccttttgt gtaaggcctc gcgctaatct
      721 ggcttcttta agtctttctg agagtgttgt tttcatagtt caaatgtaca accaaggttt
      781 tattccatca aacgaaaatg gttgttgact aaaaacaacc atagttttaa tcttgattca
      841 aattaaccac ggaggttgtt atgaacccag ctattaaaac agcgatcaat atcgttggtt
      901 cacaaaagaa actgggcgct gcttgcgaag tttcacagca ggccgtctat aagtggcttc
      961 acaacaaagc aaaggtatcc cctgaacatg tcggcagcat tgttacggct actggtggag
     1021 tagtgaaggc acaccagatt cgcccggatc ttccgaagtt gtttccacac accgaaaaga
     1081 acgcagctta aatttccatt tcacgctctt taacaataag caatcaactt aacagtcaat
     1141 tcaaactaaa ggagtcaatt atgcaaccac ttacatacca acagactagc ggatttagcc
     1201 cgactgcggt gataaatcgt tctcaaacaa aacaggtgcc aggccacgaa aaaatccgtg
     1261 atgccgtccg cgcctggtcg gctgaagata atcaggatgt cgttgccaca ctcattgtga
     1321 atgagtatcg ggagcagggc ggcggcacca tcgatttccc tgatgatgtc agccgtgcac
     1381 gccagaagct gttccgcttc ctcgataaca aattcgattc tgaaaaatac cgaaataacg
     1441 tgcgtgaact gaccccggca attctggcgg tactaccgct ggaatatcgc ggttacctgg
     1501 ttgagcagga tagctttatg gctaggttgg ctgaaatgga aaaggaactc agtgaggcaa
     1561 aacaggctgt cattctcaac gcaccacgcc accagaaact gaaggaaatt agtgaaggta
     1621 ttgtgtcgat gtttcgtgtg gacccagatc tggctggtcc actgatggcg atggtcacca
     1681 ccatgctggg ggcaatatga caggttcaga aatggcgaaa gccggtctgc tggaacagaa
     1741 ccgactttca ggtgcaaatc gtaacgcact cattgcggga ggaattatgg caaacactgc
     1801 tgagatattc aattttccag tgccggatgt ggcacaaaag gagccgcgcg tggcagatct
     1861 cgatgatggt tatacgcgca ttgcaaatga gttgctggaa gctgtgatgc tggccggatt
     1921 aacacagcac cagcttctgg tcttcctggc tgtcatgcgc aaaacatatg gctttaataa
     1981 aaaactggat tgggtgagca acgagcaact ttccgaattg accgggatat tgccgcacaa
     2041 gtgttctgct gcaaaaagcg ttctggtaaa gcgtgggatt cttattcaga gcgggcggaa
     2101 taccggcatt aataatgtgg tcagtgaatg gtcaacatta cccgaatcag gtaagaaaaa
     2161 taaagtttac ctgaaagagg taaatttacc tgaatcaggt aaaaaaagtt tacccaaatc
     2221 aggtaaaggc gtttacccga atcaggtaaa cacaaaagac aaactaacaa aagacaatat
     2281 aaaacctttt tcgtccgaga attctggcga atcctctgac caaccagaaa acgatcttcc
     2341 tgtggtgaaa ccggatgctg caattcagag cggcagcaag tgggggacag cagaagacct
     2401 gaccgccgca gagtggatgt ttgacatggt gaagaccatc gcgccatcag ccagaaaacc
     2461 gaattttgca gggtgggcta acgatatccg cctgatgcgt gaacgtgacg gacgtaacca
     2521 ccgcgacatg tgcgtgctgt tccgctgggc atgccaggac aacttctggt ccggtaacgt
     2581 gctaagtccg gccaaactcc gcgacaagtg gacccagctc gaaatcaacc gtaacaagca
     2641 acaggcaggc atgatagcca gcaaaccaaa actcgacctg acaaacactg actggattta
     2701 cggggtggat ttatgaaaaa catcgccgca cagatggtta actttgaccg tgagcagatg
     2761 cgtcggatcg ccaacaacat gccggaacag tacgacgaaa agccgcaggt acagcaggta
     2821 gcgcagatca tcaacggtgt gttcagccag ttactggcaa ctttcccggc gagtctggct
     2881 aaccgtgacc agaacgaact gaacgaaatc cgccgccagt gggttctggc tttccgggaa
     2941 aacgggatca ccacaatgga acaggttaac gcaggaatgc gcgtagcccg tcggcagaat
     3001 cgaccatttc tgccatcacc cgggcagttt gttgcatggt gccgggaaga agcatccgtt
     3061 atcgccggac tgccaaacgt cagcgagctg gttgatatgg tttacgagta ttgccggaag
     3121 cgtggcctgt atccggatgc agagtcttat ccgtggaaat cgaacgcgca ttactggttg
     3181 gttaccaact tgtaccagaa catgcgggcc aatgcgctga ctgacgcgga attacggcgc
     3241 aaggctgccg a
//
LOCUS       DBFIFS010000327         3235 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.328, whole genome shotgun sequence.
ACCESSION   DBFIFS010000327 DBFIFS010000000
VERSION     DBFIFS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.328"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..746
                     /locus_tag="N7A07_004667"
     CDS             <1..746
                     /locus_tag="N7A07_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2479167.1"
                     /translation="SETVIHRYGTGAMVLCWHCDNQLREQTSDSLDQLAQQNLAAWMI
                     DIIRHAMNGAQERELSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYRESDI
                     IPGEQTATSILKQRTKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFRKRPKRRRW
                     VNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHAD
                     PLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            759..992
                     /locus_tag="N7A07_004668"
                     /pseudo
     CDS             759..992
                     /locus_tag="N7A07_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            988..2289
                     /locus_tag="N7A07_004669"
                     /pseudo
     CDS             988..2289
                     /locus_tag="N7A07_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
     gene            2437..2619
                     /locus_tag="N7A07_004670"
     CDS             2437..2619
                     /locus_tag="N7A07_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2479168.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2657..2926
                     /locus_tag="N7A07_004671"
     CDS             2657..2926
                     /locus_tag="N7A07_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2479169.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            3002..3217
                     /locus_tag="N7A07_004672"
     CDS             3002..3217
                     /locus_tag="N7A07_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2479170.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          826 a          759 c          947 g          703 t
ORIGIN      
        1 atagcgaaac cgtcattcac cgttacggta ccggcgcgat ggtgttgtgc tggcactgcg
       61 acaaccagct gcgggagcag acatctgatt cactggatca acttgctcaa cagaatctgg
      121 ccgcctggat gattgacatc atccgtcacg caatgaatgg cgcacaggag cgtgaattat
      181 ctctggctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
      241 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctccgta taccgtgaaa
      301 gcgacatcat accgggagaa cagaccgcca ccagcatact gaagcagcgc acaaaaaata
      361 ttgcgctacc gtctcacacc caccagcaac agaacccacc acaggaaaag acggtggtca
      421 gcattgccgt tgatccggag tctccggaat ccttcaggaa acgacctaaa cgtcgccgct
      481 gggtaaatga gaaatacaca cgctgggtaa agacacagcc gtgtgcgtgt tgtggtaagc
      541 cagcggacga tcctcatcat ctgattggtc atggtcaggg cggaatggga acaaaatccc
      601 acgatatttt cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat gcggatccgc
      661 tggcgttcga agaaaagcat ggttcccagg ttgatttaat ttttcgtttt cttgatcacg
      721 cctttgcaac cggcgtgctc gggtaaaaga ggttactgat gcgtatagag tttgttttgc
      781 tttacccgcc gacggtgaac acctactggc gacgtcgtgg cagcacatat tttgtatcaa
      841 aagccggtga gcgttatcgc cgggctgtgg cgcttattgt tcgccagcag cggctgaaat
      901 taagcctgtc cggaaggctg gcgataaaga ttattgccga gccaccggat aagcgccgcc
      961 gtgacctgga caatattctg aaagcgccgc tggggtgtgg gcaggccgct gtataaggat
     1021 ctcatcggtc gtacaaaagc cgcgctggca aagaacccga aaaatgtgct gcttgccgtg
     1081 gtgtggatgc agggggaatt tgactttgac ggaacgccag caaatcacac agcccgtttt
     1141 acagaagtag tggaacaata tcgtacggac cttgcagata tggtgggaca gtgcgctggt
     1201 ggttctgctg acggtgttcc ctggatatgt ggagacacaa cttatttctg gaagcagaag
     1261 agcgaatcca cttaccagac ggtgtacggc agttacaaaa acaaaacgga aaagaatatt
     1321 cactttgtgc cgttcatgac cgatgagaac ggagcaaatg tcccgacgaa caaaccggaa
     1381 gaagacccgg atattccggc atcaggatat tacggtgcgg cctcccggac gtcggcaaac
     1441 tggacgtcag cagaccgtgc gagccatttc agctcatggg cacgcagggg gattatttct
     1501 gaccgtcttg cctcagcgat tcttctccat gcaggacgga cggctgaact ggtgggtggg
     1561 gaacaggttg tgatgccgcc ggatgagaag ccgtcaccgg acacaccatc aacaccgtca
     1621 acggacggga aatcagtgac aacgctgctt tattaccgtg caacagagtc aggtggttta
     1681 ctgaatccgc agggatgggg agctgaagga gggcgtgcat tggtagttga tgatgcaggt
     1741 gctgcaggag gtaaggcgct gaggtggacc aaacagacag gaagttcctc gtggtttatg
     1801 cagcatgatg ccggtaatgg cgcagacctg ctggagaagg gcgggcttat cagttgtcgt
     1861 tttaaagttg atggcacact gacagctaat cagtacgcac tggcgctgta ctggccggtt
     1921 tcttcactgc ctcagggtgt cacactggaa ggtaatgccg gtcataacct gctggcgtcg
     1981 ttttacgtac agagcgatgc cacagacctt aatgtgatgt accacaaggg aaatgctggt
     2041 cagaacacga agctggggtc attcggcgca tttgataacg aatggcatac gctgggcttc
     2101 cgttttgccg gtaacaacag tattgaggtg acgccggtca ttgatggtaa ggacgggacg
     2161 ccgttcatgc tgtcacagtc accggtcggc acgtttacgg cagacaaatt gcgcgtgacc
     2221 gatatcacta gcggtgcgac atatccggtg ctgattgaaa gtataacagt ggaagtgaat
     2281 aacccgtaag caggaaaaaa aggccgccgg ggcagggaaa acaaggagcc agaaccggcg
     2341 gcaaatgtcg ttatatccaa agcaaaacat gcaggacact tttttaacca acaggtatta
     2401 acgatgtcaa caccatatca ataaccggga gggataatga gatttgtaca gcttatttta
     2461 ttgtatttct gcacggtggt gtgcacgtta tatctggtaa gtggcgggta taaggttatc
     2521 cggaactata tacgcaaaaa gattgatgcc gcggcggcgg aaaaaatcag cgccagccag
     2581 tcagccggaa caaaacccga agagcctctc atttcgtagc aactttctta acaacacctt
     2641 tcaacgagaa aatcccatgt cagaaataaa atctctggtc actgctgaag cagtgaagga
     2701 cgtcctgcgc tctgaagaag tcagaagcgc actgaaacag caactccgcc agaatcttga
     2761 ggcgcgtctt gatgctgaag tggatgccat tctggatgag ctgctggggg gaccggctgc
     2821 tcctgagcct gaagacggcg cgggtgacag tgctgtttca gatggcgttg tgtctcagcc
     2881 tgacggtagc agtgagcctc agcctggcgg cgaaatgatg atgtaaccat acgcaggggc
     2941 tgtcggtgtg agctgatgcc cctcctgttg ttgtgagctt ccggattgcg ggagacgggg
     3001 tatgtaccag atggaaaaaa tcacaacggg tgtgtcatac accacgtcag cggtggggac
     3061 gggatactgg ttactgcagc tgctggacaa agtctctccg tcccagtggg tggcgatagg
     3121 tgtgctgggg agtctgctgt ttggcctgct gacgtatctg actaacctgt atttcaaaat
     3181 cagagaggac cgtcgtaagg cggcgcgggg agagtaaagc gatgaagaaa aaata
//
LOCUS       DBFIFS010000328         3232 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.329, whole genome shotgun sequence.
ACCESSION   DBFIFS010000328 DBFIFS010000000
VERSION     DBFIFS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.329"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(62..823)
                     /gene="kduD"
                     /locus_tag="N7A07_004673"
     CDS             complement(62..823)
                     /gene="kduD"
                     /locus_tag="N7A07_004673"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU2479171.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(853..1689)
                     /gene="kduI"
                     /locus_tag="N7A07_004674"
     CDS             complement(853..1689)
                     /gene="kduI"
                     /locus_tag="N7A07_004674"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU2479172.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1976..3157)
                     /gene="yqeF"
                     /locus_tag="N7A07_004675"
     CDS             complement(1976..3157)
                     /gene="yqeF"
                     /locus_tag="N7A07_004675"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU2479173.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          855 a          885 c          723 g          769 t
ORIGIN      
        1 atgatctaga gatataaaaa accctgccat gcggcagggt cataaaagta agaggaatga
       61 attaacgcgc cagccaaccg ccatccacag caatggtata accatttaca taatctgaag
      121 cgctggaggc aagaaacact accggcccca tcaggtcact cggcagtccc caacggccag
      181 ctggaatgcg gtcgagaatt tccgcgctac gttgttcatc tgcccgcagt tgttgggtat
      241 tgttggtcgc catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt
      301 cattcgccat caatcgcgtc acgcccatca cgccgctttt tgatgcggta taagaaggca
      361 cacggatccc gccctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc
      421 cttgcgcgat gaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca
      481 ggttcatgac atcgtcccag tccttttcgc tgaactcgag agcatcttcg cggcgaatca
      541 atccggcgtt attcaccagg atatcaatat gaccaaactc agctaccgcg cgatccagca
      601 gtccaggaat accatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca
      661 gcgccgtgac ctgcttgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac
      721 agcccgcttg cgccagcccc aacgccatcc cctggcccag cccagtatca caaccagtga
      781 cgaccgcaac tttaccttcg agagaaaatg cacttaaaat cataacaata ccttattcgt
      841 ttatgcccac aactagcgca aatctttaac ggccacatgg tccatatcat caaagacctg
      901 gttttcaccg accatgcccc agataaaggt ataagctttg gttccgacgc cggaatgaat
      961 cgaccagctc ggggagatca ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg
     1021 cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac
     1081 ttccatccgg cgctcgtggg tgtgacacgg catggtgttc cacaagttac ccggagccag
     1141 ctccgtcagc cccatactca attggcaggt ttccagcaca tccgggacaa aatatttgtt
     1201 aatcgtgcga cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg
     1261 tgtgactttt ttggtaggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc
     1321 cggagtgccg gtatcaatac tagcaaagac aacttctttt gcgcctttac ccacatacag
     1381 ggcgtcgcgg tgaccgattt catagcattg accatcgaca gtaatcgtac cggcaccgcc
     1441 aatattgata acacctaact cgcgacgttc aaggaaataa cttacgccga gttgtttacc
     1501 cacttccccg ccaacggaaa ccgttttagt taccggcata atgccgccaa taataatacg
     1561 gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa
     1621 ttcattgcgc agcccctggg tttccagcgt ttttgcgtgc gcactgtgga tgctctgtct
     1681 tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac
     1741 actgtttcgt tttctctata aggggatgat atgcccatgg gatcgggttt tcaattaaaa
     1801 ataaaacaat gtttcatttt taagttaagg attaaaaaaa gtgcagtgtc gatcacgaat
     1861 ctgtcatgag aatggaaaaa tcagggagcc cggaaaatgt atgtcgactt gccagccatc
     1921 attattcaac agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctatt
     1981 cgtcacgttc aatggtcaat gccacaccct ggcccccgcc gatacaaagc gttgccagtc
     2041 ctttgcgggc attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg
     2101 aagcgcctat cgggtgaccg agtgcgatcg cgccaccatt gacattgacc cgacgctcat
     2161 cccactcaag catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga
     2221 taagatcaac atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca
     2281 ccggcgcaat tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg
     2341 ccagcacggg taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg
     2401 cgccatcgtt tatggatgat gcattacccg ctgtcacaga accgagacta tcaaatgaag
     2461 gatttaaacg ggctaaacct tctgcactgg cgtcagtgtg tggctgttca tcggtatcaa
     2521 caaccaacgt ctgaccgtta ctttgggtca ttaccgggac gatctcatct ttaaatcgtc
     2581 cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaatt
     2641 gacggctgat gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat
     2701 tgaaggcatc ccataaccca tcatgcacaa gactgtcaac cagctggcta ttgccaagct
     2761 gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac
     2821 cggcgatgac aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt
     2881 taagcccgga accgcagacg tcattaatcg tgattgcaga aacgctatta ggcagaccac
     2941 ctttaatagc cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa
     3001 gaattacttc atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga
     3061 ccagactacc aagttccacg gcggaatgac ccgctaacgc accgcgaaag cagccgatag
     3121 gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag
     3181 tgcaatagta aatgattgtt aattacagtt atcttaattg tttaaaaaaa gt
//
LOCUS       DBFIFS010000329         3229 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.330, whole genome shotgun sequence.
ACCESSION   DBFIFS010000329 DBFIFS010000000
VERSION     DBFIFS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.330"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            78..656
                     /gene="espA"
                     /locus_tag="N7A07_004676"
     CDS             78..656
                     /gene="espA"
                     /locus_tag="N7A07_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU2479174.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            669..1793
                     /gene="espD"
                     /locus_tag="N7A07_004677"
     CDS             669..1793
                     /gene="espD"
                     /locus_tag="N7A07_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HGU2479175.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1814..2752
                     /gene="espB"
                     /locus_tag="N7A07_004678"
     CDS             1814..2752
                     /gene="espB"
                     /locus_tag="N7A07_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU2479176.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2759..3166
                     /gene="cesD2"
                     /locus_tag="N7A07_004679"
     CDS             2759..3166
                     /gene="cesD2"
                     /locus_tag="N7A07_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU2479177.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
BASE COUNT          950 a          619 c          788 g          872 t
ORIGIN      
        1 taaggaggaa attatttgat attaattact caataatttt tttgttttcc tgagaaaaat
       61 tatcaagagg tatatagatg gatacatcaa atgcaacatc cgttgttaat gtgagtgcga
      121 gttcttcgac atcgacgatc tatgacttag gtaatatgtc gaaggatgag gtggttaagc
      181 tatttgagga actcggtgtt tttcaggctg cgattctcat gttttcttat atgtatcagg
      241 cacaaagtaa tctgtcgatt gcaaagtttg ctgatatgaa tgaggcatct aaagcgtcaa
      301 ccacggcaca aaagatggct aatcttgtgg atgccaaaat tgctgatgtt cagagtagca
      361 ctgataagaa tgcgaaagcc aaacttcctc aagacgtgat tgactatata aacgatccac
      421 gtaatgacat aagtgtaact ggtattcgtg atcttagtgg tgatttaagc gctggtgatc
      481 tgcaaacagt gaaggcggct atttcagcta aagcgaataa cctgacaacg gtagtgaata
      541 atagccagct cgaaattcag caaatgtcga atacattaaa tctcttaacg agtgcacgtt
      601 ctgatgtgca atctctacaa tatagaacta tttcagcaat atcccttggt aaataaccgg
      661 agataactat gcttaacgta aataacgata ccctgtctgt aacgtctggg gttaataccg
      721 cctcgggtac ttctggtatt actcaatctg aaacgggttt atcgctggat ttacaactgg
      781 ttaaatccat gaactcgtca gcaggctgga cagaaagtag ccctttaccg acgccgccgg
      841 caggtcactc attagtgacg ccctctgctg ctgaggatgt ccttagtaaa ttgtttggtg
      901 gtattagtgg tgaggttaca agtcgcactg aggaggcaga gccacagcgc acaagctatc
      961 cctatctctc tcaggtgaat accgttgacc ctcagcaaat gatgatgatg gtcactctgt
     1021 tatccctgga tacttccgcg cagaaagtct cgagtctgaa aaactctaac gagatttata
     1081 tggatgggca aactaaagcg ctggagaata aaacgcagga gtataaaaaa cagctcgaag
     1141 aacaacagaa agccgaagag aaatcacaaa aaagtaaaat tgttggccag gtctttggtt
     1201 ggttgggcgt cgcattaaca gccgttgccg ctgtttttaa cccagcactc tgggctgttg
     1261 ttgccattgg tgcaacagca atggcactgc aaacggcagt cgatgtaatg ggggaaaatg
     1321 cccctcaggg attaaagact gcagcacagg tctttggcgg aatatctatg gccgcaagca
     1381 ttctgacagc cggcgttggc ggggtgtctt cactgttatc taaatttggt aatgttgcta
     1441 acaaaattgg ctcaagcgtt gtaaaagtcg ttgagaaggc ggcagaagcg ctggttaaaa
     1501 acgtttttgc aaaaatttcg acagtggctg agggcgttac gaacggtatt cgttctgccg
     1561 ggacaactgc gttgaataat gaggctgcgc aactccaaat gttgtctcag ttagctgctt
     1621 tcgcggtgca aaacttaact cgacagagtg aaagcttagg tgagagtgcg aagctcgagc
     1681 tggataaagc ggcaagcgag ttacaaaatc aggcgagcta tttacaaagt gtttctcaac
     1741 tgatgtccga ttcagcacgg gtaaatagtc gtattgttag tggccgaatt taattattaa
     1801 agagaatttt attatgaata ctattgataa tactcaagta acgatggtta attccgcttc
     1861 ggagagtacg accggcgctt ccagtgcagt tgccgcatct gctttatcaa ttgattcatc
     1921 tctgcttact gatggtaagg ttgatatttg taagctgatg ctggaaattc aaaaactcct
     1981 cggcaagatg gtgactctat tgcaggatta ccaacaaaaa caattggcgc aaagctatca
     2041 gattcagcag gccgtttttg agagccagaa taaagctatt gaggaaaaaa aagccgcggc
     2101 aaccgctgct ttggttggcg ggattatttc atcagcattg gggatcttag gttcttttgc
     2161 agcaatgaac aacgcggcta aaggggctgg tgagattgct gaaaaagcaa gctctgcatc
     2221 ttcaaaggct gctggtgcgg cttctgaggt tgcaaataaa gctctggtca aggctacgga
     2281 aagtgttgct gatgtcgcag aggaggcatc cagtgcgatg cagaaagcga tggccacaac
     2341 aacgaaagca gccagccgtg catctggcgt tgcagatgat gttgcgaaag cctctgactt
     2401 tgctgaagat cttgcagacg ccgccgagaa gacaagcaga atcaataagt tgttgaattc
     2461 cgtagataaa ctgaccaata ccacagcatt tgttgccgtg accagtcttg ctgaaggtac
     2521 gaaaacgttg ccaacaacaa tatctgagtc cgtcaaatcg actcatgagg ttaatgaaca
     2581 acgtgcgaag tcgctggaaa acttccagca ggggaatctg gagctgtata aacaagacgt
     2641 tcgcagaacg caggatgata tcacgactcg tctgcgtgat ataacgtccg ctgtccgcga
     2701 tctccttgag gtccagaatc gtatggggca atcgggtcgc ttagctgggt aattgatcat
     2761 ggtcgatacg tttaatgatg aagtgtttaa ttactatctt gaacaaaaag ggtacacaat
     2821 acagaaggag tttctttgtg gcagtgcctt ttttatcgga tggcggattg agacaccttt
     2881 tttttcatta gcgtacagac tggatgaaca agaactgatt ttgtgctctt ttgaagcacg
     2941 taaccaaaca gggcttaacg gccctgtttt atcgctgact catttgctcg aagagttgta
     3001 tcaccatttt tcgggtatta agaaaatcag cgcgatgaaa tctaagattg gttcagattc
     3061 agaacgtcaa aagcgcgaag agttgtttaa ttacttcatc agaaagggtg ccgtgcagca
     3121 agaaacagaa gacggaattt ggttcgtaat gaacgtaaat agttaaaagt ttttatttta
     3181 ttaacttctg agggaaattt aatgaattta tctgaaatta ctcaacaaa
//
LOCUS       DBFIFS010000330         3224 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.331, whole genome shotgun sequence.
ACCESSION   DBFIFS010000330 DBFIFS010000000
VERSION     DBFIFS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.331"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(178..435)
                     /locus_tag="N7A07_004680"
     CDS             complement(178..435)
                     /locus_tag="N7A07_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479178.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(432..929)
                     /locus_tag="N7A07_004681"
     CDS             complement(432..929)
                     /locus_tag="N7A07_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU2479179.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1132..1569)
                     /locus_tag="N7A07_004682"
     CDS             complement(1132..1569)
                     /locus_tag="N7A07_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2479180.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1566..2063)
                     /gene="rrrD"
                     /locus_tag="N7A07_004683"
     CDS             complement(1566..2063)
                     /gene="rrrD"
                     /locus_tag="N7A07_004683"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU2479181.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2063..2278)
                     /locus_tag="N7A07_004684"
     CDS             complement(2063..2278)
                     /locus_tag="N7A07_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2479182.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(2355..2627)
                     /locus_tag="N7A07_004685"
     CDS             complement(2355..2627)
                     /locus_tag="N7A07_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2479183.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            complement(2668..2847)
                     /locus_tag="N7A07_004686"
     CDS             complement(2668..2847)
                     /locus_tag="N7A07_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2479184.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            complement(2984..>3224)
                     /locus_tag="N7A07_004687"
     CDS             complement(2984..>3224)
                     /locus_tag="N7A07_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU2479185.1"
                     /translation="FGAFDNEWHALAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSA
                     FAADKLHVTDITRNATYPVLIDSIAVEVNSTDTAA"
BASE COUNT          744 a          863 c          758 g          859 t
ORIGIN      
        1 cgcagaagga attccccgtc gggctacggt catggttaat gcggaaatac agcgacgata
       61 cagcgcatga tgtgtcaggc ttgaatacct ttatccgtta aaagggatat cagttaagtt
      121 atcccgtgta gggtataagc cattatcaag cccaccagta gatgggcttt gtaatggcta
      181 cttcgctttt gcttccgctc gcttacgccg gcgctcttct ttcctctcgg cttttgccat
      241 gtccatgaat gcctgcatga tcgagttccg catcatgtag ctaacaaagt gatgattgac
      301 acagccgttg aggcgcagct gctcgccaaa ctcatccacc gaggccaatg cttccatcat
      361 acccttctcg cctttcatga actctgagaa gtcgcgcccc gctctggagg cgcattcaat
      421 gacacgatca ctcatcccgg aagcccgggg atcgaaatct gcaactggtt agccagggag
      481 ttaatttcag cgatcaaacc aggtttcgtg tagcgccatg ccgcgaggcc ttgtccacag
      541 aagctcgcca tgtctttttt ctgatcaaac tcatgacatt tcatgttgag ctgcgcactt
      601 aagctgttcc gatgatgaag ctctccggtg aagtaatcat cgaggacttt ataggctgca
      661 tatttaaatc cggggtttag ccatgctgcg taatcataag caacgaactt tccgccataa
      721 gttccaccgt gcgcaccgcg ttccgtaaaa accacagatt cgtggttttt ctccagctca
      781 gccaagaact ctttggtctg cttgtttcgc aggtagtggt acggagattc agattcactt
      841 ttaccactgg ctttccacat atcagtgagg cagatcatgc catcttcacc gatacggatt
      901 ggttgattga agagggttaa tgatttcata gcgtgtacct actctttgaa atgaaccttt
      961 gccgcacagg aaaccagccc accgaggctc gccagcacta actggtatcc tcaaaggccc
     1021 attccaaagg ggcaggttcg gtgtaaaaaa catgcgttgc ggtacgcatt tattgcaaaa
     1081 agccccgcat cgcgaggctc attaaatgga ctttgtgatt tgcaaaaaaa attatttcag
     1141 gcattgcgtc ctgatgtact cctgcaggta gttaacctgc gcggttatct tgtcgattcc
     1201 acctcggaga cggtaataat tgagttcagc atctgctgta agtcctgggc tttctccatc
     1261 gcccatgctg ctggctccgg tcgttgactt tgcacaggtg gcggagactt gcaggcgctt
     1321 acgcccagca gaaacatcag cacggagact ttcgatagtc gcattagcat cagcaagctc
     1381 ctttgtgtat ctggcgtcaa gttctgctac atcacgttgc cgcttctgca tatcagcgat
     1441 gatggatgtg gctttatcgc gctgctcttt gtaggcgatg gcgttatcac ggtaatgatt
     1501 aacagcccat gacaggcagg cgataatgca gataaccaga gcggagataa taacggttac
     1561 cctgctcatt gttgccccca caaacagact tcacgctcaa tctcgcggcg agtcatcagc
     1621 cctttccatt gcttaccgcc agcgtatgtc cagcgccgta gctgatcaca tgcgcctttg
     1681 atatcgccct ggtttatttt gcgaagaagc gtcgatgttc tgaaattgcc agcgcccacg
     1741 ttgtaaacga acgagtaaag agcgccgcgc gttgtttccg gtatatcgac gttgatgtac
     1801 gggttaattt gtctggcgac cgtggcaagg tctttattca ggagggcttt gcattctgct
     1861 tcggtatacg ttttaccggg catgatgtct tttccggtgt gtccgtgaca tacagtccat
     1921 acgccaacga tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccacct
     1981 ggaccagtga tgagcacaga cgctatggca acagccccac caccaatagc agctgcaaca
     2041 gccttgcgta atgacggcga cattattcac ctctcgcagc cttacgctta tcttctttaa
     2101 tcttgaaata aagatttgtc agatacgtca gcaggccaaa cagcagactt cccagcacac
     2161 ctattgccac ccactgggac ggagagactt tgtccagcag ctgcagtaac cagtatcccg
     2221 tccccaccgc tgacgtggtg tatgacacac ctgttgtgat tttttccatc tggtacatac
     2281 cccgtctccc gttatccgga agctcacaac atgaaaaagg ccagcagctg tttactgatg
     2341 gccctgactc cccgttacag catcatgacc gattcgggtt gaggttcagt cgcatcggcg
     2401 accggtgatt caggctgaac ttcaccgctc tctgcggtgg tatctcccgc ttcagtcggt
     2461 ggctctgcct gtacaccaag cagctcatcc agaatggcat caacctctgc atcaagacgc
     2521 gcttccaggt tatggcgaag tttctgtttc agtgcgctcc ggacttcttc agagcgcagg
     2581 acttccttca ctgcttcagc agtgaccagg gatgtaattt ctgacatggg attttctcgt
     2641 cgaaaggtgt gattaagaaa gttgccgcta aatgagcggc tcttcgggtt tgcttccggc
     2701 tgactgactg gcgctgattt tctcagcggc ccttttgtca atctgtctgc gccagaagtc
     2761 acgcatggcc cggtatccac ccgaaaggag atacagcaca cagaccaccg tacagaagta
     2821 cagcattaac tggttcagaa atgtcataat ttctttccgt tattgttgac aataagaact
     2881 gttttcattt aaaaaaccag agcacgaaag tatcgttcct ttattttttc tccataggta
     2941 ttaccaccgc cagcgtccat tcctgtcgct ggcggttttt ttatcatgcc gcagtgtctg
     3001 tgctgttcac ttccaccgca atgctgtcaa tcagcaccgg gtaagtcgca ttcctggtaa
     3061 tgtctgtcac atgcagttta tccgccgcaa aggcactgac cggtgactgc gtcagcgtga
     3121 acggtgtacc atcctgacca tcaataaccg gcgtcacctg aaggctgtta ttcccggcaa
     3181 agcggaaagc cagcgcatgc cattcgttat caaatgcgcc aaag
//
LOCUS       DBFIFS010000331         3141 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.332, whole genome shotgun sequence.
ACCESSION   DBFIFS010000331 DBFIFS010000000
VERSION     DBFIFS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.332"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(351..427)
                     /locus_tag="N7A07_004688"
     tRNA            complement(351..427)
                     /locus_tag="N7A07_004688"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Asp,seq:gtc)
     gene            complement(560..1291)
                     /gene="dnaQ"
                     /locus_tag="N7A07_004689"
     CDS             complement(560..1291)
                     /gene="dnaQ"
                     /locus_tag="N7A07_004689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU2479186.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1356..1823
                     /gene="rnhA"
                     /locus_tag="N7A07_004690"
     CDS             1356..1823
                     /gene="rnhA"
                     /locus_tag="N7A07_004690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU2479187.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1820..2542)
                     /locus_tag="N7A07_004691"
     CDS             complement(1820..2542)
                     /locus_tag="N7A07_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2479188.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2576..>3141
                     /gene="gloB"
                     /locus_tag="N7A07_004692"
     CDS             2576..>3141
                     /gene="gloB"
                     /locus_tag="N7A07_004692"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU2479189.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDY"
BASE COUNT          864 a          743 c          721 g          813 t
ORIGIN      
        1 aacaaagctt ttttgaattc ataattggac actccctcgc ctgtcataat gattagaata
       61 tttagcattg ataacggact ctaattatat accccactta aataataaca aacaaaatca
      121 atttgaaata tacattcatt taatcaatat aaatttattc tctggaaata tatttaagct
      181 gaatggttcg atatcattat tgaattttaa tgaatgagac aacaagcgag aatcacgcac
      241 ttacgccaga gtaaaatcat tgaacagaga aatggtggaa ttcaaataca aaaaaaaccg
      301 caaaattaaa aatcttgcgg ttctctgaac tcattttcat gaggtgaatt tggcggaacg
      361 gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta
      421 ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt acggattgcc tcacccttcg
      481 tcaacggttt ttctcatctt ttgaatcatt tgctgcaaaa atcgcccaag tcgctatttt
      541 tagcgccttt tacatgtatt tatgcccgcc agaggcagct tccgcccttt ttctgcacca
      601 gatcgagacg ggcttcatgt gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact
      661 tacttgcctg acgtacaagg cgctggattg ttgcttcacc ttgttgctgt tgtgtctctc
      721 cttccatcgc aaaagccatc gacgtttgac caccggtcat cgccagataa acttccgcaa
      781 ggatctgggc atcgagtaat gccccgtgca gcgttcgttt actgttatct atttcgtagc
      841 gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa cattttcctc gccaccgcaa
      901 ggctatcggt gaccttacag aaggtattag tcttcggaat atcgcgctta agcaacgaga
      961 actcgtagtc cataaagccg atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc
     1021 ggatatagtc catgaactca tcggctactt cggcaaacgt gggcttatcg agcaaaaatt
     1081 catcggcaat accatgtacg ccaaaggctt ccggatccac cagccgatcg ggtttgagat
     1141 aaacatggaa gttattgccc gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa
     1201 tgatcttgtg gccttcatag tgcgcaccaa tctggttcat accggtggtt tcggtatcga
     1261 gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat ttatgtcaga cttgtcgttt
     1321 tacagttcga ttcaattaca ggaagtctac cagagatgct taaacaggta gaaattttca
     1381 ccgatggttc gtgtctgggt aatcctggac ctgggggtta cggcgctatt ttacgctatc
     1441 gcggacgcga gaaaaccttt agcgcaggct acacccgcac caccaacaac cgtatggagt
     1501 tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca ttgcgaagtc attttgagta
     1561 ccgacagcca gtatgtccgc cagggtatca cccagtggat ccataactgg aaaaaacgtg
     1621 gctggaaaac cgcagacaaa aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg
     1681 ctgcattggg gcagcatcaa atcaaatggg aatgggttaa aggccatgcc ggacacccgg
     1741 aaaacgaacg ctgtgatgaa ctggctcgtg ccgcggcgat gaatcccaca ctggaagata
     1801 cgggctacca agtcgaagtt taagcctgtg gtttacgaca ttgccgggtg gctccaaccg
     1861 cctggcgaat tcgtggcttg tttttactct gtttcatcgg atttagcgtt aaaggaatag
     1921 tccgtttccg ggcaacaata agttgtaagc aaccaagcgc aggaatatgc gcattcaaaa
     1981 gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg gctggcgtgt agcacttcaa
     2041 aattcaacaa agagagccag tccagttgcc gcatcagagt aaacatccgg ctgttatagg
     2101 gcgaggtttt gcgcaatacc ggcacaagtt tgcgtaatcc cattaaacta atgggattga
     2161 agccactaat gaccagccag ccatcatcaa tcaatacccg atcggcttca cgcaataaac
     2221 gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag acaaacatca acggatttat
     2281 cggcaaaagg aagatgaagt gggtccgcct gcacctggac aggcattcct tgcgcagaaa
     2341 cattcacttg atgagaaacc gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa
     2401 tcttaagcag ataaaaacca tacattttag tgaaccacgg gttgagctgg cgctccagcg
     2461 ccttgcgata aagctctccc cagggcaaat cgccccagcg atcaggagcc acgacagttt
     2521 gagggactct tgccggtttc atcacaacct tccgtttcac actgagaggt aatctatgaa
     2581 tcttaacagt attcccgcct ttgatgacaa ttacatctgg gttttgaatg atgaagcagg
     2641 tcgctgcctg attgtcgatc ccggagacgc agagccagta ttaaacgcca ttgccgccaa
     2701 taactggcaa ccggaggcca tatttctcac ccaccatcat cacgatcacg ttggcggcgt
     2761 aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat ggtccacaag agacacaaga
     2821 taagggaaca acacaggtag tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt
     2881 tagtgtaatt gccacgccgg gtcacacttt aggacatatc tgttacttca gtaaacctta
     2941 tctattttgc ggcgacacgc tgttttctgg tgggtgtggt cggttgtttg aagggacagc
     3001 atcacaaatg tatcaatcac ttaaaaagtt aagtgcgtta cctgacgata cattggtatg
     3061 ttgtgctcat gaatatacgt tatcaaatat gaagtttgct ttgagtattc ttccgcacga
     3121 tttgtccata aatgattatt a
//
LOCUS       DBFIFS010000332         3139 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.333, whole genome shotgun sequence.
ACCESSION   DBFIFS010000332 DBFIFS010000000
VERSION     DBFIFS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.333"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..103
                     /locus_tag="N7A07_004693"
     CDS             <1..103
                     /locus_tag="N7A07_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2479190.1"
                     /translation="KHNRTVRGVCREWSVTDNARYSDFSCTIEQVVN"
     gene            103..801
                     /locus_tag="N7A07_004694"
     CDS             103..801
                     /locus_tag="N7A07_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2479191.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(818..1072)
                     /locus_tag="N7A07_004695"
     CDS             complement(818..1072)
                     /locus_tag="N7A07_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2479192.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            1182..1292
                     /locus_tag="N7A07_004696"
     CDS             1182..1292
                     /locus_tag="N7A07_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2479193.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1595..2473
                     /locus_tag="N7A07_004697"
     CDS             1595..2473
                     /locus_tag="N7A07_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HGU2479194.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2527..>3139
                     /locus_tag="N7A07_004698"
     CDS             2527..>3139
                     /locus_tag="N7A07_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2479195.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLS"
BASE COUNT          813 a          673 c          887 g          766 t
ORIGIN      
        1 gaaacataac cggacggtga ggggtgtgtg ccgggagtgg agcgtcacgg ataacgcccg
       61 gtacagtgat ttcagctgta cgattgagca ggtggtgaac tgatgcagga cattcatgaa
      121 gaaagtctga acgagtcggt taaatcagag cagtcaccgc gggtggtgct ctgggaaatt
      181 gacctgacgg tgcagggcgg tgagcggtat tttttctgta atgagctgaa tgaaaaaggg
      241 gaggcggtca cctggcaggg gcggcaatat caggtatacc cgattgacgg cagtggcttt
      301 gagatgaacg ggaagggcag cagtgcccgc ccgtcgctga cggtgtcgaa tctgttcggt
      361 ctggtcaccg gaatggcgga ggacctgcag agcctggtgg gggccacggt ggtccgccgc
      421 cgggtgtatg cccgttttct ggatgcggtg aattttgtgg cgggcaatcc ggaagcggac
      481 ccggagcagg agctgagaga ccgctgggtg gtggagcaga tgtcagagct gacggccatg
      541 acagcctcgt ttgtgctggc aacaccgacg gagacggacg gagcgctgtt tcccggtcgc
      601 atcatgctgg cgaacacctg tatgtgggat taccggggag atgaatgcgg ttatcacggt
      661 cctgcggtgg cggatgagtt cgacaacccc accacggata tccgtaagga cagatgcagc
      721 aagtgcatgc gcgggtgtga gatgcgcggc atggtggcta attttggcgg tttcctttcc
      781 attaataaac tttcgcagta atggattatg cccaccatca ggtgggtttt ttatcttcgc
      841 cgtacatgct gctgagagtc ttaagaagag cctccctaaa tttttcagat tcttgttgag
      901 caaaaccttc tattgatctt ggtgattttt cttcatcaat agcagcttgc aagatcataa
      961 ctatttctga atttaatgag cgtccgtttt tcgatgctct tatggtcagg tcgcgcttga
     1021 ggctatctgg cattcttacg ctatatgggg ctatgtctct aaccttcgtc atacttacac
     1081 cgtgataatc acattgatat cacagtgtat tcaaaaaaac tttgacttga tatagtcaca
     1141 gtgatacgtt tgtgattcat aataactcac gcggtggcga tatggaaaaa gaagttagca
     1201 gaattttggt gagaatacct cagtcgctaa aagatgcgat tacaggaaag gccaaagaag
     1261 aatgccggtc gtttaactcg gaagtgatgt gattgctgga cagcctgaag agagagggga
     1321 taacggtatg agtaaagaat gttgtttttt gcggcattag cgaatcagac gctgatcaaa
     1381 catacattta ctctaaagaa acaggtcgga tgctgtgtag tgactgcgtg tttgacatca
     1441 taagatacaa gtatcttgga tgttctgcca gcattagcaa tataggtgaa gtgtacgaag
     1501 ggaaagatat aactgataga gcagaaagtt gaagccccaa ctgctgtaac agtcagggct
     1561 tcgttatcaa caaatcggct taggaaatat tgacatgaaa agtatagcaa aggcacaaaa
     1621 cgatttcact atcttcaaat tcggcgacag tgaaatccgc gtcatcaaca agtgcggtga
     1681 gccgtggttt gtagctaaag atgtttgtga tgctttagct ttgactaact cacgcaaggc
     1741 gcttactgca cttgatgacg atgaaaaggg agtaacttta agttacaccc ttggtggtga
     1801 gcagaatcta agcattgtga gcgaatcagg tatgtataca ttggttctgc gctgccgcga
     1861 tgcagtcaat aaaggttcag tcccgcacaa attccgcaag tgggtaacag cagaagttct
     1921 gccttcaatt cgcaaacatg gcgagtatgt aaaaggaaag aaaaccactg ttgaggaaag
     1981 aacaccgcta cgcgatgcag taaacatgct ggtaggaaag aaaggacttc gcaatgacga
     2041 tgcatacaat atggttcatc agcgttttgg tattgacagc attgatgagc tttcaattga
     2101 acaaatcccg cttgccgtag agtacatcca cagggtagtg cttgaaggtg aattcattgg
     2161 caaacaagag aagagcacca acgagctttc cgcaaaagaa gcaaacagcc ttgtatggct
     2221 atgggattat gccaaccgct cacaggcatt attccgcgaa ctgtatccgg cgctaaaaca
     2281 aattcaatcg aactattccg gcagatgcta cgactacggt catgagttct cgtatgttat
     2341 tggaatggcg agagacgttt taattaatca cacacgagat gttgatatca atgagccaga
     2401 cggaccaacg aatctttccg catggatgag acttaagaat aaagaattac ctccttcacc
     2461 gcatcactac taacagattg ccaacgaaat gacccagctt cggctgggtt tttatcagga
     2521 gttctcatgc tctatagcaa tatattggcg cacgcccggc gatgtgcacc agcggagtcg
     2581 tgcggcttcg tggtgagaac gccggaaggg gaaagatatt ttccctgcgt gaatatctcc
     2641 ggtgagccgg aagattattt ccggatgtcg ccggaggact ggctgcgggc agaaatgcag
     2701 ggggagattg tggcactggt ccacagccac cccggtggtc tgccctggct gagcgaggcc
     2761 gaccggcggc tgcagataaa aagtgcactg tcctggtggc tggtctgccg gggggaaatt
     2821 cataaattcc gctgtgtgcc acatctgaca ggacggcgct ttgagcacgg ggtgacggac
     2881 tgttacacgc tgttccggga tgcctaccat ctggcgggaa ttgatatgcc ggattttgag
     2941 cgtgaggatg actggtggcg caacggtcag aacctttacc tggacaatat ggcggtcacc
     3001 ggcttttacc gggtgcccct gtcctctgca caggcgggcg atatcctgct gtgctgcttt
     3061 ggcgcatcgg tggccaatca tgccgccata tactgcggca acggtgaact gcttcaccat
     3121 ctgcctgaac aactgagta
//
LOCUS       DBFIFS010000333         3074 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.334, whole genome shotgun sequence.
ACCESSION   DBFIFS010000333 DBFIFS010000000
VERSION     DBFIFS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.334"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1573)
                     /locus_tag="N7A07_004699"
     CDS             complement(<1..1573)
                     /locus_tag="N7A07_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU2479196.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKIS"
     gene            complement(1623..1925)
                     /locus_tag="N7A07_004700"
                     /pseudo
     CDS             complement(1623..1925)
                     /locus_tag="N7A07_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(1952..2374)
                     /locus_tag="N7A07_004701"
     CDS             complement(1952..2374)
                     /locus_tag="N7A07_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2479197.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(2388..>3074)
                     /locus_tag="N7A07_004702"
     CDS             complement(2388..>3074)
                     /locus_tag="N7A07_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2479198.1"
                     /translation="KGDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDAD
                     WTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWV
                     SSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKVTPTSGTVAKGKTTTLT
                     VSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAE
                     VTVTEAGAAG"
BASE COUNT          612 a          981 c          713 g          768 t
ORIGIN      
        1 ggctgatttt ctcattcgca ccgctgtgtt tttccagcgt cctgagctca gcttcaaggg
       61 caagcagagc ctctcttgcc tgctcctctt ccctctcccc ggcagagcgc gttttcggtg
      121 atgtatgctt tttacctgtc agctcttcag ccagacggct gacggcttcc tgctgccccg
      181 gacctttgct gacgcctgtt gcacgcgagc ggttgatgta ccccatttcc ccctggcgta
      241 tacgcgcatc ccgttccgca atggattttc tcagcgccag ttcatcgcgt tttgttttct
      301 caataaatac gcggttctct tctgccagtt caccaaacaa cgcaccaacg ccgggcacat
      361 tctttgtcgt ttcccaggct gactgaataa attcagccag cgccagatcc ccctgcacaa
      421 gcagcagctt cacctgttca acggttcctg ccaccacgtc agtgatcaga ctgagtgccc
      481 ccagtgtatg atcacctatc catgcccatg cgtcagaagt ccaggtttta acatcgtccc
      541 agattttttc caccggcgtg gccgctttat caagttgctc cagacgtgca ttcatgacat
      601 ccgcaaacag ggacatcgcc tccgtcaccg cagcctgttt acctttcgtg cgctcaagct
      661 catcaatatg gcgtaactgg gaaacgctca ggaagttata ctgctgattc agggaggcca
      721 gcgccttcac cggatctgct gcaatccctt caaaggcttt ttccaccttc ccggcatcgt
      781 cccccaccgt ctgcagccat ctctgagagg tttcccccat gatccgtagc tgcccggcgg
      841 tatatttccc gctttctgcc agacgggcca gattttctgc cgcctgtctg ataccaccac
      901 cggcttcatc gctgatcacc ccggccattt tccacaattc tgccgttgtg gtggcagcag
      961 cccctccggt cagggtcagt gaacgcaata aggcccggtc agcctgctct gcctgccagg
     1021 cggcggcagc aagcgcggcc agtacggcaa ccccgccacc tgccgccaca cgggccaccg
     1081 acataaatcg tcccagctca ccggcattcc gggcattttc agccagtgca tttgccgtat
     1141 ctgacagcga ctcctctgat gattcagagg catccctgat cccgagaagt tcctccttca
     1201 gcagggtaag caggctgagc ggtccaccga atgaatcgct gatctgcccc ccctgctgca
     1261 gcatgataag gaagggattc tgaccaccgg caagctgagt gacaatatcc gtgaactgtg
     1321 cgggcagtgt gcgcatggca gccttatact gtccgactga tatcccggct ttttgtgcag
     1381 ccagcgcctg tcggctcaga ctctgctcag cagcatcagc ctgttttctg aaagactgac
     1441 tgactttccc ggacatcaaa tcagcaagcg cactggtttc acccagctct ttttttaccc
     1501 gcgctgcctc ttcagaaaaa cgggcagaat ccagtgtcag cacggctgtc agatcggcaa
     1561 aattacctgc catcgtggtc acctcctgaa atgtcctctg ataccatcaa taactgtcac
     1621 aacctccttc cctcctcccc gaaacggact ccaccggcga ggcccgccgc cttctgcatc
     1681 agtacatcat tttcgtccgg cgtctccgcc tgtccttcct ctgcctccgg agcgaacagg
     1741 ctgaaatccg ccggatgcat atccggatcg caaaaaaaca ggctgagtac ggcgtacatc
     1801 agcccggaaa aatgcatatc cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt
     1861 cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca gtcggctctc
     1921 tccatctcac gcgccagttt caggacaaag ttcagctcgc cttcgaatgc ttttttgatg
     1981 ttaccggctc agtcgcttct gttttcccgg ttggttcagg atcggcatcg tgccggttat
     2041 ccagcatacc tgaaagataa agcacccggt tcgttgcctg attcagtgca tcagcaggcc
     2101 atcccagcat cacttcacgg cggatctgct gcatctctgt ctccggagag gccagagtgc
     2161 ctttgaggga atgggaatgc catagcgaca tcgccacaag cagggatgcc gtttccagat
     2221 atcgctggtt aatgtgcacg acatcatgct tcgttgtctc ctgttgttct gcgtctgaaa
     2281 caaactttat ataatcaaac cgctgcagcg cagacaattc ggaaagcgtg acggacacac
     2341 cgttatattc aaattgttct gttttcagaa acatgtatta cctccgttta ccctgcagcg
     2401 cccgcttcag taacggtgac ttcagccact gcggcgaact gaccatttcc gctcaccaca
     2461 gggatctgca ccttacctgt cgccacgccg tttaccgtaa ttgtcatatc tttcacacta
     2521 atggtggctt tcgacggatc ggcggaaacc gctctgaacg tcttgtcggt tgcactttcc
     2581 ggctcaaaag aaaccgtcag ggtggttgtt ttcccttttg ccaccgtacc ggatgtcggt
     2641 gtcaccttaa ttgcagtggc cggcgtaatt tcgctgcgtt cttccgccac ggaaggtttg
     2701 cccacgttag tgactttcac cgtgcgggtg atcacttctt tcgccgtcac ggccttaccg
     2761 atactgctga cccagccacg gaacacatcc accgtgccat ttgggaaacg gattttatag
     2821 gcccgcacat ccccgctttc aaaccagcct ataagccctt tctgaccttc ttctcccggt
     2881 ttccaggcca gcgtaaaact ggtatctcct gcagacttct gcccctgccc ggtcgcggtc
     2941 cagtccgcgt cttcatcatc caggtagtta tcatcgtagg gttctgccgt catctcgccc
     3001 ggcgtcagat ccttcacctt agccagtcgc tgccagtcat cgtctgacaa cgggtttgca
     3061 taagcatcac cctt
//
LOCUS       DBFIFS010000334         2990 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.335, whole genome shotgun sequence.
ACCESSION   DBFIFS010000334 DBFIFS010000000
VERSION     DBFIFS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.335"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            321..668
                     /locus_tag="N7A07_004703"
     CDS             321..668
                     /locus_tag="N7A07_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479199.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
     gene            678..992
                     /locus_tag="N7A07_004704"
     CDS             678..992
                     /locus_tag="N7A07_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGU2479200.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(1102..1374)
                     /gene="rcnR"
                     /locus_tag="N7A07_004705"
     CDS             complement(1102..1374)
                     /gene="rcnR"
                     /locus_tag="N7A07_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU2479201.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            1495..2352
                     /locus_tag="N7A07_004706"
     CDS             1495..2352
                     /locus_tag="N7A07_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU2479202.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
                     QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGA
                     TLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMG
                     VHGFMGIMR"
     gene            2570..2908
                     /gene="rcnB"
                     /locus_tag="N7A07_004707"
     CDS             2570..2908
                     /gene="rcnB"
                     /locus_tag="N7A07_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU2479203.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
BASE COUNT          855 a          683 c          641 g          811 t
ORIGIN      
        1 gacacattaa acgattacaa taatcaaacc tatattaagg tgaatagaag gtattcgaat
       61 gagtcatttc taatattttt agtcttacgg gcaaaggcta tctattacct aacatgcaat
      121 ggaaagcgga aaagtatgta gatagtgcaa cttgttccct gaactgctgt ttatccaacc
      181 tattgctatg aaaatacgta ataatttcaa acctaaacaa taccattgct aaacaaatga
      241 aaacttgttc gttttactaa ttaatagttt tataatgact accccaaaat aggtaatcca
      301 aatccaagca aagggaaaat atgaagagta aaatttttgc tgcacttctg ctactacctc
      361 tatctactta tgcaagctcc tataaatgtg atgctcttca gcagggtcaa attggcagca
      421 atgggaaaac cgttacggca caatcctcca tcgatattga cggcaaacag atgcatttta
      481 gcttgaacgg agtgagttgg gatctctcat atattggtga aaaacagatt gcaaagatgt
      541 atagcaccga aagcgggaac gtctctgtat ctttcattga tgccaatccc atcatcttcg
      601 tgctacatct cgtaaacgat gatggtagaa aaggcactta caacatcagt gaatgttctg
      661 aattataaga ggctaaaatg aaaaaactac tcgttaccgt catctttgtg gcaatgatgc
      721 caaatgcatt cgcactaagt cacggagaca gcgcaacact taaagaaggt acatttaact
      781 gtaaaaagtt aactgacttt tacgagatga tcagttacat ccaggacaat gaccaacagg
      841 ctatgctgag tctcattaca tccaataaat gtcgcgtgtt agatgaatca atgacggtag
      901 aaatccagag cgtagatgac aaaggttttg tttcattcat aactccaggt ggtcatggtg
      961 gctgggctgt gaagcaattt ttcgaaaatt aatcaaacta taacctcgaa tggcgtgtgt
     1021 cctcacgcca tttactctaa ttcagcttaa cgtcacttgt ccctctattt attgcctcaa
     1081 ctacggccat attaggcact ttcatttgat ataagaatcc agcaccttca gaacaacatc
     1141 cagatcttct tcacgtttta gctcatcccc ctggtgaacg atatgttccg ttagatgacc
     1201 tttaatcact tcccgcatca gaccgtttac cgcgccacgg atagcagcaa tctgttgtaa
     1261 aactgcagcg cattcgtgcg gctcgtcgag cattttcttg agcgccacga cctggccctg
     1321 aatcttactg gcacgcgctt tcagtttctg tttatcacgg attgtatgag acatggcaac
     1381 acctggttaa caagaacatg aaaaatcata gcactattaa tctactgggg ggtagtatca
     1441 ggtactgggg gggagtagaa tcagattgcc gaattaatac taagaattat tatcatgacc
     1501 gaatttacaa ctcttcttca gcaaggaaac gcctggttct tcatccccag cgccatctta
     1561 cttggtgcgc ttcatggcct ggaaccaggg cactcaaaaa cgatgatggc ggcgtttatc
     1621 atcgccatca aaggcaccat taaacaggcg gtgatgctcg gactggcagc aaccatttcg
     1681 cataccgcag tggtctggtt gattgccttt ggcgggatgg tgatcagcaa gcgctttact
     1741 gctcaatcag cagaaccgtg gctccagttg atttccgcag tgatcattat tagcaccgct
     1801 ttctggatgt tctggcgtac ctggcgcggc gaacgcaact ggctggagaa tatgcatgag
     1861 catcatcacc acgatcacga acatcatcaa gaccacgacc acgaccacga ccacgaccac
     1921 gaccacgacc acgaacatca tcaccatcat gaacatggcg acaacgaaga gtatcaggat
     1981 gcccatgcac gagcccatgc caatgacatt aaacgacgct ttgatggtag agaggtcacc
     2041 aactggcaaa ttttgttatt tggcttaaca ggtggcctta ttccctgccc ggcagcaatt
     2101 accgtgctgt tgatttgcat tcagttgaaa gccctgacac tgggcgcaac actggtcgtc
     2161 agtttcagca tcggtctggc actaacgctg gttacagtag gcgttggagc ggcaatcagt
     2221 gttcaacaag ttgcaaaacg ctggagcgga tttaacactc tcgctaaacg cgccccctat
     2281 ttttccagtc tgttaattgg tttagtcggg gtgtatatgg gagtgcatgg ctttatgggt
     2341 attatgagat aaaacaatca cctgggcttc gctatccttt tagcccagga tgattcttaa
     2401 atcagcttat tccgctcact tctcgctcaa tgaggatgct tttgctacat cctttcattt
     2461 acacttttta cgaaatcatg ggatcactaa caaaatatcg cttgtcagtt atattgtatg
     2521 gcaggaaaga tatgcgactg atattacaga tccccaaagt ggagagttta tgaccattaa
     2581 aaataagatg ttgctgggtg cgcttttgct ggttaccagt gccgcctggg ccgcaccagc
     2641 caccgcgggt tcgaccaata cctcgggaat ttctaagtat gagttaagca gtttcattgc
     2701 tgactttaag cattacaaac caggggacac cgtaccagag atgtaccgta ccgatgagta
     2761 caacattaag cagtggcagt tgcgtaacct gcccgcgccg gatgccggga cgcactggac
     2821 ctatatgggt ggcgcgtacg tgttgatcag cgacaccgac ggtaaaatca ttaaagccta
     2881 cgacggtgag attttttatc atcgctaaaa aaagccccct cggagagagg gggaaatgca
     2941 gacaccttat tatttcttat tattagccac ttgctcatct tgcttgttat
//
LOCUS       DBFIFS010000335         2888 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.336, whole genome shotgun sequence.
ACCESSION   DBFIFS010000335 DBFIFS010000000
VERSION     DBFIFS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.336"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..2076
                     /gene="iha"
                     /locus_tag="N7A07_004708"
     CDS             <1..2076
                     /gene="iha"
                     /locus_tag="N7A07_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU2479204.1"
                     /translation="LASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAASVSVI
                     SQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGVRQGG
                     SSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNADKWL
                     SSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSLSDTAA
                     TRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGYDRTLR
                     YERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRELKESN
                     LILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTD
                     ALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISG
                     VSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDN
                     TNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHM
                     VNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDA
                     GLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERN
                     YWMSLNYQF"
     gene            complement(2294..2444)
                     /locus_tag="N7A07_004709"
                     /pseudo
     CDS             complement(2294..2444)
                     /locus_tag="N7A07_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2656..2888)
                     /locus_tag="N7A07_004710"
                     /pseudo
     CDS             complement(2656..2888)
                     /locus_tag="N7A07_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
BASE COUNT          744 a          677 c          788 g          679 t
ORIGIN      
        1 ctggcttccg tagtcattcc ctgtctcgga ttttcagcca gcagcatagc tgctgcagag
       61 gatgtgatga ttgtctcggc atccggctat gagaaaaagc tgactaacgc agccgccagt
      121 gtttctgtga ttagccagga ggaattgcag tccagccagt accacgatct ggcggaggct
      181 ctgagatcag tagagggtgt ggatgttgaa agtggtacgg gtaaaaccgg agggctggaa
      241 atcagcatcc gaggaatgcc agccagttac acgctgatac tgattgatgg tgttcgtcag
      301 ggcggaagca gtgacgtgac tcccaacggt ttttctgcca tgaataccgg gttcatgccc
      361 cctctggccg ccattgagcg tattgaggtt atcagggggc cgatgtccac actgtatggc
      421 tctgatgcga tgggcggtgt ggtgaatatc attaccagaa agaatgcaga caaatggctc
      481 tcttccgtca atgcagggct gaatctgcag gaaagcaaca aatggggtaa cagcagccag
      541 tttaatttct ggagcagtgg tccccttgtg gatgattctg tcagcctgca ggtacgcggt
      601 agcacacaac agcgtcaggg ttcatcggtc acatcactga gcgatacagc agccacgcgt
      661 attccttatc ccacggagtc acagaattat aatcttggtg cacgtcttga ctggaaggcg
      721 tcggagcagg atgtgctctg gtttgatatg gataccaccc ggcagcgtta tgataaccgg
      781 gatgggcaac tggggagtct gacgggggga tatgaccgga ccctgcgcta tgagcgaaac
      841 aaaatttcag ctggctatga tcatactttc accttcggaa catggaaatc gtatctgaac
      901 tggaacgaga cagaaaataa aggtcgtgag cttgtacgca gtgtactgaa gcgcgacaaa
      961 tgggggcttg ccggtcagcc gcgggagctt aaggaatcga accttatcct gaattcatta
     1021 ctgcttaccc ctctgggaga atctcatctg gttacggtgg ggggcgagtt tcagagctcg
     1081 tccatgaaag acggagttgt ccttgccagc acaggtgaaa ctttccggca gaaaagctgg
     1141 tcggtatttg ctgaggatga gtggcatctc acggatgcac ttgcgctgac tgcgggcagc
     1201 cgctatgaac atcatgagca attcggggga cacttcagtc cgcgtgcata tctggtctgg
     1261 gatgtggcag atgcctggac gctgaaaggc ggtgtgacca cgggatataa ggcacccaga
     1321 atggggcagc tacataaagg gattagtggt gtgtccgggc agggaaaaac aaatctactt
     1381 ggtaaccccg acctgaagcc ggaagagagc gtcagttatg aggctggggt gtattacgat
     1441 aaccccgccg gtctgaatgc caatgtcaca ggttttatga ctgacttctc caacaagatt
     1501 gtctcttatt ccataaatga taacaccaat agctatgtaa acagcggaaa ggcccggttg
     1561 cacggtgtgg aatttgccgg cacattgccg ctgtggtcag aggatgtcac gctgtcactg
     1621 aattacacct ggacccgaag tgaacaacgt gatggtgata acaaaggtgc gccgctgagt
     1681 tatacccctg aacacatggt gaatgcgaaa ctgaactggc agatcaccga agaggtggca
     1741 tcatggctgg gtgcccgtta tcgcgggaaa acaccacgtt tcacccagaa ttattcgtca
     1801 ctgagcgctg tacagaagaa agtgtatgat gagaaaggag aatacctgaa agcctggacg
     1861 gtggtggatg caggtctgtc gtggaagatg acggatgccc tgacgctgaa tgctgcggtg
     1921 aataacctgc tcaacaagga ttacagtgac gtgagcctgt acagtgccgg taagagtacg
     1981 ctgtatgccg gtgattactt ccagacggga tcatcaacaa caggatatgt gatacctgag
     2041 cgaaattact ggatgtcgct gaactatcag ttctgataat aacaaaacgc tatcactgac
     2101 ggtagaatac gttgccactg caactcctgg cggaacagtg gcaacgtatt aggttaagtg
     2161 catttccgat ccgctaatga gatttcgtta ccaacaacta atatcgtcac aggaaatgca
     2221 cggattattt ttaacttatc atttacatac ttgtccagag tgtaagcgca ccgtgaagga
     2281 cgtggggtaa aaattagttt acagagagag tgacgttcca gggcaacaac tctttcatgc
     2341 ggttggcagg ccaggtgttg attacactga tcacgtggcg tagccacgtt tccggctcga
     2401 ttccgttaag ttttgcagct accgatcagg ctgtacatca ctgccgcact atcgctcgtc
     2461 atctcaaagt ccggtctcgt cagcaggaag gtatcattct ctcccgccat ttttccaggg
     2521 gcccggtcag ataagtccct ttgtctatcg ctgactcctg actcataacc cggttagcag
     2581 aatgcagggt caccacctcg ccacgaccaa atccaaataa gtcaattgca ccttctcaat
     2641 cgccattttg tcagtaagcg tacagcctga actgatggta tcttcaccat caatgacaac
     2701 ggtgatcgca attttactga cgttcgccgg aacacgatcc agtgctatct caatgctggc
     2761 ctgctgcgaa ccggtaacga gcctgactgc cccctcagga gaagacaaat tattataaaa
     2821 gataaagtca gaatcgccac tgacctttcc ctgagcatta agcatgaaca ggcaggtatc
     2881 gggttcgc
//
LOCUS       DBFIFS010000336         2887 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.337, whole genome shotgun sequence.
ACCESSION   DBFIFS010000336 DBFIFS010000000
VERSION     DBFIFS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.337"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..480
                     /locus_tag="N7A07_004711"
     CDS             <1..480
                     /locus_tag="N7A07_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2479205.1"
                     /translation="SARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVN
                     FVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMW
                     TYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRSMVANFGGFLSINKLSQ"
     gene            491..1234
                     /locus_tag="N7A07_004712"
     CDS             491..1234
                     /locus_tag="N7A07_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2479206.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            1231..1812
                     /locus_tag="N7A07_004713"
     CDS             1231..1812
                     /locus_tag="N7A07_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2479207.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            2154..>2887
                     /locus_tag="N7A07_004714"
     CDS             2154..>2887
                     /locus_tag="N7A07_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2479208.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQV"
BASE COUNT          634 a          711 c          923 g          619 t
ORIGIN      
        1 agtgcccgcc cgtcgctgac ggtgtccaat ctgttcggtc ttgtcaccgg gatggcggag
       61 gatttgcaga gcctggtggg tgccacggtg gtccgccgcc gggtgtatgc ccgttttctg
      121 gatgcggtga attttgtggc gggcaatccg gaagcggacc cggagcagga gctgagagac
      181 cgctgggtgg tggagcagat gtcagagctg acggccatga cagcctcgtt tgtgctggca
      241 acaccgacgg agacggacgg agcgctgttt cccggtcgca ttatgctggc gaatacctgt
      301 atgtggacct accgctctga tgagtgtggt tacacgggcg gggcagtggc ggatgagttc
      361 gacaacccca ccacggatat ccgtaaggac agatgcagca agtgcatgcg cgggtgtgag
      421 atgcgcagca tggtggctaa ttttggcggt ttcctttcca ttaataaact ttcgcagtaa
      481 atcccggttt atgacacaga ctgaatcagc gattctggcg catgcccggc ggtgtgcgcc
      541 tgcggagtcg tgcggcttcg tgataagcac gccggagggg gagtggtata tcccttgtgt
      601 gaatatttct gcagagccgg aggcgtattt tcgtatcgca ccggaagact ggctgcgggc
      661 agagatgcag ggggagattg tggcactggt ccacagtcat cccggtgggc tgccctggct
      721 gagcgaggct gaccggcggc tgcagataaa aagcgcactg ccctggtggc tggtctgccg
      781 gggtgacatt cacaaattcc gctgtgtgcc acatctgaca ggacggcgct ttgagcacgg
      841 ggtgacggac tgttacacgc tgttccggga tgcttatcat ctggcgggga ctgatatgcc
      901 ggattttgag cgtgaggatg actggtggcg caatggtcag aacctgtacc tggacaatat
      961 ggaggcgact ggtttttaca ggatttccct gccttccgca cagcctggcg atatcctgct
     1021 gtgctgcttt ggcgcatcgg tggccaatca tgccgccata tactgcggca acggtgaact
     1081 gcttcaccat ctgcctgaac aactgagtaa acgggagagg tattccgaaa aatggcaacg
     1141 acgaacgcat tcagcctggc gtcaccgcca ctggcacgta tctgccttca cggggattta
     1201 caacgatttg gccgccgcct cagcctgtat gtgaacacgg cagcggaagc catccgggcg
     1261 ctgtcgttac aggtgccggg ctttcgccgt cagatgaacg aaggctggta ccagatacgt
     1321 attgccggtt atgacacggc accggaggcg gtgtacgccc gtcttcacga acagctgggt
     1381 gagggaacgg tcatccatat tgtgccgcga ctggccgggg ccggaaaggg tggactgcag
     1441 attgtgctgg gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg
     1501 ttatggggtt cagccctggc agccggtggt ttttctgcca ccacgatgct gttttcactt
     1561 ggagccagca tgattctggg cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg
     1621 gattaccgcg caacggataa cggcagacag aacacgtact tttcctcgct ggataacatg
     1681 attgcccagg ggaacccgat gccggtgcct tacggggaaa tgctggttgg ctcccgccgt
     1741 atatcccagg acatcagcac ccgtgatgaa ggtgggggcg gaacggtcgt ggttgtcggg
     1801 cgacagggat aaaacataaa aaaatcccgc agtgatcgcg gagctgcggg gacagacaaa
     1861 tgaagatcaa tgttaaggag ttgtttttgt tactcgggca aaaaaacact aacgcagcga
     1921 aattataagc gccacagtca gtgtgtgaaa atgtgaagat attcagaatt tttatgccat
     1981 taccggtttt aaccaacagg attatcggtg ggcatgaaag aaaaccccgg tatctgctga
     2041 taccggggtt ttctttagca tggcagaaat gtgtttcatg cttttcgggc gaaggatatc
     2101 cgacttctgt acggaatggc aagtggcggt taatttattc aggggaaggc tgtatgggaa
     2161 aaggtggcgg taaggcacac acgcctcgtg aggcgaagga taatctcaaa tccacgcaga
     2221 tgatgagtgt gattgatgcg attggtgagg gaccgataga aggtccggtg aagggactgc
     2281 agagtattct ggtgaacaaa accccactga cggacacgga cggcaatccc gtgatacacg
     2341 gtgtgaccgc ggtctggcgt gccggggagc aggagcagac accaccggaa ggctttgagt
     2401 cctccggagc tgaaaccgga ctgggcgtgg aagtgacgaa ggcaaaaccg gtgacgcgca
     2461 ccattacgtc cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag tcactgttgg
     2521 agaccacctc aaagggcgac cgtaatcact cttctgtccg actgctgatt cagttgcagc
     2581 gtaacggtaa ctgggtgacg gaaaaggatg tcaccattaa cggcaagacc acctcgcagt
     2641 ttctggcgtc ggtgattctg gataatctgc ctgagcggcc ctttaacatc cggatggtcc
     2701 gggagacagc ggacagcacc tcggaccagc tgcagaataa gacgctctgg tcgtcataca
     2761 ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc gattgtgggg ctgcaggtgg
     2821 atgcggagca gtttggcggt cagcagatga cggtgaacta ccatatccgc ggtcgcatca
     2881 tccaggt
//
LOCUS       DBFIFS010000337         2872 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.338, whole genome shotgun sequence.
ACCESSION   DBFIFS010000337 DBFIFS010000000
VERSION     DBFIFS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.338"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..323
                     /locus_tag="N7A07_004715"
     CDS             <1..323
                     /locus_tag="N7A07_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HGU2479209.1"
                     /translation="EGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNNVV
                     IKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHGNYI
                     SDKI"
     gene            339..2819
                     /locus_tag="N7A07_004716"
     CDS             339..2819
                     /locus_tag="N7A07_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU2479210.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
BASE COUNT          838 a          532 c          716 g          786 t
ORIGIN      
        1 atgaaggaaa gaatgcgtta aaatttgcca tgcaaaacag aatcaagttg ttttaccgac
       61 ctgctggtat tgctccggta aataaaacga cttttaaaaa attacgagtg agtcgtagcg
      121 gtaataacgt agtcataaaa aatgactcgc caaattgggt aacgatttct gatgttaagg
      181 ctaataacgt caaagtgaat tatgaaacaa tcatgattgc accacaagaa agtcagaatg
      241 ttagtgaaaa aaataataat gcgaatggct ggcaactgac cattatcgat gatcacggca
      301 actatattag tgacaaaatt taattgcagg agctgcctat gttgagaatg accccgcttg
      361 catcagcaat agtcgcgtta ttgctcggca ttgaagcaca tgcagcagaa gaaacctttg
      421 acacccattt tatgatggga ggaatgaaag gtgagcaagt aacaaatttg cgtcttgatg
      481 ataatcagcc tttacctggg cagtacgaca ttgatattta tgtcaataaa caatggcgtg
      541 gaaaatatga gattatcgtc aaagataacc cacatgaaac ttgcctgacg cgtgaaattg
      601 taaagcggtt aggtataaat agcgataatt ttgcacgcga aaatcagtgt ttaacatttg
      661 agcaacttgt tcagggcgga agctactcct gggatattgg gatttttcgt ttggatctcg
      721 ctgttccgca agcctgggtt gaagagctgg aaaatggtta cgttccgcca gaaaactggg
      781 agcggggaat caatgcgttt tatacgtctt attacgtaag tcagtattac agtgactata
      841 aagcctcggg caatagtaag agcacttatg tgcgttttaa cagtgggcta aatttgctgg
      901 gatggcagct gcattcagat gccagtttta gcaaaacgga taataatcct ggtgagtgga
      961 aaagcaacac gctgtatctg gagcatggat tttctcaaat tctcggtacg ctgcgtattg
     1021 gtgatatgta cacctcagcc gatatatttg attccgttcg cttcaccggg gttcggttat
     1081 ttcgtgatat gcagatgttg ccaaactcga agcaaaattt taccccacgg gtacagggga
     1141 ttgcccagag taacgcgttg gtaactattg aacagaacgg ttttgtcgtt tatcagaaag
     1201 aggttccacc aggtccgttt tcgattagtg atttgcagtt agcgggcgga ggggcggatc
     1261 ttgatgttag tgttaaggaa gccgatggtt ctgtgactac gtatctggtg ccttatgcgg
     1321 cagtacctaa tatgctgcaa cctggtgtat caaaatatga ttttgcggca ggccgtagtc
     1381 atattgaagg tgcgagcaag caaagtgatt ttgtccaggc gggttatcag tatggtttta
     1441 ataatttatt gacgctgtat ggcggcacga tggttgctaa taattactat gcctttaccc
     1501 tcggaacagg ttggaacacg cgtattggcg caatttcagt cgatgcaact aaatcgcaca
     1561 gtaagcaaga caatggtgat gtgtttgacg gacaaagtta tcaaattgcc tataacaaat
     1621 ttttgagcca aacatcaaca cgttttggtc tggcggcctg gcgttattcg tcgcgtgatt
     1681 accgcacatt taacgattat gtgtgggcaa ataataaaga taattatcgt cgtgataaaa
     1741 acgatgtcta tgatattgcc gattattacc agaatgattt tggtcgcaaa aatagctttt
     1801 ccgccaatat gagtcagtca ttgccagaag gctggggatc tgtgtcatta agtactttat
     1861 ggcgtgatta ctgggggcga agcggcagta gtaaagatta tcaactgagt tattccaata
     1921 actggcggcg gataagctat accctcgcgg caagccaggc gtatgacgag aaccacgccg
     1981 aagagaaacg ctttaatatt tttatatcga ttccctttga ctggggcgat gacgttacga
     2041 cgccgcgacg gcaaatatat atgtctaact caacgacctt tgatgatcag ggttttgctt
     2101 caaacaatac cggattatcg ggaaccgttg ggaaccgcga tcagtttaac tatggtatca
     2161 acttgagcca tcaacatcag ggaaatgaaa cgacagctgg ggccaatttg acctggaccg
     2221 caccggccgc aacagtgaat ggcagctata gtcagtcgag tacttatcga caggtcggag
     2281 ccagtgtttc agggggactg gttgcctggt ctggtggcgt taatctggcg aaccgtcttt
     2341 ccgaaacgtt tgctgtgatg catgcgccgg gaatcaaaga tgcttatgtc aatgggcaaa
     2401 aatatcgtac aacaaactgt aatggtgtgg tggtgtacga cggactgaca ccttatcggg
     2461 aaaatcacct gatgatggat gtgtcgcaaa gcgatagcga aacagaatta cgcgggaacc
     2521 gtaaaatgac cgccccttat cgcggcgcgg tcgttctggt tgattttgat accgatcagc
     2581 gtaagccctg gtttataaaa gcattaagat ccgatggaca gccattaacg tttggttatg
     2641 aagtcaatga tatgcatggt cataacattg gtgttgtcgg ccagggcagt cagatattta
     2701 ttcgcaccaa tgaaataccg ccagcggtta atgtggcaat tgataagcaa caagggcttt
     2761 catgcacaat caccttcggt aaagagattg atgaaagtaa aaattatatc tgtcggtgag
     2821 aggtgaaata tgatgttcag aaatagaata ttactaatat ttatattgtg gg
//
LOCUS       DBFIFS010000338         2848 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.339, whole genome shotgun sequence.
ACCESSION   DBFIFS010000338 DBFIFS010000000
VERSION     DBFIFS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.339"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1390
                     /locus_tag="N7A07_004717"
     CDS             <1..1390
                     /locus_tag="N7A07_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU2479211.1"
                     /translation="GLGDRADRGISPLAQEGLVKWALCGHWGQSPRISDLAEQNKIIA
                     YNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKEDLIKLVEFDNK
                     EYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNNGGIVMMQVQKM
                     VKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLDDSTKLSLPLNQ
                     RKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVETGPIGGITSQGI
                     AFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKFNGKIMGTGGFI
                     DISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEITFSGKIALERG
                     LDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKLMDERLFID
                     AAMGFVLPEAAH"
     gene            1404..2555
                     /locus_tag="N7A07_004718"
     CDS             1404..2555
                     /locus_tag="N7A07_004718"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU2479212.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2598..>2848)
                     /locus_tag="N7A07_004719"
     CDS             complement(2598..>2848)
                     /locus_tag="N7A07_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2479213.1"
                     /translation="RISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAK
                     LFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          753 a          651 c          743 g          701 t
ORIGIN      
        1 agggcttggc gatcgcgccg accgcggtat tagtccgctg gcgcaagaag gtctggtgaa
       61 atgggcatta tgcggtcact ggggacaatc gccgcgtatt tctgatctcg cagaacaaaa
      121 taaaattatt gcttataact atccacaagg tgtacttaca caaaccttac gcgccgccgc
      181 agcccaccag cctggtatta ttagtgatat tggcatcggg acatttgtcg atccacgcca
      241 gcaaggcggc aaactgaatg aagtcactaa agaagacctg attaaactgg tcgagtttga
      301 taacaaagaa tatctctatt acaaagcgat tgcgccagat attgctttca ttcgcgctac
      361 cacctgcgac agtgaaggct acgccacttt tgaagatgag gtgatgtatc tcgacgcatt
      421 ggttattgcc caggcggtgc acaataacgg cggtattgtg atgatgcagg tgcagaaaat
      481 ggttaagaaa gccacactgc atcctaaatc tgtccgtatt ccgggttatc tggtggatat
      541 tgtggtggtc gatccggatc aaagccaact atatggcggt gcgccggtta accgctttat
      601 ttctggtgac ttcacccttg atgacagcac caaacttagc ctgcccctaa accaacgtaa
      661 attagttgca cggcgtgcat tattcgaaat gcgtaaaggc gcggtgggga atgtcggcgt
      721 cggtattgct gacggcattg gcctggtcgc ccgggaagaa ggttgtgctg atgactttat
      781 tctgacggta gaaacaggtc cgattggtgg aattacttca cagggtatcg cctttggcgc
      841 gaacgtgaat acccgtgcca ttctggatat gacgtcccag tttgattttt atcacggtgg
      901 tggtctggat gtttgttatt tgagttttgc tgaagtcgac cagcacggta acgtcggcgt
      961 gcataaattc aatggcaaaa tcatgggtac cggtggattt attgatatca gtgccacctc
     1021 gaagaaaatt attttctgcg gcacgttaac tgcgggcagt ttaaaaacag aaattgccga
     1081 tggcaaattg aatatcgtcc aggaaggacg ggtgaagaaa tttattcggg aactaccgga
     1141 aattactttt agcggaaaaa tcgctctcga gcgagggctg gatgttcgtt atatcactga
     1201 gcgcgccgta ttcacgctga aagaagacgg actgcattta atcgaaatcg cccctggcgt
     1261 cgatttacaa aaagatattc tcgacaaaat ggatttcacc ccagtgattt cgccagaact
     1321 caaactgatg gacgaaagat tatttatcga tgcggcgatg ggttttgtcc tgcctgaagc
     1381 ggctcattaa caggagtatc acgatggatt tttctttaac tgaagaacaa gaactgctgc
     1441 tggccagtat tcgcgaactg attacgacta actttccgga agagtatttc cgcacctgcg
     1501 atcaaaacgg gacatatccg cgtgagttta tgcgggcact ggcggataac ggtatttcca
     1561 tgcttggcgt gccggaagaa tttggtggta tccctgctga ttacgtcacc caaatgctgg
     1621 cgctgatgga agtgtcaaaa tgcggtgctc cggcattttt aattaccaac ggtcaatgta
     1681 ttcacagtat gcgccgtttc ggttctgcag agcagctacg taaaacggca gaaagcaccc
     1741 tggaaacggg tgatcccgcc tatgccctgg cgttgacgga accaggtgct ggctcagata
     1801 acaacagtgc cactaccact tacacgcgta aaaacggcaa ggtttacatc aacggacaga
     1861 aaacctttat taccggtgcg aaagagtacc cgtatatgct ggtgctggcg cgcgatccgc
     1921 aaccgaaaga tcccaaaaaa gccttcaccc tgtggtgggt cgactccagt aagcccggca
     1981 ttaagattaa cccgctgcat aaaatcggct ggcatatgct cagcacctgc gaagtctatc
     2041 tcgacaacgt ggaagttgaa gagagcgaca tggtgggcga agaaggaatg ggtttcctca
     2101 atgtgatgta caactttgag atggagcgcc tgatcaacgc cgcgcgcagc accggctttg
     2161 ccgagtgtgc ctttgaagat gccgctcgct atgccaacca gcgtatcgct tttggtaaac
     2221 ccattggtca taaccagatg atccaggaaa aactggcgct gatggcgatt aagattgaca
     2281 acatgcgcaa catggtgctg aaagtggcat ggcaagccga tcagcatcaa tcactgcgca
     2341 ccagcgcggc gctggcaaaa ctgtactgtg cacgtaccgc aatggaagtc attgatgatg
     2401 cgattcaaat catgggcggt ctgggctata ccgatgaggc gcgcgtctcc cgcttctggc
     2461 gtgatgtccg ttgtgaacgt attggcggcg gtacagacga aattatgatt tacgtagcag
     2521 gtcggcagat cctgaaagac tatcagaaca aataatctgc aggcggcgca gcttcttaac
     2581 aaactgcgcc gccagattta tccggcaaga cttaccggtt gaggaaattc agataggatt
     2641 tcttgttccg caaaacagtt tttaaattgc ctgcggtaat cgctgggcga acagccgaca
     2701 tggcgcaaaa acagtttggc aaagtgatcg acattttcat aacccacacg ccaggagatc
     2761 tctgcctgtg ataattcagt attagtgagt gaccattttg cttccgtcat acgccgttga
     2821 ataacatagt taatagggga aatacgat
//
LOCUS       DBFIFS010000339         2830 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.340, whole genome shotgun sequence.
ACCESSION   DBFIFS010000339 DBFIFS010000000
VERSION     DBFIFS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.340"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            289..519
                     /locus_tag="N7A07_004720"
     CDS             289..519
                     /locus_tag="N7A07_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479214.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            571..1932
                     /locus_tag="N7A07_004721"
     CDS             571..1932
                     /locus_tag="N7A07_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGU2479215.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            1979..2542
                     /locus_tag="N7A07_004722"
     CDS             1979..2542
                     /locus_tag="N7A07_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2479216.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2628..>2830)
                     /locus_tag="N7A07_004723"
     CDS             complement(2628..>2830)
                     /locus_tag="N7A07_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479217.1"
                     /translation="HKQENNKTDSNQSSRKKKNSSSSSSSSSSSSSSSSMNNNIISNR
                     QMTGLKRQKPTAECPRNVRGGA"
BASE COUNT          654 a          769 c          822 g          585 t
ORIGIN      
        1 agcagcaggt tcccgcgaaa cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc
       61 tttaaaaatc aggatttatt tcaggctccg gcaatacgca tttatgtgtt ctgtcagtca
      121 tccggctgac aggtaatgag aacgcaggaa cagaaggagg aaaacaccgg aaagcgtcag
      181 atgcagatat gaaaaaagca ggtcagggac ctgcttaatc cacgccgtaa gaattgtgcg
      241 accaattttg acggcattgt taaccaattt gtcataagga atccatttat gactgtaagc
      301 attgtttcac cttccgcagc ggctgtaaag ccccgccgtc atccccgttt cagacgcgaa
      361 gacatccccg ctccggaaat tgaccccgtg ctgaaagcct tcgggcgaca catagcccgg
      421 agttttcacc gggggcgtgg tgtacacatc cccgccatga aaaacacggc tttcgggcag
      481 gtgctccgca cactcgaact gaaacgcgct tttaactgat gaggtgagcc gccccgcgag
      541 gggcggcaac agacggaagg agaacatttt atgacatcag cagcaatcac catgaccgcc
      601 ccggaagccg ccagccctgt gcagatgtac cgcgcgacct actcaccgga tgacaacaaa
      661 ctgcgcctgt atgccgtgtc acgtcttgac ccggagacgt ataaaaaagt gcatgatgcc
      721 ggttttcgct gggcaccaaa acaggcgctg tttgtcgcgc cggcctggac accgggccgg
      781 gaagacgtgc tcctctcact tgccggagag attgaggatg aagacagcac gctcgctgaa
      841 cgtcaggaag cacgggcgga gcggtttacc ggatacagcg gaaagcgggc cagtgaatcc
      901 gcacaggcac ttgatgaagt ggaaagactg gccgcgatga tcccgcccgg tcagcccatt
      961 cttgtggggc atcacagcga acgccgcgcc cgtcgtgatg cgcagcgtat tgaaaacggc
     1021 atgaaacgtg ccgtgatgct ctttgaacgt gcggaatact gggaagaacg ggggcggtca
     1081 gcactgcttc acgcgaagta taaagaacgt ccggacgttc gctggcgtcg tatcaaaaaa
     1141 atcgaagctg atttgcgcaa ggctgaaaag accatcgcgc agtcgcagaa atatctgacg
     1201 atgtggcggg ctgaatcgct ggatctgaat atggcaaaac tcatcagcag tcatgaccat
     1261 atcagcgcct gtttcccgct ggatacgtat ccgcgcccgg cagaaaaaag ccagtatgaa
     1321 gggagccggt cgttatggtc ggccctggat gatgacatca tcaccacgga gcaggcccgc
     1381 gaaattgcga tccgctgtca tgaacggcag attcagcatc agcaacgctg ggttaaccac
     1441 tatcagaacc gcctgaacta tgagcgtgcc atgctggacg aaagcggcgg cgtggttacc
     1501 cggacacagg attttgagcc gggcggacag gttttcagcc ggggcgagtg gctgaccatc
     1561 atccgcgtga acaaaagcaa cggggcggtg agttcagtca caacgccgaa ttacagtttt
     1621 ctcgggtaca gcggcacgat gaaagtgacg cccgatcgca tcacggacta caaagcacca
     1681 tcggcagaag aggctgccgt cgccagccag gccgcgaagc gtccgccggt agtcaactat
     1741 ccgggggaag gtttccggga aatgacaaag gcacagtggg ccgccctgcc ccgggactgt
     1801 aaggccgtgc gcagtgtggc agaagcagaa gaccacgggg cataccgcta ccgccgcaca
     1861 atggacaata atttccgtct ggtgaatgtg tatatcaccg acatgaaaat tacggaaatc
     1921 ccacagaaat aaggtatatc cccccgggaa atccccgggg gatgacggag ataatgacat
     1981 gcacagtcag ttaaaagaac gtatccggct gatgcgcgca aggctggata acgccgcgcc
     2041 ggttgctgaa atccgggctg aatctcagct ttttgtgact cctgccccgg tctgtgatcg
     2101 cctggtgacg ctggcggaga tcagcaaccg tgatcacatt ctggaaccct ctgccggcac
     2161 cggggccata ctgcgggcga ttcgggatac cgcaccggag gccatgtgtg atgcggtgga
     2221 aatcaacagc gggctggtcc ggtatctgcg ggaaaatttt aacggtgtca gggttcagtg
     2281 tggcgacttc atggaatggc agccggtgca gtattacagc cgggttatca ttaatccgcc
     2341 gttcagccac gggcaggata tccggcatat cctgcgggcc ttttccctgt tgcgtccggg
     2401 tggcgtgctg gtcgccgtct gtctgaacgg gccgcgccag caggagaagc tgttaccgtt
     2461 ttctgacgtc cgcgaggagc tgccacgcgg cacgtttgct tataccgatg ttccgacgat
     2521 gattattcgt ctgcgtgcct gatgactccg ggcaggcggg aggataaatg cccgtctgcc
     2581 cgcgccggga aaagaccgcg cgccggtggc gctgaaaggg gccgcgctca cgcgccgccc
     2641 cttacattac gcggacattc tgctgtgggc ttctgtcttt tcagacctgt catttgtctg
     2701 ttactgatga tgttgttgtt catgctactg ctactgctac tgctactgct actgctactg
     2761 ctactgctac tgttcttctt ttttctgctg ctctgattac tgtctgtttt gttgttctcc
     2821 tgcttatgcc
//
LOCUS       DBFIFS010000340         2812 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.341, whole genome shotgun sequence.
ACCESSION   DBFIFS010000340 DBFIFS010000000
VERSION     DBFIFS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.341"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            40..258
                     /locus_tag="N7A07_004724"
     CDS             40..258
                     /locus_tag="N7A07_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU2479218.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            260..817
                     /locus_tag="N7A07_004725"
     CDS             260..817
                     /locus_tag="N7A07_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2479219.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1108..1263
                     /locus_tag="N7A07_004726"
     CDS             1108..1263
                     /locus_tag="N7A07_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2479220.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1383..1727
                     /locus_tag="N7A07_004727"
     CDS             1383..1727
                     /locus_tag="N7A07_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU2479221.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1849..2121
                     /locus_tag="N7A07_004728"
     CDS             1849..2121
                     /locus_tag="N7A07_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479222.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2123..>2812
                     /locus_tag="N7A07_004729"
     CDS             2123..>2812
                     /locus_tag="N7A07_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2479223.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPPHTHQ"
BASE COUNT          742 a          653 c          756 g          661 t
ORIGIN      
        1 ctcagaacat caggaggccg cccgaaaggg cggtaagaaa tgaaacatta tttagaaaaa
       61 aattacccac gaaagagcag aacaacagag tttctgtttt tcattctgtt tatagtgttg
      121 atgataccga tatccccgct attactggtc tggataattg gaaggacatt tgaaccagtt
      181 attgagctat ataccgatgt gacatgggaa tcattcagcg cactgcacaa taaaattaat
      241 ccgtataagg aaaactgata tgagcactat taccagagaa cgcgcggaga ttaaatcata
      301 catcacaggc ttcctgagcg actcggcgca cgataacaag tcttcagaca gcctgctggc
      361 taatgtgttt cgtatcgcgc tggcatcact ggaagcagag ccgatagcaa tggtagtgcc
      421 tgatgaaatg gatttgctta cctgccatct cgacggtgta actaaaacat atgctgatgg
      481 ctggaacgcc tgccgcgtcg ccatgcttca ggccggaaac tttcgggaaa ataagaattc
      541 gtcaaccaac aattttcggg aaatctcgga aacgtcaacc agatctccga taactctgga
      601 tggctggata agctgtactg agcgaatgcc tgaaaagagc cagaacgtgc ttatttcgat
      661 gaatatcgat agcgaggctg ggccattaat atattccgca cgctatctcg gaggcacgtt
      721 ccggcgcgga ggtatagcag ttagtccggg taatgatctt aggcaagcaa cccactggat
      781 gtcgctacca gaaccgccgc aggaggtgaa tcaatgacct ggcctgaagc attcacaacg
      841 gtaggaattg cgatggcggt ggcgctggtg gtgtattcga tttgccgctg gggataaaaa
      901 cggtttgcgg gaaaaggata gttaagtaga attgctgcgg gtgcttgagg ctatctgcct
      961 cgggcatgaa caccaacggc agatagagaa aagccccagt taacattacg cgtcctgcaa
     1021 gacgcttaac attaatctga ggctcaatcc atgctgaaca catgtaggtt agcctcttac
     1081 gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg
     1141 atcgtcatct gtttaaccgt catagtgacg gcactggtaa cgaggaaaga cctctgcgag
     1201 gtgcgaatcc gaaccggtca gacggaggtc gctgtcttcg tagactacga atctgagaag
     1261 taagagacca ggcgggggag taatctcccg ccacctctga tgtgtcaggc atcctcaacg
     1321 cacccgcgct ttaccatact gaaaatgctg tttgaatgtt catctctgaa agaggactat
     1381 gaatgaaaaa ggtattgatt gcagcactta tttccggtgt gtcttttggc gcttttgcac
     1441 agcagggtgg tttccagggg ccagaagcag agcgttcaac agtagcgcag gcaaaagaac
     1501 tgaaggatga tgcatgggtt atccttgaag ggagcatcgt taaaaaagtg ggtgatgaac
     1561 gttatgagtt tcgtgacaat agcgggacaa ttgtcacgga tattgatgac agcgtatggg
     1621 ccgggcagaa tgtttctccg aaagacaaag taagaattga gggtgaaatt gataaagacc
     1681 tgagcagtgt tgaagttgat gtaaaggcac tgaaattatt aaagtaaccg cccctgcttg
     1741 ttaagcccgt cttactgacg ggttttctgt ttgtacattc cggcgtattg ccttacaatt
     1801 cgcgcagtca gcctgaacaa ctgacacctg ctgtcaccgg agaatccgat gacacaacac
     1861 ataaaatccc acaattctga agccgacccg gaaattaagc aggggaggcg ttttcgtgcg
     1921 cctcagtatg gctggtttca ctatctgttc tgtacgatcg atgaggcaga tatgcttcaa
     1981 gaggcgtatc tgcgtcgcgg tgtccgtgtg gagcggagtc tgaacgctga tcgtctgacc
     2041 tggaccgttt ctgtatatct tcctgttcgt gcacatctgc cacggacaca tgcctgctac
     2101 cgtcagcgcg tctggaggta atgtgcgggt attacttcga cctgttctag ttccggaact
     2161 cgggctggtg gtccttaagc ccggtcgtga atcattgcca gtttttcatc gcggcagggt
     2221 gctggtggag ccggaaccga aaaacatgcg ggcgctgcca tctggagcgg ttcctgctgt
     2281 tcgccagccg ctggcggaag ataaatcact gctgccattt ttcagcgatg agcgggtgat
     2341 tcgtgcagct ggcggcgctg gtgcactgtc tgactggtta ttacgtcacg tgaaatcctg
     2401 ccagtggcca cacggcgatt atcatcacag cgaaaccgtt attcacagtt acggtgctgg
     2461 cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgc gaccagacct ccgaatcact
     2521 tgagcaactt actcaacaaa atctgacagc ctggatgatt gacgtcatac gccatgtaat
     2581 gaatggcacg caggagcggg aattatcgct ggctgaatta tcctggtggg cagtctgcaa
     2641 tcaggtggtg gacgcattac ctgaggcagt atcgcgtcgc tctctgggat taccggcgga
     2701 aaaaatccgc tccgtatacc gtgaaagcga catcataccg ggagaacaga ccgccaccag
     2761 catactgaag cagcgcacaa aaaatattgc gctaccgcct cacacccacc ag
//
LOCUS       DBFIFS010000341         2812 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.342, whole genome shotgun sequence.
ACCESSION   DBFIFS010000341 DBFIFS010000000
VERSION     DBFIFS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.342"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..275
                     /locus_tag="N7A07_004730"
     CDS             <1..275
                     /locus_tag="N7A07_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HGU2479224.1"
                     /translation="KNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTF
                     SGMNRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            275..670
                     /locus_tag="N7A07_004731"
     CDS             275..670
                     /locus_tag="N7A07_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU2479225.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSASINPKDDFEEQTRRNLSMLKK"
     gene            987..2194
                     /locus_tag="N7A07_004732"
     CDS             join(987..1308,1308..2194)
                     /locus_tag="N7A07_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
                     /protein_id="HGU2479226.1"
                     /translation="MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYQEGSLTAVAAGEDVVPASELAAAIKQINQLQRLLGKKTMEVEILKEAV
                     EYAQSPKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRQRRDETAD
                     AEVLSRILDIIGDMPAYGYRRVWAILRRQSRNEGLPFVNAKRVYRIMSENSLLLLHDK
                     PSRRQREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCDREAIDWAASTGG
                     YDKATVQDVMLGAIEKRFGDKVPEQSIQWLTDNGSAYRAHETRQFARELNLEPCTTAI
                     SSPQSNGMAERFVKTIKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPR
                     EYRRQRVMLT"
     gene            complement(2247..2690)
                     /locus_tag="N7A07_004733"
                     /pseudo
     CDS             complement(2247..2690)
                     /locus_tag="N7A07_004733"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
BASE COUNT          897 a          581 c          633 g          701 t
ORIGIN      
        1 taaaaaacag gcatgataat gaatatctcc agacattaat aaatgatgtg gctgaaataa
       61 aaaatattac tcatgtaata gacagtgagg tgaaatctct ggcagaaagc atcaggcagg
      121 aaatttcaac attctcagga atgaaccgaa tctacctcac aggcggggga gccgagctta
      181 tttaccctca cataaagcaa tacttcccga atctgaaagt gaacaaggta gatgaacctc
      241 agtttgcatt ggtaaaagca atggtgcatg cgtaatggaa agcagtgatc caaaaaaaag
      301 aaagaaagtt gtggcatatc tgcacccggc actttatccg caggacaacc tgacacaaca
      361 aacaatagac tctctcccag tacaaatgcg gggagatttc tacagacaat cccttatttg
      421 cggagcagct ctttattctg ttgacccccg actgttaaca ctaatcagcg gttttttcag
      481 tgagaaaatc actgcagaaa atctggtgaa acttatagaa caaacaacag gatacacatc
      541 gacatcaatt gatataagtg tgttaaaaaa catcatagag gcatcttcag aaaacaaatc
      601 agcgagcatc aatccgaaag atgatttcga agaacaaacc agacgcaatc tgtctatgtt
      661 aaaaaaatga caaagagggg cagaatatat tctgcccact ctaattttca caatacttat
      721 gttctgtaaa gcatccaact gtagttgaaa aactcaaatg tctttgagtt taatattttt
      781 tacatctatc attcctaaat aagaatgaac actaacaaaa ggggtttata ccaataggag
      841 gtataggaac aggagcatca tgtagctctc gaagaagcag ctcaaaatta ttatgtgagc
      901 tggccccctg aaataccaga cagtagatgt atcttaaaat aagagatagg cttggatata
      961 tgtctaacac tagttctaat tttgagatgg ctggcgtttt gttaggaaaa gaggtgcgta
     1021 aacgtaaaac acctcaggaa aaaatcgcaa ttattcaaca aactatggag ccgggcatga
     1081 cggtgtccca cgtggcgcgt ttgcatggaa ttcagcccag tctgttgttt aagtggaaga
     1141 aacaatacca ggaaggaagc ctcactgctg tggctgccgg tgaagacgtt gttcctgctt
     1201 ctgaactggc cgctgcaata aagcagatta atcagcttca gcgtttactg ggtaagaaaa
     1261 ccatggaagt tgagatcctc aaagaagcgg tggagtatgc acaatcgcga aaatggatag
     1321 cgcatgtgcc cttgttacct tcggacaagg aatagccccg gtaagccgtg ttctgggcgt
     1381 gtcacgtgcg cagttaacat tacggatgaa ggcatctgat aacaagccag ataaacggcg
     1441 tcagaggcgc gatgagactg cggatgcaga agtcttatcg cgcatcctgg atatcattgg
     1501 cgatatgcct gcatacgggt atcgccgtgt ctgggcgata ctcaggcggc agtcccgaaa
     1561 tgaaggacta ccgttcgtga atgcaaagcg tgtttaccgg ataatgagcg aaaatagtct
     1621 gttgttgtta catgacaaac catcacgacg gcagcgggaa cataaaggcc gaatatcggt
     1681 gaaggaaagc gaccagcgtt ggtgttccga tggctttgag ttcggttgcg atgatggcga
     1741 aaaactgcgt gttacgttcg tcctggactg ttgcgatcgt gaagccatag actgggccgc
     1801 aagcacggga ggttacgata aagcgacagt gcaggatgtg atgttaggag cgatagaaaa
     1861 acgctttggc gataaagtac cggaacagtc gatccagtgg ctgacagata acggttcagc
     1921 gtacagagcg catgaaacac ggcagttcgc cagagagctg aatctggagc cctgcacaac
     1981 ggcaatcagt agtcctcaga gtaatggaat ggcagaacgg ttcgtgaaaa cgataaagga
     2041 agactacatc gcgttcatgc cgaaaccgaa tgtaagaacg gcattgcata accttgcagt
     2101 ggcgatcgag cattacaatg aaaaccatcc gcatagtgcg ttgggttatc gctctccgcg
     2161 agaatatcga cgtcagcggg taatgttaac ttaagataca acccctgtct ggaaataagg
     2221 gggcaagaac actcaaaatt attatgatat tcggctccat cactcaaatt gtagtaaaat
     2281 cgtcctgaca atgcatctgg caaagagacc ccattagtta gatttcttat aattggaata
     2341 aattttatag ttcccatttc ttgcattatc tcgctagtca aaagatggct ctcatatccg
     2401 acacctccaa ctcgaccacg agctttataa caatatcgat ccgtacaaat gaccaaaact
     2461 ctatcagctt ccctaattga tctctccata aatactgaca tatcctcccc tcccctaata
     2521 ttccattggt caagaaatgc atcaattcca ttatttctca gacagccagc taattgagcg
     2581 acccattgtt tatgttcggg agaatcccaa gagtatgtaa taaatacttt tggcggtctc
     2641 ctctgcgact ggagcaactg tctttgaatt atctttttag ttcttaacac attaattaag
     2701 ttaacatctt ttaacgccca gtgggttcgg gttagttcaa aagtatcagg ttctactcct
     2761 aaataaggga aaatatcgaa taactgcttt tgggtaatga agggaatatc at
//
LOCUS       DBFIFS010000342         2798 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.343, whole genome shotgun sequence.
ACCESSION   DBFIFS010000342 DBFIFS010000000
VERSION     DBFIFS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.343"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(76..492)
                     /locus_tag="N7A07_004734"
                     /pseudo
     CDS             complement(76..492)
                     /locus_tag="N7A07_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(492..677)
                     /locus_tag="N7A07_004735"
     CDS             complement(492..677)
                     /locus_tag="N7A07_004735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479227.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            854..>2798
                     /gene="katP"
                     /locus_tag="N7A07_004736"
     CDS             854..>2798
                     /gene="katP"
                     /locus_tag="N7A07_004736"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGU2479228.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLD"
BASE COUNT          741 a          642 c          728 g          687 t
ORIGIN      
        1 ttggtgctgc aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt
       61 tcgagttcgt agtcgcacat ctgcctggcg gcagatttcc tgtatcacat ccgcagcaat
      121 gcggctcatc tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg
      181 aagtgtgaac acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat
      241 ccactgtgcg ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca
      301 gacctttttt atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag
      361 cgtggtcccg caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc
      421 ttccggttgt ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa
      481 acggggaagc atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc
      541 gggcagtaca tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg
      601 cagcgggcta tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt
      661 ttgtttctga catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg
      721 tcagttgcgg cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg
      781 tatatcctgt tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc
      841 cggagctatt cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg
      901 gagcttttct accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga
      961 aacactggat ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga
     1021 ttttgattat gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa
     1081 agatttgctg acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt
     1141 ctttattcgt atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg
     1201 cgccagtggt ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct
     1261 ggataaagcc cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg
     1321 gggagacctg atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct
     1381 gggatttgct ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga
     1441 caacaagcct cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc
     1501 cacgcagatg ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct
     1561 ggcttccgcg aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac
     1621 tgtggccctg atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga
     1681 aaaatgtatt ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa
     1741 aaataaatgt ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg
     1801 gtcgacatcg ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg
     1861 ggagctgcac aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat
     1921 agttcaggac gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat
     1981 tgctcttaaa gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa
     2041 agcttttgag caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc
     2101 ggcagcccga tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct
     2161 tcctgcggcg gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt
     2221 tatggatttg ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac
     2281 atttcgtgtg actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga
     2341 aattaactgg gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc
     2401 attacagcgt gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt
     2461 aattgttctt tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact
     2521 tgagattccc tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc
     2581 attcagtgta ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag
     2641 tcatatatcg ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc
     2701 tgaaatgacg gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc
     2761 gttgggagtg tttaccgata cccctggtgt tctggata
//
LOCUS       DBFIFS010000343         2790 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.344, whole genome shotgun sequence.
ACCESSION   DBFIFS010000343 DBFIFS010000000
VERSION     DBFIFS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.344"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            278..1334
                     /locus_tag="N7A07_004737"
                     /pseudo
     CDS             278..1334
                     /locus_tag="N7A07_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1553..2143)
                     /gene="espM1"
                     /locus_tag="N7A07_004738"
     CDS             complement(1553..2143)
                     /gene="espM1"
                     /locus_tag="N7A07_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HGU2479229.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            2327..>2790
                     /gene="nleG"
                     /locus_tag="N7A07_004739"
     CDS             2327..>2790
                     /gene="nleG"
                     /locus_tag="N7A07_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HGU2479230.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNS"
BASE COUNT          836 a          629 c          548 g          777 t
ORIGIN      
        1 gtgttgacaa gtaatttcca gaagacaaat taaatgaaaa tggcataatt ttatccctaa
       61 agcatatatc ttacttttcg ataattaaca aaaagccatc ctccacagca atactccatc
      121 agaataattg taggattatt ttaatctaat ttataagcat aaaatatata ctcactaaat
      181 agctgcgcag aatagtagat catttagagg gaactcaacc cgattgtgcg atctgatcaa
      241 tcgccaaatc aatacaaatc accaaccgga ctgagcaatg tcgatcatac caccaatttc
      301 ccgtgacgaa cgacgcctaa tacagaaagc catccataaa aggctgacag ccatgctgat
      361 gctgcaccgg ggcgaccgtg tcagcgacgt tgcccgcaca ctatgctgcg cccgttcctc
      421 tgttgggcgc tggattaact agttcacaca gtcgggtgtt gagggactga aatcattacc
      481 tgccgagcga gcccgccgct ggccgtttgc gcatatctac acactattac gtgaacaggt
      541 aaaatattca ctcgcgactt tggctacaat tattcacgct ggagtacaga actgctgaca
      601 ataaaaatca atgagataac cggctgcctg ttacatgctg gaaccattcg ccgctggttg
      661 tcgtctgtgg ggcttgtgtg gcgaagggct gcgccaactc tacgtatccg tgccccgcct
      721 aaagataaaa agatggcaac aatccataac gcactggacg aatgcagcac agagcatccg
      781 gtcttttatg aagatgaagt atttatccat cttaatccca aaatcggtgc ggactggaaa
      841 ctgctcggga agcaaaaacg aggggtcacg ccggaacaaa atgaaaaata ttctctggac
      901 gtagcgctgc acagcgggac aggttaagtc agctgtgtgg gcgccaacag caaaagttcg
      961 gcgctgttca tcagactgct gaagcggctt aaagctacat actgtcggac gaaaaccatc
     1021 acgctgctcg aggacaacta cattatccac aaaggccggg aaacccagcg ctggctgaag
     1081 gataaaccga agtgcagagt catttatcag ccggtttaat ctccatgggt gaatcatttt
     1141 gaatagctat ggcagatact tcacgccaca ataacgcgta atcatcagtg ccgctcaatg
     1201 tggcaactat tgaaaaagtt cgccatttta tggaatccgt cagtccattc cccgaaggca
     1261 aacatgggct ggcaaaaatg tgcgatatta ggcgcagcta tttagttatt ccgtcatttc
     1321 tcccgcccgt gtaaacacta ctgaatcttg tcgttgtgac aaagctataa atagtcaatc
     1381 acagcacgtt tctgcgtacg acgcgctgca attttcttta cgatttataa ctgtacaggt
     1441 gaactatgag ttatttggat acatacatat agtgtaacgc ctgttgtata ttttactcat
     1501 atctttttac aggatgaaaa ccaatgatac tggcgcatag cctcctccaa ccttacccct
     1561 gtataacacg actcatataa cctgggattt catttcgtag ttttacaata tcaacacccg
     1621 gggcgatatg tttctgataa aacttgtctg caagcacgtt tgctatgtaa tctgttgtac
     1681 tgttttttac agctcggctc ataccatcgc aaagagcatc catttttttg ccaaaagaag
     1741 catttcctat tattatatga ttaatagaac tttgtgccgc tttaacatca agagttagtt
     1801 caaactcctt acttacaaga gaaaagacca ttttcctctc ttcagaagaa atttttgcat
     1861 ttttttgaag gcagcaatta tcaatctctt ggttgataac ccttgacgga tatactgtcc
     1921 gttcatcact tacccatcca tgaatatctt tagtactctg cctgcttacc gtctccagta
     1981 tacagccgct aaatttgtta acattaaagc ggatagagcg ctctcccatt gaacacttca
     2041 cgacctcatc attcttccca cggatggtgc cccggaaaga attatcttta tgataactga
     2101 taccaaaaga gctgaaggac acacctgtcg catttactgg cataacaaat ccttttcatt
     2161 aaaatttgct tacaaaaatt gtatacaatc attttaatta aaaaaataca tgtgtaaata
     2221 gcattcgttc aaaatttaat tttttgtttg aatcctatca catatttagt atcttgactc
     2281 ctgtcaccaa tatggttcaa taacatagac taaagaggga gaaaatatgc ctgtcatatt
     2341 aaacttttcg agtgaaagag tattgtctga aagtgaactg gaggctttac gtcatgtcgg
     2401 aagagttagt cagagtgaac agcttgttgt gagaggccgc actatgagac ttcatcatat
     2461 ttcatttatg gacagtttta gcgtggaacc ggtttccggc ggactgttgg atcgccttag
     2521 tgcaagaggg catcgccttc tcgcagaaaa tctggagata cagcttaaca gaggccatac
     2581 ttttttgcag gcattccgtt tatatatgga acagagccgt gcaaccccat gtacgcgcca
     2641 aaacgtcagt agtgcaatac aaaacaaaat taactctcat gcatttactg ttagtcatca
     2701 agacttttcc tgtcatgaac aacacctgaa ttgcccgata acattatgca ttcctgaaac
     2761 tggtgttttt gtcagaaatg caaaaaactc
//
LOCUS       DBFIFS010000344         2736 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.345, whole genome shotgun sequence.
ACCESSION   DBFIFS010000344 DBFIFS010000000
VERSION     DBFIFS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.345"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            151..2298
                     /locus_tag="N7A07_004740"
     CDS             151..2298
                     /locus_tag="N7A07_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU2479231.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
     gene            2607..2736
                     /locus_tag="N7A07_004741"
                     /pseudo
     CDS             2607..2736
                     /locus_tag="N7A07_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
BASE COUNT          819 a          586 c          604 g          727 t
ORIGIN      
        1 taaaatatct atcaaaaaac atgattaagg tcaaaaatgt ttgatattta caatttatga
       61 agatgacaat aattatagat atatgagaac ataaatgaaa ataattatca ttacagcaat
      121 catttgtact ttgtattaat gagggatgaa atgttatata atataccttg tcgaatttat
      181 atcctttcca ctctgtcatt atgcatttct gggatagttt ctactgcaac cgcaacttct
      241 tcagaaacaa aaatcagcaa cgaagagacg ctcgtcgtga ccacgaatcg ttcggcaagc
      301 aacctttggg aaagcccggc gactatacag gttattgacc aacaaacatt gcagaactcc
      361 accaatgcct ccatagccga taatttgcag gacatccccg gagtagagat aacagacaac
      421 tccttggcag gccgtaaaca aatccgcatt cgtggcgaag catcctcccg tgttttaatt
      481 ctcattgatg gtcaggaggt aacttatcag cgcgccggag ataattatgg tgtgggactg
      541 ttgatagatg agtctgcgct ggagcgtgtt gaggtagtga aaggtccata ttccgtactg
      601 tacggttcac aggcaattgg cggtattgtt aacttcatca ccaaaaaggg aggtgacaaa
      661 cttgcatctg gagttgtgaa agctgtttat aattccgcaa cagcaggctg ggaagaatca
      721 atcgcggtcc aggggagcat cggtggattt gattatcgca tcaacggtag ttattctgat
      781 cagggcaatc gtgatacgcc ggatggacgt ctgccgaata ccaactatcg taacaatagt
      841 cagggtgtat ggttgggtta taactccgga aaccatcgtt ttggcctctc gcttgatcgc
      901 tacagactcg cgacacaaac ttactatgag gatccagacg gaagttatga ggcatttagt
      961 gtcaaaatac ctaaacttga acgagagaaa gttggggtat tctatgacac agacgtggac
     1021 ggtgactatc taaaaaaaat tcatttcgac gcgtatgagc agaccatcca gcgccaattt
     1081 gccaacgaag taaaaacgac acagcctgtt cccagtccga tgattcaggc tctgaccgtt
     1141 cataacaaga ctgacaccca tgataagcaa tacactcagg cggtcacatt gcagagtcac
     1201 ttttcgctgc ctgctaataa tgaacttgtt accggtgcac agtacaaaca agacagggtc
     1261 agccaaaggt ccggtggcat gacctcaagc aaatctctga ccggcttcat taataaggaa
     1321 acacgaactc gctcctatta tgagtcagag caaagtacag tctcactatt cgcacaaaat
     1381 gactggcaat tcgccgatca ctggacatgg acaatgggag ttcgccaata ctggctttct
     1441 tcaaagttga cgcgtggtga cggagtatca tataccgcag gcattataag cgatacctct
     1501 cttgccagag agtctgcgag tgatcacgaa atggtaacat ctacaagcct gcgctattca
     1561 ggtttcgata acctggagtt acgcgctgcg ttcgcgcaag gctacgtatt tcccacactc
     1621 tcccagcttt ttatgcagac atctgcgggc ggcagtgtca catacggaaa tcctgatctt
     1681 aaggctgaac actccaataa ctttgaatta ggtgcacgat ataatggtaa tcagtggctg
     1741 attgacagcg cagtttacta ctcagaagct aaagattata ttgcaagtct gatctgtgat
     1801 ggcagtatag tttgcaatgg taacaccaac tcctcccgta gtagctacta ttattatgac
     1861 aatattgatc gggcaaaaac atggggactg gaaataagcg cggaatataa tggctgggtt
     1921 ttctcgccat atatcagtgg caatttaatt cgtcggcaat atgaaacttc aacattaaaa
     1981 acaactaata caggtgaacc agcgataaac ggacgtatag ggctgaaaca tactcttgtg
     2041 atgggtcagg ccaacataat ctctgatgtt tttattcgtg ctgcctctag tgcaaaagat
     2101 gacagtaacg gtaccgaaac aaatgttccg ggctgggcca ctctcaactt tgcagtaaat
     2161 acagaattcg gtaacgagga tcagtaccgg attaacctgg cactcaataa cctgacagac
     2221 aaacgctacc gtacagcaca tgaaactatt cctgcagcag gttttaatgc agctataggt
     2281 tttgtatgga atttctgatg acaaaaacaa tcgttatgga attctttgtc ctgatctgcc
     2341 cgctttgcgg gcaggctaac gaacgtattg tgtttgttga aggtttttcg acgttgtttt
     2401 ccatctgtgg gtgaatattt attcttcccg ccagcatgac tctatactca ttgctacgac
     2461 aatgacttat aaagctggtt actgaaccta tacctttgta attcaaaagt gatatcagtt
     2521 cacaacgcaa tactattttt agattattta ctctcaccca gatactgaat caatggataa
     2581 taccttttga tttatcccga cgcggaagaa ggtctcagtc cggattatgg gggaaaattt
     2641 ctcaatggtc cggtatcaac aaccagggcc gggaggatta caggaaacga gattttgccc
     2701 cggacagccc cgaaataaaa ttcagtgatc gcaggg
//
LOCUS       DBFIFS010000345         2731 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.346, whole genome shotgun sequence.
ACCESSION   DBFIFS010000345 DBFIFS010000000
VERSION     DBFIFS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.346"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(12..>2731)
                     /gene="eae"
                     /locus_tag="N7A07_004742"
     CDS             complement(12..>2731)
                     /gene="eae"
                     /locus_tag="N7A07_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HGU2479232.1"
                     /translation="FFYVNQNSFANGENYFKLGSDSKLLTHDSYQNRLFYTLKTGETV
                     ADLSKSQDINLSTIWSLNKHLYSSESEMMKAAPGQQIILPLKKLPFEYSALPLLGSAP
                     LVAAGGVAGHTNKLTKMSPDVTKSNMTDDKALNYAAQQAASLGSQLQSRSLNGDYAKD
                     TALGIAGNQASSQLQAWLQHYGTAEVNLQSGNNFDGSSLDFLLPFYDSEKMLAFGQVG
                     ARYIDSRFTANLGAGQRFFLPANMLGYNVFIDQDFSGDNTRLGIGGEYWRDYFKSSVN
                     GYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLIYEQYYGDNVALFNS
                     DKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKSWSQQIEP
                     QYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNIPHDINGTEHSTQKIQLIVKSK
                     YGLDRIVWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNIYKVTARAYDRNGN
                     SSNNVQLTITVLSNGQVVDQVGVTDFTADKTSAKADNADTITYTATVKKNGVAQANVP
                     VSFNIVSGTATLGANSAKTDANGKATVTLKSSTPGQVVVSAKTAEMTSALNASAVIFF
                     DQTKASITEIKADKTTAVANGKDAIKYTVKVMKNGQPVNNQSVTFSTNFGMFNGKSQT
                     QATTGNDGRATITLTSSSAGKATVSATVSDGAEVKATEVTFFDELKIDNKVDIIGNNV
                     RGELPNIWLQYGQFKLKASGGDGTYSWYSENTSIATVDASGKVTLNGKGSVVIKATSG
                     DKQTVSYTIKAPSYMIKVDKQAYYADAMSICKNLLPSTQTVLSDIYDSWGAANKYSHY
                     SSMNSITAWIKQTSSEQRSGVSSTYNLITQNPLPGVNVNTPNVYAVCVE"
BASE COUNT          768 a          633 c          533 g          797 t
ORIGIN      
        1 tggttatgga attattctac acaaaccgca tagacatttg gagtattaac attaacccca
       61 ggaagagggt tttgtgttat taggttataa gtgcttgata ctccagaacg ctgctcacta
      121 gatgtctgtt taatccaagc agttattgag ttcatagaac tataatggct atatttattt
      181 gcagcccccc atgagtcata aatatctgac aataccgtct gtgtggatgg taataaattt
      241 ttgcaaatgg acatagcatc agcataatag gcttgcttat ccacttttat catatacgac
      301 ggtgctttta tagtgtaact tactgtttgc ttatcaccag atgtggcttt aattacgaca
      361 ctgcctttac cattcaaagt gactttccct gatgcatcga cagtcgcgat actggtattt
      421 tctgaatacc atgaatatgt accatcacca ccgcttgctt tcagtttaaa ctgaccatat
      481 tgcagccaaa tattaggcaa ctcgcctctg acattgttac caataatatc aaccttgttg
      541 tcaattttca gttcatcaaa aaaagtgacc tcagtcgctt taacctcagc cccatcactg
      601 actgtcgcac taacagtcgc tttaccggcg gaactggaag ttagtgttat cgtcgcacga
      661 ccatcatttc ccgtggttgc ttgcgtttga gacttaccgt tgaacatccc aaagtttgtt
      721 gagaatgtaa cggattgatt attaactggc tgaccgtttt tcataacttt tacagtatat
      781 ttaatagcat ccttaccatt tgctactgca gttgtcttat cagccttaat ctcagtaatg
      841 ctggccttgg tttgatcaaa aaatataacc gcactggcat taagtgctga agtcatctcc
      901 gcggttttag cagacacgac gacctgtcct ggcgtactcg acttcaacgt tacggttgcc
      961 ttaccgttag catccgtttt ggcactattt gccccaagag ttgcagttcc tgaaacaata
     1021 ttaaatgaaa cagggacatt agcctgagct accccattct ttttcaccgt cgcggtataa
     1081 gtaatggtat cggcgttatc cgctttagcc gaagtcttat ccgccgtaaa gtccgttacc
     1141 ccaacctggt cgacaacttg accattcgac agaacggtaa tagtaagctg tacattgtta
     1201 gagctattgc cattacggtc ataggcgcga gccgtcactt tataaatatt gctgccacct
     1261 tgcacataag caggcaaaat agcctggtag tcttgtgcgc tttggcttcc gctatgctga
     1321 atctgaccgc cctgactgcg taatgcacta tcatcccaga cgatacgatc cagaccgtat
     1381 ttgctcttaa cgatcaactg aatcttctgc gtactgtgtt cagtaccatt aatatcatgc
     1441 ggaatattca gagaaagaat atcctgcttc ttgtactcca gaataatatt gttattacgc
     1501 tgaaccagat cgtaacggct gcctgataat gttcttaact cgttaacata ctgtggttca
     1561 atttgctgag accacgattt atcaaactga taacggaact gcattgagta aaggagatca
     1621 ttttcattac ccgtaccatg acggtaatcg atccccatcg tcaccagagg aatcggagta
     1681 tagtttacac caacggtcgc cgcaccagga ttcgactgca gcttatcaga attaaacaaa
     1741 gcaacattat caccataata ctgctcatat atcagcttgg cgcctaatgc cggatatgac
     1801 ggtagatagc cattaaaacg gatatcgaag ccatttgctg ggcgctcatc atagtctttc
     1861 ttattgtatg actcatgcca gccgctcatg cggaaatagc cgttaacgct acttttgaaa
     1921 tagtctcgcc agtattcgcc accaatacct aaacgggtat tatcaccaga aaaatcctga
     1981 tcaatgaaga cgttatagcc caacatgttt gcaggaagga aaaaacgctg acccgcacct
     2041 aaatttgccg taaagcggga gtcaatgtaa cgcgctccga cctgaccaaa tgccagcatt
     2101 ttttcggaat catagaacgg taataagaag tccagtgaac taccgtcaaa gttattacca
     2161 ctctgcagat taacctctgc cgttccataa tgttgtaacc aggcctgcaa ctgtgacgaa
     2221 gcctggttac cagcgatacc aagagcggta tctttcgcgt aatcgccgtt cagagatcgc
     2281 gactgaagct ggctaccgag actcgccgcc tgttgtgccg cataatttaa tgccttgtca
     2341 tcggtcatgt tgcttttggt cacgtccggg gacattttag tcagtttatt cgtgtgacca
     2401 gcaacaccac ctgcagcaac aagaggtgcc gaacctaaaa gtggtagtgc actgtattca
     2461 aagggaagtt ttttgagtgg caaaatgatc tgctgaccag gcgcggcctt catcatttcg
     2521 ctttcagaac tgtataaatg cttattcaac gaccaaatcg tcgataaatt aatatcttgc
     2581 gatttagaaa gatcggcaac agtttcacca gttttcaacg tataaaaaag gcgattctga
     2641 tagctatcat gagttaacag ttttgaatcc gaacccaatt taaaataatt ttcaccattt
     2701 gcaaatgaat tctgattaac ataaaaaaac a
//
LOCUS       DBFIFS010000346         2704 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.347, whole genome shotgun sequence.
ACCESSION   DBFIFS010000346 DBFIFS010000000
VERSION     DBFIFS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.347"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            81..287
                     /gene="ybcW"
                     /locus_tag="N7A07_004743"
     CDS             81..287
                     /gene="ybcW"
                     /locus_tag="N7A07_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU2479233.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(452..646)
                     /locus_tag="N7A07_004744"
     CDS             complement(452..646)
                     /locus_tag="N7A07_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2479234.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            1035..1580
                     /locus_tag="N7A07_004745"
     CDS             1035..1580
                     /locus_tag="N7A07_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU2479235.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1555..>2704
                     /locus_tag="N7A07_004746"
     CDS             1555..>2704
                     /locus_tag="N7A07_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU2479236.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKE"
BASE COUNT          679 a          603 c          730 g          692 t
ORIGIN      
        1 acgtgctcaa atcttcatac agaaagattt gaagtaatat tttaaccgct agatgaagag
       61 caagcgcatg gagcgacaaa atgaataaag aacaatctgc tgatgatccc tccgtggatc
      121 tgattcgtgt aaaaaatatg cttaatagca ccatttctat gagttaccct gatgttgtaa
      181 ttgcatgtat agaacataag gtgtctctgg aagcattcag ggcaattgag gcagcgttgg
      241 tgaagcacga taataatatg aaggattatt ccctggtggt tgactgatca ccataactgc
      301 taatcattca aactacttaa cctgtgacag agccaacacg cagtctgtca ctgtcaggaa
      361 agtggtaaaa ctgcaactca attactgcaa tgccctcgta attaagtgaa tttacaatat
      421 cgtcctgttc ggagggaaga acgcgggatg ttcattcttc atcactttta attgatgtat
      481 atgctctctt ttctgacgtt agcctccgac ggcaggcttc aatgacccag gctgagaaat
      541 ttccggaccc tttttgatca agagcgatgt taatttgttc aatcatttgg ttaggaaagc
      601 ggatgttgcg ggttgttgtt ctgcgggttc tgttcttcgt tgacatgagg ttgccccgta
      661 ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcggatcaa
      721 ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcg tagatgcacg
      781 ttgtgacatg tagatgataa ttattatcat tttgcgggtc ctttccggcg atccgacagg
      841 ttacggggcg gcgacctcgc ggtttttcac tatttatgaa aattttccgg tttaaggcgt
      901 ttccgttctt cttcgccgta acctaatgtt tttatttaaa acaccccctg aaaagaaagg
      961 aaacgacagg tgctgaaaac gggctttttg gcctctgtcg tttcctttct ctgtttttgt
     1021 ccgtggaatg aacaatggaa gtcaacaaaa agcagctggc tgacattttc ggcgcgagta
     1081 tccgtaccat tcagaactgg caggagcagg gaatgcccgt tctgcggggt ggtggcaagg
     1141 gtaatgaggt actttatgac tctgccgccg ccataaaatg gtatgccgaa agggatgctg
     1201 aaattgagaa cgaaaagctg cgccgggaag ttgaagaact gaggcaggac agcgagacag
     1261 atctccagcc agggactatt gagtacgaac gccatcgact tacgcgtgcg caggccgacg
     1321 cacaggaact gaagaatgcc agagactcag ctgaagtggt ggaaaccgca ttctgtactt
     1381 tcgtgctgtc gcggatcgca ggtgaaattg ccagtattct cgacgggatc cccctgtcgg
     1441 tgcagcggcg ttttccggaa ctggaaaacc gacatgttga tttcctgaaa cgggatatca
     1501 tcaaagccat gaacaaagca gccgcgctgg atgaactgat accggggttg ctgagtgaat
     1561 atatcgaaca gtcaggttaa caggctgcgg cattttgtcc gcgccgggct tcgctcactg
     1621 ttcaggccgg agccacagac cgccgttgaa tgggcggatg ccagttacta tctcccgaaa
     1681 gaatccgcat accaggaagg gcgctgggaa acactgccct ttcagcgggc catcatgaat
     1741 gcgatgggca gtgactacat ccgcgaggtg aatgtggtga agtctgcccg tgttggttat
     1801 tccaaaatgc tgctgggtgt ttatgcctac ttcatagagc ataagcagcg caacaccctt
     1861 atctggttgc cgacggatgg tgatgccgag aactttatga aaacccacgt tgagccgact
     1921 attcgtgata ttccgtcgct gctggcgctg gccccgtggt atggcaaaaa gcaccgggat
     1981 aacacgctca ccatgaagcg tttcaccaat gggcgtggct tctggtgcct gggcggtaaa
     2041 gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg gttatgatga acttgctgcc
     2101 tttgatgagg atattgaaca ggaaggctct ccgacgttcc tgggcgataa gcgtattgaa
     2161 ggctcggtct ggccaaagtc catccgtggc tccacgccca aagtgagagg cacctgccag
     2221 attgagcgtg cagccagtga atccccgcat tttatgcgtt ttcatgttgc ctgcccgcac
     2281 tgcggggagg agcagtacct taaatttggc gataaagaga cgccgtttgg cctcaaatgg
     2341 acgccggatg atccctccag cgtgttttat ctctgcgagc ataatgcctg cgtcatccgt
     2401 cagcaggagc tggactttac tgatgcccgt tatatctgcg aaaagaccgg gatctggacc
     2461 cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga ttgaaccgcc tgacagtgtg
     2521 acctttcaca tctggacggc gtacagcccg ttcaccacct gggttcagat tgtcaaagac
     2581 tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct tcgtgaacac cacgctcggt
     2641 gagacatggg aagcgaagat cggcgaacgt ccggatgctg aagtgatggc agagcggaaa
     2701 gagc
//
LOCUS       DBFIFS010000347         2664 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.348, whole genome shotgun sequence.
ACCESSION   DBFIFS010000347 DBFIFS010000000
VERSION     DBFIFS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.348"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..782
                     /locus_tag="N7A07_004747"
     CDS             <1..782
                     /locus_tag="N7A07_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGU2479237.1"
                     /translation="VAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLC
                     DLAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKP
                     MVDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRL
                     LYDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(924..1184)
                     /locus_tag="N7A07_004748"
                     /pseudo
     CDS             complement(924..1184)
                     /locus_tag="N7A07_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(1201..1898)
                     /locus_tag="N7A07_004749"
     CDS             complement(join(1201..1649,1649..1898))
                     /locus_tag="N7A07_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HGU2479238.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(2136..2594)
                     /locus_tag="N7A07_004750"
     CDS             complement(2136..2594)
                     /locus_tag="N7A07_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479239.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          768 a          550 c          584 g          762 t
ORIGIN      
        1 tggttgctgc taaatataga attccttcag tgatgctggc tgttggattc gcgcatacat
       61 ctgcccatat tcagatgtta aaccgttctt taagcaatgc ttacaggcgg catggagtca
      121 gcggtccact atgtgattta gcatggattg atgttgctcc cccaagtatg agcattctta
      181 aaaatgctgg agaaccggtt atctcaatga gatatattcc ttataacgga ggtgctgtaa
      241 aggaaacatg gtgggacagg gattctgatc ggaaacgttt acttatcagc cttggcactg
      301 taaaaccaat ggttgatggt ctggagctga tttcatgggt tatggattct gcaaatgaag
      361 ttgatgctga tatcattttg caacttgcaa taaatgctcg tactggttta cgaaaactac
      421 catcaaatgt acgtctggtt gactggatac ctatgggtgt attccttaat ggagctgatg
      481 gatttattca tcacggtggc gcaggtaata ccctgacagc gctgtatagt ggaataccac
      541 agattgtgtt tggcgaaggt gcagatcgct ctgttaatgc agaaattgtt gcgaagcgtg
      601 ggtgtgggat tattccggac aagcatggac tgaccagtga tttggtaaat cgccttcttt
      661 atgatgattc actacgcttc tgttcagatc aggtagccgc tgaaatggct gaacaaccca
      721 gtcctgcaga gattgcagag gttttgatga gaaaattaaa aaacaacggg aaacaattgt
      781 agcgtattgc tgaaattttc aacgagaaat gttatgagta atataatgtc cttcatcaca
      841 taaatgaagg acatcactaa ataaatgaga tagtctccca taataatatt gcagaaagag
      901 ttatctgacg ataaaacgat attttacaca gagttattat ttgatacctt cctgcaatta
      961 taatttgcta attcatcaat aattgctccg cgccaccagg cataatcgtg accaccggta
     1021 tagacagtga ggttactttc caaaccagct gcgattaacg aatgatgcaa tcgctgaaca
     1081 tgtgagacgg ttgtaccttc taaagaaccg actccaagtc gaatatttac atctttcgga
     1141 ggcgctgaaa gtatctgctc acttacccag gaggtattat tctcaggtaa tgactccaac
     1201 ttactgatag tgttttatgt tcagataatg cccgatgacc ttgtcatgca gctccaccga
     1261 ttttgagaac gacagtgact tccgtcccag ccttgccaga tgttgtctca gattcagatt
     1321 atgtcgctca atgcgctgag tgtaacgctt gctgataacg tgcagctttc ccttcaggcg
     1381 tgattcatac agcggccagc catccgtcat ccataccacg acctcaaagg ccgacagcag
     1441 gctcagaaga cgctccagtg tggccagagt gcgttcaccg aagacgtgcg ccacaaccgt
     1501 cctccgtatc ctgtcatacg cgtaaaacag ccagcgctga cgtgatttag caccgacgta
     1561 gccccactgt tcgtccattt cagcgcagac aatcacatca ctgcccggtt gtatgcgcga
     1621 ggttaccgac tgcggcctga gttttttaag tgacgtaaaa ccgtgttgag gccaacgccc
     1681 ataatgcgtg cactggcgcg acatccgacg ccattcatgg ccatatcaat gattttctgg
     1741 tgcttaccgg gctgagaggc ggtgtaagtg aactgtagtt gccatgtttt acggcaagga
     1801 gagcagagat agcgctgatg tccggcagtg cttttgccgt tacgcaccac gccttcagta
     1861 gcggagcagg aaggacatct gatggaaatg gaagccacgc aagcacctta aaatcaccat
     1921 catacactaa atcagtaagt tggcagcatt accagaaaat gataaggtaa acattgaaaa
     1981 gcagccgact gatgactgtt ttttatagtt gcatttttat tttttgttat tttcatttat
     2041 attcccttac ttgtcgttta aaattcttct atataacagg aagaggtaac catagaatga
     2101 acgtttattt tcctgtatca gaacagccat aacttttatt ttatagttgt tatcagtgaa
     2161 tgaatgcaac ctaatagaac aatgaggaaa acaatggaga aaaagcgacg gaccggacgc
     2221 catagttcat attctcgtgt atcagcctca cgtatcaacc tttctgaaat ggtttcggtt
     2281 tcttttcctc gcaccagaat atgtctgtaa tatttcctca tgacatatac cgaaaactga
     2341 aaccagatta atatggatgg acacacaaca cataaacttc tgatgatatc acccgggaaa
     2401 gtatcttggg cgggtaaaag aaagtcatat actctggata aaatcatcat cgagataaat
     2461 gagacgccag cacctatcca gtattcgata tgagaagatg ctgcattata tgcgtcagta
     2521 aaatttttct ttcttaatga aggtggaagt gctgaaagtt caggaatacg ttttcttgtc
     2581 cagtatattt tcatatagta actcatataa aaagtgttct actttactat gatgatgacg
     2641 atttataaaa ggcttataat gact
//
LOCUS       DBFIFS010000348         2661 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.349, whole genome shotgun sequence.
ACCESSION   DBFIFS010000348 DBFIFS010000000
VERSION     DBFIFS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.349"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..264
                     /locus_tag="N7A07_004751"
     CDS             <1..264
                     /locus_tag="N7A07_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2479240.1"
                     /translation="LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISK
                     VAKVCGYASVSYFTYVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            complement(306..740)
                     /locus_tag="N7A07_004752"
                     /pseudo
     CDS             complement(306..740)
                     /locus_tag="N7A07_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1227..2408
                     /locus_tag="N7A07_004753"
     CDS             1227..2408
                     /locus_tag="N7A07_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2479241.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVNRPAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            2412..>2661
                     /locus_tag="N7A07_004754"
     CDS             2412..>2661
                     /locus_tag="N7A07_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479242.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQCKLYKKSLGFSDVVVEF"
BASE COUNT          723 a          537 c          640 g          761 t
ORIGIN      
        1 ttatatctca gcgaaagttt actaaagagg aaattgaaag aagagggggt atcattcagt
       61 aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac tcgttgttat
      121 cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt tacttatgta
      181 tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc agaaagtaaa
      241 attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca gacagggggg
      301 gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg ataacccaac
      361 gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt atgcaatgcc
      421 gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt tttcacgaac
      481 ctttctgcaa ttccattact ctgaggactg ctgattgccg ttgtgcaggg ctccagattc
      541 aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt atctgtcagc
      601 cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc tcctaacatg
      661 acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc tatggcttca
      721 cgatcgcaac agtccagggc gattcatctg ggccaatcct tgctcataac aggcattcac
      781 ttcagtcatg gcagaaaggt atgcatgctg gagaagtcgt gaaagaaaag aagactgctg
      841 cgccgtttgt cgtcacgttt atcttcattg gctatgcaag tcgtaataca aggtgggaca
      901 aaactgagac acataaggcc tcgcaatggc ttgcaaggct ttacatgttt tgatgtggtg
      961 ggacgtgtga gcgcagtgtt gatggggtaa tgctttgaat tagaagcgga ttcttataat
     1021 tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc accatctcaa cttcctcaaa
     1081 cgtccgtatt agtccataaa atctctgatt tataacattt tttattcttt taagtccata
     1141 gtagtccgta accatccagt agaatccggt actgaatgtg tataggattg tgtatatgtt
     1201 cctgttcggt ctggattcct atacacatgc ctttaaacga tatgcagatt cgccgcgcta
     1261 agcctgaagc taaagcctat acatttggag atgggctagg gttgtcatta cttatagaac
     1321 ctaatggaag caagagttgg cggttccgct atcgctatgc cggcaaaccc aaaatgatct
     1381 cgcttggtgt ttacccaacg atcacccttg ccgatgctcg ttcccgtcgt gatgaagctc
     1441 gaaaacttgt ggcagaagga aagaacccta gtgaggttcg aaaagagcaa aagctagcta
     1501 tgcaaacaga gtcagagaac gccttcgaaa agatagccag agagtggcat caacttaaat
     1561 ctgctaaatg gtcggcggga tatgcatcag acatcatgga agcgtttaag aacgacattt
     1621 ttccttatgt cggaacaagg cctgtgggag agattaaacc gctagagctg ctgaacgttc
     1681 tgcgtaaaat tgagaaacgt ggtgcgttgg agaaaatgcg caaagtgcgg cagcgttgct
     1741 ccgaagtgaa ccgccccgca attgcaacgg gtagggcgga gtacaatcct gcggctgatc
     1801 tctccagcgc tctcgaagta caccaatcca atcatttccc attcctaaaa gctgatgaga
     1861 tacctgattt tctacgtgcc ttagagggtt actccgggag taagcttgtc cagatagcca
     1921 cgaaattact gatgattacg ggtgtgagaa ccatcgaatt acgcgcggca ttatggcaag
     1981 aatttgatct ggataacgct atttgggaaa ttcctgctga aaggatgaaa atgcgtaggc
     2041 cacatcttgt gcccttatca tctcaagcgg tagatttact caatgaactc aagatcatga
     2101 cagggaacta tcgttatgtt tttccagggc ggaacgatcc gaataggcca atgagcgaag
     2161 cgagtataaa tcaagccatt aagcgtattg ggtatggagg aaaagtcact ggacatggtt
     2221 ttcgtcatac cctttctaca atcctgcatg agcaaggttt tgagagtgct tggattgaaa
     2281 tccagttggc tcatgtagat aaaaattcta ttagggggac ttataaccat gctcaatatt
     2341 ttagtggaag gaagtctatg atggactggt acagtaattt gatatttgaa agactaaaaa
     2401 ggagttaatt tgtgtctcaa ccactttcgg aaatattgac atgggatgat gaacaatggg
     2461 aggtatttgt ccatgattgg cttattgtct gtaaatcaga tgattacccg tggagcgaac
     2521 gtttgggagg agctggagat aaaggtagag acgttgttgg atataaatcg gatcctaacg
     2581 tagaaggtta ttcttgggat aattatcaat gcaaactgta caaaaaaagt ttagggttct
     2641 ctgatgttgt agttgagttt g
//
LOCUS       DBFIFS010000349         2650 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.350, whole genome shotgun sequence.
ACCESSION   DBFIFS010000349 DBFIFS010000000
VERSION     DBFIFS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.350"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..508
                     /locus_tag="N7A07_004755"
                     /pseudo
     CDS             1..508
                     /locus_tag="N7A07_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            complement(525..845)
                     /locus_tag="N7A07_004756"
     CDS             complement(525..845)
                     /locus_tag="N7A07_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2479243.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            953..1126
                     /locus_tag="N7A07_004757"
     CDS             953..1126
                     /locus_tag="N7A07_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2479244.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            1197..2120
                     /locus_tag="N7A07_004758"
     CDS             1197..2120
                     /locus_tag="N7A07_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2479245.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTR
                     DVDINEPDGPTNLSAWVRLKNKELPPSLHRY"
     gene            2175..>2650
                     /locus_tag="N7A07_004759"
     CDS             2175..>2650
                     /locus_tag="N7A07_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2479246.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTG"
BASE COUNT          662 a          600 c          708 g          680 t
ORIGIN      
        1 agtgcccgcc cgtcgctgac ggtgtccaat ctgttcggtc ttgtcaccgg gatggcggag
       61 gatttgcaga gcctggtggg tgccacggtg gtccgccgcc gggtgtatgc ccgttttctg
      121 gatgcggtga attttgtggc gggcggtcgt ggttatcggg cggcagaggt aaaaagaata
      181 aaaaaatccc gcagagttgc ggggacagac aaagattaac gttaagatgt catcgctgac
      241 ggccatgacg gcctcgtttg tgctggccac accgaccgag acggacgggg cgctgtttcc
      301 cggtcgcatc atgctggcga acacctgtat gtgggattac cggggagatg aatgcgggta
      361 taacggtcct gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag
      421 atgcagcaag tgcatgcgcg ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt
      481 cctttccatt aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta
      541 tttagtagtt ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc
      601 atggcgataa cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg
      661 ctgttaattt tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt
      721 atctgaacaa tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca
      781 tctcgcattc cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg
      841 gccattgcaa atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatggtg
      901 gcattatggc cttcatggcg tcactttggc accaaataaa ggagattaga taatgcaaga
      961 tacacttttc actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa
     1021 tgaagacctt cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg
     1081 tttgctggca aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct
     1141 caatggctgc aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga
     1201 ctagtttagc aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt
     1261 tggttggaat taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga
     1321 tggtttgggc tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa
     1381 tcgaaatacc ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg
     1441 cggcttggct ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa
     1501 tccagtatca ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga
     1561 ttaacccgcg caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag
     1621 ccattaaaca actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg
     1681 cgatgatcac catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca
     1741 gcgaggagca gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt
     1801 taatcggcaa acaagagaag agcaccaacg agctttccgc aaaagaagca aacagccttg
     1861 tatggttatg ggattatgct aaccgctcac aggcattatt ccgcgaactg tatccggcgc
     1921 taaaacaaat tcaatcgaac tattccggca gatgctacga ctacggtcat gagttttcgt
     1981 atgttatcgg aatggcgaga gatgttttaa tcaaccacac acgagatgtt gatattaatg
     2041 agccagacgg accaacgaac ctttccgcat gggtaaggct taagaacaaa gagttgccgc
     2101 cttcactgca tcgctactga caattgacaa cttaacaaac ccagcttcgg ctgggttttt
     2161 tatcaggagt tctcatgctc tatagcaata tattggcgca cgcccggcga tgtgcgccag
     2221 cggagtcgtg cggcttcgtg gtgagaacgc cggaggggga gcggtatatc ccttgtgtga
     2281 atatctctgc agagccggag gcgtattttc gtattgcacc ggaagactgg ctgcgggcag
     2341 agatgcaggg ggagattgtg gcactggtcc acagtcatcc cggtggtctg ccctggctga
     2401 gcgaggccga ccggcggctg cagataaaaa gtgcactgtc ctggtggctg gtctgccggg
     2461 gggacattca taaattccgc tgtgtgccac atctgacagg acggcgcttt gagcacgggg
     2521 tgacggactg ttacacgctg ttccgggatg cataccatct ggcgggaatt gatatgccgg
     2581 attttgagcg tgaggatgac tggtggcgta acggtcagaa cctttacctg gacaatatgg
     2641 cggtcaccgg
//
LOCUS       DBFIFS010000350         2631 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.351, whole genome shotgun sequence.
ACCESSION   DBFIFS010000350 DBFIFS010000000
VERSION     DBFIFS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.351"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..572)
                     /locus_tag="N7A07_004760"
     CDS             complement(<1..572)
                     /locus_tag="N7A07_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU2479247.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
     gene            complement(668..856)
                     /locus_tag="N7A07_004761"
     CDS             complement(668..856)
                     /locus_tag="N7A07_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2479248.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(853..1041)
                     /gene="dicB"
                     /locus_tag="N7A07_004762"
     CDS             complement(853..1041)
                     /gene="dicB"
                     /locus_tag="N7A07_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2479249.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(1522..1674)
                     /locus_tag="N7A07_004763"
     CDS             complement(1522..1674)
                     /locus_tag="N7A07_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2479250.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(1949..2593)
                     /locus_tag="N7A07_004764"
     CDS             complement(1949..2593)
                     /locus_tag="N7A07_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2479251.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
BASE COUNT          677 a          612 c          579 g          763 t
ORIGIN      
        1 tctgcctgaa tttcaggcca tttagcgcca ggctcacatt tatgcttaac ccacccgatg
       61 gcatgcagtt taagttcagg atacatggcg ttaacttctg gcattttcat caacgcttca
      121 acgatatgtc cgccgaatgt tgccatgtct tcctgtagta attcctgtgc gctaatcacc
      181 atatcaacgg tgatgttttc gcatgtgtcg aatttaacca ggactgcgtt ctgtacttca
      241 ggggacagct tgtcaaaatt gacgttcatc ggatcggatt ctggttcgac cggtacaaag
      301 gaagctgact cctcatccca gcggttttcc tgcatatatt cggtatccca ggaatcgagg
      361 gcagggcggg gtatgccggg tttatcctcg cagacaataa atttataagc gcagtcctga
      421 gctgccggga attgctccag aaattgccag tgaaattttg cgcgtgcgcg acgctcatca
      481 ccggcttcaa tggcagtggc caccgcaaca gcgctatctt cttttatggc ctgttcgtca
      541 ggaatagcag cgcaaataaa gattttactc attgtgtttt aacctcatta cagatttcag
      601 ggtgaacgaa tccctgccat tgctggcatt tttaatccgt tggtatggcg ttaatatggc
      661 tggcgggtta tccagccggt gtttcgttat tcaggtacag cgatactttt tttaccggta
      721 ggcattcacc agaaattttt tgctcgtctc ttgcctggag gcaggattct ttactggcat
      781 aaattccggt aataacattc tgtgattcac ccgttataag aaaaaccgtc atcaccagtg
      841 caaatgctga agtcattgac gttctccgaa aataccaagt ttaataaggg caattcggga
      901 aagaatggaa ttatcattga gcagataagg ctcatatttc cgcatattaa tggcatcctc
      961 agtaaacgct ttattactga gcagaacacc aatatcaaaa caaccttcag acgtattaac
     1021 gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg aattttgtga tgcagtgcct
     1081 ggtgcctcca ggtgacgtta accagttaac aattaacgtc gggttagttg atgctcgtta
     1141 cgccagtaaa agaccgcctt actgctttaa ctgttccgcg tgcgcatagc cgcattcacc
     1201 gcatcacaaa attcacttta aaaagggcgg atatccattt ccgccgaatc accagaaaag
     1261 tgataacaga ggttgttgtg gctggggtgt cacttaagcg tatggtcaac ctgacaaccc
     1321 ggtgtcctca acgggggaag gaataacccc gccatactta ccgccgcgcc atttcgcgga
     1381 ttgccacaac cagaagcgca cggtcgaatt aaatttaacg acgacctata cagagagact
     1441 aacttcgccg tgcgctttcg tgttatgccc tgacttttca gggtaaatta acctgtggaa
     1501 acctgttttt actggcgcta atcagttagc gtttcttgct aaccagcgat gcgcgccagc
     1561 ttcggtttta aacgttttac ttctggtata cgtcatcgcg gtaaacgtgc cgtcgtggtt
     1621 ggggaatact ccgcacacca gagattcatt gttgccaaga ttgagcatat ccatgttgac
     1681 ctcatttacc cttaacgccg ggtcgcggaa ctaaaaacct gctgcgctgt tatacaaagt
     1741 gttcccgccg tcatgttcat acgcctcggg ctggctactt aacccctgac cactgccggg
     1801 taactcgagg tattgcccga cgttctgtgg ggcgaggtag ggtggtatgt aataaatcta
     1861 aaagtattta gtttttcagt caaggtaaat ctagattaat ttaaaaaaaa ggtcggcatt
     1921 gccgacctca agtaaaggac gtggcgggtt ataagttgaa ctgtacacct tttgctacgg
     1981 caacaatttt acactctggt gtaagtaagg atgactgata gcgtggattg agagggctta
     2041 aatacacaag cttcccatca ataactaatt tctttatagt catagatggt tcatttttaa
     2101 gcggatccgg aactattaca gcgacgatgc tgccattttt ataactttct cctggcctta
     2161 ggatcacagt agcaccaact gggatactcg gtgatcctga tgggttatgc attgtgtcat
     2221 caggcattga aacggcaaaa tcaccttcct cgacatcaaa gaatgtggtg attctatcga
     2281 catttcccat tgttttctct ccttttaaga ttaggaaaga aatcgcctca ccccacgaaa
     2341 ggtaggggat cttggtgcct gaggtacttt gtacaaaaga taattcagga gacgatactc
     2401 catataggag ataggactca gtagttccta atgcctgggc taacttactt agagctttgc
     2461 tacctggttc gtttagatct ttctcccagt accctatagt aaccccagtc acgcctgaaa
     2521 gcttacccag ttctacttgg gtcagcccct tatcttttct aagtctctta agcctgaagc
     2581 caaggctttc catcattttc tcctgcaaat tgaatataaa ttattttaga t
//
LOCUS       DBFIFS010000351         2627 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.352, whole genome shotgun sequence.
ACCESSION   DBFIFS010000351 DBFIFS010000000
VERSION     DBFIFS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.352"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            229..645
                     /locus_tag="N7A07_004765"
     CDS             229..645
                     /locus_tag="N7A07_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU2479252.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSIR
                     QNARLHAMLSEISKKATYHGKARNIEFWKGLFVSGWQIATNQHPEIISGLEGELINIR
                     ESTATLSVKKISEIMDYIEAYCAMNSIHLSEWRNYD"
     gene            638..1240
                     /locus_tag="N7A07_004766"
     CDS             638..1240
                     /locus_tag="N7A07_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HGU2479253.1"
                     /translation="MIEVWVDIEGIPFYQVSNKGNFRSITREVTVTSTRQRPYKKIIN
                     GTSVKPFKCKSTGYLQIKVYGKKYSAHRIVAKAFCTGFCDGLVVNHKNGRRDDNRADN
                     LEWVSHSENSKHGYKQNGRIPISLGKFSGDHPASKAVISTDMKTGEEVYYEAAMDAVR
                     EGFDSSSISRCCNGESSYHKGRFWRFANEKMKARWGDRAA"
     gene            1237..1764
                     /locus_tag="N7A07_004767"
     CDS             1237..1764
                     /locus_tag="N7A07_004767"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU2479254.1"
                     /translation="MTIKSNTPAHNKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEVDDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1761..1955
                     /locus_tag="N7A07_004768"
     CDS             1761..1955
                     /locus_tag="N7A07_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2479255.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTRGLTR"
     gene            2212..>2627
                     /locus_tag="N7A07_004769"
     CDS             2212..>2627
                     /locus_tag="N7A07_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU2479256.1"
                     /translation="MATKIAVETLSPITHNQIPVITTELLAHLYGTDVANIKMNHSRN
                     QTRFLEGKHYFKIVGDDLKNLRVTFSYLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQCKKNTGKQEKKPNGLSAKETDSLV"
BASE COUNT          825 a          557 c          645 g          600 t
ORIGIN      
        1 actcaataat gagtttcagc gcaacagaat tatccggagt ctggatttaa agtgaaggag
       61 tgagcatgag cgagcaaata ttcagagaga ttaagccacg gttttatcgc aaggtaaggg
      121 tggtttatca ggacgaaaac aagacatgtg catacgccat tcataatggt cggtggtcag
      181 tgttcgacac caaaaacttc gagaagaact tcgagaggat taagggtgat gaggaaacta
      241 acatttgaac taagaagccc catccatcag cagaacgcca ttcaagctat ccagcaaatt
      301 cttccagacc caaccaaacc aatcgtagta accattcagg aacgcaaccg cagcattcgg
      361 caaaatgcac gccttcacgc gatgctatct gaaataagta agaaggctac atatcacgga
      421 aaagcaagaa atattgagtt ttggaagggg ttattcgttt ctggttggca gattgcaacc
      481 aaccagcacc ctgagattat atcagggtta gaaggtgagc taataaacat cagagagagt
      541 acggcgactc tatctgtaaa aaaaatatcc gaaataatgg actacataga agcatattgt
      601 gccatgaact caattcatct tagcgaatgg aggaattatg attgaggttt gggtagatat
      661 cgaagggatt ccattttatc aggttagcaa taaaggaaat ttcaggtcta ttacgaggga
      721 agttacagta acatcaacca gacagaggcc atataagaaa ataattaatg gcactagtgt
      781 aaaaccattc aagtgcaagt cgacaggata tcttcaaata aaggtatacg gtaagaaata
      841 cagcgcccac aggatagttg cgaaagcatt ctgtacaggg ttctgtgatg gcttggtagt
      901 taatcacaaa aatgggcgaa gagatgacaa tagggctgat aaccttgaat gggtatcaca
      961 ttctgaaaac tcaaaacacg gatataaaca aaatggaaga atacctatat cgctaggtaa
     1021 atttagtggt gaccatcctg ccagtaaagc tgttatttct actgacatga aaactgggga
     1081 ggaggtttat tatgaagcag ctatggatgc tgtcagagaa ggatttgata gttcgtcaat
     1141 tagtcgttgc tgtaatggcg aaagctcata tcacaaagga agattctggc gatttgcaaa
     1201 tgaaaaaatg aaagcgcgat ggggagatcg ggctgcatga ctatcaaatc aaatacgcca
     1261 gcacacaaca aggactgctg gcaaacgccg ctttggcttt ttgatgcact ggatattgag
     1321 tttggattct ggctggattc agctgcgagc gacaaaaatg ctctgtgcgc tcactggcta
     1381 actgaggtcg acgacgcgct caattctgag tgggtaagcc acggtgcaat ctggaataac
     1441 ccaccgtaca gcaatatcag gccgtgggtg gaaaaagccg ctgagcagtg catacaacag
     1501 cgacagacgg tagttatgct tgtgccagag gatatgtcag tcggatggtt cagcaaggct
     1561 ctggagagtg ttgacgaagt tcgcattatc actgatggac ggattaattt tatcgaacca
     1621 tcgacggggc tggagaagaa gggaaacagt aaaggctcca tgctgctgat ttggcgaccg
     1681 ttcatcagtc ctcgacggat gtttactacc gtatccaaag cggcattgat ggcgatcggg
     1741 cagggcgtca ggagggcggc atgaggcgac agcgacgaag tatcaccgac atcatctgcg
     1801 aaaactgcaa ataccttcca acgaaacgct ccagaaataa acgcaagcca atcccaaaag
     1861 aatctgacgt aaaaaccttc aattacacgg ctcacctgtg ggatatccgg tggctaagac
     1921 atcgtgcgag gaatacaagg ggattgacgc gatgatttat ccggggctat attcctcaca
     1981 cgccagcaaa atctggcgtc gggattggcg tcccggatga aaaaggcgac aacagacgcg
     2041 ccagcgtctt ttttattgtc gtttgcacag tcacatctca atggtgggct gtgtgggggc
     2101 ggagcaatcc gcgccggttc ctttttcccg gttacgccaa ccctgcacag ttcaccacca
     2161 agcgattggc gtcgcaggtg gtgatgattc acaaagaaaa aggatcatct tatggccacc
     2221 aaaatcgcag ttgaaactct ttccccgatt acccacaacc agatccctgt cataaccacc
     2281 gagctattgg cgcatcttta tggaacggat gttgccaaca taaaaatgaa tcattcacgt
     2341 aatcaaactc gttttctgga agggaagcat tatttcaaaa tcgttggcga tgatctgaaa
     2401 aatttgcgag taacttttag ttacctgcaa atttccccca aaacccgctc cctcatcctc
     2461 tggactgaac gcggcgctgc ccgtcacgcc aaaatgctcg aaaccgatca ggcgtgggaa
     2521 gtgttcgaaa aactggaaga ctgctatttc agccagtgca agaaaaatac tggcaaacaa
     2581 gagaagaagc ccaacgggct ttccgcaaaa gaaacagaca gccttgt
//
LOCUS       DBFIFS010000352         2626 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.353, whole genome shotgun sequence.
ACCESSION   DBFIFS010000352 DBFIFS010000000
VERSION     DBFIFS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.353"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..459
                     /gene="yciF"
                     /locus_tag="N7A07_004770"
     CDS             <1..459
                     /gene="yciF"
                     /locus_tag="N7A07_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HGU2479257.1"
                     /translation="SDTYSAEKQLTRALAKLARATSNEKLSQAFHAHLEETHGQIERI
                     DQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAALIAAAQKVEHYEIAS
                     YGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNKKAENKA"
     gene            505..1011
                     /gene="yciE"
                     /locus_tag="N7A07_004771"
     CDS             505..1011
                     /gene="yciE"
                     /locus_tag="N7A07_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2479258.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRSRIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1071..1196)
                     /locus_tag="N7A07_004772"
                     /pseudo
     CDS             complement(1071..1196)
                     /locus_tag="N7A07_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            1420..1521
                     /locus_tag="N7A07_004773"
                     /pseudo
     CDS             1420..1521
                     /locus_tag="N7A07_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1931..2166)
                     /locus_tag="N7A07_004774"
                     /pseudo
     CDS             complement(1931..2166)
                     /locus_tag="N7A07_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2325..>2626)
                     /locus_tag="N7A07_004775"
     CDS             complement(2325..>2626)
                     /locus_tag="N7A07_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2479259.1"
                     /translation="RLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSRV
                     CSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          828 a          570 c          503 g          725 t
ORIGIN      
        1 tcagatacct acagcgcaga aaaacaatta acccgggcac tggcaaaact cgcaagagca
       61 acatcaaacg aaaaattaag tcaggctttt catgcgcacc tcgaggaaac acatggacag
      121 attgaacgta ttgatcaagt tgtggaatca gaatcgaatc tgaaaattaa gcgcatgaaa
      181 tgtgtggcaa tggaaggtct tattgaagaa gctaatgagg tcatcgaaag taccgagaaa
      241 aacgaagtgc gtgatgccgc actgattgcc gcagcacaga aagtcgagca ttatgagatt
      301 gccagttacg ggacattagc gacgctggct gaacaattag gttaccgtaa agcagcgaag
      361 cttctgaaag agaccctgga agaagaaaag gccaccgaca tcaaactgac tgatctggcc
      421 cttaataacg taaataagaa agccgaaaat aaagcctgaa atatgaactt taacttttgg
      481 tcattttata aagaggacat tttcatgaat cgtattgaac attatcatga ctggttacgt
      541 gacgcccatg caatggaaaa gcaagccgaa tctatgcttg aatccatggc cagccgtata
      601 gataattatc ctgaactacg ttctcgtatt gaacaacatc ttagtgaaac caaaaaccag
      661 attgttcaac tggaaactat tcttgatcgt aatgacattt cacgttcagt cattaaagat
      721 tccatgagta aaatggctgc gcttgggcag tcaatcggtg gtatattccc ttctgatgaa
      781 atagtcaaag gctctattag cggatatgtc ttcgagcaat ttgaaatcgc ctgttacacc
      841 tctctattag cagcagcaaa aaatgccggt gatacagcct caattccgat catcgaagcg
      901 attttaaatg aggaaaagca aatggccgac tggctgattc agcatattcc gcaaacaact
      961 gagaaatttt taattcgctc tgaaactgat ggcgtagaag cgaagaaata atacccttta
     1021 tgccatgtcc ttattgaccc cgtatattac ggggtcgttt ttgtgcggaa ttaaaaacga
     1081 tatcctgctg agaacataaa cacccacgga tcgaggcgta cgctatcatg ttgctgtgca
     1141 ccgcccagtt tataattggc ggtggtatcg atatccatgt accacactgg tagattttac
     1201 aaaactcaat aacatattat atttaaagga aaaatacaga atttacgatg cttttttata
     1261 cagatctctc gtgtttttaa ctgattgatt ttgttgaact ctttttttgt tttgcaggag
     1321 ttactcctct taccttattg tccactcgat gaccaacaca ttcacaaccc aaaccagaat
     1381 atcctatcta tgaagcatgt atccgcacgc agattaatta tggtttacat tgttctacca
     1441 caatagtcgc atcctaaatg ggtgcggttg aggatcacca tccttgaaga ggatgcccgc
     1501 cactttaact gcatgacagg gctatttaaa caatcactaa aaatcgccca agagcattct
     1561 gtttctataa tgtattccaa gagattaaaa aataatatca tacgttattc ggcagagttc
     1621 gtatgtacta cggcgtccca accatgtccc aaaacaaaaa tttctgccct atacacgttc
     1681 caaaaaattc aacagcaaag agagtctgct gacgagttgt tgattcactg attaatacgt
     1741 aaaattgata actgctggaa atcatacaat actcgtactt tcgaaagttc gctaaccagg
     1801 tgctacacgc gaggtgttgc agttttcttt acgatttatc attcaatcct aatccatgca
     1861 tgacatgtgg tgcctgtggg gtgatcctac ctacgtaatg tggacacagc cctaatcaag
     1921 gttctgtttt tcaaaacagt tccgtgctga gacagccaca agtactgtgt cggcaccacc
     1981 gattgtaatc acactagatc taattaaaca cagccgtctg cattatttcc cgactcataa
     2041 aatattagcc gtaaatgcat tcgactttta gtgagtggaa gaaactttcc gccctagcat
     2101 tatcgtaaca gcgccccttt gcactcatcc tgtcatgcag gtatgccgct tcagaagcgc
     2161 ctgataatgc gttgaaatca ggacggtggc tacttgctat tcagttcacg atacatccct
     2221 taaggaccag cgaatagcaa tgagtcactt tattatcaag tttgatgacc gctttgatgc
     2281 taacttttaa taagagtctc ttcacacaaa atcgtgtacc gagtctacac tgaataacaa
     2341 tcactcctca ccgtaaagtg accgatactg caatcaaact cacactgctc tctggccaca
     2401 agcattgata atgttattgg ctctctgctt agtgggtgat gcatcccatc attaattata
     2461 cgagaaagag ccatttcatc atataaacta catactctgg aaccttcagc atttcttaaa
     2521 aaaacacctc gttgaggaat tgccagcgta atcgggcact gtaatgcttc atctatgtaa
     2581 gacatttctt caatattaac taaaaatgaa catgaatgta atcgcc
//
LOCUS       DBFIFS010000353         2598 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.354, whole genome shotgun sequence.
ACCESSION   DBFIFS010000353 DBFIFS010000000
VERSION     DBFIFS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.354"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            80..1081
                     /gene="gap"
                     /locus_tag="N7A07_004776"
     CDS             80..1081
                     /gene="gap"
                     /locus_tag="N7A07_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU2479260.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1123..2562)
                     /gene="aldA"
                     /locus_tag="N7A07_004777"
     CDS             complement(1123..2562)
                     /gene="aldA"
                     /locus_tag="N7A07_004777"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU2479261.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
BASE COUNT          651 a          688 c          606 g          653 t
ORIGIN      
        1 gagattgccc atcccgccat cctggtctaa gcctggaaag gatcaatttt catccgaacg
       61 ttcctgacag gagaaaacca tgagtaaagt tggtattaac ggttttggtc gtatcggtcg
      121 actggtgttg cgtcgattac ttgaagtcaa aagcaacata gacgttgtcg ctattaatga
      181 tcttacttcc ccaaaaattc tcgcctatct gctgaaacat gattcaaact atggtccgtt
      241 cccctggagc gttgatttta cggaagattc acttatcgtt gatgggaaaa gtatcgcggt
      301 ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg aaaggtgcag aaatcattgt
      361 cgaatgtact ggcttttata cctccgccga gaaatcgcag gcgcatcttg atgctggcgc
      421 gaagaaggtg ttgatttccg cccctgcagg tgaaatgaaa actatcgttt ataaagtcaa
      481 tgacgacact ctggatggca acgacaccat tgtttccgtg gcatcatgca ctactaactg
      541 tcttgcgccg atggccaaag ccttgcatga cagtttcggg atagaagtcg gcacgatgac
      601 gaccattcat gcctatactg gcacccagtc actggtggat ggcccgcgtg gtaaagattt
      661 acgtgcttca cgcgccgcgg cagaaaatat cattccccac actacgggcg cggcaaaagc
      721 cattggtctg gtgatcccgg aactgagcgg caaactgaaa ggtcatgcgc aacgcgtgcc
      781 ggtgaaaaca ggttcggtca ctgaactggt atcgattctc ggaaaaaaag tgactgccga
      841 agaggtgaat aacgcactta aacaagcaac caccaataac gagtcatttg gttataccga
      901 tgaagaaata gtctcttccg atatcattgg cagccatttc ggttcggtgt ttgatgccac
      961 gcaaacggaa attaccgccg tgggcgattt acaactggtg aaaacggtcg cctggtacga
     1021 caacgaatat ggcttcgtca cgcagcttat tcgcaccctc gaaaaattcg ctaaactctg
     1081 acgcgcacag gcggaggaaa aaacctccgc ctctttcact cattaagact gtaaataaac
     1141 cacctgggtc tgcagatatt catgcaggcc atgtttacca tctgcgccgc caataccgga
     1201 tttacgccat ccggcgtgga agccttgcat agcttcgaag ttttcacggt tgatgtaagt
     1261 ttcaccaaac ttcagccctt taatggcttt catcgcgacg ttcagatttt gggtatagat
     1321 tgatgaggtc aggccgtaat cactgtcatt agccattgag atagcatctt ccagcgtgtc
     1381 aaatgcgact accggcagaa ccgggccaaa ggtttcctca tgcataatcg acatttcctg
     1441 gcgaacatcc agcagcaatg tcggcggata ataatatcct ttcccctcta ccgctttgcc
     1501 acccaacgcc actctcgccc cttcttctac tgcgcgcgcc actttttgct cgaccctttc
     1561 cagcgccgcg gcgttaatca acggccccat cgcaatgtcg ttgcgttcag cggggttacc
     1621 aaattgaacc gcctgcatcg cttcacccag ccgattgacg aactgatcat aaatgccttt
     1681 ctgtacataa acacgttctg cacagttaca cacttgccca ctattaatga cgcgtgaatc
     1741 aacgatggct ttgactgcca gttcaagatc ggcatcgtcc attacgatag ctggtgcttt
     1801 accccccagt tccaggcaca ctttggtgat gtttttcgcc gcagtcgcca tgatcttctc
     1861 acctgcagag acgctgcctg tcatactgac cattgcgacc tttgggttac ccgccagttc
     1921 ttgcccaacg gtttcaccac gccccagaac aaggttaaac acgccgcgcg gaaggcctat
     1981 ttcatcgacg attttggcga atgcaatcgc attgtttggc gtaaattcac tgggtttaat
     2041 gacaatggta ttaccagtca aaagagcggg agccattttg cgggcaatga ggaagaacgg
     2101 gaagttccac ggcagaatgc cggtagtcac accaagcgca cgtttaaaca gaagaatatt
     2161 ttctcctgga cgatcgcttt gaataatctc gccctcgtaa cgccgtgccc actccgccat
     2221 gtaatcgata tagtcggcag taaaagccac ttcgacttca gccagctgct ggatcttgcc
     2281 cccttcttca acaatcagcg cactgatttc actggcgcgt tcgcggatcc cggcggagat
     2341 tttgcgcaac caactggcgc gttcaattgc aggcaacgct tcccattctg gttgtgcacg
     2401 ttctgctgca tcgattgcct tacgggcatc ctcggcctga ccatcgggaa tgcgggaaat
     2461 gacagcctct gtagcagggt ttaccacatc aatccatgcg tctccacgcc aggtaacaaa
     2521 ctgtccatca atatacatag gatgttgaac gggtactgac atgggcgact cctgtgattt
     2581 atatgtttgt ttttggca
//
LOCUS       DBFIFS010000354         2571 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.355, whole genome shotgun sequence.
ACCESSION   DBFIFS010000354 DBFIFS010000000
VERSION     DBFIFS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.355"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1676
                     /gene="espP"
                     /locus_tag="N7A07_004778"
     CDS             <1..1676
                     /gene="espP"
                     /locus_tag="N7A07_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU2479262.1"
                     /translation="VTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLL
                     TDDSARLVAKNKASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVS
                     YRGSVNAPSASATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELAT
                     SNSAYAMRTNLSESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNEN
                     VFKASKQTIGFSDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFL
                     NEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLD
                     LFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTAT
                     FAGLGTRDYSTHSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLS
                     MKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIK
                     GEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(1932..2413)
                     /locus_tag="N7A07_004779"
                     /pseudo
     CDS             complement(1932..2413)
                     /locus_tag="N7A07_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          699 a          609 c          629 g          634 t
ORIGIN      
        1 atgtgacaac tcaaagtggt atgttctcca cgagcgatat cagcatctct ggtaatctgt
       61 ccatgacagg caatcccgac aaagacaata aattcgagcc ctcaatatat ctgaatgatg
      121 cttcttatct actgactgac gactccgcca gactcgttgc caaaaataaa gcatctgtgg
      181 tgggagatat acactccact aaaagtgcat ccatcatgtt tggtcatgat gaaagcgacc
      241 tctcgcagtt gtctgacaga acctcaaaag ggcttgcact tggtctttta ggtggctttg
      301 atgtctcata tcgcggttca gtcaatgccc cgtcagcatc tgccactatg aacaacacct
      361 ggtggcaact aaccggagat tctgcgctga aaacactgaa aagtacaaac agcatggtct
      421 atttcactga cagcgcaaac aataagaaat tccatacgct gacggtcgat gagctggcaa
      481 ccagcaacag cgcctatgcg atgcgtacaa acctttctga atcagacaaa ctggaggtca
      541 aaaaacactt gtctggtgag aacaatattt tactcgttga tttccttcag aaaccaacgc
      601 ctgaaaaaca actgaatatt gaactggtaa gcgcgccaaa agacaccaat gaaaatgtct
      661 ttaaagccag taaacaaacc attggtttca gtgatgtaac gccggtcatt acaaccaggg
      721 aaaccgatga caaaataaca tggtcactga caggctataa cacggtagca aacaaggaag
      781 caacccggaa tgccgccgcc ctgttctctg ttgactataa agcgtttctg aacgaggtca
      841 acaacctgaa caaacgtatg ggtgacctgc gtgatatcaa cggcgaagcc ggtgcatggg
      901 cacgcatcat gagcggtacc ggctctgcca gtggtggttt cagtgacaac tacacgcacg
      961 ttcaggtcgg ggtcgacaaa aaacacgagc tggacggact ggatttgttt accggtttca
     1021 ctgtcacaca cactgacagc agtgcctccg ccgatgtttt cagtggtaaa acgaagtctg
     1081 tgggggctgg cctgtatgct tccgccatgt ttgattccgg tgcctatatc gacctgattg
     1141 gcaagtatgt tcaccatgat aatgagtaca ctgcaacctt tgccggactc ggaacccgtg
     1201 attacagcac gcattcatgg tatgccggtg cagaagcggg ctaccgctat catgtcactg
     1261 aggatgcctg gattgagcca caggctgagc tggtttacgg ttctgtatcc ggtaaacagt
     1321 ttgcatggaa ggaccaggga atgcatctgt ccatgaagga caaggactac aatccgctga
     1381 ttggccgaac gggtgtggat gtgggtaaat ccttctctgg taaggactgg aaagtgacag
     1441 cccgtgccgg tctgggctac cagttcgacc tgctggctaa cggcgaaacc gtattgcggg
     1501 atgcatctgg tgaaaaacgc atcaaaggtg aaaaggacag ccgtatgctg atgtccgttg
     1561 gcctgaatgc agaaatcagg gataacgtcc gctttggact ggagtttgag aaatccgcct
     1621 ttggtaagta caacgttgat aatgctgtca acgctaattt ccgttactcg ttctgatact
     1681 ccgtattcaa aagggcctgc gggcccttta cgctcagtgc ctgccgccct gatatgtctg
     1741 agagacattt ccgggatgtc agtcgactgg tttacttaac tgcgatgact aaagctggca
     1801 gtaatgccgg cttttttacg gccacatacg tcttcctctc atccggaact gtgatacaaa
     1861 tcctgcatac aggattgacc gccggttcct gcaaatggca cattttcagt aagatagcga
     1921 aggttacatt tgtcactggt taacataccg atggtaaaag gggcaagcag cgccagcaat
     1981 tcatcagccc gggggccaaa agtgtaggcc agaacgccac cagttttcgt gttgtacgcg
     2041 taccagagcc tgtgctgtca cgctttactg ccgataaaac tccattgctc atcaagttcg
     2101 cagatgagtg ccatatcagc atgagcgacc ggggatgacg ttattcttcg tggcgagcgt
     2161 tttttaaagt ccgaatgaca gtattaatgc cgattttcag cgtacgggca gtatcgcgaa
     2221 ctccggcacc gttgaaaggc atttcagtga tttggtcttg aactcccggt ttacgggcct
     2281 cgtaacggta agtgagctga aacactcgat gacagtcacg gcagcgaaat cgatcatgtc
     2341 cttttgggtt cttcccgtga cggtaaacct gaacggactg gcaacggggg caatgaaggt
     2401 taactgtgac cattaaagaa ccttaaaagt gactattata cccctaattc aactaattgg
     2461 aggcattacc atgcattagt gcaagcgcct tgtggattct tttgctaatg gcggaaacat
     2521 attgtacaat attgcattta tccctcaata aataaatgag ttatcagatt g
//
LOCUS       DBFIFS010000355         2570 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.356, whole genome shotgun sequence.
ACCESSION   DBFIFS010000355 DBFIFS010000000
VERSION     DBFIFS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.356"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..345
                     /locus_tag="N7A07_004780"
     CDS             <1..345
                     /locus_tag="N7A07_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU2479263.1"
                     /translation="HHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELE
                     NSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQ
                     IMSRVKIVVMYN"
     gene            complement(383..1282)
                     /gene="nhaR"
                     /locus_tag="N7A07_004781"
     CDS             complement(383..1282)
                     /gene="nhaR"
                     /locus_tag="N7A07_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU2479264.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1348..2514)
                     /gene="nhaA"
                     /locus_tag="N7A07_004782"
     CDS             complement(1348..2514)
                     /gene="nhaA"
                     /locus_tag="N7A07_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU2479265.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT          749 a          675 c          564 g          582 t
ORIGIN      
        1 catcatataa gatttgactg catccagggc gagcaaggtg aattagtttt tgaacccagt
       61 gacgttgaat attactttga accagtgacg atacaggagt caggaaccag catattaaaa
      121 aatgagttag aaaacagtga ggacggtgcg ggtcaaatag gctttcaact ctccaatgat
      181 ggtacgaatg aaatccagta tggtaaaagc aattactact cttttcagca tccccatgaa
      241 ggaagtaatc agattcctct atttattcgc ccgcgtacat atggaaacaa tgtctcaagc
      301 gggcaaataa tgagccgagt caaaatcgtg gtaatgtaca actaatctat ctcccccacc
      361 ttaactttgg gggagctgcc gattaacgca ccgctggact aaaaagcgca gaataatccg
      421 tattgcagat tcgctgtacc gccgggtgct gaatcatccg ctcagcaaaa atagcatggt
      481 actcttccat cacattctcg acgcgaccaa tttctacgac agttttatcg gcataaaagt
      541 catatgcata aagcgttggg gcaacgaaga ttgcattgtg catcgcacca aaagctttca
      601 tcaaagcggc atcatcaaac tcgccaagga tttctacgtt taatccctgg gagttaaacc
      661 agttaagtaa tttgcgccct aacattgaac gtcgcccagg aatcaaaagc cgccgttctt
      721 caagacaagc tgggaacggt ttttccggcg gtggatttgt acaccagaaa ctcacgccac
      781 attcgccaat tctcacggag aacaggcctt cctgctgcgt agaatctatc ggacagtcgg
      841 aaatgatcat atccagttta tgctggctta attgctccag cagcatttcg tgggtggatt
      901 cgaagcagcg aagatgaatc ggctcgcctt ctactactgc agcattcagc acgctactga
      961 ccagacgttt ggaaagtaca tcagccacgc caacgtcaaa caataaattg gattctttgc
     1021 gatagttcac gatatccagc atttcctggc tcaaggtaaa cattttatcg gcatagcgat
     1081 agaccagctc ccccagctcg ctgggctcga gaccacgtcc cttgcgctta aataatttgc
     1141 cttgcaggcg ctcttccagc gcccgaatct gtccggtaat ggtttgtggt gttaaataaa
     1201 gcgcctccgc tgcgccaacc acagaacctt ctttataaac atgccagaag taatacaagt
     1261 ggttgtaatt gatatgagac atgctcattt ctctccctga taacaatgaa aagggagccg
     1321 tttatggctc cccggtaaac cgtcctgtca aactgatgga cgcaaacgaa cgcgtaacca
     1381 gctgtatcca attaccgccg aagagataga accgaccagg ataccgagtt tcgcccagtt
     1441 aatcagttct ggatctacgc taccaaaggc caggctggca ataaagatag acatagtaaa
     1501 accgataccg cacaggatcc ccaccgccat aatttgctga taagtcgttc cctcaggcag
     1561 atgcgccagt ttcaaacgca gcgccaacca gcagaacaga ctaatcccca gcggtttgcc
     1621 aatcagcaag ccagcgatga tccccaatgg cagaatggag gtcaagccat ccagcgtgac
     1681 gccttgcagt gaaacgccag cattagcaaa tgcaaacagt ggtaaaatca gatacgccac
     1741 ccacgggtgc aacacatgct ccagtcgctt cgctggagaa cgcccatgct tctctttcaa
     1801 aggaataaag aagccgacaa ttacccccgc cagggttgcg tgaacccccg atttcaacac
     1861 tgcagtccac aacactacgc caaccagaat atagacgccc gtgcggcgta caccacacag
     1921 attcaatacc gcgagtacag caattgctac agccgcgacg ccaagagagg ccatcgataa
     1981 gtcattagtg tagaacaatg cgatgataat gatggcccca agatcgtcga taatagccag
     2041 agccatcaaa aagatcttca gcgctaacgg aacacgactt cccaatagcg ccagcacacc
     2101 aagtgcaaag gcaatgtcag tagccgccgg gatcgcccac ccttcgcggg taatcggatc
     2161 ggcatagtta aaggccagat agagtaatgc cggcacaatc atcccaccaa tagcagcgat
     2221 aactggaaat gcggcctggc gtaagctggc tagcgatcct tgcatcagtt cacgtttaac
     2281 ttccagaccg actaacagga aaaataccgc catcagcgcg tcatttatcc ataacagcat
     2341 gtttttgttg atttcgagtg aaccaacccg gagctgaacc ggcgtctcca gaaagtcgtg
     2401 ataccatcca ctggttgcgc cgctgttggc catcatcatc gccaggatag cggcaatgat
     2461 aagaataatg cctcccgagg catcactgct aaagaatcga tgcagatgtt tcacttttta
     2521 tttctctttc aggtgaatag atcgttttta ctattttacc ccgcacgaat
//
LOCUS       DBFIFS010000356         2529 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.357, whole genome shotgun sequence.
ACCESSION   DBFIFS010000356 DBFIFS010000000
VERSION     DBFIFS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.357"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(1..1721)
                     /locus_tag="N7A07_004783"
                     /pseudo
     CDS             complement(1..1721)
                     /locus_tag="N7A07_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1667..1888)
                     /locus_tag="N7A07_004784"
     CDS             complement(1667..1888)
                     /locus_tag="N7A07_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479266.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(1933..>2529)
                     /locus_tag="N7A07_004785"
     CDS             complement(1933..>2529)
                     /locus_tag="N7A07_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU2479267.1"
                     /translation="ALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPD
                     ADAEAAFGQFVTANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLP
                     VIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSM
                     FQTGSVAIRAERWINWRRRRTAAVAVITGVNYGTASGG"
BASE COUNT          509 a          830 c          623 g          567 t
ORIGIN      
        1 tttttgatcg tcaatggcgg ggagtatttc cagtgccgtg gcatcacgcc cgtcctgcgg
       61 agccggcagg gcactcaccg catcattcac cgccttctgc agaacatccg gatcgtagtc
      121 acgaccgtcg cgcggaacag gaatatggct cactgcctct ttcaccatct gctcaagcat
      181 cggacgcaca tcatcacccg tcacgcactt ctgtaatacc acagactgag aagccagttt
      241 ttcttcaaac gtttgtgcct gcgcggctat tttctcctca aatgtacgct gtaaatccgc
      301 cagcaccatg gcgaattctt cgcccagtgc acgaataatg gacagttccc gttccgtcat
      361 tttcgcagta tccccctgaa catcgctttc accgcatcat gctctgtttc actgattgcc
      421 ttattaccgt cagatgcgcc atcaggcagt tgtgctgaga ctgttttccc ggacgacgcg
      481 aacggatcct cacgggcatc acgacgggac agcgcctcca gactgtagtt ctgctgctga
      541 agatacagtg catcaccgcc ggccaggggc ggcaggttct cacgtttacg ggcctcattg
      601 ggcgtcagca gcgtattttt caccgactca cccagcgttt tcatgcgccg ttcgctgtcc
      661 attctcagca gcgtggtgac gtcaaactcc gtgctctcgt tttcccccgt ttccagcgcc
      721 tcatccagta acagctcaat ggactcaatc agcgtctgca gacactggga ataatactgc
      781 tgctccagcg cctccacgtt atcactggag ggaggttgtc ccacaccaat cttgtaggcc
      841 gggacacgga acaccgaaca gacaatttca gcggtcattt tcagttgttc caccgtctgc
      901 gcatccaccg gtgaaaacgt cgtggggtta tatttcgccc cgttgctcag aatcgccgtt
      961 ttccccgcat tttcgccggt atacccgctg tcccagttgc tcttcagttt tttcgcattt
     1021 tcttccgtta tactgccggg gatctcaatc accccggacg gcctgccgcc atttctgaaa
     1081 aaagacgttg aatttgcctg aatatgatac ccctgcgtgg cagccagccc ggcagcatac
     1141 accggcggca gccccacaag cggatgaaaa aaacagttaa accggtcgtg gatcacttcc
     1201 cgggcaggca ccgtcaccgc ctccgtgatc ccgcagttcc ggtccggcgt gatgcgatag
     1261 aacacctcgc cgtcatccgc caccagaggt tcaacccggc tccagtccag aatacgcagt
     1321 tctttgatct gcccacggga gttacggatt ttcagcacca ccgtattgcc gtgacgcagt
     1381 ttggcgttca gccacagttc aaaaaactgg atacgattct gctgtgcatt gggacgacga
     1441 cagagacggg caatatcccc ctgccgtttt tcacggcgga tcccctgtgt atcggtctgc
     1501 atcaggcgca gccgcatttt ggcgatatcc tgggatatca gcgaaatgca agaaaacacc
     1561 gcatgaaagg agaggacact ttccggatcg gctttcactc cctgctgcca ggcaccggca
     1621 aaaggctcag ccaccgcctg aaacaggctg gtccagccca cctcttttac atcacgtcct
     1681 gatttctggt tttttcgggg tcgccgcaaa aggttccaca ttcgccatgc tccgcatcac
     1741 gtttcttttt ctgacctgcc ggacgtcgcg ctgtgatgta ctccgccttc cccaggcgaa
     1801 ccagcacctc cgcacacggc tgtgccacat cacggatatc cccggcccgg gcatcatgcg
     1861 tgccctgcag atactggatt tttgccatca gttactgcgg gaagctagcg cctcccgccc
     1921 tcctcatcag actcagccgc cggacgcagt tccgtagttc acaccggtga tcacagccac
     1981 cgccgcggta cggcgacgac gccagttgat ccagcgctcc gcacggatgg ccacgctgcc
     2041 ggtctggaac atggagacca gctccaccgg tgacggtgtg ctgctgtcgc tggtcggttc
     2101 agactgcatc tccagtgacg cttcacggga catatccacc gccacaccac cgtcatccgc
     2161 cagataaata tccggcgcat tcaccagtac cagctggtca cccacatact gggagacaat
     2221 caccggcagc ccctggaagg tcccgcccag cagggtcatg tccggatatt ccttctgccc
     2281 cagcgcattt ttacgcatgg acagcgccag ggcattcgtg ctggacatca gccagaccgc
     2341 accggtgggc tgcagatttg ccgtcacaaa ctggccaaac gcggcctcgg catccgcatc
     2401 cggattaccg cttgacgccg tgcccttcac atcatgggtg atggacgcag gggagacatc
     2461 tgccaccgcc gcttttttcg ggtccacaaa gtctgtatcc agacgcgcca ccaccgcttc
     2521 cgccagcgc
//
LOCUS       DBFIFS010000357         2524 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.358, whole genome shotgun sequence.
ACCESSION   DBFIFS010000357 DBFIFS010000000
VERSION     DBFIFS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.358"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..770)
                     /gene="chbR"
                     /locus_tag="N7A07_004786"
     CDS             complement(<1..770)
                     /gene="chbR"
                     /locus_tag="N7A07_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU2479268.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLF"
     gene            complement(778..1128)
                     /gene="chbA"
                     /locus_tag="N7A07_004787"
     CDS             complement(778..1128)
                     /gene="chbA"
                     /locus_tag="N7A07_004787"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU2479269.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1179..>2524)
                     /gene="chbC"
                     /locus_tag="N7A07_004788"
     CDS             complement(1179..>2524)
                     /gene="chbC"
                     /locus_tag="N7A07_004788"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU2479270.1"
                     /translation="ASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLIN
                     NVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALA
                     EERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTF
                     IVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTIS
                     TPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVE
                     AALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIF
                     QINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLG
                     AFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT          702 a          650 c          545 g          627 t
ORIGIN      
        1 ataaacaaac tcgggctact ataaccggcc tcaaacgcaa tatccgtcac tgaatagttg
       61 gtcatttcca gttgtttttt ggcaaaatta atacggattt cattaataat ctgcattggc
      121 gttttgccat aatatcgttg agtcgcccgc gtcaaatatt cctgtgattt ggctgacaac
      181 gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc
      241 gtacttttca gccactgcgg tacatcatca atcacctgtt cttcgcggta atgacgtaaa
      301 cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa
      361 ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc
      421 tgcgaagcta caaagcaata aggcaacaat ggcaggtaat gctgctcaaa aaagcgttta
      481 ctgatcccaa cgttcaatat gcgcgtggca ccaaactcat aaaaactttg atggtgcgaa
      541 cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc
      601 tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg atgcagtccg
      661 ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc
      721 tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta
      781 tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga
      841 cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc
      901 cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga
      961 ctgatccatc atggcttttg ctgcggcaaa atcgccctgc ttcgcctgtt tcagtgcggc
     1021 atacgccagg ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag
     1081 ctcttcagct tccgtttgcg tatcgggaat attatcgaga tccatcatac atcgttcctc
     1141 tcttttctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg
     1201 atatcttctt cgctctcttc tttatcaatc gcattttgtg ctttgttagc caccacaaca
     1261 aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcaaccag caatgcgacg
     1321 acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata
     1381 ttggtcaccg gaggaataat gcccatgtag tacgctgcca gggtgattgc cgccagaatc
     1441 ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac
     1501 agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga
     1561 taatcagcac ggcgagaggc aataaagata gccaggatca ggcctaaagt cgcaccactg
     1621 cctccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg
     1681 gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc
     1741 cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag
     1801 aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc
     1861 agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac
     1921 gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag
     1981 gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca
     2041 ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat gatattcgcc
     2101 ccacctaacc agtttgcgcc aaccgcatag gcctcaccga cactatatgg ggtgacggtc
     2161 ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc
     2221 agcgccatgc caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat
     2281 acgttgccgc caataccttt cagaccatta agtgtttcaa tggttgaggc gtcgaggcga
     2341 atacctaagg aataaaaaaa cgacccctcc ccaaagctta gaaaaacgtt gttaattaat
     2401 acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca
     2461 ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat
     2521 gcaa
//
LOCUS       DBFIFS010000358         2521 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.359, whole genome shotgun sequence.
ACCESSION   DBFIFS010000358 DBFIFS010000000
VERSION     DBFIFS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.359"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            948..2093
                     /gene="espG"
                     /locus_tag="N7A07_004789"
     CDS             948..2093
                     /gene="espG"
                     /locus_tag="N7A07_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HGU2479271.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
     gene            2221..>2521
                     /locus_tag="N7A07_004790"
     CDS             2221..>2521
                     /locus_tag="N7A07_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU2479272.1"
                     /translation="MKRKPLIAFFIAIFIALTILILFPFNCTYKKSPQEHSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPLPFISDAETIEHIQ"
BASE COUNT          851 a          523 c          434 g          713 t
ORIGIN      
        1 ctgaaccgtt tcactatact ctacttaatt cgagttacag gaaggcggca agcgagtgaa
       61 tccccaggag cttacataag taagtgattg gggtaagcga acgcagtcgc agcacatgca
      121 acttgaagta tgacgagtat atatatctat tagctgtttc ccggacataa gctacagcca
      181 gcactaacct ggaacgggtg cattataaaa aggaacaagg gacaaatagc tgaacagtaa
      241 ccgcgttagt ctcttcccgc ataaccgtac cattcgtatt aagaagtatt gcccatatcc
      301 attcggcgaa agaggatctg ccatacatca agtccgtttt acttggcatc agatcatcgc
      361 cgtcattaac tctgttaaag tcggacaaac gattagcaag atctgtgatt caaaagtgct
      421 tagcgctaag gtgggactgg ttacagattc attaaccctg ttctttgtcg cacctatcga
      481 tcggtgtaat gccacaacag gtttatgtta atcagaacaa tgcgcccctc tataaagaga
      541 attctttgat aaggcaactt gaaaagggaa agctaactga tatcaatgta ccactttagt
      601 aatcatcaca ttatatacag gccaaaataa gtttcctgaa agccagtatc tctaaatcaa
      661 tcattaatga tatattttat cgtcacaata aaaaattatc taaattctat tacttatgta
      721 aataagtaca accttttcat agacacaaaa actatggctg acgcatcact tgcaatcaat
      781 tatccatcat tagattaata ttttcactta tttttcagaa ggtaaaaata actttctatg
      841 cgtttaaaac acccgctata aatatcttgt atattattga cactatatga tgctatatga
      901 tacttgttgc caaattgttc attacaaacc agataggaga atctctcatg ataaatggac
      961 ttaataatga ctccgcatct ttagttttag atgctgcaat gaaagttaat tctgggttta
     1021 aaaaaagctg ggatgagatg tcatgcgctg aaaagttatt taaagtactt agttttggtt
     1081 tatggaatcc aacgtacagt cgtagtgaaa gacaatcatt tcaagagttg ttaaccgttt
     1141 tagagcctgt atatccactt cccaatgaat taggcagagt atctgctcgt ttttcagatg
     1201 gttcatcctt aagaatttcc gtcactaaca gcgaacttgt tgaagccgag attcgcacag
     1261 caaataatga aaagattact gtgctcctgg agtcaaacga acaaaatagg ttattacaat
     1321 ctttacccat cgatcgccac atgccataca ttcaggttca tcgtgcctta tctgagatgg
     1381 acctgactga tactacctca atgcgcaatc tacttggttt tacgtcaaaa ctatcaacaa
     1441 ccttgattcc tcataatgct caaacagatc cgctttccgg gcctacacca ttcagctcta
     1501 tctttatgga tacatgtcga ggactcggca atgcaaagct ttcactcaat ggtgttgata
     1561 tacctgcaaa tgcacaaaaa ttgcttcgcg atgcactagg acttaaagac acacattcat
     1621 caccaacccg gaatgttata gatcatggta tttctcgcca tgatgcagag caaatagcaa
     1681 gagaaagcag cggcagtgat aaacagaaag ctgaagttgt ggaattttta tgccatccag
     1741 aagcagcaac ggccatatgc tcggctttct atcaatcttt caatgtgcca gccttaacgt
     1801 tgacacatga aaggatctct aaagccagtg aatacaatgc ggaaagatca ttagatacac
     1861 ctaacgcttg cattaacatc agtatctctc aatcatcaga tggaaacatt tatgttacca
     1921 gccatactgg ggttctgata atggcgccag aagaccgccc caacgagatg ggcatgttga
     1981 cgaacaggac ttcttatgaa gtgccgcaag gtgtgaaatg tataatcgat gaaatggtaa
     2041 gtgcgctaca accaaggtat gccgcatctg aaacgtactt acaaaacact taaaataaaa
     2101 ctttattaat caaaactaat aatagaaacc acacccataa ttattaacaa tttattccga
     2161 agttaatcac aattaaacaa agatatttat tctgcaaaca atttacagca aatagagaaa
     2221 atgaaaagaa aaccattgat tgcatttttc atcgcgatat ttattgcact aaccatatta
     2281 atattatttc catttaattg tacttacaaa aaatcacctc aagaacactc actttcattt
     2341 ataaaacaat taccaaatac ggtgaactta tcatctctta cttataataa agaggatgat
     2401 ttcctgtatg ccacccagaa tagccctgca cagctactta aaataaccaa aagtggcgac
     2461 atcatggaca gagccccact gccttttatc tctgatgccg aaacaattga acacatacaa
     2521 g
//
LOCUS       DBFIFS010000359         2442 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.360, whole genome shotgun sequence.
ACCESSION   DBFIFS010000359 DBFIFS010000000
VERSION     DBFIFS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.360"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..432
                     /gene="paa"
                     /locus_tag="N7A07_004791"
     CDS             <1..432
                     /gene="paa"
                     /locus_tag="N7A07_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU2479273.1"
                     /translation="KNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIE
                     TVAAIRKNIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIW
                     RDMNITVRQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            768..923
                     /gene="hokD"
                     /locus_tag="N7A07_004792"
     CDS             768..923
                     /gene="hokD"
                     /locus_tag="N7A07_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2479274.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1144..1401
                     /locus_tag="N7A07_004793"
     CDS             1144..1401
                     /locus_tag="N7A07_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479275.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1471..1749
                     /locus_tag="N7A07_004794"
     CDS             1471..1749
                     /locus_tag="N7A07_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479276.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1751..>2442
                     /locus_tag="N7A07_004795"
     CDS             1751..>2442
                     /locus_tag="N7A07_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2479277.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          621 a          587 c          667 g          567 t
ORIGIN      
        1 aaaaatatcc ggagtattga cgacctgacc agacctggga ttggcgtaat tgttaatgat
       61 ggtggtggta ccagtaatac atcaggcact ggcgtctggg aagatattgc cggacgtaaa
      121 gggaatatag aaactgtcgc cgcaatccga aaaaatatta ttttatatgc gcccaatagc
      181 ggaactgcac gtaaggctct tgagaatcag cctggagcag atgtctggat aacctgggct
      241 gactgggcag ccagtaatcc agaaattggt gatgtcgtgg aaatagcgcc agactacgtg
      301 atatggcgtg atatgaacat tacagtacgt caggatgcaa atgatgaaac ccgtcgattt
      361 gcagaatggc tacaaaccga tgaagcggcg cctgcattca aaaaatatgg ctggaccagg
      421 aaaggcactt gacatcctcg tccttcagga cgtggattct ttttccggat gccgcgccag
      481 cggcatgtag gggcagctca caaaacggaa aaaattgtac gctaagcctc gccaggtgaa
      541 ctgaattcat tccgatatgg gaattcccat atcgggcgaa aacggtttgc tgtaacggca
      601 gagttaagta ggattgctgc gggtgcttga ggctatctgc ctcgggcatg aacaccaacg
      661 gcagatagat aaaagcccca cccgactata aatcgaagtg aggcccctat atgctcgtca
      721 catatagatt gcctcttacg gaccgaaagg tcaaggagaa gcaggctatg aagcagcaaa
      781 aggcgatgtt aatcgccctg atcgtcatct gtttaatcgt catagtgacg gcactggtaa
      841 cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca gacggaggtc gctgtcttca
      901 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
      961 tgtgtcaggc atcctcaacg cacccgcact taacccgctt cggcgggttt ttgtttttat
     1021 tttcaacgcg tttgaagttc cggacggcgc cggaatagaa tcaaaaatac ttaagtagcg
     1081 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1141 ttgatgagaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1201 gttgagaagg gcgctattgt cagccactgc cgtattaaat gtttacagtc tcaacaaagg
     1261 aagagtggag ctgcgttaat tcattttgtg gatgggcttg tgacggatgg ttttattttg
     1321 cgtgcaaatg aatttgtcac atcgttgccg tctctgaaag aagctgggat taaggctggt
     1381 ttttctgctt ttgaagatta gtgaattcat ctacaattca gcgcagggct gaacccctgt
     1441 tgagtaacac tgtgccaccg gagaaagccg atggcgcaaa attccagact acacaattct
     1501 gataattcag ccgtctttgc cagcaggcac gggcggcgtt ctcatgcatt caaatctgac
     1561 tggttccggc acgccccatg cactgaagaa caggccgaat ggctgattca gaactaccgc
     1621 agacgtgggt atgagtttag gaaagccctc agcctcgatt atcgtcactg gataatctac
     1681 gtcaggctgc cgtactccga gcgcccaccg cgtccgtccc gcacattcca gcaacgcatc
     1741 tggaggtaac gtgcgggtat tacttcgacc tgttccggta ccggaacttg gggtggtggt
     1801 ccttaaacca ggccgtgaat ccatgcaggt attccataac cctcgagtgc ttgtggagcc
     1861 ggagccgaaa agcatgcgcg gtctgccgtc cggcatcgtc cctgccgttc gccagccgct
     1921 ggcggaggat aaatcattac tgccgttttt cagcaatgag cgtgtgattc gtgctgttgg
     1981 tggcgctggt gcactgtctg actggctgtt gcgtcatgtc aaatcctgcc agtggcctca
     2041 tggtgactat catcacagtg aaaccgttat tcaccgttat ggtaccggcg caatggtgtt
     2101 gtgctggcac tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc
     2161 tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca
     2221 ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga
     2281 cgcgctaccg gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaatccgctc
     2341 aatgtaccgt gaaagcgaca tcgtaccggg agagcagacc gccaccatca tactgaagca
     2401 gcgcacaaaa aatcttgcgc cgctgcctca cgcccaccag ca
//
LOCUS       DBFIFS010000360         2435 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.361, whole genome shotgun sequence.
ACCESSION   DBFIFS010000360 DBFIFS010000000
VERSION     DBFIFS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.361"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(155..328)
                     /locus_tag="N7A07_004796"
     CDS             complement(155..328)
                     /locus_tag="N7A07_004796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479278.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            506..709
                     /locus_tag="N7A07_004797"
     CDS             506..709
                     /locus_tag="N7A07_004797"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HGU2479279.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(641..1105)
                     /locus_tag="N7A07_004798"
     CDS             complement(641..1105)
                     /locus_tag="N7A07_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479280.1"
                     /translation="MPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGTFFS
                     LVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRTVTE
                     VNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1034..1774
                     /locus_tag="N7A07_004799"
     CDS             1034..1774
                     /locus_tag="N7A07_004799"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU2479281.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2008..2415
                     /locus_tag="N7A07_004800"
                     /pseudo
     CDS             2008..2415
                     /locus_tag="N7A07_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          635 a          598 c          603 g          599 t
ORIGIN      
        1 ttttaaggct tactgactga atattcaaaa tgtgttacat gttatcaaac ctttaaagaa
       61 taacctgctc ttcttcattg gccggattta gctcaatctt gtcagcaaaa ctaatataaa
      121 gccgttcata aagagaagga tgtagcttcg ttatttatct tttacatgta acagtgccag
      181 gggatattcc gcacatctct gtggctatca tccccaccac gtctccagtg gcagcacctg
      241 cggcactggc acgggcgtta tttccctgta ccaccgccag tgcagcattc accacggcat
      301 gagctgccac atttgcagtc ttattcacca cacctgtaac ctgtctctta tacacaaatc
      361 cccacccggg gatttgtgat gtctgtcagt ctgctttagg gggactcttt ccatccggta
      421 gtttccttcc tccccggcag cgattttttg aactttttct tcacccgttg atcttctctt
      481 atatttcatt ttcttcgggt ctgttatggt actttctgat tgttattcac tggctaacga
      541 gcagtctgga catgcccgtc tcggtgatcc gcgtcgtaca cgacgccttg tgtcactgac
      601 ctcttctctc gctcagcatg caggtctttc tattgtgaaa tcatcccact tcactgctca
      661 ggttgaaggt gcgtaccgcc tgatacgtaa cccgtcggtg tccccgtaag ccattgccga
      721 agccggattt actgccactg caagggcatg tgaggcacat ccgctgctcc tcgcgctgga
      781 agataccacc accattaact tcggtcacag tacggcattt gatgacctgg ggaataccac
      841 cactaacccg aagacgcacg ggctgctggc tcactctgtc ctgatgtacg cccccgacag
      901 cgccctgcca gtcgatctta tcgaacagca gtgctggagt cgtgccacgg acacctacgg
      961 tgttaagcac cagcgaaaag aacgtccctg tgaagaaaaa gaaagttacc gctggcagca
     1021 ggcttcggag cgcatggccg aacggctgag ggagatacag aaacaggtaa tcactgtctg
     1081 tgaccgggaa gcggatatct ggcattacct gcattataaa gtcagccgcg ggaagccaca
     1141 cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgcca ggcccggatg ctcatcagtt
     1201 acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctctcg ctcacgtatg
     1261 tctgttgcgg ggagcaggag aggacggagc ctgctggtat ccgctgacat cagaaaaagt
     1321 ggcgagtgcc gccgatgcgc gacgtatcgt cagccattac gagcaacgct ggctgataga
     1381 ggaataccac aaagcgtgga aaagtggtgg tacatgtgtg gaatcgctgc gaatgcagac
     1441 ccgggataac cttgagcgca tggtggttat cgcggtgcgg gtgctgggcc tgcggcaggg
     1501 aggtatcagt gaagaaacac aaaatgacag ctgtgagaaa atactgacgc cgacagagtg
     1561 gaaactactg tgggttaagc tggaaggaaa gcagcttccg gcgcaaacac cgaccctgaa
     1621 atgggcctgt ttgaaactgg ctaaactggg gcgatggcat gacagcaaac gcacaagcag
     1681 ccccggttgg gtagttatgt gggatggctg gttcagacat caggatatgg ctgagggtta
     1741 cctggtgatg aagtctcttg atcaggagat ctgatcaaga gacaggtcaa agatggggga
     1801 caacgtctgg tgctgtataa cagcacaggc ctcacactct tgacgcccgt ggaatatatt
     1861 agagaacaaa aatttcaata tgtggtggac gcatacagag gattgacagc attcccatac
     1921 taattgttaa gattcaaatt gaagtatatc tatctgtcat tttgcaaatg atatgcggta
     1981 gctttaccta actatcatga acatttcgaa aaatcatcca caaagccccg aagaataaag
     2041 cactatcgcc cgataaagac ctgtaagctc agtggcatgg atactattga gctcagaatg
     2101 gcgagctgta ccgttatttc attaccatgc tcgacgaaca cagcaactac tctctggccg
     2161 tactatcgct taaaagtgat atcatcaggc atttcttctg ctgtgtagcc cagttgttcc
     2221 ttgttggtat caaccagatc atcactgatg atgggcaaag agttctcggg aaaattcgac
     2281 aaaacgctac aggaagccac cgtcgggttc gtatggactt atctctatac gccgaaaata
     2341 aatgccataa gtgagcattt taaccgaaca ctgagggaac aatttattga gtttaatgaa
     2401 gaattgctct ttgaatagct gaatatgttc aatct
//
LOCUS       DBFIFS010000361         2430 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.362, whole genome shotgun sequence.
ACCESSION   DBFIFS010000361 DBFIFS010000000
VERSION     DBFIFS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.362"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            71..2350
                     /gene="ydeP"
                     /locus_tag="N7A07_004801"
     CDS             71..2350
                     /gene="ydeP"
                     /locus_tag="N7A07_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU2479282.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          624 a          577 c          623 g          606 t
ORIGIN      
        1 tccctttgta tgtactgtgc atcatcgcta ttacaaatcc taataattca tttccacaca
       61 ggataagtag atgaagaaaa aaattgaatc ctaccagggt gctgccggtg gttggggtgc
      121 tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccagg atgttattgc
      181 catgtttgac atgaataagc cagagggctt tgactgtccg ggttgtgcat ggccagatcc
      241 taagcacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg cctgggaagt
      301 cacggataag caggtaaatg cctctttctt tgctcagaat acggttcaat cattacttac
      361 ctggggagat cacgagcttg aggctgcggg acgtctcact cagcctttga aatatgatgc
      421 tgtcagcgac tgttacaagc cattaagctg gcaacaagct ttcgacgaaa ttggcgcacg
      481 ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcgggcc gcacttccaa
      541 tgaagctgcc tttctttatc agctttttgc ccgtgaatac gggagcaata actttcccga
      601 ctgctccaac atgtgccatg aaccgacaag cgtgggtttg gcagcgagta tcggtgtagg
      661 taaagggacc gtgttgctgg aagactttga gaagtgcgat ttagtcattt gcattgggca
      721 taaccctggt acaaaccacc ctcgcatgct gacttcgttg cgcgctttag tgaaacgggg
      781 agcgaaaatg atcgccatca atcctctaca ggaacgtggc ctggagcgat ttaccgcacc
      841 gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggccagtg cctactataa
      901 cgtgcgcatt ggtggtgata tggcgttgct caaggggatg atgcgcctgt taattgagcg
      961 cgatgatgct gcaagcgccg caggtcggcc ctcattgctg gatgacgaat ttattcaaac
     1021 gcataccgtc ggctttgacg agctacgccg tgacgtgctc aattccgagt ggaaagatat
     1081 cgaacgtatt tctggactaa gtcagacaca aatcgccgaa ctggctgatg catatgccgc
     1141 tgccgaacga accattatct gttacggaat ggggatcact cagcacgaac atggtaccca
     1201 gaatgtacag caactggtca atctgctgtt gatgaaaggt aacattggca agcctggtgc
     1261 gggtatctgc ccactacgtg gccactctaa tgtacagggc gaccgaaccg tcggtatcac
     1321 cgagaaaccg tctgtagagt ttctggctcg cctgggtgag cgctatggct tcaccccacc
     1381 tcatgtacct ggacatgctg caattgccag catgcaagca atatgtacgg ggcaggctcg
     1441 agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg aagcgagcgc
     1501 tgtaccgtta acgcaattag atttggcggt acacgtagcc actaagctta accgctctca
     1561 tctgctgacc gcacggcata gctatattct gccggttctg ggacgtagcg agattgacat
     1621 gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga ttcatgcctc
     1681 gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgctg tggtcgcggg
     1741 aatcgcgcag gcaacactac cccagagcgt ggtagcctgg gagtatctgg tggaagatta
     1801 tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag ttcgccgact ataaccaacg
     1861 catccgtcat cccggtggtt ttcacctgat aaatgcagct gctgaaaggc gctggatgac
     1921 gccgtcaggt aaggctaatt tcattaccag caaagggctg ttagaagatc cctcttcagc
     1981 gtttaacagt aagctggtca tggcgacagt acgcagccac gatcagtaca acacgacgat
     2041 ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg tctttatgag
     2101 tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc ttattgcgct
     2161 tacgccagac ggtaagcgca gctcacgccg catggataga ttaaaagtgg tcatttaccc
     2221 tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc taacacttga
     2281 taaccacgat ccattaagtg gcattcctgg ctataaaagt attccggttg aattagaacc
     2341 atcaaattaa tgcctcttct catttcttct gctgtcatcc gcacagcaga agaattcctc
     2401 attgattatt atcgcgcaat ttgctcacat
//
LOCUS       DBFIFS010000362         2429 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.363, whole genome shotgun sequence.
ACCESSION   DBFIFS010000362 DBFIFS010000000
VERSION     DBFIFS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.363"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1517)
                     /locus_tag="N7A07_004802"
     CDS             complement(<1..1517)
                     /locus_tag="N7A07_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU2479283.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRR"
     gene            complement(1498..1911)
                     /locus_tag="N7A07_004803"
     CDS             complement(1498..1911)
                     /locus_tag="N7A07_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2479284.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(1938..2369)
                     /locus_tag="N7A07_004804"
     CDS             complement(1938..2369)
                     /locus_tag="N7A07_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2479285.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
BASE COUNT          491 a          785 c          605 g          548 t
ORIGIN      
        1 cgacgctgct ggctgatttt ctcattcgca ccgctgtgtt tttccagcgt cctgagctcg
       61 gtttcaagcg ccagcagggc agcatgcgcc tggtcttcct gacgctcacc ggctgacact
      121 ttgactcctg acgacttcgg ctttttcagc gtcgattcat aatccttttt cgccgccgcc
      181 atcagcgtgt tgtaatccgc ccgcaggatt ttcccgtctt tcagggcctt attcagttct
      241 tcctgacggg cggtatattt ctccagcggc gtcagcagac gctcatacgc cttctgcgcc
      301 tctccggtat acttcagctg tgatgcgtcc cgttcggccc ggtccctggc ggccagttca
      361 ccggcttttt ccatatccga ctgcagcgtg gccgctgcca gccccagacg ggcattttcc
      421 cggtcattcc atgcgccctg aaggttggcc cggaaagagg cggtttttcc ccggcgctgg
      481 ctccggctct ggtaccactg ccatttttta tccgcctcat caaatgcctt ctgtgcactg
      541 gcgagcatat ccgctgagga ctcaggacga ccgatatcca gaatggcatc ccacatcgat
      601 ttgaatgcct tcccggtttt atccgcccag gtctccagtg tccccatgtt ttctttcagg
      661 cgacgggtct gctcatcaaa gcctttcgtg gcgatatcgt tcgccgcctg taaggccccg
      721 gcctcatcac cggaacgctg cagctgcgca acatacgcaa tctgctctgc cgtcacgtta
      781 cggaactggc gcaccatcgc aatcagcccc gacgtagggt cggtggtcag ttttccgaaa
      841 gcctctgcaa ccttgtccac ctccacaccg gatgcagaag caaaacgcgc gacactctgg
      901 ttgatggcat caaactgttc accaccacgc acaccggcat tcaccagggc tgccagtgac
      961 tctctcgcct ggttaaacgt cagccctgct gcctgcccgg ctcttgagag cgtcagcatg
     1021 cgatcggcag tcagtccgga ctgattaccg gaaagaacca gggttttatt aaacgctgaa
     1081 agcgtggaat ctccctggta ccaggcgtac accagcgcac ctgtcgccac cgccagcgag
     1141 gtgaccccga ccatcggcag ggtgatcgca ccggcaagcc ccctgaacat ggggatcatc
     1201 ccgccgaagg agtccttcac ctgaccgccc tgttgcagca ggatcagcca gggattctga
     1261 ccaccggcaa gctgcgtggc gatatccgta aactgtgcgg gcagggttcg catggccgct
     1321 ttatactgcc cgacggaaat cccggctttt tgtgcagcca gcgcctggcg gctcaggccc
     1381 tgttcaacag cactggcggt ttttctgacg tcggtatcca gacctgaaaa atgacgcctt
     1441 acccggctca tctgctcatc gaaacggaca gcatccagac tcaggtcaat aacaagatca
     1501 ccaaccggct gggacatatc tcacacctcc cggaatcccc gctgaagcca tcattaatgc
     1561 ggcatcatcc accatgacat ccgccacatc cgcagacgat aaaatatcgc gccctccgtc
     1621 cccaccgaac cggacgcctc cggcaagtcc tgccgctttc tgcatcagca ttttgtcctc
     1681 atccggcctc tccacctgct cttcctcatg ccgggggaca agcagactga aatcagaggg
     1741 atgcatatcc ggatcgcaaa aaaacaggct gagtacagcg tacgtcagcc cggaaaaatg
     1801 catatccagc tgggtatcct gaaaataatg cgtgcggtaa aaacggtgcc agtcggcata
     1861 ttcggtggat gtcatcccgg caagcatggc gcgccagtcg ggtctcccca tctcacgcgc
     1921 cagtctgagg gcaaagttca gctcgccgtc gaagactttc ccgcagaaaa atcatcatca
     1981 gtcagcgtgt tatttttcgc cacttcagta atatcagtat ccggacgaac agccccgctc
     2041 atcccggaca ggcacaacac cacgtcttcc gcccgggcaa tggcatcggc aggccaggtg
     2101 gtgagcactt cctgctctat cttcatcacg gcctcattca ttgacggtga ctgcgttttc
     2161 tgtggatggt tatgccacag ggacatcgcc accagaaacg cgccggttct gacgagatct
     2221 tccacgctta cctgcaggtt gccgctggat tctgcctgtt ctgcacgccg tttcaggagg
     2281 gcaagatgct cgatacgctg cagcgcagac aattcggaaa gcgtgacaga cacaccgtta
     2341 tattcaaatt gttctgtttt cagaaacatg tatgacctcc gtttaccctg cagcgcccgc
     2401 ttcagtaacg gtgacttcag ccactgcgg
//
LOCUS       DBFIFS010000363         2408 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.364, whole genome shotgun sequence.
ACCESSION   DBFIFS010000363 DBFIFS010000000
VERSION     DBFIFS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.364"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(543..1442)
                     /locus_tag="N7A07_004805"
     CDS             complement(543..1442)
                     /locus_tag="N7A07_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2479286.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1541..2263
                     /locus_tag="N7A07_004806"
     CDS             1541..2263
                     /locus_tag="N7A07_004806"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2479287.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT          677 a          529 c          553 g          649 t
ORIGIN      
        1 aatcaactta cgttacaatc actacaaagc gattgcattt aaattcaaaa cagttttctg
       61 ttgggcgcat tttttgttgt actggactaa agagaaaaaa tggggtaagg aacaaatacc
      121 ttccgtgctc aaaaattagg tttttatgca cttattaatc agtcaaggtg aacagtaaag
      181 tgttcacttc ttatcaatcg ttaatttcat atctttttga atattaatga aaaaaaacac
      241 aatgagtagt atgaacagtt atcccccaaa aaaagttttt tctgccttga gaatgcgcgg
      301 aaagcgaacg ccttcaccaa tgtgtatagg ggtgtgtgta gtttaaatct tctattttaa
      361 tttatgtatt aaaatcattg tgttggtggc gattattggt tcctattatc gcaccattta
      421 aatcaagtag ttaccccata tttaaataca ccacgtttcc tcctgtgccg catttgtgcc
      481 attgtaaccc tggcaattaa tcaaaatact gttctgacat caggcaatgc aggtgcagac
      541 atttaagcca attgctgccg ccattcctta acgtggtcaa tcagagcgcg gagctttggt
      601 gcaatattgc gacgctgtgg gaaatacaga tagaagcccg gaaattgtgg aagaaagtca
      661 tcaagcagag atacaagctt accgctttca atatacggcc tgaaagtttc ctgagtggca
      721 attgttattc ctccgccggc aagagccagc ctcaacatca gacgcagatc attagtcgta
      781 atctgcggtt caatcgcaag gtcgaaagct ctcccgtttt cttcaaatgg ccagcgataa
      841 ggcgcaacct ccggggactg acgccagccg atacacttat gattcacaag ttcacgcgga
      901 tgaacaggtg tgctgttggc tgcgagatag gagggagagg caacgaccat ttcacgctgc
      961 ctgccggtaa ggggcacagc aatcatgtct ttttcaatga cctctccgag ccttacgcca
     1021 gcatcgtaac ctgcggccac gatatcgaat tcctcatccg tgacaacaat atcaagcgta
     1081 acggctggat tggctgctgc aaacgaagcg attagcggac ctgaaagaaa ttcttcagct
     1141 attgacgtca ccgcgattct gagaagtccg cgtggcacat ggtcagaaac catctcttcg
     1201 aacgctgcct caataccgga taaaggtaat gacagcgatt tatgcagccg ttctcccgct
     1261 tctgtcaaat taactgagcg cgtggtccgc attacaagca ttgtcccgaa tgcatcttca
     1321 aggcgtctta ttccctgact gaccgctgaa cgggtaacgc ccagccgtgc ggcggcttta
     1381 ctaaaattac tctcttctgc taccgcgata aaaatgggta aaagattaag atcgattttc
     1441 attgtttaat gccgctaacc actgagtaca ttgaaagcag gatactggaa ccaatcataa
     1501 agttctattc tctctccgaa cacatttgaa ggagaacgta atggataagg tcatattaat
     1561 taccggcgca tcaagtggta ttggggaagg tattgccaga gagcttggaa tgacaggtgc
     1621 aaaggtttta ctgggagcac gcagagttga gcgtatcgaa gccattgcaa ccgaaatctg
     1681 ccgcgcagga ggaattgcta aagcgcggga gttggatgtc acagaccgac agtccatggc
     1741 cgatttcgtg caagcagcgc tggatagctg ggggcgagtt gatgttctta tcaataatgc
     1801 gggcgttatg ccgctttcac cgcttgcagc aggaaaacaa gatgaatggg cgctcactat
     1861 tgacgtgaat atcaaaggtg tactgtgggg aattggcgct gtacttccgg tgatggaagc
     1921 acagggttcc ggtcagataa tcaatcttgg ttctattggt gccctttctg ttgtgcccac
     1981 aggcgcagtc tattgtgcat caaaatttgc agtacgggct atttctgatg gcttacggca
     2041 ggaaagctcg aaaatccgtg tgacctgcgt aaatcctggc gttgtcgaaa gtgaactggc
     2101 ttcaaccatt acccatgtgg aaaccatgaa ggcaatggac gcctaccgtt ctgttgcact
     2161 tcaacccgcg gatattgccc gtgctgtaag gcatattatt gaatctcccg aaagcgttga
     2221 tacaaccgaa attaccatca gacccactgc atctgcaaac tgaagaataa cttttacatt
     2281 cacgttttat taaataggtc agaaggaata tttatatatc cctgtgtttt tcatagcaag
     2341 atacattatg tattgctcac cccgaaggag atgaaggttt aattacagaa agattatttt
     2401 tcacagga
//
LOCUS       DBFIFS010000364         2360 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.365, whole genome shotgun sequence.
ACCESSION   DBFIFS010000364 DBFIFS010000000
VERSION     DBFIFS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.365"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(57..1223)
                     /gene="dacD"
                     /locus_tag="N7A07_004807"
     CDS             complement(57..1223)
                     /gene="dacD"
                     /locus_tag="N7A07_004807"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU2479288.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT          636 a          587 c          491 g          646 t
ORIGIN      
        1 gagtgttgat tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag
       61 gccttatggt ggaaataatc actcaggcga gaaaacatgc tgccttcccc gacagattcc
      121 agggtaacca gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct
      181 acccgctgat gggcgctaat tggcgcggtg agctctttac catcaagggt atatttggct
      241 ttgatatgtg gaatttcggc tttcggtagc accatccaga actcttgttc cgttcccagg
      301 gcgatatttt ctttatcgcc ataccagatg cgttccgtac cgaccttttt cccacggtgc
      361 aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc
      421 tcacgacctt ttgcactgtc agcccccatt accactgcaa tgagacgacg ctgcccatct
      481 acagccgaag caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca
      541 acattcatgg ttttatccca caataacccg ttacggtttt gctgggtgat accgttccag
      601 gtgagacttt tctcactgta catatgataa aactcgggct cgccgtggat gatagcgcga
      661 gaaagcacag ctaaatcata agccgagcta tgctgacctg gtgcatccag accatgcact
      721 gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca
      781 aactgccgtt gcccaccggc aatatagtca gccagtgcaa cacaagcgtc atttccggaa
      841 tccacaatta aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac
      901 atcagtgaag aaccgacaaa cactggatta tctttcgccc acgcatcgcg ccccacggtg
      961 acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc gatccacgac atagcccgtc
     1021 atcagctttg tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgaggatc
     1081 tgaccggtgg tgtaatccat caatacccag gagccggcat gaatctctgg aggctgaggt
     1141 gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt taaaaatgaa caaagaagca
     1201 gcaataataa gacggcgttt caacagcaaa ccctcaggag tttcaaatag ctgttctttt
     1261 tacggaaata cttatgaact ggctggaata aagtgcaaga aaatgtgact accctctcat
     1321 ttttatctga catgatctgt tgccactcgc tgtcaaattg tcgcgctaaa gctgattagc
     1381 acggtgatat ttgatactct ggcagacagc agaaacaacg gatttaacct aatgatgaat
     1441 gacggtaagc aacaatctac ctttttgttt cacgattacg cgcaacaccc ctgagaaccg
     1501 ccatgaacaa gtgtttcatg atttgtgatt ttagtttggt acgcaatttg gtacacaaca
     1561 caattttcac ttcagtgggt agtcatcaaa ctcaccataa cgagcatcgt tgatgatgta
     1621 tgttataacc acaaacatca catctggact agttccgttt tcatgagcgg gtatttgttt
     1681 acgcctccct ttctgcaaat cctaaagatg agattttggg gttttgggat atcttttatc
     1741 ctcagttctc tatccattat gcaaaccagt aatgaaccat caccgtgtga tagtgaggcg
     1801 tctgccacga taagcgcaga gaaatgatgc aatggtgggc ggactggctt gatgaaaagg
     1861 tagagtgagc gaccttaaac tatcgaatag aacaaagtct tgcaatccag tgcaaagctt
     1921 tgtgcgcctc agttttgtct aagcgttcta ctgaaaacat agtaaaatcg gtaacggctg
     1981 gaaatcattc aatactcgca ctatcgaaag ttcgccagcc agccgtggca cgttcttgca
     2041 tacgacgtgc cgcggatcct attattctag tatgaaatcc ctaaccgatt tcaaattctc
     2101 cagactgctc agattctctt ttatcccacg ctagcgctga taaatctcgt cattgcaatc
     2161 gacctgcgct atcgctgccg cagttcttcc agtttctgca tcgataactc catcttctga
     2221 gcaataaacc tcacctatgc aatattttat gtcaattatt tcaattgcat taatatcttg
     2281 aatggattac atagagttaa tgtatccagt actccctatt ctcttgcata taatacatgt
     2341 tgcaacttac atctcagcgc
//
LOCUS       DBFIFS010000365         2343 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.366, whole genome shotgun sequence.
ACCESSION   DBFIFS010000365 DBFIFS010000000
VERSION     DBFIFS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.366"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..237
                     /locus_tag="N7A07_004808"
     CDS             <1..237
                     /locus_tag="N7A07_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU2479289.1"
                     /translation="YLIAKKRNHPHADAIHVAGWVSLFTLHVIWPFLWIWATLYRPER
                     GWGMQSHDSSVMQLQQRIAGLEKQLADIKSSSAE"
     gene            243..1379
                     /locus_tag="N7A07_004809"
     CDS             243..1379
                     /locus_tag="N7A07_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2479290.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1376..>2343)
                     /locus_tag="N7A07_004810"
     CDS             complement(1376..>2343)
                     /locus_tag="N7A07_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2479291.1"
                     /translation="LQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFESK
                     VGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTG
                     IITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLV
                     ASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEV
                     RDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDL
                     VAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          582 a          554 c          602 g          605 t
ORIGIN      
        1 tacctgattg ctaaaaaacg taatcatcct catgccgacg ccattcatgt tgctggttgg
       61 gtgagtcttt ttacgctgca tgttatctgg ccatttttgt ggatttgggc cacgctttac
      121 cgcccggagc ggggatgggg aatgcaaagc catgattcat ccgttatgca actgcaacag
      181 cgcattgccg ggctggagaa acagctcgcc gacatcaaat cctcttctgc cgagtaatac
      241 ttatggattt actgattatt ttgacctatg tggcttttgc atgggcaatg tttaagatct
      301 tcaaaattcc cgtaaataaa tggaccattc ccacagcggc cctgggtgga atatttattg
      361 tcagcggtct aattctgtta atgaactata accatccgta tacctttaaa gcgcaaaaag
      421 cggttatttc tattcctgtt gtcccacagg tgacaggcgt ggtgatagaa gtgacggata
      481 agaaaaatac gctgattaaa aaaggtgagg tgctatttcg actggacccg acgcgttatc
      541 aggcgcgggt ggatcggctg atggcggata tcgttaccgc agaacataaa cagcgggcgt
      601 tgggcgcaga gttagatgag atggcggcga atactcagaa ggcaaaggcc acgcgggata
      661 aattcgctaa ggagtaccag cgttacgcac gcggcagcca ggcgaaagta aacccgtttt
      721 cagaacgcga tattgatgtg gcacggcaaa attatctggc gcaggaagcc tccgtaaagt
      781 catcggcggc ggaacaaaaa cagatccaga gccagctgga tagcctggtg ttgggtgaac
      841 attctcaaat cgccagcctg aaagcgcagc tcgcggaagc aaaatataac cttgagcaga
      901 cgatagtgcg tgcgccgagc gatggttatg tgacccaagt gctgattcgt ccgggtacct
      961 atgccgcgtc gctgccgcta cgtccggtga tggtgtttat acccgatcag aaacgacaaa
     1021 tcgtggcgca gttccgtcag aactccttgc tgcgcctggc tcccggcgat gatgcggaag
     1081 tggtatttaa cgctctgcca ggcaaagtgt tcagcggtaa gctggcagcc attagtccag
     1141 ctgttcccgg cggagcttat cagtcgaccg gcaccttaca gacgttaaac acagcgccgg
     1201 gttcagatgg cgttatcgcg accattgaac tgaatgagca cactgatttg agcgctttac
     1261 cagacggtat ttacgctcag gtggcggtct actctgatca tttcagccat gtctcggtga
     1321 tgcgcaaagt gttattacgc atgaccagct gggtgcatta cctttatctc gatcattaat
     1381 cttcgctttc cgccgcttgt tcaggccgca gttgctgcga ctgagcaagc tggtggcgaa
     1441 tttcacgcag catttcatcc agcacatact ggacccgcca gggctggtat tcccgtttac
     1501 ggaagatagc caccagatcc agccaccatt catacgggtg ctgaaagcag ggcaccagcg
     1561 tgccgtcttg cagatcgctc tgcacacttt tgtctggcgc aaacacaatc cccaggtgat
     1621 ttcgtgccag ctccagcgcc gattgcgtgt tatcgcaaac gtaatttccc ttcacgcgat
     1681 aatcccgcac ctctttactc ccggcgacgt tgaagcgcca gatattcgcg tcgtcgatca
     1741 tcatcgagtc gatcaaaata caggagtgat gttcgagttc gtcaggacgg ctaataggat
     1801 gtttctcaag atagcgctgg ctggcataag cggttacggc atattgggta ataaaactgg
     1861 caaccagcga ttcatctttt ggtggggcat agctgattaa aacatcacaa tcatcaggaa
     1921 attcgacgcc ttcataaaag gcattacgct caagattgca ggtttttagc gataaggtga
     1981 tatcaccgat atctttaatt ttatcgatta catgtttgga taaataggta attatgccgg
     2041 ttggggcgta gatggtgacc cgaccacgtt tctcatgctt ataatctgca ataaaattat
     2101 taagctgctc gtttctgtcc agcatgtcgt tgatgtacgg taatagcgcg gtgccaaaaa
     2161 gtgtgagcat cagttgtcgg gtggttcggt caaagacttt taaacccact tttgattcaa
     2221 aatcagcaag atatttgctg acgttggcct gtgcgatgcc aagtacagtc gcggcatggc
     2281 tgatattttc actggcagcg attaccgaga taatttttaa ctcccggtac ttaagttgta
     2341 att
//
LOCUS       DBFIFS010000366         2308 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.367, whole genome shotgun sequence.
ACCESSION   DBFIFS010000366 DBFIFS010000000
VERSION     DBFIFS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.367"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(147..1151)
                     /gene="argF"
                     /locus_tag="N7A07_004811"
     CDS             complement(147..1151)
                     /gene="argF"
                     /locus_tag="N7A07_004811"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU2479292.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1313..1729
                     /gene="rraB"
                     /locus_tag="N7A07_004812"
     CDS             1313..1729
                     /gene="rraB"
                     /locus_tag="N7A07_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU2479293.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1775..2278)
                     /locus_tag="N7A07_004813"
     CDS             complement(1775..2278)
                     /locus_tag="N7A07_004813"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2479294.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          603 a          572 c          545 g          588 t
ORIGIN      
        1 caacagcgtt taaatacact aaatcctccg gaaaatttac gtatagcaat agaaaaattt
       61 ggctggaaga aaaaaactat cactgcataa aataatttga cgccgggatg tttctgtatt
      121 tcccggcatc tttatagcga tatcaattat ttactgagtg tcgcgaccat caccgctttg
      181 atggtgtgca tgcggttttc cgcctgatca aaaacaatgc tggcggcaga ttcgaagacc
      241 tcatcagtca cttccattcc gccatgtagg ccaaactctt ccgccatttt cttgccaagc
      301 gtcgtttggt catcatggaa cgcgggcagg cagtggagga atttgacctc cgggttaccg
      361 gtcaactgca tcatcttgct gttcacctga taatcacgca gtaatgcaat gcgttcagcc
      421 catttttctt ttgcttcacc catcgacacc catacatcgg tatagataaa gtcagcacct
      481 tcaacgcctt tagctacatc ttccgtcagc gtaatattcc cgccattttg ttgtgccagg
      541 gcgcggcatt ccgtaaccag cgcagcttcc ggccagcatg cctgtggcgc gaccagacgc
      601 aaatccagac cggtaagcgc cgcagcttcg agcatcgaat tgcccatgtt gttacgtgcg
      661 tcacctgcat agaccagcgt catttcgttg aatggtttac cgggtaaatg ctcctgcatg
      721 gtgaggagat ccgccagcaa ctgcgtgggg tgaaactcat tggtcaggcc gttccatacc
      781 ggcacgccag cgtattccgc cagtgtttcg acaatctcct gaccatagcc gcgatactga
      841 ataccgtcat acatgcgacc cagcacgcgg gcagtgtctt taatcgactc tttatgacca
      901 atctgactgc cgcttgggcc gagataagta acgcgagcac cctggtcata tgcggcaact
      961 tcgaaagagc atcgggtacg ggtcgagtct ttttcgaaga tgagcgcgat gtttttacca
     1021 gtgagtttgg cttcttcttt gccgcttttc ttatcggctt tcagcttcgc ggctaactgc
     1081 agcaggctgt tgagttcagc tggcgtgaaa tcgagtaatt tcaggaaatg cttatgataa
     1141 aacccggaca tagatccctc ctgtggctaa cgtctcaatg aattaaaatt caatttatat
     1201 ggatgattat tcatttgcaa gtctaaagca taaatctttg tcacaaaggt ggaggcaatg
     1261 ccagtggtgt gtgacaatat gagtatcggc aggacattaa gaggaatgag ccatggcaaa
     1321 cccggaacaa ctggaagaac agcgtgaaga aacacgtttg attattgaag aattactgga
     1381 agatggcagc gatccggacg cgctgtacac catcgaacac catctttccg cagacgatct
     1441 ggaaaccctg gaaaaagcag cagttgaagc gtttaaactc ggttacgaag tgaccgatcc
     1501 agaagagctg gaagttgaag atggtgatat cgtgatttgc tgcgacatcc tcagcgagtg
     1561 cgcgttgaat gccgatctga tcgatgccca ggttgaacaa ctgatgacgc tggcggagaa
     1621 atttgacgtt gagtacgacg gttggggcac ttactttgaa gatcccaacg gcgaagacgg
     1681 cgacgatgaa gattttgtcg atgaagacga tgacggggtt cgccactaat taattgatta
     1741 aaatgcccga tagcacacac tagcgggcat ttttttagag ttcgcgtagc atccgcactt
     1801 cacaatcaac atggcccgtg cagccaagtg catagtcgat atgttgaaaa cctaaatgtt
     1861 catacaaacc aatcgcttct ttcaaaaaag cggtcgtttc cagatagcag cgtttgaagc
     1921 ccatttctct tgcctcctcc atcgccttta aggccagttt ttttgccagc cctttgccgc
     1981 gaatagccgg aagaaaatac atcttttgca gttcgcaaat gtccgattca ctacctgcta
     2041 atggcgcaat cccaccgccg ccgaccactt caccctcgta ctcaaccacc caatatgcat
     2101 gacccggctg actatacact tgatacagct cgtcaagatt cggatcggcg acggtgtagc
     2161 ctttatcagc ggtaagaccg tattcggcgg atacctgacg aatgacgcgg gcgatagcag
     2221 gattatcctg cagcgtcagg cgacgcatta caggtgattg cggcgcaatg ttattcatag
     2281 tttaacccaa taaaaataaa gtaattat
//
LOCUS       DBFIFS010000367         2307 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.368, whole genome shotgun sequence.
ACCESSION   DBFIFS010000367 DBFIFS010000000
VERSION     DBFIFS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.368"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..351)
                     /locus_tag="N7A07_004814"
     CDS             complement(<1..351)
                     /locus_tag="N7A07_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU2479295.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISI"
     gene            complement(403..2085)
                     /locus_tag="N7A07_004815"
                     /pseudo
     CDS             complement(403..2085)
                     /locus_tag="N7A07_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT          629 a          465 c          506 g          707 t
ORIGIN      
        1 gatcgagata ccaaccagaa actcatgttc tttgagaaag gcacaccatt cattattcaa
       61 taaaatgcca ttcgtttgta atgcattaaa aatacgtttt tggcctgcat agcgttgttg
      121 atagtgaata actttacgga aaaaatccag gccagccaga gtgggttcac cgccttgcca
      181 ggtaaaatag acctgattgc cagacgctgc gatatattgt ttgatgaact ctttcagagt
      241 gctgtcatcc atccattttt catgagtaaa ctgcgactct ttttcaaggt aaaaacagta
      301 atcacatttg agattacatt gaaaactgga gggcttggct gtaacgtgca tcgctatctc
      361 gttcaataag gcggcggaaa aatccgccgc atgaaggttt agttatttcg cttcgcttag
      421 tgctttcttg atattgttaa acttctcctg atttacctcg ctaagcggtg gctggctgct
      481 gtcgataaac tctcttacca cgccttgcat ctctttaacg acctgcggat tggcggcggc
      541 aaggttatct ttttgctgta gatccgtcag tttgtagaga cctaactgat tgttttctac
      601 tgtatagaca agctaataat cgttatttct caccgtataa gagaattggc ttaagtcctc
      661 agtgttgggg ttatgcgggt aatcgtctga ctgatgacgg acaaatttgt ggtagttatc
      721 ccagaatgga atattttcct catcaaacca gtgagaataa gaggttatcc aggtcagatt
      781 tttatgtggc tcgccttgtt tcttatcttg caaccagggc agcaaggaaa cgccatccag
      841 cttaaggtct tttggaatgc tgatatcggc tgcatcaaga gctgtcgggt agaaatccat
      901 tgcggaaatc agcttgtcat aattaccggg ttgaagtttt cctttccacc acataaacat
      961 tggggtgtga gtaccgccag gataggtctg actcttatag cctttttgcg ccccgttcag
     1021 cggcagagga ccatcgataa ccgcaccatt atcggaggta aagagaataa ttgtattgtc
     1081 atactgtccg tttttcttca gttgttcgag aatgcgtttt acaccctgat caacagaata
     1141 aacggaagcg tagtagttat ctgctgtttg actaccggta ttaaattgct tctgatattg
     1201 ctccggtgca ggattatcat ttggcaggtg cggagcatta taagccaggt aaagcataaa
     1261 aggctggtca agtgttttgg cacgatcaac aacgccaatt gcctcatcgg ttaactgatc
     1321 gctgatataa ccttttgcgg ggacacgttc acgattcttg aacagtgaag gggagttgta
     1381 atatgccgtt cctgcagcgt ggaatcccat aaagtaatca aagccacggt tttgcggttg
     1441 ccattcttcc gcagaaaatg tggtgaagtt gtcatgatag tcacgtgttt gtttatcttc
     1501 cggtaccggc acattactga tttttgacaa gtgccattta cctactgctg cagtgtaata
     1561 accatgattc tggaataatt caggcaagaa agtttctgtt agcggaatac catcctgagc
     1621 atcagtattg gaatagacac caaagcgggc gggcgctcga ccggtcatta ttgcggcgcg
     1681 ggaggggccg gaaacaccgt gcgccacata gccgttagta aaacgtacgc cttcatccat
     1741 taatgaaagg agcgtcggcg ttgatttttg cgcagcttca atggctttat ctatccctat
     1801 tttgtaggta tcgacaactt cacgattttc cattgttttt gggtcaaaag atcccttatc
     1861 aaaaggaagt tgtccataac caagatcatc catggtcagt acgataatat ttggctttcc
     1921 tttggtactg tattcagtcg gcgtaaagtc tgagaaagca acgtttgttt tggttgcttt
     1981 cagctttaca tcatctgccg catgagcagc aaatgcagcc ataccagatg ccagtatcaa
     2041 agatatcgag gtacttacga cacttttctt taatgcagac ttcataaatg ttcctcttct
     2101 tattgtactt aattccaagg taattttacg aagagaaata agtggatgta agtgaagtta
     2161 gtcacataaa gagatagcag atttagctaa aaaaagggaa ataacagtcc ataaagcgtt
     2221 gacattactt tttgttctac taagtaattt ctcgacgata aacaattaat ctattgatat
     2281 ttaataaatt attgtatttt actaaca
//
LOCUS       DBFIFS010000368         2299 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.369, whole genome shotgun sequence.
ACCESSION   DBFIFS010000368 DBFIFS010000000
VERSION     DBFIFS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.369"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..63)
                     /locus_tag="N7A07_004816"
     CDS             complement(<1..63)
                     /locus_tag="N7A07_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HGU2479296.1"
                     /translation="MKYNNIIFLGLCLGLTTYSAL"
     gene            complement(77..>2299)
                     /locus_tag="N7A07_004817"
     CDS             complement(77..>2299)
                     /locus_tag="N7A07_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2479297.1"
                     /translation="LVDASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAG
                     LLNYSFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDS
                     NKWQHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFA
                     PVIHGIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQ
                     TLYVPYSSVPVLQRAGYTRYALAMGEYRSGNNLQSSPRFIQGSLMHGLEGNWTPYGGM
                     QIAEDYQAFNLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGT
                     NIQVAGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDSNEQTQFIDYFNLFYSKRGQEQ
                     ISISQQLGNYGATFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQN
                     DRDHLLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNN
                     LNYSVQVGNTHGGNTSSGTSGYSTLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAH
                     ADGITFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNA
                     NSLADNVELDETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGEN
                     KNGSIVAENGQVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPEVSPGTLLNQQTAICR
                     "
BASE COUNT          632 a          528 c          490 g          649 t
ORIGIN      
        1 taaagcagaa taggtggtta accccagaca taaaccgagg aaaataatgt tattgtattt
       61 cataatctat tgttccttag cgacagattg ctgtctgctg gttcagcaag gttccaggag
      121 agacttcagg aagcttgtaa tcgacaatac agtttgagtt tttatcattg ccccatgaaa
      181 cctgtaattt ccctgactgt ggaagtccag tcagataaac ctgaccgttt tccgcgacaa
      241 tgctgccatt tttattctct ccgtgagtga caattgcacc gaatggaacg cttttattac
      301 cgtacttcaa cgtcattaat actttcccgc cgatttgtgc attaaatgtt gctctggcaa
      361 tagcaccgtg agttgggatg acagtgacca cggtttcatc cagttcaaca ttatctgcaa
      421 gggaattcgc gttaagagcg acacgatttt ctctatattc tgtcgcaaat ggtaatatgg
      481 catagccacg ccagtcggta tgaattccgg tctggttctc tattttgaca ttatcagcgc
      541 caggagcctt aaccagaacc attgtgtcgc ccagcggctg tccaaaggtg atgccatcag
      601 catgagcaat aatcccacca ctcattccgt aataaatctg gctgctgtca ccactccgac
      661 tgtaaccgac attagtattg ccgtaagctc cacgataatt aagagtactg taaccactgg
      721 tgccagacga tgtattacct ccgtgggtgt taccgacctg aacgctataa ttcaggttat
      781 tatccggcag cagagtgcca taaacccccg atagattggt catgccgcct ttcaaatcgt
      841 ttgacatact gtaactggcg tttgaattac gaaatgccga ctgactgtct gtacgcatcc
      901 aatgactgaa gggaacatta agcgtaaaag cgagtaaatg atcccgatcg ttttgccata
      961 tattattgga atagctgtaa ttcagcgaag tcgtaatatc accaaacggc acatttaatc
     1021 caaatgatat ttgctggtcg ctgcgtgacg tgttccagta actttggcga ctggcactga
     1081 aaaatgtcgc accgtaattt ccaagctgct gagagatgct tatttgttcc tgaccacgct
     1141 tactgtagaa cagattaaaa taatcaataa attgtgtctg ctcattgctg tctccggtag
     1201 gaggcttgac ggtgtaacca ctcattcgac tgtaggcact gtcggataag ttataaaaac
     1261 cttgcgtaga atagcgatat cctgcgacct ggatattggt tcctgtctgg tagaaggatt
     1321 tgctgtagac ggatttaacc gattgcccgc tgtgacgggt gccatctgca agtgtcgtat
     1381 tggcctgcgt gatatcgaaa gaaaaggcac caaaaagtcc taaatcttta ccaataccaa
     1441 ggttgaaggc ctgataatct tctgcaattt gcattccgcc ataaggtgtc cagtttcctt
     1501 ccagtccatg catcaagcta ccttgtatga acctggggga gctttgcagg ttatttccac
     1561 tacgatattc ccccatggca agcgcataac gcgtatatcc agcacgttgg agaaccggaa
     1621 cagacgaata aggaacatat aatgtctgaa tactgccgtc tgcctctttt atggttactt
     1681 gcagatcacc accattggcc gcagagttga tatcatcaat agtaaaaggg ccgggtggaa
     1741 cagtagtctg ataaacatcg tatccgttct gttttacact cacttgtgcg gtgccacggg
     1801 caataccatg aatcaccgga gcgaaaccat gttggctatc gggcaacatc gcttctgttg
     1861 aatttatttt gaggccacga aagttcacac tatcaaaaat atcgccatcg gtataactat
     1921 cacctaccgt taaccgtgag cgtaagggaa taatgtcacg ttcagccgac gtattgatat
     1981 gctgccattt attgctgtca gatgaattgc tactcccact gttataactc cacgttgagt
     2041 tatcgcgtag tcgccaacta ccaatgttga tgccactctg taaattcaaa tatgcatagt
     2101 tggattttcc tgcattatgg ttactacgat tattaataga attaccatta aaactatagt
     2161 ttagaagccc agcatttatt ccttcttccc acagatcagg agaaacatag ccgcgagcca
     2221 ttctgccaac ataaatttgc ggaacggaga gtgacagatg ttgctgacca acatcaaact
     2281 cagttgatgc gtcgaccaa
//
LOCUS       DBFIFS010000369         2230 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.370, whole genome shotgun sequence.
ACCESSION   DBFIFS010000369 DBFIFS010000000
VERSION     DBFIFS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.370"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            362..1339
                     /locus_tag="N7A07_004818"
                     /pseudo
     CDS             362..1339
                     /locus_tag="N7A07_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1859..>2230
                     /locus_tag="N7A07_004819"
     CDS             1859..>2230
                     /locus_tag="N7A07_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479298.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNR"
BASE COUNT          691 a          477 c          491 g          571 t
ORIGIN      
        1 gtgtaggggg gctaaaatta attctatttc attatatgag aacaccaata agaataataa
       61 tatttgatca caactgaaga tgctttttac aatgaagaat tattgttaaa aaacgctttg
      121 ttgaatcaac cgaactgttg ctgagttgca ggatcacatc acgtattttc ctgaaaacgc
      181 aggctgctta gtagcaaagc agaagttcaa aatcaccaac taaatagctg cgccgaatag
      241 tagatcaatg aaagggaact cagcccggat tgtgcgatct gatcaatcgc caaaaatccc
      301 cccctaaccg gactgagcga tgccgatcat agcaccaatt ccccgcgacg aatgacgcct
      361 gatgcaaaaa gcaatctata aaactcacga taaaaattat gcccgcaggc tgaccgccat
      421 gctgatgctg caccggagcg ctcgcgtcag tgacgttgcc agaacgctct gctgcgcccg
      481 ttcctccgtc aggcgctgga ttaactggtt cacgctgtcg agtgttgaag gtctgaaatc
      541 attacccgca ggacgttccc gccgctggcc gtttgagcat atctgcacat tgtttcgtga
      601 actgataaaa cactctcccg gatattttgg ctatcagcgt tcacgctgga gtacagaact
      661 gctggcaata aaaatcaacg agataaccga gtgccagtta catgccggaa ccgttcgccg
      721 ctggttgcca ttagtcgggc ttgtgtggcg cagggctgcg ccaaccctgc gaacccgtaa
      781 cccgcataaa gcagcaattc acaaagcact ggacgaatgt agtgcggagc atccggtctt
      841 ttatgaagat gaagtgtata tctatcttaa tcccaaaacc ggtgcggact ggcaacttcg
      901 cagacagcaa aaacatgtgg taacaccggg gcagaatgaa aaatactatc tggctggtgc
      961 tttacatagt ggaacgggca aagtcagcta tgtgggtggc aacagcaaaa gctcagcctt
     1021 atttatcagt ctgttgaaac atctgaaatt gacataccga cgggataaac ctatcacgct
     1081 catagtggac aactacatca tccataaaag tcgggaaaca cagcgctggc tgaaagaaaa
     1141 tccgaagttc agagtgattt atcagccagt ttactcgccg tgggtgaatc atgttgagcg
     1201 gttatagcag gcgcttcacg atacgataac gcgcaatcat cagtgccgtt caatgtggta
     1261 actgcttaaa aatgttcatt attttatgga aaccgtgagc ccattccccg gtggtaaata
     1321 tgggctgtta aaagtgtagc ggtattcggc gcagctattt aggatggtca ggtcgcagaa
     1381 gttctgtgcc actacagtgc gtgtactaaa caaaatgacg agggcacgaa tgtcataata
     1441 cgtgcgtatt acctgacagt agctaccaac tgagggggct tattccaaac ctgatttatt
     1501 caacaaagct ggacttatcc ttactctgaa ttattttcca gacatatgca tcatgtactg
     1561 gctcgcttgg ttcaggaaat acaggagcaa atctacagct attagattta ttcctatttg
     1621 tatgcaaagt cgcattgcat aatgcggagc tatgttacag atacgcctga cgtgcgtagt
     1681 aaaattataa agcaacatat atatacaagg agaaaccgag aaaaataaat gagtcgtatg
     1741 ttgggtagca tcgagataaa tatttttttt atcacattag caccttggtt aagggtgtta
     1801 ataataatat tctctcactt attaaaacat aaataatttt ttaagcggaa atgtaattat
     1861 gagactgcca gagaaagttc tttttcctcc tgtcactagt ggcctgtcag ggcaggaaaa
     1921 acaaaaaaaa ccgaagagca ttaccggatt tcaggaaaat tatcaacgca atatcaggcc
     1981 aatcaaaaca gcatcagaag cccgactacg cttctttgat aaaatggttt cgaaagaaaa
     2041 ctctctggaa gatgttgttt ctttaggtga aatgattcag aaggaaattt atgggcatga
     2101 acaaagaaca ttttcaccag ttcatcatac aggtaactgg aaatcatcat tgttacacaa
     2161 cgcgctcctt ggtctggcaa atgtttataa tggcttacgg gaaacagaat accctaacac
     2221 tttcaacaga
//
LOCUS       DBFIFS010000370         2224 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.371, whole genome shotgun sequence.
ACCESSION   DBFIFS010000370 DBFIFS010000000
VERSION     DBFIFS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.371"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..353)
                     /locus_tag="N7A07_004820"
     CDS             complement(<1..353)
                     /locus_tag="N7A07_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HGU2479299.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEF"
     gene            complement(610..1245)
                     /gene="msrQ"
                     /locus_tag="N7A07_004821"
     CDS             complement(610..1245)
                     /gene="msrQ"
                     /locus_tag="N7A07_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU2479300.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(1246..>2224)
                     /gene="msrP"
                     /locus_tag="N7A07_004822"
     CDS             complement(1246..>2224)
                     /gene="msrP"
                     /locus_tag="N7A07_004822"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU2479301.1"
                     /translation="SDVTAESVFFMKRRQVLKALGISAAALSLPHAAHADLLSWFKGN
                     DRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAANAGSLKTDP
                     WTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFPLHKLLALAE
                     PTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGK
                     ALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYGFYANVNPHV
                     DHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF"
BASE COUNT          588 a          538 c          547 g          551 t
ORIGIN      
        1 tggaattcaa caatgccgtc ctcaatgcca atcatctcga tatctgttgc atagcctttg
       61 tgataataat ctttaatttc agcaaatgtt ttagttttat ctgcatccgc tttcttctga
      121 aagacggggg caagtttgcc actttgcagt aaaggataaa cggattgcca gactccatcc
      181 cagtcactga gcgttcggtt ttgtacattg gcatcatcaa aaacaccatt agcagctttt
      241 tgttcgacct ctgttaaggg tttgccgtgt gagtgatgac catgcgaaaa ggcaggagcg
      301 ctaacaataa agacacctaa agcaacagcc agtttgtgaa gacgaatcgc caaaacagtt
      361 tcctccagag tcattgtata tgatgtgtaa tgttatattg taacatatga caaaatgcaa
      421 caactcttag gagatgagag catagttttc tcaggtgtac ttatctatct tattgaaata
      481 aaatgaatta tgttgataat ttgaggttga tgacaaacga tacataacct gatgtgatct
      541 gcgtaggccg gatagggcgc tcgcgccaca tccggcaggg ataacgcgta ctttgtcggc
      601 aatatgaagt tatacagata atttgttgtg aacctgcttg cgtaaccggt taaaaaggga
      661 acgcgacttc ttataccgta aggctaaaag cagtacagcc agcccagcgt agatgagcgg
      721 ctgcggtgaa ataatcttca ctgaccacag ataatgtatt ggggccagga tcgcgacaag
      781 atagacgaaa ttatgcaact gttgccagtg cttgccgagt tttcgctgca tcgcctgggt
      841 tgaagtaaac gctaaagcaa gcaaaataat ccagctgata attcctaacg ttaaataagg
      901 ccgggtaatt aactccttgc ctaataacgc cagattattc acgcccagct ccagcaatgc
      961 gtaactggtt aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca ggcggcgtgt
     1021 gcgtatcaat aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca aggtcgccag
     1081 caaaaatttc agtgcagtgc gaccggtaaa atgctgaata tctttcaccg gatcggcacc
     1141 cagtccaccg tgattgatcg cccagaccag ccagagaaac ggcaacaatc cggcaagatg
     1201 caggcaaact ttcagccatg tcacctgttt tgctgtcaga cgcattcaga aattctcccg
     1261 caaatccagg ccacgataca gcgatgccac ctggtcggcg taaccattaa acagtagcgt
     1321 tggctggcgc tgtacatcga ggatgccgcc tgaaccaata aatcgttcgg tagcctgtga
     1381 ccagcgtggg tgatcaacat gcggattaac gttggcgtaa aaaccgtatt cgtcaggcgc
     1441 tgccagattc caggtggttg gcggacgctc gcgggtcagc ttaatactga cgatcgattt
     1501 aatcccttta aagccatatt tccacggcac aatcagtcgc accggcgcgc cattttgtgg
     1561 cggtaacgcc ttgccataaa cacctacggt catcagtgtg agcggatgca ttgcttcgtc
     1621 gagacgcaat ccttcgacat aaggatattt cagcccgccg ccgataaagc ggtcctgctg
     1681 gcctggcatc tgttccggtg cataaattgt ttcgaaagcg acatacttcg cattgctggt
     1741 gggttcggca agcgccagca atttgtgcag cggaaaacca atccacggca ccaccatcga
     1801 ccacgcttcc acgcagcgca tacgataaat acgctcttcc agcgggaagc gacgggttaa
     1861 atcatcgtga tcgagggtca atggttttgc cacttcgccg ctgattttca gtgtccatgg
     1921 atcggttttc aggctaccag cattagcggc gggatcggct ttatccagcc cgaattcata
     1981 gaagttgtta taaccggaga ctttatctgc tggcgtcagt ggcaggttat tttgccaggc
     2041 ggcaggcttg ctgaactcca gcgcttttcc ggcgggggcg ggtgggcgat cgttcccttt
     2101 aaaccagcta agcagatcgg catgcgcagc gtgaggcaaa gaaagtgcag ctgcgctgat
     2161 gcccagtgct tttaacacct gccgacgctt cataaagaat accgactcgg ccgtaacatc
     2221 tgat
//
LOCUS       DBFIFS010000371         2205 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.372, whole genome shotgun sequence.
ACCESSION   DBFIFS010000371 DBFIFS010000000
VERSION     DBFIFS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.372"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..237
                     /locus_tag="N7A07_004823"
     CDS             <1..237
                     /locus_tag="N7A07_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2479302.1"
                     /translation="LGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(428..955)
                     /gene="sodC"
                     /locus_tag="N7A07_004824"
     CDS             complement(428..955)
                     /gene="sodC"
                     /locus_tag="N7A07_004824"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU2479303.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1089..>2205
                     /locus_tag="N7A07_004825"
     CDS             1089..>2205
                     /locus_tag="N7A07_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2479304.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDSPSDVVWPYT"
BASE COUNT          521 a          556 c          657 g          471 t
ORIGIN      
        1 ctgggcggtg tggcccagat gctggcccca aaggcaaaaa caccggatta ccgcgcaacg
       61 gataacggca gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac
      121 ccgatgccgg tgccttacgg tgaaatgctg gttggctccc ggcgaatctc ccaggacatc
      181 agtacccgtg atgaaggcgg tgacgggaag gtggtggtta tcgggcggca ggcataaaag
      241 cgaaaaaatc ccgcagtgct cacggacagg aactgcggga gcgttacgaa gattgagtgt
      301 aaggaattat tcttatgtca cgacaaaaac attaactcag agagggagga tgtgccgttc
      361 ttttcaggga gaaaggattt atcgtcctga ggaataaagg taaggggccc gccccttacc
      421 tgactgatta ttgaatgatg ccgcaggcca ttctcgcacc accaccgccc aggggctccg
      481 gatggtcatg atggttatca ccgccagcat gaagcatgag agaacgccct ttaatctctt
      541 ttaatgagtt cagtctcggg gccaggaccg ggtagttcgc ttttccgtca tgcgtcacga
      601 acagcgcagg gaggtcgccc aggtgtccat ccggagacca ggggccaaga tgtttgccgg
      661 tgtttttcgg gtcaaagtga ccgccagccg ataatgctgc gaccggtttt ccgtctttca
      721 gtgccggggc gcaatttcct ttttcgtgca catgaaaacc atgaatgcct tcagacagag
      781 agtgaagggc tggtgtgaac agcagaccgt agggggtctc ctgaatggtt atttttccaa
      841 tgctgacttc ttttccgtca gcactgacaa ggttcattgg gacttcctgt tctgctgcgt
      901 atccgcatga tgctgctgtc agcatggcaa tggcagcaat gattttacat ttcataaaac
      961 cctcattaat tccgttaaca gactgagctt gctggttaca gggtaacaaa cagcgttctg
     1021 atgatatcgc gcaatagctg tgcaatatcc tatcactgcg attaataata ccaattgaga
     1081 ggaacattat gggtaaaggt ggcggcaggg cgcacacgcc ggttgaggca aaggacaatc
     1141 ttaagtccac gcagatgatg agcgtgattg atgccattgg tgaagggccg attgaaggtc
     1201 cggtgaaggg gctgcagagt atcctggtga acaaaacccc gctgacggac acggacggta
     1261 atcctgtgat acatggtgtg acagcggtct ggcgcgccgg ggagcaggag cagacaccac
     1321 ctgaaggctt tgagtcttcc ggagctgaaa ccgcactggg cgtggaagtg acgaaggcaa
     1381 agccggtgac gcgcaccatt acatccgcga acattgaccg cctgcgggtc accttcgggg
     1441 tgcagtcact gttggagacc acctcaaagg gcgaccgtaa tccctcttct gtccgactgc
     1501 tgattcagtt gcagcgtaac ggtaactggg tgacggaaaa ggatgtcacc attaacggca
     1561 agaccacctc gcagtttctg gcgtcggtga ttctggataa tctgcctccc cgtcctttta
     1621 acatccggat ggtccgggag acggcggaca gcacctcgga ccagctgcag aataagacgc
     1681 tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcgattg
     1741 tggggctgca ggtggatgcg gagcagtttg gtggccagca gatgacggtg aactaccata
     1801 tccgaggtcg catcatccag gtgccgtcaa actatgaccc ggaaaaacgc acgtacagcg
     1861 gcatctggga cggcagcctg aaaccggcat acagcaacaa cccggcctgg tgcctgtggg
     1921 acatgctgac tcacccgcgc tacggcatgg gaaaacgcct gggggcggcg gatgtggaca
     1981 agtgggcgct gtatgccatt gcgcagtact gcgaccagat ggtccctgat ggtttcgggg
     2041 gcacagagcc gcggatgacc tttaatgcgt acctgtcaca acagcgtaag gcgtgggacg
     2101 ttctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggc cagacgctga
     2161 cgttcgttca ggacagcccg tcggatgtgg tgtggccgta cacca
//
LOCUS       DBFIFS010000372         2204 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.373, whole genome shotgun sequence.
ACCESSION   DBFIFS010000372 DBFIFS010000000
VERSION     DBFIFS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.373"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..514)
                     /locus_tag="N7A07_004826"
     CDS             complement(<1..514)
                     /locus_tag="N7A07_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479305.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLAAWMIDVIRHAMNGIQERE
                     LSLAELSWWAV"
     gene            complement(516..794)
                     /locus_tag="N7A07_004827"
     CDS             complement(516..794)
                     /locus_tag="N7A07_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479306.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(962..1117)
                     /gene="hokD"
                     /locus_tag="N7A07_004828"
     CDS             complement(962..1117)
                     /gene="hokD"
                     /locus_tag="N7A07_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2479307.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            1722..>2204
                     /locus_tag="N7A07_004829"
     CDS             1722..>2204
                     /locus_tag="N7A07_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2479308.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     Y"
BASE COUNT          588 a          513 c          514 g          589 t
ORIGIN      
        1 agactgccca ccaggataat tcagccagcg ataattcccg ttcctgtatg ccattcattg
       61 cgtggcggat gacgtcaatc atccaggcgg ccagattctg ttgagcaagt tgctcaagtg
      121 attcggatgt ctggtcgcgc agctggttgt cgcagtgcca gcacaacacc atcgcgccgg
      181 taccgtaacg atgtatgaca gtttcgctgt gatgataatc accatgaggc cactggcagg
      241 atttcacgtg acgtaataac cagtcagaca gtgcaccagc gccacccgca gcacggatca
      301 cccgctcatc gctgaaaaat ggcagtagtg atttatcttc cgccagcggc tggtgaacgg
      361 caggtacaac cccggacggc agagctcgca tgctttttgg ttccggctcc accagtattc
      421 tgccgttatg gaatgctgac attgattcac ggcctggctt aacgataacc agaccgagtt
      481 ccggtaccag aacaggtcga agtaataccc gcacattacc tccagatccg ttgctggaat
      541 gtgcgggacg gacgcggtgg ccgttcggag taagggagcc tgacggagat tatccagtga
      601 cggtagtcga ggctaagggc ttttttaacc tcgcatccgc gcctgcggta acactgaatg
      661 agccattcgg cctgttcttc agtgcatggg ggatgctggt accagtcaga tttgaatgcg
      721 tgaaaacacc gtccgcgcct gctggcaaag acggcagaat catcagaatt gtataatttg
      781 gtatcgtgcg ccatcggttg tctctgctgg cgcagcaggt gccagttgtt caggctggcg
      841 tgcgaattgt aaaccagaat gccaggaaaa aacaaaaccc gccgaagcgg gttaagtgcg
      901 ggtgcgttga ggatgcctga ttcatcagag gtggcgaggg atttctcccc cgccaggtct
      961 cttactcctc aggttcgtaa gctgtgaaga cagcgacctc cgtctggccg gttcggactc
     1021 gtacctcgca gaggtctttc ctcgttacca gtgccgtcac tatgacggtt aaacagatga
     1081 cgatcagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc ttgcctttcg
     1141 gcacgtaaga ggctaaccta gatttgccgt tcatagattg agcctcagat taatgttagg
     1201 cgtcttgcag gacgcgtaat gttaactggg gcttttctct atctgccgtt ggtgttcatg
     1261 cccgaggcag atagcctcaa gcacccgcag ctattctact taacttccca ttacctcgcc
     1321 aatatgaaat cggtcagaaa gacagacgtc ccataaggac aacaacggaa aaagagattt
     1381 tgcttgatgc tatttacctc tgcaccatga agttgcgcca aaatcccagt ggtgataaca
     1441 ggaatttggt tgttggtgat tggggaaaga agtacgttag actgtgggtt agttcattga
     1501 catgatagtt acctgccttc aatttcctaa gcaagcgatg taaacgataa tcaatatcaa
     1561 atgtattgac aggtaaaata ataacgatgt acctttgctg ataacatata tcagtcttaa
     1621 atcataatgg aggtagataa aataactaaa cacacacaac aaatttttta tcagtgacat
     1681 agcatatctt aagcatcctt taatgctcat ggggagaacg catgactaaa catatggaca
     1741 ttcattgtca aacacgcata tttaactcaa ttaaccatga caccaaatat aaaatatgta
     1801 tttttactcc aaaaaacatt aaaaaaaata atgcaactct ttatatactt gatgggaata
     1861 gtgccaacaa ttacatttct gatattctgc ctgttattga tgcactaccc aatccaccag
     1921 tgttagttac acttggttat gaatcttgga ataaccttag cattcatcgc cgtgcttacg
     1981 attatactcc tgatggtgaa aatgctattg ttgataactc taaaccagca tggatctact
     2041 ttactggtgg aggaagccag tctttccgag aattattact aactcagatc atgccatggg
     2101 ttagtactat tgctccgaac agttccagaa tcggtatatg ggggcactcg ctaggtgcta
     2161 tttttgtgct tgattgctta aaaaataatt catgctttaa ctat
//
LOCUS       DBFIFS010000373         2184 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.374, whole genome shotgun sequence.
ACCESSION   DBFIFS010000373 DBFIFS010000000
VERSION     DBFIFS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.374"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..770
                     /locus_tag="N7A07_004830"
     CDS             <1..770
                     /locus_tag="N7A07_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2479309.1"
                     /translation="KAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLE
                     FWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEEQWGIEDSESYCALMEHFLSGDHG
                     ANTFKANMEEAPEQVIALLNKFAVFPSDYISDCANHSSGKSSAKLIWAAELSWMISIS
                     STAFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDA
                     ELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(887..>2184)
                     /gene="gdhA"
                     /locus_tag="N7A07_004831"
     CDS             complement(887..>2184)
                     /gene="gdhA"
                     /locus_tag="N7A07_004831"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU2479310.1"
                     /translation="RDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERV
                     IQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNA
                     LTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGVGGREV
                     GFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMR
                     VSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKSSRDGR
                     VADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMP
                     TTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLD
                     IHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT          558 a          558 c          531 g          537 t
ORIGIN      
        1 ttaaagcaaa aaaagaacag ctgcattatt atcaggctaa aggtattgag ccgctcagta
       61 tcgagaaatt acaggcgcta caattgatcg caccttatcg attctatcat aagcaatggt
      121 ctgaaacgct ggagttctgg ccgcgaaagc ctgaacctgg caaagatacc ttccaatatc
      181 atgtgcttcc ttttgactcg atcgatatca taagtaaaag acgcgagtct ttagaggaac
      241 aatggggtat cgaagatagc gaaagttatt gtgccttaat ggagcatttt ctttctggcg
      301 accatggagc caataccttt aaagcaaaca tggaggaagc cccagagcag gttatcgcct
      361 tgttgaataa atttgctgtt tttccctcag actatatctc tgattgcgct aatcatagct
      421 ccggtaaatc ctcggcgaag ctaatatggg cggcggaatt atcatggatg atctcgatat
      481 caagcacagc ttttcaaaac gggacaattg aagaagaact ggcctggcat tatataatgc
      541 ttgcttctcg aaaggcgcac gagttgttcg aaagcgaaga agattatcaa aaaaatagtc
      601 taatgggatt tctttactgg catatctgct gctatcgcag aaagttaacg gatgctgaac
      661 ttgaagcttg ttatcgttac gacaagcagt tttgggagca ctacagtaag aaatgccgtt
      721 ggcccataag aaatgttccc tggggagcat catccgttaa atactcataa acgcctgaaa
      781 ttttgccggg ggcgctgcgc ttgcccggcc tacattagag cgtaactgca atgaattgtg
      841 cccatttgta ggcctgataa gcgtagcgcc atcaggcatt tacaacttaa atcacaccct
      901 gcgccagcat cgcatcggca accttcacaa aaccggcaat gttcgcgccc tgcacgtagt
      961 tggtttgctc accttcacca ccatgctcaa cacaggcatg gtggatatcc agcatgatgt
     1021 gatgcaaacg tgcgtcaact ttctcggctt tccagcccag gcgcgcagcg ttttgtgcca
     1081 tttccaggcc cgatgtagcg acgccaccag cattagccgc tttacccggt gcaaatagta
     1141 cgcctgcctg ctggaacagt tcagtcgctt cgatggtggt cggcatattt gccccttcgg
     1201 cgacggcttt aacgccatta gcgataagct gatgcgcggc gtcaacatcc agttcgttct
     1261 gggtagcgca aggcagggcg atatcaaccg gtacagacca cggctgttgg ccttcgagat
     1321 agaccagacc aaattctttg gcgtaatctg ccactcgacc atcgcggctg gatttgattt
     1381 cgataagacg tgccagtttc tctttcgtga atccgctttc atcaactaca gtgccgctgg
     1441 agtctgacgc agtgatcaca cgagcaccaa attccatcgc tttttcgata gcgtactggg
     1501 cgacgttgcc ggagccagaa acggaaacgc gcatgccttc aaaacccata ccgtggcgtt
     1561 ttagcattgc ttctgtgaaa taaaccagac cgtagccggt agcttccggg cgaataagac
     1621 tgccgccaaa tgaaaggccc ttaccggtga agacgcaggc ggtattgttg gagagctttt
     1681 tcatcatccc cgccataaag ccgacttcac gaccaccaac cccgatatca cctgccggaa
     1741 cgtcggtatc cgcgcccagg tggcgataca gttcagtcat cagcgcctgg cagaaacgca
     1801 tcacttcgcc ttcgcttttc cctttcgggt cgaaatcgct gccgccttta ccaccgccca
     1861 tcggcagagt agtcagggca tttttgaagg tttgttcgaa gccgaggaat ttaagaatgg
     1921 aaaggttaac tgacggatgg aagcgcatac cgcctttgta cgggccgatg gcagagctga
     1981 actgcacacg ccatgcacgg ttgacctgta cctgattgcg atcatcaacc cataccacgc
     2041 gaaactggat cacgcgctcc ggttcaacca gacgctccag taatgacatc tggcgatatt
     2101 ttggattttg ttcaagaaaa ggccagagtg tggtcattac ttcacgaacg gcttgcgcga
     2161 actcggtttg attcgggtcg cgct
//
LOCUS       DBFIFS010000374         2142 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.375, whole genome shotgun sequence.
ACCESSION   DBFIFS010000374 DBFIFS010000000
VERSION     DBFIFS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.375"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1127)
                     /locus_tag="N7A07_004832"
     CDS             complement(<1..1127)
                     /locus_tag="N7A07_004832"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU2479311.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGG"
     gene            complement(1191..>2142)
                     /locus_tag="N7A07_004833"
     CDS             complement(1191..>2142)
                     /locus_tag="N7A07_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGU2479312.1"
                     /translation="ARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNL
                     GYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRV
                     SLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVVRRKSE
                     ASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAE
                     AGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAIL
                     ITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          449 a          709 c          537 g          447 t
ORIGIN      
        1 atcccgccct gaccaaatcg cccgataatg gtctgcggac gcaggtaatc aataaagtcc
       61 tgtgcgtatt cctgatattc agacaggctg cctgcccact gcggatccgt ggtggtcccc
      121 gcgcccactg ccgatttcag gacatgatgc agacgactgt catccggata ctgacgacgg
      181 gccacttcca gggcttcaga tcggacgcct ttagccgcag ccagcgattt ggcaaagcgg
      241 gcgaagccaa tccccttatc cagtttctgc tccacacgga tcaccggcgc agaagccacc
      301 gcggccacat tcccgttacc ggcctgtttc accggctgcg ccgtggcggc cttaccggct
      361 tccagttcac gcaggcgctt caggtgcgca tccacctgac ggatttccgc tgcggtgttg
      421 tcgtaatgct cttcctcctc cacatccagc gtgcgccctt cctctgcggc tttggtcatg
      481 acctcctcaa gggaggctgc cagcgctgca cgcttgtttt caaaactttt aatctgttcg
      541 ccaatattca ttatggtctt ttccttatga aaaacggttg ttgactgtgc cgcagcgccg
      601 gcagaagatg cgattttcac caccggtttc cggttgccgg acgcggcaga aaacgggcgg
      661 tcgtaagatt taatggtccg gatggtgcat tccgcattcg cgggcacggt gacggcagac
      721 acctccatca gttcccagcg cagaaaatgc agtccgcctc cgtccagaaa ggtgtattca
      781 tggggacgga agcccacgga cagccccctg accagcccgg tcttaatggc cgcccagacc
      841 tcatccagcc gggcagccag ttgcgacggc atatccggta cgggcttcac cagtgttgcc
      901 gtgatttcca gcccttcgct gacccggcgc accgtacact gccccaccgg gcgggaatgg
      961 tcatgctgcc agagaaacgg gatcgcactg ccaaactccg cgccctccgg ctccaggatg
     1021 tcaccatccc gatccggaga aggcgttgac gcaatcccgg tgatcacccg ttcatcctca
     1081 ctgaaggatt tcaccgtcag cagggaacag gcccgtttaa gagtcacatc agcctcctga
     1141 aaataaaaaa accgccgcag cggttcatga tggttacagt gtgaacaggg ttatatgaaa
     1201 aaaaccgcat attctttctt tttcggttcc gggttaaggg acatcaggga gaccgcattg
     1261 aacagcgcca tcagcgggtc aatttttccc cgtccactgg cctgtttggt gataagaatg
     1321 gcgttacctt taggctccac ccgggcattg ccgacacacc aggccatcag gggctggtca
     1381 ccatgcacca gcaccccttc agccagtttg cgctcggtgg ttttaatggc cccgcccagt
     1441 ttccagccct ggcttatccc caccacaatt ccgtcgggga tcccggcttc cgccagtgaa
     1501 tccagaatct gccccacccc tgacgggtca ataccgatat ggtccagtaa ctcagcctca
     1561 tgaatgcgac gcacatattc cgccacttcc gccgtgtcat ccccgacacg ccggacaatg
     1621 gtcatatctc cacaggcaac aagatcctga aaccgggacg cctcgctctt ccgtcggacc
     1681 accgcggttt catgcgccca ggcatggccc cagcccagcc attcgcgggt ttccctgtca
     1741 cggccaatca cgtacattcc cagcagatca tccaggcccc cgccgtcaat ccccaccgtc
     1801 accacatcag cgcgctgcag gatatcgtcc aggctgacgc gcctgccctg ctgctcccag
     1861 aaatccgcac ccgcccagcg gtcagaacgc agggcaagac caatttccac attggcatgt
     1921 tttgacatga agccacgaaa tgcttcctca ccagcctccc gggctttacg gtactcccgg
     1981 tacagaaaag cctcatccac cgaataaccg agattcgggt taaccatggc gaggttttcc
     2041 atcagcaggt gagccccgct ttccaccatt tcaggaggat gctcaaaaat caccggcaga
     2101 aagtgcggat catgaatttt gccgtcacgg acatcccggg cg
//
LOCUS       DBFIFS010000375         2090 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.376, whole genome shotgun sequence.
ACCESSION   DBFIFS010000375 DBFIFS010000000
VERSION     DBFIFS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.376"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            205..1836
                     /gene="oppA"
                     /locus_tag="N7A07_004834"
     CDS             205..1836
                     /gene="oppA"
                     /locus_tag="N7A07_004834"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU2479313.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1922..>2090
                     /gene="oppB"
                     /locus_tag="N7A07_004835"
     CDS             1922..>2090
                     /gene="oppB"
                     /locus_tag="N7A07_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter permease"
                     /protein_id="HGU2479314.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLN"
BASE COUNT          604 a          492 c          501 g          493 t
ORIGIN      
        1 ataatgtgtc tcgacagggg agacacagta cgaatcgaca taaggtgatc gtctgaatca
       61 ccagaataaa taaagtcggt gatagcaatc cgtaacgata aagtaacctg acagcagaaa
      121 gtctccgagc ctgtgcaggg tcccaatccg ggattacaca tgctggttaa taccagtaat
      181 tataatgagg gagtccaaaa aacaatgacc aacatcacca agagaagttt agtagcagct
      241 ggcgttctgg ctgcgctaat ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc
      301 gtcacactgg cggaaaaaca aacgctggta cgtaacaatg gttcagaagt tcagtcatta
      361 gatccgcaca aaattgaagg tgttccggag tctaatatca gccgagacct gtttgaaggc
      421 ttactggtca gcgatcttga cggccatccg gcacctggcg tcgctgaatc ctgggataat
      481 aaagacgcga aagtctggac cttccatttg cgtaaagatg cgaaatggtc tgatggcacg
      541 ccagtcacag cacaagactt tgtgtatagc tggcaacgtt ctgttgatcc gaacaccgct
      601 tctccgtatg ccagttatct gcaatatggg catatcgccg gtattgatga aattcttgaa
      661 gggaaaaaac cgattaccga tctcggcgtg aaagctattg atgatcacac attagaagtc
      721 accttaagtg aacccgttcc gtacttctat aaattacttg ttcacccatc aacttcaccg
      781 gtgccaaaag ccgctatcga gaaattcggc gaaaaatgga cccagcctgg taatatcgtc
      841 accaacggtg cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc
      901 agcccaacct actggaacaa cgcgaaaacc gttattaacc aggtaaccta tttgcccatt
      961 gcttctgaag ttaccgatgt caaccgctac cgtagtggtg aaatcgacat gacttataac
     1021 aacatgccga tcgaattgtt ccagaagctg aaaaaagaga tcccggacga agttcacgtt
     1081 gatccatacc tgtgcactta ttattacgaa attaacaacc agaaaccgcc attcaacgat
     1141 gtgcgtgtgc gtaccgcact gaaactgggt atggaccgcg atatcattgt taataaagtg
     1201 aaagcgcagg gcgacatgcc cgcctatggt tacactccac cgtatactga tggcgcaaaa
     1261 ttgactcagc cggaatggtt tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa
     1321 ctgctggctg aagcgggtta taccgcagac aaaccgttga ccatcaacct gttgtataac
     1381 acctccgatc tgcataaaaa gctggcgatt gctgcctctt cattgtggaa gaaaaacatt
     1441 ggtgtaaacg tcaaactggt taaccaggag tggaaaacgt tcctcgacac ccgtcaccag
     1501 ggtacttttg atgtggcccg tgcaggctgg tgtgctgact acaacgaacc aacttccttc
     1561 ctgaacacca tgctgtcgaa cagctcgatg aataccgcgc attataagag cccggccttt
     1621 gacagcatta tggcggaaac gctgaaagtg actgacgagg cgcagcgcac agctctgtac
     1681 actaaagcag aacaacagct ggataaggat tcggccattg ttcctgttta ttactacgtg
     1741 aatgcgcgtc tggtgaaacc gtgggttggt ggttataccg gcaaagatcc gctggataat
     1801 acctacaccc ggaatatgta cattgtgaag cactgatggc aatacgtggg gcaggagtgt
     1861 cctgctccac ggtgtctgat ttttatcgca ttacagaagg cacaggccag aaggtagggc
     1921 aatgttaaaa tttattctac gtcgctgtct ggaagcgatt ccgacgctat ttattcttat
     1981 tactatttcg ttctttatga tgcgcctcgc gccgggaagc ccttttaccg gcgaacgtac
     2041 tttaccgccg gaagtaatgg ccaatatcga agcgaaatat catcttaatg
//
LOCUS       DBFIFS010000376         2081 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.377, whole genome shotgun sequence.
ACCESSION   DBFIFS010000376 DBFIFS010000000
VERSION     DBFIFS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.377"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..353)
                     /locus_tag="N7A07_004836"
     CDS             complement(<1..353)
                     /locus_tag="N7A07_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479315.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTA"
     gene            complement(481..1194)
                     /locus_tag="N7A07_004837"
     CDS             complement(481..1194)
                     /locus_tag="N7A07_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2479316.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1295..1495
                     /locus_tag="N7A07_004838"
     CDS             1295..1495
                     /locus_tag="N7A07_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2479317.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            1614..1907
                     /locus_tag="N7A07_004839"
     CDS             1614..1907
                     /locus_tag="N7A07_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU2479318.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            1940..>2081
                     /locus_tag="N7A07_004840"
     CDS             1940..>2081
                     /locus_tag="N7A07_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2479319.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTK"
BASE COUNT          586 a          469 c          462 g          564 t
ORIGIN      
        1 atggctgttt ttcgagtttt tcttaactct tcatgcagct ccgttctcag aatatatgat
       61 ttttcgagtt tgaaattctt gcgaccaggc gtcttattgc caggctcagg aagtggaagg
      121 cgtgtttgaa tgtcgagtgg tggacagtca tagaagtaaa ttctataaag ctcaagcggt
      181 tccctacgtt cttgtgattg acgttttcca ttaagatggg aaagaaccat tgaccatatt
      241 actttcatta tgcattgcgc agtcagttca tgctcggcga agtgtttacg atgcgtagca
      301 tgaacacgct gcatgaaaaa acccgcatca attaaaattg ctgctttctt cataagagtc
      361 ccaaaaaaat agcccagagc cgttatgcag atattaacaa ttgtctgcga acggggctgg
      421 gcttggttta attaatctat gccaagttga ctgtgccgtc aacataaaac ttacttccta
      481 tcacccaaac gtctcttcag gccactgact agcgataact ttccccacaa cggaacaact
      541 ctcattgcat gggatcattg ggtattgtgg gtttagtggc tgtagaaaca cctgaccgct
      601 atccctgatc agtttcttga aggtaaactc atcaccacca agtctggcta tacagaaatc
      661 gcctggctca acagcctgct cagggtcaac cagaattaac atcccgtcag gaaagctggg
      721 cttggatcct gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagagcc
      781 actggctttt ttggttgtgc ttacccattt ctccgcatcg cctttggtaa aggttctaag
      841 ctcaggcgag aacatcccgg cctgaacatg agaaaaaaca gggtactcat actcacttct
      901 aagtgacggc tgcatactaa ccgcttcata catctcgtag atttctctgg cgattgaagg
      961 gctaaattct tcaacgctaa cgttgagaat ttttgcaagc aatgcggcgt tataagcatt
     1021 taatgcattg atgccattaa ataaagcacc aacgcctgac tgccccatcc ccatcttgtc
     1081 tgcgacagat tcctgggata agccaagttc atttttcttt ttttcataaa tagctttaag
     1141 gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc ttttttgcgc tcatacgtta
     1201 aatctatcac cgcaagggat aaatatctaa caccgctcgt gttgactgct ttacctctgg
     1261 cggtgataat ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga
     1321 ttatgcaatg cgctttgggc aaaccaagac agctaaagat ctcggcgtat atcaaagcgc
     1381 gatcaacaag gccattcatg caggccgaaa gattttttta actataaacg ctgatggaag
     1441 cgtttatgcg gaagaggtaa agcccttccc gagtaacaaa aaaacaacag cataaataac
     1501 cccgctctta cacattccag ccctgaaaaa gggcatccaa ttaaaccaca cctatggtgt
     1561 atgcatttat ttgcatacat tcaatcaatt gttatctaag gaaatactta catatggttc
     1621 gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa
     1681 tgcttggaac tgagaagaca gcggaagctg tgggagttga taagtcgcag atcagcaggt
     1741 ggaagagaga ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg
     1801 ttgacgacga catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac
     1861 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1921 tctatcaaca ggagtcatta tgacaaatac agcaaaaata ctcaacttcg gcagaggtaa
     1981 ctttgccgga caggagcgta atgtggcaga tctcgatgat ggttacgcca gactatcaaa
     2041 tatgctgctt gaggcttatt cgggcgcaga tctgaccaag c
//
LOCUS       DBFIFS010000377         2079 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.378, whole genome shotgun sequence.
ACCESSION   DBFIFS010000377 DBFIFS010000000
VERSION     DBFIFS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.378"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            7..570
                     /gene="fimA"
                     /locus_tag="N7A07_004841"
     CDS             7..570
                     /gene="fimA"
                     /locus_tag="N7A07_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2479320.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            931..1641
                     /locus_tag="N7A07_004842"
     CDS             931..1641
                     /locus_tag="N7A07_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2479321.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1683..>2079
                     /locus_tag="N7A07_004843"
     CDS             1683..>2079
                     /locus_tag="N7A07_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HGU2479322.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVP"
BASE COUNT          586 a          460 c          444 g          589 t
ORIGIN      
        1 agaattatga aactcaaaca tgttggtatg attgtcgttt ctgtgttggc gatgtcgtct
       61 gctgcggtaa gcgcagccga gggtgatgaa tcagtaacga ccactgttaa tggcggtgtt
      121 attcatttta aaggtgaagt ggtaaatgcc gcttgtgcga ttgattccga atcaatgaac
      181 caaacggttg agctgggtca ggttcgttct tctcgcctgg ctaaagcggg tgacctcagc
      241 tccgccgttg gcttcaatat caagctgaat gattgtgata ccaatgtttc cagtaatgca
      301 gctgttgcat tcctgggtac tactgtcacc agtaatgacg atacgttagc gctgcagagt
      361 tcagcggcag gctctgccca aaatgtcggt attcaaattt tggaccgtac gggtgaggta
      421 ttaatacttg atggggccac ttttagtgct aaaaccgact tgattgatgg cacgaatata
      481 ctaccattcc aggctcgtta tattgctctc gggcagtccg tagctggtac tgcaaacgca
      541 gatgcgacct tcaaagttca atatctataa ttcaatatta aggtggctat acggcatctg
      601 aaattaaaac agggtgtgtg tggcatggct acgcacaccc gagcaattca ccaatgtgaa
      661 tctcattatt aatgatgagt ggtttgtatt tggagcgcta tgaaaaatca cataatatct
      721 aaacgttcaa taaacaacag tagttaaact tactgttatt aaaaatattt actttttagc
      781 aaggttttcc tattaaaaga gaaccaacct ttattacaaa cgcattaagt ctggaaaccc
      841 ggtgactcgc caatgtatta gcaacattta ccgattgtct gatgtaacgg ttttcccgct
      901 tatcctctta acacatggag aatgcatcag atgcaaacta cgcgtacccc ctactctatt
      961 tcctttatgg cgacagtgtt acttttattg ctctttgctt gccatagcac cgttgccaac
     1021 gcagctgtcg cactgggtgc gacgcgggta atttatccgg caaaccagaa acaggtttta
     1081 ttgcctgtca cgaataatga tcctgccagt gtctatctca ttcagtcatg gatagaaaat
     1141 gctggtgacc aaaaagacac ccagtttgtc attaccccac ctcttttttc catgcaaggt
     1201 aaaaaagaga atacgctgcg gataatcaac gccactaatc atcagttacc cggcgaccgt
     1261 gaaagcctgt tctgggttaa tgttaaagct attcctgcta tggaaaaaga ccagaagaat
     1321 gagaatacat tacagctggc aattattagc cgcataaaaa tgttttatcg gcccacgaac
     1381 ctggcaatgg cacctgaaga agcgcctgca atgcttcggt ttcgtcgcag cggtagcaat
     1441 ctcactctta ttaatccaac gccctacttc attaccgtaa caaatatgaa agcaggaaat
     1501 agcaatctcc caaacaccat ggtgccgcca aaaggtgaag tgtcggtgga tatcccgcat
     1561 gctgccaccg gggatatcag ttttcagacg atcaatgatt acggcgcatt aactccgcgt
     1621 attaaggcaa ccatgcagta agccagtgac ctaattaata ataaactcaa tcggaattaa
     1681 taatgacagc ctttcgtgcc gcatttaagg catatcgtat gcatcaggtg ttactactgc
     1741 ctcgctttgc acggcttacc attgccctta gcttagcaac tgcggtattt cccgttgacg
     1801 cagagtatta ttttaatcca cggtttttaa gcaatgatct tgctgaatct gtcgatttat
     1861 ccgcctttac taagggacgc gaggcaccac ctggtacata tcgggtcgac atttatttga
     1921 acgacgaatt tatgactagt cgggatatta cttttattac agatgataat aacgcagatc
     1981 tcatcccatg cctgagtaca gatcttcttg tcagtcttgg cataaaaaaa tctgcgttat
     2041 tagacaataa ggaacattca gcagaaaaac atgtgccgg
//
LOCUS       DBFIFS010000378         2074 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.379, whole genome shotgun sequence.
ACCESSION   DBFIFS010000378 DBFIFS010000000
VERSION     DBFIFS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.379"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..330
                     /gene="eptO"
                     /locus_tag="N7A07_004844"
                     /gene_synonym="gspS"
     CDS             <1..330
                     /gene="eptO"
                     /locus_tag="N7A07_004844"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HGU2479323.1"
                     /translation="HQSPSIHKQATVPPSEQLEQMASIVSATRYLKMRCNRSDLPDEQ
                     SILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRLTRDSTPEYIKCREFNRRLVPFIGE
                     LLARGRG"
     gene            509..1680
                     /locus_tag="N7A07_004845"
     CDS             join(509..758,758..1680)
                     /locus_tag="N7A07_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2479324.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
BASE COUNT          605 a          454 c          530 g          485 t
ORIGIN      
        1 catcagtcac cctccatcca taagcaggcc actgttcctc cgagcgaaca actggaacaa
       61 atggcgtcga ttgtctctgc gacacggtat ctgaaaatga gatgtaaccg tagtgattta
      121 ccagatgaac agtcgatcct aaatgtcgct aaccggatcg ctatcgggaa agggtggcag
      181 tcattaactc aggaggatat acgtaaacac agcgatgata tctatgtgcg tctgactagg
      241 gacagtacgc ctgagtatat aaaatgcaga gaattcaatc ggcggctggt cccatttatc
      301 ggtgaattat tggcaagggg tcgtggataa tatgaaccgc cccggaaatc ctggagacta
      361 aactccctga gaaagaggta aacagcatga ataaaaatgg gtcttccctt gtagtggtgg
      421 ctgaaggcat gatgattgta gtggcacact gaatttggcc acctgaacag aggtgatatg
      481 ctcacctcag aacaacacag gtgctccaat gaaaaaaaga aatttcagcg cagagtttaa
      541 acgcgaatcc gctcaactgg ttgttgacca gaactacacg gtggcagatg ccgccaaagc
      601 tatggatatc ggcctttcca caatgacaag atgggtcaaa caactgcgtg atgagcgtca
      661 gggcaaaaca ccaaaagcct ctccgataac accagaacaa atcgaaatac gtgagctgag
      721 gaaaaagcta caacgcattg aaatggagaa tgaaatatta aaaaaggcta caaccgcgct
      781 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      841 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      901 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagttgcat
      961 aacatcagcc atggttctgc cggggcaaga agcatcgcca caatggcaac cctgagaggc
     1021 ttcagaatgg ggcgctggct tgccggcagg ctcatgaaag aactgggact ggtcagttgc
     1081 cagcagcctg cgcaccgtta taaacgaggt ggtcgtgaac atgtcactat cccgaatcac
     1141 cttgggcggc agttcgcagt gacagagcca aatcaggtat ggtgcggcga cgtgacgtac
     1201 atctggacgg ggaaacgttg ggcatacctt gccgttgttc tcgacctgtt tgcaaggaaa
     1261 ccggtaggtt gggcaatgtc gttctctccg gacagcagac tgaccatcaa agcgctgaaa
     1321 atggcctggg aaatccgcag taaaccagcc ggggtaatgt tccacagcga tcagggcagc
     1381 cactatacaa gcaggcagtt ccggcagtta ctgtggcgtt accagatcaa acagagtctg
     1441 agtcgacgag gaaattgctg ggataacagc ccgatggagc gcttcttcag gagtctgaaa
     1501 aacgagtgga taccggtgac gggttacatg aacttcagcg atgctgccca tgaaataacg
     1561 gactatatcg ttgggtatta caacgcgctc aggccgcacg aatataacgg tgggttgcca
     1621 ccaaatgaat cggaaaaccg atactggaaa aactctaaag cggtggccag tttttgttga
     1681 ccactacatc ccgtcagccg atttacgggc cgtaagtaac gaagttggat gcaaatgtca
     1741 gatcgtgtgc gcctgttatg gctcggctgg taagagagcc ttataggcgc atttgaaaaa
     1801 actccagcta taccggaaga gatttattat taacaggaga ggtttgcttt tctgtgtagg
     1861 tttagatttt gtgtttgcag ggtaaacaaa actgattaac tcgtcatttc ggtaactgaa
     1921 atagtttgct gtattctgtt tacaatctct ttaataaggc atttgtcacg tggctattca
     1981 tatgaaaatc atacgttatc ttacaaatca atcatctgag tgttataata taacttagct
     2041 gtgatatgtg taagaatgtt taggcaatat atta
//
LOCUS       DBFIFS010000379         2046 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.380, whole genome shotgun sequence.
ACCESSION   DBFIFS010000379 DBFIFS010000000
VERSION     DBFIFS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.380"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1144)
                     /locus_tag="N7A07_004846"
     CDS             complement(<1..1144)
                     /locus_tag="N7A07_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2479325.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAA"
     gene            1413..1529
                     /locus_tag="N7A07_004847"
                     /pseudo
     CDS             1413..1529
                     /locus_tag="N7A07_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1634..1710)
                     /locus_tag="N7A07_004848"
     tRNA            complement(1634..1710)
                     /locus_tag="N7A07_004848"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1674..1676),aa:Arg,seq:tct)
     gene            complement(1724..1800)
                     /locus_tag="N7A07_004849"
     tRNA            complement(1724..1800)
                     /locus_tag="N7A07_004849"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1764..1766),aa:Arg,seq:tcg)
     gene            complement(1808..1883)
                     /locus_tag="N7A07_004850"
     tRNA            complement(1808..1883)
                     /locus_tag="N7A07_004850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1912..>2046)
                     /locus_tag="N7A07_004851"
     CDS             complement(1912..>2046)
                     /locus_tag="N7A07_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2479326.1"
                     /translation="SRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY
                     "
BASE COUNT          481 a          565 c          525 g          475 t
ORIGIN      
        1 cggctgcgtt cagaatagcg gttgccagac gatccggaat aatgctcctg cgcgcccatg
       61 aactgaaatg tgtcgggcgg tttgatgata cctggtttcc attcgttctc gatgccgcac
      121 cgtaatatcc tgatgccgga atatccggat cttctgccgg cgcgttagtg gcggtattga
      181 cgccgttacc gtctgtcatg aagggcacaa aataaacgcc ctcactctcc ctgtttttat
      241 acccgccgta cacggtgtcg tactgggtag cgtatgtatt tttccagtaa tacgtcgtat
      301 caccacaaat ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg
      361 agaggtcagc acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtgcgtggg
      421 tggcggcgct catgtcaaac tctccctgca tccagcacac cgccagcaac acatttttcg
      481 ggttcttctg taatgcagct ttagtgcgcg caatcaggtc ctgatataac ggtttaccca
      541 caccccagcg tgccgaatcc tggctggccc ccgtgtccgc actgaatgtc ccctcagcgc
      601 cctgggtgaa tgcagaacca ccacgacagc atggtaccag caggatcccc gcgttattcg
      661 ggatatacgg aagcagtttt ttggcaatat gtaagccctg gccgacacag ccgtactgcc
      721 ctttgctcag gtctgccttc ggatgattca gcgtactcat atcctgcaca tcatgcaggc
      781 agtggtcggc cggaatgatg tcgttatacg tacaactctc tccacccgga gttaccgtgc
      841 tgcggcgcgc cagctgttta atgcgcggat ccggagcatc gtaagaatcc ggtaacggaa
      901 gcccttcacc gtaggccatg ccgttggact gtccggcaag cacaaccacg tagaaccagt
      961 ccggcttaga tgaagggccg acctgtggat ctccttcaat agccaccgcc tgcatcagtg
     1021 tgtacggcgt aatggcaacc ggtccgccgt atggctgcca gccctctttc agtttgtgtg
     1081 tcagcttttc cgcaaggtct gacggcgacg ccgccctgac aacatcatag tgtttaaatg
     1141 ccatgaatcc tcccggccgg gataatattg tgagtaaaat gaggagcggg ctgaagtccg
     1201 gaagttacag gacaatggca gaagagagac aacagcccgc aatacgaaaa aggccgcgct
     1261 attgcgcaga gtgattactg tcggatatta ttcgccagct gaaatattac ttcacgtttt
     1321 gttgtttatt ccttgccgcc cgcgtctccc tgcgcgggct ttttttgtcc ataagaaagc
     1381 ccctccggag aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc
     1441 cggtgaattc agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg
     1501 cccctccgca cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag
     1561 acaacagtca accgcctgaa ttgtgagaca tttaaaaaaa aggcccgcaa aagcgagcca
     1621 gggaaaataa gtgtggcgcg ttgtactgga ttcgaaccag tgaccgattg cttagaaggc
     1681 aattgctctg tccggctgag ctaacaacgc aggatacaga taatggaccg ccttcgggga
     1741 cccgaactcc gcgcaaccag cttcgaaagc tggcgctctt tcctgatgag ctaatggcgg
     1801 tatgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gctgggtcaa aaaataataa tcagatgaaa atcaataatc
     1921 aagcccttgc ctggatacat atctgtctgg cgggaagcca taatagcggt gaaatacaga
     1981 aataaagtag gaccagcttg aataaccgca tttttctgct acagcctgtc catatccatg
     2041 ccggga
//
LOCUS       DBFIFS010000380         2040 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.381, whole genome shotgun sequence.
ACCESSION   DBFIFS010000380 DBFIFS010000000
VERSION     DBFIFS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.381"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..601
                     /locus_tag="N7A07_004852"
     CDS             <1..601
                     /locus_tag="N7A07_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2479327.1"
                     /translation="DLTLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWV
                     FMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTET
                     HDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNA
                     EMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            559..1236
                     /gene="bglJ"
                     /locus_tag="N7A07_004853"
     CDS             559..1236
                     /gene="bglJ"
                     /locus_tag="N7A07_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU2479328.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1274..>2040)
                     /gene="fhuF"
                     /locus_tag="N7A07_004854"
     CDS             complement(1274..>2040)
                     /gene="fhuF"
                     /locus_tag="N7A07_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU2479329.1"
                     /translation="YEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFIRLDEPAPL
                     NAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGLMVPPLMLA
                     LLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETLISQALVPV
                     VQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTTGEDN
                     PLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
BASE COUNT          513 a          476 c          511 g          540 t
ORIGIN      
        1 ggaccttacc ttattacaat tacgtcgttc cggattagtc attgctgatt tagccggtga
       61 aagtgaagat ccacgttctg tttgtgaaca ttattattct ttaatctcac aatatcggga
      121 aattcactgg gttttcatgg tgtcacgctc ttggtattcc caggctgtag aactgctcat
      181 gtgccctacg gcgacgttat tgtctgatgt tgaacccatt gagaatctgg tcaagaccgt
      241 acgttccggc aatacgcacg cagagcgtat cagcgccatg ctgacctctc cggcaatgac
      301 tgaaactcat gattttagct atcgctccgt cattctcact ctttcagagc gcaaggtact
      361 gcggctatta ggtaaaggat ggggcatcaa ccagatagct tcattgctta agaaaagtaa
      421 taaaactatc agcgcccaaa aaaacagtgc gatgcgtcga ctggcaattc acagcaacgc
      481 tgaaatgtat gcatggataa atagcgcgca gggtgcaaga gaacttaact tgccttctgt
      541 ttatggagat gccgcagaat ggaacacagc cgaattaaga agagaaatgt cgcactcata
      601 gaaaaatgcg tcatgagtag tatcggtatt gagagtttat tcagaaagtt tgcgggcaac
      661 ccttataagc tccataccta taccagtcag gagtcatttc aggatgccat gtcgcggatc
      721 tcgtttgcgg cggtcatttt ttctttttct gccatgagaa gtgagcgcag agagggatta
      781 tcttgcctga ctgaactggc gattaagttt ccgcgtaccc ggcgtttagt tattgcggat
      841 gatgatattg aagctcggct gattggttca ttgtcgccat caccgctgga cggtgtatta
      901 agtaaagcgt caacgctgga gatttttcat caggaactct ttttgtcatt aaatggtgta
      961 cgtcaggcga ccgaccgact gaacaatcag tggtacatta accaaagccg gacgttaagc
     1021 ccgacggaga gagaaatatt gcgctttatg tcgcgtggct actcaatgac acaaattgcc
     1081 gagcagctta aacgcaatat caaaacgatc cgtgcacata aatttaatgt gatgtcgaaa
     1141 ctgggcgtca gttctgacgc agggttgttg gaggccgcag atattctgtt atgtatgcgg
     1201 cattgcgaaa caagtaatgt gttgcatccc tattaatccg catgatgccg ggtttacttc
     1261 cccggcagtg ctttcatttc agcgtacaat cgccacattg ctgtacatcc ggtaagcgat
     1321 aacgctggca gcaagtgcgg cgcaccagca ggccgtcgcg tagcaccacg gtacgccaga
     1381 gtggattatc ttcaccggta gtaagcgttt tctcaaaaaa gagggcatgg cgcagcgatt
     1441 caaccgtcgc ctcgccgagc agttgcttca tctcagtaag ataccagttg atcaaataac
     1501 cggtattact ccagataagt ttgccgttga tctctccggt cgcttctagt gcctgcacaa
     1561 ccggaaccag cgcctggctg attaacgttt ccattcgctg ctgcggcgaa tgtggtgttg
     1621 cgtttttatc ttcacacaca tcgacccaga aacaggcgac gcgtccggtt tcgtgaaact
     1681 cagcatggaa atgttccggc gacacatcta atgctttttc ctgcgtcagt agcgccagca
     1741 ttaatggtgg caccatcagg ccgatatacc attgtgccca tagtgagatc agcggtttgt
     1801 actcgcggat catcgttggt tggttgcgat agatatgatc ggaatagacc gccagtagag
     1861 aacttagcgc attcggtgat gaccattgcg ccagcgtcat ggcgttaagc ggagcgggtt
     1921 catccaggcg gataaactcc agcaaatgtt cacgatgttc ggcgatcgtc gcacgtacgg
     1981 cttgtgcaag cctagcatcc tgtggctgga gatgcgttcg ccagatgata tcttcataga
//
LOCUS       DBFIFS010000381         2039 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.382, whole genome shotgun sequence.
ACCESSION   DBFIFS010000381 DBFIFS010000000
VERSION     DBFIFS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.382"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..654
                     /locus_tag="N7A07_004855"
     CDS             <1..654
                     /locus_tag="N7A07_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU2479330.1"
                     /translation="EKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGDVCKGDILTY
                     DTKNNYIYSESALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYI
                     SHREVFRPWGKFDSIDQGERYKVKKIIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLG
                     DKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            658..2028
                     /gene="cpsG"
                     /locus_tag="N7A07_004856"
                     /gene_synonym="manB"
     CDS             658..2028
                     /gene="cpsG"
                     /locus_tag="N7A07_004856"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU2479331.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
BASE COUNT          558 a          461 c          533 g          487 t
ORIGIN      
        1 gaaaaaacag aaaaatgtgt tgtatgccct gttgatattg gttggagtga cgttggatct
       61 tggcaatcgt tatgggacat tagtctaaaa tcgaaaacag gagatgtatg taaaggtgat
      121 atattaacct atgatactaa gaataattat atctactctg agtcagcgtt ggtagccgcc
      181 attggaattg aagatatggt tatcgtgcaa actaaagatg ccgttcttgt gtctaaaaag
      241 agtgatgtac agcatgtaaa aaaaatagtc gaaatgctta aattgcagca acgtacagag
      301 tatattagtc atcgtgaagt tttccgacca tggggaaaat ttgattcgat tgaccaaggt
      361 gagcgataca aagtcaagaa aattattgtg aaacctggtg aggggctttc tttaaggatg
      421 catcaccatc gttctgaaca ttggatcgtg ctttctggta cagcaaaagt aacccttggc
      481 gataaaacta aactagtcac cgcaaatgaa tcgatataca ttccccttgg cgcagcgtat
      541 agtcttgaga atccgggcat aatccctctt aatcttattg aagtcagttc aggggattat
      601 ttgggagagg atgatattat aagacagaaa gaacgttaca aacatgaaga ttaacatatg
      661 aaatctttaa cctgctttaa agcctatgat attcgcggga aattaggcga agaactgaat
      721 gaagatattg cctggcgcat tgggcgtgcc tatggcgaat ttctcaaacc gaaaaccatt
      781 gttttaggcg gtgatgtccg cctcaccagc gaagcgttaa aactggcgct tgcgaaaggt
      841 ttacaggatg cgggcgtcga tgtgctggat atcggtatgt ccggcaccga agagatctat
      901 ttcgccacgt tccatctcgg agtggatggc ggcatcgaag ttaccgccag ccataacccg
      961 atggattaca acggcatgaa gctggtgcgc gaaggggctc gcccgatcag cggtgatacc
     1021 ggactgcgcg atgtccagcg tctggcagaa gccaatgact tccctcctgt cgatgaaacc
     1081 aaacgtggtc gctatcagca aatcaatctg cgtgacgctt acgttgatca cctgttcggt
     1141 tatatcaacg tcaaaaacct cacgccgctc aagctggtga tcaactccgg gaacggcgca
     1201 gcgggtccgg tggtggacgc cattgaagcc cgatttaaag ccctcggcgc accggtggaa
     1261 ttaatcaaag tacacaacac gccggacggc aatttcccca acggtattcc taacccgctg
     1321 ctgccggaat gccgcgacga cacccgtaat gcggtcatca aacacggcgc ggatatgggc
     1381 attgcctttg atggcgattt tgaccgctgt ttcctgtttg acgaaaaagg gcagtttatc
     1441 gagggctact acattgtcgg cctgctggca gaagcgttcc tcgaaaaaaa tcccggcgcg
     1501 aagatcatcc acgatccacg tctctcctgg aacaccgttg atgtggtgac tgccgcaggc
     1561 ggcaccccgg taatgtcgaa aaccggacac gcctttatta aagaacgtat gcgcaaggaa
     1621 gacgccatct acggtggcga aatgagcgct caccattact tccgtgattt cgcttactgc
     1681 gacagcggca tgatcccgtg gctgctggtc gccgaactgg tgtgcctgaa aggaaaaacg
     1741 ctgggcgaaa tggtgcgcga ccggatggcg gcgtttccgg caagcggtga gatcaacagc
     1801 aaactggcgc aacccgttga ggcaattaat cgcgtggaac agcattttag ccgcgaggcg
     1861 ctggcggtgg atcgcaccga tggcatcagc atgacctttg ccgactggcg ctttaacctg
     1921 cgctcctcca acaccgaacc ggtggtgcgg ttgaatgtgg aatcacgcgg tgatgtaaag
     1981 ctaatggaaa agaaaactaa agctcttctt aaattgctaa gtgagtgatt atttacatt
//
LOCUS       DBFIFS010000382         2038 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.383, whole genome shotgun sequence.
ACCESSION   DBFIFS010000382 DBFIFS010000000
VERSION     DBFIFS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.383"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..207
                     /locus_tag="N7A07_004857"
     CDS             <1..207
                     /locus_tag="N7A07_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit A"
                     /protein_id="HGU2479332.1"
                     /translation="AILGTVAVILNCHHQGARSVRAVNEDSQPECQITGDRPVIKINN
                     TLWESNTAAAFLNRKSQFLYTTGK"
     gene            219..488
                     /locus_tag="N7A07_004858"
     CDS             219..488
                     /locus_tag="N7A07_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2479333.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            975..>2038
                     /locus_tag="N7A07_004859"
     CDS             975..>2038
                     /locus_tag="N7A07_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2479334.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYG"
BASE COUNT          533 a          445 c          565 g          495 t
ORIGIN      
        1 gcgatactgg gcactgtggc cgttatactg aattgtcatc atcagggggc gcgttctgtt
       61 cgcgccgtga atgaagatag tcaaccagaa tgtcagataa ctggcgacag gcccgttata
      121 aaaataaaca atacattatg ggaaagtaat acagctgcag cgtttctgaa cagaaagtca
      181 cagtttttat atacaacggg taaataaagg agttaagcat gaagaagatg tttatggcgg
      241 ttttatttgc attagcttct gttaatgcaa tggcggcgga ttgtgctaaa ggtaaaattg
      301 agttttccaa gtataatgag gatgacacat ttacagtgaa ggttgacggg aaagaatact
      361 ggaccagtcg ctggaatctg caaccgttac tgcaaagtgc tcagttgaca ggaatgactg
      421 tcacaatcaa atccagtacc tgtgaatcag gctccggatt tgctgaagtg cagtttaata
      481 atgactgagg cataacctga ttcgtggtat gtgggtaaca agtgtaatct gtgtcacaat
      541 tcagtcagtt gacagttgcc tgtcagactg agcatttgtt aaaaaaattt cgcatggtga
      601 atccccctgt gtggaggggc gactggtgaa aaatccttgc ttgtgattca ttatcgacac
      661 gggttcggtg gtaccaggcc gaactcaccg ggaggcaccc ggcaccatgc agtatacaga
      721 gattaggcat ataccaaggc ctctcatagc aggggccttt ttacatgtaa aaaagcccga
      781 gtgggttcgg gcaattgcat gagatactcg ttttaataat cgaaatcatt ttaaccagga
      841 ttcataaggc tgcgcaactg cgcgaccttt ttcgaattgc gggctgtagt ctcccttctg
      901 ccattgtcct gtaacttccg gacttcagcc cgctccttat tttactcaca atattatccc
      961 ggccgggagg attcatggca tttaaacact atgatgttgt cagggcggcg tcgccgtcag
     1021 accttgcgaa acgaataact caaaaactga aggaagggtg gcagccttat ggtagtgcgc
     1081 tgatttcgac agctggttat ggtgcggagt tcatccagcc agttgtgagt gaggggagca
     1141 tctcatcacc agaggagcca ggcaaccgtc cgacgacctc agcgccttct gttgcgccag
     1201 aatattacta tgtgatcgcg cttgctggtc agtccaatgg tatgtcatac ggtgagggac
     1261 tgccattgcc ggatacattc gacagccctg atccacgtat taaacagtta gcgcgtcgca
     1321 gtacggtgac accgggcggt gcagtatgca aatataacga catcattccg gcggaccatt
     1381 gtctgcatga tgtgcaggac atgagccgtc ttaaccatcc gaaagcggac ctgtcaaagg
     1441 ggcagtacgg aaccgtgggg caggggctgc atatcgccaa aaaactgctg ccgtttatac
     1501 cggcgaatgc gggcattctg ctggttccgt gctgtcgtgg tggttcagcg ttcaccaccg
     1561 gagccgatgg cacatacagt gacgcgagtg gtgcctcgga gaattcaacc cgctggggtg
     1621 tggacaagcc gctgtataag gaccttatcg gtcgaacaaa agcagcactg aagaagaatc
     1681 cgaaaaatgt gctgtttgcc gtggtgtgga tgcaggggga atttgatttt ggcggtacgc
     1741 cggtaaatca cgccgcacag tttggtgcgc tggttgataa attccgtgca gacctggcgg
     1801 atatggcagg ccagtgcgtc ggtggctctg ctggcggtgt tccctggata tgcggggaca
     1861 cgacgtattt ctggaagcag aagaacgaat ccacgtacca gacggtgtat ggcagctata
     1921 aaaacaaaac ggaaaagaat atccatttcg taccgttcat gacggatgag aacggggtga
     1981 atgtgccgac gaacaaaccg gaagaagacc cggacattcc gggtatcgga tattacgg
//
LOCUS       DBFIFS010000383         2017 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.384, whole genome shotgun sequence.
ACCESSION   DBFIFS010000383 DBFIFS010000000
VERSION     DBFIFS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.384"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1113
                     /gene="zorA2"
                     /locus_tag="N7A07_004860"
     CDS             <1..1113
                     /gene="zorA2"
                     /locus_tag="N7A07_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HGU2479335.1"
                     /translation="HTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDFNQNLTE
                     QFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEECKEI
                     PLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKSGAA
                     NVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSETLTS
                     TLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSSNAD
                     NMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLGNAL
                     LSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
     gene            1120..1827
                     /gene="zorB2"
                     /locus_tag="N7A07_004861"
     CDS             1120..1827
                     /gene="zorB2"
                     /locus_tag="N7A07_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HGU2479336.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            1831..>2017
                     /locus_tag="N7A07_004862"
     CDS             1831..>2017
                     /locus_tag="N7A07_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2479337.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHG"
BASE COUNT          685 a          372 c          409 g          551 t
ORIGIN      
        1 cacactcatt tcagtaataa gctttgggaa caacttgaac aatttgcaga tctaatggca
       61 aaaggtgcta cagaacaaat tattgatgct ttgcgacaag tcattattga ttttaatcaa
      121 aatttaactg aacagtttgg tgaaaacttt aaagctcttg atgcctctgt aaaaaaactt
      181 gttgagtggc agggaaatta taaaacgcaa attgagcaga tgtcagaaca atatcaacaa
      241 agtgtcgagt ccctggttga aacaaaaact gcggttgcag ggatttggga agaatgtaaa
      301 gaaattcctc tggctatgtc tgaactgcgt gaagtgcttc aggtgaacca acatcaaatc
      361 agcgaactct cccgccattt agaaaccttt gtcgccatcc gcgataaagc tacaaccgta
      421 ttacctgaaa tacagaacaa aatggctgaa gtgggtgaac tgctgaaatc cggagctgca
      481 aatgttagtg catctcttga gcaaaccagc cagcaaatac ttcttaatgc agattcaatg
      541 cgcgttgccc tggatgaagg taccgaagga ttcagacaat cggttaccca aacacaacaa
      601 gcatttgcct cgatggcaca tgatgtcagc aattcctccg aaaccctaac cagcacgtta
      661 ggtgaaacaa ttactgaaat gaaacaaagt ggtgaagaat tcctgaaatc actagagtcg
      721 cactcgaaag aattgcatag aaatatggaa caaaatacga cgaatgtgat tgatatgttc
      781 agtaagactg gtgaaaagat taaccatcaa ctatccagta atgccgataa tatgtttgat
      841 tcaatccaga catcatttga taaggcaagt gcagggctga cttctcaagt cagagaatca
      901 attgaaaaat ttgctctatc catcaacgag cagttacatg cttttgagca agcaactgaa
      961 cgtgaaatga accgtgaaat gcaatcatta ggtaatgctc tgctttcaat cagcaaaggt
     1021 tttgtcggta actatgaaaa acttattaaa gattaccaaa tagttatggg gcagttacaa
     1081 gcattaattt ctgctaataa acatcgcggg taatcgatca tggataagat tatagggaaa
     1141 caattaccta aaaaagatca agataatgaa cattgggtat ccatgtcgga cctaatggct
     1201 gggctgatga tggtttttat gttcatatct attgcttata tgcactacgt acgtattgaa
     1261 aaagaaaaaa ttaaagaagt tgccgtagcc tacgagaatg ctcagttaca gctttataat
     1321 gctctggata ttgagtttgc aaaggattta caagactggg atgcagagat cgataaacag
     1381 actctggagg ttcgatttaa atcaccggat gttttatttg gcttaggaag cacagagcta
     1441 aaaccaaagt ttaaactcat tcttgacgac ttctttcctc gctacctaaa agttctagat
     1501 aattatcagg aacatattac tgaagtccgc attgaaggtc acacaagtac tgactggaca
     1561 ggaacaacga atcctgatat tgcttatttt aataatatgg cactatcgca aggtcgtaca
     1621 cgtgcagtat tacaatacgt ttatgacata aaaaatatcg cgacacacca acaatgggtt
     1681 aaaagtaaat ttgccgcagt aggttattca tctgcacatc ccattcttga taaaaccggc
     1741 aaggaggatc ctaatcgctc tcgtcgtgtc accttcaaag ttgtaacaaa tgccgagttg
     1801 cagattagaa agattattca ggagtaagag atgaaattat ctatcgacat ttcagaactt
     1861 attcaattag ggaagaaaat gttaccagaa ggagtcgatt tttttctgga tgaatcccct
     1921 attgactttg atcctataga tattgagtta tccacgggta aagaagttag tatcgaagat
     1981 cttgaccctg gtagcgggct tatctcttat catggcc
//
LOCUS       DBFIFS010000384         2013 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.385, whole genome shotgun sequence.
ACCESSION   DBFIFS010000384 DBFIFS010000000
VERSION     DBFIFS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.385"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..165
                     /locus_tag="N7A07_004863"
     CDS             <1..165
                     /locus_tag="N7A07_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2479338.1"
                     /translation="KNESLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAA
                     FREKYHQMAA"
     gene            116..817
                     /gene="traT"
                     /locus_tag="N7A07_004864"
     CDS             116..817
                     /gene="traT"
                     /locus_tag="N7A07_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU2479339.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(865..1116)
                     /locus_tag="N7A07_004865"
     CDS             complement(865..1116)
                     /locus_tag="N7A07_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU2479340.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1140..1430)
                     /locus_tag="N7A07_004866"
     CDS             complement(1140..1430)
                     /locus_tag="N7A07_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU2479341.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          609 a          470 c          423 g          511 t
ORIGIN      
        1 aaaaatgaga gcctgagcca ctatcgtttt aagagccggg atgaggccat ctcagtaata
       61 cgggaataca ttgagatttt ctacaatcgt cagcgtcgtc actctcgtct ggggaatatc
      121 tccccggcag ccttcaggga aaaatatcat cagatggctg cttaaaaaaa gaacaaatgg
      181 tagtgtccgc tattgtcagt acacctcaac catctggctt gaacctgcga gcgaacgcac
      241 cgttttcctg cagatcaaaa acacgtcgga taaagacatg agtggactac agggaaaaat
      301 tgccgatgca gtcaaagcca gaggttatca gatcatgact tccccggata aagcttacta
      361 ctggatccag gctaacgttc tcaaagcaga caaaatggat ctgcgtgaat ctcagggatg
      421 gctgagtcgc ggttatgaag gtgcagcagt cggagctgct cttggaggtg gaatcacagc
      481 gtacaatacc gggtcggcag gtaccactct cggtgttgga ctggctaccg gtctgattgg
      541 catggccgcg gatgcaatgg tggaagatat aaattacaca atgattacag atgttcagat
      601 tgccgagcga accagaacca gcgtcagaac ggataatgtt gcagctttgc gtcagggaac
      661 atcaggctcc aaaattcaga caagcacaga aaccggcaac cagcataaat accagacccg
      721 ggttgtatcc agtgccaacc aggttaatct gaagtttgaa gaagctaaac ctcatctgga
      781 agaccagctg gcaaaatcaa tcgccaatat actctgatat aaatatcccg gtggccattc
      841 acctggcctc cggccctctt agtctcaacg ttcagacaat tcagatatta ttttccctgt
      901 cacaccatat acccatgccg gaacagaatc taatgacggt accagtaaat tcttcagcgt
      961 tcggtttgcc tgacgcccgt agctgtattt gtgtctgttt cttcttatac aagagcagta
     1021 gtacaatttc tcttccagag tcagttcaac tttaggacgt aaccaataaa gatctcttcc
     1081 actataaaat ggtgaatcct gtacaaacga ttccataaca gaacctcctg ctgctacagt
     1141 taacacagct gctggcatgg actaatacca tcttttaatt taaccctggc ggagacgcca
     1201 ttatttttgg acgttcttga aacaaaatta attcctgaag aatctttctc cagtttattc
     1261 agctcaagat tcagtccata attaacgtca aataactctg ataaaggtat cagtttatgt
     1321 tcaggataat gattatcccg acgacaacaa atattaaaat cctgcatatt acccgacatc
     1381 aggagttcaa aaccgtcccc ggcatcattc atactggcag ataaattcat tacccagttt
     1441 agtgctaatc aacaggtacg gcttaactta caggaattcc ccgggagggg cggactcatc
     1501 ctcagttagc ggcaaaatcg catcacagcc aatcgcctgt ctcgattccc catcggcgaa
     1561 caatgtatgt ggccacaaaa tcctggctgg ctcagaacga agagcatctg agcaaagagg
     1621 tcatcaatct ggtatgaata tctggcagaa aatatcccct gaatccacaa ctgctgtgca
     1681 tatctgctca aatcagcatc aatcataaac cagtaatcac agtgcctgtg attgggttac
     1741 aggaataagt cccccttcat aaggcatatc atcaataccg gtgaaggaaa agaaagcatc
     1801 agatggcctg atagtctgcg tcagtcaatg acatcagtgc tttatccgga acaatgtatc
     1861 catgttggcg acagagaaag ccattgccat gaacagtttt gtttatcccc ggaaagtcat
     1921 ttctgttaca aatttatatt gacaaacttg ctttcgatga gaaacatact atttcaatga
     1981 aatgcagaat gaggcaaaat ttcgttatgt ttt
//
LOCUS       DBFIFS010000385         1977 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.386, whole genome shotgun sequence.
ACCESSION   DBFIFS010000385 DBFIFS010000000
VERSION     DBFIFS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.386"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..436
                     /locus_tag="N7A07_004867"
     CDS             <1..436
                     /locus_tag="N7A07_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2479342.1"
                     /translation="VLDYGCTVSSDSLNFTVDLQKNSARQFPTTGSTSPAVPFQITLS
                     ECSKGTTGVRVAFNGIEDAENNTLLKLDEGSNTASGLGIEILDGNMRPVKLNDLHAGM
                     QWIPLVPEQNNILPYSARLKSTQKSVNPGLVRASATFTLEFQ"
     gene            449..952
                     /locus_tag="N7A07_004868"
     CDS             449..952
                     /locus_tag="N7A07_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2479343.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            1012..1926
                     /locus_tag="N7A07_004869"
     CDS             1012..1926
                     /locus_tag="N7A07_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2479344.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          566 a          429 c          454 g          528 t
ORIGIN      
        1 cgtcctcgat tatggctgca cagtctcatc ggattcgctt aattttaccg tagatctcca
       61 aaaaaacagt gccagacaat ttccaacgac cggtagcaca agtccagccg tcccttttca
      121 gattacgtta agtgaatgca gcaaagggac aacgggggtt cgggttgcat ttaacggtat
      181 tgaggacgca gaaaataata ctctgttgaa actggatgag ggaagcaata cggcctccgg
      241 tttaggtata gaaatactgg acggaaatat gcgtccggtg aaactgaatg accttcatgc
      301 cgggatgcag tggatcccac tggtaccaga acagaacaat attttgcctt actccgctcg
      361 tctgaagtca actcagaagt ccgtcaatcc gggactggtg agggcttcgg caacctttac
      421 ccttgaattt caataatcag agtcagatat gaaaagactt cacaagaggt tcctcttagc
      481 tacgttttgc gcgttattaa cagcaactct ccaggccgcc gatgtcacta tcactgttaa
      541 tggtcgggta gtcgctaaac cctgcactat tcaaaccaaa gaagctaacg ttaatctcgg
      601 ggatctttat acgcgcaatc tgcaacaacc tggttctgca tctggctggc acaatattac
      661 tttgtcatta accgattgtc cggctgaaac aagtgcagtg acggcaatcg tgacaggttc
      721 aactgacaat acgggttatt acaaaaatga aggtactgcc gaaaatattc agatagagct
      781 tagggatgac caggatgcga cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga
      841 gatcactcgt aatgcacagt ttccacttaa ggcaagagct atcacggtga atggaaacgc
      901 aagccaggga acgatcgagg cgctaatcaa tgtgatctac acctggcaat aacgtcgatt
      961 cgattttctt agccttcttt ttaaatcaat tttttaacct taggatatat catgggtaaa
     1021 acaatcagta taaaggtcct gttcggtatt tatcttctat taatggcagg caaagttttt
     1081 gccttcagtt gtaatgtcga tggcgggtca agtattggag ctggtacaac ctctgtttat
     1141 gttaatctcg accctgtaat acagccgggc cagaatctgg ttgtagactt gtctcagcat
     1201 ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa cctggtacaa
     1261 ggctcagctt tcgcaggctc tctacagtca tataaaggat cgctgtactg gaataatgtg
     1321 acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga taagacaccg
     1381 atgccattac cgttaaaact ctatattacc ccagtcgggg cggccggtgg agttgtcata
     1441 aaagccgggg aagttattgc ccgtattcat atgtataaaa ttgccacgtt aggaagcgga
     1501 aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat gccaacaggc
     1561 ggctgcactg ttgatagtcg taatgtcacc gtgaatctcc cggactttcc tggaagcgca
     1621 gaaatccccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta tctttcgggt
     1681 actaccactg acagtgctag acaagtgttt gctaacacag caccagatgc aacaaaagca
     1741 tcgggagtgg gtgtctctct gatgcgtaat ggaaaaatac tggctacggg agaaaatgtc
     1801 tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac ctatggacaa
     1861 acagggaaca aagtttcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat
     1921 gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg cgcggat
//
LOCUS       DBFIFS010000386         1968 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.387, whole genome shotgun sequence.
ACCESSION   DBFIFS010000386 DBFIFS010000000
VERSION     DBFIFS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.387"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            132..233
                     /locus_tag="N7A07_004870"
     CDS             132..233
                     /locus_tag="N7A07_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479345.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            230..685
                     /gene="ybcN"
                     /locus_tag="N7A07_004871"
     CDS             230..685
                     /gene="ybcN"
                     /locus_tag="N7A07_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGU2479346.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQQLRDKQEA"
     gene            685..855
                     /locus_tag="N7A07_004872"
     CDS             685..855
                     /locus_tag="N7A07_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2479347.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            848..1138
                     /locus_tag="N7A07_004873"
     CDS             848..1138
                     /locus_tag="N7A07_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2479348.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1135..1497
                     /gene="rusA"
                     /locus_tag="N7A07_004874"
     CDS             1135..1497
                     /gene="rusA"
                     /locus_tag="N7A07_004874"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU2479349.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1494..1634
                     /locus_tag="N7A07_004875"
     CDS             1494..1634
                     /locus_tag="N7A07_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2479350.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1720..>1968
                     /locus_tag="N7A07_004876"
     CDS             1720..>1968
                     /locus_tag="N7A07_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2479351.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMA"
BASE COUNT          506 a          460 c          515 g          487 t
ORIGIN      
        1 gcttccagag agtaaaagtg ttttaagaag gagcaattct atcgattttg attttgggaa
       61 atcaacacgg cataattatg tcaccggagc ctgaacaact ccggtgactt ctgcgctaaa
      121 cggggacgtt tatgcgcaca tacaatccaa actctcttct cccttcacag atgcagaaat
      181 gcacctgcaa ttctttgcat ctagcgtttg acctctgcgg aggtgaagcg tgaacctctc
      241 acaagacggc atcaaattac atcgcggcaa cttcaccgct atcggtcggc agatccagcc
      301 ttatctggag gagggcaaat gctttcgcat ggtgcttaaa ccgtggcgtg agaaacgcag
      361 tctttcccag aatgcactca gccacatgtg gtacagcgaa atcagtgaat acctcatcag
      421 caggggtaaa acgttcgcca ctccagcttg ggtaaaagat gctctcaaac acacatatct
      481 cggttatgaa accaaagacc tggttgatgt cgtaaccggt gatatcacca ctatccagtc
      541 gttacgccat acctccgatc ttgataccgg agagatgtat gtcttcctgt gtaaggttga
      601 agcctgggcg atgaatattg gttgccatct gactattccg cagagctgcg agttccagca
      661 gctccgtgac aagcaggagg cgtaatggct acaccgctta ttcgtgtcat gaacggacac
      721 atctacagag tatcaaatcg tcgtaagcgt aagcctgagc tgaagccatc cgaaatacca
      781 acactgctcg gatataccgc tagcctggtt gataaaaaat ggttgcgact ggcagcaagg
      841 aggaatcatg gctgatttga gaaaagcagc gcgtggtcgg gaatgccagg taagaatccc
      901 tggcgtatgt aatggcaatt ctgaaacgtc tgtactggca catatccggc tggctggatt
      961 gtgcggtacc ggtatcaaac cgccagacct gattgccacc attgcatgtt ctgcctgcca
     1021 cgacgaaatc gaccgccgca cacattttgt cgatgctgca tatgcaaaag aatgcgcgct
     1081 ggaaggtatg gcgagaacac aggttatctg gctgaaagag ggggttatta aggcgtgaat
     1141 acctacagta tcacattacc ctggcctccg agcaataatc gctattaccg ccataatcgc
     1201 gggcgcacgc acgtcagtgc agaggggcag gcataccgcg ataacgtcgc ccgaatcatt
     1261 aaaaacgcaa tgctggatat cggcctggct atgcctgtga aaatccgcat tgagtgccac
     1321 atgccggatc gccgtcgccg tgacctggat aatctgcaaa aagccgcttt tgacgcactc
     1381 actaaagcag gtttctggct ggatgatgct caggtcgttg attaccgcgt tgtgaagatg
     1441 cctgttacca aaggtgggag gctggaactg accatcaccg aaatggggaa tgaatgatgt
     1501 ttgagtttaa tatggcagaa cttcttcgcc accgctgggg gcgtctgcgc ttatatcgtt
     1561 tccccggttc tgttttgacc gattaccgaa tactgaagaa ttacgccaaa accctgacag
     1621 gagcaggagt atgaagtcag agataacaat caactaatac tgttttgttg atttttgctt
     1681 gtaattggcg ttctggtctg atttttgtgg agtaagttga tgcgtgatat tcagatggtt
     1741 cttgagcgtt ggggagcgtg ggcggctaat aatcatgaag atgtgacctg gtcgtccatt
     1801 gccgccggtt ttaagggact gatccccgaa aaagtaaaat cacgcccgca gtgctgtgac
     1861 gatgatgcga tggtgatatg cgggtgtata gcccgccttt accggaacaa tcgcgatctg
     1921 catgacttgc tggttgatta ttacgtgttg ggggagacgt tcatggcg
//
LOCUS       DBFIFS010000387         1963 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.388, whole genome shotgun sequence.
ACCESSION   DBFIFS010000387 DBFIFS010000000
VERSION     DBFIFS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.388"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..154
                     /locus_tag="N7A07_004877"
     CDS             <1..154
                     /locus_tag="N7A07_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2479352.1"
                     /translation="YWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYLTNLYFKIREDRR
                     KVARGE"
     gene            complement(410..682)
                     /locus_tag="N7A07_004878"
     CDS             complement(410..682)
                     /locus_tag="N7A07_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479353.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            842..1375
                     /locus_tag="N7A07_004879"
     CDS             842..1375
                     /locus_tag="N7A07_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2479354.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1596..1709
                     /locus_tag="N7A07_004880"
     CDS             1596..1709
                     /locus_tag="N7A07_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479355.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1711..>1963
                     /locus_tag="N7A07_004881"
     CDS             1711..>1963
                     /locus_tag="N7A07_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU2479356.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDHAIIYKEQRDKKASEL
                     ELANATITDMQIRQRDVAALDARYSRELADARAENETLRA"
BASE COUNT          515 a          384 c          536 g          528 t
ORIGIN      
        1 atactggtta ctgcagctgc tggacaaagt ctctccgtcc cagtgggtgg caataggtgt
       61 gctgggaagt ctgctgtttg gcctgctgac gtatctgact aacctgtatt tcaaaatcag
      121 agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg aataaacaat acgaactggt
      181 tgtaaaatga atatttctaa ctgaaaaaac gttccatgag gtaagaaaag gtcacaggca
      241 atcaataaca ggacgtgatg aaagaccctt gcatttgtgc gctttctctt tagatagcag
      301 cagatactga aaatctgagt tgtcggggag tcagggatac agctgtgcaa gagttggtca
      361 ttgtgattcc attgaaatcc tgtatgccat gaagggcagg attttatggc tacctgagct
      421 ttggtgatag taagttgaaa attcgcattt tttgctgaca tgcgtaacga gaatcccata
      481 agcagggagg acttaattct tcattaaccc atgcgttgat attatgtttc agccgttgaa
      541 gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt tttatgtgag atcttaccgt
      601 aagggtctgc aagaatgctg cttgttgctt cgttattatc tgccatcaga agaagtaact
      661 ctgatttaac gttttctgtc attagttgta aaaatcttct gcgcaaactt tctttactgt
      721 tcatttatat ggcttcattt gttgtaatct gctgcgtctc aagggatatg tttatgagag
      781 cgaccatgag tgttggatta tatacctaac atatcaaggg attagaaatc gataaatccc
      841 catgaacgaa aaaataaaat acggcctgtc ggctgccgtt ctggcgctga ttggtgcagg
      901 tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa aaggaaggta accacaccac
      961 ggcataccgt gatggtgcgg ggatctggac catctgccgt ggtgccattc tggtggatgg
     1021 taagcctgtt attcctggca tgaagctgtc aaaggaaaaa tgcgaccggg ttaatgccat
     1081 cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc cgggtgccgc tgaccgaacc
     1141 ccagaaagcg gggatcgcgt cattctgtcc gtacaacatt ggccccggta agtgcttccc
     1201 gtcgacgttt tataaacgaa ttaatgcagg cgatcgaaaa ggtgcctgtg aggcgattcg
     1261 ctggtggatt aaggacggtg gcagagactg ccgtatccgt tcaaataatt gctacggtca
     1321 ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg gacatcgaca gatagcagaa
     1381 tattttcctg aaaaatgacg ttggccaacg cgggtggata acacgaaatc ctgaaaactg
     1441 gtaaaaccta agtgaataaa agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg
     1501 ttttctgacc ttgagtaagg caagggagaa attatgggta gggaggtact ttccctgtga
     1561 ggaagtataa aagattcttt ctgaggttgt ccattatgaa aggcattgaa gtggagacgc
     1621 cagccagtct ggatttaaca agagcggcag cttttgccat tcgtattgtg gccattgctg
     1681 ttctggtctg ggcaatccgt tggtggtgat atgaaccgtg ttctgtgtgt ggttatcatt
     1741 gtcctgctgg tggcctgtgg tgcgcttagt ctggggctga atcattaccg tgatcacgcc
     1801 atcatctaca aagagcagcg cgataaaaaa gccagtgagc tggagctggc gaacgcgaca
     1861 attactgata tgcagatacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1921 gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctg
//
LOCUS       DBFIFS010000388         1962 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.389, whole genome shotgun sequence.
ACCESSION   DBFIFS010000388 DBFIFS010000000
VERSION     DBFIFS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.389"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..315
                     /gene="ehxC"
                     /locus_tag="N7A07_004882"
                     /gene_synonym="hlyC"
     CDS             <1..315
                     /gene="ehxC"
                     /locus_tag="N7A07_004882"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGU2479357.1"
                     /translation="KYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMELYKYMRKKYP
                     YELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNRQDFNFNIEK
                     EN"
     gene            317..>1962
                     /gene="ehxA"
                     /locus_tag="N7A07_004883"
     CDS             317..>1962
                     /gene="ehxA"
                     /locus_tag="N7A07_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU2479358.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQRAGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSG"
BASE COUNT          651 a          336 c          456 g          519 t
ORIGIN      
        1 aaatatataa atgatgtaac atctttgtat gcaaaagact ggatgtcggg agaaagaaag
       61 tggtttattg actggatagc tccatttgga cacaacatgg agttatataa atacatgagg
      121 aaaaagtatc cttatgagtt attcagagca ataaggttag atgagagcag taaaacagga
      181 aaaatagctg aatttcatgg aggggggata gataaaaaac tggcatcaaa aatattcagg
      241 caataccatc atgaacttat gtctgaagta aaaaacagac aagattttaa ttttaatatt
      301 gagaaagaaa actaatatga cagtaaataa aataaagaac attttcaata atgcgacatt
      361 gactacaaaa tcagcattta atacagcatc atcaagcgta cgttccgctg gaaaaaaact
      421 catattatta atacctgata attatgaagc tcagggcgtg ggtattaatg agttggtcaa
      481 agctgctgat gagcttggaa tagaaataca ccgtactgaa cgagatgata cagcgattgc
      541 aaaccagttt tttggtgcag cagaaaaagt tgtaggatta actgaacgtg gtgttgcaat
      601 attcgcacca caacttgaca aacttctgca gaagtatcag aaagttggga gtaaaatagg
      661 aggaaccgct gaaaatgtag gtaataatct gggaaaagcc ggaacagttc tctcagcact
      721 acagaatttt acggggattg ctttatcagg catggctctt gatgaattgc tgagaaaaca
      781 acgggcagga gaggatataa gtcagaatga tattgccaaa agtagtattg aacttattaa
      841 tcagcttgta gatacagtat caagtataaa cagtaccgtt gattcatttt ctgagcagct
      901 taaccagctt ggctcatttt tatccagtaa acctcgatta agttctgttg gtgggaaatt
      961 acaaaattta ccagacctgg gccccctggg ggatgggctg gatgttgtct ccggaattct
     1021 ttctgctgta tcagcaagct ttattctggg aaacagtgac gcacatacag gaacaaaagc
     1081 tgcagcgggt atcgaactga caactcaggt tcttggaaat gttggtaaag ctgtttcgca
     1141 atatattctg gctcagagaa tggcacaggg gttatcgaca acagctgcaa gtgcgggtct
     1201 gatcacatcg gctgttatgc tggctatcag tcctctttct ttcctggctg ctgcagataa
     1261 atttgagcga gctaagcagc ttgaatcata ttctgaacga tttaaaaaat tgaattatga
     1321 aggggatgct ttactcgcag cctttcataa agaaaccgga gctatagatg cagccctgac
     1381 aacaataaat actgtcctga gttctgtatc tgcgggagtt agtgcagcct ccagtgcatc
     1441 cctcataggg gccccgataa gcatgctggt gagtgcatta accggtacga tatctggcat
     1501 tctggaagca tcaaaacagg ctatgtttga gcacgttgca gagaaattcg ctgctcggat
     1561 caatgaatgg gaaaaggagc atggcaaaaa ttattttgag aatggatatg acgcaagaca
     1621 tgctgcgttt ttagaagact ctctgtcttt gcttgctgat ttttctcgtc agcatgcagt
     1681 agaaagagca gtcgcaataa cccagcaaca ttgggatgag aagatcggtg aacttgcagg
     1741 cataacccgt aatgctgatc gcagtcagag tggtaaggca tatattaatt atctggaaaa
     1801 tggagggctt ttagaggctc aaccgaagga gtttacacaa caagtgtttg atcctcaaaa
     1861 agggaccata gacctttcaa caggtaatgt atcaagtgtt ttgacattta taacaccaac
     1921 atttacccca ggagaagaag ttagagaaag aaaacagagt gg
//
LOCUS       DBFIFS010000389         1926 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.390, whole genome shotgun sequence.
ACCESSION   DBFIFS010000389 DBFIFS010000000
VERSION     DBFIFS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.390"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            159..773
                     /gene="evgA"
                     /locus_tag="N7A07_004884"
     CDS             159..773
                     /gene="evgA"
                     /locus_tag="N7A07_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU2479359.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            778..>1926
                     /locus_tag="N7A07_004885"
     CDS             778..>1926
                     /locus_tag="N7A07_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2479360.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAE"
BASE COUNT          605 a          388 c          369 g          564 t
ORIGIN      
        1 tgacatttca tatatgccga ctatttatat ggtatacttg tcgaattatc ttaaaggaag
       61 ctcaaatttt cttattttta ttgagaaaat gagatgatgc cttatgtctg tattactaca
      121 gggagaaggg agatgcttca ttgcaaaggg aataatctat gaacgcaata attattgatg
      181 accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat attgaaatct
      241 tagcagagtt gactgaaggc ggaagtgccg ttcagcgggt ggaaacactt aagcctgata
      301 tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta gaaacgctga
      361 ggaagcgcca atatagcgga attattatta tcgtctccgc taaaaatgac catttttacg
      421 ggaaacattg tgctgatgct ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca
      481 atatcattgc ggctattgaa gctgcaaaaa atggctactg ctatttcccc ttctctctca
      541 accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta tcgaaacaag
      601 aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt gctgaaaaaa
      661 tgttcatcag caacaaaact gtcagcactt ataaaagtcg cctgatggaa aaattagaat
      721 gtaaatcact gatggatctt tacacattcg cacaacgtaa caaaatcggc taaccacatg
      781 aagtttttac cctatatttt tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca
      841 gacgaagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcac acttgatcca
      901 ctacgtctga gcaacaagga attacgttgg ttagcgagca aaaaaaatct tgtgattgca
      961 gtacacaagt cccaaacggc tacgttgttg cataccgatt cgcagcaacg ggttcgtggt
     1021 attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt aacactccgg
     1081 gaatacgcag atcatcaaaa agcaatggac gcgctggaag aaggtgaagt cgatatagtg
     1141 ttatcacatt tagttgcttc gccgcctctt aatgatgaca ttgctgcaac caaaccactg
     1201 ataattacct ttccggcgct ggtcaccacc cttcacgatt caatgcgacc gcttacctca
     1261 tcaaaaccag taaatattgc tcgagtagca aattatcccc ctgacgaggt aattcatcaa
     1321 tcatttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt agcatccgtc
     1381 tcagccggac agaatgatta ctttattggt agtaacatca ttaccagcag tatgatttcc
     1441 cgctatttca ctcactcctt aaatgtagtg aaatattata actcaccgcg tcaatataac
     1501 ttcttcttaa ccagaaaaga atctgtcatt cttaatgaag tactcaatcg atttgttgat
     1561 gctttaacaa atgaagttcg ctatgaagta tcacaaaatt ggcttgatac aggaaacctg
     1621 gcctttctga acaagccatt agaactcact gaacatgaaa aacagtggat taagcagcat
     1681 cccgatttaa aggtgctgga aaatccttac tcgcccccct attctatgac ggatgaaaat
     1741 ggctcggttc ggggcgttat gggggacatt cttaatatta ttaccttgca aacaggttta
     1801 aatttttctc cgatcaccgt ttcacacaat atccatgctg gaacacagct gaatcccggc
     1861 ggatgggata tattacccgg tgctatttat agtgaagata gagaaaataa tgttttattt
     1921 gctgaa
//
LOCUS       DBFIFS010000390         1914 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.391, whole genome shotgun sequence.
ACCESSION   DBFIFS010000390 DBFIFS010000000
VERSION     DBFIFS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.391"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1914
                     /gene="espP"
                     /locus_tag="N7A07_004886"
     CDS             <1..>1914
                     /gene="espP"
                     /locus_tag="N7A07_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU2479361.1"
                     /translation="STLKLPEVPFPDFSPVANKGSTTSIGGAYSITATHNTKNHHSVA
                     TQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGATEGADISLSKQQALERYGVNYK
                     GEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFD
                     EFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLK
                     NKYSYNVDMSGAQVATIENGKLTGTGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGL
                     VFNDKKTYRVNGDDFTFKGAGVDTRNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNT
                     NLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNG
                     YNQSFNKIAATDSGAVITNTSTKKSILSLNNTADYIYHGNINGNLDVLQHHETKKENR
                     RLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGM
                     QNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFGTLHLENSDFSVGRNANVIGDI
                     QASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIV
                     IKDKAKALFSNYVY"
BASE COUNT          623 a          388 c          414 g          489 t
ORIGIN      
        1 tccaccctga aactaccgga agtaccattt cctgacttct caccggttgc aaacaaaggg
       61 tcaaccacat ctattggtgg tgcatacagt atcacagcca cacacaatac gaaaaaccac
      121 cactcagttg cgacgcaaaa ctgggggaac agcacgtaca aacaaactga ctggaatact
      181 tcacatcctg attttgcagt atcccgactt gacaagtttg ttgttgagac ccgaggtgcg
      241 actgaaggcg cagatatttc gttatcaaaa cagcaggcac ttgaacgtta cggggttaat
      301 tataaaggag aaaagaaact tatcgcattc agagccggct ctggtgtggt atccgttaaa
      361 aaaaatggac gcataactcc atttaatgag gtttcttata agccagaaat gttaaatggc
      421 tctttcgttc acattgatga ctggagtgga tggctgatat taaccaacaa ccagtttgat
      481 gagtttaata acattgcctc tcagggtgac agcggttcag cactgttcgt ctatgataac
      541 caaaagaaaa agtgggttgt cgctggaact gtctggggga tttataatta cgccaatggc
      601 aaaaaccacg cagcatacag taaatggaac cagacaacca ttgacaacct gaagaacaag
      661 tattcttaca acgtggatat gtcaggggct caggttgcaa ccattgaaaa tggaaaactg
      721 acaggcactg gctcagacac caccgatata aaaaataagg acttaatatt tactggcggt
      781 ggagatatcc tcctgaaatc ctcttttgat aatggtgctg gcggtcttgt ctttaatgat
      841 aaaaagacct atcgagtaaa cggggatgat ttcaccttta aaggtgccgg tgttgataca
      901 agaaacggca gcaccgttga gtggaatatc cggtatgata ataaagacaa ccttcacaaa
      961 attggtgatg gcacattaga tgtccgaaaa acccagaaca ccaacctgaa aacaggtgag
     1021 ggtcttgtca ttcttggagc tgaaaaaaca ttcaataata tctacataac cagtggtgat
     1081 ggaactgtcc gactgaatgc agaaaatgca ctgtctggcg gtgaatacaa cggtattttc
     1141 tttgcgaaaa atggcggaac tcttgacctg aacggatata atcagtcttt caataaaatt
     1201 gctgcaactg attcaggtgc tgtaataacc aatacgtcaa ccaaaaaatc cattttatcc
     1261 ctgaataata ctgctgacta tatctatcac ggcaacataa acgggaatct ggacgtactt
     1321 cagcatcatg agacgaaaaa agagaaccgt cgtcttattc ttgatggggg cgtggacaca
     1381 acaaatgata taagcctgcg taatacacaa ctgtccatgc agggacatgc cactgaacat
     1441 gccatttatc gggatggagc tttctcttgt tcactaccag ctcctatgcg ctttttgtgt
     1501 ggcagtgatt atgttgcagg aatgcaaaat acagaagctg atgctgtaaa acaaaacgga
     1561 aatgcctata aaaccaacaa tgctgtctct gatttatcgc agccagactg ggaaaccgga
     1621 acattcagat ttggaacgct acatcttgaa aattccgatt tttctgttgg tcgtaatgca
     1681 aatgtaatcg gggacattca ggccagtaaa tcaaacatta ctattggtga cactacagca
     1741 tatattgatt tgcatgctgg taaaaatatt accggtgatg gttttggctt ccgccagaat
     1801 attgtgcgtg gaaactcaca aggagaaacg ctgtttacag gagggatcac agcagaagac
     1861 agcactatcg ttattaaaga taaagcaaaa gcattatttt caaattatgt atac
//
LOCUS       DBFIFS010000391         1907 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.392, whole genome shotgun sequence.
ACCESSION   DBFIFS010000391 DBFIFS010000000
VERSION     DBFIFS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.392"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            137..1429
                     /gene="sctN"
                     /locus_tag="N7A07_004887"
     CDS             137..1429
                     /gene="sctN"
                     /locus_tag="N7A07_004887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HGU2479362.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1419..1877
                     /locus_tag="N7A07_004888"
     CDS             1419..1877
                     /locus_tag="N7A07_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU2479363.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
BASE COUNT          620 a          349 c          455 g          483 t
ORIGIN      
        1 cgttttatca aaaaacttat tgaatctagt tatcgagacc tcgaagtact ttcgtacggg
       61 gaactaacgg aaaatgttcc cgttaatatt ctgaaaacta tttaggataa tgtcttgcga
      121 tatggagcat gctggtatga aaaaattaaa attattaaat aaatacagct acttacatag
      181 cataaacggc tctctgattg aagcagaact tgacgacgtt tctgtgggtg aagtatgcga
      241 aatttatgcg agcaggcaag caaatgaacg tatagccagg gctcaggtcg ttggctttcg
      301 caatgggaag acattattaa accttatagg cagctctgtg gggcttacac gtactgcagt
      361 tttgaaacca acgggagagc agttaaccat acagatcagt gatgcctttc ttggttcggt
      421 tttgaatgcc tctggtcaaa ttatggaaag gtttgtcccg gacactccag gcgatagggg
      481 aaatttacgc ctgattgatg aactgccacc gtcgtatcag gaacgtcgag ttattaatac
      541 accacttgag acacggataa gagtcattga tggcgtactg acatgtggca ttggtcaacg
      601 tgttgggatt ttcgcctcgg ctggctgtgg caaaacggtt ttaatgcata tgctcgtaaa
      661 caatactgaa gctgatgtgt ttgttattgg cttgattggc gaacgtggta gggaagttac
      721 ggaatgcgcg gaatcgctca aaaaatcagt aaatgcagcg aaatgcgtat tggtctatgc
      781 cacctctgat ttttcgtcag tagaccggtg taatgccgcc ctgatggcga caaccgtggc
      841 agagtatttt cgagatcgag gaaagagagt tgtacttttt atcgactcaa tgacgcggta
      901 tgcacgagca ctacgtgata tgaaactggc agcgggtgaa ccacctgcca gacgtggtta
      961 tcctgcttct gtttttgaca gtttgcctcg cttactggag aggccgggac caacactaaa
     1021 aggaagtatt accgagtttt ataccgtgtt actggaaggg gaagatgaat cagacccact
     1081 gggtgacgaa attcgctcaa tccttgatgg ccatatctat ctaagtcgca aactcgcggg
     1141 tcaaggtcac tatccggcaa tagatgttct aaaaagtgtg agtcgagtat ttggtcaggt
     1201 cactgatgaa aaacacagag ataatgctgc acgggtaagg aagaacctca caactttgga
     1261 agatctacag gtgtttattg acttagggga atatcgcgca gggcaaaatg ctgaaaatga
     1321 ttttgctatg aatgcccggc ctaaactcac aaattggttg aaacagtcag ttaatgaaaa
     1381 aatgccaatg agtgaaacgc taaaagagct tgaacgaatt gttaagtaaa gttaatcgac
     1441 ttatcagaag aacagcgcag agcctggctg catgtgaagc atcattacaa aagttaaatg
     1501 cagaaaaaga aaagttagcc gaaaaggaac gcctgtatga tatgcaactt aaaaacctgc
     1561 agtcactgtt agatatgaag gagttactcg gcgaagttgt atttcgccag gatatatttt
     1621 actcgttacg taaagttaca gtcattcagc agcagatcgc tgagataaat cttgaaaagc
     1681 aaaaaatagc cgagcggcga aaaatactta acaaggaaat agttcaacag caagcacaaa
     1741 gaaaacactg gtggctaaaa ggagaaaaat acgaccgact aaaaaagcgc atcaaaaaac
     1801 aacttttaaa tcagatgctt tatcaagacg agcttgaaca ggaggaaaaa tataatggac
     1861 gaagtcaaga aaattgaaca ttgtaaaatt ttaaacacag acacacc
//
LOCUS       DBFIFS010000392         1907 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.393, whole genome shotgun sequence.
ACCESSION   DBFIFS010000392 DBFIFS010000000
VERSION     DBFIFS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.393"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..322)
                     /locus_tag="N7A07_004889"
     CDS             complement(<1..322)
                     /locus_tag="N7A07_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2479364.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVP"
     gene            complement(333..1031)
                     /locus_tag="N7A07_004890"
     CDS             complement(333..1031)
                     /locus_tag="N7A07_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2479365.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1031..1360)
                     /locus_tag="N7A07_004891"
     CDS             complement(1031..1360)
                     /locus_tag="N7A07_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2479366.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVCFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1357..>1907)
                     /locus_tag="N7A07_004892"
     CDS             complement(1357..>1907)
                     /locus_tag="N7A07_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2479367.1"
                     /translation="GAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGG
                     ASASSGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYR
                     LMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMAR
                     KGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          397 a          689 c          442 g          379 t
ORIGIN      
        1 gcggcacaca gcggaatttg tgaatttccc cccggcagac cagccaccag ggcagggcac
       61 tctttatctg cagccgccgg tcggcctcgc tcagccaggg cagaccacca gggtggctgt
      121 gaaccagcgc cacaatctcc ccctgcatct ctgcctgcag ccagtcttcc ggcgcaatac
      181 gaaaatacgc ctccggctct gcggagatat tcacgcaggg ctggtaccgt tcgccctccg
      241 gggtgcctat cacgaagccg cacgactccg caggcgcaca ccgccgggca tgcaccagaa
      301 tcgctgattc agtctgtgtc ataaaacagg atttactgcg aaagtttatt gatggaaagg
      361 aaaccgccaa aattagccac catgccgcgc atctcacacc cgcgcatgca tttactgcat
      421 ctgtccttcc ggatatccgt ggtggggttg tcgaactcat ccgccactgc cggaccgtta
      481 tacccgcatt catcgccccg gtaatcccac atacaggtgt tcgccagcat gatgcgaccg
      541 ggaaacagcg ccccgtccgt ctccgtcggt gtcgccagca caaacgaggc cgtcatggcc
      601 gtcagcgatg acatctgctc caccacccac cggtccgtca gctcctgctc agggtctgcc
      661 tcaggattgc ctgccacaaa attcaccgca tccagaaaac gggcatacac ccggcggcgg
      721 accaccgtgg cccccaccag gctctgcagg tcctccgcca tcccggtgac cagaccaaac
      781 agattcgaca ccgtcagcga cggtctggca ctgctgccct tcccgttcat ctcaaagcca
      841 ctgccgtcaa tcgggtatgc ctgatattgc cgcccctgcc aggtaaccgc ctcccctttt
      901 tcattcagct cattgcagaa aaaataccgc tcaccgccct gcgccgtcag gtcgatttcc
      961 cagagtacca cccgcggtga ctgctctgat ttaaccgact cgttcagact ttcttcgtga
     1021 atatcctgca tcagttcacc acctgcttaa actccgcgct gaactcaacg cgcaacatcc
     1081 cgacccgcgc agaccacccg gcacaggtca cctttatctg ccggtatgca tagggtggct
     1141 tccacaaaaa tgccttccag cctccgtgct ctgccaggaa tgcctccaga tgtcgggcct
     1201 cctcccgggt cacggaaagc gtcacacggt atgttttcag gtcagcattc agccctgccg
     1261 ccatacgctg tgagtacccg tcaccaaaac acacttcacg caccgatggc tgcgagttca
     1321 cctccatatc cggcttcact ttccagcgaa aggttttcat cgcccgctcc ccgataacat
     1381 accgccatca cgcaactgca gccggagctc atcctgcgcc cccttgcggg ccatgtcata
     1441 caccgccttc atcagctgcg gccccgcctg tccgttggtg ccgtcgttct gaatcaccac
     1501 gtgattgttc tgattaaaat taataccttc cgcccgccgc atctgcgccg gacttccggc
     1561 accacccaca taaccacctt ccgcatagcc gcgcatcaga cggtaaagat tccccacacc
     1621 tatccggctg gttgcctctt tcgtgaaaac aaactccccg cggtgaacaa ttcccgcagg
     1681 ctcatatttg ccgcccgtcc ccgtaaatcc tccggtcgcg aaatggaagt tcgccgccgc
     1741 agcctgaatg gccgtccccg aggaagcaga tgcaccacca ccgaaagcac cgccaatggc
     1801 gctgccgata cgcccgacaa tgcccaccat ggcctgttta agcaggattt ctgtcatcat
     1861 ggacagcacc gaacgggtga atccccgcca gtctgcctct gcaccgg
//
LOCUS       DBFIFS010000393         1891 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.394, whole genome shotgun sequence.
ACCESSION   DBFIFS010000393 DBFIFS010000000
VERSION     DBFIFS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.394"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..933
                     /locus_tag="N7A07_004893"
     CDS             <1..933
                     /locus_tag="N7A07_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2479368.1"
                     /translation="RLFCEGGVRPPLEKIMPLLDKLREQYGVGPVCSELHIAPSTYYH
                     CQQQRHHPDKRSARAQRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVA
                     RLMAVMGLAGVLRGKKVRTTVSRKAVSAGDRVNRQFVAERPDQLWVADFTYVSTWQGF
                     VYVAFIIDVFSGCIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLGRLGHIPPAEAEKAYYASIRNDDLAA"
     misc_feature    1..117
                     /locus_tag="N7A07_004893"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            973..1344
                     /locus_tag="N7A07_004894"
                     /pseudo
     CDS             973..1344
                     /locus_tag="N7A07_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
     gene            complement(1391..1732)
                     /gene="symE"
                     /locus_tag="N7A07_004895"
     CDS             complement(1391..1732)
                     /gene="symE"
                     /locus_tag="N7A07_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU2479369.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT          478 a          430 c          532 g          451 t
ORIGIN      
        1 cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaataatgcc gctgctggat
       61 aagctgcgtg agcagtacgg ggtcggaccg gtatgcagtg aactgcatat tgccccgtca
      121 acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc ccgtgcgcag
      181 cgcgatgact ggctgaagag agagatacag cgcgtatacg atgaaaatca tcaggtgtac
      241 ggtgtgcgta aagtctggcg ccagttgtta cgcgaaggta tcagggtggc cagatgtaca
      301 gtggcgcgcc tcatggcggt tatgggactt gccggtgttc tccggggtaa aaaggtccgt
      361 actaccgtca gccggaaagc cgtttccgca ggcgaccgcg taaaccgtca gttcgtggca
      421 gaacgtcctg accagctgtg ggtggctgat tttacttacg tcagcacatg gcagggcttc
      481 gtctatgtgg cgttcatcat tgatgtgttt tccggatgta tcgtggggtg gcgagtctca
      541 tcgtctatgg aaacgacatt cgtgctggat gcactggagc aggcgttgtg ggcccgtcgg
      601 ccgtccggca cagtccatca cagtgataaa ggttctcagt atgtatcact ggcctatacg
      661 gagcgactaa aagaagcaaa actgctggca tcaacaggga gtacaggtga ctcgtatgac
      721 aacgcgatgg cggagagcat caatggtctt tacaaagcgg aggtaataca ccgtaagagc
      781 tggaaaaacc gtgcagaagt ggaactggcc acactcacgt gggtggactg gtataacaat
      841 cgacgattgc tgggaaggct gggtcatatc cctccggcag aagcagaaaa agcttattat
      901 gcttccatca gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
      961 aaccggggcg gttcatacag cctgagtgat tatccttttc ttgctgatgg ttcgcatagc
     1021 cgggtgttat tcgatcatct tcgcgatgaa attatgcgcc tggacgcagg gatcacgcag
     1081 gaagtattga agctctatat tgcgtttaaa gcggaaacga attttgttga tgttgtgccg
     1141 caaaaaagcc gactgcgatt gtcgcttaat atgcagtttc atgaactggt cgatccgaaa
     1201 ggtattgcca aagatgtgac aaatgttggg cgatggggca atggtgatgt ggaaattggt
     1261 ttcagtgacc tcgcacaact tccttacatt atggggttaa ttcgtcaggc atttgaaaaa
     1321 cagatggaga gcgcgttggt ataacgcagc gaaaaatgcc cggtagtcct ggcgctaccg
     1381 ggtcaatgta tcaggcaact ttctgtttac ccgcaatcac cccaataaac tcctgcacct
     1441 gcctttgttt acgcgccgac agcttgcaca cctggcgcag cgactgcatc agttcgctct
     1501 cggcggcggc gggtggttgg gcggtgagga caatacagcc ttccatcact ttgacatcta
     1561 ccaccgtgcc ggtggcaaaa ccggcggctt ccagccactg acctttcagg gtgatggcgg
     1621 gaatacggct gtaatccggg taacgactcg cataactcac ggttaattga cggttatttg
     1681 ccggggagac ttctgcttcg aacggttgtg caatagaatg cgtatcagtc ataactgcta
     1741 ttccctataa atagtgattg tgattagcgg tgcgggtgtg ttggcgcaca tccgcaccgc
     1801 gctaaatacc tggatatatc atcagtaaat atggggaaag tccagctaaa aattaatgaa
     1861 aataagatga aaattagcgt gaaatataaa t
//
LOCUS       DBFIFS010000394         1830 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.395, whole genome shotgun sequence.
ACCESSION   DBFIFS010000394 DBFIFS010000000
VERSION     DBFIFS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.395"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            35..130
                     /gene="mgtS"
                     /locus_tag="N7A07_004896"
     CDS             35..130
                     /gene="mgtS"
                     /locus_tag="N7A07_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU2479370.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            130..234
                     /gene="mgtT"
                     /locus_tag="N7A07_004897"
     CDS             130..234
                     /gene="mgtT"
                     /locus_tag="N7A07_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU2479371.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(349..1239)
                     /gene="dgcZ"
                     /locus_tag="N7A07_004898"
     CDS             complement(349..1239)
                     /gene="dgcZ"
                     /locus_tag="N7A07_004898"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU2479372.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1494..>1830)
                     /gene="ydeI"
                     /locus_tag="N7A07_004899"
     CDS             complement(1494..>1830)
                     /gene="ydeI"
                     /locus_tag="N7A07_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU2479373.1"
                     /translation="DDQGGLKQDAAPPPPHAIEDGYRGTDDAKKMTVDFAKTMHDGAS
                     VSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREVQPDQMINISGSLDKKSAPA
                     VVRVTHLQK"
BASE COUNT          519 a          434 c          363 g          514 t
ORIGIN      
        1 aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat
       61 ggccatactg ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg
      121 ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac agttgtgtac
      181 aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt
      241 gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg
      301 atcgttgaca cagtagcatc agttttctca atgaatgtta aacggagctt aaactcggtt
      361 aatcacattt tgttcgtcaa taaacatgca gcgatttctt ccggtttgct taccctcata
      421 cattgcccgg tctgctcttc caatgaccac atccagagtc tcttcaggaa atgcgcgact
      481 cacacctgct gtcacggtaa tgttgatatg cccttcagaa tgtgtgatgg catggttatc
      541 gactaactgg caaattctga cacctgcacg acatgcttct tcatcattag ccgctttgac
      601 aataatgata aattcttcgc ccccgtagcg ataaaccgtt tcgtaatcac gcgtccaact
      661 ggctaagtaa gttgccaggg tgcgtaatac tacatcgccg attaaatgcc cgtaggtatc
      721 attaaccaat ttaaatcggt caatatccaa caacattaaa taaagattca gaggctcagt
      781 gttgcgtaac tgatgatcaa aggattcatc aagaacccga cgccccggca atcccgtcaa
      841 aacatccata ttgctacgga tcgtcagcaa ataaattttg taatcggtta atgccgcagt
      901 aaaagaaagc accccctcct gaaaggcgtc gaaatgcgcg tcctgccagt gattttcaac
      961 aatagccagc attaattccc gaccacaatt atgcatatgt tgatgggcag aatccattag
     1021 ccgaacgtaa ggtaattcat cgttatcgag tggcctcaga tgatcaatcc accgaccaaa
     1081 ctggcacagt ccataagaat ggttatccgt tatttctggc ttactggcat ctctcgcgac
     1141 cacgctgtga aacatactca ccagccactg atagtgggca tcgatagcct tattgagatt
     1201 taacaagatg gcatcaattt ccgttgtctt cttgatcatt gccactcctt tttcacagtt
     1261 ccttgtgcgc gctattctaa cgagagaaaa gcaaaattac gtcaatattt tcatagaaat
     1321 ccgaagttat gattcatctc tgagataaca ttgtgattta aaacaaaatc agcggataaa
     1381 aaagtgttta attctgtaaa ttacctcttc attatcgcaa atgaaaggat gacaaatagc
     1441 ataacccagt acccaaatgg cccggtagtt caggccatca ggctaattta tttttatttc
     1501 tgcaaatgag tgacccgaac gacggccggc gcgcttttct tatccagact gccgctaatg
     1561 ttgatcatct ggtccggctg aacttctcgt ccatcaaaaa cggtcgcagg aataacaaca
     1621 ttaatttcac cactcttatc gcgaaaaacg taacggtcct ctcctttgtg agaaatcaaa
     1681 ttaccgcgta gtgaaaccga agcgccatcg tgcatggttt ttgcgaaatc aacagtcatt
     1741 ttttttgcat catcggttcc gcgatagcca tcttctattg catgaggcgg cggtggcgct
     1801 gcatcctgtt ttaaaccgcc ctggtcatct
//
LOCUS       DBFIFS010000395         1816 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.396, whole genome shotgun sequence.
ACCESSION   DBFIFS010000395 DBFIFS010000000
VERSION     DBFIFS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.396"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..110)
                     /locus_tag="N7A07_004900"
     CDS             complement(<1..110)
                     /locus_tag="N7A07_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479374.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYG"
     gene            complement(94..>1816)
                     /locus_tag="N7A07_004901"
     CDS             complement(94..>1816)
                     /locus_tag="N7A07_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479375.1"
                     /translation="GAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYDWNDNGQLIR
                     ISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWP
                     DNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHRLVFYTRIQY
                     GEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTGT
                     TRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRML
                     DRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHCDHRGLPLAL
                     ISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNRHRYYDPLQG
                     RYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIVANS
                     ITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIGKNHEAAGDR
                     QGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKADNPIKAKQG
                     SSDASNY"
BASE COUNT          351 a          559 c          439 g          467 t
ORIGIN      
        1 ccataaaata cgatgtcaga atttgaattg ttatcaaacc caaggtaaac aacaaataaa
       61 caaagggtaa ttaaaaaaat gacaagtttg cctctaataa tttgaagcat ctgaactccc
      121 ttgctttgct tttattggat tatctgcttt tataccatct aacccgaaga gttgcccaat
      181 gttatatttt tcaatacatg catcgctaca tttggcagaa aaatcgccac aagcaatacc
      241 gacagtgtta tttttaagat ccatgattct ttctttagct ggctgacctt gtctatcccc
      301 agccgcctca tgatttttac ctatcacgtc agctattgtt gatccaaatg tcttagtcat
      361 tcgacacatc aagtaacaat atctcattgc atcggcggcc ccattatgag ctccgccaac
      421 aatagcatcc atatcataac cgaactggta agcactgtta gtaatgctgt ttgcaacaat
      481 caatccaggt acaccagata cggcataaat tagtccacct gatgtcatat tctcaatcgc
      541 atctaacccc attgggtcta tcacttgtat gggattcaac ggatactgat acatattcca
      601 tccacctctc aacccaatcg ggtccggggt gatataccgc ccctgcaacg gatcgtagta
      661 ccggtgacgg ttatagtaca gccccgactc ctcatcatgc tgctggcccg gcagacggta
      721 cggctggtgc aggtgatgcg ggttctcctc attaagctgg ttgccccatt catcatattc
      781 cgcgctccac gccgtattgc cgtcttcgct gataagcgcc agcggcagtc cccggtggtc
      841 acagtggtac agatgcagcg tgcgcgccgg cgtgtattcc ggctccacct gtttttccag
      901 ttgctccacc gtcagtccgc actgcgcaag ccacgcccgg ctttcactgc tcacgcggtc
      961 tgcccggatt tcttcctcca gcctgtccag catcctcacc agttctgccg ggaacaccac
     1021 accgtgaccg tcctcactcc cttcctgctg gagtttctcc gccaggctgc ggcgctgcgc
     1081 tttctcccgc tcgccgttct ccgtttcgat gcggatgagc ggtgtgaagc tccccggccg
     1141 gtataccgtc tggatacgtg tggtgccggt ctgtaccgtc gtcagcctgt cgccgtccca
     1201 cccgtaccag gtcacctccg gtttacgcga cagcgacatc caccccgtca ggtcacgctc
     1261 ccgccgccac acccgtttcc ccgtccggcg gcccagcggg tcatagaggt agcggctctc
     1321 gaccagcggc tcgccgtatt gtatccgcgt gtagaacacc aggcggtgct ggttgtcgta
     1381 atggtagtgg tgggtccgct cgtcgtgcat ccggataacc ccttccggaa tgcggtccgt
     1441 cttctccgtc agcctgccgt attcatcgta gcggtagaca tagtgcgcat cttccgcgat
     1501 gcggttatcc ggccacgccg tgagcgtgct gtccggatgc agttccgggt ccggcagccg
     1561 gtttcctgcc gggtccgttg catacgggat atcgatatcc aggttcgctg ccgtggtgtg
     1621 gacgcccgtc agccttcccg tgtcgctgta acggtactcc cggctctcct gcgggccgct
     1681 gatgcggatt agctgtccgt tgtcgttcca gtcgtagtca cagtcaagct gcgggaggtt
     1741 caggtgcctg ctctgtagct gccctgtgag ggtgtacgcc gtgacctgtt cgtaccccgc
     1801 tgtactgccc gccccg
//
LOCUS       DBFIFS010000396         1811 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.397, whole genome shotgun sequence.
ACCESSION   DBFIFS010000396 DBFIFS010000000
VERSION     DBFIFS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.397"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(216..1622)
                     /gene="gndA"
                     /locus_tag="N7A07_004902"
     CDS             complement(216..1622)
                     /gene="gndA"
                     /locus_tag="N7A07_004902"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU2479376.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          486 a          421 c          417 g          487 t
ORIGIN      
        1 atgatgtttt taacatcctg ataagagcta aaagttttaa cgctacgttg ttcgatggtc
       61 aacgcagcga aaaacaattc agattttctt tataagaata gctgatattt attaagttaa
      121 tattaagcag tgatatttag tctataagat aattggcaaa aaaaagcccg gtacaatata
      181 cgccgggcct caattttatt gttgattaaa tcagattaat ccagccattc ggtatggaac
      241 acaccttctt tatcaatgcg cttataagta tgcgcaccga aatagtcacg ctgtgcctgg
      301 atcaggttcg caggcagaac agcggcgcgg tagctgtcat aataggcaac cgcagcggcg
      361 aaggtcggaa ccgggatacc gttctgtacc gcataagcga cgacatcgcg cagcgcctgc
      421 tggtagtcat cggcaatttg cttgaagtaa ggagccagca gcaggttagc gatctgcgga
      481 ttttcggcat aagcatcggt gattttctgc aggaactgcg cacggatgat gcagccagca
      541 cggaaaatct tcgcgatttc gccgtagttc agatcccagt tgtactcttc agacgccgca
      601 cgcagttgag agaacccctg agcgtaagaa acgattttgc ccagatacag tgcacggcga
      661 actttttcga tgaactcagc cttgtcgcca gctggctgcg cttgtgggcc agagagaact
      721 ttagacgcag caacgcgctg atctttcaga gaagagatgt atcgtgcaaa cacagactcg
      781 gtaatcagcg acagcggttc gccgagatcc agtgcgctct ggctggtcca tttgcccgta
      841 cctttgtttg ccgcttcatc caggatcaca tcaaccaggt agttaccgtc ttcatctttt
      901 ttagtgaaga tgtctttggt aatgtcgatc aggtagctgc tcagttcacc gttattccac
      961 tcggtaaaga tctgcgccag ttcttcgttg gtgaggttca gaccaccttt aagcagagaa
     1021 taggcttcag caatcagctg catatcgccg tattcaatac cgttgtgaac catcttcaca
     1081 tagtgacctg cgccatcggc accaatatag gtaacgcatg gctcaccgtc ttcagccact
     1141 gcggcgattt tggtcaggat cggcgcaact agttcatagg cttctttctg cccaccaggc
     1201 ataatggaag gaccttttag tgcgccctcc tcaccaccgg aaacaccggt accgatgaag
     1261 ttaaagcctt ctgcagaaag ctcacggtta cgacgaatgg tgtcctggaa gaaggtatta
     1321 ccaccatcaa tgatgatgtc acctttatcg aggtatggct taagggaatc aatagcagca
     1381 tccgtgcctg cacctgcttt caccattaac aagatgcgac gaggcgtttc cagagattca
     1441 acaaattctt tcaccgtata gtaaggaacc agtttcttgc ctggattttc ggcaatcact
     1501 tcttccgtct tttcacggga acggttgaaa atagagacgg tataaccacg gctttcgatg
     1561 ttgagcgcaa ggttgcgccc catcactgcc ataccgacta cgccgatctg ttgctttgac
     1621 attgtttact cctgtcaggg gttaccgcta gatatgcggc ttgaataacg tataaaaatg
     1681 tatcagagta ttataaattt catgctgtat cagtaataac taagtaaagg atagcttaaa
     1741 aagaagccat aatttagtaa attagcgaaa ttatatatat tttaaacatt agtcccattt
     1801 ggaacgtgtt t
//
LOCUS       DBFIFS010000397         1805 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.398, whole genome shotgun sequence.
ACCESSION   DBFIFS010000397 DBFIFS010000000
VERSION     DBFIFS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.398"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..391
                     /locus_tag="N7A07_004903"
     CDS             <1..391
                     /locus_tag="N7A07_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479377.1"
                     /translation="SINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAK
                     LPQERSEKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDV
                     APDILVLPVNVYHADFKIKINNAVEKT"
     gene            398..1312
                     /locus_tag="N7A07_004904"
     CDS             398..1312
                     /locus_tag="N7A07_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2479378.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
BASE COUNT          574 a          333 c          404 g          494 t
ORIGIN      
        1 gagtattaat caaacttact tacatgataa aaatcctggt gatgttatta gcctgtatgg
       61 cactttagat aacattcctc ttgtagttaa ggaatgcacg ggtaaaaatc atcaaattaa
      121 cgttaaagcc aaattgccac aagaacgaag tgaaaaagtt gaggatatgt gtgaaagaat
      181 gagaggtggc atctctatgt ttaatcacac cacaaaaaca gctggtaata tagagcataa
      241 tttgcagcta agttttctcg tggattcacg gccgtcgatc accaataaat actcagccaa
      301 agatgtagcg ccagacatac ttgtattgcc cgtcaacgta tatcatgcgg attttaaaat
      361 taaaatcaat aatgcagtag agaagactta gtcaattgtg ttatgtgctg tgcaaaaaaa
      421 tctccatgca gcaaggaagg ttcttagcgg gataataggt aatgtaccaa aaaataataa
      481 accaaaaaca atcatttttg atttacgttt tcttagtgca attaagcaga aagttttttt
      541 gggcagtgaa caaccgttgt ttgtaaagca taccgcaatg atgagggagg catgtaagga
      601 attaccgcag ctagtggagt atgttccttt agcctgtgaa gcgcatagcc atcgcagcgt
      661 cgcccttgcc aaaaccgtgg ctgcacccat catggcgaca ggtgtgggcg cggctttaat
      721 gtatgcaaca tcatcaggac cgtattatgc gcaaagtctt tctggttcgc cggatatcga
      781 gatgataaaa aaacgcatgg ctgaattgtt taaccagtcg gatacttctg tgcgcaacaa
      841 atatcgcccg gcttttgata aatggaataa actggtagat aaattaaatc acgaaagaaa
      901 tacagttccg tttgcgtgtt tgaccaccat tatagcgaca gcgataaatg aaactcctga
      961 gggggatacg aatgttgccg ttgtgatggg gtgtaaatct gcgaaagatc gcacgatatc
     1021 cattgtcctt ggcaactcga tgttgcaaac actctttgaa aaacgcctcg ccgatgatgg
     1081 agaaatagaa aacctttttg atcaacaggg atattttagt tgcgattcat taacagccga
     1141 agaagtaatg atgttaaaag acctttttga tattcgagtt cttcatctct ctaacaaatt
     1201 taatgttggg ttgcaaggta atatcaacac ggatgtttta caagattcgt tttttaaaaa
     1261 tgtcgatttc attcatgaat ctaactcgta taccgtaggg atgggagtct gaagagtaaa
     1321 actcattagg attatcagaa ttcggccaat gcattgcaaa aatgaaatac aaagagagat
     1381 gtgtgcaggc gcttagccgc gaggggcatg gcgaagccat acagaaagca ccggcattca
     1441 gaactcctgt gaaagcggca tagtgccgct gctcaggagt tttgttatga cccgtatcgt
     1501 gctgaacgtt gccaccctat gatgcgatta aaaaaactca ccgtcgagcc gatgattgac
     1561 gtcaatgcta tcggatgatg ccgggggtgt tccggaagga attccttgtt tcccgaactg
     1621 ttaccaacaa aattataacg atgagcaagt aaagaccggt tggtggcagt actgaccggt
     1681 ctgtgatgga agattaattt caccttaaat cctggtgctg ttaggatgct gacgtttttc
     1741 gggtacacca ttcagattat gcaaataaag attcaatgaa aaaataaata tccctgaata
     1801 taata
//
LOCUS       DBFIFS010000398         1772 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.399, whole genome shotgun sequence.
ACCESSION   DBFIFS010000398 DBFIFS010000000
VERSION     DBFIFS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.399"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            119..622
                     /locus_tag="N7A07_004905"
     CDS             119..622
                     /locus_tag="N7A07_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU2479379.1"
                     /translation="MATAGMLLKLNSQLNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(702..953)
                     /gene="yecJ"
                     /locus_tag="N7A07_004906"
     CDS             complement(702..953)
                     /gene="yecJ"
                     /locus_tag="N7A07_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU2479380.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1068..1154)
                     /gene="azuC"
                     /locus_tag="N7A07_004907"
     CDS             complement(1068..1154)
                     /gene="azuC"
                     /locus_tag="N7A07_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU2479381.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1416..1739
                     /gene="yecR"
                     /locus_tag="N7A07_004908"
     CDS             1416..1739
                     /gene="yecR"
                     /locus_tag="N7A07_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU2479382.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          460 a          423 c          391 g          498 t
ORIGIN      
        1 cagaattgac tccacgatca catttcggac cggcagaaag gaattattct gcaaacagta
       61 attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga tattaggtat
      121 ggcaaccgct ggaatgcttc tcaaactcaa ctctcaattg aaccgcgagt tttacgcatc
      181 caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg gcaccgccac
      241 tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt ttaactttat
      301 gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttcccggtg aaaaactgaa
      361 ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt ctagtacgtt
      421 ggcacagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca atttcctgcg
      481 cgatctggaa aaagaacagc agcatgatgg cctgttgctg caaaccattc ttgatgaagt
      541 gcgcagtgcg aaacttgcgg gtatgtgccc agtgcagacc gaccaacatg ttctgaatgt
      601 cgtgtcgcat cagctgcatt gatcatacat cgacgctaat gcattgcacc gatgaagttt
      661 ttgagaaacc gctgcctcat cttttttgaa gcagcggttt tttaatggga ttcgccttgt
      721 ggggtaaact tgagctcaat cagcgcgatg gctttttgga ttgcccgcat ggtgaccggg
      781 tctgcggcag cgggatgatg agtaaagtcg atattcttca gctgactgga cattttttca
      841 cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg
      901 caagcaacca catcagaaac cagttcctga tcggcattca gcgtctggga catcgtaaac
      961 tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcaac gatagtcaca
     1021 tccaccctca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata
     1081 ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt
     1141 ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtgttatt gtctttgcgc
     1201 ttataatatg acaaccatca caaaaatcaa tctttatgtg atacaaatca cataaatacc
     1261 cctttaatgt tataaaaatg ataatcaaag aacagccccc tatttctgac acctacagat
     1321 ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt
     1381 aaaatagtgc tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt
     1441 cattaataac gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg
     1501 cggcaacagg tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg
     1561 atcgttatgt cagtcgcggg atctccgatc gcgcatgcca acaggaaggc tatacccacg
     1621 cgatcccctt tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga
     1681 atacggaatt cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa
     1741 tattttaatc agcgagggga tcttcgctga tt
//
LOCUS       DBFIFS010000399         1753 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.401, whole genome shotgun sequence.
ACCESSION   DBFIFS010000399 DBFIFS010000000
VERSION     DBFIFS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.401"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..231
                     /locus_tag="N7A07_004909"
     CDS             <1..231
                     /locus_tag="N7A07_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479383.1"
                     /translation="NGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTLDFKRQLQFI
                     ILGDDKIRIWVPEGKLYFALMNVLQMFPMKNG"
     gene            356..733
                     /locus_tag="N7A07_004910"
     CDS             356..733
                     /locus_tag="N7A07_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479384.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            849..1649
                     /gene="ydcF"
                     /locus_tag="N7A07_004911"
     CDS             849..1649
                     /gene="ydcF"
                     /locus_tag="N7A07_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU2479385.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT          498 a          368 c          388 g          499 t
ORIGIN      
        1 aacggtctgg aagtgatgag tatgtttggt gaacattgtc atcacatcta ccttgatgat
       61 atcaagaaaa aaatacaaac tgaattacct gaaaataaaa ctttagattt caaacgtcaa
      121 ttgcaattta taatattagg ggatgataag attagaatat gggtgcctga ggggaaattg
      181 tatttcgcat taatgaatgt attgcagatg tttcccatga aaaatggtta ataactggac
      241 aagataattt taaaattcag caacggttat cgattataca tggtacttaa acagactcac
      301 accatttttg tagaaggtta aattgagtac agtctcatat attaagggtg ttaacatgga
      361 tgaacgtttt ataaatatca cgccgacttc gttgtctttt aaacacgccg atctgtcagg
      421 tttaagctat aagaatgcca ggcttgaact ggccctgata tgcccggcga tcaagtcaga
      481 agttaagata tcatttgact ggatacactc atttcgtgtt actgacgaag gtgatttgtt
      541 aggtatgtca ggaaagcagg gaattaacca aacggggatc tatagagtag ccaattccac
      601 ttatcttaca tggtttacta tgcaaagttg tgaaattcat aaaaataaaa tgatcgaaca
      661 ttatgttatt gccacgtcaa atgatgttat tgatgtttta tcaacagttt ctccgcaaat
      721 agtgtatgac taaaaccagt gtctggttcg cgatggcgag atagaaaaag cattatcttc
      781 ctttcagagt gctccgcttc tcactattat ctcacgcagt attcttaagg gaacgataag
      841 gaggaaccat gaacattacc ccgtttccga cgctttcgac ggcaactata gatgccataa
      901 atgttatcgg acagtggctg gcgcaggatg atttctccgg tgaggtgccg tatcaggctg
      961 attgcgtcat acttgcagga aatgcggtta tgccgactat cgatgcggca tgtaagattg
     1021 ctcgcgatca gcaaattcct ttactgatta gtggtggtat cggtcactcg accacttttt
     1081 tgtatagcgc catcgcacag catccgcact acaacactat ccgcaccact ggcagagcag
     1141 aagcgaccat cctggcggat atcgctcatc agttctggca tattccgcat gaaaaaatct
     1201 ggattgaaga ccagtcaaca aactgcggtg aaaacgcacg ctttagcatc gcgctattga
     1261 atcaggccgt agaacgagtt cacacagcta tcgttgtaca ggaccccacc atgcagcggc
     1321 gcacgatggc gacgttccgc cgtatgaccg gggacaatcc cgatgtacca cgctggttaa
     1381 gttatcccgg attcgttcct cagttaggaa ataacgcaga cagtgtaatc tttattaatc
     1441 agttacaagg attatggccg gttgagcgtt atctctcact actcactggc gagctgccgc
     1501 gtttacgcga tgatagcgat ggctacggtc cccgcgggcg agattttatc gttcacgttg
     1561 attttccggc agaagtcatc catgcatggc aaacgctgaa acacgatgcg gtgctcatcg
     1621 aggcgatgga aagtcgctcg ttacgttaaa aattgcccgt ttgtgaacca cttgtttgca
     1681 aacgggcata actcctgcct tttatttctg cctcttattc cttttacact tgtttttatg
     1741 aagcccttca cag
//
LOCUS       DBFIFS010000400         1718 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.402, whole genome shotgun sequence.
ACCESSION   DBFIFS010000400 DBFIFS010000000
VERSION     DBFIFS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.402"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            38..1714
                     /gene="tir"
                     /locus_tag="N7A07_004912"
     CDS             38..1714
                     /gene="tir"
                     /locus_tag="N7A07_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU2479386.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          479 a          375 c          453 g          411 t
ORIGIN      
        1 ttgttatcta tttacataaa ataaaaagga gatatttatg cctattggta atcttggtca
       61 taatcccaat gtgaataatt caattcctcc tgcacctcca ttaccttcac aaaccgacgg
      121 tgcagggggg cgtggtcagc tcattaactc tacggggccg ttgggatctc gtgcgctatt
      181 tacgcctgta aggaattcta tggctgattc tggcgacaat cgtgccagtg atgttcctgg
      241 acttcctgta aatccgatgc gcctggcggc gtctgagata acactgaatg atggatttga
      301 agttcttcat gatcatggtc cgctcgatac tcttaacagg cagattggct cttcggtatt
      361 tcgagttgaa actcaggaag atggtaaaca tattgctgtc ggtcagagga atggtgttga
      421 gacctctgtt gttttaagtg atcaagagta cgctcgcttg cagtccattg atcctgaagg
      481 taaagacaaa tttgtattta ctggaggccg tggtggtgct gggcatgcta tggtcaccgt
      541 tgcttcagat atcacggaag cccgccaaag gatactggag ctgttagagc ccaaagggac
      601 cggggagtcc aaaggtgctg gggagtcaaa aggcgttggg gagttgaggg agtcaaatag
      661 cggtgcggaa aacaccacag aaactcagac ctcaacctca acttccagcc ttcgttcaga
      721 tcctaaactt tggttggcgt tggggactgt tgctacaggt ctgatagggt tggcggcgac
      781 gggtattgta caggcgcttg cattgacgcc ggagccggat agcccaacca cgaccgaccc
      841 tgatgcagct gcaagtgcaa ctgaaactgc gacaagagat cagttaacga aagaagcgtt
      901 ccagaaccca gataatcaaa aagttaatat cgatgagctc ggaaatgcga ttccgtcagg
      961 ggtattgaaa gatgatgttg ttgcgaatat agaagagcag gctaaagcag caggcgaaga
     1021 ggccaaacag caagccattg aaaataatgc tcaggcgcaa aaaaaatatg atgaacaaca
     1081 agctaaacgc caggaggagc tgaaagtttc atcgggggct ggctacggtc ttagtggcgc
     1141 attgattctt ggtgggggaa ttggtgttgc cgtcaccgct gcgcttcatc gaaaaaatca
     1201 gccggtagaa caaacaacaa caacaactac tacaactaca actacaagcg cacgtacggt
     1261 agagaataag cctgcaaata atacacctgc acagggcaat gtagataccc ctgggtcaga
     1321 agataccatg gagagcagac gtagctcgat ggctagcacc tcgtcgactt tctttgacac
     1381 ttccagcata gggaccgtgc agaatccgta tgctgatgtt aaaacatcgc tgcatgattc
     1441 gcaggtgccg acttctaatt ctaatacgtc tgttcagaat atggggaata cagattctgt
     1501 tgtatatagc accattcaac atcctccccg ggatactact gataacggcg cacggttatt
     1561 aggaaatcca agtgcgggga ttcaaagcac ttatgcgcgt ctggcgctaa gtggtggatt
     1621 acgccatgac atgggaggat taacgggggg gagtaatagc gctgtgaata cttcgaataa
     1681 cccaccagcg ccgggatccc atcgtttcgt ctaaatat
//
LOCUS       DBFIFS010000401         1715 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.403, whole genome shotgun sequence.
ACCESSION   DBFIFS010000401 DBFIFS010000000
VERSION     DBFIFS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.403"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            132..386
                     /gene="bssS"
                     /locus_tag="N7A07_004913"
     CDS             132..386
                     /gene="bssS"
                     /locus_tag="N7A07_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU2479387.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            501..1619
                     /gene="solA"
                     /locus_tag="N7A07_004914"
     CDS             501..1619
                     /gene="solA"
                     /locus_tag="N7A07_004914"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU2479388.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            1667..>1715
                     /locus_tag="N7A07_004915"
     CDS             1667..>1715
                     /locus_tag="N7A07_004915"
                     /inference="COORDINATES: protein motif:HMM:NF022421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 family protein"
                     /protein_id="HGU2479389.1"
                     /translation="MRRLLHYLINNIREHL"
BASE COUNT          445 a          423 c          418 g          429 t
ORIGIN      
        1 agaaatttat ctgttccccc ctttaaaaat tcggtgatta cccttattta ttggtatact
       61 gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt
      121 agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg
      181 acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa
      241 ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg
      301 ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc
      361 cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac
      421 cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc
      481 atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag
      541 gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgcttatg accgacgccc
      601 atatgccacc acatcaacac ggcagccacc acggcgatac gcgattaatt cgacatgctt
      661 atggtgaagg cgaaaagtat gtcccgctgg tcctccgcgc acaaacgctg tgggatgacc
      721 tctcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt aaccttggcc
      781 cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg
      841 ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca
      901 actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa
      961 cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg
     1021 caattcgtca tgacgatgat ggcgtaacta ttgaaacggc tgacggtgag tatcaggcga
     1081 aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc
     1141 agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata
     1201 aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag
     1261 aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg
     1321 aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca
     1381 atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc
     1441 ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg ctcattaccg
     1501 gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg
     1561 cgcaagacaa aaaaagcgat tttgatttga cgccattcag cctttcccgc ttccaataat
     1621 catcattacg gcctagagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt
     1681 gcactatctc atcaataata ttcgcgagca tctga
//
LOCUS       DBFIFS010000402         1696 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.404, whole genome shotgun sequence.
ACCESSION   DBFIFS010000402 DBFIFS010000000
VERSION     DBFIFS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.404"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..593)
                     /gene="nleH1-2"
                     /locus_tag="N7A07_004916"
     CDS             complement(<1..593)
                     /gene="nleH1-2"
                     /locus_tag="N7A07_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HGU2479390.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAE"
     gene            complement(700..771)
                     /locus_tag="N7A07_004917"
                     /pseudo
     CDS             complement(700..771)
                     /locus_tag="N7A07_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            765..1696
                     /locus_tag="N7A07_004918"
                     /pseudo
     CDS             765..1696
                     /locus_tag="N7A07_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          506 a          419 c          357 g          414 t
ORIGIN      
        1 ttctctgcac tcccggaacc ataatactga ttgaaacaac gaacttcgct tgtcacctct
       61 tcatggcttt gagatatagt aaacatcttc aacacttttg ttgtatcttc catatcttca
      121 tacacgacag cattaccacc tttaccaata acattacctg gcacgggtga tttattgttg
      181 ctaaccctcg gcaactctga ttgagtataa tccgctggag gtaactccgg ctgcgcataa
      241 tccactggag gtaaatcagg tctgttcgaa tgaacagagc ctctttctat agtcgtgctc
      301 actggcgatg tattcagcat agcctcaatt tttctgctaa tctctccctt aggccatccc
      361 aacctgtgca acagattagt aaaacaacca ctatgacttt ctcttgtaac gcaaaactta
      421 ttatcagtga ccacaacacg atatgttctg ttgccaaccg ttacttgcgt cccgctatca
      481 gagtgaacag cagcatccct tacagaggat aaaacacgat tatcaggcga agtcaggttt
      541 ctggttaaag aattccatga acatcccaaa tttatagaag agggcgataa catacatttc
      601 aaccttcaaa ataaacctat ctaattattc ctaacagaca tcccccatac atgacaacaa
      661 aaaccggagc cggactccgg tttttgtgaa gctatcgggt tacttcattt cgccaatatt
      721 ttcccacttc ccgtcagcac gcaggatttg cagcggtctt actacacact gacccgagga
      781 cgaaaaaaga gttcgggctg ggaagagaca gaagagtagc aatttcagaa gcattcacac
      841 caacattgag cttttcagcg aaagtgggca cgaatcattg ctggacaggg ttaaaggcac
      901 tgactcaatc actcttcata cgtggcttga tcgatatgaa acaatcctca gctagagggg
      961 gagcaagccg aaaacattac tcgactacgc cagcaaaatc agggcaattc gaagaaaatt
     1021 gccggacaaa ccgctcactg acatatcaac gaaagaggtg gcagcaatgc taaacaccta
     1081 cgtcgcagaa ggtaaagcgg tttccgcaag agtaatcagg tcaacccttg ttgacgtttt
     1141 tcgaggggca atagccgagg ggcatgtggc aacgaatcca gtaacaacaa cccgtgcagc
     1201 aaagtcagaa gtaaggcgct caaggctgac agctaatgag tatgtcgcta tttaccatgc
     1261 tgctgagcac ctccccatct ggctgaggct gtcaatggat ttagctgtcg ttacagggca
     1321 gagagtgggc gatttgtgca gaatgaagtg gtcagacata aacgatggtc atcttcacat
     1381 tggacagagt aaaacaggag ccaaaattgc cattccgctg gctctaacca ttgacgcact
     1441 cgacatctca ctggttgata cactacagaa atgcagggag gccagcagca gtgaaacaat
     1501 aatcgcatca acctatcacg aaccactttc tccagccaca gtatcacggt atttaacaaa
     1561 ggcgcgaaat gcatccggga tctcgtttga tggagaccca ccgacatttc atgaactacg
     1621 tagtctgtcc gcgaggctat atcggaacca gattggctac aagtttgcac aacgtcttct
     1681 tggacataaa tctgat
//
LOCUS       DBFIFS010000403         1690 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.405, whole genome shotgun sequence.
ACCESSION   DBFIFS010000403 DBFIFS010000000
VERSION     DBFIFS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.405"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..542)
                     /gene="emrY"
                     /locus_tag="N7A07_004919"
     CDS             complement(<1..542)
                     /gene="emrY"
                     /locus_tag="N7A07_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU2479391.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVP"
     gene            complement(542..>1690)
                     /gene="emrK"
                     /locus_tag="N7A07_004920"
     CDS             complement(542..>1690)
                     /gene="emrK"
                     /locus_tag="N7A07_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU2479392.1"
                     /translation="SNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAY
                     VTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQ
                     TNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKA
                     ALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRS
                     VQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGR
                     VTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATID
                     TKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT          483 a          375 c          335 g          497 t
ORIGIN      
        1 atagggacat tgattaaaaa tatccaaccc cagctaaagt tatcacaaat ataaccgccc
       61 aatatcggcc cacatatcgg agcgataatc acggtcattg accataatgc cagagcaaat
      121 gttctttttt ctggtggata attccttaat aataaactct gtgacagtgg aattaacggc
      181 cctgccatta acccctgaac gactctaaaa aatatcagca catcaagatt gatcgataaa
      241 ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa gtaaaaataa ccttaattcg
      301 cctattcttt gtgccagcct gccggtaaca gggatcgcaa tggcatttgc tacgccaaac
      361 gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca gaaagccaga tattgtcggt
      421 attgcgacgt tagaaatagt ggaatccaac atttgcataa acgtcgctaa tgacaatgca
      481 atagtgacgc accataacgt cccaccggtt aatggtgccg gagttgattt agtgattgcc
      541 attaaagttg tccattatgc gaaataatgt tgctaatttc tttttctatc ggactggtat
      601 cgataactaa agccttactg gtataagccg gcatggacgt cacggttgaa gccagctcag
      661 gcatctcagc aatgtcttcg ttcttcgtat caatagttgc agtcatcgat aaaccaatgc
      721 gcaaggggtg ttccatgagt tcttttggat caagagaaac ttcaaccggt acacgctgaa
      781 cgattttgat ccagttccct gtcgcatttt gtgcaggtaa taaggagaac gcattgccgg
      841 ttcccatatt gatccctgtc acccggccat gaaacacaac attctcacca taaagatcgc
      901 tgataatatt gaccgattga ccaatccgta catccgtgag ttgtgtttct ttaaagttag
      961 cattaaccca catttgacgt gccggtacga cagccattaa cgattgtccg ggactcactg
     1021 tttcgccgac ctgaacactt ctctgggcaa tatagccggt aaccggactc ttaatatccg
     1081 tacgtttaag cgccaaccag gcttctttgg ttgcatccgc agcttcaacg acttgtggct
     1141 gacggtttaa tggtgtgttc attactaaag ctttattcgc tttataagcc tggatagcgg
     1201 cattcaatgc cgctttgcta cttattaacg tatctttggt atgctccagc gtttcttttg
     1261 aaataacccc ctgcttcgct aaaggcacac gacggttata atcttctaaa gattgttgat
     1321 actgaatacg tgctgaagcg acttcggcac tgtattgttt atcctgtaag tacagtttat
     1381 tcgtttgccg aacaatattt gccagattat ttttagcttt attgagtgcg atagtggcat
     1441 cagttttatc cagtgaaact aaaatgtcac cctgtcgaac gtagttcgta tctttatgat
     1501 taacgacagt gacactacct gagacttgtg cagaaattgg atctgcattc cccgtgacat
     1561 aggcgtcatc tgtactaatc atgtcttcta attccattga ccaataggca taagcacctg
     1621 aaaacgcaat aaataaaact atcgccaata aagaaaagta ttttcttctg ttagaatgtt
     1681 ttttatttga
//
LOCUS       DBFIFS010000404         1690 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.406, whole genome shotgun sequence.
ACCESSION   DBFIFS010000404 DBFIFS010000000
VERSION     DBFIFS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.406"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1690
                     /locus_tag="N7A07_004921"
     CDS             <1..>1690
                     /locus_tag="N7A07_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2479393.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DBFIFS010000405         1656 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.407, whole genome shotgun sequence.
ACCESSION   DBFIFS010000405 DBFIFS010000000
VERSION     DBFIFS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.407"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1376
                     /locus_tag="N7A07_004922"
     rRNA            <1..1376
                     /locus_tag="N7A07_004922"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1445..1521
                     /locus_tag="N7A07_004923"
     tRNA            1445..1521
                     /locus_tag="N7A07_004923"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1479..1481,aa:Ile,seq:gat)
     gene            1564..1639
                     /locus_tag="N7A07_004924"
     tRNA            1564..1639
                     /locus_tag="N7A07_004924"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1597..1599,aa:Ala,seq:tgc)
BASE COUNT          414 a          388 c          503 g          351 t
ORIGIN      
        1 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
       61 gatgtgccca gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc
      121 tagctggtct gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg
      181 ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg
      241 tatgaagaag gccttcgggt tgtaaagtac tttcagcggg gaggaaggga gtaaagttaa
      301 tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt gccagcagcc
      361 gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc
      421 ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat ctgatactgg
      481 caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga
      541 tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc tcaggtgcga
      601 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcgac
      661 ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc gaccgcctgg
      721 ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga
      781 gcatgtggtt taattcgatg caacgcgaag aaccttacct ggtcttgaca tccacagaac
      841 tttccagaga tggattggtg ccttcgggaa ctgtgagaca ggtgctgcat ggctgtcgtc
      901 agctcgtgtt gtgaaatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctttgtt
      961 gccagcggtc cggccgggaa ctcaaaggag actgccagtg ataaactgga ggaaggtggg
     1021 gatgacgtca agtcatcatg gcccttacga ccagggctac acacgtgcta caatggcgca
     1081 tacaaagaga agcgacctcg cgagagcaag cggacctcat aaagtgcgtc gtagtccgga
     1141 ttggagtctg caactcgact ccatgaagtc ggaatcgcta gtaatcgtgg atcagaatgc
     1201 cacggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccatgg gagtgagttg
     1261 caaaagaagt aggtagctta accttcggga gggcgcttac cactttgtga ttcatgactg
     1321 gggtgaagtc gtaacaaggt aaccgtaggg gaacctgcgg ttggatcacc tccttacctt
     1381 aaagaactgt actttgcagt gctcacacag attgtctgat gaaaatgagc agtaaaacct
     1441 ctacaggctt gtagctcagg tggttagagc gcacccctga taagggtgag gtcggtggtt
     1501 caagtccact caggcctacc aaatttgcac ggcaaatttg aagaggtttt aactacatgt
     1561 tatggggcta tagctcagct gggagagcgc ctgctttgca cgcaggaggt ctgcggttcg
     1621 atcccgcata gctccaccat ctctgtagtg attaaa
//
LOCUS       DBFIFS010000406         1652 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.408, whole genome shotgun sequence.
ACCESSION   DBFIFS010000406 DBFIFS010000000
VERSION     DBFIFS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.408"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..564)
                     /locus_tag="N7A07_004925"
     CDS             complement(<1..564)
                     /locus_tag="N7A07_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU2479394.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRF"
     gene            complement(567..>1652)
                     /gene="gmd"
                     /locus_tag="N7A07_004926"
     CDS             complement(567..>1652)
                     /gene="gmd"
                     /locus_tag="N7A07_004926"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU2479395.1"
                     /translation="GQDGSYLAEFLLDKGYEVHGIKRRASSFNTERIDHIYQDPHGSN
                     PNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYTADVDAIGTLRL
                     LEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVAKLYAYWITVNY
                     RESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYLGNMDSLRDWGH
                     AKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSFVGKGIEEKGIV
                     DSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWRPEITLAEMISE
                     MVAKDLEAAKKHSLLKSHGFSVSLALE"
BASE COUNT          512 a          348 c          300 g          492 t
ORIGIN      
        1 aaagcggcgc aaaagcgccg gaatcacatg agaattactt ggatgaaaat tgtcatttgg
       61 accataaaga ttggttggca ttactgaacg gtaatcacgc ccaaactgac ggttataaga
      121 ttcacataat ttaatacctg caatttttgc gatagcataa ggttcatttg ttggctcaag
      181 tttcccttgt aataattcgt cttccataat cggttggtgt gctaacttag gataaataca
      241 cgacgaaccg aggaaaagca gtttatttac attatttttg tgggcagcat gaatgacatt
      301 cgcctctatc attatattct catatataaa atcggcagga taagaactgt tagctaaaat
      361 acctccgact tttgctgctg ccaaataaac ctggtcgatt ttctgtgaag aaaaaaaatc
      421 caaaacagcg ctactatcca acaagttcaa ttcatcccga gtacgtaaaa ccaactcaac
      481 atcatcacgt tgtttgaggc gtcgggtaat agctgatcca accattcctt ggtgaccagc
      541 aataaaaata cgttgcttat tcatcatcat tccagagcta agcttacaga aaaaccatgc
      601 gattttaaaa gagaatgttt tttagcggct tcaagatctt tggcaaccat ttcagaaatc
      661 atttcagcaa gagtaatttc tggtctccaa ccaagtttga gattagcttt gctcggatct
      721 ccaagcaaag tatcaacttc agctggtcgg aaataacgag gatcaacagc aacaatgaca
      781 tcacctggtt tcacacctgg agcatcctgt ccttcaaccg aatctacaat gcctttttct
      841 tcgattcctt taccaacaaa gctcatctta ataccaagtt gtgctgctgc catttcgaca
      901 aactgacgga ctgagtattg gactcctgtt gcaatcacaa aatcttcggg ttgctcctgt
      961 tgtaacatca accattgcat tctaacataa tcttttgcat gtccccaatc tcgtaacgaa
     1021 tccatattcc ctaaatacaa acatgattcc aagccttgtg caatatttgc aagtcctcga
     1081 gtaattttcc ttgttacaaa cgtttctcca cggcgtggag attcatgatt gaacaatata
     1141 ccattacatg cataaatacc atatgactct cgataattta ccgtgatcca atatgcgtaa
     1201 agttttgcaa ctgcataagg ggaacgagga taaaaagggg tggattcttt ttgagggatt
     1261 tcctgaacaa gtccatataa ttctgaggtt gaagcttgat agaaacgcgt tttgttttcc
     1321 aatcctaaaa agcgaattgc ttccagtaaa cgtaatgtac caattgcatc gacatcggct
     1381 gtatattctg gagactcaaa agaaactgct acgtgactca tagcagctaa attatatact
     1441 tcatctggct gtacctcctt tagaattcta gtgaggttag atgaatcagt cagatctcca
     1501 tagtgcaagt gaaaatttgg gttagaacca tgtggatctt gataaatatg gtctatgcgt
     1561 tctgtattaa aagatgaggc tcggcgtttg ataccatgaa cttcataccc tttatcaagc
     1621 aaaaactcag ctagataaga tccatcttgt cc
//
LOCUS       DBFIFS010000407         1642 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.409, whole genome shotgun sequence.
ACCESSION   DBFIFS010000407 DBFIFS010000000
VERSION     DBFIFS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.409"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(129..635)
                     /gene="espH"
                     /locus_tag="N7A07_004927"
     CDS             complement(129..635)
                     /gene="espH"
                     /locus_tag="N7A07_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HGU2479396.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(666..1583)
                     /gene="escQ"
                     /locus_tag="N7A07_004928"
     CDS             complement(666..1583)
                     /gene="escQ"
                     /locus_tag="N7A07_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HGU2479397.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSKGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHS
                     NALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNL
                     SVTDVLAADLLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQ
                     LIRNNTTIAYGSIVKINEDFYFTVSIVCD"
     gene            complement(1546..>1642)
                     /locus_tag="N7A07_004929"
     CDS             complement(1546..>1642)
                     /locus_tag="N7A07_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HGU2479398.1"
                     /translation="VFLYHKKDYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          527 a          345 c          274 g          496 t
ORIGIN      
        1 tggccgctaa cgtctctgaa gattcagcct gtattatcta aaagcaatgt aatatcagtt
       61 aaaaagcgga aaaactccag aattataact attctggagt taagtcagga agaaaactca
      121 aatgctaatc ataatacgct ataagaggaa gctcttgttg ctatagccct attatcaaga
      181 cgttcggtag actgtacacg acgtaacaaa gaagataatc ctctttcagc taagatcccc
      241 tccaggggag tacgaggaaa ccaggttgaa gaaagtggag ttatatgtct ctcatttaat
      301 tttctttcca aagccttagt cacaccgcca gaaaacacgc taaatagcgt tgagaaacat
      361 ccattttccg gtgttattct ttgtactttg tacacataat catcttttgg gccatcttta
      421 ataaagtgaa cagcatagtc tttgcctcta aaagagacaa tggtggaatc ccctatttct
      481 ggatataccc gatgatggcg ttgttgcatt cgggctttaa gctgaatttc atctacgata
      541 tcttcattgg gtatatgaag ccttctgttg aacctgttcc atgacgcatg ttcacttgtc
      601 agaaatgtct tgaataccgc tcctgataac gacatgatac atctccctat ataacgtatt
      661 cctgattaat cacatactat gctaacagta aaataaaaat cttcgttgat ttttacaata
      721 ctcccgtatg ctatcgtcgt attattgcgt attaactgac aaccaccaaa aagatggtat
      781 ccatctgaat taataatgtc atctttttta acattacgca acatgtgtat atcaatatgg
      841 actgtaccga tcgcacaata aatgcgattc atgacagtct cattgctaag agcagataac
      901 agatccgcag ccagaacatc cgtgacagat aaattattat cactcagatt tactgttgct
      961 aaaggggtat taataacaat ccagcacctt ctctcttgac tatcatcaac atgatagagt
     1021 ctcagactat caccaactct caactctgta actggataat cagtccaccc aggtacgata
     1081 tagccatcaa aaaaaagagc attagaatgt ggaactaatt gccaattatc attaatcaga
     1141 tattgtaaac tttgtaccgt tgtttcgagt ataattacat taagtctgtt ttctccatta
     1201 ttattttcta ttaccggata aatatcttta tttagcatcg taaattgaac atcatcaact
     1261 gacacatcac cttccggtaa ggcagtctgc attacccata agatcatgag ttttatcgta
     1321 tcttccggta tcagccggct ttcgctatac gggaataact cttggcacca ctgaatgaac
     1381 tgttcttctg atatccagaa acgcccctca tagcccgagt atgtcaacgt gaaacgaata
     1441 ccatgaccgc tgacctcacg aaaatatgca tggcaagcat gacctttgga cgtaatatag
     1501 aggcgaccgg ttccgataac tggcaacaac ggcaaataaa aatcgttaat tttcatattc
     1561 aattgtgaac tcaatggctt catcaggaaa gatttctttt aacttatcga aaacatagtc
     1621 ttttttatga tataaaaaaa ca
//
LOCUS       DBFIFS010000408         1636 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.410, whole genome shotgun sequence.
ACCESSION   DBFIFS010000408 DBFIFS010000000
VERSION     DBFIFS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.410"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            266..>1636
                     /locus_tag="N7A07_004930"
     CDS             266..>1636
                     /locus_tag="N7A07_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479399.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIVTLPETGAATVNLINGSGKPVSVDITAHPA
                     PDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAI
                     VDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLT
                     SGKGTDA"
BASE COUNT          387 a          428 c          528 g          293 t
ORIGIN      
        1 agtggtggtt atcgggcggg gatgaaaata aaaaaatccc gcagagttag cggagctgcg
       61 ggagagaacg atgaagatta acgttatgga gttatttttc aggcatcaaa aaagtaatgc
      121 agcgtcatta ttgcggctac aggcaattgc cggaaatgtg aagagtttca gaaattttat
      181 tccgtcatga cacaggcacc ctccggggtg cctgttgttt tctggcataa acagattcag
      241 acatcagaca ggagaggggg acagagtggg taaagggggc ggcaaggggc acacgccggt
      301 agaggcaaag gacaatctta agtccacgca gatgatgagc gtgattgacg ccattggtga
      361 agggccgatt gaaggtccgg tgaaggggct gcagagtatt ctggtgaaca aaaccccgct
      421 gacggacacg gacggtaatc ccgtgataca cggtgtgact gcggtctggc gtgccgggga
      481 gcaggagcag acaccaccgg aaggctttga gtcctccgga gctgaaaccg cactgggcgt
      541 ggaagtgacg aaggcaaagc cggtgacgcg caccattacg tccgcgaaca ttgaccgcct
      601 gcgggtcacc ttcggggtgc agtcactggt gcagaccacc tcacagggtg accgtaaccc
      661 ggcatccgtc cgactgctga ttcagttgca gcgtaacggt aactgggtga cggaaaagga
      721 tgtcaccatt aacggcaaga ccacctcgca gttcctggcg tcggtgattc tggataatct
      781 gccgccccgg ccctttaaca tccggatggt cagggagacg gcggacagca ccacggacca
      841 gctgcagaac agaacgctgt ggtcgtcata caccgaaatc atcgatgtga aacagtgcta
      901 cccgaacacg gccattgtgg ggctgcaggt ggatgcggag cagtttggcg gtcagcagat
      961 gacggtgaac taccatatcc gcggtcgcat catccaggtg ccgtcaaact atgacccgga
     1021 aaaacgcacg tacagcggca tctgggacgg cagcctgaaa ccggcataca gcaacaaccc
     1081 tgcctggtgc ctgtgggaca tgctgaccca cccgcgctac ggaatgggaa aacgcctggg
     1141 ggcggcggat gtggacaagt gggcgctgta tgccattgtg acactgccgg agaccggtgc
     1201 cgccacggtg aacctgatta acggcagcgg taagccggtg agtgtggaca tcaccgcaca
     1261 ccccgcgccg gaccggatac aggtcagtac cctgcctgat ggtgtggaga catacggggt
     1321 gtggggactc tccctgccgt cactgcgccg tcgcctgttc cgctgtgtct ccgtccggga
     1381 aaacacggac ggcacctttg ccatcacggc ggtgcagcac gtaccggaaa aagaagccat
     1441 cgtggataac ggtgcccgct ttgagccgca gtcaggttcc ctgaacagcg tcatcccacc
     1501 ggcagtacag cacctgacgg tggaggtgag tgcagctgac ggccagtatc tggcgcaggc
     1561 taaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc tgaccagtgg
     1621 taagggaacg gatgcc
//
LOCUS       DBFIFS010000409         1636 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.411, whole genome shotgun sequence.
ACCESSION   DBFIFS010000409 DBFIFS010000000
VERSION     DBFIFS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.411"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..>1636)
                     /locus_tag="N7A07_004931"
     CDS             complement(<1..>1636)
                     /locus_tag="N7A07_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HGU2479400.1"
                     /translation="RSLSGADGRALYAYRCTDTEYESLAELLRTYAPRSYPRTIFISY
                     SDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYVHRQKLVNDGIRYWKRKIRNL
                     GQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQALRGQSSQYPAAKIAQELGDT
                     IPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSALRKLSPDWHLQLPLVLPEAN
                     AAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDARFRFPATMRTEQLTSLFECH
                     IQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLPIAMQKLSGADAMAEISLSLH
                     EGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLVGMGSVSSRLNALFISLPKNS
                     HLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGAVCTIRTQQLYDSAIEYYIKS
                     TEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWRSIRSGNNAWYSVASEMPKGQ
                     IEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQG"
BASE COUNT          468 a          385 c          326 g          457 t
ORIGIN      
        1 atgccttgct gagcactctc aggtataata ttaatatcga agtctgctgg taaaactaca
       61 actttaccgc taaataaaac ctcatcatta actattcgcc ggacttctat ctgtcctttt
      121 ggcatttctg atgcgacaga ataccaggca ttattacctg aacgaatgga acgccaaaaa
      181 agttctttct ccggtacaat gccatattgc agatcctttt tccaaccgat cttcggccag
      241 gctcgatgga caggataatc cgatttaacc agttcaactt ctgtcgattt aatataatat
      301 tcaatcgcag aatcataaag ttgctgggtg cgaattgtac aaacagcacc atcatgtaaa
      361 acaacgctaa atactccact tattttagtt aagctgcgtt cactgttttt cagcaacctt
      421 gggatatcaa actcaccttc tccactaata tctaaatggc tatttttcgg tagcgaaata
      481 aacagagcat ttagcctgga actcacagac cccataccaa caagttttag ctgcgattca
      541 ctctcattca tcgcttcaaa aacccaggga agttcttcag taagttcata accgcctttt
      601 ggaattgtgt gacctaacag aattggacct tcatgaagcg acaatgagat ttcggccatt
      661 gcatctgcac cagagagttt ttgcatcgca ataggtaata actcgacccg ccaatcttgc
      721 tgctcataac ggcttagcat tgccaacctt gccccgccat ttttccattt tcctgaaatg
      781 atgagtcggg tctgctccgg ctgaatatgg cattcaaaca aagaggttaa ctgttctgta
      841 cgcattgtgg ccgggaatcg gaaccgggca tcgcaatacc aactgtcatc aacatcgacc
      901 cagattcttt caacctgaag actacttgca ctgcgtattt cagaagattg agaaagtaat
      961 cgccttacaa tctctgcagc attcgcttcg gggagaacga gtggaagttg aagatgccag
     1021 tctggagaga gcttacgtaa tgcagaaacg ggatcactgc tatgaggagg atgctcacta
     1081 agtaatcggc agagagtctc gcagaattca ccggctattt catagaccag ttcgttttgc
     1141 attgtgacag gaatcgtatc gcctaattct tgtgcaattt tagcggcagg atattgagat
     1201 gattgcccac gaagcgcctg atatatcctt ctgaaatagg taatcaaata accgctctca
     1261 ttttcaatca tgcggatagg caaaccacct tcacaagcca gtgtatgcag ataacccgaa
     1321 gcttgtccca ggtttcttat ctttcttttc cagtagcgaa tgccatcatt aaccagcttc
     1381 tgtcgatgaa catacggaac tttccagtta atagaatcta aaattgtgtc ccatttaggg
     1441 tgtccgaccg tatgagtccg gcggataaac tctgcggcat atatgctaaa tagaacatcg
     1501 ctataggaaa tgaatatcgt tcttggataa ctacgggggg catatgtacg cagtagttct
     1561 gccaaacttt cgtactctgt atcagtacaa cggtaagcat ataacgcccg tccatcagct
     1621 ccggataatg aacggc
//
LOCUS       DBFIFS010000410         1618 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.412, whole genome shotgun sequence.
ACCESSION   DBFIFS010000410 DBFIFS010000000
VERSION     DBFIFS010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.412"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..905
                     /gene="espR4"
                     /locus_tag="N7A07_004932"
     CDS             <1..905
                     /gene="espR4"
                     /locus_tag="N7A07_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HGU2479401.1"
                     /translation="RWGNYTLLFENYEAYDKTGSITIEKNQGEGTLPIRHKLEFISTN
                     IAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKENVDRALVRMFKCVKLHSNELD
                     LSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKELELDFNNLTEFPQVPDGITLI
                     SVNNNLISHIDSFPPKAKKIFISHNKLSETPAIPDTAKVFDCGYNKIQEIRYFPKNLK
                     EARIGYNNIEVVPAIPGNLKILFMECNPIKEAFLMPWTLTGICYEISQRKYIVTNPAD
                     YDKYSDMVKKHVIDGEELIIKYFM"
     gene            complement(1043..1372)
                     /locus_tag="N7A07_004933"
                     /pseudo
     CDS             complement(1043..1372)
                     /locus_tag="N7A07_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1330..1540)
                     /locus_tag="N7A07_004934"
                     /pseudo
     CDS             complement(1330..1540)
                     /locus_tag="N7A07_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          495 a          316 c          341 g          466 t
ORIGIN      
        1 tcaggtgggg taattatact ctattgtttg aaaattatga ggcttatgat aagacggggt
       61 ctatcacgat agaaaaaaat cagggggagg ggactttacc cattcggcat aaattagagt
      121 ttatatcgac taatattgca gagttgctgg acaagttaac gaaaattaca gatgccagac
      181 tttgcaaagg cttcagtgac tgggctagtt cagtcaaaga aggcggatcg aatgacctca
      241 aagaaaatgt agatagagcg ttggtgagaa tgtttaaatg tgttaagctt cacagtaatg
      301 aacttgactt atcataccta tttttgggtt ctgtgccgcc tcttccagat tggattgaaa
      361 tgcttagcct tgtttataat gaacttgatt caatacaggt tcccgaatca tgcaaagaat
      421 tagaactcga tttcaataac cttacagaat ttccacaagt acctgatgga attaccctga
      481 tctccgtaaa taataacctg atatcgcata ttgactcatt tccgccaaag gctaagaaaa
      541 tttttattag tcacaataag ctatcggaaa caccagcaat accagacacc gctaaggttt
      601 ttgattgtgg ctataataag atacaagaaa ttcgatactt ccccaaaaat ctgaaagaag
      661 cgcgtattgg atataataat attgaggttg ttcctgcgat acctggcaat ttaaaaatac
      721 tttttatgga atgtaatcct attaaagagg catttttaat gccatggact ctgacaggga
      781 tctgctatga aatatcgcag cgaaaatata ttgttacgaa tccagccgat tatgataaat
      841 attccgatat ggttaaaaag catgtaatag atggtgagga actcataatt aaatatttta
      901 tgtaattaat taggcgtaat tgtgtcactg gaatagaggt ttcttgtgac gcatgtggaa
      961 ggagcgtcta tggacaaggc gtttcctgct ggtgataaat aaaggcgcag cacgtcgaat
     1021 gcaagaatgc gctgcgtctg gctcatggca attcattatc ttcgcatagc gtcaatgaca
     1081 aaaatcgact gtgtggatca aattgataat cagcaaacgg ttcgcaatca acgaaaccat
     1141 gcttcaaata aagttgatgg caggccgtaa atccagcctg tgtacccgtt tctaagctca
     1201 ggcgatgaag gcatctgtca tgggcgacct gcaaaatgtg gcgtaaaatc agactggcga
     1261 caccccgacg taaataattt ggcgcggtac gcattgattt cagttcgcca tgtttatcat
     1321 ccagcaactt tagcgcaccc attcctgcga gttgttcgcc ttcccatact gacctgttga
     1381 cctggcttgt ttaatgacat ccgatctcac cgcgtggggt tatggatggg gcaaactcac
     1441 tcaatttctg gtttaaaatg gaaacctgat tctgaaagtt ttctgccacc cacgatccgt
     1501 atacgcgata aaccctctga tcatcagaat gtctcatttg acacatcacc acgtcggatg
     1561 ttgttacgac cacagacgca acagtaacct ctggataact taatcttcgt tcttattt
//
LOCUS       DBFIFS010000411         1595 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.413, whole genome shotgun sequence.
ACCESSION   DBFIFS010000411 DBFIFS010000000
VERSION     DBFIFS010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.413"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..103
                     /locus_tag="N7A07_004935"
     CDS             <1..103
                     /locus_tag="N7A07_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479402.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
     gene            100..723
                     /locus_tag="N7A07_004936"
     CDS             100..723
                     /locus_tag="N7A07_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU2479403.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            976..>1595
                     /locus_tag="N7A07_004937"
     CDS             976..>1595
                     /locus_tag="N7A07_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479404.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVL"
BASE COUNT          416 a          299 c          393 g          487 t
ORIGIN      
        1 agcagtgaaa ccactcaaat ttgccaacca gatgtatatc gatactggcg cagtgttctg
       61 cggaaacctc acattgattc aggtacaggg agaaggcgca tgagactcga aagcgtagct
      121 aaatttcatt cgccaaaaag cccgatgatg agcgactcac cacgggccac ggcttctgac
      181 tctctttccg gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc
      241 ggaatggctg tattctgtgg taagcacgaa ctcagccaga acgacaaaca aaaggctatc
      301 aactatctga tgcaatttgc acacaaggta tcggggaaat accgtggcgt ggcaaagctt
      361 gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat
      421 tgccgtagtg ccgctacgcc gggcgcaaga tgcagagatt gccacggtac aggccgtgcg
      481 gttgatatag ccaaaacaga gcagtggggg atagttgctg agaaagagtg cggaagatgt
      541 aaaggcgtcg gttattcaag aatgccagca agcgccgcat atcgcgctgt gacgatgcta
      601 atcccaaacc ttacccaacc cacctggtca cgcactgtta agccgctgta tgacgctctg
      661 gttgtgcaat gccacaagga agagtcaatc gcagacaaca ttttgaatgc gatcacacgt
      721 tagcgccatg attgccacgg atggcaacat attaacggca taatattgac tttttgaata
      781 actttgggga aacttgacac caataatggg cgttttttac atgtcattga tgagtctcaa
      841 taacctgccg ccgagtagtt tttatgctct gaattgtatt tgtgtagtaa acatgctgac
      901 tgcaatgtaa tagagttttt ttagcctgta acctcttgac ggcattgaat tgcttttgtt
      961 atgagttgta agccaatgtt atcatcttgt attggggtgg ttatgaagga tggtgcgctg
     1021 ctcaggagtt cttcactttt tattgcctac atgggatgcc ttggatgggg gagtgcttat
     1081 ttctatggat ggggtacttc tttttactac ggcttcccat ggtggattgt aggtgcaggt
     1141 gttgatgatg ttgccagaag tttatttttt gcagttatcg tcattgctat atttcttatc
     1201 ggttggggta ttggtgttgt attctttttc gcagtgaaaa gaaaacattc tatgcaagag
     1261 ctaaatgtat ttcgccttta ttttgctgtg gaattattgt ttgtgccggc aattattgag
     1321 ttttctatat tgagacagaa gattcaggta cctcttttgc tactgtcagc agcgattgcg
     1381 ctggcggtca caatttcgat aagatcttat gggcgatttt tatcggtatc atgcttctat
     1441 gataagccat ttataaaaaa acattttttt gagattgtga tgattgcttt tgtggcatat
     1501 ttctggcttt tttcatttct gacaggatat tacaaaccgc agtttaagaa agaatatgaa
     1561 atgattaatt ataatgatgg ttggtattat gttct
//
LOCUS       DBFIFS010000412         1587 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.414, whole genome shotgun sequence.
ACCESSION   DBFIFS010000412 DBFIFS010000000
VERSION     DBFIFS010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.414"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(35..>1587)
                     /locus_tag="N7A07_004938"
     CDS             complement(35..>1587)
                     /locus_tag="N7A07_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479405.1"
                     /translation="EYGYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDS
                     TLTAWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVF
                     YTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLT
                     TVQTDTTRIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPA
                     ELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHR
                     GLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRY
                     YDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSS
                     IDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSET
                     KSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACR
                     TWQECWVN"
BASE COUNT          316 a          506 c          370 g          395 t
ORIGIN      
        1 actaaaaata tatatttttt catatttcct cctgttaatt gacccagcat tcttgccacg
       61 ttctacaagc cagagtcata actgaagcgt caacacctgc accgatacca acagattctg
      121 tcgatgattc tggattatca acatccacta cataagtata cgcaaaatgc cccccaactc
      181 cgccgccaac cgttacacct actgaattgc tgactcccga tgtaggtaaa gacttggtct
      241 cagaatatgt gatagccgca tttatcccac caccaattcc tgcatgtccg catgctgtag
      301 cataaataca aatatcagat aacatttcac ctttcttttt agcaaacgca acgccaagtc
      361 ctgcatgacc acctaatgat attagatctc cactgccagt aatatcaatt gatgatgttc
      421 catatgaatc atcatgccaa acatcatata ataattgata taaattatac aaacccaaag
      481 gatccatcct tattatcgga ttcagtgggt aattatacag attccatccc cccgccagcc
      541 caatggggtc ttgtgtaata tacctcccct gtagcgggtc atagtaccgg ttccggttat
      601 agtacagccc tgattcctca tcatgctgct gccccggcag acggtatggc tggtgcaggt
      661 aatacgggtt ctcttcatta agctggttgc cccattcatc atactccccg cgccacgccg
      721 tattgccgtc ttcgctgatg agcgccagcg gcaggccccg gtggtcgcag tggtaaaaat
      781 gaacttttcg cgccggtgtg tattccggct ccacctgtct ggccagttgc tccaccgtca
      841 gcccgcactg cgcaagccac gcccggcttt cactgctcac gcggtctgcc cggatttctt
      901 cctccagcct gtccagcagc cgcaccagtt cagccgggaa caccacgccg tggccgttct
      961 cactcccttc ctgctggagc gtctctgcca ggctgcgccg ctgcgctttt tcccgctcgc
     1021 cgttctctgt ctcgacccgg atgagcggcg tgaagcttcc cggctcgtat accgtctgga
     1081 tacgtgtggt gtcagtctgc accgtcgtca gcctgtctcc gtcccagcca taccacgtca
     1141 cctccggttt acgcgacagc gacatccacc ccgtcaggtc acgctcccgc cgccagaccc
     1201 gttttgccat tcgccgtccc agcgggtcgt agaggtagcg gctctcgacc agtggctcgc
     1261 catgctgtat ccgcgtgtag aacaccaggc ggtgctggct gtcgtagtgg tagtggtggg
     1321 tccgctcgtc gtccgtccgt atcacacccg ccgggatgcg gtccgtcttc tccgtcagcc
     1381 tgccgtattc atcgtgtcgg tagacatagt gcgcatcctc cgcgatgcgg ttatccggcc
     1441 acgccgtgag cgtgctgtcc gggtgtagct ccgggtccgg cagccggttt cccgccgggt
     1501 cggtcgcata cgggatgcgg atatccaggt ctgatgcaag ggtgcgcacg ctctccagcc
     1561 tgcccgtggc actgtagcca tattccc
//
LOCUS       DBFIFS010000413         1566 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.415, whole genome shotgun sequence.
ACCESSION   DBFIFS010000413 DBFIFS010000000
VERSION     DBFIFS010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.415"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1566
                     /locus_tag="N7A07_004939"
     CDS             <1..>1566
                     /locus_tag="N7A07_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2479406.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPE
                     KEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVRFS
                     LRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTFRIN
                     APAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGS
                     QWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLA
                     QGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLENQSAIIQQIQRVQKDTNDD
                     LAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTTPLF
                     VAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSGTLN
                     NVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDR
                     QIVIPAVAFSGAK"
BASE COUNT          376 a          404 c          477 g          309 t
ORIGIN      
        1 tgatggtgtg gagacatacg gtgtatgggg actatccctg ccgtcactgc gccgtcgcct
       61 gttccgctgt gtctccgtcc gggaaaacac ggacggcacc tttgccatca cggcggtgca
      121 gcacgtaccg gaaaaagaag ccatcgtgga taacggtgcc cgctttgagc cgcagtcagg
      181 caccctgaac agcgtcatcc ctccggcagt gcagcacctg acggtggagg tgagcgcagc
      241 tgacggccag tatctggcgc aggtgaaatg ggacacgccg cgggtggtga agggcgtgcg
      301 cttcagtctg cgcctgacca gcggaagcgg agaaggcagc cgtctggtga ccaccgccat
      361 cactgcggat acagagcatc gttccagtgg tctgccgccg ggggaataca ccctgacggt
      421 cagggcgatt aacagttatg gccagcaggg ggaaccggcc accaccacgt tcaggattaa
      481 tgcacctgcg gtacccgcca cgattgagct gacaccgggc tattttcaga taacagcggt
      541 cccgcgtctt gcggtgtatg acccgacggt acagtttgag ttctggtttt cggagacaaa
      601 aatcgcagat acatctcagg tggaaacctc tgcccgttat ctggggaccg gcagtcagtg
      661 gactgtccag ggaagccgga ttaagccggg gacggatttc tggttttacg tgcgcagcgt
      721 caacctggtg gggaaatctg cgtttgtgga agtcagcggg cagcccagca atgatggtga
      781 agggtatctg gaatttttcc gggaaaaaat aggaaaactg catctggctc aggggctatg
      841 ggagctgata gacaacagcc agcttgcgga tgagatggcg gagatgaaga ccaccatcac
      901 ggaaacccgc aatgaaatca cacagacggt cagtaaaacg ctggagaacc agagcgccat
      961 catacagcag atacagcgcg tgcagaagga cacaaatgat gaccttgctg cactttacat
     1021 gctgaaggta cagaaaacaa aaaatggcat accctatgtt gccggtattg gagcggggat
     1081 tgaggatact gatggccagc ccctgagcaa catactgctg ctggctgacc gtattgcgat
     1141 gattaacccg gaggacggca acaccacgcc gttatttgtg gcgcagggga atcagttgtt
     1201 catgaacgat gtgttcctga aacgactgtt tgcggtgagt atcacgtcat ccgccaatcc
     1261 cccgacgttt tccctgacgc cggagggcag gctgaccgca agaaatgctg atatcagcgg
     1321 taacgtgaat gcgaattccg ggacgctcaa caacgtcacg attaacgaga actgtcgggt
     1381 tctgggaaaa ctgtccgcga accagattga aggcgatctc gttaaaacag tgggcaaagc
     1441 tttcccccgg gattcccgtg caccggagcg gtggccatca gggaccatta ccgtcagggt
     1501 ttatgacgat cagccgtttg accggcagat tgttattccg gcggtggcat tcagcggcgc
     1561 taaaca
//
LOCUS       DBFIFS010000414         1560 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.416, whole genome shotgun sequence.
ACCESSION   DBFIFS010000414 DBFIFS010000000
VERSION     DBFIFS010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.416"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..>1560)
                     /locus_tag="N7A07_004940"
     CDS             complement(<1..>1560)
                     /locus_tag="N7A07_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2479407.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPE
                     KEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFM
                     LRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRI
                     AAPAAPSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTG
                     LYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHL
                     AQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNN
                     NLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMINPANGNTKPM
                     FVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTSDGKLTAKNADISGSVNANSGTL
                     NNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFD
                     RQIVIPAVAFSG"
BASE COUNT          319 a          474 c          399 g          368 t
ORIGIN      
        1 gccgctgaat gccaccgccg gaataacaat ctgccggtca aacggctgat cgtcataaac
       61 cctgacggta atggtccctg atggccaccg ttccggtgca cgggagtccc gggggaaagc
      121 tttgcccact gttttaacga gatcgccttc aatctggttc gcggacagtt ttcccagaac
      181 ccgacagttc tcgttaatcg tgacgttgtt gagcgtcccg gagttcgcat tcacactgcc
      241 actgatatcc gcatttttag cggtcagctt tccgtctgat gtcagggaaa atgccggagg
      301 attgccgcca ctggtaatgg tgggagccgt cagatatttc aggaacactt cattcatgaa
      361 tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg ccattcgcag gattaatcat
      421 cgcaatcctg tctgccgcca gcagcacctg actctgcatg ccgtcagagg tgttctcaat
      481 accggcaccg atacccgcaa tataaaggcg tccgtcctgc atctgctgca gcttcactgc
      541 ccacatgctg ttcaggttat tatttgtatc aacctgaacc ttctgtatct gctggatcgc
      601 tgcactctgg tcttccagtt tcttattgac ggtctgcgtg atttcattac tgacatccgt
      661 gatggacgtt ctgatttccg ccaggtcagg cgcaagctga ccgttatcaa tctgagtcca
      721 caactcctga gccagatggg ttttccctat ctctcctttg aaaaaatcca gatagccgga
      781 tgcatcatca ctcggctggc caacagcctc cacaaatgcc gatttgccaa cggtgttcac
      841 actgcggata taaaagtaat aatcatggcc cggtttgata ttgatactgg cggctatcca
      901 gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc tgcctgatat cggtaatccg
      961 cgtttccgaa aaccagaact caaactgtac cgtcgggtca tacaccgcaa gacgcgggac
     1021 cgctgttatc tgaaaatagc ccggtgtcag ctcaatctgt gacggcgctg ccggtgcggc
     1081 aatccggaac gataccgacg ccggatcgcc ctgctgcccc cacgcattta ccgcccggac
     1141 tgtcagcctg tagttccccg gcgccagttg cgtgaagcgg tatgtggttt ccgtcgtccg
     1201 ggccgtgctg accagccgct cactgccgtc atccgcggcc acggtcaggc gaagcataaa
     1261 gctcacgccc ttcaccacct tcggcgtgtc ccatttcgcc tgcgccagat actgaccgtc
     1321 agctgcgctc acctccaccg tcaggtgctg cactgccgga gggatgacgc tgttcagggt
     1381 gcctgactgc ggctcaaagc gggcaccgtt atccacgatg gcttcttttt ccggtacgtg
     1441 ctgcaccgcc gtgatggcaa aggtgccgtc cgtgttttcc cggacggaga cacagcggaa
     1501 caggcgacgg cgcagtgacg gcagggagag cccccacaca ccgtatgtct ccacgccatc
//
LOCUS       DBFIFS010000415         1525 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.417, whole genome shotgun sequence.
ACCESSION   DBFIFS010000415 DBFIFS010000000
VERSION     DBFIFS010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.417"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            97..1326
                     /locus_tag="N7A07_004941"
     CDS             97..1326
                     /locus_tag="N7A07_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2479408.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          379 a          329 c          316 g          501 t
ORIGIN      
        1 tagaggggta gatagggcgt taatctccca tacttaacct ggtttatggt aaattgccct
       61 ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg gtctaaagaa
      121 gaaactctgt ggagtttcgc gctctacggc acagccgttg gtgcaggcac gctcttcctt
      181 cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct ggtcgcctgg
      241 cctttaacat actggccaca taaagcttta tgccagttca tcctctcatc gaaaacatca
      301 gcaggtgaag ggataacggg cgcggtaaca cactactatg gcaagaagat tggtaatctg
      361 attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt
      421 accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg catccgtatg
      481 ttggtgagtc tgggtgttgt attaattctg aatctcattt ttctgatggg acgtcatgcc
      541 actattcggg taatgggatt tttggtattc ccattgattg cctatttctt atttctttcc
      601 atttaccttg taggtagttg gcaacctgat ctattaacaa cccaggtaga gttcaatcag
      661 aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt tagccatacg
      721 cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca cgctatggat
      781 aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt actgttcttt
      841 gtctttagct gcctgctttc tattccacct tcgtatattg aagcggctaa agaagaaggg
      901 gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggtt gtcaatttcc
      961 gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt
     1021 attgaaggtg ccacggaggt cgtcaaaaca acactacagc aggttggtgt aaagaaaagt
     1081 cgtgcattta accgcgcact atcaattatg ttggtatcgc tgattacctt cattgtttgt
     1141 tgcattaacc cgaacgcgat ttcgatgatt tatgcgatca gcggcccgct cattgccatg
     1201 atacttttca tcatgcctac gctatcaaca tatctcatcc cggcgcttaa accctggcgc
     1261 tccatcggaa atctgattac gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt
     1321 agctaatatc cattgtgaat ggttcaaaat tgctacaatt ttgaaccatt ccagttttca
     1381 taatgtgcat ttgttattat tccgctctat cacttctctc gtcatatcct agtattatca
     1441 gttagttaaa cacacaaaga gatctgaaca tcttaaataa gactatttaa gatgcataac
     1501 ttagattcgc aagatatctt ctggt
//
LOCUS       DBFIFS010000416         1521 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.418, whole genome shotgun sequence.
ACCESSION   DBFIFS010000416 DBFIFS010000000
VERSION     DBFIFS010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.418"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            58..321
                     /locus_tag="N7A07_004942"
     CDS             58..321
                     /locus_tag="N7A07_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU2479409.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            623..763
                     /locus_tag="N7A07_004943"
     CDS             623..763
                     /locus_tag="N7A07_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2479410.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          419 a          308 c          370 g          424 t
ORIGIN      
        1 tgaccgtctg aatatgtacc tttatgcgga attttcactt aaagggggaa aactgttgtg
       61 aaaccgaata tccatccgga ataccgtaca gtagtatttc acgataccag tattgatgag
      121 tacttcagga tcggttcgac gatcaaaact gaccgggtaa ttgaactgga tggtgtaact
      181 tacccatatg ttacaattga cgtttcttct aagtcgcatc cgttttatac aggtaaactg
      241 agaactgtcg cttcagaagg taatgttgca cgctttaccc agcgttttgg gcgttttgtt
      301 gatgcgaaaa aaggtggctg attttgccgc cagggtgcgt ttgacaattt aaaaacgcac
      361 cagacagaga gatcctgcac ctgaatatac cgttagcccg tcccaccggg gcgtgtgcag
      421 atgatctcgt caccggaaga ttgcggacct ctctgaaaac cagtacatta cgcgcgggtg
      481 cctttcggct gacggctgga aggattacct gaaggccgga tgtggaaagg ccccgagtca
      541 acttaaacgt taacccgagg ccctgaccat ctaccttcag caagtggaag gttagcgcct
      601 ctctgtaaaa ggagcaagtg ctatgccgca aaaaacgatc attgtgggca tgttatgcct
      661 gaccatgttg ctgaccgtct gggtgttaca cgcatcaccg tgtgaattca gggtaagctt
      721 catgtggtcg gaaattgcgg cgttcttgca gtgtaaaccg taagaaaccg cagcggggac
      781 aacagtcccc gctatccggt tgccaaaggt tcggtcgaaa gtcgcaccct tgcacacaaa
      841 tcacaaagaa aaaacaaaag tccgcagtgt aaaaactgtg ggctttttta ttcgcatccc
      901 gaaagtgtgg taacagcgtg ggggcaccaa aaaaagcccc ataaaatggg gctgaagatt
      961 ctgtaaggaa tttcacagca atgagattcc tgctttctcc aaggatatac gtgtcttcag
     1021 gctgaatcaa tgggggcgtg gctctgaatc cacctgcgat taaaacttgc acaatgccgg
     1081 gagttgagtt agaaaagtac agggcgcgat atccggctta ttttgttgct gtgttttgcc
     1141 tcgtgctgga tgtcgcgtcc accgtttttc tgatgattca tatgaaatgg tactttattg
     1201 atttactgcc attctgattt gcctttttat gttcattatt gcggttgtca cttccagttt
     1261 ctggtgatgt tttgataaaa gtgaaagaat aaaacgataa ttgcagaagg tattgctaaa
     1321 actatacata tcattatata aacaagatga aaatgatata taccataaga ccagaaaaga
     1381 ggttttttcc agaatgatat ggatgtgtat tttagtgtga ttgtttttgt caggtatatt
     1441 gaacaaggaa gggccaggcc actgagtatc agaaataagt caacttttat cgatgcttct
     1501 gttggtgtgt catatatcag a
//
LOCUS       DBFIFS010000417         1506 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.419, whole genome shotgun sequence.
ACCESSION   DBFIFS010000417 DBFIFS010000000
VERSION     DBFIFS010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.419"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..771)
                     /locus_tag="N7A07_004944"
     CDS             complement(<1..771)
                     /locus_tag="N7A07_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2479411.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKF"
     gene            complement(840..1265)
                     /locus_tag="N7A07_004945"
     CDS             complement(840..1265)
                     /locus_tag="N7A07_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2479412.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1262..1489)
                     /locus_tag="N7A07_004946"
     CDS             complement(1262..1489)
                     /locus_tag="N7A07_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2479413.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
BASE COUNT          338 a          446 c          332 g          390 t
ORIGIN      
        1 aaatttctgc tcccactgaa cctgcgtgaa aaccttcccc tccgccatcc agtaatcccg
       61 gaatgcggca agctcagcag gtgtaaactc aggctcaggc agagccatac cccacactgc
      121 tgcccgctgt cgaaaatccg gcgacggttg ccagactcca gtcatcggaa atttcccgat
      181 cggttcgctc aggccgtcca ggtattcagg ttcggctgtc tgcaacgacg cgtcattcaa
      241 ctcaccggtc gtagcactct cgcgcatgcg cgcgttatgt gtggggttta attctttatc
      301 tgtatctgta tctgtcgtga tttgtcgtga catatgcgtg acgcgtcgtg actcatcgtg
      361 acaatcagtg ttctgcttcc gcagtctttc ccgctcccgc tgcgctctct tgcgctctgc
      421 cggggatttt gcggtttgcg aaacattacc gttatcctcc ttcatcacct ggcgtttttc
      481 ccatccggaa ataagatcac catccagaac tcgcccctgc attgcatgca aaattgaatc
      541 aatcacgtct tccgtcacat caagcgcact tgctaaatct tccgtcgtga catcaatgtg
      601 accacgtagt gacacgccgt gacatgtcgt gacatttcgt gacgcactca ccagaaggtg
      661 gatatacacc gccatcactg ttgcgattgg ctgtcctgag accctggcaa tggttcgcca
      721 tttgggatca tttggcatgt catgccacaa tctgagccag gcattagcca tactcacctc
      781 atctgatacc gaactttacc ctcgaacatc cggaagaaat ccggcatgaa tattgttggt
      841 caatgcacga caacagcatt accaggctga ccaccactgt tagtcagggt gccccaggcg
      901 atcgccgctg cgacaaaatc atccacatct ttcaccagcc gatccctccg ttcgacgatc
      961 tcacggtaat attcagagct gtgactgcgc atacgggcca ccagcagagg cggcattgcc
     1021 ttttcgatcg ccggtaacag agcctgaatt ttttcaacag catcaggggt gtctttatcc
     1081 agccaacgga aaattttctg ggtattacga gccagggctt ccggatggct gtcgtcgtac
     1141 agttccggga acgtcatccc cagctcgaaa taagtccggg ctatttcagc tgcaggaact
     1201 ttctcaccgt ccggataggc ccaggcattg atcgccatgc ggatgtgctc atgtttgatt
     1261 ttcatgaatc cccctttcct tcgccctgag tggtatcctt ctttttgtaa agttctgggt
     1321 tcaaagataa tttccccttg gagtatgcag cagcttccgc agccctcccc ttcggaacta
     1381 tttcaccagg acgcttacgc cacatgtaaa tagcttcgcg ggttatccca taaaaatcgg
     1441 caaccctctg aacagaacca aaaaactgga caagttcatc aactcgcatt ttacctccca
     1501 aatcta
//
LOCUS       DBFIFS010000418         1506 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.420, whole genome shotgun sequence.
ACCESSION   DBFIFS010000418 DBFIFS010000000
VERSION     DBFIFS010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.420"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            150..1352
                     /locus_tag="N7A07_004947"
     CDS             150..1352
                     /locus_tag="N7A07_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2479414.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          418 a          299 c          393 g          396 t
ORIGIN      
        1 taaatctgac ttaatccagg ggtgttatat aatttcatta taaacaatgt attacacttt
       61 ttgttgtccg atattatcta atgtattctt agtgaagcga gagactaatg gcatactgga
      121 tggcatacca gagtgacggg aattagcgta tggcagtatt gacggatacg aaagcaagac
      181 atatcaaacc tgatgacaaa ccattgcccc atgggggaat taccggactg acccttcatc
      241 cttcttcagt aaaggggcgg gggaaatggg tttttcgtta tgtaagtccg gtgacacaaa
      301 aaagacgtaa tgctggattg ggaacttacc cagaggtcag tattgctgaa gctgcacgta
      361 ctgcccggat aatgcgagag caacttgctg caggtgatga tcctctggag attaaaaagg
      421 ctgaatctga gaaagtcgct atcccaacat ttgccgatgc agccaggcgt gtacatgcag
      481 aactgtctcc tggatgggaa aatccaaagc atgtaaggca gtggttatcg acgcttgaga
      541 attacgcgtt tcctcaactg ggagcaaaaa cgctggattc gattacggct gcggacgtgg
      601 cagaaacact gcgtccagtc tggttaacct tgtcagaaac ggcaagccgg gttaaacagc
      661 gcattcatgt tgttatgcag tggggatggg cgcacggttt ttgtgtagca aatcctgttg
      721 atgtggttga ccatttgctt cctcagcaga caagaggacg tgatgaacac caacccgcaa
      781 tgccctggag gcagttaccg ctttttgtgg cgaccagtgt gtataccgat gaaccttata
      841 atgttacccg cgcactgtta ttaatggtga tacttacagc aactcgctcg ggcgaagcca
      901 ggggaatgcg ctgggctgaa attgattttc ataagcgggt atggactata cctgcagaaa
      961 gaatgaaagc caggatacag catcgtgttc ctttatcccg gcaggctatt tacattctgg
     1021 aaaatatacg tggcctgcat gatgaactgg tgttcccttc acccagaaag cagcagatcc
     1081 tttccgatat ggtgttgaca agttttctgc gtaaaaagaa agccgtcagt gacattccgg
     1141 ggcgagttgc cacggcacat ggttttcgct caacattcag ggactggtgt agcgaacagg
     1201 ggtattcgcg ggatctggcg gaaagggcgc tcgctcatac gctgaaaaat aaggttgagg
     1261 cggcatatca tcgtactgat ctactggagc agcgtgtacc gatgatgcag gcatgggcgg
     1321 attatgtgat gtctcaaatt gtgaataaat aatagtgaaa atgtatattg tacgatcatg
     1381 atttgatatg tgatcgtagc agtagtaatt aagaaaccgc ttttatgtca aatgtattga
     1441 gctgataatt atcagatcgt caggaaagcg tattttgatt aacaggggat tacttaacaa
     1501 tcccct
//
LOCUS       DBFIFS010000419         1489 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.421, whole genome shotgun sequence.
ACCESSION   DBFIFS010000419 DBFIFS010000000
VERSION     DBFIFS010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.421"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(43..468)
                     /locus_tag="N7A07_004948"
     CDS             complement(43..468)
                     /locus_tag="N7A07_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2479415.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            555..770
                     /locus_tag="N7A07_004949"
                     /pseudo
     CDS             555..770
                     /locus_tag="N7A07_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(813..1152)
                     /locus_tag="N7A07_004950"
                     /pseudo
     CDS             complement(813..1152)
                     /locus_tag="N7A07_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          377 a          369 c          351 g          392 t
ORIGIN      
        1 cgtcggcaac gagccagatg cgggtacctg acgggagtga gatcatcgtc ccctcccggt
       61 cagttcacgg atcaacagcg tgagcagctc tggcgatgga ttttccagcg tcatgttacc
      121 gtgacggaac tccaccttgc aggagccggc actgacactg gtctgagtgg aagtggataa
      181 agacggcgca atggccgcca ccggttcttt ctgctcatcc ggcgttattt ctacaggtaa
      241 taattcaacg ccagtgtcag aagaggtcgt tacctgaaga cgccgtgata tacgcccttc
      301 gttctgccag agcctgagcc atttgaacag gaggttatca ttgatatcgt gttcccgggc
      361 aatacgggca acagaggctc ctggttgtga tgccagttcc accatacgaa gtttgaattc
      421 attcgaatag tttttacgag gttcttttcg ccagtcctgt aattccatac ttagatgtcc
      481 gtctatatca gatgggcgtc taagttacca attttcgtct gatggctaca tacggcggtc
      541 agtttacgct tactgacaac gcgatggcgg agagcatcaa tggtctttac aaagcggagg
      601 taatacaccg taagagctgg aaaaaccgtg cagaagtgga actggccaca ctcacgtggg
      661 tggactggta taacaatcga cgattgctgg aaaggctggg ccatactcct ccggcagaag
      721 cagaaaaagc ttattatgct tccatcggaa acgatgatct ggcagcctga gttcacagat
      781 aaaacactct ccaggaaacc cggggcggtt caaacctgat tctggaaaag gactttgtgg
      841 attgttgcta ttgccagtga gattgaatga agtcgtttta actcgctttg aatacgtctt
      901 gcccccagat ttcgctgaga acgtaactcc agaatcagag cgacttcgca agtgccggtt
      961 ttcgttgatg gtgaacgctt ggggcgacgg ctgtggcttt ccagaccagc gatgccctga
     1021 gccagatatc tgcgccacca ttttacgcaa tgtgggccga gaaatgccac agcgacggca
     1081 tacaaatccc gcatcaccgg acgtttcata gagttttacc cactgtagcc gctgctgaat
     1141 ttccctgttc ataatttggc attagttcag ctcaatcccg acttaaaaag tccctacagg
     1201 atgttccgga cctgaggcac agttcatatc cttatccata aaaaatttag ctatgtctat
     1261 agcattaatc ttgacaagac gacgttaggg cctggcgatg aatcatttcc cctgccagat
     1321 atcctttggg gtgcaccaga actaccgttt tatatctata taactgaaac gtccctcgcc
     1381 atccttcata ttttgatgta gtaacaccat tcactttcat ctaccccccg ttttattaac
     1441 atatagtaaa aatcacctcg ccgcatttta ttcttttgaa gtaaaatca
//
LOCUS       DBFIFS010000420         1469 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.422, whole genome shotgun sequence.
ACCESSION   DBFIFS010000420 DBFIFS010000000
VERSION     DBFIFS010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.422"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(1..722)
                     /gene="sctT"
                     /locus_tag="N7A07_004951"
                     /pseudo
     CDS             complement(1..722)
                     /gene="sctT"
                     /locus_tag="N7A07_004951"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(724..984)
                     /locus_tag="N7A07_004952"
     CDS             complement(724..984)
                     /locus_tag="N7A07_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU2479416.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(995..>1469)
                     /locus_tag="N7A07_004953"
     CDS             complement(995..>1469)
                     /locus_tag="N7A07_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU2479417.1"
                     /translation="MPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSYLIKYSE
                     PELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVV
                     VDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          516 a          315 c          236 g          402 t
ORIGIN      
        1 ggcatcactc gttcagccag tattgggcca aaataaataa ttaatattaa aaatgccagc
       61 gcacttttga cagtcagtga aatggcaaaa gcatttagtt gtgaagcata gcgtgccagc
      121 aatcccagga cagcctcacc acccagcata acggcaatta cggggcttgc gtaaacgata
      181 gtatgagtca ttatattatt aatatatgaa aataaaggct ctaatttagg aaaattaaaa
      241 cttcctgaag gccataaatt ataactttgc taaagagttt ccagaataaa gttcaatcca
      301 ccgttagtaa gataaaccgc tgcagagaaa aggttaaata atcttgctaa ttccgaggta
      361 tcgacacctg tagccggatc caaagtacta cttaacgttg caccgcgttg gttatcgata
      421 aagctaccta tagcaaggaa aatccaaaat ggagatgcta ataagcaagc aatgaacagc
      481 cctatgataa tttctcttgc agctaacaaa gcgatatcga tttcatttaa ggtcgataat
      541 tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg atgacggact
      601 actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg tgccaatcgg
      661 caaaaaccga gcgctgtagc agccagtaga ctatgcaact gatagagtat cacctccccc
      721 atgctatcct ctcgcaaatg ctaaattcag catttcaata ccaaaagaat ataatttttc
      781 accataccat ccgctcatta aaaaaaagca aatacttacg cacaataatt tcacgccaaa
      841 tggcaacgtc tgttcttgca actgcgttac cgtttgaaaa agccctacca ataaaccaac
      901 aaatgtcgct actgcaatag gtcctgcaga cataactaaa atcagataca gcgccctatt
      961 ccctgcaaaa acaatgtcat ccatattttt ttcctcaggg attaatggat aggtcaaaat
     1021 attgtaatat gagtcctttt gataacattg tccaaccgtc catcgcgaca aacagaatta
     1081 attttatcgg tgtagatatg gttaccggac tcatcatcat catacctaac gttaacagaa
     1141 cgctggatat aaccagatcc actacaacaa agggtaaata gatataaaaa cctataataa
     1201 atgcactttt tatctcactt aacgcatatg ccgggagtaa cgagaaaatt gaaatattat
     1261 catcatcgat tatctcttca ttgtcttcag aactattcac cttctgaatc ttttcaaaaa
     1321 agctaaccaa ctccggttca gaatatttta tcaaatatga tttataacca ctcatgcctg
     1381 tctcaacaaa attaacaacc gacgctacat tattgaatga aagattctca ttctgactgt
     1441 tataatatat ttccttccca accggcatc
//
LOCUS       DBFIFS010000421         1465 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.423, whole genome shotgun sequence.
ACCESSION   DBFIFS010000421 DBFIFS010000000
VERSION     DBFIFS010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.423"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            241..315
                     /gene="tap"
                     /locus_tag="N7A07_004954"
     CDS             241..315
                     /gene="tap"
                     /locus_tag="N7A07_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU2479418.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            308..1165
                     /gene="repA"
                     /locus_tag="N7A07_004955"
     CDS             308..1165
                     /gene="repA"
                     /locus_tag="N7A07_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU2479419.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          349 a          384 c          392 g          340 t
ORIGIN      
        1 tgtgatgatg ggcttacatt cttgagtgtt cagaagatta gtgctagatt actgatcgtt
       61 taaggaattt tgtggctggc cacgccgtaa ggtggcaggg aactggttct gatgtggatt
      121 tacaggagcc agaaaagcaa aaaccccgat aatcttcaga aagtttggcg actgagaaga
      181 ttaccggggc ccacttaaac cgtatagcca acaattcagc tatgcgggga gtatagttat
      241 atgctcggaa aagttcaaga cttctttctg tgctcgctcc ttctgcgcat tgtaagtgca
      301 ggatggtgtg actgatcttc aacaaacgta ttaccgccag gtaaagaacc cgaatccggt
      361 gttcacaccc cgtgaaggtg ccggaacgct gaagttctgc gaaaaactga tggaaaaggc
      421 ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg gcacatgccc gttcgaaggg
      481 actgcgtcgg cgcatgccac cggtactgcg tcgccgggct attgatgcgc tgctgcaggg
      541 gctgtgtttt cactatgacc cgctggccaa ccgtgtccag tgctccatca ccacactggc
      601 cattgagtgc ggactggcga cggagtctgc tgccggaaaa ctctccatca cccgtgccac
      661 ccgggccctg acgttcctgt cagagctggg actgattacc taccagacgg aatatgaccc
      721 gcttatcggg tgctacattc cgaccgacat cacgttcaca ccggctctgt ttgctgccct
      781 tgatgtgtct gaggatgcag tggcagctgc gcgccgcagc cgtgttgaat gggaaaacag
      841 gctgcgcaaa aagcaggggc tggataccct gggtatggat gaactgatag cgaaagcctg
      901 gcgttttgtg cgtgagcgtt tccgcagtta ccagacagag cttaagtcac ggggaataaa
      961 gcgtgcccgt gcgcgtcgtg atgcgggcag ggaacgtcag gatatcgtca ccctggtgaa
     1021 acggcagctg acgcgtgaaa tctcggaagg gcgcttcact gctaatcgtg aggcggtaaa
     1081 acgcgaagtg gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc gcaattacag
     1141 ccggctggcc acagcttccc cctgaaagtg acctcctcag aataatccgg cacgcgccgg
     1201 aggcattcgc acgcctgaag cccgccggtg cacaaaaaat cagcaccaca tcccaaaaac
     1261 aacctcatca tctaccttct ggtgcatccg gtcccccctg ttttgataca aaacacgtct
     1321 cacagacggg caattttgtt tatccacatt aaactgcaag ggatttctct gtaagattac
     1381 aaccgttcat gtcataaagc accagccgcc aggcttacag ggtgcaatgt atcttttaaa
     1441 cacccgttta tatctccttt aaact
//
LOCUS       DBFIFS010000422         1429 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.424, whole genome shotgun sequence.
ACCESSION   DBFIFS010000422 DBFIFS010000000
VERSION     DBFIFS010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.424"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..586
                     /gene="rfaL"
                     /locus_tag="N7A07_004956"
     CDS             <1..586
                     /gene="rfaL"
                     /locus_tag="N7A07_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU2479420.1"
                     /translation="RGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEM
                     TAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEW
                     TFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKNKYRSPYNAYYIIL
                     LSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(622..>1429)
                     /locus_tag="N7A07_004957"
     CDS             complement(622..>1429)
                     /locus_tag="N7A07_004957"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2479421.1"
                     /translation="TQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQS
                     YLANADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQS
                     FEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHC
                     YYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMT
                     ADSISSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          436 a          269 c          257 g          467 t
ORIGIN      
        1 cagaggggcc tggctttccg tgttagtcat tggattaata tggattctga tgtttaaaca
       61 atggaagtta ctattagtag gagtaatggt tgccatcatt gcattgtcgg ttattttcac
      121 acataaggag atgaccgcaa agctaacgta taaacttcaa caaactaata gttcttatcg
      181 ctatgcaaat ggtactcaag gcagcgcact cgatctaata ttagaaaatc ctgttattgg
      241 ttatggttac ggtaacgttg catataaaga tgtctataat aaacgtgtca ttgattatcc
      301 agaatggacc tttagacaat caatagggcc acataatttt gcgctattca tctggtttgg
      361 cactggttta ttagggctgg taagtcttat gatgctatac tgtgcaatat tgaaagagtg
      421 tataaaaaat ggcgtcaaga ataaatatcg ctcaccatat aatgcatatt atataatctt
      481 actatctttt ataggttatt ttgttatccg tggaaacgta gaacaaattg aaccaaattt
      541 attaggcgtt tacgccggct tattattagc gatgaaaaac aagtaaaaaa taaaaaaggc
      601 tgcataatgc ggccttttta tttattcaaa ccaattatga ataacctctt cgaatctttg
      661 agttacgcca tcccaactgt agtgatcaaa cacaaagtcc tgaccttgct ttgcaactgc
      721 tgttaattca ggattcgcta acgtttttaa aatatcgctg cttatggagt ctgcagtcat
      781 aggttctttt aaatggaatc ccgtagtatt ttctttgaca aattccgtca tccccccacg
      841 cgtactcact aaaacaggct taccagcacc catagcttca attgccacca tgcaaaatgg
      901 ttcctggaac tgggaaggaa tgacgactaa atctgccagt gggtaataac aatgcatctt
      961 ttcaggcggt atgcttccta acataatgca acgatccttc aaacgttttg caatttccct
     1021 gacattgcgt tgatatgcac ctttgtcact tttactcatt tctgtataat caccaatcac
     1081 aactaatttt aaatttttgt gagcagcggc caatttttcg aatgattgca tcaacagcaa
     1141 tatgcctttg tcagggacga tacgcccagc ataaaaaata attttctctt caggggagat
     1201 attaagttcg cttctagtga tcggctgaaa attagattga taggtttcta aatcaattcc
     1261 atttggtaca atctcaatat ctgcatttgc taaataagat tggtaatact ttttcaagta
     1321 taaactaggc actatcattt taacattttg ctcaagcaat tttggttcaa atgcattatg
     1381 catatgtata accactcttg cctgaggagc tcgcttacga atttgagtg
//
LOCUS       DBFIFS010000423         1424 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.425, whole genome shotgun sequence.
ACCESSION   DBFIFS010000423 DBFIFS010000000
VERSION     DBFIFS010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.425"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            64..>1424
                     /locus_tag="N7A07_004958"
     CDS             64..>1424
                     /locus_tag="N7A07_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU2479422.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSG"
BASE COUNT          414 a          256 c          358 g          396 t
ORIGIN      
        1 tttaatatta atgcgtcatg tcacgcaggg gttgctgctg tctttattta gtttgagaag
       61 ataatgcaat tacgatttaa ttttggtttg ctgctcattc tttgttgttt tcaggttgca
      121 gcgaaagaca gtcttgataa tttgatcaag cagcaaaaaa atgcagatga aagcacgttg
      181 cgcgcgcata aattagaaaa aaaagatgtt tactcaaatg cacaacgtaa tgcgtttact
      241 ctgggtgatc tgccgcagga aaagaattgc tttatcatta atcggattga actggaagat
      301 aactttctgg gtggtcagtt gatttctgaa gtgcaaaaag cgattgccgg acgttgtgtc
      361 ggtagtgctg gtgtgcaaaa aattgcagct gccttgcagg attattatat taatgcggga
      421 tatatcacaa cgcgaattag tatcccttcg caggatattt ctaaaggcaa gctacgtttc
      481 caggttaatg ccgggaagat agaaaaaacg attgtcgaag gtaatgatat cagtgaatgg
      541 atgttacctt ttcagcgtaa cgatattctc aatattcgtg atattgaaca ggggctggaa
      601 aatcttcagc gagtacctga cgttgatgtg aaaattaata ttgaaccggg aacacgggat
      661 ggttacagca tcgttcatat tgatacacac cgggggaaaa actggagcgt tcgggcaagt
      721 tacaacaatt ggggcgatga agaaacgggc cgttaccaga caacctcggt gggttatctg
      781 tttaatccag cgaaaatggg ggatctgttt tatctcgccg gtacgcgtag tactaccgga
      841 cagtatgaaa acgtcagtag ttattattcc attccggtgg ggtactggga gtacggcttt
      901 ttctatagca aaagtaaatc gcagcaggtg attccgcttt cgtacatctc gctggattat
      961 gttggcatca gcgaatactt gagcgcaaaa gcgacgcata ccgtttatcg cgataaaaca
     1021 aaaaagttcg ccggtagtgc agaactcatt cgacgaaagt cgaattacac gcttaatggt
     1081 gaggagctga cattacaggc gcgggatatg ggaaatatca agctggggct aaattataag
     1141 caacagttac ccggcgcgtt ctgggattca accctctcgt ggcaacgctt tttaacctgg
     1201 ttcggtggtg aaaaaacgcc ggatatgcaa tatggcgacg ttagcccggt aagcaatatc
     1261 tttaattttg agggtaacta tacccgacaa atagagaatg gatattacaa taccgctttt
     1321 tttgcccagt acgcaccgcg tgaactgact ttgcaagata aaattacagt tggcgatcgt
     1381 tggagtattc gcgggtttga aaatagcgta ggtttgtcag ggaa
//
LOCUS       DBFIFS010000424         1423 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.426, whole genome shotgun sequence.
ACCESSION   DBFIFS010000424 DBFIFS010000000
VERSION     DBFIFS010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.426"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(172..1224)
                     /locus_tag="N7A07_004959"
     CDS             complement(172..1224)
                     /locus_tag="N7A07_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU2479423.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          459 a          324 c          302 g          338 t
ORIGIN      
        1 ctcacccagt gggctgatct ttataaaata tgaacaggtt gaaaaaaaaa cagtaacttt
       61 gctgtttttt atacttaacc actatgcatt aatgctgcgt tatttcattc tacctaagaa
      121 aaaccagcgt tacgcaaatg gttaacgctg gttttatccg gtacgttgca attatttttt
      181 agcagaaccc gcttctaata ttgaaacgat tgagaacaac gtaaagcata ccgctcccaa
      241 accgaccaga acccgcgaag gaataaaata tcccatatcg gtctgcgcca tttccgcaag
      301 aaacgatgct aggaaaaggc aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt
      361 ggctaacgaa caggtacgtc tccagaccag tgccagtagc cagacttttg agaatatgct
      421 gtaacagatc attcccaagc aaatgaggat aataccggga gccagtcggg cgcttgcatc
      481 agaactgact aagacgtata tgccctgcag caccgtgatt gagccgagaa aaataaccca
      541 ataacaccac agccagtgct ctttcgttga aaacgtatta cgtgtttgat ggacgattgt
      601 ggcaacaagg ccaattagac aggcacagat tgcggttaac ccgagcaata cgtgaccggc
      661 gacataatgc ggagttatgt cggcactacg taacagcgta atagaccaaa tgaaaccgag
      721 cagggtgagt aacacgggaa cggcaatgag gcaattaccg attaatgaag aataagcctg
      781 taccggtgtt ccgtcgctct tgctccccgc tgcgttttta ggaatgagca gaaagtgacc
      841 ggatgacgct gccaccgtcg atacacaggc ggcaatcata ccaacgccga aaataacatg
      901 gccggcgaca aactcgtctg ccatcacatc attacctgct aacagtgccc aaccccatat
      961 catggtgata attgaccccg catagccaat aatggggaac aatgtattgt aaaacgtatt
     1021 aacgccgcgc gtgagctgcg aaatgataat aaatgcggta gtgaataatg ccaggcatat
     1081 ggctgccaga gaaatgagca catgacccgc gacgaaatac tcgctttgcc ctgaactttc
     1141 cagcacaaag ccacctaacg cgatacaaat caagcccata attaaaggga ttaatttgaa
     1201 aagccacccg atatatatgt tcactttaaa ctcctcaggt atccctgcta atattgaaat
     1261 gtgcattaat gataatttca gcctgttaat acaatgacga gatatcatta aagacaaatg
     1321 catagcataa ctatagcggg gtttactttg cagagcgcta cttttcagcg ttcaaaaggc
     1381 catgataaat aaatatttat cctaagatta attcttattc tgt
//
LOCUS       DBFIFS010000425         1420 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.427, whole genome shotgun sequence.
ACCESSION   DBFIFS010000425 DBFIFS010000000
VERSION     DBFIFS010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.427"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            56..601
                     /locus_tag="N7A07_004960"
     CDS             56..601
                     /locus_tag="N7A07_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2479424.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            576..>1420
                     /locus_tag="N7A07_004961"
     CDS             576..>1420
                     /locus_tag="N7A07_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2479425.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNPL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIR"
BASE COUNT          339 a          344 c          403 g          334 t
ORIGIN      
        1 aagtcatttt cgggcacttt catgtcggac cctgtatttg ttgtgagact gtttcatgaa
       61 ggttaataaa aagaaacttg ccgaaatttt caacgtggat ccgcgaacga ttgaacgctg
      121 gcagtctcag gggctccctt gcgtctccgg aggtggtaag ggcgttgaat ctgtatttga
      181 taccgccacg gcaattcagt ggtatgcgca gagggaagct gatatcgaaa atgaaaaact
      241 ccgtaaagag gttgaggatt acagggctgc cagcgaggca gatctccagc ctgggactat
      301 tgagtacgaa cgccatcgac ttacgcgtgc gcaggctgac gcccaggagc tgaagaatgc
      361 cagagactcc gcagaagtgg tggaaaccgc attctgtact ttcgtgctgt cacggatcgc
      421 aggtgaaatt gccagtattc ttgacgggat ccctctctca gtacagcggc gttttccgga
      481 actggaaaac cgacatgttg atttcctgaa acgggatatt atcaaagcca tgaacaaagc
      541 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcagattg
      601 atattctgcg gcgtgatgta cgcgccgggc tgcgagccct gttcaggccg gagccacaga
      661 ctgccgttga atgggcggat gccagttact atctcccgaa agaatccgca taccaggaag
      721 ggcgctggga aacactaccc tttcagcggg ctatcatgaa tgcgatgggc agcgactaca
      781 tccgcgaggt gaatgtggtg aagtctgccc gtgttggtta ttcaaaaatg ctgctgggtg
      841 tttatgccta cttcatagag cataagcagc gtaaccccct tatctggttg ccgacggatg
      901 gtgatgccga gaactttatg aaaacccacg tcgagcctac catccgcgat attccgtcgc
      961 tgctgtctct ggccccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     1021 gtttcaccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     1081 aaaagtcggt tgatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     1141 aggaaggctc tccgacgttc ctgggtgaca agcgtattga aggctcggtc tggccaaagt
     1201 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     1261 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     1321 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     1381 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg
//
LOCUS       DBFIFS010000426         1418 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.428, whole genome shotgun sequence.
ACCESSION   DBFIFS010000426 DBFIFS010000000
VERSION     DBFIFS010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.428"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..585)
                     /locus_tag="N7A07_004962"
     CDS             complement(<1..585)
                     /locus_tag="N7A07_004962"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU2479426.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWP"
     gene            complement(1187..1321)
                     /locus_tag="N7A07_004963"
     CDS             complement(1187..1321)
                     /locus_tag="N7A07_004963"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479427.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT          412 a          353 c          276 g          377 t
ORIGIN      
        1 aggccagcac agatgggaac tacagaaccc cagcttttcc gtcagacatt gttttcccac
       61 cagaatatct tctttcatga gccggcactg ttcagcacgt gatacagaca acctgtccca
      121 gcgtttatgt gaatgggtgt gcacatgaaa ttcaaccagt ccggaatcac gcatttcccg
      181 gacttcagac cagcgaagca tgacctcatc tgaacggtta tcggcaatca accgttcaca
      241 gtcgcggtga gaatattcct gctcctgcct gctacgtacg tttcccttac caatcagccc
      301 cgtaatgaga aaaatatgtg catgaaggtt atattccttc aggaccggcc atgctctgag
      361 ccagttatca agataaccat catcgaatgt gagcatgaca ctctttcgtg gaagcgttcc
      421 cccctgataa aaatattcaa gctcagcaga tgttaccgtc cgccagttat tatctgcgag
      481 ccacttcatc tgttcacaaa atgtttcagg tgagagtgtc acaagccccg gacaacgact
      541 gacatgatga tacatcagta cgggaagatg ccttgcattc aacataaaaa aataatcgtc
      601 tgttaagcat tctgaaaata tcctcgcgta aacaacagtg aggatccata gatatcacac
      661 atggtgatat tattgtgtaa tcccggaatg gtccggagat taccgacaac agggattttt
      721 taatattttt atcagttaat caaccagaac ttaaatttcc cttaacgcat atgctctttt
      781 taatcagatt ttctgttttt cagaaaaaac agaataccgc aatttcagta aacacagcga
      841 gagatatccg cctcagtaaa aaatcagaag attatcatcc tgatttattg cataaaacca
      901 gtcgagtagc ggaattttct gcatccggat accactcccc ctgaaaaata ctcgcacaat
      961 atcgcgccgc atgcactgat ggaccgggcc atcgtgagaa tctcttgcat cgtagatatt
     1021 aaccacacca gtgcccccca gaacaacagt aatacctgta gcagacctgc catagaggtt
     1081 aaataaccat tcatatccct cttctaccca ttcatcccga agtttcagcc agcgacgaat
     1141 ggactgtacc tcctgtttca gccgaggggc tgagttgaca aaccgtttag agccgccgga
     1201 tattcaggtt accggcctgc aaatcaatat cagacagttt tatacctaat aactctgatg
     1261 cttggacacc atgaagatac cccataagga gcaggcagtg attacgttcc gggtgtggca
     1321 ctttgaagac agcactgtgc aacttatgaa tctcacccgg gttcagatat ttttgttgtc
     1381 cattacctgt ccttatttat tgaaagtcga tattagtt
//
LOCUS       DBFIFS010000427         1417 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.429, whole genome shotgun sequence.
ACCESSION   DBFIFS010000427 DBFIFS010000000
VERSION     DBFIFS010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.429"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(229..1415)
                     /gene="ompC"
                     /locus_tag="N7A07_004964"
                     /pseudo
     CDS             complement(229..1415)
                     /gene="ompC"
                     /locus_tag="N7A07_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT          376 a          321 c          323 g          397 t
ORIGIN      
        1 tgttacggat taattcataa agaaatgcat ttgggtgttg gcttgaagag tcgctggttg
       61 atgttattgt gccaggaggg gcagggtaag ggggtgtatc gataagcttt ggcaattgcg
      121 tgtgccgcag atggagatga aatgccaata aaaaagcctg agttcgatga gggacgaact
      181 caggctagtg ttcagtttta caataacacg ccgcggcgcg ttggctgatt agaactggta
      241 gactaagccc acagccacga tatcatcggt agcgatgccg ttttctttgt agaagtcgtc
      301 gtcgttgtcc agcaggttga ttttgtaatc aacataagtg gacatgtttt tgttgaagta
      361 gtaggtcata ccaatatcaa catatttaac cagatctttg ttggtgtaat gatgattacc
      421 ctggctgtcg aagttatcac tatttacacc gcctaaatcc ttacctttag attgcagata
      481 agcgatggac gggcgcagac cgaagtcgaa ctgatactga gcaacaactt caaagttttg
      541 ggttttgtta gcgatggcat cagcatcacc gtaaccagtc atgttacggg gttcagcata
      601 catagctgct aagtaaacgt tattagcgtc gtatttagca ccaacggtcc atgcttctgc
      661 tgtttcaccg ccagcagttg catcgaagcg atctccataa cgagtgttat gagtaccttt
      721 gtgaacctga ttgtcggtac ggtcagagtt agagtaagca gcgcccagac ttaaaccaaa
      781 gtcaaagtca taggaagtgg acatactgaa ccgtcgccgt tctctttagc aagtttacgt
      841 ccattaccgt tacctgaacc ttcttgatca aataatccgc ttttctcgtt gttaccctga
      901 tactggagtg caaagttcag accatcaact tgaccaaaga aaccattgtt acgataggtt
      961 gctacgctgt tagcacgacc gttcatgaag ttgtcagcgc cagcataggt atctccacca
     1021 aattctggca gcatatcggt ccatgcttct acgtcatata cgacaccgta gttacgacca
     1081 tagtcgaaag taccattctg accaaagccg aaacctgcaa atgccagacg cgttgcggta
     1141 ttacccttgt cgctttcagg accgtttgcg ccaatgttaa attcccactg accataacca
     1201 gtgaattggt cagcgatctg agtttcacct ttgaaaccga tacgggcata tgacatgtcg
     1261 ccatcgctgc tagcatcatc agagaagtag tgcaggcccg ctacttttcc gtacaaatcc
     1321 agtttattgc catctttatt ataaatttca gccgcatttg ctgcgccagc aactaataac
     1381 gccgggacca gcattgccag aacttttctt ttcatta
//
LOCUS       DBFIFS010000428         1411 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.430, whole genome shotgun sequence.
ACCESSION   DBFIFS010000428 DBFIFS010000000
VERSION     DBFIFS010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.430"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..864
                     /locus_tag="N7A07_004965"
     CDS             <1..864
                     /locus_tag="N7A07_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2479428.1"
                     /translation="NELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELEERLGVQL
                     LVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINASQHAIDKC
                     LLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQGMVAVRITPDMEM
                     AIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQPEGQWAF
                     SDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYYPHRNVSP
                     ALRMVIDTLKI"
     gene            1101..1163
                     /locus_tag="N7A07_004966"
     CDS             1101..1163
                     /locus_tag="N7A07_004966"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479429.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
BASE COUNT          365 a          338 c          343 g          365 t
ORIGIN      
        1 aacgagctat acctgtttat gcaggtggtg cgggaaggca gctttaccgc agcggctaag
       61 cggctgggtc ttgctcagtc aggagtcagc cgttctgttc gcgagcttga agaaaggctg
      121 ggcgtccagc tgctggtgcg caccacgcgt aaactgtcgc tgacgcaagc aggcgagcag
      181 ctctaccaga agacggcatc tggatttgaa atgttagatc ttgggcttgc cacgctggcg
      241 cactatcgag agacgccctc cggcacggtg cgtatcaatg ccagccagca cgcgattgat
      301 aaatgcctgc tgccaaagct ggcggtattt aaacagcgat atcctgatat ccagcttgaa
      361 ctgataaatg agagccggtt cgtcgatatc atcgaggaaa gattcgatgc aggtgtccgc
      421 ctggggcctg aagtcagcca gggcatggtc gcggtacgca tcacgcccga tatggagatg
      481 gcaattgtgg ggactccgga acattttcgt cgctatggtt ttccgcaaac cccggcggat
      541 ttaaaggcgc atccctgtat cgcctatcag tttgccgacg gcagcgtata ccagtgggaa
      601 ctcaatcagg atgataaaaa aatcactcat caacccgaag gacaatgggc cttttccgac
      661 agctatatgg aagcagaagc cgcccggctg ggcctcgggt tggcctatgt tcctgttgaa
      721 ctggtcgcgg atgatttaga acacggtaag cttatcagag tgttgcagcg ttacagcctg
      781 cgaatggagg gattgtttct ctattaccct catcgcaacg tctcccccgc tctgcggatg
      841 gtcattgata cattgaaaat ctgaacgtta acaacgaccg actcttcact gtgcaatatt
      901 gtgtcaggta gtcactctgc gttcaattcc aggggccgct ttgagcgaga aagagcggac
      961 ttatctgtac aaggggacta tccagcaatg gagatcatcc aaaagaaaaa accacctggc
     1021 tcccctcgac aaccttaatc cgggcattta gcctcaatca acattttcat atctgtcagg
     1081 ttacttcatt atgaaaaccg atcaataccg ttgtttttgt tgcaacagta gaatacgcgt
     1141 atttatccct ccatttctat taataacggc aataaactgt tcatttcact gatatttaaa
     1201 atatgcatcc ttttcaagct gttcagcggc gtaagaaaac gtattaaata tgaattcgac
     1261 gatttaacca tttctgtctg attggtaatc atctgtgatt aatgctggtc tattgattac
     1321 tccagcatta accgcttagg gatgtattct tcctgttgat atacagccct cgaaaaagag
     1381 agaaaataaa actgtgtcca atattactct a
//
LOCUS       DBFIFS010000429         1395 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.431, whole genome shotgun sequence.
ACCESSION   DBFIFS010000429 DBFIFS010000000
VERSION     DBFIFS010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.431"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..332)
                     /locus_tag="N7A07_004967"
     CDS             complement(<1..332)
                     /locus_tag="N7A07_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HGU2479430.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAV"
     gene            complement(502..578)
                     /locus_tag="N7A07_004968"
     tRNA            complement(502..578)
                     /locus_tag="N7A07_004968"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Arg,seq:tct)
     gene            complement(679..754)
                     /locus_tag="N7A07_004969"
     tRNA            complement(679..754)
                     /locus_tag="N7A07_004969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Ile,seq:cat)
     gene            complement(783..>1395)
                     /locus_tag="N7A07_004970"
     CDS             complement(783..>1395)
                     /locus_tag="N7A07_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2479431.1"
                     /translation="KCIRELFLMHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPL
                     YLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFLSGCLSSISAK
                     VRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCS
                     RYGYGQAVAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          366 a          375 c          307 g          347 t
ORIGIN      
        1 accgccacat ccagcacatc ttccgcttca cgcgtatcgt gtttcacatc ctcaatctca
       61 cgtaacgccg cacgacgacc agttttaaag ttcgtatcca gctgaccgat aatggttgcg
      121 ctaatcgcat taatttctga cgtaaacgcg gacagcgcag gctgattacg cagtacctgt
      181 tcgatcttcg ctttctcgga tgcctcacat tcaccatctg cacaggccac caggtatgcg
      241 gcgttaatca ccacctgtgc cagatcgcgt ttttcaaact ttttaatttc cgttgccgct
      301 ctgcgggttt tctttttgaa gattccaaac atcgtgacgt tcctttgggt gggtgagcca
      361 acgcccggga gcgatctgcc cacagagaaa gtcacactga ccactccgta agctcacccc
      421 cgaaaggctc tgtggttggt atgcgccggg cgtggcgcag atacaaaaaa ggcccgccga
      481 agcgagcctg gaaaataagt gtggcgcgtt gtagtggagt cgaaccactg accgattgct
      541 tagaaggcaa ttgctctgtc cggctgagct aacaacgcag aataccgata atggaccgcc
      601 atcggggacc cggccccgca ccaacaaccc tgttatggtg tcgtctgctc ttcctgataa
      661 gctaatggcg gtttgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat
      721 gagtcgctcg ctctcaccac tgagctaaag ggccgagcca aaaaataata atcagatgaa
      781 aatcaataat caagcccttg cctggataca tatctgtctg gcgggaagcc ataatagcgg
      841 tgaaatacag aaataaagta ggaccggctt gaataaccac atttttctgc tacagcctgt
      901 ccatatccat accgggaaca taacatattt acagcaacac gcatccgctc ttccagcaac
      961 aagcgactga acatgccccc ttcatttttc agttttgtct ttaacaaact ctcactcata
     1021 tgcaactgta gagcaatcgc accaagcgtc cagcttgctg atatatctgt ctgaattatc
     1081 gccctgactt tggcacttat gctggataaa catccactta aaaacaatga catccgttca
     1141 tctgtttcaa acagagacag gcaggccatc ataagaaaca tatccgtgac ctctccggaa
     1201 aatccctggc tggtaattaa agccgcagcc aacgcaggat tgttgggttc cagcaacagg
     1261 taaagcggaa tgtcagtcag acgagtcctc gtcagcttat gctgactttc cagatattga
     1321 cttacgacgg attcgcttat atcgacaatt ttaactttgc cataatgcat aaggaaaagc
     1381 tccctgatgc atttg
//
LOCUS       DBFIFS010000430         1384 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.432, whole genome shotgun sequence.
ACCESSION   DBFIFS010000430 DBFIFS010000000
VERSION     DBFIFS010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.432"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1384
                     /locus_tag="N7A07_004971"
     CDS             <1..>1384
                     /locus_tag="N7A07_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU2479432.1"
                     /translation="LPETYDRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQ
                     DMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADG
                     TYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPA
                     NHAAQFGALVDKFRADLADMAGQCVGGSADGVPWICGDTTYFWKQKNEATYQTVYGSY
                     KNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASH
                     FSAWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGITTLLSYL
                     ASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALL
                     QKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKD
                     LNVMYHNAKVA"
BASE COUNT          333 a          339 c          446 g          266 t
ORIGIN      
        1 ctgccggaga catatgaccg tccggacccg cgtattaagc agctggcgcg ccgcagtacg
       61 gtgacaccgg gcggtgtcgc ctgtaaatat aacgacatca ttccggcgga ccattgtctg
      121 catgatgtgc aggacatgag ccgccttaac catccgaaag cggacctgtc aaaggggcag
      181 tacggaaccg tggggcaggg gctgcatatc gccaaaaaat tgctgccgtt tataccggcg
      241 aatgcgggca ttctgctggt tccgtgctgt cgtggtggtt cagcgttcac caccggagct
      301 gatggcacat acagtgacgc gagtggtgct tcggagaatt caacccgctg gggtgtggac
      361 aagccgctgt ataaggacct tatcggtcga acaaaagcag cactgaagaa gaacccgaaa
      421 aatgtgctgt ttgccgtggt gtggatgcag ggggaatttg attttggcgg tacgccggca
      481 aatcacgcag cacagtttgg tgcgctggtt gataaattcc gtgcagacct ggcggatatg
      541 gcaggtcagt gcgtcggtgg ctctgctgac ggtgttccct ggatatgcgg ggacacgacg
      601 tatttctgga agcagaagaa cgaagccacc taccagacgg tgtacggcag ctacaaaaac
      661 aaaacggaaa aaaatatcca tttcgtaccg ttcatgacgg atgagaacgg ggtgaatgtg
      721 ccgacgaaca aaccggaaga agacccggac attccgggta tcggatatta cggttcgaaa
      781 tggcgtgaca gctcagccac ctggacgtca caggacaggg cgagccattt cagcgcctgg
      841 gcacgccgtg ggattatttc cgaccgtctg gcaacggcga ttttgcgcca tgcgggaaga
      901 gtggcgctaa acgcgggggc atcatcgaca gtatcagagg tgcgcccgtc atcgccttcc
      961 ggtgcagaag ccacaggcat cacaacactg ctctcttacc ttgccagcga gtcagaggga
     1021 agcctgaaag tacagggatg gtcagccagt ggcggcaggg cagaagtggt cagcgatgcg
     1081 gagggaaccg gaggtaaggc agtgaagctg accaaggaag ccggtaaaag cagctgggtg
     1141 ctggagtacg ccgcgggcaa cggtgcggct ctgttacaga aaggggggca gattcgctgc
     1201 cgctttaagg tttcgggagc gctggctgcg aaccagtatg ttatggcgtt ttactggccg
     1261 gtatcttcac tgccacaggg cgttgccctg accggagacg gggggaataa cctgctggca
     1321 gcgttctaca tccagacaga tgcaaaagac ctgaatgtga tgtaccacaa tgcgaaagtg
     1381 gcga
//
LOCUS       DBFIFS010000431         1374 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.433, whole genome shotgun sequence.
ACCESSION   DBFIFS010000431 DBFIFS010000000
VERSION     DBFIFS010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.433"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            90..1318
                     /locus_tag="N7A07_004972"
                     /pseudo
     CDS             90..1318
                     /locus_tag="N7A07_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          363 a          321 c          396 g          294 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataatgaaa aaca
//
LOCUS       DBFIFS010000432         1369 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.434, whole genome shotgun sequence.
ACCESSION   DBFIFS010000432 DBFIFS010000000
VERSION     DBFIFS010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.434"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1237
                     /locus_tag="N7A07_004973"
     rRNA            <1..1237
                     /locus_tag="N7A07_004973"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          338 a          309 c          424 g          298 t
ORIGIN      
        1 aactaggcaa aatggtgccg taacttcggg agaaggcacg ctgatatgta ggtgaagtcc
       61 ctcgcggatg gagctgaaat cagtcgaaga taccagctgg ctgcaactgt ttattaaaaa
      121 cacagcactg tgcaaacacg aaagtggacg tatacggtgt gacgcctgcc cggtgccgga
      181 aggttaattg atggggtcag cgcaagcgaa gctcttgatc gaagccccgg taaacggcgg
      241 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacctgcac
      301 gaatggcgta atgatggcca ggctgtctcc acccgagact cagtgaaatt gaactcgctg
      361 tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg tgaaccttta ctatagcttg
      421 acactgaaca ttgagccttg atgtgtagga taggtgggag gctttgaagt gtggacgcca
      481 gtctgcatgg agccgacctt gaaataccac cctttaatgt ttgatgttct aacgtggacc
      541 cgtgatccgg gttgcggaca gtgtctggtg ggtagtttga ctggggcggt ctcctcctaa
      601 agagtaacgg aggagcacga aggttggcta atcctggtcg gacatcagga ggttagtgca
      661 atggcataag ccagcttgac tgcgagcgtg acggcgcgag caggtgcgaa agcaggtcat
      721 agtgatccgg tggttctgaa tggaagggcc atcgctcaac ggataaaagg tactccgggg
      781 ataacaggct gataccgccc aagagttcat atcgacggcg gtgtttggca cctcgatgtc
      841 ggctcatcac atcctggggc tgaagtaggt cccaagggta tggctgttcg ccatttaaag
      901 tggtacgcga gctgggttta gaacgtcgtg agacagttcg gtccctatct gccgtgggcg
      961 ctggagaact gaggggggct gctcctagta cgagaggacc ggagtggacg catcactggt
     1021 gttcgggttg tcatgccaat ggcactgccc ggtagctaaa tgcggaagag ataagtgctg
     1081 aaagcatcta agcacgaaac ttgccccgag atgagttctc cctgaccctt taagggtcct
     1141 gaaggaacgt tgaagacgac gacgttgata ggccgggtgt gtaagcgcag cgatgcgttg
     1201 agctaaccgg tactaatgaa ccgtgaggct taaccttaca acgccgaagg tgttttggcg
     1261 gatgagagaa gattttcggc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
     1321 aacagaattt gcctggcggc cttagcgcgg tggtcccacc tgaccccat
//
LOCUS       DBFIFS010000433         1363 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.435, whole genome shotgun sequence.
ACCESSION   DBFIFS010000433 DBFIFS010000000
VERSION     DBFIFS010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.435"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            288..965
                     /locus_tag="N7A07_004974"
     CDS             288..965
                     /locus_tag="N7A07_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU2479433.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            1037..1321
                     /gene="ppnP"
                     /locus_tag="N7A07_004975"
     CDS             1037..1321
                     /gene="ppnP"
                     /locus_tag="N7A07_004975"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU2479434.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          359 a          297 c          339 g          368 t
ORIGIN      
        1 aaacgctatg aggaccctga caaagagtga ccgcatcaga ctgctcggaa gggattctga
       61 gtgccactac aagggatctg cgtcacattt ttcataattc atgtttttca aataattaga
      121 atattaaaca ataacaatcc attactggaa tcatttggaa tctttacatt atgccgtgca
      181 cgtctgctgc tacgcttttt gtcatttgta gcacaagtaa gtgtcagcag tggtgcttaa
      241 cacttgcccg gtaattaacg acgaaataaa agtaaggtgg aagaacaatg agtgcgtcgt
      301 tggcgattct aaccatcggc attgtaccta tgcaggaagt tttgccgctc ctgacagaat
      361 acattgacga agataatatt tcccatcata gcctgctggg gaagttaagc cgcgaagaag
      421 tgatggcgga gtacgcgcca gaagcaggcg aagacacaat tctcacatta ttaaatgaca
      481 accatctggc ccatgtttcg cgtcgcaaag tagagcgtga cctgcaaggt gtggttgaag
      541 tgctcgataa tcagggctac gacgtcatta tattaatgag tacggcaaac attagtagta
      601 tgactgcgcg taatacgatc tttcttgagc cgtcgcggat attgccaccg ctggtttcct
      661 ctattgttga agatcatcag gtgggggtta tcgtaccggt tgaggagttg ctgaccgttc
      721 aggcgcaaaa atggcaaatt ttgcagaaac cgcctgtatt ttcattgggt aaccccattc
      781 atgattcaga acaaaaaatc attgatgccg ggaaagaatt actggcgaaa ggcgcagatg
      841 tcatcatgct ggattgtttg ggatttaacc agcgtcatcg cgatttactg caaaaacagc
      901 tcgatgttcc tgtcttgctc tctaacgtat tgattgcacg gctggctgcg gaattactgg
      961 tgtaattttg cgtgacagcc agcgcatctg gcccctatag tgaagtagat attcaactac
     1021 cagacagggc cagtttatgc ttcaaagtaa tgagtacttt tccggcaaag tgaaatcgat
     1081 cggcttttcc agcagcagca ctggtcgcgc cagcgtgggt gttatggttg aaggcgaata
     1141 caccttcagc accgctgagc cggaagagat gacggtaatc agtggcgcgc tgaatgtgtt
     1201 actgcctgac gcgaccgact ggcaagtgta tgaagccggt tcggtgttta atgttcccgg
     1261 tcacagtgag tttcatctgc aagttgccga acccacctct tatctgtgcc gctatctgta
     1321 attcctcgcc ttccgcttga acgggagggc atttttctga aat
//
LOCUS       DBFIFS010000434         1363 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.436, whole genome shotgun sequence.
ACCESSION   DBFIFS010000434 DBFIFS010000000
VERSION     DBFIFS010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.436"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1037
                     /gene="escC"
                     /locus_tag="N7A07_004976"
     CDS             <1..1037
                     /gene="escC"
                     /locus_tag="N7A07_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU2479435.1"
                     /translation="EAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILK
                     TMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELD
                     IEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNAS
                     NFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGT
                     LLRVTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMT
                     TEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIK
                     ATPIKSASSE"
     gene            1051..>1363
                     /gene="cesD"
                     /locus_tag="N7A07_004977"
     CDS             1051..>1363
                     /gene="cesD"
                     /locus_tag="N7A07_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU2479436.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SY"
BASE COUNT          441 a          267 c          273 g          382 t
ORIGIN      
        1 aagaggcaca gtctaaagcc aaaaataaag atgttgttaa ggtctttaaa ctgaactatg
       61 cgtcagcgac agatataaca tacaaatacc gtgaccaaaa tgttgtcgtc ccaggtgttg
      121 tctccattct gaagacaatg gcaagtaatg gttcacttcc cagtaccggt aaaggtgccg
      181 tcgagcgaag cggtaacctg tttgataaca gtgtaacgat ttccgctgat ccacgtctta
      241 atgcagttgt tgtaaaagac cgcgaaataa cgatggatat ctatcagcaa ttaatttctg
      301 agcttgatat tgaacaaagg caaattgaaa tttctgtctc aattattgat gttgatgcaa
      361 acgatcttca gcagctcggc gtaaactggt ccggtacgtt aaatgcagga caaggtacga
      421 ttgcctttaa tagctctacc gcacaggcta atatcagctc ttctgtgata agtaatgcca
      481 gtaattttat gattagggtg aatgcgttac aacagaactc gaaagctaaa atactctctc
      541 agccttcaat tataacctta aataatatgc aggcgattct tgataaaaac gtaacgtttt
      601 atacaaaagt ctccggagaa aaggtggcgt ctctggaaag tattacaagc ggaacattgc
      661 tgagagtgac accacgtata cttgatgaca gctcgaatag ccttaccgga aaacgtcggg
      721 aacgagttcg tttgttactt gatattcagg atggcaatca atcgactaac caaagcaatg
      781 ctcaggatgc cagcagcacc ttacctgaag tacaaaattc agaaatgaca acagaagcga
      841 cattaagtgc aggagaaagt ttattacttg gcggatttat ccaagataaa gaaagttcaa
      901 gtaaagatgg tataccgctg ttaagcgaca ttcctgttat tggtagttta ttctccagta
      961 cggtaaaaca aaaacatagt gttgttcgac tctttttaat taaagcaacc ccgataaaat
     1021 ctgcatctag cgaataataa atgaggcttt atgagcagga aatttagctc tctagaggat
     1081 atttatgatt tctaccagga tggtggcaca ttagcgtcat taacaaatct gacacaacaa
     1141 gatctcaatg accttcattc ttatgcctat acagcatatc aatctggtga tgttataacc
     1201 gcaagaaatc tattccattt gctcacatat ctggaacact ggaattatga ctacacctta
     1261 tctctgggct tatgtcatca gcgtttatca aatcatgaag atgcacaact gtgtttcgca
     1321 cgctgtgcaa ctttagttat gcaagatccc agggcatctt att
//
LOCUS       DBFIFS010000435         1338 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.437, whole genome shotgun sequence.
ACCESSION   DBFIFS010000435 DBFIFS010000000
VERSION     DBFIFS010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.437"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..>1338)
                     /locus_tag="N7A07_004978"
     CDS             complement(<1..>1338)
                     /locus_tag="N7A07_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479437.1"
                     /translation="EYGYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDS
                     TLTAWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVF
                     YTRIQHGEPLVESRYLYDPLGRRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLT
                     TVQTDTTRIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPA
                     ELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHR
                     GLPLALISEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRY
                     YDPLQGRYITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAW
                     DILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD"
BASE COUNT          221 a          452 c          339 g          326 t
ORIGIN      
        1 aatctctaat ctctttttca taacccagcc ctttcgccga tcggctaaca ccagcgtcat
       61 ttaatttaga cactcgacaa aatgccatac aatggaaaaa ttgatcagtc ccacctaaat
      121 ttaatctctt catatcttca taagtatcag ataatatatc ccatgctctt tgatggttta
      181 gttgatcttt tcttcttatt agcgctacat ctgcgggact taaccctaat ggatcaatac
      241 cattcaccgg attcagcgga tacgcataca gactccatcc cccctccagc cctatcgggt
      301 cctgagtgat ataccgcccc tgcaacggat cgtagtaccg gttccggttg tagtacagcc
      361 ccgactcctt atcatactgc tgccccggca gccggtacgg ctggtgcagg tgatgcgggt
      421 tctcctcatt cagctggttg ccccattcat catactcccc gctccacgcc gtattgccgt
      481 cttcgctgat gagcgccagc ggcaggcccc ggtggtcgca gtggtaaaga tgaacttttc
      541 tcgccggtgt gtattccggc tccacctgtc tggccagttg ctccaccgtc agcccgcact
      601 gcgcaagcca cgcccggctt tcactgctca cgcggtctgc ccggatttct tcctccagtc
      661 tgtccagcag ccgcaccagt tcagccggaa ataccacacc gtgcccgtcc tcgctccctt
      721 cctgctgcag cttctccgcc aggctgcggc gctgcgcttt ctcccgctca ccgttgtctg
      781 tttcgatgcg gatgagcggc gcgaagcttc ccggctcgta taccgtctgg atacgcgtgg
      841 tgtcggtctg taccgtcgtc agccggtcgc cgtcccaccc gtaccacgtc acctccggtt
      901 tccgcgacag cgacatccat cccgtcaggt cacgcccgcg acgccacacc cgtttccccg
      961 tccggcggcc cagcgggtcg tagaggtagc ggctctcgac cagtggctcg ccatgctgta
     1021 tccgcgtgta gaacaccagg cggtgctggc tgtcgtagtg gtagtggtgg gtccgctcgt
     1081 cgtccgtccg tatcacaccc gccgggatgc ggtccgtctt ctccgtcagc ctgccgtatt
     1141 catcgtgtcg gtagacatag tgcgcatcct ccgcgatgcg gttatccggc cacgccgtga
     1201 gcgtgctgtc cgggtgtagc tccgggtccg gcagccggtt tcccgccggg tcggtcgcat
     1261 acgggatgcg gatatccagg tctgatgcaa gggtgcgcac gctctccagc ctgcccgtgg
     1321 cgctgtagcc atattccc
//
LOCUS       DBFIFS010000436         1332 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.446, whole genome shotgun sequence.
ACCESSION   DBFIFS010000436 DBFIFS010000000
VERSION     DBFIFS010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.446"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            738..806
                     /locus_tag="N7A07_004979"
                     /pseudo
     CDS             738..806
                     /locus_tag="N7A07_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            865..>1332
                     /locus_tag="N7A07_004980"
     CDS             865..>1332
                     /locus_tag="N7A07_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479438.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT          444 a          265 c          297 g          326 t
ORIGIN      
        1 ttattgatgt aactcgctaa accatgcagt tatgatccct gccgcataac cttcatcagc
       61 cacattttca aaaataaatt tccttatata tcagaatcat acttcgtaga gttaataaat
      121 caccaaaatt cgtaccaata gttcttgata atgtcgaact attggttcat tattatcatc
      181 gtcagcagga cgcattactc accagggcgg tgaatataca acgattcgaa tatgaatcta
      241 cggcgctgac aaagcgcaat aaccaaagtg aactttgggg tgtggtgaag ggttcatgga
      301 cgggaatatg tcgcacgtaa agcggcgagg cctgcgggac tattgccgaa ttgaagtagg
      361 ccgaaacagg tcgaaatggg tctcccacct accacaccac caaagttcat caggaggtct
      421 atatgacacg cagaactcag ttcaaaggca attcacgttc tcgtcgtcgt gagcgtttaa
      481 aggcaaaggc attagctaac ggcgtactgg cccgcgaaga agcaataagt tcagaagtat
      541 tacaccgccc tactctaagc agagcgcaga ttcaggctaa aggtactcac gaaacgcctg
      601 agcgcataga agacgctaag ccaattaagt tcatggcaca ggacgtgatc tggcaacaga
      661 aagaatacag acgcaatctg gagcgagcgg ccattgtgta cgcgaatgag tttggacata
      721 agcaaccaga aactggtgta tgtcttccaa acgtagccat ttacgcggca ggctaccgga
      781 aatcaaaaca actgacggcg aggtgacttg tgttggtcgc cagaaaatga aattaggcag
      841 caaaccactt atttgaggtg agatatgaca aaatcatgga gcgtaccttt tcctgaatca
      901 gaaactgaac atgatggaat gcctgttttc tggagattcc aggcgacagt tgaagaagat
      961 gggataaaaa tattcgcact tcaatatata gcttttcatc agacagagca ttatgcatgg
     1021 ttggttcctg cgcattggat tgttaatttt aaaccagcac caaatcagtg gttacaggaa
     1081 tggaaacaaa ggagaaatag atatgcaatt aagaaagtag caaaaaatgc agaaagatct
     1141 tttgcattcc caacgaagaa acttgccatt gaaagtttat tgcgccggaa gaaataccat
     1201 ttaatgagaa tcaaacaaga tttggctgtt gtatcaactc ttgttgatgg tatgaagaat
     1261 attgatacat caacaccaga tattgaatat aactttggac acaaccaaga aacagaaaat
     1321 tgggtatttt at
//
LOCUS       DBFIFS010000437         1325 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.447, whole genome shotgun sequence.
ACCESSION   DBFIFS010000437 DBFIFS010000000
VERSION     DBFIFS010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.447"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1325
                     /locus_tag="N7A07_004981"
     CDS             <1..>1325
                     /locus_tag="N7A07_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2479439.1"
                     /translation="VDNGASFEPLSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDT
                     PRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQG
                     EPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVE
                     TAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFF
                     KGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQI
                     QKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMIN
                     PANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISG
                     NVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPER"
BASE COUNT          326 a          335 c          395 g          269 t
ORIGIN      
        1 gtggataacg gggccagctt tgagccactg tccggttcgc tgaacagcgt catcccgccg
       61 gctgtgcagc acctgacggt ggaggtgagc gcggctgacg gtcagtatct ggcacaggcg
      121 aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca gtctgcgcct gaccagtggt
      181 aagggaacgg atgccagact ggtgaccacc gccatcaccg cagacacgga gcaccgtttc
      241 agcggcctgc cgctcgggga atacaccctg acggtgcggg cgataaacag ctatggccag
      301 cagggtgaac ctgccaccac caccttccgg attgccgcac cggcagcacc gtcgcggatt
      361 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgccgt ttatgacccg
      421 acggtacagt ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa
      481 accgcagccc gctatcttgg ctcggcgctg tactggatag ctgccagtat caatatcaaa
      541 ccgggccatg attattattt ttatatccgc agtgtgaata ctgttggcaa atcggcattc
      601 gtggaggctg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaagga
      661 gaaatcggga aaacacatct ggcccaggag ctgtggacgc agattgataa cggtcagctt
      721 gcgccggacc tggctgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcag
      781 accgtcaata aaaaactgga aaatcagagt gcggcaatcc agcagataca gaaagttcag
      841 gttgatacaa ataataacct gaacagcatg tgggccgtga aactgcagca gatgcaggac
      901 ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc aggaatgcag
      961 agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa tggcaacaca
     1021 aagccgatgt ttgttggtca gggcgatcag atatttatga atgaagtgtt cctgaaatat
     1081 ctgacggctc ccaccattac cagcggcggt aatcctccgg cattttccct gacaccggac
     1141 gggcggctga cggcgaaaaa tgccgatatc agcggtaacg tgaatgcgaa ctccgggacg
     1201 ctcaacaacg tcacgattaa cgagaactgt cgggttctgg gaaaattgtc cgcgaaccag
     1261 attgaaggcg atctcgttaa aacagtgggc aaagctttcc cccgggactc ccgtgcaccg
     1321 gagcg
//
LOCUS       DBFIFS010000438         1322 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.448, whole genome shotgun sequence.
ACCESSION   DBFIFS010000438 DBFIFS010000000
VERSION     DBFIFS010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.448"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1185)
                     /locus_tag="N7A07_004982"
     CDS             complement(<1..1185)
                     /locus_tag="N7A07_004982"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU2479440.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDL"
BASE COUNT          329 a          345 c          334 g          314 t
ORIGIN      
        1 gaggtcgtac agggtgtatc ccggatcttc accctctttc agtttgaagc aaaccgccgg
       61 gatgccttcg tccggacgac ccgtacagat gaactcatac ggccccagtt tggcgatttc
      121 atccgccaga taagcggcaa cctggtaaga ggcgttctgt actttggtat agccttcacg
      181 accgaggcgc aggaattcat aatactgcgc aatcacctga cccgccgggc gggagaagtt
      241 gatggcgaaa gtaccaatct gaccaccgag gtagtcaacg ttgaacacca gttcctgcgg
      301 cagcgcttct tcatcacgcc agatcaccca gccgcagccc agcggagcca gaccgaattt
      361 atggcctgaa gcactgatcg atttcacacg cggcaggcgg aagtcccaga cgatatccgg
      421 ggcgacgaac ggtgccagga agccaccgct ggcagcgtcg atgtgcatgt cgatgtcgat
      481 accggtgtcg gcctggaatt tatccagtgc atcgtgcagc ggttgcggga actcatagtt
      541 accggtgtag gtcacgccga aagtcggcac cacgccgatg gtgttttcgt cgcaggcttc
      601 aatcatgcgt ttcgggtcca taaacaactg accggggcgc atagggatct cgcgcagctc
      661 cacatcccag tagcgagcga atttatgcca gcagatttgc accggaccgc acaccaggtt
      721 tggtttatcc gttggtttgc ctgcagcttc catacgcttg cgccaacgcc atttcatcgc
      781 catcccgcca agcatacaag cctcggaaga accaatggtg ttggtgccaa cggcctgacc
      841 atttttcggc gcaggcgcat gccacagatc ggcaaccatg tttacgcaac gcaggtcgat
      901 ggctgcggat tgcggatatt cttctttgtc gatccagttt ttattgatcg acaaatccat
      961 caatttatgg acgttttcgt cgtcccaggt ctggcagaaa gtggccaggt tctgacgagc
     1021 gttgccatca agatataatt catcattgat aatctggaat gcgacatcat cgcgcatttc
     1081 gtgcagcgga aatcgttttg actccgcgat agtagaaatg gcctttgcgc caaaacgtga
     1141 atcgagtagt tctgagcgga aatccgttaa cagcttctgg tccatttcga actccttaaa
     1201 tttatttgaa ggcaataaaa aagtaggatt tatccgcaat ggaagcaagg cattacaggc
     1261 ttaatttaaa taacaaaatc ctaagcagaa aaatgatctt ttataatata aacatttaat
     1321 at
//
LOCUS       DBFIFS010000439         1319 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.449, whole genome shotgun sequence.
ACCESSION   DBFIFS010000439 DBFIFS010000000
VERSION     DBFIFS010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.449"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            93..656
                     /locus_tag="N7A07_004983"
     CDS             93..656
                     /locus_tag="N7A07_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2479441.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            653..>1319
                     /locus_tag="N7A07_004984"
     CDS             653..>1319
                     /locus_tag="N7A07_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU2479442.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQ"
BASE COUNT          313 a          340 c          413 g          253 t
ORIGIN      
        1 ccaggcagca gttaacattt gcggcatgag acgccgggaa aaatgggctg aaccataccc
       61 ggctgagtgc gttctggacc cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg
      121 cgctggcgct catgtccggg aaaaacaaaa cagcgtcagc ccttgccgcc ggttattcgg
      181 cgaagacagc cagggttaaa ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta
      241 tagcccgtaa acagtgcgag acggtggagg tggatgaggt tcctgtttat cggcagaaaa
      301 aatcagagcc ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg
      361 aaacaaatcc ggagatgcca ccgcccgtgg tgatatctcc tggtattgag tatatggagg
      421 acggtcttcc cgatccggtg aaagcgatgg ggcgtcttct ggtggagaac attaataccg
      481 accccaggct ggcgctggat gcggcttata agctggcgca gttcacgcac cacaaaaaag
      541 gggatgccgg taaaaaatcg gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg
      601 cggtgccacc accaccccgc ctggtggtga ataatgataa tgagggcaac ggatgatacc
      661 tgtgtggagc acggcctgcc cggactgggc agagcgcctg aaaaaggggc tgtcgattat
      721 tccggctccg atttatccgg agcaggccgc acatgccctg gcgattttta aacaactgcg
      781 gattgtggat gcaccgggca gcccgacgtt cggggagtcc tgcgcacagt gggtgtttga
      841 cctggtggcg gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc atatcaagga
      901 agtttttatc cttatcccca agaaaaacag caagtccacg ctggctgccg ggatcatgat
      961 gacggcgctg ttactgaact ggcggcaggc ggcgggctac accattctgg ccccgaccgt
     1021 ggaggtggcg gctaacgcct tcaaccctgc cagggatatg gtacgacggg acgatgatct
     1081 ggatgacctc tgtcaggtgc agacacatat ccggaccatc acccacaggg tgacggacac
     1141 caccctgaag gtggtggctg ccgatccgaa tacggtatcc ggtatcaagt ccgtggggac
     1201 gctgattgat gaactgtggt tatttggcaa gcagtacaaa gcggaggaca tgttacgtga
     1261 agccataggc ggccttgcct cccgcccgga agggtttgtg gtgtatacga ccacccagt
//
LOCUS       DBFIFS010000440         1314 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.450, whole genome shotgun sequence.
ACCESSION   DBFIFS010000440 DBFIFS010000000
VERSION     DBFIFS010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.450"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            297..458
                     /locus_tag="N7A07_004985"
                     /pseudo
     CDS             297..458
                     /locus_tag="N7A07_004985"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="N7A07_004986"
     CDS             442..507
                     /locus_tag="N7A07_004986"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HGU2479443.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="N7A07_004987"
     CDS             623..880
                     /locus_tag="N7A07_004987"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2479444.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..>1314)
                     /gene="nleF"
                     /locus_tag="N7A07_004988"
     CDS             complement(927..>1314)
                     /gene="nleF"
                     /locus_tag="N7A07_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HGU2479445.1"
                     /translation="LEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGMTIE
                     DRDSYIRDLFFLHSLKEKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCLVEG
                     CSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          385 a          313 c          267 g          349 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt ttctttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagc
//
LOCUS       DBFIFS010000441         1313 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.451, whole genome shotgun sequence.
ACCESSION   DBFIFS010000441 DBFIFS010000000
VERSION     DBFIFS010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.451"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1313
                     /gene="espL1"
                     /locus_tag="N7A07_004989"
     CDS             <1..>1313
                     /gene="espL1"
                     /locus_tag="N7A07_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HGU2479446.1"
                     /translation="AFFSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESI
                     QQHVPLVRDAEFRALLRHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDN
                     HTLSVRLRIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQT
                     WADRYVRNCAIAILPLLPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITL
                     FRQLPILPEKEIIEIITAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHED
                     NLVKLLQTKSANETPGLYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKI
                     HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIA
                     MSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAV"
BASE COUNT          382 a          283 c          263 g          385 t
ORIGIN      
        1 tgcatttttt tcgcaatctc cttctgacgc tattttttgt cgccatctgt cattgcaata
       61 cgcacttgat tcattgagaa atggaaaagg caaggtcaac ctgattaaac attactcctc
      121 cgttgaatcc atacagcagc atgtcccctt agtccgggac gcggagttca gagcattact
      181 tcgccatcct cctgcaggga gtcgcgttat cgcgagtaag gattttggct tcgctttaga
      241 tattttcttc tgtcgaatga tggcaaacaa tgtcagtcat atgtctgcga ttttatatat
      301 agacaatcat actttgtcag taaggctacg aataaagcag tcggcgtatg ggcaattaaa
      361 ttatgttgtg tccgtttacg acccgaacga taccaacgtt gccgtcagag gcacccacag
      421 gacagcacgg ggctttctct cgcttgataa gttcatcagt tcaggtcccg atgctcagac
      481 ctgggctgat aggtatgttc gcaactgtgc aattgctatt ctgcccctat tacctgaggg
      541 agttccaggg gctattttca cgggtatcgc gacacgaatg ccatttgccc ctatacatcc
      601 atcggcaatg ttgttaataa tggccacagg ccagactcaa cagcttatta cattattcag
      661 acagttgccc atactccctg aaaaagaaat cattgaaata ataactgcgc agaatagcat
      721 tggtacacct gctttatttt tggctatgat gaacggacat actgacaacg tgaaaatatt
      781 tatgcaagaa attcagtcac tggtagataa tcacatcatt catgaagata atctggttaa
      841 attgttgcaa actaaaagtg ctaacgaaac acctggactt tatatctcca tgttgtatgg
      901 attcgatgag ataatcgata tctttctgaa tgcattaacc actcctatag cacaagagct
      961 tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg gtgagccagg
     1021 attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc tctctaaaat
     1081 taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt tattaaaagg
     1141 cgctacagca cagggaaccc ctgcattata catcgctatg agcaagggta atgaagacgt
     1201 cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt ttagtcaaca
     1261 tcagttattt acactgttgg ctgctaaaaa tcatgacaac atgtcagctg ttc
//
LOCUS       DBFIFS010000442         1300 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.452, whole genome shotgun sequence.
ACCESSION   DBFIFS010000442 DBFIFS010000000
VERSION     DBFIFS010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.452"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..815)
                     /locus_tag="N7A07_004990"
     CDS             complement(<1..815)
                     /locus_tag="N7A07_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2479447.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGG"
     gene            792..934
                     /locus_tag="N7A07_004991"
                     /pseudo
     CDS             792..934
                     /locus_tag="N7A07_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1129..1242)
                     /locus_tag="N7A07_004992"
     CDS             complement(1129..1242)
                     /locus_tag="N7A07_004992"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU2479448.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          321 a          376 c          326 g          277 t
ORIGIN      
        1 ctgccaccat ggcactgcgc gttaaacacg gagaggtcag cacgaaactg tgtcagcatg
       61 gctgtaaaca gcgcaggttg ctgtgcgtgg gtggcggcgc tcatgtcaaa ctcaccctgc
      121 atccagcaga cggccagcag aacgtttttg ggatttttct gcaatgccgc ttttgtgcgg
      181 gaaatcagat cctgatataa cggcttgccc accccccagc gtgccgaatc ctgactggcc
      241 cccgtggact cgctgaatgt cccctccgcg ccctgggtaa atgccgaacc accacgacag
      301 catggtacca gcaggatccc cgcgttattc gggatatacg ggagcagttt tttggcaata
      361 tgtaaaccct ggccgacaca gccgtactgc cctttgctca ggtcagccct cggatgattc
      421 agcgtactca tatcctgcac atcatgcaga cagtggtcag ccggaataat atcgttatat
      481 ctgcaggcag ccccgcccgg cgtcactgta ctgcggcgcg ccagctgttt aatgcgcgga
      541 tccggagcat cgtatgaatc cggcagcgga agcccttcac cgtaagccat ggcattggac
      601 tgcccggcca gtacgatgac gtagtaccaa tccggctcag atgaagggcc gacctgtggc
      661 tctccttcaa tagccaccgc ctgcatcagt gtgtacggcg taatggcaac cggtccgccg
      721 tatggctgcc agccctcttt cagtttgtgt gtcagctttt ccgcaaggtc tgacggcgac
      781 gccgccctga caacatcata atgtttaatc gacatcgaat ttctcccgtg tagaggaaca
      841 gagttaaaaa gccggaagcg gaatcaaatc acaggatgac catctgccag tggctggtcg
      901 taaaaaaagg ccgcgccatg cgcagccgaa aataaaggga taacgatgat agtttgagaa
      961 aaacagaaat aacacttttg tggcaaagca tggtgccggg tgcctcccgg tgaattcagt
     1021 atcagcacct gaatccgcga ttaccccata ttccttcttg ctgattgccc caccgcacag
     1081 ggggattcac catgcagaag tgtttttaat aaacagcaaa caaaaaaatc aagcattatg
     1141 caggctgttt ctttttatca ccggccacag caataccata atgccgcaga ccagcacccc
     1201 atccgccagc accgacatga ttctgctggt gaaatccacc atcaccacca gaaacagcag
     1261 gagtgcagcc acagtcaggc gcagttttac cgtcacaggt
//
LOCUS       DBFIFS010000443         1290 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.453, whole genome shotgun sequence.
ACCESSION   DBFIFS010000443 DBFIFS010000000
VERSION     DBFIFS010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.453"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..95)
                     /locus_tag="N7A07_004993"
     CDS             complement(<1..95)
                     /locus_tag="N7A07_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479449.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVL"
     gene            complement(247..618)
                     /locus_tag="N7A07_004994"
     CDS             complement(247..618)
                     /locus_tag="N7A07_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2479450.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(608..979)
                     /locus_tag="N7A07_004995"
     CDS             complement(608..979)
                     /locus_tag="N7A07_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2479451.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(992..>1290)
                     /locus_tag="N7A07_004996"
     CDS             complement(992..>1290)
                     /locus_tag="N7A07_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2479452.1"
                     /translation="SFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          374 a          354 c          233 g          329 t
ORIGIN      
        1 agcacagcca ggacaacaga caccgtcaac aatgaaaaag ccactacctt atgccaactg
       61 agagttgaca gtgacagtac taacacaatc cacataagca ctccttttat ttaatgatga
      121 agattgatta tcaatatttt caattcacca ggcaacattt tatctacctt ccacaataca
      181 tgaccatcaa aaaataaaca tgttaattat aaacacagaa aacataaccc tcatcactat
      241 atatccctac cgcatatcca tatctaaccg gacattcaga gccataagca ttccttcaat
      301 aataccttcc gctttataaa gccttttacc aataagccca tcagaacatc tatgcttacg
      361 tgcaagagcc ataaatgtca ttccacctac gtaataatcc accaacaaat cgtgcaaata
      421 ctgattattc ttgtttaatc gtgccataca accacaaatt atcatggcgt catcatcaga
      481 acacttagga cgtgatttca ctttagtcgg gattaatcct ttaaaaccag cagctatcga
      541 gggccatgtt acatcttcat gattatttgc tgcccatgcc ccccatcgct caagaaccat
      601 ctggatatca cgcgccatcg ttaccacctc tgatttcgta aatcttcacg cccagccgtc
      661 cacctggcac aggctgaccg cgcacaatat tgatttcatc aaactgctcg tcatcaatga
      721 gcacttccgc atgcgtcagc gcatccagcg gtgctttcag aatgttgtcc aggtcacggc
      781 ggcgcttatc cggtggttct gcaataattt ttattgccag ccgtccggac aggcttaatt
      841 tcagccgctg ctggcgaaca ataagcgcca cagcccggcg ataacgctca ccggcttttg
      901 atacaaaata tgtgctgcca cgacgtcgcc agtaggtgtt caccgtcggc gggtaaggca
      961 aaacaaactc tatacgcatc agtaacctct tttacccgag cacgccggtt gcaaaagcgt
     1021 gatcaagaaa acgaaaaatt aaatcaacct gggaaccatg cttttcttcg aacgccagcg
     1081 gatccgcatg aagctcgttg tgatgctccc gacacagcgg tagcgtgaaa atatcgtggg
     1141 attttgttcc cattccaccc tgaccatgac caatcagatg atgaggatcg tccgctggct
     1201 taccacaaca cgcacacggc tgtgtcttta cccagcgtgt gtatttctca tttacccagc
     1261 ggcgacgttt aggtcgtttc atgaaggatt
//
LOCUS       DBFIFS010000444         1289 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.454, whole genome shotgun sequence.
ACCESSION   DBFIFS010000444 DBFIFS010000000
VERSION     DBFIFS010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.454"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            443..634
                     /locus_tag="N7A07_004997"
     CDS             443..634
                     /locus_tag="N7A07_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479453.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(704..844)
                     /gene="ykgO"
                     /locus_tag="N7A07_004998"
     CDS             complement(704..844)
                     /gene="ykgO"
                     /locus_tag="N7A07_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU2479454.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(844..1107)
                     /gene="ykgM"
                     /locus_tag="N7A07_004999"
     CDS             complement(844..1107)
                     /gene="ykgM"
                     /locus_tag="N7A07_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU2479455.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          382 a          267 c          284 g          356 t
ORIGIN      
        1 agtcttaatg tgttgtgtaa tggactagta aaaaaacaag tctaaatgat aagaaataga
       61 atatgaaact atcatgagtc ataattaaag atgatttgcc aatcagtaag gtgttttaat
      121 ggattaacat gccgcacaaa atggttatgg gtgttcatcc caataagtga tgaaaaatta
      181 aacaggtgta agtacgtcat ctgtccggta agccccctaa gagccagatt aagtgaagtt
      241 aaacgaagac tgccagttgt caccggaacg atggcctctt tgccagggca ctgcattcag
      301 tcatctgtgc gcgttactct aactggcact cgaacgtcgg cgacggggtt accggtggat
      361 caggccgttc ctgtggcgta aacgttttta tgtcagtacc aacagattta ccacatttat
      421 catcttaaca gtttgagtgt aaatgcaggc tgcgtcgcaa agggaggaaa ctgagtggcg
      481 tgttcagtct aaaagagggt tgatgccagc atacaggggc gaagctggtc aacaagtcaa
      541 cattaatata atggaatatt cggagagaaa cgtcaggcag ttgaccttaa atgaacagga
      601 agatacatcc ccaggaaaat taatgctggt gtaataaata caccaacatt aatccggtct
      661 gattattaat cagacaaata tcgttaattc gtcgaatttt aaatcaacgt tttttcttac
      721 gaccctgaac ggccttaaag cgtggattag atttacaaat cacatatagc cgtccttttc
      781 gcttcacaat ctgacagtct ggatggcgtt cttttgcggt acgcagagag ttaaggactt
      841 tcatcacgcc ccctttttcg tgctaacaaa acgaccaaaa cgttgggtga atcgtgcaac
      901 atttccttct gatgccactg ttctcagctt ccctgtatag aacgggtgcg atttcgaaga
      961 gacatcaatt gttacgtacg gatacgttac gccatccagc tcaatctcac ggtctgtttt
     1021 gatagtcgag ccgattttaa agtactcatc aacactggtg tcgtggaaca ccacagtacg
     1081 atactcagga tggatattgg gcttcatcat ttttacctgt tatgttataa cataaccaca
     1141 aagatacatg ctcatccgga tcatttcaac acccaatccc tgtgaccatt agtggtatgt
     1201 ggaatttcct caacggggaa gtctggcatt gttaacagcc attggcgacg ctcccataaa
     1261 cgcctggatg agtgattggt aagcgtaca
//
LOCUS       DBFIFS010000445         1286 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.455, whole genome shotgun sequence.
ACCESSION   DBFIFS010000445 DBFIFS010000000
VERSION     DBFIFS010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.455"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            126..1172
                     /locus_tag="N7A07_005000"
     CDS             126..1172
                     /locus_tag="N7A07_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU2479456.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT          310 a          297 c          355 g          324 t
ORIGIN      
        1 ggtgattgtt ggtatgacat gaataaaagg tttttcgttt gactggaaaa ttatgttgca
       61 ggaacgagag gactgaggaa caaagcttat tccggcgctg tccgtccttg ctgtcactga
      121 cactgatgtc tgctttatta tcgcctttat ctcttcaggc agcggatgtc cggcgtagcg
      181 gagatgaagc atttatcatt cagcagcagc gtcaggaagc ccttgagcaa caactgacgc
      241 cttcagcccc tgatgttcgc ctttctgcac ctggctcttt tgcccataag attaattttc
      301 ctgttgaaac gccctgtttt cagattaaac agacggaact gaagggggct gatgcgttac
      361 cacactggct gcctttacaa aaaatcgcca acggggcggt cgggcattgc ctgggggcga
      421 aaggaattaa tctgctgatg agtacattgc agaaccgtct ggtcgatcat ggttatgtca
      481 ccacccgtgt tctggcacct tcgcaggatt taaaaagcgg tatcctccgg ctggttatta
      541 ttcccggtgt tgtgcgacat gtgcgtctga caccggacag tgatgactat attcagttgt
      601 attcctcatt cccggcacac gaaggttctc tgctggattt acgggacatt gagcaggggc
      661 tggatttagg taacagccgg atacagggac aacatactga gctgaatgca accagtggaa
      721 atctgtctac acagaatgcg caactgagtg ccgatacgct ttccgcccgg actgccgggc
      781 agttcagcag taatggcggt acgataaatg ccgacacact gcagatatcg gcacaaagcc
      841 tgtcaaatcg taaaggcagt ctgattcaga cgggaacagg ggatttttcg ctgagtctgc
      901 cgggaagcgt ggataaccgg gaagggctgc ttgcggcaaa tggcgcggtg cgtctggatg
      961 cactgagcct tgataatcgc aaggggaaag tgcaggcgga gcagtcaccc tcccttcaga
     1021 aatccccgcc cacgtttctg aaaccgtttg tggctggtgt ctgtgcggca ttgctggcgg
     1081 tcagcgtggc tattccggga tggcagtttc tgacacagcc atcaccggag gagcagcatt
     1141 ttacctgggg gaatggttgt aaaaagcagt gaaaacagta aatacagagt atcgtgatga
     1201 attagtgctc aggggctacc agtattcaat aaccagcgta tcggcatcgc attcctgtaa
     1261 ttctggtatt gactgttatg actaaa
//
LOCUS       DBFIFS010000446         1278 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.456, whole genome shotgun sequence.
ACCESSION   DBFIFS010000446 DBFIFS010000000
VERSION     DBFIFS010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.456"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..758
                     /locus_tag="N7A07_005001"
                     /pseudo
     CDS             1..758
                     /locus_tag="N7A07_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            771..1278
                     /locus_tag="N7A07_005002"
                     /pseudo
     CDS             771..1278
                     /locus_tag="N7A07_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          406 a          248 c          250 g          374 t
ORIGIN      
        1 tatcgaattc gacctcattt tccaaagatg gctattcctc caacaatact ggaattactg
       61 gcatagccgg tgaacatgat cagttaaatt atggtatata tgttaatcag caacaacaaa
      121 ataatgatac atcgcttggt acgaatttaa gctggagaac tcccatcgcc acaatagatg
      181 gcagctatag ccattctaaa aacgcctagc aaagtggtgg aagtattagt agtggattag
      241 ttgtctggcc cggtggtatt aatatcacta accagctatc cgatacattt gcaattctgg
      301 atgcgcctgg gttagaaggc gcgcatatta atggacaaaa atacaaccga acaaacagca
      361 aaggccaggt tgtttacgac ctgatgatac ctcatcgtga aaaccatctg gtacttgata
      421 cagcaaacag tgaaagtgaa acagaattgc agggcaatcg tcaaattatt gcgccttacc
      481 gtggagcagt ttcttatgtg cagtttacaa ctgaccaacg taagccctgg tatatacagg
      541 cactgcgtcc cgatggttcg ccattaactt ttggctatga cgtactggat ctccaggaaa
      601 acaatattgg agtcgttggc cagggtagtc gcctttttat tcgcgtagat gaaattccaa
      661 ctggcataaa agttgctctc aatgatgaac agaatttatt ctgtactatt acttttcaac
      721 acgttatcga tgaaaacaaa acatatatat gccagtaaaa ggagtttact atgaagtggc
      781 ttttattaat cacattgagt ctatattctt ttattgtgca aagtgctccc tgtggattga
      841 ccaatgtcgg ggaacaacgt ggaacatata tactcaaacc actgagtatg aaaggtaatc
      901 tcaccgccga aaaaattgaa aatactggga atatcattga gtgtgcagac ttgcaagaga
      961 cgaccaacgc cattaaagtt cattttttaa cacagaacgc tttaaggttt aaagtcggaa
     1021 gtcgtcatta tattattaat ttcccctttg aatctgacgg taaaaaaaat agcgcttcca
     1081 gggaagttat cttataattt agaagaaatg ctatctgcga taaatttcac cattccatac
     1141 tctatcgcat ctgtatcaga tagttccaac accgcagata tttttccagg aactcctttt
     1201 cctttgttgt tcgaaattga agtgagttca tgtacaggga ataatactaa ttgtaatagc
     1261 gtccgcttta attttaat
//
LOCUS       DBFIFS010000447         1277 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.457, whole genome shotgun sequence.
ACCESSION   DBFIFS010000447 DBFIFS010000000
VERSION     DBFIFS010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.457"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..81
                     /locus_tag="N7A07_005003"
     tRNA            6..81
                     /locus_tag="N7A07_005003"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="N7A07_005004"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="N7A07_005004"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="N7A07_005005"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="N7A07_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU2479457.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT          324 a          334 c          304 g          315 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctg
//
LOCUS       DBFIFS010000448         1271 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.458, whole genome shotgun sequence.
ACCESSION   DBFIFS010000448 DBFIFS010000000
VERSION     DBFIFS010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.458"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..716)
                     /locus_tag="N7A07_005006"
     CDS             complement(<1..716)
                     /locus_tag="N7A07_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HGU2479458.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVS"
     gene            complement(713..1270)
                     /locus_tag="N7A07_005007"
     CDS             complement(713..1270)
                     /locus_tag="N7A07_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2479459.1"
                     /translation="MEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHELFADAL
                     PFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQSLIQTSL
                     YDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTIAHKYGY
                     SSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          381 a          276 c          208 g          406 t
ORIGIN      
        1 gatacgtttt ttagttgttg acgacttgaa atttcagaaa gtacgttcac ccctgtgctt
       61 tgcttaatgt tattatttaa caacgtcgat agtacttttg ccatgccagg aataactatc
      121 ttttcgccac gtaactcata agtccggtca ttaacgaatg tgttgaccag atgaattatt
      181 cctactttat ttcgaccgat ctcaatgcca tcgctgtttt gctccattaa tttcgcagca
      241 ttaacgacca ggtcaacata aaccggtggc cccgatacat agaaagtacc attgccgtcc
      301 cctttaattt cataacgaga gttatacaga cccgattttt ttaagaaatt attaaactca
      361 ttcgtcgata cctttctcag attaatcaat gcattacgca tctctgatga gtcatagata
      421 taaatagctt tgccatcatc ataccaaata agccccatct gtgccgacag gcgttcaatc
      481 aaggcatagg gttctgttaa atcaaaattt ccgctgatct gtttcttcat ggcaagctta
      541 ctaactattg taggcttacc cgcatatgat gaaaccgttt caaaaaaact acgtagattt
      601 tctttatttg ctacgtatcc attcgctgct gcggcatatg caccaggagt caataatcca
      661 ttaaaaatgc ataagactga aattaatata atagtaatgc gtaattttat tttcataact
      721 ttttacatag ttctctcggt gtttttccta accggctttt tacttcggct gaaaagtgtg
      781 atgaggacga gtaaccatat ttatgcgcaa tagttgtcat atcactgttt ccttcaatga
      841 tttccaacac ggctctggca actctccagc cacataattc cgttttaact ttaccaccca
      901 atgcataact acataaacgg cgaaaatgtg aatacgatac gccgtaaagc tcaccaagat
      961 catagagtga agtctgtatg agcgattgcg ataataaata acgaaccagt ttatagcatt
     1021 ctgtttttct tagatgctga aacaggattt cattaatatt atcgctgctc agataagtgg
     1081 ctaaaaacca ggcttcactt gcaactttac tgaatagcac aacttctgat gcaaagggca
     1141 aggcatcagc aaacaattca tggtgttccc tttccacact ccccgttgat tcatctataa
     1201 aagctaataa tttactcagg atatcaatgc gaagtggatg ataaattact ttccctgcag
     1261 ttacctcaat t
//
LOCUS       DBFIFS010000449         1265 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.459, whole genome shotgun sequence.
ACCESSION   DBFIFS010000449 DBFIFS010000000
VERSION     DBFIFS010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.459"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..888
                     /locus_tag="N7A07_005008"
     CDS             <1..888
                     /locus_tag="N7A07_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU2479460.1"
                     /translation="VFGGAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIF
                     MWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVS
                     KLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIM
                     DIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERA
                     KALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAK
                     ETGRNKVVVRDVVNFCESP"
     gene            986..>1265
                     /locus_tag="N7A07_005009"
     CDS             986..>1265
                     /locus_tag="N7A07_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479461.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSI"
BASE COUNT          369 a          225 c          285 g          386 t
ORIGIN      
        1 gtttttgggg gggcgatagt tgctcacata ctttctagtc attatgagag ctacaattta
       61 cacattgccg agttaaccaa tgaaaatggt caggtggttt ggaaagcctc atatgttacc
      121 ataatgattt tcatgtggtt aaccttgctt tcggttaatc tatactttaa tggtttacgc
      181 tatgatatct ggaatggagt gacggtgatt gctttttgtg ccgtactgta caatatctct
      241 ctgttattta tgagtaggta tagcgtgtcc acttggtata ttagtcgcac tattgaagta
      301 gtcagtaagt taaccgttat ggtaatattt atgtgccata tattcagtgc gctacgagta
      361 acaaagaaca ttgcacatcg cgatccctta accaatatat ttaacagaaa ttattttttt
      421 aatgaactga cagttcaatc agcatcagcc aaaaaaacgc cttattgcgt catgattatg
      481 gatatcgacc acttcaaaaa agtcaacgac acctgggggc atccggttgg cgatcaggtg
      541 ataaaaacag tggtgaatat cattgggaaa agcatacgac cagatgacct tttagcgcgc
      601 gtcggcggcg aagagtttgg cgtcttgctg acggacatcg atactgaacg tgcgaaagct
      661 ttagcggaaa ggattcggga aaatgttgag cgtttaactg gcgataatcc tgaatacgct
      721 ataccacaaa aagtgacgat tagtattggc gctgttgtga ctcagggaaa tgcgttaaac
      781 ccgaatgaga tctatcgact ggctgataac gcactatacg aggcgaaaga gaccgggcgt
      841 aataaggtgg ttgtgaggga tgtggtgaat ttttgtgagt caccataaag cggcatttta
      901 gcgggtgatt ttggtaagcg ctaaatagct attcgcagac atgcgtaaca attttcagaa
      961 atatcatagt actatatgat cttaaatgct tatcattgat cagtctgcta tcacccgctt
     1021 ttactggcaa ttgtactata atttcttagg ttgcatcacc ctgatgcgtg gggaaacatg
     1081 tatggtattc aacaaaaaaa tgtttgtact cattataata ccaggcattc tgggggtgtt
     1141 actgtcgttt gctatgtcag tatttcaaat gaatcgagat acgacaatta ccgccggtat
     1201 tctgttaaaa cagcttgata atgttacgca aattgtcaaa catacgacaa agcttacttc
     1261 tattc
//
LOCUS       DBFIFS010000450         1262 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.460, whole genome shotgun sequence.
ACCESSION   DBFIFS010000450 DBFIFS010000000
VERSION     DBFIFS010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.460"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..196
                     /locus_tag="N7A07_005010"
     CDS             <1..196
                     /locus_tag="N7A07_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU2479462.1"
                     /translation="ARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDH
                     QAVQAWADSLSTDNPLPVPR"
     gene            196..471
                     /locus_tag="N7A07_005011"
     CDS             196..471
                     /locus_tag="N7A07_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2479463.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            566..772
                     /locus_tag="N7A07_005012"
     CDS             566..772
                     /locus_tag="N7A07_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479464.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(872..1087)
                     /locus_tag="N7A07_005013"
                     /pseudo
     CDS             complement(872..1087)
                     /locus_tag="N7A07_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1131..>1262)
                     /locus_tag="N7A07_005014"
     CDS             complement(1131..>1262)
                     /locus_tag="N7A07_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2479465.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          343 a          289 c          311 g          319 t
ORIGIN      
        1 cgccagactg gaacgctccc ggggttgggt tatcaaacag gcgctttctg catggcttgc
       61 ccaggaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga catccggaca
      121 ggttattgac catcaggctg tacaggcctg ggcggacagc ctcagtactg acaatccgtt
      181 accggtgcca cgctgatgga actgaagtgg agcagtaaag cgctttctga tttggcgcgg
      241 ttatatgatt ttctggtgct agccagtaaa cctgctgccg ccagaacggt acagtccctg
      301 acacaggcac cggtcattct gttaactcat ccacgtatgg gggaacagtt atttcagttt
      361 gaacccaggg aggtcagacg gatttttgct ggcgagtacg aaatccgtta cgaacttaat
      421 ggccagacta tttatgtatt gcgcctgtgg cacacacgag aaaacaggta gattgtgtgt
      481 ggggacaact taacatagcc tgcagatatt aattctggaa cattattatc aggccagata
      541 tttaaataat cttgtaggag ataaaatgaa ttacgatgaa ataacgaaaa ttaccgctga
      601 acgtattagc gactacatga ctgaagcagt taatacagac tctatagccg ttgcggaaat
      661 gtttcataac gcagcatggg gagtgcgaac attgtggttt gaactcgtca caaaaataga
      721 tatagatata cataaaaaga acagatatgc gagttatgat cttgatcgat aggaatttaa
      781 atcccaaaaa gattaaaaaa acacccaaaa acggatgttt attcaacact gcctgcgtgc
      841 catgagtgca ccgccgtcaa ttaaatggat ctatttcatc ctgaccagca tagtgctcca
      901 gacggctcat cggcaccggc ggttttttca ggtaagagcc gaagtacacc gccacatgtg
      961 tggcgttatc catcacccgg gatacgttga cattccagcc ccgccggtaa tgactgtcca
     1021 ggaagcgatt ccagtcccgt ttactgcttc cttctgctgc cagcgcatcc ggtatcacca
     1081 ggtccggtga ggtgtactgg caatagcgga cactaccatt tgttcttttt ttaagcagcc
     1141 atctgatgat atttttccct gaaggctgcc ggggagatat tccccagacg agagtgacga
     1201 cgctgacgat tgtagaaaat ctcaatgtat tcccgtatta ctgagatggc ttcatcccgg
     1261 tt
//
LOCUS       DBFIFS010000451         1252 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.461, whole genome shotgun sequence.
ACCESSION   DBFIFS010000451 DBFIFS010000000
VERSION     DBFIFS010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.461"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            83..430
                     /gene="tnpB"
                     /locus_tag="N7A07_005015"
     CDS             83..430
                     /gene="tnpB"
                     /locus_tag="N7A07_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2479466.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            480..>1252
                     /locus_tag="N7A07_005016"
     CDS             480..>1252
                     /locus_tag="N7A07_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGU2479467.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPG"
BASE COUNT          268 a          363 c          372 g          249 t
ORIGIN      
        1 taacgttccg gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc
       61 tgctgataca ggaactgaag cgatgatccc gttaccttcc gggaccaaaa tttggctggt
      121 tgccggtatc accgatatga gaaatggctt caacggcctg gctgcgaaag tacaaacggc
      181 gctgaaagac gatcccatgt ccggccatgt tttcattttc cggggccgca gcggcagtca
      241 ggttaaactg ctgtggtcca ccggtgacgg actgtgcctc ctgaccaaac ggctggagcg
      301 tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg ttccttacgc aggcgcagct
      361 ggcgatgctg ctggaaggta tcgactggcg acagcctaag cggctgctga cctccctgac
      421 catgctgtaa atctctttat cctggttgtc acagaataag cccggtaaaa tacgggctta
      481 tgaacgacat ctcttctgac gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag
      541 cgctgatcca cgccctgcag gaaaagctga gcaaccggga gcgcgaaata gaccatctgc
      601 aggcgcagct ggataaactc cgccggatga acttcggcag tcgttccgaa aaagtctccc
      661 gccgtatcgc acaaatggaa gccgatctga accggcttca gaaagagagc gatacgctga
      721 ctggtagggt gtatgacccg gcagtacagc gtccgttgcg tcagacccgc acccgtaagc
      781 cgttccctga atcactaccc cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc
      841 cgaactgcgg cggttcactg agctatctgg gcgaggatac cgccgaacag ctggagttga
      901 tgcgtagcgc cttccgggtt atccggacgg tacgggaaaa acatgcctgt actcagtgcg
      961 atgccatcgt gcaggcacct gcaccttcgc ggcccatcga gcggggtatc gccggaccgg
     1021 ggctgctggc ccgcgtgctg acctcgaagt atgcagagca caccccgctg tatcgccagt
     1081 cagaaatata cggccggcaa ggtgtggagc tgaggcgttc actgctgtcg ggctgggtgg
     1141 atgcatgctg ccggctgctg tctccgctgg aagaggcgct tcatggctat gtcatgactg
     1201 acggcaaact ccatgccgat gataccccgg tccaggtact gctgccgggt aa
//
LOCUS       DBFIFS010000452         1244 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.462, whole genome shotgun sequence.
ACCESSION   DBFIFS010000452 DBFIFS010000000
VERSION     DBFIFS010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.462"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..1153
                     /gene="nleA"
                     /locus_tag="N7A07_005017"
     CDS             <1..1153
                     /gene="nleA"
                     /locus_tag="N7A07_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HGU2479468.1"
                     /translation="ETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          395 a          244 c          264 g          341 t
ORIGIN      
        1 tgaaacaaca gctctactga gcttgataaa agataaaggt ctgctctcag ggctagacga
       61 atatatagct cctcaccttg aagaaggatc cataggaaaa aaaacattgg atatgtttgg
      121 tttattcaat gttacccaaa tggcattaga gatacctagt tccgtttcag gcatctctgg
      181 taaatatggt gtccagctaa acattgtaaa accagatatt catcctacat caggtaatta
      241 ttttttacag atattccctc tgcatgatga aataggtttt aattttaaag accttcctgg
      301 cccgttaaaa aatgcattaa gcaacagtaa tatatcaacc actgcagtgt cgactattgc
      361 atcgactgga acatcagcca ctacttcgac ggtaaccacc gagccaaaag acccaatacc
      421 atggtttgga ttaacagctc aagtggttcg taatcatggt gtagaacttc ctatagtcaa
      481 aactgaaaat ggatggaagc ttgttggaga aacaccactt actcctgatg ggccgaaagc
      541 aaattacacg gaggagtggg ttatcagacc gggagaagca gattttaaat atggtgcatc
      601 tccattacag gcaactctag ggctggagtt tggcgcacat ttcaagtggg atttagataa
      661 ccctaatact aaatatgccg ttcttaccaa tgctgccgca aatgcgcttg gtgctttagg
      721 gggatttgca gtatccagat ttgctagtac agatccaatg ttaagtcctc atatcggtgc
      781 aatggttggg caagcagcag ggcatgctat acagtataat acccctggat taaagccaga
      841 cactatttta tggtgggctg gtgcgacact gggggctgcc gatttaaaca aggccgagtt
      901 tgaagtagct agattcactg actatcctcg tatatggtgg cacgcaagag aaggagctat
      961 tttccccaat aaagcagata ttgaacatgc cacaggtgct gatatacgcg caatggaaga
     1021 aggtatccct gttggacagc ggcatccaaa tccagaggat gtggtaatcg atatcgaaag
     1081 caatggctta ccacatcata atccatcaaa tcatgttgat atctttgata taatccaaga
     1141 aacaagagtc taagctttaa cttgtttatt ttaaaataat agctgaaatg gatggcagct
     1201 attattaata aggattaata tattttttct aaaatacccc acaa
//
LOCUS       DBFIFS010000453         1238 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.463, whole genome shotgun sequence.
ACCESSION   DBFIFS010000453 DBFIFS010000000
VERSION     DBFIFS010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.463"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..441
                     /locus_tag="N7A07_005018"
     CDS             <1..441
                     /locus_tag="N7A07_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2479469.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="N7A07_005019"
     CDS             597..779
                     /locus_tag="N7A07_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479470.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="N7A07_005020"
     CDS             792..923
                     /locus_tag="N7A07_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479471.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..1170
                     /locus_tag="N7A07_005021"
                     /pseudo
     CDS             931..1170
                     /locus_tag="N7A07_005021"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1151..>1238
                     /locus_tag="N7A07_005022"
     CDS             1151..>1238
                     /locus_tag="N7A07_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479472.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAP"
BASE COUNT          307 a          263 c          366 g          302 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc cgctggtcgt
     1201 aagcgcctgc gcatcaacgc caactgtcca ggctcctt
//
LOCUS       DBFIFS010000454         1231 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.464, whole genome shotgun sequence.
ACCESSION   DBFIFS010000454 DBFIFS010000000
VERSION     DBFIFS010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.464"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..376)
                     /locus_tag="N7A07_005023"
     CDS             complement(<1..376)
                     /locus_tag="N7A07_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2479473.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAAC"
     gene            620..943
                     /locus_tag="N7A07_005024"
     CDS             620..943
                     /locus_tag="N7A07_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU2479474.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          363 a          333 c          246 g          289 t
ORIGIN      
        1 tgcatgctgc accacccggt gtcaccgtac tgcgacgcgc cagttgctta atacgcggct
       61 ccggacggtc atatgtctcc ggcagcggaa gaccttcacc atacgacatg ccgtttgact
      121 gccctgccag aaccacaaca aagtaatact ccggggctct ggtggcactg acagccaccg
      181 ccttaccctc attaccggtc accgccactg gtgtggtgac atcaccttcc gccgcaatgg
      241 cctgcatcag ggtgtaaggc gtgatggcca ccggactgcc aaatggctgc cagccctcct
      301 tcagtttttg tgtcagtcgt tcagcaaggt ctgacggcga tgccgccctg accacatcgt
      361 agtgtttaaa tgtcataacc tctcctgccg ccagcaaagt aattatccgg aacacacaat
      421 catgtaacac ataaaaaaca cgctataaaa aaaccatgtc tcttatataa caaaaaaacg
      481 ccgacaattt aaaatgccct cttattttaa acaaagaata aacgacacca gaaaaacaac
      541 tcagcctgac agattgcttt caaaacatca cctataattt atggcatcat ttaaaacctt
      601 aagaaaataa ggaaagcaaa tgaactggat agtgattgac acggtaatcc agccagcatg
      661 tggcatatca ttttcagcca tatggtgtaa cataaaatta atattatggt atcagtcaga
      721 taccttcctc cctccgggct gtatttttac accaacccgg acaggcgtaa tactcaataa
      781 taaagagctc cccgtcacca tttacaacgt ggccccattt aataaaaacg tctggaattt
      841 aattaaaaac agccaggagt gccctgcaaa cacaggaaat atcacagata agtgttttaa
      901 tcatcgctgc attctggaaa tatgcccata cgggcgaaaa taacagaatc attcagataa
      961 aaagcccctc cggagagggg ctgacactgc gtatctgtat catcatgtac atggtgccgg
     1021 gtgcctcccg gtgagttcag tccggtgtcc ctgaacccgc gtatctcgct ccaggttgtc
     1081 gtcagagatg acaccttata caccagtcgc ccctccgcac agggggattc accatgcgaa
     1141 tttttttaac gaatgctcag tctgacaggc aactgtcaac tgactgaatt gtgacacaga
     1201 ttacacttgt tacccacata ccacgaatca g
//
LOCUS       DBFIFS010000455         1206 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.465, whole genome shotgun sequence.
ACCESSION   DBFIFS010000455 DBFIFS010000000
VERSION     DBFIFS010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.465"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..725)
                     /locus_tag="N7A07_005025"
     CDS             complement(<1..725)
                     /locus_tag="N7A07_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU2479475.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGI"
     gene            complement(773..982)
                     /locus_tag="N7A07_005026"
     CDS             complement(773..982)
                     /locus_tag="N7A07_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU2479476.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(1078..>1206)
                     /locus_tag="N7A07_005027"
     CDS             complement(1078..>1206)
                     /locus_tag="N7A07_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2479477.1"
                     /translation="DLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          254 a          404 c          292 g          256 t
ORIGIN      
        1 aatatcccca gctggctgat taacgcgggg accactttat ccgctgtcag ttttccgtca
       61 tccgccattg ccttcagatc tttacgggcc acgcccatac ccgcagccag tgcacgtacg
      121 atccggtcac cactttcatt gaccgaatta aactcctcac cacgcaatac accctgcgcc
      181 agcgcctgac tgaactgggt gatcaccgag cccgcctctg ccgtactggc accggagatt
      241 ttcagccctg tcgaaatggc ctccgtcacc ttcagcacat catcagcact gtaaccatat
      301 tcacgcatcg aggcagccga acgggcaaac agggccgcat tatccgaaaa tgcggtgcct
      361 gtccgctggc tgatatccat cagcactttc tgtgatgacg caaattcatc ggatgactgt
      421 gatgcctgtt tcagacgggc attcacggag ctccattcat ccgccagcga aatcaggtgt
      481 ccggtggcaa aggcaccggc aaacgcaccg gtcattccga cagccgaagc gcggatttcc
      541 gtcaactggc tgtgcagctc agccagagcc cggcgctgct ccctggctgc cgcagcagcc
      601 tgacgtccgc cattctgcag ggtccggtaa tattcactgc ccatacggga cgcccgctgg
      661 atctccgact ggaatgactg tgaatttgcc gaaattttga taatcagttc acgtaacgtc
      721 gccattcacc tttctccggg cgtaaaaaaa ccgcctcagc ggttctcatc attcatgact
      781 gtgctgcaag gctcagcgcg tcttccagcg ccgcaaacgg atccacctcc ggcttatcct
      841 catcctcgcc ccagcagagc atggcgtcct tcagtgcaac attcatcccc tgtgccccga
      901 aaaccgcttt cacgatctgt gcattacgga tatccccgcg ctcatcaccc agcggggata
      961 tcctgtcgaa ctccatccac atcatcgcct cgctcgcact caggctgtgg cgcagttcgg
     1021 ataaggtgcg ccccagacgg agcgcaagtc gcatcagaaa gcgaatttcc gggcgggcta
     1081 cttttttctg gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc
     1141 atgtaccgga ccatagacgg ccagcacctg ctcacggtcg tccggagtga acacccgctg
     1201 caggtc
//
LOCUS       DBFIFS010000456         1204 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.466, whole genome shotgun sequence.
ACCESSION   DBFIFS010000456 DBFIFS010000000
VERSION     DBFIFS010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.466"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(1..270)
                     /locus_tag="N7A07_005028"
                     /pseudo
     CDS             complement(1..270)
                     /locus_tag="N7A07_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            364..627
                     /locus_tag="N7A07_005029"
                     /pseudo
     CDS             364..627
                     /locus_tag="N7A07_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            667..787
                     /locus_tag="N7A07_005030"
                     /pseudo
     CDS             667..787
                     /locus_tag="N7A07_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            784..936
                     /locus_tag="N7A07_005031"
                     /pseudo
     CDS             784..936
                     /locus_tag="N7A07_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          355 a          268 c          277 g          304 t
ORIGIN      
        1 tggttcgggc cactggcgta aaaatcctgc tccagcagat tttctgacac aggctggctg
       61 tgtgcgcggt aacagaccgg actgaaattc caggaagctt ttgccctcag tccgtgacgg
      121 cgcaggcttt ccgtcacggt ttttacttta aaatggtaac cctgggcacg cagttcatcc
      181 atcaggcgtt gtgcatgacc gggtaaaagc aacgaggaca acgctgtcgc agtgctgacg
      241 gaactgccga cgtgtactta tccttatccg ccagtgatat aagacggtaa ttcaccattt
      301 ggattgtccg ctccacccaa catgttgttt ccttgaggtt ctcacaccag aaaggacatc
      361 agcatgctga gcagagagga cttttacatg ataaagcaaa tgcgccagca aggtgcgtat
      421 attgttgata ttgcgactca gattggttgc tctgaacgaa cggtcagacg ttacctgaaa
      481 tacccagaac ctccggccag aaaaacacgc cacaaaatgg ccgaaatgga ctatatcgac
      541 atgcgtctgg ctgagaatgt ctggaacggc gaagttatcc tggcggaaat caaagcgatg
      601 ggttataccg gtggccgttc catgttgctt cgccagttca ggaagacacc ggagcagcgc
      661 tttacgcagg aacacagtct gttcttccgg ttgctgaacc gtcgatatga aaaagcgagc
      721 attgtgctga cgtccaataa agggttcgcc gactggggga gagatgttcg gtgataacgt
      781 gctggcaaaa tattcaccca acgcattgag cgtaataatc tgacgctacg cacccgcatt
      841 aagcggttga ctcgtaaaac catctgcttc tcacgctcag ttgagatcca cgaaaaagtg
      901 atcggagcgt ttatcgaaaa acacatgttc tactaattgg atgcaccacc gaatacatta
      961 atttatataa caacatcaaa atacatttta ggttgtcgct aatcaaaaat tattaagtgt
     1021 tacataatta gagggtatag atatgattca tcctggctct tctttagata aagcaattaa
     1081 caatacaaga gttaaaaatg ttagtactga tgttaaacat ggccaaattc aagagagaaa
     1141 aaggaacttt atctataaaa aaaatgatga tatctcatcc agatttaaac tttacagttc
     1201 gctc
//
LOCUS       DBFIFS010000457         1203 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.467, whole genome shotgun sequence.
ACCESSION   DBFIFS010000457 DBFIFS010000000
VERSION     DBFIFS010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.467"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            40..501
                     /locus_tag="N7A07_005032"
     CDS             40..501
                     /locus_tag="N7A07_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU2479478.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKVVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            547..756
                     /gene="hha"
                     /locus_tag="N7A07_005033"
     CDS             547..756
                     /gene="hha"
                     /locus_tag="N7A07_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU2479479.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            794..1132
                     /locus_tag="N7A07_005034"
     CDS             794..1132
                     /locus_tag="N7A07_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479480.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
BASE COUNT          351 a          248 c          304 g          300 t
ORIGIN      
        1 ctgaaataca ggtgctattt atctgatacg gagaataaca tgaggaaata tattccactg
       61 gtattattta tcttttcatg gccggtattg tgtgcagata ttcatggacg ggttgttcgt
      121 gtactggacg gtgataccat cgaggtcatg gactcacgga aggtggtgcg gatcaggctg
      181 gtaaatattg atgcgccgga aaagaaacag gattatggcc ggtggtcgac agacatgatg
      241 aaatcactgg ttgcaggaaa aacggttacc gtcacatatt ttcagcgaga tcgttatggt
      301 cgtatgctcg ggcaggttta tgcaccagac ggaatgaatg ttaatcagtt tatggtccgg
      361 gccggggccg cctgggtata tgagcagtac aacacagatc ccgtgctgcc tgtccttcag
      421 aacgaagcca gacaacaaaa acgtgggctc tggtcagatg ctgatccggt tcccccctgg
      481 atatggagac accgtaaata aagcgatggc aatcttatca ttcatctgtt ctcagcggga
      541 ataaaaatgg aaaaaacaaa acaggaatgg ctgtatcaat tacggcgatg ttcatcagtc
      601 aatacgctgg aaaaaattat tcataaaaac agagacagtt tatcgaccag tgaacgtgaa
      661 tcatttaatt cagcagcaga ccaccggtta gcagaattaa tcaccggaaa gttatatgac
      721 aggataccaa aagaaatatg gaaatatgta aggtaatagt tcagcataat taattaccag
      781 gagcagataa acaatgaagc tgattatttt tattctcatt gttctgatta ttgcagcgtt
      841 acttatccgg attatcctca gaagtgtaaa tcagcattct cctttgctga tgcaattgca
      901 tgcggcaggt atacggaccg gtgatgcaga gcgaatactg tccggcggtg aatatgatgc
      961 cagccattta cgacctgaac gcagacgccg ggatattctt ctggaagagg ttctgaggca
     1021 ggctggtatt ccgttgctca gaagccacga tgccagaaaa ctgctgcaga tgaccggaga
     1081 atggctgaat acaacagggg ctgctcagca gtcaccggaa catcgtagct gacgccttcg
     1141 cgttgctcag ttgtccaacc ccggaaacgg gaaaaagcaa gttttccccg ctcccggcgt
     1201 ttc
//
LOCUS       DBFIFS010000458         1188 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.468, whole genome shotgun sequence.
ACCESSION   DBFIFS010000458 DBFIFS010000000
VERSION     DBFIFS010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.468"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..673
                     /locus_tag="N7A07_005035"
                     /pseudo
     CDS             1..673
                     /locus_tag="N7A07_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
     gene            633..>1188
                     /locus_tag="N7A07_005036"
     CDS             633..>1188
                     /locus_tag="N7A07_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HGU2479481.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIGFSKTCYRTIVDIEV"
BASE COUNT          431 a          210 c          244 g          303 t
ORIGIN      
        1 gaagtctatt gacaacaaat cgtctctcta ggttgacagg aatccatctc tttctttgca
       61 aacaaaagga gccgagcaat tttctacaaa gaaacttctc aatatgactt cacgagatca
      121 gggtattaat tctgaactat ctaatcgaac gatacaattt aaggaaaaaa tccataatgg
      181 tatccataca gaatacatca cagatcaaaa acacagtaat aataaagatc gtgaaaaaaa
      241 ataccgcgat ggagacaaaa taaatggacc gcaggcccat tcattagaca ttactaacga
      301 aagaaggttt gctgataaca gaaccatgtt tactcaacat atcgaaaagc aaaggaatgt
      361 taatacgctc aatcagaatg atataaataa ttctgctaat aatgccaatg tgcgcgaaaa
      421 tgaattgaca tatcagtttc agcgttgggg acaaaatcat acggtccgga ttctggaaag
      481 tagtgaaggg atacgactaa aaccatcaga cacacttgtc tccgacagac tccatgaagc
      541 ccaacacaat gatgttacag cacaacgttg ggtattgact gagcaagatg aacgacaagg
      601 acaacgacat cagcctcatg aggagcaaga aaatgaaggc aaattcgaaa acgatcagaa
      661 ggatgaaagt taatgttcgg tctgaggaaa gtaaatcgaa acactctacg tttgaaacca
      721 cttttcaaaa ttggaaagaa aatggcgaag atgttgcatt attaatgccc gaatttagtg
      781 ctaaatggct accgatagca gaagagagtg gcagctggtc tggatgggtg ttgcttcgag
      841 aaatatttcc cttaatttct gccgagctcg ctgggatggc tttaatgcca gaaacggaaa
      901 ggttaatagg ggaatggctc agtttatcca gttccccgtt aaatctaaag tatcctgaac
      961 taaaatataa tcgtttgtgc gtaggtaaag tatttgatgg agtattgagt ccagctcaac
     1021 cactgataag aatatggaca ggggaattga atctttggtt agataaagtc acagtctgcc
     1081 aatacgaaaa cgctccaact ttagacaaaa aatcattata ttggcccatt cattttgtaa
     1141 ttggatttag taaaacatgt tatagaacta ttgttgatat tgaagtcg
//
LOCUS       DBFIFS010000459         1180 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.469, whole genome shotgun sequence.
ACCESSION   DBFIFS010000459 DBFIFS010000000
VERSION     DBFIFS010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.469"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..253
                     /locus_tag="N7A07_005037"
     CDS             <1..253
                     /locus_tag="N7A07_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2479482.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="N7A07_005038"
     CDS             268..801
                     /locus_tag="N7A07_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2479483.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="N7A07_005039"
     CDS             798..>1180
                     /locus_tag="N7A07_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2479484.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DBFIFS010000460         1178 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.470, whole genome shotgun sequence.
ACCESSION   DBFIFS010000460 DBFIFS010000000
VERSION     DBFIFS010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.470"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(33..920)
                     /gene="rhtA"
                     /locus_tag="N7A07_005040"
     CDS             complement(33..920)
                     /gene="rhtA"
                     /locus_tag="N7A07_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU2479485.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          349 a          300 c          284 g          245 t
ORIGIN      
        1 atggttatgc ataaccatgc agaaatgtaa atttaattaa tgtctaattc ttttattttg
       61 ctctctttgc gtactgtcag cgtagaaccc attgaagcgg cgataattgc gccgagcgcc
      121 agcagctgta tgggtgtcag tgtttctccg aggaaaatca tcccggaaac ggcagccagc
      181 gccggttcca tgctcatcag cgtaccaaat gtccgtgttg gcaaacgggt gagggcaatc
      241 atttccagcg aataaggcag agcggtcgag agaatagcga ccgccagacc caatggaata
      301 accgaccagt gccagagtgc ttcaccagcc tgaagcgctc caattggcac gaaaattaac
      361 gctgcaatca acgaaccaat tgccaccgtc gcagggccat gttccgctcc tgcgcgttgc
      421 ccacttaaaa tgtaaatagc ccaacaagcc ccggccccca gtgccagcgc acagccggtt
      481 aaatcgacat gggaaacgtc ttgccccagc ggtagcagga accacagacc aagaaccgcc
      541 agcacaaccc agacgaaatc taccgggcga cgagaagaga acagcgccac cgccagtggt
      601 ccggtgaact ccagcgccac cgcaataccc agcggtaccg tctgaataga gagataaaaa
      661 agataattca tcccacccag cgaaacgccg taaaacaaca gcggtaaccg ttgctctttg
      721 gcaaagcgca gtcgccatgg cttaaagaac gcgatgagga tcagcgttcc taatgccagg
      781 cgcagcgcag tgacacccgg tgcgcccacc agaggaaaaa gtgacttagc taacgaagct
      841 ccaccttgaa tagacgccat ggcaacgagc aatatgacta ttggtaacca gaccggcatt
      901 ttacgtaatg aaccaggcat cctttctccc acaaatatct agacttaagt aaagcgtgga
      961 gtgtactgga tatacccaat gctggttgag catttgttga aaaaattttc ccccgttttg
     1021 actaaaatgc gccaggattg atggaatcat tagtctggtg attaggaata atctggatga
     1081 atgacaggga aaacatgcgt aatacttacg cagttctctg aaaaagtgat ttaaatttag
     1141 atggatagcg gtgtatggaa acgttctgtt acatgaaa
//
LOCUS       DBFIFS010000461         1170 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.471, whole genome shotgun sequence.
ACCESSION   DBFIFS010000461 DBFIFS010000000
VERSION     DBFIFS010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.471"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(359..586)
                     /locus_tag="N7A07_005041"
     CDS             complement(359..586)
                     /locus_tag="N7A07_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2479486.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            628..993
                     /locus_tag="N7A07_005042"
     CDS             628..993
                     /locus_tag="N7A07_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2479487.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          274 a          257 c          315 g          324 t
ORIGIN      
        1 tgctgatgcg ccgtatcgtc gccgtattcc tgcattaaca gagaccgcag cccgacaggg
       61 agactcctct gcgagagtgt gcggggataa tcaaaaacga tacacaccgg ggtttaccgc
      121 gtaaacggag cgcggcgttc tcccctcatg gtcgcccgtc cggtgcgatg gtggaagaaa
      181 ctggaatctg ttcaataaaa aaactgccgt gttggagtca cagcagtaat gtactgattg
      241 ggtagaagat tattattgtt atgctttatt tttattctat atggctgatt atttcaattc
      301 ggaattaata cagctaatgt ctgtgagttt ttataaattc agcaatataa agaaatagtt
      361 atatgaacag ccatcgcaga gcatactgtg tatcattctt ttttatagtc aactgacggg
      421 catattttat gtctgctgcc agctcccggc ggcaagattc aatgacccac gcagaaaaat
      481 tttctgaacc tttctggtca agagcgatgt taatttgttc aatcatctgg tttggaaatc
      541 ggatgttgcg ggttgttgtt ctgcggggcc ggtttttcga tgacattttc tttcctctgg
      601 tgacaagcta tatggcgagg attttacatg gctgtgcttc gtacgttacc gggcagaatc
      661 aaaactctga acacccggcg ggtgaatatt ctgaagggtg aacagcgtcg tgtcagtggc
      721 agtgcacgtg tttccctcaa gcgtcatatc tggctcaggg atgccgggca gtgctgtctc
      781 tgtggtcgtg tggttgacct ctgtgacagt gaactcgatc accgaattgc acttcagttc
      841 ggtggtggta atgaggagac gaatctctgg acgctctgta ccgaatgcca tcgacaaaag
      901 tctgctcgtg aagcggcggg tggtatgccg gacccgacgc tgccggaggt gtccggaggt
      961 catggcaggg cagacgatat catcggactg tgacccgccc cggggggggg gatcatccgg
     1021 cgaaaaaaac gatcgctccg gacaccgccc cccgtctcac gcagagaaaa aattcccgtt
     1081 tcagggcagt taacatgtta actggctgtc cgggcatttt tgcggttttt atctttatta
     1141 ttcagtttgt tgtgcggaaa aaatgttaac
//
LOCUS       DBFIFS010000462         1167 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.472, whole genome shotgun sequence.
ACCESSION   DBFIFS010000462 DBFIFS010000000
VERSION     DBFIFS010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.472"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            186..1079
                     /gene="galF"
                     /locus_tag="N7A07_005043"
     CDS             186..1079
                     /gene="galF"
                     /locus_tag="N7A07_005043"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU2479488.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT          329 a          257 c          297 g          284 t
ORIGIN      
        1 actgattaaa ttatcagtat gtggatttcc gagtaatccg agtaacccag atgctgatgg
       61 tgtgtagtgt tatcgtaagg tttaatcaaa gttatgcgga actaagcttt ggtatatagc
      121 cataacgttt ggctaaccta tggcaataat ttacgcacta tcagccactt agaggtagga
      181 aaagaatgac gaatttaaaa gcagttatac cggtagcagg tcttgggatg catatgttgc
      241 ctgccactaa ggcgattccc aaagagatgc taccgatcgt cgacaagcca atgattcagt
      301 acattgttga cgagattgtg gctgcaggga tcaaagaaat cctcctggta actcacgcgt
      361 ccaagaacgc agtcgaaaac cacttcgaca cctcttatga attagaatct ctccttgaac
      421 agcgcgtgaa gcgtcaacta ctggcggaag tacaatctat ctgtccgccg ggcgtgacca
      481 ttatgaacgt gcgtcagggt gaacctttag gtttaggcca ctccatttta tgtgcgcgac
      541 ctgccattgg tgacaatcca tttgtcgtgg tgctgccaga cgttgtgatc gacgacgcca
      601 gcgccgaccc gctacgctac aaccttgctg ccatgattgc gcgcttcaat gaaacgggac
      661 gcagccaggt gctggcaaaa cgtatgccgg gcgacctctc tgaatactcc gtcatccaga
      721 ccaaagaacc gctggatcgt gaaggcaaag tcagccgcat tgttgaattt atcgaaaagc
      781 cggatcagcc gcagacgctg gactcagaca tcatggccgt tggtcgatat gtgctttctg
      841 ccgatatttg gccggaactg gaacgtactc agcctggtgc atggggacgt attcagctga
      901 ctgatgccat tgctgagctg gcaaaaaaac aatccgttga tgcaatgctg atgactggcg
      961 acagttacga ctgcggtaaa aaaatgggct atatgcaggc gtttgtgaag tatggactac
     1021 gcaacttgaa agaaggggcg aagttccgca aaggtatcga gaagctgtta agcgaataat
     1081 gaaaatctga ccgaatgtaa cggttgataa ggaaataata tcggcagtga aaaactatag
     1141 atcaataaat atcactgctg ttgagct
//
LOCUS       DBFIFS010000463         1148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.473, whole genome shotgun sequence.
ACCESSION   DBFIFS010000463 DBFIFS010000000
VERSION     DBFIFS010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.473"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1148
                     /locus_tag="N7A07_005044"
     CDS             join(<1..283,283..>1148)
                     /locus_tag="N7A07_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HGU2479489.1"
                     /translation="NTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETL
                     RVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGVRPP
                     LEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREI
                     QRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKA
                     VAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMET
                     TFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAM
                     AESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPP"
     misc_feature    273..389
                     /locus_tag="N7A07_005044"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          269 a          275 c          354 g          250 t
ORIGIN      
        1 aatactcgtt tttcccccga agtccgtcag cgggcgattc gtatggttct ggaaagtcag
       61 gatgaatatg actcacagtg ggcggcaatt tgttccattg ccccaaagat tggctgtacg
      121 ccggagactc tgcgtgtctg ggttcgccag catgagcggg ataccggggg cggtgatggt
      181 gggctcacca gcgctgaacg tcagcgtctg aaagagctgg aacgtgaaaa tcgtgaactg
      241 cgccgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc ggagttcgac
      301 cgcctctgga aaaaatgatg ccactgctgg ataagctgcg tgagcagtac ggggtcggac
      361 cggtatgcag cgaactgcat attgccccgt caacgtatta ccattgtcag caacagcgac
      421 atcatccgga taaacgcagt gcccgtgcgc agcacgacga ctggctgaag agagagatac
      481 agcgcgtata cgatgaaaat catcaggtgt acggtgtgcg taaagtctgg cgtcagttgt
      541 tacgggaagg aatcagggtg gccagatgta cagtggcacg tctcatggcg gttatgggac
      601 ttgccggtgt tctccggggt aaaaaggtcc gtacgaccat cagccggaaa gccgttgccg
      661 caggcgaccg cgtaaaccgt cagttcgtgg cagaacgacc tgaccagctg tgggtggctg
      721 attttactta cgtcagcaca tggcagggct tcgtctatgt ggcgtttatc attgatgtgt
      781 ttgccggata catcgtgggg tggcgggtct catcgtctat ggaaacgaca ttcgtgctgg
      841 atgcgctgga gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata
      901 aaggctctca gtatgtgtca ctggcctata cggagcgact aaaagaagcc ggattactgg
      961 catcaacagg gagtacaggc gactcgtatg acaacgcgat ggctgagagc atcaatggtc
     1021 tttacaaagc ggaggtaata caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg
     1081 ccacactaac gtgggtggac tggtataaca atcgacgatt gctgggaagg ctgggccata
     1141 ctcctccg
//
LOCUS       DBFIFS010000464         1145 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.474, whole genome shotgun sequence.
ACCESSION   DBFIFS010000464 DBFIFS010000000
VERSION     DBFIFS010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.474"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(258..905)
                     /gene="ychE"
                     /locus_tag="N7A07_005045"
     CDS             complement(258..905)
                     /gene="ychE"
                     /locus_tag="N7A07_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU2479490.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(936..989)
                     /gene="ychE"
                     /locus_tag="N7A07_005046"
     CDS             complement(936..989)
                     /gene="ychE"
                     /locus_tag="N7A07_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU2479491.1"
                     /translation="MLSKGVLSARLFNLIYG"
BASE COUNT          370 a          261 c          230 g          284 t
ORIGIN      
        1 ttatttagca ctttcctcta ccaaaggcag tggaattact aatatcattt caattattct
       61 tcagcaagcc caggattatg atgtggcgaa aataacatga tgatgaggag aagcagttaa
      121 ggaaatcctt ataactaact gtaatttgag aagaatattt ttacctattc cttttgcttt
      181 tgataactgc gcgggaacaa agtaaaaata atgacgaagt aaacggagca tggcagctcc
      241 gtttcatttg aaaggaatta attaagcagg ccggggaaaa tccccttaat accagtaacg
      301 ataaattcaa tccccaatgc catcagcaat agccccataa tacgcgtaat cacgttgatg
      361 ccggtctggc gtaaaacccg taccagccac ggtgccatgc ggaacaatcc ccaacaacat
      421 aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat agctaatgct gtgataacgc
      481 gtaccccaga cgatggtaga actgatcgcc cctggccccg ccatcaacgg caacgccaat
      541 ggcaccacac caatactttc acgtaccgcg gtttctgatt tttcttgctt gttctgttta
      601 tcctcgccaa gcttgccgct gatcatcgac atcgctatcg tcaccaccag gatgcccccg
      661 gcgatacgga acgaatcaat tgatatacca aaaagttgca gaatcgtgtc gccgagaaaa
      721 agcgagatcc acaagataat ggccacagac aggttggctg taaggttagt tttgtttcgc
      781 gctgccgctg tctgataact ggtcatgctg ataaagacgg gaataatccc taccgggttg
      841 accagcgcaa ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa aagggtctga
      901 atcacggtta gctccgaagc aaaagccgga taatgttagc cataaataag gttgaaaaga
      961 cgcgctgaca atacgccttt tgacagcatt tttcacctcc taactactta aaattgctat
     1021 cattcgttat tgttatctag ttgtgcaaaa catgctaatg tagccaccaa atcatactac
     1081 aatttattaa ctgttatcta taatggcgaa aagcgatgct gaaaggtgtc agctttgcaa
     1141 aaatt
//
LOCUS       DBFIFS010000465         1141 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.475, whole genome shotgun sequence.
ACCESSION   DBFIFS010000465 DBFIFS010000000
VERSION     DBFIFS010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.475"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(105..>1141)
                     /locus_tag="N7A07_005047"
     CDS             complement(105..>1141)
                     /locus_tag="N7A07_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU2479492.1"
                     /translation="EVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYARLGFKGETQ
                     INDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAGLKISEFGSLDYGRNYGVGYDIG
                     SWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
BASE COUNT          321 a          255 c          247 g          318 t
ORIGIN      
        1 tgaatgacca gaaatatcaa aaattgctca tttttactta aggagtcata cagcagcact
       61 taaatcaaaa accagcccag agaagggctg gtcaggagag gatgttagaa ctgataggta
      121 atgcccgtag cgacgatgtt atcggtgcga atatctacgg cacgggtaaa gtcgctttca
      181 tcaatcaggt tgattttata atcaacatag gtcgacatat ttttgttgaa gaaatacgtc
      241 gcaccaacgt cgatatactc aatcatgtcc tgatcgccgt agcggcccag atctatgcct
      301 ttcgatttca ggtaagcgag tgaggggcgc aagccgaagt cgaattgata ttgcgccacc
      361 acttcaaaac tttgtgcttt gttagaaata taaccatcgc cataggtggt catgtttaat
      421 gtttcagcgt aattagctgc aaagtagatg ttgttggcat catattttat acctgtagcc
      481 cacagttctg cattcttacc ggaggcattg agaggattag cagcggctct ttcctgcgca
      541 tttgtccgat cggatttggt atacaccgct ccgataccaa agccatcata aacataagtt
      601 gaggagatac cgaaaccgtc accgttggca cgcgtgtagt cagcaccata aagatgactg
      661 ttgtcagtac gttcattttt gccttgatat tgcgcggcaa agttcagccc ttcaattaag
      721 ccaaagaaat cgtagttgcg ataggttgct acaccggtgg tacggtatgt catgaagaca
      781 tctttctgac tccaggtatc gccaccaaat tctggcaaca tatcagtcca tgaaccaatg
      841 tcatagccga caccgtagtt acggccatag tccagagagc caaattcact aatttttaaa
      901 ccagcatagg ccagacgggt cttgttgtcg cgcgagccgt tggcttcatc gttatagcct
      961 ttaaaatcat attcccagtg accaaaacca atcatttgat cattgatttg cgtttctcct
     1021 ttaaagccga gacgggcata agttttatca ccatcgtcac gcttatcatc agtaaaataa
     1081 cgtagagcgg taaccttgcc ataaaggtcg agtttattac catccttgtt atagacttcg
     1141 g
//
LOCUS       DBFIFS010000466         1135 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.476, whole genome shotgun sequence.
ACCESSION   DBFIFS010000466 DBFIFS010000000
VERSION     DBFIFS010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.476"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..152)
                     /locus_tag="N7A07_005048"
     CDS             complement(<1..152)
                     /locus_tag="N7A07_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU2479493.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAG"
     gene            complement(377..931)
                     /locus_tag="N7A07_005049"
     CDS             complement(377..931)
                     /locus_tag="N7A07_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU2479494.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(994..>1135)
                     /locus_tag="N7A07_005050"
     CDS             complement(994..>1135)
                     /locus_tag="N7A07_005050"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2479495.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          276 a          318 c          213 g          328 t
ORIGIN      
        1 cccgccataa ccagctgggt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc
       61 tgcgcaatct ccccgactgt cgccggttcg gtaacgctta attcattaaa caccactctg
      121 gcggtttctg tcatatcctg ctgttttagc atgtcttttt cccttttccg gttaacgtga
      181 cataccaata actcttgtcg aaaaagccag caagttgaaa gaccggtatt agcaaccacc
      241 agcgcgttta acgccccgtg ccgtttttca gtcataaaaa aacccgcaaa aagcgggctc
      301 tttcaaatgt ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc ttaccggaat
      361 tgaagcagtt tttacgtcaa aaagcaataa cttttttctc tataccaaaa gccataacca
      421 ttggtttgta caaaataaat tctgccacct ttagccaatg ctcaatgcgt ctttcacagg
      481 ttcttaaact ccattccgga tgtgcatcat tcagcaattc agccattttg cgcttagtca
      541 tcccccgccc cacataacgc tgactcagaa cattgagcag cccgggataa cctgccagta
      601 cttcaccaat aaccctatcg attattaacg cctctgaatc ggtacaatgt gccagccagc
      661 ttttttgatt gccgttgatc atatcccgca aaaaagcctc aagttcaggt ttgtccagac
      721 ccgctttttt catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg
      781 tcagtaattg gttgaacata tttcccgtct taccgtcgcc aatatacgac caacgccccc
      841 acatacgcag tttcccctgg atccagacac tttccagcgt tttcaggcgt aaatgctcac
      901 cgcttttgcc tgtaatttcc gggtatatca tatttatgct cactcacttt caattttgta
      961 aatcttcacg cccagccgcc caccagaaac gagctagccg cgcacaatat tgatttcatc
     1021 aaactgctcg tcgtctatga gaagtccggc atgcgtcagc gcatccagtg gtgctttcag
     1081 gatattgtcc aggtcacgac ggcgcttatc cggtggctct gcaataatct ttatc
//
LOCUS       DBFIFS010000467         1130 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.477, whole genome shotgun sequence.
ACCESSION   DBFIFS010000467 DBFIFS010000000
VERSION     DBFIFS010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.477"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
BASE COUNT          344 a          194 c          224 g          368 t
ORIGIN      
        1 atttgaatgt tcgtaagcta tatcacttac tcaattcatt ttacccagag tcataaaata
       61 gagtgtctgt tgatctcatt ttcatattcc tttcaggctg aagtgcgttt tcatcacccg
      121 atgagaaaga actgaagtga tgtttgccat agattgcaag atgccctgtc tcattgtcta
      181 tcgtggtgca cctttatacc tgttatacca gatcgaaaat cacgcaatcc atacaacaaa
      241 accagatttg caattcgtgt cacaaaatat gtcgatcttt ttctaagaag aagatgccat
      301 gtgaagccag acgaacactt gcggtggtct tcaaaaacta aagatcttcg tttaactatt
      361 tgttttataa ataatttatt tagagtctaa acaagggaag ctttgcaagc taactcagtg
      421 agcttggtgt aaatcagtgt ttacccgcca tcaggctgag cataattctc atcatgaaat
      481 atgtttcctg gtttgtggct tgtaactggt cacttctgaa gtcgatctgg agaggcttat
      541 tgatgttggt gttttcagga tgatgtttca cttagtttgt ttgccgtatc gcccggcgaa
      601 tggctgtgat tgaggaaggt ttaagtcgta gtgaccaaag ctatatttac caacgaatgt
      661 agatgaaaaa atcatctcct gcgttccccc atatctccag gataaaaagg aatgtaacaa
      721 tctcatggcg taagctgacg aatcagccag gaataatcgc tagggaccta agaattagct
      781 tgataatagc cactaagaaa ttactgtgtt ccatgaaata gccattttgt ggcaaatgga
      841 gttgactaat aatgtcatat gtgagacggc tagttgaacg aatattaaat tttgctgaat
      901 tttttatgtt gattttactt gttacagaac atatcacatg atatatagat aagattagtt
      961 gcattaatga tgagggttat tattagattc gtatccgatt gataaatata taaaggtaca
     1021 tagcatgcaa gagcatagcg tttgtatggc aacgttatta taattaactg ttgctactcc
     1081 atttaagttc actcagaaga actggtccac ttacgttagt tattaagcaa
//
LOCUS       DBFIFS010000468         1123 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.478, whole genome shotgun sequence.
ACCESSION   DBFIFS010000468 DBFIFS010000000
VERSION     DBFIFS010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.478"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            117..224
                     /gene="yniD"
                     /locus_tag="N7A07_005051"
     CDS             117..224
                     /gene="yniD"
                     /locus_tag="N7A07_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU2479496.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(277..1035)
                     /locus_tag="N7A07_005052"
     CDS             complement(277..1035)
                     /locus_tag="N7A07_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU2479497.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          309 a          277 c          269 g          268 t
ORIGIN      
        1 aaacagtgat caggtgtaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg
       61 cctgtccctt ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc
      121 cgactaaacg ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg
      181 gcgctatgtt gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag
      241 acagccagac cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa
      301 ggttgtccga cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac
      361 gttgtaagcc accttcagtc caaaatgctc attaatagca acatttaatg cgctttcgga
      421 gttcagcgtt gtgtcttccg cgccaaagac ggaaacaccc tgcgtaaatt tagcgttgtc
      481 agtaagctgc catgcataag caccggaggc atagcccagc ggctgagttt cactggcatt
      541 gtcggtatat ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg
      601 gccattcaaa aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt
      661 ataacggtct gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa
      721 acggctacgc ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt
      781 actggcgttc ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc
      841 agccgtaagt gaggagcttt tcgtattgcc tgattgtgca agataccctg cgttcagatt
      901 accttcgaaa ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc
      961 ggcagctgca ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt
     1021 cttcaaaagc ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg
     1081 gaaactttct taaggatcaa agattagcgt ccctggaaag gta
//
LOCUS       DBFIFS010000469         1112 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.479, whole genome shotgun sequence.
ACCESSION   DBFIFS010000469 DBFIFS010000000
VERSION     DBFIFS010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.479"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1112
                     /locus_tag="N7A07_005053"
     CDS             <1..>1112
                     /locus_tag="N7A07_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479498.1"
                     /translation="GRETQREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLK
                     AQSAGGHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWD
                     SRDQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDEL
                     YSVRHLVFNGFELISQQCSRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTV
                     WRPGQTSLWGLALSLPADTDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYE
                     PETGMYLVSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGH
                     TGFLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYL"
BASE COUNT          267 a          277 c          376 g          192 t
ORIGIN      
        1 cggacgggaa acacagcggg agtaccggct tgaccggaac ggccaggtca cggcggtgac
       61 agcctcaggc accgggctgg gctatggcga aggcgatgag tcctatggct atgacagctg
      121 tggctacctg aaggcgcagt ctgcgggcgg gcaccggata agtgaagaga ctgaccagta
      181 tgccggaggc caccggctga aacaggccgg aaacacgcag tatgactatg acgccgcagg
      241 ccggatggtc agccgcacca gacaccgtga cggctatcgc ccggaaacag agcggttccg
      301 gtgggacagc cgggaccagc tgaccgggta ttgcagcgca cagggtgagc agtgggaata
      361 ccgccacgac gccagcggca gacgaacaga gaaacgctgc gaccggaaga aaatccgctt
      421 cacgtacctg tgggacggcg acagtattgc ggaaatccgg gaataccgcg atgatgaact
      481 gtacagcgta cggcacctgg tgtttaacgg ctttgagctg ataagccagc agtgcagccg
      541 ggtacggcag ccgcacccgt ccgtggcccc gcagtgggtg acacgaacga atcatgcggt
      601 gaacgacctg acgggccgtc cgctgatgct ctttaacagt gaaggtaaaa ccgtctggcg
      661 gccgggacag accagcctgt gggggctggc actcagcctg cccgcagaca cagactaccc
      721 ggacccgcgc ggggagcggg acgcggaagc cgaccccggc ctgctgtatg caggacagtg
      781 gcaggatgca gaatcggggt tgtgctataa ccggttccga tattacgagc cggaaaccgg
      841 gatgtacctg gtgagtgatc cactggggtt gcagggaggg gagcagactt accggtatgt
      901 gccgaatcct tgtgggtggg ttgatccgct gggattggct gcaagttcta aaatcagcag
      961 tttgatggac tatattggcg atggtcgtcg tgttagtggg catacgggtt tcctggatgg
     1021 ggttcgttta tcacgtagtc aaataaacaa tattgctaaa gaaatggaga agctaggaat
     1081 taaagtaata aggaaagcag ataaatattt gc
//
LOCUS       DBFIFS010000470         1105 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.480, whole genome shotgun sequence.
ACCESSION   DBFIFS010000470 DBFIFS010000000
VERSION     DBFIFS010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.480"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1105
                     /locus_tag="N7A07_005054"
     CDS             <1..>1105
                     /locus_tag="N7A07_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU2479499.1"
                     /translation="LMQVITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPD
                     SYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQY
                     GCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGV
                     GKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLAQFRAD
                     LSVFNAQCHGGSAADVPWICGDTTYYWKNTYGTQYNTIYGAYKNRESEGVYFVPFMTD
                     GNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATA
                     ILNAAGRTSAFISGKAPEIKPSPGGNTPSGPSA"
BASE COUNT          255 a          292 c          331 g          227 t
ORIGIN      
        1 ccctgatgca ggtgattaca gcagaaggtg atgtggtggt cagtggtgca actgagccgg
       61 attggtacta cgtcatcgta ctggccgggc agtccaatgc catggcttac ggtgaagggc
      121 ttccgctgcc ggattcatac gatgctccgg atccgcgcat taaacagctg gcgcgccgca
      181 gtacagttac gccgggtggg gctgcctgca gatataacga tattattccg gccgaccact
      241 gcctgcatga tgtgcaggat atgagtacgc tgaatcatcc gaaggcagac ctgagcaaag
      301 ggcagtacgg ctgtgtcggc cagggcttac atattgccaa aaaactgctt ccgtatatcc
      361 cgaataacgc ggggatcctg ctggtaccat gctgtcgtgg tggttcggca ttcacccagg
      421 gcgcggaggg gacattcagt gcggacgcgg gggccagcca ggattcggcg cgctggggtg
      481 tgggtaaacc gttatatcag gacctgattg cgcgcactaa agctgcatta cagaagaacc
      541 cgaaaaatgt gttgctggcg gtgtgctgga tgcagggaga gtttgacatg agcgccgcca
      601 cccacgcaca gcaacctgcg ctgtttacag ccatgctggc acagtttcgt gctgacctct
      661 ccgtgtttaa cgcgcagtgc catggtggca gtgctgcaga tgtgccgtgg atttgtggtg
      721 acacgacgta ttactggaaa aatacctacg gcacccagta caacaccatt tacggggcgt
      781 acaaaaacag ggagagtgag ggcgtttatt ttgtgccctt catgacagac ggtaacggcg
      841 tcaataccgc cactaacgcg ccggcagaag atccggatat tccggcatca ggatattacg
      901 gtgcggcatc gagaacgaat ggaaaccagg tatcatcaaa ccgcccgaca catttcagtt
      961 catgggcgcg caggagcatt attccggatc gtatggcaac cgctattctg aacgcagccg
     1021 ggcgcacctc agccttcatc agtggtaagg caccggaaat caaaccctcg cccggcggca
     1081 acacgccatc gggtccgtct gcaga
//
LOCUS       DBFIFS010000471         1087 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.481, whole genome shotgun sequence.
ACCESSION   DBFIFS010000471 DBFIFS010000000
VERSION     DBFIFS010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.481"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            10..255
                     /locus_tag="N7A07_005055"
     CDS             10..255
                     /locus_tag="N7A07_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2479500.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            333..548
                     /locus_tag="N7A07_005056"
     CDS             333..548
                     /locus_tag="N7A07_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2479501.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            553..1086
                     /locus_tag="N7A07_005057"
     CDS             553..1086
                     /locus_tag="N7A07_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2479502.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          258 a          250 c          344 g          235 t
ORIGIN      
        1 gaaaatccca tgtcagaaat tacatccctg gtcactgctg aagcagtgaa ggaagtcctg
       61 cgctctgaag aagtccggag cgcactgaaa cagaaacttc gccataacct ggaagcgcgt
      121 cttgatgcag aagtggatgc cattctggat gaactgctgg gcgcaccggc agctccggag
      181 ccggaaggca tcgcgggtga ggggagtgct tcagatagcg gtgaccccac accggacagc
      241 gacatgatga tgtaagcatg cgtcagggac catcggtgtg tgccggtggt cttttttatt
      301 gttgtgagct tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacag
      361 gtgtgtcata caccacgtca gcggtgggaa cgggctactg gttcctgcag ttgctggaca
      421 gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg tttgggctgc
      481 tgacatatct gacgaacctg tatttcaaga ttaaagaaga ccggcgtaag gcggcacggg
      541 gagagtaagc tgatgagcag gaaactccgc tatggtttat cggctgccgt tctggcgctg
      601 attgccgcag gtgcttctgc gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt
      661 aaccacacca cagcataccg tgatggtgcg ggtatctgga ccatctgccg tggagccacc
      721 cgggtggatg gtaagcctgt tattcctggc atgaagctgt cgaaggaaaa atgcgaccgg
      781 gttaacgcca ttgagcgtga taaggcgctg gcatgggtgg agaaaaacat taaagtgccg
      841 ctgaccgaac cccagaaagc ggggatcgcg tcattctgtc cgtacaacat tggtcccggt
      901 aagtgtttcc cgtcgacgtt ttacagacga attaatgcag gtgatcgaaa aggtgcctgc
      961 gaagctattc gctggtggat taaggacggt ggcagagact gccgtattcg ttcaaacaac
     1021 tgttacggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac
     1081 agataag
//
LOCUS       DBFIFS010000472         1084 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.482, whole genome shotgun sequence.
ACCESSION   DBFIFS010000472 DBFIFS010000000
VERSION     DBFIFS010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.482"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1084
                     /locus_tag="N7A07_005058"
     CDS             <1..>1084
                     /locus_tag="N7A07_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2479503.1"
                     /translation="NLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLN
                     KINACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFP
                     YNVRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAI
                     TQFHELLSKLHHKPLLDSKGENISSDEPISLTTDLLLWRSSWPTLATAIRQFSQGIVS
                     NEIETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGR
                     SDSPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLL
                     TINGDGHTLALTGVNLCVDKAVVHHL"
BASE COUNT          332 a          242 c          231 g          279 t
ORIGIN      
        1 caaatcttcg tgagtcatgg gatattattg gatttgatcc tcgaggcgtc gggcagtcca
       61 ctccgacaat aaactgccgg caatcagata cagagactca ggaaaacata accgaaaagc
      121 aacaagtatt aaataaaatt aatgcctgta tccataatac cggagccgaa gtcattcgcc
      181 atatcggctc taacgaggct gtatacgata ttgatcgtat taggcaagcc ttgggggata
      241 aacaactgac agccgtggcg tattcgtatg gaactcaaat tgcagcctta tatgcagaac
      301 gttttcccta caacgtaaga tctatcgttc ttgatggagt cgtcgatatc gatgacctgg
      361 aggacaactt cacatggcaa ctcaaacagg cacagagtta tcaggaaacg tttgatcgct
      421 ttgcatcctg gtgtgcgcgt acaaaaagtt gcccgctttc ttcagacaga gataaggcaa
      481 taactcagtt ccatgagcta ttatcaaaat tacatcacaa acctttatta gacagtaagg
      541 gagaaaatat atcttcagat gaacccatat cattaacaac agaccttctg ctatggcgtt
      601 catcatggcc aacccttgca actgccatac gccagttctc tcaggggatt gtcagtaatg
      661 aaattgaaac tgcgctcagt gctccgatag cctcagaaga gtcaagtgat gcttcggggg
      721 taatcctttg tgtagatcag ggggatgagc aattaacacc agaagagcga aaattccgaa
      781 aagaggctct tgcgaatgcc ttcccggcta ttaactttga caatggacgt tccgattcac
      841 ctgatttttg tgaattatgg ccaatacata gcgacctgaa caaaactcgc ctgaaaaata
      901 ctgttctgcc ctctggttta ctgtttgtag cacacaaata cgacccaaca acgccctgga
      961 ttaatgcccg taagatggca gagaaatttt ccagcccgtt actaacaata aatggtgatg
     1021 ggcatacatt agctctcacc ggagttaatt tatgtgtaga taaagcagtt gtacatcacc
     1081 tgat
//
LOCUS       DBFIFS010000473         1066 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.483, whole genome shotgun sequence.
ACCESSION   DBFIFS010000473 DBFIFS010000000
VERSION     DBFIFS010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.483"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1066
                     /locus_tag="N7A07_005059"
     CDS             <1..>1066
                     /locus_tag="N7A07_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU2479504.1"
                     /translation="DSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDA
                     WGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRY
                     HHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEG
                     HRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVE
                     WLTYGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSL
                     QYDRDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNR
                     LPDPELHPDSTLSMWPDNRIA"
BASE COUNT          255 a          345 c          314 g          152 t
ORIGIN      
        1 cgacagccgt ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga
       61 atacaacgcc gccggtgacc tgaccaccgt cattgccccg gacggcagca gaaacgggac
      121 acagtacgat gcgtggggaa aagccatctg taccacgcag ggcggtctga cgcgcagtat
      181 ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac
      241 cttccgttac gatgtactcg accggctgat acaggaaacc ggctttgacg gccgcacaca
      301 gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac
      361 ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc
      421 agagcgctgg cagtatgacg aacgcggctg gctgacagac atcagccata tcagcgaagg
      481 gcaccgggtg acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca
      541 gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca gacacgctta
      601 caacgcgcag gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct
      661 gacctatggc agcggctggc tggcaggcat gaagctcggc gacacaccgc tggtggattt
      721 cacccgcgac cgcctgcacc ggaaaacgct gcgcagattc ggccgttatg aactcaccac
      781 cgcttatacc cctgccgggc agttacagag ccagcacctg aacagcctgc agtatgaccg
      841 cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg
      901 gagttacagc tacagcgact ccggcaggct gaccggcgtt cacaccaccg cagcgaatct
      961 ggatatccgc atcccgtatg ccacggaccc ggcaggtaac cgcctgcccg acccggagct
     1021 gcacccggac agcaccctca gcatgtggcc ggataaccgt atcgcc
//
LOCUS       DBFIFS010000474         1066 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.484, whole genome shotgun sequence.
ACCESSION   DBFIFS010000474 DBFIFS010000000
VERSION     DBFIFS010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.484"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>1066
                     /gene="espX6"
                     /locus_tag="N7A07_005060"
     CDS             <1..>1066
                     /gene="espX6"
                     /locus_tag="N7A07_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HGU2479505.1"
                     /translation="FTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEI
                     IECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSER
                     LTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKM
                     ALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNF
                     KNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPQIPGT
                     PKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISNANFSNAKFYNT
                     NCTGANCSNILFDYAWFDNTI"
BASE COUNT          366 a          207 c          191 g          302 t
ORIGIN      
        1 gtttacaaac ttacaatggg aaaatttgct gcaatactac aaagaaaacc accagcagcc
       61 aaatatagca ggatcacgaa atctcacgga taatatagat aaaattaaaa atacaatatc
      121 cacctctgaa attattgaat gcgcctctcc tgaaataaga agtagcgtcc tgaacgatct
      181 ttatagcatt gctaatttcc tcccggacaa taatctgacc ccaaatgaga gctggaaaag
      241 attttgtgag acatgcgagc gcttttacgt tgctcagaag agtatcactg gagataagag
      301 tgaacgtctt acgcgaaaac tctctatctc tgatgcagga attacaatga ccttcaagat
      361 aggtgatgtt gtcatcaata ctattagcac tgctattcct gaagatgcaa cgggtcaacg
      421 gtgtatcgaa gggttgaatt tagcagagat ggatttaacc gacatagact tgtcgaaaat
      481 ggcgctaagg aatgtcaatt ttaatggcag cattcttaga aatgccaagt tctccggtac
      541 gatctgtgaa ggcgtggatt ttaccgattg tgatctgcgt aatgcagaat tcgaaaatgc
      601 ctcattagaa aataatgatt ttcgtaaagt tcgccacttg acttatgtaa atttcaaaaa
      661 cgcaaatcta cgaaacagta acttcaacgg aaaagttctc actggcgtaa cctttactgg
      721 aagtgacctt agtaacgcgt atcttgaaca catagatttc acaaccgtga ttctatatga
      781 aacatctaaa atacctggaa tacctggaac acctcaaata ccgggaacac ctaaagtaat
      841 tcttactggc gcaatactaa attattccga tctatcggga aaagatcttt cagaatataa
      901 tcttactggt attctctgca tgtataccaa cttttcaaac gctaatttaa caaattgtaa
      961 aatctctaat gcaaactttt cgaatgcaaa attctacaat actaattgta ctggtgcaaa
     1021 ttgttcgaat atcctatttg actacgcatg gtttgacaat acaata
//
LOCUS       DBFIFS010000475         1065 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.485, whole genome shotgun sequence.
ACCESSION   DBFIFS010000475 DBFIFS010000000
VERSION     DBFIFS010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.485"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..224)
                     /locus_tag="N7A07_005061"
     CDS             complement(<1..224)
                     /locus_tag="N7A07_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2479506.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTL"
     gene            complement(239..955)
                     /locus_tag="N7A07_005062"
     CDS             complement(239..955)
                     /locus_tag="N7A07_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU2479507.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
BASE COUNT          241 a          328 c          251 g          245 t
ORIGIN      
        1 aacagcgtca catccgcttc caggttacgg cgggttttcg ccaccaccga cagggtatcg
       61 tcatcctctc catcaccatt gagcacttcc tgccacagat accaggcctc tgccgaaggc
      121 tcccgcagca ccacgctgac attaccccat tccggcacct tcaccgtttt atgacgaaac
      181 cctgacagtc tggccagcgc cagcgttttc agatcctttt tcatgatgac ccatcccctt
      241 atccggcggc tgcgctcact gtcacggtgc attcaacaga cgtcacactc tgtgctttct
      301 ctgccgaatc ggtcaccaca caggtatatt tccccgcatc agcggactgc gcacctggct
      361 tactgaaggt gtctgtcgtc tgcccgtcaa ccggctgacc atccttcttc caggcgtatt
      421 tatacggcgg cgttcccccg ttggcactga ctgacattgt cagcagcgca ccggtattca
      481 cggtaagtgt cttatccaga tttttcacaa acgccagcgg taccacaaag gacaccggtt
      541 tgcctttcag acgcagtgag aacgttgcag ccaccacgcc gttggtaccg gatgaccagg
      601 tgtgctggcg cacttccgcc aggaatttaa agcccttacc ggacggaaac agcaccttaa
      661 acgcatacaa cgcgtcattg tcataggcat cacgcagggc gttctgggcc tgattcagat
      721 aaaaattgcc cgacatggaa atctcagacg acgcccccag accattgatg ttctcctgct
      781 ctgtggagca gagcgtggtc acatcaatat cctgtttctg cccggcggtg aactgcactt
      841 ccttgatggt gcagtccagg cgcagatatt ccgccttatc catagtttca gcagtcgccg
      901 gggcagatga aatcatcacc tgcgtcagct gtgagcgttc atacaaagca gacattctgc
      961 ctcctgataa taaaaaaccc gcacgcggcg gggtatgggt tttgtagaaa aaagaaaaag
     1021 tcacaccgtg acctgaaact ccagggttgc acggtaacag cggtt
//
LOCUS       DBFIFS010000476         1065 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.486, whole genome shotgun sequence.
ACCESSION   DBFIFS010000476 DBFIFS010000000
VERSION     DBFIFS010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.486"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            796..1065
                     /locus_tag="N7A07_005063"
                     /pseudo
     CDS             796..1065
                     /locus_tag="N7A07_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          330 a          204 c          210 g          321 t
ORIGIN      
        1 gagtggttat tcgcatatta ctaatctccg atcatgttaa ccggcaagac aatacacaaa
       61 tacaaacaat aatgagaata atttatattt gccttttaca aaaaataaag attattgaca
      121 aaaccaaata taaataacga ctgcctcggc atgccagtta acagctatcc gtatggcgtg
      181 ttcggttttg cgaataaaaa atggttactg tagaagcgga tttccaccac atgtcaacca
      241 tgaatggtca gtgatgcgag acgactggga tgatttggtc gatgacttct tccggcgaat
      301 actgatagaa acattgaaat attgtaagag aaagcgttca aaattctgtg gcacgtgaaa
      361 aattctacag cgtcatcacc ttattcagtc gaccttcaaa ataaacgttt aacagagaca
      421 atcaccattt ggattgtccg ctgaggaggc gaacaatttc ctagacagct ttatcatgtc
      481 agcccacatt gttggcgctg gcaatactaa ggacaaacga tggggaagta gctttttagc
      541 gttattttcg ggagtaaaaa acgtggtatc tttccgtatg tcagttgtat cttgctggta
      601 ttcgtagtcg tgataaggca tacaacgata tttatcttga cgttagtgaa gaataagttg
      661 ttttgtgatc tatgttaatc tgggattaca atccgggctt gatacatcgc atcacgactt
      721 ttattctctt cagaatagaa tatgttactc cccgctttac aggctcagga tttgaattac
      781 aaattatact tttaagtgaa caataaccaa gggtatttct tatttcctga aaccaggctt
      841 ggcccatttc gggatgagtt tgaaaaaact cttgacgcat acgatagtga tgaaataaac
      901 gaaacaaatt acgtcaacaa actcaggcgt cttgcctgac atgctcctga catcattgat
      961 attcacgctc atctggcata tgcatttgcg ctctttatgc tgcaattctg gcgaatgttc
     1021 atttacagcg tcatcaggat gccgttatac tcattgataa aatac
//
LOCUS       DBFIFS010000477         1057 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.487, whole genome shotgun sequence.
ACCESSION   DBFIFS010000477 DBFIFS010000000
VERSION     DBFIFS010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.487"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            3..218
                     /gene="sctS"
                     /locus_tag="N7A07_005064"
     CDS             3..218
                     /gene="sctS"
                     /locus_tag="N7A07_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HGU2479508.1"
                     /translation="MLSLPTVIAASVIGIIISLVQAITQLQDQTLPFLLKIIAVFATL
                     ALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            218..994
                     /gene="escT"
                     /locus_tag="N7A07_005065"
     CDS             218..994
                     /gene="escT"
                     /locus_tag="N7A07_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HGU2479509.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            987..>1057
                     /locus_tag="N7A07_005066"
     CDS             987..>1057
                     /locus_tag="N7A07_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479510.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVT"
BASE COUNT          315 a          189 c          182 g          371 t
ORIGIN      
        1 ttatcctctc attgcctaca gtcatagcgg cctctgttat cggtattatt attagtctgg
       61 tccaggctat aacgcagtta caggatcaaa cattgccttt tttgctaaaa ataatagcag
      121 tgtttgctac gcttgccctg acttatcact ggatgggaac aacaatcatc aacttctcgt
      181 cgataatatt tgaaatgatt ccgaaggtga acggctaatg aatgagataa tgacggtcat
      241 agtatcatca ttttattgta tactcaggcc gctggggatg tttattatcc taccaatatt
      301 ttcaacaggt gttttactca gtaactttat tcgcaactca attatgattg cgtttacgct
      361 tccgatcata gtagagaact atacattttc ggaaaaattg ccatctggaa tctttcagtt
      421 gacaggaata gcgctaaaag aaataagtat tggttttttc attgggttat catttactat
      481 tcttttttgg gcaatagatg cggctggaca gattattgat actctaagag gctcaacaat
      541 atcttcaatt tttaacccgt ccataagtga ttcatcttct atcactggcg ttatcttgta
      601 ccaatttatc tctgtgatct ttgttattca tggtgggata caaagcatcc tagataagct
      661 atatttatcc tacgagatat taccattaca agccgatatt gcattcaatc gtgctttaat
      721 agattttttg ttttctctat gggattcatt tattaaactg atgttatcat tttcagttcc
      781 catgattatc ggtatattct tatgtgatat ggggtttggg tttcttaaca aaacagcacc
      841 tcagctaaac gtattcacat tatcactgcc agtaaaaagt ttgatagcaa tctttatatt
      901 gctattagtt attcatgttt ttcctgattt tattacggca aatattcatt ctgacataat
      961 tgatagatcg ttaccgagca tgattaatga gtgaaaaaac agaaaagccc acacccaaaa
     1021 aactaaggga tctaaaaaag aagggcgatg taacaaa
//
LOCUS       DBFIFS010000478         1041 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.488, whole genome shotgun sequence.
ACCESSION   DBFIFS010000478 DBFIFS010000000
VERSION     DBFIFS010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.488"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            124..306
                     /locus_tag="N7A07_005067"
     CDS             124..306
                     /locus_tag="N7A07_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479511.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            284..556
                     /locus_tag="N7A07_005068"
     CDS             284..556
                     /locus_tag="N7A07_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU2479512.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            615..866
                     /locus_tag="N7A07_005069"
     CDS             615..866
                     /locus_tag="N7A07_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479513.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
BASE COUNT          338 a          231 c          224 g          248 t
ORIGIN      
        1 ctaaaaaaca atactattaa atacaaagcg ttgtaaaaaa acgccccgtt ttagaacaaa
       61 ttgtattgac caagtgaagt acatatcgta ctatttaacc atcagcagga cgctggaagc
      121 caaatggaac agactggcag gctctttaaa caacgtcgac tctcgactac gtggctgaaa
      181 agccagatca cccaaccaca taagctgtgg gatgcaatgc cgaagcaacc gtctcaggag
      241 gagcttcgag attgcatcgc caaagtttat tcgggaggaa tctatgtcca gaaaaacaga
      301 atttaaaggc accgcagctt ctcgccgtag agctcgtcgc gcaaacctgc aaagtcagga
      361 ggcgattagc tccgacaaac tacacaggcc aaccccatca cgagtggtct tacaatgcaa
      421 acgcaaacca gcaatgagag cagaagtaat aacactgaca acgttgacca gaaaatatga
      481 aggctcaact tgtcttccaa atgtagctct ttacgcggca ggctaccgta aatccaaaca
      541 actgacagca agatgacttg tgttggtcgc cagaaaatga aattaggcag caaaccactt
      601 atttgaggtg atatatggaa tttcatgaaa gtgcgatttg tgattttcgc gctaacgcaa
      661 attcagtaaa accacagcca attgcagttc tttttaaaac aatgggtgcg tgggctgttt
      721 tatgcttcgc cgctgacgac actgacgcaa gaatggcaat aggacaagag atggagatgg
      781 acccgacaaa cgatgaattc ataatttatg gcgctccatc taattactta cttgatacct
      841 gcaacattta caacaaggct gcctgatggt ggcctttatt tttggcataa acaacagaat
      901 aaacacagca ctgtgtattc attccaacga gtgaatacac ggagcaatgt cgctcgtaac
      961 taaacaggag ccgacttgtt ctgattattg gaaatcttct ttgccctcca atgtgagggc
     1021 gattttttat ctgtgaggat a
//
LOCUS       DBFIFS010000479         1039 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.489, whole genome shotgun sequence.
ACCESSION   DBFIFS010000479 DBFIFS010000000
VERSION     DBFIFS010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.489"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            134..>1039
                     /gene="ompT"
                     /locus_tag="N7A07_005070"
     CDS             134..>1039
                     /gene="ompT"
                     /locus_tag="N7A07_005070"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU2479514.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGI"
BASE COUNT          355 a          208 c          219 g          257 t
ORIGIN      
        1 aagaataaat aaaacgaaac aataattcaa acttaaacaa accataaaca gttaaacaat
       61 atacaattga ctcattaagt tagatataaa aaatacatat tcaatcatta aaacgattga
      121 atggagacct tttatgcggg cgaaacttct gggaatagtc ctgacaaccc ctattgcgat
      181 cagctctttt gcttctaccg agactttatc gtttactcct gacaacataa atgcggacat
      241 tagtcttgga actctgagcg gaaaaacaaa agagcgtgtt tatctagccg aagaaggagg
      301 ccgaaaagtc agtcaactcg actggaaatt caataacgct gcaattatta aaggtgcaat
      361 taattgggat ttgatgcccc agatatctat cggggctgct ggctggacaa ctctcggcag
      421 ccgaggtggc aatatggtcg atcaggactg gatggattcc agtaaccccg gaacctggac
      481 ggatgaaagt agacaccctg atacacaact caattatgcc aacgaatttg atctgaatat
      541 caaaggctgg ctcctcaacg aacccaatta ccgcctggga ctcatggccg gatatcagga
      601 aagccgttat agctttacag ccagaggtgg ttcctatatc tacagttctg aggagggatt
      661 cagagatgat atcggctcct tcccgaatgg agaaagagca atcggctaca aacaacgttt
      721 taaaatgccc tacattggct tgactggaag ttatcgttat gaagattttg agctaggtgg
      781 cacatttaaa tacagcggct gggtggaagc atctgataac gatgagcact atgacccagg
      841 aaaaagaatc acttatcgca gtaaagtcaa agaccaaaat tactattctg tttcagtcaa
      901 tgcaggttat tacgtaacac ctaacgcaaa agtttatgtt gaaggcacat ggaatcgggt
      961 tacgaataaa aaaggtaata cttcacttta tgatcacaat gataacactt cagactacag
     1021 caaaaatggt gcaggcata
//
LOCUS       DBFIFS010000480         1030 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.490, whole genome shotgun sequence.
ACCESSION   DBFIFS010000480 DBFIFS010000000
VERSION     DBFIFS010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.490"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..819
                     /locus_tag="N7A07_005071"
     CDS             <1..819
                     /locus_tag="N7A07_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGU2479515.1"
                     /translation="DVSPALVSKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQ
                     DSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVD
                     GLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQ
                     QALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIRKVIYTTNAIESLNSVIRHA
                     IKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(869..>1030)
                     /locus_tag="N7A07_005072"
     CDS             complement(869..>1030)
                     /locus_tag="N7A07_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2479516.1"
                     /translation="RVSKRYDDVEVIIKSTSNDGLSVTRTADKDSAKTFVQETLKDTW
                     ESADEWFVR"
BASE COUNT          260 a          269 c          276 g          225 t
ORIGIN      
        1 gatgtctcac cggcgctggt atcgaaggtc accgatgccg ttatggagca ggtagtcgaa
       61 tggcaaaacc ggccactgga tgcggtctat cccattgttt atcttgactg tattgtcctg
      121 aaggttcggc aggacagtcg cgtaatcaat aaatccgtat tcctcgcact gggtatcaat
      181 atcgaaggcc agaaagagtt actgggtatg tggctggccg aaaacgaggg agcgaagttc
      241 tggctcaatg tgctgactga actaaaaaac cgcggcctga acgatattct catagcctgt
      301 gtcgacgggc tgaaaggctt cccggacgct atcaacacgg tgtatccgga agcccgcatc
      361 cagctgtgca tcgtgcatat ggtgcgcaac agcctgcggt tcgtttcctg gaaggactac
      421 aaagccgtca cccgcgacct gaaagccatc taccaggccc ccacagaaga agcaggccag
      481 caggcgctgg aagcgttcgc cagcgcctgg gacagtcgct acccgcagat aagccggagc
      541 tggcaggcaa actggaccaa cctggcgatg ttcttcgctt acccggcaga tatccgcaaa
      601 gtcatctaca caaccaacgc catcgagtcg ctgaacagcg taatccggca tgccatcaaa
      661 aaacgcaagg tgttcccgac ggatgactca gtgaaaaagg tcgtgtggct ggcaatccag
      721 gcggcttcac agaaatggac gatgccactg cgggactggc gtatggcaat gagccgcttt
      781 attatcgagt tcggtgaccg cctggacggt cacttctgag aaaaggcatt tacacagaat
      841 cgtgtacagg gtcgatttta ctcgttaatt agcgaacaaa ccactcgtca gcagactccc
      901 aggtatcttt cagcgtctcc tgaacaaaag tttttgcaga atctttatcg gcggtgcgag
      961 taactgaaag gccatcgttg ctggtggatt tgatgatcac ttctacatcg tcataacgtt
     1021 tactgacacg
//
LOCUS       DBFIFS010000481         1028 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.491, whole genome shotgun sequence.
ACCESSION   DBFIFS010000481 DBFIFS010000000
VERSION     DBFIFS010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.491"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..778
                     /gene="bet"
                     /locus_tag="N7A07_005073"
     CDS             <1..778
                     /gene="bet"
                     /locus_tag="N7A07_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2479517.1"
                     /translation="ALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFIAL
                     LIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESCT
                     CRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYNKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLL
                     PLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            775..>1028
                     /locus_tag="N7A07_005074"
     CDS             775..>1028
                     /locus_tag="N7A07_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU2479518.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQY"
BASE COUNT          274 a          271 c          273 g          210 t
ORIGIN      
        1 agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc
       61 acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca
      121 gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga cgaaagaaat
      181 ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg atggctggtc
      241 ccgcatcatc aatgaaaacc agcagtttga tggcatggac tttgagcagg acaatgaatc
      301 ctgtacatgc cggatttacc gcaaagaccg taatcatccg atctgcgtta ccgagtggat
      361 ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca ccggcccgtg
      421 gcagtcgcat cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctggc
      481 cttcggattt gctggtatct ataacaagga tgaagccgag cgcattgtcg aaaatactgc
      541 atacactgca gaacgccagc cggaacgcga catcactccg gttaacgatg aaaccatgca
      601 ggagattaac actctgctga tcgccctgga taaaacatgg gatgacgact tattgccgct
      661 ctgttcccag atatttcgcc gcgacattcg cgcatcgtca gaactgacac aggccgaagc
      721 agtgaaagtt cttggattcc tgaaacagaa agcctctgag cagaaggtgg cagcatgaca
      781 ccggacatta tcctgcagcg taccggaatc gacgtgagag ctgtcgaaca gggggatgat
      841 gcatggcaca aattacggct cggcgtcatc accgcttcag aagttcacaa cgtgatagca
      901 aagccccgct caggaaagaa gtggcctgac atgaaaatgt cctacttcca caccctgctt
      961 gccgaggttt gcaccggtgt ggctccggaa gttaacgcta aagcactggc ctggggaaaa
     1021 cagtacga
//
LOCUS       DBFIFS010000482         1025 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.492, whole genome shotgun sequence.
ACCESSION   DBFIFS010000482 DBFIFS010000000
VERSION     DBFIFS010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.492"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..96)
                     /locus_tag="N7A07_005075"
     CDS             complement(<1..96)
                     /locus_tag="N7A07_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479519.1"
                     /translation="MVCLYNFGVRAAMDNSKHSDEAEKLLAELSAR"
     gene            complement(129..>1025)
                     /locus_tag="N7A07_005076"
     CDS             complement(129..>1025)
                     /locus_tag="N7A07_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479520.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEELGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          240 a          291 c          200 g          294 t
ORIGIN      
        1 tctagcagaa agctccgcca ataatttttc cgcctcatca gaatgttttg aattatccat
       61 agcagccctc actccaaagt tataaagaca taccatgata atcaacaaaa aagtaaatac
      121 tttcattact atttgcttcc tgtaggtgta aacttatgaa agtcccctgg ttgtttctcg
      181 tctggcacca attcaaacac cccgttgtcc ttaccgccta ttgttatcct agctgtttgc
      241 cccataatag catagtttcc atccggccaa atccatatta tttcatcagg agcaatcact
      301 gtagcatcaa gttcgttagc tagtttctgt gcaaatgaat ttttgccttt tcctgtttcg
      361 caggaaaaca agactacagg cataccttca tgatatttag gattcgctct tatgtcgcta
      421 gctaattgtt ttattgtcag atcacttcct tttttgattc tgtcatcact catagatgta
      481 ggcgaaccat gacctccaac tgtaaaaccc gttggaaatt cccttacatt aagagaacct
      541 ttatatatag gatctgacgg agaataataa ttcaaatcaa gtgctaaccc caacggatct
      601 atatgttcaa taggattgag tgggtactga tacaggttcc atcccccctc cagccctatc
      661 gggtcctggg tgatatatct cccctgcaac ggatcatagt accggttgcg gttgtagtac
      721 agccccaact cctcatcata ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc
      781 gcgctggttt cgcccagcag gtttccccac tcgtcatact ccccctgcca cgccgtttcc
      841 ccttccgggc tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgaagt
      901 ttcctctccg ggatgtattc cgcctccagc tgcgcggcca tctgctccgc cgtcagcccg
      961 cactgcgcaa gccactgctg gctttcttca ctcacgctgc cctgccgcag ctcccgctcc
     1021 agcct
//
LOCUS       DBFIFS010000483          988 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.493, whole genome shotgun sequence.
ACCESSION   DBFIFS010000483 DBFIFS010000000
VERSION     DBFIFS010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.493"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(135..746)
                     /gene="map"
                     /locus_tag="N7A07_005077"
     CDS             complement(135..746)
                     /gene="map"
                     /locus_tag="N7A07_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HGU2479521.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          278 a          210 c          163 g          337 t
ORIGIN      
        1 gaaaaccaat taagcaaact ataaatctta attaataata aagtttatta agtttcttta
       61 tcgcgttttt tcttacatca acaaaggcca tgctatacac caggaagctt aatttatacc
      121 tgacaaggtt atccctacaa tcgggtatcc tgtacatgct ctaaaatcgc attctgagta
      181 atcgctttta tctcactgac tttgaagccg ctaaagttgc gttcaaatat ttcttgtgag
      241 acttgttcta tcatcttcgt ctgggtgtta aaacgtgtta ttggatccac tcttttgccg
      301 cactgctcat caactttctg attaaagtat ttattggact gcaagaagtg accaattgat
      361 gattgtgccc cgttcatatc aagaggttgc tgtaacgcat tttcaacagc tctaaaaatg
      421 tgacctttag tctccttact aatgaccgaa ttattactcc tgcaatagtc atccagagtt
      481 ctatttactg ttctggatat ataagtcgtc tgctcctgct ggaaccattg ttgggtatcg
      541 ccattacgag tttgtttggc tacagcctgc aggagttggt tagtcagttt gccatgatta
      601 atcacaaagt tcgactgcac gggcatgaac ctcatacccg tggctcccgt acccgcttgc
      661 attgcagctc tggtaaccgc aggtttaagc gtctgtgcag tcgcctgaac taacgatctg
      721 cctgccattg tcattggact aaacatacca taaaccttta taaattatat ttcgacagta
      781 aaaaaattgc ttcccattaa aaaataactt taaagaataa ttatcttgta tttatatttg
      841 aattatcctc cggccttatc tacaggtagt tgcactacat ttatcatatg ctatcatgaa
      901 tatgcaacac tattcccgta atcttatatc tacgccttat tatttgtatc agaactctcg
      961 gattaattac gtttgagtat atcaacat
//
LOCUS       DBFIFS010000484          986 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.494, whole genome shotgun sequence.
ACCESSION   DBFIFS010000484 DBFIFS010000000
VERSION     DBFIFS010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.494"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..81)
                     /locus_tag="N7A07_005078"
     CDS             complement(<1..81)
                     /locus_tag="N7A07_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479522.1"
                     /translation="MCVTSNVLVMFSSFEIFDVISYKVVFI"
     gene            385..791
                     /gene="ipbA"
                     /locus_tag="N7A07_005079"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             385..791
                     /gene="ipbA"
                     /locus_tag="N7A07_005079"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          320 a          200 c          174 g          292 t
ORIGIN      
        1 aataaaaaca accttataag aaataacatc aaatatctca aaactactaa acatcactaa
       61 aacattagac gtaacacaca tcccccacgc cctgtaattc atagctttta tatttatttt
      121 ttcacatcaa tgtagataaa aacacgcatt aaaaatggct tgtagaaaag cagcacaata
      181 aatagttcat atttagtcta ttgcgtagaa ctactattca aaaaatctta ttaatgagat
      241 aatgagatta catcgataac gttctgattg caggcatact tatctgggtg tgcctctctt
      301 ttttacaact gtacacggtg gcgtatgacg agaaaatatc tcacacagga tgaagtctac
      361 aggctgatgg atgcagccca gagcatgtcc tttcctgaaa gaaatcgctg tctaattatg
      421 atggctttca ttcacggttt tagggccagt gaacttcttg atttacgttt atccgatatt
      481 gatgcatcag gaaaacaact taatattcgg cggattaaaa atggtttctc gacaacacat
      541 cctcttcttc ctgatgaata taatttgatc aagttgtggc ttaagcagag gaagttaatt
      601 gaaaacggag ttgaaggaga ctggcttttt ctctcccgga aacgccgccc tatcagcaga
      661 caacattttt ttctatcatt cgtgaggctg gaagacgtgc aggattagct gtaaaagcac
      721 atcctcatat gttacgtcat gcctgtggtt ttgctctggc tgacaatggt gtcgataccc
      781 gactacttca ggattatctg ggtcatcgaa atatccagca tactgtcaga tacacagcca
      841 gtaatgctgc tcgatttaaa ggggtgtgga aaaaaaagcc tcgttaaaac agtaccttct
      901 gattcaaaat gtcagattcc tacgttttcc tcaattcttc cataaaaaaa cagcccattc
      961 aattcaataa aacacataac atgttg
//
LOCUS       DBFIFS010000485          976 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.495, whole genome shotgun sequence.
ACCESSION   DBFIFS010000485 DBFIFS010000000
VERSION     DBFIFS010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.495"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..145
                     /locus_tag="N7A07_005080"
     CDS             <1..145
                     /locus_tag="N7A07_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGU2479523.1"
                     /translation="LYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIAL
                     PAE"
     gene            463..770
                     /locus_tag="N7A07_005081"
                     /pseudo
     CDS             463..770
                     /locus_tag="N7A07_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          301 a          235 c          192 g          248 t
ORIGIN      
        1 actgtacagc ctgatcggga cgtgcaaact gaatgacgtg gatccagaaa gctaccttcg
       61 ccatgtgctt ggcgtcatag cagactggcc ggtcaaccgg gtcagcgaac tgcttccgtg
      121 gcgcatagca ctgccagctg aataacacat ccccgtcaat acggccctcg ctgtacgctt
      181 accaacaaaa ccccttttaa tcccttatat atcaggcttt ctttgtgtac aggggtcaac
      241 cgactcaacc tgtatcaagt tgcttttgag gatactttag ggggtattat ttcctcttga
      301 aatattagca ccccataatg ggaaaaaggt aatacataac aatggttttt aacagtatct
      361 ttgttataca agggggcatt gaggatatca ggaaaaatct caaaatcggt acggacagga
      421 aactacgtgg gcagcaaaaa cgtatggtaa caccgggaaa tgcaacgccc accagtcggg
      481 ctaatccacc actccaatcg tggttcgcag tactgcacac acgattaccg ggctatacag
      541 gagcggtttg gtctgaaaac atccatgccg cgtaaaggta accgttacga caacacctca
      601 atgaaaaact tatagtaaac gctgaagaat gaaactatcg ttttaataac cgggatgaag
      661 ccatctaagt aatacggaaa tacattgaga ttttctacaa ccatcagcgc cgccactccc
      721 gtctggggaa tatctccctg gtagccttca ggaaaaatat aaccagataa ctgcgtaaaa
      781 aaagaacaaa tggtggtgtc cgctattgcc agtatacctg agtttactgc ctttcagatt
      841 atcacaatca atagcgaaca gacttttaag ctctgtgcgc ttttaacaaa acgtcccttt
      901 ctccactttc accgtataat aatacattat ttattacccc tccaataaaa accgatatta
      961 ctaatcacat ttatta
//
LOCUS       DBFIFS010000486          976 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.496, whole genome shotgun sequence.
ACCESSION   DBFIFS010000486 DBFIFS010000000
VERSION     DBFIFS010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.496"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>976
                     /gene="espK"
                     /locus_tag="N7A07_005082"
     CDS             <1..>976
                     /gene="espK"
                     /locus_tag="N7A07_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGU2479524.1"
                     /translation="IELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATD
                     SIKNVPNGLSSLSINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVT
                     IHAEQKTTWNIGVEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIV
                     YGLSSPREKLVNSIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHE
                     RGIKFKQHLKNHNNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELV
                     NSLKLIANKSEGYGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPA"
BASE COUNT          345 a          209 c          189 g          233 t
ORIGIN      
        1 tattgaatta actgattcac catttataca tttcatatct gaaggcatcg agtgcctgac
       61 ggtttgccac tgctatatat ctggagtgcc agagagtgtc cgctaccttg aaataaaagg
      121 tagcgccaca gacagcataa aaaatgttcc aaacgggtta tcatctctca gcatcaatag
      181 ctataacccg gagaatcagg ccagaattga caacctgata tcaccgtcac tgaagacgct
      241 atcgctgact ggatgtagca atattatact gccggagaaa cttccggaga gtgtgacatc
      301 ggtaaccatt catgcggagc agaaaaccac gtggaacatc ggtgttgaag ggatgcctga
      361 tgggctggat cttgatttac aaaatgtact actctctcca gatgtagtta aagcaaaaaa
      421 catcaccttt cagggcaacg ctctggatgt ggccttacac tttcgcgagg gagacattgt
      481 ctatggacta tcttcaccca gagaaaaact tgtcaacagc attaaactag ttaacgactt
      541 ttccaaaaaa gatattataa ctcagaatac gttaacaaac gcagtatggg accccagaac
      601 acctcgcaaa tataagcaag atccacttat caaaagagca ttaaatgaac acgaaagagg
      661 aataaaattt aaacaacact taaagaatca caataattat aatgttacca tggccgacct
      721 ttccgtatac aatcgcgaca aattatgggc aaaaacaagc aaggccggcc tagagtttca
      781 gacattaaca cgcaataaaa cggttatttt ttgtgcggat gagcttgtca actcactcaa
      841 actcatagct aacaagtcag agggctatgg ccagagtatt accgccagcg aattacgatg
      901 gatttaccgt aataaagaca acaaccaaat aatgaaaaac ataaaatttt atctacatgg
      961 caaagagata ccagca
//
LOCUS       DBFIFS010000487          955 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.497, whole genome shotgun sequence.
ACCESSION   DBFIFS010000487 DBFIFS010000000
VERSION     DBFIFS010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.497"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>955
                     /locus_tag="N7A07_005083"
     CDS             <1..>955
                     /locus_tag="N7A07_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU2479525.1"
                     /translation="QFLEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWD
                     EASASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMA
                     TFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGE
                     RKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWP
                     GDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPI
                     DIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVP"
BASE COUNT          272 a          238 c          238 g          207 t
ORIGIN      
        1 ggcaattcct ggaacattat ccggctgctc aggactgcgc ttataaattt cttgtttgcg
       61 aggataaacc cggtataccc cgccctgccc tcgattcctg ggatgctgaa tatatgcagg
      121 aaaaccgctg ggatgaggcg tctgcttcct ttgtcccggt cgagactgaa tccgatccga
      181 tgaacgtcac ttttgacaac ctggcccctg aagtacagaa cgctgtcatg gttaagttcg
      241 acacatgtga aaacatcact gttgatatgg tgattagcgc acaggaattg ttgcaggaag
      301 acatggcaac attcgacggc catatcgttg aagcgttgat gaaaatgcca gaagttaacg
      361 ccatgtatcc ggagcttaag ctgcatgcca tcgggtgggt taagcataaa tgtaagcctg
      421 gtgccaaatg gcccgaaatt caggcagaga tgcgcatctg gaaaaaacgt cgcgaaggtg
      481 aacgcaagga agccggaaaa tacacgtctg ttgttgatct cgcccgcgcc agagccaatc
      541 aacagcacac tgaaaattca acaggaaaaa tcaacccggt cattgctgcc attcatcgcg
      601 aatacaagca gacatggaaa acactggatg acgaactggc ctacgctctc tggcctggtg
      661 atgtggatgc cggaaacatt gacggcagca tccatcgctg ggcaaaaaat gaagttatcg
      721 acaacggccg cgaagactgg aagcgtatct cggcatcaat gcgcaaacag cctgatgccc
      781 ttcgctacga ccgccagact atttttggcc ttgtccgtga acgtccgatc gacattcaca
      841 aagaccctgt ggcactgaac aaatacatta ctgaatacct gactacaaag ggcgtgtttg
      901 aagatgaagg aacaaatcag agcgcaactg atactctctc gtcgccagta ccaga
//
LOCUS       DBFIFS010000488          932 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.498, whole genome shotgun sequence.
ACCESSION   DBFIFS010000488 DBFIFS010000000
VERSION     DBFIFS010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.498"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..839
                     /gene="yeeO"
                     /locus_tag="N7A07_005084"
     CDS             <1..839
                     /gene="yeeO"
                     /locus_tag="N7A07_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU2479526.1"
                     /translation="WPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYF
                     KPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALIN
                     LPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASF
                     YTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVV
                     VGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAV
                     SE"
BASE COUNT          187 a          183 c          273 g          289 t
ORIGIN      
        1 cctggccggg actgggattt gtcggggctg ggttgggttt aaccatttct cgttatattg
       61 gcgcagttgc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta aggatttcgt
      121 taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg gggattggta
      181 ttccagcgag tgtcgaatca gtgttattta ccagtggtcg gttattaacc caaatgttcg
      241 ttgccggaat ggggaccagt gttattgccg gaaattttat cgcgttttca attgcggctc
      301 ttatcaactt acccggaagt gcgctcggtt ctgcttctac gatcattaca ggccgaaggt
      361 tgggggtagg gcagatagcg caagcagaga ttcagttgcg gcatgtgttt tggctgacca
      421 ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt atggcatcgt
      481 tttacaccca ggatccacag gttaaacatg tcgttgtgat tctgatttgg ctaaatgctt
      541 tatttatgcc tatttggtcc gcttcatggg tgctacccgc tggatttaag ggggctcgtg
      601 acgcccgtta cgctatgtgg gtttctatgt tgagcatgtg gggttgtcgg gttgtcgtcg
      661 gttatgtgct gggcatcatg cttggctggg gtgtggttgg tgtctggatg ggaatgtttg
      721 ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga cgttggctgt
      781 ggaaataccc tcgacccgaa ccgcaaaagt gtgaaaaaaa gccagctgtg tcggaataaa
      841 cgacaaaatg cagattattt cagcaaacga tttcaaattt aaaaaacagg ctttgacatt
      901 gtgggtgggc atcgctaata ttcgcctcgt tc
//
LOCUS       DBFIFS010000489          921 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.499, whole genome shotgun sequence.
ACCESSION   DBFIFS010000489 DBFIFS010000000
VERSION     DBFIFS010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.499"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            180..776
                     /gene="fimE"
                     /locus_tag="N7A07_005085"
     CDS             180..776
                     /gene="fimE"
                     /locus_tag="N7A07_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU2479527.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          264 a          164 c          201 g          292 t
ORIGIN      
        1 atgaaaatag atgttatcac atcagtgata ttttattttt gtatgatatt taatgtaatt
       61 gactgatagc cacatcactt cgtgtgtggt tatcttttta tctattgggc taattttgac
      121 cgattgaggt ttcctatagg tattcattca aatatatctc agttaggagt actactattg
      181 tgagtaaacg tcgttatctt accggtaaag aagttcaggc catgatgcag gcggtttgtt
      241 acggggcaac gggagccaga gattattgtc ttattctgtt ggcatatcgg catgggatgc
      301 gtattagtga actgcttgat ctgcattatc aggatcttga ccttaatgaa ggccgaataa
      361 atattcgccg actgaagaac ggattttcta ccgttcaccc gttacgtttt gatgagcgtg
      421 aagctgtgga acgctggacc ctagaacgtg ctaactggaa aggcgctgac cggaccgacg
      481 ccatatttat ttctcgccgc gggagtcggc tttctcgcca gcaggcctat cgcattattc
      541 gtgatgccgg tattgaagct ggaaccgtaa cgcagactca tcctcatatg ttaaggcatg
      601 cttgcggtta tgaactggcg gagcgtggtg cagatactcg tttaattcag gattatctcg
      661 ggcatcgaaa tattcgccat actgtacgtt ataccgccag taatgctgct cgttttgccg
      721 gattatggga aagaaataat ctcataaacg aaaaattaaa aagagaagaa gtttgattta
      781 acttattgat aataaagtta aaaaacaaat aaatacaaga caattggggc caaactgtcc
      841 atatcataaa taagttacgt attttttctc aagcataaaa attttaaact aactgtttga
      901 tatgtaaatt atttctcttg t
//
LOCUS       DBFIFS010000490          918 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.500, whole genome shotgun sequence.
ACCESSION   DBFIFS010000490 DBFIFS010000000
VERSION     DBFIFS010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.500"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(370..>918)
                     /locus_tag="N7A07_005086"
     CDS             complement(370..>918)
                     /locus_tag="N7A07_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU2479528.1"
                     /translation="PYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHS
                     IKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMA
                     QQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVE
                     PLAIIDRTFGMKENISMVNASK"
BASE COUNT          269 a          198 c          169 g          282 t
ORIGIN      
        1 aaaaaatata ttttcgatat aatcaatgtt atgattaaag agtatttcat cagggcaggt
       61 aaaaacagag taaatcagca aaagaagctg atcttcagcg atactgacac taactgacgg
      121 tttaagcggt cgtatgaagc agcagctttc cgacggactg ccatgcggat cgtttacctt
      181 ttgggctatt ccgcccgtca tcaagcggct cacgagtact gagtttatca ggatgatatt
      241 gctgacaatg gtaattcgtt gaccgatgtg tacttcacta catatcggtc aacaccgtac
      301 tgccgtgcaa catctccaag atgtagcatc gtttttacca gaggaggcag gacatagatt
      361 gtaaaaaaac tatttcgatg catttaccat tgagatattt tccttcatac caaaagttct
      421 atcaattatc gcaagtggtt caacaggtgc ggtcttatct ataacccaac cactctctgt
      481 aacacttcct cgtgtgatat attttgcctt tactttaaca actattagat aatgctcacg
      541 actaaaccga tcagcaacgc taagatccga tgtgtattca ggcaatattc ttccaatacg
      601 tacctgctgt tgtgccatat attcatctgg tgcttttaca tttggatttg aatcaggaag
      661 ccctccagca gttttatatt caacgatatt ttcagcttga ataccggttg tgccgcgaca
      721 caatatgatg tctgaatcgg acagttctat gtgcccctcc ttaattttcg aataaatttg
      781 tttaatacta tgttctggag ttggtactat ttcagaagat aaagcaggca acgtgtcttc
      841 aagcaccgga gaatcatcgt atatatccaa caaatcctca tcatacccaa tatctccttc
      901 tcttaactga gaatacgg
//
LOCUS       DBFIFS010000491          917 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.501, whole genome shotgun sequence.
ACCESSION   DBFIFS010000491 DBFIFS010000000
VERSION     DBFIFS010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.501"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..203)
                     /locus_tag="N7A07_005087"
     CDS             complement(<1..203)
                     /locus_tag="N7A07_005087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479529.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
     gene            complement(361..>917)
                     /locus_tag="N7A07_005088"
     CDS             complement(361..>917)
                     /locus_tag="N7A07_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU2479530.1"
                     /translation="ALNEAVSNGYFDIVKLLVESGADLTIKDEFGKTALELAEVHQNY
                     KIVEWLSHYRDHNPIPDFSVLINIYGERYFGGEIIDTDARTELGEGVLHIAVRKRRQL
                     DVRCFIQHGADINAKANNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGYSPM
                     DLAILMGDKRIIFYLYGYISHVSE"
BASE COUNT          265 a          223 c          153 g          276 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 ttattcagaa acatgagaaa tataaccata taaataaaaa ataattctct tatcccccat
      421 caaaatagcc aaatccatag gggagtatcc catctcactc tccaaataaa tatcagcacc
      481 taatttcaac aaaagcttca tcatatcaaa atcccctatc cctatcgaat aatgcaaagg
      541 agtgaagtca aagccattat ttgccttagc atttatatcg gcaccatgtt gaataaaaca
      601 tctgacatct aattgtcttc ttttcctaac agctatatga agaaccccct cgccaagttc
      661 tgtccttgca tctgtatcta taatctctcc accaaaatat cgctcaccgt agatattaat
      721 aagtacagaa aaatcgggta tgggattatg atctctataa tgtgatagcc attcaactat
      781 tttataattc tggtgtacct cagccaattc taatgccgtt ttcccaaatt catcctttat
      841 agtaagatcg gctcctgatt ctactaaaag cttaacaata tcaaaatacc cattacttac
      901 ggcctcatta agtgcgg
//
LOCUS       DBFIFS010000492          908 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.502, whole genome shotgun sequence.
ACCESSION   DBFIFS010000492 DBFIFS010000000
VERSION     DBFIFS010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.502"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            132..350
                     /gene="ydfC"
                     /locus_tag="N7A07_005089"
     CDS             132..350
                     /gene="ydfC"
                     /locus_tag="N7A07_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU2479531.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
BASE COUNT          245 a          216 c          237 g          210 t
ORIGIN      
        1 gccaccacat tctgggcaag aaaacttgat tgtattcata accaatttcc tctcgagtaa
       61 cagacccctc agaggatacc acctcgcctg acgtggttaa aagcaggcaa cgctaaccac
      121 aaggagccga catgcagaaa cgagaacccg tcatcatcgc gccagactat accgatgatg
      181 aactttatga gtggatgcac cagaaaatta atgcagcgca ggatctgaaa tgggccaatg
      241 aagccagggc taagcaggct gaaaatctgt ccgctctgga gcaggatatc accaatctgg
      301 aaaaagcagc ggcattaagc attgccagaa tgattacata cccgcgttaa tagctaacca
      361 acgaagctaa ggttggtaat taaggagttc tccacgggtg aggtggagtg cgtgcgccgg
      421 acacgggtga gcatacggca ctgacagttt actgaaagga tatttccctg aaaagtcaga
      481 ccataacgcg aaagcgcacg gcgaggtagc tggttcatag atagcctgtc gttaaatttt
      541 cgtcgaccgt gcgcttccgg ttgtggcaat ccgcgaaatg gcgcggcggt aagtatggag
      601 gggttattcc ttcccccgtt gaggacaccg ggttgtcagg ttgaccatac gcttaagtga
      661 caaccccgct gcaacgccct ctgttatcaa ttttctggtg acgtttggcg gtatcagttt
      721 tactccgtga ctgctctgcc gcccttttta aagtgaattt tgtgatgcgg tgaatgcggc
      781 tgagcgcacg cggaacagtt aaaaccaaaa acagtgttat ggggggattc tctgtatccg
      841 gcgttaattg ttaactggtt aacgtcacct ggaggcacca ggcaccgcat cacaaaattc
      901 attgttga
//
LOCUS       DBFIFS010000493          905 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.503, whole genome shotgun sequence.
ACCESSION   DBFIFS010000493 DBFIFS010000000
VERSION     DBFIFS010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.503"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..80)
                     /locus_tag="N7A07_005090"
     CDS             complement(<1..80)
                     /locus_tag="N7A07_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2479532.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
     gene            complement(86..802)
                     /locus_tag="N7A07_005091"
     CDS             complement(86..802)
                     /locus_tag="N7A07_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU2479533.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
BASE COUNT          206 a          263 c          214 g          222 t
ORIGIN      
        1 ctccattccg gcaccttcac cgttttatga cggaaccccg acagtctggc cagcgccagt
       61 gttttcagat cttttgccat aagccttatc cgcccgcacc attaaccgtt actgtacacg
      121 catcagaggt aatgctctgc ggctgttctg cagaatccgt tacctcgcag gtataagccc
      181 ccttatcacc tgactgcgta ttggctttac tgaaagtgtc agtagtctgt ccctctaccg
      241 gctgaccatc cttcttccag gcgtgtttat aaggcggcgt tcccccgttg acactgactg
      301 acattgtcag cagcgcaccg gtattcacgg taagtgtctt ctccagattt ttcacaaacg
      361 ccagcggtac cacataggac accggtttac ccttcaggcg aagtgaaaac gttgcagcca
      421 ccacgccgtt ggtaccggat gaccaggtgt gctgacgcac ttccgccagg aacttaaagc
      481 ccttaccgga cggaaactgc accttaaacg catacaccgt gtcattgtca taggcatcac
      541 gcagggcgtt ctgggcctga ttcagataaa aattacccga catggaaatc tcggacgacg
      601 cccccagacc gttgatgttc tcctgctctg tggagcagag cgtggtcaca tcaatatcct
      661 gtttctgacc ggcggtgaac tggacttcct tgatggtgca gtccaggcgc agatattccg
      721 ccttctccat ggtttcagca gtcgccgggg cagatgaaat catcacctgc gtcagctgtg
      781 aacgttcata caaagcagac attctgcctc ctgataataa aaaacccgca cgcggcgggt
      841 tatgggttct gttgaaaaaa ttacaccgtg acctgaaact ccagggttgc acggtaacag
      901 cggtt
//
LOCUS       DBFIFS010000494          903 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.504, whole genome shotgun sequence.
ACCESSION   DBFIFS010000494 DBFIFS010000000
VERSION     DBFIFS010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.504"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..357
                     /locus_tag="N7A07_005092"
     CDS             <1..357
                     /locus_tag="N7A07_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2479534.1"
                     /translation="AAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGV
                     AQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIST
                     RDEGGDGKVVVIGRQR"
     gene            596..>903
                     /locus_tag="N7A07_005093"
     CDS             596..>903
                     /locus_tag="N7A07_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479535.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     T"
BASE COUNT          229 a          199 c          291 g          184 t
ORIGIN      
        1 gcggccatcg tggggtcgtt cttcactgcc ggggcatcaa tggcgttatg gggttcagcc
       61 ctggcagccg gtggtttttc tgccaccacg atgctgtttt cactgggggc cagcatgata
      121 ctgggtggtg tggctcagat gctggccccg aaggctaaag taccggagta caaaagcacg
      181 gataacggca gacagaacac gtacttttcc tcgctggata acatgattgc acaggggaac
      241 ccgatgccgg tgccttacgg tgaaatgctg gttggctcac ggcgaatctc ccaggacatc
      301 agtacccgtg atgaaggcgg tgacgggaag gtggtggtta tcgggcggca gaggtaaaaa
      361 gaataaaaaa atcccgcagt gaccgaaggc tgcgggaaca gaaaatgaag attaaccaca
      421 gggagttttg tttttattgg cccgaaaaaa ctgtaacgcc cgggaatgat atctgccacg
      481 ggggcgtaca gaaaatgtga agatattcag aatttttatt cagtcatgat acaggcatcc
      541 tccgggatgc ctgttgtttt tgtgcgtaac agttatcaca gtaaagggtg agacaatggg
      601 caaaggtggt ggcaaggcgc acacgccggt tgaggcaaag gacaatctta agtccacgca
      661 gatgatgagc gtgattgatg cgattggtga agggccgatt gaaggtccgg tgaaggggct
      721 gcagagtatc ctggtgaaca aaaccccgct gacggacacg gacggtaatc ctgtgataca
      781 tggtgtgaca gcggtctggc gcgccgggga gcaggagcag acaccacctg aaggctttga
      841 gtcctccgga gctgaaaccg cactgggcgt ggaagtgacg aaggcaaagc cggtgacgcg
      901 cac
//
LOCUS       DBFIFS010000495          877 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.505, whole genome shotgun sequence.
ACCESSION   DBFIFS010000495 DBFIFS010000000
VERSION     DBFIFS010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.505"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..268
                     /locus_tag="N7A07_005094"
     CDS             <1..268
                     /locus_tag="N7A07_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2479536.1"
                     /translation="YYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSDTNPTLSE
                     YYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          289 a          145 c          179 g          264 t
ORIGIN      
        1 atattactgc tttactaaaa ataacattta caaacaattg cgatacttag aaaaatcagc
       61 atctcagggg aatgttacag cgcagtttaa ttatggggtc tttttatcag atacaaatcc
      121 aacattatca gaatattata atttgaatag agctatttat tggatggaat tcgctgtaaa
      181 taacggtaat attgatgcaa agagcaaact tcaagagctc aaaaagctaa agcgaatgga
      241 tagaaggaag aataaggaga atccgtaaaa gcaatagttc atcagactgc cgcagcagta
      301 gtatgatgat gaatcagggc tgtactataa ctattatcgg taacattaac cgcagcaggg
      361 gcggtataac actcaggctc cgattgggct ggaaggtagg tggaattttt atcagtatcc
      421 gttgaacccg atttctggaa tcgatcccct tgggattagc cacctgttta tacagcataa
      481 ctttaggtat gctttcgtgt gtttcagata taccatgtga tgataatttt tatgatgtgc
      541 ttagtattcc agtcgcttct ggtaataata tgcaatgtaa aaataatccc cgttgtacac
      601 atttataaat agatggccaa tatctcaagg cctatggagt tggaatgtta atgacccggg
      661 agcaagcaat agaaaacctg acggcatacg attagtaccg tcagttaata caggaactta
      721 taatcgcaat ggattttcaa tacatagttg tttaaacgct tttggtccgt ctttaggacc
      781 tcgattctgt tccgagggtt gtataactgg tttatctaat gacatgcaaa aattaaatga
      841 gttaatattt tcagagccag atagcacatt aacagtt
//
LOCUS       DBFIFS010000496          877 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.506, whole genome shotgun sequence.
ACCESSION   DBFIFS010000496 DBFIFS010000000
VERSION     DBFIFS010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.506"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..554)
                     /locus_tag="N7A07_005095"
     CDS             complement(<1..554)
                     /locus_tag="N7A07_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU2479537.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPR"
BASE COUNT          264 a          143 c          158 g          312 t
ORIGIN      
        1 attcttggct gctcattggt ccatcttttt gcctgagaag ctggaagggt tgaaatatat
       61 cctatcgtgg ttccggcgta acctggaaca ttgcaaccag ggcctaatgt tatttcatga
      121 actgtatagt cctcaacagg atcgaaaggg ctcttcataa ataaatttag ccgtaaacct
      181 tctgctaact ctgtagcagc atgcgccttt ttacatatac caccatatat ttcgtctaaa
      241 gctgaggtga ttttcaagtt tcgatagagc atacgtcctg ttgttgtaat accatcggat
      301 tctctgcatg cttctgtcac ataattaaat ggccaggatt ctttactaat atcaagtttt
      361 ctaatttcga caacgctttt tccatcgtaa ttatggttat tgccacgagc aagaggcaga
      421 agatagatat tttccgtatc caggcgtgca cgttcatatt tacgtgttaa ctcactttcg
      481 acttttggat gatgaaggaa gtgattatct gtaataattt gactctgaag gacattgctt
      541 aatggcatag acataatagt taccttttat gagtatttca ctgatgttta gaaaaataga
      601 taaatttttc tgtagtaaaa agagaagtaa acaaatgaca tgcatgtttc tgttgtagtg
      661 atatcaactc tacacagtga tattaaaggg taggaaacac tctaaagtat caaaaaacgc
      721 tcatttaaaa ttatttgcat gcaatttaaa atcatatctt attactaatt ggaatttgat
      781 gttgctatat tgaggtctat attaataatg cctgtgaatg gtgtttttga tgtatttgat
      841 atgttatcaa tttatattat ttacaaacta attgttt
//
LOCUS       DBFIFS010000497          859 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.507, whole genome shotgun sequence.
ACCESSION   DBFIFS010000497 DBFIFS010000000
VERSION     DBFIFS010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.507"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>859
                     /locus_tag="N7A07_005096"
     CDS             <1..>859
                     /locus_tag="N7A07_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2479538.1"
                     /translation="LNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQYRSYDS
                     NNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQILSTVQ
                     IGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVSQNGYII
                     YQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPGISNFDF
                     IAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNTPLGAIS
                     FDATRSSSK"
BASE COUNT          248 a          180 c          179 g          252 t
ORIGIN      
        1 gactaaattt aacagtacca caggcgtata tcaatgaact tgaaagaggt tacgttcccc
       61 cggaaagttg ggatcgcggc attgatgctt tttatacctc ctataattta agtcagtatc
      121 gctcttatga cagcaataat aacagtaata cagccagtta tggccgattt aacagtggat
      181 taaatttatt tagttggcag ttacactctg atgccagcta tagcaaaccc gatgatatga
      241 aaggcacatg gcaaagtaat actctttacc tggaacatgg ctggtcgcag atattaagca
      301 cagttcagat aggagaaaat tacacatcct cactcatatt cgactctctg agatttagcg
      361 gaatacgctt atttcgcgat atgcaaatgc tacctgattc tatgcagagc tttacacctt
      421 tagtacaagg tgttgcgcaa agtaatgcct tgataacggt ttcgcaaaat ggctacatca
      481 tttaccaaaa agaggttccc ccgggaccat ttactatcgc agatttgcaa ctctcgggca
      541 gcggctctga tctcgatgtc agcataaagg aagcagatgg cagtgttcgt tcctttttgg
      601 tgccctactc ttccgtgcct aacatgctgc aacctggtat ttcaaatttc gattttatcg
      661 ccggacgcag taagatatat ggtgtaaaga atcaggagga ctttctcgag gcaaattata
      721 tatatggcct caacaatctt ttgacacttt atggcggtac gattttatcc gataactata
      781 atgctataac tctgggaaat ggttggaata caccgctggg tgccatctct tttgacgcta
      841 cacgatcgag tagtaagtt
//
LOCUS       DBFIFS010000498          856 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.508, whole genome shotgun sequence.
ACCESSION   DBFIFS010000498 DBFIFS010000000
VERSION     DBFIFS010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.508"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            177..779
                     /gene="lpfA2"
                     /locus_tag="N7A07_005097"
     CDS             177..779
                     /gene="lpfA2"
                     /locus_tag="N7A07_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HGU2479539.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          257 a          165 c          177 g          257 t
ORIGIN      
        1 aatttagttt tgcttaacac ctaaattaat ttatctaaat gtataaaata aaaaccactt
       61 gcgccaaagt atattggtag tactatttgg tttctgatca aataccgatc aggtttatcc
      121 aaatttaata aatggataac acacaggaat aatatcaaat taatggagtt atatttatga
      181 aaccaaatat gattgtagga gcattagcgt taacttctgt gtttatggca ggtcacctac
      241 aggcggctga tggaacagtc catttccgtg gtgaaattat tgacagtact tgcgaagtca
      301 ctcctgaaac taaagatcag gtcgttgatt taggcaaagt aaaccgtaca gcctttagtg
      361 gcgtcgatga tgtggctgcc ccgacggctt tttctatcga tctgactcaa tgcccggaaa
      421 cctttaagtc cgccgcaatt cgtttcgatg gtaatgaaga tgctcatggt aatggcaacc
      481 tggcaattgg taccccgctg gataactcta acgatgctgc cgctggtatt agcccgagtg
      541 ataacagtgg ggattatact ggtgcgggtg ccgttagtgc agcgaaaggc gtagctattc
      601 gtttatataa ccgtgcagat aacactcagg tcaagttata tgaaaattct gcatcaactc
      661 cgatttctaa tggtaatgca tccatgaagt tcatggctcg ttatattgct acggaaacga
      721 ctattgaccc tggtacagct aacgccgact cgcagtttac agttgaatat ataaaataaa
      781 tcattcttaa cataatacct taaataaaat ggcgctcgcc attatggttc tcttttactt
      841 aaatgaaggg cgtcat
//
LOCUS       DBFIFS010000499          843 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.509, whole genome shotgun sequence.
ACCESSION   DBFIFS010000499 DBFIFS010000000
VERSION     DBFIFS010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.509"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(5..571)
                     /locus_tag="N7A07_005098"
     CDS             complement(5..571)
                     /locus_tag="N7A07_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2479540.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          272 a          186 c          134 g          251 t
ORIGIN      
        1 acatttacat ttccttcgat agattcgcat aagtgctcct ttcgttgccg gaaaaatcac
       61 cgtaatactt atctcgggct tcttcagcaa ctagtaccgc taactccaga tcatcaaagc
      121 atccgaagtg tttactcttg ccatggaatc ctagcctaac attccatttt ttctgtcgtt
      181 tgtgccaagt aacccctctg caacctgatt tgctattctt tcggatcctt atatttcttg
      241 aattttctat tggcaggcat tctcttaaat tttctggcct attgtcggtc ctaattccat
      301 taacgtggtc aatttgacca gcaggccaac gattatgagt tatgtaaaaa actaagacgt
      361 gagttttata tctacgccca tctatcatga tcattgaata accgttggaa tcaaaagctc
      421 cagcaacact atttaatgct atccttccct gagtgggaac tttccatcta aataccccgg
      481 tagatttatc gaaacttagt aactcaaata tccttttaac agttaaatct tctcttttac
      541 ggttacatcg tcttcgcgct ggtttagcca tcgccttctt cctccgtaat ggttttctga
      601 atttggccac ctgaacagag ctcaccaaag ctatggatgt cggtatttcc acaataccaa
      661 gatggcgaaa ataactgcat gataagcctc agggaaaagg gaagacacta cccccgataa
      721 ttcagaaaca aatcgaaata catgaactaa agaaaaaatc acaacaaata gaaatagaaa
      781 tagaaataga aatagaaata gaaatagaaa tagaaataga aatagaaata ttaaaacaga
      841 cca
//
LOCUS       DBFIFS010000500          834 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.510, whole genome shotgun sequence.
ACCESSION   DBFIFS010000500 DBFIFS010000000
VERSION     DBFIFS010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.510"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..526)
                     /locus_tag="N7A07_005099"
     CDS             complement(<1..526)
                     /locus_tag="N7A07_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2479541.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQE"
     gene            complement(640..>834)
                     /locus_tag="N7A07_005100"
     CDS             complement(640..>834)
                     /locus_tag="N7A07_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2479542.1"
                     /translation="LTAQLRLGPADILESDENGIIPEQDRVITQVVILDADKKQIQCV
                     VRPLQILRADGTWENIGGMK"
BASE COUNT          229 a          228 c          158 g          219 t
ORIGIN      
        1 gttcttgact tacaatcatt gatgacgtta ttggttctcg tgttagcggg tgcttcccac
       61 cgtcgcgaac caaatgagaa aaagaaaccg aatcaaataa ggtacaaacc aaagaacctt
      121 ctgaattttt aacaaacaca ccttcttctg gtttatccag tataattggg catcgaaccg
      181 cctcggatgg acactgaagg ctctccgtat taacagaaaa cttacattgt gatattttcc
      241 cctcaatatc accatgtgat tgtgtgttac ctgaacctga aaccattctt aacattatat
      301 ccctgagcgc ttcaggacca ccattaagcc ctgtaagtaa taatccagaa agcaaactgt
      361 tattgccccc actcgccaga aagcgaccat ccgtttcact atatgtgata cggaccgtct
      421 gcccgcctac tgtaatttct ccaccgccac tggcggccat ttgagcacga agaacctgca
      481 ttccggtgga aaatgaattg gatgcaatag ctgatgttaa cggcatgaca aaactcccta
      541 tttaaatatt cattccaaac acagggagtc ataacaaaca gcccccatac atgacaacaa
      601 aaaccggagc cggactccgg tttttgtgaa gctgtcgggc tatttcatcc cgccaatatt
      661 ttcccacgtc ccgtcagcac gcaggatttg cagcggtctt accacgcact gtatctgctt
      721 tttatccgca tccagtatca ccacctgcgt gattaccctg tcctgctccg ggataatacc
      781 attctcatcg gactccagaa tgtctgccgg tcccagtcgc agctgtgctg taag
//
LOCUS       DBFIFS010000501          832 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.511, whole genome shotgun sequence.
ACCESSION   DBFIFS010000501 DBFIFS010000000
VERSION     DBFIFS010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.511"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(65..250)
                     /locus_tag="N7A07_005101"
     CDS             complement(65..250)
                     /locus_tag="N7A07_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2479543.1"
                     /translation="MSSKNRPRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            292..657
                     /locus_tag="N7A07_005102"
     CDS             292..657
                     /locus_tag="N7A07_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2479544.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
BASE COUNT          186 a          195 c          231 g          220 t
ORIGIN      
        1 gtactgccgg gggacgaatg gaaaactgat gtgtccggta actgtgtgtt ctgtgaacac
       61 catgttactt aattatgtaa ttcatacccg aacgctctgt tgacagcctt cttctgcagg
      121 cttcaataac ccacgctgaa aagttaccgg aacctttatg ttcaagggcg atattgatct
      181 gttcaatcat gtgattgggg aaacggatat tgcggattgt ggttctgcgg ggccggtttt
      241 tcgatgacat tttctttcct ctggtgacaa gctatatggc gaggatttta catggctgtg
      301 cttcgtacgt taccgggcag aatcaaaact ctgaacaccc ggcggataaa tgtcctgagg
      361 ggggaacagc gtcgtgtcag tggcagtgcc cgggtttccc tcaaacgtcg tatctggcgg
      421 agggatgccg gacactgttg tctctgtaga cgcgtggttg acctttgtga cagtgaactc
      481 gatcaccgca ttgcacttca gttcggtggt ggtaatgagg agacgaacct ctggaccctc
      541 tgtactgaat gccatcgcca gaagtcagcg agtgaagcgg cgagtggtat gcctgatccg
      601 acgttgcctg agcttcctga tggcacgctc agggccgacg gaatcactgg cctgtgacca
      661 gacccggggg ggatcatccg gcgaaaaaaa acgatcgccc tggacaccgc gccccctctc
      721 acgcagagaa aaaattcccg tttcagggca gttaacatgt taactggctg cccgggcatt
      781 tttgcggttt ttatctttat tattcagttt gttgtgcgga aaaaatgtta ac
//
LOCUS       DBFIFS010000502          829 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.512, whole genome shotgun sequence.
ACCESSION   DBFIFS010000502 DBFIFS010000000
VERSION     DBFIFS010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.512"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>829
                     /locus_tag="N7A07_005103"
     CDS             <1..>829
                     /locus_tag="N7A07_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2479545.1"
                     /translation="REKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTV
                     SKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPL
                     SNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLT
                     PDGRLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRT
                     STYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNG
                     "
BASE COUNT          239 a          198 c          232 g          160 t
ORIGIN      
        1 tccgggaaaa aataggaaaa ctgcatctgg ctcaggggct atgggagctg atagacaaca
       61 gccagcttgc ggatgagatg gcggagatga agaccaccat cacggaaacc cgcaatgaaa
      121 tcacacagac ggtcagtaaa acgctggaga accagagcgc cactatacag cagatacagc
      181 gcgtgcagaa ggacacaaat gatgacctgg ctgcgctgta catgctgaag gttcaaaaaa
      241 cgaaagacgg cattccctat gtggccggga ttggtgcagg gattgaggat actgatggcc
      301 agccactgag caacatactg ctgctggctg accgtatcgc gatgataaat ccggagagcg
      361 gcaacagcac gccgttattt gtggcgcagg ggaatcagct gttcatgaac gacgtgttcc
      421 tgaagcgact gtttgcggtg agtatcacct cgtccggcaa tcccccgacg ttttccctga
      481 cgccggacgg gcgactgacg gcgaaaaatg cggatatcag tggcagtgtg aatgcgaact
      541 cagggacgct caacaacgtc acgattaatg agaactgtca gattaagggg aaactgtcag
      601 ccaaccagat tgaaggcgat attgtcaaaa cggtcagcaa gtctttcccc cgcacgagca
      661 cttatgccag tggcaccatc acggtaagaa tcagtgatga tcagaagttt gaccggcagg
      721 tcatgatacc gccagtgtta ttccgcggtg gtaagcatga gaatttcaac agtaataacc
      781 aacagtcata ctggtattca acctgccggt taagagtgac ccgcaatgg
//
LOCUS       DBFIFS010000503          825 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.513, whole genome shotgun sequence.
ACCESSION   DBFIFS010000503 DBFIFS010000000
VERSION     DBFIFS010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.513"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..268
                     /locus_tag="N7A07_005104"
     CDS             <1..268
                     /locus_tag="N7A07_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479546.1"
                     /translation="TRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQ
                     SSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            265..>825
                     /locus_tag="N7A07_005105"
     CDS             265..>825
                     /locus_tag="N7A07_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU2479547.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          203 a          208 c          233 g          181 t
ORIGIN      
        1 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
       61 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
      121 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
      181 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
      241 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
      301 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
      361 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
      421 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
      481 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
      541 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
      601 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
      661 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
      721 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
      781 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcg
//
LOCUS       DBFIFS010000504          807 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.514, whole genome shotgun sequence.
ACCESSION   DBFIFS010000504 DBFIFS010000000
VERSION     DBFIFS010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.514"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            1..331
                     /locus_tag="N7A07_005106"
                     /pseudo
     CDS             1..331
                     /locus_tag="N7A07_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            382..684
                     /gene="bamE"
                     /locus_tag="N7A07_005107"
     CDS             382..684
                     /gene="bamE"
                     /locus_tag="N7A07_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU2479548.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
BASE COUNT          267 a          179 c          170 g          191 t
ORIGIN      
        1 aaagctggag atgaaatggt ctccagagca aatatcagga tggttaaggc gaacaaaacc
       61 acgtcaaaaa acgctgcgaa tatcacctga gacaatttat aaaacgctgt actttcgtag
      121 ccgtgaagcg ctacaccacc tgaatataca gcatctgcga cggtcgcata gccttcgcca
      181 tggcaggcgt catacccgca aaggcgaaag aggtacgatt aacatagtga acggaacacc
      241 aattcacgaa cgttcccgaa atatcgataa cagacgctct ctagggcatt gggagggcga
      301 tttagtctca ggtacaaaaa actctcatat agattcgttt ttctgacctt ggcaggttgt
      361 tcttcagtag gcaaccagtc attgaaaaat gagacgcagg aaagtgtgaa aaccaaaatt
      421 gttaaaggca aaactacaaa acaggacgtg ttagcatcgt tcggtgaacc tgacagccgt
      481 tctttgatcg atggtgaaga acaatggtca tacactatgt ataacagcca gtccaaagca
      541 acctctttca tccccgttgt gggactgctt gcaggtggcg cagactcaca aactaaatct
      601 ctgacagttt ctttcaaagg cgaaaaagtc agcacataca tctttaatgc tggaacaagc
      661 aacgtgaaga ctggcatttt ttagataaaa gacaaaacca gcatcaaggg aaaatttagc
      721 ccgcagttga tacctacggg ctttttacag aaacataaat cactttttaa agaaagtgat
      781 tatctttttc agcctccccc aaacttt
//
LOCUS       DBFIFS010000505          806 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.515, whole genome shotgun sequence.
ACCESSION   DBFIFS010000505 DBFIFS010000000
VERSION     DBFIFS010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.515"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>806
                     /locus_tag="N7A07_005108"
     CDS             <1..>806
                     /locus_tag="N7A07_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2479549.1"
                     /translation="TTLLVESLDRLSREKVGEAIERLKLILNHGIDVITLCDNTVYNI
                     DSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKRQDALESGTIMTASCPRWLSL
                     DDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLNDHAVKNFSGKESAWGPSVIE
                     KLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDLFYAVQEIRLAPFGISNSSKN
                     PMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMRRLHRCDRPSIKR"
BASE COUNT          266 a          125 c          187 g          228 t
ORIGIN      
        1 aactacattg ttagttgaaa gtctggacag actttcaaga gaaaaagtcg gtgaagcgat
       61 tgaacgtctg aaattgattt tgaatcacgg tattgatgtt ataactcttt gcgacaatac
      121 agtctataat attgactctt tgaatgagcc atattcatta ataaaagcca tacttatagc
      181 acaaagggca aatgaagaaa gcgagataaa gtcaagtcgg gttaaattat catggaagaa
      241 aaaacggcag gatgcactgg aatcaggtac gattatgacg gcgtcttgtc cgagatggct
      301 ctccttagat gacaaaagaa cggcttttgt tccagacccc gacagggtga aaactattga
      361 gctaattttt aaactcagga tggaaaggcg ctcattgaat gcaatagcca agtatttaaa
      421 tgatcatgct gtaaagaatt tctcaggaaa agaaagtgca tgggggcctt ctgtaattga
      481 aaaattatta gcgaataaag ctctgatagg tatatgcgta ccttcatatc gtgcaagagg
      541 gaaagggata agtgaaatcg ctggctatta tcccagagtc atatcagatg atttgtttta
      601 cgctgtacag gaaattcggt tggcaccttt tggtattagc aatagtagca agaatcctat
      661 gctaataaat ctacttcgaa cagttatgaa gtgtgaggct tgtggtaata ccatgattgt
      721 tcatgcggta tctggaagtt tgcatggcta ttatgtttgt ccgatgagaa gattacatcg
      781 atgtgacagg ccatcaataa agagag
//
LOCUS       DBFIFS010000506          798 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.516, whole genome shotgun sequence.
ACCESSION   DBFIFS010000506 DBFIFS010000000
VERSION     DBFIFS010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.516"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            8..214
                     /locus_tag="N7A07_005109"
     CDS             8..214
                     /locus_tag="N7A07_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU2479550.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            279..503
                     /locus_tag="N7A07_005110"
     CDS             279..503
                     /locus_tag="N7A07_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479551.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          191 a          184 c          207 g          216 t
ORIGIN      
        1 ttagccgatg cgagagctga aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag
       61 cgcctgcgga tcaacgccac ctgccccggt accgtgcgtg aagccaccgg cacctccggc
      121 gtgggcaatg atgccgccgt cgaactctct ccggttgctg gacgaaacgt tctcggtatc
      181 agagacggaa tcatcagcga ccaggcagca ttgagaatgc ttcaggaata tatccgcact
      241 cagtgtatta actagtattt ttgttattcg gagaatgcat gaagaaatta ttggtaaccg
      301 taaagccttt tcagggaaca attccgttcc gtattttgca gcgtggtcgt gttcttgttg
      361 aaggttcgtt cagtggtaaa tgtacgcaat tacactccag gacctttcag gtgaatgcca
      421 cgaatgaaga gctaaccgtt gagtgtacga tgaatgccgc taaatgccgc atggtatccg
      481 ctgcattaca gccagtgtgt tgagcgacct tattattcat gcgcggtatt gtcgccgtat
      541 tcctgcatta acagagaccg cagcccgacc gggagaatcc tctgcgcgag tgtgcgggga
      601 taatcaaaaa cgatacacac cggggtttac cgcgttaacg gagcgcggcg ttgtcccctc
      661 atggtcgctg gtccggtgcg atggtggaag aagccggatg tttatcacta ttaattgata
      721 acacagaaat ggattcattg attttcagca cgtttttgta ttcgtattat tgaacatctg
      781 tttattttac ttttaaca
//
LOCUS       DBFIFS010000507          786 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.517, whole genome shotgun sequence.
ACCESSION   DBFIFS010000507 DBFIFS010000000
VERSION     DBFIFS010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.517"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..523
                     /locus_tag="N7A07_005111"
     CDS             <1..523
                     /locus_tag="N7A07_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HGU2479552.1"
                     /translation="VPPTAYGKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTS
                     CIFQESCKDNLVVESNGDIYECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAK
                     CQQCAYKPICNGGCPKHRITKVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPL
                     YHIMDVAKQMENN"
     gene            complement(489..611)
                     /locus_tag="N7A07_005112"
     CDS             complement(489..611)
                     /locus_tag="N7A07_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479553.1"
                     /translation="MTKHSTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          247 a          151 c          142 g          246 t
ORIGIN      
        1 tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa
       61 cgatgtgggt gaaattttca tccgtcagtt tgaaagcttt gtcagccgtt ttttggggaa
      121 tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
      181 tggagatatt tacgaatgcg atcattttgt ctatccacag tacaaacttg gaaacattaa
      241 taaatctgaa cttaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
      301 agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgcc ctaagcatcg
      361 tattactaaa gtaaacgatg agactgtttc ctatttttgc gaaggttata aaatcctttt
      421 ttcaaccatg gtaccttata tgaacgccat ggtggagtta gccaagaaca gagtaccgct
      481 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct ctcttttagc
      541 tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat tcctgtcgag
      601 tgtttcgtca tcatcatctt cccttgcgaa aatcttggct tacgtggtat gcgtcagggc
      661 attagcaata cccttgatta tcaatagcat aaccataacc actgaaaatt aaaaatttct
      721 atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc attgccatag
      781 ataatc
//
LOCUS       DBFIFS010000508          779 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.518, whole genome shotgun sequence.
ACCESSION   DBFIFS010000508 DBFIFS010000000
VERSION     DBFIFS010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.518"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(582..>779)
                     /locus_tag="N7A07_005113"
     CDS             complement(582..>779)
                     /locus_tag="N7A07_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HGU2479554.1"
                     /translation="LLIVNNKILNLPIRKLFPTPIASSYGMNFEFMPELKWSFGYPGA
                     IIFMILAGLAPYLYFKRKNWL"
BASE COUNT          267 a          146 c          121 g          245 t
ORIGIN      
        1 ttccaattat atttaattaa aatatacccc atcaataaaa acttacacac tctaaattac
       61 tactgtatag ttatcaatgc aaagactgct atattgcata tatatatgca aaccacataa
      121 ttcaggtaat atatattcaa aacaattata tatataaaac aatgtaaaaa atgttcaaat
      181 catccatcag aaatatattc tacccatcca agcttgagcg tttatcacgc tttcagttca
      241 taatctatac attaacatca tggtcaatat taataacttt gaatgatatc aacctggctt
      301 tgagttttat tctggttatt aataatacct atgtaaatat caatattgga gcaaaaagaa
      361 taagagatac tggatgtaag gcaatctact ttgtattgat ttcattgggt ttgacacttt
      421 tttctattat ctctgcagca attgaagggg tgagatgtga gtcattaagc gtccaactca
      481 acgtggcatg gatattattc acaacaatta tgttatctgc gattccctct gctaaaatgt
      541 cgctgtcagc acactaactt actcacagcg acatcgtata gttacagcca gttcttgcgc
      601 ttaaagtaca gatacggtgc caggcccgcg aggatcataa agataatcgc gcctgggtag
      661 ccgaaactcc acttcagttc tggcataaac tcaaagttca tgccatagct ggaggcaatc
      721 ggcgtaggaa aaagtttcct aatcggtaaa ttcaatattt tgttattaac aatgagcaa
//
LOCUS       DBFIFS010000509          777 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.519, whole genome shotgun sequence.
ACCESSION   DBFIFS010000509 DBFIFS010000000
VERSION     DBFIFS010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.519"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(190..678)
                     /locus_tag="N7A07_005114"
     CDS             complement(190..678)
                     /locus_tag="N7A07_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2479555.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(669..>777)
                     /locus_tag="N7A07_005115"
     CDS             complement(669..>777)
                     /locus_tag="N7A07_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479556.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT          200 a          193 c          164 g          220 t
ORIGIN      
        1 attgcctgat tttgatatgt tcaatccagc atcaaatgaa ggttaattta tggacgaaaa
       61 acagttacag gctctaatga cctttatacg attgacccaa ataaaaaaag ccaccgttgc
      121 aacttaagag tcactaacgg cagcttatgc gaatagtgtt gctcatttgc tcaacgatgt
      181 caacacgttc tatgctacat gcttaatttt ctctacacgt ttccggtttt taaacgcact
      241 atccagaatc gggtaaatca taaacaacga ggcattgagg atttcgtcaa cttcccgtcg
      301 gcaggttgct agcgatggtt tttgaatgcg cccgccgccc cggcataaca tcttgcgagg
      361 tcttgcgacg ctatgatagt aagatgcaat ggcgtgcttg gaagagccgt gggcgtagta
      421 gctgaggagg atgccaaagg ctttcttgtc aatgtacatg acggaatcga cgacctgaga
      481 aatcaacatt ccatcatcat cattacacat tggccttgtc ataactcttc ccggctccac
      541 gctctccatg aatttagcta ttacgctgct catgcgcttt tccagacgac ctgaataaac
      601 ccatgcgccc cacagttcaa gccagccatt cagccactca tgctgttctt tggtgaggtt
      661 tagttctctt atgcccacgc gccttctccc tgtacctgaa tcaatgtgag atttccgcag
      721 aacactgcgc cggtatcgat atacatctgg ttggcaaatt tgagtggttt cactgct
//
LOCUS       DBFIFS010000510          767 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.520, whole genome shotgun sequence.
ACCESSION   DBFIFS010000510 DBFIFS010000000
VERSION     DBFIFS010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.520"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>767
                     /locus_tag="N7A07_005116"
     CDS             <1..>767
                     /locus_tag="N7A07_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HGU2479557.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEW
                     CAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLY"
BASE COUNT          175 a          219 c          252 g          121 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cgaccatcgc ggcctgccgc tggcccttat cagtaaggaa ggggcaacag aatggtgcgc
      661 agaatacgat gagtggggca acctgctgaa tgaagagaac ccgcatcagc tgcagcagct
      721 tatccgcctg ccggggcagc agtatgatga ggagtccggc ctgtatt
//
LOCUS       DBFIFS010000511          765 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.521, whole genome shotgun sequence.
ACCESSION   DBFIFS010000511 DBFIFS010000000
VERSION     DBFIFS010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.521"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..735
                     /locus_tag="N7A07_005117"
     CDS             <1..735
                     /locus_tag="N7A07_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479558.1"
                     /translation="SSIDEKKKKAQVTQSNINKNTPQQLTDKDLFGNETTLAVSEEDI
                     QAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRKYYTVSTFSGIPDRQKPLTCNK
                     NKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIVYQDMLQTGKYDSALKSTVWSDY
                     KNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYESRVIFPPAGITKTSNKELSNDH
                     VTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          248 a          155 c          171 g          191 t
ORIGIN      
        1 tcttctattg atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac
       61 actccccagc aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc
      121 gaagaggata ttcaagcagc gcttgatgga gatgagttcc gcgttcccct taattctcct
      181 gtaatacttg ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga
      241 aaatattata ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt
      301 aacaaaaaca aagataaaaa tgagaacgag gatgttgccc gtgctgagaa tatgaactgg
      361 atgcaggcac tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat tgtttaccag
      421 gatatgttgc agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat
      481 aaaaatgaaa aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg
      541 gatgtggcaa caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt
      601 tttccgccgg ctggaataac aaaaacgagc aataaagaac tatcaaatga ccatgttacg
      661 gaagcgcaac tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg
      721 agtcgattcc agtaatcgtt attgttatgc tgccgcctgt agttt
//
LOCUS       DBFIFS010000512          759 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.522, whole genome shotgun sequence.
ACCESSION   DBFIFS010000512 DBFIFS010000000
VERSION     DBFIFS010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.522"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..282)
                     /locus_tag="N7A07_005118"
     CDS             complement(<1..282)
                     /locus_tag="N7A07_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2479559.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISRE"
     gene            344..759
                     /locus_tag="N7A07_005119"
                     /pseudo
     CDS             344..759
                     /locus_tag="N7A07_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          205 a          193 c          178 g          183 t
ORIGIN      
        1 ttcacgtgag atcgtcgaag gactgcgatt cagcgcagta gctatcgcac gaatgctcat
       61 tttggctgac aaaccagctc gtatctcctc gcgctcagac agtgtcaggt gagctacagc
      121 ccgcttacgc tcatggggtt ttatgccgcc agtatccctt aacatagtga agatcgttcc
      181 gggttttgaa cccaggatat tcgctatttc actgaagcct gttccgttct tccatagttc
      241 aaaaacagag gctttttcct ctgctgtaaa tgttcgtctc attcaaaaaa cctccgcaac
      301 cccatgtttt cacataactg ttgcgttgac caattgaatc tacaacagcg tattttgcac
      361 aggagtcgct gaaaaatacg cgttaatcga acaatggcga caacaatttc ccattgaagc
      421 gatgtgtcag gtatttggtg tatccaggag cggttattac aactgggtac agcatgaacc
      481 ctcagacaga aaacaaagtg atgagcggct aaaactggag attaaggtgg cacatatccg
      541 cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagaga atggcatcat
      601 cgttggtcgt gaccgactgg cacgtcttcg taaggagcta aggctacgct gtaagcagaa
      661 acgcaagttc agggcgacta cgaactcgaa ccacaatctg ccagttgcgc caaatctgct
      721 gaaccagacg ttcgctccta cagcaccaaa tcaggtctg
//
LOCUS       DBFIFS010000513          752 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.523, whole genome shotgun sequence.
ACCESSION   DBFIFS010000513 DBFIFS010000000
VERSION     DBFIFS010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.523"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>752
                     /locus_tag="N7A07_005120"
     CDS             <1..>752
                     /locus_tag="N7A07_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2479560.1"
                     /translation="FGNGEKLKVKIESLGKTSDTIGKSSNAQAVLPYVVKIARGTPDF
                     TGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLTSKLAGDTFTLGL
                     NISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLKCDNLFGDKKQAS
                     RNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKISANGDQGAATS
                     LWKTDKPGVSLNSNIINIPVMASYYVYDEKKV"
BASE COUNT          253 a          121 c          171 g          207 t
ORIGIN      
        1 tttggtaatg gcgaaaaatt aaaggttaaa atagagtctt taggtaaaac cagcgacaca
       61 attgggaaat ccagcaatgc gcaggcagta ttaccttatg tggttaaaat agccagaggc
      121 acacctgatt ttactggaga aagaaaatct acctggttta tttcagatac cgtgattgca
      181 aatattggcg gtgagtcatc gtcatccatc gatttttggt tgggtatttg taaggcattg
      241 aagtttaact ggtgtgtgaa ttatctcacc agcaaactgg cgggggatac atttacgctt
      301 gggttaaata tttcctatta tcctaaaaat acgacctgta agcctgaaaa caccgttata
      361 aaagtagatg atatcgcctt gttccagctc agaaatcagg gaaagattgc ggcgaacagt
      421 aaggaaggaa caattacgtt gaaatgtgat aatcttttcg gcgacaaaaa acaagcatcg
      481 cggaatatgg ttgtatatct ttctagcagt gacttagtta aaggaagtaa tactattttg
      541 cgtggtaaaa cagataatgg tgtagggttt gtgttggatc taacagaacc accaaaaggg
      601 actgaggctg ccattaaaat ttcggccaac ggcgatcagg gcgcggcgac atcattatgg
      661 aaaacagata aaccaggagt ttcattaaat agcaacatta ttaatatacc agtcatggcc
      721 agttactatg tatatgatga aaaaaaagtt aa
//
LOCUS       DBFIFS010000514          748 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.524, whole genome shotgun sequence.
ACCESSION   DBFIFS010000514 DBFIFS010000000
VERSION     DBFIFS010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.524"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..485)
                     /locus_tag="N7A07_005121"
     CDS             complement(<1..485)
                     /locus_tag="N7A07_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2479561.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LT"
     gene            complement(599..>748)
                     /locus_tag="N7A07_005122"
     CDS             complement(599..>748)
                     /locus_tag="N7A07_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2479562.1"
                     /translation="DENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWEN
                     IGGMK"
BASE COUNT          223 a          204 c          132 g          189 t
ORIGIN      
        1 gtcagtgggt ggggtaagcc ttcaccaacc aaacgagaaa atgcagcggc atcaaataaa
       61 gtacatacat ctgaaccatc tgaattcttc acaaaaatac ctttttcagg ctgctccagt
      121 gtaattggac actgaatagc ctcaagcggg cactgaagtc tctctggaca aacagaaaac
      181 ttacattgag atattttgtt ctgaatactc tcttgcgatt gtgcttcacc tgagtccgaa
      241 agcatactta gcattctctc accaagggct cgaggaccac tattaaatcc caaaagcaat
      301 aactcagaaa gcaatccgcc attaccgcca ctggatgaaa agcgcccatt agtagaatca
      361 tacacaatac tgacagtttc accaccgact gtaatatctc cgcgcccatt ggctacaatt
      421 cgggcacgaa caacctccac cccaagatta aatgaatgtg atctaatatc tgaggttaat
      481 ggcataacaa agctccctat tttaatttga actccagact taaatagctg taacaaacat
      541 cccccatacg tgacaacaaa aaaccggagc cggactccgg tttttgtgaa gctgtcggct
      601 atttcatccc gccaatattt tcccacgtcc cgtcagcacg caggatttgc agcggtctta
      661 ccacacactg tatctgcttt ttatccgcat ccagtatcac cacctgcgtg attaccctgt
      721 cctgctccgg gataatacca ttctcatc
//
LOCUS       DBFIFS010000515          744 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.525, whole genome shotgun sequence.
ACCESSION   DBFIFS010000515 DBFIFS010000000
VERSION     DBFIFS010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.525"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            147..716
                     /locus_tag="N7A07_005123"
     CDS             147..716
                     /locus_tag="N7A07_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU2479563.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          211 a          156 c          192 g          185 t
ORIGIN      
        1 gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt tcaggatgac
       61 ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc tgtgagacaa
      121 ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt cacggcaact
      181 ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct gtcgttgcgg
      241 ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt gcgtcaacta
      301 taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg tccatgccac
      361 ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa cccgaaatcc
      421 gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag tactggacga
      481 agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac caggcttgtg
      541 ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga
      601 aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa gcgaatatgc
      661 tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg gattgatggg
      721 gtggctaatg atatcagata aact
//
LOCUS       DBFIFS010000516          740 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.526, whole genome shotgun sequence.
ACCESSION   DBFIFS010000516 DBFIFS010000000
VERSION     DBFIFS010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.526"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>740
                     /locus_tag="N7A07_005124"
     CDS             <1..>740
                     /locus_tag="N7A07_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGU2479564.1"
                     /translation="THNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDREFIA
                     SVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNEPLA
                     NIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYNDNY
                     LKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKMRDA
                     APKLIDFAKSFDPNSKEKIKIYTGTSKL"
BASE COUNT          248 a          114 c          152 g          226 t
ORIGIN      
        1 actcataatt tatggactgc acgtgaaaaa aatgtgacgt taattaagca aataaacgat
       61 ctcaaaatgt gtgtcaatga gtatattgga gattgtactg atgaggaatt aaacgatcgt
      121 gaatttattg catcagttgt tgacagggct atttttcatt tcgcgattaa tagtatatgt
      181 aatcccgagg ataataaaga tgcgacactc atagaacgat gtacttttga tgtagaaacc
      241 aagaatggtc ttccctccac ggttcagcta ttttatgagg aatctaaaga taatgaacct
      301 ttagcgaata tacatttgca agcaataggt tctggtttct taacgtttgt taatgcctgc
      361 caggaacatg atgacaacag cttaaaatta tttgcttcgc tgttaatttc cctttcatat
      421 tctagtgtct actcagattt tgcaggaaga gtgaatatta atgaatataa tgataactac
      481 ctgaaagctc agtttgaaga attatctcaa cgtgatatga agaagtacct gggagagatg
      541 aagcgtctgg cagatggggg agaaatgaat tttgatggct atctggataa gatgtcacat
      601 ctggtgaatg aaggaacgct cgcccctgat attttaagca aaatgcgaga tgctgcaccg
      661 aaattaattg actttgctaa gtcgtttgac ccaaactcaa aggaaaagat taaaatatat
      721 acaggcactt ctaaattaat
//
LOCUS       DBFIFS010000517          734 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.527, whole genome shotgun sequence.
ACCESSION   DBFIFS010000517 DBFIFS010000000
VERSION     DBFIFS010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.527"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            75..>734
                     /gene="tccP"
                     /locus_tag="N7A07_005125"
                     /gene_synonym="espF(U)"
     CDS             75..>734
                     /gene="tccP"
                     /locus_tag="N7A07_005125"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HGU2479565.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWP
                     APPLPVQNEQSRPLPDVAQRLVQHLAEHGIQPARSMAEHIPPAPNWPAPPPPVQNEQS
                     RP"
BASE COUNT          215 a          212 c          133 g          174 t
ORIGIN      
        1 gcatttattt gccactataa aaaggctgat tacatatcta tcggagaact aaaattactt
       61 ataaggtaat ctgcatgatt aacaatgttt cttcactttt tccaaccgtc aaccgcaata
      121 ttacagctgt atataaaaaa agcagcttct ctgtatcacc acagaaaatc acattaaatc
      181 ctgtaaaaat cagctcacct ttttcaccaa gcagtagctc catcagcgca acaactctct
      241 ttcgagcccc aaacgcccat tcggcatcat ttcatcgaca gtctactgct gaaagttcgt
      301 tacatcaaca acttcctaat gtgaggcagc gcctgataca acatcttgca gagcatggca
      361 ttaaacctgc ccggagtatg gctgaacata ttcctccggc acctaactgg cctgcgccac
      421 caccgccagt acaaaatgaa caatcaagac ctctgcctga tgtggctcag cgtctggtgc
      481 agcatcttgc agagcatggc attcaaccag cccggaatat ggctgaacat attcctccgg
      541 cacctaactg gcctgcgcca ccactgccag tacaaaatga acaatcaaga cctctgcctg
      601 atgtggctca gcgtctggtg cagcatcttg cagagcatgg cattcaacca gcccggagta
      661 tggctgaaca tattcctccg gcacctaact ggcctgcgcc accaccgcca gtacaaaatg
      721 aacaatcaag acct
//
LOCUS       DBFIFS010000518          731 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.528, whole genome shotgun sequence.
ACCESSION   DBFIFS010000518 DBFIFS010000000
VERSION     DBFIFS010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.528"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..615
                     /locus_tag="N7A07_005126"
     CDS             <1..615
                     /locus_tag="N7A07_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2479566.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
BASE COUNT          220 a          136 c          195 g          180 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg g
//
LOCUS       DBFIFS010000519          731 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.529, whole genome shotgun sequence.
ACCESSION   DBFIFS010000519 DBFIFS010000000
VERSION     DBFIFS010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.529"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            505..693
                     /locus_tag="N7A07_005127"
                     /pseudo
     CDS             505..693
                     /locus_tag="N7A07_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          216 a          141 c          155 g          219 t
ORIGIN      
        1 tactgatgta caaaacacaa caatagcttc atgatatata aatacgtgat gacgagtggt
       61 actccgcatg gttatttaac ttctaagcac cactaatgat cctgagcaac ttcgtatttg
      121 ggccattacc atggagtaga aagctgtgtc tgagaatgca gaatttgaga aaagaattcg
      181 ctttcttgga gaaattctga aaatggcacg ctaacaacgt tttgttaaaa aatgtgaatt
      241 acttgccggg gctgcatttt ctattcgagg agaatgttga cgtagatatt acagaaatct
      301 cagcgcatca ggataaactg ctaccttagg tcaaccacca aaagaaaata caatacgtcc
      361 agtttgtatg cctttaataa cacatcttct aaatgttgaa gaattatccc gcctgctgaa
      421 gaacgttgct ctgagtgtaa taaacctttt tattcttgat gatgtcagcg agaagatgga
      481 atatatcccc gactgggtcg tggttacccg gttaaacatc tggtggtgct aataaatctg
      541 gcaagtcaca ttctgttgaa tgctgtgaca gtgccttacc gagagagcga aattgtggtg
      601 ttctagtgca tagtcgccac cattccggat aattctatta cgttgtttta caagttcttt
      661 tacagcgcag atctgtcgct gacgttgaat caatagcctg gatttcagaa cacaatgccc
      721 gtttgctggt t
//
LOCUS       DBFIFS010000520          725 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.530, whole genome shotgun sequence.
ACCESSION   DBFIFS010000520 DBFIFS010000000
VERSION     DBFIFS010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.530"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>725
                     /locus_tag="N7A07_005128"
     CDS             <1..>725
                     /locus_tag="N7A07_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479567.1"
                     /translation="INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTY
                     RELNKAAQRSITAPDRIGEGKIWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSP
                     KDKYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQK
                     SQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPE
                     EEKGYWFHAYQPEDLCTIHNPMG"
BASE COUNT          249 a          157 c          133 g          186 t
ORIGIN      
        1 tattaatgct tgtggagttt atcctcagga tttaaaagac agagaaatta tatcaacttt
       61 tcacgcagaa aaagtcatta atgatctgtt aaaaaacgat tataaaattt ccctttctcc
      121 agatacaact taccgagagc tgaataaagc tgcacagcgt agcattacag cgccagacag
      181 gataggagaa gggaaaatat gggtttatca acgagataca atggttgaaa gaggtgataa
      241 cagcggtgtt catcagtatg gtccagctga acatttcact cacattatat ctgacaaacc
      301 gtccccaaaa gataagtatg ttgcatatgc tattaacatt cctgactatg agctagcagc
      361 cgatgtatat aatattaacg tgacgtcacc ttccggacag caagaaacat ttaaaatatt
      421 aatcaatcca gaacatctac ggcaaacact tgagcgtaaa tcccttactg ctgttcagaa
      481 atcacaatgt gaaatcatca cccccaaaaa acctggcgaa gcgattcttc atgcttttaa
      541 tgccacctac cagcaaatca gggaaaatat gtctgagttt gcacgttgcc attatgggta
      601 tatacaaatc cctccagtga caactttccg cgccgacgga ccagaaactc ccgaagaaga
      661 aaaaggttac tggtttcacg cttatcaacc cgaagatctt tgtaccatcc ataatccaat
      721 gggag
//
LOCUS       DBFIFS010000521          714 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.531, whole genome shotgun sequence.
ACCESSION   DBFIFS010000521 DBFIFS010000000
VERSION     DBFIFS010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.531"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            43..96
                     /locus_tag="N7A07_005129"
     CDS             43..96
                     /locus_tag="N7A07_005129"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479568.1"
                     /translation="MLIAKKDGQVLNVIHTK"
BASE COUNT          236 a          128 c          154 g          196 t
ORIGIN      
        1 atcgagggaa aacaaccgaa gaattcagga ggaaatttta caatgctaat agcgaaaaaa
       61 gatgggcaag tgttgaatgt aatacacaca aaataatcca ctgtacagtc gattcaaata
      121 ttttaccaag gtgtgtttcg taacaatttt gtgtacaata tgactctaca tatcgacgaa
      181 aaaaccactt gatttgacag gaatacattc aaaaaatttc ttttttcatt tcaggagcga
      241 ttttcatctg ttacttgaag tatctgtgag attaagtgac tccccctaac gtcgtactaa
      301 atagtaatta ctggccatac cgggagcagt attcggcgct ccccacatgc agcagaataa
      361 ccgcatcagc gaagatgtat ttttactatc acaatgtgta cggacggttt atggaaaaga
      421 atgaacatta gatacgcgat gcggcggcag gttacacggt tatcactacg atagccaata
      481 ctggctggtg cattaccgcc gtgaacaata ggacgccgta ctgctggaaa tttcttacct
      541 ttataactcc ccgcgccgcc gtatcgggaa acgggaagca atcaagatga ggctgatata
      601 atgtgcggaa tgcctgagta tttttagaat aggggggtaa tatgtttgat agaatatatc
      661 ttggtgatcg agcaataaaa aaattgaatt tgatttatgg agtaaacaaa ttag
//
LOCUS       DBFIFS010000522          709 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.532, whole genome shotgun sequence.
ACCESSION   DBFIFS010000522 DBFIFS010000000
VERSION     DBFIFS010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.532"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..167
                     /locus_tag="N7A07_005130"
     CDS             <1..167
                     /locus_tag="N7A07_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2479569.1"
                     /translation="KSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIM
                     DADQQKQHCK"
     gene            317..>709
                     /locus_tag="N7A07_005131"
     CDS             317..>709
                     /locus_tag="N7A07_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGU2479570.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          226 a          166 c          149 g          168 t
ORIGIN      
        1 ataagtctgc gttcatgaat ctcacaagag aacatctacc ccaccctctc agcagggaaa
       61 agatagtaaa agaaatgatt attgaaagga atatgtgtta ttttgacacc ataagtcagc
      121 atttcataat tatggatgca gaccaacaga aacagcattg taaataaaat gtaataatta
      181 catactatta gtgattctca tgcatcgtaa gcggctcgcc agaaccgtat tgatatttac
      241 tgaggagcct gtacatagat ttgtgtaatt gcctgatttt gatatgttca atccaacatc
      301 aaaagcaggt taatttatgg acgaaaaaca gttgcaggct ctggctaacg aactggccaa
      361 aaatctcaaa acccctgaag atctcagcca gttcgatcgc ctgctgaaga aaatcagcgt
      421 tgaggcagct ctcaacgctg aaatgtccca ccatctgggc tacgataaaa accagcctaa
      481 accgggtgcc aactcccgca atggctattc cacaaagacc gttatcaccg gtgatggcca
      541 cctggaacta cgcactccgc gcgatcgtga tggctctttt gaaccgcaac ttgtgaagaa
      601 aaaccagacc cggatcaccg ggatggataa ccagatcctg tcgttgtacg ccaaaggcct
      661 gacaacccgc gaaatagcgg ctgcgtttaa agagctgtat gacgctgat
//
LOCUS       DBFIFS010000523          703 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.533, whole genome shotgun sequence.
ACCESSION   DBFIFS010000523 DBFIFS010000000
VERSION     DBFIFS010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.533"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            23..574
                     /gene="ybcL"
                     /locus_tag="N7A07_005132"
     CDS             23..574
                     /gene="ybcL"
                     /locus_tag="N7A07_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGU2479571.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVASNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            584..>703
                     /locus_tag="N7A07_005133"
     CDS             584..>703
                     /locus_tag="N7A07_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2479572.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKK"
BASE COUNT          211 a          128 c          168 g          196 t
ORIGIN      
        1 aggtcataac aaagaggttt ttatgaaaac acttatcgtt tcaactgtat tggcattcat
       61 aacattttct gcgcaggctg cagcatttca ggtcgctagt aatgaaataa aaacaggaga
      121 gcaacttaca acgtctcatg tcttttctgg atttgggtgt gaaggtggta atacatcgcc
      181 ctcattaacc tggtctggtg ttcctgaagg taccaaaagc tttgccgtaa ctgtatatga
      241 tccagatgca cctacaggca gtggttggtg gcattggact gttgttaata ttccagcaac
      301 agtaacatat ttgcccgttg atgcagggag acgtgatgga acaaaactgc cgactggtgc
      361 tgttcaaggc cgaaatgatt ttggctatgc tgggtttggt ggcgcatgtc ctcctaaagg
      421 agataaacca catcattacc agtttaaagt atgggctcta aaaactgaaa agattcctgt
      481 agattctaac tccagcggag cgttagttgg ttatatgctt aatgctaata aaatcgcaac
      541 cgctgagata acaccagttt atgagataaa gtagggtgag agtatgctgg caagaggtaa
      601 gactaactta aagatcgaag aaatacggat gcataaacat catgagattc atagggttaa
      661 gcctcttatg ccagctttgt gtcgtatccg tcagggaaag aaa
//
LOCUS       DBFIFS010000524          691 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.534, whole genome shotgun sequence.
ACCESSION   DBFIFS010000524 DBFIFS010000000
VERSION     DBFIFS010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.534"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(1..691)
                     /locus_tag="N7A07_005134"
                     /pseudo
     CDS             complement(1..691)
                     /locus_tag="N7A07_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
BASE COUNT          199 a          154 c          129 g          209 t
ORIGIN      
        1 tgtttgtcca tcttttcata aacattactg acagctgagt tattattgga caaaattgcc
       61 actgttttat tctgaattaa tatattagca acaatattga gtatagtctg tgttttacct
      121 gttccaggtg gtccttcaat aactgaaatc tgtgaggaaa atgcccgttc gacagccagc
      181 aactgacttt cattaagacc aaacggaaaa atcaaacccc atggcgaatc acgtttttac
      241 tctgtcctga acagtatgca tgcaaggcgg tatccggatg aggaactatt ttctttatct
      301 gcctaaggat attctcatcg atatttctgt tattaccacg ggcataaaaa atacgttcat
      361 tagccaccct acacaagtaa ttaaacacgg catgctctgt aaatgctgaa cactgttgaa
      421 agtcaaggcc ggtcgttttc atgatgtagg gtcgttcacc ctcgcggtaa taaacagcag
      481 catatttatc accatatata ataaccagtt cagcccggga tgttacagcg ttccctttcc
      541 tttcgaggaa aacatttcgc aaatttacag ttggcgttat ctgacataca gaaagcggac
      601 gagtatattt tctcccgctg cgaaaatgac aggtcagcat aacctctctg tcgtcatttc
      661 cagcccacag cgtccagtcg gtaattttat g
//
LOCUS       DBFIFS010000525          686 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.535, whole genome shotgun sequence.
ACCESSION   DBFIFS010000525 DBFIFS010000000
VERSION     DBFIFS010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.535"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..79)
                     /locus_tag="N7A07_005135"
     CDS             complement(<1..79)
                     /locus_tag="N7A07_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2479573.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="N7A07_005136"
     CDS             complement(91..489)
                     /locus_tag="N7A07_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU2479574.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..>686)
                     /locus_tag="N7A07_005137"
     CDS             complement(531..>686)
                     /locus_tag="N7A07_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2479575.1"
                     /translation="ADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
BASE COUNT          140 a          218 c          178 g          150 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgc
//
LOCUS       DBFIFS010000526          683 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.536, whole genome shotgun sequence.
ACCESSION   DBFIFS010000526 DBFIFS010000000
VERSION     DBFIFS010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.536"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            50..>683
                     /gene="escD"
                     /locus_tag="N7A07_005138"
     CDS             50..>683
                     /gene="escD"
                     /locus_tag="N7A07_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU2479576.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNN"
BASE COUNT          203 a          140 c          130 g          210 t
ORIGIN      
        1 accatatttt ttctcattgt tcaaccacca ggatgaataa aatttaaaaa tgttatcctc
       61 atataaaata aaacttctca atggtgcgat gcgtaatcgg gaattacaac ttccgatggg
      121 aaacctaacg ctaggtacgg aagataacga tattgtttac tttccccttg aacaaggcct
      181 caatcagttt ttacttgata tacgtgagga aggtgttttc cttttatctc cggttgaatt
      241 ttggattgat gggcaaccca cgccctatga agcagataaa ccattacctg tcggaaaggt
      301 tattgatata gcgggatgtt gcttcatcat tggtgatatt gaccattcct tgccattgtc
      361 tgatgtgcca gaaaggttct ctgccaaaag tcgacgcaaa aaacgcctta tcctggcgag
      421 cgtgattggt gctacttttg cgttgtctgg tgccatcggc agttacgttt tgctttcccc
      481 aaaagctgaa cccccaacgt ttaccagagc tgatgtatat cagcagctaa aagaaaacaa
      541 acttcatgca atcactctgg tatggcatgg gaaaaatatc gcgctttatg ggcgttgcga
      601 atcaaccacg gatttaactc cattttttaa ttacctaaaa gaaaaaaata ttttttatta
      661 caataatatc atttgcaata acc
//
LOCUS       DBFIFS010000527          672 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.537, whole genome shotgun sequence.
ACCESSION   DBFIFS010000527 DBFIFS010000000
VERSION     DBFIFS010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.537"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..418
                     /locus_tag="N7A07_005139"
     CDS             <1..418
                     /locus_tag="N7A07_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2479577.1"
                     /translation="VSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSS
                     QWLKIAANNGHATAQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVNCYK
                     NQGNKKEALQWAYKLKASKQPLDADRLIEELTKEQK"
     gene            complement(421..>672)
                     /locus_tag="N7A07_005140"
     CDS             complement(421..>672)
                     /locus_tag="N7A07_005140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479578.1"
                     /translation="KIYAKSCAGHILNDTKSYIFIFSLATFDASAKYIVMITGDEDAS
                     FGFNLRFDELVAVVVNVILCVGVVATGKVFLLSIFLLPV"
BASE COUNT          253 a          111 c          145 g          163 t
ORIGIN      
        1 agtaagcgtc ttttatttgc agccagaaga agtaaatact ttatcacacc aggcaaaacg
       61 aggcgatggc gaggcgggtt ttaggttgtt cttgtactac aagttgtcca attatgatga
      121 agaaaaaagt agtcagtggt taaaaatagc ggctaataat ggtcatgcga cagctcagta
      181 tcggttatat gtagagttac aagaacaagg tgaaaccaag gaagctaatg aatggttaca
      241 aaaaattaaa ttggctgcca gtaataatga cagttcagca atatggttct tggtgaattg
      301 ttataaaaac cagggaaata aaaaagaagc attgcaatgg gcatataaac tcaaagcgag
      361 taaacagccg cttgatgcag atcgactaat tgaagaatta acgaaagaac aaaagtagct
      421 ttaaacaggt aacagaaaaa tggagagtag aaaaacttta ccagtcgcta caacacctac
      481 acacaaaatt acgtttacga cgactgcaac aagctcatca aagcgcaagt tgaaaccaaa
      541 actggcatct tcgtcgccgg ttatcattac gatgtacttg gcagatgcat caaaagtcgc
      601 aagagaaaag atgaaaatat agctttttgt atcattaagt atgtggccag cacatgactt
      661 ggcatagatc tt
//
LOCUS       DBFIFS010000528          662 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.538, whole genome shotgun sequence.
ACCESSION   DBFIFS010000528 DBFIFS010000000
VERSION     DBFIFS010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.538"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..63)
                     /locus_tag="N7A07_005141"
     CDS             complement(<1..63)
                     /locus_tag="N7A07_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2479579.1"
                     /translation="MKACLLLFFYFSFICQLHGAD"
     gene            complement(75..>662)
                     /locus_tag="N7A07_005142"
     CDS             complement(75..>662)
                     /locus_tag="N7A07_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479580.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          194 a          146 c          112 g          210 t
ORIGIN      
        1 atcagcacca tgcaattgac aaataaaaga gaaataaaaa aatagtaaca agcatgcttt
       61 catacatcta caacctatct tatatcactg ggtttttctg gcgtaccttg catattagaa
      121 atggggtgcc cagatggccc atatgttatc caattaccaa ggtcttttgt tgttatagcc
      181 attagatcct tatttgaatt aaggttcgtt ggtgctgtta gcactgtatc catttttaac
      241 ttaccattag ttcctcgccc ccaccacgtc catagcgtgt caggtcctat tacatttgta
      301 tgtaactctt tagctaattg gctagcaaat gaattttgtc ctttacctgt attacaagaa
      361 aataaccaaa ctgtcatccc atctttataa ttcccatcaa atttaattag atatgctaga
      421 tctttagctg tcatgatact gcgcgttgcg gattcaatag atgtgggggt accatgcccc
      481 ccgattgtga aaacgcctgg gatattaatc tcatcagcta cagaatggat aagatcactt
      541 tcggggtata aatttatatc aaccaaacct tgcggatcta catttatgac cggattcaac
      601 ggatactgat aaaaattcca tccccccttc agtccaatcg gatcctgagt gatatatcgc
      661 cc
//
LOCUS       DBFIFS010000529          662 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.539, whole genome shotgun sequence.
ACCESSION   DBFIFS010000529 DBFIFS010000000
VERSION     DBFIFS010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.539"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(27..601)
                     /locus_tag="N7A07_005143"
                     /pseudo
     CDS             complement(27..601)
                     /locus_tag="N7A07_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
BASE COUNT          210 a          151 c          108 g          193 t
ORIGIN      
        1 ccaatactct ctcagagaaa aatattttaa ctatctttta taatgaagtt tcccttgata
       61 ggatcataaa cacatttatc aggacttata atcattgatg ccgttattgg ttctcgggtc
      121 agtggatgat atgagccttc actagctaaa cgagaaaatg catcaaaatc aaataagcag
      181 catactgccg aactatctga atttttgaca aacactcctt cttcgggtct ctctagtgta
      241 attggacact gaatagactc tggcgggcac tggaaatttc ctgaactaac aggaaactta
      301 cattgagata ttttgtcctg aatactctct tgcgattgtg cttcacctga gtccgaaagc
      361 atactgagca tcctatcgcg aagagcttca gggccgttat taaacccata aaggaataac
      421 tcagtaagca atcccccact actgccgctg gatagaaaac gcccatcggt ttcattaaat
      481 acaatgctga cagtctcatt acctaatgta aactcccctc gaccgctaga tgctacttga
      541 gctcgaagga cattcaatcc attaaaaatg agcttgatct aatctccgaa tttaacggca
      601 ttataaaact ccctatttta atttaaactc cagacttaaa tagctttaac aaacatctgc
      661 ct
//
LOCUS       DBFIFS010000530          657 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.540, whole genome shotgun sequence.
ACCESSION   DBFIFS010000530 DBFIFS010000000
VERSION     DBFIFS010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.540"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..103)
                     /locus_tag="N7A07_005144"
     CDS             complement(<1..103)
                     /locus_tag="N7A07_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479581.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIF"
     gene            complement(100..>657)
                     /locus_tag="N7A07_005145"
     CDS             complement(100..>657)
                     /locus_tag="N7A07_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2479582.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          173 a          142 c          126 g          216 t
ORIGIN      
        1 cgaaaatagt aaaacaatca tgatctttag cttgtatttc gcaatcagct ataacaccaa
       61 aactaacaaa tatcatcagc aaaaaaaaca aaactctttt cattttctta ttcctctcga
      121 tgttctctgt ttcattttag ctaaaatttc acccaaatca tctatccctg ctgttgctgg
      181 cccatcgggt ttattaatgt cagggaataa atagggttct gcacaagtat atcctggtgg
      241 taattgagtt gttggccaat ttgtctgtgg caggtaatca cttgctgtct ttgtatccat
      301 ccctggatac tggggatcat cctgtggcgt acaagtcaga tttggtttta cccacttcct
      361 gcaatacagt gtgtagtcgt aagggcttaa tgctctgaag aatgttttga aaacattagg
      421 cgcactactg gcctggtcaa taggatatgg cagtcttaat tgtttcgcta tgtctggaaa
      481 tttacttgga tcgggcgtag gcggtttgtt gggtctcgct ccatatccgc cattatttaa
      541 gtgtccatat tttgatgcca gtcccattga atctatatac tgaaccggat tcaacggata
      601 ctgataaaaa ttccatcccc ccttcagtcc aatcggatcc tgagtgatat acctccc
//
LOCUS       DBFIFS010000531          652 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.541, whole genome shotgun sequence.
ACCESSION   DBFIFS010000531 DBFIFS010000000
VERSION     DBFIFS010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.541"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(202..>652)
                     /gene="escD"
                     /locus_tag="N7A07_005146"
     CDS             complement(202..>652)
                     /gene="escD"
                     /locus_tag="N7A07_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU2479583.1"
                     /translation="LLLNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKND
                     VIIVAGEVSQQNESKILAIINAMNKNSNAKILFQNIQPYISADIFPGKILRISGTMKN
                     PTIALDNGTSLGIGSILKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT          188 a          133 c          116 g          215 t
ORIGIN      
        1 agaaataacc tcttataatc aaatctacta ctggtctttt tatctgctta ataggtctct
       61 ttcaaagaga cacattcacg ttttctcatg taggttgatc caaccacgct gataccaaag
      121 ctgaatcaca tcaagcaaca actatgctca caacatccac acaataaaaa accctccgaa
      181 gagggggaag aggataagaa attaatacga cagtggaata tgtatatatt cgtctgggcg
      241 ggagatgttg ataccatctt taggatcaat cgcgtctatg acatagcctc ctttcagaat
      301 gctaccaatc cccaggctgg tgccattatc aagagcaatt gttggattct tcatcgtacc
      361 gctgattctg agtatttttc cggggaaaat atctgctgaa atgtacggct gaatgttctg
      421 aaagagtatc ttggcgttac tatttttgtt cattgcattt ataatggcaa ggatcttact
      481 ctcattctgt tgagaaactt cccccgcaac aatgatgaca tcattctttt tgaaaatatt
      541 gacatagttt attagtttat tctttttaaa ctctgatgcc agttcattga ttttattgtt
      601 actgttgttg tgtatttccc atcctgcaac tcctggcgta ttcagtaaca ga
//
LOCUS       DBFIFS010000532          634 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.542, whole genome shotgun sequence.
ACCESSION   DBFIFS010000532 DBFIFS010000000
VERSION     DBFIFS010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.542"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>634
                     /locus_tag="N7A07_005147"
     CDS             <1..>634
                     /locus_tag="N7A07_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HGU2479584.1"
                     /translation="FHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSR
                     KPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVL
                     QEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPER
                     KLHLYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLP"
BASE COUNT          143 a          167 c          241 g           83 t
ORIGIN      
        1 tgttccacac gcggatacag cacggcgaac cacaggtgga gagccggtac ctctatgacc
       61 cgctgggccg ccggacggga aaacgggtgt ggcggcggga gcgtgacctg acggggtgga
      121 tgtcgctgtc gcgtaaaccg gaggagacct ggtacgggtg ggacggtgac aggctgacca
      181 ctgtacagac ccaacagaca agaatccaga cggtatacca gccgggaagc ttcacgccgc
      241 tcctgagaat cgaaacagag aatggtgaac aggcgaaggc gcggcaccgt agcctggcgg
      301 aggtgttgca ggaggacacg ggtgtgacgc taccggcgga gctggcggtg atgctgggga
      361 ggctggagcg ggaactgcgg cagggcagcg tgagtgaaga aagccagcag tggcttgcgc
      421 agtgcgggct gacggcggaa cagatggccg cgcagctgga ggcggaatat atcccggagc
      481 ggaaacttca tctttaccac tgcgaccacc ggggactgcc gcaggcgctc atcagcccgg
      541 aaggggaaac ggcgtggcag ggggagtatg acgagtgggg aaacctgctg ggcgaaacca
      601 gcgcgcagca cctgcaacag ccgtaccgtc tgcc
//
LOCUS       DBFIFS010000533          626 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.543, whole genome shotgun sequence.
ACCESSION   DBFIFS010000533 DBFIFS010000000
VERSION     DBFIFS010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.543"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            255..470
                     /locus_tag="N7A07_005148"
     CDS             255..470
                     /locus_tag="N7A07_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2479585.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            475..>626
                     /locus_tag="N7A07_005149"
     CDS             475..>626
                     /locus_tag="N7A07_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HGU2479586.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVAPEIGGYPSLLLPDVAISLDR
                     TEGATL"
BASE COUNT          150 a          122 c          186 g          168 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgggtgt taaagggata aataattacc cggataagat tactgttact
      541 gtggcaccgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc gattagtctt
      601 gaccgtactg aaggtgccac gctgga
//
LOCUS       DBFIFS010000534          623 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.544, whole genome shotgun sequence.
ACCESSION   DBFIFS010000534 DBFIFS010000000
VERSION     DBFIFS010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.544"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..>623)
                     /locus_tag="N7A07_005150"
     CDS             complement(<1..>623)
                     /locus_tag="N7A07_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU2479587.1"
                     /translation="TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLT
                     AVVSPDGLESRRAYDEPGRLVSETSRSGETVRYRYDDAYSELPATTTDATGSTRQMTW
                     SRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHE
                     TRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           88 a          212 c          177 g          146 t
ORIGIN      
        1 aggtcgtatt ccatactgcg cgtcagcccg ccctgcgtgg tgctgaccgc ctttccccac
       61 gcatcgtact gtgtctcgct ccggttgccg tccggggtga taacggcagt caggtcgcct
      121 gcggcgttgt actcataccg cgtttcatgg ccctgtgcgt ctttcaccga gattaaccgg
      181 ccacggttgt catagcggcg gtaaaggctg atgccttcct cgcggtggac cgccgtcatc
      241 tggccgaagc ggtcgtattc atagcgggtc tggtagcccg agcagtcggt gaacgccagc
      301 aactgaccgt agcggctcca ggtcatctgc cgggtgctgc ccgtcgcatc tgttgtcgtc
      361 gccggtaact cactgtacgc atcatcgtag cggtagcgta ctgtctcccc gctgcgcgat
      421 gtctccgata ccagcctgcc cggttcatca tatgcccggc ggctctccag cccgtccggg
      481 gacaccaccg ccgtcagctg gttcccgtcg ttatagtaaa atttcgtctc ccgcccgtcc
      541 ggtgtggtga tgtccgtgat atcgccggat accacattca gaccatactc tgtccgccgt
      601 cccgccgcgt ccgtctgcgc cgt
//
LOCUS       DBFIFS010000535          623 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.545, whole genome shotgun sequence.
ACCESSION   DBFIFS010000535 DBFIFS010000000
VERSION     DBFIFS010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.545"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            52..>623
                     /gene="fimB"
                     /locus_tag="N7A07_005151"
     CDS             52..>623
                     /gene="fimB"
                     /locus_tag="N7A07_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU2479588.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRA"
BASE COUNT          173 a          123 c          143 g          184 t
ORIGIN      
        1 attgctaacc tagcacagct agtgcgcgtc tgtaattata agggaaaaac gatgaagaat
       61 aaggctgata acaaaaaaag gaacttcctg acccatagtg aaatcgaatc actccttaaa
      121 gcagcaaata ccgggcctca tgcagcacgt aattattgtc tgactttgct ttgttttatt
      181 catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga tcttaaggca
      241 aagtgtatat atatccatcg attaaaaaaa ggcttttcaa caacgcaccc gctattgaac
      301 aaagaagttc aggctttaaa aaactggttg agtatccgta cttcttatcc gcatgctgag
      361 agcgagtggg tatttttatc gcgtaagggg aatccgcttt ctcggcaaca attttaccat
      421 attatctcga cttccggtgg taatgccggg ttgtcactgg agattcatcc gcacatgtta
      481 cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact tatccaggat
      541 tatcttgggc atcgcaatat tcgtcatact gtctggtata ctgccagcaa tgcagggcgt
      601 ttttacggca tctgggatag agc
//
LOCUS       DBFIFS010000536          623 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.546, whole genome shotgun sequence.
ACCESSION   DBFIFS010000536 DBFIFS010000000
VERSION     DBFIFS010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.546"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(387..>623)
                     /locus_tag="N7A07_005152"
     CDS             complement(387..>623)
                     /locus_tag="N7A07_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2479589.1"
                     /translation="KYEFNHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTF
                     YRQISLLAKEKPVEGSPLFLLQMCTEQLVVSGKI"
BASE COUNT          184 a          127 c          123 g          189 t
ORIGIN      
        1 ttttattacg aagaccttta cgcatgttag aaagtatcat tattgttaat aaatacggta
       61 ataagtaaca aacagatatt gataacttcc ctgacaagtt attaggtatc ccacttgaag
      121 attgagaatg gctccagagt aaactgtgta ctgttcgccg gagtgtcatt actctcaaca
      181 atcggttttg acagtgagtc gggcgcttca taccggtttc tacctggagt cattgtcact
      241 tctcccaact ggataaatat atcgccaatt cacaaagtgg gccaaaatca ggtgaaaacg
      301 aattattacg cctggtaaag gctatagtac aactaagtgt ggcgaatcag gcagtcttgc
      361 atttgtcatc aatctcatgt cttacatcaa atttttccac tcaccactag ttgttccgta
      421 cacatttgta gtaaaaacag tggactgcct tctaccggct tttctttagc cagcaacgaa
      481 atctgtcgat agaaggttgg ttcgagagga acggaacaca attcatctgc aatggttttc
      541 agcgttaact ctggtaataa ggcgatgcct aatccctgtc gaataaaact gatcgctgta
      601 tccgggtgat taaattcata ttt
//
LOCUS       DBFIFS010000537          622 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.547, whole genome shotgun sequence.
ACCESSION   DBFIFS010000537 DBFIFS010000000
VERSION     DBFIFS010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.547"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..507
                     /gene="ydeO"
                     /locus_tag="N7A07_005153"
     CDS             <1..507
                     /gene="ydeO"
                     /locus_tag="N7A07_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU2479590.1"
                     /translation="TGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFITLLT
                     NGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARM
                     QHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTG
                     NTMNALAI"
BASE COUNT          197 a          119 c          125 g          181 t
ORIGIN      
        1 actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat
       61 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccacatgcaa aggtttcatt
      121 acactattaa ctaacggtgt gctatccgtt tcggggaaag tgagaaatat tgtcaacatg
      181 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc
      241 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct tttagatgcc
      301 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      361 caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      421 agtccaaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaatacgggc
      481 aacacgatga atgctttagc tatttgatta tttgctaacg agtagtcaac cacgcaccct
      541 acgtaaaaat taaaaatggg gcagtcattc cctgccccgc gttgttttta ggcgatatat
      601 ttattgaaat aaataagtga ca
//
LOCUS       DBFIFS010000538          617 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.548, whole genome shotgun sequence.
ACCESSION   DBFIFS010000538 DBFIFS010000000
VERSION     DBFIFS010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.548"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..>617
                     /locus_tag="N7A07_005154"
     CDS             <1..>617
                     /locus_tag="N7A07_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU2479591.1"
                     /translation="LDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDR
                     LTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPET
                     GELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRL
                     HRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNG"
BASE COUNT          147 a          176 c          205 g           89 t
ORIGIN      
        1 cttgaccggc tggtacagca gcgcggcttt gacgggcgga cgcaacgtta ccactatgac
       61 ctgaccggaa aactcacgca gagtgaagat gaggggcttg tcaccctctg gcactacgac
      121 gaatcggacc gcctcactca ccgcacggtg aacggcgaac cggcagagca gtggcagtac
      181 gacgagcacg gctggctgac agaaatcagc cacctgagcg aaggccatca ggtggcggtg
      241 cattacggtt atgatgataa gggccgcctg gccggggagc gccagacggt gcataacccg
      301 gagacggggg aactgctgtg gcagcatgag acagagcacg catacaacga acagggtctg
      361 gcaaaccgcg tcacgccgga cagcctgccg cgggtggagt ggctgaccta cggcagcggt
      421 tatcttgcgg ggatgaagct gggcgggacg ccgctggtgg agttcacgcg cgacaggctg
      481 caccgcgaga cggtgcgcag cttcggcaat aacgcatacg aactgaccag cacatacact
      541 cccgcaggcc atttacagag ccagcgcctg aacagccagg tgtatgaccg tgactacgac
      601 tggaatgaca atggcga
//
LOCUS       DBFIFS010000539          615 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.549, whole genome shotgun sequence.
ACCESSION   DBFIFS010000539 DBFIFS010000000
VERSION     DBFIFS010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.549"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..>615)
                     /locus_tag="N7A07_005155"
     CDS             complement(<1..>615)
                     /locus_tag="N7A07_005155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2479592.1"
                     /translation="TPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDI
                     GVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQ
                     FDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMR
                     IYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASG"
BASE COUNT          176 a          128 c          130 g          181 t
ORIGIN      
        1 tccctgaagc ggaaaacatt actacagtac cttgaacctt ggttttaaca ttctcgaaat
       61 gctctgagag tatatgtgtt aagccttctt cggaatcttt tgtgtttgaa aagatgcctt
      121 tctgattgta aatgcgcatc agtttttgac cgaagctatt gtgcacaact ccatcgccaa
      181 gaattgtggc tccgtataga gttccatcgt cagttaaggc ctgcgccgca ttgcgtatta
      241 cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac atggaaatgg
      301 aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg ctaattttat
      361 gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct tcgttcaaat
      421 ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca gttccaacac
      481 caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg tgttcctttg
      541 taggacatcc ccatgcaaga cgatttgata ctcccaaaac ccaccagtca taaagcttta
      601 gggtaagtgg tgtgt
//
LOCUS       DBFIFS010000540          608 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.550, whole genome shotgun sequence.
ACCESSION   DBFIFS010000540 DBFIFS010000000
VERSION     DBFIFS010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.550"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..362)
                     /locus_tag="N7A07_005156"
     CDS             complement(<1..362)
                     /locus_tag="N7A07_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HGU2479593.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMDRRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTGNKILMEKINS"
     gene            complement(435..>608)
                     /locus_tag="N7A07_005157"
     CDS             complement(435..>608)
                     /locus_tag="N7A07_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2479594.1"
                     /translation="SDGSDVCTLFDAAAFSRLTGEDLPHPLTREPITASIIVKHEECI
                     YDDTRGNFVIKGN"
BASE COUNT          182 a          133 c           99 g          194 t
ORIGIN      
        1 caagaattta ttttttccat taatatttta ttacctgttt tattttctct gtgttctgcc
       61 atgacactct gaaaatagtt attaacacta cttaaaaaat cgacacctcc atttaattgc
      121 ctttccagag ccacagccct tctatccatt cgatgctcac gccccaatag cctatccagt
      181 aagcctcctc tcaccggttc aactgaaaat ccatccagta gttggacata gtgaacagaa
      241 tattggccac taccaatgga tattcttatt ccactttcaa gtctggcagc atcctgcaaa
      301 gactgtactc ctgcaggaga taattgagat atacttggga tacttaaggt ggtaacaggc
      361 ataaaaacct cattcagtaa ttcatatcag tttgcaggat gtttcattaa ataaaataaa
      421 gggtaatgtt catttcaatt accctttata acgaagtttc ctctggtatc gtcataaatg
      481 cattcttcat gttttacaat tattgatgcc gttattggtt cccgggtcag tgggtggggt
      541 aagtcttcac cagtcaaacg agaaaatgca gcggcatcaa ataaagtaca tacatctgaa
      601 ccatctga
//
LOCUS       DBFIFS010000541          605 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.551, whole genome shotgun sequence.
ACCESSION   DBFIFS010000541 DBFIFS010000000
VERSION     DBFIFS010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.551"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..392
                     /locus_tag="N7A07_005158"
     CDS             <1..392
                     /locus_tag="N7A07_005158"
                     /inference="COORDINATES: protein motif:HMM:NF019008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU2479595.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            449..605
                     /locus_tag="N7A07_005159"
                     /pseudo
     CDS             449..605
                     /locus_tag="N7A07_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    505..>605
                     /locus_tag="N7A07_005159"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          196 a           99 c          158 g          152 t
ORIGIN      
        1 ttatgcagaa aagcaataaa gttataatca gctgtggtat ggcattatat tttttgtgtg
       61 gggggattac agtggcagaa gaacttgatg aaagcatgca agttcttgca aggaatatga
      121 gtgtacttca gtcatcaatg gataaaaagg agcttcttga agctcttgac agaatggatg
      181 atgctgtaga tgagtcaatg aagcgtcttc ctgagagcat ttcaccggct gatgaagaag
      241 ggaagaatga ctatataaaa gaacttcaaa agcttgaaaa agagattatg ctggcgaaac
      301 aaaaagtctt atcgggacag ttatcagata tacccgattc ggtaaacaag atgaataaaa
      361 tcagagttga ggggcatgat aaattcagat aaatcagttg gttgattcct gcgggacgaa
      421 tacatacgtc aggaacataa ctttatggct ggaacgtgaa aatcgtgaac tgcgccgcag
      481 taacgatatc cttcgccagg cttccgctta ttttgcgaag gcggagttcg accgcctctg
      541 gaaaaaataa tgccactgct ggataagctg cgtgagcagt acggggtcgg accggtatgc
      601 agtga
//
LOCUS       DBFIFS010000542          598 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.552, whole genome shotgun sequence.
ACCESSION   DBFIFS010000542 DBFIFS010000000
VERSION     DBFIFS010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.552"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            91..411
                     /locus_tag="N7A07_005160"
     CDS             91..411
                     /locus_tag="N7A07_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU2479596.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSVEKLSFAIKE
                     HYFD"
     gene            404..>598
                     /gene="escP"
                     /locus_tag="N7A07_005161"
     CDS             404..>598
                     /gene="escP"
                     /locus_tag="N7A07_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HGU2479597.1"
                     /translation="MTRVSLKRNLIEPVSFRPSDDWPQGEESFNDIFYHIKKRSPDTL
                     FIRMASLWKRLFYSSGRRRRY"
BASE COUNT          209 a           92 c          126 g          171 t
ORIGIN      
        1 gattgaaagc ctattcaaag tggttgcctg agtatgttgg acagaatttt atctattcgt
       61 aaaagcagag cgaaccgatt gagagaatca atggctaaga ttaactctca gataaaagag
      121 gtggatggta aactcgatga ctgcgagcaa tccattaaag agtcgattgc ctctaagcag
      181 gcatattgtg catcgctggt gaatttggat aaagtgtcgt tgtataaata tcagattaaa
      241 aacaatgcat ttgatgagca aaagcagcga ttgtatgaga aaaaatcttc actttcgaag
      301 gaaaagcgtt cattgctaga ttcacaaaaa cgaacaaaag agaatctgca acatgttaat
      361 aaatcagtag aaaaattatc ttttgccata aaggaacatt actttgacta gagtttctct
      421 aaaaagaaat ttgatagagc ctgtttcttt tcggccatca gatgattggc cgcaaggaga
      481 agaaagtttt aatgatattt tttatcatat aaaaaaaaga tcgcctgata cgttgttcat
      541 acgaatggct tctttatgga agagattgtt ttactcctcg ggccgaagac gacgctac
//
LOCUS       DBFIFS010000543          590 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.553, whole genome shotgun sequence.
ACCESSION   DBFIFS010000543 DBFIFS010000000
VERSION     DBFIFS010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.553"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(70..216)
                     /locus_tag="N7A07_005162"
     CDS             complement(70..216)
                     /locus_tag="N7A07_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479598.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          177 a          122 c          112 g          179 t
ORIGIN      
        1 taattcttga tacaatctgt ctgtcaaaat agtagattag atcgaactta tattacagct
       61 atttagtttc taaaatggaa tttttaacgt tttatacttc acattacaat ctgtaccata
      121 agcctcctca gcgaggttgc ctgaataccc tgtcgtgccc atttttccat cggacgcagt
      181 tgtctgctct ctcgaactac tggcaataaa aatcaaccag acagcttgat gtccgttaca
      241 tgcaggacta atcacgattg gttaccgttt acaaaacttg cgtaacattg gaggagaacg
      301 aataatattc tctagctggt acgcgttcca tagcgaaacg ggcaaggtcc aacagcaaga
      361 gctgaaactc agggggattt atcagtcgat ttattcgcca tggatgaatc acgctgtacg
      421 gttatggtag ctcttagcga caaggcataa tcatcagtac accaatgtaa caaccgttga
      481 aaaagactca ttattttatg aaccagccat cccatttcca gagtggtaaa tatgatttgt
      541 taaagtgtaa cagtactctg cgtagccttt ttagattcat agtttaatgt
//
LOCUS       DBFIFS010000544          588 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.554, whole genome shotgun sequence.
ACCESSION   DBFIFS010000544 DBFIFS010000000
VERSION     DBFIFS010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.554"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            333..>588
                     /locus_tag="N7A07_005163"
     CDS             333..>588
                     /locus_tag="N7A07_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479599.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDY"
BASE COUNT          198 a           86 c          115 g          189 t
ORIGIN      
        1 tggcagtgtt tgatgaaaat aaaaaaataa taggttcttc ctcaggacta agttgttttc
       61 aaccacatta aaactctatg agtgctgtgg tatatctttt taatcctgcc agtatttcat
      121 agtttttctt aatcgattgt agaggcatgt actgatgacg cctctacaat ctttagggac
      181 tttctctact atgagataag aaatttcata ttatgcttta aggctgaatg atttgtgcaa
      241 aatataatta tttttccatc gaacatgagg tgtatgtcat aagattaaat gataactatg
      301 tattcctcgc ggtgaattat atgaggtaaa agatgaaaat tggaactgtg gcaggtacta
      361 acgacagtac aacgacaatc gcaacaaatg atatggtgca agagcatgtt actaacttca
      421 ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca agtattgcca
      481 ggacaatgct cggtgaggtt ctggaaaaag tagatgattg gcaaatagaa cggttccaac
      541 aatctattca agatgacaaa atttcattta ccatacaaac ggattatt
//
LOCUS       DBFIFS010000545          582 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.556, whole genome shotgun sequence.
ACCESSION   DBFIFS010000545 DBFIFS010000000
VERSION     DBFIFS010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.556"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..>582)
                     /locus_tag="N7A07_005164"
     CDS             complement(<1..>582)
                     /locus_tag="N7A07_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HGU2479600.1"
                     /translation="KTFATFYKVINKEKYLESIQVHFLNLPSYRRFPNDDEFKRELKV
                     RDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKWREELGSDWQRIHKE
                     LLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLGQCEKWDEAAIHARA
                     DRLAELAVQVWQAPSLPEEVLAVYRGQPENKTS"
BASE COUNT          154 a          140 c          119 g          169 t
ORIGIN      
        1 taactggttt tgttctcagg ctgtccccga taaacagcta aaacctcttc aggaagagaa
       61 ggcgcttgcc agacctgaac cgcaagctcc gccagacgat cggctcgggc gtgaatggca
      121 gcttcatccc atttttcgca ctgtccaaga ccgatattca aatagagcgg gctatgctta
      181 aagccgtctt caatatcgcg tttttctgcg aaaggtctgt cactgtaacg ggagttatag
      241 cgcgttaaag tgagattccc caacgtatgc aacagttctt tatgaatacg ctgccagtca
      301 cttcccagct cttcgcgcca tttagccgac aaattttcgt tctgcggcat aatatgttca
      361 atcgtaaact cttccacgcg ctctctgcgt ttatcgtttt ccagtcgtcg taaccagtag
      421 ctacgactac ggaagttata gagatcgcga acttttaatt cccgtttaaa ttcatcatcg
      481 ttggggaaac gacgatatga aggtagattc aaaaaatgta cctgaatact ttccagatat
      541 ttttctttat taatgacctt ataaaaagta gcaaacgtct tg
//
LOCUS       DBFIFS010000546          576 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.557, whole genome shotgun sequence.
ACCESSION   DBFIFS010000546 DBFIFS010000000
VERSION     DBFIFS010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.557"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            317..>576
                     /locus_tag="N7A07_005165"
     CDS             317..>576
                     /locus_tag="N7A07_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2479601.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQL"
BASE COUNT          158 a          107 c          156 g          155 t
ORIGIN      
        1 tacgatacct gcgtcataat taattatttg acgtggtttg atggcgtaga tgcacgttgt
       61 gacatgtaga tgataattat tatcattttg cgggtccttt ccggcgatcc gacaggttac
      121 ggggcggcga cctcgcgggt tttcgctatt tatgaaaatt ttccgggatc catgtccggt
      181 ttctcttcaa gttaactata tgaaaaatat aaaaacaggt cttctgtgaa ccggacatga
      241 acaaaaaaca gacatgtaaa ccggacatga ccggttttgt tgtgattgtg aggtgagagt
      301 ttttgcgagg tgaggagtgg ctacgcagac tgaagttgcc aggcatttaa gtctgaccga
      361 tcgccagctt cgcagattgc agaaattgcc gggtgccccg atatcgaata agcgagggca
      421 actggatctg gatgcctggc gcgattttta catatcgtat ctgaggagaa gtaaaaacga
      481 tgtgcctgat ggcgatagcg aagacgacta tgaggagaaa ttgcttattg ccagatggga
      541 actgacagca gaacaggctg ttacacagca gttaaa
//
LOCUS       DBFIFS010000547          558 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.558, whole genome shotgun sequence.
ACCESSION   DBFIFS010000547 DBFIFS010000000
VERSION     DBFIFS010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.558"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            38..>558
                     /locus_tag="N7A07_005166"
     CDS             38..>558
                     /locus_tag="N7A07_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2479602.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAG"
BASE COUNT          134 a          142 c          134 g          148 t
ORIGIN      
        1 tcgttctata ttaactctaa atttcaagga tattaatatg ttttctactt tcaaaaaagc
       61 agctctgctg gcagctattg cattaccttt ttcaactatg gctgcgccta cagtcacttt
      121 tcagggtgaa gtaaccgatc agacctgttc cgtaaatatc aacggtcaaa ccaattcagt
      181 agtattgatg ccgaccgtag ccatggctga cttcggtgca actttagctg atggtcagag
      241 cgcaggccag acgccgttta cggtttctgt gtctaactgc caggctccaa ctggtgcaga
      301 tcaggcaatc aacaccacct tcctgggcta cgacgttgac gctagcacgg gtgttatggg
      361 aaaccgtgat accagcagcg atgcggcgaa aggctttggc attcagttaa tggattccag
      421 cacttctggt aacccagtaa ctctggctgg cgcgactaac gtaccgggtc tgaccctgaa
      481 agttggcgat accgaagcca gctacgactt cggtgcgcgt tacttcgtta tcgatagcgc
      541 tgctgccact gccggtaa
//
LOCUS       DBFIFS010000548          555 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.559, whole genome shotgun sequence.
ACCESSION   DBFIFS010000548 DBFIFS010000000
VERSION     DBFIFS010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.559"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            202..447
                     /locus_tag="N7A07_005167"
     CDS             202..447
                     /locus_tag="N7A07_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU2479603.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
     gene            392..555
                     /locus_tag="N7A07_005168"
                     /pseudo
     CDS             392..555
                     /locus_tag="N7A07_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          200 a          123 c          128 g          104 t
ORIGIN      
        1 cacaaccctg caaaaaataa atctatataa aaaacataca gataaccatc tgcggtgata
       61 aattatctct ggcggtgttg acacaaatac cactagcggt gatactaagc acatcagcag
      121 gacgcactaa ccaccatgaa ggtgatgctc ttaaaaatta agccctgaag aagggcagca
      181 ttcaaagcag aaggctttgg tgtgtgtgat acgaaacgaa gcattggccg gaagtgcgaa
      241 tccggattag cagccaatgt gccattgcgg ggtgttttcg ttcaggacta cgactcccac
      301 acacaaccaa agctaactaa caggagaatc cagatggatg cacaaacacg ccgccgcgaa
      361 cgtcgcgcag agaaacaggc tcaatggaaa gcagatcgga ttctggcttc aacatttcgc
      421 aggaatgcaa ctaagagaca ttactgaatc aaaaatttat tcagcaatgc agaaaatgac
      481 gaaccggcgt catgaggaaa actggaaact cagggcagaa gcatgcagaa aaaaagggaa
      541 acctgttcca gaata
//
LOCUS       DBFIFS010000549          550 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.560, whole genome shotgun sequence.
ACCESSION   DBFIFS010000549 DBFIFS010000000
VERSION     DBFIFS010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.560"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            80..155
                     /locus_tag="N7A07_005169"
     tRNA            80..155
                     /locus_tag="N7A07_005169"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Ile,seq:cat)
     gene            165..241
                     /locus_tag="N7A07_005170"
     tRNA            165..241
                     /locus_tag="N7A07_005170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:199..201,aa:Arg,seq:tcg)
     gene            255..331
                     /locus_tag="N7A07_005171"
     tRNA            255..331
                     /locus_tag="N7A07_005171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Arg,seq:tct)
     gene            complement(460..550)
                     /locus_tag="N7A07_005172"
                     /pseudo
     CDS             complement(460..550)
                     /locus_tag="N7A07_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          135 a          137 c          142 g          136 t
ORIGIN      
        1 gtcagggaag ttcaggattt agtcagtcag aacggatgat attgcaggat tagttacgta
       61 ccgttattat cctgcgcccg gccctttagc tcagtggtga gagcgagcga ctcataatcg
      121 ccaggtcgct ggttcaaatc cagcaagggc caccatatca cataccgcca ttagctcatc
      181 aggacagagc gccagccttc gaagctggtt gcgcggggtt cgggtccccg atggcggtcc
      241 attatcggta ttcagcgttg ttagctcagc cggacagagc aattgccttc taagcaatcg
      301 gtcactggtc cgaatccagt acaacgcgcc acaccacact tatctgccct gactctcttt
      361 tgcgggcttt ttattacagg aaagacaccg gacagtgaaa tgttaaatgc ctcacaattc
      421 aggcagttga atgttgcctg acatgctgag cgtttgttaa aaaaatttcg catggtgaat
      481 ccccctgtgc ggaggggcaa tcagcaacca ggtatatgtg ataatcgcgg attcaggtgc
      541 tgatactgaa
//
LOCUS       DBFIFS010000550          549 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.561, whole genome shotgun sequence.
ACCESSION   DBFIFS010000550 DBFIFS010000000
VERSION     DBFIFS010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.561"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            126..>549
                     /locus_tag="N7A07_005173"
     CDS             126..>549
                     /locus_tag="N7A07_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2479604.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLH"
BASE COUNT          174 a          101 c          113 g          161 t
ORIGIN      
        1 ccagaaaaaa catgcatgca ataatattct attaaaaatt gtattgtttt attcacatat
       61 catgagcggc atgttgattc ttgttaccaa tatagttcac aatatggatt aaaaaggata
      121 ataatatgcc agtcatatta aatttttcta atggaagtgt attgccggaa aatgagctgg
      181 aggctttacg acatatagca agaagcaacc agaatgacac tattactata ggaggccgta
      241 atatgaggct tcattatatc cagtttatgg atggttttag tgttgagcct attcttggtg
      301 gactttggga ccatcttggg gcaagagagg ctcatcatct cgcagaccgc ctgacaagac
      361 aacttaacgg aggtaacact tttctgcagg catacagctt atatctggag cagaggcaag
      421 ctgccccgct tgtgcaggag agcgtcataa aaacactact agatcgaata aattcgaatg
      481 catttcccgt tagtttacaa gacttttcct gtactgagga acatcttaat tgtccgataa
      541 cgctacata
//
LOCUS       DBFIFS010000551          533 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.562, whole genome shotgun sequence.
ACCESSION   DBFIFS010000551 DBFIFS010000000
VERSION     DBFIFS010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.562"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            66..341
                     /locus_tag="N7A07_005174"
     CDS             66..341
                     /locus_tag="N7A07_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HGU2479605.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(421..>533)
                     /locus_tag="N7A07_005175"
     CDS             complement(421..>533)
                     /locus_tag="N7A07_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HGU2479606.1"
                     /translation="YFNFTTLHDYNHFCDFIEFKHKNIIMNTSQYTGSSW"
BASE COUNT          215 a          115 c           82 g          121 t
ORIGIN      
        1 cttaatgata ccaacttata acggaatagc ataaaaacac ttttcatgga gcaaaggaga
       61 aaacaatgcc attttcaatc aaaaacagat tttcaagttc acaagtacat tacccggaaa
      121 tatccggtcc cataaaagac aagccagcgt caaagaactg catacttaca tcaacaacat
      181 gtaatgtaga tagctataca gtgtaccaaa aaaaagcctg tagttttgac atgcgcccac
      241 ccggcgcagg agaaagaacc ccaaaactaa aactctcagt tactgagatg acatggctat
      301 ctaaaactat agaaacagag atacacaaca caaaagaata gcaaccactt accagagaac
      361 acaaaaagcc acagacccga aacacctgac tgcaagcaac cacctccaca ggaggtggtt
      421 ttaccatgaa cttcctgtgt actgacttgt gttcataata atatttttgt gtttaaactc
      481 aataaagtca caaaaatgat tgtaatcatg caatgtagta aaattaaaat att
//
LOCUS       DBFIFS010000552          531 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.563, whole genome shotgun sequence.
ACCESSION   DBFIFS010000552 DBFIFS010000000
VERSION     DBFIFS010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.563"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..343)
                     /locus_tag="N7A07_005176"
     CDS             complement(<1..343)
                     /locus_tag="N7A07_005176"
                     /EC_number="3.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU2479607.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPN"
     gene            complement(346..>531)
                     /locus_tag="N7A07_005177"
     CDS             complement(346..>531)
                     /locus_tag="N7A07_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU2479608.1"
                     /translation="AKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKITLHKG
                     LENTYNWFLENQLQYRG"
BASE COUNT          158 a          110 c          100 g          163 t
ORIGIN      
        1 tgtttggttg taatttaaga aggaatgcta taactatata atgcgttgaa aagtctcccc
       61 ccatactatt gtcttcgtag aagtgctgcc agataccata aaacttaccc acagagagag
      121 gcaaacgaat tcctagttca atttctgtca atcgttcaaa ggctgtctgc aatttttcat
      181 ctttcaacac cctaccacca ggaacgaacc aatagccctg tgccgggcgg ttgattcgtt
      241 ttcctagcaa aatttcgcca aactcgtttt ccacaatcaa atctatagaa ataagaggag
      301 tagaccttac aattgtggca aagtcttggg aatgtaaaaa cattattacc cccgatattg
      361 aagttggttt tcaagaaacc agttgtatgt attttcaaga cccttgtgaa gggtaatttt
      421 atgattccaa cctagttgat gaagaagcgt tacatcaagt agttttcgag gggctccatc
      481 gggctttgtt gtatcgaacg taatatgccc tttataacct acaacttttg c
//
LOCUS       DBFIFS010000553          514 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.denovo.564, whole genome shotgun sequence.
ACCESSION   DBFIFS010000553 DBFIFS010000000
VERSION     DBFIFS010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.denovo.564"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..433)
                     /locus_tag="N7A07_005178"
     CDS             complement(<1..433)
                     /locus_tag="N7A07_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2479609.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKN"
BASE COUNT          146 a           98 c          107 g          163 t
ORIGIN      
        1 aattctttgc gccaccaatg ctctctttgt atcccgcatt aacttttatg gcaggtgggg
       61 cattgtgtgt atgtgcgtct gttgatgagt ttattacatg attttgtatg ttaatgtcat
      121 tatggatctt gaatcctctt ctcggcactg ttgcaataag cgagtgggta gtatcaccaa
      181 cagcgcgaaa tccgcgtctg ataatggata tgttctgata taacgtgtta gtaggcacac
      241 gcataccttc ttcttcccag actctggtga aaaaatcttg ttgtgcaacc acatcaggaa
      301 aagcttcaag tagcagtgat aaacaacgac tggctggcgt tgtcagaacg acgttaagat
      361 cggcgttatg taccgatatt aattttcggt gctcaggcca gaactcgata ttatcgttaa
      421 taatccagta catacgtcaa taaacactaa atcaatcgaa atggaaatca catagtttgc
      481 taagtatatt ggtatttacg gcataaatat atat
//
LOCUS       DBFIFS010000554         4549 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.282, whole genome shotgun sequence.
ACCESSION   DBFIFS010000554 DBFIFS010000000
VERSION     DBFIFS010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.282"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            complement(<1..1061)
                     /gene="pepD"
                     /locus_tag="N7A07_005179"
     CDS             complement(<1..1061)
                     /gene="pepD"
                     /locus_tag="N7A07_005179"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HGU2479610.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMT"
     gene            1322..1780
                     /gene="gpt"
                     /locus_tag="N7A07_005180"
     CDS             1322..1780
                     /gene="gpt"
                     /locus_tag="N7A07_005180"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2479611.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1872..3116
                     /gene="frsA"
                     /locus_tag="N7A07_005181"
     CDS             1872..3116
                     /gene="frsA"
                     /locus_tag="N7A07_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU2479612.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            3174..3575
                     /gene="crl"
                     /locus_tag="N7A07_005182"
     CDS             3174..3575
                     /gene="crl"
                     /locus_tag="N7A07_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU2479613.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3614..>4549)
                     /gene="phoE"
                     /locus_tag="N7A07_005183"
     CDS             complement(3614..>4549)
                     /gene="phoE"
                     /locus_tag="N7A07_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU2479614.1"
                     /translation="MHYMSDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGN
                     KAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDN
                     FMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGS
                     DFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPIT
                     GGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNK
                     NMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1098 a         1132 c         1145 g         1174 t
ORIGIN      
        1 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggag
       61 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
      121 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
      181 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagggcg
      241 tcgactttat cagctgcgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
      301 agtgtgccgc cgttgaaatc aataagacgc aggtccagtt cttctgcatg acccgccagg
      361 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcccc gccggagtga
      421 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
      481 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cgcaacccat gtagatttca
      541 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
      601 ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
      661 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
      721 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatatatgg ctggatagga
      781 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
      841 tgcaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
      901 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
      961 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     1021 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     1081 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     1141 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
     1201 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
     1261 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
     1321 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
     1381 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
     1441 ggtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     1501 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     1561 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     1621 tcgtgaaatg tatccaaaag cgcactttgt caccatcttt gcaaaaccgg ctggtcgtcc
     1681 gctggttgat gattatgttg ttgatatccc gcaagatacc tggatcgaac agccgtggga
     1741 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     1801 gccgggcgtt gttcttttta acttcaggcg agttacaata gtttccagta agtattctgg
     1861 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     1921 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     1981 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     2041 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     2101 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     2161 ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt gcggaagaag
     2221 atccgcaact cagtggtcgt cactggctgc atgcggctac gttgtacaac attgccgcct
     2281 atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttgtca aaccgcgcct
     2341 atgaagaggc cgctcagcgt ctaccgggca cgatgcgaca gatggagttt accgtacccg
     2401 gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacgg
     2461 tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt
     2521 attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttctt
     2581 caaaatggaa gctcacccag gactcctgcc tgttgcatca gcgcgtctta aaggctctgc
     2641 ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta
     2701 acgttgccgt gcgtctggca tatcttgaat cgccgcgtct gaaagcggtt gcctgtcttg
     2761 gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt
     2821 atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa gcgttgcgcg
     2881 tggagctgaa tcgctattca ttaaaagtgc aaggattgct gggacgtcga tgcccaacgc
     2941 caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa
     3001 tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg gtgtatcgga
     3061 attttgacaa aggtcttcag gaaatcaccg actggatcga aaaacgcttg tgttaaaaat
     3121 ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata gcaatgacgt
     3181 taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgtacta ggcccgtata
     3241 ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct gtatgcgtca
     3301 acgtgaaacc ggcaccggaa gtgcgtgagt tctggggctg gtggatggag cttgaagcgc
     3361 aggaatcccg ttttacctac agttaccagt ttggtctgtt cgataaagca ggcgactgga
     3421 agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc ctgcgtgagt
     3481 ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagttggaa ccggcggatg
     3541 attttcgtga cgagccggtg aagttaacgg cgtgagtgaa atgtgccgga tgcatcacat
     3601 ccggcaataa tcattaaaac tgataggtca tgccaaccgc gacaatatca tcattattaa
     3661 tattcaattt gttatcgcta tccagttggt tgattttata atcaacaaac gctgacatat
     3721 ttttgttgaa ataatatgta gccccgacat cgatataatt gaccagatct tcatcaccga
     3781 taccttcaat atctttccct ttcgataaga cataacccag cgatggacgc agaccaaagt
     3841 caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag ccgccagtta
     3901 ttggcgtcat tttgcgtgtt tcagaataaa aagttgccag ataaatatta ttggcatcgt
     3961 atttcagacc tgtagcccaa gcttctgcac gcttgcctgt gccacggctt tgcaggttct
     4021 gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa
     4081 agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg tcgcggtttt
     4141 cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg aagaagtcgg
     4201 tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg gtctgcgccg
     4261 aggagtcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca tacaacgcgc
     4321 ccaggttacg gccatagtcg aaagaaccca aatctttata cttcaatccg gcaaaagcga
     4381 gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac tccgcttccc
     4441 aacgaccata accagtcagt tgatcgttaa tttgtgtttc gcctttaaaa ccaaaacgga
     4501 tataactctg gtcgccatct ttactgtcgt tatcactcat ataatgcat
//
LOCUS       DBFIFS010000555         1761 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.400, whole genome shotgun sequence.
ACCESSION   DBFIFS010000555 DBFIFS010000000
VERSION     DBFIFS010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.400"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            <1..179
                     /gene="frdD"
                     /locus_tag="N7A07_005184"
     CDS             <1..179
                     /gene="frdD"
                     /locus_tag="N7A07_005184"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU2479615.1"
                     /translation="FIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLA
                     AILTVVTLIGVVTI"
     gene            242..1375
                     /gene="ampC"
                     /locus_tag="N7A07_005185"
     CDS             242..1375
                     /gene="ampC"
                     /locus_tag="N7A07_005185"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU2479616.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1463..>1761
                     /gene="blc"
                     /locus_tag="N7A07_005186"
     CDS             1463..>1761
                     /gene="blc"
                     /locus_tag="N7A07_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU2479617.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPT"
BASE COUNT          442 a          454 c          440 g          425 t
ORIGIN      
        1 gcttcattgg tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac
       61 accgtatgca ccacgcgatg cacgatctga aaatccacgt acctgcgggc aaatgggttt
      121 tctacggtct ggctgctatc ctgacagttg tcacgctgat tggtgtcgtt acaatctaac
      181 gcatcgccaa tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagaccc
      241 tatgttcaaa acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc
      301 ccctcaacaa atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa
      361 gatccccggt atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg
      421 gtatgcggac atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc
      481 ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa
      541 gttaagcgat cccgcaacaa aatactggcc tgaacttacc gctaaacagt ggaatgggat
      601 cacactatta catctcgcaa cctacactgc tggcggcctg ccattgcagg tgccggatga
      661 agtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggctcc
      721 aggaacacaa cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa
      781 gccgtctggt ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact
      841 caaccatacg tggattaatg taccgcccgc agaagaaaag aattacgcct ggggatatcg
      901 cgaaggcaag gcagttcatg tttcgccagg ggcgttagat gctgaaactt atggtgtgaa
      961 gtcgaccatt gaagatatgg cctgctgggt acgaagcaat atgaatcccc gtgatatcaa
     1021 cgacaaaaca cttcagcaag ggatacaact ggcacaatct cgctactggc aaaccggcga
     1081 tatgtatcag ggtctgggct gggaaatgct ggactggccg gtaaatcctg acatcatcgt
     1141 taacggcagt gacaataaaa ttgcactggc agcacgcccc gtaaaagcga ttacgcctcc
     1201 aactcctgca gtacgagcat catgggtgca taaaacgggg gcgaccggcg gatttggtag
     1261 ctatgtcgcg tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta
     1321 tcccaatcca gcgagagtcg ccgccgcctg gcagattctc aacgctctac agtaaaattt
     1381 catcgggtcc gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga
     1441 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
     1501 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa
     1561 tttcgacgct aaacgctatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
     1621 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
     1681 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
     1741 gtactttacc ggcgcaccaa c
//
LOCUS       DBFIFS010000556         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.438, whole genome shotgun sequence.
ACCESSION   DBFIFS010000556 DBFIFS010000000
VERSION     DBFIFS010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.438"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005187"
     CDS             6..965
                     /locus_tag="N7A07_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479618.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005188"
     CDS             977..1246
                     /locus_tag="N7A07_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2479619.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          369 a          259 c          328 g          378 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000557         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.439, whole genome shotgun sequence.
ACCESSION   DBFIFS010000557 DBFIFS010000000
VERSION     DBFIFS010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.439"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005189"
     CDS             6..965
                     /locus_tag="N7A07_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479620.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005190"
     CDS             977..1246
                     /locus_tag="N7A07_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU2479621.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          371 a          259 c          326 g          378 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000558         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.440, whole genome shotgun sequence.
ACCESSION   DBFIFS010000558 DBFIFS010000000
VERSION     DBFIFS010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.440"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005191"
     CDS             6..965
                     /locus_tag="N7A07_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479622.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005192"
     CDS             977..1246
                     /locus_tag="N7A07_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2479623.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          368 a          260 c          327 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000559         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.441, whole genome shotgun sequence.
ACCESSION   DBFIFS010000559 DBFIFS010000000
VERSION     DBFIFS010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.441"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005193"
     CDS             6..965
                     /locus_tag="N7A07_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479624.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005194"
     CDS             977..1246
                     /locus_tag="N7A07_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU2479625.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          370 a          260 c          325 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000560         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.442, whole genome shotgun sequence.
ACCESSION   DBFIFS010000560 DBFIFS010000000
VERSION     DBFIFS010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.442"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005195"
     CDS             6..965
                     /locus_tag="N7A07_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479626.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005196"
     CDS             977..1246
                     /locus_tag="N7A07_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2479627.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          368 a          260 c          328 g          378 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acgaacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000561         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.443, whole genome shotgun sequence.
ACCESSION   DBFIFS010000561 DBFIFS010000000
VERSION     DBFIFS010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.443"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005197"
     CDS             6..965
                     /locus_tag="N7A07_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479628.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005198"
     CDS             977..1246
                     /locus_tag="N7A07_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU2479629.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          370 a          260 c          326 g          378 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acgaacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000562         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.444, whole genome shotgun sequence.
ACCESSION   DBFIFS010000562 DBFIFS010000000
VERSION     DBFIFS010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.444"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005199"
     CDS             6..965
                     /locus_tag="N7A07_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479630.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005200"
     CDS             977..1246
                     /locus_tag="N7A07_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2479631.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          367 a          261 c          327 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acgaacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFS010000563         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 79_160608
            SAMD00495951-rid25093773.guided.445, whole genome shotgun sequence.
ACCESSION   DBFIFS010000563 DBFIFS010000000
VERSION     DBFIFS010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495951
            Sequence Read Archive: DRR381687
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424395.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424395.2
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,873
            CDSs (with protein)               :: 4,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 98 of 235
            Pseudo Genes (incomplete)         :: 120 of 235
            Pseudo Genes (internal stop)      :: 63 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495951-rid25093773.guided.445"
                     /strain="79_160608"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-05-20"
     gene            6..965
                     /locus_tag="N7A07_005201"
     CDS             6..965
                     /locus_tag="N7A07_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2479632.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7A07_005202"
     CDS             977..1246
                     /locus_tag="N7A07_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU2479633.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          369 a          261 c          325 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acgaacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
